FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Xie, JL Qi, JQ Hse, CY Shupe, TF AF Xie, Jiulong Qi, Jinqiu Hse, Chungyun Shupe, Todd F. TI Optimization for microwave-assisted direct liquefaction of bamboo residue in glycerol/methanol mixtures SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Bamboo residue; Microwave; Liquefaction; Glycerol ID WOOD LIQUEFACTION; POLYURETHANE FOAM; FEEDSTOCKS; PRODUCTS AB Bamboo residues were liquefied in a mixture of glycerol and methanol in the presence of sulfuric acid using microwave energy. We investigated the effects of liquefaction conditions, including glycerol/methanol ratio, liquefaction temperature, and reaction time on the conversion yield. The optimal liquefaction conditions were under the temperature of 120 A degrees C, the reaction time of 7 min, the glycerol-methanol-bamboo ratio of 8/0/2 (W/W), and the microwave power of 300 W. Maximum conversion yield was 96.7 %. The liquid products were separated into two contents (water soluble part and precipitate part) by addition of a sufficient amount of water. By Fourier transform infrared (FT-IR), the water soluble content mainly contained glycerol and its derivate and carbohydrate degradation products, and the precipitate content was mainly lignin derivatives. C1 [Xie, Jiulong; Qi, Jinqiu] Sichuan Agr Univ, Coll Forestry, Yaan 625014, Sichuan, Peoples R China. [Hse, Chungyun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Shupe, Todd F.] Louisiana State Univ, Sch Renewable Nat Resource, Ctr Agr, Baton Rouge, LA 70803 USA. RP Qi, JQ (reprint author), Sichuan Agr Univ, Coll Forestry, Yaan 625014, Sichuan, Peoples R China. EM qijinqiu2005@aliyun.com FU Key Laboratory of Wood Industry and Furniture Engineering of Sichuan Provincial Colleges and Universities FX The project was supported by "Key Laboratory of Wood Industry and Furniture Engineering of Sichuan Provincial Colleges and Universities". NR 14 TC 6 Z9 7 U1 3 U2 28 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD MAR PY 2015 VL 26 IS 1 BP 261 EP 265 DI 10.1007/s11676-015-0032-1 PG 5 WC Forestry SC Forestry GA CB4MO UT WOS:000349602100030 ER PT J AU Zhang, HL Scharfenstein, L Zhang, DH Chang, PK Montalbano, BG Guo, BZ Meng, XJ Yu, JJ AF Zhang, Huili Scharfenstein, Leslie Zhang, Dunhua Chang, Perng-Kuang Montalbano, Beverly G. Guo, Baozhu Meng, Xianjun Yu, Jiujiang TI Peanut Resistance Gene Expression in Response to Aspergillus flavus Infection During Seed Germination SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE Aspergillus flavus; fungal resistance; gene expression; peanut; plant-fungus interaction; real-time PCR ID DESATURASE MESSENGER-RNA; AFLATOXIN CONTAMINATION; OXALATE OXIDASE; DROUGHT STRESS; MAIZE; IDENTIFICATION; PROTEINS; DEFENSE; PATHWAY; PLANTS AB Aspergillus flavus produces potent mutagenic and carcinogenic polyketide-derived secondary metabolites known as aflatoxins. Development of host plant resistance in peanut and other crops is the most environmentally friendly and cost-effective method to eliminate the serious problem of aflatoxin contamination in grains. To confirm that putative peanut genes identified in a previous microarray study were involved in peanut resistance to A.flavus infection, 14 genes were selected for further investigation through real-time PCR. The results revealed diverse patterns of gene expression during seed germination after A.flavus inoculation. Based on the expression levels and the relative-expression patterns over a 7-day period, the 14 host genes could be classified into six different groups belonging to three main biochemical and genetic defence processes of lipid metabolism, oxidative signalling and cell-wall synthesis during counter-attack. A network of gene expression patterns was activated in sequential order in response to A.flavus invasion in both resistant and susceptible peanut lines during seed germination. Understanding gene expression patterns in peanut will be useful to breeders and other scientists interested in incorporating genetic resources of resistance against A.flavus into peanut germplasm and/or commercial cultivars via conventional and/or molecular methods. C1 [Zhang, Huili] Liaoning Univ, Light Ind Coll, Shenyang 110036, Peoples R China. [Scharfenstein, Leslie; Chang, Perng-Kuang; Montalbano, Beverly G.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Zhang, Dunhua] USDA ARS, Auburn, AL 36832 USA. [Guo, Baozhu] USDA ARS, Tifton, GA 31793 USA. [Meng, Xianjun] Shenyang Agr Univ, Coll Food Sci, Shenyang 110161, Peoples R China. [Yu, Jiujiang] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Yu, JJ (reprint author), USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM Jiujiang.yu@ars.usda.gov FU Science and Technology Department of Liaoning Province [201401186]; Education Department of Liaoning Province [2014014] FX The authors declare that there has no conflict of interest. Project related: Science and Technology Department of Liaoning Province (No. 201401186); Education Department of Liaoning Province (No. 2014014). NR 60 TC 4 Z9 5 U1 1 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD MAR PY 2015 VL 163 IS 3 BP 212 EP 221 DI 10.1111/jph.12311 PG 10 WC Plant Sciences SC Plant Sciences GA CB7BK UT WOS:000349780900008 ER PT J AU Li, C Gowan, S Anil, A Beck, BH Thongda, W Kucuktas, H Kaltenboeck, L Peatman, E AF Li, Chao Gowan, Spencer Anil, Ammu Beck, Benjamin H. Thongda, Wilawan Kucuktas, Huseyin Kaltenboeck, Ludmilla Peatman, Eric TI Discovery and validation of gene-linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus) SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE bass; hybridization; introgression; Micropterus; single nucleotide polymorphism ID BIMODAL HYBRID ZONES; MICROSATELLITE LOCI; CUTTHROAT TROUT; BLACK BASSES; SPECIATION; PROTEIN; POPULATIONS; EXPRESSION; NORTHERN; DNA AB Efforts to improve recreational fisheries have included widespread stocking of Micropterus floridanus outside its native range of peninsular Florida. Hybridization of Florida bass (M.floridanus) with largemouth bass (Micropterus salmoides) has now dramatically expanded beyond a naturally occurring intergrade zone in the southeast U.S. In recent years, there has been growing interest in protecting the genetic integrity of native basses and assessing the impact and nature of M.salmoides/M.floridanus introgression from the standpoint of hatchery and sport-fishery managers, fish biologists, ecologists and evolutionary biologists. Here, we conducted RNA-seq-based sequencing of the transcriptomes of M.salmoides, M.floridanus and their F1 hybrid and identified a set of 3674 SNP markers with fixed-allelic differences from 2112 unique genes. We then developed a subset of 25 of these markers into a single diagnostic multiplex assay and validated its capacity for assessing integrity and hybridization in hatchery and wild populations of largemouth and Florida bass. The availability of this resource, high-quality transcriptomes and a large set of gene-linked SNPs, should greatly facilitate functional and population genomics studies in these key species and allow the identification of traits and processes under selection during introgressive hybridization. C1 [Li, Chao; Gowan, Spencer; Anil, Ammu; Thongda, Wilawan; Kucuktas, Huseyin; Kaltenboeck, Ludmilla; Peatman, Eric] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. [Beck, Benjamin H.] ARS, USDA, Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Peatman, E (reprint author), Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Fax 1-334-844-4694, Auburn, AL 36849 USA. EM peatmer@auburn.edu FU Alabama Department of Conservation and Natural Resources; federal Dingell-Johnson Sport Fish Restoration dollars FX We are grateful to public and private collaborators for their assistance in sample collection, particularly Damon Abernathy at the Alabama Department of Conservation and Natural Resources, Don Keller at American Sport Fish and biologists at the Florida Fish and Wildlife Conservation Commission and Georgia Department of Natural Resources. The study was conducted with funding from the Alabama Department of Conservation and Natural Resources as well as federal Dingell-Johnson Sport Fish Restoration dollars. The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 76 TC 1 Z9 1 U1 3 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X EI 1755-0998 J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD MAR PY 2015 VL 15 IS 2 BP 395 EP 404 DI 10.1111/1755-0998.12308 PG 10 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA CB7PE UT WOS:000349819000017 PM 25047482 ER PT J AU Martin, FM AF Martin, Francis M. TI Francis M. Martin SO NEW PHYTOLOGIST LA English DT Editorial Material DE ectomycorrhizas; effectors; interactions; profile; signalling pathways ID BOREAL FOREST C1 [Martin, Francis M.] Univ Paris 11, Orsay, France. [Martin, Francis M.] Univ Calif Los Angeles, Dept Biochem, Los Angeles, CA 90024 USA. [Martin, Francis M.] USDA, Plant Soil Biophys Dept, Wyndmoor, PA USA. [Martin, Francis M.] INRA Nancy, Tree Microbes Interact Dept, Champenoux, France. RP Martin, FM (reprint author), CSIRO Forestry Res Dept, Nick Malajuczuks Lab, Perth, WA, Australia. NR 4 TC 0 Z9 0 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD MAR PY 2015 VL 205 IS 4 SI SI BP 1404 EP 1405 DI 10.1111/nph.13308 PG 2 WC Plant Sciences SC Plant Sciences GA CB1KN UT WOS:000349386300009 PM 25645717 ER PT J AU Dao, TH Schomberg, HH Cavigelli, MA AF Dao, Thanh H. Schomberg, H. H. Cavigelli, M. A. TI Tillage and rotational effects on exchangeable and enzyme-labile phosphorus forms in conventional and organic cropping systems SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Enzyme-labile phosphorus; Soil organic P; Phosphorus fractionation; Conventional tillage; No-tillage; Organic farming; Sustainable nutrient management ID WASTE-WATER; SOIL; MATTER; CARBON; MANAGEMENT; MANURE; DEPHOSPHORYLATION; RHIZOSPHERE; FRACTIONS; SORPTION AB Transformations of crop residues and bio-fertilizers, used as primary sources of nutrients for organic crop production are influenced by soil management practices. The release and distribution of labile phosphorus (P) forms were studied in three organic and two conventional crop management systems after 18 years. Spatial variability and stratification in inorganic P (Pi), organic, and total P in the top 20-cm depth showed that reducing accumulation and potential transport of exchangeable inorganic (EEPi) and enzyme-labile organic P (EDTA-PHP), that averaged 17.2 and 40.4 mg kg(-1), remained a high priority in chisel-till and no-till soils. In the organic systems, annualized rates of P addition were within soil test recommendations. However, P surpluses, likely caused by yield-limiting conditions and overestimates of plant P requirements, resulted in large pools of EEPi and EDTA-PHP, averaging 30.9 and 68.2 mg kg(-1), respectively. Soil drying and rewetting enhanced extractability of all labile P forms, which included a 2.4- to 3-fold increase in EEPi. Differences in P loading between conventional and organic treatments suggested that new soil tests may be needed to assess crop needs in organic systems since current procedures do not account for presence and size of the EDTA-PHP pool. The impact of large P loadings is long-lived as these additions continued to contribute to elevated bioactive P concentrations 12 or more years later. Accurate estimates of crop requirements and lability of soil P pools, and real-time plant and soil P sensing systems are critical considerations to optimally manage manure-derived nutrients in organic production systems. C1 [Dao, Thanh H.; Schomberg, H. H.; Cavigelli, M. A.] ARS, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Dao, TH (reprint author), ARS, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave,BARC East, Beltsville, MD 20705 USA. EM thanh.dao@ars.usda.gov NR 38 TC 1 Z9 1 U1 2 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 EI 1573-0867 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD MAR PY 2015 VL 101 IS 2 BP 153 EP 165 DI 10.1007/s10705-014-9665-2 PG 13 WC Soil Science SC Agriculture GA CB4OB UT WOS:000349606200001 ER PT J AU Sauer, TJ Coblentz, WK Thomas, AL Brye, KR Brauer, DK Skinner, JV Van Brahana, J DeFauw, SL Hays, PD Moffitt, DC Robinson, JL James, TA Hickie, KA AF Sauer, Thomas J. Coblentz, Wayne K. Thomas, Andrew L. Brye, Kris R. Brauer, David K. Skinner, J. Vaughn Van Brahana, J. DeFauw, Sherri L. Hays, Phillip D. Moffitt, David C. Robinson, James L. James, Travis A. Hickie, Kevin A. TI Nutrient cycling in an agroforestry alley cropping system receiving poultry litter or nitrogen fertilizer SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Agroforestry; Poultry litter; Nutrient cycling; Eastern black walnut; Pecan; Northern red oak; Orchardgrass ID RUNOFF WATER-QUALITY; BROILER-LITTER; BLACK-WALNUT; TALL FESCUE; OZARK HIGHLANDS; MANAGEMENT; NITRATE; SOIL; USA; ORCHARDGRASS AB Optimal utilization of animal manures as a plant nutrient source should also prevent adverse impacts on water quality. The objective of this study was to evaluate long-term poultry litter and N fertilizer application on nutrient cycling following establishment of an alley cropping system with eastern black walnut (Juglans nigra L.), pecan [Carya illinoensis (Wangenh.) K. Koch], and northern red oak (Quercus rubra L.) trees and orchardgrass (Dactylis glomerata L.). One half of a 4.25-ha site in northwestern Arkansas USA received broadcast applications of 3.9-6.7 Mg ha(-1) fresh poultry litter and the other half 50-76 kg ha(-1) N as NH4NO3 fertilizer each spring from 2001 to 2008. Macronutrient (N, P, K, Ca, Mg, and S) and micronutrient (Na, Fe, Mn, Zn, and Cu) concentrations in soil, forage, and tree leaf tissue were monitored along with NO3-N in soil water and groundwater. Poultry litter application resulted in significantly increased concentrations of each macronutrient except S with increases from 6.3 (N) to 121 % (P). Nitrogen fertilizer application resulted in decreased concentrations from 2.1 (N) to 60.9 % (S) for all macronutrients except Ca. Patterns of nutrient content in forage and tree leaf tissue did not generally follow patterns of soil nutrient concentrations suggesting nutrient sufficiency in most years and that climate and plant growth had a greater effect on nutrient uptake. Soil P with litter application increased 41.3 mg kg(-1) over 7 years (from 34.1 to 75.4), which may necessitate a lower litter application rate to avoid excessive P runoff. C1 [Sauer, Thomas J.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA 50011 USA. [Coblentz, Wayne K.] ARS, US Dairy Forage Res Ctr, USDA, Marshfield, WI USA. [Thomas, Andrew L.] Univ Missouri Columbia, Southwest Res Ctr, Mt Vernon, MO USA. [Brye, Kris R.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Brauer, David K.] ARS, Conservat & Prod Res Lab, USDA, Bushland, TX USA. [Skinner, J. Vaughn] Univ Arkansas, Agr Expt Stn, Fayetteville, AR 72701 USA. [Van Brahana, J.] Univ Arkansas, Dept Geosci, Fayetteville, AR 72701 USA. [DeFauw, Sherri L.] Penn State Univ, Dept Agr Econ Sociol & Educ, University Pk, PA 16802 USA. [Hays, Phillip D.] US Geol Survey, Fayetteville, AR USA. [Moffitt, David C.] Texas A&M AgriLife Res & Extens Ctr, Temple, TX USA. [Robinson, James L.] NRCS, USDA, Ft Worth, TX USA. [James, Travis A.] NRCS, USDA, Salt Lake City, UT USA. [Hickie, Kevin A.] Arkansas Forestry Commiss, Fayetteville, AR USA. RP Sauer, TJ (reprint author), ARS, Natl Lab Agr & Environm, USDA, 2110 Univ Blvd, Ames, IA 50011 USA. EM tom.sauer@ars.usda.gov OI Hays, Phillip/0000-0001-5491-9272 FU U.S. Forest Service; Natural Resources Conservation Service FX The authors are grateful to Paul Doi, Amy Morrow, Anna Myhre, and Kevin Jensen (National Laboratory for Agriculture and the Environment), Nancy Wolf (Univ. of Arkansas Agricultural Diagnostics Lab), Tammy Horton (Dale Bumpers Small Farms Research Center), Amanda Pirani (Univ. of Arkansas, Dept. of Crop, Soil and Environmental Sciences) and Robert Rhein, Dr. Keith Lusby, and Dr. Dirk Philipp (Univ. of Arkansas, Dept. of Animal Science) for their assistance in completion of this study. Financial support from the U.S. Forest Service and Natural Resources Conservation Service is also gratefully acknowledged. NR 39 TC 0 Z9 0 U1 4 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 EI 1573-0867 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD MAR PY 2015 VL 101 IS 2 BP 167 EP 179 DI 10.1007/s10705-014-9667-0 PG 13 WC Soil Science SC Agriculture GA CB4OB UT WOS:000349606200002 ER PT J AU Witbracht, M Keim, NL Forester, S Widaman, A Laugero, K AF Witbracht, Megan Keim, Nancy L. Forester, Shavawn Widaman, Adrianne Laugero, Kevin TI Female breakfast skippers display a disrupted cortisol rhythm and elevated blood pressure SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Cortisol; Breakfast skipping; Blood pressure; Cardiometabolic health ID CARDIOMETABOLIC RISK-FACTORS; BODY-MASS INDEX; PITUITARY-ADRENAL AXIS; METABOLIC SYNDROME; US ADULTS; CARDIOVASCULAR RISK; SKIPPING BREAKFAST; CEREAL CONSUMPTION; ABDOMINAL OBESITY; CUSHINGS-SYNDROME AB Chronic stress and over-activity in the hypothalamic-pituitary-adrenal (HPA) axis may link breakfast skipping and poor cardiometabolic health. Missing the first major meal of the day in rodents prolongs elevated circulating corticosterone at a time when it's normally decreasing. To extend these findings to humans, we hypothesized that habitual breakfast skippers would display a similar pattern of circulating cortisol and alterations in meal and stress-induced cortisol reactions. Normal weight to obese women aged 18-45 years old who were strictly defined as either breakfast skippers (n = 30) or breakfast eaters (n = 35) were invited to participate in our study. Normal breakfast habits were maintained for the entire study period and each participant attended 4 lab visits. Over the first 2 lab visits, body composition, fasting clinical chemistries, and self-reports of chronic stress were assessed. On each of 2 additional days (lab visits 3 and 4), salivary free cortisol was measured at home upon waking and at bedtime, and in the lab in response to a standard lunch, ad libitum afternoon snack buffet, and stress and control (relaxation) tasks. The order of the control and stress test visits was randomized. While body weight, body composition, HOMA-IR, total and HDL cholesterol did not statistically differ (p > 0.05), both diastolic and systolic blood pressure was elevated (p < 0.01) and LDL cholesterol was lower (p = 0.04) in the breakfast skipper group. Compared to the breakfast eaters and on the control task visit only, breakfast skippers had higher circulating cortisol from arrival to midafternoon (p < 0.01) and during the snack buffet (p < 0.05). Furthermore, the lunch-induced cortisol reaction was larger in the 'skippers' (p = 0.03). On both stress and control visit days, the diurnal cortisol amplitude was significantly (p = 0.02) blunted in breakfast skippers. Self-reports of chronic stress did not differ between the groups. These data indicate that habitually skipping breakfast is associated with stress-independent over-activity in the HPA axis which, if prolonged, may increase risk (e.g., hypertension) for cardiometabolic disease in some people. (C) 2014 Published by Elsevier Inc. C1 [Witbracht, Megan; Keim, Nancy L.; Widaman, Adrianne; Laugero, Kevin] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Keim, Nancy L.; Laugero, Kevin] ARS, USDA, Western Human Nutr Res Ctr, Obes & Metab Res Unit, Davis, CA USA. [Forester, Shavawn] Tahoe Forest Hosp, Truckee, CA 96161 USA. RP Laugero, K (reprint author), ARS, Stress Biol & Nutr Res Lab, Obes & Metab Res Unit, Western Human Nutr Res Ctr,USDA, Davis, CA USA. EM kevin.laugero@ars.usda.gov FU USDA-CSREES NRI grant [2009-35215-05364]; USDA Agricultural Research Service intramural project [5306-51530-019-00] FX This research was supported with funding from USDA-CSREES NRI grant 2009-35215-05364 and USDA Agricultural Research Service intramural project number 5306-51530-019-00. NR 67 TC 8 Z9 8 U1 4 U2 25 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD MAR 1 PY 2015 VL 140 BP 215 EP 221 DI 10.1016/j.physbeh.2014.12.044 PG 7 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA CB4HI UT WOS:000349588400029 PM 25545767 ER PT J AU Funk, PA AF Funk, P. A. TI Reducing cyclone pressure drop with evases SO POWDER TECHNOLOGY LA English DT Article DE Cyclone; Energy recovery; Evase; Pressure regain; Pressure drop; Return on investment ID COMPUTATIONAL FLUID-DYNAMICS; ARTIFICIAL NEURAL-NETWORKS; FLOW PATTERN; GENETIC ALGORITHMS; PERFORMANCE; GAS; CFD; SEPARATORS; OPTIMIZATION; EMISSIONS AB Cyclones are widely used to separate particles from gas flows and as air emission control devices. Their cost of operation is proportional to the fan energy required to overcome their pressure drop. Evases or exit diffusers potentially could reduce exit pressure losses without affecting collection efficiency. Three rectangular evases and a radial evase with a variable opening were tested on two cyclones. Pressure drop was recorded for inlet velocities from about 10 to 20 m s(-1). The radial evase reduced cyclone pressure drop by between 8.7 and 11.9 percent when its exit area was equal to the flow area of the cyclone vortex finder or gas exit. A simple payback based on avoided energy costs was estimated to be between 3600 and 5000 h, not including installation cost. Published by Elsevier B.V. C1 ARS, USDA, Southwestern Cotton Ginning Res Lab, Park, NM 88047 USA. RP Funk, PA (reprint author), ARS, USDA, Southwestern Cotton Ginning Res Lab, 300 E Coll Dr,POB 578, Park, NM 88047 USA. EM paul.funk@ars.usda.gov NR 49 TC 0 Z9 0 U1 2 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0032-5910 EI 1873-328X J9 POWDER TECHNOL JI Powder Technol. PD MAR PY 2015 VL 272 BP 276 EP 281 DI 10.1016/j.powtec.2014.12.019 PG 6 WC Engineering, Chemical SC Engineering GA CB4BP UT WOS:000349573500028 ER PT J AU Kleinman, PJA Sharpley, AN Withers, PJA Bergstrom, L Johnson, LT Doody, DG AF Kleinman, Peter J. A. Sharpley, Andrew N. Withers, Paul J. A. Bergstrom, Lars Johnson, Laura T. Doody, Donnacha G. TI Implementing agricultural phosphorus science and management to combat eutrophication SO AMBIO LA English DT Article; Proceedings Paper CT 7th International Phosphorus Workshop (IPW) CY SEP, 2013 CL Uppsala, SWEDEN DE Eutrophication; Mitigation measures; No till; Phosphorus management; Tile drainage ID WATER-QUALITY; LAKE-ERIE; BUFFER STRIPS; GREAT-LAKES; CATCHMENT; SOIL; LANDSCAPES; GRASSLAND; POLLUTION; TRANSFERS AB Experience with implementing agricultural phosphorus (P) strategies highlights successes and uncertainty over outcomes. We examine case studies from the USA, UK, and Sweden under a gradient of voluntary, litigated, and regulatory settings. In the USA, voluntary strategies are complicated by competing objectives between soil conservation and dissolved P mitigation. In litigated watersheds, mandated manure export has not wrought dire consequences on poultry farms, but has adversely affected beef producers who fertilize pastures with manure. In the UK, regulatory and voluntary approaches are improving farmer awareness, but require a comprehensive consideration of P management options to achieve downstream reductions. In Sweden, widespread subsidies sometime hinder serious assessment of program effectiveness. In all cases, absence of local data can undermine recommendations from models and outside experts. Effective action requires iterative application of existing knowledge of P fate and transport, coupled with unabashed description and demonstration of tradeoffs to local stakeholders. C1 [Kleinman, Peter J. A.] ARS, USDA, Pasture Syst & Watershed Management Unit, University Pk, PA 16802 USA. [Sharpley, Andrew N.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Withers, Paul J. A.] Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2DG, Gwynedd, Wales. [Bergstrom, Lars] Swedish Univ Agr Sci, Dept Soil & Environm, S-75007 Uppsala, Sweden. [Johnson, Laura T.] Heidelberg Univ, Natl Ctr Water Qual Res, Tiffin, OH 44883 USA. [Doody, Donnacha G.] Agri Food & Biosci Inst, Belfast 8T9 5PX, Antrim, North Ireland. RP Kleinman, PJA (reprint author), ARS, USDA, Pasture Syst & Watershed Management Unit, University Pk, PA 16802 USA. EM peter.kleinman@ars.usda.gov; sharpley@uark.edu; p.withers@bangor.ac.uk; lars.bergstrom@slu.se; ljohnson@heidelberg.edu; donnacha.doody@afbini.gov.uk NR 70 TC 24 Z9 24 U1 11 U2 115 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0044-7447 EI 1654-7209 J9 AMBIO JI Ambio PD MAR PY 2015 VL 44 SU 2 SI SI BP S297 EP S310 DI 10.1007/s13280-015-0631-2 PG 14 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CB1TT UT WOS:000349411600012 PM 25681986 ER PT J AU Li, HG Liu, J Li, GH Shen, JB Bergstrom, L Zhang, FS AF Li, Haigang Liu, Jian Li, Guohua Shen, Jianbo Bergstrom, Lars Zhang, Fusuo TI Past, present, and future use of phosphorus in Chinese agriculture and its influence on phosphorus losses SO AMBIO LA English DT Article; Proceedings Paper CT 7th International Phosphorus Workshop (IPW) CY SEP, 2013 CL Uppsala, SWEDEN DE Phosphorus management zone; Olsen P; Environment; Crop yield; Phosphorus loss ID SOIL TEST PHOSPHORUS; FERTILIZER PHOSPHORUS; MANURE PHOSPHORUS; WATER-QUALITY; SOUTH CHINA; RUNOFF LOSS; FIELD; NITROGEN; DYNAMICS; RISK AB Large inputs of phosphorus (P) in chemical fertilizers and feed supplements since 1978 have improved soil P status in arable land in China, but have also created challenges by increasing P concentrations in manure and exacerbating water quality degradation. Arable land in China can be divided into five management zones based on soil P chemistry, with 15-92 % of arable land having lower P status than the agronomic optimum and 0.3-7.2 % having severe risks of P leaching losses. A scenario analysis of soil P budget and agronomic P demand during 2011-2030 highlighted the great pressure China faces in sustainable P management and the need for drastic changes in current practices. This includes new policies to reduce P supplementation of feed and improved P use efficiency by livestock and programs to expand the adoption of appropriate fertilization, soil conservation, and drainage management practices to minimize P losses. C1 [Li, Haigang; Li, Guohua; Shen, Jianbo; Zhang, Fusuo] China Agr Univ, CREFS, Beijing 100193, Peoples R China. [Liu, Jian] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Nonpoint Source Pollut Control, Beijing 100081, Peoples R China. [Liu, Jian] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA 16802 USA. [Bergstrom, Lars] Swedish Univ Agr Sci SLU, Dept Soil & Environm, S-75007 Uppsala, Sweden. RP Zhang, FS (reprint author), China Agr Univ, CREFS, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. EM haigangli@cau.edu.cn; jianliu1985yy@163.com; liguohuatiancai@163.com; jbshen@cau.edu.cn; lars.bergstrom@slu.se; zhangfs@cau.edu.cn FU National Basic Research Program of China [973-2015CB150405]; Innovative Group Grant of the National Natural Science Foundation of China [31421092]; Special Fund for Agro-scientific Research in the Public Interest [201003014]; National Natural Science Foundation of China [31201691, 30925024, 31330070] FX This research was supported by the National Basic Research Program of China (973-2015CB150405), the Innovative Group Grant of the National Natural Science Foundation of China (31421092), the Special Fund for Agro-scientific Research in the Public Interest (201003014), and the National Natural Science Foundation of China (31201691, 30925024, 31330070). NR 78 TC 10 Z9 14 U1 18 U2 89 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0044-7447 EI 1654-7209 J9 AMBIO JI Ambio PD MAR PY 2015 VL 44 SU 2 SI SI BP S274 EP S285 DI 10.1007/s13280-015-0633-0 PG 12 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CB1TT UT WOS:000349411600010 PM 25681984 ER PT J AU Sharpley, AN Bergstrom, L Aronsson, H Bechmann, M Bolster, CH Borling, K Djodjic, F Jarvie, HP Schoumans, OF Stamm, C Tonderski, KS Ulen, B Uusitalo, R Withers, PJA AF Sharpley, Andrew N. Bergstrom, Lars Aronsson, Helena Bechmann, Marianne Bolster, Carl H. Borling, Katarina Djodjic, Faruk Jarvie, Helen P. Schoumans, Oscar F. Stamm, Christian Tonderski, Karin S. Ulen, Barbro Uusitalo, Risto Withers, Paul J. A. TI Future agriculture with minimized phosphorus losses to waters: Research needs and direction SO AMBIO LA English DT Article; Proceedings Paper CT 7th International Phosphorus Workshop (IPW) CY SEP, 2013 CL Uppsala, SWEDEN DE Implementation; Manure; Mitigation measures; Monitoring; P management; Transport pathways ID HYDROLOGICAL PATHWAYS; UNCERTAINTY ANALYSIS; SOIL-PHOSPHORUS; SNOWMELT RUNOFF; MANAGEMENT; CATCHMENTS; QUALITY; CHALLENGES; SURFACE; MODELS AB The series of papers in this issue of AMBIO represent technical presentations made at the 7th International Phosphorus Workshop (IPW7), held in September, 2013 in Uppsala, Sweden. At that meeting, the 150 delegates were involved in round table discussions on major, predetermined themes facing the management of agricultural phosphorus (P) for optimum production goals with minimal water quality impairment. The six themes were (1) P management in a changing world; (2) transport pathways of P from soil to water; (3) monitoring, modeling, and communication; (4) importance of manure and agricultural production systems for P management; (5) identification of appropriate mitigation measures for reduction of P loss; and (6) implementation of mitigation strategies to reduce P loss. This paper details the major challenges and research needs that were identified for each theme and identifies a future roadmap for catchment management that cost-effectively minimizes P loss from agricultural activities. C1 [Sharpley, Andrew N.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Bergstrom, Lars; Aronsson, Helena; Ulen, Barbro] Swedish Univ Agr Sci, Dept Soil & Environm, S-75007 Uppsala, Sweden. [Bechmann, Marianne] Bioforsk, Dept Soil & Environm, N-1430 As, Norway. [Bolster, Carl H.] ARS, USDA, Bowling Green, KY 42104 USA. [Borling, Katarina] Swedish Board Agr, S-75320 Uppsala, Sweden. [Djodjic, Faruk] Swedish Univ Agr Sci, Dept Aquat Sci & Assessment, S-75007 Uppsala, Sweden. [Jarvie, Helen P.] Ctr Ecol & Hydrol, Wallingford OX10 8BB, Oxon, England. [Schoumans, Oscar F.] Alterra Wageningen UR, Alterra, NL-6700 AA Wageningen, Netherlands. [Stamm, Christian] Eawag, CH-8600 Dubendorf, Switzerland. [Tonderski, Karin S.] Linkoping Univ, Dept Phys Chem & Biol, S-58183 Linkoping, Sweden. [Uusitalo, Risto] MTT Agrifood Res Finland, Jokioinen 31600, Finland. [Withers, Paul J. A.] Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2DG, Gwynedd, Wales. RP Sharpley, AN (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. EM sharpley@uark.edu; lars.bergstrom@slu.se; helena.aronsson@slu.se; marianne.bechmann@bioforsk.no; carl.bolster@ars.usda.gov; katarina.borling@jordbruksverket.se; Faruk.Djodjic@slu.se; hpj@ceh.ac.uk; oscar.schoumans@wur.nl; christian.stamm@eawag.ch; karsu@ifm.liu.se; barbro.ulen@slu.se; risto.uusitalo@mtt.fi; p.withers@bangor.ac.uk RI Djodjic, Faruk/I-9944-2016; OI Djodjic, Faruk/0000-0002-2172-242X; Stamm, Christian/0000-0001-5888-6535 NR 111 TC 28 Z9 29 U1 8 U2 93 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0044-7447 EI 1654-7209 J9 AMBIO JI Ambio PD MAR PY 2015 VL 44 SU 2 SI SI BP S163 EP S179 DI 10.1007/s13280-014-0612-x PG 17 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CB1TT UT WOS:000349411600001 PM 25681975 ER PT J AU Smith, DR Francesconi, W Livingston, SJ Huang, CH AF Smith, Douglas R. Francesconi, Wendy Livingston, Stan J. Huang, Chi-hua TI Phosphorus losses from monitored fields with conservation practices in the Lake Erie Basin, USA SO AMBIO LA English DT Article; Proceedings Paper CT 7th International Phosphorus Workshop (IPW) CY SEP, 2013 CL Uppsala, SWEDEN DE Blind inlets; Conservation practices; Crop rotation; Grassed waterway; No-tillage; Water quality ID CLAY SOIL; SURFACE WATERS; TILLAGE; CATCHMENTS; QUALITY; RUNOFF; GRASSLAND; TRANSPORT; DRAINAGE; NITROGEN AB Conservation practices are implemented on farm fields in the USA through Farm Bill programs; however, there is a need for greater verification that these practices provide environmental benefits (e.g., water quality). This study was conducted to assess the impact of Farm Bill eligible conservation practices on soluble P (SP) and total P (TP) losses from four fields that were monitored between 2004 and 2013. No-tillage doubled SP loading compared to rotational tillage (e.g., tilled only before planting corn); however, no-tillage decreased TP loading by 69 % compared to rotational tillage. Similarly, grassed waterways were shown to increase SP loads, but not TP loads. A corn-soybean-wheat-oat rotation reduced SP loads by 85 % and TP loads by 83 % compared to the standard corn-soybean rotation in the region. We can potentially attain TP water quality goals using these Farm Bill practices; however, additional strategies must be employed to meet these goals for SP. C1 [Smith, Douglas R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Francesconi, Wendy; Livingston, Stan J.; Huang, Chi-hua] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47906 USA. RP Smith, DR (reprint author), ARS, USDA, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. EM Douglas.R.Smith@ars.usda.gov; Wendy.Francesconi@ars.usda.gov; Stan.Livingston@ars.usda.gov; Chi-hua.Huang@ars.usda.gov NR 42 TC 15 Z9 15 U1 7 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0044-7447 EI 1654-7209 J9 AMBIO JI Ambio PD MAR PY 2015 VL 44 SU 2 SI SI BP S319 EP S331 DI 10.1007/s13280-014-0624-6 PG 13 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CB1TT UT WOS:000349411600014 PM 25681988 ER PT J AU Yeh, HY Hiett, KL Line, JE AF Yeh, Hung-Yueh Hiett, Kelli L. Line, John E. TI Reactions of chicken sera to recombinant Campylobacter jejuni flagellar proteins SO ARCHIVES OF MICROBIOLOGY LA English DT Article DE Campylobacter jejuni; Flagellar proteins; Food-borne pathogen; Zoonoses; epsilon-Proteobacteria; Campylobacteriosis ID MATERNAL ANTIBODIES; PSEUDOMONAS-AERUGINOSA; PATHOGEN; COLONIZATION; PURIFICATION; MOTILITY; POULTRY; HOST; IDENTIFICATION; ANTIGENICITY AB Campylobacter jejuni is a Gram-negative spiral rod bacterium and is the leading but underreported bacterial food-borne pathogen that causes human campylobacteriosis worldwide. Raw or undercooked poultry products are regarded as a major source for human infection. C. jejuni flagella have been implicated in colonization and adhesion to the mucosal surface of chicken gastrointestinal tracts. Therefore, flagellar proteins would be the excellent targets for further investigation. In this report, we used the recombinant technology to generate a battery of C. jejuni flagellar proteins, which were purified by His tag affinity chromatography and determined antigenic profiles of these recombinant flagellar proteins using sera from chickens older than 6 weeks of age. The immunoblot results demonstrate that each chicken serum reacted to various numbers of recombinant flagellar proteins. Among these recombinant proteins, chicken sera reacted predominantly to the FlgE1, FlgK, FlhF, FliG and FliY proteins. These antibody screening results provide a rationale for further evaluation of these recombinant flagellar proteins as potential vaccines for chickens to improve food safety as well as investigation of host immune response to C. jejuni. C1 [Yeh, Hung-Yueh; Hiett, Kelli L.; Line, John E.] ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. RP Yeh, HY (reprint author), ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service CRIS Project [6612-32000-060-00]; US Poultry & Egg Association Project [679] FX We are grateful to Susan Q. Brooks of Poultry Microbiological Safety Research Unit, Agricultural Research Service, US Department of Agriculture, Athens, GA for the excellent technical support and to Dr. Bruce S. Seal for review of the manuscript. This study was supported by the USDA Agricultural Research Service CRIS Project No. 6612-32000-060-00 and the US Poultry & Egg Association Project No. 679. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 36 TC 4 Z9 4 U1 1 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0302-8933 EI 1432-072X J9 ARCH MICROBIOL JI Arch. Microbiol. PD MAR PY 2015 VL 197 IS 2 BP 353 EP 358 DI 10.1007/s00203-014-1062-3 PG 6 WC Microbiology SC Microbiology GA CB1MI UT WOS:000349391400021 PM 25413671 ER PT J AU Su, YK Willis, LB Jeffries, TW AF Su, Yi-Kai Willis, Laura B. Jeffries, Thomas W. TI Effects of Aeration on Growth, Ethanol and Polyol Accumulation by Spathaspora passalidarum NRRL Y-27907 and Scheffersomyces stipitis NRRL Y-7124 SO BIOTECHNOLOGY AND BIOENGINEERING LA English DT Article DE fermentation byproducts; fermentation kinetics; oxygen transfer rate; polyol dehydrogenase; Spathaspora passalidarum; xylose ID RECOMBINANT SACCHAROMYCES-CEREVISIAE; D-ARABITOL DEHYDROGENASE; D-ARABINITOL DEHYDROGENASE; YEAST PICHIA-STIPITIS; D-XYLOSE FERMENTATION; OXYGEN-TRANSFER RATE; CANDIDA-SHEHATAE; ALCOHOLIC FERMENTATION; COENZYME PREFERENCE; ANAEROBIC GROWTH AB Spathaspora passalidarum NN245 (NRRL-Y27907) is an ascomycetous yeast that displays a higher specific fermentation rate with xylose than with glucose. Previous studies have shown that its capacity for xylose fermentation increases while cell yield decreases with decreasing aeration. Aeration optimization plays a crucial role in maximizing bioethanol production from lignocellulosic hydrolysates. Here, we compared the kinetics of S. passalidarum NN245 and Scheffersomyces (Pichia) stipitis NRRL Y-7124 fermenting 15% glucose, 15% xylose, or 12% xylose plus 3% glucose under four different aeration conditions. The maximum specific fermentation rate for S. passalidarum was 0.153g ethanol/g CDWh with a yield of 0.448g/g from 150g/L xylose at an oxygen transfer rate of 2.47mmol O-2/Lh. Increasing the OTR to 4.27mmol O-2/Lh. decreased the ethanol yield from 0.46 to 0.42g/g xylose while increasing volumetric ethanol productivity from 0.52 to 0.8g/Lh. Both yeasts had lower cells yields and higher ethanol yields when growing on xylose than when growing on glucose. Acetic acid accretions of both strains correlated positively with increasing aeration. S. passalidarum secreted lower amounts of polyols compared to S. stipitis under most circumstances. In addition, the composition of polyols differed: S. passalidarum accumulated mostly xylitol and R,R-2,3-butanediol (BD) whereas S. stipitis accumulated mostly xylitol and ribitol when cultivated in xylose or a mixture of 12% xylose and 3% glucose. R,R-2,3-BD accumulation by S. passalidarum during xylose fermentation can be as much as four times of that by S. stipitis, and R,R-2,3-BD is also the most abundant byproduct after xylitol. The ratios of polyols accumulated by the two species under different aeration conditions and the implications of these observations for strain and process engineering are discussed. Biotechnol. Bioeng. 2015;112: 457-469. (c) 2014 Wiley Periodicals, Inc. C1 [Su, Yi-Kai] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53703 USA. [Su, Yi-Kai; Willis, Laura B.; Jeffries, Thomas W.] Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53703 USA. [Willis, Laura B.; Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53703 USA. [Willis, Laura B.; Jeffries, Thomas W.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. RP Jeffries, TW (reprint author), Univ Wisconsin, DOE Great Lakes Bioenergy Res Ctr, Madison, WI 53703 USA. EM twjeffri@wisc.edu OI Jeffries, Thomas/0000-0001-7408-4065 FU Heart of Wisconsin (Wisconsin Rapids); DOE Great Lake Bioenergy Research Center (DOE Office of Science) [BER DE-FC02-07ER64494] FX Contract grant sponsor: Heart of Wisconsin (Wisconsin Rapids); Contract grant sponsor: DOE Great Lake Bioenergy Research Center (DOE Office of Science); Contract grant number: BER DE-FC02-07ER64494 NR 65 TC 5 Z9 5 U1 3 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0006-3592 EI 1097-0290 J9 BIOTECHNOL BIOENG JI Biotechnol. Bioeng. PD MAR PY 2015 VL 112 IS 3 BP 457 EP 469 DI 10.1002/bit.25445 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CA8IJ UT WOS:000349161400004 PM 25164099 ER PT J AU Chung, ST Hsia, DS Chacko, SK Rodriguez, LM Haymond, MW AF Chung, Stephanie T. Hsia, Daniel S. Chacko, Shaji K. Rodriguez, Luisa M. Haymond, Morey W. TI Increased gluconeogenesis in youth with newly diagnosed type 2 diabetes SO DIABETOLOGIA LA English DT Article DE Fasting hyperglycaemia; Gluconeogenesis; Glucose production; Paediatric type 2 diabetes ID HEPATIC GLUCOSE-PRODUCTION; FATTY LIVER-DISEASE; INSULIN SENSITIVITY; POSTPRANDIAL HYPERGLYCEMIA; INDIRECT CALORIMETRY; NONDIABETIC HUMANS; LIPID-METABOLISM; FASTED STATE; MELLITUS; GLYCOGENOLYSIS AB The role of increased gluconeogenesis as an important contributor to fasting hyperglycaemia at diabetes onset is not known. We evaluated the contribution of gluconeogenesis and glycogenolysis to fasting hyperglycaemia in newly diagnosed youths with type 2 diabetes following an overnight fast. Basal rates (mu mol kg(FFM) (-1) min(-1)) of gluconeogenesis ((H2O)-H-2), glycogenolysis and glycerol production ([H-2(5)] glycerol) were measured in 18 adolescents (nine treatment naive diabetic and nine normal-glucose-tolerant obese adolescents). Type 2 diabetes was associated with higher gluconeogenesis (9.2 +/- 0.6 vs 7.0 +/- 0.3 mu mol kg(FFM) (-1) min(-1), p < 0.01), plasma fasting glucose (7.0 +/- 0.6 vs 5.0 +/- 0.2 mmol/l, p = 0.004) and insulin (300 +/- 30 vs 126 +/- 31 pmol/l, p = 0.001). Glucose production and glycogenolysis were similar between the groups (15.4 +/- 0.3 vs 12.4 +/- 1.4 mu mol kg(FFM) (-1) min(-1), p = 0.06; and 6.2 +/- 0.8 vs 5.3 +/- 0.7 mu mol kg(FFM) (-1) min(-1), p = 0.5, respectively). After controlling for differences in adiposity, gluconeogenesis, glycogenolysis and glucose production were higher in diabetic youth (p a parts per thousand currency signaEuro parts per thousand 0.02). Glycerol concentration (84 +/- 6 vs 57 +/- 6 mu mol/l, p = 0.01) and glycerol production (5.0 +/- 0.3 vs 3.6 +/- 0.5 mu mol kg(FFM) (-1) min(-1), p = 0.03) were 40% higher in youth with diabetes. The increased glycerol production could account for only similar to 1/3 of substrate needed for the increased gluconeogenesis in diabetic youth. Increased gluconeogenesis was a major contributor to fasting hyperglycaemia and hepatic insulin resistance in newly diagnosed untreated adolescents and was an early pathological feature of type 2 diabetes. Increased glycerol availability may represent a significant source of new carbon substrates for increased gluconeogenesis but would not account for all the carbons required to sustain the increased rates. C1 [Chung, Stephanie T.] NIDDK, NIH, Bethesda, MD 20892 USA. [Chung, Stephanie T.; Hsia, Daniel S.; Chacko, Shaji K.; Rodriguez, Luisa M.; Haymond, Morey W.] Baylor Coll Med, USDA ARS, Dept Pediat, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Chung, Stephanie T.; Hsia, Daniel S.; Chacko, Shaji K.; Rodriguez, Luisa M.; Haymond, Morey W.] Baylor Coll Med, USDA ARS, Div Pediat Diabet Endocrinol & Metab, Houston, TX 77030 USA. RP Chung, ST (reprint author), NIDDK, NIH, 10 Ctr Dr Bld 10 CRC,RM 5-5740,MSC 1612, Bethesda, MD 20892 USA. EM chungst@niddk.nih.gov FU Intramural NIH HHS [Z99 DK999999]; NIDDK NIH HHS [R01DK 55478, R01 DK055478] NR 50 TC 3 Z9 3 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0012-186X EI 1432-0428 J9 DIABETOLOGIA JI Diabetologia PD MAR PY 2015 VL 58 IS 3 BP 596 EP 603 DI 10.1007/s00125-014-3455-x PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CA9LD UT WOS:000349244100021 PM 25447079 ER PT J AU Cha, DH Hesler, SP Park, S Adams, TB Zack, RS Rogg, H Loeb, GM Landolt, PJ AF Cha, Dong H. Hesler, Stephen P. Park, Shinyoung Adams, Todd B. Zack, Richard S. Rogg, Helmuth Loeb, Gregory M. Landolt, Peter J. TI Simpler is better: fewer non-target insects trapped with a four-component chemical lure vs. a chemically more complex food-type bait for Drosophila suzukii SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE spotted wing drosophila; trap; monitor; attractant; detection; Diptera; Drosophilidae ID ZAPRIONUS-INDIANUS DIPTERA; SYNTHETIC ATTRACTANT; HYMENOPTERA VESPIDAE; FLY DIPTERA; FRUIT; WINE; TEPHRITIDAE; VINEGAR; COMBINATIONS; LEPIDOPTERA AB Baits - fermented food products - are generally attractive to many types of insects, which makes it difficult to sort through non-target insects to monitor a pest species of interest. We test the hypothesis that a chemically simpler and more defined attractant developed for a target insect is more specific and attracts fewer non-target insects than a chemically more complex food-type bait. A four-component chemical lure isolated from a food bait and optimized for the spotted wing drosophila (SWD), Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), was compared to the original wine/vinegar bait to assess the relative responses of non-target insects. In several field experiments in Washington State, USA, it was shown that numbers of pest muscid flies, cutworm and armyworm moths, and pest yellowjackets were reduced in traps baited with the chemical lure compared to the wine/vinegar bait. In other field experiments in the states of Washington, Oregon, and New York, numbers of non-target drosophilid flies were also reduced in traps baited with the chemical lure relative to wine/vinegar bait. In Washington, numbers of Drosophila melanogaster Meigen and Drosophila obscura Fallen species groups and Drosophila immigrans Sturtevant were reduced in the chemical lure traps, whereas in New York, D.melanogaster and D.obscura species groups, D.immigrans, Drosophila putrida Sturtevant, Drosophila simulans Sturtevant, Drosophila tripunctata Loew, and Chymomyza spp. numbers were reduced. In Oregon, this same effect was observed with the D.melanogaster species group. Taken together, these results indicate that the four-component SWD chemical lure will be more selective for SWD compared to fermentation baits, which should reduce time and cost involved in trapping in order to monitor SWD. C1 [Cha, Dong H.; Landolt, Peter J.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Hesler, Stephen P.; Park, Shinyoung; Loeb, Gregory M.] Cornell Univ, Dept Entomol, Geneva, NY USA. [Adams, Todd B.; Rogg, Helmuth] Oregon Dept Agr, Salem, OR USA. [Zack, Richard S.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. RP Cha, DH (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM dongho.cha1@gmail.com FU Washington Tree Fruit Research Commission; Cornell University's New York State Agricultural Experiment Station Federal Formula funds [2012-13-195]; NYS Dept. of Agriculture & Markets Specialty Crops Block Grant [C200783] FX We thank John Jaenike at the University of Rochester (NY, USA) for help with drosophilid identifications and Jewel Brumley, Daryl Green, and Lee Ream for technical assistance. Seth Davis, Wee Yee, and two anonymous reviewers provided suggestions for improvements to the manuscript. This research was supported in part by funding from the Washington Tree Fruit Research Commission to PL, Cornell University's New York State Agricultural Experiment Station Federal Formula funds, project # 2012-13-195 and NYS Dept. of Agriculture & Markets Specialty Crops Block Grant #C200783 to GL. NR 40 TC 7 Z9 7 U1 3 U2 58 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAR PY 2015 VL 154 IS 3 BP 251 EP 260 DI 10.1111/eea.12276 PG 10 WC Entomology SC Entomology GA CB4VG UT WOS:000349625800009 ER PT J AU Ogugo, V Semagn, K Beyene, Y Runo, S Olsen, M Warburton, ML AF Ogugo, Veronica Semagn, Kassa Beyene, Yoseph Runo, Steven Olsen, Michael Warburton, Marilyn L. TI Parental genome contribution in maize DH lines derived from six backcross populations using genotyping by sequencing SO EUPHYTICA LA English DT Article DE Donor parent; Doubled haploid; Drought; GBS; Introgression; Water-stress ID DOUBLED-HAPLOID LINES; TESTCROSS PERFORMANCE; BIPARENTAL CROSSES; GRAPHICAL GENOTYPES; DROUGHT TOLERANCE; HOMOZYGOUS LINES; ORYZA-GLABERRIMA; INBRED LINES; SSR DATA; RICE AB Molecular characterization of doubled haploid (DH) maize lines and estimation of parental genome contribution (PGC) may be useful for choosing pairs of DH lines for hybrid make up and new pedigree starts. Six BC1-derived DH populations created by crossing two donor with three recurrent parents were genotyped with 97,190 polymorphic markers with the objectives of: (i) understanding genetic purity, genetic distance and relationship among 417 maize DH lines; (ii) estimating PGC of the DH lines derived from different genetic backgrounds; and (iii) understanding the correlation between donor parent introgression and testcross performance for grain yield and anthesis-silking interval (ASI) under managed drought and optimum environments. The DH lines were 97 % genetically pure, with < 2 % heterogeneity; only two DH lines showed heterogeneity > 5 %, which is likely to be due to errors during seed multiplication or maintenance. Genetic distance between pairwise comparisons of the 417 DH lines ranged from 0.055 to 0.457; only 0.01 % showed a genetic distance < 0.100, indicating large genetic differences among the DH lines. Both populations 1 and 6 showed significantly lower (p < 0.001) donor introgression than the other four populations. Donor parent contribution was significantly (p < 0.001) higher in the CML444 genetic background than CML395 and CML488. The average donor and recurrent PGC across all 417 DH lines was 31.7 and 64.3 %, respectively. Donor genome introgression was higher than expected in 82 % of the DH lines in the BC1 generation, possibly due to artificial selection during the DH process, during the development of F-1 or BC1 seed, or during initial agronomic evaluation of the DH lines. Donor parent introgression up to 32 % showed significant positive correlation with grain yield under drought (r = 0.312, p < 0.001) and optimum (r = 0.142, p < 0.050) environments but negative correlation with ASI under drought (r = -0.276, p < 0.001). Additional multi-environment phenotype data under managed drought are needed to confirm the correlations reported in this study and to map the specific genomic regions associated with such correlations. C1 [Ogugo, Veronica; Semagn, Kassa; Beyene, Yoseph] Int Maize & Wheat Improvement Ctr CIMMYT, Nairobi 00621, Kenya. [Runo, Steven] Kenyatta Univ, Dept Biochem & Biotechnol, Nairobi 0100, Kenya. [Olsen, Michael] Int Maize & Wheat Improvement Ctr CIMMYT, Mexico City 06600, DF, Mexico. [Warburton, Marilyn L.] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi, MS 39762 USA. RP Semagn, K (reprint author), Int Maize & Wheat Improvement Ctr CIMMYT, POB 1041, Nairobi 00621, Kenya. EM k.semagn@cgiar.org OI Semagn, Kassa/0000-0001-6486-5685 FU Bill & Melinda Gates Foundation; US National Science Foundation FX DH lines used in this study and their phenotypic data were generated as part of the WEMA project, funded by the Bill & Melinda Gates Foundation. GBS data were generated as part of the Basic Research to Enabling Agricultural Development (BREAD) project, funded by the US National Science Foundation. We are grateful to CIMMYT Field Technicians at the different stations in Kenya for the phenotypic evaluation, and Monsanto Company for developing the DH populations. NR 35 TC 1 Z9 1 U1 2 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAR PY 2015 VL 202 IS 1 BP 129 EP 139 DI 10.1007/s10681-014-1238-6 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA CB0SB UT WOS:000349336000011 ER PT J AU Jong, L AF Jong, Lei TI Influence of protein hydrolysis on the mechanical properties of natural rubber composites reinforced with soy protein particles SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Natural rubber; Mechanical properties; Soy protein; Fillers; Hydrolysis ID EMULSIFYING PROPERTIES; FILLER INTERACTIONS; LATEX; NANOCRYSTALS; ISOLATE AB For natural rubber applications, the reinforcing fillers are used to improve the mechanical properties of the rubber. Soy protein particles have been shown to reinforce natural rubber. The hydrolysis conditions of soy protein are studied to understand its effect on the particle size and size distribution, and their subsequent effect on the mechanical properties of natural rubber. The reinforced and crosslinked rubber composites showed an improvement on modulus, tensile strength, and toughness compared to natural rubber. The composites reinforced with less hydrolyzed soy protein and the composites prepared under alkali condition had greater tensile strength, modulus, and toughness, while the composites reinforced with highly hydrolyzed soy protein and the composites prepared under acidic condition had greater elongation. The highly hydrolyzed soy protein was found to decrease the total crosslinking density and decrease the modulus at larger strain region. The structure-properties of the composites were Characterized with stress-strain, dynamic frequency sweep, transmission electron Microscopy, and swelling experiments. Published by Elsevier B.V. C1 USDA ARS, Natl Ctr Agr Utilizat Res, Dept Agr, Peoria, IL 61604 USA. RP Jong, L (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Dept Agr, 1815 N Univ St, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 28 TC 5 Z9 5 U1 2 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2015 VL 65 BP 102 EP 109 DI 10.1016/j.indcrop.2014.12.004 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA CB2AV UT WOS:000349430000014 ER PT J AU Pothula, AK Igathinathane, C Shen, JC Nichols, K Archer, D AF Pothula, Anand Kumar Igathinathane, C. Shen, Jiacheng Nichols, K. Archer, D. TI Milled industrial beet color kinetics and total soluble solid contents by image analysis SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Browning index; Color calibration; Feedstock; Mathematical model; MATLAB; Sugar beet ID COMPUTER VISION SYSTEM; DEGRADATION KINETICS; POTATO-CHIPS; HUMIDITY; STORAGE; SLICES; SHRIMP; MEAT; L. AB Industrial beets are an emerging feedstock for biofuel and bioproducts industry in the US. Milling of industrial beets is the primary step in front end processing for ethanol production. Milled beets undergo color change during juice extraction. A custom designed computer vision system for measurement of milled beet color kinetics, consisting of a digital camera, custom-designed non-reflective enclosure, and color calibration method was developed in the present study. Beet samples of five different total soluble solids (TSS) contents were prepared by washing with cold water for color kinetics measurement. An artificial neural network model was used for converting the red, green, and blue (RGB) values of the acquired sample images to L* a* b* values. Seven color parameters (L*, a*, b*, hue, chroma, browning index (BI), and total color change (Delta E)) were analyzed. Page, user-defined polynomial, and fractional conversion models gave better fits for the experimental data with color parameters than the zeroth order, first order, exponential, and Peleg kinetic models. Of the color parameters studied, L* (Page R-2 > 0.99, user-defined polynomial R-2 > 0.97, and fractional R-2 > 0.93) and Delta E (Page R-2 > 0.98, user-defined polynomial R-2 > 0.94, and fractional R-2 > 0.85) gave the best description for the color change kinetics of milled beets. Developed TSS prediction models from the color measurements based on Page model constant, k(p) with color parameter, L* gave good prediction (R-2 = 0.99), also did the simple linear model based on direct color values (R-2 = 0.98). Measurement and mathematical modeling of milled industrial beets color kinetics will serve as important quality assessment tool in processing the beets for various renewable fuel and products applications. Published by Elsevier B.V. C1 [Pothula, Anand Kumar; Igathinathane, C.; Shen, Jiacheng] N Dakota State Univ, Dept Agr & Biosyst Engn, Fargo, ND 58102 USA. [Nichols, K.] Rodale Inst, Kutztown, PA 19530 USA. [Archer, D.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. RP Igathinathane, C (reprint author), N Dakota State Univ, Dept Agr & Biosyst Engn, 1221 Albrecht Blvd, Fargo, ND 58102 USA. EM Igathinathane.Cannayen@ndsu.edu OI Pothula, Anand/0000-0002-6129-5194; Archer, David/0000-0002-4816-7040; Cannayen, Igathinathane/0000-0001-8884-7959 FU North Dakota Industrial Commission, Bismarck, ND [R-013-025, FAR0020563]; Green Vision Group, Fargo, ND FX Research funding provided by North Dakota Industrial Commission, Bismarck, ND under Renewable Energy Program (R-013-025, 'Energy Beet Research Phase II', Project No. FAR0020563) and Green Vision Group, Fargo, ND are highly appreciated. NR 25 TC 1 Z9 1 U1 4 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2015 VL 65 BP 159 EP 169 DI 10.1016/j.indcrop.2014.12.001 PG 11 WC Agricultural Engineering; Agronomy SC Agriculture GA CB2AV UT WOS:000349430000021 ER PT J AU Sun, Y Veerabomma, S Fokar, M Abidi, N Hequet, E Payton, P Allen, RD AF Sun, Yan Veerabomma, Suresh Fokar, Mohamed Abidi, Noureddine Hequet, Eric Payton, Paxton Allen, Randy D. TI Brassinosteroid signaling affects secondary cell wall deposition in cotton fibers SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Brassinosteroid; BRI1; Cellulose synthase; Cotton fiber; Gossypium hirsutum; Secondary cell wall ID PLANT-GROWTH SUBSTANCES; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; REGULATED GENE; RECEPTOR; ELONGATION; OVULES; KINASE; AUXIN; BIOSYNTHESIS AB Differentiation of cotton fibers involves sequential cell elongation and secondary cell wall deposition as they develop from fiber initials to highly elongated and thickened trichomes on the seed integument. Phytohormones appear to play important regulatory roles in the development of these economically important tells. Previous pharmacological experiments indicate that brassinosteroid (BR), along with auxin and gibberellins, promote cotton fiber elongation. To further evaluate the role of the BR signaling pathway M cotton fiber development, transgenic cotton plants that express transgenes designed to either over-express or suppress the expression of the BR receptor BRI1 were created. Analysis of the cotton fibers from these plants indicated that alteration of BRI1 expression had little effect on fiber length. However, over-expression of BAD led to increased fiber cellulose deposition while suppression of BRI1 expression strongly inhibited secondary cell wall development, resulting in fibers with reduced maturity. These alterations in fiber cell wall development corresponded with changes in cellulose synthase gene expression, indicating that secondary wall deposition is affected by BR-dependent modulation of cellulose synthase gene expression. These results indicate that BR signaling promotes the maturation of cotton fibers through the deposition of cellulose into the secondary cell wall. (C) 2014 Elsevier B.V. All rights reserved. C1 [Sun, Yan; Veerabomma, Suresh] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. [Fokar, Mohamed; Allen, Randy D.] Oklahoma State Univ, Inst Agr Biosci, Ardmore, OK 73401 USA. [Abidi, Noureddine; Hequet, Eric] Texas Tech Univ, Fiber & Biopolymer Res Inst, Lubbock, TX 79409 USA. [Payton, Paxton] USDA ARS, Cropping Syst Res Lab, Lubbock, TX 79415 USA. RP Allen, RD (reprint author), Oklahoma State Univ, Inst Agr Biosci, Ardmore, OK 73401 USA. EM randy.allen@okstate.edu RI Hequet, Eric/A-9802-2008 FU USDA NRI CSREES [2003-35304-13384]; Oklahoma Agricultural Experiment Station [OKL02714] FX The authors wish to acknowledge the generous gift of Brz2001 from Dr. Tadao Asami, and Dr. Shigeo Yoshida, RIKEN, Japan. We also thank Dr. Joanne Chory, Salk Institute for Biological Studies, La Jolla, CA for the anti AtBRI1 antibody. This project was supported by USDA NRI CSREES grant number 2003-35304-13384 and Oklahoma Agricultural Experiment Station project number OKL02714. DNA sequencing was performed at the Texas Tech University Center for Biotechnology and Genomics. NR 46 TC 1 Z9 2 U1 3 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2015 VL 65 BP 334 EP 342 DI 10.1016/j.indcrop.2014.11.028 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA CB2AV UT WOS:000349430000042 ER PT J AU Vaughn, SF Eller, FJ Evangelista, RL Moser, BR Lee, E Wagner, RE Peterson, SC AF Vaughn, Steven F. Eller, Fred J. Evangelista, Roque L. Moser, Bryan R. Lee, Edward Wagner, Richard E. Peterson, Steven C. TI Evaluation of biochar-anaerobic potato digestate mixtures as renewable components of horticultural potting media SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Biochar; Anaerobic digestate; Container production; Potting medium ID DAIRY MANURE; GROWTH; CARBON; PEAT; QUALITY; WASTES; SOIL; VERMICOMPOST; SUBSTITUTE; SUBSTRATE AB Various formulations are used in horticultural potting media, with sphagnum peat moss, vermiculite and perlite currently among the most common components. We are examining a dried anaerobic digestate remaining after the fermentation of potato processing wastes to replace organic components such as peat moss, and biochars produced from several feedstocks as replacements for inorganic media components such as vermiculite and perlite. Biochars were prepared using a top-lit updraft pyrolytic stove from wood pellets, pelletized wheat straw and field pennycress presscake. Biochar yields and heats of combustion were highest with the field pennycress presscake and lowest with the wood pellets. Because all three biochars had basic pHs, they were treated with citric acid solutions to lower pH values to 6.0 before being used in plant experiments. All three acidified biochars were combined in 1:1 ratios with the digestate and compared against a 1:1 sphagnum peat moss:vermiculite control substrate containing slow-release chemical fertilizers. All digestate:biochar substrates had higher bulk densities and levels of soluble salts than the control. Greenhouse experiments were conducted using tomato (Solanum lycopersicum L.) and marigold (Tagetes erecta L.) plants grown in 2.5-L pots. Combining potato anaerobic digestate with acidified wood pellet biochar resulted in increased growth of tomato plants as compared to the peat:vermiculite control, while the digestate:acidified wheat straw pellet biochar substrate was equal to the control for marigold growth. Plants of both species grown in the digestate:pennycress presscake biochar substrate had less growth than the control. From these results it appears that both the digestate:acidified wood pellet biochar and the digestate:acidified straw pellet biochar would likely be acceptable alternatives to peat:vermiculite substrates, and would also be an option for certified organic producers. Published by Elsevier B.V. C1 [Vaughn, Steven F.; Eller, Fred J.; Evangelista, Roque L.; Moser, Bryan R.; Peterson, Steven C.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Lee, Edward] Hydrostraw LLC, Manteno, IL 60950 USA. [Wagner, Richard E.] Microbial Energy Syst Inc, Bloomington, IN 47401 USA. RP Vaughn, SF (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Steven.Vaughn@ars.usda.gov NR 46 TC 5 Z9 5 U1 5 U2 56 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2015 VL 65 BP 467 EP 471 DI 10.1016/j.indcrop.2014.10.040 PG 5 WC Agricultural Engineering; Agronomy SC Agriculture GA CB2AV UT WOS:000349430000060 ER PT J AU Offeman, RD Dao, GT Holtman, KM Orts, WJ AF Offeman, Richard D. Dao, Gloria T. Holtman, Kevin M. Orts, William J. TI Leaching behavior of water-soluble carbohydrates from almond hulls SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Almond hulls; Carbohydrates; Sugars; Sugar alcohols; Countercurrent extraction; Leaching AB Over 58% of the dry matter content of the hulls from the commercial almond (Prunus dulcis (Miller) D.A. Webb) is soluble in warm water (50-70 degrees C) extraction. The water-soluble extractables include useful amounts of fermentable sugars (glucose, fructose, sucrose), sugar alcohols (inositol and sorbitol), polysaccharides, and other components. Extraction rate data were taken over a range of temperatures and particle sizes (including whole hulls). Equilibrium concentrations and liquid retention data were taken over a wide solubles concentration range, enabling use of a standard leaching model for the calculation of stage-to-stage concentrations, and flows in an ideal countercurrent extractor. The relations between recovery, product concentration, number of ideal stages, and ratio of solvent-to-feed were determined using the model. The data indicate that a solutes concentration of 18-20% dry matter in the concentrate liquid is attainable. Due to the nature of the native hulls to preferentially absorb water from dilute solutions, initial contact of hulls with the overhead liquid concentrate (if well below similar to 18% solutes) may result in a significant enhancement of overhead concentration. Published by Elsevier B.V. C1 [Offeman, Richard D.; Dao, Gloria T.; Holtman, Kevin M.; Orts, William J.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Offeman, RD (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM Richard.Offeman@ars.usda.gov FU Trust Fund [58-5325-2-140] FX We thank the Almond Hullers and Processors Association for collecting the almond hull samples, and for financial support under Trust Fund Cooperative Agreement 58-5325-2-140. NR 11 TC 1 Z9 1 U1 2 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAR PY 2015 VL 65 BP 488 EP 495 DI 10.1016/j.indcrop.2014.10.036 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA CB2AV UT WOS:000349430000063 ER PT J AU Venuto, M Garcia, K Halbrook, B AF Venuto, Margaret Garcia, Kristin Halbrook, Brenda TI Analyses of the Contributing Factors Associated With Foodborne Outbreaks in School Settings (2000-2010) SO JOURNAL OF ENVIRONMENTAL HEALTH LA English DT Article ID UNITED-STATES; FOOD SAFETY; DISEASE; SURVEILLANCE AB State-reported school foodborne outbreaks account for about 3.8% (n = 464) of all outbreaks and 8.2% (n = 20,667) of all illnesses reported to the Centers for Disease Control and Prevention's Foodborne Disease Outbreak Surveillance System. Of 464 school foodborne outbreaks, 122 (26%) outbreaks, 7,603 illnesses, and 301 reported food safety errors met the criteria for inclusion in the analyses. The purpose of the authors' study was to examine the role of contributing factors in school foodborne outbreaks. Contamination factors accounted for the greatest proportion (49.2%) of outbreaks involving some level of food handling interaction by a school food service worker, followed by proliferation (34.9%) and survival factors (15.9%). Over 56% of all illnesses were associated with norovirus and food service worker practices. The results of these analyses highlight the importance of effective food safety education programs that focus on the role of contributing factors and prevention of foodborne disease from food safety errors. C1 [Venuto, Margaret] Ctr Dis Control & Prevent, Div State & Local Readiness, Off Publ Hlth Preparedness & Response, Atlanta, GA 30333 USA. [Garcia, Kristin; Halbrook, Brenda] Food & Nutr Serv, Off Food Safety, USDA, Washington, DC USA. RP Venuto, M (reprint author), Ctr Dis Control & Prevent, Div State & Local Readiness, Off Publ Hlth Preparedness & Response, Atlanta, GA 30333 USA. EM yku9@cdc.gov NR 19 TC 1 Z9 1 U1 3 U2 9 PU NATL ENVIRON HEALTH ASSOC PI DENVER PA 720 S COLORADO BLVD SUITE 970, SOUTH TOWER, DENVER, CO 80246 USA SN 0022-0892 J9 J ENVIRON HEALTH JI J. Environ. Health PD MAR PY 2015 VL 77 IS 7 BP 16 EP 20 PG 5 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA CB5IN UT WOS:000349660800004 PM 25796698 ER PT J AU Boateng, AA Serapiglia, MJ Mullen, CA Dien, BS Hashem, FM Dadson, RB AF Boateng, Akwasi A. Serapiglia, Michelle J. Mullen, Charles A. Dien, Bruce S. Hashem, Fawzy M. Dadson, Robert B. TI Bioenergy crops grown for hyperaccumulation of phosphorous in the Delmarva Peninsula and their biofuels potential SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Bioenergy; Phytoremediation; Poultry manure; Potassium; Pyrolysis ID PYROLYSIS PRODUCTS; SOIL-PHOSPHORUS; POULTRY LITTER; POTASSIUM; BIOMASS; PRETREATMENT; SWITCHGRASS; FEEDSTOCK; NITROGEN; GRASSES AB Herbaceous bioenergy crops, including sorghum, switchgrass, and miscanthus, were evaluated for their potential as phytoremediators for the uptake of phosphorus in the Delmarva Peninsula and their subsequent conversion to biofuel intermediates (bin-oil) by fast pyrolysis using pyrolysis-gas chromatography/mass spectroscopy. Four cultivars of sorghum, five cultivars of switchgrass and one miscanthus (Miscanthus x giganteus) were grown in soils with two different levels of poultry manure (PM) applications. Little variation was seen in phosphorus uptake in the two different soils indicating that the levels of available phosphorus in the soil already saturated the uptake ability of the plants. However, all plants regardless of trial took up more phosphorus than that measured for the non- PM treated control. Sorghum accumulated greater levels of nutrients including phosphorus and potassium compared to switchgrass and miscanthus. The levels of these nutrients in the biomass did not have an effect on carbohydrate contents. However, the potential yield and composition of bio-oil from fast pyrolysis were affected by both agronomics and differences in mineral concentrations. Published by Elsevier Ltd. C1 [Boateng, Akwasi A.; Serapiglia, Michelle J.; Mullen, Charles A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Dien, Bruce S.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Hashem, Fawzy M.; Dadson, Robert B.] Univ Maryland Eastern Shores, Dept Agr Food & Resource Sci, Princess Anne, MD 21853 USA. RP Boateng, AA (reprint author), ARS, Sustainable Biofuels & Coprod Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov OI Dien, Bruce/0000-0003-3863-6664 FU USDA National Institute of Food and Agriculture (NIFA) [2010-38821-21580]; Agriculture and Food Research Initiative Competitive grant from the USDA-NIFA [2011 68005-30411] FX This research was funded by the USDA National Institute of Food and Agriculture (NIFA) grant # 2010-38821-21580. The authors would like to thank Jonathan Martin for his expertise and technical assistance on the analytical pyrolysis in this project. The compositional biomass analysis was conducted by Victoria Nguyen, who is supported by Agriculture and Food Research Initiative Competitive grant from the USDA-NIFA (# 2011 68005-30411). NR 27 TC 4 Z9 4 U1 3 U2 25 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD MAR 1 PY 2015 VL 150 BP 39 EP 47 DI 10.1016/j.jenvman.2014.10.030 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CB3BX UT WOS:000349504300005 PM 25460422 ER PT J AU Withrow, JR Smith, EL Koch, FH Yemshanov, D AF Withrow, J. R. Smith, E. L. Koch, F. H. Yemshanov, D. TI Managing outbreaks of invasive species - A new method to prioritize preemptive quarantine efforts across large geographic regions SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Emerald ash borer; Risk assessment; Cost-benefit analysis; Quarantine; Pest management; Invasive species ID EMERALD ASH BORER; AGRILUS-PLANIPENNIS; SENSITIVITY-ANALYSIS; NORTH-AMERICA; RISK ANALYSIS; COLEOPTERA BUPRESTIDAE; INVADING ORGANISMS; SPREAD; UNCERTAINTY; MANAGEMENT AB In pest risk assessment it is frequently necessary to make time-critical decisions regarding management of expanding pest populations. When an invasive pest outbreak is expanding rapidly, preemptive quarantine of areas that are under imminent threat of infestation is one of only a few available management tools that can be implemented quickly to help control the expansion. The preemptive quarantine of locations that surround an infested area also acts as a safeguard to counteract the risk of failed detections of the pest in field surveys. In this paper, we present a method that assesses the suitability of preemptive quarantine measures at the level of small geographical subdivisions (U.S. counties). The cost of a preemptive quarantine in a given county is weighed against the protective benefit of delaying the spread of an outbreak to other neighboring counties. We demonstrate the approach with a decision support model that estimates the suitability of preemptive quarantine across multiple counties that surround areas infested with the emerald ash borer (Agrilus planipennis Fairmaire (EAB), Coleoptera: Buprestidae), an emerging major threat to ash tree species (Fraxinus spp.) in North America. The model identifies the U.S. counties where the installation of preemptive quarantine would most effectively slow the spread of EAB populations and reduce risk to high-value areas. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Withrow, J. R.] Softec Solut Inc, USDA FS FHTET, Ft Collins, CO 80526 USA. [Smith, E. L.] USDA FS Forest Hlth Technol Enterprise Team, Ft Collins, CO 80526 USA. [Koch, F. H.] USDA Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, Res Triangle Pk, NC 27709 USA. [Yemshanov, D.] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Marie, ON P6A 2E5, Canada. RP Withrow, JR (reprint author), Softec Solut Inc, USDA FS FHTET, NRRC Bldg A Ste 331,2150 Ctr Ave, Ft Collins, CO 80526 USA. EM johnwithrow@fs.fed.us; elsmith@fs.fed.us; fhkoch@fs.fed.us; dyemshan@nrcan.gc.ca OI Koch, Frank/0000-0002-3750-4507 FU USDA-FS Task Order [AG-7604-D-09-0541] FX We are very grateful to Marla Downing of USDA-FHTET and Paul Chaloux of USDA-APHIS for their continued support of this project. This project was funded under USDA-FS Task Order AG-7604-D-09-0541. NR 63 TC 0 Z9 0 U1 2 U2 47 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD MAR 1 PY 2015 VL 150 BP 367 EP 377 DI 10.1016/j.jenvman.2014.11.001 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA CB3BX UT WOS:000349504300038 PM 25560653 ER PT J AU Johnson, CR Thavarajah, D Thavarajah, P Payne, S Moore, J Ohm, JB AF Johnson, Casey R. Thavarajah, Dil Thavarajah, Pushparajah Payne, Scott Moore, Jayma Ohm, Jae-Bom TI Processing, cooking, and cooling affect prebiotic concentrations in lentil (Lens culinaris Medikus) SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Lentil; Prebiotics; Raffinose family oligosaccharides; Resistant starch; Food processing; Starch quality; Food composition; Food analysis; Antinutrients ID RESISTANT STARCH FORMATION; IN-VITRO; CHEMICAL-COMPOSITION; LEGUME SEEDS; WHOLE FOOD; DIGESTIBILITY; OLIGOSACCHARIDES; MICROBIOTA; PEA; VARIETIES AB Lentil is an important staple food crop in many regions and is a good source of protein and various micronutrients. Lentil also contains raffinose family oligosaccharides (RFO), resistant starch (RS), and other prebiotic compounds essential for maintenance of healthy gastrointestinal microflora. However, there is a lack of information about concentrations of prebiotics in commercially available, cooked, and processed lentil market classes. This study assessed concentrations of RFO and RS in two commercially available lentil market classes (medium green and small red) and determined changes associated with dehulling, cooking, cooling, and reheating. Mean total RFO concentrations ranged from 5.5 to 6.1% in raw lentils. Total RFO concentration decreased from raw to reheated seeds in two of the four lentil products: whole red (6.1-4.9%) and whole green (5.5-4.3%). Mean RS concentrations in raw, cooked, cooled, and reheated lentil (3.0, 3.0, 5.1, and 5.1% (dry weight), respectively) clearly demonstrate cooling-induced synthesis of RS from gelatinized starch. These results highlight the impact and importance of processing techniques on lentil nutritional quality for both consumer and food industry use. (C) 2014 Elsevier Inc. All rights reserved. C1 [Johnson, Casey R.; Thavarajah, Pushparajah] N Dakota State Univ, Sch Food Syst, Dept 7640, Fargo, ND 58108 USA. [Thavarajah, Dil] Clemson Univ, Sch Agr Forest & Environm Sci, Poole Agr Ctr 270, Clemson, SC 29634 USA. [Payne, Scott; Moore, Jayma] N Dakota State Univ, Electron Microscopy Ctr, NCSL, USDA ARS, Fargo, ND 58102 USA. [Ohm, Jae-Bom] N Dakota State Univ, USDA ARS, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab,Dept 7640, Fargo, ND 58108 USA. RP Thavarajah, D (reprint author), Clemson Univ, Sch Agr Forest & Environm Sci, Poole Agr Ctr 270, Clemson, SC 29634 USA. EM dthavar@clemson.edu FU Northern Pulse Growers Association, ND, USA FX We thank United Pulse Trading, Inc. for providing lentil seed samples. Support for this research was provided by the Northern Pulse Growers Association, ND, USA. NR 46 TC 3 Z9 3 U1 2 U2 24 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD MAR PY 2015 VL 38 BP 106 EP 111 DI 10.1016/j.jfca.2014.10.008 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA CB3AZ UT WOS:000349501500015 ER PT J AU Tangtrakulwanich, K Albuquerque, TA Brewer, GJ Baxendale, FP Zurek, L Miller, DN Taylor, DB Friesen, KA Zhu, JJ AF Tangtrakulwanich, K. Albuquerque, T. A. Brewer, G. J. Baxendale, F. P. Zurek, L. Miller, D. N. Taylor, D. B. Friesen, K. A. Zhu, J. J. TI Behavioural responses of stable flies to cattle manure slurry associated odourants SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Stomoxys calcitrans; attractants; cresol; phenol; traps ID STOMOXYS-CALCITRANS DIPTERA; HOST LOCATION; SPP. DIPTERA; MUSCIDAE; TRAPS; FLY; TSETSE; 1-OCTEN-3-OL; ATTRACTANT; ODORS AB Stable flies (Stomoxys calcitrans [Diptera: Muscidae] L.) are blood-feeding synanthropic pests, which cause significant economic losses in livestock. Stable fly antennae contain olfactory sensilla responsive to host and host environment-associated odours. Field observation indicated that the abundance of stable flies increased significantly in grasslands or crop fields when cattle manure slurry was applied. Major volatile compounds emanating from manure slurry were collected and identified. Behavioural responses of stable flies to those compounds were investigated in laboratory bioassays and field-trapping studies. Results from olfactometer assays revealed that phenol, p-cresol and m-cresol were attractive to adult stable flies. When tested individually, attraction was higher with lower dosages. Stable flies were most attracted to blends of phenol and m-cresol or p-cresol. Traps with binary blend lures caught more stable flies in field trials as well. C1 [Tangtrakulwanich, K.; Brewer, G. J.; Baxendale, F. P.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Albuquerque, T. A.; Zurek, L.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Miller, D. N.; Taylor, D. B.; Friesen, K. A.; Zhu, J. J.] Univ Nebraska, USDA ARS, AMRU, Lincoln, NE 68583 USA. RP Zhu, JJ (reprint author), Univ Nebraska, USDA ARS, AMRU, 305B Entomol Hall, Lincoln, NE 68583 USA. EM Jerry.Zhu@ars.usda.gov RI Taylor, David/G-6025-2014; OI Taylor, David/0000-0002-4378-4867; Miller, Daniel/0000-0003-3476-487X FU USDA [2010-34103-20960, 2012-34381-20191]; Multi-State Research Program [S1030] FX We express our gratitude to Brad Voelker, Dennis Berkebile and Brian Mills for their technical help with this study. Authors would like to thank funding agencies (USDA grant #2010-34103-20960 and #2012-34381-20191) and Multi-State Research Program S1030 for supporting the research. NR 30 TC 2 Z9 2 U1 2 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD MAR PY 2015 VL 29 IS 1 BP 82 EP 87 DI 10.1111/mve.12103 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA CB1MR UT WOS:000349392300010 PM 25557192 ER PT J AU Saenz, D Hall, TL Kwiatkowski, MA AF Saenz, Daniel Hall, Taylor L. Kwiatkowski, Matthew A. TI Effects of urbanization on the occurrence of Batrachochytrium dendrobatidis: do urban environments provide refuge from the amphibian chytrid fungus? SO URBAN ECOSYSTEMS LA English DT Article DE Amphibians; Chytrid fungus; Urbanization; Habitat ID POPULATION DECLINES; INFECTIOUS-DISEASE; FROG ASSEMBLAGES; CHYTRIDIOMYCOSIS; CONSERVATION; AUSTRALIA; ECOLOGY; BIODIVERSITY; PREVALENCE; HABITATS AB Batrachochytrium dendrobatidis (Bd) is a widespread pathogenic fungus that is known to cause the disease, chytridiomycosis, which can be lethal to many amphibians. We compared occurrence rates on spring peepers (Pseudacris crucifer) in urban and forested breeding sites in eastern Texas, USA. All study sites were at approximately the same latitude and altitude, and samples were collected at the same time of year to isolate differences in Bd infection rates between habitat types. We found significant differences (p < 0.001) in the occurrence of Bd between habitats; with dramatically lower rates of occurrence at urban sites (19.5 %), compared to forested sites (62.9 %). The exact reason for the observed differences in the occurrence of Bd is not known, however, we suspect that warmer temperatures or lower population densities and lower species richness at urban sites all could play a role in our results. Our findings suggest that urban environments may provide a refuge for some amphibians from the pathogen. C1 [Saenz, Daniel] US Forest Serv, Southern Res Stn, USDA, Nacogdoches, TX 75965 USA. [Hall, Taylor L.] Angelina Coll, Dept Sci & Math, Lufkin, TX USA. [Kwiatkowski, Matthew A.] Stephen F Austin State Univ, Dept Biol, Nacogdoches, TX 75962 USA. RP Saenz, D (reprint author), US Forest Serv, Southern Res Stn, USDA, 506 Hayter St, Nacogdoches, TX 75965 USA. EM dsaenz@fs.fed.us NR 53 TC 0 Z9 0 U1 8 U2 58 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD MAR PY 2015 VL 18 IS 1 BP 333 EP 340 DI 10.1007/s11252-014-0398-4 PG 8 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA CB1EU UT WOS:000349370500020 ER PT J AU Pornsukarom, S Patchanee, P Erdman, M Cray, PF Wittum, T Lee, J Gebreyes, WA AF Pornsukarom, S. Patchanee, P. Erdman, M. Cray, P. F. Wittum, T. Lee, J. Gebreyes, W. A. TI Comparative Phenotypic and Genotypic Analyses of Salmonella Rissen that Originated from Food Animals in Thailand and United States SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Salmonella Rissen; antimicrobial resistance; PFGE; Thailand; the United States ID ANTIMICROBIAL RESISTANCE; ANTIBIOTIC-RESISTANCE; MOLECULAR CHARACTERIZATION; TROPICAL SEAFOOD; CHICKEN MEAT; SEROVARS; SWINE; PIGS; TYPHIMURIUM; CEFTRIAXONE AB Salmonella enterica serovar Rissen has been recognized as one of the most common serovar among humans and pork production systems in different parts of the world, especially Asia. In the United States, this serovar caused outbreaks but its epidemiologic significance remains unknown. The objectives of this study were to compare the phenotypic (antimicrobial susceptibility) and genotypic attributes of Salmonella Rissen isolated in Thailand (Thai) and the United States (US). All the Thai isolates (n=30) were recovered from swine faecal samples. The US isolates (n=35) were recovered from swine faecal samples (n=29), cattle (n=2), chicken (n=2), dog (n=1) and a ready-to-eat product (n=1). The antimicrobial susceptibility of isolates was determined using the Kirby-Bauer disk diffusion method with a panel of 12 antimicrobials. Pulse-field gel electrophoresis (PFGE) was used to determine the genotypic diversity of isolates. All Thai isolates showed multidrug resistance (MDR) with the most frequent antibiotic resistance shown against ampicillin (100%), sulfisoxazole (96.7%), tetracycline (93.3%), streptomycin (90%) and chloramphenicol (30%). About half of the isolates of USA origin were pan-susceptible and roughly 30% were resistant to only tetracycline (R-type: Te). Salmonella Rissen isolated from Thailand and the USA in this study were found to be clonally unrelated. Genotypic analyses indicated that isolates were clustered primarily based on the geographic origin implying the limited clonality among the strains. Clonal relatedness among different host species within the same geography (USA) was found. We found genotypic similarity in Thai and US isolates in few instances but with no epidemiological link. Further studies to assess propensity for increased inter-regional transmission and dissemination is warranted. C1 [Pornsukarom, S.; Wittum, T.; Gebreyes, W. A.] Ohio State Univ, Dept Vet Prevent Med, Coll Vet Med, Columbus, OH 43210 USA. [Patchanee, P.] Chiang Mai Univ, Fac Vet Med, Dept Food Anim Clin, Chiang Mai 50000, Thailand. [Erdman, M.] USDA, Diagnost Bacteriol Lab, Natl Vet Serv Labs, Ames, IA USA. [Cray, P. F.] ARS, USDA, Athens, GA USA. [Lee, J.] Ohio State Univ, Coll Publ Hlth, Div Environm Hlth Sci, Columbus, OH 43210 USA. [Lee, J.] Ohio State Univ, Coll Food Agr & Environm Sci, Dept Food Sci & Technol, Columbus, OH 43210 USA. RP Gebreyes, WA (reprint author), Ohio State Univ, Dept Vet Prevent Med, 920 Coffey Rd, Columbus, OH 43210 USA. EM gebreyes.1@osu.edu FU National Center For Genetic Engineering and Biotechnology (NSTDA), Thailand [P-10-10409] FX We thank to the National Center For Genetic Engineering and Biotechnology (NSTDA), Thailand (Project ID: P-10-10409) for financial support of the research conducted in Thailand. We are also grateful for the collaboration with the U.S. Department of Agriculture (USDA); National Veterinary Services Laboratories (NVSL) and Bacterial Epidemiology and Antimicrobial Resistance Research (BEAR) for providing isolates for the study. NR 50 TC 3 Z9 3 U1 2 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD MAR PY 2015 VL 62 IS 2 BP 151 EP 158 DI 10.1111/zph.12144 PG 8 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA CB4QC UT WOS:000349611900010 PM 24931512 ER PT J AU Stanley, DW Stanley, TL AF Stanley, David W. Stanley, Terri Lynn TI Untitled SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Editorial Material C1 [Stanley, David W.] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Stanley, DW (reprint author), USDA ARS, Biol Control Insects Res Lab, 1503 S Providence Rd, Columbia, MO 65203 USA. EM stanleyd@missouri.edu; aibpeditorial@centurytel.net NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD MAR PY 2015 VL 88 IS 3 DI 10.1002/arch.21196 PG 1 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA CB0IJ UT WOS:000349308900001 ER PT J AU Zhu, JY Chandra, MS Gu, F Gleisner, R Reiner, R Sessions, J Marrs, G Gao, J Anderson, D AF Zhu, J. Y. Chandra, M. Subhosh Gu, Feng Gleisner, Roland Reiner, Rick Sessions, John Marrs, Gevan Gao, Johnway Anderson, Dwight TI Using sulfite chemistry for robust bioconversion of Douglas-fir forest residue to bioethanol at high titer and lignosulfonate: A pilot-scale evaluation SO BIORESOURCE TECHNOLOGY LA English DT Article DE Sulfite pulping; Pretreatment; High titer biofuel; Forest residues; Lignosulfonate ID ENHANCE ENZYMATIC SACCHARIFICATION; CELLULOSIC ETHANOL-PRODUCTION; LODGEPOLE PINE; DILUTE-ACID; OVERCOME RECALCITRANCE; HIGH SOLIDS; PRETREATMENT; SPORL; LIGNOCELLULOSES; FRACTIONATION AB This study demonstrated at the pilot-scale (50 kg) use of Douglas-fir forest harvest residue, an underutilized forest biomass, for the production of high titer and high yield bioethanol using sulfite chemistry without solid-liquor separation and detoxification. Sulfite Pretreatment to Overcome the Recalcitrance of Lignocelluloses (SPORL) was directly applied to the ground forest harvest residue with no further mechanical size reduction, at a low temperature of 145 degrees C and calcium bisulfite or total SO2 loadings of only 6.5 or 6.6 wt% on oven dry forest residue, respectively. The low temperature pretreatment facilitated high solids fermentation of the un-detoxified pretreated whole slurry. An ethanol yield of 282 L/tonne, equivalent to 70% theoretical, with a titer of 42 g/L was achieved. SPORL solubilized approximately 45% of the wood lignin as directly marketable lignosulfonate with properties equivalent to or better than a commercial lignosulfonate, important to improve the economics of biofuel production. Published by Elsevier Ltd. C1 [Zhu, J. Y.; Chandra, M. Subhosh; Gu, Feng; Gleisner, Roland; Reiner, Rick] USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Chandra, M. Subhosh] Yogi Vemana Univ, Dept Microbiol, Kadapa, India. [Gu, Feng] Nanjing Forestry Univ, Jiangsu Prov Key & Pulp & Paper Sci & Technol, Nanjing, Jiangsu, Peoples R China. [Sessions, John] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Marrs, Gevan; Gao, Johnway; Anderson, Dwight] Weyerhaeuser Co, Federal Way, WA USA. RP Zhu, JY (reprint author), USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. EM jzhu@fs.fed.us FU Agriculture and Food Research Initiative (AFRI) Competitive grant, USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA) [2011-68005-30416]; University Grants Commission (UGC), New Delhi, India FX The authors would like to acknowledge the financial support of the Agriculture and Food Research Initiative (AFRI) Competitive grant (No. 2011-68005-30416), USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA). The authors also thank Novozymes North America for providing the CTec3 enzymes; Dr. Jerry Gargulak of LignoTech for providing the D-748 Lignosulfonate; Fred Matt of USFS-FPL for conducting the chemical composition analysis; Linda Lorenz for assisting measurements of lignosulfonate molecular weight using GPC; Kolby Hirth for analyzing lignosulfonate sulfur content using ICP. Drs. Bruce Dien and Ron Hector of USDA-ARS for providing the YRH-400 strain. Funding was also received from University Grants Commission (UGC), New Delhi, India for the visiting appointment of Dr. Chandra at the USDA Forest Products Lab. NR 31 TC 26 Z9 28 U1 5 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD MAR PY 2015 VL 179 BP 390 EP 397 DI 10.1016/j.biortech.2014.12.052 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AZ2CG UT WOS:000348041600052 PM 25553570 ER PT J AU Pittman, HT Bowerman, WW Grim, LH Grubb, TG Bridges, WC Wierda, MR AF Pittman, H. Tyler Bowerman, William W. Grim, Leland H. Grubb, Teryl G. Bridges, William C. Wierda, Michael R. TI Using nestling plasma to assess long-term spatial and temporal concentrations of organochlorine compounds in bald eagles within Voyageurs National Park, Minnesota, USA SO CHEMOSPHERE LA English DT Article DE PCB; Dieldrin; DDT; Bald eagle; Haliaeetus leucocephalus; Voyageurs National Park ID POLYBROMINATED DIPHENYL ETHERS; PERSISTENT ORGANIC POLLUTANTS; POLYCHLORINATED-BIPHENYLS; ENVIRONMENTAL CONTAMINANTS; GREAT-LAKES; HALIAEETUS-LEUCOCEPHALUS; ATMOSPHERIC DEPOSITION; PCB CONGENERS; BALTIC SEA; PESTICIDES AB The bald eagle (Haliaeetus leucocephalus) population at Voyageurs National Park (VNP) provides an opportunity to assess long-term temporal and spatial trends of persistent environmental contaminants. Nestling bald eagle plasma samples collected from 1997 to 2010 were analyzed for polychlorinated biphenyls (PCBs) and organochlorine pesticides. Trends of total PCBs, total DDTs, 4,4'-DDE, and Dieldrin were analyzed since >50% of nestling plasma samples had detectable concentrations. Total PCBs, total DDTs, and 4,4'-DDE concentrations have all decreased over time (26.09%, 24.09%, and 40.92% respectively). Concentrations of Dieldrin have increased by 50.25%. In this study, 61.1% of all nestlings sampled had detectable concentrations of Dieldrin from all time periods and all areas of VNP. Since Dieldrin is a banned pesticide in North America, the source of this increase is unknown. However, increases and fluctuations in Dieldrin concentration suggest contaminant levels in VNP may be linked to a new source or environmental process. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Pittman, H. Tyler; Bowerman, William W.; Wierda, Michael R.] Clemson Univ, Dept Forestry & Nat Resources, Clemson, SC 29631 USA. [Grim, Leland H.] Voyageurs Natl Pk, Int Falls, MN 56649 USA. [Grubb, Teryl G.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Bridges, William C.] Clemson Univ, Dept Appl Econ & Stat, Clemson, SC 29687 USA. RP Pittman, HT (reprint author), Univ Maryland, Dept Environm Sci & Technol, 1426 Ani Sci Ag Eng Bldg 142, College Pk, MD 20742 USA. EM H.T.Pittman@gmail.com; wbowerma@umd.edu; clgrim57@midco.net; tgrubb@fs.fed.us; wbrdgs@clemson.edu; mike.wierda@gmail.com FU Voyageurs National Park, US Forest Service; Michigan Department of Environmental Quality through the Clean Michigan Fund; Friends of Voyageurs National Park; NIFA/USDA [SC-1700281]; Clemson University Experiment Station [5873] FX This project was supported by Voyageurs National Park, US Forest Service, and the Michigan Department of Environmental Quality through the Clean Michigan Fund, and Friends of Voyageurs National Park. This material is based upon work supported by NIFA/USDA, under project number SC-1700281, Technical Contribution No. 5873 of the Clemson University Experiment Station. NR 58 TC 1 Z9 1 U1 5 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD MAR PY 2015 VL 123 BP 79 EP 86 DI 10.1016/j.chemosphere.2014.12.043 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA CA9VB UT WOS:000349271800011 PM 25563158 ER PT J AU Harrison, HF Farnham, MW Jackson, DM AF Harrison, Howard F., Jr. Farnham, Mark W. Jackson, D. Michael TI Tolerance of broccoli cultivars to pre-transplanting clomazone SO CROP PROTECTION LA English DT Article DE Crop injury; Herbicide tolerance; Cole crops; Weed management ID CHLOROPLAST DEVELOPMENT; BRASSICA-OLERACEA; WEED-CONTROL; HERBICIDE AB Clomazone has been used for weed management in cabbage (Brassica oleracea L, Capitata group) production in the U.S.A. for over 20 years; however, the herbicide is not currently registered for other crop groups within B. oleracea. The specialty crops pesticide registration program (The IR-4 Project) is pursuing clomazone registration for broccoli (B. oleracea, Italica group) and cauliflower (B. oleracea, Botrytis group). The objective of this study was to assess the tolerance of a genetically diverse collection of 44 hybrid broccoli cultivars to pre-transplanting clomazone application and identify susceptible cultivars not suitable for clomazone use. A greenhouse experiment demonstrated differences in tolerance among broccoli cultivars when injury ratings and shoot weights were compared. The most tolerant cultivar, Chief, was only slightly injured and its shoot weight was not reduced in comparison to the untreated controls by clomazone incorporated into potting medium at 3.0 mg/kg; whereas, the most susceptible cultivar, Patron, was moderately injured and its shoot weight was reduced by clomazone at 1.5 mg/kg. Injury ratings from field experiments also demonstrated differences between cultivars in response to pre-transplant clomazone application. At two weeks after transplanting into treated soil, the most tolerant cultivars exhibited minor injury at 1.12 kg/ha, and the most susceptible cultivars were moderately injured by 0.28 kg/ha. Injury ratings were much lower at 6 weeks after transplanting than at two weeks. Broccoli head weights were reduced by 0.28 kg/ha for 4 cultivars in 2012 and 1 cultivar in 2013, and stem diameters of 2 cultivars were reduced by this treatment in 2012. The low rate did not affect harvest date for any cultivar, but at 1.12 kg/ha, clomazone delayed head maturation for twelve cultivars in 2012 and 4 cultivars in 2013. These responses were inconsistent between years, and the greater injury observed in 2012 than in 2013 may have been due to the transplanting method which moved treated soil into the root zone. Overall, the results indicate that pre-transplanting application of clomazone at the rate that is approved for cabbage, 0.28 kg/ha on coarse soils, is relatively safe for use on most of the 44 broccoli cultivars evaluated in this study. The risk of crop injury that may occur under unfavorable environmental conditions can be reduced by choosing the most tolerant cultivars. Published by Elsevier Ltd. C1 [Harrison, Howard F., Jr.; Farnham, Mark W.; Jackson, D. Michael] USDA ARS, US Vegetable Lab, Charleston, SC 29407 USA. RP Harrison, HF (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29407 USA. EM howard.harrison@ars.usda.gov NR 21 TC 0 Z9 0 U1 3 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAR PY 2015 VL 69 BP 28 EP 33 DI 10.1016/j.cropro.2014.11.013 PG 6 WC Agronomy SC Agriculture GA CA9VA UT WOS:000349271700004 ER PT J AU Weaver, MA Abbas, HK Falconer, LL Allen, TW Pringle, HC Sciumbato, GL AF Weaver, Mark A. Abbas, Hamed K. Falconer, Lawrence L. Allen, Tom W. Pringle, H. C. (Lyle), III Sciumbato, Gabe L. TI Biological control of aflatoxin is effective and economical in Mississippi field trials SO CROP PROTECTION LA English DT Article DE Mycotoxin; Aspergillus flavus; Afla-Guard (R); Biopesticide ID ASPERGILLUS-FLAVUS; PLANTING DATE; CORN; CONTAMINATION; ACCUMULATION; INOCULATION; MANAGEMENT; BIOCONTROL; DROUGHT; HYBRIDS AB Aflatoxin contamination of corn reduces grain quality and can negatively impact the economic wellbeing of Mississippi's corn farmers. Mitigation of aflatoxin contamination in corn through pre-harvest field application of non-aflatoxigenic strains of Aspergillus flavus holds promise as an effective management strategy. In this study, field trials were conducted at fourteen sites over three years, in experimental plots and within commercial fields, to evaluate the efficacy of two biocontrol products at 11 or 22 kg ha(-1) application rates and at various timings, including two vegetative-stages (V5 and V10), tasseling (VT), a reproductive application made at blister (R2) and two split-applications. The greatest reduction in aflatoxin generally occurred with applications at the earliest developmental stages. Furthermore, six treatments proved more cost-effective across multiple fields and years. These observations support the use of atoxigenic strains of A. flavus to promote the safety and marketability of corn grown in areas susceptible to aflatoxin contamination. Published by Elsevier Ltd. C1 [Weaver, Mark A.; Abbas, Hamed K.] USDA ARS, Biol Control Pests Res Unit, Natl Biol Control Lab, Stoneville, MS 38776 USA. [Falconer, Lawrence L.; Allen, Tom W.; Pringle, H. C. (Lyle), III; Sciumbato, Gabe L.] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS USA. RP Weaver, MA (reprint author), USDA ARS, Biol Control Pests Res Unit, Natl Biol Control Lab, Stoneville, MS 38776 USA. EM Mark.Weaver@ars.usda.gov OI Weaver, Mark/0000-0001-9020-7264 FU Mississippi Corn Promotion Board FX This work was supported in part by the Mississippi Corn Promotion Board. NR 17 TC 5 Z9 5 U1 2 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAR PY 2015 VL 69 BP 52 EP 55 DI 10.1016/j.cropro.2014.12.009 PG 4 WC Agronomy SC Agriculture GA CA9VA UT WOS:000349271700007 ER PT J AU Fettig, CJ Munson, AS Gibson, KE AF Fettig, Christopher J. Munson, A. Steve Gibson, Kenneth E. TI Alternative timing of carbaryl treatments for protecting lodgepole pine from mortality attributed to mountain pine beetle SO CROP PROTECTION LA English DT Article DE Bole sprays; Dendroctonus ponderosae; Pinus contorta; Tree protection ID DENDROCTONUS-PONDEROSAE; CONIFEROUS FORESTS; ATTACK; COLEOPTERA; BARK; SCOLYTIDAE; TREES; CURCULIONIDAE; PERMETHRIN; STATE AB Carbaryl is regarded among the most effective, economically viable, and ecologically-compatible insecticides available for protecting conifers from bark beetle attack in the western United States. Treatments are typically applied in spring prior to initiation of bark beetle flight for that year. We evaluated the efficacy of spring and fall applications for protecting individual lodgepole pine, Pinus contorta Dougl. ex Loud, from mortality attributed to mountain pine beetle, Dendroctonus ponderosae Hopkins, the most notable forest insect pest in western North America. Both spring and fall treatments of 2.0% a.i. carbaryl (Sevin (R) SL) were efficacious for two field seasons, while results from a third field season were inconclusive due to insufficient beetle pressure. We discuss the implications of these and other results to the management of D. ponderosae. Published by Elsevier Ltd. C1 [Fettig, Christopher J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Munson, A. Steve] US Forest Serv, USDA, Ogden, UT 84403 USA. [Gibson, Kenneth E.] US Forest Serv, USDA, Missoula, MT 59807 USA. RP Fettig, CJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. EM cfettig@fs.fed.us FU USDA Forest Service Pesticide Impact Assessment Program (FS-PIAP) [R1/4-2011-01] FX We thank D. Bennett, R. Cruz, V. DeBlander, G. DeNitto, L Dunning, C. Hayes, J. Neumann, D. Reboletti (Forest Health Protection, USDA Forest Service), C. Dabney and S. McKelvey (Pacific Southwest Research Station, USDA Forest Service), and L. Mortenson (Pacific Northwest Research Station, USDA Forest Service) for technical assistance. In addition, we thank A. Mendonca and H. Whitlach (Bridger-Teton National Forest) for providing access to the study site. Chris Olsen (Bayer Environmental Science) provided the Sevin (R) SL. This research was supported, in part, by a USDA Forest Service Pesticide Impact Assessment Program (FS-PIAP) grant (R1/4-2011-01) to C.J. Fettig, A.S. Munson, and K.E. Gibson. NR 26 TC 0 Z9 0 U1 2 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAR PY 2015 VL 69 BP 56 EP 59 DI 10.1016/j.cropro.2014.12.011 PG 4 WC Agronomy SC Agriculture GA CA9VA UT WOS:000349271700008 ER PT J AU Bredeson, MM Reese, RN Lundgren, JG AF Bredeson, Michael M. Reese, R. Neil Lundgren, Jonathan G. TI The effects of insecticide dose and herbivore density on tri-trophic effects of thiamethoxam in a system involving wheat, aphids, and ladybeetles SO CROP PROTECTION LA English DT Article DE Non-target effects; Clothianidin; Coleomegilla maculata; Predators; Rhophalosiphum padi; Systemic insecticide; Tri-trophic interactions; Wheat ID COLEOMEGILLA-MACULATA COLEOPTERA; NEONICOTINOID SEED TREATMENTS; CHERRY-OAT APHID; RHOPALOSIPHUM-PADI; CHRYSOPERLA-CARNEA; BIOLOGICAL-CONTROL; NATURAL ENEMIES; SOYBEAN APHID; LADY BEETLE; COCCINELLIDAE AB We assess how herbivore density and insecticide dose affects the tri-trophic interactions among thiamethoxam-treated wheat (Triticum sp.), Rhophalosiphum padi and the predatory Coleomegilla maculata DeGeer. In the first experiment 2nd and 4th instar C. maculata were fed aphids reared for 24 h on wheat plants treated with sub-lethal thiamethoxam soil drenches to assess the effect of insecticidal dose on the predator. In the second experiment a constant, sub-lethal thiamethoxam soil drench was used on wheat plants, but the plants were infested with different densities of aphids. Aphids from each density treatment were then fed to C. maculata 2nd instars. Insecticide levels within wheat and aphid tissues were quantified using a competitive ELISA. In experiment one, 4th instars of C. maculata displayed no negative effects after consuming aphids from treated plants. However, 2nd instars of C. maculata ate more treated aphids than untreated aphids and larval development times varied between the untreated larvae and those receiving the low dose of the insecticide. In experiment two, the 2nd instar C. maculata displayed slower walking speeds after consuming aphids from low aphid density plants. Thiamethoxam within wheat tissue was found at higher levels with increasing dose. This insecticide was also found at higher levels in wheat plants with the lowest aphid density. Clothianidin, a toxic metabolite of thiamethoxam, was found in aphids that had fed on thiamethoxam-treated wheat, but no differences between treatments were observed. The neonicotinoid treatment altered the quality of R. padi as prey for 2nd instar C. maculata, but this depended on the aphid infestation level on the plants. In addition to revealing a potential deleterious effect of thiamethoxam systemic insecticides in wheat, our assays provide insight into the design and interpretation of toxicity assays involving systemic neonicotinoids and herbivores. Published by Elsevier Ltd. C1 [Bredeson, Michael M.; Reese, R. Neil] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Bredeson, Michael M.; Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Lundgren, JG (reprint author), USDA ARS, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM jonathan.lundgren@ars.usda.gov NR 50 TC 2 Z9 2 U1 4 U2 52 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAR PY 2015 VL 69 BP 70 EP 76 DI 10.1016/j.cropro.2014.12.010 PG 7 WC Agronomy SC Agriculture GA CA9VA UT WOS:000349271700011 ER PT J AU Wiens, GD Marancik, DP Zwollo, P Kaattari, SL AF Wiens, Gregory D. Marancik, David P. Zwollo, Patty Kaattari, Stephen L. TI Reduction of rainbow trout spleen size by splenectomy does not alter resistance against bacterial cold water disease SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Flavobacterium psychrophilum; Bacterial cold water disease; Rainbow trout; Innate immunity; Splenectomy ID ONCORHYNCHUS-MYKISS; FLAVOBACTERIUM-PSYCHROPHILUM; PLASMA-CELLS; FISH; FEATURES; PERFORMANCE; CHALLENGE; INFECTION; SURVIVAL; QTL AB In lower vertebrates, the contribution of the spleen to anti-bacterial immunity is poorly understood. We have previously reported a phenotypic and genetic correlation between resistance to Flavobacterium psychrophilum, the causative agent of bacterial cold water disease (BCWD) and spleen somatic index (spleen weight normalized to body weight, SI). Fish families with larger pre-challenge SI values were found to have greater BCWD survival (resistance) following intraperitoneal injection of a lethal dose of F. psychrophilum. Since the mammalian spleen is known to be crucial for capture and destruction of encapsulated bacteria, we tested the hypothesis that reduction of spleen size, by surgical splenectomy, should reduce the survival advantage of the larger-spleen, disease-resistant fish. Experiments were performed using two separate lines of fish that had previously been selected either based on BCWD survival (resistant and susceptible), or selected based on spleen size (high and lows!). Following 65 to 81 days post-surgical recovery, fish were challenged with E psychrophilum and mortality monitored for a minimum of 21 days. No significant difference in the relative survival was detected between splenectomized or sham-operated groups, while SI of splenectomized fish was reduced to an average of 8-12% of control animals. A positive correlation was observed between the SI, measured at the time of splenectomy, and time-to-death post-challenge. In summary, these experiments argue that larger spleen size alone is not sufficient for greater BCWD resistance, but rather it is an indirect indicator of immunological status. Published by Elsevier Ltd. C1 [Wiens, Gregory D.; Marancik, David P.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Keameysville, WV 25430 USA. [Zwollo, Patty] Coll William & Mary, Dept Biol, Williamsburg, VA 23185 USA. [Kaattari, Stephen L.] Coll William & Mary, Virginia Inst Marine Sci, Dept Environm & Aquat Anim Hlth, Williamsburg, VA 23185 USA. RP Wiens, GD (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Keameysville, WV 25430 USA. EM greg.wiens@ars.usda.gov OI Wiens, Gregory/0000-0002-5478-7780 FU NIFA FX The authors would like to thank Travis Moreland and Joel Caren for technical expertise. We thank Dr. Tim Leeds for generating Rand S-line fish and help with planning the experimental design. We thank Dr. Brenda Wiens for critical review of this manuscript. This work was supported by NIFA Grant #2012-67015-30217 and Agricultural Research Service CRIS Project 1930-32000-005. The authors have no conflict of interest to declare. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity employer. NR 34 TC 2 Z9 3 U1 0 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD MAR PY 2015 VL 49 IS 1 BP 31 EP 37 DI 10.1016/j.dci.2014.11.003 PG 7 WC Immunology; Zoology SC Immunology; Zoology GA CA4QN UT WOS:000348889800004 PM 25445908 ER PT J AU Yu, LX Shen, X Setter, TL AF Yu, Long-Xi Shen, Xin Setter, Tim L. TI Molecular and functional characterization of two drought-induced zinc finger proteins, ZmZnF1 and ZmZnF2 from maize kernels SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Transcription factor; Effector; Reporter; Gene expression; RNA interference ID STRESS TOLERANCE; ABSCISIC-ACID; EXPRESSION ANALYSIS; GENE FAMILY; RICE; PLANTS; COLD; IDENTIFICATION; TRANSFORMATION; ALEURONE AB Zinc finger proteins are among the most abundant proteins in eukaryotic cells. Their functions are diverse, including DNA and RNA banding, transcriptional activation, regulation of apoptosis, protein folding and assembly. We have isolated two cDNA clones encoding zinc finger proteins, designated as ZmZnF1 and ZmZnF2, from water-stressed maize kernels. Sequence analyses indicates that ZmZnF1 is homologous to the A20/AN1-type zinc finger protein and contains the zinc finger motif of Cx2-Cx10-CxCx4Cx2Hx5HxC, whereas ZmZnF2 is a ring-binding type and contains 3 repeats of the Cx-Cx-H-Cx-Cx zinc finger motif. RNA gel blot analyses indicate that both genes were significantly induced by ABA, osmotic stress (mannitol) and NaCl stress. Using an effector-reporter strategy, we transiently expressed ZmZnE1 or ZmZnF2 with the GUS reporter driven by the rice actin1 promoter containing ABA responsive element, ABRC1 in maize suspension cells. Assay of GUS activity showed that both ZmZnF1 and ZmZnF2 activated the reporter gene expression. A synergistic effect on activation was found between ZmZnE2 and another ABA response factor, VP1. These results were confirmed by the complementary test using RNAi technology. Our results suggest that both ZmZnF1 and ZmZnF2 are transcription activators and may play roles in regulating expression of drought-responsive genes or other transcription factors in response to abiotic stress. Published by Elsevier B.V. C1 [Yu, Long-Xi] ARS, USDA, Vegetable & Forage Crop Res Unit, Prosser, WA 99350 USA. [Shen, Xin; Setter, Tim L.] Cornell Univ, Dept Crop & Soil Sci, Ithaca, NY 14850 USA. RP Yu, LX (reprint author), ARS, USDA, Vegetable & Forage Crop Res Unit, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM longxi.yu@ars.usda.gov NR 28 TC 0 Z9 0 U1 2 U2 27 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD MAR PY 2015 VL 111 BP 13 EP 20 DI 10.1016/j.envexpbot.2014.10.004 PG 8 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA CA4SQ UT WOS:000348895100002 ER PT J AU Zhu, LL Johnson, DA Wang, WG Ma, L Rong, YP AF Zhu, Lingling Johnson, Douglas A. Wang, Weiguang Ma, Lei Rong, Yuping TI Grazing effects on carbon fluxes in a Northern China grassland SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Ecosystem respiration; Daytime ecosystem CO2 exchange; Gross ecosystem production ID LONG-TERM IMPACTS; SOIL CO2 FLUX; DIOXIDE FLUXES; TALLGRASS PRAIRIE; SEMIARID STEPPE; INNER-MONGOLIA; INTERANNUAL VARIABILITY; TEMPERATE GRASSLAND; TIBETAN PLATEAU; GREAT-PLAINS AB Grazing management is critical and can play a significant role in driving C sink and source activity in grassland ecosystems. In this study, CO2 exchange patterns during the growing season were evaluated in the grasslands of northern China using a closed-chamber technique at three stocking rates sites. The results showed that heavy grazing markedly reduced green biomass, plant standing dead, and litter mass as well as reduced net ecosystem exchange (NEE) (P < 0.05). Principal component analysis (PCA) indicated that spatial and temporal patterns varied in ungrazed, moderate grazing, and heavy grazing sites. High NEE was associated with high biomass, high temperature, and high soil water content (SWC), ecosystem respiration (Re), and soil respiration (Rs). Rs was higher at the moderate and heavy grazing sites than the ungrazed site. In contrast, Re and canopy respiration (Rc) were higher at the ungrazed than the other grazing sites. These results indicated that grazing influenced vegetation and soil characteristics, which altered the spatial and temporal patterns of CO2 fluxes in grasslands of northern China. Therefore, reducing stocking rates on heavily grazed grasslands of northern China to moderate grazing levels would enhance NEE, and benefit biomass and animal production. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Zhu, Lingling; Wang, Weiguang; Ma, Lei; Rong, Yuping] China Agr Univ, Anim Sci & Technol Coll, Beijing 100193, Peoples R China. [Zhu, Lingling] Australia Natl Univ, Coll Med Biol & Environm, Canberra, ACT 0200, Australia. [Johnson, Douglas A.] Utah State Univ, USDA ARS, Forage & Range Res Lab, Logan, UT 84322 USA. [Wang, Weiguang] Marshall Univ, Coll Engn, Huntington, WV 25703 USA. RP Rong, YP (reprint author), China Agr Univ, Anim Sci & Technol Coll, Dept Grassland Sci, 2 Yuan Ming Yuan West Rd, Beijing 100193, Peoples R China. EM rongyuping@cau.edu.cn FU State Key Development Program for Basic Research of China [2014CB138805]; National Natural Science Foundation of China [31472136]; China Special Public Sector Research Project [201003019] FX Financial support for this research was provided by the State Key Development Program for Basic Research of China (No. 2014CB138805), the National Natural Science Foundation of China (No. 31472136) and China Special Public Sector Research Project (No. 201003019). We thank F. Yuan, Zh.M. Wang, M. Zhao, and H. Wang for their help with the field work. NR 54 TC 5 Z9 7 U1 9 U2 83 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAR PY 2015 VL 114 BP 41 EP 48 DI 10.1016/j.jaridenv.2014.11.004 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA9VM UT WOS:000349272900005 ER PT J AU Moffet, CA Taylor, JB Booth, DT AF Moffet, Corey A. Taylor, J. Bret Booth, D. Terrance TI Postfire shrub cover dynamics: A 70-year fire chronosequence in mountain big sagebrush communities SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Postfire shrub cover change; Sagebrush; Bitterbrush; Horsebrush; Shrub canopy cover ID SCALE AERIAL IMAGERY; SAGE-GROUSE HABITAT; BITTERBRUSH; RECOVERY; LIVESTOCK; STEPPE; RANGE; DEER AB Fire is natural in sagebrush (Artemisia L.) communities. In this study, we quantify effects of time since last burn (TSLB) on shrub cover over a 70-year (yr) fire chronosequence. We sampled mountain big sagebrush communities with very large-scale aerial (VLSA) imagery and measured sagebrush, antelope bitterbrush (Purshia tridentata [Pursh] DC.), and spineless horsebrush (Tetradymia canescens DC.) cover. We used segmented regression to describe two cover phases with respect to TSLB. Phase 1 was when cover responded to TSLB and Phase 2 was when cover had reached a steady state with respect to TSLB meaning that expected shrub cover did not change with increasing TSLB. In the first year after burning, total shrub cover was 5%. In Phase 1, total shrub, sagebrush, and bitterbrush cover increased with TSLB. Bitterbrush transitioned to Phase 2 in 6 yr, but 19 and 18 yr, respectively, were needed for sagebrush and total shrub to transition. Horsebrush cover decreased with TSLB from 2.1% to 0.2% over 27 yr. Steady-state cover for sagebrush, bitterbrush, and total shrub were 30.6, 2.8, and 39.8%, respectively. These data describe postfire shrub cover change in mountain big sagebrush communities that can be used in management plans that meet shrub cover objectives. (C) 2014 The Authors. Published by Elsevier Ltd. C1 [Moffet, Corey A.] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. [Taylor, J. Bret] USDA ARS, US Sheep Expt Stn, Dubois, ID USA. [Booth, D. Terrance] USDA ARS, High Plains Grasslands Res Stn, Cheyenne, WY USA. RP Moffet, CA (reprint author), Samuel Roberts Noble Fdn Inc, 2510 Sam Noble Pkwy, Ardmore, OK 73401 USA. EM camoffet@noble.org OI Moffet, Corey/0000-0002-7071-7539 NR 32 TC 3 Z9 3 U1 1 U2 16 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD MAR PY 2015 VL 114 BP 116 EP 123 DI 10.1016/j.jaridenv.2014.12.005 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA9VM UT WOS:000349272900016 ER PT J AU Boardman, CR Glass, SV AF Boardman, C. R. Glass, Samuel V. TI Moisture transfer through the membrane of a cross-flow energy recovery ventilator: Measurement and simple data-driven modeling SO JOURNAL OF BUILDING PHYSICS LA English DT Article DE Energy recovery ventilator; moisture transfer effectiveness; indoor air quality; sorption isotherm; moisture transfer model; membrane; permeability; moisture diffusion ID WATER-VAPOR; EXCHANGER; HEAT AB The moisture transfer effectiveness (or latent effectiveness) of a cross-flow, membrane-based energy recovery ventilator is measured and modeled. Analysis of in situ measurements for a full year shows that energy recovery ventilator latent effectiveness increases with increasing average relative humidity and surprisingly increases with decreasing average temperature. A simple finite difference heat and moisture transfer model is developed, which can explain these results and predict energy recovery ventilator latent effectiveness based on simplified physics and material properties. The model parameters are discussed and, in the case of the membrane's moisture sorption curve and moisture permeability, compared to direct laboratory measurements. C1 [Boardman, C. R.; Glass, Samuel V.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Boardman, CR (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM cboardman@fs.fed.us OI Boardman, Charles/0000-0002-9571-5601 FU Forest Products Laboratory (FPL) FX The authors acknowledge the support of the Forest Products Laboratory (FPL) in carrying out this work. NR 24 TC 1 Z9 1 U1 0 U2 8 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1744-2591 EI 1744-2583 J9 J BUILD PHYS JI J. Build Phys. PD MAR PY 2015 VL 38 IS 5 BP 389 EP 418 DI 10.1177/1744259113506072 PG 30 WC Construction & Building Technology SC Construction & Building Technology GA CB0MQ UT WOS:000349320800001 ER PT J AU Zingg, JM Azzi, A Meydani, M AF Zingg, Jean-Marc Azzi, Angelo Meydani, Mohsen TI Induction of VEGF Expression by Alpha-Tocopherol and Alpha-Tocopheryl Phosphate via PI3K gamma/PKB and hTAP1/SEC14L2-Mediated Lipid Exchange SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Article DE VEGF; SEC14-LIKE PROTEINS; TOCOPHEROL; PHOSPHOLIPIDS; VITAMIN E; KINASE; TOCOPHERYL PHOSPHATE; hTAP; SUPERNATANT PROTEIN FACTOR; SPF; PKB; PI3K; PI3K gamma ID ENDOTHELIAL GROWTH-FACTOR; PHOSPHATIDYLINOSITOL TRANSFER PROTEINS; HUMAN SEC14P-LIKE PROTEINS; PREVENTION TRIAL SELECT; VITAMIN-E; PROSTATE-CANCER; CELL-PROLIFERATION; GENE-EXPRESSION; REDUCED EXPRESSION; CORTICAL-NEURONS AB In several studies, vitamin E has been observed to influence angiogenesis and vasculogenesis. We recently showed that the phosphorylated form of alpha-tocopherol (alpha T), alpha-tocopheryl phosphate (alpha TP), increases the expression of the vascular endothelial growth factor (VEGF). Thus, alpha TP may act as an active lipid mediator increasing VEGF expression, angiogenesis, and vasculogenesis. Here, we investigated the molecular signaling mechanisms by which alpha TP induces VEGF expression using cultured HEK293 cells as model system. alpha T and more so alpha TP increased VEGF-promoter activity in a phosphatidylinositol-3-kinase gamma (PI3K gamma)-dependent manner. In contrast, after overexpression of PI3K gamma and/or protein kinase B (PKB), VEGF promoter activity was inhibited by alpha T and more so by alpha TP. Inhibition by alpha T and alpha TP was dependent on the lipid kinase activity of PI3K gamma, whereas an induction was seen with the protein kinase activity, consistent with a model in which PKB inhibition by alpha T or alpha TP occurs only when activated at the plasma membrane and possibly involves a phosphatase such as PHLPP1. PI3K gamma-induced VEGF expression was reduced when the human tocopherol-associated protein 1 (hTAP1/SEC14L2) was overexpressed suggesting formation of an inactive PI3K gamma/hTAP1 heterodimer, that could be reactivated by alpha T and more so by alpha TP. We suggest a novel signaling mechanism by which alpha TP stimulates PI3K gamma activity by stimulating hTAP-mediated phosphatidylinositol exchange and presentation to the enzyme and/or dissociation of an inactive heterodimer. At cellular level, hTAP may act as sensor for intracellular lipid information (location, type, and amount of lipid) and translate it into responses of PI3K-mediated signaling and gene expression. (C) 2014 Wiley Periodicals, Inc. C1 [Zingg, Jean-Marc; Azzi, Angelo; Meydani, Mohsen] Tufts Univ, JM USDA, Human Nutr Res Ctr Aging, Vasc Biol Lab, Boston, MA 02111 USA. RP Zingg, JM (reprint author), Tufts Univ, JM USDA, Human Nutr Res Ctr Aging, Vasc Biol Lab, Off 621,711 Washington St, Boston, MA 02111 USA. EM Jean-Marc.Zingg@tufts.edu FU US Department of Agriculture [58-1950-0-014]; National Institute of Food and Agriculture (NIFA/USDA) [2010-65200-20395] FX Grant sponsor: US Department of Agriculture; Grant number: 58-1950-0-014; Grant sponsor: National Institute of Food and Agriculture (NIFA/USDA); Grant number: 2010-65200-20395. NR 73 TC 6 Z9 6 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-2312 EI 1097-4644 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD MAR PY 2015 VL 116 IS 3 BP 398 EP 407 DI 10.1002/jcb.24988 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA AZ9YG UT WOS:000348569900006 PM 25290554 ER PT J AU Bonan, RF Bonan, PRF Batista, AUD Sampaio, FC Albuquerque, AJR Moraes, MCB Mattoso, LHC Glenn, GM Medeiros, ES Oliveira, JE AF Bonan, Roberta F. Bonan, Paulo R. F. Batista, Andre U. D. Sampaio, Fabio C. Albuquerque, Allan J. R. Moraes, Maria C. B. Mattoso, Luiz H. C. Glenn, Gregory M. Medeiros, Eliton S. Oliveira, Juliano E. TI In vitro antimicrobial activity of solution blow spun poly(lactic acid)/polyvinylpyrrolidone nanofibers loaded with Copaiba (Copaifera sp.) oil SO MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS LA English DT Article DE Nanofibers; Drug delivery system; (Copaifera sp.) oil; Solution blow spinning; Antimicrobial activity ID CONTROLLED DRUG-DELIVERY; MULTIJUGA HAYNE; POLYMER BLENDS; ACID); RELEASE; MEMBRANES; SYSTEMS; FIBERS; NANOPARTICLES; SINGLE AB In this study poly(lactic add) (PLA) and polyvinylpyrrolidone (PVP) micro- and nanofiber mats loaded with Copaiba (Copaifera sp.) oil were produced by solution blow spinning (SBS). The Copaiba (Copaifera sp.) oil was characterized by gas chromatography (GC). Neat PLA and four PLA/PVP blends containing 20% (wt.%) oil were spun and characterized by scanning electron microscopy (SEM) and by studying the surface contact angle, in vitro release rate, and antimicrobial activity. All compositions evaluated were able to produce continuous and smooth fibers by SBS. The addition of PVP increased fiber diameter, and decreased the surface contact angle. GC analysis demonstrated that the main component of the Copaiba oil was beta-caryophyllene, a known antimicrobial agent. In vitro release tests of Copaiba oil volatiles demonstrated a higher release rate in fibers containing PVP. Fiber mats made from blends containing higher amounts of PVP had greater antimicrobial action against Staphylococcus aureus. The results confirm the potential of the fiber mats for use in controlled drug release and could lead to promising applications in the biomedical field. (C) 2014 Elsevier B.V. All rights reserved. C1 [Bonan, Roberta F.; Medeiros, Eliton S.] Univ Fed Paraiba, Dept Engn Mat DEMAT, BR-58051900 Joao Pessoa, Paraiba, Brazil. [Bonan, Roberta F.; Bonan, Paulo R. F.; Batista, Andre U. D.; Sampaio, Fabio C.; Albuquerque, Allan J. R.] Univ Fed Paraiba, Ctr Ciancias & Saude, BR-58051900 Joao Pessoa, Paraiba, Brazil. [Moraes, Maria C. B.] Embrapa Recursos Genet & Biotecnol, Lab Semioquim, ASA NORTE, BR-70770900 Brasilia, DF, Brazil. [Mattoso, Luiz H. C.] Embrapa Instrumentacao Agr CNPDIA, Lab Nacl Nanotecnol & Agronegacio, BR-1356097 Sao Carlos, SP, Brazil. [Glenn, Gregory M.] USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Oliveira, Juliano E.] Univ Fed Lavras, Dept Engn, BR-37200000 Lavras, MG, Brazil. RP Oliveira, JE (reprint author), Univ Fed Lavras, Dept Engn, POB 3037, BR-37200000 Lavras, MG, Brazil. EM juliano.materiais@gmail.com RI Blassioli-Moraes, Maria carolina /C-6072-2013; Albuquerque, Allan/J-5865-2013; Mattoso, Luiz H C/D-2794-2016; Bonan, Paulo /D-6571-2017 OI Blassioli-Moraes, Maria carolina /0000-0001-7569-9985; Albuquerque, Allan/0000-0003-4640-0635; Mattoso, Luiz H C/0000-0001-7586-1014; Bonan, Paulo /0000-0002-4449-4343 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPQ [476362/2012-1, 562.703/2010-4]; Laboratorio de Solidificacao Rapida (LSR) da UFPB FX We gratefully acknowledge the financial support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPQ (Processes 476362/2012-1 and 562.703/2010-4) and Laboratorio de Solidificacao Rapida (LSR) da UFPB. NR 42 TC 8 Z9 8 U1 11 U2 56 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0928-4931 EI 1873-0191 J9 MAT SCI ENG C-MATER JI Mater. Sci. Eng. C-Mater. Biol. Appl. PD MAR 1 PY 2015 VL 48 BP 372 EP 377 DI 10.1016/j.msec.2014.12.021 PG 6 WC Materials Science, Biomaterials SC Materials Science GA CA2PE UT WOS:000348749200048 PM 25579936 ER PT J AU Sicher, R Bunce, J Barnaby, J Bailey, B AF Sicher, R. Bunce, J. Barnaby, J. Bailey, B. TI Water-deficiency effects on single leaf gas exchange and on C-4 pathway enzymes of maize genotypes with differing abiotic stress tolerance SO PHOTOSYNTHETICA LA English DT Article DE C-4 photosynthesis; drought; enzyme activities; gene expression; phosphoenolpyruvate carboxylase ID CARBON-DIOXIDE; METABOLIC LIMITATIONS; DROUGHT STRESS; PHOTOSYNTHESIS; LEAVES; PLANTS; RESPONSES; ASSIMILATION; TEMPERATURE; NITROGEN AB Responses to drought were studied using two maize inbred lines (B76 and B106) and a commercial maize hybrid (Zea mays L. cv. Silver Queen) with differing resistance to abiotic stress. Maize seedlings were grown in pots in controlled environment chambers for 17 days and watering was withheld from one half the plants for an additional 11 days. On the final treatment date, leaf water potentials did not differ among genotypes and were -0.84 and -1.49 MPa in the water sufficient and insufficient treatments, respectively. Greater rates of CO2 assimilation were retained by the stress tolerant maize inbred line, B76, in comparison to the other two genotypes 11 days after watering was withheld. Rates of CO2 assimilation for all three genotypes were unaffected by decreasing the measurement O-2 concentration from 21 to 2% (v/v). Activities of phosphoenolpyruvate carboxylase (PEPC), NADP-malic enzyme (NADP-ME), and NADP malate dehydrogenase were inhibited from 25 to 49% by the water deficiency treatment. Genotypic differences also were detected for the activities of NADP-ME and for PEPC. Changes of transcript abundance for the three C-4 pathway enzymes also varied among watering treatments and genotypes. However, examples where transcripts decreased due to drought were associated with the two stress susceptible genotypes. The above results showed that enzymes in the C-4 photosynthetic pathway were less inhibited by drought in stress tolerant compared to stress susceptible maize genotypes. C1 [Sicher, R.; Bunce, J.; Barnaby, J.] ARS, Crop Syst & Global Change Lab, USDA, Beltsville, MD 20705 USA. [Bailey, B.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. RP Sicher, R (reprint author), ARS, Crop Syst & Global Change Lab, USDA, Bldg 001,Room 342,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM richard.sicher@ars.usda.gov NR 30 TC 2 Z9 2 U1 3 U2 37 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0300-3604 EI 1573-9058 J9 PHOTOSYNTHETICA JI Photosynthetica PD MAR PY 2015 VL 53 IS 1 BP 3 EP 10 DI 10.1007/s11099-015-0074-9 PG 8 WC Plant Sciences SC Plant Sciences GA CA6KB UT WOS:000349020700002 ER PT J AU Holmes, TRH Crow, WT Hain, C Anderson, MC Kustas, WP AF Holmes, T. R. H. Crow, W. T. Hain, C. Anderson, M. C. Kustas, W. P. TI Diurnal temperature cycle as observed by thermal infrared and microwave radiometers SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Land surface temperature; Diurnal temperature cycle; Thermal infrared; Passive microwave ID LAND-SURFACE TEMPERATURE; SOIL-MOISTURE; BRIGHTNESS TEMPERATURE; SATELLITE DATA; IN-SITU; VALIDATION; MODEL; METHODOLOGY; RETRIEVAL AB Land surface temperature (LST) is a key input to physically-based retrieval algorithms of hydrological states and fluxes, and global measurements of LST are provided by many satellite platforms. Passive microwave (MW) observations offer an alternative to thermal infrared (TIR) LST retrieval approaches. Although MW has a lower spatial resolution, its temporal record is more complete as it is more tolerant to clouds. Moreover, merging TIR and MW LST with independent random errors should result in enhanced LST products. Despite these benefits, MW-based LST retrievals are not widely adopted for land applications except as an input to soil moisture retrieval algorithms. This research aims to facilitate expanded use of MW-based LST by formulating a model to explain the systematic differences in comparison to TIR-LST, and quantifying random errors in each datastream. To that end, we compile a 6-year dataset over the African continent, combining observations from seven intercalibrated low orbiting satellites equipped with suitable microwave radiometers. We compare the diurnal timing and amplitude of this dataset to TIR-LST time-series produced by LSA-SAF from the Meteosat Second Generation geostationary satellite. A third independent data source, the skin temperature as modeled by the Modern Era Reanalysis for Research and Applications (MERRA) as produced at NASA's Global Modeling and Assimilation Office, is included in a triple collocation analysis to calculate the random error in the amplitude anomaly. Results suggest that the anomaly in diurnal LST amplitude over much of the African domain can be estimated from TIR and MW with similar levels of random error for clear sky days, except over dry sandy desert areas where the MW sensing depth extends too far below the surface. The temporal sampling advantage of MW, due to higher cloud tolerance in comparison with TIR retrievals, appears to be substantial but will need to be verified by follow-on studies. Overall the results of this study present a significant step forward in reconciling the systematic differences in LST, preparing the way for a global merger of MW and TIR LST time-series. (C) 2014 Elsevier Inc. All rights reserved. C1 [Holmes, T. R. H.; Crow, W. T.; Anderson, M. C.; Kustas, W. P.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Holmes, T. R. H.] Sci Syst & Applicat, Greenbelt, MD USA. [Hain, C.] Univ Maryland, Earth Syst Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Holmes, TRH (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, BARC West B007,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM thomas.holmes@ARS.USDA.GOV RI Anderson, Martha/C-1720-2015; OI Anderson, Martha/0000-0003-0748-5525; Holmes, Thomas/0000-0002-4651-0079 FU NASA [NNH09ZDA001N-TERRAQUA] FX This work was funded by NASA through the research grant "The Science of Terra and Aqua" (NNH09ZDA001N-TERRAQUA). The authors would like to thank LSA SAF for the dissemination of EUMETSAT products and the Global Modeling and Assimilation Office (GMAO) and GES DISC (both at NASA) for the dissemination of MERRA. NR 38 TC 8 Z9 8 U1 1 U2 32 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 1 PY 2015 VL 158 BP 110 EP 125 DI 10.1016/j.rse.2014.10.031 PG 16 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CA4MR UT WOS:000348879100009 ER PT J AU Burakowski, EA Ollinger, SV Lepine, L Schaaf, CB Wang, Z Dibb, JE Hollinger, DY Kim, JH Erb, A Martin, M AF Burakowski, Elizabeth A. Ollinger, Scott V. Lepine, Lucie Schaaf, Crystal B. Wang, Zhuosen Dibb, Jack E. Hollinger, David Y. Kim, JiHyun Erb, Angel Martin, Mary TI Spatial scaling of reflectance and surface albedo over a mixed-use, temperate forest landscape during snow-covered periods SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE MODIS; Snow; Albedo; Reflectance; Temperate forest; Hyperspectral imagery; Spatial representativeness ID IN-SITU MEASUREMENTS; ACCURACY ASSESSMENT; ATMOSPHERIC CORRECTION; PRODUCT MCD43A; CLIMATE-CHANGE; MODIS DATA; RETRIEVALS; VALIDATION; ALGORITHM; GREENLAND AB Albedo products from the MODerate resolution Imaging Spectroradiometer (MODIS) have been validated extensively over spatially homogeneous sites with snow-cover. This study evaluated the spatial scaling of albedo and related reflectance-based quantities over areas of high spatial heterogeneity in temperate mixed forest, deciduous forest, urban and built-up, and cropland/natural mosaic lands under snow covered conditions. Reflectance-based quantities evaluated included spectral radiance, surface directional reflectance, and spectral and broadband albedo derived from ground- and tower-based measurements and high-resolution (5 m) hyperspectral imagery (HSI) to coarse resolution (similar to 500 m) MODIS satellite data. Our approach first compared ground- and tower-based spectral and broadband reflectance quantities to HSI data, then to evaluate HSI with MODIS reflectance-based products. Over snow-covered pasture, HSI directional reflectance was biased lower than ground-based measurements collected using a spectroradiometer, and greatly underestimated at wavelengths less than 450 nm. Tower-based shortwave broadband albedo (280-2800 nm) and HSI albedo agreed within +/- 0.04. HSI directional spectral reflectance agreed well with tower-based measurements of spectral albedo collected using a spectroradiometer and remote cosine receptor above a mixed forest canopy with underlying snow cover. Spectral albedo collected over a dormant deciduous broadleaf canopy increased from 0.10 for snow-free conditions to 0.14-0.18 when snow-cover was present under the canopy. Canopy shortwave broadband albedo was not very sensitive to underlying snowpack depth, indicative of strong vegetation masking. Next, HSI data were spatially aggregated and averaged to 500 m MODIS grids and compared to two MODIS albedo products: (1) MODIS/Terra and Aqua Combined Bidirectional Reflectance Distribution Function (BRDF) Albedo V005 (MCD43A, magnitude inversion), and (2) MODIS/Ferra Snow Cover Daily 13 Global 500 m Grid (MOD10A1). An assessment of surface heterogeneity demonstrated that MODIS products generally perform well for snow-covered landscapes with high spatial heterogeneity, with biases between +/- 0.04 and RMSEs less than 0.085 for mixed and deciduous broadleaf forested and urban & built-up land cover classes, and less than 0.11 for cropland/natural mosaic. Biases were generally lower for MOD10A1 compared to MCD43A3. Mean shortwave broadband albedo from MODIS and HSI over deciduous broadleaf and mixed forest with underlying snow cover ranged from 0.14 to 028. Albedo over MODIS grids characterized as a mosaic of cropland, forests, grasslands, and shrublands (e.g., cropland/natural mosaic) was substantially higher than forested grids under snow-covered conditions, ranging from 039 to 0.43. (C) 2014 Elsevier Inc All rights reserved. C1 [Burakowski, Elizabeth A.; Ollinger, Scott V.; Lepine, Lucie; Dibb, Jack E.; Martin, Mary] Univ New Hampshire, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. Univ Massachusetts, Coll Sci & Math, Sch Environm, Boston, MA 02125 USA. [Hollinger, David Y.] USDA, Forest Serv Northern Res Stn, Durham, NH 03824 USA. [Kim, JiHyun] Boston Univ, Dept Geog & Environm, Ctr Remote Sensing, Boston, MA 02115 USA. RP Burakowski, EA (reprint author), Natl Ctr Atmospher Res, 1850 Table Mesa Dr, Boulder, CO 80305 USA. EM burakows@ncar.edu; lucie.lepine@unh.edu; crystal.schaaf@umb.edu; zhuosen.wang@umb.edu; jack.dibb@unh.edu; davidh@hypatia.unh.edu; jhkim26@bu.edu; Angela.Erb001@umb.edu; mary.martin@unh.edu RI Burakowski, Elizabeth/E-5502-2015; Hollinger, David/G-7185-2012; Ollinger, Scott/N-3380-2014; OI Burakowski, Elizabeth/0000-0001-5429-9886; Ollinger, Scott/0000-0001-6226-1431; Lepine, Lucie/0000-0003-1028-2534 FU New Hampshire Ecosystems and Society National Science Foundation Experimental Program to Stimulate Competitive Research [EPS-1101245]; National Aeronautic and Space Administration Terrestrial Ecology Program [NNX11AB88G]; NSF [0832652]; Alexander Goetz Instrument Fellowship Program FX This research was supported by the New Hampshire Ecosystems and Society National Science Foundation Experimental Program to Stimulate Competitive Research (Award EPS-1101245), the National Aeronautic and Space Administration Terrestrial Ecology Program (Grant #NNX11AB88G), and the NSF (DEB#0832652) Hubbard Brook Long Term Ecological Research (LTER) project. The use of the ASD FieldSpec4 in March 2012 was provided by the Alexander Goetz Instrument Fellowship Program (2011/2012). The authors would also like to thank the Richardson Lab at Harvard University for loan of an ASD spectroradiometer on 2013-03-09. The authors thank the USDA-Forest Service for loan of the Kipp and Zonen CMA6 at Thompson Farm Research Station, and Bob Evans of the USDA Forest Service for technical assistance. NR 48 TC 4 Z9 4 U1 6 U2 36 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 1 PY 2015 VL 158 BP 465 EP 477 DI 10.1016/j.rse.2014.11.023 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CA4MR UT WOS:000348879100034 ER PT J AU Yen, H Jeong, JH Feng, QY Deb, D AF Yen, Haw Jeong, Jaehak Feng, QingYu Deb, Debjani TI Assessment of Input Uncertainty in SWAT Using Latent Variables SO WATER RESOURCES MANAGEMENT LA English DT Article DE Input uncertainty; Model calibration; SWAT; Uncertainty analysis ID WATER ASSESSMENT-TOOL; MODELS; SOIL AB Applications of the Soil and Water Assessment Tool (SWAT) require a large amount of input data to perform model simulations. Consequently, uncertainty in input data tends to influence the accuracy of SWAT hydrologic and water quality outputs. It has been shown that input uncertainty can be quantified explicitly during model calibration with latent variables. In this study, latent variables were explored to examine their sensitivity to SWAT outputs and further the potential impact of input uncertainty to model predictions. Results show that the increases in the range of latent variables pose a significant influence to streamflow and ammonia predictions while the impact was less significant in sediment responses. The performance of SWAT in predicting streamflow and ammonia declined with wider ranges of latent variables. In addition, the increase in the range of latent variables did not present noticeable effect on the corresponding predictive uncertainty in sediment predictions. In this study, the calibration results did not improve significantly with the applications of wider ranges of latent variables which are different from the findings in previous research work. The use of latent variables to incorporate input uncertainty may not be the proper alternative choice in terms of generating better results and should be carefully evaluated in the implementations of complex watershed simulation models. C1 [Yen, Haw; Jeong, Jaehak] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Yen, Haw] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Feng, QingYu] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Deb, Debjani] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. RP Yen, H (reprint author), USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. EM hyen@brc.tamus.edu FU United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components FX This study was supported by the funding from the United States Department of Agriculture - Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP) - Wildlife and Cropland components. Awesome comments provided by reviewers and editorial board greatly improved the quality of the manuscript. Thanks a lot! In addition, please remember that USDA is an equal opportunity employer and provider! NR 26 TC 9 Z9 9 U1 2 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-4741 EI 1573-1650 J9 WATER RESOUR MANAG JI Water Resour. Manag. PD MAR PY 2015 VL 29 IS 4 BP 1137 EP 1153 DI 10.1007/s11269-014-0865-y PG 17 WC Engineering, Civil; Water Resources SC Engineering; Water Resources GA CB1BK UT WOS:000349361200011 ER PT J AU Alvarado-Esquivel, C Vazquez-Morales, RF Colado-Romero, EE Guzman-Sanchez, R Liesenfeld, O Dubey, JP AF Alvarado-Esquivel, Cosme Fabiola Vazquez-Morales, Renata Eusebio Colado-Romero, Edgar Guzman-Sanchez, Ramiro Liesenfeld, Oliver Dubey, Jitender P. TI PREVALENCE OF INFECTION WITH TOXOPLASMA GONDII IN LANDRACE AND MIXED BREED PIGS SLAUGHTERED IN BAJA CALIFORNIA SUR STATE, MEXICO SO EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY LA English DT Article DE pigs; Toxoplasma gondii; seroprevalence; epidemiology; Mexico ID DOMESTIC PIGS; SEROEPIDEMIOLOGY; SEROPREVALENCE; PATHOGENICITY; DURANGO AB We performed a cross-sectional study to determine the seroprevalence of Toxoplasma gondii infection in 308 domestic pigs slaughtered in La Paz, Baja California Sur State, Mexico using the modified agglutination test (MAT, cut off 1:25). Forty (13%) of the 308 pigs were seropositive with MAT titers of 1:25 in 16, 1:50 in 5, 1:100 in 4, 1:200 in 5, 1:400 in 3, 1:800 in 3, 1:1600 in 2, and 1:3200 in 2. Multivariate analysis of pigs' characteristics showed that seropositivity to T. gondii was negatively associated with mixed breed (OR = 0.02; 95% CI:0.003-0.26; P = 0.001). Other variables including sex, type of raising, and municipality did not show an association with T. gondii seropositivity by multivariate analysis. The frequency of high antibody titers (>= 1:400) was significantly higher (P < 0.001) in Landrace pigs than mixed breed pigs. The seroprevalence of T. gondii infection in pigs for slaughter in Baja California Sur State is low compared with seroprevalences reported in pigs in other Mexican states. Landrace pigs demonstrated higher seroprevalence rates and antibody levels than mixed breed pigs. This is the first report of T. gondii infection in pigs raised in a desert climate. C1 [Alvarado-Esquivel, Cosme] Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Ave Univ S-N, Durango 34000, Dgo, Mexico. [Fabiola Vazquez-Morales, Renata; Eusebio Colado-Romero, Edgar; Guzman-Sanchez, Ramiro] Comite Estatal Fomento & Protecc Pecuaria, La Paz, Bolivia. [Liesenfeld, Oliver] Charite, Inst Microbiol & Hyg, Campus Benjamin Franklin, Hindenburgdamm 27, D-12203 Berlin, Germany. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. RP Alvarado-Esquivel, C (reprint author), Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Ave Univ S-N, Durango 34000, Dgo, Mexico. EM alvaradocosme@yahoo.com NR 18 TC 1 Z9 1 U1 0 U2 0 PU AKADEMIAI KIADO RT PI BUDAPEST PA PRIELLE K U 19, PO BOX 245,, H-1117 BUDAPEST, HUNGARY SN 2062-8633 J9 EUR J MICROBIOL IMMU JI Eur. J. Microbiol. Immunol. PD MAR PY 2015 VL 5 IS 1 BP 112 EP 115 DI 10.1556/EuJMI-D-15-00004 PG 4 WC Microbiology SC Microbiology GA DG5QJ UT WOS:000372132600010 PM 25883799 ER PT J AU Cintron, MS Hinchliffe, DJ AF Cintron, Michael Santiago Hinchliffe, Doug J. TI FT-IR Examination of the Development of Secondary Cell Wall in Cotton Fibers (dagger) SO FIBERS LA English DT Article DE cell wall; cotton; FT-IR; moisture; maturity ID TRANSFORM INFRARED-SPECTROSCOPY; CELLULOSE; ACCESSIBILITY; GERMPLASM; STRENGTH; MATURITY; GROWTH AB The secondary cell wall development of cotton fibers harvested at 18, 20, 24, 28, 32, 36 and 40 days after flowering was examined using attenuated total reflection Fourier transform-infrared (ATR FT-IR) spectroscopy. Spectra of deuterated cotton fibers did not demonstrate significant changes in their O-H stretching band shapes or positions during development. Only a progressive increase in O-H band intensity was observed. Results indicate that the highly crystalline cellulose component produced during secondary cell wall formation maintains the hydrogen bonding network observed for the primary cell wall. Other general changes were observed for the regular ATR spectra. A progressive intensity increase for bands assigned to cellulose I-beta was observed during fiber development, including a marked intensity increase for vibrations at 1002 and 985 cm(-1). In contrast, C-O vibrational bands from dominant conformations observed at 1104, 1052, 1028 cm(-1) undergo a modest intensity increase during secondary cell wall development. C1 [Cintron, Michael Santiago] ARS, Cotton Struct & Qual Res Unit, SRRC, USDA, New Orleans, LA 70124 USA. [Hinchliffe, Doug J.] ARS, Cotton Chem & Utilizat Res Unit, SRRC, USDA, New Orleans, LA 70124 USA. RP Cintron, MS (reprint author), ARS, Cotton Struct & Qual Res Unit, SRRC, USDA, New Orleans, LA 70124 USA. EM michael.santigo@ars.usda.gov; doug.hinchliffe@ars.usda.gov NR 23 TC 4 Z9 4 U1 0 U2 1 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2079-6439 J9 FIBERS JI Fibers PD MAR PY 2015 VL 3 IS 1 BP 30 EP 40 DI 10.3390/fib3010030 PG 11 WC Materials Science, Textiles SC Materials Science GA DG0FR UT WOS:000371741000002 ER PT J AU King, KW Williams, MR Macrae, ML Fausey, NR Frankenberger, J Smith, DR Kleinman, PJA Brown, LC AF King, Kevin W. Williams, Mark R. Macrae, Merrin L. Fausey, Norman R. Frankenberger, Jane Smith, Douglas R. Kleinman, Peter J. A. Brown, Larry C. TI Phosphorus Transport in Agricultural Subsurface Drainage: A Review SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID QUANTIFYING PREFERENTIAL FLOW; WATER-QUALITY PROTECTION; ATLANTIC COASTAL-PLAIN; INDUSTRIAL BY-PRODUCTS; GULF-OF-MEXICO; TILE DRAINAGE; CLAY SOIL; SURFACE RUNOFF; CONSTRUCTED WETLANDS; MANURE APPLICATION AB Phosphorus (P) loss from agricultural fields and watersheds has been an important water quality issue for decades because of the critical role P plays in eutrophication. Historically, most research has focused on P losses by surface runoff and erosion because subsurface P losses were often deemed to be negligible. Perceptions of subsurface P transport, however, have evolved, and considerable work has been conducted to better understand the magnitude and importance of subsurface P transport and to identify practices and treatments that decrease subsurface P loads to surface waters. The objectives of this paper were (i) to critically review research on P transport in subsurface drainage, (ii) to determine factors that control P losses, and (iii) to identify gaps in the current scientific understanding of the role of subsurface drainage in P transport. Factors that affect subsurface P transport are discussed within the framework of intensively drained agricultural settings. These factors include soil characteristics (e. g., preferential flow, P sorption capacity, and redox conditions), drainage design (e. g., tile spacing, tile depth, and the installation of surface inlets), prevailing conditions and management (e. g., soil-test P levels, tillage, cropping system, and the source, rate, placement, and timing of P application), and hydrologic and climatic variables (e. g., baseflow, event flow, and seasonal differences). Structural, treatment, and management approaches to mitigate subsurface P transport-such as practices that disconnect flow pathways between surface soils and tile drains, drainage water management, in-stream or end-of-tile treatments, and ditch design and management-are also discussed. The review concludes by identifying gaps in the current understanding of P transport in subsurface drains and suggesting areas where future research is needed. C1 [King, Kevin W.; Williams, Mark R.; Fausey, Norman R.] ARS, USDA, Soil Drainage Res Unit, Columbus, OH 43210 USA. [Macrae, Merrin L.] Univ Waterloo, Dept Geog & Environm Management, Waterloo, ON N2L 3G1, Canada. [Frankenberger, Jane] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Smith, Douglas R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Kleinman, Peter J. A.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Brown, Larry C.] Ohio State Univ, Dept Food Agr & Biol Engn, Columbus, OH 43210 USA. RP King, KW (reprint author), ARS, USDA, Soil Drainage Res Unit, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM kevin.king@ars.usda.gov NR 239 TC 35 Z9 35 U1 2 U2 2 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 467 EP 485 DI 10.2134/jeq2014.04.0163 PG 19 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000017 PM 26023966 ER PT J AU King, KW Williams, MR Fausey, NR AF King, Kevin W. Williams, Mark R. Fausey, Norman R. TI Contributions of Systematic Tile Drainage to Watershed-Scale Phosphorus Transport SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID MANAGING AGRICULTURAL PHOSPHORUS; QUALITY PROTECTION; SUBSURFACE; SURFACE; PATHWAYS; DITCHES; CATCHMENT; LOSSES; EXPORT; CONSERVATION AB Phosphorus (P) transport from agricultural fields continues to be a focal point for addressing harmful algal blooms and nuisance algae in freshwater systems throughout the world. In humid, poorly drained regions, attention has turned to P delivery through subsurface tile drainage. However, research on the contributions of tile drainage to watershed-scale P losses is limited. The objective of this study was to evaluate long-term P movement through tile drainage and its manifestation at the watershed outlet. Discharge data and associated P concentrations were collected for 8 yr (2005-2012) from six tile drains and from the watershed outlet of a headwater watershed within the Upper Big Walnut Creek watershed in central Ohio. Results showed that tile drainage accounted for 47% of the discharge, 48% of the dissolved P, and 40% of the total P exported from the watershed. Average annual total P loss from the watershed was 0.98 kg ha(-1), and annual total P loss from the six tile drains was 0.48 kg ha(-1). Phosphorus loads in tile and watershed discharge tended to be greater in the winter, spring, and fall, whereas P concentrations were greatest in the summer. Over the 8-yr study, P transported in tile drains represented <2% of typical application rates in this watershed, but >90% of all measured concentrations exceeded recommended levels (0.03 mg L-1) for minimizing harmful algal blooms and nuisance algae. Thus, the results of this study show that in systematically tile-drained headwater watersheds, the amount of P delivered to surface waters via tile drains cannot be dismissed. Given the amount of P loss relative to typical application rates, development and implementation of best management practices (BMPs) must jointly consider economic and environmental benefits. Specifically, implementation of BMPs should focus on late fall, winter, and early spring seasons when most P loading occurs. C1 [King, Kevin W.; Williams, Mark R.; Fausey, Norman R.] ARS, USDA, Columbus, OH 43210 USA. RP King, KW (reprint author), ARS, USDA, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM kevin.king@ars.usda.gov NR 48 TC 23 Z9 23 U1 0 U2 0 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAR-APR PY 2015 VL 44 IS 2 BP 486 EP 494 DI 10.2134/jeq2014.04.0149 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CD6MU UT WOS:000351204000018 PM 26023967 ER PT J AU Register, KB Ivanov, YV Harvill, ET Davison, N Foster, G AF Register, Karen B. Ivanov, Yury V. Harvill, Eric T. Davison, Nick Foster, Geoffrey TI Novel, host-restricted genotypes of Bordetella bronchiseptica associated with phocine respiratory tract isolates SO MICROBIOLOGY-SGM LA English DT Article ID FIELD GEL-ELECTROPHORESIS; ADENYLATE-CYCLASE TOXIN; DISTEMPER EPIDEMIC; ESCHERICHIA-COLI; PERTACTIN; PARAPERTUSSIS; IDENTIFICATION; PERTUSSIS; EVOLUTION; SEQUENCE AB During a succession of phocine morbillivirus outbreaks spanning the past 25 years, Bordetella bronchiseptica was identified as a frequent secondary invader and cause of death. The goal of this study was to evaluate genetic diversity and the molecular basis for host specificity among seal isolates from these outbreaks. MLST and Pvull ribotyping of 54 isolates from Scottish, English or Danish coasts of the Atlantic or North Sea revealed a single, host-restricted genotype. A single, novel genotype, unique from that of the Atlantic and North Sea isolates, was found in isolates from an outbreak in the Caspian Sea. Phylogenetic analysis based either on MLST sequence, ribotype patterns or genome-wide SNPs consistently placed both seal-specific genotypes within the same major Glade but indicates a distinct evolutionary history for each. An additional isolate from the intestinal tract of a seal on the south-west coast of England has a genotype otherwise found in rabbit, guinea pig and pig isolates. To investigate the molecular basis for host specificity, DNA and predicted protein sequences of virulence genes that mediate host interactions were used in comparisons between a North Sea isolate, a Caspian Sea isolate and each of their closest relatives as inferred from genome-wide SNP analysis. Despite their phylogenetic divergence, fewer nucleotide and amino acid substitutions were found in comparisons of the two seal isolates than in comparisons with closely related strains. These data indicate isolates of B. bronchiseptica associated with respiratory disease in seals comprise unique, host-adapted and highly clonal populations. C1 [Register, Karen B.] ARS, USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. [Ivanov, Yury V.; Harvill, Eric T.] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Davison, Nick; Foster, Geoffrey] Scottish Marine Anim Stranding Scheme SAC Consult, Inverness IV2 4JZ, Scotland. RP Register, KB (reprint author), ARS, USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM karen.register@ars.usda.gov FU National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [M85/00/2, M435/02/3, HHSN272200900007C] FX Sequencing of M85/00/2 and M435/02/3 was funded, in part, by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services (contract HHSN272200900007C). We gratefully acknowledge the excellent technical assistance of William Boatwright and Gwen Nordholm and the contributions of James Barnett, who conducted post-mortem evaluation and provided the clinical history of the seal that was the source of isolate 22/M63/4/09. Harry Ross, Tony Patterson, Jason Barley and Thijs Kuiken are acknowledged for provision of material from seal carcasses from which isolates were recovered. We thank Lea Ann Hobbs and David Alt at the NADC Genomics Unit for DNA sequence data derived from PCR amplicons. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 49 TC 4 Z9 4 U1 1 U2 4 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1350-0872 J9 MICROBIOL-SGM JI Microbiology-(UK) PD MAR PY 2015 VL 161 BP 580 EP 592 DI 10.1099/mic.0.000035 PN 3 PG 13 WC Microbiology SC Microbiology GA DM0PJ UT WOS:000376048000011 PM 25627438 ER PT J AU Damm, DL Armstrong, JB Arjo, WM Piaggio, AJ AF Damm, Dalinda L. Armstrong, James B. Arjo, Wendy M. Piaggio, Antoinette J. TI Assessment of Population Structure of Coyotes in East-Central Alabama using Microsatellite DNA SO SOUTHEASTERN NATURALIST LA English DT Article ID SOUTHERN CALIFORNIA; RURAL GRADIENT; CANIS-LATRANS; HOME-RANGE; MANAGEMENT; CONSERVATION; MARKERS; DIFFERENTIATION; IDENTIFICATION; LANDSCAPE AB Canis latrans (Coyotes) are a management concern in the southeastern US because of their potential impacts on agriculture, other wildlife species, and human health and safety. This region is part of a recent range expansion by Coyotes, and information about their population structure in the southeastern US is lacking. In this study, we used microsatellite DNA to assess genetic diversity and population structure among Coyotes in east-central Alabama. We detected high genetic diversity (H-E = 0.78) and no population structure across the total sampling area. Additionally, we investigated population structure between urban and rural groups. We detected low but significant population structure between these groups, which may be biologically meaningful. We discuss the implications of this result in the context of potential management strategies. Overall, our study sought to provide information about the molecular ecology of Coyotes within a region of recent range expansion. C1 [Damm, Dalinda L.; Armstrong, James B.] Auburn Univ, Sch Forestry & Wildlife Sci, 602 Duncan Dr, Auburn, AL 36849 USA. [Arjo, Wendy M.] AGEISS Inc, Lacey, WA 98516 USA. [Piaggio, Antoinette J.] APHIS, USDA, WS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Damm, DL (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, 602 Duncan Dr, Auburn, AL 36849 USA. EM Dalinda_Damm@yahoo.com FU Center for Forest Sustainability and Auburn University; USDA-National Wildlife Research Center's Genetics Lab; Kenny Brock Lab at the Auburn University School of Veterinary Medicine FX We thank The Center for Forest Sustainability and Auburn University for funding this study. We thank the USDA-National Wildlife Research Center's Genetics Lab and the Kenny Brock Lab at the Auburn University School of Veterinary Medicine for support with data collection and analyses. Also, thanks to all of the individuals and agencies that collected carcasses and tissue samples for this project, including Alabama USDA-Wildlife Services. Thank you to Dr. Todd Steury for helping tremendously with statistical analyses, Amy Silvano for assistance with ArcGIS, and Karen Tenaglia-Hoksbergen, Dr. Scott Santos,and Philip Damm for frequent help and guidance. Lastly, we appreciate the comments provided by reviewers of this manuscript during consideration of publication. NR 82 TC 0 Z9 0 U1 4 U2 5 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 EI 1938-5412 J9 SOUTHEAST NAT JI Southeast. Nat. PD MAR PY 2015 VL 14 IS 1 BP 106 EP 122 DI 10.1656/058.014.0118 PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE8NJ UT WOS:000370892400010 ER PT J AU Irvine, KM Miller, SW Al-Chokhachy, RK Archer, EK Roper, BB Kershner, JL AF Irvine, Kathryn M. Miller, Scott W. Al-Chokhachy, Robert K. Archer, Eric K. Roper, Brett B. Kershner, Jeffrey L. TI Empirical evaluation of the conceptual model underpinning a regional aquatic long-term monitoring program using causal modelling SO ECOLOGICAL INDICATORS LA English DT Article DE Adaptive monitoring; Grazing; Heterogeneous landscapes; Long-term monitoring; Mediator; Moderator; Macroinvertebrates; Path analysis; Roads; Sedimentation ID WESTERN UNITED-STATES; LAND-USE; STREAM HABITAT; PACIFIC-NORTHWEST; BIOLOGICAL INTEGRITY; NATURAL-RESOURCES; HEADWATER STREAMS; PREDICTIVE MODELS; SEDIMENT CRITERIA; MOUNTAIN STREAMS AB Conceptual models are an integral facet of long-term monitoring programs. Proposed linkages between drivers, stressors, and ecological indicators are identified within the conceptual model of most mandated programs. We empirically evaluate a conceptual model developed for a regional aquatic and riparian monitoring program using causal models (i.e., Bayesian path analysis). We assess whether data gathered for regional status and trend estimation can also provide insights on why a stream may deviate from reference conditions. We target the hypothesized causal pathways for how anthropogenic drivers of road density, percent grazing, and percent forest within a catchment affect instream biological condition. We found instream temperature and fine sediments in arid sites and only fine sediments in mesic sites accounted for a significant portion of the maximum possible variation explainable in biological condition among managed sites. However, the biological significance of the direct effects of anthropogenic drivers on instream temperature and fine sediments were minimal or not detected. Consequently, there was weak to no biological support for causal pathways related to anthropogenic drivers' impact on biological condition. With weak biological and statistical effect sizes, ignoring environmental contextual variables and covariates that explain natural heterogeneity would have resulted in no evidence of human impacts on biological integrity in some instances. For programs targeting the effects of anthropogenic activities, it is imperative to identify both land use practices and mechanisms that have led to degraded conditions (i.e., moving beyond simple status and trend estimation). Our empirical evaluation of the conceptual model underpinning the long-term monitoring program provided an opportunity for learning and, consequently, we discuss survey design elements that require modification to achieve question driven monitoring, a necessary step in the practice of adaptive monitoring. We suspect our situation is not unique and many programs may suffer from the same inferential disconnect. Commonly, the survey design is optimized for robust estimates of regional status and trend detection and not necessarily to provide statistical inferences on the causal mechanisms outlined in the conceptual model, even though these relationships are typically used to justify and promote the long-term monitoring of a chosen ecological indicator. Our application demonstrates a process for empirical evaluation of conceptual models and exemplifies the need for such interim assessments in order for programs to evolve and persist. Published by Elsevier Ltd. C1 [Irvine, Kathryn M.; Al-Chokhachy, Robert K.; Kershner, Jeffrey L.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Miller, Scott W.] Utah State Univ, Dept Watershed Sci, BLM Natl Aquat Monitoring Ctr, Logan, UT 84322 USA. [Archer, Eric K.; Roper, Brett B.] USDA, Forest Serv, Forest Sci Lab, Logan, UT 84321 USA. RP Irvine, KM (reprint author), US Geol Survey, Northern Rocky Mt Sci Ctr, 2327 Univ Way,Suite 2, Bozeman, MT 59715 USA. EM kirvine@usgs.gov RI Miller, Scott/H-6548-2011 NR 104 TC 3 Z9 3 U1 1 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD MAR PY 2015 VL 50 BP 8 EP 23 DI 10.1016/j.ecolind.2014.10.011 PG 16 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AZ5MO UT WOS:000348265200002 ER PT J AU Lentz, RD AF Lentz, Rodrick D. TI Polyacrylamide and biopolymer effects on flocculation, aggregate stability, and water seepage in a silt loam SO GEODERMA LA English DT Article DE Polymer; Soil aggregate; Infiltration; Seepage ID ANIONIC POLYACRYLAMIDE; MOLECULAR-WEIGHT; STEEP SLOPES; SOIL; INFILTRATION; POLYMERS; EROSION; POLYSACCHARIDES; MONTMORILLONITE; IRRIGATION AB Researchers seek a more renewable and natural alternative for water soluble anionic polyacrylamide (PAM), a highly-effective, petroleum-derived polymer used in agriculture to control erosion and reduce water seepage from unlined irrigation structures. This study evaluated four anionic polymers including low- (0.5 Mg mol(-1)) or high molecular-weight (MW) (10-20 Mg mol(-1)), bacteria-produced polysaccharides (biopolymers) and low- (0.2 Mg mol(-1)) or high-MW (12-15 Mg mol(-1)) PAMs. We evaluated their influence on (1) the rate and efficacy of colloid flocculation in 1- and 10-mg L-1, high-MW, PAM or biopolymer solutions; (2) percent water stable aggregates in silt loam soil treated with water or 1000-mg L-1 solutions of the four polymers; and (3) effective saturated hydraulic conductivity (seepage loss) when water or 500-mg L-1, high-MW biopolymer or PAM solutions were applied to silt loam. The biopolymer most effectively flocculated the colloids at a concentration of 1 mg L-1, but was still 30% less effective than PAM at 1 mg L-1 and 50% less effective than PAM at 10 mg L-1. Overall, both polymer types increased the percent of stable soil aggregates in the silt loam, 1-to-2-mm-diam. size fraction, though PAM produced 135-times more stable aggregates than the biopolymer, 88.7% vs. 65.5%. These results suggested that the biopolymer's bulkier molecular conformation limited the extension and flexibility of the molecule in solution, compared to PAM. After 140 h, the biopolymer reduced seepage loss rates by 21%, while PAM increased loss rates 1.6-fold, compared to controls. These data suggest that the biopolymer would be less effective than PAM for reducing water erosion owing to its lesser flocculation and aggregate-stabilizing potential. However, the biopolymer could be a more desirable alternative to PAM for controlling seepage from unlined irrigation canals and reservoirs; it (i) can be used effectively at lower concentrations, (ii) is considered more environmentally friendly, and (iii) is produced from a renewable resource. Published by Elsevier B.V. C1 ARS, USDA, Northwest Irrigat & Soils Res Lab, Kimberly, ID 83341 USA. RP Lentz, RD (reprint author), ARS, USDA, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM rick.lentz@ars.usda.gov NR 41 TC 5 Z9 5 U1 7 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD MAR PY 2015 VL 241 BP 289 EP 294 DI 10.1016/j.geoderma.2014.11.019 PG 6 WC Soil Science SC Agriculture GA AZ2SX UT WOS:000348083700028 ER PT J AU Zhu, Q Schmidt, JP Bryant, RB AF Zhu, Qing Schmidt, John P. Bryant, Ray B. TI Maize (Zea mays L.) yield response to nitrogen as influenced by spatio-temporal variations of soil-water-topography dynamics SO SOIL & TILLAGE RESEARCH LA English DT Article DE Hydropedology; Non-point contamination; Precision agriculture; Soil moisture ID TEMPORAL STABILITY; AGRICULTURAL LANDSCAPE; SPATIAL VARIABILITY; MOISTURE; CORN; FIELD; FERTILIZER; MINERALIZATION; AVAILABILITY; MANAGEMENT AB Applying N fertilizer at rates that satisfy both economic and environmental objectives are critical for sustainable agriculture. The hypothesis of this study was that the spatial variability in maize (Zea mays L) yield and its response to N rate were influenced by soil-water-topography dynamics. In 2008 and 2009, a study was conducted along an agricultural hillslope in the Northern Appalachian Ridge and Valley Physiographic Province in the USA with Cambisols according to FAO soil classification. Minimum, maximum, and delta yields and optimum N rate at different slope positions were determined using quadratic-plateau maize yield - N rate models. Results confirmed our hypothesis. The spatial variability of maize yield and its response to N rate was influenced by silt content, soil depth, profile curvature, slope, soil wetness and degree of soil water content temporal variation. In both dry year (2008) and wet year (2009), optimum N rates positively correlated (P < 0.05) with the temporal variation of soil water content, which is an indicator of subsurface flow paths. In 2008, maize yield was little varied along this hillslope (11.7-12.0 Mg ha(-1)), while greater yield response to N rate (represented as delta yield, 5.6 Mg ha(-1)) was observed in upper convex and steep slope areas with low minimum yield (6.1 Mg ha(-1)). However, in 2009, greater maximum maize yield (13.5 Mg ha(-1)) and yield response to N rate (8.7 Mg ha(-1)) were observed in lower concave slope areas with deeper soil depth and thus greater water storage. Results from this study suggested that site-specific N applications could be improved by considering within field variability of soil, topography and hydrology. (C) 2014 Elsevier B.V. All rights reserved. C1 [Zhu, Qing] Chinese Acad Sci, Nanjing Inst Geog & Limnol, Key Lab Watershed Geog Sci, Nanjing 210008, Jiangsu, Peoples R China. [Zhu, Qing; Bryant, Ray B.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Schmidt, John P.] Pioneer HiBred Int Inc, Champaign Res Ctr, Ivesdale, IL 61851 USA. RP Zhu, Q (reprint author), Chinese Acad Sci, Nanjing Inst Geog & Limnol, Key Lab Watershed Geog Sci, 73 East Beijing Rd, Nanjing 210008, Jiangsu, Peoples R China. EM qzhu@niglas.ac.cn FU National Natural Science Foundation of China [41271109]; Jiangsu Natural Science Foundation [BK2012502]; Key "135" Project of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences [NIGLAS2012135005] FX The data collection of this study was supported by the USDA-ARS Pasture Systems and Watershed Management Research Unit. The preparation of this paper was supported by the National Natural Science Foundation of China (41271109), Jiangsu Natural Science Foundation (Grant no. BK2012502) and Key "135" Project of Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences (NIGLAS2012135005). NR 49 TC 7 Z9 9 U1 2 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD MAR PY 2015 VL 146 BP 174 EP 183 DI 10.1016/j.still.2014.10.006 PN B PG 10 WC Soil Science SC Agriculture GA AY3RA UT WOS:000347499100006 ER PT J AU Strauss, SL Stover, JK Kluepfel, DA AF Strauss, S. L. Stover, J. K. Kluepfel, D. A. TI Impact of biological amendments on Agrobacterium tumefaciens survival in soil SO APPLIED SOIL ECOLOGY LA English DT Article DE Vermicompost; Agrobacterium tumefaciens; Microbial communities; Walnuts; Soil fumigation ID MICROBIAL FUNCTIONAL DIVERSITY; METHYL-BROMIDE; FLUORESCENT PSEUDOMONADS; BIOORGANIC FERTILIZER; COMMUNITY COMPOSITION; RHIZOCTONIA-SOLANI; COLEUS-FORSKOHLII; ENZYME-ACTIVITIES; FUSARIUM-WILT; ROOT-ROT AB Paradox, the primary walnut rootstock used in California, is susceptible to Agrobacterium tumefaciens, which causes crown gall. While A. tumefaciens is susceptible to commonly used fumigants such as methyl bromide (MeBr) and Telone-C35 (1,3-dichloropropene and chloropicrin), these fumigants also significantly modify the indigenous soil microbial community, which impacts the ability to inhibit A. tumefaciens populations introduced into these soils. We hypothesized increasing microbial diversity and activity in fumigated soil would provide greater competition for A. tumefaciens, thereby reducing its abundance and limiting disease incidence. Three commercially available soil amendments at multiple rates were tested in native and Telone-C35 fumigated soils in laboratory experiments: vermicompost and two commercial microbial fermentation mixtures marketed as soil amendments. The amendment-soil mixture was infested with a rifampicin-resistant mutant of A. tumefaciens whose abundance was determined by dilution plating and quantitative PCR over the course of four weeks. The two commercial fermentation amendments had no effect on A. tumefaciens population dynamics. However, after a four-week exposure to 10% (w/w) or greater vermicompost, A. tumefaciens populations declined, and in some cases dipped, below detection limits. This suppressive effect appeared to be predominately biotic as heat-treated vermicompost had no impact on A. tumefaciens populations. In addition, culture independent analysis using the Shannon index of diversity found a positive correlation between A. tumefaciens suppression and microbial community diversity. The vermicompost used in this study also provided a consistently similar microbial community in individual batches used over a 9-month period, making it suitable for reliable commercial use. Published by Elsevier B.V. C1 [Strauss, S. L.; Stover, J. K.; Kluepfel, D. A.] Univ Calif Davis, USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Kluepfel, DA (reprint author), Univ Calif Davis, USDA ARS, Crops Pathol & Genet Res Unit, 1 Shields Ave, Davis, CA 95616 USA. EM dakluepfel@ucdavis.edu RI Strauss, Sarah/I-5141-2016 OI Strauss, Sarah/0000-0001-7494-5320 FU CDFA Fruit, Nut and Grapevine Improvement Advisory Board; USDA-ARS CRIS [5306-22000-015-00D] FX We thank Ali E. McClean, Rachel F. Greenhut, and Luis S. Curiel for their technical assistance. We also thank Jack Chambers of Terra Vesco for supplying vermicompost and Mike Stanghellini and Matt Gillis of Tri-Cal for soil fumigation assistance. This work was partly funded by the CDFA Fruit, Nut and Grapevine Improvement Advisory Board and USDA-ARS CRIS 5306-22000-015-00D. NR 68 TC 2 Z9 2 U1 2 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD MAR PY 2015 VL 87 BP 39 EP 48 DI 10.1016/j.apsoil.2014.10.016 PG 10 WC Soil Science SC Agriculture GA AX0LQ UT WOS:000346644300006 ER PT J AU Wang, AQ Ye, XZ Huang, JL Niu, JQ Liu, M Pan, YB Yang, LT Li, YR AF Wang, Ai-Qin Ye, Xing-Zhi Huang, Jing-Li Niu, Jun-Qi Liu, Ming Pan, Yong-Bao Yang, Li-Tao Li, Yang-Rui TI Molecular Cloning and Functional Analysis of an Ethylene Receptor Gene from Sugarcane (Saccharum spp.) by Hormone and Environmental Stresses SO SUGAR TECH LA English DT Article DE Ethylene receptors; Cloning; Sugarcane; Gene expression; Real-time qPCR ID ARABIDOPSIS AB Ethylene receptor (ethylene response sensor, ERS) is the primary component involving in the ethylene biosynthesis and ethylene signal transduction pathway. In the present study, a GZ-ERS gene encoding ERS was cloned from a sugarcane cv. YL17 (Saccharum spp.) using RT-PCR and ligation-mediated PCR with primers designed based on conserved amino acid sequences of ERS from rice and corn. The GZ-ERS gene was 2,243 base pairs in length and coded for a polypeptide of 633 amino acid residues. The amino acid sequence of GZ-ERS encoded protein was 97, 96 and 91 % identical to OS-ERS (AAX95525) of rice, ZmERS25 (AAR25567) of corn, and Sc-ERS (ACF60981) of a sugarcane cv. ROC22, respectively. Genomic Southern analysis using a GZ-ERS cDNA insert as the probe indicated the presence of a single copy of GZ-ERS gene per haploid sugarcane genome. Real-time qPCR analysis showed that GZ-ERS was widely expressed in the vegetative tissues of sugarcane, and its expression in maturing internodes and leaf tissues was higher than in immature tissues. Furthermore, the expression level of GZ-ERS was the highest in maturing leaves, but very low in immature internodes. The GZ-ERS expression level in maturing leaves was enhanced by ethephon treatment, but was reduced under low temperature, drought, and dark conditions. The results from this study may help understand the regulation of gene expression in sugarcane and its responses to plant growth regulator ethephon and environmental stresses. As the sugarcane ERS family has multiple members, other members need to be isolated and characterized in future studies. C1 [Wang, Ai-Qin; Yang, Li-Tao; Li, Yang-Rui] Minist Agr, Key Lab Sugarcane Biotechnol & Genet Improvement, Nanning 530007, Peoples R China. [Wang, Ai-Qin; Yang, Li-Tao; Li, Yang-Rui] Chinese Acad Agr Sci, Guangxi Acad Agr Sci, Sugarcane Res Ctr, Guangxi Key Lab Sugarcane Genet Improvement, Nanning 530007, Peoples R China. [Wang, Ai-Qin; Ye, Xing-Zhi; Huang, Jing-Li; Niu, Jun-Qi; Liu, Ming; Yang, Li-Tao; Li, Yang-Rui] Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China. [Pan, Yong-Bao] USDA ARS, Midsouth Area, Sugarcane Res Lab, Houma, LA 70360 USA. RP Li, YR (reprint author), Minist Agr, Key Lab Sugarcane Biotechnol & Genet Improvement, Nanning 530007, Peoples R China. EM yongbao.pan@ars.usda.gov; liyr@gxaas.net FU China National R & D Research Program Project [2007BAD30B00]; International Scientific Exchange Program Projects [2008DFA30600, 2009DFA30820]; Guangxi R & D Research Program Projects [0782004-3, 0815011]; Guangxi Natural Science Foundation Projects [2010GXNSFA013101, 2011GXNSFF018002] FX The authors would like to thank Beijing Proteome Research Center, China for MALDI TOF/TOF MS analysis and Perng-Kuang Chang, Duli Zhao, and Zhanguo Xin for reviewing the manuscript. This work was supported by China National R & D Research Program Project (2007BAD30B00), International Scientific Exchange Program Projects (2008DFA30600, 2009DFA30820), Guangxi R & D Research Program Projects (Gui Ke Gong 0782004-3, Gui Ke Neng 0815011) and Guangxi Natural Science Foundation Projects (2010GXNSFA013101, 2011GXNSFF018002). NR 24 TC 0 Z9 0 U1 2 U2 55 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0972-1525 J9 SUGAR TECH JI Sugar Tech. PD MAR PY 2015 VL 17 IS 1 BP 22 EP 30 DI 10.1007/s12355-014-0324-3 PG 9 WC Agronomy SC Agriculture GA AT2HF UT WOS:000344753800004 ER PT J AU Cook, D Oliveira, CA Gardner, DR Pfister, JA Riet-Correa, G Riet-Correa, F AF Cook, Daniel Oliveira, Carlos A. Gardner, Dale R. Pfister, James A. Riet-Correa, Gabriela Riet-Correa, Franklin TI Changes in swainsonine, calystegine, and nitrogen concentrations on an annual basis in Ipomoea carnea SO TOXICON LA English DT Article DE Ipomoea carnea; Swainsonine; Calystegines; Protein ID NORTHEASTERN BRAZIL; SUBSP FISTULOSA; GOATS; LIVESTOCK; LOCOWEED; SHEEP AB Ipomoea carnea, a swainsonine containing plant, is known to cause a neurologic disease in grazing livestock in Brazil and other parts of the world. To better understand the relative toxicity and nutritional content of I. cameo we investigated swainsonine, calystegine, and crude protein concentrations in leaves of I. cameo on a monthly basis for one year in northern and northeastern Brazil. Swainsonine concentrations were detected at concentrations that could potentially poison an animal throughout the year although there was some variation between months. At one location swainsonine concentrations were generally the highest during the rainy season or the months immediately following the rainy season. Total calystegine concentrations were similar to those reported previously while crude protein concentrations were similar to those found in other Ipomoea species and are such that they may explain why I. cornea becomes desirable to grazing livestock as forage becomes limited during the dry season. Published by Elsevier Ltd. C1 [Cook, Daniel; Gardner, Dale R.; Pfister, James A.] ARS, USDA, Poisonous Plant Res Lab, Logan, UT 84341 USA. [Oliveira, Carlos A.; Riet-Correa, Gabriela] Fed Univ Para UFPA, Cent Diagnost Vet, Escola Vet, BR-68743080 Castanhal, Para, Brazil. [Riet-Correa, Franklin] Fed Univ Campina Grande UFCG, Vet Hosp, BR-58700000 Patos de Minas, Paraiba, Brazil. RP Cook, D (reprint author), ARS, USDA, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov FU National Institute for Science and Technology for the Control of Plant Poisonings [573534/2008-0] FX We would like to thank Kermit Price, Scott Larsen and Jessie Roper for their technical assistance. This work was partially financially supported by the National Institute for Science and Technology for the Control of Plant Poisonings (Grant 573534/2008-0). NR 31 TC 2 Z9 3 U1 0 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD MAR PY 2015 VL 95 BP 62 EP 66 DI 10.1016/j.toxicon.2015.01.002 PG 5 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA CM5SU UT WOS:000357749700009 PM 25572338 ER PT J AU Starkenburg, D Fochesatto, GJ Cristobal, J Prakash, A Gens, R Alfieri, JG Nagano, H Harazono, Y Iwata, H Kane, DL AF Starkenburg, Derek Fochesatto, Gilberto J. Cristobal, Jordi Prakash, Anupma Gens, Rudiger Alfieri, Joseph G. Nagano, Hirohiko Harazono, Yoshinobu Iwata, Hiroki Kane, Douglas L. TI Temperature regimes and turbulent heat fluxes across a heterogeneous canopy in an Alaskan boreal forest SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article ID ENERGY-BALANCE CLOSURE; EDDY-COVARIANCE; CLIMATE SYSTEM; BOUNDARY-LAYER; SURFACE; UNCERTAINTIES; INTERIOR; EXCHANGE; TOWERS AB We evaluate local differences in thermal regimes and turbulent heat fluxes across the heterogeneous canopy of a black spruce boreal forest on discontinuous permafrost in interior Alaska. The data were taken during an intensive observing period in the summer of 2013 from two micrometeorological towers 600m apart in a central section of boreal forest, one in a denser canopy (DC) and the other in a sparser canopy, but under approximately similar atmospheric boundary layer (ABL) flow conditions. Results suggest that on average 34% of the half-hourly periods in a day are nonstationary, primarily during night and during ABL transitions. Also, thermal regimes differ between the two towers; specifically between midnight and 0500 Alaska Standard Time (AKST) it is about 3 degrees C warmer at DC. On average, the sensible heat flux at DC was greater. For midday periods, the difference between those fluxes exceeded 30% of the measured flux and over 30 W m(-2) in magnitude more than 60% of the time. These differences are due to higher mechanical mixing as a result of the increased density of roughness elements at DC. Finally, the vertical distribution of turbulent heat fluxes verifies a maximum atop the canopy crown (2.6 h) when compared with the subcanopy (0.6 h) and above canopy (5.1 h), where h is the mean canopy height. We argue that these spatial and vertical variations of sensible heat fluxes result from the complex scale aggregation of energy fluxes over a heterogeneous canopy. C1 [Starkenburg, Derek; Fochesatto, Gilberto J.] Univ Alaska Fairbanks, Inst Geophys, Dept Atmospher Sci, Fairbanks, AK 99775 USA. [Starkenburg, Derek; Fochesatto, Gilberto J.] Univ Alaska Fairbanks, Coll Nat Sci & Math, Fairbanks, AK USA. [Cristobal, Jordi; Prakash, Anupma; Gens, Rudiger] Univ Alaska Fairbanks, Inst Geophys, Fairbanks, AK 99775 USA. [Alfieri, Joseph G.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Nagano, Hirohiko; Harazono, Yoshinobu; Iwata, Hiroki] Univ Alaska Fairbanks, Int Arctic Res Ctr, Fairbanks, AK USA. [Iwata, Hiroki] Shinshu Univ, Fac Sci, Nagano, Japan. [Kane, Douglas L.] Univ Alaska Fairbanks, Water Environm Res Ctr, Fairbanks, AK USA. RP Starkenburg, D (reprint author), Univ Alaska Fairbanks, Inst Geophys, Dept Atmospher Sci, Fairbanks, AK 99775 USA. EM dpstarkenburg@alaska.edu RI Cristobal Rossello, Jordi/K-8021-2013; Nagano, Hirohiko/K-8145-2012; Iwata, Hiroki/B-7679-2008; OI Cristobal Rossello, Jordi/0000-0001-6244-4289; Nagano, Hirohiko/0000-0002-3090-7709; FOCHESATTO, Gilberto J/0000-0003-0813-6765 FU Alaska NASA EPSCoR program [NNX10NO2A]; Alaska Space Grant Program; NSF [DGE-0948029] FX This research was supported by the Alaska NASA EPSCoR program award NNX10NO2A, by the Alaska Space Grant Program, and by the "New GK-12 Program: The CASE (Changing Alaska Science Education) for Enhancing Understanding of Climate Change" NSF (DGE-0948029). Data sets for this paper can be requested from G.J. Fochesatto (foch@gi.alaska.edu), Geophysical Institute University of Alaska Fairbanks, for DC and Doppler sodar sites, and from Y. Harazono (yharazono@iarc.uaf.edu), International Arctic Research Center University of Alaska Fairbanks, for SC site. NR 44 TC 3 Z9 3 U1 0 U2 3 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD FEB 27 PY 2015 VL 120 IS 4 BP 1348 EP 1360 DI 10.1002/2014JD022338 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CD6EN UT WOS:000351182400008 ER PT J AU Naoumkina, M Thyssen, GN Fang, DD AF Naoumkina, Marina Thyssen, Gregory N. Fang, David D. TI RNA-seq analysis of short fiber mutants Ligon-lintless-1 (Li-1) and-2 (Li-2) revealed important role of aquaporins in cotton (Gossypium hirsutum L.) fiber elongation SO BMC PLANT BIOLOGY LA English DT Article ID LIGON LINTLESS-2 MUTANT; UPLAND COTTON; TONOPLAST AQUAPORIN; CELL ELONGATION; EXPRESSION; GROWTH; GENOMICS; ROOT; LINKAGE; PLANT AB Background: Cotton fiber length is a key determinant of fiber quality for the textile industry. Understanding the molecular basis of fiber elongation would provide a means for improvement of fiber length. Ligon lintless-1 (Li-1) and Ligon lintless-2 (Li-2) are monogenic and dominant mutations, that result in an extreme reduction in the length of lint fiber to approximately 6 mm on mature seeds. In a near-isogenic state with wild type (WT) cotton these two short fiber mutants provide an excellent model system to study mechanisms of fiber elongation. Results: We used next generation sequencing (RNA-seq) to identify common fiber elongation related genes in developing fibers of Li-1 and Li-2 mutants growing in the field and a greenhouse. We found a large number of differentially expressed genes common to both mutants, including 531 up-regulated genes and 652 down-regulated genes. Major intrinsic proteins or aquaporins were one of the most significantly over-represented gene families among common down-regulated genes in Li-1 and Li-2 fibers. The members of three subfamilies of aquaporins, including plasma membrane intrinsic proteins, tonoplast intrinsic proteins and NOD26-like intrinsic proteins were down-regulated in short fiber mutants. The osmotic concentration and the concentrations of soluble sugars were lower in fiber cells of both short fiber mutants than in WT, whereas the concentrations of K+ and malic acid were significantly higher in mutants during rapid cell elongation. Conclusions: We found that the aquaporins were the most down-regulated gene family in both short fiber mutants. The osmolality and concentrations of soluble sugars were less in saps of Li-1 - Li-2, whereas the concentrations of malic acid, K+ and other detected ions were significantly higher in saps of mutants than in WT. These results suggest that higher accumulation of ions in fiber cells, reduced osmotic pressure and low expression of aquaporins, may contribute to the cessation of fiber elongation in Li-1 and Li-2 short-fiber mutants. The research presented here provides new insights into osmoregulation of short fiber mutants and the role of aquaporins in cotton fiber elongation. C1 [Naoumkina, Marina; Thyssen, Gregory N.; Fang, David D.] USDA ARS SRRC, Cotton Fiber Biosci Res Unit, New Orleans, LA 70124 USA. RP Naoumkina, M (reprint author), USDA ARS SRRC, Cotton Fiber Biosci Res Unit, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM marina.naoumkina@ars.usda.gov FU USDA-ARS CRIS project [6435-21000-0017D]; USDA-ARS Cotton Incorporated project [12-210] FX This project was financially supported by the USDA-ARS CRIS project # 6435-21000-0017D and Cotton Incorporated project # 12-210. We greatly appreciate the contributions of Christopher Florane from our group for assistance with sample collection and nucleic acid extraction and Danielle Lee Burnett from commodity utilization unit for running ICP-OES. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture that is an equal opportunity provider and employer. NR 47 TC 5 Z9 6 U1 1 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD FEB 27 PY 2015 VL 15 AR 65 DI 10.1186/s12870-015-0454-0 PG 14 WC Plant Sciences SC Plant Sciences GA CD1SH UT WOS:000350854100001 PM 25848981 ER PT J AU Wisniewski, M Norelli, J Artlip, T AF Wisniewski, Michael Norelli, John Artlip, Timothy TI Oyerexpression of a peach CBF gene in apple: a model for understanding the integration of growth, dormancy, and cold hardiness in woody plants SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE freezing tolerance; fruit trees; DAM genes; CBF genes; bud break; DELLA genes; EBB genes; Malus x domestica ID MADS-BOX GENES; ENHANCES FREEZING TOLERANCE; PERSICA L. BATSCH; LOW-TEMPERATURE; TRANSCRIPTION FACTORS; REGULATORY ELEMENTS; SIGNALING PATHWAY; DELLA PROTEINS; VITIS-VINIFERA; BUD FORMATION AB The timing of cold acclimation and deacclimation, dormancy, and budbreak play an integral role in the life cycle of woody plants. The molecular events that regulate these parameters have been the subject of much study, however, in most studies these events have been investigated independently of each other. Ectopic expression of a peach CBF (PpCBF1) in apple increases the level of both non-acclimated and acclimated freezing tolerance relative to the non-transformed control, and also inhibits growth, induces early bud set and leaf senescence, and delays bud break in the spring. The current study examined differences in the seasonal expression of genes (CBE DAM, RGL, and EBB) that have been reported to be associated with freezing tolerance, dormancy, growth, and bud break, respectively, in the PpCBF1 T166 transgenic apple line and the non-transformed M.26 control. Results indicated that expression of several of these key genes, including MdDAM, MdRGL, and MdEBB was altered in transgenic T166 trees relative to non-transformed M.26 trees. In particular, several putative MdDAM genes, associated with the dormancy-cycle in other species of woody plants in the Rosaceae, exhibited different patterns of expression in the T166 vs. M.26 trees. Additionally, for the first time a putative APETALA2/Ethylene-responsive transcription factor, originally described in poplar and shown to regulate the timing of bud break, was shown to be associated with the timing of bud break in apple. Since the overexpression of PpCBF1 in apple results in a dramatic alteration in cold acclimation, dormancy, and growth, this transgenic line (T166) may represent a useful model for studying the integration of these seasonal life-cycle parameters. C1 [Wisniewski, Michael; Norelli, John; Artlip, Timothy] USDA ARS, Kearneysville, WV 25430 USA. RP Wisniewski, M (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.wisniewski@ars.usda.gov NR 56 TC 12 Z9 12 U1 13 U2 53 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 27 PY 2015 VL 6 AR 85 DI 10.3389/fpls.2015.00085 PG 13 WC Plant Sciences SC Plant Sciences GA CD1AE UT WOS:000350805200001 PM 25774159 ER PT J AU Esteve-Gassent, MD Grover, A Feria-Arroyo, TP Castro-Arellano, I Medina, RF Gordillo-Perez, G de Leon, AAP AF Esteve-Gassent, Maria D. Grover, Abha Feria-Arroyo, Teresa P. Castro-Arellano, Ivan Medina, Raul F. Gordillo-Perez, Guadalupe de Leon, Adalberto A. Perez TI Prevalence of Borrelia burgdorferi-infected ticks from wildlife hosts, a response to Norris et al. SO PARASITES & VECTORS LA English DT Letter ID EASTERN UNITED-STATES; LYME-DISEASE; IXODES-SCAPULARIS; MEXICO; DENSITY; VECTOR; MODELS; AGENTS; TEXAS; RISK AB In a recent Letter to the Editor, Norris et al. questioned the validity of some of our data reported by Feria-Arroyo et al. The main issue investigated by us was the potential impact of climate change on the probable distribution of the tick vector Ixodes scapularis in the Texas-Mexico transboundary region. As an ancillary issue, an analysis of sequence data for the intergenic spacer of Borrelia burgdorferi was conducted. In the present letter, we provide further evidence supporting our original results, and advocate that extensive study of the population genetics of B. burgdorferi is needed in the Texas-Mexico transboundary region. C1 [Esteve-Gassent, Maria D.; Grover, Abha] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. [Feria-Arroyo, Teresa P.] Univ Texas Pan Amer, Dept Biol, Edinburg, TX 78539 USA. [Castro-Arellano, Ivan] SW Texas State Univ, Coll Sci & Engn, Dept Biol, San Marcos, TX 78666 USA. [Medina, Raul F.] Texas A&M Univ, Coll Agr & Life Sci, Dept Entomol, College Stn, TX 77843 USA. [Gordillo-Perez, Guadalupe] Ctr Med Nacl SXXI, IMSS, Unidad Invest Enfermedades Infecciosas, Mexico City 06720, DF, Mexico. [de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Esteve-Gassent, MD (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. EM MEsteve-Gassent@cvm.tamu.edu NR 19 TC 1 Z9 2 U1 4 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 27 PY 2015 VL 8 AR 129 DI 10.1186/s13071-015-0739-z PG 7 WC Parasitology SC Parasitology GA CD4GH UT WOS:000351040100001 PM 25885773 ER PT J AU Coates, BS AF Coates, Brad S. TI Horizontal transfer of a non-autonomous Helitron among insect and viral genomes SO BMC GENOMICS LA English DT Article DE Horizontal transposon transfer; Viral vectors ID ROLLING-CIRCLE TRANSPOSONS; DROSOPHILA-MELANOGASTER; MYOTIS-LUCIFUGUS; SERIAL PASSAGE; DNA TRANSPOSON; GENE-TRANSFER; ELEMENTS; MAIZE; EVOLUTION; SEQUENCE AB Background: The movement of mobile elements among species by horizontal transposon transfer (HTT) influences the evolution of genomes through the modification of structure and function. Helitrons are a relatively new lineage of DNA-based (class II) transposable elements (TEs) that propagate by rolling-circle replication, and are capable of acquiring host DNA. The rapid spread of Helitrons among animal lineages by HTT is facilitated by shuttling in viral particles or by unknown mechanisms mediated by close organism associations (e.g. between hosts and parasites). Results: A non-autonomous Helitron independently annotated as BmHel-2 from Bombyx mori and the MITE01 element from Ostrinia nubilalis was predicted in the genomes of 24 species in the insect Order Lepidoptera. Integrated Helitrons retained >= 65% sequence identity over a 250 bp consensus, and were predicted to retain secondary structures inclusive of a 3'-hairpin and a 5'-subterminal inverted repeat. Highly similar Hel-2 copies were predicted in the genomes of insects and associated viruses, which along with a previous documented case of real-time virus-insect cell line transposition suggests that this Helitron has likely propagated by HTT. Conclusions: These findings provide evidence that insect virus may mediate the HTT of Helitron-like TEs. This movement may facilitate the shuttling of DNA elements among insect genomes. Further sampling is required to determine the putative role of HTT in insect genome evolution. C1 [Coates, Brad S.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Coates, Brad S.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. RP Coates, BS (reprint author), Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. EM brad.coates@ars.usda.gov FU United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS) [3625-22000-017-00]; Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA [3543]; USDA-ARS Congressional Appropriated funds [NP304] FX This research was a joint contribution from the United States Department of Agriculture (USDA), Agricultural Research Service (ARS) (CRIS Project 3625-22000-017-00), and the Iowa Agriculture and Home Economics Experiment Station, Iowa State University, Ames, IA (Project 3543). Funding was by USDA-ARS Congressional Appropriated funds, NP304 Crop Protection and Quarantine, Project Tile: Ecologically-based Management of Insect Pests of Corn, with Emphasis on Corn Borers, Rootworms, and Cutworms. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by USDA for its use. USDA is an equal opportunity employer and provider. NR 59 TC 5 Z9 6 U1 4 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 27 PY 2015 VL 16 AR 137 DI 10.1186/s12864-015-1318-6 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CC2IL UT WOS:000350168600001 PM 25766741 ER PT J AU Oellrich, A Walls, RL Cannon, EKS Cannon, SB Cooper, L Gardiner, J Gkoutos, GV Harper, L He, MZ Hoehndorf, R Jaiswal, P Kalberer, SR Lloyd, JP Meinke, D Menda, N Moore, L Nelson, RT Pujar, A Lawrence, CJ Huala, E AF Oellrich, Anika Walls, Ramona L. Cannon, Ethalinda K. S. Cannon, Steven B. Cooper, Laurel Gardiner, Jack Gkoutos, Georgios V. Harper, Lisa He, Mingze Hoehndorf, Robert Jaiswal, Pankaj Kalberer, Scott R. Lloyd, John P. Meinke, David Menda, Naama Moore, Laura Nelson, Rex T. Pujar, Anuradha Lawrence, Carolyn J. Huala, Eva TI An ontology approach to comparative phenomics in plants SO PLANT METHODS LA English DT Article ID BIOMEDICAL ONTOLOGIES; GENE ONTOLOGY; MEDICAGO-TRUNCATULA; INFORMATION; PHENOTYPES; DATABASE; PATHWAY; ARABIDOPSIS; EVOLUTION; TOOL AB Background: Plant phenotype datasets include many different types of data, formats, and terms from specialized vocabularies. Because these datasets were designed for different audiences, they frequently contain language and details tailored to investigators with different research objectives and backgrounds. Although phenotype comparisons across datasets have long been possible on a small scale, comprehensive queries and analyses that span a broad set of reference species, research disciplines, and knowledge domains continue to be severely limited by the absence of a common semantic framework. Results: We developed a workflow to curate and standardize existing phenotype datasets for six plant species, encompassing both model species and crop plants with established genetic resources. Our effort focused on mutant phenotypes associated with genes of known sequence in Arabidopsis thaliana (L.) Heynh. (Arabidopsis), Zea mays L. subsp. mays (maize), Medicago truncatula Gaertn. (barrel medic or Medicago), Oryza sativa L. (rice), Glycine max (L.) Merr. (soybean), and Solanum lycopersicum L. (tomato). We applied the same ontologies, annotation standards, formats, and best practices across all six species, thereby ensuring that the shared dataset could be used for cross-species querying and semantic similarity analyses. Curated phenotypes were first converted into a common format using taxonomically broad ontologies such as the Plant Ontology, Gene Ontology, and Phenotype and Trait Ontology. We then compared ontology-based phenotypic descriptions with an existing classification system for plant phenotypes and evaluated our semantic similarity dataset for its ability to enhance predictions of gene families, protein functions, and shared metabolic pathways that underlie informative plant phenotypes. Conclusions: The use of ontologies, annotation standards, shared formats, and best practices for cross-taxon phenotype data analyses represents a novel approach to plant phenomics that enhances the utility of model genetic organisms and can be readily applied to species with fewer genetic resources and less well-characterized genomes. In addition, these tools should enhance future efforts to explore the relationships among phenotypic similarity, gene function, and sequence similarity in plants, and to make genotype-to-phenotype predictions relevant to plant biology, crop improvement, and potentially even human health. C1 [Oellrich, Anika] Wellcome Trust Sanger Inst, Hinxton CB10 1SA, England. [Walls, Ramona L.] Univ Arizona, iPlant Collaborat, Tucson, AZ 85721 USA. [Cannon, Ethalinda K. S.] Iowa State Univ, Dept Elect & Comp Engn, Ames, IA 50011 USA. [Cannon, Steven B.; Nelson, Rex T.] Iowa State Univ, USDA ARS, Crop Genome Informat Lab, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Cannon, Steven B.; Lawrence, Carolyn J.] Iowa State Univ, Dept Agron, Ames, IA 50010 USA. [Cooper, Laurel; Moore, Laura] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gardiner, Jack; Lawrence, Carolyn J.] Iowa State Univ, Dept Genet Dev & Cell Biol, Roy J Carver Co Lab, Ames, IA 50010 USA. [Gkoutos, Georgios V.] Aberystwyth Univ, Dept Comp Sci, Aberystwyth SY23 3FG, Dyfed, Wales. [Hoehndorf, Robert] King Abdullah Univ Sci & Technol, Comp Elect & Math Sci & Engn Div, Thuwal 239556900, Saudi Arabia. [Hoehndorf, Robert] King Abdullah Univ Sci & Technol, Computat Biosci Res Ctr, Thuwal 239556900, Saudi Arabia. [Lloyd, John P.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Meinke, David] Oklahoma State Univ, Dept Bot, Stillwater, OK 74078 USA. [Pujar, Anuradha] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Huala, Eva] Phoenix Bioinformat, Redwood City, CA 94063 USA. RP Lawrence, CJ (reprint author), Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50010 USA. EM triffid@iastate.edu; huala@phoenixbioinformatics.org RI Jaiswal, Pankaj/H-7599-2016; OI Jaiswal, Pankaj/0000-0002-1005-8383; Hoehndorf, Robert/0000-0001-8149-5890 FU National Science Foundation [DBI-0735191, DBI-1265383, IOS-0822201, IOS-1127112, IOS-1340112]; NSF Phenotype RCN FX The NSF Phenotype RCN supported this work by allowing plant and computational biologists to come together and develop a common vocabulary and supported the preliminary analysis of generated data. The Samuel Roberts Noble Foundation provided the Cyc database for Medicago (MedicCyc version 2.0). RLW was supported in part by the National Science Foundation under Grant Numbers #DBI-0735191 and #DBI-1265383. PJ and LC contributions are supported by the National Science Foundation Awards #IOS-0822201, #IOS-1127112 and #IOS-1340112. NR 59 TC 6 Z9 7 U1 1 U2 26 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-4811 J9 PLANT METHODS JI Plant Methods PD FEB 25 PY 2015 VL 11 AR 10 DI 10.1186/s13007-015-0053-y PG 15 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA CD1TL UT WOS:000350857100001 PM 25774204 ER PT J AU Cheng, CX Jiao, C Singer, SD Gao, M Xu, XZ Zhou, YM Li, Z Fei, ZJ Wang, YJ Wang, XP AF Cheng, Chenxia Jiao, Chen Singer, Stacy D. Gao, Min Xu, Xiaozhao Zhou, Yiming Li, Zhi Fei, Zhangjun Wang, Yuejin Wang, Xiping TI Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca x V-vinifera) cv. Kyoho flowers SO BMC GENOMICS LA English DT Article DE Grape; Gibberellic acid; Gene expression; Pathways; RNA-seq; Transcriptome ID PHENYLALANINE AMMONIA-LYASE; MOLECULAR-GENETIC ANALYSIS; STAMEN DEVELOPMENT; ABSCISIC-ACID; SIGNAL-TRANSDUCTION; SALICYLIC-ACID; MALE-STERILE; RNA-SEQ; ARABIDOPSIS; BIOSYNTHESIS AB Background: Gibberellins are well known for their growth control function in flower, fruit and seed development, and as such, exogenous gibberellic acid (GA) application plays an important role in viticulture. Unfortunately, the mechanism by which GA(3) acts in the regulation of these complicated developmental processes in grape remains unclear. Results: In the present study, we demonstrated that application of GA(3) to 'Kyoho' grapevine inflorescences at pre-bloom promoted flower opening, and induced fruit coloring as well as seed abortion. In an attempt to obtain a deeper understanding of the molecular mechanisms driving these responses to GA(3) treatment, we performed large-scale transcriptome sequencing of grape flowers following GA(3) treatment using Illumina sequencing technology. Global expression profiles of GA(3)-treated and untreated grape flowers were compared and a large number of GA(3)-responsive genes were identified. Gene ontology (GO) term classification and biochemical pathway analyses indicated that GA(3) treatment caused changes in the levels of transcripts involved in cellular processes, reproduction, hormone and secondary metabolism, as well as the scavenging and detoxification of reactive oxygen species (ROS). These findings suggest that GA(3)-induced morphological alterations may be related to the control of hormone biosynthesis and signaling, regulation of transcription factors, alteration of secondary metabolites, and the stability of redox homeostasis. Conclusions: Taken together, this comprehensive inflorescence transcriptome data set provides novel insight into the response of grape flowers to GA(3) treatment, and also provides possible candidate genes or markers that could be used to guide future efforts in this field. C1 [Cheng, Chenxia; Jiao, Chen; Gao, Min; Zhou, Yiming; Li, Zhi; Wang, Yuejin; Wang, Xiping] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Cheng, Chenxia; Jiao, Chen; Gao, Min; Li, Zhi; Wang, Yuejin; Wang, Xiping] Northwest A&F Univ, Key Lab Hort Plant Biol & Germplasm Innovat North, Minist Agr, Yangling 712100, Shaanxi, Peoples R China. [Cheng, Chenxia] China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China. [Singer, Stacy D.] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. [Xu, Xiaozhao] China Agr Univ, Inst Hort Plants, Beijing 100193, Peoples R China. [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Wang, XP (reprint author), Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. EM wangxiping@nwsuaf.edu.cn FU National Natural Science Foundation of China [31272136]; 948 Project from Ministry of Agriculture of China [2012-S12]; Program for Innovative Research Team of Grape Germplasm Resources and Breeding [2013KCT-25] FX This work was supported by the National Natural Science Foundation of China (31272136), 948 Project from Ministry of Agriculture of China (2012-S12), as well as the Program for Innovative Research Team of Grape Germplasm Resources and Breeding (2013KCT-25). NR 74 TC 6 Z9 8 U1 6 U2 51 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 25 PY 2015 VL 16 AR 128 DI 10.1186/s12864-015-1324-8 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CC5ER UT WOS:000350382400001 PM 25888129 ER PT J AU Zeller, WE Ramsay, A Ropiak, HM Fryganas, C Mueller-Harvey, I Brown, RH Drake, C Grabber, JH AF Zeller, Wayne E. Ramsay, Aina Ropiak, Honorata M. Fryganas, Christos Mueller-Harvey, Irene Brown, Ronald H. Drake, Chris Grabber, John H. TI H-1-C-13 HSQC NMR Spectroscopy for Estimating Procyanidin/Prodelphinidin and cis/trans-Flavan-3-ol Ratios of Condensed Tannin Samples: Correlation with Thiolysis SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE condensed tannins; proanthocyanidins; procyanidins; prodelphinidins; nuclear magnetic resonance spectroscopy; NMR; thiolysis ID NUCLEAR-MAGNETIC-RESONANCE; C-13 NMR; TEMPERATE FORAGES; PROANTHOCYANIDINS; RUMINANTS; NUTRITION; POLARIZATION; PROCYANIDINS; ELUCIDATION; HEALTH AB Studies with a diverse array of 22 purified condensed tannin (CT) samples from nine plant species demonstrated that procyanidin/prodelphinidin (PC/PD) and cis/trans-flavan-3-ol ratios can be appraised by H-1-C-13 HSQC NMR spectroscopy. The method was developed from samples containing 44-similar to 100% CT, PC/PD ratios ranging from 0/100 to 99/1, and cis/trans ratios ranging from 58/42 to 95/5 as determined by thiolysis with benzyl mercaptan. Integration of cross-peak contours of H/C-6' signals from PC and of H/C-2',6' signals from PD yielded nuclei-adjusted estimates that were highly correlated with PC/PD ratios obtained by thiolysis (R-2 = 0.99). cis/trans-Flavan-3-ol ratios, obtained by integration of the respective H/C-4 cross-peak contours, were also related to determinations made by thiolysis (R-2 = 0.89). Overall, H-1-C-13 HSQC NMR spectroscopy appears to be a viable alternative to thiolysis for estimating PC/PD and cis/trans ratios of CT if precautions are taken to avoid integration of cross-peak contours of contaminants. C1 [Zeller, Wayne E.; Grabber, John H.] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. [Ramsay, Aina; Ropiak, Honorata M.; Fryganas, Christos; Mueller-Harvey, Irene; Brown, Ronald H.; Drake, Chris] Univ Reading, Sch Agr Policy & Dev, Food Prod & Qual Div, Chem & Biochem Lab, Reading RG6 6AT, Berks, England. RP Zeller, WE (reprint author), ARS, US Dairy Forage Res Ctr, USDA, 1925 Linden Dr West, Madison, WI 53706 USA. EM wayne.zeller@ars.usda.gov FU USDA-ARS [58-3655-0-155F]; University of Reading, UK; European Union Marie Curie Initial Training Network [PITN-GA-2011-289377] FX This work was funded in part by a USDA-ARS specific cooperative agreement no. 58-3655-0-155F with the University of Reading, UK, and was supported by a European Union Marie Curie Initial Training Network (PITN-GA-2011-289377, "LegumePlus"). Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 39 TC 5 Z9 5 U1 4 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 25 PY 2015 VL 63 IS 7 BP 1967 EP 1973 DI 10.1021/jf504743b PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CC2RX UT WOS:000350193400009 PM 25629428 ER PT J AU Davis, TZ Lee, ST Collett, MG Stegelmeier, BL Green, BT Buck, SR Pfister, JA AF Davis, T. Zane Lee, Stephen T. Collett, Mark G. Stegelmeier, Bryan L. Green, Benedict T. Buck, Steven R. Pfister, James A. TI Toxicity of White Snakeroot (Ageratina altissima) and Chemical Extracts of White Snakeroot in Goats SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE white Snakeroot; tremetone; tremetol; Ageratina altissima; Eupatorium rugosum; trembles; milk sickness; benzofuran ketones; goats ID GOLDENROD ISOCOMA-PLURIFLORA; EUPATORIUM URTICAEFOLIUM; MILK SICKNESS; CONSTITUENTS; TREMETONE; KETONES AB White snakeroot (Ageratina altissima) is a sporadically toxic plant that causes trembles in livestock and milk sickness in humans that drink tainted milk. The putative toxin in white snakeroot is tremetone and possibly other benzofuran ketones, even though it has not been demonstrated in vivo. Toxic white snakeroot was dosed to goats, and they developed clinical signs of poisoning, exercise intolerance, significant increases in serum enzyme activities, and histological changes. Tremetone and the other benzofuran ketones were extracted with hexane; the extracts and residues were analyzed for tremetone and dosed to goats at tremetone and benzofuran ketone concentrations similar to the original plant material. However, none of the dosed goats developed the disease. The results demonstrate for the first time that white snakeroot is a potent myotoxin in goats and that other compound(s), which may be lost or modified during the extraction process, could be involved in causing trembles and milk sickness. C1 [Davis, T. Zane; Lee, Stephen T.; Stegelmeier, Bryan L.; Green, Benedict T.; Pfister, James A.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Collett, Mark G.] Massey Univ, Inst Vet Anim & Biomed Sci, Palmerston North 4442, Manawatu, New Zealand. [Buck, Steven R.] Univ Illinois, Champaign, IL 61820 USA. RP Davis, TZ (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM zane.davis@ars.usda.gov FU USDA/ARS FX This research was supported by USDA/ARS. NR 14 TC 2 Z9 2 U1 2 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 25 PY 2015 VL 63 IS 7 BP 2092 EP 2097 DI 10.1021/jf505614z PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CC2RX UT WOS:000350193400023 PM 25641641 ER PT J AU Sugimoto, N Forsline, P Beaudry, R AF Sugimoto, Nobuko Forsline, Philip Beaudry, Randolph TI Volatile Profiles of Members of the USDA Geneva Malus Core Collection: Utility in Evaluation of a Hypothesized Biosynthetic Pathway for Esters Derived from 2-Methylbutanoate and 2-Methylbutan-1-ol SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE ester; citramalate; isoleucine; threonine; aroma; flavor ID ALCOHOL ACYL-TRANSFERASE; DELICIOUS APPLE FRUIT; SACCHAROMYCES-CEREVISIAE; ISOLEUCINE BIOSYNTHESIS; THREONINE DEAMINASE; BRANCHED-CHAIN; AMINO-ACIDS; ESCHERICHIA-COLI; DOMESTICA BORKH.; FLAVOR COMPOUNDS AB The volatile ester and alcohol profiles of ripening apple fruit from 184 germplasm lines in the USDA Malus Germplasm Repository at the New York Agricultural Experiment Station in Geneva, NY, USA, were evaluated. Cluster analysis suggested biochemical relationships exist between several ester classes. A strong linkage was revealed between 2-methylbutanoate, propanoate, and butanoate esters, suggesting the influence of the recently proposed "citramalic acid pathway" in apple fruit. Those lines with a high content of esters formed from 2-methylbutan-1-ol and 2-methylbutanoate (2MB) relative to straight-chain (SC) esters (high 2MB/SC ratio) exhibited a marked increase in isoleucine and citramalic acid during ripening, but those lines with a low content did not. Thus, the data were consistent with the existence of the hypothesized citramalic acid pathway and suggest that the Geneva Malus Germplasm Repository, appropriately used, could be helpful in expanding our understanding of mechanisms for fruit volatile synthesis and other aspects of secondary metabolism. C1 [Sugimoto, Nobuko; Beaudry, Randolph] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Forsline, Philip] Cornell Univ, Plant Genet Resources Unit, ARS, USDA, Geneva, NY 14456 USA. RP Beaudry, R (reprint author), Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. EM beaudry@msu.edu FU USDA-ARS Crop Germplasm Improvement Program; Michigan State University AgBioResearch FX We acknowledge financial support from the USDA-ARS Crop Germplasm Improvement Program and Michigan State University AgBioResearch. NR 87 TC 0 Z9 0 U1 1 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 25 PY 2015 VL 63 IS 7 BP 2106 EP 2116 DI 10.1021/jf505523m PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CC2RX UT WOS:000350193400025 PM 25650784 ER PT J AU Farfan, IDB De La Fuente, GN Murray, SC Isakeit, T Huang, PC Warburton, M Williams, P Windham, GL Kolomiets, M AF Farfan, Ivan D. Barrero De La Fuente, Gerald N. Murray, Seth C. Isakeit, Thomas Huang, Pei-Cheng Warburton, Marilyn Williams, Paul Windham, Gary L. Kolomiets, Mike TI Genome Wide Association Study for Drought, Aflatoxin Resistance, and Important Agronomic Traits of Maize Hybrids in the Sub-Tropics SO PLOS ONE LA English DT Article ID MULTIPLE-TESTING CORRECTION; GENERATION VARIETY TRIALS; PINUS-TAEDA L.; TROPICAL MAIZE; GRAIN-YIELD; ASPERGILLUS-FLAVUS; NONSTRESS ENVIRONMENTS; GERMPLASM LINES; COMPLEX TRAITS; PLANT HEIGHT AB The primary maize (Zea mays L.) production areas are in temperate regions throughout the world and this is where most maize breeding is focused. Important but lower yielding maize growing regions such as the sub-tropics experience unique challenges, the greatest of which are drought stress and aflatoxin contamination. Here we used a diversity panel consisting of 346 maize inbred lines originating in temperate, sub-tropical and tropical areas testcrossed to stiff-stalk line Tx714 to investigate these traits. Testcross hybrids were evaluated under irrigated and non-irrigated trials for yield, plant height, ear height, days to anthesis, days to silking and other agronomic traits. Irrigated trials were also inoculated with Aspergillus flavus and evaluated for aflatoxin content. Diverse maize testcrosses out-yielded commercial checks in most trials, which indicated the potential for genetic diversity to improve sub-tropical breeding programs. To identify genomic regions associated with yield, aflatoxin resistance and other important agronomic traits, a genome wide association analysis was performed. Using 60,000 SNPs, this study found 10 quantitative trait variants for grain yield, plant and ear height, and flowering time after stringent multiple test corrections, and after fitting different models. Three of these variants explained 5-10% of the variation in grain yield under both water conditions. Multiple identified SNPs co-localized with previously reported QTL, which narrows the possible location of causal polymorphisms. Novel significant SNPs were also identified. This study demonstrated the potential to use genome wide association studies to identify major variants of quantitative and complex traits such as yield under drought that are still segregating between elite inbred lines. C1 [Farfan, Ivan D. Barrero; De La Fuente, Gerald N.; Murray, Seth C.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Isakeit, Thomas; Huang, Pei-Cheng; Kolomiets, Mike] Texas A&M Univ, Dept Plant Pathol, College Stn, TX USA. [Warburton, Marilyn; Williams, Paul; Windham, Gary L.] ARS, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS USA. RP Murray, SC (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM sethmurray@tamu.edu FU USDA-NIFA-AFRI [2010-85117-20539] FX This work was supported by USDA-NIFA-AFRI. #2010-85117-20539: Improving Drought Tolerance and Aflatoxin Resistance in Maize; Education, Extension, and Translational Breeding via Altered Lipid Metabolism. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 128 TC 8 Z9 9 U1 2 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 25 PY 2015 VL 10 IS 2 AR e0117737 DI 10.1371/journal.pone.0117737 PG 30 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC2IM UT WOS:000350168700036 PM 25714370 ER PT J AU Kapczynski, DR Esaki, M Dorsey, KM Jiang, HJ Jackwood, M Moraes, M Gardin, Y AF Kapczynski, Darrell R. Esaki, Motoyuki Dorsey, Kristi M. Jiang, Haijun Jackwood, Mark Moraes, Mauro Gardin, Yannick TI Vaccine protection of chickens against antigenically diverse H5 highly pathogenic avian influenza isolates with a live HVT vector vaccine expressing the influenza hemagglutinin gene derived from a clade 2.2 avian influenza virus SO VACCINE LA English DT Article DE Highly pathogenic avian influenza; Turkey herpesvirus; Poultry; Vector-based vaccine; Antibodies Cellular immunity ID MAREKS-DISEASE VACCINES; HONG-KONG; EFFICACY; IMMUNITY; IDENTIFICATION; ANTIBODIES; CHALLENGE; INFECTION; OUTBREAK; POULTRY AB Vaccination is an important tool in the protection of poultry against avian influenza (AI). For field use, the overwhelming majority of AI vaccines produced are inactivated whole virus formulated into an oil emulsion. However, recombinant vectored vaccines are gaining use for their ability to induce protection against heterologous isolates and ability to overcome maternal antibody interference. In these studies, we compared protection of chickens provided by a turkey herpesvirus (HVT) vector vaccine expressing the hemagglutinin (HA) gene from a clade 2.2 H5N1 strain (A/swan/Hungary/4999/2006) against homologous H5N1 as well as heterologous H5N1 and H5N2 highly pathogenic (HP) AI challenge. The results demonstrated all vaccinated birds were protected from clinical signs of disease and mortality following homologous challenge. In addition, oral and cloaca] swabs taken from challenged birds demonstrated that vaccinated birds had lower incidence and titers of viral shedding compared to sham-vaccinated birds. Following heterologous H5N1 or H5N2 HPAI challenge, 80-95% of birds receiving the HVT vector AI vaccine at day of age survived challenge with fewer birds shedding virus after challenge than sham vaccinated birds. In vitro cytotoxicity analysis demonstrated that splenic T lymphocytes from HVT-vector-AI vaccinated chickens recognized MHC-matched target cells infected with H5, as well as H6, H7, or H9 AI virus. Taken together, these studies provide support for the use of HVT vector vaccines expressing HA to protect poultry against multiple lineages of HPAI, and that both humoral and cellular immunity induced by live vaccines likely contributes to protection. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license C1 [Kapczynski, Darrell R.; Jiang, Haijun] USDA ARS, Exot & Emerging Avian Dis Res Unit, SE Poultry Res Lab, Athens, GA 30605 USA. [Esaki, Motoyuki; Dorsey, Kristi M.; Moraes, Mauro] Ceva Anim Hlth, Lenexa, KS USA. [Jackwood, Mark] Univ Georgia, Dept Populat Hlth, Coll Vet Med, Athens, GA USA. [Gardin, Yannick] Ceva Anim Hlth, Libourne, France. RP Kapczynski, DR (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM darrell.kapczynski@ars.usda.gov OI Esaki, Motoyuki/0000-0003-4023-2407 FU USDA, ARS CRIS [6612-480-062]; [193-6612-911] FX The authors wish to thank Aniko Zsak, Cam Greene and Debbie Hilt for technical assistance, and Roger Brock for assistance with animal care. This research was supported by USDA, ARS CRIS Project 6612-480-062 and Trust Agreement 193-6612-911. NR 27 TC 9 Z9 11 U1 0 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD FEB 25 PY 2015 VL 33 IS 9 BP 1197 EP 1205 DI 10.1016/j.vaccine.2014.12.028 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA CC2QO UT WOS:000350189900015 PM 25613723 ER PT J AU Zeller, WE Schatz, PF AF Zeller, Wayne E. Schatz, Paul F. TI Synthesis of monomethyl 5,5 '-dehydrodiferulic acid SO TETRAHEDRON LETTERS LA English DT Article DE Dehydrodiferulic acid; Hydroxycinnamate; Internal standard; Ferulate ID CEREAL DIETARY FIBER; MAIZE BRAN; FERULIC ACID; ENZYMATIC DEGRADATION; PHENOLIC DIMERS; CROSS-LINKING; CELL-WALLS; IDENTIFICATION; DEGRADABILITY; DEHYDROTRIMER AB Synthesis of the internal standard reference compound monomethyl 5,5'-dehydrodiferulic acid is described. The synthetic scheme relies on a selective monomethylation of the known compound 5,5'-dehydrodivanillin followed by elaboration into the dehydrodiferulic framework through a dual Horner-Emmons-Wadsworth reaction with methyl diethylphosphonoacetate. Saponification of the ester functionalities provided the target monomethyl 5,5'-dehydrodiferulic acid in 49% overall yield from 5,5'-dehydrodivanillin. Published by Elsevier Ltd. C1 [Zeller, Wayne E.; Schatz, Paul F.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Zeller, WE (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. EM Wayne.Zeller@ars.usda.gov; schatz@chem.wisc.edu FU NSF [CHE9974839] FX The authors would like to thank Jane Marita for purity assessment of compound la, providing the GC-MS traces and mass spectrum used in Figure 2, and for assistance in preparation of the Supporting material. Purchase of the Waters LCT (R) instrument in 2000 was partially funded by NSF Award #CHE9974839 to the Department of Chemistry, University of Wisconsin-Madison. Mention of trade names or commercial products in this Letter is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 26 TC 1 Z9 1 U1 2 U2 17 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0040-4039 J9 TETRAHEDRON LETT JI Tetrahedron Lett. PD FEB 25 PY 2015 VL 56 IS 9 BP 1076 EP 1079 DI 10.1016/j.tetlet.2015.01.002 PG 4 WC Chemistry, Organic SC Chemistry GA CC1FY UT WOS:000350087800006 ER PT J AU Leite, FL Reinhardt, TA Bannantine, JP Stabel, JR AF Leite, Fernando L. Reinhardt, Timothy A. Bannantine, John P. Stabel, Judith R. TI Envelope protein complexes of Mycobacterium avium subsp paratuberculosis and their antigenicity SO VETERINARY MICROBIOLOGY LA English DT Article DE Protein complexes; Mycobacterium avium subsp.; paratuberculosis; Antigens ID TANDEM MASS-SPECTROMETRY; JOHNES-DISEASE; GLUTAMINE-SYNTHETASE; STATISTICAL-MODEL; TUBERCULOSIS; IDENTIFICATION; MEMBRANE; ELECTROPHORESIS; PATHOGENESIS; SEQUENCE AB Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic enteric disease of ruminant animals. In the present study, blue native PAGE electrophoresis and 2D SDS-PAGE were used to separate MAP envelope protein complexes, followed by mass spectrometry (MS) to identify individual proteins within the complexes. Identity of individual proteins within complexes was further confirmed by MS upon excision of spots from 2D SDS-PAGE gels. Among the seven putative membrane complexes observed, major membrane protein (MAP2121c), a key MAP antigen involved in invasion of epithelial cells, was found to form a complex with cysteine desulfurase (MAP2120c). Other complexes found included those involved in energy metabolism (succinate dehydrogenase complex) as well as a complex formed by Cfp29, a characterized T cell antigen of Mycobacterium tuberculosis. To determine antigenicity of proteins, Western blot was performed on replicate 2D SDS-PAGE gels with sera from noninfected control cows (n = 9) and naturally infected cows in the subclinical (n = 10) and clinical (n = 13) stages of infection. Clinical animals recognized MAP2121c in greater proportion than subclinical and control cows, whereas cysteine desulfurase recognition was not differentiated by infection status. To further characterize antigenicity, recombinant proteins were expressed for 10 of the proteins identified and evaluated in an interferon-gamma (IFN-gamma) release assay as well as immunoblots. This study reveals the presence of protein complexes in the cell envelope of MAP, suggesting protein interactions in the envelope of this pathogen. Furthermore the identification of antigenic proteins with potential as diagnostic targets was characterized. Published by Elsevier B.V. C1 [Leite, Fernando L.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. [Reinhardt, Timothy A.; Bannantine, John P.; Stabel, Judith R.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Stabel, JR (reprint author), USDA ARS, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM fernando.leivasleite@gmail.com; tim.reinhardt@ars.usda.gov; john.bannantine@ars.usda.gov; judy.stabel@ars.usda.gov RI Reinhardt, Timothy/A-7536-2009; OI Reinhardt, Timothy/0000-0001-5552-2509; Bannantine, John/0000-0002-5692-7898 FU USDA-Agricultural Research Service FX The authors wish to thank Adrienne Staple, Janis Hansen, Margaret Walker, and Livia Eslabao for their technical help with this study. This work was funded by the USDA-Agricultural Research Service. NR 36 TC 2 Z9 3 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD FEB 25 PY 2015 VL 175 IS 2-4 BP 275 EP 285 DI 10.1016/j.vetmic.2014.11.009 PG 11 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CA5MI UT WOS:000348952100013 PM 25500374 ER PT J AU Cunha, CW Knowles, DP Taus, NS O'Toole, D Nicola, AV Aguilar, HC Li, H AF Cunha, Cristina W. Knowles, Donald P. Taus, Naomi S. O'Toole, Donal Nicola, Anthony V. Aguilar, Hector C. Li, Hong TI Antibodies to ovine herpesvirus 2 glycoproteins decrease virus infectivity and prevent malignant catarrhal fever in rabbits SO VETERINARY MICROBIOLOGY LA English DT Article DE OvHV-2; MCF; Antibody blocking; gB; gH/gL; Herpesvirus ID SHEEP NASAL SECRETIONS; MONOCLONAL-ANTIBODIES; NEUTRALIZATION; CATTLE; BISON; ENTRY; OUTBREAK; IMMUNITY; FUSION; TARGET AB Ovine herpesvirus-2 (OvHV-2) is the etiological agent of sheep-associated malignant catarrhal fever (SA-MCF), a fatal lymphoproliferative disease of many species in the order Artiodactyla. Development of a vaccine is critical to prevent mortality. Because OvHV-2 has not been cultured in vitro, SA-MCF research is hindered by the lack of in vitro tools to study viral constituents and specific host immune responses. As an alternative, in this study the neutralizing activity of antibodies against OvHV-2 glycoproteins gB and gH/gL was evaluated in vivo using rabbits. OvHV-2-specific antibodies were developed in rabbits by immunization using biolistic delivery of plasmids expressing the genes of interest. A lethal dose of OvHV-2 was incubated with the antisera and then nebulized into rabbits. Virus neutralization was assessed by measuring infection parameters associated with the virus infectious dose. Anti-gB or anti-gH/gL antibodies alone blocked infection in five out of six rabbits (83%), while a combination of anti-gB and anti-gH/gL antibodies protected all six rabbits (100%) from infection. These results indicate that antibodies to OvHV-2 gB and gH/gL are capable of neutralizing virions, and consequently, reduce virus infectivity and prevent SA-MCF in rabbits. Thus, OvHV-2 gB and gH/gL are suitable targets to be tested in a SA-MCF vaccine aimed at stimulating neutralizing antibody responses. (C) 2014 Elsevier B.V. All rights reserved. C1 [Cunha, Cristina W.; Knowles, Donald P.; Taus, Naomi S.; Nicola, Anthony V.; Li, Hong] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Knowles, Donald P.; Taus, Naomi S.; Li, Hong] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [O'Toole, Donal] Univ Wyoming, Wyoming State Vet Lab, Laramie, WY 82070 USA. [Aguilar, Hector C.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. RP Cunha, CW (reprint author), Washington State Univ, 3003 ADBF, Pullman, WA 99164 USA. EM cwcunha@vetmed.wsu.edu FU USDA-ARS [CWU 5348-32000-024-00D]; WSU New Faculty Seed Grant; NIH [A1096103, AI109022] FX This work was supported by USDA-ARS CWU 5348-32000-024-00D and WSU New Faculty Seed Grant to CWC. AVN is supported by NIH grant A1096103. HCA is supported by NIH grant AI109022. The authors thank Jana Marquard, Shirley Elias, Janice Keller and Nicholas Durfee for excellent technical assistance. NR 29 TC 2 Z9 2 U1 1 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD FEB 25 PY 2015 VL 175 IS 2-4 BP 349 EP 355 DI 10.1016/j.vetmic.2014.11.026 PG 7 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA CA5MI UT WOS:000348952100022 PM 25542288 ER PT J AU Postnikova, OA Hult, M Shao, J Skantar, A Nemchinov, LG AF Postnikova, Olga A. Hult, Maria Shao, Jonathan Skantar, Andrea Nemchinov, Lev G. TI Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita SO PLOS ONE LA English DT Article ID PLANTS; GENE; PROTEINS; SEQUENCE; REVEALS; DEFENSE; CULTURE; TOOL AB Nematodes are one of the major limiting factors in alfalfa production. Root-knot nematodes (RKN, Meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. As of today, no studies have been published on global gene expression profiling in alfalfa infected with RKN or any other plant parasitic nematode. Very little information is available about molecular mechanisms that contribute to pathogenesis and defense responses in alfalfa against these pests and specifically against RKN. In this work, we performed root transcriptome analysis of resistant (cv. Moapa 69) and susceptible (cv. Lahontan) alfalfa cultivars infected with RKN Meloidogyne incognita, widespread root-knot nematode species and a major pest worldwide. A total of 1,701,622,580 pair-end reads were generated on an Illumina Hi-Seq 2000 platform from the roots of both cultivars and assembled into 45,595 and 47,590 transcripts in cvs Moapa 69 and Lahontan, respectively. Bioinformatic analysis revealed a number of common and unique genes that were differentially expressed in susceptible and resistant lines as a result of nematode infection. Although the susceptible cultivar showed a more pronounced defense response to the infection, feeding sites were successfully established in its roots. Characteristically, basal gene expression levels under normal conditions differed between the two cultivars as well, which may confer advantage to one of the genotypes toward resistance to nematodes. Differentially expressed genes were subsequently assigned to known Gene Ontology categories to predict their functional roles and associated biological processes. Real-time PCR validated expression changes in genes arbitrarily selected for experimental confirmation. Candidate genes that contribute to protection against M. incognita in alfalfa were proposed and alfalfa-nematode interactions with respect to resistance are discussed. C1 [Postnikova, Olga A.; Shao, Jonathan; Nemchinov, Lev G.] USDA ARS, Beltsville Agr Res Ctr, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Hult, Maria; Skantar, Andrea] USDA ARS, Beltsville Agr Res Ctr, Nematol Lab, Beltsville, MD 20705 USA. RP Nemchinov, LG (reprint author), USDA ARS, Beltsville Agr Res Ctr, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM Lev.Nemchinov@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This work was supported by the United States Department of Agriculture, Agricultural Research Service. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 3 Z9 5 U1 2 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 24 PY 2015 VL 10 IS 2 AR e0118269 DI 10.1371/journal.pone.0118269 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC9IY UT WOS:000350683900051 PM 25710378 ER PT J AU Judd, TM Teal, PEA Hernandez, EJ Choudhury, T Hunt, JH AF Judd, Timothy M. Teal, Peter E. A. Hernandez, Edgar Javier Choudhury, Talbia Hunt, James H. TI Quantitative Differences in Nourishment Affect Caste-Related Physiology and Development in the Paper Wasp Polistes metricus SO PLOS ONE LA English DT Article ID FUSCATUS HYMENOPTERA-VESPIDAE; SOCIAL WASP; JUVENILE-HORMONE; GENE-EXPRESSION; MISCHOCYTTARUS-MASTIGOPHORUS; EVOLUTIONARY IMPLICATIONS; EUSOCIAL WASPS; APIS-MELLIFERA; COLD-HARDINESS; PREY QUANTITY AB The distinction between worker and reproductive castes of social insects is receiving increased attention from a developmental rather than adaptive perspective. In the wasp genus Polistes, colonies are founded by one or more females, and the female offspring that emerge in that colony are either non-reproducing workers or future reproductives of the following generation (gynes). A growing number of studies now indicate that workers emerge with activated reproductive physiology, whereas the future reproductive gynes do not. Low nourishment levels for larvae during the worker-rearing phase of the colony cycle and higher nourishment levels for larvae when gynes are reared are now strongly suspected of playing a major role in this difference. Here, we present the results of a laboratory rearing experiment in which Polistes metricus single foundresses were held in environmental conditions with a higher level of control than in any previously published study, and the amount of protein nourishment made available to feed larvae was the only input variable. Three experimental feeding treatments were tested: restricted, unrestricted, and hand-supplemented. Analysis of multiple response variables shows that wasps reared on restricted protein nourishment, which would be the case for wasps reared in field conditions that subsequently become workers, tend toward trait values that characterize active reproductive physiology. Wasps reared on unrestricted and hand-supplemented protein, which replicates higher feeding levels for larvae in field conditions that subsequently become gynes, tend toward trait values that characterize inactive reproductive physiology. Although the experiment was not designed to test for worker behavior per se, our results further implicate activated reproductive physiology as a developmental response to low larval nourishment as a fundamental aspect of worker behavior in Polistes. C1 [Judd, Timothy M.] SE Missouri State Univ, Dept Biol, Cape Girardeau, MO 63701 USA. [Teal, Peter E. A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32604 USA. [Hernandez, Edgar Javier] Univ Missouri, Dept Biol, St Louis, MO 63121 USA. [Choudhury, Talbia; Hunt, James H.] N Carolina State Univ, Dept Biol Sci, Raleigh, NC 27695 USA. [Hunt, James H.] N Carolina State Univ, WM Keck Ctr Behav Biol, Dept Entomol, Raleigh, NC 27695 USA. RP Judd, TM (reprint author), SE Missouri State Univ, Dept Biol, Cape Girardeau, MO 63701 USA. EM tjudd@semo.edu NR 81 TC 5 Z9 5 U1 4 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 23 PY 2015 VL 10 IS 2 AR e0116199 DI 10.1371/journal.pone.0116199 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC9BI UT WOS:000350662100028 PM 25706417 ER PT J AU DeVault, TL Blackwell, BF Seamans, TW Lima, SL Fernandez-Juricic, E AF DeVault, Travis L. Blackwell, Bradley F. Seamans, Thomas W. Lima, Steven L. Fernandez-Juricic, Esteban TI Speed kills: ineffective avian escape responses to oncoming vehicles SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE animal-vehicle collisions; antipredator behaviour; disturbance; escape response; flight initiation distance; video playback ID FLIGHT-INITIATION DISTANCE; AVOIDANCE-BEHAVIOR; ANIMAL BEHAVIOR; RISK-ASSESSMENT; BIRD STRIKES; VIDEO IMAGES; DISTURBANCE; DECISIONS; STIMULI; PREDATION AB Animal-vehicle collisions cause high levels of vertebrate mortality worldwide, and what goes wrong when animals fail to escape and ultimately collide with vehicles is not well understood. We investigated alert and escape behaviours of captive brown-headed cowbirds (Molothrus ater) in response to virtual vehicle approaches of different sizes and at speeds ranging from 60 to 360 km h(-1). Alert and flight initiation distances remained similar across vehicle speeds, and accordingly, alert and flight initiation times decreased at higher vehicle speeds. Thus, avoidance behaviours in cowbirds appeared to be based on distance rather than time available for escape, particularly at 60-150 km h(-1); however, at higher speeds (more than or equal to 180 km h(-1)) no trend in response behaviour was discernible. As vehicle speed increased, cowbirds did not have enough time to assess the approaching vehicle, and cowbirds generally did not initiate flight with enough time to avoid collision when vehicle speed exceeded 120 km h-1. Although potentially effective for evading predators, the decision-making process used by cowbirds in our study appears maladaptive in the context of avoiding fast-moving vehicles. Our methodological approach and findings provide a framework to assess how novel management strategies could affect escape rules, and the sensory and cognitive abilities animals use to avoid vehicle collisions. C1 [DeVault, Travis L.; Blackwell, Bradley F.; Seamans, Thomas W.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Lima, Steven L.] Indiana State Univ, Dept Biol, Terre Haute, IN 47809 USA. [Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. RP DeVault, TL (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. EM travis.l.devault@aphis.usda.gov FU US Department of Agriculture; Federal Aviation Administration FX The US Department of Agriculture and Federal Aviation Administration funded this research. NR 50 TC 7 Z9 7 U1 3 U2 37 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD FEB 22 PY 2015 VL 282 IS 1801 AR 20142188 DI 10.1098/rspb.2014.2188 PG 8 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA CC1BU UT WOS:000350077000017 PM 25567648 ER PT J AU Li, GQ Wang, Y Chen, MS Edae, E Poland, J Akhunov, E Chao, SM Bai, GH Carver, BF Yan, LL AF Li, Genqiao Wang, Ying Chen, Ming-Shun Edae, Erena Poland, Jesse Akhunov, Edward Chao, Shiaoman Bai, Guihua Carver, Brett F. Yan, Liuling TI Precisely mapping a major gene conferring resistance to Hessian fly in bread wheat using genotyping-by-sequencing SO BMC GENOMICS LA English DT Article DE Hessian fly resistance; Genotyping-by-sequencing (GBS); Insect resistance pathway; Quantitative trait loci (QTL); Wheat ID HEXAPLOID WHEAT; ASSOCIATION; MARKERS; IDENTIFICATION; CECIDOMYIIDAE; POPULATIONS; VIRULENCE; OKLAHOMA; DIPTERA; LINES AB Background: One of the reasons hard red winter wheat cultivar 'Duster' (PI 644016) is widely grown in the southern Great Plains is that it confers a consistently high level of resistance to biotype GP of Hessian fly (Hf). However, little is known about the genetic mechanism underlying Hf resistance in Duster. This study aimed to unravel complex structures of the Hf region on chromosome 1AS in wheat by using genotyping-by-sequencing (GBS) markers and single nucleotide polymorphism (SNP) markers. Results: Doubled haploid (DH) lines generated from a cross between two winter wheat cultivars, 'Duster' and 'Billings', were used to identify genes in Duster responsible for effective and consistent resistance to Hf. Segregation in reaction of the 282 DH lines to Hf biotype GP fit a one-gene model. The DH population was genotyped using 2,358 markers developed using the GBS approach. A major QTL, explaining 88% of the total phenotypic variation, was mapped to a chromosome region that spanned 178 cM and contained 205 GBSmarkers plus 1 SSR marker and 1 gene marker, with 0.86 cM per marker in genetic distance. The analyses of GBS marker sequences and further mapping of SSR and gene markers enabled location of the QTL-containing linkage group on the short arm of chromosome 1A. Comparative mapping of the common markers for the gene for QHf. osu-1A(d) in Duster and the Hf-resistance gene for QHf.osu-1A(74) in cultivar '2174' showed that the two Hf resistance genes are located on the same chromosome arm 1AS, only 11.2 cM apart in genetic distance. The gene at QHf. osu-1A(d) in Duster has been delimited within a 2.7 cM region. Conclusion: Two distinct resistance genes exist on the short arm of chromosome 1A as found in the two hard red winter cultivars, 2174 and Duster. Whereas the Hf resistance gene in 2174 is likely allelic to one or more of the previously mapped resistance genes (H9, H10, H11, H16, or H17) in wheat, the gene in Duster is novel and confers a more consistent phenotype than 2174 in response to biotype GP infestation in controlled-environment assays. C1 [Li, Genqiao; Wang, Ying; Carver, Brett F.; Yan, Liuling] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Chen, Ming-Shun; Edae, Erena; Bai, Guihua] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Poland, Jesse; Akhunov, Edward] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Chao, Shiaoman] ARS, USDA, Cereal Crops Res Unit, Fargo, ND 58102 USA. RP Yan, LL (reprint author), Oklahoma State Univ, Dept Plant & Soil Sci, 368 AG Hall, Stillwater, OK 74078 USA. EM liuling.yan@okstate.edu OI Poland, Jesse/0000-0002-7856-1399 FU USDA-NIFA T-CAP [2011-68002-30029]; Oklahoma Center of Advanced Science and Technology (OCAST); Oklahoma Wheat Research Foundation FX This work was supported by USDA-NIFA T-CAP grant No. 2011-68002-30029, the Oklahoma Center of Advanced Science and Technology (OCAST), and the Oklahoma Wheat Research Foundation. NR 31 TC 5 Z9 5 U1 4 U2 31 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 21 PY 2015 VL 16 AR 108 DI 10.1186/s12864-015-1297-7 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CC2HG UT WOS:000350165300001 PM 25765046 ER PT J AU Schumm, P Scoglio, C Zhang, Q Balcan, D AF Schumm, Phillip Scoglio, Caterina Zhang, Qian Balcan, Duygu TI Global epidemic invasion thresholds in directed cattle subpopulation networks having source, sink, and transit nodes SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article DE Simulation; Susceptible; Infected; Recovered ID RECURRENT MOBILITY PATTERNS; POTENTIAL DISEASE SPREAD; STRUCTURED POPULATIONS; INFECTIOUS-DISEASES; PANDEMIC INFLUENZA; ANIMAL MOVEMENTS; TRADE PATTERNS; GREAT-BRITAIN; TRANSPORTATION NETWORK; SPATIAL HETEROGENEITY AB Through the characterization of a metapopulation cattle disease model on a directed network having source, transit, and sink nodes, we derive two global epidemic invasion thresholds. The first threshold defines the conditions necessary for an epidemic to successfully spread at the global scale. The second threshold defines the criteria that permit an epidemic to move out of the giant strongly connected component and to invade the populations of the sink nodes. As each sink node represents a final waypoint for cattle before slaughter, the existence of an epidemic among the sink nodes is a serious threat to food security. We find that the relationship between these two thresholds depends on the relative proportions of transit and sink nodes in the system and the distributions of the in-degrees of both node types. These analytic results are verified through numerical realizations of the metapopulation cattle model. Published by Elsevier Ltd. C1 [Schumm, Phillip] USDA ARS, Ctr Grain & Anim Hlth Res, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. [Scoglio, Caterina] Kansas State Univ, Dept Elect & Comp Engn, Epictr, Manhattan, KS 66506 USA. [Zhang, Qian] Northeastern Univ, Coll Comp & Informat Sci, Lab Modeling Biol & Sociotech Syst, Boston, MA USA. [Balcan, Duygu] Istanbul Tech Univ, Dept Phys, Fac Sci & Letters, TR-34469 Istanbul, Turkey. RP Schumm, P (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM phillip.schumm@ars.usda.gov FU NSF [CNS-1006860, EPS-1006860, EPS-0919443]; National Agricultural Biosecurity Center at Kansas State University; US Department of Agriculture, the Agricultural Research Service [58-5430-1-0356] FX We would like to dedicate this paper to Dr. Duygu Balcan for her invaluable friendship and contributions to this work. Dr. Balcan has recently passed away in an unfortunate accident in August, 2013. We wish to convey our deepest condolences to all the lives she has touched. We would like to acknowledge Dr. Vespignani for the many insights and inputs from him and his research team regarding this work. A portion of this work was completed while PS was on sponsored visits to the Computational Epidemiology Laboratory at the Institute for Scientific Interchange, in Turin, Italy. The computing for this project was performed on the Beocat Research Cluster at Kansas State University, which is funded in part by NSF Grants CNS-1006860, EPS-1006860, and EPS-0919443. Partial funding for this project was provided by the National Agricultural Biosecurity Center at Kansas State University. This research was partially funded by US Department of Agriculture, the Agricultural Research Service with Specific Cooperative Agreement 58-5430-1-0356. NR 88 TC 1 Z9 1 U1 2 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 EI 1095-8541 J9 J THEOR BIOL JI J. Theor. Biol. PD FEB 21 PY 2015 VL 367 BP 203 EP 221 DI 10.1016/j.jtbi.2014.12.007 PG 19 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA CB3DT UT WOS:000349509100017 PM 25524151 ER PT J AU Aravalli, RN Talbot, NC Steer, CJ AF Aravalli, Rajagopal N. Talbot, Neil C. Steer, Clifford J. TI Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing SO WORLD JOURNAL OF GASTROENTEROLOGY LA English DT Article DE Hepatocellular carcinoma; MYC; Stem cells; Gene expression; RNA sequencing ID HEPATOCELLULAR-CARCINOMA CELLS; SELENIUM-BINDING PROTEIN-1; C-MYC; COLORECTAL-CANCER; RNA-SEQ; INTEGRIN ALPHA-3; LUNG-CANCER; FAMILY; PROLIFERATION; PROGRESSION AB AIM: To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem cells. METHODS: In this study, we have used an immortal porcine liver stem cell line, PICM-19, to study the role of c-MYC in hepatocarcinogenesis. PICM-19 cells were converted into cancer cells (PICM-19-CSCs) by over-expressing human MYC. To identify MYC-driven differential gene expression, transcriptome sequencing was carried out by RNA sequencing, and genes identified by this method were validated using real-time PCR. In vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient mice. RESULTS: Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a single oncogene was sufficient to convert them into cancer cells (PICM-19-CSCs). By using comparative bioinformatics analyses, we have determined that > 1000 genes were differentially expressed between PICM-19 and PICM-19-CSCs. Gene ontology analysis further showed that the MYC-induced, altered gene expression was primarily associated with various cellular processes, such as metabolism, cell adhesion, growth and proliferation, cell cycle, inflammation and tumorigenesis. Interestingly, six genes expressed by PICM-19 cells (CDO1, C22orf39, DKK2, ENPEP, GPX6, SRPX2) were completely silenced after MYC-induction in PICM-19-CSCs, suggesting that the absence of these genes may be critical for inducing tumorigenesis. CONCLUSION: MYC-driven genes may serve as promising candidates for the development of hepatocellular carcinoma therapeutics that would not have deleterious effects on other cell types in the liver. C1 [Aravalli, Rajagopal N.] Univ Minnesota, Sch Med, Dept Radiol, Minneapolis, MN 55455 USA. [Talbot, Neil C.] USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Steer, Clifford J.] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA. [Steer, Clifford J.] Univ Minnesota, Dept Genet, Minneapolis, MN 55455 USA. [Steer, Clifford J.] Univ Minnesota, Dept Cell Biol & Dev, Minneapolis, MN 55455 USA. RP Aravalli, RN (reprint author), Univ Minnesota, Sch Med, Dept Radiol, MMC 292 Mayo,420 Delaware St SE, Minneapolis, MN 55455 USA. EM arava001@umn.edu NR 94 TC 3 Z9 3 U1 1 U2 9 PU BAISHIDENG PUBLISHING GROUP INC PI PLEASANTON PA 8226 REGENCY DR, PLEASANTON, CA 94588 USA SN 1007-9327 EI 2219-2840 J9 WORLD J GASTROENTERO JI World J. Gastroenterol. PD FEB 21 PY 2015 VL 21 IS 7 BP 2011 EP 2029 DI 10.3748/wjg.v21.i7.2011 PG 19 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA CB5KY UT WOS:000349667100002 PM 25717234 ER PT J AU Jauregui, E Pacheco, AM Soltero, EG O'Connor, TM Castro, CM Estabrooks, PA McNeill, LH Lee, RE AF Jauregui, Edtna Pacheco, Ann M. Soltero, Erica G. O'Connor, Teresia M. Castro, Cynthia M. Estabrooks, Paul A. McNeill, Lorna H. Lee, Rebecca E. TI Using the RE-AIM framework to evaluate physical activity public health programs in Mexico SO BMC PUBLIC HEALTH LA English DT Article ID NATIONAL-HEALTH; DISEASE RISK; OBESITY; IMPACT; INTERVENTIONS; PREVALENCE; INACTIVITY; PROMOTION; COVERAGE; CHILDREN AB Background: Physical activity (PA) public health programming has been widely used in Mexico; however, few studies have documented individual and organizational factors that might be used to evaluate their public health impact. The RE-AIM framework is an evaluation tool that examines individual and organizational factors of public health programs. The purpose of this study was to use the RE-AIM framework to determine the degree to which PA programs in Mexico reported individual and organizational factors and to investigate whether reporting differed by the program's funding source. Methods: Public health programs promoting PA were systematically identified during 2008-2013 and had to have an active program website. Initial searches produced 23 possible programs with 12 meeting inclusion criteria. A coding sheet was developed to capture behavioral, outcome and RE-AIM indicators from program websites. Results: In addition to targeting PA, five (42%) programs also targeted dietary habits and the most commonly reported outcome was change in body composition (58%). Programs reported an average of 11.1 (similar to 3.9) RE-AIM indicator items (out of 27 total). On average, 45% reported reach indicators, 34% reported efficacy/ effectiveness indicators, 60% reported adoption indicators, 40% reported implementation indicators, and 35% reported maintenance indicators. The proportion of RE-AIM indicators reported did not differ significantly for programs that were government supported (M = 10, SD = 3.1) and programs that were partially or wholly privately or corporately supported (M = 12.0, SD = 4.4). Conclusion: While reach and adoption of these programs were most commonly reported, there is a need for stronger evaluation of behavioral and health outcomes before the public health impact of these programs can be established. C1 [Jauregui, Edtna] Univ Guadalajara, Ctr Univ Ciencias Salud, Inst Ciencias Aplicadas Actividad Fis & Deporte, Guadalajara 44430, Jalisco, Mexico. [Jauregui, Edtna] Secretaria Salud, Dept Prevent Med, Guadalajara, Jalisco, Mexico. [Pacheco, Ann M.; Soltero, Erica G.] Univ Houston, Dept Hlth & Human Performance, Texas Obes Res Ctr, Houston, TX USA. [O'Connor, Teresia M.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Castro, Cynthia M.] Stanford Univ, Sch Med, Stanford Prevent Res Ctr, Stanford, CA 94305 USA. [Estabrooks, Paul A.] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA. [McNeill, Lorna H.] Univ Texas Houston, MD Anderson Canc Res Ctr, Houston, TX 77030 USA. [Lee, Rebecca E.] Arizona State Univ, Coll Nursing & Hlth Innovat, Phoenix, AZ 85004 USA. RP Lee, RE (reprint author), Arizona State Univ, Coll Nursing & Hlth Innovat, 550 N 3rd St, Phoenix, AZ 85004 USA. EM releephd@yahoo.com FU Multinational Collaboration to Increase Physical Activity in Hispanics [1R13CA162816] FX This work was completed as part of a funded project called Multinational Collaboration to Increase Physical Activity in Hispanics (1R13CA162816) by the Applying Collaboration in Translational Investigation for Valuable Activity Recommendations (ACTIVAR) group. We thank Juan Lopez y Taylor, Edtna Jauregui, Charles Layne, Daniel O'Connor, Cynthia Castro, Lucie Lvesque, Lorna McNeill and Teresia O'Connor who were involved in this Multinational Collaboration project and who provided support and feedback. NR 32 TC 3 Z9 3 U1 3 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD FEB 19 PY 2015 VL 15 AR 162 DI 10.1186/s12889-015-1474-2 PG 9 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CD3BI UT WOS:000350952400001 PM 25881249 ER PT J AU Kowalski, KP Bacon, C Bickford, W Braun, H Clay, K Leduc-Lapierre, M Lillard, E McCormick, MK Nelson, E Torres, M White, J Wilcox, DA AF Kowalski, Kurt P. Bacon, Charles Bickford, Wesley Braun, Heather Clay, Keith Leduc-Lapierre, Michele Lillard, Elizabeth McCormick, Melissa K. Nelson, Eric Torres, Monica White, James Wilcox, Douglas A. TI Advancing the science of microbial symbiosis to support invasive species management: a case study on Phragmites in the Great Lakes SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE symbiosis; Phragmites; invasive species management; fungi; bacteria; collaborative; endophyte; Great Lakes Region ID COMMON REED; FUNGAL ENDOPHYTES; GUT MICROBIOTA; AUSTRALIS POPULATIONS; AMMOPHILA-ARENARIA; RHIZOSPHERE MICROBIOME; HYPERICUM-PERFORATUM; OOMYCETE COMMUNITIES; BACTERIAL DIVERSITY; BIOMASS PRODUCTION AB A growing body of literature supports microbial symbiosis as a foundational principle for the competitive success of invasive plant species. Further exploration of the relationships between invasive species and their associated microbiomes, as well as the interactions with the microbiomes of native species, can lead to key new insights into invasive success and potentially new and effective control approaches. In this manuscript, we review microbial relationships with plants, outline steps necessary to develop invasive species control strategies that are based on those relationships, and use the invasive plant species Phragmites australis (common reed) as an example of how development of microbial-based control strategies can be enhanced using a collective impact approach. The proposed science agenda, developed by the Collaborative for Microbial Symbiosis and Phragmites Management, contains a foundation of sequential steps and mutually-reinforcing tasks to guide the development of microbial-based control strategies for Phragmites and other invasive species. Just as the science of plant-microbial symbiosis can be transferred for use in other invasive species, so too can the model of collective impact be applied to other avenues of research and management. C1 [Kowalski, Kurt P.; Bickford, Wesley] US Geol Survey, Great Lakes Sci Ctr, Ann Arbor, MI 48105 USA. [Bacon, Charles] USDA ARS, Athens, GA 30613 USA. [Braun, Heather; Leduc-Lapierre, Michele; Lillard, Elizabeth] Great Lakes Commiss, Ann Arbor, MI USA. [Clay, Keith] Indiana Univ, Dept Biol, Bloomington, IN USA. [McCormick, Melissa K.] Smithsonian Environm Res Ctr, Edgewater, MD 21037 USA. [Nelson, Eric] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Torres, Monica; White, James] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA. [Wilcox, Douglas A.] SUNY Coll Brockport, Coll Brockport, Dept Environm Sci & Biol, Brockport, NY 14420 USA. RP Kowalski, KP (reprint author), US Geol Survey, Great Lakes Sci Ctr, 1451 Green Rd, Ann Arbor, MI 48105 USA. EM kkowalski@usgs.gov OI Kowalski, Kurt/0000-0002-8424-4701 FU U.S. Geological Survey; NOAA Maryland SeaGrant; Great Lakes Restoration Initiative allowed the research team FX Financial support from the U.S. Geological Survey, NOAA Maryland SeaGrant, and the Great Lakes Restoration Initiative allowed the research team to complete this project. We thank Katherine Hollins and other staff at the Great Lakes Commission for scientific and administrative contributions. We thank Aubrey Scott for background materials and formatting assistance. We thank Ellen Crocker for her early contributions to the process. Finally, we thank the known and anonymous reviewers for their constructive comments. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. This article is Contribution 1912 of the USGS Great Lakes Science Center. NR 159 TC 8 Z9 8 U1 8 U2 53 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 19 PY 2015 VL 6 DI 10.3389/fmicb.2015.00095 PG 14 WC Microbiology SC Microbiology GA CC4GC UT WOS:000350309100001 ER PT J AU Rugman-Jones, PF Seybold, SJ Graves, AD Stouthamer, R AF Rugman-Jones, Paul F. Seybold, Steven J. Graves, Andrew D. Stouthamer, Richard TI Phylogeography of the Walnut Twig Beetle, Pityophthorus juglandis, the Vector of Thousand Cankers Disease in North American Walnut Trees SO PLOS ONE LA English DT Article ID MITOCHONDRIAL-DNA; THYSANOPTERA THRIPIDAE; POPULATION-GENETICS; MAXIMUM-LIKELIHOOD; GEOSMITHIA-MORBIDA; FUNGUS SYMBIOSES; BARK; COLEOPTERA; INSECTS; IDENTIFICATION AB Thousand cankers disease (TCD) of walnut trees (Juglans spp.) results from aggressive feeding in the phloem by the walnut twig beetle (WTB), Pityophthorus juglandis, accompanied by inoculation of its galleries with a pathogenic fungus, Geosmithia morbida. In 1960, WTB was only known from four U.S. counties (in Arizona, California, and New Mexico), but the species has now (2014) invaded over 115 counties, representing much of the western USA, and at least six states in the eastern USA. The eastern expansion places TCD in direct proximity to highly valuable (> $500 billion) native timber stands of eastern black walnut, Juglans nigra. Using mitochondrial DNA sequences, from nearly 1100 individuals, we examined variation among 77 samples of WTB populations across its extended range in the USA, revealing high levels of polymorphism and evidence of two divergent lineages. The highest level of genetic diversity for the different lineages was found in the neighboring Madrean Sky Island and Western New Mexico regions, respectively. Despite their proximity, there was little evidence of mixing between these regions, with only a single migrant detected among 179 beetles tested. Indeed, geographic overlap of the two lineages was only common in parts of Colorado and Utah. Just two haplotypes, from the same lineage, predominated over the vast majority of the recently expanded range. Tests for Wolbachia proved negative suggesting it plays no role in "driving" the spread of particular haplotypes, or in maintaining deep levels of intraspecific divergence in WTB. Genotyping of ribosomal RNA corroborated the mitochondrial lineages, but also revealed evidence of hybridization between them. Hybridization was particularly prevalent in the sympatric areas, also apparent in all invaded areas, but absent from the most haplotype-rich area of each mitochondrial lineage. Hypotheses about the specific status of WTB, its recent expansion, and potential evolutionary origins of TCD are discussed. C1 [Rugman-Jones, Paul F.; Stouthamer, Richard] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Seybold, Steven J.] USDA, Forest Serv, Pacific Southwest Res Stn, Davis, CA USA. [Graves, Andrew D.] USDA, Forest Serv, Forest Hlth Protect, Albuquerque, NM USA. RP Rugman-Jones, PF (reprint author), Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. EM paul.rugman-jones@ucr.edu FU USDA Forest Service; Pacific Southwest Research Station; USDA Forest Service, Washington Office Forest Health Protection; USDA APHIS CPHST [10-CA-11272172-058] FX Funding for this work was provided by the USDA Forest Service, Pacific Southwest Research Station, and by grants from the USDA Forest Service, Washington Office Forest Health Protection and USDA APHIS CPHST administered by Mary Louise Flint through cooperative agreement #10-CA-11272172-058 between the USDA FS PSW Station and the UC-Riverside Department of Entomology. The authors are especially grateful to Bruce Moltzan for facilitating the funding from the USDA FS and to David Lance for facilitating the funding from USDA APHIS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 7 Z9 7 U1 7 U2 37 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 19 PY 2015 VL 10 IS 2 AR e0118264 DI 10.1371/journal.pone.0118264 PG 28 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC0XE UT WOS:000350062300046 PM 25695760 ER PT J AU Kundaje, A Meuleman, W Ernst, J Bilenky, M Yen, A Heravi-Moussavi, A Kheradpour, P Zhang, Z Wang, J Ziller, MJ Amin, V Whitaker, JW Schultz, MD Ward, LD Sarkar, A Quon, G Sandstrom, RS Eaton, ML Wu, YC Pfenning, AR Wang, X Claussnitzer, M Liu, Y Coarfa, C Harris, RA Shoresh, N Epstein, CB Gjoneska, E Leung, D Xie, W Hawkins, RD Lister, R Hong, C Gascard, P Mungall, AJ Moore, R Chuah, E Tam, A Canfield, TK Hansen, RS Kaul, R Sabo, PJ Bansal, MS Carles, A Dixon, JR Farh, KH Feizi, S Karlic, R Kim, AR Kulkarni, A Li, D Lowdon, R Elliott, G Mercer, TR Neph, SJ Onuchic, V Polak, P Rajagopal, N Ray, P Sallari, RC Siebenthall, KT Sinnott-Armstrong, NA Stevens, M Thurman, RE Wu, J Zhang, B Zhou, X Beaudet, AE Boyer, LA De Jager, PL Farnham, PJ Fisher, SJ Haussler, D Jones, SJM Li, W Marra, MA McManus, MT Sunyaev, S Thomson, JA Tlsty, TD Tsai, LH Wang, W Waterland, RA Zhang, MQ Chadwick, LH Bernstein, BE Costello, JF Ecker, JR Hirst, M Meissner, A Milosavljevic, A Ren, B Stamatoyannopoulos, JA Wang, T Kellis, M AF Kundaje, Anshul Meuleman, Wouter Ernst, Jason Bilenky, Misha Yen, Angela Heravi-Moussavi, Alireza Kheradpour, Pouya Zhang, Zhizhuo Wang, Jianrong Ziller, Michael J. Amin, Viren Whitaker, John W. Schultz, Matthew D. Ward, Lucas D. Sarkar, Abhishek Quon, Gerald Sandstrom, Richard S. Eaton, Matthew L. Wu, Yi-Chieh Pfenning, Andreas R. Wang, Xinchen Claussnitzer, Melina Liu, Yaping Coarfa, Cristian Harris, R. Alan Shoresh, Noam Epstein, Charles B. Gjoneska, Elizabeta Leung, Danny Xie, Wei Hawkins, R. David Lister, Ryan Hong, Chibo Gascard, Philippe Mungall, Andrew J. Moore, Richard Chuah, Eric Tam, Angela Canfield, Theresa K. Hansen, R. Scott Kaul, Rajinder Sabo, Peter J. Bansal, Mukul S. Carles, Annaick Dixon, Jesse R. Farh, Kai-How Feizi, Soheil Karlic, Rosa Kim, Ah-Ram Kulkarni, Ashwinikumar Li, Daofeng Lowdon, Rebecca Elliott, GiNell Mercer, Tim R. Neph, Shane J. Onuchic, Vitor Polak, Paz Rajagopal, Nisha Ray, Pradipta Sallari, Richard C. Siebenthall, Kyle T. Sinnott-Armstrong, Nicholas A. Stevens, Michael Thurman, Robert E. Wu, Jie Zhang, Bo Zhou, Xin Beaudet, Arthur E. Boyer, Laurie A. De Jager, Philip L. Farnham, Peggy J. Fisher, Susan J. Haussler, David Jones, Steven J. M. Li, Wei Marra, Marco A. McManus, Michael T. Sunyaev, Shamil Thomson, James A. Tlsty, Thea D. Tsai, Li-Huei Wang, Wei Waterland, Robert A. Zhang, Michael Q. Chadwick, Lisa H. Bernstein, Bradley E. Costello, Joseph F. Ecker, Joseph R. Hirst, Martin Meissner, Alexander Milosavljevic, Aleksandar Ren, Bing Stamatoyannopoulos, John A. Wang, Ting Kellis, Manolis CA Roadmap Epigenomics Consortium TI Integrative analysis of 111 reference human epigenomes SO NATURE LA English DT Article ID GENOME-WIDE ASSOCIATION; EMBRYONIC STEM-CELLS; DYNAMIC DNA METHYLATION; CHROMATIN-STATE; HISTONE MODIFICATIONS; RISK LOCI; TRANSCRIPTION FACTORS; ALZHEIMERS-DISEASE; REGULATORY REGIONS; MAMMALIAN GENOMES AB The reference human genome sequence set the stage for studies of genetic variation and its association with human disease, but epigenomic studies lack a similar reference. To address this need, the NIH Roadmap Epigenomics Consortium generated the largest collection so far of human epigenomes for primary cells and tissues. Here we describe the integrative analysis of 111 reference human epigenomes generated as part of the programme, profiled for histone modification patterns, DNA accessibility, DNA methylation and RNA expression. We establish global maps of regulatory elements, define regulatory modules of coordinated activity, and their likely activators and repressors. We show that disease- and trait-associated genetic variants are enriched in tissue-specific epigenomic marks, revealing biologically relevant cell types for diverse human traits, and providing a resource for interpreting the molecular basis of human disease. Our results demonstrate the central role of epigenomic information for understanding gene regulation, cellular differentiation and human disease. C1 [Kundaje, Anshul; Meuleman, Wouter; Ernst, Jason; Yen, Angela; Kheradpour, Pouya; Zhang, Zhizhuo; Wang, Jianrong; Ward, Lucas D.; Sarkar, Abhishek; Quon, Gerald; Eaton, Matthew L.; Wu, Yi-Chieh; Pfenning, Andreas R.; Wang, Xinchen; Claussnitzer, Melina; Liu, Yaping; Bansal, Mukul S.; Feizi, Soheil; Kim, Ah-Ram; Sallari, Richard C.; Sinnott-Armstrong, Nicholas A.; Kellis, Manolis] MIT, Comp Sci & Artificial Intelligence Lab, Cambridge, MA 02139 USA. [Kundaje, Anshul; Meuleman, Wouter; Ernst, Jason; Yen, Angela; Kheradpour, Pouya; Zhang, Zhizhuo; Wang, Jianrong; Ziller, Michael J.; Ward, Lucas D.; Sarkar, Abhishek; Quon, Gerald; Eaton, Matthew L.; Wu, Yi-Chieh; Pfenning, Andreas R.; Wang, Xinchen; Claussnitzer, Melina; Liu, Yaping; Shoresh, Noam; Epstein, Charles B.; Gjoneska, Elizabeta; Bansal, Mukul S.; Farh, Kai-How; Feizi, Soheil; Kim, Ah-Ram; Polak, Paz; Sallari, Richard C.; Sinnott-Armstrong, Nicholas A.; De Jager, Philip L.; Sunyaev, Shamil; Tsai, Li-Huei; Bernstein, Bradley E.; Meissner, Alexander; Kellis, Manolis] Broad Inst Harvard & MIT, Cambridge, MA 02142 USA. [Kundaje, Anshul] Stanford Univ, Dept Genet, Dept Comp Sci, Stanford, CA 94305 USA. [Ernst, Jason] Univ Calif Davis, Dept Biol Chem, Los Angeles, CA 90095 USA. [Bilenky, Misha; Heravi-Moussavi, Alireza; Mungall, Andrew J.; Moore, Richard; Chuah, Eric; Tam, Angela; Jones, Steven J. M.; Marra, Marco A.; Hirst, Martin] BC Canc Agcy, Canadas Michael Smith Genome Sci Ctr, Vancouver, BC V5Z 1L3, Canada. [Ziller, Michael J.; Meissner, Alexander] Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA. [Amin, Viren; Coarfa, Cristian; Harris, R. Alan; Onuchic, Vitor; Milosavljevic, Aleksandar] Baylor Coll Med, Epigenome Ctr, Houston, TX 77030 USA. [Whitaker, John W.; Leung, Danny; Xie, Wei; Hawkins, R. David; Dixon, Jesse R.; Rajagopal, Nisha; Wang, Wei; Ren, Bing] Univ Calif San Diego, Inst Genom Med, Dept Cellular & Mol Med, Dept Chem & Biochem, La Jolla, CA 92093 USA. [Schultz, Matthew D.; Lister, Ryan; Ecker, Joseph R.] Howard Hughes Med Inst, Genom Anal Lab, La Jolla, CA 92037 USA. [Schultz, Matthew D.; Lister, Ryan; Ecker, Joseph R.] Salk Inst Biol Studies, La Jolla, CA 92037 USA. [Sandstrom, Richard S.; Canfield, Theresa K.; Sabo, Peter J.; Neph, Shane J.; Siebenthall, Kyle T.; Thurman, Robert E.; Stamatoyannopoulos, John A.] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Wang, Xinchen; Boyer, Laurie A.] MIT, Dept Biol, Cambridge, MA 02142 USA. [Gjoneska, Elizabeta; Tsai, Li-Huei] MIT, Picower Inst Learning & Memory, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA. [Leung, Danny; Xie, Wei; Hawkins, R. David; Dixon, Jesse R.; Rajagopal, Nisha; Ren, Bing] Ludwig Inst Canc Res, La Jolla, CA 92093 USA. [Hong, Chibo; Costello, Joseph F.] Univ Calif San Francisco, Helen Diller Family Comprehens Canc Ctr, Dept Neurosurg, San Francisco, CA 94158 USA. [Gascard, Philippe; Tlsty, Thea D.] Univ Calif San Francisco, Dept Pathol, San Francisco, CA 94140 USA. [Hansen, R. Scott; Kaul, Rajinder] Univ Washington, Div Med Genet, Dept Med, Seattle, WA 98121 USA. [Bansal, Mukul S.] Univ Connecticut, Dept Comp Sci & Engn, Storrs, CT 06269 USA. [Carles, Annaick; Hirst, Martin] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V6T 1Z4, Canada. [Carles, Annaick; Hirst, Martin] Univ British Columbia, Ctr High Throughput Biol, Vancouver, BC V6T 1Z4, Canada. [Karlic, Rosa] Univ Zagreb, Div Biol, Dept Mol Biol, Bioinformat Grp, Zagreb 10000, Croatia. [Kulkarni, Ashwinikumar; Ray, Pradipta; Zhang, Michael Q.] Univ Texas Dallas, Dept Mol & Cell Biol, Ctr Syst Biol, NSERL, Dallas, TX 75080 USA. [Li, Daofeng; Lowdon, Rebecca; Elliott, GiNell; Stevens, Michael; Zhang, Bo; Zhou, Xin; Wang, Ting] Washington Univ, Dept Genet, Ctr Genome Sci & Syst Biol, St Louis, MO 63108 USA. [Mercer, Tim R.] Univ Queensland, Inst Mol Biosci, St Lucia, Qld 4072, Australia. [Polak, Paz; De Jager, Philip L.; Sunyaev, Shamil] Brigham & Womens Hosp, Boston, MA 02115 USA. [Wu, Jie] SUNY Stony Brook, Dept Appl Math & Stat, Stony Brook, NY 11794 USA. [Wu, Jie] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Beaudet, Arthur E.] Baylor Coll Med, Mol & Human Genet Dept, Houston, TX 77030 USA. [De Jager, Philip L.; Sunyaev, Shamil] Harvard Univ, Sch Med, Boston, MA 02115 USA. [Farnham, Peggy J.] Univ So Calif, Keck Sch Med, Dept Biochem, Los Angeles, CA 90089 USA. [Fisher, Susan J.] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Haussler, David] Univ Calif Santa Cruz, Ctr Biomol Sci & Engn, Santa Cruz, CA 95064 USA. [Jones, Steven J. M.] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada. [Jones, Steven J. M.; Marra, Marco A.] Univ British Columbia, Dept Med Genet, Vancouver, BC V6T 1Z4, Canada. [Li, Wei] Baylor Coll Med, Dan L Duncan Canc Ctr, Houston, TX 77030 USA. [McManus, Michael T.] Univ Calif San Francisco, Ctr Diabet, Dept Microbiol & Immunol, San Francisco, CA 94143 USA. [Thomson, James A.] Univ Wisconsin, Madison, WI 53715 USA. [Waterland, Robert A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Zhang, Michael Q.] Tsinghua Univ, Bioinformat Div, Ctr Synthet & Syst Biol, TNLIST, Beijing 100084, Peoples R China. [Chadwick, Lisa H.] NIEHS, Res Triangle Pk, NC 27709 USA. [Bernstein, Bradley E.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Bernstein, Bradley E.] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. [Thomson, James A.] Morgridge Inst Res, Madison, WI 53707 USA. [Stevens, Michael] Washington Univ, Dept Comp Sci & Engn, St Louis, MO 63130 USA. RP Kundaje, A (reprint author), MIT, Comp Sci & Artificial Intelligence Lab, 32 Vassar St, Cambridge, MA 02139 USA. EM manoli@mit.edu RI Mattick, John/I-7789-2012; Wang, Tao/E-5900-2015; Chen, Zhao/I-5625-2014; Lister, Ryan/B-5168-2012; Li, Daofeng/E-5386-2011; Ecker, Joseph/B-9144-2008; Hariharan, Manoj/N-2855-2015; Hirst, Martin/B-7684-2016; Marra, Marco/B-5987-2008; Jones, Steven/C-3621-2009; Zhang, Bo/A-6874-2011; OI Meuleman, Wouter/0000-0002-1196-5401; Pelizzola, Mattia/0000-0001-6672-9636; Ward, Lucas/0000-0002-8017-809X; Tsai, Linus/0000-0002-0134-6949; Mattick, John/0000-0002-7680-7527; Gu, Junchen/0000-0002-0928-7453; /0000-0002-3447-9801; Wang, Tao/0000-0002-1646-6863; Lister, Ryan/0000-0001-6637-7239; Li, Daofeng/0000-0001-7492-3703; Ecker, Joseph/0000-0001-5799-5895; Hariharan, Manoj/0000-0002-1006-5372; Zhang, Bo/0000-0003-2962-5314; Farnham, Peggy/0000-0003-4469-7914; Robinson, Joshua/0000-0002-2421-4535; Subramanian, Sai Lakshmi/0000-0003-1384-0722 FU NIH Common Fund as part of the NIH Roadmap Epigenomics Program [U01ES017155, U01ES017154, U01ES017166, U01ES017156, U01DA025956]; NHGRI [RC1HG005334, R01HG004037, R01HG004037-S1, RO1NS078839]; NIH [ES017166, F32HL110473, K99HL119617]; NSFC [91019016]; NBRPC [2012CB316503]; NSF CAREER [1254200]; [5R24HD000836]; [P30AG10161]; [R01AG15819]; [R01AG17917]; [U01AG46152] FX This work was supported by the NIH Common Fund as part of the NIH Roadmap Epigenomics Program through U01ES017155 (B.B. and A.M.), U01ES017154 (J.C. and M.M.), U01ES017166 (B.R.), U01ES017156 (J.S.), U01DA025956 (A.M. and A.B.), and by NHGRI through RC1HG005334, R01HG004037 and R01HG004037-S1 (M.K.), RO1NS078839 (L.-H.T.), and by NIH ES017166, NSFC 91019016 and NBRPC 2012CB316503 (M.Q.Z.). Sample procurement was supported by grants 5R24HD000836 (I.A.G.) for staged fetal tissues; P30AG10161, R01AG15819, R01AG17917 (D.A.B.) and U01AG46152 (P.L.D. and D.A.B.) for adult brain samples. This work was also supported by NIH fellowship grants F32HL110473 and K99HL119617 (S.L.), and NSF CAREER award 1254200 (J.E.). We acknowledge program leadership by members of the NIH Epigenomics Workgroup, especially J.S. Satterlee, F.L. Tyson, J. Rutter, K.A. McAllister, A. Haugen, C. Colvis (NCATS), J. Battey (NIDCD), L. Birnbaum (NIEHS) and N. Volkow (NIDA). We acknowledge feedback from our External Scientific Panel members M. Bartolomei, S. Baylin, S. Beck, A. Chakravarti, L. Jackson-Grusby, J. Lieb, S. Peckman, J. Quackenbush and S. Stice. NR 112 TC 305 Z9 307 U1 21 U2 162 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD FEB 19 PY 2015 VL 518 IS 7539 BP 317 EP 330 DI 10.1038/nature14248 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB3SA UT WOS:000349547400028 ER PT J AU Richman, NI Boehm, M Adams, SB Alvarez, F Bergey, EA Bunn, JJS Burnham, Q Cordeiro, J Coughran, J Crandall, KA Dawkins, KL DiStefano, RJ Doran, NE Edsman, L Eversole, AG Fureder, L Furse, JM Gherardi, F Hamr, P Holdich, DM Horwitz, P Johnston, K Jones, CM Jones, JPG Jones, RL Jones, TG Kawai, T Lawler, S Lopez-Mejia, M Miller, RM Pedraza-Lara, C Reynolds, JD Richardson, AMM Schultz, MB Schuster, GA Sibley, PJ Souty-Grosset, C Taylor, CA Thoma, RF Walls, J Walsh, TS Collen, B AF Richman, Nadia I. Boehm, Monika Adams, Susan B. Alvarez, Fernando Bergey, Elizabeth A. Bunn, John J. S. Burnham, Quinton Cordeiro, Jay Coughran, Jason Crandall, Keith A. Dawkins, Kathryn L. DiStefano, Robert J. Doran, Niall E. Edsman, Lennart Eversole, Arnold G. Fuereder, Leopold Furse, James M. Gherardi, Francesca Hamr, Premek Holdich, David M. Horwitz, Pierre Johnston, Kerrylyn Jones, Clive M. Jones, Julia P. G. Jones, Robert L. Jones, Thomas G. Kawai, Tadashi Lawler, Susan Lopez-Mejia, Marilu Miller, Rebecca M. Pedraza-Lara, Carlos Reynolds, Julian D. Richardson, Alastair M. M. Schultz, Mark B. Schuster, Guenter A. Sibley, Peter J. Souty-Grosset, Catherine Taylor, Christopher A. Thoma, Roger F. Walls, Jerry Walsh, Todd S. Collen, Ben TI Multiple drivers of decline in the global status of freshwater crayfish (Decapoda: Astacidea) SO PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE extinction risk; crayfish; IUCN Red List; threatened; freshwater biodiversity ID EXTINCTION RISK; CLIMATE-CHANGE; BIODIVERSITY CONSERVATION; EASTERN AUSTRALIA; SIGNAL CRAYFISH; UNITED-STATES; PARASTACIDAE; MANAGEMENT; PRIORITIES; BIOLOGY AB Rates of biodiversity loss are higher in freshwater ecosystems than in most terrestrial or marine ecosystems, making freshwater conservation a priority. However, prioritization methods are impeded by insufficient knowledge on the distribution and conservation status of freshwater taxa, particularly invertebrates. We evaluated the extinction risk of the world's 590 freshwater crayfish species using the IUCN Categories and Criteria and found 32% of all species are threatened with extinction. The level of extinction risk differed between families, with proportionally more threatened species in the Parastacidae and Astacidae than in the Cambaridae. Four described species were Extinct and 21% were assessed as Data Deficient. There was geographical variation in the dominant threats affecting the main centres of crayfish diversity. The majority of threatened US and Mexican species face threats associated with urban development, pollution, damming and water management. Conversely, the majority of Australian threatened species are affected by climate change, harvesting, agriculture and invasive species. Only a small proportion of crayfish are found within the boundaries of protected areas, suggesting that alternative means of long-term protection will be required. Our study highlights many of the significant challenges yet to come for freshwater biodiversity unless conservation planning shifts from a reactive to proactive approach. C1 [Richman, Nadia I.; Boehm, Monika] Zool Soc London, Inst Zool, London NW1 4RY, England. [Richman, Nadia I.; Jones, Julia P. G.] Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2UW, Gwynedd, Wales. [Adams, Susan B.] US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. [Alvarez, Fernando] Univ Nacl Autonoma Mexico, Inst Biol, Colecc Nacl Crustaceos, Mexico City 04510, DF, Mexico. [Bergey, Elizabeth A.] Univ Oklahoma, Oklahoma Biol Survey, Norman, OK 73019 USA. [Bergey, Elizabeth A.] Univ Oklahoma, Dept Biol, Norman, OK 73019 USA. [Bunn, John J. S.; Burnham, Quinton; Coughran, Jason; Horwitz, Pierre] Edith Cowan Univ, Sch Nat Sci, Joondalup, WA, Australia. [Cordeiro, Jay] Northeast Nat Hist & Supply, Middleboro, MA 02346 USA. [Coughran, Jason] Jagabar Environm, Duncraig, WA 6023, Australia. [Crandall, Keith A.] George Washington Univ, Computat Biol Inst, Ashburn, VA 20147 USA. [Crandall, Keith A.] Smithsonian Inst, Natl Museum Nat Hist, Dept Invertebrate Zool, Washington, DC 20013 USA. [Dawkins, Kathryn L.] Griffith Univ, Griffith Sch Environm, Australian Rivers Inst, Gold Coast Campus, Qld 4222, Australia. [DiStefano, Robert J.] Missouri Dept Conservat, Columbia, MO 65201 USA. [Doran, Niall E.] Univ Tasmania, Bookend Trust, Sandy Bay, Tas 7006, Australia. [Doran, Niall E.] Univ Tasmania, Sch Biol Sci, Sandy Bay, Tas 7006, Australia. [Edsman, Lennart] Swedish Univ Agr Sci, Dept Aquat Resources, Inst Freshwater Res, S-17893 Drottningholm, Sweden. [Eversole, Arnold G.] Clemson Univ, Sch Agr Forestry & Environm Sci, Clemson, SC 29634 USA. [Fuereder, Leopold] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. [Furse, James M.] Griffith Univ, Griffith Sch Environm, Gold Coast Campus, Qld 4222, Australia. [Furse, James M.] Griffith Univ, Environm Futures Res Inst, Gold Coast Campus, Qld 4222, Australia. [Gherardi, Francesca] Univ Florence, Dipartimento Biol, I-50125 Florence, Italy. [Hamr, Premek] Upper Canada Coll, Toronto, ON M4V 1W6, Canada. [Miller, Rebecca M.] Peak Ecol Ltd, Crayfish Survey, Bakewell DE45 1GT, Derby, England. [Holdich, David M.] Peak Ecol Ltd, Res, Bakewell DE45 1GT, Derby, England. [Johnston, Kerrylyn] Murdoch Univ, Murdoch, WA 6150, Australia. [Johnston, Kerrylyn] Murdoch Univ, Marine & Freshwater Res Lab, Murdoch, WA 6150, Australia. [Jones, Clive M.] James Cook Univ, Sch Marine & Trop Biol, Cairns, Qld 4870, Australia. [Jones, Robert L.] Museum Nat Sci, Mississippi Dept Wildlife Fisheries & Pk, Jackson, MS 39202 USA. [Jones, Thomas G.] Marshall Univ, Dept Integrated Sci & Technol, Huntington, WV 25755 USA. [Kawai, Tadashi] Wakkanai Fisheries Inst, Wakkanai, Hokkaido 0970001, Japan. [Lawler, Susan] La Trobe Univ, Dept Environm Management & Ecol, Wodonga, Vic 3690, Australia. [Lopez-Mejia, Marilu] Univ Quintana Roo, Evolutionary Biol & Populat Genet Lab, Unidad Acad Cozumel, Cozumel 77600, Q Roo, Mexico. [Miller, Rebecca M.] Global Ecosyst Management Programme, Int Union Conservat Nat, Cambridge CB3 0DL, England. [Pedraza-Lara, Carlos] Univ Nacl Autonoma Mexico, Fac Med, Mexico City 04510, DF, Mexico. [Pedraza-Lara, Carlos] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico. [Reynolds, Julian D.] Trinity Coll Dublin, Stillorgan, Dublin, Ireland. [Richardson, Alastair M. M.] Univ Tasmania, Sch Biol, Hobart, Tas 7001, Australia. [Schultz, Mark B.] Univ Melbourne, Dept Biochem & Mol Biol, Melbourne, Vic 3010, Australia. [Sibley, Peter J.] Environm Agcy, Wessex Area, East Quay TA6 4YS, Bridgwater, England. [Souty-Grosset, Catherine] Univ Poitiers, UMR CNRS 7267, Lab Ecol & Biol Interact, Equipe Ecol Evolut Symbiose, Poitiers, France. [Taylor, Christopher A.] Illinois Nat Hist Survey, Prairie Res Inst, Champaign, IL 61820 USA. [Thoma, Roger F.] Midwest Biodivers Inst, Hilliard, OH 43026 USA. [Walls, Jerry] Louisiana State Univ, Dept Biol Sci, Alexandria, LA 71302 USA. [Collen, Ben] UCL, Ctr Biodivers & Environm Res, London WC1E 6BT, England. RP Richman, NI (reprint author), Zool Soc London, Inst Zool, Regents Pk, London NW1 4RY, England. EM nadia.richman@ioz.ac.uk RI Jones, Clive/D-2991-2009; CSTFA, ResearcherID/P-1067-2014; Jones, Julia/A-3031-2011; Collen, Ben/F-2543-2016 OI Jones, Clive/0000-0002-6319-2068; Crandall, Keith/0000-0002-0836-3389; Dawkins, Kathryn/0000-0001-5092-2378; Schultz, Mark/0000-0002-7689-6531; Jones, Julia/0000-0002-5199-3335; Collen, Ben/0000-0003-2564-4243 FU Esmee Fairbairn Foundation; Rufford Foundation; Biodiversity Synthesis Center in Chicago FX The authors acknowledge the Esmee Fairbairn Foundation (N.I.R. and M.B.), the Rufford Foundation (B.C. and M.B.) and the Biodiversity Synthesis Center in Chicago who funded and hosted the workshop in which all North American species were assessed. For data compilation, we thank Alison Batchelor, Georgia Cryer, Ranmali De Silva, Ellie Dyer, Blythe Jopling, Gita Kasthala, Fiona Livingston, Maiko Lutz, Shane McGuinness, Harriet Milligan, Mala Ram, Jeremy Smith, Anne-Marie Soulsby, Kathryn Sullivan and Felix Whitton. We also acknowledge the vast network of data contributors who have been kind enough to review reports; Christopher Austin, Miklos Puky, Eva Kozubikova, William R. Elliott, John Foster, Holga Schulz, Ivana Maguire, Japo Jussila, Stephanie Peay, Peter Manko, Emmanuil Manos, Marina Paolucci, Martin Pugh, Naomi Wilson, Paul Bradley, Ralf Schulz, Boris Sket, Soren Berg, Adrian Hutchings, Angel Zaikov, Margaret Palmer, Daniel Hefti, Yoann Allanic, Markku Pursiainen, Liviu Miron, Lucian Parvulescu, Peter Manko, Roger Cammaerts, Valery Fedotov, Olga Mandryka, Pedro Gutierrez and Przemyslaw Smietana. We thank our two anonymous referees for helpful comments on an earlier version of this paper. NR 90 TC 22 Z9 23 U1 5 U2 150 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8436 EI 1471-2970 J9 PHILOS T R SOC B JI Philos. Trans. R. Soc. B-Biol. Sci. PD FEB 19 PY 2015 VL 370 IS 1662 AR 20140060 DI 10.1098/rstb.2014.0060 PG 11 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AY0UM UT WOS:000347312400017 PM 25561679 ER PT J AU Ribeiro, DN Nandula, VK Dayan, FE Rimando, AM Duke, SO Reddy, KN Shaw, DR AF Ribeiro, Daniela N. Nandula, Vijay K. Dayan, Franck E. Rimando, Agnes M. Duke, Stephen O. Reddy, Krishna N. Shaw, David R. TI Possible Glyphosate Tolerance Mechanism in Pitted Morningglory (Ipomoea lacunosa L.) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE absorption; S-enolpyriuvylshikimate-3-phosphate synthase (EPSPS); glyphosate; herbicide; resistance; Ipomoea lacunosa; metabolism; pitted morningglory; translocation ID RYEGRASS LOLIUM-MULTIFLORUM; AMINOMETHYLPHOSPHONIC ACID; CONYZA-CANADENSIS; RESISTANCE; TRANSLOCATION; HERBICIDE; ABSORPTION; WEED; ACCUMULATION; EVOLUTION AB Natural tolerance of Ipomoea lacunosa to glyphosate has made it problematic in the southeastern U.S. since the adoption of glyphosate-resistant crops. Experiments were conducted to determine (i) the variability in tolerance to glyphosate among accessions, (ii) if there is any correlation between metabolism of glyphosate to aminomethylphosponic acid (AMPA) or sarcosine and the level of tolerance, and (iii) the involvement of differential translocation in tolerance to glyphosate. Fourteen I. lacunosa accessions had GR50 values ranging from 58 to 151 grams of acid equivalent per hectare (ae/ha) glyphosate, a 2.6-fold variability in tolerance to glyphosate. There was no evidence of the most tolerant (MT) accession metabolizing glyphosate to AMPA more rapidly than the least tolerant (LT) accession. Metabolism to sarcosine was not found. C-14-glyphosate absorption was similar in the two accessions. LT accession translocated more C-14-glyphosate than MT accession at 24 and 48 h after treatment. Differential translocation partly explains glyphosate tolerance in MT accession. C1 [Ribeiro, Daniela N.; Shaw, David R.] Mississippi State Univ, Dept Plant & Soil Sci, Mississippi State, MS 39762 USA. [Nandula, Vijay K.; Reddy, Krishna N.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Dayan, Franck E.; Rimando, Agnes M.; Duke, Stephen O.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. RP Nandula, VK (reprint author), USDA ARS, Crop Prod Syst Res Unit, POB 350, Stoneville, MS 38776 USA. EM vijay.nandula@ars.usda.gov RI Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS) [6402-21000-050-00D]; Monsanto Co. FX This work was supported by the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) project number 6402-21000-050-00D. Funding was also provided by Monsanto Co. NR 42 TC 4 Z9 4 U1 2 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 18 PY 2015 VL 63 IS 6 BP 1689 EP 1697 DI 10.1021/jf5055722 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CB7LB UT WOS:000349807400003 PM 25625294 ER PT J AU Chandran, M Chu, Y Maleki, SJ Ozias-Akins, P AF Chandran, Manju Chu, Ye Maleki, Soheila J. Ozias-Akins, Peggy TI Stability of Transgene Expression in Reduced Allergen Peanut (Arachis hypogaea L.) across Multiple Generations and at Different Soil Sulfur Levels SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Arachis hypogaea; transgene stability; sulfur nutrition; allergen expression ID ARA H 2; SEED STORAGE PROTEINS; PISUM-SATIVUM; NITRATE REDUCTASE; IGE BINDING; AMINO-ACIDS; NITROGEN; DEFICIENCY; AVAILABILITY; PLANTS AB Transgenic peanut (Arachis hypogaea L.) containing a gene designed for RNA interference (RNAi) showed stable complete silencing of Ara h 2 and partial silencing of Ara h 6, two potent peanut allergens/proteins, along with minimal collateral changes to other allergens, Ara h 1 and Ara h 3, across three generations (T-3, T-4, and T-5) under field conditions. Different soil sulfur levels (0.012, 0.3, and 3.0 mM) differentially impacted sulfur-rich (Ara h 2, Ara h 3, and Ara h 6) versus sulfur-poor (Ara h 1) proteins in non-transgenic versus transgenic peanut. The sulfur level had no effect on Ara h 1, whereas low sulfur led to a significant reduction of Ara h 3 in transgenic and non-transgenic seeds and Ara h 2 and Ara h 6 in non-transgenic but not in transgenic peanuts because these proteins already were reduced by gene silencing. These results demonstrate stability of transgene expression and the potential utility of RNAi in allergen manipulation. C1 [Chandran, Manju; Chu, Ye; Ozias-Akins, Peggy] Univ Georgia, Dept Hort, Tifton, GA 31793 USA. [Maleki, Soheila J.] ARS, Southern Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Ozias-Akins, P (reprint author), Univ Georgia, Dept Hort, Tifton, GA 31793 USA. EM pozias@uga.edu FU Peanut Foundation; National Peanut Board; Agriculture and Food Research Initiative Competitive Grant of the USDA National Institute of Food and Agriculture [2010-85117-20550] FX This work was supported by The Peanut Foundation, The National Peanut Board, and The Agriculture and Food Research Initiative Competitive Grant 2010-85117-20550 of the USDA National Institute of Food and Agriculture. NR 67 TC 1 Z9 1 U1 3 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 18 PY 2015 VL 63 IS 6 BP 1788 EP 1797 DI 10.1021/jf504892f PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CB7LB UT WOS:000349807400013 PM 25616282 ER PT J AU Sun, JH Kou, LP Geng, P Huang, HL Yang, TB Luo, YG Chen, P AF Sun, Jianghao Kou, Liping Geng, Ping Huang, Huilian Yang, Tianbao Luo, Yaguang Chen, Pei TI Metabolomic Assessment Reveals an Elevated Level of Glucosinolate Content in CaCl2 Treated Broccoli Microgreens SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE broccoli microgreens; metabolomics; glucosinolates; food quality; UHPLC-HRMS ID TANDEM MASS-SPECTROMETRY; L. VAR.-ITALICA; LIQUID-CHROMATOGRAPHY; ION-TRAP; MUSTARD GREENS; QUALITY; BRASSICA; SPROUTS; PRODUCTS; CALCIUM AB Preharvest calcium application has been shown to increase broccoli microgreen yield and extend shelf life. In this study, we investigated the effect of calcium application on its metabolome using ultra-high-performance liquid chromatography with mass spectrometry. The data collected were analyzed using principal component analysis and orthogonal projection to latent structural discriminate analysis. Chemical composition comparison shows that glucosinolates, a very important group of phytochemicals, are the major compounds enhanced by preharvest treatment with 10 mM calcium chloride (CaCl2). Aliphatic glucosinolates (glucoerucin, glucoiberin, glucoiberverin, glucoraphanin, pentyl glucosinolate, and hexyl glucosinolate) and indolic glucosinolates (glucobrassicin, neoglucobrassicin, and 4-hydroxyglucobrassicin) were increased significantly in the CaCl2 treated microgreens using metabolomic approaches. Targeted glucosinolate analysis using the ISO 9167-1 method was further employed to confirm the findings. Results indicate that glucosinolates can be considered as a class of compounds that are responsible for the difference between two groups and a higher glucosinolate level was found in CaCl2 treated groups at each time point after harvest in comparison with the control group. C1 [Sun, Jianghao; Geng, Ping; Huang, Huilian; Chen, Pei] ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, USDA, Beltsville, MD 20705 USA. [Kou, Liping; Yang, Tianbao; Luo, Yaguang] ARS, Beltsville Agr Res Ctr, Food Qual Lab, USDA, Beltsville, MD 20705 USA. [Kou, Liping] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Huang, Huilian] Jiangxi Univ Tradit Chinese Med, Minist Educ, Key Lab Modern Preparat TCM, Nanchang 330003, Jiangxi, Peoples R China. RP Chen, P (reprint author), ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, USDA, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements at the National Institutes of Health FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture, an Interagency Agreement with the Office of Dietary Supplements at the National Institutes of Health. NR 53 TC 6 Z9 6 U1 2 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 18 PY 2015 VL 63 IS 6 BP 1863 EP 1868 DI 10.1021/jf504710r PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CB7LB UT WOS:000349807400023 PM 25594226 ER PT J AU Bellaloui, N Bruns, HA Abbas, HK Mengistu, A Fisher, DK Reddy, KN AF Bellaloui, Nacer Bruns, H. Arnold Abbas, Hamed K. Mengistu, Alemu Fisher, Daniel K. Reddy, Krishna N. TI Agricultural practices altered soybean seed protein, oil, fatty acids, sugars, and minerals in the Midsouth USA SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE seed protein; fatty acids; sugars; minerals; twin-rows; seeding rate; planting date ID AZOMETHINE-H METHOD; PLANTING DATE; PRODUCTION SYSTEM; HIGH-TEMPERATURE; CROPPING SYSTEM; SOLAR-RADIATION; MATURITY; BORON; YIELD; LINES AB Information on the effects of management practices on soybean seed composition is scarce. Therefore, the objective of this research was to investigate the effects of planting date (PD) and seeding rate (SR) on seed composition (protein, oil, fatty acids, and sugars) and seed minerals (B, P and Fe) in soybean grown in two row-types (RTs) on the Mississippi Delta region of the Midsouth USA. Two field experiments were conducted in 2009 and 2010 on Sharkey clay and Beulah fine sandy loam soil at Stoneville, MS, USA, under irrigated conditions. Soybean were grown in 102 cm single-rows and 25 cm twin-rows in 102 cm centers at SRs of 20, 30, 40, and 50 seeds m(-2). The results showed that in May and June planting, protein, glucose, P and B concentrations increased with increased SR, but at the highest SRs (40 and 50 seeds m(-2)), the concentrations remained constant or declined. Palmitic, stearic, and linoleic acid concentrations were the least responsive to SR increases. Early planting resulted in higher oil, oleic acid, sucrose, B, and P on both single and twin-rows. Late planting resulted in higher protein and linolenic acid, but lower oleic acid and oil concentrations. The changes in seed constituents could be due to changes in environmental factors (drought and temperature), and nutrient accumulation in seeds and leaves. The increase of stachyose sugar in 2010 may be due to a drier year and high temperature in 2010 compared to 2009; suggesting the possible role of stachyose as an environmental stress compound. Our research demonstrated that PD, SR, and RT altered some seed constituents, but the level of alteration in each year dependent on environmental factors such as drought and temperature. This information benefits growers and breeders for considering agronomic practices to select for soybean seed nutritional qualities under drought and high heat conditions. C1 [Bellaloui, Nacer] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Bruns, H. Arnold; Fisher, Daniel K.; Reddy, Krishna N.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Abbas, Hamed K.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Mengistu, Alemu] USDA ARS, Crop Genet Res Unit, Jackson, TN USA. RP Bellaloui, N (reprint author), USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM nacer.bellaloui@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service Project [6402-21220-012-00D] FX Authors are thankful to Debbie Boykin for statistics assistance, Sandra Mosley for lab assistance, and Joseph Baugh for field management. This work was partially supported by the U.S. Department of Agriculture, Agricultural Research Service Project 6402-21220-012-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 71 TC 9 Z9 9 U1 3 U2 37 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 18 PY 2015 VL 6 AR 31 DI 10.3389/fpls.2015.00031 PG 14 WC Plant Sciences SC Plant Sciences GA CB5CE UT WOS:000349644300001 PM 25741347 ER PT J AU Dautt-Castro, M Ochoa-Leyva, A Contreras-Vergara, CA Pacheco-Sanchez, MA Casas-Flores, S Sanchez-Flores, A Kuhn, DN Islas-Osuna, MA AF Dautt-Castro, Mitzuko Ochoa-Leyva, Adrian Contreras-Vergara, Carmen A. Pacheco-Sanchez, Magda A. Casas-Flores, Sergio Sanchez-Flores, Alejandro Kuhn, David N. Islas-Osuna, Maria A. TI Mango (Mangifera indica L.) cv. Kent fruit mesocarp de novo transcriptome assembly identifies gene families important for ripening SO FRONTIERS IN PLANT SCIENCE LA English DT Article ID ETHYLENE-RECEPTOR FAMILY; MULTIPLE FORMS; RNA-SEQ; FUNCTIONAL-CHARACTERIZATION; DIFFERENTIAL EXPRESSION; BETA-GALACTOSIDASE; ALPHONSO FRUIT; EXPANSIN GENE; PECTATE LYASE; TOMATO FRUIT AB Fruit ripening is a physiological and biochemical process genetically programmed to regulate fruit quality parameters like firmness, flavor, odor and color, as well as production of ethylene in climacteric fruit. In this study, a transcriptomic analysis of mango (Mangifera indica L.) mesocarp cv. "Kent" was done to identify key genes associated with fruit ripening. Using the Illumina sequencing platform, 67,682,269 clean reads were obtained and a transcriptome of 4.8 Gb. A total of 33,142 coding sequences were predicted and after functional annotation, 25,154 protein sequences were assigned with a product according to Swiss-Prot database and 32,560 according to non-redundant database. Differential expression analysis identified 2,306 genes with significant differences in expression between mature-green and ripe mango [1,178 up-regulated and 1,128 down-regulated (FDR <= 0.05)1. The expression of 10 genes evaluated by both gRT-PCR and RNA-seq data was highly correlated (R = 0.97), validating the differential expression data from RNA-seq alone. Gene Ontology enrichment analysis, showed significantly represented terms associated to fruit ripening like "cell wall," "carbohydrate catabolic process" and "starch and sucrose metabolic process" among others. Mango genes were assigned to 327 metabolic pathways according to Kyoto Encyclopedia of Genes and Genomes database, among them those involved in fruit ripening such as plant hormone signal transduction, starch and sucrose metabolism, galactose metabolism, terpenoid backbone, and carotenoid biosynthesis. This study provides a mango transcriptome that will be very helpful to identify genes for expression studies in early and late flowering mangos during fruit ripening. C1 [Dautt-Castro, Mitzuko; Contreras-Vergara, Carmen A.; Pacheco-Sanchez, Magda A.; Islas-Osuna, Maria A.] Ctr Invest Alimentac & Desarrollo, Lab Genet & Biol Mol Plantas, Hermosillo 83304, Sonora, Mexico. [Ochoa-Leyva, Adrian] Fac Quim UNAM, Unidad Genom Poblac, Inst Nacl Med Genom, Mexico City, DF, Mexico. [Casas-Flores, Sergio] Inst Potosino Invest Cient & Tecnol, Div Biol Mol, Lab Genom Func & Comparat, San Luis Potosi, Mexico. [Sanchez-Flores, Alejandro] Univ Nacl Autonoma Mexico, Inst Biotecnol, Unidad Univ Secuenciac Mas DNA, Cuernavaca 62191, Morelos, Mexico. [Kuhn, David N.] ARS, USDA, Subtrop Hort Res Stn, Miami, FL USA. RP Islas-Osuna, MA (reprint author), Ctr Invest Alimentac & Desarrollo, Lab Genet & Biol Mol Plantas, Carretera Ejido La Victoria Km 0-6, Hermosillo 83304, Sonora, Mexico. EM islasosu@ciad.mx RI Sanchez-Flores, Alejandro/L-9454-2016 OI Sanchez-Flores, Alejandro/0000-0003-0476-3139 FU CONACyT; CONACyT [CB2012-01-178296] FX MDC and MAPS were supported by a PhD scholarship from CONACyT. Dr. MAIO acknowledges grant CB2012-01-178296 from CONACyT. We thank CIAD personnel Gerardo Reyna, Felipe Isaac, Luis Leyva, Adalberto Murrieta, Jose Luis Aguilar, and Martin Peralta for bibliographical and computational support; Monica Villegas, Emmanuel Aispuro, and Francisco Soto for laboratory technical support. We thank Zyanya Lucia Zatarain Barron and Ma. Fernanda Cornejo Granados for the statistical. correlation analysis. We thank the services provided by Unidad Universitaria de Secuenciacion Masiva de DNA from IBT-UNAM. We thank Arnulfo Ochoa for computational support and Ing. Ernesto Sanchez from INIFAP for access to mango samples and to Veronica Jimenez for uploading data, We also thank Dr, Frans Tax (University of Arizona) and Dr. Rogerio Sotelo-Mundo (CIAD) for critical reading of the manuscript. NR 63 TC 5 Z9 7 U1 10 U2 49 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD FEB 18 PY 2015 VL 6 AR 62 DI 10.3389/fpls.2015.00062 PG 12 WC Plant Sciences SC Plant Sciences GA CB5CD UT WOS:000349644200002 PM 25741352 ER PT J AU Craig, AM Klotz, JL Duringer, JM AF Craig, A. Morrie Klotz, James L. Duringer, Jennifer M. TI Cases of ergotism in livestock and associated ergot alkaloid concentrations in feed SO FRONTIERS IN CHEMISTRY LA English DT Article DE ergot; ergotism; ergovaline; ergotamine; ergocornine; clinical disease; saphenous vein; cattle ID LATERAL SAPHENOUS VEINS; TALL FESCUE; HPLC METHOD; ERGOVALINE; RECEPTORS; MYCOTOXINS; ENDOPHYTES; EXPOSURE; BIOASSAY AB Ergot-induced disease in humans was known long before Biblical times and has been the root cause for countless human epidemics spanning from the early fourteenth century to the late sixteenth century. In contrast, many of these same ergot alkaloids have been utilized for their medicinal properties to mitigate migraine headaches and have had indications as anti-carcinogens. Although ergot alkaloids have been used for centuries by humans, basic pharmacokinetic data has not been documented for clinical disease in livestock. Consequently, a threshold dose and accurate dose-response data have yet to be established. Throughout the past several years, new detection techniques have emerged to detect these alkaloids at the parts per billion (ppb) level which has allowed for new efforts to be made with respect to determining threshold levels and making accurate clinical diagnoses in affected animals. This perspectives article provides a critical initial step for establishing a uniform interpretation of ergot toxicosis from limited existing data. C1 [Craig, A. Morrie] Oregon State Univ, Coll Vet Med, Dept Biomed Sci, 105 Magruder Hall, Corvallis, OR 97331 USA. [Klotz, James L.] ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY USA. [Duringer, Jennifer M.] Oregon State Univ, Dept Environm & Mol Toxicol, Corvallis, OR 97331 USA. RP Craig, AM (reprint author), Oregon State Univ, Coll Vet Med, Dept Biomed Sci, 105 Magruder Hall, Corvallis, OR 97331 USA. EM a.morrie.craig@oregonstate.edu NR 43 TC 3 Z9 3 U1 3 U2 5 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND EI 2296-2646 J9 FRONT CHEM JI Front. Chem. PD FEB 18 PY 2015 VL 3 AR 8 DI 10.3389/fchem.2015.00008 PG 6 WC Chemistry, Multidisciplinary SC Chemistry GA DI1VM UT WOS:000373284600001 PM 25741505 ER PT J AU Westbrook, JK Suh, CPC Yang, CH Lan, YB Eyster, RS AF Westbrook, John K. Suh, Charles P. -C. Yang, Chenghai Lan, Yubin Eyster, Ritchie S. TI Airborne multispectral detection of regrowth cotton fields SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE remote sensing; boll weevils; Anthonomus grandis; pest management; eradication ID REMOTELY-SENSED DATA; CROP YIELD; IMAGERY; PLANT AB Effective methods are needed for timely areawide detection of regrowth cotton plants because boll weevils (a quarantine pest) can feed and reproduce on these plants beyond the cotton production season. Airborne multispectral images of regrowth cotton plots were acquired on several dates after three shredding (i.e., stalk destruction) dates. Linear spectral unmixing (LSU) classification was applied to high-resolution airborne multispectral images of regrowth cotton plots to estimate the minimum detectable size and subsequent growth of plants. We found that regrowth cotton fields can be identified when the mean plant width is similar to 0.2 m for an image resolution of 0.1 m. LSU estimates of canopy cover of regrowth cotton plots correlated well (r(2) = 0.81) with the ratio of mean plant width to row spacing, a surrogate measure of plant canopy cover. The height and width of regrowth plants were both well correlated (r(2) = 0.94) with accumulated degree-days after shredding. The results will help boll weevil eradication program managers use airborne multispectral images to detect and monitor the regrowth of cotton plants after stalk destruction, and identify fields that may require further inspection and mitigation of boll weevil infestations. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. C1 [Westbrook, John K.; Suh, Charles P. -C.; Yang, Chenghai; Lan, Yubin; Eyster, Ritchie S.] USDA ARS, Insect Control & Cotton Dis Res Unit, College Stn, TX 77845 USA. RP Westbrook, JK (reprint author), USDA ARS, Insect Control & Cotton Dis Res Unit, Route 5,2771 F & B Rd, College Stn, TX 77845 USA. EM john.westbrook@ars.usda.gov FU Cotton Foundation [5002-009-00]; Cotton Inc. [10-776] FX We thank the Cotton Foundation (general project #5002-009-00) and Cotton Inc. (core project #10-776) for funding this project, and Mike O'Neil, Parker Knutson, Fred Gomez, Lee Denham, and Cody Wall for their technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 22 TC 0 Z9 0 U1 1 U2 9 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD FEB 17 PY 2015 VL 9 AR 096081 DI 10.1117/1.JRS.9.096081 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CC6MG UT WOS:000350478500001 ER PT J AU Lu, ZJ Breidt, F AF Lu, Zhongjing Breidt, Fred TI Escherichia coli O157:H7 bacteriophage Phi 241 isolated from an industrial cucumber fermentation at high acidity and salinity SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE bacteriophage; phage Phi 241; Escherichia coli O157:H7; biocontrol agent; cucumber fermentation; high acidity and salinity; food safety ID HEMOLYTIC-UREMIC-SYNDROME; LACTOBACILLUS-PLANTARUM BACTERIOPHAGE; SALMONELLA-ENTERICA; APPLE CIDER; LISTERIA-MONOCYTOGENES; VIRULENT BACTERIOPHAGE; WATERBORNE OUTBREAK; O157-H7 INFECTIONS; GENOMIC ANALYSIS; BLOODY DIARRHEA AB A novel phage, Phi 241, specific for Escherichia coli O157:H7 was isolated from an industrial cucumber fermentation where both acidity (pH <= 3.7) and salinity (>= 5% NaCl) were high. The phage belongs to the Myoviridae family. Its latent period was 15 min and average burst size was 53 phage particles per infected cell. The phage was able to lyse 48 E. coli O157:H7 strains, but none of the 18 non-O157 strains (including E. coli O104:H7) or the 2 O antigen-negative mutants of O157:H7 strain, 43895 Delta per (also lacking H7 antigen) and F12 (still expressing H7 antigen). However, the phage was able to lyse a per-complemented strain (43895 Delta perComp) which expresses O157 antigen. These results indicated that phage Phi 241 is specific for O157 antigen, and E. coli strains lacking O157 antigen were resistant to the phage infection, regardless of the presence or absence of H7 antigen. SDS-PAGE profile revealed at least 13 structural proteins of the phage. The phage DNA was resistant to many commonly used restriction endonucleases, suggesting the presence of modified nucleotides in the phage genome. At the multiplicity of infection of 10, 3, or 0.3, the phage caused a rapid cell lysis within 1 or 2 h, resulting in 3.5- or 4.5-log-unit reduction in cell concentration. The high lytic activity, specificity and tolerance to low pH and high salinity make phage Phi 241 a potentially ideal biocontrol agent of E. coli O157:H7 in various foods. To our knowledge, this is the first report on E. coli O157:H7 phage isolated from high acidity and salinity environment. C1 [Lu, Zhongjing] Kennesaw State Univ, Dept Mol & Cellular Biol, Kennesaw, GA 30144 USA. [Breidt, Fred] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, USDA Agr Res Serv, Raleigh, NC 27695 USA. RP Lu, ZJ (reprint author), Kennesaw State Univ, Dept Mol & Cellular Biol, Kennesaw, GA 30144 USA. EM jean_lu@kennesaw.edu NR 98 TC 5 Z9 5 U1 0 U2 7 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 17 PY 2015 VL 6 AR 67 DI 10.3389/fmicb.2015.00067 PG 10 WC Microbiology SC Microbiology GA CC3LW UT WOS:000350251600001 PM 25741324 ER PT J AU Zunino, SJ Storms, DH AF Zunino, Susan J. Storms, David H. TI Physiological Levels of Resveratrol Metabolites are Ineffective as Anti-Leukemia Agents Against Jurkat Leukemia Cells SO NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL LA English DT Article ID MITOCHONDRIAL-MEMBRANE PROTEIN; TRANS-RESVERATROL; DIETARY RESVERATROL; IN-VIVO; CANCER; GROWTH; APOPTOSIS; MICE; MITONEET; RATS AB Dietary resveratrol is metabolically transformed in vivo by the intestine and liver to produce resveratrol glucuronides and sulfates in humans. Little is known about the anticancer activities of these metabolic products. The majority of in vitro studies have investigated effects of resveratrol aglycone at supraphysiological levels. Physiological levels of resveratrol-3-O-glucuronide, resveratrol-4 '-O-glucuronide, and resveratrol-3-O-sulfate, the major in vivo metabolites of dietary resveratrol, were evaluated as anticancer agents against Jurkat T leukemia cells. Propidium iodide was use to measure cell death and changes in cell cycle, and the mitochondrial membrane dye JC-1 was used to measure changes in mitochondrial membrane potential by flow cytometry. PKH67 was used to evaluate changes in proliferation of the cells by flow cytometry. Jurkat cells were exposed to 0, 2.5, 5, 10, 15, and 20 mu M of each resveratrol metabolite, which are concentrations achievable in vivo. None of the resveratrol metabolites were able to kill Jurkat T leukemia cells or alter cell cycle or proliferation at these concentrations. Only resveratrol-3-O-sulfate induced depolarization of mitochondrial membranes but without induction of cell death. These results suggest that the in vivo transformation of resveratrol to these glucuronide and sulfate metabolites renders these agents ineffective against T leukemia cells. C1 [Zunino, Susan J.; Storms, David H.] ARS, Immun & Dis Prevent Unit, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. RP Zunino, SJ (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM susan.zunino@ars.usda.gov FU United States Department of Agriculture (USDA) Current Research Information System project [5306-51530-018-00D] FX This study was supported by the United States Department of Agriculture (USDA) Current Research Information System project no. 5306-51530-018-00D. USDA is an equal opportunity provider and employer. NR 40 TC 0 Z9 0 U1 0 U2 2 PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND SN 0163-5581 EI 1532-7914 J9 NUTR CANCER JI Nutr. Cancer PD FEB 17 PY 2015 VL 67 IS 2 BP 266 EP 274 DI 10.1080/01635581.2015.989373 PG 9 WC Oncology; Nutrition & Dietetics SC Oncology; Nutrition & Dietetics GA CC4QP UT WOS:000350338300009 PM 25622018 ER PT J AU Mauri, M Farina, M Patriarca, G Simonutti, R Klasson, KT Cheng, HN AF Mauri, Michele Farina, Matteo Patriarca, Giorgio Simonutti, Roberto Klasson, K. Thomas Cheng, H. N. TI Xe-129 NMR Studies of Pecan Shell-Based Biochar and Structure-Process Correlations SO INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION LA English DT Article DE Pecan shells; Biochar; Agricultural by-products; Activated carbon; Xe-129 NMR ID PHOSPHORIC-ACID ACTIVATION; HYPERPOLARIZED XENON; CARBONS; SPECTROSCOPY; ADSORPTION; AMENDMENT; SOLIDS; SOIL AB Pecan shell-based biochar is utilized as a filtration medium, sequestrant for metallic ions, soil conditioner, and other applications. One process for creating the biochar involves the use of phosphoric acid at high temperature in a partial oxygen atmosphere to produce a highly porous carbonaceous material. In this work, we found Xe-129 NMR to be an excellent technique to study micropores in biochar. Thus, the Xe-129 chemical shift in biochar was found to vary linearly with the xenon pressure; from the data an estimate of about 8-9 angstrom could be proposed for the average pore diameter in pecan shell-based biochar. Through saturation recovery and 2-D NMR exchange experiments, information on the exchange between free versus bound xenon was obtained. Furthermore, correlations of Xe-129 NMR data with the carbonization process conditions were made. C1 [Mauri, Michele; Farina, Matteo; Patriarca, Giorgio; Simonutti, Roberto] Univ Milano Bicocca, Dept Mat Sci, I-20125 Milan, Italy. [Klasson, K. Thomas; Cheng, H. N.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Mauri, M (reprint author), Univ Milano Bicocca, Dept Mat Sci, Via R Cozzi 55, I-20125 Milan, Italy. EM michele.mauri@mater.unimib.it; hn.cheng@ars.usda.gov RI Mauri, Michele/A-3561-2013; OI Mauri, Michele/0000-0002-7777-9820; Klasson, K. Thomas/0000-0003-3358-3081 NR 30 TC 1 Z9 1 U1 3 U2 29 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1023-666X EI 1563-5341 J9 INT J POLYM ANAL CH JI Int. J. Polym. Anal. Charact. PD FEB 17 PY 2015 VL 20 IS 2 BP 119 EP 129 DI 10.1080/1023666X.2015.979038 PG 11 WC Polymer Science SC Polymer Science GA CA5EK UT WOS:000348931000003 ER PT J AU Calvo, AM Cary, JW AF Calvo, Ana M. Cary, Jeffrey W. TI Association of fungal secondary metabolism and sclerotial biology SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE secondary metabolism; genetic regulation; sclerotia; VeA; velvet; Aspergillus; gene cluster; morphogenesis ID BIOSYNTHESIS GENE CLUSTERS; MATING-TYPE GENES; ASPERGILLUS-FLAVUS; AFLATOXIN BIOSYNTHESIS; CYCLOPIAZONIC ACID; SEXUAL DEVELOPMENT; OXIDATIVE STRESS; FUSARIUM-VERTICILLIOIDES; TRANSCRIPTION FACTOR; MYCOTOXIN PRODUCTION AB Fungal secondary metabolism and morphological development have been shown to be intimately associated at the genetic level. Much of the literature has focused on the co-regulation of secondary metabolite production (e.g., sterigmatocystin and aflatoxin in Aspergillus nidulans and Aspergillus flavus, respectively) with conidiation or formation of sexual fruiting bodies. However, many of these genetic links also control sclerotial production. Sclerotia are resistant structures produced by a number of fungal genera. They also represent the principal source of primary inoculum for some phytopathogenic fungi. In nature, higher plants often concentrate secondary metabolites in reproductive structures as a means of defense against herbivores and insects. By analogy, fungi also sequester a number of secondary metabolites in sclerotia that act as a chemical defense system against fungivorous predators. These include antiinsectant compounds such as tetramic acids, indole diterpenoids, pyridones, and diketopiperazines. This chapter will focus on the molecular mechanisms governing production of secondary metabolites and the role they play in sclerotial development and fungal ecology, with particular emphasis on Aspergillus species. The global regulatory proteins VeA and LaeA, components of the velvet nuclear protein complex, serve as virulence factors and control both development and secondary metabolite production in many Aspergillus species. We will discuss a number of VeA- and LaeA-regulated secondary metabolic gene clusters in A. flavus that are postulated to be involved in sclerotial morphogenesis and chemical defense. The presence of multiple regulatory factors that control secondary metabolism and sclerotial formation suggests that fungi have evolved these complex regulatory mechanisms as a means to rapidly adapt chemical responses to protect sclerotia from predators, competitors and other environmental stressors. C1 [Calvo, Ana M.] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. [Cary, Jeffrey W.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. RP Calvo, AM (reprint author), No Illinois Univ, Dept Biol Sci, 155 Castle Rd, De Kalb, IL 60115 USA. EM amcalvo@niu.edu NR 173 TC 14 Z9 15 U1 13 U2 69 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 16 PY 2015 VL 6 AR 62 DI 10.3389/fmicb.2015.00062 PG 16 WC Microbiology SC Microbiology GA CC3KZ UT WOS:000350248900001 PM 25762985 ER PT J AU Munoz-Gutierrez, JF Schneider, DA Baszler, TV Greenlee, JJ Nicholson, EM Stanton, JB AF Munoz-Gutierrez, Juan F. Schneider, David A. Baszler, Timothy V. Greenlee, Justin J. Nicholson, Eric M. Stanton, James B. TI hTERT-immortalized ovine microglia propagate natural scrapie isolates SO VIRUS RESEARCH LA English DT Article DE hTERT; Immortalization; Microglia; Prion; Scrapie ID CHRONIC-WASTING-DISEASE; PRION PROTEIN; PRPSC ACCUMULATION; DENDRITIC CELLS; SHEEP SCRAPIE; REPLICATION; INFECTION; ASSAY; DIAGNOSIS; STRAINS AB Ex vivo propagation of natural prion isolates (i.e., propagated solely in the natural host) is crucial for the characterization and study of transmissible spongiform encephalopathies (TSEs). Several well-established, prion-permissive cell culture systems are available; however, only a few cell lines are permissive to natural prion isolates and these cells are not pathophysiologically relevant (e.g., renal epithelium and fibroblast-like cells). Therefore, a pathophysiologically relevant cell line derived from a natural TSE host could be used for propagation of natural prion isolates. In this study, ovine brain macrophages (microglia) were immortalized by transfection with the human telomerase reverse transcriptase (hTERT) gene to identify cell lines (hTERT-microglia) permissive to natural scrapie prion isolates. Following transfection, hTERT-microglia were passaged up to 100 times and their lifespan was significantly longer compared to parental cells (Fisher's exact test, P < 0.001). Multiple sublines were permissive to cell culture-adapted prions; two sublines were also permissive to natural scrapie isolates (i.e., derived from brain homogenates of sheep infected with scrapie). Prion infectivity and partial protease resistance of the prion protein were maintained in hTERT-microglia. Comparisons between scrapie-permissive and non-permissive hTERT-microglia sublines revealed that overall quantity of the normal cellular prion protein was not associated with prion permissiveness. The use of hTERT-microglia in future TSE studies may be more germane to the characterization of the cellular and subcellular pathophysiology of natural scrapie prion isolates and to investigate host-specific factors involved in prion replication. (C) 2015 Elsevier B.V. All rights reserved. C1 [Munoz-Gutierrez, Juan F.; Schneider, David A.; Baszler, Timothy V.; Stanton, James B.] Washington State Univ, Coll Vet Med, Dept Microbiol & Pathol, Pullman, WA 99164 USA. [Schneider, David A.] ARS, USDA, Pullman, WA 99164 USA. [Greenlee, Justin J.; Nicholson, Eric M.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Stanton, JB (reprint author), Univ Georgia, Dept Pathol, Coll Vet Med, 501 DW Brooks Dr, Athens, GA 30602 USA. EM jmunoz@vetmed.wsu.edu; das@vetmed.wsu.edu; baszlert@vetmed.wsu.edu; justin.greenlee@ars.usda.gov; eric.nicholson@ars.usda.gov; jbs@uga.edu RI Stanton, James/A-5277-2011; OI Stanton, James/0000-0002-7661-2631; Schneider, David/0000-0001-9659-6731 FU WSU Office of Research; USDA-ARS; WSU CVM Intramural Research Program (Robert R. Fast Endowed Food Animal Research Award); WSU CVM Intramural Research Program (Stanley L. Adler Research Fund) FX We thank Robert A. Weinberg and Hartmut K. Land for providing plasmids constructs, Didier Vilette for permission to use the Rov cells, and Byron Caughey for supplying the Roy cells and culturing methods. This work was supported by the WSU Office of Research, USDA-ARS, and the WSU CVM Intramural Research Program (Robert R. Fast Endowed Food Animal Research Award and Stanley L. Adler Research Fund). We thank Edith Orozco (USDA-ARS) for her excellent technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 38 TC 3 Z9 3 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD FEB 16 PY 2015 VL 198 BP 35 EP 43 DI 10.1016/j.virusres.2014.10.028 PG 9 WC Virology SC Virology GA CC9QQ UT WOS:000350706100006 PM 25592246 ER PT J AU Turlapati, SA Minocha, R Long, S Ramsdell, J Minocha, SC AF Turlapati, Swathi A. Minocha, Rakesh Long, Stephanie Ramsdell, Jordan Minocha, Subhash C. TI Oligotyping reveals stronger relationship of organic soil bacterial community structure with N-amendments and soil chemistry in comparison to that of mineral soil at Harvard Forest MA, USA SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE bacterial community; forest soils; microbiome; oligotypes; pyrosequencing; QIIME software; OTUs; entropy ID CHRONIC NITROGEN ADDITIONS; BROOK EXPERIMENTAL FOREST; LONG-TERM FERTILIZATION; 16S RIBOSOMAL-RNA; MICROBIAL COMMUNITIES; FUNGAL COMMUNITIES; RARE BIOSPHERE; RED SPRUCE; DIVERSITY; ECOSYSTEM AB The impact of chronic nitrogen amendments on bacterial communities was evaluated at Harvard Forest, Petersham, MA, USA. Thirty soil samples (3 treatments x 2 soil horizons x 5 subplots) were collected in 2009 from untreated (control), low nitrogen-amended (LN; 50 kg NH4NO3 ha(-1) yr(-1)) and high nitrogen-amended (HN; 150 kg NH4NO3 ha(-1) yr(-1)) plots. PCR-amplified partial 16S rRNA gene sequences made from soil DNA were subjected to pyrosequencing (Turlapati Mal., 2013) and analyses using oligotyping. The parameters M (the minimum count of the most abundant unique sequence in an oligotype) and s (the minimum number of samples in which an oligotype is expected to be present) had to be optimized for forest soils because of high diversity and the presence of rare organisms. Comparative analyses of the pyrosequencing data by oligotyping and operational taxonomic unit clustering tools indicated that the former yields more refined units of taxonomy with sequence similarity of >= 99.5%. Sequences affiliated with four new phyla and 73 genera were identified in the present study as compared to 27 genera reported earlier from the same data (Tudapati et al., 2013). Significant rearrangements in the bacterial community structure were observed with N-amendments revealing the presence of additional genera in N-amended plots with the absence of some that were present in the control plots. Permutational MANOVA analyses indicated significant variation associated with soil horizon and N treatment for a majority of the phyla. In most cases soil horizon partitioned more variation relative to treatment and treatment effects were more evident for the organic (Org) horizon. Mantel test results for Org soil showed significant positive correlations between bacterial communities and most soil parameters including NH4 and NO3. In mineral soil, correlations were seen only with pH, NH4, and NO3. Regardless of the pipeline used, a major hindrance for such a study remains to be the lack of reference databases for forest soils. C1 [Turlapati, Swathi A.; Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Turlapati, Swathi A.; Minocha, Rakesh; Long, Stephanie] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Ramsdell, Jordan] Univ New Hampshire, Hubbard Ctr Genome Studies, Durham, NH 03824 USA. RP Minocha, R (reprint author), US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH 03824 USA. EM rminocha@unh.edu FU New Hampshire Agricultural Experiment Station FX The authors are grateful to Kenneth R. Dudzik for his technical assistance. They also extend their thanks to Dr. Murat Eren for providing guidance in using the oligotyping pipeline. Partial funding was provided by the New Hampshire Agricultural Experiment Station. This is Scientific Contribution Number 2568. NR 81 TC 1 Z9 1 U1 6 U2 24 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 16 PY 2015 VL 6 AR 49 DI 10.3389/fmicb.2015.00049 PG 16 WC Microbiology SC Microbiology GA CC3KX UT WOS:000350248600001 PM 25762982 ER PT J AU Hayes, TR Lyon, PA Barnes, CL Trabue, S Benny, PD AF Hayes, Thomas R. Lyon, Patrice A. Barnes, Charles L. Trabue, Steven Benny, Paul D. TI Influence of Functionalized Pyridine Ligands on the Radio/Chemical Behavior of [M-I(CO)(3)](+) (M = Re and Tc-99m) 2+1 Complexes SO INORGANIC CHEMISTRY LA English DT Article ID ALPHA-MSH PEPTIDE; IN-VITRO; BIOMOLECULES; CHEMISTRY; SYSTEMS; TECHNETIUM; BASICITY; NUCLEAR; DESIGN AB While a number of chelate strategies have been developed for the organometallic precursor fac-[M-I(OH2)(3)(CO)(3)](+) (M = Re, Tc-99m), a unique challenge has been to improve the overall function and performance of these complexes for in vivo and in vitro applications. Since its discovery, fac-[MI(OH2)(3)(CO)(3)](+) has served as an essential scaffold for the development of new targeted Tc-99m based radiopharmaceuticals due to its labile aquo ligands. However, the lipophilic nature of the fac-[MI(CO)(3)](+) core can influence the in vivo pharmacokinetics and biodistribution of the complexes. In an effort to understand and improve this behavior, monosubstituted pyridine ligands were used to assess the impact of donor nitrogen basicity on binding strength and stability of fac-[MI(CO)(3)](+) in a 2 + 1 labeling strategy. A series of Re and Tc-99m complexes were synthesized with picolinic acid as a bidentate ligand and 4-substituted pyridine ligands. These complexes were designed to probe the effect of pK(a) from the monodentate pyridine ligand both at the macro scale and radiochemical concentrations. Comparison of X-ray structural data and radiochemical solution experiments clearly indicate an increase in overall yield and stability as pyridine basicity increased. C1 [Hayes, Thomas R.; Lyon, Patrice A.; Benny, Paul D.] Washington State Univ, Dept Chem, Pullman, WA 99164 USA. [Barnes, Charles L.] Univ Missouri, Dept Chem, Columbia, MO 65211 USA. [Trabue, Steven] USDA, Natl Soil Tilth Lab, Ames, IA 50011 USA. RP Benny, PD (reprint author), Washington State Univ, Dept Chem, Pullman, WA 99164 USA. EM bennyp@wsu.edu FU DOE, Radiochemistry and Radiochemistry Instrumentation Program [DE-FG02-08-ER64672]; Washington State University FX This worked was funded in part by the DOE, Radiochemistry and Radiochemistry Instrumentation Program (#DE-FG02-08-ER64672) and Washington State University. NR 30 TC 4 Z9 4 U1 1 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0020-1669 EI 1520-510X J9 INORG CHEM JI Inorg. Chem. PD FEB 16 PY 2015 VL 54 IS 4 BP 1528 EP 1534 DI 10.1021/ic5025208 PG 7 WC Chemistry, Inorganic & Nuclear SC Chemistry GA CB5GX UT WOS:000349656600042 PM 25590985 ER PT J AU Song, MH Bai, YL Xu, J Carter, MQ Shi, CL Shi, XM AF Song, Minghui Bai, Yalong Xu, Jie Carter, Michelle Qiu Shi, Chunlei Shi, Xianming TI Genetic diversity and virulence potential of Staphylococcus aureus isolates from raw and processed food commodities in Shanghai SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Staphylococcus aureus; Multi Locus Sequence Typing (MLST); agr typing; Toxin genes; mecA; Food commodities ID PANTON-VALENTINE LEUKOCIDIN; METHICILLIN-RESISTANT; MOLECULAR ANALYSIS; TOXIN GENES; AGR; ENTEROTOXIN; STRAINS; CHINA; IDENTIFICATION; HUMANS AB The risk of zoonotic transmission to humans highlights the need to understand the molecular ecology of Staphylococcus aureus in foods. In this study, 142 S. aureus isolates obtained from various raw and processed foods from Shanghai, China were characterized to determine their genetic diversity and virulence gene content. A total of 16 clonal complexes (CCs), 34 staphylococcal protein A (spa) types, and 6 accessory gene regulator (agr) allelic groups were identified and analyzed among the 142 S. aureus isolates. Among these, the genotype CC188-t189-agr I was the most prevalent, constituting 28.2% of all isolates. The presence of virulence genes encoding 20 staphylococcal enterotoxins (se), toxic shock syndrome toxin (tsst1), exfoliative toxins (eta, etb, and etd), Panton-Valentine leukocidin (lukS-PV and lukF-PV), as well as methicillin resistance gene (mecA), was determined by PCR. Of these S. aureus isolates, 72.5% harbored toxin genes, in which the most frequent toxin gene was sep (43.7%), followed by sej (26.1%) and pvl (21.1%). In contrast, see, ses, set, tsst1, etb, and etd were not found in any of the isolates tested. Eight S. aureus isolates (5.6%, 8/142), seven from raw milk and one from frozen food, were mecA positive and resistant to oxacillin, thus were MRSA. The 142S. aureus isolates displayed 52 different toxin gene profiles. Although no direct association was found between toxin gene profile and the S. aureus genotype, the isolates belonging to CC5, CC9, CC20, CC50, and CC72 clonal lineages in general carried more toxin genes (>5) compared with the isolates in other CCs. It was also revealed that raw milk and raw meat were the major sources of isolates containing multiple toxin genes. S. aureus isolates from food that were genetically highly related, displayed diverse toxin gene profiles, implying the significant role of horizontal gene transfer in the emergence of highly toxigenic S. aureus isolates. (c) 2014 Elsevier B.V. All rights reserved. C1 [Song, Minghui; Bai, Yalong; Xu, Jie; Shi, Chunlei; Shi, Xianming] Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. [Shi, Xianming] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China. [Carter, Michelle Qiu] USDA, ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. RP Shi, CL (reprint author), Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. EM clshi@sjtu.edu.cn; xmshi@sjtu.edu.cn FU Ministry of Science and Technology of China [2012AA101601, 2012BAK17B10, 2012BAD29B02]; Ministry of Education of China [20120073110052] FX This study was supported by the Ministry of Science and Technology of China (2012AA101601, 2012BAK17B10 and 2012BAD29B02) and the Ministry of Education of China (20120073110052). We also thank Dr. George Paoli from the Eastern Regional Research Center of USDA for critical reading of this manuscript. NR 46 TC 8 Z9 8 U1 1 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD FEB 16 PY 2015 VL 195 BP 1 EP 8 DI 10.1016/j.ijfoodmicro.2014.11.020 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CA6XP UT WOS:000349061100001 PM 25485928 ER PT J AU Huang, LH AF Huang, Lihan TI Dynamic determination of kinetic parameters, computer simulation, and probabilistic analysis of growth of Clostridium perfringens in cooked beef during cooling SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE C. perfringens; Dynamic modeling; Bootstrap; Optimization; Monte Carlo analysis; Numerical analysis ID PREDICTIVE MICROBIOLOGY; NUMERICAL-ANALYSIS; MODEL; TEMPERATURES; TOOL AB The objective of this research was to develop a new one-step methodology that uses a dynamic approach to directly construct a tertiary model for prediction of the growth of Clostridium perfringens in cooked beef. This methodology was based on simultaneous numerical analysis and optimization of both primary and secondary models using multiple dynamic growth curves obtained under different conditions. Once the models were constructed, the bootstrap method was used to calculate the 95% confidence intervals of kinetic parameters, and a Monte Carlo simulation method was developed to validate the models using the growth curves not previously used in model development. The results showed that the kinetic parameters obtained from this study accurately matched the common characteristics of C perfringens, with the optimum temperature being 45.3 degrees C. The results also showed that the predicted growth curves matched accurately with experimental observations used in validation. The mean of residuals of the predictions is -0.02 log CFU/g, with a standard deviation of only 0.23 log CFU/g. For relative growths <1 log CFU/g, the residuals of predictions are <0.4 log CFU/g. Overall, 74% of the residuals of predictions are <0.2 log CFU/g, 7.7% are >0.4 log CFU/g, while only 1.5% are >0.8 log CFU/g. In addition, the dynamic model also accurately predicted four isothermal growth curves arbitrarily chosen from the literature. Finally, the Monte Carlo simulation was used to provide the probability of >1 and 2 log CFU/g relative growths at the end of cooling. The results of this study will provide a new and accurate tool to the food industry and regulatory agencies to assess the safety of cooked beef in the event of cooling deviation. Published by Elsevier B.V. C1 USDA, Eastern Reg Res Ctr, Agr Res Serv, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), USDA, Eastern Reg Res Ctr, Agr Res Serv, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov OI Huang, Lihan/0000-0002-9565-4503 FU USDA/NIFA [2011-68003-20096] FX The author would like to thank Mr. Changcheng Li of Fujian Agriculture and Forestry University of conducting experiments, and Mr. Harter Barry of USDA ARS for technical assistance. This project is partially supported by a USDA/NIFA grant (2011-68003-20096). NR 27 TC 9 Z9 9 U1 5 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD FEB 16 PY 2015 VL 195 BP 20 EP 29 DI 10.1016/j.ijfoodmicro.2014.11.025 PG 10 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA CA6XP UT WOS:000349061100003 PM 25500276 ER PT J AU Zheng, RB Tshabalala, MA Li, QY Wang, HY AF Zheng, Rongbo Tshabalala, Mandla A. Li, Qingyu Wang, Hongyan TI Construction of hydrophobic wood surfaces by room temperature deposition of rutile (TiO2) nanostructures SO APPLIED SURFACE SCIENCE LA English DT Article DE Nanostructure; Rutile TiO2; Superhydrophobicity; Water uptake; Wood surface ID FILMS; GROWTH; TICL3 AB A convenient room temperature approach was developed for growing rutile TiO2 hierarchical structures on the wood surface by direct hydrolysis and crystallization of TiCl3 in saturated NaCl aqueous solution. The morphology and the crystal structure of TiO2 coated on the wood surface were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively. The TiO2 morphology on the wood surface could be tuned by simply changing either the reaction time or pH value of the reaction mixture. After modification with perfluorodecyltriethoxysilane (PFDTS), the water contact angle (WCA) of the Ti02-treated wood (T1) surface increased to 140.0 +/- 4.2 degrees, which indicated a highly hydrophobic wood surface. In addition, compared with untreated control wood, PFDTS-TiO2 treatment (PFDTS-T1-treated) not only reduced liquid water uptake, but also delayed the onset of water saturation point of the wood substrate. The weight change of PFDTS-T1-treated wood after 24h of water immersion was 19.3%, compared to 81.3% for the untreated control wood. After 867 h of water immersion, the weight change for the treated and untreated wood specimens was 117.1%, and 155.1%, respectively. The untreated control wood reached the steady state after 187 h, while the PFDTS-T1-treated wood did not reach the steady state until after 600 h of immersion. Published by Elsevier B.V. C1 [Zheng, Rongbo; Li, Qingyu] Southwest Forestry Univ, Coll Sci, Kunming 650224, Peoples R China. [Zheng, Rongbo] Southwest Forestry Univ, Wood Adhes & Glued Prod Key Lab Yunnan Prov, Kunming 650224, Peoples R China. [Tshabalala, Mandla A.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Wang, Hongyan] Zhejiang Forestry Acad, Hangzhou 310023, Zhejiang, Peoples R China. RP Zheng, RB (reprint author), Southwest Forestry Univ, Coll Sci, Kunming 650224, Peoples R China. EM zhengrbzy@hotmail.com; mtshabalala@fs.fed.us FU National Natural Science Foundation of China [31100420, 41001332]; Southwest Forestry University Foundation [110930] FX The financial support for this research was provided by the National Natural Science Foundation of China (31100420, 41001332), and the frame of research project was funded by the Southwest Forestry University Foundation (110930). NR 16 TC 6 Z9 6 U1 1 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-4332 EI 1873-5584 J9 APPL SURF SCI JI Appl. Surf. Sci. PD FEB 15 PY 2015 VL 328 BP 453 EP 458 DI 10.1016/j.apsusc.2014.12.083 PG 6 WC Chemistry, Physical; Materials Science, Coatings & Films; Physics, Applied; Physics, Condensed Matter SC Chemistry; Materials Science; Physics GA CB4RO UT WOS:000349615800058 ER PT J AU Cheng, JL Leu, SY Zhu, JY Gleisner, R AF Cheng, Jinlan Leu, Shao-Yuan Zhu, J. Y. Gleisner, Rolland TI High titer and yield ethanol production from undetoxified whole slurry of Douglas-fir forest residue using pH profiling in SPORL SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Article DE Forest residue; Enzymatic hydrolysis and fermentation; High solids processing; Fermentation inhibitors; High-titer biofuel ID ENHANCE ENZYMATIC SACCHARIFICATION; DILUTE-ACID; CELLULOSE ACCESSIBILITY; LODGEPOLE PINE; PRETREATMENT; BIOCONVERSION; LIGNIN; LIGNOCELLULOSES; HYDROLYSIS; BIOMASS AB Background: Forest residue is one of the most cost-effective feedstock for biofuel production. It has relatively high bulk density and can be harvested year round, advantageous for reducing transportation cost and eliminating onsite storage. However, forest residues, especially those from softwood species, are highly recalcitrant to biochemical conversion. A severe pretreatment for removing this recalcitrance can result in increased sugar degradation to inhibitors and hence cause difficulties in fermentation at high solid loadings. Here, we presented high titer ethanol production from Douglas-fir forest residue without detoxification. The strong recalcitrance of the Douglas-fir residue was removed by sulfite pretreatment to overcome the recalcitrance of lignocelluloses (SPORL). Sugar degradation to inhibitors was substantially reduced using a novel approach of "pH profiling" by delaying acid application in pretreatment, which facilitated the simultaneous enzymatic saccharification and fermentation of undetoxified whole slurry at a solid loading of 21%. Results: "pH profiling" reduced furan production by approximately 70% in using SPORL pretreating Douglas-fir forest residue (FS-10) comparing with the control run while without sacrificing enzymatic saccharification of the resultant substrate. pH profiling also reduced carbohydrate degradation. The improved carbohydrate yield in pretreated solids and reduced fermentation inhibitors with pH profiling resulted in a terminal ethanol titer of 48.9 +/- 1.4 g/L and yield of 297 +/- 9 L/tonne FS-10, which are substantially higher, i.e., by 27% in titer and by 38% in yield, than those of a control SPORL run without pH profiling. Conclusions: Economical and large-volume production of commodity biofuels requires the utilization of feedstocks with low value (therefore low cost) and sustainably producible in large quantities, such as forest residues. However, most existing pretreatment technologies cannot remove the strong recalcitrance of forest residues to produce practically fermentable high titer sugars. Here, we demonstrated a commercially scalable and efficient technology capable of removing the strong recalcitrant nature of forest residues using "pH profiling" together with "low temperature SPORL". The resultant pretreated whole slurry of a Douglas-fir forest residue using this technology can be easily processed at high solids of 21% without detoxification to achieve a high ethanol yield of 297 L/tonne at 48.9 g/L. C1 [Cheng, Jinlan] Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing, Jiangsu, Peoples R China. [Cheng, Jinlan; Leu, Shao-Yuan; Zhu, J. Y.; Gleisner, Rolland] USDA, Forest Prod Lab, Forest Serv, Madison, WI 53719 USA. [Leu, Shao-Yuan] Hong Kong Polytech Univ, Dept Civil & Environm Engn, Kowloon, Hong Kong, Peoples R China. RP Zhu, JY (reprint author), USDA, Forest Prod Lab, Forest Serv, Madison, WI 53719 USA. EM jzhu@fs.fed.us RI Leu, Shao-Yuan/C-7286-2017 OI Leu, Shao-Yuan/0000-0001-7262-1453 FU Agriculture and Food Research Initiative Competitive grant [2011-68005-30416]; USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA); Jiangsu Government Scholarship for Overseas Studies FX This work is supported by the Agriculture and Food Research Initiative Competitive grant (no. 2011-68005-30416), USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA). The authors thank Novozymes North America for providing the cellulase enzymes, Drs. Ronald Hector and Bruce Dien for providing the YRH400 strain, Fred Matt of USDA-FS-FPL for conducting chemical composition analysis, Diane Dietrich USDA-FS-FPL for assisting HPLC analyses, Drs. Xuejun Pan and Troy Runge of University of Wisconsin-Madison for allowing us access to their lab equipment, and Gevan Marrs of Weyerhaeuser Company and John Sessions of Oregon State University and the NARA feedstock team for providing the Douglas-fir forest residue. Jiangsu Government Scholarship for Overseas Studies provided partial financial support for the visiting appointment of Dr. Jinlan Cheng at the USDA Forest Service, Forest Products Laboratory (FPL), Madison, WI. NR 38 TC 7 Z9 8 U1 6 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD FEB 15 PY 2015 VL 8 AR 22 DI 10.1186/s13068-015-0205-3 PG 10 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA CC1MI UT WOS:000350104600001 PM 25709715 ER PT J AU Bowman, AS Krogwold, RA Price, T Davis, M Moeller, SJ AF Bowman, Andrew S. Krogwold, Roger A. Price, Todd Davis, Matt Moeller, Steven J. TI Investigating the introduction of porcine epidemic diarrhea virus into an Ohio swine operation SO BMC VETERINARY RESEARCH LA English DT Article DE Feed; PEDV; Swine ID UNITED-STATES; INFECTION; FEED AB Background: Porcine Epidemic Diarrhea virus (PEDV) is a highly transmissible coronavirus that causes a severe enteric disease that is particularly deadly for neonatal piglets. Since its introduction to the United States in 2013, PEDV has spread quickly across the country and has caused significant financial losses to pork producers. With no fully licensed vaccines currently available in the United States, prevention and control of PEDV disease is heavily reliant on biosecurity measures. Despite proven, effective biosecurity practices, multiple sites and production stages, within and across designated production flows in an Ohio swine operation broke with confirmed PEDV in January 2014, leading the producer and attending veterinarian to investigate the route of introduction. Case presentation: On January 12, 2014, several sows within a production flow were noted with signs of enteric illness. Within a few days, illness had spread to most of the sows in the facility and was confirmed by RT-PCR to be PEDV. Within a short time period, confirmed disease was present on multiple sites within and across breeding and post weaning production flows of the operation and mortality approached 100% in neonatal piglets. After an epidemiologic investigation, an outsourced, pelleted piglet diet was identified for assessment, and a bioassay, where naive piglets were fed the suspected feed pellets, was initiated to test the pellets for infectious PEDV. Conclusions: The epidemiological investigation provided strong evidence for contaminated feed as the source of the outbreak. In addition, feed pellets collected from unopened bags at the affected sites tested positive for PEDV using RT-PCR. However, the bioassay study was not able to show infectivity when feeding the suspected feed pellets to a small number of naive piglets. The results highlight the critical need for surveillance of feed and feed components to further define transmission avenues in an effort to limit the spread of PEDV throughout the U.S. swine industry. C1 [Bowman, Andrew S.] Ohio State Univ, Coll Vet Med, Columbus, OH 43210 USA. [Krogwold, Roger A.] USDA, APHIS, Vet Serv, Pickerington, OH 43147 USA. [Price, Todd] North Cent Vet Serv, Sycamore, OH 44882 USA. [Davis, Matt] Hord Livestock Co, Bucyrus, OH 44820 USA. [Moeller, Steven J.] Ohio State Univ, Coll Agr, Columbus, OH 43210 USA. RP Bowman, AS (reprint author), Ohio State Univ, Coll Vet Med, 1920 Coffey Rd, Columbus, OH 43210 USA. EM bowman.214@osu.edu RI Bowman, Andrew/B-4321-2012 OI Bowman, Andrew/0000-0002-0738-8453 FU National Pork Checkoff, PIC North America; U.S. Department of Agriculture; Ohio State University Open Access Fund FX We thank Jody Edwards for her expertise in scientific writing and editing and Tim Vojt for his medical illustration services. Funding was provided by the National Pork Checkoff, PIC North America and the U.S. Department of Agriculture. Partial funding for Open Access was provided by The Ohio State University Open Access Fund. Any mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The contents herein are solely the responsibility of the authors and do not necessarily represent the official views of the National Pork Board, PIC North America or the U.S. Department of Agriculture. NR 15 TC 12 Z9 13 U1 3 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD FEB 15 PY 2015 VL 11 AR 38 DI 10.1186/s12917-015-0348-2 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA CB8IK UT WOS:000349872000003 PM 25881144 ER PT J AU Ruffing, CM Daniels, MD Dwire, KA AF Ruffing, Claire M. Daniels, Melinda D. Dwire, Kathleen A. TI Disturbance legacies of historic tie-drives persistently alter geomorphology and large wood characteristics in headwater streams, southeast Wyoming SO GEOMORPHOLOGY LA English DT Article DE Instream wood; Channel disturbance; Wood removal; Mountain streams; Habitat; Riparian disturbance ID OLD-GROWTH FOREST; MOUNTAIN STREAMS; ORGANIC-MATTER; RIPARIAN VEGETATION; SEDIMENT STORAGE; RIVER CHANNELS; DEBRIS; RESTORATION; DYNAMICS; USA AB Instream wood is recognized as an integral component of stream morphology in forested areas. However, few studies have evaluated the legacy effects of historic wood removal activities and associated impacts on channel morphology, contemporary wood loading, and recruitment. This study investigates the role of historic tie-driving, a widespread channel disturbance legacy, in shaping present-day stream channel conditions in southern Wyoming. Geomorphic and riparian surveys were used to assess the extent of disturbance and degree of recovery within three sets of paired tie-driven and non-driven study reaches. Tie-driven streams were narrower, shallower, and had low cross-sectional roughness and higher width-to-depth ratios when compared to non-driven streams. Study reaches in first-order tie-driven streams were characterized by predominantly planebed morphologies and an extremely low abundance of wood compared to paired, non-driven reaches. Wood loads in second-order tie-driven reaches were similar to non-driven reaches, but overall wood distribution varied and was more likely to accumulate in jams. Existing wood loads in tie-driven reaches exhibited a narrower range of geomorphic functions and were less stable overall, although the relative state of decay was similar across all reaches. Basal area, stream power, and reach slope were identified as key mechanisms driving wood retention in the study reaches. The results of this study suggest that contemporary channel morphology and wood loads continue to reflect disturbance histories but have not yet been affected by other contemporary disturbances expected to influence wood loads such as bark beetle infestations. (C) 2014 Elsevier B.V. All rights reserved. C1 [Ruffing, Claire M.] Kansas State Univ, Dept Geog, Manhattan, KS 66506 USA. [Daniels, Melinda D.] Stroud Water Res Ctr, Avondale, PA 19311 USA. [Dwire, Kathleen A.] USDA Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Ruffing, CM (reprint author), Kansas State Univ, Dept Geog, 118 Seaton Hall, Manhattan, KS 66506 USA. EM cruffing@ksu.edu NR 84 TC 2 Z9 2 U1 0 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X EI 1872-695X J9 GEOMORPHOLOGY JI Geomorphology PD FEB 15 PY 2015 VL 231 BP 1 EP 14 DI 10.1016/j.geomorph.2014.10.029 PG 14 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA CA5OG UT WOS:000348957100001 ER PT J AU Kane, VR Lutz, JA Cansler, CA Povak, NA Churchill, DJ Smith, DF Kane, JT North, MP AF Kane, Van R. Lutz, James A. Cansler, C. Alina Povak, Nicholas A. Churchill, Derek J. Smith, Douglas F. Kane, Jonathan T. North, Malcolm P. TI Water balance and topography predict fire and forest structure patterns SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Mixed-severity fire; Forest structure; Random forests; RdNBR burn severity; LiDAR; Water balance ID YOSEMITE-NATIONAL-PARK; MIXED-CONIFER FORESTS; NORTHERN SIERRA-NEVADA; UNITED-STATES; SPATIAL-PATTERNS; BURN SEVERITY; LANDSCAPE DYNAMICS; PONDEROSA PINE; CASCADE RANGE; SURFACE FIRE AB Mountainous topography creates fine-scale environmental mosaics that vary in precipitation, temperature, insolation, and slope position. This mosaic in turn influences fuel accumulation and moisture and forest structure. We studied these the effects of varying environmental conditions across a 27,104 ha landscape within Yosemite National Park, California, USA, on the number of fires and burn severity (measured from Landsat data for 1984-2010) and on canopy cover at two heights (>2 m and 2-8 m) and dominant tree height (measured with airborne LiDAR data). We used site water balance (actual evapotranspiration and climatic water deficit) and topography (slope position, slope, and insolation) as environmental predictors. Random forest modeling showed that environmental conditions predicted substantial portions of the variations in fire and forest structure: e.g., 85-93% of the variation in whether a location did not burn, burned once, or burned twice; 64% of the variation in the burn severity; and 72% of the variation in canopy cover >2 m for unburned forests, 64% for once-burned forests, and 59% for twice-burned forests. Environmental conditions also predicted a substantial portion of forest structure following one and two fires, even though the post-fire forest structures were substantially different than pre-fire structures. This suggests a feedback mechanism in which local fire regimes and pre-fire forest structures are related to local environments, and their interaction produces post-fire structures also related to local environments. Among environmental predictors, water balance had the greatest explanatory power, followed by slope position, and then by slope and insolation. Managers could use our methods to help select reference areas that match environmental conditions, identify areas at risk for fires that endanger critical habitat or other resources, and identify climate analog areas to help anticipate and plan for climate change. (C) 2014 Elsevier B.V. All rights reserved. C1 [Kane, Van R.; Cansler, C. Alina; Churchill, Derek J.; Kane, Jonathan T.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Lutz, James A.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Povak, Nicholas A.] USDA, Forest Serv, Pacific Southwest Res Stn, Wenatchee Forestry Sci Lab, Wenatchee, WA 98801 USA. [Smith, Douglas F.] Yosemite Natl Pk, Yosemite, CA 95389 USA. [North, Malcolm P.] USDA, Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. RP Kane, VR (reprint author), Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. EM vkane@uw.edu OI Cansler, C. Alina/0000-0002-2155-4438 FU National Park Service; Fire and Aviation Management Branch; Fuels and Ecology Program [F8803100015]; U.S. Geological Survey Terrestrial, Freshwater, and Marine Environments Program; USDA Forest Service [14-JV-11272139-014, 13-CS-11052007-055] FX We thank Yosemite National Park for assistance with data management and field logistics. Funding for the LiDAR data collection and analysis was provided by the National Park Service, Fire and Aviation Management Branch, Fuels and Ecology Program (Interagency Agreement F8803100015), and the U.S. Geological Survey Terrestrial, Freshwater, and Marine Environments Program. We thank two anonymous reviewers who provided valuable comments on a previous version of this paper. R. Keala Hagmann, Sean Jeronimo, Marc Meyer, and Lauren S. Urgenson also provided valuable feedback. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US government. The USDA Forest Service supported theanalysis reported here through grants 14-JV-11272139-014 and 13-CS-11052007-055. NR 90 TC 19 Z9 20 U1 11 U2 78 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 15 PY 2015 VL 338 BP 1 EP 13 DI 10.1016/j.foreco.2014.10.038 PG 13 WC Forestry SC Forestry GA AZ5LN UT WOS:000348262600001 ER PT J AU Peterson, DW Dodson, EK Harrod, RJ AF Peterson, David W. Dodson, Erich K. Harrod, Richy J. TI Post-fire logging reduces surface woody fuels up to four decades following wildfire SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Ponderosa pine; Douglas-fir; Post-fire logging; Salvage logging; Fuel succession; Forest restoration ID PONDEROSA PINE ECOSYSTEMS; WESTERN UNITED-STATES; CASCADE MOUNTAINS; EASTERN CASCADES; CONIFER FORESTS; FIRE SEVERITY; SIERRA-NEVADA; DOUGLAS-FIR; DRY FORESTS; USA AB Severe wildfires create pulses of dead trees that influence future fuel loads, fire behavior, and fire effects as they decay and deposit surface woody fuels. Harvesting fire-killed trees may reduce future surface woody fuels and related fire hazards, but the magnitude and timing of post-fire logging effects on woody fuels have not been fully assessed. To address this issue, we sampled surface woody fuels within 255 coniferous forest stands that burned with high fire severity in 68 wildfires between 1970 and 2007 in eastern Washington and Oregon, USA. Sampling included 96 stands that were logged after wildfire and 159 stands that were not logged. Most forest stands sampled were dominated by ponderosa pine (Pinus ponderosa) or Douglas-fir (Pseudotsuga menziesii) prior to wildfire and historically supported low and mixed-severity fire regimes. In unlogged stands, woody fuel loads were low initially, but then increased and peaked 10-20 years following wildfire. In logged stands, small and medium diameter woody fuel loads peaked immediately after logging, whereas large diameter woody fuel loads peaked 10-20 years after wildfire. Relative to unlogged stands, post-fire logging initially increased surface woody fuel loads, increasing small diameter fuel loads by up to 2.1 Mg/ha during the first 5 years after fire and increasing medium diameter fuel loads by up to 5.8 Mg/ha during the first 7 years after fire. Logging subsequently reduced surface woody fuel loads, reducing large diameter fuel loads by up to 53 Mg/ha between 6 and 39 years after wildfire, reducing medium diameter fuel loads by up to 2.4 Mg/ha between 12 and 23 years after wildfire, and reducing small diameter fuel loads by up to 1.4 Mg/ha between 10 and 28 years after wildfire. Logging also reduced rotten, large diameter fuel loads by up to 24 Mg/ha between 20 and 39 years after wildfire. Our study suggests that post-fire logging can significantly reduce future surface woody fuel levels in forests regenerating following wildfires. The magnitude of woody fuel reduction depends, however, on the volume and sizes of wood removed, logging methods, post-logging fuel treatments, and the amount of coarse woody debris left on-site to support wildlife habitat, erosion control, and other competing management objectives. Published by Elsevier B.V. C1 [Peterson, David W.; Dodson, Erich K.] US Forest Serv, Pacific NW Res Stn, Wenatchee, WA 98801 USA. [Harrod, Richy J.] US Forest Serv, Okanogan Wenatchee Natl Forest, Wenatchee, WA 98801 USA. RP Peterson, DW (reprint author), US Forest Serv, Pacific NW Res Stn, 1133 N Western Ave, Wenatchee, WA 98801 USA. EM davepeterson@fs.fed.us; kyledodtnu@aol.com; rharrod@fs.fed.us FU U.S. Joint Fire Science Program [06-3-4-16]; U.S. National Fire Plan; U.S. Forest Service, Pacific Northwest Research Station FX This work was supported by the U.S. Joint Fire Science Program through Grant #06-3-4-16, the U.S. National Fire Plan, and the U.S. Forest Service, Pacific Northwest Research Station. We thank John Townsley for inspiring this study and assisting with the study design. We thank Peter Ohlson, Josephine West, Belinda Lo, Thomas McGinley, Matias Rudback, Chad Yenney, Sarah Eichler, and Rhys Logan for their help with stand selection and field data collection and James Dickinson for his help with Fig. 1. We also thank Tara Barrett and several anonymous reviewers for comments on earlier drafts of this paper. NR 47 TC 6 Z9 6 U1 6 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 15 PY 2015 VL 338 BP 84 EP 91 DI 10.1016/j.foreco.2014.11.016 PG 8 WC Forestry SC Forestry GA AZ5LN UT WOS:000348262600008 ER PT J AU Desai, AR Xu, K Tian, H Weishampel, P Thom, J Baumann, D Andrews, AE Cook, BD King, JY Kolka, R AF Desai, Ankur R. Xu, Ke Tian, Hanqin Weishampel, Peter Thom, Jonathan Baumann, Dan Andrews, Arlyn E. Cook, Bruce D. King, Jennifer Y. Kolka, Randall TI Landscape-level terrestrial methane flux observed from a very tall tower SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Methane; Eddy covariance; Regional flux; Land-atmosphere ID CARBON-DIOXIDE FLUXES; PROCESS-BASED MODEL; EDDY-COVARIANCE; NATURAL WETLANDS; CLIMATE-CHANGE; TUNDRA ECOSYSTEM; INTEGRATED MODEL; UNITED-STATES; NORTH-AMERICA; UPPER MIDWEST AB Simulating the magnitude and variability of terrestrial methane sources and sinks poses a challenge to ecosystem models because the biophysical and biogeochemical processes that lead to methane emissions from terrestrial and freshwater ecosystems are, by their nature, episodic and spatially disjunct. As a consequence, model predictions of regional methane emissions based on field campaigns from short eddy covariance towers or static chambers have large uncertainties, because measurements focused on a particular known source of methane emission will be biased compared to regional estimates with regards to magnitude, spatial scale, or frequency of these emissions. Given the relatively large importance of predicting future terrestrial methane fluxes for constraining future atmospheric methane growth rates, a clear need exists to reduce spatiotemporal uncertainties. In 2010, an Ameriflux tower (US-PFa) near Park Falls, WI, USA, was instrumented with closed-path methane flux measurements at 122 m above ground in a mixed wetland-upland landscape representative of the Great Lakes region. Two years of flux observations revealed an average annual methane (CH4) efflux of 785 +/- 75 mg C-CH4 m(-2) yr(-1), compared to a mean CO2 sink of -80g C-CO2 m(-2) yr(-1), a ratio of 1% in magnitude on a mole basis. Interannual variability in methane flux was 30% of the mean flux and driven by suppression of methane emissions during dry conditions in late summer 2012. Though relatively small, the magnitude of the methane source from the very tall tower measurements was mostly within the range previously measured using static chambers at nearby wetlands, but larger than a simple scaling of those fluxes to the tower footprint. Seasonal patterns in methane fluxes were similar to those simulated in the Dynamic Land Ecosystem Model (DLEM), but magnitude depends on model parameterization and input data, especially regarding wetland extent. The model was unable to simulate short-term (sub-weekly) variability. Temperature was found to be a stronger driver of regional CH4 flux than moisture availability or net ecosystem production at the daily to monthly scale. Taken together, these results emphasize the multi-timescale dependence of drivers of regional methane flux and the importance of long, continuous time series for their characterization. (C) 2014 Elsevier B.V. All rights reserved. C1 [Desai, Ankur R.; Xu, Ke; Thom, Jonathan] Univ Wisconsin, Ctr Climat Res, Madison, WI 53706 USA. [Tian, Hanqin] Auburn Univ, Int Ctr Climate & Global Change Res, Auburn, AL 36849 USA. [Weishampel, Peter] Natl Ecol Observ Network Inc, Great Lakes Domain, Land O Lakes, WI USA. [Baumann, Dan] US Geol Survey, Wisconsin Water Sci Ctr, Rhinelander, WI USA. [Andrews, Arlyn E.] NOAA, Earth Syst Res Lab, Boulder, CO USA. [Cook, Bruce D.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [King, Jennifer Y.] Univ Calif Santa Barbara, Dept Geog, Santa Barbara, CA 93106 USA. [Kolka, Randall] US Forest Serv, USDA, No Res Stn, Grand Rapids, MI USA. RP Desai, AR (reprint author), Univ Wisconsin, Ctr Climat Res, Madison, WI 53706 USA. EM desai@aos.wisc.edu RI Andrews, Arlyn/K-3427-2012; Desai, Ankur/A-5899-2008; King, Jennifer Y./I-5986-2015; Tian, Hanqin/A-6484-2012 OI Desai, Ankur/0000-0002-5226-6041; King, Jennifer Y./0000-0003-3433-5952; Tian, Hanqin/0000-0002-1806-4091 FU National Science Foundation (NSF) biology directorate [DEB-0845166, DBI-1062204]; NASA NACP Project [NNG05GD51G]; USDA Forest Service Northern Global Change program; NOAA FX This work was supported by National Science Foundation (NSF) biology directorate grants DEB-0845166 and DBI-1062204. We also acknowledge the contributions of R. Strand and J. Ayers at State of Wisconsin Educational Communications Board, K. Davis at The Pennsylvania State University, and P. Bolstad at the University of Minnesota. Static chamber measurements were supported by NASA NACP Project # NNG05GD51G and the USDA Forest Service Northern Global Change program. Jonathan Kofler and Jonathan Williams were funded by NOAA to provide site and CO2 and CH4 profile instrument support. This project contributes to the North American Carbon Program. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 114 TC 6 Z9 6 U1 6 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD FEB 15 PY 2015 VL 201 BP 61 EP 75 DI 10.1016/j.agrformet.2014.10.017 PG 15 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AY9IN UT WOS:000347863900007 ER PT J AU Cheng, SJ Bohrer, G Steiner, AL Hollinger, DY Suyker, A Phillips, RP Nadelhoffer, KJ AF Cheng, Susan J. Bohrer, Gil Steiner, Allison L. Hollinger, David Y. Suyker, Andrew Phillips, Richard P. Nadelhoffer, Knute J. TI Variations in the influence of diffuse light on gross primary productivity in temperate ecosystems SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Net ecosystem exchange; Diffuse PAR; Carbon cycling; Land-atmosphere interactions ID CARBON-DIOXIDE EXCHANGE; AMERICAN DECIDUOUS FORESTS; USE EFFICIENCY; SKY CONDITIONS; CANOPY; RADIATION; PHOTOSYNTHESIS; STORAGE; IMPACT; LAND AB The carbon storage potential of terrestrial ecosystems depends in part on how atmospheric conditions influence the type and amount of surface radiation available for photosynthesis. Diffuse light, resulting from interactions between incident solar radiation and atmospheric aerosols and clouds, has been postulated to increase carbon uptake in terrestrial ecosystems. However, the magnitude of the diffuse light effect is unclear because existing studies use different methods to derive above-canopy diffuse light conditions. We used site-based, above-canopy measurements of diffuse light and gross primary productivity (GPP) from 10 temperate ecosystems (including mixed conifer forests, deciduous broadleaf forests, and croplands) to quantify the GPP variation explained by diffuse photosynthetically active radiation (PAR) and to calculate increases in GPP as a function of diffuse light. Our analyses show that diffuse PAR explained up to 41% of variation in GPP in croplands and up to 17% in forests, independent of direct light levels. Carbon enhancement rates in response to diffuse PAR (calculated after accounting for vapor pressure deficit and air temperature) were also higher in croplands (0.011-0.050 mu mol CO2 per kmol photons of diffuse PAR) than in forests (0.003-0.018 mu mol CO2 per kmol photons of diffuse PAR). The amount of variation in GPP and carbon enhancement rate both differed with solar zenith angle and across sites for the same plant functional type. At crop sites, diffuse PAR had the strongest influence and the largest carbon enhancement rate during early mornings and late afternoons when zenith angles were large, with greater enhancement in the afternoons. In forests, diffuse PAR had the strongest influence at small zenith angles, but the largest carbon enhancement rate at large zenith angles, with a trend in ecosystem-specific responses. These results highlight the influence of zenith angle and the role of plant community composition in modifying diffuse light enhancement in terrestrial ecosystems, which will be important in scaling this effect from individual sites to the globe. (C) 2014 Elsevier B.V. All rights reserved. C1 [Cheng, Susan J.; Nadelhoffer, Knute J.] Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. [Bohrer, Gil] Ohio State Univ, Dept Civil Environm & Geodet Engn, Columbus, OH 43210 USA. [Steiner, Allison L.] Univ Michigan, Dept Atmospher Ocean & Space Sci, Ann Arbor, MI 48109 USA. [Hollinger, David Y.] US Forest Serv, No Res Stn, USDA, Durham, NH 03824 USA. [Suyker, Andrew] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Phillips, Richard P.] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA. RP Cheng, SJ (reprint author), Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA. EM chengs@umich.edu RI Hollinger, David/G-7185-2012; Steiner, Allison/F-4942-2011; OI Bohrer, Gil/0000-0002-9209-9540 FU National Science Foundation [DEB-0911461]; U.S. Department of Energy's (DOE) Office of Science Biological and Environmental Research (BER) [DESC0006708]; AmeriFlux National Core Flux Site award through Lawrence Berkeley National Laboratory [7096915] FX We thank the FLUXNET community for their dedicated efforts in collecting and providing quality-controlled eddy covariance data for ecosystem research. We also thank the University of Michigan Center for Statistical Consultation and Research (Dr. Corey Powell and Dr. Kerby Shedden) for input on statistical techniques, Dr. Christoph Vogel for providing updated PAR data for UMBS, and Alex Fotis for early discussions and data organization for this paper. Support for SJC was provided in part by the University of Michigan Graham Sustainability Institute. Funding for data collection at UMBS was provided by the National Science Foundation grant DEB-0911461, the U.S. Department of Energy's (DOE) Office of Science Biological and Environmental Research (BER) project DESC0006708 and AmeriFlux National Core Flux Site award through Lawrence Berkeley National Laboratory contract #7096915, and National Oceanic and Atmospheric Administration Grant NA110AR4310190. Research at the Howland Forest is supported by the DOE's Office of Science BER. The Mead US-Ne1, US-Ne2, and USNe3 AmeriFlux sites were supported by the DOE Office of Science (BER; Grant Nos. DE-FG03-00ER62996, DE-FG02-03ER63639, and DE-EE0003149), DOE-EPSCoR (Grant No. DE-FG02-00ER45827), and NASA NACP (Grant No. NNX08AI75G). The Morgan Monroe team thanks the Indiana Department of Natural Resources for supporting and hosting the UM-MMS AmeriFlux site, and the U.S. DOE for funding operations through the Terrestrial Ecosystem Science program and the AmeriFlux Management Project. NR 56 TC 11 Z9 11 U1 11 U2 71 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD FEB 15 PY 2015 VL 201 BP 98 EP 110 DI 10.1016/j.agrformet.2014.11.002 PG 13 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AY9IN UT WOS:000347863900010 ER PT J AU Collick, AS Fuka, DR Kleinman, PJA Buda, AR Weld, JL White, MJ Veith, TL Bryant, RB Bolster, CH Easton, ZM AF Collick, Amy S. Fuka, Daniel R. Kleinman, Peter J. A. Buda, Anthony R. Weld, Jennifer L. White, Mike J. Veith, Tamie L. Bryant, Ray B. Bolster, Carl H. Easton, Zachary M. TI Predicting phosphorus dynamics in complex terrains using a variable source area hydrology model SO HYDROLOGICAL PROCESSES LA English DT Article DE curve number (CN); watershed model; non-point source pollution; saturation excess; variable source area (VSA); water quality ID EFFECTS ASSESSMENT PROJECT; NONPOINT-SOURCE POLLUTION; 2 AGRICULTURAL HILLSLOPES; WATER ASSESSMENT-TOOL; SWAT MODEL; DISSOLVED PHOSPHORUS; CENTRAL PENNSYLVANIA; RUNOFF GENERATION; SURFACE RUNOFF; SOIL AB Phosphorus (P) loss from agricultural watersheds has long been a critical water quality problem, the control of which has been the focus of considerable research and investment. Preventing P loss depends on accurately representing the hydrological and chemical processes governing P mobilization and transport. The Soil and Water Assessment Tool (SWAT) is a watershed model commonly used to predict run-off and non-point source pollution transport. SWAT simulates run-off employing either the curve number (CN) or the Green and Ampt methods, both assume infiltration-excess run-off, although shallow soils underlain by a restricting layer commonly generate saturation-excess run-off from variable source areas (VSA). In this study, we compared traditional SWAT with a re-conceptualized version, SWAT-VSA, that represents VSA hydrology, in a complex agricultural watershed in east central Pennsylvania. The objectives of this research were to provide further evidence of SWAT-VSA's integrated and distributed predictive capabilities against measured surface run-off and stream P loads and to highlight the model's ability to drive sub-field management of P. Thus, we relied on a detailed field management database to parameterize the models. SWAT and SWAT-VSA predicted discharge similarly well (daily Nash-Sutcliffe efficiencies of 0.61 and 0.66, respectively), but SWAT-VSA outperformed SWAT in predicting P export from the watershed. SWAT estimated lower P loss (0.0-0.25kgha(-1)) from agricultural fields than SWAT-VSA (0.0-1.0+ kgha(-1)), which also identified critical source areas - those areas generating large run-off and P losses at the sub-field level. These results support the use of SWAT-VSA in predicting watershed-scale P losses and identifying critical source areas of P loss in landscapes with VSA hydrology. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Collick, Amy S.; Kleinman, Peter J. A.; Buda, Anthony R.; Veith, Tamie L.; Bryant, Ray B.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Fuka, Daniel R.; Easton, Zachary M.] Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24060 USA. [Weld, Jennifer L.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [White, Mike J.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Bolster, Carl H.] USDA ARS, Anim Waste Management Res Unit, Bowling Green, KY 42104 USA. RP Easton, ZM (reprint author), Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24060 USA. EM zeaston@vt.edu RI Veith, Tamie/H-4911-2011 NR 64 TC 13 Z9 13 U1 4 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD FEB 15 PY 2015 VL 29 IS 4 BP 588 EP 601 DI 10.1002/hyp.10178 PG 14 WC Water Resources SC Water Resources GA AY9DD UT WOS:000347848900010 ER PT J AU Pepin, KM Leach, CB Marques-Toledo, C Laass, KH Paixao, KS Luis, AD Hayman, DTS Johnson, NG Buhnerkempe, MG Carver, S Grear, DA Tsao, K Eiras, AE Webb, CT AF Pepin, Kim M. Leach, Clint B. Marques-Toledo, Cecilia Laass, Karla H. Paixao, Kelly S. Luis, Angela D. Hayman, David T. S. Johnson, Nels G. Buhnerkempe, Michael G. Carver, Scott Grear, Daniel A. Tsao, Kimberly Eiras, Alvaro E. Webb, Colleen T. TI Utility of mosquito surveillance data for spatial prioritization of vector control against dengue viruses in three Brazilian cities SO PARASITES & VECTORS LA English DT Article DE Vector control; Dengue; Surveillance; Vector density; Mosquito-human interactions; Gravity model; INLA ID RIO-DE-JANEIRO; AEDES-AEGYPTI; HUMAN MOVEMENT; DIPTERA-CULICIDAE; THAI VILLAGES; TRANSMISSION; DYNAMICS; TRAPS; MEASLES; ADULT AB Background: Vector control remains the primary defense against dengue fever. Its success relies on the assumption that vector density is related to disease transmission. Two operational issues include the amount by which mosquito density should be reduced to minimize transmission and the spatio-temporal allotment of resources needed to reduce mosquito density in a cost-effective manner. Recently, a novel technology, MI-Dengue, was implemented city-wide in several Brazilian cities to provide real-time mosquito surveillance data for spatial prioritization of vector control resources. We sought to understand the role of city-wide mosquito density data in predicting disease incidence in order to provide guidance for prioritization of vector control work. Methods: We used hierarchical Bayesian regression modeling to examine the role of city-wide vector surveillance data in predicting human cases of dengue fever in space and time. We used four years of weekly surveillance data from Vitoria city, Brazil, to identify the best model structure. We tested effects of vector density, lagged case data and spatial connectivity. We investigated the generality of the best model using an additional year of data from Vitoria and two years of data from other Brazilian cities: Governador Valadares and Sete Lagoas. Results: We found that city-wide, neighborhood-level averages of household vector density were a poor predictor of dengue-fever cases in the absence of accounting for interactions with human cases. Effects of city-wide spatial patterns were stronger than within-neighborhood or nearest-neighborhood effects. Readily available proxies of spatial relationships between human cases, such as economic status, population density or between-neighborhood roadway distance, did not explain spatial patterns in cases better than unweighted global effects. Conclusions: For spatial prioritization of vector controls, city-wide spatial effects should be given more weight than within-neighborhood or nearest-neighborhood connections, in order to minimize city-wide cases of dengue fever. More research is needed to determine which data could best inform city-wide connectivity. Once these data become available, MI-dengue may be even more effective if vector control is spatially prioritized by considering city-wide connectivity between cases together with information on the location of mosquito density and infected mosquitos. C1 [Pepin, Kim M.; Luis, Angela D.; Webb, Colleen T.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. [Pepin, Kim M.] USDA, Natl Wildlife Res Ctr, Wildlife Serv, Anim & Plant Hlth Inspect Serv, Ft Collins, CO 80521 USA. [Pepin, Kim M.; Leach, Clint B.; Luis, Angela D.; Hayman, David T. S.; Johnson, Nels G.; Buhnerkempe, Michael G.; Carver, Scott; Grear, Daniel A.; Tsao, Kimberly; Webb, Colleen T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Marques-Toledo, Cecilia] Ecovec SA, Belo Horizonte, MG, Brazil. [Laass, Karla H.; Paixao, Kelly S.; Eiras, Alvaro E.] Univ Fed Minas Gerais, Dept Parasitol, Belo Horizonte, MG, Brazil. [Hayman, David T. S.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Carver, Scott] Univ Tasmania, Sch Biol Sci, Hobart, Tas 7000, Australia. RP Pepin, KM (reprint author), NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM Kim.M.Pepin@aphis.usda.gov; alvaro@icb.ufmg.br RI Carver, Scott/J-7654-2014 OI Carver, Scott/0000-0002-3579-7588 FU RAPIDD program of the Science and Technology Directorate; U.S. Department of Homeland Security; Fogarty International Center, NIH; USDA-APHIS-WS; CNPq [550131/2010-8, 404211/2012-7]; ICNT-Dengue; David H Smith postdoctoral fellowship; University of Tasmania Research Enhancement Grant Scheme [C 20897]; NSF Graduate Research Fellowship Program [DGE-1321845] FX Thanks to the dengue control program managers in the cities of Vitoria, GV and SL, especially to Andre Capezzuto, Jose Batista and Maria Jose Lanza, respectively, for help in obtaining the data for analyses. KMP, ADL, DTSH and CTW were funded by the RAPIDD program of the Science and Technology Directorate, U.S. Department of Homeland Security, and the Fogarty International Center, NIH. KMP was also funded by USDA-APHIS-WS during the latter part of the study. AEE, CMT, KHL and KSP were funded by CNPq (Pronex-Dengue - grants # 550131/2010-8 and Doenaas Neglenciadas # 404211/2012-7) and ICNT-Dengue. DTSH acknowledges the David H Smith postdoctoral fellowship for funding. SC acknowledges funding from the University of Tasmania Research Enhancement Grant Scheme (grant # C 20897). CBL was supported by the NSF Graduate Research Fellowship Program under grant DGE-1321845. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of USDA-APHIS-VS, USDA-APHIS-WS, DHS or NIH. NR 36 TC 2 Z9 2 U1 1 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 15 PY 2015 VL 8 AR 98 DI 10.1186/s13071-015-0659 PG 15 WC Parasitology SC Parasitology GA DD3RT UT WOS:000369841000001 PM 25889533 ER PT J AU Winkler-Moser, JK Hwang, HS Bakota, EL Palmquist, DA AF Winkler-Moser, Jill K. Hwang, Hong-Sik Bakota, Erica L. Palmquist, Debra A. TI Synthesis of steryl ferulates with various sterol structures and comparison of their antioxidant activity SO FOOD CHEMISTRY LA English DT Article DE Steryl ferulates; Phytosterols; Soybean oil; Frying; Antioxidant activity; Antipolymerisation activity ID GAMMA-ORYZANOL; OXIDATIVE STABILITY; SOYBEAN OIL; FERULIC ACID; CORN; POLYMERIZATION; ESTERS; RICE; BRAN; PHYTOSTEROLS AB Steryl ferulates synthesised from commercial sterols as well as commercial oryzanol were used to better understand how structural features affect antioxidant activity in vitro by the ABTS radical decolorization assay, by oxidative stability index (OSI) of soybean oil, and by analysis of antioxidant activity during frying. Steryl ferulates inhibited the ABTS(+) radical by 6.5-56.6%, depending on their concentration, but were less effective, especially at lower concentrations, than ferulic acid. Ferulic acid and steryl ferulates had either no effect, or lowered the OSI of soybean oil by up to 25%, depending on the concentration. In their evaluation as frying oil antioxidants, steryl ferulates with a saturated sterol group had the best antioxidant activity, followed by sterols with one double bond in the C5 position. The results indicate that a dimethyl group at C4 as well as a C9,C19 cyclopropane group, as found in oryzanol, negatively affects antioxidant activity in frying oils. Published by Elsevier Ltd. C1 [Winkler-Moser, Jill K.; Hwang, Hong-Sik; Bakota, Erica L.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Palmquist, Debra A.] ARS, USDA, Midwest Area, Peoria, IL 61604 USA. RP Winkler-Moser, JK (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Jill.Moser@ars.usda.gov NR 30 TC 5 Z9 6 U1 5 U2 112 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD FEB 15 PY 2015 VL 169 BP 92 EP 101 DI 10.1016/j.foodchem.2014.07.119 PG 10 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AR1IV UT WOS:000343339800014 PM 25236203 ER PT J AU Wang, QG Cao, Y Zhou, LL Jiang, CZ Feng, YY Wei, SC AF Wang, Qingguo Cao, Yu Zhou, Lili Jiang, Cai-Zhong Feng, Yanyan Wei, Shaochong TI Effects of postharvest curing treatment on flesh colour and phenolic metabolism in fresh-cut potato products SO FOOD CHEMISTRY LA English DT Article DE Fresh-cut potato; Browning; Curing; Phenolic metabolism ID DELAYING FUNGICIDE TREATMENT; POLYPHENOL OXIDASE; CONTROLLED ATMOSPHERES; BROWNING INHIBITORS; HARVESTED LETTUCE; RESPIRATION RATE; QUALITY; STORAGE; ETHYLENE; ENZYMES AB The flesh colour and phenolic metabolism in potato tuber during curing and after cut were investigated. Result indicated that postharvest curing not only changed phenolic metabolism during curing, but also improved fresh-cut colour for 12 days after fresh cut. Significantly lower PAL and higher phenolic content and PPO activities during curing treatment and fresh-cut potatoes were detected compared to the control, which lead to the lower browning in the slices from curing treated potatoes. HPLC analysis revealed that amounts of total phenolics, chlorogenic acid, gallic acid and protocatechuic acid were induced by curing and highly accumulated in the curing treated potatoes. Our results demonstrated that phenolic metabolism played an important role in the control of browning of fresh cut potato after curing. (C) 2014 The Authors. Published by Elsevier Ltd. C1 [Wang, Qingguo; Cao, Yu; Zhou, Lili; Feng, Yanyan] Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China. [Jiang, Cai-Zhong] USDA ARS, Davis, CA 95616 USA. [Wei, Shaochong] Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China. RP Wang, QG (reprint author), Shandong Agr Univ, Coll Food Sci & Engn, Room 304,Bldg 9,61 Daizong St, Tai An 271018, Shandong, Peoples R China. EM wqgyyy@126.com FU Natural Science Foundation of Shandong Province, China [ZR2013CM012]; Potato Industry Innovation Team for Modern Agricultural Industry Technology System, Shandong Province [SDAIT-10-011-11] FX This research work was partially supported by a Grant from Natural Science Foundation of Shandong Province, China, #ZR2013CM012 and a Grant from Potato Industry Innovation Team for Modern Agricultural Industry Technology System, Shandong Province, #SDAIT-10-011-11. NR 36 TC 4 Z9 4 U1 15 U2 115 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD FEB 15 PY 2015 VL 169 BP 246 EP 254 DI 10.1016/j.foodchem.2014.08.011 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AR1IV UT WOS:000343339800034 PM 25236223 ER PT J AU Martynov, AG Elpidina, EN Perkin, L Oppert, B AF Martynov, Alexander G. Elpidina, Elena N. Perkin, Lindsey Oppert, Brenda TI Functional analysis of C1 family cysteine peptidases in the larval gut of Tenebrio molitor and Tribolium castaneum SO BMC GENOMICS LA English DT Article DE High throughput sequencing; Cysteine peptidases; Cathepsin L; Cathepsin B; Peptidase homologs; Tenebrio molitor; Tribolium castaneum ID CATHEPSIN-L-LIKE; MULTIPLE SEQUENCE ALIGNMENT; L-LIKE PROTEINASES; MOLECULAR CHARACTERIZATION; DIGESTIVE PROTEINASES; MIDGUT; COLEOPTERA; BEETLE; ORGANIZATION; TRANSCRIPTS AB Background: Larvae of the tenebrionids Tenebrio molitor and Tribolium castaneum have highly compartmentalized guts, with primarily cysteine peptidases in the acidic anterior midgut that contribute to the early stages of protein digestion. Results: High throughput sequencing was used to quantify and characterize transcripts encoding cysteine peptidases from the C1 papain family in the gut of tenebrionid larvae. For T. castaneum, 25 genes and one questionable pseudogene encoding cysteine peptidases were identified, including 11 cathepsin L or L-like, 11 cathepsin B or B-like, and one each F, K, and O. The majority of transcript expression was from two cathepsin L genes on chromosome 10 (LOC659441 and LOC659502). For cathepsin B, the major expression was from genes on chromosome 3 (LOC663145 and LOC663117). Some transcripts were expressed at lower levels or not at all in the larval gut, including cathepsins F, K, and O. For T. molitor, there were 29 predicted cysteine peptidase genes, including 14 cathepsin L or L-like, 13 cathepsin B or B-like, and one each cathepsin O and F. One cathepsin L and one cathepsin B were also highly expressed, orthologous to those in T. castaneum. Peptidases lacking conservation in active site residues were identified in both insects, and sequence analysis of orthologs indicated that changes in these residues occurred prior to evolutionary divergence. Sequences from both insects have a high degree of variability in the substrate binding regions, consistent with the ability of these enzymes to degrade a variety of cereal seed storage proteins and inhibitors. Predicted cathepsin B peptidases from both insects included some with a shortened occluding loop without active site residues in the middle, apparently lacking exopeptidase activity and unique to tenebrionid insects. Docking of specific substrates with models of T. molitor cysteine peptidases indicated that some insect cathepsins B and L bind substrates with affinities similar to human cathepsin L, while others do not and have presumably different substrate specificity. Conclusions: These studies have refined our model of protein digestion in the larval gut of tenebrionid insects, and suggest genes that may be targeted by inhibitors or RNA interference for the control of cereal pests in storage areas. C1 [Martynov, Alexander G.] Skolkovo Inst Sci & Technol, Dept Biomed Sci & Technol, Skolkovo 143025, Russia. [Martynov, Alexander G.] Moscow MV Lomonosov State Univ, Fac Bioengn & Bioinformat, Moscow 119991, Russia. [Martynov, Alexander G.; Elpidina, Elena N.] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Moscow 119991, Russia. [Perkin, Lindsey; Oppert, Brenda] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Oppert, B (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. EM bso@ksu.edu OI Martynov, Alexander/0000-0003-1845-5209 FU ISTC [3455]; Russian Foundation for Basic Research [12-04-01562-a, 14-04-91167-NSFC_a, 15-04-08689-a] FX The research was performed with the support of ISTC grant #3455 and the Russian Foundation for Basic Research (grants # 12-04-01562-a, # 14-04-91167-NSFC_a, and 15-04-08689-a). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 55 TC 5 Z9 5 U1 0 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD FEB 14 PY 2015 VL 16 AR 75 DI 10.1186/s12864-015-1306-x PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CC2GC UT WOS:000350162200001 PM 25757364 ER PT J AU Edwards, JV Prevost, NT French, AD Concha, M Condon, BD AF Edwards, J. Vincent Prevost, Nicolette T. French, Alfred D. Concha, Monica Condon, Brian D. TI Kinetic and structural analysis of fluorescent peptides on cotton cellulose nanocrystals as elastase sensors SO CARBOHYDRATE POLYMERS LA English DT Article; Proceedings Paper CT 3rd EPNOE International Polysaccharide Conference CY OCT 21-24, 2013 CL EPNOE, Nice, FRANCE SP Cellulose & Renewable Mat Div, ACS, Bionavis, Cargill, Cellulose Springer, Conseil Reg Provence Alpes Cote D Azur, Elsevier Carbohydrate Polymers, Journal Appl Polymer Sci, Wyatt Technol HO EPNOE DE Biosensor; Human neutrophil elastase; Cotton/cellulose; Nanocrystals ID PORCINE PANCREATIC ELASTASE; HUMAN-LEUKOCYTE ELASTASE; NEUTROPHIL ELASTASE; CATHEPSIN-G; CRYSTAL-STRUCTURES; SURFACE OXIDATION; X-RAY; MICRODIALYSIS; PROTEINASE-3; TECHNOLOGY AB Human neutrophil elastase (HNE) and porcine pancreatic elastase (PPE) are serine proteases with destructive proteolytic activity. Because of this activity, there is considerable interest in elastase sensors. Herein we report the synthesis, characterization, and kinetic profiles of tri- and tetrapeptide substrates of elastase as glycine-esterified fluorescent analogs of cotton cellulose nanocrystals (CCN). The degree of substitution of peptide incorporated in CCN was 3-4 peptides per 100 anhydroglucose units. Glycine and peptide-cellulose-nanocrystals revealed crystallinity indices of 79 and 76%, respectively, and a crystallite size of 58.5 angstrom. A crystallite model of the peptide-cellulose conjugate is shown. The tripeptide conjugate of CCN demonstrated five-fold greater efficiency in HNE than the tripeptide in solution judged by its k(cat)/K-m of 33,515. The sensor limits of detection at 2 mg of the tri- and tetrapeptide CCN conjugates over a 10 min reaction time course were 0.03 U/mL PPE and 0.05 U/mL HNE, respectively. Published by Elsevier Ltd. C1 [Edwards, J. Vincent; Prevost, Nicolette T.; French, Alfred D.; Concha, Monica; Condon, Brian D.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. EM vince.edwards@ars.usda.gov NR 45 TC 4 Z9 4 U1 6 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD FEB 13 PY 2015 VL 116 BP 278 EP 285 DI 10.1016/j.carbpol.2014.04.067 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA AT3YJ UT WOS:000344871200037 PM 25458301 ER PT J AU Zhang, Y Ding, ZH Ma, FF Chauhan, RD Allen, DK Brutnell, TP Wang, WQ Peng, M Li, PH AF Zhang, Yang Ding, Zehong Ma, Fangfang Chauhan, Raj Deepika Allen, Doug K. Brutnell, Thomas P. Wang, Wenquan Peng, Ming Li, Pinghua TI Transcriptional response to petiole heat girdling in cassava SO SCIENTIFIC REPORTS LA English DT Article ID MANIHOT-ESCULENTA CRANTZ; PHASEOLUS-VULGARIS; PHLOEM TRANSLOCATION; EXPRESSION ANALYSIS; GENE-EXPRESSION; CYTOCHROME B5; RNA-SEQ; PHOTOSYNTHESIS; LEAVES; STARCH AB To examine the interactions of starch and sugar metabolism on photosynthesis in cassava, a heat-girdling treatment was applied to petioles of cassava leaves at the end of the light cycle to inhibit starch remobilization during the night. The inhibition of starch remobilization caused significant starch accumulation at the beginning of the light cycle, inhibited photosynthesis, and affected intracellular sugar levels. RNA-seq analysis of heat-treated and control plants revealed significantly decreased expression of genes related to photosynthesis, as well as N-metabolism and chlorophyll biosynthesis. However, expression of genes encoding TCA cycle enzymes and mitochondria electron transport components, and flavonoid biosynthetic pathway enzymes were induced. These studies reveal a dynamic transcriptional response to perturbation of sink demand in a single leaf, and provide useful information for understanding the regulations of cassava under sink or source limitation. C1 [Zhang, Yang; Ding, Zehong; Wang, Wenquan; Peng, Ming; Li, Pinghua] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China. [Ma, Fangfang; Chauhan, Raj Deepika; Allen, Doug K.; Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Allen, Doug K.] Agr Res Serv, USDA, Plant Genet Res Unit, St Louis, MO 63132 USA. [Li, Pinghua] Shandong Agr Univ, Coll Agron Sci, Tai An 271018, Shandong, Peoples R China. RP Li, PH (reprint author), Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China. EM Pinghuali@sdau.edu.cn RI Brutnell, Thomas/M-2840-2013; Allen, Doug/M-2836-2013 OI Brutnell, Thomas/0000-0002-3581-8211; Allen, Doug/0000-0001-8599-8946 FU National Basic Research Program of China (973 Program) [2010CB126601]; Technology Foundation for Selected Overseas Chinese Scholar; NSF [EF-1105249]; USDA-ARS FX We would like to thank Dr. James Schnable for his suggestions in data processing, Ying Shao for her assistance with RNA-Seq library construction and Dr. Jenny Xiang for RNA-sequencing, Dr. Wen Zhou and Peng Liu of their data analysis that not included in the manuscript, and Dr. Nigel Taylor for supporting on tissue-cultured cassava plants. We also thank the National Basic Research Program of China (973 Program, 2010CB126601) and Technology Foundation for Selected Overseas Chinese Scholar funding to P. Li, NSF (EF-1105249) and the USDA-ARS. Any product or trademark mentioned here does not imply a warranty, guarantee, or endorsement by the authors or their affiliations over other suitable products. Author contributions. NR 44 TC 5 Z9 5 U1 1 U2 20 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 12 PY 2015 VL 5 AR 8414 DI 10.1038/srep08414 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA9LR UT WOS:000349245600013 PM 25672661 ER PT J AU de Lima, RSN Figueiredo, FAMMD Martins, AO de Deus, BCD Ferraz, TM Gomes, MDD de Sousa, EF Glenn, DM Campostrini, E AF Nunes de Lima, Roberta Samara Moura de Assis Figueiredo, Fabio Afonso Mazzei Martins, Amanda Oliveira da Silva de Deus, Bruna Correa Ferraz, Tiago Massi de Assis Gomes, Mara de Menezes de Sousa, Elias Fernandes Glenn, David Michael Campostrini, Eliemar TI Partial rootzone drying (PRD) and regulated deficit irrigation (RDI) effects on stomatal conductance, growth, photosynthetic capacity, and water-use efficiency of papaya SO SCIENTIA HORTICULTURAE LA English DT Article DE Carica papaya; Soil moisture; Root ID ZONE DRIP IRRIGATION; LEAF GAS-EXCHANGE; CHLOROPHYLL FLUORESCENCE; PHYSIOLOGICAL-RESPONSES; HORMONAL CHANGES; YIELD RESPONSE; FRUIT-QUALITY; APPLE-TREES; PINK LADY; DROUGHT AB Papaya (Carica papaya, L.) is an important economic crop in tropical and subtropical countries. It is a giant herbaceous species; maintaining adequate tissue turgidity and water availability is necessary to maintain the rigidity of the stem as well as increase productivity. Water-saving irrigation techniques for papaya will be needed in the future due to the possibility of water shortages related to climate change. The morphological and physiological responses of 'Grand Golden' papaya to partial root drying (PRD), regulated deficit irrigation (RDI) and no irrigation followed by re-hydration (NI-gh) in a greenhouse study or no irrigation (NI-field) in a field study were compared with full irrigation (Fl). In the greenhouse study, plants were grown in pot with roots split equally between two soil columns. In the field study, drip emitters on opposite sides of the plant delivered irrigation water. In Fl, the whole root system was irrigated at 100% of water use; in RDI 50% or 70% of Fl water use was supplied to the whole root system (greenhouse and field study, respectively); in PRD 100% of Fl was supplied to half the root zone while the other was allowed to dry to 50% or 70% of Fl (greenhouse, and field study, respectively). In the field study, PRD was achieved by applying different amounts of irrigation water to alternate sides of the root system within the plant row. The application of 50% water use in PRD and RDI in the greenhouse study decreased shoot and root dry weight production, with a more pronounced effect on root dry weight compared to FI. This decrease in biomass was associated with a decrease in the net photosynthetic rate in the day of most intense water stress for the plants in NI-gh. In the field study, a 30% water deficit in both PRD and RDI treatments did not significantly reduce vegetative growth or yield components (number fruit plant(-1), average weight (g) fruit(-1), kg fruit ha(-1), kg fruit plant(-1)), compared to FI. In greenhouse conditions there was evidence of a non-hydraulic signal in the PRD treatments decreasing G(s) compared to RDI at comparable soil water tension but it was insufficient to affect shoot growth or yield components in field conditions. There was no difference in the instantaneous leaf water use efficiency (WUE, A/E) of PRD or RDI treatments in the greenhouse or the agronomic water use efficiency (AVVUE) (kg fruit L-1 and number fruit L-1) in the PRD and RDI treatments in the field but both treatments improved AWUE compared to FI. It appears that papaya can tolerate some water deficits without a significant reduction in yield components indicating that <100% ET irrigation replacement can be scheduled but there is little or no benefit to PRO. (C) 2014 Elsevier B.V. All rights reserved. C1 [Nunes de Lima, Roberta Samara; Moura de Assis Figueiredo, Fabio Afonso Mazzei; Martins, Amanda Oliveira; da Silva de Deus, Bruna Correa; Ferraz, Tiago Massi; de Assis Gomes, Mara de Menezes; Campostrini, Eliemar] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, LMGV, Setor Fisiol Vegetal, BR-28013620 Campos Dos Goytacazes, RJ, Brazil. [de Sousa, Elias Fernandes] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, LEAG, Setor Irrigacao & Drenagem, BR-28013620 Campos Dos Goytacazes, RJ, Brazil. [Glenn, David Michael] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Campostrini, E (reprint author), Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, LMGV, Setor Fisiol Vegetal, Av Alberto Lamego 2000, BR-28013620 Campos Dos Goytacazes, RJ, Brazil. EM campostrini@pq.cnpq.br RI Gomes, Mara/M-7429-2016; OI Campostrini, Eliemar/0000-0002-1329-1084 FU CNPq [303633/2011-5] FX The authors wish to thank Caliman Agricola SA, Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/101534/2010), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES). The authors thank CNPq for the fellowships awarded to E. Campostrini (303633/2011-5) and E.F. de Sousa. NR 51 TC 12 Z9 12 U1 7 U2 82 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD FEB 12 PY 2015 VL 183 BP 13 EP 22 DI 10.1016/j.scienta.2014.12.005 PG 10 WC Horticulture SC Agriculture GA CA5NS UT WOS:000348955700003 ER PT J AU Xu, CP Leskovar, DI AF Xu, Chenping Leskovar, Daniel I. TI Effects of A. nodosum seaweed extracts on spinach growth, physiology and nutrition value under drought stress SO SCIENTIA HORTICULTURAE LA English DT Article DE Antioxidant capacity; Drought; Nutrition value; Photosynthesis; Seaweed; Spinach ID WATER-USE EFFICIENCY; CARBON-ISOTOPE DISCRIMINATION; ANTIOXIDANT ACTIVITY; ASCOPHYLLUM-NODOSUM; COMMERCIAL EXTRACT; LEAF-AREA; PHENOLIC CONTENT; PLANT-GROWTH; YIELD; QUALITY AB Seaweed extracts (SWE) are biodegradable and non-polluting to the environment, and have become popular as biostimulants. Their effects on spinach growth, physiology and nutrition value under drought stress were evaluated in a growth chamber study. Differential irrigations (100 and 50% evapotranspiration for full irrigation and drought stress, respectively) and Ascophyllum nodosum SWE application methods (water foliar, SWE foliar, SWE drench, SWE foliar and drench) were applied every 4 d. After 35 d, leaf growth, chlorophyll content and fluorescence, gas exchange, and nutrition value were analyzed. Under drought stress, leaf area, fresh and dry weight (FW and DW) were only 42, 42 and 60% of these under full irrigation, respectively. Drought stress also decreased leaf relative water content (RWC) and specific leaf area (SLA) from 89 to 76% and 179 to 139 cm(2) mg(-1), respectively. Gas exchange was reduced by drought stress although chlorophyll content and fluorescence were not altered. Drought stress had no direct effects on the leaf nutrition quality except for reduced ferrous ion chelating ability (FICA). Under full irrigation SINE had no effects on leaf growth, physiology and nutrition value but under drought stress SWE improved plant growth. Under drought stress A. nodosum SWE application increased leaf RWC from 76 to approximately 82% and leaf area by 16, 21 and 38% with SWE foliar spray, drench or both, respectively. Also, SWE increased leaf FW, DW and SLA by 20 to 32%, 11 to 22%, and 5 to 19%, respectively. The inhibition of gas exchange and increases of stomatal limitation induced by drought stress were reduced by SWE, irrespective of application methods. SWE application had no effects on phenolic, flavonoid, carotenoid, and antioxidant capacity, but reduced FICA. These results indicated that under mild drought stress A. nodosum SWE enhanced spinach growth by improving leaf water relations, maintaining cell turgor pressure and reducing stomatal limitation, which in turn led to large leaf area and high photosynthetic rate. A. nodosum SWE application negatively impacted nutritional quality by reduced FICA under drought stress. Published by Elsevier B.V. C1 [Xu, Chenping; Leskovar, Daniel I.] Texas A&M Univ, Texas A&M AgriLife Res, Vegetable & Fruit Improvement Ctr, Dept Hort Sci, Uvalde, TX 78801 USA. RP Xu, CP (reprint author), USDA, Agr Res Serv, 1636 E Alisal St, Salinas, CA 93905 USA. EM chenping.xu@ars.usda.gov NR 62 TC 5 Z9 5 U1 3 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD FEB 12 PY 2015 VL 183 BP 39 EP 47 DI 10.1016/j.scienta.2014.12.004 PG 9 WC Horticulture SC Agriculture GA CA5NS UT WOS:000348955700006 ER PT J AU Glenn, DM Bassett, C Dowd, SE AF Glenn, D. M. Bassett, C. Dowd, S. E. TI Effect of pest management system on 'Empire' apple leaf phyllosphere populations SO SCIENTIA HORTICULTURAE LA English DT Article DE Malus x domestica; Microbial populations; 454 technology; Kaolin ID VENTURIA-INAEQUALIS; BACTERIA; MICROORGANISMS; ADSORPTION; MINERALS; SURFACES; ECOLOGY; LEAVES; YEASTS; IMPACT AB The phyllosphere of plant tissues is varied and dynamic. Pest management, time of sampling, proximity to immigration sources, tissue and tissue status such as leaf/fruit age and location within the canopy, and other environmental and biological factors interact to influence the composition and abundance of microbial species populating the phyllosphere of apple. The purpose of this study was to examine microbial variation in the apple phyllosphere that are influenced by production management while minimizing spatial variation from widely separated sites that could introduce environmental variation. Three production systems were evaluated in adjacent 1 ha production areas: (1) conventional using synthetic fungicides and insecticides (C), (2) organic using a kaolin-based insect repellent with sulfur and lime-sulfur for disease control (ORG), and (3) the C pest management program with the addition of kaolin (CK). Apple leaves and fruit were sampled 3 times (09/07,08/08 and 09/08), washed with water to remove the microbial populations and DNA was extracted from the washed solution. DNA was probed with 16S bacterial primers and 18S fungal primers using 454 technology. Data from the top-hit BLASTn was then compiled and relative percentages of fungi at each taxonomic level were determined for each individual sample. Genus determination was based on >95% sequence identity. Simpson diversity index (D) and evenness (J) were calculated. A total of 356 bacteria and 373 fungal and yeast genera were detected in the 3 sampling studies of 3 pest management systems, however, not all genera were detected in all 3 sampling dates. Species diversity and evenness values were very small indicating very high diversity in all treatments and this is due to the detection of infrequent genera. The 3 pest management systems did not develop consistent and unique microbial populations. Nineteen bacteria and 24 fungal genera represented the core of the microbial phyllosphere diversity for the 3 sampling times. Eleven bacteria and 19 fungal genera were present in at least 50% of the plots on all sampling dates. Five bacteria (Geitlerinema, Massilia, Methylobacterium, Sphingomonas, Synechococcus) and 4 fungal (Alternaria, Aureobasidium, Catenulostroma, Phoma) genera were present in all plots on all sampling dates. In 5 samplings over 4 years, the use of kaolin in the ORG and CK treatments always increased recovery of DNA from the phyllosphere. Kaolin provided a habitat in which microbial populations were enhanced 2-5 times in both CK and ORG management systems and the recovered DNA from plant surfaces was best correlated with the growing season temperature 30-60 days before sampling. Kaolin may be a potential tool to aid in biological control efforts, by providing additional microbial habitats with reduced deleterious effects of UV on microbial populations. Published by Elsevier B.V. C1 [Glenn, D. M.; Bassett, C.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Dowd, S. E.] Res & Testing Lab, Lubbock, TX 79407 USA. RP Glenn, DM (reprint author), USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov FU USDA-ARS; NovaSource, Inc., Phoenix, AZ FX This research was supported in part by a cooperative research and development agreement between USDA-ARS and NovaSource, Inc., Phoenix, AZ. NR 23 TC 9 Z9 9 U1 4 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD FEB 12 PY 2015 VL 183 BP 58 EP 65 DI 10.1016/j.scienta.2014.12.009 PG 8 WC Horticulture SC Agriculture GA CA5NS UT WOS:000348955700008 ER PT J AU Zheng, QF Cai, ZY Ma, ZQ Gong, SQ AF Zheng, Qifeng Cai, Zhiyong Ma, Zhenqiang Gong, Shaoqin TI Cellulose Nanofibril/Reduced Graphene Oxide/Carbon Nanotube Hybrid Aerogels for Highly Flexible and All-Solid-State Supercapacitors SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE aerogels; cellulose nanofibrils; graphene; carbon nanotube; all-solid-state supercapacitor ID ELECTROCHEMICAL ENERGY-STORAGE; WALLED CARBON NANOTUBES; ORGANIC AEROGELS; PAPER; PERFORMANCE; ELECTRODES; OXIDE; CAPACITORS; BATTERIES; DEVICES AB A novel type of highly flexible and all-solid-state supercapacitor that uses cellulose nanofibril (CNF)/reduced graphene oxide (RGO)/carbon nanotube (CNT) hybrid aerogels as electrodes and H2SO4/poly(vinyl alcohol) (PVA) gel as the electrolyte was developed and is reported here. These flexible solid-state supercapacitors were fabricated without any binders, current collectors, or electroactive additives. Because of the porous structure of the CNF/RGO/CNT aerogel electrodes and the excellent electrolyte absorption properties of the CNFs present in the aerogel electrodes, the resulting flexible supercapacitors exhibited a high specific capacitance (i.e., 252 F g(-1) at a discharge current density of 0.5 A g(-1)) and a remarkable cycle stability (i.e., more than 99.5% of the capacitance was retained after 1000 charge-discharge cycles at a current density of 1 A g(-1)). Furthermore, the supercapacitors also showed extremely high areal capacitance, areal power density, and energy density (i.e., 216 mF cm(-2), 9.5 mW cm(-2), and 28.4 mu Wh cm(-2), respectively). In light of its excellent electrical performance, low cost, ease of large-scale manufacturing, and environmental friendliness, the CNF/RGO/CNT aerogel electrodes may have a promising application in the development of flexible energy-storage devices. C1 [Zheng, Qifeng; Gong, Shaoqin] Univ Wisconsin, Dept Biomed Engn, Mat Sci Program, Madison, WI 53715 USA. [Zheng, Qifeng; Gong, Shaoqin] Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI 53715 USA. [Cai, Zhiyong] United States Dept Agr, Forest Product Lab, Madison, WI 53726 USA. [Ma, Zhenqiang] Univ Wisconsin, Dept Elect Engn, Madison, WI 53706 USA. RP Gong, SQ (reprint author), Univ Wisconsin, Dept Biomed Engn, Mat Sci Program, Madison, WI 53715 USA. EM sgong@engr.wisc.edu FU University of Wisconsin-Madison; USDA Forest Products Laboratory (Madison, WI) FX The authors gratefully acknowledge the financial support of the University of Wisconsin-Madison and the USDA Forest Products Laboratory (Madison, WI). NR 63 TC 35 Z9 36 U1 57 U2 391 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD FEB 11 PY 2015 VL 7 IS 5 BP 3263 EP 3271 DI 10.1021/am507999s PG 9 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA CB4EK UT WOS:000349580800032 PM 25625769 ER PT J AU Gillman, JD Kim, WS Krishnan, HB AF Gillman, Jason D. Kim, Won-Seok Krishnan, Hari B. TI Identification of a New Soybean Kunitz Trypsin Inhibitor Mutation and Its Effect on Bowman-Birk Protease Inhibitor Content in Soybean Seed SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE soybean; Kunitz trypsin inhibitor; Bowman-Birk inhibitor; seed composition improvement ID PROTEINASE-INHIBITORS; ALPHA-CHYMOTRYPSIN; HEAT INACTIVATION; MULTIGENE FAMILY; GROWING SWINE; EXPRESSION; GLYCINE; GENE; ACCUMULATION; PREVENTS AB Soybean seed contains antinutritional compounds that inactivate digestive proteases, principally corresponding to two families: Kunitz trypsin inhibitors (KTi) and Bowman-Birk inhibitors (BBI). High levels of raw soybean/soybean meal in feed mixtures can cause poor weight gain and pancreatic abnormalities via inactivation of trypsin/chymotrypsin enzymes. Soybean protein meal is routinely heat-treated to inactivate inhibitors, a practice that is energy-intensive and costly and can degrade certain essential amino acids. In this work, we screened seed from 520 soybean accessions, using a combination of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblots with anti-Kunitz trypsin inhibitor antibodies. A soybean germplasm accession was identified with a mutation affecting an isoform annotated as nonfunctional (KTi1), which was determined to be synergistic with a previously identified mutation (KTi3-). We observed significant proteome rebalancing in all KTi mutant lines, resulting in dramatically increased BBI protein levels. C1 [Gillman, Jason D.; Kim, Won-Seok; Krishnan, Hari B.] Univ Missouri, USDA, ARS, Columbia, MO 65211 USA. RP Krishnan, HB (reprint author), Univ Missouri, USDA, ARS, 205 Curtis Hall, Columbia, MO 65211 USA. EM Hari.Krishnan@usda.ars.gov FU USDA Agricultural Research Service FX We acknowledge the superb technical assistance of Sungchan Jang, Jeremy Mullis, and Nathan Oehrle. This research was supported by the USDA Agricultural Research Service. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 43 TC 2 Z9 2 U1 3 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 11 PY 2015 VL 63 IS 5 BP 1352 EP 1359 DI 10.1021/jf505220p PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CB4CW UT WOS:000349576800005 PM 25608918 ER PT J AU Liang, JH Fu, YW Zhang, QZ Xu, DH Wang, B Lin, DJ AF Liang, Jing-Han Fu, Yao-Wu Zhang, Qi-Zhong Xu, De-Hai Wang, Bin Lin, De-Jie TI Identification and Effect of Two Flavonoids from Root Bark of Morus alba against Ichthyophthirius multifiliis in Grass Carp SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Morus alba; kuwanon G; kuwanon O; antiparasitic effect; Ichthyophthirius multifiliis ID GOLDFISH CARASSIUS-AURATUS; DIELS-ALDER ADDUCTS; MALACHITE GREEN; MEDICINAL-PLANTS; PSORALEA-CORYLIFOLIA; CHANNEL CATFISH; COPPER-SULFATE; FISH; ANTIOXIDANT; DERIVATIVES AB Ichthyophthirius multifiliis (Ich) is an important ciliate that parasitizes gills and skin of freshwater fish and causes massive fish mortality. In this study, two flavonoids (kuwanons G and O) with anti-Ich activity were isolated by bioassay-guided fractionation from the root bark of Morus alba, an important plant for sericulture. The chemical structures of kuwanons G and O were elucidated by spectroscopic analyses. Kuwanons G and O caused 100% mortality of I. multifiliis theronts at the concentration of 2 mg/L and possessed a median effective concentration (EC50) of 0.8 +/- 0.04 mg/L against the theronts. In addition, kuwanons G and O significantly reduced the infectivity of I. multifiliis theronts at concentrations of 0.125, 0.25, 0.5, and 1 mg/L. The median lethal concentrations (LC50) of kuwanons G and O to grass carp were 38.0 +/- 0.82 and 26.9 +/- 0.51 mg/L, which were approximately 50 and 35 times the EC50 for killing theronts. The results indicate that kuwanons G and O have the potential to become safe and effective drugs to control ichthyophthiriasis. C1 [Liang, Jing-Han; Fu, Yao-Wu; Zhang, Qi-Zhong; Wang, Bin; Lin, De-Jie] Jinan Univ, Key Lab Aquat Eutrophicat & Red Tide Prevent, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Guangdong Higher Educ Inst,Minist Educ,Inst Hydro, Guangzhou 510632, Guangdong, Peoples R China. [Xu, De-Hai] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Zhang, QZ (reprint author), Jinan Univ, Key Lab Aquat Eutrophicat & Red Tide Prevent, Engn Res Ctr Trop & Subtrop Aquat Ecol Engn, Guangdong Higher Educ Inst,Minist Educ,Inst Hydro, West 601 Huangpu Ave, Guangzhou 510632, Guangdong, Peoples R China. EM zhangqzdr@126.com FU National High Technology Research and Development Program of China (863 Program) [2011AA10A216]; Guangzhou Science and Technology Project [2013J4100047]; Marine and Fishery Special Project of Science and Technology in Guangdong Province [A201301B05]; Fundamental Research Funds for the Central Universities [21612111, 21613105] FX This research was supported financially by the National High Technology Research and Development Program of China (863 Program, 2011AA10A216), the Guangzhou Science and Technology Project (2013J4100047), the Marine and Fishery Special Project of Science and Technology in Guangdong Province (A201301B05), and the Fundamental Research Funds for the Central Universities (21612111 and 21613105). NR 54 TC 9 Z9 10 U1 3 U2 28 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 11 PY 2015 VL 63 IS 5 BP 1452 EP 1459 DI 10.1021/jf505544e PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CB4CW UT WOS:000349576800015 PM 25603693 ER PT J AU Kim, H Kim, DH Seo, KH Chon, JW Nah, SY Bartley, GE Arvik, T Lipson, R Yokoyama, W AF Kim, Hyunsook Kim, Dong-Hyeon Seo, Kun-ho Chon, Jung-Whan Nah, Seung-Yeol Bartley, Glenn E. Arvik, Torey Lipson, Rebecca Yokoyama, Wallace TI Modulation of the Intestinal Microbiota Is Associated with Lower Plasma Cholesterol and Weight Gain in Hamsters Fed Chardonnay Grape Seed Flour SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE microbiota; cholesterol; antibesity; FXR; grape seed flour; hamster ID REAL-TIME PCR; TOTAL PHENOLIC CONTENTS; GROUP-SPECIFIC PRIMERS; LACTIC-ACID BACTERIA; HUMAN GUT MICROBIOTA; HUMAN FECES; HYDROXYPROPYL METHYLCELLULOSE; ADIPOCYTE DIFFERENTIATION; PREDOMINANT BACTERIA; HEPATIC STEATOSIS AB The relationship between the intestinal microbiota and the hypocholesterolemic and antiobesity effects of whole grape seed flour from white and red winemaking was evaluated. Male Golden Syrian hamsters were fed a high-fat (HF) control diet or a HF diet supplemented with 10% partially defatted grape seed flours from either Chardonnay (ChrSd) or Cabernet Sauvignon (CabSd) grapes for 3 weeks. The numbers of total bacteria and relative abundances of Bifidobacterium spp., Lactobacillus spp., and Firmicutes in feces were significantly lower, while the relative abundance of Bacteroides fragilis was greater than the control from feeding the ChrSd diet. The ratio of Firmicutes/Bacteroidetes (F/B) was lower in the ChrSd diet. There were significantly positive correlations between Lactobacillus spp., ratio of F/B, and plasma total- and LDL-cholesterol and liver weight. The reduction of Lactobacillus spp. by the ChrSd diet was accompanied by inhibition of Farnesoid X receptor (FXR) signaling in the intestine as expression of intestinal fibrablast growth factor (FGF)15, positively regulated by FXR, was decreased. Expression of CYP7A1, negatively regulated by FGF15, was up-regulated in the liver, which indicates that alteration of the intestinal microbiota may regulate bile acid and lipid metabolism. These findings suggest that beneficial health effects of Chardonnay grape seed flour on HF-induced metabolic disease relate in part to modulation of intestinal microbiota and their metabolic processes. C1 [Kim, Hyunsook] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Kim, Hyunsook; Nah, Seung-Yeol] Konkuk Univ, Coll Vet Med, Dept Physiol, Seoul 143701, South Korea. [Kim, Dong-Hyeon; Seo, Kun-ho; Chon, Jung-Whan] Konkuk Univ, Coll Vet Med, KU Ctr Food Safety, Seoul 143701, South Korea. [Kim, Hyunsook; Arvik, Torey; Lipson, Rebecca] Sonomaceuticals LLC, WholeVine Prod, Santa Rosa, CA 95403 USA. [Bartley, Glenn E.; Yokoyama, Wallace] ARS, USDA, Albany, CA 94710 USA. RP Kim, H (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. EM hyunsk13@konkuk.ac.kr FU KU-Research Professor Program of Konkuk University FX Hyunsook Kim was supported by the KU-Research Professor Program of Konkuk University. NR 65 TC 6 Z9 7 U1 2 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 11 PY 2015 VL 63 IS 5 BP 1460 EP 1467 DI 10.1021/jf5026373 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CB4CW UT WOS:000349576800016 PM 25598538 ER PT J AU Veblen, KE Nehring, KC McGlone, CM Ritchie, ME AF Veblen, Kari E. Nehring, Kyle C. McGlone, Christopher M. Ritchie, Mark E. TI Contrasting Effects of Different Mammalian Herbivores on Sagebrush Plant Communities SO PLOS ONE LA English DT Article ID WESTERN NORTH-AMERICA; YELLOWSTONE-NATIONAL-PARK; NATIVE PERENNIAL GRASS; BROMUS-TECTORUM L; ELK WINTER DIETS; AFRICAN SAVANNA; GREAT-BASIN; TREE ESTABLISHMENT; STEPPE ECOSYSTEMS; POPULATION-GROWTH AB Herbivory by both grazing and browsing ungulates shapes the structure and functioning of terrestrial ecosystems worldwide, and both types of herbivory have been implicated in major ecosystem state changes. Despite the ecological consequences of differences in diets and feeding habits among herbivores, studies that experimentally distinguish effects of grazing from spatially co-occurring, but temporally segregated browsing are extremely rare. Here we use a set of long-term exclosures in northern Utah, USA, to determine how domestic grazers vs. wild ungulate herbivores (including browsers and mixed feeders) affect sagebrush-dominated plant communities that historically covered similar to 62 million ha in North America. We sampled plant community properties and found that after 22 years grazing and browsing elicited perceptible changes in overall plant community composition and distinct responses by individual plant species. In the woody layer of the plant community, release from winter and spring wild ungulate herbivory increased densities of larger Wyoming big sagebrush (Artemisia tridentata, ssp. wyomingensis) at the expense of small sagebrush, while disturbance associated with either cattle or wild ungulate activity alone was sufficient to increase bare ground and reduce cover of biological soil crusts. The perennial bunchgrass, bottlebrush squirretail (Elymus elymoides), responded positively to release from summer cattle grazing, and in turn appeared to competitively suppress another more grazing tolerant perennial grass, Sandberg's blue grass (Poa secunda). Grazing by domestic cattle also was associated with increased non-native species biomass. Together, these results illustrate that ungulate herbivory has not caused sagebrush plant communities to undergo dramatic state shifts; however clear, herbivore-driven shifts are evident. In a dry, perennial- dominated system where plant community changes can occur very slowly, our results provide insights into potential long-term trajectories of these plant communities under different large herbivore regimes. Our results can be used to guide long-term management strategies for sagebrush systems and improve habitat for endemic wildlife species such as sage-grouse (Centrocercus spp.). C1 [Veblen, Kari E.; Nehring, Kyle C.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Veblen, Kari E.; Nehring, Kyle C.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [McGlone, Christopher M.] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. [Ritchie, Mark E.] Syracuse Univ, Dept Biol, Syracuse, NY 13244 USA. RP Veblen, KE (reprint author), Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. EM kari.veblen@usu.edu RI Veblen, Kari/D-1439-2012 OI Veblen, Kari/0000-0001-7523-3368 FU Deseret Land and Livestock; Utah Agricultural Experiment Station; Utah Agricultural Experiment Station Mineral Lease Funds; USDA-ARS Pollinating Insect Research Unit; National Science Foundation DEB [0087201, 0244247] FX This work was supported by the Deseret Land and Livestock http://www.deseretlandandlivestock.com/(MER); Utah Agricultural Experiment Station http://uaes.usu.edu/(KEV); Utah Agricultural Experiment Station Mineral Lease Funds (MER); USDA-ARS Pollinating Insect Research Unit http://www.ars.usda.gov/main/site_main.htm?modecode=20-80-05-00(CMM); and the National Science Foundation DEB grants 0087201 and 0244247 (MER). The funders had no role in data collection and analysis, decision to publish, or preparation of the manuscript. With the exception of Deseret Land and Livestock, the funders had no role in study design. Deseret Land and Livestock identified general locations for the exclosures so we could focus the study on known winter ungulate range. However, the specific locations of different treatments were chosen by the authors. NR 104 TC 2 Z9 2 U1 9 U2 75 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 11 PY 2015 VL 10 IS 2 AR UNSP e0118016 DI 10.1371/journal.pone.0118016 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB3RF UT WOS:000349545300079 PM 25671428 ER PT J AU Liu, ZS Sharma, BK Erhan, SZ Biswas, A Wang, RP Schuman, TP AF Liu, Zengshe Sharma, Brajendra K. Erhan, Sevim Z. Biswas, Atanu Wang, Rongpeng Schuman, Thomas P. TI Oxidation and low temperature stability of polymerized soybean oil-based lubricants SO THERMOCHIMICA ACTA LA English DT Article DE Soybean oil; Polymer; PDSC; Oxidation stability; Low temperature stability ID KINETICS AB Oxidation and low temperature stability of polymerized soybean oil (PSO)-based lubricants have been investigated by the pressurized differential scanning calorimetry (PDSC) method. It was found that PSO samples have lower oxidative stability than their precursor, soybean oil. The main reason for the decreased stability is the generation of tertiary carbons during polymerization. By using antioxidant additives, the PSO samples responded very well and increased their onset temperature (OT) by 70-80 degrees C. Thermogravimetric analyses have been performed on the PSO samples and it was observed that they are thermally stable at temperatures up to 250 degrees C. Cold flow property data shows that the PSO samples are good for use in formulating high temperature lubricants. Two model compounds which have terminal double bonds, triacyl-10-undecenoate and allyl 10-undecenoate, have been polymerized. The oxidation stability of these two compounds was investigated by the PDSC method and compared to that of the PSO samples. This study will help us understand the relationships between oxidation stability and molecular structure of PSO-based lubricants. In addition, oxidation stability data of polyethylene (PE) and polypropylene (PP) were used in helping to explain the oxidation stability results of the PSO samples. Published by Elsevier B.V. C1 [Liu, Zengshe; Biswas, Atanu] ARS, USDA, NCAUR, Bio Oils Res, Peoria, IL 61604 USA. [Sharma, Brajendra K.] Univ Illinois, Illinois Sustainable Technol Ctr, Champaign, IL 61820 USA. [Erhan, Sevim Z.] ARS, USDA, ERRC, Wyndmoor, PA 19038 USA. [Wang, Rongpeng; Schuman, Thomas P.] Missouri Univ Sci & Technol, Dept Chem, Rolla, MO 65409 USA. RP Liu, ZS (reprint author), USDA, Peoria, IL 61604 USA. EM Kevin.liu@ars.usda.gov NR 20 TC 5 Z9 5 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0040-6031 EI 1872-762X J9 THERMOCHIM ACTA JI Thermochim. Acta PD FEB 10 PY 2015 VL 601 BP 9 EP 16 DI 10.1016/j.tca.2014.12.010 PG 8 WC Thermodynamics; Chemistry, Analytical; Chemistry, Physical SC Thermodynamics; Chemistry GA CB3DI UT WOS:000349508000002 ER PT J AU Brettin, T Davis, JJ Disz, T Edwards, RA Gerdes, S Olsen, GJ Olson, R Overbeek, R Parrello, B Pusch, GD Shukla, M Thomason, JA Stevens, R Vonstein, V Wattam, AR Xia, FF AF Brettin, Thomas Davis, James J. Disz, Terry Edwards, Robert A. Gerdes, Svetlana Olsen, Gary J. Olson, Robert Overbeek, Ross Parrello, Bruce Pusch, Gordon D. Shukla, Maulik Thomason, James A., III Stevens, Rick Vonstein, Veronika Wattam, Alice R. Xia, Fangfang TI RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes SO SCIENTIFIC REPORTS LA English DT Article ID MICROBIAL GENOMES; RNA GENES; RESISTANCE; DATABASE; SYSTEM; IDENTIFICATION; GENERATION; SEQUENCES; RESOURCE; ARCHAEA AB The RAST (Rapid Annotation using Subsystem Technology) annotation engine was built in 2008 to annotate bacterial and archaeal genomes. It works by offering a standard software pipeline for identifying genomic features (i.e., protein-encoding genes and RNA) and annotating their functions. Recently, in order to make RAST a more useful research tool and to keep pace with advancements in bioinformatics, it has become desirable to build a version of RAST that is both customizable and extensible. In this paper, we describe the RAST tool kit (RASTtk), a modular version of RAST that enables researchers to build custom annotation pipelines. RASTtk offers a choice of software for identifying and annotating genomic features as well as the ability to add custom features to an annotation job. RASTtk also accommodates the batch submission of genomes and the ability to customize annotation protocols for batch submissions. This is the first major software restructuring of RAST since its inception. C1 [Brettin, Thomas; Davis, James J.; Gerdes, Svetlana; Overbeek, Ross; Parrello, Bruce; Pusch, Gordon D.; Stevens, Rick; Vonstein, Veronika] Argonne Natl Lab, Argonne, IL 60439 USA. [Brettin, Thomas; Davis, James J.; Olson, Robert; Stevens, Rick; Xia, Fangfang] Univ Chicago, Computat Inst, Chicago, IL 60637 USA. [Disz, Terry; Gerdes, Svetlana; Overbeek, Ross; Parrello, Bruce; Pusch, Gordon D.; Vonstein, Veronika] Fellowship Interpretat Genomes, Burr Ridge, IL 60527 USA. [Edwards, Robert A.; Olson, Robert; Xia, Fangfang] Argonne Natl Lab, Div Math & Comp Sci, Argonne, IL 60439 USA. [Edwards, Robert A.] San Diego State Univ, Dept Comp Sci, San Diego, CA 92182 USA. [Olsen, Gary J.] Univ Illinois, Dept Microbiol, Urbana, IL USA. [Shukla, Maulik; Wattam, Alice R.] Virginia Tech Univ, Virginia Bioinformat Inst, Blacksburg, VA 24060 USA. [Thomason, James A., III] USDA ARS, Lab Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Stevens, Rick] Univ Chicago, Dept Comp Sci, Chicago, IL 60637 USA. RP Davis, JJ (reprint author), Argonne Natl Lab, 9700 S Cass Ave, Argonne, IL 60439 USA. EM jimdavis@uchicago.edu FU United States National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Service [HHSN272201400027C]; United States Department of Energy, DOE Systems Biology Knowledgebase [DE-AC02-06CH11357]; United States National Science Foundation [CNS-1305112]; Experimental and Computational Determination of Microbial Genotypes and Phenotypes [MCB-1330800]; National Aeronautics and Space Administration through the NASA Astrobiology Institute [NNA13AA91A] FX We thank Emily Dietrich for her helpful comments. This work was supported by the United States National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Service [Contract No. HHSN272201400027C]; the United States Department of Energy [DE-AC02-06CH11357], as part of the DOE Systems Biology Knowledgebase; R.A.E. was supported by United States National Science Foundation Grants grants II-EN: Computational Enhancement of Analytical Metagenomics Systems CNS-1305112, and Experimental and Computational Determination of Microbial Genotypes and Phenotypes MCB-1330800; and G.J.O. was supported by the National Aeronautics and Space Administration through the NASA Astrobiology Institute under Cooperative Agreement No. NNA13AA91A issued through the Science Mission Directorate. United States Department of Energy: National Institute of Allergy and Infectious Diseases. NR 40 TC 51 Z9 52 U1 4 U2 20 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 10 PY 2015 VL 5 AR 8365 DI 10.1038/srep08365 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA8LV UT WOS:000349172800009 PM 25666585 ER PT J AU Wyckoff, AC Galloway, N Meyerett-Reid, C Powers, J Spraker, T Monello, RJ Pulford, B Wild, M Antolin, M VerCauteren, K Zabel, M AF Wyckoff, A. Christy Galloway, Nathan Meyerett-Reid, Crystal Powers, Jenny Spraker, Terry Monello, Ryan J. Pulford, Bruce Wild, Margaret Antolin, Michael VerCauteren, Kurt Zabel, Mark TI Prion Amplification and Hierarchical Bayesian Modeling Refine Detection of Prion Infection SO SCIENTIFIC REPORTS LA English DT Article ID CHRONIC WASTING DISEASE; CERVUS-ELAPHUS-NELSONI; ROCKY-MOUNTAIN ELK; MISFOLDING CYCLIC AMPLIFICATION; DEER ODOCOILEUS-HEMIONUS; MULE DEER; SPONGIFORM ENCEPHALOPATHY; ANTEMORTEM DETECTION; PRP GENOTYPES; RECTAL MUCOSA AB Prions are unique infectious agents that replicate without a genome and cause neurodegenerative diseases that include chronic wasting disease (CWD) of cervids. Immunohistochemistry (IHC) is currently considered the gold standard for diagnosis of a prion infection but may be insensitive to early or sub-clinical CWD that are important to understanding CWD transmission and ecology. We assessed the potential of serial protein misfolding cyclic amplification (sPMCA) to improve detection of CWD prior to the onset of clinical signs. We analyzed tissue samples from free-ranging Rocky Mountain elk (Cervus elaphus nelsoni) and used hierarchical Bayesian analysis to estimate the specificity and sensitivity of IHC and sPMCA conditional on simultaneously estimated disease states. Sensitivity estimates were higher for sPMCA (99.51%, credible interval (CI) 97.15-100%) than IHC of obex (brain stem, 76.56%, CI 57.00-91.46%) or retropharyngeal lymph node (90.06%, CI 74.13-98.70%) tissues, or both (98.99%, CI 90.01-100%). Our hierarchical Bayesian model predicts the prevalence of prion infection in this elk population to be 18.90% (CI 15.50-32.72%), compared to previous estimates of 12.90%. Our data reveal a previously unidentified sub-clinical prion-positive portion of the elk population that could represent silent carriers capable of significantly impacting CWD ecology. C1 [Wyckoff, A. Christy; Meyerett-Reid, Crystal; Spraker, Terry; Pulford, Bruce; Zabel, Mark] Colorado State Univ, Coll Vet Med & Biomed Sci, Prion Res Ctr, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Wyckoff, A. Christy; VerCauteren, Kurt] USDA, Natl Wildlife Res Ctr, Wildlife Serv, Ft Collins, CO 80521 USA. [Galloway, Nathan; Antolin, Michael] Colorado State Univ, Coll Nat Sci, Dept Biol, Ft Collins, CO 80523 USA. [Powers, Jenny; Monello, Ryan J.; Wild, Margaret] Biol Resource Management Div, Natl Pk Serv, Ft Collins, CO 80525 USA. RP Zabel, M (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Prion Res Ctr, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. EM mark.zabel@colostate.edu FU National Wildlife Research Center-United States Department of Agriculture; National Science Foundation [EF-0914489] FX We thank the National Park Service for access to samples and IHC result, Dr. M. Hooten for his assistance with model design, the National Wildlife Research Center-United States Department of Agriculture and the National Science Foundation (grant EF-0914489) for funding and support of this research. NR 58 TC 2 Z9 2 U1 8 U2 31 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 10 PY 2015 VL 5 AR 8358 DI 10.1038/srep08358 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA8LV UT WOS:000349172800002 PM 25665713 ER PT J AU Kistler, HC Broz, K AF Kistler, H. Corby Broz, Karen TI Cellular compartmentalization of secondary metabolism SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE aflatoxin; deoxynivalenol; mycotoxin; non-ribosomal peptide; polyketide; penicillin; terpene ID HMG-COA REDUCTASE; SUBCELLULAR-LOCALIZATION; ASPERGILLUS-PARASITICUS; PENICILLIUM-CHRYSOGENUM; ENDOPLASMIC-RETICULUM; FILAMENTOUS FUNGUS; FUSARIUM-SPOROTRICHIOIDES; FRACTIONATED COLONIES; FUNCTIONAL EXPRESSION; BETA-OXIDATION AB Fungal secondary metabolism is often considered apart from the essential housekeeping functions of the cell. However, there are clear links between fundamental cellular metabolism and the biochemical pathways leading to secondary metabolite synthesis. Besides utilizing key biochemical precursors shared with the most essential processes of the cell (e.g., amino acids, acetyl CoA, NADPH), enzymes for secondary metabolite synthesis are compartmentalized at conserved subcellular sites that position pathway enzymes to use these common biochemical precursors. Co-compartmentalization of secondary metabolism pathway enzymes also may function to channel precursors, promote pathway efficiency and sequester pathway intermediates and products from the rest of the cell. In this review we discuss the compartmentalization of three well-studied fungal secondary metabolite biosynthetic pathways for penicillin G, aflatoxin and deoxynivalenol, and summarize evidence used to infersubcellular localization. We also discuss how these metabolites potentially are trafficked within the cell and may be exported. C1 [Kistler, H. Corby; Broz, Karen] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kistler, HC (reprint author), Univ Minnesota, USDA ARS, Cereal Dis Lab, 1551 Lindig St, St Paul, MN 55108 USA. EM hckist@umn.edu OI Broz, Karen/0000-0001-7928-7216; Kistler, Harold/0000-0001-5312-6297 FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2014-67013-21561]; Guillermo Marques at the University of Minnesota-University Imaging Centers FX We are grateful to Dr. Marike Boenisch for her helpful suggestions to the manuscript. This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2014-67013-21561 from the USDA National Institute of Food and Agriculture. Microscopy was conducted using a Zeiss Cell Observer SD spinning disk confocal microscope and support by Guillermo Marques at the University of Minnesota-University Imaging Centers, http://uic.umn.edu. NR 60 TC 14 Z9 14 U1 5 U2 57 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD FEB 9 PY 2015 VL 6 AR 68 DI 10.3389/fmicb.2015.00068 PG 11 WC Microbiology SC Microbiology GA CC0KD UT WOS:000350023300001 PM 25709603 ER PT J AU Cote, R Katani, R Moreau, MR Kudva, IT Arthur, TM DebRoy, C Mwangi, MM Albert, I Garay, JAR Li, LL Brandl, MT Carter, MQ Kapur, V AF Cote, Rebecca Katani, Robab Moreau, Matthew R. Kudva, Indira T. Arthur, Terrance M. DebRoy, Chitrita Mwangi, Michael M. Albert, Istvan Garay, Juan Antonio Raygoza Li, Lingling Brandl, Maria T. Carter, Michelle Q. Kapur, Vivek TI Comparative Analysis of Super-Shedder Strains of Escherichia coli O157:H7 Reveals Distinctive Genomic Features and a Strongly Aggregative Adherent Phenotype on Bovine Rectoanal Junction Squamous Epithelial Cells SO PLOS ONE LA English DT Article ID EFFACEMENT-ENCODED PROTEINS; TERMINAL RECTAL MUCOSA; PHYLOGENETIC GROUP; HUMAN INFECTION; FEEDLOT CATTLE; UNITED-STATES; O157-H7; COLONIZATION; VIRULENCE; GENE AB Shiga toxin-producing Escherichia coli O157:H7 (O157) are significant foodborne pathogens and pose a serious threat to public health worldwide. The major reservoirs of O157 are asymptomatic cattle which harbor the organism in the terminal recto-anal junction (RAJ). Some colonized animals, referred to as "super-shedders" (SS), are known to shed O157 in exceptionally large numbers (> 104 CFU/g of feces). Recent studies suggest that SS cattle play a major role in the prevalence and transmission of O157, but little is known about the molecular mechanisms associated with super-shedding. Whole genome sequence analysis of an SS O157 strain (SS17) revealed a genome of 5,523,849 bp chromosome with 5,430 open reading frames and two plasmids, pO157 and pSS17, of 94,645 bp and 37,446 bp, respectively. Comparative analyses showed that SS17 is clustered with spinach-associated O157 outbreak strains, and belongs to the lineage I/II, clade 8, D group, and genotype 1, a subgroup of O157 with predicted hyper-virulence. A large number of non-synonymous SNPs and other polymorphisms were identified in SS17 as compared with other O157 strains (EC4115, EDL933, Sakai, TW14359), including in key adherence-and virulence-related loci. Phenotypic analyses revealed a distinctive and strongly adherent aggregative phenotype of SS17 on bovine RAJ stratified squamous epithelial (RSE) cells that was conserved amongst other SS isolates. Molecular genetic and functional analyses of defined mutants of SS17 suggested that the strongly adherent aggregative phenotype amongst SS isolates is LEE-independent, and likely results from a novel mechanism. Taken together, our study provides a rational framework for investigating the molecular mechanisms associated with SS, and strong evidence that SS O157 isolates have distinctive features and use a LEE-independent mechanism for hyper-adherence to bovine rectal epithelial cells. C1 [Cote, Rebecca; Katani, Robab; Moreau, Matthew R.; DebRoy, Chitrita; Garay, Juan Antonio Raygoza; Li, Lingling; Kapur, Vivek] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Cote, Rebecca; Albert, Istvan; Garay, Juan Antonio Raygoza; Kapur, Vivek] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA. [Kudva, Indira T.] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Arthur, Terrance M.] ARS, Roman L Hruska US Meat Anim Res Ctr, USDA, Clay Ctr, NE USA. [DebRoy, Chitrita] Penn State Univ, E Coli Reference Ctr, University Pk, PA 16802 USA. [Mwangi, Michael M.; Albert, Istvan] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [Brandl, Maria T.; Carter, Michelle Q.] ARS, Res Unit, USDA, Albany, CA USA. RP Kapur, V (reprint author), Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. EM vkapur@psu.edu NR 85 TC 4 Z9 4 U1 2 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 9 PY 2015 VL 10 IS 2 AR e0116743 DI 10.1371/journal.pone.0116743 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA7UO UT WOS:000349123100013 PM 25664460 ER PT J AU Qualls, WA Muller, GC Khallaayoune, K Revay, EE Zhioua, E Kravchenko, VD Arheart, KL Xue, RD Schlein, Y Hausmann, A Kline, DL Beier, JC AF Qualls, Whitney A. Mueller, Gunter C. Khallaayoune, Khalid Revay, Edita E. Zhioua, Elyes Kravchenko, Vasiliy D. Arheart, Kristopher L. Xue, Rui-De Schlein, Yosef Hausmann, Axel Kline, Daniel L. Beier, John C. TI Control of sand flies with attractive toxic sugar baits (ATSB) and potential impact on non-target organisms in Morocco SO PARASITES & VECTORS LA English DT Article DE Integrated vector management; Leishmaniasis; Vector control; Vector-borne diseases; Environmental impact ID PHLEBOTOMUS-PAPATASI; MALARIA VECTORS; LEISHMANIASIS; DIPTERA; PSYCHODIDAE; POPULATIONS; MOSQUITOS; ENVIRONMENTS; INSECTICIDE; DINOTEFURAN AB Background: The persistence and geographical expansion of leishmaniasis is a major public health problem that requires the development of effective integrated vector management strategies for sand fly control. Moreover, these strategies must be economically and environmentally sustainable approaches that can be modified based on the current knowledge of sand fly vector behavior. The efficacy of using attractive toxic sugar baits (ATSB) for sand fly control and the potential impacts of ATSB on non-target organisms in Morocco was investigated. Methods: Sand fly field experiments were conducted in an agricultural area along the flood plain of the Ourika River. Six study sites (600 m x 600 m); three with "sugar rich" (with cactus hedges bearing countless ripe fruits) environments and three with "sugar poor" (green vegetation only suitable for plant tissue feeding) environments were selected to evaluate ATSB, containing the toxin, dinotefuran. ATSB applications were made either with bait stations or sprayed on non-flowering vegetation. Control sites were established in both sugar rich and sugar poor environments. Field studies evaluating feeding on vegetation treated with attractive (non-toxic) sugar baits (ASB) by non-target arthropods were conducted at both sites with red stained ASB applied to non-flowering vegetation, flowering vegetation, or on bait stations. Results: At both the sites, a single application of ATSB either applied to vegetation or bait stations significantly reduced densities of both female and male sand flies (Phlebotomus papatasi and P. sergenti) for the five-week trial period. Sand fly populations were reduced by 82.8% and 76.9% at sugar poor sites having ATSB applied to vegetation or presented as a bait station, respectively and by 78.7% and 83.2%, respectively at sugar rich sites. The potential impact of ATSB on non-targets, if applied on green non-flowering vegetation and bait stations, was low for all non-target groups as only 1% and 0.7% were stained with non-toxic bait respectively when monitored after 24 hours. Conclusions: The results of this field study demonstrate ATSB effectively controls both female and male sand flies regardless of competing sugar sources. Furthermore, ATSB applied to foliar vegetation and on bait stations has low non-target impact. C1 [Qualls, Whitney A.; Arheart, Kristopher L.; Beier, John C.] Univ Miami, Miller Sch Med, Dept Publ Hlth Sci, Miami, FL 33136 USA. [Mueller, Gunter C.; Schlein, Yosef] Hebrew Univ Jerusalem, Fac Med, Kuvin Ctr Study Infect & Trop Dis, Dept Microbiol & Mol Genet, IL-91120 Jerusalem, Israel. [Khallaayoune, Khalid] Rabat Inst, Inst Agron & Vet Hassan II, Dept Parasitol, Rabat, Morocco. [Revay, Edita E.] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Anat & Cell Biol, IL-34995 Haifa, Israel. [Zhioua, Elyes] Pasteur Inst Tunis, Lab Vector Ecol, Tunis 1002, Tunisia. [Kravchenko, Vasiliy D.] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel. [Xue, Rui-De] Anastasia Mosquito Control Dist, St Augustine, FL 32080 USA. [Hausmann, Axel] SNSB Zool Staatssammlung Munchen, Munich, Germany. [Kline, Daniel L.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Qualls, WA (reprint author), Univ Miami, Miller Sch Med, Dept Publ Hlth Sci, Miami, FL 33136 USA. EM w.qualls@med.miami.edu FU National Institute of Allergy And Infectious Diseases of the National Institutes of Health [R01AI100968]; United States Department of the Army, Space and Missile Defense Command, Deployed War Fighters Protection Project (DWFP) grant [W81R6091285002] FX Special thanks are due to the personnel of the Department of Agriculture in Marrakech, Morocco and the local authorities for the valuable assistance. The research reported in this publication was supported by the National Institute of Allergy And Infectious Diseases of the National Institutes of Health under Award Number R01AI100968 and the United States Department of the Army, Space and Missile Defense Command, Deployed War Fighters Protection Project (DWFP) grant# W81R6091285002. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or the Deployed War Fighter program. NR 43 TC 2 Z9 2 U1 2 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD FEB 8 PY 2015 VL 8 AR 87 DI 10.1186/s13071-015-0671-2 PG 9 WC Parasitology SC Parasitology GA CC2LF UT WOS:000350175800001 PM 25890039 ER PT J AU LeCain, D Smith, D Morgan, J Kimball, BA Pendall, E Miglietta, F AF LeCain, Daniel Smith, David Morgan, Jack Kimball, Bruce A. Pendall, Elise Miglietta, Franco TI Microclimatic Performance of a Free-Air Warming and CO2 Enrichment Experiment in Windy Wyoming, USA SO PLOS ONE LA English DT Article ID ELEVATED ATMOSPHERIC CO2; INFRARED HEATER ARRAYS; WATER RELATIONS; PHOTOSYNTHETIC RESPONSES; NORTH-AMERICA; C-4 GRASSES; FIELD PLOTS; ECOSYSTEM; GRASSLAND; SOIL AB In order to plan for global changing climate experiments are being conducted in many countries, but few have monitored the effects of the climate change treatments (warming, elevated CO2) on the experimental plot microclimate. During three years of an eight year study with year-round feedback-controlled infra-red heater warming (1.5/3.0 degrees C day/night) and growing season free-air CO2 enrichment (600 ppm) in the mixed-grass prairie of Wyoming, USA, we monitored soil, leaf, canopy-air, above-canopy-air temperatures and relative humidity of control and treated experimental plots and evaluated ecologically important temperature differentials. Leaves were warmed somewhat less than the target settings (1.1 & 1.5 degrees C day/night) but soil was warmed more creating an average that matched the target settings extremely well both during the day and night plus the summer and winter. The site typically has about 50% bare or litter covered soil, therefore soil heat transfer is more critical than in dense canopy ecosystems. The Wyoming site commonly has strong winds (5 ms(-1) average) and significant daily and seasonal temperature fluctuations (as much as 30 degrees C daily) but the warming system was nearly always able to maintain the set temperatures regardless of abiotic variation. The within canopy-air was only slightly warmed and above canopy-air was not warmed by the system, therefore convective warming was minor. Elevated CO2 had no direct effect nor interaction with the warming treatment on microclimate. Relative humidity within the plant canopy was only slightly reduced by warming. Soil water content was reduced by warming but increased by elevated CO2. This study demonstrates the importance of monitoring the microclimate in manipulative field global change experiments so that critical physiological and ecological conclusions can be determined. Highly variable energy demand fluctuations showed that passive IR heater warming systems will not maintain desired warming for much of the time. C1 [LeCain, Daniel; Smith, David; Morgan, Jack] ARS, USDA, Rangelands Resources Res Unit, Northern Plains Area, Ft Collins, CO 80521 USA. [Kimball, Bruce A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Pendall, Elise] Univ Western Sydney, Hawkesbury Inst Environm, Sydney, NSW, Australia. [Miglietta, Franco] Ist Biometeorol, Florence, Italy. RP LeCain, D (reprint author), ARS, USDA, Rangelands Resources Res Unit, Northern Plains Area, Ft Collins, CO 80521 USA. EM dan.lecain@ars.usda.gov RI Miglietta, Franco/A-1257-2009; OI Miglietta, Franco/0000-0003-1474-8143; Pendall, Elise/0000-0002-1651-8969 FU US Department of Agriculture Agricultural Research Service Climate Change, Soils & Emissions Program; USDA-CSREES Soil Processes Program [2008-35107-18655]; US Department of Energy Office of Science (BER), through Terrestrial Ecosystem Science program [DE-SC0006973]; Western Regional Center of the National Institute for Climatic Change Research; National Science Foundation (DEB) [1021559] FX This material is based upon work supported by the US Department of Agriculture Agricultural Research Service Climate Change, Soils & Emissions Program, USDA-CSREES Soil Processes Program (#2008-35107-18655), US Department of Energy Office of Science (BER), through the Terrestrial Ecosystem Science program (#DE-SC0006973) and the Western Regional Center of the National Institute for Climatic Change Research, and by the National Science Foundation (DEB#1021559). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 34 TC 7 Z9 7 U1 7 U2 35 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 6 PY 2015 VL 10 IS 2 AR e0116834 DI 10.1371/journal.pone.0116834 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB2GK UT WOS:000349444900071 PM 25658313 ER PT J AU Tiezzi, F Parker-Gaddis, KL Cole, JB Clay, JS Maltecca, C AF Tiezzi, Francesco Parker-Gaddis, Kristen L. Cole, John B. Clay, John S. Maltecca, Christian TI A Genome-Wide Association Study for Clinical Mastitis in First Parity US Holstein Cows Using Single-Step Approach and Genomic Matrix Re-Weighting Procedure SO PLOS ONE LA English DT Article ID MAMMARY EPITHELIAL-CELLS; QUANTITATIVE TRAIT LOCI; MAPPING INCLUDING PHENOTYPES; LARGE GENE LISTS; FACTOR-KAPPA-B; DAIRY-CATTLE; STAPHYLOCOCCUS-AUREUS; STREPTOCOCCUS-UBERIS; CANDIDATE GENES; ESCHERICHIA-COLI AB Clinical mastitis (CM) is one of the health disorders with large impacts on dairy farming profitability and animal welfare. The objective of this study was to perform a genome-wide association study (GWAS) for CM in first-lactation Holstein. Producer-recorded mastitis event information for 103,585 first-lactation cows were used, together with genotype information on 1,361 bulls from the Illumina BovineSNP50 BeadChip. Single-step genomic-BLUP methodology was used to incorporate genomic data into a threshold-liability model. Association analysis confirmed that CM follows a highly polygenic mode of inheritance. However, 10-adjacent-SNP windows showed that regions on chromosomes 2, 14 and 20 have impacts on genetic variation for CM. Some of the genes located on chromosome 14 (LY6K, LY6D, LYNX1, LYPD2, SLURP1, PSCA) are part of the lymphocyte-antigen-6 complex (LY6) known for its neutrophil regulation function linked to the major histocompatibility complex. Other genes on chromosome 2 were also involved in regulating immune response (IFIH1, LY75, and DPP4), or are themselves regulated in the presence of specific pathogens (ITGB6, NR4A2). Other genes annotated on chromosome 20 are involved in mammary gland metabolism (GHR, OXCT1), antibody production and phagocytosis of bacterial cells (C6, C7, C9, C1QTNF3), tumor suppression (DAB2), involution of mammary epithelium (OSMR) and cytokine regulation (PRLR). DAVID enrichment analysis revealed 5 KEGG pathways. The JAK-STAT signaling pathway (cell proliferation and apoptosis) and the 'Cytokine-cytokine receptor interaction' (cytokine and interleukines response to infectious agents) are co-regulated and linked to the 'ABC transporters' pathway also found here. Gene network analysis performed using GeneMania revealed a co-expression network where 665 interactions existed among 145 of the genes reported above. Clinical mastitis is a complex trait and the different genes regulating immune response are known to be pathogen-specific. Despite the lack of information in this study, candidate QTL for CM were identified in the US Holstein population. C1 [Tiezzi, Francesco; Parker-Gaddis, Kristen L.; Maltecca, Christian] N Carolina State Univ, Dept Anim Sci, Raleigh, NC 27695 USA. [Parker-Gaddis, Kristen L.; Cole, John B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Clay, John S.] Dairy Records Management Syst, Raleigh, NC 27603 USA. RP Tiezzi, F (reprint author), N Carolina State Univ, Dept Anim Sci, Box 7621, Raleigh, NC 27695 USA. EM ftmaestr@ncsu.edu RI Tiezzi, Francesco/L-6411-2016; Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Select Sires [2010-1714] FX This project was supported by project 2010-1714 from Select Sires - The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials. NR 87 TC 6 Z9 6 U1 3 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 6 PY 2015 VL 10 IS 2 AR e0114919 DI 10.1371/journal.pone.0114919 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB2GK UT WOS:000349444900011 PM 25658712 ER PT J AU Jhung, MA Nelson, DI AF Jhung, Michael A. Nelson, Deborah I. TI Outbreaks of Avian Influenza A (H5N2), (H5N8), and (H5N1) Among Birds - United States, December 2014-January 2015 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Editorial Material ID INFECTION C1 [Jhung, Michael A.] CDC, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. [Nelson, Deborah I.] US Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO USA. RP Jhung, MA (reprint author), CDC, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. EM mjhung@cdc.gov NR 3 TC 40 Z9 40 U1 0 U2 19 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD FEB 6 PY 2015 VL 64 IS 4 BP 111 EP 111 PG 1 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CA6JP UT WOS:000349019200008 PM 25654614 ER PT J AU Samayoa, LF Malvar, RA Olukolu, BA Holland, JB Butron, A AF Samayoa, Luis Fernando Malvar, Rosa Ana Olukolu, Bode A. Holland, James B. Butron, Ana TI Genome-wide association study reveals a set of genes associated with resistance to the Mediterranean corn borer (Sesamia nonagrioides L.) in a maize diversity panel SO BMC PLANT BIOLOGY LA English DT Article DE Candidate genes; Corn borer; Genome-wide association study; Insect resistance; Maize; Mixed linear models; Sesamia nonagrioides ID PINK STEM BORER; DEPENDENT PROTEIN-KINASE; QUANTITATIVE TRAIT LOCI; GLYCINE-RICH PROTEINS; GERMIN-LIKE PROTEINS; PLANT-CELL WALLS; TRANSCRIPTION FACTORS; TROPICAL MAIZE; LEPIDOPTERA-NOCTUIDAE; PSEUDOMONAS-SYRINGAE AB Background: Corn borers are the primary maize pest; their feeding on the pith results in stem damage and yield losses. In this study, we performed a genome-wide association study (GWAS) to identify SNPs associated with resistance to Mediterranean corn borer in a maize diversity panel using a set of more than 240,000 SNPs. Results: Twenty five SNPs were significantly associated with three resistance traits: 10 were significantly associated with tunnel length, 4 with stem damage, and 11 with kernel resistance. Allelic variation at each significant SNP was associated with from 6 to 9% of the phenotypic variance. A set of genes containing or physically close to these SNPs are proposed as candidate genes for borer resistance, supported by their involvement in plant defense-related mechanisms in previously published evidence. The linkage disequilibrium decayed (r(2) < 0.10) rapidly within short distance, suggesting high resolution of GWAS associations. Conclusions: Most of the candidate genes found in this study are part of signaling pathways, others act as regulator of expression under biotic stress condition, and a few genes are encoding enzymes with antibiotic effect against insects such as the cystatin1 gene and the defensin proteins. These findings contribute to the understanding the complex relationship between plant-insect interactions. C1 [Samayoa, Luis Fernando; Malvar, Rosa Ana; Butron, Ana] CSIC, Spanish Natl Res Council, Mis Biol Galicia, Pontevedra, Spain. [Olukolu, Bode A.; Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Holland, James B.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Samayoa, LF (reprint author), CSIC, Spanish Natl Res Council, Mis Biol Galicia, POB 2836080, Pontevedra, Spain. EM fsamayoa@mbg.csic.es OI Holland, James/0000-0002-4341-9675 FU National Plan for Research and Development of Spain [AGL2012-33415]; Spanish Council for Scientific Research (CSIC) FX This work was supported by the National Plan for Research and Development of Spain (projects AGL2012-33415). L.F. Samayoa acknowledges a contract JAE-Predoc from the Spanish Council for Scientific Research (CSIC). We also acknowledges to the Super Computation Center of Galicia (CESGA) for allowed to use their serves to do the preliminary analysis of this study. We thank Mike McMullen for inspiring this study and reviewing the manuscript. NR 150 TC 11 Z9 12 U1 22 U2 70 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD FEB 5 PY 2015 VL 15 AR 35 DI 10.1186/s12870-014-0403-3 PG 15 WC Plant Sciences SC Plant Sciences GA CC0YZ UT WOS:000350067300001 PM 25652257 ER PT J AU Simko, I Zhou, YG Brandl, MT AF Simko, Ivan Zhou, Yaguang Brandl, Maria T. TI Downy mildew disease promotes the colonization of romaine lettuce by Escherichia coli O157:H7 and Salmonella enterica SO BMC MICROBIOLOGY LA English DT Article DE Produce contamination; Bremia lactucae; Plant pathogen; Oomycete; Human pathogen; Foodborne pathogen; Enteric pathogen; Basal plant immunity ID CONTAMINATED MANURE COMPOSTS; LISTERIA-MONOCYTOGENES; PENICILLIUM-EXPANSUM; GLOMERELLA-CINGULATA; IRRIGATION WATER; O157-H7; SURVIVAL; INTERNALIZATION; RESISTANCE; PATHOGENS AB Background: Downy mildew, a plant disease caused by the oomycete Bremia lactucae, is endemic in many lettuce-growing regions of the world. Invasion by plant pathogens may create new portals and opportunities for microbial colonization of plants. The occurrence of outbreaks of Escherichia coli O157: H7 (EcO157) and Salmonella enterica Typhimurium (S. Typhimurium) infections linked to lettuce prompted us to investigate the role of downy mildew in the colonization of romaine lettuce by these human pathogens under controlled laboratory conditions. Results: Whereas both EcO157 and S. Typhimurium population sizes increased 102-fold on healthy leaf tissue under conditions of warm temperature and free water on the leaves, they increased by 105-fold in necrotic lesions caused by B. lactucae. Confocal microscopy of GFP-EcO157 in the necrotic tissue confirmed its massive population density and association with the oomycete hyphae. Multiplication of EcO157 in the diseased tissue was significantly lower in the RH08-0464 lettuce line, which has a high level of resistance to downy mildew than in the more susceptible cultivar Triple Threat. qRT-PCR quantification of expression of the plant basal immunity gene PR-1, revealed that this gene had greater transcriptional activity in line RH08-0464 than in cultivar Triple Threat, indicating that it may be one of the factors involved in the differential growth of the human pathogen in B. lactucae lesions between the two lettuce accessions. Additionally, downy mildew disease had a significant effect on the colonization of EcO157 at high relative humidity (RH 90-100%) and on its persistence at lower RH (65-75%). The latter conditions, which promoted overall dryness of the lettuce leaf surface, allowed for only 0.0011% and 0.0028% EcO157 cell survival in healthy and chlorotic tissue, respectively, whereas 1.58% of the cells survived in necrotic tissue. Conclusions: Our results indicate that downy mildew significantly alters the behavior of enteric pathogens in the lettuce phyllosphere and that breeding for resistance to B. lactucae may lower the increased risk of microbial contamination caused by this plant pathogen. C1 [Simko, Ivan] ARS, Crop Improvement & Protect Res Unit, USDA, Salinas, CA 93905 USA. [Zhou, Yaguang; Brandl, Maria T.] ARS, Prod Safety & Microbiol Res Unit, USDA, Albany, CA 94563 USA. RP Brandl, MT (reprint author), ARS, Prod Safety & Microbiol Res Unit, USDA, Albany, CA 94563 USA. EM maria.brandl@ars.usda.gov RI Simko, Ivan/J-6048-2012 OI Simko, Ivan/0000-0002-8769-8477 FU United States Department of Agriculture; Agricultural Research Service CRIS projects [5325-42000-046, 5305-21220-005] FX We thank A. Atallah for technical assistance with field experiments and R. Hayes for providing plants used in preliminary experiments. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This research was supported by the United States Department of Agriculture, Agricultural Research Service CRIS projects 5325-42000-046 and 5305-21220-005. NR 42 TC 8 Z9 8 U1 6 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD FEB 4 PY 2015 VL 15 AR 19 DI 10.1186/s12866-015-0360-5 PG 9 WC Microbiology SC Microbiology GA CC3NJ UT WOS:000350255900001 PM 25648408 ER PT J AU Di, LP Yang, ZW AF Di, Liping Yang, Zhengwei TI Special Section Guest Editorial: Remote Sensing and Sensor Networks for Promoting Agro-Geoinformatics SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Editorial Material C1 [Di, Liping] George Mason Univ, Ctr Spatial Informat Sci & Syst, Fairfax, VA 22030 USA. [Yang, Zhengwei] Natl Agr Stat Serv, USDA, Div Res & Dev, Fairfax, VA 22030 USA. RP Di, LP (reprint author), George Mason Univ, Ctr Spatial Informat Sci & Syst, 4400 Univ Dr,MS 6E1, Fairfax, VA 22030 USA. EM ldi@gmu.edu; zhengwei.yang@nass.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 5 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD FEB 4 PY 2015 VL 8 AR 085101 PG 2 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CC8QL UT WOS:000350633100001 ER PT J AU Kim, SP Nam, SH Friedman, M AF Kim, Sung Phil Nam, Seok Hyun Friedman, Mendel TI The Tomato Glycoalkaloid alpha-Tomatine Induces Caspase-Independent Cell Death in Mouse Colon Cancer CT-26 Cells and Transplanted Tumors in Mice SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE alpha-tomatine; tomatoes; CT-26 colon cancer cells; mouse tumor inhibition; body and organ weights; mechanism; research needs ID PULSED AMPEROMETRIC DETECTION; NF-KAPPA-B; LYCOPERSICON-ESCULENTUM; STEROIDAL ALKALOIDS; LYCOPENE CONTENT; BETA-CAROTENE; BEARING MICE; HUMAN BREAST; IN-VITRO; APOPTOSIS AB Tomatoes (Solanum lycopersicum) produce the bioactive glycoalkaloid alpha-tomatine. This study determined the effect of commercial alpha-tomatine on CT-26 colon cancer cells in vitro and in vivo in an intracutaneously transplanted mouse tumor. Cytotoxicity experiments showed that alpha-tomatine induces about 50% lysis of the colon cancer cells at 3.5 mu M after 24 h of treatment. Large proportions of cells were found to be in the annexin V (+)/propidium iodide (+) phase of cell death, implying late phase apoptotic/necrotic status. However, alpha-tomatine induced cell death in CT-26 cancer cells through caspase-independent signaling pathways. This conclusion was supported by Western blot analysis showing a localization of apoptosis-inducing mitochondrial protein (AIF) to the nucleus and down-regulation of survivin (an inhibitor of apoptosis) expression as well as failure to detect the active form of caspase-3, -8, and -9 produced by proteolytic cleavage in CT-26 cancer cells. Intraperitoneally administered alpha-tomatine (5 mg/kg body weight) also markedly inhibited growth of the tumor using CT-26 cancer cells without causing body and organ weight changes. The reduced tumor growth in the mice by 38% after 2 weeks was the result of increased caspase-independent apoptosis associated with increased nuclear translocation of AIF and decreased survivin expression in tumor tissues. alpha-Tomatine in pure form and in tomatine-rich green tomatoes might prevent colon cancer. C1 [Kim, Sung Phil; Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Nam, SH (reprint author), Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. EM shnam@ajou.ac.kr; mendel.friedman@ars.usda.gov NR 48 TC 6 Z9 6 U1 1 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 4 PY 2015 VL 63 IS 4 BP 1142 EP 1150 DI 10.1021/jf5040288 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CA8AG UT WOS:000349138300012 ER PT J AU Zeller, WE Sullivan, ML Mueller-Harvey, I Grabber, JH Ramsay, A Drake, C Brown, RH AF Zeller, Wayne E. Sullivan, Michael L. Mueller-Harvey, Irene Grabber, John H. Ramsay, Aina Drake, Chris Brown, Ronald H. TI Protein Precipitation Behavior of Condensed Tannins from Lotus pedunculatus and Trifolium repens with Different Mean Degrees of Polymerization SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE condensed tannin; proanthocyanidin; protein precipitation; tannin-protein complexes; nuclear magnetic resonance spectroscopy; NMR; thiolysis ID IN-VITRO; MOLECULAR-WEIGHTS; TEMPERATE FORAGES; CH4 PRODUCTION; BINDING; RUMINANTS; PHASE; QUANTIFICATION; PROCYANIDINS; AGGREGATION AB The precipitation of bovine serum albumin (BSA), lysozyme (LYS), and alfalfa leaf protein (ALF) by two large- and two medium-sized condensed tannin (CT) fractions of similar flavan-3-ol subunit composition is described. CT fractions isolated from white clover flowers and big trefoil leaves exhibited high-purity profiles by 1D/2D NMR and purities >90% (determined by thiolysis). At pH 6.5, large CTs with a mean degree of polymerization (mDP) of similar to 18 exhibited similar protein precipitation behaviors and were significantly more effective than medium CTs (mDP similar to 9). Medium CTs exhibited similar capacities to precipitate ALF or BSA, but showed small but significant differences in their capacity to precipitate LYS. All CTs precipitated ALF more effectively than BSA or LYS. Aggregation of CT-protein complexes likely aided precipitation of ALF and BSA, but not LYS. This study, one of the first to use CTs of confirmed high purity, demonstrates that the mDP of CTs influences protein precipitation efficacy. C1 [Zeller, Wayne E.; Sullivan, Michael L.; Grabber, John H.] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. [Mueller-Harvey, Irene; Ramsay, Aina; Drake, Chris; Brown, Ronald H.] Univ Reading, Sch Agr Policy & Dev, Food Prod & Qual Div, Chem & Biochem Lab, Reading RG6 6AT, Berks, England. RP Zeller, WE (reprint author), ARS, US Dairy Forage Res Ctr, USDA, 1925 Linden Dr, Madison, WI 53706 USA. EM Wayne.Zeller@ars.usda.gov FU USDA-ARS [58-3655-0-155F]; University of Reading, UK; European Union Marie Curie Initial Training Network [PITN-GA-2011-289377] FX This work was funded in part by USDA-ARS Specific Cooperative Agreement 58-3655-0-155F with the University of Reading, UK, and was supported by a European Union Marie Curie Initial Training Network (PITN-GA-2011-289377 ('LegumePlus'). Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 57 TC 6 Z9 6 U1 5 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 4 PY 2015 VL 63 IS 4 BP 1160 EP 1168 DI 10.1021/jf504715p PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CA8AG UT WOS:000349138300014 ER PT J AU Cook, D Pfister, JA Constantino, JR Roper, JM Gardner, DR Welch, KD Hammond, ZJ Green, BT AF Cook, Daniel Pfister, James A. Constantino, John R. Roper, Jessie M. Gardner, Dale R. Welch, Kevin D. Hammond, Zachary J. Green, Benedict T. TI Development of a PCR-Based Method for Detection of Delphinium Species in Poisoned Cattle SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Delphinium; PCR; diagnostic tool; rumen; poisoned ID TALL LARKSPUR DELPHINIUM; DAIRY-COWS; RUMEN; MAIZE; ALKALOIDS; DIGESTION; DNA; TRANSGENE; LIVESTOCK; PASSAGE AB Toxic plants such as Delphinium spp. (i.e., larkspur) are a significant cause of livestock losses worldwide. Correctly determining the causative agent responsible for the death of an animal, whether by disease, poisonous plant, or other means, is critical in developing strategies to prevent future losses. The objective of this study was to develop an alternative diagnostic tool to microscopy and analytical chemistry to determine whether a particular poisonous plant was ingested. Polymerase chain reaction (PCR) is a tool that may allow detection of the genetic material from a specific plant within a complex matrix such as rumen contents. A pair of oligonucleotide primers specific to Delphinium spp. (i.e., larkspur) was developed; using these primers, a PCR product was detected in samples from an in vivo, in vitro, and in vivo/in vitro coupled digestion of Delphinium occidentale. Lastly, larkspur was detected in a matrix of ruminal material where the amount of larkspur was far less than what one would expect to find in the rumen contents of a poisoned animal. The PCR-based technique holds promise to diagnose larkspur and perhaps other toxic plant caused losses. C1 [Cook, Daniel; Pfister, James A.; Constantino, John R.; Roper, Jessie M.; Gardner, Dale R.; Welch, Kevin D.; Hammond, Zachary J.; Green, Benedict T.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Cook, D (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov NR 31 TC 2 Z9 2 U1 2 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 4 PY 2015 VL 63 IS 4 BP 1220 EP 1225 DI 10.1021/jf5053496 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CA8AG UT WOS:000349138300021 ER PT J AU Dunn, KL Yang, LY Girard, A Bean, S Awika, JM AF Dunn, Kristen L. Yang, Liyi Girard, Audrey Bean, Scott Awika, Joseph M. TI Interaction of Sorghum Tannins with Wheat Proteins and Effect on in Vitro Starch and Protein Digestibility in a Baked Product Matrix SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE condensed tannins; gluten; starch digestibility; wheat; sorghum ID PERFORMANCE LIQUID-CHROMATOGRAPHY; RESISTANT STARCH; GLUTEN PROTEINS; PHENOLIC-ACIDS; BICOLOR; ANTIOXIDANT; QUALITY; GRAIN; SPECIFICITY; SEPARATION AB Carbohydrates contribute the most dietary calories, which makes starchy foods a logical target for modifying calorie intake. This study investigated the interaction of sorghum bran proanthocyanidins (PA) with proteins during wheat flour tortilla processing and impact on in vitro starch digestibility. Brans from wheat, white (low in phenols), brown (high PA), and black (high monomeric flavonoids) sorghum were used. Changes in phenolic profile, starch, and proteins were evaluated. Dough mixing drastically decreased extractable PA (61-72%) but not monomeric phenolics; higher MW PA decreased the most. The high PA bran dough produced the highest insoluble proteins (460 vs 330 mg/g protein for other sorghum brans) at 25% bakers substitution. The high PA bran tortillas also had higher slow digesting starch and lower rapidly digesting starch than all other bran treatments. Significant sorghum PA-gluten interactions occur during dough mixing that may slow starch digestibility in the baked products C1 [Dunn, Kristen L.; Yang, Liyi; Girard, Audrey; Awika, Joseph M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Girard, Audrey; Awika, Joseph M.] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA. [Bean, Scott] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Awika, JM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM jawika@ag.tamu.edu OI Bean, Scott/0000-0001-8678-8094 NR 46 TC 6 Z9 6 U1 7 U2 48 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD FEB 4 PY 2015 VL 63 IS 4 BP 1234 EP 1241 DI 10.1021/jf504112z PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CA8AG UT WOS:000349138300023 ER PT J AU Morris, DL Faaborg, J Washburn, BE Millspaugh, JJ AF Morris, Dana L. Faaborg, John Washburn, Brian E. Millspaugh, Joshua J. TI Predator-induced renesting and reproductive effort in indigo buntings: more work for less pay? SO CONSERVATION PHYSIOLOGY LA English DT Article DE Avian breeding biology; corticosterone; forest fragmentation; nest success; post-breeding; renesting ID INCREASED EGG-PRODUCTION; CLUTCH SIZE VARIATION; NON-BREEDING SEASON; NEST PREDATION; MIGRATORY BIRD; BODY CONDITION; CORTICOSTERONE LEVELS; ADRENOCORTICAL-RESPONSE; FOREST SONGBIRDS; HABITAT QUALITY AB Renesting after nest predation is ultimately an adaptive response to increase productivity in birds. However, renesting also increases reproductive effort to replace lost clutches. We investigated the consequences of this increased reproductive effort by determining whether renesting in female indigo buntings (Passerina cyanea) is associated with a decline in body condition (size-corrected mass) and haematocrit and an increase in stress hormones and whether renesting or maternal body condition is associated with a decline in productivity (clutch size, nestling body condition). Next, because a consequence of multiple renesting attempts is a prolonged breeding season and later timing, we predicted that a population of post-breeding females and juveniles would have lower body condition in fragmented forest than in contiguous forest owing to higher nest predation and frequency of renesting. Both forest types were settled by females of similar condition. Nest survival was lower in fragmented forest, where a higher proportion of females failed their first attempt and the breeding season was 2 weeks longer. Compared with females on their first attempt, renesting females had lower body condition and haematocrit and higher corticosterone concentrations. Lower maternal body condition was associated with higher concentrations of corticosterone, lower nestling body condition and smaller clutches. Clutch size was lower in renests and in fragmented forest. Nestling condition was lower in renests but did not vary greatly with forest type. Despite a prolonged breeding season in the fragmented forest, post-breeding females and hatch-year birds were in similar condition in both forest types. Our results suggest that the indirect effects of nest predation on maternal and offspring condition pose additional individual-level costs that have not been considered in the context of fragmentation studies. We discuss how predator-induced renesting could have additional demographic consequences by prolonging the breeding season and prompting seasonal interactions or carry-over effects that could impact populations. C1 [Morris, Dana L.; Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Morris, Dana L.] Cent Methodist Univ, Div Sci Math & Comp Sci, 411 Cent Methodist Sq, Fayette, MO 65248 USA. [Washburn, Brian E.; Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Washburn, Brian E.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. RP Morris, DL (reprint author), Cent Methodist Univ, Div Sci Math & Comp Sci, 411 Cent Methodist Sq, Fayette, MO 65248 USA. EM dlmorris@centralmethodist.edu FU National Center for Environmental Research (NCER) STAR Program, Environmental Protection Agency [U916163]; University of Missouri Conservation Biology Program Fellowship; US Forest Service North Central Research Center FX This work was supported by the National Center for Environmental Research (NCER) STAR Program, Environmental Protection Agency (grant number U916163), University of Missouri Conservation Biology Program Fellowship and the US Forest Service North Central Research Center. NR 97 TC 1 Z9 1 U1 2 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 2051-1434 J9 CONSERV PHYSIOL JI Conserv. Physiol. PD FEB 4 PY 2015 VL 3 AR cou063 DI 10.1093/conphys/cou063 PG 16 WC Biodiversity Conservation; Ecology; Environmental Sciences; Physiology SC Biodiversity & Conservation; Environmental Sciences & Ecology; Physiology GA DK8QU UT WOS:000375194100001 PM 27293684 ER PT J AU Bann, D Hire, D Manini, T Cooper, R Botoseneanu, A McDermott, MM Pahor, M Glynn, NW Fielding, R King, AC Church, T Ambrosius, WT Gill, T AF Bann, David Hire, Don Manini, Todd Cooper, Rachel Botoseneanu, Anda McDermott, Mary M. Pahor, Marco Glynn, Nancy W. Fielding, Roger King, Abby C. Church, Timothy Ambrosius, Walter T. Gill, Thomas CA Life Study Grp TI Light Intensity Physical Activity and Sedentary Behavior in Relation to Body Mass Index and Grip Strength in Older Adults: Cross-Sectional Findings from the Lifestyle Interventions and Independence for Elders (LIFE) Study SO PLOS ONE LA English DT Article ID BRITISH BIRTH COHORT; MUSCLE STRENGTH; AEROBIC EXERCISE; OBESITY; HEALTH; METAANALYSIS; TRIAL; AGE; ASSOCIATIONS; PERFORMANCE AB Background Identifying modifiable determinants of fat mass and muscle strength in older adults is important given their impact on physical functioning and health. Light intensity physical activity and sedentary behavior are potential determinants, but their relations to these outcomes are poorly understood. We evaluated associations of light intensity physical activity and sedentary time-assessed both objectively and by self-report-with body mass index (BMI) and grip strength in a large sample of older adults. Methods We used cross-sectional baseline data from 1130 participants of the Lifestyle Interventions and Independence for Elders (LIFE) study, a community-dwelling sample of relatively sedentary older adults (70-89 years) at heightened risk of mobility disability. Time spent sedentary and in light intensity activity were assessed using an accelerometer worn for 3-7 days (Actigraph GT3X) and by self-report. Associations between these exposures and measured BMI and grip strength were evaluated using linear regression. Results Greater time spent in light intensity activity and lower sedentary times were both associated with lower BMI. This was evident using objective measures of lower-light intensity, and both objective and self-reported measures of higher-light intensity activity. Time spent watching television was positively associated with BMI, while reading and computer use were not. Greater time spent in higher but not lower intensities of light activity (assessed objectively) was associated with greater grip strength in men but not women, while neither objectively assessed nor self-reported sedentary time was associated with grip strength. Conclusions In this cross-sectional study, greater time spent in light intensity activity and lower sedentary times were associated with lower BMI. These results are consistent with the hypothesis that replacing sedentary activities with light intensity activities could lead to lower BMI levels and obesity prevalence among the population of older adults. However, longitudinal and experimental studies are needed to strengthen causal inferences. C1 [Bann, David; Cooper, Rachel] UCL, MRC, Unit Lifelong Hlth & Ageing, London, England. [Hire, Don; Ambrosius, Walter T.] Wake Forest Sch Med, Div Publ Hlth Sci, Dept Biostat Sci, Winston Salem, NC USA. [Manini, Todd; Pahor, Marco] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL USA. [Botoseneanu, Anda] Univ Michigan, Dept Hlth Policy Studies, Ann Arbor, MI 48109 USA. [Botoseneanu, Anda] Univ Michigan, Inst Gerontol, Ann Arbor, MI 48109 USA. [McDermott, Mary M.] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. [Glynn, Nancy W.] Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USA. [Fielding, Roger] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [King, Abby C.] Stanford Univ, Sch Med, Stanford, CA 94305 USA. [Church, Timothy] Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Gill, Thomas] Yale Univ, Sch Med, New Haven, CT USA. RP Bann, D (reprint author), UCL, MRC, Unit Lifelong Hlth & Ageing, London, England. EM david.bann@ucl.ac.uk RI Buford, Thomas /B-1111-2011; OI Buford, Thomas /0000-0002-9541-4358; Marsiske, Michael/0000-0001-5973-2116; Glynn, Nancy/0000-0003-2265-0162; Cooper, Rachel/0000-0003-3370-5720; Bann, David/0000-0002-6454-626X FU Medical Research Council (UK) [MC_UU_12019/1, MC_UU_12019/4]; MRC Centenary Early Career Award; National Institutes of Health/National Institute on Aging Cooperative Agreement [U01AG22376]; National Heart, Lung and Blood Institute [3U01AG022376-05A2S]; Intramural Research Program, National Institute on Aging, NIH; Claude D. Pepper Older Americans Independence Centers at the University of Florida [1 P30 AG028740]; Wake Forest University [1 P30 AG21332]; Tufts University [1P30AG031679]; University of Pittsburgh [P30 AG024827]; Yale University [P30AG021342]; NIH/NCRR CTSA at Stanford University [UL1 RR025744]; Boston Rehabilitation Outcomes Center [1R24HD065688-01A1]; Academic Leadership Award [K07AG3587]; U.S. Department of Agriculture [58-1950-0-014] FX DB and RC are supported by the Medical Research Council (UK) (grant codes MC_UU_12019/1and MC_UU_ 12019/4). DB received an MRC Centenary Early Career Award to facilitate this work. The Lifestyle Interventions and Independence for Elders Study is funded by a National Institutes of Health/National Institute on Aging Cooperative Agreement #U01AG22376 and a supplement from the National Heart, Lung and Blood Institute 3U01AG022376-05A2S, and sponsored in part by the Intramural Research Program, National Institute on Aging, NIH. The research is partially supported by the Claude D. Pepper Older Americans Independence Centers at the University of Florida (1P30 AG028740), Wake Forest University (1 P30 AG21332), Tufts University (1P30AG031679), University of Pittsburgh (P30 AG024827), and Yale University (P30AG021342) and the NIH/NCRR CTSA at Stanford University (UL1 RR025744), Tufts University is also supported by the Boston Rehabilitation Outcomes Center (1R24HD065688-01A1). LIFE investigators are also partially supported by the following: Dr. Thomas Gill (Yale University) is the recipient of an Academic Leadership Award (K07AG3587) from the National Institute on Aging. Dr. Roger Fielding (Tufts University) is partially supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 53 TC 13 Z9 13 U1 3 U2 36 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD FEB 3 PY 2015 VL 10 IS 2 AR UNSP e0116058 DI 10.1371/journal.pone.0116058 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA3QZ UT WOS:000348822600018 PM 25647685 ER PT J AU McMahon, GM Hwang, SJ Tanner, RM Jacques, PF Selhub, J Muntner, P Fox, CS AF McMahon, Gearoid M. Hwang, Shih-Jen Tanner, Rikki M. Jacques, Paul F. Selhub, Jacob Muntner, Paul Fox, Caroline S. TI The association between vitamin B12, albuminuria and reduced kidney function: an observational cohort study SO BMC NEPHROLOGY LA English DT Article DE Homocysteine; Albuminuria; Vitamin B12; Reduced kidney function ID RANDOMIZED CONTROLLED-TRIAL; FOLATE; DEFICIENCY; PATHOPHYSIOLOGY; HEMODIALYSIS; METABOLISM; DISEASE; BLOOD AB Background: Variants in CUBN, the gene encoding cubilin, a proximal tubular transport protein, have been associated with albuminuria and vitamin B12 (B12) deficiency. We hypothesized that low levels of B12 would be associated with albuminuria in a population-based cohort. Methods: We analyzed participants from the Framingham Heart Study (n = 2965, mean age 58 years, 53% female) who provided samples for plasma B12. Logistic regression models adjusted for covariates including homocysteine were constructed to test the association between B12 and prevalent albuminuria (UACR >= 17 mg/g [ men] and >= 25 mg/g [women]) and reduced kidney function (defined as an eGFR < 60 ml/min/1.73 m(2), RKF). Because of a significant interaction between B12 and homocysteine in the prevalent RKF model (p = 0.005), the model was stratified by the median homocysteine levels. Logistic regression models were constructed to test the association between B12 and incident albuminuria and RKF. The results were replicated in 4445 participants from NHANES 2003-2004. Results: Baseline B12 levels ranged from 50-1690 pg/ml. Elevated B12 was associated with prevalent albuminuria (OR 1.44 per 1 SD increase, 95% CI 1.10-1.87) and RKF (OR 1.83, 95% CI 1.30-2.60). However after stratifying by median homocysteine levels, this relationship remained only in the higher homocysteine stratum. There was no association between B12 and incident albuminuria (OR 1.17, 95% CI 0.79 - 1.73) or RKF (OR 1.45, 95% CI 0.97 - 1.88). In the NHANES cohort, elevated B12 was associated with RKF after full covariate adjustment (OR 3.06, 95% CI 2.30-4.08). There was no association with albuminuria. Conclusion: In participants with high baseline homocysteine levels, increased plasma B12 was associated with RKF. C1 [McMahon, Gearoid M.; Hwang, Shih-Jen; Fox, Caroline S.] NHLBI, Framingham Heart Study, Framingham, MA 01702 USA. [McMahon, Gearoid M.; Hwang, Shih-Jen; Fox, Caroline S.] Ctr Populat Studies, Framingham, MA 01702 USA. [McMahon, Gearoid M.; Fox, Caroline S.] Brigham & Womens Hosp, Div Renal, Boston, MA 02115 USA. [McMahon, Gearoid M.; Fox, Caroline S.] Harvard Univ, Sch Med, Boston, MA USA. [Tanner, Rikki M.; Muntner, Paul] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Jacques, Paul F.; Selhub, Jacob] Tufts Univ, USDA, Human Nutr Ctr Aging, Boston, MA 02111 USA. [Fox, Caroline S.] Brigham & Womens Hosp, Div Endocrinol, Boston, MA 02115 USA. RP Fox, CS (reprint author), NHLBI, Framingham Heart Study, 73 Mt Wayte Ave,Suite 2, Framingham, MA 01702 USA. EM foxca@nhlbi.nih.gov OI McMahon, Gearoid/0000-0002-7723-2198 FU USDA [58-1950-0-014]; [N01-HC-25195] FX The Framingham Heart Study of the National Heart, Lung and Blood Institute is supported by contract N01-HC-25195. Drs. Selhub and Jacques are supported by USDA agreement No. 58-1950-0-014. The funding bodies played no role in the study design, analysis or interpretation of data, writing the manuscript or the decision to submit for publication. NR 25 TC 0 Z9 0 U1 0 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2369 J9 BMC NEPHROL JI BMC Nephrol. PD FEB 2 PY 2015 VL 16 AR 16 DI 10.1186/1471-2369-16-7 PG 8 WC Urology & Nephrology SC Urology & Nephrology GA CC4ID UT WOS:000350316000001 ER PT J AU Shi, M Dong, S Li, MT Yang, YY Stanley, D Chen, XX AF Shi, Min Dong, Shuai Li, Ming-tian Yang, Yan-yan Stanley, David Chen, Xue-xin TI The Endoparasitoid, Cotesia vestalis, Regulates Host Physiology by Reprogramming the Neuropeptide Transcriptional Network SO Scientific Reports LA English DT Article ID PLUTELLA-XYLOSTELLA; DIAMONDBACK MOTH; MANDUCA-SEXTA; BRACONID WASP; TOBACCO HORNWORM; PARASITISM; CONGREGATA; HORMONE; METAMORPHOSIS; ACCUMULATION AB Endoparasitoids develop inside another insect by regulating host immunity and development via maternal factors injected into hosts during oviposition. Prior results have provided insights into parasitism-induced immunosuppression, including the neuropeptide accumulation in parasitized insects. Nonetheless, our understanding of neuropeptide influence on host development and behavior is not yet complete. We posed the hypothesis that parasitization alters expression of genes encoding pro-neuropeptides and used larvae of Plutella xylostella and its endoparasitoid, Cotesia vestalis to test our hypothesis. We prepared transcriptomes from the larval P. xylostella brain-CC-CA complex and identified transcripts encoding 19 neuropeptides. All corresponding cDNAs were confirmed by RACE. Our results demonstrate that parasitism significantly down-regulated, or delayed, expression of genes encoding pro-neuropeptides within 48 h post-parasitization. Changing expression of these genes may account for the previously reported decreased feeding behavior, reduced growth rates and aborted development in the host larvae. In effect, parasitization may operate at the molecular level within the CNS to create global changes in larval host biology. The significance of our finding is that, in addition to the known effects on immunity, parasitoids influence host pro-neuropeptide gene transcription. This finding reveals a new mechanism operating in host-parasitoid relationships to the advantage of the parasitoid. C1 [Shi, Min; Dong, Shuai; Li, Ming-tian; Yang, Yan-yan; Chen, Xue-xin] Zhejiang Univ, Inst Insect Sci, Key Lab Agr Entomol, Minist Agr, Hangzhou 310058, Zhejiang, Peoples R China. [Stanley, David] USDA, ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Chen, XX (reprint author), Zhejiang Univ, Inst Insect Sci, Key Lab Agr Entomol, Minist Agr, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. EM xxchen@zju.edu.cn RI Chen, Xue-xin/D-1592-2010 OI Chen, Xue-xin/0000-0002-9109-8853 FU 973 Program [2013CB127600]; National Science Fund for Innovative Research Groups [31321063]; Zhejiang Science Fund for Distinguished Young Scholars [R3110049]; Fundamental Research Funds for the Central Universities FX We thank Dr. Kevin Clark for his help in reviewing an early draft of the manuscript. Funding for this study was provided jointly by the 973 Program (2013CB127600), the National Science Fund for Innovative Research Groups (31321063), the Zhejiang Science Fund for Distinguished Young Scholars (R3110049) and the Fundamental Research Funds for the Central Universities. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 37 TC 3 Z9 5 U1 6 U2 51 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD FEB 2 PY 2015 VL 5 AR 8173 DI 10.1038/srep08173 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA1KD UT WOS:000348670600002 PM 25640113 ER PT J AU Harrer, H Humpf, HU Voss, KA AF Harrer, Henning Humpf, Hans Ulrich Voss, Kenneth A. TI In vivo formation of N-acyl-fumonisin B-1 SO MYCOTOXIN RESEARCH LA English DT Article DE Fumonisin; Metabolism; Mycotoxin; Biodistribution; N-acyl-fumonisins ID NEURAL-TUBE DEFECTS; FUSARIUM-MONILIFORME; CERAMIDE SYNTHASE; HYDROLYZED FUMONISIN; B6C3F(1) MICE; TOXICITY; METABOLITES; RATS; LEUKOENCEPHALOMALACIA; CARCINOGENICITY AB Fumonisins are fungal toxins found in corn and in corn-based foods. Fumonisin B-1 (FB1) is the most common and is toxic to animals, causes cancer in rodents, and is a suspected risk factor for cancer and birth defects in humans. The hydrolyzed form of FB1 (HFB1) also occurs in foods and is metabolized by rats to compounds collectively known as N-acyl-HFB1 (also known as N-acyl-AP(1)). N-acyl-HFB1 is structurally similar to ceramides, metabolites which have important structural and signaling functions in cells. FB1 is N-acylated in vitro to ceramide-like metabolites which, like FB1, are cytotoxic. However, metabolism of FB1 and inhibition of ceramide synthase by its metabolites in vivo has not been demonstrated. Male rats were dosed ip with 0.5, 1, or 2 mg/kg body weight FB1 on five consecutive days and the liver and kidney thereafter processed for chemical analysis. N-acyl derivatives of fumonisin B-1 were identified for the first time in these principal target organs of FB1 in rats, at levels up to 0.4 nmol/g tissue using mass spectrometry. The N-acyl chain length of the metabolites varied in a tissue-dependent manner with C-16 derivatives predominating in the kidney and C-24 derivatives being prevalent in the liver. The toxicological significance of N-acyl-fumonisins is not known and warrants investigation. C1 [Harrer, Henning; Humpf, Hans Ulrich] Univ Munster, Inst Food Chem, D-48149 Munster, Germany. [Voss, Kenneth A.] USDA ARS, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. [Voss, Kenneth A.] ARS, Russell Res Ctr, USDA, Athens, GA USA. RP Humpf, HU (reprint author), Univ Munster, Inst Food Chem, D-48149 Munster, Germany. EM humpf@uni-muenster.de; Ken.Voss@ars.usda.gov NR 30 TC 6 Z9 6 U1 0 U2 4 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0178-7888 EI 1867-1632 J9 MYCOTOXIN RES JI Mycotoxin Res. PD FEB PY 2015 VL 31 IS 1 BP 33 EP 40 DI 10.1007/s12550-014-0211-5 PG 8 WC Mycology; Toxicology SC Mycology; Toxicology GA CS4XP UT WOS:000362079900005 PM 25326150 ER PT J AU Chung, SY Reed, S AF Chung, Si-Yin Reed, Shawndrika TI Making Peanut Allergens Indigestible: A Model System for Reducing or Preventing an Allergic Reaction SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 20-24, 2015 CL Houston, TX SP Amer Acad Allergy, Asthma & Immunol C1 [Chung, Si-Yin; Reed, Shawndrika] USDA ARS, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2015 VL 135 IS 2 SU S MA 103 BP AB33 EP AB33 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA CR2BN UT WOS:000361129600104 ER PT J AU Hurlburt, BK Cheng, HP Offermann, L Chruszcz, M Santos, AF Lack, G Maleki, SJ AF Hurlburt, Barry K. Cheng, Hsiaopo Offermann, Lesa Chruszcz, Maksymilian Santos, Alexandra F. Lack, Gideon Maleki, Soheila J. TI Epitope Mapping the Peanut Panallergen Ara h 8 SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 20-24, 2015 CL Houston, TX SP Amer Acad Allergy, Asthma & Immunol C1 [Hurlburt, Barry K.; Cheng, Hsiaopo; Maleki, Soheila J.] ARS, USDA, SRRC, New Orleans, LA USA. [Offermann, Lesa; Chruszcz, Maksymilian] Univ S Carolina, Columbia, SC 29208 USA. [Santos, Alexandra F.; Lack, Gideon] Kings Coll London, London WC2R 2LS, England. NR 0 TC 0 Z9 0 U1 0 U2 2 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2015 VL 135 IS 2 SU S MA 98 BP AB31 EP AB31 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA CR2BN UT WOS:000361129600099 ER PT J AU Maleki, SJ Bublin, M Chruszcz, M Charles, T Grimm, CC Cheng, HP Teuber, SS Hurlburt, BK Breiteneder, H Schein, C AF Maleki, Soheila J. Bublin, Merima Chruszcz, Maksymilian Charles, Tysheena Grimm, Casey C. Cheng, Hsiaopo Teuber, Suzanne S. Hurlburt, Barry K. Breiteneder, Heimo Schein, Catherine TI Identification of the Epitopes That Cause Cross-Reactions Between Peanuts and Tree Nuts SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 20-24, 2015 CL Houston, TX SP Amer Acad Allergy, Asthma & Immunol C1 [Maleki, Soheila J.] Tulane Univ, Sch Med, New Orleans, LA 70112 USA. [Bublin, Merima; Breiteneder, Heimo] Med Univ Vienna, Vienna, Austria. [Chruszcz, Maksymilian] Univ S Carolina, Columbia, SC 29208 USA. [Charles, Tysheena; Grimm, Casey C.; Cheng, Hsiaopo; Hurlburt, Barry K.] ARS, USDA, SRRC, New Orleans, LA USA. [Teuber, Suzanne S.] UC Davis Sch Med, Davis, CA USA. [Schein, Catherine] Fdn Appl Mol Evolut, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2015 VL 135 IS 2 SU S MA 100 BP AB32 EP AB32 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA CR2BN UT WOS:000361129600101 ER PT J AU Vargas, AM Grimm, CC Mattison, CP AF Vargas, Aurora M. Grimm, Casey C. Mattison, Christopher P. TI Expression, Purification, and Characterization of Recombinant Coptotermes Formosanus Tropomyosin SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Academy-of-Allergy-Asthma-and-Immunology (AAAAI) CY FEB 20-24, 2015 CL Houston, TX SP Amer Acad Allergy, Asthma & Immunol C1 [Vargas, Aurora M.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Grimm, Casey C.; Mattison, Christopher P.] USDA ARS SRRC, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 EI 1097-6825 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD FEB PY 2015 VL 135 IS 2 SU S MA 606 BP AB188 EP AB188 PG 1 WC Allergy; Immunology SC Allergy; Immunology GA CR2BN UT WOS:000361129600605 ER PT J AU Gergerich, RC Welliver, RA Gettys, S Osterbauer, NK Kamenidou, S Martin, RR Golino, DA Eastwell, K Fuchs, M Vidalakis, G Tzanetakis, IE AF Gergerich, Rose C. Welliver, Ruth A. Gettys, Sarah Osterbauer, Nancy K. Kamenidou, Sophia Martin, Robert R. Golino, Deborah A. Eastwell, Kenneth Fuchs, Marc Vidalakis, Georgios Tzanetakis, Ioannis E. TI Safeguarding Fruit Crops in the Age of Agricultural Globalization SO PLANT DISEASE LA English DT Article ID PLUM-POX-VIRUS; CITRUS-TRISTEZA-VIRUS; VEIN-ASSOCIATED-VIRUS; TOXOPTERA-CITRICIDA; GENOME SEQUENCE; PLANT VIROLOGY; UNITED-STATES; GRAPEVINE; DISEASE; PATHOGENS C1 [Gergerich, Rose C.; Tzanetakis, Ioannis E.] Univ Arkansas Syst, Div Agr, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Welliver, Ruth A.; Gettys, Sarah] Bur Plant Ind, Penn Dept Agr, Manila, Philippines. Oregon Dept Agr, Plant Hlth Program, Eugene, OR USA. [Kamenidou, Sophia; Vidalakis, Georgios] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Martin, Robert R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Golino, Deborah A.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Eastwell, Kenneth] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Fuchs, Marc] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Tzanetakis, IE (reprint author), Univ Arkansas Syst, Div Agr, Dept Plant Pathol, Fayetteville, AR 72701 USA. EM itzaneta@uark.edu FU APHIS-NCPN FX All participants acknowledge the funding received through APHIS-NCPN for the development of diagnostics and production of G1 plants and Section 10.201 and 10.007 of the U.S. Farm Bills of 2008 and 2014, respectively, for the development of certification standards for berries, citrus, fruit trees, and grapevine. A special note of thanks goes to Erich Rudyj, who has tirelessly served as the coordinator and supporter for the National Clean Plant Network since its inception in 2008. NR 110 TC 7 Z9 7 U1 2 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2015 VL 99 IS 2 BP 176 EP 187 DI 10.1094/PDIS-07-14-0762-FE PG 12 WC Plant Sciences SC Plant Sciences GA CQ8NQ UT WOS:000360865400001 ER PT J AU Demers, JE Crouch, JA Castlebury, LA AF Demers, Jill E. Crouch, Jo Anne Castlebury, Lisa A. TI A Multiplex Real-Time PCR Assay for the Detection of Puccinia horiana and P. chrysanthemi on Chrysanthemum SO PLANT DISEASE LA English DT Article ID X MORIFOLIUM; WHITE RUST AB Puccinia horiana, the cause of chrysanthemum white rust, is a regulated fungal plant pathogen in the United States, while P. chrysanthemi, the cause of chrysanthemum brown rust, is a widespread but less destructive pathogen. Accurate identification is essential to enforce quarantine measures, but the two species cannot be differentiated visually in the absence of mature spores or symptoms. A multiplex real-time PCR assay was developed to detect and discriminate between P chrysanthemi and P. horictna. Species-specific hydrolysis probes labeled with different fluorescent dyes were designed based on the rDNA internal transcribed spacer region. Seven fresh samples and 270 herbarium specimens of chrysanthemum rust were tested with the assay with results confirmed using spore morphology. P horiana and P. chtysanthemi were accurately detected from all fresh samples, and as little as 1 pg of template DNA was reproducibly detected. Of the herbarium specimens, 99% were positive for at least one species using the multiplex assay with 7% positive for both species. This multiplex assay can discriminate between P. chrysanthemi and P. horiana and provides an additional tool for identification of P horiana to ensure appropriate application of quarantine measures. C1 [Demers, Jill E.; Crouch, Jo Anne; Castlebury, Lisa A.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. RP Castlebury, LA (reprint author), ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM Lisa.Castlebury@ars.usda.gov OI Crouch, Jo Anne/0000-0001-6886-8090 FU USDA-APHIS Farm Bill [10201]; Friends of Agricultural Research - Beltsville student summer internship; USDA-ARS Headquarters Post-doctoral Fellowship FX We thank Bonnie To for help with processing the chrysanthemum rust samples and assay development, Tunesha Phipps for technical support, and the U.S. National Fungus Collections. This work was funded by USDA-APHIS 2013 Farm Bill 10201 and a 2013 Friends of Agricultural Research - Beltsville student summer internship. J.E.D. is supported by a 2012 USDA-ARS Headquarters Post-doctoral Fellowship to L.A.C. NR 25 TC 1 Z9 1 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2015 VL 99 IS 2 BP 195 EP 200 DI 10.1094/PDIS-06-14-0632-RE PG 6 WC Plant Sciences SC Plant Sciences GA CQ8NQ UT WOS:000360865400003 ER PT J AU Wiseman, MS Dugan, FM Kim, YK Xiao, CL AF Wiseman, M. S. Dugan, F. M. Kim, Y. K. Xiao, C. L. TI A Postharvest Fruit Rot of Apple Caused by Lambertella corni-maris in Washington State SO PLANT DISEASE LA English DT Article ID RIBOSOMAL DNA AB During surveys for postharvest diseases of apple conducted in Washington State, an unknown fruit rot was observed on stored apple fruit collected from commercial fruit packinghouses. This disease was present in 66 of the 179 grower lots sampled, accounting for an average 1 to 3% of the total decayed fruit sampled. The disease appeared to originate from infection of wounds on the fruit skin. Lesions were brown and decayed tissues were spongy. A Lambertella sp. was consistently isolated from the decayed fruit. Sequences of the fungus and those of Lambertella comi-maris in GenBank differed by 0 to 4 bp across the combined small ribosomal subunit + internal transcribed spacer + large ribosomal subunit regions with a maximum identity ranging from 99 to 100%. The fungus grew at 0 to 20 degrees C and formed apothecia on artificial media after 8 to 24 weeks. On potato dextrose agar under a 12-h photoperiod, apothecial dimensions were variable, ranging from 1 to 6 mm in diameter with stipes of 1 to 4 by 0.5 mm. Asci were 76 to 125 by 3.5 to 5.5 mu m, inoperculate, eight-spored, clavate, and narrowed at the base. Ascospores were aseptate, 7 to 10 by 2.5 to 4.5 mu m, uniseriate to biseriate, and orange-brown at maturity in the ascus. Colony characteristics included little or no aerial mycelium, dark-yellow to gray-black mycelium, gray-black pseudosclerotia, and yellow pigmentation in the agar. Morphological characteristics of the fungus overlapped with the description of L. corni-maris. 'Fuji' apple fruit that were wounded, inoculated with representative isolates, and incubated at 0 degrees C yielded the same symptoms as seen in packinghouses, and the fungus was reisolated from the diseased fruit. This is the first report of a fruit rot in stored apple caused by L. corni-maris in the United States. We propose Lambertella rot as the name of this disease. C1 [Wiseman, M. S.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Dugan, F. M.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Kim, Y. K.] Pace Int, Wapato, WA USA. [Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov NR 31 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2015 VL 99 IS 2 BP 201 EP 206 DI 10.1094/PDIS-03-14-0327-RE PG 6 WC Plant Sciences SC Plant Sciences GA CQ8NQ UT WOS:000360865400004 ER PT J AU de Carvalho, SA Nunes, WMD Belasque, J Machado, MA Croce, J Bock, CH Abdo, Z AF de Carvalho, Sergio Alves de Carvalho Nunes, William Mario Belasque, Jose, Jr. Machado, Marcos Antonio Croce-Filho, Jose Bock, Clive H. Abdo, Zaid TI Comparison of Resistance to Asiatic Citrus Canker Among Different Genotypes of Citrus in a Long-Term Canker-Resistance Field Screening Experiment in Brazil SO PLANT DISEASE LA English DT Article ID AXONOPODIS PV. CITRI; SUBSP CITRI; XANTHOMONAS-CAMPESTRIS; PHYLLOCNISTIS-CITRELLA; BACTERIUM-CITRI; SUSCEPTIBILITY; CULTIVARS; HYBRIDS; IDENTIFICATION; WINDBREAKS AB Asiatic citrus canker (ACC) is caused by Xanthomonas citri subsp. citri. The disease results in yield loss and renders fruit unfit for the fresh market. A 6-year study in Parana State, Brazil, was conducted to compare the susceptibility of 186 genotypes of citrus representing sweet orange (Citrus sinensis), mandarin (C. reticulata), Mediterranean mandarin (C. deliciosa), Clementine mandarin (C. clementina), Satsuma mandarin (C. unshiu), sour orange (C. aurantium), lemon (C. limon), sweet lime (C. aurantfolia), grapefruit (C. paradisi), and four hybrids (C. reticulata x Citrus sp., C. reticulata x C. paradisi, C. reticulata x C. sinensis, and C. unshiu x C. sinensis). Sweet orange (C. sinensis) was represented by the most genotypes (n = 141). The number of lesions per leaf was assessed 18 times from 2005 to 2010 (up to 4 times per year). The data were analyzed using mixed-model analysis of fixed and random effects, which showed a total of six resistance susceptibility groupings of species and hybrids. Based on species, the most resistant genotypes, on average, included Satsuma and lemon (mean lesions per leaf = 4.32 and 4.26, respectively), and the most susceptible genotypes were grapefruit and sweet lime, with 14.84 and 10.96 lesions per leaf, respectively. Genotypes of mandarin, sour orange, Mediterranean mandarin, and sweet orange had intermediate severity (5.48 to 9.56 lesions per leaf). The hybrids also showed a range of ACC severity but all were in the more resistant groupings (5.26 to 7.35 lesions per leaf). No genotype was immune to ACC. The most resistant genotype was 'Muscia' (C. reticulata) and the most susceptible was 'Valencia Frost' (C. sinensis) (1.86 and 14.78 lesions per leaf, respectively). Approximately one-sixth of the genotypes showed a negative relationship of mean lesions per leaf with time, suggesting increasing resistance as they aged, due to a reduction in either new flush or plant size and structure. These results of the relative susceptibility of different citrus genotypes can be used in future research and to assist in varietal selection or for breeding purposes both within Brazil and other regions where ACC is an issue. C1 [de Carvalho, Sergio Alves; Machado, Marcos Antonio] Ctr Citricultura Sylvio Moreira, IAC, BR-13490970 Cordeiropolis, SP, Brazil. [de Carvalho Nunes, William Mario] Univ Estadual Maringa, Nucl Pesquisa Biotecnol Aplicada, BR-87020900 Maringa, Parana, Brazil. [Belasque, Jose, Jr.] Univ Sao Paulo, Escola Super Agr Luiz Queiroz, Dept Fitopatol & Nematol, Piracicaba, SP, Brazil. [Croce-Filho, Jose] Secretaria Estado Agr & Abastecimento Paran, Maringa, Parana, Brazil. [Bock, Clive H.] USDA ARS SEFTNRL, Byron, GA 31008 USA. [Abdo, Zaid] USDA ARS SAA, Athens, GA 30605 USA. RP de Carvalho, SA (reprint author), Ctr Citricultura Sylvio Moreira, IAC, BR-13490970 Cordeiropolis, SP, Brazil. EM sergio@centrodecitricultura.br RI Belasque, Jose/B-7753-2015; Abdo, Zaid/E-5176-2017 OI Belasque, Jose/0000-0001-6059-2173; Abdo, Zaid/0000-0002-8272-7734 FU FAPESP [99/12594-3]; Fundecitrus [01/2007]; CNPq [480639/2008-6] FX This work was supported by a grant-in-aid for scientific research from FAPESP (99/12594-3), Fundecitrus (Call 01/2007), and CNPq (480639/2008-6). We thank J. A. Remolli for substantial assistance in plant maintenance and data gathering, J. J. Severino in preparation of X. citri subsp. citri inoculum, and A. M. O. Goncalves-Zuliani for reading the manuscript and making corrections and additions. NR 55 TC 3 Z9 3 U1 4 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2015 VL 99 IS 2 BP 207 EP 218 DI 10.1094/PDIS-04-14-0384-RE PG 12 WC Plant Sciences SC Plant Sciences GA CQ8NQ UT WOS:000360865400005 ER PT J AU Hammond, J Bampi, D Reinsel, MD AF Hammond, J. Bampi, D. Reinsel, M. D. TI First Report of Plantago asiatica mosaic virus in Imported Asiatic and Oriental Lilies (Lilium hybrids) in the United States. SO PLANT DISEASE LA English DT News Item C1 [Hammond, J.; Bampi, D.; Reinsel, M. D.] USDA ARS, Floral & Nursery Plants Res Unit, USNA, Beltsville, MD 20705 USA. RP Hammond, J (reprint author), USDA ARS, Floral & Nursery Plants Res Unit, USNA, Beltsville, MD 20705 USA. NR 4 TC 2 Z9 2 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD FEB PY 2015 VL 99 IS 2 BP 292 EP 292 DI 10.1094/PDIS-08-14-0792-PDN PG 1 WC Plant Sciences SC Plant Sciences GA CQ8NQ UT WOS:000360865400044 ER PT J AU McHugh, T Pan, ZL AF McHugh, Tara Pan, Zhongli TI Innovative Infrared Food Processing SO FOOD TECHNOLOGY LA English DT Article C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. [Pan, Zhongli] ARS, USDA, Hlth Proc Foods Res Unit, Albany, CA USA. [Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. EM tara.mchugh@ars.usda.gov; zhongli.pan@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD FEB PY 2015 VL 69 IS 2 BP 79 EP 81 PG 3 WC Food Science & Technology SC Food Science & Technology GA CB5EX UT WOS:000349651400017 ER PT J AU Baranowski, T AF Baranowski, Tom TI Games for Increasing Physical Activity: Mechanisms for Change SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material C1 [Baranowski, Tom] ARS, Pediat Behav Nutr & Phys Act, USDA, Childrens Nutr Res Ctr,Dept Pediat,Baylor Coll Me, Houston, TX USA. RP Baranowski, T (reprint author), Baylor Coll Med, Pediat Behav Nutr, Houston, TX 77030 USA. EM Thomas.Baranowski@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 NR 2 TC 5 Z9 5 U1 1 U2 1 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD FEB PY 2015 VL 4 IS 1 BP 1 EP 2 DI 10.1089/g4h.2014.0128 PG 2 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CJ9CD UT WOS:000355799100001 PM 26181672 ER PT J AU DeSmet, A Palmeira, A Beltran, A Brand, L Davies, VF Thompson, D AF DeSmet, Ann Palmeira, Antonio Beltran, Alicia Brand, Leah Davies, Vanessa Fernandes Thompson, Debbe TI The Yin and Yang of Formative Research in Designing Serious (Exer-)games SO GAMES FOR HEALTH JOURNAL LA English DT Article ID NUTRITION INTERVENTION; PARTICIPATORY RESEARCH; PHYSICAL-ACTIVITY; HEALTH; PROGRAM AB Despite its relevance, formative research on games may be an undervalued part of the game development process. At the 2014 International Society of Behavioral Nutrition and Physical Activity exergaming pre-conference satellite meeting, a roundtable discussion was held to assemble experiences and suggestions on enhancing the use of formative research in the development of active videogames (i.e., exergames). This article presents a summary of the concepts discussed. The discussants concluded that, although formative research may slightly expand the project timeline, the potential benefits include a game more in line with preferences of the intended users, with better operationalized theoretical constructs and broader stakeholder support, facilitating implementation and sustainability. It also improves the efficiency of other research parts because of a lower dropout rate of participants. Formative, qualitative research is thus a necessary complement to quantitative measurements of intervention outcomes, in a sort of Yin and Yang dynamic. An adapted version of formative research that casts a wider net may, however, be needed, involving both behavioral scientists and game developers, expanding the topics beyond the game's looks and soliciting the opinions of a larger group of stakeholders, such as implementers, gatekeepers, and funders. C1 [DeSmet, Ann] Univ Ghent, Dept Movement & Sport Sci, Fac Med & Hlth Sci, B-9000 Ghent, Belgium. [Palmeira, Antonio; Beltran, Alicia] Univ Lisbon, Fac Phys Educ & Sports, Lusophone Univ Humanities & Technol, P-1699 Lisbon, Portugal. [Palmeira, Antonio; Beltran, Alicia] Univ Lisbon, Fac Human Movement, CIPER, P-1699 Lisbon, Portugal. [Brand, Leah; Thompson, Debbe] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX USA. [Davies, Vanessa Fernandes] Univ Fed Santa Catarina, Ctr Hlth Sci, Grad Program Publ Hlth, BR-88040900 Florianopolis, SC, Brazil. RP DeSmet, A (reprint author), Univ Ghent, Dept Movement & Sport Sci, Watersportlaan 2, B-9000 Ghent, Belgium. EM Ann.DeSmet@UGent.be OI Brand, Leah/0000-0002-7465-0642; Palmeira, Antonio/0000-0001-6508-0599 NR 23 TC 6 Z9 6 U1 3 U2 8 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD FEB PY 2015 VL 4 IS 1 BP 63 EP 66 DI 10.1089/g4h.2014.0123 PG 4 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CJ9CD UT WOS:000355799100012 PM 26181683 ER PT J AU Wang, RRC Larson, SR Jensen, KB Bushman, BS DeHaan, LR Wang, SW Yan, XB AF Wang, Richard R. -C. Larson, Steve R. Jensen, Kevin B. Bushman, B. Shaun DeHaan, Lee R. Wang, Shuwen Yan, Xuebing TI Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species SO GENOME LA English DT Article DE genome constitution; molecular marker; phylogeny; Dasypyrum; Pseudoroegneria; Thinopyrum ID IN-SITU HYBRIDIZATION; THINOPYRUM-INTERMEDIUM; PHYLOGENETIC-RELATIONSHIPS; ELYTRIGIA-CAESPITOSA; TRIBE TRITICEAE; GENE DISCOVERY; NUCLEAR-DNA; DIVERSITY; TRANSFERABILITY; BESSARABICUM AB Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D. R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as to the origin of the different genomes in intermediate wheatgrass. Genotypic data obtained from newly developed EST-SSR primers derived from the putative progenitor diploid species Pseudoroegneria spicata (Pursh) A. Love (St genome), Thinopyrum bessarabicum (Savul. & Rayss) A. Love (J = J(b) = E-b), and Thinopyrum elongatum (Host) D. Dewey (E = J(e) = E-e) indicate that the V genome of Dasypyrum (Coss. & Durieu) T. Durand is not one of the three genomes in intermediate wheatgrass. Based on all available information in the literature and findings in this study, the genomic designation of intermediate wheatgrass should be changed to J(vs)J(r)St, where J(vs) and J(r) represent ancestral genomes of present-day J(b) of Th. bessarabicum and J(e) of Th. elongatum, with J(vs) being more ancient. Furthermore, the information suggests that the St genome in intermediate wheatgrass is most similar to the present-day St found in diploid species of Pseudoroegneria from Eurasia. C1 [Wang, Richard R. -C.; Larson, Steve R.; Jensen, Kevin B.; Bushman, B. Shaun; Yan, Xuebing] ARS, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. [DeHaan, Lee R.; Wang, Shuwen] Land Inst, Salina, KS 67401 USA. [Yan, Xuebing] Henan Agr Univ, Coll Anim Husb & Vet Sci, Zhengzhou 450002, Peoples R China. RP Wang, RRC (reprint author), ARS, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. EM Richard.Wang@ars.usda.gov NR 54 TC 6 Z9 6 U1 4 U2 12 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD FEB PY 2015 VL 58 IS 2 BP 63 EP 70 DI 10.1139/gen-2014-0186 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CM5KE UT WOS:000357725900001 PM 26000870 ER PT J AU Connor, EE Evock-Clover, CM Walker, MP Elsasser, TH Kahl, S AF Connor, E. E. Evock-Clover, C. M. Walker, M. P. Elsasser, T. H. Kahl, S. TI COMPARATIVE GUT PHYSIOLOGY SYMPOSIUM: Comparative physiology of glucagon-like peptide-2: Implications and applications for production and health of ruminants SO JOURNAL OF ANIMAL SCIENCE LA English DT Article; Proceedings Paper CT Comparative Gut Physiology Symposium held at the Joint Annual Meeting CY JUL 20-24, 2014 CL Kansas City, MO SP Pancosma DE cattle; glucagon-like peptide-2; gut health ID INTESTINAL BLOOD-FLOW; PROGLUCAGON-DERIVED PEPTIDES; PERFUSED PORCINE ILEUM; GASTRIC-ACID-SECRETION; NEONATAL PIGLETS; IN-VIVO; DIGESTIVE PHYSIOLOGY; LIPID ABSORPTION; ENTERIC NEURONS; GLP-2 RECEPTOR AB Glucagon-like peptide-2 (GLP-2) is a 33-amino acid peptide derived from proteolytic cleavage of proglucagon by prohormone convertase 1/3 in enteroendocrine L cells. Studies conducted in humans, in rodent models, and in vitro indicate that GLP-2 is secreted in response to the presence of molecules in the intestinal lumen, including fatty acids, carbohydrates, amino acids, and bile acids, which are detected by luminal chemosensors. The physiological actions of GLP-2 are mediated by its G protein-coupled receptor expressed primarily in the intestinal tract on enteric neurons, enteroendocrine cells, and myofibroblasts. The biological activity of GLP-2 is further regulated by dipeptidyl peptidase IV, which rapidly cleaves the N-terminus of GLP-2 that is responsible for GLP-2 receptor activation. Within the gut, GLP-2 increases nutrient absorption, crypt cell proliferation, and mesenteric blood flow and decreases gut permeability and motility, epithelial cell apoptosis, and inflammation. Outside the gut, GLP-2 reduces bone resorption, can suppress appetite, and is cytoprotective in the lung. Thus, GLP-2 has been studied intensively as a therapeutic to improve intestinal function of humans during parenteral nutrition and following small bowel resection and, more recently, as a treatment for osteoporosis and obesity-related disorders and to reduce cellular damage associated with inflammation of the gut and lungs. Recent studies demonstrate that many biological actions and properties of GLP-2 in ruminants are similar to those in nonruminants, including the potential to reduce intestinal nitro-oxidative stress in calves caused by parasitic diseases such as coccidiosis. Because of its beneficial impacts on nutrient absorption, gut healing, and normal gut development, GLP-2 therapy offers significant opportunities to improve calf health and production efficiency. However, GLP-2 therapies require an extended time course to achieve desired physiological responses, as well as daily administration because of the hormone's short half-life. Thus, practical means of administration and alternative strategies to enhance basal GLP-2 secretion (e. g., through specific feed additives), which are more likely to achieve consumer acceptance, are needed. Opportunities to address these challenges are discussed. C1 [Connor, E. E.; Evock-Clover, C. M.; Walker, M. P.; Elsasser, T. H.] USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Kahl, S.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. RP Connor, EE (reprint author), USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM erin.connor@ars.usda.gov NR 104 TC 7 Z9 7 U1 1 U2 16 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD FEB PY 2015 VL 93 IS 2 BP 492 EP 502 DI 10.2527/jas2014-8577 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA CL6OO UT WOS:000357086600004 PM 26020740 ER PT J AU Shoemaker, CA LaFrentz, BR AF Shoemaker, Craig A. LaFrentz, Benjamin R. TI Growth and survival of the fish pathogenic bacterium, Flavobacterium columnare, in tilapia mucus and porcine gastric mucin SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE Flavobacterium columnare; columnaris disease; mucus; fish; mucin ID CATFISH ICTALURUS-PUNCTATUS; CHANNEL CATFISH; FLEXIBACTER-COLUMNARIS; BINDING LECTIN; VIRULENCE; WATER; ADHESION; CHALLENGE; DISEASE; MODEL AB Flavobacterium columnare, an economically important Gram-negative bacterium of freshwater farmed fish, colonizes the skin and gills in the initial steps of pathogenesis. The surface of fish is coated with mucus made up of high molecular weight glycoproteins. Limited studies have described the ability of bacterial pathogens to grow in fish mucus. Our objective was to determine if F. columnare isolates could grow and survive in formulated water FW) containing autoclaved tilapia mucus or porcine gastric mucin. We demonstrated the ability of F. columnare genomovars I, II, II-B and III to replicate (2-3 logs) and survive (21 to > 100 days) in FWcontaining tilapia mucus. In a second experiment, genomovar I and II isolates were found to replicate in FW containing tilapia mucus or porcine mucin but not in FW only. From a practical standpoint, fish handling and/or hauling results in stress that leads to mucus sloughing often with subsequent F. columnare infection. Flavobacterium columnare utilizes fish mucus as a nutrient source, and studies are underway to determine if growth in mucus or mucin results in differential protein expression and/or increased virulence of F. columnare towards fish. C1 [Shoemaker, Craig A.; LaFrentz, Benjamin R.] ARS, USDA, Aquat Anim Hlth Res Unit, Auburn, AL 36832 USA. RP Shoemaker, CA (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM craig.shoemaker@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service, Current Research Information Systems Project [6420-32000-024-00D] FX This work was supported by the US Department of Agriculture, Agricultural Research Service, Current Research Information Systems Project no. 6420-32000-024-00D 'Integrated Aquatic Animal Health Strategies'. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 25 TC 0 Z9 0 U1 1 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0378-1097 EI 1574-6968 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD FEB PY 2015 VL 362 IS 4 AR fnu060 DI 10.1093/femsle/fnu060 PG 5 WC Microbiology SC Microbiology GA CL3XJ UT WOS:000356885000005 ER PT J AU Lebarbenchon, C Pedersen, JC Sreevatsan, S Ramey, AM Dugan, VG Halpin, RA Ferro, PJ Lupiani, B Enomoto, S Poulson, RL Smeltzer, M Cardona, CJ Tompkins, SM Wentworth, DE Stallknecht, DE Brown, JD AF Lebarbenchon, Camille Pedersen, Janice C. Sreevatsan, Srinand Ramey, Andrew M. Dugan, Vivien G. Halpin, Rebecca A. Ferro, Pamela J. Lupiani, Blanca Enomoto, Shinichiro Poulson, Rebecca L. Smeltzer, Martin Cardona, Carol J. Tompkins, S. Mark Wentworth, David E. Stallknecht, David E. Brown, Justin D. TI H7N9 influenza A virus in turkeys in Minnesota SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID INTERSPECIES TRANSMISSION; SEQUENCE-ANALYSIS; RECEPTOR-BINDING; HUMAN INFECTION; UNITED-STATES; WILD BIRDS; ORIGIN; EVOLUTION; PATTERNS; SUBTYPES AB Introductions of H7 influenza A virus (IAV) from wild birds into poultry have been documented worldwide, resulting in varying degrees of morbidity and mortality. H7 IAV infection in domestic poultry has served as a source of human infection and disease. We report the detection of H7N9 subtype IAVs in Minnesota (MN) turkey farms during 2009 and 2011. The full genome was sequenced from eight isolates as well as the haemagglutinin (HA) and neuraminidase (NA) gene segments of H7 and N9 virus subtypes for 108 isolates from North American wild birds between 1986 and 2012. Through maximum-likelihood and coalescent phylogenetic analyses, we identified the recent H7 and N9 IAV ancestors of the turkey-origin H7N9 IAVs, estimated the time and geographical origin of the ancestral viruses, and determined the relatedness between the 2009 and 2011 turkey-origin H7N9 IAVs. Analyses supported that the 2009 and 2011 viruses were distantly related genetically, suggesting that the two outbreaks arose from independent introduction events from wild birds. Our findings further supported that the 2011 MN turkey-origin H7N9 virus was closely related to H7N9 IAVs isolated in poultry in Nebraska during the same year. Although the precise origin of the wild-bird donor of the turkey-origin H7N9 IAVs could not be determined, our findings suggested that, for both the NA and HA gene segments, the MN turkey-origin H7N9 viruses were related to viruses circulating in wild birds between 2006 and 2011 in the Mississippi Flyway. C1 [Lebarbenchon, Camille; Ramey, Andrew M.; Poulson, Rebecca L.; Stallknecht, David E.; Brown, Justin D.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Pedersen, Janice C.; Smeltzer, Martin] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Sreevatsan, Srinand; Enomoto, Shinichiro] Univ Minnesota, Coll Vet Med, Vet Populat Med, St Paul, MN 55108 USA. [Ramey, Andrew M.] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Dugan, Vivien G.; Halpin, Rebecca A.; Wentworth, David E.] J Craig Venter Inst, Rockville, MD 20850 USA. [Ferro, Pamela J.; Lupiani, Blanca] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. [Cardona, Carol J.] Univ Minnesota, Dept Vet Biomed Sci, St Paul, MN 55108 USA. [Tompkins, S. Mark] Univ Georgia, Dept Infect Dis, Coll Vet Med, Athens, GA 30602 USA. RP Brown, JD (reprint author), Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. EM jubrown1@uga.edu RI Tompkins, Stephen/A-3317-2008; OI Tompkins, Stephen/0000-0002-1523-5588; Wentworth, David/0000-0002-5190-980X; Sreevatsan, Srinand/0000-0002-5162-2403 FU National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN266200700007C, HHSN272200900007C]; National Research Initiative of the US Department of Agriculture Cooperative State Research, Education, and Extension Service AICAP [2005-3560515388 (Z507201)] FX This work was funded by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services under contract nos HHSN266200700007C and HHSN272200900007C, and the National Research Initiative of the US Department of Agriculture Cooperative State Research, Education, and Extension Service AICAP grant 2005-3560515388 (Z507201). This manuscript was prepared whilst D. E. W. was employed at the J. Craig Venter Institute. The opinions expressed in this article are the authors' own and do not reflect the view of the Centers for Disease Control, the Department of Health and Human Services or the US Government. The funding agencies did not have any involvement in the study design, implementation or publishing of this study and the research presented herein represents the opinions of the authors, but not necessarily the opinions of the funding agencies. Any use of trade names is for descriptive purposes only and does not imply endorsement by the US Government. NR 31 TC 2 Z9 2 U1 0 U2 6 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD FEB PY 2015 VL 96 BP 269 EP 276 DI 10.1099/vir.0.067504-0 PN 2 PG 8 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CJ8WR UT WOS:000355784700005 PM 25351723 ER PT J AU Asao, S Bedoya-Arrieta, R Ryan, MG AF Asao, Shinichi Bedoya-Arrieta, Ricardo Ryan, Michael G. TI Variation in foliar respiration and wood CO2 efflux rates among species and canopy layers in a wet tropical forest SO TREE PHYSIOLOGY LA English DT Article DE autotrophic respiration; carbon fluxes and pools; ecophysiology; leaf dry mass per area; photosynthesis; rainforest; vertical gradient ID GROSS PRIMARY PRODUCTION; LEAF ECONOMICS SPECTRUM; CARBON USE EFFICIENCY; RAIN-FOREST; PLANT RESPIRATION; STEM RESPIRATION; DARK RESPIRATION; CLIMATE-CHANGE; TISSUE RESPIRATION; MAINTENANCE RESPIRATION AB As tropical forests respond to environmental change, autotrophic respiration may consume a greater proportion of carbon fixed in photosynthesis at the expense of growth, potentially turning the forests into a carbon source. Predicting such a response requires that we measure and place autotrophic respiration in a complete carbon budget, but extrapolating measurements of autotrophic respiration from chambers to ecosystem remains a challenge. High plant species diversity and complex canopy structure may cause respiration rates to vary and measurements that do not account for this complexity may introduce bias in extrapolation more detrimental than uncertainty. Using experimental plantations of four native tree species with two canopy layers, we examined whether species and canopy layers vary in foliar respiration and wood CO2 efflux and whether the variation relates to commonly used scalars of mass, nitrogen (N), photosynthetic capacity and wood size. Foliar respiration rate varied threefold between canopy layers, similar to 0.74 mu mol m(-2) s(-1) in the overstory and similar to 0.25 mu mol m(-2) s(-1) in the understory, but little among species. Leaf mass per area, N and photosynthetic capacity explained some of the variation, but height explained more. Chamber measurements of foliar respiration thus can be extrapolated to the canopy with rates and leaf area specific to each canopy layer or height class. If area-based rates are sampled across canopy layers, the area-based rate may be regressed against leaf mass per area to derive the slope (per mass rate) to extrapolate to the canopy using the total leaf mass. Wood CO2 efflux varied 1.0-1.6 mu mol m(-2) s(-1) for overstory trees and 0.6-0.9 mu mol m(-2) s(-1) for understory species. The variation in wood CO2 efflux rate was mostly related to wood size, and little to species, canopy layer or height. Mean wood CO2 efflux rate per surface area, derived by regressing CO2 efflux per mass against the ratio of surface area to mass, can be extrapolated to the stand using total wood surface area. The temperature response of foliar respiration was similar for three of the four species, and wood CO2 efflux was similar between wet and dry seasons. For these species and this forest, vertical sampling may yield more accurate estimates than would temporal sampling. C1 [Asao, Shinichi] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Asao, Shinichi; Ryan, Michael G.] Colorado State Univ, Nat Resources Ecol Lab, Ft Collins, CO 80523 USA. [Bedoya-Arrieta, Ricardo] Org Trop Studies, La Selva Biol Stn, Puerto Viejo de Sarapiqui, Costa Rica. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Asao, S (reprint author), Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. EM shinichi.asao@colostate.edu RI Ryan, Michael/A-9805-2008; Asao, Shinichi/R-9514-2016 OI Ryan, Michael/0000-0002-2500-6738; Asao, Shinichi/0000-0002-0334-5464 FU National Science Foundation [EF-0236502, DEB-0703561]; Australia's Commonwealth Scientific and Industrial Research Organization (CSIRO) McMaster Fellowship FX This research was supported by National Science Foundation grants EF-0236502 and DEB-0703561. M.G.R. was partially supported by Australia's Commonwealth Scientific and Industrial Research Organization (CSIRO) McMaster Fellowship. NR 93 TC 3 Z9 3 U1 11 U2 35 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD FEB PY 2015 VL 35 IS 2 BP 148 EP 159 DI 10.1093/treephys/tpu107 PG 12 WC Forestry SC Forestry GA CI5EQ UT WOS:000354776700005 PM 25597756 ER PT J AU Bagley, J Rosenthal, DM Ruiz-Vera, UM Siebers, MH Kumar, P Ort, DR Bernacchi, CJ AF Bagley, Justin Rosenthal, David M. Ruiz-Vera, Ursula M. Siebers, Matthew H. Kumar, Praveen Ort, Donald R. Bernacchi, Carl J. TI The influence of photosynthetic acclimation to rising CO2 and warmer temperatures on leaf and canopy photosynthesis models SO GLOBAL BIOGEOCHEMICAL CYCLES LA English DT Article ID SOYBEAN GLYCINE-MAX; ELEVATED CO2; STOMATAL CONDUCTANCE; ATMOSPHERIC CO2; GAS-EXCHANGE; LIMITED PHOTOSYNTHESIS; RESPONSE FUNCTIONS; BIOCHEMICAL-MODEL; PARTIAL-PRESSURE; INFRARED HEATER AB There is an increasing necessity to understand how climate change factors, particularly increasing atmospheric concentrations of CO2 ([CO2]) and rising temperature, will influence photosynthetic carbon assimilation (A). Based on theory, an increased [CO2] concomitant with a rise in temperature will increase A in C3 plants beyond that of an increase in [CO2] alone. However, uncertainty surrounding the acclimation response of key photosynthetic parameters to these changes can influence this response. In this work, the acclimation responses of C3 photosynthesis for soybean measured at the SoyFACE Temperature by Free-Air CO2 Enrichment experiment are incorporated in a leaf biochemical and canopy photosynthesis model. The two key parameters used as model inputs, the maximum velocity for carboxylation (V-c,V-max) and maximum rate of electron transport (J(max)), were measured in a full factorial [CO2] by temperature experiment over two growing seasons and applied in leaf-and canopy-scale models to (1) reassess the theory of combined increases in [CO2] and temperature on A, (2) determine the role of photosynthetic acclimation to increased growth [CO2] and/or temperature in leaf and canopy predictions of A for these treatments, and (3) assess the diurnal and seasonal differences in leaf-and canopy-scale A associated with the imposed treatments. The results demonstrate that the theory behind combined increases in [CO2] and temperature is sound; however, incorporatingmore recent parameterizations into the photosynthesis model predicts greater increases in A when [CO2] and temperature are increased together. Photosynthetic acclimation is shown to decrease leaf-level A for all treatments; however, in elevated [CO2] the impact of acclimation does not result in any appreciable loss in photosynthetic potential at the canopy scale. In this analysis, neglecting photosynthetic acclimation in heated treatments, with or without concomitant rise in [CO2], leads to modeled overestimates of carbon gain for soybean under future predicted conditions. C1 [Bagley, Justin; Ort, Donald R.; Bernacchi, Carl J.] Univ Illinois, Genom Ecol Global Change & Energy Biosci Inst, Inst Genom Biol, Urbana, IL 61801 USA. [Rosenthal, David M.] Ohio Univ, Dept Environm & Plant Biol, Athens, OH 45701 USA. [Ruiz-Vera, Ursula M.; Siebers, Matthew H.; Ort, Donald R.; Bernacchi, Carl J.] Univ Illinois, Dept Plant Biol, Urbana, IL USA. [Kumar, Praveen] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL USA. [Ort, Donald R.; Bernacchi, Carl J.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL USA. RP Bernacchi, CJ (reprint author), Univ Illinois, Genom Ecol Global Change & Energy Biosci Inst, Inst Genom Biol, Urbana, IL 61801 USA. EM bernacch@illinois.edu RI Kumar, Praveen/D-2036-2010; Bagley, Justin/I-8019-2016 OI Kumar, Praveen/0000-0002-4787-0308; Bagley, Justin/0000-0003-4534-431X NR 62 TC 8 Z9 8 U1 15 U2 46 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0886-6236 EI 1944-9224 J9 GLOBAL BIOGEOCHEM CY JI Glob. Biogeochem. Cycle PD FEB PY 2015 VL 29 IS 2 BP 194 EP 206 DI 10.1002/2014GB004848 PG 13 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA CH9UF UT WOS:000354381200006 ER PT J AU Schennink, A Trott, JF Manjarin, R Lemay, DG Freking, BA Hovey, RC AF Schennink, Anke Trott, Josephine F. Manjarin, Rodrigo Lemay, Danielle G. Freking, Bradley A. Hovey, Russell C. TI Comparative genomics reveals tissue-specific regulation of prolactin receptor gene expression SO JOURNAL OF MOLECULAR ENDOCRINOLOGY LA English DT Article DE prolactin; receptor; kidney; transcription factors; gene regulation ID MULTIPLE 1ST EXONS; PORCINE PROLACTIN; PERIPARTUM CARDIOMYOPATHY; CARDIOVASCULAR CHANGES; CALCIUM-TRANSPORT; CHOROID-PLEXUS; MAMMARY-GLAND; MESSENGER-RNA; SHORT-FORM; LACTATION AB Prolactin (PRL), acting via the PRL receptor (PRLR), controls hundreds of biological processes across a range of species. Endocrine PRL elicits well-documented effects on target tissues such as the mammary glands and reproductive organs in addition to coordinating whole-body homeostasis during states such as lactation or adaptive responses to the environment. While changes in PRLR expression likely facilitates these tissue-specific responses to circulating PRL, the mechanisms regulating this regulation in non-rodent species has received limited attention. We performed a wide-scale analysis of PRLR 50 transcriptional regulation in pig tissues. Apart from the abundantly expressed and widely conserved exon 1, we identified alternative splicing of transcripts from an additional nine first exons of the porcine PRLR (pPRLR) gene. Notably, exon 1.5 transcripts were expressed most abundantly in the heart, while expression of exon 1.3-containing transcripts was greatest in the kidneys and small intestine. Expression of exon 1.3 mRNAs within the kidneys was most abundant in the renal cortex, and increased during gestation. A comparative analysis revealed a human homologue to exon 1.3, hE1(N2), which was also principally transcribed in the kidneys and small intestines, and an exon hE1(N3) was only expressed in the kidneys of humans. Promoter alignment revealed conserved motifs within the proximal promoter upstream of exon 1.3, including putative binding sites for hepatocyte nuclear factor-1 and Sp1. Together, these results highlight the diverse, conserved and tissue-specific regulation of PRLR expression in the targets for PRL, which may function to coordinate complex physiological states such as lactation and osmoregulation. C1 [Schennink, Anke; Trott, Josephine F.; Hovey, Russell C.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Lemay, Danielle G.] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Manjarin, Rodrigo] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. [Freking, Bradley A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Trott, JF (reprint author), Univ Calif Davis, Dept Anim Sci, 2335 Meyer Hall,One Shields Ave, Davis, CA 95616 USA. EM jftrott@ucdavis.edu FU National Research Initiative Competitive from the USDA National Institute for Food and Agriculture [2008-35206-18895] FX This project was supported by National Research Initiative Competitive grant number 2008-35206-18895 from the USDA National Institute for Food and Agriculture. NR 55 TC 3 Z9 3 U1 2 U2 16 PU BIOSCIENTIFICA LTD PI BRISTOL PA EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT, ENGLAND SN 0952-5041 J9 J MOL ENDOCRINOL JI J. Mol. Endocrinol. PD FEB PY 2015 VL 54 IS 1 BP 1 EP 15 DI 10.1530/JME-14-0212 PG 15 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CI3EE UT WOS:000354629600003 PM 25358647 ER PT J AU Roberts, SL Kelt, DA van Wagtendonk, JW Miles, AK Meyer, MD AF Roberts, Susan L. Kelt, Douglas A. van Wagtendonk, Jan W. Miles, A. Keith Meyer, Marc D. TI Effects of fire on small mammal communities in frequent-fire forests in California SO JOURNAL OF MAMMALOGY LA English DT Article DE fire effects; frequent-fire forests; nonparametric multiplicative modeling; reintroduced fire; small mammal community; species diversity ID NORTHERN FLYING SQUIRRELS; YOSEMITE-NATIONAL-PARK; MIXED-CONIFER FOREST; SIERRA-NEVADA; NORTHEASTERN CALIFORNIA; GLAUCOMYS-SABRINUS; SEVERITY; HABITAT; USA; ABUNDANCE AB Fire is a natural, dynamic process that is integral to maintaining ecosystem function. The reintroduction of fire (e.g., prescribed fire, managed wildfire) is a critical management tool for protecting many frequent-fire forests against stand-replacing fires while restoring an essential ecological process. Understanding the effects of fire on forests and wildlife communities is important in natural resource planning efforts. Small mammals are key components of forest food webs and essential to ecosystem function. To investigate the relationship of fire to small mammal assemblages, we live trapped small mammals in 10 burned and 10 unburned forests over 2 years in the central Sierra Nevada, California. Small mammal abundance was higher in unburned forests, largely reflecting the greater proportion of closed-canopy species such as Glaucomys sabrinus in unburned forests. The most abundant species across the entire study area was the highly adaptable generalist species, Peromyscus maniculatus. Species diversity was similar between burned and unburned forests, but burned forests were characterized by greater habitat heterogeneity and higher small mammal species evenness. The use and reintroduction of fire to maintain a matrix of burn severities, including large patches of unburned refugia, creates a heterogeneous and resilient landscape that allows for fire-sensitive species to proliferate and, as such, may help maintain key ecological functions and diverse small mammal assemblages. C1 [Roberts, Susan L.] US Geol Survey, Western Ecol Res Ctr, Yosemite Field Stn, Yosemite, CA 95389 USA. [Roberts, Susan L.; Kelt, Douglas A.] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [van Wagtendonk, Jan W.] US Geol Survey, Western Ecol Res Ctr, Yosemite Field Stn, El Portal, CA 95318 USA. [Miles, A. Keith] US Geol Survey, Western Ecol Res Ctr, Sacramento, CA 95819 USA. [Meyer, Marc D.] US Forest Serv, USDA, Clovis, CA 93611 USA. RP Roberts, SL (reprint author), US Geol Survey, Western Ecol Res Ctr, Yosemite Field Stn, POB 2163, Yosemite, CA 95389 USA. EM sroberts.sierra@gmail.com FU Yosemite National Park Fee Program; US Geological Survey Terrestrial, Freshwater, and Marine Ecosystems Program; University of California, Merced FX We thank Yosemite National Park Fee Program and the US Geological Survey Terrestrial, Freshwater, and Marine Ecosystems Program for funding the project. We also thank S. Thompson, E. Berlow, and the University of California, Merced, for additional support. J. Miller and A. Thode graciously provided the classified satellite imagery. We are grateful to R. Anthony for loaning us traps, S. Eyes for her analytical help, and to J. Yee for her review of the analyses. The project would not have been possible without the dedicated efforts of the field crews. The suggestions from 2 anonymous reviewers greatly improved this manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the United States Government. NR 67 TC 6 Z9 6 U1 11 U2 47 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2372 EI 1545-1542 J9 J MAMMAL JI J. Mammal. PD FEB PY 2015 VL 96 IS 1 BP 107 EP 119 DI 10.1093/jmammal/gyu011 PG 13 WC Zoology SC Zoology GA CG5LV UT WOS:000353334300014 ER PT J AU Aziz, I Bangash, N Mahmood, T Islam, KR AF Aziz, I. Bangash, N. Mahmood, T. Islam, K. R. TI IMPACT OF NO-TILL AND CONVENTIONAL TILLAGE PRACTICES ON SOIL CHEMICAL PROPERTIES SO PAKISTAN JOURNAL OF BOTANY LA English DT Article DE Tillage systems; Nitrogen; Total carbon; Active carbon; Passive organic carbon; Crop rotations ID ORGANIC-MATTER FRACTIONS; COASTAL-PLAIN SOILS; CARBON SEQUESTRATION; LAND-USE; NUTRIENT DISTRIBUTION; MICROBIAL BIOMASS; NITROGEN STORAGE; SYSTEMS; MANAGEMENT; AGGREGATION AB There is a global concern about progressive increase in the emission of greenhouse gases especially atmosphere CO2. An increasing awareness about environmental pollution by CO2 emission has led to recognition of the need to enhance soil C sequestration through sustainable agricultural management practices. Conservation management systems such as no-till (NT) with appropriate crop rotation have been reported to increase soil organic C content by creating less disturbed environment. The present study was conducted on Vanmeter farm of The Ohio State University South Centers at Piketon Ohio, USA to estimate the effect of different tillage practices with different cropping system on soil chemical properties. Tillage treatments were comprised of conventional tillage (CT) and No-till (NT). These treatments were applied under continuous corn (CC), corn-soybean (CS) and corn-soybean-wheat-cowpea (CSW) cropping system following randomized complete block design. No-till treatment showed significant increase in total C (30%), active C (10%), and passive salt extractable (18%) and microwave extractable C (8%) and total nitrogen (15%) compared to conventional tillage practices. Total nitrogen increased significantly 23 % in NT over time. Maximum effect of no-till was observed under corn-soybean-wheat-cowpea crop rotation. These findings illustrated that no-till practice could be useful for improving soil chemical properties. C1 [Aziz, I.] PMAS Arid Agr Univ, Dept Agron, Rawalpindi, Pakistan. [Bangash, N.; Mahmood, T.] PMAS Arid Agr Univ, Dept Environm Sci, Rawalpindi, Pakistan. [Islam, K. R.] Ohio State Univ South Ctr, Soil & Water Resources, Piketon, OH USA. [Islam, K. R.] USDA ARS, Soil Drainage Res, Columbus, OH USA. RP Aziz, I (reprint author), PMAS Arid Agr Univ, Dept Agron, Rawalpindi, Pakistan. EM irfaz15@yahoo.com NR 68 TC 2 Z9 2 U1 0 U2 7 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 EI 2070-3368 J9 PAK J BOT JI Pak. J. Bot. PD FEB PY 2015 VL 47 IS 1 BP 297 EP 303 PG 7 WC Plant Sciences SC Plant Sciences GA CG1JT UT WOS:000353029600042 ER PT J AU Rosmana, A Samuels, GJ Ismaiel, A Ibrahim, ES Chaverri, P Herawati, Y Asman, A AF Rosmana, Ade Samuels, Gary J. Ismaiel, Adnan Ibrahim, Eli Surya Chaverri, Priscila Herawati, Yustina Asman, Asman TI Trichoderma asperellum: A Dominant Endophyte Species in Cacao Grown in Sulawesi with Potential for Controlling Vascular Streak Dieback Disease SO TROPICAL PLANT PATHOLOGY LA English DT Article DE Ceratobasidium theobromae; Theobroma cacao; Fungi isolates; Seedling; Shoot graft ID BIOLOGICAL-CONTROL; THEOBROMA-CACAO; SOUTHEAST-ASIA; SP NOV.; PATHOGEN; MYCOPARASITISM AB Endophytic strains of Trichoderma species can be used as an alternative to chemicals to control vascular streak dieback (VSD) disease of cacao. Of 21 Trichoderma isolated from Theobroma cacao (cacao) in Indonesia, 19 were identified as Trichoderma asperellum. Four isolates of this species (ART-4, ART-5, ART-6 and ART-8) were reintroduced into young cocoa seedlings by root inoculation and after 4 weeks all were recovered from roots and stems, while ART-4 and ART-5 were recovered from leaves as well. Spraying seedlings pre-inoculated with T. asperellum ART-4, ART-5 and ART-6 with mycelium of the VSD pathogen Ceratobasidium theobromae resulted in no apparent VSD symptoms on the leaves. Those seedlings pre-inoculated with ART-8 showed 8.9 % incidence of VSD symptoms on the leaves when compared to a 20.4 % incidence of VSD on positive control leaves, 12 weeks after inoculation. The same isolates were also reintroduced into 3-month-old cocoa seedlings via the connecting site following shoot grafting, and after 12 weeks all isolates were recovered from stem and leaves. Seedlings grafted with buds infected by VSD and treated by ART-4 showed no VSD symptoms on their leaves 12 weeks after grafting and inoculation. However, those treated with ART-5, ART-6 and ART-8 showed 33.3 %, 50.0 % and 56.0 % incidence of VSD symptoms on their leaves, respectively, compared to an 88.9 % incidence of VSD on positive control leaves. Therefore, the study results demonstrates for the first time the potential of T. asperellum isolates to control VSD disease on cacao. C1 [Rosmana, Ade; Ibrahim, Eli Surya; Herawati, Yustina; Asman, Asman] Hasanuddin Univ, Fac Agr, Cocoa Res Grp, Makassar 90245, Indonesia. [Samuels, Gary J.; Ismaiel, Adnan] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Chaverri, Priscila] Univ Maryland, Dept Plant Sci & Landscape Architecture, Beltsville, MD 20705 USA. RP Rosmana, A (reprint author), Hasanuddin Univ, Fac Agr, Cocoa Res Grp, Makassar 90245, Indonesia. EM aderosmana65@gmail.com FU Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture through KKP3T research grant; Directorate General of Higher Education, Ministry of Education and Culture through PAR-C research grant FX The authors are grateful to the Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture and Directorate General of Higher Education, Ministry of Education and Culture who have supported this research through KKP3T and PAR-C research grants, respectively. Also, we would like to thank the University of Maryland and the United States Department of Agriculture, Beltsville, MD, USA, for giving permission to complete the research in their laboratories. NR 30 TC 2 Z9 2 U1 3 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1983-2052 J9 TROP PLANT PATHOL JI Trop. Plant Pathol. PD FEB PY 2015 VL 40 IS 1 BP 19 EP 25 DI 10.1007/s40858-015-0004-1 PG 7 WC Plant Sciences SC Plant Sciences GA CG3XW UT WOS:000353214000004 ER PT J AU Zhao, TJ Shi, JC Bindlish, R Jackson, TJ Kerr, YH Cosh, MH Cui, Q Li, YQ Xiong, C Che, T AF Zhao, Tianjie Shi, Jiancheng Bindlish, Rajat Jackson, Thomas J. Kerr, Yann H. Cosh, Michael H. Cui, Qian Li, Yunqing Xiong, Chuan Che, Tao TI Refinement of SMOS Multiangular Brightness Temperature Toward Soil Moisture Retrieval and Its Analysis Over Reference Targets SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Brightness temperature; intercomparison; soil moisture; soil moisture ocean salinity (SMOS); WindSat ID MICROWAVE EMISSION; L-BAND; MODEL; VALIDATION; PRODUCTS; MISSION; RADIOMETRY; SURFACE; CALIBRATION; ALGORITHM AB Soil moisture ocean salinity (SMOS) mission has been providing L-band multiangular brightness temperature observations at a global scale since its launch in November 2009 and has performed well in the retrieval of soil moisture. The multiple incidence angle observations also allow for the retrieval of additional parameters beyond soil moisture, but these are not obtained at fixed values and the resolution and accuracy change with the grid locations over SMOS snapshot images. Radio-frequency interference (RFI) issues and aliasing at lower look angles increase the uncertainty of observations and thereby affect the soil moisture retrieval that utilizes observations at specific angles. In this study, we proposed a two-step regression approach that uses a mixed objective function based on SMOS L1c data products to refine characteristics of multiangular observations. The approach was found to be robust by validation using simulations from a radiative transfer model, and valuable in improving soil moisture estimates from SMOS. In addition, refined brightness temperatures were analyzed over three external targets: Antarctic ice sheet, Amazon rainforest, and Sahara desert, by comparing with WindSat observations. These results provide insights for selecting and utilizing external targets as part of the upcoming soil moisture active passive (SMAP) mission. C1 [Zhao, Tianjie; Shi, Jiancheng; Cui, Qian; Li, Yunqing; Xiong, Chuan] Chinese Acad Sci, Inst Remote Sensing & Digital Earth RADI, State Key Lab Remote Sensing Sci, Beijing 100101, Peoples R China. [Zhao, Tianjie; Shi, Jiancheng; Cui, Qian; Li, Yunqing; Xiong, Chuan] Beijing Normal Univ, Beijing 100101, Peoples R China. [Zhao, Tianjie; Shi, Jiancheng; Cui, Qian; Li, Yunqing; Xiong, Chuan] Joint Ctr Global Change Studies, Beijing 100875, Peoples R China. [Bindlish, Rajat; Jackson, Thomas J.; Cosh, Michael H.] ARS, HRSL, USDA, Beltsville, MD USA. [Kerr, Yann H.] Ctr EtudesSpatiales Biosphere CESBIO, F-31401 Toulouse, France. [Che, Tao] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China. RP Zhao, TJ (reprint author), Chinese Acad Sci, Inst Remote Sensing & Digital Earth RADI, State Key Lab Remote Sensing Sci, Beijing 100101, Peoples R China. EM zhaotj@radi.ac.cn; shijc@radi.ac.cn; rajat.bindlish@ars.usda.gov; tom.jackson@ars.usda.gov; yann.kerr@cesbio.cnes.fr; chetao@lzb.ac.cn RI Che, Tao/K-7136-2013; westgis.CAREERI, SCI paper/O-2255-2013; OI Che, Tao/0000-0001-6848-7271; westgis.CAREERI, SCI paper/0000-0001-5298-1494; Cosh, Michael/0000-0003-4776-1918 FU National Natural Science Foundation of China [41301396]; CAS/SAFEA International Partnership Program for Creative Research Teams [KZZD-EW-TZ-09]; French National Center for Scientific Research (CNRS); National Natural Science Foundation of China (NSFC) FX This work was supported in part by the National Natural Science Foundation of China under Grant 41301396 and in part by CAS/SAFEA International Partnership Program for Creative Research Teams (KZZD-EW-TZ-09) and the cooperation project between the French National Center for Scientific Research (CNRS) and National Natural Science Foundation of China (NSFC). NR 43 TC 9 Z9 9 U1 4 U2 22 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD FEB PY 2015 VL 8 IS 2 BP 589 EP 603 DI 10.1109/JSTARS.2014.2336664 PG 15 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA CF1AY UT WOS:000352277100014 ER PT J AU Spindel, J Begum, H Akdemir, D Virk, P Collard, B Redona, E Atlin, G Jannink, JL McCouch, SR AF Spindel, Jennifer Begum, Hasina Akdemir, Deniz Virk, Parminder Collard, Bertrand Redona, Edilberto Atlin, Gary Jannink, Jean-Luc McCouch, Susan R. TI Genomic Selection and Association Mapping in Rice (Oryza sativa): Effect of Trait Genetic Architecture, Training Population Composition, Marker Number and Statistical Model on Accuracy of Rice Genomic Selection in Elite, Tropical Rice Breeding Lines SO PLOS GENETICS LA English DT Article ID HEAD BLIGHT RESISTANCE; GENOMEWIDE SELECTION; QUANTITATIVE TRAITS; WIDE SELECTION; TRANSGRESSIVE VARIATION; ASSISTED SELECTION; MOLECULAR MARKERS; COMPLEX TRAITS; LOWLAND RICE; PREDICTION AB Genomic Selection (GS) is a new breeding method in which genome-wide markers are used to predict the breeding value of individuals in a breeding population. GS has been shown to improve breeding efficiency in dairy cattle and several crop plant species, and here we evaluate for the first time its efficacy for breeding inbred lines of rice. We performed a genome-wide association study (GWAS) in conjunction with five-fold GS cross-validation on a population of 363 elite breeding lines from the International Rice Research Institute's (IRRI) irrigated rice breeding program and herein report the GS results. The population was genotyped with 73,147 markers using genotyping-by-sequencing. The training population, statistical method used to build the GS model, number of markers, and trait were varied to determine their effect on prediction accuracy. For all three traits, genomic prediction models outperformed prediction based on pedigree records alone. Prediction accuracies ranged from 0.31 and 0.34 for grain yield and plant height to 0.63 for flowering time. Analyses using subsets of the full marker set suggest that using one marker every 0.2 cM is sufficient for genomic selection in this collection of rice breeding materials. RR-BLUP was the best performing statistical method for grain yield where no large effect QTL were detected by GWAS, while for flowering time, where a single very large effect QTL was detected, the non-GS multiple linear regression method outperformed GS models. For plant height, in which four mid-sized QTL were identified by GWAS, random forest produced the most consistently accurate GS models. Our results suggest that GS, informed by GWAS interpretations of genetic architecture and population structure, could become an effective tool for increasing the efficiency of rice breeding as the costs of genotyping continue to decline. C1 [Spindel, Jennifer; Akdemir, Deniz; Jannink, Jean-Luc; McCouch, Susan R.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Begum, Hasina; Collard, Bertrand; Redona, Edilberto] Int Rice Res Inst, Los Banos, Philippines. [Virk, Parminder] Ctr Int Agr Trop, Cali, Colombia. [Atlin, Gary] Bill & Melinda Gates Fdn, Seattle, WA USA. [Jannink, Jean-Luc] ARS, USDA, Ithaca, NY USA. RP Spindel, J (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. EM jj332@cornell.edu; srm4@cornell.edu RI Spindel, Jennifer/F-1373-2017 FU National Science Foundation Plant Genome Research Project [102655]; National Science Foundation Graduate Research Fellowship FX Funding for this project was provided by the National Science Foundation Plant Genome Research Project grant #102655 www.nsf.gov, a National Science Foundation Graduate Research Fellowship to JS, the Organization for Women in Science for the Developing World, and the Japan Rice Breeding Project at the International Rice Research Institute. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 68 TC 54 Z9 56 U1 9 U2 62 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7390 EI 1553-7404 J9 PLOS GENET JI PLoS Genet. PD FEB PY 2015 VL 11 IS 2 AR e1004982 DI 10.1371/journal.pgen.1004982 PG 25 WC Genetics & Heredity SC Genetics & Heredity GA CE8HF UT WOS:000352081800038 PM 25689273 ER PT J AU Wang, GF Ji, JB Ei-Kasmi, F Dangl, JL Johal, G Balint-Kurti, PJ AF Wang, Guan-Feng Ji, Jiabing Ei-Kasmi, Farid Dangl, Jeffery L. Johal, Guri Balint-Kurti, Peter J. TI Molecular and Functional Analyses of a Maize Autoactive NB-LRR Protein Identify Precise Structural Requirements for Activity SO PLOS PATHOGENS LA English DT Article ID PLANT-DISEASE RESISTANCE; ASSISTED GENE IDENTIFICATION; RICH REPEAT DOMAINS; POTATO-VIRUS-X; CELL-DEATH; HYPERSENSITIVE RESPONSE; COILED-COIL; PHYSICAL ASSOCIATION; TRANSIENT EXPRESSION; ARTIFICIAL EVOLUTION AB Plant disease resistance is often mediated by nucleotide binding-leucine rich repeat (NLR) proteins which remain auto-inhibited until recognition of specific pathogen-derived molecules causes their activation, triggering a rapid, localized cell death called a hypersensitive response (HR). Three domains are recognized in one of the major classes of NLR proteins: a coiled-coil (CC), a nucleotide binding (NB-ARC) and a leucine rich repeat (LRR) domains. The maize NLR gene Rp1-D21 derives from an intergenic recombination event between two NLR genes, Rp1-D and Rp1-dp2 and confers an autoactive HR. We report systematic structural and functional analyses of Rp1 proteins in maize and N. benthamiana to characterize the molecular mechanism of NLR activation/auto-inhibition. We derive a model comprising the following three main features: Rp1 proteins appear to self-associate to become competent for activity. The CC domain is signaling-competent and is sufficient to induce HR. This can be suppressed by the NB-ARC domain through direct interaction. In autoactive proteins, the interaction of the LRR domain with the NB-ARC domain causes de-repression and thus disrupts the inhibition of HR. Further, we identify specific amino acids and combinations thereof that are important for the auto-inhibition/activity of Rp1 proteins. We also provide evidence for the function of MHD2, a previously uncharacterized, though widely conserved NLR motif. This work reports several novel insights into the precise structural requirement for NLR function and informs efforts towards utilizing these proteins for engineering disease resistance. C1 [Wang, Guan-Feng; Balint-Kurti, Peter J.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Ji, Jiabing; Johal, Guri] Purdue Univ, Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Ei-Kasmi, Farid; Dangl, Jeffery L.] Univ N Carolina, Dept Biol, Chapel Hill, NC USA. [Dangl, Jeffery L.] Univ N Carolina, Dept Microbiol & Immunol, Howard Hughes Med Inst, Curriculum Genet & Mol Biol, Chapel Hill, NC USA. [Balint-Kurti, Peter J.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Wang, GF (reprint author), N Carolina State Univ, Dept Plant Pathol, Box 7616, Raleigh, NC 27695 USA. EM gwang11@ncsu.edu; peter.balint-kurti@ars.usda.gov OI Balint-Kurti, Peter/0000-0002-3916-194X FU National Science Foundation [0822495]; U.S. Department of Agriculture-Agricultural Research Service; German science foundation (DFG) [EL734/1-1]; NSF [IOS-1257373]; HHMI; Gordon and Betty Moore Foundation [GBMF3030] FX Funding: This work was supported by National Science Foundation (grant no. 0822495) and the U.S. Department of Agriculture-Agricultural Research Service. FEK is funded by a German science foundation (DFG) postdoctoral fellowship (EL734/1-1). Work in the lab of JLD was supported by NSF grant IOS-1257373 to JLD. JLD is an Investigator of the Howard Hughes Medical Institute and is supported by the HHMI and the Gordon and Betty Moore Foundation (GBMF3030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 15 Z9 15 U1 5 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD FEB PY 2015 VL 11 IS 2 AR UNSP e1004674 DI 10.1371/journal.ppat.1004674 PG 32 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA CE8HU UT WOS:000352083400050 PM 25719542 ER PT J AU De Graff, JV Shelmerdine, B Gallegos, A Annis, D AF De Graff, Jerome V. Shelmerdine, Bill Gallegos, Alan Annis, David TI Uncertainty Associated with Evaluating Rockfall Hazard to Roads in Burned Areas SO ENVIRONMENTAL & ENGINEERING GEOSCIENCE LA English DT Article DE Rockfall; Wildfires; Roads; Western USA; Natural Hazards ID GEOMORPHIC RESPONSE; SEDIMENT TRANSPORT; UNITED-STATES; DEBRIS FLOWS; WILDFIRE; CALIFORNIA; STEEP; FIRE; LANDSCAPES AB During and following wildfires affecting steep mountain slopes, there can be an increase in rockfall activity usually taking the form of individual rocks, and occasionally, groups of rocks rolling, sliding or bouncing downslope. This increase results from removal of stabilizing vegetation, downed wood, and organics within the soil matrix as well as increase in erosional processes such as dry ravel. The hazard posed to vehicles is difficult to assess because of uncertainty manifested in several ways. First, there is uncertainty in defining the road segments that will be impacted by increased rockfall activity. Second, it is difficult to quantify the size, number, and/or travel behavior of rocks which may impact a given road segment. Finally, there is uncertainty as to how long increased rockfall activity may persist after a wildfire. Between 2007 and 2013, some insight into the first two uncertainty issues was provided by observed rockfall on roads within eight different wildfires in California and Idaho. This insight provided an efficient and effective means to prioritize rapid assessment for rockfall hazard for a large number of roads within the 2013 Rim Fire in the central Sierra Nevada, California. Data on the third rockfall uncertainty issue, persistence, was developed for a road on the Olympic National Forest in Washington. Monitoring of rocks accumulating on the road at sixteen sites between July 2006 and April 2007 recorded 3,463 rocks with the number of rocks found to decrease over time. C1 [De Graff, Jerome V.; Gallegos, Alan] US Forest Serv, USDA, Clovis, CA 93611 USA. [Shelmerdine, Bill] Olymp Natl Forest, Olympia, WA 98512 USA. [Annis, David] Eldorado Natl Forest, Placerville, CA 95667 USA. RP De Graff, JV (reprint author), US Forest Serv, USDA, 1600 Tollhouse Rd, Clovis, CA 93611 USA. EM 45nyutca@sbcglobal.net NR 39 TC 0 Z9 0 U1 2 U2 10 PU GEOLOGICAL SOC AMER, INC PI BOULDER PA PO BOX 9140, BOULDER, CO 80301-9140 USA SN 1078-7275 EI 1558-9161 J9 ENVIRON ENG GEOSCI JI Environ. Eng. Geosci. PD FEB PY 2015 VL 21 IS 1 BP 21 EP 33 PG 13 WC Engineering, Environmental; Engineering, Geological; Geosciences, Multidisciplinary SC Engineering; Geology GA CE8LW UT WOS:000352094900002 ER PT J AU Harris, MO Friesen, TL Xu, SS Chen, MS Giron, D Stuart, JJ AF Harris, M. O. Friesen, T. L. Xu, S. S. Chen, M. S. Giron, D. Stuart, J. J. TI Pivoting from Arabidopsis to wheat to understand how agricultural plants integrate responses to biotic stress SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Review DE Avirulence gene; effector-triggered susceptibility; effector-triggered immunity; gene-for-gene interactions; Resistance gene; Susceptibility gene; Triticeae ID HESSIAN FLY DIPTERA; GENE-FOR-GENE; POWDERY MILDEW RESISTANCE; TRITICUM-AESTIVUM L.; MAP-BASED CLONING; DISEASE-SUSCEPTIBILITY GENE; MAYETIOLA-DESTRUCTOR LARVAE; SOUTHEASTERN UNITED-STATES; MULTIPLE FUNGAL PATHOGENS; SALIVARY-GLAND PROTEINS AB In this review, we argue for a research initiative on wheat's responses to biotic stress. One goal is to begin a conversation between the disparate communities of plant pathology and entomology. Another is to understand how responses to a variety of agents of biotic stress are integrated in an important crop. We propose gene-for-gene interactions as the focus of the research initiative. On the parasite's side is an Avirulence (Avr) gene that encodes one of the many effector proteins the parasite applies to the plant to assist with colonization. On the plant's side is a Resistance (R) gene that mediates a surveillance system that detects the Avr protein directly or indirectly and triggers effector-triggered plant immunity. Even though arthropods are responsible for a significant proportion of plant biotic stress, they have not been integrated into important models of plant immunity that come from plant pathology. A roadblock has been the absence of molecular evidence for arthropod Avr effectors. Thirty years after this evidence was discovered in a plant pathogen, there is now evidence for arthropods with the cloning of the Hessian fly's vH13 Avr gene. After reviewing the two models of plant immunity, we discuss how arthropods could be incorporated. We end by showing features that make wheat an interesting system for plant immunity, including 479 resistance genes known from agriculture that target viruses, bacteria, fungi, nematodes, insects, and mites. It is not likely that humans will be subsisting on Arabidopsis in the year 2050. It is time to start understanding how agricultural plants integrate responses to biotic stress. C1 [Harris, M. O.] N Dakota State Univ, Dept Entomol, Fargo, ND 58105 USA. [Friesen, T. L.; Xu, S. S.] USDA ARS, Cereal Crops Res Unit, Fargo, ND USA. [Chen, M. S.] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Giron, D.] Univ Tours, Inst Rech Biol Insecte, CNRS, UMR 7261, Tours, France. [Stuart, J. J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. RP Harris, MO (reprint author), N Dakota State Univ, Dept Entomol, Fargo, ND 58105 USA. EM Marion.Harris@ndsu.edu RI Giron, David/B-9108-2013 OI Giron, David/0000-0001-8356-0983 FU United States Department of Agriculture, National Institute of Food and Agriculture (NIFA) [2011-67013-30211] FX We thank Kirk Anderson for comments on an earlier draft and Jason Axtman for help compiling references for tables. We thank the United States Department of Agriculture, National Institute of Food and Agriculture (NIFA), for funding decades of research on the Hessian fly, most recently: 'Insect Effectors in Molecular Plant-Insect Interactions', grant no. 2011-67013-30211. MOH and DG also thank Le Studium (R), Loire Valley, Institute for Advanced Studies. Many of the ideas in this review were developed during a Le Studium (R) fellowship. NR 176 TC 14 Z9 14 U1 10 U2 43 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD FEB PY 2015 VL 66 IS 2 SI SI BP 513 EP 531 DI 10.1093/jxb/eru465 PG 19 WC Plant Sciences SC Plant Sciences GA CE2QW UT WOS:000351661400009 PM 25504642 ER PT J AU Pallipparambil, GR Sayler, RJ Shapiro, JP Thomas, JMG Kring, TJ Goggin, FL AF Pallipparambil, Godshen R. Sayler, Ronald J. Shapiro, Jeffrey P. Thomas, Jean M. G. Kring, Timothy J. Goggin, Fiona L. TI Mi-1.2, an R gene for aphid resistance in tomato, has direct negative effects on a zoophytophagous biocontrol agent, Orius insidiosus SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Biological control; host plant resistance; insect resistance; integrated pest management; Mi-1; Mi-1.2; mesophyll feeding; natural enemy; plant defence; R gene; xylem feeding ID COCCINELLA-SEPTEMPUNCTATA COLEOPTERA; RUSSIAN WHEAT APHID; POTATO APHID; PLANT-RESISTANCE; MACROSIPHUM-EUPHORBIA; MEDIATED RESISTANCE; BINODOXYS-COMMUNIS; BEMISIA-TABACI; MI; NEMATODE AB Mi-1.2 is a single dominant gene in tomato that confers race-specific resistance against certain phloem-feeding herbivores including aphids, whiteflies, psyllids, and root-knot nematodes. Few prior studies have considered the potential non-target effects of race-specific resistance genes (R genes), and this paper evaluates the compatibility of Mi-mediated resistance in tomato with a beneficial zoophytophagous predator, Orius insidiosus (Say). In addition to preying on aphids and other pests, this piercing-sucking insect also feeds from the xylem, epidermis, and/or mesophyll, and oviposits within plant tissues. Comparison of O. insidiosus confined to isogenic tomato plants with and without Mi-1.2 revealed that immatures of O. insidiosus had lower survival on resistant plants even when the immatures were provisioned with prey that did not feed on the host plant. Molecular gut content analysis confirmed that adults and immatures of O. insidiosus feed on both resistant (Mi-1.2+) and susceptible (Mi-1.2-) genotypes, and bio-assays suggest that resistance does not affect oviposition rates, plant sampling, or prey acceptance by O. insidiosus adults. These results demonstrate a direct negative impact of R-gene-mediated host plant resistance on a non-target beneficial species, and reveal that Mi-mediated resistance can impact organisms that do not feed on phloem sap. Through laser capture microdissection and RT-PCR, Mi-1.2 transcripts were detected in the epidermis and mesophyll as well as the phloem of tomato plants, consistent with our observations that Mi-mediated resistance is active outside the phloem. These results suggest that the mode of action and potential ecological impacts of Mi-mediated resistance are broader than previously assumed. C1 [Pallipparambil, Godshen R.; Kring, Timothy J.; Goggin, Fiona L.] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. [Sayler, Ronald J.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Shapiro, Jeffrey P.; Thomas, Jean M. G.] ARS, USDA, CMAVE, Gainesville, FL 32608 USA. RP Goggin, FL (reprint author), Univ Arkansas, Dept Entomol, 319 Agr Bldg, Fayetteville, AR 72701 USA. EM fgoggin@uark.edu FU National Research Initiative of the USDA Cooperative State Research, Education and Extension Service Grant [2006-35607-330 16612] FX We thank Clinton Trammel, Lingling Jia, Jun Shen, Carlos Avila, Jingjing Xie, Fengying Shi, Bob Dienglewicz, David McNabb, Andronikos Mauromoustakos, John Guerber, Allen Szalanski, Ashley Dowling, Ken Korth, Wayne Kuenzel, Ines Pinto, Hong Li Wang, and Gisela Erf for advice and assistance with experiments, equipment, and statistics. This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service Grant 2006-35607-330 16612 awarded to FLG. NR 48 TC 2 Z9 3 U1 4 U2 33 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD FEB PY 2015 VL 66 IS 2 SI SI BP 549 EP 557 DI 10.1093/jxb/eru361 PG 9 WC Plant Sciences SC Plant Sciences GA CE2QW UT WOS:000351661400012 PM 25189594 ER PT J AU Yan, J Lipka, AE Schmelz, EA Buckler, ES Jander, G AF Yan, Jian Lipka, Alexander E. Schmelz, Eric A. Buckler, Edward S. Jander, Georg TI Accumulation of 5-hydroxynorvaline in maize (Zea mays) leaves is induced by insect feeding and abiotic stress SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Aphid; drought; 5-hydroxynorvaline; maize; Rhopalosiphum maidis ID ASSOCIATION MAPPING POPULATION; DROUGHT STRESS; AMINO-ACIDS; SHORT-TERM; DEFENSE; BIOSYNTHESIS; RESISTANT; GENETICS; ATTACK; LEPIDOPTERA AB Plants produce a wide variety of defensive metabolites to protect themselves against herbivores and pathogens. Nonprotein amino acids, which are present in many plant species, can have a defensive function through their mis-incorporation during protein synthesis and/or inhibition of biosynthetic pathways in primary metabolism. 5-Hydroxynorvaline was identified in a targeted search for previously unknown non-protein amino acids in the leaves of maize (Zea mays) inbred line B73. Accumulation of this compound increases during herbivory by aphids (Rhopalosiphum maidis, corn leaf aphid) and caterpillars (Spodoptera exigua, beet armyworm), as well as in response to treatment with the plant signalling molecules methyl jasmonate, salicylic acid and abscisic acid. In contrast, ethylene signalling reduced 5-hydroxynorvaline abundance. Drought stress induced 5-hydroxynorvaline accumulation to a higher level than insect feeding or treatment with defence signalling molecules. In field-grown plants, the 5-hydroxynorvaline concentration was highest in above-ground vegetative tissue, but it was also detectable in roots and dry seeds. When 5-hydroxynorvaline was added to aphid artificial diet at concentrations similar to those found in maize leaves and stems, R. maidis reproduction was reduced, indicating that this maize metabolite may have a defensive function. Among 27 tested maize inbred lines there was a greater than 10-fold range in the accumulation of foliar 5-hydroxynorvaline. Genetic mapping populations derived from a subset of these inbred lines were used to map quantitative trait loci for 5-hydroxynorvaline accumulation to maize chromosomes 5 and 7. C1 [Yan, Jian; Jander, Georg] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Lipka, Alexander E.; Buckler, Edward S.] Cornell Univ, Inst Genom Divers, Ithaca, NY USA. [Schmelz, Eric A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Chem Res Unit, Gainesville, FL USA. [Buckler, Edward S.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. [Buckler, Edward S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY USA. RP Jander, G (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM gj32@cornell.edu OI Schmelz, Eric/0000-0002-2837-734X; Buckler, Edward/0000-0002-3100-371X FU NSF [IOS-1139329, IOS-0922493]; Science & Technology Star of Zhujiang, Guangzhou City [2013J2200082] FX This research was funded by NSF awards IOS-1139329 to GJ and EAS, and IOS-0922493 to ESB, and a fellowship from Science & Technology Star of Zhujiang, Guangzhou City (2013J2200082) to JY. NR 43 TC 5 Z9 5 U1 3 U2 28 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD FEB PY 2015 VL 66 IS 2 SI SI BP 593 EP 602 DI 10.1093/jxb/eru385 PG 10 WC Plant Sciences SC Plant Sciences GA CE2QW UT WOS:000351661400016 PM 25271262 ER PT J AU Lutter, R Abbott, L Becker, R Borgert, C Bradley, A Charnley, G Dudley, S Felsot, A Golden, N Gray, G Juberg, D Mitchell, M Rachman, N Rhomberg, L Solomon, K Sundlof, S Willett, K AF Lutter, Randall Abbott, Linda Becker, Rick Borgert, Chris Bradley, Ann Charnley, Gail Dudley, Susan Felsot, Alan Golden, Nancy Gray, George Juberg, Daland Mitchell, Mary Rachman, Nancy Rhomberg, Lorenz Solomon, Keith Sundlof, Stephen Willett, Kate TI Improving Weight of Evidence Approaches to Chemical Evaluations SO RISK ANALYSIS LA English DT Article DE Chemical evaluation; hypothesis; weight of evidence ID HYPOTHESIS-BASED WEIGHT; IPCS FRAMEWORK; MODE; RELEVANCE; HUMANS AB Federal and other regulatory agencies often use or claim to use a weight of evidence (WoE) approach in chemical evaluation. Their approaches to the use of WoE, however, differ significantly, rely heavily on subjective professional judgment, and merit improvement. We review uses of WoE approaches in key articles in the peer-reviewed scientific literature, and find significant variations. We find that a hypothesis-based WoE approach, developed by Lorenz Rhomberg et al., can provide a stronger scientific basis for chemical assessment while improving transparency and preserving the appropriate scope of professional judgment. Their approach, while still evolving, relies on the explicit specification of the hypothesized basis for using the information at hand to infer the ability of an agent to cause human health impacts or, more broadly, affect other endpoints of concern. We describe and endorse such a hypothesis-based WoE approach to chemical evaluation. C1 [Lutter, Randall] Univ Virginia, Batten Sch Leadership & Publ Policy, Charlottesville, VA 22904 USA. [Abbott, Linda] USDA, Off Chief Economist, Washington, DC USA. [Becker, Rick] Amer Chem Council, Washington, DC USA. [Borgert, Chris] Appl Pharmacol & Toxicol Inc, Washington, DC USA. [Bradley, Ann] Integral Consulting Inc, Seattle, WA USA. [Charnley, Gail] Hlth Risk Strategies, Washington, DC USA. [Dudley, Susan; Gray, George] George Washington Univ, Washington, DC 20052 USA. [Felsot, Alan] Washington State Univ, Pullman, WA 99164 USA. [Golden, Nancy] US Fish & Wildlife Serv, Washington, DC USA. [Juberg, Daland] Dow AgroSci, Indianapolis, IN USA. [Mitchell, Mary] Pest Management Regulatory Agcy Canada, Ottawa, ON, Canada. [Rachman, Nancy] ILSI North Amer, Washington, DC USA. [Rhomberg, Lorenz] Gradient Corp, Cambridge, MA USA. [Solomon, Keith] Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada. [Sundlof, Stephen] Univ Maryland, Coll Agr & Nat Resources, Baltimore, MD USA. [Willett, Kate] Humane Soc United States, Washington, DC USA. RP Lutter, R (reprint author), Univ Virginia, Frank Batten Sch Leadership & Publ Policy, B007 Garrett Hall,235 McCormick Rd, Charlottesville, VA 22904 USA. EM randall.lutter@virginia.edu FU CropLife America FX Dr. Lutter received financial support from CropLife America to moderate a forum and serve as principal author. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of their employers or other organizations with which they may be associated, including the USDA, U.S. Fish and Wildlife Service, or the Pest Management Regulatory Agency of Canada. NR 28 TC 6 Z9 7 U1 4 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD FEB PY 2015 VL 35 IS 2 BP 186 EP 192 DI 10.1111/risa.12277 PG 7 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA CE2XD UT WOS:000351684000003 PM 25516407 ER PT J AU Haas, JR Calkin, DE Thompson, MP AF Haas, Jessica R. Calkin, David E. Thompson, Matthew P. TI Wildfire Risk Transmission in the Colorado Front Range, USA SO RISK ANALYSIS LA English DT Article DE Exposure analysis; human populated areas; Randig; risk transmission; wildfires ID WILDLAND-URBAN INTERFACE; UNITED-STATES; FIRE REGIMES; EXPOSURE; FOREST; SIMULATION; MANAGEMENT; CLIMATE; SPREAD; MODEL AB Wildfires are a global phenomenon that in some circumstances can result in human casualties, economic loss, and ecosystem service degradation. In this article we spatially identify wildfire risk transmission pathways and locate the areas of highest exposure of human populations to wildland fires under severe, but not uncommon, weather events. We quantify varying levels of exposure in terms of population potentially affected and tie the exposure back to the spatial source of the risk for the Front Range of Colorado, USA. We use probabilistic fire simulation modeling to address where fire ignitions are most likely to cause the highest impact to human communities, and to explore the role that various landowners play in that transmission of risk. Our results indicated that, given an ignition and the right fire weather conditions, large areas along the Front Range in Colorado could be exposed to wildfires with high potential to impact human populations, and that overall private ignitions have the potential to impact more people than federal ignitions. These results can be used to identify high-priority areas for wildfire risk mitigation using various mitigation tools. C1 [Haas, Jessica R.; Calkin, David E.; Thompson, Matthew P.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59802 USA. RP Haas, JR (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 200 E Broadway, Missoula, MT 59802 USA. EM jrhaas@fs.fed.us NR 52 TC 13 Z9 13 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD FEB PY 2015 VL 35 IS 2 BP 226 EP 240 DI 10.1111/risa.12270 PG 15 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA CE2XD UT WOS:000351684000006 PM 25156542 ER PT J AU Wang, WX Mozuch, MD Sabo, RC Kersten, P Zhu, JY Jin, YC AF Wang, Wangxia Mozuch, Michael D. Sabo, Ronald C. Kersten, Phil Zhu, J. Y. Jin, Yongcan TI Production of cellulose nanofibrils from bleached eucalyptus fibers by hyperthermostable endoglucanase treatment and subsequent microfluidization SO CELLULOSE LA English DT Article DE Hyperthermophilic endoglucanase; Pretreatment; Microfluidization; Nanofibrillated cellulose ID PYROCOCCUS-HORIKOSHII; HYPERTHERMOPHILIC CELLULASE; HIGH TOUGHNESS; NANOCOMPOSITES; TRANSPARENT; FIBRILS AB A GH5 hyperthermostable endoglucanase from the archaeon Pyrococcus horikoshii (Ph-GH5) and a commercial endoglucanase FR were used to treat bleached eucalyptus pulp (BEP) fibers to produce cellulose nanofibrils (CNFs) through subsequent microfluidization. Enzymatic treatments facilitated CNF production due to the reduced degree of polymerization (DP) of the fibers. SEM imaging indicated that FR reduced fiber DP drastically and resulted in much shorter fibers than with Ph-GH5, even at very low dosages (1 mg protein/g fiber) of FR treatment compared with a high dosage (10 mg protein/g fiber) of Ph-GH5. The fibers treated with FR were much more uniform in length perhaps due to the presence of exoglucanase and beta-glucosidase saccharifying short microfibers into glucose. TEM imaging indicated that Ph-GH5 produced longer and entangled CNFs than FR with the same number of microfluidization passes. However, the CNF diameters were approximately the same for all CNFs from enzyme-treated fibers using both endoglucanases at two dosages (1 or 10 mg protein/g fiber). CNFs produced from BEP fibers without enzymatic treatment showed larger diameters than those with enzymatic treatment. C1 [Wang, Wangxia; Jin, Yongcan] Nanjing Forestry Univ, Jiangsu Prov Key Lab Pulp & Paper Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China. [Wang, Wangxia; Mozuch, Michael D.; Sabo, Ronald C.; Kersten, Phil; Zhu, J. Y.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Kersten, P (reprint author), US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM pkersten@fs.fed.us; jzhu@fs.fed.us FU USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant [2011-67009-20056]; Chinese Scholarship Council (CSC); National Natural Science Foundation of China [31070512, 31370571] FX We acknowledge the financial supports by a USDA Agriculture and Food Research Initiative (AFRI) Competitive Grant (No. 2011-67009-20056), Chinese Scholarship Council (CSC), National Natural Science Foundation of China (Grant Nos. 31070512 and 31370571). The funding from these programs made the visiting appointment of Wang at the USDA Forest Products Laboratory (FPL) possible. We also acknowledge Thomas Kuster of the Analytical Chemistry and Microscopy Lab of FPL for SEM imaging and Debra Sherman of DS imaging LLC, West Lafayette, IN, for TEM imaging. NR 30 TC 13 Z9 13 U1 3 U2 33 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD FEB PY 2015 VL 22 IS 1 BP 351 EP 361 DI 10.1007/s10570-014-0465-2 PG 11 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA CD2AY UT WOS:000350877100029 ER PT J AU Wada, S Niedz, RP Reed, BM AF Wada, Sugae Niedz, Randall P. Reed, Barbara M. TI Determining nitrate and ammonium requirements for optimal in vitro response of diverse pear species SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Growth medium; Micropropagation; Mineral nutrition; Nitrogen; Pyrus; Response surface design ID SUSPENSION-CULTURES; NITROGEN; GROWTH; NUTRITION; TOBACCO; TISSUE; INVITRO; LEAVES; PLANTS AB Inorganic nitrate (NO3 (-)) and ammonium (NH4 (+)) are the two major components in nitrogen (N) nutrition of typical tissue culture growth media, and the total amounts and ratios influence both shoot induction and differentiation. This study was designed to determine the optimal N requirements and interactions of NH4 (+) x NO3 (-) to complete the optimization of a pear shoot culture medium. Pyrus communis 'Horner 51' and 'OH x F 87', P. cordata, P. pyrifolia 'Sion Szu Mi', and P. ussuriensis 'Hang Pa Li' from the pear germplasm collection of the US Department of Agriculture, National Clonal Germplasm Repository-Corvallis (NCGR) were evaluated. Response surface design was used to create and analyze treatment combinations of NH4 (+), K+, and NO3 (-). Cultures were evaluated for overall quality, shoot length, multiplication, leaf color and size, leaf spotting and necrosis, and callus production. Significant improvement was observed in multiplication and length for most genotypes. Reduced callus amounts were seen in two genotypes, and greener leaves were also seen in two genotypes. Each species had a distinct response, and the N form could be manipulated to produce longer shoots, more shoots, or less callus. For the best-quality shoots, both P. communis cultivars required high NO3 (-) and low to moderate NH4 (+), P. cordata quality was best with high NO3 (-) and NH4 (+), P. pyrifolia 'Sion Szu Mi' quality improved with moderate NO3 (-) and high NH4 (+), and P. ussuriensis 'Hang Pa Li' required low NO3 (-) and high NH4 (+). This study illustrates that optimizing the N components of a growth medium is very important when working with diverse plant germplasm. C1 [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Niedz, Randall P.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Reed, Barbara M.] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Wada, S (reprint author), Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. EM Sugae.Wada@Oregonstate.edu; Randall.Niedz@ars.usda.gov; Barbara.Reed@ars.usda.gov FU Oregon Association of Nurseries; Oregon Department of Agriculture; Department of Agriculture, Agricultural Research Service CRIS [5358-21000-038-00D] FX We thank the NCGR lab personnel for the assistance with data collection. This project was funded by a grant from the Oregon Association of Nurseries and the Oregon Department of Agriculture and by US Department of Agriculture, Agricultural Research Service CRIS project 5358-21000-038-00D. NR 34 TC 5 Z9 5 U1 1 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD FEB PY 2015 VL 51 IS 1 BP 19 EP 27 DI 10.1007/s11627-015-9662-4 PG 9 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA CD5DD UT WOS:000351107200003 ER PT J AU Chen, M Griffis, TJ Baker, J Wood, JD Xiao, K AF Chen, Ming Griffis, Tim J. Baker, John Wood, Jeffrey D. Xiao, Ke TI Simulating crop phenology in the Community Land Model and its impact on energy and carbon fluxes SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE crop modeling; Community Land Model; crop phenology; net ecosystem CO2 exchange; soil water content ID CLIMATE SYSTEM MODEL; INTERANNUAL VARIABILITY; PHOTOSYNTHETIC CAPACITY; RICHARDS EQUATION; ECOSYSTEM MODEL; USE EFFICIENCY; WATER; NITROGEN; CORN; BALANCE AB A reasonable representation of crop phenology and biophysical processes in land surface models is necessary to accurately simulate energy, water, and carbon budgets at the field, regional, and global scales. However, the evaluation of crop models that can be coupled to Earth system models is relatively rare. Here we evaluated two such models (CLM4-Crop and CLM3.5-CornSoy), both implemented within the Community Land Model (CLM) framework, at two AmeriFlux corn-soybean sites to assess their ability to simulate phenology, energy, and carbon fluxes. Our results indicated that the accuracy of net ecosystem exchange and gross primary production simulations was intimately connected to the phenology simulations. The CLM4-Crop model consistently overestimated early growing season leaf area index, causing an overestimation of gross primary production, to such an extent that the model simulated a carbon sink instead of the measured carbon source for corn. The CLM3.5-CornSoy-simulated leaf area index (LAI), energy, and carbon fluxes showed stronger correlations with observations compared to CLM4-Crop. Net radiation was biased high in both models and was especially pronounced for soybeans. This was primarily caused by the positive LAI bias, which led to a positive net long-wave radiation bias. CLM4-Crop underestimated soil water content during midgrowing season in all soil layers at the two sites, which caused unrealistic water stress, especially for soybean. Future work regarding the mechanisms that drive early growing season phenology and soil water dynamics is needed to better represent crops including their net radiation balance, energy partitioning, and carbon cycle processes. C1 [Chen, Ming; Griffis, Tim J.; Baker, John; Wood, Jeffrey D.; Xiao, Ke] Univ Minnesota Twin Cities, Dept Soil Water & Climate, St Paul, MN 55102 USA. [Baker, John] USDA ARS, St Paul, MN USA. RP Chen, M (reprint author), Univ Minnesota Twin Cities, Dept Soil Water & Climate, St Paul, MN 55102 USA. EM chen1718@umn.edu RI Griffis, Timothy/A-5707-2011; OI Chen, Ming/0000-0002-4574-632X FU National Science Foundation [ATM-0546476]; United States Department of Agriculture [USDA-NIFA 2013-67019-21364]; U.S. Department of Energy's Office of Science; University of Minnesota Supercomputing Institute FX We express our sincere thanks to Jeremy Smith and Bill Breiter for their technical assistance in the laboratory and at the field site. Funding for this research has been provided by the National Science Foundation (ATM-0546476) and the United States Department of Agriculture (USDA-NIFA 2013-67019-21364). Funding for this AmeriFlux core site was provided by the U.S. Department of Energy's Office of Science. We are grateful to the University of Minnesota Supercomputing Institute for partial support of this work. We would like to thank Sam Levis at the National Center for Atmospheric Research for his helpful discussions and support regarding the CLM4-Crop model. We would also like to thank Mark Lostetter and Patricia Backes at the Rosemount Research and Outreach Center who provided us the yield data at the two sites. All of the meteorological and flux data used in this study are freely available at the biometeorology group UMN website (http://www.biometeorology.umn.edu/data_archive.php). NR 78 TC 7 Z9 7 U1 5 U2 31 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD FEB PY 2015 VL 120 IS 2 BP 310 EP 325 DI 10.1002/2014JG002780 PG 16 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CD9FI UT WOS:000351402800009 ER PT J AU Hwang, T Band, LE Hales, TC Miniat, CF Vose, JM Bolstad, PV Miles, B Price, K AF Hwang, Taehee Band, Lawrence E. Hales, T. C. Miniat, Chelcy F. Vose, James M. Bolstad, Paul V. Miles, Brian Price, Katie TI Simulating vegetation controls on hurricane-induced shallow landslides with a distributed ecohydrological model SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE landslide modeling; root cohesion; lidar; slope stability; belowground biomass ID RHODODENDRON-MAXIMUM L; FOREST INVENTORY DATA; LANDSAT TM DATA; ABOVEGROUND BIOMASS; ROOT-REINFORCEMENT; SATELLITE DATA; ETM+ DATA; LIDAR; ALLOCATION; PHOTOSYNTHESIS AB The spatial distribution of shallow landslides in steep forested mountains is strongly controlled by aboveground and belowground biomass, including the distribution of root cohesion. While remote sensing of aboveground canopy properties is relatively advanced, estimating the spatial distribution of root cohesion at the forest landscape scale remains challenging. We utilize canopy height information estimated using lidar (light detecting and ranging) technology as a tool to produce a spatially distributed root cohesion model for landslide hazard prediction. We characterize spatial patterns of total belowground biomass based on the empirically derived allometric relationship developed from soil pit measurements in the Coweeta Hydrologic Laboratory, North Carolina. The vertical distribution of roots and tensile strength were sampled at soil pits allowing us to directly relate canopy height to root cohesion and use this model within a distributed ecohydrological modeling framework, providing transient estimates of runoff, subsurface flow, soil moisture, and pore pressures. We tested our model in mountainous southern Appalachian catchments that experienced a number of landslides during the 2004 hurricane season. Slope stability estimates under the assumption of spatially uniform root cohesion significantly underpredicted both the total number of landslides and the number of false positives, unfailed areas of the landscape that were predicted to fail. When we incorporate spatially distributed root cohesion, the accuracy of the slope stability forecast improves dramatically. With the growing availability of lidar data that can be used to infer belowground information, these methods may provide a wider utility for improving landslide hazard prediction and forecasting. C1 [Hwang, Taehee; Band, Lawrence E.] Univ N Carolina, Inst Environm, Chapel Hill, NC USA. [Band, Lawrence E.] Univ N Carolina, Dept Geog, Chapel Hill, NC USA. [Hales, T. C.] Cardiff Univ, Sch Earth & Ocean Sci, Cardiff CF10 3AX, S Glam, Wales. [Miniat, Chelcy F.] USDA Forest Serv, Coweeta Hydrol Lab, Otto, NC USA. [Vose, James M.] USDA Forest Serv, Southern Res Stn, Raleigh, NC USA. [Bolstad, Paul V.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Price, Katie] Georgia State Univ, Dept Geosci, Atlanta, GA 30303 USA. RP Hwang, T (reprint author), Indiana Univ, Dept Geog, Bloomington, IN 47405 USA. EM taehee@indiana.edu OI Miniat, Chelcy/0000-0002-3266-9783; Hales, Tristram/0000-0002-3330-3302 FU USDA Forest Service; Southern Research Station; National Science Foundation from the Long Term Ecological Research Program [DEB-0823293] FX We thank two anonymous reviewers who provided helpful comments on the manuscript. We also thank Dean Urban of Duke University for use of his soil depth data set and Rick Wooten of the North Carolina Geological Survey for sharing data on mapped failure locations. All data and simulation results in this paper are available from the authors upon requests. Spatial (GIS and lidar) data and model source codes (RHESSys and RHESSysCalibrator) are available from URL links cited in the paper. This study was funded by the USDA Forest Service, Southern Research Station, and National Science Foundation grant DEB-0823293 from the Long Term Ecological Research Program to the Coweeta LTER Program at the University of Georgia. NR 76 TC 4 Z9 4 U1 3 U2 19 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD FEB PY 2015 VL 120 IS 2 BP 361 EP 378 DI 10.1002/2014JG002824 PG 18 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA CD9FI UT WOS:000351402800012 ER PT J AU Bellugi, D Milledge, DG Dietrich, WE McKean, JA Perron, JT Sudderth, EB Kazian, B AF Bellugi, Dino Milledge, David G. Dietrich, William E. McKean, Jim A. Perron, J. Taylor Sudderth, Erik B. Kazian, Brian TI A spectral clustering search algorithm for predicting shallow landslide size and location SO JOURNAL OF GEOPHYSICAL RESEARCH-EARTH SURFACE LA English DT Article DE shallow landslides; landslide size; hillslope geomorphology; spectral clustering; search algorithm; computational modeling ID SLOPE STABILITY ANALYSIS; PHYSICALLY-BASED MODEL; DEBRIS FLOWS; HYDROLOGIC RESPONSE; FOREST MANAGEMENT; SOIL PROPERTIES; NEW-ZEALAND; RAINFALL; OREGON; STEEP AB The potential hazard and geomorphic significance of shallow landslides depend on their location and size. Commonly applied one-dimensional stability models do not include lateral resistances and cannot predict landslide size. Multidimensional models must be applied to specific geometries, which are not known a priori, and testing all possible geometries is computationally prohibitive. We present an efficient deterministic search algorithm based on spectral graph theory and couple it with a multidimensional stability model to predict discrete landslides in applications at scales broader than a single hillslope using gridded spatial data. The algorithm is general, assuming only that instability results when driving forces acting on a cluster of cells exceed the resisting forces on its margins and that clusters behave as rigid blocks with a failure plane at the soil-bedrock interface. This algorithm recovers predefined clusters of unstable cells of varying shape and size on a synthetic landscape, predicts the size, location, and shape of an observed shallow landslide using field-measured physical parameters, and is robust to modest changes in input parameters. The search algorithm identifies patches of potential instability within large areas of stable landscape. Within these patches will be many different combinations of cells with a Factor of Safety less than one, suggesting that subtle variations in local conditions (e.g., pore pressure and root strength) may determine the ultimate form and exact location at a specific site. Nonetheless, the tests presented here suggest that the search algorithm enables the prediction of shallow landslide size as well as location across landscapes. C1 [Bellugi, Dino; Perron, J. Taylor] MIT, Dept Earth Atmospher & Planetary Sci, Cambridge, MA 02139 USA. [Milledge, David G.] Univ Durham, Dept Geog, Durham, England. [Dietrich, William E.] Univ Calif Berkeley, Dept Earth & Planetary Sci, Berkeley, CA 94720 USA. [McKean, Jim A.] US Forest Serv, USDA, Rocky Mt Res Stn, Boise, ID USA. [Sudderth, Erik B.] Brown Univ, Dept Comp Sci, Providence, RI 02912 USA. [Kazian, Brian] Palantir Technol, Palo Alto, CA USA. RP Bellugi, D (reprint author), MIT, Dept Earth Atmospher & Planetary Sci, Cambridge, MA 02139 USA. EM dinob@mit.edu OI Milledge, David/0000-0003-4077-4898 FU United States Forest Service [09-JV-11221634-233]; NASA [ROSES 09-IDS09-0049]; NSF [NSF-EAR-0828047]; NERC [NER/S/A/2004/12248] FX This research was financially supported by grants from the United States Forest Service (09-JV-11221634-233), NASA (ROSES 09-IDS09-0049), NSF (NSF-EAR-0828047), and NERC (NER/S/A/2004/12248). All data used to evaluate the model are derived from previously published sources. Thanks to Kevin Schmidt for providing root cohesion and soil depth data and to Dave Montgomery for providing landslide dimension data. All data used in this study have been previously published, please see citations in the text. NR 96 TC 7 Z9 7 U1 2 U2 23 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-9003 EI 2169-9011 J9 J GEOPHYS RES-EARTH JI J. Geophys. Res.-Earth Surf. PD FEB PY 2015 VL 120 IS 2 BP 300 EP 324 DI 10.1002/2014JF003137 PG 25 WC Geosciences, Multidisciplinary SC Geology GA CD9AB UT WOS:000351386900008 ER PT J AU Seo, EY Cho, S Moon, JS Gotoh, T Kim, HG Domier, LL Lim, S Kim, KH Moon, JK Hammond, J Lim, HS Song, KH AF Seo, Eun-Young Cho, Seunghee Moon, Jae Sun Gotoh, Takafumi Kim, Hong Gi Domier, Leslie L. Lim, Seungmo Kim, Kil Hyun Moon, Jung-Kyung Hammond, John Lim, Hyoun-Sub Song, Ki Hak TI A High Throughput Soybean Gene Identification System Developed using Soybean Yellow Common Mosaic Virus (SYCMV) SO JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY LA English DT Article DE Soybean yellow common mosaic virus; Soybean cDNA library; Virus induced gene silencing; Gateway cloning system; Reverse genetics ID NICOTIANA-BENTHAMIANA; EXPRESSION; PREDICTION; VECTOR; PLANTS AB Soybean yellow common mosaic virus (SYCMV) was recently reported from Korea, and a subsequent survey of soybean fields found that SYCMV, Soybean yellow mottle mosaic virus (SYMMV), and Soybean mosaic virus (SMV) infections were widespread. SYCMV has recently been developed into a Virus Inducing Gene Silencing (VIGS) vector for use as a reverse genetics tool for soybean, and here we report a modified SYCMV VIGS vector containing a new restriction enzyme site in the 3' non coding region into which we inserted the Gateway system. Ultrasonically generated c.300 bp random fragments of Glycine max cDNA were inserted into the SYCMV VIGS vector, and individual colonies containing G. max cDNA were inoculated to cultivar Williams 82. We monitored the phenotype of inoculated soybean, and selected obvious visible phenotypes caused by SYCMV induced gene silencing which could enable annotation of gene function of unknown gene fragments inserted into SYCMV. Here, we describe development of a high throughput SYCMV VIGS vector for gene function identification in soybean. C1 [Seo, Eun-Young; Cho, Seunghee; Kim, Hong Gi; Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Coll Agr & Life Sci, Taejon 305764, South Korea. [Moon, Jae Sun; Lim, Seungmo] Korea Res Inst Biosci & Biotechnol, Greenbio Res Ctr, Taejon 305333, South Korea. [Gotoh, Takafumi] Kyushu Univ, Fac Agr, Kuju Agr Res Ctr, Oita, Japan. [Domier, Leslie L.] ARS, USDA, Washington, DC USA. [Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Urbana, IL USA. [Lim, Seungmo] Univ Sci & Technol UST, Biosyst & Bioengn Program, Taejon 305350, South Korea. [Kim, Kil Hyun; Moon, Jung-Kyung] Rural Dev Adm, Natl Inst Crop Sci, Suwon 441857, South Korea. [Hammond, John] ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. [Song, Ki Hak] Chungnam Natl Univ, Sch Med, Dept Urol, Taejon 305764, South Korea. RP Lim, HS (reprint author), Chungnam Natl Univ, Dept Appl Biol, Coll Agr & Life Sci, Taejon 305764, South Korea. EM john.hammond@usda.ars.gov; hyounlim@cnu.ac.kr; urosong@cnu.ac.kr FU Rural Development Administration, Republic of Korea [PJ008063, PJ009033] FX This work was supported by a grant from the Next-Generation BioGreen 21 Program (The National Center for GM Crops No. PJ008063 and Plant Molecular Breeding Center No. PJ009033), Rural Development Administration, Republic of Korea. NR 21 TC 1 Z9 2 U1 0 U2 2 PU KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS PI FUKUOKA-SHI PA 6-10-1 HAKOZAKI, HIGASHI-KU, FUKUOKA-SHI, 812-0053, JAPAN SN 0023-6152 J9 J FAC AGR KYUSHU U JI J. Fac. Agric. Kyushu Univ. PD FEB PY 2015 VL 60 IS 1 BP 127 EP 138 PG 12 WC Agriculture, Multidisciplinary SC Agriculture GA CD8FD UT WOS:000351329700018 ER PT J AU Ko, NY Kim, HS Kim, JK Cho, S Seo, EY Kwon, HR Yu, YM Gotoh, T Hammond, J Youn, YN Lim, HS AF Ko, Na-Yeon Kim, Hyun-Seung Kim, Jung-Kyu Cho, Seunghee Seo, Eun-Young Kwon, Hye-Ri Yu, Yong Man Gotoh, Takafumi Hammond, John Youn, Young Nam Lim, Hyoun-Sub TI Developing an Alternanthera Mosaic Virus Vector for Efficient Cloning of Whitefly cDNA RNAi to Screen Gene Function SO JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY LA English DT Article DE Alternanthera mosaic virus; Gateway cloning system; Bemisia tabaci; RNAi; Nicotiana benthamiana ID CHLOROPLAST PHOSPHOGLYCERATE KINASE; NICOTIANA-BENTHAMIANA; DROSOPHILA EMBRYOS; BEMISIA-TABACI; PROTEIN; PLANTS; INTERFERENCE; SEQUENCE; PATHOGENICITY; DETERMINANTS AB Plant viral vectors have shown significant promse for studies of gene function, through either up regulation or down regulation of gene expression. However, there have remained issues of efficiency of generating constructs, and of subcellular localization of expression; both issues are addressed here. Alternanthera mosaic virus (AltMV; genus Potexvirus) is distinguished from the type member of the genus, Potato virus X by features of viral movement and variation within triple gene block protein 1 (TGB1). AltMV TGB1 variants TGB1L88 and TGB1P88 confer strong and weak silencing suppression, respectively, depending on the presence of L or P at residue 88. Because AltMV replication is associated with chloroplasts, we compared the relative efficiency of RNA interference (RNAi) vectors derived from AltMV and Tobacco rattle virus (TRV) to silence a chloroplast encoded gene. An AltMV RNAi vector expressing a fragment of the chloroplast 13 ATPase gene reduced beta-ATPase expression 1.5 times more than the TRV RNAi vector expressing the same fragment. In addition, we used AltMV (TGB1P88) to create a whitefly (Bemisia tabaci) RNAi vector. For this purpose, we first introduced the Gateway cloning cassette into the AltMV multiple cloning site, into which polymerase chain reaction (PCR) products from a whitefly cDNA library could be easily cloned. Second, a mixture of five different PCR fragments of about 250 bp were used to test cloning efficiency of the newly created AltMV-P-att vector. Third, random 250 bp fragments of Gateway cDNA libraries from B. tabaci and Nicotiana benthamiana were efficiently cloned into the Gateway modified AltMV-att vector, demonstrating for the first time a high throughput RNAi system based on AltMV. This strategy could be applied to other RNAi systems. C1 [Ko, Na-Yeon; Kim, Hyun-Seung; Kim, Jung-Kyu; Cho, Seunghee; Seo, Eun-Young; Kwon, Hye-Ri; Yu, Yong Man; Youn, Young Nam; Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Taejon, South Korea. [Gotoh, Takafumi] Kyushu Univ, Fac Agr, Kuju Agr Res Ctr, Oita, Japan. [Hammond, John] ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Lim, HS (reprint author), Chungnam Natl Univ, Dept Appl Biol, Taejon, South Korea. EM john.hammond@usda.ars.gov; youngnam@cnu.ac.kr; hyounlim@cnu.ac.kr FU Ministry of Agriculture, Food and Rural Affairs [112018-3, 113044-3] FX This research was supported by Agricultural Biotechnology Development Program (Project No. 112018-3) and Technology Commercialization Support Program (Project No. 113044-3), Ministry of Agriculture, Food and Rural Affairs. NR 36 TC 1 Z9 1 U1 2 U2 10 PU KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS PI FUKUOKA-SHI PA 6-10-1 HAKOZAKI, HIGASHI-KU, FUKUOKA-SHI, 812-0053, JAPAN SN 0023-6152 J9 J FAC AGR KYUSHU U JI J. Fac. Agric. Kyushu Univ. PD FEB PY 2015 VL 60 IS 1 BP 139 EP 149 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA CD8FD UT WOS:000351329700019 ER PT J AU Lee, S Lohumi, S Lim, HS Gotoh, T Cho, BK Kim, MS Lee, SH AF Lee, Sangdae Lohumi, Santosh Lim, Hyoun-Sub Gotoh, Takafumi Cho, Byoung-Kwan Kim, Moon S. Lee, Soo-Hee TI Development of a Detection Method for Adulterated Onion Powder using Raman Spectroscopy SO JOURNAL OF THE FACULTY OF AGRICULTURE KYUSHU UNIVERSITY LA English DT Article DE cornstarch; onion powder; adulteration; Raman spectroscopy; PLSR ID MILK; MELAMINE; OIL AB The purpose of this study was to develop a detection technique for adulterated powder products using Raman spectroscopy. While conventional methods for food quality analysis require time and pretreatment procedures, Raman spectroscopy can be used to detect a food component rapidly and nondestructively without pretreatment. In this study, Raman spectra were measured using a 785 nm laser source in the range 3,200 similar to 170 cm(-1) and multivariate analytical method using partial least square regression (PLSR) was applied to develop optimal models for predicting the mixed cornstarch concentration in adulterated onion powders. A range of preprocessing methods were applied to correct Raman spectra by reducing the systematic noise and variations produced by the light source and light scattering of the sample surface. Raman data were predicted with a high R-2 value and low standard error for the entire preprocessing data set. The method proved its effectiveness to rapidly detect adulteration in powdered food. C1 [Lee, Sangdae] Korea Inst Ind Technol, Convergence Components & Agr Machinery Applicat C, Gimje Si 576881, Jeollabuk Do, South Korea. [Lohumi, Santosh; Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Biosyst Machinery Engn, Taejon 305764, South Korea. [Lim, Hyoun-Sub] Chungnam Natl Univ, Dept Appl Biol, Taejon 305764, South Korea. [Gotoh, Takafumi] Kyushu Univ, Kuju Agr Res Ctr, Fac Agr, Taketa City, Oita 878020, Japan. [Kim, Moon S.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Lee, Soo-Hee] Life & Technol Co LtD, Jeongnammyeon 445964, Hwaseong Si, South Korea. RP Cho, BK (reprint author), Kyushu Univ, Kuju Agr Res Ctr, Kuju 4045-4, Taketa City, Oita 878020, Japan. EM chobk@cnu.ac.kr FU Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA); Next Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008055] FX This research was partially supported by Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA) and by a grant from the Next Generation BioGreen 21 Program (No. PJ008055), Rural Development Administration, Republic of Korea. NR 18 TC 0 Z9 0 U1 3 U2 7 PU KYUSHU UNIV, FACULTY AGRICULTURAL PUBLICATIONS PI FUKUOKA-SHI PA 6-10-1 HAKOZAKI, HIGASHI-KU, FUKUOKA-SHI, 812-0053, JAPAN SN 0023-6152 J9 J FAC AGR KYUSHU U JI J. Fac. Agric. Kyushu Univ. PD FEB PY 2015 VL 60 IS 1 BP 151 EP 156 PG 6 WC Agriculture, Multidisciplinary SC Agriculture GA CD8FD UT WOS:000351329700020 ER PT J AU Diaz-Lara, A Mosier, NJ Keller, KE Martin, RR AF Diaz-Lara, Alfredo Mosier, Nola J. Keller, Karen E. Martin, Robert R. TI A variant of Rubus yellow net virus with altered genomic organization SO VIRUS GENES LA English DT Article DE Badnavirus; Rubus species; New strain; Genomic rearrangement; Aphid transmission ID ROLLING-CIRCLE AMPLIFICATION; MOTTLE VIRUS; SEQUENCE; TRANSMISSION; PARARETROVIRUS; IDENTIFICATION; BADNAVIRUS; PROMOTER; DATABASE; PROTEIN AB Rubus yellow net virus (RYNV) is a member of the genus Badnavirus (family: Caulimoviridae). RYNV infects Rubus species causing chlorosis of the tissue along the leaf veins, giving an unevenly distributed netted symptom in some cultivars of red and black raspberry. Recently, a strain of RYNV was sequenced from a Rubus idaeus plant in Alberta, Canada, exhibiting such symptoms. The viral genome contained seven open reading frames (ORFs) with five of them in the sense-strand, including a large polyprotein. Here we describe a graft-transmissible strain of RYNV from Europe infecting cultivar 'Baumforth's Seedling A' (named RYNV-BS), which was sequenced using rolling circle amplification, enzymatic digestion, cloning and primer walking, and it was resequenced at a 5X coverage. This sequence was then compared with the RYNV-Ca genome and significant differences were observed. Genomic analysis identified differences in the arrangement of coding regions, promoter elements, and presence of motifs. The genomic organization of RYNV-BS consisted of five ORFs (four ORFs in the sense-strand and one ORF in the antisense-strand). ORFs 1, 2, and 3 showed a high degree of homology to RYNV-Ca, while ORFs 4 and 6 of RYNV-BS were quite distinct. Also, the predicted ORFs 5 and 7 in the RYNV-Ca were absent in the RYNV-BS sequence. These differences may account for the lack of aphid transmissibility of RYNV-BS. C1 [Diaz-Lara, Alfredo] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Mosier, Nola J.; Keller, Karen E.; Martin, Robert R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Diaz-Lara, A (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM diazlara@onid.orst.edu FU Northwest Center for Small Fruits Research; Washington Red Raspberry Commission; Oregon Raspberry and Blackberry Commission; USDA-NIFA-SCRI [2009-51181-06022] FX We thank Joseph Postman from the National Clonal Germplasm Repository-USDA (Corvallis, Oregon) for providing information and plant material used during this study. Also, we thank Javier F Tabima (Oregon State University) for his advice on phylogenetic analysis. The work presented here was partially funded with Grants from the Northwest Center for Small Fruits Research, the Washington Red Raspberry Commission, the Oregon Raspberry and Blackberry Commission, and USDA-NIFA-SCRI (2009-51181-06022). Diaz-Lara did most of the work reported here, with the other authors contributed equally to the work. NR 30 TC 2 Z9 2 U1 0 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD FEB PY 2015 VL 50 IS 1 BP 104 EP 110 DI 10.1007/s11262-014-1149-6 PG 7 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA CC9NK UT WOS:000350697300015 PM 25480633 ER PT J AU Rose, LA Sebestyen, SD Elliott, EM Koba, K AF Rose, Lucy A. Sebestyen, Stephen D. Elliott, Emily M. Koba, Keisuke TI Drivers of atmospheric nitrate processing and export in forested catchments SO WATER RESOURCES RESEARCH LA English DT Review DE nitrogen deposition; nitrate isotopes; catchment hydrology; nitrogen saturation ID NORTHERN HARDWOOD FOREST; OXYGEN-ISOTOPE RATIOS; NITROGEN DEPOSITION; TEMPERATE FOREST; RIPARIAN ZONES; ORGANIC-CARBON; UNITED-STATES; SURFACE-WATER; LONG-TERM; NEW-YORK AB Increased deposition of reactive atmospheric N has resulted in the nitrogen saturation of many forested catchments worldwide. Isotope-based studies from multiple forest sites report low proportions (mean=approximate to 10%) of unprocessed atmospheric nitrate in streams during baseflow, regardless of N deposition or nitrate export rates. Given similar proportions of atmospheric nitrate in baseflow across a variety of sites and forest types, it is important to address the postdepositional drivers and processes that affect atmospheric nitrate transport and fate within catchments. In a meta-analysis of stable isotope-based studies, we examined the influence of methodological, biological, and hydrologic drivers on the export of atmospheric nitrate from forests. The delta O-18-NO3- values in stream waters may increase, decrease, or not change with increasing discharge during stormflow conditions, and delta O-18-NO3- values are generally higher in stormflow than baseflow. However, delta O-18-NO3- values tended to increase with increasing baseflow discharge at all sites examined. To explain these differences, we present a conceptual model of hydrologic flowpath characteristics (e.g., saturation overland flow versus subsurface stormflow) that considers the influence of topography on landscape-stream hydrologic connectivity and delivery of unprocessed atmospheric nitrate to streams. Methodological biases resulting from differences in sampling frequency and stable isotope analytical techniques may further influence the perceived degree of unprocessed atmospheric nitrate export. Synthesis of results from numerous isotope-based studies shows that small proportions of unprocessed atmospheric nitrate are common in baseflow. However, hydrologic, topographic, and methodological factors are important drivers of actual or perceived elevated contributions of unprocessed atmospheric nitrate to streams. C1 [Rose, Lucy A.; Elliott, Emily M.] Univ Pittsburgh, Dept Geol & Planetary Sci, Pittsburgh, PA 15260 USA. [Sebestyen, Stephen D.] US Forest Serv, USDA, Grand Rapids, MN USA. [Koba, Keisuke] Tokyo Univ Agr & Technol, Inst Agr, Tokyo, Japan. RP Rose, LA (reprint author), Univ Pittsburgh, Dept Geol & Planetary Sci, Pittsburgh, PA 15260 USA. EM lab125@pitt.edu RI Koba, Keisuke/A-3699-2012; Sebestyen, Stephen/D-1238-2013; Elliott, Emily /E-8122-2011 OI Koba, Keisuke/0000-0003-1942-9811; Sebestyen, Stephen/0000-0002-6315-0108; Elliott, Emily /0000-0002-9653-1513 FU NSF [0910521, EAR-1256273]; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2012-67011-19663]; Japan Society for the Promotion of Science [GS008, 26252020] FX This work was supported by NSF grant 0910521 and EAR-1256273 to EME, Agriculture and Food Research Initiative grant 2012-67011-19663 from the USDA National Institute of Food and Agriculture to LAR, and NEXT Program (GS008) and KAKENHI Grant (26252020) of the Japan Society for the Promotion of Science to KK. We thank the participants of the US-Japan Joint Seminar on Responses of Catchment Hydrology and Forest Biogeochemistry to Climatic and Environmental Change, the workshop from which this manuscript originated. We also thank Chris Evans, Gary Lovett, Dan Bain, and three anonymous reviewers for helpful comments during the preparation of this manuscript. Data presented in the table and figures are available from the publications listed therein. NR 123 TC 4 Z9 4 U1 4 U2 39 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD FEB PY 2015 VL 51 IS 2 BP 1333 EP 1352 DI 10.1002/2014WR015716 PG 20 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA CD9ET UT WOS:000351401200031 ER PT J AU Nghiem, NP Senske, GE AF Nghiem, Nhuan P. Senske, Gerard E. TI Capture of Carbon Dioxide from Ethanol Fermentation by Liquid Absorption for Use in Biological Production of Succinic Acid SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE Ethanol fermentation; CO2 capture; Biorefinery; Succinic acid; Escherichia coli AFP184 AB Previously, it was shown that the gas produced in an ethanol fermentor using either corn or barley as feedstock could be sparged directly into an adjacent fermentor as a feedstock for succinic acid fermentation using Escherichia coli AFP184. In the present investigation, it was demonstrated that the CO2 produced in a corn ethanol fermentor could be absorbed in a base solution and the resultant carbonate solution used both for pH control and supply of the CO2 requirement in succinic acid fermentation. Thus, the CO2 produced in a 5-L corn mash containing 30 wt% total solids was absorbed in a packed column containing 2 L of either 5 M NaOH, 5 M KOH, or 15 wt% NH4OH, and the resultant carbonate solutions were used for pH control in a succinic acid fermentor. The results obtained indicated no significant differences between succinic acid production in these experiments and when 2.5 M solutions of Na2CO3, K2CO3, and (NH4)(2)CO3 from commercial sources were used. In a commercial setting, the demonstrated capture of CO2 in liquid form will allow transportation of the carbonate solutions to locations not in the immediate vicinity of the ethanol plant, and excess carbonate salts can also be recovered as value-added products. C1 [Nghiem, Nhuan P.; Senske, Gerard E.] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Nghiem, NP (reprint author), ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM john.nghiem@ars.usda.gov NR 19 TC 2 Z9 2 U1 3 U2 22 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 EI 1559-0291 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD FEB PY 2015 VL 175 IS 4 BP 2104 EP 2113 DI 10.1007/s12010-014-1369-1 PG 10 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA CD2BN UT WOS:000350878700026 PM 25448631 ER PT J AU Maki, EC Cohnstaedt, LW AF Maki, Elin C. Cohnstaedt, Lee W. TI Crowdsourcing for large-scale mosquito (Diptera: Culicidae) sampling SO CANADIAN ENTOMOLOGIST LA English DT Article ID WEST-NILE-VIRUS; VECTOR COMPETENCE AB Sampling a cosmopolitan mosquito (Diptera: Culicidae) species throughout its range is logistically challenging and extremely resource intensive. Mosquito control programmes and regional networks operate at the local level and often conduct sampling activities across much of North America. A method for large-scale sampling of two mosquito species using crowdsourcing to network with these local and regional programmes is described. A total of 961 mosquito vector and control districts, health departments, and individual collectors across the United States of America and Canada were contacted in 2011 and 2012 of which 9% positively responded by sending mosquitoes. In total, 1101 unique population samples of Aedes vexans (Meigen) and Culex tarsalis Coquillett were collected throughout their range in these two countries. Aedes vexans outgroup samples were also submitted from Europe and Asia. This is the largest crowd-sourced collection of samples to date. C1 [Maki, Elin C.; Cohnstaedt, Lee W.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS 66502 USA. RP Cohnstaedt, LW (reprint author), ARS, Ctr Grain & Anim Hlth Res, USDA, 1515 Coll Ave, Manhattan, KS 66502 USA. EM Lee.Cohnstaedt@ars.usda.gov NR 8 TC 0 Z9 0 U1 2 U2 6 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD FEB PY 2015 VL 147 IS 1 BP 118 EP 123 DI 10.4039/tce.2014.27 PG 6 WC Entomology SC Entomology GA CD2AB UT WOS:000350874700011 ER PT J AU Gutierrez, J Gribok, A Rumpler, W Chandran, A DiPietro, L AF Gutierrez, Jean Gribok, Andrei Rumpler, William Chandran, Avinash DiPietro, Loretta TI A Single Bout of Resistance Exercise Does Not Promote Excess Postexercise Energy Expenditure in Untrained Young Men with a Family History of Diabetes SO INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM LA English DT Article DE resistance training; strength training; metabolism; EPOC; EPEE; oxygen consumption ID RESTING METABOLIC-RATE; OXYGEN-CONSUMPTION; INSULIN SENSITIVITY; GENETIC-VARIANTS; INTENSITY; DURATION; RISK; EPOC AB Background: People with a family history of type 2 diabetes have lower energy expenditure (EE) and more obesity than those having no such family history. Resistance exercise (RE) may induce excess postexercise energy expenditure (EPEE) and reduce long-term risk for obesity in this susceptible group. Purpose: To determine the effect of RE on EPEE for 15 hr after a single exercise bout in healthy, untrained young men having a family history of type 2 diabetes. Design: Seven untrained men (23 +/- 1.2 years, BMI 24 +/- 1.1) completed a 48-hr protocol in a whole room calorimeter. The first day served as a control day, with a moderate 40-min RE bout occurring on the second day. Differences in postexercise EE were compared with matched periods from the control day for cumulative 15-min intervals (up to 150 min) and 15 hr after the RE bout was completed. Results: The most robust difference in EPEE between the experimental and control days was observed in the first 15-min postexercise period (M = 1.4Kcal/min; SD = 0.7; p < .05). No statistically significant differences in EPEE were noted beyond 90-min of continuous measurement. Conclusions: Young people with a family history of type 2 diabetes may not show EPEE after a single RE bout when observed for 15 hr after RE and long-term resistance training may be required to promote EPEE. C1 [Gutierrez, Jean; Chandran, Avinash; DiPietro, Loretta] George Washington Univ, Sch Publ Hlth & Hlth Serv, Dept Exercise Sci, Washington, DC 20052 USA. [Gribok, Andrei; Rumpler, William] USDA, Beltsville Human Nutr Res Lab, Beltsville, MD 20705 USA. RP Gutierrez, J (reprint author), George Washington Univ, Sch Publ Hlth & Hlth Serv, Dept Exercise Sci, Washington, DC 20052 USA. EM jeanguti@utmb.edu NR 28 TC 0 Z9 0 U1 1 U2 1 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1526-484X EI 1543-2742 J9 INT J SPORT NUTR EXE JI Int. J. Sport Nutr. Exerc. Metab. PD FEB PY 2015 VL 25 IS 1 BP 20 EP 26 DI 10.1123/ijsnem.2013-0244 PG 7 WC Nutrition & Dietetics; Sport Sciences SC Nutrition & Dietetics; Sport Sciences GA CD0PS UT WOS:000350777000003 PM 24901339 ER PT J AU Gibson, DM Vaughan, RH Milbrath, LR AF Gibson, Donna M. Vaughan, Richard H. Milbrath, Lindsey R. TI Invasive Swallow-worts: An Allelopathic Role for -(-) Antofine Remains Unclear SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE -(-) Antofine; Phytotoxin; Swallow-worts; Vincetoxicum; Cynanchum; Allelopathy; Invasiveness ID VINE VINCETOXICUM-ROSSICUM; NEW-YORK-STATE; SPOTTED KNAPWEED; ALLELOCHEMICAL (+/-)-CATECHIN; PHRAGMITES-AUSTRALIS; CYNANCHUM-ROSSICUM; V. NIGRUM; PLANTS; SUCCESS; GROWTH AB Pale swallow-wort (Vincetoxicum rossicum) and black swallow-wort (V. nigrum) are two invasive plant species in the northeastern United States and eastern Canada that have undergone rapidly expanding ranges over the past 30 years. Both species possess a highly bioactive phytotoxin -(-) antofine in root tissues that causes pronounced inhibition in laboratory bioassays of native plant species co-located in habitats where swallow-wort is found. To further evaluate the allelopathic potential of -(-) antofine, we: determined its concentration in young plant tissues; used in situ approaches to assess antofine stability, potential activity of degradation products, activity in sterile and nonsterile soil; and determined accumulation and concentration in hydroponic cultivation and field collected soil samples. Extracts of seeds and young seedlings were found to have approximately 2-3 times the level of -(-) antofine in comparison to root extracts of adult plants. Breakdown products of antofine accumulated rapidly with exposure to light, but more slowly in the dark, at ambient temperatures, and these products did not retain biological activity. Extraction efficiencies of control soil spiked with -(-) antofine were low but easily detectable by HPLC. Soil samples collected over two growing seasons at four different sites where either pale swallow-wort or black swallow-wort populations are present were negative for the presence of -(-) antofine. Dose response curves using sterile and nonsterile soil spiked with -(-) antofine demonstrated a requirement for at least 20-55 x greater -(-) antofine concentrations in soil to produce similar phytotoxic effects to those previously seen in agar bioassays with lettuce seedlings. Sterile soil had a calculated EC50 of 686 mu M (250 mu g/g) as compared to nonsterile soil treatments with a calculated EC50 of 1.88 mM (640 mu g/g). When pale swallow-wort and black swallow-wort adult plants were grown in hydroponic cultivation, -(-) antofine was found in root exudates and in the growing medium in the nM range. The concentrations in exudate were much lower than that needed for biological activity (mu M) although they might be an underestimate of what may accumulate over time in an undisturbed rhizosphere. Based on these various results, it remains uncertain as to whether -(-) antofine could play a significant allelopathic role for invasive swallow-worts. C1 [Gibson, Donna M.; Vaughan, Richard H.; Milbrath, Lindsey R.] ARS, USDA, Biol Integrated Pest Management Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Milbrath, LR (reprint author), ARS, USDA, Biol Integrated Pest Management Res Unit, Robert W Holley Ctr Agr & Hlth, Tower Rd, Ithaca, NY 14853 USA. EM Lindsey.Milbrath@ars.usda.gov NR 52 TC 1 Z9 1 U1 2 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD FEB PY 2015 VL 41 IS 2 BP 202 EP 211 DI 10.1007/s10886-015-0552-3 PG 10 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA CD2DZ UT WOS:000350885700010 PM 25653045 ER PT J AU Salinas, RA Stiver, WH Corn, JL Lenhart, S Collins, C Madden, M Vercauteren, KC Schmit, BB Kasari, E Odoi, A Hickling, G Mccallum, H AF Salinas, Rene A. Stiver, William H. Corn, Joseph L. Lenhart, Suzanne Collins, Charles Madden, Marguerite Vercauteren, Kurt C. Schmit, Brandon B. Kasari, Ellen Odoi, Agricola Hickling, Graham Mccallum, Hamish TI AN INDIVIDUAL-BASED MODEL FOR FERAL HOGS IN GREAT SMOKY MOUNTAINS NATIONAL PARK SO NATURAL RESOURCE MODELING LA English DT Article DE Sus scrofa; Eurasian wild boar; invasive species; individual-based model; feral hog; hard mast ID EUROPEAN WILD HOGS; PROTOCOL; FOODS; PIGS AB The expansion of feral hog (Sus scrofa) populations in the United States has resulted in increased efforts to develop and implement control strategies designed to minimize the impacts done by this invasive species. We describe an individual-based model for feral hogs in Great Smoky Mountains National Park (GSMNP). The objectives of the model are to provide an understanding of the population dynamics of this feral hog population and to determine the efficacy of the annual harvest as a population control method. Results suggest that the dynamics of the population are driven by fall hard mast production and the GSMNP harvests currently limit growth of the population, but these control efforts have not reduced the population. C1 [Salinas, Rene A.] Appalachian State Univ, Dept Math Sci, Boone, NC 28608 USA. [Stiver, William H.] Natl Pk Serv, US Dept Interior, Gatlinburg, TN 37738 USA. [Corn, Joseph L.] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Lenhart, Suzanne; Collins, Charles] Univ Tennessee, Dept Math, Knoxville, TN 37996 USA. [Madden, Marguerite] Univ Georgia, Dept Geog, Ctr Remote Sensing & Mapping Sci, Athens, GA 30602 USA. [Vercauteren, Kurt C.] APHIS Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Schmit, Brandon B.] APHIS Wildlife Serv, USDA, Natl Wildlife Dis Program, Ft Collins, CO 80521 USA. [Kasari, Ellen] APHIS Vet Serv, USDA, Ft Collins, CO 80521 USA. [Odoi, Agricola] Univ Tennessee, Dept Clin Sci, Knoxville, TN 37996 USA. [Hickling, Graham] Univ Tennessee, Dept Forestry, Ctr Wildlife Hlth Wildlife & Fisheries, Knoxville, TN 37996 USA. [Mccallum, Hamish] Griffith Univ, Sch Environm, Nathan, Qld 4111, Australia. RP Salinas, RA (reprint author), Appalachian State Univ, Dept Math Sci, Boone, NC 28608 USA. EM salinasra@appstate.edu RI McCallum, Hamish/E-1638-2013; OI McCallum, Hamish/0000-0002-3493-0412; Odoi, Agricola/0000-0001-5050-6737 FU National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF [EF-0832858]; University of Tennessee, Knoxville FX This work is part of a Working Group at the National Institute for Mathematical and Biological Synthesis, an Institute sponsored by the National Science Foundation, the U.S. Department of Homeland Security, and the U.S. Department of Agriculture through NSF Award EF-0832858, with additional support from The University of Tennessee, Knoxville. NR 28 TC 2 Z9 2 U1 6 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0890-8575 EI 1939-7445 J9 NAT RESOUR MODEL JI Nat. Resour. Model. PD FEB PY 2015 VL 28 IS 1 BP 18 EP 36 DI 10.1111/nrm.12055 PG 19 WC Environmental Sciences; Mathematics, Interdisciplinary Applications SC Environmental Sciences & Ecology; Mathematics GA CC4UK UT WOS:000350349200002 ER PT J AU Marcot, BG Singleton, PH Schumaker, NH AF Marcot, Bruce G. Singleton, Peter H. Schumaker, Nathan H. TI ANALYSIS OF SENSITIVITY AND UNCERTAINTY IN AN INDIVIDUAL-BASED MODEL OF A THREATENED WILDLIFE SPECIES SO NATURAL RESOURCE MODELING LA English DT Article DE Individual-based model; HexSim model; sensitivity analysis; uncertainty analysis ID NORTHERN SPOTTED OWLS; POPULATION-DYNAMICS; BARRED OWL; PATCH SIZE; VIABILITY; LANDSCAPE; DISPERSAL; CONSERVATION; CALIBRATION; PARAMETERS AB Sensitivity analysisdetermination of how prediction variables affect response variablesof individual-based models (IBMs) are few but important to the interpretation of model output. We present sensitivity analysis of a spatially explicit IBM (HexSim) of a threatened species, the Northern Spotted Owl (NSO; Strix occidentalis caurina) in Washington, USA. We explored sensitivity to HexSim variables representing habitat quality, movement, dispersal, and model architecture; previous NSO studies have well established sensitivity of model output to vital rate variation. We developed normative (expected) model settings from field studies, and then varied the values of 1 input parameter at a time by +/- 10% and +/- 50% of their normative values to determine influence on response variables of population size and trend. We determined time to population equilibration and dynamics of populations above and below carrying capacity. Recovery time from small population size to carrying capacity greatly exceeded decay time from an overpopulated condition, suggesting lag time required to repopulate newly available habitat. Response variables were most sensitive to input parameters of habitat quality which are well-studied for this species and controllable by management. HexSim thus seems useful for evaluating potential NSO population responses to landscape patterns for which good empirical information is available. C1 [Marcot, Bruce G.] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97208 USA. [Singleton, Peter H.] US Forest Serv, USDA, Pacific NW Res Stn, Wenatchee, WA 98801 USA. [Schumaker, Nathan H.] US EPA, Environm Res Lab, Corvallis, OR 97333 USA. RP Marcot, BG (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 620 SW Main St,Suite 400, Portland, OR 97208 USA. EM cot@fs.fed.us; psingleton@fs.fed.us; schumaker.nathan@epa.gov FU Joint Fire Science Program of U.S. Forest Service and Bureau of Land Management; U.S. Environmental Protection Agency (EPA) FX This work is part of a broader project funded under the Joint Fire Science Program of U.S. Forest Service and Bureau of Land Management. We thank other members of our project team for discussions and support, including E. Haunreiter, P. Hessburg, R. Kennedy, J. Lehmkuhl, M. Raphael, R. Scheller, and T. Spies. We also thank the members of the U.S. Fish and Wildlife Service's Northern Spotted Owl Modeling Team for inspiration. Programmers A. Brookes and K. Djang provided the code used for HexSim. The information in this document has been funded in part by the U.S. Environmental Protection Agency (EPA). It has been subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division, EPA, and approved for publication. Approval does not signify that the contents reflect the views of EPA, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. We thank Jeff Dunk, Chad Wilsey, and three anonymous reviewers for comments on the paper. NR 55 TC 5 Z9 5 U1 3 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0890-8575 EI 1939-7445 J9 NAT RESOUR MODEL JI Nat. Resour. Model. PD FEB PY 2015 VL 28 IS 1 BP 37 EP 58 DI 10.1111/nrm.12056 PG 22 WC Environmental Sciences; Mathematics, Interdisciplinary Applications SC Environmental Sciences & Ecology; Mathematics GA CC4UK UT WOS:000350349200003 ER PT J AU Asseng, S Ewert, F Martre, P Rotter, RP Lobell, DB Cammarano, D Kimball, BA Ottman, MJ Wall, GW White, JW Reynolds, MP Alderman, PD Prasad, PVV Aggarwal, PK Anothai, J Basso, B Biernath, C Challinor, AJ De Sanctis, G Doltra, J Fereres, E Garcia-Vile, M Gayler, S Hoogenboom, G Hunt, LA Izaurralde, RC Jabloun, M Jones, CD Kersebaum, KC Koehler, AK Muller, C Kumar, SN Nendel, C O'Leary, G Olesen, JE Palosuo, T Priesack, E Rezaei, EE Ruane, AC Semenov, MA Shcherbak, I Stockle, C Stratonovitch, P Streck, T Supit, I Tao, F Thorburn, PJ Waha, K Wang, E Wallach, D Wolf, I Zhao, Z Zhu, Y AF Asseng, S. Ewert, F. Martre, P. Roetter, R. P. Lobell, D. B. Cammarano, D. Kimball, B. A. Ottman, M. J. Wall, G. W. White, J. W. Reynolds, M. P. Alderman, P. D. Prasad, P. V. V. Aggarwal, P. K. Anothai, J. Basso, B. Biernath, C. Challinor, A. J. De Sanctis, G. Doltra, J. Fereres, E. Garcia-Vile, M. Gayler, S. Hoogenboom, G. Hunt, L. A. Izaurralde, R. C. Jabloun, M. Jones, C. D. Kersebaum, K. C. Koehler, A-K. Mueller, C. Kumar, S. Naresh Nendel, C. O'Leary, G. Olesen, J. E. Palosuo, T. Priesack, E. Rezaei, E. Eyshi Ruane, A. C. Semenov, M. A. Shcherbak, I. Stoeckle, C. Stratonovitch, P. Streck, T. Supit, I. Tao, F. Thorburn, P. J. Waha, K. Wang, E. Wallach, D. Wolf, I. Zhao, Z. Zhu, Y. TI Rising temperatures reduce global wheat production SO NATURE CLIMATE CHANGE LA English DT Article ID CLIMATE-CHANGE; SPRING WHEAT; DRYLAND WHEAT; YIELD; GROWTH; DROUGHT; HEAT; CO2; AGRICULTURE; ADAPTATION AB Crop models are essential tools for assessing the threat of climate change to local and global food production(1). Present models used to predict wheat grain yield are highly uncertain when simulating how crops respond to temperature(2). Here we systematically tested 30 different wheat crop models of the Agricultural Model Intercomparison and Improvement Project against field experiments in which growing season mean temperatures ranged from 15 degrees C to 32 degrees C, including experiments with artificial heating. Many models simulated yields well, but were less accurate at higher temperatures. The model ensemble median was consistently more accurate in simulating the crop temperature response than any single model, regardless of the input information used. Extrapolating the model ensemble temperature response indicates that warming is already slowing yield gains at a majority of wheat-growing locations. Global wheat production is estimated to fall by 6% for each degrees C of further temperature increase and become more variable over space and time. C1 [Asseng, S.; Cammarano, D.] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Ewert, F.; Rezaei, E. Eyshi] Univ Bonn, Inst Crop Sci & Resource Conservat INRES, D-53115 Bonn, Germany. [Martre, P.; Kimball, B. A.] INRA, UMR Genet Divers & Ecophysiol Cereales GDEC 1095, F-63100 Clermont Ferrand, France. [Martre, P.] Univ Blaise Pascal, GDEC UMR1095, F-63170 Aubiere, France. [Roetter, R. P.; Palosuo, T.; Tao, F.] MTT Agr Res Finland, Plant Prod Res, FI-50100 Mikkeli, Finland. [Lobell, D. B.] Stanford Univ, Dept Environm Earth Syst Sci, Stanford, CA 94305 USA. [Lobell, D. B.] Stanford Univ, Ctr Food Secur & Environm, Stanford, CA 94305 USA. [Kimball, B. A.; Wall, G. W.; White, J. W.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Ottman, M. J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Reynolds, M. P.; Alderman, P. D.] CIMMYT Int Adpo, Mexico City 06600, DF, Mexico. [Prasad, P. V. V.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Aggarwal, P. K.] Int Water Management Inst, CGIAR Res Program Climate Change Agr & Food Secur, New Delhi 110012, India. [Anothai, J.; Hoogenboom, G.] Washington State Univ, Biol Syst Engn, Prosser, WA 99350 USA. [Basso, B.; Shcherbak, I.] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48823 USA. [Basso, B.; Shcherbak, I.] Michigan State Univ, WK Kellogg Biol Stn, E Lansing, MI 48823 USA. [Biernath, C.; Priesack, E.] Helmholtz Zentrum Munchen, German Res Ctr Environm Hlth, Inst Soil Ecol, D-85764 Neuherberg, Germany. [Challinor, A. J.; Koehler, A-K.] Univ Leeds, Inst Climate & Atmospher Sci, Sch Earth & Environm, Leeds LS2 9JT, W Yorkshire, England. [Challinor, A. J.] CIAT, CGIAR ESSP Program Climate Change Agr & Food Secu, Cali 6713, Colombia. [De Sanctis, G.] INRA, AgroClim US1116, F-84914 Avignon, France. [Doltra, J.] Cantabrian Agr Res & Training Ctr CIFA, Muriedas 39600, Spain. [Fereres, E.; Garcia-Vile, M.] IAS CSIC, Cordoba 14080, Spain. [Fereres, E.; Garcia-Vile, M.] Univ Cordoba, E-14080 Cordoba, Spain. [Gayler, S.] Univ Tubingen, WESS Water & Earth Syst Sci Competence Cluster, D-72074 Tubingen, Germany. [Hunt, L. A.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Izaurralde, R. C.; Jones, C. D.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Izaurralde, R. C.] Texas A&M Univ, Texas A&M AgriLife Res & Extens Ctr, Temple, TX 76502 USA. [Jabloun, M.; Olesen, J. E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Kersebaum, K. C.; Nendel, C.] Leibniz Ctr Agr Landscape Res, Inst Landscape Syst Anal, D-15374 Muncheberg, Germany. [Mueller, C.; Waha, K.] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [Kumar, S. Naresh] IARI PUSA, Indian Agr Res Inst, Ctr Environm Sci & Climate Resilient Agr, New Delhi 110012, India. [O'Leary, G.] Dept Environm & Primary Ind, Landscape & Water Sci, Horsham, Vic 3400, Australia. [Ruane, A. C.] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Semenov, M. A.; Stratonovitch, P.] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Stoeckle, C.] Washington State Univ, Biol Syst Engn, Pullman, WA 99164 USA. [Streck, T.] Univ Hohenheim, Inst Soil Sci & Land Evaluat, D-70599 Stuttgart, Germany. [Supit, I.; Wolf, I.] Wageningen Univ, Plant Prod Syst & Earth Syst Sci, NL-6700 AA Wageningen, Netherlands. [Tao, F.] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Thorburn, P. J.] CSIRO Agr Flagship, Dutton Pk, Qld 4102, Australia. [Wang, E.; Zhao, Z.] CSIRO Agr Flagship, Black Mt, ACT 2601, Australia. [Wallach, D.] INRA, UMR Agrosyst & Dev Terr AGIR 1248, F-31326 Castanet Tolosan, France. [Zhao, Z.] China Agr Univ, Dept Agron & Biotechnol, Beijing 100193, Peoples R China. [Zhu, Y.] Nanjing Agr Univ, Coll Agr, Nanjing 210095, Jiangsu, Peoples R China. RP Asseng, S (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM sasseng@ufl.edu RI Garcia-Vila, Margarita/N-6805-2015; Palosuo, Taru/B-9593-2012; Doltra, Jordi/C-2106-2015; Thorburn, Peter/A-6884-2011; Challinor, Andrew/C-4992-2008; Wang, Enli/K-7478-2012; Priesack, Eckart/M-7341-2014; Martre, Pierre/G-5399-2013; Zhao, Zhigan/E-8963-2015; Mueller, Christoph/E-4812-2016; Olesen, Jorgen/C-2905-2016 OI Wallach, Daniel/0000-0003-3500-8179; Priesack, Eckart/0000-0002-5088-9528; Jabloun, Mohamed/0000-0003-1199-1316; Stratonovitch, Pierre/0000-0002-5806-2066; Reynolds, Matthew Paul/0000-0002-4291-4316; Cammarano, Davide/0000-0003-0918-550X; Eyshi Rezaei, Ehsan/0000-0003-2603-8034; Garcia-Vila, Margarita/0000-0001-5737-4669; Palosuo, Taru/0000-0003-4322-3450; Kersebaum, Kurt Christian/0000-0002-3679-8427; Challinor, Andrew/0000-0002-8551-6617; Wang, Enli/0000-0002-6653-5791; Martre, Pierre/0000-0002-7419-6558; Zhao, Zhigan/0000-0003-1533-7215; Mueller, Christoph/0000-0002-9491-3550; Olesen, Jorgen/0000-0002-6639-1273 FU International Food Policy Research Institute (IFPRI); USDA National Institute for Food and Agriculture [32011-68002-30191]; KULUNDA [01LL0905L]; FACCE MACSUR project through the German FederalMinistry of Education and Research (BMBF) [031A103B, 2812ERA115]; German Science Foundation [EW119/5-1]; FACCEMACSUR project by the Danish Strategic Research Council; FACCE MACSUR project through the German Federal Ministry of Food and Agriculture (BMEL); FACCE MACSUR project funded through the Finnish Ministry of Agriculture and Forestry; National Natural Science Foundation of China [41071030]; Helmholtz project 'REKLIM-Regional Climate Change: Causes and Effects' Topic 9: 'Climate Change and Air Quality'; CGIAR Research Program on Climate Change, Agriculture, and Food Security (CCAFS); Australian Grains Research and Development Corporation; Department of Environment and Primary Industries Victoria, Australia; Texas AgriLife Research, Texas AM University; CSIRO; Chinese Academy of Sciences (CAS) FX We thank the Agricultural Model Intercomparison and Improvement Project and its leaders C. Rosenzweig from NASA Goddard Institute for Space Studies and Columbia University (USA), J. Jones from University of Florida (USA), J. Hatfield from United States Department of Agriculture (USA) and J. Antle from Oregon State University (USA) for support. We also thank M. Lopez from CIMMYT (Turkey), M. Usman Bashir from University of Agriculture, Faisalabad (Pakistan), S. Soufizadeh from Shahid Beheshti University (Iran), and J. Lorgeou and J-C. Deswarte from ARVALIS-Institut du Vegetal (France) for assistance with selecting key locations and quantifying regional crop cultivars, anthesis and maturity dates and R. Raymundo for assistance with GIS. S. A. and D. C. received financial support from the International Food Policy Research Institute (IFPRI). C. S. was funded through USDA National Institute for Food and Agriculture award 32011-68002-30191. C. M. received financial support from the KULUNDA project (01LL0905L) and the FACCE MACSUR project (031A103B) funded through the German FederalMinistry of Education and Research (BMBF). F.E. received support from the FACCE MACSUR project (031A103B) funded through the German Federal Ministry of Education and Research (2812ERA115) and E.E.R. was funded through the German Science Foundation (project EW119/5-1). M. J. and J.E.O. were funded through the FACCEMACSUR project by the Danish Strategic Research Council. K.C.K. and C.N. were funded by the FACCE MACSUR project through the German Federal Ministry of Food and Agriculture (BMEL). F.T., T.P. and R.P.R. received financial support from FACCE MACSUR project funded through the Finnish Ministry of Agriculture and Forestry (MMM); F.T. was also funded through National Natural Science Foundation of China (No. 41071030). C.B. was funded through the Helmholtz project 'REKLIM-Regional Climate Change: Causes and Effects' Topic 9: 'Climate Change and Air Quality'. M.P.R. and P.D.A. received funding from the CGIAR Research Program on Climate Change, Agriculture, and Food Security (CCAFS). G.O'L. was funded through the Australian Grains Research and Development Corporation and the Department of Environment and Primary Industries Victoria, Australia. R.C.I. was funded by Texas AgriLife Research, Texas A&M University. E.W. and Z.Z. were funded by CSIRO and the Chinese Academy of Sciences (CAS) through the research project 'Advancing crop yield while reducing the use of water and nitrogen' and by the CSIRO-MoE PhD Research Program. NR 29 TC 102 Z9 103 U1 68 U2 256 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD FEB PY 2015 VL 5 IS 2 BP 143 EP 147 DI 10.1038/NCLIMATE2470 PG 5 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA CC4MO UT WOS:000350327700025 ER PT J AU Dubey, JP Hilali, M Van Wilpe, E Verma, SK Calero-Bernal, R Abdel-Wahab, A AF Dubey, J. P. Hilali, M. Van Wilpe, E. Verma, S. K. Calero-Bernal, R. Abdel-Wahab, A. TI Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis) SO PARASITOLOGY LA English DT Article DE Sarcocystis fusiformis; sarcocyst; water buffalo (Bubalus bubalis); electron microscopy; ultrastructure ID PROTOZOA SARCOCYSTIDAE; SP. PROTOZOA; LEVINEI; ULTRASTRUCTURE; MALAYSIA; VIETNAM; SHEEP AB Four valid species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis, Sarcocystis buffalonis, Sarcocystis levinei and Sarcocystis dubeyi. Here, we redescribe structure of S. fusiformis sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9 mu m thick, depending on the section and the technique. In 5 mu m paraffin-embedded sections, the sarcocyst wall was indistinct, 2-5 mu m thick and appeared smooth. In 1 mu m plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2.5-5.2 mu m thick and had branched villar protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the sarcocyst wall. On each vp there were uniform papillomatous structures that were 100 nm wide. By TEM, vp were up to 6 mu m long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11.2 to 16.8 mu m in length. By TEM, bradyzoites had a very long (10 mu m) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of S. fusiformis from similar sarcocysts in domestic and wild ruminants. C1 [Dubey, J. P.; Verma, S. K.; Calero-Bernal, R.] USDA, ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Hilali, M.; Abdel-Wahab, A.] Cairo Univ, Fac Vet Med, Dept Parasitol, Giza 12211, Egypt. [Van Wilpe, E.] Univ Pretoria, Fac Vet Sci, Dept Anat & Physiol, ZA-0110 Onderstepoort, South Africa. RP Dubey, JP (reprint author), USDA, ARS, APDL, BARC East, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov RI Hilali, Mosaad/O-9443-2016; OI A. Hilali, Mosaad/0000-0002-9930-8501 FU Department of Employment and Innovation of the Regional Government of Extremadura (Spain) [PO12010]; European Social Fund FX R. Calero-Bernal is a postdoctoral fellow (ref. PO12010) funded by the Department of Employment and Innovation of the Regional Government of Extremadura (Spain) and the European Social Fund. NR 18 TC 5 Z9 5 U1 0 U2 3 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD FEB PY 2015 VL 142 IS 2 BP 385 EP 394 DI 10.1017/S003118201400122X PG 10 WC Parasitology SC Parasitology GA CD1AY UT WOS:000350807200011 PM 25111676 ER PT J AU Birge, HE Conant, RT Follett, RF Haddix, ML Morris, SJ Snapp, SS Wallenstein, MD Paul, EA AF Birge, Hannah E. Conant, Richard T. Follett, Ronald F. Haddix, Michelle L. Morris, Sherri J. Snapp, Sieglinde S. Wallenstein, Matthew D. Paul, Eldor A. TI Soil respiration is not limited by reductions in microbial biomass during long-term soil incubations SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Soil organic matter (SOM); Respiration; Microbial biomass; Incubation; Extracellular enzymes; Substrate decomposition ID ORGANIC-MATTER; GRASSLAND SOILS; CARBON; DYNAMICS; MINERALIZATION; FRACTIONATION; TEMPERATURE; MECHANISMS; TURNOVER; SIZE AB Declining rates of soil respiration are reliably observed during long-term laboratory incubations. However, the cause of this decline is uncertain. We explored different controls on soil respiration to elucidate the drivers of respiration rate declines during long-term soil incubations. Following a long-term (707 day) incubation (30 degrees C) of soils from two sites (a cultivated and a forested plot at Kellogg Biological Station, Hickory Corners, MI, USA), soils were significantly depleted of both soil carbon and microbial biomass. To test the ability of these carbon- and biomass-depleted ("incubation-depleted") soils to respire labile organic matter, we exposed soils to a second, 42 day incubation (30 degrees C) with and without an addition of plant residues. We controlled for soil carbon and microbial biomass depletion by incubating field fresh ("fresh") soils with and without an amendment of wheat and corn residues. Although respiration was consistently higher in the fresh versus incubation-depleted soil (2 and 1.2 times higher in the fresh cultivated and fresh forested soil, respectively), the ability to respire substrate did not differ between the fresh and incubation-depleted soils. Further, at the completion of the 42 day incubation, levels of microbial biomass in the incubation-depleted soils remained unchanged, while levels of microbial biomass in the field-fresh soil declined to levels similar to that of the incubation-depleted soils. Extra-cellular enzyme pools in the incubation-depleted soils were sometimes slightly reduced and did not respond to addition of labile substrate and did not limit soil respiration. Our results support the idea that available soil organic matter, rather than a lack microbial biomass and extracellular enzymes, limits soil respiration over the course of long-term incubations. That decomposition of both wheat and corn straw residues did not change after major changes in the soil biomass during extended incubation supports the omission of biomass values from biogeochemical models. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Birge, Hannah E.; Conant, Richard T.; Haddix, Michelle L.; Wallenstein, Matthew D.; Paul, Eldor A.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Nat Resources Ecol Lab, Ft Collins, CO 80523 USA. [Follett, Ronald F.] USDA ARS, Soil Plant Nutrient Res, Ft Collins, CO 80526 USA. [Morris, Sherri J.] Bradley Univ, Dept Biol, Peoria, IL 61625 USA. [Snapp, Sieglinde S.] Michigan State Univ, Dept Crop & Soil Sci, E Lansing, MI 48824 USA. RP Birge, HE (reprint author), 3310 Holdrege St,Hardin Hall, Lincoln, NE 68583 USA. EM hannah.birge@huskers.unl.edu RI Wallenstein, Matthew/C-6441-2008; Conant, Richard/B-7586-2013; OI Wallenstein, Matthew/0000-0002-6219-1442; Conant, Richard/0000-0001-7315-2476; Haddix, Michelle/0000-0003-0984-0404 FU Office of Science (BER), U.S. Department of Energy grant: Ecosystem Controls on Carbon and Nitrogen Sequestration Following Afforestation of Agricultural Soils [DE-FG03-01ER63183, DE-FG02-04ER63890]; National Science Foundation, Division of Environmental Biology [DEB-0842315]; US National Science Foundation Long Term Ecological Research Program [DEB 1027253] FX This research was supported by the Office of Science (BER), U.S. Department of Energy grant: Ecosystem Controls on Carbon and Nitrogen Sequestration Following Afforestation of Agricultural Soils (DE-FG03-01ER63183 and DE-FG02-04ER63890), The National Science Foundation, Division of Environmental Biology (DEB-0842315); and the US National Science Foundation Long Term Ecological Research Program (DEB 1027253). Our deep appreciation goes to two anonymous reviewers for their important suggestions and critical recommendations. NR 36 TC 3 Z9 3 U1 12 U2 73 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD FEB PY 2015 VL 81 BP 304 EP 310 DI 10.1016/j.soilbio.2014.11.028 PG 7 WC Soil Science SC Agriculture GA CC7CG UT WOS:000350524700037 ER PT J AU Sun, ZB Kuang, WJ AF Sun, Zhibin Kuang, Weijia TI An Ensemble Algorithm Based Component for Geomagnetic Data Assimilation SO TERRESTRIAL ATMOSPHERIC AND OCEANIC SCIENCES LA English DT Article; Proceedings Paper CT Academic Workshop on Earth Sciences CY NOV 18-20, 2012 CL Acad Sinica, Taipei, TAIWAN HO Acad Sinica DE Geomagnetic data assimilation; Ensemble algorithm; Geodynamo ID SECULAR VARIATION; MHD SYSTEM; FIELD; MODEL AB Geomagnetic data assimilation is one of the most recent developments in geomagnetic studies. It combines geodynamo model outputs and surface geomagnetic observations to provide more accurate estimates of the core dynamic state and provide accurate geomagnetic secular variation forecasting. To facilitate geomagnetic data assimilation studies, we develop a stand-alone data assimilation component for the geomagnetic community This component is used to calculate the forecast error covariance matrices and the gain matrix from a given geodynamo solution, which can then be used for sequential geomagnetic data assimilation. This component is very flexible and can be executed independently. It can also be easily integrated with arbitrary dynamo models. C1 [Sun, Zhibin] Colorado State Univ, USDA, UV B Monitoring & Res Program, Ft Collins, CO 80523 USA. [Kuang, Weijia] NASA, Goddard Space Flight Ctr, Planetary Geodynam Lab, Greenbelt, MD 20771 USA. RP Sun, ZB (reprint author), Colorado State Univ, USDA, UV B Monitoring & Res Program, Ft Collins, CO 80523 USA. EM sunzhib1@gmail.com RI Kuang, Weijia/K-5141-2012 OI Kuang, Weijia/0000-0001-7786-6425 FU NASA Earth Surface and Interior Program; NSF CSEDI program [EAR0757880]; NASA NAS FX We thank Dr. Andrew Tangborn for his suggestions and comments on assimilation algorithms, and Dr. Weiyuan Jiang for computing support. This work is supported by NASA Earth Surface and Interior Program, and by NSF CSEDI program under the grant EAR0757880. Part of the numerical simulation is supported by NASA NAS. NR 24 TC 0 Z9 0 U1 0 U2 2 PU CHINESE GEOSCIENCE UNION PI TAIPEI PA PO BOX 23-59, TAIPEI 10764, TAIWAN SN 1017-0839 EI 2311-7680 J9 TERR ATMOS OCEAN SCI JI Terr. Atmos. Ocean. Sci. PD FEB PY 2015 VL 26 IS 1 SI SI BP 53 EP 61 DI 10.3319/TAO.2014.08.19.05(GRT) PG 9 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Oceanography SC Geology; Meteorology & Atmospheric Sciences; Oceanography GA CC5HN UT WOS:000350389800006 ER PT J AU Hu, YB Bellaloui, N Tigabu, M Wang, JH Diao, J Wang, K Yang, R Sun, GY AF Hu, Yanbo Bellaloui, Nacer Tigabu, Mulualem Wang, Jinghong Diao, Jian Wang, Ke Yang, Rui Sun, Guangyu TI Gaseous NO2 effects on stomatal behavior, photosynthesis and respiration of hybrid poplar leaves SO ACTA PHYSIOLOGIAE PLANTARUM LA English DT Article DE Nitrogen dioxide; Photorespiration; Populus trees; Post-illumination CO2 burst; Stomatal behavior ID NITROGEN-DIOXIDE; CARBON-DIOXIDE; SO2; TREES; CO2; RESPONSES; ULTRASTRUCTURE; ASSIMILATION; DEPOSITION; EXPOSURE AB In this study, we used poplar as a model plant and investigated the effects of gaseous nitrogen dioxide (NO2, 4 mu l l(-1)) on stomatal conductance, photosynthesis, dark-and photorespiration of Populus alba 9 Populus berolinensis hybrid leaves using the photosynthesis system and scanning electron microscope technique. The results showed that net photosynthetic rates were significantly reduced in leaves exposed to 4 mu l l(-1) NO2 for 48 h as compared with leaves exposed to ambient carbon dioxide 380 mu l l(-1) and ambient NO2 <0.1 mu l l(-1) (the control) and the leaves exposed for 14 h. The decline of net photosynthetic rate was caused mainly by NO2 treatment. Dark respiration rates were dependent on co-action of the two factors (leaf temperature and NO2 treatment time). Postillumination carbon dioxide burst in the exposed leaves occurred at 13-15 s after turning the light off, whereas this phenomenon was absent in the control leaves. C1 [Hu, Yanbo; Diao, Jian; Wang, Ke; Yang, Rui; Sun, Guangyu] Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China. [Bellaloui, Nacer] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Tigabu, Mulualem] Swedish Univ Agr Sci, Southern Swedish Forest Res Ctr, SE-23050 Alnarp, Sweden. [Wang, Jinghong] Northeast Forestry Univ, Coll Landscape, Harbin 150040, Peoples R China. RP Hu, YB (reprint author), Northeast Forestry Univ, Coll Life Sci, 26 Hexing Rd,POB 123, Harbin 150040, Peoples R China. EM huybnefu@yahoo.com RI Hu, Yanbo/G-9095-2011 FU Fundamental Research Funds for the Central Universities [2572014CA24, DL10BB24]; National Natural Science Foundation [31300506]; Major project for the Heilongjiang Province Science and Technology Program [GZ13B004] FX This work was supported by grants from the "Fundamental Research Funds for the Central Universities" (Grant No. 2572014CA24; DL10BB24), 'National Natural Science Foundation' (Grant No. 31300506), and The Major project for the Heilongjiang Province Science and Technology Program (GZ13B004). The authors gratefully acknowledged Mr. Thomas D. Dahmer and Dr. Josirley de FC Carvalho for language correction. NR 44 TC 0 Z9 0 U1 2 U2 22 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0137-5881 EI 1861-1664 J9 ACTA PHYSIOL PLANT JI Acta Physiol. Plant. PD FEB PY 2015 VL 37 IS 2 AR UNSP 39 DI 10.1007/s11738-014-1749-8 PG 8 WC Plant Sciences SC Plant Sciences GA CC0TQ UT WOS:000350050100039 ER PT J AU Barnett, BA Holm, DG Koym, JW Wilson, RG Manter, DK AF Barnett, Brittany A. Holm, David G. Koym, Jeffery W. Wilson, Robert G. Manter, Daniel K. TI Site and Clone Effects on the Potato Root-Associated Core Microbiome and its Relationship to Tuber Yield and Nutrients SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Soil microbiome; Rhizosphere; Solanum tuberosum; 454 DNA pyrosequencing; Tuber nutrients; Bacteria ID GROWTH-PROMOTING RHIZOBACTERIA; 16S RIBOSOMAL-RNA; PLANT-GROWTH; SOIL TYPE; SP NOV.; DIVERSITY; CULTIVAR; DEVOSIA; COMMUNITIES; RHIZOSPHERE AB The aim of this study was to describe the variability in the root-associated bacterial community due to location and clone, and to determine whether an underlying core bacterial community exists that might benefit the quality of the potato crop. Root-associated bacterial communities from one growing season were examined with 454 sequencing. Variance analysis using perMANOVA attributed 45.4 % and 24.1 % of the community variability to site and clone effects, respectively. A total of 123 bacterial operational taxonomic units were correlated with tuber yield and/or tuber nutrient content, a majority belong to the order Rhizobiales. Rhizobiales bacteria are recognized contributors to crop nitrogen needs for many legumes; however, no known symbiotic relationship between potato roots and nitrogen fixing bacteria exists. Within the Rhizobiales order, the genus Devosia is a major contributor to both the presence/absence core "bacteriome" and the sparse partial least squares core "bacteriome," thus further exploration into this unknown relationship is warranted. C1 [Barnett, Brittany A.; Manter, Daniel K.] USDA ARS, Soil Plant Nutrient Res, Ft Collins, CO 80526 USA. [Holm, David G.] Colorado State Univ, San Luis Valley Res Ctr, Center, CO 81125 USA. [Koym, Jeffery W.] Texas A&M Univ, Agr Res & Extens Ctr, Lubbock, TX 79401 USA. [Wilson, Robert G.] Univ Calif, ANR Intermt Res & Extens Ctr, Tulelake, CA 96134 USA. RP Manter, DK (reprint author), USDA ARS, Soil Plant Nutrient Res, Ft Collins, CO 80526 USA. EM daniel.manter@ars.usda.gov NR 42 TC 2 Z9 2 U1 5 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2015 VL 92 IS 1 BP 1 EP 9 DI 10.1007/s12230-014-9405-9 PG 9 WC Agronomy SC Agriculture GA CC3GA UT WOS:000350233300001 ER PT J AU Rowley, JS Gray, SM Karasev, AV AF Rowley, Jenny S. Gray, Stewart M. Karasev, Alexander V. TI Screening Potato Cultivars for new Sources of Resistance to Potato virus Y SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato virus Y; Resistance; N genes ID TUBER NECROTIC RINGSPOT; NUCLEOTIDE-SEQUENCE; BIOLOGICAL CHARACTERIZATION; HYPERSENSITIVE RESISTANCE; MONOCLONAL-ANTIBODIES; TETRAPLOID POTATO; SOLANUM-TUBEROSUM; GENETIC DIVERSITY; GENOME SEQUENCE; STRAINS AB Potato virus Y (PVY) strains have been defined based on genetic reactions in potato indicators expressing hypersensitive reaction (HR) response due to the presence of three different N genes, and also based on genomic information. Nine strains are known currently, with five PVY strains defined biologically, PVYO, PVYC, PVYZ, PVYN, and PVYE. The genetic background of the majority of North American potato cultivars has so far been poorly characterized for the presence of N genes inducing HR towards different PVY strains. Here, the HR response was studied in eight potato cultivars, elicited by five strains of PVY circulating in North America. These PVY isolates included representative isolates of PVYN-Wi, PVYNA-N, PVYO, PVYZ, and PVYN strains. Potato cultivars tested included Russet Burbank, Russet Norkotah, Shepody, Ranger Russet, Western Russet, Alturas, Rio Grande Russet, and Yukon Gem, grown in the U.S., and standard indicators Desiree and Maris Bard with the known genetic background. Three additional strains, PVYN:O, PVY-NE11, and PVYE, were tested on Yukon Gem. Virus-free potato plants were mechanically inoculated with PVY inoculum, and local and systemic foliar symptoms were observed for 8 weeks post-inoculation under different climate-controlled conditions. Virus status of the inoculated plants was tested starting at 3 weeks post-inoculation, by serotype-specific ELISA and RT-PCR, in order to monitor successful infections and confirm the identity of the inoculated PVY isolate. This systematic approach allowed us to identify Ny (tbr) and Nz (tbr) genes present in several North American cultivars. Two more new, putative N genes were postulated to be expressed in the cultivar Yukon Gem, and one additional putative N gene was postulated to be expressed in two cultivars, Yukon Gem and Rio Grande Russet. These N genes may represent valuable sources of resistance against multiple strains of PVY. C1 [Rowley, Jenny S.; Karasev, Alexander V.] Univ Idaho, Dept PSES, Moscow, ID 83844 USA. [Gray, Stewart M.] Cornell Univ, USDA ARS, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. RP Karasev, AV (reprint author), Univ Idaho, Dept PSES, Moscow, ID 83844 USA. EM akarasev@uidaho.edu FU United States Department of Agriculture (USDA)-NIFA-NRI [2009-35600-05025]; USDA-NIFA-SCRI [2009-51181-05894]; USDA-ARS [58-5354-7-540, 58-5354-2-345, 58-1907-8-870]; Idaho State Department of Agriculture; Idaho Potato Commission; Washington State Potato Commission; Idaho Agricultural Experiment Station FX We are grateful to Lorie Ewing for help with maintenance of potato lines in tissue culture, and to Dr. Camille Kerlan for many helpful discussions. This work was funded in part through grants from the United States Department of Agriculture (USDA)-NIFA-NRI (2009-35600-05025), USDA-NIFA-SCRI (2009-51181-05894), USDA-ARS (58-5354-7-540, 58-5354-2-345, and 58-1907-8-870), Idaho State Department of Agriculture, Idaho Potato Commission, Washington State Potato Commission, and by the Idaho Agricultural Experiment Station. NR 49 TC 5 Z9 6 U1 1 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2015 VL 92 IS 1 BP 38 EP 48 DI 10.1007/s12230-014-9409-5 PG 11 WC Agronomy SC Agriculture GA CC3GA UT WOS:000350233300005 ER PT J AU Wang, Y Bethke, PC Drilias, MJ Schmitt, WG Bussan, AJ AF Wang, Yi Bethke, Paul C. Drilias, Michael J. Schmitt, William G. Bussan, Alvin J. TI A Multi-Year Survey of Stem-End Chip Defect in Chipping Potatoes (Solanum Tuberosum L.) SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE White potato; Stem-end chip defect; Chipping potato varieties; Potato chip quality; Average chip defect score; Severe chip defect incidence; Tuber sucrose and glucose concentration ID MAILLARD REACTION; COLOR DEVELOPMENT; REDUCING SUGARS; AMINO-ACIDS; ACRYLAMIDE; PRODUCTS; QUALITY AB One of the most serious tuber quality concerns of US chip potato growers is stem-end chip defect, which is defined as a localized post-fry discoloration in and adjacent to the vasculature on the stem end portion of potato chips. The severity and incidence of stem-end chip defect vary with growing location and variety, but quantitative data describing this are not available. A multi-year and location study was conducted to evaluate chipping potato varieties for tolerance to stem-end chip defect formation and to quantify defect severity and incidence regionally and temporally. It was observed that higher night temperature in July and August compared to the regional 30-year history was consistently associated with higher SECD severity. Average SECD severity was linearly correlated with incidence of chips having severe SECD. Chip color and tuber stem-end glucose content were significantly correlated with average SECD score across all treatments. Multiple tuber samplings from early growing season to mid post-harvest storage demonstrated that some varieties, such as Nicolet and Pike, had fewer stem-end chip defects than other varieties across years and locations. Stem-end chip defect was rarely observed prior to harvest, but was apparent at the time of harvest on multiple varieties depending on the year and location. Tuber stem-end sucrose and glucose prior to harvest were not indicators of defect development at harvest and out of storage, but pre-harvest chip color was significantly correlated with defect severity 10 weeks after preconditioning was finished. C1 [Wang, Yi; Bethke, Paul C.; Drilias, Michael J.; Schmitt, William G.; Bussan, Alvin J.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Bethke, Paul C.] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Wang, Y (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM wang52@wisc.edu FU United States Potato Board FX The authors thank Walter Farms, CSS Farms, Heartland Farms and their employees for helping us to conduct this study. The authors thank Frito-lay, Inc. for authorizing use of FL varieties. Special thanks are given to United States Potato Board Chip Committee for encouraging this research and to the United States Potato Board for funding this research. NR 34 TC 0 Z9 0 U1 1 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2015 VL 92 IS 1 BP 79 EP 90 DI 10.1007/s12230-014-9414-8 PG 12 WC Agronomy SC Agriculture GA CC3GA UT WOS:000350233300009 ER PT J AU Cooper, WR Alcala, PE Barcenas, NM AF Cooper, W. Rodney Alcala, Piedad E. Barcenas, Nina M. TI Relationship Between Plant Vascular Architecture and Within-Plant Distribution of 'Candidatus Liberibacter solanacearum' in Potato SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Liberibacter psyllaurous; Orthostichous; Bactericera cockerelli; Solanum ID PHLOEM-DEPENDENT MOVEMENT; ZEBRA CHIP DISEASE; REAL-TIME PCR; SYSTEMIC INDUCTION; TOMATO; PHYTOPLASMAS; PATHWAY; TUBERS; CITRUS; VIRUS AB 'Candidatus Liberibacter solanacearum' is an important pathogen of Solanaceous crops that causes zebra chip disease of potato. This pathogen is transmitted among plants by the potato psyllid Bactericera cockerelli. Within-plant spatial variability in Liberibacter infection impedes the ability to detect the bacterium before the onset of visible symptoms. The goal of our study was to test whether vascular architecture of potato explains the uneven distribution of Liberibacter after inoculation of leaves. The movement of rhodamine B among leaves was used to identify vascular connectivity among leaves. Three weeks after inoculating a single leaf with Liberibacter, the pathogen infected significantly more leaflets that had direct vascular connectivity with the inoculated leaf than leaflets with minimal connectivity. In a separate study, significantly more psyllids confined to whole leaves with direct vascular connectivity to a Liberibacter-infected leaf acquired the pathogen than did psyllids confined to leaves with indirect or partial connectivity to the infected leaf. Using fluorescence in situ hybridization, the pathogen was observed in the inner and outer phloem above and below the export leaf, respectively, corresponding with passive movement of Liberibacter in the phloem. Results of this study indicate that the distribution of Liberibacter in potato is at least partly limited by vascular architecture. This knowledge should improve the design of sampling methods to detect Liberibacter in asymptomatic plants. C1 [Cooper, W. Rodney] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Alcala, Piedad E.; Barcenas, Nina M.] Heritage Univ, Dept Nat Sci, Toppenish, WA 98948 USA. RP Cooper, WR (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Rodney.Cooper@ars.usda.gov RI Cooper, William/D-3205-2017 FU NSF-REU Award [1156603] FX Heather Headrick and Pauline Anderson provided technical assistance. Piedad Alcala was supported by NSF-REU Award # 1156603 to Heritage University (PI Nina Barcenas). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 26 TC 1 Z9 1 U1 2 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2015 VL 92 IS 1 BP 91 EP 99 DI 10.1007/s12230-014-9416-6 PG 9 WC Agronomy SC Agriculture GA CC3GA UT WOS:000350233300010 ER PT J AU Jansky, S Hamernik, A AF Jansky, Shelley Hamernik, Andy TI Rapid Cycling of Potato Tuber Generations by Overcoming Dormancy SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Gibberellic acid; Tuber dormancy AB Dormancy hinders progress in attempts to fast track potato tuber generations. In this study, we evaluated the ability of gibberellic acid (GA) to overcome dormancy in freshly harvested tubers of 11 potato cultivars in 2 years of field trials. Tubers were wounded and dipped in 0, 10, 100, and 1,000 ppm GA. Then they were planted in the field 5 days later. Vine length and stem number were measured throughout the season. Tubers were also harvested and weighed. Cultivars varied in their response to GA treatment. However, for all cultivars, wounding followed by treatment with 10 or 100 ppm effectively overcame dormancy. The 1,000 ppm treatment produced excessive vine growth and lower yield compared to the lower concentrations. Consequently, wounding of freshly harvested tubers, followed by a dip in 10 or 100 ppm GA is recommended to overcome tuber dormancy in programs interested in rapid cycling. C1 [Jansky, Shelley; Hamernik, Andy] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Jansky, Shelley] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. RP Jansky, S (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov NR 16 TC 0 Z9 1 U1 2 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD FEB PY 2015 VL 92 IS 1 BP 148 EP 152 DI 10.1007/s12230-014-9415-7 PG 5 WC Agronomy SC Agriculture GA CC3GA UT WOS:000350233300017 ER PT J AU Boardman, CR Glass, SV AF Boardman, C. R. Glass, Samuel V. TI Basement radon entry and stack driven moisture infiltration reduced by active soil depressurization SO BUILDING AND ENVIRONMENT LA English DT Article DE Radon; Moisture; Soil gas; Stack effect; Active soil depressurization; Permanent wood foundation ID INDOOR HUMIDITY; AIR; HOUSES AB This case study presents measurements of radon and moisture infiltration from soil gases into the basement of an unoccupied research house in Madison, Wisconsin, over two full years. The basement floor and exterior walls were constructed with preservative-treated lumber and plywood. In addition to continuous radon monitoring, measurements included building air tightness, indoor outdoor pressure difference, indoor and outdoor temperature and relative humidity, wood moisture content, and tracer gas decay. A single-zone air infiltration model was calibrated based on tracer gas measurements. Soil moisture infiltration was determined by mass conservation after accounting for all other moisture flows: humidification, air exchange, diffusion through the above-grade building envelope, sorption in hygroscopic materials, and moisture removal by air conditioning. A moisture balance methodology used in previous work was validated and improved here by including two time scales in the sorption modeling. Active soil depressurization was applied and shown to reduce both radon and soil moisture infiltration. The stack effect is shown to correlate well with soil moisture infiltration but does not fully explain radon entry. Published by Elsevier Ltd. C1 [Boardman, C. R.; Glass, Samuel V.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Boardman, CR (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM cboardman@fs.fed.us OI Boardman, Charles/0000-0002-9571-5601 FU Forest Products Laboratory of the U.S. Forest Service FX Research was funded by the Forest Products Laboratory of the U.S. Forest Service. This research was performed by U.S. government employees on official time and is therefore in the public domain and not subject to copyright. NR 43 TC 1 Z9 1 U1 3 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0360-1323 EI 1873-684X J9 BUILD ENVIRON JI Build. Environ. PD FEB PY 2015 VL 85 BP 220 EP 232 DI 10.1016/j.buildenv.2014.12.008 PG 13 WC Construction & Building Technology; Engineering, Environmental; Engineering, Civil SC Construction & Building Technology; Engineering GA CC2OQ UT WOS:000350184900024 ER PT J AU Tan, CX Schrader, KK Khan, IA Rimando, AM AF Tan, Cheng-Xia Schrader, Kevin K. Khan, Ikhlas A. Rimando, Agnes M. TI Activities of Wogonin Analogs and Other Flavones against Flavobacterium columnare SO CHEMISTRY & BIODIVERSITY LA English DT Article DE Antibacterial activity; Channel catfish; Columnaris disease; Flavobacterium columnare; Flavones; Wogonin ID PATHOGENS EDWARDSIELLA-ICTALURI; BARR-VIRUS ACTIVATION; CHANNEL CATFISH; K562/ADM CELLS; CANCER; DERIVATIVES; RESVERATROL; INHIBITION; OXIDATION; PUNCTATUS AB In our on-going pursuit to discover natural products and natural product-based compounds to control the bacterial species Flavobacterium columnare, which causes columnaris disease in channel catfish (Ictalurus punctatus), we synthesized flavone and chalcone analogs, and evaluated these compounds, along with flavonoids from natural sources, for their antibacterial activities against two isolates of F. columnare (ALM-00-173 and BioMed) using a rapid bioassay. The flavonoids chrysin (1a), 5,7-dihydroxy-4-methoxyflavone (11), isorhamnetin (26), luteolin (27), and biochanin A (29), and chalcone derivative 8b showed strong antibacterial activities against F. columnare ALM-00-173 based on minimum inhibition concentration (MIC) results. Flavonoids 1a, 8, 11, 13 (5,4-dihydroxy-7-methoxyflavone), 26, and 29 exhibited strong antibacterial activities against F. columnare BioMed based upon MIC results. The 24-h 50% inhibition concentration (IC50) results revealed that 27 and 29 were the most active compounds against F. columnare ALM-00-173 (IC50 of 7.5 and 8.5mg/l, resp.), while 26 and 29 were the most toxic compound against F. columnare BioMed (IC50 of 9.2 and 3.5mg/l, resp.). These IC50 results were lower than those obtained for wogonin against F. columnare ALM-00-173 and F. columnare BioMed (28.4 and 5.4mg/l, resp.). However, based on MIC results, none of the compounds evaluated in this study were as active as wogonin (MIC 0.3mg/l for each F. columnare isolate). Further modification of the wogonin structure to enhance antibacterial is of interest. C1 [Tan, Cheng-Xia] Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Zhejiang, Peoples R China. [Tan, Cheng-Xia; Schrader, Kevin K.; Rimando, Agnes M.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Khan, Ikhlas A.] Univ Mississippi, Thad Cochran Res Ctr, University, MS 38677 USA. RP Rimando, AM (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, POB 1848, University, MS 38677 USA. EM agnes.rimando@ars.usda.gov FU Chinese Scholarship Council FX We thank Marcuslene Harries and Phaedra Page for technical assistance. We are also grateful for the financial support to C.-X. T. from the Chinese Scholarship Council. NR 30 TC 0 Z9 0 U1 3 U2 10 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1612-1872 EI 1612-1880 J9 CHEM BIODIVERS JI Chem. Biodivers. PD FEB PY 2015 VL 12 IS 2 BP 259 EP 272 DI 10.1002/cbdv.201400181 PG 14 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA CC1PZ UT WOS:000350115000006 PM 25676507 ER PT J AU Li, Y Liu, SK Qin, ZK Waldbieser, G Wang, RJ Sun, LY Bao, LS Danzmann, RG Dunham, R Liu, ZJ AF Li, Yun Liu, Shikai Qin, Zhenkui Waldbieser, Geoff Wang, Ruijia Sun, Luyang Bao, Lisui Danzmann, Roy G. Dunham, Rex Liu, Zhanjiang TI Construction of a high-density, high-resolution genetic map and its integration with BAC-based physical map in channel catfish SO DNA RESEARCH LA English DT Article DE SNP; catfish; linkage map; physical map; genome ID SALMON SALMO-SALAR; SEX-SPECIFIC RECOMBINATION; TROUT ONCORHYNCHUS-MYKISS; COMPARATIVE GENOME ANALYSIS; MICROSATELLITE LINKAGE MAP; ATLANTIC SALMON; MEIOTIC RECOMBINATION; ICTALURUS-PUNCTATUS; SNP ARRAY; END SEQUENCES AB Construction of genetic linkage map is essential for genetic and genomic studies. Recent advances in sequencing and genotyping technologies made it possible to generate high-density and high-resolution genetic linkage maps, especially for the organisms lacking extensive genomic resources. In the present work, we constructed a high-density and high-resolution genetic map for channel catfish with three large resource families genotyped using the catfish 250K single-nucleotide polymorphism (SNP) array. A total of 54,342 SNPs were placed on the linkage map, which to our knowledge had the highest marker density among aquaculture species. The estimated genetic size was 3,505.4 cM with a resolution of 0.22 cM for sex-averaged genetic map. The sex-specific linkage maps spanned a total of 4,495.1 cM in females and 2,593.7 cM in males, presenting a ratio of 1.7 : 1 between female and male in recombination fraction. After integration with the previously established physical map, over 87% of physical map contigs were anchored to the linkage groups that covered a physical length of 867 Mb, accounting for similar to 90% of the catfish genome. The integrated map provides a valuable tool for validating and improving the catfish whole-genome assembly and facilitates fine-scale QTL mapping and positional cloning of genes responsible for economically important traits. C1 [Li, Yun; Liu, Shikai; Qin, Zhenkui; Wang, Ruijia; Sun, Luyang; Bao, Lisui; Dunham, Rex; Liu, Zhanjiang] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA. [Li, Yun; Liu, Shikai; Qin, Zhenkui; Wang, Ruijia; Sun, Luyang; Bao, Lisui; Dunham, Rex; Liu, Zhanjiang] Auburn Univ, Aquat Genom Unit, Program Cell & Mol Biosci, Auburn, AL 36849 USA. [Waldbieser, Geoff] USDA ARS, Warmwater Aquaculture Res Unit, Stoneville, MS 38776 USA. [Danzmann, Roy G.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. RP Liu, ZJ (reprint author), Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA. EM liuzhan@auburn.edu FU Agriculture and Food Research Initiative Competitive Grant from the US Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) [2012-67015-19410]; USDA Aquaculture Research Program Competitive Grant from USDA NIFA [2014-70007-22395]; USDA Aquaculture Research Program Competitive Grant [2014-70007-22395] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant (No. 2012-67015-19410) from the US Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) and partially supported by USDA Aquaculture Research Program Competitive Grant (No. 2014-70007-22395) from USDA NIFA. Funding to pay the Open Access publication charges for this article was provided by a grant from USDA Aquaculture Research Program Competitive Grant no. 2014-70007-22395. NR 64 TC 11 Z9 11 U1 2 U2 20 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1340-2838 EI 1756-1663 J9 DNA RES JI DNA Res. PD FEB PY 2015 VL 22 IS 1 BP 39 EP 52 DI 10.1093/dnares/dsu038 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA CC5YU UT WOS:000350442100005 PM 25428894 ER PT J AU Prendeville, HR Steven, JC Galloway, LF AF Prendeville, Holly R. Steven, Janet C. Galloway, Laura F. TI Spatiotemporal variation in deer browse and tolerance in a woodland herb SO ECOLOGY LA English DT Article DE Campanula; Campanulastrum americanum; fecundity; herbivory; intraspecific variation; range-wide; tolerance; white-tailed deer ID WHITE-TAILED DEER; PLANT TOLERANCE; FLOWERING PHENOLOGY; IPOMOPSIS-AGGREGATA; NATURAL-SELECTION; HERBIVORY; EVOLUTION; DAMAGE; COMMUNITIES; ECOSYSTEMS AB Herbivory can shape the dynamics of plant populations, including effects on survival and reproduction, and is in turn affected by environmental factors that vary in space and time. White-tailed deer are significant herbivores in North America that have been broadly documented to affect plant reproductive success. If variation in the frequency and impact of herbivory by deer correlates with a broad-scale latitudinal gradient, climactic effects may be important for shaping plant-herbivore interactions. Alternatively, a lack of broad-scale gradients would suggest local factors such as plant community composition and deer densities are affecting herbivory. To investigate broad-scale patterns of deer herbivory, we examined the frequency and reproductive consequences of deer browse over three years in 17 populations of Campanulastrum americanum spanning the latitudinal extent of its range. Even though deer are overabundant throughout the range of C. americanum, we found spatiotemporal variation in deer browse frequency (0-0.96, mean 0.46) and its effects on plant reproductive success. The four southernmost populations experienced high levels of herbivory, and were responsible for generating a negative relationship between latitude and herbivory. In general, patterns of variation in the frequency and impact of herbivory across the entire latitudinal gradient pointed to the importance of local rather than broad-scale factors. Within a population, deer consumed larger plants. Across many populations and years, average fitnesses of browsed and uneaten plants were similar, suggesting that plants are tolerant to browse. However, since large plants have greater reproductive success and are more likely to be browsed, tolerance may be influenced by plant size. When plant size was accounted for, most populations did not fully compensate for browsing. There was no relationship between browsing intensity and tolerance, suggesting that browsing may be too variable to consistently select for tolerance, or that increases in deer density are too recent for increased tolerance to evolve. C1 [Prendeville, Holly R.; Galloway, Laura F.] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA. [Steven, Janet C.] Sweet Briar Coll, Dept Biol, Sweet Briar, VA 24595 USA. RP Prendeville, HR (reprint author), US Forest Serv, Pacific NW Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM hp8j@virginia.edu FU NSF [DEB-1020717]; Sweet Briar College; ROA supplement FX Funding for this work by NSF DEB-1020717 and an ROA supplement; J. C. Steven was supported by a faculty fellowship from Sweet Briar College. We are thankful for assistance in locating and providing access to wild populations by A. Stiles, W. Bailey, Florida Caverns and Torreya State Parks of Florida, M. Alford, S. Threlkeld, C. Potts, Land Trust of North Alabama, R. McCoy, Tennessee State Parks, B. R. Keener, J. Bender, Kentucky State Nature Preserves Commission, B. James, Floracliffs State Nature Preserve, M. Crews, Lincoln State Park of Indiana, M. Becus, Ault Park, C. Bladow, The Nature Conservancy, S. Ross, B. Yankowiak, Little River Wetlands Project, Fernwood Botanical Garden and Nature Preserve, T. Basset, M. Richards, and Fort Custer Training Center. We are grateful for field assistance from Carolyn Beans, Anna Greenlee, Karen Barnard-Kubow, and Rowan Sprague. NR 45 TC 1 Z9 1 U1 4 U2 21 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD FEB PY 2015 VL 96 IS 2 BP 471 EP 478 DI 10.1890/14-0653.1 PG 8 WC Ecology SC Environmental Sciences & Ecology GA CC6OI UT WOS:000350484600018 PM 26240868 ER PT J AU Limbu, S Keena, MA Long, D Ostiguy, N Hoover, K AF Limbu, Samita Keena, Melody A. Long, David Ostiguy, Nancy Hoover, Kelli TI Scymnus camptodromus (Coleoptera: Coccinellidae) Larval Development and Predation of Hemlock Woolly Adelgid (Hemiptera: Adelgidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Scymnus camptodromus; Adelges tsugae; development time; prey consumption; degree-day ID TSUGAE HOMOPTERA; TEMPERATURE; CANADENSIS AB Development time and prey consumption of Scymnus (Neopullus) camptodromus Yu and Liu (Coleoptera: Coccinellidae) larvae by instar, strain, and temperature were evaluated. S. camptodromus, a specialist predator of hemlock woolly adelgid Adelges tsugae (Annand) (Hemiptera: Adelgidae), was brought to the United States from China as a potential biological control agent for A. tsugae. This beetle has been approved for removal from quarantine but has not yet been field released. We observed that temperature had significant effects on the predator's life history. The larvae tended to develop faster and consume more eggs of A. tsugae per day as rearing temperature increased. Mean egg consumption per day of A. tsugae was less at 15 degrees C than at 20 degrees C. However, as larvae took longer to develop at the lower temperature, the total number of eggs consumed per instar during larval development did not differ significantly between the two temperatures. The lower temperature threshold for predator larval development was estimated to be 5 degrees C, which closely matches the developmental threshold of A. tsugae progrediens. Accumulated degree-days for 50% of the predator neonates to reach adulthood was estimated to be 424. Although temperature had a significant effect on larval development and predation, it did not impact survival, size, or sex ratio of the predator at 15 and 20 degrees C. Furthermore, no remarkable distinctions were observed among different geographical populations of the predator. C1 [Limbu, Samita; Long, David; Ostiguy, Nancy; Hoover, Kelli] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Limbu, Samita; Long, David; Ostiguy, Nancy; Hoover, Kelli] Penn State Univ, Ctr Chem Ecol, University Pk, PA 16802 USA. [Keena, Melody A.] US Forest Serv, No Res Stn, Hamden, CT 06514 USA. RP Hoover, K (reprint author), Penn State Univ, Dept Entomol, 501 ASI Bldg, University Pk, PA 16802 USA. EM kxh25@psu.edu FU USDA Forest Service Northeastern Area (NA) State and Private Forestry (S&PF) Technology Development part of the Joint Hemlock Woolly Adelgid Initiative [13-CA-11422224-060]; Pennsylvania Department of Conservation and Natural Resources to Improve Management of HemlockWoolly Adelgid FX We thank Paul Moore, Vicente Sanchez, and Alice Vandel for providing technical assistance; Wenhua Lu, Li Li, Jianhua Zhou, and Gouyue Yu for obtaining the adult lady beetles to start the colonies; Michael Montgomery for his review of an earlier version of the paper; and John Stanovick for statistical analyses. This work was funded in part by two USDA Forest Service Northeastern Area (NA) State and Private Forestry (S&PF) Technology Development competitive grants to K.H.: 13-CA-11422224-060 part of the Joint Hemlock Woolly Adelgid Initiative funding and 09-DG-1142004-330 with allocation through Pennsylvania Department of Conservation and Natural Resources to Improve Management of HemlockWoolly Adelgid. NR 22 TC 1 Z9 1 U1 2 U2 6 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD FEB PY 2015 VL 44 IS 1 BP 81 EP 89 DI 10.1093/ee/nvu006 PG 9 WC Entomology SC Entomology GA CC5ZO UT WOS:000350444300010 PM 26308809 ER PT J AU Wagschal, K Jordan, DB Lee, CC Younger, A Braker, JD Chan, VJ AF Wagschal, Kurt Jordan, Douglas B. Lee, Charles C. Younger, Aunna Braker, Jay D. Chan, Victor J. TI Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans SO ENZYME AND MICROBIAL TECHNOLOGY LA English DT Article DE Uronate dehydrogenase; Thermal stability; Steady-state ordered binding pattern; Glucuronic acid; Galacturonic acid; Glucaric acid; Galactaric acid ID NITRIC-ACID OXIDATION; D-GALACTURONIC ACID; GLUCURONIC-ACID; CRYSTAL-STRUCTURE; PURIFICATION; SALEXIGENS; SYRINGAE; PECTINS AB Enzyme catalysts will be vital in the development of synthetic biology approaches for converting pectinic monosaccharides from citrus and beet processing waste streams to value-added materials. We describe here the biophysical and mechanistic characterization of uronate dehydrogenases from a wide variety of bacterial sources that convert galacturonic acid, the predominate building block of pectin from these plant sources, and glucuronic acid to their corresponding dicarboxylic acids galactarate and glucarate, the latter being a DOE top value biochemical from biomass. The enzymes from Pseudomonas syringae and Polaromonas naphthalenivorans were found to have the highest reported k(cat)(glucuronic acid) values, on the order of 220-270 s(-1). The thermal stability of this enzyme type is described for the first time here, where it was found that the K-t((0.5)) value range was >20 degrees C, and the enzyme from Chromohalobacter was moderately thermostable with K-t((0.5)) = 62.2 degrees C. The binding mechanism for these bi-substrate enzymes was also investigated in initial rate experiments, where a predominately steady-state ordered binding pattern was indicated. Published by Elsevier Inc. C1 [Wagschal, Kurt; Lee, Charles C.; Younger, Aunna; Chan, Victor J.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Jordan, Douglas B.; Braker, Jay D.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Wagschal, K (reprint author), USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. EM kurt.wagschal@ars.usda.gov FU United States Department of Agriculture CRIS [5325-41000-049-00, 3620-41000-118-00D]; National Institute of Food and Agriculture grant [2012-03998] FX This work was supported by United States Department of Agriculture CRIS 5325-41000-049-00 and National Institute of Food and Agriculture grant 2012-03998 (A.Y., V.C., C.C.L., K.W.), and United States Department of Agriculture CRIS 3620-41000-118-00D (D.B.J. and J.D.B.). The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. USDA is an equal opportunity provider and employer. NR 30 TC 5 Z9 5 U1 1 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0141-0229 EI 1879-0909 J9 ENZYME MICROB TECH JI Enzyme Microb. Technol. PD FEB PY 2015 VL 69 BP 62 EP 68 DI 10.1016/j.enzmictec.2014.12.008 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CC1DE UT WOS:000350080600009 PM 25640726 ER PT J AU Czarnitzki, D Grimpe, C Toole, AA AF Czarnitzki, Dirk Grimpe, Christoph Toole, Andrew A. TI Delay and secrecy: does industry sponsorship jeopardize disclosure of academic research? SO INDUSTRIAL AND CORPORATE CHANGE LA English DT Article ID UNIVERSITY-RESEARCH; LIFE SCIENCES; FACULTY; INSTITUTIONS; PERFORMANCE; ECONOMICS; QUALITY AB The viability of modern open science norms and practices depend on public disclosure of new knowledge, methods, and materials. Aggregate data from the OECD show a broad shift in the institutional financing structure that supports academic research from public to private sponsorship. This article examines the relationship between industry sponsorship and restrictions on disclosure using individual-level data on German academic researchers. Accounting for self-selection into extramural sponsorship, our evidence strongly supports the perspective that industry sponsorship jeopardizes public disclosure of academic research. C1 [Czarnitzki, Dirk] Katholieke Univ Leuven, Dept Managerial Econ Strategy & Innovat, B-3000 Leuven, Belgium. [Czarnitzki, Dirk] Katholieke Univ Leuven, Ctr R&D Monitoring ECOOM, Leuven, Belgium. [Czarnitzki, Dirk; Toole, Andrew A.] Ctr European Econ Res ZEW, Mannheim, Germany. [Grimpe, Christoph] Copenhagen Business Sch, Dept Innovat & Org Econ, DK-2000 Frederiksberg, Denmark. [Grimpe, Christoph] Lund Univ, Res Policy Inst, Lund, Sweden. [Toole, Andrew A.] USDA, Econ Res Serv, Washington, DC 20036 USA. RP Toole, AA (reprint author), Ctr European Econ Res ZEW, Mannheim, Germany. EM dirk.czarnitzki@econ.kuleuven.be; christoph@cbs.dk; atoole@ers.usda.gov FU Flemish Science Foundation (FWO) [G.0501.11] FX Czarnitzki gratefully acknowledges funding from the Flemish Science Foundation (FWO grant: G.0501.11). The views expressed in this article are the authors' and do not necessarily represent the views of the US Department of Agriculture or the Economic Research Service. NR 41 TC 3 Z9 3 U1 3 U2 10 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0960-6491 EI 1464-3650 J9 IND CORP CHANGE JI Ind. Corp. Change PD FEB PY 2015 VL 24 IS 1 BP 251 EP 279 DI 10.1093/icc/dtu011 PG 29 WC Business; Economics; Management SC Business & Economics GA CC2WL UT WOS:000350205500008 ER PT J AU Slaton, MR AF Slaton, Michele R. TI The roles of disturbance, topography and climate in determining the leading and rear edges of population range limits SO JOURNAL OF BIOGEOGRAPHY LA English DT Article DE California; climate; distribution modelling; disturbance; leading edge; range limit; rear edge; succession; topography ID PLANT MIGRATION RATES; BACKGROUND EXTINCTION; SPECIES DISTRIBUTIONS; DISTRIBUTION MODELS; GLOBAL CHANGE; QUATERNARY; ECOLOGY; REFUGIA; SHIFTS; CONSERVATION AB Aim To identify the ecosystem features most important for differentiating leading edges from rear edges of plant population distributions, and for distinguishing both from contiguous-range sites. Location Eastern California, USA. Methods Habitats supporting multiple plant populations at their range edges were analysed, including 32 rear-edge, 32 leading-edge and 526 control sites within contiguous ranges. Multivariate modelling techniques, including general linear and additive modelling, ordination and classification trees were used to identify the biotic and abiotic factors that control range edges. Results Rear edges were associated with lower solar radiation and potential evapotranspiration, as would generally be expected as ranges shift in a warming climate regime. Leading edges were associated with lower topographical positions, such as canyon bottoms, and were characterized by recent disturbance and early successional stages. Rear edges occurred mainly in undisturbed sites. Main conclusions The interacting roles of biological and physical factors in determining population range limits have recently gained attention as the need to predict ecosystem shifts in a changing climate increases. Further understanding and recognition of disturbance in facilitating the expansion of native species' ranges and in conserving relict populations will enhance the ability of land managers to maintain biodiversity and ecosystem resilience as climate continues to change. C1 US Forest Serv, USDA, Inyo Natl Forest, Bishop, CA 93514 USA. RP Slaton, MR (reprint author), US Forest Serv, USDA, Inyo Natl Forest, 351 Pacu Lane,Suite 200, Bishop, CA 93514 USA. EM mslaton02@fs.fed.us FU Inyo National Forest; Death Valley National Park FX I thank the Inyo National Forest and Death Valley National Park for partial funding, and both those agencies and the Bureau of Land Management Bishop Office for permits. I thank Paul Slaton, Sue Farley, Del Wiens, Kathleen Nelson, Sue Weis and Hugh Safford for countless field adventures and useful discussions. I am indebted to Jessica Kindred, Rachel Fixsen, Danny Margoles and others for field assistance, and to Wally Woolfenden, Steve McLaughlin, Peter Linder and two anonymous referees for comments on previous versions of this manuscript. NR 68 TC 5 Z9 5 U1 3 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0305-0270 EI 1365-2699 J9 J BIOGEOGR JI J. Biogeogr. PD FEB PY 2015 VL 42 IS 2 BP 255 EP 266 DI 10.1111/jbi.12406 PG 12 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA CC7OX UT WOS:000350558900006 ER PT J AU Kim, JY Young, JA Gunther, NW Lee, JL AF Kim, Ji Yeun Young, Janay A. Gunther, Nereus W. Lee, Jung-Lim TI INHIBITION OF SALMONELLA BY BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA DERIVED FROM US KIMCHI AND BROILER CHICKEN SO JOURNAL OF FOOD SAFETY LA English DT Article ID LACTOBACILLUS-PLANTARUM STRAIN; ACTIVE SURVEILLANCE NETWORK; IN-VITRO; BILE TOLERANCE; HUMAN FECES; SP NOV.; PROBIOTICS; FOOD; IDENTIFICATION; ENTERITIDIS AB In this study, lactic acid bacteria (LAB) were isolated from kimchi prepared in the U.S.A. and from broiler chickens. The resulting isolates were examined for potential probiotic characteristics. A total of 488 LAB strains were isolated, and among them, 16 originating from kimchi and 14 from broilers produced bacteriocin-like substances, which showed antimicrobial effects against SalmonellaEnteritidis, Heidelberg, Newport and Typhimurium. These strains were investigated with regard to additional probiotic properties such as tolerance to gastric juice and bile, resistance to enzymes, and antibiotics susceptibilities. Lactobacillus casei or paracasei Cab-18, Lb.saniviri Cuc-1, and Leuconostoc mesenteroides Com-54 isolated from kimchi and Lb.johnsoniiF-6 and Lb.crispatusF-59 isolated from broiler chicken demonstrated promising probiotic properties with the ability to produce bacteriocin-like substances with relatively strong antimicrobial activity against Salmonella. These strains could potentially be applied as supplements to poultry in order to reduce Salmonella contamination. Practical ApplicationsSalmonellosis associated with consumption of contaminated meats, especially poultry products, is a major concern due to the common outbreaks in the U.S.A. Although it has been shown that lactic acid bacteria (LAB) could reduce Salmonella contaminations, LAB isolated from kimchi have not been studied for the potential to inhibit the persistence of Salmonella spp. in live poultry. The present study screened LAB, which showed antimicrobial effects against four clinically important serotypes of Salmonella. Additionally, we selected LAB that had additional probiotic properties, which would allow for the strains use as potential feed additives. Therefore, the LAB strains isolated in this study could be applied as feed supplements to poultry for the purpose of reducing colonization of Salmonella in poultry as well as contamination during processing. C1 [Kim, Ji Yeun; Young, Janay A.; Lee, Jung-Lim] Delaware State Univ, Coll Agr & Related Sci, Dept Human Ecol, Food Sci Program, Dover, DE 19901 USA. [Gunther, Nereus W.] ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Wyndmoor, PA USA. RP Lee, JL (reprint author), Delaware State Univ, Coll Agr & Related Sci, Dept Human Ecol, Food Sci Program, Dover, DE 19901 USA. EM jlee@desu.edu FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2012-69003-19628] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant (No. 2012-69003-19628) from the USDA National Institute of Food and Agriculture. The authors would like to thank Dr. Jeanine Harter-Dennis, and the poultry research center at University of Maryland Eastern Shore, for providing chicken materials. NR 56 TC 2 Z9 3 U1 5 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0149-6085 EI 1745-4565 J9 J FOOD SAFETY JI J. Food Saf. PD FEB PY 2015 VL 35 IS 1 BP 1 EP 12 DI 10.1111/jfs.12141 PG 12 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CC3PL UT WOS:000350261300001 ER PT J AU Fushimi, S Miyazawa, T Kimura, F Nakagawa, K Burdeos, GC Miyazawa, T AF Fushimi, Saki Miyazawa, Taiki Kimura, Fumiko Nakagawa, Kiyotaka Burdeos, Gregor Carpentero Miyazawa, Teruo TI Young Persimmon Ingestion Suppresses Lipid Oxidation in Rats SO JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY LA English DT Article DE persimmon; ascorbic acid; tannin; antioxidant; phospholipid hydroperoxide ID LOW-DENSITY-LIPOPROTEIN; PHOSPHATIDYLCHOLINE HYDROPEROXIDE; PHOSPHOLIPID HYDROPEROXIDES; DIOSPYROS-KAKI; ANTIOXIDANT; PLASMA; SUPPLEMENTATION; PREVENT; CELLS; ASSAY AB Persimmon is widely eaten in Asia and the nutritional components of young and mature persimmons differ. Although raw young persimmon has a strong bitter taste and is difficult to eat, the beneficial health effects of young persimmon powder have attracted attention in recent years. Young persimmon has been suggested to have hypolipidemic activity as well as other biological effects. However, there has been little investigation of the beneficial effects of young persimmon. In the present study, we investigated the antioxidative effects of persimmon in an animal study and compared the effects of young persimmon and mature persimmon. Six-week-old male F344 rats were divided into three groups and fed a standard diet, young persimmon diet, or mature persimmon diet for 4 wk. The young persimmon and mature persimmon groups were fed a diet containing 5% (w/w) freeze-dried young or mature persimmon. We analyzed phosphatidylcholine hydroperoxide (PCOOH) levels in the rats as a biomarker of membrane lipid peroxidation. Our study showed that plasma PCOOH levels were significantly lower in the young persimmon group (36.1 +/- 28.5 pmol/ mL plasma) than in the control group (120 +/- 66 pmol/mL plasma). No significant difference was observed between the mature persimmon group (57.3 +/- 15.6 pmol/mL plasma) and the control group. It is possible that ascorbic acid and soluble tannin contribute to the difference in the antioxidant effects of young and mature persimmons. These results indicated that intake of young persimmon contributes to the reduction of plasma phospholipid hydroperoxide levels in rats. C1 [Fushimi, Saki; Miyazawa, Taiki; Kimura, Fumiko; Nakagawa, Kiyotaka; Burdeos, Gregor Carpentero; Miyazawa, Teruo] Tohoku Univ, Food & Biodynam Chem Lab, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan. [Fushimi, Saki] Tohoku Univ, Sch Med, Sendai, Miyagi 9808575, Japan. [Miyazawa, Taiki] Tufts Univ, Vasc Biol Lab, Jean Mayer USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Miyazawa, Teruo] Tohoku Univ, Food & Biotechnol Innovat Project, NICHe, Sendai, Miyagi 9808579, Japan. RP Miyazawa, T (reprint author), Tohoku Univ, Food & Biodynam Chem Lab, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan. EM miyazawa@m.tohoku.ac.jp RI Miyazawa, Taiki/G-5994-2014 FU Japanese Society for the Promotion of Science (JSPS, Tokyo,Japan) [20228002]; JSPS FX The present study was supported in part by a Grantin-Aid for Scientific Research (Kakenhi Kiban(s) 20228002) from the Japanese Society for the Promotion of Science (JSPS, Tokyo, Japan). Taiki Miyazawa was supported by a JSPS Postdoctoral Fellowship for Research Abroad (http://www.jsps.go.jp/). NR 29 TC 1 Z9 1 U1 0 U2 3 PU CENTER ACADEMIC PUBL JAPAN PI TOKYO PA 2-4-16 YAYOI, BUNKYO-KU, TOKYO, 113-0032, JAPAN SN 0301-4800 EI 1881-7742 J9 J NUTR SCI VITAMINOL JI J. Nutr. Sci. Vitaminol. PD FEB PY 2015 VL 61 IS 1 BP 90 EP 95 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CC7ID UT WOS:000350540000013 PM 25994144 ER PT J AU Krug, PW Holinka, LG O'Donnell, V Reese, B Sanford, B Fernandez-Sainz, I Gladue, DP Arzt, J Rodriguez, L Risatti, GR Borca, MV AF Krug, Peter W. Holinka, Lauren G. O'Donnell, Vivian Reese, Bo Sanford, Brenton Fernandez-Sainz, Ignacio Gladue, Douglas P. Arzt, Jonathan Rodriguez, Luis Risatti, Guillermo R. Borca, Manuel V. TI The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome SO JOURNAL OF VIROLOGY LA English DT Article ID MULTIGENE FAMILY-360; GENE; REPLICATION; DNA; DETERMINANT; MACROPHAGES; INFECTION; SEQUENCE; GROWTH; REGION AB African swine fever virus (ASFV) causes a contagious and often lethal disease of feral and domestic swine. Experimental vaccines derived from naturally occurring, genetically modified, or cell culture-adapted ASFV have been evaluated, but no commercial vaccine is available to control African swine fever (ASF). We report here the genotypic and phenotypic analysis of viruses obtained at different passages during the process of adaptation of a virulent ASFV field isolate from the Republic of Georgia (ASFV-G) to grow in cultured cell lines. ASFV-G was successively passaged 110 times in Vero cells. Viruses obtained at passages 30, 60, 80, and 110 were evaluated in vitro for the ability to replicate in Vero cells and primary swine macrophages cultures and in vivo for assessing virulence in swine. Replication of ASFV-G in Vero cells increased with successive passages, corresponding to a decreased replication in primary swine macrophages cultures. In vivo, progressive loss of virus virulence was observed with increased passages in Vero cells, and complete attenuation of ASFV-G was observed at passage 110. Infection of swine with the fully attenuated virus did not confer protection against challenge with virulent parental ASFV-G. Full-length sequence analysis of each of these viruses revealed significant deletions that gradually accumulated in specific areas at the right and left variable ends of the genome. Mutations that result in amino acid substitutions and frameshift mutations were also observed, though in a rather limited number of genes. The potential importance of these genetic changes in virus adaptation/attenuation is discussed. IMPORTANCE The main problem in controlling ASF is the lack of vaccines. Attempts to produce vaccines by adaptation of ASFV to cultured cell lines have been made. These attempts led to the production of attenuated viruses that conferred only homologous protection. Specifics regarding adaptation of these isolates to cell cultures have been insufficiently described. Details like the numbers of passages required to obtain attenuated viruses, genetic modifications introduced into the virus genomes along passages, and the extent of attenuation and induced protective efficacy are not readily available. In this study, we assessed the changes that lead to decreased growth in swine macrophages and to attenuation in swine. Loss of virulence, probably associated with limited replication in vivo, may lead to the lack of protective immunity in swine observed after challenge. This report provides valuable information that can be used to further the understanding of ASFV gene function, virus attenuation, and protection against infection. C1 [Krug, Peter W.; Holinka, Lauren G.; O'Donnell, Vivian; Sanford, Brenton; Fernandez-Sainz, Ignacio; Gladue, Douglas P.; Arzt, Jonathan; Rodriguez, Luis; Borca, Manuel V.] ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [O'Donnell, Vivian; Fernandez-Sainz, Ignacio; Gladue, Douglas P.; Risatti, Guillermo R.] Univ Connecticut, Dept Pathobiol & Vet Sci, CANHR, Storrs, CT USA. [Reese, Bo] Univ Connecticut, Ctr Appl Genet & Technol, Storrs, CT USA. RP Borca, MV (reprint author), ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM manuel.borca@ars.usda.gov RI Fernandez Sainz, Ignacio/I-3046-2016; OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178; Arzt, Jonathan/0000-0002-7517-7893 FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-11-X-00077, HSHQPM-12-X-00005]; ARS/USDA-University of Connecticut [SCA 58-1940-1-190] FX This project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under award numbers HSHQDC-11-X-00077 and HSHQPM-12-X-00005. We thank ARS/USDA-University of Connecticut SCA 58-1940-1-190 for partially supporting this work. NR 26 TC 11 Z9 11 U1 0 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD FEB PY 2015 VL 89 IS 4 BP 2324 EP 2332 DI 10.1128/JVI.03250-14 PG 9 WC Virology SC Virology GA AZ9UT UT WOS:000348562800034 PM 25505073 ER PT J AU Grienenberger, E Fletcher, JC AF Grienenberger, Etienne Fletcher, Jennifer C. TI Polypeptide signaling molecules in plant development SO CURRENT OPINION IN PLANT BIOLOGY LA English DT Review ID FLORAL ORGAN ABSCISSION; LATERAL ROOT EMERGENCE; LIGAND-RECEPTOR PAIRS; STEM-CELL FATE; ARABIDOPSIS-THALIANA; SECRETED PEPTIDE; INFLORESCENCE-DEFICIENT; CRISPR/CAS SYSTEM; SHOOT DEVELOPMENT; AUXIN-TRANSPORT AB Intercellular communication mediated by small signaling molecules is a key mechanism for coordinating plant growth and development. In the past few years, polypeptide signals have been shown to play prominent roles in processes as diverse as shoot and root meristem maintenance, vascular differentiation, lateral root emergence, and seed formation. Signaling components such as CLV1 and the IDA-HAE/HSL2 signaling module have been discovered to regulate distinct developmental processes in different tissues. Recent studies have also uncovered novel polypeptide-receptor interactions, intracellular components and downstream target genes, adding complexity to our picture of polypeptide signaling networks. Finally, new families of plant polypeptides, such as the GLV/RGF/CLEL and ESF factors, are being identified, the functions of which we are only beginning to understand. C1 [Grienenberger, Etienne; Fletcher, Jennifer C.] Univ Calif Berkeley, USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Grienenberger, Etienne; Fletcher, Jennifer C.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. RP Fletcher, JC (reprint author), Univ Calif Berkeley, USDA ARS, Ctr Plant Gene Express, 800 Buchanan St, Albany, CA 94710 USA. EM jfletcher@berkeley.edu OI Grienenberger, Etienne/0000-0002-0555-3208 FU US Department of Agriculture [CRIS 5335-21000-038-00D]; US National Science Foundation [IOS 1257429] FX We are grateful to Peter DiGennaro for helpful comments. Funding for our research is provided by the US Department of Agriculture (CRIS 5335-21000-038-00D) and the US National Science Foundation (IOS 1257429). NR 61 TC 13 Z9 13 U1 9 U2 48 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1369-5266 EI 1879-0356 J9 CURR OPIN PLANT BIOL JI Curr. Opin. Plant Biol. PD FEB PY 2015 VL 23 BP 8 EP 14 DI 10.1016/j.pbi.2014.09.013 PG 7 WC Plant Sciences SC Plant Sciences GA CB8LK UT WOS:000349880900003 PM 25449721 ER PT J AU Fraedrich, SW Harrington, TC Best, GS AF Fraedrich, S. W. Harrington, T. C. Best, G. S. TI Xyleborus glabratus attacks and systemic colonization by Raffaelea lauricola associated with dieback of Cinnamomum camphora in the southeastern United States SO FOREST PATHOLOGY LA English DT Article ID REDBAY AMBROSIA BEETLE; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; LAUREL WILT; NONCONDUCTIVE SAPWOOD; PHOEBE OIL; 1ST REPORT; PATHOGEN; TREES; QUERCIVORA; LAURACEAE AB Laurel wilt, caused by Raffaelea lauricola, is responsible for extensive mortality of redbay and other American members of the Lauraceae in the southeastern United States. Raffaelea lauricola is a mycangial symbiont of the redbay ambrosia beetle (Xyleborus glabratus), and the beetle and fungus were accidentally introduced from Asia. Branch dieback of camphortree (Cinnamomum camphora), an Asian member of the Lauraceae, has been occasionally observed in areas where laurel wilt has decimated redbay populations, and R.lauricola was isolated from such camphortrees. However, the role of X.glabratus and R.lauricola in this branch dieback remains unclear. Examination of camphortrees on Jekyll Island, Georgia showed that healthy-appearing trees and those with branch dieback had been attacked by X.glabratus, but the trees with branch dieback had four times as many beetle attacks. Raffaelea lauricola was routinely isolated from discoloured xylem near beetle tunnels in healthy trees and those with dieback. Single-point inoculations with R.lauricola on stems of mature, healthy camphortree trees failed to induce wilt-like symptoms or branch dieback, although areas of discoloration were scattered throughout the xylem, and R.lauricola was reisolated irregularly at various heights in some inoculated trees. In growth chamber experiments, single-point inoculations with R.lauricola resulted in systemic colonization but no wilt symptoms or branch dieback in camphortree saplings. In contrast, inoculations at multiple points along the stem (simulating multiple attacks by the vector) caused branch dieback and wilt-like symptoms, including a brownish, diffuse discoloration of the xylem. Camphortree appears to be more resistant than American species of Lauraceae to the vascular wilt caused by R.lauricola. The fungus does colonize camphortrees systemically, however, and can apparently cause branch dieback. This suggests that the fungus may provide brood material for X.glabratus in Asia as it does in the southeastern United States. C1 [Fraedrich, S. W.; Best, G. S.] ARS, Southern Res Stn, USDA, Athens, GA 30602 USA. [Harrington, T. C.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA USA. RP Fraedrich, SW (reprint author), ARS, Southern Res Stn, USDA, Athens, GA 30602 USA. EM sfraedrich@fs.fed.us NR 41 TC 4 Z9 4 U1 1 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 EI 1439-0329 J9 FOREST PATHOL JI Forest Pathol. PD FEB PY 2015 VL 45 IS 1 BP 60 EP 70 DI 10.1111/efp.12124 PG 11 WC Forestry SC Forestry GA CB8YX UT WOS:000349919500009 ER PT J AU Brown, CR AF Brown, Charles R. TI Russet Burbank: No Ordinary Potato SO HORTSCIENCE LA English DT Article AB The 'Russet Burbank' potato cultivar currently occupies first place in acreage planted in North America and is worth in the United States $1.4 billion annually. It is a sport of 'Burbank's Seedling', which was selected by Luther Burbank in 1873. The ancestry of Burbank stems from a plant introduction brought to the United States by the Rev. Chauncey Goodrich of New York State in 1853. The priorities of potato breeding had been transformed by repetitive crop failures caused by the emergence of the plant pathogen Phytophthora infestans. Modern testing suggests that derivatives of Goodrich's potatoes were slightly more resistant to Phytophthora. Burbank discovered a single fruit on one of these derivatives, 'Early Rose', in his mother's garden. Taking the 23 true seeds, he nursed them to full-sized plants and selected ultimately No. 15. It produced an unusually high yield of large, very oblong tubers, stored well, and was a good eating potato. Burbank's life was destined for a long career in California and he attempted to sell the clone to J.H.J. Gregory of Gregory's Honest Seeds, a successful businessman. Ultimately Gregory agreed to buy it for $150, far less than Burbank wanted, but enough to propel him to California. Gregory named the potato 'Burbank's Seedling', which no doubt engendered fame for the entrepreneur. Luther Burbank had been allowed by Gregory to keep 10 tubers, which became the seed source for the 'Burbank's Seedling' to spread north and south along the West Coast of North America with a crop value, stated by Burbank, of $14 million in 1914. It is not clear that Luther Burbank prospered from 'Burbank's Seedling' in the West. A skin sport with a russet skin was found in Colorado in 1902 and was advertised by a seed company under the name 'Netted Gem'. 'Burbank's Seedling' per se disappeared from commerce and 'Netted Gem' slowly increased, finding a special niche in production of French fry potatoes. It is clear that Luther Burbank gained tremendous insight into the dynamics of hybridization in revealing genetic variation from clonally propagated species. During the rest of his career he would use this technique to produce new and amazing forms of numerous food and ornamental species. 'Burbank's Seedling' was his entrez into the world of plant breeding. C1 USDA ARS, WSU IAREC Hort LA, Prosser, WA 99350 USA. RP Brown, CR (reprint author), USDA ARS, WSU IAREC Hort LA, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM chuck.brown@ars.usda.gov NR 23 TC 0 Z9 0 U1 3 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 157 EP 160 PG 4 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900003 ER PT J AU Anderson, NO Olsen, RT AF Anderson, Neil O. Olsen, Richard T. TI A Vast Array of Beauty: The Accomplishments of the Father of American Ornamental Breeding, Luther Burbank SO HORTSCIENCE LA English DT Article ID LILY NELLIE-WHITE; CACTACEAE; OPUNTIA; CROP AB Luther Burbank (1849-1926) was a prolific ornamental plant breeder, who worked with 91 genera of ornamentals, from Abutilon to Zinnia, and released nearly 1000 cultivars to the industry. His innovative work included both herbaceous and woody plant materials as well as ornamental vegetables such as corn, tomatoes, and spineless cacti. His most popular ornamental release, the shasta daisy hybrids first released in 1901, is still on the global market. This article focuses on Luther Burbank's breeding techniques with ornamental plants and how both the germplasms that he developed and his methodologies used permeate modern flower breeding. Genera with the highest number of cultivars bred and released by Burbank include Amaryllis, Hippeastrum, and Crinum followed by Lilium, Hemerocallis, Watsonia, Papaver, Gladiolus, Dahlia, and Rosa. With Lilium, he pioneered breeding the North American native lily species, particularly those from the Pacific coastal region, producing the eponymous Lilium x barbankii. Burbank's breeding enterprise was designed to be self-sustaining based on profits from selling the entire product line of a new cultivar or crop only to wholesale firms, who then held exclusives for propagation and selling, although financial hardships necessitated selling retail occasionally. Entire lots of selected seedlings were sold to the highest bidder with Burbank setting the price in his annual catalogs such as the Burbank Hybrid Lilies lot for U.S. $250,000 or some of the "very handsome, hardy ones" for U.S. $250 to U.S. $10,000 each. Other flower cultivars also commanded high prices such as seedling Giant Amaryllis that sold for U.S. $1.55/bulb in 1909. Cacti were another area of emphasis (he released more than 63 cultivars) from the spineless fruiting and forage types (Opuntia ficus-indica, O. tuna, O. vulgaris) to flowering ornamentals such as O. basilaris, Cereus chilensis, and Echinopsis mulleri. Interest in cacti during 1909-15 rivaled the Dutch Tulip mania with exorbitant fees for a single "slab" of a cultivar, speculative investments, controversy with noted cacti specialists (particularly David Griffiths), and lawsuits by The Burbank Company. Although most cultivars have been lost, Burbank's reputation as the Father of American Ornamental Breeding remains admirable from critics and devotees alike. C1 [Anderson, Neil O.] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Olsen, Richard T.] ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Anderson, NO (reprint author), Univ Minnesota, Dept Hort Sci, 1970 Folwell Ave, St Paul, MN 55108 USA. EM ander044@umn.edu FU ASHS History of Horticultural Science Working Group; Minnesota Agricultural Experiment Station FX This paper was part of the workshop "Contributions of Luther Burbank: Plant Breeding Artist and Legend" held 22 July 2013 at the ASHS Conference, Palm Desert, CA, and sponsored by the ASHS History of Horticultural Science Working Group.; This research has been supported in whole or in part by the Minnesota Agricultural Experiment Station. NR 63 TC 0 Z9 0 U1 2 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 161 EP 188 PG 28 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900004 ER PT J AU Callahan, A Dardick, C Tosetti, R Lalli, D Scorza, R AF Callahan, Ann Dardick, Chris Tosetti, Roberta Lalli, Donna Scorza, Ralph TI 21st Century Approach to Improving Burbank's 'Stoneless' Plum SO HORTSCIENCE LA English DT Article ID ARABIDOPSIS AB The theme running through many of Luther Burbank's breeding programs was to make plants more tailored to human uses. Mr. Burbank thought that the stone in plum fruits was unessential to a tree that was propagated vegetatively, so he chose stoneless plums as a breeding goal. He made two releases, 'Miracle' in 1903 and his final and almost perfect 'Conquest' in 1916, which he considered one of his best accomplishments in plum breeding. 'Conquest' had only a grain of stone and flavor and size comparable to the best French types of the time but was not commercially successful. In view of the current desire for convenience food such as seedless fruit (citrus, grapes, watermelon) and advanced knowledge of genetics and breeding technologies, we have taken up where Mr. Burbank left off in the production of a better than "almost perfect" stoneless plum. We began by locating what were most likely remnants from Mr. Burbank's breeding program and we are now using 21st century technology to achieve a completely stoneless, high-quality plum fruit. These technologies include molecular markers, genetic engineering, and accelerated breeding cycles (FasTrack). Initial experiments had characterized the stoneless trait as a decrease in the number of endocarp cells that form the stone. We defined the time critical to the formation of endocarp by analyzing gene expression of a number of transcription factors involved with determining endocarp cells. We identified genes that were expressed differently during this period between normal stone cultivars and one of the stoneless cultivars. In addition, we targeted genes for genetic engineering to reduce the lignification in endocarp and to reduce or convert endocarp cells to non-lignifying cells. A system, FasTrack, using a flowering gene from poplar, has been incorporated to reduce the juvenility period and eliminate the seasonal aspect of fruiting to see the results of the breeding as well as the genetic engineering approach much faster. The combination of these approaches is now in place to attempt to improve on Mr. Burbank's stoneless plum. C1 [Callahan, Ann; Dardick, Chris; Tosetti, Roberta; Lalli, Donna; Scorza, Ralph] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Callahan, A (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM ann.callahan@ars.usda.gov FU ASHS History of Horticultural Science Working Group FX This paper was part of the workshop "Contributions of Luther Burbank: Plant Breeding Artist and Legend" held 22 July 2013 at the ASHS Conference, Palm Desert, CA, and sponsored by the ASHS History of Horticultural Science Working Group. NR 17 TC 4 Z9 4 U1 2 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 195 EP 200 PG 6 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900006 ER PT J AU Preece, JE McGranahan, G AF Preece, John E. McGranahan, Gale TI Luther Burbank's Contributions to Walnuts SO HORTSCIENCE LA English DT Article AB Luther Burbank began making controlled crosses between walnut species in the late 19th century after hearing about a "supposed natural European hybrid walnut." He crossed Juglans hindsii (northern California black walnut) x J. regia (Persian walnut) and produced progeny that he named 'Paradox' because of its extremely fast growth and other "anomalies." He also crossed two American species, J. hindsii x J. nigra (eastern black walnut), producing 'Royal' walnut progeny that were fast-growing and prolific nut producers. A third interspecific hybrid was a cross between J.. ailantifolia (Japanese walnut) x regia that resulted in extremely vigorous progeny but was not named. He observed segregation in the F-2 populations and described giants and dwarfs as reversions to ancestral forms. Luther Burbank also made selections for walnut scion cultivars and was especially interested in thin-shelled nuts. He collected seeds from a J. regia growing in San Francisco because it produced regularly and had very high-quality nuts with relatively thin but poorly sealed shells. He selected one of its seedlings as 'Santa Rosa Soft-Shell' and described it as bearing large crops of nuts that were nearly white with thin shells and delicious white meat. Burbank's contributions to the walnut industry endure to this day, especially through the widespread use of seedling and clonal 'Paradox' walnut rootstocks. C1 [Preece, John E.] Univ Calif Davis, Natl Clonal Germplasm Repository, USDA ARS, Davis, CA 95616 USA. [McGranahan, Gale] Univ Calif Davis, Walnut Improvement Program, Davis, CA 95616 USA. RP Preece, JE (reprint author), Univ Calif Davis, Natl Clonal Germplasm Repository, USDA ARS, One Shields Ave, Davis, CA 95616 USA. EM John.Preece@ars.usda.gov FU ASHS History of Horticultural Science Working Group FX This paper was part of the workshop "Contributions of Luther Burbank: Plant Breeding Artist and Legend" held 22 July 2013 at the ASHS Conference, Palm Desert, CA, and sponsored by the ASHS History of Horticultural Science Working Group. NR 7 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 201 EP 204 PG 4 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900007 ER PT J AU Hummer, KE Finn, CE Dossett, M AF Hummer, Kim E. Finn, Chad E. Dossett, Michael TI Luther Burbank's Best Berries SO HORTSCIENCE LA English DT Article ID RUBUS AB Luther Burbank, the quintessential nurseryman of the early 20th century, remarked that small fruit was the "Cinderella of the pomological family." He stated that although tree fruits had been improved to the point of an almost uncountable number of cultivars, it was the time and responsibility of his generation and those to follow to develop the small fruit for human consumption. Burbank had a penchant for detecting potential qualities of unusual plants and his broad association with plant explorers at the U.S. Department of Agriculture and elsewhere allowed him to examine diverse wild berry species. He obtained seeds of many small fruit species from throughout the world. He made wide crosses within and between these genera and species. Burbank selected and named many cultivars to be introduced through his nursery and elsewhere. He named and released 40 blackberries, raspberries (Rubies L.), and strawberries (Fragaria L.); four grapes (Vitis L.); and a hybrid Solanum that he named 'Sunberry'. He sometimes exaggerated their descriptions for promotion or public recognition. For example, Rubus x loganobaccus 'Phenomenal' was, he stated, "far superior in size, quality, color, and productivity..." to 'Loganberry'. Unfortunately, this cultivar was not a commercial success. Burbank made a few crosses and sold what he considered to be improved species, e.g., 'Himalaya Giant' blackberry R. armeniacus). He created new common names for foreign species, e.g., balloon berry (R. illecebrosus) and Mayberry (R. palmatus), to better market them. However, his amazingly keen observations of thornlessness, pigment diversity, and recognition of repeat flowering and fruiting in blackberries, raspberries, and strawberries, were insightful of the needs of future industry. Burbank was a disciple of Darwin and his theory of natural selection. Burbank's classic breeding approach, to make wide crosses, produce large numbers of hybrid seedlings, choose significant seedlings with his traits of choice, and backcross to the desired parent for several generations, was successful, although he did not know of ploidy or gene recombination. Unfortunately, the 'Himalaya blackberry', now ubiquitous in hedgerows and fields throughout the Pacific Northwest in the United States, is designated as a federal noxious weed. Although not presently in commercial production, three of his Rubies cultivars ('Burbank Thornless', 'Snowbank', and 'Phenomenal') are preserved in the U.S. Department of Agriculture, National Clonal Germplasm Repository, in Corvallis, OR. C1 [Hummer, Kim E.] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97331 USA. [Finn, Chad E.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Dossett, Michael] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. RP Hummer, KE (reprint author), ARS, USDA, Natl Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR 97331 USA. EM Kim.Hummer@ars.usda.gov FU ASHS History of Horticultural Science Working Group FX This paper was part of the workshop "Contributions of Luther Burbank: Plant Breeding Artist and Legend" held 22 July 2013 at the ASHS Conference, Palm Desert, CA, and sponsored by the ASHS History of Horticultural Science Working Group. NR 12 TC 0 Z9 0 U1 3 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 205 EP 210 PG 6 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900008 ER PT J AU Fernandez-Salvador, J Strik, BC Bryla, DR AF Fernandez-Salvador, Javier Strik, Bernadine C. Bryla, David R. TI Liquid Corn and Fish Fertilizers Are Good Options for Fertigation in Blackberry Cultivars Grown in an Organic Production System SO HORTSCIENCE LA English DT Article ID MARION TRAILING BLACKBERRY; WEED MANAGEMENT-PRACTICES; THORNLESS BLACKBERRY; NITROGEN-FERTILIZATION; YIELD COMPONENTS; CROPPING SYSTEMS; PLANT-GROWTH; IRRIGATION; NITRATE; VEGETABLES AB The impact of organic fertilizer source on the growth, fruit quality, and yield of blackberry (Rubus L. subgenus Rubus Watson) cultivars (Marion and Black Diamond) grown in a machine-harvested, organic production system for the processed market was evaluated from 2011 to 2013. The planting was established in Spring 2010 using approved practices for organic production and was certified in 2012. Plants were irrigated using a dripline under a woven polyethylene groundcover (weed mat) installed for weed management. Two sources of liquid fertilizer were evaluated: 1) a corn steep liquor and fish waste digestion blend ("corn"; 2.5N-1.1P-1.2K); and 2) a fish solubles and molasses blend ("fish"; 4N-0P-1.7K). Fertilizers were applied by fertigation through the drip system at rates of 56 kg.ha(-1) nitrogen (N) per year in 2011-12 and 90 kg.ha(-1) N in 2013. The impact of fertigation on drip system performance was evaluated with two maintenance options, "flushing" and "no flushing" of the driplines. Total yield differed among years, whereas fruit soluble solids concentration and firmness as well as floricane biomass at pruning showed a year x cultivar interaction. 'Black Diamond' had greater total yield and average fruit weight than 'Marion', but produced a greater proportion unmarketable fruit. There was no effect of fertilizer source on yield, fruit quality, primocane length, or primocanes/plant in any year with the exception of fruit weight, which was greater with corn than with fish. 'Marion' had a greater floricane biomass when fertilized with fish than with corn. Soil nutrients were within the recommended range, except for boron (B), which was below recommended levels. Only soil nitrate-N was affected by fertilizer source, which was greater in 'Marion' than in 'Black Diamond' when fertilized with fish. Primocane leaf tissue nutrient concentrations were within recommended levels for all nutrients, except for calcium (Ca) and B, which were below recommended standards in both cultivars. Primocane leaf potassium (K) and zinc (Zn) concentrations were greater with fish than with corn. There was no fertilizer source or maintenance effect on emitter flow rate of the drip system in either year. However, flow rates decreased an average of 4.5% in the first year and 19% in the second year. Overall, there were no differences between the fertilizers on plant growth, yield, or fruit quality, and both fertilizers were suitable for planting establishment. C1 [Fernandez-Salvador, Javier; Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Bryla, David R.] ARS, USDA, Hort Crop Res Unit, Corvallis, OR 97330 USA. RP Strik, BC (reprint author), Oregon State Univ, Dept Hort, 4017 ALS, Corvallis, OR 97331 USA. EM bernadine.strik@oregonstate.edu FU Northwest Center for Small Fruits Research; USDA National Institute of Food and Agriculture [OREI 2010-01940, ORE00409] FX We appreciate research funding support provided by the Northwest Center for Small Fruits Research, the USDA National Institute of Food and Agriculture (Formula Grant no. OREI 2010-01940; ORE00409), our industry contributors and collaborators, and the assistance provided by Gil Buller, Amanda Vance, and Emily Vollmer. NR 57 TC 12 Z9 12 U1 4 U2 19 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 225 EP 233 PG 9 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900011 ER PT J AU Fernandez-Salvador, J Strik, BC Zhao, YY Finn, CE AF Fernandez-Salvador, Javier Strik, Bernadine C. Zhao, Yanyun Finn, Chad E. TI Trailing Blackberry Genotypes Differ in Yield and Postharvest Fruit Quality during Establishment in an Organic Production System SO HORTSCIENCE LA English DT Article ID WEED MANAGEMENT-PRACTICES; COMPONENTS; NAVAJO AB Four blackberry (Rubus L. subgenus Rubus Watson) cultivars ('Obsidian', 'Black Diamond', `Metolius', 'Onyx') and two advanced selections (ORUS 1939-4 and ORUS 2635-1) were evaluated during the establishment years of an organic production system for fresh market. The planting was established in Spring 2010 using approved practices for organic production and was certified organic in 2012, the first fruiting year. Plants were irrigated using a dripline under a woven polyethylene groundcover (weed mat) installed for weed management. Liquid fertilizers injected through the drip system were used at rates of 56 kg.ha(-1) total nitrogen (N) in 2011-12 and 90 kg.ha(-1) total N in 2013. Genotypes differed in the level of nutrients measured in primocane leaves. Tissue phosphorus (P), potassium (K), sulfur (S), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn) concentrations were within the recommended standards, but tissue calcium (Ca), magnesium (Mg), and boron (B) were deficient in some or all genotypes. Although two cultivars and both advanced selections responded well in terms of plant growth and yield to the organic production system used, yields in 'Onyx' and `Metolius' were considered low for commercial production. In contrast, the higher yielding 'Obsidian' and ORUS-2635-1 appeared to be the best suited for organic fresh market production as a result of larger fruit size, greater fruit firmness, higher sugar-to-acid ratios, lower postharvest percent moisture loss in ORUS-2635-1, and the longest number of marketable storage days at 5 degrees C in 'Obsidian'. C1 [Fernandez-Salvador, Javier; Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Zhao, Yanyun] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA. [Finn, Chad E.] ARS, USDA, Hort Crop Res Unit, Corvallis, OR 97330 USA. RP Strik, BC (reprint author), Oregon State Univ, Dept Hort, 4017 ALS, Corvallis, OR 97331 USA. EM bernadine.strik@oregonstate.edu FU Northwest Center for Small Fruits Research; USDA National Institute of Food and Agriculture [OREI 2010-01940, ORE00409] FX We appreciate research funding support provided by the Northwest Center for Small Fruits Research, the USDA National Institute of Food and Agriculture (Formula Grant no. OREI 2010-01940; ORE00409), our industry contributors and collaborators, and the assistance provided by Gil Buller, Emily Vollmer, and Amanda Vance. NR 37 TC 9 Z9 9 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 240 EP 246 PG 7 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900013 ER PT J AU Cho, AH Chase, CA Treadwell, DD Koenig, RL Morris, JB Morales-Payan, JP AF Cho, Alyssa H. Chase, Carlene A. Treadwell, Danielle D. Koenig, Rosalie L. Morris, John Bradley Morales-Payan, Jose Pablo TI Apical Dominance and Planting Density Effects on Weed Suppression by Sunn Hemp (Crotalaria juncea L.) SO HORTSCIENCE LA English DT Article ID LEGUME COVER CROP; SOIL AB A field study was conducted in 2008 and 2009 in Citra, FL, to evaluate the effects of seeding rate and removal of apical dominance of sunn hemp (Crotalaria juncea L.) on weed suppression and seed production by sunn hemp. Three seeding rates of sunn hemp were used: a representative seed production rate of 11 kg.ha(-1), an intermediate seeding rate of 28 kg.ha(-1), and a cover crop seeding rate of 45 kg.ha(-1). Cutting the main stem at 3, 4, or 5 weeks after planting to break apical dominance was compared with an uncut treatment. Cutting had no significant effect on shoot biomass, photosynthetically active radiation (PAR) penetrating the canopy, and nondestructive leaf area index (LAI). As a result, cutting also had no effect on weed density and biomass in 2008 and very little effect in 2009. Increase in seeding rate resulted in linear decrease in PAR and increase in LAI in both years. Seeding rate had a greater effect on suppression of weed biomass than on suppression of weed density. There was a linear decline in sunn hemp branching with increased seeding rate in 2009 and, averaged across years, flower number decreased linearly with increased seeding rate. Cutting to break apical dominance induced branching but had no effect on flower number. No seed pod production occurred and we postulate that the lack of seed production may be the result of the absence of effective pollinators in fall when short-day varieties of sunn hemp flower in Florida. C1 [Cho, Alyssa H.; Chase, Carlene A.; Treadwell, Danielle D.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Koenig, Rosalie L.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Morris, John Bradley] ARS, USDA, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Morales-Payan, Jose Pablo] Univ Puerto Rico, Mayaguez, PR 00681 USA. RP Chase, CA (reprint author), Univ Florida, Dept Hort Sci, POB 110690, Gainesville, FL 32611 USA. EM cachase@ufl.edu FU Southern Region Sustainable Agriculture Research and Education [LS08-205] FX This study was partially funded by the Southern Region Sustainable Agriculture Research and Education grant LS08-205. NR 20 TC 3 Z9 3 U1 2 U2 13 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 263 EP 267 PG 5 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900017 ER PT J AU Guo, YH Olsen, RT Kramer, M Pooler, M AF Guo, Yonghong Olsen, Richard T. Kramer, Matthew Pooler, Margaret TI Effective Bioassays for Evaluating Boxwood Blight Susceptibility Using Detached Stem Inoculations SO HORTSCIENCE LA English DT Article ID CYLINDROCLADIUM-PSEUDONAVICULATUM; 1ST REPORT; BUXICOLA AB Two simple and rapid in vitro bioassays using detached stems were developed for evaluating the susceptibility of boxwood genotypes to the blight disease caused by Calonectria pseudonaviculata (Crous et al.) L. Lombard et al. Individual leaves were inoculated on detached stems or entire detached stems were sprayed to assess susceptibility. Both assay systems were optimized for inoculum concentration and disease rating time. The assay methods described here require minimal plant material and inoculum, especially the leaf inoculation assay, which uses as few as six leaves per stem and 500 spores per leaf for inoculation. The stem spray inoculation produced less variable results and was easier for quantifying susceptibility but required more inoculum than the leaf inoculation assay. No differences between the assays were found for the cultivars tested. The leaf inoculation assay is best used when limited plant material or inoculum is available; the spray inoculation of detached stems is suitable when larger plants are available. C1 [Guo, Yonghong; Olsen, Richard T.; Pooler, Margaret] ARS, USDA, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD 20705 USA. [Kramer, Matthew] ARS, USDA, Biometr Consulting Serv, Beltsville, MD 20705 USA. RP Pooler, M (reprint author), ARS, USDA, US Natl Arboretum Floral & Nursery Plants Res Uni, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Margaret.Pooler@ars.usda.gov FU Floral and Nursery Research Initiative FX Research presented in this article was funded in part through the Floral and Nursery Research Initiative. NR 17 TC 1 Z9 1 U1 2 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 268 EP 271 PG 4 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900018 ER PT J AU Reed, SM Alexander, LW AF Reed, Sandra M. Alexander, Lisa W. TI 'Queen of Hearts' Oak leaf Hydrangea SO HORTSCIENCE LA English DT Article C1 [Reed, Sandra M.; Alexander, Lisa W.] ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit,Otis L Floyd Nur, Mcminnville, TN 37110 USA. RP Alexander, LW (reprint author), ARS, USDA, US Natl Arboretum, Floral & Nursery Plants Res Unit,Otis L Floyd Nur, 472 Cadillac Lane, Mcminnville, TN 37110 USA. EM Lisa.Alexander@ars.usda.gov NR 8 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD FEB PY 2015 VL 50 IS 2 BP 310 EP 311 PG 2 WC Horticulture SC Agriculture GA CB9ED UT WOS:000349933900024 ER PT J AU Jennings, V Gaither, CJ AF Jennings, Viniece Gaither, Cassandra Johnson TI Approaching Environmental Health Disparities and Green Spaces: An Ecosystem Services Perspective SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH LA English DT Article ID PHYSICAL-ACTIVITY; PUBLIC-HEALTH; BUILT ENVIRONMENT; UNITED-STATES; CLIMATE-CHANGE; URBAN FORESTS; NATURAL-ENVIRONMENT; NEIGHBORHOOD GREEN; POLITICAL ECOLOGY; MENTAL-HEALTH AB Health disparities occur when adverse health conditions are unequal across populations due in part to gaps in wealth. These disparities continue to plague global health. Decades of research suggests that the natural environment can play a key role in sustaining the health of the public. However, the influence of the natural environment on health disparities is not well-articulated. Green spaces provide ecosystem services that are vital to public health. This paper discusses the link between green spaces and some of the nation's leading health issues such as obesity, cardiovascular health, heat-related illness, and psychological health. These associations are discussed in terms of key demographic variables-race, ethnicity, and income. The authors also identify research gaps and recommendations for future research. C1 [Jennings, Viniece; Gaither, Cassandra Johnson] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. RP Jennings, V (reprint author), US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. EM vjennings02@fs.fed.us; cjohnson09@fs.fed.us NR 128 TC 11 Z9 11 U1 10 U2 79 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1660-4601 J9 INT J ENV RES PUB HE JI Int. J. Environ. Res. Public Health PD FEB PY 2015 VL 12 IS 2 BP 1952 EP 1968 DI 10.3390/ijerph120201952 PG 17 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA CC2XZ UT WOS:000350209800050 PM 25674782 ER PT J AU Pascacio-Villafan, C Williams, T Sivinski, J Birke, A Aluja, M AF Pascacio-Villafan, C. Williams, T. Sivinski, J. Birke, A. Aluja, M. TI Costly Nutritious Diets do not Necessarily Translate into Better Performance of Artificially Reared Fruit Flies (Diptera: Tephritidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE artificial diet; nutrient content; diet cost; mass rearing; Anastrepha ludens ID FLY DIPTERA; BIOLOGICAL-CONTROL; BACTROCERA-TRYONI; ANASTREPHA-LUDENS; LARVAL DIET; COLONIZATION; PARASITOIDS; PARAMETERS; QUALITY; MANGO AB Protein, lipid, carbohydrate, and energy contents of three artificial diets (Xal2, Met1, and Met2) used for laboratory-rearing and mass-rearing the Mexican fruit fly, Anastrepha ludens (Loew), for a sterile insect technique program were measured. The larval survival, pupation, pupal weight, adult emergence, sex ratio, and flight capacity of the flies reared on each of these diets were also quantified. The diet with the highest nutrient and energy content was Xal2 followed by Met2 and Met1, but larval recovery and percent pupation was significantly higher in flies reared on either the Met1 or Met2 diets. A. ludens reared on Xal2 exhibited the highest proportion of adults capable of flight. No other response variable differed significantly among the three diets tested. This suggests that a high content of nutrients and multiple sources of protein (dried yeast and wheat germ in the case of the Xal2 diet) do not necessarily improve overall performance or fly quality. We conclude that nutritious diets for A. ludens can be modified to reduce their cost without compromising the performance of artificially reared flies. C1 [Pascacio-Villafan, C.; Williams, T.; Birke, A.; Aluja, M.] Inst Ecol AC INECOL, Xalapa 91070, Veracruz, Mexico. [Sivinski, J.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Pascacio-Villafan, C (reprint author), Inst Ecol AC INECOL, Carretera Antigua Coatepec 351, Xalapa 91070, Veracruz, Mexico. EM cpascacio@hotmail.com RI Williams, Trevor/F-1447-2011 OI Williams, Trevor/0000-0003-3414-3440 FU Asociacion de Productores, Empacadores y Exportadores de Aguacate de Michoacan, A.C. (APEAM); Campana Nacional contra Moscas de la Fruta SAGARPA-IICA-INECOL; CONACYT scholarship FX We thank Dulce Diaz Tolentino and Adriana Santos Ramiro for technical assistance and the Programa Moscafrut in Metapa de Dominguez, Chiapas, Mexico, and Pablo Montoya for providing diet ingredients. Rodrigo Lasa (INECOL) facilitated flight testing equipment. Juan Rull and Larissa Guillen (INECOL) critically reviewed an earlier draft. This study was funded by the Asociacion de Productores, Empacadores y Exportadores de Aguacate de Michoacan, A.C. (APEAM), and the Campana Nacional contra Moscas de la Fruta SAGARPA-IICA-INECOL. This work is part of the doctoral thesis of C.P.V. C.P.V. acknowledges receipt of a CONACYT scholarship. NR 42 TC 2 Z9 2 U1 0 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 53 EP 59 DI 10.1093/jee/tou033 PG 7 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700008 PM 26470103 ER PT J AU Yokoyama, VY Cambron, SE Muhareb, J AF Yokoyama, Victoria Y. Cambron, Sue E. Muhareb, Jeannette TI Hessian Fly (Diptera: Cecidomyiidae) Mortality in Export Bale Compressors and Response to a Hydrogen Phosphide and Carbon Dioxide Gas Mixture SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Mayetiola destructor (Say); hydrogen phosphide and carbon dioxide gas; commodity treatment; quarantine security ID MULTIPLE QUARANTINE TREATMENT; LEAF BEETLE COLEOPTERA; WRAPPED BALES; LARGE-SIZE; HAY; FUMIGATION; JAPAN; CHRYSOMELIDAE; TEMPERATURE; EFFICACY AB Hessian fly, Mayetiola destructor (Say), puparial mortality was evaluated in three modern hay compressors that produce compressed standard and large-size bales for export to Asia-Pacific countries. Pressure on bales ranged from 93.4 to 139.4 kg/cm(2), causing 90.0-99.9% mortality of 10,891-23,164 puparia. Puparial response to a cylinderized hydrogen phosphide (1.8-2%) and carbon dioxide (97.8-98%) gas mixture was evaluated as a potential quarantine treatment using 2-4 d-exposures to low, medium, and high doses of 0.73-0.86, 1.05-1.26, and 1.39-1.56 mg/liter, and temperatures of 5.87 +/- 1.14, 9.84 +/- 0.05, 16.14 +/- 0.14, and 20.35 +/- 0.11 degrees C. Accumulative concentration multiplied by time products (mg h/liter) at all fumigation temperatures for low, medium, and high fumigant doses were 34.9-37.7, 52.2-54.3, and 67.9-73.1 for 2 d; 52.7-60.6, 77.9-89.2, and 102.1-110.7 for 3 d; and 69.9-82.0, 99.4-118.2, and 132.3-146.8 for 4 d, respectively. An increase in mortality was significantly related to an increase in fumigation duration at 5, 10, and 15 degrees C, and an increase in fumigant dose at 10 and 15 degrees C. Puparial mortality ranged from 97.2 to 100% at all doses and durations at 20 degrees C with no survivors at the highest dose for 3 d and the mid- and highest dose for 4 d. Bale compression is currently used in the first phase of a multiple quarantine treatment to control potential Hessian fly contaminants in exported hay. The novel fumigant may have application as a single quarantine treatment for noncompressed, standard exported bales. C1 [Yokoyama, Victoria Y.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Cambron, Sue E.] USDA ARS, Crop Prod & Pest Control Res, W Lafayette, IN 47907 USA. [Muhareb, Jeannette] Dried Fruit Assoc Calif, Fresno, CA 93721 USA. RP Yokoyama, VY (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM victoria.yokoyama.science@gmail.com FU USDA-ARS, Parlier, CA; University of California Davis; Anderson Hay & Grain, Ellensburg, WA; USDA-APHIS-PPQ, Ellensburg, WA; National Hay Association FX We are grateful to Sean Pelham and Brandon A. Simons, USDA-ARS, Parlier, CA; Hana A. Yokoyama-Hatch, University of California Davis; Ron Anderson, Anderson Hay & Grain, Ellensburg, WA; Rollie J. Bernth, Marla J. Bernth, and Joseph J. Wahle, Ward Rugh, Ellensburg, WA; Daryl Petrey, USDA-APHIS-PPQ, Ellensburg, WA; and The National Hay Association for assistance and support of this project. NR 21 TC 0 Z9 0 U1 2 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 100 EP 106 DI 10.1093/jee/tou032 PG 7 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700014 PM 26470109 ER PT J AU Nagoshi, RN Rosas-Garcia, NM Meagher, RL Fleischer, SJ Westbrook, JK Sappington, TW Hay-Roe, M Thomas, JMG Murua, GM AF Nagoshi, Rodney N. Rosas-Garcia, Ninfa M. Meagher, Robert L. Fleischer, Shelby J. Westbrook, John K. Sappington, Thomas W. Hay-Roe, Mirian Thomas, Jean M. G. Murua, Gabriela M. TI Haplotype Profile Comparisons Between Spodoptera frugiperda (Lepidoptera: Noctuidae) Populations From Mexico With Those From Puerto Rico, South America, and the United States and Their Implications to Migratory Behavior SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE fall armyworm; insect migration; cytochrome oxidase I; haplotypes ID FALL ARMYWORM LEPIDOPTERA; CORN-STRAIN POPULATIONS; HOST STRAINS; I GENE; FLORIDA; BRAZIL; TEXAS; IDENTIFICATION; ARGENTINA AB Fall armyworm [Spodoptera frugiperda (J. E. Smith)] is a major economic pest throughout the Western Hemisphere of maize, cotton, sorghum, and a variety of agricultural grasses and vegetable crops. Previous studies demonstrated extensive annual migrations occurring as far north as Canada from overwintering locations in southern Florida and Texas. In contrast, migratory behavior in the rest of the hemisphere is largely uncharacterized. Understanding the migration patterns of fall armyworm will facilitate efforts to predict the spread of pesticide resistance traits that repeatedly arise in this species and assess the consequences of changing climatic trends on the infestation range. Four independent fall armyworm colonies derived from widely separated populations in Mexico and two field collections were examined for their mitochondrial cytochrome oxidase I (COI) gene haplotypes and compared with other locations. The Mexico populations were most similar in their haplotype profile to those from Texas and South America, but also displayed some distinctive features. The data extend the haplotype distribution map in the Western Hemisphere and confirm that the previously observed regional differences in haplotype frequencies are stable over time. The Mexico collections were associated with haplotypes rarely found elsewhere, suggesting limited migratory interactions with foreign populations, including those in neighboring Texas. C1 [Nagoshi, Rodney N.; Meagher, Robert L.; Hay-Roe, Mirian; Thomas, Jean M. G.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Rosas-Garcia, Ninfa M.] Inst Politecn Nacl, Ctr Biotecnol Genom, Lab Biotecnol Ambiental, Reynosa, Mexico. [Fleischer, Shelby J.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Westbrook, John K.] USDA ARS, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. [Sappington, Thomas W.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Genet Lab, Ames, IA 50011 USA. [Murua, Gabriela M.] Secc Zool Agr, Estac Expt Agroind Obispo Colombres, San Miguel De Tucuman, Argentina. RP Nagoshi, RN (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM rodney.nagoshi@ars.usda.gov FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-67003-30209]; Comision de Operacion y Fomento de Actividades Academicas del Instituto Politecnico Nacional (COFAA-IPN) [SIP-IPN 20121610] FX This work was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Agreement No. 2011-67003-30209. We also thank project SIP-IPN 20121610 and Comision de Operacion y Fomento de Actividades Academicas del Instituto Politecnico Nacional (COFAA-IPN) for financial support. We are grateful to Dr. Steve Valles (USDA) and Dr. Sandra Allan (USDA) for helpful comments on the manuscript. NR 30 TC 4 Z9 4 U1 6 U2 30 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 135 EP 144 DI 10.1093/jee/tou044 PG 10 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700018 PM 26470113 ER PT J AU Sullivan, BT Allison, JD Goyer, RA Shepherd, WP AF Sullivan, Brian T. Allison, Jeremy D. Goyer, Richard A. Shepherd, William P. TI Sex Pheromone of the Baldcypress Leafroller (Lepidoptera: Tortricidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE semiochemical; attractant; chemical ecology; mate location; wetland ID FRUIT-TREE LEAFROLLER; ARCHIPS-ARGYROSPILUS; WALKER LEPIDOPTERA; LOUISIANA; MOTH; GOYERANA; SYSTEM; PEST AB The baldcypress leafroller, Archips goyerana Kruse (Lepidoptera: Tortricidae), is a specialist on Taxodium distichum (L.) Richard and has caused serious defoliation in swamps of southeastern Louisiana, accelerating decline of baldcypress forests concurrently suffering from nutrient depletion, prolonged flooding, and saltwater intrusion. We investigated the composition of the sex pheromone of this species. Coupled gas chromatography-electroantennographic detection (GC-EAD) analyses indicated that male antennae were sensitive to four compounds [(Z)-11-tetradecenyl acetate (Z11-14:OAc), (E)-11-tetradecenyl acetate (E11-14:OAc), (Z)-9-tetradecenyl acetate (Z9-14:OAc), and (Z)-11-tetradecen-1-ol (Z11-14:OH)] present in female abdominal tip extracts in an approximately 100:1.5:0.6:10 ratio. In trapping trials performed in a cypress-tupelo swamp in southeastern Louisiana, moths were attracted to blends of these four components presented in approximately the female-produced ratios. Elimination of Z11-14:OH had no impact on moth response, whereas elimination of any of the three acetates strongly reduced or eliminated attraction. A blend in which the E11:Z11 ratio of 14:OAc was 5:100 was much less attractive than the same blend with the female produced ratio of 1.5:100. A. goyerana is closely related to the sympatric species Archips argyrospilus (Walker) with which it was previously synonymous. Our data revealed differences between the pheromone composition of A. goyerana and that reported for A. argyrospilus, which could account for the apparent absence of cross-attraction between these species. We conclude that a lure containing a 100:1.5:0.6 ratio of Z11-14:OAc, E11-14:OAc, and Z9-14:OAc has the potential to be used in traps to detect and measure A. goyerana populations and thereby monitor an important biotic factor contributing to the loss of coastal baldcypress forests. C1 [Sullivan, Brian T.; Shepherd, William P.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Allison, Jeremy D.] Nat Resources Canada, Great Lakes Forestry Ctr, Ottawa, ON P6A 2E5, Canada. [Goyer, Richard A.] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. RP Sullivan, BT (reprint author), US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. EM briansullivan@fs.fed.us NR 20 TC 0 Z9 0 U1 4 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 166 EP 172 DI 10.1093/jee/tou018 PG 7 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700022 PM 26470117 ER PT J AU Fettig, CJ Munson, AS Reinke, M Mafra-Neto, A AF Fettig, Christopher J. Munson, A. Steven Reinke, Michael Mafra-Neto, Agenor TI A Novel Semiochemical Tool for Protecting Pinus contorta From Mortality Attributed to Dendroctonus ponderosae (Coleoptera: Curculionidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE lodgepole pine; mountain pine beetle; SPLAT Verb; tree protection; verbenone ID MOUNTAIN PINE; LODGEPOLE PINE; BEETLE INFESTATIONS; HOPKINS COLEOPTERA; UNITED-STATES; BARK BEETLES; SCOLYTIDAE; VERBENONE; ATTACK; WESTERN AB Verbenone (4,6,6-trimethylbicyclo[3.1.1]hept-3-en-2-one) is an antiaggregant of the mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), a notable forest insect capable of causing extensive levels of tree mortality in western North America. Several formulations of verbenone are registered for tree protection, but failures in efficacy are not uncommon, particularly when applied during large infestations. A formulation of (-)-verbenone was developed (Specialized Pheromone & Lure Application Technology [SPLAT] Verb, ISCA Technologies Inc., Riverside, CA) and evaluated for protecting individual lodgepole pine, Pinus contorta Douglas ex Loudon, and small stands of P. contorta from mortality attributed to D. ponderosae. SPLAT Verb applied to individual P. contorta resulted in complete tree protection, while 93.3% mortality occurred in the untreated controls. Significantly fewer P. contorta were killed by D. ponderosae within 0.041-ha circular plots surrounding P. contorta treated with SPLAT Verb compared with the untreated control. In a second study, a smaller percentage of P. contorta were colonized and killed on 0.4-ha square plots treated with SPLAT Verb compared with the untreated control. No significant differences in levels of tree mortality were observed between the untreated control and another formulation of verbenone (7-g pouch) or between the 7-g pouch and SPLAT Verb. In a trapping bioassay, no significant differences were observed among captures in multiple-funnel traps at 1, 2, or 4 m from the point of release of SPLAT Verb. Significantly fewer D. ponderosae were collected at 1 and 2 m compared with 8 m. Significantly more D. ponderosae were captured at the farthest distance evaluated (16 m) than at any other distance. Our data indicate that SPLAT Verb is effective for protecting individual P. contorta and small stands of P. contorta from mortality attributed to D. ponderosae at moderate doses. The high levels of tree protection observed are attributed to the ability of applying release points (dollops) at high densities, and a larger zone of inhibition than reported for other formulations of verbenone. SPLAT Verb was registered by the U.S. Environmental Protection Agency for use on pines, Pinus spp., in 2013. C1 [Fettig, Christopher J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Munson, A. Steven] US Forest Serv, USDA, Forest Hlth Protect, Ogden, UT 84403 USA. [Reinke, Michael; Mafra-Neto, Agenor] ISCA Technol Inc, Riverside, CA 92507 USA. RP Fettig, CJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. EM cfettig@fs.fed.us FU USDA Small Business Innovation Research (SBIR) grant [2012-33610-19488]; USDA Forest Service Pesticide Impact Assessment Program (FS-PIAP) grant [R1/4-2012-02]; USDA Forest Service Forest Health Technology Enterprise Team grant [FHTET-13]; ISCA Technologies Inc. [12-CO-39-026] FX We thank R. Cruz, V. DeBlander, L. Dunning, J. Guyon, C. Hayes, L. Hebertson, J. Hoffman, C. Jorgensen, B. Meyerson, J. Neumann, B. Rabaglia, and D. Reboletti (Forest Health Protection, USDA Forest Service); F. de Lame, B. Roble, J. Self, L. Stoltman, and D. Zeni (ISCA Technologies Inc.); C. Carvalho, C. Dabney, and S. McKelvey (Pacific Southwest Research Station, USDA Forest Service); and L. Mortensen (Pacific Northwest Research Station, USDA Forest Service) for technical assistance. In addition, we thank A. Mendonca and H. Whitlach (Bridger-Teton National Forest), D. Duehren, and W. Beck (Caribou-Targhee National Forest); and J. Gibson (Uinta-Wasatch-Cache National Forest) for providing access to study sites. This research was supported, in part, by a USDA Small Business Innovation Research (SBIR) grant (2012-33610-19488) to A.M.-N., a USDA Forest Service Pesticide Impact Assessment Program (FS-PIAP) grant (R1/4-2012-02) to C.J.F., A.S.M., N.E.G., and A.M.-N., a USDA Forest Service Forest Health Technology Enterprise Team grant (FHTET-13) to C.J.F., A.S.M., and A.M.-N., and a collection agreement (12-CO-39-026) from ISCA Technologies Inc. to the Pacific Southwest Research Station. NR 51 TC 0 Z9 0 U1 1 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 173 EP 182 DI 10.1093/jee/tou038 PG 10 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700023 PM 26470118 ER PT J AU Miller, DR Dodds, KJ Hoebeke, ER Poland, TM Willhite, EA AF Miller, D. R. Dodds, K. J. Hoebeke, E. R. Poland, T. M. Willhite, E. A. TI Variation in Effects of Conophthorin on Catches of Ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae) in Ethanol-Baited Traps in the United States SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Xyleborini; conophthorin; semiochemical; exotic; adventive ID XYLOSANDRUS-GERMANUS COLEOPTERA; MULTIPLE-FUNNEL TRAPS; PINE-CONE BEETLE; AGGREGATION PHEROMONE; BARK BEETLES; GNATHOTRICHUS-SULCATUS; SPECIES COMPOSITION; MATING SYSTEMS; VOLATILES; VERBENONE AB In 2013, we examined the effects of conophthorin on flight responses of ambrosia beetles (Coleoptera: Curculionidae: Scolytinae) to multiple-funnel traps baited with ethanol in Georgia, Michigan, New Hampshire, and Oregon. Adventive species (=exotic, nonnative, immigrant, introduced) accounted for 91.4% of total catches of ambrosia beetles. Conophthorin increased catches of Xyleborinus saxesenii (Ratzeburg) in Georgia, New Hampshire, and Oregon. Catches of Cyclorhipidion pelliculosum (Eichhoff) were increased by conophthorin in New Hampshire but not in Michigan. In Oregon, conophthorin decreased catches of Xylosandrus germanus (Blandford) to ethanol-baited traps but not in Michigan and New Hampshire. In Georgia, conophthorin increased catches of Gnathotrichus materiarius (Fitch), Xyleborus spp., and Xylosandrus crassiusculus (Motschulsky) but decreased catches of Cnestus mutilatus (Blandford), Dryoxylon onoharaensum (Murayama), and Cyclorhipidion bodoanum (Reitter). Conophthorin had no effect on catches of Ambrosiophilus atratus (Eichhoff), Anisandrus dispar (F.), Anisandrus sayi (Hopkins), Gnathotrichus sulcatus (Leconte), Monarthrum fasciatum (Say), Monarthrum mali (Fitch), and Xylosandrus compactus (Eichhoff). Attraction of the bark beetle, Hypothenemus rotundicollis (Eichhoff), was interrupted by conophthorin in Georgia. Our results suggest that adding conophthorin lures to traps baited with ethanol may have utility in detection programs in North America and overseas. However, traps baited with ethanol alone should also be used due to interruption in attraction for some species of ambrosia beetles. C1 [Miller, D. R.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. [Dodds, K. J.] US Forest Serv, USDA, Forest Hlth Protect, Durham, NH 03824 USA. [Hoebeke, E. R.] Univ Georgia, Georgia Museum Nat Hist, Athens, GA 30602 USA. [Hoebeke, E. R.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Poland, T. M.] US Forest Serv, USDA, No Res Stn, Lansing, MI 48910 USA. [Willhite, E. A.] US Forest Serv, USDA, Forest Hlth Protect, Sandy, OR 97055 USA. RP Miller, DR (reprint author), US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. EM dmiller03@fs.fed.us NR 66 TC 3 Z9 3 U1 7 U2 42 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 183 EP 191 DI 10.1093/jee/tou043 PG 9 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700024 PM 26470119 ER PT J AU Jenkins, DA Hall, DG Goenaga, R AF Jenkins, David A. Hall, David G. Goenaga, Ricardo TI Diaphorina citri (Hemiptera: Liviidae) Abundance in Puerto Rico Declines with Elevation SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Asian citrus psyllid; Citrus; elevation; Puerto Rico; Huanglongbing ID CANDIDATUS LIBERIBACTER ASIATICUS; COFFEE BERRY BORER; GREENING DISEASE; PSYLLIDAE; BIOLOGY; FLORIDA; PERFORMANCE; CHEMISTRY; RADIATION; ALTITUDE AB Diaphorina citri Kuwayama is the primary vector of Huanglongbing, the most devastating disease of citrus. Diaphorina citri populations in Puerto Rico were monitored with yellow sticky traps on citrus trees or other psyllid host plants at different elevations, ranging from 10 to 880 m above sea level. Trapping was conducted in March through May of 2013 and 2014 when psyllid populations usually are highest. Population levels of Diaphorina citri, based on the trapping data, varied among the sites, and there was a strong trend in both years for decreasing psyllid abundance with increased elevation based on the number of psyllids captured on traps and the proportion of trees shown to be infested. No psyllids were collected at an elevation of >600 m. Reduced populations at higher elevations could be a consequence of differences in temperature, air pressure, oxygen levels, ultraviolet light, or other factors alone or in combination. We discuss our results as they pertain to management of Diaphorina citri and Huanglongbing. C1 [Jenkins, David A.; Goenaga, Ricardo] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Hall, David G.] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. RP Jenkins, DA (reprint author), USDA ARS, Trop Agr Res Stn, 2200 Ave PA Campos,Ste 201, Mayaguez, PR 00680 USA. EM david.jenkins@ars.usda.gov NR 35 TC 1 Z9 1 U1 1 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 252 EP 258 DI 10.1093/jee/tou050 PG 7 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700032 PM 26470127 ER PT J AU Hanavan, RP Pontius, J Hallett, R AF Hanavan, Ryan P. Pontius, Jennifer Hallett, Richard TI A 10-Year Assessment of Hemlock Decline in the Catskill Mountain Region of New York State Using Hyperspectral Remote Sensing Techniques SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE IPM; biological control; decision support; forest decline; hemlock woolly adelgid ID ADELGID ADELGES-TSUGAE; EASTERN-UNITED-STATES; VEGETATION INDEXES; CHLOROPHYLL-A; COLEOPTERA DERODONTIDAE; SPECTRAL REFLECTANCE; EUCALYPTUS LEAVES; HIGHER-PLANTS; MAPLE LEAVES; BROAD-BAND AB The hemlock woolly adelgid is a serious pest of Eastern and Carolina hemlock in the eastern United States. Successfully managing the hemlock resource in the region depends on careful monitoring of the spread of this invasive pest and the targeted application of management options such as biological control, chemical, or silvicultural treatments. To inform these management activities and test the applicability of a landscape-scale remote sensing effort to monitor hemlock condition, hyperspectral collections, and concurrent ground-truthing in 2001 and 2012 of hemlock condition were compared with field metrics spanning a 10-yr survey in the Catskills region of New York. Fine twig dieback significantly increased from 9 to 15% and live crown ratio significantly decreased from 67 to 56% in 2001 and 2012, respectively. We found a significant shift from 59% "healthy" hemlock in 2001 to only 16% in 2012. However, this shift from healthy to declining classifications was mostly a shift to decline class 2 "early decline". These results indicate that while there has been significant increase in decline symptoms as measured in both field and remote sensing assessments, a majority of the declining areas identified in the resulting spatial coverages remain in the "early decline" category and widespread mortality has not yet occurred. While this slow decline across the region stands in contrast to many reports of mortality within 10 yr, the results from this work are in line with other long-term monitoring studies and indicate that armed with the spatial information provided here, continued management strategies can be focused on particular areas to help control the further decline of hemlock in the region. C1 [Hanavan, Ryan P.] USDA, Forest Serv, Northeastern Area State & Private Forestry, Durham, NH 03824 USA. [Pontius, Jennifer] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Hallett, Richard] USDA, Forest Serv, No Res Stn, Durham, NH 03824 USA. RP Hanavan, RP (reprint author), USDA, Forest Serv, Northeastern Area State & Private Forestry, 271 Mast Rd, Durham, NH 03824 USA. EM rhanavan02@fs.fed.us FU United States Department of Agriculture (USDA) Forest Service Hemlock Woolly Adelgid Initiative FX This work would not have been possible without the image acquisition from SpecTIR and field assistance from the New York State Department of Environmental Conservation (NYS DEC): Bob McKenzie, Jeff Wiegert, Jeff Rider, Jerry Carlson, Mike Callan, and Scott McDonnell; and the United States Forest Service (USFS): Garret Dubois, Justin Williams, Mike Bohne, and Mike Simmons. We are also grateful to Kevin Dodds (USFS) and David Orwig (Harvard Forest) for providing helpful comments to improve this manuscript. Funding for this work was provided by the United States Department of Agriculture (USDA) Forest Service Hemlock Woolly Adelgid Initiative. NR 71 TC 1 Z9 1 U1 6 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 339 EP 349 DI 10.1093/jee/tou015 PG 11 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700043 PM 26470138 ER PT J AU Kendra, PE Niogret, J Montgomery, WS Deyrup, MA Epsky, ND AF Kendra, Paul E. Niogret, Jerome Montgomery, Wayne S. Deyrup, Mark A. Epsky, Nancy D. TI Cubeb Oil Lures: Terpenoid Emissions, Trapping Efficacy, and Longevity for Attraction of Redbay Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Xyleborus glabratus; laurel wilt; manuka oil; alpha-copaene; alpha-cubebene ID LAUREL WILT DISEASE; SOUTHEASTERN UNITED-STATES; XYLEBORUS-GLABRATUS; ELECTROANTENNOGRAM RESPONSES; RAFFAELEA-LAURICOLA; MANUKA OIL; PHOEBE OIL; AVOCADO; LAURACEAE; SYMBIONT AB Redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is an exotic wood borer and the primary vector of Raffaelea lauricola, a symbiotic fungus that causes laurel wilt. This lethal disease has decimated native redbay [Persea borbonia (L.) Sprengel] and swampbay [Persea palustris (Rafinesque) Sargent] throughout southeastern U.S. forests, and currently threatens avocado (Persea americana Miller) in Florida. To curtail the spread of laurel wilt, effective attractants are needed for early detection of the vector. Phoebe oil lures were the best known attractant for Xyleborus glabratus, but they are no longer available. The current detection system uses manuka oil lures, but previous research indicated that manuka lures have a short field life in Florida. Recently, cubeb oil was identified as a new attractant for Xyleborus glabratus, and cubeb bubble lures are now available commercially. This study compared trapping efficacy and field longevity of cubeb and manuka lures with phoebe lures that had been in storage since 2010 over a 12-wk period in south Florida. In addition, terpenoid emissions were quantified from cubeb and manuka lures aged outdoors for 12 wk. Captures were comparable with all three lures for 3 wk, but by 4 wk, captures with manuka were significantly less. Equivalent captures were obtained with cubeb and phoebe lures for 7 wk, but captures with cubeb were significantly greater from 8 to 12 wk. Our results indicate that cubeb bubble lures are the most effective tool currently available for detection of Xyleborus glabratus, with a field life of 3 months due to extended low release of attractive sesquiterpenes, primarily alpha-copaene and alpha-cubebene. C1 [Kendra, Paul E.; Niogret, Jerome; Montgomery, Wayne S.; Epsky, Nancy D.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Deyrup, Mark A.] Archbold Biol Stn, Lake Placid, FL 33862 USA. RP Kendra, PE (reprint author), USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM paul.kendra@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; Florida Avocado Administrative Committee FX We are grateful to Hilary Swain for providing laboratory space, lodging, and access to field sites at Archbold Biological Station (Lake Placid, FL); to David Wakarchuk (Synergy Semiochemicals Corp.; Burnaby, BC, Canada) for providing cubeb bubble lures for field and laboratory evaluation; and to Daniel Carrillo (University of Florida, Homestead), Charles Stuhl (USDA-ARS, Gainesville, FL), and three anonymous reviewers for providing helpful suggestions with the manuscript. We also acknowledge the Florida Department of Environmental Protection for granting permission to conduct research at Highlands Hammock State Park, Sebring, FL (Research/Collecting Permit #01281314). This work was supported, in part, by funding from the USDA-ARS National Plant Disease Recovery System and the Florida Avocado Administrative Committee. NR 46 TC 7 Z9 7 U1 2 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD FEB PY 2015 VL 108 IS 1 BP 350 EP 361 DI 10.1093/jee/tou023 PG 12 WC Entomology SC Entomology GA CC0NX UT WOS:000350033700044 PM 26470139 ER PT J AU Li, JY Song, W Barth, MM Zhuang, H Zhang, WL Zhang, LW Wang, L Lu, WH Wang, ZY Han, X Li, Q AF Li, Jingyan Song, Wei Barth, M. Margaret Zhuang, Hong Zhang, Wenlong Zhang, Lanwei Wang, Li Lu, Weihong Wang, Zhenyu Han, Xue Li, Qi TI EFFECT OF MODIFIED ATMOSPHERE PACKAGING (MAP) ON THE QUALITY OF SEA BUCKTHORN BERRY FRUITS DURING POSTHARVEST STORAGE SO JOURNAL OF FOOD QUALITY LA English DT Article ID SHELF-LIFE; ANTIOXIDANT ACTIVITY; LOW-TEMPERATURE; BLUEBERRIES; EXTRACTS; FIRMNESS AB The effect of modified atmosphere packaging (MAP) on the quality of the berry fruits of sea buckthorn (SBT) during refrigerated storage was investigated. SBT berries were packaged in 160 and 525 oxygen transmission rate (OTR) films or in vented clamshell containers (air control) and stored at 10C for 10 days. Changes in the quality of the berries were determined by the evaluation of their headspace composition, moisture content, dry weight, pH level, color, total soluble solids, texture and antioxidant activity. The results indicated that the 160 OTR film presented good competency to maintain a proper atmosphere for storage. Both the 160 and 525 OTR films showed better capability for retaining the firm and springy texture of the berries, as well as their weight. The total soluble solids of 160 OTR packed fruits remained a stable value. The pH of SBT berries packed in different films and control samples increased in general during storage compared with the initial pH of fruits. The antioxidant activity of the berries was higher in MAP samples, especially those in the 160 OTR packages. Practical ApplicationsThis is the first study on the storage characteristics of berry fruit of sea buckthorn (SBT) under modified atmosphere packaging conditions. Film of 160 oxygen transmission rate (OTR) effectively maintained the initial quality parameters and antioxidant activity of berries by slowing down the respiration intensity. It may be because 160 OTR provided excellent CO2/O-2 exchange performance to supply a desired gaseous environment of berry fruit. Thus, 160 OTR could be used as a potential packaging film for SBT berry on maintaining fruit quality and antioxidant activity. Furthermore, 160 OTR to different food systems of vegetables, mushrooms and juicy fruits can greatly maintain the nutritional value, product quality and fresh supply. The results of the present study can also serve as an assistive technology combined with other preservation techniques such as ice temperature, controlled atmosphere storage and so on to achieve desirable storage performance. C1 [Li, Jingyan; Song, Wei; Zhang, Wenlong; Zhang, Lanwei; Wang, Li; Lu, Weihong; Wang, Zhenyu; Han, Xue; Li, Qi] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China. [Li, Jingyan] Northwest A&F Univ, Sch Food Sci & Engn, Yangling 712100, Peoples R China. [Barth, M. Margaret] Univ Illinois, Responsible Source Ltd, Champaign, IL 61820 USA. [Zhuang, Hong] Univ Georgia, USDA, ARS, Athens, GA 30602 USA. RP Zhang, LW (reprint author), Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150090, Peoples R China. EM zhanglw@hit.edu.cn FU Ministry of Science and Technology of the People's Republic of China [2011AA100805-1] FX This work was financially supported by the Ministry of Science and Technology of the People's Republic of China (2011AA100805-1). NR 31 TC 0 Z9 0 U1 7 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0146-9428 EI 1745-4557 J9 J FOOD QUALITY JI J. Food Qual. PD FEB PY 2015 VL 38 IS 1 BP 13 EP 20 DI 10.1111/jfq.12118 PG 8 WC Food Science & Technology SC Food Science & Technology GA CB8PV UT WOS:000349893900002 ER PT J AU Duarte, GF Rodrigues, J Fernandes, EKK Humber, RA Luz, C AF Duarte, Glennyha F. Rodrigues, Juscelino Fernandes, Everton K. K. Humber, Richard A. Luz, Christian TI New insights into the amphibious life of Biomphalaria glabrata and susceptibility of its egg masses to fungal infection SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Mollusc; Water film; Test device; Beauveria; Metarhizium ID LABORATORY CONDITIONS; CERRADO ECOSYSTEM; GASTROPOD; PHENOLOXIDASE; PLANORBIDAE; MOLLUSKS AB The air-breathing snail Biomphalaria glabrata proliferates in stagnant freshwater, and nothing is known about the survival of eggs in intermittently (rather than perpetually) wet habitats. In the present study their egg masses matured, and juveniles subsequently eclosed and were mobile in a stable water film of transitory habitats simulated by two different simple test devices described here. The viability of eggs maintained in an unstable film however, was diminished. The maturation of egg masses in a water film or in water was significantly prevented by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. The efficiency depended on the fungal propagule and test environment. Hyphal bodies were more effective against egg masses than conidia. This appears to be a first report of activity of either entomopathogen against a mollusc. Both devices offer accurate and reproducible conditions to test both biological questions and the effects of substances or pathogens against B. glabrata egg masses in water films. (C) 2015 Elsevier Inc. All rights reserved. C1 [Duarte, Glennyha F.; Rodrigues, Juscelino; Fernandes, Everton K. K.; Luz, Christian] Univ Fed Goias, IPTSP, DMIPP, BR-74001970 Goiania, Go, Brazil. [Humber, Richard A.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Luz, C (reprint author), Univ Fed Goias, IPTSP, DMIPP, CP 131, BR-74001970 Goiania, Go, Brazil. EM wchrisluz@hotmail.com RI Fernandes, Everton/I-4890-2012 OI Fernandes, Everton/0000-0001-7062-3295 FU Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil); National Council of Scientific and Technological Development (CNPq, Brazil) FX GFD received a scholarship from the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil) and JR and CL from the National Council of Scientific and Technological Development (CNPq, Brazil); the research received no specific grant from any funding agency, commercial or not-for-profit sectors. Authors declare to have no conflict of interest. NR 29 TC 2 Z9 2 U1 0 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD FEB PY 2015 VL 125 BP 31 EP 36 DI 10.1016/j.jip.2014.12.013 PG 6 WC Zoology SC Zoology GA CC0FZ UT WOS:000350012500005 PM 25576771 ER PT J AU Faria, M Lopes, RB Souza, DA Wraight, SP AF Faria, Marcos Lopes, Rogerio Biaggioni Souza, Daniela Aguiar Wraight, Stephen P. TI Conidial vigor vs. viability as predictors of virulence of entomopathogenic fungi SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Entomopathogenic fungi; Beauveria bassiana s.l.; Biopesticides; Mycoinsecticides; Germination protocol; Quality control ID BEAUVERIA-BASSIANA CONIDIA; METARHIZIUM-ANISOPLIAE; PAECILOMYCES-FUMOSOROSEUS; GERMINATION; INFECTIVITY AB We tested the hypothesis that debilitated conidia exhibiting slow-germination (requiring > 16 h to germinate) are less virulent than vigorous conidia exhibiting fast germination (requiring <= 16 h to germinate). Preparations of Beauveria bassiana s.l. strain CG 1027 with variable ratios of vigorous to debilitated conidia were assayed against third-instar larvae of Spodoptera frugiperda. As the proportion of debilitated conidia in test preparations increased, LC50 expressed in terms of total viable conidia increased, while LC50 expressed solely in terms of vigorous conidia remained constant, indicating that vigorous conidia were responsible for nearly all mortality observed in the assays. Larvae treated with conidia from low-quality batches (with high proportions of debilitated conidia) survived consistently longer than those treated with comparable doses of conidia from high-quality batches. These results confirm our previous hypotheses that inclusion of debilitated conidia in viability assessments can lead to overestimation of the quality (potency) of mycoinsecticide preparations and support our recommendation for use of short incubation periods for assessing viability whenever viability is relied upon as an indicator of product quality. (C) 2015 Published by Elsevier Inc. C1 [Faria, Marcos; Lopes, Rogerio Biaggioni; Souza, Daniela Aguiar] EMBRAPA Genet Resources & Biotechnol, BR-70770917 Brasilia, DF, Brazil. [Wraight, Stephen P.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Wraight, SP (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM steve.wraight@ars.usda.gov RI Lopes, Rogerio/L-3534-2013; Faria, Marcos/E-1446-2012 OI Lopes, Rogerio/0000-0003-4756-878X; Faria, Marcos/0000-0001-9420-0916 FU EMBRAPA (Brazilian Corporation for Agricultural Research); Brazilian National Research Council (CNPq) FX The research described in this publication was funded by EMBRAPA (Brazilian Corporation for Agricultural Research) and the Brazilian National Research Council (CNPq). We thank Dayane Garcia for assistance in bioassays. NR 16 TC 4 Z9 4 U1 4 U2 22 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD FEB PY 2015 VL 125 BP 68 EP 72 DI 10.1016/j.jip.2014.12.012 PG 5 WC Zoology SC Zoology GA CC0FZ UT WOS:000350012500010 PM 25573792 ER PT J AU Laussen, JC Chale, A Hau, C Fielding, RA White, DK AF Laussen, Jonathan C. Chale, Angela Hau, Cynthia Fielding, Roger A. White, Daniel K. TI DOES PHYSICAL ACTIVITY CHANGE AFTER PROGRESSIVE RESISTANCE EXERCISE IN FUNCTIONALLY LIMITED OLDER ADULTS? SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Letter ID MOBILITY; LIMITATIONS; PERFORMANCE; EFFICACY; ELDERS; MASS; LIFE; MEN C1 [Laussen, Jonathan C.; Hau, Cynthia; Fielding, Roger A.; White, Daniel K.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chale, Angela] Montclair State Univ, Dept Hlth & Nutr Sci, Montclair, NJ USA. [White, Daniel K.] Univ Delaware, Dept Phys Therapy, Newark, DE USA. [White, Daniel K.] Boston Univ, Dept Phys Therapy & Athlet Training, Boston, MA 02215 USA. RP Laussen, JC (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. FU Dairy Research Institute; Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679]; Boston Nutrition/Obesity Research Center [DK046200]; Boston Rehabilitation Outcomes Center [R24HD0065688]; ACR/RRF Bridge Funding Award; Foundation for Physical Therapy Geriatric Research Grant; Department of Agriculture [58-1950-0-014]; [DK007651] FX This work was supported by the Dairy Research Institute (RAF), the Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679 to RAF), the Boston Nutrition/Obesity Research Center (DK046200 to RAF), the Boston Rehabilitation Outcomes Center (R24HD0065688 to RAF and DKW), ACR/RRF Bridge Funding Award (DKW), the Foundation for Physical Therapy Geriatric Research Grant (DKW), a postdoctoral training grant (DK007651 to AC), and the Department of Agriculture (Agreement 58-1950-0-014). Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the Department of Agriculture. NR 10 TC 2 Z9 2 U1 0 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0002-8614 EI 1532-5415 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD FEB PY 2015 VL 63 IS 2 BP 392 EP 393 DI 10.1111/jgs.13270 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA CB8PP UT WOS:000349893300031 PM 25688616 ER PT J AU Peredo, AM Buentello, A Gatlin, DM Hume, ME AF Peredo, Anjelica M. Buentello, Alejandro Gatlin, Delbert M., III Hume, Michael E. TI Evaluation of a Dairy-Yeast Prebiotic in the Diet of Juvenile Nile Tilapia, Oreochromis niloticus SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID DRUM SCIAENOPS-OCELLATUS; X MORONE-SAXATILIS; GRADIENT GEL-ELECTROPHORESIS; HUMAN COLONIC MICROBIOTA; RED DRUM; BREWERS-YEAST; RAINBOW-TROUT; IN-VITRO; NONSPECIFIC IMMUNITY; GROWTH-PERFORMANCE AB Two feeding trials were conducted to evaluate the effects of dietary supplementation with the prebiotic GroBiotic-A on juvenile Nile tilapia, Oreochromis niloticus. Juvenile fish in both trials received a basal diet composed of practical and semipurified ingredients or the basal diet supplemented with GroBiotic-A at either 1 or 2% of dry weight. Trial 1 (8wk), was conducted with juvenile fish initially averaging 4.3g/fish in which their performance indices, nonspecific humoral and cellular immune responses, and intestinal microbiota were characterized. In that trial, only survival was significantly affected by dietary treatment with the greatest survival (98.7%) observed for fish fed the diet containing 2% GroBiotic-A and significantly (P<0.05) lower survival (86.9%) for fish fed the diet containing 1% GroBiotic-A (GBA) and intermediate survival (90.7%) for fish fed the basal diet. Trial 2 (5wk), was conducted with larger juveniles initially averaging 6.5g/fish to provide a more in-depth assessment of cellular immune responses, intestinal morphometric characteristics, and disease resistance of tilapia fed the three experimental diets. Fish fed the diet with 2% GroBiotic-A exhibited significantly (P<0.05) increased production of extracellular superoxide anion by macrophages and a distinct microbial community within the intestine compared to fish fed the other diets. No significant differences were seen in survival after controlled exposure to Streptococcus iniae although fish fed the diet with 2% GBA tended to experience reduced mortality (12.5%) compared to those fed the basal diet (35%). Thus, based on the results from both trials, supplementation of 2% GroBiotic-A generally improved survival, altered gut microbiota, and enhanced extracellular superoxide anion production of Nile tilapia. C1 [Peredo, Anjelica M.; Buentello, Alejandro; Gatlin, Delbert M., III] Texas A&M Univ, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA. [Hume, Michael E.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Gatlin, DM (reprint author), Texas A&M Univ, Dept Wildlife & Fisheries Sci, 216 Heep Lab Bldg,2258 TAMUS, College Stn, TX 77843 USA. EM d-gatlin@tamu.edu FU National Center for Foreign Animal and Zoonotic Disease Defense (FAZD Center); Hispanic Leaders in Agriculture and the Environment (HLAE) at Texas AM University FX The authors wish to thank the National Center for Foreign Animal and Zoonotic Disease Defense (FAZD Center) and the Hispanic Leaders in Agriculture and the Environment (HLAE) at Texas A&M University for A. M. P. M. S. fellowship. NR 39 TC 2 Z9 2 U1 2 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD FEB PY 2015 VL 46 IS 1 BP 92 EP 101 DI 10.1111/jwas.12170 PG 10 WC Fisheries SC Fisheries GA CC1WN UT WOS:000350136100010 ER PT J AU Castro, P Bushakra, JM Stewart, P Weebadde, CK Wang, D Hancock, JF Finn, CE Luby, JJ Lewers, KS AF Castro, P. Bushakra, J. M. Stewart, P. Weebadde, C. K. Wang, D. Hancock, J. F. Finn, C. E. Luby, J. J. Lewers, K. S. TI Genetic mapping of day-neutrality in cultivated strawberry SO MOLECULAR BREEDING LA English DT Article DE Linkage map; QTL map; Remontant; Everbearing; Photoperiod insensitivity ID OCTOPLOID FRAGARIA-VIRGINIANA; DIPLOID FRAGARIA; LINKAGE MAP; EVERBEARING STRAWBERRIES; QUANTITATIVE TRAITS; WILD STRAWBERRY; XANANASSA DUCH.; SEX-CHROMOSOME; INHERITANCE; MARKERS AB Day-neutrality is a highly desirable trait in strawberry (Fragaria L.)-breeding programs worldwide due to its importance in extending the harvest season in commercial production. Day-neutral genotypes are photoperiod insensitive and will initiate flowers under any photoperiod conditions as long as temperatures are moderate (below 30/26 degrees C day/night). In the current study, the inheritance of dayneutrality was investigated using an F-1 population derived from the cross 'Tribute' 9 'Honeoye'. The day-neutral trait was scored qualitatively [day-neutral (DN) or short-day (SD)], and quantitatively (number of weeks of flowering). When qualitatively scored in five locations, the DN trait fit a 1: 1 segregation ratio in the hot summers of Maryland (MD), Minnesota (MN) and Michigan (MI). Segregation was skewed toward additional DN progeny in the cooler summers of California (CA) and Oregon (OR). Regardless of evaluation location, the trait was mapped on linkage group IV-T-1 of the 'Tribute' map near the markers ChFaM148-184T and ChFaM011-163T. Quantitative-trait loci (QTL) for number of weeks of flowering in MD and CA and for stolon or 'runner' production in MN, MI and OR also were identified on linkage group IV-T-1 of the 'Tribute' map. These QTL were significantly associated with the qualitatively scored trait and its closely linked molecular markers. The marker ChFaM148-184T explained 63.8 % of the total phenotypic variation for the number of weeks of flowering for MD and 32.4 % of the total phenotypic variation for runner production in MN. Consequently, it was hypothesized that a single major gene or tightly linked cluster of genes in coupling control day-neutrality in this population. The results found in the current study suggest that day-neutrality can be qualitatively scored at least in the locations where the temperatures do not allow SD plants to behave as DN. C1 [Castro, P.; Lewers, K. S.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. [Bushakra, J. M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Stewart, P.] Driscoll Strawberry Associates Inc, Watsonville, CA 95077 USA. [Weebadde, C. K.; Wang, D.; Hancock, J. F.] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Finn, C. E.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Luby, J. J.] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. RP Lewers, KS (reprint author), USDA ARS, Genet Improvement Fruits & Vegetables Lab, Bldg 010A BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Patricia.Castro@ars.usda.gov; Kim.Lewers@ars.usda.gov FU USDA Cooperative State Research, Education and Extension Service-National Research Initiative-Plant Genome Program [2005-35300-15468]; USDA-ARS [1245-21220-254-00, 5358-21220-002-00]; Driscoll Strawberry Associates, Inc.; Michigan State University; University of Minnesota FX This project was partially funded by the USDA Cooperative State Research, Education and Extension Service-National Research Initiative-Plant Genome Program (Award # 2005-35300-15468), USDA-ARS Projects 1245-21220-254-00 and 5358-21220-002-00, Driscoll Strawberry Associates, Inc., Michigan State University and the University of Minnesota. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or any of the other agencies involved in this research. NR 56 TC 6 Z9 6 U1 7 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2015 VL 35 IS 2 AR 79 DI 10.1007/s11032-015-0250-4 PG 16 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CB6VR UT WOS:000349765300023 ER PT J AU Cheng, P Holdsworth, W Ma, Y Coyne, CJ Mazourek, M Grusak, MA Fuchs, S McGee, RJ AF Cheng, Peng Holdsworth, William Ma, Yu Coyne, Clarice J. Mazourek, Michael Grusak, Michael A. Fuchs, Sam McGee, Rebecca J. TI Association mapping of agronomic and quality traits in USDA pea single-plant collection SO MOLECULAR BREEDING LA English DT Article DE Association mapping; Pisum sativum; Single nucleotide polymorphism; Marker-assisted selection; Pea single-plant collection ID GENOME-WIDE ASSOCIATION; PISUM-SATIVUM L.; FIELD PEA; CORE COLLECTION; LINKAGE DISEQUILIBRIUM; GENETIC DIVERSITY; ANTINUTRITIONAL FACTORS; CHEMICAL-COMPOSITION; POPULATION-STRUCTURE; MEDICAGO-TRUNCATULA AB Association mapping is an efficient approach for the identification of the molecular basis of agronomic traits in crop plants. For this purpose in pea (Pisum sativum L.), we genotyped and phenotyped individual lines of the single-plant-derived core collection of the USDA pea collection including accessions from 330 landraces and cultivars of Pisum sativum subsp. sativum var. sativum, 28 P. sativum subsp. elatius var. elatius, 16 P. sativum subsp. sativum var. arvense, four P. sativum subsp. elatius var. pumilio, three P. abyssinicum, two P. fulvum, and one P. sativum subsp. transcaucasicum. These 384 accessions were collected or donated from a total of 64 countries. The accessions were genotyped with 256 informative SNPs using a primer extension chemistry and matrix-assisted laser desorption/ionization (MALDI-TOF) mass spectrometry assay. Genetic structure analysis showed that the collection was structured into two main groups, corresponding roughly to the cultivated types/landraces and the more primitive form species and subspecies, with some intermediates. Linkage disequilibrium of pairwise loci and population structure of the collection were analyzed, and an association analysis between SNP genotypes and 25 valuable traits such as disease resistance, seed type/color, flower color, seed low molecular weight carbohydrate concentration, and seed mineral nutrient concentration was performed using a mixed linear model. A total of 71 marker-trait associations were detected as significant with 1-34 markers per trait based on the false discovery rate (FDR < 0.05). This study demonstrates the potential of using association mapping to identify markers for pea breeding. C1 [Cheng, Peng] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Holdsworth, William; Mazourek, Michael] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Ma, Yu] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Coyne, Clarice J.; Fuchs, Sam] ARS, USDA, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. [Grusak, Michael A.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [McGee, Rebecca J.] ARS, USDA, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. RP McGee, RJ (reprint author), ARS, USDA, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. EM rjmcgee@wsu.edu FU USA Dry Pea and Lentil Council FX This project was supported by the USA Dry Pea and Lentil Council. We thank Mr. Hun Huang Ooi for assistance with DNA plate organization and Ms. Sheri McGrew for taking care of the plants in the greenhouse. The Sequenom iPLEX assay was done in the USDA Wheat Genetics, Quality, Physiology, and Disease Research Unit. We thank Dr. Derick Jiwan and Dr. Deven See for their technical support on SNP calling and validation. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 71 TC 6 Z9 6 U1 7 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2015 VL 35 IS 2 AR 75 DI 10.1007/s11032-015-0277-6 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CB6VR UT WOS:000349765300019 ER PT J AU Kolmer, JA AF Kolmer, J. A. TI A QTL on chromosome 5BL in wheat enhances leaf rust resistance of Lr46 SO MOLECULAR BREEDING LA English DT Article DE Adult plant resistance; Brown rust; Puccinia triticina; Triticum aestivum ID WINTER-WHEAT; COMMON WHEAT; STRIPE RUST; GENETICS; CULTIVARS AB Combinations of effective leaf rust resistance genes that condition resistance to many leaf rust races are key to the development of wheat cultivars with long-lasting resistance to leaf rust caused by Puccinia triticina. A population of F-6 recombinant inbred lines (RILs) derived from the cross 'Thatcher*3/Americano 25e' was evaluated for segregation of adult plant leaf rust resistance in three field plot tests and in a greenhouse test. A genetic map was constructed with 243 diversity array technology markers. Significant effects for reduction in leaf rust severity were found on chromosomes 1BL and 5BL. The sequence-tagged site marker csLV46, closely linked to Lr46, mapped to the logarithm of odds peak on chromosome 1BL, indicating that the RILs were likely segregating for this adult plant resistance gene. Lines with csLV46 had an average leaf rust severity of 35 % in the four tests. RILs with the resistance on chromosome 5BL designated as QLr. cdl-5BL had an average leaf rust severity of 47 %. RILs with both csLV46 and QLr. cdl-5BL had an average leaf rust severity of 22 %, close to the Thatcher*2/Americano 25e parental line. Lines that lacked both csLV46 and QLr-5BL had an average severity of 56 %. The QLr. cdl-5BL region enhanced the leaf resistance conditioned by Lr46 in an additive manner. C1 USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM jim.kolmer@ars.usda.gov NR 26 TC 4 Z9 4 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2015 VL 35 IS 2 AR 74 DI 10.1007/s11032-015-0274-9 PG 8 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CB6VR UT WOS:000349765300018 ER PT J AU Lee, S Freewalt, KR McHale, LK Song, QJ Jun, TH Michel, AP Dorrance, AE Mian, MAR AF Lee, Sungwoo Freewalt, Keith R. McHale, Leah K. Song, Qijian Jun, Tae-Hwan Michel, Andrew P. Dorrance, Anne E. Mian, M. A. Rouf TI A high-resolution genetic linkage map of soybean based on 357 recombinant inbred lines genotyped with BARCSoySNP6K SO MOLECULAR BREEDING LA English DT Article DE Soybean; Genetic map; High-throughput SNP genotyping; BARCSoySNP6K; Recombinant inbred line ID THROUGHPUT SNP DISCOVERY; GENOME SEQUENCE; PI 567301B; RESISTANCE; WILD AB The objective of this study was to construct a high-density genetic map of soybean (Glycine max L. Merr) using a high-throughput single nucleotide polymorphism (SNP) genotyping on 357 F-7 recombinant inbred lines from a cross of 'Wyandot' 9 PI 567301B. Of 5,403 SNP loci scored from the Infinium BARCSoySNP6K BeadChip array, 2,585 (48 %) were polymorphic between the two parents and subsequently 2,563 SNPs remained after filtering for minor allele frequency, heterozygosity, and missing data. A total of 2,545 SNPs were mapped into 20 linkage groups corresponding to the 20 chromosomes of soybean. The total length of the map was 2,346 cM with 2,213 unique SNP loci with 86 to 162 unique loci per chromosome. Average marker interval ranged from 0.9 to 1.3 cM with an overall mean of 1.1 cM, but 22 marker intervals were still greater than 10 cM. Colinear relationship was observed between genetic (cM) and physical positions (Mb) of SNPs for most of the genome, highlighting the improvements in the Electronic supplementary material The online version of updated soybean genome assembly Glyma. Wm82.a2 din comparison with the previous Glyma. Wm82.a1. This high-density genetic map shows the utility of the BARCSoySNP6K BeadChip array and will lead to a better understanding of the genetic architecture of complex traits and identification of SNPs tightly linked to QTL for many important soybean traits. C1 [Lee, Sungwoo; Jun, Tae-Hwan; Michel, Andrew P.] Ohio State Univ, Dept Entomol, Wooster, OH 44691 USA. [Freewalt, Keith R.; Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [McHale, Leah K.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. [Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Dorrance, Anne E.] Ohio State Univ, Dept Plant Pathol, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Corn Soybean Wheat Qual Res Unit, Wooster, OH 44691 USA. RP Mian, MAR (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Corn Soybean Wheat Qual Res Unit, Wooster, OH 44691 USA. EM lee.3546@osu.edu; freewalt.6@osu.edu; mchale.21@osu.edu; qijian.song@ars.usda.gov; thjun76@pusan.ac.kr; michel.70@osu.edu; dorrance.1@osu.edu; Rouf.Mian@ars.usda.gov RI McHale, Leah/D-9612-2012 FU United States Department of Agriculture-Agricultural Research Service; Ohio State University/Ohio Agricultural Research and Development Center; Ohio Soybean Council FX This study was funded by United States Department of Agriculture-Agricultural Research Service, the Ohio State University/Ohio Agricultural Research and Development Center, and Ohio Soybean Council. The authors thank Tim Mendiola for his help in developing the mapping population. For SNP genotyping, we acknowledge Vanessa Rashbrook at DNA Technologies, University of California, Davis. NR 25 TC 6 Z9 7 U1 0 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD FEB PY 2015 VL 35 IS 2 AR 58 DI 10.1007/s11032-015-0209-5 PG 7 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA CB6VR UT WOS:000349765300002 ER PT J AU Schlautman, B Fajardo, D Bougie, T Wiesman, E Polashock, J Vorsa, N Steffan, S Zalapa, J AF Schlautman, Brandon Fajardo, Diego Bougie, Tierney Wiesman, Eric Polashock, James Vorsa, Nicholi Steffan, Shawn Zalapa, Juan TI Development and Validation of 697 Novel Polymorphic Genomic and EST-SSR Markers in the American Cranberry (Vaccinium macrocarpon Ait.) SO MOLECULES LA English DT Article ID FIMBRIATED ESCHERICHIA-COLI; MICROSATELLITE MARKERS; LOCI; ADHERENCE AB The American cranberry, Vaccinium macrocarpon Ait., is an economically important North American fruit crop that is consumed because of its unique flavor and potential health benefits. However, a lack of abundant, genome-wide molecular markers has limited the adoption of modern molecular assisted selection approaches in cranberry breeding programs. To increase the number of available markers in the species, this study identified, tested, and validated microsatellite markers from existing nuclear and transcriptome sequencing data. In total, new primers were designed, synthesized, and tested for 979 SSR loci; 697 of the markers amplified allele patterns consistent with single locus segregation in a diploid organism and were considered polymorphic. Of the 697 polymorphic loci, 507 were selected for additional genetic diversity and segregation analyses in 29 cranberry genotypes. More than 95% of the 507 loci did not display segregation distortion at the p < 0.05 level, and contained moderate to high levels of polymorphism with a polymorphic information content >0.25. This comprehensive collection of developed and validated microsatellite loci represents a substantial addition to the molecular tools available for geneticists, genomicists, and breeders in cranberry and Vaccinium. C1 [Schlautman, Brandon; Bougie, Tierney; Zalapa, Juan] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Fajardo, Diego] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Wiesman, Eric; Steffan, Shawn; Zalapa, Juan] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Polashock, James] Rutgers State Univ, USDA ARS, Genet Improvement Fruits & Vegetables Lab, Chatsworth, NJ 08019 USA. [Vorsa, Nicholi] Rutgers State Univ, Blueberry & Cranberry Res & Extens Ctr, Chatsworth, NJ 08019 USA. RP Schlautman, B (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM schlautman@wisc.edu; dfajardo@ncgr.org; tcbougie@wisc.edu; eric.wiesman@ars.usda.gov; james.polashock@ars.usda.gov; vorsa@aesop.rutgers.edu; shawn.steffan@ars.usda.gov; jezalapa@wisc.edu FU National Science Foundation [DBI-1228280]; Wisconsin State Cranberry Growers Association; Cranberry Institute; WI-DATCP (SCBG) [14-002]; USDA-ARS [3655-21220-001-00] FX Rom. 8:31-39. This research was supported by funds from the National Science Foundation (DBI-1228280), Wisconsin State Cranberry Growers Association, Cranberry Institute, WI-DATCP (SCBG Project #14-002), and USDA-ARS (project no. 3655-21220-001-00) provided to J.E.Z. and S.A.S. NR 30 TC 7 Z9 7 U1 0 U2 22 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD FEB PY 2015 VL 20 IS 2 BP 2001 EP 2013 DI 10.3390/molecules20022001 PG 13 WC Chemistry, Organic SC Chemistry GA CD0GH UT WOS:000350748200014 PM 25633331 ER PT J AU Xu, P Stevens, ED French, AD Kovac, P AF Xu, Peng Stevens, Edwin D. French, Alfred D. Kovac, Pavol TI Synthesis and Molecular Structure of the 5-Methoxycarbonylpentyl alpha-Glycoside of the Upstream, Terminal Moiety of the O-Specific Polysaccharide of Vibrio cholerae O1, Serotype Inaba SO MOLECULES LA English DT Article ID CRYSTAL-STRUCTURE; THERMODYNAMIC CONTROL; OGAWA; HEXASACCHARIDE; ANTIGEN; UNIT; O-1; O/1 AB The trimethylsilyl trifluoromethanesulfonate (TMSOTf)-catalyzed reaction of methyl 6-hydroxyhexanoate with 3-O-benzyl-4-(2,4-di-O-acetyl-3-deoxy-l-glycero-tetronamido)-4,6-dideoxy-2-O-levulinoyl-alpha-d-mannopyranosyl trichloroacetimidate followed by a two-step deprotection (hydrogenolysis over Pd/C catalyst and Zemplen deacylation, to simultaneously remove the acetyl and levulinoyl groups) gave 5-(methoxycarbonyl)pentyl 4-(3-deoxy-l-glycero-tetronamido)-4,6-dideoxy-alpha-d-mannopyranoside. The structure of the latter, for which crystals were obtained in the analytically pure state for the first time, followed from its NMR and high-resolution mass spectra and was confirmed by X-ray crystallography. The molecule has two approximately linear components; a line through the aglycon intersects a line through the mannosyl and tetronylamido groups at 120 degrees. The crystal packing separates the aglycon groups from the tetronylamido and mannosyl groups, with only C-H horizontal ellipsis O hydrogen bonding among the aglycon groups and N-H horizontal ellipsis O, O-H horizontal ellipsis O and C-H horizontal ellipsis O links among the tetronylamido and mannosyl groups. A carbonyl oxygen atom accepts the strongest O-H horizontal ellipsis O hydrogen bond and two strong C-H horizontal ellipsis O hydrogen bonds. The geometric properties were compared with those of related molecules. C1 [Xu, Peng; Kovac, Pavol] NIDDK, LBC, NIH, Bethesda, MD 20892 USA. [Stevens, Edwin D.] Western Kentucky Univ, Dept Chem, Bowling Green, KY 42101 USA. [French, Alfred D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Kovac, P (reprint author), NIDDK, LBC, NIH, Bethesda, MD 20892 USA. EM xup3@mail.nih.gov; Edwin.Stevens@wku.edu; Al.French@ars.usda.gov; kpn@helix.nih.gov FU Intramural Research Program of the National Institutes of Health (NIH); National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) FX This research was supported by the Intramural Research Program of the National Institutes of Health (NIH) and National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK). The crystallography was carried out at the University of New Orleans. NR 18 TC 0 Z9 0 U1 2 U2 3 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1420-3049 J9 MOLECULES JI Molecules PD FEB PY 2015 VL 20 IS 2 BP 2892 EP 2902 DI 10.3390/molecules20022892 PG 11 WC Chemistry, Organic SC Chemistry GA CD0GH UT WOS:000350748200066 PM 25679049 ER PT J AU Zhang, Q Eicher, SD Applegate, TJ AF Zhang, Qian Eicher, Susan D. Applegate, Todd J. TI Development of intestinal mucin 2, IgA, and polymeric Ig receptor expressions in broiler chickens and Pekin ducks SO POULTRY SCIENCE LA English DT Article DE broiler chicken; Pekin duck; mucin 2; IgA; polymeric Ig receptor ID GROWTH-FACTOR (IGF)-I; HEAVY-CHAIN LOCUS; IMMUNOGLOBULIN RECEPTOR; MUCOSAL IMMUNITY; GOBLET CELLS; AVIAN EMBRYOS; MATERNAL IGA; REGION GENES; BACTERIA; SYSTEM AB Intestinal mucin 2 (MUC2), a major gel-forming mucin, represents a primary barrier component of mucus layers and a target site for secretory IgA. Polymeric Ig receptor (pIgR) expressed on the basolateral surface of epithelium is used to transport polymeric IgA from the lamina propria into luminal mucins to establish the first lines of intestinal defense. To determine the spatio-temporal expression of MUC2, IgA, and pIgR in broiler chickens and Pekin ducks, intestinal tissues (n = 6/age) were dissected from late embryonic days up to 21 d posthatch. In the intestinal tissues, MUC2 was expressed with a rapid increase at hatching, followed by steady expression through 21 d posthatch both in chickens and ducks. IgA expression was low during the first week following hatching for both species. From the second week posthatch, IgA was rapidly expressed in the chickens, arriving at steady expression in the third week after hatching. However, in ducks, IgA expression during the 2 to 3 wk posthatch period was relatively slow. The expression of pIgR was greatly increased after hatching for both species, but its expression in ducks was relatively delayed. In addition, intestinal pIgR expression was highly correlated with MUC2 and IgA expressions in chickens but just moderately correlated in ducks. The relatively slow and late expression of IgA and pIgR as well as their moderate correlation may or may not account for the susceptibility of ducklings to mucosal pathogens at a young age. C1 [Zhang, Qian; Eicher, Susan D.; Applegate, Todd J.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47906 USA. [Eicher, Susan D.] USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47906 USA. RP Applegate, TJ (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47906 USA. EM applegt@purdue.edu NR 39 TC 2 Z9 2 U1 0 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD FEB PY 2015 VL 94 IS 2 BP 172 EP 180 DI 10.3382/ps/peu064 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA CB7JW UT WOS:000349803800005 PM 25589081 ER PT J AU Ranney, KJ Niemann, JD Lehman, BM Green, TR Jones, AS AF Ranney, Kayla J. Niemann, Jeffrey D. Lehman, Brandon M. Green, Timothy R. Jones, Andrew S. TI A method to downscale soil moisture to fine resolutions using topographic, vegetation, and soil data SO ADVANCES IN WATER RESOURCES LA English DT Article DE Catchment; Canopy; Litter; Soil texture; EMT; EOF ID HYDROLOGICAL FLUX MEASUREMENTS; MAIZE-CROPPED FIELD; LEAF-AREA INDEX; SPATIAL VARIABILITY; DATA SET; PATTERNS; SCALE; EVAPORATION; WATER; TERRAIN AB Soil moisture can be estimated over large regions with spatial resolutions greater than 500 m, but many applications require finer resolutions (10-100 m). Several methods use topographic data to downscale, but vegetation and soil patterns can also be important. In this paper, a downscaling model that uses fine- resolution topographic, vegetation, and soil data is presented. The method is tested at the Cache la Poudre catchment where detailed vegetation and soil data were collected. Additional testing is performed at the Tarrawarra and Nerrigundah catchments where limited soil data are available. Downscaled soil moisture patterns at Cache la Poudre improve when vegetation and soil data are used, and model performance is similar to an EOF method. Using interpolated soil data at Tarrawarra and Nerrigundah decreases model performance and results in worse performance than an EOF method, suggesting that soil data needs greater spatial detail and accuracy to be useful for downscaling. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Ranney, Kayla J.; Niemann, Jeffrey D.; Lehman, Brandon M.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Green, Timothy R.] USDA ARS, Ft Collins, CO 80526 USA. [Jones, Andrew S.] Colorado State Univ, Cooperat Inst Res Atmosphere, Ft Collins, CO 80523 USA. RP Niemann, JD (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Campus Delivery 1372, Ft Collins, CO 80523 USA. EM jniemann@engr.colostate.edu RI Jones, Andrew/D-3291-2012 OI Jones, Andrew/0000-0002-0995-4957 FU Army Research Office Terrestrial Sciences Program [W911NF-11-1-0438] FX We would like to thank Stephanie Kampf for her insightful comments, Kevin Werbylo and Mike Coleman for their technical support, the Army Research Office Terrestrial Sciences Program (W911NF-11-1-0438) for their financial support, and two anonymous reviewers for their helpful suggestions. NR 59 TC 4 Z9 4 U1 3 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD FEB PY 2015 VL 76 BP 81 EP 96 DI 10.1016/j.advwatres.2014.12.003 PG 16 WC Water Resources SC Water Resources GA CB5NE UT WOS:000349673500007 ER PT J AU D'Aiuto, PE Patt, JM Albano, JP Shatters, RG Evens, TJ AF D'Aiuto, P. E. Patt, J. M. Albano, J. P. Shatters, R. G. Evens, T. J. TI Algal turf scrubbers: Periphyton production and nutrient recovery on a South Florida citrus farm SO ECOLOGICAL ENGINEERING LA English DT Article DE ATS; Algal turf scrubber; Periphyton; Phosphorus removal; Nitrogen removal ID FRESH-WATER ALGAE; ELEMENTAL COMPOSITION; MANAGEMENT-PRACTICES; LOADING RATES; BIOMASS; ECOSYSTEMS; CHEMISTRY; COMMUNITY; AMMONIUM AB There is a strong need to develop strategies that reduce nutrient loading to Florida's waters. The purpose of this study was to investigate the nutrient-removing ability and growth rate of periphyton, grown on an Algal Turf Scrubber (ATS (TM)) that received runoff from a citrus orchard operated by the USDA in southern Florida. A pilot scale ATS (1.2 m wide x 234 m long; 0.5% declining grade) was constructed and received a continuous flow (227 L min (1)) of water pumped from the orchard's drainage canal. Over an 18-month period, PO4-P,NO3-N,NO2-N and NH4+ removal averaged 16%, 49%, 19% and 41%, respectively. On average, the entire flow-way yielded 5.5 gm (2) day (1) (range: 1-16 gm (2) day (1)) of periphyton (dry weight). However, the upper 60 m yielded 11 gm (2) day (1) (range: 1-26 gm (2) day (1)). Over 54% of total production occurred in the upper 26% of the flow-way and growth rate over the entire flow-way, increased 195% during the summer months (June-Aug.) when compared to winter months (Jan.-Mar.). Harvested periphyton contained an average of 24.1% C, 3.8% N, 0.38% P, and 0.003% Si. The harvesting of periphyton from the entire flow-way removed a mean of 0.02 gm (2) day (1) of total phosphorus, and 0.18 gm (2) day (1) of total nitrogen. However, the highly productive upstream (1-30 m) section of the flow-way removed a mean of 0.05 gm (2) day (1) of total phosphorus, and 0.49 gm (2) day (1) of total nitrogen. During colonization, diatom chains (Aulocoseira spp.) along with various pennate diatoms dominated. However, the community was dominated by filamentous Chlorophyta for most of the study. Although this is a preliminary study to provide foundational information necessary for future optimization experiments, nutrient removal observed suggests a potential for the use of ATSs as an effective means of treating agricultural runoff from citrus and other fruit tree orchards while yielding periphyton. Published by Elsevier B.V. C1 [D'Aiuto, P. E.; Patt, J. M.; Albano, J. P.; Shatters, R. G.; Evens, T. J.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP D'Aiuto, PE (reprint author), USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM Peter.Daiuto@ars.usda.gov; Joseph.Patt@ars.usda.gov; Joseph.Albano@ars.usda.gov; Robert.Shatters@ars.usda.gov FU USDA - ARS FX This paper is dedicated to the memory of our colleague and friend T.J. Evens, who initiated this study. The authors thank C. Lasser, R. Alessandro, R. Hawley, C. Orcutt, M. Wikarek, S. Mayo, L. Myers, and K. Hovey for their excellent technical assistance. Hydromentia Inc., provided invaluable technical advice. Dr. J.C. Makarewicz, N.Y. State University at Brockport, M. S. Wood, USGS and S. Wellman, Pellissippi State Community College offered excellent editing advice. We also thank K. Manoylov, Georgia State University, for her assistance with algal identifications. The algal turf scrubber is a patented water treatment technology developed by W. Adey and held by the Smithsonion Institution. This study was funded by the USDA - ARS. Mention of trade names or commercial products is solely for providing specific information and does not imply recommendation or endorsement by the USDA. NR 28 TC 7 Z9 7 U1 3 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD FEB PY 2015 VL 75 BP 404 EP 412 DI 10.1016/j.ecoleng.2014.11.054 PG 9 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA CB4LC UT WOS:000349598200049 ER PT J AU Moorman, TB Tomer, MD Smith, DR Jaynes, DB AF Moorman, Thomas B. Tomer, Mark D. Smith, Douglas R. Jaynes, Dan B. TI Evaluating the potential role of denitrifying bioreactors in reducing watershed-scale nitrate loads: A case study comparing three Midwestern (USA) watersheds SO ECOLOGICAL ENGINEERING LA English DT Article DE Denitrification; Nitrate; Tile-drainage; Wood-chip bioreactor; Watershed ID DRAINAGE DENITRIFICATION BIOREACTOR; GULF-OF-MEXICO; SUBSURFACE DRAINAGE; AGRICULTURAL WATERSHEDS; MISSISSIPPI RIVER; STREAMFLOW; IOWA; PERFORMANCE; STRATEGIES; ILLINOIS AB The transport of nitrate (NO3-N) from agricultural lands to surface waters is a complex and recalcitrant problem. Subsurface drainage systems that are especially prevalent in the corn-growing regions of the Midwestern USA facilitate NO3-N transport. Several conservation practices, including fertilizer and manure management, cover crops, natural and installed wetlands, and wood-chip denitrification bioreactors are options that can mitigate NO3-N losses from agricultural lands. Using simple methods of estimation we examine the cumulative volume of denitrification bioreactors required to treat various amounts of NO3-N in base flow, a proxy for tile drainage, at the watershed scale. The use of load duration curves from three different watersheds shows that NO3-N transport is disproportionately skewed toward larger daily base flows. Approximately 50% of the annual NO3-N is transported in largest 30% of daily base flows. Using previous estimates of NO3-N removal by wood-chip bioreactors, we calculated cumulative bioreactor volumes needed to achieve a range of hydraulic residence times (HRT) given rates of base flow observed in three agricultural watersheds. These analyses suggest that cumulative watershed bioreactor volumes sufficient to achieve an HRT of 0.5 days will reduce at least 20% of the total annual NO3-N loss in one watershed and 30% in the other two watersheds. The area required for wood-chip bioreactors would be at most 0.27% of the watershed area. Published by Elsevier B.V. C1 [Moorman, Thomas B.; Tomer, Mark D.; Jaynes, Dan B.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Smith, Douglas R.] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Moorman, TB (reprint author), ARS, USDA, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM tom.moorman@ars.usda.gov NR 40 TC 7 Z9 7 U1 6 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD FEB PY 2015 VL 75 BP 441 EP 448 DI 10.1016/j.ecoleng.2014.11.062 PG 8 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA CB4LC UT WOS:000349598200053 ER PT J AU Ouyang, Y Leininger, TD Moran, M AF Ouyang, Ying Leininger, Theodor D. Moran, Matt TI Estimating effects of reforestation on nitrogen and phosphorus load reductions in the Lower Yazoo River Watershed, Mississippi SO ECOLOGICAL ENGINEERING LA English DT Article DE HSPF model; Nitrate and orthophosphate load; Reforestation; Yazoo River Watershed ID GULF-OF-MEXICO; HYPOXIA; BASIN; BASEFLOW; SCIENCE; FLUX; USA AB Surface water quality in the Lower Mississippi River Basin (LMRB) and the adjacent Gulf of Mexico has degraded over the past several decades primarily due to deforestation to agricultural lands and the loss of wetlands. This study investigated the benefits of reforestation upon nitrate-nitrogen (NO3-- - -NN) and orthophosphate (PO43-) load reductions in the Lower Yazoo River Watershed (LYRW) within the LMRB using the BASINS-HSPF model. The model was calibrated and validated with available experimental data prior to its applications. Two simulation scenarios were then performed: one was chosen to predict the NO3-- - -N and PO43- loads without reforestation and the other was selected to estimate the impacts of reforestation upon NO3-- - -N and PO43- load reductions following the conversion of 25, 50, 75, and 100% of the agricultural lands (with most lands near or in the batture of the streams) into forests. In general, an increase in forests reduced NO3-- - -N and PO43- loads and occurred because forest soils enriched in organic matter absorb water and nutrients and reduce the surface water runoff. Overall, a two-fold increase in forest land would result in approximately two-fold decrease in annual average NO3-- - -N and PO43- loads. On average, over a 10-year simulation, the specific NO3-- - -N and PO43- load reductions were, respectively, 0.06 and 0.004 ton/ha/y. Although the annual average NO3-- - -N and PO43- loads always decreased with increasing forest land conversion, the optimal specific NO3-- - -N and PO43- load reductions were found at a 75% reduction of agricultural land for the simulation conditions used in this study. Additionally, the annual average NO3-- - -N load was about 16 times higher than that of PO43- in the LYRW. This study suggests that reforestation in or around the batture of streams is a beneficial practice for NO3-- - -N and PO43- load reductions. Published by Elsevier B.V. C1 [Ouyang, Ying; Moran, Matt] US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Mississippi State, MS 39762 USA. [Leininger, Theodor D.] US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. RP Ouyang, Y (reprint author), US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, 100 Stone Blvd,Thompson Hall,Room 309, Mississippi State, MS 39762 USA. EM youyang@fs.fed.us FU US Endowment for Forestry and Communities (Endowment), Greenville, SC FX The study was supported by US Endowment for Forestry and Communities (Endowment), Greenville, SC. The authors thank Mr. Peter Stangel from the Endowment for his valuable comments and suggestions. NR 27 TC 0 Z9 0 U1 6 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD FEB PY 2015 VL 75 BP 449 EP 456 DI 10.1016/j.ecoleng.2014.11.032 PG 8 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA CB4LC UT WOS:000349598200054 ER PT J AU Barnes-Mauthe, M Gray, SA Arita, S Lynham, J Leung, PS AF Barnes-Mauthe, Michele Gray, Steven Allen Arita, Shawn Lynham, John Leung, PingSun TI What Determines Social Capital in a Social-Ecological System? Insights from a Network Perspective SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Social networks; Social capital; Human capital; Ethnic diversity; Stakeholder attributes; Natural resource management ID NATURAL-RESOURCE MANAGEMENT; WEAK TIES; FISHERIES MANAGEMENT; ECONOMIC-ACTION; CENTRALITY; INFORMATION; HAWAII; EMBEDDEDNESS; IMMIGRATION; PERFORMANCE AB Social capital is an important resource that can be mobilized for purposive action or competitive gain. The distribution of social capital in social-ecological systems can determine who is more productive at extracting ecological resources and who emerges as influential in guiding their management, thereby empowering some while disempowering others. Despite its importance, the factors that contribute to variation in social capital among individuals have not been widely studied. We adopt a network perspective to examine what determines social capital among individuals in social-ecological systems. We begin by identifying network measures of social capital relevant for individuals in this context, and review existing evidence concerning their determinants. Using a complete social network dataset from Hawaii's longline fishery, we employ social network analysis and other statistical methods to empirically estimate these measures and determine the extent to which individual stakeholder attributes explain variation within them. We find that ethnicity is the strongest predictor of social capital. Measures of human capital (i.e., education, experience), years living in the community, and information-sharing attitudes are also important. Surprisingly, we find that when controlling for other factors, industry leaders and formal fishery representatives are generally not well connected. Our results offer new quantitative insights on the relationship between stakeholder diversity, social networks, and social capital in a coupled social-ecological system, which can aid in identifying barriers and opportunities for action to overcome resource management problems. Our results also have implications for achieving resource governance that is not only ecologically and economically sustainable, but also equitable. C1 [Barnes-Mauthe, Michele; Leung, PingSun] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Barnes-Mauthe, Michele] Univ Hawaii Manoa, Joint Inst Marine & Atmospher Res, Honolulu, HI 96822 USA. [Gray, Steven Allen] Univ Massachusetts, Boston Sch Environm, Boston, MA 02125 USA. [Arita, Shawn] USDA, Econ Res Serv, Washington, DC 20024 USA. [Lynham, John] Univ Hawaii Manoa, Dept Econ, Honolulu, HI 96822 USA. RP Barnes-Mauthe, M (reprint author), Univ Hawaii Manoa, Dept Nat Resources & Environm Management, 1910 East West Rd, Honolulu, HI 96822 USA. EM barnesm@hawaii.edu FU U.S. National Science Foundation [GEO-1211972]; Pelagic Fisheries Research Program FX Funding for this research was provided by the U.S. National Science Foundation Grant #GEO-1211972 and the Pelagic Fisheries Research Program. We are grateful to the participants of the Networks and Natural Resource Management session at the International Network for Social Network Analysis Sunbelt 2014 meeting, and particularly Orjan Bodin, Christina Prell, and Steve Borgatti, who provided invaluable feedback on this work. We thank all fishers who have been involved in this ongoing project, Joey Lecky for his graphic design expertise, Minling Pan of the NOAA Pacific Islands Fisheries Science Center for her support, and three anonymous reviewers for their thoughtful and constructive comments. NR 128 TC 4 Z9 4 U1 7 U2 52 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD FEB PY 2015 VL 55 IS 2 BP 392 EP 410 DI 10.1007/s00267-014-0395-7 PG 19 WC Environmental Sciences SC Environmental Sciences & Ecology GA CB3UI UT WOS:000349554100011 PM 25376745 ER PT J AU Westfall, JA AF Westfall, James A. TI Selection of Forest Inventory Cycle Length Based on Growth Rate and Measurement Variability SO FOREST SCIENCE LA English DT Article DE error propagation; signal-to-noise ratio; tree biomass; data quality ID STATISTICAL POWER AB As pressures on forest resources mount, there is an increasing need for up-to-date information on which sound management and policy decisions can be made. This is evident in the adoption of shorter inventory cycle lengths for national forest inventories in the past few decades. While these intensified efforts are meritorious, substantial efficiency can be lost if cycle lengths are too short in relation to growth in the population. Specifically, if growth is small in relation to measurement variation, the signal-to-noise ratio is small and considerable expense is incurred to obtain data of limited usefulness. To address this issue, the relationships between measurement variation and growth rates were examined across a spatial gradient in the eastern United States. As tree measurements are often not used directly, these relationships were also analyzed in the context of tree biomass prediction models of varying complexity. The results indicated that for latitude range N 30-38 degrees, where many fast-growing plantations are found, cycle lengths as short as 3 years may be acceptable if the biomass model only relies on tree diameter; however, increased cycle lengths should be chosen for more northerly latitudes. Longer cycle lengths are also suggested when employing more complex biomass models requiring additional inputs such as tree height and crown ratio, which are subject to higher levels of measurement variability. The methods provide a framework for determining cycle lengths that may considerably improve the efficiency and effectiveness of ongoing forest inventory and monitoring efforts. C1 US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. RP Westfall, JA (reprint author), US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. EM jameswestfall@fs.fed.us NR 18 TC 0 Z9 0 U1 0 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 17 EP 24 DI 10.5849/forsci.13-202 PG 8 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100002 ER PT J AU Hepp, TE Vimmerstedt, JP Smalley, GW McNab, WH AF Hepp, Todd E. Vimmerstedt, John P. Smalley, Glendon W. McNab, W. Henry TI Estimating Yields of Unthinned Eastern White Pine Plantations from Current Stocking in the Southern Appalachians SO FOREST SCIENCE LA English DT Article DE basal area; board foot volume; cubic volume; Pinus strobus; site quality; survival ID HEIGHT-GROWTH; UNITED-STATES AB Eastern white pine (Pious strobus L.) is a highly productive native conifer of the southern Appalachian Mountains that has long been established in plantations for conventional purposes of afforestation and timber production and potentially for carbon sequestration both within and outside its natural range. Growth-and-yield models are not available, however, for use by land managers to evaluate potential economic value of plantations established on sites of various qualities over time. Data from 78 plantations in the southern Appalachian Mountains of Georgia, North Carolina, and Virginia were used to develop models for estimation of survival, basal area, and yields of cubic and board feet as functions of stand age, site quality, and stocking. Stand structure and volume yields were strongly related to stand age and site quality. Compared to plantations on sites of lower quality, stands on good sites had lower survival but higher basal area stocking, cubic volume, and sawtimber yields. C1 [Hepp, Todd E.] Tennessee Valley Author, Knoxville, TN USA. [Vimmerstedt, John P.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Smalley, Glendon W.] US Forest Serv, USDA, Southern Res Stn, Sewanee, TN USA. [McNab, W. Henry] US Forest Serv, USDA, Southern Res Stn, Asheville, NC USA. EM hmcnab@fs.fed.us NR 28 TC 0 Z9 0 U1 1 U2 4 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 114 EP 122 DI 10.5849/forsci.13-620 PG 9 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100012 ER PT J AU Zhou, XH Schoeneberger, MM Brandle, JR Awada, TN Chu, JM Martin, DL Li, JH Li, YQ Mize, CW AF Zhou, Xinhua Schoeneberger, Michele M. Brandle, James R. Awada, Tala N. Chu, Jianmin Martin, Derrel L. Li, Jihong Li, Yuqiang Mize, Carl W. TI Analyzing the Uncertainties in Use of Forest-Derived Biomass Equations for Open-Grown Trees in Agricultural Land SO FOREST SCIENCE LA English DT Article DE agroforestry; Austrian pine; carbon sequestration; eastern redcedar; green ash; windbreak ID CARBON SEQUESTRATION; UNITED-STATES; AGROFORESTRY; SHELTERBELT; GRAVITY; PINE; WOOD AB Quantifying carbon in agroforestry trees requires biomass equations that capture the growth differences (e.g., tree specific gravity and architecture) created in the more open canopies of agroforestry plantings compared with those generally encountered in forests. Whereas forest-derived equations are available, equations for open-grown trees are not. Data from destructively sampled open-grown trees in the Northern Great Plains were used to examine the uncertainties in the use of forest-derived equations for open-grown trees. Three species, representative of major morphological types of agroforestry trees, were studied: green ash, Austrian pine, and eastern redcedar. Forest-derived equations provided good estimates of trunk biomass at lower diameter ranges but, as diameter increased, resulted in overestimation up to 40% for individual trees. Across the full diameter ranges, individual tree branch biomass was underestimated by 29-82%, depending on species and equation source (regional or nonregional). Although open-grown trunk and branch biomass curves diverged down and up, respectively, from their forest-derived counterparts, those for the whole tree tended to converge, albeit significantly above the forest-derived curves. Whole-tree biomass for individual trees was underestimated by at least 18%. To correct the biases, we studied the adjustment factor of forest- to open-grown tree biomass. It shows a power function with diameter. On a whole-tree basis, it was evaluated as a constant (1.2) independent of species and diameters. Application of this constant factor adjusted the biomass underestimation of three-species-mixed plantation by forest-derived equations from 2110 4.6%, providing a cost-efficient approach to use forest-derived equations for open-grown trees in agriculture land. C1 [Zhou, Xinhua; Brandle, James R.; Awada, Tala N.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Schoeneberger, Michele M.] US Forest Serv, USDA, Natl Agroforestry Ctr, Eastern Ctr, CA USA. [Chu, Jianmin] Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing, Peoples R China. [Martin, Derrel L.] Univ Nebraska, Dept Biol Syst Engn, Lincoln, NE 68583 USA. [Li, Jihong] Northeast Forestry Univ, Sch Forestry, Dept Forest Management, Harbin, Heilongjiang, Peoples R China. [Li, Yuqiang] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Beijing 100864, Peoples R China. RP Zhou, XH (reprint author), Campbell Sci Inc, Logan, UT 84321 USA. EM xzhou@campbellsci.com; mschoeneberger@fs.fed.us; jbrandle1@unl.edu; tawada2@unl.edu; cjmcaf@163.com; dmartin2@unl.edu; lee-jihong@126.com; liyq@lzb.ac.cn; carlmize@gmail.com FU USDA Forest Service [10-JV-11330152-045]; USDA Forest Service, Southern Research Station, the McIntyre-Stennis Forestry Research Program; USDA/Cooperative State Research Service National Research Initiative [2001-35108-10205]; National Natural Science Foundation of China [31370707]; Montana Watershed, Inc. FX This research was supported in part by funds provided through the USDA Forest Service (Agreement 10-JV-11330152-045), the USDA Forest Service, Southern Research Station, the McIntyre-Stennis Forestry Research Program, the USDA/Cooperative State Research Service National Research Initiative (Competitive Grant 2001-35108-10205), the National Natural Science Foundation of China (31370707), and Montana Watershed, Inc. We appreciate the field help of K.A. and C.L. Messenger and personnel in the Montana and Nebraska Natural Resource Conservation Service field offices as well as the editorial work by the supporting staff of R. Carman and N. Hammond at the USDA National Agroforestry Center. We thank the associate editor and the three anonymous reviewers for their helpful comments. NR 56 TC 1 Z9 1 U1 2 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 144 EP 161 DI 10.5849/forsci.13-071 PG 18 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100016 ER PT J AU Sprinkle, W Klepac, J AF Sprinkle, Wesley Klepac, John TI Models of Utah Juniper and Two-Needle Pinyon Biomass for One Site in Southwest Utah SO FOREST SCIENCE LA English DT Article DE biomass; regression; juniper; pinyon; modeling ID UNITED-STATES; TREE; PRODUCTIVITY; VEGETATION; EQUATIONS; ARIZONA; MASS AB Twenty-three Utah juniper (Juniperus osteosperma) trees and 20 two-needle pinyon pine (Pinus edulis) trees in southwest Utah were selected, and whole-tree green weights were obtained. In addition, trees were measured for diameter at groundline (dgl), dbh, crown length and width, and total height. These data were used to model aboveground biomass for both species. The coefficient of determination (R-2), standard error of the estimate, mean percent standard error, and percent bias were calculated for each model. For both species, the best model for estimating aboveground biomass included dgl, crown width, and total height as predictors. With these predictors, the model for Utah juniper had R-2 = 0.97, whereas the model for pinyon pine had R-2 = 0.98. Both dgl and dbh alone were also very good predictors of aboveground biomass and had R-2 values for Utah juniper of 0.94 and 0.95, respectively. For pinyon pine, using dgl and dbh as predictors, R-2 values were 0.98 and 0.97, respectively. Site-specific models outperformed published biomass equations. C1 [Sprinkle, Wesley] US Forest Serv, USDA, Washington, DC 20252 USA. [Klepac, John] US Forest Serv, USDA, GW Andrews Forestry Sci Lab, Auburn, AL USA. RP Sprinkle, W (reprint author), US Forest Serv, USDA, Washington, DC 20252 USA. EM wesleysprinkle@gmail.com; jklepac@fs.fed.us NR 34 TC 0 Z9 0 U1 1 U2 4 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 162 EP 168 DI 10.5849/forsci.12-541 PG 7 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100017 ER PT J AU Zapata-Valenzuela, JA Ogut, F Kegley, A Cumbie, P Isik, F Li, BL McKeand, SE AF Zapata-Valenzuela, Jaime A. Ogut, Funda Kegley, Angela Cumbie, Patrick Isik, Fikret Li, Bailian McKeand, Steven E. TI Seedling Evaluation of Atlantic Coastal and Piedmont Sources of Pinus taeda L. and Their Hybrids for Cold Hardiness SO FOREST SCIENCE LA English DT Article DE freezing test; cold tolerance; genetic correlation ID LOBLOLLY-PINE; ELECTROLYTE LEAKAGE; FREEZING TOLERANCE; PROVENANCES; ACCLIMATION; SYLVESTRIS; FAMILIES; TREES AB Seedlings of 59 loblolly pine (Pinus taeda L.) polycross families representing four populations (Atlantic Coastal, Piedmont, Coastal x Piedmont, and Piedmont x Coastal) were evaluated for cold hardiness. Seedlings were preconditioned in phytotron environments and then subjected to a freezing treatment. Freezing injury, mortality, and size of seedlings were assessed. Significant differences in cold injury were found among populations and families within each population. The Piedmont families and hybrid seedlings with a Piedmont maternal parent had less injury and higher survival relative to those of the other populations. Most families with high cold injury had a Coastal maternal parent, whereas most families with the least cold injury had a Piedmont maternal parent. The large observed among- and within-population variations in cold injury suggested that family or individual-within-family selection could improve cold hardiness in loblolly pine. Based on the results of seedling growth and cold injury, there may be an advantage to deploying Piedmont x Coastal hybrids or Piedmont families on more adverse sites, e.g., cold sites. On the milder sites, deployment of Coastal x Piedmont hybrids or hardy Coastal families seems appropriate. C1 [Zapata-Valenzuela, Jaime A.; Ogut, Funda; Li, Bailian] N Carolina State Univ, Coronel, Chile. [Zapata-Valenzuela, Jaime A.] Bioforest SA, Coronel, Chile. [Kegley, Angela] US Forest Serv, USDA, Washington, DC USA. [Cumbie, Patrick] ArborGen Inc, Summerville, SC USA. [Isik, Fikret; McKeand, Steven E.] N Carolina State Univ, Dept Forestry & Environm Resources, Coronel, Chile. RP Zapata-Valenzuela, JA (reprint author), N Carolina State Univ, Coronel, Chile. EM jzapata@arauco.cl FU Cooperative Tree Improvement Program; Department of Forestry and Environmental Resources at North Carolina State University; Southeastern Partnership for Integrated Biomass Supply Systems (IBSS) project; Agriculture and Food Research Initiative Competitive Grant [2011-68005-30410]; Pine Integrated Network: Education, Mitigation and Adaptation Project (PINEMAP), a Coordinated Agricultural Project (CAP) - USDA National Institute of Food and Agriculture [2011-68002-30185] FX This study was conducted with funds and support from the Cooperative Tree Improvement Program and the Department of Forestry and Environmental Resources at North Carolina State University. Additional support came from the Southeastern Partnership for Integrated Biomass Supply Systems (IBSS) project, which is supported by Agriculture and Food Research Initiative Competitive Grant 2011-68005-30410 and from the Pine Integrated Network: Education, Mitigation and Adaptation Project (PINEMAP), a Coordinated Agricultural Project (CAP) funded by the USDA National Institute of Food and Agriculture (Grant 2011-68002-30185). NR 30 TC 1 Z9 1 U1 2 U2 8 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 169 EP 175 DI 10.5849/forsci.12-610 PG 7 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100018 ER PT J AU Bragg, DC Guldin, JM AF Bragg, Don C. Guldin, James M. TI The Silvicultural Implications of Age Patterns in Two Southern Pine Stands after 72 Years of Uneven-Aged Management SO FOREST SCIENCE LA English DT Article DE Crossett Experimental Forest; loblolly; shortleaf; stand structure ID RED-COCKADED WOODPECKERS; UNITED-STATES; FOREST; EXPERIENCE; SELECTION; ARKANSAS AB A randomized sample of 250 loblolly (Pinus taeda L.) and shortleaf (Pinus echinata Mill.) pine ring counts was collected from the Good and Poor Farm Forestry compartments on the Crossett Experimental Forest. These mature, pine-dominated stands have been managed using uneven-aged silviculture since 1937. Our sample shows that both of these compartments have many different age classes although few distinct cohorts. Over the decades, pine recruitment followed the dozens of timber harvests and occasional natural mortality events (e.g., lightning strikes, ice storms, windthrow, insects, and disease). After more than 70 years of active management, only 5% of the overstory pines are shortleaf and about 6% of all pines originated before the imposition of uneven-aged silviculture. The age structure of these stands can be used to adapt. conventional silvicultural treatments. For example, a wide range of ages was found in the sawtimber size classes, indicating that productivity improvements are still possible. The data also suggest that it may be possible to modify current practices to alter the age structure to favor other kinds of ecosystem services (e.g., wildlife habitat). C1 [Bragg, Don C.] US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71655 USA. [Guldin, James M.] US Forest Serv, USDA, Monticello, AR USA. RP Bragg, DC (reprint author), US Forest Serv, USDA, Southern Res Stn, Monticello, AR 71655 USA. EM dbragg@fs.fed.us; jguldin@fs.fed.us NR 40 TC 1 Z9 1 U1 2 U2 14 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 176 EP 182 DI 10.5849/forsci.13-137 PG 7 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100019 ER PT J AU Olson, MG Gwaze, D Elliott, AG AF Olson, Matthew G. Gwaze, David Elliott, Anthony G. TI Fate of Live Trees Retained in Mixed Bottom land Hardwood Stands during the First Decade after Harvest SO FOREST SCIENCE LA English DT Article DE basal area retention; clearcutting; residual stand damage; floodplain forests; aesthetics ID MISSOURI; FORESTS; RETENTION; DAMAGE AB The effects of clearcutting with reserves (CCR), basal area retention (BAR), and no-harvest control (CTL) on residual trees in a bottomland hardwood forest in northeastern Missouri were evaluated during the first decade after harvest. Harvesting damaged 15 and 46% of live trees (>4.5 in. dbh) retained in BAR and (CCR treatments, respectively. Three years after harvest (2000-2003), the annual mortality rate was higher in the CCR (6.8%) than in the BAR (3.2%) or the CTL (1.5%). From 2003 to 2010, the annual mortality rate of the BAR remained the same, whereas mortality rates in the CU declined substantially (2.5%), suggesting a pulse of mortality during the first few years after clearcutting. A thinning effect of harvesting (i.e., growth increase) was observed in both harvest treatments but only during the second time period in the CCR. Basal area growth was greatest in the BAR during the first 3 years, because of low mortality and moderate diameter growth. The results from this study suggest that BAR does not increase mortality of residual trees and improves residual tree growth, including that of desirable oak species. Therefore, BAR is a suitable option for managers interested in managing mixed bottomland hardwoods for wildlife habitat and forest products while potentially mitigating the negative visual impacts of harvesting. C1 [Olson, Matthew G.; Elliott, Anthony G.] Missouri Dept Conservat, West Plains, MO 65775 USA. [Gwaze, David] US Forest Serv, USDA, Washington, DC USA. RP Olson, MG (reprint author), Missouri Dept Conservat, West Plains, MO 65775 USA. EM matthew.olson@mdc.mo.gov; dgwaze@fs.fed.us; anthony.elliott@mdc.mo.gov FU Missouri Department of Conservation FX We are grateful to Brian Root for years of project leadership and to the numerous interns for collecting the data. We thank Sherry Gao for statistical advice. We also thank the Missouri Department of Conservation for funding this study. NR 22 TC 0 Z9 0 U1 1 U2 5 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 190 EP 196 DI 10.5849/forsci.13-730 PG 7 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100021 ER PT J AU Lakel, WA Aust, WM Dolloff, CA Keyser, PD AF Lakel, William A., III Aust, Wallace M. Dolloff, C. Andrew Keyser, Patrick D. TI Residual Timber Values within Piedmont Streamside Management Zones of Different Widths and Harvest Levels SO FOREST SCIENCE LA English DT Article DE best management practices; riparian forests; streamside management zone costs ID WATER-QUALITY; LOBLOLLY-PINE; UNITED-STATES; FOREST MANAGEMENT; ICE DAMAGE; SEDIMENT; GUIDELINES; VIRGINIA; GEORGIA; USA AB Forested streamside management zones (SMZs) provide numerous societal benefits including protection of water quality and enhancement of in-stream and riparian habitats. However, values of residual timber in SMZs are often ignored, yet maintenance of unnecessarily wide SMZs can potentially reduce merchantable timber. Therefore, forestland owners, managers, and logging contractors are interested in determining minimum SMZ widths and stocking levels that can effectively maintain water quality while minimizing residual SMZ stand values. A larger SMZ efficacy study evaluated the efficacy of 7.6-m SMZs with no thinning, 15.2-m SMZs with no thinning, 15.2-m SMZs with thinning, and 30.2-m SMZs without thinning within 16 operational clearcuts. All SMZs widths provided effective sediment control (Lakel et al. 2010). The substudy presented here evaluated residual values in the SMZs of the larger study. Analyses examined immediate values associated with foregoing removal of SMZ timber and provide insight into future SMZ management issues. Across 16 harvested tracts, SMZs accounted for approximately 12% of the total harvest area with an average SMZ residual timber value of $1,064.78/ha. This study supports the financial benefits of partial harvests within the SMZs that remove higher value trees as typically recommended or permitted by best management practice guidelines. However, partial harvests may promote less valuable shade-tolerant species. Results also indicated that almost one-half of the SMZs had notable wind and/or ice damage within 2 years of installation. Financial analysis including opportunities for a tax credit indicate that longer term management of SMZs may be financially feasible if valuable products continue to be thinned from these stands along with rotational harvesting of the adjacent upland stands. Overall, results indicate that society and landowner short-term goals for typical southern Piedmont sites can generally be achieved by leaving 15.2-m SMZs and thinning the stands where practical. C1 [Lakel, William A., III] Virginia Dept Forestry, Charlottesville, VA 22903 USA. [Aust, Wallace M.] Virginia Polytech Inst & State Univ, Blacksburg, VA 24061 USA. [Dolloff, C. Andrew] US Forest Serv, USDA, Washington, DC USA. [Keyser, Patrick D.] Univ Tennessee, Knoxville, TN 37996 USA. RP Lakel, WA (reprint author), Virginia Dept Forestry, Charlottesville, VA 22903 USA. EM wlakel@vt.edu; waust@vt.edu; adolloff@fs.fed.us; pkeyser@utk.edu FU National Council for Air and Stream Improvement, Inc.; USDA Forest Service; MeadWestvaco Corporation; Virginia Department of Forestry; Virginia Tech Department of Forest Resources and Environmental Conservation FX This research was supported by the National Council for Air and Stream Improvement, Inc., the USDA Forest Service, MeadWestvaco Corporation, the Virginia Department of Forestry, and the Virginia Tech Department of Forest Resources and Environmental Conservation. NR 59 TC 0 Z9 0 U1 1 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD FEB PY 2015 VL 61 IS 1 BP 197 EP 204 DI 10.5849/forsci.13-608 PG 8 WC Forestry SC Forestry GA CB4HZ UT WOS:000349590100022 ER PT J AU Islam, N Li, G Garrett, WM Lin, RS Sriram, G Cooper, B Coleman, GD AF Islam, Nazrul Li, Gen Garrett, Wesley M. Lin, Rongshuang Sriram, Ganesh Cooper, Bret Coleman, Gary D. TI Proteomics of Nitrogen Remobilization in Poplar Bark SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE Populus; mass spectrometry; protease storage; MudPET ID NUTRIENT USE EFFICIENCY; POPULUS X CANADENSIS; PROTEIN IDENTIFICATION TECHNOLOGY; CARBOXYPEPTIDASE-LIKE PROTEIN; VACUOLAR SORTING RECEPTOR; LIPID-TRANSFER PROTEINS; MASS-SPECTROMETRY DATA; STORAGE PROTEIN; SERINE-CARBOXYPEPTIDASE; CYSTEINE PROTEASE AB Seasonal nitrogen (N) cycling in temperate deciduous trees involves the accumulation of bark storage proteins (BSPs) in phloem parenchyma and xylem ray cells. BSPs are anabolized using recycled N during autumn leaf senescence and later become a source of N during spring shoot growth as they are catabolized. Little is known about the catabolic processes involved in remobilization and reutilization of N from BSPs in trees. In this study, we used multidimensional protein identification technology (MudPIT) and spectral counting to identify protein changes that occur in the bark during BSP catabolism. A total of 4,178 proteins were identified from bark prior to and during BSP catabolism. The majority (62%) of the proteins were found during BSP catabolism, indicating extensive remodeling of the proteome during renewed shoot growth and N remobilization. Among these proteins were 30 proteases, the relative abundances of which increased during BSP catabolism. These proteases spanned a range of families including members of the papain-like cysteine proteases, serine carboxypeptidases, and aspartyl proteases. These data identify, for the first time, candidate proteases that could potentially provide hydrolase activity required for N remobilization from BSPs and provide the foundation for research to advance our knowledge of poplar N cycling. C1 [Islam, Nazrul; Li, Gen; Lin, Rongshuang; Coleman, Gary D.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. [Garrett, Wesley M.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Sriram, Ganesh] Univ Maryland, Dept Chem & Biomol Engn, College Pk, MD 20742 USA. [Cooper, Bret] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Coleman, GD (reprint author), Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. EM gcoleman@umd.edu FU National Science Foundation [IOS0922650] FX This work was supported in part by National Science Foundation Grant IOS0922650. NR 106 TC 0 Z9 0 U1 4 U2 26 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 EI 1535-3907 J9 J PROTEOME RES JI J. Proteome Res. PD FEB PY 2015 VL 14 IS 2 BP 1112 EP 1126 DI 10.1021/pr501090p PG 15 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CA9WV UT WOS:000349276400047 PM 25513840 ER PT J AU Karl, JP Thompson, LA Niro, PJ Margolis, LM McClung, JP Cao, JJ Whigham, LD Combs, GF Young, AJ Lieberman, HR Pasiakos, SM AF Karl, J. Philip Thompson, Lauren A. Niro, Philip J. Margolis, Lee M. McClung, James P. Cao, Jay J. Whigham, Leah D. Combs, Gerald F., Jr. Young, Andrew J. Lieberman, Harris R. Pasiakos, Stefan M. TI Transient decrements in mood during energy deficit are independent of dietary protein-to-carbohydrate ratio SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Macronutrient composition; Neurotransmitter precursor; Weight loss; Cognition; Sleep; Recommended dietary allowance ID SUBJECTIVE SLEEP QUALITY; WEIGHT-LOSS; COGNITIVE PERFORMANCE; OBESE WOMEN; BRAIN; TRIAL; RESTRICTION; MAINTENANCE; OVERWEIGHT; PRECURSOR AB Energy deficit and dietary macronutrient intake are thought to independently modulate cognition, mood and sleep. To what extent manipulating the dietary ratio of protein-to-carbohydrate affects mood, cognition and sleep during short-term energy deficit is undetermined. Using a randomized, block design, 39 non-obese young adults (21 +/- 1 years, BMI 25 +/- 1 kg/m(2)) consumed diets containing 0.8 g, 1.6 g or 2.4 g protein per kg body weight per day for 31 days. Carbohydrate intake was reduced to accommodate higher protein intakes while dietary fat was maintained at 30% of total energy intake. Cognitive performance, mood, self-reported sleep quality, and plasma amino add concentrations were periodically assessed during a 10-day energy balance period and a subsequent 21 -day, 40% energy deficit period. Anger, tension and total mood disturbance increased during the initial ten days of energy deficit (P < 0.05), but by the end of the energy deficit returned to levels not different from those measured during energy balance. No effects of dietary protein-to-carbohydrate ratio on cognitive performance, mood or self-reported sleep quality were observed during energy balance or energy deficit. Thus, high-protein, low-carbohydrate, moderate-fat diets do not appear to benefit or impair cognition, mood or sleep in non-obese adults during energy deficit. These findings suggest that energy deficit may initially be psychologically difficult for non-obese individuals attempting to lose weight, but that these changes are transient. Employing strategies that alleviate decrements in mood during this initial period of adaptation may help sustain weight loss efforts. Published by Elsevier Inc. C1 [Karl, J. Philip; Thompson, Lauren A.; Niro, Philip J.; Margolis, Lee M.; McClung, James P.; Young, Andrew J.; Lieberman, Harris R.; Pasiakos, Stefan M.] US Army, Mil Nutr Div, Environm Med Res Inst, Natick, MA 01760 USA. [Cao, Jay J.; Combs, Gerald F., Jr.] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58203 USA. [Whigham, Leah D.] Paso del Norte Inst Hlth Living, El Paso, TX 79968 USA. RP Pasiakos, SM (reprint author), US Army, Environm Med Res Inst, 15 Kansas St,Bldg 42, Natick, MA 01760 USA. EM james.p.karl@us.army.mil; lauren.a.thompson@us.army.mil; philip.niro@us.army.mil; lee.m.margolis.ctr@mail.mil; james.p.mcclung8.civ@mail.mil; jay.cao@ars.usda.gov; ldwhigham@utep.edu; gerald.combs@ars.usda.gov; andrew.j.young.civ@mail.mil; harris.lieberman@us.army.mil; stefan.pasiakos@us.army.mil RI Pasiakos, Stefan/E-6295-2014; Biguzzi, Felipe/E-4724-2015; OI Pasiakos, Stefan/0000-0002-5378-5820; Karl, J. Philip/0000-0002-5871-2241; , Lee/0000-0002-0652-1304; Whigham, Leah/0000-0002-5376-8967 FU U.S. Army Medical Research and Material Command; U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707] FX The U.S. Army Medical Research and Material Command and the U.S. Department of Agriculture, Agricultural Research Service, under agreement No. 58-1950-7-707. NR 44 TC 1 Z9 1 U1 4 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD FEB PY 2015 VL 139 BP 524 EP 531 DI 10.1016/j.physbeh.2014.11.068 PG 8 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA CB4BY UT WOS:000349574400072 PM 25479571 ER PT J AU Welch, KD Stonecipher, CA Gardner, DR Panter, KE Parsons, C Deboodt, T Johnson, B AF Welch, Kevin D. Stonecipher, Clint A. Gardner, Dale R. Panter, Kip E. Parsons, Cory Deboodt, Tim Johnson, Bryan TI The effect of western juniper on the estrous cycle in beef cattle SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Western juniper; Estrus; Estrous cycle; Abortion; Cattle; Isocupressic acid ID PINE NEEDLE ABORTION; PONDEROSA PINE; ISOCUPRESSIC ACID; LIVESTOCK; COMPOUND; TREES AB Numerous evergreen trees and shrubs contain labdane acids, including isocupressic acid, which can cause late-term abortions in cattle. Recent research has-shown that the bark from western juniper trees can also cause late-term abortions in cattle. Additionally, ranchers have observed that cattle in western juniper-infested rangelands tend to have decreased conception rates. The objective of this study was to determine if western juniper alters the estrous cycle of cattle. Fourteen heifers (10 treated and 4 control) were monitored for 74 days for signs of normal estrous behavior, with a 21 day feeding trial with western juniper bark from days 28-48, after which the cattle were bred naturally with a bull. The cattle were checked for pregnancy 30 days after all cattle had been bred. The data from this study indicate that exposure to western juniper bark does not affect normal estrus, estrous cycle or conception rates of cattle. Published by Elsevier Ltd. C1 [Welch, Kevin D.; Stonecipher, Clint A.; Gardner, Dale R.; Panter, Kip E.; Johnson, Bryan] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84321 USA. [Parsons, Cory] Oregon State Univ, Baker Cty Extens Serv, Baker City, OR USA. [Deboodt, Tim] Oregon State Univ, Crook Cty Extens Serv, Prineville, OR USA. RP Welch, KD (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84321 USA. EM Kevin.Welch@ars.usda.gov FU Oregon Beef Council; direct congressional appropriations via the United State Department of Agriculture FX This work was supported in part by the Oregon Beef Council and by direct congressional appropriations via the United State Department of Agriculture to the Poisonous Plant Research Laboratory, which is part of the Agricultural Research Service. NR 16 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD FEB PY 2015 VL 98 BP 16 EP 18 DI 10.1016/j.rvsc.2014.12.010 PG 3 WC Veterinary Sciences SC Veterinary Sciences GA CB4EZ UT WOS:000349582300004 PM 25554383 ER PT J AU Xu, SZ Lee, SH Lillehoj, HS Hong, YH Bravo, D AF Xu, Shouzhen Lee, Sung-Hyen Lillehoj, Hyun S. Hong, Yeong Ho Bravo, David TI Effects of dietary selenium on host response to necrotic enteritis in young broilers SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Selenium; Chicken; Necrotic enteritis; Cytokine; beta-defensin ID DIFFERENTIAL GENE-EXPRESSION; EIMERIA-MAXIMA INFECTION; PERFRINGENS ALPHA-TOXIN; CLOSTRIDIUM-PERFRINGENS; IMMUNE-RESPONSE; BETA-DEFENSINS; CHICKEN LINES; VITAMIN-E; VACCINATION; SUPPLEMENTATION AB The effects of dietary supplementation of young broiler chickens with an organic selenium (Se) formulation, B-Traxim Se, on experimental necrotic enteritis (NE) were studied. Chickens treated with three Se doses (0.25, 0.50, 1.00 mg/kg) from hatch were orally challenged with Eimeria maxima at 14 days of age followed by Clostridium perfringens to induce NE. Chickens fed with 0.50 mg/kg Se showed significantly increased body weights and antibody levels against NetB, and significantly reduced gut lesions compared with non-supplemented chickens. However, there were no significant differences in Eimeria oocyst shedding between the Se-treated and non-supplemented groups. Levels of IL-1 beta, IL-6, IL-8, iNOS, LITAF, TNFSF15, AvBD6, AvBD8, and AvBD13 transcripts were increased in the gut and spleen of at least one of the three Se-treated groups compared with the non-treated group. These results suggest that dietary supplementation of young broilers with Se might be beneficial to reduce the negative consequence of NE. (C) 2014 Published by Elsevier Ltd. C1 [Xu, Shouzhen] Qingdao Agr Univ, Coll Anim Sci & Vet Med, Qingdao 266109, Shandong, Peoples R China. [Xu, Shouzhen; Lee, Sung-Hyen; Lillehoj, Hyun S.] USDA ARS, Beltsville Agr Res Ctr, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Lee, Sung-Hyen] Natl Acad Agr Sci, Rural Dev Adm, Wanju Gun 565851, Jeollabuk Do, South Korea. [Hong, Yeong Ho] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. [Bravo, David] InVivo NSA, F-56250 St Nolff, France. RP Lillehoj, HS (reprint author), USDA ARS, Anim Biosci & Biotechnol Lab, Bldg 1043,BARC East, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU ARS-USDA [1265-32000-097-13T]; Pancosma S.A. [1265-32000-097-13T]; Next-Generation BioGreen 21 grant [PJ008084]; Rural Development Administration, Republic of Korea; Investigation of the functional activity; development of functional foods of Allium hookeri of Rural Development Administration, Republic of Korea [PJ010490]; Shandong Provincial Education Department, P.R. China FX This study was partially supported by a formal Trust agreement between ARS-USDA and Pancosma S.A. (1265-32000-097-13T), the Next-Generation BioGreen 21 grant (No. PJ008084), Rural Development Administration, Republic of Korea, and also partially supported by the grant 'Investigation of the functional activity and the development of functional foods of Allium hookeri (PJ010490)' of Rural Development Administration, Republic of Korea. Dr. Xu was supported by the International Cooperation Program for Excellent Lecturers of 2011 by Shandong Provincial Education Department, P.R. China. The authors thank Margie Nichols, Stacy O'Donnell, Seunglk Jang, Misun Jeong and SeungKyoo Lee, USDA-ARS, Beltsville, MD, for their valuable contributions to this study. NR 42 TC 0 Z9 0 U1 3 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD FEB PY 2015 VL 98 BP 66 EP 73 DI 10.1016/j.rvsc.2014.12.004 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA CB4EZ UT WOS:000349582300012 PM 25575447 ER PT J AU Wingeyer, AB Amado, TJC Perez-Bidegain, M Studdert, GA Varela, CHP Garcia, FO Karlen, DL AF Wingeyer, Ana B. Amado, Telmo J. C. Perez-Bidegain, Mario Studdert, Guillermo A. Perdomo Varela, Carlos H. Garcia, Fernando O. Karlen, Douglas L. TI Soil Quality Impacts of Current South American Agricultural Practices SO SUSTAINABILITY LA English DT Article; Proceedings Paper CT Sustainable Asia Conference 2014 CY APR 23-25, 2014 CL Nanchang, PEOPLES R CHINA ID NO-TILL; NITROGEN-FERTILIZATION; ORGANIC-CARBON; CROP ROTATIONS; USE INTENSITY; LAND-USE; SUSTAINABILITY; MANAGEMENT; MOLLISOLS; PAMPAS AB Increasing global demand for oil seeds and cereals during the past 50 years has caused an expansion in the cultivated areas and resulted in major soil management and crop production changes throughout Bolivia, Paraguay, Uruguay, Argentina and southern Brazil. Unprecedented adoption of no-tillage as well as improved soil fertility and plant genetics have increased yields, but the use of purchased inputs, monocropping i.e., continuous soybean (Glycine max (L.) Merr.), and marginal land cultivation have also increased. These changes have significantly altered the global food and feed supply role of these countries, but they have also resulted in various levels of soil degradation through wind and water erosion, soil compaction, soil organic matter (SOM) depletion, and nutrient losses. Sustainability is dependent upon local interactions between soil, climate, landscape characteristics, and production systems. This review examines the region's current soil and crop conditions and summarizes several research studies designed to reduce or prevent soil degradation. Although the region has both environmental and soil resources that can sustain current agricultural production levels, increasing population, greater urbanization, and more available income will continue to increase the pressure on South American croplands. A better understanding of regional soil differences and quantifying potential consequences of current production practices on various soil resources is needed to ensure that scientific, educational, and regulatory programs result in land management recommendations that support intensification of agriculture without additional soil degradation or other unintended environmental consequences. C1 [Wingeyer, Ana B.] INTA, Estn Expt Agr Parana, RA-3101 Oro Verde, Entre Rios, Argentina. [Amado, Telmo J. C.] Univ Fed Santa Maria, Ctr Ciencias Rurais, BR-97105900 Santa Maria, RS, Brazil. [Perez-Bidegain, Mario; Perdomo Varela, Carlos H.] Univ Republica, Fac Agron, Montevideo 12900, Uruguay. [Studdert, Guillermo A.] Univ Nacl Mar Plata, Fac Ciencias Agr, Unidad Integrada Balcarce, RA-7620 Buenos Aires, DF, Argentina. [Garcia, Fernando O.] IPNI Latinoamer Cono Sur, Buenos Aires, DF, Argentina. [Karlen, Douglas L.] USDA ARS, NLAE, Ames, IA 50011 USA. RP Wingeyer, AB (reprint author), INTA, Estn Expt Agr Parana, Ruta 11,Km 12,5, RA-3101 Oro Verde, Entre Rios, Argentina. EM wingeyer.ana@inta.gob.ar; telmo.amado@pq.cnpq.br; mperezb@fagro.edu.uy; studdert.guillermo@inta.gob.ar; chperdom@fagro.edu.uy; FGarcia@ipni.net; Doug.Karlen@ars.usda.gov RI Amado, Telmo /B-9909-2008; Amado, Telmo /C-4445-2014; OI Amado, Telmo /0000-0001-8417-9009; Amado, Telmo /0000-0001-8417-9009; Wingeyer, Ana/0000-0001-5579-9532 NR 85 TC 9 Z9 9 U1 10 U2 75 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD FEB PY 2015 VL 7 IS 2 BP 2213 EP 2242 DI 10.3390/su7022213 PG 30 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA CC3AY UT WOS:000350217700058 ER PT J AU Li, HK Wan, YZ Simon, CJ AF Li, Huike Wan, Yi-zhen Simon, Charles J. TI Patterns of SNP distribution provide a molecular basis for high genetic diversity and genetic differentiation in Vitis species SO TREE GENETICS & GENOMES LA English DT Article DE Grapes; Nucleotide diversity; Analysis of molecular variance; Genetic relationships; Genetic differentiation; Nei's algorithm ID SINGLE-NUCLEOTIDE POLYMORPHISMS; LINKAGE DISEQUILIBRIUM; VINIFERA L.; PHYLOGENETIC ANALYSIS; POPULATION-STRUCTURE; MULTILOCUS PATTERNS; DEMOGRAPHIC HISTORY; CLIMATE-CHANGE; WILD GRAPES; CONSERVATION AB Grape (Vitis L.) is one of the most economically valuable fruit crops in the world. The molecular basis for high genetic diversity and genetic differentiation thus conferring broad adaptability in the Vitis genus remains elusive. Genetic patterns in Vitis were comprehensively characterized by re-sequencing of 6862 nuclear SNPs in this study. Nucleotide diversity (pi=0.0073), genetic differentiation (pairwise differences, 0.24) and SNP variation in Vitis species were relatively high compared to most woody plant species. Molecular variance among accessions within species (F-IS=0.369) and among 30 species (F-ST=0.511) suggested strong population structure in Vitis (P<0.0001). Vitis species are characterized by low frequency (4.7 %) of most minor SNPs and high proportion (> 95 %) of minor SNPs to engender high differentiation among species thus high genetic diversity in the whole genus. This confers potentially broad adaptability thus a broad base for natural selection in this genus. Species from three geographical regions were discerned into independent quadrants in the principle coordinate analysis (PCoA) plot and their clustered patterns suggested that their molecular identities represented geographical origins of Vitis species. Species extinction will be an irreversible loss for breeding and genetic conservation. Species with low diversity, low heterozygosity, and high agronomical value should be given priority for conservation. Survey of species-specific SNPs will facilitate germplasm conservation and grape breeding programs. This study greatly adds to our knowledge of genetic patterns and molecular bases for ecological habits of Vitis species and provides valuable information for germplasm conservation and utilization, and grape breeding. C1 [Wan, Yi-zhen] Northwest A&F Univ, Coll Hort, Xianyang 712100, Shaanxi, Peoples R China. [Simon, Charles J.] Cornell Univ, USDA, ARS, Plant Genet Resources Unit,New York State Agr Exp, Geneva, NY 14456 USA. [Li, Huike] Northwest A&F Univ, Coll Environm Resources, Xianyang 712100, Shaanxi, Peoples R China. RP Wan, YZ (reprint author), Northwest A&F Univ, Coll Hort, Xianyang 712100, Shaanxi, Peoples R China. EM 1036520399@qq.com FU National Yangling Bio-Tech Breeding Centre Project [2001-01]; China Scholarship Council [22861057]; Young Scientist Foundation of NWAFU [QN2009-013]; Fund for Returnees Scientists from Foreign Study in Shaanxi Province [A289021201]; USDA-ARS Plant Genetic Resources Unit CRIS Project [1910-21000-020-00D] FX We are grateful to Dr. Joanne A. Labate for making valuable comments and suggestions about this study, to Angela M. Baldo for designing primers for resequencing, to Dr. Peter Cousins (E. &. J. Gallo Winery) for providing expert advice on grape identification and classification, and to Dr. Gan-Yuan Zhong for valuable discussion. USDA provided equal contribution to this study. This work was supported by the National Yangling Bio-Tech Breeding Centre Project (2001-01), China Scholarship Council Project (22861057), the Young Scientist Foundation of NWAFU (QN2009-013), the 2012-Fund for Returnees Scientists from Foreign Study in Shaanxi Province (A289021201) and the USDA-ARS Plant Genetic Resources Unit CRIS Project 1910-21000-020-00D. USDA is an equal opportunity provider and employer. NR 78 TC 0 Z9 0 U1 2 U2 35 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD FEB PY 2015 VL 11 IS 1 AR 13 DI 10.1007/s11295-015-0832-7 PG 17 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CB3UD UT WOS:000349553500012 ER PT J AU Zerega, N Wiesner-Hanks, T Ragone, D Irish, B Scheffler, B Simpson, S Zee, F AF Zerega, Nyree Wiesner-Hanks, Tyr Ragone, Diane Irish, Brian Scheffler, Brian Simpson, Sheron Zee, Francis TI Diversity in the breadfruit complex (Artocarpus, Moraceae): genetic characterization of critical germplasm SO TREE GENETICS & GENOMES LA English DT Article DE Breadfruit; Artocarpus; Germplasm conservation management; Microsatellites; Plant genetic resources; Underutilized crops ID MULTILOCUS GENOTYPE DATA; MICROSATELLITE MARKERS; COMPUTER-PROGRAM; CULTIVARS; IDENTIFICATION; RAPD; POLYPLOIDS; MANAGEMENT; PACIFIC; ALTILIS AB Breadfruit (Artocarpus altilis, Moraceae) is a traditional staple crop in Oceania and has been introduced throughout the tropics. This study examines important germplasm collections of breadfruit and its closest wild relatives and aims to (1) characterize genetic diversity, including identification of unknown and duplicate accessions, (2) evaluate genetic structure and hybridization within the breadfruit complex, and (3) compare utility of microsatellite markers to previously reported amplified fragment length polymorphism (AFLP) and isozyme markers in differentiating among cultivars. Data for 19 microsatellite loci were collected for 349 individuals (representing 255 accessions) including breadfruit (A. altilis), two wild relatives (Artocarpus camansi and Artocarpus mariannensis), and putative hybrids (A. altilis x A. mariannensis). Accessions were of mixed ploidy and regional origin, but predominantly from Oceania. Microsatellite loci collectively had a polymorphic information content (PIC) of 0.627 and distinguished 197 unique genotypes sorted into 129 different lineages, but a single genotype accounts for 49 % of all triploid breadfruit examined. Triploid hybrids and diploid A. altilis exhibited the highest levels of diversity as measured by allele number and gene diversity. Most accessions (75 %) of unknown origin matched either a known genotype or lineage group in the collection. Putative hybrids all had genetic contributions from A. mariannensis but ranged in the level of genetic contribution from A. altilis. Microsatellite markers were found to be more informative than isozyme markers and slightly less informative, with regard to accession discrimination, than AFLP markers. This set of microsatellite markers and the dataset presented here will be valuable for breadfruit germplasm management and conservation. C1 [Zerega, Nyree; Wiesner-Hanks, Tyr] Northwestern Univ, Evanston, IL 60208 USA. [Zerega, Nyree] Chicago Bot Garden, Dept Plant Sci, Glencoe, IL 60022 USA. [Ragone, Diane] Natl Trop Bot Garden, Breadfruit Inst, Kalaheo, HI 96741 USA. [Irish, Brian] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Scheffler, Brian; Simpson, Sheron] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Zee, Francis] USDA ARS, PBARC, Hilo, HI 96720 USA. RP Zerega, N (reprint author), Northwestern Univ, Evanston, IL 60208 USA. EM nzerega@chicagobotanic.org OI Scheffler, Brian/0000-0003-1968-8952 FU National Science Foundation [DEB-0919119]; USDA/ARS; Chicago Botanic Garden FX The authors thank the Breadfruit Institute and the USDA/ARS National Plant Germplasm System for use of plant material, Ian Cole for collecting the samples at Kahanu Garden at NTBG, the Trustees and Fellows of NTBG for their support of the Breadfruit Institute, and two reviewers who provided valuable feedback that improved the manuscript. The research was made possible in part by National Science Foundation Grant DEB-0919119 and support from USDA/ARS and the Chicago Botanic Garden. NR 65 TC 4 Z9 4 U1 2 U2 22 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD FEB PY 2015 VL 11 IS 1 AR 4 DI 10.1007/s11295-014-0824-z PG 26 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA CB3UD UT WOS:000349553500003 ER PT J AU Burner, DM Brauer, DK Pote, DH Snider, JL AF Burner, D. M. Brauer, D. K. Pote, D. H. Snider, J. L. TI Effect of understory management on phenological responses of eastern black walnut on an alluvial Arkansas soil SO AGROFORESTRY SYSTEMS LA English DT Article DE Phenology; Fertilzer; Leaf drop; Bud break; Leaf area ID LOBLOLLY-PINE ALLEY; GAS-EXCHANGE; TALL FESCUE; WHITE OAK; LEAF; NITROGEN; GROWTH; TREES; CULTIVARS; SEEDLINGS AB Black walnut (Juglans nigra L.) is commonly grown in agroforestry practices for nuts and/or timber with little knowledge of how understory herbage management might affect tree phenology. We compared black walnut plant type (variety and wild-type) for phenological response in date of budburst, leaf area index (LAI), quantum yield of photosystem II (Fv/Fm), radial stem growth, and total chlorophyll concentration in response to understory herbage N fertilization and herbage suppression in an agroforestry practice on an alluvial soil. Herbage treatments generally had little impact on phenological responses, although N fertilization increased herbage yield that delayed budburst. Radial stem growth commenced at about budburst (mid-April) and virtually ceased in late October, about 30 days before leaf drop. Early-season radial stem growth was greater for the wild-type than the variety, while the variety had more rapid late-season growth. The wild-type had consistently greater LAI than the variety at any given day-of-year (DOY), and usually had greater LAI than the variety regardless of herbage treatment. Leaves of both plant types maintained a high Fv/Fm during most of the growing season, but total chlorophyll concentration decreased across DOY regardless of plant type. N fertilizer should be applied with understory herbage suppression to avoid delay of budburst. Further study is needed of phenological responses during longer growth periods, or as affected by latitude, soil water, and soil fertility. C1 [Burner, D. M.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. [Brauer, D. K.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Pote, D. H.] USDA ARS, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Snider, J. L.] Univ Georgia, Tifton, GA 31794 USA. RP Brauer, DK (reprint author), USDA ARS, Conservat & Prod Res Lab, 2300 Expt Stn Rd,PO Drawer 10, Bushland, TX 79012 USA. EM david.burner@ars.usda.gov; david.brauer@ars.usda.gov; dan.pote@ars.usda.gov; jlsnider@uga.edu FU USDA-ARS, Dale Bumpers Small Farms Research Center, Booneville, Arkansas [6227-21660-003-00D] FX USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. Authors were formerly located at the Dale Bumpers Small Farms Research Center, 6883 South State Hwy. 23, Booneville, Arkansas 72927. Expert assistance was provided by Dr. D.E. Brauer and Ms. T. Horton (ARS, Booneville), and Mr. Randy King (NRCS, Booneville). Dr. D.H. Pote (ARS, Booneville) conducted the soil classification. Technical expertise was provided by K. Chapman, L. Huddleston, J. Whiley, and B. Woolley. Funding for the research was provided by USDA-ARS, Dale Bumpers Small Farms Research Center, Booneville, Arkansas through Project 6227-21660-003-00D. NR 61 TC 0 Z9 0 U1 3 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD FEB PY 2015 VL 89 IS 1 BP 135 EP 147 DI 10.1007/s10457-014-9748-8 PG 13 WC Agronomy; Forestry SC Agriculture; Forestry GA CA5VR UT WOS:000348976400012 ER PT J AU Lan, PX Li, F Wang, MQ Li, RH AF Lan, Pingxiu Li, Fan Wang, Mingqiang Li, Ruhui TI Complete genome sequence of a divergent strain of Japanese yam mosaic virus from China SO ARCHIVES OF VIROLOGY LA English DT Article ID COMPLETE NUCLEOTIDE-SEQUENCE; POTYVIRUSES; POTYVIRIDAE; RNA; PCR AB A novel strain of Japanese yam mosaic virus (JYMV-CN) was identified in a yam plant with foliar mottle symptoms in China. The complete genomic sequence of JYMV-CN was determined. Its genomic sequence of 9701 nucleotides encodes a polyprotein of 3247 amino acids. Its organization is virtually identical to that of two JYMV isolates from Japan. With the latter, it shares nucleotide sequence identities of only 74.7-74.8 %, indicating it might be a member of a new species. However, sequence analysis of the polyprotein and individual proteins suggested that the Chinese isolate is a divergent JYMV strain in the process of speciation. C1 [Lan, Pingxiu; Wang, Mingqiang; Li, Ruhui] ARS, USDA, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Lan, Pingxiu; Li, Fan] Yunnan Agr Univ, Kunming 650201, Yunnan, Peoples R China. RP Li, RH (reprint author), ARS, USDA, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. EM Ruhui.Li@ars.usda.gov NR 18 TC 1 Z9 1 U1 0 U2 2 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD FEB PY 2015 VL 160 IS 2 BP 573 EP 576 DI 10.1007/s00705-014-2231-z PG 4 WC Virology SC Virology GA CB2CE UT WOS:000349433500031 PM 25244960 ER PT J AU Vilchis, LI Johnson, CK Evenson, JR Pearson, SF Barry, KL Davidson, P Raphael, MG Gaydos, JK AF Vilchis, L. Ignacio Johnson, Christine K. Evenson, Joseph R. Pearson, Scott F. Barry, Karen L. Davidson, Peter Raphael, Martin G. Gaydos, Joseph K. TI Assessing ecological correlates of marine bird declines to inform marine conservation SO CONSERVATION BIOLOGY LA English DT Article DE community ecology; epidemiology; forage fish; marine birds; pursuit divers; risk analysis; analisis de riesgo; aves marinas; ecologia de comunidades; epidemiologia; peces forrajeros; pescadores de persecucion ID BRITISH-COLUMBIA; PUGET-SOUND; PACIFIC; SEABIRD; ABUNDANCE; IMPACTS; TRENDS; EXTINCTION; WASHINGTON; ECOSYSTEMS AB Identifying drivers of ecosystem change in large marine ecosystems is central for their effective management and conservation. This is a sizable challenge, particularly in ecosystems transcending international borders, where monitoring and conservation of long-range migratory species and their habitats are logistically and financially problematic. Here, using tools borrowed from epidemiology, we elucidated common drivers underlying species declines within a marine ecosystem, much in the way epidemiological analyses evaluate risk factors for negative health outcomes to better inform decisions. Thus, we identified ecological traits and dietary specializations associated with species declines in a community of marine predators that could be reflective of ecosystem change. To do so, we integrated count data from winter surveys collected in long-term marine bird monitoring programs conducted throughout the Salish Seaa transboundary large marine ecosystem in North America's Pacific Northwest. We found that decadal declines in winter counts were most prevalent among pursuit divers such as alcids (Alcidae) and grebes (Podicipedidae) that have specialized diets based on forage fish, and that wide-ranging species without local breeding colonies were more prone to these declines. Although a combination of factors is most likely driving declines of diving forage fish specialists, we propose that changes in the availability of low-trophic prey may be forcing wintering range shifts of diving birds in the Salish Sea. Such a synthesis of long-term trends in a marine predator community not only provides unique insights into the types of species that are at risk of extirpation and why, but may also inform proactive conservation measures to counteract threatsinformation that is paramount for species-specific and ecosystem-wide conservation. Evaluacion de las Correlaciones Ecologicas de las Declinaciones de Aves Marinas para Informar a la Conservacion Marina Resumen La identificacion de los conductores del cambio ambiental en los grandes ecosistemas marinos es esencial para su conservacion y manejo efectivo. Esto es un reto bastante grande, particularmente en los ecosistemas que trascienden fronteras internacionales, cuando el monitoreo y la conservacion de especies migratorias de amplio rango y sus habitats son logistica y financieramente problematicos. En este caso, usando herramientas tomadas de la epidemiologia, elucidamos conductores comunes subyacentes en la declinacion de especies dentro de un ecosistema marino, muy similar a como los analisis epidemiologicos evaluan los factores de riesgo para los resultados de salud negativos e informar mejor sus decisiones. Con esto, identificamos los rasgos ecologicos y las especializaciones de dieta asociados con la declinacion de especies en una comunidad de depredadores marinos que podria ser un reflejo de cambios ambientales. Para lograr esto, integramos datos de conteo de programas de censos de invierno recolectados a lo largo de monitoreos a largo plazo de aves marinas llevados a cabo en el mar Salish - un gran ecosistema marino que trasciende fronteras en el noroeste del Oceano Pacifico. Encontramos que las declinaciones por decada en los conteos de invierno fueron mas prevalentes entre los pescadores de persecucion, como los alcidos (Alcidae) y los zambullidores (Podicipedidae), que tienen dietas especializadas basadas en peces forrajeros y que las especies con distribucion amplia y sin colonias reproductivas locales estaban mas predispuestas a estas declinaciones. Mientras que una combinacion de factores posiblemente este causando las declinaciones de especialistas de peces forrajeros, proponemos que los cambios en la disponibilidad de presas de niveles troficos bajos pueden estar forzando cambios en la extension invernal de aves pescadoras en el mar Salish. Dicha sintesis de tendencias a largo plazo en una comunidad de depredadores marinos no solo proporciona percepciones unicas de este tipo de especies que estan en riesgo de ser extirpadas y el por que de esto, sino tambien puede informar a las medidas de conservacion proactivas para contrarrestar amenazas - informacion que es primordial para la conservacion especifica de especies y del ecosistema en su totalidad. C1 [Vilchis, L. Ignacio; Johnson, Christine K.; Gaydos, Joseph K.] Univ Calif Davis, Sch Vet Med, Wildlife Hlth Ctr, Davis, CA 95616 USA. [Evenson, Joseph R.; Pearson, Scott F.] Washington Dept Fish & Wildlife, Wildlife Res Div, Olympia, WA 98501 USA. [Barry, Karen L.; Davidson, Peter] Bird Studies Canada, Pacific Wildlife Res Ctr, Delta, BC V4K 3N2, Canada. [Raphael, Martin G.] US Forest Serv, USDA, Pacific NW Res Stn, Olympia, WA 98512 USA. RP Vilchis, LI (reprint author), Univ Calif Davis, Sch Vet Med, Wildlife Hlth Ctr, Davis, CA 95616 USA. EM lvilchis@ucsd.edu OI Gaydos, Joseph/0000-0001-6599-8797 FU SeaDoc Society-a program of the UC Davis Wildlife Health Center; Canadian Wildlife Service through Bird Studies Canada; Washington State FX This research was funded by the SeaDoc Society-a program of the UC Davis Wildlife Health Center. The Canadian Wildlife Service through Bird Studies Canada provided long-term funding for the BCCWS, and aerial surveys of the WDFW were funded by Washington State. Success of long-term, large-scale studies like ours relies on collaborations between multiple organizations spanning an international border, and the continuous participation of pilots, observers, and volunteers spearheading various monitoring programs-without them this research would be impossible. We are also grateful to Audubon's G. LeBaron and K. Dale for graciously giving us access to long-term CBC data for British Columbia and Washington State. Conversations with J. Parrish and A. Punt and reviews by J. McGowan and J. Piatt significantly improved our manuscript. NR 44 TC 5 Z9 5 U1 8 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD FEB PY 2015 VL 29 IS 1 BP 154 EP 163 DI 10.1111/cobi.12378 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA2AX UT WOS:000348712400018 PM 25195954 ER PT J AU Wittmann, ME Cooke, RM Rothlisberger, JD Rutherford, ES Zhang, H Mason, DM Lodge, DM AF Wittmann, Marion E. Cooke, Roger M. Rothlisberger, John D. Rutherford, Edward S. Zhang, Hongyan Mason, Doran M. Lodge, David M. TI Use of structured expert judgment to forecast invasions by bighead and silver carp in Lake Erie SO CONSERVATION BIOLOGY LA English DT Article DE Asian carp; ecological forecasting; invasive species; Laurentian Great Lakes; risk assessment; carpa asiatica; especies invasoras; evaluacion de riesgo; Grandes Lagos; prediccion ecologica ID CLASSICAL-MODEL; ASIAN CARPS; RISK; INVADERS AB Identifying which nonindigenous species will become invasive and forecasting the damage they will cause is difficult and presents a significant problem for natural resource management. Often, the data or resources necessary for ecological risk assessment are incomplete or absent, leaving environmental decision makers ill equipped to effectively manage valuable natural resources. Structured expert judgment (SEJ) is a mathematical and performance-based method of eliciting, weighting, and aggregating expert judgments. In contrast to other methods of eliciting and aggregating expert judgments (where, for example, equal weights may be assigned to experts), SEJ weights each expert on the basis of his or her statistical accuracy and informativeness through performance measurement on a set of calibration variables. We used SEJ to forecast impacts of nonindigenous Asian carp (Hypophthalmichthys spp.) in Lake Erie, where it is believed not to be established. Experts quantified Asian carp biomass, production, and consumption and their impact on 4 fish species if Asian carp were to become established. According to experts, in Lake Erie Asian carp have the potential to achieve biomass levels that are similar to the sum of biomasses for several fishes that are harvested commercially or recreationally. However, the impact of Asian carp on the biomass of these fishes was estimated by experts to be small, relative to long term average biomasses, with little uncertainty. Impacts of Asian carp in tributaries and on recreational activities, water quality, or other species were not addressed. SEJ can be used to quantify key uncertainties of invasion biology and also provide a decision-support tool when the necessary information for natural resource management and policy is not available. El Uso de Juicio Experto Estructurado para Predecir Invasiones de Carpas Asiaticas en el Lago Erie Resumen Identificar cuales especies no-nativas se volveran invasoras y predecir el dano que causaran es complicado y presenta un problema significativo para el manejo de recursos naturales. Con frecuencia los datos o recursos necesarios para la evaluacion de riesgo ecologico estan incompletos o son inexistentes, lo que deja mal equipados a quienes toman las decisiones ambientales para manejar efectivamente recursos naturales valiosos. El juicio experto estructurado (JEE) es un metodo con bases matematicas y de desempeno para obtener, sopesar y agregar juicios expertos. En contraste con otros metodos de obtencion y agregacion de juicios expertos (donde, por ejemplo, se le pueden asignar pesos iguales a los expertos), JEE sopesa a cada experto con base en su asertividad estadistica y capacidad de informar por medio de la medida de desempeno en un conjunto de variables de calibracion. Usamos JEE para predecir los impactos de las carpas asiaticas no-nativas Hypophthalmichthys spp. en el Lago Erie, donde se cree que no se ha establecido. Los expertos cuantificaron la biomasa, produccion y consumo de la carpa asiatica y su impacto sobre cuatro especies de peces si la carpa asiatica se llegara a establecer en el lago. De acuerdo a los expertos, en el Lago Erie, la carpa asiatica tiene el potencial de adquirir niveles de biomasa similares a la suma de biomasa de varios peces que se han cultivado comercialmente o recreativamente. Sin embargo, se estimo por los expertos que el impacto de la carpa asiatica sobre la biomasa de estos peces seria pequeno, con poca incertidumbre. Los impactos de la carpa asiatica sobre los tributarios y las actividades recreativas, la calidad del agua o sobre otras especies no se evaluaron. El JEE puede usarse para cuantificar incertidumbres clave de la biologia de la invasion y tambien proporcionar una herramienta de apoyo para las decisiones cuando la informacion necesaria para el manejo de los recursos naturales y la politica no esta disponible. C1 [Wittmann, Marion E.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46656 USA. [Cooke, Roger M.] Resources Future Inc, Washington, DC 20036 USA. [Cooke, Roger M.] Delft Univ Technol, NL-2628 CN Delft, Netherlands. [Cooke, Roger M.] Univ Strathclyde, Glasgow G1 1XQ, Lanark, Scotland. [Rothlisberger, John D.] US Forest Serv, Milwaukee, WI 53202 USA. [Rutherford, Edward S.; Mason, Doran M.] NOAA, Great Lakes Environm Res Lab, Ann Arbor, MI 48108 USA. [Zhang, Hongyan] Univ Michigan, Cooperat Inst Limnol & Ecosyst Res SNRE, Ann Arbor, MI 48108 USA. [Lodge, David M.] Dept Biol Sci, Notre Dame, IN 46656 USA. [Lodge, David M.] Environm Change Initiat, Notre Dame, IN 46656 USA. RP Wittmann, ME (reprint author), Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46656 USA. EM mwittmann@gmail.com OI Mason, Doran/0000-0002-6017-4243; Rutherford, Edward/0000-0002-7282-6667 FU Environmental Protection Agency Great Lakes Restoration Initiative, NOAA Center for Sponsored Coastal Ocean Research awards [NA09NOS4780192, NA10NOS4780218] FX We thank the experts for their dedicated and focused response to the elicitation. This research was funded by Environmental Protection Agency Great Lakes Restoration Initiative, NOAA Center for Sponsored Coastal Ocean Research awards NA09NOS4780192 and NA10NOS4780218. We thank A. Deines for his support in the preparation of the elicitation. This is NOAA-GLERL contribution 1716 and a publication of the Notre Dame Environmental Change Initiative. NR 42 TC 8 Z9 9 U1 10 U2 75 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD FEB PY 2015 VL 29 IS 1 BP 187 EP 197 DI 10.1111/cobi.12369 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA2AX UT WOS:000348712400021 PM 25132396 ER PT J AU Lebuhn, G Droege, S Connor, EF Gemmill-Herren, B Potts, SG Minckley, RL Jean, RP Kula, E Roubik, DW Wright, KW Frankie, G Parker, F AF Lebuhn, Gretchen Droege, Sam Connor, Edward F. Gemmill-Herren, Barbara Potts, Simon G. Minckley, Robert L. Jean, Robert P. Kula, Emanuel Roubik, David W. Wright, Karen W. Frankie, Gordon Parker, Frank TI Evidence-based conservation: reply to Tepedino et al. SO CONSERVATION BIOLOGY LA English DT Editorial Material C1 [Lebuhn, Gretchen; Connor, Edward F.] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA. [Droege, Sam] USGS Patuxent Wildlife Res Ctr, Laurel, MD 20708 USA. [Gemmill-Herren, Barbara] Food & Agr Org United Nations, I-00100 Rome, Italy. [Potts, Simon G.] Univ Reading, Sch Agr Policy & Dev, Ctr Agri Environm Res CAER, Reading RG6 6AR, Berks, England. [Minckley, Robert L.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA. [Jean, Robert P.] St Mary Of The Woods Coll, Dept Sci & Math, St Mary Of The Woods, IN 47876 USA. [Kula, Emanuel] Mendel Univ Brno, Fac Forestry & Wood Technol, Brno 134127, Czech Republic. [Roubik, David W.] Smithsonian Trop Res Inst, Balboa Ancon, Panama. [Wright, Karen W.] Univ New Mexico, Dept Biol, Sevilleta LTER, Albuquerque, NM 87131 USA. [Frankie, Gordon] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Parker, Frank] Utah State Univ, ARS, USDA, Bee Biol & Systemat Lab, Logan, UT 84322 USA. RP Lebuhn, G (reprint author), San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA. EM lebuhn@sfsu.edu FU Biotechnology and Biological Sciences Research Council NR 7 TC 2 Z9 2 U1 3 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0888-8892 EI 1523-1739 J9 CONSERV BIOL JI Conserv. Biol. PD FEB PY 2015 VL 29 IS 1 BP 283 EP 285 DI 10.1111/cobi.12438 PG 3 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA2AX UT WOS:000348712400031 PM 25545768 ER PT J AU Stewart, KM Walsh, DR Kie, JG Dick, BL Bowyer, RT AF Stewart, Kelley M. Walsh, Danielle R. Kie, John G. Dick, Brian L. Bowyer, R. Terry TI Sexual segregation in North American elk: the role of density dependence SO ECOLOGY AND EVOLUTION LA English DT Article DE Cervus elaphus; density dependence; experimental manipulation; intrasexual competition; North American elk; resource selection; sexual segregation ID DEER CERVUS-ELAPHUS; RESOURCE SELECTION FUNCTIONS; SOUTHERN MULE DEER; WHITE-TAILED DEER; HABITAT SELECTION; ALASKAN MOOSE; BIGHORN SHEEP; POPULATION-DENSITY; MOUNTAIN LIONS; TRADE-OFFS AB We investigated how density-dependent processes and subsequent variation in nutritional condition of individuals influenced both timing and duration of sexual segregation and selection of resources. During 1999-2001, we experimentally created two population densities of North American elk (Cervus elaphus), a high-density population at 20elk/km(2), and a low-density population at 4elk/km(2) to test hypotheses relative to timing and duration of sexual segregation and variation in selection of resources. We used multi-response permutation procedures to investigate patterns of sexual segregation, and resource selection functions to document differences in selection of resources by individuals in high- and low-density populations during sexual segregation and aggregation. The duration of sexual segregation was 2months longer in the high-density population and likely was influenced by individuals in poorer nutritional condition, which corresponded with later conception and parturition, than at low density. Males and females in the high-density population overlapped in selection of resources to a greater extent than in the low-density population, probably resulting from density-dependent effects of increased intraspecific competition and lower availability of resources. C1 [Stewart, Kelley M.; Walsh, Danielle R.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Kie, John G.; Bowyer, R. Terry] Idaho State Univ, Dept Biol Sci, Pocatello, ID 83209 USA. [Dick, Brian L.] US Forest Serv, Pacific NW Res Stn, La Grande, OR 97850 USA. RP Stewart, KM (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. EM kstewart@cabnr.unr.edu FU Pacific Northwest Research Station of the US Forest Service; Institute of Arctic Biology; University of Alaska Fairbanks; Rocky Mountain Elk Foundation; Rob and Bessie Welder Wildlife Foundation; Ecology, Evolution, and Conservation Biology Program; Department of Natural Resources and Environmental Science at the University of Nevada Reno; Department of Biological Sciences at Idaho State University FX Funding and support for the project were provided by the Pacific Northwest Research Station of the US Forest Service, the Institute of Arctic Biology and the University of Alaska Fairbanks, Rocky Mountain Elk Foundation, Rob and Bessie Welder Wildlife Foundation, Ecology, Evolution, and Conservation Biology Program and Department of Natural Resources and Environmental Science at the University of Nevada Reno, and Department of Biological Sciences at Idaho State University. NR 76 TC 5 Z9 5 U1 5 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD FEB PY 2015 VL 5 IS 3 BP 709 EP 721 DI 10.1002/ece3.1397 PG 13 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA CB1KK UT WOS:000349386000015 PM 25691992 ER PT J AU Johnson, RC Horning, ME Espeland, EK Vance-Borland, K AF Johnson, Richard C. Horning, Matthew E. Espeland, Erin K. Vance-Borland, Ken TI Relating adaptive genetic traits to climate for Sandberg bluegrass from the intermountain western United States SO EVOLUTIONARY APPLICATIONS LA English DT Article DE climate; genecology; plant adaptation; Poa secunda; restoration; seed zones ID POA-SECUNDA COMPLEX; LOCAL ADAPTATION; SEED TRANSFER; POPULATION DIFFERENTIATION; CRESTED WHEATGRASS; NORTH-AMERICA; DOUGLAS-FIR; GREAT-BASIN; GENECOLOGY; ZONES AB Genetic variation for potentially adaptive traits of the key restoration species Sandberg bluegrass (Poa secunda J. Presl) was assessed over the intermountain western United States in relation to source population climate. Common gardens were established at two intermountain west sites with progeny from two maternal parents from each of 130 wild populations. Data were collected over 2years at each site on fifteen plant traits associated with production, phenology, and morphology. Analyses of variance revealed strong population differences for all plant traits (P<0.0001), indicating genetic variation. Both the canonical correlation and linear correlation established associations between source populations and climate variability. Populations from warmer, more arid climates had generally lower dry weight, earlier phenology, and smaller, narrower leaves than those from cooler, moister climates. The first three canonical variates were regressed with climate variables resulting in significant models (P<0.0001) used to map 12 seed zones. Of the 700981km(2) mapped, four seed zones represented 92% of the area in typically semi-arid and arid regions. The association of genetic variation with source climates in the intermountain west suggested climate driven natural selection and evolution. We recommend seed transfer zones and population movement guidelines to enhance adaptation and diversity for large-scale restoration projects. C1 [Johnson, Richard C.] ARS, USDA, Pullman, WA USA. [Horning, Matthew E.] ARS, USDA, Bend, OR USA. [Espeland, Erin K.] ARS, USDA, Pest Management Res Unit, Sidney, MT USA. [Vance-Borland, Ken] Conservat Planning Inst, Corvallis, OR USA. RP Johnson, RC (reprint author), Washington State Univ, ARS, USDA, Rm 59 Johnson Hall, Pullman, WA 99164 USA. EM rcjohnson@wsu.edu FU USDI Bureau of Land Management Great Basin Restoration Initiative; USDA Forest Service Rocky Mountain Research Station's Great Basin Native Plant Selection and Increase Project FX Thanks to Melissa Scholten and Tamra Putensen for data collection at the Central Ferry garden and to Elise Brown, Jeff Riddle, Chris Poklemba, and the Central Oregon Youth Conservation Corps (YCC) for assistance with data collection in the Powell Butte garden. Funding was provided in part by the USDI Bureau of Land Management Great Basin Restoration Initiative, and the USDA Forest Service Rocky Mountain Research Station's Great Basin Native Plant Selection and Increase Project. NR 55 TC 1 Z9 1 U1 3 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1752-4571 J9 EVOL APPL JI Evol. Appl. PD FEB PY 2015 VL 8 IS 2 BP 172 EP 184 DI 10.1111/eva.12240 PG 13 WC Evolutionary Biology SC Evolutionary Biology GA CA7SL UT WOS:000349117300005 PM 25685192 ER PT J AU Liu, CY Dupuis, J Larson, MG Cupples, LA Ordovas, JM Vasan, RS Meigs, JB Jacques, PF Levy, D AF Liu, Chunyu Dupuis, Josee Larson, Martin G. Cupples, L. Adrienne Ordovas, Jose M. Vasan, Ramachandran S. Meigs, James B. Jacques, Paul F. Levy, Daniel TI Revisiting heritability accounting for shared environmental effects and maternal inheritance SO HUMAN GENETICS LA English DT Article ID HAN CHINESE FAMILY; MITOCHONDRIAL-DNA; VARIANCE-COMPONENTS; ESSENTIAL-HYPERTENSION; BLOOD-PRESSURE; HUMAN-DISEASE; MUTATION; ASSOCIATION; TRAITS; PEDIGREE AB Heritability measures the proportion of phenotypic variation attributable to genetic factors. In addition to a shared nuclear genetic component, a number of additional variance components, such as spousal correlation, sibship, household and maternal effects, may have strong contributions to inter-individual phenotype variation. In humans, the confounding effects of these components on heritability have not been studied thoroughly. We sought to obtain unbiased heritability estimates for complex traits in the presence of multiple variance components and also to estimate the contributions of these variance components to complex traits. We compared regression and variance component methods to estimate heritability in simulations when additional variance components existed. We then revisited heritability for several traits in Framingham Heart Study (FHS) participants. Using simulations, we found that failure to account for or misclassification of necessary variance components yielded biased heritability estimates. The direction and magnitude of the bias varied depending on a variance structure and an estimation method. Using the best fitted models to account for necessary variance components, we found that heritability estimates for most FHS traits were overestimated, ranging from 4 to 47 %, when we compared models that considered necessary variance components to models that only considered familial relationships. Spousal correlation explained 14-36 % of phenotypic variation in several anthropometric and lifestyle traits. Maternal and sibling effects also contributed to phenotypic variation, ranging from 3 to 5 % and 4 to 7 %, respectively, in several anthropometric and metabolic traits. Our findings may explain, in part, the missing heritability for some traits. C1 [Liu, Chunyu; Dupuis, Josee; Larson, Martin G.; Cupples, L. Adrienne; Vasan, Ramachandran S.; Levy, Daniel] Framingham Heart Dis Epidemiol Study, Framingham, MA 01702 USA. [Liu, Chunyu; Levy, Daniel] NHLBI, Populat Sci Branch, Div Intramural Res, NIH, Bethesda, MD 20892 USA. [Dupuis, Josee; Larson, Martin G.; Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA. [Larson, Martin G.] Boston Univ, Dept Math & Stat, Boston, MA 02215 USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA HNRCA, Nutr & Genom Lab, Boston, MA 02111 USA. [Vasan, Ramachandran S.] Boston Univ, Dept Med, Boston, MA 02118 USA. [Meigs, James B.] Massachusetts Gen Hosp, Div Gen Med, Boston, MA 02114 USA. [Meigs, James B.] Harvard Univ, Sch Med, Boston, MA 02114 USA. [Jacques, Paul F.] Tufts Univ, Jean Mayer USDA HNRCA, Nutr Epidemiol Program, Boston, MA 02111 USA. RP Liu, CY (reprint author), Framingham Heart Dis Epidemiol Study, Framingham, MA 01702 USA. EM liuchunyu2002@yahoo.com OI Ramachandran, Vasan/0000-0001-7357-5970 FU NHLBI, Framingham Heart Study, (NHLBI/NIH) [N01-HC-25195]; NIH NIDDK [R01 DK078616, K24 DK080140]; Boston University School of Medicine; US Department of Agriculture, Agriculture Research Service [53-K06-5-10, 58-1950-9-001] FX We acknowledge support from NHLBI, Framingham Heart Study, (NHLBI/NIH Contract #N01-HC-25195), from NIH NIDDK R01 DK078616 and K24 DK080140, from the Boston University School of Medicine, and from contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture, Agriculture Research Service. We thank Dr. Qiong Yang of Biostatistics, School of Public Health at Boston University for helpful discussions and Dr. Kathryn Lunetta of Biostatistics, School of Public Health at Boston University for providing R codes for incorporating additional variance components in lmekin () function. NR 41 TC 2 Z9 2 U1 4 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0340-6717 EI 1432-1203 J9 HUM GENET JI Hum. Genet. PD FEB PY 2015 VL 134 IS 2 BP 169 EP 179 DI 10.1007/s00439-014-1505-6 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA AZ3TM UT WOS:000348149500004 PM 25381465 ER PT J AU Eggleston, G DeLucca, A Sklanka, S Dalley, C Cyr, ES Powell, R AF Eggleston, Gillian DeLucca, Anthony Sklanka, Scottie Dalley, Caleb Cyr, Eldwin St. Powell, Randall TI Investigation of the stabilization and preservation of sweet sorghum juices SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Sweet sorghum juice; UV irradiation; Juice preservation; Clarification; Pasteurization; Microbial inactivation ID BICOLOR L. MOENCH; PILOT-PLANT; SUGAR-CANE; CLARIFICATION; FERMENTATION; DETERIORATION; MANUFACTURE; FEEDSTOCKS; MANNITOL; STARCH AB Sweet sorghum juice is extremely vulnerable to microbial spoilage during storage because of its high water activity and rich sugar medium, and this represents a major technical challenge. The effects of clarification (80 degrees C; limed to pH 6.5; 5 ppm polyanionic flocculant) and UV-C irradiation were investigated as stabilization and preservation treatments for juices stored at ambient temperature (similar to 25 degrees C). Juices were extracted by roller press from various sweet sorghum cultivars grown in humid and dry environments in Louisiana and Tennessee, respectively. Raw juices contained up to 109 total bacteria cfu/mL. Initial results indicated that pilot plant clarified juice was considerably more stable than raw or UV-C irradiated (15W lamp aquaculture system at similar to 25 degrees C) juice, irrespective of cultivar. Further experiments were undertaken to elucidate if heating (80 degrees C; 30 min) or impurity precipitation or both of these components of the clarification process were responsible for improved juice stability. Clarification or heating both achieved 3- to 4-log reductions of lactic acid bacteria in juices to negligible levels (<150 cfu/mL), and also significantly (P<0.05) reduced total bacterial counts. Juice heating gave similar results as the whole clarification process up to similar to 24 h storage, but became slightly worse between 24-28 h. Overall, clarified or heated (80 degrees C; 30 min) juice stored at 25 degrees C can be stored for at least 48 h before unacceptable spoilage occurs. Fingerprint ion chromatography with integrated pulsed amperometric detection (IC-IPAD) oligosaccharide profiles can be used to monitor sweet sorghum juice spoilage >100 cfu/mL lactic acid bacteria. Published by Elsevier B.V. C1 [Eggleston, Gillian; DeLucca, Anthony; Sklanka, Scottie; Cyr, Eldwin St.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Dalley, Caleb] USDA ARS, Sugarcane Unit, Houma, LA 70360 USA. [Powell, Randall] Delta BioRenewables LLC, Memphis, TN USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov FU United Sorghum Checkoff Program FX The authors thank Larry Boihem for help in obtaining sweet sorghum juice from Tennessee, and are grateful to the United Sorghum Checkoff Program for funding this research. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 6 Z9 6 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD FEB PY 2015 VL 64 BP 258 EP 270 DI 10.1016/j.indcrop.2014.09.008 PG 13 WC Agricultural Engineering; Agronomy SC Agriculture GA CA2OE UT WOS:000348746700032 ER PT J AU Larson, JE Sheley, RL Hardegree, SP Doescher, PS James, JJ AF Larson, Julie E. Sheley, Roger L. Hardegree, Stuart P. Doescher, Paul S. James, Jeremy J. TI Seed and seedling traits affecting critical life stage transitions and recruitment outcomes in dryland grasses SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE coleoptile tissue density; demography; emergence; functional traits; germination; hydrothermal time; path analysis; seed mass; seeding ID FUNCTIONAL TRAITS; PLANT TRAITS; REGENERATION NICHE; DEMOGRAPHIC RATES; GOOD PREDICTORS; SOIL-CONDITIONS; EMERGENCE; GERMINATION; GRASSLAND; SURVIVAL AB Seeding native plants is a key management practice to counter land degradation across the globe, yet the majority of seeding efforts fail, limiting our ability to accelerate ecosystem recovery. Recruitment requires transitions through several seed and seedling stages, some of which may have overriding influences on restoration outcomes. We lack, however, a general framework to understand and predict differences in these critical demographic processes across species. Functional traits influence fitness, and consequently, trait variation could provide the basis for a framework to explain and predict variation in life stage transition probabilities. We used seed and seedling traits, and field probabilities of germination, emergence, seedling establishment, and survival for 47 varieties of drylands grasses under two watering treatments to identify critical life stage transitions and quantify the effect of traits on cumulative survival through the first growing season. Variation in germination and emergence probabilities explained over 90% of the variation in cumulative survival regardless of seedling survival probabilities or watering treatment, with emergence probability being the strongest predictor of cumulative survival. Coleoptile tissue density and seed mass had significant effects on emergence and germination, respectively, explaining 10-23% of the variation in transition probabilities. Synthesis and applications. While the majority of functional trait work has centred on linking leaf and root traits to resource acquisition and utilization, our study demonstrates that traits associated with germination and emergence may have prevailing influences on restoration outcomes. A portion of these traits have been examined, but there is substantial opportunity to identify other key traits driving these demographic processes. These advancements will underpin our ability to develop trait-based frameworks for overcoming recruitment barriers and facilitating recovery of degraded systems across the globe. While the majority of functional trait work has centred on linking leaf and root traits to resource acquisition and utilization, our study demonstrates that traits associated with germination and emergence may have prevailing influences on restoration outcomes. A portion of these traits have been examined, but there is substantial opportunity to identify other key traits driving these demographic processes. These advancements will underpin our ability to develop trait-based frameworks for overcoming recruitment barriers and facilitating recovery of degraded systems across the globe. C1 [Larson, Julie E.] Oregon State Univ, Environm Sci Grad Program, Corvallis, OR 97330 USA. [Sheley, Roger L.] ARS, USDA, Burns, OR 97720 USA. [Hardegree, Stuart P.] ARS, USDA, Boise, ID 83712 USA. [Doescher, Paul S.] Oregon State Univ, Coll Forestry, Corvallis, OR 97330 USA. [James, Jeremy J.] Univ Calif, Sierra Foothills Res & Extens Ctr, Div Agr & Nat Resources, Browns Valley, CA 95918 USA. RP Larson, JE (reprint author), Chapman Univ, Schmid Coll Sci & Technol, Orange, CA 92866 USA. EM jlarson@chapman.edu FU USDA-Agricultural Research Service Area-wide EBIPM program FX We thank L. Ziegenhagen, B. Bingham, L. Smith, A. Boehm and L. Kosberg for assistance with data collection and E. Sabry, A. Muldoon, T. Jones and USDA Plant Materials personnel for providing resources and feedback on key project aspects. This study was funded as part of the USDA-Agricultural Research Service Area-wide EBIPM program. NR 61 TC 12 Z9 12 U1 10 U2 84 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD FEB PY 2015 VL 52 IS 1 BP 199 EP 209 DI 10.1111/1365-2664.12350 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA2LU UT WOS:000348740800022 ER PT J AU Johnson, DJ Flory, SL Shelton, A Huebner, C Clay, K AF Johnson, Daniel J. Flory, S. Luke Shelton, Angela Huebner, Cynthia Clay, Keith TI Interactive effects of a non-native invasive grass Microstegium vimineum and herbivore exclusion on experimental tree regeneration under differing forest management SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE competition; forest management; herbivores; invasion ecology; native-non-native interactions; species interactions; tree seedlings ID EXOTIC PLANT INVASIONS; WHITE-TAILED DEER; MEDIATED APPARENT COMPETITION; ENEMY RELEASE HYPOTHESIS; TRIN. A. CAMUS; HARDWOOD FOREST; JAPANESE STILTGRASS; DECIDUOUS FORESTS; SHADE-TOLERANT; UNITED-STATES AB Invasive plants, herbivores and site management history can play crucial roles in determining plant community composition. The net effects of invasive species on plant communities are well known, but we have a poor understanding of the relative contributions of direct competitive effects of invasive species and their interactions with herbivores and management practices. Understanding interactions among plant invasions, herbivores and management history is critical for predicting and managing long-term ecological effects of invasions on native communities. We investigated the effects of the invasive annual grass Microstegium vimineum, vertebrate herbivory, site management history and their interactions on seedling performance of five native tree species in six forest sites. Half of the sites had been subjected to recent timber harvesting, and half had not been managed for timber for at least 20years. We evaluated tree seedling survival and biomass after two growing seasons in blocked split-plots where the invasive grass was present or experimentally removed and where vertebrate herbivores were excluded by fencing or allowed onto the plots. Removal of the invasive grass and herbivore exclosures both had positive effects on seedling survival. Survival of all species was significantly increased by removal or by the interaction of removal with exclosure or timber harvest history. There was a significant interaction between exclosure and removal on seedling biomass where seedlings were larger in exclosure treatments with the invasive removed but smaller in exclosure treatments when the invader was present. Mean seedling biomass was 796% greater in harvested sites than in non-harvested sites. The significant positive effects of removal and recent timber harvest on the biomass of two Quercus species suggest strong impacts of invasion and management history on this important genus.Synthesis and applications. An invasive grass directly inhibited tree seedling performance but effects depended on herbivore exclusion treatment, timber harvest history and tree species. Our results suggest that forest management strategies should incorporate invasive plant control given that regeneration of desirable tree species can be influenced by plant invasions and their interactions with herbivores and previous management practices. An invasive grass directly inhibited tree seedling performance but effects depended on herbivore exclusion treatment, timber harvest history and tree species. Our results suggest that forest management strategies should incorporate invasive plant control given that regeneration of desirable tree species can be influenced by plant invasions and their interactions with herbivores and previous management practices. C1 [Johnson, Daniel J.; Shelton, Angela; Clay, Keith] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA. [Flory, S. Luke] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Huebner, Cynthia] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. RP Johnson, DJ (reprint author), Yale Univ, Sch Forestry & Environm Studies, New Haven, CT USA. EM dj4@indiana.edu FU Northern Research Station, USDA Forest Service [07-JV-11242300-083] FX Funding for the project came from the Northern Research Station, USDA Forest Service Joint Venture Agreement (#07-JV-11242300-083) to Indiana University, and access to research sites was provided by the Indiana Department of Natural Resources and the Indiana University Research and Teaching Preserve. Helpful comments and suggestions were provided by two anonymous reviewers. Field assistance was provided by C. Cleric, B. Goodale, J. Ferguson, M. Joyner, E. Menigat and I. Mason. NR 81 TC 2 Z9 3 U1 6 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD FEB PY 2015 VL 52 IS 1 BP 210 EP 219 DI 10.1111/1365-2664.12356 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA2LU UT WOS:000348740800023 ER PT J AU Yu, LJ Zhong, SY Bian, XD Heilman, WE AF Yu, Lejiang Zhong, Shiyuan Bian, Xindi Heilman, Warren E. TI Temporal and Spatial Variability of Wind Resources in the United States as Derived from the Climate Forecast System Reanalysis SO JOURNAL OF CLIMATE LA English DT Article ID ERA-INTERIM; EXTENDED RECONSTRUCTION; GEOPOTENTIAL HEIGHT; POWER RESOURCES; NCEP-CFSR; IMPACTS; OSCILLATION; SPEED; US; DISTRIBUTIONS AB This study examines the spatial and temporal variability of wind speed at 80 m above ground (the average hub height of most modern wind turbines) in the contiguous United States using Climate Forecast System Reanalysis (CFSR) data from 1979 to 2011. The mean 80-m wind exhibits strong seasonality and large spatial variability, with higher (lower) wind speeds in the winter (summer), and higher (lower) speeds over much of the Midwest and U.S. Northeast (U.S. West and Southeast). Trends are also variable spatially, with more upward trends in areas of the Great Plains and Intermountain West of the United States and more downward trends elsewhere. The leading EOF mode, which accounts for 20% (summer) to 33% (winter) of the total variance and represents in-phase variations across the United States, responds mainly to the North Atlantic Oscillation (NAO) in summer and El Nino Southern Oscillation (ENS 0) in the other seasons. The dominant variation pattern can be explained by a southerly/southwesterly (westerly) anomaly over the U.S. East (U.S. West) as a result of the anomalous mean sea level pressure (MSLP) pattern. The second EOF mode, which explains about 15% of the total variance and shows a seesaw pattern, is mainly related to the springtime Arctic Oscillation (AO), the summertime recurrent circumglobal teleconnection (CGT), the autumn Pacific decadal oscillation (PDO), and the winter El Nino Modoki. The anomalous jet stream and MSLP patterns associated with these indices are responsible for the wind variation. C1 [Yu, Lejiang] Nanjing Univ Informat Sci & Technol, Appl Hydrometeorol Res Inst, Nanjing, Jiangsu, Peoples R China. [Yu, Lejiang; Zhong, Shiyuan] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. [Yu, Lejiang; Zhong, Shiyuan] Michigan State Univ, Ctr Global Change & Earth Observat, E Lansing, MI 48824 USA. [Bian, Xindi; Heilman, Warren E.] US Forest Serv, No Res Stn, USDA, Lansing, MI USA. RP Zhong, SY (reprint author), Michigan State Univ, Dept Geog, 673 Auditorium Rd, E Lansing, MI 48824 USA. EM zhongs@msu.edu FU USDA Forest Service Northern Research Station [11-JV-11242306-065]; AgBioResearch of Michigan State University FX The authors thank the efforts made by NCEP in producing CFSR data. This research is supported by the USDA Forest Service Northern Research Station under Research Joint Venture Agreement 11-JV-11242306-065 and by AgBioResearch of Michigan State University. NR 58 TC 2 Z9 2 U1 0 U2 7 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0894-8755 EI 1520-0442 J9 J CLIMATE JI J. Clim. PD FEB 1 PY 2015 VL 28 IS 3 BP 1166 EP 1183 DI 10.1175/JCLI-D-14-00322.1 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CA9WJ UT WOS:000349275200015 ER PT J AU Otkin, JA Anderson, MC Hain, C Svoboda, M AF Otkin, Jason A. Anderson, Martha C. Hain, Christopher Svoboda, Mark TI Using Temporal Changes in Drought Indices to Generate Probabilistic Drought Intensification Forecasts SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID EVAPORATIVE STRESS INDEX; EASTERN UNITED-STATES; METEOROLOGICAL DROUGHT; SURFACE-TEMPERATURE; CLIMATE-CHANGE; SOIL-MOISTURE; GREAT-PLAINS; CROP-YIELD; MODEL; PREDICTION AB In this study, the potential utility of using rapid temporal changes in drought indices to provide early warning of an elevated risk for drought development over subseasonal time scales is assessed. Standardized change anomalies were computed each week during the 2000-13 growing seasons for drought indices depicting anomalies in evapotranspiration, precipitation, and soil moisture. A rapid change index (RCI) that encapsulates the accumulated magnitude of rapid changes in the weekly anomalies was computed each week for each drought index, and then a simple statistical method was used to convert the RCI values into drought intensification probabilities depicting the likelihood that drought severity as analyzed by the U.S. Drought Monitor (USDM) would worsen in subsequent weeks. Local and regional case study analyses revealed that elevated drought intensification probabilities often occur several weeks prior to changes in the USDM and in topsoil moisture and crop condition datasets compiled by the National Agricultural Statistics Service. Statistical analyses showed that the RCI-derived probabilities are most reliable and skillful over the central and eastern United States in regions most susceptible to rapid drought development. Taken together, these results suggest that tools used to identify areas experiencing rapid changes in drought indices may be useful components of future drought early warning systems. C1 [Otkin, Jason A.] Univ Wisconsin, Cooperat Inst Meteorol Satellite Studies, Madison, WI 53706 USA. [Anderson, Martha C.] USDA, ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Hain, Christopher] Univ Maryland, Earth Syst Interdisciplinary Ctr, College Pk, MD 20742 USA. [Svoboda, Mark] Univ Nebraska, Natl Drought Mitigat Ctr, Lincoln, NE USA. RP Otkin, JA (reprint author), Univ Wisconsin, Cooperat Inst Meteorol Satellite Studies, 1225 W Dayton St, Madison, WI 53706 USA. EM jason.otkin@ssec.wisc.edu RI Otkin, Jason/D-1737-2012; Anderson, Martha/C-1720-2015 OI Otkin, Jason/0000-0003-4034-7845; Anderson, Martha/0000-0003-0748-5525 FU NOAA Climate Program Office's Sectoral Applications Research Program (SARP); NOAA Climate Program Office's Modeling, Analysis, Predictions, and Projections (MAPP) program [NA13OAR4310122, GC09-236] FX This work was supported by funds provided by the NOAA Climate Program Office's Sectoral Applications Research Program (SARP) and the Modeling, Analysis, Predictions, and Projections (MAPP) program under Grants NA13OAR4310122 and GC09-236. NR 72 TC 5 Z9 5 U1 2 U2 20 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD FEB PY 2015 VL 16 IS 1 BP 88 EP 105 DI 10.1175/JHM-D-14-0064.1 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CB1DR UT WOS:000349367500007 ER PT J AU Hottenstein, JD Ponce-Campos, GE Moguel-Yanes, J Moran, MS AF Hottenstein, John D. Ponce-Campos, Guillermo E. Moguel-Yanes, Julio Moran, M. Susan TI Impact of Varying Storm Intensity and Consecutive Dry Days on Grassland Soil Moisture SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID CLIMATE-CHANGE; PRECIPITATION PATTERNS; EVENT SIZE; WATER; RESPONSES; MISSION; PRODUCTIVITY; EXTREMES; DROUGHT; REGIMES AB Intra-annual precipitation patterns are expected to shift toward more intense storms and longer dry periods because of changes in climate within future decades. Using satellite-derived estimates of plant growth combined with in situ measurements of precipitation and soil moisture between 1999 and 2013, this study quantified the relationship between intra-annual precipitation patterns, annual average soil moisture (at 5-cm depth), and plant growth at nine grassland sites across the southern United States. Results showed a fundamental difference in the response to varying precipitation patterns between mesic and semiarid grasslands. Surface soil moisture in mesic grasslands decreased with an increase of high-intensity storms, whereas in semiarid grasslands, soil moisture decreased with longer dry periods. For these sites, annual average soil moisture was a better indicator of grassland production than total annual precipitation. This improved ability to predict variability in soil moisture and plant growth with changing hydroclimatic conditions will result in more efficient resource management and better-informed policy decisions. C1 [Hottenstein, John D.] Univ Arizona, Tucson, AZ USA. [Ponce-Campos, Guillermo E.; Moran, M. Susan] USDA, ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Ponce-Campos, Guillermo E.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ USA. [Moguel-Yanes, Julio] Univ Juarez Autonoma Tabasco, Villahermosa, Tabasco, Mexico. RP Moran, MS (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM susan.moran@ars.usda.gov FU University of Arizona/Arizona NASA Space Grant Consortium; NASA SMAP Science Definition Team [08-SMAPSDT08-0042]; University of Arizona Graduate College Latin America Summer Research Program FX The research was funded and supported by the University of Arizona/Arizona NASA Space Grant Consortium, the NASA SMAP Science Definition Team under Agreement 08-SMAPSDT08-0042, and the University of Arizona Graduate College Latin America Summer Research Program. The SMAP Early Adopter Program provided valuable technical support. We acknowledge the U.S. Department of Energy ARM Climate Research Facility and the USDA SCAN network for making these data available. We would also like to thank Dr. Joe Watkins, University of Arizona Department of Mathematics, for his help in the statistical analysis of our results. NR 35 TC 2 Z9 2 U1 3 U2 27 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD FEB PY 2015 VL 16 IS 1 BP 106 EP 117 DI 10.1175/JHM-D-14-0057.1 PG 12 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CB1DR UT WOS:000349367500008 ER PT J AU Moran, MS Doorn, B Escobar, V Brown, ME AF Moran, M. Susan Doorn, Bradley Escobar, Vanessa Brown, Molly E. TI Connecting NASA Science and Engineering with Earth Science Applications SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID SOIL-MOISTURE DATA; PASSIVE SMAP MISSION; ASSIMILATION; SATELLITE; RAINFALL; DROUGHT AB The National Research Council (NRC) recently highlighted the dual role of NASA to support both science and applications in planning Earth observations. This article reports the efforts of the NASA Applied Sciences Program and NASA Soil Moisture Active Passive (SMAP) mission to integrate applications with science and engineering in prelaunch planning. The SMAP Early Adopter program supported the prelaunch applied research that comprises the SMAP Special Collection of the Journal of Hydrometeorology. This research, in turn, has resulted in unprecedented prelaunch preparation for SMAP applications and critical feedback to the mission to improve product specifications and distribution for postlaunch applications. These efforts have been a learning experience that should provide direction for upcoming missions and set some context for the next NRC decadal survey. C1 [Moran, M. Susan] USDA, ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Doorn, Bradley] NASA, Appl Sci Program, Washington, DC 20546 USA. [Escobar, Vanessa] NASA, Goddard Space Flight Ctr, Sigma Space Corp, Greenbelt, MD 20771 USA. [Brown, Molly E.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. RP Moran, MS (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM susan.moran@ars.usda.gov RI Brown, Molly/E-2724-2010 OI Brown, Molly/0000-0001-7384-3314 FU NASA [08-SMAPSDT08-0042] FX This work has been supported in part by NASA (08-SMAPSDT08-0042), and many ideas have been clarified though discussions with the NASA ASP Applied Sciences Advisory Group (ASAG). The SMAP Early Adopter program was a mission-wide effort by the SMAP project, NASA Headquarters, and the SMAP Science Team. NR 21 TC 2 Z9 2 U1 1 U2 11 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD FEB PY 2015 VL 16 IS 1 BP 473 EP 483 DI 10.1175/JHM-D-14-0093.1 PG 11 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CB1DR UT WOS:000349367500032 ER PT J AU Claycombe, KJ Roemmich, JN Johnson, L Vomhof-DeKrey, EE Johnson, WT AF Claycombe, Kate J. Roemmich, James N. Johnson, LuAnn Vomhof-DeKrey, Emilie E. Johnson, W. Thomas TI Skeletal muscle Sirt3 expression and mitochondrial respiration are regulated by a prenatal low-protein diet SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Maternal low-protein diet; Skeletal muscle respiration; Mitochondria; SIRT3; SDH ID INSULIN-RESISTANCE; DIABETES-MELLITUS; LYSINE ACETYLATION; METABOLIC SYNDROME; DYSFUNCTION; DEACETYLASE; GROWTH; RAT; INFILTRATION; CONSEQUENCE AB Malnutrition during the fetal growth period increases risk for later obesity and type 2 diabetes mellitus (T2DM). We have shown that a prenatal low-protein (8% protein; LP) diet followed by postnatal high-fat (45% fat; HF) diet results in offspring propensity for adipose tissue catch-up growth, obesity and T2DM in Sprague-Dawley rats. Skeletal muscle is the major tissue for insulin-mediated glucose uptake. Dysfunctional skeletal muscle mitochondrial function, particularly reduction in expression of mitochondrial protein sirtuin protein 3 (Sirt3) contributes to development of T2DM by reducing mitochondrial respiration. Therefore, we hypothesized that maternal LP and postnatal HF diets would increase T2DM risk due to Sirt3 dysfunction within skeletal muscle mitochondria. Using our maternal LP and postnatal HF diet model, we showed that skeletal muscle mitochondrial oxygen consumption rate was decreased by maternal LP diet. Mitochondria copy number, mitochondrial thermogenesis (UCP-1) expression and mitochondrial biogenic factors including nuclear respiratory factor 1 and cytochrome c oxidases 1 and 4 were unaffected by maternal LP and postnatal HF diets. Skeletal muscle Sirt3 mRNA decreased with maternal LP diet. A mitochondrial substrate of Sirt3, succinate dehydrogenase (SDH), is regulated by Sirt3 via lysine residue acetylation status of SDH. Acetylated SDH protein (inactive form) levels were moderately decreased by maternal LP diet. Taken together, these data suggest that maternal LP and postnatal HF diets may increase the risk for T2D by decreasing skeletal muscle oxidative respiration via increased Sirt3 and possibly by decreased amounts of the active form of SDH enzyme. Published by Elsevier Inc. C1 [Claycombe, Kate J.; Roemmich, James N.; Johnson, LuAnn; Vomhof-DeKrey, Emilie E.; Johnson, W. Thomas] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. RP Claycombe, KJ (reprint author), Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. EM kate.claycombe@ars.usda.gov FU USDA Agricultural Research Service Project [5450-51000-047-00D] FX This work was supported by USDA Agricultural Research Service Project No. 5450-51000-047-00D. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 39 TC 7 Z9 7 U1 0 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD FEB PY 2015 VL 26 IS 2 BP 184 EP 189 DI 10.1016/j.jnutbio.2014.10.003 PG 6 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA CB4DL UT WOS:000349578300010 PM 25483313 ER PT J AU McLellan, E Robertson, D Schilling, K Tomer, M Kostel, J Smith, D King, K AF McLellan, Eileen Robertson, Dale Schilling, Keith Tomer, Mark Kostel, Jill Smith, Doug King, Kevin TI REDUCING NITROGEN EXPORT FROM THE CORN BELT TO THE GULF OF MEXICO: AGRICULTURAL STRATEGIES FOR REMEDIATING HYPOXIA SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE watershed management; nutrients; best management practices (BMPs); non-point source pollution; eutrophication; rivers; streams; wetlands ID MISSISSIPPI/ATCHAFALAYA RIVER-BASIN; WATER-QUALITY IMPROVEMENT; RIPARIAN FOREST; NITRATE LOSS; MISSISSIPPI RIVER; TILE DRAINAGE; CONSERVATION PRACTICES; CONSTRUCTED WETLANDS; SUBSURFACE DRAINAGE; NUTRIENT TRANSPORT AB SPAtially Referenced Regression on Watershed models developed for the Upper Midwest were used to help evaluate the nitrogen-load reductions likely to be achieved by a variety of agricultural conservation practices in the Upper Mississippi-Ohio River Basin (UMORB) and to compare these reductions to the 45% nitrogen-load reduction proposed to remediate hypoxia in the Gulf of Mexico (GoM). Our results indicate that nitrogen-management practices (improved fertilizer management and cover crops) fall short of achieving this goal, even if adopted on all cropland in the region. The goal of a 45% decrease in loads to the GoM can only be achieved through the coupling of nitrogen-management practices with innovative nitrogen-removal practices such as tile-drainage treatment wetlands, drainage-ditch enhancements, stream-channel restoration, and floodplain reconnection. Combining nitrogen-management practices with nitrogen-removal practices can dramatically reduce nutrient export from agricultural landscapes while minimizing impacts to agricultural production. With this approach, it may be possible to meet the 45% nutrient reduction goal while converting less than 1% of cropland in the UMORB to nitrogen-removal practices. Conservationists, policy makers, and agricultural producers seeking a workable strategy to reduce nitrogen export from the Corn Belt will need to consider a combination of nitrogen-management practices at the field scale and diverse nitrogen-removal practices at the landscape scale. C1 [McLellan, Eileen] Environm Def Fund, Washington, DC 20009 USA. [Robertson, Dale] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI 53562 USA. [Schilling, Keith] Univ Iowa, IIHR Hydrosci & Engn, Iowa City, IA 52242 USA. [Tomer, Mark] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Kostel, Jill] Wetlands Initiat, Chicago, IL 60604 USA. [Smith, Doug] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47906 USA. [King, Kevin] USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. RP McLellan, E (reprint author), Environm Def Fund, 1875 Connecticut Ave, Washington, DC 20009 USA. EM emclellan@edf.org OI Robertson, Dale/0000-0001-6799-0596 FU Keith Campbell Foundation; McKnight Foundation; Walton Family Foundation FX We would like to thank Ralph Heimlich of Agricultural Conservation Economics for analyzing the various conservation scenarios, and gratefully acknowledge the support by the Keith Campbell Foundation, the McKnight Foundation, and the Walton Family Foundation. Dave Saad, Lisa Schulte Moore, Dave Kovacic, and Chris Craft offered helpful comments on an earlier draft of the manuscript. Finally, we appreciate the efforts of the three anonymous reviewers whose constructive criticism greatly improved the manuscript. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 81 TC 6 Z9 6 U1 11 U2 88 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD FEB PY 2015 VL 51 IS 1 BP 263 EP 289 DI 10.1111/jawr.12246 PG 27 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA CA8FF UT WOS:000349151900019 ER PT J AU Huang, YF Guo, LY Qiu, J Chen, X Hu-Li, J Siebenlist, U Williamson, PR Urban, JF Paul, WE AF Huang, Yuefeng Guo, Liying Qiu, Jin Chen, Xi Hu-Li, Jane Siebenlist, Ulrich Williamson, Peter R. Urban, Joseph F. Paul, William E. TI IL-25-responsive, lineage-negative KLRG1(hi) cells are multipotential 'inflammatory' type 2 innate lymphoid cells SO NATURE IMMUNOLOGY LA English DT Article ID TRANSCRIPTION FACTOR GATA3; LUNG INFLAMMATION; IMMUNITY; T(H)2; HOMEOSTASIS; POPULATION; INFECTION; ELICITS; IL-25; FATE AB Innate lymphoid cells (ILCs) are lymphocyte-like cells that lack T cell or B cell antigen receptors and mediate protective and repair functions through cytokine secretion. Among these, type 2 ILCs (ILC2 cells) are able to produce type 2 cytokines. We report the existence of an inflammatory ILC2 (iILC2) population responsive to interleukin 25 (IL-25) that complemented IL-33-responsive natural ILC2 (nILC2) cells. iILC2 cells developed into nILC2-like cells in vitro and in vivo and contributed to the expulsion of Nippostrongylus brasiliensis. They also acquired IL-17-producing ability and provided partial protection against Candida albicans. We propose that iILC2 cells are transient progenitors of ILCs mobilized by inflammation and infection that develop into nILC2-like cells or ILC3-like cells and contribute to immunity to both helminths and fungi. C1 [Huang, Yuefeng; Guo, Liying; Chen, Xi; Hu-Li, Jane; Paul, William E.] NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. [Qiu, Jin; Williamson, Peter R.] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Siebenlist, Ulrich] NIAID, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. [Urban, Joseph F.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. RP Paul, WE (reprint author), NIAID, Immunol Lab, NIH, Bldg 10, Bethesda, MD 20892 USA. EM wpaul@niaid.nih.gov OI Urban, Joseph/0000-0002-1590-8869 FU NIAID Division of Intramural Research (US National Institutes of Health) FX We thank J. Zhu for critical reading of the manuscript; J. Edwards for assistance in the preparation of sorter-purified cells; L. Feigenbaum of the SAIC Laboratory Animal Sciences Program for injection of the recombinant bacterial artificial chromosome into oocytes, transfer into pseudopregnant females and screening of pups; C. Dong (MD Anderson Cancer Center) for IL-17F-RFP reporter mice; A. McKenzie (MRC Laboratory of Molecular Biology, Cambridge) for Il1rl1-/- mice; and members of Laboratory of Immunology at NIAID for discussions. Supported by the NIAID Division of Intramural Research (US National Institutes of Health). NR 29 TC 63 Z9 64 U1 3 U2 16 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1529-2908 EI 1529-2916 J9 NAT IMMUNOL JI Nat. Immunol. PD FEB PY 2015 VL 16 IS 2 BP 161 EP + DI 10.1038/ni.3078 PG 10 WC Immunology SC Immunology GA AZ3QZ UT WOS:000348143100008 PM 25531830 ER PT J AU Wang, X Ort, DR Yuan, JS AF Wang, Xin Ort, Donald R. Yuan, Joshua S. TI Photosynthetic terpene hydrocarbon production for fuels and chemicals SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Review DE photosynthesis; terpenoid; advanced biofuel; hydrocarbon; MEP ID MEVALONATE-INDEPENDENT PATHWAY; METHYLERYTHRITOL 4-PHOSPHATE PATHWAY; PLASTIDIC ISOPRENOID BIOSYNTHESIS; DEOXYXYLULOSE PHOSPHATE-PATHWAY; ESCHERICHIA-COLI; BIOFUELS PRODUCTION; ENGINEERING CYANOBACTERIA; HETEROLOGOUS EXPRESSION; MICROBIAL-PRODUCTION; GLANDULAR TRICHOMES AB Photosynthetic hydrocarbon production bypasses the traditional biomass hydrolysis process and represents the most direct conversion of sunlight energy into the next-generation biofuels. As a major class of biologically derived hydrocarbons with diverse structures, terpenes are also valuable in producing a variety of fungible bioproducts in addition to the advanced drop-in' biofuels. However, it is highly challenging to achieve the efficient redirection of photosynthetic carbon and reductant into terpene biosynthesis. In this review, we discuss four major scientific and technical barriers for photosynthetic terpene production and recent advances to address these constraints. Collectively, photosynthetic terpene production needs to be optimized in a systematic fashion, in which the photosynthesis improvement, the optimization of terpene biosynthesis pathway, the improvement of key enzymes and the enhancement of sink effect through terpene storage or secretion are all important. New advances in synthetic biology also offer a suite of potential tools to design and engineer photosynthetic terpene platforms. The systemic integration of these solutions may lead to disruptive' technologies to enable biofuels and bioproducts with high efficiency, yield and infrastructure compatibility. C1 [Wang, Xin; Yuan, Joshua S.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Wang, Xin; Yuan, Joshua S.] Texas A&M Univ, College Stn, TX USA. [Wang, Xin; Yuan, Joshua S.] Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX USA. [Ort, Donald R.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL USA. [Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. RP Yuan, JS (reprint author), Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. EM syuan@tamu.edu RI Wang, Xin/B-6258-2015 FU U.S. Department of Energy, ARPA-E grant FX This study was supported by the U.S. Department of Energy, ARPA-E grant to Joshua S. Yuan, Don R. Ort and other Co-PIs. We thank Dr. Hong Ma at Texas A&M AgriLife for good discussions. NR 122 TC 7 Z9 7 U1 7 U2 50 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD FEB PY 2015 VL 13 IS 2 BP 137 EP 146 DI 10.1111/pbi.12343 PG 10 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CA8JE UT WOS:000349164100001 PM 25626473 ER PT J AU Yargicoglu, EN Sadasivam, BY Reddy, KR Spokas, K AF Yargicoglu, Erin N. Sadasivam, Bala Yamini Reddy, Krishna R. Spokas, Kurt TI Physical and chemical characterization of waste wood derived biochars SO WASTE MANAGEMENT LA English DT Article DE Biochar; Biomass pyrolysis; Carbon sequestration; PAHs; Heavy metals; Waste wood ID POLYCYCLIC AROMATIC-HYDROCARBONS; BLACK CARBON BIOCHAR; PYROLYSIS TEMPERATURE; MOLECULAR-STRUCTURE; SOIL AMENDMENT; HEAVY-METALS; SORPTION; BIOMASS; CHARCOAL; MECHANISMS AB Biochar, a solid byproduct generated during waste biomass pyrolysis or gasification in the absence (or near-absence) of oxygen, has recently garnered interest for both agricultural and environmental management purposes owing to its unique physicochemical properties. Favorable properties of biochar include its high surface area and porosity, and ability to adsorb a variety of compounds, including nutrients, organic contaminants, and some gases. Physical and chemical properties of biochars are dictated by the feedstock and production processes (pyrolysis or gasification temperature, conversion technology and pre- and post-treatment processes, if any), which vary widely across commercially produced biochars. In this study, several commercially available biochars derived from waste wood are characterized for physical and chemical properties that can signify their relevant environmental applications. Parameters characterized include: physical properties (particle size distribution, specific gravity, density, porosity, surface area), hydraulic properties (hydraulic conductivity and water holding capacity), and chemical and electrochemical properties (organic matter and organic carbon contents, pH, oxidation-reduction potential and electrical conductivity, zeta potential, carbon, nitrogen and hydrogen (CHN) elemental composition, polycyclic aromatic hydrocarbons (PAHs), heavy metals, and leachable PAHs and heavy metals). A wide range of fixed carbon (0-47.8%), volatile matter (28-74.1%), and ash contents (1.5-65.7%) were observed among tested biochars. A high variability in surface area (0.1-155.1 g/m(2)) and PAH and heavy metal contents of the solid phase among commercially available biochars was also observed (0.7-83 mg kg(-1)), underscoring the importance of pre-screening biochars prior to application. Production conditions appear to dictate PAM content - with the highest PAHs observed in biochar produced via fast pyrolysis and lowest among the gasification-produced biochars. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Yargicoglu, Erin N.; Sadasivam, Bala Yamini; Reddy, Krishna R.] Univ Illinois, Dept Civil & Mat Engn, Chicago, IL 60607 USA. [Spokas, Kurt] Univ Minnesota, USDA ARS, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Reddy, KR (reprint author), Univ Illinois, Dept Civil & Mat Engn, 842 West Taylor St, Chicago, IL 60607 USA. EM eyargi2@uic.edu; bsadas2@uic.edu; kreddy@uic.edu; kurt.spokas@ars.usda.gov RI Spokas, Kurt/F-4839-2016; OI Spokas, Kurt/0000-0002-5049-5959; Reddy, Krishna/0000-0002-6577-1151 FU U.S. National Science Foundation [1200799] FX The financial support for this project was provided by the U.S. National Science Foundation (Grant CMMI#1200799), which is gratefully acknowledged. NR 65 TC 22 Z9 23 U1 16 U2 157 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0956-053X J9 WASTE MANAGE JI Waste Manage. PD FEB PY 2015 VL 36 BP 256 EP 268 DI 10.1016/j.wasman.2014.10.029 PG 13 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CB3FG UT WOS:000349513000029 PM 25464942 ER PT J AU Ma, YY Smith, CE Lai, CQ Irvin, MR Parnell, LD Lee, YC Pham, L Aslibekyan, S Claas, SA Tsai, MY Borecki, IB Kabagambe, EK Berciano, S Ordovas, JM Absher, DM Arnett, DK AF Ma, Yiyi Smith, Caren E. Lai, Chao-Qiang Irvin, Marguerite R. Parnell, Laurence D. Lee, Yu-Chi Pham, Lucia Aslibekyan, Stella Claas, Steven A. Tsai, Michael Y. Borecki, Ingrid B. Kabagambe, Edmond K. Berciano, Silvia Ordovas, Jose M. Absher, Devin M. Arnett, Donna K. TI Genetic variants modify the effect of age on APOE methylation in the Genetics of Lipid Lowering Drugs and Diet Network study SO AGING CELL LA English DT Article DE apolipoprotein E; age; DNA methylation; variants; epidemiology; interaction ID CORONARY-HEART-DISEASE; APOLIPOPROTEIN-E GENE; DNA METHYLATION; ALZHEIMERS-DISEASE; MONOZYGOTIC TWINS; CPG SITES; POLYMORPHISM; PROMOTER; GENOME; ASSOCIATION AB Although apolipoprotein E (APOE) variants are associated with age-related diseases, the underlying mechanism is unknown and DNA methylation may be a potential one. With methylation data, measured by the Infinium Human Methylation 450 array, from 993 participants (age ranging from 18 to 87years) in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study, and from Encyclopedia of DNA Elements (ENCODE) consortium, combined with published methylation datasets, we described the methylation pattern of 13 CpG sites within APOE locus, their correlations with gene expression across cell types, and their relationships with age, plasma lipids, and sequence variants. Based on methylation levels and the genetic regions, we categorized the 13 APOE CpG sites into three groups: Group 1 showed hypermethylation (>50%) and were located in the promoter region, Group 2 exhibited hypomethylation (<50%) and were located in the first two exons and introns, and Group 3 showed hypermethylation (>50%) and were located in the exon 4. APOE methylation was negatively correlated with gene expression (minimum r=-0.66, P=0.004). APOE methylation was significantly associated with age (minimum P=2.06E-08) and plasma total cholesterol (minimum P=3.53E-03). Finally, APOE methylation patterns differed across APOE epsilon variants (minimum P=3.51E-05) and the promoter variant rs405509 (minimum P=0.01), which further showed a significant interaction with age (P=0.03). These findings suggest that methylation may be a potential mechanistic explanation for APOE functions related to aging and call for further molecular mechanistic studies. C1 [Ma, Yiyi; Smith, Caren E.; Lai, Chao-Qiang; Parnell, Laurence D.; Lee, Yu-Chi; Pham, Lucia; Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Irvin, Marguerite R.; Aslibekyan, Stella; Claas, Steven A.; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Kabagambe, Edmond K.] Vanderbilt Univ, Dept Med, Nashville, TN USA. [Berciano, Silvia; Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. [Ordovas, Jose M.] CNIC, Dept Epidemiol, Madrid, Spain. [Absher, Devin M.] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. RP Ma, YY (reprint author), Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM yiyi.ma@tufts.edu OI Claas, Steven/0000-0001-9789-8395 FU National Heart Lung and Blood Institute [U01HL072524-04, 5R01HL1043135-04]; National Institute of Neurological Disorders and Stroke [T32NS054584]; USDA [58-1950-0-014]; [K08 HL112845] FX This study is funded by National Heart Lung and Blood Institute Grant U01HL072524-04 and 5R01HL1043135-04, and the National Institute of Neurological Disorders and Stroke Grant T32NS054584. C. Smith is supported by K08 HL112845. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. This material is based upon work supported by the USDA, under agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 45 TC 8 Z9 8 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1474-9718 EI 1474-9726 J9 AGING CELL JI Aging Cell PD FEB PY 2015 VL 14 IS 1 BP 49 EP 59 DI 10.1111/acel.12293 PG 11 WC Cell Biology; Geriatrics & Gerontology SC Cell Biology; Geriatrics & Gerontology GA CA7KQ UT WOS:000349096600006 PM 25476875 ER PT J AU Gunn, KM Fausey, NR Shang, YH Shedekar, VS Ghane, E Wahl, MD Brown, LC AF Gunn, Kpoti M. Fausey, Norman R. Shang, Yuhui Shedekar, Vinayak S. Ghane, Ehsan Wahl, Mark D. Brown, Larry C. TI Subsurface drainage volume reduction with drainage water management: Case studies in Ohio, USA SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Discharge; Paired watershed; On-farm experiment; Linear mixed model; Tile drainage ID GULF-OF-MEXICO; UNITED-STATES; NITRATE LOSS; MISSISSIPPI RIVER; NITROGEN LOSSES; QUALITY; SYSTEMS; YIELD; STRATEGIES; LANDS AB Drainage water management (DWM) is promoted as an agricultural best management practice that reduces subsurface drainage volume and thereby the transport of soluble nutrients to streams. This study was conducted on private crop fields to quantify the effect of managed subsurface drainage on daily subsurface drainage volume, in poorly drained and somewhat poorly drained soils of northwest Ohio. A paired zone approach was used where a part of each field was conventional free draining and the other part was under drainage water management. At each site, comparison of median daily subsurface drainage volume from the two zones indicated that drainage water management was effective at reducing daily subsurface drainage volume. A linear mixed model procedure was applied to determine the percent reduction in daily subsurface drainage volume as a result of drainage water management. Using the paired dataset at each site, the model predicted the total daily subsurface drainage volume from the managed zone as a function of the observed total daily subsurface drainage volume from the conventional zone. The percent reduction of daily subsurface drainage volume varied from 40% to 100% depending on site. While the magnitude of the reduction of the daily subsurface drainage volume is site specific, the general expectation is that if DWM is instituted broadly and appropriately in northwest Ohio, mean daily subsurface drainage volume would lessen on an annual basis. Such reduction may eventually translate into a reduction in nutrient loads exported from farm fields. (C) 2014 Elsevier B.V. All rights reserved. C1 [Gunn, Kpoti M.; Shang, Yuhui; Shedekar, Vinayak S.; Ghane, Ehsan; Wahl, Mark D.; Brown, Larry C.] Ohio State Univ, Dept Food Agr & Biol Engn, Columbus, OH 43210 USA. [Fausey, Norman R.] USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. RP Gunn, KM (reprint author), Ohio State Univ, Dept Food Agr & Biol Engn, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM gunn.54@osu.edu FU USDA-NRCS Conservation Innovation grant through the Agricultural Drainage Management Coalition; USDA-NRCS State Conservation Innovation grant through the Maumee Valley RCD; Overholt Drainage Education and Research Program; Department of Food, Agricultural and Biological Engineering, Ohio Agricultural Research and Development Center, Ohio State University Extension, The Ohio State University; USDA-ARS Soil Drainage Research Unit (SDRU); DWM Task Force FX This project was funded, in part, by a USDA-NRCS Conservation Innovation grant through the Agricultural Drainage Management Coalition (http://admcoalition.com/); a USDA-NRCS State Conservation Innovation grant through the Maumee Valley RC & D; the Overholt Drainage Education and Research Program, Department of Food, Agricultural and Biological Engineering, Ohio Agricultural Research and Development Center, Ohio State University Extension, The Ohio State University; and collaboration with the USDA-ARS Soil Drainage Research Unit (SDRU), and in association with the DWM Task Force (http://hostedweb.cfaes. ohio-state.edu/usdasdru/AD1VIS/ADMSindex.htm). The authors thank the cooperating farmers for providing their farms as research and demonstration sites. We also express our gratitude to Ethan Nicol, Lindsay Kilpatrick, Keith Libben, Zachery Marlow, and other undergraduate students of the Department, and especially Phil Levison (USDA-ARS-SDRU), for vital assistance with data collection and processing. NR 41 TC 1 Z9 1 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD FEB PY 2015 VL 149 BP 131 EP 142 DI 10.1016/j.agwat.2014.10.014 PG 12 WC Agronomy; Water Resources SC Agriculture; Water Resources GA CA4SE UT WOS:000348893900012 ER PT J AU Gillespie, C Maalouf, J Yuan, KM Cogswell, ME Gunn, JP Levings, J Moshfegh, A Ahuja, JKC Merritt, R AF Gillespie, Cathleen Maalouf, Joyce Yuan, Keming Cogswell, Mary E. Gunn, Janelle P. Levings, Jessica Moshfegh, Alanna Ahuja, Jaspreet K. C. Merritt, Robert TI Sodium content in major brands of US packaged foods, 2009 SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE nutrition; packaged foods; sodium; diet; food ID PROCESSED FOODS; DIETARY-SODIUM; BLOOD-PRESSURE; UNITED-STATES; NEW-ZEALAND; DASH DIET; REDUCTION; PATTERNS; CONSUMPTION AB Background: Most Americans consume more sodium than is recommended, the vast majority of which comes from commercially packaged and restaurant foods. In 2010 the Institute of Medicine recommended that manufacturers reduce the amount of sodium in their products. Objective: The aim was to assess the sodium content in commercially packaged food products sold in US grocery stores in 2009. Design: With the use of sales and nutrition data from commercial sources, we created a database with nearly 8000 packaged food products sold in major US grocery stores in 2009. We estimated the sales-weighted mean and distribution of sodium content (mg/serving, mg/100 g, and mg/kcal) of foods within food groups that contribute the most dietary sodium to the US diet. We estimated the proportion of products within each category that exceed 1) the Food and Drug Administration's (FDA's) limits for sodium in foods that use a "healthy" label claim and 2) 1150 mg/serving or 50% of the maximum daily intake recommended in the 2010 Dietary Guidelines for Americans. Results: Products in the meat mixed dishes category had the highest mean and median sodium contents per serving (966 and 970 mg, respectively). Products in the salad dressing and vegetable oils category had the highest mean and median concentrations per 100 g (1072 and 1067 mg, respectively). Sodium density was highest in the soup category (18.4 mg/kcal). More than half of the products sold in 11 of the 20 food categories analyzed exceeded the FDA limits for products with a "healthy" label claim. In 4 categories, >10% of the products sold exceeded 1150 mg/serving. Conclusions: The sodium content in packaged foods sold in major US grocery stores varied widely, and a large proportion of top-selling products exceeded limits, indicating the potential for reduction. Ongoing monitoring is necessary to evaluate the progress in sodium reduction. C1 [Gillespie, Cathleen; Maalouf, Joyce; Yuan, Keming; Cogswell, Mary E.; Gunn, Janelle P.; Levings, Jessica; Merritt, Robert] CDC, Div Heart Dis & Stroke Prevent, Atlanta, GA 30333 USA. [Moshfegh, Alanna; Ahuja, Jaspreet K. C.] USDA ARS, Beltsville, MD USA. RP Gillespie, C (reprint author), Ctr Dis Control & Prevent, Mailstop F-72,4770 Buford Highway NE, Atlanta, GA 30341 USA. EM cgillespie@cdc.gov FU CDC FX Supported by the CDC. NR 27 TC 5 Z9 5 U1 6 U2 16 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 2015 VL 101 IS 2 BP 344 EP 353 DI 10.3945/ajcn.113.078980 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA5US UT WOS:000348973900014 PM 25646332 ER PT J AU Mercado, CI Cogswell, ME Valderrama, AL Wang, CY Loria, CM Moshfegh, AJ Rhodes, DG Carriquiry, AL AF Mercado, Carla I. Cogswell, Mary E. Valderrama, Amy L. Wang, Chia-Yih Loria, Catherine M. Moshfegh, Alanna J. Rhodes, Donna G. Carriquiry, Alicia L. TI Difference between 24-h diet recall and urine excretion for assessing population sodium and potassium intake in adults aged 18-39 y SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE biomarker; diet; sodium; urine; validation study ID CHRONIC KIDNEY-DISEASE; BLOOD-PRESSURE; H RECALLS; CARDIOVASCULAR RISK; RACIAL-DIFFERENCES; CALCIUM INTAKE; FOOD-INTAKE; VALIDATION; BALANCE; COLLECTION AB Background: Limited data are available on the accuracy of 24-h dietary recalls used to monitor US sodium and potassium intakes. Objective: We examined the difference in usual sodium and potassium intakes estimated from 24-h dietary recalls and urine collections. Design: We used data from a cross-sectional study in 402 participants aged 18-39 y (similar to 50% African American) in the Washington, DC, metropolitan area in 2011. We estimated means and percentiles of usual intakes of daily dietary sodium (dNa) and potassium (dK) and 24-h urine excretion of sodium (uNa) and potassium (uK). We examined Spearman's correlations and differences between estimates from dietary and urine measures. Multiple linear regressions were used to evaluate the factors associated with the difference between dietary and urine measures. Results: Mean differences between diet and urine estimates were higher in men [dNa uNa (95% CI) = 936.8 (787.1, 1086.5) mg/d and dK - uK = 571.3 (448.3, 694.3) mg/d] than in women [dNa uNa (95% CI) = 108.3 (11.1, 205.4) mg/d and dK uK = 163.4 (85.3, 241.5 mg/d)]. Percentile distributions of diet and urine estimates for sodium and potassium differed for men. Spearman's correlations between measures were 0.16 for men and 0.25 for women for sodium and 0.39 for men and 0.29 for women for potassium. Urinary creatinine, total caloric intake, and percentages of nutrient intake from mixed dishes were independently and consistently associated with the differences between diet and urine estimates of sodium and potassium intake. For men, body mass index was also associated. Race was associated with differences in estimates of potassium intake. Conclusions: Low correlations and differences between dietary and urinary sodium or potassium may be due to measurement error in one or both estimates. Future analyses using these methods to assess sodium and potassium intake in relation to health outcomes may consider stratifying by factors associated with the differences in estimates from these methods. This trial was registered at clinicaltrials.gov as NCT01631240. C1 [Mercado, Carla I.; Cogswell, Mary E.; Valderrama, Amy L.] Ctr Dis Control, Natl Ctr Chron Dis Prevent & Hlth Promot, Div Heart Dis & Stroke Prevent, Atlanta, GA 30333 USA. [Wang, Chia-Yih] Natl Ctr Hlth Stat, Div Hlth & Nutr Examinat Surveys, Hyattsville, MD 20782 USA. [Loria, Catherine M.] NHLBI, Ctr Dis Control & Prevent, NIH, Bethesda, MD 20892 USA. [Moshfegh, Alanna J.; Rhodes, Donna G.] USDA ARS, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Carriquiry, Alicia L.] Iowa State Univ, Dept Stat, Ames, IA USA. RP Mercado, CI (reprint author), Ctr Dis Control & Prevent, Mailstop F-72,4770 Buford Highway NE, Atlanta, GA 30341 USA. EM cmercado@cdc.gov FU CDC FX Data collection and laboratory analyses were funded by the CDC. NR 52 TC 8 Z9 8 U1 7 U2 11 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 2015 VL 101 IS 2 BP 376 EP 386 DI 10.3945/ajcn.113.081604 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA5US UT WOS:000348973900018 PM 25646336 ER PT J AU Smith, AD de Jager, CA Refsum, H Rosenberg, IH AF Smith, A. David de Jager, Celeste A. Refsum, Helga Rosenberg, Irwin H. TI Homocysteine lowering, B vitamins, and cognitive aging SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Letter ID DECLINE; DISEASE; TRIALS C1 [Smith, A. David] Univ Dept Pharmacol, OPTIMA, Oxford, England. [de Jager, Celeste A.] Univ Cape Town, Dept Med, ZA-7925 Cape Town, South Africa. [Refsum, Helga] Univ Oslo, Dept Nutr, Inst Basic Med Sci, Oslo, Norway. [Rosenberg, Irwin H.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Medford, MA USA. RP Smith, AD (reprint author), Univ Dept Pharmacol, OPTIMA, Oxford, England. EM david.smith@pharm.ox.ac.uk NR 10 TC 6 Z9 6 U1 0 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD FEB PY 2015 VL 101 IS 2 BP 415 EP 416 DI 10.3945/ajcn.114.098467 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA5US UT WOS:000348973900025 PM 25646343 ER PT J AU Egger-Danner, C Cole, JB Pryce, JE Gengler, N Heringstad, B Bradley, A Stock, KF AF Egger-Danner, C. Cole, J. B. Pryce, J. E. Gengler, N. Heringstad, B. Bradley, A. Stock, K. F. TI Invited review: overview of new traits and phenotyping strategies in dairy cattle with a focus on functional traits SO ANIMAL LA English DT Review DE phenotypes; novel traits; dairy cows; functional traits; genomics ID SOMATIC-CELL COUNT; NORWEGIAN RED COWS; MILK COAGULATION PROPERTIES; TECHNICAL-NOTE ADJUSTMENT; BODY CONDITION SCORE; DIRECT HEALTH TRAITS; GENETIC-PARAMETERS; GENOMIC SELECTION; CLINICAL MASTITIS; SHORT-COMMUNICATION AB For several decades, breeding goals in dairy cattle focussed on increased milk production. However, many functional traits have negative genetic correlations with milk yield, and reductions in genetic merit for health and fitness have been observed. Herd management has been challenged to compensate for these effects and to balance fertility, udder health and metabolic diseases against increased production to maximize profit without compromising welfare. Functional traits, such as direct information on cow health, have also become more important because of growing concern about animal well-being and consumer demands for healthy and natural products. There are major concerns about the impact of drugs used in veterinary medicine on the spread of antibiotic-resistant strains of bacteria that can negatively impact human health. Sustainability and efficiency are also increasingly important because of the growing competition for high-quality, plant-based sources of energy and protein. Disruptions to global environments because of climate change may encourage yet more emphasis on these traits. To be successful, it is vital that there be a balance between the effort required for data recording and subsequent benefits. The motivation of farmers and other stakeholders involved in documentation and recording is essential to ensure good data quality. To keep labour costs reasonable, existing data sources should be used as much as possible. Examples include the use of milk composition data to provide additional information about the metabolic status or energy balance of the animals. Recent advances in the use of mid-infrared spectroscopy to measure milk have shown considerable promise, and may provide cost-effective alternative phenotypes for difficult or expensive-to-measure traits, such as feed efficiency. There are other valuable data sources in countries that have compulsory documentation of veterinary treatments and drug use. Additional sources of data outside of the farm include, for example, slaughter houses (meat composition and quality) and veterinary labs (specific pathogens, viral loads). At the farm level, many data are available from automated and semi-automated milking and management systems. Electronic devices measuring physiological status or activity parameters can be used to predict events such as oestrus, and also behavioural traits. Challenges concerning the predictive biology of indicator traits or standardization need to be solved. To develop effective selection programmes for new traits, the development of large databases is necessary so that high-reliability breeding values can be estimated. For expensive-to-record traits, extensive phenotyping in combination with genotyping of females is a possibility. C1 [Egger-Danner, C.] ZuchtData EDV Dienstleistungen GmbH, A-1200 Vienna, Austria. [Cole, J. B.] USDA ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Pryce, J. E.] La Trobe Univ, Dept Environm & Primary Ind, Bundoora, Vic 3083, Australia. [Gengler, N.] Univ Liege, Gembloux Agrobio Tech GxABT, Anim Sci Unit, B-5030 Gembloux, Belgium. [Heringstad, B.] Norwegian Univ Life Sci, Dept Anim & Aquacultural Sci, N-1432 As, Norway. [Bradley, A.] Qual Milk Management Serv Ltd, Wells BA5 1EY, Somerset, England. [Bradley, A.] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics, England. [Stock, K. F.] Vereinigte Informat Syst Tierhaltung WV Vit, D-27283 Verden, Germany. RP Egger-Danner, C (reprint author), ZuchtData EDV Dienstleistungen GmbH, Dresdner Str 89-19, A-1200 Vienna, Austria. EM egger-danner@zuchtdata.at RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Federal Ministry of Agriculture, Forestry, Environment and Water Management (BMLFUW) in Austria; Federation of Austrian Cattle Breeders; 'Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information', of the Agricultural Research Service of the United States Department of Agriculture [1265-31000-096-00]; INTERREG IVB NWE Project OptiMIR: new tools for a more sustainable dairy sector [190G]; Ministry of Agriculture of the Walloon Region of Belgium (Service Public de Wallonie, Direction generale operationnelle 'Agriculture, Ressources naturelles et Environnement' - DGARNE) [D31-1248, D31-1304]; Department of Environment and Primary Industries (Melbourne, Australia) FX This review is based on an invited presentation at the 64th Annual Meeting of the European Association for Animal Production held in Nantes, France, August 2013. Excellent cooperation within ICAR Working Group Functional Traits. Federal Ministry of Agriculture, Forestry, Environment and Water Management (BMLFUW) in Austria and the Federation of Austrian Cattle Breeders for the support within the projects 'Efficient cow' and 'OptiGene'. Gene2Farm (EU-FP7-KBBE-2011-5-PNr.: 289592) for provision of information. J. B. Cole was supported by appropriated project 1265-31000-096-00, 'Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information', of the Agricultural Research Service of the United States Department of Agriculture. N. Gengler was supported by the INTERREG IVB NWE Project 190G OptiMIR: new tools for a more sustainable dairy sector and the Ministry of Agriculture of the Walloon Region of Belgium (Service Public de Wallonie, Direction generale operationnelle 'Agriculture, Ressources naturelles et Environnement' - DGARNE) for the support within the project 'MethaMilk' (D31-1248 and D31-1304). J. E. Pryce acknowledges financial support from the Department of Environment and Primary Industries (Melbourne, Australia). NR 169 TC 24 Z9 24 U1 13 U2 93 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD FEB PY 2015 VL 9 IS 2 BP 191 EP 207 DI 10.1017/S1751731114002614 PG 17 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AZ8XM UT WOS:000348495100001 PM 25387784 ER PT J AU Lee, KW Kim, DK Lillehoj, HS Jang, SI Lee, SH AF Lee, Kyung-Woo Kim, Duk Kyung Lillehoj, Hyun S. Jang, Seung I. Lee, Sung-Hyen TI Immune modulation by Bacillus subtilis-based direct-fed microbials in commercial broiler chickens SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Direct-fed microbials; Immune modulation; Chickens ID NECROTIC ENTERITIS; GENE-EXPRESSION; NITRIC-OXIDE; CLOSTRIDIUM-PERFRINGENS; GANGRENOUS DERMATITIS; GROWTH-PERFORMANCE; CYTOKINE RESPONSES; EIMERIA-MAXIMA; IN-VITRO; COCCIDIOSIS AB Direct-fed microbials (DFMs), also known as probiotics, have been successfully used to improve the balance of gut microbiota. Spores of Bacillus subtilis, have been used as DFMs for food animals and humans and our previous studies showed that dietary supplementation of broiler chickens with a Bacillus subtilis DFM from hatch enhanced innate immunity and improved protection against avian coccidiosis. In two experiments, we investigated the effects of dietary Bacillus subtilis-based DFM on growth performance and host humoral immune response to Eimeria spp. and Clostridium perfringens, and evaluated Bacillus subtills-induced global gene expression changes in broiler chickens. Day-old broiler chickens were fed diets with or without DFMs for 28 days starting from hatch. At 14 and 28 days post-hatch, broiler chickens fed the DFM-enriched diet gained significantly more weight (P<0.05) compared with the control diet-fed chickens. Broiler chickens fed diet containing DFM exhibited a significant decrease (P=0.033) in Eimeria- and Clostridium perfringens necrotic enteritis B-like (NetB) toxin-specific serum antibodies compared with those fed a control diet at day 28. The levels of transcripts encoding interleukin (IL) 1 beta, IL12 and interferon-gamma were greater (P=0.004) in the DFM-fed chickens compared with the control chickens. In order to better understand Bacillus subtilis-induced molecular changes associated with improved innate immunity, mRNA was isolated from mid-intestine of DFM-fed chickens for global microarray analysis. Dietary DFMs induced considerable changes in transcriptional expression in intestine, and biofunctional analysis identified 37 genes related with "Inflammatory Response". These results indicate that dietary Bacillus subtilis DFM is an effective growth-promoting dietary supplement to increase growth and augment innate immune response in broiler chickens. Further study is needed to investigate the mechanism mediated by dietary DFMs to enhance protective innate immunity in a disease model (i.e., necrotic enteritis) which causes considerable economic impact in the poultry industry worldwide. Published by Elsevier B.V. C1 [Lee, Kyung-Woo; Kim, Duk Kyung; Lillehoj, Hyun S.; Jang, Seung I.; Lee, Sung-Hyen] USDA ARS, Beltsville Agr Res Ctr, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Lee, Kyung-Woo] Konkuk Univ, Dept Anim Sci & Technol, Seoul 143701, South Korea. [Kim, Duk Kyung] Hankyong Natl Univ, Anim Genom & Breeding Ctr, Ansong 456749, South Korea. [Lee, Sung-Hyen] Natl Acad Agr Sci, Rural Dev Adm, Jeollabuk Do 565851, South Korea. RP Lillehoj, HS (reprint author), USDA ARS, Beltsville Agr Res Ctr, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ARS.USDA.GOV OI Lee, Kyung-Woo/0000-0002-3533-7979 FU ARS in-house project [1245-32000-097]; Next-Generation BioGreen 21 Program [PJ008196]; project "Investigation of the functional activity and the development of functional foods of Allium hookeri" of Rural Development Administration, Korea [PJ010490]; ARS-USDA [1265-32000-086-15T]; Danisco [1265-32000-086-15T] FX This project was supported, in part, by the ARS in-house project (#1245-32000-097), the Next-Generation BioGreen 21 Program (No. PJ008196) and project "Investigation of the functional activity and the development of functional foods of Allium hookeri (PJ010490)" of Rural Development Administration, Korea, and partially by a Trust agreement (1265-32000-086-15T) established between ARS-USDA and Danisco. The authors thank Marjorie Nichols and Stacy Torreyson in Animal Biosciences and Biotechnology Laboratory of USDA-ARS for their technical assistance. We also thank Dr. Daniel A. Bautista in Lasher Poultry Diagnostic Laboratory, University of Delaware, and Dr. Gregory R. Siragusa in Danisco for providing us the experimental facility and the experimental diets for this study. NR 43 TC 3 Z9 4 U1 5 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 EI 1873-2216 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD FEB PY 2015 VL 200 BP 76 EP 85 DI 10.1016/j.anifeedsci.2014.12.006 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA CA9VC UT WOS:000349271900009 ER PT J AU Ragen, DL Nix, EE Endecott, RL Hatfield, PG Petersen, MK Bowman, JGP AF Ragen, Devon L. Nix, Erin E. Endecott, Rachel L. Hatfield, Patrick G. Petersen, Mark K. Bowman, Janice G. P. TI Individual mineral supplement intake by ewes swath grazing or confinement fed pea-barley forage SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Sheep; Mineral supplement; Swath grazing; Hay; Individual intake ID SHEEP; MOLASSES; DIGESTIBILITY; BLOCKS; CATTLE; UREA AB Previous research has reported high variation in intake of self-fed protein and/or energy supplements by individual animals, however little is known about variation in consumption of mineral supplements. Sixty mature range ewes (non-pregnant, non-lactating) were used in a completely randomized design repeated 2 years to determine if feeding method of intercropped field pea (Pisum sativum L.) and spring barley (Hordeum vulgare L.) forage (swath grazed or fed as hay in confinement) affected individual ewe mineral consumption. Thirty ewes were allocated to 3 confinement pens (10 ewes/pen) and 30 ewes were allocated to 3 grazing plots (10 ewes/plot). Ewes had ad libitum access to feed, water, and a mineral supplement containing 1% titanium dioxide as an external marker. Forage dry matter intake (DMI) was calculated using estimates of fecal output, and in vitro 48-h for-age DM digestibility. Ewe supplement intake was determined from fecal and supplement Ti concentrations, and fecal output. Forage and mineral intakes were analyzed using ewe as the experimental unit, and plot or pen as the experimental unit for intake variation. A year x treatment interaction (P<0.01) existed for DM forage and mineral intake. Ewes in confinement consumed more forage DM than grazing ewes in 2010, but less than grazing ewes in 2011. Mean mineral intake was highest (P<0.01) by grazing ewes in 2011 and 2010 (average 69 g/day), intermediate by confinement ewes in 2010 (57 g/day), and lowest by confinement ewes in 2011 (31 g/day). A yearxtreatment interaction (P=0.05) existed for mineral intake CV which was higher (P=0.04) for confinement ewes in 2011 (67 vs. 34%), but was not different (P>0.05) between treatments in 2010. In this study, variation in individual ewe intake of mineral supplement was large in both grazing ewes and ewes fed hay in confinement. Published by Elsevier B.V. C1 [Ragen, Devon L.; Nix, Erin E.; Endecott, Rachel L.; Hatfield, Patrick G.; Bowman, Janice G. P.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59718 USA. [Petersen, Mark K.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Bowman, JGP (reprint author), Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59718 USA. EM jbowman@montana.edu FU Bair Ranch Foundation, Billings, MT FX This work was supported in part by the Bair Ranch Foundation, Billings, MT. NR 17 TC 0 Z9 0 U1 2 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 EI 1873-2216 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD FEB PY 2015 VL 200 BP 107 EP 111 DI 10.1016/j.anifeedsci.2014.12.003 PG 5 WC Agriculture, Dairy & Animal Science SC Agriculture GA CA9VC UT WOS:000349271900013 ER PT J AU Casas, E Hessman, BE Keele, JW Ridpath, JF AF Casas, E. Hessman, B. E. Keele, J. W. Ridpath, J. F. TI A genome-wide association study for the incidence of persistent bovine viral diarrhea virus infection in cattle SO ANIMAL GENETICS LA English DT Article DE BVDV; diseases; genome scan; persistent infection ID CHRONIC KIDNEY-DISEASE; RENAL-FUNCTION; FAMILY; LOCI; POPULATION; CERNUNNOS; MUTATION; REGIONS; FEEDLOT; TRAITS AB Bovine viral diarrhea viruses (BVDV) comprise a diverse group of viruses that cause disease in cattle. BVDV may establish both transient and persistent infections depending on the developmental stage of the animal at exposure. The objective was to determine whether genomic regions harboring single nucleotide polymorphisms (SNPs) could be associated with the presence or absence of persistent BVDV infection. A genome-wide association approach based on 777000 SNP markers was used. Samples of animals identified as positive (n=1200) or negative (n=1200) for the presence of BVDV in skin samples (n=1200) were used. DNA samples were combined in 24 pools (100 animals per pool). One SNP, significant at the 5 percent genome-wide level (P=9.41x10(-8)), was detected on chromosome 14, located at position 80675176bp. Fifteen SNPs, residing on chromosomes 1, 2, 6, 8, 10, 15 and 18, were moderately associated (P<1x10(-5)) with persistent BVDV infection. Results show that genes harboring or neighboring significant SNPs are involved in leucopenia, signal transduction, RNA splicing and DNA methylation processes. C1 [Casas, E.; Ridpath, J. F.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Hessman, B. E.] Haskell Cty Anim Hosp, Sublette, KS USA. [Keele, J. W.] ARS, US Meat Anim Res Ctr, USDA, Clay Ctr, NE USA. RP Casas, E (reprint author), ARS, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM Eduardo.Casas@ARS.USDA.GOV NR 39 TC 4 Z9 4 U1 1 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2015 VL 46 IS 1 BP 8 EP 15 DI 10.1111/age.12239 PG 8 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CA1MZ UT WOS:000348677900002 PM 25394207 ER PT J AU Lindholm-Perry, AK Kuehn, LA Freetly, HC Snelling, WM AF Lindholm-Perry, A. K. Kuehn, L. A. Freetly, H. C. Snelling, W. M. TI Genetic markers that influence feed efficiency phenotypes also affect cattle temperament as measured by flight speed SO ANIMAL GENETICS LA English DT Article DE average daily gain; beef steers; temperament; GLRB; GRIA2; QKI ID BEEF-CATTLE; TRAITS AB Flight speed is a predictive indicator of cattle temperament and is associated with feed efficiency phenotypes. Genetic markers associated with both traits may assist with selection of calmer animals with improved economic value. A preliminary genome-wide association study determined chromosomal regions on BTA9, and 17 were associated with flight speed. The genes quaking (QKI), glutamate receptor, ionotropic, AMPA 2 (GRIA2) and glycine receptor (GLRB) were identified in these regions as potential functional candidates. Beef steers (n=1057) were genotyped with SNPs located within and flanking these genes. One SNP located near QKI and one near GRIA2 were nominally associated with flight speed (P0.05) although neither was significant after Bonferroni correction. Several studies have shown a correlation between flight speed and feed intake or gain; therefore, we also analyzed SNPs on BTA6:38-39Mb known to be associated with average daily gain (ADG) and average daily feed intake (ADFI) for association with flight speed. Several SNPs on BTA6 were associated with flight speed (P0.005), and three were significant after Bonferroni correction. These results suggest that the genes tested are unlikely to contribute to flight speed variation for our cattle population, but SNPs on BTA6 associated with ADG and ADFI may influence temperament. Use of these markers to select for economically important feed efficiency phenotypes may produce cattle with more desirable temperaments. C1 [Lindholm-Perry, A. K.; Kuehn, L. A.; Freetly, H. C.; Snelling, W. M.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Lindholm-Perry, AK (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM Amanda.Lindholm@ars.usda.gov NR 11 TC 4 Z9 4 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD FEB PY 2015 VL 46 IS 1 BP 60 EP 64 DI 10.1111/age.12244 PG 5 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA CA1MZ UT WOS:000348677900009 PM 25515066 ER PT J AU Sawhney, N Crooks, C John, FS Preston, JF AF Sawhney, Neha Crooks, Casey John, Franz St. Preston, James F. TI Transcriptomic Analysis of Xylan Utilization Systems in Paenibacillus sp Strain JDR-2 SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID BACTERIUM THERMOTOGA-MARITIMA; UTILIZATION GENE-CLUSTER; BACILLUS-SUBTILIS; ETHANOL-PRODUCTION; GEOBACILLUS-STEAROTHERMOPHILUS; LIGNOCELLULOSIC BIOMASS; ALDOURONATE UTILIZATION; THERMOPHILIC BACTERIA; ALPHA-GLUCURONIDASE; ESCHERICHIA-COLI AB Xylans, including methylglucuronoxylans (MeGX(n)) and methylglucuronoarabinoxylans (MeGAX(n)), are the predominant polysaccharides in hemicellulose fractions of dicots and monocots available for conversion to biofuels and chemicals. Paenibacillus sp. strain JDR-2 (Pjdr2) efficiently depolymerizes MeGX(n) and MeGAX(n) and assimilates the generated oligosaccharides, resulting in efficient saccharification and subsequent metabolism of these polysaccharides. A xylan utilization regulon encoding a cell-associated GH10 (glycoside hydrolase family 10) endoxylanase, transcriptional regulators, ABC (ATP binding cassette) transporters, an intracellular GH67 alpha-glucuronidase, and other glycoside hydrolases contributes to complete metabolism. This GH10/GH67 system has been proposed to account for preferential utilization of xylans compared to free oligo-and monosaccharides. To identify additional genes contributing to MeGX(n) and MeGAX(n) utilization, the transcriptome of Pjdr2 has been sequenced following growth on each of these substrates as well as xylose and arabinose. Increased expression of genes with different substrates identified pathways common or unique to the utilization of MeGX(n) or MeGAX(n). Coordinate upregulation of genes comprising the GH10/GH67 xylan utilization regulon is accompanied with upregulation of genes encoding a GH11 endoxylanase and a GH115 alpha-glucuronidase, providing evidence for a novel complementary pathway for processing xylans. Elevated expression of genes encoding a GH43 arabinoxylan arabinofuranohydrolase and an arabinose ABC transporter on MeGAX(n) but not on MeGX(n) supports a process in which arabinose may be removed extracellularly followed by its rapid assimilation. Further development of Pjdr2 for direct conversion of xylans to targeted products or introduction of these systems into fermentative strains of related bacteria may lead to biocatalysts for consolidated bio processing of hemicelluloses released from lignocellulose. C1 [Sawhney, Neha; Preston, James F.] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. [Crooks, Casey; John, Franz St.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. RP Preston, JF (reprint author), Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA. EM jpreston@ufl.edu RI St John, Franz/J-8970-2016 OI St John, Franz/0000-0003-3458-5628 FU USDA National Institute of Food and Agriculture [2011-10006-30358]; Florida Energy Systems Consortium, State University System of Florida [00077818]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX This research was supported by Biomass Research & Development Initiative competitive grant 2011-10006-30358 from the USDA National Institute of Food and Agriculture and by Florida Energy Systems Consortium, State University System of Florida, project 00077818. The RNA sequencing work at the Joint Genome Institute, Walnut Creek, CA, was supported by the Office of Science of the U.S. Department of Energy under contract DE-AC02-05CH11231. NR 48 TC 6 Z9 6 U1 7 U2 25 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2015 VL 81 IS 4 BP 1490 EP 1501 DI 10.1128/AEM.03523-14 PG 12 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA5DF UT WOS:000348927600030 PM 25527555 ER PT J AU Polivka, KM Steel, EA Novak, JL AF Polivka, Karl M. Steel, E. Ashley Novak, Jenni L. TI Juvenile salmon and steelhead occupancy of stream pools treated and not treated with restoration structures, Entiat River, Washington SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID HABITAT SELECTION; CHINOOK SALMON; ONCORHYNCHUS-MYKISS; FISH-HABITAT; COHO SALMON; CALIFORNIA STREAM; CUTTHROAT TROUT; LARGE WOOD; ABUNDANCE; COMPETITION AB We observed habitat occupancy by juvenile Chinook salmon (Oncorhynchus tschawytscha) and steelhead trout (Oncorhynchus mykiss) at in-stream habitat restoration structures constructed in the Entiat River, Washington, USA. In 2009-2013, fish abundance measurements during rearing (July-October) showed high temporal variability in pools with restoration structures. Both species were more abundant at restored pools than at natural pools in early summer (July), but this difference was typically absent by September. Fish response to restoration structures also varied across years. When looking only at restored pools, there were strong seasonal fluxes in parameters describing the effects of temperature, water depth, and current velocity on fish abundance. Significant interaction terms such as current velocity x depth and temperature x current velocity were present for both species, suggesting that these may be important physical attributes improved by restoration. Through extensive sampling in untreated habitat, both within the treated segment and in nearby control segments, we found that when higher Chinook abundance was observed at restored pools, it was apparently attributable to an increase in habitat capacity and not due to depletion of fish from natural habitat in the same segment. Steelhead habitat selection was too inconsistent for conclusions about capacity, but we did not observe evidence that structures depleted untreated habitat. C1 [Polivka, Karl M.; Steel, E. Ashley] USDA Forest Serv, Pacific NW Res Stn, Land & Watershed Management Program, Wenatchee, WA 98801 USA. [Novak, Jenni L.] Cascadia Conservat Dist, Wenatchee, WA USA. RP Polivka, KM (reprint author), USDA Forest Serv, Pacific NW Res Stn, Land & Watershed Management Program, 1133 N Western Ave, Wenatchee, WA 98801 USA. EM kpolivka@fs.fed.us FU Bonneville Power Administration [2003-017-00]; American Recovery and Re-investment Act; American Fisheries Society Hutton Junior Fisheries Biology Scholarship FX This work was supported by Bonneville Power Administration (No. 2003-017-00), administered through the Integrated Status and Effectiveness Monitoring Program (ISEMP), managed by C. Jordan (NOAA-Fisheries) and P. Nelle (Terraqua, Inc.). Additional support was provided by the American Recovery and Re-investment Act enacted by President B. Obama. We thank S. Eichler, R. Logan, J. Jorgensen, H. Porter, L. Flynn, K. Sirianni, K. Tackman, A. Bushy, J. West, and K. Logan for assistance in data collection and preparation. A. Bushy was supported by the American Fisheries Society Hutton Junior Fisheries Biology Scholarship. Reviews of earlier drafts were provided by S. Hays, T. Hillman, C. Peven, G. Maier (Upper Columbia Regional Technical Team), and A. Rosenberger. NR 53 TC 0 Z9 0 U1 6 U2 17 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD FEB PY 2015 VL 72 IS 2 BP 166 EP 174 DI 10.1139/cjfas-2014-0228 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CA3GX UT WOS:000348795800002 ER PT J AU Falke, JA Flitcroft, RL Dunham, JB McNyset, KM Hessburg, PF Reeves, GH AF Falke, Jeffrey A. Flitcroft, Rebecca L. Dunham, Jason B. McNyset, Kristina M. Hessburg, Paul F. Reeves, Gordon H. TI Climate change and vulnerability of bull trout (Salvelinus confluentus) in a fire-prone landscape SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID COLUMBIA RIVER-BASIN; WESTERN UNITED-STATES; PACIFIC-NORTHWEST; CUTTHROAT TROUT; RAINBOW-TROUT; NATIVE FISHES; STREAM TEMPERATURES; WASHINGTON-STATE; THERMAL REGIMES; WILDFIRE AB Linked atmospheric and wildfire changes will complicate future management of native coldwater fishes in fire-prone landscapes, and new approaches to management that incorporate uncertainty are needed to address this challenge. We used a Bayesian network (BN) approach to evaluate population vulnerability of bull trout (Salvelinus confluentus) in the Wenatchee River basin, Washington, USA, under current and future climate and fire scenarios. The BN was based on modeled estimates of wildfire, water temperature, and physical habitat prior to, and following, simulated fires throughout the basin. We found that bull trout population vulnerability depended on the extent to which climate effects can be at least partially offset by managing factors such as habitat connectivity and fire size. Moreover, our analysis showed that local management can significantly reduce the vulnerability of bull trout to climate change given appropriate management actions. Tools such as our BN that explicitly integrate the linked nature of climate and wildfire, and incorporate uncertainty in both input data and vulnerability estimates, will be vital in effective future management to conserve native coldwater fishes. C1 [Falke, Jeffrey A.; McNyset, Kristina M.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97333 USA. [Flitcroft, Rebecca L.; Reeves, Gordon H.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97333 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97333 USA. [Hessburg, Paul F.] US Forest Serv, Pacific NW Res Stn, USDA, Wenatchee, WA 98801 USA. RP Falke, JA (reprint author), Univ Alaska Fairbanks, US Geol Survey, Alaska Cooperat Fish & Wildlife Res Unit, POB 757020, Fairbanks, AK 99775 USA. EM jeffrey.falke@alaska.edu FU Joint Fire Science Program; American Recovery and Reinvestment Act through the US Forest Service Pacific Northwest Research Station; DOI Northwest Climate Science Center; National Research Council; Oregon State University FX This project was partially funded by a grant from the Joint Fire Science Program to G.H.R. and R.L.F., the American Recovery and Reinvestment Act through the US Forest Service Pacific Northwest Research Station, and the DOI Northwest Climate Science Center. Additional support for J.A.F. and K.M.M. was provided by the National Research Council and Oregon State University. The authors thank L. Benda, P. Bisson, W. Elliot, and S. Wondzell for advice on an early version of the project and K. Pearse for editorial assistance. A. Brookes, C. Jordan, K. Vance-Borland, and M. Weber assisted with study design and analysis. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US government. NR 97 TC 1 Z9 1 U1 8 U2 42 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD FEB PY 2015 VL 72 IS 2 BP 304 EP 318 DI 10.1139/cjfas-2014-0098 PG 15 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA CA3GX UT WOS:000348795800014 ER PT J AU Churchill, AC Turetsky, MR McGuire, AD Hollingsworth, TN AF Churchill, Amber C. Turetsky, Merritt R. McGuire, A. David Hollingsworth, Teresa N. TI Response of plant community structure and primary productivity to experimental drought and flooding in an Alaskan fen SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE primary productivity; peatland; hydrologic manipulation; drying; flooding ID WATER-TABLE MANIPULATIONS; CARBON-CYCLE; CO2 EXCHANGE; NORTHERN PEATLANDS; BOREAL PEATLANDS; LEVEL VARIATION; VEGETATION; DYNAMICS; BIOMASS; SEQUESTRATION AB Northern peatlands represent a long-term net sink for atmospheric CO2, but these ecosystems can shift from net carbon (C) sinks to sources based on changing climate and environmental conditions. In particular, changes in water availability associated with climate control peatland vegetation and carbon uptake processes. We examined the influence of changing hydrology on plant species abundance and ecosystem primary production in an Alaskan fen by manipulating the water table in field treatments to mimic either sustained flooding (raised water table) or drought (lowered water table) conditions for 6 years. We found that water table treatments altered plant species abundance by increasing sedge and grass cover in the raised water table treatment and reducing moss cover while increasing vascular green area in the lowered water table treatment. Gross primary productivity was lower in the lowered treatment than in the other plots, although there were no differences in total biomass or vascular net primary productivity among the treatments. Overall, our results indicate that vegetation abundance was more sensitive to variation in water table than total biomass and vascular biomass accrual. Finally, in our experimental peatland, drought had stronger consequences for change in vegetation abundance and ecosystem function than sustained flooding. C1 [Churchill, Amber C.] Univ Alaska Fairbanks, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. [Turetsky, Merritt R.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [McGuire, A. David] Univ Alaska, US Geol Survey, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Hollingsworth, Teresa N.] Univ Alaska Fairbanks, US Forest Serv, Pacific NW Res Stn, Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. RP Churchill, AC (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80303 USA. EM Amber.Churchill@colorado.edu FU National Science Foundation (NSF) [DEB-0425328, DEB-0724514, DEB-0830997]; Bonanza Creek Long-Term Ecological Research program - NSF [DEB-0620579]; Bonanza Creek Long-Term Ecological Research program - USDA Forest Service Pacific Northwest Research [PNW01-JV11261952-231] FX Previous versions of this manuscript were improved by comments from the CU EBIO Writing Seminar, Kim Wickland, and Gauis Shaver. Jennifer Harden helped design the study and provided thoughtful comments on this manuscript. We thank Katie Shea, Nicole McConnell, Caitlin Lawrence, Bill Cable, Molly Chivers, Claire Treat, Grant Austin, Chris Dorich, Katie Neufeld, Sara Klapstein, Dana Nossov, Collin Maachel, Nick Brehm, Korlyn Bolster, Matthew Smith, Mike Waddington, and Mark Waldrop for field and laboratory assistance. We are grateful to the Bonanza Creek LTER for laboratory space and logistics. This research was supported by National Science Foundation (NSF) grants supporting the APEX project (DEB-0425328, DEB-0724514, and DEB-0830997) and by the Bonanza Creek Long-Term Ecological Research program (funded jointly by NSF grant DEB-0620579 and USDA Forest Service Pacific Northwest Research grant PNW01-JV11261952-231). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 49 TC 2 Z9 3 U1 10 U2 51 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD FEB PY 2015 VL 45 IS 2 BP 185 EP 193 DI 10.1139/cjfr-2014-0100 PG 9 WC Forestry SC Forestry GA AZ9IB UT WOS:000348526000006 ER PT J AU Rossdeutscher, L Li, J Luco, AL Fadhil, I Ochietti, B Camirand, A Huang, DC Reinhardt, TA Muller, W Kremer, R AF Rossdeutscher, Lionel Li, Jiarong Luco, Aimee-Lee Fadhil, Ibtihal Ochietti, Benoit Camirand, Anne Huang, Dao Chao Reinhardt, Timothy A. Muller, William Kremer, Richard TI Chemoprevention Activity of 25-Hydroxyvitamin D in the MMTV-PyMT Mouse Model of Breast Cancer SO CANCER PREVENTION RESEARCH LA English DT Article ID ESTROGEN-RECEPTOR-ALPHA; VITAMIN-D DEFICIENCY; MAMMARY-GLAND; PROSTATE-CANCER; UNITED-STATES; D METABOLISM; EXPRESSION; GROWTH; 1,25-DIHYDROXYVITAMIN-D; PREVENTION AB Development of oncologic conditions is often accompanied by inadequate vitamin D status. The chemoprevention ability of this molecule is of high interest for breast cancer, the most common malignancy in women worldwide. Because current effective vitamin D analogues, including the naturally occurring active metabolite 1,25-dihydroxycholecalciferol (1,25(OH) 2D), frequently cause hypercalcemia at pharmacologic doses, the development of safer molecules for clinical chemopreventive use is essential. This study examines whether exogenously supplied prohormone 25hydroxycholecalciferol (25(OH) D) can delay tumor progression in vivo without hypercalcemic effects. A lowvitaminDdiet (25 IU/kg) in the non-immunodeficient MMTV-PyMT mouse model of metastatic breast cancer revealed a significant acceleration ofmammary neoplasia compared with normal diet (1,000 IU/kg). Systemic perfusion of MMTV-PyMT mice with 25(OH) D or 1,25(OH) 2D delayed tumor appearance and significantly decreased lungmetastasis, and both metabolites reduced Ki-67, cyclin D1, and ErbB2 levels in tumors. Perfusion with 25(OH) D caused a 50% raise in tumor 1,25(OH) 2D levels, indicating good tumor penetration and effective activation. Importantly, in contrast with 1,25(OH) 2D, perfusion with 25(OH) D did not cause hypercalcemia. In vitro treatment of culturedMMTV-PyMT mammary tumor cells with 25 (OH) D inhibited proliferation, confirming local activation of the prohormone in this system. This study provides an in vivo demonstration in a non-immunodeficientmodel of spontaneous breast cancer that exogenous 25(OH) D delays neoplasia, tumor growth, and metastasis, and that its chemoprevention efficacy is not accompanied by hypercalcemia. (C) 2014 AACR. C1 [Rossdeutscher, Lionel; Li, Jiarong; Luco, Aimee-Lee; Fadhil, Ibtihal; Ochietti, Benoit; Camirand, Anne; Huang, Dao Chao; Kremer, Richard] McGill Univ, Ctr Hlth, Dept Med, Montreal, PQ, Canada. [Reinhardt, Timothy A.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Muller, William] McGill Univ, Goodman Canc Res Ctr, Montreal, PQ, Canada. RP Kremer, R (reprint author), Royal Victoria Hosp, Calcium Res Lab, Dept Med, Room H4-67,687 Ouest Ave Pins, Montreal, PQ H3A 1A1, Canada. EM richard.kremer@mcgill.ca FU Canadian Institutes for Health Research (CIHR) [MOP 10839] FX This work was supported by Canadian Institutes for Health Research (CIHR) grant MOP 10839 to R. Kremer. NR 66 TC 6 Z9 6 U1 0 U2 7 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1940-6207 EI 1940-6215 J9 CANCER PREV RES JI Cancer Prev. Res. PD FEB PY 2015 VL 8 IS 2 BP 120 EP 128 DI 10.1158/1940-6207.CAPR-14-0110 PG 9 WC Oncology SC Oncology GA CA4WM UT WOS:000348907800004 PM 25468832 ER PT J AU Cao, Y Zavaterri, P Youngblood, J Moon, R Weiss, J AF Cao, Yizheng Zavaterri, Pablo Youngblood, Jeff Moon, Robert Weiss, Jason TI The influence of cellulose nanocrystal additions on the performance of cement paste SO CEMENT & CONCRETE COMPOSITES LA English DT Article DE Cellulose nanocrystal; CNC; Degree of hydration; Ball-on-three-ball flexural test; Steric stabilization; Short-circuit diffusion ID 3 BALLS TEST; BRITTLE DISCS; ZETA; MICROSTRUCTURE; COMPOSITES; BEHAVIOR AB The influence of cellulose nanocrystals (CNCs) addition on the performance of cement paste was investigated. Our mechanical tests show an increase in the flexural strength of approximately 30% with only 0.2% volume of CNCs with respect to cement. Isothermal calorimetry (IC) and thermogravimetric analysis (TGA) show that the degree of hydration (DOH) of the cement paste is increased when CNCs are used. The first mechanism that may explain the increased hydration is the steric stabilization, which is the same mechanism by which many water reducing agents (WRAs) disperse the cement particles. Rheological, heat flow rate measurements, and microscopic imaging support this mechanism. A second mechanism also appears to support the increased hydration. The second mechanism that is proposed is referred to as short circuit diffusion. Short circuit diffusion appears to increase cement hydration by increasing the transport of water from outside the hydration product shell (i.e., through the high density CSH) on a cement grain to the unhydrated cement cores. The DOH and flexural strength were measured for cement paste with WRA and CNC to evaluate this hypothesis. Our results indicate that short circuit diffusion is more dominant than steric stabilization. (C) 2014 Published by Elsevier Ltd. C1 [Cao, Yizheng; Youngblood, Jeff; Weiss, Jason] Purdue Univ, Sch Mat Sci & Engn, W Lafayette, IN 47907 USA. [Zavaterri, Pablo; Weiss, Jason] Purdue Univ, Sch Civil Engn, W Lafayette, IN 47907 USA. [Moon, Robert] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Weiss, J (reprint author), Purdue Univ, Sch Civil Engn, W Lafayette, IN 47907 USA. OI Weiss, William/0000-0003-2859-7980 FU National Science Foundation [1131596] FX This research was supported by the National Science Foundation through Awards no. CMMI #1131596. The authors are grateful to Carlos Martinez for supplying the rheometer and for many stimulating discussions on the rheological properties of CNC suspensions. We are grateful for Rick Reiner from the USFS Forest Products Laboratory for providing the CNC suspensions. NR 30 TC 10 Z9 10 U1 3 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0958-9465 EI 1873-393X J9 CEMENT CONCRETE COMP JI Cem. Concr. Compos. PD FEB PY 2015 VL 56 BP 73 EP 83 DI 10.1016/j.cemconcomp.2014.11.008 PG 11 WC Construction & Building Technology; Materials Science, Composites SC Construction & Building Technology; Materials Science GA CA0SZ UT WOS:000348628000008 ER PT J AU Oluyomi, AO Byars, A Byrd-Williams, C Sharma, SV Durand, C Hoelscher, DM Butte, NF Kelder, SH AF Oluyomi, Abiodun O. Byars, Allison Byrd-Williams, Courtney Sharma, Shreela V. Durand, Casey Hoelscher, Deanna M. Butte, Nancy F. Kelder, Steven H. TI The Utility of Geographical Information Systems (GIS) in Systems-Oriented Obesity Intervention Projects: The Selection of Comparable Study Sites for a Quasi-Experimental Intervention Design-TX CORD SO CHILDHOOD OBESITY LA English DT Article ID LOCAL FOOD ENVIRONMENT; BMI Z-SCORE; PHYSICAL-ACTIVITY; BUILT ENVIRONMENT; DIET QUALITY; WEIGHT-GAIN; ASSOCIATIONS; CHILDREN; HEALTH; ADOLESCENTS AB Background: The Texas Childhood Obesity Research Demonstration project (TX CORD) uses a systems-oriented approach to address obesity that includes individual and family interventions, community-level action, as well as environmental and policy initiatives. Given that randomization is seldom possible in community-level intervention studies, TX CORD uses a quasi-experimental design. Comparable intervention and comparison study sites are needed to address internal validity bias. Methods: TX CORD was designed to be implemented in low-income, ethnically diverse communities in Austin and Houston, Texas. A three-stage Geographical Information System (GIS) methodology was used to establish and ascertain the comparability of the intervention and comparison study sites. Census tract (stage 1) and school (stage 2) data were used to identify spatially exclusive geographic areas that were comparable. In stage 3, study sites were compared on demographic characteristics, socioeconomic status (SES), food assets, and physical activity (PA) assets. Student's t-test was used to examine significant differences between the selected sites. Results: The methodology that was used resulted in the selection of catchment areas with demographic and socioeconomic characteristics that fit the target population: ethnically diverse population; lower-median household income; and lower home ownership rates. Additionally, the intervention and comparison sites were statistically comparable on demographic and SES variables, as well as food assets and PA assets. Conclusions: This GIS approach can provide researchers, program evaluators, and policy makers with useful tools for both research and practice. Area-level information that allows for robust understanding of communities can enhance analytical procedures in community health research and offer significant contributions in terms of community assessment and engagement. C1 [Oluyomi, Abiodun O.; Byars, Allison; Byrd-Williams, Courtney; Sharma, Shreela V.; Durand, Casey; Hoelscher, Deanna M.; Kelder, Steven H.] Univ Texas Sch Publ Hlth, Michael & Susan Dell Ctr Hlth Living, Austin, TX 78701 USA. [Butte, Nancy F.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Oluyomi, AO (reprint author), Univ Texas Sch Publ Hlth, Michael & Susan Dell Ctr Hlth Living, Austin Reg Campus 1616 Guadalupe,Suite 6-300, Austin, TX 78701 USA. EM Abiodun.O.Oluyomi@uth.tmc.edu FU CDC [RFA-DP-11-007]; USDA/ARS [58-6250-0-008] FX This research was supported by cooperative agreement RFA-DP-11-007 from the CDC. The content is solely the responsibility of the authors and does not necessarily represent the official views of the CDC. Additional support was provided by the Michael and Susan Dell Center for Healthy Living. This work is a publication of the USDA (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and had been funded, in part, with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. NR 36 TC 3 Z9 3 U1 5 U2 14 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD FEB 1 PY 2015 VL 11 IS 1 BP 58 EP 70 DI 10.1089/chi.2014.0054 PG 13 WC Pediatrics SC Pediatrics GA CB0LH UT WOS:000349317000007 PM 25587670 ER PT J AU Hoelscher, DM Butte, NF Barlow, S Vandewater, EA Sharma, SV Huang, T Finkelstein, E Pont, S Sacher, P Byrd-Williams, C Oluyomi, AO Durand, C Li, LL Kelder, SH AF Hoelscher, Deanna M. Butte, Nancy F. Barlow, Sarah Vandewater, Elizabeth A. Sharma, Shreela V. Huang, Terry Finkelstein, Eric Pont, Stephen Sacher, Paul Byrd-Williams, Courtney Oluyomi, Abiodun O. Durand, Casey Li, Linlin Kelder, Steven H. TI Incorporating Primary and Secondary Prevention Approaches To Address Childhood Obesity Prevention and Treatment in a Low-Income, Ethnically Diverse Population: Study Design and Demographic Data from the Texas Childhood Obesity Research Demonstration (TX CORD) Study SO CHILDHOOD OBESITY LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; QUALITY-OF-LIFE; PHYSICAL-ACTIVITY; MEND PROGRAM; PRIMARY-CARE; CHILDREN; HEALTH; NUTRITION; OVERWEIGHT; VALIDITY AB Background: There is consensus that development and evaluation of a systems-oriented approach for child obesity prevention and treatment that includes both primary and secondary prevention efforts is needed. This article describes the study design and baseline data from the Texas Childhood Obesity Research Demonstration (TX CORD) project, which addresses child obesity among low-income, ethnically diverse overweight and obese children, ages 2-12 years; a two-tiered systems-oriented approach is hypothesized to reduce BMI z-scores, compared to primary prevention alone. Methods: Our study aims are to: (1) implement and evaluate a primary obesity prevention program; (2) implement and evaluate efficacy of a 12-month family-centered secondary obesity prevention program embedded within primary prevention; and (3) quantify the incremental cost-effectiveness of the secondary prevention program. Baseline demographic and behavioral data for the primary prevention community areas are presented. Results: Baseline data from preschool centers, elementary schools, and clinics indicate that most demographic variables are similar between intervention and comparison communities. Most families are low income (<=$25,000) and Hispanic/Latino (73.3-83.8%). The majority of parents were born outside of the United States. Child obesity rates exceed national values, ranging from 19.0% in preschool to 35.2% in fifth-grade children. Most parents report that their children consume sugary beverages, have a television in the bedroom, and do not consume adequate amounts of fruits and vegetables. Conclusions: Interventions to address childhood obesity are warranted in low-income, ethnically diverse communities. Integrating primary and secondary approaches is anticipated to provide sufficient exposure that will lead to significant decreases in childhood obesity. C1 [Hoelscher, Deanna M.; Vandewater, Elizabeth A.; Byrd-Williams, Courtney; Oluyomi, Abiodun O.; Li, Linlin; Kelder, Steven H.] Univ Texas Sch Publ Hlth, Michael & Susan Dell Ctr Hlth Living, Austin, TX 78701 USA. [Butte, Nancy F.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Barlow, Sarah] Texas Childrens Hosp, Baylor Coll Med, Dept Pediat Med GI Hepatol & Nutr, Houston, TX 77030 USA. [Huang, Terry] Univ Nebraska Med Ctr, Dept Hlth Promot, Omaha, NE USA. [Finkelstein, Eric] Duke Univ Global Hlth Inst, Program Hlth Serv Syst Res, Singapore, Singapore. [Pont, Stephen] Univ Texas Southwestern Austin, Texas Ctr Prevent & Treatment Childhood Obes, Dell Childrens Med Ctr, Austin, TX USA. [Sacher, Paul] UCL, Childhood Nutr Res Ctr, London, England. [Sharma, Shreela V.; Durand, Casey] Univ Texas Sch Publ Hlth, Michael & Susan Dell Ctr Hlth Living, Houston, TX USA. RP Hoelscher, DM (reprint author), Univ Texas Sch Publ Hlth, Michael & Susan Dell Ctr Hlth Living, Austin Reg Campus 1616 Guadalupe,Suite 6-300, Austin, TX 78701 USA. EM Deanna.M.Hoelscher@uth.tmc.edu FU CDC [RFA-DP-11-007]; USDA/ARS [58-6250-0-008] FX This research was supported by cooperative agreement RFA-DP-11-007 from the CDC. The content is solely the responsibility of the authors and does not necessarily represent the official views of the CDC. Additional support was provided by the Michael and Susan Dell Foundation through the Michael & Susan Dell Center for Healthy Living. This work is a publication of the USDA (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, and had been funded, in part, with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. NR 67 TC 7 Z9 7 U1 3 U2 29 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD FEB 1 PY 2015 VL 11 IS 1 BP 71 EP 91 DI 10.1089/chi.2014.0084 PG 21 WC Pediatrics SC Pediatrics GA CB0LH UT WOS:000349317000008 PM 25555188 ER PT J AU O'Connor, DP Lee, RE Mehta, P Thompson, D Bhargava, A Carlson, C Kao, D Layne, CS Ledoux, T O'Connor, T Rifai, H Gulley, L Hallett, AM Kudia, O Joseph, S Modelska, M Ortega, D Parker, N Stevens, A AF O'Connor, Daniel P. Lee, Rebecca E. Mehta, Paras Thompson, Debbe Bhargava, Alok Carlson, Coleen Kao, Dennis Layne, Charles S. Ledoux, Tracey O'Connor, Teresia Rifai, Hanadi Gulley, Lauren Hallett, Allen M. Kudia, Ousswa Joseph, Sitara Modelska, Maria Ortega, Dana Parker, Nathan Stevens, Andria CA EC-CORD BETTER Policies Team TI Childhood Obesity Research Demonstration Project: Cross-Site Evaluation Methods SO CHILDHOOD OBESITY LA English DT Article ID HEALTH; QUESTIONNAIRE; INTERVENTION; VALIDITY; FEASIBILITY; RELIABILITY; EDUCATION; PROGRAMS; LESSONS; POLICY AB Introduction: The Childhood Obesity Research Demonstration (CORD) project links public health and primary care interventions in three projects described in detail in accompanying articles in this issue of Childhood Obesity. This article describes a comprehensive evaluation plan to determine the extent to which the CORD model is associated with changes in behavior, body weight, BMI, quality of life, and healthcare satisfaction in children 2-12 years of age. Design/Methods: The CORD Evaluation Center (EC-CORD) will analyze the pooled data from three independent demonstration projects that each integrate public health and primary care childhood obesity interventions. An extensive set of common measures at the family, facility, and community levels were defined by consensus among the CORD projects and EC-CORD. Process evaluation will assess reach, dose delivered, and fidelity of intervention components. Impact evaluation will use a mixed linear models approach to account for heterogeneity among project-site populations and interventions. Sustainability evaluation will assess the potential for replicability, continuation of benefits beyond the funding period, institutionalization of the intervention activities, and community capacity to support ongoing program delivery. Finally, cost analyses will assess how much benefit can potentially be gained per dollar invested in programs based on the CORD model. Conclusions: The keys to combining and analyzing data across multiple projects include the CORD model framework and common measures for the behavioral and health outcomes along with important covariates at the individual, setting, and community levels. The overall objective of the comprehensive evaluation will develop evidence-based recommendations for replicating and disseminating community-wide, integrated public health and primary care programs based on the CORD model. C1 [O'Connor, Daniel P.; Layne, Charles S.; Ledoux, Tracey; Gulley, Lauren; Hallett, Allen M.; Kudia, Ousswa; Joseph, Sitara; Ortega, Dana; Parker, Nathan; Stevens, Andria] Univ Houston, Dept Hlth & Human Performance, Texas Obes Res Ctr, Houston, TX 77204 USA. [Lee, Rebecca E.] Arizona State Univ, Coll Nursing & Hlth Innovat, Phoenix, AZ USA. [Mehta, Paras; Carlson, Coleen] Univ Houston, Dept Psychol, Texas Inst Measurement, Houston, TX USA. [Thompson, Debbe; O'Connor, Teresia] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Bhargava, Alok] Univ Maryland, Sch Publ Policy, College Pk, MD 20742 USA. [Kao, Dennis] Univ Houston, Grad Coll Social Work, Houston, TX USA. [Rifai, Hanadi; Modelska, Maria] Univ Houston, Dept Civil & Environm Engn, Houston, TX 77204 USA. RP O'Connor, DP (reprint author), Univ Houston, Dept Hlth & Human Performance, Texas Obes Res Ctr, 3855 Holman GAR104, Houston, TX 77204 USA. EM dpoconno@central.uh.edu OI Carlson, Coleen/0000-0001-8050-5671 FU CDC [RFA-DP-11-007, U18DP003350] FX This research was supported, in part, by cooperative agreement RFA-DP-11-007 (grant U18DP003350) from the CDC. The content is solely the responsibility of the authors and does not necessarily represent the official views of the CDC. NR 48 TC 9 Z9 9 U1 2 U2 7 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2153-2168 EI 2153-2176 J9 CHILD OBES JI Child Obes. PD FEB 1 PY 2015 VL 11 IS 1 BP 92 EP 103 DI 10.1089/chi.2014.0061 PG 12 WC Pediatrics SC Pediatrics GA CB0LH UT WOS:000349317000009 PM 25679060 ER PT J AU Wang, LY Ware, D Lushbough, C Merchant, N Stein, L AF Wang, Liya Ware, Doreen Lushbough, Carol Merchant, Nirav Stein, Lincoln TI A genome-wide association study platform built on iPlant cyber-infrastructure SO CONCURRENCY AND COMPUTATION-PRACTICE & EXPERIENCE LA English DT Article; Proceedings Paper CT International Workshop on Science Gateways CY JUN 03-05, 2013 CL Zurich, SWITZERLAND DE GWAS; iPlant Data Store; iPlant Foundation API; iPlant Discovery Environment; BioExtract server ID QUANTITATIVE TRAIT LOCI; POPULATION-STRUCTURE; COMPLEX TRAITS; MODEL APPROACH; SEQUENCES; SOFTWARE AB We demonstrate a flexible genome-wide association study platform built upon the iPlant Collaborative Cyber-infrastructure. The platform supports big data management, sharing, and large-scale study of both genotype and phenotype data on clusters. End users can add their own analysis tools and create customized analysis workflows through the graphical user interfaces in both iPlant Discovery Environment and BioExtract server. Copyright (c) 2014 John Wiley & Sons, Ltd. C1 [Wang, Liya; Ware, Doreen] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Ware, Doreen] USDA, Cold Spring Harbor, NY USA. [Lushbough, Carol] Univ S Dakota, Vermillion, SD 57069 USA. [Merchant, Nirav] Univ Arizona, Tucson, AZ USA. [Stein, Lincoln] Ontario Inst Canc Res, Toronto, ON, Canada. [Stein, Lincoln] Univ Toronto, Toronto, ON, Canada. EM Liya.Wang@cshl.edu FU National Science Foundation [DBI-0735191]; USDA-ARS FX This work is supported by the National Science Foundation Plant Cyber-infrastructure Program(#DBI-0735191 and #DBI-) and USDA-ARS. The authors would like to thank the two anonymous reviewers for their helpful comments. L. W. would like to thank Drs. Joshua Stein and Stephen Goff for critical reading of the paper, Drs. Peter Bradbury and Ed Buckler for the early collaboration on integrating TASSEL, Terry Casstevens for collaboratively developing the command line plugins for the TASSEL suites (basis for integrating TASSEL into iPlant CI), Dr. Qi Sun for helping on integrating GBS workflow and analyzing sorghum data, Dr. Kang for helping on EMMAX integration, Dr. Geoffrey Morris for assisting with extracting the sorghum data, Dr. Ivan Baxter and Greg Ziegler for the NAM bootstrapping collaboration, Drs. Rion Dooley, Matthew Vaughn, and Paul Navratil for assistances on deploying apps on TACC Lonestar and Longhorn clusters. NR 33 TC 2 Z9 2 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1532-0626 EI 1532-0634 J9 CONCURR COMP-PRACT E JI Concurr. Comput.-Pract. Exp. PD FEB PY 2015 VL 27 IS 2 SI SI BP 420 EP 432 DI 10.1002/cpe.3236 PG 13 WC Computer Science, Software Engineering; Computer Science, Theory & Methods SC Computer Science GA CA7HG UT WOS:000349087800012 ER PT J AU Chen, GQ Ren, L Zhang, J Reed, BM Zhang, D Shen, XH AF Chen, Guan-qun Ren, Li Zhang, Jie Reed, Barbara M. Zhang, Di Shen, Xiao-hui TI Cryopreservation affects ROS-induced oxidative stress and antioxidant response in Arabidopsis seedlings SO CRYOBIOLOGY LA English DT Article DE Arabidopsis seedlings; Vitrification-based cryopreservation; ROS; Oxidative stress response; Antioxidant systems ID EUGLENA-GRACILIS; TISSUE CULTURES; SOMATIC EMBRYOS; GENE-EXPRESSION; CELL-DEATH; OXYGEN; PLANTS; SUPEROXIDE; ACID; PEROXIDATION AB Plant recovery status after cryopreservation by vitrification had a negative relationship to the oxidative stress induced by reactive oxygen species (ROS). Arabidopsis thaliana seedlings germinated for 48 h or 72 h with different survival tolerances were examined at five steps of cryopreservation, to determine the role of ROS H2O2 and OW) and antioxidant systems (SOD, POD, CAT, AsA and GSH) in cryo-injury. In addition, the effects of the steps on membrane lipid peroxidation were studied using malondialdehyde (MDA) as an indicator. The results indicated that H2O2-induced oxidative stress at the steps of dehydration and rapid warming was the main cause of cryo-injury of 48-h seedlings (high survival rate) and 72-h seedlings (no survival). The H2O2 was mainly generated in cotyledons, shoot tips and roots of seedlings as indicated by Amplex Red staining. Low survival of 72-h seedlings was associated with severe membrane lipid peroxidation, which was caused by increased OH. generation activity and decreased SOD activity. The antioxidant-related gene expression by qRT-PCR and physiological assays suggested that the antioxidant system of 48-h seedlings were activated by ROS, and they mounted a defense against oxidative stress. A high level of ROS led to the Weakening of the antioxidant system of 72-h seedlings. Correlation analysis indicated that enhanced antioxidant enzymes activities contributed to the high survival rate of 48-h seedlings, which could reflect by cryopreservation of antioxidant mutant seedlings. This model system indicated that elevated CAT activity and AsA content were determinants of cryogenic stress tolerance, whose manipulation could improve the recovery of seedlings after cryopreservation. (C) 2014 Elsevier Inc. All rights reserved. C1 [Chen, Guan-qun; Ren, Li; Zhang, Jie; Zhang, Di; Shen, Xiao-hui] Shanghai Jiao Tong Univ, Sch Agr & Biol, Key Lab Urban Agr South, Minist Agr, Shanghai 200240, Peoples R China. [Reed, Barbara M.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Shen, XH (reprint author), Shanghai Jiao Tong Univ, Sch Agr & Biol, Key Lab Urban Agr South, Minist Agr, 800 Rd Dong Chuan, Shanghai 200240, Peoples R China. EM chengq0111@gmail.com; renliaqx@gmail.com; zjjsnake@sina.com; Barbara.Reed@ars.usda.gov; zhangdi2013@sjtu.edu.cn; shenxh62@sjtu.edu.cn FU National Natural Science Funding of China [31170655, 31300580]; Program of Technology Innovation from Shanghai Municipal Education Commission [14ZZ029]; Shanghai Graduate Education and Innovation Program (Horticulture) FX (1) National Natural Science Funding of China (No. 31170655). (2) National Natural Science Funding of China (No. 31300580). (3) The Program of Technology Innovation (No. 14ZZ029) from Shanghai Municipal Education Commission. (4) Shanghai Graduate Education and Innovation Program (Horticulture). NR 59 TC 9 Z9 10 U1 6 U2 63 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0011-2240 EI 1090-2392 J9 CRYOBIOLOGY JI Cryobiology PD FEB PY 2015 VL 70 IS 1 BP 38 EP 47 DI 10.1016/j.cryobiol.2014.11.004 PG 10 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA CA0UN UT WOS:000348631900006 PM 25489814 ER PT J AU Hobson, EA John, DJ Mcintosh, TL Avery, ML Wright, TF AF Hobson, Elizabeth A. John, Darlene J. Mcintosh, Tiffany L. Avery, Michael L. Wright, Timothy F. TI The effect of social context and social scale on the perception of relationships in monk parakeets SO CURRENT ZOOLOGY LA English DT Article DE Affiliative; Agonistic; Dominance; Eigenvector centrality; Emergent social property; Myiopsitta monachus; Parrot; Popularity; Rank; Social network analysis ID BUDGERIGARS MELOPSITTACUS-UNDULATUS; DOMINANCE NETWORK STRUCTURE; CONTACT CALLS; HIERARCHY FORMATION; FEMALE PRIMATES; BABOONS; RANK; DYNAMICS; RECOGNITION; MACAQUES AB Social relationships formed within a network of interacting group members can have a profound impact on an individual's behavior and fitness. However, we have little understanding of how individuals perceive their relationships and how this perception relates to our external measures of interactions. We investigated the perception of affiliative and agonistic relationships at both the dyadic and emergent social levels in two captive groups of monk parakeets (Myiopsitta monachus, n = 21 and 19) using social network analysis and playback experiments. At the dyadic social scale, individuals directed less aggression towards their strong affiliative partners and more aggression towards non-partner neighbors. At the emergent social scale, there was no association between relationships in different social contexts and an individual's dominance rank did not correlate with its popularity rank. Playback response patterns were mainly driven by relationships in affiliative social contexts at the dyadic scale. In both groups, individual responses to playback experiments were significantly affected by strong affiliative relationships at the dyadic social scale, albeit in different directions in the two groups. Response patterns were also affected by affiliative relationships at the emergent social scale, but only in one of the two groups. Within affiliative relationships, those at the dyadic social scale were perceived by individuals in both groups, but those at the emergent social scale only affected responses in one group. These results provide preliminary evidence that relationships in affiliative social contexts may be perceived as more important than agonistic relationships in captive monk parakeet groups. Our approach could be used in a wide range of social species and comparative analyses could provide important insight into how individuals perceive relationships across social contexts and social scales C1 [Hobson, Elizabeth A.] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. [Hobson, Elizabeth A.; John, Darlene J.; Mcintosh, Tiffany L.; Wright, Timothy F.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Avery, Michael L.] US Fish & Wildlife Serv, USDA, Natl Wildlife Res Ctr, Gainesville, FL 32641 USA. RP Hobson, EA (reprint author), Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. EM ehobson@nimbios.org FU New Mexico Higher Education Graduate fellowship; Loustaunau Fellowship from NMSU; NSF GK-12 DISSECT Fellowship [DGE-0947465]; New Mexico State University; American Ornithologists' Union; Sigma Xi; NMSU Biology Graduate Student Organization; NSF [IOS-0725032]; REU; National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF [EF-0832858, DBI-1300426]; University of Tennessee, Knoxville FX We thank the staff of the USDA/NWRC Florida Field Station for their support, especially Kandy Keacher for assistance in animal husbandry and data record-keeping. We also thank A. Hobson for help with data collection. Comments from W. Boecklen, A. Ilany, A. Kershenbaum, members of the Wright, Mabry, and Freeberg labs, and three anonymous reviewers significantly improved this manuscript. This study was funded by a New Mexico Higher Education Graduate fellowship, a Loustaunau Fellowship from NMSU, and a NSF GK-12 DISSECT (#DGE-0947465) Fellowship to E.A.H., research grants from the Associated Students of New Mexico State University, American Ornithologists' Union, Sigma Xi, and the NMSU Biology Graduate Student Organization to E.A.H., and NSF #IOS-0725032 and associated REU supplement to T.F.W. A portion of this work was conducted while E.A.H. was a Postdoctoral Fellow at the National Institute for Mathematical and Biological Synthesis, an Institute sponsored by the National Science Foundation, the U.S. Department of Homeland Security, and the U.S. Department of Agriculture through NSF Awards #EF-0832858 and #DBI-1300426, with additional support from the University of Tennessee, Knoxville. NR 83 TC 3 Z9 3 U1 12 U2 39 PU CURRENT ZOOLOGY PI BEIJING PA CHINESE ACAD SCIENCES, INST ZOOLOGY, BEICHEN XILU, CHAOYANG DISTRICT, BEIJING, 100101, PEOPLES R CHINA SN 1674-5507 J9 CURR ZOOL JI Curr. Zool. PD FEB PY 2015 VL 61 IS 1 BP 55 EP 69 PG 15 WC Zoology SC Zoology GA CA6EZ UT WOS:000349003500007 ER PT J AU Gese, EM Knowlton, FF Adams, JR Beck, K Fuller, TK Murray, DL Steury, TD Stoskopf, MK Waddell, WT Waits, LP AF Gese, Eric M. Knowlton, Fred F. Adams, Jennifer R. Beck, Karen Fuller, Todd K. Murray, Dennis L. Steury, Todd D. Stoskopf, Michael K. Waddell, Will T. Waits, Lisette P. TI Managing hybridization of a recovering endangered species: The red wolf Canis rufus as a case study SO CURRENT ZOOLOGY LA English DT Article DE Canid; Conservation; Genetics; Hybrid; Management ID KERNEL DENSITY-ESTIMATION; SELECTORS; WOLVES AB Hybridization presents a unique challenge for conservation biologists and managers. While hybridization is an important evolutionary process, hybridization is also a threat formany native species. The endangered species recovery effort for the red wolf Canis rufus is a classic system for understanding and addressing the challenges of hybridization. From 1987. 1993, 63 red wolves were released from captivity in eastern North Carolina, USA, to establish a free-ranging, non-essential experimental population. By 1999, managers recognized hybridization with invasive coyotes Canis latrans was the single greatest threat to successful recovery, and an adaptive management plan was adopted with innovative approaches for managing the threat of hybridization. Here we review the application and results of the adaptive management efforts from 1993 to 2013 by comparing: (1) the numbers of wolves, coyotes, and hybrids captured, (2) the numbers of territorial social groups with presumed breeding capabilities, (3) the number of red wolf and hybrid litters documented each year and (4) the degree of coyote introgression into the wild red wolf gene pool. We documented substantial increases in the number of known red wolves and red wolf social groups from 1987-2004 followed by a plateau and slight decline by 2013. The number of red wolf litters exceeded hybrid litters each year and the proportion of hybrid litters per year averaged 21%. The genetic composition of the wild red wolf population is estimated to include <4% coyote ancestry from recent introgression since reintroduction. We conclude that the adaptive management plan was effective at reducing the introgression of coyote genes into the red wolf population, but population recovery of red wolves will require continuation of the current management plan, or alternative approaches, for the foreseeable future. More broadly, we discuss the lessons learned from red wolf adaptive management that could assist other endangered species recovery efforts facing the challenge of minimizing hybridization C1 [Gese, Eric M.; Knowlton, Fred F.] Utah State Univ, USDA APHIS WS Natl Wildlife Res Ctr, Dept Wildland Resources, Logan, UT 84322 USA. [Adams, Jennifer R.; Waits, Lisette P.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Beck, Karen; Stoskopf, Michael K.] N Carolina State Univ, Coll Vet Med, Environm Med Consortium, Raleigh, NC 27606 USA. [Fuller, Todd K.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Murray, Dennis L.] Trent Univ, Dept Biol, Peterborough, ON K9J 7B8, Canada. [Steury, Todd D.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Waddell, Will T.] Point Defiance Zoo & Aquarium, Tacoma, WA 98407 USA. RP Waits, LP (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. EM lwaits@uidaho.edu RI Gese, Eric/B-4578-2011 NR 10 TC 15 Z9 15 U1 33 U2 142 PU CURRENT ZOOLOGY PI BEIJING PA CHINESE ACAD SCIENCES, INST ZOOLOGY, BEICHEN XILU, CHAOYANG DISTRICT, BEIJING, 100101, PEOPLES R CHINA SN 1674-5507 J9 CURR ZOOL JI Curr. Zool. PD FEB PY 2015 VL 61 IS 1 BP 191 EP 205 PG 15 WC Zoology SC Zoology GA CA6EZ UT WOS:000349003500018 ER PT J AU Allen, LR Engeman, RM AF Allen, Lee R. Engeman, Richard M. TI Evaluating and validating abundance monitoring methods in the absence of populations of known size: review and application to a passive tracking index SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Activity index; Animal surveys; Model sensitivity; Predators; Trends; Validation ID NEW-SOUTH-WALES; INVASIVE MESOPREDATOR; CENTRAL AUSTRALIA; TOP-PREDATOR; BASICS RIGHT; CARNIVORES; DINGOES; HABITAT; MAMMALS; MODELS AB Rarely is it possible to obtain absolute numbers in free-ranging populations and although various direct and indirect methods are used to estimate abundance, few are validated against populations of known size. In this paper, we apply grounding, calibration and verification methods, used to validate mathematical models, to methods of estimating relative abundance. To illustrate how this might be done, we consider and evaluate the widely applied passive tracking index (PTI) methodology. Using published data, we examine the rationality of PTI methodology, how conceptually animal activity and abundance are related and how alternative methods are subject to similar biases or produce similar abundance estimates and trends. We then attune the method against populations representing a range of densities likely to be encountered in the field. Finally, we compare PTI trends against a prediction that adjacent populations of the same species will have similar abundance values and trends in activity. We show that while PTI abundance estimates are subject to environmental and behavioural stochasticity peculiar to each species, the PTI method and associated variance estimate showed high probability of detection, high precision of abundance values and, generally, low variability between surveys, and suggest that the PTI method applied using this procedure and for these species provides a sensitive and credible index of abundance. This same or similar validation approach can and should be applied to alternative relative abundance methods in order to demonstrate their credibility and justify their use. C1 [Allen, Lee R.] Robert Wicks Pest Anim Res Ctr, Dept Agr Fisheries & Forestry, Toowoomba, Qld 4350, Australia. [Engeman, Richard M.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Allen, LR (reprint author), Robert Wicks Pest Anim Res Ctr, Dept Agr Fisheries & Forestry, POB 102, Toowoomba, Qld 4350, Australia. EM Lee.Allen@daff.qld.gov.au NR 52 TC 5 Z9 5 U1 3 U2 16 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD FEB PY 2015 VL 22 IS 4 BP 2907 EP 2915 DI 10.1007/s11356-014-3567-3 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA CB0MC UT WOS:000349319200054 PM 25223358 ER PT J AU Tseng, M Fratamico, PM Bagi, L Manzinger, D Funk, JA AF Tseng, M. Fratamico, P. M. Bagi, L. Manzinger, D. Funk, J. A. TI Shiga toxin-producing E. coli (STEC) in swine: prevalence over the finishing period and characteristics of the STEC isolates SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Epidemiology; longitudinal; serotype; Shiga-like toxin-producing E. coli; swine ID HEMOLYTIC-UREMIC-SYNDROME; ESCHERICHIA-COLI; CHROMAGAR STEC; STRAINS; OUTBREAK; O157-H7; NON-O157; GENES; O157H7; CANADA AB This descriptive longitudinal study was conducted to investigate the faecal shedding of Shiga toxin-producing E. coli (STEC) in finishing swine and to characterize the swine STEC isolates that were recovered. Three cohorts of finishing swine (n = 50/cohort; total 150 pigs) were included in the longitudinal study. Individual faecal samples were collected every 2 weeks (8 collections/ pig) from the beginning (pig age 10 weeks) to the end (pig age 24 weeks) of the finishing period. STEC isolates were recovered in at least one sample from 65.3% (98/150) of the pigs, and the frequency distribution of first-time STEC detection during the finishing period resembled a point-source outbreak curve. Nineteen O: H serotypes were identified among the STEC isolates. Most STEC isolates (n = 148) belonged to serotype O59: H21 and carried the stx2e gene. One O49: H21 STEC isolate carried the stx2e and eae genes. High prevalence rates of STEC during the finishing period were observed, and STEC isolates in various non-O157 serogroups were recovered. These data enhance understanding of swine STEC epidemiology, and future research is needed to confirm whether or not swine STEC are of public health concern. C1 [Tseng, M.; Funk, J. A.] Michigan State Univ, Coll Vet Med, E Lansing, MI 48824 USA. [Fratamico, P. M.; Bagi, L.; Manzinger, D.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA. RP Tseng, M (reprint author), Food Safety & Toxicol Bldg,1129 Farm Lane Rm B41, E Lansing, MI 48824 USA. EM marionkttseng@gmail.com FU National Pork Board [12-069] FX This work was supported by the National Pork Board (project no. 12-069). NR 40 TC 3 Z9 3 U1 0 U2 11 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD FEB PY 2015 VL 143 IS 3 BP 505 EP 514 DI 10.1017/S0950268814001095 PG 10 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA CA1BC UT WOS:000348646700007 PM 24810787 ER PT J AU Bartuska, A AF Bartuska, Ann TI Beyond desertification SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Editorial Material C1 USDA, Washington, DC 20250 USA. RP Bartuska, A (reprint author), USDA, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 3 U2 15 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2015 VL 13 IS 1 SI SI BP 3 EP 3 PG 1 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA5UT UT WOS:000348974000001 ER PT J AU Peters, DPC Havstad, KM Archer, SR Sala, OE AF Peters, Debra P. C. Havstad, Kris M. Archer, Steven R. Sala, Osvaldo E. TI Beyond desertification: new paradigms for dryland landscapes SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID SOUTHERN NEW-MEXICO; GLOBAL DESERTIFICATION; UNITED-STATES; ECOSYSTEMS; VEGETATION; ECOLOGY; SCIENCE; CLIMATE; SYSTEMS; SHIFTS AB The traditional desertification paradigm focuses on the losses of ecosystem services that typically occur when grasslands transition to systems dominated by bare (unvegetated) ground or by woody plants that are unpalatable to domestic livestock. However, recent studies reveal complex transitions across a range of environmental conditions and socioeconomic contexts. The papers in this Special Issue illustrate how an improved understanding of these dynamics is generating more robust paradigms, where state changes and regime shifts occurring within the context of changes in land use and climate are modified by landform and antecedent conditions. New and emerging technologies are being used to characterize and evaluate processes and outcomes across various scales and levels of organization. At the same time, developments in education are taking advantage of these new perspectives. to improve the ecological literacy of future generations, and to better inform land-management decisions. A framework that integrates these perspectives provides a more comprehensive approach for understanding and predicting dryland dynamics. C1 [Peters, Debra P. C.; Havstad, Kris M.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. [Archer, Steven R.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Sala, Osvaldo E.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Sala, Osvaldo E.] Arizona State Univ, Sch Sustainabil, Tempe, AZ USA. RP Peters, DPC (reprint author), USDA, Off Chief Scientist, Washington, DC 20250 USA. EM debpeter@nmsu.edu FU NSF through New Mexico State University [DEB-1235828]; USDA-ARS [6235-11210-007]; Arizona Agricultural Experiment Station [ARZT- 1 360540-H1 2- 99] FX Funding was provided by the NSF to the Jornada Basin Long Term Ecological Research Program through New Mexico State University (DEB-1235828). DPCP and KMH acknowledge the USDA-ARS (#6235-11210-007) for ongoing support; SRA acknowledges support from the Arizona Agricultural Experiment Station (ARZT- 1 360540-H1 2- 99). NR 49 TC 9 Z9 9 U1 8 U2 74 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2015 VL 13 IS 1 SI SI BP 4 EP 12 DI 10.1890/140276 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA5UT UT WOS:000348974000002 ER PT J AU Okin, GS Moreno-de las Heras, M Saco, PM Throop, HL Vivoni, ER Parsons, AJ Wainwright, J Peters, DPC AF Okin, Gregory S. Moreno-de las Heras, Mariano Saco, Patricia M. Throop, Heather L. Vivoni, Enrique R. Parsons, Anthony J. Wainwright, John Peters, Debra P. C. TI Connectivity in dryland landscapes: shifting concepts of spatial interactions SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID PLANT-SOIL INTERACTIONS; SEMIARID LANDSCAPES; HYDROLOGICAL CONNECTIVITY; GLOBAL DESERTIFICATION; VEGETATION PATCHES; LAND DEGRADATION; WIND EROSION; SCALE; PATTERNS; ECOSYSTEMS AB Dry land ecosystems are often characterized by patchy vegetation and exposed soil. This structure enhances transport of soil resources and seeds through the landscape (primarily by wind and water, but also by animals), thus emphasizing the importance of connectivity - given its relation to the flow of these materials - as a component of dryland ecosystem function. We argue that, as with the fertile-islands conceptual model before it, the concept of connectivity explains many phenomena observed in drylands. Further, it-serves as an organizing-principle-to understand dryland structure and function at scales from individual plants to entire landscapes. The concept of connectivity also helps to organize thinking about interactions among processes occurring at different scales, such as when processes at one scale are overridden by processes at another. In these cases, we suggest that state change occurs when fine-scale processes fail to adjust to new external conditions through resource use or redistribution at the finer scale. The connectivity framework has practical implications for land management, especially with respect to decision making concerning the scale and location of agricultural production or habitat restoration in the world's drylands. C1 [Okin, Gregory S.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. [Moreno-de las Heras, Mariano; Wainwright, John] Univ Durham, Dept Geog, Durham, England. [Saco, Patricia M.] Univ Newcastle, Sch Engn, Callaghan, NSW 2308, Australia. [Throop, Heather L.] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA. [Vivoni, Enrique R.] Arizona State Univ, Sch Earth & Space Explorat, Tempe, AZ USA. [Parsons, Anthony J.] Univ Sheffield, Dept Geog, Sheffield S10 2TN, S Yorkshire, England. [Peters, Debra P. C.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. RP Okin, GS (reprint author), Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. EM okin@ucla.edu RI Vivoni, Enrique/E-1202-2012; Moreno de las Heras, Mariano/M-2542-2013; Throop, Heather/D-6391-2012; OI Vivoni, Enrique/0000-0002-2659-9459; Moreno de las Heras, Mariano/0000-0003-3366-5060; Throop, Heather/0000-0002-7963-4342; parsons, anthony/0000-0002-6730-5031 FU Jornada Basin Long Term Ecological Research Program [DEB-1235828]; USDA-ARS Jornada Experimental Range; NSF [EAR-1148334]; Marie Curie fellowship - European Commission (VEGDESERT) [PIEF-GA-2012-329298] FX This contribution was supported by Jornada Basin Long Term Ecological Research Program (DEB-1235828), USDA-ARS Jornada Experimental Range, NSF Grant EAR-1148334, and a Marie Curie fellowship funded by the European Commission (VEGDESERT, PIEF-GA-2012-329298). NR 56 TC 14 Z9 15 U1 9 U2 53 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2015 VL 13 IS 1 SI SI BP 20 EP 27 DI 10.1890/140163 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA5UT UT WOS:000348974000004 ER PT J AU Bestelmeyer, BT Okin, GS Duniway, MC Archer, SR Sayre, NF Williamson, JC Herrick, JE AF Bestelmeyer, Brandon T. Okin, Gregory S. Duniway, Michael C. Archer, Steven R. Sayre, Nathan F. Williamson, Jebediah C. Herrick, Jeffrey E. TI Desertification, land use, and the transformation of global drylands SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID VEGETATION DYNAMICS; SEMIARID GRASSLAND; TRANSITION MODELS; SOIL QUALITY; COVER CHANGE; RANGELANDS; MANAGEMENT; SCIENCE; STATE; 21ST-CENTURY AB Desertification is an escalating concern in global drylands, yet assessments to guide management and policy responses are limited by ambiguity concerning the definition of "desertification" and what processes are involved. To improve clarity, we propose that assessments of desertification and land transformation be placed within a state change-land-use change (SC-LUC) framework. This framework considers desertification as state changes occurring within the context of particular land uses (eg rangeland, cropland) That interact with land-use change. State changes that can be readily reversed are distinguished from regime shifts, which are state changes involving persistent alterations to vegetation or soil properties. Pressures driving the transformation of rangelands to other types of land uses may be low, fluctuating, or high, and may influence and be influenced by state change. We discuss how the SC-LUC perspective can guide more effective assessment of desertification and management of drylands. C1 [Bestelmeyer, Brandon T.; Williamson, Jebediah C.; Herrick, Jeffrey E.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. [Okin, Gregory S.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. [Duniway, Michael C.] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT USA. [Archer, Steven R.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Sayre, Nathan F.] Univ Calif Berkeley, Dept Geog, Berkeley, CA 94720 USA. RP Bestelmeyer, BT (reprint author), New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. EM bbestelm@nmsu.edu OI Duniway, Michael/0000-0002-9643-2785 FU Jornada Basin Long Term Ecological Research Program [DEB-1235828]; USDA-ARS Jornada Experimental Range; Arizona Agricultural Experiment Station [ARZT- 1 360540-H1 2- 99] FX This contribution was supported by Jornada Basin Long Term Ecological Research Program (DEB-1235828) and the USDA-ARS Jornada Experimental Range. SRA acknowledges support from the Arizona Agricultural Experiment Station (ARZT- 1 360540-H1 2- 99). We thank DPC Peters for comments that improved the paper. NR 62 TC 23 Z9 23 U1 12 U2 75 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2015 VL 13 IS 1 SI SI BP 28 EP 36 DI 10.1890/140162 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA5UT UT WOS:000348974000005 ER PT J AU Yahdjian, L Sala, OE Havstad, KM AF Yahdjian, Laura Sala, Osvaldo E. Havstad, Kris M. TI Rangeland ecosystem services: shifting focus from supply to reconciling supply and demand SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID LIVESTOCK PRODUCTION; SYSTEMS; SCIENCE AB Ecosystem services have been extensively studied in recent decades. Most of the thousands of scholarly papers published on the subject have focused on describing the production, spatial extent, and valuation of such services. Human reliance on ecosystem services is a function of ecosystems' capacity to supply and societal demand for these benefits. However, considerably more attention has been devoted to the supply side than to the demand for them. Sustainable land management depends on reconciling supply of and demand for ecosystem services among different stakeholders. The emphasis is now shifting from the supply of ecosystem services to attaining a balance between supply and demand. Here, we illustrate the demand for rangeland ecosystem services, describe current changes in societal demand, and present a specific provisioning service to exemplify the dynamic nature of reconciling ecosystem-service supply and demand. C1 [Yahdjian, Laura] Univ Buenos Aires, Fac Agron, Inst Agr Plant Physiol & Ecol IFEVA, Buenos Aires, DF, Argentina. [Yahdjian, Laura] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Sala, Osvaldo E.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Sala, Osvaldo E.] Arizona State Univ, Sch Sustainabil, Tempe, AZ USA. [Havstad, Kris M.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. RP Yahdjian, L (reprint author), Univ Buenos Aires, Fac Agron, Inst Agr Plant Physiol & Ecol IFEVA, Buenos Aires, DF, Argentina. EM yahdjian@ifeva.edu.ar FU National Academies Keck Futures Initiative [025512]; NSF [DEB-1242747, DEB 1235828]; USDA-ARS CRIS [6235-11210-007D]; Argentinean National Research Council (CONICET) [PIP 112-201101-00555, PICT 2010-0553] FX We thank J Yao for assistance in data gathering. This work has been supported by the National Academies Keck Futures Initiative award 025512 (NAKFI), by NSF DEB-1242747 and DEB 1235828, and by USDA-ARS CRIS Project 6235-11210-007D. LY was supported by the Argentinean National Research Council (CONICET, PIP 112-201101-00555 and PICT 2010-0553). NR 31 TC 10 Z9 12 U1 8 U2 36 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2015 VL 13 IS 1 SI SI BP 44 EP 51 DI 10.1890/140156 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA5UT UT WOS:000348974000007 ER PT J AU Browning, DM Rango, A Karl, JW Laney, CM Vivoni, ER Tweedie, CE AF Browning, Dawn M. Rango, Albert Karl, Jason W. Laney, Christine M. Vivoni, Enrique R. Tweedie, Craig E. TI Emerging technological and cultural shifts advancing drylands research and management SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID CROSS-SCALE INTERACTIONS; SENSOR NETWORKS; SYSTEM DYNAMICS; LAND-USE; ECOLOGY; SCIENCE; RANGELAND; CHALLENGES; KNOWLEDGE; ECOSYSTEM AB Sustainable management of arid landscapes is complicated by extreme conditions that constrain biological responses to perturbation, great spatial complexity, and uncertain degrees of ecosystem resilience to climate change. Traditional approaches to the collection, management, and analysis of data from dryland monitoring efforts should consider these complications. Over the past century, research on drylands has gradually transitioned from short-term, plot-scale studies to long-term, regional- and biome-scale efforts. Two thresholds are imminent: a technological tipping point that will facilitate performing novel science using new techniques to collect, manage, and analyze data, and a cultural tipping point, where various research products are shared more freely and through different communication pathways. A new framework could be developed by promoting interdisciplinary collaboration and implementing standardized practices regarding data collection, curation, and sharing. C1 [Browning, Dawn M.; Rango, Albert; Karl, Jason W.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. [Laney, Christine M.; Tweedie, Craig E.] Univ Texas El Paso, Dept Biol Sci, El Paso, TX 79968 USA. [Vivoni, Enrique R.] Arizona State Univ, Sch Earth & Space Explorat, Tempe, AZ USA. [Vivoni, Enrique R.] Arizona State Univ, Sch Sustainable Engn & Built Environm, Tempe, AZ USA. RP Browning, DM (reprint author), New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. EM dbrownin@nmsu.edu RI Vivoni, Enrique/E-1202-2012; Laney, Christine/B-7867-2010 OI Vivoni, Enrique/0000-0002-2659-9459; Laney, Christine/0000-0002-4944-2083 FU US National Science Foundation (NSF) through New Mexico State University [DEB-1235828]; Cyber-ShARE Center of Excellence at the University of Texas at El Paso [HRD-1242122]; Army Research Office [56059-EV-PCS]; USDA-ARS CRIS [6235-11210-007] FX Funding was provided by the US National Science Foundation (NSF) to the Jornada Basin Long Term Ecological Research Program through New Mexico State University (DEB-1235828), the Cyber-ShARE Center of Excellence at the University of Texas at El Paso (HRD-1242122), the Army Research Office (56059-EV-PCS), and USDA-ARS CRIS Project # 6235-11210-007. R Wu assisted with graphics in Figures 1 and 4. Any opinions, findings, conclusions, or recommendations expressed in this paper are those of the authors and do not necessarily reflect the views of the NSF. NR 57 TC 5 Z9 5 U1 2 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD FEB PY 2015 VL 13 IS 1 SI SI BP 52 EP 60 DI 10.1890/140161 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA CA5UT UT WOS:000348974000008 ER PT J AU Anderson-Teixeira, KJ Davies, SJ Bennett, AC Gonzalez-Akre, EB Muller-Landau, HC Wright, SJ Abu Salim, K Zambrano, AMA Alonso, A Baltzer, JL Basset, Y Bourg, NA Broadbent, EN Brockelman, WY Bunyavejchewin, S Burslem, DFRP Butt, N Cao, M Cardenas, D Chuyong, GB Clay, K Cordell, S Dattaraja, HS Deng, XB Detto, M Du, XJ Duque, A Erikson, DL Ewango, CEN Fischer, GA Fletcher, C Foster, RB Giardina, CP Gilbert, GS Gunatilleke, N Gunatilleke, S Hao, ZQ Hargrove, WW Hart, TB Hau, BCH He, FL Hoffman, FM Howe, RW Hubbell, SP Inman-Narahari, FM Jansen, PA Jiang, MX Johnson, DJ Kanzaki, M Kassim, AR Kenfack, D Kibet, S Kinnaird, MF Korte, L Kral, K Kumar, J Larson, AJ Li, YD Li, XK Liu, SR Lum, SKY Lutz, JA Ma, KP Maddalena, DM Makana, JR Malhi, Y Marthews, T Serudin, RM McMahon, SM McShea, WJ Memiaghe, HR Mi, XC Mizuno, T Morecroft, M Myers, JA Novotny, V de Oliveira, AA Ong, PS Orwig, DA Ostertag, R den Ouden, J Parker, GG Phillips, RP Sack, L Sainge, MN Sang, WG Sri-ngernyuang, K Sukumar, R Sun, IF Sungpalee, W Suresh, HS Tan, S Thomas, SC Thomas, DW Thompson, J Turner, BL Uriarte, M Valencia, R Vallejo, MI Vicentini, A Vrska, T Wang, XH Wang, XG Weiblen, G Wolf, A Xu, H Yap, S Zimmerman, J AF Anderson-Teixeira, Kristina J. Davies, Stuart J. Bennett, Amy C. Gonzalez-Akre, Erika B. Muller-Landau, Helene C. Wright, S. Joseph Abu Salim, Kamariah Zambrano, Angelica M. Almeyda Alonso, Alfonso Baltzer, Jennifer L. Basset, Yves Bourg, Norman A. Broadbent, Eben N. Brockelman, Warren Y. Bunyavejchewin, Sarayudh Burslem, David F. R. P. Butt, Nathalie Cao, Min Cardenas, Dairon Chuyong, George B. Clay, Keith Cordell, Susan Dattaraja, Handanakere S. Deng, Xiaobao Detto, Matteo Du, Xiaojun Duque, Alvaro Erikson, David L. Ewango, Corneille E. N. Fischer, Gunter A. Fletcher, Christine Foster, Robin B. Giardina, Christian P. Gilbert, Gregory S. Gunatilleke, Nimal Gunatilleke, Savitri Hao, Zhanqing Hargrove, William W. Hart, Terese B. Hau, Billy C. H. He, Fangliang Hoffman, Forrest M. Howe, Robert W. Hubbell, Stephen P. Inman-Narahari, Faith M. Jansen, Patrick A. Jiang, Mingxi Johnson, Daniel J. Kanzaki, Mamoru Kassim, Abdul Rahman Kenfack, David Kibet, Staline Kinnaird, Margaret F. Korte, Lisa Kral, Kamil Kumar, Jitendra Larson, Andrew J. Li, Yide Li, Xiankun Liu, Shirong Lum, Shawn K. Y. Lutz, James A. Ma, Keping Maddalena, Damian M. Makana, Jean-Remy Malhi, Yadvinder Marthews, Toby Serudin, Rafizah Mat McMahon, Sean M. McShea, William J. Memiaghe, Herve R. Mi, Xiangcheng Mizuno, Takashi Morecroft, Michael Myers, Jonathan A. Novotny, Vojtech de Oliveira, Alexandre A. Ong, Perry S. Orwig, David A. Ostertag, Rebecca den Ouden, Jan Parker, Geoffrey G. Phillips, Richard P. Sack, Lawren Sainge, Moses N. Sang, Weiguo Sri-ngernyuang, Kriangsak Sukumar, Raman Sun, I-Fang Sungpalee, Witchaphart Suresh, Hebbalalu Sathyanarayana Tan, Sylvester Thomas, Sean C. Thomas, Duncan W. Thompson, Jill Turner, Benjamin L. Uriarte, Maria Valencia, Renato Vallejo, Marta I. Vicentini, Alberto Vrska, Tomas Wang, Xihua Wang, Xugao Weiblen, George Wolf, Amy Xu, Han Yap, Sandra Zimmerman, Jess TI CTFS-ForestGEO: a worldwide network monitoring forests in an era of global change SO GLOBAL CHANGE BIOLOGY LA English DT Review DE biodiversity; Center for Tropical Forest Science (CTFS); climate change; demography; forest dynamics plot; Forest Global Earth Observatory (ForestGEO); long-term monitoring; spatial analysis ID TROPICAL TREE COMMUNITY; LONG-TERM NITROGEN; RAIN-FOREST; NEOTROPICAL FOREST; FUNCTIONAL TRAITS; SPATIAL-PATTERNS; EL-NINO; PHYLOGENETIC STRUCTURE; SEEDLING RECRUITMENT; DISPERSAL LIMITATION AB Global change is impacting forests worldwide, threatening biodiversity and ecosystem services including climate regulation. Understanding how forests respond is critical to forest conservation and climate protection. This review describes an international network of 59 long-term forest dynamics research sites (CTFS-ForestGEO) useful for characterizing forest responses to global change. Within very large plots (median size 25ha), all stems 1cm diameter are identified to species, mapped, and regularly recensused according to standardized protocols. CTFS-ForestGEO spans 25 degrees S-61 degrees N latitude, is generally representative of the range of bioclimatic, edaphic, and topographic conditions experienced by forests worldwide, and is the only forest monitoring network that applies a standardized protocol to each of the world's major forest biomes. Supplementary standardized measurements at subsets of the sites provide additional information on plants, animals, and ecosystem and environmental variables. CTFS-ForestGEO sites are experiencing multifaceted anthropogenic global change pressures including warming (average 0.61 degrees C), changes in precipitation (up to +/- 30% change), atmospheric deposition of nitrogen and sulfur compounds (up to 3.8g Nm(-2)yr(-1) and 3.1g Sm(-2)yr(-1)), and forest fragmentation in the surrounding landscape (up to 88% reduced tree cover within 5km). The broad suite of measurements made at CTFS-ForestGEO sites makes it possible to investigate the complex ways in which global change is impacting forest dynamics. Ongoing research across the CTFS-ForestGEO network is yielding insights into how and why the forests are changing, and continued monitoring will provide vital contributions to understanding worldwide forest diversity and dynamics in an era of global change. C1 [Anderson-Teixeira, Kristina J.; Davies, Stuart J.; Muller-Landau, Helene C.; Wright, S. Joseph; Basset, Yves; Detto, Matteo; Gilbert, Gregory S.; Hubbell, Stephen P.; Jansen, Patrick A.; Kenfack, David; McMahon, Sean M.; Turner, Benjamin L.] Smithsonian Trop Res Inst, Ctr Trop Forest Sci, Forest Global Earth Observ, Panama City, Panama. [Anderson-Teixeira, Kristina J.; Bennett, Amy C.; Gonzalez-Akre, Erika B.; Zambrano, Angelica M. Almeyda; Bourg, Norman A.; Broadbent, Eben N.; McShea, William J.] Natl Zool Pk, Conservat Ecol Ctr, Smithsonian Conservat Biol Inst, Front Royal, VA USA. [Davies, Stuart J.; Erikson, David L.; Kenfack, David] Natl Museum Nat Hist, Dept Bot, Washington, DC 20560 USA. [Abu Salim, Kamariah] Univ Brunei Darussalam, Fac Sci, BE-1410 Bandar Seri Begawan, Brunei. [Zambrano, Angelica M. Almeyda; Broadbent, Eben N.] Stanford Univ, Stanford Woods Inst Environm, Stanford, CA 94305 USA. [Zambrano, Angelica M. Almeyda; Broadbent, Eben N.] Univ Alabama, Dept Geog, Tuscaloosa, AL USA. [Alonso, Alfonso; Korte, Lisa] Smithsonian Inst, Natl Zool Pk, Ctr Conservat Educ & Sustainabil, Smithsonian Conservat Biol Inst, Washington, DC 20008 USA. [Baltzer, Jennifer L.] Wilfrid Laurier Univ, Dept Biol, Waterloo, ON N2L 3C5, Canada. [Brockelman, Warren Y.] Mahidol Univ, Dept Biol, Bangkok 10700, Thailand. [Bunyavejchewin, Sarayudh] Dept Natl Pk Wildlife & Plant Conservat, Res Off, Bangkok, Thailand. [Burslem, David F. R. P.] Univ Aberdeen, Sch Biol Sci, Aberdeen, Scotland. [Butt, Nathalie] Univ Queensland, Sch Biol Sci, St Lucia, Qld 4072, Australia. [Butt, Nathalie; Malhi, Yadvinder; Marthews, Toby] Univ Oxford, Environm Change Inst, Sch Geog & Environm, Oxford, England. [Cao, Min; Deng, Xiaobao] Chinese Acad Sci, Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Kunming 650223, Peoples R China. [Cardenas, Dairon] Inst Amazon Invest Cient Sinchi, Bogota, Colombia. [Chuyong, George B.] Univ Buea, Dept Bot & Plant Physiol, Buea, Cameroon. [Clay, Keith; Johnson, Daniel J.; Phillips, Richard P.] Indiana Univ, Dept Biol, Bloomington, IN USA. [Cordell, Susan; Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI USA. [Dattaraja, Handanakere S.; Sukumar, Raman; Suresh, Hebbalalu Sathyanarayana] Indian Inst Sci, Ctr Ecol Sci, Bangalore 560012, Karnataka, India. [Du, Xiaojun; Ma, Keping; Mi, Xiangcheng; Sang, Weiguo] Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China. [Duque, Alvaro] Univ Nacl Colombia, Dept Ciencias Forestales, Medellin, Colombia. [Ewango, Corneille E. N.] Reserve Faune Okapis, Ctr Format & Rech Conservat Forestiere CEFRECOF E, Ituri Forest, Epulu, DEM REP CONGO. [Fischer, Gunter A.] Kadoorie Farm & Bot Garden, Tai Po, Hong Kong, Peoples R China. [Fletcher, Christine; Kassim, Abdul Rahman] Forest Res Inst Malaysia, Selangor, Malaysia. [Foster, Robin B.] Field Museum Nat Hist, Dept Bot, Chicago, IL 60605 USA. [Gilbert, Gregory S.] Univ Calif Santa Cruz, Dept Environm Studies, Santa Cruz, CA 95064 USA. [Gunatilleke, Nimal; Gunatilleke, Savitri] Univ Peradeniya, Fac Sci, Dept Bot, Peradeniya, Sri Lanka. [Hao, Zhanqing] Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110164, Peoples R China. [Hargrove, William W.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Stn Headquarters, Asheville, NC USA. [Hart, Terese B.; Wang, Xugao] Lukuru Wildlife Res Fdn, Tshuapa Lomami Lualaba Project, Kinshasa, DEM REP CONGO. [Hau, Billy C. H.] Univ Hong Kong, Kadoorie Inst, Pokfulam, Hong Kong, Peoples R China. [Hau, Billy C. H.] Univ Hong Kong, Sch Biol Sci, Pokfulam, Hong Kong, Peoples R China. [He, Fangliang] Univ Alberta, Dept Renewable Resources, Edmonton, AB, Canada. [Hoffman, Forrest M.; Kumar, Jitendra; Maddalena, Damian M.] Oak Ridge Natl Lab, Computat Earth Sci Grp, Oak Ridge, TN USA. [Howe, Robert W.] Univ Wisconsin, Dept Nat & Appl Sci, Green Bay, WI 54311 USA. [Hubbell, Stephen P.; Sack, Lawren] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA USA. [Inman-Narahari, Faith M.] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Honolulu, HI 96822 USA. [Jansen, Patrick A.] Wageningen Univ, Resource Ecol Grp, NL-6700 AP Wageningen, Netherlands. [Jiang, Mingxi] Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China. [Kanzaki, Mamoru; Mizuno, Takashi] Kyoto Univ, Grad Sch Agr, Kyoto, Japan. [Kibet, Staline] Natl Museums Kenya, Nairobi, Kenya. [Kibet, Staline] Univ Nairobi, Land Resource Management & Agr Technol Dept, Nairobi, Kenya. [Kinnaird, Margaret F.] Mpala Res Ctr, Nanyuki 10400, Kenya. [Kinnaird, Margaret F.] Wildlife Conservat Soc, Global Conservat Programs, Bronx, NY 10460 USA. [Kral, Kamil; Vrska, Tomas] Silva Tarouca Res Inst, Dept Forest Ecol, Brno, Czech Republic. [Larson, Andrew J.] Univ Montana, Coll Forestry & Conservat, Dept Forest Management, Missoula, MT 59812 USA. [Li, Yide; Xu, Han] Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou, Guangdong, Peoples R China. [Li, Xiankun] Chinese Acad Sci, Guangxi Inst Bot, Guilin, Guangxi, Peoples R China. [Liu, Shirong] Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing, Peoples R China. [Lum, Shawn K. Y.] Nanyang Technol Univ, Natl Inst Educ, Nat Sci & Sci Educ Acad Grp, Singapore 639798, Singapore. [Lutz, James A.] Utah State Univ, Wildland Resources Dept, Logan, UT 84322 USA. [Makana, Jean-Remy] Wildlife Conservat Soc, Brazzaville, DEM REP CONGO. [Serudin, Rafizah Mat] Univ Brunei Darussalam, Fac Sci, BE-1410 Bandar Seri Begawan, Brunei. [McMahon, Sean M.; Parker, Geoffrey G.] Smithsonian Environm Res Ctr, Forest Ecol Grp, Edgewater, MD 21037 USA. [Memiaghe, Herve R.] Ctr Natl Rech Sci & Technol, Inst Rech Ecol Tropicale, Libreville, Gabon. [Morecroft, Michael] Nat England, Sheffield, S Yorkshire, England. [Myers, Jonathan A.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Novotny, Vojtech] New Guinea Binatang Res Ctr, Madang, Papua N Guinea. [Novotny, Vojtech] Acad Sci Czech Republic, Ctr Biol, Ceske Budejovice 37005, Czech Republic. [Novotny, Vojtech] Univ South Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic. [de Oliveira, Alexandre A.] Univ Sao Paulo, Dept Ecol, Inst Biociencias, Sao Paulo, Brazil. [Ong, Perry S.; Yap, Sandra] Univ Philippines Diliman, Inst Biol, Quezon City, Philippines. [Orwig, David A.] Harvard Univ, Harvard Forest, Petersham, MA USA. [Ostertag, Rebecca] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. [den Ouden, Jan] Wageningen Univ, Forest Ecol & Forest Management Grp, NL-6700 AP Wageningen, Netherlands. [Sainge, Moses N.] Trop Plant Explorat Grp TroPEG, Mundemba, Southwest Regio, Cameroon. [Sun, I-Fang] Natl Dong Hwa Univ, Dept Nat Resources & Environm Studies, Hualien, Taiwan. [Tan, Sylvester] Sarawak Forest Dept, Kuching, Sarawak, Malaysia. [Thomas, Sean C.] Univ Toronto, Fac Forestry, Toronto, ON M5S 3B3, Canada. [Thomas, Duncan W.] Washington State Univ, Sch Biol Sci, Vancouver, WA USA. [Thompson, Jill] Ctr Ecol & Hydrol, Penicuik EH26 0QB, Midlothian, Scotland. [Thompson, Jill; Zimmerman, Jess] Univ Puerto Rico, Inst Trop Ecosyst Studies, Dept Environm Sci, San Juan, PR 00936 USA. [Uriarte, Maria] Columbia Univ, Dept Ecol Evolut & Environm Biol, New York, NY USA. [Valencia, Renato] Pontifical Catholic Univ Ecuador, Dept Biol Sci, Quito, Ecuador. [Vallejo, Marta I.] Inst Alexander von Humboldt, Bogota, Colombia. [Vicentini, Alberto] Inst Nacl de Pesquisas da Amazonia, Manaus, Amazonas, Brazil. [Wang, Xihua] E China Normal Univ, Sch Ecol & Environm Sci, Shanghai 200062, Peoples R China. [Weiblen, George] Univ Minnesota, Dept Plant Biol, St Paul, MN USA. [Wolf, Amy] UW Green Bay, Dept Biol, Green Bay, WI 54311 USA. [Wolf, Amy] UW Green Bay, Dept Nat & Appl Sci, Green Bay, WI 54311 USA. RP Anderson-Teixeira, KJ (reprint author), Smithsonian Trop Res Inst, Ctr Trop Forest Sci, Forest Global Earth Observ, Panama City, Panama. EM teixeirak@si.edu RI wang, xugao/B-1111-2015; Novotny, Vojtech/G-9434-2014; Basset, Yves/B-6642-2014; Giardina, Christian/C-3120-2011; Kral, Kamil/E-4415-2014; Turner, Benjamin/E-5940-2011; Thompson, Jill/K-2200-2012; Kumar, Jitendra/G-8601-2013; Wright, Stuart/M-3311-2013; Hoffman, Forrest/B-8667-2012; Sack, Lawren/A-5492-2008; Jansen, Patrick/G-2545-2015 OI Almeyda Zambrano, Angelica/0000-0001-5081-9936; Bourg, Norman/0000-0002-7443-1992; Oliveira, Alexandre/0000-0001-5526-8109; Parker, Geoffrey/0000-0001-7055-6491; Burslem, David/0000-0001-6033-0990; wang, xugao/0000-0003-1207-8852; Novotny, Vojtech/0000-0001-7918-8023; Giardina, Christian/0000-0002-3431-5073; Turner, Benjamin/0000-0002-6585-0722; Thompson, Jill/0000-0002-4370-2593; Kumar, Jitendra/0000-0002-0159-0546; Wright, Stuart/0000-0003-4260-5676; Hoffman, Forrest/0000-0001-5802-4134; Sack, Lawren/0000-0002-7009-7202; Jansen, Patrick/0000-0002-4660-0314 FU Smithsonian Competitive Grants Program for Science; Smithsonian Institution; Smithsonian Tropical Research Institute; Arnold Arboretum of Harvard University; National Science Foundation; Rockefeller Foundation; John Merck Fund; John D. and Catherine T. MacArthur Foundation; Andrew W. Mellon Foundation; Frank Levinson Family Foundation; HSBC Climate Partnership; Bromley Charitable Trust; John Swire Sons Pty Ltd; Celerity; F.H. Levinson Fund; Small World Institute Fund FX We thank everyone involved in the collection of the vast quantity of data and information in the CTFS-ForestGEO network; to F. Dentener and W. Laurance for providing data; E. Leigh, Y. Lin, J. McGarvey and A. Miller for helpful comments; E. Aikens, L. Gonzalez and M. Azimi for help with analysis and figures. Study on this manuscript was funded in part by a Smithsonian Competitive Grants Program for Science award to KJAT. The CTFS-ForestGEO network has received major support from the Smithsonian Institution - particularly the Smithsonian Tropical Research Institute, the Arnold Arboretum of Harvard University, the National Science Foundation (multiple grants), the Rockefeller Foundation, the John Merck Fund, the John D. and Catherine T. MacArthur Foundation, the Andrew W. Mellon Foundation, the Frank Levinson Family Foundation, the HSBC Climate Partnership, the Bromley Charitable Trust, John Swire & Sons Pty Ltd, Celerity, F.H. Levinson Fund, Small World Institute Fund and Jennifer and Greg Johnson. Site-specific support is listed in Table S8. NR 199 TC 60 Z9 66 U1 39 U2 205 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 528 EP 549 DI 10.1111/gcb.12712 PG 22 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400004 PM 25258024 ER PT J AU Speckman, HN Frank, JM Bradford, JB Miles, BL Massman, WJ Parton, WJ Ryan, MG AF Speckman, Heather N. Frank, John M. Bradford, John B. Miles, Brianna L. Massman, William J. Parton, William J. Ryan, Michael G. TI Forest ecosystem respiration estimated from eddy covariance and chamber measurements under high turbulence and substantial tree mortality from bark beetles SO GLOBAL CHANGE BIOLOGY LA English DT Article DE bark beetles; chambers; disturbance; EC; ecosystem respiration; respiration modeling; soil efflux; turbulence; u* filtering ID ENERGY-BALANCE CLOSURE; NET PRIMARY PRODUCTION; TROPICAL RAIN-FOREST; SOIL RESPIRATION; CARBON EXCHANGE; MAINTENANCE RESPIRATION; BLACK SPRUCE; CO2 EXCHANGE; WOODY-TISSUE; ENVIRONMENTAL CONTROLS AB Eddy covariance nighttime fluxes are uncertain due to potential measurement biases. Many studies report eddy covariance nighttime flux lower than flux from extrapolated chamber measurements, despite corrections for low turbulence. We compared eddy covariance and chamber estimates of ecosystem respiration at the GLEES Ameriflux site over seven growing seasons under high turbulence [summer night mean friction velocity (u*)=0.7ms(-1)], during which bark beetles killed or infested 85% of the aboveground respiring biomass. Chamber-based estimates of ecosystem respiration during the growth season, developed from foliage, wood, and soil CO2 efflux measurements, declined 35% after 85% of the forest basal area had been killed or impaired by bark beetles (from 7.1 +/- 0.22molm(-2)s(-1) in 2005 to 4.6 +/- 0.16molm(-2)s(-1) in 2011). Soil efflux remained at 3.3molm(-2)s(-1) throughout the mortality, while the loss of live wood and foliage and their respiration drove the decline of the chamber estimate. Eddy covariance estimates of fluxes at night remained constant over the same period, 3.0molm(-2)s(-1) for both 2005 (intact forest) and 2011 (85% basal area killed or impaired). Eddy covariance fluxes were lower than chamber estimates of ecosystem respiration (60% lower in 2005, and 32% in 2011), but the mean night estimates from the two techniques were correlated within a year (r(2) from 0.18 to 0.60). The difference between the two techniques was not the result of inadequate turbulence, because the results were robust to a u* filter of >0.7ms(-1). The decline in the average seasonal difference between the two techniques was strongly correlated with overstory leaf area (r(2)=0.92). The discrepancy between methods of respiration estimation should be resolved to have confidence in ecosystem carbon flux estimates. C1 [Speckman, Heather N.; Parton, William J.; Ryan, Michael G.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Speckman, Heather N.; Parton, William J.; Ryan, Michael G.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Speckman, Heather N.; Frank, John M.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Speckman, Heather N.; Frank, John M.] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Frank, John M.; Massman, William J.; Ryan, Michael G.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Bradford, John B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Miles, Brianna L.] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA. RP Speckman, HN (reprint author), Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. EM hspeckma@uwyo.edu RI Bradford, John/E-5545-2011; Ryan, Michael/A-9805-2008 OI Ryan, Michael/0000-0002-2500-6738 FU US Forest Service Rocky Mountain Research Station through the Forest Service National Climate Change Program; NASA [CARBON/04-0225-0191]; CSIRO's McMaster Fellowship FX We thank Raechel Owens, Erik Skeie, Chase Jones, and Lance Asherin for data collection and Ben Bird, Kristen Scott, and Kurt Speckman for assistance with data analysis and prose. This research was funded by US Forest Service Rocky Mountain Research Station through the Forest Service National Climate Change Program. Plot data collection, including soil respiration prior to 2010 was funded by NASA grants CARBON/04-0225-0191. Further thanks to A. Scott Denning and Jay Ham MG Ryan acknowledges the support of CSIRO's McMaster Fellowship during manuscript preparation. NR 91 TC 12 Z9 12 U1 6 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 708 EP 721 DI 10.1111/gcb.12731 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400018 PM 25205425 ER PT J AU Abella, SR Fornwalt, PJ AF Abella, Scott R. Fornwalt, Paula J. TI Ten years of vegetation assembly after a North American mega fire SO GLOBAL CHANGE BIOLOGY LA English DT Article DE disturbance; exotic species; fire severity; Hayman Fire; Pinus ponderosa; resilience; resistance; succession; vegetation change ID PONDEROSA PINE FORESTS; MIXED-SEVERITY WILDFIRE; COLORADO-FRONT-RANGE; SUCCESSIONAL PATHWAYS; MANAGEMENT TREATMENTS; RESILIENCE THINKING; LANDSCAPE PATTERNS; PLANT-COMMUNITIES; NATIONAL-PARK; ARIZONA AB Altered fuels and climate change are transforming fire regimes in many of Earth's biomes. Postfire reassembly of vegetation - paramount to C storage and biodiversity conservation - frequently remains unpredictable and complicated by rapid global change. Using a unique data set of pre and long-term postfire data, combined with long-term data from nearby unburned areas, we examined 10years of understory vegetation assembly after the 2002 Hayman Fire. This fire was the largest wildfire in recorded history in Colorado, USA. Resistance (initial postfire deviance from prefire condition) and resilience (return to prefire condition) declined with increasing fire severity. However, via both resistance and resilience, legacy' species of the prefire community constituted >75% of total plant cover within 3years even in severely burned areas. Perseverance of legacy species, coupled with new colonizers, created a persistent increase in community species richness and cover over prefire levels. This was driven by a first-year increase (maintained over time) in forbs with short life spans; a 2-3-year delayed surge in long-lived forbs; and a consistent increase in graminoids through the 10th postfire year. Burning increased exotic plant invasion relative to prefire and unburned areas, but burned communities always were >89% native. This study informs debate in the literature regarding whether these increasingly large fires are ecological catastrophes.' Landscape-scale severe burning was catastrophic from a tree overstory perspective, but from an understory perspective, burning promoted rich and productive native understories, despite the entire 10-year postfire period receiving below-average precipitation. C1 [Abella, Scott R.] Natl Pk Serv, Biol Resource Management Div, Washington Off, Nat Resource Stewardship & Sci Directorate, Ft Collins, CO 80525 USA. [Fornwalt, Paula J.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Abella, SR (reprint author), Nat Resource Conservat LLC, 1400 Colorado St, Boulder City, NV 89005 USA. EM abellanrc@gmail.com FU Joint Fire Science Program [03-2-3-08, 04-2-1-118, 11-1-1-5]; U.S. Forest Service, Rocky Mountain Research Station via National Park Service FX This study was funded by the Joint Fire Science Program (project numbers 03-2-3-08, 04-2-1-118, and 11-1-1-5) and the U.S. Forest Service, Rocky Mountain Research Station, partly via interagency agreement with the National Park Service. We thank Stephanie Asherin, Marin Chambers, Allison Grow, Rebecca Hemmerling, Micky McNaughton, Jill Oropeza, Lisa Schell, Rick Shory, Betsy Smith, and Jennifer Ventker for assisting with fieldwork and data management; Scott Baggett for statistical advice; Sharon Altman for formatting figures; and Subject Editor B.E. Law, two anonymous reviewers, Pete Budde, and Sharon Altman for reviewing the manuscript. Lodging, laboratory, and office facilities during fieldwork were provided by the Manitou Experimental Forest of the U.S. Forest Service. This article was prepared by U.S. Government employees on official time and is in the public domain in the USA. NR 62 TC 12 Z9 12 U1 13 U2 51 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 789 EP 802 DI 10.1111/gcb.12722 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400024 PM 25200376 ER PT J AU Novick, KA Oishi, AC Ward, EJ Siqueira, MBS Juang, JY Stoy, PC AF Novick, Kimberly A. Oishi, A. Christopher Ward, Eric J. Siqueira, Mario B. S. Juang, Jehn-Yih Stoy, Paul C. TI On the difference in the net ecosystem exchange of CO2 between deciduous and evergreen forests in the southeastern United States SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon flux; drought; eddy covariance; evapotranspiration; net ecosystem exchange; water use efficiency; wavelet spectra ID ELEVATED ATMOSPHERIC CO2; SAP FLUX MEASUREMENTS; WATER-VAPOR EXCHANGE; LAND-USE CHANGE; CARBON-DIOXIDE; PINE FOREST; STOMATAL CONDUCTANCE; HARDWOOD FORESTS; WAVELET ANALYSIS; NORTH FLORIDA AB The southeastern United States is experiencing a rapid regional increase in the ratio of pine to deciduous forest ecosystems at the same time it is experiencing changes in climate. This study is focused on exploring how these shifts will affect the carbon sink capacity of southeastern US forests, which we show here are among the strongest carbon sinks in the continental United States. Using eight-year-long eddy covariance records collected above a hardwood deciduous forest (HW) and a pine plantation (PP) co-located in North Carolina, USA, we show that the net ecosystem exchange of CO2 (NEE) was more variable in PP, contributing to variability in the difference in NEE between the two sites (NEE) at a range of timescales, including the interannual timescale. Because the variability in evapotranspiration (ET) was nearly identical across the two sites over a range of timescales, the factors that determined the variability in NEE were dominated by those that tend to decouple NEE from ET. One such factor was water use efficiency, which changed dramatically in response to drought and also tended to increase monotonically in nondrought years (P<0.001 in PP). Factors that vary over seasonal timescales were strong determinants of the NEE in the HW site; however, seasonality was less important in the PP site, where significant amounts of carbon were assimilated outside of the active season, representing an important advantage of evergreen trees in warm, temperate climates. Additional variability in the fluxes at long-time scales may be attributable to slowly evolving factors, including canopy structure and increases in dormant season air temperature. Taken together, study results suggest that the carbon sink in the southeastern United States may become more variable in the future, owing to a predicted increase in drought frequency and an increase in the fractional cover of southern pines. C1 [Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. [Novick, Kimberly A.; Oishi, A. Christopher; Ward, Eric J.; Siqueira, Mario B. S.; Juang, Jehn-Yih; Stoy, Paul C.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Oishi, A. Christopher] US Forest Serv, Coweeta Hydrol Lab, Southern Res Stn, USDA, Otto, NC 28763 USA. [Ward, Eric J.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Siqueira, Mario B. S.] Univ Brasilia, Dept Mech Engn, Brasilia, DF, Brazil. [Juang, Jehn-Yih] Natl Taiwan Univ, Dept Geog, Taipei 10617, Taiwan. [Stoy, Paul C.] Montana State Univ, Dept Land Resources & Environm Studies, Bozeman, MT 59717 USA. RP Novick, KA (reprint author), Indiana Univ, Sch Publ & Environm Affairs, 702 N Walnut Grove Ave, Bloomington, IN 47405 USA. EM knovick@indiana.edu RI Ward, Eric/D-7131-2017; OI Ward, Eric/0000-0002-5047-5464; Juang, Jehn-Yih/0000-0002-7890-0696 FU Office of Science (BER), US Department of Energy [FG02-95SER62083]; NSF Graduate Research Fellowship Program [DGE1106401]; James B. Duke Fellowship Program FX This research was sponsored by the Office of Science (BER), US Department of Energy (FG02-95SER62083), by the NSF Graduate Research Fellowship Program (DGE1106401), and the James B. Duke Fellowship Program. We thank Gaby Katul and Ram Oren for their long-term oversight of the Duke Forest flux towers, and for helpful feedback on an earlier version of this manuscript. The authors confirm that they have no interest or relationship, financial, or otherwise that might be perceived as influencing objectivity with respect to the work described in this study. NR 78 TC 6 Z9 6 U1 4 U2 55 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 827 EP 842 DI 10.1111/gcb.12723 PG 16 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400027 PM 25168968 ER PT J AU Gustafson, EJ De Bruijn, AMG Pangle, RE Limousin, JM McDowell, NG Pockman, WT Sturtevant, BR Muss, JD Kubiske, ME AF Gustafson, Eric J. De Bruijn, Arjan M. G. Pangle, Robert E. Limousin, Jean-Marc McDowell, Nate G. Pockman, William T. Sturtevant, Brian R. Muss, Jordan D. Kubiske, Mark E. TI Integrating ecophysiology and forest landscape models to improve projections of drought effects under climate change SO GLOBAL CHANGE BIOLOGY LA English DT Article DE climate change; competition for light; drought; forest landscape disturbance and succession model; LANDIS-II; Pinon-juniper ecosystem; PnET-Succession; tree mortality; water stress ID PINYON-JUNIPER WOODLAND; VEGETATION MORTALITY; SIMULATION-MODEL; ATMOSPHERIC CO2; TREE MORTALITY; UNITED-STATES; LANDIS-II; PLANTS; MECHANISMS; DYNAMICS AB Fundamental drivers of ecosystem processes such as temperature and precipitation are rapidly changing and creating novel environmental conditions. Forest landscape models (FLM) are used by managers and policy-makers to make projections of future ecosystem dynamics under alternative management or policy options, but the links between the fundamental drivers and projected responses are weak and indirect, limiting their reliability for projecting the impacts of climate change. We developed and tested a relatively mechanistic method to simulate the effects of changing precipitation on species competition within the LANDIS-II FLM. Using data from a field precipitation manipulation experiment in a pinon pine (Pinus edulis) and juniper (Juniperus monosperma) ecosystem in New Mexico (USA), we calibrated our model to measurements from ambient control plots and tested predictions under the drought and irrigation treatments against empirical measurements. The model successfully predicted behavior of physiological variables under the treatments. Discrepancies between model output and empirical data occurred when the monthly time step of the model failed to capture the short-term dynamics of the ecosystem as recorded by instantaneous field measurements. We applied the model to heuristically assess the effect of alternative climate scenarios on the pinon-juniper ecosystem and found that warmer and drier climate reduced productivity and increased the risk of drought-induced mortality, especially for pinon. We concluded that the direct links between fundamental drivers and growth rates in our model hold great promise to improve our understanding of ecosystem processes under climate change and improve management decisions because of its greater reliance on first principles. C1 [Gustafson, Eric J.; De Bruijn, Arjan M. G.; Sturtevant, Brian R.; Kubiske, Mark E.] US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, USDA, Rhinelander, WI 54501 USA. [De Bruijn, Arjan M. G.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Pangle, Robert E.; Limousin, Jean-Marc; Pockman, William T.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [McDowell, Nate G.; Muss, Jordan D.] Los Alamos Natl Lab, Earth & Environm Sci Div, Los Alamos, NM 87545 USA. RP Gustafson, EJ (reprint author), US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, USDA, 5985 Highway K, Rhinelander, WI 54501 USA. EM egustafson@fs.fed.us RI Pockman, William/D-4086-2014 OI Pockman, William/0000-0002-3286-0457 FU Northern Research Station of the USDA Forest Service; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [105321]; US Department of Energy (BER); National Science Foundation via the Sevilleta LTER program FX Funding was provided by the Northern Research Station of the USDA Forest Service and an Agriculture and Food Research Initiative Competitive Grant (no. 105321) to Purdue University from the USDA National Institute of Food and Agriculture. The Sevilleta drought experiment was funded by the US Department of Energy (BER) with additional support from the National Science Foundation via the Sevilleta LTER program. Thanks to Rich Birdsey, Neil Cobb, and anonymous reviewers for critical reviews of the manuscript. NR 50 TC 7 Z9 7 U1 8 U2 59 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 843 EP 856 DI 10.1111/gcb.12713 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400028 PM 25155807 ER PT J AU Martre, P Wallach, D Asseng, S Ewert, F Jones, JW Rotter, RP Boote, KJ Ruane, AC Thorburn, PJ Cammarano, D Hatfield, JL Rosenzweig, C Aggarwal, PK Angulo, C Basso, B Bertuzzi, P Biernath, C Brisson, N Challinor, AJ Doltra, J Gayler, S Goldberg, R Grant, RF Heng, L Hooker, J Hunt, LA Ingwersen, J Izaurralde, RC Kersebaum, KC Muller, C Kumar, SN Nendel, C O'leary, G Olesen, JE Osborne, TM Palosuo, T Priesack, E Ripoche, D Semenov, MA Shcherbak, I Steduto, P Stockle, CO Stratonovitch, P Streck, T Supit, I Tao, FL Travasso, M Waha, K White, JW Wolf, J AF Martre, Pierre Wallach, Daniel Asseng, Senthold Ewert, Frank Jones, James W. Rotter, Reimund P. Boote, Kenneth J. Ruane, Alex C. Thorburn, Peter J. Cammarano, Davide Hatfield, Jerry L. Rosenzweig, Cynthia Aggarwal, Pramod K. Angulo, Carlos Basso, Bruno Bertuzzi, Patrick Biernath, Christian Brisson, Nadine Challinor, Andrew J. Doltra, Jordi Gayler, Sebastian Goldberg, Richie Grant, Robert F. Heng, Lee Hooker, Josh Hunt, Leslie A. Ingwersen, Joachim Izaurralde, Roberto C. Kersebaum, Kurt Christian Mueller, Christoph Kumar, Soora Naresh Nendel, Claas O'leary, Garry Olesen, Jorgen E. Osborne, Tom M. Palosuo, Taru Priesack, Eckart Ripoche, Dominique Semenov, Mikhail A. Shcherbak, Iurii Steduto, Pasquale Stoeckle, Claudio O. Stratonovitch, Pierre Streck, Thilo Supit, Iwan Tao, Fulu Travasso, Maria Waha, Katharina White, Jeffrey W. Wolf, Joost TI Multimodel ensembles of wheat growth: many models are better than one SO GLOBAL CHANGE BIOLOGY LA English DT Article DE ecophysiological model; ensemble modeling; model intercomparison; process-based model; uncertainty; wheat (Triticum aestivum L.) ID CLIMATE-CHANGE; CROP PRODUCTION; IMPACTS; YIELD; SIMULATIONS; CALIBRATION; AUSTRALIA; BILLION; EUROPE; GRAIN AB Crop models of crop growth are increasingly used to quantify the impact of global changes due to climate or crop management. Therefore, accuracy of simulation results is a major concern. Studies with ensembles of crop models can give valuable information about model accuracy and uncertainty, but such studies are difficult to organize and have only recently begun. We report on the largest ensemble study to date, of 27 wheat models tested in four contrasting locations for their accuracy in simulating multiple crop growth and yield variables. The relative error averaged over models was 24-38% for the different end-of-season variables including grain yield (GY) and grain protein concentration (GPC). There was little relation between error of a model for GY or GPC and error for in-season variables. Thus, most models did not arrive at accurate simulations of GY and GPC by accurately simulating preceding growth dynamics. Ensemble simulations, taking either the mean (e-mean) or median (e-median) of simulated values, gave better estimates than any individual model when all variables were considered. Compared to individual models, e-median ranked first in simulating measured GY and third in GPC. The error of e-mean and e-median declined with an increasing number of ensemble members, with little decrease beyond 10 models. We conclude that multimodel ensembles can be used to create new estimators with improved accuracy and consistency in simulating growth dynamics. We argue that these results are applicable to other crop species, and hypothesize that they apply more generally to ecological system models. C1 [Martre, Pierre] INRA, Genet Divers & Ecophysiol Cereals GDEC UMR1095, 5 Chemin Beaulieu, F-63100 Clermont Ferrand, France. [Martre, Pierre] Univ Blaise Pascal, GDEC UMR1095, F-63170 Aubiere, France. [Wallach, Daniel] INRA, Agrosyst & Dev Terr UMR1248, F-31326 Castanet Tolosan, France. [Asseng, Senthold; Jones, James W.; Boote, Kenneth J.; Cammarano, Davide] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. [Ewert, Frank; Angulo, Carlos] Univ Bonn, Inst Crop Sci & Resource Conservat, D-53115 Bonn, Germany. [Rotter, Reimund P.; Palosuo, Taru] MTT Agrifood Res Finland, Plant Prod Res, FI-50100 Mikkeli, Finland. [Ruane, Alex C.; Rosenzweig, Cynthia; Goldberg, Richie] NASA, Goddard Inst Space Studies, New York, NY 10025 USA. [Thorburn, Peter J.] Commonwealth Sci & Ind Res Org, Ecosyst Sci, Dutton Pk, Qld 4102, Australia. [Hatfield, Jerry L.] Natl Lab Agr & Environm, Ames, IA 50011 USA. [Aggarwal, Pramod K.] Int Water Management Inst, Consultat Grp Int Agr Res, Res Program Climate Change Agr & Food Secur, New Delhi 110012, India. [Basso, Bruno; Shcherbak, Iurii] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48823 USA. [Basso, Bruno; Shcherbak, Iurii] Michigan State Univ, Kellogg Biol Stn, E Lansing, MI 48823 USA. [Bertuzzi, Patrick; Ripoche, Dominique] INRA, AgroClim US1116, F-84914 Avignon, France. [Biernath, Christian; Priesack, Eckart] Helmholtz Zentrum Munchen, Inst Soil Ecol, German Res Ctr Environm Hlth, D-85764 Neuherberg, Germany. [Brisson, Nadine] INRA, Agron UMR0211, F-78750 Thiverval Grignon, France. [Brisson, Nadine] AgroParisTech, Agron UMR0211, F-78750 Thiverval Grignon, France. [Challinor, Andrew J.] Univ Leeds, Sch Earth & Environm, Inst Climate & Atmospher Sci, Leeds LS2 9JT, W Yorkshire, England. [Challinor, Andrew J.] Ctr Int Agr Trop, CGIAR ESSP Program Climate Change Agr & Food Secu, Cali 6713, Colombia. [Doltra, Jordi] Cantabrian Agr Res & Training Ctr, Muriedas 39600, Spain. [Gayler, Sebastian] Univ Tubingen, Water & Earth Syst Sci Competence Cluster, D-72074 Tubingen, Germany. [Grant, Robert F.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada. [Heng, Lee] IAEA, A-1400 Vienna, Austria. [Hooker, Josh] Univ Reading, Sch Agr Policy & Dev, Reading RG6 6AR, Berks, England. [Hunt, Leslie A.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Ingwersen, Joachim; Streck, Thilo] Univ Stuttgart Hohenheim, Inst Soil Sci & Land Evaluat, D-70599 Stuttgart, Germany. [Izaurralde, Roberto C.] Univ Maryland, Dept Geog Sci, Hyattsville, MD 20782 USA. [Kersebaum, Kurt Christian; Nendel, Claas] Leibniz Ctr Agr Landscape Res, Inst Landscape Syst Anal, D-15374 Muncheberg, Germany. [Mueller, Christoph; Waha, Katharina] Potsdam Inst Climate Impact Res, D-14473 Potsdam, Germany. [Kumar, Soora Naresh] Indian Agr Res Inst, Ctr Environm Sci & Climate Resilient Agr, New Delhi 110012, India. [O'leary, Garry] Dept Primary Ind Landscape & Water Sci, Horsham, Vic 3400, Australia. [Olesen, Jorgen E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Osborne, Tom M.] Univ Reading, Dept Meteorol, Natl Ctr Atmospher Sci, Reading RG6 6BB, Berks, England. [Semenov, Mikhail A.; Stratonovitch, Pierre] Rothamsted Res, Computat & Syst Biol Dept, Harpenden AL5 2JQ, Herts, England. [Steduto, Pasquale] Food & Agr Org United Nations, I-00153 Rome, Italy. [Stoeckle, Claudio O.] Washington State Univ, Pullman, WA 99164 USA. [Supit, Iwan; Wolf, Joost] Wageningen Univ, NL-6700 AA Wageningen, Netherlands. [Tao, Fulu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Travasso, Maria] INTA CIRN, Inst Climate & Water, RA-1712 Castelar, Argentina. [White, Jeffrey W.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Martre, P (reprint author), INRA, Genet Divers & Ecophysiol Cereals GDEC UMR1095, 5 Chemin Beaulieu, F-63100 Clermont Ferrand, France. EM pierre.martre@clermont.inra.fr RI Challinor, Andrew/C-4992-2008; Palosuo, Taru/B-9593-2012; Doltra, Jordi/C-2106-2015; Thorburn, Peter/A-6884-2011; Priesack, Eckart/M-7341-2014; Martre, Pierre/G-5399-2013; Mueller, Christoph/E-4812-2016; Olesen, Jorgen/C-2905-2016 OI Cammarano, Davide/0000-0003-0918-550X; Wallach, Daniel/0000-0003-3500-8179; Priesack, Eckart/0000-0002-5088-9528; Grant, Robert/0000-0002-8890-6231; Boote, Kenneth/0000-0002-1358-5496; Kersebaum, Kurt Christian/0000-0002-3679-8427; Stratonovitch, Pierre/0000-0002-5806-2066; Challinor, Andrew/0000-0002-8551-6617; Palosuo, Taru/0000-0003-4322-3450; Martre, Pierre/0000-0002-7419-6558; Mueller, Christoph/0000-0002-9491-3550; Olesen, Jorgen/0000-0002-6639-1273 NR 45 TC 43 Z9 43 U1 12 U2 101 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 911 EP 925 DI 10.1111/gcb.12768 PG 15 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400033 PM 25330243 ER PT J AU Bansal, S Harrington, CA Gould, PJ St Clair, JB AF Bansal, Sheel Harrington, Constance A. Gould, Peter J. St Clair, J. Bradley TI Climate-related genetic variation in drought-resistance of Douglas-fir (Pseudotsuga menziesii) SO GLOBAL CHANGE BIOLOGY LA English DT Article DE climate change; genecology; minimum conductance; minimum transpiration; specific leaf area; water deficit ID CUTICULAR WATER PERMEABILITY; ALEPPO PINE-SEEDLINGS; PICEA-ABIES; TREE IMPROVEMENT; PLANT-RESPONSES; SEED TRANSFER; MIRB. FRANCO; OREGON; TRANSPIRATION; COASTAL AB There is a general assumption that intraspecific populations originating from relatively arid climates will be better adapted to cope with the expected increase in drought from climate change. For ecologically and economically important species, more comprehensive, genecological studies that utilize large distributions of populations and direct measures of traits associated with drought-resistance are needed to empirically support this assumption because of the implications for the natural or assisted regeneration of species. We conducted a space-for-time substitution, common garden experiment with 35 populations of coast Douglas-fir (Pseudotsuga menziesii var. menziesii) growing at three test sites with distinct summer temperature and precipitation (referred to as cool/moist', moderate', or warm/dry') to test the hypotheses that (i) there is large genetic variation among populations and regions in traits associated with drought-resistance, (ii) the patterns of genetic variation are related to the native source-climate of each population, in particular with summer temperature and precipitation, (iii) the differences among populations and relationships with climate are stronger at the warm/dry test site owing to greater expression of drought-resistance traits (i.e., a genotypexenvironment interaction). During midsummer 2012, we measured the rate of water loss after stomatal closure (transpiration(min)), water deficit (% below turgid saturation), and specific leaf area (SLA, cm(2)g(-1)) on new growth of sapling branches. There was significant genetic variation in all plant traits, with populations originating from warmer and drier climates having greater drought-resistance (i.e., lower transpiration(min), water deficit and SLA), but these trends were most clearly expressed only at the warm/dry test site. Contrary to expectations, populations from cooler climates also had greater drought-resistance across all test sites. Multiple regression analysis indicated that Douglas-fir populations from regions with relatively cool winters and arid summers may be most adapted to cope with drought conditions that are expected in the future. C1 [Bansal, Sheel; Harrington, Constance A.; Gould, Peter J.] US Forest Serv, Pacific NW Res Stn, USDA, Olympia, WA 98512 USA. [Gould, Peter J.] Washington State Dept Nat Resources, Olympia, WA 98504 USA. [St Clair, J. Bradley] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP Bansal, S (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM sheelbansal9@gmail.com OI Bansal, Sheel/0000-0003-1233-1707 NR 68 TC 15 Z9 15 U1 6 U2 70 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD FEB PY 2015 VL 21 IS 2 BP 947 EP 958 DI 10.1111/gcb.12719 PG 12 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA CA1DE UT WOS:000348652400036 PM 25156589 ER PT J AU Gao, S Wang, XP Wang, LH AF Gao, Shan Wang, Xiping Wang, Lihai TI Modeling temperature effect on dynamic modulus of elasticity of red pine (Pinus resinosa) in frozen and non-frozen states SO HOLZFORSCHUNG LA English DT Article DE freezing point; ice formation; modulus of elasticity; moisture content; ultrasonic velocity ID FIBER-SATURATION POINT; MECHANICAL-PROPERTIES; MOISTURE-CONTENT; STANDING TREES; ACOUSTIC EVALUATION; BENDING PROPERTIES; WOOD; LUMBER AB The response of dynamic and static modulus of elasticity (MOEdyn and MOEsta) of red pine small clear wood (25.4x25.4x407 mm(3)) within the temperature range -40 to 40 degrees C has been investigated. The moisture content (MC) of the specimens ranged from 0 to 118%. The MOEdyn was calculated based on measured ultrasonic velocity (V) and wood density. The MOEsta was measured by static bending tests in a climate chamber between -40 and 40 degrees C. The results indicate that both MOEdyn and MOEsta were affected by temperature and the MC. Above freezing point, MOE decreased linearly at a slow rate with increasing temperature. Below freezing point, MOE increased at a rapid rate with decreasing temperature. The MC-level had a significant effect on the MOE-temperature relationships. Temperature effect was much more significant in green wood than in dry wood. Analytical models were developed to predict the change of MOEdyn relative to that at 20 degrees C in the case of acoustic measurements under different temperature conditions. C1 [Gao, Shan; Wang, Lihai] Northeast Forestry Univ, Coll Engn & Technol, Harbin 150040, Peoples R China. [Wang, Xiping] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. RP Gao, S (reprint author), Northeast Forestry Univ, Coll Engn & Technol, Harbin 150040, Peoples R China. EM sgao.nefu@gmail.com FU China Fundamental Research Funds for the Central Universities [DL12BB31] FX This project was conducted under the cooperative research agreement between Northeast Forestry University, Harbin, China and the USDA Forest Service, Forest Products Laboratory (FPL), Madison, WI, USA, and was supported in part by the China Fundamental Research Funds for the Central Universities (Grant No. DL12BB31). We thank Mr. James T. Gilbertson of FPL for his technical assistance during the laboratory experiments. We also thank Dr. Robert Ross of FPL and Mr. Brain Brashaw of University of Minnesota, Duluth, USA, for their support to this project. NR 16 TC 3 Z9 3 U1 1 U2 9 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD FEB PY 2015 VL 69 IS 2 BP 233 EP 240 DI 10.1515/hf-2014-0048 PG 8 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA CA4FD UT WOS:000348858800014 ER PT J AU Pinto, VZ Moomand, K Vanier, NL Colussi, R Villanova, FA Zavareze, ER Lim, LT Dias, ARG AF Pinto, Vania Z. Moomand, Khalid Vanier, Nathan L. Colussi, Rosana Villanova, Franciene A. Zavareze, Elessandra R. Lim, Loong-Tak Dias, Alvaro R. G. TI Molecular structure and granule morphology of native and heat-moisture-treated pinhao starch SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Carbohydrates; FTIR; heat-moisture treatment; pinhAo starch ID TRANSFORM INFRARED-SPECTROSCOPY; WAXY POTATO STARCHES; PHYSICOCHEMICAL PROPERTIES; THERMAL-PROPERTIES; PHYSICAL-CHARACTERISTICS; CRYSTALLINE-STRUCTURE; AMYLOSE CONTENT; ROOT STARCHES; RICE STARCH; AMYLOPECTIN AB PinhAo seed is an unconventional source of starch and the pines grow up in native forests of southern Latin America. In this study, pinhAo starch was adjusted at 15, 20 and 25% moisture content and heated to 100, 110 and 120 degrees C for 1h. A decrease in max (starch/iodine complex) was observed as a result of increase in temperature and moisture content of HMT. The ratio of crystalline to amorphous phase in pinhAo starch was determined via Fourier transform infra red by taking 1045/1022 band ratio. A decrease in crystallinity occurred as a result of HMT. Polarised light microscopy indicated a loss of birefringence of starch granules under 120 degrees C at 25% moisture content. Granule size distribution was further confirmed via scanning electron microscopy which showed the HMT effects. These results increased the understanding on molecular and structural properties of HMT pinhAo starch and broadened its food and nonfood industrial applications. C1 [Pinto, Vania Z.; Vanier, Nathan L.; Colussi, Rosana; Villanova, Franciene A.; Zavareze, Elessandra R.; Dias, Alvaro R. G.] Univ Fed Pelotas, Dept Ciencia & Tecnol Agroind, BR-96010900 Pelotas, RS, Brazil. [Pinto, Vania Z.; Moomand, Khalid; Lim, Loong-Tak] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada. [Vanier, Nathan L.] ARS, Hlth Proc Foods Res Unit, WRRC, USDA, Albany, CA 94710 USA. RP Pinto, VZ (reprint author), Univ Fed Pelotas, Dept Ciencia & Tecnol Agroind, BR-96010900 Pelotas, RS, Brazil. EM vania_vzp@hotmail.com OI Vanier, Nathan/0000-0001-6592-3023; Zanella Pinto, Vania/0000-0002-7081-5446 FU CAPES; FAPERGS; CNPq; SCT-RS; Polo de Inovacao Tecnologica em Alimentos da Regiao Sul FX We would like to thank CAPES, FAPERGS, CNPq and also to SCT-RS and Polo de Inovacao Tecnologica em Alimentos da Regiao Sul for financial support. We are also very grateful towards Drs. Milena Corredig and Koushik Seetharaman at the Department of Food Science, University of Guelph, for allowing us to utilise their laboratory facilities to complete some of the analyses and also to Dr. Sandy Smith for her assistance in microscopy image acquisitions. NR 49 TC 4 Z9 4 U1 8 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD FEB PY 2015 VL 50 IS 2 BP 282 EP 289 DI 10.1111/ijfs.12608 PG 8 WC Food Science & Technology SC Food Science & Technology GA CA0BE UT WOS:000348578600002 ER PT J AU Chen, CYO Kamil, A Blumberg, JB AF Chen, C-Y. Oliver Kamil, Alison Blumberg, Jeffrey B. TI Phytochemical composition and antioxidant capacity of whole wheat products SO INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION LA English DT Article DE Alkylresorcinols; phenolics; phytosterosl; tocols; total antioxidant capacity; whole wheat ID HEALTHGRAIN DIVERSITY SCREEN; CEREAL PRODUCTS; BIOACTIVE COMPOUNDS; QUINONE REDUCTASE; PHENOLIC-ACIDS; ALKYLRESORCINOLS; GRAINS; RYE; TOCOTRIENOLS; TOCOPHEROLS AB Whole wheat contains an array of phytochemicals. We quantified alkylresorcinols (AR), phenolic acids, phytosterols, and tocols in six whole wheat products and characterized their antioxidant capacity and ability to induce quinone reductase activity (QR). Total AR content ranged from 136.8 to 233.9 mu g/g and was correlated with whole wheat content (r = 0.9248; p = 0.0083). Ferulic acid (FerA) was the dominant phenolic at 99.9-316.0 mu g/g and mostly bound tightly to the wheat matrix. AR-C21 and total FerA predicted the whole wheat content in each product (R-2 = 0.9933). Total phytosterol content ranged from 562.6 to 1035.5 mu g/g. Total tocol content ranged from 19.3 to 292.7 mu g/g. Phytosterol and tocol contents were independent of whole wheat content. Whole wheat biscuits and pasta were the most potent products to induce QR in Hepa1c1c7 cells. This study provides a platform to characterize the relationship between the phytochemical composition of whole wheat and products formulated with this whole grain. C1 [Chen, C-Y. Oliver; Kamil, Alison; Blumberg, Jeffrey B.] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Chen, CYO (reprint author), Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM oliver.chen@tufts.edu FU Kraft Foods Group Inc.; U.S. Department of Agriculture (USDA)/Agricultural Research Service [58-1950-0-014] FX This study was supported by Kraft Foods Group Inc. and U.S. Department of Agriculture (USDA)/Agricultural Research Service under Cooperative Agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 29 TC 1 Z9 1 U1 6 U2 41 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 0963-7486 EI 1465-3478 J9 INT J FOOD SCI NUTR JI Int. J. Food Sci. Nutr. PD FEB PY 2015 VL 66 IS 1 BP 63 EP 70 DI 10.3109/09637486.2014.971228 PG 8 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CA9EJ UT WOS:000349223200005 PM 25578763 ER PT J AU Wells, JE Berry, ED Guerini, MN Varel, VH AF Wells, J. E. Berry, E. D. Guerini, M. N. Varel, V. H. TI Evaluation of essential oils in beef cattle manure slurries and applications of select compounds to beef feedlot surfaces to control zoonotic pathogens SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE animal waste; antimicrobial agents; cattle; feedlot manure; odour; pathogens ID ESCHERICHIA-COLI O157-H7; CAMPYLOBACTER-JEJUNI; UREASE INHIBITOR; LISTERIA-MONOCYTOGENES; MULTIPLEX PCR; SOIL-MOISTURE; BOVINE FECES; SWINE WASTE; ODOR; BACTERIA AB AimsTo evaluate natural terpene compounds for antimicrobial activities and determine whether these compounds could be used to control microbial activities and pathogens in production animal facilities. Methods and ResultsThymol, geraniol, glydox, linalool, pine oil, plinol and terpineol were tested in laboratory studies for ability to control the production of odorous volatile fatty acid compounds and reduce pathogen levels in manure slurry preparations. Thymol is a terpene phenolic compound and was most effective for reducing fermentation products and pathogen levels (P<005), followed by the extracts linalool, pine oil and terpineol, which are terpene alcohols. Select compounds thymol, linalool and pine oil were further evaluated in two separate studies by applying the agents to feedlot surfaces in cattle pens. Feedlot surface material (FSM; manure and soil) was collected and analysed for fermentation products, levels of coliforms and total Escherichia coli, and the presence of E.coli O157:H7, Campylobacter, Salmonella, Listeria and L.monocytogenes. The reduction in fermentation products but not pathogens was dependent on the moisture present in the FSM. Treatment with 2000ppm thymol reduced the prevalence of E.coli O157:H7 but not Listeria. In a separate study, treatment with 4000ppm pine oil reduced E.coli O157:H7, Listeria and Campylobacter (P<005). Linalool was tested at two levels (2000 and 4000ppm) and did not affect pathogen levels at either concentration. ConclusionsNatural compounds bearing terpenes can control pathogenic bacteria in treated manures and when applied to the feedlot surface in production cattle systems. Pine oil is a cheaper alternative to thymol and may be a useful treatment for controlling pathogens. Significance and Impact of the StudyThe control of bacterial pathogens in animal productions systems is an important step in preharvest food safety. Waste products, such as pine oil extract, from the pulp wood industry may have application for treating feedlot pens and manures to reduce the pathogen load. C1 [Wells, J. E.; Berry, E. D.; Guerini, M. N.; Varel, V. H.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Wells, JE (reprint author), USDA ARS, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM jim.wells@ars.usda.gov NR 32 TC 2 Z9 2 U1 3 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD FEB PY 2015 VL 118 IS 2 BP 295 EP 304 DI 10.1111/jam.12689 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA4DM UT WOS:000348854500004 PM 25385364 ER PT J AU Beier, RC Foley, SL Davidson, MK White, DG McDermott, PF Bodeis-Jones, S Zhao, S Andrews, K Crippen, TL Sheffield, CL Poole, TL Anderson, RC Nisbet, DJ AF Beier, R. C. Foley, S. L. Davidson, M. K. White, D. G. McDermott, P. F. Bodeis-Jones, S. Zhao, S. Andrews, K. Crippen, T. L. Sheffield, C. L. Poole, T. L. Anderson, R. C. Nisbet, D. J. TI Characterization of antibiotic and disinfectant susceptibility profiles among Pseudomonas aeruginosa veterinary isolates recovered during 1994-2003 SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE antibiotics; disinfectants; fluoroquinolone antibiotics; organic acids; Pseudomonas aeruginosa; susceptibility ID PATHOGENICITY ISLAND PAPI-1; GRAM-NEGATIVE BACTERIA; ESCHERICHIA-COLI O157; METABOLITE CIPROFLOXACIN; ANTIMICROBIAL RESISTANCE; STAPHYLOCOCCUS-AUREUS; METHICILLIN-RESISTANT; MICROBIAL-FLORA; OTITIS-MEDIA; DAIRY HERDS AB AimsTo evaluate susceptibility of Pseudomonas aeruginosa veterinary isolates to antibiotics and disinfectants. Methods and ResultsPseudomonas aeruginosa isolates collected from dogs (n=155) and other animals (n=20) from sixteen states during 1994-2003 were tested for susceptibility. Most isolates were resistant to twenty-one antimicrobials tested, and the highest prevalence of resistance was to -lactams (938%) and sulphonamides (935%). Fluoroquinolone resistance did not increase from 1994 to 2003. Ciprofloxacin and enrofloxacin had a 5 and 16% prevalence of resistance, respectively, while sarafloxacin and nalidixic acid had a prevalence of resistance of 97 and 98%, respectively. Strains were pan-resistant to triclosan and chlorhexidine, were highly resistant to benzalkonium chloride and demonstrated high susceptibility to other disinfectants. Didecyldimethylammonium chloride was the most active ammonium chloride. Inducible resistance was observed to cetyl ammonium halides, chlorhexidine and benzyl ammonium chlorides, which formulate disinfectants used in veterinary clinics and dairies. Organic acid inhibition was associated with the dissociated acid species. ConclusionsDissociated organic acids appear able to inhibit Ps.aeruginosa, and rates of fluoroquinolone resistance merit sustained companion animal isolate surveillance. Significance and Impact of the StudyThis is the first report of Ps.aeruginosa susceptibility to 24 disinfectants and illustrates the high resistance of Ps.aeruginosa to both antibiotics and disinfectants. C1 [Beier, R. C.; Andrews, K.; Crippen, T. L.; Sheffield, C. L.; Poole, T. L.; Anderson, R. C.; Nisbet, D. J.] ARS, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. [Foley, S. L.] US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR USA. [Davidson, M. K.; White, D. G.; McDermott, P. F.; Bodeis-Jones, S.; Zhao, S.] US FDA, Res Off, Ctr Vet Med, Laurel, MD USA. RP Beier, RC (reprint author), ARS, Southern Plains Agr Res Ctr, USDA, 2881 F&B Rd, College Stn, TX 77845 USA. EM ross.beier@yahoo.com RI Grams, Michael/G-5197-2011 FU USDA, Agricultural Research Service FX The Authors thank Amir Karimloo and Seth Penny for technical support. This work was funded by the USDA, Agricultural Research Service. Mention of trade names, proprietary products or specific equipment is solely for the purpose of providing specific information and does not constitute a guarantee, warranty or endorsement by the U.S. Department of Agriculture or by the U.S. Food and Drug Administration and does not imply its approval to the exclusion of other products that may be suitable. Additionally, the views expressed in this article are those of the authors and do not necessarily reflect the official policy of the Department of Health and Human Services, the U.S. Food and Drug Administration or the U.S. Government. NR 86 TC 3 Z9 3 U1 6 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD FEB PY 2015 VL 118 IS 2 BP 326 EP 342 DI 10.1111/jam.12707 PG 17 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA4DM UT WOS:000348854500007 PM 25431276 ER PT J AU LaRocco, M Krug, PW Kramer, E Ahmed, Z Pacheco, JM Duque, H Baxt, B Rodriguez, LL AF LaRocco, Michael Krug, Peter W. Kramer, Ed Ahmed, Zaheer Pacheco, Juan M. Duque, Hernando Baxt, Barry Rodriguez, Luis L. TI A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine alpha(V)beta(6) Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus (vol 51, pg 1714, 2012) SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Correction C1 [LaRocco, Michael; Krug, Peter W.; Ahmed, Zaheer; Pacheco, Juan M.; Baxt, Barry; Rodriguez, Luis L.] USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, Greenport, NY 11944 USA. [Kramer, Ed] McConnell Grp Inc, Rockville, MD USA. [Duque, Hernando] USDA, Anim & Plant Hlth Inspect Serv, Plum Isl Anim Dis Ctr, Foreign Anim Dis Diagnost Lab, Greenport, NY USA. RP LaRocco, M (reprint author), USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, Greenport, NY 11944 USA. NR 1 TC 6 Z9 6 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD FEB PY 2015 VL 53 IS 2 BP 755 EP 755 PG 1 WC Microbiology SC Microbiology GA AZ9XU UT WOS:000348568500068 PM 25617444 ER PT J AU Garcia, M Elsasser, TH Biswas, D Moyes, KM AF Garcia, M. Elsasser, T. H. Biswas, D. Moyes, K. M. TI The effect of citrus-derived oil on bovine blood neutrophil function and gene expression in vitro SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE citrus oil; Holstein cow; bovine neutrophil ID ORANGE ESSENTIAL OIL; ANTIBACTERIAL ACTIVITY; MAMMARY-GLAND; MASTITIS; SALMONELLA; IMMUNITY; MECHANISMS; MONOCYTES; EXTRACTS; STRAINS AB Research on the use of natural products to treat or prevent microbial invasion as alternatives to antibiotic use is growing. Polymorphonuclear leukocytes (PMNL) play a vital role with regard to the innate immune response that affects severity or duration of mastitis. To our knowledge, effect of cold-pressed terpeneless Valencia orange oil (TCO) on bovine PMNL function has not been elucidated. Therefore, the objective of this study was to investigate the effect of TCO on bovine blood PMNL chemotaxis and phagocytosis capabilities and the expression of genes involved in inflammatory response in vitro. Polymorphonuclear leukocytes were isolated from jugular blood of 12 Holstein cows in mid-lactation and were incubated with 0.0 or 0.01% TCO for 120 min at 37 degrees C and 5% CO2, and phagocytosis (2 x 10(6) PMNL) and chemotaxis (6 x 10(6) PMNL) assays were then performed in vitro. For gene expression, RNA was extracted from incubated PMNL (6 x 10(6) PMNL), and gene expression was analyzed using quantitative PCR. The supernatant was stored at -80 degrees C for analysis of tumor necrosis factor-alpha. Data were analyzed using a general linear mixed model with cow and treatment (i.e., control or TCO) in the model statement. In vitro supplementation of 0.01% of TCO increased the chemotactic ability to IL-8 by 47%; however, migration of PMNL to complement 5a was not altered. Treatment did not affect the production of tumor necrosis factor-a by PMNL. Expression of proinflammatory genes (i.e., SELL, TLR4, IRAK1, TRAF6, and LYZ) coding for proteins was not altered by incubation of PMNL with TCO. However, downregulation of TLR2 [fold change (FC = treatment/control) = -2.14], NFKBIA (FC = 1.82), IL1B (FC = -2.16), TNFA (FC = -9.43), and SOD2 (FC = -1.57) was observed for PMNL incubated with TCO when compared with controls. Interestingly, expression of IL10, a well-known antiinflammatory cytokine, was also downregulated (FC = -3.78), whereas expression of IL8 (FC = 1.93), a gene coding for the cytokine IL-8 known for its chemotactic function, tended to be upregulated in PMNL incubated with TCO. Incubation of PMNL with TCO enhanced PMNL chemotaxis in vitro. The expression of genes involved in the inflammatory response was primarily downregulated. Results showed that 0.01% TCO did not impair the function of PMNL in vitro. Future studies investigating the use of TCO as an alternative therapy for treatment of mastitis, including dose and duration, for cows during lactation are warranted. C1 [Garcia, M.; Biswas, D.; Moyes, K. M.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Elsasser, T. H.] ARS, USDA, Growth Biol Lab, Beltsville, MD 20705 USA. RP Moyes, KM (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. EM kmoyes@umd.edu FU Maryland Agricultural Experiment Station FX This study was funded via the Maryland Agricultural Experiment Station. The authors acknowledge the Agricultural Research Service-USDA staff in Beltsville, Maryland, for their assistance with animal care and data collection. Gratitude in extended to Brian Barrows, Alejandro Almario, and Cori Williford, from University of Maryland, for their laboratory assistance. Firmenich Citrus Center (Safety Harbor, FL) is gratefully acknowledged for providing the citrus oil fraction. NR 46 TC 3 Z9 4 U1 3 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD FEB PY 2015 VL 98 IS 2 BP 918 EP 926 DI 10.3168/jds.2014-8450 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AZ2BK UT WOS:000348039300020 PM 25434342 ER PT J AU Koohmaraie, M Bosilevac, JM De La Zerda, M Motlagh, AM Samadpour, M AF Koohmaraie, Mohammad Bosilevac, Joseph M. De La Zerda, Michael Motlagh, Ali Mohseni Samadpour, Mansour TI Distribution of Escherichia coli Passaged through Processing Equipment during Ground Beef Production Using Inoculated Trimmings SO JOURNAL OF FOOD PROTECTION LA English DT Article ID UNITED-STATES; O157-H7; ILLNESS; GRINDER AB The contamination of raw ground beef by Escherichia coli O157:H7 is not only a public health issue but also an economic concern to meat processors. When E. coli O157:147 is detected in a ground beef sample, the product lots made immediately before and after the lot represented by the positive sample are discarded or diverted to lethality treatment. However, there is little data to base decisions on how much product must be diverted. Therefore, five 2,000-lb (907-kg) combo bins of beef trimmings were processed into 10-lb (4.54-kg) chubs of raw ground beef, wherein the second combo of meat was contaminated with a green fluorescent protein (GFP) expressing strain of E. coli. This was performed at two different commercial ground beef processing facilities, and at a third establishment where ground beef chubs from the second grinding establishment were mechanically split and repackaged into 3-lb (1.36-kg) loaves in trays. The GFP E. coli was tracked through the production of 10-lb (4.54-kg) chubs and the strain could not be detected after 26.5% more material (500 lb or 227 kg) and 87.8% more material (1,840 lb or 835 kg) followed the contaminated combo at each establishment, respectively. Three-pound (1.36-kg) loaves were no longer positive after just 8.6% more initially noncontaminated material (72 lb or 33 kg) was processed. The GFP strain could not be detected postprocessing in any residual meat or fat collected from the equipment used in the three trials. These results indicate that diversion to a safe end point (lethality or rendering) of the positive lot of ground beef, plus the lot before and lot after should remove contaminated ground beef, and as such provides support for the current industry practice. Further, the distribution and flow of E. coli on beef trimmings through various commercial equipment was different; thus, each establishment needs to consider this data when segregating lots of ground beef and establishing sampling protocols to monitor production. C1 [Koohmaraie, Mohammad; De La Zerda, Michael; Samadpour, Mansour] IEH Labs & Consulting Grp, Lake Forest Pk, WA 98155 USA. [Bosilevac, Joseph M.] USDA, ARS, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Motlagh, Ali Mohseni] Amer Foods Grp LLC, Green Bay, WI USA. RP Koohmaraie, M (reprint author), IEH Labs & Consulting Grp, 15300 Bothell Way N-E, Lake Forest Pk, WA 98155 USA. EM mk@iehinc.com NR 16 TC 1 Z9 1 U1 0 U2 7 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2015 VL 78 IS 2 BP 273 EP 280 DI 10.4315/0362-028X.JFP-14-369 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CA6YW UT WOS:000349064800005 PM 25710141 ER PT J AU Zhou, SY Sheen, S Pang, YH Liu, LS Yam, KL AF Zhou, Siyuan Sheen, Shiowshuh Pang, Yu-Hsin Liu, Linshu Yam, Kit L. TI Modeling the Impact of Vapor Thymol Concentration, Temperature, and Modified Atmosphere Condition on Growth Behavior of Salmonella on Raw Shrimp SO JOURNAL OF FOOD PROTECTION LA English DT Article ID BACTERIAL-GROWTH; ESSENTIAL OILS; TABLE GRAPES; QUALITY; COMBINATION; EUGENOL; SAFETY; PHASE; PH AB Salmonella is a microorganism of concern on a global basis for raw shrimp. This research modeled the impact of vapor thymol concentration (0, 0.8, and 1.6 mg/liter), storage temperature (8, 12, and 16 degrees C), and modified atmosphere condition (0.04 as in the natural atmosphere and 59.5% CO2) against the growth behavior of a Salmonella cocktail (six strains) on raw shrimp. Lag time (hour) and maximum growth rate (log CFU per gram per hour), chosen as two growth indicators, were obtained through DMFit software and then developed into polynomial as well as nonlinear modified secondary models (dimensional and/or dimensionless), consisting of two or even three impact factors in the equations. The models were validated, and results showed that the predictive values from both models demonstrated good matches to the observed experimental values, yet the prediction based on lag time was more accurate than maximum growth rate. The information will provide the food industry with insight into the potential safety risk of Salmonella growth on raw shrimp under stressed conditions. C1 [Zhou, Siyuan; Pang, Yu-Hsin; Yam, Kit L.] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA. [Sheen, Shiowshuh; Liu, Linshu] USDA, ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), USDA, ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM Shiowshuh.sheen@ars.usda.gov NR 29 TC 3 Z9 3 U1 0 U2 7 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2015 VL 78 IS 2 BP 293 EP 301 DI 10.4315/0362-028X.JFP-14-264 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CA6YW UT WOS:000349064800008 PM 25710144 ER PT J AU Mohamed, TM Parveen, S Ludwig, JB Oscar, TP AF Mohamed, T. M. Parveen, S. Ludwig, J. B. Oscar, T. P. TI Chlorine Inactivation of Salmonella Kentucky Isolated from Chicken Carcasses: Evaluation of Strain Variation SO JOURNAL OF FOOD PROTECTION LA English DT Article ID BROILER CARCASSES; POULTRY; TYPHIMURIUM; CAMPYLOBACTER; WATER AB The current study was undertaken to evaluate chlorine resistance among strains of Salmonella Kentucky isolated from chicken carcasses. Selected strains (n = 8) were exposed to 30 ppm of chlorine in 10% buffered peptone water (pH 7.4) for 0 to 10 min at 4 degrees C and 150 rpm. The initial level (mean +/- SD) of Salmonella Kentucky was 6.18 +/- 0.09 log CFU/ml and did not differ (P > 0.05) among strains. A two-way analysis of variance indicated that the level of Salmonella Kentucky in chlorinated water was affected (P < 0.05) by a time by strain interaction. Differences among strains increased as a function of chlorine exposure time. After 10 min of chlorine exposure, the most resistant strain (SK145) was 5.63 +/- 0.54 log CFU/ml, whereas the least resistant strain (SK275) was 3.07 +/- 0.29 log CFU/ml. Significant differences in chlorine resistance were observed for most strain comparisons. Death of Salmonella Kentucky was nonlinear over time and fitted well to a power law model with a shape parameter of 0.34 (concave upward). Time (minutes) for a 1-log reduction of Salmonella Kentucky differed (P < 0.05) among strains: >10 mm for SK145, 6.0 min for SK254, 1.5 min for SK179, and 0.3 to 0.65 min for other strains. Results of this study indicate that strain is an important :variable to include in models that predict changes in levels of Salmonella Kentucky in chlorinated water. C1 [Mohamed, T. M.; Parveen, S.] Univ Maryland Eastern Shore, Dept Agr Food & Resource Sci, Food Sci & Technol Program, Princess Anne, MD 21853 USA. [Ludwig, J. B.; Oscar, T. P.] Univ Maryland Eastern Shore, USDA, Residue Chem & Predict Microbiol Res Unit, Ctr Food Sci & Technol,ARS, Princess Anne, MD 21853 USA. RP Parveen, S (reprint author), Univ Maryland Eastern Shore, Dept Agr Food & Resource Sci, Food Sci & Technol Program, Princess Anne, MD 21853 USA. EM sparveen@umes.edu FU USDA Evans-Allen program FX The authors thank Perdue Foods LLC (Salisbury, MD) for providing the chlorine and the USDA Evans-Allen program for funding the project. NR 13 TC 0 Z9 0 U1 0 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2015 VL 78 IS 2 BP 414 EP 418 DI 10.4315/0362-028X.JFP-14-379 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CA6YW UT WOS:000349064800024 PM 25710160 ER PT J AU Gunther, NW Rajkowski, KT Sommers, C AF Gunther, Nereus W. Rajkowski, Kathleen T. Sommers, Christopher TI Survival after Cryogenic Freezing of Campylobacter Species in Ground Turkey Patties Treated with Polyphosphates SO JOURNAL OF FOOD PROTECTION LA English DT Article ID LISTERIA-MONOCYTOGENES; CHICKEN MEAT; JEJUNI; PATHOGENS; STORAGE; FROZEN; SALMONELLA; POULTRY; COLI; PH AB The use of polyphosphate-based marinades in the processing of poultry has been previously shown to increase the survival of Campylobacter species present in the exudates derived from these products. This study investigates the effects that some of the same polyphosphates have on the survival of Campylobacter species within a ground turkey product subjected to cryogenic freezing. Ground turkey patties with two different polyphosphate formulations added in two different concentrations were artificially contaminated with known concentrations of Campylobacter jejuni or Campylobacter colt. The patties were cryogenically frozen at -80 degrees F (-62.2 degrees C) with liquid nitrogen vapor and held at -20 degrees C for 7 or 33 days, after which the number of Campylobacter surviving in the patties was determined. On average the cryogenic freezing resulted in a 2.5-log decrease in the survival of C. jejuni cells and a 2.9-log decrease in C. coli cells present in the turkey patties. Additionally, the presence of polyphosphates in the turkey patties had no effect on Campylobacter survival up to the maximum allowed concentration (0.5%) for polyphosphates in poultry marinades. Finally, it was determined that the added polyphosphates had little effect on the pH of the ground turkey meat; an effect which previously had been implicated in the enhancement of Campylobacter survival due to the presence of polyphosphates. C1 [Gunther, Nereus W.] USDA, ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, Wyndmoor, PA 19038 USA. [Rajkowski, Kathleen T.; Sommers, Christopher] USDA, ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Gunther, NW (reprint author), USDA, ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM jack.gunther@ars.usda.gov NR 29 TC 1 Z9 1 U1 2 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2015 VL 78 IS 2 BP 419 EP 423 DI 10.4315/0362-028X.JFP-14-301 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CA6YW UT WOS:000349064800025 PM 25710161 ER PT J AU Guo, M Dubey, JP Hill, D Buchanan, RL Gamble, HR Jones, JL Pradhan, AK AF Guo, Miao Dubey, Jitender P. Hill, Dolores Buchanan, Robert L. Gamble, H. Ray Jones, Jeffrey L. Pradhan, Abani K. TI Prevalence and Risk Factors for Toxoplasma gondii Infection in Meat Animals and Meat Products Destined for Human Consumption SO JOURNAL OF FOOD PROTECTION LA English DT Review ID FREE-RANGE CHICKENS; NEOSPORA-CANINUM ANTIBODIES; SAO-PAULO-STATE; POLYMERASE-CHAIN-REACTION; MODIFIED AGGLUTINATION-TEST; CROSS-SECTIONAL SURVEY; FERNANDO-DE-NORONHA; PIG BREEDING FARMS; UNITED-STATES; DOMESTIC PIGS AB Toxoplasma gondii is a protozoan parasite that is responsible for approximately 24% of all estimated deaths attributed to foodborne pathogens in the United States. Human infection results from accidental ingestion of oocysts from the environment, in water, or on insufficiently washed produce or from consumption of raw or undercooked meat products that contain T. gondii tissue cysts. This review focused on studies of T. gondii in meat because many human T. gondii infections are acquired through consumption of raw or undercooked meat. Prevalence of T. gondii is higher in conventionally reared pigs, sheep, and poultry than in cattle and is greater in meat products from organic than from conventionally reared meat animals because of outdoor access, which poses substantially greater opportunities for exposure to infected rodents, wildlife, and oocyst-contaminated feed, water, or environmental surfaces. Risk factors related to T. gondii exposure for livestock include farm type, feed source, presence of cats, methods of rodent and bird control, methods of carcass handling, and water quality. This review serves as a useful resource and information repository for informing quantitative risk assessment studies for T. gondii infection in humans through meat consumption. C1 [Guo, Miao; Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Dubey, Jitender P.; Hill, Dolores] USDA, ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Gamble, H. Ray] Natl Acad Sci, Fellowships Off, Washington, DC 20001 USA. [Jones, Jeffrey L.] Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. RP Pradhan, AK (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM akp@umd.edu FU USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative [2012-67005-19611] FX This work was supported through a grant from the USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (award 2012-67005-19611). NR 253 TC 14 Z9 16 U1 7 U2 50 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD FEB PY 2015 VL 78 IS 2 BP 457 EP 476 DI 10.4315/0362-028X.JFP-14-328 PG 20 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA CA6YW UT WOS:000349064800030 PM 25710166 ER PT J AU Costello, RB Saldanha, L Dwyer, J Andrews, K Bailen, R Bailey, R Betz, J Burt, V Chang, F Emenaker, N Gahche, J Hardy, C Pehrsson, P AF Costello, R. B. Saldanha, L. Dwyer, J. Andrews, K. Bailen, R. Bailey, R. Betz, J. Burt, V Chang, F. Emenaker, N. Gahche, J. Hardy, C. Pehrsson, P. TI NEW NATIONAL INSTITUTES OF HEALTH DIETARY SUPPLEMENT LABEL DATABASE CAN AID SEARCH FOR INGREDIENT INFORMATION SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Meeting Abstract CT Southern Regional Meeting of the American-Federation-for-Medical-Research CY FEB 26-28, 2015 CL New Orleans, LA SP Amer Federat Med Res C1 [Costello, R. B.; Saldanha, L.; Dwyer, J.; Bailen, R.; Bailey, R.; Betz, J.; Chang, F.; Emenaker, N.] NIH, Bethesda, MD 20892 USA. [Andrews, K.; Pehrsson, P.] USDA, Beltsville, MD 20705 USA. [Burt, V; Gahche, J.] Ctr Dis Control & Prevent, Hyattsville, MD USA. [Hardy, C.] US FDA, College Pk, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1081-5589 EI 1708-8267 J9 J INVEST MED JI J. Invest. Med. PD FEB PY 2015 VL 63 IS 2 MA 218 BP 386 EP 386 PG 1 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA CA0GM UT WOS:000348595000229 ER PT J AU Hair, AB Peluso, AM Hawthorne, KM Khan, JY O'Donnell, A Powers, RJ Lee, ML Abrams, SA AF Hair, A. B. Peluso, A. M. Hawthorne, K. M. Khan, J. Y. O'Donnell, A. Powers, R. J. Lee, M. L. Abrams, S. A. TI NECROTIZING ENTEROCOLITIS AND BEYOND: IMPROVING OUTCOMES WITH AN EXCLUSIVE HUMAN MILK-BASED DIET SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Meeting Abstract CT Southern Regional Meeting of the American-Federation-for-Medical-Research CY FEB 26-28, 2015 CL New Orleans, LA SP Amer Federat Med Res C1 [Hair, A. B.; Peluso, A. M.; Hawthorne, K. M.; Abrams, S. A.] Texas Childrens Hosp, USDA ARS Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX 77030 USA. [Khan, J. Y.] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. [O'Donnell, A.] NW Mem Hosp, Chicago, IL 60611 USA. [Powers, R. J.] Good Samaritan Hosp, Pediat Med Grp, San Jose, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1081-5589 EI 1708-8267 J9 J INVEST MED JI J. Invest. Med. PD FEB PY 2015 VL 63 IS 2 MA 520 BP 474 EP 475 PG 2 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA CA0GM UT WOS:000348595000527 ER PT J AU Ip, BC Liu, C Lichtenstein, AH von Lintig, J Wang, XD AF Ip, Blanche C. Liu, Chun Lichtenstein, Alice H. von Lintig, Johannes Wang, Xiang-Dong TI Lycopene and Apo-10 '-lycopenoic Acid Have Differential Mechanisms of Protection against Hepatic Steatosis in beta-Carotene-9 ',10 '-oxygenase Knockout Male Mice SO JOURNAL OF NUTRITION LA English DT Article DE nonalcoholic fatty liver disease; hepatic cholesterol; lipid metabolism; sirtuin 1; obesity; plasma lipids; mitochondrial uncoupling; PPAR; carotenoid metabolism; mesenteric adipose tissue ID FATTY LIVER-DISEASE; INSULIN-RESISTANCE; LIPID-METABOLISM; PPAR-GAMMA; NONALCOHOLIC STEATOHEPATITIS; CARDIOVASCULAR-DISEASE; SUBSTRATE-SPECIFICITY; SIRT1 DEACETYLATES; NUCLEAR RECEPTOR; DIETARY LYCOPENE AB Background: Nonalcoholic fatty liver disease is positively associated with obesity and cardiovascular disease risk. Apo-10'-lycopenoic acid (APO10LA), a potential oxidation product of apo-10'-lycopenal that is generated endogenously by beta-carotene-9',10'-oxygenase (BCO2) cleavage of lycopene, inhibited hepatic steatosis in BCO2-expressing mice. Objective: The present study evaluated lycopene and APO10LA effects on hepatic steatosis in mice without BCO2 expression. Methods: Male and female BCO2-knockout (BCO2-KO) mice were fed a high saturated fat diet (HSFD) with or without APO10LA (10 mg/kg diet) or lycopene (100 mg/kg diet) for 12 wk. Results: Lycopene or APO10LA supplementation reduced hepatic steatosis incidence (78% and 72%, respectively) and severity in BCO2-KO male mice. Female mice did not develop steatosis, had greater hepatic total cholesterol (3.06 vs. 2.31 mg/g tissue) and cholesteryl ester (1.58 vs. 0.86 mg/g tissue), but had lower plasma triglyceride (TG) 1229 vs. 282 mg/dL) and cholesterol (97.1 vs. 119 mg/dL) than male mice. APO10LA-mitigated steatosis in males was associated with reduced hepatic total cholesterol (18%) and activated sirtuin 1 signaling, which resulted in reduced fatty acids (FAs) and TG synthesis markers [stearoyl-coenzyme A (CoA) desaturase protein, 71%; acetyl-CoA carboxylase phosphorylation, 79%; AMP-activated protein kinase phosphorylation, 67%1, and elevated cholesterol efflux genes (cytochrome P450 family 7A1, 65%; ATP-binding cassette transporter G5/8, 11%). These APO10LA-mediated effects were not mimicked by lycopene supplementation. Intriguingly, steatosis inhibition by lycopene induced peroxisome proliferator activated receptor (PPAR)alpha- and PPAR gamma-related genes in mesenteric adipose tissue (MAT) that increases mitochondria! uncoupling [cell death inducing DNA fragmentation factor, a subunit-like effector a, 55%; PR domain-containing 16, 47%; uncoupling protein 3 (Ucp3), 55%], FA beta-oxidation (PPAR alpha, 53%; very long chain acyl-CoA dehydrogenase; 38%), and uptake (FA transport protein 4, 29%; lipoprotein lipase 43%). Expressions of 10 MAT PPAR-related genes were inversely correlated with steatosis score, suggesting that lycopene reduced steatosis by increasing MAT FA utilization. Conclusions: Our data suggest that lycopene and APO10LA inhibit HSFD-induced steatosis in BCO2-KO male mice through differential mechanisms. Sex disparity of BCO2-KO mice was observed in the outcomes of HSFD-induced liver steatosis and plasma lipids. C1 [Ip, Blanche C.; Liu, Chun; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, Boston, MA 02111 USA. [Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lichtenstein, Alice H.; Wang, Xiang-Dong] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [von Lintig, Johannes] Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA. RP Wang, XD (reprint author), Tufts Univ, Nutr & Canc Biol Lab, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu FU NIH [CA104932]; USDA/Agricultural Research Service [1950-51000-074S]; NIH/National Heart, Lung, and Blood Institute [5T32HL069772-10, 2T32HL069772-11A1] FX Supported by NIH grant CA104932, USDA/Agricultural Research Service grant 1950-51000-074S, and NIH/National Heart, Lung, and Blood Institute grants 5T32HL069772-10 and 2T32HL069772-11A1. Any opinions, findings, conclusions, and recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the sponsors. NR 64 TC 5 Z9 5 U1 0 U2 10 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD FEB PY 2015 VL 145 IS 2 BP 268 EP 276 DI 10.3945/jn.114.200238 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA6WT UT WOS:000349057200012 PM 25644347 ER PT J AU Rahavi, E Stoody, EE Rihane, C Casavale, KO Olson, R AF Rahavi, Elizabeth Stoody, Eve Essery Rihane, Colette Casavale, Kellie O. Olson, Richard TI Updating the Dietary Guidelines for Americans: Status and Looking Ahead SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Rahavi, Elizabeth; Stoody, Eve Essery; Rihane, Colette] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. [Casavale, Kellie O.; Olson, Richard] US Dept HHS, Div Prevent Sci, Off Dis Prevent & Hlth Promot, Rockville, MD USA. RP Rahavi, E (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 0 TC 0 Z9 2 U1 1 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2015 VL 115 IS 2 BP 180 EP 182 DI 10.1016/j.jand.2014.11.022 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA1YC UT WOS:000348703900002 PM 25636216 ER PT J AU Dawson-Hughes, B Harris, SS Lichtenstein, AH Dolnikowski, G Palermo, NJ Rasmussen, H AF Dawson-Hughes, Bess Harris, Susan S. Lichtenstein, Alice H. Dolnikowski, Gregory Palermo, Nancy J. Rasmussen, Helen TI Dietary Fat Increases Vitamin D-3 Absorption SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Vitamin D-3; Absorption; Vitamin D-3 supplementation; Meal conditions; Healthy adults ID D-BINDING PROTEIN; POSTMENOPAUSAL WOMEN; D SUPPLEMENTATION AB Background The plasma 25-hydroxyvitamin D response to supplementation with vitamin D varies widely, but vitamin D absorption differences based on diet composition is poorly understood. Objectives We tested the hypotheses that absorption of vitamin D-3 is greater when the supplement is taken with a meal containing fat than with a fat-free meal and that absorption is greater when the fat in the meal has a higher monounsaturated-to-polyunsaturated fatty acid ratio (MUFA:PUFA). Design Open, three-group, single-dose vitamin D-3 comparative absorption experiment. Participants/setting Our 1-day study was conducted in 50 healthy older men and women who were randomly assigned to one of three meal groups: fat-free meal, and a meal with 30% of calories as fat with a low (1:4) and one with a high (4:1) MUFA: PUFA. After a 12-hour fast, all subjects took a single 50,000 IU vitamin D-3 supplement with their test breakfast meal. Main outcome measures Plasma vitamin D-3 was measured by liquid chromatography emass spectrometry before and 10, 12 (the expected peak), and 14 hours after the dose. Statistical analyses performed Means were compared with two-tailed t tests for independent samples. Group differences in vitamin D-3 absorption across the measurement time points were examined by analysis of variance with the repeated measures subcommand of the general linear models procedure. Results The mean peak (12-hour) plasma vitamin D-3 level after the dose was 32% (95% CI 11% to 52%) greater in subjects consuming fat-containing compared with fat-free meals (P = 0.003). Absorption did not differ significantly at any time point in the high and low MUFA and PUFA groups. Conclusions The presence of fat in a meal with which a vitamin D-3 supplement is taken significantly enhances absorption of the supplement, but the MUFA: PUFA of the fat in that meal does not influence its absorption. C1 [Dawson-Hughes, Bess] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Dawson-Hughes, Bess] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging HNRCA, Bone Metab Lab, Boston, MA 02111 USA. [Harris, Susan S.] Tufts Univ, Friedman Sch Nutr, Boston, MA 02111 USA. [Harris, Susan S.; Palermo, Nancy J.] Tufts Univ, HNRCA, Bone Metab Lab, Boston, MA 02111 USA. [Lichtenstein, Alice H.; Rasmussen, Helen] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Lichtenstein, Alice H.] Tufts Univ, HNRCA, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Dolnikowski, Gregory] Tufts Univ, HNRCA, Mass Spectrometry Unit, Boston, MA 02111 USA. [Rasmussen, Helen] Tufts Univ, HNRCA, Metab Res Unit, Boston, MA 02111 USA. RP Dawson-Hughes, B (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM bess.dawson-hughes@tufts.edu FU Pfizer [WS2105602]; USDA Human Nutrition Research Center on Aging at Tufts University; US Department of Agriculture [58-1950-0-014] FX This study was cofunded by an Investigator-Initiated Research Grant from Pfizer (IIR grant no. WS2105602) and a pilot grant from USDA Human Nutrition Research Center on Aging at Tufts University. This work is supported in part by the US Department of Agriculture under agreement no. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. NR 20 TC 8 Z9 8 U1 1 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2015 VL 115 IS 2 BP 225 EP 230 DI 10.1016/j.jand.2014.09.014 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA1YC UT WOS:000348703900008 PM 25441954 ER PT J AU Troup, R Hayes, JH Raatz, SK Thyagarajan, B Khaliq, W Jacobs, DR Key, NS Morawski, BM Kaiser, D Bank, AJ Gross, M AF Troup, Rasa Hayes, Jennifer H. Raatz, Susan K. Thyagarajan, Bharat Khaliq, Waseem Jacobs, David R., Jr. Key, Nigel S. Morawski, Bozena M. Kaiser, Daniel Bank, Alan J. Gross, Myron TI Effect of Black Tea Intake on Blood Cholesterol Concentrations in Individuals with Mild Hypercholesterolemia: A Diet-Controlled Randomized Trial SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Black tea; Flavonoids; Hypercholesterolemia; Randomized crossover controlled trial; Serum lipids ID LOW-DENSITY-LIPOPROTEIN; DECREASE MICELLAR SOLUBILITY; CORONARY-HEART-DISEASE; FREE-LIVING SUBJECTS; GREEN TEA; SERUM-CHOLESTEROL; INTESTINAL-ABSORPTION; PLASMA-CHOLESTEROL; CHINESE TEA; CRUDE DRUGS AB Habitual intake of black tea has been associated with relatively lower serum cholesterol concentrations in observational studies. However, clinical trial results evaluating the effects of black tea on serum cholesterol have been inconsistent. Several factors could explain these mixed results, in particular, uncontrolled confounding caused by lifestyle factors (eg, diet). This diet-controlled clinical trial estimates the effect of black tea flavonoid consumption on cholesterol concentrations in 57 borderline hypercholesterolemic individuals (total cholesterol concentrations between 190 and 260 mg/dL [4.9 and 6.7 mmol/L]). A double-blind, randomized crossover trial was conducted in Minneapolis, MN, from April 2002 through April 2004 in which key conditions were tightly controlled to minimize possible confounding. Participants consumed a controlled low-flavonoid diet plus 5 cups per day of black tea or tea-like placebo during two 4-week treatment periods. The flavonoid-free caffeinated placebo matched the tea in color and taste. Differences in cholesterol concentrations at the end of each treatment period were evaluated via linear mixed models. Differences among those treated with tea vs placebo were 3.43 mg/dL (0.09 mmol/L) (95% CI -7.08 to 13.94) for total cholesterol, -1.02 mg/dL (-0.03 mmol/L) (95% CI -11.34 to 9.30) for low-density lipoprotein cholesterol, 0.58 mg/dL (0.02 mmol/L) (95% CI -2.98 to 4.14) for high-density lipoprotein cholesterol, 15.22 mg/dL (0.17 mmol/L) (95% CI -40.91 to 71.35) for triglycerides, and -0.39 mg/dL (-0.01 mmol/L) (95% CI -11.16 to 10.38) for low-density lipoprotein plus high-density lipoprotein cholesterol fraction. The low-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio decreased by -0.1 units (95% CI -0.41 to 0.21). No results were statistically or clinically significant. The intake of 5 cups of black tea per day did not alter the lipid profile of borderline hypercholesterolemic subjects significantly. C1 [Troup, Rasa] Univ Minnesota, Dept Intercollegiate Athlet, Minneapolis, MN 55455 USA. [Troup, Rasa] Viverant, St Louis Pk, MN USA. [Troup, Rasa] Univ Minnesota, Dept Nutr, St Paul, MN 55108 USA. [Troup, Rasa; Hayes, Jennifer H.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Hayes, Jennifer H.] Maryland Dept Hlth & Mental Hyg, Maryland Canc Registry, Baltimore, MD USA. [Raatz, Susan K.] ARS, USDA, Grand Forks Human Nutr Res Ctr, North Grand Forks, ND USA. [Raatz, Susan K.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN USA. [Raatz, Susan K.] Univ Minnesota, Gen Clin Res Ctr, Minneapolis, MN 55455 USA. [Raatz, Susan K.; Key, Nigel S.] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA. [Thyagarajan, Bharat; Morawski, Bozena M.; Gross, Myron] Univ Minnesota, Sch Med, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [Thyagarajan, Bharat; Jacobs, David R., Jr.; Morawski, Bozena M.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55455 USA. [Khaliq, Waseem] Johns Hopkins Univ, Sch Med, Johns Hopkins Bayview Med Ctr, Baltimore, MD USA. [Khaliq, Waseem; Jacobs, David R., Jr.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol, Minneapolis, MN 55455 USA. [Key, Nigel S.] Univ N Carolina, Dept Med, UNC Hemophilia & Thrombosis Ctr, Chapel Hill, NC USA. [Key, Nigel S.] Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC USA. [Kaiser, Daniel] Greatbatch Inc, Frisco, TX USA. [Kaiser, Daniel; Bank, Alan J.] St Paul Heart Clin, St Paul, MN USA. [Bank, Alan J.] United Heart & Vasc Clin, St Paul, MN USA. [Bank, Alan J.] Univ Minnesota, Sch Med, Dept Med, Div Cardiol, Minneapolis, MN 55455 USA. [Gross, Myron] Univ Minnesota, Sch Med, Mol Epidemiol & Biomarker Res Lab, Minneapolis, MN 55455 USA. [Gross, Myron] Univ Minnesota, Sch Med, Mol Epidemiol & Biomarker Res Lab, Minneapolis, MN 55455 USA. RP Gross, M (reprint author), Univ Minnesota, Sch Med, Dept Lab Med & Pathol, MMC 609,420 Delaware St SE, Minneapolis, MN 55455 USA. EM gross001@umn.edu OI Morawski, Bozena/0000-0003-1655-6006 FU Lipton Tea Company/Unilever-Best Foods NA; Minnesota Medical Foundation; National Institutes of Health [M01-RR-00400] FX This research was supported by the Lipton Tea Company/Unilever-Best Foods NA, Minnesota Medical Foundation, and National Institutes of Health grant no. M01-RR-00400 of the National Center for Research Resources. NR 59 TC 1 Z9 1 U1 1 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2015 VL 115 IS 2 BP 264 EP U1122 DI 10.1016/j.jand.2014.07.021 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA1YC UT WOS:000348703900012 PM 25266246 ER PT J AU Sebastian, RS Enns, CW Goldman, JD Hoy, MK Moshfegh, AJ AF Sebastian, Rhonda S. Enns, Cecilia Wilkinson Goldman, Joseph D. Hoy, Mary K. Moshfegh, Alanna J. TI Sandwiches Are Major Contributors of Sodium in the Diets of American Adults: Results from What We Eat in America, National Health and Nutrition Examination Survey 2009-2010 SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Sandwich consumption; Sodium intake; Sodium density; Adults; What We Eat in America, National Health and Nutrition Examination Survey ID UNITED-STATES; PHYSICAL-ACTIVITY; FOOD SOURCES; PATTERNS; ENERGY; IMPACT AB Efforts to sharpen the focus of sodium reduction strategies include identification of major food group contributors of sodium intake. Although sandwiches are a staple of the American diet, previous examinations of their contribution to sodium intake captured only a small subset of sandwiches. One day of dietary intak e data from 5,762 adults aged 20 years and older in What We Eat in America, National Health and Nutrition Examination Survey 2009-2010 was analyzed. Sandwiches were defined in a manner that more accurately reflected their frequency of consumption. Two-sided t tests were used to compare percentages of men and women reporting sandwiches; contributions of sandwiches to energy and sodium intakes (amounts in kilocalories and milligrams, respectively, and percent of daily totals) by sex; and total energy, total sodium, and sodium density (mg/1,000 kcal) by sandwich reporting status (reporter/nonreporter). On any given day, 49% of American adults ate sandwiches. A significantly higher percentage of men than women reported sandwiches (54% vs 44%, respectively; P<0.001), and sandwiches accounted for higher percentages of men's total energy and sodium intakes. Compared with individuals who did not report a sandwich on the intake day, sandwich reporters had significantly higher energy and sodium intakes; however, sodium density of the diet did not vary by sandwich reporting status. Although much national attention is appropriately focused on reducing sodium in the food supply, consumer choices still play a vital role. Due to sandwiches' frequent consumption and considerable contributions to sodium intake, substituting lower-sodium for higher-sodium ingredients in sandwiches could significantly influence sodium intakes. C1 [Sebastian, Rhonda S.; Enns, Cecilia Wilkinson; Goldman, Joseph D.; Hoy, Mary K.; Moshfegh, Alanna J.] ARS, USDA, Food Surveys Res Grp, Beltsville, MD 20705 USA. RP Sebastian, RS (reprint author), ARS, USDA, Food Surveys Res Grp, 10300 Baltimore Ave,Bldg 005,Room 102,BARC West, Beltsville, MD 20705 USA. EM Rhonda.Sebastian@ars.usda.gov NR 27 TC 2 Z9 2 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD FEB PY 2015 VL 115 IS 2 BP 272 EP 277 DI 10.1016/j.jand.014.07.034 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA1YC UT WOS:000348703900013 PM 25300226 ER PT J AU Chitwood, MC Lashley, MA Kilgo, JC Moorman, CE Deperno, CS AF Chitwood, Michael C. Lashley, Marcus A. Kilgo, John C. Moorman, Christopher E. Deperno, Christopher S. TI White-Tailed Deer Population Dynamics and Adult Female Survival in the Presence of a Novel Predator SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE coyote; elasticity; neonate; population dynamics; sensitivity; survival; white-tailed deer ID COYOTE PREDATION; SENSITIVITY ANALYSES; ELASTICITY ANALYSIS; TEMPORAL VARIATION; LARGE HERBIVORES; NORTH-AMERICA; UNITED-STATES; CONSERVATION; GROWTH; FAWNS AB Recent localized declines in white-tailed deer (Odocoileus virginianus) populations in the southeastern United States have been linked to increasing predation pressure from coyotes (Canis latrans), a novel predator to the region. Studies have documented coyotes as the leading cause of mortality for neonates, and 1 study documented coyotes as a mortality factor for adult females. However, no study has used field-based vital rates to conduct sensitivity analyses or model deer population trajectories under potential harvest or predator removal strategies. We used low, medium, and high values of fawn survival, adult female survival, and fecundity data collected from Fort Bragg Military Installation, North Carolina to demonstrate the current declining population trajectory for deer (=0.905; low =0.788, high =1.003). Consistent with other studies of ungulates, we determined adult female survival was the most sensitive and elastic vital rate. Further, for 3 potential management (what if) scenarios, we projected the population for 10 years using estimated vital rates. Reducing adult female harvest (=0.935; low =0.875, high =1.002) and coyote removal (=0.995; low =0.898, high =1.081) reduced the current population decline, whereas combining both approaches (=1.024; low =0.898, high =1.141) resulted in population increases. Our data indicate that for low-density deer populations with heavy predation pressure on neonates, protecting adult females from harvest may not completely offset population declines. Coyote removal might be a necessary strategy because it could possibly increase very low fawn survival, which appears to be the most important vital rate influencing in our study. However, managers may have to start with reductions in adult female harvest because coyote removal would have to be continuous and consistently effective, making it an impractical management approach by itself. (c) 2015 The Wildlife Society. C1 [Chitwood, Michael C.; Lashley, Marcus A.; Moorman, Christopher E.; Deperno, Christopher S.] N Carolina State Univ, Dept Forestry & Environm Resources, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. [Kilgo, John C.] US Forest Serv, USDA, Southern Res Stn, New Ellenton, SC 29809 USA. RP Chitwood, MC (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. EM colter_chitwood@ncsu.edu FU United States Department of Defense; Fort Bragg Wildlife Branch FX We thank the United States Department of Defense and Fort Bragg Wildlife Branch for funding. We thank A. Schultz, J. Jones, C. Brown, and J. Heisinger for logistical support. We thank M. Broadway, B. Sherrill, K. Young, M. Nunnery, B. Peterson, A. Schrader, A. Murkowski, and C. Farr for help in the field. We thank A. Kumar, S. Mills, and K. Pollock for help with data analyses. We thank K. Pollock and all anonymous referees for providing helpful comments on earlier drafts of the manuscript. NR 61 TC 10 Z9 10 U1 13 U2 73 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2015 VL 79 IS 2 BP 211 EP 219 DI 10.1002/jwmg.835 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CA0YO UT WOS:000348639700005 ER PT J AU Sovern, SG Forsman, ED Dugger, KM Taylor, M AF Sovern, Stan G. Forsman, Eric D. Dugger, Katie M. Taylor, Margaret TI Roosting Habitat Use and Selection By Northern Spotted Owls During Natal Dispersal SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE dispersal habitat; logistic regression; northern spotted owl; radio-telemetry; Strix occidentalis caurina; Washington ID STRIX-OCCIDENTALIS-CAURINA; HOME-RANGE; WESTERN OREGON; DEMOGRAPHIC PERFORMANCE; EASTERN WASHINGTON; RESOURCE SELECTION; CANOPY COVER; BARRED OWLS; SPACE-USE; FORESTS AB We studied habitat selection by northern spotted owls (Strix occidentalis caurina) during natal dispersal in Washington State, USA, at both the roost site and landscape scales. We used logistic regression to obtain parameters for an exponential resource selection function based on vegetation attributes in roost and random plots in 76 forest stands that were used for roosting. We used a similar analysis to evaluate selection of landscape habitat attributes based on 301 radio-telemetry relocations and random points within our study area. We found no evidence of within-stand selection for any of the variables examined, but 78% of roosts were in stands with at least some large (>50cmdbh) trees. At the landscape scale, owls selected for stands with high canopy cover (>70%). Dispersing owls selected vegetation types that were more similar to habitat selected by adult owls than habitat that would result from following guidelines previously proposed to maintain dispersal habitat. Our analysis indicates that juvenile owls select stands for roosting that have greater canopy cover than is recommended in current agency guidelines. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Sovern, Stan G.; Taylor, Margaret] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Forsman, Eric D.] US Forest Serv, USDA, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. [Dugger, Katie M.] Oregon State Univ, Dept Fisheries & Wildlife, Oregon Cooperat Fish & Wildlife Res Unit, US Geol Survey, Corvallis, OR 97331 USA. RP Sovern, SG (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. EM ssovern@fs.fed.us FU United States Department of Agriculture Forest Service, Pacific Northwest Regional Office and Pacific Northwest Research Station; United States Department of Interior (USDI) Bureau of Land Management, Oregon State Office; USDI United States Geological Survey, Biological Resources Division, Forest and Rangeland Ecosystem Science Center, Corvallis, Oregon; USDI Fish and Wildlife Service FX We are especially grateful to V. Illg, D. Manson, K. Maurice, and S. Smith for their dedication and perseverance in tracking owls, often under harsh field conditions. J. Bottelli, D. Herter, L. Hicks, C. Holloway, D. Maulkin, L. Melampy, A. Renkert, and H. Stabins at Plum Creek Timber Company also helped track owls. B. Biswell developed the plot methodology. J. Jones, M. Bieker, and J. Haddon provided harvest data for private and state lands. M. Huso, G. Olson, and M. Raphael provided guidance in the early stages of data analysis. P. Singleton provided the data layers for topographic position, solar insolation, and slope. R. Gutierrez, P. Singleton, and 3 anonymous reviewers provided helpful comments on earlier drafts of the manuscript. E. Merrill provided helpful insight into resource selection functions. Funding was provided by the United States Department of Agriculture Forest Service, Pacific Northwest Regional Office and Pacific Northwest Research Station; United States Department of Interior (USDI) Bureau of Land Management, Oregon State Office; and USDI United States Geological Survey, Biological Resources Division, Forest and Rangeland Ecosystem Science Center, Corvallis, Oregon. The USDI Fish and Wildlife Service provided funding for aircraft searches. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 58 TC 1 Z9 1 U1 5 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2015 VL 79 IS 2 BP 254 EP 262 DI 10.1002/jwmg.834 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CA0YO UT WOS:000348639700009 ER PT J AU Latif, QS Saab, VA Mellen-Mclean, K Dudley, JG AF Latif, Quresh S. Saab, Victoria A. Mellen-Mclean, Kim Dudley, Jonathan G. TI Evaluating Habitat Suitability Models for Nesting White-Headed Woodpeckers in Unburned Forest SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE forest restoration; Mahalanobis D-2; Maxent; model validation; Picoides albolarvatus; presence-only models; species distributions ID SPECIES DISTRIBUTION MODELS; PRESENCE-ONLY DATA; DISTRIBUTIONS; CONSERVATION; PREDICTION; ECOLOGY; TRANSFERABILITY; PERFORMANCE; LANDSCAPE; NORTHWEST AB Habitat suitability models can provide guidelines for species conservation by predicting where species of interest are likely to occur. Presence-only models are widely used but typically provide only relative indices of habitat suitability (HSIs), necessitating rigorous evaluation often using independently collected presence-absence data. We refined and evaluated presence-only habitat suitability models for nesting white-headed woodpeckers (Picoides albolarvatus), a narrowly distributed species of conservation concern that occupies dry conifer forests of the Inland Pacific Northwest, USA. We developed 2 models using Mahalanobis D-2 and Maxent techniques from nest location datasets collected on the eastside of the Cascade Mountain Range, Oregon (1 dataset in 1997-2004 and another, sampling a broader spatial extent, in 2010-2011). Consistent with known ecology of white-headed woodpeckers, both HSI models related positively with percent ponderosa pine, moderate levels of canopy cover (approx. 40%), and moderate-to-high levels of heterogeneity in forest structure. Unlike Mahalanobis HSIs, however, Maxent HSIs were consistently and positively related with nest prevalence and positively related with habitat use estimated with independent point count data. Locations with high Maxent HSIs were characterized by canopy openings adjacent to closed canopy forests. The fact that this habitat feature was described by Maxent HSIs but not by Mahalanobis HSIs possibly explains why Maxent HSIs better predicted white-headed woodpecker occurrence. Additionally, we used presence-absence data for model evaluation that sampled a broader spatial extent than nest surveys and therefore allowed us to demonstrate the generality of Maxent HSIs. Additional nest location data collected across a broader portion of the species range would be valuable for further model improvement and evaluation, but until such data are available, we recommend use of Maxent HSIs to guide habitat conservation and restoration efforts in unburned dry forests of Oregon. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Latif, Quresh S.; Saab, Victoria A.] US Forest Serv, Rocky Mt Res Stn, Bozeman, MT 59717 USA. [Mellen-Mclean, Kim] US Forest Serv, Pacific Northwest Reg, Portland, OR 97208 USA. [Dudley, Jonathan G.] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. RP Latif, QS (reprint author), US Forest Serv, Rocky Mt Res Stn, 1648 S Seventh Ave, Bozeman, MT 59717 USA. EM qlatif@fs.fed.us FU United States Forest Service Pacific Northwest Region; Rocky Mountain Research Station; National Fire Plan FX Funding was provided primarily by the United States Forest Service Pacific Northwest Region, with additional support by the Rocky Mountain Research Station and the National Fire Plan. Nest-site data collection was also supported by Oregon Department of Fish and Wildlife, Audubon Society, M. Stern of The Nature Conservancy, and C. Carey of Oregon Department of Fish and Wildlife. A. Kehoe played a key role in overseeing field operations for collection of the nest and regional occupancy data. We are grateful to all field assistants. R. Frenzel provided initial nest location dataset. We thank L.S. Baggett, B. Bird, N. Seavy, E. Merrill, G. Roloff, and 2 anonymous reviewers for thoughtful reviews of earlier drafts. NR 51 TC 2 Z9 2 U1 3 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD FEB PY 2015 VL 79 IS 2 BP 263 EP 273 DI 10.1002/jwmg.842 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA CA0YO UT WOS:000348639700010 ER PT J AU Honda, KL Lamon-Fava, S Matthan, NR Wu, DY Lichtenstein, AH AF Honda, Kaori L. Lamon-Fava, Stefania Matthan, Nirupa R. Wu, Dayong Lichtenstein, Alice H. TI EPA and DHA Exposure Alters the Inflammatory Response but not the Surface Expression of Toll-like Receptor 4 in Macrophages SO LIPIDS LA English DT Article DE Eicosapentaenoic acid; Docosahexaenoic acid; Macrophages; Toll-like receptor 4; Tumor necrosis factor alpha; Interleukin 6 ID SATURATED FATTY-ACIDS; DOCOSAHEXAENOIC ACID; EICOSAPENTAENOIC ACID; SIGNALING PATHWAYS; CYTOKINE PRODUCTION; MURINE MACROPHAGES; THP-1 CELLS; TNF-ALPHA; FISH-OIL; ATHEROSCLEROSIS AB Dietary intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and their respective enrichment in cell membranes have been negatively associated with atherosclerotic lesion development. This effect may be mediated, in part, by dampened inflammatory response of macrophages triggered by toll-like receptor 4 (TLR4) activation. This study investigated the influence of membrane fatty acid profile on TLR4-mediated inflammation in RAW 264.7 macrophages. Cells pretreated with myristic acid (MA), EPA, DHA or vehicle control for 24 h were stimulated with ultra-pure LPS, a specific TLR4 agonist, for 6 or 24 h, corresponding to early and late stages of TNF alpha and IL-6 protein induction. Treatment significantly increased cell membrane MA, EPA, and DHA by 4.5-, 20.6-, and 8.9-fold, respectively. MA significantly increased IL-6 secretion 6 h post-exposure to the fatty acid, but did not change TNF alpha secretion in response to any other treatment condition. EPA and DHA significantly reduced TNF alpha secretion by 36 and 41 %, respectively, in cells stimulated for 24 h but not 6 h. In contrast, EPA and DHA significantly reduced IL-6 secretion at both 6 h (67 and 72 %, respectively) and 24 h (69 and 72 %, respectively). MA or DHA treatment had no significant effect compared to vehicle on factors influencing cellular LPS recognition, including LPS-cell association, and cell surface expression of TLR4, TLR4-MD2 complex, and CD14. These data suggest that membrane fatty acid profiles influence the TLR4-mediated inflammatory response in macrophages, via mechanisms that occur downstream of TLR4 receptor activation. C1 [Honda, Kaori L.; Lamon-Fava, Stefania; Matthan, Nirupa R.; Wu, Dayong; Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM kaori.honda@tufts.edu; stefania.lamon-fava@tufts.edu; nirupa.matthan@tufts.edu; dayong.wu@tufts.edu; alice.lichtenstein@tufts.edu FU NIH [NHLBI-T32-HL069772]; USDA [58-1950-0-0014] FX This work was supported by the NIH Grant: NHLBI-T32-HL069772 (NLS) and the USDA Agreement No. 58-1950-0-0014. NR 48 TC 11 Z9 11 U1 1 U2 13 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD FEB PY 2015 VL 50 IS 2 BP 121 EP 129 DI 10.1007/s11745-014-3971-y PG 9 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA AZ8CO UT WOS:000348442700002 PM 25408476 ER PT J AU Hadsell, DL Hadsell, LA Olea, W Rijnkels, M Creighton, CJ Smyth, I Short, KM Cox, LL Cox, TC AF Hadsell, Darryl L. Hadsell, Louise A. Olea, Walter Rijnkels, Monique Creighton, Chad J. Smyth, Ian Short, Kieran M. Cox, Liza L. Cox, Timothy C. TI In-silico QTL mapping of postpubertal mammary ductal development in the mouse uncovers potential human breast cancer risk loci SO MAMMALIAN GENOME LA English DT Article ID GENOME-WIDE ASSOCIATION; GLAND DEVELOPMENT; BRANCHING MORPHOGENESIS; GENE-EXPRESSION; MICE LACKING; TARGET SITE; STRAINS; TBX3; IDENTIFICATION; MUTATIONS AB Genetic background plays a dominant role in mammary gland development and breast cancer (BrCa). Despite this, the role of genetics is only partially understood. This study used strain-dependent variation in an inbred mouse mapping panel, to identify quantitative trait loci (QTL) underlying structural variation in mammary ductal development, and determined if these QTL correlated with genomic intervals conferring BrCa susceptibility in humans. For about half of the traits, developmental variation among the complete set of strains in this study was greater (P < 0.05) than that of previously studied strains, or strains in current common use for mammary gland biology. Correlations were also detected with previously reported variation in mammary tumor latency and metastasis. In-silico genome-wide association identified 20 mammary development QTL (Mdq). Of these, five were syntenic with previously reported human BrCa loci. The most significant (P = 1 x 10(-11)) association of the study was on MMU6 and contained the genes Plxna4, Plxna4os1, and Chchd3. On MMU5, a QTL was detected (P = 8 x 10(-7)) that was syntenic to a human BrCa locus on h12q24.5 containing the genes Tbx3 and Tbx5. Intersection of linked SNP (r(2) > 0.8) with genomic and epigenomic features, and intersection of candidate genes with gene expression and survival data from human BrCa highlighted several for further study. These results support the conclusion that mammary tumorigenesis and normal ductal development are influenced by common genetic factors and that further studies of genetically diverse mice can improve our understanding of BrCa in humans. C1 [Hadsell, Darryl L.; Hadsell, Louise A.; Olea, Walter] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Hadsell, Darryl L.] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Rijnkels, Monique] Texas A&M Univ, Dept Vet Integrat Biosci, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Creighton, Chad J.] Baylor Coll Med, Div Biostat, Dept Med, Dan L Duncan Canc Ctr, Houston, TX 77030 USA. [Smyth, Ian] Monash Univ, Dept Anat & Dev Biol, Clayton, Vic 3800, Australia. [Smyth, Ian; Short, Kieran M.] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic 3800, Australia. [Cox, Liza L.; Cox, Timothy C.] Univ Washington, Dept Pediat, Sch Med, Seattle, WA 98101 USA. [Cox, Liza L.; Cox, Timothy C.] Seattle Childrens Res Inst, Ctr Dev Biol & Regenerat Med, Seattle, WA 98101 USA. RP Hadsell, DL (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, 1100 Bates St,Suite 10072,Mail Stop BCM 320, Houston, TX 77030 USA. EM dhadsell@bcm.edu RI Short, Kieran/A-2604-2012 OI Short, Kieran/0000-0001-9137-008X FU NICHD [5R21HD059746]; USDA/ARS [6250-51000-052]; NCI [P30 CA125123]; Future Fellowship from the Australian Research Council FX The authors thank Ms. Elizabeth Lessels for her contribution to the initial phenotyping. Thanks also to Dr. Daniel Gatti for providing advice on the association analysis and for providing R scripts used in processing the SNP dataset in this analysis. Thanks also to Mathew LaCourse for technical assistance related to optical projection tomography, to Dr. John Belmont for providing computing resources for the permutation analysis, and to Fengju Chen for technical assistance related to the mining the human tumor gene expression data. Thanks also to Dr. Jeff Rosen for helpful discussion involving the interpretation of the results, and for valuable suggestions on the manuscript. This Project was supported by NICHD Grant Number 5R21HD059746 (Darryl Hadsell), by USDA/ARS Cooperative Agreement No. 6250-51000-052 (Darryl Hadsell), and by NCI Grant Number P30 CA125123 (Chad Creighton). Ian Smyth holds a Future Fellowship from the Australian Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 112 TC 4 Z9 4 U1 0 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0938-8990 EI 1432-1777 J9 MAMM GENOME JI Mamm. Genome PD FEB PY 2015 VL 26 IS 1-2 BP 57 EP 79 DI 10.1007/s00335-014-9551-x PG 23 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA AZ8CS UT WOS:000348443100005 PM 25552398 ER PT J AU Alekel, DL Genschel, U Koehler, KJ Hofmann, H Van Loan, MD Beer, BS Hanson, LN Peterson, CT Kurzer, MS AF Alekel, D. Lee Genschel, Ulrike Koehler, Kenneth J. Hofmann, Heike Van Loan, Marta D. Beer, Bonnie S. Hanson, Laura N. Peterson, Charles T. Kurzer, Mindy S. TI Soy Isoflavones for Reducing Bone Loss Study: effects of a 3-year trial on hormones, adverse events, and endometrial thickness in postmenopausal women SO MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY LA English DT Article DE Soy isoflavone supplements; Safety; Transvaginal ultrasound; 17 beta-Estradiol; Estrone sulfate; Thyroid-stimulating hormone ID PLACEBO-CONTROLLED TRIAL; DOUBLE-BLIND; MENOPAUSAL SYMPTOMS; ALCOHOL-CONSUMPTION; PROTEIN ISOLATE; CANCER-RISK; HOT FLUSHES; US ADULTS; BREAST; NUTRITION AB Objective: This study aims to assess the overall safety and potential endometrium-stimulating effects of soy isoflavone tablets consumed (3 y) by postmenopausal women and to determine endometrial thickness response to treatment among compliant women, taking into account hormone concentrations and other hypothesized modifying factors. Methods: We randomized healthy postmenopausal women (aged 45.8-65.0 y) to placebo control or two doses (80 or 120 mg/d) of soy isoflavones at two sites. We used intent-to-treat analysis (N = 224) and compliant analysis (>95%; N = 208) to assess circulating hormone concentrations, adverse events, and endometrial thickness (via transvaginal ultrasound). Results: Median values for endometrial thickness (mm) declined from baseline through 36 months. Nonparametric analysis of variance for treatment differences among groups showed no differences in absolute (or percentage of change) endometrial thickness (chi(2) P ranged from 0.12 to 0.69) or in circulating hormones at any time point. A greater number of adverse events in the genitourinary system (P = 0.005) were noted in the 80 mg/day group compared with the 120 mg/day group, whereas other systems showed no treatment effects. The model predicting endometrial thickness response (using natural logarithm) to treatment among compliant women across time points was significant (P <= 0.0001), indicating that estrogen exposure (P = 0.0013), plasma 17 beta-estradiol (P = 0.0086), and alcohol intake (P = 0.023) contributed significantly to the response. Neither the 80 mg/day dose (P = 0.57) nor the 120 mg/day dose (P = 0.43) exerted an effect on endometrial thickness across time. Conclusions: Our randomized controlled trial verifies the long-term overall safety of soy isoflavone tablet intake by postmenopausal women who display excellent compliance. We find no evidence of treatment effects on endometrial thickness, adverse events, or circulating hormone concentrations, most notably thyroid function, across a 3-year period. C1 [Alekel, D. Lee] NIH, Bethesda, MD 20892 USA. [Genschel, Ulrike; Koehler, Kenneth J.; Hofmann, Heike] Iowa State Univ, Dept Stat, Ames, IA USA. [Van Loan, Marta D.] Univ Calif Davis, USDA, ARS, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Beer, Bonnie S.] McFarland Clin, Ames, IA USA. [Hanson, Laura N.] Mayo Validat Support Serv, Rochester, MN USA. [Peterson, Charles T.] US FDA, Ctr Devices & Radiol Hlth, White Oak, MD USA. [Kurzer, Mindy S.] Univ Minnesota, Dept Food Sci & Nutr, St Paul, MN USA. RP Alekel, DL (reprint author), NIH, Natl Ctr Complementary & Alternat Med, Suite 401,6707 Democracy Blvd 2, Bethesda, MD 20892 USA. EM Lee.Alekel@nih.gov FU National Institute of Arthritis and Musculoskeletal and Skin Diseases [RO1 AR046922]; Nutrition and Wellness Research Center at Iowa State University; US Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center [5306-51530-006-00D]; Clinical and Translational Science Center, Clinical Research Center, University of California [1M01RR19975-01]; National Center for Medical Research [UL1 RR024146] FX Funding/support: This work was supported mainly by a grant (RO1 AR046922) from the National Institute of Arthritis and Musculoskeletal and Skin Diseases and was also supported by the Nutrition and Wellness Research Center at Iowa State University; US Department of Agriculture-Agricultural Research Service Western Human Nutrition Research Center (5306-51530-006-00D); Clinical and Translational Science Center, Clinical Research Center, University of California (1M01RR19975-01); and National Center for Medical Research (UL1 RR024146). NR 31 TC 14 Z9 14 U1 3 U2 9 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1072-3714 EI 1530-0374 J9 MENOPAUSE JI Menopause-J. N. Am. Menopause Soc. PD FEB PY 2015 VL 22 IS 2 BP 185 EP 197 DI 10.1097/gme.0000000000000280 PG 13 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA CA2UY UT WOS:000348764600011 PM 25003624 ER PT J AU Jiang, S Zong, Y Yue, XY Postman, J Teng, YW Cai, DY AF Jiang, Shuang Zong, Yu Yue, Xiaoyan Postman, Joseph Teng, Yuanwen Cai, Danying TI Prediction of retrotransposons and assessment of genetic variability based on developed retrotransposon-based insertion polymorphism (RBIP) markers in Pyrus L SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Retrotransposon; Pyrus; RBIP marker; Insertion polymorphism; Genetic relationship ID LTR RETROTRANSPOSONS; POPULATION-STRUCTURE; RAPD MARKERS; EAST-ASIA; TRANSPOSABLE ELEMENTS; PHYLOGENETIC UTILITY; MAIZE GENOME; SSR MARKERS; DNA MARKERS; PEAR AB Interspecific hybridization has been considered the major mode of evolution in Pyrus (pear), and thus, the genetic relationships within this genus have not been well documented. Retrotransposons are ubiquitous components of plant genomes and 42.4 % of the pear genome was reported to be long terminal repeat (LTR) retrotransposons, implying that retrotransposons might be significant in the evolution of Pyrus. In this study, 1,836 putative full-length LTR retrotransposons were isolated and 196 retrotransposon-based insertion polymorphism (RBIP) primers were developed, of which 24 pairs to the Ppcr1 subfamily of copia retrotransposons were used to analyze genetic diversity among 110 Pyrus accessions from Eurasia. Our results showed that Ppcr1 replicated many times in the development of cultivated Asian pears. The genetic structure analysis and the unweighted pair group method with arithmetic mean (UPGMA) dendrogram indicated that all accessions could be divided into Oriental and Occidental groups. In Oriental pears, wild pea pears clustered separately into independent groups in accordance with their morphological classifications. Cultivars of P. ussuriensis Maxim, P. pyrifolia Nakai, and P. pyrifolia Chinese white pear were mingled together, which inferred that hybridization events occurred during the development of the cultivated Asian pears. In Occidental pears, two clades were obtained in the UPGMA dendrogram in accordance with their geographical distribution; one contained the European species and the other included species from North Africa and West Asia. New findings in this study will be important to further understand the phylogeny of Pyrus and origins of cultivated pears. C1 [Jiang, Shuang; Zong, Yu; Yue, Xiaoyan; Teng, Yuanwen; Cai, Danying] Zhejiang Univ, Dept Hort, State Agr Minist Key Lab Hort Plant Growth, Hangzhou 310058, Zhejiang, Peoples R China. [Postman, Joseph] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Teng, YW (reprint author), Zhejiang Univ, Dept Hort, State Agr Minist Key Lab Hort Plant Growth, Hangzhou 310058, Zhejiang, Peoples R China. EM ywteng@zju.edu.cn; dycai@zju.edu.cn RI Teng, Yuanwen/A-3515-2015 FU National Natural Science Foundation of China [31201592]; Specialized Research Fund for the Doctoral Program of Higher Education [20110101110091]; Grant for Innovative Research Team of Zhejiang Province of China [2013TD05] FX This work was financed by a Grant from the National Natural Science Foundation of China (No. 31201592), a Specialized Research Fund for the Doctoral Program of Higher Education (20110101110091), and a Grant for Innovative Research Team of Zhejiang Province of China (2013TD05). NR 59 TC 4 Z9 5 U1 1 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 EI 1617-4623 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD FEB PY 2015 VL 290 IS 1 BP 225 EP 237 DI 10.1007/s00438-014-0914-5 PG 13 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA CA5BZ UT WOS:000348923900018 PM 25216935 ER PT J AU Liu, ZH Holmes, DJ Faris, JD Chao, SM Brueggeman, RS Edwards, MC Friesen, TL AF Liu, Zhaohui Holmes, Danielle J. Faris, Justin D. Chao, Shiaoman Brueggeman, Robert S. Edwards, Michael C. Friesen, Timothy L. TI Necrotrophic effector-triggered susceptibility (NETS) underlies the barley-Pyrenophora teres f. teres interaction specific to chromosome 6H SO MOLECULAR PLANT PATHOLOGY LA English DT Article DE barley; Dothidiomycete; electrolyte leakage; necrotrophic effector-triggered susceptibility; net form net blotch; reactive oxygen species (ROS) ID BLOTCH RESISTANCE GENES; QUANTITATIVE TRAIT LOCI; HOST-SELECTIVE TOXINS; DOUBLED-HAPLOID POPULATION; STAGONOSPORA-NODORUM; DISEASE RESISTANCE; TRITICI-REPENTIS; SPOT BLOTCH; PTR TOXA; CONFERRING RESISTANCE AB Barley net form net blotch (NFNB), caused by the necrotrophic fungus Pyrenophora teres f. teres, is a destructive foliar disease in barley-growing regions worldwide. Little is known about the genetic and molecular basis of this pathosystem. Here, we identified a small secreted proteinaceous necrotrophic effector (NE), designated PttNE1, from intercellular wash fluids of the susceptible barley line Hector after inoculation with P.teres f. teres isolate 0-1. Using a barley recombinant inbred line (RIL) population developed from a cross between the sensitive/susceptible line Hector and the insensitive/resistant line NDB 112 (HN population), sensitivity to PttNE1, which we have named SPN1, mapped to a common resistance/susceptibility region on barley chromosome 6H. PttNE1-SPN1 interaction accounted for 31% of the disease variation when the HN population was inoculated with the 0-1 isolate. Strong accumulation of hydrogen peroxide and increased levels of electrolyte leakage were associated with the susceptible reaction, but not the resistant reaction. In addition, the HN RIL population was evaluated for its reactions to 10 geographically diverse P.teres f. teres isolates. Quantitative trait locus (QTL) mapping led to the identification of at least 10 genomic regions associated with disease, with chromosomes 3H and 6H harbouring major QTLs for resistance/susceptibility. SPN1 was associated with all the 6H QTLs, except one. Collectively, this information indicates that the barley-P.teres f. teres pathosystem follows, at least partially, an NE-triggered susceptibility (NETS) model that has been described in other necrotrophic fungal disease systems, especially in the Dothideomycete class of fungi. C1 [Liu, Zhaohui; Brueggeman, Robert S.; Friesen, Timothy L.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Holmes, Danielle J.; Faris, Justin D.; Chao, Shiaoman; Edwards, Michael C.; Friesen, Timothy L.] USDA ARS, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. RP Friesen, TL (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. EM timothy.friesen@ars.usda.gov FU USDA-NIFA-AFRI [2011-68002-30029]; North Dakota Barley Council; USDA-ARS CRIS [5442-22000-048-00D] FX The authors thank Drs Melvin Bolton and Jared LeBoldus for critical review of the manuscript. This research was supported by funding from USDA-NIFA-AFRI grant #2011-68002-30029 (T-CAP), the North Dakota Barley Council and USDA-ARS CRIS Project no. 5442-22000-048-00D. NR 80 TC 11 Z9 11 U1 4 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD FEB PY 2015 VL 16 IS 2 BP 188 EP 200 DI 10.1111/mpp.12172 PG 13 WC Plant Sciences SC Plant Sciences GA CA2MD UT WOS:000348741600007 PM 25040207 ER PT J AU Lugo, MA Reinhart, KO Menoyo, E Crespo, EM Urcelay, C AF Lugo, Mnica A. Reinhart, Kurt O. Menoyo, Eugenia Crespo, Esteban M. Urcelay, Carlos TI Plant functional traits and phylogenetic relatedness explain variation in associations with root fungal endophytes in an extreme arid environment SO MYCORRHIZA LA English DT Article DE Arbuscular mycorrhizal fungi; Dark septate endophytes; Functional traits; Phylogenetic trait conservatism; Phylogenetic trait divergence; Xeric environment ID ARBUSCULAR MYCORRHIZAL FUNGI; DARK-SEPTATE FUNGI; TALLGRASS PRAIRIE; WARM-SEASON; COLONIZATION; RESPONSES; GRASSES; EVOLUTION; SYMBIOSIS; DEPENDENCE AB Since root endophytes may ameliorate drought stress, understanding which plants associate with endophytes is important, especially in arid ecosystems. Here, the root endophytes were characterized of 42 plants from an arid region of Argentina. Colonization by arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSEs) was related to plant functional type (PFT), family, and phylogenetic relatedness. Overall, three main findings were observed. Firstly, only moderate levels of endophyte associations were found across all taxa (e.g., most Poaceae were not colonized by endophytes despite numerous accounts of colonization by AMF and DSEs). We determined 69 % of plant taxa associated with some form of root endophyte but levels were lower than other regional studies. Secondly, comparisons by PFT and phylogeny were often qualitatively similar (e.g., succulents and Portulacineae consistently lacked AMF; variation occurred among terrestrial vs. epiphytic bromeliads) and often differed from comparisons based on plant family. Thirdly, comparisons by plant family often failed to account for important variation either within families (e.g., Bromeliaceae and Poaceae) or trait conservatism among related families (i.e., Rosidae consistently lacked DSEs and Portulacineae lacked AMF). This study indicates the value of comparing numerous taxa based on PFTs and phylogenetic similarity. Overall, the results suggest an uncertain benefit of endophytes in extremely arid environments where plant traits like succulence may obviate the need to establish associations. C1 [Lugo, Mnica A.; Menoyo, Eugenia; Crespo, Esteban M.] Univ Nacl San Luis, IMIBIO CONICET, RA-5700 San Luis, Argentina. [Reinhart, Kurt O.] ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Urcelay, Carlos] Univ Nacl Cordoba, IMBIV CONICET, RA-5000 Cordoba, Argentina. RP Lugo, MA (reprint author), Univ Nacl San Luis, IMIBIO CONICET, RA-5700 San Luis, Argentina. EM monicalugo63@gmail.com FU [PROICO 2-O203]; [PICT 0781-2008] FX We are grateful to Brian Anacker for comments on the manuscript. This work was supported by grants from PROICO 2-O203, and PICT 0781-2008. C.U. wish to acknowledge Secyt, Mincyt (Cordoba), and the assistance of the Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) and the Universidad Nacional de Cordoba, both of which have provided facilities used for this study. M.L., C.U. are researchers from Argentine Council (CONICET) and K.R. from Agricultural Research Service (USDA). The authors declare that they have no conflict of interest. NR 57 TC 4 Z9 4 U1 12 U2 52 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0940-6360 EI 1432-1890 J9 MYCORRHIZA JI Mycorrhiza PD FEB PY 2015 VL 25 IS 2 BP 85 EP 95 DI 10.1007/s00572-014-0592-5 PG 11 WC Mycology SC Mycology GA AZ8FO UT WOS:000348450600001 PM 24997550 ER PT J AU Poznanovic, SK Lilleskov, EA Webster, CR AF Poznanovic, Sarah K. Lilleskov, Erik A. Webster, Christopher R. TI Sharing rotting wood in the shade: ectomycorrhizal communities of co-occurringbirch and hemlock seedlings SO MYCORRHIZA LA English DT Article DE Ectomycorrhizal fungi; Seedlings; Coarsewoody debris; Foliar nutrients; Multivariate analysis; Tsuga canadensis; Betula alleghaniensis ID COMMON MYCORRHIZAL NETWORKS; FUNGAL COMMUNITIES; TSUGA-CANADENSIS; EASTERN HEMLOCK; CANOPY TREES; PICEA-ABIES; FORESTS; PLANTS; GROWTH; ASSOCIATIONS AB Coarse woody debris (CWD)is an important nursery environment for many tree species. Understanding the communities of ectomycorrhizal fungi (ECMF)and the effect of ECMF species on tree seedling condition in CWD will elucidate the potential for ECMF-mediatedeffects on seedling dynamics. In hemlock-dominatedstands, we characterized ECMF communities associated with eastern hemlock (Tsuga canadensis (L.) Carr) and yellow birch (Betula alleghaniensis Britt) seedling pairs growing on CWD. Seedling foliage and CWD were analyzed chemically, and seedling growth, canopy cover, and canopy species determined. Thirteen fungal taxa, 12 associated with birch, and 6 with hemlock, were identified based on morphology and ITS sequencing. Five species were shared by co-occurringbirch and hemlock, representing 75 % of ectomycorrhizal root tips. Rarified ECMF taxon richness per seedling was higher on birch than hemlock. Nonmetric multidimensional scaling revealed significant correlations between ordination axes, the mutually exclusive ECMF Tomentella and Lactarius spp., foliar N and K, CWD pH, and exchangeable Ca and Mg. Seedlings colonized by Lactarius and T. sublilacina differed significantly in foliar K and N, and CWD differed in exchangeable Ca and Mg. CWD pH and nutrient concentrations were low but foliar macro-nutrientconcentrations were not. We hypothesize that the dominant ECMF are adapted to low root carbohydrate availability typical in shaded environments but differ in their relative supply of different nutrients. C1 [Poznanovic, Sarah K.; Webster, Christopher R.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Lilleskov, Erik A.] US Forest Serv, Forestry Sci Lab, No Res Stn, USDA, Houghton, MI 49931 USA. RP Lilleskov, EA (reprint author), US Forest Serv, Forestry Sci Lab, No Res Stn, USDA, 410 MacInnes Dr, Houghton, MI 49931 USA. EM elilleskov@fs.fed.us FU McIntire-Stennis Cooperative Forestry Research Program; Ecosystem Science Center at Michigan Technological University; USDA Forest Service, Northern Research Station, Houghton, MI FX Funding for this project was provided by the McIntire-Stennis Cooperative Forestry Research Program, the Ecosystem Science Center at Michigan Technological University, and by the USDA Forest Service, Northern Research Station, Houghton, MI. We would especially like to thank Louis J. Lamit for help with statistical analysis and Lynette Potvin for help with laboratory techniques and methods. We would also like to thank two anonymous reviewers for commenting on earlier drafts of this manuscript. NR 70 TC 2 Z9 3 U1 6 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0940-6360 EI 1432-1890 J9 MYCORRHIZA JI Mycorrhiza PD FEB PY 2015 VL 25 IS 2 BP 153 EP 164 DI 10.1007/s00572-014-0597-0 PG 12 WC Mycology SC Mycology GA AZ8FO UT WOS:000348450600007 PM 25091153 ER PT J AU Balint-Kurti, PJ Holland, JB AF Balint-Kurti, Peter J. Holland, James B. TI New insight into a complex plant-fungal pathogen interaction SO NATURE GENETICS LA English DT Editorial Material ID MAIZE AB The coevolution of plants and microbes has shaped plant mechanisms that detect and repel pathogens. A newly identified plant gene confers partial resistance to a fungal pathogen not by preventing initial infection but by limiting its spread through the plant. C1 [Balint-Kurti, Peter J.; Holland, James B.] N Carolina State Univ, USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Balint-Kurti, Peter J.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Balint-Kurti, PJ (reprint author), N Carolina State Univ, USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM peter_alintkurti@ncsu.edu OI Balint-Kurti, Peter/0000-0002-3916-194X NR 13 TC 3 Z9 3 U1 4 U2 48 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD FEB PY 2015 VL 47 IS 2 BP 101 EP 103 PG 3 WC Genetics & Heredity SC Genetics & Heredity GA CA1TQ UT WOS:000348694700003 PM 25627898 ER PT J AU Ryan, MG Sapes, G Sala, A Hood, SM AF Ryan, Michael G. Sapes, Gerard Sala, Anna Hood, Sharon M. TI Tree physiology and bark beetles SO NEW PHYTOLOGIST LA English DT Editorial Material DE drought; induced defense; irruptive bark beetles; resin production; tree mortality ID NATURAL DISTURBANCES; DYNAMICS; DROUGHT; PINE; MORTALITY; FEEDBACK; BEHAVIOR; ATTACKS; FORESTS C1 [Ryan, Michael G.] Colorado State Univ, Nat Resources Ecol Lab, Ft Collins, CO 80523 USA. [Ryan, Michael G.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80521 USA. [Sapes, Gerard; Sala, Anna; Hood, Sharon M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Ryan, MG (reprint author), Colorado State Univ, Nat Resources Ecol Lab, Ft Collins, CO 80523 USA. EM Mike.Ryan@colostate.edu RI Hood, Sharon/E-5209-2015; Ryan, Michael/A-9805-2008 OI Hood, Sharon/0000-0002-9544-8208; Ryan, Michael/0000-0002-2500-6738 NR 14 TC 0 Z9 0 U1 2 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD FEB PY 2015 VL 205 IS 3 BP 955 EP 957 DI 10.1111/nph.13256 PG 3 WC Plant Sciences SC Plant Sciences GA CA2HX UT WOS:000348730600003 PM 25580650 ER PT J AU Choat, B Brodersen, CR McElrone, AJ AF Choat, Brendan Brodersen, Craig R. McElrone, Andrew J. TI Synchrotron X-ray microtomography of xylem embolism in Sequoia sempervirens saplings during cycles of drought and recovery SO NEW PHYTOLOGIST LA English DT Article DE cavitation; embolism; microCT; redwood; refilling; Sequoia; xylem ID STRESS-INDUCED CAVITATION; BORDERED PIT STRUCTURE; RESOLUTION COMPUTED-TOMOGRAPHY; IN-VIVO VISUALIZATIONS; LAURUS-NOBILIS; HYDRAULIC EFFICIENCY; NEGATIVE PRESSURES; VITIS-VINIFERA; WATER-DEFICIT; D. DON AB The formation of emboli in xylem conduits can dramatically reduce hydraulic capacity and represents one of the principal mechanisms of drought-induced mortality in woody plants. However, our understanding of embolism formation and repair is constrained by a lack of tools to directly and nondestructively measure these processes at high spatial resolution. Using synchrotron-based microcomputed tomography (microCT), we examined embolism in the xylem of coast redwood (Sequoia sempervirens) saplings that were subjected to cycles of drought and rewatering. Embolism formation was observed occurring by three different mechanisms: as tracheids embolizing in wide tangential bands; as isolated tracheids in seemingly random events; and as functional groups connected to photosynthetic organs. Upon rewatering, stem water potential recovered to predrought stress levels within 24h; however, no evidence of embolism repair was observed even after a further 2wk under well-watered conditions. The results indicate that intertracheid air seeding is the primary mechanism by which embolism spreads in the xylem of S.sempervirens, but also show that a small number of tracheids initially become gas-filled via another mechanism. The inability of S.sempervirens saplings to reverse drought-induced embolism is likely to have important ecological impacts on this species. C1 [Choat, Brendan] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. [Brodersen, Craig R.] Yale Univ, Sch Forestry Environm Studies, New Haven, CT 06511 USA. [McElrone, Andrew J.] ARS, USDA, Davis, CA 95616 USA. [McElrone, Andrew J.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. RP Choat, B (reprint author), Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. EM b.choat@uws.edu.au RI Brodersen, Craig/J-1112-2016 OI Brodersen, Craig/0000-0002-0924-2570 FU National Science Foundation [0818479]; US Department of Agriculture-Agricultural Research Service Current Research Information System funding [5306-21220-004-00]; Office of Science of the US Department of Energy [DE-AC02-05CH11231]; Office of Basic Energy Sciences, of the US Department of Energy [DE-AC02-05CH11231]; International Synchrotron Access Program (ISAP) FX We thank D. Parkinson and A. MacDowell for their assistance at the Lawrence Berkeley National Laboratory ALS beamline 8.3.2 microtomography facility. Danielle Creek is thanked for assistance with light microscopy, and Amr Zedan and Zainab Nasafi for assistance with image analysis. This work was supported by the National Science Foundation (grant no. 0818479) and US Department of Agriculture-Agricultural Research Service Current Research Information System funding (research project no. 5306-21220-004-00). The ALS is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the US Department of Energy (contract no. DE-AC02-05CH11231). B.C. acknowledges travel funding from the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron. The ISAP is an initiative of the Australian government being conducted as part of the National Collaborative Research Infrastructure Strategy. NR 79 TC 22 Z9 22 U1 5 U2 45 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD FEB PY 2015 VL 205 IS 3 BP 1095 EP 1105 DI 10.1111/nph.13110 PG 11 WC Plant Sciences SC Plant Sciences GA CA2HX UT WOS:000348730600022 PM 25385085 ER PT J AU Moreno, JP Johnston, CA Chen, TA O'Connor, TA Hughes, SO Baranowski, J Woehler, D Baranowski, T AF Moreno, Jennette P. Johnston, Craig A. Chen, Tzu-An O'Connor, Teresia A. Hughes, Sheryl O. Baranowski, Janice Woehler, Deborah Baranowski, Tom TI Seasonal Variability in Weight Change During Elementary School SO OBESITY LA English DT Article ID BODY-MASS INDEX; INNER-CITY NEIGHBORHOODS; SUMMER VACATION; MIDDLE SCHOOL; UNITED-STATES; LOW-INCOME; OBESITY; CHILDREN; OVERWEIGHT; CHILDHOOD AB ObjectiveTo examine seasonal variation in weight gain across elementary school (kindergarten-5th grade) among children who are healthy weight, overweight, or obese and from different racial and ethnic groups. MethodsThe sample included 7,599 ethnically diverse students ages 5-7 years at baseline (Caucasian: 21.1%, Black: 36.2%, Hispanic: 26.0%, Asian 16.7%). Heights and weights were measured by school nurses at the beginning and end of each school year from kindergarten through the beginning of 5th grade. ResultsBeginning the summer after 1st grade, all children demonstrated a pattern of standardized BMI (zBMI) increases during the summer (0.04 to 0.09) and zBMI decreases across the school years (-0.06 to 0.00; P<0.0001). Hispanic children and children who were overweight and obese exhibited this pattern in kindergarten while children of other ethnicities and with a healthy weight did not (P<0.0001). ConclusionsBeginning the summer after 1st grade, a consistent pattern of relative weight gain during the summer months ( in BMI percentile=1.04) and weight loss during the school year ( in BMI percentile=-0.34) emerged. This pattern appeared earlier for children who were overweight, obese, or Hispanic. These findings suggest a need to better understand the causes of the problematic increases in weight during the summer. C1 [Moreno, Jennette P.; Johnston, Craig A.; Chen, Tzu-An; O'Connor, Teresia A.; Hughes, Sheryl O.; Baranowski, Janice; Baranowski, Tom] Baylor Coll Med, Dept Pediat Nutr, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Johnston, Craig A.] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. [Woehler, Deborah] Oliver Fdn, Houston, TX USA. RP Moreno, JP (reprint author), Baylor Coll Med, Dept Pediat Nutr, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM palcic@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU Oliver Foundation; USDA/ARS [58-6250-6001] FX The authors would like to thank The Oliver Foundation for its support. This work also has had been funded, in part, with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-6001. NR 39 TC 6 Z9 6 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD FEB PY 2015 VL 23 IS 2 BP 422 EP 428 DI 10.1002/oby.20977 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA CA6QN UT WOS:000349040400026 PM 25557689 ER PT J AU Mattingly, WB Orrock, JL Collins, CD Brudvig, LA Damschen, EI Veldman, JW Walker, JL AF Mattingly, W. Brett Orrock, John L. Collins, Cathy D. Brudvig, Lars A. Damschen, Ellen I. Veldman, Joseph W. Walker, Joan L. TI Historical agriculture alters the effects of fire on understory plant beta diversity SO OECOLOGIA LA English DT Article DE Agriculture; Beta diversity; Fire; Land-use legacies; Longleaf pine ID PAST LAND-USE; LONGLEAF PINE WOODLANDS; BIOTIC HOMOGENIZATION; SPECIES-DIVERSITY; FOREST; BIODIVERSITY; PATTERNS; COMMUNITIES; ECOSYSTEM; VEGETATION AB Land-use legacies are known to shape the diversity and distribution of plant communities, but we lack an understanding of whether historical land use influences community responses to contemporary disturbances. Because human-modified landscapes often bear a history of multiple land-use activities, this contingency can challenge our understanding of land-use impacts on plant diversity. We address this contingency by evaluating how beta diversity (the spatial variability of species composition), an important component of regional biodiversity, is shaped by interactions between historical agriculture and prescribed fire, two prominent disturbances that are often coincident in terrestrial ecosystems. At three study locations spanning 450 km in the southeastern United States, we surveyed longleaf pine woodland understory plant communities across 232 remnant and post-agricultural sites with differing prescribed fire regimes. Our results demonstrate that agricultural legacies are a strong predictor of beta diversity, but the direction of this land-use effect differed among the three study locations. Further, although beta diversity increased with prescribed fire frequency at each study location, this effect was influenced by agricultural land-use history, such that positive fire effects were only documented among sites that lacked a history of agriculture at two of our three study locations. Our study not only highlights the role of historical agriculture in shaping beta diversity in a fire-maintained ecosystem but also illustrates how this effect can be contingent upon fire regime and geographic location. We suggest that interactions among historical and contemporary land-use activities may help to explain dissimilarities in plant communities among sites in human-dominated landscapes. C1 [Mattingly, W. Brett; Orrock, John L.; Damschen, Ellen I.; Veldman, Joseph W.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Collins, Cathy D.] Colby Coll, Dept Biol, Waterville, ME 04901 USA. [Brudvig, Lars A.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Walker, Joan L.] US Forest Serv, Southern Res Stn, Clemson, SC 29634 USA. RP Mattingly, WB (reprint author), Eastern Connecticut State Univ, Dept Biol, Willimantic, CT 06226 USA. EM mattinglyw@easternct.edu FU Strategic Environmental Research and Development Program [RC-1695]; Department of Agriculture, Forest Service, Savannah River, under Department of Energy, Aiken, SC [DE-AI09-00SR22188] FX This study was funded by the Strategic Environmental Research and Development Program (Project RC-1695) and by funds provided to the Department of Agriculture, Forest Service, Savannah River, under Interagency Agreement DE-AI09-00SR22188 with the Department of Energy, Aiken, SC. For assistance at field locations, we thank J. Gray and D. Heins (Ft. Bragg); T. Beatty and D. Lavender (Ft. Stewart); and J. Blake, K. Lawrence, and E. Olson (SRS). For assistance with data collection, we thank L. Bizzari, C. Christopher, A. Powell, and R. Ranalli. E. Grman provided helpful comments on previous drafts of this manuscript. This study complied with the current laws of the United States. NR 69 TC 2 Z9 2 U1 4 U2 49 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD FEB PY 2015 VL 177 IS 2 BP 507 EP 518 DI 10.1007/s00442-014-3144-y PG 12 WC Ecology SC Environmental Sciences & Ecology GA CA5XK UT WOS:000348981500019 PM 25411111 ER PT J AU Gornish, ES Aanderud, ZT Sheley, RL Rinella, MJ Svejcar, T Englund, SD James, JJ AF Gornish, Elise S. Aanderud, Zachary T. Sheley, Roger L. Rinella, Mathew J. Svejcar, Tony Englund, Suzanne D. James, Jeremy J. TI Altered snowfall and soil disturbance influence the early life stage transitions and recruitment of a native and invasive grass in a cold desert SO OECOLOGIA LA English DT Article DE Climate change; Establishment; Freeze-thaw cycles; Rangeland; Seedling survival ID BROMUS-TECTORUM; SEED-GERMINATION; ESTABLISHMENT SUCCESS; CLIMATE-CHANGE; GROWTH; SEEDLINGS; IMPACT; VEGETATION; DYNAMICS; TUNDRA AB Climate change effects on plants are expected to be primarily mediated through early life stage transitions. Snowfall variability, in particular, may have profound impacts on seedling recruitment, structuring plant populations and communities, especially in mid-latitude systems. These water-limited and frequently invaded environments experience tremendous variation in snowfall, and species in these systems must contend with harsh winter conditions and frequent disturbance. In this study, we examined the mechanisms driving the effects of snowpack depth and soil disturbance on the germination, emergence, and establishment of the native Pseudoroegnaria spicata and the invasive Bromus tectorum, two grass species that are widely distributed across the cold deserts of North America. The absence of snow in winter exposed seeds to an increased frequency and intensity of freeze-thaw cycles and greater fungal pathogen infection. A shallower snowpack promoted the formation of a frozen surface crust, reducing the emergence of both species (more so for P. spicata). Conversely, a deeper snowpack recharged the soil and improved seedling establishment of both species by creating higher and more stable levels of soil moisture availability following spring thaw. Across several snow treatments, experimental disturbance served to decrease the cumulative survival of both species. Furthermore, we observed that, regardless of snowpack treatment, most seed mortality (70-80 %) occurred between seed germination and seedling emergence (November-March), suggesting that other wintertime factors or just winter conditions in general limited survival. Our results suggest that snowpack variation and legacy effects of the snowpack influence emergence and establishment but might not facilitate invasion of cold deserts. C1 [Gornish, Elise S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Aanderud, Zachary T.; Englund, Suzanne D.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Sheley, Roger L.; Svejcar, Tony; James, Jeremy J.] ARS, USDA, Burns, OR 97720 USA. [Rinella, Mathew J.] ARS, USDA, Miles City, MT 59301 USA. [James, Jeremy J.] Univ Calif, Sierra Foothills Res & Extens Ctr, Div Agr & Nat Resources, Browns Valley, CA 95918 USA. RP Gornish, ES (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM egornish@ucdavis.edu FU NIFA [2011-38415-31158] FX We appreciate the hard work of Brett Bingham and Lori Ziegenhagen for field assistance. This research was funded by NIFA award # 2011-38415-31158. The experiments described in this paper comply with the current laws of the country (USA) in which this work was performed. NR 62 TC 9 Z9 9 U1 8 U2 65 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD FEB PY 2015 VL 177 IS 2 BP 595 EP 606 DI 10.1007/s00442-014-3180-7 PG 12 WC Ecology SC Environmental Sciences & Ecology GA CA5XK UT WOS:000348981500026 PM 25539620 ER PT J AU Abdelraheem, A Hughs, SE Jones, DC Zhang, JF AF Abdelraheem, Abdelraheem Hughs, Sidney E. Jones, Don C. Zhang, Jinfa TI Genetic analysis and quantitative trait locus mapping of PEG-induced osmotic stress tolerance in cotton SO PLANT BREEDING LA English DT Article DE cotton; backcross inbred line; osmotic tolerance; QTL mapping; polyethylene glycol ID X GOSSYPIUM-BARBADENSE; INBRED LINE POPULATION; CULTIVATED TETRAPLOID COTTON; MARKER-ASSISTED SELECTION; FIBER QUALITY; UPLAND COTTON; DROUGHT TOLERANCE; QTL ANALYSIS; PHYSIOLOGICAL-RESPONSES; YIELD COMPONENTS AB Water stress is one of the major abiotic stresses that adversely affect cotton production. Seedlings of 142 backcross inbred lines (BILs) derived from Pima cotton Pima S-7' (Gossypium barbadense L.)xUpland cotton Sure-Grow 747'(G.hirsutum L.) were evaluated in two tests for plant height, fresh shoot weight and root weight under two treatments (5% PEG and water-control conditions) using a hydroponic system in the greenhouse. The experiment in each test was a randomized complete block design with three replicates. The analysis of variance for the two tests detected significant genotypic variation in PEG-induced stress tolerance within the BIL population and between the parents. Heritabilities were moderate to high and were higher under the control conditions than under the PEG treatment, and the three traits were also significantly and positively correlated. Based on a linkage map with 292 loci, six QTLs were detected including two for plant height, and two each for fresh shoot weight and root weight. This study represents the first report in using a permanent mapping population in genetic and linkage analysis of water stress tolerance in cotton. C1 [Abdelraheem, Abdelraheem; Zhang, Jinfa] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Hughs, Sidney E.] USDA ARS, Southwestern Cotton Ginning Res Lab, Mesilla Pk, NM 88047 USA. [Jones, Don C.] Cotton Inc, Cary, NC 27513 USA. RP Zhang, JF (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM jinzhang@nmsu.edu NR 73 TC 1 Z9 1 U1 6 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD FEB PY 2015 VL 134 IS 1 BP 111 EP 120 DI 10.1111/pbr.12228 PG 10 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA CA4BW UT WOS:000348850200014 ER PT J AU Palla, KJ Pijut, PM AF Palla, Kaitlin J. Pijut, Paula M. TI Agrobacterium-mediated genetic transformation of Fraxinus americana hypocotyls SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Fraxinus; Genetic transformation; Organogenesis; Regeneration; White ash ID EMERALD ASH BORER; PLANT-REGENERATION; INVASION; CULTURES; TOBACCO; PRUNUS; L. AB An Agrobacterium tumefaciens-mediated genetic transformation system was successfully developed for white ash (Fraxinus americana) using hypocotyls as the initial explants. Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 A mu M 6-benzyladenine (BA) and 0.5 A mu M thidiazuron (TDZ) were transformed using A. tumefaciens strain EHA105 harboring the binary vector pq35GR containing a fusion gene between neomycin phosphotransferase (nptII) and gusA, as well as an enhanced green fluorescent protein (EGFP). Explants were transformed in a bacterial suspension with 100 A mu M acetosyringone using 90 s sonication and 10 min vacuum infiltration. Putative transformed shoots representing seven independent lines were selectively regenerated on MS medium with 22.2 A mu M BA, 0.5 A mu M TDZ, 50 mg L-1 adenine sulfate, 10 % coconut water, 30 mg L-1 kanamycin, and 500 mg L-1 timentin. Timentin at 500 mg L-1 was optimal for controlling excess bacterial growth, and transformed shoots were selected using 30 mg L-1 kanamycin. The presence of GUS (beta-glucuronidase), nptII, and EGFP in transformed plants was confirmed by polymerase chain reaction (PCR). Reverse transcription-PCR and fluorescence microscopy confirmed the expression of EGFP. Transgenic microshoots were rooted (80 %) on woody plant medium supplemented with 4.9 A mu M indole-3-butyric acid, 2.9 A mu M indole-3-acetic acid, and 500 mg L-1 timentin, and subsequently acclimatized to the culture room. This transformation protocol provides the framework for future genetic modification of white ash to produce plant material resistant to the emerald ash borer. C1 [Palla, Kaitlin J.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. RP Pijut, PM (reprint author), US Forest Serv, USDA, No Res Stn, HTIRC, 715 West State St, W Lafayette, IN 47907 USA. EM ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 FU Fred M. van Eck scholarship for Purdue University; U.S. Department of Agriculture-Animal and Plant Health Inspection Service-Plant Protection and Quarantine-Center for Plant Health Science and Technology FX The authors gratefully acknowledge Drs. Zong-Ming (Max) Cheng, Manjul Dutt, and Vibha Srivastava for their constructive review and suggestions for the improvement of this manuscript. The authors would also like to acknowledge Dr. Jody Banks (Purdue University) for use of the fluorescence stereomicroscope and Dr. Dennis J. Gray (University of Florida) for the transformation vector pq35GR. This research was supported by a Fred M. van Eck scholarship for Purdue University to Kaitlin J. Palla, with partial funding from the U.S. Department of Agriculture-Animal and Plant Health Inspection Service-Plant Protection and Quarantine-Center for Plant Health Science and Technology. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 36 TC 2 Z9 3 U1 3 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD FEB PY 2015 VL 120 IS 2 BP 631 EP 641 DI 10.1007/s11240-014-0630-1 PG 11 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA CA3LE UT WOS:000348807200019 ER PT J AU Shafer, ABA Wolf, JBW Alves, PC Bergstrom, L Bruford, MW Brannstrom, I Colling, G Dalen, L De Meester, L Ekblom, R Fawcett, KD Fior, S Hajibabaei, M Hill, JA Hoezel, AR Hoglund, J Jensen, EL Krause, J Kristensen, TN Krutzen, M McKay, JK Norman, AJ Ogden, R Ouml;sterling, EM Ouborg, NJ Piccolo, J Popovic, D Primmer, CR Reed, FA Roumet, M Salmona, J Schenekar, T Schwartz, MK Segelbacher, G Senn, H Thaulow, J Valtonen, M Veale, A Vergeer, P Vijay, N Vila, C Weissensteiner, M Wennerstrom, L Wheat, CW Zielinski, P AF Shafer, Aaron B. A. Wolf, Jochen B. W. Alves, Paulo C. Bergstrom, Linnea Bruford, Michael W. Brannstrom, Ioana Colling, Guy Dalen, Love De Meester, Luc Ekblom, Robert Fawcett, Katie D. Fior, Simone Hajibabaei, Mehrdad Hill, Jason A. Hoezel, A. Rus Hoglund, Jacob Jensen, Evelyn L. Krause, Johannes Kristensen, Torsten N. Kruetzen, Michael McKay, John K. Norman, Anita J. Ogden, Rob Osterling, E. Martin Ouborg, N. Joop Piccolo, John Popovic, Danijela Primmer, Craig R. Reed, Floyd A. Roumet, Marie Salmona, Jordi Schenekar, Tamara Schwartz, Michael K. Segelbacher, Gernot Senn, Helen Thaulow, Jens Valtonen, Mia Veale, Andrew Vergeer, Philippine Vijay, Nagarjun Vila, Caries Weissensteiner, Matthias Wennerstrom, Lovisa Wheat, Christopher W. Zielinski, Piotr TI Genomics and the challenging translation into conservation practice SO TRENDS IN ECOLOGY & EVOLUTION LA English DT Article ID GENETIC DIVERSITY; BACKGROUND SELECTION; POPULATION GENOMICS; INSULAR POPULATION; DNA; DIVERGENCE; SPECIATION; EVOLUTION; SEQUENCE; MARKERS AB The global loss of biodiversity continues at an alarming rate. Genomic approaches have been suggested as a promising tool for conservation practice as scaling up to genome-wide data can improve traditional conservation genetic inferences and provide qualitatively novel insights. However, the generation of genomic data and subsequent analyses and interpretations remain challenging and largely confined to academic research in ecology and evolution. This generates a gap between basic research and applicable solutions for conservation managers faced with multifaceted problems. Before the real-world conservation potential of genomic research can be realized, we suggest that current infrastructures need to be modified, methods must mature, analytical pipelines need to be developed, and successful case studies must be disseminated to practitioners. C1 [Shafer, Aaron B. A.; Wolf, Jochen B. W.; Bergstrom, Linnea; Brannstrom, Ioana; Ekblom, Robert; Hoglund, Jacob; Vijay, Nagarjun; Weissensteiner, Matthias] Uppsala Univ, Ecol & Genet Evolutionary Biol Ctr, S-75236 Uppsala, Sweden. [Alves, Paulo C.] Univ Porto, Fac Ciencias, CIBIO InBIO, P-4485661 Oporto, Portugal. [Bruford, Michael W.] Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales. [Colling, Guy] Musee Natl Hist Nat Luxembourg, L-2160 Luxembourg, Luxembourg. [Dalen, Love] Swedish Museum Nat Hist, S-10405 Stockholm, Sweden. [De Meester, Luc] Katholieke Univ Leuven, B-3000 Louvain, Belgium. [Fawcett, Katie D.] Univ Groningen, Behav Ecol & Selforg, NL-9712 Groningen, Netherlands. [Fior, Simone; Roumet, Marie] ETH, CH-8092 Zurich, Switzerland. [Hajibabaei, Mehrdad] Univ Guelph, Guelph, ON N1G 2W1, Canada. [Hill, Jason A.; Wennerstrom, Lovisa; Wheat, Christopher W.] Univ Stockholm, S-10691 Stockholm, Sweden. [Hoezel, A. Rus] Univ Durham, Durham DH1 3LE, England. [Jensen, Evelyn L.; Veale, Andrew] Univ British Columbia Okanagan, Kelowna, BC V1V 1V7, Canada. [Krause, Johannes] Univ Tubingen, D-72070 Tubingen, Germany. [Kristensen, Torsten N.] Aalborg Univ, DK-9220 Aalborg, Denmark. [Kruetzen, Michael] Univ Zurich, Anthropol Inst & Museum, CH-8057 Zurich, Switzerland. [McKay, John K.] Colorado State Univ, Ft Collins, CO 80523 USA. [Norman, Anita J.] Swedish Univ Agr Sci, S-90183 Umea, Sweden. [Ogden, Rob; Senn, Helen] Royal Zool Soc Scotland, WildGenes Lab, Edinburgh EH12 6TS, Midlothian, Scotland. [Osterling, E. Martin; Piccolo, John] Karlstad Univ, S-65188 Karlstad, Sweden. [Ouborg, N. Joop] Radboud Univ Nijmegan, NL-6500 GL Nijmegen, Netherlands. [Popovic, Danijela] Univ Warsaw, Ctr New Technol, PL-00681 Warsaw, Poland. [Primmer, Craig R.] Univ Turku, Turku 20014, Finland. [Reed, Floyd A.] Univ Hawaii Manoa, Honolulu, HI 96822 USA. [Salmona, Jordi] Inst Gulbenkian Ciencias, Populat & Conservat Genet Grp, P-2780156 Oeiras, Portugal. [Schenekar, Tamara] Karl Franzens Univ Graz, A-8010 Graz, Austria. [Schwartz, Michael K.] USDA, Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. [Segelbacher, Gernot] Univ Freiburg, D-79106 Freiburg, Germany. [Thaulow, Jens] Norwegian Inst Water Res, N-0349 Oslo, Norway. [Valtonen, Mia] Univ Eastern Finland, Joensuu 80101, Finland. [Vergeer, Philippine] Wageningen Univ, NL-6708 PB Wageningen, Netherlands. [Vila, Caries] Conservat & Evolutionary Genet Grp, Estac Biol Donana, Almonte 41092, Spain. [Zielinski, Piotr] Jagiellonian Univ, Inst Environm Sci, PL-30387 Krakow, Poland. RP Shafer, ABA (reprint author), Uppsala Univ, Ecol & Genet Evolutionary Biol Ctr, S-75236 Uppsala, Sweden. EM aaron.shafer@ebc.uu.se; jochen.wolf@ebc.uu.se RI Bruns, Hella/K-1758-2014; Krause, Johannes/E-6640-2015; Ouborg, Joop/C-1028-2011; Dalen, Love/A-6686-2008; ALVES, Paulo Celio/B-5448-2009; Schwartz, Michael/C-3184-2014; Bruford, Michael/D-3750-2009; McKay, John/K-3875-2012; Vila, Carles/H-4893-2013; CSIC, EBD Donana/C-4157-2011; Primmer, Craig/B-8179-2008 OI Hoglund, Jacob/0000-0002-5840-779X; Krutzen, Michael/0000-0003-1055-5299; De Meester, Luc/0000-0001-5433-6843; Norman, Anita/0000-0002-9499-758X; Bruns, Hella/0000-0001-5752-2254; Krause, Johannes/0000-0001-5475-4690; Dalen, Love/0000-0001-8270-7613; ALVES, Paulo Celio/0000-0003-4797-0939; Schwartz, Michael/0000-0003-3521-3367; Bruford, Michael/0000-0001-6357-6080; McKay, John/0000-0003-4311-5513; Vila, Carles/0000-0002-4206-5246; CSIC, EBD Donana/0000-0003-4318-6602; Primmer, Craig/0000-0002-3687-8435 FU ConGenOmics Initiative of the European Science Foundation [5005]; Swedish Research Council [70720201]; Uppsala University FX This work arose from the workshop 'Conservation genomics: academic exercise or transition with real-world implications?' organized by A.B.A.S. and J.B.W.W. and held at Wik's Castle, Uppsala, Sweden on 18-21 March 2014. The workshop was funded by the ConGenOmics Initiative of the European Science Foundation (Ref. no 5005), the Swedish Research Council (ID 70720201), and Uppsala University. The workshop program is included in the supplementary material online (Appendix Si). All authors contributed to the discussions and participated in drafting the manuscript. The final version of the manuscript benefited from comments by Paul Craze, Paul Hohenlohe, and an anonymous reviewer. NR 90 TC 61 Z9 61 U1 40 U2 223 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0169-5347 J9 TRENDS ECOL EVOL JI Trends Ecol. Evol. PD FEB PY 2015 VL 30 IS 2 BP 78 EP 87 DI 10.1016/j.tree.2014.11.009 PG 10 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA CA9UQ UT WOS:000349270700003 PM 25534246 ER PT J AU Sarvary, MA Cooperband, MF Hajek, AE AF Sarvary, Mark A. Cooperband, Miriam F. Hajek, Ann E. TI The importance of olfactory and visual cues in developing better monitoring tools for Sirex noctilio (Hymenoptera: Siricidae) SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Insect detection; intercept trap; kairomones; olfactory cues; pheromones; phototaxis; visual cues ID EMERALD ASH BORER; PINUS-RADIATA; CARBON-DIOXIDE; EUROPEAN WOODWASP; NORTH-AMERICA; TRAP TREES; PATHOGENICITY; MECHANISM; VOLATILES; MOSQUITOS AB To improve the monitoring of the invasive European woodwasp, both sexes of Sirex noctilio were studied in a walk-in wind tunnel. We evaluated three trap types: unbaited traps, traps baited with a three-component pheromone lure and traps baited with a commercial Sirex kairomone lure and ultraviolet light. When no lure was present, the black intercept trap caught more females than the clear jar trap. The increase in pheromone concentration from 0.1 to 1 mg increased the capture of females, and not males, in the black intercept panel trap. Both of these findings suggest that the visual cues provided by the black intercept trap play an important role in attraction for females. Capture rates between unbaited clear versus black intercept traps did not differ but the addition of a ultraviolet light increased trap efficacy. Intercept traps baited with light were more attractive than the commercial kairomone lure. Both olfactory and visual cues were found to play important roles in the response of S. noctilio to traps. A black trap may enhance the capture of females, whereas the addition of ultraviolet light could enhance capture for both sexes. Integrating these different components may help in developing an improved species-specific trap for S. noctilio. C1 [Sarvary, Mark A.; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Cooperband, Miriam F.] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. RP Sarvary, MA (reprint author), Cornell Univ, Dept Entomol, Garden Ave, Ithaca, NY 14853 USA. EM mas245@cornell.edu FU USDA APHIS FX We thank Sirex-wrangler Stefan Long for help with finding and cutting down Sirex-infested trees and organizing the collection of S. noctilio from emergence barrels. I. Caetano mated woodwasps with assistance from E. Morris and S. Kroll. A. Anderson and S. Andrews helped with wind tunnel trials. A. Hartness assisted in the original construction of the wind tunnel. R. Kepler and J. Liebherr assisted with reassembly and later modification of the wind tunnel. We thank Kitty Gifford and Tim Youngs from Mad Men Fridays Design for their assistance with the illustrations. We also thank the anonymous reviewers for their thoughtful comments. Funding for the present study was provided through a cooperative agreement with USDA APHIS. Mention of a commercial product does not constitute an endorsement or recommendation for its use by the USDA. NR 37 TC 4 Z9 4 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD FEB PY 2015 VL 17 IS 1 BP 29 EP 35 DI 10.1111/afe.12077 PG 7 WC Entomology SC Entomology GA AZ7XP UT WOS:000348428800004 ER PT J AU Dodds, KJ Allison, JD Miller, DR Hanavan, RP Sweeney, J AF Dodds, Kevin J. Allison, Jeremy D. Miller, Daniel R. Hanavan, Ryan P. Sweeney, Jon TI Considering species richness and rarity when selecting optimal survey traps: comparisons of semiochemical baited flight intercept traps for Cerambycidae in eastern North America SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Cerambycidae; rarity; species estimation; species richness; survey; trapping; traps ID LONGHORNED BEETLES COLEOPTERA; HOST-PLANT VOLATILES; WOOD-BORING BEETLES; TETROPIUM-FUSCUM F; MULTIPLE-FUNNEL; UNITED-STATES; PINE STANDS; BARK; PHEROMONES; BIODIVERSITY AB We compared standard multiple-funnel, modified multiple-funnel, intercept panel and canopy malaise (SLAM) traps with top and bottom collecting cups for their effectiveness (species richness, rarity, abundance) at capturing Cerambycidae in eastern North America. Experiments were conducted in New York, Louisiana, Massachusetts and Georgia in 2011 and 2012. A combination of pheromones and host volatiles chosen to match local forest types were used as lures. Species richness tended to be higher in SLAM and modified funnel traps than standard funnel and intercept panel traps. SLAM traps also captured the highest number of species, unique species, rare (species accounting for 1% of total cerambycids at a site) and singleton species at each site. Individual-based rarefaction and sample-based species accumulation curves suggested that SLAM traps are more effective for capturing cerambycid species. For many estimates, modified funnel and funnel traps were lower than SLAM traps but greater than intercept panel traps for describing cerambycid communities. Modified funnel and SLAM traps generally captured the highest abundance of cerambycids but the response of the individual subfamily and species varied by trap type. SLAM traps should be considered as a strong tool to describe cerambycid communities when used in conjunction with pheromones and host volatiles. C1 [Dodds, Kevin J.; Hanavan, Ryan P.] US Forest Serv, USDA, Northeastern Area State & Private Forestry, Durham, NH 03824 USA. [Allison, Jeremy D.] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Miller, Daniel R.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. [Sweeney, Jon] Nat Resources Canada, Canadian Forest Serv, Atlantic Forestry Ctr, Fredericton, NB E3B 5P7, Canada. RP Dodds, KJ (reprint author), US Forest Serv, USDA, Northeastern Area State & Private Forestry, 271 Mast Rd, Durham, NH 03824 USA. EM kdodds@fs.fed.us FU US Forest Service Forest Health Protection; LSU AgCenter; Natural Resources Canada - Canadian Forest Service; USDA Animal Plant Health Inspection Service FX Many individuals contributed to this work. In New York, field work was conducted by Mou Jian Lee (Cornell University) and Sarah Schoenberg (USFS Finger Lakes National Forest). Site selection and access to forest stands at the Arnot Teaching and Research Forest were provided by Peter J. Smallidge (Cornell University). Ken Gooch and Brimfield State Forest staff (Massachusetts DCR) helped us locate and access field sites at Brimfield State Forest in Massachusetts. Melanie Joy made trap collections in Massachusetts. Garret Dubois (USFS FHP) assisted with study set-up in New York and Massachusetts. In Louisiana, field work was conducted by Jessica McKenney and access to forest stands was provided by Anthony Page (Kisatchie National Forest, Catahoula Ranger District). Chris Crowe (USFS Southern Research Station) provided field assistance in Georgia. We thank Peter Mayo and Peter Silk (Canadian Forest Service, Fredericton) for synthesizing the R*R*-2,3-hexane diols and Ervin Kovacs (Contech Enterprises) for providing data on release rates of lures. Angela Maddux-Hammond (USFS FHP) sorted and identified cerambycids from New York, Massachusetts, Louisiana and Georgia. E. Richard Hoebeke (Georgia Museum of Natural History) confirmed difficult specimens. Michael J. Simmons and Tom D. Lee (University of New Hampshire) provided helpful comments on an earlier version of this manuscript. The US Forest Service Forest Health Protection, LSU AgCenter, Natural Resources Canada - Canadian Forest Service and USDA Animal Plant Health Inspection Service provided financial support. NR 70 TC 5 Z9 5 U1 2 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD FEB PY 2015 VL 17 IS 1 BP 36 EP 47 DI 10.1111/afe.12078 PG 12 WC Entomology SC Entomology GA AZ7XP UT WOS:000348428800005 ER PT J AU Hurley, BP Garnas, J Cooperband, MF AF Hurley, Brett P. Garnas, Jeff Cooperband, Miriam F. TI Assessing trap and lure effectiveness for the monitoring of Sirex noctilio SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Insect detection and monitoring; intercept panel traps; kairomone; pheromone ID WOOD-BORING INSECTS; FORESTRY PLANTATIONS; AGRILUS-PLANIPENNIS; SOUTHERN-HEMISPHERE; COLEOPTERA; PHEROMONE; MOTH; HEIGHT; CURCULIONIDAE; POPULATIONS AB Lure-baited traps are an important tool for monitoring the spread and establishment of the Sirex woodwasp Sirex noctilio. The utility of these traps, however, is limited in areas with low wasp populations as a result of the reliance on a plant volatile (kairomone) lure in the absence of an identified pheromone. Knowledge of the optimal trap type and deployment strategy is also lacking. We tested the effectiveness of a putative pheromone in baited traps, by means of a series of field trials in South Africa, over a 3-year period. We also examined the influence of lure type, trap type and trap height on capture success. The pheromone was found to be ineffective as an attractant under South African field conditions for both male and female wasps. Lure type, trap type and trap height were found to have little to no effect on female wasp catch. Given the moderately strong responses to the blend under wind tunnel and laboratory conditions, we suggest possible aspects of the biology and life history of S. noctilio that may influence lure effectiveness. The traditional black intercept panel traps with kairomone lure remains the best trap for S. noctilio, at least where populations are high. C1 [Hurley, Brett P.; Garnas, Jeff] Univ Pretoria, FABI, Dept Zool & Entomol, ZA-0083 Pretoria, South Africa. [Cooperband, Miriam F.] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. RP Hurley, BP (reprint author), Univ Pretoria, FABI, Dept Zool & Entomol, Cnr Lynnwood & Univ Rd, ZA-0083 Pretoria, South Africa. EM brett.hurley@up.ac.za RI Hurley, Brett/A-2168-2009 OI Hurley, Brett/0000-0002-8702-5547 FU USDA APHIS PPQ CPHST FX The present studywas funded by the USDA APHIS PPQ CPHST. We thank the South African forestry companies Sappi and PG Bison for permission to conduct the field trials on their land. We specifically thank Marcel Verleur (Sappi), Richard McCarthy (Sappi), Julian Chan (PG Bison) and Philip Croft (ICFR) for various logistical support. We also thank Denzil Lawrie and his team, as well as the numerous students from FABI, University of Pretoria, for assistance with setting up and monitoring the trap trials over the 3-year period. We thank Ashley Hartness for help with the preparation of materials to be tested; Tappey Jones and J. P. Lafontaine for their work on the pheromone; and John Borden and Contech for providing Flexlures to test. Mention of a commercial product does not constitute an official endorsement or approval by the USDA of any product or service to the exclusion of others that may be suitable. NR 36 TC 4 Z9 4 U1 2 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD FEB PY 2015 VL 17 IS 1 BP 64 EP 70 DI 10.1111/afe.12081 PG 7 WC Entomology SC Entomology GA AZ7XP UT WOS:000348428800008 ER PT J AU Ochiai, E Sa, QL Brogli, M Kudo, T Wang, XS Dubey, JP Suzukit, Y AF Ochiai, Eri Sa, Qila Brogli, Morgan Kudo, Tomoya Wang, Xisheng Dubey, Jitender P. Suzukit, Yasuhiro TI CXCL9 Is Important for Recruiting Immune T Cells into the Brain and Inducing an Accumulation of the T Cells to the Areas of Tachyzoite Proliferation to Prevent Reactivation of Chronic Cerebral Infection with Toxoplasma gondii SO AMERICAN JOURNAL OF PATHOLOGY LA English DT Article ID MICE GENETICALLY RESISTANT; GAMMA-MEDIATED EXPRESSION; CHEMOKINE RECEPTOR CXCR3; CENTRAL-NERVOUS-SYSTEM; IFN-GAMMA; INTERFERON-GAMMA; BALB/C MICE; CHRONIC STAGE; TNF-ALPHA; IN-VIVO AB T cells are required to maintain the latency of chronic infection with Toxoplasma gondii in the brain. Here, we examined the role of non glutamic acid-Leucine-arginine CXC chemokine CXCL9 for T-cell recruitment to prevent reactivation of infection with T. gondii. Severe combined immunodeficient (SCID) mice were infected and treated with sulfadiazine to establish a chronic infection. Immune T cells from infected wildtype mice were transferred into the SCID mice in combination with treatment with anti-CXCL9 or control sera. Three days later, sulfadiazine was discontinued to initiate reactivation of infection. Numbers of CD4(+) and CD8(+) T cells isolated from the brains were markedly less in mice treated with anti-CXCL9 serum than in mice treated with control serum at 3 days after sulfadiazine discontinuation. Amounts of tachyzoite (acute stage form of T. gondii)-specific SAG1 mRNA and numbers of foci associated with tachyzoites were significantly greater in the former than the latter at 5 days after sulfadiazine discontinuation. An accumulation of CD3(+) T cells into the areas of tachyzoite growth was significantly less frequent in the SCID mice treated with anti-CXCL9 serum than in mice treated with control serum. These results indicate that CXCL9 is crucial for recruiting immune T cells into the brain and inducing an accumulation of the T cells into the areas where tachyzoites proliferate to prevent reactivation of chronic T. gondii infection. C1 [Ochiai, Eri; Sa, Qila; Brogli, Morgan; Kudo, Tomoya; Suzukit, Yasuhiro] Univ Kentucky, Coll Med, Dept Microbiol Immunol & Mol Genet, Lexington, KY 40536 USA. [Wang, Xisheng; Suzukit, Yasuhiro] Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA. [Dubey, Jitender P.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Suzukit, Y (reprint author), Univ Kentucky, Coll Med, Dept Microbiol Immunol & Mol Genet, 800 Rose St, Lexington, KY 40536 USA. EM yasu.suzuki@uky.edu RI Ochiai, Eri/J-2226-2016 OI Ochiai, Eri/0000-0002-5629-2271 FU NIH [AI078756, AI 095032, AI077877] FX Supported in part by NIH grants AI078756, AI 095032, and AI077877. NR 58 TC 6 Z9 6 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9440 EI 1525-2191 J9 AM J PATHOL JI Am. J. Pathol. PD FEB PY 2015 VL 185 IS 2 BP 314 EP 324 DI 10.1016/j.ajpath.2014.10.003 PG 11 WC Pathology SC Pathology GA AZ8UH UT WOS:000348489400005 PM 25432064 ER PT J AU Columbus, D Fiorotto, M Davis, T AF Columbus, Daniel A. Fiorotto, Marta L. Davis, Teresa A. TI Leucine is a major regulator of muscle protein synthesis in neonates SO AMINO ACIDS LA English DT Review DE Amino acids; Leucine; Low birth weight; mTOR; Neonatal; Protein synthesis ID LOW-BIRTH-WEIGHT; HYDROXY-BETA-METHYLBUTYRATE; INITIATION-FACTOR ACTIVATION; AMINO-ACID AVAILABILITY; HUMAN SKELETAL-MUSCLE; TRANSLATION INITIATION; RESISTANCE-EXERCISE; POSTABSORPTIVE RATS; METABOLIC-RESPONSE; DIETARY-PROTEIN AB Approximately 10 % of infants born in the United States are of low birth weight. Growth failure during the neonatal period is a common occurrence in low birth weight infants due to their inability to tolerate full feeds, concerns about advancing protein supply, and high nutrient requirements for growth. An improved understanding of the nutritional regulation of growth during this critical period of postnatal growth is vital for the development of strategies to improve lean gain. Past studies with animal models have demonstrated that muscle protein synthesis is increased substantially following a meal and that this increase is due to the postprandial rise in amino acids as well as insulin. Both amino acids and insulin act independently to stimulate protein synthesis in a mammalian target of rapamycin-dependent manner. Further studies have elucidated that leucine, in particular, and its metabolites, alpha-ketoisocaproic acid and beta-hydroxy-beta-methylbutyrate, have unique anabolic properties. Supplementation with leucine, provided either parenterally or enterally, has been shown to enhance muscle protein synthesis in neonatal pigs, making it an ideal candidate for stimulating growth of low birth weight infants. C1 [Columbus, Daniel A.; Fiorotto, Marta L.; Davis, Teresa A.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Davis, T (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR-044474, AR-46308]; National Institute of Child Health and Human Development [HD-072891]; United States Department of Agriculture National Institute of Agriculture [2013-67015-20438]; USDA/ARS [6250-510000-055] FX The work was supported by National Institute of Arthritis and Musculoskeletal and Skin Diseases Grants AR-044474 (Davis) and AR-46308 (Fiorotto), National Institute of Child Health and Human Development HD-072891 (Davis), United States Department of Agriculture National Institute of Agriculture grant 2013-67015-20438 (Davis), and by the USDA/ARS under Cooperative Agreement no. 6250-510000-055 (Davis). This work is a publication of the USDA, Agricultural Research Service (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX. The contents of this publication do not necessarily reflect the views or politics of the USDA, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 90 TC 15 Z9 16 U1 5 U2 25 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0939-4451 EI 1438-2199 J9 AMINO ACIDS JI Amino Acids PD FEB PY 2015 VL 47 IS 2 BP 259 EP 270 DI 10.1007/s00726-014-1866-0 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AZ6AK UT WOS:000348300000003 PM 25408462 ER PT J AU Kavetskiy, A Yakubova, G Torbert, HA Prior, SA AF Kavetskiy, A. Yakubova, G. Torbert, H. A. Prior, S. A. TI Continuous versus pulse neutron induced gamma spectroscopy for soil carbon analysis SO APPLIED RADIATION AND ISOTOPES LA English DT Article DE Soil carbon; Soil analysis; Inelastic neutron scattering; Thermo neutron capture; Neutron generator ID RAY SPECTRA; SYSTEM AB Neutron induced gamma spectra analysis (NGA) provides a means of measuring carbon in large soil volumes without destructive sampling. Calibration of the NGA system must account for system background and the interference of other nuclei on the carbon peak at 4.43 MeV. Accounting for these factors produced measurements in agreement with theoretical considerations. The continuous NGA mode was twice as fast and just as accurate as the pulse mode, thus this mode was preferable for routine soil carbon analysis. Published by Elsevier Ltd. C1 [Kavetskiy, A.; Yakubova, G.; Torbert, H. A.; Prior, S. A.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. RP Torbert, HA (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. EM allen.torbert@ars.usda.gov NR 17 TC 2 Z9 2 U1 2 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0969-8043 J9 APPL RADIAT ISOTOPES JI Appl. Radiat. Isot. PD FEB PY 2015 VL 96 BP 139 EP 147 DI 10.1016/j.apradiso.2014.10.024 PG 9 WC Chemistry, Inorganic & Nuclear; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Chemistry; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA CA2PD UT WOS:000348749100019 PM 25497322 ER PT J AU Vincent, JB Nielsen, FH AF Vincent, John B. Nielsen, Forrest H. TI Untitled SO BIOLOGICAL TRACE ELEMENT RESEARCH LA English DT Editorial Material C1 [Vincent, John B.] Univ Alabama, Dept Chem, Tuscaloosa, AL 35487 USA. [Nielsen, Forrest H.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Vincent, JB (reprint author), Univ Alabama, Dept Chem, Box 870336, Tuscaloosa, AL 35487 USA. EM jvincent@ua.edu; frostynielsen@yahoo.com NR 0 TC 0 Z9 0 U1 0 U2 1 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0163-4984 EI 1559-0720 J9 BIOL TRACE ELEM RES JI Biol. Trace Elem. Res. PD FEB PY 2015 VL 163 IS 1-2 BP 1 EP 1 DI 10.1007/s12011-014-0214-2 PG 1 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA AZ3EC UT WOS:000348109500001 PM 25524524 ER PT J AU Keremane, ML Ramadugu, C Rodriguez, E Kubota, R Shibata, S Hall, DG Roose, ML Jenkins, D Lee, RF AF Keremane, Manjunath L. Ramadugu, Chandrika Rodriguez, Esteban Kubota, Ryo Shibata, Scott Hall, David G. Roose, Mikeal L. Jenkins, Daniel Lee, Richard F. TI A rapid field detection system for citrus huanglongbing associated 'Candidatus Liberibacter asiaticus' from the psyllid vector, Diaphorina citri Kuwayama and its implications in disease management SO CROP PROTECTION LA English DT Article DE Huanglongbing; Field detection; LAMP; Citrus greening; Asian citrus psyllid ID MEDIATED ISOTHERMAL AMPLIFICATION; REAL-TIME PCR; GREENING DISEASE; HEMIPTERA PSYLLIDAE; SENSITIVE DETECTION; SOLANACEARUM; FLORIDA; TRANSMISSION; PLANTS; LAMP AB 'Candidatus Liberibacter asiaticus' (Las), associated with citrus huanglongbing (HLB or citrus greening) is spread by Diaphorina citri Kuwayama, the Asian citrus psyllid. Psyllids can be tested to assess the prevalence of Las in a population and for early detection of HLB in new areas being invaded by the psyllid. In some cases, large numbers of psyllids may need to be tested, thus there is a need for rapid and inexpensive field detection methodology. We report here on the development of a field detection kit for testing psyllids for Las using loop-mediated amplification technology (LAMP). Six samples with pools of 1-10 psyllids plus a positive and negative control can be tested at a time in about 30 min; 10 min for crude extraction and 20 min for target DNA amplification. The LAMP assays are conducted in a Smart-DART (TM) detection unit which is operated from an Android device. The LAMP detection method for Las is about 100 times more sensitive than the traditional real time PCR method. In addition to field testing of psyllids for Las, the methodology was validated as effective for identifying Las in plant DNA extractions. In California, where the psyllid has only recently invaded, participation of a large number of growers and extension workers in field detection may facilitate rapid containment efforts should Las be found. In areas where the disease epidemic is already in the initial stages, large scale testing can be helpful for effective disease management. The LAMP technology lends itself well in such situations. Published by Elsevier Ltd. C1 [Keremane, Manjunath L.; Rodriguez, Esteban; Lee, Richard F.] USDA ARS, Riverside, CA 92507 USA. [Ramadugu, Chandrika; Roose, Mikeal L.] Univ Calif Riverside, Riverside, CA 92521 USA. [Kubota, Ryo; Shibata, Scott] Diagenetix, Honolulu, HI USA. [Hall, David G.] US Hort Lab, Ft Pierce, FL USA. [Jenkins, Daniel] Univ Hawaii, Honolulu, HI 96822 USA. RP Keremane, ML (reprint author), USDA ARS, 1060 Martin Luther King Blvd, Riverside, CA 92507 USA. EM Manjunath.keremane@ars.usda.gov FU California Citrus Nursery Board [CNAB 2013] FX Funding from California Citrus Nursery Board, CNAB 2013 Lee is gratefully acknowledged. The project was developed after discussion with some citrus growers and nurserymen in California. The funding agency had no involvement in the study design, analyses or interpretation of data. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 43 TC 9 Z9 9 U1 4 U2 57 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD FEB PY 2015 VL 68 BP 41 EP 48 DI 10.1016/j.cropro.2014.10.026 PG 8 WC Agronomy SC Agriculture GA AZ5IS UT WOS:000348255600005 ER PT J AU Li, ZW Zhang, GH Yu, XX Liu, QJ Zhang, XC AF Li, Zhernwei Zhang, Guanghui Yu, Xingxiu Liu, Qianjin Zhang, X. C. TI Phosphorus loss and its estimation in a small watershed of the Yimeng mountainous area, China SO ENVIRONMENTAL EARTH SCIENCES LA English DT Article DE Rainfall; Runoff; Sediment; Phosphorus loss; Agricultural watershed ID ADJACENT AGRICULTURAL WATERSHEDS; CYPRESS CHAMAECYPARIS-OBTUSA; SURFACE RUNOFF; CHEMICAL-TRANSFER; NUTRIENT LOSSES; RAINFALL INTENSITY; STORMWATER RUNOFF; SOIL-EROSION; STREAM-FLOW; SEDIMENT AB Non-point source pollution is severe in the Yimeng Mountainous Area of China. Few studies have been conducted to identify and predict phosphorus loss at a watershed scale in this region. The objectives of this study were to identify the characteristics of phosphorus loss and further to develop regression models to estimate phosphorus losses of different forms based on the dataset of measured rainfall, runoff, and sediment of 24 rainfall events during the period of 2010-2011 in a typical small watershed from Yimeng Mountainous Area. The results revealed that phosphorus (P) loss differed considerably with phosphorus forms. The dissolved inorganic phosphorus (DIP) was the dominant form of the total dissolved phosphorus (TDP) loss by runoff, accounting for 72 %. The ratio of particulate phosphorus (PP) to total phosphorus (TP) was 65 %, which indicated that most of phosphorus loss was transported by eroded sediment. Similar to soil loss, rainfall properties influenced phosphorus loss greatly and most of the phosphorus loss was produced by only a few heavy storms. The concentrations of different phosphorus forms during a rainfall event varied with similar trends and reached the maxima prior to the peak runoff. Phosphorus losses of different forms were influenced by rainfall kinetic energy, runoff volume, and sediment loss significantly. DIP, TDP, and TP could be predicted with rainfall kinetic energy and runoff volume satisfactorily (NSE >= 0.92). PP was estimated with rainfall kinetic energy, runoff volume, and sediment loss of rainfall event well (NSE = 0.90). Further studies are necessary to assess the performance of the developed models under different conditions. C1 [Li, Zhernwei; Zhang, Guanghui] Chinese Acad Sci, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. [Li, Zhernwei; Zhang, Guanghui] Minist Water Resources, Yangling 712100, Shaanxi, Peoples R China. [Li, Zhernwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Zhang, Guanghui] Beijing Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Yu, Xingxiu; Liu, Qianjin] Linyi Univ, Shandong Prov Key Lab Soil Conservat & Environm P, Linyi 276005, Peoples R China. [Zhang, X. C.] ARS, USDA, Grazinglands Res Lab, El Reno, OK USA. RP Zhang, GH (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. EM ghzhang@bnu.edu.cn FU Hundred Talents Project of the Chinese Academy of Sciences; National natural Foundation [41271287] FX Financial assistance for this work was provided by the Hundred Talents Project of the Chinese Academy of Sciences and the National natural Foundation (41271287). NR 49 TC 2 Z9 2 U1 1 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1866-6280 EI 1866-6299 J9 ENVIRON EARTH SCI JI Environ. Earth Sci. PD FEB PY 2015 VL 73 IS 3 BP 1205 EP 1216 DI 10.1007/s12665-014-3475-3 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA AZ6PI UT WOS:000348340900024 ER PT J AU Munns, KD Selinger, LB Stanford, K Guan, LL Callaway, TR McAllister, TA AF Munns, Krysty D. Selinger, L. Brent Stanford, Kim Guan, Leluo Callaway, Todd R. McAllister, Tim A. TI Perspectives on Super-Shedding of Escherichia coli O157:H7 by Cattle SO Foodborne Pathogens and Disease LA English DT Review ID COMMERCIAL FEEDLOT CATTLE; SHIGA TOXIN; RECTOANAL-JUNCTION; BIOFILM FORMATION; BEEF-CATTLE; PREHARVEST INTERVENTIONS; SALMONELLA-ENTERICA; O157-H7 POPULATIONS; ANIMAL VARIATION; GENOME SEQUENCE AB Escherichia coli O157:H7 is a foodborne pathogen that causes illness in humans worldwide. Cattle are the primary reservoir of this bacterium, with the concentration and frequency of E. coli O157:H7 shedding varying greatly among individuals. The term "super-shedder" has been applied to cattle that shed concentrations of E. coli O157:H7 >= 10(4) colony-forming units/g feces. Super-shedders have been reported to have a substantial impact on the prevalence and transmission of E. coli O157:H7 in the environment. The specific factors responsible for super-shedding are unknown, but are presumably mediated by characteristics of the bacterium, animal host, and environment. Super-shedding is sporadic and inconsistent, suggesting that biofilms of E. coli O157:H7 colonizing the intestinal epithelium in cattle are intermittently released into feces. Phenotypic and genotypic differences have been noted in E. coli O157:H7 recovered from super-shedders as compared to low-shedding cattle, including differences in phage type (PT21/28), carbon utilization, degree of clonal relatedness, tir polymorphisms, and differences in the presence of stx2a and stx2c, as well as antiterminator Q gene alleles. There is also some evidence to support that the native fecal microbiome is distinct between super-shedders and low-shedders and that low-shedders have higher levels of lytic phage within feces. Consequently, conditions within the host may determine whether E. coli O157:H7 can proliferate sufficiently for the host to obtain super-shedding status. Targeting super-shedders for mitigation of E. coli O157:H7 has been proposed as a means of reducing the incidence and spread of this pathogen to the environment. If super-shedders could be easily identified, strategies such as bacteriophage therapy, probiotics, vaccination, or dietary inclusion of plant secondary compounds could be specifically targeted at this subpopulation. Evidence that super-shedder isolates share a commonality with isolates linked to human illness makes it imperative that the etiology of this phenomenon be characterized. C1 [Munns, Krysty D.; Selinger, L. Brent; McAllister, Tim A.] Agr & Agri Food Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada. [Munns, Krysty D.] Univ Lethbridge, Dept Biol Sci, Lethbridge, AB T1K 3M4, Canada. [Stanford, Kim] Alberta Agr & Rural Dev, Lethbridge, AB, Canada. [Guan, Leluo] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. [Callaway, Todd R.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX USA. RP McAllister, TA (reprint author), Agr & Agri Food Canada, Lethbridge Res Ctr, 5403 1st Ave South, Lethbridge, AB T1J 4B1, Canada. EM tim.mcallister@agr.gc.ca FU Genome Alberta; Alberta Livestock Meat Agency FX The authors wish to express their appreciation to Genome Alberta and the Alberta Livestock Meat Agency for financial support for some of the research that was conducted in their laboratories and reported within this review article. NR 123 TC 6 Z9 7 U1 2 U2 30 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD FEB 1 PY 2015 VL 12 IS 2 BP 89 EP 103 DI 10.1089/fpd.2014.1829 PG 15 WC Food Science & Technology SC Food Science & Technology GA CA6GI UT WOS:000349008100001 PM 25514549 ER PT J AU Clotilde, LM Salvador, A Bernard, C Lin, A Lauzon, C Muldoon, M Xu, YC Carter, JM AF Clotilde, Laurie M. Salvador, Alexandra Bernard, Clay Lin, Andrew Lauzon, Carol Muldoon, Mark Xu, Yichun Carter, John Mark TI Comparison of Multiplex Immunochemical and Molecular Serotyping Methods for Shiga Toxin-Producing Escherichia coli SO Foodborne Pathogens and Disease LA English DT Article ID BEEF CARCASSES; UNITED-STATES; PREVALENCE; CATTLE AB Traditionally, serotyping of Escherichia coli has been performed via slide agglutination methods using antisera. More recently, multiplex immunoassays and "molecular serotyping" via polymerase chain reaction (PCR) have been validated for this purpose. In this study, the serogroups of 161 Shiga toxin-producing Escherichia coli (STEC) strains isolated from fecal samples of California cattle were typed by conventional methods using antisera as well as two newly developed multiplex PCR- and antibody-based microbead assays using the Luminex technology. Using the Luminex assays, we were capable of serotyping 11 STEC isolates that were previously determined untypeable for the O antigen by conventional methods using antisera. Except for 14 isolates, results from the 2 Luminex assays agreed. C1 [Clotilde, Laurie M.] MagArray, Milpitas, CA USA. [Salvador, Alexandra; Lauzon, Carol] Calif State Univ Hayward, Dept Biol Sci, Hayward, CA 94542 USA. [Salvador, Alexandra; Bernard, Clay; Carter, John Mark] ARS, USDA, Albany, CA USA. [Lin, Andrew] US FDA, Alameda, CA USA. [Muldoon, Mark] Romer Labs Technol Inc, Newark, DE USA. [Xu, Yichun] SDIX, Newark, DE USA. RP Carter, JM (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM j.mark.carter@usda.ars.gov RI Carter, John Mark/K-2485-2015 OI Carter, John Mark/0000-0001-8251-4168 FU FDA Commissioner's Fellow Program; San Francisco District Laboratory; USDA FX The authors would like to thank FDA Commissioner's Fellow Program and San Francisco District Laboratory for supporting this research. USDA funding was administrated through the Agricultural Research Service, National Program 108 Food Safety. NR 20 TC 2 Z9 2 U1 1 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD FEB 1 PY 2015 VL 12 IS 2 BP 118 EP 121 DI 10.1089/fpd.2014.1827 PG 4 WC Food Science & Technology SC Food Science & Technology GA CA6GI UT WOS:000349008100004 PM 25646966 ER PT J AU Teixeira, JEC Weldekidan, T de Leon, N Flint-Garcia, S Holland, JB Lauter, N Murray, SC Xu, W Hessel, DA Kleintop, AE Hawk, JA Hallauer, A Wisser, RJ AF Teixeira, J. E. C. Weldekidan, T. de Leon, N. Flint-Garcia, S. Holland, J. B. Lauter, N. Murray, S. C. Xu, W. Hessel, D. A. Kleintop, A. E. Hawk, J. A. Hallauer, A. Wisser, R. J. TI Hallauer's Tuson: a decade of selection for tropical-to-temperate phenological adaptation in maize SO HEREDITY LA English DT Article ID FLOWERING TIME; PHOTOPERIOD SENSITIVITY; ENVIRONMENT INTERACTION; ARTIFICIAL SELECTION; GENETIC ARCHITECTURE; DNA MICROSATELLITES; TASSEL INITIATION; CLIMATE-CHANGE; INBRED LINES; FIELD TRIALS AB Crop species exhibit an astounding capacity for environmental adaptation, but genetic bottlenecks resulting from intense selection for adaptation and productivity can lead to a genetically vulnerable crop. Improving the genetic resiliency of temperate maize depends upon the use of tropical germplasm, which harbors a rich source of natural allelic diversity. Here, the adaptation process was studied in a tropical maize population subjected to 10 recurrent generations of directional selection for early flowering in a single temperate environment in Iowa, USA. We evaluated the response to this selection across a geographical range spanning from 43.05 degrees (WI) to 18.00 degrees (PR) latitude. The capacity for an all-tropical maize population to become adapted to a temperate environment was revealed in a marked fashion: on average, families from generation 10 flowered 20 days earlier than families in generation 0, with a nine-day separation between the latest generation 10 family and the earliest generation 0 family. Results suggest that adaptation was primarily due to selection on genetic main effects tailored to temperature-dependent plasticity in flowering time. Genotype-by-environment interactions represented a relatively small component of the phenotypic variation in flowering time, but were sufficient to produce a signature of localized adaptation that radiated latitudinally, in partial association with daylength and temperature, from the original location of selection. Furthermore, the original population exhibited a maladaptive syndrome including excessive ear and plant heights along with later flowering; this was reduced in frequency by selection for flowering time. C1 [Teixeira, J. E. C.; Weldekidan, T.; Kleintop, A. E.; Hawk, J. A.; Wisser, R. J.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [de Leon, N.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Flint-Garcia, S.] USDA ARS, Columbia, MO USA. [Flint-Garcia, S.] Univ Missouri, Div Plant Sci, Columbia, MO USA. [Holland, J. B.] USDA ARS, Raleigh, NC 27695 USA. [Holland, J. B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Lauter, N.] USDA ARS, Ames, IA USA. [Lauter, N.; Hessel, D. A.] Iowa State Univ, Interdept Genet Grad Program, Ames, IA USA. [Murray, S. C.; Xu, W.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Xu, W.] Texas A&M AgriLife Res, Lubbock Res & Extens Ctr, Lubbock, TX USA. [Hallauer, A.] Iowa State Univ, Dept Agron, Ames, IA USA. RP Wisser, RJ (reprint author), Univ Delaware, Dept Plant & Soil Sci, 531 South Coll Ave,152 Townsend Hall, Newark, DE 19716 USA. EM rjw@udel.edu FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture (Agriculture and Natural Resources Science for Climate Variability and Change Program) [2011-67003-30342]; Hatch Act funds; US Department of Agriculture-Agricultural Research Service FX This project was supported by Agriculture and Food Research Initiative Competitive Grant No. 2011-67003-30342 from the USDA National Institute of Food and Agriculture (Agriculture and Natural Resources Science for Climate Variability and Change Program), Hatch Act funds and the US Department of Agriculture-Agricultural Research Service. The investigators also wish to thank students and technical support staff that assisted with collection of this data. NR 62 TC 2 Z9 2 U1 2 U2 17 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD FEB PY 2015 VL 114 IS 2 BP 229 EP 240 DI 10.1038/hdy.2014.90 PG 12 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA AZ2OB UT WOS:000348071600011 PM 25370213 ER PT J AU Macciotta, NPP Dimauro, C Null, DJ Gaspa, G Cellesi, M Cole, JB AF Macciotta, N. P. P. Dimauro, C. Null, D. J. Gaspa, G. Cellesi, M. Cole, J. B. TI Dissection of genomic correlation matrices of US Holsteins using multivariate factor analysis SO JOURNAL OF ANIMAL BREEDING AND GENETICS LA English DT Article DE Animal breeding; chromosome; genomic selection ID COMMON PRINCIPAL COMPONENTS; SINGLE NUCLEOTIDE POLYMORPHISMS; QUANTITATIVE TRAIT LOCUS; MILK-PRODUCTION; DAIRY-CATTLE; WIDE ASSOCIATION; CONFORMATION TRAITS; VISUALIZATION; REPRODUCTION; SELECTION AB The aim of this study was to compare correlation matrices between direct genomic predictions for 31 traits at the genomic and chromosomal levels in US Holstein bulls. Multivariate factor analysis carried out at the genome level identified seven factors associated with conformation, longevity, yield, feet and legs, fat and protein content traits. Some differences were found at the chromosome level; variations in covariance structure on BTA 6, 14, 18 and 20 were interpreted as evidence of segregating QTL for different groups of traits. For example, milk yield and composition tended to join in a single factor on BTA 14, which is known to harbour the DGAT1 locus that affects these traits. Another example was on BTA 18, where a factor strongly correlated with sire calving ease and conformation traits was identified. It is known that in US Holstein, there is a segregating QTL on BTA18 influencing these traits. Moreover, a possible candidate gene for daughter pregnancy rate was suggested for BTA28. The methodology proposed in this study could be used to identify individual chromosomes, which have covariance structures that differ from the overall (whole genome) covariance structure. Such differences can be difficult to detect when a large number of traits are evaluated, and covariances may be affected by QTL that do not have large allele substitution effects. C1 [Macciotta, N. P. P.; Dimauro, C.; Gaspa, G.; Cellesi, M.] Univ Sassari, Dipartimento Agr, Sez Sci Zootecn, I-07100 Sassari, Italy. [Null, D. J.; Cole, J. B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD USA. RP Macciotta, NPP (reprint author), Univ Sassari, Dipartimento Agr, Sez Sci Zootecn, Via Nicola 9, I-07100 Sassari, Italy. EM macciott@uniss.it RI Potapyuk, Iryna/Q-7411-2016; Peredera, Roman/Q-7457-2016; Якушева, Татьяна/G-5068-2015; gaspa, giustino/J-5696-2015; Macciotta, Nicolo Pietro Paolo/J-8226-2015; Kogut, Vladymir/N-7367-2015; Vakaliuk, Tetiana/C-3650-2016; Butovskyi, Iegor/J-6505-2015; Cole, John/J-8571-2014 OI Potapyuk, Iryna/0000-0002-1051-0114; Peredera, Roman/0000-0002-9906-1211; Якушева, Татьяна/0000-0002-7635-7327; gaspa, giustino/0000-0003-1604-8917; Macciotta, Nicolo Pietro Paolo/0000-0001-5504-9459; Kogut, Vladymir/0000-0003-4431-256X; Vakaliuk, Tetiana/0000-0001-6825-4697; Butovskyi, Iegor/0000-0002-5260-4952; Cole, John/0000-0003-1242-4401 FU project 'Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information' [1265-31000-096-00]; INNOVAGEN project of the Italian Ministry of Agriculture FX The cooperation of the Council on Dairy Cattle Breeding (Reynoldsburg, OH) in supplying pedigree, performance and genotypic data for US dairy cattle is acknowledged. J.B. Cole and D.J. Null were supported by appropriated project 1265-31000-096-00, 'Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information'. The research was also funded by the INNOVAGEN project of the Italian Ministry of Agriculture. NR 36 TC 3 Z9 3 U1 0 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2668 EI 1439-0388 J9 J ANIM BREED GENET JI J. Anim. Breed. Genet. PD FEB PY 2015 VL 132 IS 1 BP 9 EP 20 DI 10.1111/jbg.12113 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA CA1KF UT WOS:000348670800003 PM 25100067 ER PT J AU Milillo, SR Lungu, B O'Bryan, CA Dowd, SE Muthaiyan, A Johnson, MG Ricke, SC AF Milillo, S. R. Lungu, B. O'Bryan, C. A. Dowd, S. E. Muthaiyan, A. Johnson, M. G. Ricke, S. C. TI Listeria monocytogenes batch culture growth response to metabolic inhibitors SO JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES LA English DT Article DE sodium arsenate.; Listeria monocytogenes; metabolic inhibitors; potassium cyanide; iodoacetate ID STRESS RESISTANCE; ACID TOLERANCE; SIGMA(B); VIRULENCE; SURVIVAL; ENERGY; NISIN; MODEL AB In certain environments nutrient and energy sources available to microorganisms can be limited. Foodborne pathogens must efficiently adapt in order to be successfully transmitted through the food chain to their hosts. For the intracellular foodborne pathogen Listeria monocytogenes, little is known regarding its response to nutrient/energy-limiting conditions. The alternative stress responsive sigma factor sigma(B) has been reported to contribute to survival under specific stresses. Therefore, the effects of several metabolic inhibitors on growth of L. monocytogenes wild-type and a Delta sigB mutant were examined. In the absence of inhibitors, both strains reached stationary phase after 18h at 23 degrees C and 10h at 37 degrees C. All of the metabolic inhibitors slowed growth of either strain, with few differences observed among the different inhibitors. C1 [Milillo, S. R.; Lungu, B.; O'Bryan, C. A.; Muthaiyan, A.; Johnson, M. G.; Ricke, S. C.] Univ Arkansas, Dept Food Sci, Fayetteville, AR 72704 USA. [Milillo, S. R.; Lungu, B.; O'Bryan, C. A.; Muthaiyan, A.; Johnson, M. G.; Ricke, S. C.] Univ Arkansas, Ctr Food Safety, Fayetteville, AR 72704 USA. [Milillo, S. R.] Penn State Univ, Dept Food Sci, University Pk, PA 16802 USA. [Dowd, S. E.] ARS, USDA, LIRU, Lubbock, TX USA. RP Ricke, SC (reprint author), Univ Arkansas, Dept Food Sci, 2650 N Young Ave, Fayetteville, AR 72704 USA. EM sricke@uark.edu FU USDA-Food Safety Consortium; USDA/CSREES NRI funding program [2008-35201-04637]; Department of Food Science at the University of Arkansas FX This study was supported in part by grants from the USDA-Food Safety Consortium and the USDA/CSREES NRI funding program (award no. 2008-35201-04637) as well as the Department of Food Science at the University of Arkansas. NR 21 TC 0 Z9 0 U1 0 U2 3 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0360-1234 EI 1532-4109 J9 J ENVIRON SCI HEAL B JI J. Environ. Sci. Health Part B-Pestic. Contam. Agric. Wastes PD FEB 1 PY 2015 VL 50 IS 2 BP 146 EP 150 DI 10.1080/03601234.2015.975626 PG 5 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA CA0AY UT WOS:000348578000009 PM 25587784 ER PT J AU O'Neil, CE Nicklas, TA Liu, Y Berenson, GS AF O'Neil, C. E. Nicklas, T. A. Liu, Y. Berenson, G. S. TI Candy consumption in childhood is not predictive of weight, adiposity measures or cardiovascular risk factors in young adults: the Bogalusa Heart Study SO JOURNAL OF HUMAN NUTRITION AND DIETETICS LA English DT Article DE adults; Bogalusa Heart Study; candy consumption; cardiovascular risk factors; children; weight/adiposity ID BODY-MASS INDEX; NUTRITION EXAMINATION SURVEY; FOOD FREQUENCY QUESTIONNAIRE; QUALITY-OF-LIFE; METABOLIC SYNDROME; BLOOD-PRESSURE; NATIONAL-HEALTH; UNITED-STATES; ESSENTIAL-HYPERTENSION; SWEETENED BEVERAGES AB Background: There are limited data available on the longitudinal relationship between candy consumption by children on weight and other cardiovascular risk factors (CVRF) in young adults. The present study investigated whether candy consumption in children was predictive of weight and CVRF in young adults. Methods: A longitudinal sample of children 10 years (n = 355; 61% females; 71% European-Americans, 29% African-Americans) who participated in cross-sectional surveys from 1973 to 1984 (baseline) and in one of two surveys (follow-ups) as young adults [19-38 years; mean (SD) = 23.6 (2.6) years] in Bogalusa, LA, were studied. Dietary data were collected using 24-h dietary recalls at baseline and at one follow-up survey; a food frequency questionnaire was used in the other follow-up survey. Candy consumers were those consuming any amount of candy. Candy consumption was calculated (g day(-1)) from baseline 24-h dietary recalls, and was used as a covariate in the adjusted linear mixed models. Dependent variables included body mass index (BMI) and CVRF measured in young adults. Results: At baseline, 92% of children reported consuming candy [46 (45) g day(-1)]; the percentage decreased to 67% [20 (30) g day(-1)] at follow-up. No longitudinal relationship was shown between baseline candy consumption and BMI or CVRF in young adults, suggesting that candy consumption was not predictive of health risks later in life. Conclusions: The consumption of nutrient rich foods consistent with dietary recommendations is important, although modest amounts of candy can be added to the diet without potential adverse long-term consequences to weight or CVRF. Additional studies are needed to confirm these results. C1 [O'Neil, C. E.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Nicklas, T. A.; Liu, Y.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Berenson, G. S.] Tulane Univ, Sch Publ Hlth & Trop Med, Dept Epidemiol, New Orleans, LA USA. RP O'Neil, CE (reprint author), Louisiana State Univ, Ctr Agr, 261 Knapp Hall,110 LSU Union Sq, Baton Rouge, LA 70803 USA. EM coneil1@lsu.edu FU National Institute of Child Health and Human Development [HD-061437, HD-062783]; American Heart Association [0855082E]; National Institute on Aging [AG-16592]; National Confectioners Association; USDA/ARS [58-62506-003]; USDA Hatch Project LAB [93951] FX The Bogalusa Heart Study was supported by grants HD-061437 and HD-062783 from the National Institute of Child Health and Human Development; 0855082E from American Heart Association; and AG-16592 from the National Institute on Aging. Additional support for this study was obtained from the National Confectioners Association and the USDA/ARS through specific cooperative agreement 58-62506-003. Partial support was also received from the USDA Hatch Project LAB 93951. The funding sources had no input into the study design or the interpretation of the results. NR 83 TC 2 Z9 2 U1 1 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0952-3871 EI 1365-277X J9 J HUM NUTR DIET JI J. Hum. Nutr. Diet. PD FEB PY 2015 VL 28 SU 2 SI SI BP 59 EP 69 DI 10.1111/jhn.12200 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AZ6DN UT WOS:000348308900007 PM 24382141 ER PT J AU Liu, CK Latona, NP Taylor, MM Aldema-Ramos, ML AF Liu, Cheng-Kung Latona, Nicholas P. Taylor, Maryann M. Aldema-Ramos, Mila L. TI BIOBASED FILMS PREPARED FROM COLLAGEN SOLUTIONS DERIVED FROM UN-TANNED HIDES SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID IMPROVED MECHANICAL-PROPERTIES; FIBROUS NETWORKS; COMPOSITE FILMS; CROSS-LINKING; POLY(LACTIC ACID); IONIC LIQUID; PECTIN; FIBERS AB The U.S. hide and leather industries are facing challenges of meeting environmental imperatives; quantifying, maintaining, and improving current hides and leather product quality; developing new processes and products; and improving utilization of waste. One of our contributions to address these ongoing challenges is to develop innovative uses and novel biobased products from hides to improve prospective markets and to secure a viable future for hides and leather industries. We had previously investigated the production of nonwoven and green composites from collagen fiber networks, which were extracted from un-tanned hides and from tannery solid wastes, such as splits or trimmings. Recently, we focused on preparing biobased films from un-tanned; specifically limed hides, which have potential commercial applications in medical care and food packaging. Collagen fiber networks were obtained from hides that have been processed to remove the noncollagenous materials through the hair removal and liming steps. We also focused on understanding the effects of processing steps such as bating and crosslinking treatments on the morphology and physical properties of biobased films from un-tanned hides. Results showed that the concentration of collagen solution and the methods of crosslinking with glutaraldehyde during the film formation process have significant effects on the properties of resultant films. Higher concentrations of collagen and addition of glutaraldehyde crosslinkers after solidification of the films yielded better mechanical properties. The encouraging results of this ongoing research are instrumental to produce biobased films, which have wide applications in both the medical field due to good biocompatibility and the food packaging because of excellent mechanical properties and acceptable edibility. C1 [Liu, Cheng-Kung; Latona, Nicholas P.; Taylor, Maryann M.; Aldema-Ramos, Mila L.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Liu, CK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E MERMAID LANE, Wyndmoor, PA 19038 USA. EM ChengKung.Liu@ars.usda.gov NR 21 TC 1 Z9 1 U1 2 U2 12 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD FEB PY 2015 VL 110 IS 2 BP 25 EP 32 PG 8 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA CA5UJ UT WOS:000348973000001 ER PT J AU Keizerweerd, AT Chandra, A Grisham, MP AF Keizerweerd, Amber T. Chandra, Amaresh Grisham, Michael P. TI Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Sugarcane mosaic virus and Sorghum mosaic virus in sugarcane SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE RT-LAMP; Sugarcane mosaic virus; Sorghum mosaic virus; RT-PCR; Sensitivity ID TOBACCO ETCH VIRUS; RAPID DETECTION; STRAINS; DISEASE; MAIZE; RNA; PCR AB A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) in sugarcane. Six sets of four primers corresponding to the conserved coat protein gene were designed and tested for each virus. Three primer sets designed for detecting SCMV and four for detecting SrMV were successful in the RT-LAMP assay. The effective primer sets were not only specific for their target virus, but also able to detect multiple virus strains. The magnesium sulfate concentration of the reaction solution was optimized, with both viruses requiring a minimum of 5 mM for detection. The sensitivity of this RT-LAMP assay was less than that of conventional and real-time RT-PCR. Published by Elsevier B.V. C1 [Keizerweerd, Amber T.; Chandra, Amaresh; Grisham, Michael P.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. [Chandra, Amaresh] Indian Inst Sugarcane Res, Lucknow 226002, Uttar Pradesh, India. RP Keizerweerd, AT (reprint author), USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. EM amber.keizerweerd@ars.usda.gov FU USDA; ARS; Office of National Programs; American Sugar Cane League of the U.S.A, Inc. FX Mention of a trademark, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable. This study was supported in part by grants from the USDA, ARS, Office of National Programs and the American Sugar Cane League of the U.S.A, Inc. Dr. Duncan Clark of OptiGene Limited is appreciated for his technical assistance in primer design. NR 27 TC 9 Z9 10 U1 3 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD FEB PY 2015 VL 212 BP 23 EP 29 DI 10.1016/j.jviromet.2014.10.013 PG 7 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA CA0PY UT WOS:000348620400005 PM 25445794 ER PT J AU Snyder, AK Hinshaw, JM Welch, TJ AF Snyder, A. K. Hinshaw, J. M. Welch, T. J. TI Diagnostic tools for rapid detection and quantification of Weissella ceti NC36 infections in rainbow trout SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE aquaculture; diagnostic tool; rainbow trout; Weissella ceti strain NC36; Weissellosis AB Weissellosis of rainbow trout is caused by the Gram-positive bacteria Weissella ceti and has been reported in China, Brazil and the United States. This disease can result in high mortality in market-sized fish and thus can cause significant economic loss. Thus far, phenotypic characterization and 16S rRNA sequencing have been used to confirm a Weissellosis diagnosis. Here, we present the development of PCR-based diagnostic tools for the rapid identification and quantification of W.ceti within bacteriological culture and infected tissues. A duplex PCR, which amplifies both genus- and strain-specific targets, positively identifies isolates as W.ceti NC36. A qPCR assay was also developed to quantify pathogen load from infected tissues, using a W.ceti NC36 unique locus. A proof of concept study was performed to demonstrate that quantification using traditional plate count methods and qPCR were significantly correlated when assessed from infected brain and spleen tissue. These tools were also used to confirm diagnosis of Weissellosis in a commercial rainbow trout farm during an outbreak investigation. These are the first diagnostic tools developed for identification and quantification of W.ceti infection within rainbow trout, contributing to rapid Weissellosis diagnosis, enhanced pathogen surveillance and epidemiological studies. Significance and Impact of the StudyWeissellosis is a rapidly emerging infectious disease of farmed rainbow trout caused by Weissella ceti, a Gram-positive lactic-acid bacterium. This disease can cause significant economic loss, and thus, efforts to limit its spread are urgently needed and are dependent on the development of diagnostic tools. The article presents the development of PCR-based diagnostic assays for the rapid identification of W.ceti and for quantification of pathogen load directly from tissues without the need for bacterial amplification or isolation. These tools are critical for rapid confirmation of Weissellosis diagnosis and for studying the epidemiology of this emerging pathogen. C1 [Snyder, A. K.; Welch, T. J.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. [Hinshaw, J. M.] N Carolina State Univ, Dept Appl Ecol, Mills River, NC USA. RP Welch, TJ (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM tim.welch@ars.usda.gov FU USDA/ARS CRIS [1930-32000-005-00] FX We acknowledge the technical contributions from Jennifer Lipscomb, Travis Moreland, Skip Thompson and Geoffrey Nichols. We thank Ben LaFrentz for critical comments of the manuscript. This work is supported by the USDA/ARS CRIS Project 1930-32000-005-00. Trade names or commercial products given in this publication are solely for the purpose of providing specific information and do not imply recommendation or endorsement by the U.S Department of Agriculture. USDA is an equal opportunity employer. NR 12 TC 3 Z9 3 U1 2 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD FEB PY 2015 VL 60 IS 2 BP 103 EP 110 DI 10.1111/lam.12365 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA2HA UT WOS:000348728200001 PM 25470116 ER PT J AU Jackson, CR Kariyawasam, S Borst, LB Frye, JG Barrett, JB Hiott, LM Woodley, TA AF Jackson, C. R. Kariyawasam, S. Borst, L. B. Frye, J. G. Barrett, J. B. Hiott, L. M. Woodley, T. A. TI Antimicrobial resistance, virulence determinants and genetic profiles of clinical and nonclinical Enterococcus cecorum from poultry SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE antimicrobial resistance; Enterococcus cecorum; genetic relatedness; poultry; virulence ID MOLECULAR EPIDEMIOLOGY; BROILER BREEDERS; MULTIPLEX PCR; CHICKENS; FAECALIS; IDENTIFICATION; OSTEOMYELITIS; SPONDYLITIS; FAECIUM; CANADA AB Enterococcus cecorum has been implicated as a possible cause of disease in poultry. However, the characteristics that contribute to pathogenesis of Ent.cecorum in poultry have not been defined. In this study, Ent.cecorum from carcass rinsates (n=75) and diseased broilers and broiler breeders (n=30) were compared based upon antimicrobial resistance phenotype, the presence of virulence determinants and genetic relatedness using pulsed-field gel electrophoresis (PFGE). Of the 16 antimicrobials tested, Ent.cecorum from carcass rinsates and clinical cases were resistant to ten and six of the antimicrobials, respectively. The majority of Ent.cecorum from carcass rinsates was resistant to lincomycin (54/75; 72%) and tetracycline (46/75; 613%) while the highest level of resistance among clinical Ent.cecorum was to tetracycline (22/30; 733%) and erythromycin (11/30; 367%). Multidrug resistance (resistance to 2 antimicrobials) was identified in Ent.cecorum from carcass rinsates (53/75; 707%) and diseased poultry (18/30; 60%). Of the virulence determinants tested, efaAfm was present in almost all of the isolates (104/105; 99%). Using PFGE, the majority of clinical isolates clustered together; however, a few clinical isolates grouped with Ent.cecorum from carcass rinsates. These data suggest that distinguishing the two groups of isolates is difficult based upon the characterization criteria used. Significance and Impact of the StudyIn this study, antimicrobial resistance phenotype, virulence gene profile and genetic relatedness of Enterococcus cecorum isolated from diseased broiler chickens and poultry carcass rinsates were determined. The majority of isolates from both groups were multidrug resistant and harboured few virulence determinants. Results from this study suggest that clinical Ent.cecorum and Ent.cecorum from poultry carcass rinsates may share a common genetic background; clinical Ent.cecorum possess virulence determinants that have not been previously described for this species. Elucidation of those unknown virulence determinants is important for understanding the pathogenesis of Ent.cecorum infections in poultry. C1 [Jackson, C. R.; Frye, J. G.; Barrett, J. B.; Hiott, L. M.; Woodley, T. A.] USDA ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, Russell Res Ctr, Athens, GA 30613 USA. [Kariyawasam, S.] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Borst, L. B.] N Carolina State Univ, Coll Vet Med, Dept Populat Hlth & Pathobiol, Raleigh, NC USA. RP Jackson, CR (reprint author), ARS, USDA, Bacterial Epidemiol & Antimicrobial Resistance Re, Richard B Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM charlene.jackson@ars.usda.gov OI Frye, Jonathan/0000-0002-8500-3395 NR 33 TC 4 Z9 4 U1 2 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD FEB PY 2015 VL 60 IS 2 BP 111 EP 119 DI 10.1111/lam.12374 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA2HA UT WOS:000348728200002 PM 25470229 ER PT J AU Palumbo, JD O'Keeffe, TL AF Palumbo, J. D. O'Keeffe, T. L. TI Detection and discrimination of four Aspergillus section Nigri species by PCR SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE Aspergillus carbonarius; Aspergillus niger; calmodulin; fumonisin; grapes; ochratoxin; soil; species identification ID REAL-TIME PCR; OCHRATOXIN-A; BLACK ASPERGILLI; CALIFORNIA RAISINS; GRAPES; STRAINS; NIGER; CARBONARIUS; CYTOTOXICITY; ASSAYS AB Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically are identified by DNA sequencing methods. We developed four PCR primers to distinguish between Aspergillus niger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis, based on species-conserved differences in the calmodulin gene sequence. PCR amplification from total DNA using these primers was species specific; no amplification occurred from nontarget species DNA for each primer pair. Species-specific PCR could distinguish between species in mixed DNA templates, indicating a utility in determining culture uniformity of isolated Aspergillus strains. In addition, with these primer sets, each species could be detected in soil following mixed-species inoculation with Aspergillus spores. This indicates that PCR with these species-specific primers may be useful in determining the distribution of Aspergillus species in environmental samples without the need for species identification from isolated strains, as well as detecting species that may be infrequently isolated by culture-based methods. Significance and Impact of the StudyAspergillus section Nigri includes several species that produce ochratoxin A and fumonisin, two mycotoxins impacting food safety and human health. However, identification of these species is difficult without DNA sequencing. Using species-specific calmodulin gene sequences, we designed PCR primers to distinguish among Aspergillusniger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis as accurately and more quickly than by sequence analysis. We demonstrated that these PCR methods can be used to detect and identify each species within mixed populations without fungal isolation. This will facilitate assessing at-risk foods for potential mycotoxin contamination by these species. C1 [Palumbo, J. D.; O'Keeffe, T. L.] ARS, Foodborne Toxin Detect & Prevent Res Unit, USDA, Albany, CA 94710 USA. RP Palumbo, JD (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM jeffrey.palumbo@ars.usda.gov FU U.S. Department of Agriculture Agricultural Research Service CRIS [5325-42000-038-00] FX This work was supported by U.S. Department of Agriculture Agricultural Research Service CRIS project 5325-42000-038-00. NR 28 TC 2 Z9 2 U1 3 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD FEB PY 2015 VL 60 IS 2 BP 188 EP 195 DI 10.1111/lam.12358 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA2HA UT WOS:000348728200013 PM 25384730 ER PT J AU Tillman, GE Simmons, M Wasilenko, JL Narang, N Cray, WC Bodeis-Jones, S Martin, G Gaines, S Seal, BS AF Tillman, G. E. Simmons, M. Wasilenko, J. L. Narang, N. Cray, W. C. Bodeis-Jones, S. Martin, G. Gaines, S. Seal, B. S. TI Development of a Real-Time PCR for Escherichia coli based on gadE, an acid response regulatory gene SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE acid resistance; E; coli; gadE; Non-O157 STEC; real-time PCR ID GROUND-BEEF; EVOLUTIONARY GENETICS; SHIGELLA-SONNEI; RESISTANCE; STRAINS; WATER; YHIE; STX2 AB Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia coli from an agar plate. GadE (gadE) directly regulates the glutamate-dependent acid response system (GDAR) in E.coli and is responsible for survival of at pH 2. Based on gene sequence data, a real-time PCR assay targeting gadE was developed for this purpose. Seventy bacterial isolates recovered from ground beef enrichments and 714 isolates from caecal contents were identified biochemically and tested with the real-time PCR assay developed in this study. The PCR assay and the biochemical identification had 100% agreement on the tested isolates. The gadE real-time PCR assay was demonstrated in this study to be an inexpensive, reliable method for confirming E.coli colonies within 15h from an agar plate, thereby saving on final identification time. Significance and Impact of the StudyThere is always a need for rapid, inexpensive tests to identify bacterial cultures of public health concern at the species level. Recently, the numbers of tests performed to identify a culture as Escherichia coli have increased as regulations on pathogenic E.coli have increased as well as interest in the antibiotic resistance patterns of these organisms. The real-time PCR assay developed in this study provides a rapid, cost-effective way to identify E.coli from an agar plate without biochemical identification. This has importance for public health agencies in terms of analyst time and resource utilization. C1 [Tillman, G. E.; Simmons, M.; Wasilenko, J. L.; Narang, N.; Cray, W. C.] US Food Safety & Inspect Serv, USDA, Eastern Lab, Outbreaks Sect, Athens, GA USA. [Bodeis-Jones, S.; Martin, G.; Gaines, S.] US FDA, Ctr Vet Med, Res Off, Laurel, MD USA. [Seal, B. S.] ARS, USDA, Poultry Microbiol Safety Res Unit, Athens, GA USA. RP Tillman, GE (reprint author), USDA FSIS OPHS Eastern Lab, 950 Coll Stn Rd, Athens, GA 30605 USA. EM glenn.tillman@fsis.usda.gov NR 30 TC 0 Z9 0 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD FEB PY 2015 VL 60 IS 2 BP 196 EP 202 DI 10.1111/lam.12359 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA CA2HA UT WOS:000348728200014 PM 25384850 ER PT J AU Leisso, RS Buchanan, DA Lee, J Mattheis, JP Sater, C Hanrahan, I Watkins, CB Gapper, N Johnston, JW Schaffer, RJ Hertog, MLATM Nicolai, BM Rudell, DR AF Leisso, Rachel S. Buchanan, David A. Lee, Jinwook Mattheis, James P. Sater, Chris Hanrahan, Ines Watkins, Christopher B. Gapper, Nigel Johnston, Jason W. Schaffer, Robert J. Hertog, Maarten L. A. T. M. Nicolai, Bart M. Rudell, David R. TI Chilling-related cell damage of apple (Malus x domestica Borkh.) fruit cortical tissue impacts antioxidant, lipid and phenolic metabolism SO PHYSIOLOGIA PLANTARUM LA English DT Article ID CONTROLLED-ATMOSPHERE STORAGE; GRANNY-SMITH APPLES; SUPERFICIAL SCALD; HONEYCRISP APPLES; SOFT SCALD; OXIDATIVE STRESS; PLASMA-MEMBRANE; ABIOTIC STRESS; PLANTS; TEMPERATURE AB Soggy breakdown' (SB) is an internal flesh disorder of Honeycrisp' apple (Malus x domestica Borkh.) fruit that occurs during low temperature storage. The disorder is a chilling injury (CI) in which visible symptoms typically appear after several weeks of storage, but information about the underlying metabolism associated with its induction and development is lacking. The metabolic profile of flesh tissue from wholly healthy fruit and brown and healthy tissues from fruit with SB was characterized using gas chromatography-mass spectrometry (GC-MS) and liquid chromatograph-mass spectrometry (LC-MS). Partial least squares discriminant analysis (PLS-DA) and correlation networks revealed correlation among ester volatile compounds by composition and differences in phytosterol, phenolic and putative triacylglycerides (TAGs) metabolism among the tissues. anova-simultaneous component analysis (ASCA) was used to test the significance of metabolic changes linked with tissue health status. ASCA-significant components included antioxidant compounds, TAGs, and phytosterol conjugates. Relative to entirely healthy tissues, elevated metabolite levels in symptomatic tissue included -amino butyric acid, glycerol, sitosteryl (6-O-palmitoyl) -d-glucoside and sitosteryl (6-O-stearate) -d-glucoside, and TAGs containing combinations of 16:0, 18:3, 18:2 and 18:1 fatty acids. Reduced metabolite levels in SB tissue included 5-caffeoyl quinate, -carotene, catechin, epicatechin, -tocopherol, violaxanthin and sitosteryl -d glucoside. Pathway analysis indicated aspects of primary metabolism differed according to tissue condition, although differences in metabolites involved were more subtle than those of some secondary metabolites. The results implicate oxidative stress and membrane disruption processes in SB development and constitute a diagnostic metabolic profile for the disorder. C1 [Leisso, Rachel S.; Buchanan, David A.; Lee, Jinwook; Mattheis, James P.; Sater, Chris; Rudell, David R.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Leisso, Rachel S.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Hanrahan, Ines] Washington Tree Fruit Res Commiss, Wenatchee, WA USA. [Watkins, Christopher B.; Gapper, Nigel] Cornell Univ, Dept Hort, Ithaca, NY USA. [Gapper, Nigel] Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA. [Johnston, Jason W.; Schaffer, Robert J.] New Zealand Inst Plant & Food Res Ltd, Auckland, New Zealand. [Hertog, Maarten L. A. T. M.; Nicolai, Bart M.] Katholieke Univ Leuven, BIOSYST MeBioS, B-3001 Heverlee, Belgium. RP Rudell, DR (reprint author), USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM David.Rudell@ars.usda.gov RI nicolai, bart/L-6129-2013; Johnston, Jason/D-5947-2016 OI nicolai, bart/0000-0001-5267-1920; Johnston, Jason/0000-0002-6326-4885 NR 66 TC 9 Z9 9 U1 7 U2 66 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD FEB PY 2015 VL 153 IS 2 BP 204 EP 220 DI 10.1111/ppl.12244 PG 17 WC Plant Sciences SC Plant Sciences GA CA2ES UT WOS:000348722400002 PM 24944043 ER PT J AU Barnaby, JY Fleisher, D Reddy, V Sicher, R AF Barnaby, Jinyoung Y. Fleisher, David Reddy, Vangimalla Sicher, Richard TI Combined effects of CO2 enrichment, diurnal light levels and water stress on foliar metabolites of potato plants grown in naturally sunlit controlled environment chambers SO PHYSIOLOGIA PLANTARUM LA English DT Article ID SOLANUM-TUBEROSUM; ARABIDOPSIS-THALIANA; OSMOTIC ADJUSTMENT; DROUGHT TOLERANCE; ELEVATED CO2; AMINO-ACIDS; LEAVES; RESPONSES; GENES; ACCUMULATION AB Experiments were conducted in outdoor, naturally sunlit, soil-plant-atmosphere research (SPAR) chambers using plants grown in pots. Drought treatments were imposed on potato plants (Solanum tuberosum cv. Kennebec) beginning 10 days after tuber initiation. A total of 23 out of 37 foliar metabolites were affected by drought when measured 11 days after initiating water stress treatments. Compounds that accumulated in response to drought were hexoses, polyols, branched chain amino acids (BCAAs) and aromatic amino acids, such as proline. Conversely, leaf starch, alanine, aspartate and several organic acids involved in respiratory metabolism decreased with drought. Depending upon harvest date, a maximum of 12 and 17 foliar metabolites also responded to either CO2 enrichment or diurnal treatments, respectively. In addition, about 20% of the measured metabolites in potato leaflets were simultaneously affected by drought, CO2 enrichment and diurnal factors combined. This group contained BCAAs, hexoses, leaf starch and malate. Polyols and proline accumulated in response to water stress but did not vary diurnally. Water stress also amplified diurnal variations of hexoses and starch in comparison to control samples. Consequently, specific drought responsive metabolites in potato leaflets were dramatically affected by daily changes of photosynthetic carbon metabolism. C1 [Barnaby, Jinyoung Y.; Fleisher, David; Reddy, Vangimalla; Sicher, Richard] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. RP Barnaby, JY (reprint author), USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. EM jinyoung.barnaby@ars.usda.gov NR 41 TC 5 Z9 5 U1 3 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD FEB PY 2015 VL 153 IS 2 BP 243 EP 252 DI 10.1111/ppl.12238 PG 10 WC Plant Sciences SC Plant Sciences GA CA2ES UT WOS:000348722400005 PM 24888746 ER PT J AU Potvin, LR Kane, ES Chimner, RA Kolka, RK Lilleskov, EA AF Potvin, Lynette R. Kane, Evan S. Chimner, Rodney A. Kolka, Randall K. Lilleskov, Erik A. TI Effects of water table position and plant functional group on plant community, aboveground production, and peat properties in a peatland mesocosm experiment (PEATcosm) SO PLANT AND SOIL LA English DT Article DE Peatland; Plant functional group; Water table; Sphagnum; Ericaceae; Sedge ID CLIMATE-CHANGE; BOREAL PEATLANDS; SPHAGNUM MOSSES; LEVEL DRAWDOWN; CO2 EXCHANGE; ORGANIC-MATTER; PINE MIRES; BOG; GROWTH; VEGETATION AB Our objective was to assess the impacts of water table position and plant functional type on peat structure, plant community composition and aboveground plant production. We initiated a full factorial experiment with 2 water table (WT) treatments (high and low) and 3 plant functional groups (PFG: sedge, Ericaceae, sedge and Ericaceae- unmanipulated) in twenty-four 1 m(3) intact peatland mesocosms. We measured vegetation cover, aboveground plant production, and peat subsidence to analyze interactive PFG and WT effects. Sphagnum rubellum cover increased under high WT, while Polytrichum cover increased with low WT and in sedge only PFGs. Sphagnum production was greatest with high WT, while vascular plant production was greater in low WT treatments. There was an interactive WT x PFG effect on Ericaceae production. Lowered WT resulted in significant peat surface change and increased subsidence. There were significant PFG and WT effects on net peat accumulation, with the lowest rates of accumulation, high and low WT, in sedge only PFGs. The shift in water balance leading to lowered water table position predicted with changing climate could impact plant community composition and production, and would likely result in the subsidence of peat. C1 [Potvin, Lynette R.; Kane, Evan S.; Lilleskov, Erik A.] US Forest Serv, USDA, No Res Stn, Houghton, MI 49931 USA. [Kane, Evan S.; Chimner, Rodney A.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Kolka, Randall K.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Potvin, LR (reprint author), US Forest Serv, USDA, No Res Stn, Houghton, MI 49931 USA. EM lrpotvin@fs.fed.us; eskane@mtu.edu; rchimner@mtu.edu; rkolka@fs.fed.us; elilleskov@fs.fed.us OI Potvin, Lynette/0000-0001-5029-6266 FU USDA Forest Service Northern Research Station Climate Change Program; National Science Foundation [DEB-1146149] FX This work was supported primarily by the USDA Forest Service Northern Research Station Climate Change Program and the National Science Foundation (DEB-1146149). The authors would like to thank Todd Ontl and L. Jamie Lamit, as well as two anonymous reviewers, for their helpful comments and suggestions. This work would not have been possible without the help of countless student technicians, including Kayla Griffith, Eryn Grupido and Jesse Barta. NR 88 TC 6 Z9 6 U1 10 U2 50 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD FEB PY 2015 VL 387 IS 1-2 BP 277 EP 294 DI 10.1007/s11104-014-2301-8 PG 18 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AZ6GT UT WOS:000348318100020 ER PT J AU Wubben, MJ Callahan, FE Velten, J Burke, JJ Jenkins, JN AF Wubben, Martin J. Callahan, Franklin E. Velten, Jeff Burke, John J. Jenkins, Johnie N. TI Overexpression of MIC-3 indicates a direct role for the MIC gene family in mediating Upland cotton (Gossypium hirsutum) resistance to root-knot nematode (Meloidogyne incognita) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID PLANT-PARASITIC NEMATODES; SSR MARKERS; L.; PATHOGENESIS; EXPRESSION; CHROMOSOMES; PENETRATION; INFECTION AB Transgene-based analysis of the MIC-3 gene provides the first report of a cotton gene having a direct role in mediating cotton resistance to root-knot nematode. Major quantitative trait loci have been mapped to Upland cotton (Gossypium hirsutum L.) chromosomes 11 and 14 that govern the highly resistant phenotype in response to infection by root-knot nematode (RKN; Meloidogyne incognita); however, nearly nothing is known regarding the underlying molecular determinants of this RKN-resistant phenotype. Multiple lines of circumstantial evidence have strongly suggested that the MIC (Meloidogyne Induced Cotton) gene family plays an integral role in mediating cotton resistance to RKN. In this report, we demonstrate that overexpression of MIC-3 in the RKN-susceptible genetic background Coker 312 reduces RKN egg production by ca. 60-75 % compared to non-transgenic controls and transgene-null sibling lines. MIC-3 transcript and protein overexpression were confirmed in root tissues of multiple independent transgenic lines with each line showing a similar level of increased resistance to RKN. In contrast to RKN fecundity, transgenic lines showed RKN-induced root galling similar to the susceptible controls. In addition, we determined that this effect of MIC-3 overexpression was specific to RKN as no effect was observed on reniform nematode (Rotylenchulus reniformis) reproduction. Transgenic lines did not show obvious alterations in growth, morphology, flowering, or fiber quality traits. Gene expression analyses showed that MIC-3 transcript levels in uninfected transgenic roots exceeded levels observed in RKN-infected roots of naturally resistant plants and that overexpression did not alter the regulation of native MIC genes in the genome. These results are the first report describing a direct role for a specific gene family in mediating cotton resistance to a plant-parasitic nematode. C1 [Wubben, Martin J.; Callahan, Franklin E.; Jenkins, Johnie N.] ARS, Genet & Precis Agr Res Unit, Crop Sci Res Lab, USDA, Mississippi State, MS 39762 USA. [Velten, Jeff; Burke, John J.] ARS, Cropping Syst Res Lab, USDA, Lubbock, TX USA. RP Wubben, MJ (reprint author), ARS, Genet & Precis Agr Res Unit, Crop Sci Res Lab, USDA, 810 Highway 12 East, Mississippi State, MS 39762 USA. EM martin.wubben@ars.usda.gov NR 28 TC 4 Z9 4 U1 0 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2015 VL 128 IS 2 BP 199 EP 209 DI 10.1007/s00122-014-2421-9 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AZ8EL UT WOS:000348447600002 PM 25376794 ER PT J AU Petersen, S Lyerly, JH Worthington, ML Parks, WR Cowger, C Marshall, DS Brown-Guedira, G Murphy, JP AF Petersen, Stine Lyerly, Jeanette H. Worthington, Margaret L. Parks, Wesley R. Cowger, Christina Marshall, David S. Brown-Guedira, Gina Murphy, J. Paul TI Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID LEAF RUST RESISTANCE; TRITICUM-AESTIVUM L.; STEM RUST; CONFERRING RESISTANCE; HEXAPLOID WHEAT; POLYPLOID WHEAT; BREAD WHEAT; MOLECULAR CHARACTERIZATION; CHROMOSOMAL LOCATION; MARKER ANALYSIS AB A powdery mildew resistance gene was introgressed from Aegilops speltoides into winter wheat and mapped to chromosome 5BL. Closely linked markers will permit marker-assisted selection for the resistance gene. Powdery mildew of wheat (Triticum aestivum L.) is a major fungal disease in many areas of the world, caused by Blumeria graminis f. sp. tritici (Bgt). Host plant resistance is the preferred form of disease prevention because it is both economical and environmentally sound. Identification of new resistance sources and closely linked markers enable breeders to utilize these new sources in marker-assisted selection as well as in gene pyramiding. Aegilops speltoides (2n = 2x = 14, genome SS), has been a valuable disease resistance donor. The powdery mildew resistant wheat germplasm line NC09BGTS16 (NC-S16) was developed by backcrossing an Ae. speltoides accession, TAU829, to the susceptible soft red winter wheat cultivar 'Saluda'. NC-S16 was crossed to the susceptible cultivar 'Coker 68-15' to develop F-2:3 families for gene mapping. Greenhouse and field evaluations of these F-2:3 families indicated that a single gene, designated Pm53, conferred resistance to powdery mildew. Bulked segregant analysis showed that multiple simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers specific to chromosome 5BL segregated with the resistance gene. The gene was flanked by markers Xgwm499, Xwmc759, IWA6024 (0.7 cM proximal) and IWA2454 (1.8 cM distal). Pm36, derived from a different wild wheat relative (T. turgidum var. dicoccoides), had previously been mapped to chromosome 5BL in a durum wheat line. Detached leaf tests revealed that NC-S16 and a genotype carrying Pm36 differed in their responses to each of three Bgt isolates. Pm53 therefore appears to be a new source of powdery mildew resistance. C1 [Petersen, Stine; Lyerly, Jeanette H.; Worthington, Margaret L.; Murphy, J. Paul] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Parks, Wesley R.; Cowger, Christina; Marshall, David S.; Brown-Guedira, Gina] N Carolina State Univ, USDA, ARS, Raleigh, NC 27695 USA. RP Petersen, S (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM speters@ncsu.edu OI Worthington, Margaret/0000-0001-8019-165X FU North Carolina Small Grain Growers Association FX The research was supported by the North Carolina Small Grain Growers Association. Thanks to Jon Raupp at Kansas State University Wheat Genetics Resource Center for his help in obtaining seeds of Chinese Spring deletion bin lines. We gratefully acknowledge Prof. Bob McIntosh for his guidance and review of this paper. NR 59 TC 11 Z9 15 U1 5 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2015 VL 128 IS 2 BP 303 EP 312 DI 10.1007/s00122-014-2430-8 PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AZ8EL UT WOS:000348447600011 PM 25425170 ER PT J AU Ostlie, M Haley, SD Anderson, V Shaner, D Manmathan, H Beil, C Westra, P AF Ostlie, Michael Haley, Scott D. Anderson, Victoria Shaner, Dale Manmathan, Harish Beil, Craig Westra, Phillip TI Development and characterization of mutant winter wheat (Triticum aestivum L.) accessions resistant to the herbicide quizalofop SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID ACETYL-COA CARBOXYLASE; COENZYME-A CARBOXYLASE; AVENA-FATUA BIOTYPES; WILD OAT; CARBOXYLTRANSFERASE DOMAIN; ACETOLACTATE SYNTHASE; INHIBITING HERBICIDES; MUTATIONS; TOLERANCE; MECHANISM AB New herbicide resistance traits in wheat were produced through the use of induced mutagenesis. While herbicide-resistant crops have become common in many agricultural systems, wheat has seen few introductions of herbicide resistance traits. A population of Hatcher winter wheat treated with ethyl methanesulfonate was screened with quizalofop to identify herbicide-resistant plants. Initial testing identified plants that survived multiple quizalofop applications. A series of experiments were designed to characterize this trait. In greenhouse studies the mutants exhibited high levels of quizalofop resistance compared to non-mutant wheat. Sequencing ACC1 revealed a novel missense mutation causing an alanine to valine change at position 2004 (Alopecurus myosuroides reference sequence). Plants carrying single mutations in wheat's three genomes (A, B, D) were identified. Acetyl co-enzyme A carboxylase in resistant plants was 4- to 10-fold more tolerant to quizalofop. Populations of segregating backcross progenies were developed by crossing each of the three individual mutants with wild-type wheat. Experiments conducted with these populations confirmed largely normal segregation, with each mutant allele conferring an additive level of resistance. Further tests showed that the A genome mutation conferred the greatest resistance and the B genome mutation conferred the least resistance to quizalofop. The non-transgenic herbicide resistance trait identified will enhance weed control strategies in wheat. C1 [Ostlie, Michael] N Dakota State Univ, Carrington Res Extens Ctr, Carrington, ND 58421 USA. [Haley, Scott D.; Anderson, Victoria; Manmathan, Harish; Beil, Craig] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Shaner, Dale] ARS, USDA, Ft Collins, CO 80523 USA. [Westra, Phillip] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. RP Ostlie, M (reprint author), N Dakota State Univ, Carrington Res Extens Ctr, Carrington, ND 58421 USA. EM mike.ostlie@ndsu.edu RI Haley, Scott/C-1228-2013 FU Colorado Wheat Administrative Committee; Colorado Wheat Research Foundation FX This research was supported by the Colorado Wheat Administrative Committee and the Colorado Wheat Research Foundation. Thanks to Galen Brunk who provided guidance in the initiation of mutagenesis and the ACCase assay. NR 31 TC 1 Z9 1 U1 1 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2015 VL 128 IS 2 BP 343 EP 351 DI 10.1007/s00122-014-2434-4 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AZ8EL UT WOS:000348447600014 PM 25432092 ER PT J AU Dhanapal, AP Ray, JD Singh, SK Hoyos-Villegas, V Smith, JR Purcell, LC King, CA Cregan, PB Song, QJ Fritschi, FB AF Dhanapal, Arun Prabhu Ray, Jeffery D. Singh, Shardendu K. Hoyos-Villegas, Valerio Smith, James R. Purcell, Larry C. King, C. Andy Cregan, Perry B. Song, Qijian Fritschi, Felix B. TI Genome-wide association study (GWAS) of carbon isotope ratio (delta C-13) in diverse soybean [Glycine max (L.) Merr.] genotypes (vol 128, pg 73, 2015) SO THEORETICAL AND APPLIED GENETICS LA English DT Correction C1 [Dhanapal, Arun Prabhu; Fritschi, Felix B.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Ray, Jeffery D.; Smith, James R.] ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38776 USA. [Singh, Shardendu K.] ARS, Crop Syst & Global Change Lab, USDA, Beltsville, MD USA. [Hoyos-Villegas, Valerio] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Purcell, Larry C.; King, C. Andy] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Cregan, Perry B.; Song, Qijian] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Fritschi, FB (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM fritschif@missouri.edu RI Dhanapal, Arun Prabhu/F-5030-2012 OI Dhanapal, Arun Prabhu/0000-0002-9686-7260 NR 1 TC 0 Z9 0 U1 4 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD FEB PY 2015 VL 128 IS 2 BP 375 EP 376 DI 10.1007/s00122-014-2450-4 PG 2 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AZ8EL UT WOS:000348447600017 PM 25575838 ER PT J AU Ranger, CM Tobin, PC Reding, ME AF Ranger, Christopher M. Tobin, Patrick C. Reding, Michael E. TI Ubiquitous volatile compound facilitates efficient host location by a non-native ambrosia beetle SO BIOLOGICAL INVASIONS LA English DT Article DE Xylosandrus germanus; Ambrosia beetles; Ethanol; Colonization dynamics ID ETHANOL-BAITED TRAPS; COLEOPTERA-CURCULIONIDAE; BARK BEETLES; XYLOSANDRUS-GERMANUS; UNITED-STATES; ORNAMENTAL NURSERIES; CHESTNUT BORER; FREEZE DAMAGE; SCOLYTINAE; CLIMATE AB Xylosandrus germanus (Blandford), a species native to Asia but currently invading North American and European forests, exploits living, but weakened trees. In response to many sources of stress, trees emit ethanol, which represents an important host-location cue for X. germanus. Because stressed trees can be spatially and temporally variable over a landscape, we assessed the role of olfaction in aiding X. germanus to efficiently locate vulnerable trees during natural dispersal. We conducted a series of experiments and observed that attacks occurred on trees baited with ethanol, but immediately ceased upon removal of the ethanol cue. X. germanus also efficiently located and attacked ethanol-injected trees, but rarely landed on adjacent trees not emitting ethanol, and never attacked these neighboring trees. A spatial analysis of trees attacked by ambrosia beetles within diverse landscapes revealed that only certain host species or cultivars, and only certain individuals within these host species or cultivars, were attacked; ethanol was also detected in the attacked trees, but not in non-attacked trees. Thus, X. germanus uses an efficient olfactory mechanism while orienting among perceived non-hosts to specifically locate trees associated with ethanol. Combined with other attributes, we propose that the remarkable efficiency by which this non-native ambrosia beetle uses volatile cues to locate specific vulnerable hosts across a diverse landscape aids its successful establishment and population spread. C1 [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Hort Insects Res Lab, Wooster, OH 44691 USA. [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Tobin, Patrick C.] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. RP Ranger, CM (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Hort Insects Res Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM christopher.ranger@ars.usda.gov FU USDA Floriculture and Nursery Research Initiative [3607-22000-012-00D] FX This research was supported in part by funding from the USDA Floriculture and Nursery Research Initiative and base funds associated with ARS Research Project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine). We thank James Moyseenko, Jennifer Barnett, Betsy Anderson, and Leslie Morris for technical assistance. We also thank anonymous reviewers for useful comments. Mention of proprietary products or companies does not imply any endorsement or preferential treatment by the USDA-Agricultural Research Service, USDA-Forest Service, or The Ohio State University. NR 64 TC 8 Z9 8 U1 2 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD FEB PY 2015 VL 17 IS 2 BP 675 EP 686 DI 10.1007/s10530-014-0758-2 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AZ3TO UT WOS:000348149700013 ER PT J AU Lima, SL Blackwell, BF DeVault, TL Fernandez-Juricic, E AF Lima, Steven L. Blackwell, Bradley F. DeVault, Travis L. Fernandez-Juricic, Esteban TI Animal reactions to oncoming vehicles: a conceptual review SO BIOLOGICAL REVIEWS LA English DT Article DE animal-object collisions; animal-vehicle collisions; antipredator behaviour; avoidance; sensory; wildlife strikes ID BOTTLE-NOSED DOLPHINS; PREDATOR-PREY INTERACTIONS; US CIVIL AIRCRAFT; NEW-SOUTH-WALES; ANTIPREDATOR BEHAVIOR; ROAD MORTALITY; BIRD STRIKES; EYE SIZE; WILDLIFE MANAGEMENT; AVOIDANCE-BEHAVIOR AB Animal-vehicle collisions (AVCs) are a substantial problem in a human-dominated world, but little is known about what goes wrong, from the animal's perspective, when a collision occurs with an automobile, boat, or aircraft. Our goal is to provide insight into reactions of animals to oncoming vehicles when collisions might be imminent. Avoiding a collision requires successful vehicle detection, threat assessment, and evasive behaviour; failures can occur at any of these stages. Vehicle detection seems fairly straightforward in many cases, but depends critically on the sensory capabilities of a given species. Sensory mechanisms for detection of collisions (looming detectors) may be overwhelmed by vehicle speed. Distractions are a likely problem in vehicle detection, but have not been clearly demonstrated in any system beyond human pedestrians. Many animals likely perceive moving vehicles as non-threatening, and may generally be habituated to their presence. Slow or minimal threat assessment is thus a likely failure point in many AVCs, but this is not uniformly evident. Animals generally initiate evasive behaviour when a collision appears imminent, usually employing some aspect of native antipredator behaviour. Across taxa, animals exhibit a variety of behaviours when confronted with oncoming vehicles. Among marine mammals, right whales Eubalaena spp., manatees Trichechus spp., and dugongs Dugong dugon are fairly unresponsive to approaching vehicles, suggesting a problem in threat assessment. Others, such as dolphins Delphinidae, assess vehicle approach at distance. Little work has been conducted on the behavioural aspects of AVCs involving large mammals and automobiles, despite their prevalence. Available observations suggest that birds do not usually treat flying aircraft as a major threat, often allowing close approach before taking evasive action, as they might in response to natural predators. Inappropriate antipredator behaviour (often involving immobility) is a major source of AVCs in amphibians and terrestrial reptiles. Much behavioural work on AVCs remains to be done across a wide variety of taxa. Such work should provide broad phylogenetic generalizations regarding AVCs and insights into managing AVCs. C1 [Lima, Steven L.] Indiana State Univ, Dept Biol, Terre Haute, IN 47809 USA. [Blackwell, Bradley F.; DeVault, Travis L.] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Wildlife Serv,Ohio Field Stn, Sandusky, OH 44870 USA. [Fernandez-Juricic, Esteban] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. RP Fernandez-Juricic, E (reprint author), Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. EM efernan@purdue.edu FU U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center FX We thank Michael Gerringer for providing helpful comments on the manuscript and help in its preparation. Support for B.F.B., T.L.D., and E.F.-J. was provided by the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center. NR 190 TC 16 Z9 16 U1 16 U2 121 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-7931 EI 1469-185X J9 BIOL REV JI Biol. Rev. PD FEB PY 2015 VL 90 IS 1 BP 60 EP 76 DI 10.1111/brv.12093 PG 17 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AZ7RL UT WOS:000348415500004 PM 24661508 ER PT J AU Makarem, N Lin, Y Bandera, EV Jacques, PF Parekh, N AF Makarem, Nour Lin, Yong Bandera, Elisa V. Jacques, Paul F. Parekh, Niyati TI Concordance with World Cancer Research Fund/American Institute for Cancer Research (WCRF/AICR) guidelines for cancer prevention and obesity-related cancer risk in the Framingham Offspring cohort (1991-2008) SO CANCER CAUSES & CONTROL LA English DT Article DE Healthful behaviors; American Institute for Cancer Research; Cancer prevention guidelines; Cancer; Framingham Heart Study ID FOOD FREQUENCY QUESTIONNAIRE; PHYSICAL-ACTIVITY; WOMENS HEALTH; ADHERENCE; NUTRITION; RECOMMENDATIONS; STATISTICS; MORTALITY AB This prospective cohort study evaluates associations between healthful behaviors consistent with WCRF/AICR cancer prevention guidelines and obesity-related cancer risk, as a third of cancers are estimated to be preventable. The study sample consisted of adults from the Framingham Offspring cohort (n = 2,983). From 1991 to 2008, 480 incident doctor-diagnosed obesity-related cancers were identified. Data on diet, measured by a food frequency questionnaire, anthropometric measures, and self-reported physical activity, collected in 1991 was used to construct a 7-component score based on recommendations for body fatness, physical activity, foods that promote weight gain, plant foods, animal foods, alcohol, and food preservation, processing, and preparation. Multivariable Cox regression models were used to estimate associations between the computed score, its components, and subcomponents in relation to obesity-related cancer risk. The overall score was not associated with obesity-related cancer risk after adjusting for age, sex, smoking, energy, and preexisting conditions (HR 0.94, 95 % CI 0.86-1.02). When score components were evaluated separately, for every unit increment in the alcohol score, there was 29 % lower risk of obesity-related cancers (HR 0.71, 95 % CI 0.51-0.99) and 49-71 % reduced risk of breast, prostate, and colorectal cancers. Every unit increment in the subcomponent score for non-starchy plant foods (fruits, vegetables, and legumes) among participants who consume starchy vegetables was associated with 66 % reduced risk of colorectal cancer (HR 0.44, 95 % CI 0.22-0.88). Lower alcohol consumption and a plant-based diet consistent with the cancer prevention guidelines were associated with reduced risk of obesity-related cancers in this population. C1 [Makarem, Nour; Parekh, Niyati] NYU, Steinhardt Sch, Dept Nutr Food Studies & Publ Hlth, New York, NY 10003 USA. [Lin, Yong; Bandera, Elisa V.] Rutgers State Univ, Rutgers Sch Publ Hlth, Piscataway, NJ 08854 USA. [Lin, Yong; Bandera, Elisa V.] Rutgers Canc Inst New Jersey, New Brunswick, NJ 08903 USA. [Jacques, Paul F.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Parekh, Niyati] NYU, Langone Sch Med, Dept Populat Hlth, New York, NY 10016 USA. RP Parekh, N (reprint author), NYU, Steinhardt Sch, Dept Nutr Food Studies & Publ Hlth, 411 LaFayette St,5th Floor,Room 542, New York, NY 10003 USA. EM niyati.parekh@nyu.edu RI Bandera, Elisa/M-4169-2014; OI Bandera, Elisa/0000-0002-8789-2755; parekh , niyati /0000-0002-1334-0528 FU American Cancer Society Research Scholar Grant [RSG-12-005-01-CNE]; National Heart, Lung, and Blood Institute (NHLBI); Boston University [N01-HC-25195]; ARS [53-3k06-5-10, 58-1950-9-001, 58-1950-4-401, 58-1950-7-707] FX This research was supported by the American Cancer Society Research Scholar Grant (#RSG-12-005-01-CNE) awarded to Niyati Parekh, PhD RD. The Framingham Heart Study is conducted and supported by the National Heart, Lung, and Blood Institute (NHLBI) in collaboration with Boston University (Contract No. N01-HC-25195). Funding support for the Framingham Food Frequency Questionnaire datasets was provided by ARS Contract #53-3k06-5-10, ARS Agreement #'s 58-1950-9-001, 58-1950-4-401, and 58-1950-7-707. This manuscript was not prepared in collaboration with investigators of the Framingham Heart Study and does not necessarily reflect the opinions or views of the Framingham Heart Study, Boston University, or NHLBI. NR 19 TC 10 Z9 10 U1 1 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0957-5243 EI 1573-7225 J9 CANCER CAUSE CONTROL JI Cancer Causes Control PD FEB PY 2015 VL 26 IS 2 BP 277 EP 286 DI 10.1007/s10552-014-0509-9 PG 10 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA AZ4IF UT WOS:000348184100012 PM 25559553 ER PT J AU Palik, BJ Haworth, BK David, AJ Kolka, RK AF Palik, Brian J. Haworth, Brooke K. David, Andrew J. Kolka, Randall K. TI Survival and growth of northern white-cedar and balsam fir seedlings in riparian management zones in northern Minnesota, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Deer browsing; Seedling survival; Growth; Riparian forest; Thuja occidentalis; Abies balsamea ID FORESTS; REGENERATION; DEER; MICHIGAN; WISCONSIN; DYNAMICS; CANADA; STANDS; MAINE AB Northern white-cedar (Thuja occidentalis) and balsam fir (Abies balsamea.) are co-occurring species in riparian forests of the western Great Lakes region. Throughout much of the region, northern white-cedar has been experiencing population declines due to herbivory by white-tailed deer (Odocoileus virginianus). Preferential browse on northern white-cedar removes seedlings and saplings, allowing balsam fir, as less-preferred browse species, to recruit into larger size classes and potentially out-compete northern white-cedar. There is great interest in restoring and sustaining northern white-cedar in riparian forests, but the factors that contribute to success or failure of established seedlings are not well understood. We used a riparian harvesting experiment in northern Minnesota, USA to study factors that encourage or deter survival and growth of planted northern white-cedar seedlings in riparian areas, relative to the response of balsam fir. The factors we manipulated experimentally included amount of overstory basal area (uncut or partially harvested), establishment microsite (mound, pit, slash), and browse (protected or not). Browse frequency was significantly higher on northern white-cedar than on balsam fir, particularly in the partially-harvested forest. Northern white cedar survival was similar to balsam fir when unprotected from browsing, but was higher when browsing was excluded. When protected, northern white-cedar survival approached 100% in both uncut and partially-cut forest on mound and slash microsites, whereas survival of balsam fir was significantly lower in uncut compared to partially-cut forest. Both species had low survival in pit microsites. Relative height growth rates were similar between the two species when protected from browsing, and both species had higher growth with partial-harvest. When unprotected from browsing, relative height growth of northern white-cedar was often negative, while height growth of balsam fir was only minimally reduced compared to the protected seedlings. The two species had similar relative diameter growth in the unprotected treatment, and growth of both species was reduced compared to growth when protected. When protected, northern white-cedar had a relative diameter growth advantage over balsam fir, particularly in the partial-harvest treatment. Our results suggest that a strategy to establish northern white-cedar in riparian forests may be to plant seedlings in uncut forest on raised microsites and protect these seedlings from browsing. After these seedlings are well-established, and perhaps balsam fir is intentionally eliminated, a partial-harvest may be used to release the northern white-cedar seedlings, while still maintaining pre-harvest browse protection. Published by Elsevier B.V. C1 [Palik, Brian J.; Kolka, Randall K.] USDA Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Haworth, Brooke K.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [David, Andrew J.] Univ Minnesota, Dept Forest Resources, North Cent Res & Outreach Ctr, Grand Rapids, MN 55744 USA. RP Palik, BJ (reprint author), USDA Forest Serv, No Res Stn, 1831 Highway 169 East, Grand Rapids, MN 55744 USA. EM bpalik@fs.fed.us; bkhaworth@msn.com; adavid@umn.edu; rkolka@fs.fed.us FU USDA Forest Service Northern Research Station; University of Minnesota Department of Forest Resources; Minnesota Environment and Natural Resources Trust Fund FX Funding for this study was provided by the USDA Forest Service Northern Research Station, the University of Minnesota Department of Forest Resources, and the Minnesota Environment and Natural Resources Trust Fund. We thank Sandy Weisberg and Christopher Chizinski, who were instrumental with the experimental design and statistical analysis, and Leah Evison, Jeff Couture, Donna Olson and Beth Gaede for field assistance. NR 31 TC 1 Z9 1 U1 5 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2015 VL 337 BP 20 EP 27 DI 10.1016/j.foreco.2014.10.033 PG 8 WC Forestry SC Forestry GA AZ2TC UT WOS:000348084200003 ER PT J AU Gahagan, A Giardina, CP King, JS Binkley, D Pregitzer, KS Burton, AJ AF Gahagan, Adam Giardina, Christian P. King, John S. Binkley, Dan Pregitzer, Kurt S. Burton, Andrew J. TI Carbon fluxes, storage and harvest removals through 60 years of stand development in red pine plantations and mixed hardwood stands in Northern Michigan, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Ecosystem carbon; Litterfall; Belowground; Productivity; Total belowground carbon flux ID SOIL ORGANIC-CARBON; UPLAND FOREST ECOSYSTEMS; NET PRIMARY PRODUCTION; BELOW-GROUND BIOMASS; COARSE WOODY DEBRIS; SUGAR MAPLE FORESTS; SPATIAL VARIABILITY; NITROGEN DYNAMICS; CENTRAL WISCONSIN; ROOT RESPIRATION AB The storage and flow of carbon (C) into and out of forests can differ under the influence of dominant tree species because of species-based variation in C production, decomposition, retention, and harvest-based export. Following abandonment of agricultural activities in the first half of the 20th century, many landscapes of the Great Lakes region (USA) were planted to red pine (Pinus resinosa) or naturally regenerated to northern hardwood species including sugar maple (Acer saccharum), red oak (Quercus rubra) and red maple (Acer rubrum). We located eight pairs of adjacent, similarly aged (similar to 60 yr) stands of planted red pine and naturally regenerated hardwood forests on previous agricultural fields. We found that the hardwood forests stored more C than pine stands (255 vs. 201 Mg C ha(-1)), with both storing substantially more than an adjacent area maintained as pasture (107 Mg C ha(-1)). The greater accumulation of C in the hardwood stands occurred mostly in living biomass. No significant differences for soil C (to 1 m depth) were found between forest types, despite significantly higher belowground inputs and above-ground litterfall in hardwood stands. Notably, both forest types had about 18% more soil C than the pasture, with O horizon C accounting for about one-third of the increase under trees. Forest type had no significant effect on estimated amount of exported C despite fairly large differences in projected end uses (solid wood products, land-fills, bioenergy). Using adjacent pasture as our baseline condition, we combined estimated on-site accumulation rates with estimates of exported C, and found that average total C sequestration rates were higher for hardwood (2.9 Mg C ha(-1) yr(-1)) than red pine plots (2.3 Mg C ha(-1) yr(-1)). The modeled potential contribution of exported C to these sequestration rate estimates did not differ between species, but the fate of modeled post-harvest off-site C may exert a large influence on sequestration rate estimates depending on actual displacement actions, including product longevity. These results show that tree species selection has the potential to impact C sequestration rates but effects vary by ecosystem component and could not be predicted from previous species effects studies. (C) 2014 Published by Elsevier B.V. C1 [Gahagan, Adam; Burton, Andrew J.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Ecosyst Sci Ctr, Houghton, MI 49931 USA. [Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, USDA, Pacific Southwest Res Stn, Hilo, HI 96720 USA. [King, John S.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Binkley, Dan] Colorado State Univ, Dept Forest Rangeland & Watershed Stewardship, Ft Collins, CO 80523 USA. [Pregitzer, Kurt S.] Univ Idaho, Coll Nat Resources, Moscow, ID 83844 USA. RP Giardina, CP (reprint author), US Forest Serv, Inst Pacific Isl Forestry, USDA, PSW Res Stn, 60 Nowelo St, Hilo, HI 96720 USA. EM cgiardina@fs.fed.us RI Giardina, Christian/C-3120-2011 OI Giardina, Christian/0000-0002-3431-5073 FU National Council for Air and Stream Improvement, Inc. (NCASI); MWV Corporation; USDA Forest Service's Northern and Pacific Southwest Research Stations; Michigan Technological University FX We thank our financial supporters: National Council for Air and Stream Improvement, Inc. (NCASI), MWV Corporation, the Agenda 2020 Program through the USDA Forest Service's Northern and Pacific Southwest Research Stations, and Michigan Technological University. We also thank John Johnson at MWV who provided access to and background information on the field sites. We thank Drs. Alex Friend, Erik Lilleskov and Casey Huckins for earlier reviews of this manuscript. We appreciate the help of our field crew, Melissa and Matt Porter, Gordon Hart, Noah Karberg, Christa Luokkala, Chad Fate, John Durocher, Ron Peryam and Maria Janowiak, without whom this project would not have been possible. We would also thank Zach and Beth Reusch for soil analysis, and Cinzia Fissore for guidance through the soil incubation process. NR 81 TC 4 Z9 4 U1 4 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2015 VL 337 BP 88 EP 97 DI 10.1016/j.foreco.2014.10.037 PG 10 WC Forestry SC Forestry GA AZ2TC UT WOS:000348084200010 ER PT J AU Fowler, ZK Adams, MB Peterjohn, WT AF Fowler, Zachariah K. Adams, Mary Beth Peterjohn, William T. TI Will more nitrogen enhance carbon storage in young forest stands in central Appalachia? SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Nitrogen deposition; Carbon storage; Regenerating forests; Tree biomass; Temperate deciduous forest; Fernow Experimental Forest ID TERRESTRIAL ECOSYSTEMS; UNITED-STATES; N DEPOSITION; NORTHERN-HEMISPHERE; TEMPERATE FORESTS; NUTRIENT DYNAMICS; SPECIES RICHNESS; HARDWOOD FORESTS; ORGANIC-MATTER; BOREAL FORESTS AB Many temperate deciduous forests in the Eastern US are secondary, regrowing forests and have experienced decades of elevated inputs of acidic compounds and biologically available nitrogen (N) from the atmosphere. These young forests play an important role in the global carbon (C) cycle as C sinks, and it is possible that acidic deposition will influence the strength and longevity of this sink. We used the Fernow Experimental Forest Long Term Soil Productivity (LTSP) experiment near Parsons, WV to evaluate how 13 years of experimental N additions has affected ecosystem C storage and stand level dynamics in a young temperate deciduous forest. Specifically we examined whether N additions: (1) directly increased aboveground growth of regenerating trees but did so in a way that was independent of the indirect effects of soil acidification; (2) directly (independent of acidification effects) increased forest floor and soil C pools, and decreased the fine root C pool; and (3) lowered stand density and diversity. We also tested whether tree species were differentially affected by either N fertilization or soil acidification. Thirteen years of ammonium sulfate additions to a regenerating deciduous forest stimulated C storage by 22% - even in a region with historically high levels of atmospheric N deposition. This response was driven primarily by a 27% increase in C storage in aboveground biomass and to a lesser extent by a 35% increase in C stored in the smaller forest floor pool. Despite the dominance of a single tree species (Prunus pensylvanica), the overall response may have been tempered by reductions in stand density and was only detectable when the changes in all species were included, rather than when only the changes in the dominant species were examined. Indirect acidification effects were found to increase C accumulation in the forest floor and decrease the number of different tree species. In the short-term it is likely that N deposition will stimulate forest growth and C storage in young temperate deciduous forests. However, given the differential responses observed for longer-lived tree species versus the positive response for short-lived species, it appears that the long-term effects of N deposition on C storage in temperate deciduous forests may be different than the short-term effects, and may even be negative. (C) 2014 Elsevier B.V. All rights reserved. C1 [Fowler, Zachariah K.; Peterjohn, William T.] W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA. [Adams, Mary Beth] US Forest Serv, USDA, No Res Stn, Parsons, WV 26287 USA. RP Fowler, ZK (reprint author), W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA. EM zkfowler@gmail.com FU National Science Foundation [DEB-0417678, DEB-1019522] FX For logistical support and supplemental data we thank the staff at the USDA Forest Service, Northeastern Forest Experiment Station, Parsons, WV. Summer interns Anthony Barker, Pat Cress, Caroline Cummins, Elizabeth Ervin, Cody Greer, Meghan Hatfield, Tyler Holliday, Natalie Johnson, Chris Kennedy, Anna Lloyd, Kirsten Maier, Ben McIlhenny, Chad Northcraft, Kaci Orlandi, Kenny Smith, Eric Winter, and Jawad Zafar helped with a significant amount of the field sampling and laboratory analyses. Funding for this research was provided by the Long Term Research in Environmental Biology (LTREB) program at the National Science Foundation (Grant Nos. DEB-0417678 and DEB-1019522). NR 75 TC 4 Z9 4 U1 3 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD FEB 1 PY 2015 VL 337 BP 144 EP 152 DI 10.1016/j.foreco.2014.10.023 PG 9 WC Forestry SC Forestry GA AZ2TC UT WOS:000348084200016 ER PT J AU Lamba, J Karthikeyan, KG Thompson, AM AF Lamba, Jasmeet Karthikeyan, K. G. Thompson, A. M. TI Apportionment of suspended sediment sources in an agricultural watershed using sediment fingerprinting SO GEODERMA LA English DT Article DE Sediment sources; Phosphorus; Sediment fingerprinting; Suspended sediment; Soil erosion; RUSLE 2 ID RIVER-BASINS; CATCHMENTS; PHOSPHORUS; USA; SCALE; UK; STREAMBANKS; MANAGEMENT; WISCONSIN; EROSION AB Sediment fingerprinting techniques can provide valuable information on sources of suspended sediment to facilitate effective targeting of best management practices. Research was conducted in the Pleasant Valley watershed (50 km(2)) in South Central Wisconsin to identify sources of suspended sediment during cropping season and snowmelt periods at a subwatershed scale. Results show that both stream banks and agriculture are important sources of suspended sediment. The contribution from agriculture and stream banks to suspended sediment at the watershed outlet ranged from 45 to 97% and from 3 to 47%, respectively. During periods of high sediment loading agriculture was the important source of suspended sediment at the majority of sites within this watershed, with the exception of snowmelt runoff, when stream banks were the dominant source of suspended sediment at the watershed outlet. The average annual erosion rates in croplands and pasture land-use determined from the Revised Universal Soil Loss Equation 2 (RUSLE 2) ranged from 0 to 0.00509 t m(-2) yr(-1), indicating significant variability among fields. Conservation practices in this watershed should be targeted to stream banks or agriculture (prioritize fields based on RUSLE 2 average annual erosion rates) depending on the dominant source of suspended sediment within a subwatershed. The results of this study show that both temporal and spatial variability in suspended sediment sources should be considered to develop management strategies and sampling only at the watershed outlet or capturing a few storms might not be sufficient to target locations for best management practices. (C) 2014 Elsevier B.V. All rights reserved. C1 [Lamba, Jasmeet; Karthikeyan, K. G.; Thompson, A. M.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. RP Lamba, J (reprint author), USDA ARS, Pasture Syst &Watershed Management Res Unit, Bldg 3702 Curtin Rd, University Pk, PA 16802 USA. EM jul59@psu.edu RI LAMBA, JASMEET/B-3878-2015 FU USDA NIFA award [2009-51130-06049]; Nature Conservancy; Wisconsin Department of Natural Resources FX Funding for this project was provided by the USDA NIFA award #2009-51130-06049, The Nature Conservancy, and the Wisconsin Department of Natural Resources. We thank John Panuska, Laura Good, Faith Fitzpatrick, and staff at Dane County Land Conservation Division, Dane County, Wisconsin for their assistance in this study. We also thank all the producers in this watershed for allowing soil sampling from their lands. NR 42 TC 9 Z9 9 U1 4 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD FEB PY 2015 VL 239 BP 25 EP 33 DI 10.1016/j.geoderma.2014.09.024 PG 9 WC Soil Science SC Agriculture GA AY7XA UT WOS:000347768000003 ER PT J AU Brungard, CW Boettinger, JL Duniway, MC Wills, SA Edwards, TC AF Brungard, Colby W. Boettinger, Janis L. Duniway, Michael C. Wills, Skye A. Edwards, Thomas C., Jr. TI Machine learning for predicting soil classes in three semi-arid landscapes SO GEODERMA LA English DT Article DE Digital soil mapping; Machine learning; Recursive feature elimination; Random forests; Brier score ID SPATIAL PREDICTION; CLASSIFICATION; VARIABLES; KNOWLEDGE; MODEL; IMAGE; AREAS; IRAN; MAP; AUSTRALIA AB Mapping the spatial distribution of soil taxonomic classes is important for informing soil use and management decisions. Digital soil mapping (DSM) can quantitatively predict the spatial distribution of soil taxonomic classes. Key components of DSM are the method and the set of environmental covariates used to predict soil classes. Machine learning is a general term for a broad set of statistical modeling techniques. Many different machine learning models have been applied in the literature and there are different approaches for selecting covariates for DSM. However, there is little guidance as to which, if any, machine learning model and covariate set might be optimal for predicting soil classes across different landscapes. Our objective was to compare multiple machine learning models and covariate sets for predicting soil taxonomic classes at three geographically distinct areas in the semi-arid western United States of America (southern New Mexico, southwestern Utah, and northeastern Wyoming). All three areas were the focus of digital soil mapping studies. Sampling sites at each study area were selected using conditioned Latin hypercube sampling (cLHS). We compared models that had been used in other DSM studies, including clustering algorithms, discriminant analysis, multinomial logistic regression, neural networks, tree based methods, and support vector machine classifiers. Tested machine learning models were divided into three groups based on model complexity: simple, moderate, and complex. We also compared environmental covariates derived from digital elevation models and Landsat imagery that were divided into three different sets: 1) covariates selected a priori by soil scientists familiar with each area and used as input into cLHS, 2) the covariates in set 1 plus 113 additional covariates, and 3) covariates selected using recursive feature elimination. Overall, complex models were consistently more accurate than simple or moderately complex models. Random forests (RF) using covariates selected via recursive feature elimination was consistently the most accurate, or was among the most accurate, classifiers between study areas and between covariate sets within each study area. We recommend that for soil taxonomic class prediction, complex models and covariates selected by recursive feature elimination be used. Overall classification accuracy in each study area was largely dependent upon the number of soil taxonomic classes and the frequency distribution of pedon observations between taxonomic classes. Individual subgroup class accuracy was generally dependent upon the number of soil pedon observations in each taxonomic class. The number of soil classes is related to the inherent variability of a given area. The imbalance of soil pedon observations between classes is likely related to cLHS. Imbalanced frequency distributions of soil pedon observations between classes must be addressed to improve model accuracy. Solutions include increasing the number of soil pedon observations in classes with few observations or decreasing the number of classes. Spatial predictions using the most accurate models generally agree with expected soil landscape relationships. Spatial prediction uncertainty was lowest in areas of relatively low relief for each study area. (C) 2014 Elsevier B.V. All rights reserved. C1 [Brungard, Colby W.; Boettinger, Janis L.] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. [Duniway, Michael C.] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT 84532 USA. [Wills, Skye A.] Nat Resources Conservat Serv, Natl Soil Survey Ctr, USDA, Lincoln, NE 68508 USA. [Edwards, Thomas C., Jr.] Utah State Univ, Dept Wildland Resources, Utah Cooperat Fish & Wildlife Res Unit, US Geol Survey, Logan, UT 84322 USA. RP Brungard, CW (reprint author), Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. EM envsoilco@gmail.com; janis.boettinger@usu.edu; mduniway@usgs.gov; skye.wills@lin.usda.gov; tedwards@usu.edu OI Duniway, Michael/0000-0002-9643-2785 FU USDI Bureau of Land Management (BLM) via Cooperative Ecosystem Study Unit (CESU) [L09AC15757]; Utah Agricultural Experiment Station (UAES), Utah State University FX This research was supported in large part by the USDI Bureau of Land Management (BLM) via Cooperative Ecosystem Study Unit (CESU) Agreement number L09AC15757 and the Utah Agricultural Experiment Station (UAES), Utah State University. Approved as UAES journal paper number 8655. We would like to thank Jeremiah Armentrout, Brook Fonnesbeck, and the Fort Bliss military base for providing the NM dataset. Brook Fonnesbeck, Mike Leno, Zamir Libohova, Shawn Nield and Amanda Preddice collected the WY data. We would like to thank Rob Gentilion for assisting with covariate development. We would also like to thank two anonymous reviewers for their insightful comments, which substantially improved this article. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 89 TC 10 Z9 10 U1 7 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD FEB PY 2015 VL 239 BP 68 EP 83 DI 10.1016/j.geoderma.2014.09.019 PG 16 WC Soil Science SC Agriculture GA AY7XA UT WOS:000347768000007 ER PT J AU Zhang, XC Zhang, GH Wei, X AF Zhang, X. C. (John) Zhang, G. H. Wei, X. TI How to make Cs-137 erosion estimation more useful: An uncertainty perspective SO GEODERMA LA English DT Article DE Cesium-137; Cs-137 conversion model; Soil erosion; Sediment tracer; Uncertainty analysis; Spatial variability ID SOIL-EROSION; CESIUM-137 MEASUREMENTS; SPATIAL VARIABILITY; UNSUPPORTED PB-210; ESTIMATE RATES; WATER EROSION; TILLAGE; REDISTRIBUTION; MODELS; INVENTORIES AB Although the Cs-137 technique has been widely used to provide quantitative soil redistribution estimates since 1980s, no systematic sensitivity and uncertainty analyses have been carried out to evaluate the conversion models. The objectives of this study are to (1) perform sophisticated sensitivity and uncertainty analyses for the three widely-used models to characterize the potential sources of uncertainty including spatial variations on both reference and measuring sites and (2) explore ways to minimize uncertainty and to improve soil redistribution estimation. The normalized sensitivity showed that soil redistribution estimates were extremely sensitive to Cs-137 reference and sample inventories, and less sensitive, to the same degree, to bulk density, tillage depth, and particle size correction factor. Uncertainty analysis showed that the spatial variabilities on both reference and measuring sites were predominant contributors to overall uncertainty of soil erosion estimation, followed by particle size correction factor P, with negligible contributions from bulk density and tillage depth, showing that close attention must be paid to Cs-137 spatial variability and factor P. In the presence of substantial random spatial variation in Cs-137 distribution, the Cs-137 technique is not suitable for estimating point soil redistribution rates as is widely perceived in the literature, because part of the random variation in Cs-137 distribution is not a result of soil redistribution. Fortunately, the random variation can be overcome statistically by increasing independent sample numbers on both reference and measuring sites and by interpreting soil redistribution rates in terms of mean value for a uniform landform unit or contour transect (slope position) because the random errors tend to cancel out if averaged over a uniform area. Published by Elsevier B.V. C1 [Zhang, G. H.; Wei, X.] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Zhang, X. C. (John)] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Zhang, XC (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM john.Zhang@ars.usda.gov; ghzhang@bnu.edu.cn; weixin2007@bnu.edu.cn FU open project: Tracking soil redistribution on hillslopes - State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University [2011-KF-12] FX The work was partially funded by the open project: Tracking soil redistribution on hillslopes (project no. 2011-KF-12) funded by the State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University. NR 38 TC 5 Z9 5 U1 2 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD FEB PY 2015 VL 239 BP 186 EP 194 DI 10.1016/j.geoderma.2014.10.004 PG 9 WC Soil Science SC Agriculture GA AY7XA UT WOS:000347768000018 ER PT J AU Alonso, DG Oliveira, RS Hall, KE Koskinen, WC Constantin, J Mislankar, S AF Alonso, Diego G. Oliveira, Rubem S., Jr. Hall, Kathleen E. Koskinen, William C. Constantin, Jamil Mislankar, Suresh TI Changes in sorption of indaziflam and three transformation products in soil with aging SO GEODERMA LA English DT Article DE Bound residue; Metabolite; Degradation; Persistence ID ORGANIC-COMPOUNDS; TROPICAL SOILS; DESORPTION; RESIDUES; BIOAVAILABILITY; DEGRADATION; TIME; ACID; METABOLITES; PESTICIDES AB To evaluate environmental risks of pesticides in soil, it is necessary to determine aging effects on sorption processes. Few studies have been carried out on their metabolites. The effect of incubation time on sorption of indaziflam and indaziflam-triazinediamine (FDAT), indaziflam-triazine indanone (ITI) and indaziflamcarboxylic add (ICA) metabolites was determined on a mollisol and two depths of an oxisol. Soils were treated with [C-14]-indaziflam and [C-14]-metabolites, incubated for 112 days, then sequentially extracted with 0.01 N CaCl2, acetonitrile:water (4:1), and acetonitrile. Apparent sorption coefficients (K-d,K-app) were calculated based on compound concentrations in solution and sorbed to soil. Decreases in total remaining chemicals were due to mineralization and formation of bound residues. K-d,K-app values increased in the first two weeks of incubation, then tended to equilibrate. On average, sorption followed ITI> indaziflam >> ICA > FDAT. A significant increase in the sorption potentials of compounds and formation of bound residues was observed with the increase of incubation time (especially within the first 14 days), which would decrease the mobility potential of these molecules in the soil and therefore the possible contamination of underground water sources. (C) 2014 Elsevier B.V. All rights reserved. C1 [Alonso, Diego G.; Oliveira, Rubem S., Jr.; Constantin, Jamil] Univ Estadual Maringa, Ctr Adv Studies Weed Res NAPD, Dept Agron, BR-87020900 Maringa, Parana, Brazil. [Hall, Kathleen E.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Koskinen, William C.] USDA ARS, St Paul, MN 55108 USA. [Mislankar, Suresh] Bayer Crop Sci LP, Environm Fate & Exposure Assessment, RTP, Res Triangle Pk, NC 27709 USA. RP Oliveira, RS (reprint author), Univ Estadual Maringa, Ctr Adv Studies Weed Res NAPD, Dept Agron, Av Colombo 5790, BR-87020900 Maringa, Parana, Brazil. EM rsojunior@uem.br OI Oliveira, Rubem/0000-0002-5222-8010 FU CNPq (Brazil) [200308/2010-6] FX We thank the CNPq (Grant 200308/2010-6) (Brazil) for financial support for this research and Bayer Crop Science for supplying 14C-labeled molecules. NR 33 TC 5 Z9 5 U1 3 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD FEB PY 2015 VL 239 BP 250 EP 256 DI 10.1016/j.geoderma.2014.11.008 PG 7 WC Soil Science SC Agriculture GA AY7XA UT WOS:000347768000025 ER PT J AU Li, Y Chen, HL Bannick, N Henry, M Holm, AN Metwali, A Urban, JF Rothman, PB Weiner, GJ Blazar, BR Elliott, DE Ince, MN AF Li, Yue Chen, Hung-Lin Bannick, Nadine Henry, Michael Holm, Adrian N. Metwali, Ahmed Urban, Joseph F., Jr. Rothman, Paul B. Weiner, George J. Blazar, Bruce R. Elliott, David E. Ince, M. Nedim TI Intestinal Helminths Regulate Lethal Acute Graft-versus-Host Disease and Preserve the Graft-versus-Tumor Effect in Mice SO JOURNAL OF IMMUNOLOGY LA English DT Article ID BONE-MARROW-TRANSPLANTATION; HEMATOPOIETIC-CELL TRANSPLANTATION; INFLAMMATORY-BOWEL-DISEASE; CD25(+) T-CELLS; TGF-BETA; HELIGMOSOMOIDES-POLYGYRUS; FOXP3 EXPRESSION; CUTTING EDGE; NKT CELLS; SUPPRESSION AB Donor T lymphocyte transfer with hematopoietic stem cells suppresses residual tumor growth (graft-versus-tumor [GVT]) in cancer patients undergoing bone marrow transplantation (BMT). However, donor T cell reactivity to host organs causes severe and potentially lethal inflammation called graft-versus-host disease (GVHD). High-dose steroids or other immunosuppressive drugs are used to treat GVHD that have limited ability to control the inflammation while incurring long-term toxicity. Novel strategies are needed to modulate GVHD, preserve GVT, and improve the outcome of BMT. Regulatory T cells (Tregs) control alloantigen-sensitized inflammation of GVHD, sustain GVT, and prevent mortality in BMT. Helminths colonizing the alimentary tract dramatically increase the Treg activity, thereby modulating intestinal or systemic inflammatory responses. These observations led us to hypothesize that helminths can regulate GVHD and maintain GVT in mice. Acute GVHD was induced in helminth (Heligmosomoides polygyrus)-infected or uninfected BALB/c recipients of C57BL/6 donor grafts. Helminth infection suppressed donor T cell inflammatory cytokine generation and reduced GVHD-related mortality, but maintained GVT. H. polygyrus colonization promoted the survival of TGF-beta-generating recipient Tregs after a conditioning regimen with total body irradiation and led to a TGF-beta-dependent in vivo expansion/maturation of donor Tregs after BMT. Helminths did not control GVHD when T cells unresponsive to TGF-beta-mediated immune regulation were used as donor T lymphocytes. These results suggest that helminths suppress acute GVHD using Tregs and TGF-beta-dependent pathways in mice. Helminthic regulation of GVHD and GVT through intestinal immune conditioning may improve the outcome of BMT. C1 [Li, Yue; Chen, Hung-Lin; Holm, Adrian N.; Metwali, Ahmed; Rothman, Paul B.; Weiner, George J.; Elliott, David E.; Ince, M. Nedim] Univ Iowa, Dept Internal Med, Carver Coll Med, Iowa City, IA 52242 USA. [Bannick, Nadine; Henry, Michael] Univ Iowa, Dept Mol Physiol & Biophys, Carver Coll Med, Iowa City, IA 52242 USA. [Henry, Michael; Weiner, George J.; Ince, M. Nedim] Univ Iowa, Carver Coll Med, Holden Comprehens Canc Ctr, Iowa City, IA 52242 USA. [Urban, Joseph F., Jr.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Blazar, Bruce R.] Univ Minnesota, Div Blood & Marrow Transplantat, Dept Pediat, Minneapolis, MN 55455 USA. RP Ince, MN (reprint author), Univ Iowa Hosp & Clin, Dept Internal Med, Div Gastroenterol & Hepatol, 4546 John Colloton Pavil,200 Hawkins Dr, Iowa City, IA 52242 USA. EM m-nedim-ince@uiowa.edu OI Urban, Joseph/0000-0002-1590-8869 FU National Institute of Diabetes and Digestive and Kidney Diseases [K08 DK082913]; National Cancer Institute [P50 CA097274]; American Cancer Society [ACS-IRG-77-004-31]; National Institute of Allergy and Infectious Diseases [R01 AI34495]; National Heart, Lung, and Blood Institute [R01 HL56067, R01 HL11879]; VA Merit Award FX This work was supported by Grants K08 DK082913 from the National Institute of Diabetes and Digestive and Kidney Diseases, Lymphoma SPORE P50 CA097274 from the National Cancer Institute, ACS-IRG-77-004-31 from the American Cancer Society and administered through the Holden Comprehensive Cancer Center at the University of Iowa (to M.N.I.), R01 AI34495 from the National Institute of Allergy and Infectious Diseases, and R01 HL56067 and R01 HL11879 from the National Heart, Lung, and Blood Institute (to B. R. B.), as well as a VA Merit Award (to D. E. E.). NR 64 TC 5 Z9 5 U1 1 U2 4 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD FEB 1 PY 2015 VL 194 IS 3 BP 1011 EP 1020 DI 10.4049/jimmunol.1303099 PG 10 WC Immunology SC Immunology GA AZ3NK UT WOS:000348134000021 PM 25527786 ER PT J AU Ghanem, ENB Clark, S Du, XG Wu, DY Camilli, A Leong, JM Meydani, SN AF Ghanem, Elsa N. Bou Clark, Stacie Du, Xiaogang Wu, Dayong Camilli, Andrew Leong, John M. Meydani, Simin N. TI The alpha-Tocopherol Form of Vitamin E Reverses Age-Associated Susceptibility to Streptococcus pneumoniae Lung Infection by Modulating Pulmonary Neutrophil Recruitment SO JOURNAL OF IMMUNOLOGY LA English DT Article ID MURINE PNEUMOCOCCAL PNEUMONIA; E SUPPLEMENTATION; HEPOXILIN A(3); T-CELLS; TRANSEPITHELIAL MIGRATION; CYTOKINE RESPONSE; IMMUNE-RESPONSE; OLDER-ADULTS; MICE; INFLAMMATION AB Streptococcus pneumoniae infections are an important cause of morbidity and mortality in older patients. Uncontrolled neutrophil-driven pulmonary inflammation exacerbates this disease. To test whether the alpha-tocopherol (alpha-Toc) form of vitamin E, a regulator of immunity, can modulate neutrophil responses as a preventive strategy to mitigate the age-associated decline in resistance to S. pneumoniae, young (4 mo) and old (22-24 mo) C57BL/6 mice were fed a diet containing 30-PPM (control) or 500-PPM (supplemented) alpha-Toc for 4 wk and intratracheally infected with S. pneumoniae. Aged mice fed a control diet were exquisitely more susceptible to S. pneumoniae than young mice. At 2 d postinfection, aged mice suffered 1000-fold higher pulmonary bacterial burden, 2.2-fold higher levels of neutrophil recruitment to the lung, and a 2.25-fold higher rate of lethal septicemia. Strikingly, alpha-Toc supplementation of aged mice resulted in a 1000-fold lower bacterial lung burden and full control of infection. This alpha-Toc-induced resistance to pneumococcal challenge was associated with a 2-fold fewer pulmonary neutrophils, a level comparable to S. pneumoniae-challenged, conventionally fed young mice. alpha-Toc directly inhibited neutrophil egress across epithelial cell monolayers in vitro in response to pneumococci or hepoxilin-A(3), an eicosanoid required for pneumococcus-elicited neutrophil transepithelial migration. alpha-Toc altered expression of multiple epithelial and neutrophil adhesion molecules involved in migration, including CD55, CD47, CD18/CD11b, and ICAM-1. These findings suggest that alpha-Toc enhances resistance of aged mice to bacterial pneumonia by modulating the innate immune response, a finding that has potential clinical significance in combating infection in aged individuals through nutritional intervention. C1 [Ghanem, Elsa N. Bou; Clark, Stacie; Camilli, Andrew; Leong, John M.] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA. [Du, Xiaogang; Wu, Dayong; Meydani, Simin N.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Camilli, Andrew] Howard Hughes Med Inst, Boston, MA 02111 USA. RP Meydani, SN (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM john.leong@tufts.edu; simin.meydani@tufts.edu FU Tufts Collaborates [M230169]; U.S. Department of Agriculture [58-1950-0-014]; A.S.P.E.N. Rhoads Research Foundation FX This work was supported in part by Tufts Collaborates Grant M230169 (to A.C., J.M.L., and S.N.M.), U.S. Department of Agriculture Contract 58-1950-0-014 (to S.N.M.), and the A.S.P.E.N. Rhoads Research Foundation 2013 and 2014 Abbott Nutrition grant (to E.N.B.G.). NR 58 TC 3 Z9 3 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD FEB 1 PY 2015 VL 194 IS 3 BP 1090 EP 1099 DI 10.4049/jimmunol.1402401 PG 10 WC Immunology SC Immunology GA AZ3NK UT WOS:000348134000030 ER PT J AU Hunt, SL Kadavy, KC AF Hunt, Sherry L. Kadavy, Kem C. TI Discussion of "Aeration, Flow Instabilities, and Residual Energy on Pooled Stepped Spillways of Embankment Dams" by Stefan Felder and Hubert Chanson SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING LA English DT Editorial Material AB Congratulations to the authors for extending the knowledge in stepped spillways of embankment dams. This comprehensive study for comparing flat-stepped and pooled stepped spillways offers alternatives for design engineers and highlights some significant areas (i.e., aeration, inception point, flow properties as they relate to air concentrations and velocity, and energy dissipation) of interest for engineers tasked with designing these structures. Despite the design alternative and the increased energy dissipation provided by the pooled stepped spillway (i.e., theta = 26.6 degrees) that is more indicative of an embankment dam, the discussers are interested in the applicability of the pooled stepped spillways. Besides instabilities noted by the authors, the pooled stepped spillways also provide an element of complexity in the constructability of the stepped spillway and consequently, an added expense in the construction. The discussers are interested in what the desire application (i.e., increased energy dissipation and fish passage) of these structures are. In addition to use, the discussers wish to comment on some particular areas of interest regarding the experimental setup, the inception point data and the air-water flow properties. C1 [Hunt, Sherry L.; Kadavy, Kem C.] USDA ARS, Hydraul Engn Res Unit, Stillwater, OK 74075 USA. RP Hunt, SL (reprint author), USDA ARS, Hydraul Engn Res Unit, 1301 N Western Rd, Stillwater, OK 74075 USA. EM sherry.hunt@ars.usda.gov; kem.kadavy@ars.usda.gov NR 12 TC 0 Z9 0 U1 1 U2 3 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 EI 1943-4774 J9 J IRRIG DRAIN ENG JI J. Irrig. Drainage Eng-ASCE PD FEB PY 2015 VL 141 IS 2 AR 07014038 DI 10.1061/(ASCE)IR.1943-4774.0000627 PG 3 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA AZ3TN UT WOS:000348149600004 ER PT J AU Cai, ZJ Wang, BR Xu, MG Zhang, HM He, XH Zhang, L Gao, SD AF Cai, Zejiang Wang, Boren Xu, Minggang Zhang, Huimin He, Xinhua Zhang, Lu Gao, Suduan TI Intensified soil acidification from chemical N fertilization and prevention by manure in an 18-year field experiment in the red soil of southern China SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Acidification rate; Base cations; Exchangeable Al; Inhibition of soil acidification; Manure; Soil pH ID ACID SOILS; NITROGEN-FERTILIZER; ASH ALKALINITY; WINTER-WHEAT; INITIAL PH; RESIDUES; ALUMINUM; YIELD; NEUTRALIZATION; SOLUBILITY AB Soil acidification from chemical N fertilization has worsened and is a major yield-limiting factor in the red soil (Ferralic Cambisol) of southern China. Assessment of the acidification process under field conditions over a long term is essential to develop strategies for maintaining soil productivity. The objective of this study was to quantify soil acidification rates from chemical fertilizers and determine the amount of manure needed to inhibit the acidification process. A long-term experiment with various fertilizations was carried out during 1990-2008 in a wheat-corn cropping system in the red soil of southern China. Treatments included non-fertilized control, chemical N only (N), chemical N and P (NP), chemical N, P and K (NPK), pig manure only (M), and NPK plus M (NPKM; 70 % total N from M). All N treatments had an input of 300 kg N ha(-1) year(-1). Annual soil sampling was carried out for pH measurement and acidity analysis. Soil pH decreased sharply from an initial pH of 5.7 and then stabilized after 8 to 12 years of fertilization in the N, NP, and NPK treatments with a final pH of 4.2, 4.5, and 4.5, respectively. These three treatments significantly increased soil exchangeable acidity dominated by Al, decreased soil exchangeable base cations (Ca2+ and Mg2+), and elevated acidification rates (3.2-3.9 kmol H+ ha(-1) year(-1)). In contrast, the manure applications (M or NPKM) showed either an increase or no change in soil pH and increases in soil exchangeable base cations. Urea application to the intensive cropping system accelerated acidification of the red soil during the 18-year field experiment. As 70 % or more total N source, continuous manure application can fully prevent or reverse red soil acidification process. As an effective animal waste management tool, manure incorporation into the acidic soil can promote the overall agricultural sustainability. C1 [Cai, Zejiang; Wang, Boren; Xu, Minggang; Zhang, Huimin; Zhang, Lu] Qiyang Agro Ecosyst Natl Field Expt Stn, Qiyang 426182, Hunan, Peoples R China. [Cai, Zejiang; Wang, Boren; Xu, Minggang; Zhang, Huimin; Zhang, Lu] Chinese Acad Agr Sci, Minist Agr, Key Lab Crop Nutr & Fertilizat, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. [He, Xinhua] Univ Western Australia, Ctr Excellence Ecohydrol, Crawley, WA 6009, Australia. [He, Xinhua] Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia. [Gao, Suduan] ARS, San Joaquin Valley Agr Sci Ctr, USDA, Parlier, CA 93648 USA. RP Xu, MG (reprint author), Chinese Acad Agr Sci, Minist Agr, Key Lab Crop Nutr & Fertilizat, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. EM mgxu@caas.ac.cn RI He, Xinhua/B-8047-2015 OI He, Xinhua/0000-0002-5570-3454 FU National Science Foundation of China [41301309]; National Basic Research Program [2014CB441001]; National Science and Technology Development Program [2012BAD05B05]; National Nonprofit Institute Research Grant of CAAS [IARRP-2014-10] FX Financial support was obtained from the National Science Foundation of China (41301309), the National Basic Research Program (2014CB441001), the National Science and Technology Development Program (2012BAD05B05), and the National Nonprofit Institute Research Grant of CAAS (IARRP-2014-10). We are very grateful to Professor Daniel Murphy of the University of Western Australia for his constructional comments and suggestions. Mention of trademark, propriety product, or vendor in this article does not constitute a guarantee or warranty of the product by the USDA-ARS nor does it imply approval to the exclusion of other products or vendors that may be suitable. NR 56 TC 8 Z9 13 U1 9 U2 63 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD FEB PY 2015 VL 15 IS 2 BP 260 EP 270 DI 10.1007/s11368-014-0989-y PG 11 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA AZ3MN UT WOS:000348131800002 ER PT J AU Vahdati, K Pourtaklu, SM Karimi, R Barzehkar, R Amiri, R Mozaffari, M Woeste, K AF Vahdati, Kourosh Pourtaklu, Somayeh Mohseni Karimi, Rouhollah Barzehkar, Rouhollah Amiri, Reza Mozaffari, Mohammad Woeste, Keith TI Genetic diversity and gene flow of some Persian walnut populations in southeast of Iran revealed by SSR markers SO PLANT SYSTEMATICS AND EVOLUTION LA English DT Article DE Genetic structure; Gene flow; Juglans regia; Microsatellites; Polymorphism ID JUGLANS-REGIA L; FRAGMENT-LENGTH-POLYMORPHISMS; MOLECULAR CHARACTERIZATION; MICROSATELLITE MARKERS; L. GENOTYPES; DNA; IDENTIFICATION; JUGLANDACEAE; CULTIVARS; FREQUENCY AB Iran is reported to be a center of diversity for Juglans regia and wild walnut trees are found in virtually every corner of the country. Thus Iran is considered a rich natural pool of walnut germplasm for developing improved genotypes. Kerman province is the most important Iranian province for walnut culture and has the largest walnut plantations in Iran. Genetic structure and gene flow were analyzed in six walnut populations of this province using 17 microsatellite loci. The number of alleles per locus ranged from 4 to 11, with a total of 147 alleles and 5.16 effective alleles per locus. The polymorphism information content for the loci ranged from 0.56 to 0.82. The expected heterozygosity (H (e)) for the populations ranged from 0.65 to 0.87. There were differences between populations regarding the number of effective alleles and Shannon's information index (I). In all populations, observed heterozygosity (H (o)) was lower than expected, but diversity within the populations was high (I = 1.5) and many of the private alleles were present at relatively high frequency. The average F (st) value was 0.08. The level of gene flow based on F (st) was high (N (m) = 3.01), which meant that the high level of genetic diversity maintained within each population was less susceptible to genetic drift. The geographical proximity of the populations was not correlated with their level of genetic relatedness. These results imply the high potential of walnut populations of Kerman province for breeding programs. C1 [Vahdati, Kourosh; Pourtaklu, Somayeh Mohseni] Univ Tehran, Coll Aburaihan, Dept Hort, Tehran, Iran. [Karimi, Rouhollah] Malayer Univ, Dept Hort & Landscape Engn, Fac Agr, Malayer, Iran. [Barzehkar, Rouhollah] Agr Bank Iran, Cent Branch Bank Keshavarzi, Syrjan, Iran. [Amiri, Reza] Univ Tehran, Dept Agron & Crop Breeding Sci, Coll Aburaihan, Tehran, Iran. [Mozaffari, Mohammad] Agr & Nat Resources Res Ctr, Kerman, Iran. [Woeste, Keith] Purdue Univ, Dept Forestry & Nat Resources, Hardwood Tree Improvement & Regenerat Ctr, USDA Forest Serv, W Lafayette, IN 47907 USA. RP Vahdati, K (reprint author), Univ Tehran, Coll Aburaihan, Dept Hort, Tehran, Iran. EM kvahdati@ut.ac.ir; s_mohsenipour@yahoo.com; karimy2006@yahoo.com; rbarzehkar@yahoo.com; ramiri@ut.ac.ir; m_mozaffari2003@yahoo.com; woeste@purdue.edu FU University of Tehran; Iran National Science Foundation (INSF) FX The authors thank the University of Tehran and the Iran National Science Foundation (INSF) for their support of this research. The authors also acknowledge Dr. Thierry Marcel (Wageningen UR Plant Breeding, Wageningen, The Netherlands), Dr. Pedro Martinez Gomez (Department of Plant Breeding, CEBAS-CSIC, Murcia, Spain) and Dr. Mohsen Ebrahimi (Department of Agronomy and Crop Breeding Sciences, College of Aburaihan, University of Tehran, Tehran, Iran) for pre-reviewing the manuscript. NR 41 TC 2 Z9 2 U1 6 U2 26 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0378-2697 EI 1615-6110 J9 PLANT SYST EVOL JI Plant Syst. Evol. PD FEB PY 2015 VL 301 IS 2 BP 691 EP 699 DI 10.1007/s00606-014-1107-8 PG 9 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA AZ3ID UT WOS:000348119800017 ER PT J AU Evans, EW Jacques, PF Dallal, GE Sacheck, J Must, A AF Evans, E. Whitney Jacques, Paul F. Dallal, Gerard E. Sacheck, Jennifer Must, Aviva TI The role of eating frequency on total energy intake and diet quality in a low-income, racially diverse sample of schoolchildren SO PUBLIC HEALTH NUTRITION LA English DT Article DE Childhood obesity; Meal patterns; Snack patterns; Energy intake; Diet quality ID BODY-MASS INDEX; SCHOOL-AGED CHILDREN; US CHILDREN; CHILDHOOD OBESITY; SNACKING PATTERNS; UNITED-STATES; FOOD RECORDS; PROJECT EAT; ADOLESCENTS; TRENDS AB Objective The relationship of meal and snacking patterns with overall dietary intake and relative weight in children is unclear. The current study was done to examine how eating, snack and meal frequencies relate to total energy intake and diet quality. Design The cross-sectional associations of eating, meal and snack frequencies with total energy intake and diet quality, measured by the Healthy Eating Index 2005 (HEI-2005), were examined in separate multivariable mixed models. Differences were examined between elementary school-age participants (9-11 years) and adolescents (12-15 years). Setting Two non-consecutive 24 h diet recalls were collected from children attending four schools in the greater Boston area, MA, USA. Subjects One hundred and seventy-six schoolchildren, aged 9-15 years. Results Overall, 82 % of participants consumed three daily meals. Eating, meal and snack frequencies were statistically significantly and positively associated with total energy intake. Each additional reported meal and snack was associated with an 185 % and a 94 % increase in total energy intake, respectively (P<0001). The relationships of eating, meal and snack frequencies with diet quality differed by age category. In elementary school-age participants, total eating occasions and snacks increased HEI-2005 score. In adolescents, each additional meal increased HEI-2005 score by 540 points (P=001), whereas each additional snack decreased HEI-2005 score by 273 points (P=0006). Conclusions Findings suggest that snacking increases energy intake in schoolchildren. Snacking is associated with better diet quality in elementary school-age children and lower diet quality in adolescents. Further research is needed to elucidate the role of snacking in excess weight gain in children and adolescents. C1 [Evans, E. Whitney] Brown Univ, Sch Med, Weight Control & Diabet Res Ctr, Providence, RI 02903 USA. [Jacques, Paul F.; Dallal, Gerard E.; Sacheck, Jennifer; Must, Aviva] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Jacques, Paul F.; Dallal, Gerard E.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Must, Aviva] Tufts Univ, Sch Med, Boston, MA 02111 USA. RP Evans, EW (reprint author), Brown Univ, Sch Med, Weight Control & Diabet Res Ctr, 196 Richmond St, Providence, RI 02903 USA. EM whitney_evans@brown.edu FU National Institutes of Health (NIH) [NIH R01HL106160, NIDDK46200]; National Research Service Award FX Financial support: This study was funded, in part, by the National Institutes of Health (NIH) (grant number NIH R01HL106160), (grant number NIDDK46200 to Boston Nutrition Obesity Research Center); and a National Research Service Award for the NIDDK T32 Research Training Program in Nutrition and Chronic Disease. The NIH had no role in the design, analysis or writing of this article. NR 46 TC 7 Z9 7 U1 1 U2 5 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD FEB PY 2015 VL 18 IS 3 BP 474 EP 481 DI 10.1017/S1368980014000470 PG 8 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA AZ4JC UT WOS:000348186300011 PM 24780506 ER PT J AU Jahns, L McDonald, L Wadsworth, A Morin, C Liu, Y Nicklas, T AF Jahns, Lisa McDonald, Leander Wadsworth, Ann Morin, Charles Liu, Yan Nicklas, Theresa TI Barriers and facilitators to following the Dietary Guidelines for Americans reported by rural, Northern Plains American-Indian children SO PUBLIC HEALTH NUTRITION LA English DT Article DE American-Indian children; Nominal group technique; Dietary Guidelines for Americans; Barriers and facilitators ID NOMINAL GROUP TECHNIQUE; LIFE-STYLE INTERVENTION; CARDIOVASCULAR-DISEASE; PHYSICAL-ACTIVITY; NATIVE-AMERICAN; HEALTH-STATUS; US CHILDREN; OBESITY; OVERWEIGHT; PREVENTION AB Objective The Dietary Guidelines for Americans (DGA) promote healthy dietary choices for all Americans aged 2 years and older; however, the majority of Americans do not meet recommendations. The goal of the present study was to identify both barriers and facilitators to adherence to DGA recommendations for consumption of five recommended food groups: grains (specifically whole grains), vegetables, fruits, meat/beans and milk (specifically reduced-fat/non-fat), among American-Indian children. Design Nominal group technique sessions were conducted to identify and prioritize children's perceived barriers and facilitators to following the DGA, as presented in the MyPyramid' consumer education icon. After response generation to a single question about each food group (e.g. What sorts of things make it harder (or easier) for kids to follow the MyPyramid recommendation for vegetables?'), children individually ranked their top five most salient responses. Ranked responses are presented verbatim. Setting A rural Northern Plains American-Indian reservation, USA. Subjects Sixty-one self-selected fifth-grade children. Results Core barriers for all food groups studied included personal preference (i.e. don't like') and environmental (i.e. cost too much'; store is too far to get them'; grandma don't have'). Core facilitators included suggestions, i.e. make a garden and plant vegetables'; tell your friends to eat healthy'. Conclusions Barriers and facilitators are dissimilar for individual food groups, suggesting that dietary interventions should target reduction of barriers and promotion of facilitators specific to individual food groups recommended by the DGA. C1 [Jahns, Lisa] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58203 USA. [McDonald, Leander; Wadsworth, Ann] Cankdeska Cikana Community Coll, Ft Totten, ND USA. [Morin, Charles] Tate Topa Tribal Sch, Ft Totten, ND USA. [Liu, Yan; Nicklas, Theresa] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Jahns, L (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. EM lisa.jahns@ars.usda.gov OI Jahns, Lisa/0000-0002-1828-6962 FU US Department of Agriculture (USDA), Agricultural Research Service [USDA 5450-51000-049-00D] FX Financial support: This work was supported the US Department of Agriculture (USDA), Agricultural Research Service (grant number USDA 5450-51000-049-00D). The contents of this publication do not necessarily reflect the views or policies of the USDA or the Agricultural Research Service, nor does mention of trade names, commercial products or organizations imply endorsement from the US government. The USDA had no role in the design, analysis or writing of this article. NR 51 TC 0 Z9 0 U1 4 U2 13 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD FEB PY 2015 VL 18 IS 3 BP 482 EP 489 DI 10.1017/S136898001400041X PG 8 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA AZ4JC UT WOS:000348186300012 PM 24679830 ER PT J AU Whittaker, G Barnhart, BL Srinivasan, R Arnold, JG AF Whittaker, Gerald Barnhart, Bradley L. Srinivasan, Raghavan Arnold, Jeffrey G. TI Cost of areal reduction of gulf hypoxia through agricultural practice SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Hypoxia; Eutrophication; Gulf of Mexico; Nonpoint source pollution; Agricultural incentive policy ID CONSERVATION RESERVE PROGRAM; OF-MEXICO; WATER-QUALITY; IMPACTS; VOLUME; MODEL AB A major share of the area of hypoxic growth in the Northern Gulf of Mexico has been attributed to nutrient runoff from agricultural fields, but no estimate is available for the cost of reducing Gulf hypoxic area using agricultural conservation practices. We apply the Soil and Water Assessment Tool using observed daily weather to simulate the reduction in nitrogen loading in the Upper Mississippi River Basin (UMRB) that would result from enrolling all row crop acreage in the Conservation Reserve Program (CRP). Nitrogen loadings at the outlet of the UMRB are used to predict Gulf hypoxic area, and net cash farm rent is used as the price for participation in the CRP. Over the course of the 42 year simulation, direct CRP costs total more than $388 billion, and the Inter-Governmental Task Force goal of hypoxic area less than 5000 square kilometers is met in only two years. Published by Elsevier B.V. C1 [Whittaker, Gerald; Barnhart, Bradley L.] ARS, USDA, Corvallis, OR 97331 USA. [Srinivasan, Raghavan] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [Srinivasan, Raghavan] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Arnold, Jeffrey G.] ARS, USDA, Temple, TX USA. RP Whittaker, G (reprint author), ARS, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM Jerry.Whittaker@ars.usda.gov RI Srinivasan, R/D-3937-2009 NR 27 TC 1 Z9 1 U1 3 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 1 PY 2015 VL 505 BP 149 EP 153 DI 10.1016/j.scitotenv.2014.09.101 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY6CJ UT WOS:000347654900014 PM 25461017 ER PT J AU Fairbairn, DJ Karpuzcu, ME Arnold, WA Barber, BL Kaufenberg, EF Koskinen, WC Novak, PJ Rice, PJ Swackhamer, DL AF Fairbairn, David J. Karpuzcu, M. Ekrem Arnold, William A. Barber, Brian L. Kaufenberg, Elizabeth F. Koskinen, William C. Novak, Paige J. Rice, Pamela J. Swackhamer, Deborah L. TI Sediment-water distribution of contaminants of emerging concern in a mixed use watershed SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Sediment; Surface water; CEC; Agriculture; PPCP; Urban ID ORGANIC POLLUTANT SORPTION; PERSONAL CARE PRODUCTS; NATURAL SEDIMENTS; PHARMACEUTICALS; SOIL; ATRAZINE; MATTER; FATE; ANTIBIOTICS; PHOTOLYSIS AB This study evaluated the occurrence and distribution of 15 contaminants of emerging concern (CECs) in stream water and sediments in the Zumbro River watershed in Minnesota and compared these with sub-watershed land uses. Sixty pairs of sediment and water samples were collected across all seasons from four stream sites for over two years and analyzed for selected personal care products, pesticides, human and veterinary medications, and phytoestrogens. Spatial and temporal analyses indicate that pharmaceuticals and personal care products (urban/residential CECs) are significantly elevated in water and/or sediment at sites with greater population density (>100 people/km(2)) and percentage of developed land use (>8% of subwatershed area) than those with less population density and land area under development Significant spatial variations of agricultural pesticides in water and sediment were detectable, even though all sites had a high percentage of agricultural land use. Seasonality in CEC concentration was observed in water but not in sediment, although sediment concentrations of three CECs did vary between years. Average measured non-equilibrium distribution coefficients exceeded equilibrium hydrophobic partitioning-based predictions for 5 of the 7 detected CECs by at least an order of magnitude. Agreement of measured and predicted distribution coefficients improved with increasing hydrophobicity and in-stream persistence. The more polar and degradable CECs showed greater variability in measured distributions across different sampling events. Our results confirm that CECs are present in urban and agricultural stream sediments, including those CECs that would typically be thought of as non-sorptive based on their log K-ow values. These results and the observed patterns of sediment and water distributions augment existing information to improve prediction of CEC fate and transport, leading to more accurate assessments of exposure and risk to surface water ecosystems. (C) 2014 Elsevier B.V. All rights reserved. C1 [Fairbairn, David J.; Karpuzcu, M. Ekrem; Kaufenberg, Elizabeth F.; Swackhamer, Deborah L.] Univ Minnesota, Water Resources Ctr, St Paul, MN 55108 USA. [Arnold, William A.; Novak, Paige J.] Univ Minnesota, Minneapolis, MN 55455 USA. [Barber, Brian L.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Koskinen, William C.; Rice, Pamela J.] Univ Minnesota, ARS, USDA, St Paul, MN 55108 USA. RP Fairbairn, DJ (reprint author), 520 Lafayette Rd, St Paul, MN 55101 USA. EM david.fairbairn@state.mn.us RI Karpuzcu, Mahmut Ekrem/L-8420-2014; OI Karpuzcu, Mahmut Ekrem/0000-0002-6245-9621; Arnold, William/0000-0003-0814-5469 FU Minnesota Environment and Natural Resources Trust Fund [012-A2] FX This work was supported by the Minnesota Environment and Natural Resources Trust Fund (012-A2) as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR). We thank McGhie Betts, Inc (Rochester, MN) for the technical support on watershed characterization; Dr. Megan Kelly (Water Resources Science, University of Minnesota) for her assistance with sampling and sample analysis; and Dr. Gary Oehlert (Department of Statistics, University of Minnesota) for his advice on statistical design and analysis. NR 60 TC 9 Z9 10 U1 14 U2 128 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD FEB 1 PY 2015 VL 505 BP 896 EP 904 DI 10.1016/j.scitotenv.2014.10.046 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY6CJ UT WOS:000347654900090 PM 25461092 ER PT J AU McDonough, OT Lang, MW Hosen, JD Palmer, MA AF McDonough, Owen T. Lang, Megan W. Hosen, Jacob D. Palmer, Margaret A. TI Surface Hydrologic Connectivity Between Delmarva Bay Wetlands and Nearby Streams Along a Gradient of Agricultural Alteration SO WETLANDS LA English DT Article DE Geographically isolated wetlands; Non-perennial streams; Delmarva bays; Wetland restoration; Surface hydrologic connectivity ID GEOGRAPHICALLY ISOLATED WETLANDS; SOUTHEASTERN UNITED-STATES; ATLANTIC COASTAL-PLAIN; DEPRESSIONAL WETLANDS; USA; GROUNDWATER; SCIENCE; REGION; VALLEY AB Although recent U.S. Supreme Court rulings indicate surface hydrologic connectivity (SHC) between geographically isolated wetlands and nearby streams may be used, in part, to determine wetland jurisdictional status, and ecologic implications are considerable regardless of policies, wetland-stream SHC has rarely been quantified. Furthermore, the impact of cultivation and restoration on wetland-stream SHC is largely unknown. To help fill these knowledge gaps, we recorded SHC patterns during water year 2010 in non-perennial streams connecting Delmarva bay wetlands, which are commonly considered geographically isolated, and nearby perennial streams. We also evaluated how hydrologic wetland restoration impacts SHC relative to historical wetlands and native forested wetlands. Cumulative connection duration, number of connectivity transitions, mean connection duration, and maximum individual connection duration (D (max-c) ) were quantified. Forested wetlands were connected to perennial streams for a greater cumulative duration but exhibited fewer connectivity transitions relative to both historical and restored wetlands. SHC between historical and restored wetlands and nearby perennial streams did not differ with respect to any of the calculated metrics. Forested wetland-stream SHC was seasonally intermittent, exhibiting stream outflow from mid-fall to late-spring during periods of low evapotranspiration and elevated groundwater levels but lacking connectivity during summer months when evapotranspiration and groundwater were at an annual high and low, respectively. Historical and restored wetland-stream SHC was largely ephemeral, occurring in response to antecedent rainfall, particularly during winter and spring. Stepwise regression models describe cumulative connection duration and D (max-c) as a function of wetland, watershed, and non-perennial stream metrics including watershed relief, non-perennial stream slope, non-perennial stream length, and soil saturated hydraulic conductivity. Wetland-stream SHC has potential ecological implications, including provision of dispersal corridors for biota, biogeochemical processing of nutrients, and downstream delivery of energy, matter, and organisms, and is currently tied to wetland regulatory status in the U.S. C1 [McDonough, Owen T.; Hosen, Jacob D.; Palmer, Margaret A.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [McDonough, Owen T.; Hosen, Jacob D.; Palmer, Margaret A.] Univ Maryland, Chesapeake Biol Lab, Ctr Environm Sci, Solomons, MD 20688 USA. [Lang, Megan W.] US Forest Serv, USDA, Beltsville, MD 20705 USA. [Palmer, Margaret A.] Natl Socioenvironm Synth Ctr, Annapolis, MD 21401 USA. RP McDonough, OT (reprint author), Univ Maryland, Chesapeake Biol Lab, Ctr Environm Sci, Solomons, MD 20688 USA. EM otmcdonough@gmail.com RI Hosen, Jacob/F-9224-2013 OI Hosen, Jacob/0000-0003-2559-0687 FU Wetland Component of the National United States Department of Agriculture (USDA) Conservation Effects Assessment Project (CEAP); University of Maryland College Park Behavior-Ecology-Evolution-Systematics (BEES) graduate program FX Funding for this research came in part from the Wetland Component of the National United States Department of Agriculture (USDA) Conservation Effects Assessment Project (CEAP) and the University of Maryland College Park Behavior-Ecology-Evolution-Systematics (BEES) graduate program. We thank Danny Welch, Greg McCarty, Tom Fisher, Brian Laub, Tony Oesterling, Kristen Politano, Heather Chen, and Shelley Devereaux for their assistance in the field and lab. We are particularly grateful for the insightful comments provided by two anonymous reviewers. NR 50 TC 1 Z9 1 U1 2 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD FEB PY 2015 VL 35 IS 1 BP 41 EP 53 DI 10.1007/s13157-014-0591-5 PG 13 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AZ5BK UT WOS:000348235100005 ER PT J AU Berry, ED Wells, JE Bono, JL Woodbury, BL Kalchayanand, N Norman, KN Suslow, TV Lopez-Velasco, G Millnerc, PD AF Berry, Elaine D. Wells, James E. Bono, James L. Woodbury, Bryan L. Kalchayanand, Norasak Norman, Keri N. Suslow, Trevor V. Lopez-Velasco, Gabriela Millnerc, Patricia D. TI Effect of Proximity to a Cattle Feedlot on Escherichia coli O157:H7 Contamination of Leafy Greens and Evaluation of the Potential for Airborne Transmission SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID MULTIPLEX PCR ASSAYS; FRESH PRODUCE; SPINACH LEAVES; UNITED-STATES; O157-H7; SURVIVAL; SALMONELLA; LETTUCE; MANURE; SOIL AB The impact of proximity to a beef cattle feedlot on Escherichia coli O157:H7 contamination of leafy greens was examined. In each of 2 years, leafy greens were planted in nine plots located 60, 120, and 180 m from a cattle feedlot (3 plots at each distance). Leafy greens (270) and feedlot manure samples (100) were collected six different times from June to September in each year. Both E. coli O157:H7 and total E. coli bacteria were recovered from leafy greens at all plot distances. E. coli O157:H7 was recovered from 3.5% of leafy green samples per plot at 60 m, which was higher (P < 0.05) than the 1.8% of positive samples per plot at 180 m, indicating a decrease in contamination as distance from the feedlot was increased. Although E. coli O157:H7 was not recovered from air samples at any distance, total E. coli was recovered from air samples at the feedlot edge and all plot distances, indicating that airborne transport of the pathogen can occur. Results suggest that risk for airborne transport of E. coli O157:H7 from cattle production is increased when cattle pen surfaces are very dry and when this situation is combined with cattle management or cattle behaviors that generate airborne dust. Current leafy green field distance guidelines of 120 m (400 feet) may not be adequate to limit the transmission of E. coli O157: H7 to produce crops planted near concentrated animal feeding operations. Additional research is needed to determine safe set-back distances between cattle feedlots and crop production that will reduce fresh produce contamination. C1 [Berry, Elaine D.; Wells, James E.; Bono, James L.; Woodbury, Bryan L.; Kalchayanand, Norasak; Norman, Keri N.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Suslow, Trevor V.; Lopez-Velasco, Gabriela] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Millnerc, Patricia D.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD USA. RP Berry, ED (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Elaine.Berry@ars.usda.gov OI LOPEZ-VELASCO, GABRIELA/0000-0002-7840-3091 FU Center for Produce Safety, UC Davis; California Department of Food and Agriculture's Specialty Crop Block Grant Program [SCB10057] FX Support for this project was provided in part by the Center for Produce Safety, UC Davis, and the California Department of Food and Agriculture's Specialty Crop Block Grant Program (SCB10057). NR 56 TC 5 Z9 5 U1 0 U2 27 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD FEB PY 2015 VL 81 IS 3 BP 1101 EP 1110 DI 10.1128/AEM.02998-14 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AZ0CU UT WOS:000347914800032 PM 25452286 ER PT J AU Benfield, JA Rainbolt, GN Bell, PA Donovan, GH AF Benfield, Jacob A. Rainbolt, Gretchen Nurse Bell, Paul A. Donovan, Geoffrey H. TI Classrooms With Nature Views: Evidence of Differing Student Perceptions and Behaviors SO ENVIRONMENT AND BEHAVIOR LA English DT Article DE education; biophilia; restoration; nature; design ID HIGH-SCHOOL; HEALTH; WINDOW; CHILDREN; STRESS; ENVIRONMENTS; PREFERENCE; SURGERY AB Viewing peaceful natural environments has been shown to restore cognitive abilities and reduce physiological arousal. As such, visual access to the natural environment is becoming more commonplace in built environments. One exception to that trend is in educational settings where windowless classrooms are used to reduce outside distractions. The current study examines differences across multiple sections of a college writing course in two types of identically designed classroomsthose with a view of a natural setting and those with a view of a concrete retaining wall. Results showed that students in the natural view classrooms were generally more positive when rating the course. Students in the natural view condition also had higher end of semester grades, but no differences in attendance were observed between conditions. Such findings suggest that classrooms with natural views offer advantages and also suggest that the inclusion of natural elements in courses could facilitate positive perceptions and better grades. C1 [Benfield, Jacob A.] Penn State Univ Abington, Abington, PA 19001 USA. [Bell, Paul A.] Colorado State Univ, Ft Collins, CO 80523 USA. [Donovan, Geoffrey H.] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR USA. RP Benfield, JA (reprint author), Penn State Univ Abington, Div Social Sci, 1600 Woodland Rd, Abington, PA 19001 USA. EM jab908@psu.edu NR 33 TC 4 Z9 5 U1 6 U2 29 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0013-9165 EI 1552-390X J9 ENVIRON BEHAV JI Environ. Behav. PD FEB PY 2015 VL 47 IS 2 BP 140 EP 157 DI 10.1177/0013916513499583 PG 18 WC Environmental Studies; Psychology, Multidisciplinary SC Environmental Sciences & Ecology; Psychology GA AY7BC UT WOS:000347715200002 ER PT J AU Zeng, LH Pettigrew, WT AF Zeng, Linghe Pettigrew, William T. TI Combining ability, heritability, and genotypic correlations for lint yield and fiber quality of Upland cotton in delayed planting SO FIELD CROPS RESEARCH LA English DT Article DE General combining ability; Specific combining ability; Genotypic correlations; Plating date; Delayed planting ID DATE; TILLAGE; REGISTRATION; GERMPLASM; CULTIVAR; SYSTEMS; GROWTH; WHEAT AB Delayed planting is a necessary practice in Upland cotton (Gossypium hirsutum L) production when double cropping cotton with small grain crops. All current cultivars used for delayed planting were selected under environments of normal planting. The information about genetic values of parents in delayed planting will help determine if it is necessary for testing parents under environments of delayed planting before their use in development of cultivars for the delayed planting system. General combining ability (GCA), specific combining ability (SCA), broad sense heritability, and genetic correlation were compared between two treatments of planting date, the normal planting (mid-April to early May) and delayed planting (late May). Ten F-2 hybrid populations derived from a half diallel crossing design and their five parents were planted in 2012 and 2013 with four replicates each year. GCA effects for lint yield and two yield components of boll no. and seeds per boll were different between delayed planting and normal planting. Both direction changes of SCA effects for yield components were observed between treatments of planting date. However, effects of SCA x year were not significant in delayed planting. Reductions in broad sense heritability of yield traits were observed in the delayed planting (0.01-0.25) compared with that of the normal planting (0.10-0.88). Combining ability effects and heritability for fiber properties were similar between treatments of planting date. Genotypic correlation between lint yield and fiber strength became more negative, -0.83, in delayed planting compared with -0.34 in normal planting. The change of genotypic correlations between lint yield and fiber quality in the delayed planting implies an increase of complexity for interrelationships among fiber traits in the double cropped cotton. Overall, the results should inform cotton breeders the need of testing parents for lint yield under environments of delayed planting. Published by Elsevier B.V. C1 [Zeng, Linghe] USDA ARS, Crop Genet Res Unit, Delta Res Ctr, Stoneville, MS 38776 USA. [Pettigrew, William T.] USDA ARS, Crop Prod Res Unit, Delta Res Ctr, Stoneville, MS 38776 USA. RP Zeng, LH (reprint author), USDA ARS, Crop Genet Res Unit, Delta Res Ctr, Stoneville, MS 38776 USA. EM linghe.zeng@ars.usda.gov FU USDA-ARS [6402-21000-051-00D] FX This research was funded by USDA-ARS, Project No. 6402-21000-051-00D. NR 22 TC 1 Z9 2 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD FEB 1 PY 2015 VL 171 BP 176 EP 183 DI 10.1016/j.fcr.2014.10.004 PG 8 WC Agronomy SC Agriculture GA AY9JC UT WOS:000347865300018 ER PT J AU Thomson, JL Landry, AS Zoellner, JM Connell, C Madson, MB Molaison, EF Yadrick, K AF Thomson, Jessica L. Landry, Alicia S. Zoellner, Jamie M. Connell, Carol Madson, Michael B. Molaison, Elaine Fontenot Yadrick, Kathy TI Participant Adherence Indicators Predict Changes in Blood Pressure, Anthropometric Measures, and Self-Reported Physical Activity in a Lifestyle Intervention: HUB City Steps SO HEALTH EDUCATION & BEHAVIOR LA English DT Article DE African American; community health promotion; diet; health behavior; measurement issues; physical activity ID DOSE INFLUENCE OUTCOMES; TRIAL; HYPERTENSION; PROGRAM; IMPLEMENTATION; COMMUNITY; FIDELITY AB The objective of this secondary analysis was to evaluate the utility of several participant adherence indicators for predicting changes in clinical, anthropometric, dietary, fitness, and physical activity (PA) outcomes in a lifestyle intervention, HUB City Steps, conducted in a southern, African American cohort in 2010. HUB City Steps was a 6-month, community-engaged, multicomponent, noncontrolled intervention targeting hypertension risk factors. Descriptive indicators were constructed using two participant adherence measures, education session attendance (ESA) and weekly steps/day pedometer diary submission (PDS), separately and in combination. Analyses, based on data from 269 primarily African American adult participants, included bivariate tests of association and multivariable linear regression to determine significant relationships between seven adherence indicators and health outcome changes, including clinical, anthropometric, dietary, fitness, and PA measures. ESA indicators were significantly correlated with four health outcomes: body mass index (BMI), fat mass, low-density lipoprotein (LDL), and PA (-.29 r .23, p < .05). PDS indicators were significantly correlated with PA (r = .27, p < .001). Combination ESA/PDS indicators were significantly correlated with five health outcomes: BMI, percentage body fat (%BF), fat mass, LDL, and PA (r = -.26 to .29, p < .05). Results from the multivariate models indicated that the combination ESA/PDS indicators were the most significant predictors of changes for five outcomes%BF, fat mass, LDL diastolic blood pressure (DBP), and PAwhile ESA performed best for BMI only. For DBP, a one-unit increase in the continuous-categorical ESA/PDS indicator resulted in 0.3 mm Hg decrease. Implications for assessing participant adherence in community-based, multicomponent lifestyle intervention research are discussed. C1 [Thomson, Jessica L.] USDA ARS, Stoneville, MS 38776 USA. [Landry, Alicia S.; Connell, Carol; Madson, Michael B.; Molaison, Elaine Fontenot; Yadrick, Kathy] Univ So Mississippi, Hattiesburg, MS 39406 USA. [Zoellner, Jamie M.] Virginia Tech, Blacksburg, VA USA. RP Thomson, JL (reprint author), USDA ARS, Delta Human Nutr Res Program, 141 Expt Stn Rd,POB 225, Stoneville, MS 38776 USA. EM jessica.thomson@ars.usda.gov FU NIMHD NIH HHS [R24MD002787, R24 MD002787] NR 22 TC 1 Z9 1 U1 1 U2 5 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1090-1981 EI 1552-6127 J9 HEALTH EDUC BEHAV JI Health Educ. Behav. PD FEB PY 2015 VL 42 IS 1 BP 84 EP 91 DI 10.1177/1090198114537069 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AZ1DZ UT WOS:000347981700010 PM 24986913 ER PT J AU Mills, MK Nayduch, D Michel, K AF Mills, M. K. Nayduch, D. Michel, K. TI Inducing RNA interference in the arbovirus vector, Culicoides sonorensis SO INSECT MOLECULAR BIOLOGY LA English DT Article DE biting midge; vector-borne disease; orbivirus; reverse genetics ID BLUETONGUE-VIRUS; BITING MIDGES; AEDES-AEGYPTI; APOPTOSIS; VARIIPENNIS; INHIBITOR; CERATOPOGONIDAE; TRANSMISSION; REPLICATION; INFECTION AB Biting midges in the genus Culicoides are important vectors of arboviral diseases, including epizootic haemorrhagic disease, bluetongue and most likely Schmallenberg, which cause significant economic burdens worldwide. Research on these vectors has been hindered by the lack of a sequenced genome, the difficulty of consistent culturing of certain species and the absence of molecular techniques such as RNA interference (RNAi). Here, we report the establishment of RNAi as a research tool for the adult midge, Culicoides sonorensis. Based on previous research and transcriptome analysis, which revealed putative small interfering RNA pathway member orthologues, we hypothesized that adult C.sonorensis midges have the molecular machinery needed to perform RNA silencing. Injection of control double-stranded RNA targeting green fluorescent protein (dsGFP), into the haemocoel of 2-3-day-old adult female midges resulted in survival curves that support virus transmission. dsRNA injection targeting the newly identified C.sonorensis inhibitor of apoptosis protein 1 (CsIAP1) orthologue resulted in a 40% decrease of transcript levels and 73% shorter median survivals as compared with dsGFP-injected controls. These results reveal the conserved function of IAP1. Importantly, they also demonstrate the feasibility of RNAi by dsRNA injection in adult midges, which will greatly facilitate studies of the underlying mechanisms of vector competence in C.sonorensis. C1 [Mills, M. K.; Michel, K.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Nayduch, D.] USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Michel, K (reprint author), Kansas State Univ, Div Biol, Ackert Hall, Manhattan, KS 66506 USA. EM kmichel@ksu.edu FU National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R01AI095842]; USDA Agricultural Research Service [58-5430-1360] FX The authors thank Dr Lee Cohnsteadt, Mr James A. Kempert and Mr William E. Yarnell at the Arthropod-Borne Animal Disease Research Unit, USDA-ARS, Manhattan, KS, for provision of all adult C. sonorensis midges and housing materials used in this study. We further thank Drs Rollie Clem and W. Bart Bryant, Division of Biology, Kansas State University, for helpful discussions on IAP1. This is contribution no. 15-093-J from the Kansas Agricultural Experiment Station. Research reported in this publication was supported by The National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R01AI095842 (K.M.), and by Specific Cooperative Agreement 58-5430-1360 to K.M. from the USDA Agricultural Research Service. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies. NR 50 TC 5 Z9 5 U1 3 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD FEB PY 2015 VL 24 IS 1 BP 105 EP 114 DI 10.1111/imb.12139 PG 10 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA AY3YT UT WOS:000347516100009 PM 25293805 ER PT J AU Su, HH Tian, JC Naranjo, SE Romeis, J Hellmich, RL Shelton, AM AF Su, H. -H. Tian, J. -C. Naranjo, S. E. Romeis, J. Hellmich, R. L. Shelton, A. M. TI Bacillus thuringiensis plants expressing Cry1Ac, Cry2Ab and Cry1F are not toxic to the assassin bug, Zelus renardii SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Spodoptera frugiperda; Trichoplusia ni; biological control; cotton; maize; risk assessment ID BT TRANSGENIC PLANTS; COTTON; HETEROPTERAN; LEPIDOPTERA; PREDATORS; BEHAVIOR; MAIZE; CROPS; IPM AB Cotton- and maize-producing insecticidal crystal (Cry) proteins from the bacterium, Bacillus thuringiensis (Bt), have been commercialized since 1996. Bt plants are subjected to environmental risk assessments for non-target organisms, including natural enemies that suppress pest populations. Here, we used Cry1F-resistant Spodoptera frugiperda (J.E. Smith) and Cry1Ac and Cry2Ab-resistant Trichoplusia ni (Hubner) as prey for the assassin bug, Zelus renardii (Kolenati), a common predator in maize and cotton fields. In tritrophic studies, we assessed several fitness parameters of Z.renardii when it fed on resistant S.frugiperda that had fed on Bt maize expressing Cry1F or on resistant T.ni that had fed on Bt cotton expressing Cry1Ac and Cry2Ab. Survival, nymphal duration, adult weight, adult longevity and female fecundity of Z.renardii were not different when they were fed resistant-prey larvae (S.frugiperda or T.ni) reared on either a Bt crop or respective non-Bt crops. ELISA tests demonstrated that the Cry proteins were present in the plant at the highest levels, at lower levels in the prey and at the lowest levels in the predator. While Z.renardii was exposed to Cry1F and Cry1Ac and Cry2Ab when it fed on hosts that consumed Bt-transgenic plants, the proteins did not affect important fitness parameters in this common and important predator. C1 [Su, H. -H.; Tian, J. -C.; Shelton, A. M.] Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. [Su, H. -H.] Yangzhou Univ, Sch Hort & Plant Protect, Yangzhou, Peoples R China. [Tian, J. -C.] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China. [Naranjo, S. E.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Romeis, J.] Inst Sustainabil Sci ISS, Zurich, Switzerland. [Hellmich, R. L.] Iowa State Univ, ARS, USDA, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Hellmich, R. L.] Iowa State Univ, Dept Entomol, Ames, IA USA. RP Shelton, AM (reprint author), Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. EM ams5@cornell.edu FU Biotechnology Risk Assessment Program Competitive Grant from the USDA, National Institute of Food and Agriculture [2010-33522-21772] FX This project was supported by the Biotechnology Risk Assessment Program Competitive Grant No. 2010-33522-21772 from the USDA, National Institute of Food and Agriculture. The technical help and editing assistance of H. L. Collins is gratefully acknowledged. We are grateful to P. Wang for supplying T. ni. The authors declare no conflict of interest for this study. NR 32 TC 1 Z9 1 U1 4 U2 35 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2015 VL 139 IS 1-2 BP 23 EP 30 DI 10.1111/jen.12184 PG 8 WC Entomology SC Entomology GA AY6YS UT WOS:000347709200003 ER PT J AU Tinsley, NA Spencer, JL Estes, RE Prasifka, JR Schrader, PM French, BW Gray, ME AF Tinsley, N. A. Spencer, J. L. Estes, R. E. Prasifka, J. R. Schrader, P. M. French, B. W. Gray, M. E. TI Larval mortality and development for rotation-resistant and rotation-susceptible populations of western corn rootworm on Bt corn SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Bacillus thuringiensis Berliner; Diabrotica virgifera virgifera LeConte; Zea mays (L; ) ID VIRGIFERA-VIRGIFERA COLEOPTERA; CRY34/35AB1 PROTEINS; MAIZE HYBRIDS; CHRYSOMELIDAE; FIELD; INJURY; NEBRASKA; DAMAGE; INSECTICIDE; EXPRESSION AB The western corn rootworm, Diabrotica virgifera virgifera LeConte, is one of the most economically important insect pests threatening the production of corn, Zea mays (L.), in the United States. Throughout its history, this insect has displayed considerable adaptability by overcoming a variety of pest management tactics, including the cultural practice of annual crop rotation. Since first reported in Illinois in the late 1980s, populations of the rotation-resistant western corn rootworm have spread over a wide area of the eastern Corn Belt. Currently, little information is available concerning the interaction of rotation resistance with the use of genetically modified corn expressing insecticidal toxins from Bacillus thuringiensis Berliner (Bt), a popular tactic for preventing larval injury and its associated yield loss. The goal of this greenhouse experiment was to determine whether rotation-resistant and rotation-susceptible western corn rootworm larvae differ with respect to survival or development when exposed to single- or dual-toxin (pyramided) Bt corn. Individual corn plants were infested with 225 near-hatch eggs at the V5 (five leaf collar) growth stage. Larvae developed undisturbed on the root systems for 17days, after which they were recovered using Berlese-Tullgren funnels. Surviving larvae were counted to estimate mortality, and head capsule widths were measured to assess development. Rotation-resistant and rotation-susceptible larvae had statistically similar mean levels of mortality and head capsule widths when exposed to both single-toxin (Cry3Bb1 or Cry34/35Ab1) and pyramided (Cry3Bb1+ Cry34/35Ab1) Bt corn, suggesting that these two populations do not differ with respect to survival or development when exposed to Bt corn. Additionally, the statistically similar mean levels of mortality for larvae exposed to single-toxin and pyramided Bt corn suggest that pyramided Bt hybrids containing the Cry3Bb1 and Cry34/35Ab1 toxins do not result in additive mortality for western corn rootworm larvae. Implications for management of this economically important pest are discussed. C1 [Tinsley, N. A.; Estes, R. E.; Schrader, P. M.; Gray, M. E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Spencer, J. L.] Univ Illinois, Illinois Nat Hist Survey, Prairie Res Inst, Champaign, IL 61820 USA. [Prasifka, J. R.] ARS, USDA, No Crop Sci Lab, Fargo, ND USA. [French, B. W.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD USA. RP Tinsley, NA (reprint author), Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM tinsley@illinois.edu RI Spencer, Joseph/B-6876-2012 OI Spencer, Joseph/0000-0003-4757-563X FU Insect Management and Insecticide Evaluation Program of the University of Illinois; Pioneer Crop Management Research Awards Program (DuPont Pioneer) FX We thank everyone who assisted with trial establishment and maintenance, including Caitlin Allen, Shane Bailey, Daniel Fulton and Andrew Morehouse. We extend our gratitude to Chad Nielson of the USDA ARS North Central Agricultural Research Laboratory for coordinating the delivery of western corn rootworm eggs used for this experiment. We thank the helpful personnel of the Turner Hall greenhouse and Natural Resources Studies Annex greenhouse. Seed was provided by Monsanto Co. and DuPont Pioneer. Funding for this experiment was provided by the Insect Management and Insecticide Evaluation Program of the University of Illinois and the Pioneer Crop Management Research Awards Program (DuPont Pioneer). NR 38 TC 1 Z9 1 U1 3 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2015 VL 139 IS 1-2 BP 46 EP 54 DI 10.1111/jen.12149 PG 9 WC Entomology SC Entomology GA AY6YS UT WOS:000347709200005 ER PT J AU Cha, DH Gill, MA Epsky, ND Werle, CT Adamczyk, JJ Landolt, PJ AF Cha, D. H. Gill, M. A. Epsky, N. D. Werle, C. T. Adamczyk, J. J. Landolt, P. J. TI From a non-target to a target: identification of a fermentation volatile blend attractive to Zaprionus indianus SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE African fig fly; chemical attractant; Drosophilidae; invasive insect; non-target insect; trap ID SUZUKII DIPTERA DROSOPHILIDAE; VINEGAR; FUNGI; WINE AB The African fig fly, Zaprionus indianus Gupta, is rapidly spreading through the New World and is a new potential pest for numerous fruit crops. Methods are needed to detect and monitor Z.indianus. A recent study shows that Z.indianus can be attracted with a mixture of wine and vinegar, but there are no chemical attractants yet identified. This fly was captured incidentally as a non-target insect in experiments to develop chemical lures, based on wine and vinegar fermentation volatiles, for Drosophila suzukii Matsumura and Drosophilamelanogaster Meigen. We then generated testable hypotheses on what combination of these volatiles was involved in Z.indianus attraction to wine and vinegar. We determined through a series of trapping experiments that the blend of ethanol, acetic acid, acetoin, isoamyl acetate, methionol and ethyl hexanoate constitutes a strong attractant for Z.indianus and accounts for its attraction to the combination of wine and vinegar. These results and findings provide the first opportunity to develop a long-lasting and consistent chemical lure for trapping of Z.indianus. Such a lure in a suitable trap should provide a good means to document the spread of the fly and determine its seasonality and abundance in new areas and crops. C1 [Cha, D. H.; Landolt, P. J.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Gill, M. A.; Epsky, N. D.] ARS, Subtrop Hort Res Stn, USDA, Miami, FL USA. [Werle, C. T.; Adamczyk, J. J.] ARS, Thad Cochran Southern Hort Res Lab, USDA, Poplarville, MS USA. RP Cha, DH (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM dong.cha@ars.usda.gov FU Washington Tree Fruit Research Commission FX This work was supported in part by funding from the Washington Tree Fruit Research Commission. Technical assistance was provided by Daryl Green (USDA/ARS, Wapato, WA), Teri Allen, Erick Rodriguez (USDA/ARS, Miami, FL) and Rogelio de la Rosa (St. Thomas Univ., Miami, FL). Rufus Isaacs, Robert Meagher Jr., Wee Yee and two anonymous reviewers provided insightful suggestions for improvement in the manuscript. We thank Luis Monterde for use of his blueberry farm and Jonathan Crane (Univ. of FL-TREC, Homestead, FL) for access to the guava plantings. NR 23 TC 2 Z9 2 U1 1 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD FEB PY 2015 VL 139 IS 1-2 BP 114 EP 122 DI 10.1111/jen.12164 PG 9 WC Entomology SC Entomology GA AY6YS UT WOS:000347709200012 ER PT J AU Wallis, CM Rashed, A Chen, J Paetzold, L Workneh, F Rush, CM AF Wallis, C. M. Rashed, A. Chen, J. Paetzold, L. Workneh, F. Rush, C. M. TI Effects of Potato-Psyllid-Vectored 'Candidatus Liberibacter solanacearum' Infection on Potato Leaf and Stem Physiology SO PHYTOPATHOLOGY LA English DT Article DE carbohydrates ID ZEBRA CHIP DISEASE; SECONDARY METABOLITES; PHENOLIC-COMPOUNDS; UNITED-STATES; CELL-DEATH; 1ST REPORT; TUBERS; DEFENSE; RESISTANCE; EXPRESSION AB The bacterium 'Candidatus Liberibacter solanacearum' is associated with zebra chip disease (ZC), a threat to potato production in North America and New Zealand. It is vectored by potato psyllids. Previous studies observed that 'Ca. L. solanacearum' infection causes potato tubers to undergo ZC-symptom-associated shifts in physiology, such as increased levels of amino acids, sugars, and phenolics. However, little is known about how 'Ca. L. solanacearum' infections caused by psyllid vector feeding may affect metabolism in potato foliage and stems. This study compared metabolism in potato plants fed upon by 'Ca. L. solanacearum'-positive psyllids with potato plants not exposed to psyllids. Foliar levels of asparagine, aspartic acid, glutamine, fructose, glucose, sucrose, a ferulic acid derivative, and quinic acid were lower in 'Ca. L. solanacearum'-inoculated than noninfected plants. However, foliar levels of proline, serine, four phenolic compounds, and most terpenoids were greater in 'Ca. L. solanacearum'-inoculated than noninfected plants. Upper stem levels of asparagine and aspartic acid, upper and lower stem levels of ellagitannins and most monoterpenoids, and lower stem level of sesquiterpenoids were greater in 'Ca. L. solanacearum'-inoculated than noninfected plants. These results suggest that many defense-related terpenoid compounds might increase in plants which had psyllids inoculate 'Ca. L. solanacearum'. This could impact progression and spread of ZC. C1 [Wallis, C. M.; Chen, J.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res Unit, Parlier, CA 93648 USA. [Rashed, A.] Univ Idaho, Aberdeen Res & Extens Ctr, Dept Plant Soil & Entomol Sci, Aberdeen, ID 83210 USA. [Paetzold, L.; Workneh, F.; Rush, C. M.] Texas A&M AgriLife Res, Amarillo, TX 79106 USA. RP Wallis, CM (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM christopher.wallis@ars.usda.gov NR 46 TC 6 Z9 6 U1 4 U2 22 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2015 VL 105 IS 2 BP 189 EP 198 DI 10.1094/PHYTO-04-14-0113-R PG 10 WC Plant Sciences SC Plant Sciences GA AZ0SI UT WOS:000347954500004 PM 25469656 ER PT J AU Babiker, EM Gordon, TC Jackson, EW Chao, S Harrison, SA Carson, ML Obert, DE Bonman, JM AF Babiker, Ebrahiem M. Gordon, Tyler C. Jackson, Eric W. Chao, Shiaoman Harrison, Stephen A. Carson, Martin L. Obert, Don E. Bonman, J. Michael TI Quantitative Trait Loci from Two Genotypes of Oat (Avena sativa) Conditioning Resistance to Puccinia coronata SO PHYTOPATHOLOGY LA English DT Article DE oat resistance; Puccinia coronata; single nucleotide polymorphism ID F-SP AVENAE; CROWN RUST; CULTIVATED OAT; IDENTIFICATION; MARKERS; O-301; L. AB Developing oat cultivars with partial resistance to crown rust would be beneficial and cost-effective for disease management. Two recombinant inbred-line populations were generated by crossing the susceptible cultivar Provena with two partially resistant sources, CDC Boyer and breeding line 94197A1-9-2-2-2-5. A third mapping population was generated by crossing the partially resistant sources to validate the quantitative trait locus (QTL) results. The three populations were evaluated for crown rust severity in the field at Louisiana State University (LSU) in 2009 and 2010 and at the Cereal Disease Laboratory (CDL) in St. Paul, MN, in 2009, 2010, and 2011. An iSelect platform assay containing 5,744 oat single nucleotide polymorphisms was used to genotype the populations. From the 2009 CDL test, linkage analyses revealed two QTLs for partial resistance in the Provena/CDC Boyer population on chromosome 19A. One of the 19A QTLs was also detected in the 2009 LSU test. Another QTL was detected on chromosome 12D in the CDL 2009 test. In the Provena/94197A1-9-2-2-2-5 population, only one QTL was detected, on chromosome 13A, in the CDL 2011 test. The 13A QTL from the Provenal 94197A1-9-2-2-2-5 population was validated in the CDC Boyer/94197A19-2-2-2-5 population in the CDL 2010 and 2011 tests. Comparative analysis of the significant marker sequences with the rice genome database revealed 15 candidate genes for disease resistance on chromosomes 4 and 6 of rice. These genes could be potential targets for cloning from the two resistant parents. C1 [Babiker, Ebrahiem M.; Gordon, Tyler C.; Bonman, J. Michael] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Jackson, Eric W.] Gen Mills Inc, Kannapolis, NC 28081 USA. [Chao, Shiaoman] ARS, USDA, Fargo, ND 58102 USA. [Harrison, Stephen A.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Carson, Martin L.] ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Obert, Don E.] Limagrain Cereal Seeds, Battle Ground, IN 47920 USA. RP Bonman, JM (reprint author), ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. EM Mike.Bonman@ars.usda.gov NR 21 TC 3 Z9 3 U1 1 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2015 VL 105 IS 2 BP 239 EP 245 DI 10.1094/PHYTO-04-14-0114-R PG 7 WC Plant Sciences SC Plant Sciences GA AZ0SI UT WOS:000347954500009 PM 25121640 ER PT J AU Del Ponte, EM Spolti, P Ward, TJ Gomes, LB Nicolli, CP Kuhnem, PR Silva, CN Tessmann, DJ AF Del Ponte, Emerson M. Spolti, Pierri Ward, Todd J. Gomes, Larissa B. Nicolli, Camila P. Kuhnem, Paulo R. Silva, Cleiltan N. Tessmann, Dauri J. TI Regional and Field-Specific Factors Affect the Composition of Fusarium Head Blight Pathogens in Subtropical No-Till Wheat Agroecosystem of Brazil SO PHYTOPATHOLOGY LA English DT Article ID GIBBERELLA-ZEAE; TRICHOTHECENE GENOTYPES; SOUTHERN BRAZIL; GENEALOGICAL CONCORDANCE; NIVALENOL CONTAMINATION; GRAMINEARUM COMPLEX; GENETIC DIVERSITY; F-ASIATICUM; DEOXYNIVALENOL; CHEMOTYPES AB A multiyear survey of >200 wheat fields in Parana (PR) and Rio Grande do Sul (RS) states was conducted to assess the extent and distribution of Fusarium graminearum species complex (FGSC) diversity in the southern Brazilian wheat agroecosystem. Five species and three trichothecene genotypes were found among 671 FGSC isolates from Fusarium head blight (FHB)-infected wheat heads: F. graminearum (83%) of the 15-acetyldeoxynivalenol (15-ADON) genotype, F. meridionale (12.8%) and F. asiaticum (0.4%) of the nivalenol (NIV) genotype, and F. cortaderiae (2.5%) and F. austroamericanum (0.9%) with either the NIV or the 3-ADON genotype. Regional differences in FGSC composition were observed, with F. meridionale and the NIV type being significantly (P < 0.001) more prevalent in PR (>28%) than in RS (<= 9%). Within RS, F. graminearum was overrepresented in fields below 600 m in elevation and in fields with higher levels of FHB incidence (P < 0.05). Species composition was not significantly influenced by previous crop or the stage of grain development at sampling. Habitat-specific differences in FGSC composition were evaluated in three fields by characterizing a total of 189 isolates collected from corn stubble, air above the wheat canopy, and symptomatic wheat kernels. Significant differences in FGSC composition were observed among these habitats (P < 0.001). Most strikingly, F. meridionale and F. cortaderiae of the NIV genotype accounted for the vast majority (>96%) of isolates from corn stubble, whereas F. graminearum with the 15-ADON genotype was dominant (>84%) among isolates from diseased wheat kernels. Potential differences in pathogenic fitness on wheat were also suggested by a greenhouse competitiveness assay in which F. graminearum was recovered at much higher frequency (>90%) than F. meridionale from four wheat varieties inoculated with an equal mixture of F graminearum and F. meridionale isolates. Taken together, the data presented here suggest that FGSC composition and, consequently, the trichothecene contamination in wheat grown in southern Brazil is influenced by host adaptation and pathogenic fitness. Evidence that F. meridionale and F. cortaderiae with the NIV genotype are regionally significant contributors to FHB may have significant implications for food safety and the economics of cereal production. C1 [Del Ponte, Emerson M.; Spolti, Pierri; Gomes, Larissa B.; Nicolli, Camila P.; Kuhnem, Paulo R.] Univ Fed Rio Grande do Sul, Dept Fitossanidade, BR-91540000 Porto Alegre, RS, Brazil. [Ward, Todd J.] ARS, USDA, Peoria, IL USA. [Silva, Cleiltan N.; Tessmann, Dauri J.] Univ Estadual Maringa, Dept Agron, Maringa, Parana, Brazil. RP Del Ponte, EM (reprint author), Univ Fed Vicosa, Dept Fitopatol, BR-36570900 Vicosa, MG, Brazil. EM delponte@ufv.br NR 64 TC 20 Z9 20 U1 4 U2 23 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2015 VL 105 IS 2 BP 246 EP 254 DI 10.1094/PHYTO-04-14-0102-R PG 9 WC Plant Sciences SC Plant Sciences GA AZ0SI UT WOS:000347954500010 PM 25121641 ER PT J AU Travadon, R Baumgartner, K AF Travadon, Renaud Baumgartner, Kendra TI Molecular Polymorphism and Phenotypic Diversity in the Eutypa Dieback Pathogen Eutypa lata SO PHYTOPATHOLOGY LA English DT Article ID MULTILOCUS GENOTYPE DATA; POPULATION-STRUCTURE; GRAPEVINE PATHOGEN; GENETIC-STRUCTURE; LOCAL ADAPTATION; DISEASE RISK; SPECIATION; FUNGUS; INDIVIDUALS; SPECIFICITY AB Pathogen adaptation to different hosts can lead to specialization and, when coupled with reproductive isolation, genome-wide differentiation and ecological speciation. We tested the hypothesis of host specialization among California populations of Eutypa lata (causal fungus of Eutypa dieback of grapevine and apricot), which is reported from >90 species. Genetic analyses of nine microsatellite loci in 182 isolates from three hosts (grapevine, apricot, and willow) at three locations were complemented by cross-inoculations on cultivated hosts grapevine and apricot to reveal patterns of host specialization. The cultivated hosts are likely more important sources of inoculum than the wild host willow, based on our findings of higher pathogen prevalence and allelic richness in grapevine and apricot. High levels of gene flow among all three hosts and locations, and no grouping by clustering analyses, suggest neither host nor geographic differentiation. Cross-inoculations revealed diversified phenotypes harboring various performance levels in grapevine and apricot, with no apparent correlation with their host of origin. Such phenotypic diversity may enable this pathogen to persist and reproduce as a generalist. Regular genetic reshuffling through sexual recombination, frequent immigration among hosts, and the lack of habitat choice in this passively dispersed fungus may prevent fixation of alleles controlling host specialization. C1 [Travadon, Renaud] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Baumgartner, Kendra] ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Baumgartner, K (reprint author), ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. EM kbaumgartner@ucdavis.edu NR 44 TC 3 Z9 3 U1 2 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2015 VL 105 IS 2 BP 255 EP 264 DI 10.1094/PHYTO-04-14-0117-R PG 10 WC Plant Sciences SC Plant Sciences GA AZ0SI UT WOS:000347954500011 PM 25084304 ER PT J AU Miles, TD Martin, FN Coffey, MD AF Miles, Timothy D. Martin, Frank N. Coffey, Michael D. TI Development of Rapid Isothermal Amplification Assays for Detection of Phytophthora spp. in Plant Tissue SO PHYTOPATHOLOGY LA English DT Article DE sudden oak death ID SUDDEN OAK DEATH; MULTILOCUS PHYLOGENY; MOLECULAR-DETECTION; NATURAL ECOSYSTEMS; CAUSAL AGENT; RAMORUM; PCR; IDENTIFICATION; MITOCHONDRIAL; AUSTRALIA AB Several isothermal amplification techniques recently have been developed that are tolerant of inhibitors present in many plant extracts, which can reduce the need for obtaining purified DNA for running diagnostic assays. One such commercially available technique that has similarities with real-time polymerase chain reaction (PCR) for designing primers and a labeled probe is recombinase polymerase amplification (RPA). This technology was used to develop two simple and rapid approaches for detection of Phytophthora spp.: one genus-specific assay multiplexed with a plant internal control and the other species-specific assays for Phytophthora ramorum and P. kernoviae. All assays were tested for sensitivity (ranging from 3 ng to 1 fg of DNA) and specificity using DNA extracted from more than 136 Phytophthora taxa, 21 Pythium spp., 1 Phytopythium sp., and a wide range of plant species. The lower limit of linear detection using purified DNA was 200 to 300 fg of DNA in all pathogen RPA assays. Six different extraction buffers were tested for use during plant tissue maceration and the assays were validated in the field by collecting 222 symptomatic plant samples from over 50 different hosts. Only 56 samples were culture positive for Phytophthora spp. whereas 91 were positive using the Phytophthora genus-specific RPA test and a TaqMan real-time PCR assay. A technique for the generation of sequencing templates from positive RPA amplifications to confirm species identification was also developed. These RPA assays have added benefits over traditional technologies because they are rapid (results can be obtained in as little as 15 min), do not require DNA extraction or extensive training to complete, use less expensive portable equipment than PCR-based assays, and are significantly more specific than current immunologically based methods. This should provide a rapid, field-deployable capability for pathogen detection that will facilitate point-of-sample collection processing, thereby reducing the time necessary for accurate diagnostics and making management decisions. C1 [Miles, Timothy D.; Martin, Frank N.] ARS, USDA, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. [Coffey, Michael D.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. RP Martin, FN (reprint author), ARS, USDA, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. EM frank.martin@ars.usda.gov NR 35 TC 2 Z9 3 U1 6 U2 42 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD FEB PY 2015 VL 105 IS 2 BP 265 EP 278 DI 10.1094/PHYTO-05-14-0134-R PG 14 WC Plant Sciences SC Plant Sciences GA AZ0SI UT WOS:000347954500012 PM 25208239 ER PT J AU Bowzer, J Trushenski, J Rawles, S Gaylord, TG Barrows, FT AF Bowzer, J. Trushenski, J. Rawles, S. Gaylord, T. G. Barrows, F. T. TI Apparent digestibility of Asian carp- and common carp-derived fish meals in feeds for hybrid striped bass Morone saxatilis female x M. chrysops male and rainbow trout Oncorhynchus mykiss SO AQUACULTURE NUTRITION LA English DT Article DE bighead carps; fishmeal; Hypophthalmichthys spp; invasive species; sunshine bass ID FATTY-ACID-COMPOSITION; BY-PRODUCT MEAL; ANIMAL PROTEIN INGREDIENTS; FECAL COLLECTION METHODS; SUNSHINE BASS; GROWTH-PERFORMANCE; SEASONAL-CHANGES; FED DIETS; POULTRY; AVAILABILITY AB Apparent digestibility coefficients (ADCs) of nutrients (crude protein, amino acids, crude lipid, fatty acids, and minerals) were determined for fish meals derived from menhaden, Asian carp (combination of silver and bighead carps), and common carp in feeds for hybrid striped bass and rainbow trout. Extruded test diets were formulated to contain a 70:30 mixture of reference diet and test ingredient with yttrium oxide (1gkg(-1)) serving as the inert marker. Diets were randomly assigned to triplicate tanks and fish were fed once per day at 2% body weight. Fecal samples were collected by manual stripping. The ADCs were calculated according to standard procedures. The composition and digestibility of Asian carp and common carp meals was broadly similar to menhaden meal. Protein digestibility ranged from 86.5% (Asian carp meal) to 93.1% (common carp meal). Lipid was highly digestible with ADCs >100% for all ingredients. Although the Asian carp meal was less digestible than the other two fish meals, it was still a highly digestible ingredient. Our data suggest that fish meals derived from Asian or common carp would be valuable feedstuffs in diets for hybrid striped bass, rainbow trout, and possibly other cultured fishes. C1 [Bowzer, J.; Trushenski, J.] So Illinois Univ, Ctr Fisheries Aquaculture & Aquat Sci, Carbondale, IL 62901 USA. [Rawles, S.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR USA. [Gaylord, T. G.] US Fish & Wildlife Serv, Bozeman Fish Technol Ctr, Bozeman, MT USA. [Barrows, F. T.] ARS, USDA, Bozeman Fish Technol Ctr, Bozeman, MT USA. RP Trushenski, J (reprint author), So Illinois Univ, Ctr Fisheries Aquaculture & Aquat Sci, Carbondale, IL 62901 USA. EM saluski@siu.edu FU Illinois Department of Natural Resources FX The authors thank the Illinois Department of Natural Resources for financial support of the work described herein. We also thank Chris Bowzer, Andrew Ciuris, and Matthew Young for assistance in data collection and processing, and David Glover for guidance regarding statistical analysis. NR 65 TC 2 Z9 2 U1 6 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD FEB PY 2015 VL 21 IS 1 BP 43 EP 53 DI 10.1111/anu.12136 PG 11 WC Fisheries SC Fisheries GA AY2WC UT WOS:000347446000005 ER PT J AU Klein, LR Hendrix, WG Lohr, VI Kaytes, JB Sayler, RD Swanson, ME Elliot, WJ Reganold, JP AF Klein, Linda R. Hendrix, William G. Lohr, Virginia I. Kaytes, Jolie B. Sayler, Rodney D. Swanson, Mark E. Elliot, William J. Reganold, John P. TI Linking ecology and aesthetics in sustainable agricultural landscapes: Lessons from the Palouse region of Washington, USA SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Agricultural landscape ecology; Conservation buffer system; Ecosystem services; GeoWEPP modeling; Landscape preference; Multifunctional landscapes ID RURAL LANDSCAPES; VISUAL QUALITY; SCENIC BEAUTY; UNITED-STATES; CONSERVATION; BIODIVERSITY; MANAGEMENT; PREFERENCE; BUFFERS; WILDERNESS AB Inspired by international escalation in agricultural sustainability debates, we explored the promise of landscape-scale conservation buffers to mitigate environmental damage, improve ecological function, and enhance scenic quality. Although the ecological benefits of buffer vegetation are well established by plot- and field-scale research, buffer adoption by farmers is limited. Landscape-scale approaches can address several obstacles by simultaneously considering ecological impact, economic efficiency, and aesthetic quality and preference in buffer placement and design. Within four watersheds of Washington's Palouse farming region, we examined relationships between ecological and aesthetic responses to the existing landscape structure plus three alternative scenarios, differentiated by successive increases in woody buffers. Methodology combined GIS analysis, digital image simulation, soil erosion modeling and mapping, and a landscape preference survey. Landscape ecological function, measured by erosion and deposition rates, improved as buffer elements were added into each successive scenario. Magnitude of improvements varied among scenarios and among watersheds, revealing opportunities for targeting buffers to maximize ecological benefits and economic efficiency. Concurrently, aesthetic preference, measured as scenic quality ratings, increased significantly (p < 0.05) from the existing landscape through the second successive scenario of improved ecological function. No preference difference was found between the second and third scenarios. Results expand current understanding of multifunctional relationships in agricultural landscapes and encourage future research on whether linking ecological and aesthetic quality in buffer design might favorably influence adoption. Results also suggest that, within certain landscape contexts, visually perceivable attributes can provide a relative and coincident indication of ecological function, aesthetic quality, and agricultural sustainability. (C).) 2014 Elsevier BM. All rights reserved. C1 [Klein, Linda R.] Washington State Univ, Coll Arts & Sci, Sch Environm, Pullman, WA 99164 USA. [Hendrix, William G.; Kaytes, Jolie B.] Washington State Univ, Sch Design & Construct, Pullman, WA 99164 USA. [Lohr, Virginia I.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Sayler, Rodney D.; Swanson, Mark E.] Washington State Univ, Coll Agr Human & Nat Resource Sci, Sch Environm, Pullman, WA 99164 USA. [Elliot, William J.] USDA Forest Serv, Moscow Forestry Sci Lab, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Reganold, John P.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Klein, LR (reprint author), LRK Consulting, Writing Editing & Project Coordinat, 440 SE Hill St, Pullman, WA 99163 USA. EM linda.klein@wsu.edu; whendrix@wsu.edu; lohr@wsu.edu; jolie@wsu.edu; rdsayler@wsu.edu; markswanson@wsu.edu; welliot@fs.fed.us; reganold@wsu.edu FU Natural Resource Conservation Endowment Fund FX Many thanks to the anonymous participants in Whitman County, WA for completing the landscape preference survey. We gratefully acknowledge Washington State University's Social and Economic Sciences Research Center for their guidance in survey design, sampling protocol, and implementation. Much appreciation is also extended to Martin Minkowski, Ph.D. (ESRI, Redlands, CA), Ina Sue Miller (Moscow Forestry Sciences Laboratory, Rocky Mountain Research Station, USDA-USFS, Moscow, ID), and Dennis C. Flanagan, Ph.D., and Jim Frankenberger (both from National Soil Erosion Research Laboratory, USDA-ARS, West Lafayette, IN) for their expert assistance with the GeoWEPP model. We also especially thank the journal's anonymous reviewers for their time and thoughtful critique and suggestions, all of which greatly improved this manuscript. Part of this research was funded by a grant from the Natural Resource Conservation Endowment Fund, established by Jane P. Conrad, and entrusted to Washington State University. NR 85 TC 6 Z9 6 U1 16 U2 74 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD FEB PY 2015 VL 134 BP 195 EP 209 DI 10.1016/j.landurbplan.2014.10.019 PG 15 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA AY3WY UT WOS:000347511400020 ER PT J AU Pratt, SL Stowe, HM Whitlock, BK Strickland, L Miller, M Calcatera, SM Dimmick, MD Aiken, GE Schrick, FN Long, NM Duckett, SK Andrae, JG AF Pratt, S. L. Stowe, H. M. Whitlock, B. K. Strickland, L. Miller, M. Calcatera, S. M. Dimmick, M. D. Aiken, G. E. Schrick, F. N. Long, N. M. Duckett, S. K. Andrae, J. G. TI Bulls grazing Kentucky 31 tall fescue exhibit impaired growth, semen quality, and decreased semen freezing potential SO THERIOGENOLOGY LA English DT Article DE Computerized sperm-quality analysis; Scrotal circumference; Fescue toxicosis; Breeding soundness examination; Semen freezing ID BREEDING SOUNDNESS EVALUATION; YEARLING BEEF BULLS; ENDOPHYTE; PROLACTIN; COWS; STEROIDOGENESIS; EXPRESSION; FERTILITY; TOXICOSIS; RESPONSES AB Serum prolactin (PRL) and testosterone concentrations, body weight, body composition, semen quality, and semen freezing potential for bulls grazing the toxic tall fescue (Lolium arundinaceum [Schreb.] Darbysh. = Schedonorous arundinaceum [Schreb.] Dumort.) cultivar Kentucky 31 (E+) compared with a novel endophyte cultivar lacking ergot alkaloids (E) were evaluated. Angus bulls were allotted to treatment (Day 0) and grazed E+ or E- for 155 days. Treatment-by-day interaction was significant (P < 0.05) for serum PRL concentrations with E+treated bulls exhibiting reduced PRL values compared with E control bulls, but no differences were observed for serum testosterone concentrations (P > 0.05). Further, bulls on the E+ treatment exhibited decreased total gain, average daily gain, and body weight by Day 140 (P < 0.05) compared with the E-bulls. Rump muscle depth was lower because the treatment in bulls grazing E+ compared with E-(P < 0.05) and intramuscular fat in the E-bulls compared with the E+ group was higher by Day 155 (P < 0.05). Analysis of ejaculates showed significant treatment x day effects for sperm concentration with lower values observed for bulls on the E+ treatment (P < 0.05). The percent normal morphology was reduced in ejaculates from E+ bulls compared with E-bulls (P < 0.05), and the difference was due to an increase in abnormal sperm present in the E+ ejaculates from Day 84 to 140 (P < 0.05). In addition, spermatozoa motility and progressive motility were decreased on thawing in semen samples from E+ bulls compared with E- bulls (P < 0.05). (C) 2015 Elsevier Inc. All rights reserved. C1 [Pratt, S. L.; Stowe, H. M.; Miller, M.; Calcatera, S. M.; Dimmick, M. D.; Long, N. M.; Duckett, S. K.; Andrae, J. G.] Clemson Univ, Dept Anim & Vet Sci, Clemson, SC 29634 USA. [Whitlock, B. K.] Univ Tennessee, Coll Vet Med, Knoxville, TN USA. [Strickland, L.; Schrick, F. N.] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37901 USA. [Aiken, G. E.] USDA ARS, Forage Anim Prod Res Unit, Lexington, KY USA. RP Pratt, SL (reprint author), Clemson Univ, Dept Anim & Vet Sci, Clemson, SC 29634 USA. EM scottp@clemson.edu RI Whitlock, Brian/H-4198-2016 OI Whitlock, Brian/0000-0001-7247-0982 FU National Institute of Food and Agriculture/United States Department of Agriculture [SC-1700376]; USDA National Institute of Food and Agriculture [2010-38942-20745]; Cooperative State Research, Education, and Extension Service FX This material is based on work supported by National Institute of Food and Agriculture/United States Department of Agriculture, under project number SC-1700376 and was supported by National Research Initiative Competitive grant number 2010-38942-20745 from the USDA National Institute of Food and Agriculture and the Cooperative State Research, Education, and Extension Service and has been assigned Technical Contribution number 6194 of the Clemson University Experiment Station. The author would also like to thank Johanna E. Johnson with assistance in preparing this article. NR 25 TC 3 Z9 3 U1 1 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0093-691X EI 1879-3231 J9 THERIOGENOLOGY JI Theriogenology PD FEB PY 2015 VL 83 IS 3 BP 408 EP 414 DI 10.1016/j.theriogenology.2014.10.001 PG 7 WC Reproductive Biology; Veterinary Sciences SC Reproductive Biology; Veterinary Sciences GA AY3SA UT WOS:000347501600014 PM 25459421 ER PT J AU Carrillo, D Dunlap, CA Avery, PB Navarrete, J Duncan, RE Jackson, MA Behle, RW Cave, RD Crane, J Rooney, AP Pena, JE AF Carrillo, D. Dunlap, C. A. Avery, P. B. Navarrete, J. Duncan, R. E. Jackson, M. A. Behle, R. W. Cave, R. D. Crane, J. Rooney, A. P. Pena, J. E. TI Entomopathogenic fungi as biological control agents for the vector of the laurel wilt disease, the redbay ambrosia beetle, Xyleborus glabratus (Coleoptera: Curculionidae) SO BIOLOGICAL CONTROL LA English DT Article DE Avocado; Lauraceae; Xyleborus glabratus; Invasive species; Beauveria bassiana; Isaria fumosorosea ID SOUTHEASTERN UNITED-STATES; ANISOPLIAE VAR. ACRIDUM; BEAUVERIA-BASSIANA; METARHIZIUM-ANISOPLIAE; RAFFAELEA-LAURICOLA; SURFACE-PROPERTIES; SCOLYTINAE; AVOCADO; FLORIDA; BLASTOSPORES AB The redbay ambrosia beetle (RAB), Xyleborus glabratus, is a wood-boring insect that vectors the fungal pathogen, Raffaelea lauricola, which causes laurel wilt, a lethal disease of avocado. The objective of this study was to determine the susceptibility of RAB to infection and subsequent death by exposure to three commercial strains of entomopathogenic fungi [two strains of Isaria fumosorosea (Ifr 3581 and PFR), and strain GHA of Beauveria bassiana]. RAB females were dipped in fungal spore solutions and their median survivorship times (MST) determined. Contact with any of the biopesticides resulted in death of all RAB females. MSTs of RAB females ranged from 3 days (B. bassiana) to 5 days (I. fumosorosea PFR). B. bassiana killed RAB females faster, followed by Ifr 3581 and PFR. RAB females dipped in B. bassiana suspensions had the highest number of viable spores attached to their bodies, followed by Ifr 3581. Beetles dipped in PFR suspension had significantly less viable spores attached to their bodies. No significant differences were observed in the mortality of beetles exposed to entomopathogenic fungi by dipping in a fungal suspension or walking on treated avocado bolts. Beetles bored into the logs and constructed galleries, but they were found dead inside the galleries a few days after exposure to the entomopathogens. Entomopathogenic fungal infection in dead beetles was confirmed through molecular techniques. This is the first study to demonstrate that entomopathogenic fungi are potential biological control agents against RAB. (C) 2014 Elsevier Inc. All rights reserved. C1 [Carrillo, D.; Navarrete, J.; Duncan, R. E.; Crane, J.; Pena, J. E.] Univ Florida, Trop Res & Educ Ctr, Homestead, FL 33031 USA. [Dunlap, C. A.; Jackson, M. A.; Behle, R. W.; Rooney, A. P.] USDA, Agr Res Serv, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. [Avery, P. B.; Cave, R. D.] Univ Florida, Indian River Res & Educ Ctr, Ft Pierce, FL 34945 USA. RP Carrillo, D (reprint author), Univ Florida, Trop Res & Educ Ctr, Homestead, FL 33031 USA. EM dancar@ufl.edu OI Navarrete, Bernardo/0000-0001-9200-7119 FU IR4 Biopesticides; Organic Support grant; USDA-APHIS Farm bill grant FX This research was partially funded by a IR4 Biopesticides and Organic Support grant and a USDA-APHIS Farm bill grant to D. Carrillo and J.E. Pena. NR 53 TC 2 Z9 2 U1 4 U2 82 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD FEB PY 2015 VL 81 BP 44 EP 50 DI 10.1016/j.biocontrol.2014.10.009 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AX3PU UT WOS:000346852200007 ER PT J AU Berner, D Cavin, C Woudenberg, JHC Tunali, B Buyuk, O Kansu, B AF Berner, Dana Cavin, Craig Woudenberg, Joyce H. C. Tunali, Berna Buyuk, Orhan Kansu, Bayram TI Assessment of Boeremia exigua var. rhapontica, as a biological control agent of Russian knapweed (Rhaponticum repens) SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Boeremia exigua var. rhapontica; Rhaponticum repens; Russian knapweed ID LINEAR UNBIASED PREDICTION; CENTAUREA-REPENS; HOST-RANGE; SUBANGUINA-PICRIDIS; BREEDING VALUES; PHOMA-EXIGUA; MODEL; PATHOGENS; SEQUENCES; INFERENCE AB Russian knapweed (Rhaponticum repens (L.) Hidalgo) is an herbaceous perennial weed that was introduced and has become invasive in the United States, particularly in the semi-arid west. It is characterized by its extensive root system, low seed production, and persistence. The weed has caused serious reductions in yields and crop value and may significantly devalue the land itself. Conventional control strategies have been inadequate because of the size of infestations and economic and environmental costs of control. Biological control has been a sought-after potential solution to this weed problem. In the summer of 2002, diseased R. repens plants were collected near Cankiri, Turkey, and the facultative saprophytic fungus Boeremia exigua isolate FDWSRU 02-059 was isolated from diseased plants. Bayesian analysis of the actin, beta-tubulin, calmodulin, elongation factor, and ITS genes, of 66 isolates, representing the ten species of Boeremia and the 11 varieties of B. exigua, including FDWSRU 02-059, showed that the isolate is a unique genetic entity and was named B. exigua var. rhapontica Berner, Woudenberg & Tunali, var. nov. MycoBank MB809363. Disease incidence and severity data from host-range determination tests conducted at 25 degrees C, the optimum temperature for growth and sporulation of B. ex. rhapontica, with adequate dew periods, were combined with a genetic distance matrix based on ITS sequences of 66 plant species related to R. repens. The combined disease and genetic data were analyzed by mixed model equations to produce best linear unbiased predictors (BLUPs), standard errors, and P > |t| values, in t-tests against zero, for disease incidence and severity for each species. BLUPs of disease incidence were significantly different from zero only for three Rhaponticum spp. while BLUPs of disease severity rankings were significantly different from zero only for R. repens, Rhaponticum carthamoides, Rhaponticum uniflorum, and Leuzea berardioides. Best linear unbiased predictors for differences in above-ground dry weights between control and inoculated plants of a subset of the species evaluated were not significant. However, above-ground damage by B. ex. rhapontica to R. repens was nearly twice that for any other species, except Rhaponticum species. Published by Elsevier Inc. C1 [Berner, Dana; Cavin, Craig] USDA, ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Woudenberg, Joyce H. C.] CBS, KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands. [Tunali, Berna; Kansu, Bayram] Ondokuz Mayis Univ, Fac Agr, Dept Plant Protect, TR-55139 Atakum, Samsun, Turkey. [Buyuk, Orhan] Plant Protect Cent Res Inst, TR-06172 Ankara, Turkey. RP Berner, D (reprint author), USDA, ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM dana.berner@ars.usda.gov RI Woudenberg, Joyce H.C./C-9384-2013 OI Woudenberg, Joyce H.C./0000-0002-7337-6864 FU USDA, ARS Specific Cooperative Agreement [58-1920-1-158F]; Ondokuz Mayis University FX Research in Turkey was supported in part by USDA, ARS Specific Cooperative Agreement #58-1920-1-158F titled "Protocol between Ondokuz Mayis University and the USDA/ARS/FDWSRU on Biological Control of Weeds with Plant Pathogens''. The authors thank Dr. William Bruckart III for comments on the manuscript. NR 58 TC 4 Z9 4 U1 3 U2 24 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD FEB PY 2015 VL 81 BP 65 EP 75 DI 10.1016/j.biocontrol.2014.11.009 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AX3PU UT WOS:000346852200010 ER PT J AU Belle, AJ Lansing, S Mulbry, W Weil, RR AF Belle, Ashley J. Lansing, Stephanie Mulbry, Walter Weil, Ray R. TI Anaerobic co-digestion of forage radish and dairy manure in complete mix digesters SO BIORESOURCE TECHNOLOGY LA English DT Article DE Anaerobic digestion; Cover crops; Biogas; Hydrogen sulfide; Methane ID BIOGAS PRODUCTION; FOOD WASTE; SLURRY AB Pilot-scale digesters (850 L) were used to quantify CH4 and H2S production when using forage radish cover crops as a co-digestion feedstock in dairy manure-based digesters. During two trials, triplicate mixed digesters were operated in batch mode with manure-only or radish + manure (27% and 13% radish by wet weight in Trial 1 and 2, respectively). Co-digestion increased CH4 production by 11% and 39% in Trial 1 and 2, respectively. As H2S production rapidly declined in the radish + manure digesters, CH4 production increased reaching high levels of CH4 (>= 67%) in the biogas. Over time, radish co-digestion lowered the H2S concentration in the biogas (0.20%) beyond that of manure-only digestion (0.34-0.40%), although cumulative H2S production in the radish + manure digesters was higher than manure-only. Extrapolated to a farm-scale (200 cows) continuous mixed digester, co-digesting with radish could generate 3150 m(3) CH4/month, providing a farmer additional revenue up to $3125/month in electricity sales. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Belle, Ashley J.; Lansing, Stephanie; Weil, Ray R.] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. [Mulbry, Walter] USDA ARS, Environm Management & Byprod Lab, Beltsville, MD 20705 USA. RP Lansing, S (reprint author), Univ Maryland, Dept Environm Sci & Technol, 1449 Anim Sci,Ag Engn Bldg, College Pk, MD 20742 USA. EM slansing@umd.edu FU Northeast Sun Grant Initiative [NE10-040] FX This research was supported by the Northeast Sun Grant Initiative (NE10-040). Our sincere appreciation is extended to the University of Maryland's (UMD) Waste to Energy Laboratory, especially Veronika Zhiteneva, Holly Bowen, Brent Murrell, Isabel Enerson, Freddy Witarsa, James Thomas, and Angela Perantoni. We would like to thank Gary Seibel and the UMD Environmental Science and Technology Project Development Center for digester fabrication assistance. Special thanks are also extended to the USDA personnel, especially Shannon Ingram, John Meisinger, and the BARC dairy farm crew. NR 34 TC 6 Z9 6 U1 5 U2 27 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2015 VL 178 BP 230 EP 237 DI 10.1016/j.biortech.2014.09.036 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AX8HM UT WOS:000347150700030 PM 25278111 ER PT J AU Knight, AL Barros-Parada, W Bosch, D Escudero-Colomar, LA Fuentes-Contreras, E Hernandez-Sanchez, J Yung, C Kim, Y Kovanci, OB Levi, A Lo, P Molinari, F Valls, J Gemeno, C AF Knight, A. L. Barros-Parada, W. Bosch, D. Escudero-Colomar, L. A. Fuentes-Contreras, E. Hernandez-Sanchez, J. Yung, C. Kim, Y. Kovanci, O. B. Levi, A. Lo, P. Molinari, F. Valls, J. Gemeno, C. TI Similar worldwide patterns in the sex pheromone signal and response in the oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae) SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE Lepidoptera; sex pheromone; geographical variation; population variation ID BLEND DISCRIMINATION; SYNTHETIC PHEROMONE; AGROTIS-SEGETUM; APPLE ORCHARDS; COMPONENTS; SYSTEM; TRAPS; MALES; ATTRACTIVENESS; SPECIFICITY AB The response of Grapholita molesta (Busck) males to three-component sex pheromone blends containing a 100% ratio of the major sex pheromone component, (Z)-8-dodecenyl acetate and a 10% ratio of (Z)-8-dodecenol, but with varying ratios of (E)-8-dodecenyl acetate (0.4, 5.4, 10.4, 30.4, and 100.1% E-blends) was tested with populations in eight stone and pome fruit orchards in Europe, Asia, and North and South America. Traps baited with the 5.4% E-blend caught significantly more males than traps with any other blend with all populations. Significantly more males were caught in traps baited with the 10.4% E-blend than in traps with the remaining blends, except with the 0.4% E-blend in Turkey. Significant differences in male moth catches occurred between the other blends with the 0.4>30.4% E-blend, and the 30.4>100.1% E-blend. Male moth catches with the 100.1% E-blend only differed from the hexane control in Chile. No apparent differences were noted to these blends in populations collected from pome or stone fruits. Flight tunnel assays to synthetic blends with a subset of populations were similar to the field results, but the breadth of the most attractive E-blends was wider. Flight tunnel assays also demonstrated a high level of male-female cross-attraction among field-collected populations. Female gland extracts from field-collected populations did not show any significant variation in their three-component blends. The only exceptions in these assays were that long-term laboratory populations were less responsive and attractive, and produced different blend ratios of the two minor components than recently collected field populations. C1 [Knight, A. L.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Barros-Parada, W.; Fuentes-Contreras, E.] Univ Talca, Fac Ciencias Agr, Talca, Chile. [Barros-Parada, W.; Fuentes-Contreras, E.] Univ Talca, Talca, Chile. [Bosch, D.] UdL IRTA, Lleida 25198, Spain. [Escudero-Colomar, L. A.] IRTA, Estn Expt Mas Badia, La Tallada Demporda 17134, Girona, Spain. [Hernandez-Sanchez, J.] Queensland Univ Technol, IHBI, Kelvin Grove, Qld 4059, Australia. [Yung, C.; Kim, Y.] Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. [Kovanci, O. B.] Uludag Univ, Fac Agr, Dept Plant Protect, TR-16059 Bursa, Turkey. [Levi, A.; Gemeno, C.] Univ Lleida, Dept Crop & Forest Sci, Lleida 25198, Spain. [Lo, P.] Plant & Food Res, Hawkes Bay 4157, Havelock North, New Zealand. [Molinari, F.] Univ Cattolica Sacro Cuore, Entomol & Plant Pathol Inst, I-29100 Piacenza, Italy. [Valls, J.] Hosp Univ Arnau de Vilanova Lleida HUAV, Inst Recerca Biomed Lleida IRBLLEIDA, Biostat Unit, Lleida 25198, Spain. RP Knight, AL (reprint author), ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov RI Fuentes-Contreras, Eduardo/A-7595-2008; gemeno, cesar/H-5223-2011; Barros, Wilson/I-5535-2013; Bosch, Dolors/A-9405-2015 OI Fuentes-Contreras, Eduardo/0000-0003-3567-3830; gemeno, cesar/0000-0001-5543-6141; Barros, Wilson/0000-0003-3015-4927; Bosch, Dolors/0000-0003-0656-0466 FU MEC, Spain [AGL2010-17486]; Chilean grant ['Conicyt MEC 80120005']; Basic Science Research Program through the National Research Foundation of Korea FX We would like to thank the financial support of MEC, Spain (AGL2010-17486) to C.G., D.B, and L.A.E., and the Chilean grant 'Conicyt MEC 80120005' to C.G. In Girona, Spain, Cesca Alcala with RTAconducted the field experiments and Maria Carbo obtained the research sites. In Turkey, the research assistant Bilgi Pehlevan (Uludag University, Turkey) was very helpful in these studies. In Korea the work was supported by the Basic Science Research Program through the National Research Foundation of Korea. In Italy, Matteo Anaclerio and Manuela Cigolini (Catholic University) were very helpful. Help with the studies conducted in Oregon was provided by Rick Hilton at Oregon State University, Medford, OR. Laboratory colonies from Pennsylvania were provided by Greg Krawczyk, Penn State University, Biglerville, PA, and from California by personnel at the USDA, ARS Laboratory, Parlier, CA. Jean-Claude Tournie, of Prestagro, collected insects in France and shipped them to Spain. NR 54 TC 4 Z9 4 U1 6 U2 26 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD FEB PY 2015 VL 105 IS 1 BP 23 EP 31 DI 10.1017/S0007485314000637 PG 9 WC Entomology SC Entomology GA AX9PJ UT WOS:000347234000003 PM 25234707 ER PT J AU McMurray, JA Roberts, DW Geiser, LH AF McMurray, Jill A. Roberts, David W. Geiser, Linda H. TI Epiphytic lichen indication of nitrogen deposition and climate in the northern rocky mountains, USA SO ECOLOGICAL INDICATORS LA English DT Article DE Climate change; Nitrogen deposition; Lichen; Critical load ID CRITICAL LOADS; MACROLICHEN COMMUNITIES; REACTIVE NITROGEN; WESTERN OREGON; FORESTS; CALIFORNIA; FIELD; GAS; CONSEQUENCES; ECOSYSTEMS AB Lichen bioindication can provide economical and spatially extensive monitoring of climate and pollution impacts on ecological communities. We used non-metric multidimensional scaling of lichen community composition and generalized additive models to analyze regional climate and pollution gradients in the northern Rocky Mountains, U.S. Temperature extremes, relative humidity, and N-deposition were strongly related to lichen community composition. Eutrophic species (genera Physcia, Xanthomendoza, and Xanthoria) were associated with high N deposition, low precipitation, and temperature extremes. Estimated N deposition in our study ranged from <0.5 to 4.26 kg N ha(-1) year(-1) with degradation to lichen communities observed at 4.0 kg N ha(-1) year(-1), the indicated critical load. The resulting model can track changes in climate and N pollution related to lichen communities over time, identify probable sensitive or impacted habitats, and provide key information for natural resource management and conservation. The approach is broadly applicable to temperate ecosystems worldwide. Published by Elsevier Ltd. C1 [McMurray, Jill A.] US Forest Serv, Air Resources Management Program, Bozeman, MT 59711 USA. [Roberts, David W.] Montana State Univ, Dept Ecol, Bozeman, MT 59717 USA. [Geiser, Linda H.] US Forest Serv, Resources Management Program, Pacific Northwest Reg Air, Corvallis, OR USA. RP McMurray, JA (reprint author), US Forest Serv, Air Resources Management Program, Bozeman, MT 59711 USA. EM jamcmurray@fs.fed.us FU USDA Forest Service Rocky Mountain Research Station Forest Inventory and Analysis Program [09-JV-11221638-323] FX We thank Sarah Jovan, Terry Svalberg, Ted Porwoll, Peter Neitlich, and Julie Shea for steadfast support, advice, and resources; Peter Minchin for use of the monoMDS FORTRAN code; Doug Glavich and Bruce McCune for lichen identification; Adam Lunsford, Adam McMurray, Sarah Andresen, and Sommer Roefaro for field assistance, and the University of Minnesota Research Analytical Laboratory for elemental analysis. The USDA Forest Service Rocky Mountain Research Station Forest Inventory and Analysis Program funded this research (Grant #09-JV-11221638-323). NR 61 TC 1 Z9 1 U1 3 U2 65 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD FEB PY 2015 VL 49 BP 154 EP 161 DI 10.1016/j.ecolind.2014.10.015 PG 8 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX5GK UT WOS:000346954500018 ER PT J AU Whittaker, G Barnhart, B Fare, R Grosskopf, S AF Whittaker, Gerald Barnhart, Bradley Faere, Rolf Grosskopf, Shawna TI Application of index number theory to the construction of a water quality index: Aggregated nutrient loadings related to the areal extent of hypoxia in the northern Gulf of Mexico SO ECOLOGICAL INDICATORS LA English DT Article DE Eutrophication; Surface water quality; Gulf hypoxia; Economic productivity; Distance functions ID BIOTIC INDEXES; ENVIRONMENTAL PERFORMANCE; RIVER; MACROINVERTEBRATE; EUTROPHICATION; MODELS; STRATIFICATION; COMMUNITIES; EFFICIENCY; ECOSYSTEMS AB Numerous studies have linked individual nutrient loadings from the Mississippi and Atchafalaya Rivers to the growth of the hypoxic, or oxygen depleted, zone in the northern Gulf of Mexico. However, in the discussion of policy to remediate Gulf hypoxia, it is beneficial for stakeholders and policymakers to obtain a single measure for water quality that characterizes information from multiple water pollutants. This study aggregates loadings from six nutrients measured at the entrance to the Gulf of Mexico into a single time-varying index of water quality. The index is constructed using traditional index number theory originating from economic production theory, mainly, Shephard's distance functions calculated using data envelopment analysis (DEA). The methodology is an advance over other index construction schemes because the determined metric weights are endogenous, calculated from the data itself, and do not require external user input. To validate the index, May values of the index are used within a statistical regression model to model the areal extent of Gulf hypoxia using mid-July cruise measurements from 1985 to 2013, excluding 1989 when no cruise data were available. Regression results (R-adj(2) = 0.81) suggest the index is successful at aggregating multiple pollutants into a single measure of water quality and may be useful for tracking their aggregated effect on the growth of the hypoxia area in the northern Gulf of Mexico. Calculation of the water quality index described here is automatic in the sense that no human intervention is required for variable selection, statistical analysis or assignment of weights. This is very useful for specifying a water quality objective in a multiple objective optimization for watershed management. (C) 2014 Published by Elsevier Ltd. C1 [Whittaker, Gerald; Barnhart, Bradley] ARS, Natl Forage Seed Prod Res Ctr, USDA, Corvallis, OR 97330 USA. [Faere, Rolf; Grosskopf, Shawna] Oregon State Univ, Dept Econ, Corvallis, OR 97331 USA. RP Barnhart, B (reprint author), ARS, Natl Forage Seed Prod Res Ctr, USDA, 3450 Campus Way, Corvallis, OR 97330 USA. EM bradleybarnhart@gmail.com NR 68 TC 2 Z9 2 U1 2 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD FEB PY 2015 VL 49 BP 162 EP 168 DI 10.1016/j.ecolind.2014.10.003 PG 7 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX5GK UT WOS:000346954500019 ER PT J AU Zhang, RM Jang, EB He, SY Chen, JH AF Zhang, Ruimin Jang, Eric B. He, Shiyu Chen, Jiahua TI Lethal and sublethal effects of cyantraniliprole on Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) SO PEST MANAGEMENT SCIENCE LA English DT Article DE oriental fruit fly; cyantraniliprole; sublethal effects; hormesis effect; mating behaviour ID ORIENTAL FRUIT-FLY; ANTHRANILIC DIAMIDE INSECTICIDE; ARMIGERA LEPIDOPTERA-NOCTUIDAE; OLFACTORY-MEDIATED BEHAVIOR; FLIES DIPTERA; HORMESIS; CHLORANTRANILIPROLE; RESISTANCE; TOXICITY; SPINOSAD AB BACKGROUNDThe oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most globally important insect pests. Studies were conducted with the novel anthranilic diamide insecticide cyantraniliprole to determine its lethal and sublethal effects on B. dorsalis. RESULTSAn ingestion toxicity bioassay showed that cyantraniliprole was active against B. dorsalis, and the 72 h feeding LC50 was 3.22 mu g g(-1) in adult diet for a susceptible strain. Sublethal doses of cyantraniliprole (1.30 mu g g(-1) adult diet) induced a hormesis effect on B. dorsalis. The mating competitiveness of B. dorsalis treated with cyantraniliprole at 3.27 mu g g(-1) adult diet was significantly lower when compared with the controls. The lower dose (1.30 mu g g(-1) adult diet) of cyantraniliprole improved the total mating times of both mating pairs in treated groups and also the mating competitiveness of the treated males when compared with the higher dose and controls. Cyantraniliprole-treated females of the mated pairs with the lower dose laid more eggs. On the fifth day, female receptivity in the treated group was significantly reduced when compared with the controls. CONCLUSIONThese results indicate that cyantraniliprole is effective against B. dorsalis. The inhibition and stimulation effect of cyantraniliprole on the adult's mating performance at different concentrations was proved. (c) 2014 Society of Chemical Industry C1 [Zhang, Ruimin; Chen, Jiahua] Fujian Agr & Forestry Univ, Dept Plant Protect, Fuzhou 350002, Fujian, Peoples R China. [Jang, Eric B.] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI USA. [He, Shiyu] Chinese Acad Trop Agr Sci, Guangzhou Expt Stn, Guangzhou, Guangdong, Peoples R China. RP Chen, JH (reprint author), Fujian Agr & Forestry Univ, Dept Plant Protect, Fuzhou 350002, Fujian, Peoples R China. EM Jhchen34@163.com FU Ministry of Agriculture of China FX The authors thank Rui Cardoso Pereira for comments on the manuscript. This study was supported by a grant from the Ministry of Agriculture of China. NR 51 TC 11 Z9 12 U1 1 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2015 VL 71 IS 2 BP 250 EP 256 DI 10.1002/ps.3791 PG 7 WC Agronomy; Entomology SC Agriculture; Entomology GA AX9PN UT WOS:000347234400012 PM 24700426 ER PT J AU Dong, X Zhu, HP Yang, XJ AF Dong, Xiang Zhu, Heping Yang, Xuejun TI Characterization of droplet impact and deposit formation on leaf surfaces SO PEST MANAGEMENT SCIENCE LA English DT Article DE droplet rebound; retention; pesticide spray; adjuvant; high-speed image ID SPRAY RETENTION; EVAPORATION; SYSTEM; ADHESION; LEAVES; AREA AB BACKGROUNDElucidation of droplet dynamic impact and deposit formation on leaf surfaces would assist in modifying application strategies potentially to improve biological control efficiency and minimize pesticide waste. RESULTSThe mechanics of the three-dimensional droplet impact and formation process on hydrophilic and hydrophobic leaf surfaces was investigated with a system that independently controlled droplet diameter, impact speed and impact angle. Spray solutions were made with a nonionic surfactant in distilled water. Water-only droplets rebounded or splashed on hydrophobic leaves of Euphorbia pulcherrima and Zea mays, but not on hydrophilic Dracaena deremensis leaves. Droplet spread areas on D. deremensis at 5.0 m s(-1) impact speed increased 1.9-fold when the droplet diameter was increased from 175 to 481 mu m. Similarly, spread areas of 306 mu m water-only droplets increased 2.0-fold when the impact speed was increased from 3.5 to 7.0 m s(-1). At surfactant concentrations of 0, 0.25, 0.50 and 0.75% (v/v), percentages of droplets that either splashed or bounced off Z. mays were 56, 37, 2 and 0% respectively. CONCLUSIONMicroscopic measurements of droplet impact clarified the effectiveness of surfactants in enhancing droplet retention on hydrophobic leaves and also revealed that water droplets did not rebound or splash on hydrophilic leaves. (c) 2014 Society of Chemical Industry C1 [Dong, Xiang; Yang, Xuejun] Chinese Acad Agr Mechanizat Sci, State Key Lab Soil Plant Machinery Syst Technol, Beijing, Peoples R China. [Zhu, Heping] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, ATRU, Wooster, OH 44691 USA. RP Zhu, HP (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, ATRU, Wooster, OH 44691 USA. EM heping.zhu@ars.usda.gov FU USDA ARS [3607-21620-009-00]; Chinese National Science & Technology Pillar Program [2012BAH29B04] FX The authors gratefully acknowledge B Nudd, A Clark and K Williams of USDA-ARS Application Technology Research Unit for their technical assistance. We are also grateful to USDA ARS (Project No. 3607-21620-009-00) and Chinese National Science & Technology Pillar Program (Grant No. 2012BAH29B04) for the financial support of this research. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture or Chinese Academy of Agricultural Mechanization Sciences. NR 19 TC 5 Z9 6 U1 5 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD FEB PY 2015 VL 71 IS 2 BP 302 EP 308 DI 10.1002/ps.3806 PG 7 WC Agronomy; Entomology SC Agriculture; Entomology GA AX9PN UT WOS:000347234400017 PM 24753323 ER PT J AU Lumpkin, C Penman, JK Rudell, DR Mattheis, JP AF Lumpkin, Christie Penman, John K. Rudell, David R. Mattheis, James P. TI 'Fuji' apple (Malus domestica Borkh.) volatile production during high pCO(2) controlled atmosphere storage SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE CO2 injury; Esters; Fruit quality; Physiological disorders; High pCO(2) stress ID GOLDEN DELICIOUS APPLES; CARBON-DIOXIDE; SUPERFICIAL SCALD; PHYSIOLOGICAL DISORDERS; BROWNING DISORDER; AROMA PRODUCTION; ALPHA-FARNESENE; CO2 INJURY; FRUIT; ETHYLENE AB 'Fuji' apple volatile compound dynamics were characterized during cold storage in air or at low pO(2) controlled atmosphere (CA) with up to 5 kPa CO2. Volatile compounds in storage chambers were adsorbed onto solid sorbent traps and analyzed by GC-MS. Fruit volatile production differed quantitatively by CO2 concentration. Production of butyl, hexyl, and propyl esters by apples stored in CA was lower compared with that by fruit stored in air, while methyl and ethyl ester contents increased during CA storage compared with air. CO2 injury incidence was highest in fruit stored in CA at 5 kPa CO2, an atmosphere where methyl ester accumulation may provide an early indication of CO2 injury development. Published by Elsevier B.V. C1 [Lumpkin, Christie; Penman, John K.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Rudell, David R.; Mattheis, James P.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Mattheis, JP (reprint author), USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM james.mattheis@ars.usda.gov FU Washington Tree Fruit Research Commission FX The authors thank David Buchanan, Janie Countryman and Rachel Leisso for excellent technical assistance. Supplemental funding for this research was received from the Washington Tree Fruit Research Commission. NR 56 TC 3 Z9 4 U1 2 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD FEB PY 2015 VL 100 BP 234 EP 243 DI 10.1016/j.postharvbio.2014.10.008 PG 10 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AY0BN UT WOS:000347263100029 ER PT J AU Bischoff-Ferrari, HA Orav, EJ Kanis, JA Rizzoli, R Schlogl, M Staehelin, HB Willett, WC Dawson-Hughes, B AF Bischoff-Ferrari, H. A. Orav, E. J. Kanis, J. A. Rizzoli, R. Schloegl, M. Staehelin, H. B. Willett, W. C. Dawson-Hughes, B. TI COMPARATIVE PERFORMANCE OF CURRENT DEFINITIONS OF SARCOPENIA AGAINST THE PROSPECTIVE INCIDENCE OF FALLS AMONG COMMUNITY- DWELLING SENIORS AGE 65 AND OLDER SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT WCO-IOF-ESCEO World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases (WCO-IOF-ESCEO) CY MAR 26-29, 2015 CL Milan, ITALY SP WCO, IOF, EsCEO C1 [Bischoff-Ferrari, H. A.] Univ Zurich Hosp, Geriatr & Aging Res, CH-8091 Zurich, Switzerland. [Orav, E. J.] Harvard Univ, Sch Publ Hlth, Biostat, Boston, MA 02115 USA. [Kanis, J. A.] Univ Sheffield, Sheffield, S Yorkshire, England. [Rizzoli, R.] Univ Hosp Geneva, Div Bone Dis, Geneva, Switzerland. [Schloegl, M.] Univ Zurich Hosp, Dept Geriatr & Aging Res, CH-8091 Zurich, Switzerland. [Staehelin, H. B.] Univ Basel, Geriatr, Basel, Switzerland. [Willett, W. C.] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Dawson-Hughes, B.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD FEB PY 2015 VL 26 SU 1 MA OC21 BP S47 EP S48 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DI8QX UT WOS:000373767100030 ER PT J AU Dawson-Hughes, B AF Dawson-Hughes, B. TI EXERCISE, NUTRITION, BONE AND MUSCLES SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT WCO-IOF-ESCEO World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases (WCO-IOF-ESCEO) CY MAR 26-29, 2015 CL Milan, ITALY SP WCO, IOF, EsCEO C1 [Dawson-Hughes, B.] Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD FEB PY 2015 VL 26 SU 1 MA MTE3 BP S66 EP S66 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DI8QX UT WOS:000373767100064 ER PT J AU Ferrari, S Becker, C Lord, SR Studenski, S Warden, SJ Fielding, R Recknor, C Hochberg, MH Blain, H Binder, EF Rolland, Y Poiraudeau, S Benson, CT Myers, SL Hu, L Ahmad, Q Pacuch, K Gomez, E Benichou, O AF Ferrari, S. Becker, C. Lord, S. R. Studenski, S. Warden, S. J. Fielding, R. Recknor, C. Hochberg, M. H. Blain, H. Binder, E. F. Rolland, Y. Poiraudeau, S. Benson, C. T. Myers, S. L. Hu, L. Ahmad, Q. Pacuch, K. Gomez, E. Benichou, O. TI PHASE 2 RANDOMIZED, DOUBLE BLIND, PLACEBO CONTROLLED TRIAL OF MYOSTATIN ANTIBODY IN OLDER FALLERS WITH LOW MUSCLE STRENGTH SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT WCO-IOF-ESCEO World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases (WCO-IOF-ESCEO) CY MAR 26-29, 2015 CL Milan, ITALY SP WCO, IOF, EsCEO C1 [Ferrari, S.] Univ Hosp Geneva, Geneva, Switzerland. [Becker, C.] Robert Bosch Krankenhaus, Stuttgart, Germany. [Lord, S. R.] UNSW, NeuRA, Sydney, NSW, Australia. [Studenski, S.] Univ Pittsburgh, Pittsburgh, PA USA. [Warden, S. J.] Indiana Univ, Sch Hlth & Rehabil Sci, Indianapolis, IN 46204 USA. [Fielding, R.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Recknor, C.] United Osteoporosis Ctr, Gainesville, GA USA. [Hochberg, M. H.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. [Blain, H.] Montpellier Univ Hosp, MacVia LR, Euromov, M2H, Montpellier, France. [Binder, E. F.] Washington Univ, Sch Med, St Louis, MO USA. [Rolland, Y.] Ctr Hosp Univ Toulouse, Gerontopole Toulouse, Toulouse, France. [Rolland, Y.] INSERM 1027, Toulouse, France. [Poiraudeau, S.] Univ Paris 05, Hop Cochin, AP HP,INSERM,U1153, Serv Reeducat & Readaptat Appareil Ocomoteur & Pa, Paris, France. [Benson, C. T.; Myers, S. L.; Hu, L.; Ahmad, Q.; Pacuch, K.; Gomez, E.] Eli Lilly & Co, Indianapolis, IN 46285 USA. [Benichou, O.] Eli Lilly & Co, Neuilly, France. RI POIRAUDEAU, SERGE/P-5301-2016 NR 0 TC 0 Z9 0 U1 2 U2 2 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD FEB PY 2015 VL 26 SU 1 MA OC22 BP S48 EP S48 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DI8QX UT WOS:000373767100031 ER PT J AU Fielding, R AF Fielding, R. TI OSTEOPOROSIS OR SARCOPENIA: TWO DISORDERS OR ONE? SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT WCO-IOF-ESCEO World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases (WCO-IOF-ESCEO) CY MAR 26-29, 2015 CL Milan, ITALY SP WCO, IOF, EsCEO C1 [Fielding, R.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD FEB PY 2015 VL 26 SU 1 MA PL1 BP S29 EP S29 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DI8QX UT WOS:000373767100002 ER PT J AU Reginster, JY Cooper, C Rizzoli, R Kanis, JA Appelboom, G Bautmans, I Bischoff-Ferrari, HA Boers, M Brandi, ML Bruyere, O Cherubini, A Flamion, B Fielding, R Van Loon, L McCloskey, EV Mitlak, B Pilotto, A Reiter-Niesert, S Rolland, Y Tsouderos, Y Visser, M Cruz-Jentoft, AJ AF Reginster, J. -Y. Cooper, C. Rizzoli, R. Kanis, J. A. Appelboom, G. Bautmans, I. Bischoff-Ferrari, H. A. Boers, M. Brandi, M. L. Bruyere, O. Cherubini, A. Flamion, B. Fielding, R. Van Loon, L. McCloskey, E. V. Mitlak, B. Pilotto, A. Reiter-Niesert, S. Rolland, Y. Tsouderos, Y. Visser, M. Cruz-Jentoft, A. J. TI RECOMMENDATIONS FOR THE REGISTRATION OF DRUGS TO TREAT SARCOPENIA SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT WCO-IOF-ESCEO World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases (WCO-IOF-ESCEO) CY MAR 26-29, 2015 CL Milan, ITALY SP WCO, IOF, EsCEO C1 [Reginster, J. -Y.] Univ Liege, Dept Publ Hlth & Hlth Econ, Liege, Belgium. [Cooper, C.] Southampton Gen Hosp, MRC Lifecourse Epidemiol Unit, Southampton SO9 4XY, Hants, England. [Rizzoli, R.] Univ Hosp Geneva, Geneva, Switzerland. [Kanis, J. A.] Univ Sheffield, Sch Med, Sheffield, S Yorkshire, England. [Appelboom, G.] Columbia Univ, Dept Neurol Surg, Med Ctr, New York, NY USA. [Bautmans, I.] Vrije Univ Brussel, Frailty Ageing Res Dept, Brussels, Belgium. [Bischoff-Ferrari, H. A.] Univ Zurich Hosp, Geriatr & Aging Res, CH-8091 Zurich, Switzerland. [Boers, M.] Vrije Univ Amsterdam, Med Ctr, Dept Epidemiol & Biostat, Amsterdam, Netherlands. [Brandi, M. L.] Univ Florence, Florence, Italy. [Cherubini, A.] INRCA Ancona, Dept Gcriatry, Ancona, Italy. [Flamion, B.] Univ Namur, Namur, Belgium. [Fielding, R.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA USA. [Van Loon, L.] Maastricht Univ, Dept Human Movement Sci, Med Ctr, NL-6200 MD Maastricht, Netherlands. [McCloskey, E. V.] Univ Sheffield, Sheffield, S Yorkshire, England. [Mitlak, B.] Eli Lilly & Co, Indianapolis, IN 46285 USA. [Pilotto, A.] Azienda ULSS 16 Padova, Geriatr Unit, Padua, Italy. [Reiter-Niesert, S.] Fed Inst Drugs & Med Devices BfArM, Bonn, Germany. [Rolland, Y.] CHU Toulouse, Clin Gerontol, Toulouse, France. [Tsouderos, Y.] Inst Rech Int Servier, Suresnes, France. [Visser, M.] Vrije Univ Amsterdam, Amsterdam, Netherlands. [Visser, M.] Vrije Univ Amsterdam, Med Ctr, Amsterdam, Netherlands. [Cruz-Jentoft, A. J.] Univ Hosp Ramon & Cajal, Dept Geriatry, Madrid, Spain. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD FEB PY 2015 VL 26 SU 1 MA SE11 BP S62 EP S62 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DI8QX UT WOS:000373767100058 ER PT J AU Boustany, RG Michot, TC Moss, RF AF Boustany, Ronald G. Michot, Thomas C. Moss, Rebecca F. TI Effect of nutrients and salinity pulses on biomass and growth of Vallisneria americana in lower St Johns River, FL, USA SO ROYAL SOCIETY OPEN SCIENCE LA English DT Article DE submerged aquatic vegetation; nutrients; salinity; Vallisneria americana; St Johns River ID WILD CELERY; AQUATIC MACROPHYTES; FLORIDA; DYNAMICS; DECLINE; LIGHT; BAY AB We determined the interactive effects of nutrient loading and salinity pulsing on Vallisneria americana Michx., the dominant submerged aquatic vegetation species in the lower St Johns River (LSJR), FL, USA, and its associated algal community. Five hundred and ninety 6-inch diameter intact plant plugs of Vallisneria were collected from the LSJR in March 2003 and transported to US Geological Survey mesocosm facilities in Lafayette, LA, USA. A 3 x 3 experimental design consisting of three nutrient levels (control, 1/3 control and 3x control) and three salinity pulsing regimes (no pulse, 1-pulse at 18 ppt and 2-pulse at 12 and 18 ppt) was implemented with three replicates per treatment for a total of 27 experimental tanks. Salinity pulsing significantly reduced all measured Vallisneria growth parameters including above-and below-ground biomass, areal productivity and leaf area index. Nutrient levels had little effect on plants subjected to salinity pulses, but in non-salinity pulse treatments we observed higher mean macrophyte biomass in the low-nutrient loading treatments. Macroalgal components (epiphytes and surface algal mats) were not significantly different (p = 0.2998 and p = 0.2444, respectively), but water column chlorophyll a (phytoplankton) was significantly higher (p < 0.0001) in all salinity pulse treatments except for the 1-pulse, low-nutrient treatment. A single salinity pulse at 18 ppt resulted in 22% pot mortality and two consecutive pulses of 18 and 12 ppt resulted in an additional 14% mortality. Individual leaves and ramets lost 59.7% and 67.8%, respectively, in the combined salinity pulse treatments. Nutrient loading tends to have a long-term effect on Vallisneria through complex community interactions while salinity pulsing frequency and intensity has an immediate and direct influence on growth and distribution. C1 [Boustany, Ronald G.; Michot, Thomas C.] US Geol Survey, Lafayette, LA 70506 USA. [Moss, Rebecca F.] US Geol Survey, Five Rivers Serv LLC, Natl Wetlands Res Ctr, Lafayette, LA 70506 USA. [Michot, Thomas C.] Univ Louisiana Lafayette, Inst Coastal Ecol & Engn, Lafayette, LA 70504 USA. RP Boustany, RG (reprint author), USDA, Nat Resources Conservat Serv, 646 Cajundome Blvd,Suite 180, Lafayette, LA 70506 USA. EM ron.boustany@la.usda.gov FU St Johns River Water Management District (State of Florida); US Geological Survey National Wetlands Research Center FX This project was funded by the St Johns River Water Management District (State of Florida) and the US Geological Survey National Wetlands Research Center. NR 37 TC 0 Z9 0 U1 5 U2 6 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 2054-5703 J9 ROY SOC OPEN SCI JI R. Soc. Open Sci. PD FEB PY 2015 VL 2 IS 2 AR 140053 DI 10.1098/rsos.140053 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO7LS UT WOS:000377964700001 PM 26064592 ER PT J AU Chiou, BS Valenzuela-Medina, D Bilbao-Sainz, C Klamczynski, AK Avena-Bustillos, RJ Milczarek, RR Du, WX Glenn, GM Orts, WJ AF Chiou, Bor-Sen Valenzuela-Medina, Diana Bilbao-Sainz, Cristina Klamczynski, Artur K. Avena-Bustillos, Roberto J. Milczarek, Rebecca R. Du, Wen-Xian Glenn, Greg M. Orts, William J. TI Torrefaction of pomaces and nut shells SO BIORESOURCE TECHNOLOGY LA English DT Article DE Torrefaction; Pomaces; Nut shells; Response surface methodology; Mass and energy yields ID OIL PALM BIOMASS; HYDROTHERMAL CARBONIZATION; SYNTHETIC INDICATOR; MASS-LOSS; WOOD; IMPACT; FUEL; TRANSFORMATION; GRINDABILITY; GASIFICATION AB Apple, grape, olive, and tomato pomaces as well as almond and walnut shells were torrefied at different temperatures and times in a muffle furnace. The fiber content and thermal stability of the raw byproducts were examined and the moisture and ash contents, elemental composition, and gross calorific values of the raw and torrefied samples were characterized. Response surface methodology and a central composite design were used to examine the effects of temperature and time on mass and energy yields of the torrefied byproducts. Raw apple pomace had the highest hemicellulose content, whereas raw grape pomace had the highest lignin content. Raw tomato pomace had the highest gross calorific value because of its high carbon content. Temperature had a larger effect on mass and energy yields than time. Grape pomace generally had the highest mass and energy yields. Also, energy yields of the byproducts could be predicted from mass loss values. Published by Elsevier Ltd. C1 [Chiou, Bor-Sen; Valenzuela-Medina, Diana; Bilbao-Sainz, Cristina; Klamczynski, Artur K.; Avena-Bustillos, Roberto J.; Milczarek, Rebecca R.; Du, Wen-Xian; Glenn, Greg M.; Orts, William J.] USDA, Albany, CA 94710 USA. RP Chiou, BS (reprint author), USDA, Albany, CA 94710 USA. EM bor-sen.chiou@ars.usda.gov FU California Department of Food and Agriculture's Specialty Crop Block Grant Program [SCB11021] FX This study was funded by the California Department of Food and Agriculture's Specialty Crop Block Grant Program (#SCB11021). NR 38 TC 5 Z9 5 U1 3 U2 53 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD FEB PY 2015 VL 177 BP 58 EP 65 DI 10.1016/j.biortech.2014.11.071 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AX0MT UT WOS:000346647100008 PM 25479394 ER PT J AU Pradeep, S Josh, MKS Binod, P Devi, RS Balachandran, S Anderson, RC Benjamin, S AF Pradeep, S. Josh, M. K. Sarath Binod, P. Devi, R. Sudha Balachandran, S. Anderson, Robin C. Benjamin, Sailas TI Achromobacter denitrificans strain SP1 efficiently remediates di(2-ethylhexyl)phthalate SO ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY LA English DT Article DE Achromobacter denitrificans SP1; Biodegradation; Response surface methodology; Blood storage bag; DEHP; PVC ID RESPONSE-SURFACE METHODOLOGY; POLYVINYL-CHLORIDE; DI-2-ETHYLHEXYL PHTHALATE; MICROBIAL-DEGRADATION; DIMETHYL PHTHALATE; BIODEGRADATION; ESTERS; DEHP; PLASTICIZER; METABOLITES AB This study describes how Achromobacter denitrificans strain SP1, a novel isolate from heavily plastics-contaminated sewage sludge efficiently consumed the hazardous plasticizer, di(2-ethylhexyl)phthalate (DEHP) as carbon source supplemented in a simple basal salt medium (BSM). Response surface methodology was employed for the statistical optimization of the process parameters such as temperature (32 C), agitation (200 rpm), DEHP concentration (10 mM), time (72 h) and pH (8.0). At these optimized conditions, experimentally observed DEHP degradation was 63%, while the predicted value was 59.2%; and the correlation coefficient between them was 0.998, i.e., highly significant and fit to the predicted model. Employing GC-MS analysis, the degradation pathway was partially deduced with intermediates such as mono(2-ethylhexyl)phthalate and 2-ethyl hexanol. Briefly, this first report describes A. denitrificans strain SP1 as a highly efficient bacterium for completely remediating the hazardous DEHP (10 mM) in 96 h in BSM (50% consumed in 60 h), which offers great potentials for efficiently cleaning the DEHP-contaminated environments such as soil, sediments and water upon its deployment. (C) 2014 Elsevier Inc. All rights reserved. C1 [Pradeep, S.; Josh, M. K. Sarath; Benjamin, Sailas] Univ Calicut, Dept Bot, Div Biotechnol, Enzyme Technol Lab, Malappuram 673635, Kerala, India. [Binod, P.] CSIR, Natl Inst Interdisciplinary Sci & Technol, Thiruvananthapuram 695019, Kerala, India. [Devi, R. Sudha; Balachandran, S.] Univ Kerala, Mahatma Gandhi Coll, Dept Chem, Thiruvananthapuram 695004, Kerala, India. [Anderson, Robin C.] USDA ARS, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Benjamin, S (reprint author), Univ Calicut, Dept Bot, Div Biotechnol, Enzyme Technol Lab, Malappuram 673635, Kerala, India. EM benjamin@uoc.ac.in RI MOOLAKKARIYIL, SARATH JOSH/C-5596-2015; TVM, NIIST/E-5132-2012; S, Pradeep/C-5614-2015 OI MOOLAKKARIYIL, SARATH JOSH/0000-0002-9624-8558; TVM, NIIST/0000-0002-5814-466X; FU Ministry of Environment and Forests, Government of India for a research [19/62/2005-RE]; Department of Biotechnology, Government of India for a research [BT/PR7521/BCE/8/1026/2013] FX The authors would like to thank the Ministry of Environment and Forests, Government of India for a research Grant no. 19/62/2005-RE and Department of Biotechnology, Government of India for a research Grant no. BT/PR7521/BCE/8/1026/2013. The authors also declare that there exists no competing conflict of interests. NR 41 TC 10 Z9 12 U1 1 U2 23 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0147-6513 EI 1090-2414 J9 ECOTOX ENVIRON SAFE JI Ecotox. Environ. Safe. PD FEB PY 2015 VL 112 BP 114 EP 121 DI 10.1016/j.ecoenv.2014.10.035 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA AX2EC UT WOS:000346755400016 PM 25463861 ER PT J AU Bolton, SJ Bauchan, GR Ochoa, R Pooley, C Klompen, H AF Bolton, Samuel J. Bauchan, Gary R. Ochoa, Ronald Pooley, Christopher Klompen, Hans TI The role of the integument with respect to different modes of locomotion in the Nematalycidae (Endeostigmata) SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Peristalsis; Striations; Anal valves; LT-SEM; Videography ID ACARI; LIFE AB Previous research on the locomotion of the Nematalycidae has only been undertaken on Gordialycus, which is by far the most elongated genus of the family. Gordialycus is dependent on an unusual form of peristalsis to move around. It was not known whether the genera of Nematalycidae with shorter bodies also used this mode of locomotion. Our videographic recordings of Osperalycus did not reveal peristalsis. Instead, this mite appears to move around the mineral regolith via the expansion and constriction of the metapodosomal and genital region, allowing greater versatility in the way that the annular regions contract and extend. This type of locomotion would enable relatively short bodied nematalycids to anchor themselves into secure positions before extending their anterior regions through tight spaces. Low-temperature scanning electron micrographs show that the short bodied genera have integumental features that appear to be associated with this mode of locomotion. Peristalsis is almost certainly a more derived form of locomotion that is an adaptation to the unusually long body form of Gordialycus. C1 [Bolton, Samuel J.; Klompen, Hans] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Acarol Lab, Columbus, OH 43212 USA. [Bauchan, Gary R.; Pooley, Christopher] ARS, Electron & Confocal Microscopy Unit, USDA, BARC West, Beltsville, MD 20705 USA. [Ochoa, Ronald] ARS, Systemat Entomol Lab, USDA, BARC West, Beltsville, MD 20705 USA. RP Bolton, SJ (reprint author), Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Acarol Lab, 1315 Kinnear Rd, Columbus, OH 43212 USA. EM samuel.bolton77@googlemail.com FU Smithsonian Institution FX This research was partly funded by the Smithsonian Institution (pre-doctoral fellowship to SJB). Thanks to Elizabeth Murray and John Heraty for the use of their lab facilities at the University of California, Riverside. An acknowledgment is also due to the US. National Park Service for granting the first author permission to collect from the Indiana Dunes National Lakeshore. Mention of trade names or commercial products in this publication does not imply recommendation or endorsement by the USDA or The Ohio State University. Figures 1, 2, 3, 4, 5 are sourced from the US Department of Agriculture, Agricultural Research Service, Electron and Confocal Microscopy Unit, Beltsville, Maryland, USA. These images are in the public domain. NR 11 TC 2 Z9 2 U1 1 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD FEB PY 2015 VL 65 IS 2 BP 149 EP 161 DI 10.1007/s10493-014-9857-0 PG 13 WC Entomology SC Entomology GA AX2LP UT WOS:000346775700001 PM 25355075 ER PT J AU Phillips, J Marion, DA Yocum, C Mehlhope, SH Olson, JW AF Phillips, Jonathan Marion, Daniel A. Yocum, Chad Mehlhope, Stephanie H. Olson, Jeff W. TI Geomorphological impacts of a tornado disturbance in a subtropical forest SO CATENA LA English DT Article DE Geomorphic disturbance; Bioturbation; Tornado blowdown; Uprooting; Biogeomorphology; Ouachita Mountains ID SPATIAL SCALES; SOIL FORMATION; ENERGY; TREETHROW; VARIABILITY; VEGETATION; WINDTHROW; MOUNTAINS; EVOLUTION; REGOLITH AB We studied tree uprooting associated with an EF2 tornado that touched down in portions of the Ouachita Mountains in Western Arkansas in 2009. In the severe blowdown areas all trees in the mixed shortleaf pine-hardwood forest were uprooted or broken, with no relationship between tree species or size and whether uprooting or breakage occurred. There was also no significant relationship between tree species and amount of soil displaced, and only a weak relationship between tree size and rootwad size. Uprooting resulted in a mean bioturbation rate of 205 m(3) ha(-1) (about 240 t ha(-1)). Direct transfer of wind energy via tree uprooting to geomorphic work of soil displacement was about 75 to 190J m(-2). Given the infrequency of tornadoes, this energy subsidy is minor with respect to the long-term energetics of pedogenesis and landscape evolution. However, it does represent a highly significant pulse of geomorphically-significant energy relative to other mechanical processes. Tornadoes such as that of April, 2009-not atypical for the region-are disturbances causing severe, non-selective impacts withinthe affected area. At a broader, landscape scale, tornadoes are highly localized disturbances, and occur infrequently within any given landform element or forest stand. Only about a third of the uproots revealed root penetration of bedrock, compared to about 90% in other areas of the Ouachita Mountains. This is attributable to the thicker colluvial soils at the study site, and is consistent with the idea that root-bedrock interaction is more likely in thinner regolith covers. (C) 2014 Elsevier B.V. All rights reserved. C1 [Phillips, Jonathan; Marion, Daniel A.] Univ Kentucky, Dept Geog, Lexington, KY 40506 USA. [Marion, Daniel A.; Yocum, Chad] USDA, Forest Serv, Southern Res Stn, Hot Springs, AR 71902 USA. [Mehlhope, Stephanie H.] Univ Kentucky, Dept Agr & Biosyst Engn, Lexington, KY 40506 USA. [Olson, Jeff W.] USDA, Forest Serv, Hot Springs, AR 71902 USA. RP Phillips, J (reprint author), Univ Kentucky, Dept Geog, Lexington, KY 40506 USA. EM jdp@uky.edu OI Marion, Daniel/0000-0002-5871-8969 FU U.S. Department of Agriculture Forest Service; Ouachita National Forest; Southern Research Station FX Paul Bradway and Jidan Duan assisted in field data collection. The U.S. Department of Agriculture Forest Service, Southern Research Station, and the Ouachita National Forest provided financial and logistic support. NR 56 TC 6 Z9 6 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD FEB PY 2015 VL 125 BP 111 EP 119 DI 10.1016/j.catena.2014.10.014 PG 9 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA AW6SL UT WOS:000346398600011 ER PT J AU Paul, M Baranzoni, GM Albonetti, S Brewster, JD AF Paul, Moushumi Baranzoni, Gian Marco Albonetti, Sabrina Brewster, Jeffrey D. TI Direct, quantitative detection of Listeria monocytogenes in fresh raw whole milk by qPCR SO INTERNATIONAL DAIRY JOURNAL LA English DT Article ID REAL-TIME PCR; FOOD; PRODUCTS; DNA AB A method previously developed for direct (non-enrichment) detection of Escherichia coli O157:H7 was adapted for Listeria monocytogenes. The sample treatment protocol was successful in concentrating bacteria from 10 mL raw milk samples and reducing PCR inhibition, but qPCR detection sensitivity and reproducibility was poor. Two DNA extraction reagents and multiple extraction conditions were tested to identify an efficient and reproducible DNA extraction method. Two primer/probe sets were evaluated at two concentrations and three annealing temperatures to minimize false-positive results and optimize sensitivity and reproducibility of qPCR detection. Under the selected conditions, DNA was extracted efficiently from the entire milk sample in a volume of 10 mL, and subsequently quantitated by a 5' nuclease qPCR assay lasting 50 min. The method provided detection of 1 cfu mL(-1) L. monocytogenes in 10 mL raw milk and quantitation from 10 to 1000 cfu mL(-1) with a total time to result of <3 h. Published by Elsevier Ltd. C1 [Paul, Moushumi] USDA ARS, Dairy & Funct Foods Res Unit, NAA, ERRC, Wyndmoor, PA 19038 USA. [Brewster, Jeffrey D.] USDA ARS, Mol Characterizat Foodborne Pathogens Res Unit, NAA, ERRC, Wyndmoor, PA 19038 USA. [Baranzoni, Gian Marco; Albonetti, Sabrina] Univ Bologna, Dept Vet Med Sci, I-40064 Ozzano Dell Emilia, Italy. RP Brewster, JD (reprint author), USDA ARS, Mol Characterizat Foodborne Pathogens Res Unit, NAA, ERRC, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM jeffrey.brewster@ars.usda.gov NR 13 TC 4 Z9 4 U1 2 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0958-6946 EI 1879-0143 J9 INT DAIRY J JI Int. Dairy J. PD FEB PY 2015 VL 41 BP 46 EP 49 DI 10.1016/j.idairyj.2014.09.008 PG 4 WC Food Science & Technology SC Food Science & Technology GA AU3JC UT WOS:000345507700009 ER PT J AU Jin, TZ Guo, MM Zhang, HQ AF Jin, Tony Z. Guo, Mingming Zhang, Howard Q. TI Upscaling from benchtop processing to industrial scale production: More factors to be considered for pulsed electric field food processing SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE PEF; Juice; Scale-up; Commercialization; Critical parameters; Processing conditions ID ESCHERICHIA-COLI; ORANGE JUICE; LACTOBACILLUS-PLANTARUM; PROCESS PARAMETERS; SACCHAROMYCES-CEREVISIAE; INACTIVATION KINETICS; COST-ANALYSIS; APPLE JUICE; SHELF-LIFE; PASTEURIZATION AB Pulsed electric field (PEF) processing of juice has been intensively studied with benchtop scale experiments. However, there is still limited information regarding critical factors to be considered for PEF efficacy in microbial reduction with PEF processing during pilot or commercial scale production of juice. In the present study, continuous benchtop (3.6-7.2 L/h) PEF processing systems with co-field treatment chambers and bipolar square waveform pulses were used and simulated production conditions were tested for pomegranate juice. Microbial reductions of Escherichia coli, as affected by PEF process conditions (field strength, pulse width, pulse frequency, total treatment time, input energy), production conditions (flow rate, juice holding time and temperature), and juice properties (pH, conductivity, particulate), were investigated. Flow rate, PEF process parameters, production conditions, type of target microorganism, and properties of juice significantly affected microbial reductions by PEF treatments. E. coli ATCC 35218, a non pathogenic surrogate bacterium, exhibited higher resistance to PEF treatments than E. coli 0157:H7 and E. coli K12 in pomegranate juice. Increase of a single PEF parameter (field strength, pulse width, pulse frequency, total treatment time, or energy input) is insufficient to achieve maximum microbial reduction. Optimal PEP treatment conditions for maximum microbial reduction depend on multiple factors including PEF processing parameters, production conditions and product properties. This study demonstrates that scale-up and validation studies in a specific PEF system for specific products are very important and necessary before successful commercial application of this novel technology is possible. Published by Elsevier Ltd. C1 [Jin, Tony Z.; Guo, Mingming] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Guo, Mingming] Jiangnan Univ, Wuxi 214122, Jiangsu, Peoples R China. [Zhang, Howard Q.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Jin, TZ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ars.usda.gov OI Jin, Tony/0000-0003-0504-5817 NR 57 TC 5 Z9 5 U1 4 U2 38 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD FEB PY 2015 VL 146 BP 72 EP 80 DI 10.1016/j.jfoodeng.2014.08.020 PG 9 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA AT8LH UT WOS:000345184300010 ER PT J AU Nyamadzawo, G Wuta, M Nyamangara, J Rees, RM Smith, JL AF Nyamadzawo, George Wuta, Menas Nyamangara, Justice Rees, Robert M. Smith, Jeffry L. TI The effects of catena positions on greenhouse gas emissions along a seasonal wetland (dambo) transect in tropical Zimbabwe SO ARCHIVES OF AGRONOMY AND SOIL SCIENCE LA English DT Article DE carbon dioxide; methane; seasonal wetland (dambo); dambo emissions; catena; nitrous oxide ID AMAZON RIVER FLOODPLAIN; METHANE EMISSIONS; WATER-RESOURCES; NITROUS-OXIDE; RICE FIELDS; SOILS; CARBON; FLUX; OXIDATION; SAVANNA AB Wetlands are major natural sources of greenhouse gases (GHGs). In central and southern Africa, one of the most extensive wetlands are dambos (seasonal wetlands) which occupy 20-25% of land area. However, there are very little data on GHG methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O) emissions from dambos, and this study presents the first estimates from dambos in Zimbabwe. The objective was to evaluate the effects of catena positions; upland, dambo mid-slope and dambo bottom, on GHG emissions along an undisturbed dambo transect. Methane emissions were -0.3, 29.5 and -1.3mgm(-2)hr(-1), N2O emission were 40.1, 3.9 and 5.5 mu gm(2)hr(-1), while CO2 emissions were 2648.9, 896.2 and 590.1mgm(-2)hr(-1) for upland, mid-slope and bottom catena, respectively. Our results showed that uplands were important sources of N2O and CO2, and a sink for CH4, while the dambo mid-slope position was a major source of CH4, but a weak source of CO2 and N2O. Dambo bottom catena was weak source GHGs. Overall, dambos were major sources of CH4 and weak sources of N2O and CO2.We concluded that, depending on catenal position, dambos can be major or minor sources of GHGs. C1 [Nyamadzawo, George; Wuta, Menas] Univ Zimbabwe, Dept Soil Sci & Agr Engn, Harare, Zimbabwe. [Nyamadzawo, George] Bindura Univ Sci Educ, Dept Environm Sci, Bindura, Zimbabwe. [Nyamangara, Justice] Int Crops Res Inst Semi Arid Trop, Matopos Res Stn, Bulawayo, Zimbabwe. [Rees, Robert M.] Scotlands Rural Coll, Div Agron, Edinburgh, Midlothian, Scotland. [Smith, Jeffry L.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Nyamadzawo, G (reprint author), Univ Zimbabwe, Dept Soil Sci & Agr Engn, Harare, Zimbabwe. EM gnyama@yahoo.com RI Rees, Robert/E-8333-2010; OI Rees, Robert/0000-0003-1348-8693 FU International Foundation of Science (IFS) [C/4569-1]; DAAD Fellowship [A/10/03022]; CLIFF network under the Research program on Climate change Agriculture and Food Security (CCAFS) FX We are grateful for the funding from the International Foundation of Science (IFS) [grant number C/4569-1]; DAAD Fellowship [grant number A/10/03022] and CLIFF network under the Research program on Climate change Agriculture and Food Security (CCAFS). NR 60 TC 0 Z9 0 U1 1 U2 50 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0365-0340 EI 1476-3567 J9 ARCH AGRON SOIL SCI JI Arch. Agron. Soil Sci. PD FEB 1 PY 2015 VL 61 IS 2 BP 203 EP 221 DI 10.1080/03650340.2014.926332 PG 19 WC Agronomy; Soil Science SC Agriculture GA AS6LY UT WOS:000344376200005 ER PT J AU Boyette, CD Hoagland, RE AF Boyette, Clyde Douglas Hoagland, Robert E. TI Bioherbicidal potential of Xanthomonas campestris for controlling Conyza canadensis SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE bioherbicide; biological control; horseweed; Xanthomonas campestris; Conyza canadensis ID BROME BROMUS-TECTORUM; BIOLOGICAL-CONTROL; SESBANIA-EXALTATA; INVERT EMULSION; TILLAGE SYSTEMS; WEED MANAGEMENT; POAE JT-P482; GLYCINE-MAX; HORSEWEED; GLYPHOSATE AB The effects of environmental parameters on bioherbicidal activity of the bacterium Xanthomonas campestris, against glyphosate-resistant and - susceptible Conyza canadensis (horseweed), were studied under greenhouse conditions. Rosette leaf-stage plants were more susceptible than older plants, and increasing inoculum from 10(5) to 10(9) cells/mL caused significantly greater plant mortality and biomass reduction of plants in both the rosette and bolting growth stages. A dew period at 25 degrees C was required to cause an 80% and 60% mortality of plants in the rosette and bolting growth stages, respectively. Results indicate that X. campestris can infect and kill horseweed, demonstrating its bioherbicidal potential. C1 [Boyette, Clyde Douglas] ARS, Biol Control Pests Res Unit, USDA, Stoneville, MS 38776 USA. [Hoagland, Robert E.] ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS USA. RP Boyette, CD (reprint author), ARS, Biol Control Pests Res Unit, USDA, Stoneville, MS 38776 USA. EM doug.boyette@ars.usda.gov NR 32 TC 3 Z9 3 U1 2 U2 22 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD FEB 1 PY 2015 VL 25 IS 2 BP 229 EP 237 DI 10.1080/09583157.2014.966650 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AS1PM UT WOS:000344052600010 ER PT J AU McKay, DL Chen, CYO Zampariello, CA Blumberg, JB AF McKay, Diane L. Chen, C. -Y. Oliver Zampariello, Carly A. Blumberg, Jeffrey B. TI Flavonoids and phenolic acids from cranberry juice are bioavailable and bioactive in healthy older adults SO FOOD CHEMISTRY LA English DT Article DE Cranberry; Polyphenols; Flavonoids; Phenolics; Proanthocyanidins; Antioxidant; Bioavailability ID PLASMA ANTIOXIDANT CAPACITY; DOUBLE-BLIND; IN-VITRO; DISEASE RISK; CONSUMPTION; ANTHOCYANINS; METABOLISM; HUMANS; BLUEBERRY; CANCER AB Cranberries (Vaccinium macrocarpon) are a rich source of phenolic phytochemicals, which likely contribute to their putative health benefits. A single-dose pharmacokinetic trial was conducted in 10 healthy adults >= 50 y to evaluate the acute (24-h) absorption and excretion of flavonoids, phenolic acids and proanthocyanidins (PACs) from a low-calorie cranberry juice cocktail (54% juice). Inter-individual variability was observed in the C-max and T-max of many of these compounds in both plasma and urine. The sum total concentration of phenolics detected in plasma reached a peak of 34.2 mu g/ml between 8 and 10 h, while in urine this peak was 269.8 mu g/mg creatinine, and appeared 2-4 h earlier. The presence of PAC-A2 dimers in human urine has not previously been reported. After cranberry juice consumption, plasma total antioxidant capacity assessed using ORAC and TAP assays correlated with individual metabolites. Our results show phenolic compounds in cranberry juice are bioavailable and exert antioxidant actions in healthy older adults. (C) 2014 Elsevier Ltd. All rights reserved. C1 [McKay, Diane L.; Chen, C. -Y. Oliver; Zampariello, Carly A.; Blumberg, Jeffrey B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. RP McKay, DL (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, 711 Washington St, Boston, MA 02111 USA. EM diane.mckay@tufts.edu FU USDA Agricultural Research Service [58-1950-014]; Ocean Spray; U.S. Department of Agriculture [58-1950-0-014] FX Supported by the USDA Agricultural Research Service under Cooperative Agreement No. 58-1950-014 and Ocean Spray. This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 42 TC 34 Z9 34 U1 11 U2 279 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD FEB 1 PY 2015 VL 168 BP 233 EP 240 DI 10.1016/j.foodchem.2014.07.062 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AR5GJ UT WOS:000343612800031 PM 25172705 ER PT J AU Treuhaft, R Goncalves, F dos Santos, JR Keller, M Palace, M Madsen, SN Sullivan, F Graca, PMLA AF Treuhaft, Robert Goncalves, Fabio dos Santos, Joao Roberto Keller, Michael Palace, Michael Madsen, Soren N. Sullivan, Franklin Graca, Paulo M. L. A. TI Tropical-Forest Biomass Estimation at X-Band From the Spaceborne TanDEM-X Interferometer SO IEEE GEOSCIENCE AND REMOTE SENSING LETTERS LA English DT Article DE Biomass; interferometric synthetic aperture radar (InSAR); lidar; tropical forest ID SAR INTERFEROMETRY; CARBON; LIDAR; BALANCE; RADAR AB This letter reports the sensitivity of X-band interferometric synthetic aperture radar (InSAR) data from the first dual-spacecraft radar interferometer, TanDEM-X, to variations in tropical-forest aboveground biomass (AGB). It also reports the first tropical-forest AGB estimates from TanDEM-X data. Tropical forests account for about 50% of the world's forested biomass and play critical roles in the control of atmospheric carbon dioxide by emission through deforestation and uptake through forest growth. The TanDEM-X InSAR data used in this analysis were taken over the Tapajos National Forest, Par, Brazil, where field measurements from 30 stands were acquired. The magnitude of the InSAR normalized complex correlation, which is called coherence, decreases by about 25% as AGB increases from 2 to 430 Mg-ha(-1), suggesting more vertically distributed return-power profiles with increasing biomass. Comparison of InSAR coherences to those of small-spot (15 cm) lidar suggests that lidar penetrates deeper into the canopies than InSAR. Modeling InSAR profiles from InSAR coherence and lidar profiles yields an estimate of 0.29 dB/m for the X-band extinction coefficient relative to that of lidar. Forest AGB estimated from InSAR observations on 0.25-ha plots shows RMS scatters about the field-estimated AGB between 52 and 62 Mg-ha(-1), which is between 29% and 35% of the average AGB of 179 Mg-ha(-1), depending on the data analysis mode. The sensitivity and biomass-estimation performance suggest the potential of TanDEM-X observations to contribute to global tropical-forest biomass monitoring. C1 [Treuhaft, Robert; Madsen, Soren N.] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. [Goncalves, Fabio] Woods Hole Res Ctr, Falmouth, MA 02540 USA. [dos Santos, Joao Roberto] Inst Nacl Pesquisas Espaciais, BR-12227010 Sao Jose Dos Campos, Brazil. [Keller, Michael] USDA, Forest Serv, Int Inst Trop Forestry, San Juan, PR 00926 USA. [Keller, Michael] Empresa Brasileira Pesquisa Agropecuaria EM BRAPA, BR-13070115 Campinas, SP, Brazil. [Keller, Michael; Palace, Michael; Sullivan, Franklin] Univ New Hampshire, Durham, NH 03824 USA. [Graca, Paulo M. L. A.] Inst Nacl de Pesquisas da Amazonia, BR-69067375 Manaus, AM, Brazil. RP Treuhaft, R (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. EM robert.treuhaft@jpl.nasa.gov; fgoncalves@whrc.org; jroberto@dsr.inpe.br; mkeller.co2@gmail.com; palace@guero.sr.unh.edu; soren.n.madsen@jpl.nasa.gov; fsulliva@gmail.com; pmlag@inpa.gov.br RI de Alencastro Graca, Paulo Mauricio/B-3375-2013; Keller, Michael/A-8976-2012 OI Keller, Michael/0000-0002-0253-3359 NR 25 TC 14 Z9 14 U1 3 U2 101 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1545-598X EI 1558-0571 J9 IEEE GEOSCI REMOTE S JI IEEE Geosci. Remote Sens. Lett. PD FEB PY 2015 VL 12 IS 2 BP 239 EP 243 DI 10.1109/LGRS.2014.2334140 PG 5 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA AO7YA UT WOS:000341567600005 ER PT J AU Williams, MR King, KW Fausey, NR AF Williams, M. R. King, K. W. Fausey, N. R. TI Drainage water management effects on tile discharge and water quality SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Controlled drainage; Nitrogen; Phosphorus; Before-after control-impact (BACI); Best management practice (BMP) ID NITRATE LOSS; AGRICULTURAL DRAINAGE; IMPACTS; LOADS; SOIL; SUBIRRIGATION; TEMPERATURE; TILLAGE; SYSTEMS; LOSSES AB Drainage water management (DWM) has received considerable attention as a potential best management practice (BMP) for improving water quality in tile drained landscapes. The objective of this study was to evaluate the effects of DWM on subsurface drain discharge as well as on nitrogen (N) and phosphorus (P) loads in drainage water. Tile discharge and nutrient concentrations were measured from two adjacent tile drainage outlets in an Ohio, USA headwater watershed for 7 years (2006-2012). A control structure was installed in 2009 to allow DWM at one of the outlets from 2009 to 2012. A before-after control-impact (BACI) study design was used to assess the impact of DWM on tile discharge and nutrient loads. Results showed that DWM significantly decreased annual tile discharge between 11 and 178 mm, which was equivalent to an 8 to 34% reduction in flow. DWM significantly decreased annual NO3-N loads by -1.3 to 26.8 kg ha(-1) (-8 to 44%) and annual dissolved P loads by 0.04 to 0.51 kg ha(-1) (40 to 68%). Nutrient concentrations were not significantly affected by DWM indicating that decreases in nutrient loads were primarily due to reductions in tile discharge rather than changes in concentration. Results from the current study support the use of DWM as a BMP to decrease N and P loads in subsurface drain discharge throughout the U.S. Midwest. Future research should focus on quantifying the effect of DWM on nutrient transport in other flow paths (e.g., lateral seepage, surface runoff) to further evaluate its use as a BMP in tile drained landscapes. Published by Elsevier B.V. C1 [Williams, M. R.; King, K. W.; Fausey, N. R.] USDA ARS, Soil Drainage Res Unit, Columbus, OH 43210 USA. RP Williams, MR (reprint author), USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM mark.williams2@ars.usda.gov; kevin.king@ars.usda.gov; norm.fausey@ars.usda.gov NR 39 TC 12 Z9 12 U1 4 U2 57 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JAN 31 PY 2015 VL 148 BP 43 EP 51 DI 10.1016/j.agwat.2014.09.017 PG 9 WC Agronomy; Water Resources SC Agriculture; Water Resources GA AW8XA UT WOS:000346541500006 ER PT J AU Jenkins, MB Adams, MP Endale, DM Fisher, DS Lowrance, R Newton, GL Vellidis, G AF Jenkins, Michael B. Adams, M. Paige Endale, Dinku M. Fisher, Dwight S. Lowrance, Richard Newton, G. Larry Vellidis, George TI Storm flow dynamics and loads of fecal bacteria associated with ponds in southern piedmont and coastal plain watersheds with animal agriculture SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE E. coli 0157:H7; Fecal indicator bacteria; Ponds; Salmonella; Storm flow; Watersheds ID QUANTIFYING DILUTE CONCENTRATIONS; PROBABLE NUMBER METHODOLOGY; ESCHERICHIA-COLI; SURFACE WATERS; TRANSPORT; SURVIVAL; MICROORGANISMS; IMPACT; FLUXES; FATE AB Storm events that increase flow rates can disturb sediments and produce overland runoff in watersheds with animal agriculture, and, thus, can increase surface water concentrations of fecal bacteria and risk to public health. We tested the hypothesis that strategically designed and placed ponds in watersheds with animal agriculture would attenuate downstream fluxes of fecal bacteria. We measured concentrations and fluxes of fecal indicator bacteria (commensal Escherichia coli and fecal enterococci) and manure pathogens (Salmonella and E. coli 0157:H7) in in- and outflows of Bishop Pond in the Southern Piedmont of Georgia during three storm events and in- and outflow concentrations and fluxes of fecal indicator bacteria at Ponds A and C in the Coastal Plain of Georgia during two storm events. Mean concentrations and fluxes of fecal indicator bacteria associated with pond in- and outflow during hydrograph rise, peak, fall, and 5-days after peak flow at Bishop Pond were significantly greater than their mean base flow concentrations and fluxes. In storm flow Bishop Pond significantly reduced the outflow concentrations and fluxes of fecal indicator bacteria compared with corresponding inflow measurements. Unlike fecal indicator bacteria, Bishop Pond appeared not to reduce outflow concentrations and fluxes of Salmonella or E. coli 0157:H7. At Ponds A and C in the Coastal Plain mean in- and outflow concentrations and fluxes of the fecal indicator bacteria associated with the hydrograph rise and peak flows of the storms were not different Bishop Pond, with a length to width ratio of 3.3, attenuated downstream fluxes of fecal bacteria. In contrast, Ponds A and C were not effective at reducing downstream fluxes of fecal bacteria under storm flow conditions. The ineffectiveness of Ponds A and C may be attributed to their having length to width ratios of 1.2 and 2.5, respectively, both of which are below the minimum for effective pond performance. Our results indicated that in the humid Southeast an appropriately placed and configured pond in watersheds with animal agriculture can reduce storm flow loads of fecal indicator bacteria but not necessarily pathogenic E coli 0157:H7. Published by Elsevier B.V. C1 [Jenkins, Michael B.] USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Unit, Oxford, MS 38655 USA. [Adams, M. Paige] Univ Georgia, Dept Biol & Agr Engn, Tifton, GA 31793 USA. [Endale, Dinku M.; Lowrance, Richard] USDA ARS, Southeast Watershed Res Lab, Tifton, GA 31793 USA. [Fisher, Dwight S.] USDA ARS, J Phil Campbell Sr Nat Resource Conservat Ctr, Watkinsville, GA 30677 USA. [Newton, G. Larry] Univ Georgia, Dept Anim & Dairy Sci, Tifton, GA 31793 USA. [Vellidis, George] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31793 USA. RP Jenkins, MB (reprint author), USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Unit, Oxford, MS 38655 USA. EM michael.jenkins@ars.usda.gov OI Adams, Morgan/0000-0002-3602-6849 FU USDA-CSREES NRI Competitive Grants Program FX The authors are grateful for the expert technical support of Shaheen Humayoun, Stephen Norris, Anthony Dillard, Jessica Sterling, Michael Martin, Brooke Powell, Wynn Page, Debbie Coker and Chris Clegg. This research was supported in part by a grant from the USDA-CSREES NRI Competitive Grants Program. NR 28 TC 0 Z9 0 U1 3 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD JAN 31 PY 2015 VL 148 BP 97 EP 105 DI 10.1016/j.agwat.2014.09.022 PG 9 WC Agronomy; Water Resources SC Agriculture; Water Resources GA AW8XA UT WOS:000346541500011 ER PT J AU Day, JM Oakley, BB Seal, BS Zsak, L AF Day, J. Michael Oakley, Brian B. Seal, Bruce S. Zsak, Laszlo TI Comparative Analysis of the Intestinal Bacterial and RNA Viral Communities from Sentinel Birds Placed on Selected Broiler Chicken Farms SO PLOS ONE LA English DT Article ID INFECTIOUS-BRONCHITIS VIRUS; RUNTING-STUNTING SYNDROME; POULT ENTERITIS SYNDROME; MOLECULAR CHARACTERIZATION; MALABSORPTION-SYNDROME; TURKEY ASTROVIRUSES; GROWTH-PERFORMANCE; UNITED-STATES; PICOBIRNAVIRUS; PATHOGENESIS AB There is a great deal of interest in characterizing the complex microbial communities in the poultry gut, and in understanding the effects of these dynamic communities on poultry performance, disease status, animal welfare, and microbes with human health significance. Investigations characterizing the poultry enteric virome have identified novel poultry viruses, but the roles these viruses play in disease and performance problems have yet to be fully characterized. The complex bacterial community present in the poultry gut influences gut development, immune status, and animal health, each of which can be an indicator of overall performance. The present metagenomic investigation was undertaken to provide insight into the colonization of specific pathogen free chickens by enteric microorganisms under field conditions and to compare the pre-contact intestinal microbiome with the altered microbiome following contact with poultry raised in the field. Analysis of the intestinal virome from contact birds ("sentinels") placed on farms revealed colonization by members of the Picornaviridae, Picobirnaviridae, Reoviridae, and Astroviridae that were not present in pre-contact birds or present in proportionally lower numbers. Analysis of the sentinel gut bacterial community revealed an altered community in the post-contact birds, notably by members of the Lachnospiracea/Clostridium and Lactobacillus families and genera. Members of the avian enteric Reoviridae and Astroviridae have been well-characterized and have historically been implicated in poultry enteric disease; members of the Picobirnaviridae and Picornaviridae have only relatively recently been described in the poultry and avian gut, and their roles in the recognized disease syndromes and in poultry performance in general have not been determined. This metagenomic analysis has provided insight into the colonization of the poultry gut by enteric microbes circulating in commercial broiler flocks, and has identified enteric viruses and virus communities that warrant further study in order to understand their role(s) in avian gut health and disease. C1 [Day, J. Michael; Zsak, Laszlo] ARS, USDA, Southeast Poultry Res Lab, Athens, GA 30605 USA. [Oakley, Brian B.; Seal, Bruce S.] ARS, USDA, Poultry Microbiol Safety Res Unit, Athens, GA USA. RP Day, JM (reprint author), ARS, USDA, Southeast Poultry Res Lab, Athens, GA 30605 USA. EM Michael.day@ars.usda.gov FU USDA ARS CRIS project [6612-32000-067-00D]; United States Poultry and Egg Association Project [684] FX Funding was provided by the USDA ARS CRIS project 6612-32000-067-00D Intervention Strategies to Control and Prevent Enteric Viral Diseases of Poultry and the United States Poultry and Egg Association Project #684 Molecular Characterization of Novel Enteric Viruses Circulating in Poultry in the United States (http://www.uspoultry.org/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 69 TC 4 Z9 4 U1 5 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 30 PY 2015 VL 10 IS 1 AR e0117210 DI 10.1371/journal.pone.0117210 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CC9HX UT WOS:000350680700075 PM 25635690 ER PT J AU Brunelle, BW Bearson, BL Bearson, SMD AF Brunelle, Brian W. Bearson, Bradley L. Bearson, Shawn M. D. TI Chloramphenicol and tetracycline decrease motility and increase invasion and attachment gene expression in specific isolates of multidrug-resistant Salmonella enterica serovar Typhimurium SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE antibiotics; attachment; chloramphenicol; fimbriae; invasion; motility; Salmonella; tetracycline ID PATHOGENICITY ISLAND 1; III SECRETION SYSTEM; SUBINHIBITORY CONCENTRATIONS; SEROTYPE TYPHIMURIUM; FIMBRIAL OPERONS; STAPHYLOCOCCUS-AUREUS; BACTERIAL-RESISTANCE; TYPE-1 FIMBRIAE; MOLECULAR-BASIS; IN-VITRO AB Salmonella enterica serovar Typhimurium is one of the most common serovars isolated from humans and livestock, and over 35% of these isolates are resistant to three or more antibiotics. Multidrug-resistant (MDR) Salmonella is a public health concern as it is associated with increased morbidity in patients compared to antibiotic sensitive strains, though it is unknown how the antibiotic resistant isolates lead to a more severe infection. Cellular invasion is temporally regulated in Salmonella and normally occurs during late-log and stationary growth. However, our previous work determined that a 30 min exposure to a sub-inhibitory concentration of tetracycline can induce the full invasion phenotype during early-log growth in certain MDR S. Typhimurium isolates. The current study examined whether sub-inhibitory concentrations of other antibiotics could also induce the invasiveness in the same set of isolates. Ampicillin and streptomycin had no effect on invasion, but certain concentrations of chloramphenicol were found to induce invasion in a subset of isolates. Two of the isolates induced by chloramphenicol were also inducible by tetracycline. RNA-seq analyses demonstrated that chloramphenicol and tetracycline both down-regulated motility gene expression, while up-regulating genes associated with attachment, invasion, and intracellular survival. Eleven fimbrial operons were up-regulated, which is notable as only three fimbrial operons were thought to be inducible in culture; six of these up-regulated operons have been reported to play a role in Salmonella persistence in mice. Overall, these data show that the normal progression of the genetic pathways that regulate invasion can be expedited to occur within 30 min due to antibiotic exposure. This altered invasion process due to antibiotics may play a role in the increased intensity and duration of infection observed in patients with MDR Salmonella. C1 [Brunelle, Brian W.; Bearson, Shawn M. D.] USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Bearson, Bradley L.] USDA ARS, Agroecosyst Management Res Unit, Natl Lab Agr & Environm, Ames, IA 50010 USA. RP Brunelle, BW (reprint author), USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM brian.brunelle@ars.usda.gov FU USDA fund; ARS CRIS fund FX We would like to thank Briony Atkinson for her outstanding technical assistance, as well as Judith Stasko for her fantastic electron microscopy work. We would also like to thank Dr. Tracy Nicholson and Dr. Thomas Casey for their critical review of the manuscript. This research was supported by USDA, ARS CRIS funds. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 1 Z9 1 U1 5 U2 18 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD JAN 30 PY 2015 VL 6 AR 801 DI 10.3389/fmicb.2014.00801 PG 12 WC Microbiology SC Microbiology GA CA7NA UT WOS:000349102900001 ER PT J AU Buron-Moles, G Wisniewski, M Vinas, I Teixido, N Usall, J Droby, S Torres, R AF Buron-Moles, Gemma Wisniewski, Michael Vinas, Inmaculada Teixido, Neus Usall, Josep Droby, Samir Torres, Rosario TI Characterizing the proteome and oxi-proteome of apple in response to a host (Penicillium expansum) and a non-host (Penicillium digitatum) pathogen SO JOURNAL OF PROTEOMICS LA English DT Article DE Malus domestica; Green mold; Blue mold; Protein oxidation; RT-qPCR ID DIFFERENT MATURITY STAGES; DEFENSE-RELATED PROTEINS; SWEET CHERRY FRUIT; OXIDATIVE STRESS; SALICYLIC-ACID; TRANSCRIPTION FACTOR; HYDROGEN-PEROXIDE; BOTRYTIS-CINEREA; GENE FAMILY; RESISTANCE AB Apples are subjected to both abiotic and biotic stresses during the postharvest period, which lead to large economic losses worldwide. To obtain biochemical insights into apple defense response, we monitored the protein abundance changes (proteome), as well as the protein carbonyls (oxi-proteome) formed by reactive oxygen species (ROS) in 'Golden Smoothee' apple in response to wounding, Penicillium expansum (host) and Penicillium digitatum (non-host) pathogens with select transcriptional studies. To examine the biological relevance of the results, we described quantitative and oxidative protein changes into the gene ontology functional categories, as well as into de KEGG pathways. We identified 26 proteins that differentially changed in abundance in response to wounding, P. expansum or P. digitatum infection. While these changes showed some similarities between the apple responses and abiotic and biotic stresses, Mal d 1.03A case, other proteins as Mal d 1.03E and EF-Tu were specifically induced in response to P. digitatum infection. Using a protein carbonyl detection method based on fluorescent Bodipy, we detected and identified 27 oxidized proteins as sensitive ROS targets. These ROS target proteins were related to metabolism processes, suggesting that this process plays a leading role in apple fruit defense response against abiotic and biotic stresses. ACC oxidase and two glutamine synthetases showed the highest protein oxidation level in response to P. digitaturn infection. Documenting changes in the proteome and, specifically in oid-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. Possible mechanisms by which these modified proteins are involved in fruit defense response are discussed. Biological significance Mechanical damage in apple fruits is linked annually to large economic losses due to opportunistic infection by postharvest pathogens, such as P. expansum. Despite the current use of chemical fungicides and the implementation of new alternative strategies, blue mold remains a critical disease of these stored fruits worldwide. Actual trends are focused on acquiring the knowledge of the host-pathogen interactions because it may help on finding new rational and environmentally friendly control alternatives. Despite the economic importance of some postharvest diseases, proteomics has only been applied in a few cases to study fruit-pathogen interactions. On the one hand, this is the first study that monitored changes at the proteome and oxi-proteome level in 'Golden Smoothee' apple fruits in response to P. expansum (compatible) and P. digitaturn (non-host) pathogens. On the other hand, the main technological innovation of the reported research is the detection and quantification of oxidized (carbonylated) proteins to assess protein oxidative damage, avoiding the immunoblotting technique. The importance of the biological process investigated lies in the different mechanisms induced in fruit in response to P. expansurn and P. digitatum. Results revealed that fruit recognizes and reacts to P. expansurn in a similar manner to wounding, while its response to P. digitatum exhibits few differences in the protein profile. Documenting changes in the proteome and, specifically in md-proteome of apple can provide information that can be used to better understand how impaired protein functions may affect apple defense mechanisms. It also provides new biomarkers for oxidative damage mainly caused by the oxidative response occurring in fruit tissue in response to a host and a non-host pathogen. (C) 2014 Elsevier B.V. All rights reserved. C1 [Buron-Moles, Gemma; Vinas, Inmaculada] Lleida Univ, Dept Food Technol, XaRTA Postharvest, Agrotecnio Ctr, Lleida 25198, Catalonia, Spain. [Wisniewski, Michael] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Teixido, Neus; Usall, Josep; Torres, Rosario] IRTA, XaRTA Postharvest, Lleida 25198, Catalonia, Spain. [Droby, Samir] ARO, Volcani Ctr, Dept Postharvest Sci, IL-50250 Bet Dagan, Israel. RP Torres, R (reprint author), IRTA, XaRTA Postharvest, Rouira Roure 191, Lleida 25198, Catalonia, Spain. EM rosario.torres@irta.cat RI Teixido, Neus/D-2487-2017; OI Teixido, Neus/0000-0002-1676-3593; Buron-Moles, Gemma/0000-0002-3799-3339 FU Spanish Government [AGL2011-30519-CO3-03, BES-2009-027752] FX Authors are grateful to the Spanish Government for its financial support with the project AGL2011-30519-CO3-03, and for the scholarship BES-2009-027752 (G. Buron-Moles). The authors are indebted to Dr. Pablo Librado from 'Departament de Genetica. UB' for the bioinformatician support and to Celia Sanchez from 'IRTA' for her excellent technical assistance. We also would like to thank Dr. Isabel Sanchez and Dr. M. Alba Sorolla from the 'Servei de Genomica i Proteomica; UdL' for helping on peptide mass fingerprinting and MS-MS. Erik Burchard is acknowledged for his assistance in conducting the RT-qPCR experiments. NR 57 TC 4 Z9 4 U1 5 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD JAN 30 PY 2015 VL 114 BP 136 EP 151 DI 10.1016/j.jprot.2014.11.007 PG 16 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA CA5LE UT WOS:000348949100012 PM 25464364 ER PT J AU Wang, CC McAlindon, T Fielding, RA Harvey, WF Driban, JB Price, LL Kalish, R Schmid, A Scott, TM Schmid, CH AF Wang, Chenchen McAlindon, Timothy Fielding, Roger A. Harvey, William F. Driban, Jeffrey B. Price, Lori Lyn Kalish, Robert Schmid, Anna Scott, Tammy M. Schmid, Christopher H. TI A novel comparative effectiveness study of Tai Chi versus aerobic exercise for fibromyalgia: study protocol for a randomized controlled trial SO TRIALS LA English DT Article DE Tai Chi; Aerobic exercise; Fibromyalgia; Randomized controlled trial; Comparative effectiveness research ID TREATING KNEE OSTEOARTHRITIS; RHEUMATOID-ARTHRITIS; PHYSICAL-ACTIVITY; LIMITED ELDERS; 6-MINUTE WALK; PAIN SEVERITY; MUSCLE POWER; BALANCE TEST; OLDER WOMEN; LEG BALANCE AB Background: Fibromyalgia is a chronic musculoskeletal pain syndrome that causes substantial physical and psychological impairment and costs the US healthcare system over $25 billion annually. Current pharmacological therapies may cause serious adverse effects, are expensive, and fail to effectively improve pain and function. Finding new and effective non-pharmacological treatments for fibromyalgia patients is urgently needed. We are currently conducting the first comparative effectiveness randomized trial of Tai Chi versus aerobic exercise (a recommended component of the current standard of care) in a large fibromyalgia population. This article describes the design and conduct of this trial. Methods/design: A single-center, 52-week, randomized controlled trial of Tai Chi versus aerobic exercise is being conducted at an urban tertiary medical center in Boston, Massachusetts. We plan to recruit 216 patients with fibromyalgia. The study population consists of adults >= 21 years of age with fibromyalgia who meet American College of Rheumatology 1990 and 2010 diagnostic criteria. Participants are randomized to one of four Tai Chi intervention groups: 12 or 24 weeks of supervised Tai Chi held once or twice per week, or a supervised aerobic exercise control held twice per week for 24 weeks. The primary outcome is the change in Revised Fibromyalgia Impact Questionnaire total score from baseline to 24 weeks. Secondary outcomes include measures of widespread pain, symptom severity, functional performance, balance, muscle strength and power, psychological functioning, sleep quality, self efficacy, durability effects, and health related quality of life at 12, 24, and 52 week follow up. Discussion: This study is the first comparative effectiveness randomized trial of Tai Chi versus aerobic exercise in a large fibromyalgia population with long-term follow up. We present here a robust and well-designed trial to determine the optimal frequency and duration of a supervised Tai Chi intervention with regard to short- and long-term effectiveness. The trial also explores multiple outcomes to elucidate the potential mechanisms of Tai Chi and aerobic exercise and the generalizability of these interventions across instructors. Results of this study are expected to have important public health implications for patients with a major disabling disease that incurs substantial health burdens and economic costs. C1 [Wang, Chenchen; McAlindon, Timothy; Harvey, William F.; Driban, Jeffrey B.; Kalish, Robert; Schmid, Anna] Tufts Univ, Sch Med, Tufts Med Ctr, Ctr Complementary & Integrat Med,Div Rheumatol, Boston, MA 02111 USA. [Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Price, Lori Lyn] Tufts Univ, Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Tufts Clin & Translat Sci Inst, Boston, MA 02111 USA. [Scott, Tammy M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Neurosci & Aging Lab, Boston, MA 02111 USA. [Schmid, Christopher H.] Brown Univ, Sch Publ Hlth, Dept Biostat, Providence, RI 02912 USA. [Schmid, Christopher H.] Brown Univ, Sch Publ Hlth, Ctr Evidence Based Med, Providence, RI 02912 USA. RP Wang, CC (reprint author), Tufts Univ, Sch Med, Tufts Med Ctr, Ctr Complementary & Integrat Med,Div Rheumatol, Boston, MA 02111 USA. EM CWang2@tuftsmedicalcenter.org OI Schmid, Christopher/0000-0002-0855-5313 FU National Center for Complementary and Intergrative Health (NCCIH) of the National Institutes of Health [R01 AT006367]; National Center for Research Resources, National Institutes of Health [UL1 RR025752]; National Center for Advancing Translational Sciences, National Institutes of Health [UL1TR000073, UL1TR001064]; US Department of Agriculture [58-1950-0-014]; Boston Claude D Pepper Older Americans Independence Center [1P30AG031679]; Boston Rehabilitation Outcomes Center [1R24HD065688-01A1] FX CW is supported by R01 AT006367 from the National Center for Complementary and Intergrative Health (NCCIH) of the National Institutes of Health. The research is also supported from the National Center for Research Resources, National Institutes of Health, Grant Number UL1 RR025752 and the National Center for Advancing Translational Sciences, National Institutes of Health, Award Numbers UL1TR000073 and UL1TR001064. The contents of this manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the NCCIH or the National Institutes of Health. RAF's work on this project was partially supported by the US Department of Agriculture, under agreement No. 58-1950-0-014 and the Boston Claude D Pepper Older Americans Independence Center (1P30AG031679) and the Boston Rehabilitation Outcomes Center (1R24HD065688-01A1). Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the US Department of Agriculture. The investigators are solely responsible for the content of the manuscript and the decision to submit for publication. The authors gratefully acknowledge the Data Safety Monitoring Board members, Drs Yvonne Lee, Kristin Baker and Yulei He, for their insightful suggestions and comments for the study protocol. We thank the Clinical and Translational Research Center nurses for their help with various aspects of the study and Ramel Rones, Dorri Li, Brian Muccio for their expertise in teaching the Tai Chi groups as well as Kimberly Martin, Eleni Tsiroyannis, Erica McDavitt, Julie Krol, Rachel Felson and Ling Chu for their Aerobic Exercise teaching, and the study participants, whose cooperation, encouragement, and enthusiasm are an inspiration to us. NR 73 TC 6 Z9 6 U1 7 U2 49 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1745-6215 J9 TRIALS JI Trials PD JAN 30 PY 2015 VL 16 AR 34 DI 10.1186/s13063-015-0548-x PG 13 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA CA7VX UT WOS:000349126600001 PM 25633475 ER PT J AU Andreasen, A Petersen, HH Kringel, H Iburg, TM Skovgaard, K Dawson, H Urban, JF Thamsborg, SM AF Andreasen, Annette Petersen, Heidi H. Kringel, Helene Iburg, Tine M. Skovgaard, Kerstin Dawson, Harry Urban, Joseph F., Jr. Thamsborg, Stig M. TI Immune and inflammatory responses in pigs infected with Trichuris suis and Oesophagostomum dentatum SO VETERINARY PARASITOLOGY LA English DT Article DE Pig; Trichuris suis; Oesophagostomum dentatum; Helminth co-infection; Immune response; qPCR ID ALTERNATIVELY ACTIVATED MACROPHAGES; POPULATION-DYNAMICS; HELMINTH INFECTIONS; GASTROINTESTINAL NEMATODES; TRICHINELLA-SPIRALIS; PROTECTIVE IMMUNITY; GENE-EXPRESSION; IL-4 RESPONSES; ASCARIS-SUUM; PARASITES AB The aim of the present study was to investigate parasite induced immune responses in pigs co-infected with Trichuris suis and Oesophagostomum dentatum as compared to monospecies infected pigs. T. suis is known to elicit a strong immune response leading to rapid expulsion, and a strong antagonistic effect on O. dentatum populations has been observed in co-infected pigs. Forty-eight helminth naive pigs were allocated into 4 groups in a 2-factorial design. Two groups were trickle inoculated with either 10 T. suis eggs/kg/day (Group T) or 20 0. dentatum L3/kg/day (Group 0). Group OT was infected with same levels of both T. suis and 0. dentatum (Group OT) and Group C remained uninfected. In each group, six pigs were necropsied after 35 days and the remaining pigs after 71 days. Parasite E/S-antigen specific serum antibodies were quantified by an in-direct ELISA. qPCR was used to measure the expression of immune function related genes in the mucosa of proximal colon and the draining lymph node. Highly significant interactions were identified for O. dentatum specific IgG1 (p < 0.0001) and IgG2 (p < 0.0006) antibodies with a remarkable 2-fold higher antibody response in group OT pigs as compared to group 0. These findings indicated that T. suis enhanced the antibody response against 0. dentatum in Group OT. The gene expression data confirmed a strong Type 2 response to T. suis (e.g. marked increase in IL-13, ARG1 and CCL11) and clearly weaker in amplitude and/or delayed onset response to 0. dentatum in the single infected group. Interactions were found between the two nematodes with regard to several cytokines, e.g. the increase in IL-13 observed in Group T was absent in Group OT (p = 0.06, proximal colon mucosa, 35 and 71 p.i.). Some of these immune response-related interactions may support, or even partially explain, the observed interactions between the two worm populations in co-infected pigs. (C) 2014 Elsevier B.V. All rights reserved. C1 [Andreasen, Annette; Petersen, Heidi H.; Kringel, Helene; Thamsborg, Stig M.] Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet Dis Biol, DK-1870 Frederiksberg C, Denmark. [Iburg, Tine M.] Natl Vet Inst, Dept Pathol & Wildlife Dis, SE-75189 Uppsala, Sweden. [Skovgaard, Kerstin] Tech Univ Denmark, Natl Vet Inst, Innate Immunol Grp, DK-1870 Frederiksberg C, Denmark. [Dawson, Harry; Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Gen & Immunol Lab, Beltsville, MD 20705 USA. RP Andreasen, A (reprint author), Univ Copenhagen, Fac Hlth & Med Sci, Dept Vet Dis Biol, Dyrlaegevej 100 Bldg 1-05 3, DK-1870 Frederiksberg C, Denmark. EM malte@sund.ku.dk OI Urban, Joseph/0000-0002-1590-8869; skovgaard, kerstin/0000-0001-5663-4879 NR 52 TC 5 Z9 5 U1 1 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JAN 30 PY 2015 VL 207 IS 3-4 BP 249 EP 258 DI 10.1016/j.vetpar.2014.12.005 PG 10 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA CA9UU UT WOS:000349271100007 PM 25576439 ER PT J AU Al-Hamdan, OZ Hernandez, M Pierson, FB Nearing, MA Williams, CJ Stone, JJ Boll, J Weltz, MA AF Al-Hamdan, Osama Z. Hernandez, Mariano Pierson, Frederick B. Nearing, Mark A. Williams, C. Jason Stone, Jeffrey J. Boll, Jan Weltz, Mark A. TI Rangeland hydrology and erosion model (RHEM) enhancements for applications on disturbed rangelands SO HYDROLOGICAL PROCESSES LA English DT Article DE erosion modelling; fire; woodland encroachment; rangeland management; dynamic erodibility; stream power ID SOIL-EROSION; PRESCRIBED-FIRE; JUNIPER ENCROACHMENT; CONCENTRATED FLOW; WATER REPELLENCY; WESTERN JUNIPER; SHEAR-STRESS; INFILTRATION; RUNOFF; ECOSYSTEMS AB The rangeland hydrology and erosion model (RHEM) is a new process-based model developed by the USDA Agricultural Research Service. RHEM was initially developed for functionally intact rangelands where concentrated flow erosion is minimal and most soil loss occurs by rain splash and sheet flow erosion processes. Disturbance such as fire or woody plant encroachment can amplify overland flow erosion by increasing the likelihood of concentrated flow formation. In this study, we enhanced RHEM applications on disturbed rangelands by using a new approach for the prediction and parameterization of concentrated flow erosion. The new approach was conceptualized based on observations and results of experimental studies on rangelands disturbed by fire and/or by tree encroachment. The sediment detachment rate for concentrated flow was calculated using soil erodibility and hydraulic (flow width and stream power) parameters. Concentrated flow width was calculated based on flow discharge and slope using an equation developed specifically for disturbed rangelands. Soil detachment was assumed to begin with concentrated flow initiation. A dynamic erodibility concept was applied where concentrated flow erodibility was set to decrease exponentially during a run-off event because of declining sediment availability. Erodibility was estimated using an empirical parameterization equation as a function of vegetation cover and surface soil texture. A dynamic partial differential sediment continuity equation was used to model the total detachment rate of concentrated flow and rain splash and sheet flow. The enhanced version of the model was evaluated against rainfall simulation data for three different sites that exhibit some degree of disturbance by fire and/or by tree encroachment. The coefficient of determination (R-2) and Nash-Sutcliffe efficiency were 0.78 and 0.71, respectively, which indicates the capability of the model using the new approach for predicting soil loss on disturbed rangeland. By using the new concentrated flow modelling approach, the model was enhanced to be a practical tool that utilizes readily available vegetation and soil data for quantifying erosion and assessing erosion risk following rangeland disturbance. Copyright (C) 2014 John Wiley & Sons, Ltd. C1 [Al-Hamdan, Osama Z.; Pierson, Frederick B.; Williams, C. Jason] ARS, USDA, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Al-Hamdan, Osama Z.; Boll, Jan] Univ Idaho, Dept Biol & Agr Engn, Moscow, ID 83843 USA. [Hernandez, Mariano; Nearing, Mark A.; Stone, Jeffrey J.] ARS, Southwest Watershed Res Ctr, USDA, Tucson, AZ USA. [Hernandez, Mariano] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Weltz, Mark A.] USDA, Great Basin Rangelands Res Unit, Reno, NV USA. RP Al-Hamdan, OZ (reprint author), ARS, USDA, Northwest Watershed Res Ctr, Boise, ID 83712 USA. EM osama.al-hamdan@ars.usda.gov OI Williams, Jason/0000-0002-6289-4789 FU USDA-NRCS/ARS Conservation Effects Assessment Project; US Joint Fire Science Program FX This research was funded in part by the USDA-NRCS/ARS Conservation Effects Assessment Project and the US Joint Fire Science Program. This paper is contribution No. 94 of the Sagebrush Steppe Treatment Evaluation Project, funded by the US Joint Fire Science Program. The USDA is an equal opportunity provider and employer. NR 47 TC 8 Z9 9 U1 1 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD JAN 30 PY 2015 VL 29 IS 3 BP 445 EP 457 DI 10.1002/hyp.10167 PG 13 WC Water Resources SC Water Resources GA AY9CM UT WOS:000347847300009 ER PT J AU Register, KB Thole, L Rosenbush, RF Minion, FC AF Register, Karen B. Thole, Luke Rosenbush, Ricardo F. Minion, F. Chris TI Multi locus sequence typing of Mycoplasma bovis reveals host-specific genotypes in cattle versus bison SO VETERINARY MICROBIOLOGY LA English DT Article DE Mycoplasma bovis; Multilocus sequence typing; Bison; Cattle ID TANDEM-REPEAT ANALYSIS; MOLECULAR EPIDEMIOLOGIC ANALYSIS; FIELD GEL-ELECTROPHORESIS; POLYMERASE-CHAIN-REACTION; MASTITIS; HERD; EVOLUTIONARY; INVESTIGATE; INFECTIONS; VALIDATION AB Mycoplasma bovis is a primary agent of mastitis, pneumonia and arthritis in cattle and the bacterium most frequently isolated from the polymicrobial syndrome known as bovine respiratory disease complex. Recently, M. bovis has emerged as a significant health problem in bison, causing necrotic pharyngitis, pneumonia, dystocia and abortion. Whether isolates from cattle and bison comprise genetically distinct populations is unknown. This study describes the development of a highly discriminatory multilocus sequencing typing (MLST) method for M. bovis and its use to investigate the population structure of the bacterium. Genome sequences from six M. bovis isolates were used for selection of gene targets. Seven of 44 housekeeping genes initially evaluated were selected as targets on the basis of sequence variability and distribution within the genome. For each gene target sequence, four to seven alleles could be distinguished that collectively define 32 sequence types (STs) from a collection of 94 cattle isolates and 42 bison isolates. A phylogeny based on concatenated target gene sequences of each isolate revealed that bison isolates are genetically distinct from strains that infect cattle, suggesting recent disease outbreaks in bison may be due to the emergence of unique genetic variants. No correlation was found between ST and disease presentation or geographic origin. MLST data reported here were used to populate a newly created and publicly available, curated database to which researchers can contribute. The MLST scheme and database provide novel tools for exploring the population structure of M. bovis and tracking the evolution and spread of strains. Published by Elsevier B.V. C1 [Register, Karen B.] USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. [Thole, Luke; Rosenbush, Ricardo F.; Minion, F. Chris] Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. RP Register, KB (reprint author), USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM karen.register@ars.usda.gov FU Merial Veterinary Scholars Program; Iowa Livestock Health Advisory Council FX We wish to thank David Alt and Lea Ann Hobbs for DNA sequence data and Timothy Frana, Inna Lysnyansky, A.W. Layton, Shelagh Copeland, Neil Dyer, David Miskimins, David Hunter, Jose Perez-Casal and Robert Briggs for the gift of M. bovis isolates. L. Thole was supported by funds from the Merial Veterinary Scholars Program. Additional funding, in part, was provided by the Iowa Livestock Health Advisory Council. NR 39 TC 10 Z9 10 U1 2 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD JAN 30 PY 2015 VL 175 IS 1 BP 92 EP 98 DI 10.1016/j.vetmic.2014.11.002 PG 7 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA AZ2SQ UT WOS:000348083100012 PM 25433454 ER PT J AU Taylor-Teeples, M Lin, L de Lucas, M Turco, G Toal, TW Gaudinier, A Young, NF Trabucco, GM Veling, MT Lamothe, R Handakumbura, PP Xiong, G Wang, C Corwin, J Tsoukalas, A Zhang, L Ware, D Pauly, M Kliebenstein, DJ Dehesh, K Tagkopoulos, I Breton, G Pruneda-Paz, JL Ahnert, SE Kay, SA Hazen, SP Brady, SM AF Taylor-Teeples, M. Lin, L. de Lucas, M. Turco, G. Toal, T. W. Gaudinier, A. Young, N. F. Trabucco, G. M. Veling, M. T. Lamothe, R. Handakumbura, P. P. Xiong, G. Wang, C. Corwin, J. Tsoukalas, A. Zhang, L. Ware, D. Pauly, M. Kliebenstein, D. J. Dehesh, K. Tagkopoulos, I. Breton, G. Pruneda-Paz, J. L. Ahnert, S. E. Kay, S. A. Hazen, S. P. Brady, S. M. TI An Arabidopsis gene regulatory network for secondary cell wall synthesis SO NATURE LA English DT Article ID VASCULAR-RELATED NAC-DOMAIN6; XYLEM VESSEL FORMATION; TRANSCRIPTION FACTORS; FUNCTIONAL GENOMICS; CIRCADIAN CLOCK; EXPRESSION; ROOT; IDENTIFICATION; PATHWAY; MYB46 AB The plant cell wall is an important factor for determining cell shape, function and response to the environment. Secondary cell walls, such as those found in xylem, are composed of cellulose, hemicelluloses and lignin and account for the bulk of plant biomass. The coordination between transcriptional regulation of synthesis for each polymer is complex and vital to cell function. A regulatory hierarchy of developmental switches has been proposed, although the full complement of regulators remains unknown. Here we present a protein-DNA network between Arabidopsis thaliana transcription factors and secondary cell wall metabolic genes with gene expression regulated by a series of feed-forward loops. This model allowed us to develop and validate new hypotheses about secondary wall gene regulation under abiotic stress. Distinct stresses are able to perturb targeted genes to potentially promote functional adaptation. These interactions will serve as a foundation for understanding the regulation of a complex, integral plant component. C1 [Taylor-Teeples, M.; de Lucas, M.; Turco, G.; Toal, T. W.; Gaudinier, A.; Wang, C.; Dehesh, K.; Brady, S. M.] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. [Taylor-Teeples, M.; de Lucas, M.; Turco, G.; Toal, T. W.; Gaudinier, A.; Tsoukalas, A.; Tagkopoulos, I.; Brady, S. M.] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Lin, L.; Young, N. F.; Trabucco, G. M.; Veling, M. T.; Lamothe, R.; Handakumbura, P. P.; Hazen, S. P.] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. [Xiong, G.; Pauly, M.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Corwin, J.; Kliebenstein, D. J.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Tsoukalas, A.; Tagkopoulos, I.] Univ Calif Davis, Dept Comp Sci, Davis, CA 95616 USA. [Zhang, L.; Ware, D.] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Ware, D.] ARS, USDA, Ithaca, NY 14853 USA. [Breton, G.; Pruneda-Paz, J. L.; Kay, S. A.] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA. [Ahnert, S. E.] Univ Cambridge, Cavendish Lab, Theory Condensed Matter Grp, Cambridge CB3 0HE, England. RP Brady, SM (reprint author), Univ Calif Davis, Dept Plant Biol, One Shields Ave, Davis, CA 95616 USA. EM hazen@bio.umass.edu; sbrady@ucdavis.edu RI Breton, Ghislain/F-7266-2016; Pauly, Markus/B-5895-2008 OI Breton, Ghislain/0000-0001-6400-3858; Pauly, Markus/0000-0002-3116-2198 FU Office of Science (BER) Department of Energy [DE-FG02-08ER64700DE]; National Institute of General Medical Sciences of the National Institutes of Health [RO1GM056006, RC2GM092412]; National Institute of Health [RO1GM107311]; National Science Foundation [IOS-1036491, IOS-1352478]; USDA CRIS [1907-21000-030]; Royal Society UK Fellowship; UC Davis Startup Funds; Hellman Fellowship FX We thank M. Tierney (University of Vermont) for 35S::GFP seeds, T. Demura for VND7 resources, M.K. Barton for REV:GR seeds, E.P. Spalding for advice on manuscript revision, and C. Gutierrez for E2Fc RNAi and E2Fc N-terminal deletion overexpressor seeds and useful discussion. This research was supported by the Office of Science (BER) Department of Energy Grant DE-FG02-08ER64700DE (to S.P.H. and S.A.K.), National Institute of General Medical Sciences of the National Institutes of Health under award numbers RO1GM056006 and RC2GM092412 (to S.A.K.), National Institute of Health (RO1GM107311) and National Science Foundation (IOS-1036491 and IOS-1352478) to K.D., USDA CRIS 1907-21000-030 to D.W. and L.F., a Royal Society UK Fellowship (to SEA.), and UC Davis Startup Funds and a Hellman Fellowship (to S.M.B). NR 53 TC 70 Z9 71 U1 41 U2 218 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD JAN 29 PY 2015 VL 517 IS 7536 BP 571 EP U307 DI 10.1038/nature14099 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA2ZL UT WOS:000348775000034 PM 25533953 ER PT J AU Monleon, VJ Lintz, HE AF Monleon, Vicente J. Lintz, Heather E. TI Evidence of Tree Species' Range Shifts in a Complex Landscape SO PLOS ONE LA English DT Article ID DRIVE DOWNHILL SHIFTS; CLIMATE-CHANGE; OPTIMUM ELEVATIONS; UNITED-STATES; MIGRATION; FORESTS; MORTALITY; RICHNESS; 20TH-CENTURY; BUTTERFLIES AB Climate change is expected to change the distribution of species. For long-lived, sessile species such as trees, tracking the warming climate depends on seedling colonization of newly favorable areas. We compare the distribution of seedlings and mature trees for all but the rarest tree species in California, Oregon and Washington, United States of America, a large, environmentally diverse region. Across 46 species, the mean annual temperature of the range of seedlings was 0.120 degrees C colder than that of the range of trees (95% confidence interval from 0.096 to 0.144 degrees C). The extremes of the seedling distributions also shifted towards colder temperature than those of mature trees, but the change was less pronounced. Although the mean elevation and mean latitude of the range of seedlings was higher than and north of those of the range of mature trees, elevational and latitudinal shifts run in opposite directions for the majority of the species, reflecting the lack of a direct biological relationship between species' distributions and those variables. The broad scale, environmental diversity and variety of disturbance regimes and land uses of the study area, the large number and exhaustive sampling of tree species, and the direct causal relationship between the temperature response and a warming climate, provide strong evidence to attribute the observed shifts to climate change. C1 [Monleon, Vicente J.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Lintz, Heather E.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Oregon Climate Change Res Inst, Corvallis, OR 97331 USA. RP Monleon, VJ (reprint author), US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. EM vjmonleon@fs.fed.us FU Oregon Department of Forestry FX HEL received partial funding from the Oregon Department of Forestry. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 44 TC 13 Z9 13 U1 9 U2 42 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 29 PY 2015 VL 10 IS 1 AR UNSP e0118069 DI 10.1371/journal.pone.0118069 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA0LR UT WOS:000348609800015 PM 25634090 ER PT J AU Kim, J Chang, C Tucker, ML AF Kim, Joonyup Chang, Caren Tucker, Mark L. TI To grow old: regulatory role of ethylene and jasmonic acid in senescence SO Frontiers in Plant Science LA English DT Review DE ethylene; jasmonic acid; leaf senescence; cross-talk; miRNA; transcription factors ID ARABIDOPSIS LEAF SENESCENCE; F-BOX PROTEINS; RESPONSE PATHWAY; NICOTIANA-SYLVESTRIS; TRANSCRIPTION FACTOR; MICRORNA REGULATION; GENE; EIN2; MUTANTS; EXPRESSION AB Senescence, the final stage in the development of an organ or whole plant, is a genetically programmed process controlled by developmental and environmental signals. Age-related signals underlie the onset of senescence in specific organs (leaf, flower, and fruit) as well as the whole plant (monocarpic senescence). Rudimentary to most senescence processes is the plant hormone ethylene, a small gaseous molecule critical to diverse processes throughout the life of the plant. The role of ethylene in senescence was discovered almost 100 years ago, but the molecular mechanisms by which ethylene regulates senescence have been deciphered more recently primarily through genetic and molecular studies in Arabidopsis. Jasmonic acid (JA), another plant hormone, is emerging as a key player in the control of senescence. The regulatory network of ethylene and JA involves the integration of transcription factors, microRNAs, and other hormones. In this review, we summarize the current understanding of ethylene's role in senescence, and discuss the interplay of ethylene with JA in the regulation of senescence. C1 [Kim, Joonyup; Tucker, Mark L.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Kim, Joonyup; Chang, Caren] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. RP Kim, J (reprint author), USDA ARS, Soybean Genom & Improvement Lab, 10300 Baltimore Ave,Bldg 006,Room 212, Beltsville, MD 20705 USA. EM joonyup.kim@ars.usda.gov; mark.tucker@ars.usda.gov FU Binational Agricultural and Development Fund (BARD) [US-4571-12C]; UMD Libraries Open Access Publishing Fund FX This work was supported by a Binational Agricultural and Development Fund (BARD) US-4571-12C grant to Mark L. Tucker, and funding for Open Access was provided by the UMD Libraries Open Access Publishing Fund. NR 74 TC 12 Z9 13 U1 17 U2 115 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JAN 29 PY 2015 VL 6 AR 20 DI 10.3389/fpls.2015.00020 PG 7 WC Plant Sciences SC Plant Sciences GA CA2EC UT WOS:000348720700001 PM 25688252 ER PT J AU Poelchau, M Childers, C Moore, G Tsavatapalli, V Evans, J Lee, CY Lin, H Lin, JW Hackett, K AF Poelchau, Monica Childers, Christopher Moore, Gary Tsavatapalli, Vijaya Evans, Jay Lee, Chien-Yueh Lin, Han Lin, Jun-Wei Hackett, Kevin TI The i5k Workspace@NAL-enabling genomic data access, visualization and curation of arthropod genomes SO NUCLEIC ACIDS RESEARCH LA English DT Article ID ANOPHELES-GAMBIAE; APIS-MELLIFERA; INSECT GENOMES; GENERATION; ANNOTATION; EVOLUTION AB The 5000 arthropod genomes initiative (i5k) has tasked itself with coordinating the sequencing of 5000 insect or related arthropod genomes. The resulting influx of data, mostly from small research groups or communities with little bioinformatics experience, will require visualization, dissemination and curation, preferably from a centralized platform. The National Agricultural Library (NAL) has implemented the i5kWorkspace@NAL (http://i5k.nal.usda.gov/) to help meet the i5k initiative's genome hosting needs. Any i5k member is encouraged to contact the i5k Workspace with their genome project details. Once submitted, new content will be accessible via organism pages, genome browsers and BLAST search engines, which are implemented via the open-source Tripal framework, a web interface for the underlying Chado database schema. We also implement the Web Apollo software for groups that choose to curate gene models. New content will add to the existing body of 35 arthropod species, which include species relevant for many aspects of arthropod genomic research, including agriculture, invasion biology, systematics, ecology and evolution, and developmental research. C1 [Poelchau, Monica; Childers, Christopher; Moore, Gary; Tsavatapalli, Vijaya; Lee, Chien-Yueh; Lin, Han; Lin, Jun-Wei] Natl Agr Lib, Beltsville, MD 20705 USA. [Evans, Jay] ARS, Bee Res Lab, USDA, Beltsville, MD 20705 USA. [Lee, Chien-Yueh; Lin, Han] Natl Taiwan Univ, Grad Inst Biomed Elect & Bioinformat, Taipei 10617, Taiwan. [Lin, Jun-Wei] Natl Taiwan Univ, Grad Inst Elect Engn, Taipei 10617, Taiwan. [Hackett, Kevin] ARS, USDA, Beltsville, MD 20705 USA. RP Poelchau, M (reprint author), Natl Agr Lib, Beltsville, MD 20705 USA. EM monica.poelchau@ars.usda.gov; christopher.childers@ars.usda.gov OI Childers, Chris/0000-0002-1253-5550; Evans, Jay/0000-0002-0036-4651 FU United States Department of Agriculture-Agricultural Research Service; Office of National Programs; Bee Research Laboratory; National Agricultural Library, United States Department of Agriculture-Agricultural Research Service FX United States Department of Agriculture-Agricultural Research Service; Office of National Programs; Bee Research Laboratory. Funding for open access charge: National Agricultural Library, United States Department of Agriculture-Agricultural Research Service. NR 21 TC 17 Z9 17 U1 4 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD JAN 28 PY 2015 VL 43 IS D1 BP D714 EP D719 DI 10.1093/nar/gku983 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CC2YF UT WOS:000350210400104 PM 25332403 ER PT J AU Lee, ST Cook, D Davis, TZ Gardner, DR Johnson, RL Stonecipher, CA AF Lee, Stephen T. Cook, Daniel Davis, T. Zane Gardner, Dale R. Johnson, Robert L. Stonecipher, Clinton A. TI A Survey of Tremetone, Dehydrotremetone, and Structurally Related Compounds in Isocoma spp. (Goldenbush) in the Southwestern United States SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE tremetone; dehydrotremetone; Isocoma pluriflora; Isocoma tenuisecta; Isocoma azteca; Isocoma acradenia; Isocoma rusbyi ID SNAKEROOT AGERATINA-ALTISSIMA; RAYLESS GOLDENROD; EUPATORIUM URTICAEFOLIUM; PLURIFLORA TOXICOSIS; COUMARINS; GOATS; CONSTITUENTS; TREMBLES; KETONES AB Isocoma pluriflora, a plant prevalent on land used for livestock production and native to Arizona, New Mexico, West Texas, and Northern Mexico, is poisonous and causes trembles in livestock. Tremetone and dehydrotremetone have been suggested as the toxic compounds in I. pluriflora. In this study several different Isocoma spp., including I. pluriflora, I. tenuisecta, I. azteca, I. acradenia, and I. rusbyi, that are native to land used for grazing livestock in the southwestern United States were analyzed by high performance liquid chromatography (HPLC) for tremetone, dehydrotremetone, and other structurally related compounds. This is the first report of tremetone, dehydrotremetone, and 3-oxyangeloyltremetone in I. tenuisecta, I. azteca, I. acradenia, I. rusbyi, and several other Isocoma spp. In addition, this is the first report of 4-hydroxy-3-(3-methyl-2-butenyl)acetophenone and 7-isopentenyloxycoumarin in Isocoma spp. C1 [Lee, Stephen T.; Cook, Daniel; Davis, T. Zane; Gardner, Dale R.; Stonecipher, Clinton A.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Johnson, Robert L.] Brigham Young Univ, Stanley L Welsh Herbarium, Provo, UT 84602 USA. RP Lee, ST (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 East 1400 North, Logan, UT 84341 USA. EM stephen.lee@ars.usda.gov NR 27 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 28 PY 2015 VL 63 IS 3 BP 872 EP 879 DI 10.1021/jf505423h PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CA1SU UT WOS:000348692600016 PM 25554830 ER PT J AU Zhang, L Lin, Q Feng, YZ Fan, XM Zou, F Yuan, DY Zeng, XC Cao, HP AF Zhang, Lin Lin, Qing Feng, Yanzhi Fan, Xiaoming Zou, Feng Yuan, De-Yi Zeng, Xiaochun Cao, Heping TI Transcriptomic Identification and Expression of Starch and Sucrose Metabolism Genes in the Seeds of Chinese Chestnut (Castanea mollissima) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Chinese chestnut (Castanea mollissima); next generation sequencing; quantitative real-time PCR; starch synthesis ID AMYLOPLAST MEMBRANE POLYPEPTIDES; MAIZE ENDOSPERM; ADP-GLUCOSE; ADENYLATE TRANSLOCATOR; SATIVA MILL.; RNA-SEQ; BIOSYNTHESIS; PURIFICATION; CULTIVARS; BRITTLE-1 AB The Chinese chestnut (Castanea mollissima) seed provides a rich source of carbohydrates as food and feed. However, little is known about starch biosynthesis in the seeds. The objectives of this study were to determine seed composition profiles and identify genes involved in starch and sucrose metabolism. Metabolite analysis showed that starch was the major component and rapidly accumulated during seed endosperm development. Amylopectin was approximately 3-fold of amylose content in chestnut starch. Illumina platform-based transcriptome sequencing generated 56671 unigenes in two cDNA libraries from seed endosperms collected at 45 and 75 days after flowering (DAF). A total of 1537 unigenes showed expression differences =2-fold in the two stages of seeds including 570 up-regulated and 967 down-regulated unigenes. One hundred and fifty-two unigenes were identified as involved in starch and sucrose metabolism, including 1 for glycogenin glucosyltransferase, 4 for adenylate transporter (brittle1-type), 3 for ADP-glucose pyrophosphorylase (AGP, not brittle2- or shrunken2-type), 3 for starch synthase (SS), 2 for starch branching enzyme, 5 for starch debranching enzyme, 11 for sucrose synthase, and 3 for sucrose-phosphate synthase. Among them, 58 unigenes showed a =2-fold expression difference between the 45 and 75 DAF seeds including 11 up- and 47 down-regulated unigenes. The expression of 21 unigenes putatively coding for major enzymes in starch and sucrose metabolism was validated by qPCR using RNA from five seed stages. Expression profiles and correlation analysis indicated that the mRNA levels of AGP (large and small subunits), granule-bound SS2, and soluble SS1 and SS4 were well-correlated with starch accumulation in the seeds. This study suggests that the starch biosynthesis pathway in Chinese chestnut is similar to that of potato tuber/Arabidopsis leaf and differs from that of maize endosperm. The information provides valuable metabolite and genetic resources for future research in starch and sucrose metabolism in Chinese chestnut tree. C1 [Zhang, Lin; Lin, Qing; Fan, Xiaoming; Zou, Feng; Yuan, De-Yi] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China. [Feng, Yanzhi] China Paulownia Res Ctr, Zhengzhou 450003, Henan Province, Peoples R China. [Zeng, Xiaochun; Cao, Heping] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Cao, HP (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Heping.Cao@ars.usda.gov FU Chinese National Science and Technology Pillar Program [2013BAD14B04]; USDA-ARS Quality and Utilization of Agricultural Products Research Program 306 through CRIS [6435-41000-102-00D] FX This work was supported by the Chinese National Science and Technology Pillar Program (2013BAD14B04) and USDA-ARS Quality and Utilization of Agricultural Products Research Program 306 through CRIS 6435-41000-102-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 49 TC 2 Z9 2 U1 3 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 28 PY 2015 VL 63 IS 3 BP 929 EP 942 DI 10.1021/jf505247d PG 14 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA CA1SU UT WOS:000348692600023 PM 25537355 ER PT J AU Liu, XM Zhang, SZ Whitworth, RJ Stuart, JJ Chen, MS AF Liu, Xuming Zhang, Shize Whitworth, R. Jeff Stuart, Jeffrey J. Chen, Ming-Shun TI Unbalanced Activation of Glutathione Metabolic Pathways Suggests Potential Involvement in Plant Defense against the Gall Midge Mayetiola destructor in Wheat SO SCIENTIFIC REPORTS LA English DT Article ID APOPLASTIC OXIDATIVE BURST; HESSIAN FLY DIPTERA; GENE-EXPRESSION; HYPERSENSITIVE RESPONSE; DISEASE RESISTANCE; S-TRANSFERASES; ARABIDOPSIS; LARVAE; CELLS; SYNTHETASE AB Glutathione, gamma-glutamylcysteinylglycine, exists abundantly in nearly all organisms. Glutathione participates in various physiological processes involved in redox reactions by serving as an electron donor/acceptor. We found that the abundance of total glutathione increased up to 60% in resistant wheat plants within 72 hours following attack by the gall midge Mayetiola destructor, the Hessian fly. The increase in total glutathione abundance, however, is coupled with an unbalanced activation of glutathione metabolic pathways. The activity and transcript abundance of glutathione peroxidases, which convert reduced glutathione (GSH) to oxidized glutathione (GSSG), increased in infested resistant plants. However, the enzymatic activity and transcript abundance of glutathione reductases, which convert GSSG back to GSH, did not change. This unbalanced regulation of the glutathione oxidation/reduction cycle indicates the existence of an alternative pathway to regenerate GSH from GSSG to maintain a stable GSSG/GSH ratio. Our data suggest the possibility that GSSG is transported from cytosol to apoplast to serve as an oxidant for class III peroxidases to generate reactive oxygen species for plant defense against Hessian fly larvae. Our results provide a foundation for elucidating the molecular processes involved in glutathione-mediated plant resistance to Hessian fly and potentially other pests as well. C1 [Liu, Xuming; Whitworth, R. Jeff; Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Zhang, Shize] Northwest A&F Univ, Coll Plant Protect, Dept Entomol, Yangling 712100, Shaanxi, Peoples R China. [Stuart, Jeffrey J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Chen, MS (reprint author), Kansas State Univ, Dept Entomol, 123 Waters Hall, Manhattan, KS 66506 USA. EM mchen@ksu.edu FU U.S. Department of Agriculture (USDA) [NIFA 2010-03741] FX This paper is a joint contribution from the United States Department of Agriculture-Agriculture Research Service at Manhattan, Kansas, and the Kansas Agricultural Experiment Station. We thank Dr. Subbaratnam Muthukrishnan for reviewing an early version of the manuscript. Mention of a commercial or proprietary product does not constitute an endorsement or recommendation for its use by the USDA. The research was partially supported by a grant from the U.S. Department of Agriculture (USDA NIFA 2010-03741). NR 43 TC 4 Z9 4 U1 2 U2 21 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JAN 28 PY 2015 VL 5 AR 8092 DI 10.1038/srep08092 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AZ8AG UT WOS:000348436500006 PM 25627558 ER PT J AU Yeroslavsky, G Girshevitz, O Foster-Frey, J Donovan, DM Rahimipour, S AF Yeroslavsky, Gil Girshevitz, Olga Foster-Frey, Juli Donovan, David M. Rahimipour, Shai TI Antibacterial and Antibiofilm Surfaces through Polydopamine-Assisted Immobilization of Lysostaphin as an Antibacterial Enzyme SO LANGMUIR LA English DT Article ID TRANSFER RADICAL POLYMERIZATION; ANTIMICROBIAL ACTIVITY; STAPHYLOCOCCUS-AUREUS; BACTERIOLYTIC AGENT; RESISTANCE; COATINGS; POLYPROPYLENE; ENDOLYSINS; LYSOZYME; BIOFILMS AB Antibiotic resistance and the colonization of bacteria on surfaces, often as biofilms, prolong hospitalization periods, increase mortality, and are thus major concerns for health care providers. There is an urgent need for antimicrobial and antibiofilm surface treatments that are permanent, can eradicate both biofilms and planktonic pathogens over long periods of time, and do not select for resistant strains. In this study, we have demonstrated a simple, robust, and biocompatible method that utilizes the adhesive property of polydopamine (PDA) to covalently attach the antimicrobial enzyme lysostaphin (Lst) to a variety of surfaces to generate antibacterial and antibiofilm interfaces. The immobilization of the recombinant Lst onto PDA-coated surfaces was carried out under physiological conditions, most probably through the C-terminal His6-tag fragment of the enzyme, minimizing the losses of bioagent activity. The modified surfaces were extensively characterized by X-ray photoelectron spectroscopy and peak force quantitative nanomechanical mapping (PeakForce QNM) AFM-based method, and the presence of Lst on the surfaces was further confirmed immunochemically using anti-Lst antibody. We also found that, in contrast to the physically adsorbed Lst, the covalently attached Lst does not leach from the surfaces and maintains its endopeptidase activity to degrade the staphylococcal cell wall, avoiding most intracellular bacterial resistance mechanisms. Moreover, the Lst-coated surfaces kill hospital strains of Staphylococcus aureus in less than 15 min and prevent biofilm formation. This immobilization method should be applicable also to other proteins and enzymes that are recombinantly expressed to include the His6-tag fragment. C1 [Yeroslavsky, Gil; Girshevitz, Olga; Rahimipour, Shai] Bar Ilan Univ, Dept Chem, IL-5290002 Ramat Gan, Israel. [Girshevitz, Olga] Bar Ilan Univ, Inst Nanotechnol & Adv Mat, IL-5290002 Ramat Gan, Israel. [Foster-Frey, Juli; Donovan, David M.] ARS, ABBL, BARC, USDA, Beltsville, MD 20705 USA. RP Donovan, DM (reprint author), ARS, ABBL, BARC, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM David.Donovan@ARS.USDA.GOV; rahimis@biu.ac.il FU BARD, The United States-Israel Binational Agricultural Research and Development Fund [IS-4573-12 R] FX This research was supported by Research Grant IS-4573-12 R from BARD, The United States-Israel Binational Agricultural Research and Development Fund. The authors also acknowledge that mentioning of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. The USDA is an equal opportunity provider and employer. We would like to acknowledge Dr. Yosef Gofer (Department of Chemistry, Bar-Ilan University) and Dr. Hagai Cohen (Department of Chemical Research Support, Weizmann Institute of Science) for the XPS analysis. NR 55 TC 16 Z9 16 U1 15 U2 116 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0743-7463 J9 LANGMUIR JI Langmuir PD JAN 27 PY 2015 VL 31 IS 3 BP 1064 EP 1073 DI 10.1021/la503911m PG 10 WC Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary SC Chemistry; Materials Science GA CA1RQ UT WOS:000348689700023 PM 25547537 ER PT J AU Mayagaya, VS Ntamatungiro, AJ Moore, SJ Wirtz, RA Dowell, FE Maia, MF AF Mayagaya, Valeriana Simon Ntamatungiro, Alex John Moore, Sarah Jane Wirtz, Robert Andrew Dowell, Floyd Ercell Maia, Marta Ferreira TI Evaluating preservation methods for identifying Anopheles gambiae s.s. and Anopheles arabiensis complex mosquitoes species using near infra-red spectroscopy SO PARASITES & VECTORS LA English DT Article DE Near-infrared spectroscopy; Anopheles gambiae s.s; Anopheles arabiensis; Species identification; Preservation methods; Malaria ID INOCULATION RATES; MALARIA CONTROL; AGE; BEHAVIOR; AFRICA; TRANSMISSION; MORTALITY; NETS AB Background: Near-infrared spectroscopy (NIRS) has been successfully used on fresh and RNAlater (R)-preserved members of the Anopheles gambiae complex to identify sibling species and age. No preservation methods other than using RNAlater (R) have been tested to preserve mosquitoes for species identification using NIRS. However, RNAlater (R) is not the most practical preservative for field settings because it is expensive, requires basic laboratory conditions for storage and is not widely available in sub-Saharan Africa. The aim of this study was to test several cheaper and more field-friendly preservation methods for identifying sibling species of the An. gambiae complex using NIRS. Methods: In this study we describe the use of NIRS to identify sibling species of preserved An. gambiae s.s. and An. arabiensis. Mosquitoes of each species were placed in sample tubes and preserved using one of the following preservation methods: (i) refrigeration at 4 degrees C, (ii) freezing at -20 degrees C, (iii) drying over a silica-gel desiccant, (iv) submersion in RNAlater (R) at room temperature, (v) submersion in RNAlater (R) at 4 degrees C, and (vi) submersion in RNAlater (R) at -20 degrees C. Mosquitoes were preserved for 1, 4, 10, 32 or 50 weeks before they were scanned. Results: Storage at 4 degrees C was the only preservation method that, up to 32 weeks, did not result in significantly lower predicted values than those obtained from fresh insects. After 50 weeks, however, refrigerated samples did not give meaningful results. When storing for 50 weeks, desiccating samples over silica gel was the best preservation method, with a partial least squares regression cross-validation of >80%. Predictive data values were analyzed using a generalized linear model. Conclusion: NIRS can be used to identify species of desiccated Anopheles gambiae s.s. and Anopheles arabiensis for up to 50 weeks of storage with more than 80% accuracy. C1 [Mayagaya, Valeriana Simon; Ntamatungiro, Alex John; Moore, Sarah Jane; Maia, Marta Ferreira] Ifakara Hlth Inst, Ifakara, Tanzania. [Ntamatungiro, Alex John] Univ London London Sch Hyg & Trop Med, London WC1E 7HT, England. [Moore, Sarah Jane; Maia, Marta Ferreira] Swiss Trop & Publ Hlth Inst, CH-4002 Basel, Switzerland. [Moore, Sarah Jane; Maia, Marta Ferreira] Univ Basel, CH-4003 Basel, Switzerland. [Wirtz, Robert Andrew] Ctr Dis Control & Prevent, Atlanta, GA 30329 USA. [Dowell, Floyd Ercell] USDA ARS, Ctr Grain & Anim Hlth Res, Engn & Wind Eros Res Unit, Manhattan, KS USA. RP Mayagaya, VS (reprint author), Ifakara Hlth Inst, POB 53, Ifakara, Tanzania. EM vmayagaya@ihi.or.tz OI Ferreira Maia, Marta/0000-0003-3302-5610 FU Centers for Disease Control and Prevention (CDC) FX Our gratitude goes to Ally Daraja, Paulina Kasanga, Neema Nombo and Zuhura Kondo for rearing the mosquitoes we used in this study. This study was funded by the Centers for Disease Control and Prevention (CDC). NR 15 TC 4 Z9 4 U1 1 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD JAN 27 PY 2015 VL 8 AR 60 DI 10.1186/s13071-015-0661-4 PG 6 WC Parasitology SC Parasitology GA CB1XH UT WOS:000349420800001 PM 25623484 ER PT J AU Liu, K Lyu, L Chin, D Gao, JY Sun, XR Shang, F Caceres, A Chang, ML Rowan, S Peng, JM Mathias, R Kasahara, H Jiang, SH Taylor, A AF Liu, Ke Lyu, Lei Chin, David Gao, Junyuan Sun, Xiurong Shang, Fu Caceres, Andrea Chang, Min-Lee Rowan, Sheldon Peng, Junmin Mathias, Richard Kasahara, Hideko Jiang, Shuhong Taylor, Allen TI Altered ubiquitin causes perturbed calcium homeostasis, hyperactivation of calpain, dysregulated differentiation, and cataract SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE cataract; ubiqutin; calpain; connexin; development ID LENS DEVELOPMENT; GAP-JUNCTIONS; PROTEASOME; CONNEXINS; DEGRADATION; PROTEOLYSIS; EXPRESSION; PATHWAY; DISEASE; GROWTH AB Although the ocular lens shares many features with other tissues, it is unique in that it retains its cells throughout life, making it ideal for studies of differentiation/development. Precipitation of proteins results in lens opacification, or cataract, the major blinding disease. Lysines on ubiquitin (Ub) determine fates of Ub-protein substrates. Information regarding ubiquitin proteasome systems (UPSs), specifically of K6 in ubiquitin, is undeveloped. We expressed in the lens a mutant Ub containing a K6W substitution (K6W-Ub). Protein profiles of lenses that express wild-type ubiquitin (WT-Ub) or K6W-Ub differ by only similar to 2%. Despite these quantitatively minor differences, in K6W-Ub lenses and multiple model systems we observed a fourfold Ca2+ elevation and hyperactivation of calpain in the core of the lens, as well as calpain-associated fragmentation of critical lens proteins including Filensin, Fodrin, Vimentin, beta-Crystallin, Caprin family member 2, and tudor domain containing 7. Truncations can be cataractogenic. Additionally, we observed accumulation of gap junction Connexin43, and diminished Connexin46 levels in vivo and in vitro. These findings suggest that mutation of Ub K6 alters UPS function, perturbs gap junction function, resulting in Ca2+ elevation, hyperactivation of calpain, and associated cleavage of substrates, culminating in developmental defects and a cataractous lens. The data show previously unidentified connections between UPS and calpain-based degradative systems and advance our understanding of roles for Ub K6 in eye development. They also inform about new approaches to delay cataract and other protein precipitation diseases. C1 [Liu, Ke; Lyu, Lei; Shang, Fu; Caceres, Andrea; Chang, Min-Lee; Rowan, Sheldon; Jiang, Shuhong; Taylor, Allen] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. [Liu, Ke; Lyu, Lei] Sichuan Univ, Coll Life Sci, Key Lab Bioresources & Ecoenvironm, Minist Educ, Chengdu 610064, Sichuan, Peoples R China. [Chin, David; Peng, Junmin] St Jude Childrens Res Hosp, St Jude Prote Facil, Dept Struct Biol, Memphis, TN 38105 USA. [Chin, David; Peng, Junmin] St Jude Childrens Res Hosp, St Jude Prote Facil, Dept Dev Neurobiol, Memphis, TN 38105 USA. [Gao, Junyuan; Sun, Xiurong; Mathias, Richard] SUNY Stony Brook, Dept Physiol & Biophys, Stony Brook, NY 11794 USA. [Kasahara, Hideko] Univ Florida, Coll Med, Gainesville, FL 32610 USA. [Taylor, Allen] Weizmann Inst Sci, Dept Biol Chem, IL-7610001 Rehovot, Israel. RP Liu, K (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Lab Nutr & Vis Res, Boston, MA 02111 USA. EM kliu@scu.edu.cn; allen.taylor@tufts.edu FU US Department of Agriculture [1950-510000-060-01A]; NIH [R01EY13250, R01EY21212, R01EY06391, R21NS081571]; Alcon gift; American Lebanese Syrian Associated Charities FX We thank Dr. Shinichiro Chuma (Kyoto University), Dr. Christina E. Loren (University of British Columbia), Dr. Lena Gunhaga (Umea University), and Dr. Paul FitzGerald (University of California, Davis) for gifting antibodies; Drs. Murry and Reinecke for Cx43 virus; and Drs. Vierstra and Kim for gifting GST-TUBE plasmid. We appreciate comments from Dr. Elizabeth Whitcomb and Andrew Kovalenko in review of this manuscript. This work was supported by US Department of Agriculture Grant 1950-510000-060-01A (to A.T.), NIH Grants R01EY13250 (to A.T.), R01EY21212 (to A.T.), R01EY06391 (to R.M.), and R21NS081571 (to J.P.), Alcon gift (to A.T.), and American Lebanese Syrian Associated Charities (J.P.). NR 46 TC 7 Z9 9 U1 1 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 27 PY 2015 VL 112 IS 4 BP 1071 EP 1076 DI 10.1073/pnas.1404059112 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AZ7SB UT WOS:000348417000041 PM 25583491 ER PT J AU Darwish, O Shahan, R Liu, ZC Slovin, JP Alkharouf, NW AF Darwish, Omar Shahan, Rachel Liu, Zhongchi Slovin, Janet P. Alkharouf, Nadim W. TI Re-annotation of the woodland strawberry (Fragaria vesca) genome SO BMC GENOMICS LA English DT Article DE Annotation; Strawberry; Fragaria vesca; Transcriptome; Genome; RNA-Seq; Gene; Rosaceae ID HIDDEN MARKOV MODEL; RNA-SEQ; GENE PREDICTION; ALIGNMENT; IDENTIFICATION; TRANSCRIPTS; SEQUENCES; BLAST2GO; TOPHAT; TOOL AB Background: Fragaria vesca is a low-growing, small-fruited diploid strawberry species commonly called woodland strawberry. It is native to temperate regions of Eurasia and North America and while it produces edible fruits, it is most highly useful as an experimental perennial plant system that can serve as a model for the agriculturally important Rosaceae family. A draft of the F. vesca genome sequence was published in 2011 [Nat Genet 43:223,2011]. The first generation annotation (version 1.1) were developed using GeneMark-ES+[Nuc Acids Res 33:6494,2005] which is a self-training gene prediction tool that relies primarily on the combination of ab initio predictions with mapping high confidence ESTs in addition to mapping gene deserts from transposable elements. Based on over 25 different tissue transcriptomes, we have revised the F. vesca genome annotation, thereby providing several improvements over version 1.1. Results: The new annotation, which was achieved using Maker, describes many more predicted protein coding genes compared to the GeneMark generated annotation that is currently hosted at the Genome Database for Rosaceae (http://www.rosaceae.org/). Our new annotation also results in an increase in the overall total coding length, and the number of coding regions found. The total number of gene predictions that do not overlap with the previous annotations is 2286, most of which were found to be homologous to other plant genes. We have experimentally verified one of the new gene model predictions to validate our results. Conclusions: Using the RNA-Seq transcriptome sequences from 25 diverse tissue types, the re-annotation pipeline improved existing annotations by increasing the annotation accuracy based on extensive transcriptome data. It uncovered new genes, added exons to current genes, and extended or merged exons. This complete genome re-annotation will significantly benefit functional genomic studies of the strawberry and other members of the Rosaceae. C1 [Darwish, Omar; Alkharouf, Nadim W.] Towson Univ, Dept Comp & Informat Sci, Towson, MD 21252 USA. [Shahan, Rachel; Liu, Zhongchi] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. [Slovin, Janet P.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. RP Alkharouf, NW (reprint author), Towson Univ, Dept Comp & Informat Sci, 7800 York Rd, Towson, MD 21252 USA. EM NALKHAROUF@TOWSON.EDU FU National Science Foundation [MCB0923913] FX This work was supported by National Science Foundation Grant MCB0923913 to Z.L. J.S and N.A. All bioinformatics pipelines were executed on iPlant Atmosphere cloud service. NR 25 TC 8 Z9 9 U1 5 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JAN 27 PY 2015 VL 16 AR 29 DI 10.1186/s12864-015-1221-1 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CA5GP UT WOS:000348937200001 PM 25623424 ER PT J AU Li, RG Baysal-Gurel, F Abdo, Z Miller, SA Ling, KS AF Li, Rugang Baysal-Gurel, Fulya Abdo, Zaid Miller, Sally A. Ling, Kai-Shu TI Evaluation of disinfectants to prevent mechanical transmission of viruses and a viroid in greenhouse tomato production SO Virology Journal LA English DT Article DE Pepino mosaic virus; Tomato mosaic virus; Tobacco mosaic virus; Potato spindle tuber viroid; Disinfectant; Mechanical transmission; Greenhouse tomato ID PEPINO-MOSAIC-VIRUS; RT-PCR ASSAY; 1ST REPORT; INFECTING TOMATO; NATURAL OCCURRENCE; GENOMIC RNA; SEQUENCE; ISOLATE; INACTIVATION; DISTINCT AB Background: In recent years, a number of serious disease outbreaks caused by viruses and viroids on greenhouse tomatoes in North America have resulted in significant economic losses to growers. The objectives of this study were to evaluate the effectiveness of commercial disinfectants against mechanical transmission of these pathogens, and to select disinfectants with broad spectrum reactivity to control general virus and viroid diseases in greenhouse tomato production. Methods: A total of 16 disinfectants were evaluated against Pepino mosaic virus (PepMV), Potato spindle tuber viroid (PSTVd), Tomato mosaic virus (ToMV), and Tobacco mosaic virus (TMV). The efficacy of each disinfectant to deactivate the pathogen's infectivity was evaluated in replicate experiments from at least three independent experiments. Any infectivity that remained in the treated solutions was assessed through bioassays on susceptible tomato plants through mechanical inoculation using inocula that had been exposed with the individual disinfectant for three short time periods (0-10 sec, 30 sec and 60 sec). A positive infection on the inoculated plant was determined through symptom observation and confirmed with enzyme-linked immunosorbent assay (PepMV, ToMV, and TMV) and real-time reverse transcription-PCR (PSTVd). Experimental data were analyzed using Logistic regression and the Bayesian methodology. Results: Statistical analyses using logistic regression and the Bayesian methodology indicated that two disinfectants (2% Virkon S and 10% Clorox regular bleach) were the most effective to prevent transmission of PepMV, PSTVd, ToMV, and TMV from mechanical inoculation. Lysol all-purpose cleaner (50%) and nonfat dry milk (20%) were also effective against ToMV and TMV, but with only partial effects for PepMV and PSTVd. Conclusion: With the broad spectrum efficacy against three common viruses and a viroid, several disinfectants, including 2% Virkon S, 10% Clorox regular bleach and 20% nonfat dry milk, are recommend to greenhouse facilities for consideration to prevent general virus and viroid infection on tomato plants. C1 [Li, Rugang; Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. [Baysal-Gurel, Fulya; Miller, Sally A.] Ohio State Univ, OARDC, Dept Plant Pathol, Wooster, OH 44691 USA. [Abdo, Zaid] USDA ARS, South Atlantic Area, Athens, GA 30605 USA. RP Ling, KS (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM kai.ling@ars.usda.gov RI Miller, Sally/A-1240-2015; Abdo, Zaid/E-5176-2017 OI Miller, Sally/0000-0001-9611-0535; Abdo, Zaid/0000-0002-8272-7734 FU USDA, National Institute of Food and Agriculture, Specialty Crop Research Initiative Program [SCRI-2010-600-25320] FX We thank Chester Kurowski of Monsanto Company for valuable discussions, and Andrea Gilliard, Alan Wilder and Mindie Lipphardt who provided excellent technical assistance. Chellappan Padmanabhan's review comments are greatly appreciated. Several companies provided products at no charge: BASF, BioSafe Systems LLC, Des-O-Germ (PTy) LTD, Ecolab Center, MENNO CHEMIE-VERTRIEB GMBH, Enviro Tech Chemical Services, Inc., Germ-Free Planet, and PACE 49 Inc. This work is supported in-part by a grant from the USDA, National Institute of Food and Agriculture, Specialty Crop Research Initiative Program (SCRI-2010-600-25320) to SAM and KSL. NR 45 TC 1 Z9 1 U1 6 U2 39 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-422X J9 VIROL J JI Virol. J. PD JAN 27 PY 2015 VL 12 AR 5 DI 10.1186/s12985-014-0237-5 PG 11 WC Virology SC Virology GA CA5HK UT WOS:000348939300002 PM 25623384 ER PT J AU Riggs, RA Keane, RE Cimon, N Cook, R Holsinger, L Cook, J DelCurto, T Baggett, LS Justice, D Powell, D Vavra, M Naylor, B AF Riggs, Robert A. Keane, Robert E. Cimon, Norm Cook, Rachel Holsinger, Lisa Cook, John DelCurto, Timothy Baggett, L. Scott Justice, Donald Powell, David Vavra, Martin Naylor, Bridgett TI Biomass and fire dynamics in a temperate forest-grassland mosaic: Integrating multi-species herbivory, climate, and fire with the FireBGCv2/GrazeBGC system SO ECOLOGICAL MODELLING LA English DT Article DE Herbivory; Fire; Climate; Succession; Modeling; FireBGCv2 ID NATIONAL-PARK; ECOSYSTEM PROCESSES; HABITAT SELECTION; MULE DEER; REGIONAL APPLICATIONS; LOCALIZED MANAGEMENT; NORTHEASTERN OREGON; FUNCTIONAL-RESPONSE; VEGETATION DYNAMICS; BOREAL FORESTS AB Landscape fire succession models (LFSMs) predict spatially explicit interactions between vegetation succession and disturbance, but these models have yet to fully integrate ungulate herbivory as a driver of their processes. We modified a complex LFSM, FireBGCv2, to include a multi-species herbivory module, GrazeBGC. The system is novel in that it explicitly accommodates multiple herbivore populations, inter-and intra-specific spatial forcing of their forage demands, and site-specific dietary selectivity to interactively modify biomass, fuels and fire behavior across a landscape and over time. A factorial experiment with five grazing regimes, three climates and two fire-management scenarios generated interactive influences on undergrowth biomass (shrub, herb, total), surface-fire (fire-line intensity; flame length; scorch height; soil heat; CO, CO2, CH4, and PM2.5 emissions), and the landscape's fire-return interval. Herbivory's effects increased with biophysical site potential and herbivore forage demand, but its effects were also contingent on climate and fire-suppression. Multi-species grazing modified biomass and fire within stands and biophysical sites, but regimes involving only wildlife or livestock were less effectual. Multi-species herbivory affected the landscape's fire-return interval, but otherwise it did not "scale up" to significantly modify total landscape respiration, primary production, carbon, or the total area burned by individual fires. As modeled here, climate change and the effectiveness of future fire suppression exerted stronger effects on landscape metabolism and carbon than did herbivory. (C) 2014 The Authors. Published by Elsevier B.V. C1 [Keane, Robert E.; Holsinger, Lisa] US Forest Serv, Missoula Fire Sci Lab, Mt Res Stn, Missoula, MT 59808 USA. [Cimon, Norm; Vavra, Martin; Naylor, Bridgett] Info Synchron LLC, La Grande, OR 97850 USA. [Cook, Rachel; Cook, John] Natl Council Air & Stream Improvement, La Grande, OR 97850 USA. [DelCurto, Timothy] Oregon State Univ, Eastern Oregon Agr Res Ctr, Union, OR 97883 USA. [Baggett, L. Scott] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Justice, Donald; Powell, David] Umatilla Natl Forest, Pendleton, OR 97801 USA. RP Riggs, RA (reprint author), 62710 Booth Lane, La Grande, OR 97850 USA. EM drydog@oregonwireless.net FU "New Fire Science Initiative" grant from the United States Joint Fire Science Program (JFSP) [09-3-01-20] FX This work was supported by a "New Fire Science Initiative" grant from the United States Joint Fire Science Program (JFSP No.: 09-3-01-20). We are grateful to Craig Busskohl (Umatilla National Forest) and Dave Trochlell (Natural Resources Conservation Service) for help interpreting soil surveys. Rachel Loehman and Jason Clark (USFS Missoula Fire Science Lab) helped the senior author learn the simulation platform. Brian Dick (Starkey Experimental Forest and Range) provided crucial administrative support. Michael Hansen (Oregon Department of Fish and Wildlife), Larry Irwin and Jake Verschuyl (National Council for Air and Stream Improvement), Brian Kernohan (Hancock Timber Resource Group), Richard Senft (Amber Waves Software) and two anonymous reviewers offered thoughtful and constructive suggestions that helped us improve the manuscript. NR 160 TC 2 Z9 2 U1 7 U2 63 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD JAN 24 PY 2015 VL 296 BP 57 EP 78 DI 10.1016/j.ecolmodel.2014.10.013 PG 22 WC Ecology SC Environmental Sciences & Ecology GA AX5FX UT WOS:000346953200006 ER PT J AU Coulston, JW Wear, DN Vose, JM AF Coulston, John W. Wear, David N. Vose, James M. TI Complex forest dynamics indicate potential for slowing carbon accumulation in the southeastern United States SO SCIENTIFIC REPORTS LA English DT Article ID LAND-USE; SEQUESTRATION; RESPONSES; IMPACTS; DROUGHT; GROWTH; SINK AB Over the past century forest regrowth in Europe and North America expanded forest carbon (C) sinks and offset C emissions but future C accumulation is uncertain. Policy makers need insights into forest C dynamics as they anticipate emissions futures and goals. We used land use and forest inventory data to estimate how forest C dynamics have changed in the southeastern United States and attribute changes to land use, management, and disturbance causes. From 2007-2012, forests yielded a net sink of C because of net land use change (16.48 Tg C yr(-1)) and net biomass accumulation (+75.4 Tg C yr(-1)). Forests disturbed by weather, insect/disease, and fire show dampened yet positive forest C changes (+1.56, +1.4, +5.48 Tg C yr(-1), respectively). Forest cutting caused net decreases in C(-76.7 TgC yr(-1)) but was offset by forest growth (+143.77 Tg C yr(-1)). Forest growth rates depend on age or stage of development and projected C stock changes indicate a gradual slowing of carbon accumulation with anticipated forest aging (a reduction of 9.5% over the next five years). Additionally, small shifts in land use transitions consistent with economic futures resulted in a 40.6% decrease in C accumulation. C1 [Coulston, John W.] US Forest Serv, USDA, Knoxville, TN 37919 USA. [Wear, David N.; Vose, James M.] N Carolina State Univ, US Forest Serv, USDA, Raleigh, NC 27695 USA. RP Coulston, JW (reprint author), US Forest Serv, USDA, 4700 Old Kingston Pike, Knoxville, TN 37919 USA. EM jcoulston@fs.fed.us NR 31 TC 7 Z9 7 U1 0 U2 19 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JAN 23 PY 2015 VL 5 AR 8002 DI 10.1038/srep08002 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AZ5WA UT WOS:000348287600004 PM 25614123 ER PT J AU Tako, E Reed, SM Budiman, J Hart, JJ Glahn, RP AF Tako, Elad Reed, Spenser M. Budiman, Jessica Hart, Jonathan J. Glahn, Raymond P. TI Higher iron pearl millet (Pennisetum glaucum L.) provides more absorbable iron that is limited by increased polyphenolic content SO NUTRITION JOURNAL LA English DT Article DE Pearl millet; Biofortification; Iron bioavailability; Polyphenols; In vitro digestion/Caco-2 cell model; Broiler chicken ID PHASEOLUS-VULGARIS L.; BEAN DIET PROVIDE; GALLUS-GALLUS; BIOAVAILABLE IRON; COMPOSITE MEALS; ABSORPTION; BIOFORTIFICATION; MAIZE; MODEL; ZINC AB Background: Our objective was to compare the capacity of iron (Fe) biofortified and standard pearl millet (Pennisetum glaucum L.) to deliver Fe for hemoglobin (Hb)-synthesis. Pearl millet (PM) is common in West-Africa and India, and is well adapted to growing areas characterized by drought, low-soil fertility, and high-temperature. Because of its tolerance to difficult growing conditions, it can be grown in areas where other cereal crops, such as maize, would not survive. It accounts for approximately 50% of the total world-production of millet. Given the widespread use of PM in areas of the world affected by Fe-deficiency, it is important to establish whether biofortified-PM can improve Fe-nutriture. Methods: Two isolines of PM, a low-Fe-control ("DG-9444", Low-Fe) and biofortified ("ICTP-8203 Fe", High-Fe) in Fe (26 mu g and 85 mu g-Fe/g, respectively) were used. PM-based diets were formulated to meet the nutrient requirements for the broiler (Gallus-gallus) except for Fe (Fe concentrations were 22.1 +/- 0.52 and 78.6 +/- 0.51 mu g-Fe/g for the Low-Fe and High-Fe diets, respectively). For 6-weeks, Hb, feed-consumption and body-weight were measured (n = 12). Results: Improved Fe-status was observed in the High-Fe group, as suggested by total-Hb-Fe values (15.5 +/- 0.8 and 26.7 +/- 1.4 mg, Low-Fe and High-Fe respectively, P<0.05). DMT-1, DcytB, and ferroportin mRNA-expression was higher (P<0.05) and liver-ferritin was lower (P>0.05) in the Low-Fe group versus High-Fe group. In-vitro comparisons indicated that the High-Fe PM should provide more absorbable-Fe; however, the cell-ferritin values of the in-vitro bioassay were very low. Such low in-vitro values, and as previously demonstrated, indicate the presence of high-levels of polyphenolic-compounds or/and phytic-acid that inhibit Fe-absorption. LC/MS-analysis yielded 15 unique parent aglycone polyphenolic-compounds elevated in the High-Fe line, corresponding to m/z = 431.09. Conclusions: The High-Fe diet appeared to deliver more absorbable-Fe as evidenced by the increased Hb and Hb-Fe status. Results suggest that some PM varieties with higher Fe contents also contain elevated polyphenolic concentrations, which inhibit Fe-bioavailability. Our observations are important as these polyphenols-compounds represent potential targets which can perhaps be manipulated during the breeding process to yield improved dietary Fe-bioavailability. Therefore, the polyphenolic and phytate profiles of PM must be carefully evaluated in order to further improve the nutritional benefit of this crop. C1 [Tako, Elad; Reed, Spenser M.; Hart, Jonathan J.; Glahn, Raymond P.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Reed, Spenser M.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Budiman, Jessica] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. RP Tako, E (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM et79@cornell.edu NR 45 TC 10 Z9 10 U1 5 U2 28 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1475-2891 J9 NUTR J JI Nutr. J. PD JAN 23 PY 2015 VL 14 AR 11 DI 10.1186/1475-2891-14-11 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA CA3OR UT WOS:000348816900001 PM 25614193 ER PT J AU Andrade, NA Lozano, N McConnell, LL Torrents, A Rice, CP Ramirez, M AF Andrade, Natasha A. Lozano, Nuria McConnell, Laura L. Torrents, Alba Rice, Clifford P. Ramirez, Mark TI Long-term trends of PBDEs, triclosan, and triclocarban in biosolids from a wastewater treatment plant in the Mid-Atlantic region of the US SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE Biosolids; Sewage sludge; PBDEs; Triciosan; Triclocarban ID POLYBROMINATED DIPHENYL ETHERS; BROMINATED FLAME RETARDANTS; TANDEM MASS-SPECTROMETRY; SEWAGE-SLUDGE; ORGANIC CONTAMINANTS; LAND APPLICATION; SOIL; ANTIMICROBIALS; MUNICIPAL; EXPOSURE AB In the US, land application of biosolids has been utilized in government-regulated programs to recycle valuable nutrients and organic carbon that would otherwise be incinerated or buried in landfills. While many benefits have been reported, there are concerns that these practices represent a source of organic micropollutants to the environment. In this study, biosolids samples from a wastewater treatment plant in the Mid-Atlantic region of the US were collected approximately every 2 months over a 7-year period and analyzed for brominated diphenyl ethers (BDE-47, BDE-99, and BDE-209), triclosan, and triclocarban. During the collection 'period of 2005-2011, concentrations of the brominated diphenyl ethers BDE-47 + BDE-99 decreased by 42%, triclocarban decreased by 47%, but BDE-209 and triclosan remained fairly constant. Observed reductions in contaminant concentrations could not be explained by different seasons or by volumetric changes of wastewaters arriving at the treatment plant and instead may be the result of the recent phaseout of BDE-47 and BDE-99 as well as potential reductions in the use of triclocarban. (C) 2014 Elsevier B.V. All rights reserved. C1 [Andrade, Natasha A.; Lozano, Nuria; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [McConnell, Laura L.; Rice, Clifford P.] USDA ARS, Beltsville Agr Res Ctr, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. [Ramirez, Mark] Dist Columbia Water & Sewer Author, DC Water, Washington, DC 20032 USA. RP Andrade, NA (reprint author), Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. EM nandrade@umd.edu FU District of Columbia Water and Sewer Authority, Washington DC; Agricultural Research Service US Department of Agriculture laboratories at Beltsville, MD FX This study was partially supported by the District of Columbia Water and Sewer Authority, Washington DC, and the Agricultural Research Service US Department of Agriculture laboratories at Beltsville, MD. The authors want to acknowledge and thank the reviewers of this article for their thoughtful and thorough review which undoubtedly produced a higher quality manuscript. NR 59 TC 9 Z9 9 U1 3 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD JAN 23 PY 2015 VL 282 SI SI BP 68 EP 74 DI 10.1016/j.jhazmat.2014.09.028 PG 7 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AW3WG UT WOS:000346213400010 PM 25282513 ER PT J AU Natilla, A Murphy, C Hammond, RW AF Natilla, Angela Murphy, Charles Hammond, Rosemarie W. TI Mutations in the alpha-helical region of the amino terminus of the Maize rayado fino virus capsid protein and CP:RNA ratios affect virus-like particle encapsidation of RNAs SO VIRUS RESEARCH LA English DT Article DE Viral-like particle; RNA encapsidation; VLP; Nanoparticle; Protein cage; Maize rayado fino virus ID PHYSALIS MOTTLE VIRUS; CUCUMBER MOSAIC-VIRUS; VIRAL NANOPARTICLES; GENOME; ENCAPSULATION; EXPRESSION; VECTOR; PLANTS; CAGES AB Viral-based nanoplatforms rely on balancing the delicate array of virus properties to optimally achieve encapsidation of foreign materials with various potential objectives. We investigated the use of Maize rayado fino virus (MRFV)-virus-like particles (VLPs) as a multifunctional nanoplatform and their potential application as protein cages. MRFV-VLPs are composed of two serologically related, carboxy co-terminal coat proteins (CP1 and CP2) which are capable of self-assembling in Nicotiana benthamiana plants into 30 nm particles with T=3 symmetry. The N-terminus of CP1 was targeted for genetic modification to exploit the driving forces for VLP assembly, packaging and retention of RNA in vivo and in vitro. The N-terminus of MRFV-CP1 contains a peptide sequence of 37 amino acids which has been predicted to have an alpha-helical structure, is rich in hydrophobic amino acids, facilitates CP-RNA interactions, and is not required for self-assembly. Amino acid substitutions were introduced in the 37 amino acid N-terminus by site-directed mutagenesis and the mutant VLPs produced in plants by a Potato virus X (PVX)-based vector were tested for particle stability and RNA encapsidation. All mutant CPs resulted in production of VLPs which encapsidated non-viral RNAs, including PVX genomic and subgenomic (sg) RNAs, 18S rRNA and cellular and viral mRNAs. In addition, MRFV-VLPs encapsidated GFP mRNA when was expressed in plant cells from the pGD vector. These results suggest that RNA packaging in MRFV-VLPs is predominantly driven by electrostatic interactions between the N-terminal 37 amino acid extension of CP1 and RNA, and that the overall species concentration of RNA in the cellular pool may determine the abundance and species of the RNAs packaged into the VLPs. Furthermore, RNA encapsidation is not required for VLPs stability, VLPs formed from MRFV-CP1 were stable at temperatures up to 70 degrees C, and can be disassembled into CP monomers, which can then reassemble in vitro into complete VLPs either in the absence or presence of RNAs. (C) 2014 Published by Elsevier B.V. C1 [Natilla, Angela; Hammond, Rosemarie W.] Beltsville Agr Res Serv, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Murphy, Charles] Beltsville Agr Res Serv, USDA, Electron & Confocal Microscopy Unit, Beltsville, MD 20705 USA. RP Natilla, A (reprint author), Beltsville Agr Res Serv, USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM natilla.ang@gmail.com NR 49 TC 0 Z9 0 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD JAN 22 PY 2015 VL 196 BP 70 EP 78 DI 10.1016/j.virusres.2014.07.033 PG 9 WC Virology SC Virology GA CB4EV UT WOS:000349581900010 PM 25102332 ER PT J AU Qiu, S Yadav, MP Chen, H Liu, Y Tatsumi, E Yin, LJ AF Qiu, Shuang Yadav, Madhav P. Chen, Hao Liu, Yan Tatsumi, Eizo Yin, Lijun TI Effects of corn fiber gum (CFG) on the pasting and thermal behaviors of maize starch SO CARBOHYDRATE POLYMERS LA English DT Article DE Maize starch; Gelatinization; Corn fiber gum; Leached amylose; Confocal laser scanning microscopy ID WHEAT-STARCH; RHEOLOGICAL PROPERTIES; KONJAC-GLUCOMANNAN; GUAR GUM; RETROGRADATION BEHAVIOR; MOLECULAR-WEIGHTS; POTATO STARCH; RICE STARCH; GELATINIZATION; GELS AB Corn fiber gum (CFG) was a novel arabinoxylan hydrocolloid and recent researches showed its considerable potential in food processing. In this study, the interactions of maize starch and CFG were studied. Maize starch/CFG blend gels were prepared from maize starch suspension mixing with 0.1%, 0.25%, 0.5%, 1.0% (w/w) CFG. The pasting and thermal properties, rheological properties, microstructure, leached amylose and swelling power characteristics were evaluated. Compared with the reference, CFG addition lowered peak viscosity and breakdown of the composite system, but increased final viscosity in RVA measurement. The swelling power and the amount of leached amylose of maize starch gels were reduced as the addition concentration of CFG increased. The thermal characteristics of maize starch/CFG mixtures varied insignificantly as determined in DSC heating process. Rheological parameters, such as storage modulus (G') and loss modulus (G"), of the maize starches were observed to increase when CFG was present, supporting the hypothesis that the interaction between CFG and amylose could happen in the composite system. Confocal laser scanning microscopy (CLSM) confirmed changes in gels microstructure as starch components tended to be inhibited from leaching out of the granules when CFG was added, and the morphology of starch granule was more compact when CFG was added. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Qiu, Shuang; Chen, Hao; Liu, Yan; Yin, Lijun] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China. [Yadav, Madhav P.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Tatsumi, Eizo] Japan Int Res Ctr Agr Sci, Minist Agr Forestry & Fisheries, Tsukuba, Ibaraki 3058686, Japan. [Yin, Lijun] Henan Univ Technol, Zhengzhou 450052, Henan, Peoples R China. RP Yin, LJ (reprint author), POB 40,17, Beijing 100083, Peoples R China. EM ljyin@cau.edu.cn FU National Science Foundation of China [31371719]; National Key-technologies RD Project [2012BAD37B05]; special fund for agro-scientific research in the public interest [201303079]; Chinese Universities Scientific Fund [2013YJ006] FX This research was carried out with financial support from the National Science Foundation of China (No. 31371719), National Key-technologies R&D Project (No. 2012BAD37B05) and special fund for agro-scientific research in the public interest (No. 201303079). The corresponding author also thanks the support from the support from Chinese Universities Scientific Fund (2013YJ006). NR 38 TC 5 Z9 6 U1 4 U2 51 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JAN 22 PY 2015 VL 115 BP 246 EP 252 DI 10.1016/j.carbpol.2014.08.071 PG 7 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA AT3XX UT WOS:000344869800033 PM 25439892 ER PT J AU Sousa, AMM Souza, HKS Uknalis, J Liu, SC Goncalves, MP Liu, LS AF Sousa, Anna M. M. Souza, Hileia K. S. Uknalis, Joseph Liu, Shih-Chuan Goncalves, Maria P. Liu, LinShu TI Electrospinning of agar/PVA aqueous solutions and its relation with rheological properties SO CARBOHYDRATE POLYMERS LA English DT Article DE Agar; PVA; Electrospinning; Tubeless spinneret; Rheology; Nanofibers ID BIOMEDICAL APPLICATIONS; POLYVINYL-ALCOHOL; SODIUM ALGINATE; NANOFIBERS; BIOMATERIALS; EXTRACTION; MORPHOLOGY; VISCOSITY; OXIDE); CHITIN AB In this work, we report the successful fabrication of agar-based nanofibers by electrospinning technique, using water as solvent media. A tubeless spinneret was attached inside the electrospinning chamber, operating at 50 C, to avoid agar gelation. Agar pure solution (1 wt%) showed inadequate spinnability regardless of the used electrospinning conditions. The addition of a co-blending polymer such as PVA (10 wt% starting solution) improved the solutions viscoelasticity and hence, the solutions spinnability. Agar/PVA solutions were prepared with different mass ratios (10010, 50150, 40160, 30170, 20180 and 01100) and electrospun at various sets of electrospinning conditions. Best nanofibers were obtained with 30170 and 20180 agar/PVA blends while samples with higher agar contents (50150 and 40160 agar/PVA) were harder to process and led to discontinuous fibrous mats. This first set of encouraging results can open a new window of opportunities for agar-based biomaterials in the form of nanofibers. Published by Elsevier Ltd. C1 [Sousa, Anna M. M.; Souza, Hileia K. S.; Goncalves, Maria P.] Univ Porto, Fac Engn, REQUIMTE, P-4200465 Oporto, Portugal. [Sousa, Anna M. M.; Uknalis, Joseph; Liu, Shih-Chuan; Liu, LinShu] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Liu, Shih-Chuan] Chung Shan Med Univ, Sch Hlth Diet & Ind Management, Taichung 402, Taiwan. [Liu, Shih-Chuan] Chung Shan Med Univ Hosp, Dept Nutr, Taichung 402, Taiwan. RP Liu, LS (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM LinShu.liu@ars.usda.gov RI Souza, Hileia/D-4739-2013; Goncalves, Maria/D-4641-2013 OI Souza, Hileia/0000-0003-2619-6892; Goncalves, Maria/0000-0002-8873-4097 FU FEDER funds through the Operational Programme for Competitiveness Factors - COMPETE; National Funds through FCT- Foundation for Science and Technology [Pest-C/EQB/LA0006/2013 - FCOMP-01-0124-FEDER-37285]; FCT [SFRH/BD/64315/2009] FX This work is funded by FEDER funds through the Operational Programme for Competitiveness Factors - COMPETE and by National Funds through FCT- Foundation for Science and Technology under the project Pest-C/EQB/LA0006/2013 - FCOMP-01-0124-FEDER-37285. FCT is also truly acknowledged for the concession of A.M.M. Sousa's PhD grant (SFRH/BD/64315/2009). To all financing sources the authors are greatly indebted. NR 37 TC 15 Z9 15 U1 6 U2 95 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JAN 22 PY 2015 VL 115 BP 348 EP 355 DI 10.1016/j.carbpol.2014.08.074 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA AT3XX UT WOS:000344869800045 PM 25439904 ER PT J AU Kaufman, RC Wilson, JD Bean, SR Herald, TJ Shi, YC AF Kaufman, R. C. Wilson, J. D. Bean, S. R. Herald, T. J. Shi, Y. -C. TI Development of a 96-well plate iodine binding assay for amylose content determination SO CARBOHYDRATE POLYMERS LA English DT Article DE Starch; Amylose; Microplate; Cereals ID COLORIMETRIC PROCEDURE; PASTING PROPERTIES; CONCANAVALIN-A; FINE-STRUCTURE; MILLED-RICE; AMYLOPECTIN; STARCH; CHROMATOGRAPHY; CEREAL AB Cereal starch amylose/amylopectin (AM/AP) is critical in functional properties for food and industrial applications. Conventional methods of AM/AP are time consuming and labor intensive making it difficult to screen the large sample sets necessary for evaluating breeding samples and investigating environmental impact on starch development. The objective was to adapt and optimize the iodine binding assay in a 96-well plate format for measurement at both lambda 620 nm and lambda 510 nm. The standard curve for amylose content was scaled to a 96-well plate format and demonstrated R-2 values of 0.999 and 0.993 for single and dual wavelengths, respectively. The plate methods were applicable over large ranges of amylose contents: high amylose maize starch at 61.7 +/- 2.3%, normal wheat starch at 29.0 +/- 0.74%, and a waxy maize starch at 1.2 +/- 0.9%. The method exhibited slightly greater amylose content values than the Concanavalin A method for normal type starches; but is consistent with cuvette scale iodine binding assays. Published by Elsevier Ltd. C1 [Kaufman, R. C.; Wilson, J. D.; Bean, S. R.; Herald, T. J.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Shi, Y. -C.] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. RP Wilson, JD (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM jeff.d.wilson@ars.usda.gov OI Bean, Scott/0000-0001-8678-8094 NR 22 TC 5 Z9 5 U1 14 U2 43 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD JAN 22 PY 2015 VL 115 BP 444 EP 447 DI 10.1016/j.carbpol.2014.09.015 PG 4 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA AT3XX UT WOS:000344869800058 PM 25439917 ER PT J AU Bannantine, JP Talaat, AM AF Bannantine, John P. Talaat, Adel M. TI Controlling Johne's disease: vaccination is the way forward SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE Johne's disease; vaccines; Mycobacterium; paratuberculosis; veterinary medicine ID AVIUM SUBSP PARATUBERCULOSIS; HUMORAL IMMUNE-RESPONSES; LIVE ATTENUATED VACCINE; MYCOBACTERIUM-PARATUBERCULOSIS; GENOME SEQUENCE; DAIRY HERDS; CANDIDATES; CALVES; MUTANTS; IMMUNIZATION C1 [Bannantine, John P.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Talaat, Adel M.] Univ Wisconsin, Dept Pathobiol Sci, Madison, WI 53706 USA. RP Bannantine, JP (reprint author), USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. EM john.bannantine@ars.usda.gov OI Bannantine, John/0000-0002-5692-7898 NR 28 TC 3 Z9 3 U1 0 U2 14 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD JAN 21 PY 2015 VL 5 AR 2 DI 10.3389/fcimb.2015.00002 PG 2 WC Immunology; Microbiology SC Immunology; Microbiology GA CA8HA UT WOS:000349157300001 PM 25653925 ER PT J AU Ashworth, DJ Yates, SR Van Wesenbeeck, IJ Stanghellini, M AF Ashworth, Daniel J. Yates, Scott R. Van Wesenbeeck, Ian J. Stanghellini, Mike TI Effect of Co-formulation of 1,3-Dichloropropene and Chloropicrin on Evaporative Emissions from Soil SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE fumigants; degradation; half-life; soil columns; Telone II; Tri-Clor; Telone C-17; Telone C-35; Pick-Clor 60; Pic-Clor 80 ID METHYL-BROMIDE; FUMIGANTS; DEGRADATION; FIELD; IODIDE; ALTERNATIVES; FUMIGATION AB Co-formulations of 1,3-dichloropropene (1,3-D) and chloropicrin (CP) are commonly used for preplant fumigation in the production of high-value crops. Various ratios of 1,3-D to CP are available in these co-formulations. Collation of previous field data suggested that when the two fumigants were co-applied, the emissions of CP were significantly lower than when CP was applied singly. However, none of these previous studies had a control treatment with CP applied alone, alongside a treatment where CP was co-applied with 1,3-D under the same climatic and edaphic conditions. This work aimed to address this issue by measuring emission fluxes from soil columns maintained under controlled conditions in which 1,3-D and CP were applied alone and as four commercial co-formulations with various 1,3-D:CP ratios. A strong positive relationship between CP emissions and CP percentage in the formulation was observed. Furthermore, strong positive relationships between CP degradation half-life and CP percentage in the formulation and between CP degradation half-life and total column emissions suggested that the lower emissions were due to faster CP degradation when the CP percentage (and hence initial application mass) in the formulation was low. The presence of 1,3-D did not significantly affect the degradation rate of CP, and, therefore, it is hypothesized that co-application was, in itself, not a significant factor in emission losses from the columns. The findings have implications for the accurate modeling of CP because the effect of initial mass applied on CP degradation rate is not usually considered. C1 [Ashworth, Daniel J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Ashworth, Daniel J.; Yates, Scott R.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. [Van Wesenbeeck, Ian J.] Dow AgroSci, Indianapolis, IN 46268 USA. [Stanghellini, Mike] TriCal Inc, Hollister, CA 95024 USA. RP Ashworth, DJ (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. EM Daniel.Ashworth@ars.usda.gov FU TriCal Inc, Hollister, CA FX This work was carried out at the USDA-AIRS U.S. Salinity Laboratory, Riverside, CA, under funding from TriCal Inc, Hollister, CA, the receipt of which is gratefully acknowledged. The statements and conclusions in this report are those of the contractors and not necessarily those of TriCal Inc. The mention of commercial products, their source, or their use in connection with material reported herein is not to be construed as actual or implied endorsement of such products. The use of trade, firm, or corporation names in this document is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 26 TC 4 Z9 4 U1 0 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 21 PY 2015 VL 63 IS 2 BP 415 EP 421 DI 10.1021/jf506010b PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AZ6LZ UT WOS:000348332500006 PM 25531174 ER PT J AU McCormick, SP Kato, T Maragos, CM Busman, M Lattanzio, VMT Galaverna, G Dall-Asta, C Crich, D Price, NPJ Kurtzman, CP AF McCormick, Susan P. Kato, Takayuki Maragos, Chris M. Busman, Mark Lattanzio, Veronica M. T. Galaverna, Gianni Dall-Asta, Chiara Crich, David Price, Neil P. J. Kurtzman, Cletus P. TI Anomericity of T-2 Toxin-glucoside: Masked Mycotoxin in Cereal Crops SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE mycotoxins; trichothecene; masked mycotoxins; ELISA; T-2 toxin; T-2 toxin-glucoside ID TANDEM MASS-SPECTROMETRY; HETEROLOGOUS EXPRESSION; UDP-GLUCOSYLTRANSFERASE; DEOXYNIVALENOL; WHEAT; BARLEY; DIGESTION; MAIZE; GLYCOSYLTRANSFERASES; RESISTANCE AB T-2 toxin is a trichothecene mycotoxin produced when Fusarium fungi infect grains, especially oats and wheat. Ingestion of T-2 toxin contaminated grain can cause diarrhea, hemorrhaging, and feed refusal in livestock. Cereal crops infected with mycotoxin-producing fungi form toxin glycosides, sometimes called masked mycotoxins, which are a potential food safety concern because they are not detectable by standard approaches and may be converted back to the parent toxin during digestion or food processing. The work reported here addresses four aspects of T-2 toxin-glucosides: phytotoxicity, stability after ingestion, antibody detection, and the anomericity of the naturally occurring T-2 toxin-glucoside found in cereal plants. T-2 toxin-beta-glucoside was chemically synthesized and compared to T-2 toxin-a-glucoside prepared with Blastobotrys muscicola cultures and the T-2 toxin-glucoside found in naturally contaminated oats and wheat. The anomeric forms were separated chromatographically and differ in both NMR and mass spectrometry. Both anomers were significantly degraded to T-2 toxin and HT-2 toxin under conditions that mimic human digestion, but with different kinetics and metabolic end products. The naturally occurring T-2 toxin-glucoside from plants was found to be identical to T-2 toxin-alpha a-glucoside prepared with B. muscicola. An antibody test for the detection of T-2 toxin was not effective for the detection of T-2 toxin-a-glucoside. This anomer was produced in sufficient quantity to assess its animal toxicity. C1 [McCormick, Susan P.; Maragos, Chris M.; Busman, Mark; Kurtzman, Cletus P.] USDA, Bacterial Foodbome Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Kato, Takayuki; Crich, David] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA. [Lattanzio, Veronica M. T.] Natl Res Council Italy, Inst Sci Food Prod, I-70126 Bari, Italy. [Galaverna, Gianni; Dall-Asta, Chiara] Univ Parma, Dept Food Sci, I-43124 Parma, Italy. [Price, Neil P. J.] USDA, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP McCormick, SP (reprint author), USDA, Bacterial Foodbome Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM Susan.McCormick@ars.usda.gov RI Dall'Asta, Chiara/C-3173-2008; OI Dall'Asta, Chiara/0000-0003-0716-8394; Galaverna, Gianni/0000-0001-9042-2378 FU NIH [GM62160] FX D.C. thanks the NIH (GM62160) for support of the synthetic work. NR 39 TC 11 Z9 12 U1 1 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JAN 21 PY 2015 VL 63 IS 2 BP 731 EP 738 DI 10.1021/jf504737f PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AZ6LZ UT WOS:000348332500043 PM 25520274 ER PT J AU Zhao, F Guo, YQ Huang, YB Reddy, KN Zhao, YH Molin, WT AF Zhao, Feng Guo, Yiqing Huang, Yanbo Reddy, Krishna N. Zhao, Yanhua Molin, William T. TI Detection of the onset of glyphosate-induced soybean plant injury through chlorophyll fluorescence signal extraction and measurement SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE chlorophyll fluorescence; soybean injury; herbicide; glyphosate; hyperspectral remote sensing; peak position; blue shift ID CROP INJURY; STRESS; DRIFT; REFLECTANCE; RESPONSES; SCALE; MODEL AB In this study, chlorophyll fluorescence (ChlF) was used to detect the onset of soybean plant injury from treatment of glyphosate, the most widely used herbicide. Thirty-six pots of nonglyphosate-resistant soybean were randomly divided into three groups and treated with different doses of glyphosate solutions. The three treatment groups were control (CTRL) group (with no glyphosate treatment), 0.25X group (treated with 0.217 kg . ae/ha solution of glyphosate), and 0.5X group (treated with 0.433 kg . ae/ha solution of glyphosate). Three kinds of fluorescence measurements, steady-state fluorescence spectra, Kautsky effect parameters, and ChlF-related spectral indices were extracted and generated from the measurements in the glyphosate treatment experiment. The mean values of these fluorescence measurements for each of the CTRL group, the 0.25X group, and the 0.5X group were calculated. Glyphosate-induced leaf injury was then analyzed by examining the separability of these mean values at 6, 24, 48, and 72 hours after the treatment (HAT). Results indicate that the peak position of far-red ChlF shows an obvious blue shift for glyphosate-treated soybean, and peak values of steady-state fluorescence spectra for the three groups can be significantly distinguished from each other at 48 HAT and later. Four Kautsky effect parameters, Fv, Fv/Fm, Area, and PI, are parameters sensitive to glyphosate treatment, showing some differences between the CTRL group and treated groups at 24 HAT, and significant differences among the three groups at and beyond 48 HAT. Moreover, ChlF-related spectral indices, R-683(2)/(R-675 . R-690) and R-690/R-655, are also shown to be useful in detection of the glyphosate injury, though they are less effective than the steady-state fluorescence spectra and the Kautsky effect parameters. Based on the presented results, it can be concluded that glyphosate-induced soybean injury can be detected in a timely manner by the ChlF measurements, and this method has the potential to be further developed into practical use. (C) 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) C1 [Zhao, Feng; Guo, Yiqing] Beihang Univ, Sch Instrumentat Sci & Optoelect Engn, Beijing 100191, Peoples R China. [Huang, Yanbo; Reddy, Krishna N.; Molin, William T.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Zhao, Yanhua] Beijing Inst Space Mech & Elect, Beijing 100094, Peoples R China. RP Huang, YB (reprint author), USDA ARS, Crop Prod Syst Res Unit, Expt Stn Rd, Stoneville, MS 38776 USA. EM yanbo.huang@ars.usda.gov FU Chinese Natural Science Foundation [41371325, 40901156]; State Key Laboratory of Remote Sensing Science [2009kfjj022]; Civil Aerospace Technology Pre-research Project of China [D040201-03]; United States Department of Agriculture Agricultural Research service FX This work is jointly supported by the Chinese Natural Science Foundation under Project Nos. 41371325 and 40901156, the State Key Laboratory of Remote Sensing Science (Grant No. 2009kfjj022), the Civil Aerospace Technology Pre-research Project of China (Grant No. D040201-03), and United States Department of Agriculture Agricultural Research service. Thank Mr. Efren Ford for his works in crop planting, herbicide treatment and laboratory measurements and Mrs. Paige Goodlett for her assistance in laboratory measurements. The authors are thankful to the anonymous reviewers who provided constructive comments to improve this manuscript. NR 31 TC 0 Z9 0 U1 6 U2 24 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD JAN 21 PY 2015 VL 9 AR 097098 DI 10.1117/1.JRS.9.097098 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA CA9CT UT WOS:000349218700001 ER PT J AU Ali, S Magne, M Chen, SY Cote, O Stare, BG Obradovic, N Jamshaid, L Wang, XH Belair, G Moffett, P AF Ali, Shawkat Magne, Maxime Chen, Shiyan Cote, Olivier Stare, Barbara Geric Obradovic, Natasa Jamshaid, Lubna Wang, Xiaohong Belair, Guy Moffett, Peter TI Analysis of Putative Apoplastic Effectors from the Nematode, Globodera rostochiensis, and Identification of an Expansin-Like Protein That Can Induce and Suppress Host Defenses SO PLOS ONE LA English DT Article ID DISEASE RESISTANCE; VIRUS-RESISTANCE; HETERODERA-GLYCINES; TRIGGERED IMMUNITY; PARASITISM FACTOR; PLANT-PATHOGENS; PECTATE LYASE; COAT PROTEIN; POTATO; TOMATO AB The potato cyst nematode, Globodera rostochiensis, is an important pest of potato. Like other pathogens, plant parasitic nematodes are presumed to employ effector proteins, secreted into the apoplast as well as the host cytoplasm, to alter plant cellular functions and successfully infect their hosts. We have generated a library of ORFs encoding putative G. rostochiensis putative apoplastic effectors in vectors for expression in planta. These clones were assessed for morphological and developmental effects on plants as well as their ability to induce or suppress plant defenses. Several CLAVATA3/ESR-like proteins induced developmental phenotypes, whereas predicted cell wall-modifying proteins induced necrosis and chlorosis, consistent with roles in cell fate alteration and tissue invasion, respectively. When directed to the apoplast with a signal peptide, two effectors, an ubiquitin extension protein (GrUBCEP12) and an expansin-like protein (GrEXPB2), suppressed defense responses including NB-LRR signaling induced in the cytoplasm. GrEXPB2 also elicited defense response in species-and sequence-specific manner. Our results are consistent with the scenario whereby potato cyst nematodes secrete effectors that modulate host cell fate and metabolism as well as modifying host cell walls. Furthermore, we show a novel role for an apoplastic expansin-like protein in suppressing intra-cellular defense responses. C1 [Ali, Shawkat; Magne, Maxime; Cote, Olivier; Obradovic, Natasa; Jamshaid, Lubna; Moffett, Peter] Univ Sherbrooke, Dept Biol, Sherbrooke, PQ J1K 2R1, Canada. [Ali, Shawkat; Belair, Guy] Agr & Agri Food Canada, Hort R&D Ctr, St Jean, PQ, Canada. [Chen, Shiyan; Wang, Xiaohong] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Stare, Barbara Geric] Agr Inst Slovenia, Dept Plant Protect, SI-1000 Ljubljana, Slovenia. [Wang, Xiaohong] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Moffett, P (reprint author), Univ Sherbrooke, Dept Biol, 2500 Blvd Univ Sherbrooke, Sherbrooke, PQ J1K 2R1, Canada. EM peter.moffett@usherbrooke.ca OI Moffett, Peter/0000-0003-0678-4531 FU Agriculture and Agri-food Canada Growing Canadian Agri-Innovations Program; National Sciences and Engineering Research Council (NSERC); US Department of Agriculture, Agricultural Research Service; visiting fellowships in government laboratories program (NSERC); Fonds de Recherche Quebecois Nature et Technologie (FRQNT) FX This work was supported by funds from the Agriculture and Agri-food Canada Growing Canadian Agri-Innovations Program to G.B. and P.M., a discovery grant from the National Sciences and Engineering Research Council (NSERC) to P.M., and funds from the US Department of Agriculture, Agricultural Research Service to X.W.S.A. was supported by the visiting fellowships in government laboratories program (NSERC) and by a postdoctoral fellowship from the Fonds de Recherche Quebecois Nature et Technologie (FRQNT), and this work benefited from links established under CropSustaIn FP7 Project. NR 69 TC 8 Z9 8 U1 13 U2 46 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JAN 21 PY 2015 VL 10 IS 1 AR e0115042 DI 10.1371/journal.pone.0115042 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AZ4PR UT WOS:000348205300009 PM 25606855 ER PT J AU Koh, CY Schaff, UY Piccini, ME Stanker, LH Cheng, LW Ravichandran, E Singh, BR Sommer, GJ Singh, AK AF Koh, Chung-Yan Schaff, Ulrich Y. Piccini, Matthew E. Stanker, Larry H. Cheng, Luisa W. Ravichandran, Easwaran Singh, Bal-Ram Sommer, Greg J. Singh, Anup K. TI Centrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin SO ANALYTICAL CHEMISTRY LA English DT Article ID SINGLE-DOMAIN ANTIBODIES; CLOSTRIDIUM-BOTULINUM; QUANTITATIVE DETECTION; BIOLOGICAL SAMPLES; ORAL TOXICITIES; A NEUROTOXIN; ASSAY; FOOD; PCR; CONFIRMATION AB We present an innovative centrifugal microfluidic immunoassay platform (SpinDx) to address the urgent biodefense and public health need for ultrasensitive point-of-care/incident detection of botulinum toxin. The simple, sample-to-answer centrifugal microfluidic immunoassay approach is based on binding of toxins to antibody-laden capture particles followed by sedimentation of the particles through a density-media in a microfluidic disk and quantification by laser-induced fluorescence. A blind, head-to-head comparison study of SpinDx versus the gold-standard mouse bioassay demonstrates 100-fold improvement in sensitivity (limit of detection = 0.09 pg/mL), while achieving total sample-to-answer time of <30 min with 2-mu L required volume of the unprocessed sample. We further demonstrate quantification of botulinum toxin in both exogeneous (human blood and serum spiked with toxins) and endogeneous (serum from mice intoxicated via oral, intranasal, and intravenous routes) samples. SpinDx can analyze, without any sample preparation, multiple sample types including whole blood, serum, and food. It is readily expandable to additional analytes as the assay reagents (i.e., the capture beads and detection antibodies) are disconnected from the disk architecture and the reader, facilitating rapid development of new assays. SpinDx can also serve as a general-purpose immunoassay platform applicable to diagnosis of other conditions and diseases. C1 [Koh, Chung-Yan; Schaff, Ulrich Y.; Piccini, Matthew E.; Sommer, Greg J.; Singh, Anup K.] Sandia Natl Labs, Livermore, CA 94551 USA. [Stanker, Larry H.; Cheng, Luisa W.] USDA ARS, Western Reg Res Ctr, Foodborne Contaminants Res Unit, Albany, CA 94710 USA. [Ravichandran, Easwaran; Singh, Bal-Ram] Univ Massachusetts Dartmouth, N Dartmouth, MA 02747 USA. RP Koh, CY (reprint author), Sandia Natl Labs, 7011 East Ave, Livermore, CA 94551 USA. EM ckoh@sandia.gov; aksingh@sandia.gov RI Ravichandran, Easwaran/N-7239-2016 OI Ravichandran, Easwaran/0000-0003-1613-0339 FU National Institute of Allergies and Infectious Disease (NIAID) [U01A1075441, R01AI098853]; United States Department of Agriculture, Agricultural Research Service, National Program project [NP108]; CRIS [5325-42000-043-00D]; National Institute of Allergy and Infectious Diseases Service [U54 AI065359]; U.S. Department of Energy's National Nuclear Security Administration [DE-AC04-94AL85000 - SAND2014-20313J] FX This work was funded by the National Institute of Allergies and Infectious Disease (NIAID) Grants U01A1075441 and R01AI098853. L.W.C. and L.H.S. were funded by the United States Department of Agriculture, Agricultural Research Service, National Program project NP108, CRIS 5325-42000-043-00D and the National Institute of Allergy and Infectious Diseases Service Grant U54 AI065359. Sandia National Laboratories is a multiprogram laboratory managed and operated by Sandia Corporation, a wholly owned subsidiary of Lockheed Martin Corporation, for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-AC04-94AL85000 - SAND2014-20313J. NR 49 TC 10 Z9 10 U1 13 U2 49 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD JAN 20 PY 2015 VL 87 IS 2 BP 922 EP 928 DI 10.1021/ac504054u PG 7 WC Chemistry, Analytical SC Chemistry GA AZ6LX UT WOS:000348332300015 PM 25521812 ER PT J AU Sakihara, TS Dudley, BD MacKenzie, RA Beets, JP AF Sakihara, T. S. Dudley, B. D. MacKenzie, R. A. Beets, J. P. TI Endemic grazers control benthic microalgal growth in a eutrophic tropical brackish ecosystem SO MARINE ECOLOGY PROGRESS SERIES LA English DT Article DE Anchialine pool; Top-down control; Bottom-up control; Epilithon; Grazing; Nutrient enrichment; Halocaridina rubra ID SHRIMP HALOCARIDINA-RUBRA; SUBMARINE GROUNDWATER DISCHARGE; NUTRIENT ENRICHMENT; INTRODUCED FISH; CORAL-REEFS; HAWAII; PERIPHYTON; CONSEQUENCES; GRADIENTS; COMMUNITY AB Anthropogenic changes to nutrient supply, numbers and behavior of grazers and interactions of these factors are known to change epilithon composition and biomass. In brackish waters, these changes occur across wide-ranging abiotic conditions (e.g. nutrient concentrations and salinity), which may alter their relative impacts on microphytobenthic communities. Such mediating processes are poorly understood, particularly in tropical brackish ecosystems. We examined the separate and interactive effects of nutrient additions and grazer exclusion on epilithon composition and biomass in eutrophic Hawaiian anchialine pools between contrasting levels of salinity and concomitant nutrient-rich groundwater influence (2.3 to 22.0 ppt; nitrite + nitrate, 6.3 to 102.6 mu M; soluble reactive phosphorus, < 0.5 to 5.18 mu M). Across these conditions, we found no significant effects of nitrogen (N) and phosphorus (P) enrichment treatments on chlorophyll a, biomass (ash-free dry mass) or autotrophic index (i.e. autotrophy) of epilithon communities, and nutrient addition did not alter the effects of grazing pressure. However, autotrophy and epilithon biomass were lower in low salinity, high groundwater influenced pools, and both were strongly reduced by grazing in all pools. Furthermore, effects from grazing pressure on relative autotrophy were more pronounced in low salinity, high groundwater influenced pools. Our results suggest that (1) grazing is the primary driver of epilithon composition and biomass across these nutrient-enriched systems, (2) microphytobenthic communities in these systems are not N or P limited irrespective of co-varying salinity and background nutrients and (3) selective feeding by endemic grazers mitigates potential bottom-up forces from salinity or increased nutrient-enriched groundwater influence on autotrophy in epilithon communities. C1 [Sakihara, T. S.; Beets, J. P.] Univ Hawaii, Trop Conservat Biol & Environm Sci Program, Hilo, HI 96720 USA. [Dudley, B. D.] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. [MacKenzie, R. A.] USDA, Forest Serv, Pacific Southwest Res Stn, Inst Pacific Isl Forestry, Hilo, HI 96720 USA. RP Sakihara, TS (reprint author), Univ Hawaii, Trop Conservat Biol & Environm Sci Program, 200 West Kawili St, Hilo, HI 96720 USA. EM tsakihar@hawaii.edu FU National Science Foundation (Hawai'i EPSCoR NSF) [EPS-0903833]; Division of Forestry and Wildlife, Natural Area Re serves System, State of Hawaii FX We thank the following for their invaluable support, expertise and assistance in the field and laboratory: Robert Nishimoto, Troy Shimoda, Timothy Shindo and Lance Nishiura of the State of Hawai'i Division of Aquatic Resources (DAR); Lucas Mead and Tara Holitzki of the University of Hawai'i at Hilo Analytical Lab; Therese Frauendorf, Alison Sherwood and Ayron Strauch of the University of Hawai'i at Manoa; Amy Korwin, Ambyr Mokiao-Lee, Kehau Nelson-Kaula and Rebecca Ostertag of the University of Hawai'i at Hilo; Rebecca Most of The Nature Conservancy; James Baldwin, Flint Hughes and Michael Riney of the USDA Forest Service; Nelson Hairston Jr. of Cornell University; and Lono Humphreys. Permissions for access and sampling within the Manuka NAR were granted by the Division of Forestry and Wildlife, Natural Area Re serves System, State of Hawaii. Funding was provided by the National Science Foundation (Hawai'i EPSCoR NSF Grant No. EPS-0903833). Logistical support was provided by DAR and the USDA Forest Service. NR 72 TC 4 Z9 4 U1 0 U2 15 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0171-8630 EI 1616-1599 J9 MAR ECOL PROG SER JI Mar. Ecol.-Prog. Ser. PD JAN 20 PY 2015 VL 519 BP 29 EP 45 DI 10.3354/meps11099 PG 17 WC Ecology; Marine & Freshwater Biology; Oceanography SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography GA CA7JO UT WOS:000349093800003 ER PT J AU Hines, SA Ramsay, JD Kappmeyer, LS Lau, AOT Ojo, KK Van Voorhis, WC Knowles, DP Mealey, RH AF Hines, Siddra A. Ramsay, Joshua D. Kappmeyer, Lowell S. Lau, Audrey O. T. Ojo, Kayode K. Van Voorhis, Wesley C. Knowles, Donald P. Mealey, Robert H. TI Theileria equi isolates vary in susceptibility to imidocarb dipropionate but demonstrate uniform in vitro susceptibility to a bumped kinase inhibitor SO PARASITES & VECTORS LA English DT Article DE Theileria equi; Equine piroplasmosis; Imidocarb dipropionate; Apicomplexan; Bumped kinase inhibitor; Drug susceptibility ID PIROPLASMOSIS BABESIA-EQUI; TOXOPLASMA-GONDII; INFECTED HORSES; FLOW-CYTOMETRY; PARASITES; GROWTH; EFFICACY; RESISTANCE; GIBSONI; PYRIMETHAMINE AB Background: The apicomplexan hemoparasite Theileria equi is a causative agent of equine piroplasmosis, eradicated from the United States in 1988. However, recent outbreaks have sparked renewed interest in treatment options for infected horses. Imidocarb dipropionate is the current drug of choice, however variation in clinical response to therapy has been observed. Methods: We quantified the in vitro susceptibility of two T. equi isolates and a lab generated variant to both imidocarb dipropionate and a bumped kinase inhibitor compound 1294. We also evaluated the capacity of in vitro imidocarb dipropionate exposure to decrease susceptibility to that drug. The efficacy of imidocarb dipropionate for clearing infection in four T. equi infected ponies was also assessed. Results: We observed an almost four-fold difference in imidocarb dipropionate susceptibility between two distinct isolates of T. equi. Four ponies infected with the less susceptible USDA Florida strain failed to clear the parasite despite two rounds of treatment. Importantly, a further 15-fold decrease in susceptibility was produced in this strain by continuous in vitro imidocarb dipropionate exposure. Despite a demonstrated difference in imidocarb dipropionate susceptibility, there was no difference in the susceptibility of two T. equi isolates to bumped kinase inhibitor 1294. Conclusions: The observed variation in imidocarb dipropionate susceptibility, further reduction in susceptibility caused by drug exposure in vitro, and failure to clear T. equi infection in vivo, raises concern for the emergence of drug resistance in clinical cases undergoing treatment. Bumped kinase inhibitors may be effective as alternative drugs for the treatment of resistant T. equi parasites. C1 [Hines, Siddra A.; Ramsay, Joshua D.; Kappmeyer, Lowell S.; Lau, Audrey O. T.; Knowles, Donald P.; Mealey, Robert H.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Kappmeyer, Lowell S.; Knowles, Donald P.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [Ojo, Kayode K.; Van Voorhis, Wesley C.] Univ Washington, Sch Med, Div Allergy & Infect Dis, Seattle, WA 98109 USA. [Ojo, Kayode K.; Van Voorhis, Wesley C.] Univ Washington, Sch Med, Ctr Emerging & Reemerging Infect Dis, Seattle, WA 98109 USA. RP Hines, SA (reprint author), Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM siddra@vetmed.wsu.edu; rhm@vetmed.wsu.edu FU Zoetis-Morris Animal Foundation Fellowship [D12EQ-901]; USDA-NIFA-AFRI Fellowship [2012-67011-19946]; Grayson Jockey Club Research Foundation; USDA-NIFA-AFRI [2013-01149]; NIH [1 R01 AI089441, AI111341-01]; USDA [2014-06183] FX This work was supported in part by Zoetis-Morris Animal Foundation Fellowship Grant No. D12EQ-901 (SAH), USDA-NIFA-AFRI Fellowship Grant No. 2012-67011-19946 (JDR), the Grayson Jockey Club Research Foundation (RHM), and USDA-NIFA-AFRI Grant No. 2013-01149 (RHM). Work in the Van Voorhis lab was supported by NIH grants 1 R01 AI089441, AI111341-01 and USDA grant # 2014-06183. NR 54 TC 3 Z9 3 U1 2 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD JAN 20 PY 2015 VL 8 AR 33 DI 10.1186/s13071-014-0611-6 PG 11 WC Parasitology SC Parasitology GA CB1VP UT WOS:000349416400001 PM 25600252 ER PT J AU Sarikaya-Bayram, O Palmer, JM Keller, N Braus, GH Bayram, O AF Sarikaya-Bayram, Oezlem Palmer, Jonathan M. Keller, Nancy Braus, Gerhard H. Bayram, Oezguer TI One Juliet and four Romeos: VeA and its methyltransferases SO Frontiers in Microbiology LA English DT Review DE secondary metabolism; methyltransferases; velvet family; LaeA; VelB-VeA-LaeA; VosA; LlmF; VapA-VipC-VapB ID ASPERGILLUS-NIDULANS; SECONDARY METABOLISM; FUNGAL DEVELOPMENT; SEXUAL DEVELOPMENT; FUNCTIONAL ROLES; GENE CLUSTERS; LAEA; COMPLEX; VIRULENCE; EXPRESSION AB Fungal secondary metabolism has become an important research topic with great biomedical and biotechnological value. In the postgenomic era, understanding the diversity and the molecular control of secondary metabolites (SMs) are two challenging tasks addressed by the research community. Discovery of the LaeA methyltransferase 10 years ago opened up a new horizon on the control of SM research when it was found that expression of many SM gene clusters is controlled by LaeA. While the molecular function of LaeA remains an enigma, discovery of the velvet family proteins as interaction partners further extended the role of the LaeA beyond secondary metabolism. The heterotrimeric VeIB-VeA-LaeA complex plays important roles in development, sporulation, secondary metabolism, and pathogenicity. Recently, three other methyltransferases have been found to associate with the velvet complex, the LaeA-like methyltransferase F and the methyltransferase heterodimers VipC-VapB. Interaction of VeA with at least four methyltransferase proteins indicates a molecular hub function for VeA that questions: Is there a VeA supercornplex or is VeA part of a highly dynamic cellular control network with many different partners? C1 [Sarikaya-Bayram, Oezlem; Bayram, Oezguer] Natl Univ Ireland, Maynooth Univ, Dept Biol, Maynooth, Kildare, Ireland. [Palmer, Jonathan M.] US Forest Serv, Northern Res Stat, Ctr Forest Mycol Res, Madison, WI USA. [Keller, Nancy] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA. [Braus, Gerhard H.] Univ Gottingen, Dept Microbiol & Mol Genet, D-37073 Gottingen, Germany. RP Bayram, O (reprint author), Natl Univ Ireland, Maynooth Univ, Dept Biol, Maynooth, Kildare, Ireland. EM ozgur.bayram@nuim.ie RI Braus, Gerhard/G-3999-2012; OI Braus, Gerhard/0000-0002-3117-5626; Bayram, Ozgur/0000-0002-0283-5322; Palmer, Jonathan/0000-0003-0929-3658 FU Science Foundation Ireland (SFI) [13/CDA/2142]; National Institutes of Health Grant [NIH R01 A1065728-01]; Deutsche Forschungsgemeinschaft (DFG); Irish Research Council (IRC) [GOIPD/2014/178] FX We would like to thank Betim Karaboda (Maynooth University, Ireland) for critical reading of the manuscript This publication has been supported in part by a research grant from Science Foundation Ireland (SFI) under Grant Number 13/CDA/2142 to Ozgur Bayram, in part by National Institutes of Health Grant NIH R01 A1065728-01 to Nancy Keller and in part by the Deutsche Forschungsgemeinschaft (DFG) to Gerhard FL Brans. (Ozlem Sarikaya-Bayram has been supported by the Irish Research Council (IRC) Postdoctoral Fellowship (GOIPD/2014/178). NR 45 TC 16 Z9 16 U1 5 U2 22 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD JAN 20 PY 2015 VL 6 AR 1 DI 10.3389/fmicb.2015.00001 PG 7 WC Microbiology SC Microbiology GA CA4FF UT WOS:000348859100001 PM 25653648 ER PT J AU Marble, SC Prior, SA Runion, GB Torbert, HA AF Marble, S. Christopher Prior, Stephen A. Runion, G. Brett Torbert, H. Allen TI Control of yellow and purple nutsedge in elevated CO2 environments with glyphosate and halosulfuron SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE climate change; invasive species; herbicide; nutsedge; resistance management ID ATMOSPHERIC CARBON-DIOXIDE; THISTLE CIRSIUM-ARVENSE; CYPERUS-ESCULENTUS; INCREASED TOLERANCE; INVASIVE WEED; GROWTH; RESPONSES; ROTUNDUS; ENRICHMENT; PLANTS AB Atmospheric concentrations of carbon dioxide (CO2) have significantly increased over the past century and are expected to continue rising in the future. While elevated levels of CO2 will likely result in higher crop yields, weed growth is also highly likely to increase, which could increase the incidence of herbicide resistant biotypes. An experiment was conducted in 2012 to determine the effects of an elevated CO2 environment on glyphosate and halosulfuron efficacy for postemergence control of purple and yellow nutsedge (Cyperus rotundus L. and C. esculentus L.). Both species of nutsedge where grown in 3.0-L containers under either ambient or elevated (ambient + 200 mu mol mol(-1)) CO2 in open-top field chambers and treated with either 0.5x, 1.0x, or 1.5x of the manufacturer's labeled rate of halosulfuron, glyphosate, or a tank mix of the two herbicides. The growth of both nutsedge species responded positively to elevated CO2, purple nutsedge had increased shoot and root dry weights and yellow nutsedge had increased shoot, root, and tuber dry weights and counts. Few treatment differences were observed among the herbicides at any of the rates tested. At 3 weeks following herbicide application, both purple and yellow nutsedge were adequately controlled by both herbicides and combinations at all rates tested, regardless of CO2 concentration. Based on this study, it is likely that predicted future CO2 levels will have little impact on the efficacy of single applications of halosulfuron or glyphosate for control of purple and yellow nutsedge at the growth stages described here, although scenarios demanding more persistent control efforts remain a question. C1 [Marble, S. Christopher] Univ Florida, Inst Food & Agr Sci, Mid Florida Res & Educ Ctr, Dept Environm Hort, Apopka, FL 32703 USA. [Prior, Stephen A.; Runion, G. Brett; Torbert, H. Allen] USDA, Natl Soil Dynam Lab, Agr Res Serv, Auburn, AL USA. RP Marble, SC (reprint author), Univ Florida, Inst Food & Agr Sci, Mid Florida Res & Educ Ctr, Dept Environm Hort, 2725 S Binion Rd, Apopka, FL 32703 USA. EM marblesc@ufl.edu NR 42 TC 4 Z9 4 U1 2 U2 25 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JAN 20 PY 2015 VL 6 AR 1 DI 10.3389/fpls.2015.00001 PG 6 WC Plant Sciences SC Plant Sciences GA AZ1ZK UT WOS:000348033700001 PM 25653664 ER PT J AU Uramoto, K Zucchi, RA Norrbom, AL AF Uramoto, Keiko Zucchi, Roberto A. Norrbom, Allen L. TI Redescription of three species of Anastrepha (Diptera, Tephritidae) rediscovered in Brazil, with the establishment of a new synonym SO ZOOTAXA LA English DT Article DE Diptera; Tephritidae ID ATLANTIC RAIN-FOREST; ESPIRITO-SANTO; REMNANT AREA; STATE AB The descriptions of Anastrepha matertela Zucchi and A. tenella Zucchi were based exclusively on the holotypes (female). Based on additional specimens collected in Brazil since their original descriptions, both species are redescribed and illustrated. A lectotype is designated for Anastrepha bivittata (Macquart, 1843), which also is redescribed and considered to be the senior synonym of A. fumipennis Lima, 1934. C1 [Uramoto, Keiko; Zucchi, Roberto A.] Univ Sao Paulo, Dept Entomol & Acarol, ESALQ, BR-13418900 Piracicaba, SP, Brazil. [Norrbom, Allen L.] USDA ARS, Systemat Entomol Lab, Washington, DC 20013 USA. RP Uramoto, K (reprint author), Univ Sao Paulo, Dept Entomol & Acarol, ESALQ, Caixa Postal 9, BR-13418900 Piracicaba, SP, Brazil. EM uramoto@usp.br; razucchi@usp.br; allen.norrbom@ars.usda.gov RI Zucchi, Roberto/D-7738-2012 OI Zucchi, Roberto/0000-0001-9861-7460 FU CNPq [303726/2009-1] FX We are grateful to Pascal De Bleecker, Museum d'Histoire Naturelle, Lille, for access to the Macquart Collection and help with photography of the A. bivittata type. Thanks are also due to David S. Martins, Maria Aparecida L. Bittencourt and Maria C. A. Nunes, collectors of A. bivittata, A. matertela and A. tenella, respectively, for sending the specimens for our study. We also thank Francisco Andre Ossamu Tanaka who kindly took pictures in the stereomicroscope and for the permission to use the microscopes at the Nucleo de Apoio a Pesquisa em Microscopia Eletronica Aplicada a Pesquisa Agropecuaria, ESALQ/USP. RAZ is a fellow of the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). This study was supported in part by CNPq (grant 303726/2009-1). USDA is an equal opportunity provider and employer. NR 21 TC 0 Z9 0 U1 0 U2 2 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD JAN 20 PY 2015 VL 3911 IS 3 BP 411 EP 423 PG 13 WC Zoology SC Zoology GA AZ1EH UT WOS:000347982500007 PM 25661621 ER PT J AU Antoniou, J Liu, F Majeed, H Qi, J Yokoyama, W Zhong, F AF Antoniou, John Liu, Fei Majeed, Hamid Qi, Jing Yokoyama, Wallace Zhong, Fang TI Physicochemical and morphological properties of size-controlled chitosan-tripolyphosphate nanoparticles SO COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS LA English DT Article DE Chitosan nanoparticles; Ionic crosslinking; Ionic strength; Particle size; Morphology; Ultra-sonication ID PROTEIN DELIVERY; DRUG-DELIVERY; RELEASE; FILMS; CARRIERS; COMPLEX; CHITIN; CHARGE AB Chitosan-tripolyphosphate nanoparticles have been extensively studied during the last decade because of their numerous applications. In this study, we describe conditions to optimize chitosan nanoparticles as potential nano-fillers in edible films. The ionic cross-linking between the cationic amino groups on the chitosan (CS) chain and the anionic phosphate groups of sodium tripolyphosphate (TPP) was verified via FTIR. Particle size, polydispersity index (PDI) and surface zeta-potential were controlled by chitosan's Mw and concentration, CS: TPP mass ratio, and external conditions such as pH and salinity of the initial chitosan solution. Post-processing methods such as centrifugation and ultra-sonication were used to further control particle size. We show that particle size can be controlled by selecting appropriate conditions. Particles with sizes below 120 nm were produced at different CS: TPP mass ratios depending on the CS concentration. Dilute NaCl was the optimal solution ionic composition that decreased the size by 25% and also resulted in a narrow particle size distribution. We show using UV-vis spectrophotometry that particles of different size, separated by centrifugation had different phosphorus content. Ultra-sonication can be used to reduce the size by 50% but long time caused fragmentation of the nanoparticles. Transmission electron microscopy (TEM) revealed the differences in the morphology of chitosan nanoparticles under various fabrication conditions. (C) 2014 Elsevier B.V. All rights reserved. C1 [Antoniou, John; Liu, Fei; Majeed, Hamid; Qi, Jing; Zhong, Fang] Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. [Yokoyama, Wallace] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn FU National 863 Program [2011BAD23B02, 2013AA102207]; NSFC [31171686, 30901000]; 111 project [B07029, PCSIRT0627] FX This work was financially supported by National 863 Program 2011BAD23B02, 2013AA102207, NSFC 31171686, 30901000, 111 project-B07029 and PCSIRT0627 NR 40 TC 17 Z9 17 U1 7 U2 60 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0927-7757 EI 1873-4359 J9 COLLOID SURFACE A JI Colloid Surf. A-Physicochem. Eng. Asp. PD JAN 20 PY 2015 VL 465 BP 137 EP 146 DI 10.1016/j.colsurfa.2014.10.040 PG 10 WC Chemistry, Physical SC Chemistry GA AU4CM UT WOS:000345556500018 ER PT J AU Dowd, PF Sattler, SE AF Dowd, Patrick F. Sattler, Scott E. TI Helicoverpa zea (Lepidoptera: Noctuidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae) Responses to Sorghum bicolor (Poales: Poaceae) Tissues From Lowered Lignin Lines SO JOURNAL OF INSECT SCIENCE LA English DT Article DE resistance; agricultural entomology; plant resistance ID CINNAMYL-ALCOHOL-DEHYDROGENASE; BROWN-MIDRIB GENES; REDUCED LIGNIN; FALL ARMYWORM; MAIZE; L.; BIOSYNTHESIS; EXPRESSION; RESISTANCE; PHENOTYPE AB The presence of lignin within biomass impedes the production of liquid fuels. Plants with altered lignin content and composition are more amenable to lignocellulosic conversion to ethanol and other biofuels but may be more susceptible to insect damage where lignin is an important resistance factor. However, reduced lignin lines of switchgrasses still retained insect resistance in prior studies. Therefore, we hypothesized that sorghum lines with lowered lignin content will also retain insect resistance. Sorghum excised leaves and stalk pith Sorghum bicolor (L.) Moench (Poales: Poaceae) from near isogenic brown midrib (bmr) 6 and 12 mutants lines, which have lowered lignin content and increased lignocellulosic ethanol conversion efficiency, were examined for insect resistance relative to wild-type (normal BTx623). Greenhouse and growth chamber grown plant tissues were fed to first-instar larvae of corn earworms, Helicoverpa zea (Boddie) and fall armyworms Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), two sorghum major pests. Younger bmr leaves had significantly greater feeding damage in some assays than wild-type leaves, but older bmr6 leaves generally had significantly less damage than wild-type leaves. Caterpillars feeding on the bmr6 leaves often weighed significantly less than those feeding on wild-type leaves, especially in the S. frugiperda assays. Larvae fed the pith from bmr stalks had significantly higher mortality compared with those larvae fed on wild-type pith, which suggested that bmr pith was more toxic. Thus, reducing lignin content or changing subunit composition of bioenergy grasses does not necessarily increase their susceptibility to insects and may result in increased resistance, which would contribute to sustainable production. C1 [Dowd, Patrick F.] USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. [Sattler, Scott E.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA ARS, Lincoln, NE 68583 USA. RP Dowd, PF (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM patrick.dowd@ars.usda.gov FU Agricultural Food Research Institute from the National Institute of Food and Agriculture [2011-67009-30026]; Agricultural Research Service FX We thank B.T. Ade, Z. Demkovitch, D. Lee, and A. Maybanks (USDA-ARS, National Center for Agricultural Utilization Research) for technical assistance, D. Palmquist (USDA-ARS, Midwest Area Biometrician) for suggestions on statistical analyses, and J.F. Pedersen (USDA-ARS, University of Nebraska-Lincoln, retired), A.P. Rooney (USDA-ARS, National Center for Agricultural Utilization Research), and F.E. Vega (USDA-ARS, Beltsville Agricultural Research Center) for comments on prior versions of the manuscript. This work was supported by Agricultural Food Research Institute award number 2011-67009-30026 from the National Institute of Food and Agriculture and funding the base Agricultural Research Service projects. NR 33 TC 3 Z9 3 U1 2 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD JAN 19 PY 2015 VL 15 DI 10.1093/jisesa/ieu162 PG 5 WC Entomology SC Entomology GA CD1OS UT WOS:000350844800001 ER PT J AU Alvarado-Esquivel, C Alvarado-Esquivel, D Dubey, JP AF Alvarado-Esquivel, Cosme Alvarado-Esquivel, Domingo Dubey, Jitender P. TI Prevalence of Toxoplasma gondii antibodies in domestic donkeys (Equus asinus) in Durango, Mexico slaughtered for human consumption SO BMC Veterinary Research LA English DT Article DE Toxoplasma gondii; Seroprevalence; Donkeys; Cross-sectional study; Mexico ID SEROPREVALENCE; HORSES; PROVINCE; EQUIDS; CHINA AB Background: Nothing is known about Toxoplasma gondii prevalence in donkeys in Mexico. Meat from donkey is consumed by humans in Mexico and also exported to other countries. We sought to determine the presence of antibodies against T. gondii in 239 domestic donkeys (Equus asinus) for slaughter in Durango, Mexico using the modified agglutination test (MAT). Donkeys were sampled in four premises (trade centers) where donkeys were gather for shipment to abattoirs in other Mexican states. Results: Antibodies to T. gondii were found in 26 (10.9%) of 239 donkeys, with MAT titers of 1:25 in 7, 1:50 in 11, 1:100 in 6, and 1:200 in 2. Seropositive donkeys were found in three (75%) of the four gathering premises studied. Seroprevalence in donkeys varied from 0% to 23.1% among gathering premises. The seroprevalence of T. gondii infection was comparable among donkeys regardless their age, sex or health status. Seropositivity to T. gondii was found in donkeys between 1 to 12 years old. Multivariate analysis showed that seropositivity to T. gondii was associated with the gathering premises (OR = 1.58; 95% CI: 1.11-2.24; P = 0.009). Conclusions: This is the first report of T. gondii infection in donkeys in Mexico. Results indicate that consumption of undercooked or raw meat from T. gondii-infected donkeys is potentially a source of T. gondii infection for humans. C1 [Alvarado-Esquivel, Cosme; Alvarado-Esquivel, Domingo] Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Durango 34000, Mexico. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Alvarado-Esquivel, C (reprint author), Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Ave Univ S-N, Durango 34000, Mexico. EM alvaradocosme@yahoo.com FU Juarez University of Durango State FX This study was supported by Juarez University of Durango State. NR 19 TC 1 Z9 1 U1 0 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD JAN 17 PY 2015 VL 11 AR 6 DI 10.1186/s12917-015-0325-9 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA CA3RF UT WOS:000348823200002 PM 25595816 ER PT J AU Mallia, DV Lin, JC Urbanski, S Ehleringer, J Nehrkorn, T AF Mallia, D. V. Lin, J. C. Urbanski, S. Ehleringer, J. Nehrkorn, T. TI Impacts of upwind wildfire emissions on CO, CO2, and PM2.5 concentrations in Salt Lake City, Utah SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE biomass burning emissions; air quality; land-atmosphere exchange; Lagrangian particle dispersion models; Weather Research and Forecasting Model; trajectory modeling ID WESTERN UNITED-STATES; AIR-QUALITY; ATMOSPHERIC OBSERVATIONS; SOURCE APPORTIONMENT; TRANSPORT MODELS; FOREST INVENTORY; CARBON-MONOXIDE; US; FIRES; SCALE AB Biomass burning is known to contribute large quantities of CO2, CO, and PM2.5 to the atmosphere. Biomass burning not only affects the area in the vicinity of fire but may also impact the air quality far downwind from the fire. The 2007 and 2012 western U.S. wildfire seasons were characterized by significant wildfire activity across much of the Intermountain West and California. In this study, we determined the locations of wildfire-derived emissions and their aggregate impacts on Salt Lake City, a major urban center downwind of the fires. To determine the influences of biomass burning emissions, we initiated an ensemble of stochastic back trajectories at the Salt Lake City receptor within the Stochastic Time-Inverted Lagrangian Transport (STILT) model, driven by wind fields from the Weather Research and Forecasting (WRF) model. The trajectories were combined with a new, high-resolution biomass burning emissions inventorythe Wildfire Emissions Inventory. Initial results showed that the WRF-STILT model was able to replicate many periods of enhanced wildfire activity observed in the measurements. Most of the contributions for the 2007 and 2012 wildfire seasons originated from fires located in Utah and central Idaho. The model results suggested that during intense episodes of upwind wildfires in 2007 and 2012, fires contributed as much as 250ppb of CO during a 3 h period and 15 mu g/m(3) of PM2.5 averaged over 24 h at Salt Lake City. Wildfires had a much smaller impact on CO2 concentrations in Salt Lake City, with contributions rarely exceeding 2ppm enhancements. Key Points C1 [Mallia, D. V.; Lin, J. C.] Univ Utah, Dept Atmospher Sci, Salt Lake City, UT 84112 USA. [Urbanski, S.] US Forest Serv, Missoula Fire Sci Lab, Rocky Mt Res Stn, Missoula, MT USA. [Ehleringer, J.] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA. [Nehrkorn, T.] Atmospher & Environm Res Inc, Lexington, MA USA. RP Mallia, DV (reprint author), Univ Utah, Dept Atmospher Sci, Salt Lake City, UT 84112 USA. EM Derek.Mallia@utah.edu OI Nehrkorn, Thomas/0000-0003-0637-3468 FU NOAA [NA130AR4310087]; Utah Division of Air Quality; Office of Science (BER); U.S. Department of Energy [DEFG0206ER64309, DESC0005266] FX This study was made possible by NOAA grant NA130AR4310087 and funding from the Utah Division of Air Quality. We would like to thank the Utah Division of Air Quality for carrying out observations at the Hawthorne site (downloaded from http://www.epa.gov/ttn/airs/airsaqs/) and for providing emission data used in this study. D. Mallia gratefully acknowledges the support of the University of Utah's Global Change and Sustainability Center Graduate Fellowship during his first year of graduate studies. The Salt Lake Valley CO2 observation network was supported by the Office of Science (BER), U.S. Department of Energy, grants DEFG0206ER64309 and DESC0005266 (http://co2.utah.edu). The authors also thank Joshua Benmergui for providing scripts that calculate depositional loss and valuable input from Logan Mitchell, Court Strong, Erik Crosman, John Horel, Adam Kochanski, Deyong Wen, Munkhbayar Baasandorgj, and the University of Utah WRF Users Group. The support and resources from the Center for High Performance Computing at the University of Utah are gratefully acknowledged. The data used to produce the results in this study are available from the corresponding author upon request. Lastly, the authors would like to thank the three anonymous reviewers for their comments and suggestions. NR 91 TC 1 Z9 1 U1 9 U2 45 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD JAN 16 PY 2015 VL 120 IS 1 BP 147 EP 166 DI 10.1002/2014JD022472 PG 20 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA CA7NR UT WOS:000349104700010 ER PT J AU Subramanyam, S Sardesai, N Minocha, SC Zheng, C Shukle, RH Williams, CE AF Subramanyam, Subhashree Sardesai, Nagesh Minocha, Subhash C. Zheng, Cheng Shukle, Richard H. Williams, Christie E. TI Hessian fly larval feeding triggers enhanced polyamine levels in susceptible but not resistant wheat SO BMC PLANT BIOLOGY LA English DT Article DE Polyamines; Wheat; Hessian fly; Compatible; Incompatible; RT-qPCR; Odc; Samdc; Spds ID HYPERSENSITIVE RESPONSE; DIPTERA-CECIDOMYIIDAE; GENE-EXPRESSION; JASMONIC ACID; PLANT DEFENSE; BIOSYNTHESIS; METABOLISM; DISEASE; LEAVES; PUTRESCINE AB Background: Hessian fly (Mayetiola destructor), a member of the gall midge family, is one of the most destructive pests of wheat (Triticum aestivum) worldwide. Probing of wheat plants by the larvae results in either an incompatible (avirulent larvae, resistant plant) or a compatible (virulent larvae, susceptible plant) interaction. Virulent larvae induce the formation of a nutritive tissue, resembling the inside surface of a gall, in susceptible wheat. These nutritive cells are a rich source of proteins and sugars that sustain the developing virulent Hessian fly larvae. In addition, on susceptible wheat, larvae trigger a significant increase in levels of amino acids including proline and glutamic acid, which are precursors for the biosynthesis of ornithine and arginine that in turn enter the pathway for polyamine biosynthesis. Results: Following Hessian fly larval attack, transcript abundance in susceptible wheat increased for several genes involved in polyamine biosynthesis, leading to higher levels of the free polyamines, putrescine, spermidine and spermine. A concurrent increase in polyamine levels occurred in the virulent larvae despite a decrease in abundance of Mdes-odc (ornithine decarboxylase) transcript encoding a key enzyme in insect putrescine biosynthesis. In contrast, resistant wheat and avirulent Hessian fly larvae did not exhibit significant changes in transcript abundance of genes involved in polyamine biosynthesis or in free polyamine levels. Conclusions: The major findings from this study are: (i) although polyamines contribute to defense in some plant-pathogen interactions, their production is induced in susceptible wheat during interactions with Hessian fly larvae without contributing to defense, and (ii) due to low abundance of transcripts encoding the rate-limiting ornithine decarboxylase enzyme in the larval polyamine pathway the source of polyamines found in virulent larvae is plausibly wheat-derived. The activation of the host polyamine biosynthesis pathway during compatible wheat-Hessian fly interactions is consistent with a model wherein the virulent larvae usurp the polyamine biosynthesis machinery of the susceptible plant to acquire nutrients required for their own growth and development. C1 [Subramanyam, Subhashree; Sardesai, Nagesh; Williams, Christie E.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Zheng, Cheng] Purdue Univ, Dept Stat, W Lafayette, IN 47907 USA. [Shukle, Richard H.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Shukle, Richard H.; Williams, Christie E.] USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Williams, CE (reprint author), Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. EM christie.williams@ars.usda.gov FU USDA-CRIS [3602-22000-016-D]; New Hampshire Agricultural Experiment Station (NHAES) FX This work was a joint contribution by the USDA Agricultural Research Service Crop Production and Pest Control Research Unit and Purdue University. Research was supported by USDA-CRIS number 3602-22000-016-D. Partial support for this work was also provided by the New Hampshire Agricultural Experiment Station (NHAES); this is NHAES scientific contribution number 2442. The authors wish to thank Jill Nemacheck (USDA-ARS) for plant growth and tissue collections, Sue Cambron (USDA-ARS) for maintaining Hessian fly stocks, and Alisha Johnson (USDA-ARS) for assistance with phylogenetic analysis. Mention of a commercial or a proprietary product does not constitute endorsement or recommendation for its use by the USDA. NR 75 TC 2 Z9 2 U1 5 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JAN 16 PY 2015 VL 15 AR 3 DI 10.1186/s12870-014-0396-y PG 16 WC Plant Sciences SC Plant Sciences GA CA0JO UT WOS:000348604400003 PM 25592131 ER PT J AU Yun, J Fan, XT Li, XH Jin, TZ Jia, XY Mattheis, JP AF Yun, Juan Fan, Xuetong Li, Xihong Jin, Tony Z. Jia, Xiaoyu Mattheis, James P. TI Natural surface coating to inactivate Salmonella enterica serovar Typhimurium and maintain quality of cherry tomatoes SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Zein; Salmonella; Essential oil; SEM; Firmness; Color ID ESCHERICHIA-COLI O157/H7; ESSENTIAL OILS; FRESH PRODUCE; ZEIN FILMS; SHELF-LIFE; ANTIMICROBIAL PROPERTIES; UNITED-STATES; RAW TOMATOES; CINNAMON OIL; FRUIT AB The objective of the present study was to investigate the effectiveness of zein-based coatings in reducing populations of Salmonella enterica serovar Typhimurium and preserving quality of cherry tomatoes. Tomatoes were inoculated with a cocktail of S. Typhimurium LT2 plus three attenuated strains on the smooth skin surface and stem scar area. The zein-based coatings with and without cinnamon (up to 20%) and mustard essential oil or a commercial wax formulation were applied onto tomatoes and the treated fruits were stored at 10 degrees C for up to 3 weeks. Populations of S. Typhimurium decreased with increased essential oil concentration and storage duration. S. Typhimurium populations on the smooth skin surface were reduced by 4.6 and 2.8 log colony forming units (CFU)/g by the zein coatings with 20% cinnamon and 20% mustard oil, respectively, 5 h after coating. The same coating reduced populations of S. Typhimurium to levels below detection limit (1.0 log CFU/g) on the stem scar area of tomato during 7 days of storage at 10 degrees C. Salmonella populations were not reduced on fruit coated with the commercial wax. All of the coatings resulted in reduced weight loss compared with uncoated control. Compared with the control, loss of firmness and ascorbic acid during storage was prevented by all of the coatings except the zein coating with 20% mustard oil which enhanced softening. Color was not consistently affected by any of the coating treatments during 21 days of storage at 10 degrees C. The results suggest that the zein-based coating containing cinnamon oil might be used to enhance microbial safety and quality of tomato. Published by Elsevier B.V. C1 [Yun, Juan; Li, Xihong; Jia, Xiaoyu] Tianjin Univ Sci & Technol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China. [Fan, Xuetong; Jin, Tony Z.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Yun, Juan] Tianjin Gasin Donghui Preservat Technol Co Ltd, Tianjin 300457, Peoples R China. [Mattheis, James P.] ARS, USDA, Tree Fruit Res Lab, Wyndmoor, PA 19038 USA. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov RI Yun, Juan/E-4164-2016; OI Jin, Tony/0000-0003-0504-5817 NR 71 TC 8 Z9 8 U1 9 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JAN 16 PY 2015 VL 193 BP 59 EP 67 DI 10.1016/j.iffoodmicro.2014.10.013 PG 9 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AY3WP UT WOS:000347510600009 PM 25462924 ER PT J AU Franz, E Delaquis, P Morabito, S Beutin, L Gobius, K Rasko, DA JimBono NigelFrench Osek, J Lindstedt, BA Muniesa, M Manning, S LeJeune, J Callaway, T Beatson, S Eppinger, M Dallman, T Forbes, KJ Aarts, H Pearl, DL Gannon, VPJ Laing, CR Strachan, NJC AF Franz, Eelco Delaquis, Pascal Morabito, Stefano Beutin, Lothar Gobius, Kari Rasko, David A. JimBono NigelFrench Osek, Jacek Lindstedt, Bjorn-Arne Muniesa, Maite Manning, Shannon LeJeune, Jeff Callaway, Todd Beatson, Scott Eppinger, Mark Dallman, Tim Forbes, Ken J. Aarts, Henk Pearl, David L. Gannon, Victor P. J. Laing, Chad R. Strachan, Norval J. C. TI Exploiting the explosion of information associated with whole genome sequencing to tackle Shiga toxin-producing Escherichia coli (STEC) in global food production systems (vol 187, pg 57, 2014) SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Correction C1 [Franz, Eelco; Aarts, Henk] Natl Inst Publ Hlth & Environm, NL-3720 BA Bilthoven, Netherlands. [Delaquis, Pascal] Agr & Agri Food Canada, Summerland, BC, Canada. [Morabito, Stefano] Natl Inst Hlth, Rome, Italy. [Beutin, Lothar] Fed Inst Risk Assessment, Berlin, Germany. [Gobius, Kari] CSIRO, Archerfield Bc, Qld, Australia. [Rasko, David A.] Univ MD Sch Med, Baltimore, MD USA. [JimBono] USDA ARS, Clay Ctr, NE USA. [NigelFrench] Massey Univ, Inst Vet Anim & Biomed Sci, Infect Dis Res Ctr, EpiLab, Palmerston North, New Zealand. [Osek, Jacek] Natl Inst Vet Res, Pulawy, Poland. [Lindstedt, Bjorn-Arne] Akershus Univ Hosp Lorenskog, Unit Gene Technol, Lorenskog, Norway. [Muniesa, Maite] Univ Barcelona, Dept Microbiol, Barcelona, Spain. [Manning, Shannon] MI State Univ, E Lansing, MI USA. [LeJeune, Jeff] Ohio State Univ, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Callaway, Todd] USDA ARS, College Stn, TX USA. [Beatson, Scott] Univ Queensland, Australian Infect Dis Res Ctr, St Lucia, Qld, Australia. [Eppinger, Mark] Univ Texas San Antonio, South Texas Ctr Emerging Infect Dis, Dept Biol, San Antonio, TX USA. [Dallman, Tim] Hlth Protect Agcy, London, England. [Forbes, Ken J.] Univ Aberdeen, Sch Med & Dent, Aberdeen, Scotland. [Pearl, David L.] Univ Guelph, Ontario Vet Coll, Guelph, ON, Canada. [Gannon, Victor P. J.; Laing, Chad R.] Publ Hlth Agcy Canada, Lab Foodborne Zoonoses, Lethbridge, AB, Canada. [Strachan, Norval J. C.] Univ Aberdeen, Sch Biol Sci, Aberdeen, Scotland. RP Strachan, NJC (reprint author), Univ Aberdeen, Sch Biol Sci, Aberdeen, Scotland. EM n.strachan@abdn.ac.uk RI Gobius, Kari/I-4700-2013 NR 1 TC 0 Z9 0 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JAN 16 PY 2015 VL 193 BP 159 EP 159 DI 10.1016/j.ijfoodmicro.2014.09.026 PG 1 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AY3WP UT WOS:000347510600021 ER PT J AU Osadebe, LU Manthiram, K McCollum, AM Li, Y Emerson, GL Gallardo-Romero, NF Doty, JB Wilkins, K Zhao, H Drew, CP Metcalfe, MG Goldsmith, CS Muehlenbachs, A Googe, PB Dunn, J Duenckel, T Henderson, H Carroll, DS Zaki, SR Denison, MR Reynolds, MG Damon, IK AF Osadebe, Lynda U. Manthiram, Kalpana McCollum, Andrea M. Li, Yu Emerson, Ginny L. Gallardo-Romero, Nadia F. Doty, Jeffrey B. Wilkins, Kimberly Zhao, Hui Drew, Clifton P. Metcalfe, Maureen G. Goldsmith, Cynthia S. Muehlenbachs, Atis Googe, Paul B. Dunn, John Duenckel, Todd Henderson, Heather Carroll, Darin S. Zaki, Sherif R. Denison, Mark R. Reynolds, Mary G. Damon, Inger K. TI Novel Poxvirus Infection in 2 Patients From the United States SO CLINICAL INFECTIOUS DISEASES LA English DT Article DE poxvirus; skin infection; parapoxvirus; immunocompromised; imiquimod ID ORF VIRUS-INFECTION; GENUS PARAPOXVIRUS; RECURRENT ORF; IMIQUIMOD; GIANT; DEER AB Background. Some human poxvirus infections can be acquired through zoonotic transmission. We report a previously unknown poxvirus infection in 2 patients, 1 of whom was immunocompromised; both patients had known equine contact. Methods. The patients were interviewed and clinical information was abstracted from the patients' medical files. Biopsies of the skin lesions were collected from both patients for histopathology, immunohistochemistry, and transmission electron microscopy analysis. Oral and skin swabs were collected from animals with frequent contact with the patients, and environmental sampling including rodent trapping was performed on the farm where the immunosuppressed patient was employed. "Pan-pox and high Guanine-cytosine" polymerase chain reaction assays were performed on patient, animal, and environmental isolates. Amplicon sequences of the viral DNA were used for agent identification and phylogenetic analysis. Results. Specimens from both human cases revealed a novel poxvirus. The agent shares 88% similarity to viruses in the Parapoxvirus genus and 78% to those in the Molluscipoxvirus genus but is sufficiently divergent to resist classification as either. All animal and environmental specimens were negative for poxvirus and both patients had complete resolution of lesions. Conclusions. This report serves as a reminder that poxviruses should be considered in cutaneous human infections, especially in individuals with known barnyard exposures. The clinical course of the patients was similar to that of parapoxvirus infections, and the source of this virus is currently unknown but is presumed to be zoonotic. This report also demonstrates the importance of a comprehensive approach to diagnosis of human infections caused by previously unknown pathogens. C1 [Osadebe, Lynda U.] Ctr Dis Control & Prevent, Epidem Intelligence Serv, Div Sci Educ & Profess Dev, Program Off, Atlanta, GA 30333 USA. [Osadebe, Lynda U.; McCollum, Andrea M.; Li, Yu; Emerson, Ginny L.; Gallardo-Romero, Nadia F.; Doty, Jeffrey B.; Wilkins, Kimberly; Zhao, Hui; Drew, Clifton P.; Metcalfe, Maureen G.; Goldsmith, Cynthia S.; Muehlenbachs, Atis; Carroll, Darin S.; Zaki, Sherif R.; Reynolds, Mary G.; Damon, Inger K.] Ctr Dis Control & Prevent, Div High Consequence Pathogens & Pathol, Atlanta, GA 30333 USA. [Googe, Paul B.] Vanderbilt Univ, Med Ctr, Knoxville Dermatopathol Lab, Nashville, TN USA. [Manthiram, Kalpana] Vanderbilt Univ, Med Ctr, Div Pediat Infect Dis, Nashville, TN USA. [Dunn, John; Henderson, Heather] Tennessee Dept Hlth, Nashville, TN USA. [Duenckel, Todd] US Anim & Plant Hlth Inspect Serv, USDA, Reg Off, Nashville, TN USA. RP Reynolds, MG (reprint author), Ctr Dis Control & Prevent, Poxvirus & Rabies Branch, 1600 Clifton Rd NE,MS A30, Atlanta, GA 30333 USA. EM nzr6@cdc.gov OI Googe, Paul/0000-0002-9185-3548 NR 18 TC 5 Z9 5 U1 0 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD JAN 15 PY 2015 VL 60 IS 2 BP 195 EP 202 DI 10.1093/cid/ciu790 PG 8 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA CB6TR UT WOS:000349760100004 PM 25301210 ER EF