FN Thomson Reuters Web of Science™ VR 1.0 PT J AU van Riper, CJ Wallen, KE Landon, AC Petriello, MA Kyle, GT Absher, J AF van Riper, Carena J. Wallen, Kenneth E. Landon, Adam C. Petriello, Michael A. Kyle, Gerard T. Absher, James TI Modeling the trust-risk relationship in a wildland recreation setting: A social exchange perspective SO JOURNAL OF OUTDOOR RECREATION AND TOURISM-RESEARCH PLANNING AND MANAGEMENT LA English DT Article DE Trust; Risk; Recreation; Social exchange; Structural equation modeling ID NATURAL-RESOURCE MANAGEMENT; SALIENT VALUE SIMILARITY; INTEGRATIVE MODEL; ORGANIZATIONAL TRUST; PLACE ATTACHMENT; FIT INDEXES; TRUSTWORTHINESS; INVOLVEMENT; PERCEPTION; PERFORMANCE AB We empirically tested relationships among the characteristics of trustworthiness, trust instilled in river guides, and risks perceived by whitewater recreationists that rafted a Wild and Scenic River in the Southern Sierra Nevada, CA. Drawing on a social exchange framework, we used survey data to address the following objectives: 1) investigate three dimensions of trustworthiness, including ability, integrity, and benevolence; 2) examine trust in decisions and trust in values that recreationists associated with their river guides; and 3) determine the effects of trustworthiness and trust on recreationists' beliefs that river guides minimized psychological and social risks from rafting the Kern River. Results from a latent variable path model revealed that the ability and integrity of river guides played important roles in explaining why they were trusted by recreationists, which in turn positively influenced the extent to which guides were believed to minimize risk. Contrary to previous research, we found that trust in values did not play a substantive role in predicting risk perception. A greater understanding of the trust-risk relationship will shed light on how public land management agencies can effectively navigate risk in dangerous wildland environments and provide access to otherwise inaccessible resources owned and valued by the public. MANAGEMENT IMPLICATIONS Our results offer insight on how public land management agencies can negotiate risk and maintain high quality recreational opportunities afforded by wildland environments. Specifically, our study findings suggest: Whitewater recreationists may not be able to access and/or enjoy wildland environments without trust and trustworthiness garnered from the assistance of guides. The trust-risk relationship can be understood from a social exchange perspective. Trustworthiness is an important mechanism for explaining trust in decisions and trust in values. The extent to which river guides are thought to minimize risks can be predicted by the ability and integrity of a river guide and trust placed on their decisions. The benevolence of river guides does not factor into the formation of trust or the risk perceptions of whitewater recreationists. (C) 2016 Elsevier Ltd. All rights reserved. C1 [van Riper, Carena J.] Univ Illinois, Pk & Environm Behav Res Grp, Dept Recreat Sport & Tourism, 1206 South Fourth St, Champaign, IL 61820 USA. [Wallen, Kenneth E.; Petriello, Michael A.; Kyle, Gerard T.] Texas A&M Univ, Human Dimens Nat Resources Lab, Dept Recreat Pk & Tourism Sci, 2261 TAMU 600 John Kimbrough Blvd, College Stn, TX 77843 USA. [Wallen, Kenneth E.; Petriello, Michael A.] Texas A&M Univ, NSF IGERT Appl Biodivers Sci Program, 110G Old Heep Bldg, College Stn, TX 77843 USA. [Absher, James] US Forest Serv, USDA, Emeritus 46687 Veater Ranch Rd, Coarsegold, CA 93614 USA. [Landon, Adam C.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP van Riper, CJ (reprint author), Univ Illinois, Pk & Environm Behav Res Grp, Dept Recreat Sport & Tourism, 1206 South Fourth St, Champaign, IL 61820 USA. EM cvanripe@illinois.edu NR 89 TC 2 Z9 2 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2213-0780 EI 2213-0799 J9 J OUTDO RECREAT TOUR JI J. Outdo. Recreat. Tour. Res. Plan. PD APR PY 2016 VL 13 BP 23 EP 33 DI 10.1016/j.jort.2016.03.001 PG 11 WC Hospitality, Leisure, Sport & Tourism SC Social Sciences - Other Topics GA DN0NP UT WOS:000376760900004 ER PT J AU Wright, NC Saag, KG Dawson-Hughes, B Khosla, S Siris, ES AF Wright, N. C. Saag, K. G. Dawson-Hughes, B. Khosla, S. Siris, E. S. TI THE IMPACT OF THE NATIONAL BONE HEALTH ALLIANCE (NBHA) DIAGNOSTIC CRITERIA ON THE PREVALENCE OF OSTEOPOROSIS IN THE UNITED STATES SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT WCO-IOF-ESCEO World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases CY APR 14-17, 2016 CL Malaga, SPAIN SP WCO, IOF, ESCEO C1 [Wright, N. C.] Univ Alabama, Dept Epidemiol, Birmingham, W Midlands, England. [Saag, K. G.] Univ Alabama, Div Clin Immunol & Rheumatol, Birmingham, W Midlands, England. [Dawson-Hughes, B.] Tufts Univ, USDA, Nutr Res Ctr, Boston, MA 02111 USA. [Khosla, S.] Mayo Clin, Div Endocrinol Metab Diabet Nutr & Internal Med, Rochester, MN USA. [Siris, E. S.] Columbia Univ, Coll Phys & Surg, Dept Med, New York, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD APR PY 2016 VL 27 SU 1 MA P527 BP S295 EP S296 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DM6NH UT WOS:000376469102047 ER PT J AU Chaboo, CS Chamorro, ML Scholler, M AF Chaboo, Caroline S. Chamorro, Maria Lourdes Schoeller, Matthias TI CATALOGUE OF KNOWN IMMATURE STAGES OF CAMPTOSOMATE LEAF BEETLES (COLEOPTERA, CHRYSOMELIDAE, CRYPTOCEPHALINAE AND LAMPROSOMATINAE) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Morphology; egg; larva; pupa; myrmecophily; Camptosomata; life history ID ANOMOEA-FLAVOKANSIENSIS COLEOPTERA; 1ST INSTAR LARVA; LIFE-HISTORY; CHLAMISUS CRIBRIPENNIS; NEOCHLAMISUS KARREN; 1ST-INSTAR LARVA; HOST-SPECIFICITY; NATURAL-HISTORY; CADMUS ERICHSON; EGG BURSTERS AB Lack of syntheses of knowledge on immature stages of insects impedes accurate understanding of their diversity, biology and evolution. Literature describing known immature stages of case-bearing chrysomelids is catalogued, i.e., 358 species of Cryptocephalinae Gyllenhal and 13 species of Lamprosomatinae Lacordaire. The catalogue covers the world fauna including fossils, and reviews information on eggs, larvae, pupae, host plants, distribution, host ants (for myrmecophiles), and parasitoids and predators. This synthesis should stimulate efforts to discover and study immatures, and generate new hypotheses about the evolution in these two sister clades of leaf beetles. C1 [Chaboo, Caroline S.] Univ Kansas, Div Entomol, 1501 Crestline Dr,Suite 140, Lawrence, KS 66045 USA. [Chamorro, Maria Lourdes] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, MRC 168,POB 37012, Washington, DC 20013 USA. [Schoeller, Matthias] Humboldt Univ, Fac Life Sci, Lentzeallee 55-57, D-14195 Berlin, Germany. RP Chaboo, CS (reprint author), Univ Kansas, Div Entomol, 1501 Crestline Dr,Suite 140, Lawrence, KS 66045 USA. EM cschaboo@ku.edu FU NSF-EPSCOR [66928]; U.S. Department of Agriculture (U.S.D.A.) FX This research was supported by the authors' respective institutions and NSF-EPSCOR grant #66928 to C.S. Chaboo. The research of Lourdes Chamorro is supported by the U.S. Department of Agriculture (U.S.D.A.); USDA is an equal opportunity provider and employer. In this project, assembling literature was a challenge so we are grateful to the following for helping us track down certain works: Alexander Konstantinov and Vladimir Blagoderov for part of the Russian literature and translations; Dashdondog Narangarvuu for part of the literature on Mongolian species; Davide Sassi and Stefano Zoia for part of Italian literature, and Michael Schmitt for part of the German literature. We are indebted to the following reviewers: Federico A. Agrain, Patrice Bouchard, Matthew Buffington, Vivian Flinte, Douglas Yanega, three anonymous reviewers, and the managing editors of the journal who provided valuable comments on the final version of the manuscript. NR 490 TC 2 Z9 2 U1 0 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2016 VL 118 IS 2 BP 150 EP 217 DI 10.4289/0013-8797.118.1.150 PG 68 WC Entomology SC Entomology GA DN0NH UT WOS:000376760100002 ER PT J AU Chamorro, ML Persson, J Torres-Santana, CW Keularts, J Scheffer, SJ Lewis, ML AF Chamorro, M. Lourdes Persson, Joshua Torres-Santana, Christian W. Keularts, Jeff Scheffer, Sonja J. Lewis, Matthew L. TI MOLECULAR AND MORPHOLOGICAL TOOLS TO DISTINGUISH SCYPHOPHORUS ACUPUNCTATUS GYLLENHAL, 1838 (CURCULIONIDAE: DRYOPHTHORINAE): A NEW WEEVIL PEST OF THE ENDANGERED CENTURY PLANT, AGAVE EGGERSIANA FROM ST. CROIX, US VIRGIN ISLANDS SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE larval morphology; Palm weevils; COI; EF1a; conservation; Endangered Species Act; West Indies; Scyphophorus yuccae; Sphenophorus cubensis ID BAYESIAN PHYLOGENETIC INFERENCE; COLEOPTERA-CURCULIONIDAE; LARVAL; CHARACTERS; MRBAYES; HOST AB The agave snout weevil (AGW) or sisal weevil, Scyphophorus acupunctatus Gyllenhal is here reported for the first time in St. Croix, U.S. Virgin Islands (USVI) where it threatens Agave eggersiana Trel., a USVI endemic and endangered century-plant. We provide molecular, morphological, and behavioral characters to successfully distinguish the two known Scyphophorus species at all developmental stages. We identified seven new larval characters on the mandibles and characters relating to the chaetotaxy of the labrum and labio-maxillary complex as well as new, putatively informative characters for weevil systematics: chitinized arm of mentum (postlabial strut or postlabial bracon) and the presence of 4 ventral malar setae, instead of 5. In the pupae, the difference in number and placement of rostral setae were also found to be diagnostic. We analyzed two genes, mtCO1 and EF1a, to confirm the identity of the immatures. Phylogenetic analysis of both genes separately and together suggests a clear pattern of substantial phylogeographic structure with specimens clustering by geographic location and this pattern strongly suggests the presence of cryptic species or allopatrically diverged populations. We provide management recommendations for the protection of Agave eggersiana against the threat posed by ASW. We also report, for the first time, the presence of Sphenophorus cubensis (Buchanan) in St. Croix and Scyphophorus yuccae Horn in Panama on Hesperoyucca whipplei (Torr.) Trel. (Asparagaceae: Agavoideae). C1 [Chamorro, M. Lourdes] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. [Persson, Joshua] George Mason Univ, Fairfax, VA 22030 USA. [Torres-Santana, Christian W.] US Forest Serv, Int Inst Trop Forestry, USDA, 1201 Calle Ceiba, San Juan, PR 00926 USA. [Torres-Santana, Christian W.] Fdn Luis Munoz Marin, Arboretum Parque Dona Ines, RR 2,Buzon 5, San Juan, PR 00926 USA. [Keularts, Jeff] Univ Virgin Isl, St Croix Campus,RR1 Box 10000, Kingshill, VI 00850 USA. [Scheffer, Sonja J.] ARS, Systemat Entomol Lab, USDA, Beltsville, MD USA. RP Chamorro, ML (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM lourdes.chamorro@ars.usda.gov NR 58 TC 0 Z9 0 U1 3 U2 4 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2016 VL 118 IS 2 BP 218 EP 243 DI 10.4289/0013-8797.118.2.218 PG 26 WC Entomology SC Entomology GA DN0NH UT WOS:000376760100003 ER PT J AU Kula, RR AF Kula, Robert R. TI CARDIOCHILINAE AND ICHNEUTINAE (HYMENOPTERA: BRACONIDAE) OF KONZA PRAIRIE SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE biodiversity; conservation; Flint Hills; gallery forest; Great Plains; Kansas; Nearctic; new species; parasitoid; tallgrass prairie; taxonomy ID WASPS HYMENOPTERA; GENERA; ICHNEUMONOIDEA; REVISION; HALIDAY; TRIBE; RNA AB The results of a survey of Cardiochilinae and Ichneutinae (Hymenoptera: Braconidae) at Konza Prairie near Manhattan, Kansas are reported. Eleven sites representing prairie and woodland/wetland areas, including gallery forest, were sampled in 2001 and 2005 using Malaise traps and a canopy trap. Selected sites were also sampled haphazardly using pan traps and a sweep net. Six species of Cardiochilinae and two species of Ichneutinae were collected. Cardiochilines were collected at woodland/wetland sites only except a single specimen collected at a prairie site. Both species of Ichneutinae were collected at woodland/wetland sites and prairie sites. Cardiochiles floridanus (Ashmead) (n=3) and Cardiochiles minutus (Cresson) (n=4) were the most abundant species of Cardiochilinae in 2001 and 2005, respectively. Paroligoneurus newharti Kula, new species, the most abundant species of Ichneutinae in 2001 and only ichneutine collected in 2005, is described. Cardiochiles insculptus Mao and Cardiochiles minutus (Cresson) are reported from Kansas for the first time. C1 [Kula, Robert R.] ARS, Systemat Entomol Lab, Beltsville Agr Res Ctr, USDA,Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. RP Kula, RR (reprint author), ARS, Systemat Entomol Lab, Beltsville Agr Res Ctr, USDA,Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. EM Robert.Kula@ars.usda.gov NR 45 TC 0 Z9 0 U1 1 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2016 VL 118 IS 2 BP 273 EP 288 DI 10.4289/0013-8797.118.2.273 PG 16 WC Entomology SC Entomology GA DN0NH UT WOS:000376760100007 ER PT J AU Miller, GL Jensen, AS Favret, C Metz, MA Parmenter, RR AF Miller, Gary L. Jensen, Andrew S. Favret, Colin Metz, Mark A. Parmenter, Robert R. TI THE FIRST REPORT OF THE APHIDS (HEMIPTERA: STERNORRHYNCHA: APHIDIDAE) OF THE VALLES CALDERA NATIONAL PRESERVE, NEW MEXICO, USA SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Hemiptera; Aphidomorpha; Aphididae ID APHIDOIDEA; CHECKLIST; AMERICA; NORTH AB We report 39 species, belonging to 22 genera of aphids (Hemiptera: Aphididae), collected within and near the boundary of the Valles Caldera National Preserve in the Jemez Mountains of northern New Mexico, USA. Of these, 26 (67%) represent new distribution records for New Mexico. Plant genera/species from which the specimens were collected also are listed when available. C1 [Miller, Gary L.; Metz, Mark A.] ARS, USDA, Systemat Entomol Lab, Beltsville, MD USA. [Jensen, Andrew S.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Favret, Colin] Univ Montreal, Dept Biol Sci, Biodivers Ctr, Montreal, PQ H3C 3J7, Canada. [Parmenter, Robert R.] Natl Pk Serv, Valles Caldera Natl Preserve, Sci & Resource Stewardship Div, Jemez Springs, NM USA. RP Miller, GL (reprint author), ARS, USDA, Systemat Entomol Lab, Beltsville, MD USA. RI Favret, Colin/F-7726-2012 OI Favret, Colin/0000-0001-6243-3184 NR 23 TC 0 Z9 0 U1 1 U2 1 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2016 VL 118 IS 2 BP 289 EP 296 DI 10.4289/0013-8797.118.2.289 PG 8 WC Entomology SC Entomology GA DN0NH UT WOS:000376760100008 ER PT J AU Cole, JB da Silva, MVGB AF Cole, John Bruce Gualberto Barbosa da Silva, Marcos Vinicius TI Genomic selection in multi-breed dairy cattle populations SO REVISTA BRASILEIRA DE ZOOTECNIA-BRAZILIAN JOURNAL OF ANIMAL SCIENCE LA English DT Review DE crossbred cattle; crossbreeding; genomic evaluation; multi-breed populations ID GENETIC EVALUATION; CROSSBRED PERFORMANCE; RELATIONSHIP MATRIX; FULL PEDIGREE; BEEF-CATTLE; BROWN SWISS; INFORMATION; ACCURACY; VALUES; PREDICTIONS AB Genomic selection has been a valuable tool for increasing the rate of genetic improvement in purebred dairy cattle populations. However, there also are many large populations of crossbred dairy cattle in the world, and multi-breed genomic evaluations may be a valuable tool for improving rates of genetic gain in those populations. Multi-breed models are an extension of single-breed genomic models in which a genomic relationship matrix is used to account for the breed origin of alleles in the population, as well as allele frequency differences between breeds. Most studies have found little benefit from multi-breed evaluations for pure breeds that have large reference populations. However, breeds with small reference populations may benefit from inclusion in a multi-breed evaluation without adversely affecting evaluations for purebred performance. Most research has been conducted in taurine breeds, so additional research is needed to determine the value of multi-breed reference populations for composite and synthetic breeds that include both indicine and taurine cattle adapted to tropical climates. C1 [Cole, John Bruce] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD USA. [Gualberto Barbosa da Silva, Marcos Vinicius] Embrapa Gado de Leite, Juiz De Fora, MG, Brazil. RP Cole, JB (reprint author), ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD USA. EM john.cole@ars.usda.gov RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [CNPq - 301025/2014-2]; Agricultural Research Service of the United States Department of Agriculture [1265-31000-096-00] FX The authors thank Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq - 301025/2014-2) for the financial support. Cole was supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information", of the Agricultural Research Service of the United States Department of Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 62 TC 0 Z9 0 U1 5 U2 10 PU REVISTA BRASILEIRA ZOOTECNIA BRAZILIAN JOURNAL ANIMAL SCI PI VICOSA-MG PA UNIVERSIDADE FEDERAL VICOSA,, 36571-000 VICOSA-MG, BRAZIL SN 1806-9290 J9 REV BRAS ZOOTECN JI Rev. Bras. Zootecn. PD APR PY 2016 VL 45 IS 4 BP 195 EP 202 DI 10.1590/S1806-92902016000400008 PG 8 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA DN4FO UT WOS:000377019100008 ER PT J AU Gu, HM Bergman, R AF Gu, Hongmei Bergman, Richard TI LIFE-CYCLE ASSESSMENT OF A DISTRIBUTED-SCALE THERMOCHEMICAL BIOENERGY CONVERSION SYSTEM SO WOOD AND FIBER SCIENCE LA English DT Article DE Thermochemical pyrolysis conversion; syngas; biochar; woody biomass; life-cycle inventory (LCI); life-cycle assessment (LCA) ID EMISSIONS; PYROLYSIS; BIOCHAR; BIOMASS; IMPACTS; LIQUID AB Expanding bioenergy production from woody biomass has the potential to decrease net greenhouse gas (GHG) emissions and improve the energy security of the United States. Science-based and internationally accepted life-cycle assessment (LCA) is an effective tool for policy makers to make scientifically informed decisions on expanding renewable energy production from newly developed bioenergy technologies. A distributed-scale high-temperature thermochemical conversion system, referred to as the Tucker renewable natural gas (RNG) unit, was evaluated for producing medium-energy synthesis gas (syngas) and biochar along with its waste from harvested woody biomass. Mass and energy balances, cumulative energy demand, and life-cycle inventory (LCI) flows were calculated based on operational data from a 1-h continuous run. Emissions data summarized from the cradle-to-gate LCI showed biomass and fossil CO2 emissions of 0.159 and 0.534 kg, respectively, for each oven-dry (OD) kilogram of wood chips pyrolyzed. LCA, applied in accordance with International Organization for Standardization standards, was used to determine the potential environmental impacts. Total GHG was 0.595 kg CO2 eq per OD kilogram of wood chips processed. Contributions to total GHG were 20.7% from upstream forest resource extraction and chip processing at sawmills and 77.6% from the thermochemical conversion process with propane combustion. The remaining 1.62% was from parasitic electricity operating the Tucker RNG unit. Quantifying global warming showed the carbon benefits (eg, low GHG emissions) along with the carbon hotspots from burning propane to maintain the endothermic reaction in the Tucker RNG unit. The use of low-energy syngas generated from what was originally a waste in the pyrolysis reaction to augment propane combustion would decrease GHG emissions (ie, fossil CO2) by about 30.4%. C1 [Bergman, Richard] USDA Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI USA. EM hongmeigu@fs.fed.us; rbergman@fs.fed.us RI 张, 志芳/P-1635-2016 OI 张, 志芳/0000-0001-8313-9240 FU Biomass Research and Development Initiative, competitive from the USDA National Institute of Food and Agriculture [2011-10006-30357]; Agriculture and Food Research Initiative FX This project was supported by the Agriculture and Food Research Initiative, Biomass Research and Development Initiative, competitive grant no. 2011-10006-30357 from the USDA National Institute of Food and Agriculture. NR 29 TC 0 Z9 0 U1 5 U2 7 PU SOC WOOD SCI TECHNOL PI MADISON PA ONE GIFFORD PINCHOT DR, MADISON, WI 53705 USA SN 0735-6161 J9 WOOD FIBER SCI JI Wood Fiber Sci. PD APR PY 2016 VL 48 IS 2 BP 129 EP 141 PG 13 WC Forestry; Materials Science, Paper & Wood; Materials Science, Textiles SC Forestry; Materials Science GA DN4SM UT WOS:000377057500007 ER PT J AU Mott, IW Cook, D Lee, ST Stonecipher, CA Panter, KE AF Mott, Ivan W. Cook, Daniel Lee, Stephen T. Stonecipher, Clinton A. Panter, Kip E. TI Phylogenetic examination of two chemotypes of Lupinus leucophyllus SO BIOCHEMICAL SYSTEMATICS AND ECOLOGY LA English DT Article DE Lupinus leucophyllus; Lupinus polyphyllus; Lupine; Alkaloid; Cladogram; Genetic diversity ID CROOKED CALF DISEASE; ALKALOID PROFILES; GENETIC DIVERSITY; QUINOLIZIDINE ALKALOIDS; ARGENTEUS; AFLP; LEGUMINOSAE; SEQUENCES; FABACEAE; FORMOSUS AB Lupines (Lupinus spp.) are a common legume found on western U.S. rangelands. Lupinus spp. may contain quinolizidine and/or piperidine alkaloids that can be toxic and/or teratogenic to grazing livestock. Lupinus leucophyllus and Lupinus polyphyllus in particular represent important species in the rangelands of southeast Washington and northeast Oregon. The objectives of this study were to identify the alkaloid profiles of these two species, and to explore the phylogenetic relationship among the different populations using amplified fragment length polymorphism (AFLP) markers. Two chemotypes were found among the 14 accessions of Lupinus leucophyllyus. Chemotype A contained anagyrine, a potent teratogen in cattle, and thus would pose a risk to cause crooked calf syndrome in grazing cattle, while Chemotype B did not contain anagyrine and poses no teratogenic risk. No alkaloids were detected in the L. polyphyllus plants collected in this area. Phylogenetic analysis showed that L leucophyllus accessions with the same chemotype that were geographically proximal were closely grouped in the cladogram; however, accessions that were geographically proximal that represented different chemotypes did not cluster together. Taken together, these results suggest that for the studied species, chemotype is the principle factor in determining relatedness followed by geography. Published by Elsevier Ltd. C1 [Mott, Ivan W.] ARS, USDA, Forage & Range Res, 690 N 1100 E, Logan, UT 84322 USA. [Cook, Daniel; Lee, Stephen T.; Stonecipher, Clinton A.; Panter, Kip E.] ARS, USDA, Poisonous Plant Res, 1150 E 1400 N, Logan, UT 84341 USA. RP Mott, IW (reprint author), ARS, USDA, Forage & Range Res, 690 N 1100 E, Logan, UT 84322 USA. EM ivan.mott@ars.usda.gov NR 39 TC 0 Z9 0 U1 3 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0305-1978 EI 1873-2925 J9 BIOCHEM SYST ECOL JI Biochem. Syst. Ecol. PD APR PY 2016 VL 65 BP 57 EP 65 DI 10.1016/j.bse.2016.02.004 PG 9 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DK0JN UT WOS:000374599200008 ER PT J AU Cook, D Slominski, A Gardner, DR Pfister, JA Irwin, RE AF Cook, Daniel Slominski, Anthony Gardner, Dale R. Pfister, James A. Irwin, Rebecca E. TI Seasonal variation in the secondary chemistry of foliar and reproductive tissues of Delphinium nuttallianum SO BIOCHEMICAL SYSTEMATICS AND ECOLOGY LA English DT Article DE Larkspur; Delphinium; Norditerpene alkaloids; Plant parts; Life history ID TOXIC NORDITERPENOID ALKALOIDS; RESOURCE AVAILABILITY; SALIX-SERICEA; LARKSPUR; BARBEYI; SPP.; PATTERNS; CATTLE; OCCIDENTALE; DEFENSE AB Plant secondary compounds are critical in affecting interactions between plants and their herbivores. The norditerpene alkaloids are secondary compounds in Delphinium (larkspur) species which are divided into two classes: the N-(methylsuccinimido) anthranoylly-coctonine (MSAL-type) and non MSAL-type, and are known to be toxic to herbivorous insects and livestock. Alkaloid concentrations were measured in a whole plant context in vegetative and reproductive tissues in Delphinium nuttallianum at different stages of plant maturity at two locations to explore how plant maturity affected alkaloid concentrations within a growing season. Alkaloid concentrations differed between vegetative and reproductive tissues, with vegetative tissues having significantly lower alkaloid concentrations than reproductive tissues. However, no systematic differences in alkaloid concentrations were observed at different plant maturity stages across the growing season. Based on the data we suggest that alkaloid allocation in different plant parts of D. nuttallianum is influenced by life history of the plant, consistent with plant defense theory. At one location, as pods mature the qualitative alkaloid composition changed through structural diversification of the alkaloids present. The ecological significance of this structural diversification awaits further exploration. Published by Elsevier Ltd. C1 [Cook, Daniel; Gardner, Dale R.; Pfister, James A.] ARS, USDA, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. [Slominski, Anthony; Irwin, Rebecca E.] Rocky Mt Biol Labs, POB 519, Crested Butte, CO 81224 USA. [Irwin, Rebecca E.] N Carolina State Univ, Dept Appl Ecol, Raleigh, NC 27695 USA. [Slominski, Anthony] Montana State Univ, Dept Ecol, Bozeman, MT 59715 USA. RP Cook, D (reprint author), ARS, USDA, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA.; Irwin, RE (reprint author), N Carolina State Univ, Dept Appl Ecol, Raleigh, NC 27695 USA. EM daniel.cook@ars.usda.gov; reirwin@ncsu.edu FU National Science Foundation [DEB-0841862] FX We wish to thank Jessie Roper for technical assistance. Field assistance and laboratory access at the Rocky Mountain Biological Laboratory were provided by a grant from the National Science Foundation (DEB-0841862). NR 47 TC 0 Z9 0 U1 6 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0305-1978 EI 1873-2925 J9 BIOCHEM SYST ECOL JI Biochem. Syst. Ecol. PD APR PY 2016 VL 65 BP 93 EP 99 DI 10.1016/j.bse.2016.02.006 PG 7 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DK0JN UT WOS:000374599200013 ER PT J AU Estell, RE Fredrickson, EL James, DK AF Estell, Rick E. Fredrickson, Ed L. James, Darren K. TI Effect of light intensity and wavelength on concentration of plant secondary metabolites in the leaves of Flourensia cernua SO BIOCHEMICAL SYSTEMATICS AND ECOLOGY LA English DT Article DE Flourensia cernua; Plant secondary metabolites; Phenolics; Shade; Terpenes; Ultraviolet light ID ULTRAVIOLET-B RADIATION; UV-B; LEAF SURFACE; CHEMICAL-COMPOSITION; VOLATILE COMPOUNDS; INSECT HERBIVORY; DWARF SHRUBS; ELEVATED CO2; AVAILABILITY; CHEMISTRY AB Flourensia cernua (tarbush) is a shrub that has encroached into grasslands in many areas of the northern Chihuahuan Desert and contains high levels of carbon-based secondary compounds. Concentrations of secondary compounds are affected by numerous biotic and abiotic influences, including amount and wavelength of solar radiation. However, responses to shade and ultraviolet light restriction are inconsistent among plant species and compound class. We conducted a three-year study to evaluate the effect of shade and UV light restriction on total phenolic and terpene concentrations in tarbush. Sixty plants were randomly assigned to one of three treatments (control, UV light restriction, or 50% incident light restriction). Mean concentrations of total phenolics and total volatiles in tarbush were 82.4 and 12.5 mg/g DM, respectively. Total phenolics did not differ between UV-restricted and control plants, but were lower in shaded plants than the other treatments (P < 0.05). Total volatiles tended to be greater for the UV-restricted treatment than control plants (P = 0.056), with shaded plants not different from either treatment. Treatment effects were detected for 18 individual compounds (P < 0.05). Our results partially support the hypothesis that UV restriction and shading alter carbon-based secondary chemical concentrations. Published by Elsevier Ltd. C1 [Estell, Rick E.; Fredrickson, Ed L.; James, Darren K.] New Mexico State Univ, Jornada Expt Range, USDA ARS, Box 30003, Las Cruces, NM 88003 USA. [Fredrickson, Ed L.] Eastern Kentucky Univ, Dept Agr, 2 Carter Bldg, Richmond, KY 40475 USA. RP Estell, RE (reprint author), New Mexico State Univ, Jornada Expt Range, USDA ARS, Box 30003, Las Cruces, NM 88003 USA. EM restell@nmsu.edu NR 53 TC 0 Z9 0 U1 4 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0305-1978 EI 1873-2925 J9 BIOCHEM SYST ECOL JI Biochem. Syst. Ecol. PD APR PY 2016 VL 65 BP 108 EP 114 DI 10.1016/j.bse.2016.02.019 PG 7 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DK0JN UT WOS:000374599200015 ER PT J AU Mitchell, J Harben, R Sposito, G Shrestha, A Munk, D Miyao, G Southard, R Ferris, H Horwath, WR Kueneman, E Fisher, J Bottens, M Hogan, P Roy, R Komar, J Beck, D Reicosky, D Leinfelder-Miles, M Aegerter, B Six, J Barcellos, T Giacomazzi, D Sano, A Sanchez, J Crowell, M Diener, J Cordova, D Cordova, T Rossiter, J AF Mitchell, Jeffrey Harben, Ron Sposito, Garrison Shrestha, Anil Munk, Daniel Miyao, Gene Southard, Randy Ferris, Howard Horwath, William R. Kueneman, Eric Fisher, Judee Bottens, Monte Hogan, Phil Roy, Robert Komar, Jim Beck, Dwayne Reicosky, Don Leinfelder-Miles, Michelle Aegerter, Brenna Six, Johan Barcellos, Tom Giacomazzi, Dino Sano, Alan Sanchez, Jesse Crowell, Mike Diener, John Cordova, Darrell Cordova, Trevor Rossiter, Jerry TI Conservation agriculture: Systems thinking for sustainable farming SO CALIFORNIA AGRICULTURE LA English DT Editorial Material ID TILLAGE SYSTEMS; SOIL; EVAPORATION; VALLEY AB California is an unquestioned global leader in the productivity of its farms and the diversity and quality of its crops. However, there are significant threats to the sustainability of the state's agricultural systems. The scarcity of water has received great attention in recent years, but that is just one of many concerns. Fossil fuel use, carbon emissions, nitrate pollution of groundwater, labor cost and availability, air pollution and loss of soil fertility all present challenges to the long-term viability of farming in California. C1 [Mitchell, Jeffrey] Univ Calif Davis, Dept Plant Sci, Davis, CA USA. [Harben, Ron] USDA NRCS, Davis, CA USA. [Harben, Ron] Calif Assoc Resource Conservat Dist, Davis, CA USA. [Sposito, Garrison] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA USA. [Shrestha, Anil] Calif State Univ Fresno, Fresno, CA 93740 USA. [Southard, Randy; Horwath, William R.] Univ Calif Davis, Dept Land Air & Water Sci, Davis, CA USA. [Ferris, Howard] Univ Calif Davis, Dept Nematol, Davis, CA USA. [Kueneman, Eric; Fisher, Judee] FAO, I-00100 Rome, Italy. [Bottens, Monte] Calif Ag Solut, Madera, CA USA. [Beck, Dwayne] Dakota Lakes Res Farm, Pierre, SD USA. [Reicosky, Don] USDA ARS, Morris, MN USA. [Six, Johan] ETH, Dept Environm Syst Sci, Zurich, Switzerland. [Rossiter, Jerry] Cisco AG, Atwater, CA USA. RP Mitchell, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA USA. NR 9 TC 0 Z9 0 U1 22 U2 31 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 EI 2160-8091 J9 CALIF AGR JI Calif. Agric. PD APR-JUN PY 2016 VL 70 IS 2 BP 53 EP 56 PG 4 WC Agriculture, Multidisciplinary SC Agriculture GA DM7AT UT WOS:000376505100002 ER PT J AU Highland, S James, RR AF Highland, Steven James, R. R. TI The Similarity and Appropriate Usage of Three Honey Bee (Hymenoptera: Apidae) Datasets for Longitudinal Studies SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE honey bee; Apis mellifera; NASS-ABH data; Census of Agriculture data; state registration ID COLONY COLLAPSE DISORDER AB Honey bee (Apis mellifera L., Hymenoptera: Apidae) colonies have experienced profound fluctuations, especially declines, in the past few decades. Long-term datasets on honey bees are needed to identify the most important environmental and cultural factors associated with these changes. While a few such datasets exist, scientists have been hesitant to use some of these due to perceived shortcomings in the data. We compared data and trends for three datasets. Two come from the US Department of Agriculture's National Agricultural Statistics Service (NASS), Agricultural Statistics Board: one is the annual survey of honey-producing colonies from the Annual Bee and Honey program (ABH), and the other is colony counts from the Census of Agriculture conducted every five years. The third dataset we developed from the number of colonies registered annually by some states. We compared the long-term patterns of change in colony numbers among the datasets on a state-by-state basis. The three datasets often showed similar hive numbers and trends varied by state, with differences between datasets being greatest for those states receiving a large number of migratory colonies. Dataset comparisons provide a method to estimate the number of colonies in a state used for pollination versus honey production. Some states also had separate data for local and migratory colonies, allowing one to determine whether the migratory colonies were typically used for pollination or honey production. The Census of Agriculture should provide the most accurate long-term data on colony numbers, but only every five years. C1 [Highland, Steven] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. [James, R. R.] USDA ARS, Crop Prod & Protect, George Washington Carver Ctr, 5601 Sunnyside Ave, Beltsville, MD 20705 USA. RP Highland, S (reprint author), Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. EM highland.geo@gmail.com; Rosalind.James@ars.usda.gov FU USDA Agricultural Research Service, Pollinating Insects-Biology, Management, and Systematics Laboratory, Logan, UT FX We would like to thank the state apiarists who kindly provided us with registration data from their states, including S. Chard, B. Bloetscher, S. Brunner, T. Jadczak, J. Marra, S. Parise, R. Reiners, M. Studer, and. D. Westervelt. We would like to thank B. Boess from the USDA-NASS for providing clarifying discussions about their surveys, and comments on earlier drafts of the manuscript. We would also thank W. Meikle for his review comments. This project was funded by the USDA Agricultural Research Service, Pollinating Insects-Biology, Management, and Systematics Laboratory, Logan, UT. NR 9 TC 0 Z9 0 U1 8 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 277 EP 282 DI 10.1093/ee/nvv227 PG 6 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000001 PM 26884549 ER PT J AU Cornelius, ML Buffington, ML Talamas, EJ Gates, MW AF Cornelius, Mary L. Buffington, Matthew L. Talamas, Elijah J. Gates, Michael W. TI Impact of the Egg Parasitoid, Gryon pennsylvanicum (Hymenoptera: Scelionidae), on Sentinel and Wild Egg Masses of the Squash Bug (Hemiptera: Coreidae) in Maryland SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE biological control; egg parasitism; egg predation; pest management ID YELLOW VINE DISEASE; LEPTOGLOSSUS-OCCIDENTALIS HEIDEMANN; ANASA-TRISTIS HEMIPTERA; MARMORATED STINK BUG; TRISSOLCUS-BASALIS; ASHMEAD HYMENOPTERA; BIOLOGICAL-CONTROL; NATURAL ENEMIES; NEZARA-VIRIDULA; POPULATION-DYNAMICS AB Seasonal changes in egg parasitism and predation rates on sentinel (laboratory-reared) and wild (naturally occurring) egg masses of the squash bug, Anasa tristis (De Geer), were evaluated in squash fields in Maryland from June through September in 2013 and 2014. Rates of egg parasitism were significantly higher on wild egg masses than on sentinel egg masses. Squash bug nymphal emergence was significantly higher on sentinel egg masses than on wild egg masses. Between the first week of July and the first week of September of both survey years, squash bug nymphs emerged from 24.2% of wild eggs compared with 46.2% of sentinel eggs and parasitoids emerged from 55.7% of wild eggs compared with only 21.8% of sentinel eggs. Sentinel egg masses significantly underestimated the rate of natural egg parasitism. The egg parasitoid, Gryon pennsylvanicum (Ashmead), was responsible for over 99% of parasitism of squash bug eggs. There was a significant negative correlation between parasitoid emergence and nymphal emergence, suggesting that parasitoids were able to suppress squash bug populations. The average rate of parasitoid emergence peaked on wild egg masses on the fifth week of July at 72.8%, whereas the average rate of nymphal emergence from wild egg masses was < 20% from the fifth week of July until the first week of September. These results demonstrate that G. pennsylvanicum was able to efficiently track wild squash bug eggs throughout the season and that it has the potential to be an effective biological control agent of the squash bug in Maryland. C1 [Cornelius, Mary L.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave,Bldg 007, Beltsville, MD 20705 USA. [Buffington, Matthew L.; Talamas, Elijah J.; Gates, Michael W.] USDA ARS, Systemat Entomol Lab, Natl Museum Nat Hist, Smithsonian Inst, 10th & Constitut Ave NW,MRC 168, Washington, DC 20560 USA. RP Cornelius, ML (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave,Bldg 007, Beltsville, MD 20705 USA. EM mary.cornelius@ars.usda.gov; matt.buffington@ARS.USDA.GOV; elijah.talamas@ars.usda.gov; michael.gates@ars.usda.gov NR 45 TC 1 Z9 1 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 367 EP 375 DI 10.1093/ee/nvv228 PG 9 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000011 PM 26759367 ER PT J AU Chen, F Shi, J Keena, M AF Chen, Fang Shi, Juan Keena, Melody TI Evaluation of the Effects of Light Intensity and Time Interval After the Start of Scotophase on the Female Flight Propensity of Asian Gypsy Moth (Lepidoptera: Erebidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Asian gypsy moth; female flight; light intensity; scotophase ID LYMANTRIA-DISPAR LEPIDOPTERA; DAILY ONSET; TEMPERATURE; CAPABILITY; INSECTS; AMBIENT; RUSSIA AB Asian gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae), females are capable of flight, but little is known about what causes the variation in flight propensity that has been observed. The female flight propensity and capability of Asian gypsy moth from seven geographic populations (three from China, two from Russia, one from Japan, and one from Korea) were compared under all combinations of three light intensities (0.05, 0.10, and 0.40 lux) and during three time intervals after the start of scotophase. A total of 567 females were flight tested. Female flight propensity, time to initiate walking, fanning, and flying, and duration of fanning differed significantly among geographic populations. Females were less likely to voluntarily fly during the 0-1-h time interval after the start of scotophase than during the later time intervals (1-2 and 2-3 h), suggesting that the light intensity cue has to occur at the correct time after the expected start of scotophase for flight initiation. Light intensity did not significantly affect the proportion of females that voluntarily flew, but did impact the timing of the walking and fanning preflight behaviors. The interaction between light intensity and time interval after the start of scotophase had a significant effect on the proportion of females that fanned. The proportion of females with sustained flight capability varied among the populations evaluated. These results may aid in determining the risk of Asian gypsy moth dispersal, but further work is needed to assess other factors that play a role in flight propensity. C1 [Chen, Fang; Shi, Juan] Beijing Forestry Univ, Coll Forestry, Beijing Key Lab Forest Pest Control, 35 Qinghua East Rd, Beijing 100083, Peoples R China. [Keena, Melody] US Forest Serv, No Res Stn, USDA, 51 Mill Pond Rd, Hamden, CT 06514 USA. RP Keena, M (reprint author), US Forest Serv, No Res Stn, USDA, 51 Mill Pond Rd, Hamden, CT 06514 USA. EM chen-fang866@163.com; shi_juan@263.net; mkeena@fs.fed.us FU Fundamental Research for the Central Universities [BLYJ201306]; National Natural Science Foundation of China [31170613]; China Scholarship Council (CSC); Forest Service Northern Research Station FX We thank G. Bradford, S. Limbu, A. Martin, P. Moore, J. Richards, and V. Sanchez for assistance with rearing and the flight tests at the Forest Service quarantine facility in Ansonia, CT. We thank S. Limbu, V. Sanchez, and the two anonymous reviewers for offering suggestions that improved this manuscript. This work was funded by the Fundamental Research for the Central Universities (BLYJ201306), the National Natural Science Foundation of China (31170613), the China Scholarship Council (CSC), and the Forest Service Northern Research Station. NR 26 TC 0 Z9 0 U1 2 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 404 EP 409 DI 10.1093/ee/nvv222 PG 6 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000016 PM 26748672 ER PT J AU Cordeiro, EMG Campbell, JF Phillips, TW AF Cordeiro, Erick M. G. Campbell, James F. Phillips, Thomas W. TI Movement and Orientation Decision Modeling of Rhyzopertha dominica (Coleoptera: Bostrichidae) in the Grain Mass SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE lesser grain borer; model selection; AIC; wheat; stored product ID CRYPTOLESTES-FERRUGINEUS COLEOPTERA; STORED WHEAT; TEMPERATURE-GRADIENTS; FLIGHT INITIATION; INSECT DENSITY; F COLEOPTERA; PROBE TRAP; LAEMOPHLOEIDAE; SELECTION; BORER AB Grain stored in bins is initially a relatively homogenous resource patch for stored-product insects, but over time, spatial pattern in insect distribution can form, due in part to insect movement patterns. However, the factors that influence stored-product insect movement patterns in grain are not well-understood. This research focused on the movement of the lesser grain borer, Rhyzopertha dominica (F.), within a simulated wheat grain mass (vertical monolayer of wheat) and the identification of factors that contribute to overall and upward movement (age since adult emergence from an infested kernel [1, 7, and 14 d], sex, strain, and different levels of environment quality). We also used the model selection approach to select the most relevant factors and determine the relationships among them. Three-week-old adults tended to stay closer to the surface compared with 1- or 2-wk-old insects. Also, females tended to be more active and to explore a larger area compared with males. Explored area and daily displacement were also significantly strain-dependent, and increasing grain infestation level decreased daily displacement and explored area. Variation in movement pattern is likely to influence the formation of spatial pattern and affect probability to disperse. Understanding movement behavior within a grain bin is crucial to designing better strategies to implement and interpret monitoring programs and to target control tactics. C1 [Cordeiro, Erick M. G.; Phillips, Thomas W.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Campbell, James F.] ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Campbell, JF (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM cordeiro.emg@gmail.com; james.campbell@ars.usda.gov; twp1@ksu.edu RI Cordeiro, Erick/P-7042-2015 FU National Council of Scientific and Technological Development (CNPq) FX We thank R. Hammel and A. Redmon for excellent technical support. Appreciation is also expressed to the National Council of Scientific and Technological Development (CNPq) for financial support provided. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture or Kansas State University. USDA and Kansas State University are equal opportunity employers. This paper is contribution number 16-065-J of the Kansas Agricultural Experiment Station. NR 40 TC 0 Z9 0 U1 1 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 410 EP 419 DI 10.1093/ee/nvv232 PG 10 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000017 PM 26837621 ER PT J AU Cira, TM Venette, RC Aigner, J Kuhar, T Mullins, DE Gabbert, SE Hutchison, WD AF Cira, Theresa M. Venette, Robert C. Aigner, John Kuhar, Thomas Mullins, Donald E. Gabbert, Sandra E. Hutchison, W. D. TI Cold Tolerance of Halyomorpha halys (Hemiptera: Pentatomidae) Across Geographic and Temporal Scales SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE cold hardiness; chill intolerance; supercooling point; invasive species ID HARDINESS; INSECTS; TEMPERATURE; HETEROPTERA AB The brown marmorated stink bug, Halyomorpha halys (StAyenl), is native to eastern Asia and is presently invading North America. Little is known about the exposure to and effects of winter temperatures in newly invaded regions on H. halys. The overwintering habitats that this species utilizes vary greatly in their thermal buffering capacity. They naturally overwinter in aggregations beneath loose bark on trees and in cliff outcroppings, but will also commonly aggregate in buildings. Effects of cold temperatures such as mortality and freezing have yet to be quantified in the invading population. We report that H. halys is chill intolerant (i.e., dies before reaching its freezing point), and that the degree of cold tolerance of populations in North America differs by season, sex, and acclimation location. The mean winter supercooling point (+/- SEM) of individuals acclimated in Minnesota was -17.06A degrees C A +/- 0.13 and in Virginia was -13.90A degrees C A +/- 0.09. By using laboratory assays of lower lethal temperatures and ambient air temperature records, we accurately forecasted mortality for field experiments in Minnesota and Virginia. Temperature refugia provided by human-built structures are likely crucial for overwintering survival during atypically cold winters and possibly contribute to the northern geographic range expansion of this economically damaging insect in the temperate climates of North America. C1 [Cira, Theresa M.; Hutchison, W. D.] Univ Minnesota, Dept Entomol, 219 Hodson Hall 1980 Folwell Ave, St Paul, MN 55108 USA. [Venette, Robert C.] US Forest Serv, No Res Stn, USDA, 1992 Folwell Ave, St Paul, MN 55108 USA. [Aigner, John; Kuhar, Thomas; Mullins, Donald E.; Gabbert, Sandra E.] Virginia Tech, Dept Entomol, 170 Drillfield Dr, Blacksburg, VA 24061 USA. RP Cira, TM (reprint author), Univ Minnesota, Dept Entomol, 219 Hodson Hall 1980 Folwell Ave, St Paul, MN 55108 USA. EM cirax002@umn.edu; rvenette@fs.fed.us; daigner@vt.edu; tkuhar@vt.edu; mullinsd@vt.edu; sgabbert@vt.edu; hutch002@umn.edu FU Minnesota's Discovery, Research, and InnoVation Economy (MnDRIVE) Global Food Ventures graduate fellowship at the University of Minnesota; United States Department of Agriculture National Institute of Food and Agriculture Specialty Crop Research Initiative (USDA-NIFA-SCRI) Grant [2011-51181-30937]; Virginia Department of Agriculture and Consumer Services United States Department of Agriculture Specialty Crop Block Grant FX This project would not have been possible without the dedication of several people, especially Eric Burkness, Lindsey Christianson, Amy Morey, Robert Koch, Katherine Kamminga, Tracy Leskey, and Doo-Hyung Lee, as well as Jaana Iverson, Benjamin Aigner, and Ashley Lohr. We thank Nathaniel Cira for reviewing this paper prior to submission. We also appreciate the use of the laboratory facilities of the United States Department of Agriculture Forest Service Northern Research Station. This project was funded in part by a Minnesota's Discovery, Research, and InnoVation Economy (MnDRIVE) Global Food Ventures graduate fellowship at the University of Minnesota, United States Department of Agriculture National Institute of Food and Agriculture Specialty Crop Research Initiative (USDA-NIFA-SCRI) Grant 2011-51181-30937, and the Virginia Department of Agriculture and Consumer Services United States Department of Agriculture Specialty Crop Block Grant for the 2011 fiscal year. NR 32 TC 3 Z9 3 U1 6 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 484 EP 491 DI 10.1093/ee/nvv220 PG 8 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000025 PM 26744454 ER PT J AU Branson, DH AF Branson, David H. TI Drought Impacts on Competition in Phoetaliotes nebrascensis (Orthoptera Acrididae) in a Northern Mixed Grassland SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE grasshopper; drought; competition; rangeland; reproduction ID GRASSHOPPER POPULATIONS; INSECT HERBIVORES; FOOD LIMITATION; RESPONSES; WEATHER; ECOSYSTEMS; ABUNDANCE; DYNAMICS; NITROGEN; DENSITY AB Global climate change is predicted to significantly modify patterns of precipitation, making it critical to develop a better understanding of how this will modify biotic interactions. Short-term to decadal-scale weather patterns can impact grasshopper population dynamics, but drought impacts on grasshoppers have rarely been studied in manipulative experiments. A cage experiment was conducted in eastern Montana to examine the impact of intra- and interspecific competition and precipitation manipulation treatments on performance of a common melanopline grasshopper Phoetaliotes nebrascensis (Thomas). High-density and drought treatments had similarly strong negative impacts on food availability. Proportional grasshopper survival did not differ significantly by treatment, but density dependence was evident in both body size and reproductive traits. The impact of precipitation and density treatments on grasshopper body size and reproduction were typically similar in magnitude and much larger than interspecific competition, with the exception of male femur length. Even with high late summer precipitation, drought had strong effects on individual body size and future reproduction. This study provides valuable information on population dynamics of an abundant grasshopper, with moderate precipitation reductions negatively affecting reproduction and body size. No positive impacts of drought as predicted by the plant stress hypothesis were observed. The study reinforces the need to examine drought manipulations to better predict grasshopper population changes due to changing climate conditions. C1 [Branson, David H.] ARS, USDA, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. RP Branson, DH (reprint author), ARS, USDA, Northern Plains Agr Res Lab, 1500 N Cent Ave, Sidney, MT 59270 USA. EM dave.branson@ars.usda.gov NR 40 TC 0 Z9 0 U1 3 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 492 EP 499 DI 10.1093/ee/nvv225 PG 8 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000026 PM 26744453 ER PT J AU Lu, ZB Dang, C Han, NS Shen, ZC Peng, YF Stanley, D Ye, GY AF Lu, Zengbin Dang, Cong Han, Naishun Shen, Zhicheng Peng, Yufa Stanley, David Ye, Gongyin TI The New Transgenic cry1Ab/vip3H Rice Poses No Unexpected Ecological Risks to Arthropod Communities in Rice Agroecosystems SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Transgenic rice; crylAb; vip3H; epsps; arthropod ID BACILLUS-THURINGIENSIS BERLINER; VEGETATIVE INSECTICIDAL PROTEIN; HELIOTHIS-VIRESCENS LEPIDOPTERA; CANDIDA COLLEMBOLA ISOTOMIDAE; RESISTANT GM RICE; BT RICE; FIELD CONDITIONS; NONTARGET ARTHROPOD; DELTA-ENDOTOXIN; CNAPHALOCROCIS-MEDINALIS AB The ecological risks to nontarget organisms should be rigorously assessed before Bt crops are released. Here, the impacts of a new Cry1Ab/Vip3H rice line on arthropod communities in rice agroecosystems were evaluated across 3 yr. Arthropods collected via vacuum were sorted into five guilds. The abundance and proportion of each guild as well as community-level parameters were determined in Cry1Ab/Vip3H and control rice fields. Changes in arthropod species assemblage over sampling dates were investigated by principal response curves (PRCs). Cry1Ab/Vip3H rice did not exert significant impacts on the seasonal density and proportion of each guild, except parasitoids. Detritivore seasonal density, but not its relative abundance, was significantly affected by Cry1Ab/Vip3H rice. Four community indices (species richness S, Shannon-Wiener index H', Simpson index D, and evenness index J') were similar between rice types. PRCs revealed a slight community difference between rice types in the past two tested years, with rice types accounting for 1.0-3.5% of the variance among arthropod communities. However, sampling dates explain 32.1-67.6% for these community differences. Of the 46 taxa with higher species weights, 26.1% of the taxa were significantly different, including seven taxa with higher abundance and five with lower density in Cry1Ab/Vip3H rice fields. These differences may be attributed to change in abundance of prey or hosts but not to direct effects of Bt proteins. We infer that this new Cry1Ab/Vip3H rice line poses no unintended ecological risks to the arthropod community. C1 [Lu, Zengbin] Shandong Acad Agr Sci, Inst Plant Protect, Jinan 250100, Peoples R China. [Lu, Zengbin; Dang, Cong; Han, Naishun; Shen, Zhicheng; Ye, Gongyin] Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China. [Lu, Zengbin; Dang, Cong; Han, Naishun; Shen, Zhicheng; Ye, Gongyin] Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Agr Entomol, Hangzhou 310058, Zhejiang, Peoples R China. [Peng, Yufa] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. RP Ye, GY (reprint author), Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China.; Ye, GY (reprint author), Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Agr Entomol, Hangzhou 310058, Zhejiang, Peoples R China. EM luzengbin12345@163.com; dangcong2013@163.com; hannaishunbbg@163.com; zcshen@zju.edu.cn; yfpeng@vip.qq.com; stanleyd@missouri.edu; chu@zju.edu.cn FU Special Research Projects for Developing Transgenic Plants [2014ZX08011-001]; China National Science Fund for Innovative Research Group of Biological Control [31321063] FX We thank Prof. Q. Y. Tang (Zhejiang University, Zhejiang, China) for helping with our data analyses and Dr M. Chen (Monsanto Company, 700 Chesterfield Parkway W., GG3I Chesterfield, MO 63017, USA) for critically polishing our manuscript. Financial support was provided by the Special Research Projects for Developing Transgenic Plants (2014ZX08011-001) and China National Science Fund for Innovative Research Group of Biological Control (Grant no. 31321063). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 82 TC 0 Z9 0 U1 6 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 518 EP 525 DI 10.1093/ee/nvv215 PG 8 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000029 PM 26721297 ER PT J AU Meihls, LN Frank, DL Ellersieck, MR Hibbard, BE AF Meihls, Lisa N. Frank, Daniel L. Ellersieck, Mark R. Hibbard, Bruce E. TI Development and Characterization of MIR604 Resistance in a Western Corn Rootworm Population (Coleoptera: Chrysomelidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Bt resistance; heritability ID DIABROTICA-VIRGIFERA-VIRGIFERA; MULTIPLE TRANSATLANTIC INTRODUCTIONS; ERROR COMPONENT MODEL; INSECT RESISTANCE; BACILLUS-THURINGIENSIS; CARBON-DIOXIDE; TRANSGENIC MAIZE; FITNESS COSTS; HOST LOCATION; BT CROPS AB mCry3A is one of only four proteins licensed for commercial use in Diabrotica control. Utilizing a colony of western corn rootworm, Diabrotica virgifera virgifera LeConte, selected for resistance to mCry3A, we evaluated how mCry3A resistance was inherited and whether fitness costs were associated with mCry3A resistance. Reciprocal crosses between a selected colony and a control colony were performed; resulting progeny along with parent colonies were evaluated in dose toxicity assays, greenhouse assays, and seedling assays. Dose toxicity assay results were inconclusive, as the highest dose of protein tested did not produce sufficient mortality for accurate LC50 calculation. In whole-plant greenhouse assays on mCry3A-expressing corn, larval relative survival of the selected female x control male reciprocal cross was similar to that of the selected colony, while that of the control female x selected male was intermediate between the mCry3-selected colony and the control colony. However, when adult relative survival in whole-plant greenhouse assays was examined, no significant difference between the reciprocal crosses and the two parent colonies was detected. Heritability calculations based on both larval (0.66) and adult (1.03) survival data indicate that resistance to mCry3A is not inherited in a recessive manner. The selected colony was removed from selection pressure and evaluated after three or eight generations of removal. At three generations of removal from selection, a slight decrease in larval relative survival was detected compared with the selected colony. At eight generations of removal from selection, larval relative survival was comparable with that of the selected colony. C1 [Meihls, Lisa N.; Hibbard, Bruce E.] USDA ARS, Plant Genet Res Unit, 205 Curtis Hall, Columbia, MO 65211 USA. [Frank, Daniel L.] W Virginia Univ, Extens Serv, Agr & Nat Resources Unit, Morgantown, WV 26506 USA. [Ellersieck, Mark R.] Univ Missouri, Agr Expt Stn Statistician, 307E Middlebush, Columbia, MO 65211 USA. RP Meihls, LN (reprint author), USDA ARS, Plant Genet Res Unit, 205 Curtis Hall, Columbia, MO 65211 USA. EM lisa.meihls@ars.usda.gov; dlfrank@mail.wvu.edu; ellersieckm@missouri.edu; bruce.hibbard@ars.us-da.gov FU Syngenta; Biotechnology Risk Assessment Grant Program competitive grant from USDA National Institute of Food and Agriculture [2009-33120-20256]; Monsanto Company's Corn Rootworm Knowledge Research Program FX We thank Julie Barry for technical assistance with insect rearing and Custom Bio-Products for performing dose-toxicity assays. We thank Syngenta for providing seed and protein necessary for experiments. Research for this study was funded by Syngenta and by the Biotechnology Risk Assessment Grant Program competitive grant 2009-33120-20256 from the USDA National Institute of Food and Agriculture. A grant from Monsanto Company's Corn Rootworm Knowledge Research Program helped fund the analysis and writing of this manuscript. NR 64 TC 0 Z9 0 U1 2 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2016 VL 45 IS 2 BP 526 EP 536 DI 10.1093/ee/nvv226 PG 11 WC Entomology SC Entomology GA DM5JO UT WOS:000376385000030 PM 26834186 ER PT J AU Takeda, F Glenn, DM AF Takeda, F. Glenn, D. M. TI Susceptibility of blackberry flowers to freezing temperatures SO EUROPEAN JOURNAL OF HORTICULTURAL SCIENCE LA English DT Article DE exotherm; freezing chamber; ice barrier; infrared thermography; radiation frost; Rubus; thermocouple ID EASTERN UNITED-STATES; THORNLESS BLACKBERRY; ICE FORMATION; COLD INJURY; PLANTS; THERMOGRAPHY; TOLERANCE; CULTIVARS; BLUEBERRY; BUDS AB In this study, freezing tolerance of 'Triple Crown' blackberry flowers at different stages of reproductive development (tight bud to green drupe) was determined. One method involved placing whole plants in a radiation frost chamber and taking plants to -1.0 degrees C, -2.0 degrees C, -3.0 degrees C, and -4.0 degrees C for 1 h (total of 8 plants for each selected temperature). After freeze treatments, all buds, open flowers, and green fruit were evaluated for damage. Exposure to -1.0 degrees C or -2 degrees C temperatures for 1 h did not injure the corolla, gynoecium or androecium. However, damage was observed in gynoecium as indicated by tissue discoloration in the ovaries, styles and stigmas when blackberry were exposed to 3.0 degrees C for 1 h. An exposure to -4.0 degrees C for 1 h resulted in the injury of all floral parts. In the second method, thermocouple wires were inserted into tight buds, open flowers, and green fruit of potted blackberry plants and the chamber temperature was slowly lowered to -4.0 degrees C on 8 separate runs. The thermocouple readings were captured at 5 s intervals to characterize the exotherms in tight buds and open flowers. In all runs, tight buds and open flowers generated exotherms between -2.5 degrees C and -2.8 degrees C. These findings suggested that flowers at tight-bud stage and flowers already at anthesis froze at similar temperatures. In another study, an infrared (IR) thermal imaging camera was used to determine ice formation in blackberry plants by capturing thermographic images of plant surfaces at 1 ms intervals. IR thermography revealed that ice nucleation was initiated in the cane and ice propagated into flower shoots and then quickly into several flowers and flower buds. The results of these studies suggested that the buds at tight stage have similar susceptibility to freezing temperatures to those that have already opened. The results of this study suggest that we need to reconsider the relationship between developmental stages of flower bud, actual tissue and ambient temperatures, and susceptibility to spring frost damage in blackberries. C1 [Takeda, F.; Glenn, D. M.] ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Takeda, F (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM fumi.takeda@ars.usda.gov NR 23 TC 1 Z9 1 U1 5 U2 6 PU INT SOC HORTICULTURAL SCIENCE-ISHS PI LEUVEN PA PO BOX 500, LEUVEN, 3001, BELGIUM SN 1611-4426 EI 1611-4434 J9 EUR J HORTIC SCI JI Eur. J. Hortic. Sci. PD APR PY 2016 VL 81 IS 2 BP 115 EP 121 DI 10.17660/eJHS.2016/81.2.5 PG 7 WC Horticulture SC Agriculture GA DM3AQ UT WOS:000376219600005 ER PT J AU Yee, WL Alston, DG AF Yee, Wee L. Alston, Diane G. TI Sucrose Mixed with Spinosad Enhances Kill and Reduces Oviposition of Rhagoletis indifferens (Diptera: Tephritidae) Under Low Food Availability SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE western cherry fruit fly; spinosyn insecticide; yeast extract plus sucrose; sweet cherries; oviposition ID FRUIT-FLIES DIPTERA; FEEDING RESPONSES; PROTEIN BAITS; FLY DIPTERA; CERATITIS-CAPITATA; MELON FLIES; MORTALITY; SPRAYS; ATTRACTION; GF-120 AB Whether sugar mixed with insecticides enhances kill of western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), may depend on insecticide rate and food availability. Here, we tested the hypothesis that sucrose mixed with the insecticide spinosad (in the Entrust (R) SC formulation) enhances kill of adults and reduces oviposition when food is scarce. Three- to 5-d-old flies were exposed to a low or high rate of dried spinosad or spinosad mixed with sucrose in the presence of (a) supplemental food in the form of yeast extract + sucrose (YE + S) and sweet cherries (Prunus avium [L.] L.) or (b) only sweet cherries. Cherries were a food source and an oviposition substrate. At the low spinosad rate, sucrose enhanced fly kill over spinosad alone under both food conditions the first 4 d or during all 7 d of experiments. At the high spinosad rate, sucrose enhanced kill only when supplemental food was absent. Sucrose-enhanced spinosad did not reduce oviposition versus spinosad alone at either spinosad rate when supplemental food was present but it did at both spinosad rates when only cherries were present. Results suggest that sucrose mixed with the formulation of spinosad tested here in low-volume sprays could be useful for managing R. indifferens in low-food environments, but it offers no benefit in preventing oviposition when applied in food-rich environments. C1 [Yee, Wee L.; Alston, Diane G.] ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Alston, Diane G.] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. RP Yee, WL (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov NR 36 TC 0 Z9 0 U1 4 U2 6 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2016 VL 51 IS 2 BP 101 EP 112 PG 12 WC Entomology SC Entomology GA DM5VJ UT WOS:000376418000001 ER PT J AU Layman, ML Lundgren, JG AF Layman, Marissa L. Lundgren, Jonathan G. TI Increasing Trophic Complexity Influences Aphid Attendance by Ants (Hymenoptera: Formicidae) and Predation SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Coccinellidae; Coleomegilla maculata; food web; Formica montana; honeydew; Myzus persicae; Pieris rapae ID IMPORTED FIRE ANT; PIERIS-RAPAE; ARTHROPOD PREDATION; LABORATORY COLONIES; SOLENOPSIS-INVICTA; DENSITY-DEPENDENCE; FOOD DISTRIBUTION; SUGAR COMPOSITION; TENDING ANTS; LADY BEETLE AB Species that are involved in multitrophic interactions are affected by the trophic levels that are above and below them in both indirect and direct ways. In this experiment, interactions among ants (Formica montana Wheeler; Hymenoptera: Formicidae), aphids (Myzus persicae [Sulzer]; Hemiptera: Aphididae), lepidopteran eggs (Pieris rapae [L.]; Lepidoptera: Pieridae), and lady beetles (Coleomegilla maculata DeGeer; Coleoptera: Coccinellidae) were examined on canola (Brassica napus L.; Brassicales: Brassicaceae) to determine whether aphids mediate interactions between ants and eggs and if aphid-tending ants will protect these species from predation by the lady beetles. The first trial examined how ant attendance affected aphid and egg survival over 72 h when these herbivores were present on their own or in combination. The second trial examined these same interactions when a lady beetle was included in the system. Ants consumed significantly more aphids when this prey was offered alone than when eggs were offered alongside. When lady beetles were present, ants did not consume aphids. Ants and lady beetles consumed nearly all of the eggs regardless of whether aphids were present or not. When a lady beetle was absent, ant attendance increased significantly, but only when eggs and aphids were present in the system together. Ants visited the aphids less frequently when a lady beetle was present in the system, possibly due to increased competition. This trend was consistent over time in all treatments. All of this suggests that ants can be an important source of pest management of other herbivores (and even of the aphids that they are attending) but that these interactions are mediated by whether an additional predator is present within the system. C1 [Layman, Marissa L.; Lundgren, Jonathan G.] ARS, USDA, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. [Layman, Marissa L.] S Dakota State Univ, Biol Microbiol Dept, Brookings, SD 57007 USA. RP Lundgren, JG (reprint author), ARS, USDA, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM jgl.entomology@gmail.com NR 63 TC 0 Z9 0 U1 3 U2 5 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2016 VL 51 IS 2 BP 151 EP 164 PG 14 WC Entomology SC Entomology GA DM5VJ UT WOS:000376418000006 ER PT J AU Giovannoni, J AF Giovannoni, James TI Harnessing epigenome modifications for better crops SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Editorial Material DE Auxin; DNA methylation; epigenome; genetic diversity; paramutation; sulfurea; tomato ID ARABIDOPSIS-THALIANA; EVOLUTION AB Chemical DNA modifications such as methylation influence translation of the DNA code to specific genetic outcomes. While such modifications can be heritable, others are transient, and their overall contribution to plant genetic diversity remains intriguing but uncertain. In this issue, Gouil and colleagues (pages 2655-2664) characterize the epigenome phenomenon termed 'paramutation' underlying a tomato photosynthesis-related gene defect, and in doing so expand our understanding of how epigenome modifications are conferred with exciting implications for crop improvement. C1 [Giovannoni, James] USDA, Robert W Holley Ctr, Tower Rd,Cornell Univ Campus, Ithaca, NY 14853 USA. [Giovannoni, James] USDA, Boyce Thompson Inst, Tower Rd,Cornell Univ Campus, Ithaca, NY 14853 USA. RP Giovannoni, J (reprint author), USDA, Robert W Holley Ctr, Tower Rd,Cornell Univ Campus, Ithaca, NY 14853 USA.; Giovannoni, J (reprint author), USDA, Boyce Thompson Inst, Tower Rd,Cornell Univ Campus, Ithaca, NY 14853 USA. EM james.giovannoni@ars.usda.gov NR 13 TC 1 Z9 1 U1 6 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD APR PY 2016 VL 67 IS 9 BP 2535 EP 2537 DI 10.1093/jxb/erw143 PG 3 WC Plant Sciences SC Plant Sciences GA DM5JV UT WOS:000376385800002 PM 27162274 ER PT J AU Zhang, JX Yuan, H Yang, Y Fish, T Lyi, SM Thannhauser, TW Zhang, LG Li, L AF Zhang, Junxiang Yuan, Hui Yang, Yong Fish, Tara Lyi, Sangbom M. Thannhauser, Theodore W. Zhang, Lugang Li, Li TI Plastid ribosomal protein S5 is involved in photosynthesis, plant development, and cold stress tolerance in Arabidopsis SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Arabidopsis; cold stress tolerance; photosynthesis; plastid ribosomal protein; proteomics; RPS5 ID MOLECULAR CHARACTERIZATION; PROTEOMIC ANALYSIS; PHYTOENE SYNTHASE; POTATO-TUBERS; KNOCK-OUT; CHLOROPLAST; GENE; RNA; TRANSLATION; IDENTIFICATION AB Plastid ribosomal proteins are essential components of protein synthesis machinery and have diverse roles in plant growth and development. Mutations in plastid ribosomal proteins lead to a range of developmental phenotypes in plants. However, how they regulate these processes is not fully understood, and the functions of some individual plastid ribosomal proteins remain unknown. To identify genes responsible for chloroplast development, we isolated and characterized a mutant that exhibited pale yellow inner leaves with a reduced growth rate in Arabidopsis. The mutant (rps5) contained a missense mutation of plastid ribosomal protein S5 (RPS5), which caused a dramatically reduced abundance of chloroplast 16S rRNA and seriously impaired 16S rRNA processing to affect ribosome function and plastid translation. Comparative proteomic analysis revealed that the rps5 mutation suppressed the expression of a large number of core components involved in photosystems I and II as well as many plastid ribosomal proteins. Unexpectedly, a number of proteins associated with cold stress responses were greatly decreased in rps5, and overexpression of the plastid RPS5 improved plant cold stress tolerance. Our results indicate that RPS5 is an important constituent of the plastid 30S subunit and affects proteins involved in photosynthesis and cold stress responses to mediate plant growth and development. C1 [Zhang, Junxiang; Zhang, Lugang] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Area, Coll Hort, Yangling 712100, Peoples R China. [Zhang, Junxiang; Yuan, Hui; Li, Li] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA. [Yang, Yong; Fish, Tara; Lyi, Sangbom M.; Thannhauser, Theodore W.; Li, Li] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. Shenyang Agr Univ, Coll Hort, Shenyang 110866, Peoples R China. RP Li, L (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY 14853 USA.; Li, L (reprint author), Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM ll37@cornell.edu RI Yuan, Hui/H-6723-2016 FU China Scholarship Council (CSC) FX We thank Dr. Xiangjun Zhou for his generous help and technical advice, and Mamta Srivastava for help with the subcellular localization analysis. JZ acknowledges the support of the China Scholarship Council (CSC). NR 52 TC 0 Z9 0 U1 11 U2 22 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD APR PY 2016 VL 67 IS 9 BP 2731 EP 2744 DI 10.1093/jxb/erw106 PG 14 WC Plant Sciences SC Plant Sciences GA DM5JV UT WOS:000376385800019 PM 27006483 ER PT J AU Yoo, J Lee, J Kwon, SI Chung, KH Lee, DH Choi, IM Mattheis, JP Kang, IK AF Yoo, Jingi Lee, Jinwook Kwon, Soon-Il Chung, Kyeong Ho Lee, Dong Hoon Choi, In Myung Mattheis, James P. Kang, In-Kyu TI Differences in Ethylene and Fruit Quality Attributes during Storage in New Apple Cultivars SO KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY LA English DT Article DE flesh firmness; internal ethylene concentration; Malus x domestica Borkh.; soluble solids concentration; titratable acidity ID CONTROLLED-ATMOSPHERE STORAGE; 1-METHYLCYCLOPROPENE 1-MCP; POSTHARVEST TREATMENT; MALUS-DOMESTICA; TERM STORAGE; SHELF-LIFE; HARVEST; MATURITY; BIOSYNTHESIS; SUNRISE AB Physiological characteristics of five new apple cultivars from the Korean apple breeding program were evaluated as a function of harvest time and storage after harvest. Internal ethylene concentration (IEC), flesh firmness, soluble solids concentration (SSC), and titratable acidity (TA) were measured in 'Summer Dream', 'Summer King', 'Green Ball', 'Picnic', and 'Hwangok' apples at harvest, during shelf life at 20 degrees C, and one day after cold storage at 0.5 degrees C in air. IEC increased during shelf life in 'Summer Dream', 'Summer King', and 'Green Ball' but not in 'Picnic' or 'Hwangok', regardless of harvest time. Flesh firmness decreased towards harvest time and decreased gradually with time in cold storage only in the former three cultivars. In turn, IEC increased during cold storage in the first three cultivars but not for the last two cultivars, irrespective of harvest time. Changes in SSC and TA did not consistently relate to harvest time or storage period but TA tended to decrease as IEC increased. Furthermore, IEC was negatively correlated with flesh firmness except in the 'Green Ball' cultivar but the significance level was much greater in 'Summer Dream' and 'Summer King' (p < 0.0001) than in 'Picnic' (p < 0.01) or 'Hwangok' (p < 0.05) cultivars. Flesh firmness was positively correlated with TA in the first three cultivars but not in the last two cultivars. Overall, the results indicate that cultivars for which IEC increased after harvest had reduced flesh firmness and TA after storage. C1 [Yoo, Jingi; Kang, In-Kyu] Kyungpook Natl Univ, Dept Hort Sci, Daegu 41566, South Korea. [Lee, Jinwook] Mokpo Natl Univ, Dept Hort Sci, Muan 58554, South Korea. [Kwon, Soon-Il; Chung, Kyeong Ho] RDA, Natl Inst Hort & Herbal Sci, Apple Res Inst, Kunwi 39000, South Korea. [Lee, Dong Hoon] RDA, Natl Inst Hort & Herbal Sci, Planning & Coordinat Div, Wonju 55365, South Korea. [Choi, In Myung] RDA, Natl Inst Hort & Herbal Sci, Fruit Res Div, Wonju 55365, South Korea. [Mattheis, James P.] USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. RP Kang, IK (reprint author), Kyungpook Natl Univ, Dept Hort Sci, Daegu 41566, South Korea. EM kangik@knu.ac.kr FU Rural Development Administration, Republic of Korea [PJ01164002] FX Financial support for this research was supported by a grant from 2014 Research Fund (PJ01164002) of Rural Development Administration, Republic of Korea. NR 33 TC 1 Z9 1 U1 2 U2 3 PU KOREAN SOC HORTICULTURAL SCIENCE PI JEOLLABUK-DO PA C/O NATL INST HORTICULTURAL & HERBAL SCIENCE, 100, NONGSAENGMYEONG-RO, ISEO-MYEON, WANJU-GUN, JEOLLABUK-DO, 565-852, SOUTH KOREA SN 1226-8763 J9 KOREAN J HORTIC SCI JI Korean J. Hortic. Sci. Technol. PD APR PY 2016 VL 34 IS 2 BP 257 EP 268 DI 10.12972/kjhst.20160027 PG 12 WC Horticulture SC Agriculture GA DM7PQ UT WOS:000376552900007 ER PT J AU Hawkins, TS Schiff, N Wilson, AD Leininger, TD Devall, MS AF Hawkins, Tracy S. Schiff, Nathan Wilson, A. Dan Leininger, Theodor D. Devall, Margaret S. TI Growth and competitive abilities of the federally endangered Lindera melissifolia and the potentially invasive Brunnichia ovata in varying densities, hydrologic regimes, and light availabilities SO BOTANY LA English DT Article DE plant competition; endangered species; invasive species; pondberry; redvine ID PLANT COMPETITION; MISSISSIPPI; PLASTICITY; LAURACEAE; DESIGNS; FOREST; HERBS AB Brunnichia ovata (Walter) Shinners is a native, perennial, woody vine with the potential to become an aggressive competitor of the federally endangered shrub Lindera melissifolia (Walt.) Blume. Our study simulated habitat disturbances to hydrologic regime and light availability that may occur naturally, or through active management practices aimed at ensuring the sustainability of L. melissifolia, and we determined the species responses to these changes. First-year plants of L. melissifolia and B. ovata were grown at varying densities, in flooding or nonflooding treatments, and receiving 100%, 47%, or 21% light availabilities. For both species, density effects, in combination with light availability and flooding regime influenced total biomass accumulation. Brunnichia ovata exhibited a high degree of plasticity with respect to biomass allocated between above-and below-ground tissues in response to flooding, whereas biomass allocation in L. melissifolia was relatively unaffected. Interspecific competition occurred primarily in nonflooding treatments. Our study highlighted the complexity of the relationship of L. melissifolia and B. ovata with regard to functional trait responses to changes in abiotic and biotic factors, and indicated that it will be necessary to consider entire plant community responses to mitigate increased competitive interactions and ensure the survival of L. melissifolia populations. C1 [Hawkins, Tracy S.] US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Mississippi State, MS 39762 USA. [Schiff, Nathan; Wilson, A. Dan; Leininger, Theodor D.; Devall, Margaret S.] US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. RP Hawkins, TS (reprint author), US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Mississippi State, MS 39762 USA. EM tracyhawkins@fs.fed.us NR 40 TC 0 Z9 0 U1 3 U2 4 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 1916-2790 EI 1916-2804 J9 BOTANY JI Botany PD APR PY 2016 VL 94 IS 4 BP 269 EP 276 DI 10.1139/cjb-2015-0171 PG 8 WC Plant Sciences SC Plant Sciences GA DM0HK UT WOS:000376024100004 ER PT J AU Smith, KT Arbellay, E Falk, DA Sutherland, EK AF Smith, Kevin T. Arbellay, Estelle Falk, Donald A. Sutherland, Elaine Kennedy TI Macroanatomy and compartmentalization of recent fire scars in three North American conifers SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE fire injury; fire history; dendrochronology; wound pathology; reaction zone; barrier zone; bluestain; conifer defense ID TREE MORTALITY; DISCOLORED WOOD; PONDEROSA PINE; LIVING TREES; FORESTS; REGIMES; MOUNTAINS; HEARTWOOD; SEVERITY; INSIGHTS AB Fire scars are initiated by cambial necrosis caused by localized lethal heating of the tree stem. Scars develop as part of the linked survival processes of compartmentalization and wound closure. The position of scars within dated tree ring series is the basis for dendrochronological reconstruction of fire history. Macroanatomical features were described for western larch (Larix occidentalis Nutt.), ponderosa pine (Pinus ponderosa Douglas ex P. Lawson & C. Lawson), and Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) injured by fire in 2003 and harvested in 2011 at the Lolo National Forest near Missoula, Montana, USA. Bark scorch did not necessarily indicate the formation of a scar. Wound-initiated discoloration inward from the scar face was bounded tangentially by reaction zones. In western larch, the transition between earlywood and latewood was much less abrupt in woundwood rings than in rings formed the same year but not associated with a scar. Wood formed the year after injury contained tangential rows of resin ducts in the earlywood. Compartmentalization plays a key role in resisting the spread of infection and the loss of healthy sapwood and heartwood. Wound closure restores some degree of circumferential continuity of the vascular cambium and reinforces stem structure. The terminology presented here should facilitate communication among tree pathologists, wound anatomists, and dendrochronologists. C1 [Smith, Kevin T.] USDA, US Forest Serv, No Res Stn, Durham, NH USA. [Arbellay, Estelle] Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC V5Z 1M9, Canada. [Falk, Donald A.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Falk, Donald A.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. [Sutherland, Elaine Kennedy] USDA, US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA. RP Smith, KT (reprint author), USDA, US Forest Serv, No Res Stn, Durham, NH USA. EM ktsmith@fs.fed.us NR 46 TC 1 Z9 1 U1 8 U2 14 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD APR PY 2016 VL 46 IS 4 BP 535 EP 542 DI 10.1139/cjfr-2015-0377 PG 8 WC Forestry SC Forestry GA DL9EY UT WOS:000375945800010 ER PT J AU Meyer, MD Bulaon, B MacKenzie, M Safford, HD AF Meyer, Marc D. Bulaon, Beverly MacKenzie, Martin Safford, Hugh D. TI Mortality, structure, and regeneration in whitebark pine stands impacted by mountain pine beetle in the southern Sierra Nevada SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE whitebark pine; mountain pine beetle; Sierra Nevada; resistance; resilience ID WESTERN UNITED-STATES; BLISTER RUST; GREATER YELLOWSTONE; CLIMATE-CHANGE; BARK BEETLES; FORESTS; ELEVATION; CALIFORNIA; OUTBREAKS; PATTERNS AB Whitebark pine (Pinus albicaulis Engelm.) is vulnerable to mountain pine beetle (Dendroctonus ponderosae Hopkins) attack throughout western North America, but beetle outbreaks in the southwestern portion of the range (i.e., Sierra Nevada) have been spatially limited until recently. We examined patterns of mortality, structure, and regeneration in whitebark pine stands impacted by mountain pine beetle in the southern Sierra Nevada. Mortality was greatest in medium to large diameter (>10-20 cm dbh) trees, resulting in declines in mean and maximum tree diameter and tree size class diversity following an outbreak. Severity of beetle attack was positively related to mean tree diameter and density. Density of young (<3 years old) whitebark pine seedling clusters was positively related to severity of beetle attack on mature stands. All sites showed a stable production of whitebark pine regeneration within at least the past 30-40 years, with a pulse of new seedlings in the past 3 years in beetle-impacted stands. Our results show that mountain pine beetle outbreaks in the southern Sierra Nevada result in substantial changes in whitebark pine stand structure and suggest low resistance but high resilience to initial attack, especially in the absence of white pine blister rust. C1 [Meyer, Marc D.] USDA Forest Serv Pacific Southwest Reg, 1600 Tollhouse Rd, Clovis, CA 93611 USA. [Bulaon, Beverly; MacKenzie, Martin] USDA, Forest Serv, South Sierra Shared Serv Area, Forest Hlth Protect, 19777 Greenley Rd, Sonora, CA 95370 USA. [Safford, Hugh D.] USDA, Forest Serv, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. [Safford, Hugh D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. RP Meyer, MD (reprint author), USDA Forest Serv Pacific Southwest Reg, 1600 Tollhouse Rd, Clovis, CA 93611 USA. EM mdmeyer@fs.fed.us FU USDA Forest Service Pacific Southwest Region; USDA Forest Service Forest Health Protection Whitebark Pine Restoration Program FX Funding for this project was provided by the USDA Forest Service Pacific Southwest Region and USDA Forest Service Forest Health Protection Whitebark Pine Restoration Program. We sincerely thank Lisa Zander, Stephanie Eyes, Victor Biasotti, Amy Brown, Edward Stephan, Raymond Gutierrez, Katy Wilkinson, and Shana Gross for their invaluable assistance in collecting field data. We also thank Scott Kusumoto, Michele Slaton, and Kathleen Nelson of the Inyo National Forest for logistical and technical support. We acknowledge the Sierra Nevada Research Station Yosemite Field Station for logistic support. NR 42 TC 0 Z9 0 U1 13 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD APR PY 2016 VL 46 IS 4 BP 572 EP 581 DI 10.1139/cjfr-2015-0464 PG 10 WC Forestry SC Forestry GA DL9EY UT WOS:000375945800015 ER PT J AU Lin, X Faridi, N Casola, C AF Lin, Xuan Faridi, Nurul Casola, Claudio TI An Ancient Transkingdom Horizontal Transfer of Penelope-Like Retroelements from Arthropods to Conifers SO GENOME BIOLOGY AND EVOLUTION LA English DT Article DE lateral transmission; transposable elements; Dryads; loblolly pine ID MULTIPLE SEQUENCE ALIGNMENT; NON-LTR RETROTRANSPOSONS; TRANSPOSABLE ELEMENTS; DROSOPHILA-VIRILIS; EVOLUTIONARY GENOMICS; EUKARYOTIC EVOLUTION; EXTANT GYMNOSPERMS; PLANT GENOMES; GENE-TRANSFER; RUST FUNGI AB Comparative genomics analyses empowered by the wealth of sequenced genomes have revealed numerous instances of horizontal DNA transfers between distantly related species. In eukaryotes, repetitive DNA sequences known as transposable elements (TEs) are especially prone to move across species boundaries. Such horizontal transposon transfers, or HTTs, are relatively common within major eukaryotic kingdoms, including animals, plants, and fungi, while rarely occurring across these kingdoms. Here, we describe the first case of HTT from animals to plants, involving TEs known as Penelope-like elements, or PLEs, a group of retrotransposons closely related to eukaryotic telomerases. Using a combination of in situ hybridization on chromosomes, polymerase chain reaction experiments, and computational analyses we show that the predominant PLE lineage, EN(+)PLEs, is highly diversified in loblolly pine and other conifers, but appears to be absent in other gymnosperms. Phylogenetic analyses of both protein and DNA sequences reveal that conifers EN(+)PLEs, or Dryads, form a monophyletic group clustering within a clade of primarily arthropod elements. Additionally, no EN(+)PLEs were detected in 1,928 genome assemblies from 1,029 nonmetazoan and nonconifer genomes from 14 major eukaryotic lineages. These findings indicate that Dryads emerged following an ancient horizontal transfer of EN(+)PLEs from arthropods to a common ancestor of conifers approximately 340 Ma. This represents one of the oldest known interspecific transmissions of TEs, and the most conspicuous case of DNA transfer between animals and plants. C1 [Lin, Xuan; Faridi, Nurul; Casola, Claudio] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [Faridi, Nurul] USDA Forest Serv, Southern Inst Forest Genet, Southern Res Stn, Saucier, MS USA. RP Casola, C (reprint author), Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. EM ccasola@tamu.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [TEX0-1-9599]; Texas A&M AgriLife Research; Texas A&M Forest Service FX The authors are grateful to the following institutions and individuals for providing specimens for DNA samples: The Lovett Pinetum Charitable Foundation, the Missouri Botanical Garden, St. Louis MO, the Texas A&M University S.M. Tracy Herbarium, Michael Arnold, James Carcano, Sofia Casola, Amanda Hulse-Kemp, Jeremy Johnson, Matthew Kent, Dale Kruse, Jesse Meik, Michelle Lawing, Carol Loopstra, Jacob Owen, David Stelly, and James Solomon. They thank Jeffrey Puryear, Mengmeng Lu, and Fei Wang for technical assistance. They are especially grateful to Michelle Lawing, Cedric Feschotte, Ellen Pritham, Tomasz Koralewski, and two anonymous reviewers for comments on the manuscript. This material is based upon work that was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number TEX0-1-9599, the Texas A&M AgriLife Research, and the Texas A&M Forest Service. NR 73 TC 2 Z9 2 U1 5 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1759-6653 J9 GENOME BIOL EVOL JI Genome Biol. Evol. PD APR PY 2016 VL 8 IS 4 BP 1252 EP 1266 DI 10.1093/gbe/evw076 PG 15 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DM1SL UT WOS:000376126400023 PM 27190138 ER PT J AU Sanders, GJ Juvancic-Heltzel, J Williamson, ML Roemmich, JN Feda, DM Barkley, JE AF Sanders, Gabriel J. Juvancic-Heltzel, Judith Williamson, Megan L. Roemmich, James N. Feda, Denise M. Barkley, Jacob E. TI The Effect of Increasing Autonomy Through Choice on Young Children's Physical Activity Behavior SO JOURNAL OF PHYSICAL ACTIVITY & HEALTH LA English DT Article DE variety; play; accelerometer; health ID ENERGY-DENSITY; PEER INFLUENCE; NORMAL-WEIGHT; VARIETY; OVERWEIGHT; EXERCISE; INTERVENTION; DETERMINANTS; CHILDHOOD; ENJOYMENT AB Background: Increasing autonomy by manipulating the choice of available physical activity options in a laboratory setting can increase physical activity in older children and adults. However, the effect of manipulating the number of physically active choices has yet to be examined in young children in a gymnasium environment. Methods: Twenty children (n = 10 girls, 6.1 +/- 1.4 years old) individually participated in 2 [low choice (LC), high choice (HC)] free-choice activity conditions for 30 minutes in a 4360 square foot gymnasium. Children had access to 2 or 8 physical activity options in the LC and HC conditions, respectively. Physical activity behavior was measured via accelerometry. Results: Children's 30-minute accelerometer counts increased (P < .03) from the LC (2675 +/- 294 counts.min(-1)) to the HC (3224 +/- 280 counts.min(-1)) condition. Conclusions: Providing greater autonomy through choice of a greater number of physically active options increased young children's physical activity participation by 20.5%. C1 [Sanders, Gabriel J.] No Kentucky Univ, Dept Kinesiol & Hlth, Highland Hts, KY 41076 USA. [Juvancic-Heltzel, Judith] Univ Akron, Dept Exercise Sci, Akron, OH 44325 USA. [Williamson, Megan L.] Kent State Univ, Dept Lifespan Dev & Educ Sci, Kent, OH 44242 USA. [Roemmich, James N.] ARS, USDA, PA, Grand Forks, ND USA. [Feda, Denise M.] Univ Buffalo, Dept Exercise Sci, Buffalo, NY USA. [Barkley, Jacob E.] Kent State Univ, Sch Exercise Leisure & Sport, Kent, OH 44242 USA. RP Sanders, GJ (reprint author), No Kentucky Univ, Dept Kinesiol & Hlth, Highland Hts, KY 41076 USA. EM sandersgl@nku.edu NR 41 TC 1 Z9 1 U1 2 U2 2 PU HUMAN KINETICS PUBL INC PI CHAMPAIGN PA 1607 N MARKET ST, PO BOX 5076, CHAMPAIGN, IL 61820-2200 USA SN 1543-3080 EI 1543-5474 J9 J PHYS ACT HEALTH JI J. Phys. Act. Health PD APR PY 2016 VL 13 IS 4 BP 428 EP 432 DI 10.1123/jpah.2015-0171 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DM3BG UT WOS:000376221200011 PM 26444033 ER PT J AU Walter, CA Raiff, DT Burnham, MB Gilliam, FS Adams, MB Peterjohn, WT AF Walter, Christopher A. Raiff, Devon T. Burnham, Mark B. Gilliam, Frank S. Adams, Mary Beth Peterjohn, William T. TI Nitrogen fertilization interacts with light to increase Rubus spp. cover in a temperate forest SO PLANT ECOLOGY LA English DT Article DE Nitrogen deposition; Herbaceous layer; Fertilization; Forest understory; Rubus; Bramble ID SPECIES-DIVERSITY; HERBACEOUS VEGETATION; FLOOR VEGETATION; HARVARD FOREST; DEPOSITION; PLANT; RESPONSES; AVAILABILITY; COMPETITION; COMMUNITY AB Nitrogen additions have caused species composition changes in many ecosystems by facilitating the growth of nitrophilic species. After 24 years of nitrogen fertilization in a 40 year-old stand at the Fernow Experimental Forest (FEF) in Central Appalachia, USA, the cover of Rubus spp. has increased from 1 to 19 % of total herbaceous-layer cover. While Rubus spp. are generally associated with high-light conditions that are created after a disturbance event, some species are also known to be nitrophilic. We investigated whether the increase in cover in Rubus spp. was due to either nitrogen, light, or an interaction between these two factors. To test for the effect of nitrogen and light on Rubus spp. cover, we compared the relative cover of Rubus spp. among fertilized and unfertilized watersheds and among fertilized and unfertilized experimental plots, using estimates of canopy openness as a covariate. Rubus spp. plants were also grown ex situ in a field experiment using a 2-way factorial design, measuring leaf area, and using two levels of nitrogen and three levels of light. The effect of nitrogen fertilization on relative Rubus spp. cover depended on canopy openness in the watersheds (F = 17.57, p = 0.0002) and experimental plots (F = 25.04, p = 0.0047). A similar effect for leaf area was also observed among plants grown in the field experiment (F = 4.12, p = 0.0247). Our results confirm that, although Rubus spp. at FEF are nitrophilic, they require sufficient light to increase their cover. Furthermore, the dominance of Rubus spp. in the herbaceous layer likely contributes to the observed decline in species diversity. C1 [Walter, Christopher A.; Raiff, Devon T.; Burnham, Mark B.; Peterjohn, William T.] W Virginia Univ, Dept Biol, 53 Campus Dr, Morgantown, WV 26505 USA. [Gilliam, Frank S.] Marshall Univ, Dept Biol Sci, One John Marshall Dr,Sci Bldg 350, Huntington, WV 25755 USA. [Adams, Mary Beth] US Forest Serv, USDA, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. RP Walter, CA (reprint author), W Virginia Univ, Dept Biol, 53 Campus Dr, Morgantown, WV 26505 USA. EM cwalte12@mix.wvu.edu FU National Science Foundation [DEB-0417678, DEB-1019522, DBI-0849917]; West Virginia University Eberly College of Arts and Sciences; David Blaydes Scholarship FX We thank Rachel Arrick, Bobby Clemer, Jessica Graham, Joe Hilgenberg, Lily Hill, Justin Lego, and Hoff Lindberg for helping with field and farm excursions. We are grateful to Jen Chandler, Jessi Brie Turner, and Jim McGraw for their insightful comments and suggestions in writing this manuscript. Funding for this research was provided by the National Science Foundation from their Long-Term Research in Environmental Biology program (Grant Nos. DEB-0417678 and DEB-1019522) and their Research Experience for Undergraduates program (Grant No. DBI-0849917), as well as the West Virginia University Eberly College of Arts and Sciences and the David Blaydes Scholarship. Finally, special thanks to the staff of the Fernow Experimental Forest, past and present, for the foresight to begin and maintain long-term ecological research in central Appalachia. NR 65 TC 0 Z9 0 U1 7 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD APR PY 2016 VL 217 IS 4 BP 421 EP 430 DI 10.1007/s11258-016-0583-z PG 10 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DM3UF UT WOS:000376271500006 ER PT J AU Tepe, EJ Anderson, GJ Spooner, DM Bohs, L AF Tepe, Eric J. Anderson, Gregory J. Spooner, David M. Bohs, Lynn TI Relationships among wild relatives of the tomato, potato, and pepino SO TAXON LA English DT Article DE Americas; phylogeny; potato; Solanaceae; Solanum; taxonomy ID SOLANUM SECTION BASARTHRUM; CONSERVED ORTHOLOG SEQUENCES; PHYLOGENETIC-RELATIONSHIPS; HERPYSTICHUM SOLANACEAE; CULTIVATED POTATOES; EVOLUTION; PETOTA; SYSTEMATICS; INFERENCE; MURICATUM AB With ca. 200 species, the informally named Potato clade represents one of the larger subgroups of the estimated 1500 species of Solanum. Because its members include the potato (S. tuberosum), tomato (S. lycopersicum), and pepino (S. muricatum), it is the most economically important elude in the genus. These crop species and their close relatives have been the focus of intensive research, but relationships among major lineages of the Potato Glade remain poorly understood. In this study, we use sequences from the nuclear ITS and waxy (GBSSI), and plastid trnT-trnF and trnS-trnG to estimate a phylogeny and further explore relationships within the Potato Glade. With increased sampling over past studies, the Potato Glade emerges as a strongly supported elude and comprises 12-13 subclades which, for the most part, correspond to traditionally recognized sections. Solanum sect. Regmandra is sister to the rest of the lineages of the Potato Glade which are, in turn, organized into two major subclades: (1) sections Anarrhichomenum, Articulatum, Basarthrum, Etuberosum, Juglandifolia, Lycopersicoides, Lycopersicon, and Petota, and (2) sections Herpystichum and Pteroidea. As in all other studies including these groups, sections Etuberosum, Juglandifolia, Lycopersicoides, Lycopersicon, and Petota form a strongly supported Glade. Solanum oxycoccoides, a high-elevation species endemic to north-central Peru, was tentatively assigned to several groups within Solanum based on morphological evidence, but instead the species represents an independent lineage within the Potato Glade, sister to the first major subclade. A key to the sections of the Potato clade is provided. C1 [Tepe, Eric J.] Univ Cincinnati, Dept Biol Sci, Cincinnati, OH 45221 USA. [Anderson, Gregory J.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. [Spooner, David M.] Univ Wisconsin, Dept Hort, USDA ARS, Madison, WI 53706 USA. [Bohs, Lynn] Univ Utah, Dept Biol, Salt Lake City, UT 84112 USA. RP Tepe, EJ (reprint author), Univ Cincinnati, Dept Biol Sci, Cincinnati, OH 45221 USA. EM eric.tepe@uc.edu FU NSF [DEB-0316614, DEB-1020509]; W.S. Turrell Herbarium Fund, Miami University (MU) [214] FX We thank S. Stern, F. Farruggia, S. Leiva, M.P. Moreno, M. Vargas, and F. Villao for assistance in the field, and F. Farruggia, K. Leo, S. Stern, and A. Van Wagenen for laboratory assistance; the curator at B for permission to extract DNA from S. oxycoccoides-2; and two anonymous reviewers whose suggestions improved this manuscript. This work was supported in part by an allocation of computing time from the Ohio Supercomputer Center (http://www.osc.edu) to EJT, the NSF through the "PBI: Solanum-a worldwide treatment" (DEB-0316614) and the "BSI: Caterpillars and Parasitoids of the Eastern Andes" grants (DEB-1020509), and by the W.S. Turrell Herbarium Fund, Miami University (MU), No. 214. NR 75 TC 0 Z9 0 U1 15 U2 20 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD APR PY 2016 VL 65 IS 2 BP 262 EP 276 PG 15 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DL7LZ UT WOS:000375823500004 ER PT J AU Allen, WC Castlebury, LA Rossman, AY AF Allen, W. Cavan Castlebury, Lisa A. Rossman, Amy Y. TI (2425) Proposal to conserve the name Discula (Diaporthales: Gnomoniaceae) with a conserved type SO TAXON LA English DT Editorial Material C1 [Allen, W. Cavan] N Carolina State Univ, Dept Plant Pathol, Box 7616, Raleigh, NC 27695 USA. [Allen, W. Cavan; Castlebury, Lisa A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Rossman, Amy Y.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. RP Rossman, AY (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. EM amydianer@yahoo.com NR 11 TC 1 Z9 1 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD APR PY 2016 VL 65 IS 2 BP 388 EP 388 PG 1 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DL7LZ UT WOS:000375823500019 ER PT J AU Sanders, M McPartlin, D Moran, K Guo, YR Eeckhout, M O'Kennedy, R De Saeger, S Maragos, C AF Sanders, Melanie McPartlin, Daniel Moran, Kara Guo, Yirong Eeckhout, Mia O'Kennedy, Richard De Saeger, Sarah Maragos, Chris TI Comparison of Enzyme-Linked Immunosorbent Assay, Surface Plasmon Resonance and Biolayer Interferometry for Screening of Deoxynivalenol in Wheat and Wheat Dust SO TOXINS LA English DT Article DE deoxynivalenol; monoclonal antibodies; immunosensor; wheat; wheat dust ID GRAIN DUST; MONOCLONAL-ANTIBODIES; MYCOTOXIN ANALYSIS; IMMUNOASSAY; AFFINITY; FUNGI AB A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 g/kg, LOD wheat dust: 458 g/kg). C1 [Sanders, Melanie; Maragos, Chris] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. [McPartlin, Daniel; Moran, Kara; O'Kennedy, Richard] Dublin City Univ, Natl Ctr Sensor Res, Sch Biotechnol, Dublin 9, Ireland. [McPartlin, Daniel; Moran, Kara; O'Kennedy, Richard] Dublin City Univ, Biomed Diagnost Inst, Dublin 9, Ireland. [Guo, Yirong] Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310029, Zhejiang, Peoples R China. [Eeckhout, Mia] Univ Ghent, Fac Biosci Engn, Dept Appl Biosci, Valentin Vaerwyckweg 1, B-9000 Ghent, Belgium. [De Saeger, Sarah] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, Dept Bioanal, Ottergemsesteenweg 460, B-9000 Ghent, Belgium. RP Sanders, M (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM melanie.sanders@ars.usda.gov; daniel.mcpartlin2@mail.dcu.ie; kara.moran24@mail.dcu.ie; yirongguo@zju.edu.cn; mia.eeckhout@ugent.be; richard.okennedy@dcu.ie; sarah.desaeger@ugent.be; chris.maragos@ars.usda.gov FU Ghent University Special Research Foundation [BOF01SM2113]; Science Foundation Ireland [14/1A/2646]; Oak Ridge Associated Universities (ORAU); MycoHunt project [243633] FX The authors would like to thank Ghent University Special Research Foundation (BOF01SM2113), Science Foundation Ireland (14/1A/2646) and Oak Ridge Associated Universities (ORAU) for the financial support. The 10H10 monoclonal DON antibody was developed in the frame of the MycoHunt project (FP7 grant agreement number 243633). NR 30 TC 0 Z9 0 U1 10 U2 19 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD APR PY 2016 VL 8 IS 4 AR 103 DI 10.3390/toxins8040103 PG 14 WC Toxicology SC Toxicology GA DL8AL UT WOS:000375861700018 PM 27077883 ER PT J AU Ashworth, DJ Yates, SR AF Ashworth, Daniel J. Yates, Scott R. TI Breakthrough of 1,3-Dichloropropene and Chloropicrin from 600-mg XAD-4 Air Sampling Tubes SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Fumigation; Air quality; Sorbent tubes; Flow rate; Sampling time AB Accurately measuring air concentrations of agricultural fumigants is important for the regulation of air quality. Understanding the conditions under which sorbent tubes can effectively retain such fumigants during sampling is critical in mitigating chemical breakthrough from the tubes and facilitating accurate concentration measurements. Using laboratory experiments, we studied the effects of air flow rate (1001000 mL min(-1)) and sampling time (2-16 h) on the breakthrough of co-applied chloropicrin (CP) and 1,3dichloropropene (1,3-D) from 600-mg XAD-4 sorbent tubes. Due to the reversible adsorption of the chemicals, it was not possible to determine a tube adsorption capacity that was true across all flow and sample time conditions. Flow rate exerted the stronger influence on breakthrough, particularly for CP, with flow rates in excess of 200 mL min(-1) resulting in significant system losses even at the shortest sampling time (2 h). A flow rate of 200 mL min(-1) should therefore not be exceeded, irrespective of flow rate. With the use of a single tube (no backup), sampling times up to 4 h showed no system losses (100 % retention). Using a primary and backup tube, sampling periods up to 16 h also resulted in retention of all the added chemical masses. The information will be useful in establishing effective air quality monitoring programs following fumigation events. C1 [Ashworth, Daniel J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Yates, Scott R.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. RP Ashworth, DJ (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. EM daniel.ashworth@ars.usda.gov NR 9 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD APR PY 2016 VL 227 IS 4 AR 127 DI 10.1007/s11270-016-2814-2 PG 6 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA DM3WJ UT WOS:000376277100016 ER PT J AU Motamed, M McPhail, L Williams, R AF Motamed, Mesbah McPhail, Lihong Williams, Ryan TI Corn Area Response to Local Ethanol Markets in the United States: A Grid Cell Level Analysis SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE corn acreage; ethanol refineries; biofuels; grid cell data; agricultural land use; Q15; Q16 ID IMPACTS; PLANTS; LOCATION; ACREAGE; PRICES AB We measure corn and total agricultural area response to the biofuels boom in the United States from 2006 to 2010. Specifically, we use newly available micro-scale grid cell data to test whether a location's corn and total agricultural cultivation rose in response to the capacity of ethanol refineries in their vicinity. Based on these data, acreage in corn and overall agriculture not only grew in already-cultivated areas but also expanded into previously uncultivated areas. Acreage in corn and total agriculture also correlated with proximity to ethanol plants, though the relationship dampened over the time period. A formal estimation of the link between acreage and ethanol refineries, however, must account for the endogenous location decisions of ethanol plants and areas of corn supply. We present historical evidence to support the use of the US railroad network as a valid instrument for ethanol plant locations. Our estimates show that a location's neighborhood refining capacity exerts strong and significant effects on acreage planted in corn and total agricultural acreage. The largest impacts of ethanol plants were felt in locations where cultivation area was relatively low. This high-resolution evidence of ethanol impacts on local agricultural outcomes can inform researchers and policy-makers concerned with crop diversity, environmental sustainability, and rural economic development. C1 [Motamed, Mesbah; Williams, Ryan] USDA, Econ Res Serv, Washington, DC USA. [McPhail, Lihong] Commod Futures Trading Commiss, New York, NY USA. RP Motamed, M (reprint author), USDA, Econ Res Serv, Washington, DC USA. EM mmotamed@ers.usda.gov NR 37 TC 1 Z9 1 U1 5 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD APR PY 2016 VL 98 IS 3 BP 726 EP 743 DI 10.1093/ajae/aav095 PG 18 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DL8SE UT WOS:000375910900003 ER PT J AU Uyeda, KA Stow, DA O'Leary, JF Schmidt, IT Riggan, PJ AF Uyeda, Kellie A. Stow, Douglas A. O'Leary, John F. Schmidt, Ian T. Riggan, Philip J. TI Spatial variation of fuel loading within varying aged stands of chaparral SO APPLIED VEGETATION SCIENCE LA English DT Article DE Adenostoma fasciculatum; Biomass accumulation; California chaparral; Chamise; Post-fire recovery; Quercus berberidifolia; Scrub oak; Shrub; Stand age; Vegetation type map; Wildfire ID SOUTHERN-CALIFORNIA; CEANOTHUS-MEGACARPUS; FIRE MANAGEMENT; SUCCESSION; INTENSITY AB Questions: How do stand-level biomass and percentage of deadmaterial in chaparral vary as a function of stand age? How do the landscape properties of aggregation index and patch size vary in each of the dominant species groups as a function of stand age? Location: Stands of 7-, 28- and 68-yr-old chaparral, San Diego County, CA, US. Methods: We sampled and estimated above-ground biomass in 8 m x 8 m field plots. Each stand was classified into species groups using high-resolution imagery. After establishing coefficients for the average biomass per area for each species group and stand age using field data, we estimated biomass in 30 random plots in each stand age using the classified map. We also estimated biomass using pooled coefficients (average of the 28- and 68-yr-old biomass per area). We calculated landscape metrics in six 60 m 9 60 m study sites for each stand age. Results: While we found a statistically significant pattern of higher biomass in the older chaparral stands when using age-specific biomass coefficients, there was no statistical difference between the 28- and 68-yr-old stands using the more conservative pooled coefficients. Both approaches revealed a wide range in biomass in all age classes. We were not able to map dead biomass with sufficient accuracy to measure age-related differences, but no differences in the two older stands were obvious in the field. There were no significant differences in landscape metrics between the two older stands, and the differences observed in the younger stand might have been partially due to aspect differences. Conclusions: Our approach of using a combination of field plots and classification of remote sensing imagery is a valuable method for enlarging the effective study area in chaparral, and allowed us to better measure how widely biomass varies across the study area. High spatial resolution measurements of fuel properties could help support more detailed models of fire behaviour. C1 [Uyeda, Kellie A.; Stow, Douglas A.; O'Leary, John F.; Schmidt, Ian T.] San Diego State Univ, Dept Geog, 5500 Campanile Dr, San Diego, CA 92182 USA. [Uyeda, Kellie A.] Univ Calif Santa Barbara, Dept Geog, 1832 Ellison Hall, Santa Barbara, CA 93106 USA. [Riggan, Philip J.] US Forest Serv, Pacific Southwest Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. RP Uyeda, KA (reprint author), San Diego State Univ, Dept Geog, 5500 Campanile Dr, San Diego, CA 92182 USA.; Uyeda, KA (reprint author), Univ Calif Santa Barbara, Dept Geog, 1832 Ellison Hall, Santa Barbara, CA 93106 USA. EM kellie.uyeda@gmail.com; stow@mail.sdsu.edu; oleary@mail.sdsu.edu; itssit@gmail.com; priggan@fs.fed.us OI Uyeda, Kellie/0000-0001-7043-3870 FU USDA Forest Service [10-JV-11279701-10] FX We thank J. Truett, T. Hayes and J. Kraling from the USFS for arranging for site access. We also thank D. Rachels, C. Riggan, A. Brown, F. Uyeda, J. Jesu, B. Lo, B. Corcoran, D. Smith and Y. Granovskaya for assistance in the field. Funding for this project was provided by the USDA Forest Service through American Recovery and Reinvestment Act Agreement No. 10-JV-11279701-10: Airborne remote sensing to enable hazardous fuels reduction, forest health protection, rehabilitation and hazard mitigation activities on federal lands. NR 32 TC 3 Z9 3 U1 13 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD APR PY 2016 VL 19 IS 2 BP 267 EP 279 DI 10.1111/avsc.12209 PG 13 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DJ9PO UT WOS:000374545600011 ER PT J AU Hu, HF Knapp, BO Wang, GG Walker, JL AF Hu, Huifeng Knapp, Benjamin O. Wang, G. Geoff Walker, Joan L. TI Silvicultural treatments for converting loblolly pine to longleaf pine dominance: effects on ground layer and midstorey vegetation SO APPLIED VEGETATION SCIENCE LA English DT Article DE Ecological restoration; Fertilization; Functional group; Herbicide; Midstorey stem density ID PALUSTRIS MILL. SEEDLINGS; TAEDA L. STANDS; RESOURCE AVAILABILITY; CANOPY STRUCTURE; SITE PREPARATION; ECOSYSTEM; RESTORATION; OVERSTORY; MANAGEMENT; RESPONSES AB Questions: How do management practices used to enhance longleaf pine (Pinus palustris) seedling survival and growth under a loblolly pine (Pinus taeda) canopy alter the structure of midstorey and ground layer vegetation? Do management treatments achieve general restoration targets for longleaf pine ecosystem structure? Location: Marine Corps Base Camp Lejeune, NC, USA within the Middle Atlantic Coastal Plain Ecoregion. Methods: Four levels of timber harvest were applied to loblolly pine stands: Control (uncut, basal area similar to 16.2 m(2).ha(-1)), MedBA (residual basal area similar to 9.0 m(2).ha(-1)), LowBA (residual basal area similar to 6.4 m(2).ha(-1)) and Clearcut (residual basal area of 0 m(2).ha(-1)). Within each canopy treatment, we applied three cultural treatments selected to facilitate longleaf pine seedling success: NT (untreated), H (chemical control of woody vegetation) and H + F (chemical control plus fertilization). Vegetation responses, including the abundance (cover) of ground layer vegetation and midstorey stem densities, were reported for three growing seasons (2008-2010) following canopy removal. Results: The ground layer was dominated by woody vegetation, and total vegetation cover generally increased with increasing canopy removal. Canopy treatment effects varied through time. Clearcut plots had higher total herbaceous and graminoid cover than MedBA and Control plots in 2008, while woody cover was significantly lower on Control plots than on LowBA and Clearcut plots in 2009. Clearcut plots had higher densities of loblolly pines than Control plots in 2009 and 2010. The herbicide treatment reduced hardwood densities, but increased loblolly pine densities, especially in 2010. Conclusions: Successful restoration prescriptions are often site-specific because of different land-use history, climate, site characteristics and starting conditions. To achieve the restoration objective of creating an open midstorey with an herbaceous-dominated ground layer when converting loblolly pine stands to longleaf pine dominance on relatively productive sites with abundant hard woods and aggressive loblolly pine natural regeneration, canopy retention can slow the rate of development of loblolly pine regeneration and herbicides reduce hardwood stem densities. Frequent, repeated burning would likely be required to further reduce woody vegetation and increase the relative abundance of herbaceous vegetation. C1 [Hu, Huifeng; Knapp, Benjamin O.; Wang, G. Geoff] Clemson Univ, Dept Forestry & Environm Conservat, Clemson, SC 29634 USA. [Hu, Huifeng] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China. [Knapp, Benjamin O.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Walker, Joan L.] Clemson Univ, USDA, Forest Serv, Southern Res Stn, Clemson, SC 29634 USA. RP Wang, GG (reprint author), Clemson Univ, Dept Forestry & Environm Conservat, Clemson, SC 29634 USA. EM huifhu@ibcas.ac.cn; knappb@missouri.edu; gwang@clemson.edu; joanwalker@fs.fed.us FU Strategic Environmental Research and Development Program (SERDP) - Department of Defense (DoD) [RC-1474]; Department of Energy (DoE); Environmental Protection Agency (EPA) FX We are grateful to four anonymous reviews for their insightful comments and suggestions on an earlier version of this manuscript. Funding for this project (RC-1474) was provided by the Strategic Environmental Research and Development Program (SERDP) sponsored by the Department of Defense (DoD), Department of Energy (DoE) and Environmental Protection Agency (EPA). We would like to thank the following field technicians: Seth Cook, K. Hunter Leary, Joe Ledvina, Bryan Mudder, Erik Pearson, Shawna Reid, Lindsay Stewart, Carsyn Tennant and Evelyn Wenk for assistance with data collection. Dr. James R. Rieck, Department of Mathematical Sciences of Clemson University, provided statistical support. This paper is technical contribution number 6334 of the Clemson University Experiment Station. NR 50 TC 0 Z9 0 U1 7 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD APR PY 2016 VL 19 IS 2 BP 280 EP 290 DI 10.1111/avsc.12217 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DJ9PO UT WOS:000374545600012 ER PT J AU Rossel, RAV Behrens, T Ben-Dor, E Brown, DJ Dematte, JAM Shepherd, KD Shi, Z Stenberg, B Stevens, A Adamchuk, V Aichi, H Barthes, BG Bartholomeus, HM Bayer, AD Bernoux, M Bottcher, K Brodsky, L Du, CW Chappell, A Fouad, Y Genot, V Gomez, C Grunwald, S Gubler, A Guerrero, C Hedley, CB Knadel, M Morras, HJM Nocita, M Ramirez-Lopez, L Roudier, P Campos, EMR Sanborn, P Sellitto, VM Sudduth, KA Rawlins, BG Walter, C Winowiecki, LA Hong, SY Ji, W AF Rossel, R. A. Viscarra Behrens, T. Ben-Dor, E. Brown, D. J. Dematte, J. A. M. Shepherd, K. D. Shi, Z. Stenberg, B. Stevens, A. Adamchuk, V. Aichi, H. Barthes, B. G. Bartholomeus, H. M. Bayer, A. D. Bernoux, M. Bottcher, K. Brodsky, L. Du, C. W. Chappell, A. Fouad, Y. Genot, V. Gomez, C. Grunwald, S. Gubler, A. Guerrero, C. Hedley, C. B. Knadel, M. Morras, H. J. M. Nocita, M. Ramirez-Lopez, L. Roudier, P. Rufasto Campos, E. M. Sanborn, P. Sellitto, V. M. Sudduth, K. A. Rawlins, B. G. Walter, C. Winowiecki, L. A. Hong, S. Y. Ji, W. TI A global spectral library to characterize the world's soil SO EARTH-SCIENCE REVIEWS LA English DT Article DE Soil spectral library; Global soil dataset; Soil vis-NIR spectra; Vis-NIR spectroscopy; Multivariate statistics; Machine teaming; Wavelets ID INFRARED REFLECTANCE SPECTROSCOPY; LEAST-SQUARES REGRESSION; ORGANIC-CARBON CONTENT; IN-SITU CHARACTERIZATION; CLAY LOAM SOIL; NIR SPECTROSCOPY; PREDICTING SOIL; MIDINFRARED SPECTROSCOPY; QUANTITATIVE-ANALYSIS; MEDITERRANEAN SOILS AB Soil provides ecosystem services, supports human health and habitation, stores carbon and regulates emissions of greenhouse gases. Unprecedented pressures on soil from degradation and urbanization are threatening agro-ecological balances and food security. It is important that we learn more about soil to sustainably manage and preserve it for future generations. To this end, we developed and analyzed a global soil visible-near infrared (vis-NIR) spectral library. It is currently the largest and most diverse database of its kind. We show that the information encoded in the spectra can describe soil composition and be associated to land cover and its global geographic distribution, which acts as a surrogate for global climate variability. We also show the usefulness of the global spectra for predicting soil attributes such as soil organic and inorganic carbon, clay, silt, sand and iron contents, cation exchange capacity, and pH. Using wavelets to treat the spectra, which were recorded in different laboratories using different spectrometers and methods, helped to improve the spectroscopic modelling. We found that modelling a diverse set of spectra with a machine leaming algorithm can find the local relationships in the data to produce accurate predictions of soil properties. The spectroscopic models that we derived are parsimonious and robust, and using them we derived a harmonized global soil attribute dataset, which might serve to facilitate research on soil at the global scale. This spectroscopic approach should help to deal with the shortage of data on soil to better understand it and to meet the growing demand for information to assess and monitor soil at scales ranging from regional to global. New contributions to the library are encouraged so that this work and our collaboration might progress to develop a dynamic and easily updatable database with better global coverage. We hope that this work will reinvigorate our community's discussion towards larger, more coordinated collaborations. We also hope that use of the database will deepen our understanding of soil so that we might sustainably manage it and extend the research outcomes of the soil, earth and environmental sciences towards applications that we have not yet dreamed of. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Rossel, R. A. Viscarra; Chappell, A.; Ji, W.] CSIRO Land & Water, POB 1666, Canberra, ACT 2601, Australia. [Behrens, T.] Univ Tubingen, Inst Geog, Tubingen, Germany. [Ben-Dor, E.] Tel Aviv Univ, Remote Sensing & GIS Lab, Dept Geog, POB 39040, IL-69989 Tel Aviv, Israel. [Brown, D. J.] Washington State Univ, Pullman, WA 99164 USA. [Dematte, J. A. M.] Univ Sao Paulo, Coll Agr Luiz de Queiroz, Dept Soil Sci, Sao Paulo, Brazil. [Shepherd, K. D.; Winowiecki, L. A.] ICRAF, World Agroforestry Ctr, POB 30677-00100, Nairobi, Kenya. [Shi, Z.; Ji, W.] Zhejiang Univ, Coll Environm & Resource Sci, Inst Appl Remote Sensing & Informat Technol, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China. [Stenberg, B.] Swedish Univ Agr Sci, Dept Soil & Environm, POB 234, S-53223 Skara, Sweden. [Stevens, A.] UC Louvain, Earth & Life Inst, Georges Lemaitre Ctr Earth & Climate Res, Louvain, Belgium. [Adamchuk, V.; Ji, W.] McGill Univ, Dept Bioresource Engn, Ste Anne De Bellevue, PQ, Canada. [Aichi, H.] Higher Sch Agr, Mograne, Tunisia. [Barthes, B. G.; Bernoux, M.] IRD, UMR Eco & Sols, SupAgro, 2 Pl Viala, F-34060 Montpellier, France. [Bartholomeus, H. M.] Wageningen Univ, Lab Geoinformat Sci & Remote Sensing, POB 47, NL-6700 AA Wageningen, Netherlands. [Bayer, A. D.] Karlsruhe Inst Technol, Inst Meteorol & Climate Res, Atmospher Environm Res, Kreuzeckbahnstr 19, D-82467 Garmisch Partenkirchen, Germany. [Bottcher, K.] Joint Res Ctr, Inst Sustainabil, Via E Fermi 2749, I-21027 Ispra, Italy. [Bottcher, K.] Finnish Environm Inst, Mechelininkatu 34 A, Helsinki 00251, Finland. [Brodsky, L.] Czech Univ Life Sci Prague, Dept Soil Sci & Soil Protect, Prague, Czech Republic. [Du, C. W.] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, East Beijing Rd 71, Nanjing 210008, Peoples R China. [Fouad, Y.; Walter, C.] Agrocampus Ouest, INRA, UMR SAS 1069, Rennes, France. [Genot, V.] Univ Liege, Gembloux Agrobio Tech Ulg GxaBt Belgium, Soil Sci Unit, Passage Deportes 2, B-5030 Gembloux, Belgium. [Gomez, C.] IRD, UMR LISAH, INRA, SupAgro, F-34060 Montpellier, France. [Grunwald, S.] Univ Florida, Soil & Water Sci Dept, Gainesville, FL 32611 USA. [Gubler, A.] Agroscope, Swiss Soil Monitoring Network NABO, Reckenholzstr 191, CH-8046 Zurich, Switzerland. [Guerrero, C.] Univ Miguel Hernandez Riche UMH, Dept Agroquim & Medio Ambiente, Ave Univ, E-03202 Elche, Spain. [Hedley, C. B.; Roudier, P.] Landcare Res, Private Bag 11052, Palmerston North 4442, New Zealand. [Knadel, M.] Aarhus Univ, Dept Agroecol, Blichers Alle 20,PO 50, DK-8830 Tjele, Denmark. [Morras, H. J. M.] INTA, CIRN, Inst Suelos, RA-1712 Castelar, Buenos Aires, Argentina. [Nocita, M.] Presence Earthcollect, POB 237, ZA-6335 Patensie, Eastern Cape, South Africa. [Ramirez-Lopez, L.] BUCHI Lab Tech AG, Meierseggstr 40, Flawil, Switzerland. [Rufasto Campos, E. M.] Univ Nacl Pedro Ruiz Gallo, Fac Agron, Dept Acad Suelos, Lambayeque, Peru. [Sanborn, P.] Univ No British Columbia, Ecosyst Sci & Management Program, 3333 Univ Way, Prince George, BC V2N 4Z9, Canada. [Sellitto, V. M.] Univ Molise, Dipartimento Agr Ambiente Alimenti DIAAA, V Sanctis, I-86100 Campobasso, CB, Italy. [Sudduth, K. A.] ARS, USDA, Cropping Syst & Water Qual Res Unit, Columbia, MO USA. [Rawlins, B. G.] British Geol Survey, Nottingham NG12 5GG, England. [Hong, S. Y.] Rural Dev Adm, Natl Inst Agr Sci, Wonju 55365, South Korea. RP Rossel, RAV (reprint author), CSIRO Land & Water, POB 1666, Canberra, ACT 2601, Australia. EM raphael.viscarra-rossel@csiro.au RI BERNOUX, Martial/B-3090-2008; Changwen, Du/E-5239-2013; Knadel, Maria/O-9886-2015; shi, Zhou/A-2283-2015; kiaie, robabeh/I-2157-2016; kiaie, fatemeh/I-6083-2016; UMR SAS, INRA/L-1751-2013; UMR SAS, MOsol/M-5051-2013; Gomez, Cecile/B-8473-2012; viscarra rossel, raphael/B-4061-2011; Guerrero, Cesar/A-3550-2008; Dematte, Jose/I-5990-2013; Brown, David/A-2002-2009; Walter, Christian/A-1015-2012 OI BERNOUX, Martial/0000-0002-2913-3590; Changwen, Du/0000-0002-9064-3581; Knadel, Maria/0000-0001-7539-6191; kiaie, robabeh/0000-0001-5251-3201; Gomez, Cecile/0000-0002-2986-430X; viscarra rossel, raphael/0000-0003-1540-4748; Guerrero, Cesar/0000-0002-3960-9724; Walter, Christian/0000-0002-4395-4942 NR 196 TC 7 Z9 7 U1 27 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0012-8252 EI 1872-6828 J9 EARTH-SCI REV JI Earth-Sci. Rev. PD APR PY 2016 VL 155 BP 198 EP 230 DI 10.1016/j.earscirev.2016.01.012 PG 33 WC Geosciences, Multidisciplinary SC Geology GA DK0TJ UT WOS:000374624800011 ER PT J AU Auger, J Meyer, SE Jenkins, SH AF Auger, Janene Meyer, Susan E. Jenkins, Stephen H. TI A mast-seeding desert shrub regulates population dynamics and behavior of its heteromyid dispersers SO ECOLOGY AND EVOLUTION LA English DT Article DE Blackbrush; Coleogyne ramosissima; Dipodomys ordii; interference competition; kangaroo rat; optimal caching theory; Perognathus flavescens; pocket mouse; resource abundance hypothesis; scatterhoarding ID RATS DIPODOMYS-MERRIAMI; KANGAROO RATS; SEEDLING ESTABLISHMENT; TERRITORY SIZE; SMALL MAMMALS; HOME-RANGE; RODENTS; RESPONSES; AVAILABILITY; CONSEQUENCES AB Granivorous rodent populations in deserts are primarily regulated through precipitation-driven resource pulses rather than pulses associated with mast-seeding, a pattern more common in mesic habitats. We studied heteromyid responses to mast-seeding in the desert shrub blackbrush (Coleogyne ramosissima), a regionally dominant species in the Mojave-Great Basin Desert transition zone. In a 5-year study at Arches National Park, Utah, USA, we quantified spatiotemporal variation in seed resources in mast and intermast years in blackbrush-dominated and mixed desert vegetation and measured responses of Dipodomys ordii (Ord's kangaroo rat) and Perognathus flavescens (plains pocket mouse). In blackbrush-dominated vegetation, blackbrush seeds comprised > 79% of seed production in a mast year, but 0% in the first postmast year. Kangaroo rat abundance in blackbrush-dominated vegetation was highest in the mast year, declined sharply at the end of the first postmast summer, and then remained at low levels for 3 years. Pocket mouse abundance was not as strongly associated with blackbrush seed production. In mixed desert vegetation, kangaroo rat abundance was higher and more uniform through time. Kangaroo rats excluded the smaller pocket mice from resource-rich patches including a pipeline disturbance and also moved their home range centers closer to this disturbance in a year of low blackbrush seed production. Home range size for kangaroo rats was unrelated to seed resource density in the mast year, but resource-poor home ranges were larger (P < 0.001) in the first postmast year, when resources were limiting. Blackbrush seeds are higher in protein and fat but lower in carbohydrates than the more highly preferred seeds of Indian ricegrass (Achnatherum hymenoides) and have similar energy value per unit of handling time. Kangaroo rats cached seeds of these two species in similar spatial configurations, implying that they were equally valued as stored food resources. Blackbrush mast is a key resource regulating populations of kangaroo rats in this ecosystem. C1 [Auger, Janene] Univ Nevada, Program Ecol Evolut & Conservat Biol, Reno, NV 89557 USA. [Meyer, Susan E.] USFS Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. [Jenkins, Stephen H.] Univ Nevada, Dept Biol, Reno, NV 89557 USA. RP Meyer, SE (reprint author), USFS Shrub Sci Lab, 735 North 500 East, Provo, UT 84606 USA. EM smeyer@fs.fed.us NR 60 TC 1 Z9 1 U1 10 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD APR PY 2016 VL 6 IS 8 BP 2275 EP 2296 DI 10.1002/ece3.2035 PG 22 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DJ9YD UT WOS:000374568300003 PM 27069574 ER PT J AU Yu, LJ Zhong, SY Pei, LS Bian, XD Heilman, WE AF Yu, Lejiang Zhong, Shiyuan Pei, Lisi Bian, Xindi Heilman, Warren E. TI Contribution of large-scale circulation anomalies to changes in extreme precipitation frequency in the United States SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE extreme precipitation; the Pacific Decadal Oscillation (PDO); the Atlantic Multidecadal Oscillation (AMO); interdecadal variability ID INTENSE PRECIPITATION; EXTENDED RECONSTRUCTION; CLIMATE-CHANGE; REAL-TIME; EVENTS; TRENDS; OSCILLATION; TEMPERATURE; RAINFALL; PACIFIC AB The mean global climate has warmed as a result of the increasing emission of greenhouse gases induced by human activities. This warming is considered the main reason for the increasing number of extreme precipitation events in the US. While much attention has been given to extreme precipitation events occurring over several days, which are usually responsible for severe flooding over a large region, little is known about how extreme precipitation events that cause flash flooding and occur at sub-daily time scales have changed over time. Here we use the observed hourly precipitation from the North American Land Data Assimilation System Phase 2 forcing datasets to determine trends in the frequency of extreme precipitation events of short (1 h, 3 h, 6 h, 12 h and 24 h) duration for the period 1979-2013. The results indicate an increasing trend in the central and eastern US. Over most of the western US, especially the Southwest and the Intermountain West, the trends are generally negative. These trends can be largely explained by the interdecadal variability of the Pacific Decadal Oscillation and Atlantic Multidecadal Oscillation (AMO), with the AMO making a greater contribution to the trends in both warm and cold seasons. C1 [Yu, Lejiang] Polar Res Inst China, Shanghai, Peoples R China. [Yu, Lejiang; Zhong, Shiyuan; Pei, Lisi] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. [Bian, Xindi; Heilman, Warren E.] US Forest Serv, USDA, No Res Stn, Lansing, MI USA. RP Yu, LJ (reprint author), Polar Res Inst China, Shanghai, Peoples R China.; Yu, LJ (reprint author), Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. EM yulj@msu.edu FU USDA Forest Service Northern Research Station [11-JV-11242306-065]; AgBio Research of Michigan State University FX This research was supported by the USDA Forest Service Northern Research Station under Joint Venture Agreement #11-JV-11242306-065 and by AgBio Research of Michigan State University. NR 40 TC 1 Z9 1 U1 12 U2 20 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD APR PY 2016 VL 11 IS 4 AR 044003 DI 10.1088/1748-9326/11/4/044003 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DL6JZ UT WOS:000375746800006 ER PT J AU Volpe, RJ Park, TA Dong, FX Jensen, HH AF Volpe, Richard J. Park, Timothy A. Dong, Fengxia Jensen, Helen H. TI Somatic cell counts in dairy marketing: quantile regression for count data SO EUROPEAN REVIEW OF AGRICULTURAL ECONOMICS LA English DT Article DE somatic cell counts; ARMS data; farm profitability; quantile regression; count data ID ENDEMIC LIVESTOCK DISEASES; MILK QUALITY; GREAT-BRITAIN; HERD SIZE; FARMS; MASTITIS; COSTS; MANAGEMENT; ONTARIO; REGION AB We study the determinants of somatic cell count (SCC) for farm milk among US dairies. We synthesise much of the work that has been done to model SCC determinants in order to identify the potential impacts of buyer-imposed penalties and incentives within the supply chain. Additionally, we estimate quantile regression for count data to measure impacts specifically for those operations with the highest SCC and to account for the statistical properties of the data. Premiums in particular have the potential to reduce SCC considerably where it is currently the highest. We draw implications for profitability in relation to SCC reduction. C1 [Volpe, Richard J.] Cal Poly, San Luis Obispo, CA USA. [Park, Timothy A.] USDA, Econ Res Serv, Washington, DC USA. [Dong, Fengxia] Univ Wisconsin, Madison, WI USA. [Jensen, Helen H.] Iowa State Univ, Ames, IA USA. RP Volpe, RJ (reprint author), Cal Poly, San Luis Obispo, CA USA. EM rvolpe@calpoly.edu OI Jensen, Helen/0000-0001-6878-1729 FU USDA Economic Research Service [58-4000-9-0049]; Iowa State University [58-4000-9-0049] FX The Economic Research Service, US Department of Agriculture (Washington, DC) provided access to the data, as well as partial financial support for the analysis under Cooperative Research Agreement 58-4000-9-0049 between USDA Economic Research Service and Iowa State University. The authors thank David Hennessy for helpful discussions at earlier stages of the research. We also thank the editor and two anonymous reviewers for their consideration and helpful comments and suggestions. The views expressed in this article are those of the authors and are not attributable to USDA or ERS. NR 42 TC 0 Z9 0 U1 6 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0165-1587 EI 1464-3618 J9 EUR REV AGRIC ECON JI Eur. Rev. Agric. Econ. PD APR PY 2016 VL 43 IS 2 BP 331 EP 358 DI 10.1093/erae/jbv021 PG 28 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DJ7TE UT WOS:000374414600006 ER PT J AU Muziasari, WI Parnanen, K Johnson, TA Lyra, C Karkman, A Stedtfeld, RD Tamminen, M Tiedje, JM Virta, M AF Muziasari, Windi I. Parnanen, Katariina Johnson, Timothy A. Lyra, Christina Karkman, Antti Stedtfeld, Robert D. Tamminen, Manu Tiedje, James M. Virta, Marko TI Aquaculture changes the profile of antibiotic resistance and mobile genetic element associated genes in Baltic Sea sediments SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE qPCR array; fish farms; environmental resistome; mobile genetic elements; transposons; Baltic Sea ID ANTIMICROBIAL RESISTANCE; HUMAN HEALTH; BACTERIA; ENVIRONMENT; WATER; IRRIGATION; FEED AB Antibiotics are commonly used in aquaculture and they can change the environmental resistome by increasing antibiotic resistance genes (ARGs). Sediment samples were collected from two fish farms located in the Northern Baltic Sea, Finland, and from a site outside the farms (control). The sediment resistome was assessed by using a highly parallel qPCR array containing 295 primer sets to detect ARGs, mobile genetic elements and the 16S rRNA gene. The fish farm resistomes were enriched in transposon and integron associated genes and in ARGs encoding resistance to antibiotics which had been used to treat fish at the farms. Aminoglycoside resistance genes were also enriched in the farm sediments despite the farms not having used aminoglycosides. In contrast, the total relative abundance values of ARGs were higher in the control sediment resistome and they were mainly genes encoding efflux pumps followed by beta-lactam resistance genes, which are found intrinsically in many bacteria. This suggests that there is a natural Baltic sediment resistome. The resistome associated with fish farms can be from native ARGs enriched by antibiotic use at the farms and/or from ARGs and mobile elements that have been introduced by fish farming. C1 [Muziasari, Windi I.; Parnanen, Katariina; Lyra, Christina; Karkman, Antti; Virta, Marko] Univ Helsinki, Dept Food & Environm Sci, POB 56, FIN-00014 Helsinki, Finland. [Johnson, Timothy A.] McMaster Univ, Inst Infect Dis Res, Dept Biochem & Biomed Sci, Hamilton, ON L8S 4L8, Canada. [Johnson, Timothy A.; Stedtfeld, Robert D.; Tiedje, James M.] Michigan State Univ, Dept Civil & Environm Engn, Ctr Microbial Ecol, E Lansing, MI 48824 USA. [Johnson, Timothy A.] USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Tamminen, Manu] ETH, Dept Environm Syst Sci, CH-8092 Zurich, Switzerland. [Tamminen, Manu] Eawag, Dept Aquat Ecol, CH-8600 Dubendorf, Switzerland. RP Virta, M (reprint author), Univ Helsinki, Dept Food & Environm Sci, POB 56, FIN-00014 Helsinki, Finland. EM marko.virta@helsinki.fi RI Virta, Marko/B-4292-2010; Parnanen, Katariina/P-5176-2016; OI Virta, Marko/0000-0001-5981-7566; Parnanen, Katariina/0000-0003-2220-1738; Karkman, Antti/0000-0003-0983-3319; Muziasari, Windi Indra/0000-0002-5542-4375 FU Maj and Tor Nessling foundation; Academy of Finland; Center for Microbial Ecology; Center for Health Impacts of Agriculture at Michigan State University FX The research was funded by Maj and Tor Nessling foundation and Academy of Finland grants and with support from the Center for Microbial Ecology and the Center for Health Impacts of Agriculture at Michigan State University. NR 38 TC 3 Z9 3 U1 21 U2 39 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0168-6496 EI 1574-6941 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD APR PY 2016 VL 92 IS 4 AR fiw052 DI 10.1093/femsec/fiw052 PG 7 WC Microbiology SC Microbiology GA DJ8RE UT WOS:000374479400017 PM 26976842 ER PT J AU Lange, MD Beck, BH Brown, JD Farmer, BD Barnett, LM Webster, CD AF Lange, Miles D. Beck, Benjamin H. Brown, Jason D. Farmer, Bradley D. Barnett, L. Matthew Webster, Carl D. TI Missing the target: DNAk is a dominant epitope in the humoral immune response of channel catfish (Ictalurus punctatus) to Flavobacterium columnare SO FISH & SHELLFISH IMMUNOLOGY LA English DT Article DE Channel catfish; Antibody response; Immunization; DNAk ID HEAT-SHOCK PROTEINS; MONOCLONAL-ANTIBODIES; LYMPHOCYTE HETEROGENEITY; MOONLIGHTING PROTEINS; AEROMONAS-HYDROPHILA; CUTANEOUS ANTIBODIES; INFECTIOUS-DISEASES; PROTEOMIC ANALYSIS; FISH; IDENTIFICATION AB Vaccination remains a viable alternative for bacterial disease protection in fish; however additional work is required to understand the mechanisms of adaptive immunity in the channel catfish. To assess the humoral immune response to Flavobacterium columnare; a group of channel catfish were first immunized with F. columnare LV-359-01 cultured in iron-depleted media, before being challenged with wild type F. columnare LV-359-01. The immunization protocol did not confer increased protection against F. columnare; however both control and immunized responders generated serum and skin IgM antibodies against F columnare proteins. Western blot analyses of individuals from both groups showed that IgM antibodies were generated to the same 70 kDa extracellular protein, which was identified to be the bacterial chaperonin protein DNAk. Antibodies generated were cross reactive to DNAk proteins found in other gram negative bacteria. Our data suggests that DNAk is the dominant epitope in the channel catfish B-cell response to F columnare. Published by Elsevier Ltd. C1 [Lange, Miles D.; Beck, Benjamin H.; Brown, Jason D.; Farmer, Bradley D.; Barnett, L. Matthew; Webster, Carl D.] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. [Beck, Benjamin H.] USDA ARS, Aquat Anim Hlth Res Unit, Auburn, AL USA. RP Lange, MD (reprint author), USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM miles.lange@ars.usda.gov FU USDA-ARS-CRIS [6225-32000-005-00D] FX The USDA is an equal opportunity provider and employer. We thank the University of Arkansas for Medical Sciences Proteomics Core for high-throughput mass spectrometry analysis. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. This study was funded under the USDA-ARS-CRIS project #6225-32000-005-00D. NR 57 TC 0 Z9 0 U1 3 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1050-4648 EI 1095-9947 J9 FISH SHELLFISH IMMUN JI Fish Shellfish Immunol. PD APR PY 2016 VL 51 BP 170 EP 179 DI 10.1016/j.fsi.2016.02.015 PG 10 WC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences GA DK0QQ UT WOS:000374617700020 PM 26892797 ER PT J AU Mathews, EA Jemison, LA Pendleton, GW Blejwas, KM Hood, KE Raum-Suryan, KL AF Mathews, Elizabeth A. Jemison, Lauri A. Pendleton, Grey W. Blejwas, Karen M. Hood, Kevin E. Raum-Suryan, Kimberly L. TI Haul-out patterns and effects of vessel disturbance on harbor seals (Phoca vitulina) on glacial ice in Tracy Arm, Alaska SO FISHERY BULLETIN LA English DT Article ID BAY NATIONAL-PARK; MATERNAL-BEHAVIOR; NUMBERS; PUPS; RICHARDSI; ABUNDANCE; OXYTOCIN; DECLINE; FJORD; RATS AB Tidewater glacial fjords provide important habitat for breeding harbor seals (Phoca vitulina) that rest, give birth, and nurse pups on icebergs. These fjords also attract tourist vessels that potentially disturb seals. In. May and June during 2001-2006, we documented seal abundance, pupping phenology, and seal vessel interactions in Tracy Arm, a glacial fjord in southeastern. Alaska. We used randomized observations to determine the frequency at which seals entered the water in the presence and absence of vessels, and we estimated the reaction distances of seals to approaching vessels. Mean daily vessel counts varied from 10.2 (2001) to 2.0 (2006) (range: 1-33). Tour and power vessels were the most common types of vessels, but seals were most sensitive to cruise ships and kayaks. The odds of a seal entering the water were higher when vessels were present (>2 times) or within 100 m (3.7 times), and when a pup was present (1.3 times). The baseline, undisturbed, rate of seals entering the water was 0.06 (95% CI: 0.05-0.08) per 10 min. Seal births occurred during 30 May-25 June and peaked (4-8 per day) during 7-13 June. The maximum pup count (408) was observed on 24 June. Harbor seal fitness in Tracy Arm may be reduced by vessel disturbances during breeding and pupping. C1 [Mathews, Elizabeth A.; Blejwas, Karen M.] Univ Alaska Southeast, Dept Nat Sci, 11120 Glacier Hwy, Juneau, AK 99801 USA. [Mathews, Elizabeth A.; Blejwas, Karen M.] 1350-B Yulupa Ave, Santa Rosa, CA 95405 USA. [Jemison, Lauri A.; Pendleton, Grey W.; Blejwas, Karen M.] Alaska Dept Fish & Game, Div Wildlife Conservat, POB 115525, Juneau, AK 99811 USA. [Hood, Kevin E.] US Forest Serv, Juneau Ranger Dist Wilderness Program, USDA, 8510 Mendenhall Loop Rd, Juneau, AK 99801 USA. [Raum-Suryan, Kimberly L.] Sea Gypsy Res, 928 NW Cottage St, Newport, OR 97365 USA. RP Mathews, EA (reprint author), Univ Alaska Southeast, Dept Nat Sci, 11120 Glacier Hwy, Juneau, AK 99801 USA.; Mathews, EA (reprint author), 1350-B Yulupa Ave, Santa Rosa, CA 95405 USA. EM BethAnnMathews@gmail.com FU NMFS; National Science Foundation FX This project was supported by NMFS grants to the Alaska Department of Fish and Game and by a National Science Foundation Research Experiences for Undergraduates Grant to the University of Alaska, Southeast. Logistical support was provided by the U.S. Forest Service (USFS), Juneau Ranger District, and by the captains of the MV Keet. B. Nielsen (Alaska Department of Fish and Game), T. Lydon and J. Neary (USFS) provided field leadership, and we thank the many field assistants for careful data collection. Research was conducted under NMFS research permits 358-1585 and 358-1787. NR 54 TC 0 Z9 0 U1 6 U2 7 PU NATL MARINE FISHERIES SERVICE SCIENTIFIC PUBL OFFICE PI SEATTLE PA 7600 SAND POINT WAY NE BIN C15700, SEATTLE, WA 98115 USA SN 0090-0656 EI 1937-4518 J9 FISH B-NOAA JI Fish. Bull. PD APR PY 2016 VL 114 IS 2 BP 186 EP 202 DI 10.7755/FB.114.2.6 PG 17 WC Fisheries SC Fisheries GA DK2DF UT WOS:000374724100006 ER PT J AU Liu, PW Bongiovanni, T Monsivais-Huertero, A Judge, J Steele-Dunne, S Bindlish, R Jackson, TJ AF Liu, Pang-Wei Bongiovanni, Tara Monsivais-Huertero, Alejandro Judge, Jasmeet Steele-Dunne, Susan Bindlish, Rajat Jackson, Thomas J. TI Assimilation of Active and Passive Microwave Observations for Improved Estimates of Soil Moisture and Crop Growth SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Active microwave remote sensing; aquarius; data assimilation; passive microwave remote sensing; soil moisture (SM); soil moisture active passive (SMAP) ID DYNAMIC VEGETATION; SOYBEAN MODEL; L-BAND; BACKSCATTERING MODEL; C-BAND; RADAR; YIELD; EMISSION; COEFFICIENTS; CALIBRATION AB An ensemble Kalman Filter-based data assimilation framework that links a crop growth model with active and passive (AP) microwave models was developed to improve estimates of soil moisture (SM) and vegetation biomass over a growing season of soybean. Complementarities in AP observations were incorporated to the framework, where the active observations were used to optimize surface roughness and update vegetation biomass, while passive observations were used to update SM. The framework was implemented in a rain-fed agricultural region of the southern La-Plata Basin during the 2011-2012 growing season, through a synthetic experiment and AP observations from the Aquarius mission. The synthetic experiment was conducted at a temporal resolution of 3 and 7 days to match the current AP missions. The assimilated estimates of SM in the root zone and dry biomass were improved compared to those from the cases without assimilation, during both 3- and 7-day assimilation scenarios. Particularly, the 3-day assimilation provided the best estimates of SM in the near surface and dry biomass with reductions in RMSEs of 41% and 42%, respectively. The absolute differences of assimilated LAI from Aquarius were < 0.29 compared to the MODIS LAI indicating that the performance of assimilation was similar to the MODIS product at a regional scale. This study demonstrates the potential of assimilation using AP observations at high temporal resolution such as those from soil moisture active passive (SMAP) for improved estimates of SM and vegetation parameters. C1 [Liu, Pang-Wei; Bongiovanni, Tara; Judge, Jasmeet] Univ Florida, Inst Food & Agr Sci, Dept Agr & Biol Engn, Ctr Remote Sensing, Gainesville, FL 32611 USA. [Monsivais-Huertero, Alejandro] Natl Polytech Inst, ESIME Ticoman CDA, Mexico City 07738, DF, Mexico. [Steele-Dunne, Susan] Delft Univ Technol, Dept Water Management, NL-2628 Delft, Netherlands. [Bindlish, Rajat; Jackson, Thomas J.] USDA, Agr Res Ctr, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Bongiovanni, T (reprint author), Univ Florida, Inst Food & Agr Sci, Dept Agr & Biol Engn, Ctr Remote Sensing, Gainesville, FL 32611 USA. EM bonwei@ufl.edu OI Liu, Pang-Wei/0000-0002-3789-594X FU NASA-Terrestrial Hydrology Program [THP-NNX09AK29G]; Netherlands Organization for Scientific Research (NWO) Veni Grant Program [ALW 863.09.015]; National Council of Science and Technology of Mexico [CB-2010-155375] FX This work was supported by the NASA-Terrestrial Hydrology Program (THP-NNX09AK29G), in part by the Netherlands Organization for Scientific Research (NWO) Veni Grant Program (ALW 863.09.015), and in part by the National Council of Science and Technology of Mexico (CB-2010-155375). NR 66 TC 0 Z9 0 U1 13 U2 21 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD APR PY 2016 VL 9 IS 4 BP 1357 EP 1369 DI 10.1109/JSTARS.2015.2506504 PG 13 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA DL8DA UT WOS:000375868800004 ER PT J AU Liao, TH Kim, SB Tan, SR Tsang, L Su, CX Jackson, TJ AF Liao, Tien-Hao Kim, Seung-Bum Tan, Shurun Tsang, Leung Su, Chenxin Jackson, Thomas J. TI Multiple Scattering Effects With Cyclical Correction in Active Remote Sensing of Vegetated Surface Using Vector Radiative Transfer Theory SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Backscattering enhancement; cyclical correction; multiple scattering effects; remote sensing; soil moisture; vector radiative transfer ID BACKSCATTERING ENHANCEMENT; SOIL; MODEL; TERRAIN; RADAR; LAYER AB The energy transport in a vegetated (corn) surface layer is examined by solving the vector radiative transfer equation using a numerical iterative approach. This approach allows a higher order that includes the multiple scattering effects. Multiple scattering effects are important when the optical thickness and scattering albedo of the vegetation layer are large. When both the albedo and the optical thickness exceed 0.4, higher orders contribute significantly (e.g., vertical polarization at L-band). The approach is applied to vegetated surfaces using typical crop structure for backscattering from L-band to Ku-band. For corn fields at L-band, multiple scattering effects are more important for vertical scattered wave with vertical incidence (VV). For example, when vegetation water content (VWC) is 3kg/m(2), the deviation between first order and multiple scattering for corn field for VV could be 3.5 dB while 0.7 dB for horizontal scattered wave with horizontal incidence (HH). The iterative approach also allows the separation of the contribution to backscattering from each scattering order and scattering mechanism. Each scattering mechanism is associated with a unique scattering path. By examining the duality of the paths, we are able to identify the cyclical terms with existence of a reflective boundary. The cyclical correction to the backscattering accounts for backscattering enhancement effects on the copolarization by a factor of two. The approach is validated against the SMAPVEX12 L-band corn dataset over the entire crop growth and large soil moisture variations. The model prediction matches the observation with 1.93 and 1.46 dB root-mean-square error (RMSE) for VV and HH, respectively, while correlations are 0.67 and 0.88, respectively. Time-series retrieval is also applied successfully for both soil moisture and VWC with 0.06 cm(3)/cm(3) and 0.44 kg/m(2) RMSE, respectively, while correlations are 0.7 and 0.92, respectively. For large VWC, this approach corrects the underestimated backscatters in the single scattering caused by large attenuation. C1 [Liao, Tien-Hao; Tan, Shurun; Tsang, Leung] Univ Michigan, Dept Elect Engn & Comp Sci, Ann Arbor, MI 48109 USA. [Kim, Seung-Bum] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. [Su, Chenxin] Univ Washington, Elect Engn, Seattle, WA 98195 USA. [Jackson, Thomas J.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Liao, TH (reprint author), Univ Michigan, Dept Elect Engn & Comp Sci, Ann Arbor, MI 48109 USA. EM thliao@uw.edu FU NASA SMAP; National Aeronautics and Space Administration FX The authors would like to thank NASA SMAP project to support the work and the SMAPVEX12 team to provide the measurement data. The research described in this publication was carried out in part at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. Surface scattering results from NMM3D were computed using NSF XSEDE facility. NR 24 TC 1 Z9 1 U1 1 U2 4 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD APR PY 2016 VL 9 IS 4 BP 1414 EP 1429 DI 10.1109/JSTARS.2015.2505638 PG 16 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA DL8DA UT WOS:000375868800008 ER PT J AU Jondiko, TO Yang, LY Hays, DB Ibrahim, AMH Tilley, M Awika, JM AF Jondiko, Tom O. Yang, Liyi Hays, Dirk B. Ibrahim, Amir M. H. Tilley, Michael Awika, Joseph M. TI Prediction of wheat tortilla quality using multivariate modeling of kernel, flour, and dough properties SO INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES LA English DT Article DE Wheat quality; Tortilla; Flatbread; Gluten; Genetic improvement ID PROTEIN-FRACTIONS; STARCH; FUNCTIONALITY; QUANTITATION; GLUTEN; SIZE AB Traditional wheat quality methods for bread have poor predictive power for flatbread quality, which impedes genetic improvement of wheat for the growing market. We used a multivariate discriminant analysis to predict tortilla quality using a set of 16 variables derived from kernel properties, flour composition, and dough theological properties of 187 experimental hard wheat samples grown across Texas. A discriminant rule (suitability for tortillas = diameter > 165 mm + day 16 flexibility score > 3.0) was used to classify samples. Multivariate normal distribution of the data was established (Shapiro-Wilk p > 0.05). Logistic regression and stepwise variable selection identified an optimum model comprising kernel weight, glutenin-gliadin ratio, insoluble polymeric proteins, and dough extensibility and stress relaxation parameters, as the most important variables. Cross-validation indicated 83% model prediction efficiency. This work provides important insight on potential targets for wheat quality genetic improvement for tortillas and specialty product market. Industrial relevance: Tortillas and other flatbread manufacturers currently use wheat developed for other commodities and rely on trial and error, and use of various additives to optimize product quality. Genetic development of wheat for these markets is impeded by lack of knowledge of specific grain quality parameters to target. With the growing demand for clean label and healthy offerings by consumers, the industry is looking for natural ingredients with improved functionality. This work provides the first insight into the specific wheat composition and functional parameters that can predict tortilla quality. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Jondiko, Tom O.; Yang, Liyi; Hays, Dirk B.; Ibrahim, Amir M. H.; Awika, Joseph M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Jondiko, Tom O.; Yang, Liyi; Awika, Joseph M.] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA. [Tilley, Michael] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. RP Awika, JM (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM jawika@ag.tamu.edu FU Texas Wheat Producers Board; Texas A&M AgriLife Research FX We thank the Texas Wheat Producers Board, and the Texas A&M AgriLife Research for financial support. NR 26 TC 0 Z9 0 U1 3 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1466-8564 EI 1878-5522 J9 INNOV FOOD SCI EMERG JI Innov. Food Sci. Emerg. Technol. PD APR PY 2016 VL 34 BP 9 EP 15 DI 10.1016/j.ifset.2016.01.010 PG 7 WC Food Science & Technology SC Food Science & Technology GA DL7IM UT WOS:000375814400002 ER PT J AU Chen, GQ van Erp, H Martin-Moreno, J Johnson, K Morales, E Browse, J Eastmond, PJ Lin, JT AF Chen, Grace Q. van Erp, Harrie Martin-Moreno, Jose Johnson, Kumiko Morales, Eva Browse, John Eastmond, Peter J. Lin, Jiann-Tsyh TI Expression of Castor LPAT2 Enhances Ricinoleic Acid Content at the sn-2 Position of Triacylglycerols in Lesquerella Seed SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE lesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering ID HYDROXY FATTY-ACIDS; PHYSARIA-FENDLERI OIL; TRANSGENIC ARABIDOPSIS; RICINUS-COMMUNIS; LYSOPHOSPHATIDYL ACYLTRANSFERASE; DIACYLGLYCEROL ACYLTRANSFERASE; OLEATE 12-HYDROXYLASE; LIMNANTHES-DOUGLASII; ENZYMATIC-SYNTHESIS; COCONUT ENDOSPERM AB Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18: 1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18: 1OH at the sn-2 position of TAGs from 2% to 14%-17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%-14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production. C1 [Chen, Grace Q.; Johnson, Kumiko; Morales, Eva; Lin, Jiann-Tsyh] USDA, Western Reg Res Ctr, ARS, 800 Buchanan St, Albany, CA 94710 USA. [van Erp, Harrie; Browse, John] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA. [van Erp, Harrie; Martin-Moreno, Jose; Eastmond, Peter J.] Rothamsted Res, Dept Plant Biol & Crop Sci, Harpenden AL5 2JQ, Herts, England. RP Chen, GQ (reprint author), USDA, Western Reg Res Ctr, ARS, 800 Buchanan St, Albany, CA 94710 USA. EM grace.chen@ars.usda.gov; harrie.van-erp@rothamsted.ac.uk; jmartinm83@gmail.com; kumiko.johnson@ars.usda.gov; evadesalta@gmail.com; jab@wsu.edu; peter.eastmond@rothamsted.ac.uk; jiann.lin@ars.usda.gov OI Eastmond, Peter/0000-0003-4526-5136 FU U.S. Department of Agriculture Current Research Information System Project [2030-21410-020-00D]; U.S. National Science Foundation [IOS-1339385]; UK Biotechnology and Biological Sciences Research Council [BBS/E/C/00005207] FX This study was supported by the U.S. Department of Agriculture Current Research Information System Project 2030-21410-020-00D (to Grace Q. Chen), the U.S. National Science Foundation grant IOS-1339385 (to John Browse) and the UK Biotechnology and Biological Sciences Research Council through Grant BBS/E/C/00005207 (to Peter Eastmond). USDA is an equal opportunity provider and employer. Mention of a specific product name by the U.S. Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 69 TC 0 Z9 0 U1 6 U2 11 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD APR PY 2016 VL 17 IS 4 AR 507 DI 10.3390/ijms17040507 PG 14 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DK0EL UT WOS:000374585300086 PM 27058535 ER PT J AU Kc, L Johnson, AK Shepherd, A Stinn, JP Xin, H Stalder, KJ Karriker, A Sutherland, MA Lay, DC Millman, ST AF Kc, L. Johnson, A. K. Shepherd, A. Stinn, J. P. Xin, H. Stalder, K. J. Karriker, A. Sutherland, M. A. Lay, D. C., Jr. Millman, S. T. TI Aversion to carbon dioxide gas in pigs using approach-avoidance and conditioned place avoidance paradigms SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE aversion testing; carbon dioxide; weaned pigs C1 [Kc, L.; Johnson, A. K.; Shepherd, A.; Stinn, J. P.; Xin, H.; Millman, S. T.] Iowa State Univ, Ames, IA USA. [Stalder, K. J.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Karriker, A.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Swine Med Educ Ctr, Ames, IA USA. [Sutherland, M. A.] AgResearch, Hamilton, New Zealand. [Lay, D. C., Jr.] USDA, W Lafayette, IN USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 020 BP 9 EP 10 DI 10.2527/msasas2016-020 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900021 ER PT J AU Marini, JC Agarwal, U Didelija, IC Stoll, B Burrin, DG AF Marini, J. C. Agarwal, U. Didelija, I. C. Stoll, B. Burrin, D. G. TI Citrulline and de novo arginine synthesis in perinatal and young pigs. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE arginine; citrulline; neonate; pig; tracer C1 [Marini, J. C.; Agarwal, U.; Didelija, I. C.; Stoll, B.; Burrin, D. G.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Marini, J. C.] Baylor Coll Med, Sect Crit Care Med, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 041 BP 18 EP 19 DI 10.2527/msasas2016-041 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900041 ER PT J AU Danielson, AM Azarpajouh, S Baker, RB Rademacher, CJ Dickson, JS Johnson, AK Karriker, LA Bigelow, TT Stalder, KJ AF Danielson, A. M. Azarpajouh, S. Baker, R. B. Rademacher, C. J. Dickson, J. S. Johnson, A. K. Karriker, L. A. Bigelow, T. T. Stalder, K. J. TI Enterobacteriaceae counts from livestock truck wash stations offering different service levels located in Iowa. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE biosecurity; trailer; truck wash C1 [Danielson, A. M.; Azarpajouh, S.; Dickson, J. S.; Stalder, K. J.] Iowa State Univ, Dept Anim Sci, Ames, IA USA. [Baker, R. B.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Rademacher, C. J.; Karriker, L. A.] Swine Med Educ Ctr, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Johnson, A. K.] Iowa State Univ, Ames, IA USA. [Bigelow, T. T.] USDA, APHIS, Des Moines, IA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 077 BP 36 EP 36 DI 10.2527/msasas2016-077 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900077 ER PT J AU Carroll, JA Sanchez, NCB Broadway, PR AF Carroll, J. A. Sanchez, N. C. Burdick Broadway, P. R. TI Exploring connections between metabolic profiles, stress responses, and immune function SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE immunity; metabolic profiles; stress C1 [Carroll, J. A.; Sanchez, N. C. Burdick; Broadway, P. R.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 092 BP 42 EP 43 DI 10.2527/msasas2016-092 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900092 ER PT J AU Northrop, EJ Rich, JJ Cushman, RA Perry, GA AF Northrop, E. J. Rich, J. J. Cushman, R. A. Perry, G. A. TI Impact of elevated preovulatory estradiol during a fixed-time AI protocol on uterine environment and embryonic survival to Day 16 SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE embryonic survival; estradiol; uterine environment C1 [Northrop, E. J.; Rich, J. J.; Perry, G. A.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. [Cushman, R. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 098 BP 45 EP 46 DI 10.2527/msasas2016-098 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900098 ER PT J AU Gentry, WW Weiss, CP Meredith, CM Brauer, CL McCollum, FT Cole, NA Jennings, JS AF Gentry, W. W. Weiss, C. P. Meredith, C. M. Brauer, C. L. McCollum, F. T. Cole, N. A. Jennings, J. S. TI Effects of treating sorghum wet distillers grains plus solubles with calcium hydroxide in steam-flaked corn-based finishing diets on dry matter intake and ruminal fermentation characteristics SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE calcium hydroxide; fermentation; sorghum wet distillers grains plus solubles C1 [Gentry, W. W.; Weiss, C. P.; Meredith, C. M.; Brauer, C. L.; McCollum, F. T.; Jennings, J. S.] Texas A&M AgriLife Res & Extens Ctr, Amarillo, TX USA. [Cole, N. A.] USDA ARS, Bushland, TX USA. NR 0 TC 0 Z9 0 U1 2 U2 6 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 103 BP 48 EP 48 DI 10.2527/msasas2016-103 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900103 ER PT J AU Arkfeld, EK Mohrhauser, DA King, DA Wheeler, TL Dilger, AC Shackelford, SD Boler, DD AF Arkfeld, E. K. Mohrhauser, D. A. King, D. A. Wheeler, T. L. Dilger, A. C. Shackelford, S. D. Boler, D. D. TI Impact of sex on composition and quality of fresh loins, bellies, and fresh and processed hams SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE pork; sex; variability C1 [Arkfeld, E. K.; Dilger, A. C.; Boler, D. D.] Univ Illinois, Urbana, IL 61801 USA. [Mohrhauser, D. A.] Smithfield Foods, Denison, IA USA. [King, D. A.; Wheeler, T. L.; Shackelford, S. D.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 109 BP 51 EP 51 DI 10.2527/msasas2016-109 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900109 ER PT J AU Dunkelberger, JR Serao, NVL Kerrigan, MA Lunney, JK Rowland, RRR Dekkers, JCM AF Dunkelberger, J. R. Serao, N. V. L. Kerrigan, M. A. Lunney, J. K. Rowland, R. R. R. Dekkers, J. C. M. TI Genetic parameters and genomic regions associated with piglet response to vaccination for porcine reproductive and respiratory syndrome (PRRS) virus and co-infection with PRRS virus and porcine circovirus type 2b (PCV2b) SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE disease; GWAS; swine C1 [Dunkelberger, J. R.; Serao, N. V. L.; Dekkers, J. C. M.] Iowa State Univ, Ames, IA USA. [Serao, N. V. L.] N Carolina State Univ, Raleigh, NC 27695 USA. [Kerrigan, M. A.; Rowland, R. R. R.] Kansas State Univ, Manhattan, KS 66506 USA. [Lunney, J. K.] ARS, USDA, BARC, APDL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 112 BP 52 EP 53 DI 10.2527/msasas2016-112 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900112 ER PT J AU Arkfeld, EK Wilson, KB Overholt, MF Harsh, BN Lowell, JE Hogan, EK Klehm, BJ Bohrer, BM Peterson, BC Kroscher, KA Stites, CR Mohrhauser, DA King, DA Wheeler, TL Dilger, AC Shackelford, SD Boler, DD AF Arkfeld, E. K. Wilson, K. B. Overholt, M. F. Harsh, B. N. Lowell, J. E. Hogan, E. K. Klehm, B. J. Bohrer, B. M. Peterson, B. C. Kroscher, K. A. Stites, C. R. Mohrhauser, D. A. King, D. A. Wheeler, T. L. Dilger, A. C. Shackelford, S. D. Boler, D. D. TI Effect of marketing group on the variability of fresh loin, belly, and fresh and processed ham quality from pigs sourced from a commercial processing facility. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE composition; marketing group; quality C1 [Arkfeld, E. K.; Wilson, K. B.; Overholt, M. F.; Harsh, B. N.; Lowell, J. E.; Hogan, E. K.; Klehm, B. J.; Bohrer, B. M.; Peterson, B. C.; Kroscher, K. A.; Stites, C. R.; Dilger, A. C.; Boler, D. D.] Univ Illinois, Urbana, IL 61801 USA. [Mohrhauser, D. A.] Smithfield Foods, Denison, IA USA. [King, D. A.; Wheeler, T. L.; Shackelford, S. D.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 129 BP 60 EP 61 DI 10.2527/msasas2016-129 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900129 ER PT J AU King, DA Shackelford, SD Wheeler, TL AF King, D. A. Shackelford, S. D. Wheeler, T. L. TI Intramuscular variation in fresh ham muscle color. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE color; fiber-type; myoglobin C1 [King, D. A.; Shackelford, S. D.; Wheeler, T. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 131 BP 61 EP 61 DI 10.2527/msasas2016-131 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900131 ER PT J AU Shackelford, SD King, DA Wheeler, TL AF Shackelford, S. D. King, D. A. Wheeler, T. L. TI Use of visible and near infrared spectroscopy to predict beef and pork quality. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Near-Infrared; Prediction; Tenderness C1 [Shackelford, S. D.; King, D. A.; Wheeler, T. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 137 BP 64 EP 64 DI 10.2527/msasas2016-137 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900137 ER PT J AU Ramsay, TG Blomberg, LA Vallet, JL Caperna, TJ AF Ramsay, T. G. Blomberg, L. A. Vallet, J. L. Caperna, T. J. TI Use of plasma orosomucoid in newborn piglets to predict preweaning growth performance and its potential mechanism of action. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE alpha 1-acid glycoprotein; biomarker; growth; neonate; orosomucoid C1 [Ramsay, T. G.; Blomberg, L. A.; Caperna, T. J.] ARS, USDA, BARC, Beltsville, MD USA. [Vallet, J. L.] ARS, USDA, US MARC, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 146 BP 68 EP 68 DI 10.2527/msasas2016-146 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900146 ER PT J AU Arkfeld, EK Shackelford, SD Dilger, AC Boler, DD AF Arkfeld, E. K. Shackelford, S. D. Dilger, A. C. Boler, D. D. TI Characterization of variability in the US pork supply. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE pork composition; quality; variability C1 [Arkfeld, E. K.; Dilger, A. C.; Boler, D. D.] Univ Illinois, Urbana, IL 61801 USA. [Shackelford, S. D.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 218 BP 103 EP 103 DI 10.2527/msasas2016-218 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900218 ER PT J AU Sanchez, NCB Carroll, JA Broadway, PR Yeater, KM De Rodas, B Brown, D Lawhon, SD AF Sanchez, N. C. Burdick Carroll, J. A. Broadway, P. R. Yeater, K. M. De Rodas, B. Brown, D. Lawhon, S. D. TI Oral Salmonella challenge alters feed preference in nursery pigs. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE feed preference; pigs; Salmonella C1 [Sanchez, N. C. Burdick; Carroll, J. A.; Broadway, P. R.] ARS, USDA, Livestock Issues Res Unit, Lubbock, TX USA. [Yeater, K. M.] ARS, USDA, Plains Area, Ft Collins, CO USA. [De Rodas, B.] Land OLakes, Gray Summit, MO USA. [Brown, D.] Purina Anim Nutr Ctr, Gray Summit, MO USA. [Lawhon, S. D.] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX USA. RI Lawhon, Sara/G-5147-2011 OI Lawhon, Sara/0000-0001-9154-8909 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 252 BP 119 EP 119 DI 10.2527/msasas2016-252 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900252 ER PT J AU Lindblom, SC Shurson, GC Dozier, WA Kerr, BJ AF Lindblom, S. C. Shurson, G. C. Dozier, W. A. Kerr, B. J. TI Digestibility of energy and lipids, and metabolic oxidation status in nursery pigs fed various lipids. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE digestibility; energy; lipids; pigs C1 [Lindblom, S. C.] Iowa State Univ, Ames, IA USA. [Shurson, G. C.] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. [Dozier, W. A.] Auburn Univ, Auburn, AL 36849 USA. [Kerr, B. J.] ARS, USDA, Ames, IA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 253 BP 120 EP 120 DI 10.2527/msasas2016-253 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900253 ER PT J AU Oliver, WT Wells, JE AF Oliver, W. T. Wells, J. E. TI Lysozyme as an alternative to antibiotics in swine feed. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Lysozyme; antibiotics; swine; gastrointestinal; microbiota C1 [Oliver, W. T.; Wells, J. E.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 302 BP 141 EP 142 DI 10.2527/msasas2016-302 PG 3 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900301 ER PT J AU Broadway, PR Carroll, JA Sanchez, NCB Bass, BE Frank, JW AF Broadway, P. R. Carroll, J. A. Sanchez, N. C. Burdick Bass, B. E. Frank, J. W. TI Supplementation of a Lactobacillus acidophilus fermentation product can attenuate the acute phase response following a lipopolysaccharide challenge in pigs. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE acute phase response; Lactobacillus acidophilus fermentation product; lipopolysaccharide C1 [Broadway, P. R.; Carroll, J. A.; Sanchez, N. C. Burdick] USDA ARS, Livestock Issues Res Unit, Lubbock, TX USA. [Bass, B. E.; Frank, J. W.] Diamond V, Cedar Rapids, IA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 307 BP 144 EP 144 DI 10.2527/msasas2016-307 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900306 ER PT J AU Soares, M Cushman, RA Northrop, EJ Larimore, EL Perry, GA AF Soares, M. Cushman, R. A. Northrop, E. J. Larimore, E. L. Perry, G. A. TI Relationship between ovarian reserve and preovulatory estradiol during a fixed-time AI protocol in beef heifers. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Fertility; Ovarian Reserve; Estradiol C1 [Soares, M.] Univ Fed Santa Maria, BR-97119900 Santa Maria, RS, Brazil. [Cushman, R. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Northrop, E. J.; Perry, G. A.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. [Larimore, E. L.] S Dakota State Univ, Brookings, SD 57007 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 315 BP 148 EP 148 DI 10.2527/msasas2016-315 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900314 ER PT J AU Cushman, RA Soares, M Abedal-Majed, M Cupp, AS Perry, GA Freetly, HC AF Cushman, R. A. Soares, M. Abedal-Majed, M. Cupp, A. S. Perry, G. A. Freetly, H. C. TI Fetal and postnatal nutritional programming of reproductive performance in ruminants. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Nutrition; epigenetics; reproduction C1 [Cushman, R. A.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Soares, M.] Univ Fed Santa Maria, BR-97119900 Santa Maria, RS, Brazil. [Abedal-Majed, M.; Cupp, A. S.] Univ Nebraska, Lincoln, NE USA. [Perry, G. A.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 322 BP 151 EP 151 DI 10.2527/msasas2016-322 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900321 ER PT J AU Desaulniers, AT Cederberg, RA Mills, GA Lents, CA White, BR AF Desaulniers, A. T. Cederberg, R. A. Mills, G. A. Lents, C. A. White, B. R. TI Gonadotropin-releasing hormone II receptor (GnRHR-II) knockdown reduces testis size and decreases testosterone secretion during pubertal development in swine. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE GnRH-II; GnRHR-II; Swine C1 [Desaulniers, A. T.; Cederberg, R. A.; Mills, G. A.; White, B. R.] Univ Nebraska, Lincoln, NE USA. [Lents, C. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 BP 153 EP 154 DI 10.2527/msasas2016-327 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900326 ER PT J AU Johnson, JS Martin, KL Stewart, KR AF Johnson, J. S. Martin, K. L. Stewart, K. R. TI Rapid fluctuations in ambient temperature before conception reduce fetal viability in replacement gilts. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE pigs; fetal viability; heat stress; temperature fluctuations C1 [Johnson, J. S.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Martin, K. L.; Stewart, K. R.] Purdue Univ, W Lafayette, IN 47907 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 326 BP 153 EP 153 DI 10.2527/msasas2016-326 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900325 ER PT J AU Cushman, RA Tenley, SC Soares, M McNeel, AK Gomes, RS Tait, RG Cupp, AS Kuehn, LA Snelling, WM Thallman, RM Perry, GA Chase, CC AF Cushman, R. A. Tenley, S. C. Soares, M. McNeel, A. K. Gomes, R. S. Tait, R. G., Jr. Cupp, A. S. Kuehn, L. A. Snelling, W. M. Thallman, R. M. Perry, G. A. Chase, C. C., Jr. TI Use of an electronic activity detection system to characterize estrus activity in crossbred beef heifers differing in follicle number. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Antral Follicle Count; Estrus; Estrous Cycle C1 [Cushman, R. A.; McNeel, A. K.; Tait, R. G., Jr.; Kuehn, L. A.; Snelling, W. M.; Thallman, R. M.; Chase, C. C., Jr.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Tenley, S. C.; Gomes, R. S.; Cupp, A. S.] Univ Nebraska, Lincoln, NE USA. [Soares, M.] Univ Fed Santa Maria, BR-97119900 Santa Maria, RS, Brazil. [Perry, G. A.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 332 BP 156 EP 156 DI 10.2527/msasas2016-332 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900331 ER PT J AU Weiss, CP Gentry, WW Brauer, CL McCollum, FT Cole, NA Jennings, JS AF Weiss, C. P. Gentry, W. W. Brauer, C. L. McCollum, F. T. Cole, N. A. Jennings, J. S. TI Effects of feeding corn condensed distillers solubles and crude glycerin alone or in combination on growth performance and carcass characteristics of finishing steers. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE wet distillers grains; condensed distillers solubles; crude glycerin C1 [Weiss, C. P.; Gentry, W. W.; Brauer, C. L.; McCollum, F. T.; Jennings, J. S.] Texas A&M AgriLife Res & Extens Ctr, Amarillo, TX USA. [Cole, N. A.] ARS, USDA, Bushland, TX USA. NR 0 TC 1 Z9 1 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 374 BP 175 EP 175 DI 10.2527/msasas2016-374 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900373 ER PT J AU Cox, JL Hales, KE Ulmer, KM Rasby, RJ Shackelford, SD Freetly, HC Drewnoski, ME AF Cox, J. L. Hales, K. E. Ulmer, K. M. Rasby, R. J. Shackelford, S. D. Freetly, H. C. Drewnoski, M. E. TI Effect of backgrounding system on feedlot performance and carcass characteristics of beef steers. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Backgrounding; Brassicas; Carcass quality; Corn residue; Feedlot C1 [Cox, J. L.; Ulmer, K. M.; Rasby, R. J.; Drewnoski, M. E.] Univ Nebraska, Dept Anim Sci, Lincoln, NE USA. [Hales, K. E.; Shackelford, S. D.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 380 BP 178 EP 178 DI 10.2527/msasas2016-380 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900379 ER PT J AU Myer, PR Wells, JE Smith, TPL Kuehn, LA Freetly, HC AF Myer, P. R. Wells, J. E. Smith, T. P. L. Kuehn, L. A. Freetly, H. C. TI Gut bacterial communities and their association with production parameters in beef cattle. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE feed efficiency; microbiome; 16S rRNA C1 [Myer, P. R.] Univ Tennessee, Inst Agr, Knoxville, TN 37901 USA. [Wells, J. E.; Smith, T. P. L.; Kuehn, L. A.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 392 BP 183 EP 184 DI 10.2527/msasas2016-392 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900391 ER PT J AU McClure, MW Cushman, RA Nonneman, DJ Lents, CA AF McClure, M. W. Cushman, R. A. Nonneman, D. J. Lents, C. A. TI Effects of neonatal litter size and age on ovarian gene expression and follicular development in gilts SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE pig; ovary; litter size; development; follicle; gene expression C1 [McClure, M. W.] Univ Tennessee, Martin, TN 38238 USA. [Cushman, R. A.; Nonneman, D. J.; Lents, C. A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 403 BP 188 EP 189 DI 10.2527/msasas2016-403 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900402 ER PT J AU Quail, LK Cushman, RA Perry, GA AF Quail, L. K. Cushman, R. A. Perry, G. A. TI Impact of initiating a fixed-time AI program on herd calving date and weaning weight. SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE Fixed-time AI; Reproductive efficiency; Calving distribution C1 [Quail, L. K.] S Dakota State Univ, Brookings, SD 57007 USA. [Cushman, R. A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Perry, G. A.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 401 BP 188 EP 188 DI 10.2527/msasas2016-401 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900400 ER PT J AU Zarek, CM Lindholm-Perry, AK Freetly, HC AF Zarek, C. M. Lindholm-Perry, A. K. Freetly, H. C. TI Differential expression of genes in the liver associated with gain and intake in beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE differential gene expression C1 [Zarek, C. M.; Lindholm-Perry, A. K.; Freetly, H. C.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 406 BP 190 EP 190 DI 10.2527/msasas2016-406 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900405 ER PT J AU Shade, KA Stewart, KR Johnson, JS AF Shade, K. A. Stewart, K. R. Johnson, J. S. TI Characterizing body temperature and movement differences at the onset of estrus in replacement gilts SO JOURNAL OF ANIMAL SCIENCE LA English DT Meeting Abstract DE pigs; estrus detection; temperature; movement C1 [Shade, K. A.; Johnson, J. S.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Stewart, K. R.] Purdue Univ, W Lafayette, IN 47907 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 SU 2 MA 415 BP 194 EP 194 DI 10.2527/msasas2016-415 PG 1 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XR UT WOS:000375783900414 ER PT J AU Galyean, ML Cole, NA Tedeschi, LO Branine, ME AF Galyean, M. L. Cole, N. A. Tedeschi, L. O. Branine, M. E. TI Efficiency of converting digestible energy to metabolizable energy and reevaluation of the California Net Energy System maintenance requirements and equations for predicting dietary net energy values for beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Review DE beef cattle; digestible energy; maintenance requirements; metabolizable energy; net energy ID WET DISTILLERS GRAINS; ENTERIC METHANE EMISSIONS; CARBON-NITROGEN BALANCE; GROWING CATTLE; NUTRIENT BALANCE; HEAT-PRODUCTION; FEED-INTAKE; CV BLANCA; STEERS; HEIFERS AB For the past several decades, nutrient requirement systems for beef cattle in North America have recommended that dietary ME can be calculated as dietary DE x 0.82, but considerable published data suggest a variable relationship between DE and ME. We reviewed the literature and tabulated the results of 23 respiration calorimetry studies (87 treatment mean data points), in which measurements of fecal, urinary, and gaseous energy were determined with beef cattle (bulls, steers, and heifers) and growing dairy cattle. Mixed-model regression analyses to adjust for the effects of the citation from which the data were obtained suggested a strong linear relationship between ME and DE (Mcal/kg of DM; ME = 0.9611 x DE - 0.2999; r(2) = 0.986, root mean square error [RMSE] = 0.048, P < 0.001 for intercept, slope. 0). Analysis of residuals from this simple linear regression equation indicated high correlations of residuals with other dietary components, and a slight increase in precision was obtained when dietary CP, ether extract, and starch (% of DM) concentrations were included in a multiple linear regression equation (citation-adjusted R-2 = 0.992, RMSE = 0.039). Using the simple linear relationship, we reevaluated the original data used to develop the California Net Energy System (CNES) for beef cattle by recalculating ME intake and heat production and regressing the logarithm of heat production on ME intake (both per BW, kg(0.75) daily). The resulting intercept and slope of the recalculated data did not differ (P >= 0.34) from those reported for the original analyses of the CNES data, suggesting that use of the linear equation for calculating ME concentration was consistent with NEm and NEg values as derived in the CNES. Nonetheless, because the cubic equations recommended by the NRC to calculate dietary NEm and NEg from ME were based on conversion of DE to ME using 0.82, these equations were mathematically recalculated to account for the linear relationship between DE and ME. Overall, our review and analyses suggested that there is a strong linear relationship between DE and ME, which seems to be consistent across a wide range of dietary conditions, cattle types, and levels of intake. Applying this linear relationship to predict ME concentrations agreed with the original CNES calculations for NE requirements, thereby allowing the development of new equations for predicting dietary NEm and NEg values from ME. C1 [Galyean, M. L.] Texas Tech Univ, Coll Agr Sci & Nat Resources, Lubbock, TX 79409 USA. [Cole, N. A.] USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. [Tedeschi, L. O.] Texas A&M Univ, College Stn, TX 77843 USA. [Branine, M. E.] Zinpro Corp, Canon City, CO 81212 USA. RP Galyean, ML (reprint author), Texas Tech Univ, Coll Agr Sci & Nat Resources, Lubbock, TX 79409 USA. EM michael.galyean@ttu.edu FU Jesse W. Thornton Chair in Animal Science Endowment; Paul Whitfield Horn Professorship funds at Texas Tech University FX Supported by the Jesse W. Thornton Chair in Animal Science Endowment and Paul Whitfield Horn Professorship funds at Texas Tech University. NR 44 TC 0 Z9 0 U1 5 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 IS 4 BP 1329 EP 1341 DI 10.2527/jas2015-0223 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RX UT WOS:000374692700001 PM 27135993 ER PT J AU Acharya, M Burke, JM Coffey, KP Kegley, EB Miller, JE Smyth, E Welborn, MG Terrill, TH Mosjidis, JA Rosenkrans, C AF Acharya, M. Burke, J. M. Coffey, K. P. Kegley, E. B. Miller, J. E. Smyth, E. Welborn, M. G. Terrill, T. H. Mosjidis, J. A. Rosenkrans, C., Jr. TI Changes in concentrations of trace minerals in lambs fed sericea lespedeza leaf meal pellets with or without dietary sodium molybdate SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE lambs; molybdenum; sericea lespedeza; trace minerals ID GASTROINTESTINAL NEMATODE INFECTION; CONDENSED TANNINS; HAEMONCHUS-CONTORTUS; LOTUS-PEDUNCULATUS; NUTRITIONAL-VALUE; GROWTH-RATE; GOATS; SHEEP; CUNEATA; HAY AB Prolonged feeding of sericea lespedeza (SL) previously led to reduced serum concentrations of Mo, a cofactor in an enzyme complex that may be involved in weight gain. The current objective was to determine the effect of Mo supplementation on changes in serum, fecal, urine, and liver concentrations of trace minerals in lambs fed SL leaf meal pellets. Thirty ram lambs weaned in May (84 +/- 1.5 d of age and 27 +/- 1.1 kg; D 0) were blocked by BW, breed type (full or three-fourths Katahdin), and EBV of parasite resistance and randomly assigned to be fed 900 g/d of an alfalfa-based supplement (CON; n = 10) or a SL-based supplement (n = 20) for 103 d. Supplements were formulated to be isonitrogenous and isocaloric and to meet trace mineral requirements. Within the SL group, individual lambs were administered either 5 mL water or 5 mL of water with 163.3 mg of sodium molybdate (SLMO). Serum was collected on d 28, 56, and 104; a liver sample was collected by biopsy on d 104 to determine concentrations of trace minerals. Data were analyzed using a mixed model and orthogonal contrasts. Serum concentrations of Mo increased in response to the drench and were greatest in SLMO lambs and then CON lambs and lowest in SL lambs (P < 0.001). Concentrations of Mo in the liver (P < 0.001) were similar between CON and SLMO lambs and were lower in SL lambs than other groups. Serum (P < 0.001) and liver (P = 0.013) concentrations of zinc (Zn) were reduced in both SL and SLMO lambs compared with CON lambs. Serum concentrations of cobalt (Co) increased in CON lambs compared with SL and SLMO lambs between d 0 and 56 but were similar on d 104 (diet x day, P < 0.005) as with concentrations in the liver. Serum and liver concentrations of copper (Cu) were greatest (P < 0.001 and P < 0.001, respectively) in CON lambs followed by SL lambs and then SLMO lambs. Serum concentrations of selenium (Se) tended (P = 0.10) to be reduced in SL lambs compared with CON and SLMO lambs, but concentrations in the liver were reduced in SL lambs compared with CON lambs and even more so in SLMO lambs (P < 0.003). Although the dietary Mo did increase stores of Mo in the animal and reduced copper, trace minerals associated with metalloproteins-Mo, copper, selenium, and zinc-were reduced in the liver of SL- and/or SLMO-fed lambs. These reductions could be associated with the lower weight gains previously observed after prolonged feeding of SL. C1 [Acharya, M.; Coffey, K. P.; Kegley, E. B.; Smyth, E.; Rosenkrans, C., Jr.] Univ Arkansas, Dept Anim Sci, Fayetteville, AR 72701 USA. [Burke, J. M.; Smyth, E.] ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Miller, J. E.; Welborn, M. G.] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA. [Terrill, T. H.] Ft Valley State Univ, Agr Res Stn, Ft Valley, GA 31030 USA. [Mosjidis, J. A.] Auburn Univ, Dept Crop Soil & Environm Sci, Auburn, AL 36849 USA. RP Burke, JM (reprint author), ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. EM joan.burke@ars.usda.gov FU USDA NIFA Organic Research and Education Initiative [2010-51300-21641]; USDA NIFA Small Business Innovative Research program [2011-33610-30836] FX Supported by USDA NIFA Organic Research and Education Initiative (project number 2010-51300-21641) and USDA NIFA Small Business Innovative Research program (project number 2011-33610-30836). NR 36 TC 0 Z9 0 U1 2 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2016 VL 94 IS 4 BP 1592 EP 1599 DI 10.2527/jas2015-9910 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RX UT WOS:000374692700026 PM 27136018 ER PT J AU Sisterson, MS Stenger, DC AF Sisterson, Mark S. Stenger, Drake C. TI Disentangling Effects of Vector Birth Rate, Mortality Rate, and Abundance on Spread of Plant Pathogens SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE epidemiology; vector-borne; pathogen; disease ID GLASSY-WINGED SHARPSHOOTER; EGG MATURATION STRATEGY; HOST-PLANT; VIRUS TRANSMISSION; DISEASE DYNAMICS; HOMALODISCA-VITRIPENNIS; BEMISIA-TABACI; LIFE-HISTORY; HEMIPTERA CICADELLIDAE; INSECTICIDE RESISTANCE AB Models on the spread of insect-transmitted plant pathogens often fix vector population size by assuming that deaths are offset by births. Although such mathematical simplifications are often justified, deemphasizing parameters that govern vector population size is problematic, as reproductive biology and mortality schedules of vectors of plant pathogens receive little empirical attention. Here, the importance of explicitly including parameters for vector birth and death rates was evaluated by comparing results from models with fixed vector population size with models with logistic vector population growth. In fixed vector population size models, increasing vector mortality decreased percentage of inoculative vectors, but had no effect on vector population size, as deaths were offset by births. In models with logistic vector population growth, increasing vector mortality decreased percentage of inoculative vectors and decreased vector population size. Consequently, vector mortality had a greater effect on pathogen spread in models with logistic vector population growth than in models with fixed vector population size. Further, in models with logistic vector population growth, magnitude of vector birth rate determined time required for vector populations to reach large size, thereby determining when pathogen spread occurred quickly. Assumptions regarding timing of vector mortality within a time step also affected model outcome. A greater emphasis of vector entomologists on studying reproductive biology and mortality schedules of insect species that transmit plant pathogens will facilitate identification of conditions associated with rapid growth of vector populations and could lead to development of novel control strategies. C1 [Sisterson, Mark S.; Stenger, Drake C.] USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. RP Sisterson, MS (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM mark.sisterson@ars.usda.gov; drake.stenger@ars.usda.gov FU USDA-ARS [2034-22000-010-00D] FX We thank Michael Jeger and Rodrigo Krugner for comments on an earlier draft of this manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. Funding for this research was provided by USDA-ARS appropriated project 2034-22000-010-00D. NR 73 TC 0 Z9 0 U1 7 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 487 EP 501 DI 10.1093/jee/tov329 PG 15 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000001 PM 26637536 ER PT J AU Corby-Harris, V Snyder, L Meador, CAD Naldo, R Mott, B Anderson, KE AF Corby-Harris, V. Snyder, L. Meador, C. A. D. Naldo, R. Mott, B. Anderson, K. E. TI Parasaccharibacter apium, gen. nov., sp nov., Improves Honey Bee ( Hymenoptera: Apidae) Resistance to Nosema SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Apis mellifera; honey bee; probiotic; Parasaccharibacter apium; Nosema ID WINTER COLONY LOSSES; UNITED-STATES; INFORMED PARTNERSHIP; LARVAL DEVELOPMENT; NATIONAL-SURVEY; MELLIFERA; CERANAE; INFECTION; GUT; MICROBIOME AB The honey bee, Apis mellifera L., is host to a variety of microorganisms. The bacterial community that occupies the adult worker gut contains a core group of approximately seven taxa, while the hive environment contains its own distribution of bacteria that is in many ways distinct from the gut. Parasaccharibacter apium, gen. nov., sp. nov., is a hive bacterium found in food stores and in larvae, worker jelly, worker hypopharyngeal glands, and queens. Parasaccharibacter apium increases larval survival under laboratory conditions. To determine if this benefit is extended to colonies in the field, we tested if P. apium 1) survives and reproduces in supplemental pollen patty, 2) is distributed throughout the hive when added to pollen patty, 3) benefits colony health, and 4) increases the ability of bees to resist Nosema. Parasaccharibacter apium survived in supplemental diet and was readily consumed by bees. It was distributed throughout the hive under field conditions, moving from the pollen patty to hive larvae. While P. apium did not significantly increase colony brood production, food stores, or foraging rates, it did increase resistance to Nosema infection. Our data suggest that P. apium may positively impact honey bee health. C1 [Corby-Harris, V.; Snyder, L.; Meador, C. A. D.; Naldo, R.; Mott, B.; Anderson, K. E.] USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA. [Corby-Harris, V.; Anderson, K. E.] Univ Arizona, Dept Entomol, Forbes 410,POB 210036, Tucson, AZ 85721 USA. RP Corby-Harris, V (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA.; Corby-Harris, V (reprint author), Univ Arizona, Dept Entomol, Forbes 410,POB 210036, Tucson, AZ 85721 USA. EM v.corbyharris@gmail.com; lucysnyder@email.arizona.edu; meadorcad@aol.com; rnaldo12@gmail.com; brendon.mott@ars.usda.gov; kirk.anderson@ars.us-da.gov FU United States Department of Agriculture (USDA) Agricultural Research Service (ARS); National Honey Board FX We acknowledge the staff of the Carl Hayden Bee Research Center and Gloria DeGrandi-Hoffman and Mark Carroll for comments on a previous version of the manuscript. This research was funded by the United States Department of Agriculture (USDA) Agricultural Research Service (ARS) and the National Honey Board. The ARS-USDA is an equal opportunity employer and provider. NR 44 TC 3 Z9 3 U1 4 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 537 EP 543 DI 10.1093/jee/tow012 PG 7 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000007 PM 26875068 ER PT J AU Hall, DG Albrecht, U Bowman, KD AF Hall, David G. Albrecht, Ute Bowman, Kim D. TI Transmission Rates of 'Ca. Liberibacter asiaticus' by Asian Citrus Psyllid Are Enhanced by the Presence and Developmental Stage of Citrus Flush SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diaphorina citri; huanglongbing; citrus greening ID DIAPHORINA-CITRI; HUANGLONGBING DISEASE; HEMIPTERA PSYLLIDAE; FEEDING-BEHAVIOR; INFECTION; SHOOTS; VECTOR; PLANTS; PCR AB Asian citrus psyllid (Diaphorina citri Kuwayama) transmits a bacterium 'Candidatus Liberibacter asiaticus' (CLas) putatively responsible for a devastating citrus disease known as Asiatic huanglongbing (HLB) (citrus greening disease). The psyllid and disease have invaded many citrus-growing regions including the United States, where the disease is seriously jeopardizing the Florida citrus industry. We recently concluded research that showed CLas transmission rates are increased when citrus flush is present. Flush is any new leaf growth ranging in development from first emergence up until the leaves are fully expanded yet still tender. In an experiment with seedlings of a rootstock cultivar 'US-942', a 1-wk infestation of 20 Asian citrus psyllids from an infected colony resulted in 53-60% of seedlings becoming infected when flush was present compared with only 7% when no flush was present. In a second experiment with 'US-942', 77-97% of seedlings became infected when flush was present compared with 40% when no flush was present. A similar experiment with 'Valencia' sweet orange resulted in 23, 80, and 3% seedlings becoming infected when young, older, or no flush was present, respectively. Young plants are therefore more likely to contract HLB if flush is present, with older flush promoting higher infection rates under the conditions of this study. Based on this finding, healthy citrus should be protected from Asian citrus psyllid infestations throughout a flush. To evaluate germplasm for CLas resistance, inoculations using infected Asian citrus psyllid would best be achieved if flush is present. C1 [Hall, David G.; Albrecht, Ute; Bowman, Kim D.] USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Albrecht, Ute] Univ Florida, Inst Food & Agr Sci, Southwest Florida Res & Educ Ctr, Dept Hort Sci, 2685 SR 29 N, Immokalee, FL 34142 USA. RP Hall, DG (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM David.Hall@ars.usda.gov; kim.bowman@ars.usda.gov FU Citrus Research and Development Foundation FX This research was supported in-part by grants from the Citrus Research and Development Foundation. We thank Kathy Moulton, Joanne Hodge, and Sailindra Patel (ARS-USDA, Fort Pierce, FL) for their contributions to this project. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. USDA is an equal opportunity provider and employer. NR 26 TC 4 Z9 5 U1 6 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 558 EP 563 DI 10.1093/jee/tow009 PG 6 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000009 PM 26884596 ER PT J AU Morales-Ramos, JA Rojas, MG Shelby, KS Coudron, TA AF Morales-Ramos, Juan A. Rojas, M. Guadalupe Shelby, Kent S. Coudron, Thomas A. TI Nutritional Value of Pupae Versus Larvae of Tenebrio molitor ( Coleoptera: Tenebrionidae) as Food for Rearing Podisus maculiventris ( Heteroptera: Pentatomidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Spined soldier bug; factitious prey; yellow mealworm; nutrition; predator rearing ID COLORADO POTATO BEETLE; PERILLUS-BIOCULATUS; SELF-SELECTION; MERIDIC DIETS; PREDATOR; PREY; REPRODUCTION; HEMIPTERA; CHRYSOMELIDAE; HYMENOPTERA AB Life-table analysis yielded demographic parameter values that indicate that Tenebrio molitor (L.) pupae are potentially more suitable factitious prey to mass-produce the predator Podisus maculiventris (Say) and are more suitable prey than the larvae. P. maculiventris developed faster (23.2 vs. 25.5 d), weighed more (females 80.9 vs. 66.6 mg and males 64.7 vs. 53.7 mg), and had a higher survival rate (0.88 vs. 0.7), fecundity, and reproductive output (87.1 vs. 22.8 eggs/female) when reared on pupae compared with larvae of T. molitor. The total protein content and soluble protein content were significantly higher in pupae (60.2 and 23%, respectively) than larvae (53.1 and 14.4%, respectively). Lipid content was significantly lower in pupae (32.1%) than larvae (35.9%), and larvae had more polyunsaturated fatty acids (83.6 vs. 56.6 mg/g) and less oleic (0.1 mg/g) and steric (6.1 mg/g) acids than pupae (37.3 and 12.3 mg/g, respectively). The total sugar content was not significantly different between pupae and larvae. However, larvae had significantly more fructose than pupae, but pupae had more galactose, glucosamine, glucose, mannose, and trehalose than larvae. Differences in nutritional composition and its impact on predator demographic parameters are potential factors that make the pupal stage a better food source. C1 [Morales-Ramos, Juan A.; Rojas, M. Guadalupe] USDA ARS, Natl Biol Control Lab, Stoneville, MS 38776 USA. [Shelby, Kent S.; Coudron, Thomas A.] USA Agr Res Serv, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Morales-Ramos, JA (reprint author), USDA ARS, Natl Biol Control Lab, Stoneville, MS 38776 USA. EM Juan.MoralesRamos@ARS.USDA.GOV; guadalupe.rojas@ars.usda.gov; kent.shelby@ars.usda.gov; tom.coudron@ars.usda.gov NR 44 TC 1 Z9 1 U1 5 U2 21 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 564 EP 571 DI 10.1093/jee/tov338 PG 8 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000010 PM 26628503 ER PT J AU Duarte, RT Goncalves, KC Espinosa, DJL Moreira, LF De Bortoli, SA Humber, RA Polanczyk, RA AF Duarte, R. T. Goncalves, K. C. Espinosa, D. J. L. Moreira, L. F. De Bortoli, S. A. Humber, R. A. Polanczyk, R. A. TI Potential of Entomopathogenic Fungi as Biological Control Agents of Diamondback Moth (Lepidoptera: Plutellidae) and Compatibility With Chemical Insecticides SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Plutella xylostella; biological control; chemical control; interaction ID XYLOSTELLA LINNAEUS LEPIDOPTERA; BEAUVERIA-BASSIANA; METARHIZIUM-ANISOPLIAE; BEMISIA-TABACI; LECANICILLIUM-MUSCARIUM; VERTICILLIUM-LECANII; PESTICIDES; RESISTANCE; CABBAGE; YPONOMEUTIDAE AB The objectives were to evaluate the efficiency of entomopathogenic fungi against Plutella xylostella ( L.) and the compatibility of the most virulent isolates with some of the insecticides registered for use on cabbage crops. Pathogenicity tests used isolates of Beauveria bassiana, Metarhizium rileyi, Isaria fumosorosea, Isaria sinclairii, and Lecanicillium muscarium standardized at a concentration of 10 7 conidia/ ml. Cabbage leaf discs were immersed in these suspensions, and after evaporation of the excess water, were placed 10 second- instar larvae of P. xylostella, totaling 10 leaf discs per treatment. Mortality was assessed 7 d after treatment, and the isolates that caused mortality > 80% were used to estimate LC50 and LT50. The compatibilities of the most virulent isolates and the insecticides were tested from the mixture of these into the culture medium, and after solidifying, the medium was inoculated with an aliquot of the isolated suspension. The following parameters were evaluated: growth of the colony, number and viability of conidia after 7 d. The isolated IBCB01, IBCB18, IBCB66, and IBCB87 of B. bassiana, LCMAP101 of M. rileyi, and ARSEF7973 of I. sinclairii caused mortality between 80 and 100%, with LC50 and LT50 between 2.504 to 6.775 10 4 conidia/ ml and 52.22 to 112.13 h, respectively. The active ingredients thiamethoxam and azadirachtin were compatible with the entomopathogenic fungi. The results suggest that the use of these isolates is an important alternative in the pesticidal management of P. xylostella, with the possible exception of the associated use of chemical controls using the active ingredients thiamethoxam or azadirachtin. C1 [Duarte, R. T.; Goncalves, K. C.; Espinosa, D. J. L.; Moreira, L. F.; De Bortoli, S. A.; Polanczyk, R. A.] Sao Paulo State Univ Julio de Mesquita Filho Unes, Fac Agron & Vet Sci, Dept Plant Protect, BR-14884900 Jaboticabal, SP, Brazil. [Humber, R. A.] USDA ARS, Emerging Pests & Pathogens Res, Robert W Holly Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Duarte, RT (reprint author), Sao Paulo State Univ Julio de Mesquita Filho Unes, Fac Agron & Vet Sci, Dept Plant Protect, BR-14884900 Jaboticabal, SP, Brazil. EM rogerio.tduarte@yahoo.com.br; kellcrist2010@hotmail.com; daespi24.7@gmail.com; laka_moreira@hotmail.com; bortoli@fcav.unesp.br; richard.humber@ars.usda.gov; rapolanc@yahoo.com.br NR 72 TC 1 Z9 1 U1 12 U2 21 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 594 EP 601 DI 10.1093/jee/tow008 PG 8 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000014 PM 26850733 ER PT J AU Vargas, RI Souder, SK Morse, JG Grafton-Cardwell, EE Haviland, DR Kabashima, JN Faber, BA Mackey, B Cook, P AF Vargas, Roger I. Souder, Steven K. Morse, Joseph G. Grafton-Cardwell, Elizabeth E. Haviland, David R. Kabashima, John N. Faber, Ben A. Mackey, Bruce Cook, Peter TI Captures of Wild Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae ( Diptera: Tephritidae) in Traps with Improved Multilure TMR Dispensers Weathered in California SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tephritidae; fruit fly; detection; survey; eradication program ID METHYL EUGENOL; MALE LURES; HAWAII; FIELD AB During 2012- 2013, solid Mallet TMR ( trimedlure [ TML], methyl eugenol [ ME], raspberry ketone [ RK]) wafers impregnated with DDVP ( 2, 2- dichlorovinyl dimethyl phosphate) insecticide were weathered during summer ( 8 wk) and winter ( 12 wk) in five California citrus- growing counties ( Kern, Ventura, Orange, Tulare, and Riverside). In addition, TMR wafers without DDVP and with a Hercon Vaportape II insecticidal strip were compared with TMR dispensers with DDVP at Exeter and Riverside. Weathered treatments were shipped every week ( overnight delivery) to Hawaii and frozen for a later bioassay in a 1,335- ha coffee plantation near Numila, Kauai Island, HI, where Mediterranean fruit fly, Ceratitis capitata ( Wiedemann), oriental fruit fly, Bactrocera dorsalis Hendel, and melon fly, Bactrocera cucurbitae Coquillett, were all present. We compared trap captures of the three species, C. capitata, B. dorsalis, and B. cucurbitae, for the five different weathering locations. Captures of C. capitata, B. dorsalis, and B. cucurbitae with Mallet TMR dispensers ( with DDVP) were not significantly different for the five locations. Captures with the Mallet TMR dispenser without DDVP and Vaportape were similar to those for Mallet TMR with DDVP, although there were some slight location differences. In conclusion, based on these results, the Mallet TMR dispenser could potentially be used in California habitats where large numbers of detection traps are currently deployed. Use of Vaportape with dispensers would not require them to be registered with US Environmental Protection Agency ( EPA). Dispensers for use as Male Annihilation Technique ( MAT) devices will be tested further in Hawaii. C1 [Vargas, Roger I.; Souder, Steven K.] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Morse, Joseph G.; Grafton-Cardwell, Elizabeth E.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Haviland, David R.] Univ Calif Cooperat Extens Kern Co, Bakersfield, CA 93307 USA. [Kabashima, John N.] Univ Calif Cooperat Extens Orange Co, Irvine, CA 92618 USA. [Faber, Ben A.] Univ Calif Cooperat Extens Ventura Co, Ventura, CA 93003 USA. [Mackey, Bruce] USDA ARS PWA, Albany, CA 94710 USA. [Cook, Peter] Farma Tech Int, North Bend, WA 98045 USA. RP Vargas, RI (reprint author), USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM roger.vargas@ars.usda.gov; steven.souder@ars.usda.gov; joseph.morse@ucr.edu; eegraftoncardwell@ucanr.edu; dhaviland@ucdavis.edu; jnkabashima@ucanr.edu; bafaber@ucanr.edu; bruce.mackey@ars.usda.gov; pjcook@farmatech.com FU California Citrus Research Board [5500-193]; [IR-4 (2013-34383-20981)] FX We thank Russell Ijima, Charmaine D. Sylva, Yu Nakane, Dominique Easter-Green, Jacqueline De La Garza, Daisy L. Willis, and Jordan "Kama" Lee Loy (Daniel K. Inouye Pacific Basin Agricultural Research Center, USDA-ARS, Hilo, HI) for data collection and logistical support in Hawaii. We also thank Lindsay J. Robinson, Monica J. Dimson, Stephanie M. Rill, Anita M. Hunt, Sara Scott, Jamie Nemecek, Jennifer Ruvalcaba, Therese Kapaun, Leah Haynes, Beryl Feller, Lee Rosenboom, Rebecca Montgomery, and Marian Fleming for logistical and experimental support in California. We thank the University of California Agriculture and Natural Resources Lindcove Research and Extension Center, UC ANR Lindcove Research and Extension Center, and Hansen Research and Extension Center in California for providing access to citrus groves for trap weather. We also thank A&B Properties, Kauai Coffee Co., and in particular, Greg Williams, for allowing us to use sections of their coffee plantation for evaluations. We thank Nicholas Manoukis and John Stark for comments on an earlier drafts of this manuscript. Finally, we thank the California Citrus Research Board (5500-193) and IR-4 (2013-34383-20981) for partial financial support for this work. NR 16 TC 0 Z9 0 U1 5 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 607 EP 612 DI 10.1093/jee/tov327 PG 6 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000016 PM 26582906 ER PT J AU Mankin, RW Al-Ayedh, HY Aldryhim, Y Rohde, B AF Mankin, R. W. Al-Ayedh, H. Y. Aldryhim, Y. Rohde, B. TI Acoustic Detection of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) and Oryctes elegans (Coleoptera: Scarabaeidae) in Phoenix dactylifera (Arecales: Arecacae) Trees and Offshoots in Saudi Arabian Orchards SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE red palm weevil; date palm fruit stalk borer; scouting; pest management ID RED PALM WEEVIL; DATE PALMS; CURCULIONIDAE; MANAGEMENT; LARVAE; INFESTATION; PERSPECTIVE; PATTERNS; ECOLOGY; SYSTEMS AB Rhynchophorus ferrugineus ( Olivier) ( Coleoptera: Dryophthoridae) larvae are cryptic, internal tissue- feeding pests of palm trees that are difficult to detect; consequently, infestations may remain hidden until they are widespread in an orchard. Infested trees and propagable offshoots that develop from axillary buds on the trunk frequently are transported inadvertently to previously uninfested areas. Acoustic methods can be used for scouting and early detection of R. ferrugineus, but until now have not been tested on multiple trees and offshoots in commercial date palm orchard environments. For this report, the acoustic detectability of R. ferrugineus was assessed in Saudi Arabian date palm orchards in the presence of commonly occurring wind, bird noise, machinery noise, and nontarget insects. Signal analyses were developed to detect R. ferrugineus and another insect pest, Oryctes elegans Prell ( Coleoptera: Scarabaeidae), frequently co- occurring in the orchards, and discriminate both from background noise. In addition, it was possible to distinguish R. ferrugineus from O. elegans in offshoots by differences in the temporal patterns of their sound impulses. As has been observed often with other insect pests, populations of the two species appeared clumped rather than uniform or random. The results are discussed in relation to development of automated methods that could assist orchard managers in quickly identifying infested trees and offshoots so that R. ferrugineus infestations can be targeted and the likelihood of transferring infested offshoots to uninfested areas can be reduced. C1 [Mankin, R. W.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Al-Ayedh, H. Y.] King Abdulaziz City Sci & Technol, Life Sci & Environm Res Inst, Riyadh 11442, Saudi Arabia. [Aldryhim, Y.] King Saud Univ, Dept Plant Protect, Coll Food & Agr Sci, Econ Entomol Res Unit, Riyadh 11451, Saudi Arabia. [Rohde, B.] Univ Florida, Dept Elect & Comp Engn, Gainesville, FL 32611 USA. RP Mankin, RW (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM Richard.Mankin@ars.usda.gov; alayedh@kacst.edu.sa; aldryhim@hotmail.com; barukh94-work@yahoo.com FU King Abdulaziz City for Science and Technology, Saudi Arabia [597-32]; National Science Foundation [DGE-1315138] FX We appreciate the scouting and technical assistance of Mousa Alolaywi, Dahi Abdulkader, Adam Hamed, Saad AlQahtani, Abdulrman Alsherhri, and Ahmad Almutairi. This research was supported by the King Abdulaziz City for Science and Technology, Saudi Arabia, Project number 597-32, and National Science Foundation Graduate Research Fellowship DGE-1315138. NR 34 TC 0 Z9 0 U1 3 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 622 EP 628 DI 10.1093/jee/tov398 PG 7 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000018 PM 26743218 ER PT J AU Spurgeon, DW AF Spurgeon, Dale W. TI Captures of Boll Weevils (Coleoptera: Curculionidae) in Relation to Trap Orientation and Distance From Brush Lines SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE boll weevil; Anthonomus grandis; pheromone; trapping ID PHEROMONE TRAPS; DISPERSAL AB Eradication programs for the boll weevil (Anthonomus grandis grandis Boheman) rely on pheromone-baited traps to trigger insecticide treatments and monitor program progress. A key objective of monitoring in these programs is the timely detection of incipient weevil populations to limit or prevent re-infestation. Therefore, improvements in the effectiveness of trapping would enhance efforts to achieve and maintain eradication. Association of pheromone traps with woodlots and other prominent vegetation are reported to increase captures of weevils, but the spatial scale over which this effect occurs is unknown. The influences of trap distance (0, 10, and 20 m) and orientation (leeward or windward) to brush lines on boll weevil captures were examined during three noncropping seasons (October to February) in the Rio Grande Valley of Texas. Differences in numbers of captured weevils and in the probability of capture between traps at 10 or 20 m from brush, although often statistically significant, were generally small and variable. Variations in boll weevil population levels, wind directions, and wind speeds apparently contributed to this variability. In contrast, traps closely associated with brush (0 m) generally captured larger numbers of weevils, and offered a higher probability of weevil capture compared with traps away from brush. These increases in the probability of weevil capture were as high as 30%. Such increases in the ability of traps to detect low-level boll weevil populations indicate trap placement with respect to prominent vegetation is an important consideration in maximizing the effectiveness of trap-based monitoring for the boll weevil. C1 [Spurgeon, Dale W.] ARS, USDA, Arid Land Agr Res Ctr, 21881N Cardon Lane, Maricopa, AZ 85138 USA. RP Spurgeon, DW (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881N Cardon Lane, Maricopa, AZ 85138 USA. EM Dale.Spurgeon@ars.usda.gov NR 21 TC 1 Z9 1 U1 1 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 676 EP 683 DI 10.1093/jee/tov392 PG 8 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000025 PM 26719592 ER PT J AU Mahmoud, MAB Sharp, RE Oliver, MJ Finke, DL Ellersieck, MR Hibbard, BE AF Mahmoud, M. A. B. Sharp, R. E. Oliver, M. J. Finke, D. L. Ellersieck, M. R. Hibbard, B. E. TI The Effect of Western Corn Rootworm (Coleoptera: Chrysomelidae) and Water Deficit on Maize Performance Under Controlled Conditions SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; drought; maize; damage ID GAS-EXCHANGE PARAMETERS; OSMOTIC ADJUSTMENT; LARVAL INJURY; SOIL-MOISTURE; SIGNAL-TRANSDUCTION; PLANT-RESPONSES; TRANSGENIC CORN; DRYING SOIL; GROWTH; STRESS AB The western corn rootworm, Diabrotica virgifera virgifera LeConte, is the most important insect of maize, Zea mays L., but knowledge of its interaction with water deficit on maize production is lacking. A series of greenhouse experiments using three infestation levels of the western corn rootworm, D. virgifera virgifera, under well-watered, moderately dry, and very dry soil moisture levels were conducted to quantify the interaction of western corn rootworm and soil water deficit on B73 x Mo17 maize growth and physiology. Three separate experiments were conducted. Soil moisture regimes were initiated 30 d postplanting for experiments using neonate and second-instar larvae and 30 d postinfestation in the experiment using eggs. In the neonate and second-instar experiments, there were no significant differences among western corn rootworm levels in their effects on leaf water potential, shoot dry weight, and root dry weight. The interaction of western corn rootworm and soil moisture significantly impacted the larval recovery in the neonate experiment, but no other significant interactions were documented between soil moisture levels and rootworm infestation levels. Overall, the results indicate that under the conditions of these experiments, the effect of water deficit was much greater on plants than the effect of western corn rootworm and that the interactions between water deficit and western corn rootworm levels minimally affected the measured parameters of plant performance. C1 [Mahmoud, M. A. B.; Sharp, R. E.; Oliver, M. J.; Hibbard, B. E.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Mahmoud, M. A. B.; Sharp, R. E.; Oliver, M. J.; Finke, D. L.; Hibbard, B. E.] Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO 65211 USA. [Mahmoud, M. A. B.] South Valley Univ, Dept Zool, Fac Sci, Qena 83523, Egypt. [Oliver, M. J.; Hibbard, B. E.] USDA ARS, Plant Genet Res Unit, 205 Curtis Hall, Columbia, MO 65211 USA. [Ellersieck, M. R.] Univ Missouri, Columbia, MO 65211 USA. RP Hibbard, BE (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.; Hibbard, BE (reprint author), Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO 65211 USA.; Hibbard, BE (reprint author), USDA ARS, Plant Genet Res Unit, 205 Curtis Hall, Columbia, MO 65211 USA. EM mampr7@mail.missouri.edu; SharpR@missouri.edu; Mel.Oliver@ars.usda.gov; finked@missouri.edu; EllersieckM@missouri.edu; Bruce.Hibbard@ars.usda.gov FU Egyptian government; USDA-ARS; University of Missouri, Division of Plant Sciences FX We thank Dalton Ludwick (University of Missouri) for assistance with final tables and figures. We thank Sherry Flint-Garcia (USDA-ARS, Columbia, MO) for seed. We thank Sarah Zukoff (now with Kansas State University) and Lisa Meihls (USDA-ARS, Columbia, MO) for their assistance in editing an earlier draft. We thank Julie Barry and Jim Elder (USDA-ARS, Columbia, MO), for assistance in setting up the experiment and calibrating equipment. We thank Tim Wooldridge (University of Missouri) for assistance with data collection. We also thank the four anonymous reviewers who provided comments to improve this manuscript. Finally, we thank the Egyptian government, USDA-ARS, and the University of Missouri, Division of Plant Sciences for financial assistance. NR 75 TC 0 Z9 0 U1 6 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 684 EP 698 DI 10.1093/jee/tow011 PG 15 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000026 PM 26896532 ER PT J AU Duan, JJ Aparicio, E Tatman, D Smith, MT Luster, DG AF Duan, Jian J. Aparicio, Ellen Tatman, Daria Smith, Michael T. Luster, Doug G. TI Potential New Associations of North American Parasitoids With the Invasive Asian Longhorned Beetle (Coleoptera: Cerambycidae) for Biological Control SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Anoplophora glabripennis; wood borer; Braconidae; parasitism; Ontsira mellipes ID ANOPLOPHORA-GLABRIPENNIS COLEOPTERA; CONTROL AGENTS; HOST; HYMENOPTERA; EXPERIENCE AB The Asian longhorned beetle, Anoplophora glabripennis ( Motschulsky), is a polyphagous wood- boring insect native to Asia. Since it invaded North America in the 1990s, the beetle has been continuously targeted by quarantines and eradication programs in the United States and Canada. We examined the potential for development of new species- associations between A. glabripennis and hymenopteran parasitoids collected from cerambycids and other wood- boring insects infesting red maple ( Acer rubrum L.) trees in the midAtlantic region of the United States. Results of our study showed that five groups of braconid parasitoids ( Ontsira mellipes Ashmead, Rhoptrocentrus piceus Marsh, Spathius laflammei Provancher, Heterospilus spp., and Atanycolus spp.) successfully attacked early instars of A. glabripennis larvae infesting red maple logs and produced both male and female progenies. One species, O. mellipes, was continuously reared on A. glabripennis larvae inserted inside small red maple sticks for over 50 generations, and produced femalebiased progeny ( 6: 1 female to male ratio) at each generation. Continuous rearing of O. mellipes on A. glabripennis larvae did not significantly increase the parasitism and mean number of progeny produced per parasitized host. Together, these findings demonstrate that some North American parasitoids may be able to develop new associations with A. glabripennis and thus should be further studied under semifield or field conditions for possible use in biocontrol. C1 [Duan, Jian J.; Aparicio, Ellen; Tatman, Daria; Smith, Michael T.; Luster, Doug G.] USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. RP Duan, JJ (reprint author), USDA ARS, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov; Ellen.Aparicio@ars.usda.gov; Daria.Tatman@ars.usda.gov; lucgus2754@gmail.com; doug.luster@ars.usda.gov NR 32 TC 3 Z9 3 U1 17 U2 20 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 699 EP 704 DI 10.1093/jee/tov328 PG 6 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000027 PM 26602779 ER PT J AU Poland, TM Ciaramitaro, TM McCullough, DG AF Poland, Therese M. Ciaramitaro, Tina M. McCullough, Deborah G. TI Laboratory Evaluation of the Toxicity of Systemic Insecticides to Emerald Ash Borer Larvae SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE emerald ash borer; larval mortality; systemic insecticide; toxicity; lethal concentration ID PLANIPENNIS COLEOPTERA BUPRESTIDAE; ANOPLOPHORA-GLABRIPENNIS COLEOPTERA; NORTH-AMERICA; URBAN FOREST; NEONICOTINOID INSECTICIDES; TEMPORAL DISTRIBUTION; MANAGEMENT OPTIONS; EMAMECTIN BENZOATE; CERAMBYCIDAE; INVASION AB Emerald ash borer (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae), an invasive phloem-feeding insect native to Asia, threatens at least 16 North American ash (Fraxinus) species and has killed hundreds of millions of ash trees in landscapes and forests. We conducted laboratory bioassays to assess the relative efficacy of systemic insecticides to control emerald ash borer larvae in winter 2009 and 2010. Second- and third-instar larvae were reared on artificial diet treated with varying doses of emamectin benzoate (TREE-age, Arborjet, Inc., Woburn, MA), imidacloprid (Imicide, J. J Mauget Co., Arcadia, CA), dinotefuran (Safari, Valent Professional Products, Walnut Creek, CA), and azadirachtin (TreeAzin, BioForest Technologies, Inc., Sault Ste. Marie, Ontario, and Azasol, Arborjet, Inc., Woburn, MA). All of the insecticides were toxic to emerald ash borer larvae, but lethal concentrations needed to kill 50% of the larvae (LC50), standardized by larval weight, varied with insecticide and time. On the earliest date with a significant fit of the probit model, LC50 values were 0.024 ppm/g at day 29 for TREE-age, 0.015 ppm/g at day 63 for Imicide, 0.030 ppm/g at day 46 for Safari, 0.025 ppm/g at day 24 for TreeAzin, and 0.027 ppm/g at day 27 for Azasol. The median lethal time to kill 50% (LT50) of the tested larvae also varied with insecticide product and dose, and was longer for Imicide and Safari than for TREE-age or the azadirachtin products. Insecticide efficacy in the field will depend on adult and larval mortality as well as leaf and phloem insecticide residues. C1 [Poland, Therese M.; Ciaramitaro, Tina M.] US Forest Serv, USDA, No Res Stn, 3101 Technol Blvd,Suite F, Lansing, MI 48910 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. RP Poland, TM (reprint author), US Forest Serv, USDA, No Res Stn, 3101 Technol Blvd,Suite F, Lansing, MI 48910 USA. EM tpoland@fs.fed.us; tciaramitaro@fs.fed.us; mccullo6@msu.edu FU USDA Forest Service Pesticide Impact Assessment Program FX We thank Debbie Miller and Monica Hufnagel for assistance with emerald ash borer rearing and bioassays, John Stanovick for statistical advice, and Melody Keena and David Mota-Sanchez for reviewing an earlier draft of the manuscript. This research was supported by a grant from the USDA Forest Service Pesticide Impact Assessment Program. NR 54 TC 0 Z9 0 U1 5 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 705 EP 716 DI 10.1093/jee/tov381 PG 12 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000028 PM 26721288 ER PT J AU Nino-Dominguez, A Sullivan, BT Lopez-Urbina, JH Macias-Samano, JE AF Nino-Dominguez, Alicia Sullivan, Brian T. Lopez-Urbina, Jose H. Macias-Samano, Jorge E. TI Responses by Dendroctonus frontalis and Dendroctonus mesoamericanus ( Coleoptera: Curculionidae) to Semiochemical Lures in Chiapas, Mexico: Possible Roles of Pheromones During Joint Host Attacks SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE coexistence; bark beetle; reproductive isolation; syntopic species; pheromone ID SOUTHERN PINE-BEETLE; BARK BEETLES; ATTRACTANT PHEROMONE; ENDO-BREVICOMIN; UNITED-STATES; SCOLYTIDAE; IPS; AGGREGATION; ENANTIOMERS; BEHAVIOR AB In southern Mexico and Central America, the southern pine beetle Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae) commonly colonizes host trees simultaneously with Dendroctonus mesoamericanus Armendariz-Toledano and Sullivan, a recently described sibling species. We hypothesized that cross-species pheromone responses by host-seeking beetles might mediate joint mass attack, bole partitioning, and reproductive isolation between the species. Previous studies had indicated that D. frontalis females produce frontalin and that female D. mesoamericanus produce frontalin, endo-brevicomin, and ipsdienol (males of both species produce endo-brevicomin and possibly ipsdienol). In field trapping trials in the Mexican state of Chiapas, D. frontalis was attracted to the lure combination of turpentine and racemic frontalin; racemic endo-brevicomin enhanced this response. In a single test, D. mesoamericanus was attracted in low numbers to the combination of turpentine, racemic frontalin, and racemic endo-brevicomin after the addition of racemic ipsdienol; in contrast, racemic ipsdienol reduced responses of D. frontalis. Inhibition of D. frontalis was generated in both sexes by (+)- and racemic ipsdienol, but by (-)-ipsdienol only in females. Logs infested with D. mesoamericanus females (the pioneer sex in Dendroctonus) attracted both species in greater numbers than either D. frontalis female-infested or uninfested logs. Our data imply that D. frontalis may be more attracted to pioneer attacks of D. mesoamericanus females, and that this could be owing to the presence of endo-brevicomin in the latter. Possible intra- and inter-specific functions of semiochemicals investigated in our experiments are discussed. C1 [Nino-Dominguez, Alicia; Lopez-Urbina, Jose H.; Macias-Samano, Jorge E.] Colegio Frontera ECOSUR, Carretera Antiguo Aeropuerto Km 2-5, Tapachula 30700, Chiapas, Mexico. [Sullivan, Brian T.] US Forest Serv, USDA, Southern Res Stn, 2500 Shreveport Highway, Pineville, LA 71360 USA. RP Sullivan, BT (reprint author), US Forest Serv, USDA, Southern Res Stn, 2500 Shreveport Highway, Pineville, LA 71360 USA. EM pupa_rlaveu@hotmail.com; briansullivan@fs.fed.us; urbina_jose@hotmail.com; jemaciass58@gmail.com FU Consejo Nacional de Ciencia y Tecnologia (CONACYT) [316670]; USDA Forest Service [11-IC-11330129-046] FX We wish to thank Gerardo Zuniga, Pablo Liedo Fernandez, and Leopoldo Cruz Lopez for their academic support and consultations on the research; also Don Roberto Castellanos for his very enthusiastic field support. Thanks to Rosalba Reyes, Hector Montano Moreno, and Aldo A. De la Mora for their help in trap-catch collections, and to Javier Valle Mora for his valuable comments on statistical analysis. We want to thank the Comision Nacional de Areas Naturales Protegidas (CONANP) for cooperation and use of facilities during work in Parque Nacional Lagunas de Montebello; also the Consejo Nacional de Ciencia y Tecnologia (CONACYT) for the lead author's doctoral fellowship (grant number 316670). William P. Shepherd provided comments on a draft of this paper. Research was supported in part by USDA Forest Service Cooperative Agreement 11-IC-11330129-046. NR 39 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 724 EP 731 DI 10.1093/jee/tov406 PG 8 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000030 PM 26803816 ER PT J AU Osbrink, WLA Cornelius, ML Showler, AT AF Osbrink, W. L. A. Cornelius, M. L. Showler, A. T. TI Bionomics and Formation of "Bonsai" Colonies With Long-Term Rearing of Coptotermes formosanus (Isoptera: Rhinotermitidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Formosan subterranean termite; colony effect ID SUBTERRANEAN TERMITE; SHIRAKI ISOPTERA; NEW-ORLEANS; INDEPENDENT MONITORS; URBAN-ENVIRONMENT; FIELD POPULATIONS; LOUISIANA; AREA; BIOLOGY; PROPORTIONS AB This laboratory study reports the ability of Formosan subterranean termite, Coptotermes formosanus Shiraki, colonies to survive for at least 9 yr while restricted to a sweater box. Colonies survived by limiting queen size and worker numbers, allowing these bonsai colonies to thrive. Queen physogastry appeared to plateau with 9-yr-old queens not larger than 6-yr-old queens, but nearly triple the size of 2-yr-old queens. Nine-year-old colony worker numbers were not greater than 6-yr-old colonies, but worker numbers were greater than in 2-yr-old colonies. Such colony survival under conditions of restricted resources provides a mechanism for re-infestation of areas following extensive area-wide control efforts. "Bonsai" colonies are relevant to the ability of marginalized colonies to avoid detection and then expand and invade into areas once the large, mature colonies are eliminated, and their potential to produce alates to start new C. formosanus colonies in areas which have been subjected to colony elimination programs impacts termite management strategies. C1 [Osbrink, W. L. A.; Showler, A. T.] USDA ARS SPA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Cornelius, M. L.] USDA ARS BARC, 10300 Baltimore Ave,Bldg 007,Rm 313, Beltsville, MD 20705 USA. RP Osbrink, WLA (reprint author), USDA ARS SPA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM weste.osbrink@ars.usda.gov; mary.cornelius@ars.usda.gov; allan.showler@ars.usda.gov NR 47 TC 0 Z9 0 U1 1 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 770 EP 778 DI 10.1093/jee/tov346 PG 9 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000036 PM 26662736 ER PT J AU Geisert, RW Ellersieck, MR Hibbard, BE AF Geisert, Ryan W. Ellersieck, Mark R. Hibbard, Bruce E. TI Tolerance of eCry3.1Ab in Reciprocal Cross Offspring of eCry3.1Ab-Selected and Control Colonies of Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bt resistance; Bacillus thuringiensis; insect resistance management ID WESTERN CORN-ROOTWORM; FIELD-EVOLVED RESISTANCE; TRANSGENIC-MAIZE; INSECT RESISTANCE; BT MAIZE; MORTALITY; LARVAE; PEST; SUSTAINABILITY; SUSCEPTIBILITY AB Two new insect colonies were created by separating virgin western corn rootworm, Diabrotica virgifera virgifera LeConte, males and females from both a selected laboratory colony that was being reared on eCry3.1Ab-expressing corn (Zea mays L.) and a control colony reared on its near-isoline corn. Females from the selected colony were paired with males of the control colony and vice versa to create both a selected female by control male colony (Sel female) and control female by selected male colony (Con female). Both colonies along with their parental colonies (eCry3.1Ab-selected and control) were evaluated on eCry3.1Ab-expressing corn and its near-isoline in seedling assays. Larvae from each colony were also used in diet toxicity experiments in order to determine the LC50 and EC50 values for the eCry3.1Ab toxin for each. Statistical analysis of seedling assay experiments did not indicate any significant colony x corn interaction but did show a significant main effect of corn type for both larval recovery and larval head capsule widths. Results from the diet toxicity assays showed the control colony to have a significantly lower LC50 value than the selected and cross colonies and a significantly lower EC50 than the selected and Con female colonies. Calculations of dominance values (h) of eCry3.1Ab resistance traits from seedling assays indicated that the two reciprocal cross colonies have a dominance value (h) of similar to 1, suggesting dominance of the eCry3.1Ab resistance trait. C1 [Geisert, Ryan W.] Univ Missouri, Div Plant Sci, 205 Curtis Hall, Columbia, MO 65211 USA. [Ellersieck, Mark R.] Univ Missouri, Agr Expt Stn Statistician, Columbia, MO 65211 USA. [Hibbard, Bruce E.] Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. RP Hibbard, BE (reprint author), Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. EM rwg5h8@mail.missouri.edu; EllersieckM@missouri.edu; Bruce.Hibbard@ars.usda.gov FU Syngenta FX We thank Julie Barry (USDA-ARS) for technical assistance in rearing parental colonies and suggestions with the reciprocal cross colonies. We thank Bruce Lang (Custom Bio Products) for the processing of diet toxicity bioassays. We thank Syngenta for providing funding, Bt and isoline seed, as well as protein for toxicity bioassays. NR 45 TC 0 Z9 0 U1 3 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 815 EP 820 DI 10.1093/jee/tov335 PG 6 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000042 PM 26628501 ER PT J AU Chaudhury, MF Zhu, JJ Skoda, SR AF Chaudhury, M. F. Zhu, J. J. Skoda, S. R. TI Bacterial Volatiles Attract Gravid Secondary Screwworms (Diptera: Calliphoridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cochliomyia macellaria; attractant; bloodmeal; oviposition ID COCHLIOMYIA-HOMINIVORAX DIPTERA; INCUBATED BOVINE BLOOD; MEDIATE OVIPOSITION; MACELLARIA DIPTERA; FLIES DIPTERA; RESPONSES; FLY; IDENTIFICATION; BEHAVIOR; WOUNDS AB Bovine blood inoculated and incubated with bacteria was tested to determine if secondary screwworm, Cochliomyia macellaria (F.), would be attracted to the incubated substrate for oviposition. Five species of bacteria, Klebsiella oxytoca (Flugge), Proteus mirabilis Hauser, Proteus vulgaris Hauser, Providencia rettgeri Hadley, Elkins and Caldwell, and Providencia stuartii Ewing, previously isolated from animal wounds infested by primary screwworms, Cochliomyia hominivorax (Coquerel), were used. Incubated substrates were tested in a two-choice cage bioassay to study landing response and oviposition by gravid C. macellaria. Significantly more flies landed on substrates containing P. mirabilis than on substrates with other species of bacteria. Klebsiella oxytoca-treated substrates attracted the least flies. Substrates containing bacteria incubated for 72 h attracted significantly more flies than those incubated for 24-, 48-, or 96-h period. In 3-h duration oviposition tests, substrates with P. rettgeri attracted significantly more flies to oviposit than the other four species. The most eggs were recorded when substrates treated with all five species of bacteria were offered for oviposition. It is likely that multiple active chemicals present in the volatiles from substrates treated with all five species result in greater response than those in a single species. At least 72-h incubation seems to be necessary to obtain the most active volatile chemicals. Results suggest that C. macellaria uses similar chemical cues as C. hominivorax from bacteria volatiles as oviposition attractant/stimulant. C1 [Chaudhury, M. F.] Univ Nebraska, USDA ARS, Screwworm Res Unit, UNL EC, 109 Entomol Hall, Lincoln, NE 68583 USA. [Zhu, J. J.] Univ Nebraska, USDA ARS, Agroecosyst Management Res Unit, UNL EC, 305 Entomol Hall, Lincoln, NE 68583 USA. [Skoda, S. R.] USDA ARS, Screwworm Res Unit, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. RP Chaudhury, MF (reprint author), Univ Nebraska, USDA ARS, Screwworm Res Unit, UNL EC, 109 Entomol Hall, Lincoln, NE 68583 USA. EM Muhammad.Chaudhury@ars.usda.gov; Jerry.Zhu@ars.usda.gov; steve.skoda@ars.usda.gov NR 28 TC 1 Z9 1 U1 1 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 947 EP 951 DI 10.1093/jee/tov390 PG 5 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000059 PM 26748982 ER PT J AU Burks, CS Kuenen, LPS Daane, KM AF Burks, Charles S. Kuenen, L. P. S. 'Bas' Daane, Kent M. TI Phenyl Propionate and Sex Pheromone for Monitoring Navel Orangeworm (Lepidoptera: Pyralidae) in the Presence of Mating Disruption SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Amyelois transitella; monitoring; attractant; pheromone ID CODLING MOTH LEPIDOPTERA; AMYELOIS-TRANSITELLA; ECTOMYELOIS-CERATONIAE; ETHYL (E; IDENTIFICATION; Z)-2,4-DECADIENOATE; ATTRACTANTS; TORTRICIDAE; VOLATILES; ALMOND AB The recent availability of sex pheromone lures for the navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), improves options for monitoring this key pest in conventionally managed almonds. These lures are, however, minimally effective in the presence of mating disruption. Experiments were conducted to determine if phenyl propionate (PPO), an attractant for the navel orangeworm, acts in an additive or synergistic manner when presented together with the pheromone. In the absence of mating disruption, traps baited with PPO captured significantly fewer adults than traps baited with a sex pheromone lure. There was no difference in the number of adults captured in traps with both attractants when mating disruption was not used. In the presence of mating disruption, pheromone traps were completely suppressed, yet traps with both pheromone and PPO captured significantly more adults than traps baited with only PPO. Traps with only PPO captured equal numbers of both sexes, whereas traps with both attractants had significantly more males. These findings demonstrate that PPO is likely to be useful for monitoring navel orangeworm in fields treated with mating disruption. C1 [Burks, Charles S.; Kuenen, L. P. S. 'Bas'] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Burks, CS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM charles.burks@ars.usda.gov; bearman55@gmail.com; kdaane@ucanr.edu FU Specialty Crops Block Grant from the California Department of Food and Agriculture FX We thank Mario Salinas, Matt Hicks, and Lino Salinas for technical assistance. This study was partially funded by a Specialty Crops Block Grant from the California Department of Food and Agriculture. NR 27 TC 0 Z9 0 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 958 EP 961 DI 10.1093/jee/tov339 PG 4 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000061 PM 26628502 ER PT J AU Nguyen, P Kim, AY Jung, JK Donahue, KM Jung, C Choi, MY Koh, YH AF Phuong Nguyen Kim, A-Young Jung, Jin Kyo Donahue, Kelly M. Jung, Chuleui Choi, Man-Yeon Koh, Young Ho TI The Biochemical Adaptations of Spotted Wing Drosophila (Diptera: Drosophilidae) to Fresh Fruits Reduced Fructose Concentrations and Glutathione-S Transferase Activities SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE spotted wing drosophila; food preference; fructose; glutathione-S transferase; carbohydrate metabolism ID SUZUKII; SUSCEPTIBILITY; INSECTICIDES; MELANOGASTER; ENHANCEMENT; INFESTATION; EVOLUTION; INVASION; PROTEIN; GENOME AB Spotted wing drosophila, Drosophila suzukii Matsumura, is an invasive and economically damaging pest in Europe and North America. The females have a serrated ovipositor that enables them to infest almost all ripening small fruits. To understand the physiological and metabolic basis of spotted wing drosophila food preferences for healthy ripening fruits, we investigated the biological and biochemical characteristics of spotted wing drosophila and compared them with those of Drosophila melanogaster Meigen. We found that the susceptibility to oxidative stressors was significantly increased in spotted wing drosophila compared with those of D. melanogaster. In addition, we found that spotted wing drosophila had significantly reduced glutathione-S transferase (GST) activity and gene numbers. Furthermore, fructose concentrations found in spotted wing drosophila were significantly lower than those of D. melanogaster. Our data strongly suggest that the altered food preferences of spotted wing drosophila may stem from evolutionary adaptations to fresh foods accompanied by alterations in carbohydrate metabolism and GST activities. C1 [Phuong Nguyen; Kim, A-Young; Koh, Young Ho] Hallym Univ, Ilsong Inst Life Sci, Dept Biomed Gerontol, Anyang 14066, Gyeonggi Do, South Korea. [Jung, Jin Kyo] Natl Inst Crop Sci, Dept Cent Area, Crop Cultivat & Environm Res Div, Suwon 441853, Gyeonggi Do, South Korea. [Donahue, Kelly M.; Choi, Man-Yeon] USDA ARS, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Jung, Chuleui] Andong Univ, Dept Plant Med, Andong 36729, Gyeong Sang Boo, South Korea. RP Koh, YH (reprint author), Hallym Univ, Ilsong Inst Life Sci, Dept Biomed Gerontol, Anyang 14066, Gyeonggi Do, South Korea. EM phuongnguyenvspt@gmail.com; kilenay@daum.net; jungjk@korea.kr; Kelly.Donahue@ars.usda.gov; cjung@andong.ac.kr; mychoi@ars.usda.gov; kohyh@hallym.ac.kr FU Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration [PJ011630042016]; Northwest Center for Small Fruits Research Grant, USDA-ARS FX We thank Drs. Peter Follett and Jana Lee for providing spotted wing drosophila strains. This work was carried out with the support of the Cooperative Research Program for Agriculture Science & Technology Development (Project title: Studies for biological characteristics of and control methods against the migratory locust, Locusta migratoria, Project No: PJ011630042016), Rural Development Administration, and supported in part by a Northwest Center for Small Fruits Research Grant, USDA-ARS. NR 32 TC 1 Z9 1 U1 2 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2016 VL 109 IS 2 BP 973 EP 981 DI 10.1093/jee/tow019 PG 9 WC Entomology SC Entomology GA DJ8XI UT WOS:000374497000065 PM 26921228 ER PT J AU Hau, C Reid, KF Wong, KF Chin, RJ Botto, TJ Eliasziw, M Bermudez, OI Fielding, RA AF Hau, C. Reid, K. F. Wong, K. F. Chin, R. J. Botto, T. J. Eliasziw, M. Bermudez, O. I. Fielding, R. A. TI COLLABORATIVE EVALUATION OF THE HEALTHY HABITS PROGRAM: AN EFFECTIVE COMMUNITY INTERVENTION TO IMPROVE MOBILITY AND COGNITION OF CHINESE OLDER ADULTS LIVING IN THE US SO JOURNAL OF NUTRITION HEALTH & AGING LA English DT Article DE Physical activity; mobility; cognition; older ethnic minorities; community engagement ID MINI-MENTAL-STATE; PHYSICAL PERFORMANCE; MEANINGFUL CHANGE; MUSCLE STRENGTH; SKELETAL-MUSCLE; CLINICAL-TRIAL; PUBLIC-HEALTH; DISABILITY; LIFE; FRAILTY AB Objectives: There is a growing demand to reduce ethnic health disparities. The Healthy Habits Program (HHP) was implemented to provide a community-based physical activity and education intervention for Chinese older adults living in Boston, Massachusetts. This study evaluated the HHP by assessing outcomes that are critical for maintaining independence of older persons. Design, setting & participants: Quantitative evaluation was performed on 50 Chinese older adults enrolled in the HHP. The community members were trained in data collection and management. Measurements: Cognition (Mini Mental State Examination (MMSE), Trail Making Test & Complex Walking Task), mobility (Short Physical Performance Battery (SPPB) & maximal gait speed), depressive symptoms (Patient Health Questionnaire-9), perceived disability (World Health Organization Disability Assessment 2.0), nutritional status (Mini Nutrition Assessment (R)), and strength (grip and leg strength) were assessed at baseline and at 6 months. All tests were translated into Chinese. Results: Of the 50 participants (mean age 68.4 years; 68% female), 78% achieved the goal of performing exercise >= 3 times/week. After 6 months, clinically meaningful improvements were observed in mobility (mean SPPB score changed from 10.3 to 11.1 points; p=0.01) and cognition (mean MMSE score changed from 26.0 to 27.8 points; p=0.001). There were also statistically significant improvements in executive function, depressive symptoms and perceived disability (p<0.05). Conclusion: Culturally sensitive community interventions, such as the HHP, are effective for improving mobility and cognition of Chinese older adults. This reveals the potential of promoting successful aging in minority populations through community settings, and should be advocated to reduce ethnic health disparities in the U.S. C1 [Hau, C.; Reid, K. F.; Fielding, R. A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. [Wong, K. F.; Chin, R. J.; Botto, T. J.] YMCA Greater Boston, Wang Branch, Boston, MA USA. [Eliasziw, M.; Bermudez, O. I.] Tufts Univ, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. RP Fielding, RA (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. EM roger.fielding@tufts.edu FU Asian Health Initiative at Tufts Medical Center; Wang Branch of the Young Men's Christian Association (YMCA); U.S. Department of Agriculture [58-1950-4-003]; Nutrition, Exercise Physiology, and Sarcopenia (NEPS) laboratory in the Jean Mayer USDA Human Nutrition Research Center on Aging (HNRCA) at Tufts University FX The Healthy Habits Program was funded by the Asian Health Initiative at Tufts Medical Center. This study was supported and conducted by the Wang Branch of the Young Men's Christian Association (YMCA), the U.S. Department of Agriculture, under agreement No. 58-1950-4-003, and the Nutrition, Exercise Physiology, and Sarcopenia (NEPS) laboratory in the Jean Mayer USDA Human Nutrition Research Center on Aging (HNRCA) at Tufts University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 43 TC 0 Z9 0 U1 5 U2 8 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1279-7707 EI 1760-4788 J9 J NUTR HEALTH AGING JI J. Nutr. Health Aging PD APR PY 2016 VL 20 IS 4 BP 391 EP 397 PG 7 WC Geriatrics & Gerontology; Nutrition & Dietetics SC Geriatrics & Gerontology; Nutrition & Dietetics GA DK1VF UT WOS:000374702200004 PM 26999238 ER PT J AU Sinha, I Karagoz, K Fogle, RL Hollenbeak, CS Zea, AH Arga, KY Stanley, AE Hawkes, WC Sinha, R AF Sinha, Indu Karagoz, Kubra Fogle, Rachel L. Hollenbeak, Christopher S. Zea, Arnold H. Arga, Kazim Y. Stanley, Anne E. Hawkes, Wayne C. Sinha, Raghu TI "Omics" of Selenium Biology: A Prospective Study of Plasma Proteome Network Before and After Selenized-Yeast Supplementation in Healthy Men SO OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; NORTH-AMERICAN MEN; INSULIN-RESISTANCE; PROSTATE-CANCER; INTEGRATIVE ANALYSIS; BINDING PROTEIN; PHOSPHOLIPASE-D; BREAST-CANCER; DISEASE; SERUM AB Low selenium levels have been linked to a higher incidence of cancer and other diseases, including Keshan, Chagas, and Kashin-Beck, and insulin resistance. Additionally, muscle and cardiovascular disorders, immune dysfunction, cancer, neurological disorders, and endocrine function have been associated with mutations in genes encoding for selenoproteins. Selenium biology is complex, and a systems biology approach to study global metabolomics, genomics, and/or proteomics may provide important clues to examining selenium-responsive markers in circulation. In the current investigation, we applied a global proteomics approach on plasma samples collected from a previously conducted, double-blinded placebo controlled clinical study, where men were supplemented with selenized-yeast (Se-Yeast; 300g/day, 3.8mol/day) or placebo-yeast for 48 weeks. Proteomic analysis was performed by iTRAQ on 8 plasma samples from each arm at baseline and 48 weeks. A total of 161 plasma proteins were identified in both arms. Twenty-two proteins were significantly altered following Se-Yeast supplementation and thirteen proteins were significantly changed after placebo-yeast supplementation in healthy men. The differentially expressed proteins were involved in complement and coagulation pathways, immune functions, lipid metabolism, and insulin resistance. Reconstruction and analysis of protein-protein interaction network around selected proteins revealed several hub proteins. One of the interactions suggested by our analysis, PHLD-APOA4, which is involved in insulin resistance, was subsequently validated by Western blot analysis. Our systems approach illustrates a viable platform for investigating responsive proteomic profile in before and after' condition following Se-Yeast supplementation. The nature of proteins identified suggests that selenium may play an important role in complement and coagulation pathways, and insulin resistance. C1 [Sinha, Indu; Sinha, Raghu] Penn State Univ, Coll Med, Dept Biochem & Mol Biol, 500 Univ Dr, Hershey, PA 17033 USA. [Fogle, Rachel L.; Hollenbeak, Christopher S.] Penn State Univ, Coll Med, Dept Surg, Hershey, PA USA. [Stanley, Anne E.] Penn State Univ, Coll Med, Dept Mass Spectrometry Core, Hershey, PA USA. [Karagoz, Kubra; Arga, Kazim Y.] Marmara Univ, Dept Bioengn, Istanbul, Turkey. [Zea, Arnold H.] LSU Hlth Sci Ctr, Stanley S, Scott Canc Ctr, New Orleans, LA USA. [Zea, Arnold H.] LSU Hlth Sci Ctr, Dept Microbiol Immunol & Parasitol, New Orleans, LA USA. [Hawkes, Wayne C.] Univ Calif Davis, USDA, ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Fogle, Rachel L.] Millersville Univ Pennsylvania, Dept Biol, Millersville, PA 17551 USA. RP Sinha, R (reprint author), Penn State Univ, Coll Med, Dept Biochem & Mol Biol, 500 Univ Dr, Hershey, PA 17033 USA. EM rus15@psu.edu NR 58 TC 3 Z9 3 U1 3 U2 5 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1536-2310 EI 1557-8100 J9 OMICS JI OMICS PD APR PY 2016 VL 20 IS 4 BP 202 EP 213 DI 10.1089/omi.2015.0187 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DK2QZ UT WOS:000374760800002 PM 27027327 ER PT J AU Allan-Perkins, E Manter, D Jung, G AF Allan-Perkins, E. Manter, D. Jung, G. TI Abundance of Sclerotinia homoeocarpa in the thatch and soil on organic and conventional golf courses SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Allan-Perkins, E.; Jung, G.] Univ Massachusetts, Amherst, MA 01003 USA. [Manter, D.] USDA ARS, Ft Collins, CO 80522 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 1 EP 1 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400002 ER PT J AU Da Silva, W De Jong, W Gray, S AF Da Silva, W. De Jong, W. Gray, S. TI Search for molecular markers in potato germplasm for PTNRD expression SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Gray, S.] ARS, USDA, EPPRU, Ithaca, NY USA. [Gray, S.] Cornell Univ, Sch Integrat Plant Sci Plant Pathol & Plant Micro, Ithaca, NY USA. [Da Silva, W.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY USA. [De Jong, W.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 2 EP 2 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400005 ER PT J AU Gorny, A Wang, X Pethybridge, S AF Gorny, A. Wang, X. Pethybridge, S. TI Development of species-specific PCR tests for detection and quantification of Meloidogyne hapla SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Gorny, A.; Pethybridge, S.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY USA. [Wang, X.] Cornell Univ, USDA ARS, Plant Protect Unit, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 2 EP 2 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400008 ER PT J AU Keach, J Daughtrey, M Bridgen, M Salgado-Salazar, C AF Keach, J. Daughtrey, M. Bridgen, M. Salgado-Salazar, C. TI Susceptibility of Impatiens species to downy mildew caused by Plasmopara obducens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Keach, J.] Cornell Univ, Plant Breeding & Genet, Ithaca, NY USA. [Bridgen, M.] Cornell Univ, LIHREC, Hort, Riverhead, NY USA. [Daughtrey, M.] Cornell Univ, LIHREC, Plant Pathol & Plant Microbe Biol, Riverhead, NY USA. [Salgado-Salazar, C.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 3 EP 3 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400012 ER PT J AU Ferguson, MH Clark, C Smith, B AF Ferguson, M. H. Clark, C. Smith, B. TI Xylella fastidiosa in rabbiteye blueberry in Louisiana is genetically similar to a strain found in Southern highbush blueberry in Georgia SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Ferguson, M. H.; Clark, C.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Smith, B.] USDA ARS, Southern Hort Lab, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 8 EP 8 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400031 ER PT J AU Fountain, J Bajaj, P Yang, L Pandey, M Nayak, S Kumar, V Jayale, A Chitikineni, A Lee, D Kemerait, R Varshney, R Guo, B AF Fountain, J. Bajaj, P. Yang, L. Pandey, M. Nayak, S. Kumar, V. Jayale, A. Chitikineni, A. Lee, D. Kemerait, R. Varshney, R. Guo, B. TI Comparative transcriptome analyses of Aspergillus flavus isolates under different oxidative stresses and culture media SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Fountain, J.; Yang, L.; Kemerait, R.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Fountain, J.; Guo, B.] USDA ARS, Tifton, GA 31793 USA. [Bajaj, P.; Pandey, M.; Nayak, S.; Kumar, V.; Jayale, A.; Chitikineni, A.; Varshney, R.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Lee, D.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 8 EP 8 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400033 ER PT J AU Ji, X Guo, X Fountain, J Pandey, M Nayak, S Wang, H Holbrook, C Varshney, R Guo, B AF Ji, X. Guo, X. Fountain, J. Pandey, M. Nayak, S. Wang, H. Holbrook, C. Varshney, R. Guo, B. TI Identification of genomic regions controlling resistance to aflatoxin contamination in a peanut recombinant inbred line population (Tifrunner x GT-C20) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Ji, X.; Guo, X.; Fountain, J.; Wang, H.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Pandey, M.; Nayak, S.; Holbrook, C.; Varshney, R.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. USDA ARS, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 9 EP 10 PG 2 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400039 ER PT J AU Pelham, S Holbrook, C Guo, B Chu, Y Ozias-Akins, P Culbreath, A AF Pelham, S. Holbrook, C. Guo, B. Chu, Y. Ozias-Akins, P. Culbreath, A. TI Comparison of four RIL mapping populations of peanut for field response to late leaf spot SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Pelham, S.; Culbreath, A.] Univ Georgia, Plant Pathol, Tifton, GA USA. [Holbrook, C.; Guo, B.] USDA ARS, Tifton, GA 31793 USA. [Chu, Y.; Ozias-Akins, P.] Univ Georgia, Hort, Tifton, GA USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 13 EP 13 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400055 ER PT J AU Shrestha, S Cochran, A Lamour, K Mengistu, A Castro-Rocha, A Kelly, H AF Shrestha, S. Cochran, A. Lamour, K. Mengistu, A. Castro-Rocha, A. Kelly, H. TI Population structure of Cercospora sojina collected from different soybean cultivars in Milan and Jackson, Tennessee SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Shrestha, S.; Lamour, K.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37901 USA. [Cochran, A.; Kelly, H.] Univ Tennessee, Dept Entomol & Plant Pathol, Jackson, TN USA. [Mengistu, A.] USDA ARS, Jackson, TN USA. [Castro-Rocha, A.] Univ Autonoma Cd Juarez, Dept Ciencias Quim Biol, Chihuahua, Mexico. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 14 EP 14 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400061 ER PT J AU Wang, H Guo, X Pandey, M Ji, X Holbrook, C Culbreath, A Kemerait, R Varshney, R Guo, B AF Wang, H. Guo, X. Pandey, M. Ji, X. Holbrook, C. Culbreath, A. Kemerait, R. Varshney, R. Guo, B. TI Phenotypic assessments of peanut nested association mapping (NAM) populations SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Wang, H.; Guo, X.; Ji, X.; Culbreath, A.; Kemerait, R.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Pandey, M.; Varshney, R.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Holbrook, C.; Guo, B.] USDA ARS, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 15 EP 15 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400066 ER PT J AU Yang, L Fountain, J Chu, Y Ni, X Lee, D Kemerait, R Guo, B AF Yang, L. Fountain, J. Chu, Y. Ni, X. Lee, D. Kemerait, R. Guo, B. TI Differential accumulation of reactive oxygen and nitrogen species in maize lines with contrasting drought tolerance and aflatoxin resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Northeastern-Division of American-Phytopathological-Society (APS) in conjunction with the Northeast Plant, Pest, and Soil Conference CY JAN 03-07, 2016 CL Philadelphia, PA SP Amer Phytopathol Soc, NE Div C1 [Yang, L.; Fountain, J.; Kemerait, R.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Ni, X.; Guo, B.] USDA ARS, Tifton, GA 31793 USA. [Lee, D.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA USA. [Chu, Y.] Univ Georgia, Dept Hort, Tifton, GA USA. NR 0 TC 1 Z9 1 U1 2 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 2 SU 2 BP 16 EP 16 PG 1 WC Plant Sciences SC Plant Sciences GA DK0FG UT WOS:000374587400073 ER PT J AU Greenlee, JJ Smith, JD Hamir, AN AF Greenlee, Justin J. Smith, Jodi D. Hamir, Amir N. TI Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Prion protein; PRNP; Scrapie; Sheep; Transmissible spongiform encephalopathy ID NATURAL SCRAPIE; PRION PROTEIN; UNITED-STATES; TRANSMISSION; FLOCK; ASSOCIATION; SAMPLES; CATTLE; GENE AB Scrapie is a transmissible spongiform encephalopathy that can be transmitted amongst susceptible sheep. The prion protein gene (PRNP) profoundly influences the susceptibility of sheep to the scrapie agent. This study reports the failure to detect PrPSc in nervous or lymphoid tissues of Suffolk sheep of the PRNP ARQ/ARR genotype after oral inoculation with a U.S. scrapie isolate. Lambs were inoculated within the first 24 h of birth with 1 ml of a 10% (wt./vol.) brain homogenate derived from a clinically affected ARQ/ARQ sheep. The inoculated sheep were observed daily throughout the experiment for clinical signs suggestive of scrapie until they were necropsied at 86 months post inoculation. Tissues were collected for examination by immunohistochemistry and enzyme immunoassay, but all failed to demonstrate evidence of scrapie infection. Neonatal sheep of the ARQ/ARQ genotype receiving the same inoculum developed scrapie within 24 months. Lambs of the ARQ/ARR genotype that received the same inoculum by intracranial inoculation develop scrapie with a prolonged incubation period and with abnormal prion present within the central nervous system, but not peripheral lymphoid tissues. Results of this study suggest that ARQ/ARR sheep are resistant to oral infection with the scrapie isolate used even during the neonatal period. Published by Elsevier Ltd. C1 [Greenlee, Justin J.; Smith, Jodi D.; Hamir, Amir N.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. [Smith, Jodi D.] Iowa State Univ, Dept Vet Pathol, Coll Vet Med, Ames, IA 50011 USA. RP Greenlee, JJ (reprint author), ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM justin.greenlee@ars.usda.gov FU congressionally appropriated funds FX The authors thank Martha Church, Joe Lesan, Leisa Mandell, and Trudy Tatum for providing technical support to this project. This research was funded in its entirety by congressionally appropriated funds to the United States Department of Agriculture, Agriculture Research Service. The funders of the work did not influence study design, data collection and analysis, decision to publish, or preparation of the manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 25 TC 0 Z9 0 U1 2 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD APR PY 2016 VL 105 BP 188 EP 191 DI 10.1016/j.rvsc.2016.02.016 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA DJ7CL UT WOS:000374369400033 PM 27033930 ER PT J AU Kolka, RK Murdiyarso, D Kauffman, JB Birdsey, RA AF Kolka, R. K. Murdiyarso, D. Kauffman, J. B. Birdsey, R. A. TI Tropical wetlands, climate, and land-use change: adaptation and mitigation opportunities SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Editorial Material ID CARBON; FORESTS C1 [Kolka, R. K.] USDA Forest Serv, Northern Res Stn, Grand Rapids, MN USA. [Murdiyarso, D.; Kauffman, J. B.] Ctr Int Forestry Res, Bogor 16115, Indonesia. [Murdiyarso, D.] Bogor Agr Univ, Dept Geophys & Meteorol, Bogor 16680, Indonesia. [Kauffman, J. B.] Oregon State Univ, Corvallis, OR 97331 USA. [Birdsey, R. A.] USDA Forest Serv, Northern Res Stn, Newtown Sq, PA USA. RP Kolka, RK (reprint author), USDA Forest Serv, Northern Res Stn, Grand Rapids, MN USA. EM rkolka@fs.fed.us NR 23 TC 1 Z9 1 U1 14 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 107 EP 112 DI 10.1007/s11273-016-9487-x PG 6 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800001 ER PT J AU Hribljan, JA Suarez, E Heckman, KA Lilleskov, EA Chimner, RA AF Hribljan, John A. Suarez, Esteban Heckman, Katherine A. Lilleskov, Erik A. Chimner, Rodney A. TI Peatland carbon stocks and accumulation rates in the Ecuadorian paramo SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Peatland; Paramo; Carbon; Accumulation rates; Tuberas ID HIGH-ALTITUDE PEATLANDS; CLIMATE-CHANGE; TROPICAL ANDES; ORGANIC-CARBON; SOUTH-AMERICA; BOG GROWTH; RADIOCARBON; CALIBRATION; FUTURE; LIMITS AB The paramo is a high altitude tropical Andean ecosystem that contains peatlands with thick horizons of carbon (C) dense soils. Soil C data are sparse for most of the paramo, especially in peatlands, which limits our ability to provide accurate regional and country wide estimates of C storage. Therefore, the objective of our research was to quantify belowground C stocks and accumulation rates in paramo peatland soils in two regions of northeastern Ecuador. Peatland soil cores were collected from Antisana Ecological Reserve and Cayambe-Coca National Park. We measured soil C densities and C-14 dates to estimate soil accumulation rates. The mean peatland soil depth across both regions was 3.8 m and contained an estimated mean C storage of 1282 Mg ha(-1). Peatlands older than 3000 cal. year BP had a mean long-term C accumulation rate of 26 g m(-2) year(-1), with peatlands younger than 500 cal. year BP displaying mean recent rates of C accumulation of 134 g m(-2) year(-1). These peatlands also receive large inputs of mineral material, predominantly from volcanic deposition, that has created many interbedded non-peat mineral soil horizons that contained 48 % of the soil C. Because of large C stocks in Ecuadorian mountain peatlands and the potential disturbance from land use and climate change, additional studies are need to provide essential baseline assessments and estimates of C storage in the Andes. C1 [Hribljan, John A.; Chimner, Rodney A.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Suarez, Esteban] Univ San Francisco Quito, Colegio Ciencias Biol & Ambient, Quito, Ecuador. [Heckman, Katherine A.; Lilleskov, Erik A.] USDA Forest Serv, Northern Res Stn, Houghton, MI 49931 USA. RP Hribljan, JA (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. EM jahriblj@mtu.edu OI Suarez, Esteban/0000-0003-0425-8933 FU Antisana Ecological Reserve; Cayambe-Coca National Park; Sustainable Wetlands Adaptation and Mitigation program (SWAMP); USDA Forest Service; Lawrence Livermore National Laboratory; Michigan Technological University; Universidad San Francisco de Quito FX The authors thank Alexander N. Brown, Ellie Crane, Francisco Cuesta, Christa M. Luokkala, Austin Meyer, Priscilla Muriel, Manuel Peralvo, Maria Elisa Sanchez, and Jose Schreckinger for field assistance. We thank Ellen Beller, Aric Devens, Christa M. Luokkala, Jennifer Eikenberry, Beth Spedowski, and Tim Veverica for laboratory assistance. A special thank you to Craig Wayson and Ana Maria Planas (SilvaCarbon) and Erin Cary (USFS International Programs) for logistic support. The site map was created by Jose Schreckinger. The authors thank the Ministry of the Environment of Ecuador for issuing the research permit (N.-04-2014-IC-FLO-DPAPMA). This project would not have been possible without the support of the Antisana Ecological Reserve and Cayambe-Coca National Park and the park rangers for their collaboration during this research. This project was supported by the Sustainable Wetlands Adaptation and Mitigation program (SWAMP). Radiocarbon analysis was supported by the Radiocarbon Collaborative, which is jointly sponsored by the USDA Forest Service, Lawrence Livermore National Laboratory, and Michigan Technological University. Additional financial support for ES was provided by the Chancellor Grants and Collaboration Grants of Universidad San Francisco de Quito. NR 46 TC 2 Z9 2 U1 14 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 113 EP 127 DI 10.1007/s11273-016-9482-2 PG 15 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800002 ER PT J AU Stringer, CE Trettin, CC Zarnoch, SJ AF Stringer, Christina E. Trettin, Carl C. Zarnoch, Stanley J. TI Soil properties of mangroves in contrasting geomorphic settings within the Zambezi River Delta, Mozambique SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Blue carbon; Forested wetland; Organic matter; Soil carbon; Soil nitrogen; Zambezi River Delta ID ORGANIC-MATTER; CARBON STOCKS; FORESTS; RESTORATION; ECOSYSTEMS; BAY AB Mangroves are well-known for their numerous ecosystem services, including sequestering a significant carbon stock, with soils accounting for the largest pool. The soil carbon pool is dependent on the carbon content and bulk density. Our objective was to assess the spatial variability of mangrove soil physical and chemical properties within the Zambezi River Delta and determine whether it may be associated with geomorphic setting. Plots were classified as one of four geomorphic settings: seaward fringe, creek, riverine, and interior. Additionally, we attempted to determine the source(s) of organic matter contributing to the soil carbon pool and any associated spatial variability therein. Many statistically significant differences were shown with depth and setting. However, variability of the measured characteristics was low when compared to other mangrove settings. Mean carbon concentrations ranged from 1.38 to 2.38 % C and mean bulk density values ranged from 0.75 to 1.02 g cm(-3). Stable isotopic signatures showed that the organic matter is likely a mix of mangrove and marine sources, with mangrove-derived sources contributing 42-58 %. C1 [Stringer, Christina E.; Trettin, Carl C.] USDA Forest Serv, Southern Res Stn, Ctr Forested Wetlands Res, Cordesville, SC USA. [Zarnoch, Stanley J.] USDA Forest Serv, Southern Res Stn, Forest Inventory & Anal, Clemson, SC USA. RP Stringer, CE (reprint author), USDA Forest Serv, Southern Res Stn, Ctr Forested Wetlands Res, Cordesville, SC USA. EM christinaestringer@fs.fed.us FU USDA Forest Service; USAID FX USDA Forest Service, USAID. NR 38 TC 1 Z9 1 U1 13 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 139 EP 152 DI 10.1007/s11273-015-9478-3 PG 14 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800004 ER PT J AU Tang, WW Feng, WP Jia, MJ Shi, JY Zuo, HF Trettin, CC AF Tang, Wenwu Feng, Wenpeng Jia, Meijuan Shi, Jiyang Zuo, Huifang Trettin, Carl C. TI The assessment of mangrove biomass and carbon in West Africa: a spatially explicit analytical framework SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Mangrove biomass; Canopy height; Forest inventory; West Africa ID ABOVEGROUND BIOMASS; ORGANIC-CARBON; ELEVATION DATA; FORESTS; DYNAMICS; PRODUCTIVITY; ICESAT/GLAS; ECOSYSTEMS; HEIGHT; FIELD AB Mangrove forests are highly productive and have large carbon sinks while also providing numerous goods and ecosystem services. However, effective management and conservation of the mangrove forests are often dependent on spatially explicit assessments of the resource. Given the remote and highly dispersed nature of mangroves, estimation of biomass and carbon in mangroves through routine field-based inventories represents a challenging task which is impractical for large-scale planning and assessment. Alternative approaches based on geospatial technologies are needed to support this estimation in large areas. However, spatial data processing and analysis approaches used in this estimation of mangrove biomass and carbon have not been adequately investigated. In this study, we present a spatially explicit analytical framework that integrate remotely sensed data and spatial analyses approaches to support the estimation of mangrove biomass and carbon stock and their spatial patterns in West Africa. Forest canopy height derived from SRTM and ICESat/GLAS data was used to estimate mangrove biomass and carbon in nine West African countries. We developed a geospatial software toolkit that implemented the proposed framework. The spatial analysis framework and software toolkit provide solid support for the estimation and relative comparisons of mangrove-related metrics. While the mean canopy height of mangroves in our study area is 10.2 m, the total biomass and carbon were estimated as 272.56 and 136.28 Tg. Nigeria has the highest total mangrove biomass and carbon in the nine countries, but Cameroon is the country with the largest mean biomass and carbon density. The resulting spatially explicit distributions of mangrove biomass and carbon hold great potential in guiding the strategic planning of large-scale field-based assessment of mangrove forests. This study demonstrates the utility of online geospatial data and spatial analysis as a feasible solution for estimating the distribution of mangrove biomass and carbon at larger or smaller scales. C1 [Tang, Wenwu; Feng, Wenpeng; Jia, Meijuan; Shi, Jiyang; Zuo, Huifang] Univ N Carolina, Ctr Appl Geog Informat Sci, Charlotte, NC 28223 USA. [Tang, Wenwu; Feng, Wenpeng; Jia, Meijuan; Shi, Jiyang; Zuo, Huifang] Univ N Carolina, Dept Geog & Earth Sci, Charlotte, NC 28223 USA. [Trettin, Carl C.] US Forest Serv, Ctr Forested Wetlands Res, Cordesville, SC 29434 USA. RP Tang, WW (reprint author), Univ N Carolina, Ctr Appl Geog Informat Sci, Charlotte, NC 28223 USA. EM WenwuTang@uncc.edu FU U.S. Agency for International Development (USAID) West Africa Mission FX This study was made possible with support from the U.S. Agency for International Development (USAID) West Africa Mission that was managed through the International Programs Office of the USDA Forest Service. The contents are the responsibility of the authors and do not necessarily reflect the opinion of USAID or the U.S. Government. We appreciate the help from Dr. Marc Simard and Dr. Lola Fatoyinbo, NASA, in the application of the SRTM data, and Mr. Gray G. Tappan and Mr. John Hutchinson from USGS for kindly sharing their datasets to support this study. We owe our gratitude to Dr. Eric Wolanski, Dr. Randy Kolka, Dr. Daniel Murdiyarso, and two anonymous reviewers for their constructive comments. NR 38 TC 1 Z9 1 U1 12 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 153 EP 171 DI 10.1007/s11273-015-9474-7 PG 19 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800005 ER PT J AU Trettin, CC Stringer, CE Zarnoch, SJ AF Trettin, Carl C. Stringer, Christina E. Zarnoch, Stanley J. TI Composition, biomass and structure of mangroves within the Zambezi River Delta SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Forest inventory; Mangrove biomass; Zambezi River Delta ID CARBON STOCKS; FORESTS; ECOSYSTEMS; PRODUCTIVITY; MOZAMBIQUE; DYNAMICS; PATTERNS; BAY AB We used a stratified random sampling design to inventory the mangrove vegetation within the Zambezi River Delta, Mozambique, to provide a basis for estimating biomass pools. We used canopy height, derived from remote sensing data, to stratify the inventory area, and then applied a spatial decision support system to objectively allocate sample plots among five strata. Height and diameter were measured on overstory trees, saplings and standing dead trees in nested plots, and biomass was calculated using allometric equations. Each of the eight mangrove species occurring in Mozambique exist within the Delta. They are distributed in heterogeneous mixtures within each of the five canopy height classes, not reflecting obvious zonation. Overstory trees averaged approximately 2000 trees ha(-1), and average basal area ranged from 14 to 41 m(2) ha(-1) among height classes. The composition of the saplings tended to mirror the overstory, and the diameter frequency distributions suggest all-aged stands. Above-ground biomass ranged from 111 to 483 Mg ha(-1) with 95 % confidence interval generally within 15 % of the height class mean. Despite over 3000 trees ha(-1) in the small-tree component, 92 % of the vegetation biomass is in the overstory live trees. The objective inventory design proved effective in estimating forest biomass within the 30,267 ha mangrove forest. C1 [Trettin, Carl C.; Stringer, Christina E.] USDA Forest Serv, Ctr Forested Wetlands Res, Southern Res Stn, Cordesville, SC USA. [Zarnoch, Stanley J.] USDA Forest Serv, Forest Inventory & Anal, Southern Res Stn, Clemson, SC USA. RP Trettin, CC (reprint author), USDA Forest Serv, Ctr Forested Wetlands Res, Southern Res Stn, Cordesville, SC USA. EM ctrettin@fs.fed.us FU US Agency for International Development; US Dept. of Agriculture, Forest Service FX Funds for this work were provided by the US Agency for International Development and the US Dept. of Agriculture, Forest Service. NR 35 TC 2 Z9 2 U1 9 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 173 EP 186 DI 10.1007/s11273-015-9465-8 PG 14 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800006 ER PT J AU Nam, VN Sasmito, SD Murdiyarso, D Purbopuspito, J MacKenzie, RA AF Vien Ngoc Nam Sasmito, Sigit D. Murdiyarso, Daniel Purbopuspito, Joko MacKenzie, Richard A. TI Carbon stocks in artificially and naturally regenerated mangrove ecosystems in the Mekong Delta SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Blue carbon; Climate mitigation; Coastal management; Ecosystem C stocks; Hydrogeomorphic; Restoration; Vietnam ID CLIMATE-CHANGE MITIGATION; MUI CA MAU; EARTH-OBSERVATION; GROUND BIOMASS; SEA-LEVEL; FORESTS; ALLOMETRY; PRODUCTIVITY; WETLANDS; VIETNAM AB Mangrove forests cover a small fraction of the earth's surface, but contribute disproportionately to ecosystem services, including carbon (C) storage. These forests are being rapidly degraded as demand for economic development grows. In recognition of the multiple benefits of mangrove forests, rehabilitation of degraded forests is being carried out in many regions. This study assesses the potential for restored mangrove forests in Vietnam to sequester and store C, by characterizing two different mangrove restoration areas in the Mekong Delta region. The Can Gio Mangrove Biospheres Reserve (CGMBR) in Ho Chi Minh City was highly degraded during the Vietnam War and was subsequently replanted between 1978 and 1998. The Kien Vang Protection Forest (KVPF) in Ca Mau Province was similarly degraded during the war, but unlike CGMBR, it has experienced natural regeneration over the last 35 years. We find that vegetation structure between two sites are not different significantly, though CGMBR has richer mangrove species diversity than KVPF. The mean of total ecosystem C stocks in planted mangroves of CGMBR (889 +/- A 111 MgC ha(-1)) is not significantly different compare to natural regeneration forests of KVPF (844 +/- A 58 MgC ha(-1)). Our findings suggest that after 35 years, both anthropogenically and naturally regenerated mangroves appear to store similar levels of C. C1 [Vien Ngoc Nam] Nong Lam Univ, Ho Chi Minh City 70999, Vietnam. [Sasmito, Sigit D.; Murdiyarso, Daniel] Ctr Int Forestry Res, Jalan CIFOR, Bogor 16115, Indonesia. [Sasmito, Sigit D.] Charles Darwin Univ, Res Inst Environm & Livelihoods, Darwin, NT 0810, Australia. [Murdiyarso, Daniel] Bogor Agr Univ, Dept Geophys & Meteorol, Bogor 16680, Indonesia. [Purbopuspito, Joko] Sam Ratulangi Univ, Dept Soil Sci, Manado 95115, Indonesia. [MacKenzie, Richard A.] USDA Forest Serv, Pacific Southwest Res Stn, Inst Pacific Isl Forestry, Hilo, HI 96721 USA. RP Nam, VN (reprint author), Nong Lam Univ, Ho Chi Minh City 70999, Vietnam.; Sasmito, SD (reprint author), Ctr Int Forestry Res, Jalan CIFOR, Bogor 16115, Indonesia. EM drvnnam@gmail.com; s.sasmito@cgiar.org RI Purbopuspito, Joko/P-1165-2015 OI Purbopuspito, Joko/0000-0002-4631-596X FU United States Agency for International Development (USAID) [MTO. 069018] FX The research was conducted under the Sustainable Wetland Adaptation and Mitigation Program (SWAMP), supported by the United States Agency for International Development (USAID) (MTO. 069018). We would like to thank the authorities of Can Gio District Peoples Committee, Can Gio Mangrove Biosphere Reserve of Ho Chi Minh City, Department of Agriculture and Rural Development of Ca Mau province and the KVPF Management Board who facilitated the collection of field data. We would like to thank Kemen Austin and Imam Basuki provided useful comments on the manuscript. This paper is number #25 in the SWAMP peer-reviewed publication series (www.cifor.org/swamp). NR 48 TC 2 Z9 2 U1 9 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 231 EP 244 DI 10.1007/s11273-015-9479-2 PG 14 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800010 ER PT J AU MacKenzie, RA Foulk, PB Klump, JV Weckerly, K Purbospito, J Murdiyarso, D Donato, DC Nam, VN AF MacKenzie, Richard A. Foulk, Patra B. Klump, J. Val Weckerly, Kimberly Purbospito, Joko Murdiyarso, Daniel Donato, Daniel C. Vien Ngoc Nam TI Sedimentation and belowground carbon accumulation rates in mangrove forests that differ in diversity and land use: a tale of two mangroves SO WETLANDS ECOLOGY AND MANAGEMENT LA English DT Article DE Mangroves; Sea level rise; Sedimentation rates; Vertical accretion; Belowground carbon accumulation; Pb-210; Climate change ID SEA-LEVEL RISE; COASTAL WETLAND VULNERABILITY; LOWER MEKONG RIVER; INTERSPECIFIC COMPETITION; ECOSYSTEM FUNCTION; TROPICAL PACIFIC; EUROPEAN BEECH; CLIMATE-CHANGE; NORWAY SPRUCE; SALT-MARSH AB Increased sea level is the climate change effect expected to have the greatest impact on mangrove forest survival. Mangroves have survived extreme fluctuations in sea level in the past through sedimentation and belowground carbon (C) accumulation, yet it is unclear what factors may influence these two parameters. We measured sedimentation, vertical accretion, and belowground C accumulation rates in mangrove forests from the Republic of Palau and Vietnam to examine how diversity (high-Vietnam vs. low-Palau), land use, and location (fringe vs. interior) might influence these parameters. Land use in this study was identified as disturbance and restoration for all mangrove forests sampled in Palau and Vietnam, respectively. Vertical accretion rates were significantly greater in Vietnam (2.44 +/- A 1.38 cm/year) than Palau mangrove forests (0.47 +/- A 0.08 cm/year; p < 0.001, F-1,F-17 = 24.96). Vertical accretion rates were positively correlated to diversity (R = 0.43, p < 0.05). However, stronger correlations of accretion to bulk density (R = 0.64, p < 0.01) and significantly higher bulk densities in Vietnamese (0.67 +/- A 0.04 g/cm(3)) than Palau mangroves (0.30 +/- A 0.03 g/cm(3); p < 0.001, F-1,F-17 = 54.4) suggests that suspended sediments played a greater role in mangrove forest floor maintenance relative to sea level rise. Average vertical accretion rates were similar between naturally colonized (1.01 +/- A 0.10 cm/year) and outplanted sites (1.06 +/- A 0.05 cm/year) and between fringe (1.06 +/- A 0.12 cm/year) and interior mangrove (0.99 +/- A 0.09 cm/year) in Vietnam. In Palau, vertical accretion rates did not differ between disturbed (0.42 +/- A 0.11 cm/year) and undisturbed (0.51 +/- A 0.13 cm/year) mangrove forests and were higher in fringe (0.61 +/- A 0.15 cm/year) than interior sites (0.33 +/- A 0.09 cm/year; p = 0.1, F-1,F-7 = 3.45). Belowground C accumulation rates did not differ between any factors examined. C accumulation rates (69-602 gC/m(2)/year) were similar to those reported elsewhere in the literature and suggest that intact coastal ecosystems play an important role in the global C cycle, sequestering C at rates that are 10-20x greater than upland forests. Assuming vertical accretion rates measured using Pb-210 are an effective proxy for surface elevation, the Vietnamese and Palauan mangroves appear to be keeping up with current rates of sea level rise. C1 [MacKenzie, Richard A.; Foulk, Patra B.] Inst Pacific Isl Forestry, USDA Forest Serv, Pacific Southwest Res Ctr, 60 Nowelo St, Hilo, HI 96720 USA. [Klump, J. Val; Weckerly, Kimberly] Univ Wisconsin, Sch Freshwater Sci, 600 E Greenfield Ave, Milwaukee, WI 53201 USA. [Purbospito, Joko] Sam Ratulangi Univ, Dept Soil Sci, Manado 95115, Indonesia. [Murdiyarso, Daniel] Ctr Int Forestry Res, Bogor 16115, Indonesia. [Donato, Daniel C.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Vien Ngoc Nam] Nong Lom Univ, Fac Forestry, Ho Chi Minh City, Vietnam. RP MacKenzie, RA (reprint author), Inst Pacific Isl Forestry, USDA Forest Serv, Pacific Southwest Res Ctr, 60 Nowelo St, Hilo, HI 96720 USA. EM rmackenzie@fs.fed.us FU US Agency for International Development; USDA Forest Service's Pacific Southwest Research Station FX We thank Truong Van Vinh, Lucas Isechal, Omekreal Sadang, and Caitlynn Kryss for their assistance in collecting data from Vietnam and Palau. We also thank Maybeleen Apwong and James Akau for their lab assistance. Ken Krauss and Dan Friess as well two anonymous reviewers provide comments that significantly contributed to the quality of this manuscript. Funding for this project was provided the US Agency for International Development and the USDA Forest Service's Pacific Southwest Research Station. NR 89 TC 2 Z9 2 U1 24 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0923-4861 EI 1572-9834 J9 WETL ECOL MANAG JI Wetl. Ecol. Manag. PD APR PY 2016 VL 24 IS 2 SI SI BP 245 EP 261 DI 10.1007/s11273-016-9481-3 PG 17 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK0HF UT WOS:000374592800011 ER PT J AU Blesson, CS Schutt, AK Balakrishnan, MP Pautler, RG Pedersen, SE Sarkar, P Gonzales, D Zhu, G Marini, JC Chacko, SK Yallampalli, U Yallampalli, C AF Blesson, Chellakkan S. Schutt, Amy K. Balakrishnan, Meena P. Pautler, Robia G. Pedersen, Steen E. Sarkar, Poonam Gonzales, Daniel Zhu, Gang Marini, Juan C. Chacko, Shaji K. Yallampalli, Uma Yallampalli, Chandra TI Novel lean type 2 diabetic rat model using gestational low-protein programming SO AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY LA English DT Article; Proceedings Paper CT 36th Annual Pregnancy Meeting of the Society-for-Maternal-Fetal-Medicine (SMFM) CY FEB 01-08, 2016 CL Atlanta, GA SP Soc Maternal Fetal Med DE gestational programming; glucose intolerance; insulin resistance; lean diabetes; type 2 diabetes ID INSULIN-RESISTANCE; MELLITUS; ORIGINS; MECHANISMS; GLUCOSE; OBESITY; INDIA AB BACKGROUND: Type 2 diabetes (T2D) in lean individuals is not well studied and up to 26% of diabetes occurs in these individuals. Although the cause is not well understood, it has been primarily attributed to nutritional issues during early development. OBJECTIVE: Our objective was to develop a lean T2D model using gestational low-protein (LP) programming. STUDY DESIGN: Pregnant rats were fed control (20% protein) or isocaloric LP (6%) diet from gestational day 4 until delivery. Standard diet was given to dams after delivery and to pups after weaning. Glucose tolerance test was done at 2, 4, and 6 months of age. Magnetic resonance imaging of body fat for females was done at 4 months. Rats were sacrificed at 4 and 8 months of age and their perigonadal, perirenal, inguinal, and brown fat were weighed and expressed relative to their body weight. Euglycemic-hyperinsulinemic clamp was done around 6 months of age. RESULTS: Male and female offspring exposed to a LP diet during gestation developed glucose intolerance and insulin resistance (IR). Further, glucose intolerance progressed with increasing age and occurred earlier and was more severe in females when compared to males. Euglycemic-hyperinsulinemic clamp showed whole body IR in both sexes, with females demonstrating increased IR compared to males. LP females showed a 4.5-fold increase in IR while males showed a 2.5-fold increase when compared to their respective controls. Data from magnetic resonance imaging on female offspring showed no difference in the subcutaneous, inguinal, and visceral fat content. We were able to validate this observation by sacrificing the rats at 4 and 8 months and measuring total body fat content. This showed no differences in body fat content between control and LP offspring in either males or females. Additionally, diabetic rats had a similar body mass index to that of the controls. CONCLUSION: LP gestational programming produces a progressively worsening T2D model in rats with a lean phenotype without obesity. C1 [Blesson, Chellakkan S.; Schutt, Amy K.; Balakrishnan, Meena P.; Yallampalli, Uma; Yallampalli, Chandra] Baylor Coll Med, Dept Obstet & Gynecol, Houston, TX 77030 USA. [Pautler, Robia G.; Pedersen, Steen E.; Gonzales, Daniel] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA. [Pautler, Robia G.] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA. [Pautler, Robia G.] Baylor Coll Med, Dept Radiol, Houston, TX 77030 USA. [Sarkar, Poonam; Marini, Juan C.; Chacko, Shaji K.; Yallampalli, Uma] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Marini, Juan C.] Baylor Coll Med, Dept Crit Care Med, Houston, TX 77030 USA. [Pautler, Robia G.; Pedersen, Steen E.; Sarkar, Poonam; Gonzales, Daniel] Texas Childrens Hosp, Small Anim Imaging Facil, Houston, TX 77030 USA. [Marini, Juan C.; Chacko, Shaji K.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Zhu, Gang] Bruker Corp, Houston, TX USA. RP Yallampalli, C (reprint author), Baylor Coll Med, Dept Obstet & Gynecol, Houston, TX 77030 USA. EM cyallamp@bcm.edu FU NHLBI NIH HHS [R01 HL102866, R01 HL058144, HL58144, HL102866] NR 35 TC 0 Z9 0 U1 0 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0002-9378 EI 1097-6868 J9 AM J OBSTET GYNECOL JI Am. J. Obstet. Gynecol. PD APR PY 2016 VL 214 IS 4 AR 540.e1-e7 DI 10.1016/j.ajog.2016.02.004 PG 7 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA DL2EY UT WOS:000375447400033 PM 26874300 ER PT J AU Stark, JC Novy, RG Whitworth, JL Knowles, NR Pavek, MJ Thornton, M Spear, R Brown, CR Charlton, BA Sathuvalli, V Yilma, S Olsen, N Brandt, TL AF Stark, J. C. Novy, R. G. Whitworth, J. L. Knowles, N. R. Pavek, M. J. Thornton, M. Spear, R. Brown, C. R. Charlton, B. A. Sathuvalli, V. Yilma, S. Olsen, N. Brandt, T. L. TI Mountain Gem Russet: a Potato Variety with High Early and Full Season Yield Potential and Excellent Fresh Market and Early Processing Characteristics SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Variety; Breeding; Processing ID GERMPLASM; RESISTANCE; TRIALS AB Mountain Gem Russet is a potato variety notable for having both high early and full-season yields of oblong-long, medium-russeted tubers with higher protein content than those of standard potato varieties. Mountain Gem Russet has greater resistance to soft rot, tuber late blight, growth cracks and second growth than Russet Burbank. Mountain Gem Russet produced greater total and U.S. No. 1 yields than Ranger Russet and Russet Norkotah in early-season trials conducted in Idaho, Oregon and Washington over a 3 year period. In full-season trials conducted in five western states over a 4 year period, average total and U.S. No. 1 yields for Mountain Gem Russet were almost always higher than Ranger Russet and Russet Burbank, with specific gravities averaging between these two standard cultivars. It maintains acceptable reducing sugar concentrations and fry quality when stored at 8.9 degrees C and has displayed a high level of fry color uniformity from tuber stem to bud end providing good potential for early processing. High fresh merit ratings and taste panel sensory scores comparable to Russet Burbank indicate good fresh market potential for Mountain Gem Russet. It was released in 2015 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington, and is a product of the Pacific Northwest Potato Variety (Tri-State) Development Program. C1 [Stark, J. C.] Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. [Novy, R. G.; Whitworth, J. L.] USDA ARS, Aberdeen R&E Ctr, Aberdeen, ID 83210 USA. [Knowles, N. R.; Pavek, M. J.] Washington State Univ, Pullman, WA 99164 USA. [Thornton, M.] Univ Idaho, Southwest IdahoR&E Ctr, Parma, ID 83660 USA. [Spear, R.] McCain Foods, Burley, ID 83318 USA. [Brown, C. R.] ARS, USDA, Prosser, WA 99350 USA. [Charlton, B. A.] Oregon State Univ, Klamath Basin R&E Ctr, Klamath Falls, OR 97603 USA. [Sathuvalli, V.] Oregon State Univ, Hermiston Agr R&E Ctr, Hermiston, OR 97838 USA. [Yilma, S.] Oregon State Univ, Corvallis, OR 97331 USA. [Olsen, N.; Brandt, T. L.] Univ Idaho, Kimberly R&E Ctr, Twin Falls, ID 83341 USA. RP Stark, JC (reprint author), Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. EM jstark@uidaho.edu FU USDA/NIFA FX The authors thank Margaret Bain, Mel Chappell, Lorie Ewing, Nora Fuller, Mark Fristad, Darren Hall, Charlene Miller, Brian Schneider, Lura Schroeder, Steven Wheeler, and Todd Carter, as well as our collaborators in the Western Regional Potato Variety Trials, and the Idaho, Oregon and Washington Potato Commissions for their contributions to the development and release of Mountain Gem Russet. We also express thanks to our industry cooperators for their substantial contributions to this research effort. Development of Mountain Gem Russet was partially funded by USDA/NIFA. NR 10 TC 2 Z9 2 U1 4 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD APR PY 2016 VL 93 IS 2 BP 158 EP 171 DI 10.1007/s12230-015-9493-1 PG 14 WC Agronomy SC Agriculture GA DK5WT UT WOS:000374992600005 ER PT J AU Whitworth, JL Novy, RG Stark, JC Thornton, M Knowles, NR Pavek, MJ Spear, RR Brown, CR Charlton, BA Sathuvalli, V Yilma, S Brandt, TL Olsen, N AF Whitworth, J. L. Novy, R. G. Stark, J. C. Thornton, M. Knowles, N. R. Pavek, M. J. Spear, R. R. Brown, C. R. Charlton, B. A. Sathuvalli, V. Yilma, S. Brandt, T. L. Olsen, N. TI Targhee Russet: A High Yielding, Dual Purpose Potato Variety with High Protein and Vitamin C Content and Resistance to Tuber Soft Rot SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Variety; Breeding; Processing ID LATE BLIGHT; SUGAR ENDS; GERMPLASM; CULTIVAR; DISEASE AB Targhee Russet is a darker-skinned russet potato variety that has shown higher total and marketable yields than Russet Burbank in most yield trials conducted in the western United States. A major strength of Targhee Russet is resistance to tuber soft rot, and it also has moderate resistance to common scab, Verticillium wilt, and net necrosis associated with Potato leafroll virus. Targhee Russet also has exhibited resistance to hollow heart and blackspot bruise. Analyses have shown Targhee Russet to have significantly higher tuber protein and Vitamin C content than Russet Burbank and Ranger Russet. It produces lighter colored fries out of storage than standard processing varieties, indicating its high potential for the production of premium quality frozen and fresh-cut fries. High merit scores for fresh use are also indicative of it having excellent potential as a dual-purpose russet variety, suitable for both processing and fresh-pack operations. C1 [Whitworth, J. L.; Novy, R. G.] USDA ARS, Aberdeen R&E Ctr, Aberdeen, ID 83210 USA. [Stark, J. C.] Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. [Thornton, M.] Univ Idaho, Southwest Idaho R&E Ctr, Parma, ID 83660 USA. [Knowles, N. R.; Pavek, M. J.] Washington State Univ, Pullman, WA 99164 USA. [Spear, R. R.] McCain Foods, Burley, ID 83318 USA. [Brown, C. R.] ARS, USDA, Prosser, WA 99350 USA. [Charlton, B. A.] Oregon State Univ, Klamath Basin R&E Ctr, Klamath Falls, OR 97603 USA. [Sathuvalli, V.; Yilma, S.] Oregon State Univ, Corvallis, OR 97331 USA. [Brandt, T. L.; Olsen, N.] Univ Idaho, Kimberly R&E Ctr, Twin Falls, ID 83341 USA. RP Whitworth, JL (reprint author), USDA ARS, Aberdeen R&E Ctr, Aberdeen, ID 83210 USA. EM jonathan.whitworth@ars.usda.gov FU USDA-NIFA FX The authors thank Margaret Bain, Mel Chappell, Lorie Ewing, Nora Fuller, Mark Fristad, Darren Hall, Chelsey Lowder, Charlene Miller, Brian Schneider, Lura Schroeder, Steven Wheeler, and Todd Carter, as well as our collaborators in the Western Regional Potato Variety Trials, and the Idaho, Oregon and Washington Potato Commissions for their contributions to the development and release of Targhee Russet. We also thank Dr. Rob Davidson for contributions of bacterial ring rot data. We also express thanks to our industry cooperators for their substantial contributions to this research effort. Development of Targhee Russet was partially funded by USDA-NIFA. NR 16 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD APR PY 2016 VL 93 IS 2 BP 189 EP 201 DI 10.1007/s12230-016-9495-7 PG 13 WC Agronomy SC Agriculture GA DK5WT UT WOS:000374992600007 ER PT J AU Miller, PS Lacy, RC Medina-Miranda, R Lopez-Ortiz, R Diaz-Soltero, H AF Miller, Philip S. Lacy, Robert C. Medina-Miranda, Roseanne Lopez-Ortiz, Ricardo Diaz-Soltero, Hilda TI Confronting the invasive species crisis with metamodel analysis: An explicit, two-species demographic assessment of an endangered bird and its brood parasite in Puerto Rico SO BIOLOGICAL CONSERVATION LA English DT Article DE Population viability analysis; Invasive species; Metamodel; Endangered species ID POPULATION VIABILITY ANALYSIS; BLACKBIRD AGELAIUS-XANTHOMUS; MOLOTHRUS-BONARIENSIS; EXTINCTION AB Invasive species pose a significant threat to native species persistence worldwide. Effective invasives management requires a detailed understanding of the mechanisms by which they impact native taxa, yet most quantitative models used to inform management do not address these complex interactions. Populations of the endangered Yellow-shouldered Blackbird (Agelaius xanthomus, YSBL) have declined dramatically across southwestern Puerto Rico, largely through brood parasitism by the invasive Shiny Cowbird (Molothrus bonariensis, SHCO). YSBL management is focused on removing SHCO eggs from Blackbird nests and on constructing artificial nest structures (ANS) that are resistant to terrestrial predators and easily monitored for SHCO parasitism. Despite these efforts, successful recovery of YSBL requires a more thorough knowledge of the complex two-species system. We used a new "metamodel" approach to explicitly simulate demographic interactions between these two species. The metamodel featured two separate, individual-based demographic models running concurrently, with specific data-driven linkages simulating the species interactions and their impacts on population dynamics. Results indicated that YSBL management may be most effective by direct removal of SHCO eggs from parasitized nests, which can also reduce the number of fledged Cowbirds that subsequently prefer YSBL nests as adults. Fledging success and post-fledging survival, previously not considered serious threats, were also identified as critical determinants of YSBL population viability and important targets for management Importantly, trapping of SHCO did not emerge as an effective-method of YSBL management This is among the first population viability simulation models featuring explicit, simultaneous treatment of linked demographic dynamics in a native invasive species system. (C) 2016 The Authors. Published by Elsevier Ltd. C1 [Miller, Philip S.] IUCN SSC Conservat Breeding Specialist Grp, 12101 Johnny Cake Ridge Rd, Apple Valley, MN 55124 USA. [Lacy, Robert C.] Chicago Zool Soc, 3300 Golf Rd, Brookfield, IL 60153 USA. [Medina-Miranda, Roseanne; Lopez-Ortiz, Ricardo] Puerto Rico Dept Nat & Environm Resources, Marina Stn, POB 3665, Mayaguez, PR 00681 USA. [Diaz-Soltero, Hilda] USDA, 1400 Independence Ave SW, Washington, DC 20250 USA. RP Miller, PS (reprint author), IUCN SSC Conservat Breeding Specialist Grp, 12101 Johnny Cake Ridge Rd, Apple Valley, MN 55124 USA. EM pmiller@cbsg.org OI Miller, Philip/0000-0003-3704-7227 FU United States Department of Agriculture (USDA); National Science Foundation (NSF) FX The United States Department of Agriculture (USDA) and The National Science Foundation (NSF) served as sources of financial support for this project Manuscript co-author H. Diaz-Soltero from USDA's Invasives Causing Extinction program participated in the decision to submit this article for publication and helped to write this manuscript. NSF had no direct involvement in this project. NR 38 TC 0 Z9 0 U1 5 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD APR PY 2016 VL 196 BP 124 EP 132 DI 10.1016/j.biocon.2016.01.029 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DL1AW UT WOS:000375365300015 ER PT J AU Scudiero, E Corwin, DL Morari, F Anderson, RG Skaggs, TH AF Scudiero, Elia Corwin, Dennis L. Morari, Francesco Anderson, Ray G. Skaggs, Todd H. TI Spatial interpolation quality assessment for soil sensor transect datasets SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Apparent electrical conductivity; Spatial interpolation; NDVI; On-the-go sensors; R; h-block resampling ID ELECTRICAL-CONDUCTIVITY; CROSS-VALIDATION; AGRICULTURE; VARIABILITY AB Near-ground geophysical soil sensors provide valuable information for precision agriculture applications. Indeed, their readings can be used as proxy for many soil parameters. On-the-go soil sensor surveys are, typically, carried out intensively (e.g., every 2 m) over many parallel transects. Two types of soil sensors measurements are considered in this paper: apparent electrical conductivity (4 fields in California, USA) and reflectance (1 field in Italy). Two types of spatial interpolations are carried out, universal kriging (model-based) and inverse distance weighting (deterministic). Interpolation quality assessment is usually carried out using leave-one-out (loo) resampling. We show that loo resampling on transect sampling datasets returns overly-optimistic, low interpolation errors, because the left-out data point has values very close to that of its neighbors in the training dataset. This bias in the map quality assessment can be reduced by removing the closest neighbors of the validation observation from the training dataset, in a (spatial) h-block (SHB) fashion. The results indicate that, for soil sensor data acquired along parallel transects: (i) the SHB resampling is a useful tool to test the performance of interpolation techniques and (ii) the optimal (i.e., rendering the same errors of un-sampled locations between transects) SHB threshold distance (h.dist) for neighbor-exclusion is proportional to the semi-variogram range and partial sill. This procedure provides research scientists with an improved means of understanding the error of soil maps made by interpolating soil sensor measurements. Published by Elsevier B.V. C1 [Scudiero, Elia; Corwin, Dennis L.; Anderson, Ray G.; Skaggs, Todd H.] USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. [Morari, Francesco] Univ Padua, Dept Agron Food Nat Resources Anim & Environm DAF, Viale Univ 16, I-35020 Legnaro, Italy. RP Scudiero, E (reprint author), USDA ARS, US Salin Lab, 450 West Big Springs Rd, Riverside, CA 92507 USA. EM elia.scudiero@ars.usda.gov OI Scudiero, Elia/0000-0003-4944-721X; Anderson, Ray/0000-0002-6202-5890 FU Office of Naval Research (FMMI) [3200001344]; University of Padua's "GEO-RISKS: Geological, Morphological and Hydrological Processes: Monitoring, Modeling and Impact in the North-Eastern Italy (WP4)" research program FX The funding for this research was from the Office of Naval Research (FMMI number: 3200001344) and the University of Padua's "GEO-RISKS: Geological, Morphological and Hydrological Processes: Monitoring, Modeling and Impact in the North-Eastern Italy (WP4)" research program. The authors wish to acknowledge the hours of diligent technical work that were performed in the field by several technicians whose efforts and conscientiousness were crucial to obtaining the data presented including Michael Bagtang, Wes Clary, and Kevin Yemoto. The authors are especially indebted to the owners of the five selected study sites, for the use of their land, which made the project possible. The authors are thankful to Dr. Kevin Le Rest for his constructive comments on an early version of the manuscript. NR 16 TC 1 Z9 1 U1 3 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD APR PY 2016 VL 123 BP 74 EP 79 DI 10.1016/j.compag.2016.02.016 PG 6 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DK8GY UT WOS:000375166400009 ER PT J AU Yang, C Odvody, GN Thomasson, JA Isakeit, T Nichols, RL AF Yang, Chenghai Odvody, Gary N. Thomasson, J. Alex Isakeit, Thomas Nichols, Robert L. TI Change detection of cotton root rot infection over 10-year intervals using airborne multispectral imagery SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Airborne multispectral imagery; Buffer zone; Change detection; Cotton root rot; Image classification; NDVI ID PHYMATOTRICHUM-OMNIVORUM; IMAGING-SYSTEM; FUNGICIDES AB Cotton root rot is a very serious and destructive disease of cotton grown in the southwestern United States. Accurate information regarding its spatial and temporal distribution within fields is important for effective management of the disease. The objectives of this study were to examine the consistency and variation of cotton root rot infections within cotton fields over 10-year intervals using airborne multispectral imagery and to assess the feasibility to use historical imagery to create prescription maps for site-specific management of the disease. Airborne multispectral images collected from a 102-ha cotton field in 2001 and 2011 and from a 97-ha field in 2002 and 2012 in south Texas were used in this study. The images were rectified and resampled to the same pixel size between the two years for each field. The normalized difference vegetation index (NDVI) images were generated and unsupervised classification was then used to classify the NDVI images into root rot-infected and non-infected zones. Change detection analysis was performed to detect the consistency and change in root rot infection between the two growing seasons for each field. Results indicate that the spatial patterns of the disease were similar between the two seasons, though variations existed for each field. To account for the potential expansion and temporal variation of the disease, buffer zones around the infected areas were created. The buffered maps between the two years agreed well. The results from this study demonstrate that classification maps derived from historical images in conjunction with appropriate buffer zones can be used as prescription maps for site-specific fungicide application to control cotton root rot. Published by Elsevier B.V. C1 [Yang, Chenghai] USDA ARS, Southern Plains Agr Res Ctr, Aerial Applicat Technol Res Unit, 3103 F&B Rd, College Stn, TX 77845 USA. [Odvody, Gary N.] Texas AgriLife Res & Extens Ctr, 10345 State HWY 44, Corpus Christi, TX 78406 USA. [Thomasson, J. Alex] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Isakeit, Thomas] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Nichols, Robert L.] Cotton Inc, 6399 Weston Pkwy, Cary, NC 27513 USA. RP Yang, C (reprint author), USDA ARS, Southern Plains Agr Res Ctr, Aerial Applicat Technol Res Unit, 3103 F&B Rd, College Stn, TX 77845 USA. EM chenghai.yang@ars.usda.gov FU Cotton Incorporated at Cary, North Carolina FX This project was partly funded by Cotton Incorporated at Cary, North Carolina. The authors wish to thank Rene Davis of USDA-ARS at Weslaco, Texas, Lee Denham and Fred Gomez of USDA-ARS at College Station, Texas for taking the imagery for this study. Thanks are also extended to Randy Rachel and John Barrett of Corpus Christi, Texas for allowing us to use their fields. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 21 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 EI 1872-7107 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD APR PY 2016 VL 123 BP 154 EP 162 DI 10.1016/j.compag.2016.02.026 PG 9 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA DK8GY UT WOS:000375166400019 ER PT J AU Coppoletta, M Merriam, KE Collins, BM AF Coppoletta, Michelle Merriam, Kyle E. Collins, Brandon M. TI Post-fire vegetation and fuel development influences fire severity patterns in reburns SO ECOLOGICAL APPLICATIONS LA English DT Article DE fire ecology; fire hazard; fire severity; interacting fires; post-fire restoration; reburn; Sierra Nevada; California; USA ID PONDEROSA PINE FORESTS; MIXED-CONIFER FORESTS; NORTHERN SIERRA-NEVADA; YOSEMITE-NATIONAL-PARK; NORMALIZED BURN RATIO; WESTERN UNITED-STATES; LAKE TAHOE BASIN; SOUTHERN CASCADES; LARGE WILDFIRE; WOODY DEBRIS AB In areas where fire regimes and forest structure have been dramatically altered, there is increasing concern that contemporary fires have the potential to set forests on a positive feedback trajectory with successive reburns, one in which extensive stand-replacing fire could promote more stand-replacing fire. Our study utilized an extensive set of field plots established following four fires that occurred between 2000 and 2010 in the northern Sierra Nevada, California, USA that were subsequently reburned in 2012. The information obtained from these field plots allowed for a unique set of analyses investigating the effect of vegetation, fuels, topography, fire weather, and forest management on reburn severity. We also examined the influence of initial fire severity and time since initial fire on influential predictors of reburn severity. Our results suggest that high-to moderate-severity fire in the initial fires led to an increase in standing snags and shrub vegetation, which in combination with severe fire weather promoted high-severity fire effects in the subsequent reburn. Although fire behavior is largely driven by weather, our study demonstrates that post-fire vegetation composition and structure are also important drivers of reburn severity. In the face of changing climatic regimes and increases in extreme fire weather, these results may provide managers with options to create more fire-resilient ecosystems. In areas where frequent high-severity fire is undesirable, management activities such as thinning, prescribed fire, or managed wildland fire can be used to moderate fire behavior not only prior to initial fires, but also before subsequent reburns. C1 [Coppoletta, Michelle; Merriam, Kyle E.] USDA, US Forest Serv, Sierra Cascade Prov Ecol Program, Quincy, CA 95971 USA. [Collins, Brandon M.] USDA, US Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Collins, Brandon M.] Univ Calif Berkeley, Ctr Fire Res & Outreach, Berkeley, CA 94720 USA. RP Coppoletta, M (reprint author), USDA, US Forest Serv, Sierra Cascade Prov Ecol Program, Quincy, CA 95971 USA. EM mcoppoletta@fs.fed.us FU United States Department of Agriculture Forest Service, Plumas National Forest FX The funding for this study came from United States Department of Agriculture Forest Service, Plumas National Forest. We sincerely thank the following individuals for their support and assistance with data collection: Hugh Safford; T. Gomez and other staff at Natural Resources Management Corporation; E. Jules, M. DeSiervo, T. Harris, L. Negotia, M. Mansfield, and D. Davis from Humboldt State University; W. Cartwright, B. Stewart, S. Causemaker, and M. Friend. J. Baldwin gave very helpful advice on statistical analyses and J. Lydersen, R. Tompkins, and R. Martinez assisted with data processing. NR 89 TC 9 Z9 9 U1 8 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD APR PY 2016 VL 26 IS 3 BP 686 EP 699 DI 10.1890/15-0225 PG 14 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DK3TO UT WOS:000374840500004 PM 27411243 ER PT J AU Buhnerkempe, MG Webb, CT Merton, AA Buhnerkempe, JE Givens, GH Miller, RS Hoeting, JA AF Buhnerkempe, Michael G. Webb, Colleen T. Merton, Andrew A. Buhnerkempe, John E. Givens, Geof H. Miller, Ryan S. Hoeting, Jennifer A. TI Identification of migratory bird flyways in North America using community detection on biological networks SO ECOLOGICAL APPLICATIONS LA English DT Article DE Anas acuta; Anas carolinensis; Anas platyrhnchos; Branta canadensis; community detection; consolidation factor; flyways; migration; network; waterfowl ID ADAPTIVE MANAGEMENT; WATERFOWL HARVESTS; HUMAN INFECTION; INFLUENZA; ORIGIN; VIRUS; UNCERTAINTY; MOVEMENTS; HABITAT; PRAIRIE AB Migratory behavior of waterfowl populations in North America has traditionally been broadly characterized by four north-south flyways, and these flyways have been central to the management of waterfowl populations for more than 80 yr. However, previous flyway characterizations are not easily updated with current bird movement data and fail to provide assessments of the importance of specific geographical regions to the identification of flyways. Here, we developed a network model of migratory movement for four waterfowl species, Mallard (Anas platyrhnchos), Northern Pintail (A. acuta), American Green-winged Teal (A. carolinensis), and Canada Goose (Branta canadensis), in North America, using bird band and recovery data. We then identified migratory flyways using a community detection algorithm and characterized the importance of smaller geographic regions in identifying flyways using a novel metric, the consolidation factor. We identified four main flyways for Mallards, Northern Pintails, and American Green-winged Teal, with the flyway identification in Canada Geese exhibiting higher complexity. For Mallards, flyways were relatively consistent through time. However, consolidation factors revealed that for Mallards and Green-winged Teal, the presumptive Mississippi flyway was potentially a zone of high mixing between other flyways. Our results demonstrate that the network approach provides a robust method for flyway identification that is widely applicable given the relatively minimal data requirements and is easily updated with future movement data to reflect changes in flyway definitions and management goals. C1 [Buhnerkempe, Michael G.; Merton, Andrew A.; Givens, Geof H.; Hoeting, Jennifer A.] Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA. [Buhnerkempe, Michael G.; Webb, Colleen T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Buhnerkempe, Michael G.] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA. [Buhnerkempe, Michael G.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. [Buhnerkempe, John E.] Illinois Dept Nat Resources, Div Wildlife Resources, Springfield, IL 62702 USA. [Miller, Ryan S.] USDA, Anim & Plant Hlth Inspect Serv, Ctr Epidemiol, Ft Collins, CO 80521 USA. [Miller, Ryan S.] USDA, Anim & Plant Hlth Inspect Serv, Ctr Anim Hlth, Ft Collins, CO 80521 USA. [Givens, Geof H.] Givens Stat Solut LLC, Ft Collins, CO 80526 USA. RP Buhnerkempe, MG (reprint author), Colorado State Univ, Dept Stat, Ft Collins, CO 80523 USA.; Buhnerkempe, MG (reprint author), Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA.; Buhnerkempe, MG (reprint author), Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA.; Buhnerkempe, MG (reprint author), NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. EM michael.buhnerkempe@gmail.com OI Miller, Ryan/0000-0003-3892-0251 FU Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate, Department of Homeland Security; Fogarty International Center, National Institutes of Health; USDA [07-7100-0228-CA9, 09-7100-0305-CA, 11-9208-0269-CA, 09-9208-0235-CA] FX M. G. Buhnerkempe and C. T. Webb acknowledge funding from the Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate, Department of Homeland Security, and Fogarty International Center, National Institutes of Health. Funding for this work was also provided by USDA Cooperative Agreements 07-7100-0228-CA9, 09-7100-0305-CA, 11-9208-0269-CA and 09-9208-0235-CA. We would like to thank Shweta Bansal, Dan Grear, and Clint Leach for useful discussions on conceptual and methodological issues related to network analyses. NR 41 TC 2 Z9 2 U1 9 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD APR PY 2016 VL 26 IS 3 BP 740 EP 751 DI 10.1890/15-0934 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DK3TO UT WOS:000374840500008 PM 27411247 ER PT J AU Xie, R Tu, MB Elder, T AF Xie, Rui Tu, Maobing Elder, Thomas TI Substituent Effect of Phenolic Aldehyde Inhibition on Alcoholic Fermentation by Saccharomyces cerevisiae SO ENERGY & FUELS LA English DT Article ID ELECTROPHILIC REACTIVITY; HEPATOCYTE TOXICITY; AROMATIC-COMPOUNDS; LIGNOCELLULOSIC BIOMASS; TETRAHYMENA-PYRIFORMIS; ZYMOMONAS-MOBILIS; ESCHERICHIA-COLI; MODEL COMPOUNDS; DETOXIFICATION; PRETREATMENT AB Phenolic compounds significantly inhibit microbial fermentation of biomass hydrolysates. To understand the quantitative structure inhibition relationship of phenolic aldehydes on alcoholic fermentation, the effect of 11 different substituted benzaldehydes on the final ethanol yield was examined. The results showed that the degree of phenolic benzaldehyde inhibition was strongly associated with the position of phenolic hydroxyl groups but not the number of phenolic hydroxyl groups. It was observed that ortho-substituted 2-hydroxybenzaldehyde resulted in 15-20-fold higher inhibition than the meta- or para-substituted analogues of 3- and 4-hydroxybenzaldehydes. From the correlation of the molecular descriptors to inhibition potency in yeast fermentation, we found a strong relationship between the octanol/water partition coefficient (log P) of aldehydes and the EC50 value. The most inhibitory 2-hydroxybenzaldehyde has the highest log P and possesses an ortho OH group capable of forming an intramolecular hydrogen bond, which can potentially increase the cell membrane permeability and toxicity. The results also indicated that the calculated free energy change between phenolic aldehydes and amino acids can be used to predict their structure inhibitory activity relationship. C1 [Xie, Rui] Auburn Univ, Forest Prod Lab, 520 Devall Dr, Auburn, AL 36849 USA. [Xie, Rui] Auburn Univ, Ctr Bioenergy & Bioprod, 520 Devall Dr, Auburn, AL 36849 USA. [Tu, Maobing] Univ Cincinnati, Dept Biomed Chem & Environm Engn, 2901 Woodside Dr, Cincinnati, OH 45221 USA. [Elder, Thomas] USDA Forest Serv, Southern Res Stn, 521 Devall Dr, Auburn, AL 36849 USA. RP Tu, MB (reprint author), Univ Cincinnati, Dept Biomed Chem & Environm Engn, 2901 Woodside Dr, Cincinnati, OH 45221 USA. EM tumg@uc.edu FU United States Department of Energy [DE-EE0003115]; National Science Foundation [NSF-CBET 1555633]; Alabama Supercomputer Authority FX The authors appreciate the support of this study, partially, by grants from the United States Department of Energy (DE-EE0003115) and the National Science Foundation (NSF-CBET 1555633). This work was made possible in part by a grant of high-performance computing resources and technical support from the Alabama Supercomputer Authority. NR 30 TC 2 Z9 2 U1 3 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD APR PY 2016 VL 30 IS 4 BP 3078 EP 3084 DI 10.1021/acs.energyfuels.5b03034 PG 7 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DK3GF UT WOS:000374804400059 ER PT J AU Feng, WY Zhu, YR Wu, FC Meng, W Giesy, J He, ZQ Song, LR Fan, M AF Feng, Weiying Zhu, Yuanrong Wu, Fengchang Meng, Wei Giesy, John P. He, Zhongqi Song, Lirong Fan, Mingle TI Characterization of phosphorus forms in lake macrophytes and algae by solution P-31 nuclear magnetic resonance spectroscopy SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Aquatic macrophytes; Algae; Organic phosphorus; P-31-NMR; Lake; Orthophosphate monoesters ID ORGANIC PHOSPHORUS; NMR-SPECTROSCOPY; ENZYMATIC-HYDROLYSIS; BIOGENIC PHOSPHORUS; FRESH-WATER; SEDIMENTS; SOIL; CHINA; BIOAVAILABILITY; FRACTIONATION AB Debris from aquatic macrophytes and algae are important recycling sources of phosphorus (P), which can result in continuing blooms of algae by recycling bioavailable P in the eutrophic lakes. However, knowledge of forms of P in aquatic macrophytes and algae and their contribution to internal loads of P in lakes is limited. Without such knowledge, it is difficult to develop appropriate strategies to remediate and or restore aquatic ecosystems that have become eutrophic. Therefore, in this work, P was extracted from six types of aquatic macrophytes and algae collected from Tai Lake of China and characterized by use of solution P-31-nuclear magnetic resonance (NMR) spectroscopy. When extracted by 0.5 M NaOH-25 mM EDTA, extraction recovery of total P(TP) and organic P(P-o) exceeded 90 %. Concentrations of P-o in algae and aquatic macrophytes were 5552 mg kg(-1) and 1005 mg kg(-1) and accounted for 56.0 and 47.2 % of TP, respectively. When P-o, including condensed P, was characterized by solution P-31-NMR P-o in algae included orthophosphate monoesters (79.8 %), pyrophosphate (18.2 %), and orthophosphate diester (2.0 %), and P-o in aquatic macrophytes included orthophosphate monoesters (90.3 %), pyrophosphate (4.2 %), and orthophosphate diester (5.5 %). Additionally, orthophosphate monoesters in algal debris mainly included beta-glycerophosphate (44.1 %), alpha-glycerophosphate (13.5 %), and glucose 6-phosphate (13.5 %). Orthophosphate monoesters in aquatic macrophytes mainly included beta-glycerophosphate (27.9 %), alpha-glycerophosphate (24.6 %), and adenosine 5' monophosphate (8.2 %). Results derived from this study will be useful in better understanding nutrient cycling, relevant eutrophication processes, and pollution control for freshwater lakes. C1 [Feng, Weiying] Beijing Normal Univ, Coll Water Sci, Beijing 100875, Peoples R China. [Feng, Weiying; Zhu, Yuanrong; Wu, Fengchang; Meng, Wei; Giesy, John P.] Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. [Giesy, John P.] Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada. [Giesy, John P.] Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada. [Giesy, John P.] Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China. [He, Zhongqi] USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. [Song, Lirong] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. [Fan, Mingle] Procter & Gamble Co, Cincinnati, OH 45252 USA. RP Zhu, YR; Wu, FC (reprint author), Chinese Res Inst Environm Sci, State Key Lab Environm Criteria & Risk Assessment, Beijing 100012, Peoples R China. EM zhuyuanrong07@mails.ucas.ac.cn; wufengchang@vip.skleg.cn FU National Natural Science Foundation of China [41403094, 41403743, 41261140337]; Canada Research Chair program; Einstein Professor Program of the Chinese Academy of Sciences; State Administration of Foreign Experts Affairs, the P. R. China [GDT20143200016] FX This present study was supported in part by the National Natural Science Foundation of China (41403094, 41403743, 41261140337). Prof. Giesy was supported by the Canada Research Chair program, the Einstein Professor Program of the Chinese Academy of Sciences, and the Great-Tier Foreign Scholar program (#GDT20143200016), funded by the State Administration of Foreign Experts Affairs, the P. R. China to Nanjing University. NR 45 TC 2 Z9 2 U1 8 U2 19 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD APR PY 2016 VL 23 IS 8 BP 7288 EP 7297 DI 10.1007/s11356-015-5913-5 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK5XN UT WOS:000374994600025 PM 26681323 ER PT J AU Giunta, AD Jenkins, MJ Hebertson, EG Munson, AS AF Giunta, Andrew D. Jenkins, Michael J. Hebertson, Elizabeth G. Munson, Allen S. TI Disturbance Agents and Their Associated Effects on the Health of Interior Douglas-Fir Forests in the Central Rocky Mountains SO FORESTS LA English DT Review DE interior Douglas-fir forest; Douglas-fir beetle; western spruce budworm; disturbance; forest health; mixed-severity fire ID WESTERN SPRUCE BUDWORM; BARK BEETLE OUTBREAKS; COLORADO FRONT RANGE; DENDROCTONUS-PSEUDOTSUGAE HOPKINS; TREE-RING RECONSTRUCTION; SANTA CATALINA MOUNTAINS; PONDEROSA PINE FOREST; MIXED-CONIFER FOREST; SUB-ALPINE FORESTS; UNITED-STATES AB Interior Douglas-fir is a prevalent forest type throughout the central Rocky Mountains. Past management actions, specifically fire suppression, have led to an expansion of this forest type. Although Douglas-fir forests cover a broad geographic range, few studies have described the interactive effects of various disturbance agents on forest health conditions. In this paper, we review pertinent literature describing the roles, linkages, and mechanisms by which disturbances, including insect outbreaks, pathogens, fire, and other abiotic factors, affect the development, structure, and distribution of interior montane forests primarily comprised of Douglas-fir. We also discuss how these effects may influence important resource values such as water, biodiversity, wildlife habitat, timber, and recreation. Finally, we identify gaps where further research may increase our understanding of these disturbance agents, their interacting roles, and how they influence long-term forest health. C1 [Giunta, Andrew D.; Jenkins, Michael J.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Hebertson, Elizabeth G.; Munson, Allen S.] USDA, US Forest Serv, Forest Hlth Protect, Ogden, UT 84403 USA. RP Giunta, AD (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM adgiunta@gmail.com; mike.jenkins@usu.edu; lghebertson@fs.fed.us; smunson@fs.fed.us FU Joint Fire Science Program [11-1-4-16]; Ecology Center; Utah Agricultural Experiment Station at Utah State University FX Funding for this project was provided by the Joint Fire Science Program (Project #11-1-4-16) and supported by the Ecology Center and the Utah Agricultural Experiment Station at Utah State University. We give special thanks to Michaela Teich for assistance with manuscript preparation and review, Wanda Lindquist for graphics assistance, and Laura Dunning for manuscript formatting. The comments from four anonymous reviewers have greatly improved the final manuscript. NR 251 TC 0 Z9 0 U1 12 U2 20 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD APR PY 2016 VL 7 IS 4 AR 80 DI 10.3390/f7040080 PG 30 WC Forestry SC Forestry GA DK8WY UT WOS:000375210700008 ER PT J AU Keasberry, AM Hart, JL Dey, DC Schweitzer, CJ AF Keasberry, Amanda M. Hart, Justin L. Dey, Daniel C. Schweitzer, Callie J. TI Spatial Patterns of Irradiance and Advanced Reproduction along a Canopy Disturbance Severity Gradient in an Upland Hardwood Stand SO FORESTS LA English DT Article DE competition; oak (Quercus); photosynthetically active radiation (PAR); wind disturbance ID EASTERN NORTH-AMERICA; SCALE WIND DISTURBANCE; CUMBERLAND PLATEAU; OAK FORESTS; RED MAPLE; QUERCUS STANDS; UNITED-STATES; ACCELERATED SUCCESSION; NATURAL REGENERATION; COMPETITION INDEXES AB Regeneration failure of Quercus in mature Quercus-dominated forests has been reported throughout the temperate zone. Quercus seedlings are often abundant in these forests, yet frequently fail to recruit to larger size classes despite canopy disturbances. To examine intra-stand patterns of advanced reproduction, competition, and irradiance in an upland Quercus stand, we installed a 2 ha plot that captured the canopy disturbance severity gradient caused by a wind event. To quantify disturbance severity and stand conditions, we inventoried all living and dead woody stems >= 5 cm diameter at breast height (dbh, 1.37 m above the surface) and quantified irradiance in 25 m(2) quadrats (n = 800) using synchronized ceptometers. To inventory patterns of advanced reproduction within the plot, we recorded the species of every woody stem >1 m in height and <5 cm dbh. We also documented the species and height of every Quercus stem >= 0.5-<5 cm dbh and the species, height, and distance to each stem nearest the focal Quercus stem. At the genus-level, Quercus was the most common nearest neighbor. However, at the species-level, the most common nearest neighbors were Acer saccharum and Ostrya virginiana. Competition index values significantly differed by species (p < 0.01), but did not significantly differ by disturbance severity class and we found no significant interactions between species and disturbance class. Quercus advanced reproduction was significantly clustered through the study plot and cluster locations overlapped with clusters of high irradiance, but these patterns were scale-dependent. Our results indicate that an appreciation for intra-stand heterogeneity may improve forest management planning. C1 [Keasberry, Amanda M.; Hart, Justin L.] Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. [Dey, Daniel C.] USDA, US Forest Serv, No Res Stn, Columbia, MO 65211 USA. [Schweitzer, Callie J.] USDA, US Forest Serv, Southern Res Stn, Huntsville, AL 35801 USA. RP Hart, JL (reprint author), Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. EM amkeasberry@crimson.ua.edu; hart013@ua.edu RI Hart, Justin/B-2535-2010; OI Hart, Justin/0000-0001-6580-0189; Dey, Daniel/0000-0003-4762-7452 FU Northern Research Station; USDA Forest Service; University of Alabama FX This research was funded as a Joint Venture Agreement between the Northern Research Station, USDA Forest Service and the University of Alabama. We thank Win Cowden, Lauren Cox, and Cindy Taylor for assistance in the field, the Bankhead National Forest staff for logistical support, and Michael Steinberg and Lauren Cox for comments on prior drafts of the manuscript. NR 84 TC 2 Z9 2 U1 3 U2 4 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD APR PY 2016 VL 7 IS 4 AR 73 DI 10.3390/f7040073 PG 18 WC Forestry SC Forestry GA DK8WY UT WOS:000375210700001 ER PT J AU Chen, H McCollum, G Baldwin, E Bail, J AF Chen, Hong McCollum, Greg Baldwin, Elizabeth Bail, Jinhe TI Impacts of Huanglongbing Symptom Severity on Fruit Detachment Force and Mechanical Properties of Sweet Oranges (Citrus sinensis) SO HORTSCIENCE LA English DT Article DE abscission; fruit drop; peel toughness; fruit firmness; puncture damage ID TEXTURE PROFILE ANALYSIS; ABSCISSION; DISEASE; VARIETIES; PEEL; TPA AB This study evaluated the effects of huanglongbing (HLB) symptom severity on fruit detachment force (FDF) and fruit mechanical properties in sweet oranges (Citrus sinensis) as indicators of potential HLB-influenced preharvest fruit drop and postharvest damage and breakdown. FDF for fruit from HLB-asymptomatic trees (AS) was substantially greater than for fruits from HLB-symptomatic trees or branches. Fruit hardness, resilience, springiness, and cohesiveness values, measured by texture profile analysis (TPA), represent various aspects of fruit firmness and recovery capacity once compressed, and were lower in HLB-symptomatic fruits in comparison with AS fruits. On the other hand, puncture force, toughness, and deformation values, measured by a puncture test and representing peel toughness, were higher in HLB-symptomatic fruits than in the AS fruits. The results indicate that fruits from severely HLB-symptomatic sweet orange trees are more likely to have problems with preharvest fruit drop, and postharvest pressure damage and breakdown, but may have less puncture damage in harvesting, transportation, packing, and juice processing. C1 [Chen, Hong] Huazhong Agr Univ, Coll Engn, Wuhan 430070, Hubei, Peoples R China. [Chen, Hong; McCollum, Greg; Baldwin, Elizabeth; Bail, Jinhe] ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP Bail, J (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov FU China Scholarship Council [201206765008]; Fundamental Research Funds for the Central Universities of China [2013PY127] FX We thank the China Scholarship Council (201206765008) and Fundamental Research Funds for the Central Universities of China (2013PY127) for their financial support for this research. NR 42 TC 1 Z9 1 U1 3 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD APR PY 2016 VL 51 IS 4 BP 356 EP 361 PG 6 WC Horticulture SC Agriculture GA DL0ZN UT WOS:000375361600008 ER PT J AU Zambrana-Echevarria, C De Jesus-Kim, L Marquez-Karry, R Siritunga, D Jenidne, D AF Zambrana-Echevarria, Cristina De Jesus-Kim, Lorriane Marquez-Karry, Rocio Siritunga, Dimuth Jenidne, David TI Diversity of Papaya ringspot virus Isolates in Puerto Rico SO HORTSCIENCE LA English DT Article DE Carica papaya; Papaya ringspot virus; PRSV; coat protein gene; Puerto Rico; diversity ID COAT PROTEIN GENE; WATERMELON MOSAIC VIRUS-1; MOMORDICA-CHARANTIA; MULTIPLE LOCATIONS; MOLECULAR ANALYSIS; TRANSGENIC PAPAYA; POTYVIRUS; JAMAICA; HOST; PATHOGENICITY AB Papaya ringspot virus (PRSV) devastates papaya production worldwide. In Puerto Rico, papaya fields can be completely infected with PRSV within a year of planting. Information about the diversity of the Puerto Rican PRSV (PR-PRSV) population is relevant to establish a control strategy in the island. The coat protein gene (cp) of PRSV was sequenced from 62 isolates from different regions in Puerto Rico. The viral population of PRSV in Puerto Rico has 4% nucleotide and 5% amino acid diversity. Analysis of the coat protein (CP) amino acid sequence showed a variable amino terminal (N-terminal) region with a conserved aphid transmission motif and a variable EK repeat region. The core and carboxyl terminal (C-terminal) region were conserved. In the phylogenetic analysis, Puerto Rican isolates grouped independently of their geographical origin, with the exception of southern isolates that formed two separate subgroups and were the most divergent. Sequences of the cp from the Puerto Rican isolates, when compared with sequences from other countries, showed least genetic distance with isolates from the United States and Australia, followed by other American and Caribbean isolates. The U.S. and Australian isolates are sister taxa to the Puerto Rican isolates in the phylogenetic tree. This suggests that PRSV from Puerto Rico and the isolates from the United States and Australia have a common origin thought to be from a Mexican population. C1 [Zambrana-Echevarria, Cristina; De Jesus-Kim, Lorriane; Marquez-Karry, Rocio; Siritunga, Dimuth] Univ Puerto Rico, Mayaguez, PR 00681 USA. [Jenidne, David] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00681 USA. [Jenidne, David] South Carolina Forestry Commiss, Columbia, SC 29212 USA. RP Siritunga, D (reprint author), Univ Puerto Rico, Mayaguez, PR 00681 USA. EM dimuth.siritunga@upr.edu FU Howard Hughes Medical Institute grant [52007566]; U.S. Department of Agriculture-Tropical Agriculture Research Station (Mayaguez, Puerto Rico) FX This study was funded by Howard Hughes Medical Institute grant to DS (award no. 52007566).; We thank Robert McPhail for helping in the collection of materials and serological testing and the U.S. Department of Agriculture-Tropical Agriculture Research Station (Mayaguez, Puerto Rico) for partial funding and for the use of facilities. NR 54 TC 0 Z9 0 U1 1 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD APR PY 2016 VL 51 IS 4 BP 362 EP 369 PG 8 WC Horticulture SC Agriculture GA DL0ZN UT WOS:000375361600009 ER PT J AU Sawaengsri, H Wang, JP Reginaldo, C Steluti, J Wu, DY Meydani, SN Seihub, J Paul, L AF Sawaengsri, Hathairat Wang, Junpeng Reginaldo, Christina Steluti, Josiane Wu, Dayong Meydani, Simin Nikbin Seihub, Jacob Paul, Ligi TI High folic acid intake reduces natural killer cell cytotoxicity in aged mice SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Folic acid; Folate; Natural killer cell cytotoxicity; immune function; Interleukin-10 ID RANDOMIZED CONTROLLED-TRIAL; FOLATE CONCENTRATIONS; NK-CELLS; DIHYDROFOLATE-REDUCTASE; CARDIOVASCULAR-DISEASE; GENERAL-POPULATION; CANCER INCIDENCE; FLOW-CYTOMETRY; BREAST-CANCER; T-CELLS AB Presence of unmetabolized folic acid in plasma, which is indicative of folic acid intake beyond the metabolic capacity of the body, is associated with reduced natural killer (NK) cell cytotoxicity in postmenopausal women years. NK cells are cytotoxic lymphocytes that are part of the innate immune system critical for surveillance and defense against virus-infected and cancer cells. We determined if a high folic acid diet can result in reduced NK cell cytotoxicity in an aged mouse model. Female C57BL/6 mice (16-month-old) were fed an AIN-93 M diet with the recommended daily allowance (1 x RDA, control) or 20 x RDA (high) folic acid for 3 months. NK cytotoxicity was lower in splenocytes from mice fed a high folic acid diet when compared to mice on control diet (P<.04). The lower NK cell cytotoxicity in high folic acid fed mice could be due to their lower mature cytotoxic/naive NK cell ratio (P=.03) when compared to the control mice. Splenocytes from mice on high folic acid diet produced less interleukin (IL)-10 when stimulated with lipopolysaccharide (P<.05). The difference in NK cell cytotoxicity between dietary groups was abolished when the splenocytes were supplemented with exogenous IL-10 prior to assessment of the NK cytotoxicity, suggesting that the reduced NK cell cytotoxicity of the high folic acid group was at least partially due to. reduced IL-10 production. This study demonstrates a causal relationship between high folic acid intake and reduced NK cell cytotoxicity and provides some insights into the potential mechanisms behind this relationship. (C) 2016 Elsevier Inc. All rights reserved. C1 [Sawaengsri, Hathairat; Wang, Junpeng; Reginaldo, Christina; Steluti, Josiane; Wu, Dayong; Meydani, Simin Nikbin; Seihub, Jacob; Paul, Ligi] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [Wang, Junpeng] Henan Univ, Huaihe Clin Coll, Translat Med Ctr, Kaifeng 475000, Peoples R China. [Steluti, Josiane] Univ Sao Paulo, Dept Nutr, Sch Publ Hlth, BR-01246904 Sao Paulo, Brazil. RP Paul, L (reprint author), Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Ligi.Paul_Pottenplackel@tufts.edu FU United States Department of Agriculture [58-1950-0-014]; Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University FX We thank Dr. Donald Smith for his assistance with animal care and maintenance and Mr. Alex Histed for his assistance with the NK cell cytotoxicity assays. Support from United States Department of Agriculture Specific Cooperative Agreement #58-1950-0-014 and pilot grant from Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University. Any opinions, findings, conclusion or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the United States Department of Agriculture. NR 45 TC 4 Z9 4 U1 4 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD APR PY 2016 VL 30 BP 102 EP 107 DI 10.1016/j.jnutbio.2015.12.006 PG 6 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DJ6TY UT WOS:000374347300011 PM 27012626 ER PT J AU Croat, JR Berhow, M Karki, B Muthukumarappan, K Gibbons, WR AF Croat, Jason R. Berhow, Mark Karki, Bishnu Muthukumarappan, Kasiviswanathan Gibbons, William R. TI Conversion of Canola Meal into a High-Protein Feed Additive via Solid-State Fungal Incubation Process SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Canola; Glucosinolates; Rapeseed; Solid-state incubation ID FERMENTATION; RAPESEED; LIQUID; PH; METABOLISM; CHALLENGES; MYROSINASE; PULLULAN; NITROGEN; DEFENSE AB The study goal was to determine the optimal fungal culture to reduce glucosinolates (GLS), fiber, and residual sugars while increasing the protein content and nutritional value of canola meal. Solid-state incubation conditions were used to enhance filamentous growth of the fungi. Flask trials were performed using 50 % moisture content hexane-extracted (HE) or cold-pressed (CP) canola meal with incubation for 168 h at 30 A degrees C. Incubation on HE canola meal Trichoderma reesei (NRRL-3653) achieved the greatest increase in protein content (23 %), while having the lowest residual levels of sugar (8 % w/w) and GLS (0.4 mu M/g). Incubation on CP canola meal Trichoderma reesei (NRRL-3653), A. pullulans (NRRL-58522), and A. pullulans (NRRL-Y-2311-1) resulted in the greatest improvement in protein content (22.9, 16.9 and 15.4 %, respectively), while reducing total GLS content from 60.6 to 1.0, 3.2 and 10.7 mu M/g, respectively. HE and CP canola meal GLS levels were reduced to 65.5 and 50.7 % by thermal treatments while solid-state microbial conversion further reduced GLS up to 99 and 98 %, respectively. Fiber levels increased due to the concentration effect of removing oligosaccharides and GLS. C1 [Croat, Jason R.; Gibbons, William R.] S Dakota State Univ, Biol & Microbiol Dept, 228 Alfred Dairy Sci Hall, Brookings, SD 57007 USA. [Berhow, Mark] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Karki, Bishnu; Muthukumarappan, Kasiviswanathan] S Dakota State Univ, Dept Agr & Biosyst Engn, Brookings, SD 57007 USA. RP Croat, JR (reprint author), S Dakota State Univ, Biol & Microbiol Dept, 228 Alfred Dairy Sci Hall, Brookings, SD 57007 USA. EM jason.croat@sdstate.edu OI Croat, Jason/0000-0002-0810-5152 FU South Dakota Oilseed Initiative; South Dakota Oilseed Commission FX This work was performed in the Alfred Dairy Science Hall and was supported by the South Dakota Oilseed Initiative and a grant from the South Dakota Oilseed Commission. We would like to thank Kerry O'Donnell and the Bacterial Foodborne Pathogens and Mycology Research Unit of the United States Department of Agriculture for identifying the M. circincelloides and P. kudriavzevii strains used in this research. We would also like to thank Ray Holloway, Vanessa Voelker, and Sandra M. Duval for their technical assistance in the GLS analyses. NR 48 TC 0 Z9 0 U1 10 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD APR PY 2016 VL 93 IS 4 BP 499 EP 507 DI 10.1007/s11746-016-2796-7 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DJ7QJ UT WOS:000374405600005 ER PT J AU Moreau, RA Harron, AF Powell, MJ Hoyt, JL AF Moreau, Robert A. Harron, Andrew F. Powell, Michael J. Hoyt, Jonathan L. TI A Comparison of the Levels of Oil, Carotenoids, and Lipolytic Enzyme Activities in Modern Lines and Hybrids of Grain Sorghum SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Sorghum; Corn; Carotenoids; Lutein; Lipolysis; Lipase ID FLOUR; CORN AB Carotenoids are potentially valuable components in grain sorghum and there is a need to better understand their concentration, composition, and value. Thirteen modern commercial grain sorghum hybrids and five sorghum lines were extracted and analyzed and the levels of oil and carotenoids were compared. The same samples were also evaluated for lipolytic enzyme activity. The oil content in all eighteen samples ranged from 3.21 to 4.29 wt%. Lutein and zeaxanthin were the predominant carotenoids and the levels of total carotenoids ranged from 3.82 to 19.5 ppm in the oil, which was much lower than the levels of total carotenoids in two yellow corn samples (70.8 and 103 ppm). Lipolytic enzyme activity was estimated by storing milled kernel samples for 2 weeks at 25 A degrees C and measuring the levels of total free fatty acids. After 2 weeks, the levels of free fatty acids in the oil of the eighteen sorghum samples ranged from 11.49 and 52.17 wt%, compared to 10.29 and 17.54 wt% in oil from the two corn samples. This new data will be useful for persons using grain sorghum for food, biofuels and other industrial applications. Examples of sorghum genotypes with high and low levels of carotenoids and high and low levels of lipolytic enzymes were both identified. C1 [Moreau, Robert A.; Harron, Andrew F.; Powell, Michael J.; Hoyt, Jonathan L.] ARS, Sustainable Biofuels & CoProd Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Moreau, RA (reprint author), ARS, Sustainable Biofuels & CoProd Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM robert.moreau@ars.usda.gov OI Moreau, Robert/0000-0002-8166-8322 FU United Sorghum Checkoff Program FX We would like to thank: The United Sorghum Checkoff Program for providing finanacial support, Dr. Bill Rooney, at Texas A&M for providing four samples of sorghum breeding lines of sorghum, Dr. Scott Bean at USDA, ARS, for providing samples of sorghum cultivar Macia, Mr. Dan Berning from at DuPont Pioneer for providing 13 samples of modern sorghum hybrids, and Dr. Bryan Vinyard, USDA, ARS, for valuable assistance with statistical analyses. NR 11 TC 1 Z9 1 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD APR PY 2016 VL 93 IS 4 BP 569 EP 573 DI 10.1007/s11746-016-2799-4 PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DJ7QJ UT WOS:000374405600011 ER PT J AU Work, TM Verma, SK Su, CL Medeiros, J Kaiakapu, T Kwok, OC Dubey, JP AF Work, Thierry M. Verma, Shiv K. Su, Chunlei Medeiros, John Kaiakapu, Thomas Kwok, Oliver C. Dubey, Jitender P. TI TOXOPLASMA GONDII ANTIBODY PREVALENCE AND TWO NEW GENOTYPES OF THE PARASITE IN ENDANGERED HAWAIIAN GEESE (NENE: BRANTA SANDVICENSIS) SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Birds; cats; genotype; island; PCR-RFLP genotyping; strain ID UNITED-STATES; DISEASE; CATS; SEROPREVALENCE; LINEAGES; PATTERNS; WILDLIFE; ECOLOGY; OOCYSTS; BIRDS AB Toxoplasma gondii is a protozoan parasite transmitted by domestic cats (Felis catus) that has historically caused mortality in native Hawaiian birds. To estimate how widespread exposure to the parasite is in nene (Hawaiian Geese, Branta sandvicensis), we did a serologic survey for T. gondii antibody and genetically characterized parasite DNA from the tissues of dead birds that had confirmed infections by immunohistochemistry. Of 94 geese sampled, prevalence on the island of Kauai, Maui, and Molokai was 21% (n=42), 23% (n=31), and 48% (n=21), respectively. Two new T. gondii genotypes (ToxoDB #261 and #262) were identified by PCR-restriction fragment length polymorphism from four geese, and these appeared segregated geographically. Exposure to T. gondii in wild nene is widespread and, while the parasite is not a major cause of death, it could have sublethal or behavioral effects. How to translate such information to implement effective ways to manage feral cats in Hawaii poses challenges. C1 [Work, Thierry M.] US Geol Survey, Natl Wildlife Hlth Ctr, Honolulu Field Stn, POB 50187, Honolulu, HI 96850 USA. [Verma, Shiv K.; Kwok, Oliver C.; Dubey, Jitender P.] ARS, USDA, Anim Parasit Dis Lab, 10300 Baltimore Ave,Bldg 1001 BARC East, Beltsville, MD 20705 USA. [Su, Chunlei] Univ Tennessee, Dept Microbiol, Coll Arts & Sci, M409 Walters Life Sci, Knoxville, TN 37996 USA. [Medeiros, John] State Hawaii, Div Forestry & Wildlife Maui, 54 S High St 101, Wailuku, HI 96793 USA. [Kaiakapu, Thomas] State Hawaii, Div Forestry & Wildlife Kauai, 3060 Eiwa St 306, Lihue, HI 96766 USA. RP Work, TM (reprint author), US Geol Survey, Natl Wildlife Hlth Ctr, Honolulu Field Stn, POB 50187, Honolulu, HI 96850 USA. EM thierry_work@usgs.gov NR 33 TC 0 Z9 0 U1 11 U2 17 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD APR PY 2016 VL 52 IS 2 BP 253 EP 257 DI 10.7589/2015-09-235 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DK8XP UT WOS:000375213100007 PM 26967138 ER PT J AU Graham, ER Sanders, RW AF Graham, Erin R. Sanders, Robert W. TI Species-specific photosynthetic responses of symbiotic zoanthids to thermal stress and ocean acidification SO MARINE ECOLOGY-AN EVOLUTIONARY PERSPECTIVE LA English DT Article DE Climate change; cnidarian; ocean acidification; Symbiodinium; zoanthid ID CO2 PARTIAL-PRESSURE; ANEMONE ANTHOPLEURA-ELEGANTISSIMA; CORAL-DINOFLAGELLATE SYMBIOSIS; REEF-BUILDING CORALS; NATURAL PH GRADIENT; GREAT-BARRIER-REEF; SCLERACTINIAN CORAL; CLIMATE-CHANGE; HEAT-STRESS; SYMBIODINIUM DINOPHYCEAE AB Increasing sea-surface temperatures and ocean acidification (OA) are impacting physiologic processes in a variety of marine organisms. Many sea anemones, corals and jellies in the phylum Cnidaria form endosymbiotic relationships with Symbiodinium spp. (phylum Dinoflagellata) supply the hosts with fixed carbon from photosynthesis. Much work has focused on the generally negative effects of rising temperature and OA on calcification in Symbiodinium-coral symbioses, but has not directly measured symbiont photosynthesis in hospite or fixed carbon translocation from symbiont to host. Symbiodinium species or types vary in their environmental tolerance and photosynthetic capacity; therefore, primary production in symbiotic associations can vary with symbiont type. However, symbiont type has not been identified in a large portion of Symbiodinium-cnidarian studies. Future climate conditions and OA may favor non-calcifying, soft-bodied cnidarians, including zoanthids. Here we show that two zoanthid species, Palythoa sp. and Zoanthus sp., harboring different symbiont types (C1 and A4), had very different responses to increased temperature and increased partial pressure of CO2 (pCO(2)), or dissolved CO2, and low pH. Thermal stress did not affect carbon fixation or fixed carbon translocation in the Zoanthus sp./A4 association, and high pCO(2)/low pH increased carbon fixation. In contrast, both thermal stress and high pCO(2)/low pH greatly inhibited carbon fixation in the Palythoa sp./C1 association. However, the combined treatment of high temperature and high pCO(2) increased carbon fixation relative to the treatment of high temperature alone. Our observations support the growing body of evidence that demonstrates that the response of symbiotic cnidarians to thermal stress and OA must be considered on a host-specific and symbiont-specific basis. In addition, we show that the effects of increased temperature and pCO(2) on photosynthesis may change when these two stressors are combined. Understanding how carbon fixation and translocation varies among different host-symbiont combinations is critical to predicting which Symbiodinium associations may persist in warm, acidified oceans. C1 [Graham, Erin R.; Sanders, Robert W.] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA. [Graham, Erin R.] USDA ARS, Hatfield Marine Sci Ctr, 2030 SE Marine Sci Dr, Newport, OR 97365 USA. RP Graham, ER (reprint author), USDA ARS, Hatfield Marine Sci Ctr, 2030 SE Marine Sci Dr, Newport, OR 97365 USA. EM erin.graham@oregonstate.edu RI Sanders, Robert/C-1116-2011 OI Sanders, Robert/0000-0001-7264-1059 FU National Science Foundation [0838847] FX We wish to thank T. LaJeunesse and J. Reimer for theirhelp with symbiont and host species identification, J. Lunden for his assistance in manipulating carbonate chemistry in aquaria, and T. LaJeunesse, A. Quattrini and Z. McKie-Krisberg for reviewing portions of the manuscript. The initial collection of zoanthids was permitted under the NOAA Office of National Marine Sanctuaries (no. FKNMS-2009-093). This work was partially supported by The National Science Foundation grant 0838847 to R.W.S. NR 111 TC 0 Z9 0 U1 16 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0173-9565 EI 1439-0485 J9 MAR ECOL-EVOL PERSP JI Mar. Ecol.-Evol. Persp. PD APR PY 2016 VL 37 IS 2 BP 442 EP 458 DI 10.1111/maec.12291 PG 17 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DK5RL UT WOS:000374977500017 ER PT J AU Zhang, ZL Zheng, Y Ham, BK Chen, JY Yoshida, A Kochian, LV Fei, ZJ Lucas, WJ AF Zhang, Zhaoliang Zheng, Yi Ham, Byung-Kook Chen, Jieyu Yoshida, Akiko Kochian, Leon V. Fei, Zhangjun Lucas, William J. TI Vascular-mediated signalling involved in early phosphate stress response in plants SO NATURE PLANTS LA English DT Article ID LONG-DISTANCE TRANSPORT; MESSENGER-RNA; GENE-EXPRESSION; BINDING-PROTEIN; WHITE LUPIN; ARABIDOPSIS; PHLOEM; STARVATION; GENOME; PHOSPHORUS AB Depletion of finite global rock phosphate (P-i) reserves will impose major limitations on future agricultural productivity and food security. Hence, modern breeding programmes seek to develop P-i-efficient crops with sustainable yields under reduced P-i fertilizer inputs. In this regard, although the long-term responses of plants to P-i stress are well documented, the early signalling events have yet to be elucidated. Here, we show plant tissue-specific responses to early P-i stress at the transcription level and a predominant role of the plant vascular system in this process. Specifically, imposition of P-i stress induces rapid and major changes in the mRNA population in the phloem translocation stream, and grafting studies have revealed that many hundreds of phloem-mobile mRNAs are delivered to specific sink tissues. We propose that the shoot vascular system acts as the site of root-derived P-i stress perception, and the phloem serves to deliver a cascade of signals to various sinks, presumably to coordinate whole-plant P-i homeostasis. C1 [Zhang, Zhaoliang; Ham, Byung-Kook; Chen, Jieyu; Yoshida, Akiko; Lucas, William J.] Univ Calif Davis, Coll Biol Sci, Dept Plant Biol, Davis, CA 95616 USA. [Zheng, Yi; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [Yoshida, Akiko] Univ Tokyo, Bunkyo Ku, Grad Sch Agr & Life Sci, Dept Agr & Environm Biol, Tokyo 113, Japan. [Kochian, Leon V.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Lucas, WJ (reprint author), Univ Calif Davis, Coll Biol Sci, Dept Plant Biol, Davis, CA 95616 USA. EM wjlucas@ucdavis.edu RI Zheng, Yi/F-6150-2016 OI Zheng, Yi/0000-0002-8042-7770 FU USDA National Institute of Food and Agriculture (NIFA) [201015479]; National Science Foundation [IOS-1339128] FX We thank J. Zeng, W.-c. Hu and S. Zhang for technical support. This work was supported by grants from the USDA National Institute of Food and Agriculture (NIFA; 201015479 to W.J.L. and L.V.K.) and the National Science Foundation (IOS-1339128 to W.J.L.). NR 46 TC 1 Z9 1 U1 23 U2 38 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2055-026X EI 2055-0278 J9 NAT PLANTS JI Nat. Plants PD APR PY 2016 VL 2 IS 4 AR 16033 DI 10.1038/NPLANTS.2016.33 PG 9 WC Plant Sciences SC Plant Sciences GA DL1MZ UT WOS:000375397600006 PM 27249565 ER PT J AU Tiwari, VK Heesacker, A Riera-Lizarazu, O Gunn, H Wang, SC Wang, Y Gu, YQ Paux, E Koo, DH Kumar, A Luo, MC Lazo, G Zemetra, R Akhunov, E Friebe, B Poland, J Gill, BS Kianian, S Leonard, JM AF Tiwari, Vijay K. Heesacker, Adam Riera-Lizarazu, Oscar Gunn, Hilary Wang, Shichen Wang, Yi Gu, Young Q. Paux, Etienne Koo, Dal-Hoe Kumar, Ajay Luo, Ming-Cheng Lazo, Gerard Zemetra, Robert Akhunov, Eduard Friebe, Bernd Poland, Jesse Gill, Bikram S. Kianian, Shahryar Leonard, Jeffrey M. TI A whole-genome, radiation hybrid mapping resource of hexaploid wheat SO PLANT JOURNAL LA English DT Article DE Radiation hybrid mapping; wheat resource; Triticum aestivum; physical mapping; genome assembly; gamma radiation ID TRITICUM-AESTIVUM L.; AEGILOPS-TAUSCHII; PHYSICAL MAP; POLYPLOID WHEAT; CHROMOSOME 3B; DRAFT GENOME; SEQUENCE; GENE; RECOMBINATION; SATURATION AB Generating a contiguous, ordered reference sequence of a complex genome such as hexaploid wheat (2n=6x=42; approximately 17GB) is a challenging task due to its large, highly repetitive, and allopolyploid genome. In wheat, ordering of whole-genome or hierarchical shotgun sequencing contigs is primarily based on recombination and comparative genomics-based approaches. However, comparative genomics approaches are limited to syntenic inference and recombination is suppressed within the pericentromeric regions of wheat chromosomes, thus, precise ordering of physical maps and sequenced contigs across the whole-genome using these approaches is nearly impossible. We developed a whole-genome radiation hybrid (WGRH) resource and tested it by genotyping a set of 115 randomly selected lines on a high-density single nucleotide polymorphism (SNP) array. At the whole-genome level, 26299 SNP markers were mapped on the RH panel and provided an average mapping resolution of approximately 248Kb/cR(1500) with a total map length of 6866 cR(1500). The 7296 unique mapping bins provided a five- to eight-fold higher resolution than genetic maps used in similar studies. Most strikingly, the RH map had uniform bin resolution across the entire chromosome(s), including pericentromeric regions. Our research provides a valuable and low-cost resource for anchoring and ordering sequenced BAC and next generation sequencing (NGS) contigs. The WGRH developed for reference wheat line Chinese Spring (CS-WGRH), will be useful for anchoring and ordering sequenced BAC and NGS based contigs for assembling a high-quality, reference sequence of hexaploid wheat. Additionally, this study provides an excellent model for developing similar resources for other polyploid species. C1 [Tiwari, Vijay K.; Wang, Shichen; Koo, Dal-Hoe; Akhunov, Eduard; Friebe, Bernd; Poland, Jesse; Gill, Bikram S.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Hall, Manhattan, KS 66506 USA. [Heesacker, Adam; Gunn, Hilary; Zemetra, Robert; Leonard, Jeffrey M.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Riera-Lizarazu, Oscar] Dow AgroSci, Pullman, WA USA. [Wang, Yi; Gu, Young Q.; Lazo, Gerard] USDA ARS, Crop Improvement & Genet Res Unit, Albany, NY USA. [Paux, Etienne] INRA, Div & Ecophysiol Cereales, UMR Genet 1095, 5 Chemin Beaulieu, F-63039 Clermont Ferrand, France. [Paux, Etienne] Univ Clermont Ferrand, Div & Ecophysiol Cereales, UMR Genet 1095, F-63177 Aubiere, France. [Kumar, Ajay] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Luo, Ming-Cheng] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Kianian, Shahryar] Univ Minnesota, Cereal Dis Lab, St Paul, MN 55108 USA. RP Tiwari, VK (reprint author), Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Hall, Manhattan, KS 66506 USA.; Leonard, JM (reprint author), Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. EM vijtiwari@ksu.edu; jeff.leonard@oregonstate.edu RI Lazo, Gerard/A-8900-2009; Wang, Shichen/E-6213-2012; OI Lazo, Gerard/0000-0002-9160-2052; Wang, Shichen/0000-0003-1441-2252; Riera-Lizarazu, Oscar/0000-0002-7477-4063; Poland, Jesse/0000-0002-7856-1399 FU National Science Foundation, Plant Genome Research Program (NSF-PGRP) [IOS-082210]; Oregon Wheat Commission; WGRC I/UCRC - NSF grant [IIP-1338897]; NSF [CNS-1006860, EPS-1006860, EPS-0919443] FX This work was supported by funding from the National Science Foundation, Plant Genome Research Program (NSF-PGRP) grant no. IOS-082210. A part of research was also supported by Oregon Wheat Commission. VKT was supported by WGRC I/UCRC partially funded by an NSF grant contract (IIP-1338897). Computational support for this project was provided by the Beocat High-performance Computing Cluster at Kansas State University, which is funded in part by NSF grants CNS-1006860, EPS-1006860, and EPS-0919443. We thank Jon W. Raupp and Nidhi Rawat, WGRC, for critical reading of the manuscript. We also thank Oregon State University undergraduates Lisa Van Ryssegem, Tianxiao Li, and Leman for their help with SNP genotyping. This work represents contribution number from Kansas Agricultural Experiment Station, Kansas State University, Manhattan KS 66506-502, USA. NR 41 TC 3 Z9 3 U1 5 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD APR PY 2016 VL 86 IS 2 BP 195 EP 207 DI 10.1111/tpj.13153 PG 13 WC Plant Sciences SC Plant Sciences GA DK7KK UT WOS:000375103400007 PM 26945524 ER PT J AU Shakoor, N Ziegler, G Dilkes, BP Brenton, Z Boyles, R Connolly, EL Kresovich, S Baxter, I AF Shakoor, Nadia Ziegler, Greg Dilkes, Brian P. Brenton, Zachary Boyles, Richard Connolly, Erin L. Kresovich, Stephen Baxter, Ivan TI Integration of Experiments across Diverse Environments Identifies the Genetic Determinants of Variation in Sorghum bicolor Seed Element Composition SO PLANT PHYSIOLOGY LA English DT Article ID GENOME-WIDE ASSOCIATION; QUANTITATIVE TRAIT LOCUS; ARABIDOPSIS-THALIANA; RICE GRAIN; CHEMICAL-COMPOSITION; NATURAL VARIATION; MODEL APPROACH; IRON UPTAKE; MAIZE; TRANSPORTERS AB Seedling establishment and seed nutritional quality require the sequestration of sufficient element nutrients. The identification of genes and alleles that modify element content in the grains of cereals, including sorghum (Sorghum bicolor), is fundamental to developing breeding and selection methods aimed at increasing bioavailable element content and improving crop growth. We have developed a high-throughput work flow for the simultaneous measurement of multiple elements in sorghum seeds. We measured seed element levels in the genotyped Sorghum Association Panel, representing all major cultivated sorghum races from diverse geographic and climatic regions, and mapped alleles contributing to seed element variation across three environments by genome-wide association. We observed significant phenotypic and genetic correlation between several elements across multiple years and diverse environments. The power of combining high-precision measurements with genome-wide association was demonstrated by implementing rank transformation and a multilocus mixed model to map alleles controlling 20 element traits, identifying 255 loci affecting the sorghum seed ionome. Sequence similarity to genes characterized in previous studies identified likely causative genes for the accumulation of zinc, manganese, nickel, calcium, and cadmium in sorghum seeds. In addition to strong candidates for these five elements, we provide a list of candidate loci for several other elements. Our approach enabled the identification of single-nucleotide polymorphisms in strong linkage disequilibrium with causative polymorphisms that can be evaluated in targeted selection strategies for plant breeding and improvement. C1 [Shakoor, Nadia] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Ziegler, Greg; Baxter, Ivan] USDA ARS, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Dilkes, Brian P.] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA. [Brenton, Zachary; Boyles, Richard; Kresovich, Stephen] Clemson Univ, Dept Biochem & Genet, Clemson, SC 29631 USA. [Connolly, Erin L.] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. RP Baxter, I (reprint author), USDA ARS, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. EM ivan.baxter@ars.usda.gov RI Dilkes, Brian/C-9293-2012; Baxter, Ivan/A-1052-2009; OI Dilkes, Brian/0000-0003-2799-954X; Baxter, Ivan/0000-0001-6680-1722; Shakoor, Nadia/0000-0002-2035-7117; Boyles, Richard/0000-0003-1366-7659 FU National Science Foundation (iHUB Visiting Scientist Program) [DBI 0953433, EAGER 1450341, IOS 1126950, IOS 0919739]; BMGF [OPP 1052924]; Chromatin, Inc. FX This work was supported by the National Science Foundation (iHUB Visiting Scientist Program grant no. DBI 0953433 to David Salt and grant nos. EAGER 1450341 to I.B. and B.P.D., IOS 1126950 to I.B., IOS 0919739 to E.L.C.), the BMGF (grant no. OPP 1052924 to B.P.D.) and Chromatin, Inc.. NR 68 TC 5 Z9 5 U1 6 U2 17 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD APR PY 2016 VL 170 IS 4 BP 1989 EP 1998 DI 10.1104/pp.15.01971 PG 10 WC Plant Sciences SC Plant Sciences GA DL1WR UT WOS:000375424200009 PM 26896393 ER PT J AU Gidda, SK Park, S Pyc, M Yurchenko, O Cai, YQ Wu, P Andrews, DW Chapman, KD Dyer, JM Mullen, RT AF Gidda, Satinder K. Park, Sunjung Pyc, Michal Yurchenko, Olga Cai, Yingqi Wu, Peng Andrews, David W. Chapman, Kent D. Dyer, John M. Mullen, Robert T. TI Lipid Droplet-Associated Proteins (LDAPs) Are Required for the Dynamic Regulation of Neutral Lipid Compartmentation in Plant Cells SO PLANT PHYSIOLOGY LA English DT Article ID RUBBER PARTICLE PROTEINS; ENDOPLASMIC-RETICULUM; OIL BODY; ARABIDOPSIS-THALIANA; CHLAMYDOMONAS-REINHARDTII; MEMBRANE PERMEABILIZATION; SACCHAROMYCES-CEREVISIAE; PHYLOGENETIC ANALYSIS; ACCUMULATION; EXPRESSION AB Eukaryotic cells compartmentalize neutral lipids into organelles called lipid droplets (LDs), and while much is known about the role of LDs in storing triacylglycerols in seeds, their biogenesis and function in nonseed tissues are poorly understood. Recently, we identified a class of plant-specific, lipid droplet-associated proteins (LDAPs) that are abundant components of LDs in nonseed cell types. Here, we characterized the three LDAPs in Arabidopsis (Arabidopsis thaliana) to gain insight to their targeting, assembly, and influence on LD function and dynamics. While all three LDAPs targeted specifically to the LD surface, truncation analysis of LDAP3 revealed that essentially the entire protein was required for LD localization. The association of LDAP3 with LDs was detergent sensitive, but the protein bound with similar affinity to synthetic liposomes of various phospholipid compositions, suggesting that other factors contributed to targeting specificity. Investigation of LD dynamics in leaves revealed that LD abundance was modulated during the diurnal cycle, and characterization of LDAP misexpression mutants indicated that all three LDAPs were important for this process. LD abundance was increased significantly during abiotic stress, and characterization of mutant lines revealed that LDAP1 and LDAP3 were required for the proper induction of LDs during heat and cold temperature stress, respectively. Furthermore, LDAP1 was required for proper neutral lipid compartmentalization and triacylglycerol degradation during postgerminative growth. Taken together, these studies reveal that LDAPs are required for the maintenance and regulation of LDs in plant cells and perform nonredundant functions in various physiological contexts, including stress response and postgerminative growth. C1 [Gidda, Satinder K.; Pyc, Michal; Mullen, Robert T.] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada. [Park, Sunjung; Yurchenko, Olga; Dyer, John M.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Cai, Yingqi; Chapman, Kent D.] Univ N Texas, Ctr Plant Lipid Res, Dept Biol Sci, Denton, TX 76203 USA. [Wu, Peng; Andrews, David W.] Univ Toronto, Sunnybrook Res Inst, Toronto, ON M4N 3M5, Canada. [Wu, Peng; Andrews, David W.] Univ Toronto, Dept Biochem, Toronto, ON M4N 3M5, Canada. RP Mullen, RT (reprint author), Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON N1G 2W1, Canada.; Dyer, JM (reprint author), ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM john.dyer@ars.usda.gov; rtmullen@uoguelph.ca OI Dyer, John/0000-0001-6215-0053; Pyc, Michal/0000-0001-8702-1661; Cai, Yingqi/0000-0002-0357-5809; Chapman, Kent/0000-0003-0489-3072; Andrews, David/0000-0002-9266-7157 FU U.S. Department of Energy, Division of Biological and Environmental Research [DE-FG02-09ER64812/DE-SC0000797]; Natural Sciences and Engineering Research Council of Canada [217291]; University of Guelph (Research Chair); U.S. Department of Agriculture Agricultural Research Service [2020-21000-012-00D]; Canadian Institutes of Health Research [10490]; National Science Foundation [1126205] FX This work was supported by the U.S. Department of Energy, Division of Biological and Environmental Research (grant no. DE-FG02-09ER64812/DE-SC0000797 to K.D.C., J.M.D., and R.T.M.), the Natural Sciences and Engineering Research Council of Canada (grant no. 217291 to R.T.M.) and the University of Guelph (Research Chair to R.T.M.), the U.S. Department of Agriculture Agricultural Research Service (project no. 2020-21000-012-00D to J.M.D.), the Canadian Institutes of Health Research (grant no. 10490 to D.W.A.), and the National Science Foundation (grant no. 1126205 to the University of North Texas). NR 91 TC 5 Z9 5 U1 10 U2 16 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD APR PY 2016 VL 170 IS 4 BP 2052 EP 2071 DI 10.1104/pp.15.01977 PG 20 WC Plant Sciences SC Plant Sciences GA DL1WR UT WOS:000375424200013 PM 26896396 ER PT J AU Manlove, KR Walker, JG Craft, ME Huyvaert, KP Joseph, MB Miller, RS Nol, P Patyk, KA O'Brien, D Walsh, DP Cross, PC AF Manlove, Kezia R. Walker, Josephine G. Craft, Meggan E. Huyvaert, Kathryn P. Joseph, Maxwell B. Miller, Ryan S. Nol, Pauline Patyk, Kelly A. O'Brien, Daniel Walsh, Daniel P. Cross, Paul C. TI "One Health" or Three? Publication Silos Among the One Health Disciplines SO PLOS BIOLOGY LA English DT Article ID DISEASE; INTERDISCIPLINARITY; INFERENCE; SCIENCE; MANAGEMENT; INTERFACE; DYNAMICS; COLLAPSE; GROWTH AB The One Health initiative is a global effort fostering interdisciplinary collaborations to address challenges in human, animal, and environmental health. While One Health has received considerable press, its benefits remain unclear because its effects have not been quantitatively described. We systematically surveyed the published literature and used social network analysis to measure interdisciplinarity in One Health studies constructing dynamic pathogen transmission models. The number of publications fulfilling our search criteria increased by 14.6% per year, which is faster than growth rates for life sciences as a whole and for most biology subdisciplines. Surveyed publications clustered into three communities: one used by ecologists, one used by veterinarians, and a third diverse-authorship community used by population biologists, mathematicians, epidemiologists, and experts in human health. Overlap between these communities increased through time in terms of author number, diversity of co-author affiliations, and diversity of citations. However, communities continue to differ in the systems studied, questions asked, and methods employed. While the infectious disease research community has made significant progress toward integrating its participating disciplines, some segregation-especially along the veterinary/ecological research interface-remains. C1 [Manlove, Kezia R.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Walker, Josephine G.] Univ Bristol, Sch Biol Sci, Bristol, Avon, England. [Craft, Meggan E.] Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA. [Huyvaert, Kathryn P.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Joseph, Maxwell B.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Miller, Ryan S.; Patyk, Kelly A.] Sci Technol & Anal Serv, Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Ft Collins, CO USA. [Nol, Pauline] Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Vet Serv, USDA, Ft Collins, CO USA. [O'Brien, Daniel] Michigan Dept Nat Resources, Wildlife Dis Lab, Lansing, MI USA. [Walsh, Daniel P.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Cross, Paul C.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT USA. RP Manlove, KR (reprint author), Penn State Univ, Dept Biol, University Pk, PA 16802 USA. EM kezia.manlove@gmail.com RI Cross, Paul/K-6987-2012; OI Cross, Paul/0000-0001-8045-5213; Walker, Josephine/0000-0002-9732-5738; Walsh, Daniel/0000-0002-7772-2445; Manlove, Kezia/0000-0002-7200-5236; Miller, Ryan/0000-0003-3892-0251 FU National Science Foundation through NSF [DBI-1300426]; University of Tennessee, Knoxville; Penn State academic computing fellowship; Morris Animal Foundation [D13ZO-081]; University of Bristol; NSF GRFP; Federal Aid in Wildlife Restoration Act under Michigan Pittman-Robertson Project [W-147-R]; Colorado State University; USDA [13-9208-0346-CA]; NSF [DEB-1413925]; Cooperative State Research Service (USDA) [MINV 62-044, 62-051] FX This work was assisted through author participation in the Interface Disease Models Investigative Workshop at the National Institute for Mathematical and Biological Synthesis (NIMBioS), sponsored by the National Science Foundation through NSF Award #DBI-1300426, with additional support from The University of Tennessee, Knoxville. KRM was supported on a Penn State academic computing fellowship and a Morris Animal Foundation grant (D13ZO-081). JGW was supported by a University of Bristol Postgraduate Research Scholarship. MBJ was supported by the NSF GRFP. DO was supported by the Federal Aid in Wildlife Restoration Act under Michigan Pittman-Robertson Project W-147-R. KPH was supported by Colorado State University and USDA Cooperative Agreement #13-9208-0346-CA. MEC was supported by NSF (DEB-1413925) and the Cooperative State Research Service (USDA) under Project Numbers MINV 62-044 and 62-051. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 2 Z9 4 U1 1 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1545-7885 J9 PLOS BIOL JI PLoS. Biol. PD APR PY 2016 VL 14 IS 4 AR e1002448 DI 10.1371/journal.pbio.1002448 PG 14 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA DK7HC UT WOS:000375094800019 PM 27100532 ER PT J AU Lagomasino, D Fatoyinbo, T Lee, S Feliciano, E Trettin, C Simard, M AF Lagomasino, David Fatoyinbo, Temilola Lee, SeungKuk Feliciano, Emanuelle Trettin, Carl Simard, Marc TI A Comparison of Mangrove Canopy Height Using Multiple Independent Measurements from Land, Air, and Space SO REMOTE SENSING LA English DT Article DE canopy height; DSM; biomass; Africa; H100; blue carbon; TDX; VHR; MRV ID POLARIMETRIC SAR INTERFEROMETRY; CARBON-DIOXIDE EMISSIONS; RAIN-FOREST LANDSCAPE; POL-INSAR; TANDEM-X; ZAMBEZI DELTA; TREE HEIGHT; SATELLITE; LIDAR; BIOMASS AB Canopy height is one of the strongest predictors of biomass and carbon in forested ecosystems. Additionally, mangrove ecosystems represent one of the most concentrated carbon reservoirs that are rapidly degrading as a result of deforestation, development, and hydrologic manipulation. Therefore, the accuracy of Canopy Height Models (CHM) over mangrove forest can provide crucial information for monitoring and verification protocols. We compared four CHMs derived from independent remotely sensed imagery and identified potential errors and bias between measurement types. CHMs were derived from three spaceborne datasets; Very-High Resolution (VHR) stereophotogrammetry, TerraSAR-X add-on for Digital Elevation Measurement, and Shuttle Radar Topography Mission (TanDEM-X), and lidar data which was acquired from an airborne platform. Each dataset exhibited different error characteristics that were related to spatial resolution, sensitivities of the sensors, and reference frames. Canopies over 10 m were accurately predicted by all CHMs while the distributions of canopy height were best predicted by the VHR CHM. Depending on the guidelines and strategies needed for monitoring and verification activities, coarse resolution CHMs could be used to track canopy height at regional and global scales with finer resolution imagery used to validate and monitor critical areas undergoing rapid changes. C1 [Lagomasino, David] Univ Space Res Assoc, GESTAR, 7178 Columbia Gateway Dr, Columbia, MD 21046 USA. [Lagomasino, David; Fatoyinbo, Temilola; Lee, SeungKuk; Feliciano, Emanuelle] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Trettin, Carl] USDA, Forest Serv, Cordesville, SC 29434 USA. [Simard, Marc] Jet Prop Lab, Pasadena, CA 91109 USA. RP Lagomasino, D (reprint author), Univ Space Res Assoc, GESTAR, 7178 Columbia Gateway Dr, Columbia, MD 21046 USA.; Lagomasino, D (reprint author), NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. EM david.lagomasino@nasa.gov; lola.fatoyinbo@nasa.gov; seungkuk.lee@nasa.gov; emanuelle.a.feliciano@nasa.gov; ctrettin@fs.fed.us; marc.simard@jpl.nasa.gov RI Fatoyinbo, Temilola/G-6104-2012; Lagomasino, David/P-8413-2015; OI Fatoyinbo, Temilola/0000-0002-1130-6748; Lagomasino, David/0000-0003-4008-5363; Trettin, Carl/0000-0003-0279-7191 FU NASA Carbon Monitoring Systems Program; USAID FX This work was supported in part by the NASA Carbon Monitoring Systems Program. The acquisition of the airborne lidar and MSS data were made possible by funds from USAID support to the US Forest Service International Programs Office under the USAID Mozambique Global Climate Change Sustainable Landscape Program in support of the inventory of carbon stocks in the Zambezi Delta. Commercial high-resolution imagery from DigitalGlobe were obtained from NASA's Commercial Archive Data website (cad4nasa.gsfc.nasa.gov) under the National Geospatial-Intelligence Agency's NextView license agreement. We would like to thank Jaime Nickeson for help in acquiring the commercial imagery from NGA. Part of this work was performed at the Jet Propulsion Laboratory, California Institute of Technology. NR 54 TC 1 Z9 1 U1 11 U2 19 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD APR PY 2016 VL 8 IS 4 AR 327 DI 10.3390/rs8040327 PG 18 WC Remote Sensing SC Remote Sensing GA DK8DD UT WOS:000375156500060 ER PT J AU Simons, G Bastiaanssen, W Ngo, LA Hain, CR Anderson, M Senay, G AF Simons, Gijs Bastiaanssen, Wim Le An Ngo Hain, Christopher R. Anderson, Martha Senay, Gabriel TI Integrating Global Satellite-Derived Data Products as a Pre-Analysis for Hydrological Modelling Studies: A Case Study for the Red River Basin SO REMOTE SENSING LA English DT Article DE global satellite-derived data; intercomparison; evapotranspiration; Red River Basin; hydrological modeling; water accounting ID REMOTELY-SENSED EVAPOTRANSPIRATION; WATER-BALANCE; SURFACE-WATER; TIME-SERIES; NILE BASIN; RAINFALL; MODIS; CONSUMPTION; EVAPORATION; RESOLUTION AB With changes in weather patterns and intensifying anthropogenic water use, there is an increasing need for spatio-temporal information on water fluxes and stocks in river basins. The assortment of satellite-derived open-access information sources on rainfall (P) and land use/land cover (LULC) is currently being expanded with the application of actual evapotranspiration (ETact) algorithms on the global scale. We demonstrate how global remotely sensed P and ETact datasets can be merged to examine hydrological processes such as storage changes and streamflow prior to applying a numerical simulation model. The study area is the Red River Basin in China in Vietnam, a generally challenging basin for remotely sensed information due to frequent cloud cover. Over this region, several satellite-based P and ETact products are compared, and performance is evaluated using rain gauge records and longer-term averaged streamflow. A method is presented for fusing multiple satellite-derived ETact estimates to generate an ensemble product that may be less susceptible, on a global basis, to errors in individual modeling approaches. Subsequently, monthly satellite-derived rainfall and ETact are combined to assess the water balance for individual subcatchments and types of land use, defined using a global land use classification improved based on auxiliary satellite data. It was found that a combination of TRMM rainfall and the ensemble ETact product is consistent with streamflow records in both space and time. It is concluded that monthly storage changes, multi-annual streamflow and water yield per LULC type in the Red River Basin can be successfully assessed based on currently available global satellite-derived products. C1 [Simons, Gijs; Bastiaanssen, Wim] Delft Univ Technol, Dept Water Management, Fac Civil Engn & Geosci, Stevinweg 1, NL-2628 CN Delft, Netherlands. [Simons, Gijs] FutureWater, Costerweg 1V, NL-6702 AA Wageningen, Netherlands. [Bastiaanssen, Wim] UNESCO IHE, Westvest 7, NL-2611 AX Delft, Netherlands. [Le An Ngo] Thuy Loi Univ, Fac Hydrol & Water Resources, 175 Tay Son, Hanoi, Vietnam. [Hain, Christopher R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Anderson, Martha] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Senay, Gabriel] Colorado State Univ, North Cent Climate Sci Ctr, USGS EROS Ctr, Ft Collins, CO 80523 USA. RP Simons, G (reprint author), Delft Univ Technol, Dept Water Management, Fac Civil Engn & Geosci, Stevinweg 1, NL-2628 CN Delft, Netherlands.; Simons, G (reprint author), FutureWater, Costerweg 1V, NL-6702 AA Wageningen, Netherlands. EM g.w.h.simons@tudelft.nl; wim.bastiaanssen@gmail.com; annl@wru.vn; Chris.Hain@noaa.gov; martha.anderson@ars.usda.gov; senay@usgs.gov RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU CGIAR Water Land and Ecosystems (WLE) [MK27]; Delft University of Technology; Government of The Netherlands FX Part of this research was funded by the CGIAR Water Land and Ecosystems (WLE) MK27 program. Funds for publishing in open access were provided by the Open Access Fund of Delft University of Technology. The authors are grateful to the following persons and institutes for making available the global ETact products: Albert van Dijk (University of Australia), Juan Pablo Guerschman (CSIRO) and Jorge Pena Arancibia (CSIRO) for CMRSET, and Xuelong Chen (ITC) for SEBS. Hydrometeorological data were acquired in the framework of the project Demonstration of Remote Sensing Information for Integrated Reservoir Management in the Red River Basin in Northern Vietnam, funded by the Government of The Netherlands. The authors are thankful to Dung Duc Le and Duc Anh Nguyen of VINWATER for their assistance in acquiring, processing and interpreting the hydrometeorological data, and to Lan Thanh Ha of IWRP for his valuable inputs. NR 102 TC 2 Z9 2 U1 2 U2 5 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD APR PY 2016 VL 8 IS 4 AR UNSP 279 DI 10.3390/rs8040279 PG 28 WC Remote Sensing SC Remote Sensing GA DK8DD UT WOS:000375156500012 ER PT J AU Huang, M Kim, MS Chao, KL Qin, JW Mo, CY Esquerre, C Delwiche, S Zhu, QB AF Huang, Min Kim, Moon S. Chao, Kuanglin Qin, Jianwei Mo, Changyeun Esquerre, Carlos Delwiche, Stephen Zhu, Qibing TI Penetration Depth Measurement of Near-Infrared Hyperspectral Imaging Light for Milk Powder SO SENSORS LA English DT Article DE penetration depth; hyperspectral imaging; milk powder; PLS-DA ID MELAMINE DETECTION; DAIRY-PRODUCTS; INFANT FORMULA; SPECTROSCOPY; REFLECTANCE; SPECTROMETRY; QUALITY; SYSTEM; FRUIT AB The increasingly common application of the near-infrared (NIR) hyperspectral imaging technique to the analysis of food powders has led to the need for optical characterization of samples. This study was aimed at exploring the feasibility of quantifying penetration depth of NIR hyperspectral imaging light for milk powder. Hyperspectral NIR reflectance images were collected for eight different milk powder products that included five brands of non-fat milk powder and three brands of whole milk powder. For each milk powder, five different powder depths ranging from 1 mm-5 mm were prepared on the top of a base layer of melamine, to test spectral-based detection of the melamine through the milk. A relationship was established between the NIR reflectance spectra (937.5-1653.7 nm) and the penetration depth was investigated by means of the partial least squares-discriminant analysis (PLS-DA) technique to classify pixels as being milk-only or a mixture of milk and melamine. With increasing milk depth, classification model accuracy was gradually decreased. The results from the 1-mm, 2-mm and 3-mm models showed that the average classification accuracy of the validation set for milk-melamine samples was reduced from 99.86% down to 94.93% as the milk depth increased from 1 mm-3 mm. As the milk depth increased to 4 mm and 5 mm, model performance deteriorated further to accuracies as low as 81.83% and 58.26%, respectively. The results suggest that a 2-mm sample depth is recommended for the screening/evaluation of milk powders using an online NIR hyperspectral imaging system similar to that used in this study. C1 [Huang, Min; Zhu, Qibing] Jiangnan Univ, Minist Educ, Key Lab Adv Proc Control Light Ind, Wuxi 214122, Peoples R China. [Huang, Min; Kim, Moon S.; Chao, Kuanglin; Qin, Jianwei] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Mo, Changyeun] Natl Inst Agr Sci, Rural Dev Adm, 310 Nongsaengmyeong Ro, Jueonju Si 54875, Jeollabuk Do, South Korea. [Esquerre, Carlos] Univ Coll Dublin, Sch Biosyst & Food Engn, Dublin 4, Ireland. [Esquerre, Carlos; Delwiche, Stephen] ARS, Food Qual, USDA, Beltsville, MD 20705 USA. RP Kim, MS (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. EM huangmzqb@163.com; moon.kim@ars.usda.gov; kevin.chao@ars.usda.gov; jianwei.qin@ars.usda.gov; cymoh100@korea.kr; carlos.esquerre@ucd.ie; stephen.delwiche@ars.usda.gov; zhuqib@163.com FU Agenda Programs [PJ009399, PJ012216]; Rural Development Administration, Korea; National Natural Science Foundation of China [61271384, 61275155]; Qing Lan Projects of China; [B12018] FX This work was partially supported by grants from the Agenda Programs (PJ009399) and (PJ012216), Rural Development Administration, Korea, the National Natural Science Foundation of China (Grant # 61271384 and 61275155), and the 111 (B12018) and Qing Lan Projects of China. The authors would like to thank Diane Chan of Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, USDA, for reviewing the manuscript. NR 25 TC 0 Z9 0 U1 9 U2 11 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD APR PY 2016 VL 16 IS 4 AR 441 DI 10.3390/s16040441 PG 11 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA DK8CB UT WOS:000375153700021 PM 27023555 ER PT J AU de Bruyn, LL Andrews, S AF de Bruyn, Lisa Lobry Andrews, Susan TI Are Australian and United States Farmers Using Soil Information for Soil Health Management? SO SUSTAINABILITY LA English DT Review DE soil health; soil monitoring; soil information; soil testing; farmers ID QUALITY INDICATORS; ADOPTION; PERCEPTIONS; DEGRADATION AB Soil health is an essential requirement of a sustainable, functioning agroecosystem. Tracking soil health to determine sustainability at the local level largely falls to farmers, even though they often lack access to critical information. We examine farmers' participation in gathering soil information at the farm and paddock scale over the last two decades in Australia and the United States, by reviewing national-level reporting of farmer use of soil testing and farm planning as well as qualitative research on farmer perspectives. The level of participation in soil testing and farm planning has remained stable in the last two decades, with only 25% and 30% of landholders, respectively, participating nationally, in either country. The review revealed national-level reporting has a number of limitations in understanding farmers' use of soil information and, in particular, fails to indicate the frequency and intensity of soil testing as well as farmer motivation to test soil or what they did with the soil information. The main use of soil testing is often stated as "determining fertilizer requirements", yet data show soil testing is used less commonly than is customary practice. In Australia and in the United States, customary practice is three and half times more likely for decisions on fertilizer application levels. The rhetoric is heavy on the use of soil testing as a decision tool, and that it guides best practices. However, given that only a quarter of farmers are soil testing, and doing so infrequently and in low densities, the level of information on soil health is poor. While farmers report consistent monitoring of soil conditions, few have consistent records of such. In contrast to the information on the poor state of soil health, there is strong farmer interest in procuring soil health benefits through changes in farm practices such as conservation tillage or cover crops, even if they are unable to demonstrate these soil health benefits through soil testing. Many farmers report the use of observation in lieu of laboratory testing. Finally, we point to the need for soil information to include observational indicators to best allow a blend of traditional extension strategies with digital technology to create communities of interest in soil management. This would transcend the boundaries between those with expertise and those with experience in soil health management. C1 [de Bruyn, Lisa Lobry] Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia. [Andrews, Susan] Nat Resources Conservat Serv, USDA, Denton, NE 68339 USA. RP de Bruyn, LL (reprint author), Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia. EM llobryde@une.edu.au; susan.andrews@lin.usda.gov OI Lobry de Bruyn, Lisa/0000-0003-0173-2863 NR 126 TC 1 Z9 1 U1 7 U2 21 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD APR PY 2016 VL 8 IS 4 AR 304 DI 10.3390/su8040304 PG 33 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DK8CW UT WOS:000375155800014 ER PT J AU Kim, G Miller, P Nowak, D AF Kim, Gunwoo Miller, Patrick Nowak, David TI The Value of Green Infrastructure on Vacant and Residential Land in Roanoke, Virginia SO SUSTAINABILITY LA English DT Article DE urban forestry; ecosystem services; green infrastructure ID URBAN; DEPOSITION; SERVICES; CANOPY; MODEL AB Using the City of Roanoke, Virginia as a study site, this paper quantifies the forest structure, ecosystem services and values of vacant and residential land. Single family residential land had more trees (1,683,000) than vacant land (210,000) due largely to the differences in land area (32.44 km(2) of vacant land vs. 57.94 km(2) residential). While the percentage of tree coverage was almost identical across land uses (30.6% in vacant to 32.3% in residential), the number of trees per ha is greater on residential land (290.3) than on vacant land (63.4). The average healthy leaf surface area on individual trees growing on vacant land was greater than that of individual trees on residential land. The fact that trees in vacant land were found to provide more ecosystem services per tree than residential trees was attributed to this leaf area difference. Trees on vacant land are growing in more natural conditions and there are more large trees per ha. Assessing the forest structure and ecosystem services of Roanoke's vacant and residential land provides a picture of the current extent and condition of the vacant and residential land. Understanding these characteristics provides the information needed for improved management and utilization of urban vacant land and estimating green infrastructure value. C1 [Kim, Gunwoo] Arizona State Univ, Landscape Architecture Program, POB 871605, Tempe, AZ 85287 USA. [Miller, Patrick] Virginia Polytech Inst & State Univ, Landscape Architecture Program, 120 F Burruss Hall 0190, Blacksburg, VA 24061 USA. [Nowak, David] US Forest Serv, USDA, No Res Stn, SUNY ESF,Moon Lib 5, Syracuse, NY 13215 USA. RP Kim, G (reprint author), Arizona State Univ, Landscape Architecture Program, POB 871605, Tempe, AZ 85287 USA. EM Gunwoo.Kim.1@asu.edu; pmiller@vt.edu; dnowak@fs.fed.us FU Virginia Tech's Open Access Subvention Fund (OASF); APS Endowment for Sustainable Design Research Fund (Arizona State University, Herberger Institute for Design and the Arts) FX The open access publishing fees for this article have been covered by the Virginia Tech's Open Access Subvention Fund (OASF), supported by the University Libraries. This research was supported by funds through the APS Endowment for Sustainable Design Research Fund (Arizona State University, Herberger Institute for Design and the Arts). NR 34 TC 3 Z9 3 U1 8 U2 14 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD APR PY 2016 VL 8 IS 4 AR 296 DI 10.3390/su8040296 PG 15 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DK8CW UT WOS:000375155800006 ER PT J AU Yen, H Daggupati, P White, MJ Srinivasan, R Gossel, A Wells, D Arnold, JG AF Yen, Haw Daggupati, Prasad White, Michael J. Srinivasan, Raghavan Gossel, Arndt Wells, David Arnold, Jeffrey G. TI Application of Large-Scale, Multi-Resolution Watershed Modeling Framework Using the Hydrologic and Water Quality System (HAWQS) SO WATER LA English DT Article DE decision support system; watershed modeling; web-based application; model calibration; SWAT ID ASSESSMENT-TOOL; CALIBRATION; VALIDATION; SIMULATIONS; IMPACT; SWAT AB In recent years, large-scale watershed modeling has been implemented broadly in the field of water resources planning and management. Complex hydrological, sediment, and nutrient processes can be simulated by sophisticated watershed simulation models for important issues such as water resources allocation, sediment transport, and pollution control. Among commonly adopted models, the Soil and Water Assessment Tool (SWAT) has been demonstrated to provide superior performance with a large amount of referencing databases. However, it is cumbersome to perform tedious initialization steps such as preparing inputs and developing a model with each changing targeted study area. In this study, the Hydrologic and Water Quality System (HAWQS) is introduced to serve as a national-scale Decision Support System (DSS) to conduct challenging watershed modeling tasks. HAWQS is a web-based DSS developed and maintained by Texas A & M University, and supported by the U.S. Environmental Protection Agency. Three different spatial resolutions of Hydrologic Unit Code (HUC8, HUC10, and HUC12) and three temporal scales (time steps in daily/monthly/annual) are available as alternatives for general users. In addition, users can specify preferred values of model parameters instead of using the pre-defined sets. With the aid of HAWQS, users can generate a preliminarily calibrated SWAT project within a few minutes by only providing the ending HUC number of the targeted watershed and the simulation period. In the case study, HAWQS was implemented on the Illinois River Basin, USA, with graphical demonstrations and associated analytical results. Scientists and/or decision-makers can take advantage of the HAWQS framework while conducting relevant topics or policies in the future. C1 [Yen, Haw] Texas A&M Univ, Blackland Res & Extens Ctr, Texas A&M Agrilife Res, 720 East Blackland Rd, Temple, TX 76502 USA. [Daggupati, Prasad; Srinivasan, Raghavan] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA. [White, Michael J.; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. [Gossel, Arndt] US EPA, Oak Ridge Inst Sci & Educ, Off Water, Washington, DC 20460 USA. [Wells, David] US EPA, Off Water, Washington, DC 20460 USA. RP Yen, H (reprint author), Texas A&M Univ, Blackland Res & Extens Ctr, Texas A&M Agrilife Res, 720 East Blackland Rd, Temple, TX 76502 USA. EM haw.yen@gmail.com; pdaggupati@tamu.edu; mike.white@ars.usda.gov; r-srinivasan@tamu.edu; gossel.arndt@epa.gov; dwells@his.com; jeff.arnold@ars.usda.gov RI Srinivasan, R/D-3937-2009; Daggupati, Prasad/D-8886-2017 OI Daggupati, Prasad/0000-0002-7044-3435 FU U.S. Environmental Protection Agency; United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components FX This project was funded by grants from (i) U.S. Environmental Protection Agency; and (ii) United States Department of Agriculture-Natural Resources Conservation Service (USDA-NRCS) Conservation Effects Assessment Project (CEAP)-Wildlife and Cropland components. The views expressed in this presentation are those of the authors and do not necessarily reflect the views or policies of the United States Environmental Protection Agency and the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 38 TC 3 Z9 3 U1 5 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD APR PY 2016 VL 8 IS 4 AR 164 DI 10.3390/w8040164 PG 23 WC Water Resources SC Water Resources GA DK8DK UT WOS:000375157200055 ER PT J AU Molin, WT Nandula, VK Wright, AA Bond, JA AF Molin, William T. Nandula, Vijay K. Wright, Alice A. Bond, Jason A. TI Transfer and Expression of ALS Inhibitor Resistance from Palmer Amaranth (Amaranthus palmeri) to an A. spinosus x A. palmeri Hybrid SO WEED SCIENCE LA English DT Article DE Acetolactate synthase; ALS enzyme; cross resistance; hybrid; mutation ID MULTIPLE RESISTANCE; FIELD CONDITIONS; GLYPHOSATE; PIGWEED; HYBRIDIZATION; TUBERCULATUS; PYRITHIOBAC; HERBICIDES AB Transfer of herbicide resistance among closely related weed species is a topic of growing concern. A spiny amaranth 6 Palmer amaranth hybrid was confirmed resistant to several acetolactate synthase (ALS) inhibitors including imazethapyr, nicosulfuron, pyrithiobac, and trifloxysulfuron. Enzyme assays indicated that the ALS enzyme was insensitive to pyrithiobac and sequencing revealed the presence of a known resistance conferring point mutation, Trp574Leu. Alignment of the ALS gene for Palmer amaranth, spiny amaranth, and putative hybrids revealed the presence of Palmer amaranth ALS sequence in the hybrids rather than spiny amaranth ALS sequences. In addition, sequence upstream of the ALS in the hybrids matched Palmer amaranth and not spiny amaranth. The potential for transfer of ALS inhibitor resistance by hybridization has been demonstrated in the greenhouse and in field experiments. This is the first report of gene transfer for ALS inhibitor resistance documented to occur in the field without artificial/human intervention. These results highlight the need to control related species in both field and surrounding noncrop areas to avoid interspecific transfer of resistance genes. C1 [Molin, William T.; Nandula, Vijay K.; Wright, Alice A.] ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. [Bond, Jason A.] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA. RP Molin, WT (reprint author), ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. EM william.molin@ars.usda.gov NR 21 TC 1 Z9 1 U1 4 U2 7 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2016 VL 64 IS 2 BP 240 EP 247 DI 10.1614/WS-D-15-00172.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DK8DV UT WOS:000375158300005 ER PT J AU Hill, EC Renner, KA Sprague, CL Davis, AS AF Hill, Erin C. Renner, Karen A. Sprague, Christy L. Davis, Adam S. TI Cover Crop Impact on Weed Dynamics in an Organic Dry Bean System SO WEED SCIENCE LA English DT Article DE Cover-crop nitrogen dynamics; soil amendments; structural equation modeling (SEM); weed density; weed seed persistence ID FOXTAIL SETARIA-FABERI; VELVETLEAF ABUTILON-THEOPHRASTI; GREEN MANURE; NITROGEN-FERTILIZER; SEED MORTALITY; BENZOXAZINOID COMPOUNDS; RESIDUE MANAGEMENT; SOIL-NITROGEN; SWEET CORN; GROWTH AB Weed suppression is one possible benefit of including cover crops in crop rotations. The late spring planting date of dry beans allows for more growth of cover crops in the spring. We assessed the influence of cover crops on weed dynamics in organic dry beans and weed seed persistence. Medium red clover, oilseed radish, and cereal rye were planted the year before dry beans; a no-cover-crop control was also included. After cover-crop incorporation, common lambsquarters, giant foxtail, and velvetleaf seeds were buried in the red clover, cereal rye, and no-cover control treatments and then retrieved 0, 1, 2, 4, 6, and 12 mo after cover-crop incorporation. Dry beans were planted in June and weed emergence and biomass measured. Eleven or more site-years of data were collected for each cover-crop treatment between 2011 and 2013, allowing for structural equation modeling (SEM), in addition to traditional analyses. Cereal rye residue increased giant foxtail and velvetleaf seed persistence by up to 12%; red clover decreased common lambsquarters seed persistence by 22% in 1 of 2 yr relative to the no-cover-crop control. Oilseed radish and incorporated cereal rye rarely reduced weed densities. When red clover biomass exceeded 5 Mg ha(-1), soil inorganic N was often higher (5 of 6 site-years), as were weed density and biomass (5 and 4 of 12 main site sample times, respectively). Using SEM, we identified one causal relationship between cover-crop N content and weed biomass at the first flower stage (R1), as mediated through soil N at the time of dry bean planting and at the stage with two fully expanded trifoliates. Increasing cover-crop C : N ratios directly reduced weed biomass at R1, not mediated through changes in soil N. Cover crops that make a significant contribution to soil N may also stimulate weed emergence and growth. C1 [Hill, Erin C.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. ARS, USDA, Urbana, IL 61801 USA. RP Hill, EC (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. EM hiller12@msu.edu FU Organic Research and Extension Initiative of the National Institute of Food and Agriculture, U.S. Department of Agriculture [2010-51300-21224]; Northeast Regional Association Project [NE-1047] FX This material is based upon work that is supported by the Organic Research and Extension Initiative of the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2010-51300-21224. We acknowledge the Northeast Regional Association Project NE-1047: Ecological bases for weed management in sustainable cropping systems participants for collaboration and support, and acknowledge several Michigan organic dry bean producers who conducted research on their farms. NR 87 TC 0 Z9 0 U1 12 U2 18 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2016 VL 64 IS 2 BP 261 EP 275 DI 10.1614/WS-D-15-00114.1 PG 15 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DK8DV UT WOS:000375158300007 ER PT J AU Berger, S Madeira, PT Ferrell, J Gettys, L Morichetti, S Cantero, JJ Nunez, C AF Berger, Sarah Madeira, Paul T. Ferrell, Jason Gettys, Lyn Morichetti, Sergio Cantero, Juan J. Nunez, Cesar TI Palmer Amaranth (Amaranthus palmeri) Identification and Documentation of ALS-Resistance in Argentina SO WEED SCIENCE LA English DT Article DE Acetolactate synthase (ALS)-inhibiting herbicides; dose response; molecular taxonomy ID INHIBITING HERBICIDES; CROSS-RESISTANCE; GRAIN AMARANTHS; GROWTH; PCR; HYBRIDIZATION; RETROFLEXUS; QUITENSIS; GENETICS; MARKERS AB Palmer amaranth has greatly disrupted agricultural practices in the United States with its rapid growth and rapid evolution of herbicide resistance. This weed species is now suspected in Argentina. To document whether the suspected plant populations are indeed Palmer amaranth, molecular comparisons to known standards were conducted. Additionally, these same plant populations were screened for possible herbicide resistance to several acetolactate synthase (ALS)-inhibiting herbicides. Sequencing data confirmed that suspected populations (A2, A3, A4) were indeed Palmer amaranth. Another population (A1) was tested to determine whether hybridization had occurred between Palmer amaranth and mucronate amaranth the native amaranth species of the region. Tests confirmed that no hybridization had occurred and that A1 was simply a unique phenotype of mucronate amaranth. Each population was screened for resistance to imazapic, nicosulfuron, and diclosulam. All Palmer amaranth populations from Argentina were shown to be resistant to at least one ALS-inhibiting herbicide. The populations were then subjected to further testing to identify the mutation responsible for the observed ALS resistance. All mucronate amaranth populations exhibited a mutation previously documented to confer ALS resistance (S653N). No known resistance-conferring mutations were found in Palmer amaranth. C1 [Berger, Sarah; Ferrell, Jason] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Madeira, Paul T.] USDA, Invas Plant Res Lab, Davie, FL 33314 USA. [Gettys, Lyn] Univ Florida, Dept Agron, Ft Lauderdale REC, Davie, FL 33314 USA. [Morichetti, Sergio] Aceitera Gen Deheza SA, Gen Deheza, Cordoba, Argentina. [Cantero, Juan J.; Nunez, Cesar] UNRC, Fac Agron & Vet, Dept Biol Agr, Cordoba, Argentina. RP Ferrell, J (reprint author), Univ Florida, Dept Agron, Gainesville, FL 32611 USA. EM jferrell@ufl.edu NR 44 TC 0 Z9 0 U1 5 U2 8 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2016 VL 64 IS 2 BP 312 EP 320 DI 10.1614/WS-D-15-00125.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DK8DV UT WOS:000375158300012 ER PT J AU Regnier, EE Harrison, SK Loux, MM Holloman, C Venkatesh, R Diekmann, F Taylor, R Ford, RA Stoltenberg, DE Hartzler, RG Davis, AS Schutte, BJ Cardina, J Mahoney, KJ Johnson, WG AF Regnier, Emilie E. Harrison, S. Kent Loux, Mark M. Holloman, Christopher Venkatesh, Ramarao Diekmann, Florian Taylor, Robin Ford, Robert A. Stoltenberg, David E. Hartzler, Robert G. Davis, Adam S. Schutte, Brian J. Cardina, John Mahoney, Kris J. Johnson, William G. TI Certified Crop Advisors' Perceptions of Giant Ragweed (Ambrosia trifida) Distribution, Herbicide Resistance, and Management in the Corn Belt SO WEED SCIENCE LA English DT Article DE Allergy; asthma; crop rotation; nightcrawler; postemergence herbicide; tillage practice; weed adaptation; weed emergence ID ARABLE WEED POPULATIONS; SOYBEAN GLYCINE-MAX; SEEDLING EMERGENCE; HERBARIUM RECORDS; ARTEMISIIFOLIA L.; YIELD LOSS; GLYPHOSATE; COMPETITION; ARKANSAS; SPREAD AB Giant ragweed has been increasing as a major weed of row crops in the last 30 yr, but quantitative data regarding its pattern and mechanisms of spread in crop fields are lacking. To address this gap, we conducted a Web-based survey of certified crop advisors in the U.S. Corn Belt and Ontario, Canada. Participants were asked questions regarding giant ragweed and crop production practices for the county of their choice. Responses were mapped and correlation analyses were conducted among the responses to determine factors associated with giant ragweed populations. Respondents rated giant ragweed as the most or one of the most difficult weeds to manage in 45% of 421 U.S. counties responding, and 57% of responding counties reported giant ragweed populations with herbicide resistance to acetolactate synthase inhibitors, glyphosate, or both herbicides. Results suggest that giant ragweed is increasing in crop fields outward from the east-central U.S. Corn Belt in most directions. Crop production practices associated with giant ragweed populations included minimum tillage, continuous soybean, and multiple-application herbicide programs; ecological factors included giant ragweed presence in noncrop edge habitats, early and prolonged emergence, and presence of the seed-burying common earthworm in crop fields. Managing giant ragweed in noncrop areas could reduce giant ragweed migration from noncrop habitats into crop fields and slow its spread. Where giant ragweed is already established in crop fields, including a more diverse combination of crop species, tillage practices, and herbicide sites of action will be critical to reduce populations, disrupt emergence patterns, and select against herbicide-resistant giant ragweed genotypes. Incorporation of a cereal grain into the crop rotation may help suppress early giant ragweed emergence and provide chemical or mechanical control options for late-emerging giant ragweed. C1 [Regnier, Emilie E.; Harrison, S. Kent; Loux, Mark M.; Venkatesh, Ramarao; Ford, Robert A.] Ohio State Univ, Dept Hort & Crop Sci, 2021 Coffey Rd, Columbus, OH 43210 USA. [Holloman, Christopher] Ohio State Univ, Stat Consulting Serv, Dept Stat, 1958 Neil Ave, Columbus, OH 43210 USA. [Diekmann, Florian] Ohio State Univ Lib, 2120 Fyffe Rd, Columbus, OH 43210 USA. [Taylor, Robin] Texas A&M AgriLife Res & Extens Ctr, 720 E Blackland Rd, Temple, TX 76502 USA. [Stoltenberg, David E.] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Hartzler, Robert G.] Iowa State Univ, Dept Agron, 2104 Agron Hall, Ames, IA 50011 USA. [Davis, Adam S.] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Schutte, Brian J.] New Mexico State Univ, Dept Entomol Plant Pathol & Weed Sci, 945 Coll Ave, Las Cruces, NM 88011 USA. [Cardina, John] Ohio State Univ, Ohio Agr & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Mahoney, Kris J.] Univ Guelph, Dept Plant Agr, Ridgetown Campus, Ridgetown, ON N0P 2C0, Canada. [Johnson, William G.] Purdue Univ, Dept Bot & Plant Pathol, 915 West State St, W Lafayette, IN 47907 USA. RP Regnier, EE (reprint author), Ohio State Univ, Dept Hort & Crop Sci, 2021 Coffey Rd, Columbus, OH 43210 USA. EM regnier.1@osu.edu FU National Research Initiative of the U.S. Department of Agriculture Cooperative State Research, Education and Extension Service [11073659, 10356701]; [2011-078] FX This project was supported by the National Research Initiative of the U.S. Department of Agriculture Cooperative State Research, Education and Extension Service, GRANT11073659 and GRANT10356701. We are grateful for the efforts of Dr. Christopher Boerboom to secure the initial funding and the professional facilitation of the research team by Dr. Joseph Heimlich. Salaries and research support were provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, grant number 2011-078. Journal article HCS15-18. NR 65 TC 1 Z9 1 U1 5 U2 17 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2016 VL 64 IS 2 BP 361 EP 377 DI 10.1614/WS-D-15-00116.1 PG 17 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DK8DV UT WOS:000375158300017 ER PT J AU Van Beusekom, AE Gonzalez, G Rivera, MM AF Van Beusekom, Ashley E. Gonzalez, Grizelle Rivera, Maria M. TI Short-term precipitation and temperature trends along an elevation gradient in Northeastern Puerto Rico (vol 19, pg 1, 2015) SO EARTH INTERACTIONS LA English DT Correction C1 [Van Beusekom, Ashley E.; Gonzalez, Grizelle; Rivera, Maria M.] US Forest Serv, USDA, Int Inst Trop Forestry, Rio Piedras, PR USA. RP Van Beusekom, AE (reprint author), US Forest Serv, SDA, Int Inst Trop Forestry, Jardin Bot Sur, 1201 Calle Ceiba, Rio Piedras, PR 00926 USA. EM ashley.vanbeusekom@gmail.com OI Van Beusekom, Ashley/0000-0002-6996-978X NR 1 TC 0 Z9 0 U1 0 U2 1 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 1087-3562 J9 EARTH INTERACT JI Earth Interact. PD APR PY 2016 VL 20 DI 10.1175/EI-D-15-0044.1 PG 2 WC Geosciences, Multidisciplinary SC Geology GA DK0ZR UT WOS:000374642200001 ER PT J AU Chen, YW Sagar, V Len, HS Peterson, K Fan, JG Mishra, S McMurtry, J Wilmarth, PA David, LL Wistow, G AF Chen, Yingwei Sagar, Vatsala Len, Hoay-Shuen Peterson, Katherine Fan, Jianguo Mishra, Sanghamitra McMurtry, John Wilmarth, Phillip A. David, Larry L. Wistow, Graeme TI gamma-Crystallins of the chicken lens: remnants of an ancient vertebrate gene family in birds SO FEBS JOURNAL LA English DT Article DE crystallin; evolution; eye; promoter; protein folding; proteomics ID SEQUENCE TAG ANALYSIS; S-CRYSTALLIN; EYE LENS; NEIBANK PROJECT; SEARCH TOOL; EXPRESSION; PROTEIN; EVOLUTION; RECRUITMENT; ENZYME AB gamma-Crystallins, abundant proteins of vertebrate lenses, were thought to be absent from birds. However, bird genomes contain well-conserved genes for S- and N-crystallins. Although expressed sequence tag analysis of chicken eye found no transcripts for these genes, RT-PCR detected spliced transcripts for both genes in chicken lens, with lower levels in cornea and retina/retinal pigment epithelium. The level of mRNA for S in chicken lens was relatively very low even though the chicken crygs gene promoter had lens-preferred activity similar to that of mouse. Chicken S was detected by a peptide antibody in lens, but not in other ocular tissues. Low levels of S and N proteins were detected in chicken lens by shotgun mass spectroscopy. Water-soluble and water-insoluble lens fractions were analyzed and 1934 proteins (< 1% false discovery rate) were detected, increasing the known chicken lens proteome 30-fold. Although chicken S is well conserved in protein sequence, it has one notable difference in leucine 16, replacing a surface glutamine conserved in other -crystallins, possibly affecting solubility. However, L16 and engineered Q16 versions were both highly soluble and had indistinguishable circular dichroism, tryptophan fluorescence and heat stability (melting temperature T-m similar to 65 degrees C) profiles. L16 has been present in birds for over 100 million years and may have been adopted for a specific protein interaction in the bird lens. However, evolution has clearly reduced or eliminated expression of ancestral -crystallins in bird lenses. The conservation of genes for S- and N-crystallins suggests they may have been preserved for reasons unrelated to the bulk properties of the lens. C1 [Chen, Yingwei; Sagar, Vatsala; Len, Hoay-Shuen; Peterson, Katherine; Fan, Jianguo; Mishra, Sanghamitra; Wistow, Graeme] NEI, Sect Mol Struct & Funct Genom, NIH, Bg 6,Rm 106, Bethesda, MD 20892 USA. [McMurtry, John] ARS, USDA, Southern Plains Area, East Coll Stn, Dallas, TX USA. [Wilmarth, Phillip A.; David, Larry L.] Oregon Hlth & Sci Univ, Dept Biochem & Mol Biol, Sch Med, Portland, OR 97201 USA. RP Wistow, G (reprint author), NEI, Sect Mol Struct & Funct Genom, NIH, Bg 6,Rm 106, Bethesda, MD 20892 USA. EM graeme@helix.nih.gov FU NEI Intramural Program; NIH [EY010572, EY007755] FX This work was performed with the support of the NEI Intramural Program and NIH grants EY010572 and EY007755. Some experiments were performed at the NHLBI Biophysics Core. NR 58 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1742-464X EI 1742-4658 J9 FEBS J JI FEBS J. PD APR PY 2016 VL 283 IS 8 BP 1516 EP 1530 DI 10.1111/febs.13689 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DK1NV UT WOS:000374680500012 PM 26913478 ER PT J AU Luedtke, BE Bosilevac, JM Harhay, DM Arthur, TM AF Luedtke, Brandon E. Bosilevac, Joseph M. Harhay, Dayna M. Arthur, Terrance M. TI Effect of Direct-Fed Microbial Dosage on the Fecal Concentrations of Enterohemorrhagic Escherichia coli in Feedlot Cattle SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID LACTOBACILLUS-ACIDOPHILUS; O157; FECES; PREVALENCE; GENE; CONTAMINATION; PERFORMANCE; STRATEGY; CULTURE; VACCINE AB Contamination of beef products by Shiga toxin-producing Escherichia coli is a concern for food safety with a particular subset, the enterohemorrhagic E. coli (EHEC), being the most relevant to human disease. To mitigate food safety risks, preharvest intervention strategies have been implemented with the aim to reduce EHEC in cattle. One class of interventions that has been widely used in feedlots is direct-fed microbials (DFMs), which can contain various dosing rates of probiotic bacteria. Here we compare the use of two different doses of a commercially available DFM on total EHEC load in a commercial feedlot setting. The DFMs used were the standard 10(9)Propionibacterium freudenreichii and 10(6)Lactobacillus acidophilus colony forming units (CFUs)/head/day dose of Bovamine((R)) (Nutrition Physiology Company, Guymon, OK) and the higher dose, Bovamine Defend (Nutrition Physiology Company), which is dosed at 10(9)P. freudenreichii and 10(9)Lactobacillus acidophilus CFUs/head/day. To analyze the total EHEC fecal concentration, 2200 head of cattle were assigned a DFM feed regimen lasting approximately 5 months. At harvest, 480 head of cattle were sampled using rectoanal mucosal swabs. A quantitative polymerase chain reaction assay targeting ecf1 was used to enumerate the total EHEC fecal concentration for 240 head fed the low-dose DFM and 240 head fed the high-dose DFM. No significant difference (p>0.05) in the fecal concentration of total EHEC was observed between the two doses. This suggests that using an increased dosage provides no additional reduction in the total EHEC fecal concentration of feedlot cattle compared to the standard dosage. C1 [Luedtke, Brandon E.; Bosilevac, Joseph M.; Harhay, Dayna M.; Arthur, Terrance M.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, State Spur 18D, Clay Ctr, NE 68933 USA. [Luedtke, Brandon E.] Univ Nebraska Kearney, Dept Biol, Kearney, NE USA. RP Arthur, TM (reprint author), USDA ARS, Roman L Hruska US Meat Anim Res Ctr, State Spur 18D, Clay Ctr, NE 68933 USA. EM terrance.arthur@ars.usda.gov NR 35 TC 0 Z9 0 U1 4 U2 10 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD APR PY 2016 VL 13 IS 4 BP 190 EP 195 DI 10.1089/fpd.2015.2063 PG 6 WC Food Science & Technology SC Food Science & Technology GA DK1CB UT WOS:000374648900004 PM 26974651 ER PT J AU Rodriguez-Rivera, LD Cummings, KJ Loneragan, GH Rankin, SC Hanson, DL Leone, WM Edrington, TS AF Rodriguez-Rivera, Lorraine D. Cummings, Kevin J. Loneragan, Guy H. Rankin, Shelley C. Hanson, Devin L. Leone, William M. Edrington, Thomas S. TI Salmonella Prevalence and Antimicrobial Susceptibility Among Dairy Farm Environmental Samples Collected in Texas SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID DRUG-RESISTANT SALMONELLA; ENTERICA SEROVAR NEWPORT; ACTIVE SURVEILLANCE NETWORK; NON-TYPHOIDAL SALMONELLA; 10 US SITES; UNITED-STATES; ESCHERICHIA-COLI; EXTRAINTESTINAL INFECTION; NONTYPHOIDAL SALMONELLA; FEEDLOT CATTLE AB Dairy cattle are a reservoir of several Salmonella serovars that are leading causes of human salmonellosis. The objectives of this study were to estimate the environmental prevalence of Salmonella on dairy farms in Texas and to characterize the antimicrobial susceptibility of the isolates. Eleven dairy farms throughout Texas were sampled from August through October 2013, using a cross-sectional approach. Samples were collected from four locations within each farm (hospital pen, maternity pen, cow housing area, and calf housing area), and feces were collected from cull cows as available. Environmental and fecal samples were processed for Salmonella, and isolates were tested for susceptibility to 15 antimicrobial agents. Serovar characterization was performed on a subset of these isolates. Salmonella was isolated from 67.0% (236/352) of the environmental samples and 64.2% (43/67) of the cull cow fecal samples. Environmental samples from the maternity pen were significantly more likely to be Salmonella positive than samples from the cow and calf housing areas. Multidrug resistance was evident in 11.9% (27/226) of environmental isolates and 19.5% (8/41) of fecal isolates. Salmonella isolates from the calf housing area and maternity pen were significantly more likely to be multidrug resistant (MDR) than isolates from the cow housing area. The most common serovars found among the MDR isolates were Newport, Muenchen, and Typhimurium. These results help provide a focus for efforts to mitigate the burden of antimicrobial-resistant Salmonella at the preharvest level. C1 [Rodriguez-Rivera, Lorraine D.; Cummings, Kevin J.] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Integrat Biosci, College Stn, TX 77843 USA. [Loneragan, Guy H.; Hanson, Devin L.] Texas Tech Univ, Int Ctr Food Ind Excellence, Lubbock, TX 79409 USA. [Rankin, Shelley C.] Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA USA. [Edrington, Thomas S.] USDA ARS, Food & Feed Safety Res Unit, College Stn, TX USA. RP Cummings, KJ (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Integrat Biosci, College Stn, TX 77843 USA. EM kcummings@cvm.tamu.edu FU Beef Checkoff; U.S. Department of Agriculture National Institute of Food and Agriculture's National Integrated Food Safety Initiative [2011-51110-31081] FX The authors thank the dairy farm owners who participated in this study as well as Kate Andrews for her excellent technical assistance. This research was funded by The Beef Checkoff and the U.S. Department of Agriculture National Institute of Food and Agriculture's National Integrated Food Safety Initiative award no. 2011-51110-31081. NR 56 TC 0 Z9 0 U1 6 U2 11 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD APR PY 2016 VL 13 IS 4 BP 205 EP 211 DI 10.1089/fpd.2015.2037 PG 7 WC Food Science & Technology SC Food Science & Technology GA DK1CB UT WOS:000374648900006 PM 26954516 ER PT J AU Saha, BC Qureshi, N Kennedy, GJ Cotta, MA AF Saha, Badal C. Qureshi, Nasib Kennedy, Gregory J. Cotta, Michael A. TI Biological pretreatment of corn stover with white-rot fungus for improved enzymatic hydrolysis SO INTERNATIONAL BIODETERIORATION & BIODEGRADATION LA English DT Article DE White-rot fungus; Biological pretreatment; Enzymatic hydrolysis; Solid state cultivation; Corn stover ID PHANEROCHAETE-CHRYSOSPORIUM; RICE STRAW; LIGNOCELLULOSIC BIOMASS; ACID PRETREATMENT; WHEAT-STRAW; SACCHARIFICATION; ETHANOL; LIGNIN; FERMENTATION; WOOD AB Biological pretreatment of lignocellulosic biomass by white-rot fungus can represent a low-cost and eco-friendly alternative to harsh physical, chemical or physico-chemical pretreatment methods to facilitate enzymatic hydrolysis. However, fungal pretreatment can cause carbohydrate loss and it is, therefore, necessary to use the appropriate fungal strain-biomass type combination. In this work, 26 white-rot fungal strains were evaluated under solid state cultivation at 74% moisture level at 28 degrees C for 30 days for fermentable sugar production from corn stover after enzymatic hydrolysis of each fungal pretreated corn stover using a cocktail of 3 commercial enzyme (cellulase, beta-glucosidase, hemicellulase) preparations. The best result was obtained with Cyathus stercoreus NRRL-6573 which gave a sugar yield of 394 +/- 13 mg/g of pretreated stover followed by Pycnoporus sanguineus FP-10356-Sp (393 +/- 17 mg/g) and Phlebia brevispora NRRL-13108 (383 +/- 13 mg/g). Published by Elsevier Ltd. C1 [Saha, Badal C.; Qureshi, Nasib; Kennedy, Gregory J.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Saha, BC (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Badal.Saha@ars.usda.gov NR 33 TC 6 Z9 6 U1 10 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0964-8305 EI 1879-0208 J9 INT BIODETER BIODEGR JI Int. Biodeterior. Biodegrad. PD APR PY 2016 VL 109 BP 29 EP 35 DI 10.1016/j.ibiod.2015.12.020 PG 7 WC Biotechnology & Applied Microbiology; Environmental Sciences SC Biotechnology & Applied Microbiology; Environmental Sciences & Ecology GA DK0OE UT WOS:000374611300004 ER PT J AU Stentiford, GD Becnel, JJ Weiss, LM Keeling, PJ Didier, ES Williams, BAP Bjornson, S Kent, ML Freeman, MA Brown, MJF Troemel, ER Roesel, K Sokolova, Y Snowden, KF Solter, L AF Stentiford, G. D. Becnel, J. J. Weiss, L. M. Keeling, P. J. Didier, E. S. Williams, B. A. P. Bjornson, S. Kent, M. L. Freeman, M. A. Brown, M. J. F. Troemel, E. R. Roesel, K. Sokolova, Y. Snowden, K. F. Solter, L. TI Microsporidia - Emergent Pathogens in the Global Food Chain SO TRENDS IN PARASITOLOGY LA English DT Review ID MUSCIDIFURAX-RAPTOR HYMENOPTERA; HUMAN-IMMUNODEFICIENCY-VIRUS; BONE-MARROW-TRANSPLANTATION; COLONY COLLAPSE DISORDER; ENTEROCYTOZOON-BIENEUSI; N-SP; ENCEPHALITOZOON-CUNICULI; INTESTINAL MICROSPORIDIOSIS; AIDS PATIENTS; BUMBLE BEES AB Intensification of food production has the potential to drive increased disease prevalence in food plants and animals. Microsporidia are diversely distributed, opportunistic, and density-dependent parasites infecting hosts from almost all known animal taxa. They are frequent in highly managed aquatic and terrestrial hosts, many of which are vulnerable to epizootics, and all of which are crucial for the stability of the animal-human food chain. Mass rearing and changes in global climate may exacerbate disease and more efficient transmission of parasites in stressed or immune-deficient hosts. Further, human microsporidiosis appears to be adventitious and primarily associated with an increasing community of immune-deficient individuals. Taken together, strong evidence exists for an increasing prevalence of microsporidiosis in animals and humans, and for sharing of pathogens across hosts and biomes. C1 [Stentiford, G. D.] CEFAS, Pathol & Mol Systemat Team, Barrack Rd, Weymouth DT4 8UB, Dorset, England. [Becnel, J. J.] ARS, USDA, CMAVE, 1600 South West 23rd Dr, Gainesville, FL 32608 USA. [Weiss, L. M.] Albert Einstein Coll Med, 1300 Morris Pk Ave,Forchheimer 504, Bronx, NY 10641 USA. [Keeling, P. J.] Univ British Columbia, Dept Bot, Canadian Inst Adv Res, 3529-6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada. [Didier, E. S.] Tulane Univ, Div Microbiol, Tulane Natl Primate Res Ctr, 1440 Canal St, New Orleans, LA 70112 USA. [Didier, E. S.] Tulane Univ, Dept Trop Med, Sch Publ Hlth & Trop Med, 1440 Canal St, New Orleans, LA 70112 USA. [Williams, B. A. P.] Univ Exeter, Biosci, Coll Life & Environm Sci, Stocker Rd, Exeter EX4 4QD, Devon, England. [Bjornson, S.] St Marys Univ, Dept Biol, 923 Robie St, Halifax, NS B3H 3C3, Canada. [Kent, M. L.] Oregon State Univ, Dept Microbiol, 220 Nash Hall, Corvallis, OR 97331 USA. [Kent, M. L.] Oregon State Univ, Dept Biomed Sci, 220 Nash Hall, Corvallis, OR 97331 USA. [Freeman, M. A.] Ross Univ, Sch Vet Med, St Kitts, West Indies, St Kitts & Nevi. [Brown, M. J. F.] Royal Holloway Univ London, Sch Biol Sci, Egham TW20 0EX, Surrey, England. [Troemel, E. R.] Univ Calif San Diego, 4202 Bonner Hall,9500 Gilman Dr 0349, La Jolla, CA 92093 USA. [Roesel, K.] Free Univ Berlin, Int Livestock Res Inst, Inst Parasitol & Trop Vet Med, Robert von Ostertag Str 7-13, D-14163 Berlin, Germany. [Sokolova, Y.] Louisiana State Univ, Dept Comparat Biomed Sci, Sch Vet Med, 1909 Skip Beaman Dr, Baton Rouge, LA 70803 USA. [Snowden, K. F.] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, Mailstop 4467, College Stn, TX 77843 USA. [Solter, L.] Univ Illinois, Illinois Nat Hist Survey, Prairie Res Inst, 1816 South Oak St, Champaign, IL 61820 USA. RP Solter, L (reprint author), Univ Illinois, Illinois Nat Hist Survey, Prairie Res Inst, 1816 South Oak St, Champaign, IL 61820 USA. EM lsolter@illinois.edu OI Williams, Bryony/0000-0003-1123-2176; Roesel, Kristina/0000-0002-2553-1129 FU DG SANCO of the European Commission [C5473]; UK Department for Environment, Food, and Rural Affairs (DEFRA) [FB002] FX This review is an output from a symposium sponsored by the Organisation for Economic Cooperation and Development (OECD) Cooperative Research Programme (CRP) on Biological Resource Management for Sustainable Agricultural Systems and the Society for Invertebrate Pathology (SIP), held on 9th August 2015 at the University of British Columbia, Vancouver, BC, Canada. The symposium was entitled 'Microsporidia in the Animal to Human Food Chain: An International Symposium To Address Chronic Epizootic Disease'. We acknowledge the generous funding provided by the OECD CRP and the SIP to speakers at this event. The lead author (G.D.S.) would like to acknowledge funding by DG SANCO of the European Commission (under contract C5473) and the UK Department for Environment, Food, and Rural Affairs (DEFRA) (under contract FB002). NR 114 TC 11 Z9 11 U1 12 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1471-4922 EI 1471-5007 J9 TRENDS PARASITOL JI Trends Parasitol. PD APR PY 2016 VL 32 IS 4 BP 336 EP 348 DI 10.1016/j.pt.2015.12.004 PG 13 WC Parasitology SC Parasitology GA DK0NT UT WOS:000374610200010 PM 26796229 ER PT J AU Zhao, LS Huang, CH Wu, FQ AF Zhao, Longshan Huang, Chihua Wu, Faqi TI Effect of microrelief on water erosion and their changes during rainfall SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE digital elevation model; microrelief; runoff and erosion; soil erosion; soil surface roughness ID SOIL SURFACE-ROUGHNESS; PARTICLE-SIZE DISTRIBUTION; OVERLAND-FLOW; DEPRESSIONAL STORAGE; SIMULATED RAINFALL; ERODED SEDIMENT; INFILTRATION; INTERRILL; TILLAGE; RUNOFF AB Soil surface roughness contains two elementary forms, depressions and mounds, which affect water flow on the surface differently. While depressions serve as temporary water storage, mounds divert water away from their local summits. Although roughness impacts on runoff and sediment production have been studied, almost no studies have been designed explicitly to quantify the evolution of depressions and mounds and how this impacts runoff generation and sediment delivery. The objectives of this study were to analyze how different surface forms affect runoff and sediment delivery and to measure the changes in surface depressions and mounds during rainfall events. A smooth surface was used as the control. Both mounds and depressions delayed the runoff initiating time, but to differing degrees; and slightly reduced surface runoff when compared to the runoff process from the smooth surface. Surface mounds significantly increased sediment delivery, whilst depressions provided surface storage and hence reduced sediment delivery. However, as rainfall continued and rainfall intensity increased, the depression effect on runoff and erosion gradually decreased and produced even higher sediment delivery than the smooth surface. Depressions and mounds also impacted the particle size distribution of the discharged sediments. Many more sand-sized particles were transported from the surface with mounds than with depressions. The morphology of mounds and depressions changed significantly due to rainfall, but to different extents. The difference in change had a spatial scale effect, i.e. erosion from each mound contributed to its own morphological change while sediments deposited in a depression came from a runoff contributing area above the depression, hence a much greater source area than a single mound. The results provide a mechanistic understanding of how soil roughness affects runoff and sediment production. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Zhao, Longshan] Guizhou Univ, Coll Forestry, Guiyang 550003, Guizhou, Peoples R China. [Zhao, Longshan; Wu, Faqi] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling, Shaanxi, Peoples R China. [Zhao, Longshan; Huang, Chihua] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Zhao, LS (reprint author), Guizhou Univ, Coll Forestry, Guiyang 550003, Guizhou, Peoples R China. EM longshanzh@163.com FU China Scholarship Council (CSC); National Natural Science Foundation of China [41271288] FX L. Zhao would like to acknowledge the scholarship from China Scholarship Council (CSC). This study was funded by the National Natural Science Foundation of China (No. 41271288). Two anonymous reviewers, Professor Lane and the journal's editors are acknowledged for their comments of the manuscript. NR 41 TC 2 Z9 3 U1 5 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD APR PY 2016 VL 41 IS 5 BP 579 EP 586 DI 10.1002/esp.3844 PG 8 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DJ1XO UT WOS:000373998500001 ER PT J AU Stote, KS Tracy, RP Taylor, PR Baer, DJ AF Stote, K. S. Tracy, R. P. Taylor, P. R. Baer, D. J. TI The effect of moderate alcohol consumption on biomarkers of inflammation and hemostatic factors in postmenopausal women SO EUROPEAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID C-REACTIVE PROTEIN; PLASMINOGEN-ACTIVATOR INHIBITOR-1; CARDIOVASCULAR RISK-FACTORS; CORONARY-HEART-DISEASE; RED WINE; MYOCARDIAL-INFARCTION; RANDOMIZED CROSSOVER; ADHESION MOLECULES; CONTROLLED DIET; FACTOR-VII AB BACKGROUND/OBJECTIVES: Inflammation and hemostasis contribute to the etiology of cardiovascular disease. We previously demonstrated that moderate alcohol consumption (1-2 drinks/day) may decrease risk for cardiovascular disease because of an improved lipid profile. In addition to these beneficial changes, the alcohol-mediated reduction in risk may be through its effect on inflammation and hemostasis. The objective of the study was to evaluate the effect of moderate alcohol consumption on biomarkers of inflammation and hemostasis in postmenopausal women. SUBJECTS/METHODS: As part of a controlled diet study, 53 postmenopausal women each consumed a weight-maintaining diet plus 0, 15 and 30 g/day of alcohol for 8 weeks, in a randomized crossover design. The controlled diet contained 15%, 53% and 32% of energy from protein, carbohydrate and fat, respectively. RESULTS: Soluble intercellular adhesion molecule-1 decreased by 5% (P<0.05) with consumption of both 15 and 30 g of alcohol. Fibrinogen concentrations decreased by 4% and 6% (P<0.05) after consumption of 15 and 30 g alcohol, respectively. Fibrin D-dimer decreased by 24% (P<0.05) after consumption of 30 g of alcohol. Plasminogen activator inhibitor-1 (PAI-1) concentrations were increased 27 and 54% (P<0.05) after consumption of 15 and 30 g of alcohol. Plasma high-sensitivity C-reactive protein, interleukin-6 and factor VII coagulant activity did not change with alcohol consumption. CONCLUSIONS: These data suggest that moderate alcohol consumption may have beneficial effects on inflammation and hemostasis in postmenopausal women, and this may be somewhat mitigated by an increase in PAI-1. C1 [Stote, K. S.; Baer, D. J.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. [Tracy, R. P.] Univ Vermont, Coll Med, Burlington, VT USA. [Taylor, P. R.] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. SUNY Empire State Coll, Saratoga Springs, NY 12866 USA. RP Stote, KS (reprint author), SUNY Empire State Coll, 113 West Ave, Saratoga Springs, NY 12866 USA. EM Kim.Stote@esc.edu NR 41 TC 2 Z9 2 U1 2 U2 7 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0954-3007 EI 1476-5640 J9 EUR J CLIN NUTR JI Eur. J. Clin. Nutr. PD APR PY 2016 VL 70 IS 4 BP 470 EP 474 DI 10.1038/ejcn.2015.182 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DJ3RO UT WOS:000374123500010 PM 26554758 ER PT J AU McKeown, NM Marklund, M Ma, JT Ross, AB Lichtenstein, AH Livingston, KA Jacques, PF Rasmussen, HM Blumberg, JB Chen, CYO AF McKeown, Nicola M. Marklund, Matti Ma, Jiantao Ross, Alastair B. Lichtenstein, Alice H. Livingston, Kara A. Jacques, Paul F. Rasmussen, Helen M. Blumberg, Jeffrey B. Chen, C-Y. Oliver TI Comparison of plasma alkylresorcinols (AR) and urinary AR metabolites as biomarkers of compliance in a short-term, whole-grain intervention study SO EUROPEAN JOURNAL OF NUTRITION LA English DT Article DE Alkylresorcinols; Biomarkers; Whole grain; Whole wheat ID LIVING SWEDISH ADULTS; CEREAL FIBER INTAKE; RYE INTAKE; ELECTROCHEMICAL DETECTION; POTENTIAL BIOMARKERS; POSTMENOPAUSAL WOMEN; FINNISH WOMEN; WHEAT; PRODUCTS; REPRODUCIBILITY AB Alkylresorcinols (AR) are phenolic lipids present in the bran of wheat and rye. Plasma AR and their urinary metabolites may be suitable biomarkers of whole-grain (WG) wheat and rye consumption. The objective of this study was to examine plasma AR and urinary AR metabolites in response to WG wheat consumption. In a randomized crossover study, 19 subjects (10 males, 9 females; BMI 22.0 kg/m(2); age 26 years) incorporated either 3 servings (48 g) or 6 servings (96 g) of WG wheat daily into their regular diet for 1 week. Subjects completed a 2-week washout period, abstaining from all WG consumption, before each intervention. Fasting blood and 24-h urine were collected before and after each intervention. Plasma AR homologues (C19:0, C21:0, C23:0) were quantified by GC-MS after diethyl ether and solid phase extraction and derivatization. Urinary AR metabolites [3,5-dihydroxybenzoic acid and 3-(3,5-dihydroxyphenyl)-propanoic acid] were determined using HPLC with electrochemical detection after enzymatic deconjugation and ethyl acetate extraction. Urinary total AR metabolites were significantly higher after 6 compared with 3 servings of WG wheat (56 vs. 32 mu mol/day, P < 0.001). This dose-response relationship was independent of age, sex, energy intake, and baseline urinary AR metabolite concentration. Plasma total AR tended to be higher after 6 compared with 3 servings of WG wheat (103.0 vs. 86.9 nmol/L), but this difference was not significant (P = 0.42). The results suggest that urinary AR metabolites from 24-h urine collections may be useful as biomarkers of compliance in intervention studies of WG wheat. C1 [McKeown, Nicola M.; Ma, Jiantao; Lichtenstein, Alice H.; Livingston, Kara A.; Jacques, Paul F.; Rasmussen, Helen M.; Blumberg, Jeffrey B.; Chen, C-Y. Oliver] Tufts Univ, Jean Mayer US Dept Agr USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. [McKeown, Nicola M.; Lichtenstein, Alice H.; Jacques, Paul F.; Rasmussen, Helen M.; Blumberg, Jeffrey B.; Chen, C-Y. Oliver] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Marklund, Matti] Uppsala Univ, Dept Publ Hlth & Caring Sci Clin Nutr & Metab, Uppsala, Sweden. [Ross, Alastair B.] Chalmers, Dept Life Sci Engn, Food & Nutr Sci, S-41296 Gothenburg, Sweden. RP McKeown, NM (reprint author), Tufts Univ, Jean Mayer US Dept Agr USDA, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA.; McKeown, NM (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu OI Ross, Alastair/0000-0002-9585-0141; Marklund, Matti/0000-0002-3320-796X FU General Mills Bell Institute of Health and Nutrition; USDA Agricultural Research Service [58-1950-0-014] FX The authors' responsibilities were as follows: NMM, AHL, and PFJ contributed to the study concept and analysis design; ABR, MM, KAL, JBB, and CYC contributed to data acquisition; JM and MM performed the statistical analysis; MM, CYC, and NMM contributed to the interpretation of data; JBB and CYC conducted the blood AR analysis, and MM conducted the analysis of urinary AR metabolites; ABR analyzed the AR in the food; HR designed the metabolic diets; NMM, MM, and KAL drafted the manuscript, and all authors were involved in critical revision and had final approval of the manuscript. We thank Dustin Burnett for his assistance in the initial design of the metabolic diet and Dr. Robert Moreau for initial analysis of the AR content of the food. The authors also acknowledge the cooperation of study participants and the Metabolic Research Unit and Nutrition Evaluation Laboratory staff at the Human Nutrition Research Center on Aging at Tufts University. This study was supported by grants from the General Mills Bell Institute of Health and Nutrition and a grant from the USDA Agricultural Research Service (agreement 58-1950-0-014). NR 38 TC 2 Z9 2 U1 4 U2 6 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1436-6207 EI 1436-6215 J9 EUR J NUTR JI Eur. J. Nutr. PD APR PY 2016 VL 55 IS 3 BP 1235 EP 1244 DI 10.1007/s00394-015-0936-8 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DJ3NS UT WOS:000374112900032 PM 26043861 ER PT J AU Isselhardt, ML Perkins, TD van den Berg, AK Schaberg, PG AF Isselhardt, Mark L. Perkins, Timothy D. van den Berg, Abby K. Schaberg, Paul G. TI Preliminary Results of Sugar Maple Carbohydrate and Growth Response under Vacuum and Gravity Sap Extraction SO FOREST SCIENCE LA English DT Article DE maple syrup; nonstructural carbohydrate (NSC); maple sap; growth ID ACER-SACCHARUM; TREES; RESERVES; DECLINE; AGE AB Recent technological advancements have increased the amount of sugar-enriched sap that can be extracted from sugar maple (Acer saccharum). This pilot study quantified overall sugar removal and the impacts of vacuum (60 cm Hg) and gravity sap extraction on residual nonstructural carbohydrate (NSC) concentrations and on stem and twig growth. Vacuum sap extraction (VSE) resulted in significantly greater mean sugar removal (1.19 +/- 0.46 kg [SE]) than gravity sap extraction (GSE) (0.48 +/- 0.14 kg). Residual stem NSC displayed a pattern of increased concentration with increased extraction. Twig residual NSC concentrations were highly variable, perhaps because of the highly dynamic late spring period, and no clear patterns were observed. Mean radial stem growth in the year after sap extraction was greater in untapped trees (2.93 +/- 0.58 mm) than with VSE (1.99 +/- 0.44 mm) or GSE (1.67 +/- 0.12 mm). The results raise the possibility that sap removal shifts sugar maple NSC source-sink relationships toward storage at the expense of growth. C1 [Isselhardt, Mark L.] Univ Vermont, Plant Biol, Proctor Maple Res Ctr, Underhill Ctr, Burlington, VT 05405 USA. [Perkins, Timothy D.; van den Berg, Abby K.] Univ Vermont, Proctor Maple Res Ctr, Burlington, VT 05405 USA. [Schaberg, Paul G.] US Forest Serv, USDA, Northern Res Stn, Washington, DC 20250 USA. RP Isselhardt, ML (reprint author), Univ Vermont, Plant Biol, Proctor Maple Res Ctr, Underhill Ctr, Burlington, VT 05405 USA.; Perkins, TD; van den Berg, AK (reprint author), Univ Vermont, Proctor Maple Res Ctr, Burlington, VT 05405 USA.; Schaberg, PG (reprint author), US Forest Serv, USDA, Northern Res Stn, Washington, DC 20250 USA. EM mark.isselhardt@uvm.edu; timothy.perkins@uvm.edu; avan@uvm.edu; pschaberg@fs.fed.us FU University of Vermont, Agricultural Experiment Station FX We thank Brian Stowe (University of Vermont, Proctor Maple Research Center) and Paula Murakami (USDA Forest Service) for their technical assistance in the field and laboratory, respectively. This research was supported by fiends provided by the University of Vermont, Agricultural Experiment Station. NR 14 TC 0 Z9 0 U1 8 U2 15 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2016 VL 62 IS 2 BP 125 EP 128 DI 10.5849/forsci.14-137 PG 4 WC Forestry SC Forestry GA DJ5VA UT WOS:000374277000001 ER PT J AU Clarke, SR Riggins, JJ Stephen, FM AF Clarke, Stephen R. Riggins, John J. Stephen, Frederick M. TI Forest Management and Southern Pine Beetle Outbreaks: A Historical Perspective SO FOREST SCIENCE LA English DT Article DE southern pine beetle; forest management; population dynamics; suppression ID DENDROCTONUS-FRONTALIS COLEOPTERA; BARK BEETLES; EAST TEXAS; OLEORESIN CHARACTERISTICS; ZIMMERMAN COLEOPTERA; POPULATION-DYNAMICS; FLIGHT ACTIVITY; UNITED-STATES; LOBLOLLY-PINE; LONGLEAF PINE AB The southern pine beetle (Dendroctonus frontalis) (SPB) is an eruptive pest of pine forests in the southeastern United States. Numerous studies have been conducted on the relationships among SPB population dynamics, climatic factors, natural enemies, and competitors, but the influence of changes in forest management through time on SPB activity has received little attention. Forest management dictates the configuration and condition of hosts available for SPB populations, whereas suppression has an impact on population levels of SPB and their associates and also affects the area and distribution of the susceptible host type remaining after treatment. In contrast to the frequent and widespread SPB outbreaks in the last half of the 20th century, recent SPB activity in the Southeast has been localized and short-lived. Reports from the mid-1800s through the mid-1900s indicate that outbreaks then were also less common. In this review, we examine how changes in forest management practices have played a significant role in the history of SPB outbreaks. C1 [Clarke, Stephen R.] US Forest Serv, USDA, Forest Hlth Protect, Lufkin, TX 75904 USA. [Riggins, John J.] Mississippi State Univ, Mississippi State, MS 39762 USA. [Stephen, Frederick M.] Univ Arkansas, Fayetteville, AR 72701 USA. RP Clarke, SR (reprint author), US Forest Serv, USDA, Forest Hlth Protect, Lufkin, TX 75904 USA.; Riggins, JJ (reprint author), Mississippi State Univ, Mississippi State, MS 39762 USA.; Stephen, FM (reprint author), Univ Arkansas, Fayetteville, AR 72701 USA. EM sclarke@fs.fed.us; jriggins@entomology.msstate.edu; fstephen@uark.edu NR 187 TC 0 Z9 0 U1 6 U2 17 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2016 VL 62 IS 2 BP 166 EP 180 DI 10.5849/forsci.15-071 PG 15 WC Forestry SC Forestry GA DJ5VA UT WOS:000374277000005 ER PT J AU Hrinkevich, KH Progar, RA Shaw, DC AF Hrinkevich, Kathryn H. Progar, Robert A. Shaw, David C. TI A Severity Rating System for Evaluating Stand-Level Balsam Woolly Adelgid (Hemiptera: Adelgidae) Damage in Two Abies Species in Western North America SO FOREST SCIENCE LA English DT Article DE balsam woolly adelgid; invasive insect; nonnative; forest impact; high elevation ID CAROLINA; APHID AB Severity rating systems are fundamental to understanding the impacts of disturbance agents in forest stands. The balsam woolly adelgid (BWA), Adelges piceae (Raizeburg) (Hemiptera: Adelgidae), is an invasive forest pest in North America that infests and causes mortality in true fir, Abies spp. There is currently no single system for evaluating damage caused by BWA in the western United States. Because range expansion through favored habitat is inevitable, it is imperative to begin long-term monitoring using a unified approach to evaluate changing conditions and hasten management opportunities. We developed a new rating system for two western host species: grand fir and subalpine fir. Unlike other severity scales, our index describes stand-level damage rather than impacts on individual host trees alone. We sampled 57 sites across the current range of BWA in the western United States and compiled severity indices using 10 metrics of overstory and understory damage. We used analyses of variance to identify five discrete severity classes and translated the results into a descriptive table of damage characteristics for each class. This index is proposed as an improvement over existing rating systems for western North America because of its broader scope, demonstrated ability to distinguish between classes, and identification of the predominant indicators that will improve the efficiency and efficacy of field assessments. The adoption of this system will facilitate long-term monitoring through site resurveys that will be directly comparable over time, also allowing future studies to conduct risk assessments and target stands that face the greatest threat to forest health. C1 [Hrinkevich, Kathryn H.] Oregon State Univ, Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Progar, Robert A.] US Forest Serv, USDA, Washington, DC 20250 USA. [Shaw, David C.] Oregon State Univ, Corvallis, OR 97331 USA. RP Hrinkevich, KH (reprint author), Oregon State Univ, Forest Engn Resources & Management, Corvallis, OR 97331 USA.; Progar, RA (reprint author), US Forest Serv, USDA, Washington, DC 20250 USA.; Shaw, DC (reprint author), Oregon State Univ, Corvallis, OR 97331 USA. EM hrinky@gmail.com; rprogar@fs.fed.us; dave.shaw@oregonstate.edu FU USDA Forest Service State and Private Forestry Special Technology Development Program; Forest Health Protection FX This project was funded by the USDA Forest Service State and Private Forestry Special Technology Development Program and Forest Health Protection, which also helped with site selection and background knowledge. Special thanks are owed to Fred Hain, Robert Jeeton, Doug Westlind, Lia Spei gel, and Tim Harrington for very helpful reviews. NR 36 TC 0 Z9 0 U1 3 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2016 VL 62 IS 2 BP 181 EP 189 DI 10.5849/forsci.15-025 PG 9 WC Forestry SC Forestry GA DJ5VA UT WOS:000374277000006 ER PT J AU Jackson, MB Hayes, CJ Taylor, JE Ferguson, BA AF Jackson, Marcus B. Hayes, Christopher J. Taylor, Jane E. Ferguson, Brennan A. TI Effects of Thinning and Overstory Removal on Western Larch and Western Larch Dwarf Mistletoe SO FOREST SCIENCE LA English DT Article DE dwarf mistletoe; larch; thinning; spread and intensification; Arceuthobium laricis; uneven-age management; overstory removal ID INTENSIFICATION; HEMLOCK; SPREAD; FIR AB Western larch dwarf mistletoe (Arceuthobium lauds) causes an important disease of western larch (Larix occidentalis), resulting in growth loss and mortality. Little is known about the spread, intensification, and impact of this parasitic plant on its principal host, western larch. We examined the impact of precommercial thinning and (or) overstory removal on western larch crop tree height and diameter growth, as well as spread and intensification of western larch dwarf mistletoe over 21 years in a western Montana larch stand. In addition, upward advancement of dwarf mistletoe in crop tree crowns and dwarf mistletoe effects on mortality in both overstory and understory trees were explored. Overstory tree mortality was measured 15 years after treatment, and understory trees were measured 5 times over 21 years. Mulitivariate linear models showed that initial crop tree size and treatment were predictors of stem diameter 21 years after treatment. Models also showed that the initial dwarf mistletoe rating (DMR) of crop trees and treatment were reliable predictors of the changes in DMR after each treatment, but treatment did not predict the dwarf mistletoe index. Dwarf mistletoe upward advancement in larch crowns was greater than the rate of tree height growth in all treatments. Of the larch crop trees that died in the 21 years after treatment, western larch dwarf mistletoe was implicated in about 35% of the mortality. Eight percent of overstory larch rated as DMR 5 or 6 at study establishment were dead after 15 years. Although mean DMRs for crop trees were low at study establishment (average DMR <1), treatments were probably implemented about 15 years later than needed to best demonstrate differences in treatment impacts. Management implications are discussed. C1 [Jackson, Marcus B.] US Forest Serv, USDA, Forest Hlth Protect, Missoula, MT 59807 USA. [Hayes, Christopher J.; Taylor, Jane E.; Ferguson, Brennan A.] US Forest Serv, USDA, Washington, DC 20250 USA. RP Jackson, MB (reprint author), US Forest Serv, USDA, Forest Hlth Protect, Missoula, MT 59807 USA.; Hayes, CJ; Taylor, JE; Ferguson, BA (reprint author), US Forest Serv, USDA, Washington, DC 20250 USA. EM mbjackson@fs.fed.us; cjhayes@fs.fed.us; jetaylor@fs.fed.us; brennanferguson@fs.fed.us NR 34 TC 0 Z9 0 U1 4 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2016 VL 62 IS 2 BP 190 EP 199 DI 10.5849/forsci.15-073 PG 10 WC Forestry SC Forestry GA DJ5VA UT WOS:000374277000007 ER PT J AU da Graca, JV Douhan, GW Halbert, SE Keremane, ML Lee, RF Vidalakis, G Zhao, HW AF da Graca, John V. Douhan, Greg W. Halbert, Susan E. Keremane, Manjunath L. Lee, Richard F. Vidalakis, Georgios Zhao, Hongwei TI Huanglongbing: An overview of a complex pathosystem ravaging the world's citrus SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Review DE Citrus greening; Diaphorina citri; host response; Huanglongbing (HLB); psyllid vectors ID CANDIDATUS LIBERIBACTER-ASIATICUS; COMPLETE GENOME SEQUENCE; BACTERICERA-NIGRICORNIS FORSTER; GREENING DISEASE; DIAPHORINA-CITRI; ASIAN CITRUS; 1ST REPORT; HEMIPTERA PSYLLIDAE; GENETIC DIVERSITY; TRIOZA-ERYTREAE AB Citrus huanglongbing (HLB) has become a major disease and limiting factor of production in citrus areas that have become infected. The destruction to the affected citrus industries has resulted in a tremendous increase to support research that in return has resulted in significant information on both applied and basic knowledge concerning this important disease to the global citrus industry. Recent research indicates the relationship between citrus and the causal agent of HLB is shaped by multiple elements, in which host defense responses may also play an important role. This review is intended to provide an overview of the importance of HLB to a wider audience of plant biologists. Recent advances on host-pathogen interactions, population genetics and vectoring of the causal agent are discussed. C1 [da Graca, John V.] Texas A&M Univ, Kingsville Citrus Ctr, Weslaco, TX 78599 USA. [Douhan, Greg W.; Vidalakis, Georgios] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Halbert, Susan E.] Florida Dept Agr & Consumer Serv, Div Plant Ind, POB 147100, Gainesville, FL 32614 USA. [Keremane, Manjunath L.; Lee, Richard F.] USDA ARS, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA 92507 USA. [Zhao, Hongwei] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China. RP Vidalakis, G (reprint author), Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA.; Zhao, HW (reprint author), Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China. EM georgrios.vidalakis@ucr.edu; hzhao@njau.edu.cn NR 125 TC 3 Z9 3 U1 24 U2 56 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD APR PY 2016 VL 58 IS 4 SI SI BP 373 EP 387 DI 10.1111/jipb.12437 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DJ3XL UT WOS:000374139900009 PM 26466921 ER PT J AU Perkins, LB Hatfield, G Espeland, EK AF Perkins, Lora B. Hatfield, Gary Espeland, Erin K. TI Invasive grasses consistently create similar plant-soil feedback types in soils collected from geographically distant locations SO JOURNAL OF PLANT ECOLOGY LA English DT Article DE plant-soil feedback; invasion; niche construction ID CENTAUREA-SOLSTITIALIS; MICROBIAL COMMUNITIES; BIOTA; METAANALYSIS; DIVERSITY; CONSEQUENCES; BIODIVERSITY; ALLELOPATHY; ENGINEERS; DYNAMICS AB Aims Plants of similar life forms and closely related species have been observed to create similar types of plant-soil feedbacks (PSFs). However, investigations of the consistency of PSFs within species have not yielded clear results. For example, it has been reported that species create different types of PSFs in their native and introduced ranges. The aim of this project is to examine if four species create similar PSF types from soils collected from widely distributed areas within their introduced range. The soil for this project was collected from three areas in western North America. With this design, we aim to determine species- and site-specific ability to create PSFs and if the type of PSF created is consistent in all soil from all three collection areas. The species examined are Agropyron cristatum, Centaurea solstitialis, Poa pratensis and Taeniatherum caput-medusae. Methods We used three-field collected soils (from northern Nevada, western Montana and eastern Montana) in a two-phase greenhouse experiment to quantify the type of PSFs created by four invasive species. The first phase was a conditioning phase wherein each invasive species created species- specific changes to the soil. The second phase of the experiment was the response phase wherein both the conditioning species and a native phytometer were grown in the conditioned soil and in unconditioned (control) soil. The final aboveground biomass was used to evaluate the effect of conditioning and to determine the type of PSF created by each invasive species. Important Findings Our results suggest that three of our four study species did show consistency in relation to PSF. Two species A. cristatum and T. caputmedusae consistently created PSF types that benefit conspecifics more than heterospecifics (and thus are 'invasive' PSF types) and P. pratensis consistently exhibited no, or 'neutral', feedbacks. The fourth species (C. solstitialis) was inconsistent: in one soil, no feedback was created; in other soil, an invasive PSF was created and in the last soil, a feedback that relatively benefited the native phytometer was created. Thus, PSFs appear to uniformly contribute to the success of two species (A. cristatum and T. caput-medusae) but not C. solstitialis nor P. pratensis. C1 [Perkins, Lora B.] S Dakota State Univ, Dept Nat Resource Management, 1 N Campus Dr, Brookings, SD 57007 USA. [Hatfield, Gary] S Dakota State Univ, Dept Math & Stat, 100 Admin Lane, Brookings, SD 57007 USA. [Espeland, Erin K.] USDA ARS, NPARL, Pest Management Res Unit, 1500 North Cent Ave, Sidney, MT 59720 USA. RP Perkins, LB (reprint author), S Dakota State Univ, Dept Nat Resource Management, 1 N Campus Dr, Brookings, SD 57007 USA. EM perkinslb@gmail.com FU South Dakota Agricultural Experiment Station; Nevada Agricultural Experiment Station FX South Dakota Agricultural Experiment Station; Nevada Agricultural Experiment Station. NR 47 TC 0 Z9 0 U1 14 U2 24 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1752-9921 EI 1752-993X J9 J PLANT ECOL JI J. Plant Ecol. PD APR PY 2016 VL 9 IS 2 BP 180 EP 186 DI 10.1093/jpe/rtv040 PG 7 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DJ4QN UT WOS:000374192300008 ER PT J AU Qin, JW Kim, MS Schmidt, WF Cho, BK Peng, YK Chao, KL AF Qin, Jianwei Kim, Moon S. Schmidt, Walter F. Cho, Byoung-Kwan Peng, Yankun Chao, Kuanglin TI A line-scan hyperspectral Raman system for spatially offset Raman spectroscopy SO JOURNAL OF RAMAN SPECTROSCOPY LA English DT Article DE SORS; subsurface detection; butter; melamine; food safety ID OPTICAL-PROPERTIES; PROBE AB Spatially offset Raman spectroscopy (SORS) is a technique that can obtain subsurface layered information by collecting Raman spectra from a series of surface positions laterally offset from the excitation laser. Currently optical fiber probes are used as major tools in SORS measurement, which are either slow (single fiber probe with mechanical movement) or restricted in selecting offset range and interval (fiber probe array). This study proposes a new method to conduct SORS measurement based on a newly developed line-scan hyperspectral Raman imaging system. A 785-nm point laser was used as an excitation source. A detection module consisting of an imaging spectrograph and a charge-coupled device camera was used to acquire line-shape SORS data in a spectral region of -592 to 3015cm(-1). Using a single scan, the system allowed simultaneous collection of a series of Raman spectra in a broad offset range (e.g. 0-36mm in two sides of the incident laser) with a narrow interval (e.g. 0.07mm). Four layered samples were created by placing butter slices with thicknesses of 1, 4, 7, and 10mm on top of melamine powder, providing different individual Raman characteristics to test the line-scan SORS technique. Self-modeling mixture analysis (SMA) was used to analyze the SORS data. Raman spectra from butter and melamine were successfully retrieved for all four butter-on-melamine samples using the SMA method. The line-scan SORS measurement technique provides a flexible and efficient method for subsurface evaluation, which has potential to be used for food safety and quality inspection. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Qin, Jianwei; Kim, Moon S.; Schmidt, Walter F.; Chao, Kuanglin] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Biosyst Machinery Engn, Coll Agr & Life Sci, 99 Daehak Ro, Taejon 305764, South Korea. [Peng, Yankun] China Agr Univ, Coll Engn, 17 Qinghua East Rd, Beijing 100083, Peoples R China. RP Chao, KL (reprint author), ARS, USDA, EMFSL, BARC East, Bldg 303,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM kevin.chao@ars.usda.gov FU Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea FX This research was partially supported by Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea. NR 22 TC 6 Z9 7 U1 9 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0377-0486 EI 1097-4555 J9 J RAMAN SPECTROSC JI J. Raman Spectrosc. PD APR PY 2016 VL 47 IS 4 BP 437 EP 443 DI 10.1002/jrs.4825 PG 7 WC Spectroscopy SC Spectroscopy GA DJ3XK UT WOS:000374139800010 ER PT J AU McCallum, S Graham, J Jorgensen, L Rowland, LJ Bassil, NV Hancock, JF Wheeler, EJ Vining, K Poland, JA Olmstead, JW Buck, E Wiedow, C Jackson, E Brown, A Hackett, CA AF McCallum, Susan Graham, Julie Jorgensen, Linzi Rowland, Lisa J. Bassil, Nahla V. Hancock, James F. Wheeler, Edmund J. Vining, Kelly Poland, Jesse A. Olmstead, James W. Buck, Emily Wiedow, Claudia Jackson, Eric Brown, Allan Hackett, Christine A. TI Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing SO MOLECULAR BREEDING LA English DT Article DE Vaccinium corymbosum; Autotetraploid; TetraploidMap; Genotyping by sequencing ID QUANTITATIVE TRAIT LOCI; TETRAPLOID POTATO; MOLECULAR MARKERS; GENE SEGREGATION; COLD TOLERANCE; VACCINIUM; QTL; RESISTANCE; MICROSATELLITE; IDENTIFICATION AB The construction of the first genetic map in autotetraploid blueberry has been made possible by the development of new SNP markers developed using genotyping by sequencing in a mapping population created from a cross between two key highbush blueberry cultivars, Draper x Jewel (Vaccinium corymbosum). The novel SNP markers were supplemented with existing SSR markers to enable the alignment of parental maps. In total, 1794 single nucleotide polymorphic (SNP) markers and 233 simple sequence repeat (SSR) markers exhibited segregation patterns consistent with a random chromosomal segregation model for meiosis in an autotetraploid. Of these, 700 SNPs and 85 SSRs were utilized for construction of the 'Draper' genetic map, and 450 SNPs and 86 SSRs for the 'Jewel' map. The 'Draper' map comprises 12 linkage groups (LG), associated with the haploid chromosome number for blueberry, and totals 1621 cM while the 'Jewel' map comprises 20 linkage groups totalling 1610 cM. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents. C1 [McCallum, Susan; Graham, Julie; Jorgensen, Linzi] James Hutton Inst, Cell & Mol Sci Programme, Dundee, Scotland. [Rowland, Lisa J.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD USA. [Bassil, Nahla V.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR USA. [Hancock, James F.] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Wheeler, Edmund J.] Berry Blue, MBG Mkt, Grand Junction, MI USA. [Vining, Kelly] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Poland, Jesse A.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Manhattan, KS 66506 USA. [Poland, Jesse A.] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Olmstead, James W.] Univ Florida, Dept Hort Sci, Gainesville, FL USA. [Buck, Emily; Wiedow, Claudia] New Zealand Inst Plant & Food Res Ltd, Private Bag 11600, Palmerston North 4442, New Zealand. [Jackson, Eric] Wheat Innovat Ctr, Gen Mills Crop Biosci, Manhattan, KS 66502 USA. [Brown, Allan] N Carolina State Univ, Dept Hort Sci, Plants Human Hlth Inst, Kannapolis, NC USA. [Hackett, Christine A.] Biomath & Stat Scotland, Dundee, Scotland. RP McCallum, S (reprint author), James Hutton Inst, Cell & Mol Sci Programme, Dundee, Scotland. EM Susan.McCallum@hutton.ac.uk RI Wiedow, Claudia/O-9291-2016; Buck, Emily/P-2687-2016; OI Wiedow, Claudia/0000-0003-3402-5460; Buck, Emily/0000-0002-3292-1694; Poland, Jesse/0000-0002-7856-1399 FU Horticulture Link [HL0190]; Specialty Crop Research Initiative project [2008-51180-04861]; Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS) FX This work was funded through Horticulture Link (HL0190), and all contributing partners are gratefully acknowledged. Project collaboration with the Specialty Crop Research Initiative-funded project (Grant 2008-51180-04861 entitled 'Generating genomic tools for blueberry improvement') has provided valuable access to plant material, genetic resources and advice. Support for this work from the Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS) is gratefully acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the James Hutton Institute or any of the other agencies involved in this research. We thank all reviewers for their comments and Sue Gardiner for assisting in preparation of this ms. NR 53 TC 1 Z9 1 U1 11 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD APR PY 2016 VL 36 IS 4 AR 41 DI 10.1007/s11032-016-0443-5 PG 24 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8IU UT WOS:000374456800006 ER PT J AU Perez-Vich, B del Moral, L Velasco, L Bushman, BS Knapp, SJ Leon, A Fernandez-Martinez, JM Berry, ST AF Perez-Vich, B. del Moral, L. Velasco, L. Bushman, B. S. Knapp, S. J. Leon, A. Fernandez-Martinez, J. M. Berry, S. T. TI Molecular basis of the high-palmitic acid trait in sunflower seed oil SO MOLECULAR BREEDING LA English DT Article DE Sunflower; Helianthus annuus; Palmitic acid; Candidate genes; 3-Ketoacyl-ACP synthase II; KASII ID HELIANTHUS-ANNUUS L; FATTY-ACID; CULTIVATED SUNFLOWER; STEARIC-ACID; 3-OXOACYL-ACP REDUCTASE; MARKER ANALYSIS; INBRED LINES; LINKAGE MAP; MUTANT; LOCI AB Sunflower (Helianthus annuus L.) seed oil with high palmitic acid content has enhanced thermo-oxidative stability, which makes it well suited to high-temperature uses. CAS-5 is a sunflower mutant line that accumulates over 25 % palmitic acid in its seed oil, compared to 5-8 % in conventional cultivars. The objective of this study was to investigate the molecular basis of the high-palmitic acid trait in CAS-5 through both candidate gene and QTL mapping approaches. An F-2 population derived from the cross between CAS-5 and the conventional line HA-89 was developed. A 3-ketoacyl-ACP synthase II (KASII) locus on a telomeric region of linkage group (LG) 9 of the sunflower genetic map was found to co-segregate with palmitic acid content in this population. The KASII locus explained the vast majority of the phenotypic variation (98 %) of the trait. Two minor QTL affecting palmitic acid content were also found on the lower half of LG 9 and on LG 17. Additionally, QTL associated with other major fatty acids (stearic, oleic, and linoleic acid) were identified on LG 1, 6, and 10. This result may reflect untapped genetic variation that could exist among sunflower cultivars for genes determining fatty acid composition. In addition to demonstrating the major role of a KASII locus in the accumulation of high levels of palmitic acid in CAS-5 seeds, this study stressed the importance of characterizing genes with minor effects on fatty acid profile in order to establish optimal breeding strategies for modifying fatty acid composition in sunflower seed oil. C1 [Perez-Vich, B.; del Moral, L.; Velasco, L.; Fernandez-Martinez, J. M.] Inst Agr Sostenible IAS CSIC, Alameda Obispo S-N, Cordoba 14004, Spain. [Bushman, B. S.] ARS, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. [Knapp, S. J.] Univ Calif Davis, Plant Breeding Ctr, Dept Plant Sci, One Shields Av, Davis, CA 95616 USA. [Leon, A.] Advanta Seeds JLT, Suite 206,Swiss Tower,Jumeirah Lake Towers, Dubai, U Arab Emirates. [Berry, S. T.] Limagrain UK Ltd, Stn Rd, Docking PE31 8LS, Norfolk, England. RP Perez-Vich, B (reprint author), Inst Agr Sostenible IAS CSIC, Alameda Obispo S-N, Cordoba 14004, Spain. EM bperez@ias.csic.es RI Velasco, Leonardo/C-5930-2008; OI Velasco, Leonardo/0000-0003-4998-9406; Fernandez Martinez, Jose Maria/0000-0003-1413-0642 FU Junta de Andalucia [AGR-118] FX The research was partially funded by Junta de Andalucia support program to research group AGR-118. NR 60 TC 0 Z9 0 U1 4 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD APR PY 2016 VL 36 IS 4 AR 43 DI 10.1007/s11032-016-0462-2 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8IU UT WOS:000374456800008 ER PT J AU Xiao, XY Ohm, HW Hunt, GJ Poland, JA Kong, LR Nemacheck, JA Williams, CE AF Xiao, Xiangye Ohm, Herbert W. Hunt, Greg J. Poland, Jesse A. Kong, Lingrang Nemacheck, Jill A. Williams, Christie E. TI Genotyping-by-sequencing to remap QTL for type II Fusarium head blight and leaf rust resistance in a wheat-tall wheatgrass introgression recombinant inbred population SO MOLECULAR BREEDING LA English DT Article DE Fusarium head blight resistance; Leaf rust resistance; Wheat; Tall wheatgrass; Substitution lines; KASP assay ID QUANTITATIVE TRAIT LOCI; PUCCINIA-TRITICINA; AGROPYRON-ELONGATUM; COMMON WHEAT; VIRULENCE; MARKERS; BARLEY; GRAIN; GENE; SCAB AB Fusarium graminearum Schwabe (Fusarium head blight, FHB) and Puccinia triticina Eriks (leaf rust) are two major fungal pathogens posing a continuous threat to the wheat crop; consequently, identifying resistance genes from various sources is always of importance to wheat breeders. We identified tightly linked single nucleotide polymorphism (SNP) markers for the FHB resistance quantitative trait locus (QTL) Qfhs. pur-7EL and the leaf rust resistance locus Lr19 using genotyping-by-sequencing (GBS) in a wheat-tall wheatgrass introgression-derived recombinant inbred line (RIL) population. One thousand and seven hundred high-confidence SNPs were used to conduct the linkage and QTL analysis. Qfhs. pur-7EL was mapped to a 2.9 cM region containing four markers within a 43.6 cM segment of wheatgrass chromosome 7el(2) that was translocated onto wheat chromosome 7DL. Lr19 from 7el(1) was mapped to a 1.21 cM region containing two markers in the same area, in repulsion. Five lines were identified with the resistance-associated SNP alleles for Qfhs. pur-7EL and Lr19 in coupling. Two SNP markers in the Qfhs. pur-7EL region were converted into PCR-based KASP markers. Investigation of the genetic characteristics of the parental lines of this RIL population indicated that they are translocation lines in two different wheat cultivar genetic backgrounds instead of 7E-7D substitution lines in Thatcher wheat background, as previously reported in the literature. C1 [Xiao, Xiangye; Ohm, Herbert W.; Williams, Christie E.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Hunt, Greg J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Poland, Jesse A.] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Kong, Lingrang] Shandong Agr Univ, State Key Lab Crop Biol, 61 Daizong Ave, Tai An 271018, Shandong, Peoples R China. [Nemacheck, Jill A.; Williams, Christie E.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Williams, CE (reprint author), Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. EM christie.williams@ars.usda.gov OI Poland, Jesse/0000-0002-7856-1399 FU USDA National Institute of Food and Agriculture competitive [10-85117-20607, 58-3620-8-688]; USDA-ARS CRIS [3602-22000-018-00D-18]; Agronomy Department, Purdue University; China Scholarship Council Fellowship FX This project was funded by USDA National Institute of Food and Agriculture competitive Grant Number 10-85117-20607 Award Number 58-3620-8-688 and USDA-ARS CRIS Number 3602-22000-018-00D-18. Mention of a commercial or proprietary product does not constitute endorsement or recommendation for its use by the USDA. The project was also funded by the Agronomy Department, Purdue University and the China Scholarship Council Fellowship. The authors wish to thank Shuangye Wu (Department of Pathology, Kansas State University) for assistance during GBS library construction. We appreciate Dr. Jessica Rutkoski (Plant Breeding and Genetics Department, Cornell University) who kindly provided advice for GBS analysis. We also thank Williams lab staff who assisted with DNA isolation. NR 48 TC 0 Z9 0 U1 11 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD APR PY 2016 VL 36 IS 4 AR 51 DI 10.1007/s11032-016-0472-0 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8IU UT WOS:000374456800016 ER PT J AU Weimer, PJ Kohn, RA AF Weimer, Paul J. Kohn, Richard A. TI Impacts of ruminal microorganisms on the production of fuels: how can we intercede from the outside? SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Review DE Biofuels; Carboxylate platform; Reactor microbiomes; Reverse beta-oxidation; Rumen ID VOLATILE FATTY-ACIDS; CARBOXYLATE PLATFORM; CLOSTRIDIUM KLUYVERI; REACTOR MICROBIOMES; CHAIN ELONGATION; BOVINE RUMEN; CAPROIC ACID; N-CAPROATE; ETHANOL; FERMENTATIONS AB The ruminal microbiome rapidly converts plant biomass to short-chain fatty acids (SCFA) that nourish the ruminant animal host. Because of its high species diversity, functional redundancy, and ease of extraruminal cultivation, this mixed microbial community is a particularly accomplished practitioner of the carboxylate platform for producing fuels and chemical precursors. Unlike reactor microbiomes derived from anaerobic digesters or sediments, the ruminal community naturally produces high concentrations of SCFA, with only modest methane production owing to the absence of both proton-reducing acetogens and aceticlastic methanogens. The extraruminal fermentation can be improved by addition of ethanol or lactate product streams, particularly in concert with reverse beta-oxidizing bacteria (e.g., Clostridium kluyveri or Megasphaera elsdenii) that facilitate production of valeric and caproic acids. Application of fundamental principles of thermodynamics allows identification of optimal conditions for SCFA chain elongation, as well as discovery of novel synthetic capabilities (e.g., medium-chain alcohol and alkane production) by this mixed culture system. C1 [Weimer, Paul J.] ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. [Weimer, Paul J.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Kohn, Richard A.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. RP Weimer, PJ (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA.; Weimer, PJ (reprint author), Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. EM Paul.Weimer@ars.usda.gov NR 50 TC 1 Z9 1 U1 13 U2 24 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD APR PY 2016 VL 100 IS 8 BP 3389 EP 3398 DI 10.1007/s00253-016-7358-2 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DI8IH UT WOS:000373744200001 PM 26879958 ER PT J AU Weimer, PJ Kohn, RA AF Weimer, Paul J. Kohn, Richard A. TI Impacts of ruminal microorganisms on the production of fuels: how can we intercede from the outside? (vol 100, pg 3389, 2016) SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Correction C1 [Weimer, Paul J.] ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden DriveWest, Madison, WI 53706 USA. [Weimer, Paul J.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Kohn, Richard A.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. RP Weimer, PJ (reprint author), ARS, USDA, US Dairy Forage Res Ctr, 1925 Linden DriveWest, Madison, WI 53706 USA.; Weimer, PJ (reprint author), Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. EM Paul.Weimer@ars.usda.gov NR 1 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD APR PY 2016 VL 100 IS 8 BP 3399 EP 3399 PG 1 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DI8IH UT WOS:000373744200002 PM 26960317 ER PT J AU Bansal, S Harrington, CA St Clair, JB AF Bansal, Sheel Harrington, Constance A. St Clair, John Bradley TI Tolerance to multiple climate stressors: a case study of Douglas-fir drought and cold hardiness SO ECOLOGY AND EVOLUTION LA English DT Article DE Abiotic stress; climate change; common garden; genetic variation; Pacific Northwest; precipitation gradient; principal components analysis; Pseudotsuga menziesii; temperature gradient ID PSEUDOTSUGA-MENZIESII; CUTICULAR TRANSPIRATION; ARABIDOPSIS-THALIANA; ADAPTATION; TRAITS; OREGON; FOREST; TEMPERATURE; POPULATIONS; MORTALITY AB Drought and freeze events are two of the most common forms of climate extremes which result in tree damage or death, and the frequency and intensity of both stressors may increase with climate change. Few studies have examined natural covariation in stress tolerance traits to cope with multiple stressors among wild plant populations. We assessed the capacity of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii), an ecologically and economically important species in the northwestern USA, to tolerate both drought and cold stress on 35 populations grown in common gardens. We used principal components analysis to combine drought and cold hardiness trait data into generalized stress hardiness traits to model geographic variation in hardiness as a function of climate across the Douglas-fir range. Drought and cold hardiness converged among populations along winter temperature gradients and diverged along summer precipitation gradients. Populations originating in regions with cold winters had relatively high tolerance to both drought and cold stress, which is likely due to overlapping adaptations for coping with winter desiccation. Populations from regions with dry summers had increased drought hardiness but reduced cold hardiness, suggesting a trade-off in tolerance mechanisms. Our findings highlight the necessity to look beyond bivariate trait-climate relationships and instead consider multiple traits and climate variables to effectively model and manage for the impacts of climate change on widespread species. C1 [Bansal, Sheel; Harrington, Constance A.] US Forest Serv, USDA, Pacific Northwest Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. [St Clair, John Bradley] US Forest Serv, USDA, Pacific Northwest Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Bansal, S (reprint author), US Geol Survey, Northern Prairie Wildlife Res Ctr, 8711 37th St SE, Jamestown, ND USA. EM sheelbansal9@gmail.com OI Bansal, Sheel/0000-0003-1233-1707 FU BLM FX We thank the BLM for funding support. NR 61 TC 0 Z9 0 U1 19 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD APR PY 2016 VL 6 IS 7 BP 2074 EP 2083 DI 10.1002/ece3.2007 PG 10 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DJ2RE UT WOS:000374052000016 PM 27099710 ER PT J AU McHugh, T AF McHugh, Tara TI How Dark Chocolate Is Processed SO FOOD TECHNOLOGY LA English DT Editorial Material C1 [McHugh, Tara] USDA ARS, Albany, CA USA. RP McHugh, T (reprint author), USDA ARS, Albany, CA USA. EM tara_mchugh@ars.usda.gov NR 0 TC 0 Z9 0 U1 5 U2 5 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD APR PY 2016 VL 70 IS 4 BP 134 EP 136 PG 3 WC Food Science & Technology SC Food Science & Technology GA DJ3DN UT WOS:000374085100017 ER PT J AU Kim, MS Hohenlohe, PA Kim, KH Seo, ST Klopfenstein, NB AF Kim, M. -S. Hohenlohe, P. A. Kim, K. -H. Seo, S. -T. Klopfenstein, N. B. TI Genetic diversity and population structure of Raffaelea quercus-mongolicae, a fungus associated with oak mortality in South Korea SO FOREST PATHOLOGY LA English DT Article ID GENOME AB Raffaelea quercus-mongolicae is a fungus associated with oak wilt and deemed to cause extensive oak mortality in South Korea. Since the discovery of this fungus on a dead Mongolian oak (Quercus mongolica) in 2004, the mortality continued to spread southwards in South Korea. Despite continued expansion of the disease and associated significant impacts on forest ecosystems, information is lacking about the origin and genetic diversity of R.quercus-mongolicae. Restriction-site-associated DNA (RAD) sequencing was used to assess genetic diversity and population structure among five populations (provinces) of R.quercus-mongolicae in South Korea. In total, 179 single nucleotide polymorphisms (SNPs) were identified among 2,639 RAD loci across the nuclear genome of the 54 R.quercus-mongolicae isolates (0.0012 SNPs per bp), which displayed an overall low expected heterozygosity and no apparent population structure. The low genetic diversity and no apparent population structure among South Korean populations of this ambrosia beetle-vectored fungus support the hypothesis that this fungus was introduced to South Korea. C1 [Kim, M. -S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul 02707, South Korea. [Hohenlohe, P. A.] Univ Idaho, Dept Biol Sci, Moscow, ID 83843 USA. [Hohenlohe, P. A.] Univ Idaho, Inst Bioinformat & Evolutionary Studies, Moscow, ID 83843 USA. [Kim, K. -H.; Seo, S. -T.] Korea Forest Res Inst, Div Forest Insect Pests & Dis, Seoul, South Korea. [Klopfenstein, N. B.] USDA Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. RP Kim, MS (reprint author), Kookmin Univ, Dept Forestry Environm & Syst, Seoul 02707, South Korea. EM mkim@kookmin.ac.kr FU Korea Forest Research Institute; NIH [P30 GM103324] FX This work was supported by the Korea Forest Research Institute. The authors thank Mr. John Hanna for his technical assistance. Use of trade names does not constitute endorsement by the USDA Forest Service. PAH received support from NIH grant P30 GM103324. NR 12 TC 0 Z9 0 U1 2 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 EI 1439-0329 J9 FOREST PATHOL JI Forest Pathol. PD APR PY 2016 VL 46 IS 2 BP 164 EP 167 DI 10.1111/efp.12263 PG 4 WC Forestry SC Forestry GA DJ2EF UT WOS:000374015800006 PM 27087782 ER PT J AU Pauli, D Andrade-Sanchez, P Carmo-Silva, AE Gazave, E French, AN Heun, J Hunsaker, DJ Lipka, AE Setter, TL Strand, RJ Thorp, KR Wang, S White, JW Gore, MA AF Pauli, Duke Andrade-Sanchez, Pedro Carmo-Silva, A. Elizabete Gazave, Elodie French, Andrew N. Heun, John Hunsaker, Douglas J. Lipka, Alexander E. Setter, Tim L. Strand, Robert J. Thorp, Kelly R. Wang, Sam White, Jeffrey W. Gore, Michael A. TI Field-Based High-Throughput Plant Phenotyping Reveals the Temporal Patterns of Quantitative Trait Loci Associated with Stress-Responsive Traits in Cotton SO G3-GENES GENOMES GENETICS LA English DT Article DE field-based HTPP; stress response; canopy temperature; NDVI; QTL ID CARBON-ISOTOPE DISCRIMINATION; GOSSYPIUM-HIRSUTUM L.; ABSCISIC-ACID; STOMATAL CONDUCTANCE; SPECTRAL REFLECTANCE; GENETIC PATTERNS; UPLAND COTTON; LINT YIELD; CROP; PHOTOSYNTHESIS AB The application of high-throughput plant phenotyping (HTPP) to continuously study plant populations under relevant growing conditions creates the possibility to more efficiently dissect the genetic basis of dynamic adaptive traits. Toward this end, we employed a field-based HTPP system that deployed sets of sensors to simultaneously measure canopy temperature, reflectance, and height on a cotton (Gossypium hirsutum L.) recombinant inbred line mapping population. The evaluation trials were conducted under well-watered and water-limited conditions in a replicated field experiment at a hot, arid location in central Arizona, with trait measurements taken at different times on multiple days across 2010-2012. Canopy temperature, normalized difference vegetation index (NDVI), height, and leaf area index (LAI) displayed moderate-to-high broad-sense heritabilities, as well as varied interactions among genotypes with water regime and time of day. Distinct temporal patterns of quantitative trait loci (QTL) expression were mostly observed for canopy temperature and NDVI, and varied across plant developmental stages. In addition, the strength of correlation between HTPP canopy traits and agronomic traits, such as lint yield, displayed a time-dependent relationship. We also found that the genomic position of some QTL controlling HTPP canopy traits were shared with those of QTL identified for agronomic and physiological traits. This work demonstrates the novel use of a field-based HTPP system to study the genetic basis of stress-adaptive traits in cotton, and these results have the potential to facilitate the development of stress-resilient cotton cultivars. C1 [Pauli, Duke; Gazave, Elodie; Gore, Michael A.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, 310 Bradfield Hall, Ithaca, NY 14853 USA. [Pauli, Duke; Gazave, Elodie; Gore, Michael A.] Cornell Univ, Sch Integrat Plant Sci, Soil & Crop Sci Sect, Ithaca, NY 14853 USA. [Andrade-Sanchez, Pedro; Heun, John] Univ Arizona, Maricopa Agr Ctr, Dept Agr & Biosyst Engn, Tucson, AZ 85138 USA. [Wang, Sam] Univ Arizona, Maricopa Agr Ctr, Sch Plant Sci, Tucson, AZ 85138 USA. [Carmo-Silva, A. Elizabete; French, Andrew N.; Hunsaker, Douglas J.; Strand, Robert J.; Thorp, Kelly R.; White, Jeffrey W.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Lipka, Alexander E.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Carmo-Silva, A. Elizabete] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Strand, Robert J.] LemnaTec Corp, St Louis, MO 63110 USA. [Wang, Sam] Bridgestone Amer Inc, Eloy, AZ 85131 USA. RP Gore, MA (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, 310 Bradfield Hall, Ithaca, NY 14853 USA. EM mag87@cornell.edu FU Cotton Incorporated Fellowship; Core Project Funds; Cornell University; United States Department of Agriculture-Agricultural Research Service (USDA-ARS); National Science Foundation [IOS1238187] FX We especially thank Kristen Cox, Bill Luckett, Joel Gilley, Virginia Moreno, Sara Wyckoff, Spencer Fosnot, and Brian Nadon for providing excellent technical expertise. This research was supported by Cotton Incorporated Fellowship (D.P.) and Core Project Funds (P.A.-S. and M.A.G.), Cornell University startup funds (M.A.G.), United States Department of Agriculture-Agricultural Research Service (USDA-ARS) (K.R.T., D.H., A.N.F., and J.W.W.), and National Science Foundation IOS1238187. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information, and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 67 TC 5 Z9 5 U1 14 U2 24 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD APR 1 PY 2016 VL 6 IS 4 BP 865 EP 879 DI 10.1534/g3.115.023515 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA DJ2VI UT WOS:000374062800010 PM 26818078 ER PT J AU Ramstein, GP Evans, J Kaeppler, SM Mitchell, RB Vogel, KP Buell, CR Casler, MD AF Ramstein, Guillaume P. Evans, Joseph Kaeppler, Shawn M. Mitchell, Robert B. Vogel, Kenneth P. Buell, C. Robin Casler, Michael D. TI Accuracy of Genomic Prediction in Switchgrass (Panicum virgatum L.) Improved by Accounting for Linkage Disequilibrium SO G3-GENES GENOMES GENETICS LA English DT Article DE genomic selection; linkage disequilibrium; exome capture; bioenergy; Panicum virgatum L; GenPred; Shared data resource ID ESTIMATED BREEDING VALUE; DAIRY-CATTLE BREEDS; NUCLEOTIDE POLYMORPHISM; RELATIONSHIP MATRICES; POPULATION-STRUCTURE; QUANTITATIVE TRAITS; VARIABLE SELECTION; MOLECULAR MARKERS; RIDGE-REGRESSION; FAMILY SELECTION AB Switchgrass is a relatively high-yielding and environmentally sustainable biomass crop, but further genetic gains in biomass yield must be achieved to make it an economically viable bioenergy feedstock. Genomic selection (GS) is an attractive technology to generate rapid genetic gains in switchgrass, and meet the goals of a substantial displacement of petroleum use with biofuels in the near future. In this study, we empirically assessed prediction procedures for genomic selection in two different populations, consisting of 137 and 110 half-sib families of switchgrass, tested in two locations in the United States for three agronomic traits: dry matter yield, plant height, and heading date. Marker data were produced for the families' parents by exome capture sequencing, generating up to 141,030 polymorphic markers with available genomic-location and annotation information. We evaluated prediction procedures that varied not only by learning schemes and prediction models, but also by the way the data were preprocessed to account for redundancy in marker information. More complex genomic prediction procedures were generally not significantly more accurate than the simplest procedure, likely due to limited population sizes. Nevertheless, a highly significant gain in prediction accuracy was achieved by transforming the marker data through a marker correlation matrix. Our results suggest that marker-data transformations and, more generally, the account of linkage disequilibrium among markers, offer valuable opportunities for improving prediction procedures in GS. Some of the achieved prediction accuracies should motivate implementation of GS in switchgrass breeding programs. C1 [Ramstein, Guillaume P.; Kaeppler, Shawn M.; Casler, Michael D.] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Kaeppler, Shawn M.] Univ Wisconsin, Dept Energy, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. [Evans, Joseph; Buell, C. Robin] Michigan State Univ, Dept Energy, Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA. [Evans, Joseph; Buell, C. Robin] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Mitchell, Robert B.; Vogel, Kenneth P.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, ARS, USDA, Lincoln, NE 68583 USA. [Casler, Michael D.] ARS, USDA, Madison, WI 53706 USA. RP Ramstein, GP (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM ramstein@wisc.edu FU US Department of Energy Great Lakes Bioenergy Research Center, Department of the Environment (DOE) Office of Science [BER DE-FC02- 07ER64494]; US Department of Energy Joint Genome Institute; Office of Science of the US Department of Energy [DE-AC02-05CH11231]; Agriculture and Food Research Initiative Competitive Grant from the Unites States Department of Agriculture (USDA) National Institute of Food and Agriculture (CenUSA) [2011-68005-30411]; USDA-ARS; University of Wisconsin Agricultural Research Stations; Gabelman-Shippo Wisconsin Distinguished Graduate Fellowship at the University of Wisconsin-Madison FX The authors thank two anonymous reviewers for remarks and suggestions that greatly helped with improving the manuscript. We are grateful to Jeremy Schmutz of the Department of Energy Joint Genome Institute and Hudson Alpha for his work on the switchgrass genome, and to Nick Baker and Joseph Halinar, USDA-ARS, Madison, WI, and Steve Masterson, USDA-ARS, Lincoln, NE, for assistance with field operations and data collection. This research was funded in part by the following agencies and organizations: the US Department of Energy Great Lakes Bioenergy Research Center, Department of the Environment (DOE) Office of Science BER DE-FC02- 07ER64494 (laboratory operations, genotyping, and bioinformatics), the US Department of Energy Joint Genome Institute, supported by the Office of Science of the US Department of Energy under Contract No. DE-AC02-05CH11231 (sequencing), Agriculture and Food Research Initiative Competitive Grant No. 2011-68005-30411 from the Unites States Department of Agriculture (USDA) National Institute of Food and Agriculture (CenUSA; field operations and phenotypic measurements), USDA-ARS Congressionally allocated funds (field operations, technical support, and logistics), and the University of Wisconsin Agricultural Research Stations (field operations). Mention of commercial products and organizations in this manuscript is solely to provide specific information. The USDA is an equal opportunity provider and employer. G.P.R. was supported by the Gabelman-Shippo Wisconsin Distinguished Graduate Fellowship at the University of Wisconsin-Madison. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 0 Z9 0 U1 1 U2 8 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD APR 1 PY 2016 VL 6 IS 4 BP 1049 EP 1062 DI 10.1534/g3.115.024950 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA DJ2VI UT WOS:000374062800024 PM 26869619 ER PT J AU Ligon, FK Nakamoto, RJ Harvey, BC Baker, PF AF Ligon, F. K. Nakamoto, R. J. Harvey, B. C. Baker, P. F. TI Use of streambed substrate as refuge by steelhead or rainbow trout Oncorhynchus mykiss during simulated freshets SO JOURNAL OF FISH BIOLOGY LA English DT Article DE displacement; embeddedness; flood; high flows; interstitial; velocity ID JUVENILE COHO SALMON; WINTER CONCEALMENT HABITAT; SPRING CHINOOK SALMON; RONDE RIVER-BASIN; ATLANTIC SALMON; BROWN TROUT; AGGRESSIVE-BEHAVIOR; POPULATION-DENSITY; OREGON; FLOW AB A flume was used to estimate the carrying capacity of streambed substrates for juvenile steelhead or rainbow trout Oncorhynchus mykiss seeking refuge from simulated freshets. The simulated freshets had mean water column velocities of c. 11ms(-1). The number of O. mykiss finding cover within the interstices of the substrate was documented for different substrate sizes and levels of embeddedness. The availability of suitable refuges determined the carrying capacity of the substrate for O. mykiss. For the size of the O. mykiss tested [mean +/- s.d. fork length (L-F)=122 +/- 126mm], the number of interstices with depths 200mm measured with a 140mm diameter flexible plastic tube was the best predictor of the number of O. mykiss able to find cover (r(2)=075). Oncorhynchus mykiss seeking refuge from freshets may need deeper interstices than those seeking concealment at autumn or winter base flows. The availability of interstices suitable as refuge from high flows may determine autumn and winter carrying capacity. (C) 2016 The Fisheries Society of the British Isles C1 [Ligon, F. K.] Stillwater Sci, 850 G St,Ste K, Arcata, CA 95521 USA. [Nakamoto, R. J.; Harvey, B. C.] USDA, Forest Serv, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. [Baker, P. F.] Stillwater Sci, 2855 Telegraph Ave,Ste 400, Berkeley, CA 94705 USA. RP Ligon, FK (reprint author), Stillwater Sci, 850 G St,Ste K, Arcata, CA 95521 USA. EM frank@stillwatersci.com FU Stillwater Sciences FX Stillwater Sciences provided partial funding. Discussions with W. Dietrich concerning channel morphology and streambed composition helped greatly. D. Pederson, J. White, N. Lassettre and M. Cover provided assistance during the experiment. Comments by C. Champe, A. Percival and W. Dietrich improved the manuscript. K. Rodriguez and S. Khandwala helped with figures. NR 52 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1112 EI 1095-8649 J9 J FISH BIOL JI J. Fish Biol. PD APR PY 2016 VL 88 IS 4 BP 1475 EP 1485 DI 10.1111/jfb.12925 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DJ2BO UT WOS:000374008900012 PM 26932205 ER PT J AU Sedej, I Milczarek, R Wang, SC Sheng, RQ Avena-Bustillos, RD Dao, L Takeoka, G AF Sedej, Ivana Milczarek, Rebecca Wang, Selina C. Sheng, Runqi Avena-Bustillos, Roberto de Jesus Dao, Lan Takeoka, Gary TI Membrane-Filtered Olive Mill Wastewater: Quality Assessment of the Dried Phenolic-Rich Fraction SO JOURNAL OF FOOD SCIENCE LA English DT Article DE drying; membrane filtration; olive mill wastewater; phenolics ID ANTIOXIDANT PROPERTIES; HOT AIR; EXTRACTS; SYSTEM; OIL; ULTRAFILTRATION; WASTEWATERS; SEPARATION; POWDER AB A current trend in olive mill wastewater (OMWW) management is to not only decrease environmental pollution but also to extract and utilize valuable by-products. Therefore, the objectives of this study were to explore different techniques for drying a phenolic-rich membrane filtration fraction of OMWW and compare the techniques in terms of the dried product quality and feasibility of the process. The OMWW from 2 (3-phase and 2-phase) California mills was subjected to a 2-step membrane filtration process using a novel vibratory system. The reverse osmosis retentate (RO-R) is a phenolic-rich coproduct stream, and the reverse osmosis permeate is a near-pure water stream that could be recycled into the milling process. Spray-, freeze-, and infrared-drying were applied to obtain solid material from the RO-R. Drying of the RO-R was made possible only with addition of 10% maltodextrin as a carrier. The total soluble phenolics in dried RO-R were in the range 0.15 to 0.58 mg gallic acid equivalents/g of dry weight for 2-phase RO-R, and 1.38 to 2.17 mg gallic acid equivalents/g of dry weight for the 3-phase RO-R. Spray-dried RO-R from 3-phase OMWW showed remarkable antioxidant activity. Protocatechuic acid, tyrosol, vanillic acid, and p-coumaric acid were quantified in all dried RO-R, whereas 3-hydroxytyrosol was found in 3-phase dried RO-R. This combination of separation and drying technologies helps to add value and shelf-stability to an olive oil by-product and increase environmental sustainability of its production. C1 [Sedej, Ivana; Wang, Selina C.; Sheng, Runqi] Univ Calif Davis, Olive Ctr, 392 Old Davis Rd, Davis, CA 95616 USA. [Milczarek, Rebecca; Avena-Bustillos, Roberto de Jesus; Dao, Lan; Takeoka, Gary] USDA ARS, Hlth Proc Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. RP Sedej, I (reprint author), Univ Calif Davis, Olive Ctr, 392 Old Davis Rd, Davis, CA 95616 USA. EM ivana.sedej@ars.usda.gov FU California Dept. of Food and Agriculture [SCB12040] FX This work is a part of the Specialty Crop Block Grant Program - SCB12040, supported by the California Dept. of Food and Agriculture. OMWW samples were generously donated by California Olive Ranch (Artois, Calif., U.S.A.) and Seka Hills (Brooks, Calif., U.S.A.). S.C.W. and R.S. are grateful to New Logic Research, Inc. (Emeryville, Calif., U.S.A.) for providing the filtration system and training of its operations. NR 39 TC 2 Z9 2 U1 5 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD APR PY 2016 VL 81 IS 4 BP E889 EP E896 DI 10.1111/1750-3841.13267 PG 8 WC Food Science & Technology SC Food Science & Technology GA DJ2PW UT WOS:000374048200009 PM 26989993 ER PT J AU Abd-Alla, AMM Kariithi, HM Cousserans, FO Parker, NJ Ince, IA Scully, ED Boeren, S Geib, SM Mekonnen, S Vlak, JM Parker, AG Vreysen, MJB Bergoin, M AF Abd-Alla, Adly M. M. Kariithi, Henry M. Cousserans, Franc Ois Parker, Nicolas J. Ince, Ikbal Agah Scully, Erin D. Boeren, Sjef Geib, Scott M. Mekonnen, Solomon Vlak, Just M. Parker, Andrew G. Vreysen, Marc J. B. Bergoin, Max TI Comprehensive annotation of Glossina pallidipes salivary gland hypertrophy virus from Ethiopian tsetse flies: a proteogenomics approach SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID PROTEIN FAMILIES DATABASE; NON-AUG CODONS; NUCLEOPOLYHEDROVIRUS POPULATION; LABORATORY COLONIES; DNA-SEQUENCES; GENOME; EVOLUTION; FLY; IDENTIFICATION; GENOTYPES AB Glossina pallidipes salivary gland hypertrophy virus (GpSGHV; family Hytrosaviridae) can establish asymptomatic and symptomatic infection in its tsetse fly host. Here, we present a comprehensive annotation of the genome of an Ethiopian GpSGHV isolate (GpSGHV-Eth) compared with the reference Ugandan GpSGHV isolate (GpSGHV-Uga; GenBank accession number EF568108). GpSGHV-Eth has higher salivary gland hypertrophy syndrome prevalence than GpSGHV-Uga. We show that the GpSGHV-Eth genome has 190 291 nt, a low G+C content (27.9 %) and encodes 174 putative ORFs. Using proteogenomic and transcriptome mapping, 141 and 86 ORFs were mapped by transcripts and peptides, respectively. Furthermore, of the 174 ORFs, 132 had putative transcriptional signals [TATA-like box and poly(A) signals]. Sixty ORFs had both TATA-like box promoter and poly(A) signals, and mapped by both transcripts and peptides, implying that these ORFs encode functional proteins. Of the 60 ORFs, 10 ORFs are homologues to baculovirus and nudivirus core genes, including three per os infectivity factors and four RNA polymerase subunits (LEF4, 5, 8 and 9). Whereas GpSGHV-Eth and GpSGHV-Uga are 98.1 % similar at the nucleotide level, 37 ORFs in the GpSGHV-Eth genome had nucleotide insertions (n=17) and deletions (n=20) compared with their homologues in GpSGHV-Uga. Furthermore, compared with the GpSGHV-Uga genome, 11 and 24 GpSGHV ORFs were deleted and novel, respectively. Further, 13 GpSGHV-Eth ORFs were non-canonical; they had either CTG or TTG start codons instead of ATG. Taken together, these data suggest that GpSGHV-Eth and GpSGHV-Uga represent two different lineages of the same virus. Genetic differences combined with host and environmental factors possibly explain the differential GpSGHV pathogenesis observed in different G. pallidipes colonies. C1 [Abd-Alla, Adly M. M.; Kariithi, Henry M.; Parker, Andrew G.; Vreysen, Marc J. B.] IAEA, Joint FAO IAEA Div Nucl Techn Food & Agr, Insect Pest Control Labs, A-1400 Vienna, Austria. [Kariithi, Henry M.] Kenya Agr & Livestock Res Org, Biotechnol Res Inst, POB 57811, Nairobi, Kenya. [Kariithi, Henry M.; Vlak, Just M.] Wageningen Univ, Virol Lab, NL-6708 PB Wageningen, Netherlands. [Cousserans, Franc Ois; Bergoin, Max] Univ Montpellier, Fac Sci, Lab Pathol Comparee, F-34095 Montpellier, France. [Parker, Nicolas J.] 10 Lockhart Close, Kenilworth CV8 1RB, Warwick, England. [Ince, Ikbal Agah] Acibadem Univ, Sch Med, Dept Med Microbiol, TR-34752 Istanbul, Turkey. [Scully, Erin D.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, USDA ARS, East Campus, Lincoln, NE 68583 USA. [Boeren, Sjef] Wageningen Univ, Biochem Lab, NL-6703 HA Wageningen, Netherlands. [Geib, Scott M.] ARS, Trop Crop & Commod Protect Res Unit, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Mekonnen, Solomon] NICETT, Addis Ababa, Ethiopia. RP Abd-Alla, AMM (reprint author), IAEA, Joint FAO IAEA Div Nucl Techn Food & Agr, Insect Pest Control Labs, A-1400 Vienna, Austria. EM a.m.m.abd-alla@iaea.org OI ABD-ALLA, Adly/0000-0001-7540-4462 FU Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, Austria FX This work was funded by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA, Austria. The authors acknowledge Ms Irene Meki, Mr Moges Hidoto, and the staff members of NICETT and IPCL for technical assistance. NR 76 TC 1 Z9 1 U1 0 U2 2 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD APR PY 2016 VL 97 BP 1010 EP 1031 DI 10.1099/jgv.0.000409 PN 4 PG 22 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA DJ3MF UT WOS:000374108900020 PM 26801744 ER PT J AU Peterson, BC Flora, C Wood, M Bosworth, BG Quiniou, SM Greenway, TE Byars, TS Wise, DJ AF Peterson, Brian C. Flora, Corrin Wood, Monica Bosworth, Brian G. Quiniou, Sylvie M. Greenway, Terrence E. Byars, Todd S. Wise, David J. TI Vaccination of Full-sib Channel Catfish Families Against Enteric Septicemia of Catfish with an Oral Live Attenuated Edwardsiella ictaluri Vaccine SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID RESISTANCE; ISOLATE AB This study evaluated the efficacy of an oral live attenuated Edwardsiella ictaluri vaccine against enteric septicemia of catfish in 20 full-sib fingerling channel catfish families. The vaccine was delivered orally by feeding fish a diet coated with an attenuated E. ictaluri isolate. Sixty-nine days postvaccination, control and vaccinated fish were challenged with virulent E. ictaluri and mortality was examined for 21d postchallenge. Vaccinated fish had significantly lower mortality than nonvaccinated fish following challenge (P<0.001). Mortality of vaccinated fish was 1.7 +/- 1.4% as opposed to 47.8 +/- 28.7% in nonvaccinated fish. Relative percent survival ranged from 87.7 to 100% with an average of 95.2 +/- 4.0% (+/- SE) among the 20 families of fish. There were significant differences in mortality among families in nonvaccinated fish (P<0.01) while there were no differences among vaccinated families of fish. Results indicate that the live attenuated E. ictaluri vaccine is effective at reducing mortality in channel catfish exposed to virulent E. ictaluri. These data demonstrate that genetic differences among healthy families of channel catfish are not major considerations in developing an effective vaccination program utilizing the oral vaccination platform described in this study. C1 [Peterson, Brian C.; Flora, Corrin; Wood, Monica; Bosworth, Brian G.; Quiniou, Sylvie M.] ARS, USDA, Thad Cochran Natl Warmwater Aquaculture Ctr, Warmwater Aquaculture Res Unit, 127 Expt Stn Rd,POB 38, Stoneville, MS 38776 USA. [Greenway, Terrence E.; Byars, Todd S.; Wise, David J.] Delta Res & Extens Ctr, Thad Cochran Natl Warmwater Aquaculture Ctr, Mississippi Agr & Forestry Expt Stn, 127 Expt Stn Rd,POB 197, Stoneville, MS 38776 USA. RP Peterson, BC (reprint author), ARS, USDA, Thad Cochran Natl Warmwater Aquaculture Ctr, Warmwater Aquaculture Res Unit, 127 Expt Stn Rd,POB 38, Stoneville, MS 38776 USA. EM brian.peterson@ars.usda.gov NR 16 TC 1 Z9 1 U1 1 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD APR PY 2016 VL 47 IS 2 BP 207 EP 211 DI 10.1111/jwas.12264 PG 5 WC Fisheries SC Fisheries GA DI9GF UT WOS:000373809500004 ER PT J AU O'Donnell, KM Thompson, FR Semlitsch, RD AF O'Donnell, K. M. Thompson, F. R. Semlitsch, R. D. TI Prescribed fire alters surface activity and movement behavior of a terrestrial salamander SO JOURNAL OF ZOOLOGY LA English DT Article DE amphibian; forest management; minimum convex polygon; radio-frequency identification; Plethodon albagula; plethodontid; prescribed fire; fire management ID BROOK EXPERIMENTAL FOREST; PLETHODON-CINEREUS; UNITED-STATES; NEW-HAMPSHIRE; MANAGEMENT; INVERTEBRATES; POPULATIONS; DISPERSAL; ABUNDANCE; MISSOURI AB Prescribed fire has become a commonly used forest management tool for reducing the occurrence of severe wildfires, decreasing fuel loads and reestablishing the historic ecological influences of fire. Investigating population-level wildlife responses to prescribed fire is important for evaluating the effects of fire management on animals, but in order to predict and simulate wildlife responses to potential management actions, mechanistic studies that elucidate individual-level responses are also necessary. We used radio-frequency identification to investigate individual-level responses of western slimy salamanders Plethodon albagula to prescribed fire. We compared the salamander home-range sizes [minimum convex polygons (MCP)], movement behaviors, and activity levels before and after prescribed fire using a randomized block experiment. We initiated the study at three plots in 2011, and added two new plots in 2012. We captured, tagged and released 38 salamanders at each plot from May 2011-May 2012 (total N=205). We recorded 918 total recaptures of 142 unique salamanders; 31% of tagged salamanders were never recaptured. Across all years and treatments, only 14% of relocations were surface-active (i.e. visually confirmed) salamanders; the others were underground. Following prescribed fire, the surface-active proportion of recaptures was nearly seven times greater in control versus burned areas, which indicates that P.albagula respond to post-prescribed fire conditions by spending more time belowground. We did not find evidence of direct mortality of salamanders from fires; the proportion of known-alive individuals did not differ between treatments. Maximum daily displacement was 43.3% higher among burn-area than control-area salamanders, and MCP varied in a similar pattern; this may indicate salamanders were attempting to find more hospitable microenvironments or other resources, such as prey. Together, these individual-level observations corroborate our findings from a population-level study of a congeneric terrestrial salamander and contribute to our understanding of the behavioral mechanisms underlying population dynamics. C1 [O'Donnell, K. M.; Semlitsch, R. D.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Thompson, F. R.] US Forest Serv, No Res Stn, USDA, Columbia, MO USA. RP O'Donnell, KM (reprint author), US Geol Survey, Wetland & Aquat Res Ctr, 7920 NW 71st St, Gainesville, FL 32653 USA. EM odonnell.katie.m@gmail.com FU U.S. Forest Service Northern Research Station through Cooperative [10-JV-11242311-061]; University of Missouri FX We thank A. Milo, A. Senters, D. Drake, N. Thompson, A. Hopping, P. Fisher, G. Connette, B. Peterman and M. Osbourn for field assistance; and A. George and S. Pittman for valuable discussions on movement ecology. J. Bakameyer led planning and execution of prescribed burns and provided GIS data. Reviews from S. Pittman, A. Messerman, D.S. Godwin, J. Homyack and an anonymous reviewer greatly improved earlier drafts of this manuscript. K.M.O. was supported by a GAANN fellowship. Sampling and procedures were approved by the Missouri Department of Conservation and the MU Animal Care and Use Committee Protocol 7403. Support for this study was provided by the U.S. Forest Service Northern Research Station through Cooperative Agreement 10-JV-11242311-061 and the University of Missouri. NR 42 TC 0 Z9 0 U1 22 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0952-8369 EI 1469-7998 J9 J ZOOL JI J. Zool. PD APR PY 2016 VL 298 IS 4 BP 303 EP 309 DI 10.1111/jzo.12316 PG 7 WC Zoology SC Zoology GA DJ4HY UT WOS:000374167200009 ER PT J AU Mushayabasa, S Posny, D Wang, J AF Mushayabasa, Steady Posny, Drew Wang, Jin TI MODELING THE INTRINSIC DYNAMICS OF FOOT-AND-MOUTH DISEASE SO MATHEMATICAL BIOSCIENCES AND ENGINEERING LA English DT Article DE Foot-and-mouth disease; mathematical models; threshold dynamics ID VIRUS; TRANSMISSION; VACCINATION; IDENTIFICATION; ENVIRONMENTS; STRATEGIES; EPIDEMIC; NUMBERS AB We propose a new mathematical modeling framework to investigate the transmission and spread of foot-and-mouth disease. Our models incorporate relevant biological and ecological factors, vaccination effects, and seasonal impacts during the complex interaction among susceptible, vaccinated, exposed, infected, carrier, and recovered animals. We conduct both epidemic and endemic analysis, with a focus on the threshold dynamics characterized by the basic reproduction numbers. In addition, numerical simulation results are presented to demonstrate the analytical findings. C1 [Mushayabasa, Steady] Univ Zimbabwe, Dept Math, POB MP 167, Harare, Zimbabwe. [Posny, Drew] NC State Univ, NSF Ctr Integrated Pest Management, Raleigh, NC 27606 USA. [Posny, Drew] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Wang, Jin] Univ Tennessee, Dept Math, Chattanooga, TN 37403 USA. RP Wang, J (reprint author), Univ Tennessee, Dept Math, Chattanooga, TN 37403 USA. EM steadymushaya@gmail.com; drew.posny@ars.usda.gov; Jin-Wang02@utc.edu FU National Science Foundation [1412826, 1557739] FX The work of JW is partially supported by the National Science Foundation under Grant Numbers 1412826 and 1557739. The authors are grateful to the two anonymous reviewers for their comments to improve this paper. NR 23 TC 0 Z9 0 U1 2 U2 3 PU AMER INST MATHEMATICAL SCIENCES-AIMS PI SPRINGFIELD PA PO BOX 2604, SPRINGFIELD, MO 65801-2604 USA SN 1547-1063 EI 1551-0018 J9 MATH BIOSCI ENG JI Math. Biosci. Eng. PD APR PY 2016 VL 13 IS 2 BP 425 EP 442 DI 10.3934/mbe.2015010 PG 18 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA DJ0YE UT WOS:000373929500010 PM 27105988 ER PT J AU Qin, YL Yu, LX Wu, RC Yang, DJ Qiu, XQ Zhu, JY AF Qin, Yanlin Yu, Lixuan Wu, Ruchun Yang, Dongjie Qiu, Xueqing Zhu, J. Y. TI Biorefinery Lignosulfonates from Sulfite-Pretreated Softwoods as Dispersant for Graphite SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Lignosulfonate; Graphite; Dispersion stability; Lignin-coproducts; Biorefinery ID FIR FOREST RESIDUE; COAL-WATER SLURRY; AQUEOUS DISPERSIONS; HIGH-TITER; GRAPHENE; LIGNIN; STABILITY; BIOCONVERSION; SPORL; OXIDE AB Two biorefinery lignosulfonates (LSs), Ca-LS-DF and Na-LS-LP, were, respectively, isolated from pilot-scale sulfite-pretreated spent liquor of lodgepole pine and fermentation residue of Douglas-fir harvest forest residue. The molecular weights of Na-LS-LP and Ca-LS-DF were approximately 9 000 and 11 000 Da, respectively. The two LSs were applied as dispersant for graphite in aqueous suspensions. The dispersion stability was evaluated by a scanning electron microscope and Turbiscan Lab Expert. LS performance in modifying graphite was better than that of a commercial dispersant Reax-85A as indicated by the Turbiscan TSI values, zeta potential of suspension particles, and SEM imaging. The practical importance of this study lies in the fact that the pilot scale sulfite pretreatments that produced the two LSs also produced excellent bioethanol yields at high titer without detoxification and washing, suggesting the LSs are a true value-added coproduct forhigh yield biofuel production. C1 [Qin, Yanlin; Yu, Lixuan; Yang, Dongjie; Qiu, Xueqing] S China Univ Technol, Sch Chem & Chem Engn, 381 Wushan Rd, Guangzhou 510641, Guangdong, Peoples R China. [Wu, Ruchun] Guangxi Univ Nationalities, Sch Chem & Chem Engn, 188 Daxue East Rd, Nanning, Peoples R China. [Qin, Yanlin; Wu, Ruchun; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. RP Yang, DJ (reprint author), S China Univ Technol, Sch Chem & Chem Engn, 381 Wushan Rd, Guangzhou 510641, Guangdong, Peoples R China.; Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM cedjyang@scut.edu.cn; jzhu@fs.fed.us FU USDA-NIFA [2011-68005-30416]; Chinese Scholarship Council (CSC); International Science and Technology Cooperation Program of China (ISTCP) [2013DFA41670]; USDA Forest Products Laboratory (FPL) FX This work was partially supported by a USDA-NIFA coordinated agriculture project to the Northwest Advanced Renewables Alliance (Grant No. 2011-68005-30416), the Chinese Scholarship Council (CSC), and the International Science and Technology Cooperation Program of China (ISTCP) Grant 2013DFA41670. The funding from these programs made the visiting appointment of Qin and Wu at the USDA Forest Products Laboratory (FPL) possible. We would like to acknowledge Jerry Gargulak of LignoTech for providing us the D-748 Lignosulfonate. NR 31 TC 1 Z9 1 U1 17 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD APR PY 2016 VL 4 IS 4 BP 2200 EP 2205 DI 10.1021/acssuschemeng.5b01664 PG 6 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DI5QV UT WOS:000373554600037 ER PT J AU Rusch, A Chaplin-Kramer, R Gardiner, MM Hawro, V Holland, J Landis, D Thies, C Tscharntke, T Weisser, WW Winqvist, C Woltz, M Bommarco, R AF Rusch, Adrien Chaplin-Kramer, Rebecca Gardiner, Mary M. Hawro, Violetta Holland, John Landis, Douglas Thies, Carsten Tscharntke, Teja Weisser, Wolfgang W. Winqvist, Camilla Woltz, Megan Bommarco, Riccardo TI Agricultural landscape simplification reduces natural pest control: A quantitative synthesis SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Crop protection; Biological control; Arthropods intraguild predation; Ecosystem services; Landscape management; Spatial stability ID LAND-USE INTENSITY; BIOLOGICAL-CONTROL; ECOSYSTEM SERVICES; HABITAT MANAGEMENT; ENEMY BIODIVERSITY; WINTER-WHEAT; APHID PREY; CROP PEST; COMPLEXITY; INTENSIFICATION AB Numerous studies show that landscape simplification reduces abundance and diversity of natural enemies in agroecosystems, but its effect on natural pest control remains poorly quantified. Further, natural enemy impacts on pest populations have usually been estimated for a limited number of taxa and have not considered interactions among predator species. In a quantitative synthesis with data collected from several cropping systems in Europe and North America, we analyzed how the level and within-field spatial stability of natural pest control services was related to the simplification of the surrounding landscape. All studies used aphids as a model species and exclusion cages to measure aphid pest control. Landscape simplification was quantified by the proportion of cultivated land within a 1 km radius around each plot. We found a consistent negative effect of landscape simplification on the level of natural pest control, despite interactions among enemies. Average level of pest control was 46% lower in homogeneous landscapes dominated by cultivated land, as compared with more complex landscapes. Landscape simplification did not affect the amount of positive or negative interactions among ground dwelling and vegetation-dwelling predators, or the within-field stability of pest control. Our synthesis demonstrates that agricultural intensification through landscape simplification has negative effects on the level of natural pest control with important implications for management to maintain and enhance ecosystem services in agricultural landscapes. Specifically, preserving and restoring semi-natural habitats emerges as a fundamental first step to maintain and enhance pest control services provided by predatory arthropods to agriculture. (C) 2016 Elsevier B.V. All rights reserved. C1 [Rusch, Adrien] INRA, ISVV, UMR1065, SAVE, F-33883 Villenave Dornon, France. [Rusch, Adrien] Univ Bordeaux, Bordeaux Sci Agro, UMR 1065, SAVE, F-33175 Gradignan, France. [Chaplin-Kramer, Rebecca] Stanford Univ, Woods Inst Environm, Nat Capital Project, 371 Serra Mall, Stanford, CA 94305 USA. [Chaplin-Kramer, Rebecca] Univ Calif Berkeley, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. [Gardiner, Mary M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, 1680 Madison Ave, Wooster, OH 44691 USA. [Hawro, Violetta] Polish Acad Sci, Museum & Inst Zool, PL-00679 Warsaw, Poland. [Holland, John] Game & Wildlife Conservat Trust, Fordingbridge SP6 1EF, Hants, England. [Landis, Douglas] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Thies, Carsten; Tscharntke, Teja] Univ Gottingen, Agroecol, Dept Crop Sci, Grisebachstr 6, D-37077 Gottingen, Germany. [Weisser, Wolfgang W.] Tech Univ Munich, Terr Ecol Res Grp, Dept Ecol & Ecosyst Serv, Ctr Food & Life Sci Weihenstephan, Hans Carl von Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. [Winqvist, Camilla; Bommarco, Riccardo] Swedish Univ Agr Sci, Dept Ecol, S-75007 Uppsala, Sweden. [Woltz, Megan] ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Rusch, A (reprint author), INRA, ISVV, UMR1065, SAVE, F-33883 Villenave Dornon, France.; Rusch, A (reprint author), Univ Bordeaux, Bordeaux Sci Agro, UMR 1065, SAVE, F-33175 Gradignan, France. EM adrien.rusch@bordeaux.inra.fr FU Swedish Research Council FORMAS; European Science Foundation; Research Councils UK Rural Economy and Land Use programme [RES-225-25-0093]; Polish Ministry of Science and Higher Education; USDA AFRI Grant [2007-35302-18272]; NSF Long-Term Ecological Research Program; MSU AgBioResearch; DFG; BMBF; FRB (the French Foundation for Research on Biodiversity); cluster of Excellence COTE FX Funding was provided by the Swedish Research Council FORMAS to RB, and the European Science Foundation to the AGRIPOPES-project to RB, CT, TT, and WWW. Studies conducted in the UK formed part of the "Rebugging the system" project RES-225-25-0093 funded through the Research Councils UK Rural Economy and Land Use programme. VH acknowledges support of the Polish Ministry of Science and Higher Education. DL and MW acknowledge support from the USDA AFRI Grant #2007-35302-18272, the NSF Long-Term Ecological Research Program, and MSU AgBioResearch. TT was supported by DFG and BMBF and WWW was supported by DFG. AR acknowledges support from the FRB (the French Foundation for Research on Biodiversity) and from the cluster of Excellence COTE. NR 48 TC 10 Z9 10 U1 38 U2 84 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD APR 1 PY 2016 VL 221 BP 198 EP 204 DI 10.1016/j.agee.2016.01.039 PG 7 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DI6ZO UT WOS:000373649100021 ER PT J AU Steenwerth, KL Calderon-Orellana, A Hanifin, RC Storm, C McElrone, AJ AF Steenwerth, Kerri L. Calderon-Orellana, Arturo Hanifin, Robert C. Storm, Chris McElrone, Andrew J. TI Effects of Various Vineyard Floor Management Techniques on Weed Community Shifts and Grapevine Water Relations SO AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE LA English DT Article DE legume; no-till; permanent cover crops; soil nitrogen availability; tillage; vineyards ID COVER-CROP; MICROBIOLOGICAL FUNCTION; MEDITERRANEAN VINEYARD; SOIL; NITROGEN; TILLAGE; SYSTEM; DYNAMICS; CULTIVATION; AMMONIUM AB Adoption of permanent cover crops and no-till systems is considered integral to achieving the California state air quality standards regulating airborne particulate matter, as indicated in Senate Bill 656. Imposition of such management techniques in a vineyard could create competition with vines for limited water resources. We evaluated the effects of cover crops that were either tilled or just mowed on vineyard floor composition, weed populations, vine water relations and growth, and fruit composition over three years within a mature commercial Merlot vineyard subjected to deficit irrigation in Lodi, San Joaquin Valley, California. The vineyard floor in this experiment supported resident vegetation that was tilled (standard grower practice), an oat cover crop, or a legume/oat cover crop. The two planted cover crops were either tilled or mowed (i.e., no-till). Biomass of cover crops, weeds, and legumes varied by year and treatment, but consistent effects among treatments were not observed. Weed species composition and cover segregated with the presence or absence of tillage rather than cover crop type and the weed species composition of the resident vegetation was distinct from those in the cover crop treatments. Some treatments, like 'Oats/Legumes + NoTill', 'Oats + NoTill', 'Oats/Legumes + Till', and 'Resident Vegetation + Till' reduced soil water content (circle minus(v)) in at least one of the three shallow soil layers spanning 0 to 30 cm, 30 to 60 cm, and 60 to 100 cm in 2008, and 'Oats/Legumes + NoTill' also dried the two upper layers in 2009, but these differences had no consistent influence on plant water status. Distinctions in circle minus(v) between years were attributed partly to the cessation of rainfall two months earlier in 2008 than in 2009, despite similar total annual quantities. Significant reductions in circle minus(v) imposed by the 'Oats/Legumes + NoTill' treatments reduced vine vegetative growth in two of three years, but these effects did not manifest in yield and fruit composition. Values for the Ravaz index for two of the three years indicate that the vineyard was overcropped for all treatments, but maximizing production in this region is a common practice. Weak competitive effects of the cover crops for water were likely associated with the use of a well-established mature vineyard and demonstrated that these management strategies could be employed to improve air quality to meet California air quality regulations with limited effects on vine water status and production. C1 [Steenwerth, Kerri L.; Calderon-Orellana, Arturo; Hanifin, Robert C.; McElrone, Andrew J.] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA USA. [Calderon-Orellana, Arturo] Univ Chile, Dept Engn & Soils, Santiago, Chile. [Storm, Chris] Vino Farms Inc, Lodi, CA USA. RP McElrone, AJ (reprint author), USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA USA. EM andrew.mcelrone@ars.usda.gov FU USDA-ARS Sustainable Viticulture Production Systems (NP305 Crop Production) [2032-21220-006-00] FX This research was funded by USDA-ARS Sustainable Viticulture Production Systems (Project 2032-21220-006-00, NP305 Crop Production). The authors thank C. Manuck, W. Collatz, and D. Sullivan for technical assistance. NR 51 TC 1 Z9 1 U1 7 U2 18 PU AMER SOC ENOLOGY VITICULTURE PI DAVIS PA PO BOX 1855, DAVIS, CA 95617-1855 USA SN 0002-9254 EI 1943-7749 J9 AM J ENOL VITICULT JI Am. J. Enol. Vitic. PD APR PY 2016 VL 67 IS 2 BP 153 EP 162 DI 10.5344/ajev.2015.15050 PG 10 WC Biotechnology & Applied Microbiology; Food Science & Technology; Horticulture SC Biotechnology & Applied Microbiology; Food Science & Technology; Agriculture GA DI4VO UT WOS:000373497700004 ER PT J AU Maki, CR Monteiro, APA Elmore, SE Tao, S Bernard, JK Harvey, RB Romoser, AA Phillips, TD AF Maki, C. R. Monteiro, A. P. A. Elmore, S. E. Tao, S. Bernard, J. K. Harvey, R. B. Romoser, A. A. Phillips, T. D. TI Calcium montmorillonite clay in dairy feed reduces aflatoxin concentrations in milk without interfering with milk quality, composition or yield SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Aflatoxin; Calcium montmorillonite clay; Milk vitamins; Milk composition; Dairy cow ID HUMAN EXPOSURE; NOVASIL CLAY; ALUMINOSILICATE; RIBOFLAVIN; EFFICACY; THIAMIN; SAMPLES; COWS; B-1 AB This study was designed to determine if a calcium montmorillonite clay (Novasil Plus, NSP), can significantly reduce aflatoxin M-1 (AFM(1)) concentrations in milk without affecting dry matter intake (DMI), milk yield, milk composition, vitamin A, or riboflavin concentrations. The study was designed using 15 lactating dairy cows randomly assigned to one of 5 treatment groups in a 5 x 5 Latin square experimental design. The cows were treated with diets containing AFB(1) at 0 or 121 ppb and three different levels of NSP (0.0, 6.0 and 12.1 g/kg) of the diet. Milk samples were collected and analyzed for AFM(1), milk composition, vitamin A, and riboflavin. In this study, AFM(1) concentrations in milk were significantly reduced by both 6.0 g/kg and 12.1 g/kg NSP treatment levels. The low dose of NSP (6.0 g/kg) resulted in a decrease in the level of AFM(1) in the milk equal to 55%; whereas, the high dose (12.1 g/kg) reduced the level of AFM(1) by 68%; milk composition, vitamin A, and riboflavin concentrations were unaffected by any of the dietary treatments. Additionally, dry matter intake (DMI) and milk yield were not different among treatments. (C) 2016 Elsevier B.V. All rights reserved. C1 [Maki, C. R.; Elmore, S. E.; Romoser, A. A.; Phillips, T. D.] Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Monteiro, A. P. A.; Tao, S.; Bernard, J. K.] Univ Georgia, Coll Agr & Environm Sci, Tifton, GA 31793 USA. [Harvey, R. B.] ARS, USDA, Food & Feed Safety Res, College Stn, TX 77845 USA. [Harvey, R. B.; Phillips, T. D.] Texas A&M AgriLife Res, College Stn, TX 77843 USA. RP Phillips, TD (reprint author), VMA, Bldg 107,MS 4458, College Stn, TX 77843 USA. EM tphillips@cvm.tamu.edu FU Aflatoxin Mitigation Center of Excellence Research Program (National Corn Growers Association) [M1403049] FX This work was supported by funding through the Aflatoxin Mitigation Center of Excellence Research Program (National Corn Growers Association), M1403049 NR 24 TC 2 Z9 2 U1 4 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 EI 1873-2216 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD APR PY 2016 VL 214 BP 130 EP 135 DI 10.1016/j.anifeedsci.2016.02.014 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA DI5PJ UT WOS:000373550800014 ER PT J AU Araud, E DiCaprio, E Ma, YM Lou, FF Gao, Y Kingsley, D Hughes, JH Li, JR AF Araud, Elbashir DiCaprio, Erin Ma, Yuanmei Lou, Fangfei Gao, Yu Kingsley, David Hughes, John H. Li, Jianrong TI Thermal Inactivation of Enteric Viruses and Bioaccumulation of Enteric Foodborne Viruses in Live Oysters (Crassostrea virginica) SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HEPATITIS-A VIRUS; BLOOD GROUP ANTIGENS; HUMAN NOROVIRUS SURROGATES; PORCINE GASTRIC MUCIN; TULANE VIRUS; MURINE NOROVIRUS; HUMAN ROTAVIRUS; UNITED-STATES; GASTROINTESTINAL CELLS; PRESSURE INACTIVATION AB Human enteric viruses are among the main causative agents of shellfish-associated outbreaks. In this study, the kinetics of viral bioaccumulation in live oysters and the heat stabilities of the predominant enteric viruses were determined both in tissue culture and in oyster tissues. A human norovirus (HuNoV) GII.4 strain, HuNoV surrogates (murine norovirus [MNV-1], Tulane virus [TV]), hepatitis A virus (HAV), and human rotavirus (RV) bioaccumulated to high titers within oyster tissues, with different patterns of bioaccumulation for the different viruses. We tested the thermal stability of each virus at 62, 72, and 80 degrees C in culture medium. The viruses can be ranked from the most heat resistant to the least stable as follows: HAV, RV, TV, MNV-1. In addition, we found that oyster tissues provided protection to the viruses during heat treatment. To decipher the mechanism underlying viral inactivation by heat, purified TV was treated at 80 degrees C for increasing time intervals. It was found that the integrity of the viral capsid was disrupted, whereas viral genomic RNA remained intact. Interestingly, heat treatment leading to complete loss of TV infectivity was not sufficient to completely disrupt the receptor binding activity of TV, as determined by the porcine gastric mucin-magnetic bead binding assay. Similarly, HuNoV virus-like particles (VLPs) and a HuNoV GII.4 strain retained some receptor binding ability following heat treatment. Although foodborne viruses have variable heat stability, 80 degrees C for >6 min was sufficient to completely inactivate enteric viruses in oysters, with the exception of HAV. C1 [Araud, Elbashir; DiCaprio, Erin; Ma, Yuanmei; Li, Jianrong] Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA. [Lou, Fangfei] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA. [Hughes, John H.] Ohio State Univ, Dept Mol Virol Immunol & Med Genet, Columbus, OH 43210 USA. [Gao, Yu] Ohio State Univ, Dept Extens, Columbus, OH 43210 USA. [Gao, Yu] Ohio State Univ, South Ctr, Piketon, OH USA. [Kingsley, David] Delaware State Univ, ARS, USDA, Food Safety & Intervent Technol Res Unit,James W, Dover, DE USA. RP Li, JR (reprint author), Ohio State Univ, Dept Vet Biosci, Columbus, OH 43210 USA. EM li.926@osu.edu RI Lou, Fangfei/J-9563-2014 OI Lou, Fangfei/0000-0002-9335-8209 FU USDA | National Institute of Food and Agriculture (NIFA) [2011-68003-30005] FX USDA | National Institute of Food and Agriculture (NIFA) provided funding to Jianrong Li under grant number 2011-68003-30005. NR 70 TC 0 Z9 0 U1 3 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2016 VL 82 IS 7 BP 2086 EP 2099 DI 10.1128/AEM.03573-15 PG 14 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2QV UT WOS:000373342400012 PM 26826225 ER PT J AU Gong, HR Chen, XX Chen, YP Hu, FL Zhang, JL Lin, ZG Yu, JW Zheng, HQ AF Gong, Hong-Ri Chen, Xiu-Xian Chen, Yan Ping Hu, Fu-Liang Zhang, Jiang-Lin Lin, Zhe-Guang Yu, Ji-Wei Zheng, Huo-Qing TI Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID DEFORMED-WING VIRUS; HONEY-BEE VIRUSES; MITES VARROA-DESTRUCTOR; TIME QUANTITATIVE PCR; PREVALENCE; COLONIES; KASHMIR; TRANSMISSION; REPLICATION; CHINA AB Sacbrood virus (SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera. In previous studies, SBV isolates infecting A. cerana (AcSBV) and SBV isolates infecting A. mellifera (AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318 A. mellifera colonies and 64 A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38 A. mellifera colonies and 37 A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated from A. mellifera were clustered with the A. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae of A. mellifera, although there were no obvious signs of the disease, demonstrating the replication of AcSBV in A. mellifera. Our results suggest that AcSBV is able to infect A. mellifera colonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities of A. cerana and A. mellifera to sacbrood disease and is potentially useful for guiding beekeeping practices. C1 [Gong, Hong-Ri; Chen, Xiu-Xian; Hu, Fu-Liang; Zhang, Jiang-Lin; Lin, Zhe-Guang; Yu, Ji-Wei; Zheng, Huo-Qing] Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China. [Chen, Yan Ping] USDA ARS, Bee Res Lab, Beltsville, MD USA. RP Zheng, HQ (reprint author), Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China. EM hqzheng@zju.edu.cn FU Modern Agro-industry Technology Research System [CARS-45]; Science and Technology Department of Zhejiang Province, China [2012C12906-19] FX This work was supported by earmarked funds from the Modern Agro-industry Technology Research System (grant CARS-45) and the Science and Technology Department of Zhejiang Province, China (grant 2012C12906-19). NR 50 TC 1 Z9 1 U1 5 U2 10 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2016 VL 82 IS 8 BP 2256 EP 2262 DI 10.1128/AEM.03292-15 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DI2RB UT WOS:000373343300003 PM 26801569 ER PT J AU Ruiz-Santos, P Pfister, JA Verdes, JM AF Ruiz-Santos, Paul Alan Pfister, James Manuel Verdes, Jose TI Conditioning and aversion to toxic Solanum bonariense ("naranjillo") leaves in calves SO CIENCIA RURAL LA English DT Article DE Solanum bonariense; poisonous plants; conditioned taste aversion; calves ID LOCOWEED OXYTROPIS-SERICEA; TASTE-AVERSIONS; FOOD AVERSIONS; SOCIAL FACILITATION; LITHIUM-CHLORIDE; CEREBELLAR DEGENERATION; CATTLE; SHEEP; LARKSPUR; GOATS AB Solanum bonariense is a perennial poisonous shrub that induces cerebellar cortical degeneration when eaten by cattle. The aim of this research was to outline a protocol to induce a conditioned aversion to this plant. During the pre-conditioning period ten calves (126 +/- 12kg BW) were maintained at half of their normal energy intake with lucerne hay and water ad libitum, to stimulate consumption of S. bonariense. Every two days they were offered 100g of S. bonariense leaves for 5 minutes. Calves began eating the target plant on day 10 and consumed all the plant material on day 12. The conditioning period began after each calf consumed the entire amount of S. bonariense for three consecutive sessions. Five animals were randomly selected for conditioning, and after ingestion of S. bonariense they were dosed by oral gavage with lithium chloride (LiCl) at 200mg kg(-1) BW (treated group), while the other five received a similar volume of water by oral gavage (control group). After 2 doses of LiCl the treated group ate no S. bonariense while the control group consumed the entire 100g. We confirmed that LiCl is a powerful tool to induce conditioned aversions against S. bonariense in calves, which persists for at least 3 months. C1 [Ruiz-Santos, Paul; Manuel Verdes, Jose] Univ Republ UDELAR, Fac Vet, Ave Alberto Lasplaces, Montevideo 11600, Uruguay. [Alan Pfister, James] ARS, USDA, Poisonous Plant Res Lab, Logan, UT USA. RP Verdes, JM (reprint author), Univ Republ UDELAR, Fac Vet, Ave Alberto Lasplaces, Montevideo 11600, Uruguay. EM jmverdes@fvet.edu.uy FU "Comision Sectorial de Investigacion Cientifica de la Universidad de la Republica" (CSIC, UdelaR); "Comision de Investigacion y Desarrollo Cientifico" (Facultad de Veterinaria, UdelaR); "Programa de Desarrollo de las Ciencias Basicas" (PEDECIBA), Uruguay FX The authors gratefully acknowledge Elena de Torres (DVM, MSc), Sebastian Brambillasca (DVM, MSc), and Ricardo Pautassi (PhD) for their contributions. This research was supported by "Comision Sectorial de Investigacion Cientifica de la Universidad de la Republica" (CSIC, UdelaR), "Comision de Investigacion y Desarrollo Cientifico" (Facultad de Veterinaria, UdelaR) and "Programa de Desarrollo de las Ciencias Basicas" (PEDECIBA), Uruguay. JMV is a Researcher Career Member of the "Sistema Nacional de Investigadores" (SNI-ANII), Uruguay. NR 29 TC 0 Z9 0 U1 3 U2 4 PU UNIV FEDERAL SANTA MARIA PI SANTA MARIA PA CIDADE UNIV, BAIRRO CAMOBI, SANTA MARIA, RS 97105-900, BRAZIL SN 0103-8478 EI 1678-4596 J9 CIENC RURAL JI Cienc. Rural PD APR PY 2016 VL 46 IS 4 BP 669 EP 673 DI 10.1590/0103-8478cr20150899 PG 5 WC Agronomy SC Agriculture GA DI7UR UT WOS:000373708000017 ER PT J AU Becker, M Carneiro, FM de Oliveira, LP da Silva, MIV Riet-Correa, F Lee, ST Pescador, CA Nakazato, L Colodel, EM AF Becker, Marciel Carneiro, Faber Monteiro de Oliveira, Leonardo Pintar Vincenzi da Silva, Mayara Inacio Riet-Correa, Franklin Lee, Stephen Thomas Pescador, Caroline Argenta Nakazato, Luciano Colodel, Edson Moleta TI Induction and transfer of resistance to poisoning by Amorimia pubiflora in sheep whith non-toxic dosis of the plant and ruminal content SO CIENCIA RURAL LA English DT Article DE Mascagnia pubiflora; monofluoroacetate; ruminal detoxification; sheep; sudden death; toxic plants ID MONOFLUOROACETATE AB Amorimia pubiflora (Malpighiaceae), which contains sodium monofluoroacetate (MFA) is the main cause of "sudden death" in cattle in the Brazilian state of Mato Grosso. This research investigated the induction of resistance to the poisoning in sheep by the continuous administration of non-toxic doses of the plant and also the possibility to transfer this resistance to other sheep by the transfaunation of ruminal fluid. For this a group of four sheep (G1) received daily doses of 0.5g kg(-1) for 20 days and after an interval of 15 days were challenged with three daily doses of 1g kg(-1) for 3 days. Also the transfer of resistance to A. pubiflora poisoning was evaluated by transfaunation of rumen fluid (100ml for 10 days) from G1 sheep to five sheep (G2), followed by challenge with the dose of 1g kg(-1) for 3 days (G2D2) and after a three-day interval they received a single dose of 3g kg(-1) (G2D3). The degree of resistance was evaluated by monitoring the onset of clinical signs, heart rate, and outcome of the poisoning compared with the control groups, which were challenged with three daily doses of 1g kg(-1) (G3) and with a single dose of 3g kg(-1) (G4). Clinical parameters evaluated in Groups G1 and G2 were significantly less pronounced than those observed in G3 and G4 (control) (P< 0.05). Sheep in G4 (control) died after receiving a single dose of 3g kg(-1), while those in G2 (transfaunated) survived. These findings demonstrated that consumption of non-toxic doses of A. pubiflora induced resistance in sheep and that this resistance can be transferred by transfaunation. New experiments are needed to determine the most efficient ways to induce resistance and to use this technique in the field to prevent the poisoning. C1 [Becker, Marciel; Carneiro, Faber Monteiro] Univ Fed Mato Grosso UFMT, Fac Agron & Med Vet, Programa Posgrad Ciencias Vet, Ave Fernando Correa da Costa 2367, BR-78068900 Cuiaba, MT, Brazil. [de Oliveira, Leonardo Pintar; Vincenzi da Silva, Mayara Inacio] Univ Fed Mato Grosso UFMT, Fac Agron & Med Vet, Dept Med Vet, BR-78068900 Cuiaba, MT, Brazil. [Riet-Correa, Franklin] UFCG, Ctr Saude Tecnol Rural, Hosp Vet, Campus Patos, Patos de Minas, PB, Brazil. [Lee, Stephen Thomas] ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. [Pescador, Caroline Argenta; Nakazato, Luciano; Colodel, Edson Moleta] Univ Fed Mato Grosso UFMT, Fac Agron & Med Vet, Dept Clin Med Vet, BR-78068900 Cuiaba, MT, Brazil. RP Becker, M (reprint author), Univ Fed Mato Grosso UFMT, Fac Agron & Med Vet, Programa Posgrad Ciencias Vet, Ave Fernando Correa da Costa 2367, BR-78068900 Cuiaba, MT, Brazil. EM marcielbecker@hotmail.com FU National Institute of Science and Technology for Poison Control for Plants [0573534-2008-0] FX The National Institute of Science and Technology for Poison Control for Plants - Process 0573534-2008-0 for funding this project, Dr William R. Anderson for botanical identification. NR 17 TC 0 Z9 0 U1 1 U2 1 PU UNIV FEDERAL SANTA MARIA PI SANTA MARIA PA CIDADE UNIV, BAIRRO CAMOBI, SANTA MARIA, RS 97105-900, BRAZIL SN 0103-8478 EI 1678-4596 J9 CIENC RURAL JI Cienc. Rural PD APR PY 2016 VL 46 IS 4 BP 674 EP 680 DI 10.1590/0103-8478cr20141484 PG 7 WC Agronomy SC Agriculture GA DI7UR UT WOS:000373708000018 ER PT J AU Olson, DM Ruberson, JR Andow, DA AF Olson, D. M. Ruberson, J. R. Andow, D. A. TI Relative longevity of adult Nezara viridula in field cages of cotton, peanut, and soybean SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE southern green stink bug; survival; resource suitability; mortality rate; Hemiptera; Pentatomidae ID STINK BUG HETEROPTERA; HERBIVOROUS INSECTS; PENTATOMIDAE; PERFORMANCE; COPULATIONS; PREFERENCE; FECUNDITY; PATTERNS; BEHAVIOR; BODY AB Producers in the southeastern USA face significant crop losses from the stink bugs Nezara viridula (L.), Euschistus servus (Say), and Chinavia hilaris (Say) (all Hemiptera: Pentatomidae). Cotton, peanut, and soybean are major agronomic crops and host plants of stink bugs in the region. We conducted a field plot study to measure the relative longevity of adult, unmated N.viridula males and females caged on peanut, cotton, and soybean to test three hypotheses: (1) differences in mortality are associated with differences in host plant food suitability, (2) mortality rates increase with age, and (3) males have higher mortality than females. Using survival analysis, we found that the sex of the individual did not affect survival rates on any of the three host plants. Survival was significantly higher in cotton and soybean than in peanut. Mortality rates increased with age in peanut, but not in soybean or cotton. The frequency of canopy temperatures above 35 degrees C was higher in peanut than in soybean. Peanut appears to be a less than ideal habitat in terms of canopy temperature and/or food quality for N.viridula adults. Both, cotton and soybean were equally suitable food resources for N.viridula adults prior to maturation of the plants. C1 [Olson, D. M.] USDA ARS, Crop Protect & Management Res Unit, 2747 Davis Rd, Tifton, GA 31793 USA. [Ruberson, J. R.] Univ Georgia, Dept Entomol, Tifton, GA USA. [Andow, D. A.] Univ Minnesota, Dept Entomol, St Paul, MN USA. RP Olson, DM (reprint author), USDA ARS, Crop Protect & Management Res Unit, 2747 Davis Rd, Tifton, GA 31793 USA. EM dawn.olson@ars.usda.gov OI Ruberson, John/0000-0002-4475-8177 FU National Research Initiative of the USDA National Institute of Food and Agriculture [2008-02409] FX We thank Andy Hornbuckle and Brittany Payne for help in the laboratory, greenhouse, and field. This study was supported by the National Research Initiative of the USDA National Institute of Food and Agriculture, grant number 2008-02409. NR 33 TC 0 Z9 0 U1 5 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD APR PY 2016 VL 159 IS 1 BP 30 EP 36 DI 10.1111/eea.12408 PG 7 WC Entomology SC Entomology GA DI4QB UT WOS:000373483300003 ER PT J AU Bixler, RP Johnson, S Emerson, K Nabatchi, T Reuling, M Curtin, C Romolini, M Grove, JM AF Bixler, R. Patrick Johnson, Shawn Emerson, Kirk Nabatchi, Tina Reuling, Melly Curtin, Charles Romolini, Michele Grove, J. Morgan TI Networks and landscapes: a framework for setting goals and evaluating performance at the large landscape scale SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Review ID SOCIAL-ECOLOGICAL SYSTEMS; SECTOR ORGANIZATIONAL NETWORKS; COLLABORATIVE GOVERNANCE; CONTINENTAL SCALES; CONSERVATION; MANAGEMENT; MISMATCHES; OUTCOMES; SUCCESS; TOOLS AB The objective of large landscape conservation is to mitigate complex ecological problems through interventions at multiple and overlapping scales. Implementation requires coordination among a diverse network of individuals and organizations to integrate local-scale conservation activities with broad-scale goals. This requires an understanding of the governance options and how governance regimes achieve objectives or provide performance evaluation across both space and time. However, empirical assessments measuring network-governance performance in large landscape conservation are limited. We describe a well-established large landscape conservation network in North America, the Roundtable on the Crown of the Continent, to explore the application of a social-ecological performance evaluation framework. Systematic approaches to setting goals, tracking progress, and collecting data for feedback can help guide adaptation. Applying the established framework to our case study provides a means of evaluating the effectiveness of network governance in large landscape conservation. C1 [Bixler, R. Patrick] Texas A&M Univ, Inst Renewable Nat Resources, College Stn, TX USA. [Johnson, Shawn] Univ Montana, Ctr Nat Resources & Environm Policy, Missoula, MT 59812 USA. [Emerson, Kirk] Univ Arizona, Sch Govt & Publ Policy, Tucson, AZ USA. [Nabatchi, Tina] Syracuse Univ, Dept Publ Adm & Int Affairs, Syracuse, NY USA. [Reuling, Melly; Curtin, Charles] Ctr Large Landscape Conservat, Bozeman, MT USA. [Romolini, Michele] Loyola Marymount Univ, Ctr Urban Resilience, Los Angeles, CA 90045 USA. [Grove, J. Morgan] USDA Forest Serv, No Res Stn, Baltimore, MD USA. RP Bixler, RP (reprint author), Texas A&M Univ, Inst Renewable Nat Resources, College Stn, TX USA. EM rpbixler@tamu.edu NR 52 TC 3 Z9 3 U1 12 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD APR PY 2016 VL 14 IS 3 BP 145 EP 153 DI 10.1002/fee.1250 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DI8FE UT WOS:000373736000005 ER PT J AU Davies, VF Mafra, R Beltran, A Baranowski, T Lu, AS AF Davies, Vanessa Fernandes Mafra, Rafaella Beltran, Alicia Baranowski, Thomas Lu, Amy Shirong TI Children's Cognitive and Affective Responses About a Narrative Versus a Non-Narrative Cartoon Designed for an Active Videogame SO GAMES FOR HEALTH JOURNAL LA English DT Article ID CHILDHOOD OBESITY PREVENTION; WISHFUL IDENTIFICATION; US CHILDREN; STORY; GAMES; ADOLESCENTS; PERSUASION; CHARACTERS AB Objective: This article presents the results of interviews conducted with children regarding their cognitive and affective responses toward a narrative and a non-narrative cartoon. The findings will be used to further explore the role of a narrative in motivating continued active videogame play. Materials and Methods: Twenty children (8-11 years old of mixed gender) watched two cartoons (narrative and non-narrative) and were subsequently interviewed. A thematic matrix was used to analyze the interviews. Results: The narrative cartoon (n = 11) was only slightly preferred compared with the non-narrative one (n = 9), with little difference among the participants. The theme categories identified during the analyses were plot, characters, and suggestions. The fight scenes were mentioned by the children as a likeable aspect of the narrative cartoon. In the non-narrative cartoon, the vast majority (n = 17) liked the information about physical activity that was provided. The children enjoyed the appearance and personalities of the characters in both cartoons. A discrepancy in the data about the fight scenes (narrative cartoon) and characters (both cartoons) was found among the female participants (i.e., some girls did not like the fight and thought the characters were too aggressive). However, most of the children wanted to see more action in the story, an increase in the number of fight scenes (narrative cartoon), or more information about exercise and examples of exercises they could do (non-narrative cartoon). They also suggested adding a game to the non-narrative cartoon, including more characters, and improving the animation in both cartoons. Conclusions: The children preferred the narrative cartoon because of the story and the fight. Some gender differences were found, which further studies should investigate. C1 [Davies, Vanessa Fernandes] Univ State Sao Paulo, Fac Publ Hlth, Sao Paulo, Brazil. [Mafra, Rafaella] Univ Fed Santa Catarina, Dept Nutr, Florianopolis, SC, Brazil. [Beltran, Alicia; Baranowski, Thomas] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lu, Amy Shirong] Northeastern Univ, Dept Commun Studies, Coll Arts Media & Design, Boston, MA 02115 USA. [Lu, Amy Shirong] Northeastern Univ, Dept Hlth Sci, Bouve Coll Hlth Sci, Boston, MA 02115 USA. [Davies, Vanessa Fernandes] Univ Estado Sao Paulo, Fac Saude Publ, Ave Dr Arnaldo 715, BR-01246904 Sao Paulo, SP, Brazil. RP Davies, VF (reprint author), Univ Estado Sao Paulo, Fac Saude Publ, Ave Dr Arnaldo 715, BR-01246904 Sao Paulo, SP, Brazil. EM va.davies@hotmail.co.uk OI Lu, Amy Shirong/0000-0002-8230-9049 NR 31 TC 0 Z9 0 U1 4 U2 9 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD APR 1 PY 2016 VL 5 IS 2 BP 114 EP 119 DI 10.1089/g4h.2015.0019 PG 6 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA DI7UU UT WOS:000373708300006 ER PT J AU Coe, JA Baum, RL Allstadt, KE Kochevar, BF Schmitt, RG Morgan, ML White, JL Stratton, BT Hayashi, TA Kean, JW AF Coe, Jeffrey A. Baum, Rex L. Allstadt, Kate E. Kochevar, Bernard F., Jr. Schmitt, Robert G. Morgan, Matthew L. White, Jonathan L. Stratton, Benjamin T. Hayashi, Timothy A. Kean, Jason W. TI Rock-avalanche dynamics revealed by large-scale field mapping and seismic signals at a highly mobile avalanche in the West Salt Creek valley, western Colorado SO GEOSPHERE LA English DT Article ID SINGLE-FORCE REPRESENTATION; FLUIDIZED GRANULAR MASSES; MONTAGUTO EARTH FLOW; 3-DIMENSIONAL TERRAIN; DEBRIS AVALANCHE; CATASTROPHIC LANDSLIDE; MOUNTAIN; MODEL; KINEMATICS; ELEMENTS AB On 25 May 2014, a rain-on-snow-induced rock avalanche occurred in the West Salt Creek valley on the northern flank of Grand Mesa in western Colorado (United States). The avalanche mobilized from a preexisting rock slide in the Green River Formation and traveled 4.6 km down the confined valley, killing three people. The avalanche was rare for the contiguous United States because of its large size (54.5 Mm(3)) and high mobility (height/length = 0.14). To understand the avalanche failure sequence, mechanisms, and mobility, we conducted a forensic analysis using large-scale (1: 1000) structural mapping and seismic data. We used high-resolution, unmanned aircraft system imagery as a base for field mapping, and analyzed seismic data from 22 broadband stations (distances <656 km from the rock-slide source area) and one short-period network. We inverted broadband data to derive a time series of forces that the avalanche exerted on the earth and tracked these forces using curves in the avalanche path. Our results revealed that the rock avalanche was a cascade of landslide events, rather than a single massive failure. The sequence began with an early morning landslide/debris flow that started similar to 10 h before the main avalanche. The main avalanche lasted similar to 3.5 min and traveled at average velocities ranging from 15 to 36 m/s. For at least two hours after the avalanche ceased movement, a central, hummock-rich core continued to move slowly. Since 25 May 2014, numerous shallow landslides, rock slides, and rock falls have created new structures and modified avalanche topography. Mobility of the main avalanche and central core was likely enhanced by valley floor material that liquefied from undrained loading by the overriding avalanche. Although the base was likely at least partially liquefied, our mapping indicates that the overriding avalanche internally deformed predominantly by sliding along discrete shear surfaces in material that was nearly dry and had substantial frictional strength. These results indicate that the West Salt Creek avalanche, and probably other long-traveled avalanches, could be modeled as two layers: a thin, liquefied basal layer, and a thicker and stronger overriding layer. C1 [Coe, Jeffrey A.; Baum, Rex L.; Allstadt, Kate E.; Schmitt, Robert G.; Kean, Jason W.] US Geol Survey, Denver Fed Ctr, MS 966, Denver, CO 80225 USA. [Kochevar, Bernard F., Jr.; Hayashi, Timothy A.] Mesa Cty Dept Publ Works, 200 S Spruce St, Grand Junction, CO 81502 USA. [Morgan, Matthew L.; White, Jonathan L.] Colorado Sch Mines, Colorado Geol Survey, 1801 19th St, Golden, CO 80401 USA. [Stratton, Benjamin T.] US Forest Serv, Gunnison Dist Off, 216 N Colorado, Gunnison, CO 81230 USA. RP Coe, JA (reprint author), US Geol Survey, Denver Fed Ctr, MS 966, Denver, CO 80225 USA. EM jcoe@usgs.gov OI Baum, Rex/0000-0001-5337-1970; Kean, Jason/0000-0003-3089-0369; Coe, Jeffrey/0000-0002-0842-9608 FU National Science Foundation [1349572, EAR-1261681]; USGS, Landslide Hazards Program; Advanced National Seismic System (ANSS); State of Utah FX This work was partially supported by the National Science Foundation under award number 1349572. The facilities of Incorporated Research Institutions for Seismology (IRIS) Data Services (Seattle, Washington), and specifically the IRIS Data Management Center, were used for access to waveforms, related metadata, and/or derived products used in this study. IRIS Data Services are funded through the Seismological Facilities for the Advancement of Geoscience and EarthScope (SAGE) Proposal of the National Science Foundation under Cooperative Agreement EAR-1261681. Seismic data accessed through IRIS came from the USArray Transportable Array, the University of Utah Regional/Urban Seismic Network, the Intermountain West Seismic Network, the Arizona Broadband Seismic Network, and the United States National Seismic Network. Additional data from private networks were kindly provided by the North Fork Valley Seismic Network courtesy of Peter Swanson, and the Paradox Valley Seismic Network through the U.S. Bureau of Reclamation Colorado River Basin Salinity Control Project. Data from the Transportable Array network were made freely available as part of the EarthScope USArray facility, operated by IRIS and supported by the National Science Foundation under Cooperative Agreement EAR-1261681. Financial support was provided by the USGS, including the Landslide Hazards Program, and the Advanced National Seismic System (ANSS) and the State of Utah. Intermountain West network data are freely available from the USGS Earthquake Hazards Program. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 102 TC 3 Z9 3 U1 4 U2 9 PU GEOLOGICAL SOC AMER, INC PI BOULDER PA PO BOX 9140, BOULDER, CO 80301-9140 USA SN 1553-040X J9 GEOSPHERE JI Geosphere PD APR PY 2016 VL 12 IS 2 BP 607 EP 631 DI 10.1130/GES01265.1 PG 25 WC Geosciences, Multidisciplinary SC Geology GA DI0ZV UT WOS:000373226700013 ER PT J AU Yu, LJ Zhong, SY Bian, XD Heilman, WE AF Yu, Lejiang Zhong, Shiyuan Bian, Xindi Heilman, Warren E. TI Climatology and trend of wind power resources in China and its surrounding regions: a revisit using Climate Forecast System Reanalysis data SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article DE wind energy; Pacific Decadal Oscillation; wind power resources in China; East Asian summer monsoon ID ASIAN WINTER MONSOON; INTERANNUAL VARIABILITY; GENERATED ELECTRICITY; TIBETAN PLATEAU; ENERGY RESOURCE; ERA-INTERIM; NCEP-CFSR; SURFACE; SPEED; IMPACTS AB The mean climatology, seasonal and interannual variability and trend of wind speeds at the hub height (80m) of modern wind turbines over China and its surrounding regions are revisited using 33-year (1979-2011) wind data from the Climate Forecast System Reanalysis (CFSR) that has many improvements including higher spatial resolution over previous global reanalysis products. Mean 80-m wind speeds are consistently higher over China Seas and the ocean areas than over land, and inside China high winds are found in areas of Inner Mongolia and the Tibetan Plateau. There is a considerable seasonal variability that reflects primarily the influence of East and Southeast Asia Monsoon with generally higher speeds in winter followed by summer, and weaker winds in autumn, followed by spring. There is also a strong interannual variability, and regions of larger amplitude of variability coincide with regions of higher mean winds. A decreasing trend, dominated by a sharp decline beginning in 2005, is seen across China and the surrounding seas in summer and autumn, and the summer trends over land and over ocean appear to be related respectively to the Pacific Decadal Oscillation (PDO) and the East Asian summer monsoon. Trends are not consistent across the region in spring and winter, however, with positive trends over some areas in northeastern and northwestern China, Mongolia and tropical oceans whereas negative trends in other regions. Nearly all areas of China experience mean annual 80-m wind speed less than 6.9ms(-1) (wind power classes of 1-2) except for some areas of Inner Mongolia where mean annual 80-m wind speeds exceed 6.9ms(-1) (Classes 3 or higher, suitable for wind energy development). China Seas and ocean areas generally fall in Class 3 or above, with the Taiwan and Luzon Straights reaching the highest Class 7. C1 [Yu, Lejiang] Nanjing Univ Informat Sci & Technol, Appl Hydrometeorol Res Inst, Nanjing, Peoples R China. [Yu, Lejiang; Zhong, Shiyuan] Michigan State Univ, Dept Geog, 673 Auditorium Rd, E Lansing, MI 48824 USA. [Yu, Lejiang; Zhong, Shiyuan] Michigan State Univ, Ctr Global Change & Earth Observat, E Lansing, MI 48824 USA. [Bian, Xindi; Heilman, Warren E.] USDA Forest Serv, No Res Stn, Lansing, MI USA. RP Zhong, SY (reprint author), Michigan State Univ, Dept Geog, 673 Auditorium Rd, E Lansing, MI 48824 USA. EM zhongs@msu.edu FU National Science Foundation of China [41401017]; Open Foundation of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering [2014490911]; AgBioResearch of Michigan State University FX The authors thank NCEP for producing CFSR data. This research is supported by the National Science Foundation of China (41401017), the Open Foundation of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (2014490911), and by the AgBioResearch of Michigan State University. NR 50 TC 0 Z9 0 U1 7 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 EI 1097-0088 J9 INT J CLIMATOL JI Int. J. Climatol. PD APR PY 2016 VL 36 IS 5 BP 2173 EP 2188 DI 10.1002/joc.4485 PG 16 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DI6MG UT WOS:000373612400003 ER PT J AU Manthey, AK Kalscheur, KF Garcia, AD Mjoun, K AF Manthey, A. K. Kalscheur, K. F. Garcia, A. D. Mjoun, K. TI Lactation performance of dairy cows fed yeast-derived microbial protein in low- and high-forage diets SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE yeast-derived microbial protein; forage-to-concentrate ratio; milk production; dairy cows ID NEUTRAL DETERGENT FIBER; AMINO-ACID UTILIZATION; MILK-YIELD; CONCENTRATE RATIO; DEGRADED PROTEIN; ANIMAL NUTRITION; PARTICLE LENGTH; MAMMARY-GLAND; FEED-INTAKE; RUMINAL PH AB The objective of this study was to investigate the effect of substituting soybean meal products with yeast-derived microbial protein (YMP) on lactation performance in diets containing 2 forage-to-concentrate ratios. Sixteen Holstein cows (4 primiparous and 12 multiparous) were randomly assigned to multiple 4 x 4 Latin squares with a 2 x 2 factorial arrangement of treatments. Diets contained low (LF; 45% of diet DM) or high forage (HF; 65% of diet DM) and YMP at 0 (NYMP) or 2.25% (WYMP) of the diet. The forage mix consisted of 67% corn silage and 33% alfalfa hay on a DM basis. No interactions of forage and YMP were noted for any of the production parameters measured. Feed efficiency (energy-corrected milk/dry matter intake) was greater for cows fed NYMP compared with WYMP. Regardless of the addition of YMP, cows fed LF had greater dry matter intake and produced more milk than cows fed HF. In addition, cows fed LF produced more energy-corrected milk than those fed HF. Milk fat percentage was lower in cows fed LF compared with HF, whereas fat yield was similar between forage concentrations. Fat yield tended to decrease with feeding YMP. Interactions of forage and YMP were observed for propionate concentration, acetate and propionate proportion, and acetate-to-propionate ratio. A tendency for an interaction of forage and YMP was also noted for ruminal pH. Cows fed HF diets had greater ruminal ammonia and butyrate concentrations, as well as proportion of butyrate. Arterial concentrations of Ile, Leu, Met, Thr, and Val were greater in cows fed LF. Cows fed NYMP had greater arterial concentrations of Ile, Lys, Trp, and Val than cows fed WYMP. Substitution of soybean proteins with YMP did not improve performance or feed efficiency of high-producing dairy cows regardless of the forage-to-concentrate ratio of the diet. C1 [Manthey, A. K.; Kalscheur, K. F.; Garcia, A. D.] S Dakota State Univ, Dept Dairy Sci, Brookings, SD 57007 USA. [Mjoun, K.] Alltech Inc, Brookings, SD 57006 USA. [Kalscheur, K. F.] ARS, US Dairy Forage Res Ctr, USDA, 1925 Linden Dr, Madison, WI 53706 USA. [Mjoun, K.] 700 32nd Ave, Brookings, SD 57006 USA. RP Kalscheur, KF (reprint author), S Dakota State Univ, Dept Dairy Sci, Brookings, SD 57007 USA. EM kenneth.kalscheur@ars.usda.gov FU Alltech Inc. (Lexington, KY) FX The authors thank Alltech Inc. (Lexington, KY) for the financial support for this project, the graduate students and farm personnel at South Dakota State University for their help with sampling and animal care, and the research personnel at Alltech (Brookings, South Dakota) for their assistance with laboratory analysis. NR 50 TC 0 Z9 0 U1 5 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD APR PY 2016 VL 99 IS 4 BP 2775 EP 2787 DI 10.3168/jds.2015-10014 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DH7BF UT WOS:000372945500033 PM 26851859 ER PT J AU Zanton, GI Heinrichs, AJ AF Zanton, G. I. Heinrichs, A. J. TI Efficiency and rumen responses in younger and older Holstein heifers limit-fed diets of differing energy density SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy heifer; limit feeding; efficiency ID HIGH-CONCENTRATE RATION; DAIRY HEIFERS; LACTATION PERFORMANCE; NUTRIENT UTILIZATION; NITROGEN-UTILIZATION; DIGESTA KINETICS; FIBER DIGESTION; HIGH-FORAGE; MILK-YIELD; COWS AB The objective of this study was to evaluate the effects of limit feeding diets of different predicted energy density on the efficiency of utilization of feed and nitrogen and rumen responses in younger and older Holstein heifers. Eight rumen-cannulated Holstein heifers (4 heifers beginning at 257 +/- 7 d, hereafter "young," and 4 heifers beginning at 610 +/- 16 d, hereafter "old") were limit-fed high [HED; 2.64 Mcal/kg of dry matter (DM), 15.31% crude protein (CP)] or low (LED; 2.42 Mcal/kg of DM, 14.15% CP) energy density diets according to a 4-period, split-plot Latin square design with 28-d periods. Diets were limit-fed to provide isonitrogenous and isoenergetic intake on a rumen empty body weight (BW) basis at a level predicted to support approximately 800 g/d of average daily gain. During the last 7 d of each period, rumen contents were subsampled over a 24-h period, rumen contents were completely evacuated, and total collection of feces and urine was made over 4 d. Intakes of DM and water were greater for heifers fed LED, although, by design, calculated intake of metabolizable energy did not differ between age groups or diets when expressed relative to rumen empty BW. Rumen pH was lower, ammonia (NH3-N) concentration tended to be higher, and volatile fatty acids (VFA) concentration was not different for HED compared with LED and was unaffected by age group. Rumen content mass was greater for heifers fed LED and for old heifers, so when expressing rumen fermentation responses corrected for this difference in pool size, NH3-N pool size was not different between diets and total moles of VFA in the rumen were greater for heifers fed LED, whereas these pool sizes were greater for old heifers. Total-tract digestibility of potentially digestible neutral detergent fiber (NDF) was greater in heifers fed LED and for young heifers, whereas the fractional;rate of ruminal passage and digestion of NDF were both greater in heifers fed LED. Digestibility of N was greater for heifers fed HED, but was unaffected by age group, whereas the efficiency of N retention was greater for heifers fed HED and for young heifers. Manure output was reduced in heifers fed HED, but the effect was largest in old heifers. Results confirm previous studies in which young heifers utilize N more efficiently than old heifers, primarily through greater efficiency of postabsorptive metabolism. Results also support the concept of limit feeding HED diets as a potential means to reduce manure excretion and increase nitrogen efficiency. C1 [Zanton, G. I.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Heinrichs, A. J.] Penn State Univ, Dept Anim Sci, University Pk, PA 16802 USA. RP Zanton, GI (reprint author), ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM geoffrey.zanton@ars.usda.gov FU Management Systems to Improve the Economic and Environmental Sustainability of Dairy Enterprises [NC-2042] FX This research was a component of and partially funded by funds from NC-2042: Management Systems to Improve the Economic and Environmental Sustainability of Dairy Enterprises. NR 39 TC 1 Z9 1 U1 2 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD APR PY 2016 VL 99 IS 4 BP 2825 EP 2836 DI 10.3168/jds.2015-10316 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DH7BF UT WOS:000372945500037 PM 26805967 ER PT J AU Krueger, LA Reinhardt, TA Beitz, DC Stuart, RL Stabel, JR AF Krueger, L. A. Reinhardt, T. A. Beitz, D. C. Stuart, R. L. Stabel, J. R. TI Effects of fractionated colostrum replacer and vitamins A, D, and E on haptoglobin and clinical health in neonatal Holstein calves challenged with Mycobacterium avium ssp. paratuberculosis SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy calf; colostrum; vitamin; acute phase protein ID RETINOIC ACID; SUBSP PARATUBERCULOSIS; ALPHA-TOCOPHEROL; HOST IMMUNITY; DAIRY CALVES; GROWTH; MILK; PROTEIN; PLASMA; COWS AB Thirty Holstein calves were obtained from 2 dairy farms in central Iowa at birth and randomly assigned to 1 of 6 treatment groups: (1) colostrum deprived (CD), no vitamins; (2) colostrum replacer (CR), no vitamins; (3) CR, vitamin A; (4) CR, vitamin D-3; (5) CR, vitamin E; and (6) CR, vitamins A, D-3, E, with 5 calves per treatment in a 14-d study. Calves were fed pasteurized whole milk (CD) or fractionated colostrum replacer (CR) at birth (d 0) and injected with vitamins according to treatment group. From d 1 through d 14 of the study, all calves were fed pasteurized whole milk (PWM) supplemented with vitamins as assigned. All calves were inoculated with Mycobacterium avium ssp. paratuberculosis on d 1 and 3 of age. Calves fed CR acquired IgG(1) and haptoglobin in serum within 24 h of birth, whereas CD calves did not. The CR-fed calves were 2.5 times less likely to develop scours, and CR calves supplemented with vitamins D-3 and E also demonstrated a decreased incidence of scours. Serum vitamin levels of A, D, and E increased within treatment group by d 7 and 14 of the study. Interestingly, synergistic effects of supplemental vitamins A, D-3, and E on serum 25-(OH)-vitamin D were observed at d 7, resulting in higher levels than in calves administered vitamin D only. Further, vitamin D-3 deficiency was observed in CD and CR calves fed a basal diet of pasteurized whole milk and no supplemental vitamins. Colonization of tissues with Mycobacterium avium, ssp. paratuberculosis was negligible and was not affected by colostrum feeding or vitamin supplementation. Results demonstrated passive transfer of haptoglobin to neonatal calves, and potential health benefits of supplemental vitamins D-3 and E to calves fed pasteurized whole milk. C1 [Krueger, L. A.; Beitz, D. C.; Stabel, J. R.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Krueger, L. A.; Reinhardt, T. A.; Stabel, J. R.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Stuart, R. L.] Stuart Prod Inc, Bedford, TX 76022 USA. RP Stabel, JR (reprint author), ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM judy.stabel@ars.usda.gov FU Stuart Products (Bedford, TX); USDA-Agricultural Research Service FX This study was partially funded by Stuart Products (Bedford, TX). The majority of the funding for this project was provided by the USDA-Agricultural Research Service. The authors thank the following individuals, all from NADC (Ames, IA): Margaret Walker, Randy Hempel, and Derrel Hoy for their help with laboratory assays, and Brian Conrad, Ben Samek, and Hannah Schroeder for their excellent work with the neonatal calves. NR 33 TC 3 Z9 3 U1 8 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD APR PY 2016 VL 99 IS 4 BP 2884 EP 2895 DI 10.3168/jds.2015-10395 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DH7BF UT WOS:000372945500043 PM 26805975 ER PT J AU Chifflet, L Rodriguero, MS Calcaterra, LA Rey, O Dinghi, PA Baccaro, FB Souza, JLP Follett, P Confalonieri, VA AF Chifflet, L. Rodriguero, M. S. Calcaterra, L. A. Rey, O. Dinghi, P. A. Baccaro, F. B. Souza, J. L. P. Follett, P. Confalonieri, V. A. TI Evolutionary history of the little fire ant Wasmannia auropunctata before global invasion: inferring dispersal patterns, niche requirements and past and present distribution within its native range SO JOURNAL OF EVOLUTIONARY BIOLOGY LA English DT Article DE Bayesian phylogeography; biological invasion; invasive ant; mitochondrial DNA; Pleistocene refugia; range expansion; South America; species distribution model ID BRAZILIAN ATLANTIC FOREST; MULTILOCUS GENOTYPE DATA; SOLENOPSIS-INVICTA; INTRODUCED RANGES; GENETIC-STRUCTURE; ARGENTINE ANT; HYMENOPTERA-FORMICIDAE; BAYESIAN-INFERENCE; DNA POLYMORPHISM; POPULATION AB The evolutionary history of invasive species within their native range may involve key processes that allow them to colonize new habitats. Therefore, phylogeographic studies of invasive species within their native ranges are useful to understand invasion biology in an evolutionary context. Here we integrated classical and Bayesian phylogeographic methods using mitochondrial and nuclear DNA markers with a palaeodistribution modelling approach, to infer the phylogeographic history of the invasive ant Wasmannia auropunctata across its native distribution in South America. We discuss our results in the context of the recent establishment of this mostly tropical species in the Mediterranean region. Our Bayesian phylogeographic analysis suggests that the common ancestor of the two main clades of W.auropunctata occurred in central Brazil during the Pliocene. Clade A would have differentiated northward and clade B southward, followed by a secondary contact beginning about 380000years ago in central South America. There were differences in the most suitable habitats among clades when considering three distinct climatic periods, suggesting that genetic differentiation was accompanied by changes in niche requirements, clade A being a tropical lineage and clade B a subtropical and temperate lineage. Only clade B reached more southern latitudes, with a colder climate than that of northern South America. This is concordant with the adaptation of this originally tropical ant species to temperate climates prior to its successful establishment in the Mediterranean region. This study highlights the usefulness of exploring the evolutionary history of invasive species within their native ranges to better understand biological invasions. C1 [Chifflet, L.; Rodriguero, M. S.; Dinghi, P. A.; Confalonieri, V. A.] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Ecol Genet & Evoluc, EGE FCEN UBA, Intendente Guiraldes 2160 C1428EGA,Pabellon 2, Buenos Aires, DF, Argentina. [Chifflet, L.; Rodriguero, M. S.; Dinghi, P. A.; Confalonieri, V. A.] IEGEBA UBA CONICET, Intendente Guiraldes 2160 C1428EGA,Pabellon 2, Buenos Aires, DF, Argentina. [Calcaterra, L. A.] Fdn Estudio Especies Invasivas FUEDEI, Hurlingham, Buenos Aires, Argentina. [Rey, O.] INRA, UMR1062, CBGP, F-34060 Montpellier, France. [Rey, O.] CNRS, USR2936, Stn Ecol Expt, CNRS Moulis, Moulis, France. [Baccaro, F. B.] Univ Fed Amazonas UFAM, Dept Biol, Coroado I, AM, Brazil. [Souza, J. L. P.] INPA, Programa Posgrad Entomol, Manaus, Amazonas, Brazil. [Follett, P.] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI USA. RP Chifflet, L (reprint author), Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Ecol Genet & Evoluc, EGE FCEN UBA, Intendente Guiraldes 2160 C1428EGA,Pabellon 2, Buenos Aires, DF, Argentina.; Chifflet, L (reprint author), IEGEBA UBA CONICET, Intendente Guiraldes 2160 C1428EGA,Pabellon 2, Buenos Aires, DF, Argentina. EM lu_chifflet@hotmail.com RI Souza, Jorge/L-5154-2013; Baccaro, Fabricio/H-3885-2012 OI Souza, Jorge/0000-0003-4574-8111; Baccaro, Fabricio/0000-0003-4747-1857 FU Universidad de Buenos Aires; ANPCyT; CONICET, Argentina; USDA-ARS-Pacific Basin Agricultural Research Center; French Ministere de l'Ecologie et du Developpement Durable (ECOTROP program); French Agropolis foundation (RTRA-Montpellier, BIOFIS) [1001-001] FX We are grateful to Cristian Tomatis and Noelia Guzman for laboratory assistance; to Laura Varone, Priscila Hanisch and Francisco Sola for providing some of the samples; and to John Heraty, Edward Lebrun, Dietrich Gozek and Silvia Pietrokovsky for their helpful comments and suggestions on the manuscript. This research project was supported by grants from Universidad de Buenos Aires, ANPCyT and CONICET, Argentina, and from USDA-ARS-Pacific Basin Agricultural Research Center. OR is also grateful to the French Ministere de l'Ecologie et du Developpement Durable (ECOTROP program) and the French Agropolis foundation (RTRA-Montpellier, BIOFIS project 1001-001) for financial support. NR 120 TC 1 Z9 1 U1 7 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1010-061X EI 1420-9101 J9 J EVOLUTION BIOL JI J. Evol. Biol. PD APR PY 2016 VL 29 IS 4 BP 790 EP 809 DI 10.1111/jeb.12827 PG 20 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA DJ0XZ UT WOS:000373929000011 PM 26780687 ER PT J AU Agga, GE Arthur, TM Schmidt, JW Wang, R Brichta-Harhay, DM AF Agga, Getahun E. Arthur, Terrance M. Schmidt, John W. Wang, Rong Brichta-Harhay, Dayna M. TI Diagnostic Accuracy of Rectoanal Mucosal Swab of Feedlot Cattle for Detection and Enumeration of Salmonella enterica SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Cattle; Concordance; Fecal grab; Rectoanal mucosal swab; Salmonella; Sensitivity ID ESCHERICHIA-COLI O157-H7; BEEF PROCESSING PLANTS; DIRECT PLATING METHODS; IN-GROUND BEEF; UNITED-STATES; ANTIMICROBIAL RESISTANCE; DAIRY OPERATIONS; FECAL CULTURES; PREVALENCE; CONTAMINATION AB Cattle are noted carriers of the foodborne pathogen Salmonella enterica. The perceived need to decrease the potential human health risk posed by excretion of this pathogen has resulted in numerous studies examining the factors that influence Salmonella shedding in cattle. Fecal grab (FG) samples have been the predominant method used to identify cattle colonized or infected with Salmonella; however, FG sampling can be impractical in certain situations, and rectoanal mucosal swabs (RAMS) are a more convenient sample type to collect. Despite a lack of studies comparing FG and RAMS for the detection and enumeration of Salmonella fecal shedding, RAMS is perceived as less sensitive because a smaller amount of feces is cultured. In a cross-sectional study to address these concerns, paired RAMS and FG samples were collected from 403 adult feedlot cattle approximately 90 days prior to harvest. Samples were processed for Salmonella enumeration (direct plating) and detection (enrichment and immunomagnetic separation). In all, 89.6% of RAMS and 98.8% of FG samples were positive for Salmonella, and concordant prevalence outcomes were observed for 90.8% of samples. Mean enumeration values were 3.01 and 3.12 log CFU/ml for RAMS and FG, respectively. The sensitivity and specificity of RAMS were 91% (95% confidence interval [CI]: 87.5 to 93%) and 100% (95% CI: 48 to 100%), respectively, for Salmonella detection. Furthermore, RAMS Salmonella enumeration was substantially concordant (rho(c) = 0.89; 95% CI: 0.86 to 0.91) with FG values. We conclude that RAMS are a reliable alternative to FG for assessing cattle Salmonella fecal shedding status, especially for cattle shedding high levels of Salmonella. C1 [Agga, Getahun E.; Arthur, Terrance M.; Schmidt, John W.; Wang, Rong; Brichta-Harhay, Dayna M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. RP Brichta-Harhay, DM (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM Dayna.Harhay@ars.usda.gov FU Beef Checkoff Program FX The authors thank Kim Kucera, Julie Dyer, Frank Reno, Greg Smith, and Bruce Jasch for their outstanding technical support and Jody Gallagher for administrative assistance. We also thank the cooperating feedyard operators for their kind assistance and support. This project was funded, in part, by The Beef Checkoff Program. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 41 TC 0 Z9 0 U1 3 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD APR PY 2016 VL 79 IS 4 BP 531 EP 537 DI 10.4315/0362-028X.JFP-15-409 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DI7AO UT WOS:000373651700002 PM 27052855 ER PT J AU Kalchayanand, N Koohmaraie, M Wheeler, TL AF Kalchayanand, Norasak Koohmaraie, Mohammad Wheeler, Tommy L. TI Effect of Exposure Time and Organic Matter on Efficacy of Antimicrobial Compounds against Shiga Toxin-Producing Escherichia coli and Salmonella SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Antimicrobial compounds; Non-O157 Shiga toxin-producing Escherichia coli; Salmonella; Shiga toxin-producing Escherichia coli O157:H7 ID FRESH BEEF; AEROBIC-BACTERIA; GROUND-BEEF; CATTLE; PCR; INTERVENTIONS; CARCASSES; O145; DECONTAMINATION; CONTAMINATION AB Several antimicrobial compounds are in commercial meat processing plants for pathogen control on beef carcasses. However, the efficacy of the method used is influenced by a number of factors, such as spray pressure, temperature, type of chemical and concentration, exposure time, method of application, equipment design, and the stage in the process that the method is applied. The objective of this study was to evaluate effectiveness of time of exposure of various antimicrobial compounds against nine strains of Shiga toxin producing Escherichia coli (STEC) and four strains of Salmonella in aqueous antimicrobial solutions with and without organic matter. Non-O157 STEC, STEC O157:H7, and Salmonella were exposed to the following aqueous antimicrobial solutions with or without beef purge for 15, 30, 60, 120, 300, 600, and 1,800 s: (i) 2.5% lactic acid, (ii) 4.0% lactic acid, (iii) 2.5% Beefxide, (iv) 1% Aftec 3000, (v) 200 ppm of peracetic acid, (vi) 300 ppm of hypobromous acid, and (vii) water as a control. In general, increasing exposure time to antimicrobial compounds significantly (P <= 0.05) increased the effectiveness against pathogens tested. In aqueous antimicrobial solutions without organic matter, both peracetic acid and hypobromous acid were the most effective in inactivating populations of STEC and Salmonella, providing at least 5.0-log reductions with exposure for 15 s. However, in antimicrobials containing organic matter, 4.0% lactic acid was the most effective compound in reducing levels of STEC and Salmonella, providing 2- to 3-log reductions with exposure for 15 s. The results of this study indicated that organic matter and exposure time influenced the efficacy of antimicrobial compounds against pathogens, especially with oxidizer compounds. These factors should be considered when choosing an antimicrobial compound for an intervention. C1 [Kalchayanand, Norasak; Wheeler, Tommy L.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. [Koohmaraie, Mohammad] IEH Labs & Consulting Grp, 15300 Bothell Way NE, Lake Forest Pk, WA 98155 USA. RP Kalchayanand, N (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM norasak.kalchayanand@ars.usda.gov NR 39 TC 0 Z9 0 U1 3 U2 7 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD APR PY 2016 VL 79 IS 4 BP 561 EP 568 DI 10.4315/0362-028X.JFP-15-204 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DI7AO UT WOS:000373651700006 PM 27052859 ER PT J AU Blagden, T Schneider, W Melcher, U Daniels, J Fletcher, J AF Blagden, Trenna Schneider, William Melcher, Ulrich Daniels, Jon Fletcher, Jacqueline TI Adaptation and Validation of E-Probe Diagnostic Nucleic Acid Analysis for Detection of Escherichia coli O157:H7 in Metagenomic Data from Complex Food Matrices SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Detection; E-probe diagnostic nucleic acid analysis; Escherichia coli O157:H7; Foodborne pathogens; Next generation sequencing ID PATHOGEN; SEPARATION AB The Centers for Disease Control and Prevention recently emphasized the need for enhanced technologies to use in investigations of outbreaks of foodborne illnesses. To address this need, e-probe diagnostic nucleic acid analysis (EDNA) was adapted and validated as a tool for the rapid, effective identification and characterization of multiple pathogens in a food matrix. In EDNA, unassembled next generation sequencing data sets from food sample metagenomes are queried using pathogen-specific sequences known as electronic probes (e-probes). In this study, the query of mock sequence databases demonstrated the potential of EDNA for the detection of foodborne pathogens. The method was then validated using next generation sequencing data sets created by sequencing the metagenome of alfalfa sprouts inoculated with Escherichia coli O157:H7. Nonspecific hits in the negative control sample indicated the need for additional filtration of the e-probes to enhance specificity. There was no significant difference in the ability of an e-probe to detect the target pathogen based upon the length of the probe set oligonucleotides. The results from the queries of the sample database using E. coli e-probe sets were significantly different from those obtained using random decoy probe sets and exhibited 100% precision. The results support the use of EDNA as a rapid response methodology in foodborne outbreaks and investigations for establishing comprehensive microbial profiles of complex food samples. C1 [Blagden, Trenna; Daniels, Jon; Fletcher, Jacqueline] Oklahoma State Univ, Dept Entomol & Plant Pathol, 127 NRC, Stillwater, OK 74078 USA. [Schneider, William] ARS, USDA, Room 139,Bldg 1301,1301 Ditto Ave, Ft Detrick, MD 21702 USA. [Melcher, Ulrich] Oklahoma State Univ, Dept Biochem & Mol Biol, 246 NRC, Stillwater, OK 74078 USA. RP Blagden, T (reprint author), Oklahoma State Univ, Dept Entomol & Plant Pathol, 127 NRC, Stillwater, OK 74078 USA. EM trenna.bladgen@okstate.edu FU U.S. Department of Agriculture-National Institute of Food and Agriculture Plant Biosecurity Program [2010-85605-20542]; Oklahoma Agricultural Experiment Station [OKL02850]; National Science Foundation [OCI-1126330] FX This article was approved for publication by the Director of the Oklahoma Agricultural Experiment Station. The work was funded by the U.S. Department of Agriculture-National Institute of Food and Agriculture Plant Biosecurity Program (grant 2010-85605-20542) and partially supported by the Oklahoma Agricultural Experiment Station (project OKL02850). Thanks are given to Dr. Stephen Marek (Department of Entomology and Plant Pathology, Oklahoma State University) for providing alfalfa seeds, Dr. Hong Jin Hwang (Biochemistry and Molecular Biology Core Facility, Oklahoma State University) for performing the pyrosequencing of samples in this project, and Dr. Joshua Habiger (Department of Statistics, Oklahoma State University) for providing guidance in the statistical analysis. Some of the computing for this project was performed at the HPC and supported in part through a National Science Foundation grant (OCI-1126330). NR 17 TC 1 Z9 1 U1 4 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD APR PY 2016 VL 79 IS 4 BP 574 EP 581 DI 10.4315/0362-028X.JFP-15-440 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DI7AO UT WOS:000373651700008 PM 27052861 ER PT J AU Luchansky, JB Campano, SG Shoyer, BA Porto-Fett, ACS AF Luchansky, John B. Campano, Stephen G. Shoyer, Bradley A. Porto-Fett, Anna C. S. TI Viability of Listeria monocytogenes on Boneless, Water-Added Hams, Commercially Prepared with and without Food-Grade Chemicals, during Extended Storage at 4 and/or-2.2 degrees C SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Buffered vinegar; Clean label; Freezing; Listeria monocytogenes; Organic acids; Sleeping ham ID VACUUM-SEALED PACKAGES; UNCURED TURKEY BREAST; LAURIC ARGINATE; POTASSIUM LACTATE; SODIUM DIACETATE; DELICATESSEN MEATS; 5-STRAIN MIXTURE; HOME STORAGE; FRANKFURTERS; GROWTH AB Viability of Listeria monocytogenes was monitored during refrigerated (4 degrees C) and/or frozen (i.e., deep chilling at -2.2 degrees C) storage on casing-cooked hams that were commercially prepared with and without potassium lactate and sodium diacetate (1.6%), buffered vinegar (2.2%), buffered vinegar and potassium lactate (1.7%), or a blend of potassium lactate, potassium acetate, and sodium diacetate (1.7%). A portion of these hams were subsequently surface treated with lauric arginate ester (LAE; 44 ppm). In phase I, hams (ca. 3.5 kg each) were sliced (ca. 0.7 cm thick, ca. 100 g), inoculated (ca. 4.0 log CFU per slice), surface treated with LAE, and stored at either 4 degrees C for 120 days or at -2.2 degrees C for 90 days and then at 4 degrees C for an additional 120 days. In phase I, without antimicrobials, the population of L. monocytogenes increased by ca. 5.9 log CFU per slice within 120 days at 4 degrees C; however, pathogen levels increased only slightly (ca. 0.45 log CFU per slice) for hams formulated with potassium lactate and sodium diacetate and decreased by ca. 1.2 log CPU per slice when formulated with the other antimicrobials. For slices held at -2.2 degrees C and then stored at 4 degrees C, but not treated with LAE, L. monocytogenes increased by ca. 4.5 log CFU per slice for controls, whereas when formulated with antimicrobials, pathogen levels decreased by ca. 1.4 to 1.8 log CFU per slice. For product treated with LAE, L. monocytogenes increased by ca. 4.0 log CFU per slice for controls, whereas when formulated with antimicrobials, pathogen levels decreased by ca. 0.9 to 1.9 log CFU per slice. In phase II, whole hams (ca. 1.0 kg each) containing antimicrobials were inoculated (6.8 log CFU per ham) and then stored at -2.2 degrees C for 6 months. Pathogen levels decreased by ca. 2.0 to 3.5 log CFU per ham (without LAE treatment) and by ca. 4.2 to 5.2 log CFU per ham (with application of LAE via Sprayed Lethality in Container) when product was held at -2.2 degrees C. In general, deep chilling hams was listericidal, and inclusion of antimicrobials in the formulation suppressed outgrowth of L. monocytogenes during extended cold storage. C1 [Luchansky, John B.; Shoyer, Bradley A.; Porto-Fett, Anna C. S.] USDA, ARS, Wyndmoor, PA 19038 USA. [Campano, Stephen G.] Hawkins Inc, 2381 Rosegate, Roseville, MN 55113 USA. RP Luchansky, JB (reprint author), USDA, ARS, Wyndmoor, PA 19038 USA. EM john.luchansky@ars.usda.gov NR 37 TC 0 Z9 0 U1 1 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD APR PY 2016 VL 79 IS 4 BP 613 EP 619 DI 10.4315/0362-028X.JFP-15-431 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DI7AO UT WOS:000373651700013 PM 27052866 ER PT J AU Cho, KH Pachepsky, YA Kim, M Pyo, J Park, MH Kim, YM Kim, JW Kim, JH AF Cho, Kyung Hwa Pachepsky, Yakov A. Kim, Minjeong Pyo, JongCheol Park, Mi-Hyun Kim, Young Mo Kim, Jung-Woo Kim, Joon Ha TI Modeling seasonal variability of fecal coliform in natural surface waters using the modified SWAT SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil and water assessment tool; Fecal contamination; Surface water; Bacterial water quality ID ESCHERICHIA-COLI; RIVER WATER; TEMPERATURE-DEPENDENCE; SUNLIGHT INACTIVATION; INDICATOR BACTERIA; SOIL RESPIRATION; URBAN STORMWATER; DIE-OFF; TRANSPORT; SURVIVAL AB Fecal coliforms are indicators of pathogens and thereby, understanding of their fate and transport in surface waters is important to protect drinking water sources and public health. We compiled fecal coliform observations from four different sites in the USA and Korea and found a seasonal variability with a significant connection to temperature levels. In all observations, fecal coliform concentrations were relatively higher in summer and lower during the winter season. This could be explained by the seasonal dominance of growth or die-off of bacteria in soil and in-stream. Existing hydrologic models, however, have limitations in simulating the seasonal variability of fecal coliform. Soil and in-stream bacterial modules of the Soil and Water Assessment Tool (SWAT) model are oversimplified in that they exclude simulations of alternating bacterial growth. This study develops a new bacteria subroutine for the SWAT in an attempt to improve its prediction accuracy. We introduced critical temperatures as a parameter to simulate the onset of bacterial growth/die-off and to reproduce the seasonal variability of bacteria. The module developed in this study will improve modeling for environmental management schemes. (C) 2016 Elsevier B.V. All rights reserved. C1 [Cho, Kyung Hwa; Kim, Minjeong; Pyo, JongCheol] Ulsan Natl Inst Sci & Technol, Sch Urban & Environm Engn, Ulsan 689798, South Korea. [Pachepsky, Yakov A.] USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173,BARC East, Beltsville, MD 20705 USA. [Park, Mi-Hyun] Univ Massachusetts, Dept Civil & Environm Engn, 130 Nat Resources Rd, Amherst, MA 01003 USA. [Kim, Young Mo; Kim, Joon Ha] Gwangju Inst Sci & Technol, Sch Environm Sci & Engn, 261 Cheomdan Gwagiro, Gwangju 500712, South Korea. [Kim, Jung-Woo] Korea Atom Energy Res Inst, Radioact Waste Technol Dev Div, 1045 Daedeok Daero, Daejeon 305353, South Korea. RP Kim, JH (reprint author), Gwangju Inst Sci & Technol, Sch Environm Sci & Engn, 261 Cheomdan Gwagiro, Gwangju 500712, South Korea. EM joonkim@gist.ac.kr OI Pachepsky, Yakov/0000-0003-0232-6090 FU National Research Foundation of Korea (NRF) Grant - Korean Government (MSIP) [NRF-2015R1A5A7037825]; Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2014R1A1A2059680] FX This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MSIP) (No. NRF-2015R1A5A7037825) and also supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1A1A2059680). We thank Dan Line (NCSU), Drew A. Gronewold (NOAA-GLERL), and Mass DCR for sharing their bacteria observations and ideas. NR 73 TC 2 Z9 2 U1 6 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD APR PY 2016 VL 535 BP 377 EP 385 DI 10.1016/j.jhydrol.2016.01.084 PG 9 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DI3TN UT WOS:000373421500032 ER PT J AU Xie, Y Yin, SQ Liu, BY Nearing, MA Zhao, Y AF Xie, Yun Yin, Shui-qing Liu, Bao-yuan Nearing, Mark A. Zhao, Ying TI Models for estimating daily rainfall erosivity in China SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil erosion; Rainfall erosivity; Daily rainfall; USLE; China ID SOIL LOSS EQUATION; R-FACTOR; EROSION INDEX; PRECIPITATION; AUSTRALIA; STORMS; USLE; CLIMATE AB The rainfall erosivity factor (R) represents the multiplication of rainfall energy and maximum 30 min intensity by event (EI30) and year. This rainfall erosivity index is widely used for empirical soil loss prediction. Its calculation, however, requires high temporal resolution rainfall data that are not readily available in many parts of the world. The purpose of this study was to parameterize models suitable for estimating erosivity from daily rainfall data, which are more widely available. One-minute resolution rainfall data recorded in sixteen stations over the eastern water erosion impacted regions of China were analyzed. The R-factor ranged from 781.9 to 8258.5 MJ mm ha(-1) h(-1) y(-1). A total of 5942 erosive events from one-minute resolution rainfall data of ten stations were used to parameterize three models, and 4949 erosive events from the other six stations were used for validation. A threshold of daily rainfall between days classified as erosive and non-erosive was suggested to be 9.7 mm based on these data. Two of the models (I and II) used power law functions that required only daily rainfall totals. Model I used different model coefficients in the cool season (Oct.-Apr.) and warm season (May-Sept.), and Model II was fitted with a sinusoidal curve of seasonal variation. Both Model I and Model II estimated the erosivity index for average annual, yearly, and half-month temporal scales reasonably well, with the symmetric mean absolute percentage error MAPE(sym) ranging from 10.8% to 32.1%. Model II predicted slightly better than Model I. However, the prediction efficiency for the daily erosivity index was limited, with the symmetric mean absolute percentage error being 68.0% (Model I) and 65.7% (Model II) and Nash-Sutcliffe model efficiency being 0.55 (Model I) and 0.57 (Model II). Model III, which used the combination of daily rainfall amount and daily maximum 60-min rainfall, improved predictions significantly, and produced a Nash-Sutcliffe model efficiency for daily erosivity index prediction of 0.93. Thus daily rainfall data was generally sufficient for estimating annual average, yearly, and half-monthly time scales, while sub daily data was needed when estimating daily erosivity values. (C) 2016 Elsevier B.V. All rights reserved. C1 [Xie, Yun; Yin, Shui-qing; Liu, Bao-yuan; Zhao, Ying] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Xie, Yun; Yin, Shui-qing; Liu, Bao-yuan; Zhao, Ying] Beijing Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Nearing, Mark A.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. RP Yin, SQ (reprint author), Xin Jie Kou Wai St, Beijing 100875, Peoples R China. EM yinshuiqing@bnu.edu.cn FU National Natural Science Foundation of China [41301281]; National Basic Research Program of China (973 Program) [2007CB407203]; China Scholarship Council FX The authors would like to thank the Heilongjiang, Shanxi, Shaanxi, Sichuan, Hubei, Fujian, and Yunnan Meteorological Bureaus for supplying rainfall data. This work was supported by National Natural Science Foundation of China (No. 41301281), National Basic Research Program of China (973 Program) (No. 2007CB407203) and the China Scholarship Council. Thanks to Bofu Yu for his help in Model II calibration. Thanks to two reviewers for their valuable comments. The USDA is an equal opportunity employer. NR 46 TC 0 Z9 0 U1 8 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD APR PY 2016 VL 535 BP 547 EP 558 DI 10.1016/j.jhydro1.2016.02.020 PG 12 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DI3TN UT WOS:000373421500046 ER PT J AU Stulberg, MJ Huang, Q AF Stulberg, Michael J. Huang, Qi TI A computer program for fast and easy typing of a partial endoglucanase gene sequence into genospecies and sequevars 1&2 of the Ralstonia solanacearum species complex SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE Ralstonia solanacearum species complex; Genospecies; Phylotype; Sequevar; Endoglucanase; Sequence typing; Quarantine pathogen; Select agent; Computer program ID REAL-TIME; BIOVAR 2; STRAINS; DIVERSITY; ASSAY; PCR; MULTIPLEX AB The phytopathogen Ralstonia solanacearum is a species complex that contains race 3 biovar 2 strains belonging to phylotype IIB sequevars 1 and 2 that are quarantined or select agent pathogens. Recently, the R. solanacearum species complex strains have been reclassified into three genospecies: R. solanacearum, Ralstonia pseudosolanacearum and Ralstonia syzygii. An unidentified R. solanacearum strain is considered a select agent in the US until proven to be a non-race 3 biovar 2 (non-phylotype IIB sequevars 1&2). Currently, sequevars of R. solanacearum species complex strains can only be determined by phylogenetic analysis of a partial endoglucanase (egl) sequence of approximately 700-bp in length. Such analysis, however, requires expert knowledge to properly trim the sequence, to include the correct reference strains, and to interpret the results. By com-. paring GenBank egl sequences of representative R. solanacearum species-complex strains, we identified genospecies- and sequevar 1 and 2-specific single nucleotide polymorphisms (SNPs). We also designed primers to amplify a shorter, 526-bp, egl fragment from R. solanacearum species complex strains for easy sequencing of the amplicon, and to facilitate direct and specific amplification of egl from R. solanacearum-infected plant samples without the need of bacterial isolation. We wrote a computer program (Ralstonia solanacearum typing program) that analyzes a minimum 400-bp user-input egl sequence from a R. solanacearum strain for egl homology and SNP content to determine 1) whether it belongs to the R. solanacearum species complex, 2) if so, to which genospecies, and 3) whether it is of the sequevar type (sequevars 1 and 2) associated with the select agent/quarantined R. solanacearum strain. The program correctly typed all 371 tested egl sequences with known sequevars, obtained either from GenBank or through personal communication. Additionally, the program successfully typed 25 R. solanacearum strains in our collection with no prior sequevar information, as well as 4 strains in infected plant samples, using their partial egl sequences amplified and sequenced with primers designed in this study. The Ralstonia solanacearum typing program does not require expertise or specific knowledge to use, gives results in seconds, and provides data interpretation for the user. The program and primers can help expert or non-expert users to quickly type an unknown R. solanacearum species-complex strain and determine whether it is a highly regulated R. solanacearum strain. The program can also serve as a confirmation method, since it is the only method that can easily and directly determine whether the strain in question is a sequevar 1 or 2 strain of R. solanacearum. Published by Elsevier B.V. C1 [Stulberg, Michael J.; Huang, Qi] ARS, Floral & Nursery Plants Res Unit, USDA, Beltsville, MD USA. [Stulberg, Michael J.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA. RP Huang, Q (reprint author), ARS, Floral & Nursery Plants Res Unit, USDA, Beltsville, MD USA. EM qi.huang@ars.usda.gov FU U.S. Department of Agriculture (USDA); Agricultural Research Service (ARS); Animal and Plant Health Inspection Service; DOE [DE-AC05-06OR23100] FX This research was financially supported by the U.S. Department of Agriculture (USDA), Agricultural Research Service (ARS) and Animal and Plant Health Inspection Service. It was supported in part by an appointment to the ARS Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. We thank Daniel Winograd-Cort for his help in learning computer science. We thank Jason Hong for providing egl sequences and Philippe Prior for giving us insight into R. solanacearum egl phylogenetic analysis. We also thank John Hartung for critical review of our manuscript. NR 23 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD APR PY 2016 VL 123 BP 101 EP 107 DI 10.1016/j.mimet.2016.02.010 PG 7 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DI7BY UT WOS:000373655300015 PM 26876453 ER PT J AU Jang, S Sun, JH Chen, P Lakshman, S Molokin, A Harnly, JM Vinyard, BT Urban, JF Davis, CD Solano-Aguilar, G AF Jang, Saebyeol Sun, Jianghao Chen, Pei Lakshman, Sukla Molokin, Aleksey Harnly, James M. Vinyard, Bryan T. Urban, Joseph F., Jr. Davis, Cindy D. Solano-Aguilar, Gloria TI Flavanol-Enriched Cocoa Powder Alters the Intestinal Microbiota, Tissue and Fluid Metabolite Profiles, and Intestinal Gene Expression in Pigs SO JOURNAL OF NUTRITION LA English DT Article DE cocoa flavanols; porcine model; gut microbiota; metabolites; Toll-like receptors ID TOLL-LIKE RECEPTORS; FECAL MICROBIOTA; IMMUNE-SYSTEM; CECUM MODEL; DISEASE; POLYPHENOLS; HEALTH; ACID; CELL; BIFIDOBACTERIA AB Background: Consumption of cocoa-derived polyphenols has been associated with several health benefits; however, their effects on the intestinal microbiome and related features of host intestinal health are not adequately understood. Objective: The objective of this study was to determine the effects of eating flavanol-enriched cocoa powder on the composition of the gut microbiota, tissue metabolite profiles, and intestinal immune status. Methods: Male pigs (5 mo old, 28 kg mean body weight) were supplemented with 0, 2.5, 10, or 20 g flavanol-enriched cocoa powder/d for 27 d. Metabolites in serum, urine, the proximal colon contents, liver, and adipose tissue; bacterial abundance in the intestinal contents and feces; and intestinal tissue gene expression of inflammatory markers and Toll-like receptors (TLRs) were then determined. Results: O-methyl-epicatechin-glucuronide conjugates dose-dependently increased (P < 0.01) in the urine (35- to 204-fold), serum (6- to 186-fold), and adipose tissue (34- to 1144-fold) of pigs fed cocoa powder. The concentration of 3-hydroxyphenylpropionic acid isomers in urine decreased as the dose of cocoa powder fed to pigs increased (75-85%, P < 0.05). Compared with the unsupplemented pigs, the abundance of Lactobacillus species was greater in the feces (7-fold, P = 0.005) and that of Bifidobacterium species was greater in the proximal colon contents (9-fold, P = 0.01) in pigs fed only 20 or 10 g cocoa powder/d, respectively. Moreover, consumption of cocoa powder reduced TLR9 gene expression in ileal Peyer's patches (67-80%, P < 0.05) and mesenteric lymph nodes (43-71%, P < 0.05) of pigs fed 2.5-20 g cocoa powder/d compared with pigs not supplemented with cocoa powder. Conclusion: This study demonstrates that consumption of cocoa powder by pigs can contribute to gut health by enhancing the abundance of Lactobacillus and Bifidobacterium species and modulating markers of localized intestinal immunity. C1 [Jang, Saebyeol; Lakshman, Sukla; Molokin, Aleksey; Urban, Joseph F., Jr.; Solano-Aguilar, Gloria] USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Northeast Area, Beltsville, MD 20705 USA. [Sun, Jianghao; Chen, Pei; Harnly, James M.] USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Northeast Area, Beltsville, MD 20705 USA. [Vinyard, Bryan T.] USDA, Beltsville Human Nutr Res Ctr, Northeast Area, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Davis, Cindy D.] NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA. RP Solano-Aguilar, G (reprint author), USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Northeast Area, Beltsville, MD 20705 USA. EM Gloria.Solano-Aguilar@ars.usda.gov RI Sun, Jianghao/A-6134-2010 FU USDA project [8040-51530-056-00]; Office of Dietary Supplements at NIH [8040-51530-056-13] FX Supported by USDA project 8040-51530-056-00 and interagency agreement 8040-51530-056-13 with the Office of Dietary Supplements at the NIH. Barry-Callebaut supplied Acticoa. NR 50 TC 2 Z9 2 U1 8 U2 21 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2016 VL 146 IS 4 BP 673 EP 680 DI 10.3945/jn.115.222968 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI3RA UT WOS:000373415000003 PM 26936136 ER PT J AU Kao, CC Cope, JL Hsu, JW Dwarkanath, P Karnes, JM Luna, RA Hollister, EB Thame, MM Kurpad, AV Jahoor, F AF Kao, Christina C. Cope, Julia L. Hsu, Jean W. Dwarkanath, Pratibha Karnes, Jeffrey M. Luna, Ruth A. Hollister, Emily B. Thame, Minerva M. Kurpad, Anura V. Jahoor, Farook TI The Microbiome, Intestinal Function, and Arginine Metabolism of Healthy Indian Women Are Different from Those of American and Jamaican Women SO JOURNAL OF NUTRITION LA English DT Article DE arginine; citrulline; microbiome; intestinal permeability; nonpregnant women ID HUMAN GUT MICROBIOME; NITRIC-OXIDE; PLASMA ARGININE; AMINO-ACID; PERMEABILITY; CHILDREN; CITRULLINE; SEQUENCES; MICE; MALABSORPTION AB Background: Indian women have slower arginine flux during pregnancy compared with American and Jamaican women. Arginine is a semi-essential amino acid that becomes essential during periods of rapid lean tissue deposition. It is synthesized only from citrulline, a nondietary amino acid produced mainly in the gut. The gut is therefore a key site of arginine and citrulline metabolism, and gut microbiota may affect their metabolism. Objective: The objective of this study was to identify differences in the gut microbiota of nonpregnant American, Indian, and Jamaican women and characterize the relations between the gut microbiota, gut function, and citrulline and arginine metabolism. Methods: Thirty healthy American, Indian, and Jamaican women (n = 10/group), aged 28.3 +/- 0.8 y, were infused intravenously with [guanidino-N-15(2)]arginine, [5,5-H-2(2)] citrulline, and [N-15(2)] ornithine and given oral [U-C-13(6)]arginine in the fasting and postprandial states. Fecal bacterial communities were characterized by 16S rRNA gene sequencing. Results: In the fasting state, Indian women had lower citrulline flux than did American and Jamaican women [7.0 +/- 0.4 compared with 9.1 +/- 0.4 and 8.9 +/- 0.2 mu mol . kg fat-free mass (FFM)(-1) . h(-1), P = 0.01] and greater enteral arginine conversion to ornithine than did American women (1.4 6 0.11 compared with 1.0 +/- 0.08 mmol . kg FFM-1 . h(-1), P = 0.04). They also had lower mannitol excretion than American and Jamaican women (154 +/- 37.1 compared with 372 +/- 51.8 and 410 +/- 39.6 mg/6 h, P < 0.01). Three dominant stool community types characterized by increased abundances of the genera Prevotella, Bacteroides, and Bacteroides with Clostridium were identified. Indian women had increased mean relative abundances of Prevotella (42%) compared to American and Jamaican women (7% and < 1%, P = 0.03) which were associated with diet, impaired intestinal absorptive capacity, and arginine flux. Conclusions: These findings suggest that dysregulated intestinal function and a unique gut microbiome may contribute to altered arginine metabolism in Indian women. C1 [Kao, Christina C.; Hsu, Jean W.; Karnes, Jeffrey M.; Jahoor, Farook] Texas Childrens Hosp, Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. [Kao, Christina C.] Texas Childrens Hosp, Baylor Coll Med, Dept Med, Sect Pulm Crit Care & Sleep, Houston, TX 77030 USA. [Cope, Julia L.; Luna, Ruth A.; Hollister, Emily B.] Texas Childrens Hosp, Baylor Coll Med, Dept Pathol & Immunol, Houston, TX 77030 USA. [Cope, Julia L.; Luna, Ruth A.; Hollister, Emily B.] Texas Childrens Hosp, Baylor Coll Med, Dept Pathol, Texas Childrens Microbiome Ctr, Houston, TX 77030 USA. [Dwarkanath, Pratibha; Kurpad, Anura V.] St Johns Natl Acad Hlth Sci, St Johns Res Inst, Bangalore, Karnataka, India. [Thame, Minerva M.] Univ W Indies, Dept Child & Adolescent Hlth, Kingston 7, Jamaica. RP Jahoor, F (reprint author), Texas Childrens Hosp, Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr,Dept Pediat, Houston, TX 77030 USA. EM fjahoor@bcm.edu FU Bill & Melinda Gates Foundation; federal funds from USDA Agricultural Research Service [58-6250-6001] FX Supported by the Bill & Melinda Gates Foundation as well as by federal funds from the USDA Agricultural Research Service under cooperative agreement 58-6250-6001. NR 47 TC 1 Z9 1 U1 4 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2016 VL 146 IS 4 BP 706 EP 713 DI 10.3945/jn.115.227579 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI3RA UT WOS:000373415000007 ER PT J AU Bailey, RL Parker, EA Rhodes, DG Goldman, JD Clemens, JC Moshfegh, AJ Thuppal, SV Weaver, CM AF Bailey, Regan L. Parker, Elizabeth A. Rhodes, Donna G. Goldman, Joseph D. Clemens, John C. Moshfegh, Alanna J. Thuppal, Sowmyanarayanan V. Weaver, Connie M. TI Estimating Sodium and Potassium Intakes and Their Ratio in the American Diet: Data from the 2011-2012 NHANES SO JOURNAL OF NUTRITION LA English DT Article DE sodium; potassium; ratio; NHANES; Na:K; diet ID COMPLEX SURVEY DATA; INTAKE DISTRIBUTIONS; REPORT INSTRUMENTS; NUTRIENT INTAKE; BLOOD-PRESSURE; PUBLIC-HEALTH; RECALL DATA; VALIDATION; NUTRITION; VARIANCE AB Background: The dietary sodium-to-potassium ratio (Na:K) is shown to be more strongly associated with an increased risk of cardiovascular disease (CVD) and CVD-related mortality than either sodium or potassium intake alone. Objective: The aim was to estimate the Na: K in the diet of US adults. Methods: Among US adults from the 2011-2012 NHANES (>= 20 y; 2393 men and 2337 women), the National Cancer Institute method was used to estimate sodium and potassium intakes, Na: K, and the percentage of individuals with Na: K < 1.0 utilizing the complex, stratified, multistage probability cluster sampling design. Results: Overall, women had a significantly lower Na: K than men (mean +/- SE: 1.32 +/- 0.02 compared with 1.45 +/- 0.02). Non-Hispanic whites had a significantly lower Na: K than non-Hispanic blacks and non-Hispanic Asians (1.34 +/- 0.02 compared with 1.54 +/- 0.03 and 1.49 +/- 0.04, respectively). Only 12.2% +/- 1.5% of US adults had a Na: K < 1.0. The Na: K decreased linearly as age increased. Most adults (90% +/- 0.8%) had sodium intakes > 2300 mg/d, whereas < 3% had potassium intakes > 4700 mg/d. Grains and vegetables were among the highest contributors to sodium intakes for adults with Na: K < 1.0, compared with protein foods and grains for those with Na: K >= 1.0. Vegetables and milk and dairy products constituted the primary dietary sources of potassium for individuals with Na: K < 1.0, whereas mixed dishes and protein foods contributed the most potassium for individuals with ratios >= 1.0. Individuals with a Na: K < 1.0 were less likely to consume mixed dishes and condiments and were more likely to consume vegetables, milk and dairy products, and fruit than those with a Na: K >= 1.0. Conclusion: Only about one-tenth of US adults have a Na: K consistent with the WHO guidelines for reduced risk of mortality. Continued efforts to reduce sodium intake in tandem with novel strategies to increase potassium intake are warranted. C1 [Bailey, Regan L.] NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA. [Bailey, Regan L.; Thuppal, Sowmyanarayanan V.; Weaver, Connie M.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. [Parker, Elizabeth A.; Rhodes, Donna G.; Goldman, Joseph D.; Clemens, John C.; Moshfegh, Alanna J.] USDA ARS, Beltsville, MD USA. RP Bailey, RL (reprint author), NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA.; Bailey, RL (reprint author), Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA. EM reganbailey@purdue.edu FU Agricultural Research Service, USDA [58-1950-7-707]; NIH Office of Dietary Supplements; Sodium Committee of the North American Branch of the International Life Sciences Institute FX Supported in part by resources from the Agricultural Research Service, USDA agreement 58-1950-7-707 and the NIH Office of Dietary Supplements. Data generation for this work was funded by the Sodium Committee of the North American Branch of the International Life Sciences Institute. NR 36 TC 2 Z9 2 U1 4 U2 5 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2016 VL 146 IS 4 BP 745 EP 750 DI 10.3945/jn.115.221184 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI3RA UT WOS:000373415000012 ER PT J AU Bigornia, SJ Harris, WS Falcon, LM Ordovas, JM Lai, CQ Tucker, KL AF Bigornia, Sherman J. Harris, William S. Falcon, Luis M. Ordovas, Jose M. Lai, Chao-Qiang Tucker, Katherine L. TI The Omega-3 Index Is Inversely Associated with Depressive Symptoms among Individuals with Elevated Oxidative Stress Biomarkers SO JOURNAL OF NUTRITION LA English DT Article DE omega-3 fatty acids; oxidative stress; depression; APOE; Boston Puerto Rican Health Study ID PUERTO-RICAN ADULTS; NUTRITION EXAMINATION SURVEY; POLYUNSATURATED FATTY-ACIDS; CORONARY-HEART-DISEASE; DOCOSAHEXAENOIC ACID; OMEGA-3-FATTY-ACID LEVELS; CARDIOVASCULAR-DISEASE; EICOSAPENTAENOIC ACID; COGNITIVE FUNCTION; MEXICAN-AMERICANS AB Background: Omega-3 (n-3) fatty acid (FA) consumption is thought to improve depressive symptoms. However, current evidence is limited, and whether this association exists among Puerto Ricans, a population burdened by depression, remains uncertain. Objectives: We examined the association between omega-3 FA biomarkers and depressive symptoms as well as the potential influence of oxidative stress. Methods: Baseline and longitudinal analyses were conducted in the Boston Puerto Rican Health Study (n = 787; participants aged 57 +/- 0.52 y, 73% women). Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentration, a measure of oxidative stress, and erythrocyte FA composition were collected at baseline. We calculated the omega-3 index as the sum of eicosapentaenoic and docosahexaenoic acids, expressed as a percentage of total FAs. Baseline and 2-y depressive symptoms were characterized by using the Center for Epidemiological Studies-Depression Scale (CES-D). Statistical analyses included linear and logistic regression. Results: Urinary 8-OHdG concentration tended to modify the relation between the erythrocyte omega-3 index and baseline CES-D score (P-interaction = 0.10). In stratified analyses, the omega-3 index was inversely associated with CES-D score (beta = -1.74, SE = 0.88; P = 0.02) among those in the top quartile of 8-OHdG concentration but not among those in the lower quartiles. The relation between the omega-3 index and CES-D at 2 y was more clearly modified by 8-OHdG concentration (P-interaction = 0.04), where the omega-3 index was inversely associated with CES-D at 2 y, adjusted for baseline (beta = -1.66, SE = 0.66; P = 0.02), only among those with elevated 8-OHdG concentrations. Among individuals not taking antidepressant medications and in the top tertile of urinary 8-OHdG concentration, the omega-3 index was associated with significantly lower odds of a CES-D score >= 16 at baseline (OR: 0.72; 95% CI: 0.53, 0.96) but not at 2 y (OR: 0.83; 95% CI: 0.60, 1.15). Conclusions: An inverse association between the omega-3 index and depressive symptoms was observed among participants with elevated oxidative stress biomarkers. These data suggest that oxidative stress status may identify those who might benefit from omega-3 FA consumption to improve depressive symptoms. C1 [Bigornia, Sherman J.; Tucker, Katherine L.] Univ Massachusetts, Clin Lab & Nutr Sci, Lowell, MA USA. [Falcon, Luis M.; Lai, Chao-Qiang] Univ Massachusetts, Coll Fine Arts Humanities & Social Sci, Lowell, MA USA. [Harris, William S.] Univ S Dakota, Sanford Sch Med, Dept Med, Sioux Falls, SD USA. [Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Bigornia, SJ (reprint author), Univ Massachusetts, Clin Lab & Nutr Sci, Lowell, MA USA. EM bigornia@bu.edu FU NIH [P01-AG023394, P50-HL105185, R01-AG027087] FX Supported by NIH grants P01-AG023394, P50-HL105185, and R01-AG027087. Health Diagnostic Laboratory, Inc., performed the omega-3 index assays. NR 62 TC 3 Z9 3 U1 5 U2 9 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2016 VL 146 IS 4 BP 758 EP 766 DI 10.3945/jn.115.222562 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI3RA UT WOS:000373415000014 PM 26936135 ER PT J AU Taylor, CL Roseland, JM Coates, PM Pehrsson, PR AF Taylor, Christine L. Roseland, Janet M. Coates, Paul M. Pehrsson, Pamela R. TI The Emerging Issue of 25-Hydroxyvitamin D in Foods SO JOURNAL OF NUTRITION LA English DT Letter C1 [Taylor, Christine L.; Coates, Paul M.] NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA. [Roseland, Janet M.; Pehrsson, Pamela R.] ARS, USDA, Beltsville, MD USA. RP Taylor, CL (reprint author), NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA. EM taylorcl3@od.nih.gov NR 9 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2016 VL 146 IS 4 BP 855 EP 856 DI 10.3945/jn.115.228510 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI3RA UT WOS:000373415000026 PM 27037407 ER PT J AU Brown, EM Taylor, MM Liu, CK AF Brown, Eleanor M. Taylor, Maryann M. Liu, Cheng-Kung TI SOLUBLE COLLAGEN APPROACH TO A COMBINATION TANNAGE MECHANISM SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID POWDERED HIDE MODEL; I COLLAGEN; INDUCED DENATURATION; HYDROLYZABLE TANNIN; NEUTRAL SALTS; ALUMINUM AB Although complex salts of Cr-III sulfate are currently the most effective tanning agents, salts of other metals, including aluminum, have been used either alone or in combination with vegetable tannins or other organic chemicals. In the present study, the interactions of aluminum sulfate, and quebracho or chestnut tannins with collagen were investigated. A model system was devised to use soluble collagen in one compartment of an equilibrium dialysis cell and solutions of mineral or polyphenolic tanning agents in the other compartment. This study, by focusing on the effects of tanning agents on soluble collagen, rather than on intact hide, or powdered hide, gives a somewhat different perspective on the tanning process. The extraction of water from the collagen solution in the formation of aluminum/tannin complexes suggests that the lowering of water activity around the collagen may play a role in stabilizing a collagen/tannin/aluminum tannage. The most interesting finding is that aluminum which has little effect on collagen helical structure and stability does appear to connect collagen molecules in some manner to produce high molecular weight species that do not separate under the conditions of SDS PAGE. Comparison of the interactions of various combinations of minerals and vegetable tannins with collagen is expected to provide insight into a more generalized mechanism for tanning. C1 [Brown, Eleanor M.; Taylor, Maryann M.; Liu, Cheng-Kung] ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid lane, Wyndmoor, PA 19038 USA. RP Brown, EM (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid lane, Wyndmoor, PA 19038 USA. EM eleanor.brown@ars.usda.gov NR 22 TC 0 Z9 0 U1 3 U2 3 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD APR PY 2016 VL 111 IS 4 BP 141 EP 147 PG 7 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA DI7DY UT WOS:000373660500002 ER PT J AU Taylor, MM Bumanlag, LP Brown, EM Liu, CK AF Taylor, M. M. Bumanlag, L. P. Brown, E. M. Liu, C. -K. TI REACTION OF PROTEIN AND CARBOHYDRATES WITH EDC FOR MAKING UNIQUE BIOMATERIALS SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID CROSS-LINKED GELATIN; 1-ETHYL-3-(3-DIMETHYLAMINOPROPYL) CARBODIIMIDE EDC; ENZYMATICALLY MODIFIED GELATIN; OXIDIZED PHENOLIC-COMPOUNDS; WATER-SOLUBLE CARBODIIMIDE; SOYBEAN 11S GLOBULIN; FISH GELATIN; MICROBIAL TRANSGLUTAMINASE; MECHANICAL-PROPERTIES; HYDROLYSIS PRODUCTS AB Prior research, from this laboratory has demonstrated the feasibility of using chemical and enzymatic treatments on protein and carbohydrate waste products for the purpose of making fillers to enhance the properties of leather. These treatments (microbial transglutaminase, genipin, and polyphenols in the form of vegetable tannins), were effective in reacting with gelatins, whey protein concentrate (WPC), and/or chitosan, alone or in combinations, to give products with interesting functional properties. All crosslinkers were either natural products and/or sustainable materials. In our continuing studies of chemoenzymatic methods to crosslink collagen and collagen by-products, we investigated the extensively reported 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), which has been used to crosslink proteins for purpose of making biomaterials. This present study examined the reactivity of various concentrations of EDC with gelatin, chitosan and combinations of both, in the presence and absence of N-hydroxysuccinimide (NHS) at optimal times, and temperatures, and the effect these parameters had on physical properties, molecular weight distribution and free amine content. It was found that both gelatin and chitosan had reactivity with EDC and the physical properties reflected the concentration of both the carbodiimide and gelatin. It was found however that when the gelatin and chitosan were reacted together in the presence of the carbodiimide, the physical properties improved significantly over the protein and carbohydrate when reacted separately, resulting in unique products. This study provides a better understanding of the reactivity of carbodiimide and optimal conditions for developing appropriate products. C1 [Taylor, M. M.; Bumanlag, L. P.; Brown, E. M.; Liu, C. -K.] ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Taylor, MM (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM taylormaryannm@gmail.com NR 55 TC 1 Z9 1 U1 10 U2 11 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD APR PY 2016 VL 111 IS 4 BP 155 EP 164 PG 10 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA DI7DY UT WOS:000373660500004 ER PT J AU Sweitzer, RA Popescu, VD Thompson, CM Purcell, KL Barrett, RH Wengert, GM Gabriel, MW Woods, LW AF Sweitzer, Rick A. Popescu, Viorel D. Thompson, Craig M. Purcell, Kathryn L. Barrett, Reginald H. Wengert, Greta M. Gabriel, Mourad W. Woods, Leslie W. TI Mortality Risks and Limits to Population Growth of Fishers SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE California; cause-specific; disease; fisher; Kaplan-Meier; lambda; mortality; Pekania pennanti; predation; roadkill; rodenticides; Sierra Nevada; vehicle strike ID WILDLIFE-VEHICLE COLLISIONS; SOUTHERN SIERRA-NEVADA; MARTES-PENNANTI; CANINE-DISTEMPER; MOUNTAIN LIONS; BIGHORN SHEEP; HABITAT SELECTION; PEKANIA-PENNANTI; COMPETING RISKS; CALIFORNIA AB Fishers (Pekania pennanti) in the west coast states of Washington, Oregon, and California, USA have not recovered from population declines and the United States Fish and Wildlife Service has proposed options for listing them as threatened. Our objectives were to evaluate differences in survival and mortality risk from natural (e.g., predation, disease, injuries, starvation) and human-linked causes (e.g., rodenticide exposure [toxicants], vehicle strikes). We monitored survival of 232 radio-collared fishers at a northern and southern study site in the Sierra National Forest, California. We retrieved fisher carcasses, and used necropsy examinations to determine causes of death. We estimated survival and mortality rates using the Kaplan-Meier estimator and nonparametric cumulative incidence functions, respectively, and integrated risk-specific survival rates into a Leslie matrix to evaluate how population growth (l) might improve if management can reduce mortality from any of the risks. We determined cause of death for 93 of 121 fishers, and annual survival was 0.69 for all fishers, and 0.72 for female fishers. Mortality rates were 19.5% for predation; 2.5% for disease, injury, and starvation; and 1.9% for toxicant exposure and vehicle strikes. Predation rates were similar between sexes, and relative risk was 51% lower in fall and winter compared to spring and summer. Combined mortality from disease, injuries, starvation, vehicle strikes, and toxicants was 4.4%, and 11 times higher for males than females. We estimated a base lambda at the northern site of 0.96, which had the potential to increase to 1.03 or 1.11 if predation were reduced by 25% or 50%, respectively, whereas lambda could increase to 0.97 in the absence of deaths from all other risks except predation. Predation was the dominant limiting factor in our study population, and was also the most common mortality risk for fishers in the 3 West Coast states (67%), followed by disease, injury, and starvation (12%) and vehicle strikes (8%). Direct mortality from toxicants appeared limited to fishers in California. Our results identified the need for continued and potential expanded restrictions on habitat disturbance in fisher denning habitats. Research is needed on contact rates between larger predators and fishers, including whether fishers are more vulnerable to attack in open forests or along roads used by coursing predators. (C) 2015 The Wildlife Society. C1 [Sweitzer, Rick A.] Great Basin Inst, 16750 Mt Rose Highway, Reno, NV 89511 USA. [Popescu, Viorel D.] Simon Fraser Univ, Earth Ocean Res Grp, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada. [Popescu, Viorel D.] Univ Bucharest, Ctr Environm Res CCMESI, 1 N Balcescu Blvd, Bucharest, Romania. [Thompson, Craig M.; Purcell, Kathryn L.] US Forest Serv, USDA, Pacific Southwest Res Stn, 2081 E Sierra Ave, Fresno, CA 93710 USA. [Barrett, Reginald H.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Wengert, Greta M.; Gabriel, Mourad W.] Integral Ecol Res Ctr, Blue Lake, CA 95525 USA. [Woods, Leslie W.] Calif Anim Hlth & Food Safety Lab Syst, Davis, CA 95616 USA. RP Sweitzer, RA (reprint author), Great Basin Inst, 16750 Mt Rose Highway, Reno, NV 89511 USA. EM sweitzerrick@gmail.com RI Purcell, Kathryn/S-2592-2016 FU U.S. Forest Service Region 5; U.S. Forest Service Pacific Southwest Research Station FX The field effort would not have been possible without help from a dedicated team of staff and volunteers including J. Ashling, S. Bassing, A. Beaudette J. Busiek, A. Cellar, T. Day, Z. Eads, T. Gorman, D. Hardeman, D. Jackson, W. Mitchell, B. Nieles, C. O'Brien, M. Ratchford, J. Ruthven, J. Schneiderman, W. Sicard, T. Thein, S. Vogel, R. Wise, T. Watson, and others on the northern site, and J. Garner, T. Brickly, J. Banaszak, C. Indelicato, N. Hebert, Z. Miller, B. Nichols, S. Rossler, T. Smith, Z. Stoll, G. Watts, and others on the southern site. We thank Forest Service pilots J. Litton, J. Irving, S. Forkel, B. Bulfer, and C. Haney for aviation support and safety on the northern site. Local support at the northern site was facilitated by B. Persson, A. Otto, and A. Lombardo. We also thank K. Jenkins and R. Powell for valuable comments and suggestions that helped improve the manuscript. Research at the northern site was associated with the Sierra Nevada Adaptive Management Project (SNAMP), a joint effort between U.S. Forest Service Region 5, the University of California, U.S. Forest Service Pacific Southwest Research Station, USFWS, California Department of Water Resources, California Department of Fish and Wildlife, California Department of Forestry and Fire Protection, the University of Wisconsin-Madison, and the University of Minnesota, focused on investigating the effects of landscape fuel treatments on Sierra Nevada forest ecosystems. The U.S. Forest Service Region 5 and the U.S. Forest Service Pacific Southwest Research Station funded the majority of the field and laboratory work associated with this effort, and the California Agricultural Experiment Station assisted with the remainder. This is publication no. 43 from the SNAMP. NR 89 TC 0 Z9 0 U1 36 U2 61 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD APR PY 2016 VL 80 IS 3 BP 438 EP 451 DI 10.1002/jwmg.1020 PG 14 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DI4OV UT WOS:000373480100006 ER PT J AU Lehman, CP Rumble, MA Rota, CT Bird, BJ Fogarty, DT Millspaugh, JJ AF Lehman, Chadwick P. Rumble, Mark A. Rota, Christopher T. Bird, Benjamin J. Fogarty, Dillon T. Millspaugh, Joshua J. TI Elk Resource Selection at Parturition Sites, Black Hills, South Dakota SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Black Hills; calving habitat; elk; grasslands; parturition; ponderosa pine; resource selection ID ROCKY-MOUNTAIN ELK; HABITAT SELECTION; WOODLAND CARIBOU; CALVING SEASON; NATIONAL-PARK; FEMALE ELK; DISTURBANCE; MONTANA; ROADS; PREGNANCY AB We studied elk (Cervus canadensis nelsoni) parturition sites at coarse (314-km(2) and 7-km(2)) and fine (0.2-ha) scales in the Black Hills, South Dakota, 2011-2013, following a period of population decline and poor calf recruitment. Our objective was to test whether female elk selected parturition sites across spatial scales in association with forage, terrain ruggedness, road density, or hiding and security cover. At coarse scales in forests and grasslands, female elk selected sites in areas with greater proportions of vegetation communities that provided forage (56-74% of area) and more rugged topography (194-248 m) than found at random. At coarse scales in grasslands, elk selected sites in areas with lower road densities (<= 1.24 km/plot). At the fine scale in forests and grasslands, female elk selected sites in areas with intermediate slope (19%), closer to water (355-610 m), and far from roads (541-791 m). Further, elk in forests and grasslands selected sites with intermediate security cover (50-88 m). We hypothesize elk selected for intermediate rugged terrain at larger scales for security from high road densities and human disturbance, but these areas may have placed elk in riskier environments for puma (Puma concolor) predation. Forest management that maintains open-canopied vegetation communities in less rugged areas and prevents ponderosa pine (Pinus ponderosa) encroachment of meadows to provide forage may be beneficial for elk. Further, elk parturition sites occurred close to roads, particularly on public lands, and agencies should consider road-use restrictions and vegetation buffers beside roads in areas with less rugged terrain, which may provide favorable calving habitat. (C) 2015 The Wildlife Society. C1 [Lehman, Chadwick P.; Fogarty, Dillon T.] South Dakota Game Fish & Parks, 13329 US Highway 16A, Custer, SD 57730 USA. [Rumble, Mark A.] US Forest Serv, Rocky Mt Res Stn, 8221 South Highway 16, Rapid City, SD 57702 USA. [Rota, Christopher T.; Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, 302 ABNR Bldg, Columbia, MO 65211 USA. [Bird, Benjamin J.] US Forest Serv, Rocky Mt Res Stn, 240 West Prospect Rd, Ft Collins, CO 80526 USA. RP Lehman, CP (reprint author), South Dakota Game Fish & Parks, 13329 US Highway 16A, Custer, SD 57730 USA. EM chad.lehman@state.sd.us OI Rota, Christopher/0000-0001-9272-4687 FU Rocky Mountain Elk Foundation; South Dakota Department of Game, Fish and Parks, Federal Aid to Wildlife Restoration Fund [W-75-R-53, 7544] FX Field support was provided by S. R. Lindsey, J. J. Kragel, A. N. Coleman, K. D. Kaskie, N. D. Markl, L. E. Schmitz, T. M. Gingery, M. N. Lenny, G. S. Lenny, L. L. Flack, G. C. Brundige, and K. W. Knudsen. J. D. Raithel and R. C. Cook reviewed drafts of this manuscript. We thank L. S. Baggett for statistical assistance. Additional field assistance and technical support was provided by The U.S. Forest Service Rocky Mountain Research Station and University of Missouri. Funding for this research was provided by the Rocky Mountain Elk Foundation and the South Dakota Department of Game, Fish and Parks, Federal Aid to Wildlife Restoration Fund (Project W-75-R-53, No. 7544). NR 81 TC 1 Z9 1 U1 12 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD APR PY 2016 VL 80 IS 3 BP 465 EP 478 DI 10.1002/jwmg.1017 PG 14 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DI4OV UT WOS:000373480100008 ER PT J AU El-Hattab, AW Emrick, LT Hsu, JW Chanprasert, S Almannai, M Craigen, WJ Jahoor, F Scaglia, F AF El-Hattab, Ayman W. Emrick, Lisa T. Hsu, Jean W. Chanprasert, Sirisak Almannai, Mohammed Craigen, William J. Jahoor, Farook Scaglia, Fernando TI Impaired nitric oxide production in children with MELAS syndrome and the effect of arginine and citrulline supplementation SO MOLECULAR GENETICS AND METABOLISM LA English DT Article DE Stable isotope; Mitochondrial diseases; Stroke-like; Lactic acidosis ID STROKE-LIKE EPISODES; TRANSFER RNALEU(UUR) GENE; LACTIC-ACIDOSIS; MITOCHONDRIAL MYOPATHY; TREATMENT OPTIONS; BLOOD-VESSELS; ENCEPHALOPATHY; MUTATION; METABOLISM; ENZYMES AB Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) syndrome is one of the most frequent maternally inherited mitochondrial disorders. The pathogenesis of this syndrome is not fully understood and believed to result from several interacting mechanisms including impaired mitochondrial energy production, microvasculature angiopathy, and nitric oxide (NO) deficiency. NO deficiency in MELAS syndrome is likely to be multifactorial in origin with the decreased availability of the NO precursors, arginine and citrulline, playing a major role. In this study we used stable isotope infusion techniques to assess NO production in children with MELAS syndrome and healthy pediatric controls. We also assessed the effect of oral arginine and citrulline supplementations on NO production in children with MELAS syndrome. When compared to control subjects, children with MELAS syndrome were found to have lower NO production, arginine flux, plasma arginine, and citrulline flux. In children with MELAS syndrome, arginine supplementation resulted in increased NO production, arginine flux, and arginine concentration. Citrulline supplementation resulted in a greater increase of these parameters. Additionally, citrulline supplementation was associated with a robust increase in citrulline concentration and flux and de novo arginine synthesis rate. The greater effect of citrulline in increasing NO production is due to its greater ability to increase arginine availability particularly in the intracellular compartment in which NO synthesis takes place. This study, which is the first one to assess NO metabolism in children with mitochondrial diseases, adds more evidence to the notion that NO deficiency occurs in MELAS syndrome, suggests a better effect for citrulline because of its greater role as NO precursor, and indicates that impaired NO production occurs in children as well as adults with MELAS syndrome. Thus, the initiation of treatment with NO precursors may be beneficial earlier in life. Controlled clinical trials to assess the therapeutic effects of arginine and citrulline on clinical complications of MELAS syndrome are needed. (C) 2016 Elsevier Inc. All rights reserved. C1 [El-Hattab, Ayman W.; Chanprasert, Sirisak; Almannai, Mohammed; Craigen, William J.; Scaglia, Fernando] Baylor Coll Med, Dept Mol & Human Genet, One Baylor Plaza,MS BCM225, Houston, TX 77030 USA. [El-Hattab, Ayman W.] Tawam Hosp, Dept Pediat, Div Clin Genet & Metab Disorders, Al Ain, U Arab Emirates. [Chanprasert, Sirisak; Almannai, Mohammed; Craigen, William J.; Scaglia, Fernando] Texas Childrens Hosp, Houston, TX 77030 USA. [Hsu, Jean W.; Jahoor, Farook] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Emrick, Lisa T.] Baylor Coll Med, Div Neurol & Dev Neurosci, Houston, TX 77030 USA. RP Scaglia, F (reprint author), Baylor Coll Med, Dept Mol & Human Genet, One Baylor Plaza,MS BCM225, Houston, TX 77030 USA. EM fscaglia@bcm.edu FU NCRR NIH HHS [M01 RR000188]; NIGMS NIH HHS [T32 GM007526] NR 42 TC 1 Z9 1 U1 1 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1096-7192 EI 1096-7206 J9 MOL GENET METAB JI Mol. Genet. Metab. PD APR PY 2016 VL 117 IS 4 BP 407 EP 412 DI 10.1016/j.ymgme.2016.01.010 PG 6 WC Endocrinology & Metabolism; Genetics & Heredity; Medicine, Research & Experimental SC Endocrinology & Metabolism; Genetics & Heredity; Research & Experimental Medicine GA DJ0AW UT WOS:000373864800003 PM 26851065 ER PT J AU Hollender, CA Hadiarto, T Srinivasan, C Scorza, R Dardick, C AF Hollender, Courtney A. Hadiarto, Toto Srinivasan, Chinnathambi Scorza, Ralph Dardick, Chris TI A brachytic dwarfism trait (dw) in peach trees is caused by a nonsense mutation within the gibberellic acid receptor PpeGID1c SO NEW PHYTOLOGIST LA English DT Article DE gibberellic acid; GID1; hormone receptor; peach; plum; Rosaceae; tree size ID ARABIDOPSIS-GAI GENE; 2-OXIDASE GENE; INTERSPECIFIC CROSS; FUNCTIONAL-ANALYSIS; ECTOPIC EXPRESSION; VEGETATIVE GROWTH; DISTINCT ROLES; LINKAGE MAP; GID1; PRUNUS AB Little is known about the genetic factors controlling tree size and shape. Here, we studied the genetic basis for a recessive brachytic dwarfism trait (dw) in peach (Prunus persica) that has little or no effect on fruit development. A sequencing-based mapping strategy positioned dw on the distal end of chromosome 6. Further sequence analysis and fine mapping identified a candidate gene for dw as a nonfunctional allele of the gibberellic acid receptor GID1c. Expression of the two GID1-like genes found in peach, PpeGID1c and PpeGID1b, was analyzed. GID1c was predominantly expressed in actively growing vegetative tissues, whereas GID1b was more highly expressed in reproductive tissues. Silencing of GID1c in plum via transgenic expression of a hairpin construct led to a dwarf phenotype similar to that of dw/dw peaches. In general, the degree of GID1c silencing corresponded to the degree of dwarfing. The results suggest that PpeGID1c serves a primary role in vegetative growth and elongation, whereas GID1b probably functions to regulate gibberellic acid perception in reproductive organs. Modification of GID1c expression could provide a rational approach to control tree size without impairing fruit development. C1 [Hollender, Courtney A.; Srinivasan, Chinnathambi; Scorza, Ralph; Dardick, Chris] USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Hadiarto, Toto] BB Biogen, Jl Tentara Pelajar 3A, Bogor, Indonesia. RP Dardick, C (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM chris.dardick@ars.usda.gov FU Agriculture and Food Research Initiative Competitive grant from the USDA National Institute of Food and Agriculture [10891264] FX The authors would like to acknowledge Mark Demuth for generating and maintaining the peach tree population, Michael Whitaker for assistance with cloning, Elizabeth Lutton for screening HRM markers and Emma Acly for assistance with tree measurements. This work was supported by Agriculture and Food Research Initiative Competitive grant 10891264 from the USDA National Institute of Food and Agriculture. NR 70 TC 1 Z9 1 U1 4 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD APR PY 2016 VL 210 IS 1 BP 227 EP 239 DI 10.1111/nph.13772 PG 13 WC Plant Sciences SC Plant Sciences GA DI3ED UT WOS:000373380300027 PM 26639453 ER PT J AU Chitwood, DH Klein, LL O'Hanlon, R Chacko, S Greg, M Kitchen, C Miller, AJ Londo, JP AF Chitwood, Daniel H. Klein, Laura L. O'Hanlon, Regan Chacko, Steven Greg, Matthew Kitchen, Cassandra Miller, Allison J. Londo, Jason P. TI Latent developmental and evolutionary shapes embedded within the grapevine leaf SO NEW PHYTOLOGIST LA English DT Article DE development; grape (Vitis vinifera); leaf morphology; leaf shape; phenotype ID NATURAL VARIATION; GENETIC-BASIS; PHASE-CHANGE; LEAVES; PLANTS; CUCURBITACEAE; HETEROCHRONY; ARCHITECTURE; ASSOCIATION; ARABIDOPSIS AB Across plants, leaves exhibit profound diversity in shape. As a single leaf expands, its shape is in constant flux. Plants may also produce leaves with different shapes at successive nodes. In addition, leaf shape varies among individuals, populations and species as a result of evolutionary processes and environmental influences. Because leaf shape can vary in many different ways, theoretically, the effects of distinct developmental and evolutionary processes are separable, even within the shape of a single leaf. Here, we measured the shapes of > 3200 leaves representing > 270 vines from wild relatives of domesticated grape (Vitis spp.) to determine whether leaf shapes attributable to genetics and development are separable from each other. We isolated latent shapes (multivariate signatures that vary independently from each other) embedded within the overall shape of leaves. These latent shapes can predict developmental stages independent from species identity and vice versa. Shapes predictive of development were then used to stage leaves from 1200 varieties of domesticated grape (Vitis vinifera), revealing that changes in timing underlie leaf shape diversity. Our results indicate that distinct latent shapes combine to produce a composite morphology in leaves, and that developmental and evolutionary contributions to shape vary independently from each other. C1 [Chitwood, Daniel H.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Klein, Laura L.; O'Hanlon, Regan; Chacko, Steven; Greg, Matthew; Kitchen, Cassandra; Miller, Allison J.] St Louis Univ, Dept Biol, St Louis, MO 63103 USA. [Londo, Jason P.] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14456 USA. RP Chitwood, DH (reprint author), Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. EM dchitwood@danforthcenter.org RI Chitwood, Daniel/M-2841-2013 OI Chitwood, Daniel/0000-0003-4875-1447 FU Grape Research Coordination Network [DBI0741876] FX This work was funded by research stipends awarded to D.H.C., L.L.K. and J.P.L. through the Grape Research Coordination Network, DBI0741876. NR 71 TC 7 Z9 7 U1 5 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD APR PY 2016 VL 210 IS 1 BP 343 EP 355 DI 10.1111/nph.13754 PG 13 WC Plant Sciences SC Plant Sciences GA DI3ED UT WOS:000373380300036 PM 26580864 ER PT J AU Daubenmier, J Moran, PJ Kristeller, J Acree, M Bacchetti, P Kemeny, ME Dallman, M Lustig, RH Grunfeld, C Nixon, DF Milush, JM Goldman, V Laraia, B Laugero, KD Woodhouse, L Epel, ES Hecht, FM AF Daubenmier, Jennifer Moran, Patricia J. Kristeller, Jean Acree, Michael Bacchetti, Peter Kemeny, Margaret E. Dallman, Mary Lustig, Robert H. Grunfeld, Carl Nixon, Douglas F. Milush, Jeffrey M. Goldman, Veronica Laraia, Barbara Laugero, Kevin D. Woodhouse, Leslie Epel, Elissa S. Hecht, Frederick M. TI Effects of a mindfulness-based weight loss intervention in adults with obesity: A randomized clinical trial SO OBESITY LA English DT Article ID METABOLIC SYNDROME; DIABETES-MELLITUS; HEART-DISEASE; LIFE-STYLE; MORTALITY; STRESS; METAANALYSIS; INDIVIDUALS; IMPACT; INDEX AB ObjectiveTo determine whether adding mindfulness-based eating and stress management practices to a diet-exercise program improves weight loss and metabolic syndrome components. MethodsIn this study 194 adults with obesity were randomized to a 5.5-month program with or without mindfulness training and identical diet-exercise guidelines. Intention-to-treat analyses with multiple imputation were used for missing data. The primary outcome was 18-month weight change. ResultsEstimated effects comparing the mindfulness to control arm favored the mindfulness arm in (a) weight loss at 12 months, -1.9 kg (95% CI: -4.5, 0.8; P=0.17), and 18 months, -1.7 kg (95% CI: -4.7, 1.2; P=0.24), though not statistically significant; (b) changes in fasting glucose at 12 months, -3.1 mg/dl (95% CI: -6.3, 0.1; P=0.06), and 18 months, -4.1 mg/dl (95% CI: -7.3, -0.9; P=0.01); and (c) changes in triglyceride/HDL ratio at 12 months, -0.57 (95% CI: -0.95, -0.18; P=0.004), and 18 months, -0.36 (95% CI: -0.74, 0.03; P=0.07). Estimates for other metabolic risk factors were not statistically significant, including waist circumference, blood pressure, and C-reactive protein. ConclusionsMindfulness enhancements to a diet-exercise program did not show substantial weight loss benefit but may promote long-term improvement in some aspects of metabolic health in obesity that requires further study. C1 [Daubenmier, Jennifer; Moran, Patricia J.; Acree, Michael; Goldman, Veronica; Hecht, Frederick M.] Univ Calif San Francisco, Osher Ctr Integrat Med, San Francisco, CA 94143 USA. [Daubenmier, Jennifer; Dallman, Mary; Grunfeld, Carl; Laraia, Barbara; Epel, Elissa S.; Hecht, Frederick M.] Univ Calif San Francisco, Ctr Obes Assessment Study & Treatment, San Francisco, CA 94143 USA. [Daubenmier, Jennifer; Grunfeld, Carl; Milush, Jeffrey M.; Hecht, Frederick M.] Univ Calif San Francisco, Dept Med, San Francisco, CA USA. [Kristeller, Jean] Indiana State Univ, Dept Psychol, Terre Haute, IN 47809 USA. [Bacchetti, Peter] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA. [Kemeny, Margaret E.] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA USA. [Dallman, Mary; Epel, Elissa S.] Univ Calif San Francisco, Dept Physiol, Box 0444, San Francisco, CA USA. [Lustig, Robert H.] Univ Calif San Francisco, Dept Pediat, San Francisco, CA USA. [Grunfeld, Carl] Vet Affairs Med Ctr, San Francisco, CA 94121 USA. [Nixon, Douglas F.] Univ Calif San Francisco, Div Expt Med, San Francisco, CA 94143 USA. [Nixon, Douglas F.] George Washington Univ, Dept Microbiol Immunol & Trop Med, Washington, DC USA. [Laraia, Barbara] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA. [Laugero, Kevin D.; Woodhouse, Leslie] USDA, Western Human Nutr Res Ctr, Davis, CA USA. RP Daubenmier, J; Hecht, FM (reprint author), Univ Calif San Francisco, Osher Ctr Integrat Med, San Francisco, CA 94143 USA.; Daubenmier, J; Hecht, FM (reprint author), Univ Calif San Francisco, Ctr Obes Assessment Study & Treatment, San Francisco, CA 94143 USA.; Daubenmier, J; Hecht, FM (reprint author), Univ Calif San Francisco, Dept Med, San Francisco, CA USA. EM jennifer.daubenmier@ucsf.edu; rick.hecht@ucsf.edu FU NIH grants from the National Center for Complementary and Alternative Medicine (NCCAM) [P01AT005013, K24AT007827, K01AT004199]; National Center for Advancing Translational Sciences, UCSF-CTSI [UL1 TR000004] FX This study was supported by NIH grants from the National Center for Complementary and Alternative Medicine (NCCAM) P01AT005013 (Hecht), K24AT007827 (Hecht), and K01AT004199 (Daubenmier), as well as the National Center for Advancing Translational Sciences, UCSF-CTSI Grant Number UL1 TR000004. NR 36 TC 3 Z9 3 U1 10 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD APR PY 2016 VL 24 IS 4 BP 794 EP 804 DI 10.1002/oby.21396 PG 11 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA DI6MM UT WOS:000373613200008 PM 26955895 ER PT J AU Celis-Morales, C Marsaux, CFM Livingstone, KM Navas-Carretero, S San-Cristobal, R O'donovan, CB Forster, H Woolhead, C Fallaize, R Macready, AL Kolossa, S Hallmann, J Tsirigoti, L Lambrinou, CP Moschonis, G Godlewska, M Surwillo, A Grimaldi, K Bouwman, J Manios, Y Traczyk, I Drevon, CA Parnell, LD Daniel, H Gibney, ER Brennan, L Walsh, MC Gibney, M Lovegrove, JA Martinez, JA Saris, WHM Mathers, JC AF Celis-Morales, Carlos Marsaux, Cyril F. M. Livingstone, Katherine M. Navas-Carretero, Santiago San-Cristobal, Rodrigo O'donovan, Clare B. Forster, Hannah Woolhead, Clara Fallaize, Rosalind Macready, Anna L. Kolossa, Silvia Hallmann, Jacqueline Tsirigoti, Lydia Lambrinou, Christina P. Moschonis, George Godlewska, Magdalena Surwillo, Agnieszka Grimaldi, Keith Bouwman, Jildau Manios, Yannis Traczyk, Iwona Drevon, Christian A. Parnell, Laurence D. Daniel, Hannelore Gibney, Eileen R. Brennan, Lorraine Walsh, Marianne C. Gibney, Mike Lovegrove, Julie A. Alfredo Martinez, J. Saris, Wim H. M. Mathers, John C. CA Food4Me Study TI Physical activity attenuates the effect of the FTO genotype on obesity traits in European adults: The Food4Me study SO OBESITY LA English DT Article ID BODY-MASS INDEX; GENE-ENVIRONMENT INTERACTIONS; MISSING HERITABILITY; MEASUREMENT ERROR; ADIPOSITY; CHILDHOOD; VARIANT AB ObjectiveTo examine whether the effect of FTO loci on obesity-related traits could be modified by physical activity (PA) levels in European adults. MethodsOf 1,607 Food4Me participants randomized, 1,280 were genotyped for FTO (rs9939609) and had available PA data. PA was measured objectively using accelerometers (TracmorD, Philips), whereas anthropometric measures [BMI and waist circumference (WC)] were self-reported via the Internet. ResultsFTO genotype was associated with a higher body weight [: 1.09 kg per risk allele, (95% CI: 0.14-2.04), P=0.024], BMI [: 0.54 kgm(-2), (0.23-0.83), P<0.0001], and WC [: 1.07 cm, (0.24-1.90), P=0.011]. Moderate-equivalent PA attenuated the effect of FTO on BMI (P-[interaction]=0.020). Among inactive individuals, FTO increased BMI by 1.06 kgm(-2) per allele (P=0.024), whereas the increase in BMI was substantially attenuated among active individuals (0.16 kgm(-2), P=0.388). We observed similar effects for WC (P-[interaction]=0.005): the FTO risk allele increased WC by 2.72 cm per allele among inactive individuals but by only 0.49 cm in active individuals. ConclusionsPA attenuates the effect of FTO genotype on BMI and WC. This may have important public health implications because genetic susceptibility to obesity in the presence of FTO variants may be reduced by adopting a physically active lifestyle. C1 [Celis-Morales, Carlos; Livingstone, Katherine M.; Mathers, John C.] Newcastle Univ, Inst Cellular Med, Human Nutr Res Ctr, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Marsaux, Cyril F. M.; Saris, Wim H. M.] Maastricht Univ, Med Ctr, Sch Nutr & Translat Res Metab, Dept Human Biol,NUTRIM, NL-6200 MD Maastricht, Netherlands. [Navas-Carretero, Santiago; San-Cristobal, Rodrigo; Alfredo Martinez, J.] Univ Navarra, Dept Nutr Food Sci & Physiol, Pamploma, Spain. [Navas-Carretero, Santiago; San-Cristobal, Rodrigo; Walsh, Marianne C.; Alfredo Martinez, J.] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBERobn, Madrid, Spain. [O'donovan, Clare B.; Forster, Hannah; Woolhead, Clara; Gibney, Eileen R.; Brennan, Lorraine; Gibney, Mike] Univ Coll Dublin, Inst Food & Hlth, Dublin 2, Ireland. [Fallaize, Rosalind; Macready, Anna L.; Lovegrove, Julie A.] Univ Reading, Hugh Sinclair Unit Human Nutr, Reading, Berks, England. [Fallaize, Rosalind; Macready, Anna L.; Lovegrove, Julie A.] Univ Reading, Inst Cardiovasc & Metab Res, Reading, Berks, England. [Kolossa, Silvia; Hallmann, Jacqueline; Daniel, Hannelore] Tech Univ Munich, ZIEL Res Ctr Nutr & Food Sci, Biochem Unit, D-80290 Munich, Germany. [Tsirigoti, Lydia; Lambrinou, Christina P.; Moschonis, George; Manios, Yannis] Harokopio Univ, Dept Nutr & Dietet, Athens, Greece. [Godlewska, Magdalena; Surwillo, Agnieszka; Traczyk, Iwona] Natl Food & Nutr Inst IZZ, Warsaw, Poland. [Grimaldi, Keith] Eurogenetica Ltd, Burnham On Sea, England. [Bouwman, Jildau] TNO, Microbiol & Syst Biol, NL-3700 AJ Zeist, Netherlands. [Drevon, Christian A.] Univ Oslo, Fac Med, Dept Nutr, Inst Basic Med Sci, Oslo, Norway. [Parnell, Laurence D.] Tufts Univ, Nutr & Genom Lab, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA USA. RP Mathers, JC (reprint author), Newcastle Univ, Inst Cellular Med, Human Nutr Res Ctr, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. EM john.mathers@newcastle.ac.uk RI Livingstone, Katherine/R-7202-2016; OI Livingstone, Katherine/0000-0002-9682-7541; Navas-Carretero, Santiago/0000-0002-5163-2230; Mathers, John/0000-0003-3406-3002 FU European Commission [265494] FX This work was supported by the European Commission under the Food, Agriculture, Fisheries and Biotechnology Theme of the 7th Framework Programme for Research and Technological Development [265494]. NR 40 TC 1 Z9 1 U1 4 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD APR PY 2016 VL 24 IS 4 BP 962 EP 969 DI 10.1002/oby.21422 PG 8 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA DI6MM UT WOS:000373613200029 PM 26921105 ER PT J AU Misra, D Fielding, R Felson, D Nevitt, M Torner, J Lewis, CE Neogi, T AF Misra, D. Fielding, R. Felson, D. Nevitt, M. Torner, J. Lewis, C. E. Neogi, T. TI OBESITY,SARCOPENIC OBESITY OR SARCOPENIA: WHICH IS A GREATER RISK FOR KNEE OA? SO OSTEOARTHRITIS AND CARTILAGE LA English DT Meeting Abstract CT World Congress of the Osteoarthritis-Research-Society-International (OARSI) on Osteoarthritis CY MAR 31-APR 03, 2016 CL Amsterdam, NETHERLANDS SP Osteoarthritis Res Soc Int C1 [Misra, D.; Felson, D.; Neogi, T.] Boston Univ, Sch Med, Boston, MA 02118 USA. [Fielding, R.] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Nevitt, M.] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Torner, J.] Univ Iowa, Iowa City, IA USA. [Lewis, C. E.] Univ Alabama Birmingham, Birmingham, AL USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1063-4584 EI 1522-9653 J9 OSTEOARTHR CARTILAGE JI Osteoarthritis Cartilage PD APR PY 2016 VL 24 SU S MA 28 BP S23 EP S24 PG 2 WC Orthopedics; Rheumatology SC Orthopedics; Rheumatology GA DI5KW UT WOS:000373538800058 ER PT J AU Roberts, M Strauch, AM Wiegner, T Mackenzie, RA AF Roberts, Megan Strauch, Ayron M. Wiegner, Tracy Mackenzie, Richard A. TI Leaf Litter Breakdown of Native and Exotic Tree Species in Two Hawaiian Streams that Differ in Flow SO PACIFIC SCIENCE LA English DT Article ID DISSOLVED ORGANIC-CARBON; NITROGEN-FIXING TREE; TROPICAL STREAMS; FOOD WEBS; NUTRIENT DYNAMICS; N-2-FIXING TREE; FOREST STREAM; WATER-QUALITY; RAIN-FORESTS; RIVER AB Riparian leaf litter is a major source of allochthonous organic material to temperate and tropical streams, promoting primary and secondary productivity in lotic and nearshore habitats. In tropical island streams, where native leaf-shredding macroinvertebrates are absent, physical fragmentation from stream flow is an important factor affecting leaf litter breakdown and, thus, organic matter dynamics. Additionally, the invasion of exotic plants into riparian areas is expected to affect litter composition and, consequently, its degradation. We compared the interactions of stream flow and inputs of leaf litter from native and exotic plants on leaf litter breakdown in two streams of varying flows on Hawai'i Island. Decay rates were greater in the high flow stream than in the low flow one for exotic Spathodea campanulata (0.037 vs. 0.023 day(-1)), but not significantly different for exotic Psidium cattleianum (0.003 vs. 0.003 day(-1)), and native Metrosideros polymorpha (0.005 vs. 0.002 day(-1)). In contrast, the exotic Falcataria moluccana (a nitrogen fixer) decomposed more rapidly in the low flow stream (0.017 day(-1)) than in the high flow stream (0.010 day(-1)). Breakdown rates also varied among species, with S. campanulata > F. moluccana > M. polymorpha > P. cattleianum. Breakdown rates were generally positively correlated to leaf nitrogen content and negatively correlated with leaf structure characteristics (toughness, organic carbon content, percentage lignin). Our findings indicate that stream flow regimes altered by climate change are likely to influence leaf litter decomposition, and S. campanulata and F. moluccana will likely impact organic matter dynamics in Hawaiian and other Pacific Island streams. However, predicted changes depend on the species composition of riparian leaf litter. C1 [Roberts, Megan; Wiegner, Tracy] Univ Hawaii, Dept Marine Sci, 200 West Kawili St, Hilo, HI 96720 USA. [Strauch, Ayron M.] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, 1910 East West Rd,Sherman 101, Honolulu, HI 96822 USA. [Mackenzie, Richard A.] US Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, 60 Nowelo St, Hilo, HI 96720 USA. RP Strauch, AM (reprint author), Univ Hawaii Manoa, Dept Nat Resources & Environm Management, 1910 East West Rd,Sherman 101, Honolulu, HI 96822 USA. EM astrauch@hawaii.edu FU U.S. Forest Service Pacific Southwest Research Station FX This research was funded by the U.S. Forest Service Pacific Southwest Research Station. Additional support was also provided by the Marine Science Department of the University of Hawai'i at Hilo for the analysis of leaf samples. Manuscript accepted 16 October 2015. NR 66 TC 0 Z9 0 U1 13 U2 19 PU UNIV HAWAII PRESS PI HONOLULU PA 2840 KOLOWALU ST, HONOLULU, HI 96822 USA SN 0030-8870 EI 1534-6188 J9 PAC SCI JI Pac. Sci. PD APR PY 2016 VL 70 IS 2 BP 209 EP 222 DI 10.2984/70.2.7 PG 14 WC Marine & Freshwater Biology; Zoology SC Marine & Freshwater Biology; Zoology GA DI6YY UT WOS:000373647400007 ER PT J AU More, G Regensburger, C Gos, ML Pardini, L Verma, SK Ctibor, J Serrano-Martinez, ME Dubey, JP Venturini, MC AF More, Gaston Regensburger, Cristian Laura Gos, M. Pardini, Lais Verma, Shiv K. Ctibor, Juliana Enrique Serrano-Martinez, Marcos Dubey, Jitender P. Cecilia Venturini, M. TI Sarcocystis masoni, n. sp (Apicomplexa: Sarcocystidae), and redescription of Sarcocystis aucheniae from llama (Lama glama), guanaco (Lama guanicoe) and alpaca (Vicugna pacos) SO PARASITOLOGY LA English DT Article DE Sarcocystis spp. South American camelids; PCR; sequencing; electron microscopy; Argentina; Peru ID MOLECULAR-IDENTIFICATION; FINE-STRUCTURE; PARASITES; GERMANY AB There is considerable confusion concerning the species of Sarcocystis in South American camelids (SAC). Several species names have been used; however, proper descriptions are lacking. In the present paper, we redescribe the macroscopic sarcocyst forming Sarcocystis aucheniae and describe and propose a new name, Sarcocystis masoni for the microscopic sarcocyst forming species. Muscles samples were obtained from llamas (Lama glama) and guanacos (Lama guanicoe) from Argentina and from alpacas (Vicugna pacos) and llamas from Peru. Individual sarcocysts were processed by optical and electron microscopy, and molecular studies. Microscopic sarcocysts of S. masoni were up to 800 mu m long and 35-95 mu m wide, the sarcocyst wall was 2.5-3.5 mu m thick, and had conical to cylindrical villar protrusions (vp) with several microtubules. Each vp had 11 or more rows of knob-like projections. Seven 18S rRNA gene sequences obtained from sarcocysts revealed 95-96% identity with other Sarcocystis spp. sequences reported in the GenBank. Sarcocysts of S. aucheniae were macroscopic, up to 1.2 cm long and surrounded by a dense and laminar 50 mu m thick secondary cyst wall. The sarcocyst wall was up to 10 mu m thick, and had branched vp, appearing like cauliflower. Comparison of the 11 sequences obtained from individual macroscopic cysts evidenced a 98-99% of sequence homology with other S. aucheniae sequences. In conclusion, 2 morphologically and molecularly different Sarcocystis species, S. masoni (microscopic cysts) and S. aucheniae (macroscopic cysts), were identified affecting different SAC from Argentina and Peru. C1 [More, Gaston; Laura Gos, M.; Pardini, Lais; Cecilia Venturini, M.] Univ Nacl La Plata, Fac Ciencias Vet, Lab Inmunoparasitol, RA-1900 La Plata, Buenos Aires, Argentina. [More, Gaston; Laura Gos, M.; Pardini, Lais] Consejo Nacl Invest Cient & Tecn CONICET, Buenos Aires, DF, Argentina. [Regensburger, Cristian; Ctibor, Juliana] Univ Nacl Patagonia Austral, Unidad Acad Caleta Olivia, Santa Cruz, Argentina. [Verma, Shiv K.; Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Enrique Serrano-Martinez, Marcos] Univ Peruana Cayetano Heredia, Fac Med Vet & Zootecnia, Lima, Peru. RP More, G (reprint author), Univ Nacl La Plata, Fac Ciencias Vet, Calle 60 & 118, RA-1900 La Plata, Buenos Aires, Argentina. EM gastonmore@fcv.unlp.edu.ar FU Agencia Nacional de Promocion Cientifica y Tecnologica, Argentina [PICT2012-0506]; MINCYT-CONCYTEC (Argentina-Peru) [PE13-05] FX Financial support was partially obtained through Agencia Nacional de Promocion Cientifica y Tecnologica (PICT2012-0506), Argentina (GM); and by a researcher exchange program PE13-05 MINCYT-CONCYTEC (Argentina-Peru, GM and MESM). NR 28 TC 0 Z9 0 U1 2 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD APR PY 2016 VL 143 IS 5 BP 617 EP 626 DI 10.1017/S003118201600007X PG 10 WC Parasitology SC Parasitology GA DI4LK UT WOS:000373471200010 PM 26932444 ER PT J AU Hillis, V Lubell, M Kaplan, J Doll, D Baumgartner, K AF Hillis, Vicken Lubell, Mark Kaplan, Jonathan Doll, David Baumgartner, Kendra TI The Role of Pest Control Advisers in Preventative Management of Grapevine Trunk Diseases SO PHYTOPATHOLOGY LA English DT Article ID LATE BLIGHT MANAGEMENT; EUTYPA DIEBACK; PRUNING WOUNDS; ADOPTION; SUSCEPTIBILITY; CALIFORNIA; INFECTION; LATA; PROTECTION; YIELD AB Vineyards with trunk diseases (Botryosphaeria dieback, Esca, Eutypa dieback, and Phomopsis dieback) can have negative returns in the long run. Minimizing economic impacts depends on effective management, but adopting a preventative practice after infection occurs may not improve yields. Pest control advisers may reduce grower uncertainty about the efficacy of and need for prevention, which often entails future and unobservable benefits. Here, we surveyed advisers in California to examine their influence over grower decision-making, in the context of trunk diseases, which significantly limit grape production and for which curative practices are unavailable. Our online survey revealed adviser awareness of high disease incidence, and reduced yields and vineyard lifespan. Advisers rated both preventative and postinfection practices positively. Despite higher cost estimates given to postinfection practices, advisers did not recommend preventative practices at higher rates. High recommendation rates were instead correlated with high disease incidence for both preventative and postinfection practices. Recommendation rates declined with increasing cost for preventative, but not for postinfection, practices. Our findings suggest that even when advisers acknowledge the risks of trunk diseases, they may not recommend preventative practices before infection occurs. This underscores the importance of clear outreach, emphasizing both the need for prevention and its long-term cost efficacy. C1 [Hillis, Vicken; Lubell, Mark] Univ Calif Davis, Dept Environm Sci & Policy, One Shields Ave, Davis, CA 95616 USA. [Kaplan, Jonathan] Calif State Univ Sacramento, Dept Econ, 6000 J St, Sacramento, CA 95819 USA. [Doll, David] Univ Calif Cooperat Extens, 2145 Wardrobe Ave, Merced, CA 95341 USA. [Baumgartner, Kendra] USDA ARS, Davis, CA 95616 USA. RP Baumgartner, K (reprint author), USDA ARS, Davis, CA 95616 USA. EM Kendra.Baumgartner@ars.usda.gov FU USDA, National Institute of Food and Agriculture's Specialty Crops Research Initiative program [2012-51181-19954] FX Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. We thank the California Association of Pest Control Advisers (CAPCA) for distributing the survey request via email to their members. D. Gates (Ridge Vineyards, Inc.), R. Smith (UCCE Farm Advisor, Sonoma County), C. Rogers (Paramount Farming Company, Lost Hills, CA), B. Higbee (Paramount Farming Company, Lost Hills, CA), L. Bettiga (UCCE Farm Advisor, Monterey County), and M. Hoffman (Lodi Winegrape Commission, Lodi, CA) provided recommendations and contact information for interviews. We also thank the UCCE Viticulture Farm advisors (S. Vasquez [Fresno County, CA], R. Smith [Sonoma County, CA], M. Cooper [Napa County, CA]), and the private PCAs and consultants (C. Rogers (Paramount Farming Company, Lost Hills, CA), Brad Higbee (Paramount Farming Company, Bakersfield, CA), L. Breyer [Breyer's Vineyard IPM Service, Windsor, CA], R. Loguloso [Frank A. Logoluso Farms, Firebaugh, CA], S. Zervas [Silverado Farming, Napa, CA]) who reviewed the survey during its development. We thank the individuals who provided their time to be interviewed as part of this research. We also thank R. Travadon (USDA-ARS, Davis, CA), M. Battany (UCCE Farm Advisor, San Luis Obispo and Santa Barbara counties), and P. Rolshausen (UCCE Specialist, University of California, Riverside) for comments on a version of this manuscript. This research was funded by grant 2012-51181-19954 to K. Baumgartner and M. Lubell from the USDA, National Institute of Food and Agriculture's Specialty Crops Research Initiative program. NR 55 TC 0 Z9 0 U1 7 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 4 BP 339 EP 347 DI 10.1094/PHYTO-10-15-0250-R PG 9 WC Plant Sciences SC Plant Sciences GA DI5FA UT WOS:000373523100003 PM 26645645 ER PT J AU Cheng, P Chen, XM See, DR AF Cheng, P. Chen, X. M. See, D. R. TI Grass Hosts Harbor More Diverse Isolates of Puccinia striiformis Than Cereal Crops SO PHYTOPATHOLOGY LA English DT Article DE microsatellite markers; population structure; yellow rust ID F-SP-TRITICI; WHEAT STRIPE RUST; US PACIFIC-NORTHWEST; UNITED-STATES; F.SP TRITICI; ALTERNATE HOST; YELLOW RUST; MICROSATELLITE LOCI; VIRULENCE VARIATION; SP HORDEI AB Puccinia striifonnis causes stripe rust on cereal crops and many grass species. However, it is not clear whether the stripe rust populations on grasses are able to infect cereal crops and how closely they are related to each other. In this study, 103 isolates collected from wheat, barley, triticale, rye, and grasses in the United States were characterized by virulence tests and simple sequence repeat (SSR) markers. Of 69 pathotypes identified, 41 were virulent on some differentials of wheat only, 10 were virulent on some differentials of barley only, and 18 were virulent on some differentials of both wheat and barley. These pathotypes were clustered into three groups: group one containing isolates from wheat, triticale, rye, and grasses; group two isolates were from barley and grasses; and group three isolates were from grasses and wheat. SSR markers identified 44 multilocus genotypes (MLGs) and clustered them into three major molecular groups (MG) with MLGs in MG3 further classified into three subgroups. Isolates from cereal crops were present in one or more of the major or subgroups, but not all, whereas grass isolates were present in all of the major and subgroups. The results indicate that grasses harbor more diverse isolates of P. striifonnis than the cereals. C1 [Cheng, P.; Chen, X. M.; See, D. R.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X. M.; See, D. R.] USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. RP Chen, XM (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA.; Chen, XM (reprint author), USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. EM xianming@wsu.edu FU USDA, Agricultural Research Service [5348-22000-015-00D]; Washington State University [13C-3061-3925, 13C-3061-5665, PPNS 0651]; Agricultural Research Center, HATCH Project [WNP00663]; Washington State University, Pullman [99164-6430]; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences FX Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. This research was supported by the USDA, Agricultural Research Service (Project 5348-22000-015-00D) and Washington State University (Project 13C-3061-3925; 13C-3061-5665) PPNS 0651, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, HATCH Project WNP00663, Washington State University, Pullman 99164-6430. We thank M. Wang for technical support of the rust DNA extraction; L. Penman and A. Wan for increasing urediniospores and identifying races for some of the stripe rust isolates used in this study; R. A. McIntosh and C. Qualset for their help in stripe rust collection in California; R. A. McIntosh for reviewing an early version of the manuscript and his valuable suggestions; and S. Hulbert, T. Peever, and K. Gill for critical review of the manuscript. NR 59 TC 2 Z9 2 U1 7 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 4 BP 362 EP 371 PG 10 WC Plant Sciences SC Plant Sciences GA DI5FA UT WOS:000373523100006 PM 26667189 ER PT J AU Kolmer, JA Acevedo, MA AF Kolmer, J. A. Acevedo, M. A. TI Genetically Divergent Types of the Wheat Leaf Fungus Puccinia triticina in Ethiopia, a Center of Tetraploid Wheat Diversity SO PHYTOPATHOLOGY LA English DT Article ID F-SP TRITICI; VIRULENCE PHENOTYPES; DURUM-WHEAT; WORLDWIDE COLLECTION; MOLECULAR GENOTYPES; RUST; DIFFERENTIATION; POPULATIONS; RESISTANCE; SPECIALIZATION AB Collections of Puccinia triticina, the wheat leaf rust fungus, were obtained from tetraploid and hexaploid wheat in the central highlands of Ethiopia, and a smaller number from Kenya, from 2011 to 2013, in order to determine the genetic diversity of this wheat pathogen in a center of host diversity. Single-uredinial isolates were derived and tested for virulence phenotype to 20 lines of Thatcher wheat that differ for single leaf rust resistance genes and for molecular genotypes with 10 simple sequence repeat (SSR) primers. Nine virulence phenotypes were described among the 193 isolates tested for virulence. Phenotype BBBQJ, found only in Ethiopia, was predominantly collected from tetraploid wheat. Phenotype EEEEE, also found only in Ethiopia, was exclusively collected from tetraploid wheat and was avirulent to the susceptible hexaploid wheat 'Thatcher'. Phenotypes MBDSS and MCDSS, found in both Ethiopia and Kenya, were predominantly collected from common wheat. Phenotypes CCMSS, CCPSS, and CBMSS were found in Ethiopia from common wheat at low frequency. Phenotypes TCBSS and TCBSQ were found on durum wheat and common wheat in Kenya. Four groups of distinct SSR genotypes were described among the 48 isolates genotyped. Isolates with phenotypes BBBQJ and EEEEE were in two distinct SSR groups, and isolates with phenotypes MBDSS and MCDSS were in a third group. Isolates with CCMSS, CCPSS, CBMSS, TCBSS, and TCBSQ phenotypes were in a fourth SSR genotype group. The diverse host environment of Ethiopia has selected and maintained a genetically divergent population of P. triticina. C1 [Kolmer, J. A.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Acevedo, M. A.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM Jim.Kolmer@ars.usda.gov NR 41 TC 1 Z9 1 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 4 BP 380 EP 385 DI 10.1094/PHYTO-10-15-0247-R PG 6 WC Plant Sciences SC Plant Sciences GA DI5FA UT WOS:000373523100008 PM 26756826 ER PT J AU Janisiewicz, WJ Takeda, F Glenn, DM Camp, MJ Jurick, WM AF Janisiewicz, Wojciech J. Takeda, Fumiomi Glenn, D. Michael Camp, Mary J. Jurick, Wayne M., II TI Dark Period Following UV-C Treatment Enhances Killing of Botrytis cinerea Conidia and Controls Gray Mold of Strawberries SO PHYTOPATHOLOGY LA English DT Article ID POWDERY MILDEW; INDUCED RESISTANCE; ULTRAVIOLET-RADIATION; BIOCONTROL AGENTS; STORAGE ROT; FRUIT ROT; FUNGICIDE APPLICATIONS; POSTHARVEST DISEASES; PRODUCTION SYSTEMS; ERYSIPHE-GRAMINIS AB Strawberries are available throughout the year either from production in the field or from high and low tunnel culture. Diversity of production conditions results in new challenges in controlling diseases before and after harvest. Fungicides have traditionally been used to control these diseases; however, their limitations necessitate a search for new approaches. We found that UV-C irradiation of Botrytis cinerea, a major pathogen of strawberry, can effectively kill this fungus if a dark period follows the treatment. The inclusion of a 4-h dark period resulted in almost complete kill of B. cinerea conidia on agar media at a dose of 12.36 J/m(2). The UV-C dose did not cause a reduction in photosynthesis in strawberry leaves or discoloration of sepals, even after exposing plants repeatedly (twice a week) for 7 weeks. Although irradiation of dry conidia of B. cinerea with this dose resulted in some survival, the conidia were not infective and not able to cause decay even when inoculated onto a highly susceptible mature apple fruit. Irradiation of strawberry pollen at 12.36 J/m(2) did not affect pollen germination, tube growth and length in vitro, or germination and tube growth in the style of hand-pollinated emasculated strawberry flowers. No negative effect of the UV-C treatment was observed on fruit yield and quality in high tunnel culture. In the fruit and flower petal inoculation tests, the UV-C treatment was highly effective in reducing fruit decay and petal infection. This UV-C treatment with an exposure time of 60 s may be useful in controlling gray mold in tunnel production of strawberries and may also have the potential for use in intensive field and indoor production of other fruits and vegetables providing that a 4-h dark period follows the irradiation. C1 [Janisiewicz, Wojciech J.; Takeda, Fumiomi; Glenn, D. Michael] USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Camp, Mary J.] USDA ARS, Stat Grp, Northeast Area, Beltsville, MD 20705 USA. [Jurick, Wayne M., II] USDA ARS, Henry A Wallace Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. RP Janisiewicz, WJ (reprint author), USDA ARS, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM Wojeiech.Janisiewicz@ars.usda.gov FU USDA [CRIS 8080-22000-009-00D] FX We thank S. Yarrington and B. Nichols for excellent technical assistance in conducting UV-C experiments, S. Wolford for his invaluable skills in making a dependable UV-C irradiation apparatus, and N. Snyder for maintaining strawberry plants. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. This research was supported by USDA CRIS 8080-22000-009-00D. NR 59 TC 2 Z9 2 U1 4 U2 22 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD APR PY 2016 VL 106 IS 4 BP 386 EP 394 DI 10.1094/PHYTO-09-15-0240-R PG 9 WC Plant Sciences SC Plant Sciences GA DI5FA UT WOS:000373523100009 PM 26714103 ER PT J AU Wells-Hansen, LD Polashock, JJ Vorsa, N Lockhart, BEL McManus, PS AF Wells-Hansen, L. D. Polashock, J. J. Vorsa, N. Lockhart, B. E. L. McManus, P. S. TI Identification of Tobacco streak virus in Cranberry and the Association of TSV with Berry Scarring SO PLANT DISEASE LA English DT Article ID PRUNUS NECROTIC RINGSPOT; YELLOW-SPOT-VIRUS; SEED TRANSMISSION; MOSAIC DISEASE; HOST-RANGE; BLUEBERRY; POLLEN; ILARVIRUS; PHYLOGENIES; STRAWBERRY AB Cranberry plants bearing disfigured, scarred fruit were reported by growers in the major cranberry-growing region of central Wisconsin in July 2012. Plants bearing scarred fruit have since been observed in Massachusetts and New Jersey. Three complementary methods provided evidence of Tobacco streak virus (TSV) in symptomatic plants: (i) leaves and scarred berries tested positive for TSV by double-antibody sandwich enzyme-linked immunosorbent assay; (ii) quasi-isometric particles approximately 33 nrn in diameter were extracted from leaves of symptomatic plants and visualized using transmission electron microscopy; and (iii) coat protein gene sequence analysis revealed 94 to 99% nucleotide similarity with reference TSV sequences. In newer cultivars, 99% of uprights with scarred berries tested positive for TSV. In older cultivars, 31% of uprights with scarred berries tested positive for TSV and the remaining 69% of uprights with scarred berries tested positive for Blueberry shock virus. TSV overwintered in cranberry plants, and leaves, pollen, and fruit tested positive for TSV the year following symptom occurrence. Attempts to inoculate cranberry using infected pollen or sap as inoculum failed, but several herbaceous hosts tested TSV positive following mechanical inoculation. Phylogenetic analysis of the coat protein gene of 26 TSV isolates from various cultivars of cranberry in Wisconsin, New Jersey, and Massachusetts revealed diversity. This work provides information that will be useful in understanding the epidemiology of TSV in cranberry and in the development of management strategies. C1 [Wells-Hansen, L. D.; McManus, P. S.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Polashock, J. J.] USDA ARS, GIFVL, Chatsworth, NJ 08019 USA. [Vorsa, N.] Rutgers State Univ, Philip E Marucci Ctr Blueberry & Cranberry Res, Chatsworth, NJ 08019 USA. [Lockhart, B. E. L.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. RP McManus, PS (reprint author), Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. EM psm@plantpath.wisc.edu FU National Institute of Food and Agriculture, United States Department of Agriculture [WIS01653]; Wisconsin Cranberry Board, Inc.; Ocean Spray Cranberries, Inc.; Cranberry Institute; Vaughan-Bascom Professorship of the Department of Plant Pathology, University of Wisconsin-Madison; Senator Robert Caldwell Graduate Fellowship of the College of Agricultural and Life Sciences, University of Wisconsin-Madison FX We thank our cranberry grower-cooperators, Wisconsin crop consultants, and Ocean Spray crop consultants for use of field sites and assistance with sample collection; and A. Cramer, K. Adams, V. Kartanos, A. Charkowski, A. Witherell, T. German, E. Saalau-Rojas, R. Serres, and B. Martin for assistance with sample collection and processing and helpful discussions. This research was funded by the National Institute of Food and Agriculture, United States Department of Agriculture, under ID number WIS01653; Wisconsin Cranberry Board, Inc.; Ocean Spray Cranberries, Inc.; Cranberry Institute; Vaughan-Bascom Professorship of the Department of Plant Pathology, University of Wisconsin-Madison; and the Senator Robert Caldwell Graduate Fellowship of the College of Agricultural and Life Sciences, University of Wisconsin-Madison. NR 50 TC 1 Z9 1 U1 4 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD APR PY 2016 VL 100 IS 4 BP 696 EP 703 DI 10.1094/PDIS-06-15-0710-RE PG 8 WC Plant Sciences SC Plant Sciences GA DI7DB UT WOS:000373658200005 ER PT J AU Twomey, MC Stone, JK Gent, DH AF Twomey, M. C. Stone, J. K. Gent, D. H. TI Black Wilt of Hop (Humulus lupulus) Caused by Diplodia seriata in New York State SO PLANT DISEASE LA English DT News Item C1 [Twomey, M. C.; Stone, J. K.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Twomey, MC (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. NR 4 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD APR PY 2016 VL 100 IS 4 BP 861 EP 861 DI 10.1094/PDIS-10-15-1140-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DI7DB UT WOS:000373658200048 ER PT J AU Patpour, M Hovmoller, MS Shahin, AA Newcomb, M Olivera, P Jin, Y Luster, D Hodson, D Nazari, K Azab, M AF Patpour, M. Hovmoller, M. S. Shahin, A. A. Newcomb, M. Olivera, P. Jin, Y. Luster, D. Hodson, D. Nazari, K. Azab, M. TI First Report of the Ug99 Race Group of Wheat Stem Rust, Puccinia graminis f. sp tritici, in Egypt in 2014 SO PLANT DISEASE LA English DT News Item C1 [Patpour, M.; Hovmoller, M. S.] Aarhus Univ, DK-4200 Slagelse, Denmark. [Shahin, A. A.] Plant Pathol Res Inst, Kafrelsheikh 33717, ARC, Egypt. [Newcomb, M.; Olivera, P.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Jin, Y.] USDA ARS, St Paul, MN USA. [Luster, D.] USDA ARS, Foreign Dis Weed Sci Res Unite, Ft Detrick, MD USA. [Hodson, D.] CIMMYT Ethiopia, Addis Ababa, Ethiopia. [Nazari, K.] ICARDA Turkey, Reg Cereal Rust Res Ctr, Aegean Agr Res Inst, PK 9, Izmir, Turkey. [Azab, M.] Wheat Res Program, Arc, Egypt. RP Patpour, M (reprint author), Aarhus Univ, DK-4200 Slagelse, Denmark. NR 3 TC 6 Z9 6 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD APR PY 2016 VL 100 IS 4 BP 863 EP 863 DI 10.1094/PDIS-08-15-0938-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DI7DB UT WOS:000373658200054 ER PT J AU Insuasti, ML Ochoa, JB Martin, RR Alvarez, RA Quito-Avila, DF AF Insuasti, M. L. Ochoa, J. B. Martin, R. R. Alvarez, R. A. Quito-Avila, D. F. TI First Report of Potato virus V and Peru tomato mosaic virus on Tamarillo (Solanum betaceum) Orchards of Ecuador SO PLANT DISEASE LA English DT News Item ID COMPLEX C1 [Insuasti, M. L.; Ochoa, J. B.] INIAP, Inst Nacl Autonomo Invest Agr, Quito, Ecuador. [Martin, R. R.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Alvarez, R. A.; Quito-Avila, D. F.] CIBE ESPOL, Escuela Super Politecn Litoral, Ctr Invest Biotecnol Ecuador, Quito, Ecuador. [Quito-Avila, D. F.] Escuela Super Politecn Litoral, Fac Ciencias Vida, Km 30-5 Via Perimetral Campus Gustavo Galindo, Guayaquil 09015863, Ecuador. RP Insuasti, ML (reprint author), INIAP, Inst Nacl Autonomo Invest Agr, Quito, Ecuador. NR 5 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD APR PY 2016 VL 100 IS 4 BP 868 EP 869 DI 10.1094/PDIS-09-15-1063-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DI7DB UT WOS:000373658200068 ER PT J AU Lanning, KK Moore, PP Keller, KE Martin, RR AF Lanning, K. K. Moore, P. P. Keller, K. E. Martin, R. R. TI First Report of a Resistance-breaking Strain of Raspberry bushy dwarf virus in Red Raspberry (Rubus idaeus) in North America SO PLANT DISEASE LA English DT News Item ID TIME RT-PCR C1 [Lanning, K. K.; Moore, P. P.] Washington State Univ, Puyallup Res & Extens Ctr, Puyallup, WA 98371 USA. [Keller, K. E.; Martin, R. R.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97331 USA. RP Lanning, KK (reprint author), Washington State Univ, Puyallup Res & Extens Ctr, Puyallup, WA 98371 USA. NR 4 TC 0 Z9 0 U1 3 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD APR PY 2016 VL 100 IS 4 BP 868 EP 868 DI 10.1094/PDIS-09-15-1011-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DI7DB UT WOS:000373658200067 ER PT J AU Carbonari, CA Latorre, DO Gomes, GLGC Velini, ED Owens, DK Pan, ZQ Dayan, FE AF Carbonari, Caio A. Latorre, Debora O. Gomes, Giovanna L. G. C. Velini, Edivaldo D. Owens, Daniel K. Pan, Zhiqiang Dayan, Franck E. TI Resistance to glufosinate is proportional to phosphinothricin acetyltransferase expression and activity in LibertyLink (R) and WideStrike (R) cotton SO PLANTA LA English DT Article DE Ammonia; Glutamate; Photosynthesis; Marker gene; Pat; Bar; Injury; Gossypium hirsutum L; Glufosinate ammonium; Cotton ID TIME PCR EXPERIMENTS; 485 WRF COTTON; GLUTAMINE-SYNTHETASE; AMMONIUM ACCUMULATION; UNTRANSFORMED PLANTS; GOSSYPIUM-HIRSUTUM; TRANSGENIC PLANTS; PALMER AMARANTH; REFERENCE GENES; HERBICIDE AB Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower expression and commensurate reduction in PAT activity is a key factor in the low level of injury observed in the WideStrike (A (R)) cotton and relatively high level of resistance observed in LibertyLink (A (R)) cotton. LibertyLink(A (R)) cotton cultivars are engineered for glufosinate resistance by overexpressing the bar gene that encodes phosphinothricin acetyltransferase (PAT), whereas the insect-resistant WideStrike(A (R)) cultivars were obtained using the similar pat gene as a selectable marker. The latter cultivars carry some level of resistance to glufosinate which enticed certain farmers to select this herbicide for weed control with WideStrike(A (R)) cotton. The potency of glufosinate on conventional FM 993, insect-resistant FM 975WS, and glufosinate-resistant IMACD 6001LL cotton cultivars was evaluated and contrasted to the relative levels of PAT expression and activity. Conventional cotton was sensitive to glufosinate. The single copy of the pat gene present in the insect-resistant cultivar resulted in very low RNA expression of the gene and undetectable PAT activity in in vitro assays. Nonetheless, the presence of this gene provided a good level of resistance to glufosinate in terms of visual injury and effect on photosynthetic electron transport. The injury is proportional to the amount of ammonia accumulation. The strong promoter associated with bar expression in the glufosinate-resistant cultivar led to high RNA expression levels and PAT activity which protected this cultivar from glufosinate injury. While the insect-resistant cultivar demonstrated a good level of resistance to glufosinate, its safety margin is lower than that of the glufosinate-resistant cultivar. Therefore, farmers should be extremely careful in using glufosinate on cultivars not expressly designed and commercialized as resistant to this herbicide. C1 [Carbonari, Caio A.; Latorre, Debora O.; Gomes, Giovanna L. G. C.; Velini, Edivaldo D.] Sao Paulo State Univ, Fac Agron Sci, Botucatu, SP, Brazil. [Owens, Daniel K.; Pan, Zhiqiang; Dayan, Franck E.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Dayan, Franck E.] Colorado State Univ, Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. RP Dayan, FE (reprint author), USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA.; Dayan, FE (reprint author), Colorado State Univ, Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM fdayan@olemiss.edu FU Sao Paulo Research Foundation (FAPESP) FX We thank Susan B. Watson and Marilyn Ruscoe for their excellent technical support. Caio Carbonari is also grateful for the financial support from to Sao Paulo Research Foundation (FAPESP) during his work at the USDA-ARS Natural Product Utilization Research Unit. NR 46 TC 2 Z9 3 U1 7 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD APR PY 2016 VL 243 IS 4 BP 925 EP 933 DI 10.1007/s00425-015-2457-3 PG 9 WC Plant Sciences SC Plant Sciences GA DI2EZ UT WOS:000373309800005 PM 26733464 ER PT J AU Bannantine, JP Lingle, CK Adam, PR Ramyar, KX McWhorter, WJ Stabel, JR Picking, WD Geisbrecht, BV AF Bannantine, John P. Lingle, Cari K. Adam, Philip R. Ramyar, Kasra X. McWhorter, William J. Stabel, Judith R. Picking, William D. Geisbrecht, Brian V. TI NlpC/P60 domain-containing proteins of Mycobacterium avium subspecies paratuberculosis that differentially bind and hydrolyze peptidoglycan SO PROTEIN SCIENCE LA English DT Article DE Mycobacterium; Johne's disease; crystal structure; peptidoglycan; proteins; antigens; paratuberculosis ID DISEASE-CONTROL PROGRAM; JOHNES-DISEASE; STRUCTURAL FEATURES; DATA-COLLECTION; DAIRY HERDS; CYSTEINE; INACTIVATION; SUPERFAMILY; ENVIRONMENT; VACCINATION AB A subset of proteins containing NlpC/P60 domains are bacterial peptidoglycan hydrolases that cleave noncanonical peptide linkages and contribute to cell wall remodeling as well as cell separation during late stages of division. Some of these proteins have been shown to cleave peptidoglycan in Mycobacterium tuberculosis and play a role in Mycobacterium marinum virulence of zebra fish; however, there are still significant knowledge gaps concerning the molecular function of these proteins in Mycobacterium avium subspecies paratuberculosis (MAP). The MAP genome sequence encodes five NlpC/P60 domain-containing proteins. We describe atomic resolution crystal structures of two such MAP proteins, MAP_1272c and MAP_1204. These crystal structures, combined with functional assays to measure peptidoglycan cleavage activity, led to the observation that MAP_1272c does not have a functional catalytic core for peptidoglycan hydrolysis. Furthermore, the structure and sequence of MAP_1272c demonstrate that the catalytic residues normally required for hydrolysis are absent, and the protein does not bind peptidoglycan as efficiently as MAP_1204. While the NlpC/P60 catalytic triad is present in MAP_1204, changing the catalytic cysteine-155 residue to a serine significantly diminished catalytic activity, but did not affect binding to peptidoglycan. Collectively, these findings suggest a broader functional repertoire for NlpC/P60 domain-containing proteins than simply hydrolases. PDB Code(s): ; C1 [Bannantine, John P.; Stabel, Judith R.] USDA ARS, Natl Anim Dis Ctr, 1900 Dayton Ave, Ames, IA 50010 USA. [Lingle, Cari K.; Ramyar, Kasra X.; McWhorter, William J.; Geisbrecht, Brian V.] Univ Missouri, Sch Biol Sci, Kansas City, MO 64110 USA. [Adam, Philip R.; Picking, William D.] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA. [Ramyar, Kasra X.] Kansas State Univ, Dept Biochem & Mol Biophy, Manhattan, KS 66506 USA. [Picking, William D.] Univ Kansas, Sch Pharm, Dept Pharmaceut Chem, Lawrence, KS 66045 USA. RP Bannantine, JP (reprint author), USDA ARS, Natl Anim Dis Ctr, 1900 Dayton Ave, Ames, IA 50010 USA.; Geisbrecht, BV (reprint author), Kansas State Univ, Dept Biochem & Mol Biophy, Manhattan, KS 66506 USA. EM john.bannantine@ars.usda.gov; geisbrechtb@ksu.edu OI Bannantine, John/0000-0002-5692-7898 FU Missouri Life Sciences Research Board [13238]; USDA-Agricultural Research Service FX Grant sponsor: Missouri Life Sciences Research Board; Grant number: MO-LSRB #13238; Grant sponsor: USDA-Agricultural Research Service. NR 46 TC 2 Z9 2 U1 0 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0961-8368 EI 1469-896X J9 PROTEIN SCI JI Protein Sci. PD APR PY 2016 VL 25 IS 4 BP 840 EP 851 DI 10.1002/pro.2884 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DI8DT UT WOS:000373732100007 PM 26799947 ER PT J AU Hicks, CC Levine, A Agrawal, A Basurto, X Breslow, SJ Carothers, C Charnley, S Coulthard, S Dolsak, N Donatuto, J Garcia-Quijano, C Mascia, MB Norman, K Poe, MR Satterfield, T Martin, KS Levin, PS AF Hicks, Christina C. Levine, Arielle Agrawal, Arun Basurto, Xavier Breslow, Sara J. Carothers, Courtney Charnley, Susan Coulthard, Sarah Dolsak, Nives Donatuto, Jamie Garcia-Quijano, Carlos Mascia, Michael B. Norman, Karma Poe, Melissa R. Satterfield, Terre Martin, Kevin St. Levin, Phillip S. TI Engage key social concepts for sustainability SO SCIENCE LA English DT Editorial Material C1 [Hicks, Christina C.] Stanford Univ, Ctr Ocean Solut, Stanford, CA 94305 USA. [Hicks, Christina C.] James Cook Univ, Australian Res Council Ctr Excellence Coral Reef, Sydney, NSW, Australia. [Hicks, Christina C.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YW, England. [Levine, Arielle] San Diego State Univ, Geog, San Diego, CA 92182 USA. [Agrawal, Arun] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. [Basurto, Xavier] Duke Univ, Nicholas Sch Environm, Durham, NC 27706 USA. [Breslow, Sara J.; Norman, Karma; Poe, Melissa R.; Levin, Phillip S.] NOAA, Northwest Fisheries Sci Ctr, Tacoma, WA USA. [Carothers, Courtney] Univ Alaska Fairbanks, Sch Fisheries & Ocean Sci, Fairbanks, AK USA. [Charnley, Susan] US Forest Serv, USDA, Washington, DC USA. [Coulthard, Sarah] Northumbria Univ, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, England. [Dolsak, Nives] Univ Washington, Sch Marine & Environm airs, Seattle, WA 98195 USA. [Donatuto, Jamie] Swinomish Indian Tribal Community, Tacoma, WA USA. [Garcia-Quijano, Carlos] Univ Rhode Isl, Anthropol & Marine Affairs, Kingston, RI 02881 USA. [Mascia, Michael B.] Conservat Int, Moore Ctr Sci, Arlington, VA USA. [Poe, Melissa R.] Univ Washington, Washington Sea Grant, Seattle, WA 98195 USA. [Satterfield, Terre] Univ British Columbia, Inst Resources Environm & Sustainabil, Vancouver, BC V5Z 1M9, Canada. [Martin, Kevin St.] Rutgers State Univ, Dept Geog, Piscataway, NJ 08855 USA. RP Hicks, CC (reprint author), Stanford Univ, Ctr Ocean Solut, Stanford, CA 94305 USA.; Hicks, CC (reprint author), James Cook Univ, Australian Res Council Ctr Excellence Coral Reef, Sydney, NSW, Australia.; Hicks, CC (reprint author), Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YW, England. EM christina.hicks@lancaster.ac.uk OI Hicks, Christina/0000-0002-7399-4603 NR 15 TC 10 Z9 10 U1 13 U2 32 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD APR 1 PY 2016 VL 352 IS 6281 BP 38 EP 40 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH8JP UT WOS:000373039600023 PM 27034361 ER PT J AU Carriere, Y Fabrick, JA Tabashnik, BE AF Carriere, Yves Fabrick, Jeffrey A. Tabashnik, Bruce E. TI Can Pyramids and Seed Mixtures Delay Resistance to Bt Crops? SO TRENDS IN BIOTECHNOLOGY LA English DT Review ID FIELD-EVOLVED RESISTANCE; BACILLUS-THURINGIENSIS TOXINS; WESTERN CORN-ROOTWORM; INSECT RESISTANCE; PINK-BOLLWORM; CROSS-RESISTANCE; TRANSGENIC COTTON; PECTINOPHORA-GOSSYPIELLA; LEPIDOPTERA-NOCTUIDAE; LARVAL MOVEMENT AB The primary strategy for delaying the evolution of pest resistance to transgenic crops that produce insecticidal proteins from Bacillus thuringiensis (Bt) entails refuges of plants that do not produce Bt toxins and thus allow survival of susceptible pests. Recent advances include using refuges together with Bt crop 'pyramids' that make two or more Bt toxins effective against the same pest, and planting seed mixtures yielding random distributions of pyramided Bt and non-Bt corn plants within fields. We conclude that conditions often deviate from those favoring the success of pyramids and seed mixtures, particularly against pests with low inherent susceptibility to Bt toxins. For these problematic pests, promising approaches include using larger refuges and integrating Bt crops with other pest management tactics. C1 [Carriere, Yves; Tabashnik, Bruce E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Fabrick, Jeffrey A.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Carriere, Y (reprint author), Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. EM ycarrier@ag.arizona.edu FU USDA Biotechnology Risk Assessment Grant [2014-33522-22214] FX This study was supported by USDA Biotechnology Risk Assessment Grant Award 2014-33522-22214. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. USDA is an equal opportunity provider and employer. NR 90 TC 10 Z9 12 U1 21 U2 42 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0167-7799 J9 TRENDS BIOTECHNOL JI Trends Biotechnol. PD APR PY 2016 VL 34 IS 4 BP 291 EP 302 DI 10.1016/j.tibtech.2015.12.011 PG 12 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DI7BZ UT WOS:000373655400005 PM 26774592 ER PT J AU Shen, T Jiang, ZL Li, CJ Hu, XC Li, QW AF Shen, Tao Jiang, Zhong-Liang Li, Cong-Jun Hu, Xiao-Chen Li, Qing-Wang TI Effect of alpha-lipoic acid on boar spermatozoa quality during freezing-thawing SO ZYGOTE LA English DT Article DE Alpha-lipoic acid; Antioxidants; Artificial insemination; Boar spermatozoa; Cryopreservation ID SPERM QUALITY; MITOCHONDRIAL-FUNCTION; FATTY-ACIDS; ANTIOXIDANT; RATS; CRYOPRESERVATION; CELLS; SEMEN; POLYSACCHARIDE; PROTECTION AB Alpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing-thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen-thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing-thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml. C1 [Shen, Tao; Jiang, Zhong-Liang; Hu, Xiao-Chen; Li, Qing-Wang] Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. [Li, Cong-Jun] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Li, QW (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China. EM liqingwangysu@aliyun.com FU 12th Five-year Plan Rural Areas State Science and Technology Support Projects [2011BAD19B04-3]; Science and Technology Innovation Project of Shaanxi Province [2011KTCL02-11] FX This research was supported by the 12th Five-year Plan Rural Areas State Science and Technology Support Projects (2011BAD19B04-3) and Science and Technology Innovation Project of Shaanxi Province (2011KTCL02-11). NR 36 TC 0 Z9 0 U1 6 U2 8 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0967-1994 EI 1469-8730 J9 ZYGOTE JI Zygote PD APR PY 2016 VL 24 IS 2 BP 259 EP 265 DI 10.1017/S0967199415000155 PG 7 WC Cell Biology; Developmental Biology; Reproductive Biology SC Cell Biology; Developmental Biology; Reproductive Biology GA DI7ZE UT WOS:000373719700011 PM 26099848 ER PT J AU Rivas, DA McDonald, DJ Rice, NP Haran, PH Dolnikowski, GG Fielding, RA AF Rivas, Donato A. McDonald, Devin J. Rice, Nicholas P. Haran, Prashanth H. Dolnikowski, Gregory G. Fielding, Roger A. TI Diminished anabolic signaling response to insulin induced by intramuscular lipid accumulation is associated with inflammation in aging but not obesity SO AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY LA English DT Article DE aging; insulin; inflammation; sarcopenia; obesity; intramuscular lipids; ceramide; diacylglycerol ID RAT SKELETAL-MUSCLE; KAPPA-B; PROTEIN-SYNTHESIS; FATTY-ACIDS; SARCOPENIC OBESITY; GROWTH-FACTOR; RESISTANCE; CERAMIDE; EXERCISE; MECHANISMS AB The loss of skeletal muscle mass is observed in many pathophysiological conditions, including aging and obesity. The loss of muscle mass and function with aging is defined as sarcopenia and is characterized by a mismatch between skeletal muscle protein synthesis and breakdown. Characteristic metabolic features of both aging and obesity are increases in intramyocellular lipid (IMCL) content in muscle. IMCL accumulation may play a mechanistic role in the development of anabolic resistance and the progression of muscle atrophy in aging and obesity. In the present study, aged and high-fat fed mice were used to determine mechanisms leading to muscle loss. We hypothesized the accumulation of bioactive lipids in skeletal muscle, such as ceramide or diacylglycerols, leads to insulin resistance with aging and obesity and the inability to activate protein synthesis, contributing to skeletal muscle loss. We report a positive association between bioactive lipid accumulation and the loss of lean mass and muscle strength. Obese and aged animals had significantly higher storage of ceramide and diacylglycerol compared with young. Furthermore, there was an attenuated insulin response in components of the mTOR anabolic signaling pathway. We also observed differential increases in the expression of inflammatory cytokines and the phosphorylation of I kappa B alpha with aging and obesity. These data challenge the accepted role of increased inflammation in obesity-induced insulin resistance in skeletal muscle. Furthermore, we have now established I kappa B alpha with a novel function in aging-associated muscle loss that may be independent of its previously understood role as an NF-kappa B inhibitor. C1 [Rivas, Donato A.; McDonald, Devin J.; Rice, Nicholas P.; Haran, Prashanth H.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Dolnikowski, Gregory G.] Tufts Univ, Mass Spectrometry Unit, Boston, MA 02111 USA. [Dolnikowski, Gregory G.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Rivas, DA (reprint author), Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. EM donato.rivas@tufts.edu OI Rivas, Donato/0000-0002-4500-6233 FU U.S. Department of Agriculture [58-1950-0014]; Boston Claude D. Pepper Center Older American Independence Centers [1P30AG031679] FX This material is based on the work supported by the U.S. Department of Agriculture, under agreement 58-1950-0014. The study was also supported by the Boston Claude D. Pepper Center Older American Independence Centers (Grant 1P30AG031679). NR 53 TC 1 Z9 1 U1 2 U2 5 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0363-6119 EI 1522-1490 J9 AM J PHYSIOL-REG I JI Am. J. Physiol.-Regul. Integr. Comp. Physiol. PD APR 1 PY 2016 VL 310 IS 7 BP R561 EP R569 DI 10.1152/ajpregu.00198.2015 PG 9 WC Physiology SC Physiology GA DI1PU UT WOS:000373268800003 PM 26764052 ER PT J AU Tsunoda, F Asztalos, IB Horvath, KV Steiner, G Schaefer, EJ Asztalos, BF AF Tsunoda, Fumiyoshi Asztalos, Ivor B. Horvath, Katalin V. Steiner, George Schaefer, Ernst J. Asztalos, Bela F. TI Fenofibrate, HDL, and cardiovascular disease in Type-2 diabetes: The DAIS trial SO ATHEROSCLEROSIS LA English DT Article DE Fenofibrate; Lipoproteins; sdLDL-C; HDL particles; CVD risk ID CORONARY-HEART-DISEASE; DENSITY-LIPOPROTEIN-CHOLESTEROL; INTERVENTION TRIAL; SUBPOPULATION PROFILE; ATHEROSCLEROSIS INTERVENTION; GEMFIBROZIL TREATMENT; PLASMA-LIPOPROTEINS; METABOLIC SYNDROME; ARTERY-DISEASE; VA-HIT AB Background: There are conflicting reports on the role of fibrates in CVD-risk. Several studies indicate beneficial effects of fibrates on CVD risk in type-2 diabetic patients. We tested how fenofibrate changes lipoprotein subfractions and glucose homeostasis in type-2 diabetic patients. Study design: Selected markers of lipid and glucose homeostasis and inflammation were measured in 204 diabetic patients who participated in the Diabetes Atherosclerosis Intervention Study (DAIS) and were randomly assigned to 200 mg fenofibrate or placebo. Percent changes from baseline until a minimum of 3 years (average 39.6 months) on therapy (end of study) were calculated for all study parameters. Results: The concentrations of total LDL-C and small dense LDL-C (sdLDL-C) did not change on fenofibrate compared to placebo. Compared to placebo, fenofibrate significantly decreased concentrations of triglyceride and remnant-like particle cholesterol (RLP-C) and activity of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)), while significantly increased concentrations of HDL-C. In contrast to other lipid-modifying drugs (e.g. statins) which increase HDL-C by increasing large (alpha-1) HDL particles, fenofibrate increased HDL-C by increasing the smaller, less antiatherogenic HDL-C particles, alpha-3 and alpha-4. Furthermore, despite lowering TG levels by 20%, fenofibrate failed to decrease pre-beta 1 levels. On fenofibrate, glycated serum-protein levels increased moderately, while insulin and adiponectin levels did not change. Conclusion: On fenofibrate, lipid homeostasis improved and Lp-PLA2 activity decreased while there was no improvement in glucose homeostasis. Despite increasing HDL-C and decreasing triglyceride levels, fenofibrate failed to improve the antiatherogenic properties of the HDL subpopulation profile. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Tsunoda, Fumiyoshi; Horvath, Katalin V.; Schaefer, Ernst J.; Asztalos, Bela F.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Asztalos, Ivor B.] Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. [Steiner, George] Univ Hlth Network, Div Endocrinol, Toronto, ON, Canada. [Steiner, George] Univ Toronto, Toronto, ON M5S 1A1, Canada. RP Asztalos, BF (reprint author), Tufts Univ, Cardiovasc Nutr Lab, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM bela.asztalos@tufts.edu FU ABBOTT Laboratories; NIH [HL117933] FX This work was supported by grants from ABBOTT Laboratories and the NIH (HL117933) PI: Asztalos. We thank DiaDexus for measuring LpPLA2 in DAIS samples at no cost. NR 25 TC 0 Z9 1 U1 1 U2 1 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD APR PY 2016 VL 247 BP 35 EP 39 DI 10.1016/j.atherosclerosis.2016.01.028 PG 5 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA DH3XA UT WOS:000372718900005 PM 26854974 ER PT J AU Liebhold, AM Yamanaka, T Roques, A Augustin, S Chown, SL Brockerhoff, EG Pysek, P AF Liebhold, Andrew M. Yamanaka, Takehiko Roques, Alain Augustin, Sylvie Chown, Steven L. Brockerhoff, Eckehard G. Pysek, Petr TI Global compositional variation among native and non-native regional insect assemblages emphasizes the importance of pathways SO BIOLOGICAL INVASIONS LA English DT Article DE Biological invasion; Establishment; Fauna; Island; Introduction pathway; Insect order; Multivariate analysis ID BIOLOGICAL INVASIONS; LATITUDINAL GRADIENT; GALAPAGOS-ISLANDS; PLANT INVASIONS; UNITED-STATES; IMPACTS; ESTABLISHMENT; CONSERVATION; POPULATION; MANAGEMENT AB Insects are among the world's most ecologically and economically important invasive species. Here we assemble inventories of native and non-native species from 20 world regions and contrast relative numbers among these species assemblages. Multivariate ordination indicates that the distribution of species among insect orders is completely different between native and non-native assemblages. Some orders, such as the Psocoptera, Dictyoptera, Siphonaptera, Thysanoptera, and Hemiptera, are always over-represented in the non-native compared to native assemblages. Other orders, such as the Plecoptera, Trichoptera, Ephemeroptera, Odonata, Mecoptera and Microcoryphila, are consistently under-represented in non-native assemblages. These patterns most likely arise both as a result of variation among taxa in their association with invasion pathways responsible for transporting species among world regions, as well as variation in life-history traits that affect establishment potential. However, our results indicate that species compositions associated with invasiveness are fundamentally different from compositions related to insularity, indicating that colonization of islands selects for a different group of insect taxa than does selection for successful invaders. Native and non-native assemblage compositions were also related, to a lesser extent, to latitude of the region sampled. Together, these results illustrate the dominant role of invasion pathways in shaping the composition of non-native insect assemblages. They also emphasize the difference between natural background colonization of islands and anthropogenic colonization events, and imply that biological invasions are not a simple subset of a long-standing ecological process. C1 [Liebhold, Andrew M.] US Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. [Yamanaka, Takehiko] Natl Inst Agroenvironm Sci, Nat Resources Inventory Ctr, Kannon Dai 3-1-3, Tsukuba, Ibaraki 305, Japan. [Roques, Alain; Augustin, Sylvie] INRA, UR0633, Zool Forestiere, CS 40001, F-45075 Orleans, France. [Chown, Steven L.] Monash Univ, Sch Biol Sci, Melbourne, Vic 3800, Australia. [Brockerhoff, Eckehard G.] Scion, POB 29237, Christchurch 8440, New Zealand. [Pysek, Petr] Acad Sci Czech Republic, Inst Bot, CS-25243 Pruhonice, Czech Republic. [Pysek, Petr] Charles Univ Prague, Dept Ecol, Fac Sci, Vinicna 7, CR-12844 Prague 2, Czech Republic. [Pysek, Petr] Univ Stellenbosch, Dept Bot & Zool, Ctr Invas Biol, ZA-7602 Matieland, South Africa. RP Liebhold, AM (reprint author), US Forest Serv, No Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. EM aliebhold@fs.fed.us; apple@affrc.go.jp; alain.roques@orleans.inra.fr; sylvie.augustin@orleans.inra.fr; steven.chown@monash.edu; Eckehard.Brockerhoff@scionresearch.com; pysek@ibot.cas.cz RI Pysek, Petr/B-1957-2012; OI Chown, Steven/0000-0001-6069-5105 FU DST-NRF Centre of Excellence for Invasion Biology at Stellenbosch University, South Africa; HortGro; National Research Foundation of South Africa; Stellenbosch University; SubTrop; European COST project Alien Challenge [TD1209]; European COST project Global Warning [TD1401]; Czech Academy of Sciences [RVO 67985939]; Praemium Academiae award from The Czech Academy of Sciences; Australian Antarctic Science Program [4307]; MBIE FX The November 2014 workshop on "Drivers, impacts, mechanisms and adaptation in insect invasions" was hosted and co-funded by the DST-NRF Centre of Excellence for Invasion Biology at Stellenbosch University, South Africa. Additional financial support was provided by HortGro, the National Research Foundation of South Africa, Stellenbosch University, and SubTrop. The reviewers are thanked for their helpful comments. AR and SA were supported by the European COST projects Alien Challenge (TD1209) and Global Warning (TD1401). PP was supported by long-term research development project RVO 67985939 (The Czech Academy of Sciences) and by a Praemium Academiae award from The Czech Academy of Sciences. SLC was supported by Australian Antarctic Science Program Project 4307. EGB was supported by MBIE core funding to Scion and the Better Border Biosecurity collaboration (www.b3nz.org). NR 78 TC 2 Z9 2 U1 11 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD APR PY 2016 VL 18 IS 4 BP 893 EP 905 DI 10.1007/s10530-016-1079-4 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DI0ZL UT WOS:000373225700002 ER PT J AU Roques, A Auger-Rozenberg, MA Blackburn, TM Garnas, J Pysek, P Rabitsch, W Richardson, DM Wingfield, MJ Liebhold, AM Duncan, RP AF Roques, Alain Auger-Rozenberg, Marie-Anne Blackburn, Tim M. Garnas, Jeff Pysek, Petr Rabitsch, Wolfgang Richardson, David M. Wingfield, Michael J. Liebhold, Andrew M. Duncan, Richard P. TI Temporal and interspecific variation in rates of spread for insect species invading Europe during the last 200 years SO BIOLOGICAL INVASIONS LA English DT Article DE Biological invasions; Insects; Introductions; Spread; Europe; Cold War ID CYDALIMA-PERSPECTALIS WALKER; BOX TREE MOTH; PLANT INVASIONS; BIOLOGICAL INVASIONS; PYRALOIDEA CRAMBIDAE; ALIEN; INTRODUCTIONS; HABITATS; PATTERNS; RECORD AB Globalization is triggering an increase in the establishment of alien insects in Europe, with several species having substantial ecological and economic impacts. We investigated long-term changes in rates of species spread following establishment. We used the total area of countries invaded by 1171 insect species for which the date of first record in Europe is known, to estimate their current range radius (calculated as [invaded area](0.5)/pi). We estimated initial rates of radial spread and compared them among different groups of insects for all years (1800-2014) and for a subset of more recent decades (1950-2014). Accidentally introduced species spread faster than intentionally introduced species. Considering the whole period 1800-2014, spread patterns also differ between feeding guilds, with decreasing spread rates over residence time in herbivores but not in detritivores or parasitic species. These decreases for herbivorous species appeared mainly in those associated with herbaceous plants and crops rather than woody plants. Initial spread rate was significantly greater for species detected after 1990, roughly 3-4 times higher than for species that arrived earlier. We hypothesize that the political changes in Europe following the collapse of the Iron Curtain in 1989, and the further dismantling of customs checkpoints within an enlarged European Union (EU) have facilitated the faster spread of alien insect species. Also, the number of species first recorded in the Eastern Bloc of the politically-divided Europe before 1989 was lower than for the rest of Europe. A detailed analysis of six recent invaders indicated a dominant role of long-distance translocations related to human activities, especially with the plant trade, in determining rates of spread. C1 [Roques, Alain; Auger-Rozenberg, Marie-Anne] INRA, UR 0633, Zool Forestiere, F-45075 Orleans, France. [Blackburn, Tim M.] UCL, Dept Genet Evolut & Environm, Ctr Biodivers & Environm Res, Gower St, London WC1E 6BT, England. [Garnas, Jeff] Univ Pretoria, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa. [Garnas, Jeff; Wingfield, Michael J.] Univ Pretoria, Forestry & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa. [Pysek, Petr] Acad Sci Czech Republic, Inst Bot, CS-25243 Pruhonice, Czech Republic. [Pysek, Petr] Charles Univ Prague, Fac Sci, Dept Ecol, Vinicna 7, CR-12844 Prague 2, Czech Republic. [Rabitsch, Wolfgang] Environm Agcy Austria, Vienna, Austria. [Richardson, David M.] Univ Stellenbosch, Dept Bot & Zool, Ctr Invas Biol, ZA-7602 Matieland, South Africa. [Liebhold, Andrew M.] US Forest Serv, No Res Stn, Morgantown, WV 26505 USA. [Duncan, Richard P.] Univ Canberra, Inst Appl Ecol, Canberra, ACT 2601, Australia. RP Roques, A (reprint author), INRA, UR 0633, Zool Forestiere, F-45075 Orleans, France. EM alain.roques@orleans.inra.fr RI Richardson, David/A-1495-2008; Duncan, Richard/E-7899-2013; Pysek, Petr/B-1957-2012 OI Richardson, David/0000-0001-9574-8297; Duncan, Richard/0000-0003-2295-449X; FU DST-NRF Centre of Excellence for Invasion in Stellenbosch, South Africa; HortGro; National Research Foundation of South Africa; Stellenbosch University; SubTrop; Czech Academy of Sciences [RVO 67985939]; Praemium Academiae award from The Czech Academy of Sciences; DST-NRF Centre of Excellence for Invasion Biology; National Research Foundation, South Africa [85417]; European COST project Alien Challenge [TD1209]; European COST project Global Warning [TD1401]; regional project INCA FX This paper had its origin at a workshop on "Drivers, impacts, mechanisms and adaptation in insect invasions" hosted and co-funded by the DST-NRF Centre of Excellence for Invasion in Stellenbosch, South Africa, in November 2014. Additional financial support was provided by HortGro, the National Research Foundation of South Africa, Stellenbosch University, and SubTrop. PP was supported by long-term research development project RVO 67985939 (The Czech Academy of Sciences) and by Praemium Academiae award from The Czech Academy of Sciences. DMR acknowledges support from the DST-NRF Centre of Excellence for Invasion Biology and the National Research Foundation, South Africa (Grant 85417). We thank Lionel Roques and Samuel Soubeyrand, INRA Avignon, France for fruitful discussions about the statistical methods to be used in order to better exploit the data. The European COST projects Alien Challenge (TD1209) and Global Warning (TD1401) are also acknowledged for their support as well as the regional project INCA funded the French Region Centre. NR 57 TC 3 Z9 4 U1 8 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD APR PY 2016 VL 18 IS 4 BP 907 EP 920 DI 10.1007/s10530-016-1080-y PG 14 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DI0ZL UT WOS:000373225700003 ER PT J AU Wagner, R Moon, RJ Raman, A AF Wagner, Ryan Moon, Robert J. Raman, Arvind TI Mechanical properties of cellulose nanomaterials studied by contact resonance atomic force microscopy SO CELLULOSE LA English DT Article DE Atomic force microscopy; Cellulose nanomaterials; Cellulose nanocrystals; Cellulose nanofibrils; Contact resonance; Nanomechanics ID ELASTIC-MODULI; WHISKERS; NANOCRYSTALS; CALIBRATION; SIZE AB Quantification of the mechanical properties of cellulose nanomaterials is key to the development of new cellulose nanomaterial based products. Using contact resonance atomic force microscopy we measured and mapped the transverse elastic modulus of three types of cellulosic nanoparticles: tunicate cellulose nanocrystals, wood cellulose nanocrystals, and wood cellulose nanofibrils. These modulus values were calculated with different contact mechanics models exploring the effects of cellulose geometry and thickness on the interpretation of the data. While intra-particle variations in modulus are detected, we did not observe a measureable difference in modulus between the three types of cellulose particles. Improved practices and experimental complications for the characterization of cellulosic nanomaterials with atomic force microscopy are discussed. C1 [Wagner, Ryan; Raman, Arvind] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA. [Wagner, Ryan; Raman, Arvind] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Wagner, Ryan] NIST, Mat Measurement Lab, Boulder, CO 80305 USA. RP Wagner, R (reprint author), NIST, Mat Measurement Lab, Boulder, CO 80305 USA. EM rbwagner187@gmail.com; robertmoon@fs.fed.us; raman@purdue.edu FU Forest Products laboratory (US Forest Service) [12-CR-11111129-076] FX The authors would like to thank the Forest Products laboratory (US Forest Service) Grant No. 12-CR-11111129-076 for funding this research. We are grateful to the Forest Products laboratory for providing the wood CN samples and Professor Christoph Weder (Universite de Fribourg) for providing tunicate CN samples. We are also grateful for Jason Killgore (NIST) for helpful discussions regarding the contact resonance AFM technique. NR 46 TC 0 Z9 0 U1 10 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2016 VL 23 IS 2 BP 1031 EP 1041 DI 10.1007/s10570-016-0883-4 PG 11 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DH9SO UT WOS:000373136400002 ER PT J AU Nam, S Kim, HJ Condon, BD Hinchliffe, DJ Chang, SC McCarty, JC Madison, CA AF Nam, Sunghyun Kim, Hee Jin Condon, Brian D. Hinchliffe, Doug J. Chang, SeChin McCarty, Jack C. Madison, Crista A. TI High resistance to thermal decomposition in brown cotton is linked to tannins and sodium content SO CELLULOSE LA English DT Article DE Brown cotton fiber; Inorganic salts; Tannins; Thermogravimetry; Microscale combustion calorimetry; NMR ID NATURALLY COLORED COTTONS; MICROSCALE COMBUSTION CALORIMETRY; NATURAL CONDENSED TANNIN; NATIVE CELLULOSES; FIBERS; PYROLYSIS; WOOD; METAL; PIGMENTATION; BEHAVIOR AB Brown cotton fibers (SA-1 and MC-BL) studied were inferior to a white cotton fiber (Sure-Grow 747) in fiber quality, i.e., a shorter length, fewer twists, and lower crystallinity, but showed superior thermal resistance in thermogravimetric, differential thermogravimetric, and microscale combustion calorimetric (MCC) analyses. Brown cotton fibers yielded 11-23 % smaller total heat release and 20-40 % greater char. Washing fibers in water and a 1 % NaOH solution showed that rich natural inorganic components and the condensed tannins present in brown cotton are responsible for the unusual thermal property. The loss of inorganics from white cotton during a water wash increased the thermal decomposition temperature of cellulose, resulting in no char yield. However, the stronger binding of metal ions for brown cotton as well as its dominant adsorption of sodium ions after a 1 % NaOH wash facilitated the low-temperature thermal-reaction route; the sodium content showed a significant negative correlation with the heat release capacity of the fiber. Condensed tannins greatly enhanced the adsorption of sodium ions to the fiber and exhibited inherent thermal stability. The limiting oxygen indices (LOI) calculated from the MCC parameters indicated the slower burning characteristic of brown cotton, and its LOI was further increased upon adsorption of sodium ions. C1 [Nam, Sunghyun; Condon, Brian D.; Hinchliffe, Doug J.; Chang, SeChin; Madison, Crista A.] ARS, Cotton Chem & Utilizat Res Unit, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Kim, Hee Jin] ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [McCarty, Jack C.] ARS, Genet & Sustainable Agr Res Unit, USDA, New Orleans, MS 39762 USA. RP Condon, BD (reprint author), ARS, Cotton Chem & Utilizat Res Unit, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM brian.condon@ars.usda.gov NR 50 TC 1 Z9 1 U1 7 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2016 VL 23 IS 2 BP 1137 EP 1152 DI 10.1007/s10570-016-0871-8 PG 16 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DH9SO UT WOS:000373136400011 ER PT J AU Tonoli, GHD Holtman, KM Glenn, G Fonseca, AS Wood, D Williams, T Sa, VA Torres, L Klamczynski, A Orts, WJ AF Tonoli, G. H. D. Holtman, K. M. Glenn, G. Fonseca, A. S. Wood, D. Williams, T. Sa, V. A. Torres, L. Klamczynski, A. Orts, W. J. TI Properties of cellulose micro/nanofibers obtained from eucalyptus pulp fiber treated with anaerobic digestate and high shear mixing SO CELLULOSE LA English DT Article DE Cellulose fiber; Plant cell wall; Lignocellulosic fiber; Nanofiber; Microfiber; Nanocrystalline cellulose; Thermal properties ID DEGRADING MICROBIAL COMMUNITY; SP-NOV.; MICROFIBRILLATED CELLULOSE; CRYSTALLINE CELLULOSE; ENZYMATIC-HYDROLYSIS; WASTE-WATER; PART 1; BACTEROIDES; WOOD; NANOCOMPOSITES AB High production costs remain the single greatest factor limiting wider use of cellulose micro/nanofibers by industry. The objective of the present study was to investigate the potential of using a low-cost bacteria-rich digestate (liquid anaerobic digestate-AD-supernatant) on milled eucalyptus fiber followed by high-shear mixing to obtain cellulose micro/nanofibers. The morphology, crystallinity, and thermal stability of micro/nanofibers obtained by this process were studied. The bacteria population in the AD-supernatant was comprised mostly of Bacteroides graminisolvens and Parabacteroides chartae. The digestate treatment partially removed amorphous components of the pulp fiber thereby decreasing micro/nanofiber diameters and enhancing the crystalline content. The treatment also increased the size of the crystalline cellulose. The morphology and crystallinity results demonstrate the effectiveness of digestate treatments coupled with high-shear mixing as a procedure for the production of micro/nanofibers. C1 [Tonoli, G. H. D.; Fonseca, A. S.; Sa, V. A.] Univ Fed Lavras, Dept Forest Sci, CP 3037, BR-37200000 Lavras, MG, Brazil. [Tonoli, G. H. D.; Holtman, K. M.; Glenn, G.; Wood, D.; Williams, T.; Torres, L.; Klamczynski, A.; Orts, W. J.] USDA ARS, WRRC, Bioprod Res Unit, Albany, CA 94710 USA. RP Tonoli, GHD (reprint author), Univ Fed Lavras, Dept Forest Sci, CP 3037, BR-37200000 Lavras, MG, Brazil. EM gustavotonoli@yahoo.com.br FU CAPES [BEX 14459/13-2]; CNPq [309796/2012-1, 484082/2013-2, 506506/2013-4]; FAPEMIG, Brazil; United States Department of Agriculture (USDA) FX Authors thank the financial support granted by CAPES (Process No. BEX 14459/13-2), CNPq (Process No. 309796/2012-1; 484082/2013-2; 506506/2013-4), FAPEMIG, Brazil; and United States Department of Agriculture (USDA). Thanks also to Rede Brasileira de Compositos e Nanocompositos Lignocelulosicos (RELIGAR, Brazil), Graduate Program in Biomaterials Engineering (UFLA) and Graduate Program in Wood Science and Technology (UFLA), Brazil. NR 92 TC 0 Z9 0 U1 11 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2016 VL 23 IS 2 BP 1239 EP 1256 DI 10.1007/s10570-016-0890-5 PG 18 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DH9SO UT WOS:000373136400019 ER PT J AU Edwards, JV Fontenot, KR Haldane, D Prevost, NT Condon, BD Grimm, C AF Edwards, J. Vincent Fontenot, Krystal R. Haldane, David Prevost, Nicolette T. Condon, Brian D. Grimm, Casey TI Human neutrophil elastase peptide sensors conjugated to cellulosic and nanocellulosic materials: part I, synthesis and characterization of fluorescent analogs SO CELLULOSE LA English DT Article DE Peptides; Wood cellulose nanocrystals; Wood nanofilms; Human neutrophil elastase ID SEGAL CRYSTALLINITY INDEX; SURFACE; NANOCRYSTALS; BIOSENSORS; NANOPAPER; SPECTROSCOPY; DIAGNOSTICS; WHISKERS; SYSTEMS; FIBERS AB Here we describe the synthesis and characterization of peptide conjugated cellulose and nanocellulose materials as sensors for fluorescent detection of human neutrophil elastase (HNE). The cellulose sensor surfaces selected are filter paper (FP) and print cloth (PC) fabric, which are composed of processed cotton fibers. The nanocellulose based sensors have transducer surfaces comprised of cellulose nanocrystals (wCNC) and microfibrillated cellulose (MFC) derived from wood that are fabricated as wood nanocellulose composites (wNCCs) consisting of blended quantities of nanocrystalline and MFC at 66/33 and 50/50 ratios. These ratios were selected to examine the effect of significantly different CNC loadings had on surface area and peptide uptake. The fluorescent peptide HNE substrate, n-succinyl-Ala-Pro-Ala-4-amido-7-methylcoumarin (Pep) was attached to both cellulosic and nanocellulosic matrices and conjugated peptide analogs were confirmed by mass spectrometry (MS) and infrared (IR). The nanocellulose biosensors wCNC-Pep (3c) and wNCC-Pep (4c, 66/33 and 5c, 50/50) have higher levels of peptide incorporation than the cellulosic biosensors FP-Pep (1c) and PC-Pep (2c). The range of incorporation for the cellulosic sensors is 7-25 mu g/mg and for the nanocellulose sensors 30-80 mu g/mg. The degree of substitution of peptide was found to be in the order of approximate number of peptides per 200 anhydroglucose residues, 1 in PC-Pep (2c), 2 in FP-Pep (1c), 4 in wNCC-Pep (5c, 50/50), 6 in wNCC-Pep (4c, 66/33), and 12 in wCNC-Pep (3c). The specific surface areas of the sensors ranged from 0.016 to 261 m(2) g(-1) and correlated with degree of substitution of peptide on the cellulosic and nanocellulosic surfaces. Of the cellulose and nanocellulose biosensors, the wCNC-Pep (3c) has the highest level of peptide incorporation and the highest specific surface area, which makes it the preferred sensor matrix for human neutrophil elastase. C1 [Edwards, J. Vincent; Fontenot, Krystal R.; Prevost, Nicolette T.; Condon, Brian D.; Grimm, Casey] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Haldane, David] Innovatech Engn, Tallahassee, FL 32317 USA. RP Edwards, JV (reprint author), USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM Vince.Edwards@ars.usda.gov NR 53 TC 1 Z9 1 U1 6 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2016 VL 23 IS 2 BP 1283 EP 1295 DI 10.1007/s10570-016-0869-2 PG 13 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DH9SO UT WOS:000373136400022 ER PT J AU Fontenot, KR Edwards, JV Haldane, D Graves, E Citron, MS Prevost, NT French, AD Condon, BD AF Fontenot, Krystal R. Edwards, J. Vincent Haldane, David Graves, Elena Citron, Michael Santiago Prevost, Nicolette T. French, Alfred D. Condon, Brian D. TI Human neutrophil elastase detection with fluorescent peptide sensors conjugated to cellulosic and nanocellulosic materials: part II, structure/function analysis SO CELLULOSE LA English DT Article DE Peptides; Wood cellulose nanocrystals; Wood nanocellulose composites; Human neutrophil elastase; Biosensors ID THIN-FILMS; NANOCRYSTALS; BIOSENSORS; NANOPAPER AB Human neutrophil elastase (HNE) is one of a number of proteases that is receiving increased attention as a marker for inflammatory diseases and sensor-based point of care diagnostics. Integral to sensor-based detection is the transducer surface, which is the platform of the sensor's signal transmittance. Here we describe the bioactivity and related transducer surface properties of cellulose and nanocellulose matrices as peptide-cellulose fluorescent sensors. Detection sensitivity of the sensor signals for HNE levels typically found in chronic wounds is characterized. The fluorescent elastase peptide substrate, Succinamidyl-Ala-Ala-Pro-Val-amidylcoumadin (Pep) was employed in both cellulose and nanocellulose transducer surfaces evaluated for biosensor sensitivity to HNE. The cellulose transducers selected are filter paper (FP) and print cloth (PC) fabric and are comprised of processed cotton fibers. The nanocellulose transducers are the wood cellulose nanocrystals (wCNC) and the wood nanocellulose composites (wNCC). The wNCCs consist of blended quantities of nanocrystalline and microfibrillated cellulose at 66/33 and 50/50, and are characterized as thin films. The biosensor activity was in the order of wCNC-Pep > FP-Pep = NCC-Pep (50/50) > NCC-Pep (66/33) > PC-Pep. Sensor sensitivity correlated with specific surface area. A depiction of peptide substitution on nanocellulosic and cellulosic surfaces is rendered through peptide-cellulose crystallite models derived from X-ray diffraction analysis of the material, and the models discussed in light of biosensor structure activity relationships. In addition, the overall morphology, pore size and porosity of the materials are discussed for their suitability as protease sensors. C1 [Fontenot, Krystal R.; Edwards, J. Vincent; Graves, Elena; Citron, Michael Santiago; Prevost, Nicolette T.; French, Alfred D.; Condon, Brian D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Haldane, David] Innovatech Engn, Tallahassee, FL 32317 USA. RP Edwards, JV (reprint author), USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM Vince.Edwards@ars.usda.gov NR 38 TC 2 Z9 2 U1 11 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2016 VL 23 IS 2 BP 1297 EP 1309 DI 10.1007/s10570-016-0873-6 PG 13 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DH9SO UT WOS:000373136400023 ER PT J AU You, FM Song, QJ Jia, GF Cheng, YZ Duguid, S Booker, H Cloutier, S AF You, Frank M. Song, Qijian Jia, Gaofeng Cheng, Yanzhao Duguid, Scott Booker, Helen Cloutier, Sylvie TI Estimation of genetic parameters and their sampling variances for quantitative traits in the type 2 modified augmented design SO CROP JOURNAL LA English DT Article DE Broad-sense heritability; Genetic correlation; Sampling variance; Modified augmented design ID LINUM-USITATISSIMUM L.; FATTY-ACID-COMPOSITION; CORRELATION COEFFICIENT; FIELD-EVALUATION; VARIETY TRIALS; BARLEY LINES; LARGE NUMBER; EARLY STAGE; REPLICATION; COVARIANCE AB The type 2 modified augmented design (MAD2) is an efficient unreplicated experimental design used for evaluating large numbers of lines in plant breeding and for assessing genetic variation in a population. Statistical methods and data adjustment for soil heterogeneity have been previously described for this design. In the absence of replicated test genotypes in MAD2, their total variance cannot be partitioned into genetic and error components as required to estimate heritability and genetic correlation of quantitative traits, the two conventional genetic parameters used for breeding selection. We propose a method of estimating the error variance of unreplicated genotypes that uses replicated controls, and then of estimating the genetic parameters. Using the Delta method, we also derived formulas for estimating the sampling variances of the genetic parameters. Computer simulations indicated that the proposed method for estimating genetic parameters and their sampling variances was feasible and the reliability of the estimates was positively associated with the level of heritability of the trait. A case study of estimating the genetic parameters of three quantitative traits, iodine value, oil content, and linolenic acid content, in a biparental recombinant inbred line population of flax with 243 individuals, was conducted using our statistical models. A joint analysis of data over multiple years and sites was suggested for genetic parameter estimation. A pipeline module using SAS and Perl was developed to facilitate data analysis and appended to the previously developed MAD data analysis pipeline (http://probes.pw.usda.gov/bioinformatics_tools/MADPipeline/index. html). (C) 2016 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [You, Frank M.; Jia, Gaofeng; Cheng, Yanzhao; Duguid, Scott] Agr & Agri Food Canada, Morden Res & Dev Ctr, Morden, MB R6M 1Y5, Canada. [Song, Qijian] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Jia, Gaofeng; Booker, Helen] Univ Saskatchewan, Crop Dev Ctr, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada. [Cloutier, Sylvie] Agr & Agri Food Canada, Ottawa Res & Dev Ctr, Ottawa, ON K1A 0C6, Canada. RP You, FM (reprint author), Agr & Agri Food Canada, Morden Res & Dev Ctr, Morden, MB R6M 1Y5, Canada. EM Frank.You@agr.gc.ca NR 38 TC 1 Z9 1 U1 2 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD APR PY 2016 VL 4 IS 2 BP 107 EP 118 DI 10.1016/j.cj.2016.01.003 PG 12 WC Agronomy SC Agriculture GA DH7NN UT WOS:000372980900004 ER PT J AU La Pierre, KJ Blumenthal, DM Brown, CS Klein, JA Smith, MD AF La Pierre, Kimberly J. Blumenthal, Dana M. Brown, Cynthia S. Klein, Julia A. Smith, Melinda D. TI Drivers of Variation in Aboveground Net Primary Productivity and Plant Community Composition Differ Across a Broad Precipitation Gradient SO ECOSYSTEMS LA English DT Article DE dominant species; Great Plains; nutrients; Nutrient Network (NutNet); rare species; precipitation periods ID CENTRAL GRASSLAND REGION; NORTH-AMERICAN; NITROGEN LIMITATION; CLIMATE VARIABILITY; TEMPORAL VARIATION; TALLGRASS PRAIRIE; USE EFFICIENCY; UNITED-STATES; GLOBAL CHANGE; ECOSYSTEM AB Aboveground net primary production (ANPP) is a key integrator of C uptake and energy flow in many terrestrial ecosystems. As such, ecologists have long sought to understand the factors driving variation in this important ecosystem process. Although total annual precipitation has been shown to be a strong predictor of ANPP in grasslands across broad spatial scales, it is often a poor predictor at local scales. Here we examine the amount of variation in ANPP that can be explained by total annual precipitation versus precipitation during specific periods of the year (precipitation periods) and nutrient availability at three sites representing the major grassland types (shortgrass steppe, mixed-grass prairie, and tallgrass prairie) spanning the broad precipitation gradient of the U.S. Central Great Plains. Using observational data, we found that precipitation periods and nutrient availability were much stronger predictors of site-level ANPP than total annual precipitation. However, the specific nutrients and precipitation periods that best predicted ANPP differed among the three sites. These effects were mirrored experimentally at the shortgrass and tallgrass sites, with precipitation and nutrient availability co-limiting ANPP, but not at the mixed-grass site, where nutrient availability determined ANPP exclusive of precipitation effects. Dominant grasses drove the ANPP response to increased nutrient availability at all three sites. However, the relative responses of rare grasses and forbs were greater than those of the dominant grasses to experimental nutrient additions, thus potentially driving species turnover with chronic nutrient additions. This improved understanding of the factors driving variation in ANPP within ecosystems spanning the broad precipitation gradient of the Great Plains will aid predictions of alterations in ANPP under future global change scenarios. C1 [La Pierre, Kimberly J.] Yale Univ, Dept Ecol & Evolutionary Biol, 165 Prospect St, New Haven, CT 06511 USA. [Blumenthal, Dana M.] ARS, Rangeland Resources Res Unit, USDA, 1701 Ctr Ave, Ft Collins, CO 80526 USA. [Brown, Cynthia S.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Campus Delivery 1177, Ft Collins, CO 80523 USA. [Klein, Julia A.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Campus Delivery 1472, Ft Collins, CO 80523 USA. [Smith, Melinda D.] Colorado State Univ, Dept Biol, Grad Degree Program Ecol, Campus Delivery 1878, Ft Collins, CO 80523 USA. [La Pierre, Kimberly J.] Univ Calif Berkeley, Dept Integrat Biol, 4099 Valley Life Sci Bldg, Berkeley, CA 94720 USA. RP La Pierre, KJ (reprint author), Yale Univ, Dept Ecol & Evolutionary Biol, 165 Prospect St, New Haven, CT 06511 USA.; La Pierre, KJ (reprint author), Univ Calif Berkeley, Dept Integrat Biol, 4099 Valley Life Sci Bldg, Berkeley, CA 94720 USA. EM kimberly.lapierre@berkeley.edu RI Smith, Melinda/J-8987-2014; OI La Pierre, Kimberly/0000-0001-7056-4547 FU NSF [NSF-DEB-1042132]; Yale Institute for Biospheric Studies Center for Field Ecology Pilot Grant; Konza Prairie LTER [NSF-DEB-0823341]; Shortgrass Steppe LTER [NSF-DEB-1027319] FX Thanks are due to M. Avolio, C. Blair, C. Chang, C. Davis, L. Dev, K. Harmony, J. Kray, S. Koerner, A. Kuhl, A. Monty, P. O'Neil, M. Parsons, R. Ramundo, T. Schreck, and the SGS field crew for field assistance. M. Avolio, K. Gross, D. Post, O. Schmitz, and two anonymous reviewers provided helpful feedback on earlier drafts of the manuscript. K. La Pierre was supported by an NSF Graduate Research Fellowship. Additional funding was provided by a Yale Institute for Biospheric Studies Center for Field Ecology Pilot Grant to K. La Pierre, Konza Prairie LTER (NSF-DEB-0823341), and Shortgrass Steppe LTER (NSF-DEB-1027319). This work was generated using data from three sites within the Nutrient Network, coordinated through Research Coordination Network funding from NSF to E. Borer and E. Seabloom (NSF-DEB-1042132). Nitrogen fertilizer was graciously donated by Crop Production Services, Loveland, CO. NR 63 TC 1 Z9 1 U1 9 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD APR PY 2016 VL 19 IS 3 BP 521 EP 533 DI 10.1007/s10021-015-9949-7 PG 13 WC Ecology SC Environmental Sciences & Ecology GA DH8BL UT WOS:000373018200011 ER PT J AU Westfall, JA AF Westfall, James A. TI Strategies for the use of mixed-effects models in continuous forest inventories SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Forest monitoring; Subsampling; Tree height model; Random parameter; Prediction bias ID HEIGHT-DIAMETER MODEL; NORTHWESTERN SPAIN; UNITED-STATES; AGED STANDS; TREES; PINE; PREDICTION; REGRESSION; PLANTATIONS; CURVES AB Forest inventory data often consists of measurements taken on field plots as well as values predicted from statistical models, e.g., tree biomass. Many of these models only include fixed-effects parameters either because at the time the models were established, mixed effects model theory had not yet been thoroughly developed or the use of mixed models was deemed unnecessary or too complex. Over the last two decades, considerable research has been conducted on the use of mixed models in forestry, such that mixed models and their applications are generally well understood. However, most of these assessments have focused on static validation data, and mixed model applications in the context of continuous forest inventories have not been evaluated. In comparison to fixed-effects models, the results of this study showed that mixed models can provide considerable reductions in prediction bias and variance for the population and also for subpopulations therein. However, the random effects resulting from the initial model fit deteriorated rapidly over time, such that some field data is needed to effectively recalibrate the random effects for each inventory cycle. Thus, implementation of mixed models requires ongoing maintenance to reap the benefits of improved predictive behavior. Forest inventory managers must determine if this gain in predictive power outweighs the additional effort needed to employ mixed models in a temporal framework. C1 [Westfall, James A.] US Forest Serv, No Res Stn, Newtown Sq, PA USA. RP Westfall, JA (reprint author), US Forest Serv, No Res Stn, Newtown Sq, PA USA. EM jameswestfall@fs.fed.us NR 30 TC 1 Z9 1 U1 3 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD APR PY 2016 VL 188 IS 4 DI 10.1007/s10661-016-5252-0 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH4IW UT WOS:000372750100044 PM 27010710 ER PT J AU Brown, DW Proctor, RH AF Brown, Daren W. Proctor, Robert H. TI Insights into natural products biosynthesis from analysis of 490 polyketide synthases from Fusarium SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Fusarium; Secondary metabolite; Natural product; Polyketide; Evolution; Polyketide synthase ID NONRIBOSOMAL SYNTHETASE GENES; RED PIGMENT BIKAVERIN; GIBBERELLA-ZEAE; ASPERGILLUS-NIDULANS; SECONDARY METABOLISM; HORIZONTAL TRANSFER; CLUSTER; GRAMINEARUM; FUJIKUROI; FUNGUS AB Species of the fungus Fusarium collectively cause disease on almost all crop plants and produce numerous natural products (NPs), including some of the mycotoxins of greatest concern to agriculture. Many Fusarium NPs are derived from polyketide synthases (PKSs), large multi-domain enzymes that catalyze sequential condensation of simple carboxylic acids to form polyketides. To gain insight into the biosynthesis of polyketide-derived NPs in Fusarium, we retrieved 488 PKS gene sequences from genome sequences of 31 species of the fungus. In addition to these apparently functional PKS genes, the genomes collectively included 81 pseudogenized PKS genes. Phylogenetic analysis resolved the PKS genes into 67 clades, and based on multiple lines of evidence, we propose that homologs in each Glade are responsible for synthesis of a polyketide that is distinct from those synthesized by PKSs in other clades. The presence and absence of PKS genes among the species examined indicated marked differences in distribution of PKS homologs. Comparisons of Fusarium PKS genes and genes flanking them to those from other Ascomycetes provided evidence that Fusarium has the genetic potential to synthesize multiple NPs that are the same or similar to those reported in other fungi, but that have not yet been reported in Fusarium. The results also highlight ways in which such analyses can help guide identification of novel Fusarium NPs and differences in NP biosynthetic capabilities that exist among fungi. Published by Elsevier Inc. C1 [Brown, Daren W.; Proctor, Robert H.] USDA ARS, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol, 1815 N Univ St, Peoria, IL 61604 USA. RP Brown, DW (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Microbiol, 1815 N Univ St, Peoria, IL 61604 USA. EM daren.brown@ars.usda.gov NR 69 TC 3 Z9 3 U1 13 U2 36 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD APR PY 2016 VL 89 SI SI BP 37 EP 51 DI 10.1016/j.fgb.2016.01.008 PG 15 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA DH3CZ UT WOS:000372666400006 PM 26826610 ER PT J AU Dossett, M Finn, CE AF Dossett, Michael Finn, Chad E. TI Performance and phenology of wild black raspberry (Rubus occidentalis L.) germplasm in a common garden SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Blackcap; Breeding; Cultivar development; Rubus occidentalis; Verticillium ID GENETIC DIVERSITY; INHERITANCE; MARKERS; IDENTIFICATION AB A lack of genetic diversity in cultivated black raspberry (Rubus occidentalis L.) germplasm has been widely recognized as a major factor limiting progress towards breeding improved cultivars. Despite this, little effort has been made since the early twentieth century to systematically collect and evaluate wild black raspberry for germplasm improvement. In recent years, there has been renewed interest in black raspberry breeding to replace existing cultivars that lack durability and disease resistance. We planted seedlings from 109 wild black raspberry populations, representing 24 US states and two Canadian provinces, in the field in replicated trial plots in Corvallis, Oregon (USA), to evaluate their performance. These populations showed wide variation in morphology, architecture, fruiting season, vigor, and apparent field tolerance to Verticillium wilt. For nearly every trait examined, wild black raspberry germplasm exhibited a range of variation beyond existing cultivars, and showed great potential for use in future breeding. While most populations were fairly uniform phenotypically, segregation for fruit gloss and possible tolerance to Verticillium wilt was noted in a few, indicating the possibility of simple inheritance of these traits. A few populations with unusual morphology, such as spinelessness or flower abnormalities, were identified, as were populations that flowered on first year canes and produced fall fruit. Populations from the southern edge of the range appear to be particularly well adapted to western Oregon, with vigorous upright growth, strong canes, and some with a low incidence of Verticillium wilt. This germplasm will be of great value to breeders interested in developing improved black raspberry cultivars. C1 [Dossett, Michael] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Dossett, Michael] Pacific Agri Food Res Ctr, BC Blueberry Council, 6947 7 Hwy,POB 1000, Agassiz, BC V0M 1A0, Canada. [Finn, Chad E.] USDA ARS, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. RP Dossett, M (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. EM Michael.Dossett@agr.gc.ca FU USDA-ARS Northwest Center for Small Fruits Research; USDA-ARS Plant Exchange Office; Oregon Raspberry and Blackberry Commission FX The authors would like to gratefully acknowledge the technical support of Mary Peterson, Ted Mackey, Joe Snead, and Jungmin Lee, as well as the myriad summer students that assisted in data collection and plot maintenance during the course of this project especially Sumi Maristany and Erin Ortiz from the Apprenticeships in Science and Engineering program (Portland, OR). We thank Kim Hummer for her role in organizing and participating in the collection trips. We would like to thank the following individuals for contributing seed for this project: W. Anderson (Illinois), M. Bathrick (Pennsylvania), C. Brown (New Jersey), P. Byers (Missouri), T. Cuff (Wisconsin), A. Dale (Ontario), R. Davis (New York), R. Geneve (Kentucky), J. Hancock (Michigan), D. Handley (Maine), A. Jamieson (New Brunswick), K. Kellogg (Connecticut), J. Lehman (Indiana), T. Leslie (Ohio), H. Love (Tennessee), J. Luby (Minnesota), C. Mauchline (Pennsylvania), R. Moyer (Virginia), G. Nonnecke (Iowa), M. Retter (Indiana), M. Stanton (Ohio), H. Swartz (Maryland), F. Takeda (West Virginia), E. Thompson (Arkansas), C. Weber (New York). This project was supported by grants from the USDA-ARS Northwest Center for Small Fruits Research, the USDA-ARS Plant Exchange Office, and the Oregon Raspberry and Blackberry Commission. NR 30 TC 0 Z9 0 U1 6 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD APR PY 2016 VL 63 IS 4 BP 653 EP 673 DI 10.1007/s10722-015-0274-y PG 21 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DH9LC UT WOS:000373116600007 ER PT J AU Ulloa, M Wang, C Saha, S Hutmacher, RB Stelly, DM Jenkins, JN Burke, J Roberts, PA AF Ulloa, M. Wang, C. Saha, S. Hutmacher, R. B. Stelly, D. M. Jenkins, J. N. Burke, J. Roberts, P. A. TI Analysis of root-knot nematode and fusarium wilt disease resistance in cotton (Gossypium spp.) using chromosome substitution lines from two alien species SO GENETICA LA English DT Article DE Disease resistance; Upland cotton; Pima cotton; Resistance genes; Alleles; Quantitative trait loci; QTL mapping; Plant survival; Vascular root staining; Root galling index; Nematode egg production ID SIMPLE SEQUENCE REPEAT; F-SP VASINFECTUM; UPLAND COTTON; HIRSUTUM L.; BARBADENSE CHROMOSOME; AGRONOMIC TRAITS; MARKERS; GENE; IDENTIFICATION; INHERITANCE AB Chromosome substitution (CS) lines in plants are a powerful genetic resource for analyzing the contribution of chromosome segments to phenotypic variance. In this study, a series of interspecific cotton (Gossypium spp.) CS lines were used to identify a new germplasm resource, and to validate chromosomal regions and favorable alleles associated with nematode or fungal disease resistance traits. The CS lines were developed in the G. hirsutum L. TM-1 background with chromosome or chromosome segment substitutions from G. barbadense L. Pima 3-79 or G. tomentosum. Root-knot nematode (Meloidogyne incognita) and fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) (races 1 and 4) resistance alleles and quantitative trait loci (QTL) previously placed on cotton chromosomes using SSR markers in two interspecific recombinant inbred line populations were chosen for testing. Phenotypic responses of increased resistance or susceptibility in controlled inoculation and infested field assays confirmed the resistance QTLs, based on substitution with the positive or negative allele for resistance. Lines CS-B22Lo, CS-B04, and CS-B18 showed high resistance to nematode root-galling, confirming QTLs on chromosomes 4 and 22 (long arm) with resistance alleles from Pima 3-79. Line CS-B16 had less fusarium race 1-induced vascular root staining and higher percent survival than the TM-1 parent, confirming a major resistance QTL on chromosome 16. Lines CS-B(17-11) and CS-B17 had high fusarium race 4 vascular symptoms and low survival due to susceptible alleles introgressed from Pima 3-79, confirming the localization on chromosome 17 of an identified QTL with resistance alleles from TM1 and other resistant lines. Analyses validated regions on chromosomes 11, 16, and 17 harboring nematode and fusarium wilt resistance genes and demonstrated the value of CS lines as both a germplasm resource for breeding programs and as a powerful genetic analysis tool for determining QTL effects for disease resistance. CS lines carrying small alien chromosome segments with favorable QTL alleles could be used for effective introgression of biotic stress resistance or many other desirable traits by targeting gene interactions and reducing linkage drag effects. C1 [Ulloa, M.; Burke, J.] USDA ARS, CSRL, Plant Stress & Germplasm Dev Res, PA, 3810 4th St, Lubbock, TX 79415 USA. [Wang, C.; Roberts, P. A.] Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA. [Wang, C.] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Harbin, Peoples R China. [Saha, S.; Jenkins, J. N.] USDA ARS, Mississippi State, MS 39762 USA. [Hutmacher, R. B.] Univ Calif Davis, Dept Plant Sci, Five Points, CA 93624 USA. [Stelly, D. M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. RP Ulloa, M (reprint author), USDA ARS, CSRL, Plant Stress & Germplasm Dev Res, PA, 3810 4th St, Lubbock, TX 79415 USA.; Roberts, PA (reprint author), Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA. EM Mauricio.Ulloa@ars.usda.gov; Philip.roberts@ucr.edu FU USDA-ARS [6208-21000-019-00]; Reimbursable Agreement with Cotton Incorporated (CA State Support Committee), Cary, NC (ARIS Log) [5303-05-00 0029833]; Cooperative Research Agreement with Cotton Incorporated and a University of California Discovery Grant (PAR) FX This study was funded by USDA-ARS (Project 6208-21000-019-00) (MU and JB) and partially funded by a Reimbursable Agreement with Cotton Incorporated (CA State Support Committee), Cary, NC (ARIS Log Nos. 5303-05-00 0029833) (MU), a Cooperative Research Agreement with Cotton Incorporated and a University of California Discovery Grant (PAR). The authors would like to thank M. Mirzakhmedov and A. Tulanov visiting students from Uzbekistan working at the USDA-ARS, Lubbock, TX for assisting in running some of the SSR markers. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture or University of California. The U. S. Department of Agriculture and University of California are equal opportunity providers and employers. NR 39 TC 0 Z9 0 U1 5 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 EI 1573-6857 J9 GENETICA JI Genetica PD APR PY 2016 VL 144 IS 2 BP 167 EP 179 DI 10.1007/s10709-016-9887-0 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA DH9BY UT WOS:000373092000004 PM 26882892 ER PT J AU Leroux, DJ Das, NN Entekhabi, D Colliander, A Njoku, E Jackson, TJ Yueh, S AF Leroux, Delphine J. Das, Narendra N. Entekhabi, Dara Colliander, Andreas Njoku, Eni Jackson, Thomas J. Yueh, Simon TI Active-Passive Soil Moisture Retrievals During the SMAP Validation Experiment 2012 SO IEEE GEOSCIENCE AND REMOTE SENSING LETTERS LA English DT Article DE Active-passive; brightness temperature disaggregation; SMAP; soil moisture ID RADIOMETER; SMAPVEX12; RADAR AB The goal of this study is to assess the performance of the active-passive algorithm for the NASA Soil Moisture Active Passive mission (SMAP) using airborne and ground observations from a field campaign. The SMAP active-passive algorithm disaggregates the coarse-resolution radiometer brightness temperature (TB) using high-resolution radar backscatter (sigma(o)) observations. The colocated TB and sigma(o) acquired by the aircraft-based Passive Active L-and S-band sensor during the SMAP Validation Experiment 2012 (SMAPVEX12) are used to evaluate this algorithm. The estimation of its parameters is affected by changes in vegetation during the campaign. Key features of the campaign were the wide range of vegetation growth and soil moisture conditions during the experiment period. The algorithm performance is evaluated by comparing retrieved soil moisture from the disaggregated brightness temperatures to in situ soil moisture measurements. A minimum performance algorithm is also applied, where the radar data are withheld. The minimum performance algorithm serves as a benchmark to asses the value of the radar to the SMAP active-passive algorithm. The temporal correlation between ground samples and the SMAP active-passive algorithm is improved by 21% relative to minimum performance. The unbiased root-mean-square error is decreased by 15% overall. C1 [Leroux, Delphine J.; Das, Narendra N.; Colliander, Andreas; Njoku, Eni; Yueh, Simon] CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91125 USA. [Leroux, Delphine J.] Ctr Etud Spatiales Biosphere CESBIO, F-31400 Toulouse, France. [Entekhabi, Dara] MIT, 77 Massachusetts Ave, Cambridge, MA 02139 USA. [Jackson, Thomas J.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Das, NN (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91125 USA. EM nndas@jpl.nasa.gov NR 12 TC 4 Z9 4 U1 9 U2 24 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1545-598X EI 1558-0571 J9 IEEE GEOSCI REMOTE S JI IEEE Geosci. Remote Sens. Lett. PD APR PY 2016 VL 13 IS 4 BP 475 EP 479 DI 10.1109/LGRS.2015.2491643 PG 5 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA DH7YJ UT WOS:000373009800001 ER PT J AU Handler, AM AF Handler, Alfred M. TI Enhancing the stability and ecological safety of mass-reared transgenic strains for field release by redundant conditional lethality systems SO INSECT SCIENCE LA English DT Review DE conditional lethality; ecological safety; redundant lethality; Sterile Insect Technique; transgenic insects ID STERILE INSECT TECHNIQUE; MEDITERRANEAN FRUIT-FLY; DROSOPHILA-MELANOGASTER; ANASTREPHA-SUSPENSA; CERATITIS-CAPITATA; GENETIC SYSTEM; POPULATION-CONTROL; TRANSPOSON VECTOR; TRANSFORMER GENE; SEXING SYSTEM AB The genetic manipulation of agriculturally important insects now allows the development of genetic sexing and male sterility systems for more highly efficient biologically-based population control programs, most notably the Sterile Insect Technique (SIT), for both plant and animal insect pests. Tetracycline-suppressible (Tet-off) conditional lethal systems may function together so that transgenic strains will be viable and fertile on a tetracycline-containing diet, but female-lethal and male sterile in tetracycline-free conditions. This would allow their most efficacious use in a unified system for sterile male-only production for SIT. A critical consideration for the field release of such transgenic insect strains, however, is a determination of the frequency and genetic basis of lethality revertant survival. This will provide knowledge essential to evaluating the genetic stability of the lethality system, its environmental safety, and provide the basis for modifications ensuring optimal efficacy. For Tet-off lethal survival determinations, development of large-scale screening protocols should also allow the testing of these modifications, and test the ability of other conditional lethal systems to fully suppress propagation of rare Tet-off survivors. If a dominant temperature sensitive (DTS) pupal lethality system proves efficient for secondary lethality in Drosophila, it may provide the safeguard needed to support the release of sexing/sterility strains, and potentially, the release of unisex lethality strains as a form of genetic male sterility. Should the DTS Pros2(1) mutation prove effective for redundant lethality, its high level of structural and functional conservation should allow host-specific cognates to be created for a wide range of insect species. C1 [Handler, Alfred M.] ARS, USDA, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. RP Handler, AM (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM al.handler@ars.usda.gov NR 49 TC 1 Z9 1 U1 3 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD APR PY 2016 VL 23 IS 2 BP 225 EP 234 DI 10.1111/1744-7917.12245 PG 10 WC Entomology SC Entomology GA DH7YO UT WOS:000373010300007 PM 26097098 ER PT J AU Martin, JA Garczynski, SF AF Martin, Jessica A. Garczynski, Stephen F. TI Putative nicotinic acetylcholine receptor subunits express differentially through the life cycle of codling moth, Cydia pomonella (Lepidoptera: Tortricidae) SO INSECT SCIENCE LA English DT Article DE codling moth; nAChR expression; nicotinic acetylcholine receptor subunits ID TARGET-SITE RESISTANCE; GENE FAMILY; DROSOPHILA-MELANOGASTER; SPINOSAD RESISTANCE; LOOP-B; MECHANISMS; CONSEQUENCES; TRANSCRIPTS; SENSITIVITY; PROGRAMS AB Nicotinic acetylcholine receptors (nAChRs) are the targets of neonicotinoids and spinosads, two insecticides used in orchards to effectively control codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). Orchardists in Washington State are concerned about the possibility of codling moth field populations developing resistance to these two insecticides. In an effort to help mitigate this issue, we initiated a project to identify and characterize codling moth nAChR subunits expressed in heads. This study had two main goals; (i) identify transcripts from a codling moth head transcriptome that encode for nAChR subunits, and (ii) determine nAChR subunit expression profiles in various life stages of codling moth. From a codling moth head transcriptome, 24 transcripts encoding for 12 putative nAChR subunit classes were identified and verified by PCR amplification, cloning, and sequence determination. Characterization of the deduced protein sequences encoded by putative nAChR transcripts revealed that they share the distinguishing features of the cys-loop ligand-gated ion channel superfamily with 9 -type subunits and 3 -type subunits identified. Phylogenetic analysis comparing these protein sequences to those of other insect nAChR subunits supports the identification of these proteins as nAChR subunits. Stage expression studies determined that there is clear differential expression of many of these subunits throughout the codling moth life cycle. The information from this study will be used in the future to monitor for potential target-site resistance mechanisms to neonicotinoids and spinosads in tolerant codling moth populations. C1 [Martin, Jessica A.; Garczynski, Stephen F.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Garczynski, SF (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM steve.garczynski@ars.usda.gov FU Washington Tree Fruit Research Commission [CP-13-101]; Research Experience for Undergraduates program of the National Science Foundation (NSF-REU) [1156603] FX We thank Drs. Man-Yeon Choi and Rodney Cooper for critical review of this manuscript. We are most grateful to Laura Willett, Kim Shannon, and Karolynn Tom for maintenance of the codling moth colony. Dr. Michael Parra (Heritage University) is thanked for his helpful discussions and comments. This work was supported by a grant from the Washington Tree Fruit Research Commission (awarded to SFG, CP-13-101) and a grant from the Research Experience for Undergraduates program of the National Science Foundation (awarded to Nina Barcenas, Heritage University, NSF-REU Award# 1156603). NR 43 TC 0 Z9 1 U1 4 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD APR PY 2016 VL 23 IS 2 BP 277 EP 287 DI 10.1111/1744-7917.12196 PG 11 WC Entomology SC Entomology GA DH7YO UT WOS:000373010300012 PM 25504620 ER PT J AU Ortega-Beltran, A Grubisha, LC Callicott, KA Cotty, PJ AF Ortega-Beltran, A. Grubisha, L. C. Callicott, K. A. Cotty, P. J. TI The vegetative compatibility group to which the US biocontrol agent Aspergillus flavus AF36 belongs is also endemic to Mexico SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE atoxigenic; biopesticide registration; gene cluster degeneration; linkage disequilibrium; microsatellite loci; North American Free Trade Agreement; vegetative compatibility ID REDUCE AFLATOXIN CONTAMINATION; BIOSYNTHESIS GENE-CLUSTER; BIOLOGICAL-CONTROL; FIELD APPLICATION; FLANKING REGIONS; PARASEXUAL CYCLE; RISK-ASSESSMENT; UNITED-STATES; MAIZE; CORN AB AimsTo assess frequencies of the Aspergillus flavus atoxigenic vegetative compatibility group (VCG) YV36, to which the biocontrol agent AF36 belongs, in maize-growing regions of Mexico. Methods and ResultsOver 3500 A.flavus isolates recovered from maize agroecosystems in four states of Mexico during 2005 through 2008 were subjected to vegetative compatibility analyses based on nitrate nonutilizing mutants. Results revealed that 59 (16%) isolates belong to VCG YV36. All 59 isolates had the MAT1-2 idiomorph at the mating-type locus and the single nucleotide polymorphism in the polyketide synthase gene that confers atoxigenicity. Additional degradation of the aflatoxin gene cluster was detected in three isolates. Microsatellite loci analyses revealed low levels of genetic diversity and no linkage disequilibrium within VCG YV36. ConclusionsThe VCG to which the biocontrol agent AF36 belongs, YV36, is also native to Mexico. The North American Free Trade Agreement should facilitate adoption of AF36 for use by Mexico in aflatoxin prevention programs. Significance and Impact of the StudyAn USEPA registered biocontrol agent effective at preventing aflatoxin contamination of crops in the US, is also native to Mexico. This should facilitate the path to registration of AF36 as the first biopesticide for aflatoxin mitigation of maize in Mexico. Economic and health benefits to the population of Mexico should result once aflatoxin mitigation programs based on AF36 applications are implemented. C1 [Ortega-Beltran, A.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Grubisha, L. C.] Univ Wisconsin, Dept Nat & Appl Sci, Green Bay, WI 54302 USA. [Callicott, K. A.; Cotty, P. J.] Univ Arizona, USDA ARS, Sch Plant Sci, POB 21003, Tucson, AZ 85721 USA. [Ortega-Beltran, A.] IITA, PMB 5320, Ibadan, Nigeria. RP Cotty, PJ (reprint author), Univ Arizona, USDA ARS, Sch Plant Sci, POB 21003, Tucson, AZ 85721 USA. EM pjcotty@email.arizona.edu FU USDA Foreign Agricultural Service; Scientific Cooperation Research Program, USDA [5347-42000-02-00D]; CONACyT-Mexico [309103] FX This study was part of the first author's doctoral dissertation and was funded by the USDA Foreign Agricultural Service, Scientific Cooperation Research Program, USDA Agricultural Research Project 5347-42000-02-00D and CONACyT-Mexico (Scholarship 309103). NR 89 TC 2 Z9 2 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD APR PY 2016 VL 120 IS 4 BP 986 EP 998 DI 10.1111/jam.13047 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DH9IW UT WOS:000373110800015 PM 26744130 ER PT J AU Hansen, ZR Knaus, BJ Tabima, JF Press, CM Judelson, HS Grunwald, NJ Smart, CD AF Hansen, Z. R. Knaus, B. J. Tabima, J. F. Press, C. M. Judelson, H. S. Gruenwald, N. J. Smart, Christine D. TI Loop-mediated isothermal amplification for detection of the tomato and potato late blight pathogen, Phytophthora infestans SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE agriculture; diagnostics; late blight; loop-mediated isothermal amplification; Phytophthora infestans; plant pathology; potato; tomato ID RAPID DETECTION; RIBOSOMAL DNA; SPACER REGION; PCR; LAMP; IDENTIFICATION; ASSAY; ORGANIZATION; RESISTANCE; SEQUENCES AB AimsTo design and validate a colorimetric loop-mediated isothermal amplification assay for rapid detection of Phytophthora infestans DNA. Methods and ResultsTwo sets of loop-mediated isothermal amplification (LAMP) primers were designed and evaluated for their sensitivity and specificity for P.infestans. ITSII primers targeted a portion of the internal transcribed spacer region of ribosomal DNA. These primers had a limit of detection of 2pg P.infestans DNA and cross-reacted with the closely related species Phytophthora nicotianae. Rgn86_2 primers, designed to improve assay specificity, targeted a portion of a conserved hypothetical protein. These primers had a limit of detection of 200pg P.infestans DNA and did not cross-react with P.nicotianae. The specificity of the Rgn86_2 assay was tested further using the closely related species P.andina, P.ipomoeae, P.mirabilis and P.phaseoli. Cross-reactions occurred with P.andina and P.mirabilis, but neither species occurs on tomato or potato. Both primer sets were able to detect P.infestans DNA extracted from tomato late blight leaf lesions. ConclusionsTwo colorimetric LAMP assays detected P.infestans DNA from pure cultures as well as infected leaf tissue. The ITSII primers had higher sensitivity, and the Rgn86_2 primers had higher specificity. Significance and Impact of the StudyThis is the first report of a LAMP assay for the detection of P.infestans, the causal organism of potato and tomato late blight. These assays have potential for immediate utility in plant disease research and diagnostic laboratories. C1 [Hansen, Z. R.; Smart, Christine D.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. [Knaus, B. J.; Press, C. M.; Gruenwald, N. J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Tabima, J. F.; Gruenwald, N. J.] Oregon State Univ, Bot & Plant Pathol, Corvallis, OR 97331 USA. [Judelson, H. S.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. RP Smart, CD (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. EM cds14@cornell.edu OI Grunwald, Niklaus/0000-0003-1656-7602 FU US Department of Agriculture (USDA) Agricultural Research Service Grant [5358-22000-039-00D]; USDA National Institute of Food and Agriculture Grant [2011-68004-30154] FX This work was supported in part by US Department of Agriculture (USDA) Agricultural Research Service Grant 5358-22000-039-00D (NJG) and USDA National Institute of Food and Agriculture Grant 2011-68004-30154 (HSJ, CDS and NJG). We thank Beth Gugino, Dennis Johnson, Margaret McGrath, Jean Ristaino, Gary Secor and Andrew Wyenandt for providing isolates used in this study. We also thank William Fry and members of his lab for maintaining cultures and providing isolate genotype information and Walter Mahaffee and Lindsey Thiessen for guidance with LAMP protocols and techniques. NR 55 TC 2 Z9 2 U1 5 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD APR PY 2016 VL 120 IS 4 BP 1010 EP 1020 DI 10.1111/jam.13079 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DH9IW UT WOS:000373110800017 PM 26820117 ER PT J AU de Weerd, DRU Robinson, DG Rosenberg, G AF de Weerd, Dennis R. Uit Robinson, David G. Rosenberg, Gary TI Evolutionary and biogeographical history of the land snail family Urocoptidae (Gastropoda: Pulmonata) across the Caribbean region SO JOURNAL OF BIOGEOGRAPHY LA English DT Article DE Antilles; colonization; dispersal; endemic clades; morphological radiation; Urocoptidae; West Indies ID ECOLOGICAL OPPORTUNITY; ADAPTIVE RADIATION; PHYLOGENETIC-RELATIONSHIPS; MOLECULAR PHYLOGENY; DIVERGENCE TIMES; GENUS MANDARINA; OCEANIC ISLANDS; BONIN ISLANDS; WEST-INDIES; BODY-SIZE AB AimLand snails, because of their low vagility, are ideal organisms for probing biogeographical hypotheses. This study is the first to examine the evolutionary and biogeographical history of a group of land snails from the Caribbean region using molecular phylogenetics. It focuses on the family Urocoptidae that displays extraordinary diversity, particularly in the Greater Antilles. LocationCaribbean region. MethodsWe reconstructed the phylogeny of Urocoptidae based on multi-locus (partial 28S, H3 and COI sequences) analyses (MrBayes, BEAST, GARLI) of 65 species, representing 44 recognized genera. Biogeographical analyses of a subset of the time-calibrated BEAST trees were made both with (DEC and DEC+J analysis in BioGeoBEARS) and without (S-DIVA in RASP) palaeogeographical assumptions. In the DEC and DEC+J analyses we examined the effect of different settings for dispersal between directly connected areas relative to that between areas without direct land connection. ResultsUrocoptidae has been present on the Greater Antilles Arc from at least Middle Eocene onwards. Morphologically diverse and previously unrecognized clades evolved on most Caribbean (palaeo)islands. Jamaica was colonized at least twice. Dispersal multiplier matrices with moderately constrained dispersal between areas without direct land connections describe the phylogeographical history of the family with higher DEC and DEC+J lnL scores than uniform matrices. Main conclusionsUrocoptids constitute an old element of the Greater Antillean biota, predating a proposed GAARlandia landspan connection to South America. The biogeographical history and evolution of Urocoptidae were shaped primarily by the geographical distribution of Caribbean landmasses, in combination with occasional oversea dispersal. Oversea dispersal allowed colonization of palaeogeographically isolated areas, such as Jamaica and present-day western Cuba, where presumably the absence of ecological competitors led to independent radiations into similar shell types. C1 [de Weerd, Dennis R. Uit] Open Univ, Fac Management Sci & Technol, Dept Sci, POB 2960, NL-6401 DL Heerlen, Netherlands. [de Weerd, Dennis R. Uit] Netherlands Ctr Biodivers Nat, NL-2300 RA Leiden, Netherlands. [de Weerd, Dennis R. Uit; Robinson, David G.; Rosenberg, Gary] Acad Nat Sci Philadelphia, Dept Malacol, Philadelphia, PA 19130 USA. [Robinson, David G.] Acad Nat Sci Philadelphia, Dept Malacol, USDA APHIS PPQ, Philadelphia, PA 19130 USA. RP de Weerd, DRU (reprint author), Open Univ, Fac Management Sci & Technol, Dept Sci, POB 2960, NL-6401 DL Heerlen, Netherlands. EM dennis.uitdeweerd@ou.nl FU Laboratory for Molecular Systematics and Ecology at ANSP FX Wolter Moes, Alfredo Gutierrez, Antonio Vazquez Perera, Alejandro Fernandez, Adrian Gonzalez Guillen and Iradys Caballero provided crucial help with fieldwork in Cuba. Igor Muratov helped collecting Jamaican and Hispaniolan samples. John Slapcinsky (Florida Museum of Natural History), Jozef Grego and Jozef Steffek kindly provided specimens used in this study. We thank Anthony Geneva for help and advice in the lab. This work was funded in part by a gift from I. Wistar Morris that established the Laboratory for Molecular Systematics and Ecology at ANSP. The manuscript benefitted from comments by three anonymous referees. NR 97 TC 0 Z9 0 U1 11 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0305-0270 EI 1365-2699 J9 J BIOGEOGR JI J. Biogeogr. PD APR PY 2016 VL 43 IS 4 BP 763 EP 777 DI 10.1111/jbi.12692 PG 15 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA DH9ZH UT WOS:000373154000011 ER PT J AU Haley, NJ Siepker, C Walter, WD Thomsen, BV Greenlee, JJ Lehmkuhl, AD Richt, JA AF Haley, Nicholas J. Siepker, Chris Walter, W. David Thomsen, Bruce V. Greenlee, Justin J. Lehmkuhl, Aaron D. Richt, Juergen A. TI Antemortem Detection of Chronic Wasting Disease Prions in Nasal Brush Collections and Rectal Biopsy Specimens from White-Tailed Deer by Real-Time Quaking-Induced Conversion SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID CERVUS-ELAPHUS-NELSONI; ROCKY-MOUNTAIN ELK; CAPTIVE MULE DEER; ODOCOILEUS-VIRGINIANUS; SPONGIFORM ENCEPHALOPATHY; CYCLIC AMPLIFICATION; DIAGNOSTIC-ACCURACY; CLASSICAL SCRAPIE; PREVALENCE; PROTEIN AB Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of cervids, was first documented nearly 50 years ago in Colorado and Wyoming and has since spread to cervids in 23 states, two Canadian provinces, and the Republic of Korea. The expansion of this disease makes the development of sensitive diagnostic assays and antemortem sampling techniques crucial for the mitigation of its spread; this is especially true in cases of relocation/reintroduction of farmed or free-ranging deer and elk or surveillance studies of private or protected herds, where depopulation is contraindicated. This study sought to evaluate the sensitivity of the real-time quaking-induced conversion (RT-QuIC) assay by using recto-anal mucosa-associated lymphoid tissue (RAMALT) biopsy specimens and nasal brush samples collected antemortem from farmed white-tailed deer (n = 409). Antemortem findings were then compared to results from ante-and postmortem samples (RAMALT, brainstem, and medial retropharyngeal lymph nodes) evaluated by using the current gold standard in vitro assay, immunohistochemistry (IHC) analysis. We hypothesized that the sensitivity of RT-QuIC would be comparable to IHC analysis in antemortem tissues and would correlate with both the genotype and the stage of clinical disease. Our results showed that RAMALT testing by RT-QuIC assay had the highest sensitivity (69.8%) compared to that of postmortem testing, with a specificity of > 93.9%. These data suggest that RT-QuIC, like IHC analysis, is an effective assay for detection of PrPCWD in rectal biopsy specimens and other antemortem samples and, with further research to identify more sensitive tissues, bodily fluids, or experimental conditions, has potential for large-scale and rapid automated testing for CWD diagnosis. C1 [Haley, Nicholas J.; Siepker, Chris; Richt, Juergen A.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. [Walter, W. David] Penn State Univ, US Geol Survey, Penn Cooperat Fish & Wildlife Res Unit, University Pk, PA 16802 USA. [Thomsen, Bruce V.; Lehmkuhl, Aaron D.] APHIS, USDA, VS, STAS,Natl Vet Serv Labs, Ames, IA USA. [Greenlee, Justin J.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Haley, Nicholas J.] Midwestern Univ, Dept Microbiol & Immunol, Glendale, AZ USA. RP Haley, NJ (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA.; Haley, NJ (reprint author), Midwestern Univ, Dept Microbiol & Immunol, Glendale, AZ USA. EM nicholas.j.haley@gmail.com FU North American Deer Farmers Association; Merial; HHS \ NIH \ National Center for Research Resources (NCRR) [NIH NCRR K01OD010994] FX North American Deer Farmers Association provided funding to Nicholas J. Haley. Merial provided funding to Chris Siepker. HHS vertical bar NIH vertical bar National Center for Research Resources (NCRR) provided funding to Nicholas J. Haley under grant number NIH NCRR K01OD010994. NR 47 TC 2 Z9 2 U1 5 U2 11 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD APR PY 2016 VL 54 IS 4 BP 1108 EP 1116 DI 10.1128/JCM.02699-15 PG 9 WC Microbiology SC Microbiology GA DI2OX UT WOS:000373337100036 PM 26865693 ER PT J AU Wang, Y Kaftanoglu, O Brent, CS Page, RE Amdam, GV AF Wang, Ying Kaftanoglu, Osman Brent, Colin S. Page, Robert E., Jr. Amdam, Gro V. TI Starvation stress during larval development facilitates an adaptive response in adult worker honey bees (Apis mellifera L.) SO JOURNAL OF EXPERIMENTAL BIOLOGY LA English DT Article DE Nutrition; Eusocial species; Anticipatory mechanism; Stress response; Adaptive response; Division of labor; Glucose; Trehalose; Glycogen; Lipid ID DIVISION-OF-LABOR; INSULIN-LIKE PEPTIDES; TIME RT-PCR; BUTTERFLY BICYCLUS-ANYNANA; JUVENILE-HORMONE TITERS; CATCH-UP GROWTH; BEHAVIORAL-DEVELOPMENT; CASTE DEVELOPMENT; SOCIAL-BEHAVIOR; GENE-EXPRESSION AB Most organisms are constantly faced with environmental changes and stressors. In diverse organisms, there is an anticipatory mechanism during development that can program adult phenotypes. The adult phenotype would be adapted to the predicted environment that occurred during organism maturation. However, whether this anticipatory mechanism is present in eusocial species is questionable because eusocial organisms are largely shielded from exogenous conditions by their stable nest environment. In this study, we tested whether food deprivation during development of the honey bee (Apis mellifera), a eusocial insect model, can shift adult phenotypes to better cope with nutritional stress. After subjecting fifth instar worker larvae to short-term starvation, we measured nutrition-related morphology, starvation resistance, physiology, endocrinology and behavior in the adults. We found that the larval starvation caused adult honey bees to become more resilient toward starvation. Moreover, the adult bees were characterized by reduced ovary size, elevated glycogen stores and juvenile hormone (JH) titers, and decreased sugar sensitivity. These changes, in general, can help adult insects survive and reproduce in food-poor environments. Overall, we found for the first time support for an anticipatory mechanism in a eusocial species, the honey bee. Our results suggest that this mechanism may play a role in honey bee queen-worker differentiation and worker division of labor, both of which are related to the responses to nutritional stress. C1 [Wang, Ying; Kaftanoglu, Osman; Page, Robert E., Jr.; Amdam, Gro V.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Brent, Colin S.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Page, Robert E., Jr.] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 96616 USA. [Page, Robert E., Jr.] Santa Fe Inst, Santa Fe, NM 87501 USA. [Amdam, Gro V.] Norwegian Univ Life Sci, Dept Ecol & Nat Resource Management, N-1432 As, Norway. RP Wang, Y (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. EM ying.wang.6@asu.edu OI Brent, Colin/0000-0003-2078-1417 FU Research Council of Norway [180504, 185306]; PEW Charitable Trust FX This research was supported by the Research Council of Norway [180504, 185306] and The PEW Charitable Trust to G.V.A. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 141 TC 2 Z9 2 U1 17 U2 34 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0022-0949 EI 1477-9145 J9 J EXP BIOL JI J. Exp. Biol. PD APR 1 PY 2016 VL 219 IS 7 BP 949 EP 959 DI 10.1242/jeb.130435 PG 11 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DI0UK UT WOS:000373212600014 PM 27030775 ER PT J AU Panagos, PG Vishwanathan, R Penfield-Cyr, A Matthan, NR Shivappa, N Wirth, MD Hebert, JR Sen, S AF Panagos, P. G. Vishwanathan, R. Penfield-Cyr, A. Matthan, N. R. Shivappa, N. Wirth, M. D. Hebert, J. R. Sen, S. TI Breastmilk from obese mothers has pro-inflammatory properties and decreased neuroprotective factors SO JOURNAL OF PERINATOLOGY LA English DT Article ID ALPHA-TOCOPHEROL; BODY-COMPOSITION; ENERGY-INTAKE; FATTY-ACIDS; INDEX; PREGNANCY; WEIGHT; WOMEN; VALIDATION; OVERWEIGHT AB OBJECTIVE: To determine the impact of maternal obesity on breastmilk composition. STUDY DESIGN: Breastmilk and food records from 21 lean and 21 obese women who delivered full-term infants were analyzed at 2 months post-partum. Infant growth and adiposity were measured at birth and 2 months of age. RESULT: Breastmilk from obese mothers had higher omega-6 to omega-3 fatty acid ratio and lower concentrations of docosahexaenoic acid, eicosapentaenoic acid, docasapentaenoic acid and lutein compared with lean mothers (P < 0.05), which were strongly associated with maternal body mass index. Breastmilk saturated fatty acid and monounsaturated fatty acid concentrations were positively associated with maternal dietary inflammation, as measured by dietary inflammatory index. There were no differences in infant growth measurements. CONCLUSION: Breastmilk from obese mothers has a pro-inflammatory fatty acid profile and decreased concentrations of fatty acids and carotenoids that have been shown to have a critical role in early visual and neurodevelopment. Studies are needed to determine the link between these early-life influences and subsequent cardiometabolic and neurodevelopmental outcomes. C1 [Panagos, P. G.] Floating Hosp Children, Tufts Med Ctr, Boston, MA USA. [Vishwanathan, R.; Matthan, N. R.; Sen, S.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Penfield-Cyr, A.; Sen, S.] Brigham & Womens Hosp, Dept Pediat Newborn Med, 75 Francis St, Boston, MA 02115 USA. [Shivappa, N.; Wirth, M. D.; Hebert, J. R.] Univ S Carolina, Dept Epidemiol & Biostat, Canc Prevent & Control Program, Columbia, SC 29208 USA. [Shivappa, N.; Wirth, M. D.; Hebert, J. R.] Connecting Hlth Innovat LLC, Columbia, SC USA. RP Sen, S (reprint author), Brigham & Womens Hosp, Dept Newborn Med, 75 Francis St, Boston, MA 02115 USA. EM ssen2@partners.org FU Tufts Medical Center Department of Pediatrics; Susan Saltonstall Foundation; NICHD [K23HD074648]; NIDDK [R44DK103377] FX This work is supported by Tufts Medical Center Department of Pediatrics, Susan Saltonstall Foundation Grant Program, Pilot Project Award 2012, NICHD K23HD074648 (SS), NIDDK R44DK103377 (NS, MDW and JRH). NR 46 TC 1 Z9 1 U1 4 U2 4 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0743-8346 EI 1476-5543 J9 J PERINATOL JI J. Perinatol. PD APR PY 2016 VL 36 IS 4 BP 284 EP 290 DI 10.1038/jp.2015.199 PG 7 WC Obstetrics & Gynecology; Pediatrics SC Obstetrics & Gynecology; Pediatrics GA DH5UL UT WOS:000372857400008 PM 26741571 ER PT J AU Bahmani, P van de Lindt, JW Gershfeld, M Mochizuki, GL Pryor, SE Rammer, D AF Bahmani, Pouria van de Lindt, John W. Gershfeld, Mikhail Mochizuki, Gary L. Pryor, Steven E. Rammer, Douglas TI Experimental Seismic Behavior of a Full-Scale Four-Story Soft-Story Wood-Frame Building with Retrofits. I: Building Design, Retrofit Methodology, and Numerical Validation SO JOURNAL OF STRUCTURAL ENGINEERING LA English DT Article DE Performance-based seismic design; FEMA P-807; Soft-story; Wood frame; Shake table; Seismic retrofit; Wood structures AB Soft-story wood-frame buildings have been recognized as a disaster preparedness problem for decades. There are tens of thousands of these multifamily three- and four-story structures throughout California and other parts of the United States. The majority were constructed between 1920 and 1970 and are prevalent in regions such as the San Francisco Bay Area in California. The NEES-Soft project was a five-university multiindustry effort that culminated in a series of full-scale soft-story wood-frame building tests to validate retrofit philosophies proposed by (1)Federal Emergency Management Agency's recent soft-story seismic retrofit guideline for wood buildings and (2)a performance-based seismic retrofit (PBSR) approach developed as part of the NEES-Soft project. This paper is the first in a set of companion papers that presents the building design, retrofit objectives and designs, and full-scale shake table test results of a four-story 370-m2 (4,000-ft2) soft-story test building. Four different retrofit designs were developed and tested at full scale, each with specified performance objectives, which were typically not the same. Three of these retrofits were stiffness or strength-based strategies and one applied supplemental damping devices in a pinned preassembled frame. This paper focuses on the building and retrofit design methodologies and specifics and the companion paper presents the experimental results of full-scale shake table tests ranging from 0.2- to 1.8-g spectral acceleration for all four retrofits. (C) 2014 American Society of Civil Engineers. C1 [Bahmani, Pouria] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [van de Lindt, John W.] Colorado State Univ, Dept Civil & Environm Engn, Infrastruct, Ft Collins, CO 80523 USA. [Gershfeld, Mikhail] Cal Poly, Civil Engn, Pomona, CA 91768 USA. [Mochizuki, Gary L.] Simpson Strong Tie, Pleasanton, CA 94588 USA. [Pryor, Steven E.] Simpson Strong Tie, Bldg Syst, Pleasanton, CA 94588 USA. [Rammer, Douglas] Forest Prod Lab, Madison, WI 53726 USA. RP van de Lindt, JW (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Infrastruct, Ft Collins, CO 80523 USA. EM pbahmani@engr.colostate.edu; jwv@engr.colostate.edu FU National Science Foundation [CMMI-1041631, 1314957]; NEES Operations; Simpson Strong Tie; USDA Forest Products Laboratory; Structural Engineers Association of Southern California; Taylor Devices; Innovative Timber Solutions/Smartwood FX This material is based on work supported by the National Science Foundation under Grant No. CMMI-1041631 and 1314957 (NEES Research) and NEES Operations. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the investigators and do not necessarily reflect the views of the National Science Foundation. A sincere thank you to Simpson Strong Tie for their financial, personnel, and product support throughout the project, including the engineering support for the SMF in San Diego. Cash, personnel, or in-kind contributions were provided by the USDA Forest Products Laboratory, Structural Engineers Association of Southern California, Taylor Devices, and Innovative Timber Solutions/Smartwood. The authors kindly acknowledge the other co-principal investigators (Co-PI's) of the project, Weichiang Pang and Xiaoyun Shao, and other senior personnel of the NEES-Soft project: David V. Rosowsky at University of Vermont, Andre Filiatrault at University at Buffalo, Shiling Pei at South Dakota State University, David Mar at Tipping Mar, and Charles Chadwell at Cal-Poly San Luis Obispo; the other graduate students participating on the project: Ph.D. students Ershad Ziaei (Clemson University) and Jingjing Tian (Rensselaer Polytechnic Institute) and M.Sc. students Jason Au and Robert McDougal at Cal-Poly Pomona; and the practitioner advisory committee: Laurence Kornfield, Tom Van Dorpe, Doug Thompson, Kelly Cobeen, Janiele Maffei, Douglas Taylor, and Rose Grant. A special thank you to all of the research experience for undergraduate (REU) students: Sandra Gutierrez, Faith Silva, Gabriel Banuelos, Rocky Chen, and Connie Tsui. Others that have helped include Asif Iqbal, Andre Barbosa, Vaishak Gopi, Steve Yang, Ed Santos, Tim Ellis, Omar Amini, Russell Ek, Rakesh Gupta, and Anthonie Kramer. Finally, our sincere thank you to NEES and all site staff and site Principal Investigators (PI's) at NEES at the University of California San Diego for their assistance in getting the test specimen ready for testing and in conducting the tests. NR 41 TC 0 Z9 0 U1 5 U2 15 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9445 EI 1943-541X J9 J STRUCT ENG JI J. Struct. Eng. PD APR PY 2016 VL 142 IS 4 SI SI AR E4014003 DI 10.1061/(ASCE)ST.1943-541X.0001207 PG 14 WC Construction & Building Technology; Engineering, Civil SC Construction & Building Technology; Engineering GA DH5EW UT WOS:000372809400005 ER PT J AU Pei, S van de Lindt, JW Popovski, M Berman, JW Dolan, JD Ricles, J Sause, R Blomgren, H Rammer, DR AF Pei, S. van de Lindt, J. W. Popovski, M. Berman, J. W. Dolan, J. D. Ricles, J. Sause, R. Blomgren, H. Rammer, D. R. TI Cross-Laminated Timber for Seismic Regions: Progress and Challenges for Research and Implementation SO JOURNAL OF STRUCTURAL ENGINEERING LA English DT Article AB Forum papers are thought-provoking opinion pieces or essays founded in fact, sometimes containing speculation, on a civil engineering topic of general interest and relevance to the readership of the journal. The views expressed in this Forum article do not necessarily reflect the views of ASCE or the Editorial Board of the journal. C1 [Pei, S.] Colorado Sch Mines, Golden, CO 80403 USA. [van de Lindt, J. W.] Colorado State Univ, Infrastruct, Ft Collins, CO 80523 USA. [Popovski, M.] FP Innovat, Adv Bldg Syst, Vancouver, BC V6T 1Z4, Canada. [Berman, J. W.] Univ Washington, Seattle, WA 98195 USA. [Dolan, J. D.] Washington State Univ, Pullman, WA 99164 USA. [Ricles, J.] Lehigh Univ, Struct Engn, Bethlehem, PA 18015 USA. [Sause, R.] Lehigh Univ, Bethlehem, PA 18015 USA. [Blomgren, H.] Arup, 719 2nd Ave, 400, Seattle, WA 98104 USA. [Rammer, D. R.] Forest Prod Lab, Madison, WI 53726 USA. RP Pei, S (reprint author), Colorado Sch Mines, Golden, CO 80403 USA. EM spei@mines.edu FU National Science Foundation under the George E. Brown Jr. Network for Earthquake Engineering Simulation Research (NEESR) Program [CMMI: 1344617, 1344646, 1344798, 1344590, 1344621] FX The NEES-CLT planning project and the Tall CLT workshop are supported by the National Science Foundation under the George E. Brown Jr. Network for Earthquake Engineering Simulation Research (NEESR) Program. (Awards CMMI: 1344617; 1344646; 1344798; 1344590; 1344621). The authors would also like to thank the Tall CLT Workshop participants for their comments and discussion. Special thanks to Andrew Harmsworth of GHL Consultants, Amie Sullivan of KPFF, Greg Gilda of DCI Engineers, Kris Spickler of Structurlam Products LP, Leif Johnson of Magnusson Klemencic Associates, Phil Line of American Wood Council, Scott Breneman of WoodWorks, Steve Pfeiffer of Seattle DPD, and Steve Pryor of Simpson Strong-Tie Co. for providing valuable input and suggestion to the presented information regarding challenges and opportunities of CLT in the United States. NR 44 TC 0 Z9 0 U1 3 U2 7 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9445 EI 1943-541X J9 J STRUCT ENG JI J. Struct. Eng. PD APR PY 2016 VL 142 IS 4 SI SI AR E2514001 DI 10.1061/(ASCE)ST.1943-541X.0001192 PG 11 WC Construction & Building Technology; Engineering, Civil SC Construction & Building Technology; Engineering GA DH5EW UT WOS:000372809400002 ER PT J AU van de Lindt, JW Bahmani, P Mochizuki, G Pryor, SE Gershfeld, M Tian, JJ Symans, MD Rammer, D AF van de Lindt, John W. Bahmani, Pouria Mochizuki, Gary Pryor, Steven E. Gershfeld, Mikhail Tian, Jingjing Symans, Michael D. Rammer, Douglas TI Experimental Seismic Behavior of a Full-Scale Four-Story Soft-Story Wood-Frame Building with Retrofits. II: Shake Table Test Results SO JOURNAL OF STRUCTURAL ENGINEERING LA English DT Article DE Soft-story; Wood frame; Shake table; Seismic retrofit; Seismic performance; FEMA P-807; Performance-based seismic retrofit; Wood structures AB Soft-story wood-frame buildings have been recognized as a disaster preparedness problem for decades. The majority of these buildings were constructed from the 1920s to the 1960s and are prone to collapse during moderate to large earthquakes due to a characteristic deficiency in strength and stiffness in their first story. In order to propose and validate retrofit methods for these at-risk buildings, a full-scale four-story soft-story wood-frame building was constructed, retrofitted, and subjected to ground motions of various intensities. The tests were conducted to validate retrofit guidelines proposed in a Federal Emergency Management Agency's recent soft-story seismic retrofit guideline for wood buildings and a performance-based seismic retrofit (PBSR) methodology developed as part of the NEES-Soft project. This paper is the second in a set of companion papers and presents the full-scale shake table test results using the two new approaches. The companion paper to this paper presents the design philosophies, design details, and numerical analysis of the retrofitted building for each of the four retrofits. (C) 2014 American Society of Civil Engineers. C1 [van de Lindt, John W.] Colorado State Univ, Dept Civil & Environm Engn, Infrastruct, Ft Collins, CO 80523 USA. [Bahmani, Pouria] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Mochizuki, Gary] Simpson Strong Tie, Pleasanton, CA 94588 USA. [Pryor, Steven E.] Simpson Strong Tie, Bldg Syst, Pleasanton, CA 94588 USA. [Gershfeld, Mikhail] Cal Poly, Civil Engn, Pomona, CA 91768 USA. [Tian, Jingjing; Symans, Michael D.] Rensselaer Polytech Inst, Civil & Environm Engn, Troy, NY 12180 USA. [Rammer, Douglas] Forest Prod Lab, Madison, WI 53726 USA. RP van de Lindt, JW (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Infrastruct, Ft Collins, CO 80523 USA. EM jwv@engr.colostate.edu; pbahmani@engr.colostate.edu FU National Science Foundation [CMMI-1041631, 1314957]; NEES Operations; Simpson Strong Tie; USDA Forest Products Laboratory; Structural Engineers Association of Southern California; Taylor Devices; Innovative Timber Solutions/Smartwood FX This material is based on work supported by the National Science Foundation under Grant No. CMMI-1041631 and 1314957 (NEES Research) and NEES Operations. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the investigators and do not necessarily reflect the views of the National Science Foundation. A sincere thank you to Simpson Strong Tie for their financial, personnel, and product support throughout the project, including the engineering support for the SMF in San Diego. Cash, personnel, or in-kind contributions were provided by the USDA Forest Products Laboratory, Structural Engineers Association of Southern California, Taylor Devices, and Innovative Timber Solutions/Smartwood. The authors kindly acknowledge the other co-principal investigators (Co-PI's) of the project, Weichiang Pang and Xiaoyun Shao, and other senior personnel of the NEES-Soft project: David V. Rosowsky at University of Vermont, Andre Filiatrault at University at Buffalo, Shiling Pei at South Dakota State University, David Mar at Tipping Mar, and Charles Chadwell at Cal-Poly San Luis Obispo; the other graduate students participating on the project: Ph.D. student Ershad Ziaei (Clemson University) and M.Sc. students Jason Au and Robert McDougal at Cal-Poly Pomona; and the practitioner advisory committee: Laurence Kornfield, Tom Van Dorpe, Doug Thompson, Kelly Cobeen, Janiele Maffei, Douglas Taylor, and Rose Grant. A special thank you to all of the research experience for undergraduate (REU) students: Sandra Gutierrez, Faith Silva, Gabriel Banuelos, Rocky Chen, and Connie Tsui. Others that have helped include Asif Iqbal, Andre Barbosa, Vaishak Gopi, Steve Yang, Ed Santos, Tim Ellis, Omar Amini, Russell Ek, Rakesh Gupta, and Anthonie Kramer. Finally, our sincere thank you to NEES and all site staff and site principal investigator (PI's) at NEES at UCSD for their assistance in getting the test specimen ready for testing and in conducting the tests. NR 7 TC 0 Z9 0 U1 3 U2 6 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9445 EI 1943-541X J9 J STRUCT ENG JI J. Struct. Eng. PD APR PY 2016 VL 142 IS 4 SI SI AR E4014004 DI 10.1061/(ASCE)ST.1943-541X.0001206 PG 14 WC Construction & Building Technology; Engineering, Civil SC Construction & Building Technology; Engineering GA DH5EW UT WOS:000372809400004 ER PT J AU Urban, LE Weber, JL Heyman, MB Schichtl, RL Verstraete, S Lowery, NS Das, SK Schleicher, MM Rogers, G Economos, C Masters, WA Roberts, SB AF Urban, Lorien E. Weber, Judith L. Heyman, Melvin B. Schichtl, Rachel L. Verstraete, Sofia Lowery, Nina S. Das, Sai Krupa Schleicher, Molly M. Rogers, Gail Economos, Christina Masters, William A. Roberts, Susan B. TI Energy Contents of Frequently Ordered Restaurant Meals and Comparison with Human Energy Requirements and US Department of Agriculture Database Information: A Multisite Randomized Study SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Dietary energy; Restaurants; Obesity; Fast food; Weight gain ID FAST-FOOD RESTAURANTS; UNITED-STATES; PORTION SIZES; TEMPORAL TRENDS; DIETARY-INTAKE; OBESITY; MENU; CONSUMPTION; SODIUM; ADULTS AB Background Excess energy intake from meals consumed away from home is implicated as a major contributor to obesity, and similar to 50% of US restaurants are individual or small-chain (non-chain) establishments that do not provide nutrition information. Objective To measure the energy content of frequently ordered meals in non-chain restaurants in three US locations, and compare with the energy content of meals from large-chain restaurants, energy requirements, and food database information. Design A multisite random-sampling protocol was used to measure the energy contents of the most frequently ordered meals from the most popular cuisines in non-chain restaurants, together with equivalent meals from large-chain restaurants. Setting Meals were obtained from restaurants in San Francisco, CA; Boston, MA; and Little Rock, AR, between 2011 and 2014. Main outcome measures Meal energy content determined by bomb calorimetry. Statistical analysis performed Regional and cuisine differences were assessed using a mixed model with restaurant nested within region x cuisine as the random factor. Paired t tests were used to evaluate differences between non-chain and chain meals, human energy requirements, and food database values. Results Meals from non-chain restaurants contained 1,205 +/- 465 kcal/meal, amounts that were not significantly different from equivalent meals from large-chain restaurants (+5.1%; P=0.41). There was a significant effect of cuisine on non-chain meal energy, and three of the four most popular cuisines (American, Italian, and Chinese) had the highest mean energy (1,495 kcal/meal). Ninety-two percent of meals exceeded typical energy requirements for a single eating occasion. Conclusions Non-chain restaurants lacking nutrition information serve amounts of energy that are typically far in excess of human energy requirements for single eating occasions, and are equivalent to amounts served by the large-chain restaurants that have previously been criticized for providing excess energy. Restaurants in general, rather than specific categories of restaurant, expose patrons to excessive portions that induce overeating through established biological mechanisms. C1 [Urban, Lorien E.] Gelesis Inc, Boston, MA USA. [Urban, Lorien E.; Lowery, Nina S.; Das, Sai Krupa; Schleicher, Molly M.; Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Energy Metab Lab, 711 Washington St, Boston, MA 02111 USA. [Weber, Judith L.] Univ Arkansas Med Sci, Coll Med, Dept Pediat, Pediat, Little Rock, AR 72205 USA. [Schichtl, Rachel L.] Univ Arkansas Med Sci, Coll Med, Dept Pediat, Little Rock, AR 72205 USA. [Heyman, Melvin B.] Univ Calif San Francisco, Dept Pediat, Pediat Clin, San Francisco, CA USA. [Verstraete, Sofia] Univ Calif San Francisco, Dept Pediat, San Francisco, CA USA. [Lowery, Nina S.] Massachusetts Gen Hosp, Phys Assistant Program, Boston, MA 02114 USA. [Rogers, Gail] Tufts Univ, Jean Mayer US Dept Agr Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Economos, Christina; Masters, William A.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Schleicher, Molly M.] Broad Inst, Off Res Subject Protect, Cambridge, MA USA. RP Roberts, SB (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Energy Metab Lab, 711 Washington St, Boston, MA 02111 USA. EM susan.roberts@tufts.edu RI Masters, William/A-4796-2017 OI Masters, William/0000-0002-4546-7291 FU USDA [58-1950-0-0014, 1950-51000-072-02S]; Tufts University; Arkansas Children's Hospital Research Institute; National Institutes of Health [DK00776] FX Supported by the USDA under agreement nos. 58-1950-0-0014 and 1950-51000-072-02S with Tufts University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. This research was supported, in part, with funding from the Arkansas Children's Hospital Research Institute and in part by National Institutes of Health grant no. DK00776. NR 46 TC 0 Z9 0 U1 4 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD APR PY 2016 VL 116 IS 4 BP 590 EP + DI 10.1016/j.jand.2015.11.009 PG 15 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DH4KJ UT WOS:000372754500006 PM 26803805 ER PT J AU Lee, Y Montgomery, CA Kline, JD AF Lee, Yohan Montgomery, Claire A. Kline, Jeffrey D. TI The influence of age-specific migration on housing growth in the rural Midwest (USA) SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Age-specific migration; Housing density change; Natural amenities; Projection ID FARMLAND PRESERVATION PROGRAMS; QUALITY-OF-LIFE; UNITED-STATES; PUBLIC PREFERENCES; OPEN-SPACE; ECONOMIC-OPPORTUNITY; POPULATION-GROWTH; MOUNTAIN WEST; AMENITIES; URBAN AB Natural resource policymakers and planners increasingly rely on regional and national-level spatial data describing projections of future housing growth, to anticipate development impacts on natural resources and identify policy and planning needs. Such projections have not always been well-grounded in demographic and other factors that influence population and thus housing growth. We develop an empirical model describing population change and housing growth in the rural Midwestern U.S., as a function of demographic transition, socioeconomic factors, and natural amenities. The empirical model is estimated as a set of three equations characterizing: (1) population growth within three age groups, (2) the influence of farmland cover and other county level variables, and (3) household size, housing services, and second home ownership. The estimated population and housing growth models provide a consistent estimate of past change and can be used to project future change. We found age-structure to be an important factor in housing location decisions. Specifically, the influence of natural amenities on both population growth within counties and subsequent housing density changes varies by age group. (C) 2015 Elsevier B.V. All rights reserved. C1 [Lee, Yohan] Yeungnam Univ, Pk Chung Hee Sch Policy & Saemaul, Dept Sustainable Dev, 280 Daehak Ro, Gyongsan 712749, Gyeongsangbuk D, South Korea. [Montgomery, Claire A.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Kline, Jeffrey D.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Lee, Y (reprint author), Yeungnam Univ, Pk Chung Hee Sch Policy & Saemaul, Dept Sustainable Dev, 280 Daehak Ro, Gyongsan 712749, Gyeongsangbuk D, South Korea. EM Yohanlee76@gmail.com; claire.montgomery@oregonstate.edu; jeff.kline@oregonstate.edu FU USDA Forest Service North Central Research Station [03-JV-11231300-065] FX This research was supported by the USDA Forest Service North Central Research Station (joint venture agreement 03-JV-11231300-065). This work does not necessarily reflect the views of the funding agency and no official endorsement should be inferred. We are grateful to Edward A. Stone for contributions to an early version of this manuscript and construction of data sets and preliminary models. We are grateful to Robert Haight for advice. NR 47 TC 0 Z9 0 U1 7 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD APR PY 2016 VL 148 BP 68 EP 79 DI 10.1016/j.landurbplan.2015.12.005 PG 12 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA DH3JR UT WOS:000372683800007 ER PT J AU Diffenderfer, MR Lamon-Fava, S Marcovina, SM Barrett, PHR Lel, J Dolnikowski, GG Berglund, L Schaefer, EJ AF Diffenderfer, Margaret R. Lamon-Fava, Stefania Marcovina, Santica M. Barrett, P. Hugh R. Lel, Julian Dolnikowski, Gregory G. Berglund, Lars Schaefer, Ernst J. TI Distinct metabolism of apolipoproteins (a) and B-100 within plasma lipoprotein(a) SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article DE Lipoprotein(a); Kinetics; Fed state; Hypertriglyceridemia ID B-CONTAINING LIPOPROTEINS; TRIGLYCERIDE-RICH LIPOPROTEINS; CORONARY-HEART-DISEASE; APO-A-I; LOW-DENSITY; SIZE POLYMORPHISM; NICOTINIC-ACID; ISOFORM SIZE; LP(A); CATABOLISM AB Objectives. Lipoprotein(a) [Lp(a)] is mainly similar in composition to LDL, but differs in having apolipoprotein (apo) (a) covalently linked to apoB-100. Our purpose was to examine the individual metabolism of apo(a) and apoB-100 within plasma Lp(a). Materials and Methods. The kinetics of apo(a) and apoB-100 in plasma Lp(a) were assessed in four men with dyslipidemia [Lp(a) concentration: 8.9-124.7 nmol/L]. All subjects received a primed constant infusion of [5,5,5-H-2(3)] L-leucine while in the constantly fed state. Lp(a) was immunoprecipitated directly from whole plasma; apo(a) and apoB-100 were separated by gel electrophoresis; and isotopic enrichment was determined by gas chromatography/mass spectrometry. Results. Multicompartmental modeling analysis indicated that the median fractional catabolic rates of apo(a) and apoB-100 within Lp(a) were significantly different at 0.104 and 0.263 pools/day, respectively (P = 0.04). The median Lp(a) apo(a) production rate at 0.248 nmol/kg . day(-1) was significantly lower than that of Lp(a) apoB-100 at 0.514 nmol/kg . day(-1) (P = 0.03). Conclusion. Our data indicate that apo(a) has a plasma residence time (11 days) that is more than twice as long as that of apoB-100 (4 days) within Lp(a), supporting the concept that apo(a) and apoB-100 within plasma Lp(a) are not catabolized from the bloodstream as a unit in humans in the fed state. (C) 2016 Elsevier Inc. All rights reserved. C1 [Diffenderfer, Margaret R.; Lamon-Fava, Stefania; Lel, Julian; Schaefer, Ernst J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. [Marcovina, Santica M.] Univ Washington, Northwest Lipid Metab & Diabet Res Labs, 401 Queen Anne Ave North, Seattle, WA 98109 USA. [Barrett, P. Hugh R.] Univ Western Australia, Sch Med & Pharmacol, 35 Stirling Highway, Crawley, WA 6009 USA. [Barrett, P. Hugh R.] Univ Western Australia, Fac Engn Comp & Math, 35 Stirling Highway, Crawley, WA 6009 USA. [Dolnikowski, Gregory G.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Mass Spectrometry Unit, 711 Washington St, Boston, MA 02111 USA. [Berglund, Lars] Univ Calif Davis, Clin & Translat Sci Ctr, 2921 Stockton Blvd,Suite 1400, Sacramento, CA 95817 USA. RP Diffenderfer, MR (reprint author), Tufts Univ, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM margaret.diffenderfer@tufts.edu; stefania.lamon-fava@tufts.edu; smm@uw.edu; hugh.barrett@uwa.edu.au; julian.lel@gmail.com; gregory.dolnikowski@tufts.edu; lars.berglund@ucdmc.usdavis.edu; emst.schaefer@tufts.edu OI Barrett, Peter Hugh/0000-0003-3223-6125 FU U.S. Department of Agriculture Research Service [53-3K-06]; National Institutes of Health [P50 HL083813-01, HL62705]; Abbott Laboratories; Abbott Park, IL (US.); Clinical and Translational Research Center of Tufts Medical Center FX This work was supported by U.S. Department of Agriculture Research Service Contract 53-3K-06 (E.J.S.) and by Project Grant P50 HL083813-01 (E.J.S.) and Project Grant HL62705 (L.B.) from the National Institutes of Health. Additional support was provided by an investigator-initiated research grant from Abbott Laboratories, Abbott Park, IL (US.). The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of Abbott Laboratories, the U.S. Department of Agriculture, or the National Institutes of Health. Support for the clinical studies was provided by the Clinical and Translational Research Center of Tufts Medical Center. J.L. was supported by the Harold Williams, M.D. Student Research Fellowship of Tufts University School of Medicine, Boston, MA. P.H.R.B. is a senior research fellow of the National Health and Medical Research Council of Australia. NR 55 TC 4 Z9 4 U1 1 U2 4 PU W B SAUNDERS CO-ELSEVIER INC PI PHILADELPHIA PA 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899 USA SN 0026-0495 EI 1532-8600 J9 METABOLISM JI Metab.-Clin. Exp. PD APR PY 2016 VL 65 IS 4 BP 381 EP 390 DI 10.1016/j.metabol.2015.10.031 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DH3EU UT WOS:000372671100001 PM 26975530 ER PT J AU Hu, Z Zhang, T Gao, XX Wang, Y Zhang, Q Zhou, HJ Zhao, GF Wang, ML Woeste, KE Zhao, P AF Hu, Zhuang Zhang, Tian Gao, Xiao-Xiao Wang, Yang Zhang, Qiang Zhou, Hui-Juan Zhao, Gui-Fang Wang, Ma-Li Woeste, Keith E. Zhao, Peng TI De novo assembly and characterization of the leaf, bud, and fruit transcriptome from the vulnerable tree Juglans mandshurica for the development of 20 new microsatellite markers using Illumina sequencing SO MOLECULAR GENETICS AND GENOMICS LA English DT Article DE Juglans mandshurica; Microsatellites; Transcriptome; Next generation sequencing; Walnut ID EST-SSR MARKERS; RNA-SEQ DATA; POPULATION-STRUCTURE; GENE DISCOVERY; IDENTIFICATION; SOFTWARE; ANNOTATION; VALIDATION; NUCLEAR; WALNUT AB Manchurian walnut (Juglans mandshurica Maxim.) is a vulnerable, temperate deciduous tree valued for its wood and nut, but transcriptomic and genomic data for the species are very limited. Next generation sequencing (NGS) has made it possible to develop molecular markers for this species rapidly and efficiently. Our goal is to use transcriptome information from RNA-Seq to understand development in J. mandshurica and develop polymorphic simple sequence repeats (SSRs, microsatellites) to understand the species' population genetics. In this study, more than 47.7 million clean reads were generated using Illumina sequencing technology. De novo assembly yielded 99,869 unigenes with an average length of 747 bp. Based on sequence similarity search with known proteins, a total of 39,708 (42.32 %) genes were identified. Searching against the Kyoto Encyclopedia of Genes and Genomes Pathway database (KEGG) identified 15,903 (16.9 %) unigenes. Further, we identified and characterized 63 new transcriptome-derived microsatellite markers. By testing the markers on 4 to 14 individuals from four populations, we found that 20 were polymorphic and easily amplified. The number of alleles per locus ranged from 2 to 8. The observed and expected heterozygosity per locus ranged from 0.209 to 0.813 and 0.335 to 0.842, respectively. These twenty microsatellite markers will be useful for studies of population genetics, diversity, and genetic structure, and they will undoubtedly benefit future breeding studies of this walnut species. Moreover, the information uncovered in this research will also serve as a useful genetic resource for understanding the transcriptome and development of J. mandshurica and other Juglans species. C1 [Hu, Zhuang; Zhang, Tian; Gao, Xiao-Xiao; Wang, Yang; Zhang, Qiang; Zhou, Hui-Juan; Zhao, Gui-Fang; Wang, Ma-Li; Zhao, Peng] NW Univ Xian, Coll Life Sci, Xian 710069, Shaanxi, Peoples R China. [Woeste, Keith E.] Purdue Univ, US Forest Serv, Dept Forestry & Nat Resources, USDA,Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. RP Zhao, P (reprint author), NW Univ Xian, Coll Life Sci, Xian 710069, Shaanxi, Peoples R China. EM pengzhao@nwu.edu.cn FU National Natural Science Foundation of China [41471038, 31200500, J1210063]; Program for Excellent Young Academic Backbones by Northwest University [338050070]; Northwest University Training Programs of Innovation and Entrepreneurship for Undergraduates [2015159, 2016171] FX This study was funded by the National Natural Science Foundation of China (Grant No. 41471038, Grant No. 31200500, and Grant No. J1210063), the Program for Excellent Young Academic Backbones funding by Northwest University (Grant No. 338050070), and the Northwest University Training Programs of Innovation and Entrepreneurship for Undergraduates (Grant No. 2015159 and Grand No. 2016171). Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 58 TC 3 Z9 3 U1 12 U2 25 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-4615 EI 1617-4623 J9 MOL GENET GENOMICS JI Mol. Genet. Genomics PD APR PY 2016 VL 291 IS 2 BP 849 EP 862 DI 10.1007/s00438-015-1147-y PG 14 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA DI0AR UT WOS:000373157700025 PM 26614514 ER PT J AU Bertioli, DJ Cannon, SB Froenicke, L Huang, GD Farmer, AD Cannon, EKS Liu, X Gao, DY Clevenger, J Dash, S Ren, LH Moretzsohn, MC Shirasawa, K Huang, W Vidigal, B Abernathy, B Chu, Y Niederhuth, CE Umale, P Araujo, ACG Kozik, A Do Kim, K Burow, MD Varshney, RK Wang, XJ Zhang, XY Barkley, N Guimaraes, PM Isobe, S Guo, BZ Liao, BS Stalker, HT Schmitz, RJ Scheffler, BE Leal-Bertioli, SCM Xun, X Jackson, SA Michelmore, R Ozias-Akins, P AF Bertioli, David John Cannon, Steven B. Froenicke, Lutz Huang, Guodong Farmer, Andrew D. Cannon, Ethalinda K. S. Liu, Xin Gao, Dongying Clevenger, Josh Dash, Sudhansu Ren, Longhui Moretzsohn, Marcio C. Shirasawa, Kenta Huang, Wei Vidigal, Bruna Abernathy, Brian Chu, Ye Niederhuth, Chad E. Umale, Pooja Araujo, Ana Claudia G. Kozik, Alexander Do Kim, Kyung Burow, Mark D. Varshney, Rajeev K. Wang, Xingjun Zhang, Xinyou Barkley, Noelle Guimaraes, Patricia M. Isobe, Sachiko Guo, Baozhu Liao, Boshou Stalker, H. Thomas Schmitz, Robert J. Scheffler, Brian E. Leal-Bertioli, Soraya C. M. Xun, Xu Jackson, Scott A. Michelmore, Richard Ozias-Akins, Peggy TI The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut SO NATURE GENETICS LA English DT Article ID GENETIC-LINKAGE MAP; RNA-SEQ DATA; HYPOGAEA L; B-GENOME; MICROSATELLITE MARKERS; DOMESTICATED PEANUT; QUALITY-CONTROL; WILD RELATIVES; DNA; DIVERGENCE AB Cultivated peanut (Arachis hypogaea) is an allotetraploid with closely related subgenomes of a total size of similar to 2.7 Gb. This makes the assembly of chromosomal pseudomolecules very challenging. As a foundation to understanding the genome of cultivated peanut, we report the genome sequences of its diploid ancestors (Arachis duranensis and Arachis ipaensis). We show that these genomes are similar to cultivated peanut's A and B subgenomes and use them to identify candidate disease resistance genes, to guide tetraploid transcript assemblies and to detect genetic exchange between cultivated peanut's subgenomes. On the basis of remarkably high DNA identity of the A. ipaensis genome and the B subgenome of cultivated peanut and biogeographic evidence, we conclude that A. ipaensis may be a direct descendant of the same population that contributed the B subgenome to cultivated peanut. C1 [Bertioli, David John; Vidigal, Bruna] Univ Brasilia, Inst Biol Sci, Brasilia, DF, Brazil. [Bertioli, David John; Gao, Dongying; Abernathy, Brian; Do Kim, Kyung; Leal-Bertioli, Soraya C. M.; Jackson, Scott A.] Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Cannon, Steven B.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Froenicke, Lutz; Kozik, Alexander; Michelmore, Richard] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Froenicke, Lutz; Michelmore, Richard] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Huang, Guodong; Liu, Xin; Xun, Xu] BGI Shenzhen, Shenzhen, Peoples R China. [Farmer, Andrew D.; Dash, Sudhansu; Umale, Pooja] Natl Ctr Genome Resources, Santa Fe, NM USA. [Cannon, Ethalinda K. S.] Iowa State Univ, Dept Comp Sci, Ames, IA USA. [Clevenger, Josh; Ozias-Akins, Peggy] Univ Georgia, Inst Plant Breeding Genet & Genom, Tifton, GA USA. [Ren, Longhui] Iowa State Univ, Interdept Genet Grad Program, Ames, IA USA. [Moretzsohn, Marcio C.; Vidigal, Bruna; Araujo, Ana Claudia G.; Guimaraes, Patricia M.; Leal-Bertioli, Soraya C. M.] Embrapa Genet Resources & Biotechnol, Brasilia, DF, Brazil. [Shirasawa, Kenta; Isobe, Sachiko] Kazusa DNA Res Inst, Dept Frontier Res, Kisarazu, Japan. [Huang, Wei] Iowa State Univ, Dept Agron, Ames, IA USA. [Chu, Ye; Ozias-Akins, Peggy] Univ Georgia, Dept Hort, Tifton, GA USA. [Niederhuth, Chad E.; Schmitz, Robert J.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. [Burow, Mark D.] Texas A&M AgriLife Res, Lubbock, TX USA. [Burow, Mark D.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. [Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Wang, Xingjun] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan, Peoples R China. [Zhang, Xinyou] Henan Acad Agr Sci, Zhengzhou, Peoples R China. [Barkley, Noelle] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA USA. [Barkley, Noelle] Int Potato Ctr, Lima, Peru. [Guo, Baozhu] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Liao, Boshou] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan, Peoples R China. [Stalker, H. Thomas] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Scheffler, Brian E.] USDA ARS, Middle So Area Genom Lab, Stoneville, MS 38776 USA. RP Bertioli, DJ (reprint author), Univ Brasilia, Inst Biol Sci, Brasilia, DF, Brazil.; Bertioli, DJ (reprint author), Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. EM djbertioli@gmail.com OI Cannon, Steven/0000-0003-2777-8034; Scheffler, Brian/0000-0003-1968-8952 FU Agriculture and Food Research Initiative from the US Department of Agriculture [2012-85117-19435]; US National Science Foundation [NSF-MCB-1339194]; EMBRAPA (Brazil) [02.11.08.006.00]; National High-Technology Research and Development Program (China) [2012AA02A701] FX We would like to thank G. Birdsong, V. Nwosu, J. Elder, D. Smyth, F. Luo, D. Hoisington, H. Shapiro, H. Valentine, R. Wilson and D. Ward for their support of and work for the Peanut Genome Initiative. We thank J.F.M. Valls, C. Simpson and G. Seijo for valuable discussions. Major financial contributors for this work were from Mars, US peanut sheller associations, the National Peanut Board and other industry groups. A full list can be downloaded at http://peanutbioscience.com/peanutgenomeproject.html. We also thank the generous support of the Agriculture and Food Research Initiative for grant 2012-85117-19435 from the US Department of Agriculture, the US National Science Foundation for grant NSF-MCB-1339194 to R.J.S., EMBRAPA (Brazil) for project 02.11.08.006.00 and the National High-Technology Research and Development Program (China) for grant 2012AA02A701. NR 82 TC 32 Z9 33 U1 18 U2 33 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD APR PY 2016 VL 48 IS 4 BP 438 EP + DI 10.1038/ng.3517 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA DH6NZ UT WOS:000372908800015 PM 26901068 ER PT J AU Cao, JJ Gregoire, BR AF Cao, Jay J. Gregoire, Brian R. TI A high-fat diet increases body weight and circulating estradiol concentrations but does not improve bone structural properties in ovariectomized mice SO NUTRITION RESEARCH LA English DT Article DE Bone structure; Obesity; High-fat diet; Ovariectomy; Mice ID AFRICAN-AMERICAN WOMEN; MASS INDEX; MINERAL DENSITY; ADIPOSE-TISSUE; HORMONE-LEVELS; OBESITY; ESTROGEN; MENOPAUSE; RATS; INTERLEUKIN-1 AB Bone health is influenced by body mass and estrogen. The objective of the study was to determine whether high-fat diet-induced obesity affects bone structure and alters markers of bone turnover in ovariectomized (OVX) mice. We hypothesized that a high-fat diet would increase body weight gain and serum estradiol levels in OVX mice but would not improve bone structural parameter in OVX mice. Thirty-five C57BL/6 mice were either sham operated or OVX at the age of 4 months and then fed either a normal-fat diet (10% energy as fat) or a high-fat diet (45% energy as fat with extra fat from lard) ad libitum for 11 weeks. Ovariectomy increased body weight, serum tartrate-resistant acid phosphatase concentration, and expression of cathepsin K in bone; decreased serum estradiol concentration; and induced significant bone loss manifested by decreased bone volume/total volume (BV/TV), connectivity density (Conn.D), trabecular number, and trabecular thickness with increased trabecular separation and structural model index (P < .01). The high-fat diet increased body weight (P < .01) in OVX mice and nonsignificantly decreased BV/TV (P = .08) and Conn.D (P = .10). Despite having similar serum estradiol concentrations and higher body weight, OVX mice consuming the high-fat diet had lower BV/TV, Conn.D, trabecular number, trabecular thickness, and higher structural model index and trabecular separation than did sham mice fed the normal-fat diet. These findings indicate that increased body weight and elevated serum estradiol concentration induced by a high-fat diet do not mitigate ovariectomy-induced bone loss in mice. Published by Elsevier Inc. C1 [Cao, Jay J.; Gregoire, Brian R.] USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58202 USA. RP Cao, JJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58202 USA. EM Jay.Cao@ars.usda.gov FU US Department of Agriculture's Agricultural Research Service program "Bone Metabolism in Obesity" [5450-51000-046-00D] FX We thank Forrest Nielson for reviewing and revising the manuscript and Steven Dufault and James Lindlauf for preparing experimental diets and conducting animal feeding experiment, laboratory biochemical, and mu CT analysis. This work was supported by the US Department of Agriculture's Agricultural Research Service program "Bone Metabolism in Obesity," Current Research Information System No. 5450-51000-046-00D. NR 42 TC 3 Z9 3 U1 3 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD APR PY 2016 VL 36 IS 4 BP 320 EP 327 DI 10.1016/j.nutres.2015.12.008 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI1HL UT WOS:000373247100004 PM 27001277 ER PT J AU Bell, DM Clark, JS AF Bell, David M. Clark, James S. TI Seed predation and climate impacts on reproductive variation in temperate forests of the southeastern USA SO OECOLOGIA LA English DT Article DE Masting; Reproductive ecology; Seed abortion; Seed predation; Trees ID ACORN PRODUCTION; RECRUITMENT LIMITATION; EVOLUTIONARY ECOLOGY; CHANGE VULNERABILITY; INDIVIDUAL VARIATION; HOST PLANTS; OAK FORESTS; OLD-GROWTH; MAST; DISPERSAL AB Climatic effects on tree recruitment will be determined by the interactive effects of fecundity and seed predation. Evaluating how insect and vertebrate seed predators mediate tree reproductive responses to climate depends on long-term studies of seed production, development, and predation. In this study, our objectives were to (1) assess the effects of interannual climate variation on seed abortion rates, (2) assess the impact of seed density on predation rates, and (3) examine the degree to which density-dependent seed predation would amplify or dampen interannual variation in fecundity associated with seed abortion. We used a 19-year study of seed abortion and pre-dispersal predation rates by insects and vertebrates (birds and rodents) for five temperate tree species across forest plots from the North Carolina Piedmont to the Southern Appalachian Mountains in the southeastern USA. We found that rates of seed abortion and predation increased reproductive variation for oaks (Quercus species). Probabilitey of seed abortion was greatest during years with cool, dry springs. Responses of seed predation on Quercus species to current year's seed density varid by species, but exhibited positive density-dependence to previous year's seed density consistent with numerical responses of seed predators. Seed abortion and predation rates for two drupe species responded little to variation in climate or seed density, respectively. Given that predation increased interannual variation in seed availability and the negative density-dependence to previous year's seed density, our results indicate that consistent numerical responses of oak seed predators may amplify interannual variation due to climate-mediated processes like seed abortion. C1 [Bell, David M.] USDA Forest Serv, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Clark, James S.] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Clark, James S.] Duke Univ, Dept Biol, Durham, NC USA. RP Bell, DM (reprint author), USDA Forest Serv, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM dmbell@fs.fed.us FU NSF [BSR-9444146, DEB 9453498, DEB-9632854, DEB-9981392, IDEA-0308498, DEB 0425465, SEII 0430693, DDDAS 0540347]; USDA Forest Service Pacific Northwest Research Station FX We would like to thank Norman Christensen, Fred Hain, Matthew Kwit, Megan Mobley, Ram Oren, Carl Salk, Anne Stein, Denis Valle, Kai Zhu, Ines Ibanez, and three anonymous reviewers for comments on the manuscript. The research was supported by NSF Grants BSR-9444146, DEB 9453498, DEB-9632854, DEB-9981392, IDEA-0308498, DEB 0425465, SEII 0430693 and DDDAS 0540347, as well as the USDA Forest Service Pacific Northwest Research Station. The experiments comply with the current laws of the United States of America in which the experiments were performed. NR 64 TC 1 Z9 1 U1 13 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2016 VL 180 IS 4 BP 1223 EP 1234 DI 10.1007/s00442-015-3537-6 PG 12 WC Ecology SC Environmental Sciences & Ecology GA DI0KV UT WOS:000373186100029 PM 26747267 ER PT J AU Dubey, JP Verma, SK Villena, I Aubert, D Geers, R Su, CL Lee, E Forde, MS Krecek, RC AF Dubey, Jitender P. Verma, Shiv Kumar Villena, Isabelle Aubert, Dominique Geers, Regine Su, Chunlei Lee, Elise Forde, Martin S. Krecek, Rosina C. TI Toxoplasmosis in the Caribbean islands: literature review, seroprevalence in pregnant women in ten countries, isolation of viable Toxoplasma gondii from dogs from St. Kitts, West Indies with report of new T-gondii genetic types SO PARASITOLOGY RESEARCH LA English DT Review DE Toxoplasma gondii; Human; Dog; Caribbean; Bioassay; Genotype ID TRINIDAD-AND-TOBAGO; SEROEPIDEMIOLOGICAL SURVEY; CONGENITAL TOXOPLASMOSIS; NEOSPORA-CANINUM; GRENADA; CATS; INFECTION; OOCYSTS; TRANSMISSION; GUADELOUPE AB Little is currently known of clinical toxoplasmosis in humans and animals in the Caribbean. We investigated the prevalence of IgG and IgM antibodies in 437 pregnant women from 10 English speaking Caribbean countries. Overall, antibodies (IgG) to Toxoplasma gondii (modified agglutination test, MAT, cut-off 1:6) were found in 174 (39.8 %) of 437 human sera; specifically 12 of 38 from Antigua-Barbuda, 26 of 52 from Belize, 9 of 50 from Bermuda, 29 of 49 from Dominica, 18 of 49 from Grenada, 16 of 47 from Jamaica, 5 of 15 from Montserrat, 8 of 44 from St. Kitts/Nevis, 24 of 45 from St. Lucia, and 27 of 50 from St. Vincent/Grenadines were seropositive. All IgG-positive sera were tested for IgM antibodies using the immunocapture method; all sera were negative for IgM antibodies. Additionally, tissues and sera of 45 dogs from St. Kitts were examined for T. gondii infection. Antibodies (IgG, MAT, 1:a parts per thousand yen25) were found in 19 (42.2 %) of 45 dogs. Muscle samples (tongue, leg) of 19 seropositive dogs were digested in pepsin, and homogenates were bioassayed in mice. Viable T. gondii were isolated from 6 dogs. T. gondii isolates were further propagated in cell culture. PCR-RFLP genotyping of cell culture derived tachyzoites using 10 genetic markers, SAG1, SAG2 (5' and 3' SAG2, and alt.SAG2) SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed that 4 isolates were ToxoDB PCR-RFLP genotype #2, and 2 were new genotypes #264 and #265. Review of 22 viable T. gondii isolates from chickens, dogs, and cats from Grenada and St. Kitts revealed that 1 isolate was type II, 13 were type III, and 8 were atypical. Thus, type III strains were predominant. Overall, the study revealed high prevalence of T. gondii in the Caribbean islands. C1 [Dubey, Jitender P.; Verma, Shiv Kumar] ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. [Villena, Isabelle; Aubert, Dominique; Geers, Regine] Univ Reims, Biol Ressource Ctr Toxoplasma, Natl Reference Ctr Toxoplasmosis, Lab Parasitol Mycol,EA 3800, 45 Rue Cognacq Jay, F-51092 Reims, France. [Villena, Isabelle; Aubert, Dominique; Geers, Regine] Hop Maison Blanche, 45 Rue Cognacq Jay, F-51092 Reims, France. [Su, Chunlei] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. [Lee, Elise; Krecek, Rosina C.] Ross Univ, Sch Vet Med, St Kitts, West Indies, Wales. [Forde, Martin S.] St Georges Univ, Sch Med, Dept Publ Hlth & Prevent Med, POB 7, St Georges, Grenada. [Krecek, Rosina C.] Texas A& M Univ One Hlth Initiat, Vet Pathobiol, Coll Sch Vet Med & Biomed Sci, College Stn, TX 77845 USA. [Krecek, Rosina C.] Univ Johannesburg, Dept Zool, Auckland Pk, South Africa. RP Dubey, JP (reprint author), ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov NR 37 TC 4 Z9 4 U1 3 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD APR PY 2016 VL 115 IS 4 BP 1627 EP 1634 DI 10.1007/s00436-015-4900-6 PG 8 WC Parasitology SC Parasitology GA DH6NL UT WOS:000372907300028 PM 26762861 ER PT J AU Zhou, DM Huang, XF Chaparro, JM Badri, DV Manter, DK Vivanco, JM Guo, JH AF Zhou, Dongmei Huang, Xing-Feng Chaparro, Jacqueline M. Badri, Dayakar V. Manter, Daniel K. Vivanco, Jorge M. Guo, Jianhua TI Root and bacterial secretions regulate the interaction between plants and PGPR leading to distinct plant growth promotion effects SO PLANT AND SOIL LA English DT Article DE Plant growth-promoting rhizobacteria; Root exudates; ABC transporters; Bacillus cereus; Bacterial secretions ID ATP-BINDING CASSETTE; CHROMATOGRAPHY MASS-SPECTROMETRY; PRECURSOR INDOLE-3-BUTYRIC ACID; PSEUDOMONAS-FLUORESCENS STRAIN; AZOSPIRILLUM-BRASILENSE SM; DEAMINASE GENE ACDS; RHIZOBACTERIA PGPR; BIOLOGICAL-CONTROL; BACILLUS-SUBTILIS; BIOFILM FORMATION AB Plant growth-promoting rhizobacteria (PGPR) have garnered interest in agriculture due to their ability to influence the growth and production of host plants. ATP-binding cassette (ABC) transporters play important roles in plant-microbe interactions by modulating plant root exudation. The present study aimed to provide a more precise understanding of the mechanism and specificity of the interaction between PGPR and host plants. In the present study, the effects of interactions between a PGPR strain, Bacillus cereus AR156, and Arabidopsis thaliana wild type (Col-0) or its ABC transporter mutants on plant growth have been studied. B. cereus AR156 promoted the shoot growth of Col-0 and Atabcg30 but repressed the growth of Atabcc5. Bacterial volatiles and secretion promoted the shoot growth of Col-0 and Atabcg30 but had no effect on Atabcc5. We also found that root exudates of Col-0 induced the expression of B. cereus AR156 genes related to siderophore and chitinase production; while root exudates of Atabcc5 inhibited the expression level of those genes. Further analysis of root exudates revealed that amino acids, organic acids, and sugars were significantly less abundant in Atabcc5 when compared to Col-0. Our findings highlight that both host plant and PGPR play active roles in the outcome of the plant-microbe interaction. C1 [Zhou, Dongmei; Guo, Jianhua] Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Jiangsu, Peoples R China. [Zhou, Dongmei; Guo, Jianhua] Nanjing Agr Univ, Engn Ctr Bioresource Pesticide Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. [Zhou, Dongmei; Guo, Jianhua] Nanjing Agr Univ, Natl & Local Joint Green Pesticide Formulat & App, Minist Educ, Nanjing 210095, Jiangsu, Peoples R China. [Zhou, Dongmei; Guo, Jianhua] Nanjing Agr Univ, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Jiangsu, Peoples R China. [Zhou, Dongmei; Huang, Xing-Feng; Chaparro, Jacqueline M.; Badri, Dayakar V.; Vivanco, Jorge M.] Colorado State Univ, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA. [Huang, Xing-Feng] Colorado State Univ, Dept Chem & Biol Engn, Ft Collins, CO 80523 USA. [Manter, Daniel K.] USDA ARS, Soil Plant Nutr Res Unit, Ft Collins, CO 80526 USA. RP Guo, JH (reprint author), Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing 210095, Jiangsu, Peoples R China. EM jhguo@njau.edu.cn FU National Natural Science Foundation of China [31471812, 31171809]; China Scholarship Council [201206850028]; Colorado State University Agricultural Experiment Station FX We thank members of Professors Vivanco's and Guo's groups for technical assistance and helpful discussions. We especially thank Prof. Congfeng Song (Nanjing Agricultural University, China) and Dr. Azeddine Driouich (Universite' de Rouen, France) for helpful suggestions. This work was supported by National Natural Science Foundation of China (No. 31471812 and No. 31171809) to JG, China Scholarship Council (No. 201206850028) to DZ, and by Colorado State University Agricultural Experiment Station. NR 99 TC 0 Z9 0 U1 23 U2 60 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD APR PY 2016 VL 401 IS 1-2 BP 259 EP 272 DI 10.1007/s11104-015-2743-7 PG 14 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA DH7CC UT WOS:000372947800019 ER PT J AU Olanrewaju, HA Miller, WW Maslin, WR Collier, SD Purswell, JL Branton, SL AF Olanrewaju, H. A. Miller, W. W. Maslin, W. R. Collier, S. D. Purswell, J. L. Branton, S. L. TI Effects of light sources and intensity on broilers grown to heavy weights. Part 1: Growth performance, carcass characteristics, and welfare indices SO POULTRY SCIENCE LA English DT Article DE light sources; light intensity; growth performance; broilers; welfare ID MONOCHROMATIC LIGHT; DOMESTICUS; MELATONIN; IMMUNE; COLOR; GREEN AB This study investigated the effects of light sources and light intensity on growth performance, carcass characteristics, and welfare indices of heavy broilers (>3.0 kg) in 4 trials with 2 replications per trial. A total of 960 one-day-old Ross x Ross 708 chicks (30 males/30females/room) were randomly distributed into 16 environmentally controlled rooms at 50% RH. A 4 x 2 factorial treatment structure evaluated 4 light sources (incandescent [ICD, standard], compact fluorescent [CFL], neutral light emitting diode [Neutral-LED], and cool poultry specific filtered LED [Cool-PSF-LED]) and 2 levels (5 lx, 20 lx) of light intensities. Each of the 4 light sources was paired with one of the 2 light intensities. Birds were fed the same diet with a 4-phase feeding program (starter, grower, finisher, and withdrawal). Feed and water were provided ad libitum. Birds and feed were weighed on one, 14, 21, 28, 42, and 56 d of age for growth performance. All mortality was recorded daily and feed conversion was adjusted for mortality. Immune response was determined on d 28 to d 35, whereas other welfare indices were performed on d 42 and 49, respectively. At d 56 of each trial, 20 (10 males and 10 females) birds from each room were processed to determine weights and yields. The BW, BW gain, live weight, and carcass weights of birds reared under Cool-PSF-LED were different (P <= 0.05) in comparison to birds reared under ICD. However, FI, FCR, and mortality were not affected by treatments. There was no effect of treatments on fat or breast and tender weights and yields. In addition, there was no effect of treatments on ocular development, immune response, and other welfare indices, suggesting that the light sources evaluated did not compromise welfare of heavy broilers. It was concluded that the 3 light sources evaluated in this study may be suitable for replacement of ICD light source in poultry facilities to reduce energy cost and optimize production efficiency. C1 [Olanrewaju, H. A.; Collier, S. D.; Purswell, J. L.; Branton, S. L.] Mississippi State Univ, ARS, USDA, Poultry Res Unit, POB 5367, Starkville, MS 39762 USA. [Miller, W. W.] Adv Anim Eye Care, 1067 Old West Point Rd, Starkville, MS 39759 USA. [Maslin, W. R.] Mississippi State Univ, Coll Vet Med, Wise Ctr, Starkville, MS 39762 USA. RP Olanrewaju, HA (reprint author), Mississippi State Univ, ARS, USDA, Poultry Res Unit, POB 5367, Starkville, MS 39762 USA. EM Hammed.Olanrewaju@ars.usda.gov NR 42 TC 0 Z9 0 U1 12 U2 29 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD APR PY 2016 VL 95 IS 4 BP 727 EP 735 DI 10.3382/ps/pev360 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DH9WZ UT WOS:000373148000001 PM 26908888 ER PT J AU Bakst, MR Bauchan, G AF Bakst, M. R. Bauchan, G. TI Lectin staining of the uterovaginal junction and sperm-storage tubule epithelia in broiler hens SO POULTRY SCIENCE LA English DT Article DE oviduct; sperm storage; broiler breeder; glycoconjugates ID FOWL GALLUS-DOMESTICUS; OVIDUCT; SPERMATOZOA; RELEASE; MECHANISM; GLANDS AB Mammalian sperm bind to terminal carbohydrates associated with glycoconjugates on the apical surface of oviduct epithelial cells in the caudal region of the oviduct and undergo cellular and molecular modifications associated with capacitation prior to ovulation. In contrast, chicken sperm are stored for up to 23 d in sperm-storage tubules (SST) localized in the uterovaginal junction (UVJ). Little is known of the cellular and molecular mechanisms that regulate sperm storage in and release from the SST. The purpose of this study was to identify glycoconjugates associated with the SST epithelial cell surface using lectins. Virgin hens and hens of higher and lower fertility in egg production for 6 to 16 wk were used in this study. Sections of UVJ mucosa containing SST were stained with fluorescent conjugated lectins and examined by confocal microscopy. Carbohydrate moieties associated with the UVJ and SST epithelia differed in their lectin binding patterns. No differences in the lectin binding patterns within the 2 epithelia were discernible between the virgin and younger and older hens. Minor differences were observed between the higher and lower fertility hens. Only lectins specific for galactose and N-acetylgalactosamine moieties were localized to the luminal surface of the SST. While resident sperm may be closely apposed to the SST epithelial cell apical microvilli, it is unlikely that sperm binding to the microvilli via terminal carbohydrates associated with glycoconjugates is a requisite for prolonged storage. However, the possibility of SST epithelial cell communication with resident sperm via shedding microvillous vesicles characterized by surface glycoconjugates with terminal galactose and N-acetylgalactosamine moieties is currently being investigated. C1 [Bakst, M. R.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Bauchan, G.] ARS, Electron & Confocal Microscopy Unit, Beltsville Area, USDA, Beltsville, MD 20705 USA. RP Bakst, MR (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. EM murray.bakst@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD APR PY 2016 VL 95 IS 4 BP 948 EP 955 DI 10.3382/ps/pev440 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DH9WZ UT WOS:000373148000026 PM 26769269 ER PT J AU Heisler, GM Ellis, A Nowak, DJ Yesilonis, I AF Heisler, Gordon M. Ellis, Alexis Nowak, David J. Yesilonis, Ian TI Modeling and imaging land-cover influences on air temperature in and near Baltimore, MD SO THEORETICAL AND APPLIED CLIMATOLOGY LA English DT Article ID URBAN HEAT-ISLAND; CLIMATE; VEGETATION; ARIZONA; IMPACTS; PHOENIX; SIMULATION; QUALITY; ALBEDO; CITIES AB Over the course of 1681 hours between May 5 and September 30, 2006, air temperatures measured at the 1.5-m height at seven sites in and near the city of Baltimore, MD were used to empirically model Delta (R-p) , the difference in air temperature between a site in downtown Baltimore and the six other sites. Variables in the prediction equation included difference between the downtown reference and each of the other sites in upwind tree cover and impervious cover as obtained from 10-m resolution geographic information system (GIS) data. Other predictor variables included an index of atmospheric stability, topographic indices, wind speed, vapor pressure deficit, and antecedent precipitation. The model was used to map predicted hourly Delta (R-p) across the Baltimore region based on hourly weather data from the airport. Despite the numerous sources of variability in the regression modeling, the method produced reasonable map patterns of Delta (R-p) that, except for some areas evidently affected by sea breeze from the Chesapeake, closely matched results of mesoscale modeling. Potential applications include predictions of the effect of changing tree cover on air temperature in the area. C1 [Heisler, Gordon M.; Nowak, David J.] SUNY Coll Environm Sci & Forestry, USDA, Forest Serv, Northern Res Stn, 5 Moon Lib,1 Forestry Dr, Syracuse, NY 13210 USA. [Ellis, Alexis] USDA, Forest Serv, Davey Inst, 5 Moon Lib,1 Forestry Dr, Syracuse, NY 13210 USA. [Yesilonis, Ian] Baltimore Ecosyst Study, USDA, Forest Serv, No Res Stn, 5523 Res Pk,Suite 350, Baltimore, MD 21228 USA. RP Heisler, GM (reprint author), SUNY Coll Environm Sci & Forestry, USDA, Forest Serv, Northern Res Stn, 5 Moon Lib,1 Forestry Dr, Syracuse, NY 13210 USA. EM gmheisler@fs.fed.us FU National Science Foundation [DEB 0423476] FX Many individuals contributed in various ways to this study including Gregory Bacon, Kenneth Belt, Michelle Bunny, Richard Grant, Eric Greenfield, Sue Grimmond, Peter Groffman, Morgan Grove, John Hom, Karla Hyde, Andrew Lee, Jarlath O'Neil-Dunne, Richard Pouyat, Emma Powell, Hang Ryeol Na, Baohua Tao, Jeffrey Walton, and Yingjie Wang. John Stanovick, statistician with the USDA Forest Service, made numerous suggestions on the statistical analyses. This paper benefited from reviews of an earlier version by Anthony J. Brazel and Winston Chow. The Baltimore Ecosystem Study LTER, operating with contributions from the National Science Foundation grant DEB 0423476, provided some of the technical support staff and instrumentation. Two anonymous reviewers provided unusually helpful and detailed suggestions for improving the manuscript. NR 67 TC 0 Z9 0 U1 8 U2 13 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0177-798X EI 1434-4483 J9 THEOR APPL CLIMATOL JI Theor. Appl. Climatol. PD APR PY 2016 VL 124 IS 1-2 BP 497 EP 515 DI 10.1007/s00704-015-1416-z PG 19 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DH9VH UT WOS:000373143600038 ER PT J AU Qi, L Foley, M Cai, X Gulya, T AF Qi, L. L. Foley, M. E. Cai, X. W. Gulya, T. J. TI Genetics and mapping of a novel downy mildew resistance gene, Pl (18) , introgressed from wild Helianthus argophyllus into cultivated sunflower (Helianthus annuus L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MARKER-ASSISTED SELECTION; EASTERN NORTH-AMERICA; PCR-BASED MARKERS; PLASMOPARA-HALSTEDII; GERMPLASM LINES; MALE-STERILITY; PL5/PL8 LOCUS; RICH REGIONS; GENOME; IDENTIFICATION AB A novel downy mildew resistance gene, Pl (18) , was introgressed from wild Helianthus argophyllus into cultivated sunflower and genetically mapped to linkage group 2 of the sunflower genome. The new germplasm, HA-DM1, carrying Pl (18) has been released to the public. Sunflower downy mildew (DM) is considered to be the most destructive foliar disease that has spread to every major sunflower-growing country of the world, except Australia. A new dominant downy mildew resistance gene (Pl (18) ) transferred from wild Helianthus argophyllus (PI 494573) into cultivated sunflower was mapped to linkage group (LG) 2 of the sunflower genome using bulked segregant analysis with 869 simple sequence repeat (SSR) markers. Phenotyping 142 BC1F2:3 families derived from the cross of HA 89 and H. argophyllus confirmed the single gene inheritance of resistance. Since no other Pl gene has been mapped to LG2, this gene was novel and designated as Pl (18). SSR markers CRT214 and ORS203 flanked Pl (18) at a genetic distance of 1.1 and 0.4 cM, respectively. Forty-six single nucleotide polymorphism (SNP) markers that cover the Pl (18) region were surveyed for saturation mapping of the region. Six co-segregating SNP markers were 1.2 cM distal to Pl (18) , and another four co-segregating SNP markers were 0.9 cM proximal to Pl (18) . The new BC2F4-derived germplasm, HA-DM1, carrying Pl (18) has been released to the public. This new line is highly resistant to all Plasmopara halstedii races identified in the USA providing breeders with an effective new source of resistance against downy mildew in sunflower. The molecular markers that were developed will be especially useful in marker-assisted selection and pyramiding of Pl resistance genes because of their close proximity to the gene and the availability of high-throughput SNP detection assays. C1 [Qi, L. L.; Foley, M. E.; Gulya, T. J.] USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. [Cai, X. W.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Qi, L (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU National Sunflower Association Agreement [12-D02]; USDA-ARS CRIS Project [5442-21000-039-00D]; USDA-AMS Specialty Crop Block Grant Program [12-25-B-1689] FX The authors would like to thank Dr. Loren Rieseberg for providing access to the Sunflower Genome Data Repository. We also thank Drs. Gerald Seiler and Steven Xu for critical review of the manuscript, and Angelia Hogness and Cheryl Huckle for technical assistance. This project was supported by the National Sunflower Association Agreement 12-D02, the USDA-ARS CRIS Project No. 5442-21000-039-00D, and the USDA-AMS Specialty Crop Block Grant Program 12-25-B-1689. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 77 TC 5 Z9 5 U1 2 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2016 VL 129 IS 4 BP 741 EP 752 DI 10.1007/s00122-015-2662-2 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DH6MC UT WOS:000372903700007 PM 26747047 ER PT J AU Li, K Hegarty, J Zhang, CZ Wan, AM Wu, JJ Guedira, G Chen, XM Munoz-Amatriain, M Fu, DL Dubcovsky, J AF Li, Kun Hegarty, Joshua Zhang, Chaozhong Wan, Anmin Wu, Jiajie Guedira, Gina Brown Chen, Xianming Munoz-Amatriain, Maria Fu, Daolin Dubcovsky, Jorge TI Fine mapping of barley locus Rps6 conferring resistance to wheat stripe rust SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; YELLOW PIGMENT CONTENT; PUCCINIA-STRIIFORMIS; NONHOST RESISTANCE; UNITED-STATES; HORDEUM-VULGARE; VIRULENCE; GENE; GENOME; ARABIDOPSIS AB Barley resistance to wheat stripe rust has remained effective for a long time and, therefore, the genes underlying this resistance can be a valuable tool to engineer durable resistance in wheat. Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major disease of wheat that is causing large economic losses in many wheat-growing regions of the world. Deployment of Pst resistance genes has been an effective strategy for controlling this pathogen, but many of these genes have been defeated by new Pst races. In contrast, genes providing resistance to this wheat pathogen in other grass species (nonhost resistance) have been more durable. Barley varieties (Hordeum vulgare ssp. vulgare) are predominately immune to wheat Pst, but we identified three accessions of wild barley (Hordeum vulgare ssp. spontaneum) that are susceptible to Pst. Using these accessions, we mapped a barley locus conferring resistance to Pst on the distal region of chromosome arm 7HL and designated it as Rps6. The detection of the same locus in the cultivated barley 'Tamalpais' and in the Chinese barley 'Y12' by an allelism test suggests that Rps6 may be a frequent component of barley intermediate host resistance to Pst. Using a high-density mapping population (> 10,000 gametes) we precisely mapped Rps6 within a 0.14 cM region (similar to 500 kb contig) that is colinear to regions in Brachypodium (< 94 kb) and rice (< 9 kb). Since no strong candidate gene was identified in these colinear regions, a dedicated positional cloning effort in barley will be required to identify Rps6. The identification of this and other barley genes conferring resistance to Pst can contribute to our understanding of the mechanisms for durable resistance against this devastating wheat pathogen. C1 [Li, Kun; Zhang, Chaozhong; Wu, Jiajie; Fu, Daolin] Shandong Agr Univ, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China. [Li, Kun; Hegarty, Joshua; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Wan, Anmin; Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Guedira, Gina Brown] N Carolina State Univ, Dept Crop Sci, Plant Sci Res Unit, USDA ARS, Raleigh, NC 27695 USA. [Chen, Xianming] USDA ARS, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Munoz-Amatriain, Maria] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. RP Fu, DL (reprint author), Shandong Agr Univ, State Key Lab Crop Biol, Shandong Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China.; Dubcovsky, J (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA.; Dubcovsky, J (reprint author), Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. EM dlfu@sdau.edu.cn; jdubcovsky@ucdavis.edu RI Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU National Natural Science Foundation of China [31110103917]; National Basic Research Program of China [2013CB127700, 2011CB100700]; Tai'shan Scholar Program of Shandong Province, China; Cooperative Innovation Center of Efficient Production with High Annual Yield of Wheat and Corn, Shandong Province, China; NRI from the USDA National Institute of Food and Agriculture [2011-68002-30029]; Borlaug Global Rust Initiative; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation [GBMF3031] FX We thank Qiang Liu and Bo Lv for maintaining the barley populations and Qunqun Hao for help with the expression experiments. We thank Dr. Xianchun Xia for providing the Chinese barley line 'Y12', Dr. J. Dvorak and Dr. M.-C. Luo for his help with the A. tauschii sequences, Dr. T. Close (UC Riverside), and Dr. N. Stein and Martin Mascher (IPK, Getersleben) and the International Barley Genome Sequencing Consortium for their assistance with the barley genomic sequences. We thank Dr. M Moscou for his valuable suggestions during the preparation of this manuscript. Dr. Fu acknowledges support from the National Natural Science Foundation of China (31110103917), the National Basic Research Program of China (2013CB127700 and 2011CB100700), the Tai'shan Scholar Program of Shandong Province, China, and the Cooperative Innovation Center of Efficient Production with High Annual Yield of Wheat and Corn, Shandong Province, China. Dr. Dubcovsky acknowledges support from the NRI grant 2011-68002-30029 (TCAP) from the USDA National Institute of Food and Agriculture, by the Borlaug Global Rust Initiative, and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation Grant GBMF3031. NR 45 TC 1 Z9 1 U1 7 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2016 VL 129 IS 4 BP 845 EP 859 DI 10.1007/s00122-015-2663-1 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DH6MC UT WOS:000372903700014 PM 26875072 ER PT J AU Hergert, GW Margheim, JF Pavlista, AD Martin, DL Supalla, RJ Isbell, TA AF Hergert, G. W. Margheim, J. F. Pavlista, A. D. Martin, D. L. Supalla, R. J. Isbell, T. A. TI Yield, irrigation response, and water productivity of deficit to fully irrigated spring canola SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Biodiesel; Oilseed crops; High Plains aquifer; Ground water decline ID LIMITED IRRIGATION; OILSEED CROPS; CROPPING SYSTEMS; GREAT-PLAINS; MUSTARD; CLIMATE; GROWTH; OIL AB Canola (Brassica napus) is an oil-seed crop that is adapted to the northern High Plains of the USA and is considered a viable rotational and biofuel crop. However, decreased ground water allocations have necessitated determining the impact of limited irrigation on canola productivity. The objectives of this research were to determine the effects of a range of irrigation from none to fully irrigated on yield, oil content, soil water changes and water productivity of spring canola. The study was conducted for four growing seasons at three locations in western Nebraska. Two sites had sandy soils whereas the other was a silt loam. Glyphosate tolerant canola was planted late March to early April. Cumulative irrigation treatments were 0, 100, 200, and 300 mm of water with the highest rate adjusted to be non-ET limiting. Canola extracted soil water from depths greater than 1.2 m in both fine textured and sandy soils making it a good alternative for deficit irrigation. Canola responded well to irrigation during dry years but showed little response in above average precipitation years. A water use efficiency of 7.6 kg mm(-1) with a threshold of 123 mm was observed. Canola seed yield ranged from 440 to 3280 kg ha(-1) with 165 and 582 mm of cumulative ET. During drier years, canola exhibited peak values in water use at 8-9 weeks after planting. Deficit irrigation reduced ET and yield and hastened maturity during drier years. Oil content was increased by irrigation during, drier years with no effect shown when precipitation was above average. Oil content ranged from 30 to 50% depending on year and irrigation level. The water response provided benefits of not only higher yield, but also higher oil content which makes deficit irrigated canola an attractive alternative production and biofuel crop for this region. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hergert, G. W.; Margheim, J. F.; Pavlista, A. D.] Univ Nebraska Lincoln, PREC, Scottsbluff, NE 69361 USA. [Martin, D. L.; Supalla, R. J.] Univ Nebraska, Lincoln, NE 68583 USA. [Isbell, T. A.] USDA ARS, Peoria, IL 61604 USA. RP Hergert, GW (reprint author), Univ Nebraska Lincoln, PREC, Scottsbluff, NE 69361 USA. EM ghergert1@unl.edu FU USDA Risk Management Agency FX Funding for this project was provided by the USDA Risk Management Agency under several contracts. Without their support we could not have completed this research. NR 34 TC 2 Z9 2 U1 5 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD APR PY 2016 VL 168 BP 96 EP 103 DI 10.1016/j.agwat.2016.02.003 PG 8 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DH4MF UT WOS:000372759400010 ER PT J AU Tomer, MD Moorman, TB Kovar, JL Cole, KJ Nichols, DJ AF Tomer, M. D. Moorman, T. B. Kovar, J. L. Cole, K. J. Nichols, D. J. TI Eleven years of runoff and phosphorus losses from two fields with and without manure application, Iowa, USA SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Runoff; Phosphorus; Edge-of-field monitoring; Manure ID SWINE MANURE; COVER CROPS; NITRATE; WATERSHEDS; NUTRIENT; SOIL; REDUCTIONS; NITROGEN; TILLAGE; PRAIRIE AB Monitoring runoff at field edges can show how cropping systems and conservation practices affect runoff hydrology and water quality. Multi-year records are needed to characterize these effects, because of the variable, ephemeral nature of rainfall-runoff events. This study compared runoff and phosphorus (P) losses from two fields in central Iowa from 2000 through 2010. Both fields were managed in the same three-year, corn (Zea mays L.)-corn-soybean (Glycine max (L.) Merr.) rotation, but one field received applications of swine manure for each year of corn. Results comprised 116 events at the manured site and 94 events at the non-manured site, with 74 events common to both locations. Rainfall-runoff relationships for the two fields were similar; annual runoff averaged 54 mm from the non-manured field and 47 mm from the manured field. Large storms (>60 mm rainfall) comprised about 10% of the runoff events in both watersheds, producing 12-16% of the total P loads. Moderate storms (30-60 mm rainfall) generated most (65-70%) of the P load from both watersheds. Losses of P averaged 1.8 kg P ha(-1) year(-1) from the manured watershed and 1.05 kg P ha(-1) year(-1) from the non-manured watershed. Relationships between runoff volumes and P-loads differed between the two watersheds (p<0.05). Results highlight the challenge of maintaining adequate soil P levels while minimizing runoff P losses under a corn-soybean rotation, but indicate conservation practices that can limit runoff from storms of 30-60 mm of rainfall can help producers meet that challenge. Published by Elsevier B.V. C1 [Tomer, M. D.; Moorman, T. B.; Kovar, J. L.; Cole, K. J.; Nichols, D. J.] USDA ARS, Natl Lab Agr & Environm, 1015N Univ Blvd, Ames, IA 50011 USA. RP Tomer, MD (reprint author), USDA ARS, Natl Lab Agr & Environm, 1015N Univ Blvd, Ames, IA 50011 USA. EM mark.tomer@ars.usda.gov NR 33 TC 1 Z9 1 U1 14 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD APR PY 2016 VL 168 BP 104 EP 111 DI 10.1016/j.agwat.2016.01.011 PG 8 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DH4MF UT WOS:000372759400011 ER PT J AU Parajuli, PB Jayakody, P Sassenrath, GF Ouyang, Y AF Parajuli, P. B. Jayakody, P. Sassenrath, G. F. Ouyang, Y. TI Assessing the impacts of climate change and tillage practices on stream flow, crop and sediment yields from the Mississippi River Basin SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Climate change; Modeling; Tillage practices; Stream flow; Crop yield ID CONTERMINOUS UNITED-STATES; GREAT-BARRIER-REEF; CHANGE SCENARIOS; WATER-QUALITY; AGRICULTURAL PRODUCTIVITY; INTEGRATED ASSESSMENT; MODELING APPROACH; SWAT MODEL; PRECIPITATION; VARIABILITY AB This study evaluated climate change impacts on stream flow, crop and sediment yields from three different tillage systems (conventional, reduced 1 close to conservation, and reduced 2 close to no-till), in the Big Sunflower River Watershed (BSRW) in Mississippi. The Soil and Water Assessment Tool (SWAT) model was applied to the BSRW using observed stream flow and crop yields data. The model was calibrated and validated successfully using monthly stream flow data (2001-2011). The model performances showed the regression coefficient (R-2) from 0.72 to 0.82 and Nash-Sutcliffe efficiency index (NSE) from 0.70 to 0.81 for streamflow; R-2 from 0.40 to 0.50 and NSE from 0.72 to 0.86 for corn yields; and R-2 from 0.43 to 0.59 and NSE from 0.48 to 0.57 for soybeans yields. The Long Ashton Research Station Weather Generator (LARS-WG), was used to generate future climate scenarios. The SRES (Special Report on Emissions Scenarios) A1B, A2, and B1 climate change scenarios of the Intergovernmental Panel on Climate Change (IPCC) were simulated for the mid (2046-2065) and late (2080-2099) century. Model outputs showed slight differences among tillage practices for corn and soybean yields. However, model simulated sediment yield results indicated a large difference among the tillage practices from the corn and soybean crop fields. The simulated future average maximum temperature showed as high as. 4.8 degrees C increase in the BSRW. Monthly precipitation patterns will remain un-changed based on simulated future climate scenarios except for an increase in the frequency of extreme rainfall events occurring in the watershed. On average, the effect of climate change and tillage practice together did not show notable changes to the future crop yields. The reduced tillage 2 practices showed the highest responses of erosion control to climate change followed by the reduced tillage 1 and conventional tillage in this study. (C) 2016 Elsevier B.V. All rights reserved. C1 [Parajuli, P. B.; Jayakody, P.] Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. [Sassenrath, G. F.] Kansas State Univ, Parsons, KS USA. [Ouyang, Y.] US Forest Serv, USDA, Mississippi State, MS USA. RP Parajuli, PB (reprint author), Mississippi State Univ, Dept Agr & Biol Engn, Mississippi State, MS 39762 USA. EM pparajuli@abe.msstate.edu FU AFRI Competitive Grant from the USDA/NIFA [67020-21407]; Special Research Initiatives of the Mississippi Agricultural and Forestry Experiment Station at Mississippi State University; Yazoo Mississippi Delta Joint Water Management District; Stoneville and Clarksdale Agricultural Experiment Stations FX We would like to acknowledge the partial financial support of AFRI Competitive Grant (Project #67020-21407) from the USDA/NIFA; and Special Research Initiatives of the Mississippi Agricultural and Forestry Experiment Station at Mississippi State University. We would like to acknowledge the support of Yazoo Mississippi Delta Joint Water Management District; Mr. Kenneth Hood; Stoneville and Clarksdale Agricultural Experiment Stations; and all our collaborators. This manuscript is contribution number 15-172-J from the Kansas Agricultural Experiment Station. NR 80 TC 1 Z9 1 U1 18 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD APR PY 2016 VL 168 BP 112 EP 124 DI 10.1016/j.agwat.2016.02.005 PG 13 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DH4MF UT WOS:000372759400012 ER PT J AU McKinnon, RA Siddiqi, SM Chaloupka, FJ Mancino, L Prasad, K AF McKinnon, Robin A. Siddiqi, Sameer M. Chaloupka, Frank J. Mancino, Lisa Prasad, Kislaya TI Obesity-Related Policy/Environmental Interventions A Systematic Review of Economic Analyses SO AMERICAN JOURNAL OF PREVENTIVE MEDICINE LA English DT Review ID NUTRITION EDUCATION-PROGRAM; BODY-MASS INDEX; COST-EFFECTIVENESS; PREVENTION PROGRAM; EXPANDED FOOD; NATURAL EXPERIMENT; PHYSICAL-ACTIVITY; UNITED-STATES; CARE COSTS; HEALTH AB Context: Policy and environmental changes to support and encourage individual-level nutrition and physical activity behavior are underway in many parts of the U.S. and around the world at national, state, and local levels. Yet, to the authors' knowledge, no summary of the cost-benefit or cost-effectiveness studies of obesity-related policy/environmental interventions exists. Evidence acquisition: The PRISMA (Preferred Reporting Items for Systematic Reviews and Meta Analyses) statement guidelines were followed to identify, screen, and describe the protocols used in this systematic review. In 2014, a unique search was conducted of titles and abstracts in MEDLINE, EconLit, SCOPUS, and Web of Science databases that were published from January 2002 through January 2014 in English-language, peer-reviewed journals. The search terms described obesity, physical activity, and diet in combination with economic evaluation. Evidence synthesis: In 2014 and 2015, the results were analyzed. A total of 27 studies met the inclusion criteria, of which 26 described separate interventions. Of the 27 included studies, eight focused on the community and built environment, seven assessed nutrition-related changes, nine reported on the school environment, and three evaluated social marketing and media interventions. The vast majority of included studies reported beneficial economic outcomes of the interventions. Conclusions: Given the large and growing literature on the health and behavioral outcomes of policy and environmental interventions, the relatively low number of located cost-beneft and cost-effectiveness economic assessments appears to indicate a prime opportunity for the research community to address. Published by Elsevier Inc. on behalf of American Journal of Preventive Medicine C1 [McKinnon, Robin A.] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA. [McKinnon, Robin A.] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. [Siddiqi, Sameer M.] Johns Hopkins Univ, Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Chaloupka, Frank J.] Univ Illinois, Inst Hlth Res & Policy, Ctr Hlth Policy, Chicago, IL USA. [Mancino, Lisa] Econ Res Serv, USDA, Washington, DC USA. [Prasad, Kislaya] Univ Maryland, Robert H Smith Sch Business, College Pk, MD 20742 USA. RP McKinnon, RA (reprint author), US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. EM robin.mckinnon@fda.hhs.gov FU Robert Wood Johnson Foundation FX The authors are grateful to Penny Randall-Levy for expert referencing support. Funding for Frank J. Chaloupka's time was supported by the Robert Wood Johnson Foundation's grant to the University of Illinois at Chicago for the Bridging the Gap program. NR 56 TC 0 Z9 0 U1 13 U2 33 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0749-3797 EI 1873-2607 J9 AM J PREV MED JI Am. J. Prev. Med. PD APR PY 2016 VL 50 IS 4 BP 543 EP 549 DI 10.1016/j.amepre.2015.10.021 PG 7 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA DH1RP UT WOS:000372562900014 PM 26707464 ER PT J AU Park, M Deck, J Foley, SL Nayak, R Songer, JG Seibel, JR Khan, SA Rooney, AP Hecht, DW Rafii, F AF Park, Miseon Deck, Joanna Foley, Steven L. Nayak, Rajesh Songer, J. Glenn Seibel, Janice R. Khan, Saeed A. Rooney, Alejandro P. Hecht, David W. Rafii, Fatemeh TI Diversity of Clostridium perfringens isolates from various sources and prevalence of conjugative plasmids SO ANAEROBE LA English DT Article DE Clostridium perfringens; Plasmid; Epidemiology; Diversity; PFGE ID TOXIN PLASMIDS; GEL-ELECTROPHORESIS; GENETIC DIVERSITY; STRAINS AB Clostridium perfringens is an important pathogen, causing food poisoning and other mild to severe infections in humans and animals. Some strains of C. perfringens contain conjugative plasmids, which may carry antimicrobial resistance and toxin genes. We studied genomic and plasmid diversity of 145 C. perfringens type A strains isolated from soils, foods, chickens, clinical samples, and domestic animals (porcine, bovine and canine), from different geographic areas in the United States between 1994 and 2006, using multiple-locus variable-number tandem repeat analysis (MLVA) and/or pulsed-field gel electrophoresis (PFGE). MLVA detected the genetic diversity in a majority of the isolates. PFGE, using SmaI and KspI, confirmed the MLVA results but also detected differences among the strains that could not be differentiated by MLVA. All of the PFGE profiles of the strains were different, except for a few of the epidemiologically related strains, which were identical. The PFGE profiles of strains isolated from the same domestic animal species were clustered more closely with each other than with other strains. However, a variety of C. perfringens strains with distinct genetic backgrounds were found among the clinical isolates. Variation was also observed in the size and number of plasmids in the strains. Primers for the internal fragment of a conjugative tcpH gene of C. perfringens plasmid pCPF4969 amplified identical size fragments from a majority of strains tested; and this gene hybridized to the various-sized plasmids of these strains. The sequences of the PCR-amplified tcpH genes from 12 strains showed diversity among the tcpH genes. Regardless of the sources of the isolates, the genetic diversity of C. perfringens extended to the plasmids carrying conjugative genes. Published by Elsevier Ltd. C1 [Park, Miseon; Deck, Joanna; Foley, Steven L.; Nayak, Rajesh; Khan, Saeed A.; Rafii, Fatemeh] US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. [Songer, J. Glenn] Univ Arizona, Tucson, AZ 85721 USA. [Seibel, Janice R.] Iowa State Univ, Ames, IA 50011 USA. [Rooney, Alejandro P.] ARS, Crop Protect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Hecht, David W.] Loyola Univ, Med Ctr, Div Infect Dis, Maywood, IL 60126 USA. RP Rafii, F (reprint author), US FDA, Div Microbiol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. EM fatemeh.rafii@fda.hhs.gov NR 31 TC 1 Z9 2 U1 1 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1075-9964 EI 1095-8274 J9 ANAEROBE JI Anaerobe PD APR PY 2016 VL 38 BP 25 EP 35 DI 10.1016/j.anaerobe.2015.11.003 PG 11 WC Microbiology SC Microbiology GA DG9FG UT WOS:000372387600005 PM 26608548 ER PT J AU Freking, BA Lents, CA Vallet, JL AF Freking, B. A. Lents, C. A. Vallet, J. L. TI Selection for uterine capacity improves lifetime productivity of sows SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Swine; Parity; Uterine capacity; Lifetime production ID OVULATION RATE; LITTER SIZE; SWINE; TRAITS; GROWTH; LINES AB Selection for 11 generations for uterine capacity (UC) increased litter size in gilts by 1.6 more fully formed pigs at birth compared to an unselected control line (CO) despite averaging one less ovulation. The objective of the present study was to quantify line by parity interactions and characterize litter performance traits of sows in each line at later parities. Gilts farrowed in contemporary groups of 19 litters and were maintained through four parities if successfully mated in that contemporary group. A total of 243 litters and 2639 piglets were analyzed. Fixed effects of farrowing group, line, parity (1-4), and two-way interactions involving line were fitted. Sire (n = 57) of the sow within farrowing group and line was fitted as a random effect. No significant line by parity interactions were observed. Parity effects were detected (P < 0.01) for individual piglet birth weight, pre-weaning gain, and weaning weight. Parity effects were also detected (P < 0.05) for total number born, average and total litter birth weight, and average and total litter weaning weight. Selection line differences for litter traits were detected (P < 0.05) for number stillborn piglets and approached significance (P = 0.06) for number of piglets weaned. Retention of sows in the herd was greater (P < 0.05) with an average of 2.33 parities for the UC line females compared to 1.87 parities for the CO line. This resulted in favorable cumulative lifetime productivity of the UC line for total number of piglets born, number of piglets born alive, litter birth weight, number of piglets weaned and litter weaning weight. (C) 2016 Published by Elsevier B.V. C1 [Freking, B. A.; Lents, C. A.; Vallet, J. L.] ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. RP Freking, BA (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM brad.freking@ars.usda.gov NR 10 TC 1 Z9 1 U1 9 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 EI 1873-2232 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD APR PY 2016 VL 167 BP 16 EP 21 DI 10.1016/j.anireprosci.2016.01.018 PG 6 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA DH4NK UT WOS:000372762500003 PM 26869209 ER PT J AU Bell, JG Strachan, F Roy, WJ Matthew, C McDonald, P Barrows, FT Sprague, M AF Bell, J. G. Strachan, F. Roy, W. J. Matthew, C. McDonald, P. Barrows, F. T. Sprague, M. TI Evaluation of barley protein concentrate and fish protein concentrate, made from trimmings, as sustainable ingredients in Atlantic salmon (Salmo salar L.) feeds SO AQUACULTURE NUTRITION LA English DT Article DE Atlantic salmon postsmolts; barley protein concentrate; fish meal; fish meal replacement; fish protein concentrate; growth ID TROUT ONCORHYNCHUS-MYKISS; RAINBOW-TROUT; NUTRIENT DIGESTIBILITIES; SOYBEAN PROTEIN; FED DIETS; TOTAL REPLACEMENT; GROWTH; MEAL; QUALITY; SUPPLEMENTATION AB Barley protein concentrate (BPC) was tested as a protein source in the diets of Atlantic salmon post-smolts. Fish were fed one of four experimental diets consisting of a fish meal/soya protein concentrate control (CT) along with two feeds supplemented with increasing levels of BPC replacing the more costly SPC. A fourth diet partially replaced FM in the high BPC diet with a liquid fish protein concentrate (FPC) made from fish trimmings. No significant differences were observed in terms of growth at the end of the 12-week feeding period, although the protein efficiency ratio (ggaing(-1) protein consumed) was significantly lower for the control compared to fish fed diets containing either BPC only or FPC and BPC. This suggests that diets containing BPC had a beneficial effect when compared to the control diet. Furthermore, the lack of any detriment to fish growth in diets containing BPC suggests there are no significant issues regarding any negative effects of potential antinutritional factors which can otherwise be the case with other plant origin products. The data presented in this study indicate that BPC and FPC are products which could be of benefit to salmon culture, and related species, in providing a valuable new raw material to the industry. C1 [Bell, J. G.; Strachan, F.; Roy, W. J.; Matthew, C.; Sprague, M.] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland. [McDonald, P.] Scanbio Marine, Scanbio AS, Bjugn, Norway. [Barrows, F. T.] USDA ARS, Fish Technol Ctr, Bozeman, MT USA. RP Bell, JG (reprint author), Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland. EM g.j.bell@stir.ac.uk FU Scanbio A/S group (Norway) FX This study was funded by Scanbio A/S group (Norway) who also provided the FPC. We thank Rob Murray from the Institute of Aquaculture for providing the fish for the trial as well as the staff at the Machrihanish Marine Environmental Laboratory for their assistance with fish husbandry. Finally, we would like to thank Jason Frost, Carley Stone and Nic Leonard of the US Agricultural Research Service for diet manufacturing and Montanta Micobial Products who donated the BPC. NR 34 TC 0 Z9 0 U1 4 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD APR PY 2016 VL 22 IS 2 BP 326 EP 334 DI 10.1111/anu.12250 PG 9 WC Fisheries SC Fisheries GA DG8CL UT WOS:000372309900006 ER PT J AU Anuta, JD Buentello, A Patnaik, S Hume, ME Mustafa, A Gatlin, DM Lawrence, AL AF Anuta, J. D. Buentello, A. Patnaik, S. Hume, M. E. Mustafa, A. Gatlin, D. M., III Lawrence, A. L. TI Effects of dietary supplementation of a commercial prebiotic Previda((R)) on survival, growth, immune responses and gut microbiota of Pacific white shrimp, Litopenaeus vannamei SO AQUACULTURE NUTRITION LA English DT Article DE gut microbiota; innate immunity; prebiotics; shrimp ID HYBRID STRIPED BASS; X MORONE-SAXATILIS; STREPTOCOCCUS-INIAE INFECTION; MANNAN OLIGOSACCHARIDE; ONCORHYNCHUS-MYKISS; PERFORMANCE; RESISTANCE; BACTERIA; YEAST; INULIN AB A 35-day feeding trial was conducted to evaluate growth, bacterial populations of the gastrointestinal tract (GIT) and immune responses of Litopenaeus vannamei fed diets containing the commercial prebiotic Previda((R)). Diets were formulated to contain Previda((R)) at 0, 0.2, 0.5, 1.0 or 1.6gkg(-1) by weight. At the end of the study, differences in weight gain and survival among treatments were not significant (P>0.05), but denaturing gradient gel electrophoresis analysis revealed that the microbial communities in the GIT changed significantly with the inclusion of dietary Previda((R)) at different levels. Previda((R)) was therefore able to selectively modify the microbial communities in the shrimp's GIT. Although individual bacterial species were not identified, enteric populations in shrimp fed the prebiotic at similar levels of inclusion were genetically similar. In addition, shrimp fed Previda((R)) at 1.6gkg(-1) responded significantly (P<0.05) better immunologically with respect to hemocyte phagocytic capacity, haemolymph protein, hyaline cell counts and haemolymph glucose compared with shrimp fed the basal diet. Although shrimp were not exposed to virulent pathogens in this study, the observed upregulation of some of imm-une responses upon prebiotic supplementation indicates that an improved outcome of such challenges may be anticipated in Previda((R))-fed shrimp under commercial conditions. C1 [Anuta, J. D.; Patnaik, S.; Lawrence, A. L.] Texas A&M Univ Syst, Agrilife Res Mariculture Lab, Port Aransas, TX 78343 USA. [Buentello, A.; Gatlin, D. M., III] Texas A&M Univ Syst, Fac Nutr, Dept Wildlife & Fisheries Sci & Intercollegiate, College Stn, TX USA. [Buentello, A.] Schillinger Genet & Navita Premium Feed Ingredien, W Des Moines, IA USA. [Hume, M. E.] ARS, USDA, Southern Plains ARC, Food & Feed Safety Res Unit, College Stn, TX USA. [Mustafa, A.] Indiana Univ Purdue Univ, Dept Biol, Ft Wayne, IN USA. RP Lawrence, AL (reprint author), Texas A&M Univ Syst, Agrilife Res Mariculture Lab, Port Aransas, TX 78343 USA. EM smpall@yahoo.com FU Gulf Coast Shrimp Project of Texas Agrilife Research Mariculture Laboratory, Texas A&M University System [R- 9005]; United States Department of Agriculture; U.S. Marine Shrimp Farming Program; USDA/CSREES [2002-38808-01345]; Temple - Inland, Inc., Diboll, Texas FX The research was supported in part by Project R- 9005, Gulf Coast Shrimp Project of Texas Agrilife Research Mariculture Laboratory, Texas A&M University System, United States Department of Agriculture, U.S. Marine Shrimp Farming Program, USDA/CSREES Grant No. 2002-38808-01345 and Temple - Inland, Inc., Diboll, Texas. The assistance of staff and students at the Shrimp Mariculture Project of the Texas A&M University System is also greatly appreciated. NR 48 TC 0 Z9 0 U1 8 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD APR PY 2016 VL 22 IS 2 BP 410 EP 418 DI 10.1111/anu.12257 PG 9 WC Fisheries SC Fisheries GA DG8CL UT WOS:000372309900015 ER PT J AU Richardson, CM Siccardi, AJ Palle, SR Campbell, LM Puckhaber, L Stipanovic, RD Wedegaertner, TC Rathore, KS Samocha, TM AF Richardson, C. M. Siccardi, A. J. Palle, S. R. Campbell, L. M. Puckhaber, L. Stipanovic, R. D. Wedegaertner, T. C. Rathore, K. S. Samocha, T. M. TI Evaluation of ultra-low gossypol cottonseed and regular glandless cottonseed meals as dietary protein and lipid sources for Litopenaeus vannamei reared under zero-exchange conditions SO AQUACULTURE NUTRITION LA English DT Article DE cottonseed; fishmeal replacement; gossypol; Litopenaeus vannamei; RNAi; shrimp feed ID PENAEUS-VANNAMEI; JUVENILES; SQUID AB The current study evaluated transgenic cotton lines with normal levels of gossypol/terpenoids in the vegetative and floral tissues, but with ultra-low gossypol in the seeds as a replacement for glandless cottonseed meal (GCSM) and fishmeal. A 64-day growth trial evaluated the ability of cottonseed meals from a natural glandless cotton variety/mutant, two transgenic Ultra-low Gossypol Cottonseed (ULGCS) lines, a non-transgenic parental control and a commercial variety, to replace 355gkg(-1) fishmeal in a diet containing 350gkg(-1) crude protein. Juvenile Litopenaeus vannamei (1.48 +/- 0.29g) were stocked (40 shrimpm(-3)) with six replicates. No significant differences were found between all formulated diets in terms of final weight, survival and feed conversion ratio. The commercial cottonseed variety displayed a significantly lower feed efficiency ratio and protein efficiency ratio than one of the ULGCS diets. These results suggest that GCSM and/or transgenic ULGCS meals can be used to replace fishmeal in commercial shrimp diets. C1 [Richardson, C. M.; Siccardi, A. J.; Samocha, T. M.] Texas A&M AgriLife Res Mariculture Lab Flour Bluf, 4301 Waldron Rd, Corpus Christi, TX 78418 USA. [Palle, S. R.; Campbell, L. M.; Rathore, K. S.] Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX USA. [Puckhaber, L.; Stipanovic, R. D.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX USA. [Wedegaertner, T. C.] Cotton Inc, Cary, NC USA. RP Richardson, CM (reprint author), Texas A&M AgriLife Res Mariculture Lab Flour Bluf, 4301 Waldron Rd, Corpus Christi, TX 78418 USA. EM Cristina422@hotmail.com FU Cotton Incorporated; Texas A&M AgriLife Research FX Special thanks to Rebecca Lund for her assistance managing the experimental systems. This publication was supported by Cotton Incorporated and Texas A&M AgriLife Research. NR 20 TC 0 Z9 0 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD APR PY 2016 VL 22 IS 2 BP 427 EP 434 DI 10.1111/anu.12261 PG 8 WC Fisheries SC Fisheries GA DG8CL UT WOS:000372309900017 ER PT J AU D'Amore, DV Edwards, RT Biles, FE AF D'Amore, David V. Edwards, Rick T. Biles, Frances E. TI Biophysical controls on dissolved organic carbon concentrations of Alaskan coastal temperate rainforest streams SO AQUATIC SCIENCES LA English DT Article DE Dissolved organic carbon; Wetlands; Salmon; Stream biogeochemistry; Slope ID PACIFIC SALMON; MATTER CONCENTRATION; EXPORT; WATER; ECOSYSTEMS; WETLAND; UPLAND; SOILS; FLUX; CATCHMENTS AB Coastal carbon cycling models remain incomplete in key continental margins worldwide. Large quantities of labile terrestrial DOC are transferred to the Gulf of Alaska in a flow of freshwater discharge from thousands of watersheds that equals the discharge of the Mississippi River. The coastal margin of southeast Alaska and British Columbia is a potential hotspot of worldwide DOC metabolism and the mass and reactivity of DOC in rivers and estuaries of the region make quantifying and modeling DOC export a priority. Scaling DOC export requires a well-constrained model of streamwater DOC concentrations. We established models for prediction of DOC streamwater concentrations through a broad sampling of streams across a large, diverse landscape in 61 independent watersheds. Stream DOC concentrations were significantly related to the amount of wetlands in a watershed confirming the necessity of understanding the fate of the mass of carbon stored within them. Several measures of slope were useful in predictive models of streamwater DOC concentrations suggesting that slope predicts the presence of wetland soils better than current wetland map layers. We also provide evidence that suggests watersheds with large salmon spawning runs have distinct characteristics that influence the relationship between wetlands and carbon cycling that may provide additional insights into how carbon is processed. C1 [D'Amore, David V.; Edwards, Rick T.; Biles, Frances E.] US Forest Serv, Dept Agr, Pacific Northwest Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. RP D'Amore, DV (reprint author), US Forest Serv, Dept Agr, Pacific Northwest Res Stn, 11175 Auke Lake Way, Juneau, AK 99801 USA. EM ddamore@fs.fed.us; rtedwards@fs.fed.us; fbiles@fs.fed.us NR 74 TC 1 Z9 1 U1 4 U2 20 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 1015-1621 EI 1420-9055 J9 AQUAT SCI JI Aquat. Sci. PD APR PY 2016 VL 78 IS 2 BP 381 EP 393 DI 10.1007/s00027-015-0441-4 PG 13 WC Environmental Sciences; Limnology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA DH2FG UT WOS:000372599000015 ER PT J AU Juarez, RL Schwartz, MK Pilgrim, KL Thompson, DJ Tucker, SA Smith, JB Jenks, JA AF Juarez, Rebeca L. Schwartz, Michael K. Pilgrim, Kristine L. Thompson, Daniel J. Tucker, Stephanie A. Smith, Joshua B. Jenks, Jonathan A. TI Assessing temporal genetic variation in a cougar population: influence of harvest and neighboring populations SO CONSERVATION GENETICS LA English DT Article DE Cougar; Genetic variation; Black Hills; Harvest; South Dakota; Microsatellite ID LIONS PUMA-CONCOLOR; LINKAGE DISEQUILIBRIUM; MOUNTAIN LIONS; MICROSATELLITE ANALYSIS; RE-IMPLEMENTATION; N-E; SIZE; DIVERSITY; CONSERVATION; SOFTWARE AB The geography of the Black Hills region of South Dakota and Wyoming may limit connectivity for many species. For species with large energetic demands and large home ranges or species at low densities this can create viability concerns. Carnivores in this region, such as cougars (Puma concolor), have the additive effect of natural and human-induced mortality; this may act to decrease long-term viability. In this study we set out to explore genetic diversity among cougar populations in the Black Hills and surrounding areas. Specifically, our objectives were to first compare genetic variation and effective number of breeders of cougars in the Black Hills during three harvest regimes: pre (2003-2006), moderate (2007-2010), and heavy (2011-2013), to determine if harvest impacted genetic variation. Second, we compared genetic structure of the Black Hills cougar population with cougar populations in neighboring eastern Wyoming and North Dakota. Using 20 microsatellite loci, we conducted genetic analysis on DNA samples from cougars in the Black Hills (n = 675), North Dakota (n = 113), and eastern Wyoming (n = 62) collected from 2001-2013. Here we report that the Black Hills cougar population maintained genetic variation over the three time periods. Our substructure analysis suggests that the maintenance of genetic variation was due to immigration from eastern Wyoming and possibly North Dakota. C1 [Juarez, Rebeca L.; Smith, Joshua B.; Jenks, Jonathan A.] S Dakota State Univ, Dept Nat Resource Management, Brookings, SD 57007 USA. [Schwartz, Michael K.; Pilgrim, Kristine L.] US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith, Missoula, MT 59801 USA. [Thompson, Daniel J.] Wyoming Game & Fish Dept, Lander, WY 82520 USA. [Tucker, Stephanie A.] North Dakota Game & Fish Dept, Bismarck, ND 58501 USA. RP Juarez, RL (reprint author), S Dakota State Univ, Dept Nat Resource Management, Brookings, SD 57007 USA. EM juarez.rebeca1@gmail.com RI Jenks, Jonathan/B-7321-2009 FU [W-75-R]; [7545] FX Financial support for this project was funded by Federal Aid to Wildlife Restoration under project W-75-R (Study No. 7545) administered through the South Dakota Department of Game, Fish and Parks. We thank J. Alexander, S. Griffin, J. Broecher, L. Meduna, A. Rhoe, B. Tycz, M. Gottlob, B. Parr, L. Wiechmann, J. Kanta, A. Lindbloom, T. Berdan, J. Felio, K. Cudmore, and T. Solano for assistance with cougar captures. We thank all residence and property owners throughout the Black Hills for allowing access to their property. We thank K. Morgan, C. Engkjer, K. McKelvey, and J. Tucker for assistance with genetics analysis. We thank the Wyoming Game and Fish and North Dakota Game and Fish departments for providing additional DNA samples. We also thank the Civil Air Patrol pilots G. Hewett, L. Becht, R. Deweese, C. Goodrich, and M. Beason for assistance with flight time and aerial telemetry. NR 67 TC 1 Z9 1 U1 9 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD APR PY 2016 VL 17 IS 2 BP 379 EP 388 DI 10.1007/s10592-015-0790-5 PG 10 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA DG7QI UT WOS:000372278400012 ER PT J AU Lents, CA Brown-Brandl, TM Rohrer, GA Oliver, WT Freking, BA AF Lents, C. A. Brown-Brandl, T. M. Rohrer, G. A. Oliver, W. T. Freking, B. A. TI Plasma concentrations of acyl-ghrelin are associated with average daily gain and feeding behavior in grow-finish pigs SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Ghrelin; Swine; Growth; Feeding behavior; Breed; Programming ID INCREASES FOOD-INTAKE; ENDOCRINE RESPONSES; ENERGY HOMEOSTASIS; WEANLING PIGS; WEIGHT-GAIN; SECRETION; HORMONE; APPETITE; FEEDERS; ORIGINS AB The objectives of this study were to determine the effect of sex, sire line, and litter size on concentrations of acyl-ghrelin and total ghrelin in plasma of grow-finish pigs and to understand the relationship of plasma concentrations of ghrelin with feeding behavior, average daily gain (ADG), and back fat in grow-finish swine. Yorkshire-Landrace crossbred dams were inseminated with semen from Yorkshire, Landrace, or Duroc sires. Within 24 h of birth, pigs were cross-fostered into litter sizes of normal (N; >12 pigs/litter) or small (S; <= 9 pigs/litter). At 8 wk of age, pigs (n = 240) were blocked by sire breed, sex, and litter size and assigned to pens (n = 6) containing commercial feeders modified with a system to monitor feeding behavior. Total time eating, number of daily meals, and duration of meals were recorded for each individual pig. Body weight was recorded every 4 wk. Back fat and loin eye area were recorded at the conclusion of the 12-wk feeding study. A blood sample was collected at week 7 of the study to quantify concentrations of acyl- and total ghrelin in plasma. Pigs from small litters weighed more (P < 0.05) and tended (P = 0.07) to be fatter than pigs from normal litters. Postnatal litter size did not affect ADG, feeding behavior, or concentrations of ghrelin in plasma during the grow-finish phase. Barrows spent more time eating (P < 0.001) than gilts, but the number of meals and concentrations of ghrelin did not differ with sex of the pig. Pigs from Duroc and Yorkshire sires had lesser (P < 0.0001) concentrations of acyl-ghrelin than pigs from Landrace sires, but plasma concentrations of total ghrelin were not affected by sire breed. Concentrations of acyl-ghrelin were positively correlated with the number of meals and negatively correlated with meal length and ADG (P < 0.05). A larger number of short-duration meals may indicate that pigs with greater concentrations of acyl-ghrelin consumed less total feed, which likely explains why they were leaner and grew more slowly. Acyl-ghrelin is involved in regulating feeding behavior in pigs, and measuring acyl-ghrelin is important when trying to understand the role of this hormone in swine physiology. Published by Elsevier Inc. C1 [Lents, C. A.; Brown-Brandl, T. M.; Rohrer, G. A.; Oliver, W. T.; Freking, B. A.] USDA, ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Lents, CA (reprint author), USDA, ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM clay.lents@ars.usda.gov NR 37 TC 0 Z9 0 U1 1 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD APR PY 2016 VL 55 BP 107 EP 113 DI 10.1016/j.domaniend.2015.12.005 PG 7 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA DH0NU UT WOS:000372481700013 PM 26808977 ER PT J AU Foote, AP Tait, RG Keisler, DH Hales, KE Freetly, HC AF Foote, A. P. Tait, R. G., Jr. Keisler, D. H. Hales, K. E. Freetly, H. C. TI Leptin concentrations in finishing beef steers and heifers and their association with dry matter intake, average daily gain, feed efficiency, and body composition SO DOMESTIC ANIMAL ENDOCRINOLOGY LA English DT Article DE Beef cattle; Feed efficiency; Feed intake; Growth; Leptin ID CARCASS COMPOSITION; CATTLE; RUMINANTS; GROWTH AB The objective of this experiment was to determine the association of circulating plasma leptin concentrations with production and body composition measures of finishing beef steers and heifers and to determine if multiple sampling time points improve the associations of plasma leptin concentrations with production and body composition traits. Individual dry matter intake (DMI) and ADG were determined for 84 d using steers and heifers (n = 127 steers and n = 109 heifers). Blood was collected on day 0, day 42, and day 83 for determination of plasma leptin concentrations. Leptin concentrations were greater in heifers than those in steers on day 0 (P < 0.001 for sex by day interaction), and leptin concentrations increased in both sexes but were not different from each other on day 83. Leptin concentrations at all 3 time points and the mean were shown to be positively associated with DMI (P <= 0.006), whereas the mean leptin concentration explaining 8.3% of the variance of DMI. Concentrations of leptin at day 42, day 83, and the mean of all 3 time points were positively associated with ADG (P <= 0.011). Mean leptin concentration was negatively associated with gain:feed ratio and positively associated with residual feed intake (RFI), indicating that more efficient cattle had lower leptin concentrations. However, leptin concentrations explained very little of the variation in residual feed intake (<= 3.2% of the variance). Leptin concentrations were positively associated with body fat measured by ultrasonography at the 12th rib and over the rump (P < 0.001), with the mean leptin concentration explaining 21.9% and 12.7% of the variance in 12th rib and rump fat thickness, respectively. The same trend was observed with carcass composition where leptin concentrations were positively associated with 12th rib fat thickness, USDA-calculated yield grade (YG), and marbling score (P <= 0.006) and mean leptin concentration explained 16.8,18.2, and 4.6% of the variance for 12th rib fat thickness, yield grade, and marbling score, respectively. Given these and previous results, it appears that leptin physiology could be a candidate for mechanisms that contribute to feed intake and feed efficiency variation in beef cattle. Published by Elsevier Inc. C1 [Foote, A. P.; Tait, R. G., Jr.; Hales, K. E.; Freetly, H. C.] USDA, ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Keisler, D. H.] Univ Missouri, Dept Anim Sci, Columbia, MO 65211 USA. RP Foote, AP (reprint author), USDA, ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Andrew.Foote@ars.usda.gov RI Keisler, Duane/C-2746-2011 OI Keisler, Duane/0000-0002-8792-7030 FU NIFA grant through the National Program for Genetic Improvement of Feed Efficiency in Beef Cattle [2011-68004-30214] FX This project was partially funded by NIFA grant no. 2011-68004-30214 through the National Program for Genetic Improvement of Feed Efficiency in Beef Cattle. NR 14 TC 1 Z9 1 U1 3 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0739-7240 EI 1879-0054 J9 DOMEST ANIM ENDOCRIN JI Domest. Anim. Endocrinol. PD APR PY 2016 VL 55 BP 136 EP 141 DI 10.1016/j.domaniend.2015.12.007 PG 6 WC Agriculture, Dairy & Animal Science; Endocrinology & Metabolism SC Agriculture; Endocrinology & Metabolism GA DH0NU UT WOS:000372481700016 PM 26851619 ER PT J AU Gondim, LFP Wolf, A Vrhovec, MG Pantchev, N Bauer, C Langenmayer, MC Bohne, W Teifke, JP Dubey, JP Conraths, FJ Schares, G AF Gondim, Luis F. P. Wolf, Alexander Vrhovec, Majda G. Pantchev, Nikola Bauer, Christian Langenmayer, Martin C. Bohne, Wolfgang Teifke, Jens P. Dubey, Jitender P. Conraths, Franz J. Schares, Gereon TI Characterization of an IgG monoclonal antibody targeted to both tissue cyst and sporocyst walls of Toxoplasma gondii SO EXPERIMENTAL PARASITOLOGY LA English DT Article DE Toxoplasma; Monoclonal antibody; Oocyst; Sporocyst; Tissue cyst; Immunoblot ID HAMMONDIA-HAMMONDI; NEOSPORA-CANINUM; OOCYST WALL; IN-VITRO; MOLECULAR CHARACTERIZATION; IMMUNOMAGNETIC SEPARATION; BESNOITIA-BESNOITI; INFECTED-TISSUES; ANTIGEN; PCR AB Toxoplasma gondii infects animals habiting terrestrial and aquatic environments. Its oocysts and tissue cysts are important for the horizontal transmission of this parasite. The oocyst and tissue cyst walls are crucial for the ability of the parasite to persist in the environment or in animal tissues, respectively. However, the composition of these walls is not well understood. We report the generation of monoclonal antibodies directed against wall components using mice immunized with oocyst antigens of T gondii. One monoclonal antibody (mAb) G1/19 reacted solely with T. gondii sporozoites. The respective antigen had a relative molecular weight (Mr) of 30 kDa. MAb G1/19 failed to react with sporozoites of any other coccidian parasite species tested (Hammondia hammondi, Hammondia heydorni, Cystoisospora felis, Eimeria bovis, Sarcocystis sp.). Another mAb, designated K8/15-15, recognized antigens in sporocyst walls of the parasite and in the walls of in vivo or in vitro produced tissue cysts, as demonstrated by immunofluorescence and immunoblot assays. Antigens of 80 to a high molecular weight protein of about 350 kDa Mr were recognized by this antibody using antigen extracts from sporocysts, and from in vitro or in vivo generated tissue cysts of the parasite. Tissue cyst and sporocyst walls of H. hammondi and H. heydorni, and tissue cysts of Neospora caninum were also recognized by mAb K8/15-15. Sporocyst walls of C. felis also reacted to this mAb. The cyst walls of Sarcocystis sp. and Besnoitia besnoiti were not recognized by mAb K8/15-15. Reactivity by a single mAb against T. gondii antigens in tissue cysts and sporocysts had not been reported previously. MAb K8/15-15 may be a practical tool for the identification of both cysts and sporocysts of the parasite, and may also be potentially employed in proteomic studies on the identification of new components of the cyst and sporocyst walls of T. gondii. (C) 2016 Elsevier Inc. All rights reserved. C1 [Gondim, Luis F. P.; Wolf, Alexander; Conraths, Franz J.; Schares, Gereon] Friedrich Loeffler Inst, Inst Epidemiol, Fed Res Inst Anim Hlth, Sudufer 10, D-17493 Greifswald, Germany. [Teifke, Jens P.] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Dept Expt Anim Facil & Biorisk Management, D-17493 Greifswald, Germany. [Vrhovec, Majda G.; Pantchev, Nikola] IDEXX Labs, D-71636 Ludwigsburg, Germany. [Gondim, Luis F. P.] Univ Fed Bahia, Escola Med Vet & Zootecnia, Dept Anat Patol & Clin, Av Adhemar Barros 500, BR-40170110 Salvador, BA, Brazil. [Bauer, Christian] Univ Giessen, Inst Parasitol, D-35390 Giessen, Germany. [Langenmayer, Martin C.] Univ Munich, Inst Vet Pathol, Munich, Germany. [Langenmayer, Martin C.] Univ Munich, Inst Infect Dis & Zoonoses, Munich, Germany. [Bohne, Wolfgang] Univ Gottingen, Dept Microbiol, Kreuzbergring 57, D-37075 Gottingen, Germany. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Gondim, LFP (reprint author), Univ Fed Bahia, Escola Med Vet & Zootecnia, Dept Anat Patol & Clin, Av Adhemar Barros 500, BR-40170110 Salvador, BA, Brazil. EM pita@ufba.br OI Gondim, Luis/0000-0003-3741-121X; Schares, Gereon/0000-0002-3217-289X; Conraths, Franz/0000-0002-7400-9409 FU Alexander von Humboldt Foundation, Germany; German Federal Ministry of Education and Research [01KI0765, 01KI1002F]; Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), Brazil [PET0003/2012]; George Forster Research Fellowship from Alexander von Humboldt Foundation from Brazil; CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) from Brazil FX This study received financial support from the following sources: Alexander von Humboldt Foundation, Germany, German Federal Ministry of Education and Research (Toxonet01 and Toxonet02) by funds to Dr. Schares (01KI0765; 01KI1002F), and Fundacao de Amparo a Pesquisa do Estado da Bahia (FAPESB), Brazil by funds to Dr. Gondim (PET0003/2012). L.F.P. Gondim was recipient of a George Forster Research Fellowship from the Alexander von Humboldt Foundation and CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior) from Brazil. We thank Andrea Barwald and Gabriele Czerwinski (Friedrich-Loeffler-Institut) for excellent technical assistance. We are thankful to M. McAllister (University of Adelaide, Australia) for providing anti-BAG1 serum. NR 38 TC 0 Z9 0 U1 3 U2 9 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 EI 1090-2449 J9 EXP PARASITOL JI Exp. Parasitol. PD APR PY 2016 VL 163 BP 46 EP 56 DI 10.1016/j.exppara.2016.01.014 PG 11 WC Parasitology SC Parasitology GA DH3DM UT WOS:000372667700007 PM 26836446 ER PT J AU Latta, GS Adams, DM Bell, KP Kline, JD AF Latta, Gregory S. Adams, Darius M. Bell, Kathleen P. Kline, Jeffrey D. TI Evaluating land-use and private forest management responses to a potential forest carbon offset sales program in western Oregon (USA) SO FOREST POLICY AND ECONOMICS LA English DT Article DE Carbon markets; Carbon storage; Climate change; Silviculture; Forest land development ID SEQUESTERING CARBON; COST; SEQUESTRATION; SINKS; ECONOMICS; SUBSIDIES; SERVICES; IMPACTS; CLIMATE; MARKETS AB We describe the use of linked land-use and forest sector models to simulate the effects of carbon offset sales on private forest owners' land-use and forest management decisions in western Oregon (USA). Our work focuses on forest management decisions rather than afforestation, allows full forest sector price adjustment to land-use changes, and incorporates time-dependent costs and restrictions of offset programs. The land-use model utilizes structure count data on some 21,000 plots spanning 30 years. The intertemporal optimizing forest sector model employs mill-level demand and FIA plot-level inventory. Our linked simulation modeling projects that an offset sales program could reduce forest land loss to development in western Oregon by about 4700 acres over the 2010-2060 simulation period for each $1 increase in the carbon price. At $10 per tonne CO2, regional private carbon stocks would be roughly stabilized at current levels over the period to 2060. Rotations would lengthen on enrolled lands, as expected, but use of planting, thinning and uneven-aged management would decline. Published by Elsevier B.V. C1 [Latta, Gregory S.; Adams, Darius M.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Bell, Kathleen P.] Univ Maine, Sch Econ, Orono, ME USA. [Kline, Jeffrey D.] US Forest Serv, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR USA. RP Kline, JD (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR USA. EM jkline@fs.fed.us FU U.S. Environmental Protection Agency [DW-12-92298001] FX The research described in this article was funded, in part, by the U.S. Environmental Protection Agency through Interagency Agreement #DW-12-92298001. The views expressed in this article are those of the authors and do not necessarily reflect the views or policies of the EPA. NR 47 TC 0 Z9 0 U1 3 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 EI 1872-7050 J9 FOREST POLICY ECON JI Forest Policy Econ. PD APR PY 2016 VL 65 BP 1 EP 8 DI 10.1016/j.forpol.2016.01.004 PG 8 WC Forestry SC Forestry GA DH3IG UT WOS:000372680100001 ER PT J AU Zawahri, NA Dinar, A Nigatu, G AF Zawahri, Neda A. Dinar, Ariel Nigatu, Getachew TI Governing international freshwater resources: an analysis of treaty design SO INTERNATIONAL ENVIRONMENTAL AGREEMENTS-POLITICS LAW AND ECONOMICS LA English DT Article DE International rivers; Treaty design; Negotiation context; Bilateral treaties; Multilateral and basin-wide treaties ID ENVIRONMENTAL AGREEMENTS; INSTITUTIONAL DESIGN; COOPERATION; RIVER; MANAGEMENT; ANARCHY; BASIN; MULTILATERALISM; ENFORCEMENT; STRATEGIES AB International rivers create complex relationships between their riparian states, which can contribute to economic, political, and social losses. Treaties provide a means for states to coordinate their actions in managing international river disputes to minimize these losses. However, there is little knowledge about treaty content and the factors influencing treaty design. We test whether a relationship exists between the challenges of negotiating, complying, and distributing the gains in bilateral, multilateral, and basin-wide negotiation contexts and the depth of cooperation along with the degree of institutionalization. While the great challenges confronting multilateral or basin-wide negotiations can produce treaties that focus on joint gains and shallow cooperation to secure the signature of riparians, we find that they can also provide opportunities for deeper, more behavior-altering, cooperation. To manage the difficulties of maintaining multilateral cooperation, we find a higher degree of institutionalization. We also find that bilateral negotiations provide states with opportunities for deeper cooperation, but a lower degree of institutionalization. C1 [Zawahri, Neda A.] Cleveland State Univ, Dept Polit Sci, 2121 Euclid Ave RT 1757, Cleveland, OH 44115 USA. [Dinar, Ariel] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Nigatu, Getachew] USDA, Market & Trade Econ Div, Econ Res Serv, Economist, 335 E St, Washington, DC 20024 USA. RP Zawahri, NA (reprint author), Cleveland State Univ, Dept Polit Sci, 2121 Euclid Ave RT 1757, Cleveland, OH 44115 USA. EM N.zawahri@csuohio.edu; Ariel.dinar@ucr.edu NR 72 TC 3 Z9 3 U1 1 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1567-9764 EI 1573-1553 J9 INT ENVIRON AGREEM-P JI Int. Environ. Agreem.-Polit. Law Econom. PD APR PY 2016 VL 16 IS 2 BP 307 EP 331 DI 10.1007/s10784-014-9259-0 PG 25 WC Economics; Environmental Studies; Law; Political Science SC Business & Economics; Environmental Sciences & Ecology; Government & Law GA DG7DQ UT WOS:000372245400007 ER PT J AU Geng, P Harnly, JM Chen, P AF Geng, Ping Harnly, James M. Chen, Pei TI Differentiation of bread made with whole grain and refined wheat (T. aestivum) flour using LC/MS-based chromatographic fingerprinting and chemometric approaches SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Alk(en)ylresorcinols; Phosphatidylethanolamine; Diglycerides; UHPLC-HRMS; APCI; FCMS; PCA; SIMCA; Food composition; Food analysis ID IONIZATION MASS-SPECTROMETRY; PLANT OILS; LIPIDS; ALKYLRESORCINOLS; PHOSPHOLIPIDS; HPLC; ALK(EN)YLRESORCINOLS; TRIACYLGLYCEROL; CONSUMPTION; COMPONENTS AB A fuzzy chromatography mass spectrometric (FCMS) fingerprinting method combined with chemometric analysis was established to differentiate between breads made from whole wheat (WW) flour and refined wheat (RW) flour. The chemical compositions of the bread samples were profiled using ultrahigh-performance liquid chromatography with high-resolution accurate-mass multistage mass spectrometry (UHPLC-HRAM-MSn) with atmospheric pressure chemical ionisation (APCI) in positive ionisation mode. Principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA) of the FCMS fingerprints revealed the components responsible for the chemical differences between WW and RW flour/bread samples. Alk(en)ylresorcinols (ARs) were demonstrated to be the most important markers for differentiation between WW and RW flour/breads. Diglycerides (DGs), and phosphatidylethanolamine (PE) also contributed significantly to the classification. Bread crumb and crust showed no difference in chemical profiles in this study. SIMCA, using WW modelling, could be a potent and robust tool for authentication of WW breads. Published by Elsevier Inc. C1 [Geng, Ping; Harnly, James M.; Chen, Pei] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Bldg 161,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Chen, P (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Bldg 161,BARC East,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; National Institutes of Health FX The authors would like to thank Ms. Shirley Cabiness for her excellent technical assistance in bread baking. This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health. NR 36 TC 2 Z9 2 U1 6 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD APR PY 2016 VL 47 BP 92 EP 100 DI 10.1016/j.jfca.2015.12.010 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DG9CL UT WOS:000372380300013 ER PT J AU Liu, SQ Skory, C Qureshi, N Hughes, S AF Liu, Siqing Skory, Chris Qureshi, Nasib Hughes, Stephen TI The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance SO JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Lactobacillus buchneri; Ethanol tolerance; yajC; Membrane protein; Escherichia coli ID NRRL B-30929; MUTATIONS; SEQUENCE; OPERON; FORMS; CD034 AB The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % ethanol. The yajC gene encodes the smaller subunit of the preprotein translocase complex, which interacts with membrane protein SecD and SecF to coordinate protein transport and secretion across cytoplasmic membrane in Escherichia coli. The YajC protein was linked to sensitivity to growth temperatures in E. coli, involved in translocation of virulence factors during Listeria infection, and stimulating a T cell-mediated response of Brucella abortus. In this study, the L. buchneri yajC gene was over-expressed in E. coli. The strain carrying pET28byajC that produces YajC after isopropyl beta-d-1-thiogalactopyranoside induction showed tolerance to 4 % ethanol in growth media, compared to the control carrying pET28b. This is the first report linking YajC to ethanol stress and tolerance. C1 [Liu, Siqing; Skory, Chris; Hughes, Stephen] ARS, USDA, Renewable Prod Technol Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Qureshi, Nasib] ARS, USDA, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. RP Liu, SQ (reprint author), ARS, USDA, Renewable Prod Technol Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Siqing.liu@ars.usda.gov NR 23 TC 0 Z9 0 U1 2 U2 8 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1367-5435 EI 1476-5535 J9 J IND MICROBIOL BIOT JI J. Ind. Microbiol. Biotechnol. PD APR PY 2016 VL 43 IS 4 BP 441 EP 450 DI 10.1007/s10295-015-1730-6 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DH1IZ UT WOS:000372538900002 PM 26790414 ER PT J AU Montalva, C Luz, C Humber, RA AF Montalva, C. Luz, C. Humber, R. A. TI Neozygites osornensis (Neozygitales: Neozygitaceae) Infecting Cinara sp (Hemiptera: Aphididae) in Brazil SO NEOTROPICAL ENTOMOLOGY LA English DT Article DE Aphid pathogen; Entomophthorales; natural enemy; Entomophthoromycota; Cypress aphid ID ENTOMOPHTHORALES; FUNGI AB The genus Neozygites has been known in Brazil until now only on mites, and this is its first report on aphids in Brazil. Tree-dwelling aphids (Cinara sp.) on a cypress tree were regularly monitored for entomopathogenic fungi in the city of Terezpolis de Goias in Central Brazil between July 2014 and April 2015. During the survey, mycosed aphids were found attached to twigs. The fungus was identified morphologically as Neozygites osornensis. No in vitro cultures were established from infected aphids. The finding reported here suggests that special attention should be paid in the future for the possible occurrences of Neozygites species wherever cinaran aphids occur around the globe. C1 [Montalva, C.; Luz, C.] Univ Fed Goias, Inst Patol Trop & Saude Publ, Lab Patol Invertebrados, Goiania, Go, Brazil. [Humber, R. A.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Montalva, C (reprint author), Univ Fed Goias, Inst Patol Trop & Saude Publ, Lab Patol Invertebrados, Goiania, Go, Brazil. EM montalva.cristian@gmail.com FU CAPES Coordination of the Improvement of Higher Education; CSF; PVE [71/2013]; [149988] FX This study was supported by CAPES Coordination of the Improvement of Higher Education, CSF, PVE 71/2013, 149988. NR 23 TC 0 Z9 0 U1 3 U2 6 PU ENTOMOLOGICAL SOC BRASIL PI LONDRINA, PA CAIXA POSTAL 481, 86001-970 LONDRINA,, PR, BRAZIL SN 1519-566X EI 1678-8052 J9 NEOTROP ENTOMOL JI Neotrop. Entomol. PD APR PY 2016 VL 45 IS 2 BP 227 EP 230 DI 10.1007/s13744-016-0369-y PG 4 WC Entomology SC Entomology GA DG7WH UT WOS:000372293900015 PM 26868651 ER PT J AU Engeman, RM Addison, D Griffin, JC AF Engeman, Richard M. Addison, David Griffin, J. C. TI Defending against disparate marine turtle nest predators: nesting success benefits from eradicating invasive feral swine and caging nests from raccoons SO ORYX LA English DT Article DE Bioeconomics; eradication; Florida; green turtle; invasive species; Keewaydin Island; loggerhead turtle; threatened species ID FISHERIES BYCATCH; SEA-TURTLES; MANAGEMENT; CONSERVATION; DEPREDATION; FLORIDA AB Nest predation can threaten marine turtle nesting success, and having to address dissimilar predator species complicates nest protection efforts. On Florida's Keewaydin Island predation by raccoons Procyon lotor and invasive feral swine Sus scrofa are disparate, significant threats to marine turtle nests. Using. years of nesting data (mostly for loggerhead marine turtles Caretta caretta) we examined the impacts of swine predation on nests and the benefits of swine eradication, caging nests to protect them from raccoon predation, and the effects of nest caging on swine predation. Nest predation by swine began in mid nesting season 2007, after which swine quickly annihilated all remaining marine turtle nests. During 2005-2010 raccoon predation rates for caged nests (0.7-20.4%) were significantly lower than for uncaged nests (5.6-68.8%) in every year except 2009, when little raccoon predation occurred. The proportions of eggs lost from raccoon-predated nests did not differ between caged and uncaged nests. Caging did not prevent destruction by swine but median survival time for caged nests was 11.5 days longer than for uncaged nests, indicating that caged eggs in nests have a greater chance of hatching before being predated by swine. The financial cost of the eradication of swine greatly outweighed the value of hatchlings lost to swine predation in 2007. C1 [Engeman, Richard M.] Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Addison, David] Conservancy Southwest Florida, Naples, FL USA. [Griffin, J. C.] USDA APHIS Wildlife Serv, Gainesville, FL USA. [Griffin, J. C.] Moody Air Force Base, Valdosta, GA USA. RP Engeman, RM (reprint author), Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM richard.m.engeman@aphis.usda.gov FU Rookery Bay National Estuarine Research Reserve; Marine Turtle Grants Program FX Funding for the feral swine abatement on Keewaydin Island was provided by the Rookery Bay National Estuarine Research Reserve and through a grant to the Conservancy of Southwest Florida by the Marine Turtle Grants Program. We thank Gary Lytton, Jeffrey Carter and Greg Curry for their support for the Keewaydin turtles and for their initiative, the Conservancy of Southwest Florida's 2007 marine turtle field staff (Sarah Funck, Kate McCellan, Sedonia Steininger and Michelle Wcisel) for their perseverance in collecting the data that made our analysis possible, and David Shindle of the Conservancy's Science Department for helping us document the magnitude of the feral swine population in the early stages of this project. NR 44 TC 0 Z9 0 U1 12 U2 27 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0030-6053 EI 1365-3008 J9 ORYX JI Oryx PD APR PY 2016 VL 50 IS 2 BP 289 EP 295 DI 10.1017/S0030605314000805 PG 7 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DH1DZ UT WOS:000372525900022 ER PT J AU Chen, TA Baranowski, T Moreno, JP O'Connor, TM Hughes, SO Baranowski, J Woehler, D Kimbro, RT Johnston, CA AF Chen, T. -A. Baranowski, T. Moreno, J. P. O'Connor, T. M. Hughes, S. O. Baranowski, J. Woehler, D. Kimbro, R. T. Johnston, C. A. TI Obesity status transitions across the elementary years: use of Markov chain modelling SO PEDIATRIC OBESITY LA English DT Article DE Childhood obesity; longitudinal; Markov chain; weight gain ID CATEGORICAL TIME-SERIES; CHILDHOOD OBESITY; SCHOOL-CHILDREN; SOCIOECONOMIC-STATUS; SEASONAL-VARIATION; SLEEP DURATION; PHYSICAL-ACTIVITY; SUMMER VACATION; MIDDLE SCHOOL; WEIGHT-GAIN AB ObjectiveThe objective of this study was to assess overweight and obesity status transition probabilities using first-order Markov transition models applied to elementary school children. MethodComplete longitudinal data across 11 assessments were available from 1494 elementary school children (from 7599 students in 41 out of 45 schools in a Southeast Texas school district) from kindergarten to the beginning of the fifth grade. Heights and weights were measured by trained school nurses using standard procedures at the beginning and end of each school year for the 11 consecutive assessments. To estimate the transition probabilities, first-order three-state (healthy weight, overweight and obese) Markov transition models were fit to the longitudinal weight status data of all assessment periods. ResultsWhile there was a gradual shift to more children in the overweight or obese category over 5 years, children were most likely to stay in the same weight category as the previous assessment. A consistent seasonal difference in the probability of changing weight status category was seen, with a greater probability of becoming overweight and obese during the summer compared with the school year. The transition probabilities to obesity were higher among boys, Hispanic and non-Hispanic Black, and lower socioeconomic status children. ConclusionsThis study provides the first application of a Markov transition model to child weight status data. The transitions into the overweight and obese categories were small, but persistent, with smaller percentages transitioning out of overweight or obese. Early monitoring and summer intervention strategies are needed to prevent the slow, but relentless, transition into the overweight and obese categories. C1 [Chen, T. -A.; Baranowski, T.; Moreno, J. P.; O'Connor, T. M.; Hughes, S. O.; Baranowski, J.; Woehler, D.; Kimbro, R. T.; Johnston, C. A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St,Rm 4012, Houston, TX 77030 USA. Oliver Fdn, Houston, TX USA. Rice Univ, Dept Sociol, Houston, TX USA. Baylor Coll Med, Dept Med, Houston, TX USA. RP Chen, TA (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St,Rm 4012, Houston, TX 77030 USA. EM anntzuac@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU Oliver Foundation FX This study was supported by a grant from the Oliver Foundation, and institutional support from the U.S. Department of Agriculture, Agricultural Research Service (Cooperative Agreement 58-6250-0-008). The authors thank Sandy Bristow, Sonya Kaster, RD, LDN, SNS, and Thomas R. Woehler, MD, from the Oliver Foundation for their dedication to improving the health of children. The Oliver Foundation assisted with study concept and design and assisted in supervision and implementation of the study procedures. They did not participate in analysis or interpretation of the data. DW from the Oliver Foundation provided a critical review of the paper. NR 35 TC 2 Z9 2 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-6310 EI 2047-6302 J9 PEDIATR OBES JI Pediatr. Obes. PD APR PY 2016 VL 11 IS 2 BP 88 EP 94 DI 10.1111/ijpo.12025 PG 7 WC Pediatrics SC Pediatrics GA DG8UC UT WOS:000372358100002 PM 25854860 ER PT J AU Calderon, FJ Nielsen, D Acosta-Martinez, V Vigil, MF Lyons, D AF Calderon, Francisco J. Nielsen, David Acosta-Martinez, Veronica Vigil, Merle F. Lyons, Drew TI Cover Crop and Irrigation Effects on Soil Microbial Communities and Enzymes in Semiarid Agroecosystems of the Central Great Plains of North America SO PEDOSPHERE LA English DT Article DE crop rotation; enzyme activity; FAME profile; fatty acid methyl ester; winter wheat ID LAND-USE; BETA-GLUCOSIDASE; ORGANIC-CARBON; WINTER-WHEAT; TILLAGE; FALLOW; AVAILABILITY; MANAGEMENT; ECOSYSTEM; PRECIPITATION AB Cover crops can have beneficial effects on soil microbiology by increasing carbon (C) supply, but these beneficial effects can be modulated by precipitation conditions. The objective of this study was to compare a fallow-winter wheat (Triticum aestivum L.) rotation to several cover crop-winter wheat rotations under rainfed and irrigated conditions in the semiarid US High Plains. Experiments were carried out at two sites, Sidney in Nebraska, and Akron in Colorado, USA, with three times of soil sampling in 2012-2013 at cover crop termination, wheat planting, and wheat maturity. The experiments included four single-species cover crops, a 10-species mixture, and a fallow treatment. The variables measured were soil C and nitrogen (N), soil community structure by fatty acid methyl ester (FAME) profiles, and soil beta-glucosidase, beta-glucosaminidase, and phosphodiesterase activities. The fallow treatment, devoid of living plants, reduced the concentrations of most FAMEs at cover crop termination. The total FAME concentration was correlated with cover crop biomass (R = 0.62 at Sidney and 0.44 at Akron). By the time of wheat planting, there was a beneficial effect of irrigation, which caused an increase in mycorrhizal and protozoan markers. At wheat maturity, the cover crop and irrigation effects on soil FAMEs had subsided, but irrigation had a positive effect on the P-glucosidase and phosphodiesterase activities at Akron, which was the drier of the two sites. Cover crops and irrigation were slow to impact soil C concentration. Our results show that cover crops had a short-lived effect on soil microbial communities in semiarid wheat-based rotations and irrigation could enhance soil enzyme activity. In the semiarid environment, longer time spans may have been needed to see beneficial effects of cover crops on soil microbial community structure, soil enzyme activities, and soil C sequestration. C1 [Calderon, Francisco J.; Nielsen, David; Vigil, Merle F.] ARS, USDA, Cent Great Plains Res Stn, Akron, CO 80720 USA. [Acosta-Martinez, Veronica] ARS, USDA, Cropping Syst Res Lab, Wind Eros & Water Conservat Unit, Lubbock, TX 79415 USA. [Lyons, Drew] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Calderon, FJ (reprint author), ARS, USDA, Cent Great Plains Res Stn, Akron, CO 80720 USA. EM francisco.calderon@ars.usda.gov NR 47 TC 1 Z9 1 U1 21 U2 42 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD APR PY 2016 VL 26 IS 2 BP 192 EP 205 DI 10.1016/S1002-0160(15)60034-0 PG 14 WC Soil Science SC Agriculture GA DH4NP UT WOS:000372763000005 ER PT J AU Chen, J Liu, ZS Li, XY Liu, P Jiang, JC Nie, XA AF Chen, Jie Liu, Zengshe Li, Xiaoying Liu, Peng Jiang, Jianchun Nie, Xiaoan TI Thermal behavior of epoxidized cardanol diethyl phosphate as novel renewable plasticizer for poly(vinyl chloride) SO POLYMER DEGRADATION AND STABILITY LA English DT Article DE Poly(vinyl chloride) (PVC); Natural plasticizer; Cardanol; Thermal stability ID POLYVINYL-CHLORIDE; NATURAL-RUBBER; POLYMER BLENDS; OLEIC-ACID; SEED OIL; DEGRADATION; RESOURCES; ALTERNATIVES; OPTIMIZATION; DERIVATIVES AB A novel plasticizer, epoxidized cardanol diethyl phosphate (ECEP), based on cardanol was synthesized. Chemical structure of ECEP was characterized by fourier transform infrared (FTIR), H-1-nuclear magnetic resonance(H-1 NMR) and C-13-nuclear magnetic resonance(C-13 NMR). Effects of ECEP substitution for the petroleum-based plasticizer dioctyl phthalate (DOP) in soft poly(vinyl chloride) (PVC) films were studied. Thermal stability and mechanical properties before and after aging of PVC films plasticized with different content of ECEP were investigated with thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA) and tensile tests. The results indicated that PVC films plasticized with ECEP showed increased thermal stability. When DOP was substituted for ECEP completely, glass transition temperature (T-g) of PVC films decreased from 41.07 degrees C to 33.20 degrees C, the 10% and 50% weight loss temperatures (T-10 and T-50) increased by 31.57 degrees C and 23.09 degrees C respectively. The mechanical properties of PVC films before and after aging were also investigated. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chen, Jie; Li, Xiaoying; Liu, Peng; Jiang, Jianchun; Nie, Xiaoan] Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Natl Engn Lab Biomass Chem Utilizat, Key Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China. [Liu, Zengshe] ARS, USDA, Natl Ctr Agr Utilizat Res, Biooils Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. [Chen, Jie] USDA, Craig, AK USA. RP Nie, XA (reprint author), Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Dept Oil Chem, Nanjing 210042, Jiangsu, Peoples R China.; Jiang, JC (reprint author), Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Dept Bioresource Chem, Nanjing 210042, Jiangsu, Peoples R China. EM bio-enegry@163.com; niexiaoan@126.com FU National "Twelfth Five-Year" Plan for Science & Technology Support [2015BAD15B08] FX The authors are grateful for the financial support from National "Twelfth Five-Year" Plan for Science & Technology Support (grant number: 2015BAD15B08). The authors also gratefully acknowledge Mr. Daniel Knetzer for help in DMA and TGA analysis and Mrs. Kathy Hornback for help in preparing PVC test specimens. NR 47 TC 3 Z9 3 U1 3 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0141-3910 EI 1873-2321 J9 POLYM DEGRAD STABIL JI Polym. Degrad. Stabil. PD APR PY 2016 VL 126 BP 58 EP 64 DI 10.1016/j.polymdegradstab.2016.01.018 PG 7 WC Polymer Science SC Polymer Science GA DH4NZ UT WOS:000372764000008 ER PT J AU Chirici, G Mura, M McInerney, D Py, N Tomppo, EO Waser, LT Travaglini, D McRoberts, RE AF Chirici, Gherardo Mura, Matteo McInerney, Daniel Py, Nicolas Tomppo, Erkki O. Waser, Lars T. Travaglini, Davide McRoberts, Ronald E. TI A meta-analysis and review of the literature on the k-Nearest Neighbors technique for forestry applications that use remotely sensed data SO REMOTE SENSING OF ENVIRONMENT LA English DT Review DE k-Nearest Neighbors; Forestry applications; Review; Meta-analysis ID ABOVEGROUND BIOMASS; SATELLITE IMAGERY; MODERATE RESOLUTION; ANCILLARY DATA; BASAL AREA; LANDSAT-TM; INVENTORY; VOLUME; ATTRIBUTES; VARIABLES AB The k-Nearest Neighbors (k-NN) technique is a popular method for producing spatially contiguous predictions of forest attributes by combining field and remotely sensed data. In the framework of Working Group 2 of COST Action FP1001, we reviewed the scientific literature for forestry applications of k-NN. Information available in scientific publications on this topic was used to populate a database that was then used as the basis for a meta analysis. We extracted qualitative and quantitative information from 260 experimental tests described in 148 scientific papers. The papers represented a geographic range of 26 countries and a temporal range from 1981 to 2013. Firstly, we describe the literature search and the information extracted and analyzed. Secondly, we report the results of the meta-analysis, especially with respect to estimation accuracies reported for k-NN applications for different configurations, different forest environments, and different input information. We also provide a summary of results that may reasonably be expected for those planning a k-NN application using remotely sensed data from different sensors and for different forest attributes. Finally, we identify some methodological publications that have advanced the state of the science with respect to k-NN. (C) 2016 Elsevier Inc. All rights reserved. C1 [Chirici, Gherardo; Mura, Matteo; Travaglini, Davide] Univ Florence, Dept Agr Food & Forestry Syst, Via San Bonaventura 13, I-50145 Florence, Italy. [McInerney, Daniel] Coillte Teoranta, Cork, Ireland. [Py, Nicolas] Inst Natl Informat Geog & Forestiere, Paris, France. [Tomppo, Erkki O.] Nat Resources Inst, Helsinki, Finland. [Waser, Lars T.] WSL, Swiss Fed Inst Forest Snow & Landscape Res, Birmensdolf, Switzerland. [McRoberts, Ronald E.] US Forest Serv, USDA, No Res Stn, New York, NY USA. RP Chirici, G (reprint author), Univ Florence, Dept Agr Food & Forestry Syst, Via San Bonaventura 13, I-50145 Florence, Italy. EM gherardo.chirici@unifi.it RI Waser, Lars/D-5937-2011; Travaglini, Davide/F-1845-2013 OI Waser, Lars/0000-0002-2609-9147; Travaglini, Davide/0000-0003-0706-2653 FU French National Research Agency [ANR-2010-BIOE-008 FORESEE]; Italian Ministry of Research and Education [2012EWEY2S] FX Part of the work was supported by the French National Research Agency (project ANR-2010-BIOE-008 FORESEE) and by the Italian Ministry of Research and Education (project PRIN2012 "Development of innovative methods for forest ecosystems monitoring based on remote sensing", grant 2012EWEY2S, project coordinator: G. Chirici). NR 53 TC 4 Z9 4 U1 4 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD APR PY 2016 VL 176 BP 282 EP 294 DI 10.1016/j.rse.2016.02.001 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DG9DO UT WOS:000372383200022 ER PT J AU Sumnall, M Peduzzi, A Fox, TR Wynne, RH Thomas, VA Cook, B AF Sumnall, Matthew Peduzzi, Alicia Fox, Thomas R. Wynne, Randolph H. Thomas, Valerie A. Cook, Bruce TI Assessing the transferability of statistical predictive models for leaf area index between two airborne discrete return LiDAR sensor designs within multiple intensely managed Loblolly pine forest locations in the south-eastern USA SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Loblolly pine; Forest management; LiDAR; Remote sensing; Leaf area index ID LASER-SCANNING DATA; WAVE-FORM LIDAR; VEGETATION INDEXES; LAI; EVAPOTRANSPIRATION; INVENTORY; DENSITY; METRICS; LEAVES AB Leaf area is an important forest structural variable which serves as the primary means of mass and energy exchange within vegetated ecosystems. The objective of the current study was to determine if leaf area index (LAI) could be estimated accurately and consistently in five intensively managed pine plantation forests using two multiple-return airborne LiDAR datasets. Field measurements of LAI were made using the LiCOR LAI2000 and LAI2200 instruments within 116 plots were established of varying size and within a variety of stand conditions (i.e. stand age, nutrient regime and stem density) in North Carolina and Virginia in 2008 and 2013. A number of common LiDAR return height and intensity distribution metrics were calculated (e.g. average return height), in addition to ten indices, with two additional variants, utilized in the surrounding literature which have been used to estimate LAI and fractional cover, were calculated from return heights and intensity, for each plot extent. Each of the indices was assessed for correlation with each other, and was used as independent variables in linear regression analysis with field LAI as the dependent variable. All LiDAR derived metrics were also entered into a forward stepwise linear regression. The results from each of the indices varied from an R-2 of 0.33 (S.E. 0.87) to 0.89 (S.E. 0.36). Those indices calculated using ratios of all returns produced the strongest correlations, such as the Above and Below Ratio Index (ABRI) and Laser Penetration Index 1 ( LPI1). The regression model produced from a combination of three metrics did not improve correlations greatly (R-2 0.90; S.E. 0.35). The results indicate that LAI can be predicted over a range of intensively managed pine plantation forest environments accurately when using different LiDAR sensor designs. Those indices which incorporated counts of specific return numbers (e.g. first returns) or return intensity correlated poorly with field measurements. There were disparities between the number of different types of returns and intensity values when comparing the results from two LiDAR sensors, indicating that predictive models developed using such metrics are not transferable between datasets with different acquisition parameters. Each of the indices were significantly correlated with one another, with one exception (LAI proxy), in particular those indices calculated from all returns, which indicates similarities in information content for those indices. It can then be argued that LiDAR indices have reached a similar stage in development to those calculated from optical-spectral sensors, but which offer a number of advantages, such as the reduction or removal of saturation issues in areas of high biomass. Published by Elsevier Inc. C1 [Sumnall, Matthew; Fox, Thomas R.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, 228 Cheatham Hall,Mail Code 0324, Blacksburg, VA 24061 USA. [Peduzzi, Alicia] US Forest Serv, USDA, 507,25th St, Ogden, UT 84401 USA. [Wynne, Randolph H.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, 319 Cheatham Hall,Mail Code 0324, Blacksburg, VA 24061 USA. [Thomas, Valerie A.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, 307A Cheatham Hall,Mail Code 0324, Blacksburg, VA 24061 USA. [Cook, Bruce] NASA, Goddard Space Flight Ctr, Biospher Sci Lab, Code 618, Greenbelt, MD 20771 USA. RP Sumnall, M (reprint author), Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, 228 Cheatham Hall,Mail Code 0324, Blacksburg, VA 24061 USA. FU Virginia Agricultural Experiment Station [VA-136623]; Program McIntire Stennis of the National Institute of Food and Agriculture, U.S. Department of Agriculture; Forest Productivity Cooperative; NASA FX This research was possible thanks to the support from the Forest Productivity Cooperative, the help in field data collection provided by Beth Stein and the assistance Alica Peduzzi, who provided the field data for validation of the 2008 LiDAR acquisition assessed in the project. We gratefully acknowledge the support and LiDAR data supplied by NASA, in addition to the support of the staff members of the Duke Forest teaching and research laboratory, the Northern Research Station Strategic Foresight Group of the North Carolina Forest service and the landowner Weyerhaeuser. Funding for this work was provided in part by the Virginia Agricultural Experiment Station (Project Number VA-136623) and the Program McIntire Stennis of the National Institute of Food and Agriculture, U.S. Department of Agriculture. NR 49 TC 2 Z9 3 U1 5 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD APR PY 2016 VL 176 BP 308 EP 319 DI 10.1016/j.rse.2016.02.012 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DG9DO UT WOS:000372383200024 ER PT J AU Rajao, DS Sandbulte, MR Gauger, PC Kitikoon, P Platt, R Roth, JA Perez, DR Loving, CL Vincent, AL AF Rajao, Daniela S. Sandbulte, Matthew R. Gauger, Phillip C. Kitikoon, Pravina Platt, Ratree Roth, James A. Perez, Daniel R. Loving, Crystal L. Vincent, Amy L. TI Heterologous challenge in the presence of maternally-derived antibodies results in vaccine-associated enhanced respiratory disease in weaned piglets SO VIROLOGY LA English DT Article DE Swine; Influenza; Vaccine associated enhanced respiratory disease; Maternally-derived antibodies ID SWINE INFLUENZA-VIRUS; IMMUNE-RESPONSES; PIGS; INFECTION; EFFICACY; TRANSMISSION; PROTECTION AB Control of influenza A virus (IAV) in pigs is done by vaccination of females to provide maternally-derived antibodies (MDA) through colostrum. Our aim was to evaluate if MDA interfere with IAV infection, clinical disease, and transmission in non-vaccinated piglets. In the first study, naive sows were vaccinated with H1N2-delta 1 whole inactivated virus (WIV) vaccine. In a follow-up study seropositive sows to 2009 pandemic H1N1 (H1N1pdm09) were boosted with H1N1pdm09 WIV or secondary experimental infection (EXP). MDA-positive pigs were challenged with homologous or heterologous virus, and MDA-negative control groups were included. WIV-MDA piglets were protected from homologous infection. However, piglets with WIV-derived MDA subsequently challenged with heterologous virus developed vaccine associated enhanced respiratory disease (VAERD), regardless of history of natural exposure in the sows. Our data indicates that although high titers of vaccine-derived MDA reduced homologous virus infection, transmission, and disease, MDA alone was sufficient to induce VAERD upon heterologous infection. Published by Elsevier Inc. C1 [Rajao, Daniela S.; Kitikoon, Pravina; Loving, Crystal L.; Vincent, Amy L.] ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. [Sandbulte, Matthew R.; Platt, Ratree; Roth, James A.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, 2180 Vet Med, Ames, IA 50011 USA. [Gauger, Phillip C.] Iowa State Univ, Vet Diagnost & Prod Anim Med, 2630 Vet Med, Ames, IA 50011 USA. [Perez, Daniel R.] Univ Georgia, Poultry Diagnost & Res Ctr, 953 Coll Stn Rd, Athens, GA 30602 USA. [Sandbulte, Matthew R.; Kitikoon, Pravina] Merck Anim Hlth, 35500W,91st St, De Soto, KS 66018 USA. RP Vincent, AL (reprint author), ARS, Virus & Prion Dis Livestock Res Unit, NADC, USDA, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov OI de Souza Rajao, Daniela/0000-0002-0772-0065 FU USDA-ARS; USDA-APHIS; National Institute of Allergy and Infectious Diseases [R21AI098079]; CNPq-Brazil FX We thank Michelle Harland, Gwen Nordholm, and Zahra Olson for technical assistance; Jason Huegel, Jason Crabtree, and Tyler Standley for assistance with animal studies; and Dr. Susan Brockmeier for assisting with bacterial screening. This study was supported by USDA-ARS, USDA-APHIS, and by National Institute of Allergy and Infectious Diseases contract R21AI098079. Dr. Rajao was a CNPq-Brazil fellowship recipient. NR 32 TC 1 Z9 1 U1 1 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD APR PY 2016 VL 491 BP 79 EP 88 DI 10.1016/j.virol.2016.01.015 PG 10 WC Virology SC Virology GA DH3CV UT WOS:000372666000009 PM 26874588 ER PT J AU Semmens, EO Domitrovich, J Conway, K Noonan, CW AF Semmens, Erin O. Domitrovich, Joseph Conway, Kathrene Noonan, Curtis W. TI A cross-sectional survey of occupational history as a wildland firefighter and health SO AMERICAN JOURNAL OF INDUSTRIAL MEDICINE LA English DT Article DE cardiovascular; musculoskeletal; health surveillance; wildland firefighters ID AIR-POLLUTION; OXIDATIVE STRESS; CANCER INCIDENCE; SMOKE EXPOSURE; CLIMATE-CHANGE; WOODSMOKE; DISEASE; RISKS AB BackgroundLittle is known regarding the impact of long-term wildland firefighting on health. Our objective was to investigate associations between duration of wildland firefighting experience and the prevalence of self-reported health outcomes. MethodsWe ascertained employment, health, and demographic information on 499 current wildland firefighters (WLFFs) via questionnaire. ResultsRelative to those with less than 10 years of experience as a WLFF, those with 10-19 years of experience had significantly greater odds of having ever been diagnosed with hypertension, as did those with 20 or more years of experience. Significant associations were observed for report of physician-diagnosed heart arrhythmia and previous knee surgery. ConclusionsWe observed significant links between a greater number of years as a WLFF and self-report of two subclinical cardiovascular risk factors as well as markers of musculoskeletal health. Additional studies are needed to determine if findings can be generalized to all WLFFs. (c) 2016 Wiley Periodicals, Inc. C1 [Semmens, Erin O.; Conway, Kathrene; Noonan, Curtis W.] Univ Montana, Ctr Environm Hlth Sci, Dept Biomed & Pharmaceut Sci, Missoula, MT 59812 USA. [Domitrovich, Joseph] USDA ARS, Missoula Technol & Dev Ctr, Missoula, MT USA. RP Semmens, EO (reprint author), Univ Montana, Dept Biomed Sci, Ctr Environm Hlth Sci, 32 Campus Dr, Missoula, MT 59812 USA. EM erin.semmens@umontana.edu FU USDA Forest Service Missoula Technology and Development Center [11-CR-11138200-009] FX This research was supported by the USDA Forest Service Missoula Technology and Development Center (11-CR-11138200-009). NR 29 TC 0 Z9 0 U1 6 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0271-3586 EI 1097-0274 J9 AM J IND MED JI Am. J. Ind. Med. PD APR PY 2016 VL 59 IS 4 BP 330 EP 335 DI 10.1002/ajim.22566 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DG7UP UT WOS:000372289500009 PM 26792645 ER PT J AU Henning, JA Gent, DH Twomey, MC Townsend, MS Pitra, NJ Matthews, PD AF Henning, J. A. Gent, D. H. Twomey, M. C. Townsend, M. S. Pitra, N. J. Matthews, P. D. TI Genotyping-by-sequencing of a bi-parental mapping population segregating for downy mildew resistance in hop (Humulus lupulus L.) SO EUPHYTICA LA English DT Article DE Downy mildew; Genotyping-by-sequencing; Genome; Humulus; Pseudoperonospora humuli; SNP ID GENETIC ARCHITECTURE; ASSOCIATION; REGISTRATION; WASHINGTON; DISCOVERY; TRAITS AB Downy mildew (caused by Pseudoperonospora humuli) causes significant losses in hop (Humulus lupulus L.) cone yield and quality, and potential crown death. Breeding for resistance has proved difficult presumably because of the highly quantitative nature of genetic control over expression. The objective of this study was to utilize multiple environments to ascertain downy mildew resistance and identify molecular markers linked to resistance. A widely segregating population, 'Teamaker' x USDA 21422M was inoculated and evaluated for downy mildew in field studies (Corvallis, OR and Yakima, WA) and a greenhouse study. Next generation sequencing using an Illumina HiSeq 2000 was performed on this population. Resulting reads were imported into UNEAK TASSEL 4.0 pipeline for processing and SNP-calls. Approximately 120,435 unfiltered SNP markers were identified and of these markers, 9081 high quality markers chosen for association analysis. Data analyses for field studies in OR and WA were performed separately using trait values averaged across blocks. Differential trait associated marker sets were identified across each physical environment with partial overlap between field environments. Mixed linear model analyses identified 39 markers (21 from OR, 10 from WA and eight overlapping) associated with response to downy mildew infection (p < 1 x 10(-4)). The most significant 17 markers were validated using high-resolution melting curve analysis and the resistant allele identified. Four SNP markers, along with the respective "resistant" alleles, were classified as highly selective for resistance (model adjusted R-2 = 0.437). Multiplexing studies are underway to develop a simple PCR tool for selecting downy mildew resistance. C1 [Henning, J. A.; Gent, D. H.; Twomey, M. C.] USDA ARS, Forage Seed Res Ctr Unit, 3450 SW Campus Way, Corvallis, OR 97331 USA. [Townsend, M. S.] Oregon State Univ, Dept Crops & Soil Sci, Corvallis, OR 97331 USA. [Pitra, N. J.; Matthews, P. D.] SS Steiner Inc, Hopsteiner, 655 Madison Ave, New York, NY 10065 USA. RP Henning, JA (reprint author), USDA ARS, Forage Seed Res Ctr Unit, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM john.henning@ars.usda.gov FU USDA-ARS CRIS [5358-21000-040-00D]; Hop Research Council FX Special thanks are extended to Edward Buckler (USDA-ARS, Cornell University) and his laboratory and members of Cornell's Institute for Genomic Diversity (Sharon Mitchell, Charlotte Acharya and Theresa Fulton) for all the helpful advice and suggestions that enabled this research to be successful. This research was funded through USDA-ARS CRIS project # 5358-21000-040-00D as well as funding support from the Hop Research Council (www.hopresearchcouncil.org). NR 40 TC 1 Z9 1 U1 6 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD APR PY 2016 VL 208 IS 3 BP 545 EP 559 DI 10.1007/s10681-015-1600-3 PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DG6DI UT WOS:000372172000010 ER PT J AU Robins, JG Lovatt, JA AF Robins, Joseph G. Lovatt, J. Alan TI Cultivar by environment effects of perennial ryegrass cultivars selected for high water soluble carbohydrates managed under differing precipitation levels SO EUPHYTICA LA English DT Article DE Drought; Genotype by environment Dinteraction; Irrigation; Lolium; Water ID DACTYLIS-GLOMERATA L.; 5 IRRIGATION LEVELS; DRY-MATTER YIELD; HERBAGE PRODUCTION; PERFORMANCE; ORCHARDGRASS; ACCESSIONS; QUALITY; GRASSES; DROUGHT AB Historic results of perennial ryegrass (Lolium perenne L.) breeding include improved disease resistance, biomass, and nutritional quality. Yet, lack of tolerance to water stress limits its wide use. Recent efforts to increase water soluble carbohydrate (WSC) content in perennial ryegrass may increase drought tolerance. Herein, we report results of a multi-year and location evaluation of differing precipitation/irrigations levels on genetic and genotype x environment interaction effects of various agronomic traits in perennial ryegrass. The study included two UK environments (Aberystwyth, Wales and Edinburgh, Scotland) and four USA environments located in Logan, UT and consisting in four supplemental irrigation levels in a line-source irrigation design. Data included herbage dry matter, dry matter digestibility, crude protein, and WSC, collected from 2011 to 2013. There were differences (P < 0.05) among the included cultivars for each phenotype when evaluated across environments. There was observed cultivar x environment interaction for each phenotype, which precluded the identification of the best cultivar over all environments. Additive main effect and multiplicative interaction modeling allowed for grouping of the individual environments to mega-environments. The analysis identified three mega-environments for each phenotype: (1) the UK environments, (2) the two highest Logan irrigation levels, and (3) the two lowest Logan irrigation levels. Mega-environments designations corresponded to water stress, temperature, and inherent geographic/climate differences between the environments. The evidence for the potential of increased WSC to improve drought tolerance under water stress was inconclusive, but high WSC cultivars also exhibited high herbage production under the imposed water stress. C1 [Robins, Joseph G.] USDA ARS, Dept Plants Soil & Climate, Forage & Range Res, Logan, UT USA. [Lovatt, J. Alan] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth SY23 3FG, Dyfed, Wales. RP Robins, JG (reprint author), USDA ARS, Dept Plants Soil & Climate, Forage & Range Res, Logan, UT USA. EM joseph.robins@ars.usda.gov NR 30 TC 0 Z9 0 U1 5 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD APR PY 2016 VL 208 IS 3 BP 571 EP 581 DI 10.1007/s10681-015-1607-9 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DG6DI UT WOS:000372172000012 ER PT J AU Kiss, L Holb, IJ Rossi, V Cadle-Davidson, L Jeger, MJ AF Kiss, Levente Holb, Imre J. Rossi, Vittorio Cadle-Davidson, Lance Jeger, Michael J. TI Foreword: Special issue on fungal grapevine diseases SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Editorial Material C1 [Kiss, Levente; Holb, Imre J.] Hungarian Acad Sci MTA ATK, Inst Plant Protect, Agr Res Ctr, POB 102, H-1525 Budapest, Hungary. [Holb, Imre J.] Univ Debrecen, Ctr Agr Sci & Engn, Fac Agron, H-4015 Debrecen, Hungary. [Rossi, Vittorio] Univ Cattolica Sacro Cuore, Dept Sutainable Crop Prod, Via E Parmense 84, I-29122 Piacenza, Italy. [Cadle-Davidson, Lance] USDA ARS, NYSAES, Grape Genet Res Unit, Geneva, NY 14456 USA. [Jeger, Michael J.] Univ London Imperial Coll Sci Technol & Med, Div Biol, Silwood Pk Campus, Ascot SL5 7PY, Berks, England. RP Kiss, L (reprint author), Hungarian Acad Sci MTA ATK, Inst Plant Protect, Agr Res Ctr, POB 102, H-1525 Budapest, Hungary. EM kiss.levente@agrar.mta.hu NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD APR PY 2016 VL 144 IS 4 SI SI BP 693 EP 694 DI 10.1007/s10658-015-0844-z PG 2 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DG7MN UT WOS:000372268500001 ER PT J AU Moyer, MM Londo, J Gadoury, DM Cadle-Davidson, L AF Moyer, Michelle M. Londo, Jason Gadoury, David M. Cadle-Davidson, Lance TI Cold Stress-Induced Disease Resistance (SIDR): indirect effects of low temperatures on host-pathogen interactions and disease progress in the grapevine powdery mildew pathosystem SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Cold shock; Stress physiology; Powdery mildew ID VITIS-VINIFERA L; UNCINULA-NECATOR; ERYSIPHE-NECATOR; FREEZING TOLERANCE; HIGHER-PLANTS; MODEL; ARABIDOPSIS; GROWTH; RESPONSES; LEAVES AB Erysiphe necator is an obligate biotroph capable of infecting three genera within the Vitaceae (Vitis, Parthenocissus, and Ampelopsis). The pathogen inhabits a niche unique to most powdery mildews, i.e., wholly external mycelial growth supported by haustoria within the subtending host epidermal cells. This growth habit coupled with its biotrophic reliance on the host makes E. necator sensitive to both direct effects of abiotic stresses on the pathogen and indirect abiotic effects via the host responses. Development of the pathogen during acute cold events (e.g., 1 h at 4 A degrees C) results in death of hyphal segments and a prolonged latency, an effect further increased by the development of ontogenic resistance as epidermal tissues of leaves and berries age. Acute cold events can also stress the host prior to the arrival of the pathogen, and thereby reduce susceptibility to infection via cold Stress-Induced Disease Resistance (SIDR), a recently described phenomenon. This effect requires approximately 24 h post-cold before maximal resistance effect occurs, and is also transient in that the effect diminishes to a basal level within 48 h after exposure. Although the phenotypic responses to cold SIDR may be similar to those observed on ontogenically-resistant leaves, the effects of cold SIDR and ontogenic resistance are additive. Sufficient tools are now available for investigating the mechanistic basis of cold SIDR. While pathosystems involving obligate biotrophs complicate research on direct and indirect environmental effects on the pathogen, this requisite interaction also creates interesting systems to understand how the condition of the host may influence subsequent disease development. At the population level, the effects of repeated cold events have profound effects on the nature of epidemic progress and implications for management of grape powdery mildew. The objective in this review is to summarize our current knowledge regarding the indirect and consequential effects of low temperature on the development of grapevine powdery mildew. C1 [Moyer, Michelle M.] Washington State Univ, Dept Hort Viticulture & Enol Program, IAREC, Prosser, WA USA. [Londo, Jason; Cadle-Davidson, Lance] USDA ARS, NYSAES, Grape Genet Res Unit, Geneva, NY USA. [Gadoury, David M.] Cornell Univ, NYSAES, Sect Plant Pathol & Plant Microbe Biol, Geneva, NY USA. RP Cadle-Davidson, L (reprint author), USDA ARS, NYSAES, Grape Genet Res Unit, Geneva, NY USA. EM Lance.CadleDavidson@ARS.USDA.GOV FU United States Department of Agriculture Viticulture Consortium-East; Pennsylvania Wine Marketing Board; New York Wine and Grape Foundation; New York Grape Production Research Fund; Kaplan Fund; American Society for Enology and Viticulture National and Eastern Sections FX The authors would like to acknowledge the contributions of students and technicians who helped to collect data related to acute cold responses, including: Bill Weldon, Kiersten Bekoscke, Mary Jean Welser, Michelle Schaub, Hema Kasinathan, Anna Nowogrodzki, Paige Appleton, Jackie Lillis, and Breanne Kisselstein. Funding was provided by the United States Department of Agriculture Viticulture Consortium-East, the Pennsylvania Wine Marketing Board, the New York Wine and Grape Foundation, the New York Grape Production Research Fund, the Kaplan Fund, and the American Society for Enology and Viticulture National and Eastern Sections. NR 68 TC 3 Z9 3 U1 11 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD APR PY 2016 VL 144 IS 4 SI SI BP 695 EP 705 DI 10.1007/s10658-015-0745-1 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DG7MN UT WOS:000372268500002 ER PT J AU Dimitrov, KM Ramey, AM Qiu, XT Bahl, J Afonso, CL AF Dimitrov, Kiril M. Ramey, Andrew M. Qiu, Xueting Bahl, Justin Afonso, Claudio L. TI Temporal, geographic, and host distribution of avian paramyxovirus 1 (Newcastle disease virus) SO INFECTION GENETICS AND EVOLUTION LA English DT Review DE Avian paramyxovirus 1 (APMV-1); Newcastle disease virus (NDV); Distribution; Host; Virulence; Genotype ID LIVE-BIRD MARKETS; COMPLETE GENOME SEQUENCE; DOUBLE-CRESTED CORMORANTS; COMPLETE NUCLEOTIDE-SEQUENCE; REVERSE-TRANSCRIPTION-PCR; FUSION PROTEIN GENE; WILD BIRDS; HEMAGGLUTININ-NEURAMINIDASE; PHYLOGENETIC CHARACTERIZATION; MOLECULAR EPIDEMIOLOGY AB Newcastle disease is caused by virulent forms of avian paramyxovirus of serotype 1 (APMV-1) and has global economic importance. The disease reached panzootic proportions within two decades after first being identified in 1926 in the United Kingdom and Indonesia and still remains endemic in many countries across the world. Here we review information on the host, temporal, and geographic distribution of APMV-1 genetic diversity based on the evolutionary systematics of the complete coding region of the fusion gene. Strains of APMV-1 are phylogenetically separated into two classes (class I and class II) and further classified into genotypes based on genetic differences. Class I viruses are genetically less diverse, generally present in wild waterfowl, and are of low virulence. Class II viruses are genetically and phenotypically more diverse, frequently isolated from poultry with occasional spillovers into wild birds, and exhibit a wider range of virulence. Waterfowl, cormorants, and pigeons are natural reservoirs of all APMV-1 pathotypes, except viscerotropic velogenic viruses for which natural reservoirs have not been identified. Genotypes I and II within class II include isolates of high and low virulence, the latter often being used as vaccines. Viruses of genotypes III and IX that emerged decades ago are now isolated rarely, but may be found in domestic and wild birds in China. Containing only virulent viruses and responsible for the majority of recent outbreaks in poultry and wild birds, viruses from genotypes V, VI, and VII, are highly mobile and have been isolated on different continents. Conversely, virulent viruses of genotypes XI (Madagascar), XIII (mainly Southwest Asia), XVI (North America) and XIV, XVII and XVIII (Africa) appear to have a more limited geographic distribution and have been isolated predominantly from poultry. Published by Elsevier B.V. C1 [Dimitrov, Kiril M.; Afonso, Claudio L.] ARS, USDA, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. [Ramey, Andrew M.] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. [Qiu, Xueting; Bahl, Justin] Univ Texas Houston, Sch Publ Hlth, 1200 Pressler St, Houston, TX 77030 USA. RP Dimitrov, KM; Afonso, CL (reprint author), ARS, USDA, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Kiril.Dimitrov@ars.usda.gov; Claudio.Afonso@ars.usda.gov OI Bahl, Justin/0000-0001-7572-4300; Ramey, Andrew/0000-0002-3601-8400 FU Department of State Biosecurity Engagement Program (BEP) [NDV 31063]; Defense Threat Reduction Agency Cooperative Biological Engagement Program, USDA/ARS [685/FRCALL 12-6-2-0005]; USDA CRIS [6612-32000-064-00D]; U.S. Geological Survey through Wildlife Program of the Ecosystems Mission area FX The authors gratefully acknowledge Patti Miller for her useful comments on the manuscript. This work was supported by the Department of State Biosecurity Engagement Program (BEP, NDV 31063), the Defense Threat Reduction Agency Cooperative Biological Engagement Program, USDA/ARS #685/FRCALL 12-6-2-0005, USDA CRIS 6612-32000-064-00D, and by the U.S. Geological Survey through the Wildlife Program of the Ecosystems Mission area. Any use of trade names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 153 TC 9 Z9 10 U1 6 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1567-1348 EI 1567-7257 J9 INFECT GENET EVOL JI Infect. Genet. Evol. PD APR PY 2016 VL 39 BP 22 EP 34 DI 10.1016/j.meegid.2016.01.008 PG 13 WC Infectious Diseases SC Infectious Diseases GA DG4DZ UT WOS:000372022700004 PM 26792710 ER PT J AU Gramaje, D Baumgartner, K Halleen, F Mostert, L Sosnowski, MR Urbez-Torres, JR Armengol, J AF Gramaje, D. Baumgartner, K. Halleen, F. Mostert, L. Sosnowski, M. R. Urbez-Torres, J. R. Armengol, J. TI Fungal trunk diseases: a problem beyond grapevines? SO PLANT PATHOLOGY LA English DT Letter ID WOOD DECAY; PATHOGENS; TREES C1 [Gramaje, D.] Univ la Rioja, CSIC, Gobierno La Rioja, Inst Ciencias Vid & Vino, Ctra LO-20 Salida 13, Finca La Grajera 26071, Logrono, Spain. [Baumgartner, K.] ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Halleen, F.] Agr Res Council Infruitec Nietvoorbij, Div Plant Protect, Private Bag X5026, ZA-7599 Stellenbosch, South Africa. [Halleen, F.; Mostert, L.] Univ Stellenbosch, Dept Plant Pathol, Private Bag X1, ZA-7600 Matieland, South Africa. [Sosnowski, M. R.] South Australian Res & Dev Inst, Adelaide, SA 5001, Australia. [Urbez-Torres, J. R.] Agr & Agri Food Canada, Pacific Agrifood Res Ctr, 4200 Highway 97,Box 5000, Summerland, BC V0H 1Z0, Canada. [Armengol, J.] Univ Politecn Valencia, Inst Agroforestal Mediterraneo, Camino Vera S-N, Valencia 46022, Spain. RP Gramaje, D (reprint author), Univ la Rioja, CSIC, Gobierno La Rioja, Inst Ciencias Vid & Vino, Ctra LO-20 Salida 13, Finca La Grajera 26071, Logrono, Spain. EM david.gramaje@icvv.es OI Gramaje, David/0000-0003-1755-3413 NR 11 TC 3 Z9 3 U1 2 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD APR PY 2016 VL 65 IS 3 BP 355 EP 356 DI 10.1111/ppa.12486 PG 2 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DG8ML UT WOS:000372338200001 ER PT J AU Edwards, MC Weiland, JJ Todd, J Stewart, LR Lu, SW AF Edwards, Michael C. Weiland, John J. Todd, Jane Stewart, Lucy R. Lu, Shunwen TI ORF43 of maize rayado fino virus is dispensable for systemic infection of maize and transmission by leafhoppers SO VIRUS GENES LA English DT Article DE Tymovirus; Marafivirus; Virus movement; Movement protein; Overlapping protein ID YELLOW MOSAIC-VIRUS; MOLECULAR CHARACTERIZATION; GENOME ORGANIZATION; GENUS MARAFIVIRUS; SEQUENCE; PROTEIN; EXPRESSION; IDENTIFICATION; TYMOVIRUSES; POINSETTIA AB Maize rayado fino virus (MRFV) possesses an open reading frame (ORF43) predicted to encode a 43 kDa protein (p43) that has been postulated to be a viral movement protein. Using a clone of MRFV (pMRFV-US) from which infectious RNA can be produced, point mutations were introduced to either prevent initiation from three potential AUG initiation codons near the 5'-end of ORF43 or prematurely terminate translation of ORF43. Inoculation of maize seed via vascular puncture inoculation (VPI) resulted in plants exhibiting symptoms typical of MRFV infection for all mutants tested. Furthermore, corn leafhoppers (Dalbulus maidis) transmitted the virus mutants to healthy plants at a frequency similar to that for wild-type MRFV-US. Viral RNA recovered from plants infected with mutants both prior to and after leafhopper transmission retained mutations blocking ORF43 expression. The results indicate that ORF43 of MRFV is dispensable for both systemic infection of maize and transmission by leafhoppers. C1 [Edwards, Michael C.; Weiland, John J.; Lu, Shunwen] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Todd, Jane; Stewart, Lucy R.] USDA, Agr Res Serv Corn, Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. RP Edwards, MC (reprint author), USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. EM michael.edwards@ars.usda.gov FU USDA-ARS CRIS project [3060-22000-048-00D, 5082-22000-013-00D] FX The authors would like to thank Andy Winter and Renee McClean for technical assistance. Funding for this work was provided by USDA-ARS CRIS project numbers 3060-22000-048-00D and 5082-22000-013-00D. NR 24 TC 0 Z9 0 U1 0 U2 2 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD APR PY 2016 VL 52 IS 2 BP 303 EP 307 DI 10.1007/s11262-016-1287-0 PG 5 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA DG6BI UT WOS:000372166800018 PM 26837893 ER PT J AU Sgolastra, F Arnan, X Pitts-Singer, TL Maini, S Kemp, WP Bosch, J AF Sgolastra, Fabio Arnan, Xavier Pitts-Singer, Theresa L. Maini, Stefano Kemp, William P. Bosch, Jordi TI Pre-wintering conditions and post-winter performance in a solitary bee: does diapause impose an energetic cost on reproductive success? SO ECOLOGICAL ENTOMOLOGY LA English DT Article DE Fecundity; fitness; life span; longevity; Osmia lignaria; ovary maturation; parental investment; sex ratio ID LIGNARIA-PROPINQUA CRESSON; QUEENS BOMBUS-TERRESTRIS; COLORADO POTATO BEETLE; LIFE-HISTORY TRAITS; RED MASON BEE; OSMIA-LIGNARIA; INSECT DIAPAUSE; BODY-SIZE; TRADE-OFF; RHAGOLETIS-POMONELLA AB 1. Diapause is a dynamic process of low metabolic activity that allows insects to survive periods of harsh conditions. Notwithstanding the lowered metabolism, and because diapausing insects have no access to food, diapause has an energetic cost that may affect post-diapause performance. 2. Previous studies on the solitary bee Osmia lignaria have shown that prolonged pre-wintering periods (the time during which individuals already in diapause remain at warm temperatures) are associated with elevated lipid consumption, fat body depletion, and body weight loss. The present study investigated whether prolonged pre-wintering also affects reproduction, i.e. whether the costs associated with diapause could have an effect on post-diapause performance in this species. 3. Females were exposed to a range of pre-wintering conditions, and ovary development and individual post-wintering performance were monitored throughout their adult life span. 4. No evidence of an effect of pre-wintering duration on post-diapause reproductive success was found. Expected differences in the timing of establishment were not observed because ovary maturation was, surprisingly, not arrested during pre-wintering. Prolonged pre-wintering duration did not result in decreased life span, probably because emerging females could rapidly replenish their metabolic reserves through feeding. However, there was a very strong effect of the duration of the pre-emergence period on the likelihood of nest establishment. 5. Longevity, the main factor determining fecundity in Osmia, is subjected to high levels of intrinsic variability, even among females of similar size exposed to identical conditions during development and nesting. C1 [Sgolastra, Fabio; Maini, Stefano] Univ Bologna, Dipartimento Sci Agr, Viale G Fanin 42, I-40127 Bologna, Italy. [Arnan, Xavier; Bosch, Jordi] Univ Autonoma Barcelona, CREAF, Bellaterra, Spain. [Arnan, Xavier] Univ Fed Pernambuco, Dept Bot, Recife, PE, Brazil. [Pitts-Singer, Theresa L.] USDA ARS, Pollinating Insects Biol Management & Systemat Re, Logan, UT USA. [Kemp, William P.] USDA ARS, Red River Valley Agr Res Ctr, Fargo, ND USA. RP Sgolastra, F (reprint author), Univ Bologna, Dipartimento Sci Agr, Viale G Fanin 42, I-40127 Bologna, Italy. EM fabio.sgolastra2@unibo.it RI Arnan, Xavier/N-7829-2013; OI Arnan, Xavier/0000-0002-9904-274X; Bosch , Jordi/0000-0002-8088-9457; Sgolastra, Fabio/0000-0002-2845-8297 FU University of Bologna FX G. Trostle and S. Kalaskar (USDA-ARS) provided invaluable assistance throughout the various phases of the experiments. F.S. was supported by a PhD scholarship from the University of Bologna. J.B., F.S., and W.P.K. conceived and designed the experiments. F.S. and T.P.S. performed the experiments. X.A., F.S., and S.M. were involved in data analysis. All authors contributed to the writing of the paper. We have no conflict of interest to declare. NR 62 TC 0 Z9 0 U1 12 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD APR PY 2016 VL 41 IS 2 BP 201 EP 210 DI 10.1111/een.12292 PG 10 WC Entomology SC Entomology GA DF9MP UT WOS:000371685600009 ER PT J AU Hanson, DL Loneragan, GH Brown, TR Nisbet, DJ Hume, ME Edrington, TS AF Hanson, D. L. Loneragan, G. H. Brown, T. R. Nisbet, D. J. Hume, M. E. Edrington, T. S. TI Evidence supporting vertical transmission of Salmonella in dairy cattle SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Epidemiology; Salmonella; zoonoses ID VIRAL DIARRHEA VIRUS; LYMPH-NODES; UNITED-STATES; HIGH-PLAINS; ENTERICA; CALVES; SEROTYPES; CARRIAGE; VACCINE; HARVEST AB We set out to investigate whether Salmonella enterica could be recovered from various tissues of viable neonatal calves immediately following parturition. Eleven samples were aseptically collected from each of 20 calves and consisted of both left and right subiliac and prescapular lymph nodes (LN), mesenteric LN, spleen and liver, as well as intestinal tissue (including luminal contents) from the small intestine, caecum, spiral colon and rectum. In addition, a faecal sample was collected from 19 of the dams. Salmonella was recovered from at least one sample from 10 of the 20 neonates. Across all calves, Salmonella was recovered from 12.7% of all samples and from LN in particular, Salmonella was recovered from 10.0%, 5.0%, and 5.0% of subiliac, prescapular, and mesenteric LN, respectively. Within calves, Salmonella was recovered from 0% to 73% of samples and across tissues, estimates of Salmonella prevalence were greatest in the caecum (30%) but was never recovered from the right pre-scapular LN. These data provide evidence of vertical transmission from a dam to her fetus such that viable calves are born already infected and thereby not requiring faecal-oral exposure for transmission. This new knowledge ought to challenge - or at least add to - existing paradigms of Salmonella transmission dynamics within cattle herds. C1 [Hanson, D. L.; Loneragan, G. H.] Texas Tech Univ, Int Ctr Food Ind Excellence, Lubbock, TX 79409 USA. [Brown, T. R.; Nisbet, D. J.; Hume, M. E.; Edrington, T. S.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX USA. RP Loneragan, GH (reprint author), Texas Tech Univ, Dept Anim & Food Sci, MS 42141, Lubbock, TX 79409 USA. EM Guy.Loneragan@ttu.edu FU United States Department of Agriculture, National Institutes of Food and Agriculture, National Integrated Food Safety Initiative [2011-51110-31081, 2010-51110-21083] FX This work was supported by the United States Department of Agriculture, National Institutes of Food and Agriculture, National Integrated Food Safety Initiative (award numbers 2011-51110-31081, 2010-51110-21083). NR 17 TC 2 Z9 2 U1 2 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD APR PY 2016 VL 144 IS 5 BP 962 EP 967 DI 10.1017/S0950268815002241 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DF9ZH UT WOS:000371720700007 PM 26419321 ER PT J AU Slesak, RA Harrington, TB Peter, DH DeBruler, DG Schoenholtz, SH Strahm, BD AF Slesak, Robert A. Harrington, Timothy B. Peter, David H. DeBruler, Daniel G. Schoenholtz, Stephen H. Strahm, Brian D. TI Effects of intensive management practices on 10-year Douglas-fir growth, soil nutrient pools, and vegetation communities in the Pacific Northwest, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest biomass harvest; Organic matter manipulation; Vegetation dynamics; Long term soil productivity; Early successional ID LOBLOLLY-PINE PLANTATIONS; COMPETING-VEGETATION; LOGGING-DEBRIS; SUSTAINED PRODUCTIVITY; PTERIDIUM-AQUILINUM; FOREST PLANTATIONS; TEMPERATE FORESTS; BIOMASS REMOVAL; CARBON STORAGE; HARVEST AB Intensive management practices are commonly used to increase fiber production from forests, but potential tradeoffs with maintenance of long-term productivity and early successional biodiversity have yet to be quantified. We assessed soil and vegetation responses in replicated manipulations of logging debris (LD; either retained or removed) and competing vegetation control (VC; either initial or sustained annually for 5 years) for 10 years at two Douglas-fir sites that contrasted strongly in availability of soil nutrients and water. We evaluated (1) survival and growth of Douglas-fir to determine short-term effectiveness for fiber production, (2) change in soil C and nutrient pools as an indicator of longer-term effects of treatments on soil quality and ecosystem production, and (3) vegetation composition and cover for treatment effects on early successional biodiversity. Annual VC caused large increases in Douglas-fir growth at both sites, but increased survival only at the lower-productivity site. In most instances and regardless of site or treatment, soil C and nutrient pools increased following harvesting, but the increases were generally larger with lower intensity practices (LD retained and initial VC). Effects of LD were small and inconsistent at the higher productivity site, but LD retained increased Douglas-fir survival and growth and soil nutrient pools at the lower productivity site. Species diversity was reduced at both sites with annual VC because of increased Douglas-fir cover, but the magnitude was greater and the timing was earlier at the higher quality site where plant communities in all treatments had converged by year 10. Annual VC can be used to increase growth of planted Douglas-fir while maintaining soil nutrient pools for sustained ecosystem productivity, but a concurrent decrease in early successional diversity will occur with impacts increasing with site quality. Logging debris retention can have positive benefits to Douglas fir growth and soil nutrient pools, particularly at lower quality sites. Our results demonstrate a need for careful consideration of site quality to ensure that objectives are realized with regards to fiber production and maintenance of soil productivity and biodiversity with intensive forest management. (C) 2016 Elsevier B.V. All rights reserved. C1 [Slesak, Robert A.] Minnesota Forest Resources Council, St Paul, MN 55108 USA. [Harrington, Timothy B.; Peter, David H.] USDA Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. [DeBruler, Daniel G.; Strahm, Brian D.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Schoenholtz, Stephen H.] Virginia Tech, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. RP Slesak, RA (reprint author), 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. EM raslesak@umn.edu FU USDA Forest Service Pacific Northwest Research Station; Port Blakely Tree Farms LLC; Virginia Tech; Green Diamond Resource Company; Randall Greggs; Mike Warjone FX This is a product of the Sustainable Forestry component of Agenda 2020, a joint effort of the USDA Forest Service Research and Development and the American Forest and Paper Association. Funds were provided by the USDA Forest Service Pacific Northwest Research Station, Virginia Tech, Green Diamond Resource Company, and Port Blakely Tree Farms LLC. The authors are especially grateful to Randall Greggs and Mike Warjone for providing critical financial and logistical support that made this study possible. Special thanks to J. Dollins, D. Stephens, B. Korman, D. Livada, and W. Devine for assisting with field and laboratory measurements. NR 73 TC 1 Z9 1 U1 7 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2016 VL 365 BP 22 EP 33 DI 10.1016/j.foreco.2016.01.019 PG 12 WC Forestry SC Forestry GA DF9AJ UT WOS:000371652300003 ER PT J AU Gomez-Casanovas, N Hudiburg, TW Bernacchi, CJ Parton, WJ Delucia, EH AF Gomez-Casanovas, Nuria Hudiburg, Tara W. Bernacchi, Carl J. Parton, William J. Delucia, Evan H. TI Nitrogen deposition and greenhouse gas emissions from grasslands: uncertainties and future directions SO GLOBAL CHANGE BIOLOGY LA English DT Article DE CH; (4); grassland; methane; N2O; net ecosystem CO2 exchange; net ecosystem productivity; nitrogen deposition; nitrogen fertilization; nitrous oxide; uncertainty ID SOIL CARBON SEQUESTRATION; CLIMATE-CHANGE; TERRESTRIAL ECOSYSTEMS; PRIMARY PRODUCTIVITY; PRAIRIE GRASSLANDS; NONLINEAR RESPONSE; REACTIVE NITROGEN; METAANALYSIS; ENRICHMENT; CYCLE AB Increases in atmospheric nitrogen deposition (N-dep) can strongly affect the greenhouse gas (GHG; CO2, CH4, and N2O) sink capacity of grasslands as well as other terrestrial ecosystems. Robust predictions of the net GHG sink strength of grasslands depend on how experimental N loads compare to projected N-dep rates, and how accurately the relationship between GHG fluxes and N-dep is characterized. A literature review revealed that the vast majority of experimental N loads were higher than levels these ecosystems are predicted to experience in the future. Using a process-based biogeochemical model, we predicted that low levels of N-dep either enhanced or reduced the net GHG sink strength of most grasslands, but as experimental N loads continued to increase, grasslands transitioned to a N saturation-decline stage, where the sensitivity of GHG exchange to further increases in N-dep declined. Most published studies represented treatments well into the N saturation-decline stage. Our model results predict that the responses of GHG fluxes to N are highly nonlinear and that the N saturation thresholds for GHGs varied greatly among grasslands and with fire management. We predict that during the 21st century some grasslands will be in the N limitation stage where others will transition into the N saturation-decline stage. The linear relationship between GHG sink strength and N load assumed by most studies can overestimate or underestimate predictions of the net GHG sink strength of grasslands depending on their N baseline status. The next generation of global change experiments should be designed at multiple N loads consistent with future N-dep rates to improve our empirical understanding and predictive ability. C1 [Gomez-Casanovas, Nuria; Bernacchi, Carl J.; Delucia, Evan H.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Gomez-Casanovas, Nuria; Delucia, Evan H.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Gomez-Casanovas, Nuria; Bernacchi, Carl J.; Delucia, Evan H.] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. [Hudiburg, Tara W.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. [Bernacchi, Carl J.] USDA, Agr Res Serv, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Parton, William J.] Colorado State Univ, Natl Renewable Ecol Lab, Ft Collins, CO 80523 USA. RP Delucia, EH (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA.; Delucia, EH (reprint author), Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA.; Delucia, EH (reprint author), Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. EM delucia@life.illinois.edu FU Energy Biosciences Institute (EBI; Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA); US Department of Energy SunGrant Program [DE-FG36-08GO88073] FX This project was funded by the Energy Biosciences Institute (EBI; Univ. of Illinois at Urbana-Champaign, Urbana, IL, USA) and the US Department of Energy SunGrant Program (DE-FG36-08GO88073). We thank Jiwoong Hong for assistance in model parameterization. We are also grateful to Ms. Elena Blanc-Betes for helpful comments provided on the manuscript. NR 66 TC 2 Z9 2 U1 31 U2 110 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD APR PY 2016 VL 22 IS 4 BP 1348 EP 1360 DI 10.1111/gcb.13187 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DF7CN UT WOS:000371515300003 PM 26661794 ER PT J AU Schwalm, D Epps, CW Rodhouse, TJ Monahan, WB Castillo, JA Ray, C Jeffress, MR AF Schwalm, Donelle Epps, Clinton W. Rodhouse, Thomas J. Monahan, William B. Castillo, Jessica A. Ray, Chris Jeffress, Mackenzie R. TI Habitat availability and gene flow influence diverging local population trajectories under scenarios of climate change: a place-based approach SO GLOBAL CHANGE BIOLOGY LA English DT Article DE American pika; functional connectivity; genetic neighborhood; National Parks; Ochotona princeps; Random Forest; realized niche; species distribution modeling ID PIKA OCHOTONA-PRINCEPS; DISTRIBUTION MODELS IMPLICATIONS; WESTERN GREAT-BASIN; AMERICAN PIKA; SPECIES DISTRIBUTION; CONSERVATION; PATTERNS; SHIFTS; DISTRIBUTIONS; CONNECTIVITY AB Ecological niche theory holds that species distributions are shaped by a large and complex suite of interacting factors. Species distribution models (SDMs) are increasingly used to describe species' niches and predict the effects of future environmental change, including climate change. Currently, SDMs often fail to capture the complexity of species' niches, resulting in predictions that are generally limited to climate-occupancy interactions. Here, we explore the potential impact of climate change on the American pika using a replicated place-based approach that incorporates climate, gene flow, habitat configuration, and microhabitat complexity into SDMs. Using contemporary presence-absence data from occupancy surveys, genetic data to infer connectivity between habitat patches, and 21 environmental niche variables, we built separate SDMs for pika populations inhabiting eight US National Park Service units representing the habitat and climatic breadth of the species across the western United States. We then predicted occurrence probability under current (1981-2010) and three future time periods (out to 2100). Occurrence probabilities and the relative importance of predictor variables varied widely among study areas, revealing important local-scale differences in the realized niche of the American pika. This variation resulted in diverse and - in some cases - highly divergent future potential occupancy patterns for pikas, ranging from complete extirpation in some study areas to stable occupancy patterns in others. Habitat composition and connectivity, which are rarely incorporated in SDM projections, were influential in predicting pika occupancy in all study areas and frequently outranked climate variables. Our findings illustrate the importance of a place-based approach to species distribution modeling that includes fine-scale factors when assessing current and future climate impacts on species' distributions, especially when predictions are intended to manage and conserve species of concern within individual protected areas. C1 [Schwalm, Donelle; Epps, Clinton W.; Castillo, Jessica A.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Rodhouse, Thomas J.] Upper Columbia Basin Network, Natl Pk Serv, 650 SW Columbia St,Suite 7250, Montana, OR 97702 USA. [Monahan, William B.] USDA Forest Serv, Forest Hlth Technol Enterprise Team, 2150 Ctr Ave Bldg A,Suite 331, Ft Collins, CO 80526 USA. [Ray, Chris] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. [Jeffress, Mackenzie R.] Nevada Dept Wildlife, 60 Youth Ctr Rd, Elko, NV 89801 USA. [Monahan, William B.] US Forest Serv Forest Hlth Protect, State & Private Forestry, Washington Off, Forest Hlth Technol Enterprise Team, Ft Collins, CO 80525 USA. RP Schwalm, D (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. EM doni.schwalm@oregonstate.edu OI RAY, CHRIS/0000-0002-7963-9637; Rodhouse, Thomas/0000-0001-5953-9113 FU NASA-NPS Landscape Climate Change Vulnerability Project (NASA Applied Sciences) [10-BIOCLIM10-0034]; US National Park Service Climate Change Response Program (PMIS) [163377]; USGS Park-Oriented Biological Support (POBS) Program [G11AC20397]; Thomas G. Scott Publications Fund FX L. Garrett, M. Britten, S. Wolff, and K. Gunther were instrumental in project design and implementation; W. Schweiger and R. Anthony contributed to project design. The Crater Lake Science and Learning Center and the parks involved in the study provided additional personnel, material, and logistical support. The NEX-DCP30 dataset was prepared by the Climate Analytics Group and NASA Ames Research Center using the NASA Earth Exchange, and distributed by the NASA Center for Climate Simulation (NCCS). We thank F. Melton, A. Guzman, and the NASA-NPS Landscape Climate Change Vulnerability Project (LCC-VP; NASA Applied Sciences 10-BIOCLIM10-0034) for assistance with NEX-DCP30 data. T. Creech, R. Crowhurst, K. Moriarty and R. Spaan provided comments on an earlier version of this manuscript, and M. Lonneker provided GIS support. Funding was provided by the US National Park Service Climate Change Response Program (PMIS # 163377), the USGS Park-Oriented Biological Support (POBS) Program (grant # G11AC20397), and the Thomas G. Scott Publications Fund. We thank Philippe Henry and one anonymous reviewer for their helpful comments on an earlier version of this manuscript. NR 69 TC 4 Z9 4 U1 14 U2 45 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD APR PY 2016 VL 22 IS 4 BP 1572 EP 1584 DI 10.1111/gcb.13189 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DF7CN UT WOS:000371515300021 PM 26667878 ER PT J AU Burkle, LA Runyon, JB AF Burkle, Laura A. Runyon, Justin B. TI Drought and leaf herbivory influence floral volatiles and pollinator attraction SO GLOBAL CHANGE BIOLOGY LA English DT Article DE Campanula rotundifolia; climate change; floral display; floral scent; Heterotheca villosa; Phacelia hastata; plant-pollinator interactions; pollinator community; Potentilla recta; volatile organic compounds ID INDUCED PLANT VOLATILES; CLIMATE-CHANGE; GLOBAL CHANGE; INSECT HERBIVORE; MANDUCA-SEXTA; ECOLOGY; IMPACTS; STRESS; CONSEQUENCES; REPRODUCTION AB The effects of climate change on species interactions are poorly understood. Investigating the mechanisms by which species interactions may shift under altered environmental conditions will help form a more predictive understanding of such shifts. In particular, components of climate change have the potential to strongly influence floral volatile organic compounds (VOCs) and, in turn, plant-pollinator interactions. In this study, we experimentally manipulated drought and herbivory for four forb species to determine effects of these treatments and their interactions on (1) visual plant traits traditionally associated with pollinator attraction, (2) floral VOCs, and (3) the visitation rates and community composition of pollinators. For all forbs tested, experimental drought universally reduced flower size and floral display, but there were species-specific effects of drought on volatile emissions per flower, the composition of compounds produced, and subsequent pollinator visitation rates. Moreover, the community of pollinating visitors was influenced by drought across forb species (i.e. some pollinator species were deterred by drought while others were attracted). Together, these results indicate that VOCs may provide more nuanced information to potential floral visitors and may be relatively more important than visual traits for pollinator attraction, particularly under shifting environmental conditions. C1 [Burkle, Laura A.] Montana State Univ, Dept Ecol, Bozeman, MT 59717 USA. [Runyon, Justin B.] US Forest Serv, USDA, Rocky Mt Res Stn, 1648 S 7th Ave, Bozeman, MT 59717 USA. RP Burkle, LA (reprint author), Montana State Univ, Dept Ecol, Bozeman, MT 59717 USA. EM laura.burkle@montana.edu FU US Forest Service - Rocky Mountain Research Station, Montana State University; Hughes Summer Undergraduate Research Program - Howard Hughes Medical Institute FX We thank C. Delphia, P. Mitchell, J. Haas, and E. Reese for help in lab and field. This work was supported by funding from the US Forest Service - Rocky Mountain Research Station, Montana State University, and The Hughes Summer Undergraduate Research Program - Howard Hughes Medical Institute. NR 56 TC 3 Z9 3 U1 32 U2 76 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD APR PY 2016 VL 22 IS 4 BP 1644 EP 1654 DI 10.1111/gcb.13149 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DF7CN UT WOS:000371515300026 PM 26546275 ER PT J AU Spindel, JE Begum, H Akdemir, D Collard, B Redona, E Jannink, JL McCouch, S AF Spindel, J. E. Begum, H. Akdemir, D. Collard, B. Redona, E. Jannink, J-L McCouch, S. TI Genome-wide prediction models that incorporate de novo GWAS are a powerful new tool for tropical rice improvement SO HEREDITY LA English DT Article ID MARKER-ASSISTED SELECTION; ORYZA-SATIVA L.; COMPLEX TRAITS; MAIZE POPULATIONS; MOLECULAR MARKERS; CROP IMPROVEMENT; GRAIN-YIELD; ASSOCIATION; WHEAT; REGRESSION AB To address the multiple challenges to food security posed by global climate change, population growth and rising incomes, plant breeders are developing new crop varieties that can enhance both agricultural productivity and environmental sustainability. Current breeding practices, however, are unable to keep pace with demand. Genomic selection (GS) is a new technique that helps accelerate the rate of genetic gain in breeding by using whole-genome data to predict the breeding value of offspring. Here, we describe a new GS model that combines RR-BLUP with markers fit as fixed effects selected from the results of a genome-wide-association study (GWAS) on the RR-BLUP training data. We term this model GS + de novo GWAS. In a breeding population of tropical rice, GS + de novo GWAS outperformed six other models for a variety of traits and in multiple environments. On the basis of these results, we propose an extended, two-part breeding design that can be used to efficiently integrate novel variation into elite breeding populations, thus expanding genetic diversity and enhancing the potential for sustainable productivity gains. C1 [Spindel, J. E.; Akdemir, D.; Jannink, J-L; McCouch, S.] Cornell Univ, Dept Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY USA. [Begum, H.; Collard, B.; Redona, E.] Int Rice Res Inst, Dept Plant Breeding Genet & Biotechnol, Los Banos, Philippines. [Jannink, J-L] USDA ARS, North Atlantic Ares, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Begum, H.] Bangladesh Rice Res Inst, Gazipur 1701, Bangladesh. [Redona, E.] Delta Res & Extens Ctr, 82 Stoneville Rd,POB 197, Stoneville, MS 38776 USA. RP McCouch, S (reprint author), Cornell Univ, Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY 14853 USA. EM srm4@cornell.edu RI Spindel, Jennifer/F-1373-2017 FU NSF [PGRP 102655]; Organization for Women in Science for the Developing World (OWSD); Japan Government via Japan Rice Breeding Project at IRRI FX We thank Francisco Agosto-Perez, Genevieve DeClerck and Anthony Greenberg for statistical and computational support, Sandra Harrington and Gen Onishi for greenhouse support, and Charlotte Acharya and Wenyan Zhu for assistance with genotyping-by-sequencing library preps. Funding provided by NSF PGRP 102655 to SMC, a NSF graduate research fellowship to JES, the Organization for Women in Science for the Developing World (OWSD) for the PhD scholarship for HB and the Japan Government via the Japan Rice Breeding Project at IRRI. All data sets are publicly available at http://www.ricediversity.org. NR 55 TC 7 Z9 7 U1 6 U2 41 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD APR PY 2016 VL 116 IS 4 BP 395 EP 408 DI 10.1038/hdy.2015.113 PG 14 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA DG2LJ UT WOS:000371898300007 PM 26860200 ER PT J AU Cook, SC Eubanks, MD Gold, RE Behmer, ST AF Cook, Steven C. Eubanks, Micky D. Gold, Roger E. Behmer, Spencer T. TI Summer and fall ants have different physiological responses to food macronutrient content SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Carbohydrate; Lipids; Nutrient regulation; Protein; Solenopsis invicta ID SOLENOPSIS-INVICTA BUREN; DIVISION-OF-LABOR; FIRE ANT; LIFE-SPAN; BIOLOGICAL CLOCKS; METABOLIC-RATE; NATIVE ANTS; COLONIES; REPRODUCTION; HYMENOPTERA AB Seasonally, long-lived animals exhibit changes in behavior and physiology in response to shifts in environmental conditions, including food abundance and nutritional quality. Ants are long-lived arthropods that, at the colony level, experience such seasonal shifts in their food resources. Previously we reported summer- and fall-collected ants practiced distinct food collection behavior and nutrient intake regulation strategies in response to variable food protein and carbohydrate content, despite being reared in the lab under identical environmental conditions and dietary regimes. Seasonally distinct responses were observed for both no-choice and choice dietary experiments. Using data from these same experiments, our objective here is to examine colony and individual-level physiological traits, colony mortality and growth, food processing, and worker lipid mass, and how these traits change in response to variable food protein-carbohydrate content. For both experiments we found that seasonality per se exerted strong effects on colony and individual level traits. Colonies collected in the summer maintained total worker mass despite high mortality. In contrast, colonies collected in the fall lived longer, and accumulated lipids, including when reared on protein-biased diets. Food macronutrient content had mainly transient effects on physiological responses. Extremes in food carbohydrate content however, elicited a compensatory response in summer worker ants, which processed more protein-biased foods and contained elevated lipid levels. Our study, combined with our previously published work, strongly suggests that underlying physiological phenotypes driving behaviors of summer and fall ants are likely fixed seasonally, and change circannually. Published by Elsevier Ltd. C1 [Cook, Steven C.; Eubanks, Micky D.; Gold, Roger E.; Behmer, Spencer T.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Cook, Steven C.] USDA ARS, Beltsville Agr Res Ctr, Bee Res Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Cook, SC (reprint author), USDA ARS, Beltsville Agr Res Ctr, Bee Res Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM steven.cook@ars.usda.gov NR 67 TC 0 Z9 0 U1 10 U2 23 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD APR PY 2016 VL 87 BP 35 EP 44 DI 10.1016/j.jinsphys.2016.02.001 PG 10 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA DG1OE UT WOS:000371836600005 PM 26860359 ER PT J AU Wang, J Fu, LY Koganti, PP Wang, L Hand, JM Ma, H Yao, JB AF Wang, Jian Fu, Liyuan Koganti, Prasanthi P. Wang, Lei Hand, Jacqelyn M. Ma, Hao Yao, Jianbo TI Identification and Functional Prediction of Large Intergenic Noncoding RNAs (lincRNAs) in Rainbow Trout (Oncorhynchus mykiss) SO MARINE BIOTECHNOLOGY LA English DT Article DE Co-expression network; LncRNA; LincRNA; Rainbow trout ID FAMILIES DATABASE; GENE-EXPRESSION; CHROMATIN; SEQ; DIFFERENTIATION; ANNOTATION; REVEALS; PROTEIN; CELLS; QUANTIFICATION AB Long noncoding RNAs (lncRNAs) have been recognized in recent years as key regulators of diverse cellular processes. Genome-wide large-scale projects have uncovered thousands of lncRNAs in many model organisms. Large intergenic noncoding RNAs (lincRNAs) are lncRNAs that are transcribed from intergenic regions of genomes. To date, no lincRNAs in non-model teleost fish have been reported. In this report, we present the first reference catalog of 9674 rainbow trout lincRNAs based on analysis of RNA-Seq data from 15 tissues. Systematic analysis revealed that lincRNAs in rainbow trout share many characteristics with those in other mammalian species. They are shorter and lower in exon number and expression level compared with protein-coding genes. They show tissue-specific expression pattern and are typically co-expressed with their neighboring genes. Co-expression network analysis suggested that many lincRNAs are associated with immune response, muscle differentiation, and neural development. The study provides an opportunity for future experimental and computational studies to uncover the functions of lincRNAs in rainbow trout. C1 [Wang, Jian; Fu, Liyuan; Koganti, Prasanthi P.; Wang, Lei; Hand, Jacqelyn M.; Yao, Jianbo] W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. [Ma, Hao] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Yao, JB (reprint author), W Virginia Univ, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. EM jianbo.yao@mail.wvu.edu FU USDA ARS [58-1930-0-059] FX This study was supported by the USDA ARS Cooperative Agreement No. 58-1930-0-059. It is published with the approval of the director of the West Virginia Agricultural and Forestry Experiment Station as scientific paper No. 3268. NR 59 TC 3 Z9 3 U1 2 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-2228 EI 1436-2236 J9 MAR BIOTECHNOL JI Mar. Biotechnol. PD APR PY 2016 VL 18 IS 2 BP 271 EP 282 DI 10.1007/s10126-016-9689-5 PG 12 WC Biotechnology & Applied Microbiology; Marine & Freshwater Biology SC Biotechnology & Applied Microbiology; Marine & Freshwater Biology GA DG1FA UT WOS:000371809800011 PM 26864089 ER PT J AU Wallingford, AK Hesler, SP Cha, DH Loeb, GM AF Wallingford, Anna K. Hesler, Stephen P. Cha, Dong H. Loeb, Gregory M. TI Behavioral response of spotted-wing drosophila, Drosophila suzukii Matsumura, to aversive odors and a potential oviposition deterrent in the field SO PEST MANAGEMENT SCIENCE LA English DT Article DE Drosophila suzukii; oviposition deterrent; geosmin; 1-octen-3-ol ID INTEGRATED PEST-MANAGEMENT; HOST MARKING PHEROMONE; DIPTERA DROSOPHILIDAE; FRUIT-FLY; VOLATILE METABOLITES; SYNTHETIC MEDIA; UNITED-STATES; BERRY CROPS; TRAP DESIGN; MELANOGASTER AB BACKGROUNDDrosophilia suzukii Matsumura is an invasive pest insect that lays its eggs in the fruit of several commercially grown crops. An effective oviposition deterrent could contribute to its management. Repellant odors were evaluated in the laboratory and in the field. RESULTSGeosmin and 1-octen-3-ol were found to be aversive to seven-day-old female D. suzukii at concentrations of 10(-1) and 10(-2) in laboratory choice tests. Field experiments found that fewer eggs were observed in fruit on the day of harvest and fewer adult D. suzukii were reared from fruit associated with 1-octen-3-ol odors than control fruit in cultivated red raspberry. CONCLUSIONGeosmin and 1-octen-3-ol induce aversive behaviors in Drosophila suzukii and are potential oviposition deterrents for its management in fruit crops. (c) 2015 Society of Chemical Industry C1 [Wallingford, Anna K.; Hesler, Stephen P.; Cha, Dong H.; Loeb, Gregory M.] Cornell Univ, New York Agr Expt Stn, 630 W North St, Geneva, NY 14456 USA. [Cha, Dong H.] USDA ARS, Yakima Agr Res Lab, Wapato, WA USA. RP Wallingford, AK (reprint author), Cornell Univ, New York Agr Expt Stn, 630 W North St, Geneva, NY 14456 USA. EM akw52@cornell.edu FU Cornell University's New York State Agricultural Experiment Station Federal Formula Funds [2012-13-195]; NYS Department of Agriculture and Markets Specialty Crops Block Grant Program [C200783]; NYS Farm Viability Initiative [FOC 14 001] FX We would like to thank Dr Courtney Weber for the use of his mixed variety planting of fall-bearing raspberry at NYSAES for our field trials, and Gabrielle Brind'Amour and Amanda Schenk for assistance with laboratory bioassays. Thanks to Erika Mudrak for her assistance with statistical analysis. Justin Renkema, Paul Robbins, Wee Yee and two anonymous reviewers provided insightful suggestions for improvement to the manuscript. This research was supported in part by funding from Cornell University's New York State Agricultural Experiment Station Federal Formula Funds (Project No. 2012-13-195), the NYS Department of Agriculture and Markets Specialty Crops Block Grant Program (No. C200783) and the NYS Farm Viability Initiative (No. FOC 14 001) to GL. NR 64 TC 4 Z9 4 U1 12 U2 69 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD APR PY 2016 VL 72 IS 4 BP 701 EP 706 DI 10.1002/ps.4040 PG 6 WC Agronomy; Entomology SC Agriculture; Entomology GA DF7NH UT WOS:000371543900007 PM 25973596 ER PT J AU Witmer, GW Snow, NP Moulton, RS AF Witmer, Gary W. Snow, Nathan P. Moulton, Rachael S. TI Retention time of chlorophacinone in black-tailed prairie dogs informs secondary hazards from a prairie dog rodenticide bait SO PEST MANAGEMENT SCIENCE LA English DT Article DE anticoagulant; poisoning; rodenticide; rodent damage; secondary hazards; toxicant ID ANTICOAGULANT RODENTICIDES; ANIMAL-TISSUES; BROMADIOLONE; TOXICITY; RESIDUES; COLONY AB BACKGROUNDSecondary toxicity in mammals and birds that consume animals containing residues of anticoagulant rodenticides represents a persistent conflict between conservation, agriculture and environmental contamination. Chlorophacinone residues in black-tailed prairie dogs (Cynomys ludovicianus) represent a secondary exposure hazard to predatory and scavenging avian and mammalian species in the Central Plains of the United States, especially considering efforts to re-establish black-footed ferrets (Mustela nigripes). Rozol((R)) Prairie Dog Bait (chlorophacinone 0.005%) is registered to control black-tailed prairie dogs in ten states throughout the midwestern and western United States. RESULTSWe fed Rozol Prairie Dog Bait to captive black-tailed prairie dogs for 2 days and analyzed their livers and whole bodies (without livers) for chlorophacinone residue on days 3, 5, 7, 9, 11, 14, 18 and 27 post-exposure. We found the greatest levels of residues in livers ( = 5.499 mg kg(-1)) and whole bodies ( = 1.281 mg kg(-1)) on day 3. Residues in both tissues declined rapidly over time, with estimated half-lives of approximately 6 days post-exposure. However, a risk assessment of secondary toxicity to non-target mammals indicated acute risks for mammalian species up to 27 days post-exposure and negligible risks for birds. CONCLUSIONThe results suggest that the greatest risk of secondary toxicity occurs 14 days post-application of Rozol Prairie Dog Bait and declines thereafter. This corresponds to the time when chlorophacinone residues are high, and prairie dogs exhibit signs of intoxication and are perhaps most susceptible to predation and scavenging. These results confirm that Rozol Prairie Dog Bait should not be used in areas where black-footed ferrets or other sensitive species occur. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Witmer, Gary W.; Snow, Nathan P.; Moulton, Rachael S.] Natl Wildlife Res Ctr, USDA, APHIS, WS, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Witmer, GW (reprint author), Natl Wildlife Res Ctr, USDA, APHIS, WS, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Gary.W.Witmer@aphis.usda.gov FU United States Fish and Wildlife Service FX We thank Buckley Air Force Base for providing us access to capture prairie dogs. We also thank the NWRC Analytical Chemistry Unit for analyzing the tissue samples. This project was funded by the United States Fish and Wildlife Service. The study was conducted under IACUC-approved Study Protocol QA-754. Mention of a commercial product or company does not represent an endorsement by the US government. We thank anonymous reviewers for their comments on this manuscript. NR 34 TC 0 Z9 0 U1 3 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD APR PY 2016 VL 72 IS 4 BP 725 EP 730 DI 10.1002/ps.4045 PG 6 WC Agronomy; Entomology SC Agriculture; Entomology GA DF7NH UT WOS:000371543900010 PM 25997570 ER PT J AU Anderson, RL AF Anderson, Randy L. TI Converting perennial legumes to organic cropland without tillage SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE corn; fall mowing; no-till; red clover; stand density; weed density ID MEDICAGO-SATIVA; ROTATION; CORN; AGRICULTURE; CROPS AB Organic producers are interested in developing a no-till system for crop production. In this study, we examined management tactics to convert perennial legumes to annual crops without tillage. Our hypothesis was that reducing carbohydrate production in the fall by mowing would favor winterkill. Mowing treatments were imposed in the fall of the third year of alfalfa or red clover, and corn planted in year 4. The conventional practice of tillage to convert legumes to cropland was also included as a treatment. Mowing in autumn reduced red clover biomass 93% compared with alfalfa when measured 3 weeks after corn planting (WAP). Red clover biomass was still 75% less than alfalfa 6 WAP. Fall mowing suppressed red clover sufficiently to enable corn seedlings to establish, but corn seedlings did not survive in mowed alfalfa due to alfalfa competition. Corn grain yield following red clover was similar in the mowed and tilled treatments when weeds were present. Late season clover and weed growth reduced corn yields 46% compared with weed-free corn. Weed emergence in corn was three times higher after tillage compared with the mowed treatment. Converting red clover to annual crops with fall mowing will support a no-till system for organic farming. C1 [Anderson, Randy L.] USDA ARS, Brookings, SD 57006 USA. RP Anderson, RL (reprint author), USDA ARS, Brookings, SD 57006 USA. EM randy.anderson@ars.usda.gov NR 20 TC 0 Z9 0 U1 4 U2 9 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD APR PY 2016 VL 31 IS 2 BP 166 EP 171 DI 10.1017/S1742170515000228 PG 6 WC Agriculture, Multidisciplinary SC Agriculture GA DF9WV UT WOS:000371714000007 ER PT J AU Hou, RX Ouyang, Z Maxim, D Wilson, G Kuzyakov, Y AF Hou, Ruixing Ouyang, Zhu Maxim, Dorodnikov Wilson, Glenn Kuzyakov, Yakov TI Lasting effect of soil warming on organic matter decomposition depends on tillage practices SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Thermal adaptation; Warming; Extracellular enzyme activity; No-till; Soil organic matter; Q(10) ID TEMPERATURE SENSITIVITY; MICROBIAL COMMUNITIES; CARBON DECOMPOSITION; ENZYME-ACTIVITY; CLIMATE-CHANGE; FOREST SOILS; NO-TILL; RESPIRATION; RESPONSES; FEEDBACKS AB Global warming accelerates soil organic matter (SOM) decomposition with strong feedback to atmospheric CO2. Such an effect should be especially important for no-till agricultural practices, where SOM accumulates in the topsoil as compared with conventional tillage. We incubated soil samples (0-5 cm) at three temperature levels (15, 21 and 27 degrees C) from long-term till and no-till systems that were in situ warmed and non-warmed to assess the temperature sensitivity of CO2 efflux, labile organic carbon and extracellular enzyme activities. Thermal adaptation to prolonged warming was observed resulting in a lasting effect on SOM decomposition. On average, 26, 14 and 12% more CO2 was emitted at each incubation temperature from the warmed soils compared to the non-warmed soils. The Q(10) value was lower for the warmed than the non-warmed soils. Soil microbial biomass C and dissolved organic C declined with warming. The activities of three extracellular enzymes, beta-glucosidase, chitinase, and sulfatase, were higher under warming and no-till as compared to non-warmed and tilled soil. We concluded that the increased SOM decomposition due to the stimulation of microorganisms by warming was long-lasting. Predictions of C accumulation in the topsoil by no-till farming should be taken with caution, as this C pool is especially vulnerable to global warming. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Hou, Ruixing; Ouyang, Zhu] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China. [Hou, Ruixing; Ouyang, Zhu] Chinese Acad Sci, Yucheng Comprehens Expt Stn, Beijing 100101, Peoples R China. [Maxim, Dorodnikov; Kuzyakov, Yakov] Univ Gottingen, Dept Soil Sci Temperate Ecosyst, D-37077 Gottingen, Germany. [Wilson, Glenn] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Ouyang, Z (reprint author), Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China. EM ouyz@igsnrr.ac.cn RI Kuzyakov, Yakov/D-1605-2010 OI Kuzyakov, Yakov/0000-0002-9863-8461 FU Chinese Academy of Sciences [XDA05050502]; National Natural Science Foundation of China [31300373]; National High-tech Research and Development Program of China [2013AA102903] FX K. Schmidt, I. Ostermeyer and S. Enzmann provided laboratory assistance with sample analysis. This work, including the maintenance of the long-term soil warming experiments, was supported by a grant from the Chinese Academy of Sciences (XDA05050502), and National Natural Science Foundation of China (31300373), and National High-tech Research and Development Program of China (2013AA102903). We express our sincere thanks to the reviewers and editors for their time and effort. NR 35 TC 1 Z9 1 U1 20 U2 69 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD APR PY 2016 VL 95 BP 243 EP 249 DI 10.1016/j.soilbio.2015.12.008 PG 7 WC Soil Science SC Agriculture GA DF7TR UT WOS:000371561000027 ER PT J AU Stenfeldt, C Pacheco, JM Smoliga, GR Bishop, E Pauszek, SJ Hartwig, EJ Rodriguez, LL Arzt, J AF Stenfeldt, C. Pacheco, J. M. Smoliga, G. R. Bishop, E. Pauszek, S. J. Hartwig, E. J. Rodriguez, L. L. Arzt, J. TI Detection of Foot-and-mouth Disease Virus RNA and Capsid Protein in Lymphoid Tissues of Convalescent Pigs Does Not Indicate Existence of a Carrier State SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE foot-and-mouth disease; FMD; FMDV; virus; persistence; carriers; pigs ID FOLLICULAR DENDRITIC CELL; MONOCLONAL-ANTIBODIES; GERMINAL-CENTERS; INFECTED CATTLE; VIRAL PATHWAYS; SECRETORY IGA; SWINE; PATHOGENESIS; SHEEP; TRANSMISSION AB A systematic study was performed to investigate the potential of pigs to establish and maintain persistent foot-and-mouth disease virus (FMDV) infection. Infectious virus could not be recovered from sera, oral, nasal or oropharyngeal fluids obtained after resolution of clinical infection with any of five FMDV strains within serotypes A, O and Asia-1. Furthermore, there was no isolation of live virus from tissue samples harvested at 28-100 days post-infection from convalescent pigs recovered from clinical or subclinical FMD. Despite lack of detection of infectious FMDV, there was a high prevalence of FMDV RNA detection in lymph nodes draining lesion sites harvested at 35 days post-infection, with the most frequent detection recorded in popliteal lymph nodes (positive detection in 88% of samples obtained from non-vaccinated pigs). Likewise, at 35 dpi, FMDV capsid antigen was localized within follicles of draining lymph nodes, but without concurrent detection of FMDV non-structural protein. There was a marked decline in the detection of FMDV RNA and antigen in tissue samples by 60 dpi, and no antigen or viral RNA could be detected in samples obtained at 100 dpi. The data presented herein provide the most extensive investigation of FMDV persistence in pigs. The overall conclusion is that domestic pigs are unlikely to be competent long-term carriers of infectious FMDV; however, transient persistence of FMDV protein and RNA in lymphoid tissues is common following clinical or subclinical infection. C1 [Stenfeldt, C.; Pacheco, J. M.; Smoliga, G. R.; Bishop, E.; Pauszek, S. J.; Hartwig, E. J.; Rodriguez, L. L.; Arzt, J.] USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, POB 848, Greenport, NY 11944 USA. [Stenfeldt, C.] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN USA. RP Arzt, J (reprint author), USDA ARS, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, POB 848, Greenport, NY 11944 USA. EM Jonathan.Arzt@ars.usda.gov OI Pacheco, Juan/0000-0001-5477-0201; Arzt, Jonathan/0000-0002-7517-7893 FU National Pork Board, USA (NPB) [11-174]; Plum Island Animal Disease Center Research Participation Program FX This project was funded through a grant financed by The National Pork Board, USA (NPB project identification number: 11-174). CS is a recipient of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an inter-agency agreement with the US Department of Energy. None of the funding sources had influence upon design or performance of experimental study, interpretation of results or writing of the manuscript. The Korean FMDV isolates were provided by Dr. Kwang-Nyeong Lee, Animal and Plant Quarantine Agency, Minstry of Agriculture, Food and Rural Affairs Republic of Korea. NR 53 TC 5 Z9 5 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD APR PY 2016 VL 63 IS 2 BP 152 EP 164 DI 10.1111/tbed.12235 PG 13 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DF9ZJ UT WOS:000371720900021 PM 24943477 ER PT J AU Ramey, AM Walther, P Link, P Poulson, RL Wilcox, BR Newsome, G Spackman, E Brown, JD Stallknecht, DE AF Ramey, A. M. Walther, P. Link, P. Poulson, R. L. Wilcox, B. R. Newsome, G. Spackman, E. Brown, J. D. Stallknecht, D. E. TI Optimizing Surveillance for South American Origin Influenza A Viruses Along the United States Gulf Coast Through Genomic Characterization of Isolates from Blue-winged Teal (Anas discors) SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE Anas discors; avian influenza; blue-winged teal; influenza A; North America; South America; surveillance; waterfowl ID AVIAN INFLUENZA; HEMAGGLUTININ SUBTYPES; NORTH-AMERICA; WILD BIRDS; RT-PCR; WATERFOWL; ALASKA; DUCKS; ARGENTINA; EXCHANGE AB Relative to research focused on inter-continental viral exchange between Eurasia and North America, less attention has been directed towards understanding the redistribution of influenza A viruses (IAVs) by wild birds between North America and South America. In this study, we genomically characterized 45 viruses isolated from blue-winged teal (Anas discors) along the Texas and Louisiana Gulf Coast during March of 2012 and 2013, coincident with northward migration of this species from Neotropical wintering areas to breeding grounds in the United States and Canada. No evidence of South American lineage genes was detected in IAVs isolated from blue-winged teal supporting restricted viral gene flow between the United States and southern South America. However, it is plausible that blue-winged teal redistribute IAVs between North American breeding grounds and wintering areas throughout the Neotropics, including northern South America, and that viral gene flow is limited by geographical barriers further south (e.g. the Amazon Basin). Surveillance for the introduction of IAVs from Central America and northern South America into the United States may be further optimized through genomic characterization of viruses resulting from coordinated, concurrent sampling efforts targeting blue-winged teal and sympatric species throughout the Neotropics and along the United States Gulf Coast. C1 [Ramey, A. M.] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. [Ramey, A. M.; Poulson, R. L.; Wilcox, B. R.; Brown, J. D.; Stallknecht, D. E.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Walther, P.] US Fish & Wildlife Serv, Texas Chenier Plain Refuge Complex, Anahuac, TX USA. [Link, P.] Louisiana Dept Wildlife & Fisheries, Baton Rouge, LA USA. [Newsome, G.] City Beaumont Wastewater Treatment Plant, Beaumont, TX USA. [Spackman, E.] USDA ARS, Southeast Poultry Res Lab, Athens, GA 30613 USA. RP Ramey, AM (reprint author), US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. EM aramey@usgs.gov FU U.S. Geological Survey through the Wildlife Program of the Ecosystem Mission Area; National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH); Department of Health and Human Services [HHSN 266200700007C] FX We are grateful to many biologists and volunteers for their assistance with captures including P. Pauling, P. Oesterle, W. Broussard, A. Fojtik, D. Carter, J. Slagter, N. Davis-Fields, J. Starr, J. LaCour, J. Gray and K. DeMarco. We thank the City of Beaumont, Texas, for access to Cattail Marsh for captures conducted in 2012 and 2013. We appreciate assistance provided by J. Terenzi with the creation of Fig. 2. Reviews provided by K. McCracken, J. Pearce, C. Lebarbenchon and two anonymous reviewers were used to improve the quality of this manuscript. Administrative support was provided by J. Pearce, T. DeGange, P. Bright, K. Briggs and S. Gross. This work was funded by the U.S. Geological Survey through the Wildlife Program of the Ecosystem Mission Area and by the National Institute of Allergy and Infectious Diseases, National Institutes of Health (NIH) and the Department of Health and Human Services under contract HHSN 266200700007C. The opinions expressed herein do not necessarily reflect the views of the latter two funding agencies. None of the authors have any financial interests or conflict of interest with this article. Any use of trade names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 39 TC 2 Z9 2 U1 0 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD APR PY 2016 VL 63 IS 2 BP 194 EP 202 DI 10.1111/tbed.12244 PG 9 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DF9ZJ UT WOS:000371720900025 PM 25056712 ER PT J AU Simsek, H Kasi, M Ohm, JB Murthy, S Khan, E AF Simsek, Halis Kasi, Murthy Ohm, Jae-Bom Murthy, Sudhir Khan, Eakalak TI Impact of solids retention time on dissolved organic nitrogen and its biodegradability in treated wastewater SO WATER RESEARCH LA English DT Article DE Biodegradable dissolved organic nitrogen; Non-biodegradable dissolved organic nitrogen; Dissolved organic nitrogen; Solids retention time; Wastewater ID SOLUBLE MICROBIAL PRODUCTS; ACTIVATED-SLUDGE; TREATMENT SYSTEMS; TREATMENT PLANTS; EFFLUENTS; SMP; SPECIATION; CARBON; FATE AB Dissolved organic nitrogen (DON) and its biodegradability in treated wastewater have recently gained attention due to increased regulatory requirements on effluent quality to protect receiving waters. Laboratory scale chemostat experiments were conducted at 9 different solids retention times (SRTs) (0.3, 0.7, 2, 3, 4, 5, 7, 8, and 13 days) to examine whether SRT could be used to control DON, biodegradable DON (BDON), and DON biodegradability (BDON/DON) levels in treated wastewater. Results indicated no trend between effluent DON and SRTs. Effluent BDON was comparable for SRTs of 0.3-4 days and had a decreasing trend with SRT after that. Effluent DON biodegradability (effluent BDON/effluent DON) ranging from 23% to 59% tended to decrease with SRT. Chemostat during longer SRT5, however, was contributing to non-biodegradable DON (NBDON) and this fraction of DON increased with SRT above 4 days. Model calibration results indicated that ammonification rate, and growth rates for ordinary heterotrophs, ammonia oxidizing bacteria and nitrite oxidizing bacteria were not constants but have a decreasing trend with increasing SRT. This study indicates the benefit of high SRTs in term of producing effluent with less DON biodegradability leading to relatively less oxygen consumption and nutrient support in receiving waters. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Simsek, Halis] N Dakota State Univ, Dept Agr & Biosyst Engn, Fargo, ND 58108 USA. [Kasi, Murthy] HDR Inc, Boise, ID 83706 USA. [Ohm, Jae-Bom] N Dakota State Univ, USDA ARS, Hard Red Spring & Durum Wheat Qual Lab, Harris Hall, Fargo, ND 58108 USA. [Murthy, Sudhir] Dist Columbia Water & Sewer Author, Washington, DC 20032 USA. [Khan, Eakalak] N Dakota State Univ, Dept Civil & Environm Engn, Dept 2470,POB 6050, Fargo, ND 58108 USA. RP Khan, E (reprint author), N Dakota State Univ, Dept Civil & Environm Engn, Dept 2470,POB 6050, Fargo, ND 58108 USA. EM eakalak.khan@ndsu.edu OI Kasi, Murthy/0000-0003-0343-1140; Khan, Eakalak/0000-0002-6729-2170 FU District of Columbia Water and Sewer Authority (DC Water), Washington, DC, USA FX Funding for this research was provided by the District of Columbia Water and Sewer Authority (DC Water), Washington, DC, USA. The authors would like to thank Andrew Bradshaw, Darsey Thoen, Maria Amundson, and Kyle Juntila, the Moorhead WWTF, Moorhead, MN, USA for their guidance and friendly help with wastewater sample collection. NR 36 TC 3 Z9 3 U1 9 U2 36 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD APR 1 PY 2016 VL 92 BP 44 EP 51 DI 10.1016/j.watres.2016.01.041 PG 8 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DF7RL UT WOS:000371555200005 PM 26841227 ER PT J AU Silva, FML Duke, SO Dayan, FE Velini, ED AF Silva, F. M. L. Duke, S. O. Dayan, F. E. Velini, E. D. TI Low doses of glyphosate change the responses of soyabean to subsequent glyphosate treatments SO WEED RESEARCH LA English DT Article DE hormesis; glyphosate; soyabean; reduced dose; dose-response ID GLYCINE-MAX; HERBICIDE; RESISTANT; HORMESIS; PLANTS; STRESS; GROWTH; METABOLISM; DRIFT AB Many herbicides promote plant growth at doses well below the recommended application rate (hormesis). The objectives of this study were to evaluate glyphosate-induced hormesis in soyabean (Glycine max) and determine whether pre-treating soyabean seedlings with low doses of glyphosate would affect their response to subsequent glyphosate treatments. Seven doses (1.8-720g a.e. ha(-1)) of glyphosate were applied to 3-week-old seedlings, and the effects on the electron transport rate (ETR), metabolite (shikimate, benzoate, salicylate, AMPA, phenylalanine, tyrosine and tryptophan) levels and dry weight were determined. The lowest dose stimulated ETR and increased biomass the most. Benzoate levels increased 203% with 3.6g a.e. ha(-1) glyphosate. Salicylate content and tyrosine content were unaffected, whereas phenylalanine and tryptophan levels were increased by 60 and 80%, respectively, at 7.2g a.e. ha(-1). Dose-response curves for these three amino acids were typical for hormesis. In another experiment that was replicated twice, soyabean plants were pre-treated with low doses of glyphosate (1.8, 3.6 or 7.2g a.e. ha(-1)) and treated with a second application of glyphosate (1.8, 3.6, 7.2, 36, 180 or 720g a.e. ha(-1)) 14days later. For total seedling dry weight, a 3.6 and 7.2g a.e. ha(-1) glyphosate dose preconditioned the soyabean seedlings to have greater growth stimulation by a later glyphosate treatment than plants with no preconditioning glyphosate exposure. Optimal hormetic doses were generally higher with pre-treated plants than plants that had not been exposed to glyphosate. Thus, pre-exposure to low doses of glyphosate can change the hormetic response to later low-dose exposures. C1 [Silva, F. M. L.; Velini, E. D.] Sao Paulo State Univ, Fac Agron Sci, Botucatu, SP, Brazil. [Duke, S. O.; Dayan, F. E.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS USA. RP Duke, SO (reprint author), ARS, Nat Prod Utilizat Res Unit, USDA, Cochran Ctr, POB 1848, University, MS 38677 USA. EM Stephen.Duke@ars.usda.gov OI Dayan, Franck/0000-0001-6964-2499 FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) FX We thank Robert Johnson for his technical help in the glasshouse. Thanks to Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for financial support to the first author. NR 36 TC 1 Z9 1 U1 5 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD APR PY 2016 VL 56 IS 2 BP 124 EP 136 DI 10.1111/wre.12189 PG 13 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DG2PF UT WOS:000371909000004 ER PT J AU Flores, D AF Flores, David TI Fighting for Peace: Veterans and Military Families in the Anti-Iraq War Movement SO ARMED FORCES & SOCIETY LA English DT Book Review C1 [Flores, David] US Forest Serv, Albuquerque, NM USA. RP Flores, D (reprint author), US Forest Serv, Albuquerque, NM USA. NR 2 TC 0 Z9 0 U1 1 U2 1 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0095-327X EI 1556-0848 J9 ARMED FORCES SOC JI Armed Forces Soc. PD APR PY 2016 VL 42 IS 2 BP 472 EP 474 DI 10.1177/0095327X15574257 PG 3 WC Political Science; Sociology SC Government & Law; Sociology GA DF4GZ UT WOS:000371307300014 ER PT J AU Greenfield, M Jimenez, MIG Ortiz, V Vega, FE Kramer, M Parsa, S AF Greenfield, Melinda Gomez Jimenez, Maria I. Ortiz, Viviana Vega, Fernando E. Kramer, Matthew Parsa, Soroush TI Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation SO BIOLOGICAL CONTROL LA English DT Article DE Beauveria; Cassava; Endophyte; Fungal entomopathogen; Metarhizium ID BERGI FROESCHNER HEMIPTERA; ASCOMYCOTA-HYPOCREALES; FUNGAL ENDOPHYTES; ENTOMOPATHOGENIC FUNGI; PAPAVER-SOMNIFERUM; BALSAMO VUILLEMIN; METSCH. SOROKIN; PEST-MANAGEMENT; SEED TREATMENT; PLANTS AB We investigated the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization of cassava roots by both entomopathogens, though neither was found in the leaves or stems of the treated cassava plants. Both fungal entomopathogens were detected more often in the proximal end of the root than in the distal end. Colonization levels of B. bassiana were higher when plants were sampled at 7-9 days post-inoculation (84%) compared to 47-49 days post-inoculation (40%). In contrast, the colonization levels of M. anisopliae remained constant from 7-9 days post -inoculation (80%) to 47-49 days post inoculation (80%), which suggests M. anisopliae is better able to persist in the soil, or as an endophyte in cassava roots over time. Differences in colonization success and plant growth were found among the fungal entomopathogen treatments. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). C1 [Greenfield, Melinda; Gomez Jimenez, Maria I.; Ortiz, Viviana; Parsa, Soroush] Int Ctr Trop Agr CIAT, Entomol, Km 17, Recta Cali Palmira, Colombia. [Vega, Fernando E.] USDA ARS, Sustainable Perennial Crops Lab, Bldg 001, Beltsville, MD 20705 USA. [Kramer, Matthew] USDA ARS, Stat Grp, Beltsville, MD 20705 USA. [Parsa, Soroush] Univ Calif Davis, Life Sci Innovat Ctr, Andres Bello 2299 1102, Santiago, Chile. RP Greenfield, M (reprint author), James Cook Univ, Coll Marine & Environm Sci, Bldg E1,POB 6811, Cairns, Qld 4870, Australia. EM melinda.greenfield@my.jcu.edu.au; m.i.gomez@cgiar.org; vivianaortlond@gmail.com; Fernando.Vega@ars.usda.gov; Matt.Kramer@ars.usda.gov; sparsa@ucdavischile.org OI Vega, Fernando E./0000-0001-8103-5640 FU Bill and Melinda Gates Foundation Grand Challenges Exploration Grant [OPP1069291] FX This project was supported by a Bill and Melinda Gates Foundation Grand Challenges Exploration Grant (#OPP1069291, Endophytic biological control for cassava and beans) to S. Parsa and F. E. Vega. We thank Reynaldo Pareja and Leidy Sanchez Rey for technical support in the laboratory and greenhouse, and Adriano Munoz, Gerardino Perez, Harrison Moran, Jose F. Valor, and Rodrigo Zuniga for their assistance in the greenhouse. We would also like to thank Mark Jackson (USDA, ARS, Peoria, IL) for advice during our greenhouse experiments. NR 65 TC 3 Z9 4 U1 12 U2 42 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2016 VL 95 BP 40 EP 48 DI 10.1016/j.biocontrol.2016.01.002 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DF6CZ UT WOS:000371444700006 ER PT J AU Cheng, Z Chi, MS Li, GK Chen, HZ Sui, Y Sun, H Wisniewski, M Liu, YS Liu, J AF Cheng, Zhe Chi, Mengshan Li, Guangkun Chen, Huizhen Sui, Yuan Sun, Hanjv Wisniewski, Michael Liu, Yongsheng Liu, Jia TI Heat shock improves stress tolerance and biocontrol performance of Rhodotorula mucilaginosa SO BIOLOGICAL CONTROL LA English DT Article DE Antioxidant system; Biocontrol yeast; Cross-protection; Decay control; Heat shock; Stress tolerance ID SACCHAROMYCES-CEREVISIAE KNU5377; YEAST CANDIDA-SAKE; OXIDATIVE STRESS; POSTHARVEST DISEASES; EFFICACY; ANTIOXIDANT; TEMPERATURE; GENE; INDUCTION; INCREASE AB Biological control of postharvest diseases, utilizing wild species and strains of antagonistic yeasts, is an eco-friendly management approach that has received considerable attention over the past three decades. A few yeast-based biocontrol products are either in advanced stages of development or commercially available. Ecological fitness, stress tolerance and biocontrol performance are essential attributes for yeasts used as biocontrol agents. In the present study, a mild heat shock (HS, 40 degrees C for 30 min) pretreatment of the biocontrol yeast, Rhodotorula mucilaginosa, increased its tolerance to subsequent high temperature (48 degrees C), oxidative stress (70 mM H2O2), salt stress (3 M NaCl), and low pH (pH 4). Activation of the yeast antioxidant system by HS resulted in a lower accumulation of reactive oxygen species and less oxidative damage to proteins and lipids relative to control yeast cells when both were subjected to various abiotic stresses. Additionally, HS-treated yeast exhibited increased efficacy in controlling infection of apple fruit by Penicillium expansum, and a higher growth rate in apple wounds. These results suggest that stress adaptation is a viable approach for improving the efficacy of yeast biocontrol agents under the variable environmental conditions that exist in the postharvest environment of packing houses and storage facilities. (C) 2016 Elsevier Inc. All rights reserved. C1 [Cheng, Zhe; Chi, Mengshan; Li, Guangkun; Chen, Huizhen; Sui, Yuan; Sun, Hanjv; Liu, Yongsheng; Liu, Jia] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. [Wisniewski, Michael] USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Liu, J (reprint author), Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. EM jialiu1983@163.com FU National Natural Science Foundation of China [31501688, 31401794, 31461143008]; China Postdoctoral Science Foundation [2015M570534]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry; Provincial Natural Science Research Project of Anhui Colleges [KJ2014ZD25]; Qiushi Project of HFUT [JZ2015QSJH0217] FX This work was supported by grants from the National Natural Science Foundation of China (Nos. 31501688, 31401794 & 31461143008), a China Postdoctoral Science Foundation Funded Project (No. 2015M570534), the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry to Yuan Sui, the Provincial Natural Science Research Project of Anhui Colleges (No. KJ2014ZD25) and Qiushi Project of HFUT (No. JZ2015QSJH0217). NR 35 TC 0 Z9 0 U1 7 U2 32 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2016 VL 95 BP 49 EP 56 DI 10.1016/j.biocontrol.2016.01.001 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DF6CZ UT WOS:000371444700007 ER PT J AU Meagher, RL Nuessly, GS Nagoshi, RN Hay-Roe, MM AF Meagher, Robert L., Jr. Nuessly, Gregg S. Nagoshi, Rodney N. Hay-Roe, Mirian M. TI Parasitoids attacking fall armyworm (Lepidoptera: Noctuidae) in sweet corn habitats SO BIOLOGICAL CONTROL LA English DT Article DE Spodoptera frugiperda; Cotesia marginiventris; Chelonus insularis; Braconidae ID SPODOPTERA-FRUGIPERDA LEPIDOPTERA; COTESIA-MARGINIVENTRIS HYMENOPTERA; HIPPODAMIA-CONVERGENS COLEOPTERA; EIPHOSOMA-VITTICOLE HYMENOPTERA; GEOCORIS-PUNCTIPES HEMIPTERA; CHELONUS-INSULARIS CRESSON; NATURAL ENEMIES; POPULATION-DYNAMICS; BIOLOGICAL-CONTROL; INTERSPECIFIC COMPETITION AB Fall armyworm larvae, Spodoptera frugiperda (J. E. Smith), were collected from sweet corn plants (Zea mays L.) in fields located in three south Florida counties. Fields were sampled from 2010 to 2015 during the fall and spring seasons. Larvae were transferred to the laboratory to complete development. The objective of the study was to identify the common parasitoids emerging from larvae that are present in sweet corn habitats where insecticides are traditionally used. A total of 8353 fall armyworm larvae were collected, of which 60.6% (5062) developed into moths after feeding on corn tissue and artificial diet. Parasitoids emerged from 2365 larvae (28.3%), and parasitism ranged from 1% to 91.7%, depending on site. Parasitism was higher at the University of Florida Everglades Research and Education (EREC) in Belle Glade (50.4 +/- 11.8%) than at other locations in south Florida. Parasitism was comparable between fall and spring seasons, but was much higher in unsprayed fields (44.0 +/- 9.6%) than in the sprayed fields (15.0 +/- 2.5%). The two most common parasitoids that emerged from larvae were the solitary endoparasitoids Cotesia marginiventris (Cresson), found in 23 of the 25 sites sampled, and Chelonus insularis Cresson, found in 18 of the 25 sites sampled. Other parasitoid species that emerged from fall armyworm larvae were Aleiodes laphygmae (Viereck), Euplectrus platyhypenae Howard, Meteorus spp., Ophion flavidus Brulle, and unidentified species of Tachinidae. Techniques to improve the management of fall armyworm in overwintering areas of south Florida using conservation biological control are discussed. Published by Elsevier Inc. C1 [Meagher, Robert L., Jr.; Nagoshi, Rodney N.] USDA ARS, Ctr Med Agr & Vet Entomol, Insect Behav & Biocontrol Res Unit, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. [Nuessly, Gregg S.; Hay-Roe, Mirian M.] Univ Florida, Inst Food & Agr Sci, Everglades Res & Educ Ctr, 3200 E Palm Beach Rd, Belle Glade, FL 33430 USA. RP Meagher, RL (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Insect Behav & Biocontrol Res Unit, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM rob.meagher@ars.usda.gov; gnuessly@ufl.edu; Rodney.nagoshi@ars.usda.gov; mirian.hay-roe@ars.usda.gov FU Monsanto Co. FX We thank the sweet corn growers in south Florida who made their fields available for our collections. The assistance of A. Rowley, J. Brown, S. Velez, N. Fieleke (USDA-ARS), N. Larsen, D. Owens (UF-EREC), and T. Spencer (Univ. of Nebraska) is greatly appreciated. This research was supported by Monsanto Co. NR 77 TC 0 Z9 0 U1 5 U2 19 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2016 VL 95 BP 66 EP 72 DI 10.1016/j.biocontrol.2016.01.006 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DF6CZ UT WOS:000371444700009 ER PT J AU Goble, TA Gardescu, S Fisher, JJ Jackson, MA Hajek, AE AF Goble, Tarryn A. Gardescu, Sana Fisher, Joanna J. Jackson, Mark A. Hajek, Ann E. TI Conidial production, persistence and pathogenicity of hydromulch formulations of Metarhizium brunneum F52 microsclerotia under forest conditions SO BIOLOGICAL CONTROL LA English DT Article DE Entomopathogenic fungus; Environmental persistence; Anoplophora glabripennis; Augmentation biological control ID ASIAN LONGHORNED BEETLE; ANOPLOPHORA-GLABRIPENNIS MOTSCHULSKY; BIOLOGICAL-CONTROL AGENTS; COLEOPTERA CERAMBYCIDAE; FUNGAL ENTOMOPATHOGENS; BEAUVERIA-BRONGNIARTII; FIBER BANDS; ANISOPLIAE; EFFICACY; TEMPERATURE AB Microsclerotia granules of Metarhizium brunneum Petch strain F52 (Hypocreales: Clavicipitaceae) in a hydromulch formulation (water, wheat straw, and tackifier) were sprayed onto bark or wood samples, which were subsequently exposed on tree trunks for 4-30 d, during two spray trials in 2013 and six spray trials in 2014. Microsclerotial granules in this formulation continued to produce many infective conidia over a 30 d period in a forest. Greater conidial production was associated with periods of increased temperature, relative humidity and rainfall. When F52-hydromulch-treated samples, in the 2014 spray trials, were evaluated against adult Asian longhorned beetles, Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), in 2 d-quarantine bioassays, insect mortality varied greatly for males (14-92%) and females (56-100%). Highest conidial densities (5.5 x 10(6) conidia/cm(2)) were reached after 2030 d in the field and at these densities, female beetle median survival times ranged from 15.5 to 21.5 d. Males died significantly slower in all spray trials. The continued increase in conidial production by microsclerotia in hydromulch over time suggests that reapplication of a potential product in the field might only need to occur after 6 weeks. (C) 2016 Elsevier Inc. All rights reserved. C1 [Goble, Tarryn A.; Gardescu, Sana; Fisher, Joanna J.; Hajek, Ann E.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Jackson, Mark A.] USDA ARS, NCAUR, Crop Bioprotect Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. RP Goble, TA (reprint author), Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. EM tazgoble@gmail.com OI Fisher, Joanna/0000-0002-7060-9749 FU Litwin Foundation FX We thank our laboratory assistants and technicians, especially Jake Henry, Lauren Smith, Connor Roberson, Sylvan Whitmore and Grace Mitchell. A very special thank you goes to Jeff Gardner for assisting in the development of the spray applications, and Francoise Vermeylen and Todd Ugine for statistical consultations. Thanks to Ed Lee for providing hydromulch and technical support, Steve Vaughn for hydromulch grinding, Cole Gilbert for providing maple bark, and the reviewer for helpful comments. Finally, we thank the Alphawood Foundation for their support of the beetle colony and the Litwin Foundation for research support. NR 45 TC 1 Z9 1 U1 6 U2 18 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2016 VL 95 BP 83 EP 93 DI 10.1016/j.biocontrol.2016.01.003 PG 11 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DF6CZ UT WOS:000371444700011 ER PT J AU DeDonder, KD Apley, MD Li, M Gehring, R Harhay, DM Lubbers, BV White, BJ Capik, SF Kukanich, B Riviere, JE Tessman, RK AF DeDonder, K. D. Apley, M. D. Li, M. Gehring, R. Harhay, D. M. Lubbers, B. V. White, B. J. Capik, S. F. Kukanich, B. Riviere, J. E. Tessman, R. K. TI Pharmacokinetics and pharmacodynamics of gamithromycin in pulmonary epithelial lining fluid in naturally occurring bovine respiratory disease in multisource commingled feedlot cattle SO JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS LA English DT Article ID LUNG-TISSUE CONCENTRATIONS; BRONCHOALVEOLAR CELLS; LEUKOTRIENE B-4; TULATHROMYCIN; ANTIBIOTICS; APOPTOSIS; PLASMA; EFFEROCYTOSIS; DISPOSITION; TILMICOSIN AB The objectives of this study were to determine (i) whether an association exists between individual pharmacokinetic parameters and treatment outcome when feeder cattle were diagnosed with bovine respiratory disease (BRD) and treated with gamithromycin (Zactran((R))) at the label dose and (ii) whether there was a stronger association between treatment outcome and gamithromycin concentration in plasma or in the pulmonary epithelial lining fluid (PELF) effect compartment. The study design was a prospective, blinded, randomized clinical trial utilizing three groups of 60 (362-592lb) steers/bulls randomly allocated within origin to sham injection or gamithromycin mass medication. Cattle were evaluated daily for signs of BRD by a veterinarian blinded to treatment. Animals meeting the BRD case definition were enrolled and allocated to a sample collection scheme consisting of samples for bacterial isolation (bronchoalveolar lavage fluid and nasopharyngeal swabs) and gamithromycin concentration determination (PELF and plasma). Gamithromycin susceptibility of M.haemolytica (n=287) and P.multocida (n=257) were determined using broth microdilution with frozen panels containing gamithromycin at concentrations from 0.03 to 16g/mL. A two-compartment plasma pharmacokinetic model with an additional compartment for gamithromycin in PELF was developed using rich data sets from published and unpublished studies. The sparse data from our study were then fit to this model using nonlinear mixed effects modeling to estimate individual parameter values. The resulting parameter estimates were used to simulate full time-concentration profiles for each animal in this study. These profiles were analyzed using noncompartmental methods so that PK/PD indices (AUC(24)/MIC, AUC/MIC, C-MAX/MIC) could be calculated for plasma and PELF (also T>MIC) for each individual. The calculated PK/PD indices were indicative that for both M.haemolytica and P.multocida a higher drug exposure in terms of concentration, and duration of exposure relative to the MIC of the target pathogen, was favorable to a successful case outcome. Asignificant association was found between treatment success and PELF AUC(0-24)/MIC for P.multocida. The calves in this study demonstrated an increased clearance and volume of distribution in plasma as compared to the healthy calves in two previously published reports. Ultimately, the findings from this study indicate that higher PK/PD indices were predictive of positive treatment outcomes. C1 [DeDonder, K. D.; Capik, S. F.] Kansas State Univ, Coll Vet Med, Diagnost Med Pathobiol, 1710 Denison Ave P200 Mosier Hall, Manhattan, KS 66506 USA. [Apley, M. D.; White, B. J.] Kansas State Univ, Coll Vet Med, Clin Sci, Manhattan, KS 66506 USA. [Li, M.; Gehring, R.; Riviere, J. E.] Kansas State Univ, Coll Vet Med, Inst Computat Comparat Med, Manhattan, KS 66506 USA. [Harhay, D. M.] USDA, ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Lubbers, B. V.] Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, Manhattan, KS 66506 USA. [Kukanich, B.] Kansas State Univ, Coll Vet Med, Anat & Physiol, Manhattan, KS 66506 USA. [Tessman, R. K.] Pharmaceut Res & Dev, Duluth, GA USA. RP DeDonder, KD (reprint author), Kansas State Univ, Coll Vet Med, Diagnost Med Pathobiol, 1710 Denison Ave P200 Mosier Hall, Manhattan, KS 66506 USA. EM keith@mvsinc.net FU Merial; Duluth; Georgia FX We wish to acknowledge that this study was financially supported, in part, by Merial, Duluth, and Georgia. NR 29 TC 1 Z9 1 U1 7 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7783 EI 1365-2885 J9 J VET PHARMACOL THER JI J. Vet. Pharmacol. Ther. PD APR PY 2016 VL 39 IS 2 BP 157 EP 166 DI 10.1111/jvp.12267 PG 10 WC Pharmacology & Pharmacy; Veterinary Sciences SC Pharmacology & Pharmacy; Veterinary Sciences GA DG0DR UT WOS:000371733600007 PM 26441021 ER PT J AU Kaucher, A Park, KE Oatley, M Powell, A Donovan, DM Blomberg, LA Lillico, S Whitelaw, B Mileham, A Telugu, B Oatley, J AF Kaucher, Amy Park, Ki-Eun Oatley, Melissa Powell, Anne Donovan, David M. Blomberg, Le Ann Lillico, Simon Whitelaw, Bruce Mileham, Alan Telugu, Bhanu Oatley, Jon TI Production of germline ablated male pigs via Crispr/Cas editing of the NANOS2 gene SO TRANSGENIC RESEARCH LA English DT Meeting Abstract DE CRISPR/Cas; gene editing; livestock; germline; fertility C1 [Kaucher, Amy; Oatley, Melissa; Oatley, Jon] Washington State Univ, Coll Vet Med, Ctr Reprod Biol, Pullman, WA 99164 USA. [Park, Ki-Eun; Telugu, Bhanu] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Park, Ki-Eun; Powell, Anne; Donovan, David M.; Blomberg, Le Ann; Telugu, Bhanu] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD USA. [Lillico, Simon; Whitelaw, Bruce] Univ Edinburgh, Roslin Inst, Edinburgh, Midlothian, Scotland. [Mileham, Alan] Genus PIC, De Forest, WI USA. NR 0 TC 0 Z9 0 U1 5 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD APR PY 2016 VL 25 IS 2 BP 209 EP 210 PG 2 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DF2FD UT WOS:000371155900028 ER PT J AU Die, JV Arora, R Rowland, LJ AF Die, Jose V. Arora, Rajeev Rowland, Lisa J. TI Global patterns of protein abundance during the development of cold hardiness in blueberry SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Blueberry; Cold acclimation; Low temperature; Mass spectrometry; Proteomics; 2D-DIGE ID LOW-TEMPERATURE; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; FREEZING TOLERANCE; STRESS TOLERANCE; FLORAL BUDS; POLYPHENOL OXIDASE; DROUGHT STRESS; ABIOTIC STRESS; TRANSCRIPTIONAL ACTIVATOR AB To gain a better understanding of the cold acclimation progression in blueberry, we investigated the proteome-level changes that occur in flower buds with increasing exposure to chilling temperatures using the 2D-DIGE technique. From this procedure, 104 protein spots were found to be differentially expressed. These proteins, identified by mass spectrometry, were compared to those previously found on 1-D protein gels and to differentially expressed transcripts from an earlier transcriptome study. The most highly induced proteins corresponded to previously described dehydrins. Approximately half of the changes in the proteome reflected similar changes in the transcriptome. In addition, from 2D-DIGE, different quantitative patterns of protein induction and suppression were found. The largest differences occurred during the transition from the first to the second stage of cold acclimation, which corresponded to timing of the largest increase in cold hardiness. This, with qualitative differences affecting the regulation of several functional groups, suggest as a whole that plants are able to monitor changes in the environment and then respond by modulating their proteome accordingly. Major pathways increasing in abundance included stress-related proteins, carbohydrate/energy metabolism, amino acid metabolism, biosynthesis of phenolic compounds and gene expression regulation. On the other hand, pathways decreasing in abundance consisted of stress-related proteins, photosynthetic proteins and cell growth and structural components. Their possible implication in the development of cold hardiness is discussed. Published by Elsevier B.V. C1 [Die, Jose V.; Rowland, Lisa J.] ARS, USDA, Beltsville, MD USA. [Arora, Rajeev] Iowa State Univ, Dept Hort, Ames, IA USA. RP Rowland, LJ (reprint author), ARS, USDA, Beltsville, MD USA. EM Jeannine.Rowland@ars.usda.gov OI Die, Jose V./0000-0002-7506-8590 NR 79 TC 0 Z9 0 U1 3 U2 32 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 EI 1873-7307 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD APR PY 2016 VL 124 BP 11 EP 21 DI 10.1016/j.envexpbot.2015.12.003 PG 11 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA DE8SP UT WOS:000370907300002 ER PT J AU Saud, P Lynch, TB Anup, KC Guldin, JM AF Saud, Pradip Lynch, Thomas B. Anup, K. C. Guldin, James M. TI Using quadratic mean diameter and relative spacing index to enhance height-diameter and crown ratio models fitted to longitudinal data SO FORESTRY LA English DT Article DE mixed-effects model; autocorrelation; height-d.b.h. relationship; crown ratio; quadratic mean diameter; relative spacing index ID AREA INCREMENT MODEL; SCOTS PINE; VOLUME EQUATIONS; INDIVIDUAL TREES; TAPER EQUATIONS; FORESTS; STANDS; GROWTH; CHINA; STRATEGIES AB The inclusion of quadratic mean diameter (QMD) and relative spacing index (RSI) substantially improved the predictive capacity of height-diameter at breast height (d.b.h.) and crown ratio models (CR), respectively. Data were obtained from 208 permanent plots established in western Arkansas and eastern Oklahoma during 1985-1987 and remeasured for the sixth time (2012-2014). Existing height-d.b.h. and CR estimation models for naturally occurring shortleaf pine forests (Pinus echinata Mill.) were updated and modified for improved performance. Additionally, eight height-d.b.h. relationship models that use only d.b.h. (fundamental local models) were modified using covariates. The model performance was evaluated using fit statistics [root mean square error (RMSE), Fit index and Akaike information criteria (AIC)]. The results showed that the best model form which was an extended non-linear model with autoregressive first order AR(1) structure and power variance function performed better than extended mixed-effects models and predicted well as an ordinary least squares non-linear model. The autocorrelation within individual trees was larger for the height-d.b.h. relationship than for CR estimation. The addition of QMD to mean dominant height (HD) greatly improved height-d.b.h. relationship with a reduction of 8 per cent in RMSE, compared with the use of basal area per hectare. Similarly, multiplying a fundamental local model by using QMD raised to a parametric power reduced RMSE by 16 per cent, improved Fit index by 12 per cent and decreased the AIC value by 7 per cent. D.b.h., HD and RSI best explained the crown ratio relationship with an improved Fit index by 6.7 per cent compared with alternative non-linear models without RSI. The logistic model for CR also provided prediction accuracy similar to that of a commonly used non-linear model. Anon-linear model with an application of remedial measures to enhance adherence to modelling assumptions can provide better parameter estimates than mixed-effects modelling approach. C1 [Saud, Pradip; Lynch, Thomas B.; Anup, K. C.] Oklahoma State Univ, Dept Nat Resource Ecol & Management, Ag Hall Room 008C, Stillwater, OK 74078 USA. [Guldin, James M.] US Forest Serv, Southern Res Stn, Hot Spring, AR 71902 USA. RP Saud, P (reprint author), Oklahoma State Univ, Dept Nat Resource Ecol & Management, Ag Hall Room 008C, Stillwater, OK 74078 USA. EM pradipsaud@hotmail.com OI Saud, Pradip/0000-0002-2045-2827 FU USDA Forest Service [11-C11330124-042] FX Grant number 11-C11330124-042, 25 Year Remeasurement of Natural Evenaged Shortleaf Pine Growth and Yield Study, Source: USDA Forest Service. NR 57 TC 0 Z9 0 U1 6 U2 12 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD APR PY 2016 VL 89 IS 2 BP 215 EP 229 DI 10.1093/forestry/cpw004 PG 15 WC Forestry SC Forestry GA DE9PK UT WOS:000370970000012 ER PT J AU Fynn, RWS Augustine, DJ Peel, MJS de Garine-Wichatitsky, M AF Fynn, Richard W. S. Augustine, David J. Peel, Michael J. S. de Garine-Wichatitsky, Michel TI Strategic management of livestock to improve biodiversity conservation in African savannahs: a conceptual basis for wildlife-livestock coexistence SO JOURNAL OF APPLIED ECOLOGY LA English DT Review DE African savanna; disease management; ecosystem engineering; ecosystem services; functional heterogeneity; herbivore coexistence; pastoralism; protected areas; resource partitioning; wildlife-livestock interactions ID LARGE HERBIVORES; POPULATION DECLINES; DOMESTIC HERBIVORES; GRAZING RUMINANTS; SEMIARID SAVANNA; PROTECTED AREAS; LAND-USE; CATTLE; DYNAMICS; HETEROGENEITY AB African savannas are complex socio-ecological systems with diverse wild and domestic herbivore assemblages, which adapt spatially to intra- and interannual variation in forage quantity and quality, predation and disease risks. As African savannas become increasingly fragmented by growing human populations and their associated ecological impacts, adaptive foraging options for wild and domestic herbivore populations are correspondingly limited, resulting in declining wildlife populations and impoverished pastoral societies. In addition, competition for grazing by expanding domestic herbivore populations threatens the viability of wild herbivore populations occupying similar grazing niches. Conservation initiatives are further impacted by conflicts between wildlife and local communities of people who often receive little benefit from adjacent protected areas, creating conflict between the livelihood-orientated goals of communities and the conservation-oriented goals of the international community and those with vested interests in wildlife. Conservation strategies facilitating the alignment of these opposing goals of communities and conservationists are needed.Synthesis and applications. Key to understanding facilitative and competitive interactions between wild and domestic herbivores are the concepts of niche differentiation and functional resource heterogeneity. Uncontrolled incursions of burgeoning domestic herbivore populations into protected areas (PAs) threaten the conservation of wild herbivore biodiversity. However, domestic herbivores can be managed to minimize competition with wild herbivores and to enhance habitat by maximizing grassland structural heterogeneity (greater adaptive foraging options), creation of nutrient hotspots in the landscape and facilitation of high-quality grazing. Ecosystem service benefits to communities through controlled access to grazing resources in PAs, associated with appropriate disease management, can provide a conservation payment to promote communities' support of conservation of key wildlife migratory ranges and corridors outside PAs. Key to understanding facilitative and competitive interactions between wild and domestic herbivores are the concepts of niche differentiation and functional resource heterogeneity. Uncontrolled incursions of burgeoning domestic herbivore populations into protected areas (PAs) threaten the conservation of wild herbivore biodiversity. However, domestic herbivores can be managed to minimize competition with wild herbivores and to enhance habitat by maximizing grassland structural heterogeneity (greater adaptive foraging options), creation of nutrient hotspots in the landscape and facilitation of high-quality grazing. Ecosystem service benefits to communities through controlled access to grazing resources in PAs, associated with appropriate disease management, can provide a conservation payment to promote communities' support of conservation of key wildlife migratory ranges and corridors outside PAs. C1 [Fynn, Richard W. S.] Univ Botswana, Okavango Res Inst, Private Bag 285, Maun, Botswana. [Augustine, David J.] USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. [Peel, Michael J. S.] Agr Res Council, Anim Prod Inst, Rangeland Ecol, POB 7063, ZA-1200 Nelspruit, South Africa. [de Garine-Wichatitsky, Michel] Cirad, UPR AGIRs, Campus Int Baillarguet, F-34398 Montpellier 5, France. [de Garine-Wichatitsky, Michel] Univ Zimbabwe, Dept Biol Sci, RP PCP, POB MP 167, Harare, Zimbabwe. RP Fynn, RWS (reprint author), Univ Botswana, Okavango Res Inst, Private Bag 285, Maun, Botswana. EM rfynn@ori.ub.bw FU FSP-RenCaRe project - Ministere Francais des Affaires Etrangeres et Europeennes; ANR-SAVARID project FX We thank Justin Derner, Herve Fritz, Rob Pringle and an anonymous reviewer for their helpful comments and suggestions on earlier versions of the manuscript. RF and MdGW were supported by the FSP-RenCaRe project funded by the Ministere Francais des Affaires Etrangeres et Europeennes. MdGW was supported by the ANR-SAVARID project (CEP&S 2011). NR 85 TC 5 Z9 5 U1 16 U2 84 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD APR PY 2016 VL 53 IS 2 BP 388 EP 397 DI 10.1111/1365-2664.12591 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DE9LN UT WOS:000370959100011 ER PT J AU Zhu, QB Guan, JY Huang, M Lu, RF Mendoza, F AF Zhu, Qibing Guan, Jiyu Huang, Min Lu, Renfu Mendoza, Fernando TI Predicting bruise susceptibility of 'Golden Delicious' apples using hyperspectral scattering technique SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Apple; Bruise susceptibility; Hyperspectral scattering; Partial least squares; Nondestructive detection ID SOLUBLE SOLIDS CONTENT; FRACTURE PROPERTIES; OPTICAL-PROPERTIES; NIR SPECTROSCOPY; FRUIT FIRMNESS; HARVEST DATE; QUALITY; ABSORPTION; IMAGES; TIME AB Prediction of the susceptibility of apples to bruising can provide useful information for proper postharvest handling and storage operations. The objective of this research was to develop a nondestructive method for predicting the bruise susceptibility of apples using hyperspectral scattering technique. Spectral scattering images between 500 and 1000 nm were acquired for 300 'Golden Delicious' apples over a time period of three weeks after harvest, using a hyperspectral imaging system. Individual apples were then subjected to impact test by a pendulum ball at one of the three levels of impact energy, i.e., 1.11, 0.66, and 0.33 J. Relative mean reflectance was computed for the scattering profile of each wavelength over 10 mm scattering distance. Bruise volumes were estimated from the digital images of the bruised fruit, using a bruise volume estimation model. The bruise susceptibility of apples, determined as the ratio of bruise volumeto impact energy, ranged between 353 and 881 mm(3) J(-1) for the test apples. Bruise susceptibility was affected by the level of impact energy; higher bruise susceptibility values were obtained at low impact energy. Partial least squares (PLS) models for bruise susceptibility were developed for each impact energy level as well as for the pooled data. Better predictions of bruise susceptibility were obtained from the PLS models for each impact energy level, with the correlation coefficient of prediction or R-p = 0.848-0.919 and root mean square error of prediction or RMSEP = 32.4-50.7 mm(3) J(-1). Lower prediction results were obtained for the pooled data (R-P = 0.826 and RMSEP = 69.7 mm(3) J(-1)). This research demonstrated that hyperspectral scattering can be used for evaluating the bruise susceptibility of apples, which would be useful for postharvest handling of fruit. (C) 2015 Elsevier B.V. All rights reserved. C1 [Zhu, Qibing; Guan, Jiyu; Huang, Min] Jiangnan Univ, Minist Educ, Key Lab Adv Proc Control Light Ind, Wuxi 214122, Peoples R China. [Lu, Renfu] Michigan State Univ, ARS, USDA, Sugarbeet & Bean Res Unit, 524 S Shaw Lane,Room 224, E Lansing, MI 48824 USA. [Mendoza, Fernando] Michigan State Univ, Dept Biosyst & Agr Engn, 524 S Shaw Lane, E Lansing, MI 48824 USA. RP Zhu, QB (reprint author), Jiangnan Univ, Sch Internet Things, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China. EM zhuqib@163.com FU National Natural Science Foundation of China [61275155, 61271384]; 111 Project [B12018]; Qing Lan Project FX Qibing Zhu, Jiyu Guan and Min Huang would like to acknowledge financial support from the National Natural Science Foundation of China (grant no. 61275155 and 61271384), the 111 Project (B12018) and Qing Lan Project. NR 40 TC 3 Z9 3 U1 8 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD APR PY 2016 VL 114 BP 86 EP 94 DI 10.1016/j.postharvbio.2015.12.007 PG 9 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DE0IA UT WOS:000370307100011 ER PT J AU Sasidharan, S Torkzaban, S Bradford, SA Kookana, R Page, D Cook, PG AF Sasidharan, Salini Torkzaban, Saeed Bradford, Scott A. Kookana, Rai Page, Declan Cook, Peter G. TI Transport and retention of bacteria and viruses in biochar-amended sand SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Biochar; Porous media; E. coli bacteria; Bacteriophages MS2; PRD1; Phi X174; Straining; Attachment ID ESCHERICHIA-COLI TRANSPORT; HETEROGENEOUS POROUS-MEDIA; COLLOID FILTRATION THEORY; SECONDARY ENERGY MINIMUM; SATURATED SAND; PARTICLE-SIZE; HUMIC-ACID; NANOPARTICLE TRANSPORT; PYROLYSIS TEMPERATURE; CHEMICAL ACTIVATION AB The transport and retention of Escherichia coli and bacteriophages (PRD1, MS2 and Phi X174), as surrogates for human pathogenic bacteria and viruses, respectively, were studied in the sand that was amended with several types of biochar produced from various feedstocks. Batch and column studies were conducted to distinguish between the role of attachment and straining in microbe retention during transport. Batch experiments conducted at various solution chemistries showed negligible attachment of viruses and bacteria to biochar before or after chemical activation. At any given solution ionic strength, the attachment of viruses to sand was significantly higher than that of biochar, whereas bacteria showed no attachment to either sand or biochar. Consistent with batch results, biochar addition (10% w/w) to sand reduced virus retention in the column experiments, suggesting a potential negative impact of biochar application to soil on virus removal. In contrast, the retention of bacteria was enhanced in biochar-amended sand columns. However, elimination of the fine fraction (<60 mu m) of biochar particles in biochar-amended sand columns significantly reduced bacteria retention. Results from batch and column experiments suggest that land application of biochar may only play a role in microbe retention via straining, by alteration of pore size distribution, and not via attachment. Consequently, the particle size distribution of biochar and sediments is a more important factor than type of biochar in determining whether land application of biochar enhances or diminishes microbial retention. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sasidharan, Salini; Torkzaban, Saeed; Kookana, Rai; Page, Declan] CSIRO Land & Water, Glen Osmond, SA 5064, Australia. [Sasidharan, Salini; Cook, Peter G.] Flinders Univ S Australia, NCGRT, Adelaide, SA 5001, Australia. [Bradford, Scott A.] ARS, USDA, Salin Lab, Riverside, CA 92507 USA. RP Torkzaban, S (reprint author), CSIRO Land & Water, Glen Osmond, SA 5064, Australia. RI Page, Declan/F-2566-2011; Kookana, Rai/A-5170-2012; Sasidharan, Salini/M-9386-2015 OI Page, Declan/0000-0002-4902-3911; Kookana, Rai/0000-0002-0477-3284; Sasidharan, Salini/0000-0002-2781-363X FU National Centre for Groundwater Research and Training, an Australian Government initiative; Australian Research Council; National Water Commission; CSIRO Land and Water FX Funding for this research was provided by the National Centre for Groundwater Research and Training, an Australian Government initiative, supported by the Australian Research Council and the National Water Commission, and by CSIRO Land and Water. The work was conducted in the CSIRO Land and Water Laboratory on the Waite Campus, Adelaide, South Australia. NR 83 TC 1 Z9 1 U1 22 U2 70 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 1 PY 2016 VL 548 BP 100 EP 109 DI 10.1016/j.scitotenv.2015.12.126 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DD9LC UT WOS:000370246000012 PM 26802338 ER PT J AU Haff, R Jackson, E Gomez, J Light, D Follett, P Simmons, G Higbee, B AF Haff, Ron Jackson, Eric Gomez, Joseph Light, Doug Follett, Peter Simmons, Greg Higbee, Brad TI Building lab-scale x-ray tube based irradiators SO RADIATION PHYSICS AND CHEMISTRY LA English DT Article DE X-ray; Irradiator; Dose rate; Dose uniformity AB Here we report the use of x-ray tube based irradiators as alternatives to gamma sources for laboratory scale irradiation. Irradiators were designed with sample placement in closest possible proximity to the source, allowing high dose rates for small samples. Designs using 1000 W x-ray tubes in single tube, double tube, and four tube configurations are described, as well as various cabinet construction techniques. Relatively high dose rates were achieved for small samples, demonstrating feasibility for laboratory based irradiators for research purposes. Dose rates of 9.76, 5.45, and 1.7 Gy/min/tube were measured at the center of a 12.7 cm container of instant rice at 100 keV, 70 keV, and 40 keV, respectively. Dose uniformity varies dramatically as the distance from source to container. For 2.54 cm diameter sample containers containing adult Navel Orangeworm, dose rates of 50-60 Gy/min were measured in the four tube system. Published by Elsevier Ltd. C1 [Haff, Ron; Jackson, Eric; Gomez, Joseph; Light, Doug] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Follett, Peter] ARS, USDA, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Simmons, Greg] US Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, Ctr Plant Hlth Sci & Technol, 1636 E Alisal St, Salinas, CA 93905 USA. [Higbee, Brad] Wonderful Orchards, 6801 E Lerdo Hwy, Shafter, CA 93263 USA. RP Haff, R (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM Ron.Haff@ars.usda.gov FU [60-5325-9-651] FX This research was partially funded through an inter-agency Grant between USDA-APHIS and USDA-ARS (Agreement # 60-5325-9-651). NR 13 TC 0 Z9 0 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0969-806X J9 RADIAT PHYS CHEM JI Radiat. Phys. Chem. PD APR PY 2016 VL 121 BP 43 EP 49 DI 10.1016/j.radphyschem.2015.12.010 PG 7 WC Chemistry, Physical; Nuclear Science & Technology; Physics, Atomic, Molecular & Chemical SC Chemistry; Nuclear Science & Technology; Physics GA DD7ID UT WOS:000370096000006 ER PT J AU Dale, VH Kline, KL Buford, MA Volk, TA Smith, CT Stupak, I AF Dale, Virginia H. Kline, Keith L. Buford, Marilyn A. Volk, Timothy A. Smith, C. Tattersall Stupak, Inge TI Incorporating bioenergy into sustainable landscape designs SO RENEWABLE & SUSTAINABLE ENERGY REVIEWS LA English DT Review DE Adaptive management; Biofuel; Planning; Resource management; Scale; Stakeholder ID AGRICULTURAL RESIDUE REMOVAL; BIOMASS FEEDSTOCK PRODUCTION; BIOETHANOL SUPPLY CHAIN; DECISION-SUPPORT TOOL; FOREST MANAGEMENT; BIODIVERSITY CONSERVATION; STAKEHOLDER ENGAGEMENT; ENERGY SECURITY; MARGINAL LAND; SYSTEMS AB The paper describes an approach to landscape design that focuses on integrating bioenergy production with other components of environmental, social and economic systems. Landscape design as used here refers to a spatially explicit, collaborative plan for management of landscapes and supply chains. Landscape design can involve multiple scales and build on existing practices to reduce costs or enhance services. Appropriately applied to a specific context, landscape design can help people assess trade-offs when making choices about locations, types of feedstock, transport, refining and distribution of bioenergy products and services. The approach includes performance monitoring and reporting along the bioenergy supply chain. Examples of landscape design applied to bioenergy production systems are presented. Barriers to implementation of landscape design include high costs, the need to consider diverse land-management objectives from a wide array of stakeholders, up-front planning requirements, and the complexity and level of effort needed for successful stakeholder involvement. A landscape design process may be stymied by insufficient data or participation. An impetus for coordination is critical, and incentives may be required to engage landowners and the private sector. Hence devising and implementing landscape designs for more sustainable outcomes require clear communication of environmental, social, and economic opportunities and concerns. (C) 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Dale, Virginia H.; Kline, Keith L.] Oak Ridge Natl Lab, Div Environm Sci, Ctr Bioenergy Sustainabil, POB 2008, Oak Ridge, TN 37831 USA. [Buford, Marilyn A.] US Forest Serv Res & Dev, Washington, DC 20250 USA. [Volk, Timothy A.] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Smith, C. Tattersall] Univ Toronto, Toronto, ON, Canada. [Stupak, Inge] Univ Copenhagen, Copenhagen, Denmark. RP Dale, VH (reprint author), 1 Bethel Valley Rd, Oak Ridge, TN 37830 USA. EM dalevh@ornl.gov; klinekl@ornl.gov; mbuford@fs.fed.us; tavolk@esf.edu; tat.smith@utoronto.ca; ism@ign.ku.dk OI Kline, Keith/0000-0003-2294-1170 FU U.S. Department of Energy (DOE) under the Bioenergy Technologies Office; DOE [DE-AC05-00OR22725] FX This research was supported by the U.S. Department of Energy (DOE) under the Bioenergy Technologies Office. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for DOE under Contract DE-AC05-00OR22725. Comments by Ben Wigley, Arnaldo Walter, Camila Ortolan Fernandes de Oliveira, and Yetta Jager on an earlier draft are greatly appreciated. Erica Atkin edited the manuscript, and Gina Busby helped check references. This paper is dedicated to the memory of Al Lucier, who helped organize a workshop on "Incorporating Bioenergy in Sustainable Landscape Designs" held in March 2014 in New Bern, North Carolina, at which many of our ideas developed. NR 103 TC 6 Z9 6 U1 10 U2 38 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1364-0321 J9 RENEW SUST ENERG REV JI Renew. Sust. Energ. Rev. PD APR PY 2016 VL 56 BP 1158 EP 1171 DI 10.1016/j.rser.2015.12.038 PG 14 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA DC8IC UT WOS:000369462100090 ER PT J AU Cherubin, MR Karlen, DL Franco, ALC Tormena, CA Cerri, CEP Davies, CA Cerri, CC AF Cherubin, Mauricio R. Karlen, Douglas L. Franco, Andre L. C. Tormena, Cassio A. Cerri, Carlos E. P. Davies, Christian A. Cerri, Carlos C. TI Soil physical quality response to sugarcane expansion in Brazil SO GEODERMA LA English DT Article DE Land-use change; Soil compaction; Soil physical functions; Ethanol production ID LAND-USE CHANGE; TROPICAL SOILS; HYDRAULIC CONDUCTIVITY; ECOSYSTEM SERVICES; COMPACTION; BIOFUELS; CERRADO; IMPACTS; CARBON; PENETRATION AB Globally, the rate of land-use change (LUC) is increasing rapidly to support biofuel feedstock production. In Brazil, sugarcane (Saccharum officinarum) expansion to produce ethanol is displacing degraded pastures. Intensive mechanization for sugarcane production, could impact soil physical quality in these areas. We evaluated a typical LUC sequence (i.e., native vegetation-pasture-sugarcane) on soil physical quality at three sites in the central southern region of Brazil.-The soil physical properties evaluated through on-farm and laboratory soil analyses were: bulk density, degree of compactness, macroporosity, microporosity and total porosity, water-filled pore space, indexes of soil water storage and aeration capacity, soil resistance to penetration, field-saturated hydraulic conductivity and stability structural index. Calculations of mean weight diameter for the soil aggregates and soil physical quality ratings from a visual evaluation of soil structure (VESS) were also included in this study. From those data we defined a minimum dataset for calculating an additive soil physical quality index (SPQI). Longterm conversion from native ecosystems to pasture increased soil compaction (i.e., higher bulk density, degree of compactness and resistance to penetration values), decreased aeration porosity and water hydraulic conductivity, and consequently, created an unbalanced ratio between water- and air-filled pore space in the soil. Based on our SPQI, the soil's capacity to perform its physical functions decreased from 90% under native vegetation to 73% under pasture. Land-use change from pasture to sugarcane induced slight soil physical quality degradation, in which soil function was 68 and 56% of capacity. Overall, soil physical quality decreased under sugarcane fields, due to decreases in soil porosity, aeration and water hydraulic conductivity as well as increases in soil penetration resistance, structural degradation and erosion risk. Tillage operations performed during the sugarcane replanting (similar to 5 years) had a short-term positive effect on soil physical quality, although over time it further decreased the resistance to erosion and structural degradation. Therefore, to convert degraded pasture to sugarcane in a sustainable manner, the soils should be managed in ways that increase the soil organic matter and minimize compaction. These actions are needed to prevent further soil physical quality degradation and to improve both economic and environmental sustainability of sugarcane ethanol production. (c) 2016 Elsevier B.V. All rights reserved. C1 [Cherubin, Mauricio R.; Cerri, Carlos E. P.] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, 11 Padua Dias Ave, BR-13418900 Piracicaba, SP, Brazil. [Cherubin, Mauricio R.; Karlen, Douglas L.] USDA ARS, NLAE, 2110 Univ Blvd, Ames, IA 50011 USA. [Franco, Andre L. C.] Colorado State Univ, Dept Biol, 200 West Lake St, Ft Collins, CO 80523 USA. [Tormena, Cassio A.] Univ Estadual Maringa, Dept Agron, 5790 Colombo Ave, BR-87020900 Maringa, Parana, Brazil. [Davies, Christian A.] Shell Technol Ctr Houston, 3333 Highway 6 South, Houston, TX 77082 USA. [Cerri, Carlos C.] Univ Sao Paulo, Ctr Nucl Energy Agr, 303 Centenario Ave, BR-13400970 Piracicaba, SP, Brazil. RP Cherubin, MR (reprint author), USDA ARS, NLAE, 2110 Univ Blvd, Ames, IA 50011 USA. EM cherubin@usp.br RI Cerri, Carlos Eduardo/C-5039-2012; OI Franco, Andre/0000-0003-3294-6848 FU Sao Paulo Research Foundation (FAPESP) [2013/17581-6, 2014/16612-8, 2012/22510-8]; National Council for Scientific and Technological Development (CNPq) - Brazil; CNPq-Brazil [203376/2014-5] FX M.R.C. and A.L.C.F. thank the Sao Paulo Research Foundation (FAPESP) for research grants (Processes 2013/17581-6; 2014/16612-8 and 2012/22510-8). C.E.P.C, C.C.C and CAT thank the National Council for Scientific and Technological Development (CNPq) - Brazil for their productivity grants. C.A.T thanks CNPq-Brazil for post-doc grant (Process 203376/2014-5). We thank Felipe B. da Luz and Juliano Berghetti for assisting us during the field and laboratory work. Also, we thank Professor Dr. Alvaro P. da Silva for providing us support for laboratory analyses and Helio H.S. Franco for helping us in laboratory work. NR 59 TC 3 Z9 3 U1 18 U2 99 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD APR 1 PY 2016 VL 267 BP 156 EP 168 DI 10.1016/j.geoderma.2016.01.004 PG 13 WC Soil Science SC Agriculture GA DC8MG UT WOS:000369473100017 ER PT J AU Mitchell, JP Carter, LM Reicosky, DC Shrestha, A Pettygrove, GS Klonsky, KM Marcum, DB Chessman, D Roy, R Hogan, P Dunning, L AF Mitchell, J. P. Carter, L. M. Reicosky, D. C. Shrestha, A. Pettygrove, G. S. Klonsky, K. M. Marcum, D. B. Chessman, D. Roy, R. Hogan, P. Dunning, L. TI A history of tillage in California's Central Valley SO SOIL & TILLAGE RESEARCH LA English DT Review DE No-tillage; Conservation agriculture; Controlled traffic farming; Adoption; Cropping systems ID CONSERVATION TILLAGE; CROPPING SYSTEMS; NO-TILLAGE; AUSTRALIA; EMISSIONS; COTTON; SOIL AB The Central Valley (CV) of California is a remarkably productive agricultural region. Much of the productive capacity of the CV stems from the reliable tillage management systems that were developed beginning in the 1930s and that changed very little until the 1990s and even more dramatically in the 2000s. A variety of technologies, people and social networks have contributed to the major transformations in tillage management that have rapidly occurred during this recent time. Factors that influenced the prior slow evolution of tillage systems in the region include the need to find ways to farm with irrigation, cope with a broad range of soils, achieve high crop quality and yields to compete on world markets, expand farming operations to greater acreage, and find ways to farm with ever-increasing costs. The cost increases, recognition of the emerging concepts of conservation agriculture (CA), and the development and broader adoption of advanced irrigation systems are now spurring farmers and research organizations in the CV to overcome problems experienced with conventional tillage practices and to develop new cropping systems in the region including no-tillage and strip-tillage. Ultimately, broader adoption of conservation agriculture principles and practices in this region will stem from a diverse and complex set of motivating factors. The role of global farmer-to-farmer communication has had a major impact on this process. Ongoing targeted problem-solving efforts addressing weed, water and fertility management in conservation agriculture systems will be needed to make them more reliable and widely used. (C) 2015 Published by Elsevier B.V. C1 [Mitchell, J. P.] Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. [Carter, L. M.] ARS, USDA, Shafter Cotton Res Stn, Shafter, CA USA. [Reicosky, D. C.] ARS, USDA, Morris, MN USA. [Shrestha, A.] Calif State Univ Fresno, Dept Plant Sci, Fresno, CA 93740 USA. [Pettygrove, G. S.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Klonsky, K. M.] Univ Calif Davis, Dept Agr & Resource Econ, Davis, CA 95616 USA. [Marcum, D. B.] Univ Calif Cooperat Extens, Macarthur, CA USA. [Chessman, D.] Nat Resources Conservat Serv, USDA, Davis, CA USA. [Roy, R.] Nat Resources Conservat Serv, USDA, Fresno, CA USA. [Hogan, P.] Nat Resources Conservat Serv, USDA, Woodland, CA USA. [Dunning, L.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. RP Mitchell, JP (reprint author), Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. EM jpmitchell@ucdavis.edu NR 61 TC 2 Z9 2 U1 13 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD APR PY 2016 VL 157 BP 52 EP 64 DI 10.1016/j.still.2015.10.015 PG 13 WC Soil Science SC Agriculture GA DC4RF UT WOS:000369207300006 ER PT J AU Xu, YL Cai, NH He, B Zhang, RL Zhao, W Mao, JF Duan, AA Li, Y Woeste, K AF Xu, Yulan Cai, Nianhui He, Bin Zhang, Ruili Zhao, Wei Mao, Jianfeng Duan, Anan Li, Yue Woeste, Keith TI Germination and early seedling growth of Pinus densata Mast. provenances SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Afforestation; Conifer; Gaoshan pine; Provenance; Seed germination; Seed size; Seedling vigor ID DIPLOID HYBRID PINE; TIBETAN PLATEAU; HISTORY; FITNESS; SIZE; L.; ESTABLISHMENT; SPECIATION; DORMANCY; CANADA AB We studied seed germination and early seedling growth of Pinus densata to explore the range of variability within the species and to inform afforestation practices. Phenotypes were evaluated at a forest tree nursery under conditions that support Pinus yunnanensis, one of the presumed parental species of P. densata. Seeds were collected from 20 open-pollinated trees within each of eight autochthonous populations representing the natural distribution of P. densata in China to assess variation in germination traits and early seedling growth, and to examine the relationships among these traits. Results showed that seeds from all populations germinated and seedlings established successfully. There were significant differences among populations in 13 of 14 traits evaluated. Seed germination and early seedling growth were strongly related to seed size and seed weight. Bigger seeds germinated earlier and faster than small seeds, and seedling size was positively correlated with seed size. Some germination traits were strongly and significantly correlated with climatic variables associated with the provenance of the studied populations. Based on these observations, we conclude there were large, significant, and biologically important differences among P. densata populations in seed germination and seedling growth traits. The observed variability probably reflects a high degree of adaptive differentiation among populations that is likely to be relevant for future afforestation. C1 [Xu, Yulan; Cai, Nianhui; He, Bin; Zhang, Ruili; Duan, Anan] Southwest Forestry Univ, Coll Forestry, Key Lab Forest Resources Conservat & Use Southwes, Kunming 650224, Peoples R China. [Xu, Yulan; Mao, Jianfeng; Li, Yue] Beijing Forestry Univ, Coll Biol Sci & Biotechnol, State Engn Lab Tree Breeding, Beijing 100083, Peoples R China. [Zhao, Wei] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China. [Woeste, Keith] Purdue Univ, USDA, Forest Serv, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47906 USA. RP Xu, YL (reprint author), Southwest Forestry Univ, Coll Forestry, Key Lab Forest Resources Conservat & Use Southwes, Kunming 650224, Peoples R China.; Xu, YL (reprint author), Beijing Forestry Univ, Coll Biol Sci & Biotechnol, State Engn Lab Tree Breeding, Beijing 100083, Peoples R China. EM xvyulan@163.com FU Natural Science Foundation of China [NSFC 31070591] FX This work was supported by grants from the Natural Science Foundation of China (NSFC 31070591). NR 40 TC 0 Z9 0 U1 4 U2 39 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD APR PY 2016 VL 27 IS 2 BP 283 EP 294 DI 10.1007/s11676-015-0186-x PG 12 WC Forestry SC Forestry GA DB7MU UT WOS:000368700400007 ER PT J AU Pizarro, F Olivares, M Valenzuela, C Brito, A Weinborn, V Flores, S Arredondo, M AF Pizarro, Fernando Olivares, Manuel Valenzuela, Carolina Brito, Alex Weinborn, Valerie Flores, Sebastian Arredondo, Miguel TI The effect of proteins from animal source foods on heme iron bioavailability in humans SO FOOD CHEMISTRY LA English DT Article DE Heme iron; Animal protein; Iron bioavailability; Humans ID ABSORPTION; MECHANISMS; WOMEN; MODEL; MEAT AB Forty-five women (35-45 year) were randomly assigned to three iron (Fe) absorption sub-studies, which measured the effects of dietary animal proteins on the absorption of heme Fe. Study 1 was focused on heme, red blood cell concentrate (RBCC), hemoglobin (Hb), RBCC + beef meat; study 2 on heme, heme + fish, chicken, and beef; and study 3 on heme and heme + purified animal protein (casein, collagen, albumin). Study 1: the bioavailability of heme Fe from Hb was similar to heme only (similar to 13.0%). RBCC (25.0%) and RBCC + beef (21.3%) were found to be increased 2- and 1.6-fold, respectively, when compared with heme alone (p < 0.05). Study 2: the bioavailability from heme alone (10.3%) was reduced (p < 0.05) when it was blended with fish (7.1%) and chicken (4.9%), however it was unaffected by beef. Study 3: casein, collagen, and albumin did not affect the bioavailability of Fe. Proteins from animal source foods and their digestion products did not enhance heme Fe absorption. (C) 2015 Published by Elsevier Ltd. C1 [Pizarro, Fernando; Olivares, Manuel; Flores, Sebastian; Arredondo, Miguel] Univ Chile, Inst Nutr & Food Technol INTA, Micronutrients Lab, Santiago, Chile. [Valenzuela, Carolina] Univ Chile, Fac Veterinaty & Anim Sci, Santiago, Chile. [Brito, Alex] Univ Calif Davis, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Weinborn, Valerie] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. RP Pizarro, F (reprint author), Univ Chile, Inst Nutr & Food Technol INTA, Micronutrients Lab, Avda El Libano 5524, Santiago, Chile. EM fpizarro@inta.uchile.cl; molivare@inta.uchile.cl; cvalenzuelav@u.uchile.cl; abrito@ucdavis.edu; vweinborn@ucdavis.edu; seb.flores@postgrad.otago.ac.nz; marredon@inta.uchile.cl OI Weinborn, Valerie/0000-0001-9560-4955 FU FONDECYT, Chile [1061060] FX F.P and M.O designed the study, F.P., M.O., V.W, and S.F. conducted the research, all authors analyzed data, C.V. and A.B assisted with data interpretation. F.P, A.B and C.V wrote the paper, and F.P. had primary responsibility for the final content. All authors read and approved the final manuscript. We would like to thank Sotiris Chaniotakis for editing this manuscript. This study was supported by: FONDECYT 1061060, Chile. NR 39 TC 3 Z9 4 U1 2 U2 49 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD APR 1 PY 2016 VL 196 BP 733 EP 738 DI 10.1016/j.foodchem.2015.10.012 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CY2FI UT WOS:000366223800091 PM 26593548 ER PT J AU Tchouassi, DP Okiro, ROK Sang, R Cohnstaedt, LW McVey, DS Torto, B AF Tchouassi, David P. Okiro, Robinson O. K. Sang, Rosemary Cohnstaedt, Lee W. McVey, David Scott Torto, Baldwyn TI Mosquito host choices on livestock amplifiers of Rift Valley fever virus in Kenya SO PARASITES & VECTORS LA English DT Article DE Attractancy; Engorgement rate; RVF livestock amplifiers; Enclosure trap; Surveillance ID BLOOD MEAL ANALYSIS; VECTOR COMPETENCE; CULEX-ZOMBAENSIS; MALARIA VECTORS; LIGHT TRAPS; TRANSMISSION; PREFERENCE; CULICIDAE; DISEASE; DIPTERA AB Background: Animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. However, these parameters remain poorly understood for mosquito vectors of the Rift Valley fever (RVF), an arboviral disease, and for a community of mosquitoes. Methods: Using three known livestock amplifiers of RVF virus including sheep, goat and cattle as bait in enclosure traps, we investigated the host-feeding patterns for a community of mosquitoes in Naivasha, an endemic area of Rift Valley fever (RVF), in a longitudinal study for six months (June-November 2015). We estimated the incidence rate ratios (IRR) where mosquitoes chose cow over the other livestock hosts by comparing their attraction (total number collected) and engorgement rate (proportion freshly blood-fed) on these hosts. Results: Overall, significant differences were observed in host preference parameters for attraction (F-2,F-15 = 4.1314, P = 0.037) and engorgement (F-2,F-15 = 6.24, P = 0.01) with cow consistently attracting about 3-fold as many mosquitoes as those engorged on sheep (attraction: IRR = 2.9, 95 % CI 1.24-7.96; engorgement: IRR = 3.2, 95 % CI = 1.38-7.38) or goat (attraction: IRR = 2.7, 95 % CI 1.18-7.16; engorgement: IRR = 3.28, 95 % CI 1.47-7.53). However, there was no difference between the attraction elicited by sheep and goat (IRR = 1.08; 95 % CI 0.35-3.33 or engorgement rate (IRR = 0.96, 95 % CI 0.36-2.57). Conclusion: Despite the overall attractive pattern to feed preferentially on cows, the engorgement rate was clearly independent of the number attracted for certain mosquito species, notably among the flood water Aedes spp., largely incriminated previously as primary vectors of RVF. Our findings suggest that insecticide treated cattle (ITC) can be exploited in enclosure traps as contact bait in the monitoring and control of disease-causing mosquitoes in RVF endemic areas. C1 [Tchouassi, David P.; Okiro, Robinson O. K.; Sang, Rosemary; Torto, Baldwyn] Int Ctr Insect Physiol & Ecol, POB 30772-00100, Nairobi, Kenya. [Sang, Rosemary] Kenya Med Res Inst KEMRI, Ctr Virus Res, POB 54840-00200, Nairobi, Kenya. [Cohnstaedt, Lee W.; McVey, David Scott] USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, 515 Coll Ave, Manhattan, KS 66502 USA. RP Tchouassi, DP (reprint author), Int Ctr Insect Physiol & Ecol, POB 30772-00100, Nairobi, Kenya. EM dtchouassi@icipe.org FU USDA-ARS [SCA-58-5430-4-013F]; icipe FX We thank Julia Wanjiru of the International Centre of Insect Physiology and Ecology (icipe) for support in mosquito sorting, as well as Samwel Arum and Francis Mulwa (icipe) for occasional assistance in mosquito identification. This project was partly funded by the USDA-ARS (SCA-58-5430-4-013F) and core funds from icipe. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 0 Z9 0 U1 6 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD MAR 31 PY 2016 VL 9 AR 184 DI 10.1186/s13071-016-1473-x PG 8 WC Parasitology SC Parasitology GA DI4ZP UT WOS:000373508200001 PM 27036889 ER PT J AU Kim, SY Bender, KW Walker, BJ Zielinski, RE Spalding, MH Ort, DR Huber, SC AF Kim, Sang Y. Bender, Kyle W. Walker, Berkley J. Zielinski, Raymond E. Spalding, Martin H. Ort, Donald R. Huber, Steven C. TI The Plastid Casein Kinase 2 Phosphorylates Rubisco Activase at the Thr-78 Site but Is Not Essential for Regulation of Rubisco Activation State SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Rubisco; Rubisco activase; redox regulation; protein phosphorylation; modification-specific antibodies ID PHOSPHOPROTEOME PROFILING REVEALS; DISULFIDE BOND FORMATION; MUSTARD SINAPIS-ALBA; ARABIDOPSIS-THALIANA; PROTEIN-KINASE; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE; TRANSCRIPTION KINASE; TRANSGENIC TOBACCO; REDOX REGULATION; REDUCED AMOUNTS AB Rubisco activase (RCA) is essential for the activation of Rubisco, the carboxylating enzyme of photosynthesis. In Arabidopsis, RCA is composed of a large RCA alpha and small RCA beta isoform that are formed by alternative splicing of a single gene (At2g39730). The activity of Rubisco is controlled in response to changes in irradiance by regulation of RCA activity, which is known to involve a redox-sensitive disulfide bond located in the carboxy-terminal extension of the RCA alpha subunit. Additionally, phosphorylation of RCA threonine-78 (Thr-78) has been reported to occur in the dark suggesting that phosphorylation may also be associated with dark-inactivation of RCA and deactivation of Rubisco. In the present study, we developed site-specific antibodies to monitor phosphorylation of RCA at the Thr-78 site and used non-reducing SDS-PAGE to monitor the redox status of the RCA alpha subunit. By immunoblotting, phosphorylation of both RCA isoforms occurred at low light and in the dark and feeding peroxide or DTT to leaf segments indicated that redox status of the chloroplast stroma was a critical factor controlling RCA phosphorylation. Use of a knockout mutant identified the plastid-targeted casein kinase 2 (cpCK2 alpha) as the major protein kinase involved in RCA phosphorylation. Studies with recombinant cpCK2 alpha and synthetic peptide substrates identified acidic residues at the -1, +2, and +3 positions surrounding Thr-78 as strong positive recognition elements. The cpck2 knockout mutant had strongly reduced phosphorylation at the Thr-78 site but was similar to wild type plants in terms of induction kinetics of photosynthesis following transfer from darkness or low light to high light, suggesting that if phosphorylation of RCA Thr-78 plays a direct role it would be redundant to redox regulation for control of Rubisco activation state under normal conditions. C1 [Kim, Sang Y.; Walker, Berkley J.; Ort, Donald R.; Huber, Steven C.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. [Kim, Sang Y.; Bender, Kyle W.; Zielinski, Raymond E.; Ort, Donald R.; Huber, Steven C.] Univ Illinois Champaign Urbana, Plant Biol, Urbana, IL USA. [Walker, Berkley J.; Ort, Donald R.] Carl R Woese Inst Genom Biol, Urbana, IL USA. [Spalding, Martin H.] Iowa State Univ, Genet Dev & Cell Biol, Ames, IA USA. RP Huber, SC (reprint author), ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA.; Huber, SC (reprint author), Univ Illinois Champaign Urbana, Plant Biol, Urbana, IL USA. EM schuber1@illinois.edu OI Bender, Kyle/0000-0002-1805-8097 FU USDA-ARS; NIFA-AFRI [58-5012-5-025]; Iowa State University; Crop Bioengineering Consortium FX Support was provided by USDA-ARS, NIFA-AFRI grant 58-5012-5-025, and Iowa State University, Crop Bioengineering Consortium. NR 41 TC 3 Z9 3 U1 4 U2 11 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 31 PY 2016 VL 7 AR 404 DI 10.3389/fpls.2016.00404 PG 14 WC Plant Sciences SC Plant Sciences GA DI1NR UT WOS:000373263300001 PM 27064346 ER PT J AU King, BA Shellie, KC AF King, B. A. Shellie, K. C. TI Evaluation of neural network modeling to predict non-water-stressed leaf temperature in wine grape for calculation of crop water stress index SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Canopy temperature; Irrigation management; Water stress ID VITIS-VINIFERA L.; DEFICIT IRRIGATION; ESTIMATING EVAPOTRANSPIRATION; CABERNET-SAUVIGNON; CANOPY TEMPERATURE; BERRY COMPOSITION; VISIBLE IMAGERY; THERMAL IMAGERY; YIELD; FIELD AB Precision irrigation management of wine grape requires a reliable method to easily quantify and monitor vine water status to allow effective manipulation of plant water stress in response to water demand, cultivar management and producer objective. Mild to moderate water stress is desirable in wine grape in determined phenological periods for controlling vine vigor and optimizing fruit yield and quality according to producer preferences and objectives. The traditional leaf temperature based crop water stress index (CWSI) for monitoring plant water status has not been widely used for irrigated crops in general partly because of the need to know well-watered and non-transpiring leaf temperatures under identical environmental conditions. In this study, leaf temperature of vines irrigated at rates of 35, 70 or 100% of estimated evapotranspiration demand (ETc) under warm, semiarid field conditions in southwestern Idaho USA was monitored from berry development through fruit harvest in 2013 and 2014. Neural network (NN) models were developed based on meteorological measurements to predict well-watered leaf temperature of wine grape cultivars 'Syrah' and 'Malbec' (Vitis vinifera L). Input variables for the cultivar specific NN models with lowest mean squared error were 15-min average values for air temperature, relative humidity, solar radiation and wind speed collected within 90 min of solar noon (13:00 and 15:00 MDT). Correlation coefficients between NN predicted and measured well-watered leaf temperature were 0.93 and 0.89 for 'Syrah' and 'Malbec', respectively. Mean squared error and mean average error for the NN models were 1.07 and 0.82 degrees C for 'Syrah' and 1.30, and 0.98 degrees C for 'Malbec', respectively. The NN models predicted well-watered leaf temperature with significantly less variability than traditional multiple linear regression using the same input variables. Non-transpiring leaf temperature was estimated as air temperature plus 15 degrees C based on maximum temperatures measured for vines irrigated at 35% (ETc). Daily mean CWSI calculated using NN estimated well-watered leaf temperatures between 13:00 and 15:00 MDT and air temperature plus 15 degrees C for non-transpiring leaf temperature consistently differentiated between deficit irrigation amounts, irrigation events, and rainfall. The methodology used to calculate a daily CWSI for wine grape in this study provided a daily indicator of vine water status that could be automated for use as a decision-support tool in a precision irrigation system. Published by Elsevier B.V. C1 [King, B. A.] USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. [Shellie, K. C.] USDA ARS, Hort Crops Res Unit Worksite, 29603 Univ Idaho Lane, Parma, ID 83660 USA. RP King, BA (reprint author), USDA ARS, Northwest Irrigat & Soils Res Lab, 3793 N 3600 E, Kimberly, ID 83341 USA. EM brad.king@ars.usda.gov; Krista.shellie@ars.usda.gov NR 47 TC 1 Z9 1 U1 7 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD MAR 31 PY 2016 VL 167 BP 38 EP 52 DI 10.1016/j.agwat.2015.12.009 PG 15 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DE2KO UT WOS:000370456400005 ER PT J AU Jusino, MA Lindner, DL Banik, MT Rose, KR Walters, JR AF Jusino, Michelle A. Lindner, Daniel L. Banik, Mark T. Rose, Kevin R. Walters, Jeffrey R. TI Experimental evidence of a symbiosis between red-cockaded woodpeckers and fungi SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE cavity excavator; cavity nester; fungal communities; multipartite symbiosis; Picoides borealis; wood decay fungi ID CAVITY-NESTING BIRDS; MIGRATORY BIRDS; HEART ROT; TREES; DIVERSITY; MUTUALISM; SELECTION; WEBS; CONSERVATION; COMMUNITIES AB Primary cavity excavators, such as woodpeckers, are ecosystem engineers in many systems. Associations between cavity excavators and fungi have long been hypothesized to facilitate cavity excavation, but these relationships have not been experimentally verified. Fungi may help excavators by softening wood, while excavators may facilitate fungal dispersal. Here we demonstrate that excavators facilitate fungal dispersal and thus we report the first experimental evidence of a symbiosis between fungi and a cavity excavator, the red-cockaded woodpecker (RCW, Picoides borealis). Swab samples of birds showed that RCWs carry fungal communities similar to those found in their completed excavations. A 26-month field experiment using human-made aseptically drilled excavations in live trees, half of which were inaccessible to RCWs, demonstrated that RCWs directly alter fungal colonization and community composition. Experimental excavations that were accessible to RCWs contained fungal communities similar to natural RCW excavations, whereas inaccessible experimental excavations contained significantly different fungal communities. Our work demonstrates a complex symbiosis between cavity excavators and communities of fungi, with implications for forest ecology, wildlife management, and conservation. C1 [Jusino, Michelle A.; Lindner, Daniel L.; Banik, Mark T.] US Forest Serv, Ctr Forest Mycol Res, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Jusino, Michelle A.; Rose, Kevin R.; Walters, Jeffrey R.] Virginia Tech, Dept Biol Sci, 1405 Perry St,2125 Derring Hall, Blacksburg, VA 24061 USA. [Rose, Kevin R.] Virginia Dept Game & Inland Fisheries, 1320 Belman Rd, Fredericksburg, VA 22401 USA. RP Jusino, MA (reprint author), US Forest Serv, Ctr Forest Mycol Res, One Gifford Pinchot Dr, Madison, WI 53726 USA.; Jusino, MA (reprint author), Virginia Tech, Dept Biol Sci, 1405 Perry St,2125 Derring Hall, Blacksburg, VA 24061 USA. EM michellejusino@gmail.com FU US Department of Defense, Marine Corps Base Camp Lejeune; Harold H. Bailey fund at Virginia Tech; US Forest Service, Northern Research Station; American Ornithologists' Union; Mycological Society of America; Society for Integrative and Comparative Biology; Virginia Tech Graduate Research and Development Program; US Department of Defense, Strategic Environmental Research and Development Program [RC-1471, RC-1413] FX Funding for fieldwork was provided by the US Department of Defense, Marine Corps Base Camp Lejeune. Funding for the analysis of samples was provided by the Harold H. Bailey fund at Virginia Tech and the US Forest Service, Northern Research Station. Further funding for this work was provided by the American Ornithologists' Union, the Mycological Society of America, the Society for Integrative and Comparative Biology and the Virginia Tech Graduate Research and Development Program. Further support for M.A.J. was provided by the US Department of Defense, Strategic Environmental Research and Development Program (RC-1471 and RC-1413, Defense Coastal/Estuarine Research Program). NR 43 TC 0 Z9 0 U1 22 U2 32 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD MAR 30 PY 2016 VL 283 IS 1827 AR 20160106 DI 10.1098/rspb.2016.0106 PG 7 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DL6EM UT WOS:000375732500012 PM 27009222 ER PT J AU Glenn, DM AF Glenn, D. M. TI Effect of highly processed calcined kaolin residues on apple productivity and quality SO SCIENTIA HORTICULTURAE LA English DT Article DE Malus x domestica; Microbial populations; Ozone; Light interception ID PARTICLE FILM APPLICATION; WATER-USE EFFICIENCY; EMPIRE APPLE; FRUIT-QUALITY; DIPTERA TEPHRITIDAE; GAS-EXCHANGE; YIELD; OZONE; LEAF; PHOTOSYNTHESIS AB Calcined kaolin-based particle films (PKPF) are effective in reducing insect, heat, photosynthetically active radiation (PAR) and ultraviolet radiation (UV) stress in plants due to the reflective nature of the heat treated particles. The purpose of this study was to determine the effects of PKFP treatments applied at moderate (3%) and high rates (12% w/v) on apple physiology, yield and quality and the interaction with ambient ozone levels for a 6 year period. Fruit mass was increased by applications of PKPF at 3% and 12% in 4 of 6 years in non-irrigated treatments and in 2 of 6 years in irrigated treatments. Red fruit color was increased (lower hue angle) over the control in 5 of 6 years and in 2 of 5 years the 12% PKPF had improved red color over both the 0% and 3% PKPF non-irrigated treatments. PAR interception over 6 years, adjusted for LAI, was greatest for the control, least for the 12% PKPF, and 3% PKPF was intermediate. Reduced PAR interception below the canopy was likely due to greater PAR reflection within the canopy. Whole canopy temperature was significantly higher for the control (26 degrees C) and lower for the 3% and 12% PKPF treatments (24 degrees C). Fruit mass increased in the PKPF treatments, relative to the irrigated control treatment (%), with increasing maximum ozone levels during May. In laboratory studies, ozone degradation was greatest for alfalfa, least for calcined kaolin and the addition of alfalfa to calcined kaolin increased degradation proportionately. The present study confirms that calcined kaolin is an effective catalyst for ozone degradation and organic materials, such as alfalfa, are even more effective, due to the direct reaction with carbon molecules. Whole canopy gas exchange measurements (1000-1600 HR) over a 10 day period in 2011 indicated that the 2.7% PKPF + 0.3% alfalfa dust consistently had higher photosynthesis, 0.3% alfalfa was intermediate, while the control and 3% PKPF had the lowest photosynthesis rate. Whole canopy ozone degradation indicated that the 2.7% PKPF + 0.3% alfalfa dust consistently had the greatest ozone degradation, 3% PKPF and 0.3% alfalfa were intermediate and the untreated control had the least ozone degradation. There is evidence that the 0.3% alfalfa stimulated a small amount of microbial growth on the leaf surface while the 2.7% PKPF + 0.3% alfalfa stimulated a much greater amount of microbial growth. This synergistic effect may be due to the increased surface area in the particle film providing habitat for microbial populations and the reflection of UV by the particles that can be deleterious to microbial growth within that habitat. Data support the concept that chronic ozone damage is moderated to a significant degree by the use of surface treatments that catalyze ozone degradation in a particle film. The use of PKPF may be one tool to mitigate not only increased ozone stress but also heat stress from increased growing season temperatures in the future. Published by Elsevier B.V. C1 [Glenn, D. M.] USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov FU USDA-ARS; TKI Nova Source, Inc. Phoenix, AZ FX This research was supported in part by a cooperative research and development agreement between USDA-ARS and TKI Nova Source, Inc. Phoenix, AZ. NR 53 TC 1 Z9 1 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD MAR 30 PY 2016 VL 201 BP 101 EP 108 DI 10.1016/j.scienta.2016.01.035 PG 8 WC Horticulture SC Agriculture GA DI5LA UT WOS:000373539300013 ER PT J AU Bowman, KD McCollum, G Albrecht, U AF Bowman, Kim D. McCollum, Greg Albrecht, Ute TI Performance of 'Valencia' orange (Citrus sinensis [L.] Osbeck) on 17 rootstocks in a trial severely affected by huanglongbing SO SCIENTIA HORTICULTURAE LA English DT Article DE Sweet orange; Citrus greening; Fruit breeding ID CANDIDATUS LIBERIBACTER ASIATICUS; CENTRAL FLORIDA; SWEET ORANGE; TOLERANCE; DISEASE; PCR AB 'Valencia' orange (Citrus sinensis [L.] Osbeck) was grown on 17 rootstocks through seven years of age and the first four harvest seasons in a central Florida field trial severely affected by huanglongbing (HLB) disease. All trees in the trial had HLB symptoms and were shown by PCR to be infected with Candidatus Liberibacter asiaticus (Las). Large differences were noted between rootstocks for many metrics examined, including yield, fruit quality, and tree size. Highest yields in the trial were on US-942 rootstock, which was significantly more productive than trees on the common commercial rootstocks Carrizo, Kuharske, Cleopatra, and Kinkoji. Other new hybrid rootstocks also performed well in this trial strongly affected by HLB, including the rootstock US-1516, which had the second highest cumulative yield, best tree health rating, and lowest number of trees lost due to HLB damage. Comparison of tree performance in this trial with a similar trial conducted prior to the HLB epidemic, allows us to estimate that the disease resulted in a 33% reduction in yield and 21% reduction in tree growth through seven years of age. Use of a tolerant rootstock is suggested as an effective means of ameliorating crop losses to HLB. Published by Elsevier B.V. C1 [Bowman, Kim D.; McCollum, Greg; Albrecht, Ute] ARS, US Hort Res Lab, USDA, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. RP Bowman, KD (reprint author), ARS, US Hort Res Lab, USDA, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM kim.bowman@ars.usda.gov FU Florida Citrus Research and Development Foundation FX We thank the cooperator, David Wheeler, for his willingness to establish and care for the trial, and for his assistance in collecting data. We thank Emily Domagtoy, Diane Helseth, Sailindra Patel, Kerry Worton, James Salvatore, and Wayne Brown for technical assistance. This research was supported in part by grants from the Florida Citrus Research and Development Foundation. Mention of a trademark, warranty, proprietary product, or vendor does not imply an approval to the exclusion of other products or vendors that also may be suitable. NR 20 TC 3 Z9 3 U1 16 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD MAR 30 PY 2016 VL 201 BP 355 EP 361 DI 10.1016/j.scienta.2016.01.019 PG 7 WC Horticulture SC Agriculture GA DI5LA UT WOS:000373539300043 ER PT J AU Wang, QX Gao, SD Wang, D Spokas, K Cao, AC Yan, DD AF Wang, Qiuxia Gao, Suduan Wang, Dong Spokas, Kurt Cao, Aocheng Yan, Dongdong TI Mechanisms for 1,3-Dichioropropene Dissipation in Biochar-Amended Soils SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article; Proceedings Paper CT 1st International Symposium on Phytochemicals in Medicine and Food (ISPMF) CY JUN 26-29, 2015 CL Shanghai Normal Univ, Shanghai, PEOPLES R CHINA SP Phytochem Soc Europe, Phytochem Soc Asia, Macau Univ Sci & Technol, Fujian Agr & Forestry Univ, Guizhou Med Univ, Yancheng Inst Technol, Beijing Normal Univ, Fudan Univ HO Shanghai Normal Univ DE biochar; fumigant; chemical degradation; adsorption isotherm ID DUBININ-RADUSHKEVICH EQUATION; FIELD-SCALE EMISSIONS; 1,3-DICHLOROPROPENE; TEMPERATURE; DEGRADATION; ADSORPTION; SORPTION; CARBON; WATER; VOLATILIZATION AB Biochar, which is organic material heated under a limited supply of oxygen, has the potential to reduce fumigant emissions when incorporated in the soil, but the mechanisms are not fully understood. The objective of this study was to determine the effects of biochar properties, amendment rate, soil microbe, moisture, temperature, and soil type on the fate of 1,3-clichloropropene (1,3-D) isomers in laboratory incubation experiments by assessing the 1,3-D degradation rate and adsorption capacity. 1,3-D dissipation rates were significantly reduced due to strong adsorption by biochar, which was also strongly affected by biochar type. Following a 1% biochar amendment, the half-lives of 1,3-D in soil were increased 2.5-35 times. The half-lives of 1,3-D in soil were strongly affected by soil moisture, temperature, and amendment rate. The effects of sterilization on 1,34) degradation were much smaller in biochar-amended soils than in nonsterilized soils, which suggests the importance of abiotic pathways with biochar's presence: Dissipation of 1,3-D in biochar was divided into Adsorption (49-93%) and, chemical degradation pathways. Biochar properties, such as specific surface area (SSA), pH, water content, carbon content, and feedstock, all appeared to affect 1,3-D dissipation with potentially complex interactions. The biochar (air-dry) water content was highly correlated with 1,3-D adsorption capacity and thus can serve as an important predictor for fumigant mitigation use. The fate of the adsorbed fumigant onto biochar requires further examination on potential long-term environmental impacts before guidelines for biochar as a field practice to control fumigant emissions can be formulated. C1 [Wang, Qiuxia; Cao, Aocheng; Yan, Dongdong] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. [Gao, Suduan; Wang, Dong] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93646 USA. [Spokas, Kurt] Univ Minnesota, ARS, USDA, Soil & Water Management Unit, S531 Soils Bldg,1529 Gortner Ave, St Paul, MN 55108 USA. RP Wang, QX (reprint author), Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. EM qxwang@ippcaas.cn RI Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 FU Almond Board of California, USA; Beijing Nova program [2010B064] FX Funding support to this research was partially provided by the Almond Board of California, USA, and partially provided by Beijing Nova program (No. 2010B064). NR 42 TC 0 Z9 0 U1 7 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 30 PY 2016 VL 64 IS 12 BP 2531 EP 2540 DI 10.1021/acs.jafc.5b04941 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DI5DM UT WOS:000373519100013 PM 26954066 ER PT J AU Castro-Gomes, T Corrotte, M Tam, C Andrews, NW AF Castro-Gomes, Thiago Corrotte, Matthias Tam, Christina Andrews, Norma W. TI Plasma Membrane Repair Is Regulated Extracellularly by Proteases Released from Lysosomes SO PLOS ONE LA English DT Article ID HUMAN ACID SPHINGOMYELINASE; ACTIVITY-BASED PROBES; CATHEPSIN-D; MUSCULAR-DYSTROPHY; CYSTEINE CATHEPSINS; EXOCYTOSIS; DYSFERLIN; CELLS; ENDOCYTOSIS; PROTEINS AB Eukaryotic cells rapidly repair wounds on their plasma membrane. Resealing is Ca(2+-)dependent, and involves exocytosis of lysosomes followed by massive endocytosis. Extracellular activity of the lysosomal enzyme acid sphingomyelinase was previously shown to promote endocytosis and wound removal. However, whether lysosomal proteases released during cell injury participate in resealing is unknown. Here we show that lysosomal proteases regulate plasma membrane repair. Extracellular proteolysis is detected shortly after cell wounding, and inhibition of this process blocks repair. Conversely, surface protein degradation facilitates plasma membrane resealing. The abundant lysosomal cysteine proteases cathepsin B and L, known to proteolytically remodel the extracellular matrix, are rapidly released upon cell injury and are required for efficient plasma membrane repair. In contrast, inhibition of aspartyl proteases or RNAi-mediated silencing of the lysosomal aspartyl protease cathepsin D enhances resealing, an effect associated with the accumulation of active acid sphingomyelinase on the cell surface. Thus, secreted lysosomal cysteine proteases may promote repair by facilitating membrane access of lysosomal acid sphingomyelinase, which promotes wound removal and is subsequently downregulated extracellularly by a process involving cathepsin D. C1 [Castro-Gomes, Thiago; Corrotte, Matthias; Tam, Christina; Andrews, Norma W.] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. [Tam, Christina] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Andrews, NW (reprint author), Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. EM andrewsn@umd.edu FU National Institutes of Health [GM064625]; Conselho Nacional de Pesquisas, Brazil FX This work was supported by at the National Institutes of Health grant GM064625 and a fellowship from the Conselho Nacional de Pesquisas, Brazil. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 2 Z9 2 U1 2 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 30 PY 2016 VL 11 IS 3 AR e0152583 DI 10.1371/journal.pone.0152583 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH9LB UT WOS:000373116500053 PM 27028538 ER PT J AU Smart, M Pettis, J Rice, N Browning, Z Spivak, M AF Smart, Matthew Pettis, Jeff Rice, Nathan Browning, Zac Spivak, Marla TI Linking Measures of Colony and Individual Honey Bee Health to Survival among Apiaries Exposed to Varying Agricultural Land Use SO PLOS ONE LA English DT Article ID VARROA-DESTRUCTOR ACARI; APIS-MELLIFERA L.; ANTIMICROBIAL PEPTIDES; COLLAPSE DISORDER; POLLEN NUTRITION; IMMUNE FUNCTION; PROTEIN COSTS; TRADE-OFF; LIFE-SPAN; INSECT AB We previously characterized and quantified the influence of land use on survival and productivity of colonies positioned in six apiaries and found that colonies in apiaries surrounded by more land in uncultivated forage experienced greater annual survival, and generally more honey production. Here, detailed metrics of honey bee health were assessed over three years in colonies positioned in the same six apiaries. The colonies were located in North Dakota during the summer months and were transported to California for almond pollination every winter. Our aim was to identify relationships among measures of colony and individual bee health that impacted and predicted overwintering survival of colonies. We tested the hypothesis that colonies in apiaries surrounded by more favorable land use conditions would experience improved health. We modeled colony and individual bee health indices at a critical time point (autumn, prior to overwintering) and related them to eventual spring survival for California almond pollination. Colony measures that predicted overwintering apiary survival included the amount of pollen collected, brood production, and Varroa destructor mite levels. At the individual bee level, expression of vitellogenin, defensin1, and lysozyme2 were important markers of overwinter survival. This study is a novel first step toward identifying pertinent physiological responses in honey bees that result from their positioning near varying landscape features in intensive agricultural environments. C1 [Smart, Matthew; Spivak, Marla] Univ Minnesota, Dept Entomol, St Paul, MN USA. [Pettis, Jeff; Rice, Nathan] ARS, USDA, Bee Res Lab, Beltsville, MD USA. [Browning, Zac] Brownings Honey Co, Jamestown, ND USA. RP Smart, M (reprint author), Univ Minnesota, Dept Entomol, St Paul, MN USA. EM msmart@usgs.gov NR 78 TC 5 Z9 5 U1 12 U2 33 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 30 PY 2016 VL 11 IS 3 AR e0152685 DI 10.1371/journal.pone.0152685 PG 28 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH9LB UT WOS:000373116500063 PM 27027871 ER PT J AU Huang, HQ Xie, ZH Yokoyama, W Yu, LL Wang, TTY AF Huang, Haiqiu Xie, Zhuohong Yokoyama, Wallace Yu, Liangli Wang, Thomas T. Y. TI Identification of liver CYP51 as a gene responsive to circulating cholesterol in a hamster model SO JOURNAL OF NUTRITIONAL SCIENCE LA English DT Article DE Circulating cholesterol; CYP51; Hamsters; Hypercholesterolaemia ID LOWER PLASMA-CHOLESTEROL; LIPID-METABOLISM; ESTER TRANSFER; BILE-ACIDS; CHOLESTYRAMINE; EXPRESSION; 7-ALPHA-HYDROXYLASE; LIPOPROTEINS; RECEPTOR; STEROL AB Hypercholesterolaemia is a risk factor for CVD, which is a leading cause of death in industrialised societies. The biosynthetic pathways for cholesterol metabolism are well understood; however, the regulation of circulating cholesterol by diet is still not fully elucidated. The present study aimed to gain more comprehensive understanding of the relationship between circulating cholesterol levels and molecular effects in target tissues using the hamster model. Male golden Syrian hamsters were fed with chow or diets containing 36 % energy from fat with or without 1 % cholesteyramine (CA) as a modulator of circulating cholesterol levels for 35 d. It was revealed that the expression of lanosterol 14 alpha-demethylase (CYP51) instead of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase mRNA expression was responsive to circulating cholesterol in hamsters fed hypercholesterolaemic diets. The high-fat diet increased circulating cholesterol and down-regulated CYP51, but not HMG-CoA reductase. The CA diet decreased cholesterol and increased CYP51 expression, but HMG-CoA reductase expression was not affected. The high-fat diet and CA diet altered the expression level of cholesterol, bile acids and lipid metabolism-associated genes (LDL receptor, cholesterol 7 alpha-hydroxylase (CYP7A1), liver X receptor (LXR) alpha, and ATP-binding cassette subfamily G member 5/8 (ABCG5/8)) in the liver, which were significantly correlated with circulating cholesterol levels. Correlation analysis also showed that circulating cholesterol levels were regulated by LXR/retinoid X receptor and PPAR pathways in the liver. Using the hamster model, the present study provided additional molecular insights into the influence of circulating cholesterol on hepatic cholesterol metabolism pathways during hypercholesterolaemia. C1 [Huang, Haiqiu; Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, USDA, Beltsville, MD 20705 USA. [Xie, Zhuohong] Int Chem Testing, Milford, MA 01757 USA. [Yokoyama, Wallace] ARS, Proc Foods Res, USDA, Albany, CA 94710 USA. [Yu, Liangli] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Wang, TTY (reprint author), ARS, Diet Genom & Immunol Lab, USDA, Beltsville, MD 20705 USA.; Yu, LL (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM lyu5@umd.edu; Tom.Wang@ars.usda.gov RI Huang, Haiqiu/J-8856-2012 NR 43 TC 0 Z9 0 U1 1 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 2048-6790 J9 J NUTR SCI JI J. Nutr. Sci. PD MAR 30 PY 2016 VL 5 AR e16 DI 10.1017/jns.2016.3 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DI1TX UT WOS:000373279800001 PM 27110359 ER PT J AU Rimando, AM Khan, SI Mizuno, CS Ren, G Mathews, ST Kim, H Yokoyama, W AF Rimando, Agnes M. Khan, Shabana I. Mizuno, Cassia S. Ren, Guang Mathews, Suresh T. Kim, Hyunsook Yokoyama, Wallace TI Evaluation of PPAR alpha activation by known blueberry constituents SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE PPAR alpha; blueberry; anthocyanin; pterostilbene; resveratrol; catechin ID PERFORMANCE LIQUID-CHROMATOGRAPHY; RECEPTOR-ALPHA; ENRICHED DIET; ANTHOCYANINS; PTEROSTILBENE; RESVERATROL; CHOLESTEROL; PLASMA; SPECTROMETRY; METABOLISM AB BACKGROUND: Anthocyanins are known to have hypolipidemic properties. It was deemed necessary to determine whether major blueberry anthocyanins and catechins are ligands for the transcription factor peroxisome proliferator activated receptor alpha isoform (PPAR alpha), and compare activation with known PPAR alpha agonistic constituents, pterostilbene and resveratrol. It was also considered important to investigate the effect of pterostilbene on PPAR alpha gene expression, and relate results with hepatic mRNA PPAR alpha expression up-regulation observed previously in hamsters fed a diet supplemented with blueberry peels extract (BBX). RESULTS: The anthocyanins and catechins did not activate PPAR alpha. Only pterostilbene exhibited a dose-dependent activation of PPAR alpha in H4IIEC3 cells. The resveratrol responses were lower than those of pterostilbene. Pterostilbene significantly and dose-dependently (at 10, 20 and 50 mu mol L-1) increased PPAR alpha gene expression and the effect at 10 mu mol L-1 was greater than 100 and 200 mu mol L-1 of fenofibrate. Analysis of BBX showed levels of pterostilbene and resveratrol at 418 and 2381 ng g(-1), respectively. CONCLUSION: Anthocyanins and catechins do not appear to contribute to the up-regulation of hepatic PPAR alpha expression observed in hamsters. While pterostilbene and resveratrol demonstrated PPAR alpha. activation, their levels in BBX do not seem to be at efficacious concentrations. These stilbenes may contribute to the up-regulation of PPAR alpha expression by acting synergistically with each other or with other constituents in BBX. (C) 2015 Society of Chemical Industry C1 [Mizuno, Cassia S.] Univ New England, Coll Pharm, 716 Stevens Ave, Portland, ME 04103 USA. [Ren, Guang] Univ Alabama Birmingham, Sch Med, Div Endocrinol Diabet & Metab, 7720 2nd Ave S, Birmingham, AL 35292 USA. [Kim, Hyunsook] Konkuk Univ, Dept Physiol, Coll Vet Med, Seoul, South Korea. [Rimando, Agnes M.; Mizuno, Cassia S.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Khan, Shabana I.] Univ Mississippi, Natl Ctr Nat Prod Res, Sch Pharm, University, MS 38677 USA. [Ren, Guang; Mathews, Suresh T.] Auburn Univ, Dept Nutr Dietet & Hospitality Management, 260 Lem Morrison Dr, Auburn, AL 36849 USA. [Kim, Hyunsook; Yokoyama, Wallace] ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Rimando, AM (reprint author), ARS, USDA, POB 1848, University, MS 38677 USA. EM agnes.rimando@ars.usda.gov NR 38 TC 1 Z9 1 U1 5 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD MAR 30 PY 2016 VL 96 IS 5 BP 1666 EP 1671 DI 10.1002/jsfa.7269 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DG8EX UT WOS:000372316700032 PM 25996649 ER PT J AU Fagerquist, CK Zaragoza, WJ AF Fagerquist, Clifton K. Zaragoza, William J. TI Bacteriophage cell lysis of Shiga toxin-producing Escherichia coli for top-down proteomic identification of Shiga toxins 1 & 2 using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID DESORPTION IONIZATION-TIME; RAPID IDENTIFICATION; BACTERIAL IDENTIFICATION; INTACT MICROORGANISMS; PROTEIN BIOMARKERS; SEQUENCE; MALDI; GENE; MS; SUBTYPES AB RationalAnalysis of bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) often relies upon sample preparation methods that result in cell lysis, e.g. bead-beating. However, Shiga toxin-producing Escherichia coli (STEC) can undergo bacteriophage-induced cell lysis triggered by antibiotic exposure that may allow greater selectivity of the proteins extracted. MethodsWe have developed a sample preparation method for selective extraction of bacteriophage-encoded proteins and specifically Shiga toxins 1 and 2 (Stx1 & 2) expressed from STEC strains induced by DNA-damaging antibiotics. STEC strains were cultured overnight on agar supplemented with ciprofloxacin, mitomycin-C or an iron chelator to induce the bacteriophage lytic cycle with concomitant expression and release of Stx1 and/or Stx2. Sample preparation relied exclusively on bacteriophage lysis for release Stx into the extraction solution. ResultsThree clinical STEC strains were analyzed by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and top-down proteomics analysis: E. coli O157:H7 strain EDL933, E. coli O91:H21 strain B2F1 and E. coli O26:H11 strain ECRC #05.2217. The B-subunit of Stx1a of EDL933 was detected and identified even though it was similar to 100-fold less abundant than the B-subunit of Stx2a that had been identified previously for this strain. Two bacteriophage-encoded proteins were also identified: L0117 and L0136. The B-subunits of Stx2d of strain B2F1 and Stx1a of strain ECRC #05.2217 were also detected and identified. ConclusionsBacteriophage lysis appeared to enhance the detection sensitivity of Stx for these STEC strains compared to previous work using mechanical lysis. Detection/identification of other bacteriophage-encoded proteins (beyond Stx) tends to support the hypothesis of Stx release by bacteriophage cell lysis. Published in 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Fagerquist, Clifton K.; Zaragoza, William J.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. RP Fagerquist, CK (reprint author), ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM clifton.fagerquist@ars.usda.gov FU USDA-ARS CRIS [5325-42000-047-00D] FX This research was supported by USDA-ARS CRIS Project No. 5325-42000-047-00D. NR 45 TC 2 Z9 2 U1 6 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0951-4198 EI 1097-0231 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PD MAR 30 PY 2016 VL 30 IS 6 BP 671 EP 680 DI 10.1002/rcm.7507 PG 10 WC Biochemical Research Methods; Chemistry, Analytical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA DD8MT UT WOS:000370181700001 PM 26864518 ER PT J AU Song, HB Yang, CH Zhang, J Hoffmann, WC He, DJ Thomasson, JA AF Song, Huaibo Yang, Chenghai Zhang, Jian Hoffmann, Wesley Clint He, Dongjian Thomasson, J. Alex TI Comparison of mosaicking techniques for airborne images from consumer-grade cameras SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE accuracy assessment; aerial applicator; airborne imagery; image mosaicking; mosaicking software ID DIGITAL CAMERAS; UAV AB Images captured from airborne imaging systems can be mosaicked for diverse remote sensing applications. The objective of this study was to identify appropriate mosaicking techniques and software to generate mosaicked images for use by aerial applicators and other users. Three software packages-Photoshop CC, Autostitch, and Pix4Dmapper-were selected for mosaicking airborne images acquired from a large cropping area. Ground control points were collected for georeferencing the mosaicked images and for evaluating the accuracy of eight mosaicking techniques. Analysis and accuracy assessment showed that Pix4Dmapper can be the first choice if georeferenced imagery with high accuracy is required. The spherical method in Photoshop CC can be an alternative for cost considerations, and Autostitch can be used to quickly mosaic images with reduced spatial resolution. The results also showed that the accuracy of image mosaicking techniques could be greatly affected by the size of the imaging area or the number of the images and that the accuracy would be higher for a small area than for a large area. The results from this study will provide useful information for the selection of image mosaicking software and techniques for aerial applicators and other users. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. C1 [Song, Huaibo; He, Dongjian] Northwest A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China. [Song, Huaibo; Yang, Chenghai; Zhang, Jian; Hoffmann, Wesley Clint] ARS, USDA, Southern Plains Agr Res Ctr, 3103 F&B Rd, College Stn, TX 77845 USA. [Song, Huaibo; Thomasson, J. Alex] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Zhang, Jian] Huazhong Agr Univ, Coll Resource & Environm, 1 Shizishan St, Wuhan 430070, Hubei, Peoples R China. RP Yang, CH (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, 3103 F&B Rd, College Stn, TX 77845 USA. EM chenghai.yang@ars.usda.gov FU China Scholarship Council FX This project was conducted as part of a visiting scholar research program, and the first and third authors were financially supported by the China Scholarship Council. The authors wish to thank Fred Gomez and Lee Denham of USDA-ARS in College Station, Texas, for acquiring the images and their assistance in collecting the GCPs for this study. The authors also wish to thank the developers of all the software and the authors of the papers we cited in this research for their excellent work. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 0 Z9 0 U1 10 U2 14 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD MAR 29 PY 2016 VL 10 AR 016030 DI 10.1117/1.JRS.10.016030 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DJ4OJ UT WOS:000374185500002 ER PT J AU Beckley, CS Shaban, S Palmer, GH Hudak, AT Noh, SM Futse, JE AF Beckley, Carl S. Shaban, Salisu Palmer, Guy H. Hudak, Andrew T. Noh, Susan M. Futse, James E. TI Disaggregating Tropical Disease Prevalence by Climatic and Vegetative Zones within Tropical West Africa SO PLOS ONE LA English DT Article ID INFECTIOUS-DISEASES; DOMESTIC RUMINANTS; BORNE DISEASES; ANIMAL HEALTH; TICKS; VECTOR; SURVEILLANCE; DATABASE; GHANA AB Tropical infectious disease prevalence is dependent on many socio-cultural determinants. However, rainfall and temperature frequently underlie overall prevalence, particularly for vector-borne diseases. As a result these diseases have increased prevalence in tropical as compared to temperate regions. Specific to tropical Africa, the tendency to incorrectly infer that tropical diseases are uniformly prevalent has been partially overcome with solid epidemiologic data. This finer resolution data is important in multiple contexts, including understanding risk, predictive value in disease diagnosis, and population immunity. We hypothesized that within the context of a tropical climate, vector-borne pathogen prevalence would significantly differ according to zonal differences in rainfall, temperature, relative humidity and vegetation condition. We then determined if these environmental data were predictive of pathogen prevalence. First we determined the prevalence of three major pathogens of cattle, Anaplasma marginale, Babesia bigemina and Theileria spp, in the three vegetation zones where cattle are predominantly raised in Ghana: Guinea savannah, semi-deciduous forest, and coastal savannah. The prevalence of A. marginale was 63%, 26% for Theileria spp and 2% for B. bigemina. A. marginale and Theileria spp. were significantly more prevalent in the coastal savannah as compared to either the Guinea savanna or the semi-deciduous forest, supporting acceptance of the first hypothesis. To test the predictive power of environmental variables, the data over a three year period were considered in best subsets multiple linear regression models predicting prevalence of each pathogen. Corrected Akaike Information Criteria (AICc) were assigned to the alternative models to compare their utility. Competitive models for each response were averaged using AICc weights. Rainfall was most predictive of pathogen prevalence, and EVI also contributed to A. marginale and B. bigemina prevalence. These findings support the utility of environmental data for understanding vector-borne disease epidemiology on a regional level within a tropical environment. C1 [Shaban, Salisu; Futse, James E.] Univ Ghana, Dept Anim Sci, Coll Basic & Appl Sci, Legon, Ghana. [Beckley, Carl S.] CSIR, Anim Res Inst, Accra, Ghana. [Noh, Susan M.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Palmer, Guy H.; Noh, Susan M.] Washington State Univ, Dept Vet Microbiol & Pathol, Program Vector Borne Dis, Pullman, WA 99164 USA. [Palmer, Guy H.; Noh, Susan M.; Futse, James E.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Hudak, Andrew T.] US Forest Serv, Rocky Mt Res Stn, USDA, Moscow, ID USA. RP Futse, JE (reprint author), Univ Ghana, Dept Anim Sci, Coll Basic & Appl Sci, Legon, Ghana.; Futse, JE (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM jefutse@ug.edu.gh FU Wellcome Trust [097171/Z/11/Z]; International Foundation for Science [B/5120-1]; National Institute of Health (NIH) [R37 AI44005]; United States Department of Agriculture -Agricultural Research Service [USDA-ARS 5348-32000-033-00D]; Wellcome trust; NIH [R37 AI44005]; USDA-ARS [5348-32000-033-00D]; Jan and Jack Creighton Endowment fund FX This work was supported by The Wellcome Trust for the fellowship in Public Health and Tropical Medicine grant, #097171/Z/11/Z, URL: www.wellcome.ac.uk/.../Publichealth-and-tropical-medicine/wtd025883.htm, to JEF; International Foundation for Science, #B/5120-1, URL: http://www.ifs.se/to JEF; National Institute of Health (NIH) Grant R37 AI44005, URL: http://nih.gov/ to GHP; and United States Department of Agriculture -Agricultural Research Service, (USDA-ARS 5348-32000-033-00D, URL: http://www.usda.gov/wps/portal/usda/usdahome?, to GHP. The Wellcome trust provided funding for the study, including money for reagents, data collection, and preparation of the manuscript. NIH and USDA-ARS provided some molecular biology reagents. Jan and Jack Creighton Endowment fund provided travel support for Professor Palmer to visit project site in Ghana.; This work was supported by the International Foundation for Science grant B/5120-1, the Wellcome Trust for the fellowship in Public Health and Tropical Medicine grant 097171/Z/11/Z, NIH Grant (R37 AI44005), USDA-ARS 5348-32000-033-00D, and the Jan and Jack Creighton Endowment fund. We wish to thank Crystal Davis and Alexis Gantz for graphics support and the Ghana Meteorological Agency for providing the rainfall, temperature, and relative humidity data for the study period. NR 32 TC 1 Z9 1 U1 3 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 29 PY 2016 VL 11 IS 3 AR e0152560 DI 10.1371/journal.pone.0152560 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH9KB UT WOS:000373113900076 PM 27022740 ER PT J AU Tang, W Zheng, Y Dong, J Yu, J Yue, JY Liu, FF Guo, XH Huang, SX Wisniewski, M Sun, JQ Niu, XL Ding, J Liu, J Fei, ZJ Liu, YS AF Tang, Wei Zheng, Yi Dong, Jing Yu, Jia Yue, Junyang Liu, Fangfang Guo, Xiuhong Huang, Shengxiong Wisniewski, Michael Sun, Jiaqi Niu, Xiangli Ding, Jian Liu, Jia Fei, Zhangjun Liu, Yongsheng TI Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis) SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE fruit development and ripening; long noncoding RNAs; alternative splicing; novel genes; transcriptome profiling; gene expression ID SUCROSE-PHOSPHATE SYNTHASE; GENOME-WIDE ANALYSIS; ANTHOCYANIN BIOSYNTHESIS; ARABIDOPSIS-THALIANA; METABOLIZING ENZYMES; GENE; TRANSPORTERS; PLANTS; LANDSCAPE; DELICIOSA AB Genomic and transcriptomic data on kiwifruit (Actinidia chinensis) in public databases are very limited despite its nutritional and economic value. Previously, we have constructed and sequenced nine fruit RNA-Seq libraries of A. chinensis "Hongyang" at immature, mature, and postharvest ripening stages of fruit development, and generated over 66.2 million paired-end and 24.4 million single-end reads. From this dataset, here we have identified 7051 long noncoding RNAs (IncRNAs), 29,327 alternative splicing (AS) events and 2980 novel protein-coding genes that were not annotated in the draft genome of "Hongyang." AS events were demonstrated in genes involved in the synthesis of nutritional metabolites in fruit, such as ascorbic acids, carotenoids, anthocyanins, and chlorophylls, and also in genes in the ethylene signaling pathway, which plays an indispensable role in fruit ripening. Additionally, transcriptome profiles and the contents of sugars, organic and main amino acids were compared between immature, mature, and postharvest ripening stages in kiwifruits. A total of 5931 differentially expressed genes were identified, including those associated with the metabolism of sugar, organic acid, and main amino acids. The data generated in this study provide a foundation for further studies of fruit development and ripening in kiwifruit, and identify candidate genes and regulatory elements that could serve as targets for improving important agronomic traits through marker assisted breeding and biotechnology. C1 [Tang, Wei; Yue, Junyang; Huang, Shengxiong; Niu, Xiangli; Liu, Jia; Liu, Yongsheng] Hefei Univ Technol, Sch Biotechnol & Food Engn, Dept Biol Sci, Hefei, Peoples R China. [Tang, Wei; Dong, Jing; Yu, Jia; Liu, Fangfang; Guo, Xiuhong; Sun, Jiaqi; Liu, Yongsheng] Sichuan Univ, Coll Life Sci, State Key Lab Hydraul & Mt River Engn, Minist Educ,Key Lab Bioresource, Chengdu 610064, Peoples R China. [Zheng, Yi; Fei, Zhangjun] Cornell Univ, Plant Biol Sect, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Wisniewski, Michael] USDA ARS, Kearneysville, WV USA. [Ding, Jian] Sichuan Tech Exchange Ctr, Chengdu, Peoples R China. RP Liu, J; Liu, YS (reprint author), Hefei Univ Technol, Sch Biotechnol & Food Engn, Dept Biol Sci, Hefei, Peoples R China.; Liu, YS (reprint author), Sichuan Univ, Coll Life Sci, State Key Lab Hydraul & Mt River Engn, Minist Educ,Key Lab Bioresource, Chengdu 610064, Peoples R China. EM jialiu1983@163.com; liuyongsheng1122@hfut.edu.cn RI Zheng, Yi/F-6150-2016 OI Zheng, Yi/0000-0002-8042-7770 FU National Natural Science Foundation of China [31471157, 31461143008]; National Basic Research Program of China (973 Program) [2011CB100401]; Key Project from Government of Sichuan Province [2013NZ0014]; Provincial Natural Science Research Project of Anhui Colleges [KJ2014ZD25]; HFUT; USDA [58-1931-4-008FN]; National Science Fund for Distinguished Young Scholars [308250] FX This work was supported by National Natural Science Foundation of China (31471157 and 31461143008), a National Basic Research Program of China (973 Program) (2011CB100401), a Key Project from Government of Sichuan Province (2013NZ0014), a Provincial Natural Science Research Project of Anhui Colleges (KJ2014ZD25), a Project between HFUT and USDA (58-1931-4-008FN), and a National Science Fund for Distinguished Young Scholars (308250). NR 77 TC 0 Z9 0 U1 16 U2 35 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 29 PY 2016 VL 7 AR 335 DI 10.3389/fpls.2016.00335 PG 15 WC Plant Sciences SC Plant Sciences GA DH5ZT UT WOS:000372871500001 PM 27594858 ER PT J AU Manter, DK Korsa, M Tebbe, C Delgado, JA AF Manter, Daniel K. Korsa, Matthew Tebbe, Caleb Delgado, Jorge A. TI myPhyloDB: a local web server for the storage and analysis of metagenomic data SO DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION LA English DT Article ID SEQUENCING DATA; BETA DIVERSITY; COMMUNITIES AB myPhyloDB v.1.1.2 is a user-friendly personal database with a browser-interface designed to facilitate the storage, processing, analysis, and distribution of microbial community populations (e.g. 16S metagenomics data). MyPhyloDB archives raw sequencing files, and allows for easy selection of project(s)/sample(s) of any combination from all available data in the database. The data processing capabilities of myPhyloDB are also flexible enough to allow the upload and storage of pre-processed data, or use the built-in Mothur pipeline to automate the processing of raw sequencing data. myPhyloDB provides several analytical (e.g. analysis of covariance, t-tests, linear regression, differential abundance (DESeq2), and principal coordinates analysis (PCoA)) and normalization (rarefaction, DESeq2, and proportion) tools for the comparative analysis of taxonomic abundance, species richness and species diversity for projects of various types (e.g. human-associated, human gut microbiome, air, soil, and water) for any taxonomic level(s) desired. Finally, since myPhyloDB is a local web-server, users can quickly distribute data between colleagues and end-users by simply granting others access to their personal myPhyloDB database. myPhyloDB is available at http://www.ars.usda.gov/services/software/download.htm?softwareid-472 and more information along with tutorials can be found on our website http://www.myphylodb.org. C1 [Manter, Daniel K.; Korsa, Matthew; Tebbe, Caleb; Delgado, Jorge A.] USDA ARS, Soil Plant Nutrient Res, Ft Collins, CO 80526 USA. RP Manter, DK (reprint author), USDA ARS, Soil Plant Nutrient Res, Ft Collins, CO 80526 USA. EM Daniel.manter@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1758-0463 J9 DATABASE-OXFORD JI Database PD MAR 28 PY 2016 AR baw039 DI 10.1093/database/baw037 PG 9 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA DJ3GW UT WOS:000374093800001 ER PT J AU Fowler, DB N'Diaye, A Laudencia-Chingcuanco, D Pozniak, CJ AF Fowler, D. B. N'Diaye, A. Laudencia-Chingcuanco, D. Pozniak, C. J. TI Quantitative Trait Loci Associated with Phenological Development, Low-Temperature Tolerance, Grain Quality, and Agronomic Characters in Wheat (Triticum aestivum L.) SO PLOS ONE LA English DT Article ID DOUBLED HAPLOID POPULATION; CONSENSUS GENETIC MAPS; WINTER-WHEAT; BREAD-WHEAT; FROST-TOLERANCE; EM THELL; IDENTIFICATION; VERNALIZATION; CEREALS; YIELD AB Plants must respond to environmental cues and schedule their development in order to react to periods of abiotic stress and commit fully to growth and reproduction under favorable conditions. This study was initiated to identify SNP markers for characters expressed from the seedling stage to plant maturity in spring and winter wheat (Triticum aestivum L.) genotypes adapted to western Canada. Three doubled haploid populations with the winter cultivar 'Norstar' as a common parent were developed and genotyped with a 90K Illumina iSelect SNP assay and a 2,998.9 cM consensus map with 17,541 markers constructed. High heritability's reflected large differences among the parents and relatively low genotype by environment interactions for all characters considered. Significant QTL were detected for the 15 traits examined. However, different QTL for days to heading in controlled environments and the field provided a strong reminder that growth and development are being orchestrated by environmental cues and caution should be exercised when extrapolating conclusions from different experiments. A QTL on chromosome 6A for minimum final leaf number, which determines the rate of phenological development in the seedling stage, was closely linked to QTL for low-temperature tolerance, grain quality, and agronomic characters expressed up to the time of maturity. This suggests phenological development plays a critical role in programming subsequent outcomes for many traits. Transgressive segregation was observed for the lines in each population and QTL with additive effects were identified suggesting that genes for desirable traits could be stacked using Marker Assisted Selection. QTL were identified for characters that could be transferred between the largely isolated western Canadian spring and winter wheat gene pools demonstrating the opportunities offered by Marker Assisted Selection to act as bridges in the identification and transfer of useful genes among related genetic islands while minimizing the drag created by less desirable genes. C1 [Fowler, D. B.; N'Diaye, A.; Pozniak, C. J.] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK S7N 5A8, Canada. [Fowler, D. B.; N'Diaye, A.; Pozniak, C. J.] Univ Saskatchewan, Ctr Crop Dev, Saskatoon, SK S7N 5A8, Canada. [Laudencia-Chingcuanco, D.] USDA ARS WRRC, Crop Improvement & Genet Res Unit, 800 Buchanan St, Albany, CA 94710 USA. RP Fowler, DB (reprint author), Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK S7N 5A8, Canada.; Fowler, DB (reprint author), Univ Saskatchewan, Ctr Crop Dev, Saskatoon, SK S7N 5A8, Canada. EM Brian.Fowler@usask.ca FU Ducks Unlimited Canada; Saskatchewan Agriculture and Food; Genome Canada FX Ducks Unlimited Canada, Saskatchewan Agriculture and Food, and Genome Canada provided long-term financial support for this project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 62 TC 0 Z9 0 U1 11 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 28 PY 2016 VL 11 IS 3 AR e0152185 DI 10.1371/journal.pone.0152185 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH5EH UT WOS:000372807800031 PM 27019468 ER PT J AU Wang, X Liu, ZL Weber, SA Zhang, XP AF Wang, Xu Liu, Z. Lewis Weber, Scott A. Zhang, Xiaoping TI Two New Native beta-Glucosidases from Clavispora NRRL Y-50464 Confer Its Dual Function as Cellobiose Fermenting Ethanologenic Yeast SO PLOS ONE LA English DT Article ID NEUROSPORA-CRASSA; FAMILY 3; SUBSTRATE-SPECIFICITY; BIOETHANOL PRODUCTION; QRT-PCR; FERMENTATION; PURIFICATION; HYDROLASE; CELLULOSE; BIOMASS AB Yeast strain Clavispora NRRL Y-50464 is able to produce cellulosic ethanol from lignocellulosic materials without addition of external beta-glucosidase by simultaneous saccharification and fermentation. A beta-glucosidase BGL1 protein from this strain was recently reported supporting its cellobiose utilization capability. Here, we report two additional new beta-glucosidase genes encoding enzymes designated as BGL2 and BGL3 from strain NRRL Y-50464. Quantitative gene expression was analyzed and the gene function of BGL2 and BGL3 was confirmed by heterologous expression using cellobiose as a sole carbon source. Each gene was cloned and partially purified protein obtained separately for direct enzyme assay using varied substrates. Both proteins showed the highest specific activity at pH 5 and relatively strong affinity with a K-m of 0.08 and 0.18 mM for BGL2 and BGL3, respectively. The optimum temperature was found to be 50 degrees C for BGL2 and 55 degrees C for BGL3. Both proteins were able to hydrolyze 1,4 oligosaccharides evaluated in this study. They also showed a strong resistance to glucose product inhibition with a K-i of 61.97 and 38.33 mM for BGL2 and BGL3, respectively. While BGL3 was sensitive showing a significantly reduced activity to 4% ethanol, BGL2 demonstrated tolerance to ethanol. Its activity was enhanced in the presence of ethanol but reduced at concentrations greater than 16%. The presence of the fermentation inhibitors furfural and HMF did not affect the enzyme activity. Our results suggest that a beta-glucosidase gene family exists in Clavispora NRRL Y-50464 with at least three members in this group that validate its cellobiose hydrolysis functions for lower-cost cellulosic ethanol production. Results of this study confirmed the cellobiose hydrolysis function of strain NRRL Y-50464, and further supported this dual functional yeast as a candidate for lower-cost cellulosic ethanol production and next-generation biocatalyst development in potential industrial applications. C1 [Wang, Xu; Zhang, Xiaoping] Sichuan Agr Univ, Chengdu, Peoples R China. [Wang, Xu; Liu, Z. Lewis; Weber, Scott A.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Liu, ZL (reprint author), ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM ZLewis.Liu@ars.usda.gov NR 47 TC 0 Z9 0 U1 2 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 24 PY 2016 VL 11 IS 3 AR e0151293 DI 10.1371/journal.pone.0151293 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH3SV UT WOS:000372708000026 PM 27011316 ER PT J AU Sei, JJ Waters, RA Kenney, M Barlow, JW Golde, WT AF Sei, Janet J. Waters, Ryan A. Kenney, Mary Barlow, John W. Golde, William T. TI Effect of Foot-and-Mouth Disease Virus Infection on the Frequency, Phenotype and Function of Circulating Dendritic Cells in Cattle SO PLOS ONE LA English DT Article ID RESPIRATORY SYNDROME VIRUS; ANTIBODY-RESPONSE; KAPPA-B; BLOOD; SWINE; ALPHA; INTERFERON; EXPRESSION; COMPLEXES; LYMPHOPENIA AB Foot-and-mouth disease virus (FMDV) is a highly contagious virus that causes one of the most devastating diseases in cloven-hoofed animals. Disease symptoms develop within 2 to 3 days of exposure and include fever and vesicular lesions on the tongue and hooves. Dendritic cells (DC) play an essential role in protective immune responses against pathogens. Therefore, investigating their role during FMDV infection would lead to a better understanding of host-pathogen interactions. In this study, following infection of cattle with FMDV, we investigated the frequency and function of conventional (cDC) and plasmacytoid DC (pDC) in blood by using multi-color flow cytometry. We show that the frequency of cDC and pDC increased following FMDV infection and peaked 3 to 4 days post-infection. During peak viremia, the cattle became lymphopenic, the expression of MHC class II molecules on cDC and pDC was dramatically down-regulated, the processing of exogenous antigen by cDC and pDC was impaired, and there was an increase in IL-10 production by DC and monocytes. Notably, after clearance of FMDV from the blood, MHC class II expression returned to pre-infection levels. Altogether, our study demonstrates that in cattle, FMDV inhibits the function of DC, thereby retarding the initiation of adaptive immune responses, potentially enhancing virus shedding during the acute phase of infection. C1 [Sei, Janet J.; Waters, Ryan A.; Kenney, Mary; Golde, William T.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Sei, Janet J.; Barlow, John W.] Univ Vermont, Dept Anim & Vet Sci, Burlington, VT USA. [Sei, Janet J.] Merck & Co Inc, Kenilworth, NJ USA. [Waters, Ryan A.] Pirbright Inst, Pirbright, England. RP Golde, WT (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. EM William.Golde@ARS.USDA.GOV FU US Department of Agriculture, Agricultural Research Service [CRIS 1940-32000-057-00D]; US Department of Homeland Security, Science and Technology Directorate IAA [HQPM-13-X-00110]; National Science Foundation [0965346] FX This work was supported by the US Department of Agriculture, Agricultural Research Service, (http://www.ars.usda.gov/) CRIS 1940-32000-057-00D (W.T.G.); US Department of Homeland Security, Science and Technology Directorate (http://www.dhs.gov/science-and-technology) IAA #HQPM-13-X-00110 (W.T.G.); and National Science Foundation (http://www.nsf.gov/bio/bread) grant #0965346, under the BREAD program (J.W.B. and W.T.G.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 46 TC 0 Z9 0 U1 3 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 23 PY 2016 VL 11 IS 3 AR e0152192 DI 10.1371/journal.pone.0152192 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH3QJ UT WOS:000372701200108 PM 27008425 ER PT J AU Albright, VC Hellmich, RL Coats, JR AF Albright, Vurtice C., III Hellmich, Richard L. Coats, Joel R. TI A Review of Cry Protein Detection with Enzyme-Linked Immunosorbent Assays SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Review DE bioassays; cross validation; Bt proteins; Cry protein; ELISA; environmental fate; transgenic crops ID THURINGIENSIS SUBSP KURSTAKI; GENETICALLY-MODIFIED MAIZE; MONARCH BUTTERFLY LARVAE; TRANSGENIC TOMATO PLANTS; BT CORN POLLEN; BACILLUS-THURINGIENSIS; CRY3BB1 PROTEIN; ENVIRONMENTAL FATE; DELTA-ENDOTOXIN; RISK-ASSESSMENT AB The widespread use of Cry proteins in insecticide formulations and transgenic crops for insect control has led to an increased interest in the environmental fate of these proteins. Although several detection methods are available to monitor the fate of Cry proteins in the environment, enzyme-linked irnmunosorbent assays (ELISAs) have emerged as the preferred detection method, due to their cost-effectiveness, ease of use, and rapid results. Validation of ELISAs is necessary to ensure accurate measurements of Cry protein concentrations in the environment. Validation methodology has 'been extensively researched and published for the areas of sensitivity, specificity, accuracy, and precision; however, cross validation of ELISA results has been studied to a lesser extent. This review discusses the use of ELISAs for detection of Cry proteins in environmental samples and validation of ELISAs and introduces cross validation. The state of Cry protein environmental fate research is considered through a critical review of published literature to identify areas where the use.of validation protocols can be improved. C1 [Albright, Vurtice C., III; Coats, Joel R.] Iowa State Univ, Dept Entomol, Pesticide Toxicol Lab, 110 Insectary, Ames, IA 50011 USA. [Hellmich, Richard L.] ARS, Corn Insects & Crop Genet Res Unit, USDA, Genet Lab 110, Ames, IA 50011 USA. RP Coats, JR (reprint author), Iowa State Univ, Dept Entomol, Pesticide Toxicol Lab, 110 Insectary, Ames, IA 50011 USA. EM jcoats@iastate.edu NR 129 TC 1 Z9 1 U1 10 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 23 PY 2016 VL 64 IS 11 BP 2175 EP 2189 DI 10.1021/acs.jafc.5b03766 PG 15 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DH5TH UT WOS:000372854300001 PM 26949828 ER PT J AU Walker, WB Gonzalez, F Garczynski, SF Witzgall, P AF Walker, William B., III Gonzalez, Francisco Garczynski, Stephen F. Witzgall, Peter TI The chemosensory receptors of codling moth Cydia pomonella-expression in larvae and adults SO SCIENTIFIC REPORTS LA English DT Article ID IONOTROPIC GLUTAMATE RECEPTORS; RNA-SEQ DATA; BOMBYX-MORI; GUSTATORY RECEPTOR; ANTENNAL RESPONSE; ODORANT RECEPTOR; REFERENCE GENOME; MOLECULAR-BASIS; DROSOPHILA; SEQUENCE AB Olfaction and gustation play critical roles in the life history of insects, mediating vital behaviors such as food, mate and host seeking. Chemosensory receptor proteins, including odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) function to interface the insect with its chemical environment. Codling moth, Cydia pomonella, is a worldwide pest of apple, pear and walnut, and behavior-modifying semiochemicals are used for environmentally safe control. We produced an Illumina-based transcriptome from antennae of males and females as well as neonate head tissue, affording a qualitative and quantitative analysis of the codling moth chemosensory receptor repertoire. We identified 58 ORs, 20 GRs and 21 IRs, and provide a revised nomenclature that is consistent with homologous sequences in related species. Importantly, we have identified several OR transcripts displaying sex-biased expression in adults, as well as larval-enriched transcripts. Our analyses have expanded annotations of the chemosensory receptor gene families, and provide first-time transcript abundance estimates for codling moth. The results presented here provide a strong foundation for future work on codling moth behavioral physiology and ecology at the molecular level, and may lead to the development of more precise biorational control strategies. C1 [Walker, William B., III] Max Planck Inst Chem Ecol, Dept Evolutionary Neuroethol, D-07745 Jena, Germany. [Walker, William B., III; Gonzalez, Francisco; Witzgall, Peter] Swedish Univ Agr Sci, Dept Plant Protect Biol, Chem Ecol Unit, S-23053 Alnarp, Sweden. [Garczynski, Stephen F.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. RP Walker, WB (reprint author), Max Planck Inst Chem Ecol, Dept Evolutionary Neuroethol, D-07745 Jena, Germany.; Walker, WB (reprint author), Swedish Univ Agr Sci, Dept Plant Protect Biol, Chem Ecol Unit, S-23053 Alnarp, Sweden. EM william.b.walker.iii@slu.se FU Linnaeus initiative "Insect Chemical Ecology, Ethology and Evolution (IC-E3)" (Formas, SLU) [2011 1370]; Carl Tryggers Stiftelse for Vetenskaplig Forskning; SLU faculty of Landscape Architecture, Horticulture and Crop Production Sciences) FX We thank Maria Sousa for assistance with insect rearing and tissue dissections; Drs Sandeep Kumar Kushwaha and Amit Roy for assistance with bioinformatics scripts and processing; and Dr. Emmanuelle Jacquin-Joly for assistance in updating previously annotated Genbank deposits. We thank Dr. Jonas Bengtsson for helpful discussions on the data, Prof. Richard D. Newcomb, for early access to previously unpublished OR sequences, and also Drs. Joe Hull and Kylie Swisher for critical review of an earlier version of this manuscript. The authors acknowledge support from Science for Life Laboratory, the National Genomics Infrastructure (NGI), and Uppmax for providing assistance in massive parallel sequencing and computational infrastructure. Financial support for this study comes from the Linnaeus initiative "Insect Chemical Ecology, Ethology and Evolution (IC-E3)" (Formas, SLU; Formas Dnr 2011 1370, Carl Tryggers Stiftelse for Vetenskaplig Forskning and the SLU faculty of Landscape Architecture, Horticulture and Crop Production Sciences). NR 60 TC 2 Z9 2 U1 4 U2 31 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 23 PY 2016 VL 6 AR 23518 DI 10.1038/srep23518 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH2IH UT WOS:000372607900001 PM 27006164 ER PT J AU Xu, LY Hou, YL Bickhart, DM Zhou, Y Hay, EA Song, JZ Sonstegard, TS Van Tassell, CP Liu, GE AF Xu, Lingyang Hou, Yali Bickhart, Derek M. Zhou, Yang Hay, El Hamidi Abdel Song, Jiuzhou Sonstegard, Tad S. Van Tassell, Curtis P. Liu, George E. TI Population-genetic properties of differentiated copy number variations in cattle SO SCIENTIFIC REPORTS LA English DT Article ID MULTILOCUS GENOTYPE DATA; POTENTIAL INVOLVEMENT; STRUCTURAL VARIATION; HUMAN ADAPTATION; HUMAN GENOME; DIVERSITY; EVOLUTION; VARIANTS; TRAITS; POLYMORPHISMS AB While single nucleotide polymorphism (SNP) is typically the variant of choice for population genetics, copy number variation (CNV) which comprises insertion, deletion and duplication of genomic sequence, is an informative type of genetic variation. CNVs have been shown to be both common in mammals and important for understanding the relationship between genotype and phenotype. However, CNV differentiation, selection and its population genetic properties are not well understood across diverse populations. We performed a population genetics survey based on CNVs derived from the BovineHD SNP array data of eight distinct cattle breeds. We generated high resolution results that show geographical patterns of variations and genome-wide admixture proportions within and among breeds. Similar to the previous SNP-based studies, our CNV-based results displayed a strong correlation of population structure and geographical location. By conducting three pairwise comparisons among European taurine, African taurine, and indicine groups, we further identified 78 unique CNV regions that were highly differentiated, some of which might be due to selection. These CNV regions overlapped with genes involved in traits related to parasite resistance, immunity response, body size, fertility, and milk production. Our results characterize CNV diversity among cattle populations and provide a list of lineage-differentiated CNVs. C1 [Xu, Lingyang; Bickhart, Derek M.; Zhou, Yang; Hay, El Hamidi Abdel; Sonstegard, Tad S.; Van Tassell, Curtis P.; Liu, George E.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Xu, Lingyang; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Hou, Yali] Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China. [Zhou, Yang] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China. [Sonstegard, Tad S.] Recombinetics Inc, St Paul, MN 55104 USA. RP Liu, GE (reprint author), ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. EM George.Liu@ars.usda.gov OI Bickhart, Derek/0000-0003-2223-9285 FU USDA NIFA [2011-67015-30183]; Youth Innovation Promotion Association, Chinese Academy of Sciences FX We thank members of the Bovine HapMap Consortium for sharing their data. We thank Reuben Anderson and Alexandre Dimitriv for technical assistance. This work was supported in part by AFRI grant No. 2011-67015-30183 from USDA NIFA (G.E.L.) and Youth Innovation Promotion Association, Chinese Academy of Sciences (Y.H.). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 60 TC 4 Z9 4 U1 4 U2 8 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 23 PY 2016 VL 6 AR 23161 DI 10.1038/srep23161 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH2IV UT WOS:000372609400001 PM 27005566 ER PT J AU Zhang, ML Zhao, Y Harrington, PD Chen, P AF Zhang, Mengliang Zhao, Yang Harrington, Peter de B. Chen, Pei TI Differentiation of Aurantii fructus immaturus and Fructus poniciri trifoliatae immaturus by Flow-Injection with Ultraviolet Spectroscopic Detection and Proton Nuclear Magnetic Resonance Using Partial Least-Squares Discriminant Analysis SO ANALYTICAL LETTERS LA English DT Article DE Fructus poniciri trifoliatae immaturus; proton nuclear magnetic resonance; partial least-squares discriminant analysis; Aurantii fructus immaturus; flow-injection with ultraviolet spectroscopic detection; nutraceutical ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SPECTROMETRY; CONSTITUENTS; SAMPLES AB Two simple fingerprinting methods, flow-injection coupled to ultraviolet spectroscopy and proton nuclear magnetic resonance, were used for discriminating between Aurantii fructus immaturus and Fructus poniciri trifoliatae immaturus. Both methods were combined with partial least-squares discriminant analysis. In the flow-injection method, four data representations were evaluated: total ultraviolet absorbance chromatograms, averaged ultraviolet spectra, absorbance at 193, 205, 225, and 283nm, and absorbance at 225 and 283nm. Prediction rates of 100% were achieved for all data representations by partial least-squares discriminant analysis using leave-one-sample-out cross-validation. The prediction rate for the proton nuclear magnetic resonance data by partial least-squares discriminant analysis with leave-one-sample-out cross-validation was also 100%. A new validation set of data was collected by flow-injection with ultraviolet spectroscopic detection two weeks later and predicted by partial least-squares discriminant analysis models constructed by the initial data representations with no parameter changes. The classification rates were 95% with the total ultraviolet absorbance chromatograms datasets and 100% with the other three datasets. Flow-injection with ultraviolet detection and proton nuclear magnetic resonance are simple, high throughput, and low-cost methods for discrimination studies. C1 [Zhang, Mengliang; Zhao, Yang; Chen, Pei] USDA ARS, Food Composit & Methods Dev Lab, BHNRC, 10300 Baltimore Ave,Bldg161,BARC E, Beltsville, MD 20705 USA. [Zhang, Mengliang; Harrington, Peter de B.] Ohio Univ, Dept Chem & Biochem, Clippinger Lab, Ctr Intelligent Chem Instrumentat, Athens, OH 45701 USA. RP Chen, P (reprint author), USDA ARS, Food Composit & Method Dev Lab, BHNRC, 10300 Baltimore Ave,Bldg161,BARC E, Beltsville, MD 20705 USA. EM pei.chen@ars.usda.gov RI Zhang, Mengliang/J-3573-2015 OI Zhang, Mengliang/0000-0001-8366-0052 FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements of the National Institutes of Health of the U.S. Health and Human Services FX This research was supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health of the U.S. Health and Human Services. NR 20 TC 0 Z9 0 U1 5 U2 13 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0003-2719 EI 1532-236X J9 ANAL LETT JI Anal. Lett. PD MAR 23 PY 2016 VL 49 IS 5 SI SI BP 711 EP 722 DI 10.1080/00032719.2015.1045588 PG 12 WC Chemistry, Analytical SC Chemistry GA DF9YK UT WOS:000371718400010 PM 27013744 ER PT J AU Laclaustra, M Casasnovas, JA Fernandez-Ortiz, A Fuster, V Leon-Latre, M Jimenez-Borreguero, LJ Pocovi, M Hurtado-Roca, Y Ordovas, JM Jarauta, E Guallar, E Ibanez, B Civeira, F AF Laclaustra, Martin Casasnovas, Jose A. Fernandez-Ortiz, Antonio Fuster, Valentin Leon-Latre, Monserrat Jimenez-Borreguero, Luis J. Pocovi, Miguel Hurtado-Roca, Yamilee Ordovas, Jose M. Jarauta, Estibaliz Guallar, Eliseo Ibanez, Borja Civeira, Fernando TI Femoral and Carotid Subclinical Atherosclerosis Association With Risk Factors and Coronary Calcium The AWHS Study SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article DE cohort; computed tomography; risk assessment; ultrasonography ID INTIMA-MEDIA THICKNESS; PERIPHERAL ARTERIAL-DISEASE; HEART-DISEASE; INTERMITTENT CLAUDICATION; CARDIOVASCULAR EVENTS; MYOCARDIAL-INFARCTION; POST-MORTEM; PLAQUE; METAANALYSIS; PREVALENCE AB BACKGROUND Early subclinical atherosclerosis has been mainly researched in carotid arteries. The potential value of femoral arteries for improving the predictive capacity of traditional risk factors is an understudied area. OBJECTIVES This study sought to evaluate the association of subclinical carotid and femoral plaques with risk factors and coronary artery calcium score (CACS) in middle-aged men. METHODS Participants (n = 1,423) of the AWHS (Aragon Workers' Health Study), a study designed to assess cardiovascular risk and subclinical atherosclerosis in a cohort of middle-aged men (40 to 59 years of age), underwent carotid and femoral ultrasound plus noncontrast coronary computed tomography. Subclinical atherosclerosis was defined as the presence of any plaque in carotid or femoral arteries and/or CACS >= 1. Logistic regression models were used to estimate the prevalence of atherosclerosis adjusted for risk factors and age, to evaluate the association of atherosclerosis with risk factors, and to calculate areas under the receiver-operating characteristic curves for the presence of positive CACS. RESULTS Subclinical atherosclerosis was found in 72% of participants. Plaques were most common in femoral arteries (54%), followed by coronary calcification (38%) and carotid plaques (34%). Association of atherosclerosis with risk factors was stronger in femoral arteries than carotid or coronary arteries. The area under the receiver-operating characteristic curve for prediction of positive CACS increased from 0.665 when considering only risk factors (dyslipidemia, current smoking, hypertension, diabetes, and age) to 0.719 when adding femoral and carotid plaques (p < 0.001). In this model, the femoral odds ratio (2.58) exceeded the carotid odds ratio (1.80) for prediction of positive CACS. CONCLUSIONS Subclinical atherosclerosis was highly prevalent in this middle-aged male cohort. Association with risk factors and positive CACS was stronger in femoral than carotid arteries. Screening for femoral plaques may be an appealing strategy for improving cardiovascular risk scales and predicting coronary disease. (C) 2016 by the American College of Cardiology Foundation. C1 [Laclaustra, Martin; Fernandez-Ortiz, Antonio; Fuster, Valentin; Jimenez-Borreguero, Luis J.; Hurtado-Roca, Yamilee; Ordovas, Jose M.; Ibanez, Borja] Ctr Nacl Invest Cardiovasc Carlos III, Madrid, Spain. [Laclaustra, Martin] Univ Autonoma Madrid, CIBERESP, Sch Med, Dept Prevent Med & Publ Hlth, Madrid, Spain. [Laclaustra, Martin] St Louis Univ, Dept Epidemiol, St Louis, MO 63103 USA. [Casasnovas, Jose A.; Leon-Latre, Monserrat; Jarauta, Estibaliz; Civeira, Fernando] IIS Aragon, Inst Aragones Ciencias Salud, Zaragoza, Spain. [Fernandez-Ortiz, Antonio] Univ Complutense, Hosp Clin San Carlos, E-28040 Madrid, Spain. [Fuster, Valentin] Mt Sinai Sch Med, New York, NY USA. [Jimenez-Borreguero, Luis J.] Hosp Univ La Princesa, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. [Jarauta, Estibaliz; Civeira, Fernando] Hosp Univ Miguel Servet, IIS Aragon, Zaragoza, Spain. [Guallar, Eliseo] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Guallar, Eliseo] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Med, Baltimore, MD USA. [Guallar, Eliseo] Johns Hopkins Bloomberg Sch Publ Hlth, Welch Ctr Prevent Epidemiol & Clin Res, Baltimore, MD USA. [Ibanez, Borja] Univ Autonoma Madrid, Fdn Jimenez Diaz, Madrid, Spain. RP Fuster, V (reprint author), Icahn Sch Med Mt Sinai, Zena & Michael Wiener Cardiovasc Inst, One Gustave L Levy Pl,Box 1030, New York, NY 10029 USA. EM valentin.fustet@mountsinai.org RI Ibanez, Borja/J-6993-2014; Fuster, Valentin/H-4319-2015; Fernandez-Ortiz, Antonio/B-2227-2017 OI Ibanez, Borja/0000-0002-5036-254X; Fuster, Valentin/0000-0002-9043-9986; Fernandez-Ortiz, Antonio/0000-0002-3239-1910 FU Instituto Aragones de Ciencias de la Salud (I+CS); Spanish National Center for Cardiovascular Diseases (CNIC); Instituto de Salud Carlos III [FIS CP08/00112, PI10/00021, PI14/00009]; FEDER; FIS [PI12/01434, PI12/01703, PI12/01087]; FINCyT Science and Technology Program [088-FINCyT-BDE-2014, 1663/OC-PE]; Inter-American Development Bank; RETIC RIC [RD12/0042/0055]; Sanofi; Lilly; Synageva; Pfizer; Amgen; Merck FX The AWHS (Aragon Workers' Health Study) is funded by the Instituto Aragones de Ciencias de la Salud (I+CS) and the Spanish National Center for Cardiovascular Diseases (CNIC). Dr. Laclaustra was supported in part by grants FIS CP08/00112, PI10/00021, and PI14/00009 from the Instituto de Salud Carlos III, which includes FEDER funding. Dr. Leon-Latre was supported by FIS PI12/01434. Dr. Pocovi was supported by FIS PI12/01703. Dr. Hurtado-Roca was supported by FINCyT Science and Technology Program Scholarship No088-FINCyT-BDE-2014 from agreement 1663/OC-PE, between the Republic of Peru and the Inter-American Development Bank. Dr. Civeira was supported by FIS PI12/01087 and RETIC RIC RD12/0042/0055; has received personal fees from Sanofi, Lilly, Synageva, Pfizer, and Amgen; and has received grants and personal fees from Merck. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose. Tasneem Z. Naqvi, MD, served as Guest Editor for this paper. NR 37 TC 5 Z9 5 U1 2 U2 13 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0735-1097 EI 1558-3597 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 22 PY 2016 VL 67 IS 11 BP 1263 EP 1274 DI 10.1016/j.jacc.2015.12.056 PG 12 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA DG2FH UT WOS:000371881400001 PM 26988945 ER PT J AU Case, AE Mayfield, AE Clark, SL Schlarbaum, SE Reynolds, BC AF Case, Ashley E. Mayfield, Albert E., III Clark, Stacy L. Schlarbaum, Scott E. Reynolds, Barbara C. TI Abundance and Frequency of the Asiatic Oak Weevil (Coleoptera: Curculionidae) and Defoliation on American, Chinese, and Hybrid Chestnut (Castanea) SO JOURNAL OF INSECT SCIENCE LA English DT Article DE defoliation; Castanea dentata; Castanea mollissima; Cyrtepistomus castaneus; hybrid chestnut ID UNITED-STATES; RAIN-FOREST; DENTATA; RESISTANCE; DISEASE; GENERATIONS; HERBIVORY; INSECTS; QUALITY; DAMAGE AB The Asiatic oak weevil, Cyrtepistomus castaneus Roelofs (Coleoptera: Curculionidae), is a nonnative defoliator of trees in the Fagaceae family in the United States but has not been studied on Castanea species in the southern Appalachian Mountains. Planted trees of Castanea dentata (Marsh.) Borkh. (Fagales: Fagaceae), Castanea mollissima Blume (Fagales: Fagaceae), and four hybrid breeding generations were evaluated in 2012 for insect defoliation and C. castaneus abundance and frequency. Defoliation was visually assessed throughout the growing season at two sites in the southern Appalachian Mountains (western North Carolina and eastern Tennessee). C. castaneus abundance and frequency were monitored on trees using beat sheets and emergence was recorded from ground traps. Asiatic oak weevils were more abundant and more frequently collected on American chestnut (Ca. dentata) and its most closely related BC3F3 hybrid generation than on the Asian species Ca. mollissima. In most months, C. castaneus colonization of hybrid generations was not significantly different than colonization of parental species. Frequency data for C. castaneus suggested that adults were distributed relatively evenly throughout the study sites rather than in dense clusters. Emergence of C. castaneus was significantly higher under a canopy dominated by Quercus species than under non-Quercus species or open sky. C. castaneus emergence began in May and peaked in late June and early July. These results may be useful for resource managers trying to restore blight-resistant chestnut to the Southern Appalachians while minimizing herbivory by insect pests. C1 [Case, Ashley E.; Schlarbaum, Scott E.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, 274 Ellington Hall, Knoxville, TN 37996 USA. [Mayfield, Albert E., III] US Forest Serv, USDA, Southern Res Stn, 200WT Weaver Blvd, Asheville, NC 28804 USA. [Clark, Stacy L.] US Forest Serv, USDA, Southern Res Stn, 274 Ellington Hall, Knoxville, TN 37996 USA. [Reynolds, Barbara C.] Univ N Carolina, Dept Environm Studies, 155 Rhoades Robinson Hall, Asheville, NC 28804 USA. RP Mayfield, AE (reprint author), US Forest Serv, USDA, Southern Res Stn, 200WT Weaver Blvd, Asheville, NC 28804 USA. EM acase8@utk.edu; amayfield02@fs.fed.us; stacyclark@fs.fed.us; tenntip@utk.edu; kreynold@unca.edu FU USDA Forest Service, Southern Research Station [12-CA-11330129-026]; University of North Carolina, Asheville; University of Tennessee, Knoxville FX The following individuals and agencies are sincerely thanked for their technical assistance with this project: USDA Forest Service Region 8 staff for assistance in site selection, site preparation, and planting; Ashley Hancock, Andy Tait, and Dustin Neufeld (University of North Carolina Asheville) and John Johnson The University of Tennessee Tree Improvement Program; and Dr. Arnold M. Saxton at The University of Tennessee in Knoxville for statistical guidance. We would also like to acknowledge the cooperation of the Flint River Nursery, Georgia Forestry Commission, for cooperation in growing the seedlings for this study. This study was partially funded by the USDA Forest Service, Southern Research Station through cooperative agreement #12-CA-11330129-026 with the, University of North Carolina, Asheville with additional support from the University of Tennessee, Knoxville. NR 47 TC 0 Z9 0 U1 2 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD MAR 21 PY 2016 VL 16 AR 29 DI 10.1093/jisesa/iew012 PG 8 WC Entomology SC Entomology GA DJ9HU UT WOS:000374525400002 ER PT J AU Fageria, NK Baligar, VC AF Fageria, N. K. Baligar, V. C. TI Growth, yield and yield components of dry bean as influenced by phosphorus in a tropical acid soil SO JOURNAL OF PLANT NUTRITION LA English DT Article DE P harvest index; P use efficiency; Phaseolus vulgaris; grain harvest index ID LOWLAND RICE; INCEPTISOL AB Phosphorus (P) deficiency is one of the most yield limiting factors for dry bean (Phaseolus vulgaris) production in tropical acid soils. Dry beans are invariably grown as mono-crops or as inter-crops under the perennial tropical crops. Information is limited regarding the influence of phosphorus fertilization on dry bean yield and yield components and P use efficiency in tropical acid soils. A greenhouse experiment was conducted to evaluate the influence of phosphorus fertilization on dry bean growth, yield and yield components and P uptake parameters. Phosphorus rates used were 0, 50, 100, 150, 200, and 250mg P kg(-1) of soil. Soil used in the experiment was an acidic Inceptisol. Grain yield, shoot dry weight, number of pods, and 100 grain weight were significantly (P < 0.01) increased with phosphorus fertilization. Maximum grain yield, shoot dry matter, number of pods, and 100 grain weight were obtained with the application of 165, 216, 162, and 160mg P kg(-1) of soil, respectively, as calculated by regression equations. Grain yield was significantly and positively associated with shoot dry weight, number of pods, P concentration in grain and total uptake of P in shoot and grain. Phosphorus use efficiency defined in several ways, decreased with increasing P rates from 50 to 250mg P kg(-1) of soil. Maximum grain yield was obtained at 82mg kg(-1) of Mehlich 1 extractable soil P. Results suggest that dry bean yield in Brazilian Inceptisols could be significantly increased with the use of adequate rates of phosphorus fertilization. C1 [Fageria, N. K.] EMBRAPA, Natl Rice & Bean Res Ctr, Santo Antonio De Goias, Brazil. [Baligar, V. C.] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Baligar, VC (reprint author), USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM vc.baligar@ars.usda.gov NR 17 TC 0 Z9 0 U1 4 U2 6 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0190-4167 EI 1532-4087 J9 J PLANT NUTR JI J. Plant Nutr. PD MAR 20 PY 2016 VL 39 IS 4 BP 562 EP 568 DI 10.1080/01904167.2016.1143489 PG 7 WC Plant Sciences SC Plant Sciences GA DH3NR UT WOS:000372694200012 ER PT J AU Raczkowski, CW Kissel, DE Vigil, MF Cabrera, ML AF Raczkowski, Charles W. Kissel, David E. Vigil, Merle F. Cabrera, Miguel L. TI Fertilizer placement to maximize nitrogen use by fescue SO JOURNAL OF PLANT NUTRITION LA English DT Article DE fertilizer nitrogen recovery; Fertilizer application method; forage yield; nitrogen uptake ID TALL FESCUE; PLANT-GROWTH; PERFORMANCE; YIELD AB The method of fertilizer nitrogen (N) application can affect N uptake in tall fescue and therefore its yield and quality. Subsurface-banding (knife) of fertilizer maximizes fescue N uptake in the poorly-drained clay-pan soils of southeastern Kansas. This study was conducted to determine if knifed N results in greater N uptake than the conventional top-dress application method in a deep, well-drained soil of east-central Kansas. The experiment, conducted in a Smolan silty clay loam soil, was a split-plot with fertilizer nitrogen rates 0, 140 and 280kg N ha(-1) applied as urea-ammonium nitrate (UAN, 28% N), knifed or top-dressed. Soil inorganic N [ammonium (NH4)- and nitrate (NO3-N)] and N in roots and plant tops were measured at various times during the growing season. At final harvest, most of the knifed N (99.7%) was accounted for in plant tissue (roots and tops) and soil, with more than half of the knifed N remaining as soil inorganic N. With the top-dressed method, 27% was unaccounted for and presumed lost in gaseous form. Knifing fertilizer N in fescue fields of east-central Kansas will maximize the availability of N, reduce potential N losses, and increase forage quality. C1 [Raczkowski, Charles W.] North Carolina A&T State Univ, Dept Nat Resources, 1601 East Market St, Greensboro, NC 27411 USA. [Kissel, David E.; Cabrera, Miguel L.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Vigil, Merle F.] USDA ARS, Cent Plains Resources Management Res, Akron, OH USA. RP Raczkowski, CW (reprint author), North Carolina A&T State Univ, Dept Nat Resources, 1601 East Market St, Greensboro, NC 27411 USA. EM raczkowc@ncat.edu NR 10 TC 0 Z9 0 U1 3 U2 16 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0190-4167 EI 1532-4087 J9 J PLANT NUTR JI J. Plant Nutr. PD MAR 20 PY 2016 VL 39 IS 4 BP 581 EP 587 DI 10.1080/01904167.2016.1143491 PG 7 WC Plant Sciences SC Plant Sciences GA DH3NR UT WOS:000372694200014 ER PT J AU Kumari, S Deng, WP Gunasekara, C Chiang, V Chen, HS Ma, H Davis, X Wei, HR AF Kumari, Sapna Deng, Wenping Gunasekara, Chathura Chiang, Vincent Chen, Huann-sheng Ma, Hao Davis, Xin Wei, Hairong TI Bottom-up GGM algorithm for constructing multilayered hierarchical gene regulatory networks that govern biological pathways or processes SO BMC BIOINFORMATICS LA English DT Article DE Multilayered gene regulatory network; Pathway; Microarray or RNA-seq data ID NAC TRANSCRIPTION FACTORS; SECONDARY WALL SYNTHESIS; EXPRESSION DATA; ARABIDOPSIS-THALIANA; ESCHERICHIA-COLI; POPULUS-TRICHOCARPA; BAYESIAN NETWORKS; MICROARRAY DATA; WOOD FORMATION; FACTORS NST1 AB Background: Multilayered hierarchical gene regulatory networks (ML-hGRNs) are very important for understanding genetics regulation of biological pathways. However, there are currently no computational algorithms available for directly building ML-hGRNs that regulate biological pathways. Results: A bottom-up graphic Gaussian model (GGM) algorithm was developed for constructing ML-hGRN operating above a biological pathway using small-to medium-sized microarray or RNA-seq data sets. The algorithm first placed genes of a pathway at the bottom layer and began to construct a ML-hGRN by evaluating all combined triple genes: two pathway genes and one regulatory gene. The algorithm retained all triple genes where a regulatory gene significantly interfered two paired pathway genes. The regulatory genes with highest interference frequency were kept as the second layer and the number kept is based on an optimization function. Thereafter, the algorithm was used recursively to build a ML-hGRN in layer-by-layer fashion until the defined number of layers was obtained or terminated automatically. Conclusions: We validated the algorithm and demonstrated its high efficiency in constructing ML-hGRNs governing biological pathways. The algorithm is instrumental for biologists to learn the hierarchical regulators associated with a given biological pathway from even small-sized microarray or RNA-seq data sets. C1 [Kumari, Sapna; Deng, Wenping; Gunasekara, Chathura; Wei, Hairong] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Chiang, Vincent; Davis, Xin] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Chen, Huann-sheng] NCI, Stat Methodol & Applicat Branch, Div Canc Control & Populat Sci, NIH, Rockville, MD 20850 USA. [Ma, Hao] ARS, NCCWA, USDA, Kearneysville, WV 25430 USA. RP Wei, HR (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. EM hairong@mtu.edu NR 70 TC 1 Z9 1 U1 2 U2 7 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2105 J9 BMC BIOINFORMATICS JI BMC Bioinformatics PD MAR 18 PY 2016 VL 17 AR 132 DI 10.1186/s12859-016-0981-1 PG 12 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Mathematical & Computational Biology GA DH0PQ UT WOS:000372486500005 PM 26993098 ER PT J AU Garcia, RA Roemmich, JN Claycombe, KJ AF Garcia, Rolando A. Roemmich, James N. Claycombe, Kate J. TI Evaluation of markers of beige adipocytes in white adipose tissue of the mouse SO NUTRITION & METABOLISM LA English DT Article DE Beige; Brite; Adipocyte marker; Subcutaneous adipose tissue; UCP1; FGF21 ID FAT; BROWN; OBESITY; THERMOGENESIS; ABLATION; MICE AB Background: There is a growing interest in exploiting the induction of beige or "brite" (brown in white) adipocytes (beigeing) to combat obesity and its comorbidities. However, there is some uncertainty regarding the best markers to evaluate the occurrence or magnitude of beigeing in white adipose tissue in the mouse model. Methods: We evaluated the transcript expression of several thermoregulatory genes and proposed beige markers employing cell culture, whole white adipose tissue, and the adipocyte and stromal vascular fractions. Results: Most beige markers tested with the exception of TMEM26 can discriminate white from beige adipocytes in culture. Markers FGF21, P2RX5, PAT2, or CAR4 can successfully mark beigeing in whole tissue of younger mice, or in the adipocyte subfraction of older mice. However, markers for the thermoregulatory genes UCP1, CIDEA, and Cox8b displayed the greatest dynamic range and were consistently elevated in vitro, in vivo, and in the adipocyte fraction by treatments that induce beige adipogenesis. Conclusions: While most putative beige markers are clearly expressed in beige adipocytes in vitro, in vivo the small dynamic range of most of these markers, the strength of the beigeing stimulus, and the age of the mice may limit their utility, although this limitation may be overcome by specifically evaluating these markers in the adipocyte fraction. Thermoregulatory markers like UCP1, CIDEA, or Cox8b represent the best options to evaluate the beigeing of white adipose tissue in vivo. C1 [Garcia, Rolando A.; Roemmich, James N.; Claycombe, Kate J.] USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. RP Garcia, RA (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. EM rolando.garcia@ars.usda.gov FU USDA Agricultural Research Service Project [3062-51000-052-00D] FX This work was supported by USDA Agricultural Research Service Project #3062-51000-052-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 20 TC 2 Z9 2 U1 6 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1743-7075 J9 NUTR METAB JI Nutr. Metab. PD MAR 18 PY 2016 VL 13 AR 24 DI 10.1186/s12986-016-0081-2 PG 14 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DH1YS UT WOS:000372581500001 PM 26997968 ER PT J AU Park, JB AF Park, Jae B. TI Finding Potent Sirt Inhibitor in Coffee: Isolation, Confirmation and Synthesis of Javamide-II (N-Caffeoyltryptophan) as Sirt1/2 Inhibitor SO PLOS ONE LA English DT Article ID FAMILIAL ALZHEIMERS-DISEASE; PROSTATE-CANCER; CRYSTAL-STRUCTURE; CONSUMPTION; MECHANISM; RISK; ACETYLATION; COMPLEX; BETA; TAU AB Recent studies suggest that Sirt inhibition may have beneficial effects on several human diseases such as neurodegenerative diseases and cancer. Coffee is one of most popular beverages with several positive health effects. Therefore, in this paper, potential Sirt inhibitors were screened using coffee extract. First, HPLC was utilized to fractionate coffee extract, then screened using a Sirt1/2 inhibition assay. The screening led to the isolation of a potent Sirt1/2 inhibitor, whose structure was determined as javamide-II (N-caffeoyltryptophan) by NMR. For confirmation, the amide was chemically synthesized and its capacity of inhibiting Sirt1/2 was also compared with the isolated amide. Javamide-II inhibited Sirt2 (IC50; 8.7 mu M) better than Sirt1(IC50; 34 mu M). Since javamide-II is a stronger inhibitor for Sirt2 than Sirt1. The kinetic study was performed against Sirt2. The amide exhibited noncompetitive Sirt2 inhibition against the NAD(+) (K-i = 9.8 mu M) and showed competitive inhibition against the peptide substrate (K-i = 5.3 mu M). Also, a docking simulation showed stronger binding pose of javamide-II to Sirt2 than AGK2. In cellular levels, javamide-II was able to increase the acetylation of total lysine, cortactin and histone H3 in neuronal NG108-15 cells. In the same cells, the amide also increased the acetylation of lysine (K382) in p53, but not (K305). This study suggests that Javamide-II found in coffee may be a potent Sirt1/2 inhibitor, probably with potential use in some conditions of human diseases. C1 [Park, Jae B.] ARS, Diet Genom & Immunol Lab, BHNRC, USDA, Bldg 307C,Rm 131, Beltsville, MD 20705 USA. RP Park, JB (reprint author), ARS, Diet Genom & Immunol Lab, BHNRC, USDA, Bldg 307C,Rm 131, Beltsville, MD 20705 USA. EM jae.park@ars.usda.gov FU ARS/USDA grant [8040-51000-057-00] FX This study was supported by an ARS/USDA grant (project number 8040-51000-057-00). The funder had no role in study design, data collection and analysis, decision to publish or preparation of manuscript. NR 45 TC 1 Z9 1 U1 5 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 17 PY 2016 VL 11 IS 3 AR e0150392 DI 10.1371/journal.pone.0150392 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH1YG UT WOS:000372580300020 PM 26986569 ER PT J AU Shinneman, DJ Means, RE Potter, KM Hipkins, VD AF Shinneman, Douglas J. Means, Robert E. Potter, Kevin M. Hipkins, Valerie D. TI Exploring Climate Niches of Ponderosa Pine (Pinus ponderosa Douglas ex Lawson) Haplotypes in the Western United States: Implications for Evolutionary History and Conservation SO PLOS ONE LA English DT Article ID SPECIES DISTRIBUTION MODELS; GENETIC-STRUCTURE; NORTH-AMERICA; MITOCHONDRIAL-DNA; ICE-AGES; USA; POPULATION; FOREST; CONSEQUENCES; POLLEN AB Ponderosa pine (Pinus ponderosa Douglas ex Lawson) occupies montane environments throughout western North America, where it is both an ecologically and economically important tree species. A recent study using mitochondrial DNA analysis demonstrated substantial genetic variation among ponderosa pine populations in the western U.S., identifying 10 haplotypes with unique evolutionary lineages that generally correspond spatially with distributions of the Pacific (P. p. var. ponderosa) and Rocky Mountain (P. p. var. scopulorum) varieties. To elucidate the role of climate in shaping the phylogeographic history of ponderosa pine, we used nonparametric multiplicative regression to develop predictive climate niche models for two varieties and 10 haplotypes and to hindcast potential distribution of the varieties during the last glacial maximum (LGM), similar to 22,000 yr BP. Our climate niche models performed well for the varieties, but haplotype models were constrained in some cases by small datasets and unmeasured microclimate influences. The models suggest strong relationships between genetic lineages and climate. Particularly evident was the role of seasonal precipitation balance in most models, with winter- and summer-dominated precipitation regimes strongly associated with P. p. vars. ponderosa and scopulorum, respectively. Indeed, where present-day climate niches overlap between the varieties, introgression of two haplotypes also occurs along a steep clinal divide in western Montana. Reconstructed climate niches for the LGM suggest potentially suitable climate existed for the Pacific variety in the California Floristic province, the Great Basin, and Arizona highlands, while suitable climate for the Rocky Mountain variety may have existed across the southwestern interior highlands. These findings underscore potentially unique phylogeographic origins of modern ponderosa pine evolutionary lineages, including potential adaptations to Pleistocene climates associated with discrete temporary glacial refugia. Our predictive climate niche models may inform strategies for further genetic research (e.g., sampling design) and conservation that promotes haplotype compatibility with projected changes in future climate. C1 [Shinneman, Douglas J.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID USA. [Means, Robert E.] Bur Land Management Wyoming, Cheyenne, WY USA. [Potter, Kevin M.] N Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC USA. [Hipkins, Valerie D.] US Forest Serv, Natl Forest Genet Lab, USDA, Placerville, CA USA. RP Shinneman, DJ (reprint author), US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID USA. EM dshinneman@usgs.gov OI Potter, Kevin/0000-0002-7330-5345 FU Southern Research Station of the USDA Forest Service [14-CS-11330110-042, 15-CS-11330110-067]; North Carolina State University [14-CS-11330110-042, 15-CS-11330110-067]; U.S. Bureau of Land Management [L12PG00219]; U.S. Geological Survey [L12PG00219] FX This work was supported in part through Cost Share Agreements 14-CS-11330110-042 and 15-CS-11330110-067 between the Southern Research Station of the USDA Forest Service and North Carolina State University (Kevin Potter). Funding for developing the climate niche models presented in this paper was provided through an Interagency Agreement (L12PG00219) between the U.S. Bureau of Land Management and the U.S. Geological Survey (Douglas Shinneman). Robert Means (BLM) helped to collect and analyze data, prepare the manuscript, and was involved in the decision to publish. NR 71 TC 1 Z9 1 U1 7 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 17 PY 2016 VL 11 IS 3 AR e0151811 DI 10.1371/journal.pone.0151811 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH1YG UT WOS:000372580300134 PM 26985674 ER PT J AU Venkatesh, TV Chassy, AW Fiehn, O Flint-Garcia, S Zeng, Q Skogerson, K Harrigan, GG AF Venkatesh, Tyamagondlu V. Chassy, Alexander W. Fiehn, Oliver Flint-Garcia, Sherry Zeng, Qin Skogerson, Kirsten Harrigan, George G. TI Metabolomic Assessment of Key Maize Resources: GC-MS and NMR Profiling of Grain from B73 Hybrids of the Nested Association Mapping (NAM) Founders and of Geographically Diverse Landraces SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE maize hybrids; nested association mapping (NAM) founders; landraces; metabolomics; GC-TOF-MS; NMR ID CONVENTIONAL MAIZE; TRANSGENIC MAIZE; ENVIRONMENT; POPULATION; GENETICS; IMPROVEMENT; PREDICTION; VARIETIES; KERNELS; TRAITS AB The present study expands metabolomic assessments of maize beyond commercial lines to include two sets of hybrids used extensively in the scientific community. One set included hybrids derived from the nested association mapping (NAM) founder lines, a collection of 25 inbreds selected on the basis of genetic diversity and used to investigate the genetic basis of complex plant traits. A second set included 24 hybrids derived from a collection of Landraces representative of native diversity from North and South America that may serve as a source of new alleles for improving modern maize hybrids. Metabolomic analysis of grain harvested from these hybrids utilized gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) and H-1 nuclear magnetic resonance spectroscopy (H-1 NMR) techniques. Results highlighted extensive metabolomic variation in grain from both hybrid sets, but also demonstrated that, within each hybrid set, subpopulations could be differentiated in a pattern consistent with the known genetic and compositional variation of these lines. Correlation analysis did not indicate a strong association of the metabolomic data with grain nutrient composition, although some metabolites did show moderately strong correlations with agronomic features such as plant and ear height. Overall, this study provides insights into the extensive metabolomic diversity associated with conventional maize germplasm. C1 [Venkatesh, Tyamagondlu V.; Zeng, Qin; Skogerson, Kirsten; Harrigan, George G.] Monsanto Co, 800 N Lindbergh Blvd, St Louis, MO 63167 USA. [Chassy, Alexander W.; Fiehn, Oliver] Univ Calif Davis, Genome Ctr Metabol, Davis, CA 95616 USA. [Fiehn, Oliver] King Abudalaziz Univ, Dept Biochem, Jeddah, Saudi Arabia. [Flint-Garcia, Sherry] ARS, USDA, Columbia, MO 65211 USA. [Flint-Garcia, Sherry] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Venkatesh, TV (reprint author), Monsanto Co, 800 N Lindbergh Blvd, St Louis, MO 63167 USA.; Flint-Garcia, S (reprint author), ARS, USDA, Columbia, MO 65211 USA.; Flint-Garcia, S (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM tvvenkatesh@gmail.com; Sherry.Flint-Garcia@ars.usda.gov NR 48 TC 3 Z9 3 U1 6 U2 20 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 16 PY 2016 VL 64 IS 10 BP 2162 EP 2172 DI 10.1021/acs.jafc.5b04901 PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DH0MM UT WOS:000372478300016 PM 26923484 ER PT J AU Herlihy, MV Talamas, EJ Weber, DC AF Herlihy, Megan V. Talamas, Elijah J. Weber, Donald C. TI Attack and Success of Native and Exotic Parasitoids on Eggs of Halyomorpha halys in Three Maryland Habitats SO PLOS ONE LA English DT Article ID PODISUS-MACULIVENTRIS HEMIPTERA; MARMORATED STINK BUG; ASHMEAD HYMENOPTERA; NATURAL ENEMIES; NORTH-AMERICA; PENTATOMIDAE; HETEROPTERA; SCELIONIDAE; ATTRACTION; MORTALITY AB Egg parasitoids of the exotic invasive brown marmorated stink bug, Halyomorpha halys (Stal), were investigated using lab-reared fresh (live) and frozen (killed) lab-reared sentinel egg masses deployed for 72h on foliage in three habitats-woods, orchard, and soybean field-in Maryland, USA, in summer 2014. Four native hymenopteran species, Telenomus podisi Ashmead (Scelionidae), Trissolcus euschisti (Ashmead) and Tr. brochymenae Ashmead (Scelionidae), and Anastatus reduvii (Howard) (Eupelmidae), developed and emerged from H. halys eggs. One exotic parasitoid, Trissolcus japonicus (Ashmead), emerged, providing the first known occurrence of this species in North America. Native parasitoids emerged from frozen eggs significantly more often than from fresh eggs (89.3% of egg masses and 98.1% of individual eggs), whereas the exotic Tr. japonicus did not show a similar difference, strongly suggesting adaptation to H. halys as a host by Tr. japonicus but not by the native species. Parasitoids were habitat-specific: all three Trissolcus species were significantly more likely to occur in the woods habitat, whereas Te. podisi was found exclusively in the soybean field. Further investigations are required to elucidate evolving host-parasitoid relationships, habitat specificity, and non-target effects of Tr. japonicus over the expanded range of H. halys in North America. C1 [Herlihy, Megan V.; Weber, Donald C.] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD USA. [Talamas, Elijah J.] ARS, Systemat Entomol Lab, USDA, Washington, DC USA. [Talamas, Elijah J.] Smithsonian Inst, Washington, DC 20560 USA. RP Weber, DC (reprint author), ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD USA. EM Don.Weber@ars.usda.gov FU Systematic Entomology Laboratory; Invasive Insect Biocontrol and Behavior Laboratory, USDA ARS FX This work was made possible by funding from the Systematic Entomology Laboratory and the Invasive Insect Biocontrol and Behavior Laboratory, USDA ARS. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer. NR 25 TC 5 Z9 5 U1 14 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 16 PY 2016 VL 11 IS 3 AR e0150275 DI 10.1371/journal.pone.0150275 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH1WF UT WOS:000372574900030 PM 26983012 ER PT J AU Wen, CL Cheng, Q Zhao, LQ Mao, AJ Yang, JJ Yu, SC Weng, YQ Xu, Y AF Wen, Chang-long Cheng, Qing Zhao, Liqun Mao, Aijun Yang, Jingjing Yu, Shuancang Weng, Yiqun Xu, Yong TI Identification and characterisation of Dof transcription factors in the cucumber genome SO SCIENTIFIC REPORTS LA English DT Article ID ARABIDOPSIS-THALIANA; FACTOR FAMILY; DNA-BINDING; GENE FAMILIES; WIDE ANALYSIS; SATIVUS L; RESISTANCE; EXPRESSION; GERMINATION; PLANTS AB Cucumber is vulnerable to many foliage diseases. Recent studies reported cloning of candidate genes for several diseases in cucumber; however, the exact defence mechanisms remain unclear. Dof genes have been shown to play significant roles in plant growth, development, and responses to biotic and abiotic stresses. Dof genes coding for plant-specific transcription factors can promote large-scale expression of defence-related genes at whole genome level. The genes in the family have been identified and characterized in several plant species, but not in cucumber. In the present study, we identified 36 CsDof members from the cucumber draft genomes which could be classified into eight groups. The proportions of the CsDof family genes, duplication events, chromosomal locations, cis-elements and miRNA target sites were comprehensively investigated. Consequently, we analysed the expression patterns of CsDof genes in specific tissues and their response to two biotic stresses (watermelon mosaic virus and downy mildew). These results indicated that CsDof may be involved in resistance to biotic stresses in cucumber. C1 [Wen, Chang-long; Cheng, Qing; Mao, Aijun; Yang, Jingjing; Yu, Shuancang; Xu, Yong] Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, BVRC, Beijing 100097, Peoples R China. [Wen, Chang-long; Cheng, Qing; Mao, Aijun; Yang, Jingjing; Yu, Shuancang; Xu, Yong] Beijing Key Lab Vegetable Germplasms Improvement, Beijing 100097, Peoples R China. [Zhao, Liqun] Beijing Agr Extens Stn, Beijing 100029, Peoples R China. [Weng, Yiqun] Univ Wisconsin, USDA ARS, Dept Hort, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Wen, CL (reprint author), Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, BVRC, Beijing 100097, Peoples R China.; Wen, CL (reprint author), Beijing Key Lab Vegetable Germplasms Improvement, Beijing 100097, Peoples R China. EM wenchanglong@nercv.org FU National Science & Technology Pillar Program [2015BAD02B00, 2014BAD01B09, 2012BAD02B03]; National Natural Science Foundation of China [31401873]; Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Human Resources and Social Security of the People Republic of China; Beijing Municipal Human Resource and Social Security Bureau; Beijing Academy of Agricultural and Forestry Sciences [GJHZ201505/QNJJ201501] FX This work was supported in part by grants from the National Science & Technology Pillar Program (2015BAD02B00, 2014BAD01B09 and 2012BAD02B03), the National Natural Science Foundation of China (No. 31401873), the Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Human Resources and Social Security of the People Republic of China, the Beijing Municipal Human Resource and Social Security Bureau, and the Beijing Academy of Agricultural and Forestry Sciences (GJHZ201505/QNJJ201501). NR 47 TC 3 Z9 4 U1 9 U2 25 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 16 PY 2016 VL 6 AR 23072 DI 10.1038/srep23072 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG4RJ UT WOS:000372059800001 PM 26979661 ER PT J AU Shen, Y Barros, M Vennemann, T Gallagher, DT Yin, Y Linden, SB Heselpoth, RD Spencer, DJ Donovan, DM Moult, J Fischetti, VA Heinrich, F Losche, M Nelson, DC AF Shen, Yang Barros, Marilia Vennemann, Tarek Ggallagher, D. Travis Yin, Yizhou Linden, Sara B. Heselpoth, Ryan D. Spencer, Dennis J. Donovan, David M. Moult, John Fischetti, Vincent A. Heinrich, Frank Losche, Mathias Nelson, Daniel C. TI A bacteriophage endolysin that eliminates intracellular streptococci SO ELIFE LA English DT Article ID BILAYER-LIPID MEMBRANES; RESISTANT STAPHYLOCOCCUS-AUREUS; CELL-PENETRATING PEPTIDE; GROUP-A STREPTOCOCCI; ELECTROSTATIC INTERACTION; ANTI-INFECTIVES; ANNEXIN A5; PHOSPHATIDYLSERINE; PYOGENES; LYSIN AB PIyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PIyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PIyC holoenzyme, mediated by its PIyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using model membranes establish that PIyCB interacts strongly with phosphatidylserine (PS), whereas its interaction with other lipids is weak, suggesting specificity for PS as its cellular receptor. Neutron reflection further substantiates that PIyC penetrates bilayers above a PS threshold concentration. Crystallography and docking studies identify key residues that mediate PIyCB PS interactions, which are validated by site-directed mutagenesis. This is the first report that a native endolysin can traverse epithelial membranes, thus substantiating the potential of PIyC as an antimicrobial for Spy in the extracellular and intracellular milieu and as a scaffold for engineering other functionalities. C1 [Shen, Yang; Ggallagher, D. Travis; Yin, Yizhou; Linden, Sara B.; Heselpoth, Ryan D.; Moult, John; Nelson, Daniel C.] Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD USA. [Barros, Marilia; Vennemann, Tarek; Heinrich, Frank; Losche, Mathias] Carnegie Mellon Univ, Dept Phys, Pittsburgh, PA 15213 USA. [Ggallagher, D. Travis] NIST, Gaithersburg, MD 20899 USA. [Spencer, Dennis J.; Fischetti, Vincent A.] Rockefeller Univ, Lab Bacterial Pathogenesis & Immunol, 1230 York Ave, New York, NY 10021 USA. [Donovan, David M.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD USA. [Moult, John] Univ Maryland, Dept Cell Biol & Mol Genet, Rockville, MD USA. [Heinrich, Frank; Losche, Mathias] NIST, Ctr Neutron Res, Gaithersburg, MD 20899 USA. [Losche, Mathias] Carnegie Mellon Univ, Dept Biomed Engn, Pittsburgh, PA 15213 USA. [Nelson, Daniel C.] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. RP Nelson, DC (reprint author), Univ Maryland, Inst Biosci & Biotechnol Res, Rockville, MD USA.; Nelson, DC (reprint author), Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. EM nelsond@umd.edu RI Heinrich, Frank/A-5339-2010; Losche, Mathias/J-2986-2013 OI Heinrich, Frank/0000-0002-8579-553X; Losche, Mathias/0000-0001-6666-916X FU National Institute of General [R01GM110202, R01GM101647]; National Institute of Allergy [R01A111822] FX National Institute of General R01GM110202 Daniel C Nelson Medical Sciencesr National Institute of Allergy R01A111822 Vincent A Fischetti and Infectious Diseasesr National Institute of General R01GM101647 Mathias Losche Medical Sciencesr The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. NR 66 TC 4 Z9 4 U1 4 U2 9 PU ELIFE SCIENCES PUBLICATIONS LTD PI CAMBRIDGE PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND SN 2050-084X J9 ELIFE JI eLife PD MAR 15 PY 2016 VL 5 AR e13152 DI 10.7554/eLife.13152 PG 26 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DK9DE UT WOS:000375229900001 ER PT J AU Nouwakpo, SK Weltz, MA McGwire, K AF Nouwakpo, Sayjro Kossi Weltz, Mark A. McGwire, Kenneth TI Assessing the performance of structure-from-motion photogrammetry and terrestrial LiDAR for reconstructing soil surface microtopography of naturally vegetated plots SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article DE photogrammetry; Structure-from-Motion; SfM; LiDAR; TLS; soil microtopography; DEM; soil erosion ID RANGE DIGITAL PHOTOGRAMMETRY; LASER SCANNER; AERIAL-PHOTOGRAPHY; DEPRESSION-STORAGE; ELEVATION MODELS; GULLY EROSION; ROUGHNESS; TOPOGRAPHY; RAINFALL; MICRORELIEF AB Soil microtopography is a property of critical importance in many earth surface processes but is often difficult to quantify. Advances in computer vision technologies have made image-based three-dimensional (3D) reconstruction or Structure-from-Motion (SfM) available to many scientists as a low cost alternative to laser-based systems such as terrestrial laser scanning (TLS). While the performance of SfM at acquiring soil surface microtopography has been extensively compared to that of TLS on bare surfaces, little is known about the impact of vegetation on reconstruction performance. This article evaluates the performance of SfM and TLS technologies at reconstructing soil microtopography on 6m x 2m erosion plots with vegetation cover ranging from 0% to 77%. Results show that soil surface occlusion by vegetation was more pronounced with TLS compared to SfM, a consequence of the single viewpoint laser scanning strategy adopted in this study. On the bare soil surface, elevation values estimated with SfM were within 5mm of those from TLS although long distance deformations were observed with the former technology. As vegetation cover increased, agreement between SfM and TLS slightly degraded but was significantly affected beyond 53% of ground cover. Detailed semivariogram analysis on meter-square-scale surface patches showed that TLS and SfM surfaces were very similar even on highly vegetated plots but with fine scale details and the dynamic elevation range smoothed out with SfM. Errors in the TLS data were mainly caused by the distance measurement function of the instrument especially at the fringe of occlusion regions where the laser beam intersected foreground and background features simultaneously. From this study, we conclude that a realistic approach to digitizing soil surface microtopography in field conditions can be implemented by combining strengths of the image-based method (simplicity and effectiveness at reconstructing soil surface under sparse vegetation) with the high accuracy of TLS-like technologies. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Nouwakpo, Sayjro Kossi] Univ Nevada, Nat Resources & Environm Sci, 1664 North Virginia St, Reno, NV 89557 USA. [Weltz, Mark A.] USDA ARS, Exot & Invas Weeds Res Unit, Reno, NV USA. [McGwire, Kenneth] Desert Res Inst Earth & Ecosyst Sci, Reno, NV USA. RP Nouwakpo, SK (reprint author), Univ Nevada, Nat Resources & Environm Sci, 1664 North Virginia St, Reno, NV 89557 USA. EM snouwakpo@unr.edu NR 63 TC 6 Z9 6 U1 17 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD MAR 15 PY 2016 VL 41 IS 3 BP 308 EP 322 DI 10.1002/esp.3787 PG 15 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DG8VJ UT WOS:000372361400002 ER PT J AU Taylor, EB Moulana, M Stuge, TB Quiniou, SMA Bengten, E Wilson, M AF Taylor, Erin B. Moulana, Mohadetheh Stuge, Tor B. Quiniou, Sylvie M. A. Bengten, Eva Wilson, Melanie TI A Leukocyte Immune-Type Receptor Subset Is a Marker of Antiviral Cytotoxic Cells in Channel Catfish, Ictalurus punctatus SO JOURNAL OF IMMUNOLOGY LA English DT Article ID HEMORRHAGIC SEPTICEMIA VIRUS; GINBUNA CRUCIAN CARP; CYTO-TOXIC CELLS; NK-LIKE CELLS; MONOCLONAL-ANTIBODIES; PERIPHERAL-BLOOD; LYMPHOCYTE HETEROGENEITY; RAINBOW-TROUT; MEDIATED CYTOTOXICITY; ONCORHYNCHUS-MYKISS AB Channel catfish, Ictalurus punctatus, leukocyte immune type receptors (LITRs) represent a multigene family that encodes Ig superfamily proteins that mediate activating or inhibitory signaling. In this study, we demonstrate the use of mAb CC41 to monitor viral cytotoxic responses in catfish and determine that CC41 binds to a subset of LITRs on the surface of catfish clonal CTLs. Homozygous gynogenetic catfish were immunized with channel catfish virus (CCV)-infected MHC-matched clonal T cells (G14D-CCV), and PBL were collected at various times after immunization for flow cytometric analyses. The percentage of CC41(+) cells was significantly increased 5 d after primary immunization with G14D-CCV and at 3 d after a booster immunization as compared with control fish only injected with G14D. Moreover, CC41(+) cells magnetically isolated from the PBL specifically killed CCV-infected targets as measured by Cr-51 release assays and expressed messages for CD3 gamma delta, perforin, and at least one of the CD4-like receptors as analyzed by RNA flow cytometry. When MLC effector cells derived from a G14D-CCV-immunized fish were preincubated with CC41 mAb, killing of G14D-CCV targets was reduced by similar to 40%, suggesting that at least some LITRs have a role in target cell recognition and/or cytotoxicity. The availability of a LITR-specific mAb has allowed, to our knowledge for the first time, functional characterization of LITRs in an autologous system. In addition, the identification of an LITR subset as a cytotoxic cell marker will allow for more effective monitoring of catfish immune responses to pathogens. C1 [Taylor, Erin B.; Bengten, Eva; Wilson, Melanie] Univ Mississippi, Med Ctr, Dept Microbiol & Immunol, Jackson, MS 39216 USA. [Moulana, Mohadetheh; Quiniou, Sylvie M. A.] ARS, Warmwater Aquaculture Res Unit, USDA, Stoneville, MS 38776 USA. [Stuge, Tor B.] Arctic Univ Norway, Univ Tromso, Fac Hlth Sci, Immunol Res Grp,Dept Med Biol, N-9037 Tromso, Norway. [Moulana, Mohadetheh] Univ Mississippi, Med Ctr, Psychiat & Human Behav, Jackson, MS 39216 USA. RP Wilson, M (reprint author), Univ Mississippi, Med Ctr, 2500 North State St, Jackson, MS 39216 USA. EM mwilson@umc.edu OI Stuge, Tor B/0000-0002-6933-8419 FU U.S. Department of Agriculture [2009-65119-05672, 2010-65121-20649]; U.S. Department of Agriculture-National Institute of Food and Agriculture Predoctoral Fellowship [2013-67011-21019]; University of Mississippi Medical Center Intramural Research Support Program FX This work was supported by U.S. Department of Agriculture Grants 2009-65119-05672 and 2010-65121-20649 (to E.B. and M.W.), by U.S. Department of Agriculture-National Institute of Food and Agriculture Predoctoral Fellowship 2013-67011-21019 (to E.B.T.), and by University of Mississippi Medical Center Intramural Research Support Program 2015 (to M.W.). NR 73 TC 2 Z9 2 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 2016 VL 196 IS 6 BP 2677 EP 2689 DI 10.4049/jimmunol.1502166 PG 13 WC Immunology SC Immunology GA DG8MK UT WOS:000372338100025 PM 26856701 ER PT J AU Moges, AD Admassu, B Belew, D Yesuf, M Njuguna, J Kyalo, M Ghimire, SR AF Moges, Asmare D. Admassu, Belayneh Belew, Derbew Yesuf, Mohammed Njuguna, Joyce Kyalo, Martina Ghimire, Sita R. TI Development of Microsatellite Markers and Analysis of Genetic Diversity and Population Structure of Colletotrichum gloeosporioides from Ethiopia SO PLOS ONE LA English DT Article ID SIMPLE SEQUENCE REPEATS; MULTILOCUS GENOTYPE DATA; PATHOGENIC RUST FUNGUS; TANDEM REPEATS; LINKAGE MAP; EUKARYOTIC GENOMES; PUCCINIA-GRAMINIS; CAUSAL AGENT; SATIVA L.; DNA AB Twenty three polymorphic microsatellite markers were developed for citrus plant pathogenic fungus, Colletotrichum gloeosporioides, and were used to analyze genetic diversity and population structure of 163 isolates from four different geographical regions of Ethiopia. These loci produced a total of 118 alleles with an average of 5.13 alleles per microsatellite marker. The polymorphic information content values ranged from 0.104 to 0.597 with an average of 0.371. The average observed heterozygosity across all loci varied from 0.046 to 0.058. The gene diversity among the loci ranged from 0.106 to 0.664. Unweighted Neighbor-joining and population structure analysis grouped these 163 isolates into three major groups. The clusters were not according to the geographic origin of the isolates. Analysis of molecular variance showed 85% of the total variation within populations and only 5% among populations. There was low genetic differentiation in the total populations (FST = 0.049) as evidenced by high level of gene flow estimate (N-m = 4.8 per generation) among populations. The results show that Ethiopian C. gloeosporioides populations are generally characterized by a low level of genetic diversity. The newly developed microsatellite markers were useful in analyzing the genetic diversity and population structure of the C. gloeosporioides populations. Information obtained from this study could be useful as a base to design strategies for better management of leaf and fruit spot disease of citrus in Ethiopia. C1 [Moges, Asmare D.; Yesuf, Mohammed] Ethiopian Inst Agr Res, Dept Hort, Adama, Ethiopia. [Admassu, Belayneh] Ethiopian Inst Agr Res, Dept Agr Biotechnol, Holetta, Ethiopia. [Moges, Asmare D.; Belew, Derbew] Jimma Univ, Dept Hort & Plant Sci, Coll Agr & Vet Med, Jimma, Ethiopia. [Moges, Asmare D.; Njuguna, Joyce; Kyalo, Martina; Ghimire, Sita R.] Int Livestock Res Inst, Biosci Eastern & Cent Africa Hub, Nairobi, Kenya. [Admassu, Belayneh] USDA ARS, Aberdeen, MD USA. RP Ghimire, SR (reprint author), Int Livestock Res Inst, Biosci Eastern & Cent Africa Hub, Nairobi, Kenya. EM S.ghimire@cgiar.org FU BecA-ILRI Hub through the Africa Biosciences Challenge Fund (ABCF) program; Australian Department for Foreign Affairs and Trade (DFAT) through the BecA-CSIRO partnership; Syngenta Foundation for Sustainable Agriculture (SFSA); Bill & Melinda Gates Foundation (BMGF); UK Department for International Development (DFID); Swedish International Development Cooperation Agency (Sida) FX This project was supported by the BecA-ILRI Hub through the Africa Biosciences Challenge Fund (ABCF) program. The ABCF Program is funded by the Australian Department for Foreign Affairs and Trade (DFAT) through the BecA-CSIRO partnership; the Syngenta Foundation for Sustainable Agriculture (SFSA); the Bill & Melinda Gates Foundation (BMGF); the UK Department for International Development (DFID) and; the Swedish International Development Cooperation Agency (Sida), recipient: ADM. The funder had no role in preparation of the manuscript and the decision to publish. NR 121 TC 0 Z9 0 U1 11 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 15 PY 2016 VL 11 IS 3 AR e0151257 DI 10.1371/journal.pone.0151257 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH1VK UT WOS:000372572800054 PM 26978654 ER PT J AU Bouskill, NJ Wood, TE Baran, R Hao, Z Ye, Z Bowen, BP Lim, HC Nico, PS Holman, HY Gilbert, B Silver, WL Northen, TR Brodie, EL AF Bouskill, Nicholas J. Wood, Tana E. Baran, Richard Hao, Zhao Ye, Zaw Bowen, Ben P. Lim, Hsiao Chien Nico, Peter S. Holman, Hoi-Ying Gilbert, Benjamin Silver, Whendee L. Northen, Trent R. Brodie, Eoin L. TI Belowground Response to Drought in a Tropical Forest Soil. II. Change in Microbial Function Impacts Carbon Composition SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE tropical forest; drought; microbial functions; carbon dioxide; soil carbon ID DISSOLVED ORGANIC-MATTER; RAIN-FOREST; BACTERIAL COMMUNITIES; ANTIBIOTIC PRODUCTION; FTIR SPECTROSCOPY; IRON REDUCTION; DIOXIDE LOSSES; CLIMATE; MODELS; MICROORGANISMS AB Climate model projections for tropical regions show clear perturbation of precipitation patterns leading to increased frequency and severity of drought in some regions. Previous work has shown declining soil moisture to be a strong driver of changes in microbial trait distribution, however, the feedback of any shift in functional potential on ecosystem properties related to carbon cycling are poorly understood. Here we show that drought-induced changes in microbial functional diversity and activity shape, and are in turn shaped by, the composition of dissolved and soil-associated carbon. We also demonstrate that a shift in microbial functional traits that favor the production of hygroscopic compounds alter the efflux of carbon dioxide following soil rewetting. Under drought the composition of the dissolved organic carbon pool changed in a manner consistent with a microbial metabolic response. We hypothesize that this microbial ecophysiological response to changing soil moisture elevates the intracellular carbon demand stimulating extracellular enzyme production, that prompts the observed decline in more complex carbon compounds (e.g., cellulose and lignin). Furthermore, a metabolic response to drought appeared to condition (biologically and physically) the soil, notably through the production of polysaccharides, particularly in experimental plots that had been pre-exposed to a short-term drought. This hysteretic response, in addition to an observed drought-related decline in phosphorus concentration, may have been responsible for a comparatively modest CO2 efflux following wet-up in drought plots relative to control plots. C1 [Bouskill, Nicholas J.; Hao, Zhao; Ye, Zaw; Lim, Hsiao Chien; Nico, Peter S.; Holman, Hoi-Ying; Gilbert, Benjamin; Brodie, Eoin L.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Climate & Ecosyst Sci, Earth & Environm Sci, Berkeley, CA 94720 USA. [Wood, Tana E.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR USA. [Wood, Tana E.] Fdn Puertorriquena Conservac, San Juan, PR USA. [Baran, Richard; Bowen, Ben P.; Northen, Trent R.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol, Div Life Sci, Berkeley, CA 94720 USA. [Silver, Whendee L.; Brodie, Eoin L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Bouskill, NJ; Brodie, EL (reprint author), Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Climate & Ecosyst Sci, Earth & Environm Sci, Berkeley, CA 94720 USA. EM njbouskill@lbl.gov; elbrodie@lbl.gov RI Bouskill, Nick/G-2390-2015; Brodie, Eoin/A-7853-2008; Nico, Peter/F-6997-2010; Holman, Hoi-Ying/N-8451-2014; Hao, Zhao/G-2391-2015; Gilbert, Benjamin/E-3182-2010; OI Brodie, Eoin/0000-0002-8453-8435; Nico, Peter/0000-0002-4180-9397; Holman, Hoi-Ying/0000-0002-7534-2625; Hao, Zhao/0000-0003-0677-8529; Northen, Trent/0000-0001-8404-3259 FU Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory; U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research [DE-AC02-05CH11231]; U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]; NSF grant [EAR-08199072]; Luquillo Critical Zone Observatory [EAR-0722476]; USGS Luquillo WEBB program; NSF [DEB 0620910] FX This work was partially supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory. The Subsurface Biogeochemical Research program, the Next Generation Ecosystem Experiment (NGEE-Tropics), and ENIGMA- Ecosystems and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov). All Scientific Focus Area Programs at Lawrence Berkeley National Laboratory supported by the U.S. Department of Energy, Office of Science, Office of Biological & Environmental Research under contract number DE-AC02-05CH11231. This work was also partially funded by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research under contract number DE-AC02-05CH11231, and by NSF grant EAR-08199072 to WLS, the Luquillo Critical Zone Observatory (EAR-0722476) with additional support provided by the USGS Luquillo WEBB program, and grant DEB 0620910 from NSF to the Institute of Tropical Forest Ecosystem Studies, University of Puerto Rico, and to the International Institute of Tropical Forestry USDA Forest Service, as part of the Luquillo Long-Term Ecological Research Program. NR 114 TC 0 Z9 0 U1 17 U2 50 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAR 15 PY 2016 VL 7 AR 323 DI 10.3389/fmicb.2016.00323 PG 14 WC Microbiology SC Microbiology GA DG5KS UT WOS:000372117500003 PM 27014243 ER PT J AU Bollmann, SR Fang, YF Press, CM Tyler, BM Grunwald, NJ AF Bollmann, Stephanie R. Fang, Yufeng Press, Caroline M. Tyler, Brett M. Gruenwald, Niklaus J. TI Diverse Evolutionary Trajectories for Small RNA Biogenesis Genes in the Oomycete Genus Phytophthora SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE dicer; RDR; evolution; Phytophthora; small RNA; stramenopile ID DOUBLE-STRANDED-RNA; C-ELEGANS; PARAMECIUM-TETRAURELIA; ARABIDOPSIS-THALIANA; GENOME SEQUENCE; DICER-LIKE; INFESTANS; POLYMERASE; PROTEIN; INTERFERENCE AB Gene regulation by small RNA pathways is ubiquitous among eukaryotes, but little is known about small RNA pathways in the Stramenopile kingdom. Phytophthora a genus of filamentous oomycetes, contains many devastating plant pathogens, causing multibillion-dollar damage to crops, ornamental plants, and natural environments. The genomes of several oomycetes including Phytophthora species such as the soybean pathogen P. sojae, have been sequenced, allowing evolutionary analysis of small RNA-processing enzymes. This study examined the evolutionary origins of the oomycete small RNA-related genes Dicer-like (DCL), and RNA-dependent RNA polymerase (RDR) through broad phylogenetic analyses of the key domains. Two Dicer gene homologs, DCL1 and DCL2, and one RDR homolog were cloned and analyzed from P sojae. Gene expression analysis revealed only minor changes in transcript levels among different life stages. Oomycete DCL1 homologs clustered with animal and plant Dicer homologs in evolutionary trees, whereas oomycete DCL2 homologs clustered basally to the tree along with Drosha homologs. Phylogenetic analysis of the RDR homologs confirmed a previous study that suggested the last common eukaryote ancestor possessed three RDR homologs, which were selectively retained or lost in later lineages. Our analysis clarifies the position of some Unikont and Chromalveolate RDR lineages within the tree, including oomycete homologs. Finally, we analyzed alterations in the domain structure of oomycete Dicer and RDR homologs, specifically focusing on the proposed domain transfer of the DEAD-box helicase domain from Dicer to RDR. Implications of the oomycete domain structure are discussed, and possible roles of the two oomycete Dicer homologs are proposed. C1 [Bollmann, Stephanie R.; Press, Caroline M.; Gruenwald, Niklaus J.] USDA ARS, Hort Crop Res Unit, Corvallis, OR USA. [Fang, Yufeng; Tyler, Brett M.; Gruenwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Fang, Yufeng; Tyler, Brett M.; Gruenwald, Niklaus J.] Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. [Fang, Yufeng] Virginia Tech, Interdisciplinary PhD Proaram Genet Bioinformat &, Blacksburg, VA USA. [Bollmann, Stephanie R.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crop Res Unit, Corvallis, OR USA.; Grunwald, NJ (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Grunwald, NJ (reprint author), Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. EM nik.grunwald@ars.usda.gov OI Grunwald, Niklaus/0000-0003-1656-7602 FU USDA National Research Initiative [2008-35600-18780]; USDA Agricultural Research Service (CRIS) [5358-22000-03900D] FX This work was supported by the USDA National Research Initiative (grant number 2008-35600-18780); and the USDA Agricultural Research Service (CRIS number 5358-22000-03900D). NR 81 TC 1 Z9 1 U1 2 U2 17 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 15 PY 2016 VL 7 AR 284 DI 10.3389/fpls.2016.00284 PG 15 WC Plant Sciences SC Plant Sciences GA DG3GS UT WOS:000371958100001 PM 27014308 ER PT J AU Zhang, LW Zhou, ZF Guo, QN Fokkens, L Miskei, M Pocsi, I Zhang, W Chen, M Wang, L Sun, YM Donzelli, BGG Gibson, DM Nelson, DR Luo, JG Rep, M Liu, H Yang, SN Wang, J Krasnoff, SB Xu, YQ Molnar, I Lin, M AF Zhang, Liwen Zhou, Zhengfu Guo, Qiannan Fokkens, Like Miskei, Marton Pocsi, Istvan Zhang, Wei Chen, Ming Wang, Lei Sun, Yamin Donzelli, Bruno G. G. Gibson, Donna M. Nelson, David R. Luo, Jian-Guang Rep, Martijn Liu, Hang Yang, Shengnan Wang, Jing Krasnoff, Stuart B. Xu, Yuquan Molnar, Istvan Lin, Min TI Insights into Adaptations to a Near-Obligate Nematode Endoparasitic Lifestyle from the Finished Genome of Drechmeria coniospora SO SCIENTIFIC REPORTS LA English DT Article ID FUNGUS MERIA-CONIOSPORA; PLANT-PARASITIC NEMATODES; CAENORHABDITIS-ELEGANS; ASPERGILLUS-NIDULANS; IRON HOMEOSTASIS; INNATE IMMUNITY; GENE FAMILY; C. ELEGANS; CONIDIA; INFECTION AB Nematophagous fungi employ three distinct predatory strategies: nematode trapping, parasitism of females and eggs, and endoparasitism. While endoparasites play key roles in controlling nematode populations in nature, their application for integrated pest management is hindered by the limited understanding of their biology. We present a comparative analysis of a high quality finished genome assembly of Drechmeria coniospora, a model endoparasitic nematophagous fungus, integrated with a transcriptomic study. Adaptation of D. coniospora to its almost completely obligate endoparasitic lifestyle led to the simplification of many orthologous gene families involved in the saprophytic trophic mode, while maintaining orthologs of most known fungal pathogen-host interaction proteins, stress response circuits and putative effectors of the small secreted protein type. The need to adhere to and penetrate the host cuticle led to a selective radiation of surface proteins and hydrolytic enzymes. Although the endoparasite has a simplified secondary metabolome, it produces a novel peptaibiotic family that shows antibacterial, antifungal and nematicidal activities. Our analyses emphasize the basic malleability of the D. coniospora genome: loss of genes advantageous for the saprophytic lifestyle; modulation of elements that its cohort species utilize for entomopathogenesis; and expansion of protein families necessary for the nematode endoparasitic lifestyle. C1 [Zhang, Liwen; Guo, Qiannan; Zhang, Wei; Chen, Ming; Lin, Min] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China. [Zhou, Zhengfu; Liu, Hang; Yang, Shengnan; Wang, Jing; Xu, Yuquan] Minist Agr, Key Lab Agr Genom Beijing, Beijing, Peoples R China. [Fokkens, Like; Rep, Martijn] Univ Amsterdam, Mol Plant Pathol, Amsterdam, Netherlands. [Miskei, Marton; Pocsi, Istvan] Univ Debrecen, Fac Sci & Technol, Dept Biotechnol & Microbiol, Debrecen, Hungary. [Miskei, Marton] Univ Debrecen, Dept Biochem & Mol Biol, Debrecen, Hungary. [Wang, Lei; Sun, Yamin] Nankai Univ, Tianjin Key Lab Microbial Funct Genom, TEDA Sch Biol Sci & Biotechnol, Tianjin 300071, Peoples R China. [Donzelli, Bruno G. G.] Cornell Univ, Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Gibson, Donna M.; Krasnoff, Stuart B.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Nelson, David R.] Univ Tennessee, Hlth Sci Ctr, Dept Microbiol Immunol & Biochem, Memphis, TN USA. [Luo, Jian-Guang] China Pharmaceut Univ, Dept Nat Med Chem, State Key Lab Nat Med, Nanjing, Jiangsu, Peoples R China. [Molnar, Istvan] Univ Arizona, Sch Nat Resources & Environm, Nat Prod Ctr, Tucson, AZ USA. RP Lin, M (reprint author), Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China.; Xu, YQ (reprint author), Minist Agr, Key Lab Agr Genom Beijing, Beijing, Peoples R China.; Molnar, I (reprint author), Univ Arizona, Sch Nat Resources & Environm, Nat Prod Ctr, Tucson, AZ USA. EM xuyuquan@caas.cn; imolnar@email.arizona.edu; linmin57@vip.163.com FU National Basic Research Program of China [2015CB755700]; National Science Foundation [MCB-0948751]; Hungarian Scientific Research Fund [OTKA K100464]; Netherlands Genomics Initiative; Natural Science Foundation of China [31500079]; "948" Project of Ministry of Agriculture of China [2015-Z1] FX This work was supported by the National Basic Research Program of China (2015CB755700 to M.L. and Y.X.); the National Science Foundation (MCB-0948751 to I.M.); the Hungarian Scientific Research Fund (OTKA K100464 to I.P.); the Netherlands Genomics Initiative (to M.R.); Natural Science Foundation of China (31500079 to L.Z.) and the "948" Project of Ministry of Agriculture of China (2015-Z1 to Y.X.). NR 82 TC 1 Z9 1 U1 6 U2 18 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 15 PY 2016 VL 6 AR 23122 DI 10.1038/srep23122 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG4PI UT WOS:000372053800001 PM 26975455 ER PT J AU Bankovich, B Boughton, E Boughton, R Avery, ML Wisely, SM AF Bankovich, Brittany Boughton, Elizabeth Boughton, Raoul Avery, Michael L. Wisely, Samantha M. TI Plant community shifts caused by feral swine rooting devalue Florida rangeland SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Cow-calf; Economic loss; Ecosystem damage; Invasive species; Plant diversity ID SOUTHEASTERN UNITED-STATES; INVASIVE ALIEN PLANTS; PERMUTATION TESTS; NATIONAL-PARK; SUS-SCROFA; WILD-BOAR; MANAGEMENT; DISTURBANCE; VEGETATION; IMPACTS AB Invasive species threaten agriculture by changing agroecosystem structure and function, reducing habitat value, decreasing biodiversity and ecosystem services and increasing management costs. Grazing lands in south central Florida are a mosaic of sown pastures, native grasslands, wetlands and woodlands that provide a variety of ecosystem services. Disturbance of these pastures and native grasslands by invasive feral swine (Sus scrofa) can have negative consequences for both economic productivity and biodiversity. In this study, we show that the effect of rooting on plant diversity depends on ecosystem type and initial levels of plant species diversity. For example, in native grassland pasture, rooting was initially associated with declines in plant species richness, while in sown pastures, rooting was associated with more sustained increases in plant species richness. In both sown pastures and native grasslands, swine rooting altered plant community composition reducing agricultural productivity. Forage grasses were primarily associated with unrooted areas, whereas low quality forage species or nuisance species were found in rooted areas. We provide an example of monetary losses that cattle ranches can incur when feral swine are abundant on the landscape and control is minimal. Ranch managers and government agencies are encouraged to consider implementing more stringent feral swine management programs to minimize negative effects of feral swine rooting on ecological and economic value of grazing lands. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Bankovich, Brittany; Boughton, Raoul; Wisely, Samantha M.] Univ Florida, Dept Wildlife Ecol & Conservat, 110 Newins Ziegler Hall, Gainesville, FL 32611 USA. [Boughton, Elizabeth] MacArthur Agroecol Res Ctr, 300 Buck Isl Ranch Rd, Lake Placid, FL 33852 USA. [Avery, Michael L.] APHIS Wildlife Serv, USDA, Natl Wildlife Res Ctr, 2820 East Univ Ave, Gainesville, FL 32641 USA. RP Wisely, SM (reprint author), Univ Florida, Dept Wildlife Ecol & Conservat, 110 Newins Ziegler Hall, Gainesville, FL 32611 USA. EM brittanybanko1@ufl.edu; eboughton@archbold-station.org; rboughton@ufl.edu; michael.l.avery@aphis.usda.gov; wisely@ufl.edu FU United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center [12-7412-0896-CA]; University of Florida Institute of Food and Agricultural Sciences Department of Wildlife Ecology and Conservation; MacArthur Agro-ecology Research Center FX We thank our funding agencies, the United States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center (Cooperative Agreement Number No. 12-7412-0896-CA), the University of Florida Institute of Food and Agricultural Sciences Department of Wildlife Ecology and Conservation, and the MacArthur Agro-ecology Research Center. We thank everyone who helped with sampling and plant identification and statistical methods, especially ranch manager G. Lollis, T. Campbell, M. Milleson, M. Hallett, A. Satterlee, T. McVay, F. Hernandez, J. Maki, and E. Bruna. NR 45 TC 2 Z9 2 U1 17 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD MAR 15 PY 2016 VL 220 BP 45 EP 54 DI 10.1016/j.agee.2015.12.027 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DF2QW UT WOS:000371189900006 ER PT J AU Ruiz, TM Sotomayor-Ramirez, D Torres-Melendez, C Martinez-Rodriguez, GA AF Ruiz, Teodoro M. Sotomayor-Ramirez, David Torres-Melendez, Carlos Martinez-Rodriguez, Gustavo A. TI Phosphorus mass cycling and balance in dairy farms: Case studies in Puerto Rico SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Whole-farm P balance; Internal P cycling; Soil-test P; Water quality; Dairy cow P intake ID STOCKING RATE; MANURE; MANAGEMENT; PASTURE; RUNOFF; COWS; PERFORMANCE; EXCRETION; TROPICS; YIELD AB Intensively managed dairy farms in Puerto Rico are suspected of being important contributors of phosphorus (P) in runoff that can reach surface-waters. A whole-farm P balance is a practical tool that can be used to estimate the net amounts of P retained within farms. In addition, knowledge of the internal mass-P cycling within farms (cows, pasture-lagoon, soils and forage) can be used to assess the relative risk of the on-farm agricultural practices to the environment and to identify management practices that reduce P accumulation in soils. Two dairy farms representative of feed and management practices in the Caribbean were assessed to describe internal mass-P cycling and to produce a whole-farm P balance. Farm A with an area of 58 ha had 120 lactating cows that rotationally grazed on improved tropical-grass pastures, and Farm B with an area of 310 ha had 482 lactating cows that were managed in semi confinement, were fed silage and concentrate during the day, and were grazed only at night. Over 60% (DM basis) of all feeds consumed by dairy cows were imported to the farms, accounting for 71% and 81% of the P consumed in Farms A and B, respectively. The majority of the total P consumed by lactating cows (65% in Farm A and 75% in Farm B) was from commercial dairy grain concentrate, and the remaining P inputs were from grazed and cut pasture. Phosphorus in milk and animals sold (outputs) was 21% of total P inputs in Farm A, and 17.5% in Farm B. Dietary P concentrations, in both farms, exceeded by more than 60% nutritional recommendations of P. On an annual basis, there was an annual surplus (i.e., positive P balance) of 3,132 kg P farm(-1) (54 kg P ha(-1)) and 18,290 kg P farm(-1) (59 kg P ha(-1)) in Farms A and B, respectively. At current milk production levels the practices with the greatest impact potential for reducing soil P enrichment in dairy farms are reducing P inputs in feeds, improving P recycling by maximizing harvested pasture, and reducing or discontinuing fertilizing P additions to grazed pastures. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ruiz, Teodoro M.] Univ Puerto Rico, Coll Agr Sci, Dept Anim Sci, Mayaguez Campus, Mayaguez, PR 00680 USA. [Sotomayor-Ramirez, David] Univ Puerto Rico, Coll Agr Sci, Dept Agroenvironm Sci, Mayaguez Campus, Mayaguez, PR 00680 USA. [Torres-Melendez, Carlos] Nat Resources Conservat Serv, USDA, Sebring Serv Ctr, Sebring, FL 33875 USA. [Martinez-Rodriguez, Gustavo A.] Univ Puerto Rico, Coll Agr Sci, Agr Expt Stn, Dept Agroenvironm Sci, Mayaguez Campus, Mayaguez, PR 00680 USA. RP Sotomayor-Ramirez, D (reprint author), Dept Agroenvironm Sci, POB 9000, Mayaguez, PR 00680 USA. EM david.sotomayor@upr.edu OI Torres-Melendez, Carlos/0000-0002-4072-5589 FU University of Puerto Rico, Mayaguez Agricultural Experiment Station; US Department of Agriculture FX This work was supported by a joint research program funded by the University of Puerto Rico, Mayaguez Agricultural Experiment Station and US Department of Agriculture (Hatch funds). NR 50 TC 0 Z9 0 U1 6 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD MAR 15 PY 2016 VL 220 BP 115 EP 124 DI 10.1016/j.agee.2016.01.003 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DF2QW UT WOS:000371189900013 ER PT J AU Waldo, S Chi, JS Pressley, SN O'Keeffe, P Pan, WL Brooks, ES Huggins, DR Stockle, CO Lamb, BK AF Waldo, Sarah Chi, Jinshu Pressley, Shelley N. O'Keeffe, Patrick Pan, William L. Brooks, Erin S. Huggins, David R. Stoeckle, Claudio O. Lamb, Brian K. TI Assessing carbon dynamics at high and low rainfall agricultural sites in the inland Pacific Northwest US using the eddy covariance method SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Eddy covariance; Cropland; Net ecosystem exchange; Net ecosystem carbon balance; Carbon balance; Rainfall zones ID FLUX-TOWER MEASUREMENTS; LIGHT-RESPONSE PARAMETERS; GROSS PRIMARY PRODUCTION; NET ECOSYSTEM EXCHANGE; LAND-USE CHANGE; DIOXIDE EXCHANGE; UNITED-STATES; NO-TILL; EASTERN WASHINGTON; CROPPING SYSTEMS AB Agricultural soils have the potential to be an important carbon (C) sink with proper management. The main goal of this study was to characterize C dynamics and net C exchange over two full crop years at two sites in the inland Pacific Northwest (iPNW). The iPNW is a highly productive dryland wheat growing region. The two measurement sites represent the low- and high-end of the regional precipitation gradient (250 and 550 mm year(-1), respectively). The low rainfall site is in a winter wheat-fallow rotation, while the high rainfall site is under continuous cropping with a rotation that includes winter wheat and pulse crops. The net ecosystem exchange of CO2 (NEE) was monitored using the eddy covariance method. The winter wheat cropping years were strong CO2 sinks, with net uptake of 517 +/- 26 and 524 +/- 29 g Cm-2 year(-1) at the high- and low-rainfall sites, respectively. The fallow and pulse-crop years were close to neutral with respect to NEE, with a net uptake of 20 +/- 38 g Cm-2 year(-1) for garbanzo beans at the high rainfall site, and a net loss of 3 +/- 19 g Cm-2 year(-1) for the fallow season at the low rainfall site. Combining NEE with other carbon exchange terms, most importantly carbon import and export via seeding and harvest, gives the net ecosystem carbon balance (NECB). We found that even when harvest export was taken into account, both sites acted as net carbon sinks over the two year period: the NECB at the continuous cropping site was 202 +/- 60 g Cm-2, and the crop-fallow site had a NECB of 444 +/- 34 g Cm-2. These results present useful insights to field-scale C dynamics on finely resolved timescales. To understand the potential of soils as a long term C sink however, longer-term monitoring over multiple complete crop rotation cycles in combination with other field measurements or process-based models will be necessary. (C) 2015 The Authors. Published by Elsevier B.V. C1 [Waldo, Sarah; Chi, Jinshu; Pressley, Shelley N.; O'Keeffe, Patrick; Lamb, Brian K.] Washington State Univ, Dept Civil & Environm Engn, Lab Atmospher Res, Pullman, WA 99164 USA. [Pan, William L.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Brooks, Erin S.] Univ Idaho, Dept Biol & Agr Engn, Moscow, ID 83844 USA. [Huggins, David R.] Washington State Univ, USDA ARS, Land Management & Water Conservat Res Unit, Pullman, WA 99164 USA. [Stoeckle, Claudio O.] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. RP Waldo, S (reprint author), Washington State Univ, Dept Civil & Environm Engn, Lab Atmospher Res, Pullman, WA 99164 USA. EM sarah.waldo@wsu.edu FU "Regional Approaches to Climate Change for Pacific Northwest Agriculture" (REACCH-PNA) project, USDA NIFA award [2011-68002-30191]; NSF IGERT "Nitrogen Systems: Policy-oriented Integrated Research and Education"; U.S. Environmental Protection Agency (EPA) [FP-91770001-0]; EPA STAR fellowship FX This study was funded through the "Regional Approaches to Climate Change for Pacific Northwest Agriculture" (REACCH-PNA) project, USDA NIFA award #2011-68002-30191. The assistance of Dave Uberuaga, Lauren Port, Heather Baxter, Laurel Strom, Jordan Strickland, and others at the Cook Agronomy Farm is gratefully acknowledged. We would also like to acknowledge the cooperation of the LIND grower, Rod Melcher. We would like to thank the two anonymous reviewers for their valuable insights which greatly improved the manuscript. SRW was also supported through the NSF IGERT "Nitrogen Systems: Policy-oriented Integrated Research and Education", and through the EPA STAR fellowship. This publication was developed under STAR Fellowship Assistance Agreement no. FP-91770001-0 awarded by the U.S. Environmental Protection Agency (EPA). It has not been formally reviewed by EPA. The views expressed in this publication are solely those of the authors, and EPA does not endorse any products or commercial services mentioned in this publication. NR 78 TC 2 Z9 2 U1 6 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2016 VL 218 BP 25 EP 36 DI 10.1016/j.agrformet.2015.11.018 PG 12 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DE8RT UT WOS:000370905100003 ER PT J AU Chi, JS Waldo, S Pressley, S O'Keeffe, P Huggins, D Stockle, C Pan, WL Brooks, E Lamb, B AF Chi, Jinshu Waldo, Sarah Pressley, Shelley O'Keeffe, Patrick Huggins, David Stoeckle, Claudio Pan, William L. Brooks, Erin Lamb, Brian TI Assessing carbon and water dynamics of no-till and conventional tillage cropping systems in the inland Pacific Northwest US using the eddy covariance method SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Eddy covariance; Cropland; Tillage practices; Net ecosystem exchange (NEE); Evapotranspiration (ET); Carbon and water budgets ID GROSS PRIMARY PRODUCTION; ENERGY-BALANCE CLOSURE; NET ECOSYSTEM EXCHANGE; WINTER-WHEAT FIELD; DIOXIDE EXCHANGE; SOIL RESPIRATION; TOWER MEASUREMENTS; CHIHUAHUAN DESERT; REDUCED TILLAGE; SOUTHERN BRAZIL AB Analysis of carbon and water budgets in cropping systems is important for understanding the impacts of different management practices and meteorological conditions, in the context of climate change, on agriculture. We have established a pair of long-term eddy covariance flux towers at the R. J. Cook Agronomy Farm (CAF) near Pullman, Washington, US. The tower sites have similar crop rotation, weather conditions, and management practices except tillage. One site has been under no-till management (CAF-NT) since 1998, while the other site has used conventional tillage practice (CAF-CT) over the same time period. Measurements conducted above a garbanzo bean crop (Cicer arietinum) between October 2012 and September 2013 showed the CAF-NT site was close to CO2 neutral with an annual cumulative net ecosystem exchange of CO2 (NEE) of -20 +/- 38 g Cm-2. By contrast, the CAF-CT site was a CO2 source with an annual NEE of 117 +/- 39 g Cm-2 during the same time period. The annual NEE values at each site were significantly different (p <0.05). When carbon losses via harvest export were taken into account, the net rates of carbon loss from each ecosystem to the atmosphere were 32 +/- 50 and 178 +/- 48 g Cm-2 for CAF-NT and CAF-CT, respectively, indicating CAF-NT was close to carbon neutral and CAF-CT was a net carbon source during the measurement period. Partitioning of NEE into gross primary productivity (GPP) and total ecosystem respiration (R-eco) shows that the annual cumulative GPP of each site did not differ much, but CAF-NT had lower annual cumulative R-eco compared to CAF-CT during the period of October 2012-September 2013. Water budget analyses presented similar magnitudes of annual sums of evapotranspiration (ET), 425 and 416 mm for CAF-NT and CAF-CT, respectively. ET was partitioned into evaporation (E) and transpiration (7) for both sites, and CAF-CT had a larger fraction of annual E compared to CAF-NT during the measurement period. Relationships between carbon and water fluxes were investigated, and for the period of October 2012-September 2013, high correlations were found in GPP vs. T and NEE vs. ET at both sites. In summary, the site deploying no-till practices had lower total ecosystem respiration and evaporation throughout the measurement period, more net carbon uptake and a greater ratio of transpiration over evapotranspiration (T/ET) during the growing season. (C) 2015 The Authors. Published by Elsevier B.V. C1 [Chi, Jinshu; Waldo, Sarah; Pressley, Shelley; O'Keeffe, Patrick; Lamb, Brian] Washington State Univ, Dept Civil & Environm Engn, Lab Atmospher Res, Pullman, WA 99164 USA. [Huggins, David] Washington State Univ, USDA ARS, Land Management & Water Conservat Res Unit, Pullman, WA 99164 USA. [Stoeckle, Claudio] Washington State Univ, Dept Biol Syst Engn, Pullman, WA 99164 USA. [Pan, William L.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Brooks, Erin] Univ Idaho, Dept Biol & Agr Engn, Moscow, ID 83844 USA. RP Chi, JS (reprint author), Washington State Univ, Dept Civil & Environm Engn, Lab Atmospher Res, Pullman, WA 99164 USA. EM jinshu.chi@wsu.edu FU National Institute for Food and Agriculture [2011-68002-30191]; China Scholarship Council FX This research was supported by the project of Regional Approaches to Climate Change for Pacific Northwest Agriculture (REACCH-PNA) which is funded through award#2011-68002-30191 from theNational Institute for Food and Agriculture. Additionally, funding for Jinshu Chi is being provided by theChina Scholarship Council. We would also like to acknowledge the rest of the REACCH objective 2 team members in particular Dave Uberuaga, Lauren Port, Margaret Davies, Laurel Strom, Heather Baxter, and Jordan Strickland for their help with the field work and biomass sample analysis, and Sansi Yang and Tsengel Nergui for their helpful suggestions on statistical analysis. We would like to thank the two anonymous reviewers for their valuable insights which greatly improved the manuscript. NR 90 TC 5 Z9 5 U1 8 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2016 VL 218 BP 37 EP 49 DI 10.1016/j.agrformet.2015.11.019 PG 13 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DE8RT UT WOS:000370905100004 ER PT J AU Li, X Xiao, QF Niu, JZ Dymond, S van Doorn, NS Yu, XX Xie, BY Lv, XZ Zhang, KB Li, J AF Li, Xiang Xiao, Qingfu Niu, Jianzhi Dymond, Salli van Doorn, Natalie S. Yu, Xinxiao Xie, Baoyuan Lv, Xizhi Zhang, Kebin Li, Jiao TI Process-based rainfall interception by small trees in Northern China: The effect of rainfall traits and crown structure characteristics SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Rainfall interception storage; Post-rainfall drainage; Rainfall intensity; Leaf area; Branch density ID 2-LAYER STOCHASTIC-MODEL; CANOPY STORAGE CAPACITY; DROP SIZE; LEAF-AREA; SOUTHEASTERN AUSTRALIA; SPATIAL VARIABILITY; TEMPORAL STABILITY; EUCALYPT FOREST; PINE PLANTATION; WATER STORAGE AB Rainfall interception by a tree's crown is one of the most important hydrological processes in an ecosystem, yet the mechanisms of interception are not well understood. A process-based experiment was conducted under five simulated rainfall intensities (from 10 to 150 mm h(-1)) to directly quantify tree crown interception and examine the effect of rainfall traits and crown structure characteristics on interception for broadleaf (Platycladus orientalis, Pinus tabulaeformis) and needle tree species (Quercus variabilis, Acer truncatum). Results indicated that (1) the interception process was composed of three phases, a rapid increase phase which accounted for approximately 90% of the maximum interception storage (C-max), a relatively-stable phase, and a post-rainfall drainage phase in which 40% (+/- 16%) of C-max drained off to reach the minimum interception storage (C-min); (2) C-max and C-min were only correlated with rainfall intensity for P. tabulaeformis; (3) C-max and C-min were correlated with both leaf traits (i.e., leaf area, leaf biomass, leaf morphology) and branch traits (i.e., branch density, branch count, branch length, woody surface area, and woody biomass), and the best predictors of C-max and C-min were biomass-related parameters; and (4) The needle species P. orientalis had the greatest C-max, while the largest C-min was observed in the broadleaf species A. truncatum. Our findings demonstrate the complexity of the interception process and tree characteristics may be more important in controlling interception than rainfall characteristics. (C) 2015 Elsevier B.V. All rights reserved. C1 [Li, Xiang; Niu, Jianzhi; Yu, Xinxiao; Xie, Baoyuan; Zhang, Kebin; Li, Jiao] Beijing Forestry Univ, Educ Minist, Key Lab Soil & Water Conservat & Desertificat Com, Beijing 100083, Peoples R China. [Xiao, Qingfu] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Dymond, Salli] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [van Doorn, Natalie S.] US Forest Serv, USDA, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Lv, Xizhi] Minist Water Resources, Yellow River Inst Hydraul Res, Key Lab Loess Plateau Soil Eros & Water Loss Proc, Zhengzhou 450003, Henan, Peoples R China. RP Niu, JZ (reprint author), Beijing Forestry Univ, Educ Minist, Key Lab Soil & Water Conservat & Desertificat Com, Beijing 100083, Peoples R China. EM nexk@bjfu.edu.cn FU Chinese Scholarship Council Fund; Fundamental Research Funds for the Central Universities [TD2011-03, BLYJ201406]; National Natural Science Foundation of China [41171028]; Central Nonprofit Research Institutions Basic Scientific Research Special Fund [HKY-JBYW-2016-04]; Key Laboratory of the Loess Plateau Soil Erosion and Water Loss Process and Control of Ministry of Water Resources Fund [2015004] FX We thank the Jiu Feng National Forest Park Administration, postgraduates Jun Xu, Chen Meng, and Pengwei Bao for their help and support in the experiment. We also thank the two anonymous reviewers for their thoughtful recommendations which have greatly improved the manuscript. This study was supported by the Chinese Scholarship Council Fund; Fundamental Research Funds for the Central Universities (no. TD2011-03, no. BLYJ201406); National Natural Science Foundation of China (41171028); Central Nonprofit Research Institutions Basic Scientific Research Special Fund (HKY-JBYW-2016-04); Key Laboratory of the Loess Plateau Soil Erosion and Water Loss Process and Control of Ministry of Water Resources Fund (2015004). None of the funders had any involvement in study design, data collection and analysis, preparation of the manuscript, nor the decision to submit and publish. NR 62 TC 1 Z9 2 U1 7 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2016 VL 218 BP 65 EP 73 DI 10.1016/j.agrformet.2015.11.017 PG 9 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DE8RT UT WOS:000370905100006 ER PT J AU Xie, J Chen, JQ Sun, G Zha, TS Yang, B Chu, HS Liu, JG Wan, SQ Zhou, CX Ma, H Bourque, CPA Shao, CL John, R Ouyang, ZT AF Xie, Jing Chen, Jiquan Sun, Ge Zha, Tianshan Yang, Bai Chu, Housen Liu, Junguo Wan, Shiqiang Zhou, Caixian Ma, Hong Bourque, Charles P. -A. Shao, Changliang John, Ranjeet Ouyang, Zutao TI Ten-year variability in ecosystem water use efficiency in an oak-dominated temperate forest under a warming climate SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Dryness index; Eddy covariance; Evapotranspiration; Gross ecosystem production; Long-term variability; Water use efficiency ID CARBON-DIOXIDE; EDDY COVARIANCE; TERRESTRIAL ECOSYSTEMS; ENVIRONMENTAL CONTROLS; SOIL RESPIRATION; SONIC ANEMOMETER; ASPEN FOREST; LONG-TERM; FLUXES; EXCHANGE AB The impacts of extreme weather events on water-carbon (C) coupling and ecosystem-scale water use efficiency (WUE) over a long term are poorly understood. We analyzed the changes in ecosystem water use efficiency (WUE) from 10 years of eddy-covariance measurements (2004-2013) over an oak-dominated temperate forest in Ohio, USA. The aim was to investigate the long-term response of ecosystem WUE to measured changes in site-biophysical conditions and ecosystem attributes. The oak forest produced new plant biomass of 2.5 +/- 0.2 gC kg(-1) of water loss annually. Monthly evapotranspiration (ET) and gross ecosystem production (GEP) were tightly coupled over the 10-year study period (R-2=0.94). Daily WUE had a linear relationship with air temperature (T-a) in low-temperature months and a unimodal relationship with T-a in high-temperature months during the growing season. On average, daily WUE ceased to increase when T-a exceeded 22 degrees C in warm months for both wet and dry years. Monthly WUE had a strong positive linear relationship with leaf area index (LAI), net radiation (R-n), and T-a and weak logarithmic relationship with water vapor pressure deficit (VPD) and precipitation (P) on a growing-season basis. When exploring the regulatory mechanisms on WUE within each season, spring LAI and P, summer R-n and T-a, and autumnal VPD and R-n were found to be the main explanatory variables for seasonal variation in WUE. The model developed in this study was able to capture 78% of growing-season variation in WUE on a monthly basis. The negative correlation between WUE and A in spring was mainly due to the high precipitation amounts in spring, decreasing GEP and WUE when LAI was still small, adding ET being observed to increase with high levels of evaporation as a result of high SWC in spring. Summer WUE had a significant decreasing trend across the 10 years mainly due to the combined effect of seasonal drought and increasing potential and available energy increasing ET, but decreasing GEP in summer. We concluded that seasonal dynamics of the interchange between precipitation and drought status of the system was an important variable in controlling seasonal WUE in wet years. In contrast, despite the negative impacts of unfavorable warming, available groundwater and an early start of the growing season were important contributing variables in high seasonal GEP, and thus, high seasonal WUE in dry years. (C) 2015 Elsevier B.V. All rights reserved. C1 [Xie, Jing; Zha, Tianshan] Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing 100083, Peoples R China. [Chen, Jiquan; Shao, Changliang; John, Ranjeet; Ouyang, Zutao] Michigan State Univ, CGCEO Geog, E Lansing, MI 48823 USA. [Xie, Jing; Zhou, Caixian; Ma, Hong] Beijing Forestry Carbon Sequestrat Adm, Beijing 100013, Peoples R China. [Sun, Ge] US Forest Serv, USDA, Southern Res Stn, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. [Yang, Bai] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37931 USA. [Chu, Housen] Univ Calif Berkeley, Dept Environm Sci, Berkeley, CA 94720 USA. [Liu, Junguo] Beijing Forestry Univ, Coll Nat Conservat, Beijing 100083, Peoples R China. [Wan, Shiqiang] Henan Univ, Coll Life Sci, Key Lab Plant Stress Biol, Kaifeng 475004, Henan, Peoples R China. [Bourque, Charles P. -A.] Univ New Brunswick, Forestry & Environm Management, POB 4400, Fredericton, NB E3B 5A3, Canada. RP Xie, J (reprint author), Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing 100083, Peoples R China.; Chen, JQ (reprint author), Michigan State Univ, CGCEO Geog, E Lansing, MI 48823 USA.; Xie, J (reprint author), Beijing Forestry Carbon Sequestrat Adm, Beijing 100013, Peoples R China. EM xiejingbj@126.com; jqchen@msu.edu RI Wan, Shiqiang/B-5799-2009; Chu, Housen/Q-6517-2016; Chen, Jiquan/D-1955-2009 OI Chu, Housen/0000-0002-8131-4938; FU Eastern Forest Environmental Threat Assessment Center; Southern Research Station [03-CA-11330147-073, 04-CA-11330147-238]; Toledo Area Metropark; US-China Carbon Consortium (USCCC) FX This study was funded by Eastern Forest Environmental Threat Assessment Center, Southern Research Station (Cooperative Agreements 03-CA-11330147-073 and 04-CA-11330147-238), the Toledo Area Metropark, and the US-China Carbon Consortium (USCCC) that promotes collaborative research among interested institutions in the US and China. We are thankful to many members of the Landscape Ecology and Ecosystem Sciences (LEES) Lab at Michigan State University, including Gabriela Shirkey, for their thoughtful comments and language assistance. NR 50 TC 4 Z9 4 U1 15 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2016 VL 218 BP 209 EP 217 DI 10.1016/j.agrformet.2015.12.059 PG 9 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DE8RT UT WOS:000370905100020 ER PT J AU Otkin, JA Anderson, MC Hain, C Svoboda, M Johnson, D Mueller, R Tadesse, T Wardlow, B Brown, J AF Otkin, Jason A. Anderson, Martha C. Hain, Christopher Svoboda, Mark Johnson, David Mueller, Richard Tadesse, Tsegaye Wardlow, Brian Brown, Jesslyn TI Assessing the evolution of soil moisture and vegetation conditions during the 2012 United States flash drought SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Flash drought; Drought monitoring; Soil moisture; Evapotranspiration; Crop impacts; Agriculture; Satellite data ID EVAPORATIVE STRESS INDEX; MAIZE YIELD; US DROUGHT; MODEL; HEAT; INFORMATION; TEMPERATURE; INDICATORS; NLDAS-2; MONITOR AB This study examines the evolution of several model-based and satellite-derived drought metrics sensitive to soil moisture and vegetation conditions during the extreme flash drought event that impacted major agricultural areas across the central U.S. during 2012. Standardized anomalies from the remote sensing based Evaporative Stress Index (ESI) and Vegetation Drought Response Index (VegDRI) and soil moisture anomalies from. the North American Land Data Assimilation System (NLDAS) are compared to the United States Drought Monitor (USDM), surface meteorological conditions, and crop and soil moisture data compiled by the National Agricultural Statistics Service (NASS). Overall, the results show that rapid decreases in the ESI and NLDAS anomalies often preceded drought intensification in the USDM by up to 6 wk depending on the region. Decreases in the ESI tended to occur up to several weeks before deteriorations were observed in the crop condition datasets. The NLDAS soil moisture anomalies were similar to those depicted in the NASS soil moisture datasets; however, some differences were noted in how each model responded to the changing drought conditions. The VegDRI anomalies tracked the evolution of the USDM drought depiction in regions with slow drought development, but lagged the USDM and other drought indicators when conditions were changing rapidly. Comparison to the crop condition datasets revealed that soybean conditions were most similar to ESI anomalies computed over short time periods (2-4wk), whereas corn conditions were more closely related to longer-range (8-12 wk) ESI anomalies. Crop yield departures were consistent with the drought severity depicted by the ESI and to a lesser extent by the NLDAS and VegDRI datasets. (C) 2015 Elsevier B.V. All rights reserved. C1 [Otkin, Jason A.] Univ Wisconsin, Cooperat Inst Meteorol Satellite Studies, 1225 W Dayton St, Madison, WI 53706 USA. [Anderson, Martha C.] ARS, USDA, Hydrol & Remote Sensing Lab, Bldg 007,BARC West, Beltsville, MD 20705 USA. [Hain, Christopher] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, 5825 Univ Res Court,Suite 4001, College Pk, MD 20740 USA. [Svoboda, Mark; Tadesse, Tsegaye; Wardlow, Brian] Univ Nebraska, Sch Nat Resources, Natl Drought Mitigat Ctr, 819 Hardin Hall,3310 Holdrege St,POB 830988, Lincoln, NE 68583 USA. [Johnson, David; Mueller, Richard] Natl Agr Stat Serv, USDA, 1400 Independence Ave SW, Washington, DC 20250 USA. [Wardlow, Brian] Univ Nebraska, Ctr Adv Land Management Informat Technol, 3310 Holdredge St, Lincoln, NE 68583 USA. [Brown, Jesslyn] US Geol Survey, Earth Resources Observat & Sci Ctr, 47914 252nd St, Sioux Falls, SD 57198 USA. RP Otkin, JA (reprint author), Univ Wisconsin, Cooperat Inst Meteorol Satellite Studies, 1225 W Dayton St, Madison, WI 53706 USA. EM jason.otkin@ssec.wisc.edu RI Tadesse, Tsegaye/O-7792-2015; Otkin, Jason/D-1737-2012; Anderson, Martha/C-1720-2015; OI Tadesse, Tsegaye/0000-0002-4102-1137; Otkin, Jason/0000-0003-4034-7845; Anderson, Martha/0000-0003-0748-5525; Brown, Jesslyn/0000-0002-9976-1998 FU NOAA Climate Program Office's Sectoral Applications Research Program (SARP); Modeling, Analysis, Predictions, and Projections (MAPP) [NA130AR4310122, NA140AR4310226]; USGS Climate and Land Use Change program FX This work was supported by funds provided by the NOAA Climate Program Office's Sectoral Applications Research Program (SARP) and the Modeling, Analysis, Predictions, and Projections (MAPP) program under grants NA130AR4310122 and NA140AR4310226. J. Brown was supported by the USGS Climate and Land Use Change program. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Comments from three anonymous reviewers improved the manuscript. NR 53 TC 7 Z9 7 U1 23 U2 52 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2016 VL 218 BP 230 EP 242 DI 10.1016/j.agrformet.2015.12.065 PG 13 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DE8RT UT WOS:000370905100022 ER PT J AU Kool, D Kustas, WP Ben-Gal, A Lazarovitch, N Heitman, JL Sauer, TJ Agam, N AF Kool, D. Kustas, W. P. Ben-Gal, A. Lazarovitch, N. Heitman, J. L. Sauer, T. J. Agam, N. TI Energy and evapotranspiration partitioning in a desert vineyard SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Evaporation; Transpiration; Latent heat; Sparse canopy; Water use efficiency; Energy balance; Water balance; Crop coefficient ID MODELING RADIATIVE BALANCE; LATENT-HEAT FLUX; SOIL SURFACE; WATER-USE; ROW-CROP; MICROLYSIMETRY TECHNIQUES; CANOPY EVAPORATION; IRRIGATED VINEYARD; SONIC ANEMOMETER; NET-RADIATION AB The challenge of partitioning energy and evapotranspiration (ET) components was addressed over a season (bud break till harvest) in a wine grape vineyard located in an extreme arid region. A below canopy energy balance approach was applied to continuously estimate evaporation from the soil (E) while system ET was measured using eddy covariance. Below canopy energy balance was assessed at the dry midrow position as well as the wet irrigated position directly underneath the vine row, with E calculated as the residual of measured net radiation, soil heat flux, and computed sensible heat flux. The variables used to compute sensible heat flux included soil surface temperature measured using infrared thermometers and below-canopy wind speed in a soil resistance formulation that required a modified wind factor. The E derived from below canopy energy balance was reasonable at daily intervals although it underestimated micro-lysimeter E measurements, suggesting there may have been advected energy from the midrow to the below-vine position. Seasonal partitioning indicated that total E amounted to 9-11% of ET. In addition, empirical functions from the literature relating crop coefficients (K-cb) to plant size, appeared to give reasonable results under full canopy, albeit with some day to day variation, but underestimated K-cb during the growing period. (C) 2016 Elsevier B.V. All rights reserved. C1 [Kool, D.; Lazarovitch, N.; Agam, N.] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Sede Boger Campus, IL-84990 Beer Sheva, Israel. [Kool, D.; Ben-Gal, A.] Agr Res Org, Inst Soil Water & Environm Sci, Gilat Res Ctr, Mobile Post Negev 2, IL-85280 Gilat, Israel. [Kustas, W. P.] ARS, USDA, Hydrol & Remote Sensing Lab, Bldg 007,Rm 104, Beltsville, MD 20705 USA. [Heitman, J. L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Sauer, T. J.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Agam, N (reprint author), Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Sede Boger Campus, IL-84990 Beer Sheva, Israel. EM agam@bgu.ac.il RI Kool, Dilia/F-8273-2015; Agam, Nurit/E-1836-2013 OI Kool, Dilia/0000-0002-8332-1429; Agam, Nurit/0000-0002-8921-6179 FU BARD [US-4262-09]; United States Israel Binational Agricultural Research and Development Fund; I-CORE Program of the Planning and Budgeting Committee and the Israel Science Foundation [152/11] FX This research was supported by Research Grant No. US-4262-09 from BARD, the United States Israel Binational Agricultural Research and Development Fund, and was partially supported by the I-CORE Program of the Planning and Budgeting Committee and the Israel Science Foundation (grant no. 152/11). We are particularly grateful for technical and field support from the Gilat Research Center's Eugene Presnov and the Jacob Blaustein Institutes for Desert Research's Yuval Shani. We also thank Yoav Rabani for graciously providing his vineyard as an experimental site. NR 61 TC 1 Z9 1 U1 11 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAR 15 PY 2016 VL 218 BP 277 EP 287 DI 10.1016/j.agrformet.2016.01.002 PG 11 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DE8RT UT WOS:000370905100027 ER PT J AU Puhlick, JJ Fraver, S Fernandez, IJ Weiskittel, AR Kenefic, LS Kolka, RK Gruselle, MC AF Puhlick, Joshua J. Fraver, Shawn Fernandez, Ivan J. Weiskittel, Aaron R. Kenefic, Laura S. Kolka, Randall K. Gruselle, Marie-Cecile TI Factors influencing organic-horizon carbon pools in mixed-species stands of central Maine, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Silviculture; Forest soil; Forest floor; Carbon storage; Bryophytes; Pedotransfer functions ID SOUTHERN NEW-ENGLAND; SOIL DRAINAGE CLASS; LITTER DECOMPOSITION; BOREAL FORESTS; DEADWOOD ABUNDANCE; NORWAY SPRUCE; MINERAL SOIL; WOODY DEBRIS; PINE FORESTS; PICEA-ABIES AB The overall goal of this study was to evaluate the correlation of multiple abiotic and biotic factors with organic-horizon (O-horizon) carbon (C) content on the Penobscot Experimental Forest in central Maine, USA. O-horizon samples were collected and their associated depths were recorded from stands managed with a range of silvicultural and harvesting treatments (i.e., selection, shelterwood, and commercial clearcut) and an unmanaged control. The overall mean for O-horizon C content from all samples was 25.6 +/- 16.1 Mg ha(-1) (mean +/- SD). The samples were used to develop a pedotransfer function for predicting O-horizon C content from O-horizon depth (R-2 = 0.47, RMSE = 1.6 Mg ha(-1)) so that an average of O-horizon C content could be calculated for permanent sample plots on which abiotic and biotic factors were quantified. O-horizon depth measurements recorded along transects on permanent sample plots were used to calculate plot average O-horizon C content. There were no significant differences in average predicted O-horizon C content among selection, shelterwood, and commercial clearcut treatments. However, variation in predicted O-horizon C content between stands where the same treatment was applied was statistically significant and was likely due to the timing of harvests and abundance of dead wood buried within O horizons. Depth to redoximorphic features, cartographic depth to water table or saturated zone, drainage class, fine woody debris mass, downed woody debris volume, tree basal area, and the relative basal area of conifer species were not significant predictors of predicted O-horizon C content at the plot level. When the individual predicted values of O-horizon C content were modeled, within plot variation accounted for 83.8% of the variance. Bryophyte mass, which was predicted from bryophyte cover, only explained 1.2% of the variation in O-horizon C content at the microsite level. These results highlight the sizeable variability in O-horizon C content within and among these mixed-species stands with various forest management and natural disturbance histories. (C) 2016 Elsevier B.V. All rights reserved. C1 [Puhlick, Joshua J.; Fraver, Shawn; Fernandez, Ivan J.; Weiskittel, Aaron R.; Gruselle, Marie-Cecile] Univ Maine, Sch Forest Resources, 5755 Nutting Hall, Orono, ME 04469 USA. [Kenefic, Laura S.] US Forest Serv, USDA, No Res Stn, 686 Govt Rd, Bradley, ME 04411 USA. [Kolka, Randall K.] US Forest Serv, USDA, No Res Stn, 1831 Hwy 169 E, Grand Rapids, MN 55744 USA. RP Puhlick, JJ (reprint author), Univ Maine, Sch Forest Resources, 5755 Nutting Hall, Orono, ME 04469 USA. EM joshua.puhlick@maine.edu; shawn.fraver@maine.edu; ivanjf@maine.edu; aaron.weiskittel@maine.edu; lkenefic@fs.fed.us; rkolka@fs.fed.us; mariececile.gruselle@maine.edu FU U.S. Forest Service, Northern Research Station; Northeastern States Research Cooperative; Maine Agricultural and Forest Experiment Station; Penobscot Experimental Forest Research Fund FX We thank Valerie Kurth (Flathead Conservation District) who provided useful comments that helped us to improve this manuscript. We also thank Robert Seymour (University of Maine) and John Brissette (U.S. Forest Service) for their discussions about the project. We acknowledge Lindsey Rustad (U.S. Forest Service) and Bruce Hoskins, Sue Erich, and Cheryl Spencer (University of Maine) for their advice on soil sampling and laboratory procedures. Funding for this project was provided by the U.S. Forest Service, Northern Research Station and Northeastern States Research Cooperative; the Maine Agricultural and Forest Experiment Station; and the Penobscot Experimental Forest Research Fund. NR 77 TC 4 Z9 3 U1 6 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 15 PY 2016 VL 364 BP 90 EP 100 DI 10.1016/j.foreco.2016.01.0069 PG 11 WC Forestry SC Forestry GA DE8LI UT WOS:000370887100011 ER PT J AU Swaim, JT Dey, DC Saunders, MR Weigel, DR Thornton, CD Kabrick, JM Jenkins, MA AF Swaim, J. Travis Dey, Daniel C. Saunders, Michael R. Weigel, Dale R. Thornton, Christopher D. Kabrick, John M. Jenkins, Michael A. TI Predicting the height growth of oak species (Quercus) reproduction over a 23-year period following clearcutting SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Competition; Long-term data; Stand development; Even-aged; Central hardwood region ID RANDOM FORESTS; ADVANCE REPRODUCTION; COMPETITIVE SUCCESS; VARIABLE IMPORTANCE; SOUTHERN INDIANA; REGENERATION; SEEDLINGS; DYNAMICS; SURVIVAL; CLASSIFICATION AB We resampled plots from a repeated measures study implemented on the Hoosier National Forest (HNF) in southern Indiana in 1988 to investigate the influence of site and seedling physical attributes on height growth and establishment success of oak species (Quercus spp.) reproduction in stands regenerated by the clearcut method. Before harvest, an array of physical attributes were documented for individual stems of advance reproduction. Across all surveys, the same characteristics were remeasured in years 6, 12, and 23 for all reproduction types (advance reproduction, stump sprouts, and new seedlings). In order to characterize topo-edaphic conditions, soil samples were collected and analyzed in 2011, and slope aspect, slope percent, and slope position were measured in the field. Random Forest (RF) analysis was used to determine the best physical and environmental predictors of height growth for oak species and their competitors in developing stands. Overall, advance reproduction of oak species fared poorly following harvests. Sprout-origin oak stems proved stronger competitors in developing stands, although their abundance relative to competing species was quite low. Advance and sprout origin maple (Acer spp.) stems, along with new seedlings of black cherry (Prunus serotina Ehrh.) and yellow-poplar (Liriodendron tulipifera L.), quickly overtopped oak advance reproduction and established dominance in the developing canopy. The height of stems during prior sampling periods was the best overall predictor of stem height in subsequent sampling periods. Species was also an important predictor of stem height. Comparatively, environmental variables were poor predictors of height growth of individual stems throughout the study, although more mesic aspects, greater cation exchange capacity, and greater soil magnesium saturation were associated with greater height of non-sprout origin stems from species groups other than oak or hickory in year 6. Our results suggest that overstory removal has driven stand demographics towards species favored by infrequent large-scale disturbance events such as clearcutting. Without post-harvest treatments to control competitors, oak regeneration on more mesic sites is unlikely to recruit into developing stands. (C) 2016 Elsevier B.V. All rights reserved. C1 [Swaim, J. Travis; Saunders, Michael R.; Jenkins, Michael A.] Purdue Univ, Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA. [Swaim, J. Travis; Saunders, Michael R.; Jenkins, Michael A.] Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. [Dey, Daniel C.; Weigel, Dale R.; Kabrick, John M.] US Forest Serv, USDA, No Res Stn, 202 ABNR Bldg, Columbia, MO 65211 USA. [Thornton, Christopher D.] Hoosier Natl Forest, 811 Constitut Ave, Bedford, IN 47421 USA. [Swaim, J. Travis] Malheur Natl Forest, 265 Highway 20 South, Hines, OR 97738 USA. RP Jenkins, MA (reprint author), Purdue Univ, Dept Forestry & Nat Resources, 715 West State St, W Lafayette, IN 47907 USA.; Jenkins, MA (reprint author), Purdue Univ, Hardwood Tree Improvement & Regenerat Ctr, 715 West State St, W Lafayette, IN 47907 USA. EM jenkinma@purdue.edu RI Jenkins, Michael/F-7396-2016 FU USDA Forest Service Northern Research Station; Hoosier National Forest under Joint Venture Agreement [11-JV-11242311-013] FX We thank Charlie Jackson, Lindsay Jenkins, Andy Meier, and Josh Shields for their assistance with fieldwork and analyses. We also thank the personnel of the Hoosier National Forest for their assistance and logistical support. Funding for this study was provided by the USDA Forest Service Northern Research Station and Hoosier National Forest under Joint Venture Agreement 11-JV-11242311-013. NR 59 TC 1 Z9 1 U1 11 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAR 15 PY 2016 VL 364 BP 101 EP 112 DI 10.1016/j.foreco.2016.01.005 PG 12 WC Forestry SC Forestry GA DE8LI UT WOS:000370887100012 ER PT J AU Uchimiya, M Liu, ZZ Sistani, K AF Uchimiya, Minori Liu, Zhongzhen Sistani, Karamat TI Field-scale fluorescence fingerprinting of biochar-borne dissolved organic carbon SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Pyrolysis; Biomass; Thermal conversion; Volatile matter; Soil amendment ID PARALLEL FACTOR-ANALYSIS; POLYCYCLIC AROMATIC-HYDROCARBONS; BLACK CARBON; SOIL; MATTER; SPECTROSCOPY; PYROLYSIS; CARBONIZATION; MECHANISM; SORPTION AB Biochar continues to receive worldwide enthusiasm as means of augmenting recalcitrant organic carbon in agricultural soils. Realistic biochar amendment rate (typically less than 1 wt%) in the field scale, and subsequent loss by sizing, rain, and other transport events demand reliable methods to quantify the remaining portions of amended biochar. This study employed fluorescence excitation-emission (EEM) spectrophotometry and parallel factor analysis (PARAFAC) to specifically target pyrogenic dissolved organic carbon (DOC) released by amended biochar during the course of a field trial at Bowling Green, KY experimental site. Toluene/methanol (1:6 v/v) extracts of surface (0-15 cm) soils amended with 21.28 t ha(-1) fast pyrolysis biochar afforded PARAFAC fingerprints representing different degrees of aromaticity. Compared to the control without treatments, biochar treatment (with and without poultry manure or chemical fertilizer) increased the relative contribution of PARAFAC fingerprint attributable to labile polyaromatic DOC structures. Poultry manure or chemical fertilizer alone (without biochar) did not influence the amounts of polyaromatic DOC structures. Existence of biochar could be further validated by the changes in %DOC (relative to the total carbon), fixed C content, and UV absorbance (360 nm), whereas FTIR, %O, and sorption of model agrochemical (deisopropylatrazine) did not reflect the presence of biochar in the soil samples. Developed toluene/methanol-based EEM-PARAFAC technique will provide a sensitive, rapid, and cost-competitive method to validate the long-term carbon sequestration by the biochar soil amendment. Published by Elsevier Ltd. C1 [Uchimiya, Minori; Liu, Zhongzhen] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Liu, Zhongzhen] Guangdong Acad Agr Sci, Inst Agr Resources & Environm, Guangzhou, Guangdong, Peoples R China. [Sistani, Karamat] USDA ARS, Food Anim Environm Syst Res Unit, 2413 Nashville Rd B-5, Bowling Green, KY 42101 USA. RP Uchimiya, M (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 29 TC 0 Z9 0 U1 20 U2 51 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD MAR 15 PY 2016 VL 169 BP 184 EP 190 DI 10.1016/j.jenvman.2015.12.009 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DE8TL UT WOS:000370909500020 PM 26751812 ER PT J AU Buotte, PC Peterson, DL McKelvey, KS Hicke, JA AF Buotte, Polly C. Peterson, David L. McKelvey, Kevin S. Hicke, Jeffrey A. TI Capturing subregional variability in regional-scale climate change vulnerability assessments of natural resources SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Climate change; Vulnerability assessments; Geographic variability; Natural resources ID WASHINGTON-STATE AB Natural resource vulnerability to climate change can depend on the climatology and ecological conditions at a particular site. Here we present a conceptual framework for incorporating spatial variability in natural resource vulnerability to climate change in a regional-scale assessment. The framework was implemented in the first regional-scale vulnerability assessment conducted by the US Forest Service. During this assessment, five subregional workshops were held to capture variability in vulnerability and to develop adaptation tactics. At each workshop, participants answered a questionnaire to: 1) identify species, resources, or other information missing from the regional assessment, and 2) describe subregional vulnerability to climate change. Workshop participants divided into six resource groups; here we focus on wildlife resources. Participants identified information missing from the regional assessment and multiple instances of subregional variability in climate change vulnerability. We provide recommendations for improving the process of capturing subregional variability in a regional vulnerability assessment. We propose a revised conceptual framework structured around pathways of climate influence, each with separate rankings for exposure, sensitivity, and adaptive capacity. These revisions allow for a quantitative ranking of species, pathways, exposure, sensitivity, and adaptive capacity across subregions. Rankings can be used to direct the development and implementation of future regional research and monitoring programs. The revised conceptual framework is equally applicable as a stand-alone model for assessing climate change vulnerability and as a nested model within a regional assessment for capturing subregional variability in vulnerability. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Buotte, Polly C.] Univ Idaho, Environm Sci Program, Moscow, ID 83844 USA. [Buotte, Polly C.; Hicke, Jeffrey A.] Univ Idaho, Dept Geog, 875 Perimeter Dr,MS 3021, Moscow, ID 83844 USA. [Peterson, David L.] US Forest Serv, Pacific NW Res Stn, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. [McKelvey, Kevin S.] US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. RP Buotte, PC (reprint author), Univ Idaho, Dept Geog, 875 Perimeter Dr,MS 3021, Moscow, ID 83844 USA. EM pbuotte@uidaho.edu; peterson@fs.fed.us; kmckelvey@fs.fed.us; jhicke@uidaho.edu FU graduate student fellowship in climate science at the University of Idaho from the U.S. Department of Interior Northwest Climate Science Center; USFS Pacific Northwest Research Station; Office of Sustainability and Climate Change FX We thank Linh Hoang and Jessica Halofsky of the NRAP leadership team for helpful discussions. P.C. Buotte was supported by a graduate student fellowship in climate science at the University of Idaho from the U.S. Department of Interior Northwest Climate Science Center. D. L. Peterson was supported by the USFS Pacific Northwest Research Station and Office of Sustainability and Climate Change. NR 26 TC 1 Z9 1 U1 3 U2 7 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD MAR 15 PY 2016 VL 169 BP 313 EP 318 DI 10.1016/j.jenvman.2015.12.017 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA DE8TL UT WOS:000370909500033 PM 26796918 ER PT J AU Casas, A Garcia, M Siegel, RB Koltunov, A Ramirez, C Ustin, S AF Casas, Angeles Garcia, Mariano Siegel, Rodney B. Koltunov, Alexander Ramirez, Carlos Ustin, Susan TI Burned forest characterization at single-tree level with airborne laser scanning for assessing wildlife habitat SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Gaussian processes; Watershed segmentation; Single-tree level; Stem diameter; Basal area; Megafire; Wildlife habitat; Black-backed woodpecker; Airborne laser scanning; LiDAR; Snags; Dead trees; Rim fire ID DISCRETE-RETURN LIDAR; AVIAN SPECIES RICHNESS; COARSE WOODY DEBRIS; WOODPECKER HABITAT; INDIVIDUAL TREES; SMALL-FOOTPRINT; BIRD; FIRE; INTENSITY; AREA AB Abundance, size, and spatial distribution of standing dead trees (snags), are key indicators of forest biodiversity and ecosystem health. These metrics represent critical habitat components for various wildlife species of conservation concern, including the Black-backed Woodpecker (Picoides arcticus), which is strongly associated with recently burned conifer forest. We assessed the potential of Airborne Laser Scanning (ALS) to detect and characterize conifer snags and identify Black-backed Woodpecker habitat using previously derived empirical thresholds of conifer snag basal area. Over the footprint of the Rim Fire, a megafire that extended (similar to 104,000 ha) through a heterogeneous mosaic of conifer forests, oak woodlands, and meadows in the Sierra Nevada mountains of California, we identified conifer snags and estimated their basal area from single-tree ALS-derived metrics using Gaussian processes in four major steps. First, individual trees were mapped using the Watershed Segmentation algorithm, resulting in 87% detection of trees with stem diameter larger than 30 cm. Second, the snag/live classification model identified snags with an overall accuracy of 91.8%, using the coefficient of variation of height and intensity together with maximum intensity and fractional cover as the most relevant metrics. Third, the conifer/hardwood snag classification model utilizing the maximum height, median height, minimum intensity, and area metrics separated snag forest types with an overall accuracy of 84.8%. Finally, a Gaussian process regression model reliably estimated conifer snag stem diameter (R-2 = 0.81) using height and crown area, thus significantly outperforming regionally calibrated conifer-specific allometric equations. As a result,-80% of the snag basal area have been mapped. Optimal and potential habitat for Black-backed Woodpecker comprise 53.7 km(2) and 58.4 km(2), respectively, representing 5.1 and 5.6% of the footprint of the Rim Fire. Our study illustrates the utility of high-density ALS data for characterizing recently burned forests, which, in conjunction with information about the habitat needs of particular snag-dependent wildlife species, can be used to assess habitat characteristics, and thus contribute greatly to forest management and biodiversity conservation. (c) 2016 Elsevier Inc. All rights reserved. C1 [Casas, Angeles; Koltunov, Alexander; Ustin, Susan] Univ Calif Davis, Dept Land Air & Water Resources, One Shields Ave, Davis, CA 95616 USA. [Garcia, Mariano] Univ Leicester, Ctr Landscape & Climate Res, Univ Rd, Leicester LE1 7RH, Leics, England. [Garcia, Mariano] CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. [Siegel, Rodney B.] Inst Bird Populat, Point Reyes Stn, CA USA. [Koltunov, Alexander; Ramirez, Carlos] US Forest Serv, USDA, Reg Remote Sensing Lab 5, Mcclellan, CA USA. RP Casas, A (reprint author), Univ Calif Davis, Dept Land Air & Water Resources, One Shields Ave, Davis, CA 95616 USA. FU USFS; Marie Curie IOF (ForeStMap-3D Forest Structure Monitoring and Mapping) [629376] FX This research was carried out within the ambit of the project Multi sensor remote sensing study of California's Rim Fire to inform post-fire ecosystem restoration and effective prevention of future catastrophic wildfires. Sponsor: USFS. Mariano Garcia is supported by the Marie Curie IOF (ForeStMap-3D Forest Structure Monitoring and Mapping, Project Reference: 629376). The contents on this paper reflect only the authors views and not the views of the European Commission. We greatly appreciate the help of Mitzi Thornley and the Science Division Yosemite National Park for the permission to conduct research in Yosemite National Park (YOSE-00653/YOSE-2014-SCI-0124). NR 76 TC 3 Z9 3 U1 7 U2 28 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD MAR 15 PY 2016 VL 175 BP 231 EP 241 DI 10.1016/j.rse.2015.12.044 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DF0AC UT WOS:000371000000020 ER PT J AU Adviento-Borbe, MAA Linquist, B AF Adviento-Borbe, Maria Arlene A. Linquist, Bruce TI Assessing fertilizer N placement on CH4 and N2O emissions in irrigated rice systems SO GEODERMA LA English DT Article DE CH4; N2O; Grain yield; Fertilizer N; Deep N placement; Rice ID NITROUS-OXIDE EMISSIONS; FLOODED RICE; METHANE EMISSIONS; FIELD MEASUREMENT; JAVA AB Improved N fertilizer management practices can increase rice yields and mitigate global warming potential (GWP). While banding N has been shown to have positive effects on yield and nitrogen use efficiency (NUE), there is little information on how it affects greenhouse gas (GHG) emissions from flooded rice systems. We tested the hypothesis that in continuously flooded rice systems where GWP is dominated by CH4 emissions, deep placement of urea in bands would reduce CH4 and N2O emissions. Rice yields and GHG emissions were measured from three field experiments which had three treatments: (1) no N (NO), (2) urea broadcast (U-BR) on soil surface and (3) urea banded at 7.5 cm soil depth (U-BA). All urea was applied in a single application before flooding in preparation for planting at N rates of 143-150 kg N ha(-1). Throughout the rice growing season GHG emissions were measured using a vented flux chamber and gas chromatograph. Across all fields, N fertilizer application increased yield on average by 121%. Between the N placement methods, grain yields and NUE (37 kg grain kg(-1)) were similar. Daily N2O emissions were low to negative and did not differ among treatments. CH4 emissions were the major source of GWP emissions and cumulative emissions ranged from 6.3 to 297 kg CH4-C ha(-1) season (-1) among fields. While in some cases fertilizer N increased CH4 emissions, there was no effect of N placement on them. Published by Elsevier B.V. C1 [Adviento-Borbe, Maria Arlene A.] ARS, Delta Water Management Res Unit, USDA, Jonesboro, AR 72401 USA. [Linquist, Bruce] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Adviento-Borbe, MAA (reprint author), ARS, Delta Water Management Res Unit, USDA, Jonesboro, AR 72401 USA. EM Arlene.AdvientoBorbe@ars.usda.gov; balinquist@ucdavis.edu FU California Rice Research Board FX We thank C. Abrenilla, C. Mikita, A. Hervani, K. Anderson, X. Liang, L. Liu, A Dennett, S. Hussain, and L Rosa for their help in the field. Also we thank Mr. C. Mathews for allowing us to conduct field trial in his farm and the California Rice Research Board for funding this research. NR 40 TC 2 Z9 3 U1 12 U2 69 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD MAR 15 PY 2016 VL 266 BP 40 EP 45 DI 10.1016/j.geoderma.2015.11.034 PG 6 WC Soil Science SC Agriculture GA DD1LB UT WOS:000369682100004 ER PT J AU Cabezon, O Cerda-Cuellar, M Morera, V Garcia-Bocanegra, I Gonzalez-Solis, J Napp, S Ribas, MP Blanch-Lazaro, B Fernandez-Aguilar, X Antilles, N Lopez-Soria, S Lorca-Oro, C Dubey, JP Almeria, S AF Cabezon, Oscar Cerda-Cuellar, Marta Morera, Virginia Garcia-Bocanegra, Ignacio Gonzalez-Solis, Jacob Napp, Sebastian Ribas, Maria P. Blanch-Lazaro, Berta Fernandez-Aguilar, Xavier Antilles, Noelia Lopez-Soria, Sergio Lorca-Oro, Cristina Dubey, Jitender P. Almeria, Sonia TI Toxoplasma gondii Infection in Seagull Chicks Is Related to the Consumption of Freshwater Food Resources SO PLOS ONE LA English DT Article ID NEOSPORA-CANINUM; ARCTIC FOXES; WILD BIRDS; SEROPREVALENCE; PREVALENCE; OOCYSTS; TRANSMISSION; IMMUNOLOGY; ANTIBODIES; SURVIVAL AB Understanding the spread of Toxoplasma gondii (T. gondii) in wild birds, particularly in those with opportunistic feeding behavior, is of interest for elucidating the epidemiological involvement of these birds in the maintenance and dissemination of the parasite. Overall, from 2009 to 2011, we collected sera from 525 seagull chicks (Yellow-legged gull (Larus michahellis) and Audouin's gull (L. audouinii)) from 6 breeding colonies in Spain and tested them using the modified agglutination test (MAT) for the presence of antibodies against T. gondii. Chick age was estimated from bill length. Main food source of seagull chicks was evaluated using stable isotope analyses from growing scapular feathers. Overall T. gondii seroprevalence was 21.0% (IC95% 17.5-24.4). A generalized linear mixed-effects model indicated that year (2009) and food source (freshwater) were risk factors associated to the individual risk of infection by T. gondii, while age (days) was close to significance. Freshwater food origin was related to the highest seroprevalence levels, followed by marine origin, supporting freshwater and sewages as important routes of dispersion of T. gondii. Year differences could indicate fluctuating rates of exposure of seagull chicks to T. gondii. Age ranged from 4 to 30 days and seropositivity tended to increase with age (P = 0.07), supporting that seropositivity is related to T. gondii infection rather than to maternal transfer of antibodies, which in gulls is known to sharply decrease with chick age. This study is the first to report T. gondii antibodies in Yellow-legged and Audouin's gulls, thereby extending the range of intermediate hosts for this parasite and underscoring the complexity of its epidemiology. C1 [Cabezon, Oscar; Ribas, Maria P.; Blanch-Lazaro, Berta; Fernandez-Aguilar, Xavier] Univ Autonoma Barcelona, Fac Vet, Dept Med & Cirurgia Anim, Serv Ecopatol Fauna Salvatge, Bellaterra, Spain. [Cabezon, Oscar; Cerda-Cuellar, Marta; Napp, Sebastian; Fernandez-Aguilar, Xavier; Antilles, Noelia; Lopez-Soria, Sergio; Lorca-Oro, Cristina; Almeria, Sonia] Ctr Recerca Sanitat Anim CReSA, IRTA, Campus Univ Autonoma Barcelona, Barcelona, Spain. [Morera, Virginia; Gonzalez-Solis, Jacob] Univ Barcelona, Inst Recerca Biodiversitat IRBio, Barcelona, Spain. [Morera, Virginia; Gonzalez-Solis, Jacob] Univ Barcelona, Dept Biol Anim, Barcelona, Spain. [Garcia-Bocanegra, Ignacio] UCO, Fac Vet, Dept Sanidad Anim, Cordoba, Spain. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. [Almeria, Sonia] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat & Anat Anim, Bellaterra, Spain. RP Cabezon, O (reprint author), Univ Autonoma Barcelona, Fac Vet, Dept Med & Cirurgia Anim, Serv Ecopatol Fauna Salvatge, Bellaterra, Spain.; Cabezon, O (reprint author), Ctr Recerca Sanitat Anim CReSA, IRTA, Campus Univ Autonoma Barcelona, Barcelona, Spain. EM ocabezon@yahoo.com RI Gonzalez-Solis, Jacob/C-3942-2008; Lopez-Soria, Sergio/F-9858-2012; Cabezon, Oscar/L-3812-2014; OI Lopez-Soria, Sergio/0000-0001-7134-2014; Cabezon, Oscar/0000-0001-7543-8371; Fernandez-Aguilar, Xavier/0000-0002-4939-6048 FU Spanish MINECO grant [AGL2012-39830-C02, CGL2009-11278/BOS]; INIA grant [FAU2008-00012-C02-01]; Fondos FEDER funds; FEDER funds FX This study was partially supported by Spanish MINECO grant AGL2012-39830-C02, Spanish MINECO grant CGL2009-11278/BOS, INIA grant FAU2008-00012-C02-01 and Fondos FEDER funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; Thanks for the legal permissions to develop this work to the Dept. de Medi Ambient i Habitatge de la Generalitat de Catalunya; Conselleria de Medi Ambient i Mobilitat del Govern de les Illes Balears; Conselleria de Medi Ambient de la Generalitat Valenciana; Consejeria de Medio Ambiente de la Junta de Andalucia; Conselleria do Medio Rural de la Xunta de Galicia; Parc Natural del Delta de l'Ebre, Parc Natural del Montgri, les Illes Medes i el Baix Ter; Parc Natural illes Columbrets; Parc Natural de sa Dragonera; Parque Nacional Maritimo-Terrestre de las Islas Atlanticas; and to Mariano Paracuellos, Juan Carlos Nevado, Jose Antonio Fernandez Bouzas, Joan Mayol, Jose Vicente Escobar and the guarderia de Columbrets, for their help in logistics and sampling. The authors would also like to thank our many colleagues at University of Barcelona and the staff of CReSA for their help in the field. This study was partially supported by the Spanish MINECO grant AGL2012-39830-C02, CGL2009-11278/BOS and INIA grant FAU2008-00012-C02-01 and FEDER funds. NR 40 TC 1 Z9 1 U1 2 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 14 PY 2016 VL 11 IS 3 AR e0150249 DI 10.1371/journal.pone.0150249 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH1UO UT WOS:000372570600020 PM 26974667 ER PT J AU Yang, SS Fresnedo-Ramirez, J Sun, Q Manns, DC Sacks, GL Mansfield, AK Luby, JJ Londo, JP Reisch, BI Cadle-Davidson, LE Fennell, AY AF Yang, Shanshan Fresnedo-Ramirez, Jonathan Sun, Qi Manns, David C. Sacks, Gavin L. Mansfield, Anna Katharine Luby, James J. Londo, Jason P. Reisch, Bruce I. Cadle-Davidson, Lance E. Fennell, Anne Y. TI Next Generation Mapping of Enological Traits in an F-2 Interspecific Grapevine Hybrid Family SO PLOS ONE LA English DT Article ID POWDERY MILDEW RESISTANCE; DENSITY GENETIC-MAP; VITIS-VINIFERA L.; ENVIRONMENTAL COVARIABLES; AGRONOMIC TRAITS; MALIC-ACID; LOCI; WINE; MARKERS; CONSTRUCTION AB In winegrapes (Vitis spp.), fruit quality traits such as berry color, total soluble solids content (SS), malic acid content (MA), and yeast assimilable nitrogen (YAN) affect fermentation or wine quality, and are important traits in selecting new hybrid winegrape cultivars. Given the high genetic diversity and heterozygosity of Vitis species and their tendency to exhibit inbreeding depression, linkage map construction and quantitative trait locus (QTL) mapping has relied on F-1 families with the use of simple sequence repeat (SSR) and other markers. This study presents the construction of a genetic map by single nucleotide polymorphisms identified through genotyping-by-sequencing (GBS) technology in an F-2 mapping family of 424 progeny derived from a cross between the wild species V. riparia Michx. and the interspecific hybrid winegrape cultivar, 'Seyval'. The resulting map has 1449 markers spanning 2424 cM in genetic length across 19 linkage groups, covering 95% of the genome with an average distance between markers of 1.67 cM. Compared to an SSR map previously developed for this F-2 family, these results represent an improved map covering a greater portion of the genome with higher marker density. The accuracy of the map was validated using the well-studied trait berry color. QTL affecting YAN, MA and SS related traits were detected. A joint MA and SS QTL spans a region with candidate genes involved in the malate metabolism pathway. We present an analytical pipeline for calling intercross GBS markers and a high-density linkage map for a large F-2 family of the highly heterozygous Vitis genus. This study serves as a model for further genetic investigations of the molecular basis of additional unique characters of North American hybrid wine cultivars and to enhance the breeding process by marker-assisted selection. The GBS protocols for identifying intercross markers developed in this study can be adapted for other heterozygous species. C1 [Yang, Shanshan; Reisch, Bruce I.] Cornell Univ, Sch Integrat Plant Sci, Hort Sect, Geneva, NY USA. [Fresnedo-Ramirez, Jonathan; Sun, Qi] Cornell Univ, Inst Biotechnol, Bioinformat Facil, Rhodes Hall, Ithaca, NY USA. [Manns, David C.; Mansfield, Anna Katharine] Cornell Univ, Dept Food Sci, NYSAES, Geneva, NY USA. [Sacks, Gavin L.] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Luby, James J.] Univ Minnesota, Dept Hort Sci, St Paul, MN USA. [Londo, Jason P.; Cadle-Davidson, Lance E.] ARS, USDA, Grape Genet Res Unit, Geneva, NY USA. [Fennell, Anne Y.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Fennell, Anne Y.] BioSNTR, Brookings, SD USA. RP Fennell, AY (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA.; Fennell, AY (reprint author), BioSNTR, Brookings, SD USA. EM Anne.Fennell@sdstate.edu OI Sacks, Gavin/0000-0002-1403-0505; Fresnedo Ramirez, Jonathan/0000-0003-1985-2513 FU United States Department of Agriculture, National Institute of Food and Agriculture Specialty Crops Research Initiative Award [2011-51181-30635] FX This work was supported by the United States Department of Agriculture, National Institute of Food and Agriculture Specialty Crops Research Initiative Award No. 2011-51181-30635, BIR, LCD, AYF, GLS, JJL, AKM. This fund supported JFR and DCM. This work was also supported by the National Science Foundation Award No. IIA1355423, South Dakota Research Innovation Center and a gift from J. Lohr Vineyards & Wines to AYF. SY was supported by a grant from the National Grape and Wine Initiative to BIR. Any opinions, findings, and conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the NSF, USDA or other funding entities. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 75 TC 1 Z9 1 U1 15 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 14 PY 2016 VL 11 IS 3 AR e0149560 DI 10.1371/journal.pone.0149560 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DH1UO UT WOS:000372570600012 PM 26974672 ER PT J AU Hsu, JW Thame, MM Gibson, R Baker, TM Tang, GJ Chacko, SK Jackson, AA Jahoor, F AF Hsu, Jean W. Thame, Minerva M. Gibson, Raquel Baker, Tameka M. Tang, Grace J. Chacko, Shaji K. Jackson, Alan A. Jahoor, Farook TI Unlike pregnant adult women, pregnant adolescent girls cannot maintain glycine flux during late pregnancy because of decreased synthesis from serine SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Pregnant adolescent girls; Pregnant adult women; Glycine; Serine ID AMINO-ACIDS; IN-VIVO; OVINE PLACENTA; HEALTHY-MEN; FETAL LAMB; TURNOVER; METABOLISM; TRANSPORT; PLASMA; FETUS AB During pregnancy, glycine and serine become more important because they are the primary suppliers of methyl groups for the synthesis of fetal DNA, and more glycine is required for fetal collagen synthesis as pregnancy progresses. In an earlier study, we reported that glycine flux decreased by 39% from the first to the third trimester in pregnant adolescent girls. As serine is a primary precursor for glycine synthesis, the objective of this study was to measure and compare glycine and serine fluxes and inter-conversions in pregnant adolescent girls and adult women in the first and third trimesters. Measurements were made after an overnight fast by continuous intravenous infusions of H-2(2)-glycine and N-15-serine in eleven adolescent girls (17.4 (SE 0.1) years of age) and in ten adult women (25.8 (SE 0.5) years of age) for 4 h. Adolescent girls had significantly slower glycine flux and they made less glycine from serine in the third (P < 0.05) than in the first trimester. Baby birth length was significantly shorter of adolescent girls (P=0.04) and was significantly associated with third trimester glycine flux. These findings suggest that the pregnant adolescent cannot maintain glycine flux in late pregnancy compared with early pregnancy because of decreased synthesis from serine. It is possible that the inability to maintain glycine synthesis makes her fetus vulnerable to impaired cartilage synthesis, and thus linear growth. C1 [Hsu, Jean W.; Tang, Grace J.; Chacko, Shaji K.; Jahoor, Farook] Baylor Univ, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Thame, Minerva M.; Baker, Tameka M.] Univ W Indies, Dept Child & Adolescent Hlth, Kingston 7, Jamaica. [Gibson, Raquel] Univ W Indies, Dept Obstet & Gynaecol, Kingston 7, Jamaica. [Jackson, Alan A.] Southampton Gen Hosp, Southampton Biomed Res Ctr, NIHR, MP 113,Tremona Rd, Southampton SO16 6YD, Hants, England. RP Jahoor, F (reprint author), Baylor Univ, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM fjahoor@bcm.edu FU US Department of Agriculture, Agricultural Research Service [58-6250-6001]; International Atomic Energy Agency FX This research was supported with federal funds from the US Department of Agriculture, Agricultural Research Service under Cooperative Agreement Number 58-6250-6001 and funds from the International Atomic Energy Agency. NR 17 TC 0 Z9 0 U1 1 U2 1 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD MAR 14 PY 2016 VL 115 IS 5 BP 759 EP 763 DI 10.1017/S0007114515005279 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DG5XO UT WOS:000372154500002 PM 26785702 ER PT J AU Paudel, B Das, A Tran, M Boe, A Palmer, NA Sarath, G Gonzalez-Hernandez, JL Rushton, PJ Rohila, JS AF Paudel, Bimal Das, Aayudh Tran, Michaellong Boe, Arvid Palmer, Nathan A. Sarath, Gautam Gonzalez-Hernandez, Jose L. Rushton, Paul J. Rohila, Jai S. TI Proteomic Responses of Switchgrass and Prairie Cordgrass to Senescence SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE biofuel grasses; cordgrass; switchgrass; proteomics; senescence ID LEAF SENESCENCE; PHOTOSYSTEM-II; OXIDATIVE STRESS; INTERACTION NETWORKS; BIOMASS PRODUCTION; MOLECULAR-BIOLOGY; INHIBITS GROWTH; TOBACCO PLANTS; CELL-DEATH; ARABIDOPSIS AB Senescence in biofuel grasses is a critical issue because early senescence decreases potential biomass production by limiting aerial growth and development. 2-Dimensional, differential in-gel electrophoresis (2D-DIGE) followed by mass spectrometry of selected protein spots was used to evaluate differences between leaf proteomes of early (ES)- and late-senescing (LS) genotypes of Prairie cordgrass (ES/LS PCG) and switchgrass (ES/LS SG), just before and after senescence was initiated. Analysis of the manually filtered and statistically evaluated data indicated that 69 proteins were significantly differentially abundant across all comparisons, and a majority (41%) were associated with photosynthetic processes as determined by gene ontology analysis. Ten proteins were found in common between PCG and SG, and nine and 18 proteins were unique to PCG and SG respectively. Five of the 10 differentially abundant spots common to both species were increased in abundance, and five were decreased in abundance. Leaf proteomes of the LS genotypes of both grasses analyzed before senescence contained significantly higher abundances of a 14-3-3 like protein and a glutathione-S-transferase protein when compared to the ES genotypes, suggesting differential cellular metabolism in the LS vs. the ES genotypes. The higher abundance of 14-3-3 like proteins may be one factor that impacts the senescence process in both LS PCG and LS SG. Aconitase dehydratase was found in greater abundance in all four genotypes after the onset of senescence, consistent with literature reports from genetic and transcriptomic studies. A Rab protein of the Ras family of G proteins and an s-adenosylmethionine synthase were more abundant in ES PCG when compared with the LS PCG. In contrast, several proteins associated with photosynthesis and carbon assimilation were detected in greater abundance in LS PCG when compared to ES PCG, suggesting that a loss of these proteins potentially contributed to the ES phenotype in PCG. Overall, this study provides important data that can be utilized toward delaying senescence in both PCG and SG, and sets a foundational base for future improvement of perennial grass germplasm for greater aerial biomass productivity. C1 [Paudel, Bimal; Das, Aayudh; Tran, Michaellong; Rohila, Jai S.] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Boe, Arvid; Gonzalez-Hernandez, Jose L.; Rohila, Jai S.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Palmer, Nathan A.; Sarath, Gautam] ARS, Grain Forage & Bioenergy Res Unit, USDA, Lincoln, NE USA. [Rushton, Paul J.] 22nd Century Grp Inc, Clarence, NY USA. [Das, Aayudh] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA. RP Rohila, JS (reprint author), S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA.; Rohila, JS (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM jai.rohila@sdstate.edu FU North Central Regional Sun Grant Center at South Dakota State University through a grant US Department of Agriculture [2010-38502-21861]; USDA-ARS CRIS project [3042-21000030-00D]; CRIS project [SD0011541-15] FX This research was supported by funding from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Agriculture under award number 2010-38502-21861. This work was supported in part by the USDA-ARS CRIS project 3042-21000030-00D and CRIS project SD0011541-15. The U.S. Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. NR 79 TC 0 Z9 0 U1 1 U2 12 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 14 PY 2016 VL 7 AR 293 DI 10.3389/fpls.2016.00293 PG 15 WC Plant Sciences SC Plant Sciences GA DG2US UT WOS:000371925000001 PM 27014316 ER PT J AU Puntsag, T Mitchell, MJ Campbell, JL Klein, ES Likens, GE Welker, JM AF Puntsag, Tamir Mitchell, Myron J. Campbell, John L. Klein, Eric S. Likens, Gene E. Welker, Jeffrey M. TI Arctic Vortex changes alter the sources and isotopic values of precipitation in northeastern US SO SCIENTIFIC REPORTS LA English DT Article ID BROOK EXPERIMENTAL FOREST; STABLE ISOTOPES; CLIMATE-CHANGE; OSCILLATION; DELTA-O-18; ICE; OXYGEN; O-18 AB Altered atmospheric circulation, reductions in Arctic sea ice, ocean warming, and changes in evaporation and transpiration are driving changes in the global hydrologic cycle. Precipitation isotopic (delta O-18 and delta H-2) measurements can help provide a mechanistic understanding of hydrologic change at global and regional scales. To study the changing water cycle in the northeastern US, we examined the longest (1968-2010) record of precipitation isotope values, collected at the Hubbard Brook Experimental Forest in New Hampshire, US (43 degrees 56'N, 71 degrees 45'W). We found a significant reduction in delta O-18 and delta H-2 values over the 43-year record, coupled with a significant increase in d-excess values. This gradual reduction in delta O-18 and delta H-2 values unexpectedly occurred during a period of regional warming. We provide evidence that these changes are governed by the interactions among the Atlantic Multidecadal Oscillation, loss of Arctic sea ice, the fluctuating jet stream, and regular incursions of polar air into the northeastern US. C1 [Puntsag, Tamir; Mitchell, Myron J.] SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. [Campbell, John L.] US Forest Serv, No Res Stn, Durham, NH 03824 USA. [Klein, Eric S.; Welker, Jeffrey M.] Univ Alaska Anchorage, Dept Biol Sci, Anchorage, AK 99508 USA. [Likens, Gene E.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Likens, Gene E.] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA. RP Puntsag, T (reprint author), SUNY Coll Environm Sci & Forestry, Syracuse, NY 13210 USA. EM tpuntsag@syr.edu FU Fulbright Science and Technology Scholarship; NSF MRI [0953271]; National Science Foundation; A.W. Mellon Foundation FX We would like to thank all those associated with the Hubbard Brook Ecosystem Study (HBES) who collected and archived the samples used for this research. Funding for these collections was provided by the National Science Foundation including the LTREB and LTER programs and The A.W. Mellon Foundation. This research was sponsored by the Fulbright Science and Technology Scholarship through a PhD fellowship award to T.P. Analysis for this project was conducted at the UAA Stable Isotope Laboratory supported by a NSF MRI award (0953271) to J.W. The help of Amey Bailey, Patrick McHale and Geoffrey Millard with the sample archives is most appreciated. The authors gratefully acknowledge the NOAA Air Resources Laboratory (ARL) for the provision of the HYSPLIT transport model and/or READY website (http://www.ready.noaa.gov) used in this publication. We thank to Batkhuu Dashzeveg for assistance in providing Arc GIS map. NR 39 TC 2 Z9 2 U1 3 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 14 PY 2016 VL 6 AR 22647 DI 10.1038/srep22647 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG2BL UT WOS:000371871100001 PM 26971874 ER PT J AU Norrbom, AL Savaris, M Marinoni, L AF Norrbom, Allen L. Savaris, Marcoandre Marinoni, Luciane TI New species of Rhagoletotrypeta (Diptera: Tephritidae) from the Dominican Republic and southern Brazil and Paraguay SO ZOOTAXA LA English DT Article DE fruit flies; taxonomy; host plant; Celtis AB Rhagoletotrypeta chapecensis Norrbom & Savaris, new species, and R. gelabertae Norrbom & Savaris, new species, are described and illustrated from specimens from southern Brazil (Parana, Santa Catarina) and Paraguay, and from the Dominican Republic, respectively. The larvae of R. chapecensis develop in fruits of Celtis iguanaea (Jacq.) Sarg. (Ulmaceae). A key to the species of the xanthogastra species group is provided. C1 [Norrbom, Allen L.] USDA ARS, Smithsonian Inst, Systemat Entomol Lab, POB 37012,MRC 168, Washington, DC 20013 USA. [Savaris, Marcoandre; Marinoni, Luciane] Univ Fed Parana, Dept Zool, Jardim Amer, BR-81531980 Curitiba, Parana, Brazil. RP Norrbom, AL (reprint author), USDA ARS, Smithsonian Inst, Systemat Entomol Lab, POB 37012,MRC 168, Washington, DC 20013 USA.; Savaris, M; Marinoni, L (reprint author), Univ Fed Parana, Dept Zool, Jardim Amer, BR-81531980 Curitiba, Parana, Brazil. EM allen.norrbom@ars.usda.gov; plaumannimyia@gmail.com; lmarinoni@ufpr.br RI Marinoni, Luciane /C-5720-2013 FU CNPq [200277/2015-4] FX We thank Lucrecia Rodriguez for assistance with the illustrations. CNPq provided financial support for MS and LM (Process number 200277/2015-4). USDA is an equal opportunity provider and employer. NR 8 TC 0 Z9 0 U1 0 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 11 PY 2016 VL 4088 IS 4 BP 547 EP 554 PG 8 WC Zoology SC Zoology GA DH4UF UT WOS:000372780200005 PM 27394357 ER PT J AU Sabet, JA Park, LK Iyer, LK Tai, AK Koh, GY Pfalzer, AC Parnell, LD Mason, JB Liu, ZH Byun, AJ Crott, JW AF Sabet, Julia A. Park, Lara K. Iyer, Lakshmanan K. Tai, Albert K. Koh, Gar Yee Pfalzer, Anna C. Parnell, Laurence D. Mason, Joel B. Liu, Zhenhua Byun, Alexander J. Crott, Jimmy W. TI Paternal B Vitamin Intake Is a Determinant of Growth, Hepatic Lipid Metabolism and Intestinal Tumor Volume in Female Apc(1638N) Mouse Offspring SO PLOS ONE LA English DT Article ID MATERNAL METHYL SUPPLEMENTS; HIGH-FAT DIET; DNA METHYLATION; GENE-EXPRESSION; METASTABLE EPIALLELES; FOLIC-ACID; RNA-SEQ; MICE; FOLATE; OBESITY AB Background The importance of maternal nutrition to offspring health and risk of disease is well established. Emerging evidence suggests paternal diet may affect offspring health as well. Objective In the current study we sought to determine whether modulating pre-conception paternal B vitamin intake alters intestinal tumor formation in offspring. Additionally, we sought to identify potential mechanisms for the observed weight differential among offspring by profiling hepatic gene expression and lipid content. Methods Male Apc(1638N) mice (prone to intestinal tumor formation) were fed diets containing replete (control, CTRL), mildly deficient (DEF), or supplemental (SUPP) quantities of vitamins B-2, B-6, B-12, and folate for 8 weeks before mating with control-fed wild type females. Wild type offspring were euthanized at weaning and hepatic gene expression profiled. Apc(1638N) offspring were fed a replete diet and euthanized at 28 weeks of age to assess tumor burden. Results No differences in intestinal tumor incidence or burden were found between male Apc(1638N) offspring of different paternal diet groups. Although in female Apc(1638N) offspring there were no differences in tumor incidence or multiplicity, a stepwise increase in tumor volume with increasing paternal B vitamin intake was observed. Interestingly, female offspring of SUPP and DEF fathers had a significantly lower body weight than those of CTRL fed fathers. Moreover, hepatic trigylcerides and cholesterol were elevated 3-fold in adult female offspring of SUPP fathers. Weanling offspring of the same fathers displayed altered expression of several key lipid-metabolism genes. Hundreds of differentially methylated regions were identified in the paternal sperm in response to DEF and SUPP diets. Aside from a few genes including Igf2, there was a striking lack of overlap between these genes differentially methylated in sperm and differentially expressed in offspring. Conclusions In this animal model, modulation of paternal B vitamin intake prior to mating alters offspring weight gain, lipid metabolism and tumor growth in a sex-specific fashion. These results highlight the need to better define how paternal nutrition affects the health of offspring. C1 [Sabet, Julia A.; Park, Lara K.; Koh, Gar Yee; Pfalzer, Anna C.; Parnell, Laurence D.; Mason, Joel B.; Liu, Zhenhua; Byun, Alexander J.; Crott, Jimmy W.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Sabet, Julia A.; Park, Lara K.; Pfalzer, Anna C.; Mason, Joel B.; Crott, Jimmy W.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Iyer, Lakshmanan K.] Tufts Univ, Sch Med, Tufts Ctr Neurosci Res, Neurosci Dept, Boston, MA 02111 USA. [Iyer, Lakshmanan K.] Tufts Med Ctr, Mol Cardiol Res Inst, Boston, MA USA. [Tai, Albert K.] Tufts Univ, Sch Med, Tufts Univ Core Facil, Boston, MA 02111 USA. [Liu, Zhenhua] UMass Amherst, Sch Publ Hlth & Hlth Sci, Amherst, MA USA. RP Crott, JW (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA.; Crott, JW (reprint author), Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. EM jimmy.crott@tufts.edu FU NCI (National Cancer Institute) [1 R03CA162505-1]; USDA-ARS [58-1950-0-014, 58-1950-4-003] FX Funding was provided by NCI (National Cancer Institute) 1 R03CA162505-1 and USDA-ARS #58-1950-0-014 and ##58-1950-4-003. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 57 TC 5 Z9 5 U1 2 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 11 PY 2016 VL 11 IS 3 AR e0151579 DI 10.1371/journal.pone.0151579 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG3SC UT WOS:000371989200064 PM 26968002 ER PT J AU Castro, EB Feres, RJF Ochoa, R Bauchan, GR AF Castro, Elizeu B. Feres, Reinaldo J. F. Ochoa, Ronald Bauchan, Gary R. TI A new species of Tenuipalpus sensu stricto (Acari: Tenuipalpidae) from Brazil, with ontogeny and a key to the known species SO ZOOTAXA LA English DT Article DE flat mites; Tenuipalpus spinosaurus; crest; body projections; taxonomic; systematic; biodiversity; Cerrado; LT-SEM ID MITES ACARI; CERRADO; MYRTACEAE; STATE AB The Cerrado is the second largest Brazilian biome, and is considered to be a "hotspot" due the great concentration of endemic species and high rate of deforestation. Surveys of the mite fauna present in this biome have revealed a great number of new species. In this paper, we describe Tenuipalpus spinosaurus sp. nov. (Acari: Tenuipalpidae), a new species of Tenuipalpus sensu stricto, from adult females, deutonymphs, protonymphs, larvae and eggs, collected on Terminalia argentea (Combretaceae), from the Cerrado in Brazil. Females of this new species bear a prominent longitudinal crest on the opisthosoma. The ontogenetic changes in the idiosoma and leg chaetotaxy of all stages are presented. A key to the world species of Tenuipalpus sensu stricto is provided. C1 [Castro, Elizeu B.] Univ Estadual Paulista, Programa Posgrad Biol Anim, Campus Sao Jose Rio Preto, Sao Paulo, Brazil. [Feres, Reinaldo J. F.] UNESP, Dept Zool & Bot, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, Brazil. [Feres, Reinaldo J. F.] CNPq Brazil Researcher, Sao Paulo, Brazil. [Ochoa, Ronald] USDA ARS, Beltsville Agr Res Ctr, SEL, Beltsville, MD 20705 USA. [Bauchan, Gary R.] USDA ARS, BARC, ECMU, Beltsville, MD 20705 USA. RP Castro, EB (reprint author), Univ Estadual Paulista, Programa Posgrad Biol Anim, Campus Sao Jose Rio Preto, Sao Paulo, Brazil. EM elizeu_unesp@yahoo.com.br; reinaldo@ibilce.unesp.br; ron.ochoa@ars.usda.gov; gary.bauchan@ars.usda.gov RI Castro, Elizeu/A-9915-2015 OI Castro, Elizeu/0000-0002-8056-6893 FU Smithsonian Natural History Museum; National Agricultural Library (NAL-USDA); USDA National Program; APHIS; "Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior" (CAPES) [BEX 7768/13-3]; "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq) [303435/2013-5] FX We offer special thanks to Nit Malikul and Debra Creel (SEL-USDA) for their technical support; to Chris Pooley (ECMU-USDA) for his help with the LT-SEM images; to Dr. Antonio C. Lofego (UNESP-Sao Jose do Rio Preto) and MSc. Jose M. Rezende for his help in the collecting of specimens for LT-SEM. In addition, we wish to thank to Dr. Laura Leibensperger, Department of Invertebrate Zoology, Museum of Comparative Zoology - (MCZ), Harvard University; Dr. Gilberto J. de Moraes and Dr. Carlos H.W. Flechtmann, The Acarology Collection, USP-ESALQ, for the lending of valuable specimens. We give a special thank you to Drs. Gregory Evans, Jennifer Beard and Owen Seeman for their review of the manuscript and helpful suggestions. We thank the Smithsonian Natural History Museum, National Agricultural Library (NAL-USDA), USDA National Program and APHIS for their support and assistance with references, permits and funding for this study. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer. This study was supported by the "Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior" (CAPES) (Proc. no. BEX 7768/13-3) and "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq) (Proc. no. 303435/2013-5), with a fellowship and research grant for the first and second authors, respectively. NR 18 TC 0 Z9 0 U1 2 U2 3 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD MAR 10 PY 2016 VL 4088 IS 3 BP 355 EP 378 PG 24 WC Zoology SC Zoology GA DH4TV UT WOS:000372779200003 PM 27394345 ER PT J AU Artlip, TS Wisniewski, ME Arora, R Norelli, JL AF Artlip, Timothy S. Wisniewski, Michael E. Arora, Rajeev Norelli, John L. TI An apple rootstock overexpressing a peach CBF gene alters growth and flowering in the scion but does not impact cold hardiness or dormancy SO HORTICULTURE RESEARCH LA English DT Article ID ENHANCES FREEZING TOLERANCE; ABIOTIC STRESS RESPONSES; TRANSCRIPTION FACTORS; LOW-TEMPERATURE; DELLA PROTEINS; REGULATORY NETWORKS; SIGNALING PATHWAY; ERWINIA-AMYLOVORA; WOODY-PLANTS; ARABIDOPSIS AB The C-repeat binding factor (CBF) transcription factor is involved in responses to low temperature and water deficit in many plant species. Overexpression of CBF genes leads to enhanced freezing tolerance and growth inhibition in many species. The overexpression of a peach CBF (PpCBF1) gene in a transgenic line of own-rooted apple (Malus x domestica) M. 26 rootstock (T166) trees was previously reported to have additional effects on the onset of dormancy and time of spring budbreak. In the current study, the commercial apple cultivar 'Royal Gala' (RG) was grafted onto either non-transgenic M. 26 rootstocks (RG/M. 26) or transgenic M. 26 (T166) rootstocks (RG/T166) and field grown for 3 years. No PpCBF1 transcript was detected in the phloem or cambium of RG scions grafted on T166 rootstocks indicating that no graft transmission of transgene mRNA had occurred. In contrast to own-rooted T166 trees, no impact of PpCBF1 overexpression in T166 rootstocks was observed on the onset of dormancy, budbreak or non-acclimated leaf-cold hardiness in RG/T166 trees. Growth, however, as measured by stem caliper, current-year shoot extension and overall height, was reduced in RG/T166 trees compared with RG/M. 26 trees. Although flowering was evident in both RG/T166 and RG/M. 26 trees in the second season, the number of trees in flower, the number of shoots bearing flowers, and the number of flower clusters per shoot was significantly higher in RG/M. 26 trees than RG/T166 trees in both the second and third year after planting. Elevated levels of RGL (DELLA) gene expression were observed in RG/T166 trees and T166 trees, which may play a role in the reduced growth observed in these tree types. A model is presented indicating how CBF overexpression in a rootstock might influence juvenility and flower abundance in a grafted scion. C1 [Artlip, Timothy S.; Wisniewski, Michael E.; Norelli, John L.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. [Arora, Rajeev] Iowa State Univ, Dept Hort, Ames, IA USA. RP Wisniewski, ME (reprint author), ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. EM michael.wisniewski@ars.usda.gov NR 55 TC 0 Z9 0 U1 9 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-7276 J9 HORTIC RES-ENGLAND JI Hortic. Res.-England PD MAR 9 PY 2016 VL 3 AR 16006 DI 10.1038/hortres.2016.6 PG 9 WC Plant Sciences; Horticulture SC Plant Sciences; Agriculture GA DJ9GV UT WOS:000374522900001 PM 26981253 ER PT J AU Devaiah, SP Owens, DK Sibhatu, MB Sarkar, TR Strong, CL Mallampalli, VKPS Asiago, J Cooke, J Kiser, S Lin, ZF Wamucho, A Hayford, D Williams, BE Loftis, P Berhow, M Pike, LM McIntosh, CA AF Devaiah, Shivakumar P. Owens, Daniel K. Sibhatu, Mebrahtu B. Sarkar, Tapasree Roy Strong, Christy L. Mallampalli, Venkata K. P. S. Asiago, Josephat Cooke, Jennifer Kiser, Starla Lin, Zhangfan Wamucho, Anye Hayford, Deborah Williams, Bruce E. Loftis, Peri Berhow, Mark Pike, Lee M. McIntosh, Cecilia A. TI Identification, Recombinant Expression, and Biochemical Analysis of Putative Secondary Product Glucosyltransferases from Citrus paradisi SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Citrus paradisi; glucosyltransferase; flavonoid; heterologous expression; flavor ID GLUCOSE-ANTHOCYANIDIN 3-O-GLUCOSYLTRANSFERASE; GRAPE VITIS-LABRUSCA; UDP-GLUCOSE; MOLECULAR-CLONING; FLAVONOID 3-O-GLUCOSYLTRANSFERASE; BETANIDIN 5-O-GLUCOSYLTRANSFERASE; SUBSTRATE-SPECIFICITY; CRYSTAL-STRUCTURES; IRIS-HOLLANDICA; PETUNIA-HYBRIDA AB Flavonoid and limonoid glycosides influence taste properties as well as marketability of Citrus fruit and products, particularly grapefruit. In this work, nine grapefruit putative natural product glucosyltransferases (PGTs) were resolved by either using degenerate primers against the semiconserved PSPG box motif, SMART-RACE RT-PCR, and primer walking to full-length coding regions; screening a directionally cloned young grapefruit leaf EST library; designing primers against sequences from other Citrus species; or identifying PGTs from Citrus contigs in the harvEST database. The PGT proteins associated with the identified full-length coding regions were recombinantly expressed in Escherichia coli and/or Pichia pastoris and then tested for activity with a suite,of substrates including flavonoid, simple phenolic, coumarin, and/or limonoid compounds. A number of these compounds were eliminated from the predicted and/or potential substrate pool for the identified PGTs. Enzyme activity was detected in some instances with quercetin and catechol glucosyltransferase activities having been identified. C1 [Devaiah, Shivakumar P.; Sibhatu, Mebrahtu B.; Sarkar, Tapasree Roy; Strong, Christy L.; Mallampalli, Venkata K. P. S.; Asiago, Josephat; Cooke, Jennifer; Kiser, Starla; Lin, Zhangfan; Wamucho, Anye; Hayford, Deborah; Williams, Bruce E.; Loftis, Peri; Pike, Lee M.; McIntosh, Cecilia A.] E Tennessee State Univ, Dept Biol Sci, POB 70703, Johnson City, TN 37614 USA. [McIntosh, Cecilia A.] E Tennessee State Univ, Sch Grad Studies, POB 70720, Johnson City, TN 37614 USA. [Owens, Daniel K.] ARS, Nat Prod Utilizat Res Unit, USDA, POB 1848, University, MS 38677 USA. [Berhow, Mark] ARS, Funct Foods Res Unit, USDA, Peoria, IL 61604 USA. [Sibhatu, Mebrahtu B.] ImClone Syst, 450 E 29th St, New York, NY 10016 USA. [Sarkar, Tapasree Roy] Univ Texas MD Anderson Canc Ctr, 1515 Holcombe Blvd, Houston, TX 77030 USA. [Strong, Christy L.] Univ Nevada, Sch Life Sci, Box 4004,4505 S Maryland Pkwy, Las Vegas, NV 89154 USA. [Mallampalli, Venkata K. P. S.] Univ Texas Hlth Sci Ctr Houston, 7000 Fannin,Suite 1200, Houston, TX 77030 USA. RP McIntosh, CA (reprint author), E Tennessee State Univ, Dept Biol Sci, POB 70703, Johnson City, TN 37614 USA.; McIntosh, CA (reprint author), E Tennessee State Univ, Sch Grad Studies, POB 70720, Johnson City, TN 37614 USA. EM mcintosc@etsu.edu FU USDA [2003-35318-13749]; NSF MCB Grants [0614260, 1120268]; NSF MCB REU supplements [0734216, 0829613, 1228176, 1418501]; Sigma Xi GIAR FX This work was supported by USDA Grant 2003-35318-13749 awarded to C.A.M. and L.M.P.; NSF MCB Grants 0614260 and 1120268 and NSF MCB REU supplements 0734216, 0829613, 1228176, and 1418501 awarded to C.A.M.; and a Sigma Xi GIAR awarded to T.R.S. NR 74 TC 2 Z9 2 U1 6 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 9 PY 2016 VL 64 IS 9 BP 1957 EP 1969 DI 10.1021/acs.jafc.5b05430 PG 13 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DG3BW UT WOS:000371945500011 PM 26888166 ER PT J AU Yi, J Fan, YT Yokoyama, W Zhang, YZ Zhao, LQ AF Yi, Jiang Fan, Yuting Yokoyama, Wallace Zhang, Yuzhu Zhao, Liqing TI Thermal Degradation and Isomerization of beta-Carotene in Oil-in-Water Nanoemulsions Supplemented with Natural Antioxidants SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE beta-carotene; nanoemulsion; degradation; isomerization; antioxidant ID CHEMICAL-STABILITY; CELLULAR UPTAKE; ALL-TRANS; SWEET-POTATOES; SINGLET OXYGEN; EMULSIONS; OXIDATION; LYCOPENE; ISOMERS; VITRO AB The goal of this study was to see the impact on the retention and isomerization of encapsulated beta-carotene (BC) in nanoemulsions fortified with natural antioxidants (alpha-tocopherol (AT) and L-ascorbic acid (AA)). The physical stability of nanoemulsion, oxidative stability, and isomerization of all-trans-beta-carotene (BC) in oil-in-water (O/W) nanoemulsions were determined in the presence or absence of natural antioxidants at 25 and 50 degrees C at certain intervals of time by high-performance liquid chromatography (HPLC). Sodium caseinate was used as the emulsifier, and corn oil (CO) was more protective than medium-chain triglycerides (MCT) and used for isomerization studies. Mean diameters of control (without antioxidants) and AA- and AT-fortified particles were similar. Mean particle diameter of nanoemulsions increased from 10 to 25 nm at 25 degrees C and from 40 to 50 nm at 50 degrees C during 30 days of storage. The isomerization from all-trans-BC to cis-BC isomers was inhibited by antioxidants. The isomerization rates were in the following order: 13-cis-BC > 15-cis-BC > 9-cis-BC. AT had better antioxidant activities than AA in inhibiting BC degradation in O/W nanoemulsions. The results indicated that BC encapsulated in nanoemulsions supplemented with antioxidants could significantly improve BC's chemical stability. C1 [Yi, Jiang; Zhao, Liqing] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. [Fan, Yuting] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China. [Yokoyama, Wallace; Zhang, Yuzhu] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Yi, J; Zhao, LQ (reprint author), Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. EM yijiangjnu@gmail.com; snowyzlj@163.com FU Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program [JCYJ20140418091413576, CXZZ20150430093131635] FX This work was supported by the Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program (JCYJ20140418091413576, CXZZ20150430093131635). NR 40 TC 2 Z9 2 U1 15 U2 29 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 9 PY 2016 VL 64 IS 9 BP 1970 EP 1976 DI 10.1021/acs.jafc.5b05478 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DG3BW UT WOS:000371945500012 PM 26881704 ER PT J AU Lyons, EJ Baranowski, T Basen-Engquist, KM Lewis, ZH Swartil, MC Jennings, K Volpi, E AF Lyons, Elizabeth J. Baranowski, Torn Basen-Engquist, Karen M. Lewis, Zakkoyya H. Swartil, Maria C. Jennings, Kristofer Volpi, Elena TI Testing the effects of narrative and play on physical activity among breast cancer survivors using mobile apps: study protocol for a randomized controlled trial SO BMC CANCER LA English DT Article DE Physical activity; Breast cancer; Cancer survivorship; Video games; Narrative; mhealth; Intervention; Mobile app ID QUALITY-OF-LIFE; BEHAVIOR-CHANGE INTERVENTIONS; SELF-DETERMINATION THEORY; AFRICAN-AMERICAN WOMEN; CORONARY-HEART-DISEASE; FUNCTIONAL ASSESSMENT; OBESITY PREVENTION; HEALTH DISPARITIES; EXERCISE IMAGERY; MENTAL-IMAGERY AB Background: Physical activity reduces risk for numerous negative health outcomes, but postmenopausal breast cancer survivors do not reach recommended levels. Many interventions encourage self-monitoring of steps, which can increase physical activity in the short term. However, these interventions appear insufficient to increase motivation for sustained change. There is a need for innovative strategies to increase physical activity motivation in this population. Narratives are uniquely persuasive, and video games show promise for increasing motivation. This study will determine the effectiveness of an intervention that combines narrative and gaming to encourage sustained physical activity. Methods/Design: SMARTGOAL (Self -Monitoring Activity: a Randomized Trial of Game-Oriented Applications) is a randomized controlled intervention trial. The intervention period is six months, followed by a six month maintenance period. Participants (overweight, sedentary postmenopausal breast cancer survivors aged 45-75) will be randomized to a self-monitoring group or an enhanced narrative game group. The self -monitoring group will be encouraged to use a mobile application for self -monitoring and feedback and will receive 15 counseling phone calls emphasizing self-regulation. The narrative game group will be encouraged to use a mobile application that includes self -monitoring and feedback as well as a narrative-based active video game. The 15 calls for this group will emphasize concepts related to the game storyline. Counseling calls in both groups will occur weekly in months 1 3 and monthly in months 4 6. No counseling calls will occur after month 6, but both groups will be encouraged to continue using their apps. The primary outcome of the study is minutes of moderate to vigorous physical activity at six months. Other objectively measured outcomes include fitness and physical function. Self-reported outcomes include quality of life, depression, and motivation. Discussion: This protocol will result in implementation and evaluation of two technology-based physical activity interventions among breast cancer survivors. Both interventions hold promise for broad dissemination. Understanding the potential benefit of adding narrative and game elements to interventions will provide critical information to interventionists, researchers, clinicians, and policymakers. This study is uniquely suited to investigate not just whether but how and why game elements may improve breast cancer survivors' health. C1 [Lyons, Elizabeth J.] Univ Texas Med Branch, Dept Nutr & Metab, 301 Univ Blvd, Galveston, TX 77555 USA. [Baranowski, Torn] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. [Basen-Engquist, Karen M.] Univ Texas MD Anderson Canc Ctr, Dept Behav Sci, 1155 Pressler St, Houston, TX 77030 USA. [Basen-Engquist, Karen M.] Univ Texas MD Anderson Canc Ctr, Ctr Energy Balance Canc Prevent & Survivorship, 1155 Pressler St, Houston, TX 77030 USA. [Lewis, Zakkoyya H.; Swartil, Maria C.] Univ Texas Med Branch, Div Rehabil Sci, 301 Univ Blvd, Galveston, TX 77555 USA. [Jennings, Kristofer] Univ Texas Med Branch, Dept Prevent Med & Community Hlth, 301 Univ Blvd, Galveston, TX 77555 USA. [Volpi, Elena] Univ Texas Med Branch, Dept Geriatr, 301 Univ Blvd, Galveston, TX 77555 USA. [Volpi, Elena] Univ Texas Med Branch, Claude D Pepper Older Americans Independence Ctr, 301 Univ Blvd, Galveston, TX 77555 USA. RP Lyons, EJ (reprint author), Univ Texas Med Branch, Dept Nutr & Metab, 301 Univ Blvd, Galveston, TX 77555 USA. EM eyons@utmb.edu RI Emchi, Karma/Q-1952-2016; OI Baranowski, Tom/0000-0002-0653-2222; Lyons, Elizabeth/0000-0003-1695-2236 FU American Cancer Society [MRSG-14-165-01-CPPB]; American Heart Association [13BGIA17110021]; National Cancer Institute [K07CA175141, CA 172864, CA158917]; National Center for Minority Health and Disease [MD005814]; National Institute of Diabetes Digestive and Kidney Disease [DK091254-01]; National Institute of Child Health and Human Development [HD075521, HD073608]; USDA/ARS [3092-5-001-058]; MD Anderson Cancer Center support grant [P30 CA016672]; Center for Energy Balance in Cancer Prevention and Survivorship, Duncan Family Institute for Cancer Prevention and Risk Assessment; Cancer Prevention Research Institute of Texas [PP130079]; Comparative Effectiveness Research on Cancer in Texas from the Cancer Prevention Research Institute of Texas [RP140020]; National Institute on Disability and Rehabilitation Research, Department of Education [H133P110012]; Claude D. Pepper Older Americans Independence Center at UTMB [P30AG024832]; Institute for Translational Sciences - Clinical and Translational Science Award from the National Center for Advancing Translational Sciences, National Institutes of Health [UL1TR000071]; Sealy Center on Aging at the University of Texas Medical Branch; US Agency for Healthcare Research and Quality (AHRQ) [R24HS22134] FX Elizabeth Lyons, PhD, was supported by a Mentored Research Scholar Grant in Applied and Clinical Research, MRSG-14-165-01-CPPB, from the American Cancer Society. Drs. Lyons and Swartz were also supported by a Beginning Grant-in-Aid, 13BGIA17110021, from the American Heart Association. Creation of the counseling content was supported by National Cancer Institute grant K07CA175141.; Tom Baranowski was primarily funded by several grants, including "Using Technology to Prevent Obesity among African American Girls" (MD005814) from the National Center for Minority Health and Disease; "Video Games for Obesity & Diabetes Prevention: Efficacy Trial" (DK091254-01) from the National Institute of Diabetes Digestive and Kidney Disease; "Kiddio Food Fight: Training parents in Effective Vegetable Parenting" (HD075521) from the National Institute of Child Health and Human Development; "Minimizing Memory Errors in Child Diet Assessment" (CA 172864) from the National Cancer Institute; "Narrative Impact of Active Video Games On Physical Activity" (CA158917) from the National Cancer Institute; and "Motivational Theatre to Increase Vegetable Intake in Children" (HD073608) from the National Institute of Child Health and Human Development. This work is also a publication of the U.S. Department of Agriculture, funded in part with federal funds from the USDA/ARS under Cooperative Agreement number 3092-5-001-058.; Dr. Basen-Engquist was supported primarily by the MD Anderson Cancer Center support grant (P30 CA016672); Center for Energy Balance in Cancer Prevention and Survivorship, Duncan Family Institute for Cancer Prevention and Risk Assessment; and a prevention grant from the Cancer Prevention Research Institute of Texas, Active Living Alter Breast Cancer: Combining a Physical Activity Program with Survivor Navigation (PP130079).; Dr. Swartz was also supported by Comparative Effectiveness Research on Cancer in Texas from the Cancer Prevention Research Institute of Texas (RP140020) and the National Institute on Disability and Rehabilitation Research, Department of Education (H133P110012).; Dr. Jennings and Dr. Volpi were primarily supported by the Claude D. Pepper Older Americans Independence Center at UTMB (P30AG024832).; This study was conducted with the support of the Institute for Translational Sciences, supported in part by a Clinical and Translational Science Award (UL1TR000071) from the National Center for Advancing Translational Sciences, National Institutes of Health, and from the Sealy Center on Aging at the University of Texas Medical Branch. This publication was also supported in part by the US Agency for Healthcare Research and Quality (AHRQ) through Grant R24HS22134. NR 95 TC 3 Z9 3 U1 13 U2 29 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2407 J9 BMC CANCER JI BMC Cancer PD MAR 9 PY 2016 VL 16 AR 202 DI 10.1186/s12885-016-2244-y PG 18 WC Oncology SC Oncology GA DF9HQ UT WOS:000371672100003 PM 26960972 ER PT J AU Brown, DM Lampe, AT Workman, AM AF Brown, Deborah M. Lampe, Anna T. Workman, Aspen M. TI The Differentiation and Protective Function of Cytolytic CD4 T Cells in influenza infection SO FRONTIERS IN IMMUNOLOGY LA English DT Review DE CD4 effectors; CD4 cytolytic cells; influenza A virus; perforin; granzyme B ID VIRUS-INFECTION; TRANSCRIPTIONAL CONTROL; MEDIATED PROTECTION; LETHAL INFLUENZA; VIRAL-INFECTION; OLDER-ADULTS; MEMORY; EFFECTOR; PERFORIN; IMMUNITY AB CD4 T cells that recognize peptide antigen in the context of class II MHC can differentiate into various subsets that are characterized by their helper functions. However, increasing evidence indicates that CD4 cells with direct cytolytic activity (CD4 CTL) play a role in chronic as well as acute infections, such as influenza A virus (IAV) infection. In the last couple of decades, techniques to measure the frequency and activity of these cytolytic cells has demonstrated their abundance in infections, such as human immunodeficiency virus, mouse pox, murine gamma herpes virus, cytomegalovirus, Epstein-Barr virus, and influenza among others. We now appreciate a greater role for CD4 CTL as direct effectors in viral infections and antitumor immunity through their ability to acquire perforin-mediated cytolytic activity and contribution to lysis of virally infected targets or tumors. As early as the 1980s, CD4 T cell clones with cytolytic potential were identified after influenza virus infection, yet much of this early work was dependent on in vitro culture and little was known about the physiological relevance of CD4 CTL. Here, we discuss the direct role CD4 CTL play in protection against lethal IAV infection and the factors that drive the generation of perforin-mediated lytic activity in CD4 cells in vivo during IAV infection. While focusing on CD4 CTL generated during IAV infection, we pull comparisons from the literature in other antiviral and antitumor systems. Further, we highlight what is currently known about CD4 CTL secondary and memory responses, as well as vaccination strategies to induce these potent killer cells that provide an extra layer of cell-mediated immune protection against heterosubtypic IAV infection. C1 [Brown, Deborah M.; Lampe, Anna T.] Univ Nebraska, Sch Biol Sci, Lincoln, NE USA. [Brown, Deborah M.; Lampe, Anna T.; Workman, Aspen M.] Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE USA. [Workman, Aspen M.] USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Brown, DM (reprint author), Univ Nebraska, Sch Biol Sci, Lincoln, NE USA.; Brown, DM (reprint author), Univ Nebraska, Nebraska Ctr Virol, Lincoln, NE USA. EM dbrown7@unl.edu FU Public Health Service grants from National Institute of General Medical Sciences [P30GM103509-03]; National Institute of Immunology, Allergy and Infectious Disease [R21-AI090438, R56-100929]; University of Nebraska Research Council FX This work was supported by Public Health Service grants from the National Institute of General Medical Sciences (P30GM103509-03), National Institute of Immunology, Allergy and Infectious Disease (R21-AI090438 and R56-100929), and the University of Nebraska Research Council. NR 52 TC 5 Z9 5 U1 0 U2 1 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-3224 J9 FRONT IMMUNOL JI Front. Immunol. PD MAR 9 PY 2016 VL 7 AR 93 DI 10.3389/fimmu.2016.00093 PG 8 WC Immunology SC Immunology GA DF8VZ UT WOS:000371639800001 PM 27014272 ER PT J AU Wolff, PL Schroeder, C McAdoo, C Cox, M Nelson, DD Evermann, JF Ridpath, JF AF Wolff, Peregrine L. Schroeder, Cody McAdoo, Caleb Cox, Mike Nelson, Danielle D. Evermann, James F. Ridpath, Julia F. TI Evidence of Bovine viral diarrhea virus Infection in Three Species of Sympatric Wild Ungulates in Nevada: Life History Strategies May Maintain Endemic Infections in Wild Populations SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE bovine viral diarrhea virus; bighorn sheep; mountain goat; mule deer; Nevada; Odocoileus hemionus; Oreamnos americanum; Ovis canadensis ID WHITE-TAILED DEER; ODOCOILEUS-VIRGINIANUS; SEROLOGIC SURVEY; BIGHORN SHEEP; PATHOGENS; ANTIBODIES; PNEUMONIA; CERVIDS; TYPE-1; FAWNS AB Evidence for bovine viral diarrhea virus (BVDV) infection was detected in 2009-2010 while investigating a pneumonia die-off in Rocky Mountain bighorn sheep (Ovis canadensis, canadensis), and sympatric mountain goats (Oreamnos americanum) in adjacent mountain ranges in Elko County, Nevada. Seroprevalence to BVDV-1 was 81% (N = 32) in the bighorns and 100% (N = 3) in the mountain goats. Serosurveillance from 2011 to 2015 of surviving bighorns and mountain goats as well as sympatric mule deer (Odocoileus hemionus), indicated a prevalence of 72% (N = 45), 45% (N = 51), and 51% (N = 342) respectively. All species had antibody titers to BVDV1 and BVDV2. BVDV1 was isolated in cell culture from three bighorn sheep and a mountain goat kid. BVDV2 was isolated from two mule deer. Six deer (N = 96) sampled in 2013 were positive for BVDV by antigen capture ELISA on a single ear notch. Wild ungulates and cattle concurrently graze public and private lands in these two mountain ranges, thus providing potential for interspecies viral transmission. Like cattle, mule deer, mountain goats, and bighorn sheep can be infected with BVDV and can develop clinical disease including immunosuppression. Winter migration patterns that increase densities and species interaction during the first and second trimester of gestation may contribute to the long term maintenance of the virus in these wild ungulates. More studies are needed to determine the population level impacts of BVDV infection on these three species. C1 [Wolff, Peregrine L.; Schroeder, Cody; Cox, Mike] Nevada Dept Wildlife, Reno, NV USA. [McAdoo, Caleb] Nevada Dept Wildlife, Elko, NV USA. [Nelson, Danielle D.] Washington State Univ, Coll Vet Med, Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Evermann, James F.] Washington State Univ, Coll Vet Med, Vet Clin Med, Pullman, WA 99164 USA. [Evermann, James F.] Washington State Univ, Coll Vet Med, Washington Anim Dis Diagnost Lab, Pullman, WA 99164 USA. [Ridpath, Julia F.] USDA ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA USA. RP Wolff, PL (reprint author), Nevada Dept Wildlife, Reno, NV USA. EM pwolff@ndow.org FU Nevada Department of Wildlife by USFWS Federal Aid Wildlife Restoration Grant [W-48-R-41]; Barrick Gold Corporation; Elko Bighorns Unlimited; Nevada Bighorns Unlimited; Nevada Wildlife Heritage Trust [13-06] FX This work was supported through the Nevada Department of Wildlife by USFWS Federal Aid Wildlife Restoration Grant W-48-R-41. Additional funding was received from the Barrick Gold Corporation, Elko Bighorns Unlimited, Nevada Bighorns Unlimited and Nevada Wildlife Heritage Trust Account, Grant (#13-06). NR 38 TC 2 Z9 2 U1 9 U2 20 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAR 9 PY 2016 VL 7 AR 297 DI 10.3389/fmicb.2016.00292 PG 8 WC Microbiology SC Microbiology GA DF7NL UT WOS:000371544300001 ER PT J AU Rossman, AY Johnston, P AF Rossman, Amy Y. Johnston, Peter TI (2411-2414) Proposals to conserve the names Pseudopeziza jaapii (Blumeriella jaapii) against Cylindrosporium padi; Pyrenopeziza medicaginis (Leptotrochila medicaginis) against Sporonema phacidioides; Peziza cinnamomea (Pezicula cinnamomea) against Naemaspora grisea; and Pyrenopeziza brassicae against Cylindrosporium concentricum (Ascomycota: Leotiomycetes) SO TAXON LA English DT Editorial Material C1 [Rossman, Amy Y.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Johnston, Peter] Landcare Res, Private Bag 92170, Auckland 1142, New Zealand. RP Rossman, AY (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. EM amydianer@yahoo.com NR 0 TC 0 Z9 0 U1 2 U2 2 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD MAR 8 PY 2016 VL 65 IS 1 BP 184 EP 185 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DH5AG UT WOS:000372796500017 ER PT J AU Schroyen, M Eisley, C Koltes, JE Fritz-Waters, E Choi, I Plastow, GS Guan, LL Stothard, P Bao, H Kommadath, A Reecy, JM Lunney, JK Rowland, RRR Dekkers, JCM Tuggle, CK AF Schroyen, Martine Eisley, Christopher Koltes, James E. Fritz-Waters, Eric Choi, Igseo Plastow, Graham S. Guan, Leluo Stothard, Paul Bao, Hua Kommadath, Arun Reecy, James M. Lunney, Joan K. Rowland, Robert R. R. Dekkers, Jack C. M. Tuggle, Christopher K. TI Bioinformatic analyses in early host response to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) reveals pathway differences between pigs with alternate genotypes for a major host response QTL SO BMC GENOMICS LA English DT Article DE Pig; PRRS virus; RNA-seq; WUR; GBP5; Ion transport; Homeostasis; Regulatory factors ID UBIQUITIN-PROTEASOME SYSTEM; CALCIUM-SENSING RECEPTOR; CELL-CYCLE PROGRESSION; GENE-EXPRESSION DATA; NETWORK ANALYSIS; RNA-SEQ; S-PHASE; PROTEIN; INFECTION; BINDING AB Background: A region on Sus scrofa chromosome 4 (SSC4) surrounding single nucleotide polymorphism (SNP) marker WUR10000125 (WUR) has been reported to be strongly associated with both weight gain and serum viremia in pigs after infection with PRRS virus (PRRSV). A proposed causal mutation in the guanylate binding protein 5 gene (GBP5) is predicted to truncate the encoded protein. To investigate transcriptional differences between WUR genotypes in early host response to PRRSV infection, an RNA-seq experiment was performed on globin depleted whole blood RNA collected on 0, 4, 7, 10 and 14 days post-infection (dpi) from eight littermate pairs with one AB (favorable) and one AA (unfavorable) WUR genotype animal per litter. Results: Gene Ontology (GO) enrichment analysis of transcripts that were differentially expressed (DE) between dpi across both genotypes revealed an inflammatory response for all dpi when compared to day 0. However, at the early time points of 4 and 7dpi, several GO terms had higher enrichment scores compared to later dpi, including inflammatory response (p < 10(-7)), specifically regulation of NFkappaB (p < 0.01), cytokine, and chemokine activity (p < 0.01). At 10 and 14dpi, GO term enrichment indicated a switch to DNA damage response, cell cycle checkpoints, and DNA replication. Few transcripts were DE between WUR genotypes on individual dpi or averaged over all dpi, and little enrichment of any GO term was found. However, there were differences in expression patterns over time between AA and AB animals, which was confirmed by genotype-specific expression patterns of several modules that were identified in weighted gene co-expression network analyses (WGCNA). Minor differences between AA and AB animals were observed in immune response and DNA damage response (p = 0.64 and p = 0.11, respectively), but a significant effect between genotypes pointed to a difference in ion transport/homeostasis and the participation of G-coupled protein receptors (p = 8e-4), which was reinforced by results from regulatory and phenotypic impact factor analyses between genotypes. Conclusion: We propose these pathway differences between WUR genotypes are the result of the inability of the truncated GBP5 of the AA genotyped pigs to inhibit viral entry and replication as quickly as the intact GBP5 protein of the AB genotyped pigs. C1 [Schroyen, Martine; Fritz-Waters, Eric; Reecy, James M.; Dekkers, Jack C. M.; Tuggle, Christopher K.] Iowa State Univ, Dept Anim Sci, 2255 Kildee Hall, Ames, IA 50011 USA. [Eisley, Christopher] Iowa State Univ, Dept Stat, 1121 Snedecor Hall, Ames, IA 50011 USA. [Koltes, James E.] Univ Arkansas, Dept Anim Sci, AFLS B106D, Fayetteville, AR 72701 USA. [Choi, Igseo; Lunney, Joan K.] USDA ARS, Beltsville Agr Res Ctr, APDL, Bldg 1040, Beltsville, MD 20705 USA. [Plastow, Graham S.; Guan, Leluo; Stothard, Paul; Bao, Hua; Kommadath, Arun] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. [Rowland, Robert R. R.] Kansas State Univ, Coll Vet Med, K-231 Mosier Hall, Manhattan, KS 66506 USA. RP Tuggle, CK (reprint author), Iowa State Univ, Dept Anim Sci, 2255 Kildee Hall, Ames, IA 50011 USA. EM cktuggle@iastate.edu FU USDA NIFA PRRS CAP Award [2008-55620-19132]; USDA NIFA award [2012-38420-19286]; Genome Canada; Genome Alberta; Alberta Livestock and Meat Agency; PigGen Canada; National Pork Board; NRSP-8 Swine Genome and Bioinformatics Coordination projects FX The authors acknowledge partial funding for this project provided by USDA NIFA PRRS CAP Award 2008-55620-19132, USDA NIFA award 2012-38420-19286, Genome Canada, Genome Alberta, the Alberta Livestock and Meat Agency and PigGen Canada. The samples for the RNA-seq analysis were provided by the PRRS Host Genetics Consortium (PHGC), which has been supported by the USDA NIFA PRRS CAP Award 2008-55620-19132, the National Pork Board, the NRSP-8 Swine Genome and Bioinformatics Coordination projects, and breeding companies associated with the PHGC, including Choice Genetics, Fast Genetics, Genesus, PIC/Genus, and Topigs. While members of the PRRS Host Genetics Consortium played important roles in the development of the original design for the PHGC studies, none of these funders had a role in the collection, analysis, and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication. NR 82 TC 0 Z9 0 U1 5 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 8 PY 2016 VL 17 AR 196 DI 10.1186/s12864-016-2547-z PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DF8GO UT WOS:000371595700001 PM 26951612 ER PT J AU Beckstead, J Meyer, SE Ishizuka, TS McEvoy, KM Coleman, CE AF Beckstead, Julie Meyer, Susan E. Ishizuka, Toby S. McEvoy, Kelsey M. Coleman, Craig E. TI Lack of Host Specialization on Winter Annual Grasses in the Fungal Seed Bank Pathogen Pyrenophora semeniperda SO PLOS ONE LA English DT Article ID WESTERN NORTH-AMERICA; BROMUS-TECTORUM; RED-QUEEN; POPULATION; RANGE; SPECIFICITY; GERMINATION; MORTALITY; DYNAMICS; GENETICS AB Generalist plant pathogens may have wide host ranges, but many exhibit varying degrees of host specialization, with multiple pathogen races that have narrower host ranges. These races are often genetically distinct, with each race causing highest disease incidence on its host of origin. We examined host specialization in the seed pathogen Pyrenophora semeniperda by reciprocally inoculating pathogen strains from Bromus tectorum and from four other winter annual grass weeds (Bromus diandrus, Bromus rubens, Bromus arvensis and Taeniatherum caput-medusae) onto dormant seeds of B. tectorum and each alternate host. We found that host species varied in resistance and pathogen strains varied in aggressiveness, but there was no evidence for host specialization. Most variation in aggressiveness was among strains within populations and was expressed similarly on both hosts, resulting in a positive correlation between strain-level disease incidence on B. tectorum and on the alternate host. In spite of this lack of host specialization, we detected weak but significant population genetic structure as a function of host species using two neutral marker systems that yielded similar results. This genetic structure is most likely due to founder effects, as the pathogen is known to be dispersed with host seeds. All host species were highly susceptible to their own pathogen races. Tolerance to infection (i.e., the ability to germinate even when infected and thereby avoid seed mortality) increased as a function of seed germination rate, which in turn increased as dormancy was lost. Pyrenophora semeniperda apparently does not require host specialization to fully exploit these winter annual grass species, which share many life history features that make them ideal hosts for this pathogen. C1 [Beckstead, Julie; Ishizuka, Toby S.; McEvoy, Kelsey M.] Gonzaga Univ, Dept Biol, Spokane, WA 99258 USA. [Meyer, Susan E.] USDA, Shrub Sci Lab, Forest Serv, Rocky Mt Res Stn, Provo, UT 84606 USA. [Coleman, Craig E.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. RP Meyer, SE (reprint author), USDA, Shrub Sci Lab, Forest Serv, Rocky Mt Res Stn, Provo, UT 84606 USA. EM semeyer@xmission.com FU Joint Fire Sciences Program [JFSP-2011-S-2-6] FX Joint Fire Sciences Program: www.firescience.gov/ (JFSP-2011-S-2-6; S.E.M., J.B.). Howard Hughes Medical Institute Undergraduate Science Education Program: http://www.hhmi.org/programs/science-education-research-training (J.B.). Claire and Robert McDonald Work Award Program at Gonzaga University (J.B.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 0 Z9 0 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 7 PY 2016 VL 11 IS 3 AR e0151058 DI 10.1371/journal.pone.0151058 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG3SL UT WOS:000371990100086 PM 26950931 ER PT J AU Demmer, E Van Loan, MD Rivera, N Rogers, TS Gertz, ER German, JB Smilowitz, JT Zivkovic, AM AF Demmer, Elieke Van Loan, Marta D. Rivera, Nancy Rogers, Tara S. Gertz, Erik R. German, J. Bruce Smilowitz, Jennifer T. Zivkovic, Angela M. TI Addition of a dairy fraction rich in milk fat globule membrane to a high-saturated fat meal reduces the postprandial insulinaemic and inflammatory response in overweight and obese adults SO JOURNAL OF NUTRITIONAL SCIENCE LA English DT Article DE Milk fat globule membrane: Postprandial inflammation: Saturated fat: Insulin: C-reactive protein: CVD: Cytokines ID INTERCELLULAR-ADHESION MOLECULE-1; METABOLIC SYNDROME; CARDIOVASCULAR-DISEASE; BOVINE-MILK; HEALTHY; MARKERS; PROTEIN; ACIDS; MEN; LIPOPOLYSACCHARIDE AB Meals high in SFA, particularly palmitate, are associated with postprandial inflammation and insulin resistance. Milk fat globule membrane (MFGM) has anti-inflammatory properties that may attenuate the negative effects of SFA-rich meals. Our objective was to examine the postprandial metabolic and inflammatory response to a high-fat meal composed of palm oil (PO) compared with PO with an added dairy fraction rich in MFGM (PO+MFGM) in overweight and obese men and women (n 36) in a randomised, double-blinded, cross-over trial. Participants consumed two isoenergetic high-fat meals composed of a smoothie enriched with PO with v. without a cream-derived complex milk lipid fraction (dairy fraction rich in MFGM) separated by a washout of 1-2 weeks. Serum cytokines, adhesion molecules, cortisol and markers of inflammation were measured at fasting, and at 1, 3 and 6 h postprandially. Glucose, insulin and lipid profiles were analysed in plasma. Consumption of the PO + MFGM v. PO meal resulted in lower total cholesterol (P = 0.021), LDL-cholesterol (P = 0.046), soluble intracellular adhesion molecule (P = 0.005) and insulin (P = 0.005) incremental AUC, and increased IL-10 (P = 0.013). Individuals with high baseline C-reactive protein (CRP) concentrations (= 3 mg/l, n 17) had higher (P = 0.030) insulin at 1 h after the PO meal than individuals with CRP concentrations <3 mg/l (n 19). The addition of MFGM attenuated this difference between CRP groups. The addition of a dairy fraction rich in MFGM attenuated the negative effects of a high-SFA meal by reducing postprandial cholesterol, inflammatory markers and insulin response in overweight and obese individuals, particularly in those with elevated CRP. C1 [Demmer, Elieke; Van Loan, Marta D.; Rivera, Nancy; Rogers, Tara S.; Zivkovic, Angela M.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Van Loan, Marta D.; Gertz, Erik R.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [German, J. Bruce; Smilowitz, Jennifer T.; Zivkovic, Angela M.] Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA. [German, J. Bruce; Smilowitz, Jennifer T.] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. RP Zivkovic, AM (reprint author), Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA.; Zivkovic, AM (reprint author), Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA. EM amzivkovic@ucdavis.edu NR 53 TC 0 Z9 0 U1 6 U2 11 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 2048-6790 J9 J NUTR SCI JI J. Nutr. Sci. PD MAR 7 PY 2016 VL 5 AR e14 DI 10.1017/jns.2015.42 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DG1DR UT WOS:000371806200001 PM 27313850 ER PT J AU Clark, AJ Sarti-Dvorjak, D Brown-Guedira, G Dong, Y Baik, BK Van Sanford, DA AF Clark, Anthony J. Sarti-Dvorjak, Daniela Brown-Guedira, Gina Dong, Yanhong Baik, Byung-Kee Van Sanford, David A. TI Identifying Rare FHB-Resistant Segregants in Intransigent Backcross and F-2 Winter Wheat Populations SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE resistance breeding; Fusarium head blight; deoxynivalenol; soft red winter wheat; marker-assisted selection ID FUSARIUM-HEAD-BLIGHT; SPRING WHEAT; QTL ANALYSIS; DEOXYNIVALENOL; SCAB; SELECTION; BARLEY; HERITABILITY AB Fusarium head blight (FHB), caused mainly by Fusarium graminearum Schwabe [telomorph: Gibberella zeae Schwein.(Petch)] in the US, is one of the most destructive diseases of wheat (Triticum aestivem L. and T durum L.). Infected grain is usually contaminated with deoxynivalenol (DON), a serious mycotoxin. The challenge in FHB resistance breeding is combining resistance with superior agronomic and quality characteristics. Exotic QTL are widely used to improve FHB resistance. Success depends on the genetic background into which the OIL are introgressed, whether through backcrossing or forward crossing; QTL expression is impossible to predict. In this study four high-yielding soft red winter wheat breeding lines with little or no scab resistance were each crossed to a donor parent (VA01W-476) with resistance alleles at two QTL: Fhb1 (chromosome 3BS) and QFhs,nau-2DL (chromosome 2DL) to generate backcross and F-2 progeny. F-2 individuals were genotyped and assigned to 4 groups according to presence/ absence of resistance alleles at one or both QTL. The effectiveness of these QTL in reducing FHB rating, incidence, index, severity, Fusariurn-damaged kernels (FDK) and DON, in F-2-derived lines was assessed over 2 years. Fhbl showed an average reduction in DON of 17.5%, and conferred significant resistance in 3 of 4 populations. QFhs.nau-2DL reduced DON 6.7% on average and conferred significant resistance in 2 of 4 populations. The combination of Fhbl and OFhs.nau-2DL resistance reduced DON 25.5% across all populations. Double resistant lines had significantly reduced DON compared to double susceptible lines in 3 populations. Backcross derived progeny were planted in replicated yield trials (2011 and 2012) and in a scab nursery in 2012. Several top yielding lines performed well in the scab nursery, with acceptable DON concentrations, even though the average effect of either QTL in this population was not significant. Population selection is often viewed as an "all or nothing" process: if the average resistance level is insufficient, the population is discarded. These results indicate that it may be possible to find rare segregants which combine scab resistance, superior agronomic performance and acceptable quality even in populations in which the average effect of the QTL is muted or negligible. C1 [Clark, Anthony J.; Van Sanford, David A.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY USA. [Sarti-Dvorjak, Daniela] Syngenta Seeds, Des Moines, IA USA. [Brown-Guedira, Gina] ARS, Plant Sci Res Unit, USDA, Raleigh, NC USA. [Dong, Yanhong] Univ Minnesota, Dept Plant Pathol, St Paul, MN USA. [Baik, Byung-Kee] ARS, Soft Wheat Qual Lab, USDA, Wooster, OH USA. RP Van Sanford, DA (reprint author), Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY USA. EM dvs@uky.eou FU U.S. Department of Agriculture [59-020-69-054] FX DS conducted this research in partial fulfillment of the requirements to obtain a PhD degree at the University of Kentucky. This material is based on work supported by the U.S. Department of Agriculture, under Agreement No. 59-020-69-054. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. We thank John Connelley and Sandy Swanson for their technical assistance. NR 34 TC 1 Z9 1 U1 4 U2 16 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAR 7 PY 2016 VL 7 AR 277 DI 10.3389/fmicb.2016.00277 PG 14 WC Microbiology SC Microbiology GA DF5GC UT WOS:000371378800002 PM 27014202 ER PT J AU Die, JV Roman, B Flores, F Rowland, LJ AF Die, Jose V. Roman, Belen Flores, Fernando Rowland, Lisa J. TI Design and Sampling Plan Optimization for RT-qPCR Experiments in Plants: A Case Study in Blueberry SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE blueberry; confounding variation; qPCR; replicates; RT variability ID GENE-EXPRESSION ANALYSIS; REAL-TIME PCR; COLD-ACCLIMATION; QUANTITATIVE PCR; SYSTEMATIC VALIDATION; RNA QUALITY; QUANTIFICATION; REFERENCES; VARIANCE; PATTERNS AB The qPCR assay has become a routine technology in plant biotechnology and agricultural research. It is unlikely to be technically improved, but there are still challenges which center around minimizing the variability in results and transparency when reporting technical data in support of the conclusions of a study. There are a number of aspects of the pre- and post-assay workflow that contribute to variability of results. Here, through the study of the introduction of error in qPCR measurements at different stages of the workflow, we describe the most important causes of technical variability in a case study using blueberry. In this study, we found that the stage for which increasing the number of replicates would be the most beneficial depends on the tissue used. For example, we would recommend the use of more RT replicates when working with leaf tissue, while the use of more sampling (RNA extraction) replicates would be recommended when working with stems or fruits to obtain the most optimal results. The use of more qPCR replicates provides the least benefit as it is the most reproducible step. By knowing the distribution of error over an entire experiment and the costs at each step, we have developed a script to identify the optimal sampling plan within the limits of a given budget. These findings should help plant scientists improve the design of qPCR experiments and refine their laboratory practices in order to conduct qPCR assays in a more reliable-manner to produce more consistent and reproducible data. C1 [Die, Jose V.; Rowland, Lisa J.] USDA ARS, Beltsville, MD USA. [Roman, Belen] IFAPA Ctr Alameda Obispo, Area Mejora & Biotecnol, Cordoba, Spain. [Flores, Fernando] Univ Huelva, Dept Ciencias Agroforest, Huelva, Spain. RP Die, JV (reprint author), USDA ARS, Beltsville, MD USA. EM jose.die@ars.usda.cov OI Flores Gil, Fernando/0000-0002-5247-2267; Die, Jose V./0000-0002-7506-8590 NR 48 TC 0 Z9 0 U1 6 U2 22 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 7 PY 2016 VL 7 AR 271 DI 10.3389/fpls.2016.00271 PG 9 WC Plant Sciences SC Plant Sciences GA DF5NS UT WOS:000371399400001 PM 27014296 ER PT J AU Yang, LM Ji, JH Wang, HL Harris-Shultz, KR Abd Allah, EF Luo, YM Guan, YL Hu, XY AF Yang, Liming Ji, Jianhui Wang, Hongliang Harris-Shultz, Karen R. Abd Allah, Elsayed F. Luo, Yuming Guan, Yanlong Hu, Xiangyang TI Carbon Monoxide Interacts with Auxin and Nitric Oxide to Cope with Iron Deficiency in Arabidopsis SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE CO; NO; auxin; iron deficiency; Arabidopsis; basipetal transport ID TRANSCRIPTION FACTOR FIT; REACTIVE OXYGEN; OXIDATIVE DAMAGE; HEME OXYGENASES; UP-REGULATION; ROOTS; HOMEOSTASIS; PLANTS; GENE; BIOSYNTHESIS AB To clarify the roles of carbon monoxide (CO), nitric oxide (NO), and auxin in the plant response to iron deficiency (-Fe), and to establish how the signaling molecules interact to enhance Fe acquisition, we conducted physiological, genetic, and molecular analyses that compared the responses of various Arabidopsis mutants, including hy1 (CO deficient), noa1 (NO deficient), nia1/nia2 (NO deficient), yuc1 (auxin over-accumulation), and cue1 (NO over-accumulation) to-Fe stress. We also generated a HY1 over expression line (named HY1-OX) in which CO is over-produced compared to wild-type. We found that the suppression of CO and NO generation using various inhibitors enhanced the sensitivity of wild-type plants to Fe depletion. Similarly, the hy1, noa1, and nia1/nia2 mutants were more sensitive to Fe deficiency. By contrast, the yuc1, cuel, and HY1-OX lines were less sensitive to Fe depletion. The hy1 mutant with low CO content exhibited no induced expression of the Fe uptake related genes FIT1 and FRO2 as compared to wild-type plants. On the other hand, the treatments of exogenous CO and NO enhanced Fe uptake. Likewise, cuel and HY1-OX lines with increased endogenous content of NO and CO, respectively, also exhibited enhanced Fe uptake and increased expression of bHLH transcriptional factor FIT1 as compared to wild-type plants. Furthermore, we found that CO affected auxin accumulation and transport in the root tip by altering the PIN1 and PIN2 proteins distribution that control lateral root structure under-Fe stress. Our results demonstrated the integration of CO, NO, and auxin signaling to cope with Fe deficiency in Arabidopsis. C1 [Yang, Liming; Ji, Jianhui; Luo, Yuming] Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr &, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Huaian, Peoples R China. [Yang, Liming] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Yang, Liming] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Wang, Hongliang; Harris-Shultz, Karen R.] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Abd Allah, Elsayed F.] King Saud Univ, Coll Food & Agr Sci, Dept Plant Prod, Riyadh, Saudi Arabia. [Guan, Yanlong; Hu, Xiangyang] Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China. RP Guan, YL; Hu, XY (reprint author), Chinese Acad Sci, Inst Tibet Plateau Res Kunming, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming, Peoples R China. EM guanyanlong@mail.kib.ac.cn; huxiangyang@mail.kib.ac.cn FU National High-tech R&D Program of China [2013AA102705]; National Science Foundation grant of China [30900871, 30971452, 31170256, 31400169]; Natural Science Foundation of Jiangsu Province [BK2011409, BK20140454]; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environment Protection [HSXT305]; Major Science and Technology Program [110201101003-TS-03, TS-02-20110014, 2011YN02, 2011YN03]; Jiangsu Government Scholarship for Overseas Studies; Qinglan Project of Jiangsu Province; Deanship of Scientific Research at King Saud University [RG- 1435-014] FX This work was supported by National High-tech R&D Program of China (2013AA102705), National Science Foundation grant of China (30900871, 30971452, 31170256, 31400169), Natural Science Foundation of Jiangsu Province (BK2011409, BK20140454), Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environment Protection (HSXT305), Major Science and Technology Program (110201101003-TS-03, TS-02-20110014, 2011YN02, and 2011YN03), Jiangsu Government Scholarship for Overseas Studies and Qinglan Project of Jiangsu Province. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding this Research group NO (RG- 1435-014). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 47 TC 0 Z9 0 U1 6 U2 19 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAR 7 PY 2016 VL 7 AR 112 DI 10.3389/fpls.2016.00112 PG 15 WC Plant Sciences SC Plant Sciences GA DF5NM UT WOS:000371398800001 PM 27014280 ER PT J AU Trost, B Stuber, T Surujballi, O Nelson, J Robbe-Austerman, S Smith, NH Desautels, L Tikoo, SK Griebel, P AF Trost, Brett Stuber, Tod Surujballi, Om Nelson, Jeffrey Robbe-Austerman, Suelee Smith, Noel H. Desautels, Louis Tikoo, Suresh K. Griebel, Philip TI Investigation of the cause of geographic disparities in IDEXX ELISA sensitivity in serum samples from Mycobacterium bovis-infected cattle SO SCIENTIFIC REPORTS LA English DT Article ID MULTIPLE SEQUENCE ALIGNMENT; LINKED-IMMUNOSORBENT-ASSAY; TUBERCULOSIS; MPB70; GENERATION; RESOURCE; DATABASE; SEARCH; MPB83; SIGK AB Accurately identifying Mycobacterium bovis-infected cattle is critical for bovine tuberculosis prevention and control. One method for identifying infected cattle is an ELISA developed by IDEXX laboratories, which detects antibodies to two M. bovis proteins, MPB70 and MPB83. The assay's sensitivity varies by geographic region, with sensitivities of 77%, 45%, and 9% in bovine serum samples from the United Kingdom (n=126), the United States (n=146), and Mexico (n=128), respectively. We hypothesized that geographically-biased sequence variation in mpb70 and mpb83, or in the genes that regulate their expression (sigK and rskA), may explain these differing sensitivities. This hypothesis was tested by comparing the sequences of these four genes in 455 M. bovis strains isolated from cattle in the aforementioned countries. For each gene, a single, common sequence was identified in most genomes of the M. bovis strains collected in all three countries. Twelve of the 455 strains were isolated from infected cattle for which the IDEXX ELISA was also performed. Five of the seven ELISA-positive genomes and three of the five ELISA-negative genomes contained the most common sequence of all four genes. Thus, sequence variation in mpb70, mpb83, sigK, and rskA does not explain the geographic disparities in IDEXX ELISA sensitivity. C1 [Trost, Brett; Tikoo, Suresh K.; Griebel, Philip] Univ Saskatchewan, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada. [Trost, Brett] Univ Saskatchewan, Dept Comp Sci, Saskatoon, SK S7N 0W0, Canada. [Stuber, Tod; Nelson, Jeffrey; Robbe-Austerman, Suelee] Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, USDA, Ames, IA 50010 USA. [Surujballi, Om] Canadian Food Inspect Agcy, Ottawa, ON K2H 8P9, Canada. [Smith, Noel H.] Anim & Plant Hlth Agcy, New Haw KT15 3NB, Surrey, England. [Desautels, Louis] Canadian Cattlemens Assoc, Calgary, AB T2E 7H7, Canada. [Tikoo, Suresh K.; Griebel, Philip] Univ Saskatchewan, Sch Publ Hlth, Vaccinol & Immunotherapeut Program, Saskatoon, SK S7N 5E3, Canada. RP Griebel, P (reprint author), Univ Saskatchewan, Vaccine & Infect Dis Org, Saskatoon, SK S7N 5E3, Canada.; Griebel, P (reprint author), Univ Saskatchewan, Sch Publ Hlth, Vaccinol & Immunotherapeut Program, Saskatoon, SK S7N 5E3, Canada. EM philip.griebel@usask.ca RI APHA, Staff publications/E-6082-2010; Trost, Brett/K-4127-2016 OI Trost, Brett/0000-0003-4863-7273 FU Alberta Livestock and Meat Agency (ALMA); Manitoba Beef Producers (MBP); British Columbia Cattlemen's Association (BCCA) Horn Levy Fund; Dairy Farmers of Canada (DFC); Canadian Cattlemen's Association (CCA); Tier I Canada Research Chair (CRC) in Neonatal Mucosal Immunology by the Canadian Institutes of Health Research (CIHR); Defra project [SB4020] FX The authors thank Dr. Javier Nunez (APHA) for assisting with the procurement of M. bovis sequence data. For their participation and cooperation in this study, and/or for providing serum samples or M. bovis genome sequences, we thank the United States Department of Agriculture (USDA); the Canadian Food Inspection Agency (CFIA); the Mexico Secretariat of Agriculture, Livestock, Rural Development, Fisheries and Food (SAGARPA); and the United Kingdom's Animal Health and Veterinary Laboratory Agency (AHVLA/UK). All infected animals used in this study were in well-identified herds in quarantine, with all proper animal health precautions having been taken. Funding was provided by Alberta Livestock and Meat Agency (ALMA), Manitoba Beef Producers (MBP), British Columbia Cattlemen's Association (BCCA) Horn Levy Fund, Dairy Farmers of Canada (DFC), and Canadian Cattlemen's Association (CCA). P.G. is funded by a Tier I Canada Research Chair (CRC) in Neonatal Mucosal Immunology provided by the Canadian Institutes of Health Research (CIHR). N.H.S. was funded by Defra project SB4020. NR 36 TC 1 Z9 1 U1 0 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 7 PY 2016 VL 6 AR 22763 DI 10.1038/srep22763 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF6KE UT WOS:000371463600003 PM 26949166 ER PT J AU Williams, AH Sharma, M Thatcher, LF Azam, S Hane, JK Sperschneider, J Kidd, BN Anderson, JP Ghosh, R Garg, G Lichtenzveig, J Kistler, HC Shea, T Young, S Buck, SAG Kamphuis, LG Saxena, R Pande, S Ma, LJ Varshney, RK Singh, KB AF Williams, Angela H. Sharma, Mamta Thatcher, Louise F. Azam, Sarwar Hane, James K. Sperschneider, Jana Kidd, Brendan N. Anderson, Jonathan P. Ghosh, Raju Garg, Gagan Lichtenzveig, Judith Kistler, H. Corby Shea, Terrance Young, Sarah Buck, Sally-Anne G. Kamphuis, Lars G. Saxena, Rachit Pande, Suresh Ma, Li-Jun Varshney, Rajeev K. Singh, Karam B. TI Comparative genomics and prediction of conditionally dispensable sequences in legume-infecting Fusarium oxysporum formae speciales facilitates identification of candidate effectors SO BMC GENOMICS LA English DT Article DE Fusarium; Conditionally dispensable chromosomes (CDC); Effectors; Pathogenicity; Legume; Pulse; Fungal pathogen ID HIDDEN MARKOV MODEL; F-SP CICERIS; PATHOGEN NECTRIA-HAEMATOCOCCA; HORIZONTAL GENE-TRANSFER; FUNGAL PLANT-PATHOGENS; PHYTOALEXINS MEDICARPIN; DISEASE RESISTANCE; GARDEN PEA; SP CUBENSE; PHI-BASE AB Background: Soil-borne fungi of the Fusarium oxysporum species complex cause devastating wilt disease on many crops including legumes that supply human dietary protein needs across many parts of the globe. We present and compare draft genome assemblies for three legume-infecting formae speciales (ff. spp.): F. oxysporum f. sp. ciceris (Foc-38-1) and f. sp. pisi (Fop-37622), significant pathogens of chickpea and pea respectively, the world's second and third most important grain legumes, and lastly f. sp. medicaginis (Fom-5190a) for which we developed a model legume pathosystem utilising Medicago truncatula. Results: Focusing on the identification of pathogenicity gene content, we leveraged the reference genomes of Fusarium pathogens F. oxysporum f. sp. lycopersici (tomato-infecting) and F. solani (pea-infecting) and their well-characterised core and dispensable chromosomes to predict genomic organisation in the newly sequenced legume-infecting isolates. Dispensable chromosomes are not essential for growth and in Fusarium species are known to be enriched in host-specificity and pathogenicity-associated genes. Comparative genomics of the publicly available Fusarium species revealed differential patterns of sequence conservation across F. oxysporum formae speciales, with legume-pathogenic formae speciales not exhibiting greater sequence conservation between them relative to non-legume-infecting formae speciales, possibly indicating the lack of a common ancestral source for legume pathogenicity. Combining predicted dispensable gene content with in planta expression in the model legume-infecting isolate, we identified small conserved regions and candidate effectors, four of which shared greatest similarity to proteins from another legume-infecting ff. spp. Conclusions: We demonstrate that distinction of core and potential dispensable genomic regions of novel F. oxysporum genomes is an effective tool to facilitate effector discovery and the identification of gene content possibly linked to host specificity. While the legume-infecting isolates didn't share large genomic regions of pathogenicity-related content, smaller regions and candidate effector proteins were highly conserved, suggesting that they may play specific roles in inducing disease on legume hosts. C1 [Ma, Li-Jun] Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA. [Williams, Angela H.; Anderson, Jonathan P.; Kamphuis, Lars G.; Varshney, Rajeev K.; Singh, Karam B.] Univ Western Australia, Inst Agr, 35 Stirling Highway, Crawley, WA 6009, Australia. [Williams, Angela H.; Thatcher, Louise F.; Hane, James K.; Sperschneider, Jana; Kidd, Brendan N.; Anderson, Jonathan P.; Garg, Gagan; Buck, Sally-Anne G.; Kamphuis, Lars G.; Singh, Karam B.] CSIRO Agr, Ctr Environm & Life Sci, Wembley, WA 6913, Australia. [Sharma, Mamta; Azam, Sarwar; Ghosh, Raju; Saxena, Rachit; Pande, Suresh; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Greater Hyderabad 502324, Telangana, India. [Hane, James K.] Curtin Univ, Ctr Crop & Dis Management, Curtin Inst Computat, Dept Environm & Agr, Perth, WA 6102, Australia. [Hane, James K.] Curtin Univ, Ctr Crop & Dis Management, CCDM Bioinformat, Perth, WA 6102, Australia. [Lichtenzveig, Judith] Curtin Univ, Ctr Crop & Dis Management, Dept Environm & Agr, Pulse Pathol & Genet, Perth, WA 6102, Australia. [Lichtenzveig, Judith] Curtin Univ, Curtin Inst Computat, Perth, WA 6102, Australia. [Kistler, H. Corby] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Shea, Terrance; Young, Sarah] Broad Inst, Cambridge, MA 02141 USA. RP Ma, LJ (reprint author), Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA.; Varshney, RK; Singh, KB (reprint author), Univ Western Australia, Inst Agr, 35 Stirling Highway, Crawley, WA 6009, Australia. EM lijun@biochem.umass.edu; R.K.Varshney@CGIAR.ORG; Karam.Singh@csiro.au RI Singh, Karam/C-3235-2012; Anderson, Jonathan/A-7244-2008; Thatcher, Louise/A-1302-2010; Kidd, Brendan/J-9672-2014; OI Singh, Karam/0000-0002-2777-7448; Anderson, Jonathan/0000-0002-4783-5393; Kidd, Brendan/0000-0003-1770-1404; Sperschneider, Jana/0000-0002-9385-8588 NR 140 TC 2 Z9 2 U1 13 U2 32 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD MAR 5 PY 2016 VL 17 AR 191 DI 10.1186/s12864-016-2486-8 PG 24 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DF8GK UT WOS:000371595300006 PM 26945779 ER PT J AU Bearson, BL Bearson, SMD Kich, JD AF Bearson, Bradley L. Bearson, Shawn M. D. Kich, Jalusa D. TI A DIVA vaccine for cross-protection against Salmonella SO VACCINE LA English DT Article DE Salmonella; Swine; Vaccine; DIVA; Cross-protection ID ENTERICA SEROVAR TYPHIMURIUM; SENSOR KINASE; NONCODING RNA; SWINE; NOREPINEPHRINE; COLONIZATION; MOTILITY; EFFICACY; IMMUNITY; FARMS AB Swine are often asymptomatic carriers of Salmonella spp., a leading cause of human bacterial food borne disease. Vaccination against Salmonella is effective for protecting animal health and enhancing food safety. However, with >2500 Salmonella serovars, current vaccines for swine offer limited cross protection against heterologous serovars. Also, existing vaccines can interfere with surveillance programs that monitor the Salmonella status of swine herds. To overcome Salmonella vaccine limitations, we rationally designed and constructed an attenuated Salmonella enterica serovar Typhimurium vaccine (BBS 866) by deleting multiple small regulatory RNA (sRNA) genes (omrA, omrB, rybB, micA, and invR) in combination with an rfaH mutation. We vaccinated swine intranasally at 3-weeks of age with PBS (mock-vaccinated), BBS 866 or BBS 202 (S. Typhimurium rfaH, Bearson et al., Front Vet Sci 2014;1:9.) and challenged at 7-weeks of age with virulent S. Choleraesuis, a swine pathogen. Vaccination with BBS 866 enhanced protection against S. Choleraesuis by significantly limiting the duration of fever, weight loss, the levels of circulating INF gamma, and the total number of swine with S. Choleraesuis septicemia. Vaccination with either BBS 866 or BBS 202 significantly reduced S. Choleraesuis colonization of both systemic (spleen and liver) and gastrointestinal (Peyer's Patch, Ileocecal lymph nodes, and cecum) tissues. Similar to our earlier report for BBS 202, the BBS 866 vaccine strain can be used in swine without compromising the differentiation of infected from vaccinated animals (DIVA). Therefore, the attenuated S. Typhimurium BBS 866 strain, containing mutations in rfaH and multiple sRNAs, addresses the limitations of current Salmonella vaccines by providing cross-protection against Salmonella serovars in swine without interfering with established monitoring programs for Salmonella surveillance. Published by Elsevier Ltd. C1 [Bearson, Bradley L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Bearson, Shawn M. D.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. [Kich, Jalusa D.] Embrapa Swine & Poultry, Concordia, SC, Brazil. RP Bearson, BL (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM brad.bearson@ars.usda.gov FU USDA, ARS CRIS funds FX We are greatly appreciative of the outstanding technical support of Kellie Winter, Jennifer Jones, and Stephanie Jones. This research was supported by USDA, ARS CRIS funds. NR 20 TC 2 Z9 2 U1 1 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD MAR 4 PY 2016 VL 34 IS 10 BP 1241 EP 1246 DI 10.1016/j.vaccine.2016.01.036 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DG3DO UT WOS:000371949900002 PM 26836212 ER PT J AU McGill, JL Rusk, RA Guerra-Maupome, M Briggs, RE Sacco, RE AF McGill, Jodi L. Rusk, Rachel A. Guerra-Maupome, Mariana Briggs, Robert E. Sacco, Randy E. TI Bovine Gamma Delta T Cells Contribute to Exacerbated IL-17 Production in Response to Co-Infection with Bovine RSV and Mannheimia haemolytica SO PLOS ONE LA English DT Article ID RESPIRATORY SYNCYTIAL VIRUS; VIRAL-INFECTION; MYCOBACTERIUM-BOVIS; CYTOKINE PRODUCTION; ANIMAL-MODELS; WILD-TYPE; DISEASE; EXPRESSION; INFANTS; CALVES AB Human respiratory syncytial virus (HRSV) is a leading cause of severe lower respiratory tract infection in children under five years of age. IL-17 and Th17 responses are increased in children infected with HRSV and have been implicated in both protective and pathogenic roles during infection. Bovine RSV (BRSV) is genetically closely related to HRSV and is a leading cause of severe respiratory infections in young cattle. While BRSV infection in the calf parallels many aspects of human infection with HRSV, IL-17 and Th17 responses have not been studied in the bovine. Here we demonstrate that calves infected with BRSV express significant levels of IL-17, IL-21 and IL-22; and both CD4 T cells and Upsilon delta T cells contribute to this response. In addition to causing significant morbidity from uncomplicated infections, BRSV infection also contributes to the development of bovine respiratory disease complex (BRDC), a leading cause of morbidity in both beef and dairy cattle. BRDC is caused by a primary viral infection, followed by secondary bacterial pneumonia by pathogens such as Mannheimia haemolytica. Here, we demonstrate that in vivo infection with M. haemolytica results in increased expression of IL-17, IL-21 and IL-22. We have also developed an in vitro model of BRDC and show that co-infection of PBMC with BRSV followed by M. haemolytica leads to significantly exacerbated IL-17 production, which is primarily mediated by IL-17-producing Upsilon delta T cells. Together, our results demonstrate that calves, like humans, mount a robust IL-17 response during RSV infection; and suggest a previously unrecognized role for IL-17 and Upsilon delta T cells in the pathogenesis of BRDC. C1 [McGill, Jodi L.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Rusk, Rachel A.; Guerra-Maupome, Mariana] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Pathobiol Grad Program, Manhattan, KS 66506 USA. [Briggs, Robert E.; Sacco, Randy E.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, Ames, IA USA. RP McGill, JL (reprint author), Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. EM jlmcgill@vet.k-state.edu FU USDA; Kansas State University FX A portion of this research was conducted at a USDA research facility and funding was provided through internal USDA research dollars. This research was also funded through start-up funds to Dr. McGill provided by Kansas State University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 63 TC 1 Z9 1 U1 4 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 4 PY 2016 VL 11 IS 3 AR e0151083 DI 10.1371/journal.pone.0151083 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG0FG UT WOS:000371739400079 PM 26942409 ER PT J AU Latham, EA Anderson, RC Pinchak, WE Nisbet, DJ AF Latham, Elizabeth A. Anderson, Robin C. Pinchak, William E. Nisbet, David J. TI Insights on Alterations to the Rumen Ecosystem by Nitrate and Nitrocompounds SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE nitrate; nitrocompounds; rumen; methane reduction; nitrate toxicity ID RUMINAL METHANE PRODUCTION; NITROPROPANOL-METABOLIZING BACTERIUM; FATTY-ACID PRODUCTION; IN-VITRO; DIETARY NITRATE; NITROUS-OXIDE; DAIRY-COWS; SALMONELLA-TYPHIMURIUM; ESCHERICHIA-COLI; BEEF-CATTLE AB Nitrate and certain short chain nitrocompounds and nitro-oxy compounds are being investigated as dietary supplements to reduce economic and environmental costs associated with ruminal methane emissions. Thermodynamically, nitrate is a preferred electron acceptor in the rumen that consumes electrons at the expense of methanogenesis during dissimilatory reduction to an intermediate, nitrite, which is primarily reduced to ammonia although small quantities of nitrous oxide may also be produced. Short chain nitrocompounds act as direct inhibitors of methanogenic bacteria although certain of these compounds may also consume electrons at the expense of methanogenesis and are effective inhibitors of important foodborne pathogens. Microbial and nutritional consequences of incorporating nitrate into ruminant diets typically results in increased acetate production. Unlike most other methane-inhibiting supplements, nitrate decreases or has no effect on propionate production. The type of nitrate salt added influences rates of nitrate reduction, rates of nitrite accumulation and efficacy of methane reduction, with sodium and potassium salts being more potent than calcium nitrate salts. Digestive consequences of adding nitrocompounds to ruminant diets are more variable and may in some cases increase propionate production. Concerns about the toxicity of nitrate's intermediate product, nitrite, to ruminants necessitate management, as animal poisoning may occur via methemoglobinemia. Certain of the naturally occurring nitrocompounds, such as 3 -nitro-1-propionate or 3-nitro-1-propanol also cause poisoning but via inhibition of succinate dehydrogenase. Typical risk management procedures to avoid nitrite toxicity involve gradually adapting the animals to higher concentrations of nitrate and nitrite, which could possibly be used with the nitrocompounds as well. A number of organisms responsible for nitrate metabolism in the rumen have been characterized. To date a single rumen bacterium is identified as contributing appreciably to nitrocompound metabolism. Appropriate doses of the nitrocompounds and nitrate, singly or in combination with probiotic bacteria selected for nitrite and nitrocompound detoxification activity promise to alleviate risks of toxicity. Further studies are needed to more clearly define benefits and risk of these technologies to make them saleable for livestock producers. C1 [Latham, Elizabeth A.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Latham, Elizabeth A.; Pinchak, William E.] Texas A&M AgriLife Res, Vernon, TX USA. [Anderson, Robin C.; Nisbet, David J.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX USA. RP Anderson, RC (reprint author), ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX USA. EM robin.anderson@ars.usda.gov NR 125 TC 5 Z9 5 U1 13 U2 35 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAR 4 PY 2016 VL 7 AR 228 DI 10.3389/fmicb.2016.00228 PG 15 WC Microbiology SC Microbiology GA DF4XY UT WOS:000371356000001 PM 26973609 ER PT J AU Buss, BF Keyser-Metobo, A Rother, J Holtz, L Gall, K Jereb, J Murphy, CN Iwen, PC Robbe-Austerman, S Holcomb, MA Infield, P AF Buss, Bryan F. Keyser-Metobo, Alison Rother, Julie Holtz, Laura Gall, Kristin Jereb, John Murphy, Caitlin N. Iwen, Peter C. Robbe-Austerman, Suelee Holcomb, Melissa A. Infield, Pat TI Possible Airborne Person-to-Person Transmission of Mycobacterium bovis - Nebraska 2014-2015 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID UNITED-STATES; HUMAN TUBERCULOSIS C1 [Buss, Bryan F.] CDC, Career Epidemiol Field Officer Program, Div State & Local Readiness, Off Publ Hlth Preparedness & Response, Atlanta, GA 30333 USA. [Buss, Bryan F.; Keyser-Metobo, Alison; Gall, Kristin; Holcomb, Melissa A.; Infield, Pat] Nebraska Dept Hlth & Human Serv, Div Publ Hlth, Lincoln, NE USA. [Rother, Julie] Northeast Nebraska Publ Hlth Dept, Wayne, NE USA. [Holtz, Laura] Elkhorn Logan Valley Publ Hlth Dept, Wisner, NE USA. [Jereb, John] CDC, Field Serv & Evaluat Branch, Div TB Eliminat, Natl Ctr HIV AIDS Viral Hepatitis STD & TB Preven, Atlanta, GA 30333 USA. [Murphy, Caitlin N.; Iwen, Peter C.] Univ Nebraska Med Ctr, Dept Pathol & Microbiol, Lincoln, NE USA. [Iwen, Peter C.] Univ Nebraska Med Ctr, Nebraska Publ Hlth Lab, Lincoln, NE USA. [Robbe-Austerman, Suelee] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. RP Buss, BF (reprint author), CDC, Career Epidemiol Field Officer Program, Div State & Local Readiness, Off Publ Hlth Preparedness & Response, Atlanta, GA 30333 USA.; Buss, BF (reprint author), Nebraska Dept Hlth & Human Serv, Div Publ Hlth, Lincoln, NE USA. EM feu7@cdc.gov NR 10 TC 4 Z9 4 U1 1 U2 3 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD MAR 4 PY 2016 VL 65 IS 8 BP 197 EP 201 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DF4UC UT WOS:000371345500001 PM 26938831 ER PT J AU Devatkal, SK Jaiswal, P Kaur, A Juneja, V AF Devatkal, Suresh K. Jaiswal, Pranita Kaur, Amanpreet Juneja, Vijay TI Inactivation of Bacillus cereus and Salmonella enterica serovar typhimurium by Aqueous Ozone: Modeling and UV-Vis Spectroscopic Analysis SO OZONE-SCIENCE & ENGINEERING LA English DT Article DE Bacillus cereus; Biphasic Model; Double Weibull Model; Kinetic Modeling; Ozone Treatment; Principal Component Analysis; Salmonella typhimurium ID LISTERIA-MONOCYTOGENES; THERMAL INACTIVATION; VEGETATIVE CELLS; SURVIVAL CURVES; FOOD; KINETICS; SAFETY; SPORES AB Antibacterial efficacy of aqueous ozone (O-3) against B. cereus vegetative cells and S. typhimurium by was studied by using GInaFiT tool and results were validated using UV-Vis spectroscopy. Ozone gas was generated using a domestic ozone generator. Buffer solutions containing known amounts (approximate to 10(8)-10(9)cfu/mL) of bacterial pathogens were treated with aqueous ozone (200mg/hr or 0.1mg/l) for 16min with sampling at 0-, 0.5-, 1-, 2-, 4-, 8- and 16-min intervals. A reduction of 4.6 log of B. cereus and 7.7 log cycle reduction of S. typhimurium was obtained in 16min. Biphasic shoulder and double Weibull models were good fit for the experimental inactivation kinetics data. Principal Component Analysis showed discrete grouping based on the time of treatment. The highest correct classification results for SIMCA were achieved for both B. cereus and S. typhimurium after 1 and 8min of treatment, respectively. In partial least squares regression analysis, maximum R-2 values for calibration and validation were found to be 0.84, 0.80 for B. cereus and 0.90, 0.89 for S. typhimurium, respectively. C1 [Devatkal, Suresh K.] ICAR Res Complex, Natl Res Ctr Meat, Hyderabad 500092, TS, India. [Jaiswal, Pranita; Kaur, Amanpreet] ICAR Res Complex, Cent Inst Postharvest Engn & Technol, PAU Campus, Ludhiana 141004, Punjab, India. [Juneja, Vijay] USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Devatkal, SK (reprint author), 108 Natl Res Ctr Meat, Post Box 19 Boduppal Post, Hyderabad 500092, Andhra Pradesh, India. EM sureshlpt@yahoo.com NR 25 TC 0 Z9 0 U1 5 U2 12 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0191-9512 EI 1547-6545 J9 OZONE-SCI ENG JI Ozone-Sci. Eng. PD MAR 3 PY 2016 VL 38 IS 2 BP 124 EP 132 DI 10.1080/01919512.2015.1079119 PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DG4BZ UT WOS:000372016700006 ER PT J AU Cherubin, MR Karlen, DL Cerri, CEP Franco, ALC Tormena, CA Davies, CA Cerri, CC AF Cherubin, Mauricio R. Karlen, Douglas L. Cerri, Carlos E. P. Franco, Andre L. C. Tormena, Cassio A. Davies, Christian A. Cerri, Carlos C. TI Soil Quality Indexing Strategies for Evaluating Sugarcane Expansion in Brazil SO PLOS ONE LA English DT Article ID MANAGEMENT ASSESSMENT FRAMEWORK; LAND-USE CHANGE; PHYSICAL-PROPERTIES; PRODUCTION SYSTEMS; CARBON; INDICATORS; TILLAGE; PASTURES; SEQUESTRATION; CULTIVATION AB Increasing demand for biofuel has intensified land-use change (LUC) for sugarcane (Saccharum officinarum) expansion in Brazil. Assessments of soil quality (SQ) response to this LUC are essential for quantifying and monitoring sustainability of sugarcane production over time. Since there is not a universal methodology for assessing SQ, we conducted a field-study at three sites within the largest sugarcane-producing region of Brazil to develop a SQ index (SQI). The most common LUC scenario (i.e., native vegetation to pasture to sugarcane) was evaluated using six SQI strategies with varying complexities. Thirty eight soil indicators were included in the total dataset. Two minimum datasets were selected: one using principal component analysis (7 indicators) and the other based on expert opinion (5 indicators). Non-linear scoring curves were used to interpret the indicator values. Weighted and non-weighted additive methods were used to combine individual indicator scores into an overall SQI. Long-term conversion from native vegetation to extensive pasture significantly decreased overall SQ. In contrast, conversion from pasture to sugarcane had no significant impact on overall SQ at the regional scale, but site-specific responses were found. In general, sugarcane production improved chemical attributes (i.e., higher macronutrient levels and lower soil acidity); however it has negative effects on physical and biological attributes (i.e., higher soil compaction and structural degradation as well as lower soil organic carbon (SOC), abundance and diversity of macrofauna and microbial activity). Overall, we found that simple, user-friendly strategies were as effective as more complex ones for identifying SQ changes. Therefore, as a protocol for SQ assessments in Brazilian sugarcane areas, we recommend using a small number of indicators (e.g., pH, P, K, Visual Evaluation of Soil Structure -VESS scores and SOC concentration) and proportional weighting to reflect chemical, physical and biological processes within the soil. Our SQ evaluations also suggest that current approaches for expanding Brazilian sugarcane production by converting degraded pasture land to cropland can be a sustainable strategy for meeting increasing biofuel demand. However, management practices that alleviate negative impacts on soil physical and biological indicators must be prioritized within sugarcane producing areas to prevent unintentional SQ degradation over time. C1 [Cherubin, Mauricio R.; Cerri, Carlos E. P.] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, Piracicaba, SP, Brazil. [Karlen, Douglas L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Franco, Andre L. C.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. [Tormena, Cassio A.] Univ Estadual Maringa, Dept Agron, Maringa, Parana, Brazil. [Davies, Christian A.] Shell Technol Ctr Houston, Houston, TX USA. [Cerri, Carlos C.] Univ Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, SP, Brazil. RP Cherubin, MR (reprint author), Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Soil Sci, Piracicaba, SP, Brazil. EM cherubin@usp.br RI Cerri, Carlos Eduardo/C-5039-2012; OI Franco, Andre/0000-0003-3294-6848 FU Sao Paulo Research Foundation (FAPESP) [2013/17581-6, 2014/16612-8, 2012/22510-8]; National Council for Scientific and Technological Development (CNPq) - Brazil; CNPq [203376/2014-5]; Shell Technology Center Houston; Raizen FX MRC and ALCF thank the Sao Paulo Research Foundation (FAPESP) for research grants (Processes # 2013/17581-6; 2014/16612-8 and 2012/22510-8). CEPC, CCC and CAT thank the National Council for Scientific and Technological Development (CNPq) - Brazil for their productivity grants. CAT thanks the CNPq for his postdoc grant (Process 203376/2014-5). CAD thanks the Shell Technology Center Houston for providing him support in form of salaries. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of the authors are articulated in the 'author contributions' section.; The authors are grateful to Raizen for all the support received and access provided for this research. NR 74 TC 0 Z9 0 U1 4 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 3 PY 2016 VL 11 IS 3 AR e0150860 DI 10.1371/journal.pone.0150860 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG0EB UT WOS:000371735200151 PM 26938642 ER PT J AU Wen, GY Hu, X Zhao, K Wang, HL Zhang, ZY Zhang, TF Yang, JL Luo, QP Zhang, RR Pan, ZS Shao, HB Yu, QZ AF Wen, Guoyuan Hu, Xiao Zhao, Kang Wang, Hongling Zhang, Zhenyu Zhang, Tengfei Yang, Jinlong Luo, Qingping Zhang, Rongrong Pan, Zishu Shao, Huabin Yu, Qingzhong TI Molecular basis for the thermostability of Newcastle disease virus SO SCIENTIFIC REPORTS LA English DT Article ID HEPATITIS-B-VACCINE; COLD CHAIN; STRAIN I-2; PROTEOLYTIC CLEAVAGE; VILLAGE CHICKENS; PARAMYXOVIRUS GLYCOPROTEINS; HEAT-RESISTANT; FUSION PROTEIN; SENDAI VIRUS; HN PROTEIN AB Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain and thermolabile LaSota strain using reverse genetics technology. Evaluations of these chimeric NDVs demonstrated that the thermostability of NDV was dependent on the origin of HN protein. Chimeras bearing the HN protein derived from thermostable virus exhibited a thermostable phenotype, and vice versa. Both hemagglutinin and neuraminidase activities of viruses bearing the TS09-C HN protein were more thermostable than those containing LaSota HN protein. Furthermore, the newly developed thermostable virus rLS-T-HN, encoding the TS09-C HN protein in LaSota backbone, induced significantly higher antibody response than the TS09-C virus, and conferred complete protection against virulent NDV challenge. Taken together, the data suggest that the HN protein of NDV is a crucial determinant of thermostability, and the HN gene from a thermostable NDV could be engineered into a thermolabile NDV vaccine strain for developing novel thermostable NDV vaccine. C1 [Wen, Guoyuan; Hu, Xiao; Zhao, Kang; Wang, Hongling; Zhang, Tengfei; Luo, Qingping; Zhang, Rongrong; Shao, Huabin] Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430070, Peoples R China. [Wen, Guoyuan; Zhang, Zhenyu; Yang, Jinlong; Yu, Qingzhong] ARS, US Natl Poultry Res Ctr, USDA, Southeast Poultry Res Lab, Athens, GA 30605 USA. [Hu, Xiao; Zhao, Kang; Pan, Zishu] Wuhan Univ, Coll Life Sci, State Key Lab Virol, Wuhan 430072, Peoples R China. [Wen, Guoyuan] Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430070, Peoples R China. RP Yu, QZ (reprint author), ARS, US Natl Poultry Res Ctr, USDA, Southeast Poultry Res Lab, Athens, GA 30605 USA. EM qingzhong.yu@ars.usda.gov FU National Natural Science Foundation of China [31000082]; China Agriculture Research System [CARS-42-G11]; Special Fund for Agro-scientific Research in the Public Interest [201303033]; United States Department of Agriculture (USDA, ARS CRIS project) [6040-32000-067-00D] FX We thank Xiuqin Xia for excellent technical assistance and Patti Miller for critical reading of the manuscript. This work was supported by the National Natural Science Foundation of China (31000082), China Agriculture Research System (CARS-42-G11), Special Fund for Agro-scientific Research in the Public Interest (201303033), and United States Department of Agriculture (USDA, ARS CRIS project 6040-32000-067-00D). USDA is an equal opportunity provider and employer. NR 47 TC 1 Z9 1 U1 2 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAR 3 PY 2016 VL 6 AR 22492 DI 10.1038/srep22492 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DF3FY UT WOS:000371230600001 PM 26935738 ER PT J AU Bengston, DN AF Bengston, David N. TI The Futures Wheel: A Method for Exploring the Implications of Social-Ecological Change SO SOCIETY & NATURAL RESOURCES LA English DT Article DE Futures Wheel; Implications Wheel; participatory; smart group; social-ecological change; structured brainstorming; unforeseen consequences AB Change in social-ecological systems often produces a cascade of unanticipated consequences. Natural resource professionals and other stakeholders need to understand the possible implications of cascading change to prepare for it. The Futures Wheel is a smart group method that uses a structured brainstorming process to uncover and evaluate multiple levels of consequences resulting from all types of change. The output is a map of possible direct and indirect, positive and negative impacts that can be analyzed to develop strategies to promote desirable consequences and avoid undesirable ones. The Futures Wheel can help natural resource planners and decision makers anticipate unforeseen consequences of social-ecological change and become more proactive. C1 [Bengston, David N.] USDA Forest Serv, Strateg Foresight Grp, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. RP Bengston, DN (reprint author), USDA Forest Serv, Strateg Foresight Grp, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM dbengston@fs.fed.us NR 14 TC 0 Z9 0 U1 2 U2 9 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0894-1920 EI 1521-0723 J9 SOC NATUR RESOUR JI Soc. Nat. Resour. PD MAR 3 PY 2016 VL 29 IS 3 BP 374 EP 379 DI 10.1080/08941920.2015.1054980 PG 6 WC Environmental Studies; Planning & Development; Sociology SC Environmental Sciences & Ecology; Public Administration; Sociology GA DB5KI UT WOS:000368552600008 ER PT J AU Bond-Lamberty, B Bolton, H Fansler, S Heredia-Langner, A Liu, CX McCue, LA Smith, J Bailey, V AF Bond-Lamberty, Ben Bolton, Harvey Fansler, Sarah Heredia-Langner, Alejandro Liu, Chongxuan McCue, Lee Ann Smith, Jeffrey Bailey, Vanessa TI Soil Respiration and Bacterial Structure and Function after 17 Years of a Reciprocal Soil Transplant Experiment SO PLOS ONE LA English DT Article ID ORGANIC-MATTER DECOMPOSITION; SHRUB-STEPPE ECOSYSTEM; CLIMATE-CHANGE; MICROBIAL COMMUNITIES; TEMPERATURE SENSITIVITY; TERRESTRIAL ECOSYSTEMS; SEMIARID ECOSYSTEMS; NORTH-AMERICA; FOREST SOIL; GAS FLUXES AB The effects of climate change on soil organic matter-its structure, microbial community, carbon storage, and respiration response-remain uncertain and widely debated. In addition, the effects of climate changes on ecosystem structure and function are often modulated or delayed, meaning that short-term experiments are not sufficient to characterize ecosystem responses. This study capitalized on a long-term reciprocal soil transplant experiment to examine the response of dryland soils to climate change. The two transplant sites were separated by 500 m of elevation on the same mountain slope in eastern Washington state, USA, and had similar plant species and soil types. We resampled the original 1994 soil transplants and controls, measuring CO2 production, temperature response, enzyme activity, and bacterial community structure after 17 years. Over a laboratory incubation of 100 days, reciprocally transplanted soils respired roughly equal cumulative amounts of carbon as non-transplanted controls from the same site. Soils transplanted from the hot, dry, lower site to the cooler and wetter (difference of -5 degrees C monthly maximum air temperature, +50 mm yr(-1) precipitation) upper site exhibited almost no respiratory response to temperature (Q(10) of 1.1), but soils originally from the upper, cooler site had generally higher respiration rates. The bacterial community structure of transplants did not differ significantly from that of untransplanted controls, however. Slight differences in local climate between the upper and lower Rattlesnake locations, simulated with environmental control chambers during the incubation, thus prompted significant differences in microbial activity, with no observed change to bacterial structure. These results support the idea that environmental shifts can influence soil C through metabolic changes, and suggest that microbial populations responsible for soil heterotrophic respiration may be constrained in surprising ways, even as shorter-and longer-term soil microbial dynamics may be significantly different under changing climate. C1 [Bond-Lamberty, Ben] Univ Maryland, Pacific NW Natl Lab, Joint Global Change Res Inst, 5825 Univ Res Court 3500, College Pk, MD 20740 USA. [Bolton, Harvey; Fansler, Sarah; Heredia-Langner, Alejandro; Liu, Chongxuan; McCue, Lee Ann; Bailey, Vanessa] Pacific NW Natl Lab, 902 Battelle Blvd, Richland, WA 99352 USA. [Smith, Jeffrey] Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. RP Bond-Lamberty, B (reprint author), Univ Maryland, Pacific NW Natl Lab, Joint Global Change Res Inst, 5825 Univ Res Court 3500, College Pk, MD 20740 USA. EM bondlamberty@pnnl.gov RI Bond-Lamberty, Ben/C-6058-2008; Liu, Chongxuan/C-5580-2009; OI Bond-Lamberty, Ben/0000-0001-9525-4633; Bailey, Vanessa/0000-0002-2248-8890; McCue, Lee Ann/0000-0003-4456-517X FU U.S. Department of Energy, Office of Science, Biological and Environmental Research (BER) as part of the Terrestrial Ecosystem Sciences Program; Signature Discovery Initiative at the Pacific Northwest National Laboratory (PNNL); BER; DOE [DE-AC05-76RL01830] FX This research was supported by the U.S. Department of Energy, Office of Science, Biological and Environmental Research (BER) as part of the Terrestrial Ecosystem Sciences Program and the Signature Discovery Initiative at the Pacific Northwest National Laboratory (PNNL). Carbon analyses were performed at the Environmental Molecular Sciences Laboratory, a DOE Office of Science user facility sponsored by the BER and located at PNNL. PNNL is operated for DOE by Battelle Memorial Institute under contract DE-AC05-76RL01830. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; This research was supported by the U.S. Department of Energy, Office of Science, Biological and Environmental Research (BER) as part of the Terrestrial Ecosystem Sciences Program and the Signature Discovery Initiative at the Pacific Northwest National Laboratory (PNNL). Carbon analyses were performed at the Environmental Molecular Sciences Laboratory, a DOE Office of Science user facility sponsored by the BER and located at PNNL. PNNL is operated for DOE by Battelle Memorial Institute under contract DE-AC05-76RL01830. This paper is dedicated to the memory of Jeff Smith. NR 64 TC 2 Z9 2 U1 6 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAR 2 PY 2016 VL 11 IS 3 AR e0150599 DI 10.1371/journal.pone.0150599 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DG0AN UT WOS:000371724200105 PM 26934712 ER PT J AU Hale, AL Viator, RP Eggleston, G Hodnett, G Stelly, DM Boykin, D Miller, DK AF Hale, Anna L. Viator, Ryan P. Eggleston, Gillian Hodnett, George Stelly, David M. Boykin, Debbie Miller, Donnie K. TI Estimating Broad Sense Heritability and Investigating the Mechanism of Genetic Transmission of Cold Tolerance Using Mannitol as a Measure of Post-freeze Juice Degradation in Sugarcane and Energycane (Saccharum spp.) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE energycane; freeze deterioration; Leuconostoc bacteria; mannitol; sugarcane ID PROCESSING PROBLEMS; GENOME STRUCTURE; DETERIORATION; VARIETIES; FACTORIES AB In approximately 25% of the sugarcane-producing countries worldwide, conventional sugarcane (Saccharum spp. hybrids) is exposed to damaging freezes. A study was conducted during the 2009 and 2010 harvest seasons to compare late season freeze tolerance among three groups: commercial Louisiana sugarcane genotypes, early generation genotypes selected for cold tolerance in the U.S. Department of Agriculture sugarcane breeding, programs at Houma, LA, and Canal Point, FL, and potential energycane genotypes selected for high total biomass per acre. Mannitol concentrations in cane juice following freezing temperatures were determined to evaluate levels of cold tolerance. Genotypes selected for cold tolerance in Houma, LA, had significantly more late-season freeze tolerance than commercial sugarcane genotypes and genotypes selected in Canal Point, FL. Genotypes showing the most cold tolerance were Ho02-146 and Ho02-152, and those that were most highly susceptible were US87-1006 and US87-1003 (early-generation breeding genotypes) and L99-233 (commercial genotype). Broad-sense heritability for late-season cold tolerance in the two-year study was estimated at g(2) = 0.78. The enzymatic mannitol analysis successfully differentiated high-fiber energycane genotypes from those from other sources. C1 [Hale, Anna L.; Viator, Ryan P.] ARS, Sugarcane Res Unit, USDA, 5883 USDA Rd, Houma, LA 70360 USA. [Eggleston, Gillian] ARS, Commod Utilizat Res Unit, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Hodnett, George; Stelly, David M.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Boykin, Debbie] ARS, Southeast Area, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. [Miller, Donnie K.] Louisiana State Univ, AgCtr Northeast Res Stn, 4589 Highway 605, St Joseph, LA 71366 USA. RP Hale, AL (reprint author), ARS, Sugarcane Res Unit, USDA, 5883 USDA Rd, Houma, LA 70360 USA.; Hale, AL (reprint author), PhD & Associates LLC, 122 Newsom Dr, Houma, LA 70360 USA. EM Anna.Hale@ars.usda.gov NR 25 TC 1 Z9 1 U1 1 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 2 PY 2016 VL 64 IS 8 BP 1657 EP 1663 DI 10.1021/acs.jafc.5b03803 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DF6GN UT WOS:000371454000002 PM 26885566 ER PT J AU Adedipe, OE Johanningsmeier, SD Truong, VD Yencho, GC AF Adedipe, Oluwatosin E. Johanningsmeier, Suzanne D. Truong, Van-Den Yencho, G. Craig TI Development and Validation of a Near-Infrared Spectroscopy Method for the Prediction of Acrylamide Content in French-Fried Potato SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE acrylamide; near-infrared spectroscopy (NIRS); partial least-squares (PLS) regression; potato; Solanum tuberosum ID EFFECTIVE QUALITY-CONTROL; DRY-MATTER; MITIGATION STRATEGY; INCOMING POTATOES; COLOR; CHIPS; ONCOGENICITY; RATS AB This study investigated the ability of near-infrared spectroscopy (NIRS) to predict acrylamide content in French fried potato. Potato flour spiked with acrylamide (50-8000 mu g/kg) was used to determine if acrylamide could be accurately predicted in a potato matrix. French fries produced with various pretreatments and cook times (n = 84) and obtained from quick service restaurants (n = 64) were used for model development and validation. Acrylamide was quantified using gas chromatography-mass spectrometry, and reflectance spectra (400-2500 nm) of each freeze-dried sample Were captured on a Foss XDS Rapid Content Analyzer-NIR spectrometer. Partial least-squares (PLS) discriminant analysis and PLS regression modeling demonstrated that NIRS could accurately detect acrylamide content as low as 50 mu g/kg in the model potato matrix. Prediction errors of 135 mu g/kg (R-2 = 0.98) and 255 mu g/kg (R-2 = 0.93) were achieved with the best PLS models for acrylamide prediction in Russet Norkotah French-fried potato and multiple samples of unknown varieties, respectively. The findings indicate that NIRS can be used as a screening tool in potato breeding and potato processing research to reduce acrylamide in the food supply. C1 [Adedipe, Oluwatosin E.; Yencho, G. Craig] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Johanningsmeier, Suzanne D.; Truong, Van-Den] ARS, USDA, Southeast Area, Food Sci Res Unit, Raleigh, NC 27695 USA. RP Johanningsmeier, SD (reprint author), ARS, USDA, Southeast Area, Food Sci Res Unit, Raleigh, NC 27695 USA. EM suzanne.johanningsmeier@ars.usda.gov FU USDA NIFA SCRI Grant [2011-51181-3062] FX We acknowledge funding for this research from USDA NIFA SCRI Grant 2011-51181-3062. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or North Carolina Agricultural Research Service, nor does it imply approval to the exclusion of other products that may be suitable. NR 33 TC 1 Z9 1 U1 14 U2 34 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAR 2 PY 2016 VL 64 IS 8 BP 1850 EP 1860 DI 10.1021/acs.jafc.5b04733 PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DF6GN UT WOS:000371454000023 PM 26806330 ER PT J AU Kaplan, BS Russier, M Jeevan, T Marathe, B Govorkova, EA Russell, CJ Kim-Torchetti, M Choi, YK Brown, I Saito, T Stallknecht, DE Krauss, S Webby, RJ AF Kaplan, Bryan S. Russier, Marion Jeevan, Trushar Marathe, Bindumadhav Govorkova, Elena A. Russell, Charles J. Kim-Torchetti, Mia Choi, Young Ki Brown, Ian Saito, Takehiko Stallknecht, David E. Krauss, Scott Webby, Richard J. TI Novel Highly Pathogenic Avian A(H5N2) and A(H5N8) Influenza Viruses of Clade 2.3.4.4 from North America Have Limited Capacity for Replication and Transmission in Mammals SO MSPHERE LA English DT Article DE avian influenza virus; H5N2; H5N8; mammals; viral replication ID A H5N1 VIRUSES; HEMAGGLUTININ PROTEIN; RECEPTOR SPECIFICITY; MIGRATORY BIRDS; HUMAN INFECTION; SOUTH-KOREA; FERRETS; PH; ACTIVATION; POULTRY AB Highly pathogenic influenza A(H5N8) viruses from clade 2.3.4.4 were introduced to North America by migratory birds in the fall of 2014. Reassortment of A(H5N8) viruses with avian viruses of North American lineage resulted in the generation of novel A(H5N2) viruses with novel genotypes. Through sequencing of recent avian influenza viruses, we identified PB1 and NP gene segments very similar to those in the viruses isolated from North American waterfowl prior to the introduction of A(H5N8) to North America, highlighting these bird species in the origin of reassortant A(H5N2) viruses. While they were highly virulent and transmissible in poultry, we found A(H5N2) viruses to be low pathogenic in mice and ferrets, and replication was limited in both hosts compared with those of recent highly pathogenic avian influenza (HPAI) H5N1 viruses. Molecular characterization of the hemagglutinin protein from A(H5N2) viruses showed that the receptor binding preference, cleavage, and pH of activation were highly adapted for replication in avian species and similar to those of other 2.3.4.4 viruses. In addition, North American and Eurasian clade 2.3.4.4 H5NX viruses replicated to significantly lower titers in differentiated normal human bronchial epithelial cells than did seasonal human A(H1N1) and highly pathogenic A(H5N1) viruses isolated from a human case. Thus, despite their having a high impact on poultry, our findings suggest that the recently emerging North American A(H5N2) viruses are not expected to pose a substantial threat to humans and other mammals without further reassortment and/or adaptation and that reassortment with North American viruses has not had a major impact on viral phenotype. IMPORTANCE Highly pathogenic H5 influenza viruses have been introduced into North America from Asia, causing extensive morbidity and mortality in domestic poultry. The introduced viruses have reassorted with North American avian influenza viruses, generating viral genotypes not seen on other continents. The experiments and analyses presented here were designed to assess the impact of this genetic diversification on viral phenotypes, particularly as regards mammalian hosts, by comparing the North American viruses with their Eurasian precursor viruses. C1 [Kaplan, Bryan S.; Russier, Marion; Jeevan, Trushar; Marathe, Bindumadhav; Govorkova, Elena A.; Russell, Charles J.; Krauss, Scott; Webby, Richard J.] St Jude Childrens Res Hosp, Dept Infect Dis, 332 N Lauderdale St, Memphis, TN 38105 USA. [Stallknecht, David E.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Athens, GA USA. [Kim-Torchetti, Mia] USDA, Natl Vet Serv Labs, Technol & Anal Serv Vet Serv, Anim & Plant Hlth Inspect Serv, Ames, IA 50010 USA. [Choi, Young Ki] Chungbuk Natl Univ, Coll Med, Cheongju, South Korea. [Choi, Young Ki] Chungbuk Natl Univ, Med Res Inst, Cheongju, South Korea. [Brown, Ian] Anim & Plant Hlth Agcy, Addlestone, Surrey, England. [Saito, Takehiko] Natl Agr & Food Res Org, Influenza & Prion Dis Res Ctr, Natl Inst Anim Hlth, Tsukuba, Ibaraki, Japan. EM Richard.Webby@stjude.org OI Russier, Marion/0000-0002-9633-9804 FU National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services [HHSN272201400006C]; American Lebanese Syrian Associated Charities (ALSAC) FX This study was supported by the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services (contract no. HHSN272201400006C), and the American Lebanese Syrian Associated Charities (ALSAC). NR 50 TC 3 Z9 3 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2379-5042 J9 MSPHERE JI mSphere PD MAR-APR PY 2016 VL 1 IS 2 AR e00003-16 DI 10.1128/mSphere.00003-16 PG 17 WC Microbiology SC Microbiology GA EI6DH UT WOS:000392584700001 ER PT J AU Li, LH Ma, JC Ibekwe, AM Wang, Q Yang, CH AF Li, Lihua Ma, Jincai Ibekwe, A. Mark Wang, Qi Yang, Ching-Hong TI Cucumber Rhizosphere Microbial Community Response to Biocontrol Agent Bacillus subtilis B068150 SO AGRICULTURE-BASEL LA English DT Article DE cucumber; Bacillus subtilis; Microbial community; colonization; diversity ID GRADIENT GEL-ELECTROPHORESIS; SOIL-BORNE PATHOGENS; PSEUDOMONAS-FLUORESCENS; BIOLOGICAL-CONTROL; DISEASE SUPPRESSION; FUSARIUM-WILT; SOILBORNE PATHOGENS; PLANT-DISEASE; RIBOSOMAL-RNA; DIVERSITY AB Gram-positive bacteria Bacillus subtilis B068150 has been used as a biocontrol agent against the pathogen Fusarium oxysporum cucumerinum. Cucumber was grown in three soils with strain B068150 inoculated in a greenhouse for 90 days, and the colonization ability of strain B068150 in cucumber rhizosphere and non-rhizosphere soils was determined. Changes in total bacteria and fungi community composition and structures using denaturing gradient gel electrophoresis (DGGE) and sequencing were determined. Colony counts showed that B068150 colonization in the rhizosphere was significantly higher (p < 0.001) than in non-rhizosphere soils. Based on our data, the introduction of B. bacillus B068150 did not change the diversity of microbial communities significantly in the rhizosphere of three soils. Our data showed that population density of B068150 in clay soil had a significant negative correlation on bacterial diversity in cucumber rhizosphere in comparison to loam and sandy soils, suggesting that the impact of B068150 might be soil specific. C1 [Li, Lihua; Wang, Qi] China Agr Univ, Coll Agron & Biotechnol, Dept Plant Pathol, MOA,Key Lab Plant Pathol, Beijing 100193, Peoples R China. [Li, Lihua; Ibekwe, A. Mark] ARS, USDA, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Li, Lihua] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Zhongguancunnandajie 12, Beijing 100081, Peoples R China. [Ma, Jincai] Jilin Univ, Coll Environm & Resources, Changchun 130021, Jilin, Peoples R China. [Yang, Ching-Hong] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA. RP Wang, Q (reprint author), China Agr Univ, Coll Agron & Biotechnol, Dept Plant Pathol, MOA,Key Lab Plant Pathol, Beijing 100193, Peoples R China.; Ibekwe, AM (reprint author), ARS, USDA, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM lilhok@hotmail.com; majincai@hotmail.com; Mark.Ibekwe@ars.usda.gov; wangqi@cau.edu.cn; chyang@uwm.edu OI Ma, Jincai/0000-0002-0792-0251 FU National Natural Science Foundation of China [31101480]; Program for Beijing Excellent Talent [2012D009007000001]; Specialized Research Fund for the Doctoral Program of Higher Education [20110008120013]; Research Growth Initiative of the University of Wisconsin-Milwaukee (UWM); USDA-ARS FX This work was supported by National Natural Science Foundation of China (Grant No. 31101480), The Program for Beijing Excellent Talent (Grant No. 2012D009007000001), Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20110008120013), the Research Growth Initiative of the University of Wisconsin-Milwaukee (UWM), and in part by the 214 Manure and Byproduct Utilization Project of the USDA-ARS. Mention of trademark or propriety products in this manuscript does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 68 TC 0 Z9 0 U1 0 U2 0 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2077-0472 J9 AGRICULTURE-BASEL JI Agriculture-Basel PD MAR PY 2016 VL 6 IS 1 AR 2 DI 10.3390/agriculture6010002 PG 15 WC Agronomy SC Agriculture GA EH2SC UT WOS:000391617300002 ER PT J AU Stevenson, DJ Lynch, TB Saud, P Heineman, R Holeman, R Wilson, D Anderson, K Cerny, C Guldin, JM AF Stevenson, Douglas J. Lynch, Thomas B. Saud, Pradip Heineman, Robert Holeman, Randal Wilson, Dennis Anderson, Keith Cerny, Chris Guldin, James M. TI Winter in the Ouchitas-A severe winter storm signature in Pinus echinata in the Ouachita Mountains of Oklahoma and Arkansas, USA SO DENDROCHRONOLOGIA LA English DT Article DE Ice storm; Snow Storm; Severe winter storm; Drought; Pinus echinata Mill; Ouachita Mountains ID UNITED-STATES; ICE STORMS; FORESTS; AGREEMENT AB Each year severe winter storms (approximate to ice storms) damage trees throughout the southern USA. Arkansas and Oklahoma have a history of severe winter storms. To extend that history back beyond the reach of written records, a distinctive tree ring pattern or signature is needed. Storm-caused breakage, branch loss and bending stress provide that signature. We found a severe storm signature in shortleaf pine (Pinus echinata). We used three published site chronologies, a set of five new site chronologies from a growth-and-yield study conducted by Oklahoma State University and the unpublished Shortleaf Canyon chronology from a master's thesis at the University of Arkansas. Our method is based on two ring width values for the first and second growing seasons after the storm standardized to the ring widths of the seven growing seasons after the storm. Concordance between storm years predicted by tree ring patterns and actual storm years was tested using Cohen's Kappa. Concern about confounding of ice storm signals by droughts led us to test concordance between severe storms and drought in July, August and September; results were inconclusive but stand as a warning that these two phenomena cannot be distinguished with certainty in the tree ring record. Damaging severe storms occurred in about 2.8% of all years. Two out of three storms identified as "severe" produced glaze icing. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Stevenson, Douglas J.; Lynch, Thomas B.; Saud, Pradip] Oklahoma State Univ, Dept Nat Resources & Environm Management, Stillwater, OK 74078 USA. [Heineman, Robert; Holeman, Randal; Wilson, Dennis; Anderson, Keith] Oklahoma State Univ, Kiamichi Forestry Res Stn, Idabel, OK 74745 USA. [Cerny, Chris] Univ Arkansas, Tree Ring Lab, Dept Geosci, Fayetteville, AR 72701 USA. [Guldin, James M.] US Forest Serv, Southern Res Stn, USDA, Hot Springs, AR 72701 USA. RP Stevenson, DJ (reprint author), Oklahoma State Univ, Dept Nat Resources & Environm Management, Stillwater, OK 74078 USA. EM djohns1066@yahoo.com; tom.lynch@okstate.edu; pradip.saud@okstate.edu; bob.heineman@okstate.edu; randy.holeman@okslate.eclu; dennis.wilson@okstate.edu; keith.anderson@okstate.edu; jguldin@fs.fed.us OI Saud, Pradip/0000-0002-2045-2827 FU [OKL0-2843] FX The authors thank personnel of the Ouachita National Forest for help finding study sites and preserving them. Thanks are due to David W. Stahle of the University of Arkansas' Tree Ring Laboratory for technical advice and training and to Stephen Hallgren for the use of his microscope and measuring Table Special thanks are due the late Paul A. Murphy, former Principal Mensurationist for the Southern Research Station who cooperated with OSU in the establishment of a growth and yield study of shortleaf pine on the Ouachita National Forest and established the Sand Lick study site. We also thank Mark Gregory of OSU for his help in mapping. This project was funded under OKL0-2843 which was not directly involved with study design or data collection, analysis or interpretation. NR 43 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1125-7865 EI 1612-0051 J9 DENDROCHRONOLOGIA JI Dendrochronologia PD MAR PY 2016 VL 37 BP 107 EP 115 DI 10.1016/j.dendro.2016.01.001 PG 9 WC Plant Sciences; Forestry; Geography, Physical SC Plant Sciences; Forestry; Physical Geography GA EA8EO UT WOS:000386868300012 ER PT J AU Hughes, SO Frazier-Wood, AC AF Hughes, Sheryl O. Frazier-Wood, Alexis C. TI Satiety and the Self-Regulation of Food Take in Children: a Potential Role for Gene-Environment Interplay SO CURRENT OBESITY REPORTS LA English DT Article DE Children; Eating; Satiety; Self-regulation; Gene-environment interplay ID EATING BEHAVIOR QUESTIONNAIRE; BODY-MASS INDEX; MATERNAL FEEDING PRACTICES; VIVA LA FAMILIA; CHILDHOOD OBESITY; CALORIC COMPENSATION; COGNITIVE PERFORMANCE; EXECUTIVE FUNCTIONS; HISPANIC CHILDREN; YOUNG-CHILDREN AB Child eating self-regulation refers to behaviors that enable children to start and stop eating in a manner consistent with maintaining energy balance. Perturbations in these behaviors, manifesting as poorer child eating self-regulation, are associated with higher child weight status. Initial research into child eating self-regulation focused on the role of parent feeding styles and behaviors. However, we argue that child eating self-regulation is better understood as arising from a complex interplay between the child and their feeding environment, and highlight newer research into the heritable child characteristics, such as cognitive ability, that play an important role in this dynamic. Therefore, child eating self-regulation arises from gene-environment interactions. Identifying the genes and environmental influences contributing to these will help us tailor our parental feeding advice to the unique nature of the child. In this way, we will devise more effective advice for preventing childhood obesity. C1 [Hughes, Sheryl O.; Frazier-Wood, Alexis C.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. RP Frazier-Wood, AC (reprint author), Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM shughes@bcm.edu; lekki.wood@gmail.com FU NICHD NIH HHS [R01 HD062567] NR 65 TC 0 Z9 0 U1 5 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 2162-4968 J9 CURR OBES REP JI Curr. Obes. Rep. PD MAR PY 2016 VL 5 IS 1 BP 81 EP 87 DI 10.1007/s13679-016-0194-y PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA EB0SH UT WOS:000387055300010 PM 26847550 ER PT J AU Campbell, JB Nath, R Gadau, J Fox, T DeGrandi-Hoffman, G Harrison, JF AF Campbell, Jacob B. Nath, Rachna Gadau, Juergen Fox, Trevor DeGrandi-Hoffman, Gloria Harrison, Jon F. TI The fungicide Pristine (R) inhibits mitochondrial function in vitro but not flight metabolic rates in honey bees SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Mitochondria; Pristine (R); Honey bee; Flight; Fungicide ID APIS-MELLIFERA; MUSCLE DEVELOPMENT; WORKER HONEYBEES; PERFORMANCE; PESTICIDES; POLLEN; AGE; L. AB Honey bees and other pollinators are exposed to fungicides that act by inhibiting fungal mitochondria. Here we test whether a common fungicide (Pristine(R)) inhibits the function of mitochondria of honeybees, and whether consumption of ecologically-realistic concentrations can cause negative effects on the mitochondria of flight muscles, or the capability for flight, as judged by CO2 emission rates and thorax temperatures during flight. Direct exposure of mitochondria to Pristine(R) levels above 5 ppm strongly inhibited mitochondrial oxidation rates in vitro. However, bees that consumed pollen containing Pristine(R) at ecologically-realistic concentrations (approximate to 1 ppm) had normal flight CO2 emission rates and thorax temperatures. Mitochondria isolated from the flight muscles of the Pristine(R)-consuming bees had higher state 3 oxygen consumption rates than control bees, suggesting that possibly Pristine(R)-consumption caused compensatory changes in mitochondria. It is likely that the lack of a strong functional effect of Pristine(R)-consumption on flight performance and the in vitro function of flight muscle mitochondria results from maintenance of Pristine(R) levels in the flight muscles at much lower levels than occur in the food, probably due to metabolism and detoxification. As Pristine(R) has been shown to negatively affect feeding rates and protein digestion of honey bees, it is plausible that Pristine(R) consumption negatively affects gut wall function (where mitochondria may be exposed to higher concentrations of Pristine(R)). (C) 2015 Elsevier Ltd. All rights reserved. C1 [Campbell, Jacob B.; Nath, Rachna; Gadau, Juergen; Fox, Trevor; Harrison, Jon F.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [DeGrandi-Hoffman, Gloria] USDA, ARS, Honey Bee Res, 2000 East Allen Rd, Tucson, AZ 85719 USA. RP Campbell, JB (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. EM Jacob.Campbell.1@asu.edu; rnath@asu.edu; juergen.Gadau@asu.edu; tpfox1@acu.edu; Gloria.Hoffman@ars.usda.gov; j.harrison@asu.edu OI Nath, Rachna/0000-0002-7043-5478 FU NSF IOS [1122157]; USDA-ARS CRIS Project [2022-21000-018-00D] FX This research was partially supported by NSF IOS 1122157, and USDA-ARS CRIS Project 2022-21000-018-00D. NR 39 TC 1 Z9 1 U1 8 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAR PY 2016 VL 86 BP 11 EP 16 DI 10.1016/j.jinsphys.2015.12.003 PG 6 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA DY0IC UT WOS:000384779500002 PM 26685059 ER PT J AU Walse, SS Jimenez, LR Hall, WA Tebbets, JS Obenland, DM AF Walse, Spencer S. Jimenez, Leonel R. Hall, Wiley A. Tebbets, J. Steven Obenland, David M. TI Optimizing postharvest methyl bromide treatments to control spotted wing drosophila, Drosophila suzukii, in sweet cherries from Western USA SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Spotted wing drosophila; Postharvest fumigation; Methyl bromide; Sweet cherries; Quarantine treatments ID FRUITS AB Methyl bromide (MB) chamber fumigations were evaluated for postharvest control of spotted wing drosophila (SWD), Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), in fresh sweet cherry exports from Western USA. Sweet cherries were infested with SWD, incubated to maximize numbers of the most MB-tolerant specimens (ca. 60 to 108-h old at fumigation, 88% 3rd & 2nd instars), buried amongst uninfested fruit in bins consistent with commercial practice, cooled to an average pulp temperature >= 8.3 degrees C, and then fumigated in a chamber. Treatment efficacy was diagnosed by the percentage of survivors emerging as adults from fumigated cherries relative to that from non-fumigated control cherries. A kinetic model of sorption was developed based on the measurement of MB and how calculated exposures varied across the fumigation trials. The model describes how to manipulate the applied MB dose, fumigation duration, and the load factor so that the resultant exposure is adequate for SWD control across various pulp temperatures when cherries are fumigated in wooden versus plastic bins. Results are discussed in the context of graduation toward optimized quarantine fumigation schedules for control of SWD, which will promote more strategic technical and economic Quarantine Pre-shipment (QPS) use of MB. Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. C1 [Walse, Spencer S.; Hall, Wiley A.; Tebbets, J. Steven; Obenland, David M.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. [Jimenez, Leonel R.] Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA 92697 USA. RP Walse, SS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM spencer.walse@ars.usda.gov; leonelj@uci.edu; steve.tebbets@ars.usda.gov; david.obenland@ars.usda.gov FU United States Department of Agriculture; California Cherry Research Board; Washington Tree Fruit Research Commission; Oregon Sweet Cherry Commission; Northwest Fruit Exporters FX This research was financed by the United States Department of Agriculture, California Cherry Research Board, Washington Tree Fruit Research Commission, Oregon Sweet Cherry Commission, and Northwest Fruit Exporters. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 0 Z9 0 U1 3 U2 8 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD MAR PY 2016 VL 19 IS 1 BP 223 EP 232 DI 10.1016/j.aspen.2015.12.012 PG 10 WC Entomology SC Entomology GA DX6TD UT WOS:000384515100034 ER PT J AU Molina, CI Puliafico, KP AF Molina, Carlos I. Puliafico, Kenneth P. TI Life cycles of dominant mayflies (Ephemeroptera) on a torrent of the high Bolivian Andes SO REVISTA DE BIOLOGIA TROPICAL LA English DT Article DE Ephemeroptera; Meridialaris; Andesiops; life cycle; high Andes; Bolivia ID SOUTHERN ONTARIO; MOUNTAIN STREAM; TROPICAL STREAM; COSTA-RICA; HISTORY; TRICHOPTERA; BAETIDAE; INSECTS; BIOLOGY AB The mayflies of the temperate and cold zones have well-synchronized life cycles, distinct cohorts, short emergence and flight periods. In contrast, aquatic insects from the tropical zones are characterized by multivoltine life cycles, "non-discernible cohorts" and extended flight periods throughout the year. This report is the first observation of life cycle patterns made of two species of mayflies on a torrent in the high elevation Bolivian Andes. The samples were taken from four sites and four periods during a hydrological season. The life cycle of each species was examined using size-class frequency analysis and a monthly modal progression model (von Bertalanffy's model) to infer the life cycle synchrony type. These first observations showed a moderately synchronized univoltine life cycle for Andesiops peruvianzts (Ulmer, 1920), whereas Meridialaris tintinnabula Pescador and Peters (1987), had an unsynchronized multivoltine life cycle. These results showed that the generalization of all aquatic insects as unsynchronized multivoltine species in the Andean region may not be entirely accurate since there is still a need to further clarify the life cycle patterns of the wide variety of aquatic insects living in this high elevation tropical environment. C1 [Molina, Carlos I.] Univ Mayor San Andres, Inst Ecol, Casilla 10077, La Paz, Bolivia. [Puliafico, Kenneth P.] US Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, POB 236, Volcano, HI 96785 USA. [Molina, Carlos I.; Puliafico, Kenneth P.] Univ Copenhagen, Nat Hist Museum Denmark, Univ Pk 15, DK-2100 Copenhagen O, Denmark. RP Molina, CI (reprint author), Univ Mayor San Andres, Inst Ecol, Casilla 10077, La Paz, Bolivia.; Molina, CI (reprint author), Univ Copenhagen, Nat Hist Museum Denmark, Univ Pk 15, DK-2100 Copenhagen O, Denmark. EM camoar6088@gmail.com; puliafico@gmail.com NR 29 TC 0 Z9 0 U1 1 U2 1 PU REVISTA DE BIOLOGIA TROPICAL PI SAN JOSE PA UNIVERSIDAD DE COSTA RICA CIUDAD UNIVERSITARIA, SAN JOSE, 00000, COSTA RICA SN 0034-7744 EI 2215-2075 J9 REV BIOL TROP JI Rev. Biol. Trop. PD MAR PY 2016 VL 64 IS 1 BP 275 EP 287 PG 13 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DU1FK UT WOS:000381953400023 ER PT J AU Schantz, MC Sheley, RL James, JJ Hamerlynck, EP AF Schantz, Merilynn C. Sheley, Roger L. James, Jeremy J. Hamerlynck, Erik P. TI ROLE OF DISPERSAL TIMING AND FREQUENCY IN ANNUAL GRASS-INVADED GREAT BASIN ECOSYSTEMS: HOW MODIFYING SEEDING STRATEGIES INCREASES RESTORATION SUCCESS SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID PERENNIAL GRASSES; PLANT COMMUNITY; BROMUS-TECTORUM; RECRUITMENT; LIMITATION; EMERGENCE; DENSITY; STEPPE; GROWTH; PHOTOSYNTHESIS AB Seed dispersal dynamics strongly affect plant community assembly in restored annual grass-infested ecosystems. Modifying perennial grass seeding rates and frequency may increase perennial grass establishment, yet these impacts have not yet been quantified. To assess these effects, we established a field experiment consisting of 288 plots (1 m(2)) in an eastern Oregon annual grass-dominated shrubsteppe ecosystem. In this study, the amount, timing, and frequency of perennial grass seeding events, soil moisture availability, and annual grass seed bank density were manipulated. We found that more frequent perennial grass seeding events combined with high perennial grass seeding rates produced the highest perennial grass density and biomass 2 years following seeding. However, we also found that if annual grass seed density was 1500 seeds . m(-2) or higher, perennial grass density and biomass decreased, regardless of seeding strategy. Because of this finding, it appears that a threshold is crossed between 150 and 1500 annual grass seeds . m(-2). Adding water in the first growing season initially facilitated perennial grass establishment but only produced higher perennial grass density following the second growing season when annual grass density was lowest. Assessing the existing annual grass seed bank prior to seeding can likely forecast restoration outcomes because high annual grass seed densities likely interfere with and reduce perennial grass recruitment. In addition, if annual grass seeding density is 1500 seeds . m(-2) or lower, modifying the temporal patterns of perennial grass seed arrival will increase the likelihood that a perennial grass seed finds a safe-site. C1 [Schantz, Merilynn C.] Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97332 USA. [Sheley, Roger L.; Hamerlynck, Erik P.] ARS, USDA, Burns, OR 97720 USA. [James, Jeremy J.] Univ Calif, Div Agr & Nat Resources, Sierra Foothills Res & Extens Ctr, Browns Valley, CA 95918 USA. RP Schantz, MC (reprint author), Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97332 USA. EM merilynn.schantz@ars.usda.gov FU USDA-ARS EBIPM research project FX We thank Brett Bingham and Anna Masterson for field and laboratory assistance. We also thank Dr. Kirk Reinhart, Dr. Michael Borman, Dr. Paul Doescher, Mrs. Diona Austill, and any anonymous reviewers for their reviews and comments. This work was funded by the USDA-ARS EBIPM research project. NR 47 TC 0 Z9 0 U1 10 U2 10 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD MAR PY 2016 VL 76 IS 1 BP 36 EP 52 PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DS6GB UT WOS:000380879000005 ER PT J AU Ball, VE Nehring, R Wang, SL AF Ball, V. Eldon Nehring, Richard Wang, Sun Ling TI Productivity Growth in U.S. Agriculture: 1948-2013 SO INTERNATIONAL PRODUCTIVITY MONITOR LA English DT Article ID UNITED-STATES AB It is widely reported that productivity growth is the main contributor to output growth in U.S. agriculture. This article provides estimates of output growth over the postwar period and decomposes that growth into the contributions of input growth and productivity growth. The analysis is based on recently revised production accounts for agriculture. Our findings are fully consistent with those reported in the literature. Productivity growth dominates input growth as a source of output growth in the sector. C1 [Ball, V. Eldon; Nehring, Richard; Wang, Sun Ling] USDA, Econ Res Serv, Washington, DC 20024 USA. RP Ball, VE (reprint author), USDA, Econ Res Serv, Washington, DC 20024 USA. EM eball@ers.usda.gov; rnehring@ers.usda.gov; slwang@ers.usda.gov NR 28 TC 0 Z9 0 U1 0 U2 0 PU CENTRE STUDY LIVING STANDARDS PI OTTAWA PA 111 SPARKS ST, STE 500, OTTAWA, ON K1P 5B5, CANADA SN 1492-9759 EI 1492-9767 J9 INT PRODUCT MONIT JI Int. Product. Monit. PD SPR PY 2016 VL 30 BP 64 EP 76 PG 13 WC Economics SC Business & Economics GA DQ4DW UT WOS:000379155100004 ER PT J AU Bragg, DC AF Bragg, Don C. TI Forestry in the US South: A History SO ARKANSAS HISTORICAL QUARTERLY LA English DT Book Review C1 [Bragg, Don C.] USDA, Forest Serv, Southern Res Stn, Monticello, FL USA. RP Bragg, DC (reprint author), USDA, Forest Serv, Southern Res Stn, Monticello, FL USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU ARKANSAS HISTORICAL ASSOC PI FAYETTEVILLE PA UNIV ARKANSAS, OLD MAIN 416 DEPT OF HISTORY, FAYETTEVILLE, AR 72701 USA SN 0004-1823 EI 2327-1213 J9 ARKANSAS HIST QUART JI Ark. Hist. Q. PD SPR PY 2016 VL 75 IS 1 BP 73 EP 75 PG 3 WC History SC History GA DP2FJ UT WOS:000378303200009 ER PT J AU Johnson, PJ Hesler, LA AF Johnson, Paul J. Hesler, Lauren A. TI TENOMERGA CINEREA (SAY) (COLEOPTERA: CUPEDIDAE), A NEW SOUTH DAKOTA STATE RECORD FOR THE FAMILY SO COLEOPTERISTS BULLETIN LA English DT Editorial Material C1 [Johnson, Paul J.] S Dakota State Univ, Insect Biodivers Lab, Brookings, SD 57007 USA. [Hesler, Lauren A.] S Dakota State Univ, USDA APHIS PPQ, Brookings, SD 57007 USA. RP Johnson, PJ (reprint author), S Dakota State Univ, Insect Biodivers Lab, Brookings, SD 57007 USA. NR 5 TC 0 Z9 0 U1 2 U2 2 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD MAR PY 2016 VL 70 IS 1 BP 194 EP 195 PG 2 WC Entomology SC Entomology GA DO8YN UT WOS:000378071600029 ER PT J AU Hagerty, CH Cuesta-Marcos, A Cregan, P Song, QJ McClean, P Myers, JR AF Hagerty, Christina H. Cuesta-Marcos, Alfonso Cregan, Perry Song, Qijian McClean, Phil Myers, James R. TI Mapping Snap Bean Pod and Color Traits, in a Dry Bean x Snap Bean Recombinant Inbred Population SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE single nucleotide polymorphism; SNP; quantitative trait locus; QTL; linkage mapping; common bean ID PHASEOLUS-VULGARIS L.; TRANSGRESSIVE SEGREGATION; QTL ANALYSIS; LINKAGE MAP; COMMON; IDENTIFICATION; RESISTANCE; ARCHITECTURE; CROSS; LOCI AB Snap bean (Phaseolus vulgaris L.) breeding programs are tasked with developing cultivars that meet the standards of the vegetable processing industry and ultimately that of the consumer, all the while matching or exceeding the field performance of existing cultivars. While traditional breeding methods have had a long history of meeting these requirements, genetic marker technology, combined with the knowledge of important quantitative trait loci (QTL), can accelerate breeding efforts. In contrast to dry bean, snap bean immature pods and seeds are consumed as a vegetable. Several pod traits are important in snap bean including: reduced pod wall fiber, absence of pod suture strings, and thickened, succulent pod walls. In addition, snap bean pods are selected for round pod cross section, and pods tend to be longer with cylindrical seed shape. Seed color is an important trait in snap bean, especially those used for processing, as processors prefer white-seeded cultivars. The objective of this study was to investigate the genetic control of traits important to snap bean producers and processors. RR6950, a small seeded brown indeterminate type IIIA dry bean accession, was crossed to the Oregon State University (OSU) breeding line OSU5446, a type I Blue Lake four-sieve breeding line to produce the RR138 F-4:6 recombinant inbred (RI) mapping population. We evaluated the RR138 RI population for processing and morphological traits, especially those affecting pods. The RR138 population was genotyped with the BARCBean6K_3 Beadchip, and single nucleotide polymorphisms (SNPs) were used to assemble a linkage map, and identify QTL for pod traits. The linkage map produced from this study contained 1689 SNPs across 1196cM. The map was populated with an average of one SNP per 1.4 cM, spanning 11 linkage groups. Seed and flower color genes B and P were located on Pv02 and Pv07, respectively. A QTL for string: pod length (PL) ratio was found on Pv02 controlling 32% of total genetic variation. QTL for a suite of important processing traits including pod wall fiber, pod height, pod width, and pod wall thickness were found clustering on Pv04 and controlled 21%, 26%, 18%, and 16% of genetic variation for each of these respective traits. A QTL for PL was found on Pv09 controlling 5% of genetic variation. C1 [Hagerty, Christina H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Cuesta-Marcos, Alfonso] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Cregan, Perry; Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [McClean, Phil] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Myers, James R.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. RP Myers, JR (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. EM james.myers@oregonstate.edu FU Seneca Foods Corporation FX We are grateful for the assistance of Randy Hopson for his expertise in managing the field plots. We thank Deborah Kean, Joel Davis, Shawna Zimmerman, Kara Young, and Annie Chozinski of the Myers vegetable breeding group for their contributions in the field, greenhouse, and laboratory. Thanks are due to Ryan Graebner for valuable discussion. This research is part of an MS thesis by C.H. Hagerty and supported by a grant from Seneca Foods Corporation. CHH contributed to design, conducted experiments, and drafted the manuscript. AC-M assisted CHH with linkage map construction and QTL analysis. PC and QS developed the SNP platform used in the study and conducted allele calling of SNPs. PM contributed data from the Stampede x Redhawk RI population, with physical location of SNPs and gene annotation from the Phaseolus genome sequence. JRM conceived of the project, developed the recombinant inbred population, supervised the research and assisted in writing the publication. NR 40 TC 0 Z9 0 U1 3 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAR PY 2016 VL 141 IS 2 BP 131 EP 138 PG 8 WC Horticulture SC Agriculture GA DO9XJ UT WOS:000378141000005 ER PT J AU Schreiner, RP Scagel, CF AF Schreiner, R. Paul Scagel, Carolyn F. TI Arbuscule Frequency in Grapevine Roots Is More Responsive to Reduction in Photosynthetic Capacity Than to Increased Levels of Shoot Phosphorus SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article DE arbuscular mycorrhizal fungi; foliar fertilizer; root colonization; regulation; shading; Vitis vinifera ID VITIS-VINIFERA L.; MYCORRHIZAL FUNGI; MEDICAGO-TRUNCATULA; MESOCRICONEMA-XENOPLAX; LIGHT-INTENSITY; CARBON AVAILABILITY; GROWTH-RESPONSES; CORTICAL-CELLS; PLANT-GROWTH; PHOSPHATE AB An interplay between carbon and phosphorus is known to regulate root colonization by arbuscular mycorrhizal fungi (AMF); however, it is unclear whether plant C or plant P status plays a bigger role in controlling the abundance of arbuscules (the primary site of nutrient exchange in AMF symbiosis) in roots. In this study, 'Pinot noir' grapevine (Vitis vinifera) was grown in an unsterilized vineyard soil and colonized by indigenous AMF in two experiments, where photosynthetic capacity (defoliation or shading) and shoot nutrition (foliar fertilizer) were manipulated. Temporal changes in root colonization by AMF and plant growth and nutrition were determined. Foliar fertilizer application increased P and K uptake, but reduced Cu uptake in both experiments. Decreasing the photosynthetic capacity of shoots due to defoliation or shading rapidly reduced arbuscules in fine roots (within 7 to 14 days). In contrast a 3-fold increase in shoot P status from foliar fertilizer only reduced arbuscules after a more prolonged time (28 to 56 days). The combination of shading (15% of full sun) and foliar P application reduced arbuscules more than shading alone within the first month, whereas foliar P use in full sun had no influence on arbuscules within a month. Returning plants to full sun after 28 days in shade resulted in a resurgence of arbuscules in roots regardless of plant P status. Arbuscules in grapevine roots are regulated by the interaction between plant C and P status, such that high shoot P reduces arbuscule formation or maintenance more when combined with reduced plant photosynthesis. This indicates that grapevines do not reduce AMF nutrient transfer as an immediate response to elevated shoot P as long as plants are maintained in a high light environment. C1 [Schreiner, R. Paul; Scagel, Carolyn F.] USDA ARS, Hort Crops Res Lab, 3420 Northwest Orchard Ave, Corvallis, OR 97330 USA. RP Schreiner, RP (reprint author), USDA ARS, Hort Crops Res Lab, 3420 Northwest Orchard Ave, Corvallis, OR 97330 USA. EM paul.schreiner@ars.usda.gov NR 51 TC 0 Z9 0 U1 5 U2 8 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAR PY 2016 VL 141 IS 2 BP 151 EP 161 PG 11 WC Horticulture SC Agriculture GA DO9XJ UT WOS:000378141000008 ER PT J AU Wenk, ES Callaham, MA O'Brien, JJ Hanson, PJ AF Wenk, Evelyn S. Callaham, Mac A., Jr. O'Brien, Joseph J. Hanson, Paul J. TI Soil Macroinvertebrate Communities across a Productivity Gradient in Deciduous Forests of Eastern North America SO NORTHEASTERN NATURALIST LA English DT Article ID EXOTIC EARTHWORM INVASION; LITTER DECOMPOSITION; BIOLOGICAL INVASIONS; BOREAL FORESTS; PINE FORESTS; 1ST RECORDS; OLD-GROWTH; MILLIPEDES; MACROFAUNA; TEMPERATE AB Within the temperate, deciduous forests of the eastern US, diverse soil-fauna communities are structured by a combination of environmental gradients and interactions with other biota. The introduction of non-native soil taxa has altered communities and soil processes, and adds another degree of variability to these systems. We sampled soil macroinvertebrate abundance from forested sites in Missouri (MO), Michigan (MI), Massachusetts (MA), and New Hampshire (NH), with the objective of comparing community assemblages and evaluating the role of invasive earthworms along the temperature-productivity gradient represented by the sites. The primary detritivores encountered were earthworms and millipedes. Earthworms were collected only in MO and MI, and at much greater density in MO. Millipedes were found at every site except in MO, and at their highest mean density in NH. Warmer temperatures, higher litter productivity, and low Oa horizon depth (as found in MO) were correlated with high earthworm activity. Oa horizon depth was the greatest in NH, where the macroinvertebrate community was dominated (in terms of abundance) by predators and herbivores, not detritivores. Our results are suggestive of, and congruent with, the concept of earthworms as ecosystem engineers, as we found that the presence of non-native earthworm species was associated with significant differences in soil characteristics such as apparent rapid decomposition rates and reduced carbon storage in the Oa horizon. C1 [Wenk, Evelyn S.; Callaham, Mac A., Jr.; O'Brien, Joseph J.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, So Res Stn, 320 Green St, Athens, GA 30602 USA. [Hanson, Paul J.] Oak Ridge Natl Lab, Environm Sci Div, POB 2008, Oak Ridge, TN 37831 USA. RP Callaham, MA (reprint author), US Forest Serv, Ctr Forest Disturbance Sci, USDA, So Res Stn, 320 Green St, Athens, GA 30602 USA. EM mcallaham@fs.fed.us RI Hanson, Paul J./D-8069-2011 OI Hanson, Paul J./0000-0001-7293-3561 FU US Department of Energy, Office of Science, Office of Biological and Environmental Research through Oak Ridge National Laboratory; US Department of Energy [DE-AC05-00OR22725] FX Greta Langhenry, David Combs, Jim Le Moine, and Jasmine Crumsey assisted with field sampling. This material is based upon work supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research through a contract with Oak Ridge National Laboratory, which is managed by UT-Battelle, LLC, under Contract No. DE-AC05-00OR22725 with the US Department of Energy. NR 74 TC 1 Z9 1 U1 10 U2 19 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1092-6194 EI 1938-5307 J9 NORTHEAST NAT JI Northeast. Nat PD MAR PY 2016 VL 23 IS 1 BP 25 EP 44 PG 20 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DP0FG UT WOS:000378163600005 ER PT J AU Arruda, MP Brown, P Brown-Guedira, G Krill, AM Thurber, C Merrill, KR Foresman, BJ Kolb, FL AF Arruda, Marcio P. Brown, Patrick Brown-Guedira, Gina Krill, Allison M. Thurber, Carrie Merrill, Keith R. Foresman, Bradley J. Kolb, Frederic L. TI Genome-Wide Association Mapping of Fusarium Head Blight Resistance in Wheat using Genotyping-by-Sequencing SO PLANT GENOME LA English DT Article ID EUROPEAN WINTER-WHEAT; TRITICUM-AESTIVUM L.; SINGLE NUCLEOTIDE POLYMORPHISM; QTL ANALYSIS; LINKAGE DISEQUILIBRIUM; GENETIC ARCHITECTURE; FUNGAL SPREAD; SPRING WHEAT; DEOXYNIVALENOL; SELECTION AB Fusarium head blight (FHB) is one of the most important wheat (Triticum aestivum L.) diseases worldwide, and host resistance displays complex genetic control. A genome-wide association study (GWAS) was performed on 273 winter wheat breeding lines from the midwestern and eastern regions of the United States to identify chromosomal regions associated with FHB resistance. Genotyping-by-sequencing (GBS) was used to identify 19,992 single-nucleotide polymorphisms (SNPs) covering all 21 wheat chromosomes. Marker-trait associations were performed with different statistical models, the most appropriate being a compressed mixed linear model (cMLM) controlling for relatedness and population structure. Ten significant SNP-trait associations were detected on chromosomes 4A, 6A, 7A, 1D, 4D, and 7D, and multiple SNPs were associated with Fhb1 on chromosome 3B. Although combination of favorable alleles of these SNPs resulted in lower levels of severity (SEV), incidence (INC), and deoxynivalenol concentration (DON), lines carrying multiple beneficial alleles were in very low frequency for most traits. These SNPs can now be used for creating new breeding lines with different combinations of favorable alleles. This is one of the first GWAS using genomic resources from the International Wheat Genome Sequencing Consortium (IWGSC). C1 [Arruda, Marcio P.; Brown, Patrick; Krill, Allison M.; Foresman, Bradley J.; Kolb, Frederic L.] Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. [Brown-Guedira, Gina] ARS, USDA, Plant Sci Res Unit, 4114 Williams Hall, Raleigh, NC 27695 USA. [Thurber, Carrie] Abraham Baldwin Agr Coll, Sch Sci & Math, 2802 Moore Highway, Tifton, GA 31793 USA. [Merrill, Keith R.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Kolb, FL (reprint author), Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM f-kolb@illinois.edu FU United States Department of Agriculture [59-0206-9-080]; Triticeae-CAP - AFRI [2011-68002-30029]; Monsanto Company FX The authors are grateful to Dr. Yanhong Dong, from the University of Minnesota for deoxynivalenol determination. This material is based on work supported by the United States Department of Agriculture, under Agreement No. 59-0206-9-080. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. Marcio Arruda and Keith Merrill are grateful to Monsanto Company and the Triticeae-CAP (funded by AFRI grant No. 2011-68002-30029), for supporting their graduate studies, respectively. NR 59 TC 1 Z9 1 U1 17 U2 28 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2016 VL 9 IS 1 DI 10.3835/plantgenome2015.04.0028 PG 14 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DO9ZM UT WOS:000378146900005 ER PT J AU Hadasch, S Simko, I Hayes, RJ Ogutu, JO Piepho, HP AF Hadasch, Steffen Simko, Ivan Hayes, Ryan J. Ogutu, Joseph O. Piepho, Hans-Peter TI Comparing the Predictive Abilities of Phenotypic and Marker-Assisted Selection Methods in a Biparental Lettuce Population SO PLANT GENOME LA English DT Article ID GENOMIC PREDICTION; LA BRILLANTE; DOWNY MILDEW; RIDGE-REGRESSION; CUT LETTUCE; RESISTANCE; ACCURACY; INHERITANCE; VARIANCE; QUALITY AB Breeding for traits with polygenic inheritance is a challenging task that can be done by phenotypic selection, marker-assisted selection (MAS) or genome-wide selection. We comparatively evaluated the predictive abilities of four selection models on a biparental lettuce (Lactuca sativa L.) population genotyped with 95 single nucleotide polymorphisms and 205 amplified fragment length polymorphism markers. These models were based on (i) phenotypic selection, (ii) MAS (with quantitative trait locus (QTL)-linked markers), (iii) genomic prediction using all the available molecular markers, and (iv) genomic prediction using molecular markers plus QTL-linked markers as fixed covariates. Each model's performance was assessed using data on the field resistance to downy mildew (DMR, mean heritability similar to 0.71) and the quality of shelf life (SL, mean heritability similar to 0.91) of lettuce in multiple environments. The predictive ability of each selection model was computed under three cross-validation (CV) schemes based on sampling genotypes, environments, or both. For the DMR dataset, the predictive ability of the MAS model was significantly lower than that of the genomic prediction model. For the SL dataset, the predictive ability of the genomic prediction model was significantly lower than that for the model using QTL-linked markers under two of the three CV schemes. Our results show that the predictive ability of the selection models depends strongly on the CV scheme used for prediction and the heritability of the target trait. Our study also shows that molecular markers can be used to predict DMR and SL for individuals from this cross that were genotyped but not phenotyped. C1 [Hadasch, Steffen; Ogutu, Joseph O.; Piepho, Hans-Peter] Univ Hohenheim, Inst Crop Sci, Biostat Unit, Fruwirthstr 23, D-70599 Stuttgart, Germany. [Simko, Ivan; Hayes, Ryan J.] USDA ARS, Crop Improvement & Protect Res Unit, 1636 E Alisal St, Salinas, CA 93905 USA. RP Piepho, HP (reprint author), Univ Hohenheim, Inst Crop Sci, Biostat Unit, Fruwirthstr 23, D-70599 Stuttgart, Germany.; Simko, I (reprint author), USDA ARS, Crop Improvement & Protect Res Unit, 1636 E Alisal St, Salinas, CA 93905 USA. EM ivan.simko@ars.usda.gov; piepho@uni-hohenheim.de OI Ogutu, Joseph O/0000-0002-7379-0387 NR 32 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2016 VL 9 IS 1 DI 10.3835/plantgenome2015.03.0014 PG 11 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DO9ZM UT WOS:000378146900003 ER PT J AU Hofstad, AN Nussbaumer, T Akhunov, E Shin, S Kugler, KG Kistler, HC Mayer, KFX Muehlbauer, GJ AF Hofstad, Anna N. Nussbaumer, Thomas Akhunov, Eduard Shin, Sanghyun Kugler, Karl G. Kistler, H. Corby Mayer, Klaus F. X. Muehlbauer, Gary J. TI Examining the Transcriptional Response in Wheat Fhb1 Near-Isogenic Lines to Fusarium graminearum Infection and Deoxynivalenol Treatment SO PLANT GENOME LA English DT Article ID HEAD BLIGHT RESISTANCE; QUANTITATIVE TRAIT LOCI; TRITICUM-AESTIVUM L.; CHROMATOGRAPHY-MASS-SPECTROMETRY; INDUCED GENE-EXPRESSION; MYCOTOXIN DEOXYNIVALENOL; UDP-GLUCOSYLTRANSFERASE; ARABIDOPSIS-THALIANA; SCAB RESISTANCE; GIBBERELLA-ZEAE AB Fusarium head blight (FHB) is a disease caused predominantly by the fungal pathogen Fusarium graminearum that affects wheat and other small-grain cereals and can lead to severe yield loss and reduction in grain quality. Trichothecene mycotoxins, such as deoxynivalenol (DON), accumulate during infection and increase pathogen virulence and decrease grain quality. The Fhb1 locus on wheat chromosome 3BS confers Type II resistance to FHB and resistance to the spread of infection on the spike and has been associated with resistance to DON accumulation. To gain a better genetic understanding of the functional role of Fhb1 and resistance or susceptibility to FHB, we examined DON and ergosterol accumulation, FHB resistance, and the whole-genome transcriptomic response using RNA-seq in a near-isogenic line (NIL) pair carrying the resistant and susceptible alleles for Fhb1 during F. graminearum infection and DON treatment. Our results provide a gene expression atlas for the resistant and susceptible wheat-F. graminearum interaction. The DON concentration and transcriptomic results show that the rachis is a key location for conferring Type II resistance. In addition, the wheat transcriptome analysis revealed a set of Fhb1-responsive genes that may play a role in resistance and a set of DON-responsive genes that may play a role in trichothecene resistance. Transcriptomic results from the pathogen show that the F. graminearum genome responds differently to the host level of resistance. The results of this study extend our understanding of host and pathogen responses in the wheat-F. graminearum interaction. C1 [Hofstad, Anna N.; Shin, Sanghyun; Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Nussbaumer, Thomas; Kugler, Karl G.; Mayer, Klaus F. X.] Helmholtz Ctr Munich, Plant Genome & Syst Biol, D-85764 Neuherberg, Germany. [Nussbaumer, Thomas] Univ Vienna, Div Computat Syst Biol, Dept Microbiol & Ecosyst Sci, Waehringer Guertel 18, A-1090 Vienna, Austria. [Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Kistler, H. Corby] ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Muehlbauer, Gary J.] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. RP Muehlbauer, GJ (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA.; Muehlbauer, GJ (reprint author), Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA. EM muehl003@umn.edu RI Mayer, Klaus/M-7941-2015 OI Mayer, Klaus/0000-0001-6484-1077 FU Minnesota Wheat Research and Promotion Council; US Wheat and Barley Scab Initiative (USDA-ARS); University of Minnesota, Small Grains Initiative; Deutsche Forschungsgemeinschaft (DFG) [SFB 924]; Austrian Science Fund [F37] FX We thank the Minnesota Supercomputing Institute for computational support. We also thank Shane Heinen for technical help. This work was supported by funding from the Minnesota Wheat Research and Promotion Council, the US Wheat and Barley Scab Initiative (USDA-ARS), and the University of Minnesota, Small Grains Initiative. KFXM gratefully acknowledges funding by the Deutsche Forschungsgemeinschaft (DFG) SFB 924 and by the Austrian Science Fund special research project F37. NR 75 TC 1 Z9 1 U1 11 U2 15 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2016 VL 9 IS 1 DI 10.3835/plantgenome2015.05.0032 PG 15 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DO9ZM UT WOS:000378146900006 ER PT J AU Liu, ZB Cook, J Melia-Hancock, S Guill, K Bottoms, C Garcia, A Ott, O Nelson, R Recker, J Balint-Kurti, P Larsson, S Lepak, N Buckler, E Trimble, L Tracy, W McMullen, MD Flint-Garcia, SA AF Liu, Zhengbin Cook, Jason Melia-Hancock, Susan Guill, Katherine Bottoms, Christopher Garcia, Arturo Ott, Oliver Nelson, Rebecca Recker, Jill Balint-Kurti, Peter Larsson, Sara Lepak, Nicholas Buckler, Ed Trimble, Loren Tracy, William McMullen, Michael D. Flint-Garcia, Sherry A. TI Expanding Maize Genetic Resources with Predomestication Alleles: Maize-Teosinte Introgression Populations SO PLANT GENOME LA English DT Article ID QUANTITATIVE TRAIT LOCUS; NEAR-ISOGENIC LINES; ASSOCIATION MAPPING POPULATION; FLOWERING-TIME; MORPHOLOGICAL DIFFERENCES; POSTDOMESTICATION SPREAD; ARTIFICIAL SELECTION; DOMESTICATION; QTL; RICE AB Teosinte (Zea mays subsp. parviglumis H.H. Iltis & Doebley) has greater genetic diversity than maize inbreds and landraces (Z. mays subsp. mays). There are, however, limited genetic resources to efficiently evaluate and tap this diversity. To broaden resources for genetic diversity studies in maize, we developed and evaluated 928 near-isogenic introgression lines (NILs) from 10 teosinte accessions in the B73 background. Joint linkage analysis of the 10 introgression populations identified several large-effect quantitative trait loci (QTL) for days to anthesis (DTA), kernel row number (KRN), and 50-kernel weight (Wt50k). Our results confirm prior reports of kernel domestication loci and identify previously uncharacterized QTL with a range of allelic effects enabling future research into the genetic basis of these traits. Additionally, we used a targeted set of NILs to validate the effects of a KRN QTL located on chromosome 2. These introgression populations offer novel tools for QTL discovery and validation as well as a platform for initiating fine mapping. C1 [Liu, Zhengbin; Cook, Jason; McMullen, Michael D.; Flint-Garcia, Sherry A.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Melia-Hancock, Susan; Guill, Katherine; Bottoms, Christopher; Garcia, Arturo; Balint-Kurti, Peter; Lepak, Nicholas; Buckler, Ed; McMullen, Michael D.; Flint-Garcia, Sherry A.] Univ Missouri, USDA ARS, Informat Res Core Facil, Columbia, MO 65211 USA. [Ott, Oliver; Nelson, Rebecca] Cornell Univ, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Recker, Jill] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Balint-Kurti, Peter] N Carolina State Univ, Dept Plant Pathol, Box 7616, Raleigh, NC 27695 USA. [Larsson, Sara; Buckler, Ed] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14850 USA. [Buckler, Ed] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Trimble, Loren; Tracy, William] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Larsson, Sara] DuPont Pioneer, Windfall, IN 46076 USA. RP Flint-Garcia, SA (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA.; Flint-Garcia, SA (reprint author), Univ Missouri, USDA ARS, Informat Res Core Facil, Columbia, MO 65211 USA. EM Sherry.Flint-Garcia@ars.usda.gov FU National Science Foundation [IOS-0820619]; USDA-Agriculture Research Service FX We wish to thank AgReliant for the production of the doubled-haploid lines from the Z031 population. We also thank Nathan Springer and Natalia de Leon for planting the trial in Minnesota and Wisconsin and the McMullen, Flint-Garcia, Balint-Kurti, and Buckler groups for growing the trials, data collection, and providing ear and seed samples. This research was supported by National Science Foundation (IOS-0820619) and USDA-Agriculture Research Service. NR 53 TC 3 Z9 3 U1 12 U2 12 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD MAR PY 2016 VL 9 IS 1 DI 10.3835/plantgenome2015.07.0053 PG 11 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA DO9ZM UT WOS:000378146900010 ER PT J AU Bechere, E Zeng, L Hardin, RG AF Bechere, Efrem Zeng, L. Hardin, R. G. TI Combining Ability of Ginning Rate and Net Ginning Energy Requirement in Upland Cotton SO CROP SCIENCE LA English DT Article ID HOST-PLANT GERMPLASM; GOSSYPIUM-HIRSUTUM L; FIBER-QUALITY; LINT YIELD; F2 HYBRIDS; REGISTRATION; HETEROSIS; CULTIVARS AB Combining ability describes the breeding value of parental lines to produce hybrids. The objectives of this study were to estimate specific (SCA) and general combining ability (GCA) for ginning rate and net ginning energy requirement in a set of upland cotton (Gossypium hirsutum L.) germplasm. Ginning efficiency was based on measurements of ginning energy (watt hour [Wh] kg(-1) lint) and ginning rate (g lint s(-1)). There is little information in the scientific literature on this topic. Crosses were made in a 5. 8 factorial design between five female and eight male diverse cotton genotypes. The experiment was conducted in a randomized complete plot design. Analysis of variance was performed using a mixed model of the GLM procedures. Even though the study indicated the presence of both additive (GCA) and nonadditive (SCA) genes controlling these traits, a preponderance of the GCA effects was evident. The cotton genotype MD 25 had the highest GCA effect for ginning rate, and AR 9317-26 had the highest significant negative GCA effect for net ginning energy. For lowering fuzz percentage, any of the seminaked seed lines (AR 9317-26, Tejas NS, SC 9023 NS) can be exploited. This study and other previous studies showed significant positive correlation of fuzz percentage with net ginning energy. For cultivar development, cross JJ 1145ne x SC 9023 NS may be used for improving ginning rate, crosses FM 832. Tejas NS and JJ 1145ne x SG 747 can be used for reducing fuzz percentage, and the hybrid Phytogen 72 x TAM 98-99ne can be exploited for improving all three traits. C1 [Bechere, Efrem; Zeng, L.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Hardin, R. G.] USDA ARS, Cotton Ginning Res Unit, Stoneville, MS 38776 USA. RP Bechere, E (reprint author), USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM efrem.bechere@ars.usda.gov FU ARS [6066-21000-051-00D] FX This research is part of the National Program 301 (Plant Genetic Resources, Genomics and Genetic Improvement) and is funded through ARS Project Number 6066-21000-051-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 20 TC 1 Z9 1 U1 20 U2 20 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 499 EP 504 DI 10.2135/cropsci2015.05.0297 PG 6 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300001 ER PT J AU Pinson, SRM Jia, YL AF Pinson, Shannon R. M. Jia, Yulin TI QTLs for Early Tiller Production and Relationships with Rapid Seedling Growth and Increased Panicle Number in Rice SO CROP SCIENCE LA English DT Article ID QUANTITATIVE TRAIT LOCI; ORYZA-SATIVA L.; YIELD-RELATED TRAITS; DEVELOPMENTAL BEHAVIOR; AGRONOMIC TRAITS; PLANT HEIGHT; GENES; REGISTRATION; RESISTANCE; POPULATION AB Tiller number (TN) is a key determinant of final grain yield in the small grains crops, including rice (Oryza sativa L.), and is desired to enhance competition with weeds. Quantitative trait loci (QTLs) associated with TN were mapped within a set of recombinant inbred lines developed from a cross between the low-tillering tropical japonica cultivar 'Kaybon-net-lpa1-1' and the high-tillering, high-yielding, early-maturing indica cultivar 'Zhe733' using data collected from greenhouse potted plants evaluated weekly. Relationships between the five identified TN QTLs and related traits including panicle number (PN), flag leaf number, seedling leaf number (SLN), and earliness of tiller production were also evaluated. The three TN QTLs that were linked with early tiller emergence were also associated with PN; two additional TN not associated with PN were also identified. All five TN QTLs were also SLN QTLs, though other loci affecting SLN were also identified. Several of the TN and SLN QTLs identified using data from young plants (e.g., = 6 wk after planting) were not detectible in data collected just before or after heading. The three TN QTLs also associated with PN showed stronger effects (higher logarithm of the odds ratios and additive effects) on TN as observed in seedling stages than on final TN at heading time, and their effects diminished over time to the point that they were not always detectible in TN data collected at or after heading. However, the three TN QTLs that were also PN QTLs were robust enough that they were detected in the less intensive (one-fourth the labor and resources) Arkansas trials, where tillers were counted from two single-plant replications just twice, at 6 wk after planting and after heading. The three loci associated with both TN and PN are the TN loci most likely to increase rice yield, and the markers presently found linked to them can be used for marker assisted breeding. C1 [Pinson, Shannon R. M.; Jia, Yulin] USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Highway 130 East, Stuttgart, AR 72160 USA. RP Pinson, SRM (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Highway 130 East, Stuttgart, AR 72160 USA. EM shannon.pinson@ars.usda.gov NR 68 TC 0 Z9 0 U1 13 U2 19 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 505 EP 519 DI 10.2135/cropsci2014.09.0667 PG 15 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300002 ER PT J AU Chung, YS Palta, J Bamberg, J Jansky, S AF Chung, Yong Suk Palta, Jiwan Bamberg, John Jansky, Shelley TI Potential Molecular Markers Associated with Tuber Calcium Content in Wild Potato Germplasm SO CROP SCIENCE LA English DT Article ID SOFT-ROT; CATION/H+ EXCHANGERS; INTERNAL QUALITY; ARABIDOPSIS; PLANTS; ACCUMULATION; TRANSPORT; GENE; IDENTIFICATION; CONSTRUCTION AB High tuber Ca is associated with a reduced incidence of disease and physiological disorders in potato (Solanum tuberosum L.). However, genetic variation for tuber Ca content in cultivated potato is low, limiting opportunities to study the genetic basis of this trait. We used wild germplasm to develop a population segregating for tuber Ca concentration. The high-Ca-accumulating potato wild relative S. microdontum Bitter (clone M15) was crossed to low-Ca-accumulating S. kurtzianum Bitter & Wittm. (clone K12) and 12 F-1 individuals were intercrossed to create a segregating population. Significant variation in tuber Ca content was found in this population, and simple sequence repeats (SSRs) were tested for association with tuber Ca content. Twelve of 42 SSRs tested were associated with tuber Ca content. SSR4743 on chromosome 7 was found to be linked to a cation exchanger-like (CAX3-like) gene known to be involved with Ca uptake in plants. This SSR may be used to develop a molecular marker to identify tubers with high Ca levels. C1 [Chung, Yong Suk; Palta, Jiwan] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Bamberg, John; Jansky, Shelley] Univ Wisconsin, USDA ARS, Dept Hort, Madison, WI 53706 USA. RP Jansky, S (reprint author), Univ Wisconsin, USDA ARS, Dept Hort, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov FU USDA-NRI [2009-55605-05219] FX Funding for this project was provided by USDA-NRI Grant 2009-55605-05219. NR 54 TC 0 Z9 0 U1 3 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 576 EP 584 DI 10.2135/cropsci2015.06.0370 PG 9 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300008 ER PT J AU Thompson, AL Smiley, RW Paulitz, TC Garland-Campbell, K AF Thompson, Alison L. Smiley, Richard W. Paulitz, Timothy C. Garland-Campbell, Kim TI Identification of Resistance to Pratylenchus neglectus and Pratylenchus thornei in Iranian Landrace Accessions of Wheat SO CROP SCIENCE LA English DT Article ID ROOT-LESION NEMATODE; TRITICUM-AESTIVUM L.; PLANT GENETIC-RESOURCES; QUANTITATIVE TRAIT LOCI; STRIPE RUST RESISTANCE; BREAD WHEAT; PUCCINIA-STRIIFORMIS; PACIFIC-NORTHWEST; GREEN-REVOLUTION; SPRING WHEAT AB The pathogenic nematodes Pratylenchus neglectus (Rensch, 1924) Filipjev and Schuurmans Stekhoven, 1941 and Pratylenchus thornei Sher and Allen, 1953 cause severe yield losses in wheat (Triticum aestivum L.). The objectives in this study were to assay a collection of Iranian landrace accessions collected from 12 provinces in Iran to identify novel sources of resistance to both species and to characterize agronomic traits critical for consideration in wheat breeding. Seventy-eight accessions were assayed for dual resistance to parasitic nematodes P. neglectus and P. thornei in controlled environment assays. Field trials conducted in Pullman, WA, and Pendleton, OR, evaluated stripe rust (Puccinia striiformis f. sp. tritici) resistance, days to heading, grain volume weight, plant height, seed protein content, seed kernel characterization, glume tenacity, and pubescence. The accessions were assayed with simple-sequence repeat (SSR), single-nucleotide polymorphism (SNP), and known vernalization markers for hierarchical cluster analysis to identify relatedness among accessions. Thirty-two accessions were identified as resistant or moderately resistant to both Pratylenchus species. Six were identified with moderate adult plant resistance to stripe rust in the field. The range of mean agronomic traits over locations was 53 to 105 cm for plant height, 46 to 84 d for post planting days to heading, and 151 to 728 kg m(-3) for grain volume weight. The genetic cluster analysis identified three clusters based on the number of rare polymorphisms in the subset. The nematode resistance was distributed over the three clusters. The diversity within this subset could be useful for wheat breeders to integrate genetic variation and resistance to both Pratylenchus spp. C1 [Thompson, Alison L.] USDA, Plant Physiol & Genet Res Unit, 21881 Cardon Lane, Maricopa, AZ 85138 USA. [Smiley, Richard W.] Oregon State Univ, Columbia Basin Agr Res Ctr, POB 370, Pendleton, OR 97801 USA. [Paulitz, Timothy C.] Washington State Univ, Root Dis & Biol Control Res Unit, ARS, USDA, 363 Johnson Hall, Pullman, WA 99164 USA. [Garland-Campbell, Kim] Washington State Univ, Wheat Genet Qual & Physiol Dis Res Unit, ARS, USDA, 209 Johnson Hall, Pullman, WA 99164 USA. RP Thompson, AL (reprint author), USDA, Plant Physiol & Genet Res Unit, 21881 Cardon Lane, Maricopa, AZ 85138 USA. EM Alison.Thompson@ars.usda.gov FU Washington Grains Commission [3949]; USDA-ARS [5348-21220-003-00D, 5348-22000-013-00]; Washington State University O. A Vogel grant [8334] FX We thank the funding agencies for this project: the Washington Grains Commission project no. 3949, USDA-ARS (in house) projects, 5348-21220-003-00D and 5348-22000-013-00, and the Washington State University O. A Vogel grant 8334; Jason Sheedy for providing advice on working with these landrace accessions and Pratylenchus; the Garland-Campbell summer field crew, Jocelyn Bowser, Miles Hansen, Anna Campbell, and Tricia DeMacon managed by Stephen Johnson for help with the field assessments; Doug Engle for help with the SKCS measurements; Adrienne Burke for help with the seedling stripe rust and nematode assays; Anmin Wan of Dr. Xianmeng Chen's research group, and members of the Smiley research team, Jennifer Gourlie and Dr. Guiping Yan for providing P. neglectus inoculum. NR 76 TC 0 Z9 0 U1 5 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 654 EP 672 DI 10.2135/cropsci2015.07.0438 PG 19 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300015 ER PT J AU Springer, TL Goldman, JJ AF Springer, T. L. Goldman, J. J. TI Germination of Saccharum ravennae (L.) L. (Poaceae) Caryopses and Intact Spikelets SO CROP SCIENCE LA English DT Article ID CHAFFY-SEEDED GRASSES; GROWTH AB Ravennagrass, Saccharum ravennae (L.) L. [synonym: Erianthus ravennae (Linn.) P. Beauv.], is a robust perennial bunchgrass grown as an ornamental in the US, but, where adapted, has become naturalized in riparian areas. Little is known about the seed size distribution and germination characteristics of ravennagrass caryopses or intact spikelets. The objectives of this study were to determine, for two populations: (i) the caryopsis size distribution, (ii) the germination of sized caryopses, (iii) the length of seedling shoots and roots from caryopses of different sizes, (iv) the germination of unsized caryopses (UC) and intact spikelets (IS), and (v) the length of seedling shoots and roots from UC and IS. Although seed production of ravennagrass is relatively low, under favorable conditions, ravennagrass can produce more than 10,000 caryopses per panicle. Caryopses germinated well within 14 d and averaged >80% germination; however, caryopses germinated >90% if their mass was >0.3 mg. Intact spikelets were slower to germinate in 14 d and had a lower percentage of abnormally germinated seed compared with caryopses. Intact spikelets also had a significantly higher percentage of firm seeds compared with caryopses and the total potential germination of IS was greater than caryopses exceeding 90%. Understanding the germination characteristics of ravennagrass will aid in the development of seeding rates and the processing of seeds for agronomic uses. C1 [Springer, T. L.; Goldman, J. J.] USDA ARS, Southern Plains Range Res Stn, Woodward, OK 73801 USA. RP Springer, TL (reprint author), USDA ARS, Southern Plains Range Res Stn, Woodward, OK 73801 USA. EM tim.springer@ars.usda.gov NR 25 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 682 EP 688 DI 10.2135/cropsci2014.11.0781 PG 7 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300017 ER PT J AU Thom, MD Eberle, CA Forcella, F Gesch, R Weyers, S Lundgren, JG AF Thom, Matthew D. Eberle, Carrie A. Forcella, Frank Gesch, Russ Weyers, Sharon Lundgren, Jonathan G. TI Nectar Production in Oilseeds: Food for Pollinators in an Agricultural Landscape SO CROP SCIENCE LA English DT Article ID COLONY COLLAPSE DISORDER; MASS-FLOWERING CROPS; WILD BEE ABUNDANCE; CAMELINA-SATIVA; CORN-BELT; POLLEN; OIL; CONSERVATION; HONEYBEES; DECLINES AB Pollinating insects are in decline throughout the world, driven by a combination of factors including the loss of forage resources. The maize (Zea mays L.)-and soybean [Glycine max (L.) Merr.]-dominated agriculture of the Central and Midwestern United States produces a landscape relatively devoid of nectar and pollen resources. Introducing specialty oilseeds into current crop rotations could provide abundant floral resources for pollinating insects as well as a high-value crop for growers. We investigated the nectar sugar resources and insect visitation throughout flower anthesis of nine specialty oilseed crops in west-central Minnesota and eastern South Dakota during the 2013 and 2014 growing seasons. Total sugar produced over anthesis (TS) was highest for echium (Echium plantagineum L.) at 472 kg ha(-1). Canola (Brassica napus L.), crambe (Crambe abyssinica Hochst.), echium, borage (Borago officinalis L.), and cuphea (Cuphea viscosissima Jacq. x Cuphea lanceolata W. T. Aiton) produced enough sugar in one hectare to supply the annual sugar needs of a least one managed honey bee (Apis mellifera L.) colony. Pollinators visited flowers of all crops, with as many as 90 insects min(-1) observed. Our study is unique as we measured nectar sugar production, flower density, and insect visitation throughout anthesis for multiple specialty oilseed crops, providing a seasonwide perspective of the flux of nectar resources for pollinators. Adding specialty oilseed crops into current crop rotations could aid in reversing pollinator decline by providing forage resources that are lacking in the current agricultural landscape. C1 [Thom, Matthew D.; Eberle, Carrie A.; Forcella, Frank; Gesch, Russ; Weyers, Sharon] USDA ARS, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. [Lundgren, Jonathan G.] USDA ARS, North Cent Agr Lab, 2923 Medary Ave, Brookings, SD 57006 USA. RP Thom, MD (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM matt.thom@ars.usda.gov FU U.S. Department of Agriculture National Institute of Food and Agriculture [2012-67009-20272]; Minnesota Honey Producers Association FX We want to thank the U.S. Department of Agriculture National Institute of Food and Agriculture award 2012-67009-20272 for funding of this research, USDA Agricultural Research Service National Program Staff, and the moral support from the Minnesota Honey Producers Association. We personally thank C. Hennen, S. Larson, and D. Schneider for establishing and maintaining the farm research plots; G. Smith, J. Oldakowski, and D. Danforth for help in the field; J. Rinke for help in the lab and with HPLC; J. Eklund and D. Peterson for considerable assistance in the design and execution of this work; and N. Price and M. Bowman for additional analysis. We also thank the four anonymous reviewers whose input helped to improve the manuscript. NR 60 TC 1 Z9 2 U1 19 U2 30 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 727 EP 739 DI 10.2135/cropsci2015.05.0322 PG 13 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300022 ER PT J AU Salmeron, M Gbur, EE Bourland, FM Buehring, NW Earnest, L Fritschi, FB Golden, BR Hathcoat, D Lofton, J McClure, AT Miller, TD Neely, C Shannon, G Udeigwe, TK Verbree, DA Vories, ED Wiebold, WJ Purcell, LC AF Salmeron, Montserrat Gbur, Edward E. Bourland, Fred M. Buehring, Normie W. Earnest, Larry Fritschi, Felix B. Golden, Bobby R. Hathcoat, Daniel Lofton, Josh McClure, Angela Thompson Miller, Travis D. Neely, Clark Shannon, Grover Udeigwe, Theophilus K. Verbree, David A. Vories, Earl D. Wiebold, William J. Purcell, Larry C. TI Yield Response to Planting Date Among Soybean Maturity Groups for Irrigated Production in the US Midsouth SO CROP SCIENCE LA English DT Article ID CROPPING SYSTEM; ENVIRONMENTAL-CONDITIONS; BIOMASS RESPONSES; UNITED-STATES; GROWTH; SEED; TEMPERATURE; PHOTOPERIOD; POPULATION AB Planting date is one of the main factors affecting soybean (Glycine max [L.] Merr.) yield. Environmental conditions in the US Midsouth allow for planting dates from late March through early July, and maturity groups (MGs) ranging from 3 to 6. However, the complexity of interactions among planting date, MG, and the environment makes the selection of an optimum MG cultivar difficult. A regional 3-yr study, conducted at eight locations with latitudes ranging from 30.6 to 38.9 degrees N, planting dates ranging from late March to early July, and MGs 3 to 6, was used to examine the relationship between relative yield and planting day. The data indicated that yield was dependent on the location and MG choice. There was a quadratic response of relative yield to planting day in six out of the eight locations studied for MG 3 cultivars, and in five locations for MG 4 cultivars. On the other hand, MG 5 and 6 cultivars were more likely to have a negative linear relationship, with a quadratic response in only two of the eight locations. Optimum planting dates that maximized yield were dependent on the location and MG combination and ranged from 22 March to 17 May. Delaying planting dates from mid May to early June reduced yields by 0.09 to 1.69% per day, with the rate of decline greatest at the southern-most locations. Overall, MG 4 cultivars maximized yield or were not statistically different from the highest yielding MG at most locations and planting dates. C1 [Salmeron, Montserrat; Purcell, Larry C.] Univ Arkansas, Dept Crop Soil & Environ Sci, 1366 W Altheimer Dr, Fayetteville, AR 72704 USA. [Gbur, Edward E.] Univ Arkansas, Agr Stat Lab, 101 Agr Annex, Fayetteville, AR 72701 USA. [Bourland, Fred M.] Univ Arkansas, Northeast Res & Extens Ctr, 1241 W Cty Rd 780, Keiser, AR 72351 USA. [Buehring, Normie W.] Mississippi State Univ, North Mississippi Res & Extens Ctr, 5421 Highway 145 South, Verona, MS 38879 USA. [Earnest, Larry] Univ Arkansas, Southeast Branch Expt Stn, 140 Expt Stn Loop, Watson, AR 71674 USA. [Fritschi, Felix B.; Wiebold, William J.] Univ Missouri, Div Plant Sci, 1-31 Agr Bldg, Columbia, MO 65211 USA. [Golden, Bobby R.] Mississippi State Univ, Delta Res & Extens Ctr, POB 197,82 Stoneville Rd, Stoneville, MS 38776 USA. [Lofton, Josh] Oklahoma State Univ, 376 Agr Hall, Stillwater, OK 74078 USA. [Hathcoat, Daniel; Miller, Travis D.; Neely, Clark] Texas A&M, Texas AgriLife Extens Serv, 370 Olsen Blvd, College Stn, TX 77843 USA. [Shannon, Grover] Univ Missouri, 147 State Hwy T, Portageville, MO 63873 USA. [Vories, Earl D.] USDA ARS, Fisher Delta Res Ctr, 147 State Hwy T, Portageville, MO 63873 USA. [Udeigwe, Theophilus K.] Texas Tech Univ, Dept Plant & Soil Sci, 2907 15th St, Lubbock, TX 79409 USA. [McClure, Angela Thompson; Verbree, David A.] Univ Tennessee, Dept Plant Sci, West TN AgRes Ctr, 605 Airways Blvd, Jackson, TN 38301 USA. RP Purcell, LC (reprint author), Univ Arkansas, Dept Crop Soil & Environ Sci, 1366 W Altheimer Dr, Fayetteville, AR 72704 USA. EM lpurcell@uark.edu FU United Soybean Board; Mid-South Soybean Board FX The authors gratefully acknowledge partial support for this research from the United Soybean Board and the Mid-South Soybean Board. NR 33 TC 3 Z9 3 U1 7 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAR-APR PY 2016 VL 56 IS 2 BP 747 EP 759 DI 10.2135/cropsci2015.07.0466 PG 13 WC Agronomy SC Agriculture GA DO3XG UT WOS:000377715300024 ER PT J AU Shemshura, ON Bekmakhanova, NE Mazunina, MN Meyer, SLF Rice, CP Masler, EP AF Shemshura, Olga N. Bekmakhanova, Nadiya E. Mazunina, Mariya N. Meyer, Susan L. F. Rice, Clifford P. Masler, Edward P. TI Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE bio-based compounds; dimethyl citrate isomer; fungal toxins; natural products; nematotoxicity; spectral identification ID PLANT-PARASITIC NEMATODES; CITRIC-ACID FERMENTATION; PRATYLENCHUS-PENETRANS; MELOIDOGYNE-INCOGNITA; NIGER AB Culture medium from an isolate of the fungus Aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. Column, thin layer and preparative chromatographies and spectral and elemental analyses, were used to isolate and identify two major constituents of an active fraction (Fraction F) obtained from the medium. Compound 1 was identified as 2-hydroxypropane-1, 2, 3-tricarboxylic acid (citric acid). Compound 2 was identified as 3-hydroxy-5-methoxy-3-(methoxycarbonyl)-5-oxopentanoic acid, an isomer of 1, 2-dimethyl citrate. Compound 1 and a citric acid standard, each tested at 50 mg mL(-1) in water, decreased hatch from eggs of the plant-parasitic nematode Meloidogyne incognita by more than 94%, and completely immobilized second-stage juveniles after 4-6 days exposure. Fraction F and Compounds 1 and 2 decreased the mobility of adults of the plant-parasitic nematode Ditylenchus destructor in vitro. Fraction F (25 mg mL(-1)) inhibited mobility >99% at 72 hrs. Compounds 1 and 2 (50 mg mL(-1)) each inhibited mobility more than 25% at 24 hr and more than 50% at 72 hr. This is the first assignment of nematode-antagonistic properties to specifically identified A. candidus metabolites. C1 [Shemshura, Olga N.; Bekmakhanova, Nadiya E.; Mazunina, Mariya N.] Minist Educ & Sci Republ Kazakhstan, Inst Microbiol & Virol, Bogenbai Batyr St 103, Alma Ata 050510, Kazakhstan. [Meyer, Susan L. F.; Masler, Edward P.] ARS, Nematol Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Rice, Clifford P.] ARS, Sustainable Agr Syst Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Masler, EP (reprint author), USDA ARS, Nematol Lab, Nematol, 10300 Baltimore Ave,B-010A, Beltsville, MD 20705 USA. EM edward.masler@ars.usda.gov FU ISTC FX We thank the ISTC for financial support to NEB and ONS. NR 29 TC 1 Z9 1 U1 4 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0378-1097 EI 1574-6968 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD MAR PY 2016 VL 363 IS 5 AR fnw026 DI 10.1093/femsle/fnw026 PG 9 WC Microbiology SC Microbiology GA DO7ML UT WOS:000377966600009 PM 26850440 ER PT J AU Li, SX Song, QJ Martins, AM Cregan, P AF Li, Shuxian Song, Qijian Martins, Alexandre M. Cregan, Perry TI Draft genome sequence of Diaporthe aspalathi isolate MS-SSC91, a fungus causing stem canker in soybean SO GENOMICS DATA LA English DT Article DE Draft genome; Diaporthe aspalathi; Stem Canker; Sequences; Soybean pathogen ID PHOMOPSIS-LONGICOLLA; PHASEOLORUM; GENES AB Diaporthe aspalathi (Syn. Diaporthe phaseolorum var. meridionalis) is the causal agent of the southern stem canker (SSC) disease in soybean. This disease can kill plants from the middle to the end of the growing season resulting in severe yield loss. The mechanisms of SSC disease development and pathogen invasion of soybean are not fully understood. The genome sequence of D. aspalathi has not been described. In this article, we report the successful assembly of the draft genome sequence of a D. aspalathi isolate, designated MS-SSC91, that was isolated from the stem of a field-grown soybean plant in Mississippi, USA in 2006. This study represents the first reported genome sequence of D. aspalathi in the Diaporthe-Phomopsis complex. The whole genome shotgun sequence of the MS-SSC91 isolate has been deposited at DDBJ/EMBL/GenBank under the accession LJJS00000000 and the sequences could be found at the site http://www.ncbi.nlm.nih.gov/assembly/GCA_001447215.1/. The MS-SSC91 genome sequences will provide information on the genetic basis of fungal infection of the soybean stem. It is valuable for studying soybean-fungal interactions and developing new control strategies for this pathogen. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). C1 [Li, Shuxian] ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Song, Qijian; Martins, Alexandre M.; Cregan, Perry] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Li, SX (reprint author), ARS, USDA, Crop Genet Res Unit, Stoneville, MS 38776 USA. EM shuxian.li@ars.usda.gov NR 10 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2213-5960 J9 GENOM DATA JI Genom. Data PD MAR PY 2016 VL 7 BP 262 EP 263 DI 10.1016/j.gdata.2016.02.002 PG 2 WC Genetics & Heredity SC Genetics & Heredity GA DO4NY UT WOS:000377761100077 PM 26981423 ER PT J AU Tanaka, K Arita, S AF Tanaka, Kiyoyasu Arita, Shawn TI The impact of regional investment liberalization on foreign direct investment: A firm-level simulation assessment SO JAPAN AND THE WORLD ECONOMY LA English DT Article DE Regional integration; Investment liberalization; FDI; Firm heterogeneity; Japan ID ECONOMIC-INTEGRATION; MULTINATIONAL FIRMS; EAST-ASIA; TRADE; FDI; STRATEGIES; LOCATION; WELFARE AB With the proliferation of regional economic integration, investment liberalization has gained in importance. This paper conducts counterfactual policy experiments to simulate the response of multinational firms to a regional decline in investment costs. We find evidence of investment creation effects at the aggregate level. While there is weak evidence of investment diversion effects, the non-participation in regional integration discourages multinational activity of non-member countries. As less productive firms experience the extensive-margin growth strongly, the effects differ significantly by multinational firms with heterogeneous productivity. (C) 2016 Elsevier B.V. All rights reserved. C1 [Tanaka, Kiyoyasu] JETRO, Inst Dev Econ, Mihama Ku, 3-2-2 Wakaba, Chiba 2618545, Japan. [Arita, Shawn] USDA, Econ Res Serv, 355 E St SW, Washington, DC USA. RP Tanaka, K (reprint author), JETRO, Inst Dev Econ, Mihama Ku, 3-2-2 Wakaba, Chiba 2618545, Japan. EM kiyoyasu_tanaka@ide.go.jp; ssarita@ers.usda.gov FU JSPS [26285058, 24730232] FX This study is conducted as a part of the project "Economic Analysis on Trade Agreements" undertaken at the Research Institute of Economy, Trade and Industry (RIETI). We acknowledge the financial support of JSPS Grant-in-Aid for Scientific Research (B) Grant Number 26285058 and Young Scientists (B) Grant Number 24730232. The authors would like to thank RIETI for research opportunities. For their useful comments, we thank Hitoshi Sato, Hikari Ishido, Tadashi Ito, Isao Kamata, Yoshimasa Komoriya, Hiroshi Mukunoki, and seminar participants at Tohoku University. The opinions expressed and arguments employed in this paper are the sole responsibility of the authors and do not necessarily reflect those of RIETI, METI, the Economic Research Service, the United States Department of Agriculture, or any institution with which the authors are affiliated. All remaining errors are our own. NR 27 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0922-1425 EI 1879-2006 J9 JPN WORLD ECON JI Jpn. World Econ. PD MAR-MAY PY 2016 VL 37-38 BP 17 EP 26 DI 10.1016/j.japwor.2016.02.001 PG 10 WC Economics SC Business & Economics GA DO5TF UT WOS:000377845000003 ER PT J AU Chehoud, C Dryga, A Hwang, Y Nagy-Szakal, D Hollister, EB Luna, RA Versalovic, J Kellermayer, R Bushman, FD AF Chehoud, Christel Dryga, Anatoly Hwang, Young Nagy-Szakal, Dorottya Hollister, Emily B. Luna, Ruth Ann Versalovic, James Kellermayer, Richard Bushman, Frederic D. TI Transfer of Viral Communities between Human Individuals during Fecal Microbiota Transplantation SO MBIO LA English DT Article ID HUMAN GUT VIROME; VIRUSES; DISEASE; INFECTION; FECES; HIV-1; DIET; CELL AB Fecal microbiota transplantation (FMT) is a highly effective treatment for refractory Clostridium difficile infections. However, concerns persist about unwanted cotransfer of pathogenic microbes such as viruses. Here we studed FMT from a single healthy human donor to three pediatric ulcerative colitis patients, each of whom received a course of 22 to 30 FMT treatments. Viral particles were purified from donor and recipient stool samples and sequenced; the reads were then assembled into contigs corresponding to viral genomes or partial genomes. Transfer of selected viruses was confirmed by quantitative PCR. Viral contigs present in the donor could be readily detected in recipients, with up to 32 different donor viral contigs appearing in a recipient sample. Reassuringly, none of these were viruses are known to replicate on human cells. Instead, viral contigs either scored as bacteriophage or could not be attributed taxonomically, suggestive of unstudied phage. The two most frequently transferred gene types were associated with temperate-phage replication. In addition, members of Siphoviridae, the group of typically temperate phages that includes phage lambda, were found to be transferred with significantly greater efficiency than other groups. On the basis of these findings, we propose that the temperate-phage replication style may promote efficient phage transfer between human individuals. In summary, we documented transfer of multiple viral lineages between human individuals through FMT, but in this case series, none were from viral groups known to infect human cells. IMPORTANCE Transfer of whole communities of viruses between humans has rarely been studied but is of likely medical importance. Here we studied fecal microbiota transplantation (FMT), a highly successful treatment for relapsing Clostridium difficile infection and, potentially, other gastrointestinal (GI) diseases. We investigated the transfer of viral communities during FMT and documented transfer of multiple viral lineages between humans. None of these were viruses that replicated on animal cells or that are known to be pathogenic. We found that temperate bacteriophage, which form stable associations with their hosts, were significantly more likely to be transferred during FMT. This supports a model in which the viral temperate replication style may have evolved in part to support efficient viral transmission between environments. C1 [Chehoud, Christel; Dryga, Anatoly; Hwang, Young; Bushman, Frederic D.] Univ Penn, Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA. [Dryga, Anatoly] St Petersburg State Univ, Dept Chem, St Petersburg 199034, Russia. [Nagy-Szakal, Dorottya; Kellermayer, Richard] Texas Childrens Hosp, Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Sect Pediat Gastroenterol,Dept Pediat, Houston, TX 77030 USA. [Hollister, Emily B.; Luna, Ruth Ann; Versalovic, James] Texas Childrens Hosp, Dept Pathol, Texas Childrens Microbiome Ctr, Houston, TX 77030 USA. [Hollister, Emily B.; Versalovic, James] Baylor Coll Med, Dept Pathol & Immunol, Houston, TX 77030 USA. RP Bushman, FD (reprint author), Univ Penn, Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA. EM bushman@upenn.edu FU PennCHOP Microbiome Program; Gutsy Kids Fund; HHS \ National Institutes of Health (NIH) [P30 AI045008, AI082020, T32 AI007632, R01 AT004326, UH3 DK083990, U01 CA170930, P30 DK56338] FX This work, including the efforts of Frederic D. Bushman, was funded by PennCHOP Microbiome Program. This work, including the efforts of Richard Kellermayer, was funded by Gutsy Kids Fund. This work, including the efforts of Frederic D. Bushman, was funded by HHS vertical bar National Institutes of Health (NIH) (P30 AI045008 and AI082020). This work, including the efforts of Christel Chehoud, was funded by HHS vertical bar National Institutes of Health (NIH) (T32 AI007632). This work, including the efforts of James Versalovic, was funded by HHS vertical bar National Institutes of Health (NIH) (R01 AT004326, UH3 DK083990, U01 CA170930, and P30 DK56338). NR 25 TC 8 Z9 9 U1 4 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD MAR-APR PY 2016 VL 7 IS 2 AR e00322-16 DI 10.1128/mBio.00322-16 PG 8 WC Microbiology SC Microbiology GA DO4QT UT WOS:000377768700018 PM 27025251 ER PT J AU Engel, P Kwong, WK McFrederick, Q Anderson, KE Barribeau, SM Chandler, JA Cornman, RS Dainat, J de Miranda, JR Doublet, V Emery, O Evans, JD Farinelli, L Flenniken, ML Granberg, F Grasis, JA Gauthier, L Hayer, J Koch, H Kocher, S Martinson, VG Moran, N Munoz-Torres, M Newton, I Paxton, RJ Powell, E Sadd, BM Schmid-Hempel, P Schmid-Hempel, R Song, SJ Schwarz, RS Vanengelsdorp, D Dainat, B AF Engel, Philipp Kwong, Waldan K. McFrederick, Quinn Anderson, Kirk E. Barribeau, Seth Michael Chandler, James Angus Cornman, R. Scott Dainat, Jacques de Miranda, Joachim R. Doublet, Vincent Emery, Olivier Evans, Jay D. Farinelli, Laurent Flenniken, Michelle L. Granberg, Fredrik Grasis, Juris A. Gauthier, Laurent Hayer, Juliette Koch, Hauke Kocher, Sarah Martinson, Vincent G. Moran, Nancy Munoz-Torres, Monica Newton, Irene Paxton, Robert J. Powell, Eli Sadd, Ben M. Schmid-Hempel, Paul Schmid-Hempel, Regula Song, Se Jin Schwarz, Ryan S. vanengelsdorp, Dennis Dainat, Benjamin TI The Bee Microbiome: Impact on Bee Health and Model for Evolution and Ecology of Host-Microbe Interactions SO MBIO LA English DT Review ID HONEYBEE APIS-MELLIFERA; DEFORMED-WING-VIRUS; PATHOGENS NOSEMA-CERANAE; COLONY COLLAPSE DISORDER; GUT MICROBIOTA; BUMBLE BEES; DROSOPHILA-MELANOGASTER; DEFENSE-MECHANISMS; DIVERSITY; INFECTION AB As pollinators, bees are cornerstones for terrestrial ecosystem stability and key components in agricultural productivity. All animals, including bees, are associated with a diverse community of microbes, commonly referred to as the micro biome. The bee micro biome is likely to be a crucial factor affecting host health. However, with the exception of a few pathogens, the impacts of most members of the bee microbiome on host health are poorly understood. Further, the evolutionary and ecological forces that shape and change the microbiome are unclear. Here, we discuss recent progress in our understanding of the bee microbiome, and we present challenges associated with its investigation. We conclude that global coordination of research efforts is needed to fully understand the complex and highly dynamic nature of the interplay between the bee micro biome, its host, and the environment. High-throughput sequencing technologies are ideal for exploring complex biological systems, including host-microbe interactions. To maximize their value and to improve assessment of the factors affecting bee health, sequence data should be archived, curated, and analyzed in ways that promote the synthesis of different studies. To this end, the BeeBiome consortium aims to develop an online database which would provide reference sequences, archive metadata, and host analytical resources. The goal would be to support applied and fundamental research on bees and their associated microbes and to provide a collaborative framework for sharing primary data from different research programs, thus furthering our understanding of the bee microbiome and its impact on pollinator health. C1 [Engel, Philipp; Emery, Olivier; Gauthier, Laurent; Dainat, Benjamin] Univ Lausanne, Dept Fundamental Microbiol, Lausanne, Switzerland. [Kwong, Waldan K.; Gauthier, Laurent] Yale Univ, Ecol & Evolutionary Biol, New Haven, CT USA. [Kwong, Waldan K.; Koch, Hauke; Moran, Nancy; Powell, Eli] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. [McFrederick, Quinn] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Anderson, Kirk E.] USDA, Carl Hayden Bee Res Ctr, Tucson, AZ USA. [Barribeau, Seth Michael] E Carolina Univ, Dept Biol, Greenville, NC USA. [Chandler, James Angus] Calif Acad Sci, Dept Microbiol, San Francisco, CA 94118 USA. [Cornman, R. Scott] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO USA. [Dainat, Jacques] Linkopings Univ Victoria Westling, BILS, Linkoping, Sweden. [Dainat, Jacques] Uppsala Univ, Dept Med Biochem & Microbiol, Uppsala, Sweden. [de Miranda, Joachim R.] Swedish Univ Agr Sci, Dept Ecol, Uppsala, Sweden. [Doublet, Vincent; Paxton, Robert J.] Univ Halle Wittenberg, Inst Biol, D-06108 Halle, Germany. [Doublet, Vincent; Paxton, Robert J.] German Ctr Integrat Biodivers Res iDiv, Leipzig, Germany. [Evans, Jay D.; Schwarz, Ryan S.] ARS, USDA, Bee Res Lab, Beltsville, MD USA. [Farinelli, Laurent] Fasteris SA, Plan Les Ouates, Switzerland. [Flenniken, Michelle L.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Granberg, Fredrik] BVF, SLU, Uppsala, Sweden. [Grasis, Juris A.] San Diego State Univ, Dept Biol, North Life Sci, San Diego, CA 92182 USA. [Hayer, Juliette] SLU, Inst Husdjursgenet, Uppsala, Sweden. [Koch, Hauke] Royal Bot Gardens, Richmond, Surrey, England. [Kocher, Sarah] Harvard Univ, Dept Organism & Evolutionary Biol, Museum Comparat Zool, Cambridge, MA 02138 USA. [Martinson, Vincent G.] Univ Rochester, Dept Biol, Rochester, NY 14627 USA. [Munoz-Torres, Monica] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA. [Newton, Irene] Indiana Univ, Dept Biol, Bloomington, IN USA. [Sadd, Ben M.] Illinois State Univ, Sch Biol Sci, Normal, IL 61761 USA. [Schmid-Hempel, Paul; Schmid-Hempel, Regula] ETHZ Inst Integrat Biol, Zurich, Switzerland. [Song, Se Jin] Univ Colorado, Boulder, CO 80309 USA. [vanengelsdorp, Dennis] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Dainat, Benjamin] Swiss Bee Resegman Ctr, Bern, Switzerland. [Dainat, Benjamin] Apiservice, Bee Hlth Extens Serv, Bern, Switzerland. [Chandler, James Angus; Dainat, Benjamin] Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA. RP Engel, P (reprint author), Univ Lausanne, Dept Fundamental Microbiol, Lausanne, Switzerland.; Dainat, B (reprint author), Apiservice, Bee Hlth Extens Serv, Bern, Switzerland.; Dainat, B (reprint author), Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA. EM philipp.engel@unil.ch; benjamin.dainat@agroscope.admin.ch RI Paxton, Robert/D-7082-2015; Doublet, Vincent/D-8050-2015; Granberg, Fredrik/F-2325-2014; OI Paxton, Robert/0000-0003-2517-1351; Doublet, Vincent/0000-0002-6313-1222; Granberg, Fredrik/0000-0001-5497-9611; Evans, Jay/0000-0002-0036-4651; Grasis, Juris/0000-0002-3945-0135; Rodrigues de Miranda, Joachim/0000-0002-0335-0386; Sadd, Ben/0000-0003-3136-5144 FU National Evolutionary Synthesis Center (NESCent), NSF [EF-0905606] FX This manuscript arose from the discussions by the Bee Microbiome consortium at the National Evolutionary Synthesis Center (NESCent) workshop "BeeBiome:Omic approaches for understanding bee microbe relationships" held in Durham, NC, USA from 20 to 24th October 2014. The workshop was supported by the National Evolutionary Synthesis Center (NESCent), grant NSF #EF-0905606 to B.D., P.E., J.R.M., J.D.E., and L.G. NR 100 TC 8 Z9 8 U1 32 U2 56 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD MAR-APR PY 2016 VL 7 IS 2 AR e02164 DI 10.1128/mBio.02164-15 PG 9 WC Microbiology SC Microbiology GA DO4QT UT WOS:000377768700029 PM 27118586 ER PT J AU Johnson, TA Stedtfeld, RD Wang, Q Cole, JR Hashsham, SA Looft, T Zhu, YG Tiedje, JM AF Johnson, Timothy A. Stedtfeld, Robert D. Wang, Qiong Cole, James R. Hashsham, Syed A. Looft, Torey Zhu, Yong-Guan Tiedje, James M. TI Clusters of Antibiotic Resistance Genes Enriched Together Stay Together in Swine Agriculture SO MBIO LA English DT Article ID CLASS 1 INTEGRONS; REAL-TIME PCR; MULTIDRUG-RESISTANCE; INTESTINAL BACTERIA; INSERTION SEQUENCES; FOOD ANIMALS; SOIL; CASSETTES; PLASMIDS; ANTIMICROBIALS AB Antibiotic resistance is a worldwide health risk, but the influence of animal agriculture on the genetic context and enrichment of individual antibiotic resistance alleles remains unclear. Using quantitative PCR followed by amplicon sequencing, we quantified and sequenced 44 genes related to antibiotic resistance, mobile genetic elements, and bacterial phylogeny in microbiomes from U.S. laboratory swine and from swine farms from three Chinese regions. We identified highly abundant resistance clusters: groups of resistance and mobile genetic element alleles that cooccur. For example, the abundance of genes conferring resistance to six classes of antibiotics together with class 1 integrase and the abundance of IS6100-type transposons in three Chinese regions are directly correlated. These resistance cluster genes likely colocalize in microbial genomes in the farms. Resistance cluster alleles were dramatically enriched (up to 1 to 10% as abundant as 16S rRNA) and indicate that multidrug-resistant bacteria are likely the norm rather than an exception in these communities. This enrichment largely occurred independently of phylogenetic composition; thus, resistance clusters are likely present in many bacterial taxa. Furthermore, resistance clusters contain resistance genes that confer resistance to antibiotics independently of their particular use on the farms. Selection for these clusters is likely due to the use of only a subset of the broad range of chemicals to which the clusters confer resistance. The scale of animal agriculture and its wastes, the enrichment and horizontal gene transfer potential of the clusters, and the vicinity of large human populations suggest that managing this resistance reservoir is important for minimizing human risk. IMPORTANCE Agricultural antibiotic use results in clusters of cooccurring resistance genes that together confer resistance to multiple antibiotics. The use of a single antibiotic could select for an entire suite of resistance genes if they are genetically linked. No links to bacterial membership were observed for these clusters of resistance genes. These findings urge deeper understanding of colocalization of resistance genes and mobile genetic elements in resistance islands and their distribution throughout antibiotic-exposed microbiomes. As governments seek to combat the rise in antibiotic resistance, a balance is sought between ensuring proper animal health and welfare and preserving medically important antibiotics for therapeutic use. Metagenomic and genomic monitoring will be critical to determine if resistance genes can be reduced in animal microbiomes, or if these gene clusters will continue to be coselected by antibiotics not deemed medically important for human health but used for growth promotion or by medically important antibiotics used therapeutically. C1 [Johnson, Timothy A.; Stedtfeld, Robert D.; Wang, Qiong; Cole, James R.; Hashsham, Syed A.; Tiedje, James M.] Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA. [Johnson, Timothy A.; Tiedje, James M.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Stedtfeld, Robert D.; Hashsham, Syed A.] Michigan State Univ, Dept Civil & Environm Engn, E Lansing, MI 48824 USA. [Zhu, Yong-Guan] Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen, Peoples R China. [Zhu, Yong-Guan] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, POB 934, Beijing, Peoples R China. [Johnson, Timothy A.; Looft, Torey] ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Tiedje, JM (reprint author), Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA.; Tiedje, JM (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. EM tiedjej@msu.edu RI Zhu, Yong-Guan/A-1412-2009; CAS, KLUEH/G-8978-2016; CAS, KLUEH-Cooperation/E-1148-2017 OI Zhu, Yong-Guan/0000-0003-3861-8482; FU Pharmaceuticals in the Environment Initiative at Michigan State University; Center for Health Impacts of Agriculture at Michigan State University; Center for Microbial Ecology at Michigan State University; Office of Science (Biological and Environmental Research), U.S. Department of Energy [DE-FG02-99ER62848]; Natural Science Foundation of China [21210008] FX Funding was provided by the Pharmaceuticals in the Environment Initiative, the Center for Health Impacts of Agriculture and the Center for Microbial Ecology, all at Michigan State University. QW and JRC were supported by the Office of Science (Biological and Environmental Research), U.S. Department of Energy [DE-FG02-99ER62848]. YGZ was supported by the Natural Science Foundation of China (No. 21210008). NR 73 TC 9 Z9 9 U1 14 U2 20 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD MAR-APR PY 2016 VL 7 IS 2 AR e02214-15 DI 10.1128/mBio.02214-15 PG 11 WC Microbiology SC Microbiology GA DO4QT UT WOS:000377768700044 PM 27073098 ER PT J AU Schreiber, LA Hansen, CP Rumble, MA Millspaugh, JJ Thompson, FR Gamo, RS Kehmeier, JW Wojik, N AF Schreiber, Leslie A. Hansen, Christopher P. Rumble, Mark A. Millspaugh, Joshua J. Thompson, Frank R., III Gamo, R. Scott Kehmeier, Jon W. Wojik, Nate TI Greater sage-grouse apparent nest productivity and chick survival in Carbon County, Wyoming SO WILDLIFE BIOLOGY LA English DT Article ID PLANT-CHEMICAL DEFENSES; BROOD-REARING HABITAT; SHARP-TAILED GROUSE; CENTROCERCUS-UROPHASIANUS; CENTRAL WASHINGTON; CLIMATE-CHANGE; NORTH-AMERICA; POPULATIONS; SELECTION; DYNAMICS AB Greater sage-grouse Centrocercus urophasianus populations across North America have been declining due to degradation and fragmentation of sagebrush habitat. As part of a study quantifying greater sage-grouse demographics prior to construction of a wind energy facility, we estimated apparent net nest productivity and survival rate of chicks associated with radio-equipped female sage-grouse in Carbon County, Wyoming, USA. We estimated apparent net nest productivity using a weighted mean of the average brood size and used a modified logistic-exposure method to estimate daily chick survival over a 70-day time period. Apparent nest productivity was 2.79 chicks per female (95% CI: 1.46-4.12) in 2011, 2.00 chicks per female (95% CI: 1.00-3.00) in 2012, and 1.54 chick per female (95% CI: 0.62-2.46) in 2013. Chick survival to 70 days post-hatch was 19.10% (95% CI: 6.22-37.42%) in 2011, 4.20% (95% CI: 0.84-12.31%) in 2012, and 16.05% (95% CI: 7.67-27.22%) in 2013. These estimates were low, yet within the range of other published survival rates. Chick survival was primarily associated with year and chick age, with minor effects of average temperature between surveys and hatch date. The variability in chick survival rates across years of our study suggests annual weather patterns may have large impacts on chick survival. Thus, management actions that increase the availability of food and cover for chicks may be necessary, especially during years with drought and above-average spring temperatures. C1 [Schreiber, Leslie A.; Hansen, Christopher P.; Millspaugh, Joshua J.] Univ Missouri, Sch Nat Resources, 103 ABNR Bldg, Columbia, MO 65211 USA. [Rumble, Mark A.] US Forest Serv, Rocky Mt Res Stn, 8221 South Highway 16, Rapid City, SD 57702 USA. [Thompson, Frank R., III] Univ Missouri, USDA, Forest Serv, No Res Stn, 202 ABNR Bldg, Columbia, MO 65211 USA. [Gamo, R. Scott] Wyoming Game & Fish Dept, 5400 Bishop Ave, Cheyenne, WY 82006 USA. [Kehmeier, Jon W.; Wojik, Nate] SWCA Environm Consultants, 295 Interlocken Blvd Suite 300, Broomfield, CO 80021 USA. RP Millspaugh, JJ (reprint author), Univ Missouri, Sch Nat Resources, 103 ABNR Bldg, Columbia, MO 65211 USA. EM millspaughj@missouri.edu FU U.S. Forest Service Rocky Mountain Research Station; Wyoming Game and Fish Dept; Power Company of Wyoming LLC; Bureau of Land Management; U.S. Dept of Energy National Renewable Energy Laboratory; Western Association of Fish and Wildlife Agencies; National Fish and Wildlife Foundation; SWCA Environmental Consultants; Overland Trail Cattle Company, LLC FX The research associated with this publication resulted from research funded, in part, by U.S. Forest Service Rocky Mountain Research Station, Wyoming Game and Fish Dept, Power Company of Wyoming LLC, Bureau of Land Management, U.S. Dept of Energy National Renewable Energy Laboratory, Western Association of Fish and Wildlife Agencies, National Fish and Wildlife Foundation, and SWCA Environmental Consultants. The National Wind Coordinating Collaborative and American Wind Wildlife Institute helped to acquire funds for our research program focused on sage-grouse ecology and wind energy development. A. Fremgen and many field technicians assisted with sage-grouse tagging, tracking and associated field work. We particularly thank G. Miller, Vice President of Land and Environmental Affairs, Power Company of Wyoming, LLC and the Overland Trail Cattle Company, LLC for their support of the project and assistance with field activities. NR 77 TC 1 Z9 1 U1 2 U2 4 PU WILDLIFE BIOLOGY PI RONDE PA C/O JAN BERTELSEN, GRENAAVEJ 14, KALO, DK-8410 RONDE, DENMARK SN 0909-6396 EI 1903-220X J9 WILDLIFE BIOL JI Wildlife Biol. PD MAR PY 2016 VL 22 IS 2 BP 37 EP 44 DI 10.2981/wlb.00124 PG 8 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DO5DK UT WOS:000377803600002 ER PT J AU Lonsinger, RC Gese, EM Knight, RN Johnson, TR Waits, LP AF Lonsinger, Robert C. Gese, Eric M. Knight, Robert N. Johnson, Timothy R. Waits, Lisette P. TI Quantifying and correcting for scat removal in noninvasive carnivore scat surveys SO WILDLIFE BIOLOGY LA English DT Article ID MODEL SELECTION; ABUNDANCE; DECAY; TIME; IDENTIFICATION; PERSISTENCE; PARAMETERS; OCCUPANCY; SURVIVAL; SAMPLES AB Scat surveys are commonly used to monitor wildlife populations. For carnivores, surveys are typically conducted along roads and trails. Scats available for detection may not reflect scats deposited and variation in disappearance may bias results. Previous research has investigated natural decay and deterioration, but scats deposited along roads or trails are likely influenced to a greater degree by anthropogenic disturbance in some systems. We used experimental plots to evaluate variation in scat removal for two model carnivores, coyote Canis latrans and kit fox Vulpes macrotis, along roads in the Great Basin Desert, USA. Using parametric survival regression, we predicted scat survival and developed persistence-rate correction factors, which were applied to results from relative abundance scat surveys conducted along 15 transects. Kit fox scats disappeared more rapidly than coyote scats, with 3.3% and 10.6%, respectively, persisting through 42 days. At 14 days, 90.8-41.7% of scats had been removed across road types. Survival models indicated species, road type, scat position and daily traffic were important predictors of scat persistence. Applying persistence-rate correction factors to scat survey results altered the inferred relative abundances. Across seasons, mean corrected: uncorrected relative abundance ratios ranged from 1.0-91.2 for coyotes and 1.3-139.3 for kit foxes, with higher mean ratios being influenced by high corrected relative abundances on roads with high traffic volumes. Understanding scat removal rates and patterns can improve inferences from surveys. Persistence-rate correction factors can be used to reduce bias in indices of abundance, but caution should be used when removal rates are high. Knowledge of spatial variation in persistence can elucidate concerns of false-positives and false-negatives in occupancy and capture-recapture studies. Considering the disparity in scat removal between species and among road types and positions, we recommend practitioners quantify and consider variation in removal when interpreting scat survey results. C1 [Lonsinger, Robert C.; Waits, Lisette P.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Gese, Eric M.] Utah State Univ, USDA, Dept Wildland Resources, Natl Wildlife Res Ctr,Wildlife Serv, Logan, UT 84322 USA. [Knight, Robert N.] US Army Dugway Proving Ground, Nat Resource Program, Dugway, UT 84022 USA. [Johnson, Timothy R.] Univ Idaho, Dept Stat Sci, Moscow, ID 83844 USA. RP Lonsinger, RC (reprint author), Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. EM lons1663@vandals.uidaho.edu FU US Dept of Defense's Environmental Security Technology Certification Program [12 EB-RC5-006]; Legacy Resource Management Program [W9132T-12-2-0050] FX Funding was provided by the US Dept of Defense's Environmental Security Technology Certification Program (12 EB-RC5-006) and Legacy Resource Management Program (W9132T-12-2-0050). The US Army Dugway Proving Ground Natural Resource Management Program provided traffic counters. The Utah Division of Wildlife Resources provided housing. T. Edwards, B. Kluever, K. Marvel, M. Melham and M. Smith assisted with data collection. B. Cypher and D. Richardson helped with obtaining kit fox scats. K. Rayner and M. Wright provided traffic data. NR 36 TC 2 Z9 2 U1 5 U2 8 PU WILDLIFE BIOLOGY PI RONDE PA C/O JAN BERTELSEN, GRENAAVEJ 14, KALO, DK-8410 RONDE, DENMARK SN 0909-6396 EI 1903-220X J9 WILDLIFE BIOL JI Wildlife Biol. PD MAR PY 2016 VL 22 IS 2 BP 45 EP 54 DI 10.2981/wlb.00179 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DO5DK UT WOS:000377803600003 ER PT J AU Centofanti, T McConnell, LL Chaney, RL Beyer, WN Andrade, NA Hapeman, CJ Torrents, A Nguyen, A Anderson, MO Novak, JM Jackson, D AF Centofanti, Tiziana McConnell, Laura L. Chaney, Rufus L. Beyer, W. Nelson Andrade, Natasha A. Hapeman, Cathleen J. Torrents, Alba Anh Nguyen Anderson, Marya O. Novak, Jeffrey M. Jackson, Dana TI Organic amendments for risk mitigation of organochlorine pesticide residues in old orchard soils SO ENVIRONMENTAL POLLUTION LA English DT Article DE Bioavailability; DDT; Dieldrin; Soil; Earthworms ID AGRICULTURAL SOIL; EISENIA-FOETIDA; FOOD-CHAIN; BIOAVAILABILITY; POLLUTANTS; ACCUMULATION; EARTHWORM; BIOCHAR; IMPACT; CARBON AB Performance of compost and biochar amendments for in situ risk mitigation of aged DDT, DDE and dieldrin residues in an old orchard soil was examined. The change in bioavailability of pesticide residues to Lumbricus terrestris L. relative to the unamended control soil was assessed using 4-L soil microcosms with and without plant cover in a 48-day experiment. The use of aged dairy manure compost and biosolids compost was found to be effective, especially in the planted treatments, at lowering the bioavailability factor (BAF) by 18-39%; however, BAF results for DDT in the unplanted soil treatments were unaffected or increased. The pine chip biochar utilized in this experiment was ineffective at lower the BAF of pesticides in the soil. The US EPA Soil Screening Level approach was used with our measured values. Addition of 10% of the aged dairy manure compost reduced the average hazard quotient values to below 1.0 for DDT + DDE and dieldrin. Results indicate this sustainable approach is appropriate to minimize risks to wildlife in areas of marginal organochlorine pesticide contamination. Application of this remediation approach has potential for use internationally in areas where historical pesticide contamination of soils remains a threat to wildlife populations. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Centofanti, Tiziana; Andrade, Natasha A.; Torrents, Alba; Anderson, Marya O.] Univ Maryland, Dept Civil & Environm Engn, 1173 Glenn L Martin Hall, College Pk, MD 20742 USA. [McConnell, Laura L.; Chaney, Rufus L.; Hapeman, Cathleen J.; Anh Nguyen; Jackson, Dana] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Beyer, W. Nelson] US Geol Survey, Patuxent Wildlife Res Ctr, 10300 Baltimore Ave,Bldg 308,BARC East, Beltsville, MD 20705 USA. [Novak, Jeffrey M.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 West Lucas St, Florence, SC USA. [Centofanti, Tiziana] Calif State Univ Fresno, Ctr Irrigat Technol, Fresno, CA 93740 USA. RP McConnell, LL (reprint author), Bayer CropSci, 2 TW Alexander Dr, Res Triangle Pk, NC 27709 USA. EM tiziana.centofanti@gmail.com; laura.mcconnell@bayer.com OI Centofanti, Tiziana/0000-0002-5545-4064 FU U.S. Environmental Protection Agency, Region III Green Remediation Program FX We acknowledge contributions from Mr. Christopher Jennings, and Dr. Carrie Green of USDA-ARS, Beltsville, Maryland, USA and Dr. Ken Cantrell, formerly of USDA-ARS, Florence, South Carolina, USA for creation of the biochar material used in this study. Funding for this study was received from the U.S. Environmental Protection Agency, Region III Green Remediation Program. NR 40 TC 0 Z9 0 U1 7 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD MAR PY 2016 VL 210 BP 182 EP 191 DI 10.1016/j.envpol.2015.11.039 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM9RO UT WOS:000376703600022 PM 26716732 ER PT J AU Choi, YE Lee, C Goodwin, SB AF Choi, Yoon-E Lee, Changsu Goodwin, Stephen B. TI Generation of Reactive Oxygen Species via NOXa Is Important for Development and Pathogenicity of Mycosphaerella graminicola SO MYCOBIOLOGY LA English DT Article DE AREA; CREA; Development; Mycosphaerella graminicola; NADPH oxidase; NOXa; Over-expression; Pathogenicity; ROS; Septoria tritici blotch; Zymoseptoria tritici ID NITROGEN METABOLITE REPRESSION; CATABOLITE REPRESSION; ASPERGILLUS-NIDULANS; SEPTORIA-TRITICI; RESISTANT WHEAT; NADPH OXIDASES; DISEASE; FUNGUS; MECHANISMS; INFECTION AB The ascomycete fungus Mycosphaerella graminicola (synonym Zymoseptoria tritici) is an important pathogen of wheat causing economically significant losses. The primary nutritional mode of this fungus is thought to be hemibiotrophic. This pathogenic lifestyle is associated with an early biotrophic stage of nutrient uptake followed by a necrotrophic stage aided possibly by production of a toxin or reactive oxygen species (ROS). In many other fungi, the genes CREA and AREA are important during the biotrophic stage of infection, while the NOXa gene product is important during necrotrophic growth. To test the hypothesis that these genes are important for pathogenicity of M. graminicola, we employed an over-expression strategy for the selected target genes CREA, AREA, and NOXa, which might function as regulators of nutrient acquisition or ROS generation. Increased expressions of CREA, AREA, and NOXa in M. graminicola were confirmed via quantitative real-time PCR and strains were subsequently assayed for pathogenicity. Among them, the NOXa over-expression strain, NO2, resulted in significantly increased virulence. Moreover, instead of the usual filamentous growth, we observed a predominance of yeast-like growth of NO2 which was correlated with ROS production. Our data indicate that ROS generation via NOXa is important to pathogenicity as well as development in M. graminicola. C1 [Choi, Yoon-E] Korea Univ, Div Environm Sci & Ecol Engn, Seoul 02841, South Korea. [Lee, Changsu] Chonbuk Natl Univ, Dept Bioproc Engn, Jeonju 54896, South Korea. [Goodwin, Stephen B.] Purdue Univ, USDA ARS, Dept Bot & Plant Pathol, 915 West State St, W Lafayette, IN 47907 USA. RP Goodwin, SB (reprint author), Purdue Univ, USDA ARS, Dept Bot & Plant Pathol, 915 West State St, W Lafayette, IN 47907 USA. EM Steve.Goodwin@ARS.USDA.gov FU USDA-ARS CRIS [3602-22000-015-00D, 3602-22000-017-00D]; USDA-ARS; Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2014R1A1A2055300] FX We thank Drs. Larry Dunkle and Kristin Laluk for critical reading of the manuscript. We also thank Dr. Ian Thompson for assistance in the pathogenicity experiment. This work was supported by USDA-ARS CRIS projects 3602-22000-015-00D and 3602-22000-017-00D and by a USDA-ARS Administrator-Funded Postdoctoral position to Y.-E. Choi. This research was also financially supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2014R1A1A2055300). NR 32 TC 0 Z9 0 U1 2 U2 4 PU KOREAN SOC MYCOLOGY PI ANAM-DEONG, SEONGBUK-GU PA KOREAN UNIV, COLL LIFE SCIENCES & BIOTECHNOLOGY, 427 WEST BUILDING, ANAM-DEONG, SEONGBUK-GU, SEOUL 136-701, SOUTH KOREA SN 1229-8093 EI 2092-9323 J9 MYCOBIOLOGY JI Mycobiology PD MAR PY 2016 VL 44 IS 1 BP 38 EP 47 DI 10.5941/MYCO.2016.44.1.38 PG 10 WC Agronomy; Mycology SC Agriculture; Mycology GA DN3XQ UT WOS:000376997900006 PM 27103853 ER PT J AU Hofmeyr, JH Hofmeyr, M Carpenter, JE Bloem, S Slabbert, JP AF Hofmeyr, J. H. Hofmeyr, M. Carpenter, J. E. Bloem, S. Slabbert, J. P. TI Sterile insect releases for control of Thaumatotibia leucotreta (Lepidoptera: Tortricidae): an assessment on semi-commercial scale SO AFRICAN ENTOMOLOGY LA English DT Article DE false codling moth; SIT; pilot project ID FALSE CODLING MOTH; TRICHOGRAMMATIDAE; CITRUS AB False codling moth, Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae), is indigenous to sub-Saharan Africa. It infests a large number of wild plants and can be a pest of commercial concern in cultivated fruit and nuts. A limited number of insecticides, as well as biological control and mating disruption can be applied as control measures in citrus, but they often do not reduce pre- and post-harvest crop loss adequately. The potential of the sterile insect technique (SIT) for the control of T. leucotreta was investigated between 2002 and 2006 in Citrusdal, Western Cape Province, South Africa, and entailed studies on radiation biology and Fl sterility, compatibility with the egg parasitoid Trichogrammatoidea Nagaraja (Hymenoptera: Trichogrammatidae), and field cage experiments to determine sterile:fertile moth overflooding ratios. Promising results justified a pilot experiment conducted on semi-commercial scale in citrus orchards in Citrusdal during 2005-06. One thousand mixed sex moths were treated with 150 Gy ionising radiation and released in 35 ha of Washington navel oranges twice a week for 28 weeks during the production season. The moths were released by hand from an all-terrain vehicle, with release rows 40 m apart and the numbers of released and feral moths monitored weekly with pheromone traps. Released moths dispersed well and fully overlapped between release rows. The target overflooding ratio of released to feral males (10:1) was exceeded by wide margins and a mean overflooding ratio of 41:1 was maintained during the experiment. Fruit drop assessments were conducted once a week in the SIT-treated and control orchards. Crop loss due to T leucotreta infestation was reduced by 95.2 % in the SIT-treated area compared to the control orchard. The project resulted in the commercialisation of the SIT for T leucotreta on citrus. C1 [Hofmeyr, J. H.; Hofmeyr, M.] Citrus Res Int, POB 212, ZA-7340 Citrusdal, South Africa. [Carpenter, J. E.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Bloem, S.] USDA APHIS PPQ CPHST, Plant Epidemiol & Risk Anal Lab, Raleigh, NC USA. [Slabbert, J. P.] IThemba Lab Accelerator Based Sci, Somerset West, Western Cape Pr, South Africa. RP Hofmeyr, JH (reprint author), Citrus Res Int, POB 212, ZA-7340 Citrusdal, South Africa. EM jhh9345@gmail.com FU International Atomic Energy Agency FX The International Atomic Energy Agency is thanked for their financial and technical support enabling the establishment of much of the infrastructure necessary for the SIT research. B. Barnes (ARC Infruitec, Stellenbosch, retired) reviewed this paper comprehensively. We sincerely appreciate the time and effort he spent to iron out the wrinkles. The Visser family of Hexrivier Sitrus in Citrusdal, Western Cape Province, made available their Blikhuis farm for experimental purposes. We thank them for use of the site, as well as for managing the farming operations in such a way that it added value to our research. S. Honiball (formerly with Ceder Biocontrol, Citrusdal, Western Cape Province) was responsible for rearing sufficient numbers of T. leucotreta for the SIT project. We sincerely acknowledge his dedication in not once failing to meet production deadlines, enabling moth releases to continue without interruption. We gratefully acknowledge the contributions of B. Barnes, L. Arnold and J. Johnson, Infruitec Research Institute, Agricultural Research Council, Stellenbosch, Western Cape Province, for allowing us unlimited access to their cobalt-60 irradiator, and lending technical assistance whenever required. We thank Seyfert Corrugated Western Cape (Pty) Ltd for the donation of ample, indispensable supplies of SFK cardboard used in the T. leucotreta rearing procedure. NR 20 TC 1 Z9 1 U1 7 U2 7 PU ENTOMOLOGICAL SOC SOUTHERN AFRICA PI HATFIELD PA PO BOX 13162, HATFIELD 0028, SOUTH AFRICA SN 1021-3589 EI 1026-4914 J9 AFR ENTOMOL JI Afr. Entomol. PD MAR PY 2016 VL 24 IS 1 BP 80 EP 89 DI 10.4001/003.024.0080 PG 10 WC Entomology SC Entomology GA DM5VU UT WOS:000376419200009 ER PT J AU Miller, DR Giliomee, JH AF Miller, D. R. Giliomee, J. H. TI Taxonomic revision of the South African mealybug genus Octococcus Hall (Hemiptera: Pseudococcidae) SO AFRICAN ENTOMOLOGY LA English DT Article DE Octococcus; Pseudococcidae; mealybugs; immatures; new species; South Africa ID HOMOPTERA; COCCOIDEA AB A taxonomic revision of the South African mealybug genus Octococcus Hall is presented. Most species of this genus feed on plants in the Asteraceae. Six species are treated, namely, O. barbarae, O. gullanae. and O. warniae which are described as new. The three previously described species are: Octococcus africanus (Brain), O. minor De Lotto, and O. pentziae Hall. Octococcus salsolicola (Priensner & Hosny), which was described originally as Ripersia salsolicola, is treated as a nomen dubium. Adult females of all species are described and illustrated and a key for their identification is presented. Illustrations and descriptions are also presented of first-instar nymphs of Octococcus africanus, O. minor, and O. warniae and a key for their identification is presented. Descriptions and illustrations are also given for a second-instar nymph of O. africanus and a third-instar nymph of O. warniae. C1 [Miller, D. R.] USDA ARS, Systemat Entomol Lab, Beltsville, MD USA. [Miller, D. R.] Dept Plant Ind, Gainesville, FL USA. [Giliomee, J. H.] Univ Stellenbosch, Ctr Invas Biol, Dept Bot & Zool, Private Bag X1, ZA-7602 Matieland, South Africa. RP Giliomee, JH (reprint author), Univ Stellenbosch, Ctr Invas Biol, Dept Bot & Zool, Private Bag X1, ZA-7602 Matieland, South Africa. EM jhg@sun.ac.za NR 25 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOLOGICAL SOC SOUTHERN AFRICA PI HATFIELD PA PO BOX 13162, HATFIELD 0028, SOUTH AFRICA SN 1021-3589 EI 1026-4914 J9 AFR ENTOMOL JI Afr. Entomol. PD MAR PY 2016 VL 24 IS 1 BP 112 EP 142 DI 10.4001/003.024.0112 PG 31 WC Entomology SC Entomology GA DM5VU UT WOS:000376419200012 ER PT J AU Fill, JM Moule, BM Varner, JM Mousseau, TA AF Fill, Jennifer M. Moule, Brett M. Varner, J. Morgan Mousseau, Timothy A. TI Flammability of the keystone savanna bunchgrass Aristida stricta SO PLANT ECOLOGY LA English DT Article DE Fire behavior; Fuel structure; Pine savanna; Sandhills; Wiregrass ID FIRE BEHAVIOR; BURNING CHARACTERISTICS; GRASS INVASION; PINE SAVANNAS; FREQUENT FIRE; FOREST; FUELS; LITTER; COMMUNITIES; VEGETATION AB Understanding the flammability of species in fire-prone or fire-dependent ecosystems is necessary for modeling and predicting ecosystem dynamics. Wiregrass (Aristida stricta syn. A. beyrichiana), a keystone perennial bunchgrass, is a dominant groundcover species in southeastern United States pine savannas. Although wiregrass flammability as a driver of pine savanna fire regimes is a fundamental paradigm in pine savanna dynamics, no studies have quantified its fuel structure and flammability at the individual bunchgrass level. We studied wiregrass flammability at the Aiken Gopher Tortoise Heritage Preserve in Aiken County, South Carolina, USA. We linked tussock fuel structure characteristics (total biomass, live: dead biomass, mass of perched litter and pine needles, moisture content, and bulk density) to flammability (flaming duration, smoldering duration, and flame length). Flame length was strongly and positively related towiregrass biomass. Pine needles and other litter fuels perched on wiregrass tussocks were not related to flame length, but increased the duration of flaming and smoldering. Within the ranges evaluated, neither fire weather (relative humidity, wind speed, and air temperature) nor fuel moisture significantly affected tussock flammability. Our results indicate that different fuel structural properties drive separate aspects of wiregrass flammability. Together with litter from pines and other groundcover shrubs and trees, wiregrass modifies fire behavior locally, potentially influencing ecosystem dynamics at larger scales. These results have strong implications for southeastern pine savannas and more broadly where grass-dominated vegetation influences fire regimes. C1 [Fill, Jennifer M.; Mousseau, Timothy A.] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. [Fill, Jennifer M.] Univ Stellenbosch, Dept Bot & Zool, Ctr Invas Biol, ZA-7602 Matieland, South Africa. [Moule, Brett M.] Nat Resource Div III Corps & Ft Hood, Directorate Publ Works, Ft Hood, TX USA. [Varner, J. Morgan] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Varner, J. Morgan] USDA Forest Serv, Pacific Wildland Fire Sci Lab, 400 N 34th St,Suite 201, Seattle, WA 98103 USA. RP Fill, JM (reprint author), Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA.; Fill, JM (reprint author), Univ Stellenbosch, Dept Bot & Zool, Ctr Invas Biol, ZA-7602 Matieland, South Africa. EM jenna999@gmail.com OI Mousseau, Timothy/0000-0002-2235-4868 FU South Carolina Department of Natural Resources; South Carolina Forestry Commission FX Michael Small and Barry Kesler (South Carolina Department of Natural Resources) and Andy Johnson (South Carolina Forestry Commission) provided generous field assistance. Funding was provided by our respective institutions. NR 71 TC 3 Z9 3 U1 4 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD MAR PY 2016 VL 217 IS 3 BP 331 EP 342 DI 10.1007/s11258-016-0574-0 PG 12 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA DM3UJ UT WOS:000376271900009 ER PT J AU Freeman, EA Moisen, GG Coulston, JW Wilson, BT AF Freeman, Elizabeth A. Moisen, Gretchen G. Coulston, John W. Wilson, Barry T. TI Random forests and stochastic gradient boosting for predicting tree canopy cover: comparing tuning processes and model performance SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article; Proceedings Paper CT World Congress of the International-Union-of-Forest-Research-Organizations (IUFRO) CY OCT 05-11, 2014 CL Salt Lake, UT SP Int Union Forest Res Org DE tree canopy cover; predictive mapping; classification and regression trees; random forest; stochastic gradient boosting ID REGRESSION TREES; HYPERSPECTRAL IMAGERY; ABOVEGROUND BIOMASS; UNITED-STATES; CLASSIFICATION; DATABASE; FISH AB As part of the development of the 2011 National Land Cover Database (NLCD) tree canopy cover layer, a pilot project was launched to test the use of high-resolution photography coupled with extensive ancillary data to map the distribution of tree canopy cover over four study regions in the conterminous US. Two stochastic modeling techniques, random forests (RF) and stochastic gradient boosting (SGB), are compared. The objectives of this study were first to explore the sensitivity of RF and SGB to choices in tuning parameters and, second, to compare the performance of the two final models by assessing the importance of, and interaction between, predictor variables, the global accuracy metrics derived from an independent test set, as well as the visual quality of the resultant maps of tree canopy cover. The predictive accuracy of RF and SGB was remarkably similar on all four of our pilot regions. In all four study regions, the independent test set mean squared error (MSE) was identical to three decimal places, with the largest difference in Kansas where RF gave an MSE of 0.0113 and SGB gave an MSE of 0.0117. With correlated predictor variables, SGB had a tendency to concentrate variable importance in fewer variables, whereas RF tended to spread importance among more variables. RF is simpler to implement than SGB, as RF has fewer parameters needing tuning and also was less sensitive to these parameters. As stochastic techniques, both RF and SGB introduce a new component of uncertainty: repeated model runs will potentially result in different final predictions. We demonstrate how RF allows the production of a spatially explicit map of this stochastic uncertainty of the final model. C1 [Freeman, Elizabeth A.; Moisen, Gretchen G.] USDA Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. [Coulston, John W.] USDA Forest Serv, Southern Res Stn, 1710 Res Ctr Dr, Blacksburg, VA 24060 USA. [Wilson, Barry T.] USDA Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. RP Freeman, EA (reprint author), USDA Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. EM eafreeman@fs.fed.us NR 53 TC 3 Z9 3 U1 5 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAR PY 2016 VL 46 IS 3 BP 323 EP 339 DI 10.1139/cjfr-2014-0562 PG 17 WC Forestry SC Forestry GA DL9ER UT WOS:000375944900005 ER PT J AU Pendergast, TH Hanlon, SM Long, ZM Royo, AA Carson, WP AF Pendergast, Thomas H. Hanlon, Shane M. Long, Zachary M. Royo, Alejandro A. Carson, Walter P. TI The legacy of deer overabundance: long-term delays in herbaceous understory recovery SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article; Proceedings Paper CT World Congress of the International-Union-of-Forest-Research-Organizations (IUFRO) CY OCT 05-11, 2014 CL Salt Lake, UT SP Int Union Forest Res Org DE white-tailed deer; Odocoileus virginianus; herbivory; understory; forest recovery ID WHITE-TAILED DEER; ALLEGHENY NATIONAL FOREST; NORTHERN HARDWOOD FOREST; EASTERN DECIDUOUS FOREST; PLANT-COMMUNITIES; VEGETATION RECOVERY; DENSE UNDERSTORY; SUCCESSION; LANDSCAPE; DIVERSITY AB Decades of white-tailed deer (Odocoileus virginianus (Zimmermann, 1780)) overpopulation have dramatically homogenized forests across much of the eastern United States, creating depauperate forest understory communities. The rate at which these communities recover once deer browsing has been reduced remains an open question. We evaluate overbrowsing legacy effects by examining how forest herbaceous layers respond in terms of biodiversity, density, and community composition over 11 years using exclosures and control plots within a mature beech-maple forest. Although little recovery occurred in the first 5 years, total density and preferred browse density rebounded substantially during the final years of the study. Although community composition began to diverge between exclosure and control plots after 5 years, diversity failed to recover even after 11 years of excluding browsers. Our findings show that vulnerable species can increase after excluding browsers but only if those species were initially present. Biodiversity recovery may be extremely slow because preferred browse species have been nearly extirpated from many forests and thus are unable to recruit into refugia. We empirically demonstrate the extent of the ghost of herbivory past or legacy effect of browsing, i.e., the substantial time delay between herbivore abatement and community response after decades of high deer densities. C1 [Pendergast, Thomas H.; Hanlon, Shane M.; Carson, Walter P.] Univ Pittsburgh, Dept Biol Sci, A234 Langley Hall,4249 Fifth Ave, Pittsburgh, PA 15260 USA. [Long, Zachary M.] Univ N Carolina, Dept Biol & Marine Biol, Friday Hall 2015,601 South Coll Rd, Wilmington, NC 28403 USA. [Royo, Alejandro A.] USDA Forest Serv, Forestry Sci Lab, No Res Stn, POB 267, Irvine, PA 16329 USA. [Pendergast, Thomas H.] Univ Georgia, Dept Crop & Soil Sci, 3111 Plant Sci Bldg, Athens, GA 30602 USA. RP Pendergast, TH (reprint author), Univ Pittsburgh, Dept Biol Sci, A234 Langley Hall,4249 Fifth Ave, Pittsburgh, PA 15260 USA.; Pendergast, TH (reprint author), Univ Georgia, Dept Crop & Soil Sci, 3111 Plant Sci Bldg, Athens, GA 30602 USA. EM thpiv@uga.edu NR 53 TC 1 Z9 1 U1 17 U2 23 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAR PY 2016 VL 46 IS 3 BP 362 EP 369 DI 10.1139/cjfr-2015-0280 PG 8 WC Forestry SC Forestry GA DL9ER UT WOS:000375944900008 ER PT J AU Williams, JP Hanavan, RP Rock, BN Minocha, SC Linder, E AF Williams, Justin P. Hanavan, Ryan P. Rock, Barrett N. Minocha, Subhash C. Linder, Ernst TI Influence of hemlock woolly adelgid infestation on the physiological and reflectance characteristics of eastern hemlock SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article; Proceedings Paper CT World Congress of the International-Union-of-Forest-Research-Organizations (IUFRO) CY OCT 05-11, 2014 CL Salt Lake, UT SP Int Union Forest Res Org DE Adelges tsugae; hemlock woolly adelgid; remote sensing; vegetation indices; fluorescence; chlorophyll ID HEMIPTERA ADELGIDAE; ELONGATE HEMLOCK; FOLIAR CHEMISTRY; TSUGA-CANADENSIS; NEW-YORK; CHLOROPHYLL; DECLINE; CAROTENOIDS; RESPONSES; STRESS AB The hemlock woolly adelgid (HWA) (Adelges tsugae Annand) is an invasive insect in the eastern United States. Since its initial detection in Richmond, Virginia, in 1951, HWA has spread to half of the eastern hemlock (Tsuga canadensis (L.) Carr.) natural range. Detection of early infestation symptoms via remote sensing requires the knowledge of the changes in reflectance resulting from physiological changes in the host as inflicted by the insect and the selection of equipment with the appropriate sensor characteristics. Laboratory-based reflectance measurements of infested and non-infested hemlock foliage collected from four sites in southern New Hampshire and Maine occurred biweekly over 6 months in 2012 and weekly over 5 weeks in 2013. Vegetation indices (red edge inflection point (REIP), normalized difference vegetation index (NDVI), moisture stress index (MSI), and near infrared (NIR) 3/1 ratio) were associated with concurrent chlorophyll and moisture content data. Infested first-year foliage contained greater concentrations of chlorophyll and moisture, resulting in reduced visible spectral reflectance, greater REIP and NDVI values, and lower MSI and NIR 3/1 values than non-infested foliage. Furthermore, fluorescence measurements indicated greater photosystem function during the early stages of infestation, suggesting a possible compensatory response by hemlock to infestation. Significant differences in reflectance between infested and non-infested foliage were observed in late June and July in the weeks immediately following HWA settlement on new growth. Implementing these observations during remote sensing mission planning may increase the likelihood of detecting early HWA infestation symptoms at landscape scales. C1 [Williams, Justin P.] Univ New Hampshire, Dept Nat Resources & Environm, James Hall, Durham, NH 03824 USA. [Hanavan, Ryan P.] US Forest Serv, Durham, NH 03824 USA. [Rock, Barrett N.] Univ New Hampshire, Earth Syst Res Ctr, Morse Hall, Durham, NH 03824 USA. [Minocha, Subhash C.] Univ New Hampshire, Dept Biol Sci, Rudman Hall, Durham, NH 03824 USA. [Linder, Ernst] Univ New Hampshire, Dept Math & Stat, Kingsbury Hall, Durham, NH 03284 USA. [Williams, Justin P.] US Forest Serv, Southern Res Stn, Mississippi State, MS 39762 USA. RP Williams, JP (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, James Hall, Durham, NH 03824 USA.; Williams, JP (reprint author), US Forest Serv, Southern Res Stn, Mississippi State, MS 39762 USA. EM justinwilliams@fs.fed.us NR 40 TC 2 Z9 2 U1 9 U2 13 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAR PY 2016 VL 46 IS 3 BP 410 EP 426 DI 10.1139/cjfr-2015-0328 PG 17 WC Forestry SC Forestry GA DL9ER UT WOS:000375944900013 ER PT J AU Shwiff, SA Sweeney, SJ Elser, JL Miller, RS Farnsworth, ML Nol, P Shwiff, SS Anderson, AM AF Shwiff, Stephanie A. Sweeney, Steven J. Elser, Julie L. Miller, Ryan S. Farnsworth, Matthew L. Nol, Pauline Shwiff, Steven S. Anderson, Aaron M. TI A benefit-cost analysis decision framework for mitigation of disease transmission at the wildlife-livestock interface SO HUMAN-WILDLIFE INTERACTIONS LA English DT Article DE benefit-cost analysis; disease management; economics; livestock; wildlife ID MOUTH-DISEASE; UNITED-STATES; CONSERVATION; EMERGENCE; MANAGEMENT; PROJECTS; SUCCESS; RABIES AB The economics of managing disease transmission at the wildlife-livestock interface have received heightened attention as agricultural and natural resource agencies struggle to tackle growing risks to animal health. In the fiscal landscape of increased scrutiny and shrinking budgets, resource managers seek to maximize the benefits and minimize the costs of disease mitigation efforts. To address this issue, a benefit-cost analysis decision framework was developed to help users make informed choices about whether and how to target disease management efforts in wildlife and livestock populations. Within the context of this framework, we examined the conclusions of a bene t-cost analysis conducted for vampire bat (Desmodus rotundus) rabies control in Mexico. The bene t-cost analysis decision framework provides a method that can be used to identify, assemble, and measure the components vital to the biological and economic efficiencies of animal disease mitigation efforts. The framework can be applied to commercially-raised and free-ranging species at various levels of management - from detailed intervention strategies to broad programmatic actions. The ability of benefit-cost analysis to illustrate the benefits of disease management projects per dollar spent allows for the determination of economic efficiency of alternative management actions. We believe this framework will be useful to the broader natural resource management community to maximize returns on financial and other resources invested in wildlife and livestock disease management programs. C1 [Shwiff, Stephanie A.; Elser, Julie L.; Anderson, Aaron M.] Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Sweeney, Steven J.; Miller, Ryan S.; Farnsworth, Matthew L.] Vet Serv, Ctr Epidemiol & Anim Hlth, USDA, APHIS, 2150 Ctr Ave, Ft Collins, CO 80526 USA. [Nol, Pauline] Vet Serv, Wildlife Livestock Dis Invest Team, USDA, APHIS, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Shwiff, Steven S.] Texas A&M Univ Commerce, 2200 Campbell St, Commerce, TX 75428 USA. RP Shwiff, SA (reprint author), Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Stephanie.A.Shwiff@aphis.usda.gov OI Miller, Ryan/0000-0003-3892-0251 NR 36 TC 0 Z9 0 U1 2 U2 3 PU JACK H BERRYMAN INST PI LOGAN PA UTAH STATE UNIV, DEPT WILDLAND RESOURCES, LOGAN, UTAH 84322-5230 USA SN 1934-4392 EI 1936-8046 J9 HUM-WILDL INTERACT JI Hum.-Wildl. Interact. PD SPR PY 2016 VL 10 IS 1 BP 91 EP 102 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DM1GE UT WOS:000376092600012 ER PT J AU Camerota, M Mazza, G Carta, LK Paoli, F Torrini, G Benvenuti, C Carletti, B Francardi, V Roversi, PF AF Camerota, Manuela Mazza, Giuseppe Carta, Lynn K. Paoli, Francesco Torrini, Giulia Benvenuti, Claudia Carletti, Beatrice Francardi, Valeria Roversi, Pio Federico TI Occurrence of Panagrellus (Rhabditida: Panagrolaimidae) Nematodes in a Morphologically Aberrant Adult Specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) SO JOURNAL OF NEMATOLOGY LA English DT Article DE insect thorax defect; invasive species; nematode phoresy; physiological ecology; saprophagous nematode; sour paste nematode ID RIBOSOMAL DNA; CURCULIONIDAE; WEEVIL; SYSTEM; N. AB An aberrant specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) also known as red palm weevil (RPW), the most economically important insect pest of palms in the world, was found among a batch of conspecifics reared for research purposes. A morphological analysis of this weevil revealed the presence of nematodes associated with a structured cuticle defect of the thorax. These nematodes were not able to be cultured, but were characterized by molecular analysis using 28S and 18S ribosomal DNA and shown to belong to the family Panagrolaimidae (Rhabditida), within a clade of Panagrellus. While most nematodes in the insect were juveniles, a single male adult was partially characterized by light microscopy. Morphometrics showed similarities to a species described from Germany. Excluding the entomopathogenic nematodes (EPN), only five other genera of entomophilic or saprophytic rhabditid nematodes are associated with this weevil. This is the first report of panagrolaimid nematodes associated with this invasive pest. Possible mechanisms of nematode-insect association are discussed. C1 [Camerota, Manuela; Mazza, Giuseppe; Paoli, Francesco; Torrini, Giulia; Benvenuti, Claudia; Carletti, Beatrice; Francardi, Valeria; Roversi, Pio Federico] Council Agr Res & Econ, Agrobiol & Pedol Res Ctr CRA ABP, Via Lanciola 12-A, I-50125 Florence, Italy. [Carta, Lynn K.] ARS, Nematol Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Bldg 010A,Room 110, Beltsville, MD 20705 USA. RP Carta, LK (reprint author), ARS, Nematol Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Bldg 010A,Room 110, Beltsville, MD 20705 USA. EM lynn.carta@ars.usda.gov OI Mazza, Giuseppe/0000-0002-6644-1429 NR 25 TC 0 Z9 0 U1 5 U2 6 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2016 VL 48 IS 1 BP 1 EP 6 PG 6 WC Zoology SC Zoology GA DM1PA UT WOS:000376117100001 PM 27168645 ER PT J AU Thies, JA Ariss, JJ Kousik, CS Hassell, RL Levi, A AF Thies, Judy A. Ariss, Jennifer J. Kousik, Chandrasekar S. Hassell, Richard L. Levi, Amnon TI Resistance to Southern Root-knot Nematode (Meloidogyne incognita) in Wild Watermelon (Citrullus lanatus var. citroides) SO JOURNAL OF NEMATOLOGY LA English DT Article DE Citrullus lanatus var. citroides; Citrullus lanatus var. lanatus; Meloidogyne incognita; plant introduction; resistance; southern root-knot nematode; wild watermelon ID GRAFTED WATERMELON; FUSARIUM-WILT; GERMPLASM; ROOTSTOCKS; YIELD AB Southern root-knot nematode (RKN, Meloidogyne incognita) is a serious pest of cultivated watermelon (Citrullus lanatus var. lanatus) in southern regions of the United States and no resistance is known to exist in commercial watermelon cultivars. Wild watermelon relatives (Citrullus lanatus var. citroides) have been shown in greenhouse studies to possess varying degrees of resistance to RKN species. Experiments were conducted over 2 yr to assess resistance of southern RKN in C. lanatus var. citroides accessions from the U.S. Watermelon Plant Introduction Collection in an artificially infested field site at the U.S. Vegetable Laboratory in Charleston, SC. In the first study (2006), 19 accessions of C. lanatus var. citroides were compared with reference entries of Citrullus colocynthis and C. lanatus var. lanatus. Of the wild watermelon accessions, two entries exhibited significantly less galling than all other entries. Five of the best performing C. lanatus var. citroides accessions were evaluated with and without nematicide at the same field site in 2007. Citrullus lanatus var. citroides accessions performed better than C. lanatus var. lanatus and C. colocynthis. Overall, most entries of C. lanatus var. citroides performed similarly with and without nematicide treatment in regard to root galling, visible egg masses, vine vigor, and root mass. In both years of field evaluations, most C. lanatus var. citroides accessions showed lesser degrees of nematode reproduction and higher vigor and root mass than C. colocynthis and C. lanatus var. lanatus. The results of these two field evaluations suggest that wild watermelon populations may be useful sources of resistance to southern RKN. C1 [Thies, Judy A.; Ariss, Jennifer J.; Kousik, Chandrasekar S.; Levi, Amnon] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Hassell, Richard L.] Clemson Univ, Coastal Res & Educ Ctr, Charleston, SC 29414 USA. RP Thies, JA (reprint author), ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. EM judy.thies@comcast.net NR 21 TC 0 Z9 0 U1 5 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2016 VL 48 IS 1 BP 14 EP 19 PG 6 WC Zoology SC Zoology GA DM1PA UT WOS:000376117100004 PM 27168648 ER PT J AU Handoo, ZA Carta, LK Skantar, AM Subbotin, SA Fraedrich, SW AF Handoo, Zafar A. Carta, Lynn K. Skantar, Andrea M. Subbotin, Sergei A. Fraedrich, Stephen W. TI Molecular and Morphological Characterization of Xiphinema chambersi Population from Live Oak in Jekyll Island, Georgia, with Comments on Morphometric Variations SO JOURNAL OF NEMATOLOGY LA English DT Article DE 28S rRNA; live oak; morphology; morphometrics; phylogeny; redescription; taxonomy; tree; Xiphinema chambersi ID AMERICANUM-GROUP; NEMATODA; LONGIDORIDAE; DORYLAIMIDA; SEQUENCES; DNA AB A population of Xiphinema chambersi from the root zone around live oak (Quercus virginiana Mill.) trees on Jekyll Island, GA, is described using both morphological and molecular tools and compared with descriptions of type specimens. Initially, because of a few morphological differences, this nematode was thought to represent an undescribed species. However, on further examination, the morphometrics of the nematodes from live oak tend to agree with most of the morphometrics in the original description and redescription of X. chambersi except for few minor differences in V% relative to body length, slightly shorter stylet length, different c value, and the number of caudal pores. We consider these differences to be part of the normal variation within this species and accordingly image this new population of X. chambersi and redescribe the species. The new population is characterized by having females with a body length of 2.1 to 2.5 mm; lip region slightly rounded and set off from head; total stylet length 170 to 193 mm; vulva at 20.4% to 21.8% of body length; a monodelphic, posterior reproductive system; elongate, conoid tail with a blunt terminus and four pairs of caudal pores, of which two pairs are subdorsal and two subventral. Sequence data from the D2-D3 region of the 28S rRNA molecule subjected to GenBank sequence comparison using BLAST showed that the sequence had 96% and 99% similarity with X. chambersi from Alabama and Florida, respectively. Phylogenetic relationships of X. chambersi with other xiphinematids based on analysis of this DNA fragment are presented. This finding represents a new location of X. chambersi in Georgia on live oak for this species. C1 [Handoo, Zafar A.; Carta, Lynn K.; Skantar, Andrea M.] ARS, Nematol Lab, USDA, Bldg 010A,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Subbotin, Sergei A.] Calif Dept Food & Agr, Plant Pest Diagnost Ctr, 3294 Meadowview Rd, Sacramento, CA 95832 USA. [Fraedrich, Stephen W.] US Forest Serv, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. RP Handoo, ZA (reprint author), ARS, Nematol Lab, USDA, Bldg 010A,BARC West,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM zafar.handoo@ars.usda.gov NR 28 TC 1 Z9 1 U1 4 U2 4 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2016 VL 48 IS 1 BP 20 EP 27 PG 8 WC Zoology SC Zoology GA DM1PA UT WOS:000376117100005 PM 27168649 ER PT J AU Carta, LK Li, SG Skantar, AM Newcombe, G AF Carta, Lynn K. Li, Shiguang Skantar, Andrea M. Newcombe, George TI Morphological and Molecular Characterization of Two Aphelenchoides Endophytic in Poplar Leaves SO JOURNAL OF NEMATOLOGY LA English DT Article DE nematode ecology; phylogeny; ribosomal DNA; systematics; taxonomy ID NEMATODA APHELENCHOIDIDAE; TYLENCHIDA; EVOLUTION; TREES; GENUS AB During a long-term, large network study of the ecology of plant endophytes in native habitats, various nematodes have been found. Two poplar species, Populus angustifolia (narrowleaf cottonwood) and Populus trichocarpa (black cottonwood), are important ecological and genomic models now used in ongoing plant-pathogen-endophyte interaction studies. In this study, two different aphelenchid nematodes within surface-sterilized healthy leaves of these two Populus spp. in northwestern North America were discovered. Nematodes were identified and characterized microscopically and molecularly with 28S ribosomal RNA (rRNA) and 18S rRNA molecular markers. From P. angustifolia, Aphelenchoides saprophilus was inferred to be closest to another population of A. saprophilus among sequenced taxa in the 18S tree. From P. trichocarpa, Laimaphelenchus heidelbergi had a 28S sequence only 1 bp different from that of a Portuguese population, and 1 bp different from the original Australian type population. The 28S and 18S rRNA trees of Aphelenchoides and Laimaphelenchus species indicated L. heidelbergi failed to cluster with three other Laimaphelenchus species, including the type species of the genus. Therefore, we support a conservative definition of the genus Laimaphelenchus, and consider these populations to belong to Aphelenchoides, amended as Aphelenchoides heidelbergi n. comb. This is the first report of these nematode species from within aboveground leaves. The presence of these fungal-feeding nematodes can affect the balance of endophytic fungi, which are important determinants of plant health. C1 [Carta, Lynn K.; Li, Shiguang; Skantar, Andrea M.] ARS, Nematol Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Bldg 010A,Room 110, Beltsville, MD 20705 USA. [Newcombe, George] Univ Idaho, Dept Forest Ecol & Biogeosci, Moscow, ID 83844 USA. RP Carta, LK (reprint author), ARS, Nematol Lab, USDA, Henry A Wallace Beltsville Agr Res Ctr, Bldg 010A,Room 110, Beltsville, MD 20705 USA. EM lynn.carta@ars.usda.gov NR 23 TC 0 Z9 0 U1 3 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2016 VL 48 IS 1 BP 28 EP 33 PG 6 WC Zoology SC Zoology GA DM1PA UT WOS:000376117100006 PM 27168650 ER PT J AU Meyer, SLF Everts, KL Gardener, BM Masler, EP Abdelnabby, HME Skantar, AM AF Meyer, Susan L. F. Everts, Kathryne L. Gardener, Brian McSpadden Masler, Edward P. Abdelnabby, Hazem M. E. Skantar, Andrea M. TI Assessment of DAPG-producing Pseudomonas fluorescens for Management of Meloidogyne incognita and Fusarium oxysporum on Watermelon SO JOURNAL OF NEMATOLOGY LA English DT Article DE beneficial bacteria; biological control; Citrullus lanatus var. lanatus; DAPG; Fusarium oxysporum; Fusarium wilt; management; Meloidogyne incognita; Pseudomonas fluorescens; root-knot nematode; watermelon ID ROOT-KNOT NEMATODE; INDUCED SYSTEMIC RESISTANCE; ANTIBIOTIC PRODUCTION; ARABIDOPSIS-THALIANA; IN-VITRO; 2,4-DIACETYLPHLOROGLUCINOL; BIOCONTROL; RHIZOSPHERE; TOMATO; CHA0 AB Pseudomonas fluorescens isolates Clinto 1R, Wayne 1R, and Wood 1R, which produce the antibiotic 2,4-diacetylphloroglucinol (DAPG), can suppress soilborne diseases and promote plant growth. Consequently, these beneficial bacterial isolates were tested on watermelon plants for suppression of Meloidogyne incognita (root-knot nematode: RKN) and Fusarium oxysporum f. sp. niveum (Fon). In a greenhouse trial, Wayne 1R root dip suppressed numbers of RKN eggs per gram root on 'Charleston Gray' watermelon by 28.9%. However, in studies focused on 'Sugar Baby' watermelon, which is commercially grown in Maryland, a Wayne 1R root dip did not inhibit RKN reproduction or plant death caused by Fon. When all three isolates were applied as seed coats, plant stand in the greenhouse was reduced up to 60% in treatments that included Fon +/- P. fluorescens, and eggs per gram root did not differ among treatments. In a microplot trial with Clinto 1R and Wayne 1R root dips, inoculation with P. fluorescens and/or Fon resulted in shorter vine lengths than treatment with either P. fluorescens isolate plus RKN. Root weights, galling indices, eggs per gram root, and second-stage juvenile (J2) numbers in soil were similar among all RKN-inoculated treatments, and fruit production was not affected by treatment. Plant death was high in all treatments. These studies demonstrated that the tested P. fluorescens isolates resulted in some inhibition of vine growth in the field, and were not effective for enhancing plant vigor or suppressing RKN or Fon on watermelon. C1 [Meyer, Susan L. F.; Masler, Edward P.; Skantar, Andrea M.] ARS, USDA, Nematol Lab, Northeast Area,Henry A Wallace Beltsville Agr Res, Beltsville, MD 20705 USA. [Everts, Kathryne L.] Univ Maryland, College Pk, MD 20742 USA. [Everts, Kathryne L.] Univ Delaware, Lower Eastern Shore Res & Educ Ctr, Salisbury, MD 21801 USA. [Gardener, Brian McSpadden] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Abdelnabby, Hazem M. E.] Benha Univ, Dept Plant Protect, Fac Agr, Qaliubia, Egypt. RP Meyer, SLF (reprint author), ARS, USDA, Nematol Lab, Northeast Area,Henry A Wallace Beltsville Agr Res, Beltsville, MD 20705 USA. EM susan.l.meyer@ars.usda.gov NR 46 TC 1 Z9 1 U1 3 U2 7 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD MAR PY 2016 VL 48 IS 1 BP 43 EP 53 PG 11 WC Zoology SC Zoology GA DM1PA UT WOS:000376117100008 PM 27168652 ER PT J AU Kelleners, TJ Koonce, J Shillito, R Dijkema, J Berli, M Young, MH Frank, JM Massman, WJ AF Kelleners, Thijs J. Koonce, Jeremy Shillito, Rose Dijkema, Jelle Berli, Markus Young, Michael H. Frank, John M. Massman, W. J. TI Numerical Modeling of Coupled Water Flow and Heat Transport in Soil and Snow SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID VAPOR DIFFUSION; ENERGY-BALANCE; THEORETICAL DEVELOPMENT; HYDRAULIC CONDUCTIVITY; POROUS-MEDIA; FROZEN SOIL; EQUATION; EVAPORATION; FLUX AB A one-dimensional vertical numerical model for coupled water flow and heat transport in soil and snow was modified to include all three phases of water: vapor, liquid, and ice. The top boundary condition in the model is driven by incoming precipitation and the surface energy balance. The model was applied to three different terrestrial systems: a warm desert bare lysimeter soil in Boulder City, NV; a cool mixed-grass rangeland soil near Laramie, WY; and a snow-dominated mountainous forest soil about 50 km west of Laramie, WY. Comparison of measured and calculated soil water contents with depth yielded modeling efficiency (ME) values (maximum range: -infinity < ME <= 1) of 0.32 <= ME <= 0.75 for the bare soil, 0.05 <= ME <= 0.30 for the rangeland soil, and 0.06 <= ME <= 0.37 for the forest soil. Results for soil temperature with depth were 0.87 <= ME <= 0.91 for the bare soil, 0.92 <= ME <= 0.94 for the rangeland soil, and 0.85 <= ME <= 0.88 for the forest soil. The model described the mass change in the bare soil lysimeter due to outgoing evaporation with moderate accuracy (ME = 0.41, based on 4 yr of data and using weekly evaporation rates). Snow height for the rangeland soil and the forest soil was captured reasonably well (ME = 0.57 for both sites based on 5 yr of data for each site). The model is physics based, with few empirical parameters, making it applicable to a wide range of terrestrial ecosystems. C1 [Kelleners, Thijs J.] Univ Wyoming, Dept Ecosyst Sci & Management, Laramie, WY 82071 USA. [Koonce, Jeremy; Shillito, Rose; Dijkema, Jelle; Berli, Markus] Desert Res Inst, Div Hydrol Sci, Las Vegas, NV 89119 USA. [Young, Michael H.] Univ Texas Austin, Bur Econ Geol, Austin, TX 78758 USA. [Frank, John M.; Massman, W. J.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Kelleners, TJ (reprint author), Univ Wyoming, Dept Ecosyst Sci & Management, Laramie, WY 82071 USA. EM tkellene@uwyo.edu FU National Science Foundation [EPS-0447416]; University of Wyoming Faculty; National Science Foundation funded Wyoming Center for Environmental Hydrology and Geophysics [EPS-1208909]; University of Wyoming Agricultural Experiment Station FX The SEPHAS large weighing lysimeter study is based on work supported by the National Science Foundation under Grant no. EPS-0447416. The field measurements at the mixed-grass rangeland site were facilitated by an equipment grant from the University of Wyoming Agricultural Experiment Station. Field data collection and modeling work at the mountainous forest site was supported by a University of Wyoming Faculty Grant-In-Aid award and by the National Science Foundation funded Wyoming Center for Environmental Hydrology and Geophysics under Grant no. EPS-1208909. The comments of three anonymous reviewers and the associate editor helped to improve the paper. NR 59 TC 2 Z9 2 U1 12 U2 22 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2016 VL 80 IS 2 BP 247 EP 263 DI 10.2136/sssaj2015.07.0279 PG 17 WC Soil Science SC Agriculture GA DM5OM UT WOS:000376399200001 ER PT J AU Beaudette, DE O'Geen, AT AF Beaudette, D. E. O'Geen, A. T. TI Topographic and Geologic Controls on Soil Variability in California's Sierra Nevada Foothill Region SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID X-RAY-FLUORESCENCE; SPATIAL PREDICTION; REGULARIZED SPLINE; DEPTH FUNCTIONS; LANDSCAPE; CLASSIFICATION; DISAGGREGATION; INTERPOLATION; EVOLUTION; TEXTURE AB We evaluated the feasibility of quantitative soil mapping in two catenas established on different lithologies (metavolcanic and granitic) in the Sierra Foothill Region of California. Indices of landform and microclimate were extracted from a 1-m elevation model. Variation in soil "character" (clay content, pH, color, cation-exchange capacity [CEC], and Fe-o/ Fe-d) was partitioned across variables associated with terrain shape and microclimate, lithologic variability, and sampling depth. The potential for using digital elevation models (DEM)-derived indices of terrain shape to predict spatial patterns in soil properties varied greatly between our two experimental catenas. Terrain shape accounted for 4% (metavolcanic site) to 30% (granitic site) of variance in soil properties, while lithology accounted for 14% (metavolcanic site) to 22% (granitic site) of variance in soil properties. Sample depth accounted for 3% (metavolcanic site) to 12% (granitic site) of variance in soil properties. At the metavolcanic site, variability in lithology contributed more to soil variation than terrain shape, which makes digital soil modeling efforts a challenge in these regions. Up to 66% of the variance in soil properties was explained at the granitic site when considering terrain, lithology, sample depth, and associated interactions of these variables. Variance proportions can provide insight into the relative importance of soil-forming factors and is a useful tool when evaluating the efficacy of digital soil mapping projects. C1 [Beaudette, D. E.] USDA NRCS Soil Survey, 19777 Greenley Rd, Sonora, CA 95370 USA. [O'Geen, A. T.] Univ Calif Davis, Land Air & Water Resources, One Shields Ave, Davis, CA 95616 USA. RP Beaudette, DE (reprint author), USDA NRCS Soil Survey, 19777 Greenley Rd, Sonora, CA 95370 USA. EM debeaudette@ucdavis.edu FU Kearney Foundation of Soil Science; National Science Foundation [1331939] FX We thank Alexandre Swarowsky and Tony Orozco for assistance with soil description and sampling SFREC and SJER catenas. We also thank Jaiyou Deng for his contributions to laboratory work supporting this study, and the staff at SJER and SFREC for their support. We would like to thank Dr. Laura Stupi (Thermo Fischer Scientific) for lending us two portable XRF devices over the summer of 2010. This work was funded by the Kearney Foundation of Soil Science and in part by the National Science Foundation, award #1331939. NR 70 TC 0 Z9 0 U1 9 U2 11 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2016 VL 80 IS 2 BP 341 EP 354 DI 10.2136/sssaj2015.07.0251 PG 14 WC Soil Science SC Agriculture GA DM5OM UT WOS:000376399200009 ER PT J AU Graham, RC Egerton-Warburton, LM Hendrix, PF Shouse, PJ Johnson-Maynard, JL Quideau, SA Sternberg, PD Jobes, JA Breiner, JM AF Graham, Robert C. Egerton-Warburton, Louise M. Hendrix, Paul F. Shouse, Peter J. Johnson-Maynard, Jodi L. Quideau, Sylvie A. Sternberg, Paul D. Jobes, Jack A. Breiner, Joan M. TI Wildfire Effects on Soils of a 55-Year-Old Chaparral and Pine Biosequence SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID SOUTHERN CALIFORNIA; WATER REPELLENCY; 4 DECADES; BASE CATION; WOOD-ASH; FIRE; FOREST; VEGETATION; ECOSYSTEM; CARBON AB A chaparral and pine biosequence established in 1946 was burned in a wildfire in 2002. Thorough studies of the soil environments during the previous two decades established a baseline for comparison of post-fire changes. Aboveground vegetation was reduced to charred stems, and litter layers were combusted to white ash and charred material. Nevertheless, during the first year after the fire, runoff and erosion were minimal because biotically produced macropores maintained high infiltration rates. Loss of cover and plant transpiration made the soil warmer and moister. Combustion of organic matter mineralized constituent cations, increasing base cation concentrations in soil solutions and solid phases within the first few months after the fire. The pH, C, and N also increased in the soil, and the biological environment was extensively altered. Surface-active macrofauna increased, though seasonal patterns of abundance were unchanged. Post-fire fungal communities were distinctly different from pre-fire communities and were more similar to each other than were pre-fire communities. Overall, many of the wildfire impacts appear to be of short duration. Within 6 mo, original species were resprouting or emerging as seedlings, and most soil chemical, physical, and biological properties were either similar to pre-fire conditions or were rapidly returning toward them. C1 [Graham, Robert C.; Sternberg, Paul D.] Univ Calif Riverside, Soil & Water Sci Program, Riverside, CA 92521 USA. [Egerton-Warburton, Louise M.] Chicago Bot Garden, Glencoe, IL 60022 USA. [Hendrix, Paul F.] Univ Georgia, Inst Ecol, Athens, GA 30602 USA. [Shouse, Peter J.; Jobes, Jack A.] USDA ARS, George E Brown Jr Salin Lab, Riverside, CA 92507 USA. [Johnson-Maynard, Jodi L.] Univ Idaho, Soil Sci Div, Moscow, ID 83844 USA. [Quideau, Sylvie A.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada. [Breiner, Joan M.] North Dakota Publ Serv Commiss, Bismarck, ND 58505 USA. RP Graham, RC (reprint author), Univ Calif Riverside, Soil & Water Sci Program, Riverside, CA 92521 USA. EM robert.graham@ucr.edu RI Johnson-Maynard, Jodi/E-9518-2013 FU Agricultural Experiment Station Project [CA-R*-ENS-5889-H]; National Science Foundation; Kearney Foundation of Soil Science; National Commission on Science for Sustainable Forestry FX We are most grateful for the use of the US Forest Service Pacific Southwest facilities at the San Dimas Experimental Forest and for the assistance of Mike Oxford, the on-site manager. This research was supported by Agricultural Experiment Station Project CA-R*-ENS-5889-H and by grants from the National Science Foundation, the Kearney Foundation of Soil Science, and the National Commission on Science for Sustainable Forestry. NR 75 TC 1 Z9 1 U1 12 U2 24 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2016 VL 80 IS 2 BP 376 EP 394 DI 10.2136/sssaj2015.09.0317 PG 19 WC Soil Science SC Agriculture GA DM5OM UT WOS:000376399200012 ER PT J AU Morrow, JG Huggins, DR Carpenter-Boggs, LA Reganold, JP AF Morrow, Jason G. Huggins, David R. Carpenter-Boggs, Lynne A. Reganold, John P. TI Evaluating Measures to Assess Soil Health in Long-Term Agroecosystem Trials SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER; NITROGEN MINERALIZATION; GREAT-PLAINS; CARBON POOLS; MANAGEMENT; QUALITY; LABILE; DYNAMICS; TILLAGE; CROP AB Monitoring soil health is an important component of any land management system that sustains soil resources. As metrics of soil health, we evaluated surface soil organic matter (SOM) properties from five field experiments ranging from 2 to 30 yr old and representing diverse agroecosystems across the inland Pacific Northwest (iPNW). The SOM properties measured included soil organic C (SOC), total N, acid nonhydrolyzable C (NHC), acid nonhydrolyzable N (NHN), acid-hydrolyzable C (HC), acid-hydrolyzable N (HN), microbial biomass C (MBC), microbial biomass N (MBN), carbon mineralization (Cmin), permanganate oxidizable C (POXC), ion exchange membrane N (IEM N), and potential N mineralization (PNM). We further evaluated these SOM metrics and the Haney soil health index (SHindex) relative to the following seven criteria as a framework to judge the effectiveness of soil health tools: (i) evidence based, (ii) sensitive to change, (iii) logistically sensitive, (iv) cost effective, (v) accurate and precise, (vi) performed in situ, and (vii) valued for management decisions. Measures of active SOM were highly variable, particularly 1-d Cmin (CV, 3-53%), IEM N (CV, 9-55%), and SHindex (CV, 4-37%) and subsequently not highly sensitive to management. Permanganate oxidizable C displayed sensitivity to more stabilized SOM, indicated by strong correlations to NHC (r = 0.84) and NHN (r = 0.80), and coupled with IEM N provided complimentary information important to soil health. Using these seven criteria to gauge soil health metrics, POXC scored the highest and should be considered as a component for soil health assessment within the iPNW. C1 [Morrow, Jason G.; Carpenter-Boggs, Lynne A.; Reganold, John P.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Huggins, David R.] Washington State Univ, USDA ARS, Land Management & Water Conserv Unit, Pullman, WA 99164 USA. RP Morrow, JG (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM jason.morrow@wsu.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-68002-30191] FX This material is based on work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2011-68002-30191. We would also like to acknowledge and thank the site leaders of the research sites represented in this study: Jody Johnson-Maynard, Kambitsch Farm, Genesee, ID; Stephen Machado, Columbia Basin Agriculture Research Center, Moro, OR; Hero Gallony, Columbia Basin Agriculture Research Center, Pendleton, OR; Hal Collins, WSU Irrigated Agricultural Research Center, Prosser, WA. The authors would also like to thank two anonymous reviewers for comments that helped improve the manuscript. NR 51 TC 2 Z9 3 U1 20 U2 37 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2016 VL 80 IS 2 BP 450 EP 462 DI 10.2136/sssaj2015.08.0308 PG 13 WC Soil Science SC Agriculture GA DM5OM UT WOS:000376399200018 ER PT J AU Wuest, SB Reardon, CL AF Wuest, Stewart B. Reardon, Catherine L. TI Surface and Root Inputs Produce Different Carbon/Phosphorus Ratios in Soil SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER POOLS; CENTRAL GREAT-PLAINS; ENZYME-ACTIVITIES; CARBON SEQUESTRATION; WHEAT-FALLOW; PHOSPHORUS; DYNAMICS; NITROGEN; TILLAGE; LITTER AB Formation of soil organic C (SOC) is influenced by inputs. We applied organic amendments for five consecutive years at 250 g C m(-2) yr(-1). Seven years after the applications ended, the effects of biosolids and manure on SOC were greater than alfalfa (Medicago sativa L.), wood, wheat (Triticum aestivum L.) residue, sucrose, brassica residue, cotton (Gossypium hirsutum L.), wheat compost, or the unamended check. Soil C increases ranged from 3 to 49% of C applied and had changed little in the previous 28 mo. Plots sown to continuous winter wheat during the period of amendment had 10% more SOC than plots that had been fallow, an effect more than twice as large as most amendments. This suggests that the contribution of roots to SOC was more important than aboveground crop residues. The effect of amendments on SOC was highly correlated to their initial P content. In addition, for a similar available soil P and soil S content, SOC was significantly greater where wheat or perennial grass was grown. Wood caused an increase in the C/N ratio that persisted >3.5 yr but almost disappeared by the seventh year. This study indicated that continuous cropping or the addition of animal waste or municipal biosolids had the greatest impact on the formation of SOC, and this positive effect remained stable for many years after the end of the continuous treatment applications. C1 [Wuest, Stewart B.; Reardon, Catherine L.] USDA ARS, Soil & Water Res Unit, Pendleton, OR 97801 USA. RP Wuest, SB (reprint author), USDA ARS, Soil & Water Res Unit, Pendleton, OR 97801 USA. EM stewart.wuest@ars.usda.gov NR 27 TC 1 Z9 1 U1 5 U2 12 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAR-APR PY 2016 VL 80 IS 2 BP 463 EP 471 DI 10.2136/sssaj2015.09.0334 PG 9 WC Soil Science SC Agriculture GA DM5OM UT WOS:000376399200019 ER PT J AU McKee, GA Soong, JL Calderon, F Borch, T Cotrufo, MF AF McKee, Georgina A. Soong, Jennifer L. Calderon, Francisco Borch, Thomas Cotrufo, M. Francesca TI An integrated spectroscopic and wet chemical approach to investigate grass litter decomposition chemistry SO BIOGEOCHEMISTRY LA English DT Article DE Litter decomposition; Cellulose; Hemicellulose; Lignin; NMR; FTIR; Grass; Big bluestem ID SOIL ORGANIC-MATTER; STATE C-13 NMR; NUCLEAR-MAGNETIC-RESONANCE; ACID-DETERGENT FIBER; FT-IR SPECTROSCOPY; CROSS-POLARIZATION; BIG BLUESTEM; INFRARED-SPECTROSCOPY; DRIFT SPECTROSCOPY; FOREST SOILS AB The chemical transformations that occur during litter decomposition are key processes for soil organic matter formation and terrestrial biogeochemistry; yet we still lack complete understanding of these chemical processes. Thus, we monitored the chemical composition of Andropogon gerardii (big bluestem grass) litter residue over a 36 month decomposition experiment in a prairie ecosystem using: traditional wet chemical fractionation based upon digestibility, solid state C-13 nuclear magnetic resonance (NMR) spectroscopy and Fourier transform infrared (FTIR) spectroscopy. The goals of this study were to (1) determine the chemical changes occurring during A. gerardii litter decomposition, and (2) compare the information obtained from each method to assess agreement. Overall, we observed a 97 % mass loss of the original litter, through a two-stage decomposition process. In the first stage, within 12 months, non-structural, cellulose and hemicellulose fractions not encrusted in lignin were preferentially and rapidly lost, while the acid unhydrolyzable residue (AUR) and microbial components increased. During the second stage, 12-36 months, all wet chemical fraction masses decreased equivalently and slowly with time, and the AUR and the lignin-encrusted cellulose fractions decomposition rates were comparable to each other. Method comparisons revealed that wet chemical fractionation did not accurately follow the initial litter structures, particularly lignin, likely because of chemical transformations and accumulation of microbial biomass. FTIR and NMR were able to determine bulk structural characteristics, and aid in elucidating chemical transformations but lacked the ability to measure absolute quantities of structural groups. As a result, we warn from the sole use of wet chemical methods, and strongly encourage coupling them with spectroscopic methods. Our results overall support the traditional chemical model of selective preservation of lignin, but shows that this is limited to the early stages of decomposition, while lignin is not selectively preserved at subsequent stages. Our study also provides important evidence regarding the impact of chemically different litter structures on decomposition rates and pathways. C1 [McKee, Georgina A.; Borch, Thomas; Cotrufo, M. Francesca] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Soong, Jennifer L.; Cotrufo, M. Francesca] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Calderon, Francisco] USDA, ARS, Akron, CO 80526 USA. [Borch, Thomas] Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA. [Soong, Jennifer L.] Univ Antwerp, Dept Biol, B-2610 Antwerp, Belgium. RP McKee, GA (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM mckee.georgina@gmail.com OI Borch, Thomas/0000-0002-4251-1613 FU Konza Prairie LTER laboratory; EcoCore laboratory; National Science Foundation (NSF)-Division of Environmental Biology [0918482]; NSF; United States Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative [2012-01330]; Cotrufo-Hoppess fund for soil ecology research; Office of Biological and Environmental Research FX We would like to thank the Konza Prairie LTER and EcoCore laboratories for their support and use of their facilities. The work was funded by the National Science Foundation (NSF)-Division of Environmental Biology grant #0918482, the NSF Graduate Research Fellowship Program, the United States Department of Agriculture National Institute of Food and Agriculture, Agriculture and Food Research Initiative postdoctoral fellowship grant # 2012-01330, and the Cotrufo-Hoppess fund for soil ecology research. The wet chemical work was carried out at the EcoCore analytical services facility at Colorado State University (http://ecocore.nrel.colostate.edu/) with the help of Dr Liping Qiu. A portion of the research was performed using EMSL, a DOE Office of Science User Facility sponsored by the Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory. We would like to thank the scientists at EMSL who assisted in running the NMR samples, particularly Dr. Sarah Burton. NR 71 TC 3 Z9 3 U1 9 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD MAR PY 2016 VL 128 IS 1-2 BP 107 EP 123 DI 10.1007/s10533-016-0197-5 PG 17 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DJ9UB UT WOS:000374557300007 ER PT J AU Liu, S Gao, P Zhu, QL Luan, FS Davis, AR Wang, XL AF Liu, Shi Gao, Peng Zhu, Qianglong Luan, Feishi Davis, Angela R. Wang, Xiaolu TI Development of cleaved amplified polymorphic sequence markers and a CAPS-based genetic linkage map in watermelon (Citrullus lanatus [Thunb.] Matsum. and Nakai) constructed using whole-genome re-sequencing data SO BREEDING SCIENCE LA English DT Article DE SNP; CAPS; watermelon; re-sequencing data; linkage map; Perl; Citrullus ID CUCUMIS-MELO L.; SINGLE NUCLEOTIDE POLYMORPHISMS; RECOMBINANT INBRED LINES; DRAFT GENOME; FLESH COLOR; INDEL MARKERS; CANARY YELLOW; PCR MARKERS; DIVERSITY; RESISTANCE AB Cleaved amplified polymorphic sequence (CAPS) markers are useful tools for detecting single nucleotide polymorphisms (SNPs). This study detected and converted SNP sites into CAPS markers based on high throughput re-sequencing data in watermelon, for linkage map construction and quantitative trait locus (QTL) analysis. Two inbred lines, Cream of Saskatchewan (COS) and LSW-177 had been re-sequenced and analyzed by Perl self-compiled script for CAPS marker development. 88.7% and 78.5% of the assembled sequences of the two parental materials could map to the reference watermelon genome, respectively. Comparative assembled genome data analysis provided 225,693 and 19,268 SNPs and indels between the two materials. 532 pairs of CAPS markers were designed with 16 restriction enzymes, among which 271 pairs of primers gave distinct bands of the expected length and polymorphic bands, via PCR and enzyme digestion, with a polymorphic rate of 50.94%. Using the new CAPS markers, an initial CAPS-based genetic linkage map was constructed with the F-2 population, spanning 1836.51 cM with 11 linkage groups and 301 markers. 12 QTLs were detected related to fruit flesh color, length, width, shape index, and brix content. These newly CAPS markers will be a valuable resource for breeding programs and genetic studies of watermelon. C1 [Liu, Shi; Gao, Peng; Zhu, Qianglong; Luan, Feishi; Wang, Xiaolu] Northeast Agr Univ, Hort Coll, 59 Mucai St, Harbin 150030, Heilongjiang Pr, Peoples R China. [Liu, Shi; Gao, Peng; Zhu, Qianglong; Luan, Feishi; Wang, Xiaolu] Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, 59 Mucai St, Harbin 150030, Heilongjiang Pr, Peoples R China. [Davis, Angela R.] ARS, South Cent Agr Res Lab, USDA, 9241 Mace Blvd, Davis, CA 95618 USA. RP Luan, FS (reprint author), Northeast Agr Univ, Hort Coll, 59 Mucai St, Harbin 150030, Heilongjiang Pr, Peoples R China. EM luanfeishi@neau.edu.cn FU National Nature Science Foundation of China [31272186]; China Agriculture Research System [CARS-26-02] FX This research was supported by the National Nature Science Foundation of China (31272186). This work was also supported by the China Agriculture Research System (CARS-26-02). NR 72 TC 0 Z9 0 U1 9 U2 13 PU JAPANESE SOC BREEDING PI TOKYO PA UNIV TOKYO, GRADUATE SCHOOL AGRICULTURAL LIFE SCIENCES, BUNKYO-KU, TOKYO, 113-8657, JAPAN SN 1344-7610 EI 1347-3735 J9 BREEDING SCI JI Breed. Sci. PD MAR PY 2016 VL 66 IS 2 BP 244 EP 259 DI 10.1270/jsbbs.66.244 PG 16 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DL4SH UT WOS:000375627000010 PM 27162496 ER PT J AU Tait, RG Cushman, RA McNeel, AK Casas, E Smith, TPL Freetly, HC Bennett, GL AF Tait, R. G., Jr. Cushman, R. A. McNeel, A. K. Casas, E. Smith, T. P. L. Freetly, H. C. Bennett, G. L. TI Estimates of epistatic and pleiotropic effects of casein alpha s1 (CSN1S1) and thyroglobulin (TG) genetic markers on beef heifer performance traits enhanced by selection SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE body weight; CSN1S1; fertility; marker association; reproduction; TG ID CARCASS QUALITY TRAITS; PREWEANING PERFORMANCE; RESIDUAL VARIANCE; BIRTH-WEIGHT; MU-CALPAIN; CATTLE; POLYMORPHISM; OVARIAN; TENDERNESS; FERTILITY AB Genetic marker effects and type of inheritance are estimated with poor precision when minor marker allele frequencies are low. A stable composite population (MARC II) was subjected to marker assisted selection for 2 yr to equalize CSN1S1 and TG genetic marker frequencies to evaluate the epistatic and pleiotropic effects of these markers on BW, reproduction, and first calf performance traits in replacement beef females (n = 171) managed under 2 postweaning development protocols. Traits evaluated on the heifers were birth BW, weaning BW, 11-mo BW, 12-mo BW, 13-mo BW, first breeding season pregnancy evaluation BW, first calving season BW, 11-mo puberty, 12-mo puberty, 13-mo puberty, first breeding season pregnancy, and first calf weaning rate. Additionally, heifer's first calf performance traits of ordinal calving date, first calf birth BW, and first calf weaning BW (with and without age adjustment) were analyzed. Selection to increase minor allele frequencies and balanced sampling across genotype classes enhanced the ability to detect all genetic effects except dominance x dominance epistasis. The CSN1S1 x TG genotype effect was significant (P < 0.05) for 11-mo BW and 12-mo BW and tended to be significant (P = 0.08) for 13-mo BW. Consistently, for all 3 traits, the most significant effect among epistatic CSN1S1 x TG genotype effects was the CSN1S1 additive effect, with the G allele decreasing BW. There were no associations between CSN1S1 x TG genotype and fertility related traits (P = 0.46) in this study. Additionally, there were no CSN1S1 x TG genotype associations with first progeny performance traits (P = 0.14). The large effect of the CSN1S1 additive x TG additive interaction on first calf weaning BW was imprecisely estimated, which may warrant further investigation. C1 [Tait, R. G., Jr.; Cushman, R. A.; McNeel, A. K.; Casas, E.; Smith, T. P. L.; Freetly, H. C.; Bennett, G. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [McNeel, A. K.] Zoetis Inc, 333 Portage St,300-210SE, Kalamazoo, MI 49007 USA. [Casas, E.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Bennett, GL (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM gary.bennett@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2016 VL 94 IS 3 BP 920 EP 926 DI 10.2527/jas2015-9860 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RI UT WOS:000374690800004 PM 27065254 ER PT J AU Veum, TL Raboy, V AF Veum, T. L. Raboy, V. TI Hulled and hull-less barley grains with the genetic trait for low-phytic acid increased the apparent total-tract digestibility of phosphorus and calcium in diets for young swine SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE apparent calcium and phosphorus digestibility; apparent energy and dry matter digestibility; bone breaking strength; growth performance; phosphorus sustainability; serum phosphatase enzymes ID BETA-GLUCANASE SUPPLEMENTATION; IMPROVES NUTRIENT UTILIZATION; SOYBEAN MEAL DIETS; GROWTH-PERFORMANCE; LOW-PHYTATE; AVAILABLE PHOSPHORUS; GROWING-PIGS; FINISHING PIGS; NONSTARCH POLYSACCHARIDES; INORGANIC PHOSPHORUS AB A 35-d experiment was conducted using 63 crossbred pigs (35 barrows and 28 gilts) with an initial average BW of 7.0 kg and age of 28 d to evaluate the efficacy of the low-phytic acid (LPA) genetic trait in hulled or hull-less barley in isocaloric diets. Hulled barleys were the normal barley (NB) cultivar Harrington and the near-isogenic LPA mutant 955 (M955) with P availabilities of 36 and 95%, respectively. Hull-less lines were produced by crossing NB and the LPA mutant 422 line with a hull-less line, producing hull-less NB (HNB) and hull-less mutant 422 (HM422) with P availabilities of 41 and 66%, respectively. Pigs were in individual metabolism cages or pens for Phase 1 (d 0 to 14) and Phase 2 (d 14 to 35). Diets defined as NB, HNB, HM422, or M955 with no added inorganic P (iP) had available P (aP) concentrations of 0.27, 0.28, 0.35, and 0.40% for Phase 1 and 0.15, 0.17, 0.23, and 0.31% for Phase 2, respectively. Only diet M955 was adequate in aP. Therefore, iP was added to the P-deficient diets to make diets NB + iP, HNB + iP, and HM422 + iP with aP equal to that in diet M955. Overall (d 0 to 35), ADG and G:F were greater (P < 0.01) for pigs fed diet M955 or the diets with added iP than for pigs fed the NB diet. Serum tartrate-resistant acid phosphatase activity on d 34 was greater (P < 0.01) for pigs fed the NB or HNB diets than for pigs fed the other diets. Bone breaking strength and P absorption (g/d) were greater (P < 0.01) for pigs fed diet M955 or the diets with iP than for pigs fed the NB or HNB diets. Pigs fed diet M955 absorbed greater (P < 0.01) percentages of P and Ca and had less (P < 0.01) fecal excretion of P (g/d and %) and Ca (%) than pigs fed the other diets. In conclusion, the LPA genetic trait was effective in hulled and hull-less barley in isocaloric diets fed to young pigs. Pigs fed the diet with LPA M955 consumed 31% less P and excreted 78% less fecal P and 30% less fecal Ca than pigs fed the diet with NB + iP that was equal to diet M955 in aP. Therefore, LPA barley, especially M955 with 95% aP, will reduce the use of iP in swine diets, reduce P pollution from swine manure, and support the goal of achieving global P sustainability. C1 [Veum, T. L.] Univ Missouri, Div Anim Sci, Agr Expt Stn, Columbia, MO 65211 USA. [Raboy, V.] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. RP Veum, TL (reprint author), Univ Missouri, Div Anim Sci, Agr Expt Stn, Columbia, MO 65211 USA. EM veumt@missouri.edu FU Missouri Agric. Exp. Sta.; USDA-ARS Small Grains and Potato Germplasm Research Unit, Aberdeen, ID FX Supported by the Missouri Agric. Exp. Sta. and the USDA-ARS Small Grains and Potato Germplasm Research Unit, Aberdeen, ID. A. Bauer, G. Beerman, D. Bollinger, H. Hellman, C. Huntington, C. Kinneson, E. Kobashigawa, and A. Rieke are thanked for technical assistance. NR 84 TC 0 Z9 0 U1 5 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAR PY 2016 VL 94 IS 3 BP 1000 EP 1011 DI 10.2527/jas2015-9994 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK1RI UT WOS:000374690800012 PM 27065262 ER PT J AU Altenbach, SB Tanaka, CK Whitehand, LC Vensel, WH AF Altenbach, Susan B. Tanaka, Charlene K. Whitehand, Linda C. Vensel, William H. TI Effects of post-anthesis fertilizer on the protein composition of the gluten polymer in a US bread wheat SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Chain terminators; Flour quality; Gluten polymer; Glutenins ID 2-DIMENSIONAL GEL-ELECTROPHORESIS; TANDEM MASS-SPECTROMETRY; BIOCHEMICAL BASIS; FLOUR PROPERTIES; QUALITY; GRAIN; ACCUMULATION; SUBUNITS; FUNCTIONALITY; TEMPERATURE AB Both genetic and environmental factors influence the types and amounts of wheat proteins that link together to form polymers essential for flour quality. To understand how plant growth conditions might influence gluten polymer formation, protein fractions containing small and large polymers were separated from flour from the US wheat Butte 86 grown in the absence or presence of post-anthesis fertilizer. Proteins in the polymer fractions were analyzed by quantitative two-dimensional gel electrophoresis (2-DE). The ratio of high molecular weight glutenin subunits (HMW-GS) to low molecular weight glutenin subunits (LMW-GS) increased in both fractions in response to fertilizer, due in part to small increases in the proportions of individual HMW-GS. There were also changes within the LMW-GS. In particular, omega and alpha chain terminators increased in proportion in both polymer fractions, but changes were more pronounced in the large polymer fractions. Serpins also increased in both polymer fractions. Additionally, the study revealed differences in the proportions of traditional LMW-GS in small and large polymer fractions. LMW-s type proteins were more abundant in the large polymers while LMW-i type proteins were more prevalent in the small polymers, suggesting that these proteins may play different roles in the gluten polymer. Published by Elsevier Ltd. C1 [Altenbach, Susan B.; Tanaka, Charlene K.; Whitehand, Linda C.; Vensel, William H.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Altenbach, SB (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM susan.altenbach@ars.usda.gov FU USDA Agricultural Research Service CRIS Project [5325-43000-028-00D] FX Research was funded by USDA Agricultural Research Service CRIS Project 5325-43000-028-00D. USDA is an equal opportunity provider and employer. Mention of a specific product name by the United States Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 23 TC 0 Z9 0 U1 3 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD MAR PY 2016 VL 68 BP 66 EP 73 DI 10.1016/j.jcs.2015.12.002 PG 8 WC Food Science & Technology SC Food Science & Technology GA DJ6ZN UT WOS:000374361800010 ER PT J AU Simsek, S Ohm, JB Cariou, V Mergoum, M AF Simsek, Senay Ohm, Jae-Bom Cariou, Vincent Mergoum, Mohamed TI Effect of flour polymeric proteins on dough thermal properties and breadmaking characteristics for hard red spring wheat genotypes SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Polymeric proteins; Dough thermal property; Hard red spring wheat ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SIZE DISTRIBUTION; RHEOLOGICAL BEHAVIOR; GLUTENIN; TEMPERATURE; SONICATION; QUALITY; DEPOLYMERIZATION; PARAMETERS; QUANTITY AB The aim of this research was to investigate the effect of variation of flour polymeric proteins on dough thermal properties for hard red spring (HRS) wheat genotypes grown in the U.S. state of North Dalcota. Flour polymeric proteins were analyzed by size exclusion HPLC for sodium dodecyl sulfate (SDS) buffer extractable and unextractable fractions. Mixolab was employed to measure dough thermal property. The Mixolab torque profile was fitted to nonlinear equations, and envelope amplitude value was calculated by treating the torque profile as a wave. The dough tolerance to thermal weakening, as determined from the Mixolab parameters (including the constants of a nonlinear model and envelope torque amplitude values derived from the mixing torque profile during heating from 30 to 70 degrees C), was identified to have significant (P < 0.05) and positive correlations with flour bread loaf volume potential. Variations of high molecular weight polymeric proteins appeared to affect dough tolerance to thermal weakening and bread loaf volume for HRS wheat genotypes, and different associations with breadmaking characteristics were observed according to extractability in SDS buffer. Negative correlations with tolerance to thermal weakening and bread loaf volume were seen for SDS extractable polymeric proteins and positive correlations were observed for unextractable polymeric proteins. Published by Elsevier Ltd. C1 [Simsek, Senay; Cariou, Vincent; Mergoum, Mohamed] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Ohm, Jae-Bom] USDA ARS, Cereal Crops Res Unit, Hard Spring & Durum Wheat Qual Lab, Fargo, ND USA. RP Ohm, JB (reprint author), N Dakota State Univ, NDSU Dept 7640, POB 6050, Fargo, ND 58105 USA. EM jae.ohm@ars.usda.gov FU USDA-ARS CRIS [3660-43440-011-00D] FX We wish to express our gratitude to Chopin (Villeneuve la Garenne, France), Northern Crop Institute, (Fargo, ND), NDSU Hard Spring Wheat Quality Laboratory, and NDSU Hard Spring Wheat Breeding Program for their help in this research. We are also grateful to Stephanie Ness and Jan Ohm for attentive reading and technical help in preparing this manuscript. Part of this work was supported by the USDA-ARS CRIS Project No. 3660-43440-011-00D. NR 24 TC 0 Z9 0 U1 5 U2 5 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD MAR PY 2016 VL 68 BP 164 EP 171 DI 10.1016/j.jcs.2016.01.003 PG 8 WC Food Science & Technology SC Food Science & Technology GA DJ6ZN UT WOS:000374361800023 ER PT J AU Blechl, A Beecher, B Vensel, W Tanaka, C Altenbach, S AF Blechl, Ann Beecher, Brian Vensel, William Tanaka, Charlene Altenbach, Susan TI RNA interference targeting rye secalins alters flour protein composition in a wheat variety carrying a 1BL.1RS translocation SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Transgenic; Triticum aestivum; Down-regulation; Secale cereale ID BREAD WHEAT; STORAGE PROTEINS; TRANSGENIC WHEAT; GRAIN-YIELD; ENDOSPERM; GENE; ELECTROPHORESIS; EXPRESSION; LINES AB Wheat varieties carrying chromosome translocations from rye are part of the international wheat breeding pool despite being associated with defects in dough processing quality. One cause proposed for the quality defects of wheat carrying 1BL1RS translocations is the presence of the secalins encoded by the Sec1 locus on the short arm of rye chromosome 1. In this report, we decrease the levels of these and related wheat proteins by RNA interference (RNAi). A plasmid designed to down-regulate secalin was introduced by biolistics into 'Bobwhite', which carries a 1BL.1RS translocation. Flour proteins of two independent transformants were analyzed in detail by 2-dimensional gel electrophoresis and tandem mass spectrometry. Compared to the parent, the transformants exhibited up to 5.5-fold decreases in the levels of individual secalins, up to 7-fold decreases in the closely related omega gliadins, and lesser decreases in many gamma gliadins, low-molecular-weight (LMW)-glutenins, and farinins. Increased levels were found for nearly all identified alpha gliadins, triticins, and y-type high-molecular-weight (HMW)-glutenins. Flour from the transgenic lines exhibited changes in dough mixing behavior in the 2-g mixograph, including longer development times and greater tolerance to mixing. We conclude that changes in protein composition engineered by this RNAi construct improved dough handling characteristics. Published by Elsevier Ltd. C1 [Blechl, Ann; Vensel, William; Tanaka, Charlene; Altenbach, Susan] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Beecher, Brian] USDA, GIPSA, 10383 North Ambassador Dr, Kansas City, MO 64153 USA. RP Blechl, A (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM ann.blechl@ars.usda.gov FU USDA ARS CRIS [5325-21430-011-00D, 5325-43000-028-00D] FX The authors thank Lydia Li, Stacia Sloane and Jeanie Lin for expert technical assistance. This work was supported by USDA ARS CRIS projects 5325-21430-011-00D and 5325-43000-028-00D. USDA is an equal opportunity provider and employer. Mention of specific product name by the Unites States Department of Agriculture does not constitute an endorsement and does not imply a recommendation over other suitable products. NR 28 TC 0 Z9 0 U1 2 U2 4 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD MAR PY 2016 VL 68 BP 172 EP 180 DI 10.1016/j.jcs.2016.01.009 PG 9 WC Food Science & Technology SC Food Science & Technology GA DJ6ZN UT WOS:000374361800024 ER PT J AU Nararak, J Sathantriphop, S Chauhan, K Tantakom, S Eiden, AL Chareonviriyaphap, T AF Nararak, Jirod Sathantriphop, Sunaiyana Chauhan, Kamal Tantakom, Siripun Eiden, Amanda L. Chareonviriyaphap, Theeraphap TI AVOIDANCE BEHAVIOR TO ESSENTIAL OILS BY ANOPHELES MINIMUS, A MALARIA VECTOR IN THAILAND SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Anopheles minimus; deet; essential oils; excito-repellency ID AEDES-AEGYPTI; INSECT REPELLENTS; EXCITO-REPELLENCY; CULEX-QUINQUEFASCIATUS; MOSQUITO BITES; RESPONSES; RESISTANCE; EFFICACY; THEOBALD; DISEASE AB Essential oils extracted from 4 different plant species-citronella (Cymbopogon nardus), hairy basil (Ocimum americanum), sweet basil (Ocimum basilicum), and vetiver (Vetiveria zizanioides)-were investigated for their irritant and repellent activities against Anopheles minimus, using an excito-repellency test system. Pure essential oils were used in absolute ethanol at the concentrations of 0.5%, 1%, 2.5%, and 5% (v/v) compared with deet. At the lowest concentration of 0.5%, hairy basil displayed the best irritant and repellent effects against An. minimus. Citronella and vetiver at 1-5% showed strong irritant effects with >80% escape, while repellent effects of both oils were observed at 1% and 2.5% citronella (73-89% escape) and at 5% vetiver (83.9% escape). Sweet basil had only moderate irritant action at 5% concentration (69.6% escape) and slightly repellent on test mosquitoes (<50% escape). The results found that hairy basil, citronella, and vetiver are promising potential mosquito repellent products for protection against An. minimus. C1 [Nararak, Jirod; Chareonviriyaphap, Theeraphap] Kasetsart Univ, Fac Agr, Dept Entomol, Bangkok 10900, Thailand. [Sathantriphop, Sunaiyana] Minist Publ Hlth, Dept Med Sci, Natl Inst Hlth, Nonthaburi 11000, Thailand. [Chauhan, Kamal] USDA ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, Beltsville, MD 20705 USA. [Tantakom, Siripun] Kasetsart Univ, Fac Agr, Dept Entomol, Nakhon Pathom 73140, Thailand. [Eiden, Amanda L.] Kasetsart Univ, Fac Agr, Int Programs Trop Agr, Bangkok 10900, Thailand. RP Chareonviriyaphap, T (reprint author), Kasetsart Univ, Fac Agr, Dept Entomol, Bangkok 10900, Thailand. FU Thailand Research Fund (TRF) Organization [RTA5580002]; Center of Agricultural Biotechnology (CAB); International Research Network (IRN) Program, TRF, Thailand [IRN58W0003] FX This research was supported by the Thailand Research Fund (TRF) Organization (Senior Research Scholar: RTA5580002), the Center of Agricultural Biotechnology (CAB), and the International Research Network (IRN) Program (IRN58W0003), TRF, Thailand. NR 33 TC 0 Z9 1 U1 2 U2 3 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD MAR PY 2016 VL 32 IS 1 BP 34 EP 43 PG 10 WC Entomology SC Entomology GA DJ9CA UT WOS:000374510400004 PM 27105214 ER PT J AU Teske, ME Thistle, HW Bonds, JAS AF Teske, Milton E. Thistle, Harold W. Bonds, Jane A. S. TI REBUTTAL TO LETTER TO THE EDITOR CONCERNING TESKE ET AL. (2015) SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Letter ID ADULT MOSQUITO MANAGEMENT; DISPERSION MODEL C1 [Teske, Milton E.] Continuum Dynam Inc, 34 Lexington Ave, Ewing, NJ 08618 USA. [Thistle, Harold W.] US Forest Serv, USDA, 180 Canfield St, Morgantown, WV 26505 USA. [Bonds, Jane A. S.] Bonds Consulting Grp, 3900 Wasp St, Panama City Beach, FL 32408 USA. RP Teske, ME (reprint author), Continuum Dynam Inc, 34 Lexington Ave, Ewing, NJ 08618 USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD MAR PY 2016 VL 32 IS 1 BP 66 EP 67 PG 2 WC Entomology SC Entomology GA DJ9CA UT WOS:000374510400010 PM 27105220 ER EF