FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Hewitt, RE Hollingsworth, TN Chapin, FS Taylor, DL AF Hewitt, Rebecca E. Hollingsworth, Teresa N. Chapin, F. Stuart, III Taylor, D. Lee TI Fire-severity effects on plant-fungal interactions after a novel tundra wildfire disturbance: implications for arctic shrub and tree migration SO BMC ECOLOGY LA English DT Article DE Alnus viridis; Arctic tundra; ARISA; Climate change; Fire severity; Fungal internal transcribed spacer (ITS); Picea mariana; Shrub expansion; Treeline ID BOREAL FOREST; ECTOMYCORRHIZAL FUNGI; CLIMATE-CHANGE; SEWARD PENINSULA; INTERIOR ALASKA; COMMUNITIES; VEGETATION; ESTABLISHMENT; TEMPERATURE; RESILIENCE AB Background: Vegetation change in high latitude tundra ecosystems is expected to accelerate due to increased wildfire activity. High-severity fires increase the availability of mineral soil seedbeds, which facilitates recruitment, yet fire also alters soil microbial composition, which could significantly impact seedling establishment. Results: We investigated the effects of fire severity on soil biota and associated effects on plant performance for two plant species predicted to expand into Arctic tundra. We inoculated seedlings in a growth chamber experiment with soils collected from the largest tundra fire recorded in the Arctic and used molecular tools to characterize root-associated fungal communities. Seedling biomass was significantly related to the composition of fungal inoculum. Biomass decreased as fire severity increased and the proportion of pathogenic fungi increased. Conclusions: Our results suggest that effects of fire severity on soil biota reduces seedling performance and thus we hypothesize that in certain ecological contexts fire-severity effects on plant-fungal interactions may dampen the expected increases in tree and shrub establishment after tundra fire. C1 [Hewitt, Rebecca E.; Chapin, F. Stuart, III; Taylor, D. Lee] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Hewitt, Rebecca E.] No Arizona Univ, Ctr Ecosyst Sci & Soc, POB 5620, Flagstaff, AZ 86011 USA. [Hollingsworth, Teresa N.] US Forest Serv, Pacific Northwest Res Stn, Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. [Taylor, D. Lee] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. RP Hewitt, RE (reprint author), Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA.; Hewitt, RE (reprint author), No Arizona Univ, Ctr Ecosyst Sci & Soc, POB 5620, Flagstaff, AZ 86011 USA. EM rebecca.hewitt@nau.edu FU Alaska EPSCoR [EPS-0701898]; state of Alaska, a National Science Foundation Graduate Research Fellowship [DGE-0639280, 1242789]; Center for Global Change student award FX This research was supported by funding from Alaska EPSCoR (EPS-0701898) and the state of Alaska, a National Science Foundation Graduate Research Fellowship (DGE-0639280 and 1242789), and a Center for Global Change student award to R.E.H. In-kind support was provided by the Bonanza Creek Long-Term Ecological Research site, U.S. Forest Service PNW Research Station, University of Alaska Fairbanks Institute of Arctic Biology greenhouses, and Bureau of Land Management. We thank Randi Jandt, Eric Miller, and David Yokel for field and logistical support, John Alden for our seed source, and Jamie Hollingsworth for creating Fig. 1. NR 61 TC 0 Z9 0 U1 16 U2 31 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1472-6785 J9 BMC ECOL JI BMC Ecol. PD MAY 11 PY 2016 VL 16 AR 25 DI 10.1186/s12898-016-0075-y PG 11 WC Ecology SC Environmental Sciences & Ecology GA DM0AI UT WOS:000376004400001 PM 27169473 ER PT J AU Correa, EA Dayan, FE Owens, DK Rimando, AM Duke, SO AF Correa, Elza Alves Dayan, Franck E. Owens, Daniel K. Rimando, Agnes M. Duke, Stephen O. TI Glyphosate-Resistant and Conventional Canola (Brassica napus L.) Responses to Glyphosate and Aminomethylphosphonic Acid (AMPA) Treatment SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE aminomethylphosphonic acid; AMPA; Brassica nap us; canola; 5-enolpyruvylshikimic acid-3-phosphate synthase; EPSPS; glyphosate; glyphosate oxidase ID SOYBEAN GLYCINE-MAX; CROP TECHNOLOGY; DOSE-RESPONSE; ACCUMULATION; CHLOROPHYLL; METABOLISM; OXIDASE; EVOLUTION; RESIDUES; PROTEIN AB Glyphosate-resistant (GR) canola contains two transgenes that impart resistance to the herbicide glyphosate: (1) the microbial glyphosate oxidase gene (gox) encoding the glyphosate oxidase enzyme (GOX) that metabolizes glyphosate to aminomethylphosphonic acid (AMPA) and (2) cp4 that encodes a GR form of the glyphosate target enzyme 5-enolpyruvylshikimic acid-3-phosphate synthase. The objectives of this research were to determine the phytotoxicity of AMPA to canola, the relative metabolism of glyphosate to AMPA in GR and conventional non-GR (NGR) canola, and AMPA pool sizes in glyphosate-treated GR canola. AMPA applied at 1.0 kg ha(-1) was not phytotoxic to GR or NGR. At this AMPA application rate, NGR canola accumulated a higher concentration of AMPA in its tissues than GR canola. At rates of 1 and 3.33 kg ae ha(-1) of glyphosate, GR canola growth was stimulated. This stimulatory effect is similar to that of much lower doses of glyphosate on NGR canola. Both shikimate and AMPA accumulated in tissues of these glyphosate-treated plants. In a separate experiment in which young GR and NGR canola plants were treated with non-phytotoxic levels of [C-14]-glyphosate, very little glyphosate was metabolized in NGR plants, whereas most of the glyphosate was metabolized to AMPA in GR plants at 7 days after application. Untreated leaves of GR plants accumulated only metabolites (mostly AMPA) of glyphosate, indicating that GOX activity is very high in the youngest leaves. These data indicate that more glyphosate is transformed to AMPA rapidly in GR canola and that the accumulated AMPA is not toxic to the canola plant. C1 [Correa, Elza Alves] Univ Estadual Paulista, UNESP, Campus Registro SP,Rua Nelson Brihi Badur 430, BR-11900000 Registro, SP, Brazil. [Dayan, Franck E.; Owens, Daniel K.; Rimando, Agnes M.; Duke, Stephen O.] USDA ARS, Nat Prod Utilizat Res Unit, POB 1848, University, MS 38677 USA. RP Duke, SO (reprint author), USDA ARS, Nat Prod Utilizat Res Unit, POB 1848, University, MS 38677 USA. EM stephen.duke@ars.usda.gov OI Dayan, Franck/0000-0001-6964-2499 FU Sao Paulo Research Foundation (FAPESP) FX The sabbatical of E. Correa at the USDA, ARS, Natural Product Utilization Research Unit, was funded by the Sao Paulo Research Foundation (FAPESP). NR 35 TC 1 Z9 1 U1 4 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 11 PY 2016 VL 64 IS 18 BP 3508 EP 3513 DI 10.1021/acs.jafc.6b00446 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DL9ND UT WOS:000375969100004 PM 27092715 ER PT J AU Feng, YZ Zhang, L Fu, JM Li, FD Wang, L Tan, XF Mo, WJ Cao, HP AF Feng, Yanzhi Zhang, Lin Fu, Jianmin Li, Fangdong Wang, Lu Tan, Xiaofeng Mo, Wenjuan Cao, Heping TI Characterization of Glycolytic Pathway Genes Using RNA-Seq in Developing Kernels of Eucommia ulmoides SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Eucommia ulmoides; glycolytic pathway; a-linolenic acid; quantitative real-time PCR (qPCR); RNA-seq; transcriptome; unigene ID RUBBER-PRODUCING PLANT; TRANS-POLYISOPRENE; EXPRESSION; OLIVER; TRANSCRIPTOME; BIOSYNTHESIS; ALDOLASE; CHLOROPLAST; HEXOKINASE; METABOLISM AB Eucommia ulmoides Oliver, the only member of the Eucommiaceae family, is a rare and valuable tree used to produce a highly valued traditional Chinese medicine and contains a-linolenic acid (ALA) up to 60% of the total fatty acids in the kernels (embryos). Glycolysis provides both cellular energy and the intermediates for other biosynthetic processes. However, nothing was known about the molecular basis of the glycolytic pathway in E. ulmoides kernels. The purposes of this study were to identify novel genes of E. ulmoides related to glycolytic metabolism and to analyze the expression patterns of selected genes in the kernels. Transcriptome sequencing based on the Illumina platform generated 96,469 unigenes in four cDNA libraries constructed using RNAs from 70 and 160 days after flowering kernels of both low- and high-ALA varieties. We identified and characterized the digital expression of 120 unigenes coding for 24 protein families involved in kernel glycolytic pathway. The expression levels of glycolytic genes were generally higher in younger kernels than in more mature kernels. Importantly, several unigenes from kernels of the high-ALA variety were expressed more than those from the low-ALA variety. The expression of 10 unigenes encoding key enzymes in the glycolytic pathway was validated by qPCR using RNAs from six kernel stages of each variety. The qPCR data were well consistent with their digital expression in transcriptomic analyses. This study identified a comprehensive set of genes for glycolytic metabolism and suggests that several glycolytic genes may play key roles in ALA accumulation in the kernels of E. ulmoides. C1 [Feng, Yanzhi; Fu, Jianmin; Li, Fangdong; Wang, Lu] State Forestry Adm, Paulownia Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China. [Feng, Yanzhi; Zhang, Lin; Tan, Xiaofeng] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China. [Mo, Wenjuan] Chinese Acad Forestry, Forestry Expt Ctr North China, Beijing 102300, Peoples R China. [Cao, Heping] USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. RP Zhang, L (reprint author), Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.; Cao, HP (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. EM triwoodtim918@126.com; Heping.Cao@ars.usda.gov FU National Natural Science Foundation of China [31370682]; USDA-ARS Quality and Utilization of Agricultural Products Research Program 306 through CRIS [6435-41000-102-00D]; NCBI under bioproject [PRJNA314294, SAMN04531657, SRR3203265, SRR3203241, SAMN04531658, SRR3203266, SRR3203268] FX This work was supported by the National Natural Science Foundation of China (31370682) and USDA-ARS Quality and Utilization of Agricultural Products Research Program 306 through CRIS 6435-41000-102-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The RAW data are accessible at NCBI under bioproject (PRJNA314294), biosample for Huazhong No_6 (SAMN04531657): S1 (SRR3203265) and S2 (SRR3203241); biosample for Huazhong No_10 (SAMN04531658): S3 (SRR3203266) and S4 (SRR3203268). NR 42 TC 0 Z9 0 U1 2 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD MAY 11 PY 2016 VL 64 IS 18 BP 3712 EP 3731 DI 10.1021/acs.jafc.5b05918 PG 20 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DL9ND UT WOS:000375969100028 PM 27074598 ER PT J AU Guedira, M Xiong, M Hao, YF Johnson, J Harrison, S Marshall, D Brown-Guedira, G AF Guedira, Mohammed Xiong, Mai Hao, Yuan Feng Johnson, Jerry Harrison, Steve Marshall, David Brown-Guedira, Gina TI Heading Date QTL in Winter Wheat (Triticum aestivum L.) Coincide with Major Developmental Genes VERNALIZATION1 and PHOTOPERIOD1 SO PLOS ONE LA English DT Article ID PSEUDO-RESPONSE-REGULATOR; SPRING GROWTH HABIT; FLOWERING TIME; CULTIVARS; CEREALS; BARLEY; IDENTIFICATION; ADAPTABILITY; REQUIREMENT; IMPROVEMENT AB In wheat (Triticum aestivum L.), time from planting to spike emergence is influenced by genes controlling vernalization requirement and photoperiod response. Characterizing the available genetic diversity of known and novel alleles of VERNALIZATION1 (VRN1) and PHOTOPERIOD1 (PPD1) in winter wheat can inform approaches for breeding climate resilient cultivars. This study identified QTL for heading date (HD) associated with multiple VRN1 and PPD1 loci in a population developed from a cross between two early flowering winter wheat cultivars. When the population was grown in the greenhouse after partial vernalization treatment, major heading date QTLs co-located with the VRN-A1 and VRN-B1 loci. Copy number variation at the VRN-A1 locus influenced HD such that RIL having three copies required longer cold exposure to transition to flowering than RIL having two VRN-A1 copies. Sequencing vrn-B1 winter alleles of the parents revealed multiple polymorphisms in the first intron that were the basis of mapping a major HD QTL coinciding with VRN-B1. A 36 bp deletion in the first intron of VRN-B1 was associated with earlier HD after partial vernalization in lines having either two or three haploid copies of VRN-A1. The VRN1 loci interacted significantly and influenced time to heading in field experiments in Louisiana, Georgia and North Carolina. The PPD1 loci were significant determinants of heading date in the fully vernalized treatment in the greenhouse and in all field environments. Heading date QTL were associated with alleles having large deletions in the upstream regions of PPD-A1 and PPD-D1 and with copy number variants at the PPD-B1 locus. The PPD-D1 locus was determined to have the largest genetic effect, followed by PPD-A1 and PPD-B1. Our results demonstrate that VRN1 and PPD1 alleles of varying strength allow fine tuning of flowering time in diverse winter wheat growing environments. C1 [Guedira, Mohammed; Xiong, Mai; Marshall, David; Brown-Guedira, Gina] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Hao, Yuan Feng; Johnson, Jerry] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. [Harrison, Steve] Louisiana State Univ, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. [Marshall, David; Brown-Guedira, Gina] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Brown-Guedira, G (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA.; Brown-Guedira, G (reprint author), USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM Gina.Brown-Guedira@ars.usda.gov FU United States Department of Agriculture National Institute of Food and Agriculture (nifa.usda.gov) Triticeae Coordinated Agricultural Project [2011-68002-30029] FX Funding was provided by the United States Department of Agriculture National Institute of Food and Agriculture (nifa.usda.gov) Triticeae Coordinated Agricultural Project (grant No. 2011-68002-30029) to GBG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 0 Z9 0 U1 9 U2 14 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 10 PY 2016 VL 11 IS 5 AR e0154242 DI 10.1371/journal.pone.0154242 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM8BN UT WOS:000376585700024 PM 27163605 ER PT J AU Liu, CY Long, JM Zhu, KJ Liu, LL Yang, W Zhang, HY Li, L Xu, Q Deng, XX AF Liu, Chaoyang Long, Jianmei Zhu, Kaijie Liu, Linlin Yang, Wei Zhang, Hongyan Li, Li Xu, Qiang Deng, Xiuxin TI Characterization of a Citrus R2R3-MYB Transcription Factor that Regulates the Flavonol and Hydroxycinnamic Acid Biosynthesis SO SCIENTIFIC REPORTS LA English DT Article ID BLOOD ORANGE JUICES; PHENYLPROPANOID BIOSYNTHESIS; FRUIT-DEVELOPMENT; GENE-EXPRESSION; ARABIDOPSIS; TOMATO; ACCUMULATION; SYNTHASE; SINENSIS; PATHWAY AB Flavonols and hydroxycinnamic acids are important phenylpropanoid metabolites in plants. In this study, we isolated and characterized a citrus R2R3-MYB transcription factor CsMYBF1, encoding a protein belonging to the flavonol-specific MYB subgroup. Ectopic expression of CsMYBF1 in tomato led to an up-regulation of a series of genes involved in primary metabolism and the phenylpropanoid pathway, and induced a strong accumulation of hydroxycinnamic acid compounds but not the flavonols. The RNAi suppression of CsMYBF1 in citrus callus caused a down-regulation of many phenylpropanoid pathway genes and reduced the contents of hydroxycinnamic acids and flavonols. Transactivation assays indicated that CsMYBF1 activated several promoters of phenylpropanoid pathway genes in tomato and citrus. Interestingly, CsMYBF1 could activate the CHS gene promoter in citrus, but not in tomato. Further examinations revealed that the MYBPLANT cis-elements were essential for CsMYBF1 in activating phenylpropanoid pathway genes. In summary, our data indicated that CsMYBF1 possessed the function in controlling the flavonol and hydroxycinnamic acid biosynthesis, and the regulatory differences in the target metabolite accumulation between two species may be due to the differential activation of CHS promoters by CsMYBF1. Therefore, CsMYBF1 constitutes an important gene source for the engineering of specific phenylpropanoid components. C1 [Liu, Chaoyang; Long, Jianmei; Zhu, Kaijie; Liu, Linlin; Yang, Wei; Zhang, Hongyan; Xu, Qiang; Deng, Xiuxin] Huazhong Agr Univ, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China. [Li, Li] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Plant Breeding & Genet Sect,Sch Integrat Plant Sc, Ithaca, NY 14853 USA. RP Deng, XX (reprint author), Huazhong Agr Univ, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China. EM xxdeng@mail.hzau.edu.cn FU NSFC [31330066, 31221062]; Ministry of Education of China [IRT13065] FX We thank Andrew C. Allan (The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand) for providing the vectors for Dual Luciferase Assay. We thank research associates at Center for Protein Research(CPR), Huazhong Agricultural University, for technical support in the EMSA experiment. This work was financially supported by NSFC (No. 31330066 and 31221062) and the Ministry of Education of China (IRT13065). NR 56 TC 0 Z9 0 U1 21 U2 38 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 10 PY 2016 VL 6 AR 25352 DI 10.1038/srep25352 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL2AM UT WOS:000375434700001 PM 27162196 ER PT J AU Xie, JL Hse, CY Shupe, TF Pan, H Hu, TX AF Xie, Jiulong Hse, Chung-Yun Shupe, Todd F. Pan, Hui Hu, Tingxing TI Extraction and characterization of holocellulose fibers by microwave-assisted selective liquefaction of bamboo SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE bamboo; fiber; microwave; selective liquefaction ID WHEAT-STRAW; ENZYMATIC-HYDROLYSIS; WOOD; PRETREATMENT; ETHANOL; DELIGNIFICATION; OPTIMIZATION; COMPONENTS; OXIDATION; GLYCEROL AB Microwave-assisted selective liquefaction was proposed and used as a novel method for the isolation of holocellulose fibers. The results showed that the bamboo lignin component and extractives were almost completely removed by using a liquefaction process at 120 degrees C for 9min, and the residual lignin and extractives in the solid residue were as low as 0.65% and 0.49%, respectively. Increasing the reaction temperature or time could decrease the solid yield, but they can also enhance the removal of lignin and extractives from bamboo particles and increase the holocellulose content in the solid residue. The absorbance bands that characterized functional groups of lignin on the Fourier transform infrared spectra of the solid residue weakened or disappeared. The solid residue showed high crystallinity, indicating the removal of noncellulosic material. Small cracks were observed on the SEM images of the residue, which indicated that the fibers from liquefaction may be susceptible to chemical access or enzyme attack. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43394. C1 [Xie, Jiulong; Hu, Tingxing] Sichuan Agr Univ, Coll Forestry, Dept Wood Sci, Chengdu 611130, Sichuan, Peoples R China. [Hse, Chung-Yun] Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Xie, Jiulong; Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Louisiana Forest Prod Dev Ctr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Pan, Hui] Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China. RP Pan, H (reprint author), Nanjing Forestry Univ, Coll Chem Engn, Nanjing 210037, Jiangsu, Peoples R China. EM hpan@njfu.edu.cn NR 28 TC 2 Z9 2 U1 10 U2 59 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD MAY 10 PY 2016 VL 133 IS 18 AR 43394 DI 10.1002/app.43394 PG 8 WC Polymer Science SC Polymer Science GA DD5WX UT WOS:000369996600024 ER PT J AU Hancock, JF Sooriyapathirana, SS Bassll, NV Stegmeir, T Cai, LC Finn, CE Van de Weg, E Weebadde, CK AF Hancock, James F. Sooriyapathirana, Suneth S. Bassll, Nahla V. Stegmeir, Travis Cai, Lichun Finn, Chad E. Van de Weg, Eric Weebadde, Cholani K. TI Public Availability of a Genotyped Segregating Population May Foster Marker Assisted Breeding (MAB) and Quantitative Trait Loci (QTL) Discovery: An Example Using Strawberry SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Fragaria x ananassa; F. chiloensis; F. virginiana; association mapping; quantitative trait analysis (QTL) ID F VIRGINIANA; CHILOENSIS; SELECTION AB Much of the cost associated with marker discovery for marker assisted breeding (MAB) can be eliminated if a diverse, segregating population is generated, genotyped, and made available to the global breeding community. Herein, we present an example of a hybrid, wild-derived family of the octoploid strawberry that can be used by other breeding programs to economically find and tag useful genes for MAB. A pseudo test cross population between two wild species of Fragaria virginiana and F. chiloensis (FVC 11) was generated and evaluated for a set of phenotypic traits. A total of 106 individuals in the FVC 11 were genotyped for 29,251 single nucleotide polymorphisms (SNPs) utilizing a commercially available, genome-wide scanning platform (Affymetrix Axiom IStraw90(TW)). The marker trait associations were deduced using TASSEL software. The FVC 11 population segregating for daughters per mother, inflorescence number, inflorescence height, crown production, flower number, fruit size, yield, internal color, soluble solids, fruit firmness, and plant vigor. Coefficients of variations ranged from 10% for fruit firmness to 68% for daughters per mother, indicating an underlying quantitative inheritance for each trait. A total of 2,474 SNPs were found to be polymorphic in FVC 11 and strong marker trait associations were observed for vigor, daughters per mother, yield and fruit weight. These data indicate that FVC 11 can be used as a reference population for quantitative trait loci detection and subsequent MAB across different breeding programs and geographical locations. C1 [Hancock, James F.; Cai, Lichun] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Sooriyapathirana, Suneth S.] Univ Peradeniya, Fac Sci, Dept Mol Biol & Biotechnol, Peradeniya, Sri Lanka. [Bassll, Nahla V.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR USA. [Stegmeir, Travis] Lassen Canyon Nursery Breeding Program, Redding, CA USA. [Finn, Chad E.] USDA ARS, Hort Crops Res Unit, Coivallis, OR USA. [Van de Weg, Eric] Wageningen UR Plant Breeding, Wageningen, Netherlands. [Weebadde, Cholani K.] Michigan State Univ, Dept Pant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Hancock, JF (reprint author), Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. EM hancock@msu.edu RI Van de Weg, Eric/G-3225-2011 OI Van de Weg, Eric/0000-0002-9443-5974 FU USDAs National Institute of Food and Agriculture "Specialty Crop Research Initiative project"; RosBREED: Enabling marker-assisted breeding in Rosaceae [2009-51181-05808]; AgBioResearch, Michigan State University, East Lansing FX Partially funded by USDAs National Institute of Food and Agriculture "Specialty Crop Research Initiative project", "RosBREED: Enabling marker-assisted breeding in Rosaceae" (2009-51181-05808) and AgBioResearch, Michigan State University, East Lansing. NR 12 TC 0 Z9 0 U1 6 U2 15 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD MAY 9 PY 2016 VL 7 AR 619 DI 10.3389/fpls.2016.00619 PG 4 WC Plant Sciences SC Plant Sciences GA DL0SW UT WOS:000375343900001 PM 27242825 ER PT J AU Hamelin, FM Allen, LJS Prendeville, HR Hajimorad, MR Jeger, MJ AF Hamelin, Frederic M. Allen, Linda J. S. Prendeville, Holly R. Hajimorad, M. Reza Jeger, Michael J. TI The evolution of plant virus transmission pathways SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article DE Horizontal; Vertical; Seed; Pollen; Vector; Adaptive dynamics; Frequency-dependent; Density-dependent; Evolutionary branching; Evolutionary bi-stability ID CUCUMBER-MOSAIC-VIRUS; DEPENDENT DISEASE TRANSMISSION; PAPAYA-RINGSPOT-VIRUS; SEED TRANSMISSION; MOTTLE-VIRUS; VERTICAL TRANSMISSION; COMPETITIVE ABILITY; VECTOR-TRANSMISSION; APHID TRANSMISSION; LATENT-VIRUS AB The evolution of plant virus transmission pathways is studied through transmission via seed, pollen, or a vector. We address the questions: under what circumstances does vector transmission make pollen transmission redundant? Can evolution lead to the coexistence of multiple virus transmission pathways? We restrict the analysis to an annual plant population in which reproduction through seed is obligatory. A semi-discrete model with pollen, seed, and vector transmission is formulated to investigate these questions. We assume vector and pollen transmission rates are frequency-dependent and density-dependent, respectively. An ecological stability analysis is performed for the semi-discrete model and used to inform an evolutionary study of trade-offs between pollen and seed versus vector transmission. Evolutionary dynamics critically depend on the shape of the trade-off functions. Assuming a trade-off between pollen and vector transmission, evolution either leads to an evolutionarily stable mix of pollen and vector transmission (concave trade-off) or there is evolutionary bistability (convex trade-off); the presence of pollen transmission may prevent evolution of vector transmission. Considering a trade-off between seed and vector transmission, evolutionary branching and the subsequent coexistence of pollen-borne and vector-borne strains is possible. This study contributes to the theory behind the diversity of plant-virus transmission patterns observed in nature. (C) 2016 Elsevier Ltd All rights reserved. C1 [Hamelin, Frederic M.] Agrocampus Ouest, Dept Ecol, IGEPP UMR1349, F-35042 Rennes, France. [Allen, Linda J. S.] Texas Tech Univ, Dept Math & Stat, Lubbock, TX 79409 USA. [Prendeville, Holly R.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Hajimorad, M. Reza] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37996 USA. [Jeger, Michael J.] Univ London Imperial Coll Sci Technol & Med, Ctr Environm Policy, Div Ecol & Evolut, Ascot SL5 7PY, Berks, England. RP Hamelin, FM (reprint author), Agrocampus Ouest, Dept Ecol, IGEPP UMR1349, F-35042 Rennes, France. EM fhamelin@agrocampus-ouest.fr FU National Science Foundation [DBI-1300426]; University of Tennessee, Knoxville; French National Research Agency (ANR) as part of the "Blanc" program [ANR-13-BSV7-0011]; French National Institute for Agricultural Research (INRA) "Plant Health and the Environment" Division FX This work was assisted through participation in Vector Transmission of Plant Viruses Investigative Workshop at the National Institute for Mathematical and Biological Synthesis, sponsored by the National Science Foundation through NSF Award #DBI-1300426, with additional support from The University of Tennessee, Knoxville.; Thanks to Lou Gross and his team. FMH acknowledges partial funding from the French National Research Agency (ANR) as part of the "Blanc 2013" program (ANR-13-BSV7-0011, FunFit project), and from the French National Institute for Agricultural Research (INRA) "Plant Health and the Environment" Division. We thank the anonymous reviewers for their helpful suggestions. NR 136 TC 0 Z9 0 U1 11 U2 16 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 EI 1095-8541 J9 J THEOR BIOL JI J. Theor. Biol. PD MAY 7 PY 2016 VL 396 BP 75 EP 89 DI 10.1016/j.jtbi.2016.02.017 PG 15 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA DK9FS UT WOS:000375236800007 PM 26908348 ER PT J AU Guo, HB La Clair, JJ Masler, EP O'Doherty, GA Xing, YL AF Guo, Haibing La Clair, James J. Masler, Edward P. O'Doherty, George A. Xing, Yalan TI De novo asymmetric synthesis and biological analysis of the daumone pheromones in Caenorhabditis elegans and in the soybean cyst nematode Heterodera glycines SO TETRAHEDRON LA English DT Article DE Daumone pheromones; De novo asymmetric synthesis; Dauer activity; Hatching effect; Pd-glycosylation ID PALLADIUM-CATALYZED GLYCOSYLATION; PLANT-PARASITIC NEMATODES; DAUER PHEROMONE; METABOLOMICS REVEALS; CHEMICAL-STRUCTURE; LOW-TEMPERATURE; C. ELEGANS; OLIGOSACCHARIDES; HYDROGENATION; BIOSYNTHESIS AB The de novo asymmetric total syntheses of daumone 1, daumone 3 along with 5 new analogs are described. The key steps of our approach are: the diastereoselective palladium catalyzed glycosylation reaction; the Noyori reduction of 2-acetylfuran and an ynone, which introduce the absolute stereo chemistry of the sugar and aglycon portion of daumone; and an Achmatowicz rearrangement, an epoxidation and a ring opening installing the remaining asymmetry of daumone. The synthetic daumones 1 and 3 as well as related analogs were evaluated for dauer activity in Caenorhabditis elegans and for effects on hatching of the related nematode Heterodera glycines. This data provides additional structure activity relationships (SAR) that further inform the study of nematode signaling. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Guo, Haibing] Hubei Univ Sci & Technol, Sch Nucl Technol & Chem & Life Sci, Xianning 437100, Peoples R China. [La Clair, James J.] Xenobe Res Inst, POB 3052, San Diego, CA 92163 USA. [Masler, Edward P.] ARS, Nematol Lab, USDA, NEA, Beltsville, MD 20705 USA. [O'Doherty, George A.] Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA. [Xing, Yalan] William Paterson Univ, Dept Chem, 300 Pompton Rd, Wayne, NJ 07470 USA. RP O'Doherty, GA (reprint author), Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA.; Xing, YL (reprint author), William Paterson Univ, Dept Chem, 300 Pompton Rd, Wayne, NJ 07470 USA. EM g.odoherty@neu.edu; XINGY@Mpunj.edu FU National Institutes of Health [GM09025901]; National Science Foundation [CHE-0749451]; National Nature Science Foundation of China [21202136] FX We are grateful to National Institutes of Health (GM09025901 to G.A.O.), National Science Foundation (CHE-0749451 to G.A.O.), and National Nature Science Foundation of China (21202136) for the support of our research programs. NR 49 TC 2 Z9 2 U1 10 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0040-4020 J9 TETRAHEDRON JI Tetrahedron PD MAY 5 PY 2016 VL 72 IS 18 BP 2280 EP 2286 DI 10.1016/j.tet.2016.03.033 PG 7 WC Chemistry, Organic SC Chemistry GA DK0OA UT WOS:000374610900007 ER PT J AU Mahmoud, MS Abu El-Ezz, NT Abdel-Shafy, S Nassar, SA El Namaky, AH Khalil, WKB Knowles, D Kappmeyer, L Silva, MG Suarez, CE AF Mahmoud, Mona S. Abu El-Ezz, Nadia T. Abdel-Shafy, Sobhy Nassar, Somia A. El Namaky, Amira H. Khalil, Wagdy K. B. Knowles, Don Kappmeyer, Lowell Silva, Marta G. Suarez, Carlos E. TI Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches SO PARASITES & VECTORS LA English DT Article DE Babesia; Theileria; Equine; Nested PCR; Competitive ELISA; IFAT; Hemogram ID COMPETITIVE-INHIBITION ELISA; RHOPTRY-ASSOCIATED PROTEIN-1; CANINE BABESIOSIS; HORSES; PIROPLASMOSIS; PCR; DIAGNOSIS; ISRAEL; TRANSMISSION; SPAIN AB Background: Equine piroplasmosis (EP) caused by Theileria equi, Babesia caballi, or both, contributes to significant economic loss in the equine industry and remains uncontrolled in Egypt. This study focuses on surveying T. equi and B. caballi infections and hematological disorders in equine populations in Egypt. Methods: Theileria equi and B. caballi infections were assessed in blood from 88 horses and 51 donkeys in Egypt using light microscopy, indirect immunofluorescent antibody test (IFAT), nested PCR (nPCR), and competitive-ELISA (cELISA) assays. PCR products were examined for specificity by DNA sequencing. Hematological alterations were evaluated using a standard cell counter. Results: Microscopic analysis revealed EP infection in 11.4 % and 17.8 % of horses and donkeys respectively. IFAT detected 23.9 % and 17.0 % infection of T. equi and B. caballi, respectively, in horses, and 31.4 % of T. equi and B. caballi in donkeys. T. equi cELISA detected 14.8 % and 23.5 % positive horses and donkeys, respectively, but the B. caballi RAP-1-based cELISA failed to detect any positives, a result hypothesized to be caused by sequence polymorphism found in the rap-1 genes. Nested-PCR analysis identified 36.4 % and 43.1 % positive horses and donkeys, respectively for T. equi and it also identified 19.3 % and 15.7 % positive horses and donkeys, respectively for B. caballi. The overall EP incidence found in the population under study was relatively high and comparable regardless of the diagnostic method used (56.8 % using nPCR and 48.9 % using IFAT). Hematologic analysis revealed macrocytic hypochromic anemia and thrombocytopenia in all piroplasma-infected horses. Conclusions: The data confirm relatively high levels of EP, likely causing hematological abnormalities in equines in Egypt, and also suggest the need for an improved serological test to diagnose B. caballi infection in this region. C1 [Mahmoud, Mona S.; Abu El-Ezz, Nadia T.; Abdel-Shafy, Sobhy; Nassar, Somia A.; El Namaky, Amira H.] Natl Res Ctr, Parasitol & Anim Dis Dept, 33 Bohouth St, Giza 12622, Egypt. [Khalil, Wagdy K. B.] Natl Res Ctr, Dept Cell Biol, 33 Bohouth St, Giza 12622, Egypt. [Knowles, Don; Kappmeyer, Lowell; Suarez, Carlos E.] WSU, USDA, ARS, Anim Dis Res Unit, Pullman, WA USA. [Knowles, Don; Silva, Marta G.; Suarez, Carlos E.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP Silva, MG (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. EM marta@vetmed.wsu.edu OI ElNamaky, Amira/0000-0002-0121-9288 FU STDF [6133] FX This work was supported by the scientific project number 6133 funded by Research grants from the STDF. We are thankful to Dr. Chungwon Chung and Dr. Scott Adams, from VMRD (Pullman, WA) for kindly providing the cELISA and IFAT kits used for the serological diagnosis of equine theileriosis and babesiosis, to Paul Lacy for technical help in the development of the nested PCR reactions and to Jacob Laughery for additional technical and editorial help. NR 37 TC 1 Z9 1 U1 1 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD MAY 4 PY 2016 VL 9 AR 260 DI 10.1186/s13071-016-1539-9 PG 10 WC Parasitology SC Parasitology GA DL7PG UT WOS:000375832000001 PM 27146413 ER PT J AU Kawada, R Buffington, ML AF Kawada, Ricardo Buffington, Matthew L. TI A Scalable and Modular Dome Illumination System for Scientific Microphotography on a Budget SO PLOS ONE LA English DT Article ID MICROSCOPE AB A scalable and modular LED illumination dome for microscopic scientific photography is described and illustrated, and methods for constructing such a dome are detailed. Dome illumination for insect specimens has become standard practice across the field of insect systematics, but many dome designs remain expensive and inflexible with respect to new LED technology. Further, a one-size-fits-all dome cannot accommodate the large breadth of insect size encountered in nature, forcing the photographer to adapt, in some cases, to a less than ideal dome design. The dome described here is scalable, as it is based on a isodecahedron, and the template for the dome is available as a downloaded file from the internet that can be printed on any printer, on the photographer's choice of media. As a result, a photographer can afford, using this design, to produce a series of domes of various sizes and materials, and LED ring lights of various sizes and color temperatures, depending on the need. C1 [Kawada, Ricardo] Fundacao Univ Fed Grande Dourados, Programa Posgrad Entomol & Conservacao Biodiversi, Rodovia Dourados Itahum,Km 12,Cidade Univ, BR-79804970 Dourados, MS, Brazil. [Buffington, Matthew L.] USDA ARS, Systemat Entomol Lab, Natl Museum Nat Hist, Smithsonian Inst, 10th & Constitut Ave NW,MRC 168,POB 30712, Washington, DC 20013 USA. RP Kawada, R (reprint author), Fundacao Univ Fed Grande Dourados, Programa Posgrad Entomol & Conservacao Biodiversi, Rodovia Dourados Itahum,Km 12,Cidade Univ, BR-79804970 Dourados, MS, Brazil. EM hyme.neo@gmail.com FU CNPq [151153/2013-2]; Systematic Entomology Laboratory, ARS-USDA FX RK was funded by a scholarship granted by CNPq, process number 151153/2013-2; MB was funded by the Systematic Entomology Laboratory, ARS-USDA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 5 TC 1 Z9 1 U1 2 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 3 PY 2016 VL 11 IS 5 AR e0153426 DI 10.1371/journal.pone.0153426 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL5KK UT WOS:000375675700009 PM 27138573 ER PT J AU Fratamico, PM DebRoy, C Liu, YH Needleman, DS Baranzoni, GM Feng, P AF Fratamico, Pina M. DebRoy, Chitrita Liu, Yanhong Needleman, David S. Baranzoni, Gian Marco Feng, Peter TI Advances in Molecular Serotyping and Subtyping of Escherichia coli SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE Escherichia coli; molecular serotyping; subtyping; detection; identification; whole genome sequencing; O-group; H-type ID ANTIGEN GENE-CLUSTER; O SEROGROUP IDENTIFICATION; FIELD GEL-ELECTROPHORESIS; REAL-TIME PCR; MULTIPLEX-PCR; H-ANTIGEN; EXTRAINTESTINAL INFECTIONS; NUCLEOTIDE POLYMORPHISMS; SEQUENCING DATA; STRAINS AB Escherichia coli plays an important role as a member of the gut microbiota; however, pathogenic strains also exist, including various diarrheagenic E. coli pathotypes and extraintestinal pathogenic E. coli that cause illness outside of the GI-tract. E. cols have traditionally been serotyped using antisera against the ca. 186 O-antigens and 53 H-flagellar antigens. Phenotypic methods, including bacteriophage typing and O- and H- serotyping for differentiating and characterizing E. coli have been used for many years; however, these methods are generally time consuming and not always accurate. Advances in next generation sequencing technologies have made it possible to develop genetic-based subtyping and molecular serotyping methods for E. coli, which are more discriminatory compared to phenotypic typing methods. Furthermore, whole genome sequencing (WGS) of E. coli is replacing established subtyping methods such as pulsed field gel electrophoresis, providing a major advancement in the ability to investigate food-borne disease outbreaks and for trace-back to sources. A variety of sequence analysis tools and bioinformatic pipelines are being developed to analyze the vast amount of data generated by WGS and to obtain specific information such as O- and H-group determination and the presence of virulence genes and other genetic markers. C1 [Fratamico, Pina M.; Needleman, David S.; Baranzoni, Gian Marco] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [DebRoy, Chitrita] Penn State Univ, Dept Vet & Biomed Sci, University Pk, PA 16802 USA. [Feng, Peter] US FDA, Div Microbiol, College Pk, MD USA. RP Fratamico, PM (reprint author), USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. EM pina.fratamico@ars.usda.gov FU Agricultural Research services (ARS) Research Participation Program; DOE [DE-AC05-060R23100] FX This work was supported in part by an appointment to the Agricultural Research services (ARS) Research Participation Program which is administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-060R23100. All opinions expressed in this manuscript are the author's and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. NR 94 TC 5 Z9 5 U1 9 U2 20 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD MAY 3 PY 2016 VL 7 AR 644 DI 10.3389/fmicb.2016.00644 PG 8 WC Microbiology SC Microbiology GA DL1MX UT WOS:000375397400001 PM 27199968 ER PT J AU Marble, SC Prior, SA Runion, GB Torbert, HA Gilliam, CH Fain, GB Sibley, JL Knight, PR AF Marble, S. Christopher Prior, Stephen A. Runion, G. Brett Torbert, H. Allen Gilliam, Charles H. Fain, Glenn B. Sibley, Jeff L. Knight, Patricia R. TI Species and Media Effects on Soil Carbon Dynamics in the Landscape SO SCIENTIFIC REPORTS LA English DT Article ID GREENHOUSE-GAS MITIGATION; WOODY NURSERY CROPS; CLIMATE-CHANGE; CO2 EFFLUX; URBAN TREES; SEQUESTRATION; AGRICULTURE; EMISSIONS; DECOMPOSITION; RESPIRATION AB Three woody shrub species [cleyera (Ternstroemia gymnanthera Thunb. 'Conthery'), Indian hawthorn (Rhaphiolepis indica L.) and loropetalum (Loropetalum chinensis Oliv. 'Ruby')] were container-grown for one growing season in 2008 using either pinebark (industry standard), clean chip residual or WholeTree (derived by-products from the forestry industry) as potting substrates and then transplanted into the landscape in 2008. An Automated Carbon Efflux System was used to continually monitor soil CO2 efflux from December 2010 through November 2011 in each species and substrate combination. Changes in soil carbon (C) levels as a result of potting substrate were assessed through soil sampling in 2009 and 2011 and plant biomass was determined at study conclusion. Results showed that soil CO2-C efflux was similar among all species and substrates, with few main effects of species or substrate observed throughout the study. Soil analysis showed that plots with pinebark contained higher levels of soil C in both 2009 and 2011, suggesting that pinebark decomposes slower than clean chip residual or WholeTree and consequently has greater C storage potential than the two alternative substrates. Results showed a net C gain for all species and substrate combinations; however, plants grown in pinebark had greater C sequestration potential. C1 [Marble, S. Christopher] Univ Florida, IFAS, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. [Prior, Stephen A.; Runion, G. Brett; Torbert, H. Allen] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Gilliam, Charles H.; Fain, Glenn B.; Sibley, Jeff L.] Auburn Univ, Dept Hort, Auburn, AL 36849 USA. [Knight, Patricia R.] Mississippi State Coastal Res & Extens Ctr, Biloxi, MS 39532 USA. RP Marble, SC (reprint author), Univ Florida, IFAS, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. EM marblesc@ufl.edu FU USDA-ARS Floriculture Nursery Research Initiative; Horticulture Research Institute FX Financial support for this research was provided by the USDA-ARS Floriculture Nursery Research Initiative and the Horticulture Research Institute. The authors wish to acknowledge Barry Dorman, Robert Icenogle and Jerry Carrington for technical assistance. NR 58 TC 0 Z9 0 U1 9 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD MAY 3 PY 2016 VL 6 AR 25210 DI 10.1038/srep25210 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL0FE UT WOS:000375307000001 PM 27140321 ER PT J AU Rodriguez-Rubio, L Gutierrez, D Donovan, DM Martinez, B Rodriguez, A Garcia, P AF Rodriguez-Rubio, Lorena Gutierrez, Diana Donovan, David M. Martinez, Beatriz Rodriguez, Ana Garcia, Pilar TI Phage lytic proteins: biotechnological applications beyond clinical antimicrobials SO CRITICAL REVIEWS IN BIOTECHNOLOGY LA English DT Review DE Biotechnology; vaccines; pathogen detection; endolysins; food safety; virion-associated peptidoglycan hydrolases; nanotechnology; disinfection ID RESISTANT STAPHYLOCOCCUS-AUREUS; BACTERIAL-CELL WALL; FATAL PNEUMOCOCCAL PNEUMONIA; LACTIC-ACID BACTERIA; GROUP-A STREPTOCOCCI; GROUP-B STREPTOCOCCI; BACILLUS-ANTHRACIS; LISTERIA-MONOCYTOGENES; ESCHERICHIA-COLI; ENDOLYSIN CPL-1 AB Most bacteriophages encode two types of cell wall lytic proteins: endolysins (lysins) and virion-associated peptidoglycan hydrolases. Both enzymes have the ability to degrade the peptidoglycan of Gram-positive bacteria resulting in cell lysis when they are applied externally. Bacteriophage lytic proteins have a demonstrated potential in treating animal models of infectious diseases. There has also been an increase in the study of these lytic proteins for their application in areas such as food safety, pathogen detection/diagnosis, surfaces disinfection, vaccine development and nanotechnology. This review summarizes the more recent developments, outlines the full potential of these proteins to develop new biotechnological tools and discusses the feasibility of these proposals. C1 [Rodriguez-Rubio, Lorena; Gutierrez, Diana; Martinez, Beatriz; Rodriguez, Ana; Garcia, Pilar] CSIC, Inst Prod Lacteos Asturias, Dept Technol & Biotechnol Dairy Prod, DairySafe Grp, Villaviciosa, Asturias, Spain. [Donovan, David M.] USDA ARS, Anim Biosci & Biotechnol Lab, BARC, Beltsville, MD USA. RP Garcia, P (reprint author), CSIC, IPLA, Villaviciosa 33300, Asturias, Spain. EM pgarcia@ipla.csic.es RI Martinez, Beatriz/H-2953-2012; Garcia, Pilar/H-2544-2012; Rodriguez-Rubio, Lorena/D-2888-2017 OI Martinez, Beatriz/0000-0001-7692-1963; Garcia, Pilar/0000-0003-1213-8165; Rodriguez-Rubio, Lorena/0000-0002-7846-4791 FU Ministry of Science and Innovation, Spain [AGL2012-40194-C02-01]; BARD [IS-4573-12R] FX This research study was supported by grants AGL2012-40194-C02-01 (Ministry of Science and Innovation, Spain) (PG) and BARD # IS-4573-12R (DMD). DG is a fellow of the Ministry of Science and Innovation, Spain. Mentioning of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 122 TC 5 Z9 5 U1 12 U2 36 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0738-8551 EI 1549-7801 J9 CRIT REV BIOTECHNOL JI Crit. Rev. Biotechnol. PD MAY 3 PY 2016 VL 36 IS 3 BP 542 EP 552 DI 10.3109/07388551.2014.993587 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DI9JP UT WOS:000373819200013 PM 25603721 ER PT J AU Pyo, J Ha, S Pachepsky, YA Lee, H Ha, R Nam, G Kim, MS Im, J Cho, KH AF Pyo, JongCheol Ha, SeongHyeon Pachepsky, Yakov A. Lee, Hyuk Ha, Rim Nam, Gibeom Kim, Moon S. Im, Jungho Cho, Kyung Hwa TI Chlorophyll-a concentration estimation using three difference bio- optical algorithms, including a correction for the low-concentration range: the case of the Yiam reservoir, Korea SO REMOTE SENSING LETTERS LA English DT Article ID REMOTE-SENSING TECHNIQUES; COASTAL WATERS; INLAND WATERS; LAKE; EUTROPHICATION; PHYTOPLANKTON; QUALITY; MODEL; TELEDETECTION; RETRIEVALS AB Bio-optical algorithms have been applied to monitor water quality in surface water systems. Empirical algorithms have been applied to estimate the chlorophyll-a (chl-a) concentrations. However, the performance of each algorithm severely degrades at concentrations notably lower than 10mgm(-3). This could be attributed to the chl-a specific absorption coefficient that became less consistent at low chl-a concentrations. Nonetheless, no effort has been made in previous studies to correct existing algorithms. In this study, we propose a correction approach to improve their performance for chl-a estimation in Yiam reservoir, Korea. Estimated chl-a concentrations of the algorithms improved after applying the correction process proposed in this study; Nash-Sutcliffe efficiency values increased from 53% to 65% and root mean square error decreased from 39% to 43%, respectively. Further research is needed to verify the correction approaches for different years or study sites. C1 [Pyo, JongCheol; Ha, SeongHyeon; Im, Jungho; Cho, Kyung Hwa] Ulsan Natl Inst Sci & Technol, Dept Urban & Environm Engn, 50 UNIST Gil, Ulsan 689798, South Korea. [Pachepsky, Yakov A.; Kim, Moon S.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD USA. [Lee, Hyuk; Ha, Rim; Nam, Gibeom] Natl Inst Environm Res, Water Qual Assessment Res Div, Environm Res Complex, Inchon, South Korea. RP Im, J; Cho, KH (reprint author), Ulsan Natl Inst Sci & Technol, Dept Urban & Environm Engn, 50 UNIST Gil, Ulsan 689798, South Korea. EM ersgis@unist.ac.kr; khcho@unist.ac.kr FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2014R1A1A2059680]; Environmental Research and Technology Development Fund of the National Institute of Environmental Research, Republic of Korea [1900-1946-342-210] FX This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [grant number NRF-2014R1A1A2059680] and in part by the Environmental Research and Technology Development Fund of the National Institute of Environmental Research, Republic of Korea [grant number 1900-1946-342-210]. NR 45 TC 0 Z9 0 U1 4 U2 28 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 2150-704X EI 2150-7058 J9 REMOTE SENS LETT JI Remote Sens. Lett. PD MAY 3 PY 2016 VL 7 IS 5 BP 407 EP 416 DI 10.1080/2150704X.2016.1142680 PG 10 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA DE0KO UT WOS:000370314000001 ER PT J AU Schuelke, TA Westbrook, A Broders, K Woeste, K MacManes, MD AF Schuelke, Taruna A. Westbrook, Anthony Broders, Kirk Woeste, Keith MacManes, Matthew D. TI De novo genome assembly of Geosmithia morbida, the causal agent of thousand cankers disease SO PEERJ LA English DT Article DE Pathogenesis; Black walnut; Forest pathogen; Walnut twig beetle; De novo genome assembly; Geosmithia morbida ID BEETLE PITYOPHTHORUS-JUGLANDIS; MOUNTAIN PINE-BEETLE; MYCOSPHAERELLA-GRAMINICOLA; CLIMATE-CHANGE; SEQUENCE DATA; WALNUT; ASSOCIATION; DIVERSITY; PATHOGENS; EFFECTOR AB Geosmithia morbida is a filamentous ascomycete that causes thousand cankers disease in the eastern black walnut tree. This pathogen is commonly found in the western U.S.; however, recently the disease was also detected in several eastern states where the black walnut lumber industry is concentrated. G. morbida is one of two known phytopathogens within the genus Geosmithia, and it is vectored into the host tree via the walnut twig beetle. We present the first de novo draft genome of G. morbida. It is 26.5 Mbp in length and contains less than 1% repetitive elements. The genome possesses an estimated 6,273 genes, 277 of which are predicted to encode proteins with unknown functions. Approximately 31.5% of the proteins in G. morbida are homologous to proteins involved in pathogenicity, and 5.6% of the proteins contain signal peptides that indicate these proteins are secreted. Several studies have investigated the evolution of pathogenicity in pathogens of agricultural crops; forest fungal pathogens are often neglected because research efforts are focused on food crops. G. morbida is one of the few tree phytopathogens to be sequenced, assembled and annotated. The first draft genome of G. morbida serves as a valuable tool for comprehending the underlying molecular and evolutionary mechanisms behind pathogenesis within the Geosmithia genus. C1 [Schuelke, Taruna A.; MacManes, Matthew D.] Univ New Hampshire, Dept Mol Cellular & Biomed Sci, Durham, NH 03824 USA. [Westbrook, Anthony] Univ New Hampshire, Dept Comp Sci, Durham, NH 03824 USA. [Broders, Kirk] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Woeste, Keith] US Forest Serv, Hardwood Tree Improvement & Regenerat Ctr, USDA, W Lafayette, IN USA. RP Broders, K (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM kirk.Broders@colostate.edu OI MacManes, Matthew/0000-0002-2368-6960 FU New Hampshire Agricultural Experiment Station; USDA Forest Service, Forest Health and Protection FX Partial funding was provided by the New Hampshire Agricultural Experiment Station. Funding was also provided by the USDA Forest Service, Forest Health and Protection. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 1 Z9 1 U1 9 U2 12 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD MAY 2 PY 2016 VL 4 AR e1952 DI 10.7717/peerj.1952 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DM6UO UT WOS:000376488300002 PM 27168971 ER PT J AU Elder, JR Chiok, KL Paul, NC Haldorson, G Guard, J Shah, DH AF Elder, Jacob R. Chiok, Kim Lam Paul, Narayan C. Haldorson, Gary Guard, Jean Shah, Devendra H. TI The Salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens SO GUT PATHOGENS LA English DT Article DE Salmonella; Salmonella pathogenicity island; Salmonella Enteritidis; Pathogenesis; SPI-13; Host-specificity; Chicken; Streptomycin pre-treated mice ID ENTERICA SEROVAR ENTERITIDIS; INTESTINAL COLONIZATION; SECRETION SYSTEMS; VIRULENCE FACTORS; MOUSE MODEL; INFECTION; GENES; IDENTIFICATION; MACROPHAGES; GENOME AB Background: Salmonella enterica serovar Enteritidis (S. Enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. Salmonella pathogenicity islands (SPIs) are horizontally acquired genomic segments known to contribute to Salmonella pathogenesis. The objective of the current study was to determine the contribution of SPI-13 to S. Enteritidis pathogenesis. Methods: We deleted the entire SPI-13 (Delta SPI-13) from the genome of S. Enteritidis CDC_2010K_0968 strain isolated from a human patient during the 2010 egg-associated outbreak in the US. The kinetics of infection of the wild-type parent and the Delta SPI-13 were compared in orally challenged day-old chickens and streptomycin pre-treated mice. The degree of intestinal inflammation and the survival of mutant strain within the avian (HD11) and murine (RAW264.7) macrophages were also determined. Results: The deletion of the SPI-13 resulted in impaired infection kinetics of S. Enteritidis in streptomycin pre-treated mice which was characterized by significantly lower (P < 0.05) viable counts in the ceca, liver and spleen, impaired ability to induce intestinal inflammation and reduced survival within murine macrophages. Conversely, there were no significant differences in the infection kinetics of Delta SPI-13 in day-old chickens in any of the organs tested and the survival of Delta SPI-13 within chicken macrophages remained unaltered. Conclusions: The results of this study show that SPI-13 contributes to the pathogenesis of S. Enteritidis in streptomycin pre-treated mice but not in day-old chickens and raises the possibility that SPI-13 may play a role in pathogenesis and the host adaptation/restriction of Salmonella serovars. C1 [Elder, Jacob R.; Chiok, Kim Lam; Paul, Narayan C.; Haldorson, Gary; Shah, Devendra H.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Shah, Devendra H.] Washington State Univ, Coll Vet Med, Paul Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Guard, Jean] USDA ARS, Egg Qual & Safety Res Unit, Athens, GA 30605 USA. RP Shah, DH (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA.; Shah, DH (reprint author), Washington State Univ, Coll Vet Med, Paul Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM dshah@vetmed.wsu.edu OI Paul, Narayan/0000-0003-2674-8693; Shah, Devendra/0000-0001-6365-7868 FU funds the Agricultural Animal Health Program, College of Veterinary Medicine, Washington State University; Poncin Trust Fellowship FX This project was funded in part with the funds the Agricultural Animal Health Program, College of Veterinary Medicine, Washington State University. Jacob Elder was supported by Poncin Trust Fellowship. NR 34 TC 2 Z9 2 U1 0 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1757-4749 J9 GUT PATHOG JI Gut Pathogens PD MAY 2 PY 2016 VL 8 AR 16 DI 10.1186/s13099-016-0098-0 PG 12 WC Gastroenterology & Hepatology; Microbiology SC Gastroenterology & Hepatology; Microbiology GA DL5TU UT WOS:000375700600001 PM 27141235 ER PT J AU Capsel, RT Thoen, CO Reinhardt, TA Lippolis, JD Olsen, R Stabel, JR Bannantine, JP AF Capsel, Randal T. Thoen, Charles O. Reinhardt, Timothy A. Lippolis, John D. Olsen, Renee Stabel, Judith R. Bannantine, John P. TI Composition and Potency Characterization of Mycobacterium avium subsp paratuberculosis Purified Protein Derivatives SO PLOS ONE LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; INTERFERON-GAMMA ASSAY; SECRETED ANTIGENS; SKIN-TEST; BOVINE PARATUBERCULOSIS; JOHNES-DISEASE; DAIRY HERDS; DIAGNOSIS; CATTLE; TESTS AB Mycobacterium avium subsp. paratuberculosis (MAP) purified protein derivatives (PPDs) are immunologic reagents prepared from cultured filtrates of the type strain. Traditional production consists of floating culture incubation at 37 degrees C, organism inactivation by autoclaving, coarse filtration, and protein precipitation. Three traditional production PPDs were used in this study including lot 9801, which served as a reference and has been used in the field for decades. Alternative production PPDs (0902A and 0902B), in which the autoclaving step was removed, were also analyzed in this study. SDS-PAGE analysis revealed protein smearing in traditional PPDs, but distinct bands were observed in the alternative PPD preparations. Antibody bound distinct protein bands in the alternative PPDs by immunoblot analysis, whereas an immunoreactive smear was observed with the traditional PPDs. Mass spectrometry identified 194 proteins among three PPD lots representing the two different production methods, ten of which were present in all PPDs examined. Selected proteins identified by mass spectrometry were recombinantly expressed and purified from E. coli and evaluated by the guinea pig potency test. Seven recombinant proteins showed greater erythema as compared to the reference PPD lot 9801 in paired guinea pigs and were able to stimulate interferon-gamma production in blood from Johne's positive animals. These results suggest that autoclaving culture suspensions is not a necessary step in PPD production and specific proteins could supplant the PPD antigen for intradermal skin testing procedures and for use as in-vitro assay reagents. C1 [Capsel, Randal T.] USDA, Natl Vet Serv Labs, AHPIS, Ames, IA 50010 USA. [Thoen, Charles O.] Iowa State Univ, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA USA. [Reinhardt, Timothy A.; Lippolis, John D.; Stabel, Judith R.; Bannantine, John P.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. [Olsen, Renee] USDA, Ctr Vet Biol, APHIS, Ames, IA USA. RP Bannantine, JP (reprint author), USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. EM john.bannantine@ars.usda.gov OI Bannantine, John/0000-0002-5692-7898 FU USDA, the Agricultural Research Service; USDA, the Animal and Plant Health Inspection Service FX This work was funded by two agencies within the USDA, the Agricultural Research Service and the Animal and Plant Health Inspection Service. NR 33 TC 0 Z9 0 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD MAY 2 PY 2016 VL 11 IS 5 AR e0154685 DI 10.1371/journal.pone.0154685 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL5KB UT WOS:000375674800041 PM 27136199 ER PT J AU Vereecken, H Schnepf, A Hopmans, JW Javaux, M Or, D Roose, DOT Vanderborght, J Young, MH Amelung, W Aitkenhead, M Allison, SD Assouline, S Baveye, P Berli, M Bruggemann, N Finke, P Flury, M Gaiser, T Govers, G Ghezzehei, T Hallett, P Franssen, HJH Heppell, J Horn, R Huisman, JA Jacques, D Jonard, F Kollet, S Lafolie, F Lamorski, K Leitner, D McBratney, A Minasny, B Montzka, C Nowak, W Pachepsky, Y Padarian, J Romano, N Roth, K Rothfuss, Y Rowe, EC Schwen, A Simunek, J Tiktak, A Van Dam, J van der Zee, SEATM Vogel, HJ Vrugt, JA Wohling, T Young, IM AF Vereecken, H. Schnepf, A. Hopmans, J. W. Javaux, M. Or, D. Roose, T. Vanderborght, J. Young, M. H. Amelung, W. Aitkenhead, M. Allison, S. D. Assouline, S. Baveye, P. Berli, M. Brueggemann, N. Finke, P. Flury, M. Gaiser, T. Govers, G. Ghezzehei, T. Hallett, P. Franssen, H. J. Hendricks Heppell, J. Horn, R. Huisman, J. A. Jacques, D. Jonard, F. Kollet, S. Lafolie, F. Lamorski, K. Leitner, D. McBratney, A. Minasny, B. Montzka, C. Nowak, W. Pachepsky, Y. Padarian, J. Romano, N. Roth, K. Rothfuss, Y. Rowe, E. C. Schwen, A. Simunek, J. Tiktak, A. Van Dam, J. van der Zee, S. E. A. T. M. Vogel, H. J. Vrugt, J. A. Woehling, T. Young, I. M. TI Modeling Soil Processes: Review, Key Challenges, and New Perspectives SO VADOSE ZONE JOURNAL LA English DT Review ID ROOT WATER-UPTAKE; SEDIMENT TRANSPORT MODELS; DIFFUSE-REFLECTANCE SPECTROSCOPY; ARBUSCULAR MYCORRHIZAL FUNGI; MULTIPLE ECOSYSTEM SERVICES; SATURATED-UNSATURATED FLOW; GROUND-PENETRATING RADAR; SYNTHETIC-APERTURE RADAR; DIGITAL ELEVATION MODEL; ORGANIC-MATTER DYNAMICS AB The remarkable complexity of soil and its importance to a wide range of ecosystem services presents major challenges to the modeling of soil processes. Although major progress in soil models has occurred in the last decades, models of soil processes remain disjointed between disciplines or ecosystem services, with considerable uncertainty remaining in the quality of predictions and several challenges that remain yet to be addressed. First, there is a need to improve exchange of knowledge and experience among the different disciplines in soil science and to reach out to other Earth science communities. Second, the community needs to develop a new generation of soil models based on a systemic approach comprising relevant physical, chemical, and biological processes to address critical knowledge gaps in our understanding of soil processes and their interactions. Overcoming these challenges will facilitate exchanges between soil modeling and climate, plant, and social science modeling communities. It will allow us to contribute to preserve and improve our assessment of ecosystem services and advance our understanding of climate-change feedback mechanisms, among others, thereby facilitating and strengthening communication among scientific disciplines and society. We review the role of modeling soil processes in quantifying key soil processes that shape ecosystem services, with a focus on provisioning and regulating services. We then identify key challenges in modeling soil processes, including the systematic incorporation of heterogeneity and uncertainty, the integration of data and models, and strategies for effective integration of knowledge on physical, chemical, and biological soil processes. We discuss how the soil modeling community could best interface with modern modeling activities in other disciplines, such as climate, ecology, and plant research, and how to weave novel observation and measurement techniques into soil models. We propose the establishment of an international soil modeling consortium to coherently advance soil modeling activities and foster communication with other Earth science disciplines. Such a consortium should promote soil modeling platforms and data repository for model development, calibration and intercomparison essential for addressing contemporary challenges. C1 [Vereecken, H.; Schnepf, A.; Vanderborght, J.; Amelung, W.; Brueggemann, N.; Franssen, H. J. Hendricks; Huisman, J. A.; Jonard, F.; Kollet, S.; Montzka, C.; Rothfuss, Y.] Forschungszentrum Julich, Inst Biogeosci, IBG 3, Agrosphere Inst, D-52425 Julich, Germany. [Vereecken, H.; Vanderborght, J.; Franssen, H. J. Hendricks; Huisman, J. A.; Kollet, S.] Forschungszentrum Julich, Ctr High Performance Sci Comp Terr Syst, HPSC TerrSys, Geoverbund ABC J, D-52425 Julich, Germany. [Hopmans, J. W.] Univ Calif Davis, Coll Agr & Environm Sci, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Javaux, M.] Catholic Univ Louvain, Earth & Life Inst, Environm Sci, Croix Sud 2,L7-05-02, B-1348 Louvain, Belgium. [Or, D.] ETH, Soil & Terr Environm Phys, Univ Str 16,CHN F 29-1-8092, Zurich, Switzerland. [Roose, T.; Heppell, J.] Univ Southampton, Fac Engn & Environm, Bioengn Sci Res Grp, Univ Rd, Southampton SO17 1BJ, Hants, England. [Young, M. H.] Univ Texas Austin, Jackson Sch Geosci, Bur Econ Geol, Austin, TX 78712 USA. [Amelung, W.] Univ Bonn, INRES, Inst Crop Sci & Resource Conservat Soil Sci & Soi, Nussallee 13, D-53115 Bonn, Germany. [Aitkenhead, M.] James Hutton Inst, Aberdeen AB15 8QH, Scotland. [Allison, S. D.] Univ Calif Irvine, Sch Biol Sci, Ecol & Evolutionary Biol, Irvine, CA 92717 USA. [Assouline, S.] Agr Res Org, Volcani Ctr, Dept Environm Phys & Irrigat, Inst Soils Water & Environm Sci, POB 6, IL-50250 Bet Dagan, Israel. [Baveye, P.] Univ Paris Saclay, INRA, AgroParisTech, Unite EcoSys, Ave Lucien Bretignieres, F-78850 Thiverval Grignon, France. [Berli, M.] Desert Res Inst, Div Hydrol Sci, 755 E Flamingo Rd, Las Vegas, NV 89119 USA. [Finke, P.] Univ Ghent, Dept Soil Sci & Geol, Krijgslaan 281 WE13, B-9000 Ghent, Belgium. [Flury, M.] Washington State Univ, Dept Crop & Soil Sci, Phys Vadose Zone Hydrol, 2606 W Pioneer, Puyallup, WA 98371 USA. [Gaiser, T.] Univ Bonn, Inst Crop Sci & Resource Conservat, Katzenburgweg 5, D-53115 Bonn, Germany. [Govers, G.] Katholieke Univ Leuven, Div Geog, Dept Earth & Environm Sci, Celestijnenlaan 200E, B-3001 Louvain, Belgium. [Ghezzehei, T.] Sch Nat Sci, Life & Environm Sci, 5200 North Lake Rd, Merced, CA 95343 USA. [Hallett, P.] Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen AB24 3UU, Scotland. [Horn, R.] Inst Plant Nutr & Soil Sci, Hermann Rodewaldstr 2, D-24118 Kiel, Germany. [Jacques, D.] Belgian Nucl Res Ctr SCK CEN, Inst Environm Hlth & Safety, Mol, Belgium. [Lafolie, F.] INRA, EMMAH UMR1114, F-84914 Avignon 9, France. [Lamorski, K.] Polish Acad Sci, Inst Agrophys, Doswiadczalna Str 4, PL-20290 Lublin, Poland. [Leitner, D.] Univ Vienna, Computat Sci Ctr, Oskar Morgenstern Pl 1, A-1090 Vienna, Austria. [McBratney, A.; Minasny, B.; Padarian, J.] Univ Sydney, Dept Environm Sci, Sydney, NSW 2006, Australia. [Nowak, W.] Univ Stuttgart, Inst Wasser & Umweltsystemmodellierung IWS, Lehrstuhl Stochast Simulat & Sicherheitsforsch Hy, Stuttgart, Germany. [Pachepsky, Y.] USDA ARS, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Romano, N.] Univ Naples Federico II, Dept Agron, AFBE Div, Via Univ 100, Naples, Italy. [Roth, K.] Inst Environm Phys, Neuenheimer Feld 229, D-69120 Heidelberg, Germany. [Rowe, E. C.] Environm Ctr Wales, Ctr Ecol & Hydrol, Deiniol Rd, Bangor LL57 2UW, Gwynedd, Wales. [Schwen, A.] Inst Hydraul & Landeskulturelle Wasserwirtschaft, Nussdorfer Lande 11, A-1190 Vienna, Austria. [Simunek, J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Tiktak, A.] PBL Netherlands Environm Assessment Agcy, Leeuwenhoeklaan 9,3721 MA,POB 303, NL-3720 AH Bilthoven, Netherlands. [Van Dam, J.; van der Zee, S. E. A. T. M.] WU Environm Sci Soil Phys & Land Management, POB 47, NL-6700 AA Wageningen, Netherlands. [van der Zee, S. E. A. T. M.] Monash Univ, Sch Chem, Melbourne, Vic 3800, Australia. [Vogel, H. J.] UFZ, Dept Soil Phys, Theodor Lieser Str 4, D-06120 Halle, Germany. [Vrugt, J. A.] Univ Calif Irvine, Dept Civil & Environm Engn, Irvine, CA USA. [Vrugt, J. A.] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA USA. [Vrugt, J. A.] Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1012 WX Amsterdam, Netherlands. [Woehling, T.] Tech Univ Wohling Dresden, Dept Hydrol, D-01069 Dresden, Germany. [Woehling, T.] Univ Tubingen, Inst Geosci, Water & Earth Syst Sci WESS Competence Cluster, D-72076 Tubingen, Germany. [Woehling, T.] Lincoln Agritech Ltd, Ruakura Res Ctr, Hamilton 3240, New Zealand. [Young, I. M.] Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia. RP Vereecken, H (reprint author), Forschungszentrum Julich, Inst Biogeosci, IBG 3, Agrosphere Inst, D-52425 Julich, Germany.; Vereecken, H (reprint author), Forschungszentrum Julich, Ctr High Performance Sci Comp Terr Syst, HPSC TerrSys, Geoverbund ABC J, D-52425 Julich, Germany. EM h.vereecken@fz-juelich.de RI Vogel, Hans-Jorg/J-9845-2016; Or, Dani/D-8768-2012; Rowe, Edwin/A-7641-2011; Romano, Nunzio/H-9038-2012; finke, peter/G-2197-2013; Allison, Steven/E-2978-2010; Javaux, Mathieu/D-8200-2012; Huisman, J.A. (Sander)/I-7078-2012; Schnepf, Andrea/F-5203-2015; OI Huisman, Johan Alexander/0000-0002-1327-0945; Jacques, Diederik/0000-0001-9393-2963; Vogel, Hans-Jorg/0000-0003-2404-9485; Or, Dani/0000-0002-3236-2933; Romano, Nunzio/0000-0001-7276-6994; finke, peter/0000-0003-3037-1665; Allison, Steven/0000-0003-4629-7842; Javaux, Mathieu/0000-0002-6168-5467; Schnepf, Andrea/0000-0003-2203-4466; Vanderborght, Jan/0000-0001-7381-3211; Minasny, Budiman/0000-0002-1182-2371; Montzka, Carsten/0000-0003-0812-8570; Rothfuss, Youri/0000-0002-8874-5036 NR 691 TC 13 Z9 13 U1 21 U2 21 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD MAY PY 2016 VL 15 IS 5 DI 10.2136/vzj2015.09.0131 PG 57 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DP2PW UT WOS:000378332400003 ER PT J AU Wang, Y Davidson, I Fouchier, R Spackman, E AF Wang, Yue Davidson, Irit Fouchier, Ron Spackman, Erica TI Antigenic Cartography of H9 Avian Influenza Virus and Its Application to Vaccine Selection SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE low pathogenic avian influenza virus; H9N2 influenza; poultry disease; antigenic cartography ID CONTINUING EVOLUTION; SOUTHEASTERN CHINA; A VIRUSES; CHICKENS; ISRAEL; POULTRY; IRAN; HEMAGGLUTININ; ANTIBODIES; CHALLENGE AB Vaccination is frequently used as a control method for the H9 subtype of low pathogenicity avian influenza virus (AIV), which is widespread in Asia and the Middle East. One of the most important factors for selecting an effective vaccine strain is the antigenic match between the hemagglutinin protein of the vaccine and the strain circulating in the field. To demonstrate the antigenic relationships among H9 AIVs, with a focus on Israeli H9 isolates, antigenic cartography was used to develop a map of H9 AIVs. Based on their antigenic diversity, three isolates from Israel were selected for vaccination-challenge studies: 1) the current vaccine virus, A/chicken/Israel/215/2007 H9N2 (Ck/215); 2) A/chicken/Israel/1163/2011 H9N2 (Ck/1163); and 3) A/ostrich/Israel/1436/2003 (Os/1436). A 50% infective dose (ID50) model was used to determine the effect of the vaccines on susceptibility to infection by using a standardized dose of vaccine. Sera collected immediately prior to challenge showed that Ck/215 was the most immunogenic, followed by Ck/1163 and Os/1436. A significant difference in ID50 was only observed with Ck/215 homologous challenge, where the ID50 was increased by 2 log 10 per bird. The ID50 for Ck/1163 was the same, regardless of vaccine, including sham vaccination. The ID50 for Os/1436 was above the maximum possible dose and therefore could not be established. C1 [Wang, Yue; Spackman, Erica] ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Davidson, Irit] Kimron Vet Inst, POB 12, IL-50250 Bet Dagan, Israel. [Fouchier, Ron] Erasmus MC, Dept Virosci, POB 2040, NL-3000 CA Rotterdam, Netherlands. RP Spackman, E (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Erica.Spackman@ARS.USDA.GOV RI Fouchier, Ron/A-1911-2014 OI Fouchier, Ron/0000-0001-8095-2869 NR 32 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 218 EP 225 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900016 ER PT J AU Kilgore, MA Snyder, SA AF Kilgore, Michael A. Snyder, Stephanie A. TI Exploring the relationship between parcelization metrics and natural resource managers' perceptions of forest land parcelization intensity SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Exploded logit; Parcellation; Parcelization; Forest ownership; Subdivision; Ranked data ID STAKEHOLDERS; NORTHWOODS; VALUES AB A major challenge associated with forest land parcelization, defined as the subdivision of forest land holdings into smaller ownership parcels, is that little information exists on how to measure its severity and judge its impacts across forest landscapes. To address this information gap, an on-line survey presented field-based public natural resource managers in the Lake States of Minnesota, Wisconsin, and Michigan with four private forest ownership patterns, each containing the same total forest area, number of parcels, and average parcel size. Survey respondents ranked each landscape from most to least parcelized based on the degree to which each ownership pattern was perceived to adversely impact three forest-based goods and services: timber production, recreational access, and wildlife habitat. Using an exploded logit model, respondents' rankings of parcelization impact were found to be consistent, regardless of the forest good or service evaluated. Rankings were also not influenced by the respondent's professional discipline, location, length of professional experience, or employer. Of the four parcelization metrics evaluated, the Gini Coefficient and Adjusted Mean metrics appear to best capture the forest land ownership patterns that natural resource professionals are most concerned about, suggesting those metrics may be useful indicators by which to assess parcelization impact. (C) 2016 Elsevier B.V. All rights reserved. C1 [Kilgore, Michael A.] Univ Minnesota, Coll Food Agr & Nat Resource Sci, Dept Forest Resources, 301K Green Hall,1530 Cleveland Ave North, St Paul, MN 55108 USA. [Snyder, Stephanie A.] USDA Forest Serv, Northern Res Stn, Operat Res Analyst, 1992 Flowell Ave, St Paul, MN 55108 USA. RP Kilgore, MA (reprint author), Univ Minnesota, Coll Food Agr & Nat Resource Sci, Dept Forest Resources, 301K Green Hall,1530 Cleveland Ave North, St Paul, MN 55108 USA. EM mkilgore@umn.edu; stephaniesnyder@fs.fed.us FU USDA Forest Service, Northern Research Station; University of Minnesota's Agricultural Experiment Station [42-054] FX The authors wish to thank Carl-Philipp Petri and Steve Taff for their assistance in data collection and data analysis. Funding for this research was provided by the USDA Forest Service, Northern Research Station and the University of Minnesota's Agricultural Experiment Station (project 42-054). NR 26 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD MAY PY 2016 VL 149 BP 43 EP 48 DI 10.1016/j.landurbplan.2016.02.003 PG 6 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA EE8HQ UT WOS:000389866700005 ER PT J AU DeHaan, LR Van Tassel, DL Anderson, JA Asselin, SR Barnes, R Baute, GJ Cattani, DJ Culman, SW Dorn, KM Hulke, BS Kantar, M Larson, S Marks, MD Miller, AJ Poland, J Ravetta, DA Rude, E Ryan, MR Wyse, D Zhang, XF AF DeHaan, Lee R. Van Tassel, David L. Anderson, James A. Asselin, Sean R. Barnes, Richard Baute, Gregory J. Cattani, Douglas J. Culman, Steve W. Dorn, Kevin M. Hulke, Brent S. Kantar, Michael Larson, Steve Marks, M. David Miller, Allison J. Poland, Jesse Ravetta, Damian A. Rude, Emily Ryan, Matthew R. Wyse, Don Zhang, Xiaofei TI A Pipeline Strategy for Grain Crop Domestication SO CROP SCIENCE LA English DT Article ID EASTERN NORTH-AMERICA; PERENNIAL-GRAIN; PLANT DOMESTICATION; ILLINOIS BUNDLEFLOWER; RICE DOMESTICATION; GENETIC-ANALYSIS; PROVIDES TOOLS; FOOD; PRODUCTIVITY; WHEATGRASS AB In the interest of diversifying the global food system, improving human nutrition, and making agriculture more sustainable, there have been many proposals to domesticate wild plants or complete the domestication of semidomesticated orphan crops. However, very few new crops have recently been fully domesticated. Many wild plants have traits limiting their production or consumption that could be costly and slow to change. Others may have fortuitous preadaptations that make them easier to develop or feasible as high-value, albeit low-yielding, crops. To increase success in contemporary domestication of new crops, we propose a pipeline approach, with attrition expected as species advance through the pipeline. We list criteria for ranking domestication candidates to help enrich the starting pool with more pre-adapted, promising species. We also discuss strategies for prioritizing initial research efforts once the candidates have been selected: developing higher value products and services from the crop, increasing yield potential, and focusing on overcoming undesirable traits. Finally, we present new-crop case studies that demonstrate that wild species' limitations and potential (in agronomic culture, shattering, seed size, harvest, cleaning, hybridization, etc.) are often only revealed during the early phases of domestication. When nearly insurmountable barriers were reached in some species, they have been (at least temporarily) eliminated from the pipeline. Conversely, a few species have moved quickly through the pipeline as hurdles, such as low seed weight or low seed number per head, were rapidly overcome, leading to increased confidence, farmer collaboration, and program expansion. C1 [DeHaan, Lee R.; Van Tassel, David L.] Land Inst, 2440 E Water Well Rd, Salina, KS 67401 USA. [Anderson, James A.; Wyse, Don; Zhang, Xiaofei] Univ Minnesota, Dept Agron & Plant Genet, 411 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Asselin, Sean R.; Cattani, Douglas J.] Univ Manitoba, Dept Plant Sci, 66 Dafoe Rd, Winnipeg, MB R3T 2N2, Canada. [Barnes, Richard] Univ Minnesota, Dept Ecol Evolut & Behav, 100 Ecol Bldg,1987 Upper Buford Circle, St Paul, MN 55108 USA. [Baute, Gregory J.] Univ British Columbia, Dept Bot, 3529-6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada. [Culman, Steve W.] Ohio State Univ, Sch Environm & Nat Resources, 130 Williams Hall,1680 Madison Ave, Wooster, OH 44691 USA. [Dorn, Kevin M.; Marks, M. David] Univ Minnesota, Dept Plant Biol, Biol Sci Ctr 250, 1445 Gortner Ave, St Paul, MN 55108 USA. [Hulke, Brent S.] USDA ARS, Sunflower & Plant Biol Res Unit, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. [Kantar, Michael] Univ British Columbia, Biodivers Res Ctr, 3529-6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada. [Kantar, Michael] Univ British Columbia, Dept Bot, 3529-6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada. [Larson, Steve] USDA ARS Forage & Range Res, 700 N 1100 E, Logan, UT 84322 USA. [Miller, Allison J.] St Louis Univ, Dept Biol, 3507 Laclede Ave, St Louis, MO 63130 USA. [Poland, Jesse] Kansas State Univ, Dept Plant Pathol, 4024 Throckmorton Hall, Manhattan, KS 66506 USA. [Poland, Jesse] Kansas State Univ, Dept Agron, 4024 Throckmorton Hall, Manhattan, KS 66506 USA. [Ravetta, Damian A.] Consejo Nacl Invest Cient & Tecn, Museo Egidio Feruglio, Fontana 140, RA-9100 Trelew, Chubut, Argentina. [Rude, Emily] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Ryan, Matthew R.] Cornell Univ, Sect Soil & Crop Sci, 515 Bradfield Hall, Ithaca, NY 14853 USA. RP DeHaan, LR (reprint author), Land Inst, 2440 E Water Well Rd, Salina, KS 67401 USA. EM dehaan@landinstitute.org OI Kantar, Michael/0000-0001-5542-0975; Poland, Jesse/0000-0002-7856-1399 FU Estes Institute FX This paper was inspired by the Domestication Group discussions at the "New Roots for Ecological Intensification" meeting held 27-31 Oct. 2014 in Estes Park, CO. The authors are grateful to Melinda Merrill and the Estes Institute for sponsoring and hosting the meeting. They also gratefully acknowledge the helpful critiques received from five anonymous reviewers. NR 77 TC 0 Z9 0 U1 9 U2 11 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 917 EP 930 DI 10.2135/cropsci2015.06.0356 PG 14 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600001 ER PT J AU Kurtz, B Gardner, CAC Millard, MJ Nickson, T Smith, JSC AF Kurtz, Bradley Gardner, Candice A. C. Millard, Mark J. Nickson, Thomas Smith, J. Stephen C. TI Global Access to Maize Germplasm Provided by the US National Plant Germplasm System and by US Plant Breeders SO CROP SCIENCE LA English DT Article ID AMERICAN DENT CORN; VARIETY PROTECTION; GENETIC-RESOURCES; AGRICULTURAL SUSTAINABILITY; GREEN-REVOLUTION; DIVERSITY; IMPROVEMENT; PRODUCTIVITY; INBREDS; MARKERS AB Access to high-yielding germplasm increases agricultural productivity. Plant Variety Protection (PVP) is the most globally used form of Intellectual Property Protection. Commercially available varieties protected solely by PVP can be accessed for further breeding within the country or region of protection. Unlike the practice in most other countries, the USDA has a policy of providing parental inbred lines of hybrids into the public domain following expiration of their protection by PVP. Parental lines and varieties protected by utility patents are also available in the public domain following expiration of protection. These distributions represent fresh opportunities for researchers, hybrid seed producers and plant breeders, nationally and internationally. As a contribution to the continuing debate over ownership and access to plant germplasm we report distributions of maize (Zea mays L.) by the US National Plant Germplasm System (NPGS). During 1988-2015 the NPGS distributed 251,926 packets to 7,582 requestors, fulfilling 10,432 orders. Distributions were primarily to US entities (87%), with 32,520 (13%) to a total of 79 foreign countries. Of the total NPGS maize distributions reported here, 45,968 (18%) were expired or off-PVP germplasm. We hope that access provided to this germplasm will act as a reminder that the primary benefit of the Multilateral System under the International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA) is indeed that of having facilitated global access to germplasm and that this concept will remain an important guiding principle during further implementation of the Treaty. C1 [Kurtz, Bradley] DuPont Pioneer, 7300 NW 62nd Ave, Johnston, IA 50131 USA. [Gardner, Candice A. C.; Millard, Mark J.] USDA ARS, North Cent Reg Plant Intro Stn, G212,Agron Bldg, Ames, IA 50011 USA. [Nickson, Thomas] Monsanto Co, 800 North Lindbergh Blvd, St Louis, MO 63167 USA. [Smith, J. Stephen C.] Iowa State Univ, Seed Sci Bldg, Ames, IA 50011 USA. RP Kurtz, B (reprint author), DuPont Pioneer, 7300 NW 62nd Ave, Johnston, IA 50131 USA. EM Bradley.Kurtz@Pioneer.com NR 78 TC 0 Z9 0 U1 3 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 931 EP 941 DI 10.2135/cropsci2015.07.0439 PG 11 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600002 ER PT J AU Ashraf, B Edriss, V Akdemir, D Autrique, E Bonnett, D Crossa, J Janss, L Singh, R Jannink, JL AF Ashraf, Bilal Edriss, Vahid Akdemir, Deniz Autrique, Enrique Bonnett, David Crossa, Jose Janss, Luc Singh, Ravi Jannink, Jean-Luc TI Genomic Prediction using Phenotypes from Pedigreed Lines with No Marker Data SO CROP SCIENCE LA English DT Article ID SINGLE-STEP; ENVIRONMENT INTERACTION; RELATIONSHIP MATRICES; GENETIC EVALUATION; MOLECULAR MARKERS; WIDE SELECTION; FULL PEDIGREE; INFORMATION; GENOTYPE; VALUES AB Until now, genomic prediction (GP) in plant breeding has only used information from individuals that have been genotyped. Information from nongenotyped relatives of genotyped individuals can also be used. Single-step GP combines marker and pedigree information into a single relationship matrix to perform GP. The objective of this study was to evaluate single-step GP in a wheat breeding program. We compared the performance of pedigree-based, marker-based, and single-step models (ABLUP, GBLUP, and HBLUP, respectively). Data consisted of 1176 genotyped (via genotyping-by-sequencing) and 11,131 nongenotyped wheat lines replicated in five management environments at the CIMMYT experiment station in Obregon, Mexico. Analyses involved three scenarios: (i) all lines had pedigree information but only some were genotyped, with phenotypes from one or two environments in the 2011-2012 season, (ii) all lines had genotype and pedigree information and phenotypes from four or five environments in the 2012-2013 season, and (iii) the combination of Scenarios 1 and 2. Prediction accuracies were calculated by fivefold cross validation on plant height, maturity, heading date, and grain yield. The single-step HBLUP outperformed GBLUP and pedigree-based ABLUP in all cases. We conclude that the single-step procedure combining pedigree and genomic marker data should be favored where appropriate data is available for GP in wheat breeding programs. C1 [Ashraf, Bilal; Edriss, Vahid; Janss, Luc] Aarhus Univ, Dept Mol Biol & Genet, Ctr Quantitat Genet & Genom, DK-8830 Tjele, Denmark. [Ashraf, Bilal; Edriss, Vahid; Akdemir, Deniz; Jannink, Jean-Luc] Cornell Univ, Plant Breeding & Genet Sect, Sch Integrat Plant Sci, 240 Emerson Hall, Ithaca, NY 14853 USA. [Autrique, Enrique; Bonnett, David; Crossa, Jose; Singh, Ravi] Int Maize & Wheat Improvement Ctr CIMMYT, Apdo Postal 6-641, El Batan 06600, Mexico. [Jannink, Jean-Luc] USDA ARS, RW Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Jannink, JL (reprint author), Cornell Univ, Plant Breeding & Genet Sect, Sch Integrat Plant Sci, 240 Emerson Hall, Ithaca, NY 14853 USA.; Jannink, JL (reprint author), USDA ARS, RW Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM jeanluc.jannink@ars.usda.gov FU Bill and Melinda Gates Foundation; USDA-NIFA-AFRI Award [2011-68002-30029]; Triticeae Coordinated Agricultural Project FX This research received funds from a grant from the Bill and Melinda Gates Foundation. Deniz Akdemir was supported by USDA-NIFA-AFRI Award 2011-68002-30029, The Triticeae Coordinated Agricultural Project. We thank reviewers for constructive comments that required analyses that improved this study. NR 29 TC 0 Z9 0 U1 4 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 957 EP 964 DI 10.2135/cropsci2015.02.0111 PG 8 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600004 ER PT J AU Cruz, CD Peterson, GL Bockus, WW Kankanala, P Dubcovsky, J Jordan, KW Akhunov, E Chumley, F Baldelomar, FD Valent, B AF Cruz, C. D. Peterson, G. L. Bockus, W. W. Kankanala, P. Dubcovsky, J. Jordan, K. W. Akhunov, E. Chumley, F. Baldelomar, F. D. Valent, B. TI The 2NS Translocation from Aegilops ventricosa Confers Resistance to the Triticum Pathotype of Magnaporthe oryzae SO CROP SCIENCE LA English DT Article ID PYRICULARIA-ORYZAE; WHEAT; GRISEA; GENES; BLAST; PATHOGENICITY; COMMON; BRAZIL; LINES; RUST AB Wheat blast is a serious disease caused by the fungus Magnaporthe oryzae (Triticum pathotype) (MoT). The objective of this study was to determine the effect of the 2NS translocation from Aegilops ventricosa (Zhuk.) Chennav on wheat head and leaf blast resistance. Disease phenotyping experiments were conducted in growth chamber, greenhouse, and field environments. Among 418 cultivars of wheat (Triticum aestivum L.), those with 2NS had 50.4 to 72.3% less head blast than those without 2NS when inoculated with an older MoT isolate under growth chamber conditions. When inoculated with recently collected isolates, cultivars with 2NS had 64.0 to 80.5% less head blast. Under greenhouse conditions when lines were inoculated with an older MoT isolate, those with 2NS had a significant head blast reduction. With newer isolates, not all lines with 2NS showed a significant reduction in head blast, suggesting that the genetic background and/or environment may influence the expression of any resistance conferred by 2NS. However, when near-isogenic lines (NILs) with and without 2NS were planted in the field, there was strong evidence that 2NS conferred resistance to head blast. Results from foliar inoculations suggest that the resistance to head infection that is imparted by the 2NS translocation does not confer resistance to foliar disease. In conclusion, the 2NS translocation was associated with significant reductions in head blast in both spring and winter wheat. C1 [Cruz, C. D.; Bockus, W. W.; Kankanala, P.; Jordan, K. W.; Akhunov, E.; Chumley, F.; Valent, B.] Kansas State Univ, Dept Plant Pathol, 1712 Claflin Rd, Manhattan, KS 66506 USA. [Peterson, G. L.] USDA ARS, Ft Detrick, MD 21702 USA. [Kankanala, P.] Samuel Roberts Noble Fdn Inc, Div Plant Biol, 2510 Sam Noble Pkwy, Ardmore, OK 73401 USA. [Dubcovsky, J.] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. [Dubcovsky, J.] Univ Calif Davis, 1 Shields Ave, Davis, CA 95616 USA. [Baldelomar, F. D.] Asociac Nacl Prod Oleaginosas & Trigo, Av Ovidio Barbery Esq Jaime Mendoza, Santa Cruz, Bolivia. RP Cruz, CD (reprint author), Kansas State Univ, Dept Plant Pathol, 1712 Claflin Rd, Manhattan, KS 66506 USA. EM ccruz@ksu.edu RI Dubcovsky, Jorge/A-4969-2008 OI Dubcovsky, Jorge/0000-0002-7571-4345 FU Agriculture and Food Research Initiative from USDA National Institute of Food and Agriculture [2009-55605-05201, 2011-68002-30029, 2013-68004-20378]; Rotary International; Rotary Foundation; Rotary International District; Manhattan Rotary Club of Manhattan Kansas through Rotary Global Grant Program; Howard Hughes Medical Institute; Gordon Foundation; Betty Moore Foundation FX The project has been funded by Agriculture and Food Research Initiative Competitive Grants No. 2009-55605-05201, 2011-68002-30029 (TCAP) and 2013-68004-20378 (Blast Integrated Project; 'BIP') from the USDA National Institute of Food and Agriculture. Funding was also provided by Rotary International, The Rotary Foundation, Rotary International District 5710, and the Manhattan Rotary Club of Manhattan Kansas, through the Rotary Global Grant Program. Contribution number 15-408-J from the Kansas Agricultural Experiment Station. Dr. Dubcovsky acknowledges support from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundations. NR 35 TC 4 Z9 4 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 990 EP 1000 DI 10.2135/cropsci2015.07.0410 PG 11 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600007 PM 27814405 ER PT J AU Liu, Y Jia, YL Gealy, D Goad, DM Caicedo, AL Olsen, KM AF Liu, Yan Jia, Yulin Gealy, David Goad, David M. Caicedo, Ana L. Olsen, Kenneth M. TI Marker Development for Rice Blast Resistance Gene Pi66(t) and Application in the USDA Rice Mini-Core Collection SO CROP SCIENCE LA English DT Article ID WEEDY RED RICE; ASSISTED SELECTION; DISEASE RESISTANCE; MAGNAPORTHE-ORYZAE; PI-Z; DNA; POPULATION; SATIVA AB Molecular markers are useful for the identification of critical genes controlling agricultural traits of interest in crop germplasm and for the use of these genes in crop improvement using marker-assisted selection (MAS). The improvement of blast disease resistance of rice (Oryza sativa L.) varieties is one of the major goals for rice breeders. The blast resistance (R) gene Pi66(t) was previously mapped to a 129-kb genomic region on chromosome 11, where six candidate R genes were predicted. To help rice breeders in rice blast resistance MAS breeding, we developed three polymerase-chain-reaction (PCR)-based molecular markers, WRKY41, NBS-LRR-970-1, and NBS-LRR-970-2, for the Pi66(t) locus. The validation of two markers, WRKY41 and NBS-LRR-970-1, suggested they cosegregated with the Pi66(t) gene in two recombinant inbred line (RIL) populations. The application of two markers in a USDA-ARS rice mini-core collection indicated that NBS-LRR-970-1 was a Pi66(t)-linked specific marker. The developed marker not only can be used to identify resistant lines with the Pi66(t) gene in mapping populations but also can be used in screening rice germplasm with a broad genetic background. Taken together, these newly developed markers and newly identified rice germplasm lines carrying the Pi66(t) gene will provide essential tools and materials for rice breeders in MAS breeding. C1 [Liu, Yan] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. [Jia, Yulin; Gealy, David] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Goad, David M.; Olsen, Kenneth M.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Caicedo, Ana L.] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA. RP Jia, YL (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM yulin.jia@ars.usda.gov FU National Science Foundation Plant Genome Research Program (NSF) [IOS-1032023] FX We thank Coretta Torrence, Michael Lin, Tracy Bianco, and Melissa H. Jia for their excellent technical support. This project was funded in part by the National Science Foundation Plant Genome Research Program (NSF award IOS-1032023). The USDA is an equal-opportunity provider and employer. NR 26 TC 0 Z9 0 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1001 EP 1008 DI 10.2135/cropsci2015.07.0422 PG 8 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600008 ER PT J AU Fakthongphan, J Graybosch, RA Baenziger, PS AF Fakthongphan, Juthamas Graybosch, R. A. Baenziger, P. S. TI Combining Ability for Tolerance to Pre-Harvest Sprouting in Common Wheat (Triticum aestivum L.) SO CROP SCIENCE LA English DT Article ID WHITE WINTER-WHEAT; QUANTITATIVE TRAIT LOCI; ALPHA-AMYLASE ACTIVITY; FALLING NUMBER; SPRING WHEAT; BREAD-WHEAT; MOLECULAR MARKERS; GRAINED WHEAT; MAJOR QTL; DORMANCY AB Pre-harvest sprouting (PHS) affects wheat (Triticum aestivum L.) yield and end-use product quality leading to massive economic losses. Red wheat cultivars are typically more resistant to PHS than white wheat. The objective of this study was to identify red wheat genotypes capable of donating genes for PHS tolerance to white wheat, independent of red seed coat color. A factorial (M x N) mating was conducted using six white wheat genotypes 'Cayuga', 'Nuplains', NW97S218, NW99L7068, 'Peck', and 'RioBlanco' and eight hard red wheat genotypes 'Arapahoe', CO960293-2, 'Jagalene', NE98466, NI01812, 'Wesley', 'Niobrara', and 'Plainsman V'. General combining ability (GCA) for individual parents and specific combining ability (SCA) for specific crosses were used to identify effective red wheat donors. General combining ability and SCA were calculated from a pre-harvest tolerance score (Lambda) determined after testing heads in a misting chamber and from falling number measurements of field-grown materials. General combining ability among red parents was significant for both Delta and falling number, but not in white parents. General combining ability or SCA by environmental interactions, with the exception of. from GCA in red wheat, were significant. Jagalene and Niobrara were identified as red wheat genetic reservoirs for additional genes of PHS tolerance useful in white wheat breeding. A significant correlation of SCA of falling number and SCA of Delta was detected (r = 0. 38, n = 48, P = 0.007). The falling number assay can be replaced by an assay for Delta to evaluate PHS tolerance in wheat breeding programs in areas in which pre-harvest sprouting is not routinely observed. C1 [Fakthongphan, Juthamas; Baenziger, P. S.] Univ Nebraska, Dept Agron & Hort, 330 Keim Hall, Lincoln, NE 68583 USA. [Fakthongphan, Juthamas] Dept Agr, 50 Phaholyothin Rd, Bangkok 10900, Thailand. [Graybosch, R. A.] Univ Nebraska, USDA ARS, 251 Filley Hall East Campus, Lincoln, NE 68583 USA. [Graybosch, R. A.] Univ Nebraska, Dept Agron & Hort, 251 Filley Hall East Campus, Lincoln, NE 68583 USA. RP Graybosch, RA (reprint author), Univ Nebraska, USDA ARS, 251 Filley Hall East Campus, Lincoln, NE 68583 USA.; Graybosch, RA (reprint author), Univ Nebraska, Dept Agron & Hort, 251 Filley Hall East Campus, Lincoln, NE 68583 USA. EM bob.graybosch@ars.usda.gov NR 55 TC 0 Z9 0 U1 7 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1025 EP 1035 DI 10.2135/cropsci2015.08.0490 PG 11 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600011 ER PT J AU Burke, JJ AF Burke, John J. TI Genetic Diversity of Natural Crossing in Six Cotton Cultivars SO CROP SCIENCE LA English DT Article ID GOSSYPIUM-HIRSUTUM; POLLEN; POLLINATION; GROWTH; FIELD; FLOW AB We have previously shown genetic diversity in mature cotton (Gossypium hirsutum L.) pollen sensitivity to low humidity. In vitro studies showed reduced pollen germination and pollen tube growth under low humidity. The present study investigated the impact of pollen sensitivity to low humidity on the amount of outcrossing to neighboring plants. We utilized "red" and "green" pigmented cotton, in addition to gossypol glanded and glandless cotton cultivars, to study pollen movement in the field. Genetic diversity in outcrossing was observed among the six cultivars tested. Differences in outcrossing of 5 to 15% were observed under both irrigated and dryland production systems. Higher levels of outcrossing were observed within a row compared to outcrossing between rows. Cotton cultivars with mature pollen sensitive to low humidity exhibited reduced outcrossing compare to cultivars that were less sensitive to low humidity. These results suggest that cultivars with pollen sensitive to low humidity can be used to reduce natural outcrossing and subsequently adventitious presence in adjacent fields throughout the Southwest. C1 [Burke, John J.] USDA ARS, Cropping Syst Res Lab, 3810 4th Street, Lubbock, TX 79415 USA. RP Burke, JJ (reprint author), USDA ARS, Cropping Syst Res Lab, 3810 4th Street, Lubbock, TX 79415 USA. EM john.burke@ars.usda.gov FU Cotton Incorporated [5-703] FX The author thanks Jacob Sanchez and DeeDee Laumbach for their excellent technical support. This study was funded in part by Cotton Incorporated project No. 5-703. NR 22 TC 0 Z9 0 U1 3 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1059 EP 1066 DI 10.2135/cropsci2015.09.0572 PG 8 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600014 ER PT J AU Thapa, R Carrero-Colon, M Hudson, KA AF Thapa, Rima Carrero-Colon, Militza Hudson, Karen A. TI New Alleles of FATB1A to Reduce Palmitic Acid Levels in Soybean SO CROP SCIENCE LA English DT Article ID SEED OIL; FATTY-ACIDS; INHERITANCE; IDENTIFICATION; SPECIFICITY; RESIDUES; MUTANTS; TRAITS; LINES; GENE AB In conventional elite soybean [Glycine max (L.) Merr.] varieties palmitic acid typically constitutes 10% of the total seed oil. Palmitic acid is a saturated fat linked to increased cholesterol levels, and reducing levels of saturated fats in soybean oil is a target for soybean improvement. To identify novel and useful variation that could help in reducing palmitic acid levels in soybean, we screened a chemically mutagenized population. Two lines with reduced levels of seed palmitic acid were identified, and each line had a 30% reduction in palmitic acid. Both lines carried distinct mutations in the FATB1A (Glyma. 05G012300) gene that co-segregated with the reduced palmitic acid phenotype. One line carried a single base change that disrupted the splicing of the FATB1A mRNA, and the second contained a missense mutation (aspartic acid substituted for glycine) in a conserved domain of the FATB1 enzyme. Molecular markers were designed that will enable breeders to follow these new FATB1A mutant alleles in plant populations to facilitate gene stacking for oil improvement. C1 [Thapa, Rima] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Carrero-Colon, Militza; Hudson, Karen A.] USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Hudson, KA (reprint author), USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. EM karen.hudson@ars.usda.gov FU USDA-ARS CRIS [3602-110-007D] FX We are grateful to Dr. Kristin Bilyeu for soybean GC analysis and Dr. M. Hudson for critical reading of the manuscript. This work was funded by USDA-ARS CRIS project no. 3602-110-007D. Product names are necessary to report factually on available data. However the USDA neither guarantees nor warrants the standard of the product, and use of the names implies no approval of the product to the exclusion of others that may also be suitable. NR 26 TC 1 Z9 1 U1 2 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1076 EP 1080 DI 10.2135/cropsci2015.09.0597 PG 5 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600016 ER PT J AU Subramanyam, S Nemacheck, JA Xiao, XY McDonald, MJ Williams, CE AF Subramanyam, Subhashree Nemacheck, Jill A. Xiao, Xiangye McDonald, Melissa J. Williams, Christie E. TI Targeted Discovery of Single-Nucleotide Polymorphisms in an Unmarked Wheat Chromosomal Region Containing the Hessian Fly Resistance Gene H33 SO CROP SCIENCE LA English DT Article ID ORYZA-SATIVA L.; BULKED SEGREGANT ANALYSIS; HIGH-DENSITY; WINTER-WHEAT; MARKERS; RICE; POPULATIONS; TRAITS; MAP; SELECTION AB The durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.] Hessian fly [Mayetiola destructor (Say)] resistance gene H33 was previously introgressed into common wheat (T. aestivum L.) and mapped to chromosome 3AS. However, H33 located to a region that is known to be devoid of molecular markers, with the closest flanking simple-sequence repeat (SSR) markers 7.4 and 25.1 cM from the gene. This lack of markers flanking economically important genes is a common problem in self-pollinating crops because of low polymorphism and, in the case of wheat, a large genome size. H33 is currently effective against all virulent Hessian fly populations tested from the southeastern United States, a region where deployed resistance genes are beginning to fail; thus, effort was needed to identify tightly linked flanking markers to use this valuable gene in marker-assisted selection (MAS). To target the 25.1-cM segment adjacent to H33 for marker discovery, eight resistant and four susceptible plants were selected for genotyping-by-sequencing (GBS). Four single-nucleotide polymorphisms (SNPs) were identified, with the closest marker being only 6.7 cM away and converted into high-throughput polymerase chain reaction (PCR)based markers for the Kompetitive Allele Specific PCR (KASP) assay. In addition, endonucleases were identified that cut at the SNPs for restriction profiling as an alternate to the KASP assay. Development of these cost-effective markers provided both low-and high-throughput alternatives for introgressing H33 into wheat cultivars while minimizing linkage drag associated with durum regions of chromosome 3AS. C1 [Subramanyam, Subhashree; Xiao, Xiangye; McDonald, Melissa J.; Williams, Christie E.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. [Nemacheck, Jill A.; Williams, Christie E.] USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Williams, CE (reprint author), Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA.; Williams, CE (reprint author), USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. EM christie.williams@ars.usda.gov FU USDA-ARS CRIS [3602-22000-018-00D-18] FX This work was a joint contribution by the USDA-ARS Crop Production and Pest Control Research Unit and Purdue University. Research was supported by USDA-ARS CRIS number 3602-22000-018-00D-18. Mention of a commercial or a proprietary product does not constitute endorsement or recommendation for its use by the USDA. NR 35 TC 0 Z9 0 U1 6 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1106 EP 1114 DI 10.2135/cropsci2015.10.0630 PG 9 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600019 ER PT J AU Sun, J Ohm, HW Poland, JA Williams, CE AF Sun, Jin Ohm, Herbert W. Poland, Jesse A. Williams, Christie E. TI Mapping Four Quantitative Trait Loci Associated with Type I Fusarium Head Blight Resistance in Winter Wheat 'INW0412' SO CROP SCIENCE LA English DT Article ID MARKER-ASSISTED SELECTION; TRITICUM-AESTIVUM L.; SPRING WHEAT; GIBBERELLA-ZEAE; SCAB RESISTANCE; BREAD WHEAT; DNA MARKERS; QTL; POPULATION; GENES AB Fusarium head blight (FHB) is one of the most damaging wheat (Triticum aestivum L.) diseases in humid and semihumid regions around the world. Breeding efforts have focused on resistance mechanisms that limit the spread once a spike is infected-type II resistance. But resistance to initial infection, type I resistance, is an important trait that could be combined with other modes of resistance. The objective of this project was to identify and map the locations of previously unknown quantitative trait loci (QTL) that reduce the incidence of initial infection. A mapping population of 198 recombinant inbred lines (RILs) was evaluated for type I resistance in both the field and greenhouse. A two-enzyme genotyping-by-sequencing (GBS) approach was applied to construct a 1883-cM linkage map yielding single-nucleotide polymorphism (SNP) markers. Composite interval mapping analysis detected a QTL on chromosome 1AS under greenhouse conditions and three other QTL on chromosomes 1BL, 2BL, and 3AS in field environments. Each QTL explained between 7.44 and 12.20% of the total phenotypic variation. Sixteen RILs were identified with markers for all three QTL on chromosomes 1BL, 2BL, and 3AS exhibiting significantly improved type I resistance (a mean of 33.06% improvement) over RILs with none of the three markers in field experiments. Our results also confirmed that types I and II FHB resistance were controlled by different loci in the wheat RIL population and could be used in combination to provide multiple layers of defense for cultivar development through marker-assisted selection. C1 [Sun, Jin; Ohm, Herbert W.; Williams, Christie E.] Purdue Univ, Dept Agron, 915 West State St, W Lafayette, IN 47907 USA. [Sun, Jin] Cornell Univ, Dept Plant Breeding & Genet, 240 Emerson Hall, Ithaca, NY 14853 USA. [Poland, Jesse A.] Kansas State Univ, Wheat Genet Resource Ctr, Dept Plant Pathol, Manhattan, KS 66506 USA. [Poland, Jesse A.] Kansas State Univ, Dept Agron, Throckmorton Plant Sci Ctr 2004, Manhattan, KS 66506 USA. [Williams, Christie E.] USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Williams, CE (reprint author), Purdue Univ, Dept Agron, 915 West State St, W Lafayette, IN 47907 USA.; Williams, CE (reprint author), USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. EM cwilliams@purdue.edu OI Poland, Jesse/0000-0002-7856-1399 FU USDA National Institute of Food and Agriculture [10-85117-20607, 58-3620-8-688]; Agronomy Department, Purdue University; China Scholarship Council FX This project was funded by USDA National Institute of Food and Agriculture competitive grant number 10-85117-20607 and award number 58-3620-8-688. Mention of a commercial or proprietary product does not constitute endorsement or recommendation for its use by the USDA. The project was also funded by the Agronomy Department, Purdue University, and the China Scholarship Council Fellowship. The authors wish to thank Shuangye Wu (Department of Agronomy, Kansas State University) for assistance during GBS library construction. We appreciate Dr. Jessica Rutkoski (Plant Breeding and Genetics Department, Cornell University) and Erena Edae (Department of Agronomy, Kansas State University) who kindly provided advice for GBS analysis. Thanks to Dr. Greg Hunt (Department of Entomology, Purdue University) for advice on map construction and Dr. Charles Crane (USDA-ARS, Crop Production and Pest Control Research Unit) for assistance with segregation distortion filtering. Nolan Anderson and Dr. Kirstein Wise (Department of Botany and Plant Pathology, Purdue University) provided Fusarium graminiearum isolates. We also thank all Ohm lab staff and graduate and undergraduate students who assisted in FHB incidence data collection and Williams lab staff who assisted with DNA isolation. NR 42 TC 0 Z9 0 U1 6 U2 6 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1163 EP 1172 DI 10.2135/cropsci2015.06.0390 PG 10 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600025 ER PT J AU Zheng, QL Liu, J Goff, BM Dinkins, RD Zhu, HY AF Zheng, Qiaolin Liu, Jinge Goff, Ben M. Dinkins, Randy D. Zhu, Hongyan TI Genetic Manipulation of miR156 for Improvement of Biomass Production and Forage Quality in Red Clover SO CROP SCIENCE LA English DT Article ID BROMIDE SPECTROPHOTOMETRIC METHOD; TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; LIGNIN CONCENTRATION; MICRORNA; SPL3; OVEREXPRESSION; RECOGNITION; SWITCHGRASS; TRANSITION AB Red clover (Trifolium pratense L.) is an important forage legume in the United States. Improving forage quality and biomass yield is an important goal of forage breeding programs. Plant development, particularly the transition from vegetative to reproductive growth, is an important factor affecting biomass accumulation and forage quality. Thus, genetic manipulation of genes that regulate the transition from the vegetative to reproductive phase can be an efficient strategy for genetic improvement of red clover. One such gene is miR156, a microRNA gene that serves as a master regulator of vegetative phase change in land plants. We report here the development of transgenic red clover plants overexpressing the maize Corngrass1 gene encoding two tandem miR156s. The transgenic plants displayed striking phenotypic changes compared with the wild-type plants, including the increased number of shoots, delayed flowering, enhanced forage yield, and improved forage quality. These results suggest that manipulating miR156 or its target genes is a potential tool for genetic improvement of forage legumes. C1 [Zheng, Qiaolin; Liu, Jinge; Goff, Ben M.; Zhu, Hongyan] Univ Kentucky, Dept Plant & Soil Sci, Plant Sci Bldg, Lexington, KY 40546 USA. [Dinkins, Randy D.] Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Agr Sci Ctr North, Lexington, KY 40546 USA. RP Zhu, HY (reprint author), Univ Kentucky, Dept Plant & Soil Sci, Plant Sci Bldg, Lexington, KY 40546 USA. EM hzhu4@uky.edu OI Dinkins, Randy/0000-0002-2127-273X FU USDA-ARS Forage Animal Production Research Unit; College of Agriculture, Food and Environments, University of Kentucky FX We thank Dr. George Chuck of the Plant Gene Expression Center of USDA for providing the binary vector pUbi:Cg1:Nos for this research. We also thank Laura Harris for her help in forage quality analysis, and Dr. Ray Smith for his advice on this project. This project was supported by the Specific Cooperative Agreements (SCA) between the USDA-ARS Forage Animal Production Research Unit and the College of Agriculture, Food and Environments, University of Kentucky. NR 32 TC 0 Z9 0 U1 8 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1199 EP 1205 DI 10.2135/cropsci2015.11.0726 PG 7 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600029 ER PT J AU Similien, A Shannon, DA Wood, CW Rimando, AM Kemppainen, BW van Santen, E Joshee, N AF Similien, Arsene Shannon, Dennis A. Wood, C. Wesley Rimando, Agnes M. Kemppainen, Barbara W. van Santen, Edzard Joshee, Nirmal TI Shade, Irrigation, and Nutrients Affect Flavonoid Concentration and Yield in American Skullcap SO CROP SCIENCE LA English DT Article ID SCUTELLARIA-LATERIFLORA L.; MEDICINAL-PLANT; BROCCOLI; FERTILIZATION; MANAGEMENT; EXTRACTS; DROUGHT; GROWTH; WATER AB American skullcap (Scutellaria lateriflora L.) is valued for its sedative properties that are associated with flavonoids. Information on how growing conditions affect flavonoid content is lacking. A 2 by 2 by 3 factorial experiment was conducted in a randomized complete block design (r = 4) with a split-plot restriction on randomization to explore effects of light, irrigation, and nutrient application on flavonoid concentration and yield in American skullcap. Treatment factors were shade (40% shade vs. no shade), irrigation (applied at 30 kPa vs. no irrigation), and nutrients (no fertilizer vs. fertilizer [100 kg N, 68 kg P, 42 kg K ha(-1)] or chicken litter [100 kg N, 50 kg P, 123 kg K ha(-1)]). Shade formed the main plot units; irrigation and nutrients were randomized within subplots. Aboveground biomass was harvested twice in both 2007 and 2008. Dried, finely ground samples were extracted and analyzed for flavonoid concentration using high-performance liquid chromatography (HPLC). The flavonoid baicalin was present in highest concentration and yield, followed by baicalein; wogonin and chrysin were present in only small amounts. Flavonoid concentration was 72% higher in full sun, 15% higher with irrigation, while added nutrients decreased baicalin concentration by 6% but increased baicalein by 16%. Significant interactions of shade. irrigation and shade. nutrients were also observed. Flavonoid yield was 25% higher under shade, 92% higher with irrigation, and 43% higher with added nutrients. Significant interactions of shade. irrigation, shade. nutrients, and irrigation. nutrients were also observed. Increasing biomass yield had a greater effect on total flavonoid harvested than did increasing flavonoid concentration. C1 [Similien, Arsene] SEED Minist Inc, Div Agr, POB 32, Warf Masse, Les Cayes, Haiti. [Shannon, Dennis A.; van Santen, Edzard] Auburn Univ, Dept Crop Soil & Environm Sci, 202 Funchess Hall, Auburn, AL 36849 USA. [Wood, C. Wesley] Univ Florida, West Florida Res & Educ Ctr, 5988 Highway 90,Bldg 4900, Milton, FL 32583 USA. [Rimando, Agnes M.] USDA ARS, Nat Prod Utilizat Res Unit, POB 8048, Univ, MS 38677 USA. [Kemppainen, Barbara W.] Auburn Univ, Dept Anat Physiol & Pharmacol, Univ, AL 36849 USA. [Joshee, Nirmal] Ft Valley State Univ, Agr Res Stn, Ft Valley, GA 31030 USA. RP Shannon, DA (reprint author), Auburn Univ, Dept Crop Soil & Environm Sci, 202 Funchess Hall, Auburn, AL 36849 USA. EM shannda@auburn.edu NR 38 TC 0 Z9 0 U1 7 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X EI 1435-0653 J9 CROP SCI JI Crop Sci. PD MAY-JUN PY 2016 VL 56 IS 3 BP 1213 EP 1224 DI 10.2135/cropsci2015.03.0186 PG 12 WC Agronomy SC Agriculture GA DX9YY UT WOS:000384754600031 ER PT J AU Bregitzer, P Hu, GS Marshall, JM Raboy, V AF Bregitzer, Phil Hu, Gongshe Marshall, Juliet M. Raboy, Victor TI Registration of 'Harriman' Low-Phytate, Hulled Spring Barley SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID HORDEUM-VULGARE L.; PHYTIC-ACID; SEED PHOSPHORUS; MUTATIONS; LINES AB 'Harriman' (Reg. No. CV-358, PI 676261) is a hulled, low-phytate barley (Hordeum vulgare L.) cultivar, the second to be developed and released by the USDA-ARS. Compared to the previously released hulled, low-phytate cultivar, 'Herald', Harriman has higher grain yield, higher test weight, higher inorganic phosphorus (P-i), and lower phytate phosphorus (P). This cultivar can contribute to animal production and sustainable agriculture by reducing or eliminating the need for P or phytase supplementation and by reducing the amount of P released into the environment from animal production facilities. C1 [Bregitzer, Phil; Hu, Gongshe; Raboy, Victor] ARS, USDA, Natl Small Grains Germplasm Res Ctr, 1691 S 2700 W, Aberdeen, ID 83210 USA. [Marshall, Juliet M.] Univ Idaho, Res & Extens Ctr, 1693 S 2700 W, Aberdeen, ID 83210 USA. RP Bregitzer, P (reprint author), ARS, USDA, Natl Small Grains Germplasm Res Ctr, 1691 S 2700 W, Aberdeen, ID 83210 USA. EM phil.bregitzer@ars.usda.gov FU USDA Agricultural Research Service (ARS) [2050-21000-031-00 D] FX This research was funded by the USDA Agricultural Research Service (ARS), project 2050-21000-031-00 D. The USDA-ARS is an equal opportunity employer. NR 17 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 105 EP 108 DI 10.3198/jpr2015.09.0050crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400001 ER PT J AU Kelly, JD Varner, GV Hooper, S Cichy, KA Wright, EM AF Kelly, James D. Varner, Gregory V. Hooper, Sharon Cichy, Karen A. Wright, Evan M. TI Registration of 'Samurai' Otebo Bean SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID PHASEOLUS-VULGARIS L.; RESISTANT STARCH AB 'Samurai' otebo bean (Phaseolus vulgaris L.) (Reg. No. CV-312, PI 676022), developed by Michigan State University AgBioResearch, was released in 2015 as an upright, full-season cultivar with virus (caused by Bean common mosaic virus) resistance and high yield potential. Samurai was developed using pedigree breeding method to the F-6 generation followed by pure line selection for disease, agronomic, and quality traits. In 3 yr of field trials, Samurai yielded 2828 kg ha(-1), flowered in 45 d, and matured in 100 d on average. Plants averaged 52 cm in height, with lodging resistance score of 1.6 and seed weight of 28.1 g 100 seed(-1). Samurai combines high yield potential with erect architecture with full-season maturity in an otebo seed type. Samurai has resistance to lodging and high pod placement within plant structure, making it suitable for direct harvest under narrow-row production systems. Current otebo cultivars are short-statured plants with determinate growth habit that require traditional pulling equipment due to the excessive seed loss that would result from direct harvest. Samurai is susceptible to race 73 of anthracnose [caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.-Scrib], susceptible to rust race 22: 2 [caused by Uromyces appendiculatus (Pers.:Pers.) Unger], and hypersensitive resistant to strain NL 3 of Bean common mosaic necrosis virus. Samurai otebo bean seed differs from other white-seeded navy and great northern cultivars in possessing low levels of resistant starch, which makes it better suited for paste rather than canned bean products that meet industry standards. C1 [Kelly, James D.; Hooper, Sharon; Wright, Evan M.] Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. [Varner, Gregory V.] Michigan Dry Bean Prod Res Advisory Board, 8439 N Blair Rd, Breckenridge, MI 48615 USA. [Cichy, Karen A.] ARS, USDA, E Lansing, MI 48824 USA. [Cichy, Karen A.] Michigan State Univ, Dept Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Kelly, JD (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA. EM kellyj@msu.edu FU Michigan State University AgBioResearch; Michigan Crop Improvement Association; Michigan Bean Commission FX The authors wish to acknowledge the field testing of Samurai by J. Osorno in North Dakota, J. Beaver in Puerto Rico through W2150 multistate project, and S. Willis in Ontario; with financial support from the Michigan State University AgBioResearch, Michigan Crop Improvement Association, and the Michigan Bean Commission. NR 19 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 109 EP 114 DI 10.3198/jpr2015.09.0051crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400002 ER PT J AU Afghan, S Shahzad, A Comstock, JC Zhao, DL Ali, A AF Afghan, Shahid Shahzad, Aamir Comstock, Jack C. Zhao, Duli Ali, Aamir TI Registration of 'CPSG-3481' Sugarcane SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'CPSG-3481' (Reg. No. CV-165, PI 676023) sugarcane (a complex hybrid of Saccharum spp.) cultivar was developed through cooperative research conducted by the Shakarganj Sugar Research Institute in Pakistan and the USDA-ARS Sugarcane Field Station in the United States and released to growers for loam soils in Pakistan in September 2012. CPSG-3481 was developed from a biparental cross (CP 81-1238 x CP 85-1308) made at Canal Point (CP), FL, USA, in December 1992, and selection process was made at the Shakarganj Sugar Research Institute, Punjab, Pakistan, in 2001-2011. CPSG-3481 was commercially released by the variety evaluation committee of sugarcane scientists from the public and private sectors in the sugarcane production region (Punjab, Pakistan) in 2012 because of its high cane and sucrose yields and high commercial recoverable sucrose on loam soils and its acceptable levels of resistance to red rot (caused by Colletotrichum falcatum Went), brown rust (caused by Puccinia melanocephala Syd. & P. Syd.), pokkah boeng (caused by Fusarium moniliforme J. Sheldon), Sugarcane mosaic virus, and smut (caused by Ustilago scitaminea Syd. & P. Syd.) in Pakistan. Based on results of nine harvests of three crops (plant cane, first ratoon, and second ratoon) in the final-stage replicated yield trials conducted at three locations on loam soils and compared with a reference cultivar HSF-240, CPSG-3481 had 19.4% higher (p < 0.030) cane yield, 19.7% higher (p < 0.001) commercial recoverable sucrose, 43.0% higher (p < 0.001) sucrose yield, and 19.4% greater (p < 0.001) economic index. C1 [Afghan, Shahid; Shahzad, Aamir] Shakarganj Sugar Res Inst, Jhang, Punjab, Pakistan. [Comstock, Jack C.; Zhao, Duli] ARS, USDA, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. [Ali, Aamir] Univ Sargodha, Dept Bot, Punjab, Pakistan. RP Afghan, S (reprint author), Shakarganj Sugar Res Inst, Jhang, Punjab, Pakistan.; Zhao, DL (reprint author), ARS, USDA, Sugarcane Field Stn, 12990 US Highway 441 N, Canal Point, FL 33438 USA. EM shahid.afghan@shakarganj.com.pk; duli.zhao@ars.usda.gov FU Shakarganj Limited; USDA-ARS [CPSG-3481] FX We are grateful to the management of Shakarganj Limited for their financial support and USDA-ARS for their technical and in kind support for supplying CP cane fuzz (true seed) to develop CPSG-3481. In particular, we wish to thank Mr. Altaf M. Saleem (Chairman Shakarganj Sugar Research Institute), Mr. Ali Altaf Saleem (Deputy Managing Director), and the USDA-ARS Sugarcane Field Station team for providing technical guidance and moral support that enabled our sugarcane crop improvement program a reality as 'CPSG-3481'. NR 32 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 124 EP 129 DI 10.3198/jpr2015.10.0065crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400005 ER PT J AU Mergoum, M Simsek, S Zhong, SB Acevedo, M Friesen, TL Alamri, MS Xu, S Liu, ZH AF Mergoum, Mohamed Simsek, Senay Zhong, Shaobin Acevedo, Maricelis Friesen, Timothy L. Alamri, Mohammed S. Xu, Steven Liu, Zhaohui TI 'Elgin-ND' Spring Wheat: A Newly Adapted Cultivar to the North-Central Plains of the United States with High Agronomic and Quality Performance SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID PYRENOPHORA-TRITICI-REPENTIS; FUSARIUM HEAD BLIGHT; TAN SPOT; REGISTRATION; RESISTANCE AB The spring wheat (Triticum aestivum L.) industry and growers usually value adapted wheat cultivars with high quality attributes, an essential criteria for maintaining wheat as a competitive commodity at the national and international levels. Therefore, the goal of the breeding program is to develop wheat cultivars that meet the above criteria using appropriate breeding tools. 'Elgin-ND' (CV-1116, PI 668099), a hard red spring wheat (HRSW) developed at North Dakota State University was released by the North Dakota Agricultural Experiment Station in 2013. Elgin-ND, tested as experimental line ND818, was released because it is adapted to the US spring wheat growing conditions. It combines high yield potential with high protein and end-use quality and has a good disease resistance package. This includes resistance to Fusarium head blight [caused by Fusarium graminearum Schwabe; teleomorph Gibberella zeae (Schwein.) Petch)]; leaf diseases including stem rust (caused by Puccinia graminis Pers.: Pers. f. sp. tritici Eriks. & E. Henn) and leaf rust (caused by Puccinia triticina Eriks.); tan spot [caused by Pyrenophora tritici-repentis (Died.) Drechs]; and bacterial leaf blight (caused by Xanthomonas translucens pv. undulosa). However, Elgin-ND relies heavily on the major Lr21 gene that confers resistance to leaf rust. Thus, it is susceptible to the new race that overcomes the Lr21 gene. The name Elgin-ND was chosen for ND818 after a small town in western North Dakota, where the cultivar is expected to be widely planted. C1 [Mergoum, Mohamed] Univ Georgia, Dept Crop & Soil Sci, 1109 Expt St, Griffin, GA 30223 USA. [Simsek, Senay] North Dakota State Univ, Plant Sci, 1250 Bolley Dr, Fargo, ND 58105 USA. [Zhong, Shaobin; Acevedo, Maricelis; Liu, Zhaohui] North Dakota State Univ, Plant Pathol, 306 Walster Hall, Fargo, ND 58105 USA. [Friesen, Timothy L.] ARS, USDA, Red River Valley Agr Res Ctr, Cereal Crops Res Unit, 1307 N 18th St, Fargo, ND 58105 USA. [Alamri, Mohammed S.] King Saud Univ, Nutr Coll Food & Agr Sci, Riyadh, Saudi Arabia. [Xu, Steven] ARS, USDA, Cereal Crops Res Unit, 1307 18th St North, Fargo, ND 58102 USA. RP Mergoum, M (reprint author), Univ Georgia, Dept Crop & Soil Sci, 1109 Expt St, Griffin, GA 30223 USA. EM mmergoum@uga.edu NR 16 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 130 EP 134 DI 10.3198/jpr2015.07.0044crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400006 ER PT J AU Berg, JE Lamb, PF Miller, JH Wichman, DM Kephart, KD Stougaard, RN Pradhan, GP Nash, DL Grey, WE Gettel, D Gale, S Jin, Y Kolmer, JA Chen, X Bai, G Murray, TD Bruckner, PL AF Berg, J. E. Lamb, P. F. Miller, J. H. Wichman, D. M. Kephart, K. D. Stougaard, R. N. Pradhan, G. P. Nash, D. L. Grey, W. E. Gettel, D. Gale, S. Jin, Y. Kolmer, J. A. Chen, X. Bai, G. Murray, T. D. Bruckner, P. L. TI Registration of 'Northern' Hard Red Winter Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article AB 'Northern' (Reg. No. CV-1114, PI 676026) hard red winter wheat (Triticum aestivum L.) was developed and released by the Montana Agricultural Experiment Station in 2015. Northern was derived from a composite of two crosses, MT9982//MTW0072/NW97151 and MTW0047//MTW0072/NW97151. Northern was developed using a modified bulk breeding method and selected as an F-5:7 headrow. Northern was tested under the experimental number MT0978 in Montana yield trials from 2009 to 2015. Like predominant cultivar Yellowstone, Northern is a high-yielding, winter-hardy hard red winter wheat cultivar with medium to late maturity, medium to high grain protein, and acceptable milling and baking quality. Northern was released for its excellent performance in winter wheat production environments of north-central Montana, reduced plant height, and improved grain volume weight and resistance to stem rust (caused by Puccinia graminis Pers.: Pers. f. sp. tritici Eriks. & E. Henn.) relative to Yellowstone. C1 [Berg, J. E.; Nash, D. L.; Grey, W. E.; Bruckner, P. L.] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Lamb, P. F.] Northern Agr Res Ctr, 3710 Assinniboine Rd, Havre, MT 59501 USA. [Miller, J. H.] Western Triangle Agr Res Ctr, 9546 Old Shelby Rd, Conrad, MT 59425 USA. [Wichman, D. M.] Cent Agr Res Ctr, 52583 US Hwy 87, Moccasin, MT 59462 USA. [Kephart, K. D.] Southern Agr Res Ctr, 748 Railrd Hwy, Huntley, MT 59037 USA. [Stougaard, R. N.] Northwestern Agr Res Ctr, 4570 Montana 35, Kalispell, MT 59901 USA. [Pradhan, G. P.] Williston Res Extens Ctr, 14120 Hwy 2, Williston, ND 58801 USA. [Gettel, D.] Montana Agr Exptl Stn, Bozeman, MT 59717 USA. [Gale, S.; Jin, Y.; Kolmer, J. A.] ARS, USDA, Cereal Dis Lab, 1551 Lindig St, St Paul, MN 55108 USA. [Chen, X.] ARS, USDA, POB 646430, Pullman, WA 99164 USA. [Bai, G.] ARS, USDA, Hard Winter Wheat Genet Res Unit, 4008 Throckmorton Hall, Manhattan, KS 66506 USA. [Murray, T. D.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP Bruckner, PL (reprint author), Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. EM bruckner@montana.edu FU Montana Agricultural Experiment Station; Montana Wheat and Barley Committee; Hatch project [MONB00298] FX Northern was developed with financial support of the Montana Agricultural Experiment Station, the Montana Wheat and Barley Committee, and Hatch project MONB00298. The authors wish to acknowledge the technical assistance of Ron Ramsfield, Ron Larson, Jackie Kennedy, and Harvey Teslaa. NR 8 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 135 EP 138 DI 10.3198/jpr2015.10.0062crc PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400007 ER PT J AU Beaver, JS Prophete, E Demosthene, G Porch, TG AF Beaver, James S. Prophete, Emmanuel Demosthene, Gasner Porch, Timothy G. TI Registration of PR1146-138 Yellow Bean Germplasm Line SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID BACTERIAL-BLIGHT RESISTANCE; POTYVIRUS RESISTANCE; MOSAIC-VIRUS; I-GENE; COMMON; MARKER; IDENTIFICATION; QTL AB The yellow bean (Phaseolus vulgaris L.) is an important market class in Haiti. There have been, however, no previous attempts to genetically improve this seed type for the Caribbean. Landrace varieties of yellow beans in Haiti are susceptible to Bean golden yellow mosaic virus (BGYMV) and Bean common mosaic virus (BCMV), which are important dry bean diseases in the region. In addition, leafhopper (Empoasca kraemeri Ross and Moore) can cause significant yield loss, especially when common beans are planted during periods of low precipitation. The development of dry bean cultivars with enhanced levels of resistance to diseases and pests is an important goal of the University of Puerto Rico (UPR) and the National Seed Service in Haiti. PR1146-138 (Reg. No. GP-298, PI 675225) is a multiple virus-and pest-resistant bean germplasm line adapted to the humid tropics that was developed and released cooperatively in 2015 by the UPR Agricultural Experiment Station, the USDA-ARS, and the National Seed Service of the Ministry of Agriculture, Natural Resources and Rural Development of the Republic of Haiti. PR1146-138 has a yellow seed type with a hue angle of 86.30 and a chroma of 34.68. PR1146-138 possesses the I locus that confers resistance to BCMV and the bgm-1 gene for resistance to BGYMV. PR1146-138 produced a mean seed yield of 2095 kg ha(-1) when tested in Puerto Rico and Haiti in seven environments over a 3-yr period. It has a determinate growth habit and reached harvest maturity from 65 to 70 d after planting. This line should serve as a useful source of resistance to BGYMV, BCMV, and leafhoppers in the improvement of Andean beans for the tropical lowlands. C1 [Beaver, James S.] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR 00681 USA. [Prophete, Emmanuel; Demosthene, Gasner] Nat Resources & Rural Dev Republ Haiti, Natl Seed Serv, Minist Agr, Natl Rd 1, Port Au Prince, Haiti. [Porch, Timothy G.] ARS, USDA, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. RP Beaver, JS (reprint author), Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR 00681 USA. EM james.beaver@upr.edu FU Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the United States Agency for International Development (USAID) as part of Feed the Future; US government's global hunger and food security initiative [EDH-A-00-07-00005-00] FX Support provided by the Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the United States Agency for International Development (USAID) as part of Feed the Future, the US government's global hunger and food security initiative, under the terms of Cooperative Agreement No. EDH-A-00-07-00005-00. The opinions expressed herein are those of the Legume Innovation Lab and do not necessarily reflect the views of USAID or the US government. NR 24 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 145 EP 148 DI 10.3198/jpr2015.09.0058crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400009 ER PT J AU Kusolwa, PM Myers, JR Porch, TG Trukhina, Y Gonzalez-Velez, A Beaver, JS AF Kusolwa, Paul M. Myers, James R. Porch, Timothy G. Trukhina, Yulia Gonzalez-Velez, Abiezer Beaver, James S. TI Registration of AO-1012-29-3-3A Red Kidney Bean Germplasm Line with Bean Weevil, BCMV, and BCMNV Resistance SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID PHASEOLUS-VULGARIS L; SEED PROTEIN; COMMON; ARCELIN AB The bean weevil (Acanthoscelides obtectus Say) is an aggressive post-harvest pest of the common bean (Phaseolus vulgaris L.). Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV) are important seedborne diseases of dry bean in the Americas and Africa. The development of bean lines that combine resistance to BCMV, BCMNV, and bean weevils can help to reduce yield loss during the growing season and avoid seed damage during storage. AO-1012-29-3-3A, (Reg. No. GP-299, PI 675563) is a multiple virus and bean weevil-resistant bean germplasm line adapted to the humid tropics; it was developed and released cooperatively in 2015 by Sokoine University of Agriculture, Oregon State University, the USDA-ARS, and the University of Puerto Rico. AO-1012-29-3-3A has a determinate growth habit and a dark red kidney seed type similar to the cultivar Rojo, which is commercially acceptable in Tanzania. AO-1012-29-3-3A possesses the I and bc-1(2) genes that confer resistance to BCMV and BCMNV. AO-1012-29-3-3A produced a mean seed yield similar to the checks USLK-1 and 'Badillo' in three trials planted in Puerto Rico. Seed of AO-1012-29-3-3A had greater levels of threonine, proline, analine, valine, lysine, methionine, and crude protein compared with the check cultivar Badillo. AO-1012-29-3-3A should serve as a useful source of resistance to the bean weevil, BCMV, and some pathogroups of BCMNV. C1 [Kusolwa, Paul M.] Sokoine Univ Agr, POB 3005, Morogoro, Tanzania. [Myers, James R.] Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. [Porch, Timothy G.; Trukhina, Yulia] ARS, USDA, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. [Gonzalez-Velez, Abiezer; Beaver, James S.] Univ Puerto Rico, Dept Crop & Agroenvironm Sci, Mayaguez, PR 00681 USA. RP Beaver, JS (reprint author), Univ Puerto Rico, Dept Crop & Agroenvironm Sci, Mayaguez, PR 00681 USA. EM james.beaver@upr.edu FU Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the United States Agency for International Development (USAID) as part of Feed the Future; US Government's global hunger and food security initiative [EDH-A-00-07-00005-00] FX Support provided by the Feed the Future Innovation Lab for Collaborative Research on Grain Legumes by the United States Agency for International Development (USAID) as part of Feed the Future, the US Government's global hunger and food security initiative, under the terms of Cooperative Agreement No. EDH-A-00-07-00005-00. The opinions expressed herein are those of the Legume Innovation Lab and do not necessarily reflect the views of USAID or the US Government. We also thank Mr. Joel Davis, Oregon State University for conducting the SDS-PAGE. NR 25 TC 1 Z9 1 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 149 EP 153 DI 10.3198/jpr2015.10.0064crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400010 ER PT J AU Ulloa, M Hutmacher, RB Percy, R Wright, SD Burke, J AF Ulloa, Mauricio Hutmacher, Robert B. Percy, Richard Wright, Steven D. Burke, John TI Registration of Five Pima Cotton Germplasm Lines (Pima SJ-FR05-Pima SJ-FR09) with Improved Resistance to Fusarium Wilt Race 4 and Good Lint Yield and Fiber Quality SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID F-SP VASINFECTUM; CALIFORNIA AB Five pima (Gossypium barbadense L.) cotton germplasm lines-Pima SJ-FR05 (Reg. No. GP-1000, PI 675557), Pima SJ-FR06 (Reg. No. GP-1001, PI 675558), Pima SJ-FR07 (Reg. No. GP-1002, PI 675559), Pima SJ-FR08 (Reg. No. GP-1003, PI 675560), and Pima SJ-FR09 (Reg. No. GP-1004, PI 675561)-were developed by the USDA-ARS and jointly released with the University of California in 2015. The primary purpose for these releases is to provide germplasm with good levels of resistance to Fusarium wilt, caused by Fusarium oxysporum f. sp. vasinfectum (FOV) race 4, to cotton breeders. The Pima SJ-FR05-SJ-FR09 series originated from the cross of 'Pima S-6' and germplasm line 89590 in 1999 at the USDA-ARS in Maricopa, AZ. Pima S-6 possesses a major gene(s) for FOV race 4 resistance. Initially, the Pima SJ-FR05-SJ-FR09 lines were developed from the F-2 to F-6 from a single seed-plant descendant. In 2006, a recurrent selection was applied for each line in fields infested with FOV race 4 at two locations in California. In subsequent field and greenhouse tests, the Pima SJ-FR05-SJ-FR09 lines showed good resistance to FOV race 4 and good lint yield and fiber quality when compared to 'Phytogen 800', the San Joaquin Valley pima cotton standard. The Pima SJ-FR05-SJ-FR09 series will broaden the genetic base of resistance, which is critical to the success and future of the pima cotton industry. C1 [Ulloa, Mauricio; Burke, John] ARS, USDA, PA, CSRL,Plant Stress & Germplasm Dev Res Unit, 3810 4th St, Lubbock, TX 79415 USA. [Hutmacher, Robert B.] Univ Calif Davis, Dept Plant Sci, 17353 W Oakland Ave, Five Point, CA 93624 USA. [Percy, Richard] ARS, USDA, SPARC, Crop Germplasm Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. [Wright, Steven D.] Univ Calif, Cooperat Extens, 4437B S Laspina ST, Tulare, CA 93274 USA. RP Ulloa, M (reprint author), ARS, USDA, PA, CSRL,Plant Stress & Germplasm Dev Res Unit, 3810 4th St, Lubbock, TX 79415 USA. EM Mauricio.Ulloa@ars.usda.gov FU Cotton Foundation; Supima Association FX Special thanks for field and greenhouse project support provided by Mark Keeley, Gerardo Banuelos, Raul Delgado, Nick E. Clark, TariLee Frigulti, many other students from University of California County Cooperative Extension and Research and Extension Centers, and USDA-ARS. Access to field sites was possible with the permission and assistance of growers. Use of the greenhouse facilities of the University of California at the Kearney Research and Extension Center (Parlier, CA) and assistance of Fred Swanson and Laura van der Staay of that center is gratefully acknowledged. Farm equipment and help of staff from the University of California Shafter Research Center is also gratefully acknowledged. We also thank the Cotton Foundation and Supima Association for partial support of this study. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture or the University of California. The US Department of Agriculture and the University of California are equal opportunity providers and employers. NR 17 TC 0 Z9 0 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 154 EP 158 DI 10.3198/jpr2015.07.0043crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400011 ER PT J AU Vogel, KP Mitchell, RB Sarath, G AF Vogel, Kenneth P. Mitchell, Robert B. Sarath, Gautam TI Registration of NE Trailblazer C-1, NE Trailblazer C0, NE Trailblazer C2, NE Trailblazer C3, NE Trailblazer C4, and NE Trailblazer C5 Switchgrass Germplasms SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID DRY-MATTER DIGESTIBILITY; DIFFERING DIGESTIBILITY; SELECTION; POPULATIONS; GENOTYPES; INVITRO; IVDMD AB NE Trailblazer C-1 (GP-101, PI 672015), NE Trailblazer C0 (GP-100, PI 672014), NE Trailblazer C2 (GP-102, PI 672016), NE Trailblazer C3 (GP-103, PI 672017), NE Trailblazer C4 (GP-104, PI 672018), and NE Trailblazer C5 (GP-105, PI 672019) switchgrass (Panicum virgatum L.) germplasms were released by the USDA-ARS and the University of Nebraska-Lincoln on 10 Sept. 2014. These germplasms were developed by six generations of divergent breeding for in vitro dry matter digestibility (IVDMD). As a result of the multigenerations of recurrent breeding, the resulting populations differ significantly for IVDMD and for 25 other forage quality or biomass composition traits, including both acid detergent and Klason lignin. Plants in the high IVDMD populations also had altered anatomical structure. The lignin concentration of the stems was altered more than that of the leaves. The germplasm populations also differ significantly for winter survival. Altering plant composition by selection for IVDMD adversely affected the winter survival fitness of the resulting populations by unknown mechanisms. Plants from these germplasms can be used in genetics studies for determining the inheritance of multiple biomass composition traits and for identifying genes controlling specific biomass composition properties and winter survival of switchgrass and other perennial grasses. C1 [Vogel, Kenneth P.; Mitchell, Robert B.; Sarath, Gautam] Univ Nebraska, ARS, USDA, Grain Forage & Bioenergy Res Unit,Dept Agron & Ho, 251 Filley Hall, Lincoln, NE 68583 USA. RP Vogel, KP (reprint author), Univ Nebraska, ARS, USDA, Grain Forage & Bioenergy Res Unit,Dept Agron & Ho, 251 Filley Hall, Lincoln, NE 68583 USA. EM vogelkp61@neb.rr.com NR 17 TC 1 Z9 1 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 159 EP 165 DI 10.3198/jpr2015.11.0070crg PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400012 ER PT J AU Pinson, SRM Wang, YG Tabien, RE AF Pinson, Shannon R. M. Wang, Yueguang Tabien, Rodante E. TI Registration of TIL:383.13, TIL:625 and TIL:634, Three Long Grain Tropical Japonica Rice Germplasm Lines Containing Novel Indica Alleles that Increase Tiller Production and Grain Yield SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID SHEATH BLIGHT RESISTANCE; FIELD-RESISTANCE; LOCI; QTLS; LEMONT; GENOTYPES; TRAITS AB Three rice (Oryza sativa L.) germplasm lines designated TIL:383.13 (Reg. No. GP-144, GSOR 100605), TIL: 625 (Reg. No. GP-145, GSOR 100698), and TIL: 634 (Reg. No. GP-146, GSOR 100702), contain a total of seven alleles of indica rice origin associated with earlier development and increased numbers of tillers during early plant development stages in an elite US tropical japonica rice genetic background having long grain shape and intermediate amylose content and gelatinization temperature. The three high-tillering introgression lines also exhibit increased panicle number and grain yield and were jointly released in 2016 by the USDA-ARS and Texas A&M Agrilife Research. The novel alleles for enhanced tiller production originated from 'TeQing', an indica rice cultivar from China, and were transferred through a series of backcrossing into lines having a predominantly 'Lemont' genetic background. TeQing is well known in the United States for both its high yield and its disease resistance but is not suitable for commercial production due to lodging and poor grain quality. A combination of molecular and phenotypic data was used to identify these three improved germplasm lines from among 123 TeQing-into-Lemont backcross introgression lines. A combination of phenotypic and molecular data was used to select three lines from among 123 TeQing-into-Lemont backcross introgression lines. C1 [Pinson, Shannon R. M.] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130 East, Stuttgart, AR 72160 USA. [Wang, Yueguang] Univ Idaho, Moscow, ID 83843 USA. [Tabien, Rodante E.] Texas A&M Agrilife Res, Beaumont, TX USA. RP Pinson, SRM (reprint author), ARS, USDA, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130 East, Stuttgart, AR 72160 USA. EM shannon.pinson@ars.usda.gov FU Texas Rice Research Foundation FX This research was supported in part by a grant from the Texas Rice Research Foundation. The authors acknowledge the technical skills and support provided by Mr. Eric Grunden, whose expertise and dedication in monitoring detailed aspects of plant growth in field plots were essential to this germplasm selection. Grain chemistry data was provided by the USDA-ARS Cereal Chemistry Laboratory at the Dale Bumpers National Rice Research Center, Stuttgart, AR. NR 25 TC 0 Z9 0 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 171 EP 176 DI 10.3198/jpr2014.09.0069crg PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400014 ER PT J AU Qi, LL Seiler, GJ AF Qi, L. L. Seiler, G. J. TI Registration of an Oilseed Sunflower Germplasm HA-DM1 Resistant to Sunflower Downy Mildew SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID HELIANTHUS-ANNUUS L.; CULTIVATED SUNFLOWER; MALE-STERILITY; GENE AB HA-DM1 (Reg. No. GP-335, PI 674793) sunflower (Helianthus annuus L.) germplasm was developed and released cooperatively by the USDA-ARS, Sunflower and Plant Biology Research Unit and the North Dakota Agricultural Experiment Station in 2015. HA-DM1 is a BC2F4-derived oilseed maintainer line from the cross HA 89*2/NMS HA 89/Helianthus argophyllus PI 494573, selected for resistance to downy mildew (DM) [caused by Plasmopara halstedii (Farl.) Berl. et de Toni], a destructive disease in sunflower. The DM resistance in HA-DM1 originated from wild H. argophyllus accession PI 494573 and is conditioned by a single dominant gene designated Pl(18).Pl(18) has been mapped to linkage group 2 of the sunflower genome and is independent of all known identified DM resistance genes in sunflower. HA-DM1 has resistance to all P. halstedii races identified in the United States to date, providing a potential new source for resistance to emerging virulent races of P. halstedii in sunflower in North America and around the world. C1 [Qi, L. L.; Seiler, G. J.] ARS, USDA, Northern Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. RP Qi, LL (reprint author), ARS, USDA, Northern Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM lili.qi@ars.usda.gov FU USDA-ARS CRIS Project [5442-21000-039-00D] FX We thank Angelia Hogness and Marjorie Olson for technical assistance. This project was supported by the USDA-ARS CRIS Project No. 5442-21000-039-00D. Mention of trade names or commercial products in this report is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 22 TC 1 Z9 1 U1 1 U2 1 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 195 EP 199 DI 10.3198/jpr2015.12.0072crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400017 ER PT J AU Burow, GB Burke, JJ Franks, CD Xin, Z Pederson, GA AF Burow, G. B. Burke, J. J. Franks, C. D. Xin, Z. Pederson, G. A. TI Registration of RTx430/Gaigaoliang Sorghum [Sorghum bicolor (L.) Moench] Recombinant Inbred Line Mapping Population SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID SEASON COLD TOLERANCE; SEEDLING VIGOR; GRAIN-SORGHUM; SELECTION AB Sorghum [Sorghum bicolor (L.) Moench.] recombinant inbred line (RIL) mapping population RTx430 x Gaigaoliang (Reg. No. MP-2, NSL 515993 MAP) was developed at the Plant Stress and Germplasm Development Unit, Cropping Systems Research Laboratory, USDA-ARS, Lubbock, TX, and released in 2011 via the Germplasm Resources Information Network (GRIN). This population was developed to map early-season field germinability, seedling vigor, and other important agronomic traits in sorghum and to serve as a long-term genetic resource. The population is composed of 171 F-7 RILs developed by single seed descent method and was evaluated at two locations for field emergence and seedling vigor at Lubbock and New Deal, TX, under early-season planting (1 April) and under controlled conditions at 12 degrees C. The genotype data for this population consist of 141 simple sequence repeat markers and 2922 single nucleotide polymorphism obtained through genotype-by-sequencing technology. Ten quantitative trait loci were detected for early-season field germination and were used to identify early-season cold-tolerant recombinant inbreds with nonpigmented testa that can be used as pollinators in breeding programs. C1 [Burow, G. B.; Burke, J. J.; Xin, Z.] ARS, USDA, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [Franks, C. D.] Pioneer HiBred Int Inc, Plainview, TX 79072 USA. [Pederson, G. A.] ARS, USDA, Plant Genet Resource & Conservat Unit, Griffin, GA 30223 USA. RP Burow, GB (reprint author), ARS, USDA, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA. EM gloria.burow@ars.usda.gov FU US Sorghum Checkoff Program (USCP) Project through USCP project [R0010-11, CI005-12] FX The authors would like to acknowledge Dr. Patrick Brown, Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, for generously sharing the genotype-by-sequencing data, (SNP genotype) for the RIL population. The authors thank Ms. Halee Hughes and Mr. Charlie Woodfin for their technical support and student workers who have worked on various projects conducted on this population. Financial support from the US Sorghum Checkoff Program (USCP) Project through USCP project R0010-11 and CI005-12 are gratefully acknowledged. NR 18 TC 0 Z9 0 U1 3 U2 3 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2016 VL 10 IS 2 BP 206 EP 209 DI 10.3198/jpr2015.03.0017crmp PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DY4AM UT WOS:000385041400019 ER PT J AU Alexander, KA Sanderson, CE Larsen, MH Robbe-Austerman, S Williams, MC Palmer, MV AF Alexander, Kathleen A. Sanderson, Claire E. Larsen, Michelle H. Robbe-Austerman, Suelee Williams, Mark C. Palmer, Mitchell V. TI Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo) (vol 7, e00281-16, 2016) SO MBIO LA English DT Correction C1 [Alexander, Kathleen A.; Sanderson, Claire E.] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Alexander, Kathleen A.; Sanderson, Claire E.] Ctr Conservat African Resources, Ctr Conservat African Resources Anim Communities, CARACAL, Kasane, Botswana. [Larsen, Michelle H.] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA. [Robbe-Austerman, Suelee] Natl Vet Serv Labs, Diagnost Bacteriol Lab, Ames, IA USA. [Williams, Mark C.] Univ Pretoria, Onderstepoort, South Africa. [Palmer, Mitchell V.] Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, Ames, IA USA. RP Alexander, KA (reprint author), Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA.; Alexander, KA (reprint author), Ctr Conservat African Resources, Ctr Conservat African Resources Anim Communities, CARACAL, Kasane, Botswana. EM kathyalx@vt.edu NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD MAY-JUN PY 2016 VL 7 IS 3 PG 1 WC Microbiology SC Microbiology GA DW1YP UT WOS:000383440300010 ER PT J AU Alexander, KA Sanderson, CE Larsen, MH Robbe-Austerman, S Williams, MC Palmer, MV AF Alexander, Kathleen A. Sanderson, Claire E. Larsen, Michelle H. Robbe-Austerman, Suelee Williams, Mark C. Palmer, Mitchell V. TI Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo) SO MBIO LA English DT Article ID MYCOBACTERIUM-BOVIS BCG; BADGER MELES-MELES; SCENT MARKING; INFECTIOUS-DISEASES; ESCHERICHIA-COLI; AFRICAN WILDLIFE; UNITED-STATES; EPIDEMIOLOGY; COMPLEX; DEER AB An emerging Mycobacterium tuberculosis complex (MTC) pathogen, M. mungi, infects wild banded mongooses (Mungos mungo) in Northern Botswana, causing significant mortality. This MTC pathogen did not appear to be transmitted through a primary aerosol or oral route. We utilized histopathology, spoligotyping, mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR), quantitative PCR (qPCR), and molecular markers (regions of difference [RDs] from various MTC members, including region of difference 1 [RD1] from M. bovis BCG [RD1(BCG)], M. microti [RD1(mic)], and M. pinnipedii [RD1(seal)], genes Rv1510 [RD4], Rv1970 [RD7], Rv3877/8 [RD1], and Rv3120 [RD12], insertion element IS1561, the 16S RNA gene, and gene Rv0577 [cfp32]), including the newly characterized mongoose-specific deletion in RD1 (RD1(mon)), in order to demonstrate the presence of M. mungi DNA in infected mongooses and investigate pathogen invasion and exposure mechanisms. M. mungi DNA was identified in 29% of nasal planum samples (n = 52), 56% of nasal rinses and swabs (n = 9), 53% of oral swabs (n = 19), 22% of urine samples (n = 23), 33% of anal gland tissue (n = 18), and 39% of anal gland secretions (n = 44). The occurrence of extremely low cycle threshold values obtained with qPCR in anal gland and nasal planum samples indicates that high levels of M. mungi can be found in these tissue types. Histological data were consistent with these results, suggesting that pathogen invasion occurs through breaks in the nasal planum and/or skin of the mongoose host, which are in frequent contact with anal gland secretions and urine during olfactory communication behavior. Lesions in the lung, when present, occurred only with disseminated disease. No environmental sources of M. mungi DNA could be found. We report primary environmental transmission of an MTC pathogen that occurs in association with social communication behavior. IMPORTANCE Organisms causing infectious disease evolve modes of transmission that exploit environmental and host conditions favoring pathogen spread and persistence. We report a novel mode of environmental infectious disease transmission that occurs in association with olfactory secretions (e.g., urine and anal gland secretions), allowing pathogen exposure to occur within and between social groups through intricate social communication behaviors of the banded mongoose host. The presence of M. mungi in these environmentally deposited secretions would effectively circumvent natural social barriers (e.g., territoriality), facilitating between-group pathogen transmission in the absence of direct physical contact, a rare occurrence in this highly territorial species. This work identifies an important potential mechanism of pathogen transmission of epidemiological significance in social species. We also provide evidence of a novel mechanism of pathogen transmission for the MTC complex, where pathogen movement in the environment and host exposure dynamics are driven by social behavior. C1 [Alexander, Kathleen A.; Sanderson, Claire E.] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Alexander, Kathleen A.; Sanderson, Claire E.] Ctr Conservat African Resources Anim Communities, CARACAL, Kasane, Botswana. [Larsen, Michelle H.] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA. [Robbe-Austerman, Suelee] Natl Vet Serv Labs, Diagnost Bacteriol Lab, Ames, IA USA. [Williams, Mark C.] Univ Pretoria, Onderstepoort, South Africa. [Palmer, Mitchell V.] Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, Ames, IA USA. RP Alexander, KA (reprint author), Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA.; Alexander, KA (reprint author), Ctr Conservat African Resources Anim Communities, CARACAL, Kasane, Botswana. EM kathyalx@vt.edu FU Morris Animal Foundation [D14ZO-083]; National Science Foundation as part of the joint NSF-NIH-USDA Ecology and Evolution of Infectious Diseases program [1518663]; National Institute of General Medical Sciences of the National Institutes of Health [U01GM110748] FX This work was supported in part by Morris Animal Foundation grant number D14ZO-083 and by National Science Foundation grant number 1518663 as part of the joint NSF-NIH-USDA Ecology and Evolution of Infectious Diseases program. K. A. Alexander was also supported in part through the National Institute of General Medical Sciences of the National Institutes of Health under award number U01GM110748. The funders had no role in study design, data collection and analysis, or the decision to submit the work for publication. NR 60 TC 5 Z9 5 U1 3 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD MAY-JUN PY 2016 VL 7 IS 3 AR e00281-16 DI 10.1128/mBio.00281-16 PG 13 WC Microbiology SC Microbiology GA DW1YP UT WOS:000383440300009 ER PT J AU Alexander, KA Sanderson, CE Larsen, MH Robbe-Austerman, S Williams, MC Palmer, MV AF Alexander, Kathleen A. Sanderson, Claire E. Larsen, Michelle H. Robbe-Austerman, Suelee Williams, Mark C. Palmer, Mitchell V. TI Emerging Tuberculosis Pathogen Hijacks Social Communication Behavior in the Group-Living Banded Mongoose (Mungos mungo) (vol 7, 2016) SO MBIO LA English DT Correction C1 [Alexander, Kathleen A.; Sanderson, Claire E.] Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA. [Alexander, Kathleen A.; Sanderson, Claire E.] CARACAL, Ctr Conservat African Resources Anim Communities, Ctr Conservat African Resources, Kasane, Botswana. [Larsen, Michelle H.] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 USA. [Robbe-Austerman, Suelee] Natl Vet Serv Labs, Diagnost Bacteriol Lab, Ames, IA USA. [Williams, Mark C.] Univ Pretoria, Onderstepoort, South Africa. [Palmer, Mitchell V.] Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, Ames, IA USA. RP Alexander, KA (reprint author), Virginia Tech, Dept Fish & Wildlife Conservat, Blacksburg, VA 24061 USA.; Alexander, KA (reprint author), CARACAL, Ctr Conservat African Resources Anim Communities, Ctr Conservat African Resources, Kasane, Botswana. EM kathyalx@vt.edu NR 1 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 2150-7511 J9 MBIO JI mBio PD MAY-JUN PY 2016 VL 7 IS 3 AR e00921-16 DI 10.1128/mBio.00921-16 PG 1 WC Microbiology SC Microbiology GA DW1YP UT WOS:000383440300076 ER PT J AU Nichols, NN Saha, BC AF Nichols, Nancy N. Saha, Badal C. TI Production of Xylitol by a Coniochaeta ligniaria Strain Tolerant of Inhibitors and Defective in Growth on Xylose SO BIOTECHNOLOGY PROGRESS LA English DT Article DE biomass; fermentation inhibitors; xylitol ID RECOMBINANT SACCHAROMYCES-CEREVISIAE; PICHIA-STIPITIS; BIOLOGICAL DETOXIFICATION; LIGNOCELLULOSIC BIOMASS; BIOETHANOL PRODUCTION; ETHANOL-PRODUCTION; WHEAT-STRAW; FERMENTATION; HYDROLYSATE; GENES AB In conversion of biomass to fuels or chemicals, inhibitory compounds arising from physical-chemical pretreatment of the feedstock can interfere with fermentation of the sugars to product. Fungal strain Coniochaeta ligniaria NRRL30616 metabolizes the furan aldehydes furfural and 5-hydroxymethylfurfural, as well as a number of aromatic and aliphatic acids and aldehydes. Use of NRRL30616 to condition biomass sugars by metabolizing the inhibitors improves their fermentability. Wild-type C. ligniaria has the ability to grow on xylose as sole source of carbon and energy, with no accumulation of xylitol. Mutants of C. ligniaria unable to grow on xylose were constructed. Xylose reductase and xylitol dehydrogenase activities were reduced by approximately two thirds in mutant C8100. The mutant retained ability to metabolize inhibitors in biomass hydrolysates. Although C. ligniaria C8100 did not grow on xylose, the strain converted a portion of xylose to xylitol, producing 0.59 g xylitol/g xylose in rich medium and 0.48 g xylitol/g xylose in corn stover dilute acid hydrolysate. (C) 2016 American Institute of Chemical Engineers Biotechnol. C1 [Nichols, Nancy N.; Saha, Badal C.] USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Nichols, NN (reprint author), USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM nancy.nichols@ars.usda.gov NR 41 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 8756-7938 EI 1520-6033 J9 BIOTECHNOL PROGR JI Biotechnol. Prog. PD MAY-JUN PY 2016 VL 32 IS 3 BP 606 EP 612 DI 10.1002/btpr.2259 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DW1GW UT WOS:000383392200008 PM 26950770 ER PT J AU Spooner, DM AF Spooner, David M. TI Species delimitations in plants: lessons learned from potato taxonomy by a practicing taxonomist SO JOURNAL OF SYSTEMATICS AND EVOLUTION LA English DT Review DE phylogeny; potato; Solanum section Petota; species ID SOLANUM SECT PETOTA; BREVICAULE COMPLEX SOLANACEAE; RESTRICTION SITE VARIATION; CHLOROPLAST DNA; WILD POTATOES; CULTIVATED POTATO; COLLECTING EXPEDITION; SERIES RELATIONSHIPS; GERMPLASM RESOURCES; MOLECULAR-DATA AB Solanum section Petota has been the subject of intensive taxonomic work since the description of the cultivated potato in 1753. In total, there are 494 epithets for wild taxa and 626 epithets for cultivated taxa. Different taxonomists applied various taxonomic philosophies and species concepts to the section. Hypotheses of the number of species and their interrelationships have differed greatly among authors. A taxonomic treatment of section Petota by Jack Hawkes in 1990 recognized 228 wild species and seven cultivated species, divided into 21 taxonomic series. In 2014 Spooner and collaborators more than halved this number to 107 wild species and four cultivated species, partitioned into four clades; not using series. The purpose of this paper is to provide a retrospective of the methods and philosophies that have resulted in this drastic decrease in the number of species and their infrasectional classification. C1 [Spooner, David M.] Univ Wisconsin, ARS, USDA, Vegetable Crops Res Unit,Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. RP Spooner, DM (reprint author), Univ Wisconsin, ARS, USDA, Vegetable Crops Res Unit,Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM david.spooner@ars.usda.gov NR 91 TC 2 Z9 2 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1674-4918 EI 1759-6831 J9 J SYST EVOL JI J. Syst. Evol. PD MAY PY 2016 VL 54 IS 3 BP 191 EP 203 DI 10.1111/jse.12203 PG 13 WC Plant Sciences SC Plant Sciences GA DW6ON UT WOS:000383770900002 ER PT J AU Pires, CSS Pereira, FD Lopes, MTD Nocelli, RCF Malaspina, O Pettis, JS Teixeira, EW AF Soares Pires, Carmen Silvia Pereira, Fabie de Mello do Rego Lopes, Maria Teresa Ferreira Nocelli, Roberta Cornelio Malaspina, Osmar Pettis, Jeffery Stuart Teixeira, Erica Weinstein TI Weakness and collapse of bee colonies in Brazil: are there cases of CCD? SO PESQUISA AGROPECUARIA BRASILEIRA LA Portuguese DT Review DE Apis mellifera; Africanized honey bees; colony collapse disorder; bee nutrition; honey bee health; pollination ID AFRICANIZED HONEY-BEES; APIS-MELLIFERA L.; SCAPTOTRIGONA-POSTICA LATREILLE; MELIPONA-SCUTELLARIS LATREILLE; NOSEMA-CERANAE; HYMENOPTERA APIDAE; NEONICOTINOID PESTICIDE; TOXICITY; NUTRITION; LOSSES AB In the last decades, the decline of wild bee populations and the collapse of Apis mellifera colonies have concerned researchers and beekeepers. The objective of this review was to compile some of the most relevant studies related to the possible causes of these problems such as nutrition, management, pathogens, parasites, and the effects of pesticides, besides presenting a brief history of the colony collapse disorder syndrome (CCD) in the United States and Europe. The studies developed in Brazil were presented in more detail, mainly those on pesticides used in agriculture and on pathogens and parasites that attack the colonies of Africanized A. mellifera. Cases of weakening, decline, and collapse have been recorded in Brazil, mainly in the states of Sao Paulo and Santa Catarina, which add up to great losses. Results of the analyzed occurrences indicated that the recorded mass mortalities were not associated with pathogens or parasites. Only two cases with characteristics similar to those described for CCD were recorded, but without defined causes. In order to know the causes of the weakening and collapses of colonies, the implementation of official programs is urgent for the systematic survey of bee health, associated with research focusing on assessments of possible impacts of habitat fragmentation and agricultural practices on bee communities. C1 [Soares Pires, Carmen Silvia] Embrapa Recursos Genet & Biotecnol, Parque Estacao Biol,Ave W5 Norte Final, BR-70770917 Brasilia, DF, Brazil. [Pereira, Fabie de Mello; do Rego Lopes, Maria Teresa] Embrapa Meio Norte, Ave Duque Caxias,5-650,Caixa Postal 001, BR-64006220 Teresina, PI, Brazil. [Ferreira Nocelli, Roberta Cornelio] Univ Fed Sao Carlos, Dept Ciencias Nat Matemat & Educ, Rodovia Anhanguera,Km 174, BR-13600970 Araras, SP, Brazil. [Malaspina, Osmar] Univ Estadual Paulista, Ctr Estudos Insetos Socials, Dept Biol, Inst Biociencias, Ave 24,1-515, BR-13506900 Rio Claro, SP, Brazil. [Pettis, Jeffery Stuart] ARS, USDA, Bee Res Lab, Bldg 306 BARC E,10300 Baltimore Ave, Beltsville, MD 20705 USA. [Teixeira, Erica Weinstein] SAA SP, Agencia Paulista Tecnol Agronegocios, Ave Prof Manoel Cesar Ribeiro,1-920, BR-12400970 Pindamonhangaba, SP, Brazil. RP Pires, CSS (reprint author), Embrapa Recursos Genet & Biotecnol, Parque Estacao Biol,Ave W5 Norte Final, BR-70770917 Brasilia, DF, Brazil. EM carmen.pires@embrapa.br; fabia.pereira@embrapa.br; maria-teresa.lopes@embrapa.br; roberta@cca.ufscar.br; malaspin@rc.unesp.br; Jeff.Pettis@ars.usda.gov; erica@apta.sp.gov.br NR 117 TC 0 Z9 0 U1 55 U2 55 PU EMPRESA BRASIL PESQ AGROPEC PI BRASILIA DF PA EMBRAPA INFORMACAO TECNOLOGICA, PESQUISA AGROPECUARIA BRASILEIRA - PAB, CAIXA POSTAL 040315, 70770-901 BRASILIA DF, BRAZIL SN 0100-204X EI 1678-3921 J9 PESQUI AGROPECU BRAS JI Pesqui. Agropecu. Bras. PD MAY PY 2016 VL 51 IS 5 BP 422 EP 442 DI 10.1590/S0100-204X2016000500003 PG 21 WC Agriculture, Multidisciplinary SC Agriculture GA DV7KK UT WOS:000383114600004 ER PT J AU Beniston, JW Lal, R Mercer, KL AF Beniston, Joshua W. Lal, Rattan Mercer, Kristin L. TI ASSESSING AND MANAGING SOIL QUALITY FOR URBAN AGRICULTURE IN A DEGRADED VACANT LOT SOIL SO LAND DEGRADATION & DEVELOPMENT LA English DT Article DE urban agriculture; soil quality; urban soil; compost; Soil Management Assessment Framework; vacant land; shrinking cities; soil compaction ID ORGANIC-MATTER; MANAGEMENT; RESTORATION; COMPACTION; COMPOST; CARBON AB Following the decline of industrial manufacturing, many US cities have experienced severe population reductions that have resulted in large areas of vacant land. Urban agriculture has emerged as a desirable land use for these spaces, but degraded soils are common. Therefore, we measured soil and plant responses to amendments and management in urban lots where vacant houses had recently been demolished in Youngstown, OH, USA. Soil degradation was observed following demolition activities in the form of compaction (bulk density of 1.5-1.8 Mg m(-3)) and low soil microbial biomass C (21 mg C kg(-1) soil). Our split-plot experiment measured the effects of organic matter (OM) amendments produced from yard wastes and the use of raised beds on soil properties and vegetable crop yields. Two years after their application, OM amendments resulted in significant improvement to a number of soil physical, chemical, and biological properties. Vegetable crop yields were improved by OM amendments in 2011 and by both OM amendments and the use of raised beds in 2012. A soil quality index, developed using factor analysis and the Soil Management Assessment Framework, produced values ranging from 0.60 to 0.85, which are comparable to those reported for rural agricultural soils. All results indicate that urban agriculture can be productive in vacant urban land and that amendments produced from urban yard wastes can improve soil quality at previously degraded sites and increase crop yields for urban agriculture. Copyright (C) 2015 John Wiley & Sons, Ltd. C1 [Beniston, Joshua W.; Lal, Rattan] Ohio State Univ, Carbon Management & Sequestrat Ctr, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Mercer, Kristin L.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. [Beniston, Joshua W.] New Mexico State Univ, USDA, ARS, Jornada Expt Range, Las Cruces, NM 88003 USA. RP Beniston, JW (reprint author), Ohio State Univ, Carbon Management & Sequestrat Ctr, Sch Environm & Nat Resources, Columbus, OH 43210 USA. EM beniston@nmsu.edu FU CERES Trust; USDA National Institute of Food and Agriculture [2012-67011-19668] FX The authors wish to thank Ian Beniston, Liberty Merrill, and the Youngstown Neighborhood Development Corporation for their collaboration with the construction and maintenance of the experimental site. We thank Claire Sutton and Ryan Hottle for assistance with lab and fieldwork, Diane Stott for help with SMAF, and Christopher Holloman for advice on data analysis. A portion of the funding for this study was provided by a graduate student grant from the CERES Trust and Competitive Grant No. 2012-67011-19668 from the USDA National Institute of Food and Agriculture. NR 59 TC 12 Z9 12 U1 23 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1085-3278 EI 1099-145X J9 LAND DEGRAD DEV JI Land Degrad. Dev. PD MAY PY 2016 VL 27 IS 4 BP 996 EP 1006 DI 10.1002/ldr.2342 PG 11 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA DS7LB UT WOS:000380963900013 ER PT J AU Rocha, BP Reis, MO Driemeier, D Cook, D Camargo, LM Riet-Correa, F Evencio-Neto, J Mendonca, FS AF Rocha, Brena P. Reis, Matheus O. Driemeier, David Cook, Daniel Camargo, Lazaro M. Riet-Correa, Franklin Evencio-Neto, Joaquim Mendonca, Fabio S. TI Liver biopsy as diagnostic method for poisoning by swainsonina-containing plants SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Poisonous plants; lysosomal storage disease; swainsonina; plant poisoning; liver biopsy ID LYSOSOMAL-STORAGE-DISEASE; CARNEA SUBSP FISTULOSA; NORTHEASTERN BRAZIL; IPOMOEA-VERBASCOIDEA; TURBINA-CORDATA; GOATS; CONVOLVULACEAE; MANNOSIDOSIS; SERICOPHYLLA; RIEDELII AB With the aim to investigate the use of hepatic biopsies for the diagnosis of poisoning by swainsonine-containing plants, dry leaves of Ipomoea marcellia containing 0.02% of swainsonine were administered to goats. Group I, with six goats, ingested 4g/kg of dry plant (0.8mg of swainsonina/kg) daily until the observation of the first neurologic signs. Two goats that did not receive the plant were used as control (Group II). Hepatic biopsies with the Menghini needle were performed by the percutaneous technique at day zero and at weekly intervals after the start of the administration of I, marcellia. Biopsy samples were fixed in 10% formaline, processed routinely, and stained by hematoxilin-eosin and by lectins histochemistry. Hepatocellular vacuolization similar to those described in cases of lysosomal storage disease were identified in all goats of Group I from the seven day of plant consumption in the samples satained with hematoxylin-eosin. Using lectin histochemistry, consistent labellings were observed with Concanavalla ensifonnis (Con-A) e Triticum vutgaris (WGA). It is concluded that routinely histological evaluation of liver biopsies can be used in the diagnosis of poisoning by swainsonine containing plants, even in goats without clinical signs, and lectin histochemistry which can be used as supplementary diagnostic method. C1 [Rocha, Brena P.] Univ Fed Rural Pernambuco UFRPE, Programa Posgrad Ciencia Vet, Rua Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil. [Reis, Matheus O.; Driemeier, David] Univ Fed Rio Grande do Sul, SPV, Dept Patol Clin Vet, BR-91540000 Porto Alegre, RS, Brazil. [Cook, Daniel] ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. [Camargo, Lazaro M.] Univ Cuiaba Unic, Fac Med Vet, Ave Manuel Jose de Arruda 3100, BR-78065900 Cuiaba, MT, Brazil. [Riet-Correa, Franklin] INIA, Estn Expt La Estanzuela, Ruta 50 Km 11, Colonia, Uruguay. [Evencio-Neto, Joaquim; Mendonca, Fabio S.] Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Lab Diagnest Anim, BR-52171900 Recife, PE, Brazil. RP Mendonca, FS (reprint author), Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Lab Diagnest Anim, BR-52171900 Recife, PE, Brazil. EM fabio.mendonca@pq.cnpq.br NR 18 TC 0 Z9 0 U1 2 U2 2 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X EI 1678-5150 J9 PESQUI VET BRASIL JI Pesqui. Vet. Bras. PD MAY PY 2016 VL 36 IS 5 BP 373 EP 377 DI 10.1590/S0100-736X2016000500003 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DS5PK UT WOS:000380834300003 ER PT J AU Graybosch, RA Hansen, LE AF Graybosch, Robert A. Hansen, Lavern E. TI Functionality of chemically modified waxy, partial waxy and wild-type starches from common wheat SO STARCH-STARKE LA English DT Article DE Amylose; Wheat; Functional properties; Modified starches ID WX PROTEIN; AMYLOSE; IDENTIFICATION; REGISTRATION; CULTIVARS AB Waxy wheat (Triticum aestivum L.) starch contains little or no amylose. Partial waxy wheats have amylose concentrations intermediate between those of waxy and wild-type (normal) starches. A factorial design was used to compare waxy, wild-type, partial waxy, and blends (10 and 17% amylose) of waxy and wild-type starches in native states, and after cross-linking with phosphorus oxychloride, substitution with propylene oxide, and a sequential modification procedure using both approaches. In analyses via the Rapid Visco Analyser (RVA), native waxy wheat starch reached peak viscosity at a significantly lower temperature, and displayed significantly higher viscosity (> 3000 cP), than starches of all other genotypes and blends. Hydroxypropylation of waxy wheat starch increased peak viscosity to > 3500 cP. In contrast, native and hydroxypropylated wild-type starches demonstrated peak viscosities of 1000 and 1500 cP, respectively. Cross-linked waxy starches demonstrated stable viscosities, with no breakdown or setback. With both native and modified starches, partial waxy pasting curves displayed viscosities intermediate between those of waxy and wild-type, though the same effect was obtained via use of the 17% blend. The 10% native starch blend displayed pasting properties intermediate between those of the partial waxy/17% blend group, and the waxy sample. The results suggest that appropriate selection of amylose contents, ideally formed by blending of waxy and wild-type starches, and chemical modification method, can result in wheat starches with predictable functional properties for a variety of food and industrial uses. C1 [Graybosch, Robert A.; Hansen, Lavern E.] ARS, USDA, Lincoln, NE USA. RP Graybosch, RA (reprint author), Univ Nebraska, USDA ARS, 137 Keim Hall,East Campus, Lincoln, NE 68583 USA. EM bob.graybosch@ars.usda.gov NR 23 TC 0 Z9 0 U1 5 U2 6 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 0038-9056 EI 1521-379X J9 STARCH-STARKE JI Starch-Starke PD MAY PY 2016 VL 68 IS 5-6 BP 496 EP 504 DI 10.1002/star.201500241 PG 9 WC Food Science & Technology SC Food Science & Technology GA DR8OF UT WOS:000380156500014 ER PT J AU He, HQ Arsenault, RJ Genovese, KJ Kogut, MH AF He, Haiqi Arsenault, Ryan J. Genovese, Kenneth J. Kogut, Michael H. TI Kinome analysis of chicken macrophages infected with Salmonella Enteritidis or S. Heidelberg SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [He, Haiqi; Genovese, Kenneth J.; Kogut, Michael H.] ARS, USDA, Washington, DC USA. [Arsenault, Ryan J.] Univ Delaware, Newark, DE 19716 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 MA 66.14 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288301015 ER PT J AU Kim, W Lillehoj, HS Fernandez, CP Min, W AF Kim, Woohyun Lillehoj, Hyun S. Fernandez, Cherry P. Min, Wongi TI Comparative analysis of expression profiles of cytokines in Riemerella anatipestifer infection in ducks and chickens highlights the role of IL-17A in bacterial susceptibility SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Kim, Woohyun; Lillehoj, Hyun S.] USDA ARS, Washington, DC 20250 USA. [Fernandez, Cherry P.; Min, Wongi] Gyeongsang Natl Univ, Jinju, South Korea. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303323 ER PT J AU Kim, W Lillehoj, HS Jeong, M Min, W Sullivan, YB Kakach, L LaBresh, JW AF Kim, Woohyun Lillehoj, Hyun S. Jeong, Misun Min, Wongi Sullivan, Yvonne B. Kakach, Laura LaBresh, Joanna W. TI Development and characterization of monoclonal antibodies specific for chicken IL-8 SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Kim, Woohyun; Lillehoj, Hyun S.; Jeong, Misun] USDA ARS, Washington, DC 20250 USA. [Min, Wongi] Gyeongsang Natl Univ, Jinju, South Korea. [Sullivan, Yvonne B.; Kakach, Laura; LaBresh, Joanna W.] Kingfisher Biotech Inc, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303312 ER PT J AU Levings, R AF Levings, Randall TI Antibody passive immunization against veterinary infectious disease SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Levings, Randall] Vet Serv, STAS, APHIS, USDA, El Segundo, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303313 ER PT J AU Maggioli, MF Palmer, MV Thacker, TT Vordermeier, M McGill, JL Larsen, MH Jacobs, WR Waters, WR AF Maggioli, Mayara Fernanda Palmer, Mitchell V. Thacker, Tyler T. Vordermeier, Martin McGill, Jodi L. Larsen, Michelle H. Jacobs, William R. Waters, W. Ray TI Polyfunctional cytokine production by central memory T cells from cattle in response to Mycobacterium bovis infection and BCG vaccination SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Maggioli, Mayara Fernanda] Iowa State Univ, Ames, IA USA. [Maggioli, Mayara Fernanda; Palmer, Mitchell V.; Thacker, Tyler T.; Waters, W. Ray] USDA ARS, Natl Anim Dis Ctr, Washington, DC 20250 USA. [Vordermeier, Martin] Anim & Plant Heath Agcy, London, England. [McGill, Jodi L.] Kansas State Univ, Manhattan, KS 66506 USA. [Larsen, Michelle H.; Jacobs, William R.] Albert Einstein Coll Med, New York, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303319 ER PT J AU Makkar, SK Liyanage, R Lay, JO Rath, NC AF Makkar, Sarbjeet K. Liyanage, Rohana Lay, Jackson O. Rath, Narayan C. TI Comparative proteomic analysis of chicken macrophages stimulated with Salmonella Lipopolysaccharide (LPS) and Monosodium Urate (MSU) Crystals SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Makkar, Sarbjeet K.; Liyanage, Rohana; Lay, Jackson O.] Univ Arkansas, Fayetteville, AR 72701 USA. [Makkar, Sarbjeet K.] USDA ARS, PPRSU, Washington, DC 20250 USA. [Rath, Narayan C.] Univ Arkansas, USDA ARS, PPRSU, Fayetteville, AR 72701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 MA 126.32 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288301310 ER PT J AU Miller, LC Sang, YM Bayles, DO Blecha, F AF Miller, Laura C. Sang, Yongming Bayles, Darrell O. Blecha, Frank TI Comparative analysis of signature genes in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)-infected porcine monocyte-derived dendritic cells at differential activation statuses SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Miller, Laura C.; Bayles, Darrell O.] USDA ARS, Natl Anim Dis Ctr, Washington, DC 20250 USA. [Sang, Yongming; Blecha, Frank] Kansas State Univ, Manhattan, KS 66506 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303328 ER PT J AU Rahe, MC Borgerding, AJ Wiseman, B Cenatti, L Martin, D Murtaugh, MP AF Rahe, Michael C. Borgerding, Anthony J. Wiseman, Barry Cenatti, Leonardo Martin, Daniel Murtaugh, Michael P. TI Establishment and characterization of a porcine lymphoma cell line SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Rahe, Michael C.; Borgerding, Anthony J.; Cenatti, Leonardo; Murtaugh, Michael P.] Univ Minnesota, Minneapolis, MN 55455 USA. [Wiseman, Barry] P VCS, Minneapolis, MN USA. [Martin, Daniel] USDA FSIS, Washington, DC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303318 ER PT J AU Sun, XH Wang, HC Qian, ML Zhao, Y Zhuang, Y Urban, JF Fung, KM AF Sun, Xiao-Hong Wang, Hong-Cheng Qian, Miranda Liangyue Zhao, Ying Zhuang, Yuan Urban, Joseph F. Fung, Kar-Ming TI Down-regulation of E protein activity augments an ILC2 differentiation program in the thymus SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Sun, Xiao-Hong; Wang, Hong-Cheng; Qian, Miranda Liangyue; Zhao, Ying] Oklahoma Med Res Fdn, 825 NE 13th St, Oklahoma City, OK 73104 USA. [Zhuang, Yuan] Duke Univ, Sch Med, Durham, NC 27706 USA. [Urban, Joseph F.] ARS, USDA, Washington, DC 20250 USA. [Fung, Kar-Ming] Univ Oklahoma, Hlth Sci Ctr, Norman, OK 73019 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 1 WC Immunology SC Immunology GA DS0LX UT WOS:000380288300472 ER PT J AU Sylte, MJ Torchetti, MK Killian, ML Spackman, E Pantin-Jackwood, MJ Kapczynski, DR Suarez, DL Swayne, DE AF Sylte, Matthew Jon Torchetti, Mia K. Killian, Mary L. Spackman, Erica Pantin-Jackwood, Mary J. Kapczynski, Darrell R. Suarez, David L. Swayne, David E. TI Efficacy of H5 subunit vaccines to protect poultry against highly pathogenic North American H5N2 avian influenza virus SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Sylte, Matthew Jon] USDA ARS, Natl Anim Dis Ctr, Washington, DC 20250 USA. [Torchetti, Mia K.; Killian, Mary L.] USDA APHIS, Nat Vet Serv Labs, Ames, IA USA. [Spackman, Erica; Pantin-Jackwood, Mary J.; Kapczynski, Darrell R.; Suarez, David L.; Swayne, David E.] USDA ARS, Southeast Poultry Res Lab, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303314 ER PT J AU Sylte, MJ Looft, TP Chandra, LC Bearson, SM Bearson, BL Susta, L AF Sylte, Matthew Jon Looft, Torey P. Chandra, Lawrance C. Bearson, Shawn M. Bearson, Bradely L. Susta, Leonardo TI Efficacy of an oral recombinant attennated Salmonella vaccine encoding cjaA in turkeys colonized with Campylobacter jejuni SO JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Association-of-Immunologists (AAI) CY MAY 13-17, 2016 CL Seattle, WA SP Amer Assoc Immunologists C1 [Sylte, Matthew Jon; Looft, Torey P.; Chandra, Lawrance C.; Bearson, Shawn M.] USDA ARS, Natl Anim Dis Ctr, Washington, DC 20250 USA. [Bearson, Bradely L.] USDA ARS, Washington, DC 20250 USA. [Susta, Leonardo] Univ Guelph, Guelph, ON N1G 2W1, Canada. NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 EI 1550-6606 J9 J IMMUNOL JI J. Immunol. PD MAY 1 PY 2016 VL 196 SU 1 PG 2 WC Immunology SC Immunology GA DS0LX UT WOS:000380288303317 ER PT J AU Zielinski, WJ Mazurek, MJ AF Zielinski, William J. Mazurek, M. J. TI Reproductive Characteristics of the Point Arena Mountain Beaver (Aplodontia rufa nigra) SO NORTHWEST SCIENCE LA English DT Article DE mountain beaver; Aplodontia; Point Arena; reproduction; breeding; California ID RANGE AB Little is known about the ecology and life history of the federally endangered Point Arena mountain beaver (Aplodontia rufa nigra). The distribution of this primitive burrowing rodent is disjunct from the balance of the species' range and occurs in a unique maritime environment of coastal grasslands and forests. Fundamental to protecting this taxon is knowledge of its breeding season and other reproductive characteristics. This information is necessary so that ground-disturbing activities near burrow systems can be scheduled to avoid the breeding season. We examined the reproductive status of 38 individuals, captured 150 times, from June 2004-July 2005 (excluding 01 February-01 May when we were prohibited from trapping). We used changes in vaginal cytology to monitor estrus and we evaluated male breeding readiness by determining whether testes were scrotal. The earliest onset of estrus occurred on 02 December and it was detected until the trapping prohibition period began on 01 February. The first males had scrotal testes somewhat earlier, in late November, and most males were still in this condition when trapping prohibition began. These data suggest an earlier onset of the breeding season than described for other subspecies. All females and a majority of males (70%) examined in December and January had evidence of reproductive condition. No evidence of pregnancy was observed during the study period, but based on allometric equations developed elsewhere, and applied to the weights of juveniles we captured, parturition was estimated to occur in early-to mid-April. C1 [Zielinski, William J.; Mazurek, M. J.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. RP Zielinski, WJ (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU United States Fish and Wildlife Service; Arcata field office; USDA Forest Service; Pacific Southwest Research Station; Redwood Sciences Laboratory; California Department of Fish and Wildlife FX This project was funded by the United States Fish and Wildlife Service, Arcata field office, the USDA Forest Service, Pacific Southwest Research Station, Redwood Sciences Laboratory and the California Department of Fish and Wildlife (Section 6 funds). We thank our field research assistant L. Hagenauer for her outstanding contributions to the project. We also thank field volunteers J. Bailey and R. Cozens. We thank R. Hamlin and J. Hunter of the USFWS Arcata Field Office for funds to initiate the project and also for their ideas and suggestions. Kim Fitts generously shared her expertise. We thank California State Parks for granting permission to conduct this project at Manchester State Park and are grateful to California State Parks Ecologist, R. Pasquinelli, and biologists, D. Roja and P. Warner for their cooperation. We are also grateful to State Parks staff D. Scully, K. Joe, D. Pitocco, and camp hosts C. and D. Churchill, and B. and J. Colburn. D. Nolte and W. Arjo, formerly with the USDA National Wildlife Research Center, generously shared their knowledge and equipment. Thanks also to D. Steele, California Department of Fish and Wildlife (retired), for his support and input. L. Diller, R. Feamster, K. Hamm (Green Diamond Resource Company) helped facilitate our pilot project. C. Ogan and R. Schlexer provided logistical support. J. Werren provided assistance creating maps and B. Howard helped with data management. NR 20 TC 0 Z9 0 U1 8 U2 8 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD MAY PY 2016 VL 90 IS 2 BP 136 EP 145 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DQ9JA UT WOS:000379525000002 ER PT J AU Mulvey, RL Bisbing, SM AF Mulvey, Robin L. Bisbing, Sarah M. TI Complex Interactions Among Agents Affect Shore Pine Health in Southeast Alaska SO NORTHWEST SCIENCE LA English DT Article DE shore pine; Pinus contorta; western gall rust; forest health monitoring; crown dieback ID WESTERN GALL RUST; LODGEPOLE PINE; CLIMATE-CHANGE; FOREST DECLINE; LIFE-HISTORY; SUSCEPTIBILITY; INFECTION; SELECTION; CONTORTA; MODELS AB Permanent plots are essential for tracking long-term forest change and have become more important given the projected increase in widespread tree mortality and forest health issues associated with climate change, invasive pests, altered disturbance regimes, and other novel stressors. Inventory and monitoring plots can reveal otherwise undetected loss of tree biomass, initiating targeted biological investigations. Shore pine is an understudied subspecies of lodgepole pine that reaches its northern extent in southeast Alaska. U.S. Forest Service Forest Inventory Analysis data detected a significant loss of live shore pine biomass in Alaska, with greater losses among larger trees and no known cause. We installed 46 permanent plots to monitor shore pine health and survival. Mortality was higher in shore pine (13%) than most associated conifers, and 43% of pines > 40 cm diameter at breast height were dead. Western gall rust, bole wounds, and Dothistroma needle blight were the most common forms of damage to live shore pine. Western gall rust bole gall presence best predicted crown dieback. Shore pine had more bole wounds than associated trees, with wound incidence and severity of live trees increasing with tree diameter. Secondary bark beetles and stain fungi were detected on dying and dead shore pine. Prevalent biotic injury and stressful site conditions accumulate, making large, old shore pine vulnerable to secondary bark beetle attack and vectored stain fungi. This study outlines how inventory networks can detect changes in tree biomass, highlighting knowledge gaps and prompting intensive, long-term monitoring. C1 [Mulvey, Robin L.] US Forest Serv, Forest Hlth Protect, USDA, Juneau Forestry Sci Lab, 11175 Auke Lake Way, Juneau, AK 99801 USA. [Bisbing, Sarah M.] Calif Polytech State Univ San Luis Obispo, Forest Ecol, 1 Grand Ave, San Luis Obispo, CA 93407 USA. RP Mulvey, RL (reprint author), US Forest Serv, Forest Hlth Protect, USDA, Juneau Forestry Sci Lab, 11175 Auke Lake Way, Juneau, AK 99801 USA. EM rlmulvey@fs.fed.us FU USDA Forest Service Forest Health Monitoring Program FX This project was funded by the USDA Forest Service Forest Health Monitoring Program. Tara Barrett conducted initial analyses of Forest Inventory and Analysis plot data that led to this project, and offered constructive input into plot layout and study design. Christy Cleaver, Sarah Navarro, and Melinda Lamb provided invaluable technical assistance with plot installation and other aspects of this project. Dr. Gavin Simpson provided statistical consultation and greatly improved statistical modeling methods. Elizabeth Graham and James Kruse identified insect specimens or facilitated identification, and conducted database searches to gather information on detected insects. Loretta Winton performed genetic sequencing of stain fungi and conks. The document was improved by a critical review by Paul Hennon. NR 63 TC 0 Z9 0 U1 5 U2 5 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD MAY PY 2016 VL 90 IS 2 BP 176 EP 194 PG 19 WC Ecology SC Environmental Sciences & Ecology GA DQ9JA UT WOS:000379525000005 ER PT J AU Koch, JB Looney, C Sheppard, WS Strange, JP AF Koch, Jonathan B. Looney, Chris Sheppard, Walter S. Strange, James P. TI Range Extension of Two Bumble Bee Species (Hymenoptera: Apidae) into Olympic National Park SO NORTHWEST SCIENCE LA English DT Article DE pollinator; MaxEnt; species distribution models; GBIF ID BOMBUS; CONSERVATION; DECLINE; CLIMATE; PATTERNS AB Bumble bees (Hymenoptera: Apidae, Bombus) are cold-adapted insects, primarily known for their importance in providing ecosystem services to wild and cultivated flowering plants. Recent expeditions into the wilderness regions of the Olympic Mountains of Olympic National Park, USA discovered undocumented populations of two bumble bee species: Bombus sylvicola and B. vandykei. Application of species distribution models with range-wide locality records identified the Olympic Mountains to have high habitat suitability for B. sylvicola and low habitat suitability for B. vandykei. Our results suggest that Olympic National Park is a habitat island for B. sylvicola, isolated from the relatively contiguous distribution of the species in the Cascade and Sierra Nevada mountain ranges. Bumble bees are sensitive to environmental change, thus our discoveries will likely stimulate conservation-oriented investigations on these charismatic pollinators on the Olympic Peninsula and throughout the Pacific Northwest. C1 [Koch, Jonathan B.] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. [Koch, Jonathan B.] Utah State Univ, Ctr Ecol, 5305 Old Main Hill, Logan, UT 84322 USA. [Looney, Chris] Washington State Dept Agr, Div Plant Protect, 1111 Washington St SE, Olympia, WA 98504 USA. [Sheppard, Walter S.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Strange, James P.] Utah State Univ, USDA ARS, Pollinating Insects Res Unit Biol Management Syst, BNR 255, Logan, UT 84322 USA. RP Koch, JB (reprint author), Univ Hawaii, Dept Biol, 200 W Kawili St, Hilo, HI 96720 USA.; Koch, JB (reprint author), Univ Hawaii, Trop Conservat Biol & Environm Sci Grad Program, 200 W Kawili St, Hilo, HI 96720 USA. EM kochj@hawaii.edu FU North Cascades and Coast Science Learning Network (NCCSLN) [NPS P13PG00149/FSN] FX We thank Dr. Brandon Hopkins, Craig Huntzinger, Houston Judd, Dr. Elinor Lichtenberg, and Jason Long for their assistance and patience during the intensive field surveys and Joyce Knoblett for laboratory support. We also thank Dr. Jerry Freilich and Dr. Regina Rochefort for their unwavering support in the study of bumble bees in National Parks. We thank Dr. David Inouye and one anonymous reviewer for their invaluable comments and suggestions that greatly improved this manuscript. This study was made possible with grant #NPS P13PG00149/FSN from the North Cascades and Coast Science Learning Network (NCCSLN) to Dr. James P. Strange and Dr. Walter S. Sheppard. NR 32 TC 0 Z9 0 U1 14 U2 17 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD MAY PY 2016 VL 90 IS 2 BP 228 EP 234 PG 7 WC Ecology SC Environmental Sciences & Ecology GA DQ9JA UT WOS:000379525000008 ER PT J AU Longland, WS Dimitri, LA AF Longland, William S. Dimitri, Lindsay A. TI Are Western Juniper Seeds Dispersed Through Diplochory? SO NORTHWEST SCIENCE LA English DT Article DE Endozoochory; Juniperus occidentalis; scatterhoarding; seed dispersal; seedling emergence ID RED CEDAR JUNIPERUS; VIRGINIANA; COMMUNIS; PASTURES; MOUNTAIN; INVASION; BERRIES; BIRDS AB Western juniper (Juniperus occidentalis) "berries" are consumed by frugivorous birds, which then defecate and disperse the seeds. However, rodents also consume western juniper seeds, and seedlings can establish from rodent scatterhoards. To explore relative roles of potential seed dispersal agents, we experimentally quantified removal rates by birds and rodents of intact western juniper berries versus seeds cleaned either manually or by passage through bird guts at two northeastern California sites (Likely and Shinn Peak). We also conducted seedling emergence experiments with berries, hand-cleaned, and bird-passed seeds placed on the soil surface or buried at a depth typical of rodent caches. Birds removed juniper berries from plots and left seeds undisturbed at Likely, but bird activity was negligible at Shinn Peak. Rodent removal of seeds versus berries was similar at Likely, but at Shinn Peak rodents removed significantly more seeds. In the seedling emergence experiment, juniper seeds or berries on the surface failed to produce seedlings. When buried, however, emergence was significantly greater for bird-passed than for hand-cleaned seeds, which both produced significantly more seedlings than intact berries. Our results indicate that: (1) birds may enhance western juniper seed germinability through gut passage, (2) most rodent species do not harvest intact berries, (3) rodents harvest seeds defecated by birds and may therefore secondarily disperse bird-passed seeds, and (4) seeds must be removed from berries and buried to establish seedlings. Sequential dispersal, or diplochory, with primary dispersal by frugivorous birds and secondary dispersal by seed-caching rodents, is ideal for satisfying these respective requirements. C1 [Longland, William S.] ARS, USDA, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. [Dimitri, Lindsay A.] Univ Nevada, Dept Biol, Reno, NV 89557 USA. RP Longland, WS (reprint author), ARS, USDA, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM bill.longland@ars.usda.gov NR 26 TC 0 Z9 0 U1 7 U2 8 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD MAY PY 2016 VL 90 IS 2 BP 235 EP 244 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DQ9JA UT WOS:000379525000009 ER PT J AU Nida, H Blum, S Zielinski, D Srivastava, DA Elbaum, R Xin, ZG Erlich, Y Fridman, E Shental, N AF Nida, Habte Blum, Shula Zielinski, Dina Srivastava, Dhruv A. Elbaum, Rivka Xin, Zhanguo Erlich, Yaniv Fridman, Eyal Shental, Noam TI Highly efficient de novo mutant identification in a Sorghum bicolor TILLING population using the ComSeq approach SO PLANT JOURNAL LA English DT Article DE Sorghum bicolor; Targeting induced local lesions in genomes; rare alleles; de novo SNPs; compressed sensing; large scale screening; technical advance ID INDUCED MUTATIONS; EXOME CAPTURE; DNA POOLS; DISCOVERY; SILICON; RICE; POLYMORPHISMS; TRANSPORTER; NUCLEOTIDE AB Screening large populations for carriers of known or de novo rare single nucleotide polymorphisms (SNPs) is required both in Targeting induced local lesions in genomes (TILLING) experiments in plants and in screening of human populations. We previously suggested an approach that combines the mathematical field of compressed sensing with next-generation sequencing to allow such large-scale screening. Based on pooled measurements, this method identifies multiple carriers of heterozygous or homozygous rare alleles while using only a small fraction of resources. Its rigorous mathematical foundations allow scalable and robust detection, and provide error correction and resilience to experimental noise. Here we present a large-scale experimental demonstration of our computational approach, in which we targeted a TILLING population of 1024 Sorghum bicolor lines to detect carriers of de novo SNPs whose frequency was less than 0.1%, using only 48 pools. Subsequent validation confirmed that all detected lines were indeed carriers of the predicted mutations. This novel approach provides a highly cost-effective and robust tool for biologists and breeders to allow identification of novel alleles and subsequent functional analysis. Significance Statement To improve plant productivity, it is desirable to develop more efficient methods to enrich the allelic repertoire used for breeding. Here, using compressed sequencing (ComSeq) with a Sorghum bicolor TILLING population, we detected and validated carriers of rare de novo SNPs. C1 [Nida, Habte; Blum, Shula; Srivastava, Dhruv A.; Elbaum, Rivka] Hebrew Univ Jerusalem, Robert H Smith Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, Rehovot, Israel. [Zielinski, Dina; Erlich, Yaniv] New York Genome Ctr, 101 Ave Amer, New York, NY USA. [Srivastava, Dhruv A.; Fridman, Eyal] Agr Res Org, Volcani Ctr, Inst Plant Sci, Bet Dagan, Israel. [Xin, Zhanguo] ARS, Plant Stress & Germplasm Dev Unit, USDA, Lubbock, TX USA. [Erlich, Yaniv] Columbia Univ, Dept Comp Sci, Fu Fdn, Sch Engn, New York, NY 10027 USA. [Erlich, Yaniv] Columbia Univ, Ctr Computat Biol & Bioinformat, New York, NY USA. [Shental, Noam] Open Univ Israel, Dept Math & Comp Sci, Raanana, Israel. RP Fridman, E (reprint author), Agr Res Org, Volcani Ctr, Inst Plant Sci, Bet Dagan, Israel.; Shental, N (reprint author), Open Univ Israel, Dept Math & Comp Sci, Raanana, Israel. EM fridmane@agri.gov.il; shental@openu.ac.il FU US/Israel Binational Agricultural Research and Development Fund [IS-4447-11F]; Israeli Ministry of Agriculture and Rural Development [837-0127-11]; Pears Foundation (UK) FX This research was supported by research grant award number IS-4447-11F from the US/Israel Binational Agricultural Research and Development Fund, and by research grant award number 837-0127-11 from the Israeli Ministry of Agriculture and Rural Development. H.N. was supported by the Pears Foundation (UK). We would like to thank the Whitehead Institute Genome Technology Core for their assistance in sequencing. NR 29 TC 1 Z9 1 U1 9 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD MAY PY 2016 VL 86 IS 4 BP 349 EP 359 DI 10.1111/tpj.13161 PG 11 WC Plant Sciences SC Plant Sciences GA DR0LG UT WOS:000379598400005 PM 26959378 ER PT J AU Guo, M Mishra, A Buchanan, RL Dubey, JP Hill, DE Gamble, HR Jones, JL Du, XZ Pradhan, AK AF Guo, Miao Mishra, Abhinav Buchanan, Robert L. Dubey, Jitender P. Hill, Dolores E. Gamble, H. Ray Jones, Jeffrey L. Du, Xianzhi Pradhan, Abani K. TI Development of Dose-Response Models to Predict the Relationship for Human Toxoplasma gondii Infection Associated with Meat Consumption SO RISK ANALYSIS LA English DT Article DE Dose-response; human infection; meat; quantitative microbial risk assessment; Toxoplasma gondii ID INDUCED MURINE TOXOPLASMOSIS; UNITED-STATES; RISK-ASSESSMENT; CONGENITAL TOXOPLASMOSIS; OCULAR TOXOPLASMOSIS; EXPERT ELICITATION; STAGE CONVERSION; OOCYSTS; MICE; BRADYZOITES AB Toxoplasma gondii is a protozoan parasite that is responsible for approximately 24% of deaths attributed to foodborne pathogens in the United States. It is thought that a substantial portion of human T. gondii infections is acquired through the consumption of meats. The dose-response relationship for human exposures to T. gondii-infected meat is unknown because no human data are available. The goal of this study was to develop and validate dose-response models based on animal studies, and to compute scaling factors so that animal-derived models can predict T. gondii infection in humans. Relevant studies in literature were collected and appropriate studies were selected based on animal species, stage, genotype of T. gondii, and route of infection. Data were pooled and fitted to four sigmoidal-shaped mathematical models, and model parameters were estimated using maximum likelihood estimation. Data from a mouse study were selected to develop the dose-response relationship. Exponential and beta-Poisson models, which predicted similar responses, were selected as reasonable dose-response models based on their simplicity, biological plausibility, and goodness fit. A confidence interval of the parameter was determined by constructing 10,000 bootstrap samples. Scaling factors were computed by matching the predicted infection cases with the epidemiological data. Mouse-derived models were validated against data for the dose-infection relationship in rats. A human dose-response model was developed as P (d) = 1-exp (-0.0015 x 0.005 x d) or P (d) = 1-(1 + d x 0.003 / 582.414)(-1.479). Both models predict the human response after consuming T. gondii-infected meats, and provide an enhanced risk characterization in a quantitative microbial risk assessment model for this pathogen. C1 [Guo, Miao; Mishra, Abhinav; Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Buchanan, Robert L.; Pradhan, Abani K.] Univ Maryland, Ctr Food Safety & Secur Syst, College Pk, MD 20742 USA. [Dubey, Jitender P.; Hill, Dolores E.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD USA. [Gamble, H. Ray] Natl Acad Sci, 2101 Constitut Ave Nw, Washington, DC 20418 USA. [Jones, Jeffrey L.] Ctr Dis Control & Prevent, Atlanta, GA USA. [Du, Xianzhi] Univ Maryland, Dept Elect & Comp Engn, College Pk, MD 20742 USA. RP Pradhan, AK (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM akp@umd.edu OI Mishra, Abhinav/0000-0001-9214-0745 FU USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative [2012-67005-19611] FX This work was supported through a grant from the USDA National Institute of Food and Agriculture (NIFA) Agriculture and Food Research Initiative (award 2012-67005-19611). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the National Institute of Food and Agriculture, the Centers for Disease Control and Prevention, or the U.S. Department of Agriculture. NR 51 TC 1 Z9 1 U1 2 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD MAY PY 2016 VL 36 IS 5 BP 926 EP 938 DI 10.1111/risa.12500 PG 13 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA DR2MF UT WOS:000379737900009 PM 26477997 ER PT J AU Zagmutt, FJ Schoenbaum, MA Hill, AE AF Zagmutt, Francisco J. Schoenbaum, Mark A. Hill, Ashley E. TI The Impact of Population, Contact, and Spatial Heterogeneity on Epidemic Model Predictions SO RISK ANALYSIS LA English DT Article DE Disease modeling; health policy; parsimony ID MOUTH-DISEASE; PANDEMIC INFLUENZA; SIMULATION-MODEL; UNITED-STATES; FOOT; TRANSMISSION; OUTBREAK; SPREAD; STRATEGIES; RATES AB Our objective was to evaluate the effect that complexity in the form of different levels of spatial, population, and contact heterogeneity has in the predictions of a mechanistic epidemic model. A model that simulates the spatiotemporal spread of infectious diseases between animal populations was developed. Sixteen scenarios of foot-and-mouth disease infection in cattle were analyzed, involving combinations of the following factors: multiple production-types (PT) with heterogeneous contact and population structure versus single PT, random versus actual spatial distribution of population units, high versus low infectivity, and no vaccination versus preemptive vaccination. The epidemic size and duration was larger for scenarios with multiple PT versus single PT. Ignoring the actual unit locations did not affect the epidemic size in scenarios with multiple PT/high infectivity, but resulted in smaller epidemic sizes in scenarios using multiple PT/low infectivity. In conclusion, when modeling fast-spreading epidemics, knowing the actual locations of population units may not be as relevant as collecting information on population and contact heterogeneity. In contrast, both population and spatial heterogeneity might be important to model slower spreading epidemic diseases. Our findings can be used to inform data collection and modeling efforts to inform health policy and planning. C1 [Zagmutt, Francisco J.] EpiX Analyt, 1643 Spruce St, Boulder, CO 80302 USA. [Schoenbaum, Mark A.] USDA APHIS, Ft Collins, CO USA. [Hill, Ashley E.] Univ Calif Davis, Davis, CA USA. RP Zagmutt, FJ (reprint author), EpiX Analyt, 1643 Spruce St, Boulder, CO 80302 USA. EM fzagmutt@epixanalytics.com NR 31 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD MAY PY 2016 VL 36 IS 5 BP 939 EP 953 DI 10.1111/risa.12482 PG 15 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA DR2MF UT WOS:000379737900010 PM 26477887 ER PT J AU Chantawannakul, P de Guzman, LI Li, JL Williams, GR AF Chantawannakul, Panuwan de Guzman, Lilia I. Li, Jilian Williams, Geoffrey R. TI Parasites, pathogens, and pests of honeybees in Asia SO APIDOLOGIE LA English DT Review DE Asia; bee virus; Tropilaelaps; Asian beemites; honeybees; bee diseases ID APIS-MELLIFERA COLONIES; VARROA-JACOBSONI OUDEMANS; MITE TROPILAELAPS-CLAREAE; MULTIVARIATE MORPHOMETRIC-ANALYSIS; EUVARROA-SINHAI DELFINADO; QUEEN-CELL-VIRUS; BEE-PARALYSIS-VIRUS; DEFORMED WING VIRUS; PAPUA-NEW-GUINEA; NOSEMA-CERANAE AB Asia is home to at least nine honeybee species, including the introduced Apis mellifera. In addition to A. mellifera and Apis cerana being widely employed for commercial beekeeping, the remaining nonmanaged species also have important ecological and economic roles on the continent. Species distributions of most honeybee species overlap in Southeast Asia. This promotes the potential for interspecific transmission of pests and parasites and their spread to other parts of the world by human translocation. The decline of honeybee populations is of great concern around the world, including in Asia. The global colony losses of A. mellifera are believed to be caused, in part, by parasites, pathogens, and pests originating from Asia, such as the mite Varroa destructor, themicrosporidian Nosema ceranae, and some bee viruses. This review discusses important pests, pathogens, and parasites in both the introduced A. mellifera and native honeybees in Asia to provide an overall picture of honeybee health in the region and future threats to the apiculture industry. C1 [Chantawannakul, Panuwan] Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Lab BeeP, Chiang Mai 50200, Thailand. [de Guzman, Lilia I.] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. [Li, Jilian] Chinese Acad Agr Sci, Inst Apicultural Res, Minist Agr, Key Lab Pollinating Insect Biol, Beijing 100093, Peoples R China. [Williams, Geoffrey R.] Univ Bern, Inst Bee Hlth, Vetsuisse Fac, CH-3003 Bern, Switzerland. [Williams, Geoffrey R.] Swiss Bee Res Ctr, Agroscope, CH-3003 Bern, Switzerland. RP Chantawannakul, P (reprint author), Chiang Mai Univ, Fac Sci, Dept Biol, Bee Protect Lab BeeP, Chiang Mai 50200, Thailand. EM panuwan@gmail.com NR 227 TC 1 Z9 1 U1 17 U2 27 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAY PY 2016 VL 47 IS 3 BP 301 EP 324 DI 10.1007/s13592-015-0407-5 PG 24 WC Entomology SC Entomology GA DQ1ZO UT WOS:000379000100003 ER PT J AU Traynor, KS Rennich, K Forsgren, E Rose, R Pettis, J Kunkel, G Madella, S Evans, J Lopez, D vanEngelsdorp, D AF Traynor, Kirsten S. Rennich, Karen Forsgren, Eva Rose, Robyn Pettis, Jeffery Kunkel, Grace Madella, Shayne Evans, Jay Lopez, Dawn vanEngelsdorp, Dennis TI Multiyear survey targeting disease incidence in US honey bees SO APIDOLOGIE LA English DT Article DE Apis mellifera; disease survey; pests; parasite; USA ID DEFORMED WING VIRUS; MITE VARROA-JACOBSONI; APIS-MELLIFERA; COLONY LOSSES; NATIONAL-SURVEY; NOSEMA-CERANAE; DESTRUCTOR; REPLICATION; PREVALENCE; MORTALITY AB The US National Honey Bee Disease Survey sampled colony pests and diseases from 2009 to 2014. We verified the absence of Tropilaelaps spp., the Asian honey bee (Apis cerana), and slow bee paralysis virus. Endemic health threats were quantified, including Varroa destructor, Nosema spp., and eight honey bee viruses. Varroa loads varied across years, with annual fall peaks; Nosema peaked January to April. Migratory beekeepers had significantly lower Varroa prevalence (84.9 vs. 97.0 %) and loads (3.65 +/- 0.28 vs. 5.99 +/- 0.22) than stationary operations, while Nosema was more prevalent (59.9 vs. 46.7 %) in migratory colonies. Since 2010, chronic bee paralysis virus prevalence doubled annually. We detected strong positive relationships between V. destructor and Varroa -transmitted viruses, between Nosema and Lake Sinai virus 2, and a positive relationship across several viral pathogens of bees. The results provide a disease baseline to help identify drivers of poor bee health. C1 [Traynor, Kirsten S.; Rennich, Karen; Forsgren, Eva; Kunkel, Grace; Madella, Shayne; vanEngelsdorp, Dennis] Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. [Forsgren, Eva] Swedish Univ Agr Sci, Dept Ecol, POB 7044, S-75007 Uppsala, Sweden. [Rose, Robyn] US Anim & Plant Hlth Inspect Serv, USDA, 4700 River Rd, Riverdale, MD 20737 USA. [Pettis, Jeffery; Evans, Jay; Lopez, Dawn] USDA ARS, Beltsville Agr Res Ctr East, Bldg 306, Beltsville, MD 20705 USA. RP vanEngelsdorp, D (reprint author), Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. EM dvane@umd.edu OI Evans, Jay/0000-0002-0036-4651 FU USDA APHIS FX We would like to thank USDA APHIS for assistance with coordination and funding of the survey and Apiary Inspectors of America for collecting the samples in the field. We would also like to thank the following for support in sample collection, kit construction, sample processing, and analysis: Sam Abban, Michael Andree, Tyler Conine, Heather Eversole, Rachel Fahey, Andrew Garavito, Ashley Jones, Vic Levi, Meghan McConnell, Anthony Nearman, Nishit Patel, Nathan Rice, Karen Roccasecca, Bart Smith Jr., Margaret Smith, Steven Smith, Robert Snyder, Nathalie Steinhauer, and Jennie Stitzinger. NR 59 TC 4 Z9 4 U1 15 U2 21 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAY PY 2016 VL 47 IS 3 BP 325 EP 347 DI 10.1007/s13592-016-0431-0 PG 23 WC Entomology SC Entomology GA DQ1ZO UT WOS:000379000100004 ER PT J AU Neumann, P Pettis, JS Schazfer, MO AF Neumann, Peter Pettis, Jeff S. Schaezfer, Marc O. TI Quo vadis Aethina tumida ? Biology and control of small hive beetles SO APIDOLOGIE LA English DT Review DE Apis mellifera; Aethina tumida; bees; invasive species; small hive beetle ID HONEY-BEE COLONIES; APIS-MELLIFERA-CAPENSIS; MURRAY COLEOPTERA NITIDULIDAE; DESTRUCTOR ACARI VARROIDAE; BOTTOM BOARD TRAPS; SCIENTIFIC NOTE; HYMENOPTERA APIDAE; BOMBUS-IMPATIENS; KODAMAEA-OHMERI; UNITED-STATES AB Small hive beetles (SHBs) are generalists native to sub-Saharan Africa and reproduce in association with honeybees, bumblebees, stingless bees, fruits and meat. The SHB has recently become an invasive species, and introductions have been recorded from America, Australia, Europe and Asia since 1996. While SHBs are usually considered a minor pest in Africa, they can cause significant damage to social bee colonies in their new ranges. Potential reasons for differential impact include differences in bee behaviour, climate and release from natural enemies. Here, we provide an overview on biology, distribution, pest status, diagnosis, control and prevention to foster adequate mitigation and stimulate future research. SHBs have become a global threat to both apiculture and wild bee populations, but our knowledge of this pest is still limited, creating demand for more research in all areas of its biology. C1 [Neumann, Peter] Univ Bern, Vetsuisse Fac, Inst Bee Hlth, Bern, Switzerland. [Neumann, Peter] Swiss Bee Res Ctr, Agroscope, Bern, Switzerland. [Neumann, Peter] Univ Pretoria, Dept Zool & Entomol, Social Insect Res Grp, Pretoria, South Africa. [Pettis, Jeff S.] USDA ARS, Bee Res Lab, Beltsville, MD USA. [Schaezfer, Marc O.] Fed Res Inst Anim Hlth, FLI, Natl Reference Lab Bee Dis, Greifswald, Insel Riems, Germany. RP Neumann, P (reprint author), Univ Bern, Vetsuisse Fac, Inst Bee Hlth, Bern, Switzerland.; Neumann, P (reprint author), Swiss Bee Res Ctr, Agroscope, Bern, Switzerland.; Neumann, P (reprint author), Univ Pretoria, Dept Zool & Entomol, Social Insect Res Grp, Pretoria, South Africa. EM peter.neumann@vetsuisse.unibe.ch FU Vinetum Foundation FX We wish to thank Nicholas Annand, Alexander Kirejtshuk and an anonymous referee for constructive comments, Wolfgang Nentwig for stimulating discussions as well as Daniel Schlappi for editing an earlier version of the manuscript. Bram Cornelissen and Loris Gbedjissi kindly provided unpublished information. We are particularly grateful to Nicholas Annand, Cleofas R. Cervancia, Lilia de Guzman, Danielle Downey, Rafael A. Calderon Fallas, Pierre Giovenazzo, Yasser Ibrahim, Natalia Escobedo Kenefic, Alejandro Nicol, Antoine Milhau, Hug Smith and Robert Spooner-Hart for detailed updates from Australia, Canada, Egypt, Hawaii, Jamaica, the Philippines and Central America. Financial support was granted by the Vinetum Foundation to PN. NR 244 TC 4 Z9 4 U1 15 U2 25 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAY PY 2016 VL 47 IS 3 BP 427 EP 466 DI 10.1007/s13592-016-0426-x PG 40 WC Entomology SC Entomology GA DQ1ZO UT WOS:000379000100009 ER PT J AU Danka, RG Harris, JW Dodds, GE AF Danka, Robert G. Harris, Jeffery W. Dodds, Garrett E. TI Selection of VSH- derived "Pol-line" honey bees and evaluation of their Varroa -resistance characteristics SO APIDOLOGIE LA English DT Article DE Apis mellifera; Varroa destructor; mite resistance; Varroa sensitive hygiene; breeding ID DESTRUCTOR RESEARCH AVENUES; SENSITIVE HYGIENE VSH; JACOBSONI MESOSTIGMATA; SUSTAINABLE CONTROL; HYMENOPTERA APIDAE; RESPONSES; COLONIES; BROOD AB Honey bees with Varroa sensitive hygiene (VSH) have good resistance to Varroa destructor. We bred "Pol-line" bees by outcrossing VSH queens to US commercial stocks from 2008 to 2014 and then selecting colonies with low mite infestations. Beginning in 2011, field performance of colonies with outcrossed Pol-line queens was compared to colonies with outcrossed VSH queens. Mite infestations after one season were comparable in colonies of the two bee types. Queens from the most functional colonies of both bee types were added to the Pol-line breeding population each year. Mite resistance was investigated further by exposing mite-infested brood to colonies for 1 week in lab tests. The two bee types did not differ in the percentage of infested brood they removed or in the percentage of non-reproduction among remaining mites. Introgressing the VSH trait into commercial honey bee stock shows promise in creating bees that have useful mite resistance and desirable beekeeping characteristics. C1 [Danka, Robert G.; Dodds, Garrett E.] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. [Harris, Jeffery W.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Starkville, MS 39762 USA. RP Danka, RG (reprint author), USDA ARS, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM Bob.Danka@ars.usda.gov FU National Honey Board FX We thank David Dodge, Victor Rainey, and Daniel Winfrey (USDA-ARS) for excellent technical assistance, the three beekeeping companies for dedicated collaboration, Debbie Boykin (USDA-ARS) for statistical advice, Jose Villa (USDA-ARS), and BartJan Fernhout (Arista Bee Research Foundation) for helpful discussion, and the National Honey Board for funding. NR 21 TC 0 Z9 0 U1 9 U2 11 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD MAY PY 2016 VL 47 IS 3 BP 483 EP 490 DI 10.1007/s13592-015-0413-7 PG 8 WC Entomology SC Entomology GA DQ1ZO UT WOS:000379000100011 ER PT J AU Adekanmbi, EO Ueti, MW Rinaldi, B Suarez, CE Srivastava, SK AF Adekanmbi, Ezekiel O. Ueti, Massaro W. Rinaldi, Brady Suarez, Carlos E. Srivastava, Soumya K. TI Insulator-based dielectrophoretic diagnostic tool for babesiosis SO BIOMICROFLUIDICS LA English DT Article; Proceedings Paper CT Annual Meeting of the AES-Electrophoresis-Society CY 2015 CL Salt Lake, UT SP AES Electrophoresis Soc ID NEW-YORK; MICROTI; MALARIA; BOVIS; PARASITEMIA; ANTIBODIES; SEPARATION; INFECTION; INSIGHTS AB Babesia species are obligate intraerythrocytic tick-borne protozoan parasites that are the etiologic agents of babesiosis, a potentially life-threatening, malaria-like illness in humans and animals. Babesia-infected people have been known to suffer from complications including liver problems, severe hemolytic anemia, and kidney failure. As reported by the Food and Drug Administration, 38% of mortality cases observed in transfusion recipients were associated with transfusion transmitted diseases of which babesiosis is the chief culprit. As of now, no tests have been licensed yet for screening blood donors for babesiosis. Current diagnostic tools for babesiosis including enzyme-linked immunosorbent assay, fluorescence in situ hybridization, and polymerase chain reaction are expensive and burdened with multifarious shortcomings. In this research, a low-cost, high-specificity, quick, and easy-to-use insulator-based dielectrophoretic diagnostic tool is developed for characterizing and concentrating Babesia-infected cells in their homogenous mixture with healthy cell population. In this work, a mixture of Babesia-infected (varying parasitemia) and healthy red blood cells (RBCs or erythrocytes) was exposed to non-uniform electric fields in a fabricated microfluidic platform to manipulate and sort the Babesia-infected cells within a minute. At DC voltage configurations of 10V and 0/6V in the inlet and the two outlet channels, respectively, the diseased cells were seen to flow in a direction different from the healthy RBCs. Bright field and fluorescence microscopy were utilized to present qualitative differentiation of the healthy erythrocytes from the infected cells. The proposed micro device platform was able to enrich RBCs from 0.1% to similar to 70% parasitemia. This device, when finally developed into a point-of-care diagnostic chip, would enhance the detection of Babesia-infected erythrocytes and as well serve as a precursor to babesiosis vaccine development. Published by AIP Publishing. C1 [Adekanmbi, Ezekiel O.; Rinaldi, Brady; Srivastava, Soumya K.] Univ Idaho, Dept Chem & Mat Engn, Moscow, ID 83844 USA. [Ueti, Massaro W.; Suarez, Carlos E.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. RP Srivastava, SK (reprint author), Univ Idaho, Dept Chem & Mat Engn, Moscow, ID 83844 USA. EM srivastavask@uidaho.edu RI Adekanmbi, Ezekiel/F-9050-2015 OI Adekanmbi, Ezekiel/0000-0002-5103-9883 NR 36 TC 1 Z9 1 U1 2 U2 4 PU AMER INST PHYSICS PI MELVILLE PA 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA SN 1932-1058 J9 BIOMICROFLUIDICS JI Biomicrofluidics PD MAY PY 2016 VL 10 IS 3 AR 033108 DI 10.1063/1.4954196 PG 12 WC Biochemical Research Methods; Biophysics; Nanoscience & Nanotechnology; Physics, Fluids & Plasmas SC Biochemistry & Molecular Biology; Biophysics; Science & Technology - Other Topics; Physics GA DQ4GP UT WOS:000379162400013 PM 27375817 ER PT J AU Inman-Narahari, F Ostertag, R Hubbell, SP Giardina, CP Cordell, S Sack, L AF Inman-Narahari, Faith Ostertag, Rebecca Hubbell, Stephen P. Giardina, Christian P. Cordell, Susan Sack, Lawren TI Density-dependent seedling mortality varies with light availability and species abundance in wet and dry Hawaiian forests SO JOURNAL OF ECOLOGY LA English DT Article DE community compensatory trend; density dependence; determinants of plant community diversity and structure; facilitation; Janzen-Connell hypothesis; regeneration dynamics; seedling ecology; tropical dry forest; tropical wet forest ID TROPICAL TREE SEEDLINGS; RAIN-FOREST; LOCAL NEIGHBORHOOD; NEOTROPICAL FOREST; WOODY SEEDLINGS; SPATIAL-PATTERN; SHADE TOLERANCE; TRADE-OFFS; SURVIVAL; COEXISTENCE AB 1. Conspecific density may contribute to patterns of species assembly through negative density dependence (NDD) as predicted by the Janzen-Connell hypothesis, or through facilitation (positive density dependence; PDD). Conspecific density effects are expected to be more negative in darker and wetter environments due to higher pathogen abundance and more positive in stressful, especially dry, environments (stress-gradient hypothesis). For NDD to contribute to maintaining diversity, it should be apparent at the community-wide scale as a negative correlation between seedling recruitment, growth or survival and conspecific adult abundance (community compensatory trend; CCT). 2. We examined seedling survival in relation to con-and heterospecific adults within 10 m and con-and heterospecific seedlings within 1 m for 13 species within two 4-ha permanent plots located in dry and wet forests in Hawaii. We also examined interactions between conspecific density and light and species' commonness. 3. For all species pooled, adult conspecific effects were positive (PDD) in both dry and wet forests, though they were stronger in the dry forest. In contrast, seedling conspecific effects were negative (NDD), though only significantly so in the wet forest. 4. The strength and direction of density effects varied with understorey light such that seedlings had the highest survival where both adult conspecific density and light were high but the lowest survival where seedling conspecific density and light were high. 5. In the wet forest, the most common species showed positive effects of adult conspecifics, but the less common species showed negative adult conspecific effects. We found mixed evidence for a CCT: seedling survival was positively correlated with basal area, but negatively correlated with tree density (stems ha(-1)). Thus, it remains unclear whether NDD is a diversity-maintaining mechanism in these forests. 6. Synthesis. Overall, we found that positive conspecific effects influenced seedling mortality patterns more than negative interactions did, even in tropical wet forest where NDD is predicted to drive species' abundances. Additionally, the strength and direction of density effects varied with forest type, PAR, and species' abundance, underscoring the need to consider abiotic factors and species' life-history traits in tests of density dependence hypotheses. C1 [Inman-Narahari, Faith; Hubbell, Stephen P.; Sack, Lawren] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, 621 Charles E Young Dr South, Los Angeles, CA 90095 USA. [Ostertag, Rebecca] Univ Hawaii, Dept Biol, 200 W Kawili St, Hilo, HI 96720 USA. [Hubbell, Stephen P.] Smithsonian Trop Res Inst, Ctr Trop Forest Sci, Box 2072, Balboa, Panama. [Giardina, Christian P.; Cordell, Susan] US Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, 60 Nowelo St, Hilo, HI 96720 USA. RP Inman-Narahari, F (reprint author), Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, 621 Charles E Young Dr South, Los Angeles, CA 90095 USA. EM finman@hawaii.edu RI Giardina, Christian/C-3120-2011; Sack, Lawren/A-5492-2008 OI Giardina, Christian/0000-0002-3431-5073; Sack, Lawren/0000-0002-7009-7202 FU National Science Foundation [EPSCoR 0554657, 0903833, IOS-0546784]; Smithsonian Tropical Research Institute Center for Tropical Forest Science; University of Hawaii; USDA Forest Service Pacific Southwest Research Station Institute of Pacific Islands Forestry (USFS-IPIF); Catherine H. Beattie grant from the Garden Club of America; Vavra and Pauley Fellowships from the University of California, Los Angeles FX We thank the many field assistants who collected seedling data, especially Molly Murphy, Kahealani Wailani-Nihipali, Kehauwealani Nelson-Kaula and others with the Hawaii Community College Tropical Forest Ecosystem and Agroforestry Management and University of Hawaii at Hilo Pacific Internship Programs for Exploring Science programs. We thank Bernice Hwang, Michael Nullet, Paul Scowcroft and Jodie Schulten for assistance with logistics. We are grateful to Megan Bartlett, Marissa Caringella, Grace John, Rodrigo Mendez-Alonzo, Christine Scoffoni, Daniel J. Johnson, Margaret Metz, Nikhil Inman-Narahari, and anonymous reviewers for comments on draft versions of the manuscript. Financial support was provided by the National Science Foundation (Grants EPSCoR 0554657 and 0903833, and IOS-0546784), the Smithsonian Tropical Research Institute Center for Tropical Forest Science, the University of Hawaii, the USDA Forest Service Pacific Southwest Research Station Institute of Pacific Islands Forestry (USFS-IPIF), a Catherine H. Beattie grant from the Garden Club of America, and Vavra and Pauley Fellowships from the University of California, Los Angeles. We thank the USFS-IPIF and the Hawaii Division of Forestry and Wildlife/Department of Land and Natural Resources for access to the Hawaii Experimental Tropical Forest. NR 54 TC 0 Z9 0 U1 19 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD MAY PY 2016 VL 104 IS 3 BP 773 EP 780 DI 10.1111/1365-2745.12553 PG 8 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA DQ2EO UT WOS:000379014900016 ER PT J AU Nguyen, T Marini, J Stoll, B Didelija, I Orellana, R Agarwal, U Yuan, Y Desai, M Bashir, D Lam, F Vijayan, V Mason, E Lamberth, L Vallejo, J Kaplan, S Cruz, M AF Nguyen, T. Marini, J. Stoll, B. Didelija, I Orellana, R. Agarwal, U. Yuan, Y. Desai, M. Bashir, D. Lam, F. Vijayan, V. Mason, E. Lamberth, L. Vallejo, J. Kaplan, S. Cruz, M. TI Porcine model of methicillin-resistant staphylococcus aureus (MRSA) sepsis-induced disseminated intravascular coagulation (DIC) SO JOURNAL OF THROMBOSIS AND HAEMOSTASIS LA English DT Meeting Abstract C1 [Nguyen, T.; Marini, J.; Stoll, B.; Didelija, I; Orellana, R.; Agarwal, U.; Yuan, Y.; Desai, M.; Bashir, D.; Lam, F.; Mason, E.; Lamberth, L.; Vallejo, J.; Kaplan, S.] Texas Childrens Hosp, Baylor Coll Med, Pediat, Houston, TX 77030 USA. [Nguyen, T.; Bashir, D.; Lam, F.; Vijayan, V.; Cruz, M.] Michael E DeBakey VA Med Ctr, CTRID, Houston, TX USA. [Marini, J.; Stoll, B.; Didelija, I; Orellana, R.; Agarwal, U.; Yuan, Y.] ARS, USDA, Childrens Nutr Res Ctr, Houston, TX USA. [Vijayan, V.; Cruz, M.] Baylor Coll Med, Med, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1538-7933 EI 1538-7836 J9 J THROMB HAEMOST JI J. Thromb. Haemost. PD MAY PY 2016 VL 14 SU 1 SI SI MA DIC01 BP 35 EP 35 PG 1 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA DQ4HF UT WOS:000379164000084 ER PT J AU Miao, HR Fooks, JR Guilfoos, T Messer, KD Pradhanang, SM Suter, JF Trandafir, S Uchida, E AF Miao, Haoran Fooks, Jacob R. Guilfoos, Todd Messer, Kent D. Pradhanang, Soni M. Suter, Jordan F. Trandafir, Simona Uchida, Emi TI The impact of information on behavior under an ambient-based policy for regulating nonpoint source pollution SO WATER RESOURCES RESEARCH LA English DT Article DE nonpoint source pollution; ambient-based policy; information; laboratory economic experiment; river and stream water quality model ID POINT-SOURCE POLLUTION; INSTRUMENTS; PERFORMANCE; MECHANISMS; BLOOMS; TAXES; LAB AB Stemming from Segerson [1988], literature on nonpoint source pollution shows that ambient-based regulatory policies can induce polluters in a common watershed to comply with an exogenously determined pollution standard. This study uses laboratory economic experiments in a spatially heterogeneous setting to test the effectiveness of an ambient tax/subsidy policy in a setting with realistic in-stream nutrient transport dynamics when varying levels of sensor information on ambient pollution are available to the agents and the regulator. We find that increasing the frequency of ambient monitoring improves the spatial allocation of emissions reductions. In particular, with more frequent monitoring, the ambient-based policy induces firms further from the monitoring point to reduce emissions significantly more than downstream firms. Overall, the results suggest that enhanced temporal resolution of monitoring leads to efficiency gains. C1 [Miao, Haoran; Guilfoos, Todd; Trandafir, Simona; Uchida, Emi] Univ Rhode Isl, Dept Environm & Nat Resource Econ, Kingston, RI 02881 USA. [Fooks, Jacob R.] Econ Res Serv, USDA, Washington, DC USA. [Messer, Kent D.] Univ Delaware, Dept Appl Econ & Stat, Newark, DE USA. [Pradhanang, Soni M.] Univ Rhode Isl, Dept Geosci, Kingston, RI 02881 USA. [Suter, Jordan F.] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. RP Miao, HR (reprint author), Univ Rhode Isl, Dept Environm & Nat Resource Econ, Kingston, RI 02881 USA. EM haoran_miao@my.uri.edu FU National Science Foundation EPSCoR [IIA-1330406] FX This material is based upon work supported in part by the National Science Foundation EPSCoR Track-2 Cooperative Agreement IIA-1330406, Collaborative Research: North East Water Resources Network. The data used are listed in the references, tables, supplements. The authors declare that there is no conflict of interests regarding the publication of this paper. We are grateful to the comments from Scott C. Merrill, Arthur J. Gold and three reviewers. This article has also benefited from comments during presentations at 2015 summer conferences organized by Association of Environmental and Resource Economists and Northeastern Agricultural and Resource Economics Association. The views expressed are those of the authors and not necessarily those of the Economic Research Service or the U.S. Department of Agriculture. NR 35 TC 0 Z9 0 U1 5 U2 5 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD MAY PY 2016 VL 52 IS 5 BP 3294 EP 3308 DI 10.1002/2015WR018142 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DQ5QL UT WOS:000379259800002 ER PT J AU Sanchez-Martinez, G Moreno-Rico, O Smith, DR AF Sanchez-Martinez, Guillermo Moreno-Rico, Onesimo Smith, David R. TI FIRST RECORD AND BIOLOGICAL OBSERVATIONS ON THE WOODWASP Sirex obesus BRADLEY IN AGUASCALIENTES, MEXICO SO AGROCIENCIA LA English DT Article DE Siricidae; Sirex obesus; Pinus teocote; Pinus leiophylla; Ibalia leucospoides; Dendroctonus mexicanus ID UNITED-STATES; HYMENOPTERA; SIRICIDAE; SYMPHYTA AB The woodwasp Sirex obesus Bradley is described from specimens fortuitously captured in Lindgren funnel traps used in studies on the flight of the bark beetle Dendroctonus mexicanus Hopkins and through the direct capture on the bark of trees, in the municipality of San Jose de Gracia, Aguacalientes, Mexico. Pinus leiophylla Schl.et. Chain. and Pinus teocote Schl. et. Chain., weakened by the presence of D. mexicanus, were identified as hosts of S. obesus. The adults of this species emerge and oviposit during October and November. As parasitoids of S. obesus, some specimens of Ibalia leucospoides Hochenwarth (Hymenoptera: Ibaliidae) were collected in the same pine sections where the specimens of S. obesus emerged. This is the first record of the species in the state of Aguascalientes; therefore, more attention should be given to this insect because of its potential risk of invading pine trees and stands in Mexico. C1 [Sanchez-Martinez, Guillermo] INIFAP, Campo Expt Pabellon, Km 32-5,Carretera Aguascalientes Zacatecas, Pabellon De Arteaga 20670, Aguascalientes, Mexico. [Moreno-Rico, Onesimo] Univ Autonoma Aguascalientes, Ctr Ciencias Basicas, Dept Microbiol, Ave Univ 940,Colonia Ciudad Univ, Aguascalientes 20131, Aguascalientes, Mexico. [Smith, David R.] USDA, Systemat Entomol Lab, PSI, ARS,Natl Museum Nat Hist,Smithsonian Inst, POB 37012,MRC 168, Washington, DC 20013 USA. RP Sanchez-Martinez, G (reprint author), INIFAP, Campo Expt Pabellon, Km 32-5,Carretera Aguascalientes Zacatecas, Pabellon De Arteaga 20670, Aguascalientes, Mexico. EM omoreno@correo.uaa.mx; dave.smith@ars.usda.gov NR 14 TC 0 Z9 0 U1 2 U2 2 PU COLEGIO POSTGRADUADOS PI MONTECILLO PA CARRETERA MEXICO TEXCOCO KM 36 5, MONTECILLO 56230, ESTADO MEXICO, MEXICO SN 1405-3195 J9 AGROCIENCIA-MEXICO JI Agrociencia PD MAY-JUN PY 2016 VL 50 IS 4 BP 459 EP 469 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA DQ1IG UT WOS:000378954100007 ER PT J AU Kong, LY Baik, BK AF Kong, Lingyan Baik, Byung-Kee TI Degree of starchy endosperm separation from bran as a milling quality trait of wheat grain SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Bran; Flour yield; Wheat milling ID RED SPRING WHEAT; SOFT WHEAT; BAKING QUALITY; KERNEL SIZE; BREAD-BAKING; CULTIVARS; PERFORMANCE; MICROSCOPY; FLOUR; HPLC AB Easy and clean separation of starchy endosperm from bran during milling could have a significant contribution to the increased flour yield. Starch content and DMSO extractable content of bran were determined as the estimates for remnant endosperm content of bran for 61 and 100 soft red winter (SRW) wheat genotypes grown in 2013 and 2014, respectively. DMSO extractable content was found to be more reliable in the estimation of remnant endosperm content of bran than was starch content with better reproducibility. Flour yields of the 2013 and 2014 SRW wheat varieties tested ranged from 65.1 to 72.4% (w/w), and 63.6-73.9% (w/w), respectively. The remnant endosperm content of bran estimated using the DMSO extractable content ranged from 33.9 to 43.6% (w/w) and 35.4-47.4% (w/w) for the 2013 and 2014 crops, respectively. The remnant endosperm content was significantly related to flour yield with a correlation coefficient of r = -0.54 (P < 0.001) from the 2014 crop, which was greater than that for any other grain characteristics including test weight, kernel hardness, and kernel weight. The degree of endosperm separation varied with different wheat varieties, different bran pieces and even different parts of the same piece of bran. Published by Elsevier Ltd. C1 [Kong, Lingyan; Baik, Byung-Kee] ARS, USDA, Soft Wheat Qual Lab, Wooster, OH 44691 USA. RP Baik, BK (reprint author), ARS, USDA, Soft Wheat Qual Lab, Wooster, OH 44691 USA. EM byungkee.baik@ars.usda.gov FU Mondelez International Co. FX The authors would like to acknowledge the staff of the USDA-ARS Soft Wheat Quality Laboratory, Anne Sturbaum, Sharon Croskey, Tom Donelson, Scott Beil, Amy Bugaj and Tony Karcher, for their assistance in grain milling and quality tests, and Andrea Kaszas of the Molecular and Cellular Imaging Center at the Ohio Agricultural Research and Development Center for her assistance in the use of the microtome, fluorescence microscope and SEM. We gratefully acknowledge the financial support of the Mondelez International Co. NR 25 TC 0 Z9 0 U1 4 U2 5 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD MAY PY 2016 VL 69 BP 49 EP 56 DI 10.1016/j.jcs.2016.02.001 PG 8 WC Food Science & Technology SC Food Science & Technology GA DP2YO UT WOS:000378359700008 ER PT J AU Quayson, ET Atwell, W Morris, CF Marti, A AF Quayson, Enoch T. Atwell, William Morris, Craig F. Marti, Alessandra TI Empirical rheology and pasting properties of soft-textured durum wheat (Triticum turgidum ssp durum) and hard-textured common wheat (T. aestivum) SO JOURNAL OF CEREAL SCIENCE LA English DT Article DE Puroindolines; Kernel hardness; Gluten aggregation; Dough rheology ID DAMAGED STARCH; PUROINDOLINES; FLOUR; QUALITY; GLUTEN; L.; COMPONENTS; NOODLE AB Puroindoline (PIN) proteins are the molecular basis for wheat kernel texture classification and affect flour milling performance. This study investigated the effect of PINs on empirical rheology and pasting properties in Triticum turgidum ssp. durum and Triticum aestivum. Soft wheat (cv. Alpowa), durum wheat (cv. Svevo) and their derivatives in which PINs were deleted (Hard Alpowa) or expressed (cv. Soft Svevo). Presence of PINs affected flour particle size and damaged starch. PINs increased the pasting temperature and breakdown viscosity, while the effect on peak viscosity and setback were not consistent. Presence of PINs was negatively associated with GlutoPeak gluten aggregation energy and farinograph dough stability, suggesting a weakening of the gluten matrix. As regards dough extensibility, the role of PINs was evident only in common wheat: 5DS distal end deletion increased the resistance to extension, without affecting the dough extensibility. This study showed PINs to have different impact on pasting and rheological properties of T. aestivum and T. turgidum ssp. durum flours. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Quayson, Enoch T.; Atwell, William; Marti, Alessandra] Univ Minnesota, Dept Food Sci & Nutr, 1334 Eckles Ave, St Paul, MN 55108 USA. [Morris, Craig F.] Washington State Univ, USDA, ARS, Western Wheat Qual Lab,Food Sci & Human Nutr Faci, Pullman, WA 99164 USA. [Marti, Alessandra] Univ Milan, Dept Food Environm & Nutr Sci, Via G Celoria 2, I-20133 Milan, Italy. RP Marti, A (reprint author), Univ Minnesota, Dept Food Sci & Nutr, 1334 Eckles Ave, St Paul, MN 55108 USA. EM amarti@umn.edu NR 30 TC 1 Z9 1 U1 17 U2 19 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0733-5210 EI 1095-9963 J9 J CEREAL SCI JI J. Cereal Sci. PD MAY PY 2016 VL 69 BP 252 EP 258 DI 10.1016/j.jcs.2016.03.017 PG 7 WC Food Science & Technology SC Food Science & Technology GA DP2YO UT WOS:000378359700036 ER PT J AU Pureswaran, DS Hofstetter, RW Sullivan, BT Grady, AM Brownie, C AF Pureswaran, Deepa S. Hofstetter, Richard W. Sullivan, Brian T. Grady, Amanda M. Brownie, Cavell TI Western Pine Beetle Populations in Arizona and California Differ in the Composition of Their Aggregation Pheromones SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Western pine beetle; Southern pine beetle; Interspecific attraction; Geographic variation; Pheromone; Character displacement; Scolytinae ID DENDROCTONUS-BREVICOMIS COLEOPTERA; BARK-BEETLES; FRONTALIS COLEOPTERA; CHEMICAL COMMUNICATION; HOST COLONIZATION; EXO-BREVICOMIN; FIELD RESPONSE; PONDEROSA PINE; SCOLYTIDAE; CURCULIONIDAE AB We compared pheromone production and response for populations of western pine beetle, Dendroctonus brevicomis LeConte, from sites in northern Arizona and northern California. Volatiles were collected from individuals of both sexes that had mined as a pair in a Pinus ponderosa log for 1 d, and they were subsequently analyzed by gas chromatography coupled to mass-spectrometry. Principal component analysis of quantities of Dendroctonus pheromone components indicated strong site-associated clustering of blend composition for females but not males. Much of the clustering in females evidently was due to differences in the production of endo- and exo-brevicomin, which occurred in average ratios of 0.1:1 and 19:1 for populations in the California and Arizona sites, respectively. In the California site, exo- was better than endo-brevicomin in enhancing trap catches of both sexes to lures containing the host-tree odor alpha-pinene and the male-produced aggregation pheromone component frontalin. In an identical test in the Arizona site, endo- was a better adjuvant than exo-brevicomin for male attraction, whereas females did not show a significant preference. At neither location were the isomers antagonistic to one another in activity. Thus, one aggregation pheromone has apparently diverged between these populations, concurrent with published evidence that D. brevicomis on either side of the Great Basin are genetically distinct and are possibly different species. Furthermore, production of and response to the isomers of brevicomin by flying Dendroctonus frontalis Zimmermann in the Arizona site were similar to those of sympatric D. brevicomis. This interspecific signal overlap is likely sustainable since joint species mass-attacks may assist both species in overcoming host defenses, thereby increasing host availability. C1 [Pureswaran, Deepa S.] Laurentian Forestry Ctr, Canadian Forest Serv, 1055 Rue PEPS, Quebec City, PQ G1V 4C7, Canada. [Hofstetter, Richard W.] No Arizona Univ, Sch Forestry, Box 15018, Flagstaff, AZ 86011 USA. [Sullivan, Brian T.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. [Grady, Amanda M.] US Forest Serv, USDA, Forest Hlth Protect, AZ Zone, Flagstaff, AZ 86001 USA. [Brownie, Cavell] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. RP Pureswaran, DS (reprint author), Laurentian Forestry Ctr, Canadian Forest Serv, 1055 Rue PEPS, Quebec City, PQ G1V 4C7, Canada. EM deepa.pureswaran@canada.ca FU Rocky Mountain Research Station JV aggreement [08-JV-11221633-250]; Canadian Forest Service FX We thank Joanne Barrett, Eli Jensen, Seth Davis, and Chris Foelker for trap collection, identification, and data entry. We thank Daniel Cluck for assistance with collection of infested material in California. Rob Johns and Kathy Bleiker provided comments on an early draft. Research was funded by Rocky Mountain Research Station JV aggreement 08-JV-11221633-250 with R.W.H and an operating grant to D.S.P. from the Canadian Forest Service. NR 58 TC 1 Z9 1 U1 4 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD MAY PY 2016 VL 42 IS 5 BP 404 EP 413 DI 10.1007/s10886-016-0696-9 PG 10 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DP9LS UT WOS:000378818300005 PM 27125814 ER PT J AU Choi, MY Ahn, SJ Park, KC Vander Meer, R Carde, RT Jurenka, R AF Choi, Man-Yeon Ahn, Seung-Joon Park, Kye-Chung Vander Meer, Robert Carde, Ring T. Jurenka, Russell TI Tarsi of Male Heliothine Moths Contain Aldehydes and Butyrate Esters as Potential Pheromone Components SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Hexadecanal; Octadecanal; Butyrate esters; Heliothine moths; Male pheromones ID FEMALE SEX-PHEROMONE; ASSULTA GUENEE LEPIDOPTERA; OLFACTORY RECEPTOR NEURONS; ORIENTAL TOBACCO BUDWORM; VIRESCENS LEPIDOPTERA; HELICOVERPA-ASSULTA; FRUIT MOTHS; NOCTUIDAE; IDENTIFICATION; ZEA AB The Noctuidae are one of the most speciose moth families and include the genera Helicoverpa and Heliothis. Females use (Z)-11-hexadecenal as the major component of their sex pheromones except for Helicoverpa assulta and Helicoverpa gelotopoeon, both of which utilize (Z)-9-hexadecenal. The minor compounds found in heliothine sex pheromone glands vary with species, but hexadecanal has been found in the pheromone gland of almost all heliothine females so far investigated. In this study, we found a large amount (0.5-1.5 mu g) of hexadecanal and octadecanal on the legs of males of four heliothine species, Helicoverpa zea, Helicoverpa armigera, H. assulta, and Heliothis virescens. The hexadecanal was found on and released from the tarsi, and was in much lower levels or not detected on the remaining parts of the leg (tibia, femur, trochanter, and coxa). Lower amounts (0.05-0.5 mu g) of hexadecanal were found on female tarsi. This is the first known sex pheromone compound to be identified from the legs of nocturnal moths. Large amounts of butyrate esters (about 16 mu g) also were found on tarsi of males with lower amounts on female tarsi. Males deposited the butyrate esters while walking on a glass surface. Decapitation did not reduce the levels of hexadecanal on the tarsi of H. zea males, indicating that hexadecanal production is not under the same neuroendocrine regulation system as the production of female sex pheromone. Based on electroantennogram studies, female antennae had a relatively high response to hexadecanal compared to male antennae. We consider the possible role of aldehydes and butyrate esters as courtship signals in heliothine moths. C1 [Choi, Man-Yeon] ARS, USDA, Hort Crops Res Lab, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. [Ahn, Seung-Joon] Max Planck Inst Chem Ecol, Dept Entomol, D-07745 Jena, Germany. [Park, Kye-Chung] Hort & Food Res Inst, Gerald St, Lincoln 8152, New Zealand. [Vander Meer, Robert] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Carde, Ring T.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Jurenka, Russell] Iowa State Univ, Dept Entomol, Sci 2 434, Ames, IA 50011 USA. RP Jurenka, R (reprint author), Iowa State Univ, Dept Entomol, Sci 2 434, Ames, IA 50011 USA. EM rjurenka@iastate.edu OI Ahn, Seung-Joon/0000-0002-5980-106X FU BARD, the United States-Israel Binational Agricultural Research and Development Fund [IS-4722-14] FX We thank the late Dr. Peter Teal for providing 16:Ald, Anne Barrington for supplying H. armigera for the EAG study, and Drs. CY Yang and JK Jung for technical help. A portion of this study was funded by Research Grant IS-4722-14 from BARD, the United States-Israel Binational Agricultural Research and Development Fund to RJ. NR 41 TC 1 Z9 1 U1 3 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 EI 1573-1561 J9 J CHEM ECOL JI J. Chem. Ecol. PD MAY PY 2016 VL 42 IS 5 BP 425 EP 432 DI 10.1007/s10886-016-0701-3 PG 8 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA DP9LS UT WOS:000378818300007 PM 27155602 ER PT J AU Hwang, HS Singh, M Lee, S AF Hwang, Hong-Sik Singh, Mukti Lee, Suyong TI Properties of Cookies Made with Natural Wax-Vegetable Oil Organogels SO JOURNAL OF FOOD SCIENCE LA English DT Article DE cookie; margarine; organogel; vegetable oil; wax ID CANDELILLA WAX; BAKING PERFORMANCE; SUNFLOWER WAX; SATURATED FAT; CARNAUBA WAX; RATS; BEHAVIOR; BEESWAX; DISEASE; LIVER AB The aim of this study was to examine the feasibility of cookies in which the conventional margarine is replaced with an organogel of vegetable oil (VO) and natural wax. New cookies from VO organogels contain no trans fats and much less saturated fats than cookies made with a conventional margarine. To understand the effects of different kinds of waxes, organogels were prepared from 4 different waxes including sunflower wax (SW), rice bran wax (RBW), beeswax, and candelilla wax and properties of cookie dough and cookie were evaluated. To investigate the effects of different VOs on the properties of cookies, 3 VOs including olive oil, soybean oil and flaxseed oil representing oils rich in oleic acid (18:1), linoleic acid (18:2), and linolenic acid (18:3), respectively, were used. Both the wax and VO significantly affected properties of organogel such as firmness and melting behavior shown in differential scanning calorimetry. The highest firmness of organogel was observed with SW and flaxseed oil. Properties of dough such as hardness and melting behavior were also significantly affected by wax and VO while trends were somewhat different from those for organogels. SW and RBW provided greatest hardnesses to cookie dough. However, hardness, spread factor, and fracturability of cookie containing the wax-VO organogel were not significantly affected by different waxes and VOs. Several cookies made with wax-VO organogels showed similar properties to cookies made with a commercial margarine. Therefore, this study shows the high feasibility of utilization of the organogel technology in real foods such as cookies rich in unsaturated fats. C1 [Hwang, Hong-Sik; Singh, Mukti] ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, 1815 N Univ St, Peoria, IL 61604 USA. [Lee, Suyong] Sejong Univ, Dept Food Sci & Technol, 98 Gunja Dong, Seoul 143747, South Korea. [Lee, Suyong] Sejong Univ, Carbohydrate Bioprod Res Ctr, 98 Gunja Dong, Seoul 143747, South Korea. RP Hwang, HS (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Funct Foods Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Hongsik.hwang@ars.usda.gov NR 35 TC 0 Z9 0 U1 18 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD MAY PY 2016 VL 81 IS 5 BP C1045 EP C1054 DI 10.1111/1750-3841.13279 PG 10 WC Food Science & Technology SC Food Science & Technology GA DQ0UN UT WOS:000378917300001 PM 27027545 ER PT J AU Benjankar, R Tonina, D Marzadri, A McKean, J Isaak, DJ AF Benjankar, Rohan Tonina, Daniele Marzadri, Alessandra McKean, Jim Isaak, Daniel J. TI Effects of habitat quality and ambient hyporheic flows on salmon spawning site selection SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE salmonid species; spawning habitat; redd; habitat quality; surface water curvature; hyporheic flow ID FALL CHINOOK SALMON; EGG BURIAL DEPTHS; REDD-SITE; SALVELINUS-FONTINALIS; EMBRYO SURVIVAL; MOKELUMNE RIVER; COLUMBIA RIVER; BROOK TROUT; EXCHANGE; STREAM AB Understanding the role of stream hydrologic and morphologic variables on the selection of spawning sites by salmonid fishes at high resolution across broad scales is needed for effective habitat restoration and protection. Here we used remotely sensed meter-scale channel bathymetry for a 13.5km reach of Chinook salmon spawning stream in central Idaho to describe habitat quality and set boundary conditions for a two-dimensional surface water model coupled with a three-dimensional hyporheic flux model. Metrics describing ambient hyporheic flow intensity and habitat quality, which is quantified as a function of stream hydraulics and morphology, were compared to the locations of nests built by female salmon. Nest locations were predicted most accurately by habitat quality followed by channel morphology (i.e., riffles location). As a lesser degree than habitat quality, water surface curvature was also a good indicator of spawning location because its intensity can identify riffle morphology. The ambient hyporheic flow predicted at meter-scale resolution was not a strong predictor of redd site selection. Furthermore, the study suggests direct morphological measurements obtained from easily measured channel bathymetry data could enable effective and rapid assessments of salmon spawning channels across broad areas. C1 [Benjankar, Rohan; Tonina, Daniele] Univ Idaho, Ctr Ecohydraul Res, Boise, ID 83706 USA. [Marzadri, Alessandra] Univ Trent, Dept Civil Environm & Mech Engn, Trento, Italy. [McKean, Jim; Isaak, Daniel J.] US Forest Serv, Rocky Mt Res Stn, Boise, ID USA. RP Benjankar, R (reprint author), Univ Idaho, Ctr Ecohydraul Res, Boise, ID 83706 USA. EM rohanb@uidaho.edu RI Tonina, Daniele/I-7688-2012 OI Tonina, Daniele/0000-0002-1866-1013 FU Center for Ecohydraulics Research (CER); University of Idaho (UI) Research Office; U.S. Forest Service; NSF [1344602] FX The Center for Ecohydraulics Research (CER) and the University of Idaho (UI) Research Office have supported this research through providing partial support for the first author. Partial support is provided by the U.S. Forest Service and NSF grant 1344602. We thank the Editors, Jud Harvey, and three anonymous reviewers for their constructive comments on an earlier version. Their suggestions greatly improved the manuscript. The data used in this study are available by request to the corresponding author. We acknowledge the Danish Hydraulic Institute (DHI) for providing MIKE software packages to the University of Idaho. Any opinions, conclusions, or recommendations expressed in this article are those of the authors and do not necessarily reflect the views of supporting institutions. NR 114 TC 0 Z9 0 U1 8 U2 12 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD MAY PY 2016 VL 121 IS 5 BP 1222 EP 1235 DI 10.1002/2015JG003079 PG 14 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DP7TX UT WOS:000378703200001 ER PT J AU Argerich, A Haggerty, R Johnson, SL Wondzell, SM Dosch, N Corson-Rikert, H Ashkenas, LR Pennington, R Thomas, CK AF Argerich, Alba Haggerty, Roy Johnson, Sherri L. Wondzell, Steven M. Dosch, Nicholas Corson-Rikert, Hayley Ashkenas, Linda R. Pennington, Robert Thomas, Christoph K. TI Comprehensive multiyear carbon budget of a temperate headwater stream SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE carbon; biogeochemistry; stream; carbon fluxes; evasion ID DISSOLVED ORGANIC-CARBON; OXYGEN CHANGE TECHNIQUE; ECOSYSTEM METABOLISM; HYPORHEIC ZONE; INLAND WATERS; DIOXIDE; CATCHMENTS; DYNAMICS; RIVERS; FLUXES AB Headwater streams comprise nearly 90% of the total length of perennial channels in global catchments. They mineralize organic carbon entering from terrestrial systems, evade terrestrial carbon dioxide (CO2), and generate and remove carbon through in-stream primary production and respiration. Despite their importance, headwater streams are often neglected in global carbon budgets primarily because of a lack of available data. We measured these processes, in detail, over a 10year period in a stream draining a 96ha forested watershed in western Oregon, USA. This stream, which represents only 0.4% of the watershed area, exported 159kgCha(-1)yr(-1), similar to the global exports for large rivers. Stream export was dominated by downstream transport of dissolved inorganic carbon (63kgCha(-1)yr(-1)) and by evasion of CO2 to the atmosphere (42kgCha(-1)yr(-1)), leaving the remainder of 51kgCha(-1)yr(-1) for downstream transport of organic carbon (17kgCha(-1)yr(-1) and 34kgCha(-1)yr(-1) in dissolved and particulate form, respectively). C1 [Argerich, Alba] Oregon State Univ, Coll Forestry, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Haggerty, Roy; Dosch, Nicholas; Corson-Rikert, Hayley; Pennington, Robert; Thomas, Christoph K.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Johnson, Sherri L.; Wondzell, Steven M.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Ashkenas, Linda R.] Oregon State Univ, Coll Agr Sci, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Thomas, Christoph K.] Univ Bayreuth, Micrometeorol Grp, Bayreuth, Germany. RP Argerich, A (reprint author), Oregon State Univ, Coll Forestry, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. EM alba.argerich@oregonstate.edu RI Haggerty, Roy/A-5863-2009 FU HJ Andrews Experimental Forest research program; National Science Foundation's Long-Term Ecological Research Program [DEB 08-23380]; U.S. Forest Service Pacific Northwest Research Station,; Oregon State University; NSF [EAR 14-17603, AGS0955444]; OSU Hollis Dole Fund for Environmental Geology; U.S. Forest Service Pacific Northwest Research Station [10-JV-11261991-055] FX Access to all data sets used for this study are available through the H.J. Andrews Experimental Forest data repository (http://andrewsforest.oregonstate.edu/lter/data.cfm?frameURL=8). Funding for A. A., long-term data, and facilities were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 08-23380), U.S. Forest Service Pacific Northwest Research Station, and Oregon State University. Parts of this study were funded by NSF grants EAR 14-17603 and AGS0955444, the OSU Hollis Dole Fund for Environmental Geology, and the U.S. Forest Service Pacific Northwest Research Station (Joint Venture Agreement 10-JV-11261991-055). NR 57 TC 1 Z9 1 U1 5 U2 14 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD MAY PY 2016 VL 121 IS 5 BP 1306 EP 1315 DI 10.1002/2015JG003050 PG 10 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DP7TX UT WOS:000378703200007 ER PT J AU Tingley, MW Wilkerson, RL Howell, CA Siegel, RB AF Tingley, Morgan W. Wilkerson, Robert L. Howell, Christine A. Siegel, Rodney B. TI An integrated occupancy and space-usemodel to predict abundance of imperfectly detected, territorial vertebrates SO METHODS IN ECOLOGY AND EVOLUTION LA English DT Article DE Bayesian; Black-backed Woodpecker; density; home range; Picoides arcticus; population size; wildlife habitat model ID BLACK-BACKED WOODPECKERS; HOME-RANGE SIZE; HABITAT; MODELS; DENSITY; POPULATIONS; HUMMINGBIRDS; COMPETITION; TELEMETRY; DYNAMICS AB 1. It is often highly desirable to know not only where species are likely to occur (i.e. occupancy) but also how many individuals are supported by a given habitat (i.e. density). For many animals, occupancy and density may be determined by distinct ecological processes. 2. Here we develop a novel abundance model as the product of landscape-scale occupancy probability and habitat-scale density given occupancy. One can conceptualize our model as fully packing a landscape with home ranges or territories based on habitat quality, and then subtracting territories based on a probabilistic process that accounts for the fact that species rarely exhibit full occupancy across heterogeneous landscapes. The model is designed to predict abundance at fine spatial scales, using resolutions equal to or smaller than a single home range or territory. 3. We demonstrate this model on the Black-backed Woodpecker (Picoides arcticus), a species of management concern linked to post-fire forests. Occupancy is derived from a regional monitoring effort, while density given occupancy comes from a telemetry study of variation in territory size. A Bayesian framework is used to combine independent occupancy and home-range size models and predict abundance of Black-backed Woodpeckers at 4 fires that burned in 2012 or 2013. Predictions are evaluated with independently collected survey data, showing that the model is successful at predicting both absolute abundance at fires as well as relative abundance within and among fires. 4. The conceptual model presents a promising new framework for fine-scale modelling of density and abundance for other territorial yet elusive species. Telemetry and occupancy data are widely collected for many species, but rarely utilized in combination, and the ecological exploration of the factors that determine occurrence versus home-range size may provide useful biological insight. As applied to the Black-backed Woodpecker, the model provides a tool for resource managers to explore trade-offs in retaining burned forest habitat versus managing for other post-fire goals, such as salvage logging or reforestation efforts that require snag removal. C1 [Tingley, Morgan W.] Univ Connecticut, Ecol & Evolutionary Biol, 75 N Eagleville Rd,Unit 3043, Storrs, CT 06269 USA. [Tingley, Morgan W.; Wilkerson, Robert L.; Siegel, Rodney B.] Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. [Howell, Christine A.] US Forest Serv, USDA, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. RP Tingley, MW (reprint author), Univ Connecticut, Ecol & Evolutionary Biol, 75 N Eagleville Rd,Unit 3043, Storrs, CT 06269 USA.; Tingley, MW (reprint author), Inst Bird Populat, POB 1346, Point Reyes Stn, CA 94956 USA. EM morgan.tingley@uconn.edu OI Tingley, Morgan/0000-0002-1477-2218 NR 46 TC 2 Z9 2 U1 7 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2041-210X EI 2041-2096 J9 METHODS ECOL EVOL JI Methods Ecol. Evol. PD MAY PY 2016 VL 7 IS 5 BP 508 EP 517 DI 10.1111/2041-210X.12500 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DP8FC UT WOS:000378732500001 ER PT J AU Sollmann, R Gardner, B Williams, KA Gilbert, AT Veit, RR AF Sollmann, Rahel Gardner, Beth Williams, Kathryn A. Gilbert, Andrew T. Veit, Richard R. TI A hierarchical distance sampling model to estimate abundance and covariate associations of species and communities SO METHODS IN ECOLOGY AND EVOLUTION LA English DT Article DE Bayesian P-value; cluster size; hierarchical model; seabirds; sparse data; wildlife surveys ID BREEDING BIRDS; COUNT DATA; RICHNESS; SEABIRDS; DENSITY; SYSTEM; BIAS; SEA AB 1. Distance sampling is a common survey method in wildlife studies, because it allows accounting for imperfect detection. The framework has been extended to hierarchical distance sampling (HDS), which accommodates the modelling of abundance as a function of covariates, but rare and elusive species may not yield enough observations to fit such a model. 2. We integrate HDS into a community modelling framework that accommodates multi-species spatially replicated distance sampling data. The model allows species-specific parameters, but these come from a common underlying distribution. This form of information sharing enables estimation of parameters for species with sparse data sets that would otherwise be discarded from analysis. We evaluate the performance of the model under varying community sizes with different species-specific abundances through a simulation study. We further fit the model to a seabird data set obtained from shipboard distance sampling surveys off the East Coast of the USA. 3. Comparing communities comprised of 5, 15 or 30 species, bias of all community-level parameters and some species-level parameters decreased with increasing community size, while precision increased. Most species-level parameters were less biased for more abundant species. For larger communities, the community model increased precision in abundance estimates of rarely observed species when compared to single-species models. For the seabird application, we found a strong negative association of community and species abundance with distance to shore. Water temperature and prey density had weak effects on seabird abundance. Patterns in overall abundance were consistent with known seabird ecology. 4. The community distance sampling model can be expanded to account for imperfect availability, imperfect species identification or other missing individual covariates. The model allowed us to make inference about ecology of species communities, including rarely observed species, which is particularly important in conservation and management. The approach holds great potential to improve inference on species communities that can be surveyed with distance sampling. C1 [Sollmann, Rahel; Gardner, Beth] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Williams, Kathryn A.; Gilbert, Andrew T.] Biodivers Res Inst, Gorham, ME 04038 USA. [Veit, Richard R.] CUNY Coll Staten Isl, Div Humanities & Social Sci, New York, NY 10314 USA. [Sollmann, Rahel] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. RP Sollmann, R (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. EM rsollmann@ucdavis.edu FU Department of Energy's Office of Energy Efficiency & Renewable Energy [DE-EE0005362] FX We thank Holly Goyert, Nicholas Flanders, David L. Miller, Paul Conn and three anonymous reviewers for helpful comments on an earlier draft of this manuscript. Data were collected by Christopher Vogel, Holly Goyert and Emily Connelly, with support from boat captain Brian Patteson. This material is based upon work supported by the Department of Energy's Office of Energy Efficiency & Renewable Energy under Award Number DE-EE0005362. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favouring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. NR 42 TC 0 Z9 0 U1 17 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2041-210X EI 2041-2096 J9 METHODS ECOL EVOL JI Methods Ecol. Evol. PD MAY PY 2016 VL 7 IS 5 BP 529 EP 537 DI 10.1111/2041-210X.12518 PG 9 WC Ecology SC Environmental Sciences & Ecology GA DP8FC UT WOS:000378732500003 ER PT J AU Zheng, ZH Sharratt, B Feng, G Li, XH Pi, HW AF Zheng, Zehao Sharratt, Brenton Feng, Gary Li, Xinhu Pi, Huawei TI Wind Erosion of Cropland in the Northwestern Tarim Basin SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID COLUMBIA PLATEAU; DUST-STORM; VERTICAL-DISTRIBUTION; AGRICULTURAL FIELDS; WINDBLOWN SEDIMENT; TAKLIMAKAN DESERT; ERODED SEDIMENT; SURFACE WIND; SOIL; SIZE AB The Aksu region within the Tarim Basin is a major source of windblown dust in China due to the arid climate and vast areas under intensive irrigated crop production. Despite the importance of crop production to human subsistence and the local economy, little is known about the amount of soil eroded by wind from agricultural lands in the region. Passive aeolian sediment collectors were used to measure soil loss from an irrigated cotton (Gossypium spp.) field and red date (Ziziphus jujuba Mill.) orchard in the Aksu Oasis during high wind events in 2012 and 2013. Mass of sediment trapped by the collectors was best described as a power function of height above the surface in the cotton field and red date orchard. Soil loss from the cotton field ranged from 0 to 142 g m(-2), whereas soil loss from the red date orchard ranged from 0 to 71 g m(-2) for single- or multiple-day high wind events. Total soil loss from the cotton field and red date orchard, for the high wind events observed in this study, was 130 and 96 g m(-2), respectively, in 2012 and 305 and 165 g m(-2), respectively, in 2013. Soil loss was similar to that reported from agricultural lands in other regions of the world. Although soil loss is relatively small compared with the Gobi Desert in China, poor air quality in the Tarim Basin necessitates identifying crop and soil management practices for reducing windblown dust and conserving the soil resource. C1 [Zheng, Zehao] Xinjiang Inst Ecol & Geog, Urumqi, Xinjiang, Peoples R China. [Sharratt, Brenton] Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. [Feng, Gary] USDA ARS, Crop Sci Res Lab, POB 5367,810 Highway 12E, Mississippi State, MS 39762 USA. [Li, Xinhu; Pi, Huawei] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, 818 Beijing South Rd, Urumqi, Xinjiang, Peoples R China. RP Sharratt, B (reprint author), Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. EM brenton.sharratt@ars.usda.gov FU National Natural Science Foundation of China [41171019]; One Hundred Talented Researchers Program of Chinese Academy of Sciences FX This research was supported by the National Natural Science Foundation of China (Grant no. 41171019) and One Hundred Talented Researchers Program of Chinese Academy of Sciences. NR 52 TC 0 Z9 0 U1 2 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 672 EP 682 DI 10.2136/sssaj2015.07.0259 PG 11 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900013 ER PT J AU Wang, J Watts, DB Meng, QQ Zhang, QF Way, TR AF Wang, Jian Watts, Dexter B. Meng, Qinqian Zhang, Qingfeng Way, Thomas R. TI Influence of Surface Crusting on Infiltration of a Loess Plateau Soil SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID TYPICAL CHINESE SOILS; SIMULATED RAINFALL; LAYERED SOILS; EROSION; MANAGEMENT; RUNOFF; EFFICIENCY; INTENSITY; EMERGENCE; TILLAGE AB Crusting frequently occurs on farmland soil of the Loess Plateau in China and often adversely affects agricultural yields. Therefore, a study was conducted to evaluate the occurrence of soil crusting and its influence on infiltration in a Loess Plateau silt loam soil. Simulated rainfall events were conducted at two intensities (0.67 and 1.33 mm min(-1)) on four slopes (5, 9, 18, and 29%) under two soil conditions (crusted and uncrusted). Infiltration was evaluated as the difference between precipitation applied and water lost to runoff. A modified Green-Ampt model adjusted to account for soil crusting was also evaluated for predicting infiltration of crusted soil. Mean soil crust thickness was 3.33 and 3.94 mm under rainfall intensities of 0.67 and 1.33 mm min(-1), respectively. Slope had no effect on crust formation. In all instances, cumulative infiltration was lower in crusted soil than in uncrusted soil. The time to runoff decreased from 17.98 min in uncrusted soil to 4.64 min in crusted soil under a rainfall intensity of 0.67 mm min(-1). Time to runoff also decreased from 16.25 min in uncrusted soil to 4.54 min in crusted soil at a rainfall intensity of 1.33 mm min(-1). The modified Green-Ampt model provided a good estimate of soil crusting's influence on infiltration (coefficient of determination range 0.85-0.97). This suggests that the Green-Ampt model could be used as a tool for predicting soil crusting's influence on infiltration and runoff from soils of the Loess Plateau. C1 [Wang, Jian; Zhang, Qingfeng] Northwest A&F Univ, Coll Nat Resource & Environm, Yangling 712100, Shaanxi, Peoples R China. [Watts, Dexter B.; Way, Thomas R.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Meng, Qinqian] Northwest A&F Univ, Coll Water Resources & Architectural Engn, Yangling 712100, Shaanxi, Peoples R China. RP Wang, J (reprint author), Northwest A&F Univ, Coll Nat Resource & Environm, Yangling 712100, Shaanxi, Peoples R China. EM wangjian@nwsuaf.edu.cn NR 46 TC 0 Z9 0 U1 14 U2 14 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 683 EP 692 DI 10.2136/sssaj2015.08.0291 PG 10 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900014 ER PT J AU Sharratt, B Schillinger, WF AF Sharratt, Brenton Schillinger, William F. TI Soil Characteristics and Wind Erosion Potential of Wheat-Oilseed-Fallow Cropping Systems SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID COLUMBIA PLATEAU; DECOMPOSITION; TILLAGE; AGGREGATION; RESIDUES; COVER; CROPS AB Oilseeds are integral to the production of biofuels and diversifying rainfed cropping systems in the US Pacific Northwest (PNW). However, there is evidence to suggest greater potential for wind erosion when growing oilseeds in wheat (Triticum aestivum L.) rotations. Little is known concerning the impact of growing oilseeds on the soil surface characteristics that affect erosion. Soil characteristics were examined during the fallow phase of three crop rotations: (i) winter wheat-summer fallow (WW-SF), (ii) winter wheat-camelina (Camelina sativa L. Grantz)-summer fallow (WW-C-SF), and (iii) winter wheat-safflower (Carthamus tinctorius L.)-summer fallow (WW-S-SF) at Lind and Ritzville, WA. Crop residue biomass and soil water content, roughness, surface strength, and aggregate size distribution were measured immediately after sowing winter wheat. Camelina and safflower did not affect soil water content, random roughness, penetration resistance, geometric mean diameter, or the erodible fraction, possibly due to the short duration over which the rotations were monitored in this study. Flat residue biomass and cover, however, tended to be greater in the WW-SF rotation. The Revised Wind Erosion Equation suggested that sediment transport could be from 57 to 212% greater for the WW-C-SF or WW-S-SF than the WW-SF rotation due to differences in crop residue characteristics after sowing wheat. These results indicate that crop residue must be carefully managed to minimize the occurrence and intensity of wind erosion from dryland oil-seed cropping systems in the PNW. Specifically, no-till management may be required to manage crop residues during the fallow phase of a wheat-oil-seed-fallow rotation to control wind erosion. C1 [Sharratt, Brenton] Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. [Schillinger, William F.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Sharratt, B (reprint author), Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. EM sharratt@wsu.edu NR 22 TC 0 Z9 0 U1 14 U2 17 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 704 EP 710 DI 10.2136/sssaj2015.12.0427 PG 7 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900016 ER PT J AU Tormena, CA Karlen, DL Logsdon, S Cherubin, MR AF Tormena, Cassio A. Karlen, Douglas L. Logsdon, Sally Cherubin, Mauricio R. TI Visual Soil Structure Effects of Tillage and Corn Stover Harvest in Iowa SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID RESIDUE REMOVAL; GRASSLAND MANAGEMENT; NO-TILLAGE; HYDRAULIC-PROPERTIES; PHYSICAL-PROPERTIES; CROP RESPONSE; COVER CROP; QUALITY; IMPACTS; ROTATION AB High levels of corn (Zea mays L.) stover harvest for ethanol production have raised concerns regarding negative consequences on soil structure and physical quality. Visual soil structure assessment methods have the potential to help address these concerns through simple, straightforward on-farm evaluations. Our objective was to determine if the visual evaluation of soil structure (VESS) approach could detect soil structural quality differences associated with different levels of corn stover harvest and tillage practices. We evaluated no harvest and moderate and high stover harvest levels within no-tillage (NT) and chisel plow (CP) plots following 7 yr of continuous corn. Undisturbed 10- by 20- by 25-cm soil samples were taken using a spade in April 2015. The thickness and structural quality (Sq) scores for the 0- to 5- (top) and 5- to 20-cm (bottom) soil layers were determined, and an overall Sq was computed. The Sq values showed a significant interaction between corn stover harvest and tillage practice. Soil structural quality showed no significant differences between NT and CP systems for either the no-or moderate-harvest treatments, but with a high stover harvest rate, CP structural quality was worse than NT. With NT, both moderate and high rates of stover harvest significantly increased Sq compared to no harvest; in CP, there was no significant difference between the no-and moderate-harvest treatments. Only a high level of stover harvest had an adverse effect on the Sq rating. The VESS approach was sensitive for detecting the effects of corn stover harvest and tillage systems on soil structural quality and should be further evaluated as an integrative, on-farm soil quality/health indicator. C1 [Tormena, Cassio A.] Univ Estadual Maringa, 5790 Colombo Ave, BR-87020900 Maringa, PR, Brazil. [Karlen, Douglas L.; Logsdon, Sally] USDA ARS, Natl Lab Agr & Environ, 2110 Univ Blvd, Ames, IA 50011 USA. [Cherubin, Mauricio R.] Univ Sao Paulo, Luiz de Queiroz Coll Agr, 11 Padua Dias Ave, BR-13418900 Piracicaba, SP, Brazil. RP Tormena, CA (reprint author), Univ Estadual Maringa, 5790 Colombo Ave, BR-87020900 Maringa, PR, Brazil. EM cassiotormena@gmail.com FU National Council for Scientific and Technological Development (CNPq), Brazil at the National Laboratory for Agriculture and the Environment, USDA/ARS, Ames, IA [203376/2014-5]; Sao Paulo Research Foundation (FAPESP) [2013/17581-6, 2014/16612-8] FX C.A. Tormena thanks the National Council for Scientific and Technological Development (CNPq), Brazil for the scholarship (process 203376/2014-5) granted as a visiting scientist at the National Laboratory for Agriculture and the Environment, USDA/ARS, Ames, IA. M.R. Cherubin thanks the Sao Paulo Research Foundation (FAPESP) for research grants (processes 2013/17581-6 and 2014/16612-8). NR 48 TC 0 Z9 0 U1 6 U2 8 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 720 EP 726 DI 10.2136/sssaj2015.12.0425 PG 7 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900018 ER PT J AU Jang, W Page-Dumroese, DS Keyes, CR AF Jang, Woongsoon Page-Dumroese, Deborah S. Keyes, Christopher R. TI Long-Term Soil Changes from Forest Harvesting and Residue Management in the Northern Rocky Mountains SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER; WHOLE-TREE; TEMPERATE FORESTS; UNITED-STATES; NUTRIENT DISTRIBUTION; SITE PRODUCTIVITY; BRITISH-COLUMBIA; WESTERN MONTANA; BIOMASS REMOVAL; CARBON AB Soil changes associated with forest harvesting, differing utilization levels, and post-harvest prescribed burning were determined using an empirical study to investigate the long-term impacts on soil physical and chemical properties at Coram Experimental Forest in northwestern Montana. In 1974, two replications of three regeneration cuttings (shelterwood, group selection, and clearcut) were installed. In addition, four residue management regimes (high utilization with no burning, medium with no burning, medium with broadcast burning, and low with broadcast burning) were implemented (similar to 74, 63, 65, and 54% wood removal, respectively). Thirty-eight years after harvesting, changes were evaluated in mineral soil and forest floor physical and chemical properties (organic matter [OM], C, N, Ca, K, and Mg pools, soil bulk density, and pH) and in coarse woody debris levels. There were no differences in soil pH and bulk density across all regeneration cuttings and residue treatments, probably due to the minimal soil effects associated with the forest harvesting operations that were used (hand felling and cable yarding). Comparisons between harvest and burning and the control indicate no statistical differences in OM, C, and N contents. Minor differences in extractable cation pools were noted in several comparisons among the treatments; these may be attributed to litter inputs from the differing vegetation compositions of over-story and shrub layers rather than nutrient changes within the mineral soil itself. At this moist-cool forest, intensive biomass utilization, with or without broadcast burning, had few long-term impacts on soil properties of soil C, OM, and nutrients. C1 [Jang, Woongsoon; Keyes, Christopher R.] Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. [Page-Dumroese, Deborah S.] US Forest Serv, Rocky Mt Res Stn, 1221 South Main, Moscow, ID 83843 USA. RP Jang, W (reprint author), Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. EM woongsoon.jang@umontana.edu FU Agriculture and Food Research Initiative, Biomass Research and Development Initiative, Competitive from the USDA National Institute of Food and Agriculture [2010-05325] FX This was a study of the Applied Forest Management Program at the University of Montana, a research and outreach unit of the Montana Forest and Conservation Experiment Station. We are grateful to T. Perry, J. Tirocke, J. Crotteau, D. Wright, E. Kennedy-Sutherland, and M. Jurgensen for their contributions and assistance. Funding was provided by the Agriculture and Food Research Initiative, Biomass Research and Development Initiative, Competitive Grant no. 2010-05325 from the USDA National Institute of Food and Agriculture. NR 94 TC 0 Z9 0 U1 11 U2 17 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 727 EP 741 DI 10.2136/sssaj2015.11.0413 PG 15 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900019 ER PT J AU Han, YZ Zhang, JW Mattson, KG Zhang, WD Weber, TA AF Han, Youzhi Zhang, Jianwei Mattson, Kim G. Zhang, Weidong Weber, Thomas A. TI Sample Sizes to Control Error Estimates in Determining Soil Bulk Density in California Forest Soils SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID COMPACTION; CARBON; PLANTATIONS; MATTER; POOLS AB Characterizing forest soil properties with high variability is challenging, sometimes requiring large numbers of soil samples. Soil bulk density is a standard variable needed along with element concentrations to calculate nutrient pools. This study aimed to determine the optimal sample size, the number of observation (n), for predicting the soil bulk density with a precision of +/- 10% at a 95% confidence level among different soil types. We determined soil bulk density samples at three depths at 186 points distributed over three different 1-ha forest sites. We calculated n needed for estimating means of bulk density using a traditional method. This estimate was compared to a bootstrapping method n where the variance was estimated by re-sampling our original sample over 500 times. The results showed that patterns of soil bulk density varied by sites. Bootstrapping indicated 3 to 17 samples were needed to estimate mean soil bulk density at +/- 10% at a 95% confidence level at the three sites and three depths. Sample sizes determined by the bootstrap method were larger than the numbers estimated by the traditional method. Bootstrapping is considered theoretically to be more robust, especially at a site with more variability or for site measures that are not normally distributed. C1 [Han, Youzhi] Shanxi Agr Univ, Coll Forestry, Taigu 030801, Shanxi, Peoples R China. [Zhang, Jianwei] US Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. [Mattson, Kim G.] Ecosyst Northwest, 189 Shasta Ave, Mt Shasta, CA 96067 USA. [Zhang, Weidong] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Liaoning, Peoples R China. [Weber, Thomas A.] US Forest Serv, No Res Stn, 11 Campus Blvd,Ste 200, Newtown Sq, PA 19073 USA. RP Zhang, JW (reprint author), US Forest Serv, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. EM jianweizhang@fs.fed.us FU Shanxi Agricultural University through the Shanxi Scholarship Council of China; Institute of Applied Ecology, Chinese Academy of Sciences; Sierra Cascade Intensive Forest Management Research Cooperative FX Youzhi Han and Weidong Zhang were visiting scholars when this study was performed at the Pacific Southwest Research Station in Redding, CA. Youzhi Han was supported by Shanxi Agricultural University through the Shanxi Scholarship Council of China in 2015. Weidong Zhang was supported by the Institute of Applied Ecology, Chinese Academy of Sciences, in 2014. Thomas A. Weber is a former forest technician. Partial financial support came from the Sierra Cascade Intensive Forest Management Research Cooperative. We thank Crane Mills, Sierra Pacific Industries, and W.B. Beaty & Associates for managing these plantations on their land. The comments from Dr. John Brown, Dr. Linda Heath, and four anonymous reviewers for improving the manuscript are greatly appreciated. The use of trade names and commercial products in this article is solely for the purpose of providing specific information and does not constitute endorsement by the US Forest Service. NR 26 TC 0 Z9 0 U1 3 U2 3 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 756 EP 764 DI 10.2136/sssaj2015.12.0422 PG 9 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900021 ER PT J AU Veverica, TJ Kane, ES Marcarelli, AM Green, SA AF Veverica, Timothy J. Kane, Evan S. Marcarelli, Amy M. Green, Sarah A. TI Ionic Liquid Extraction Unveils Previously Occluded Humic-Bound Iron in Peat Soil Pore Water SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID DISSOLVED ORGANIC-MATTER; QUANTITATIVE ASSAY; CARBON MINERALIZATION; REDUCTION-OXIDATION; ELECTRON-ACCEPTORS; FERROZINE METHOD; PARALLEL FACTOR; FULVIC-ACID; FLUORESCENCE; 1,10-PHENANTHROLINE AB Globally, peatland ecosystems store tremendous amounts of C relative to their extent on the landscape, largely owing to saturated soils which limit decomposition. While there is still considerable uncertainty regarding CO2 production potential below the water table in peatland ecosystems, extracellular Fe reduction has been suggested as a dominant pathway for anaerobic metabolism. However, colorimetric methods commonly used to quantitate Fe and partition between redox species are known to be unreliable in the presence of complex humic substances, which are common in peatland pore water. We evaluated both the standard o-phenanthroline (o-P) Method and an ionic liquid extraction (ILE) Method followed by quantitation with inductively coupled plasma optical emission spectrometry (ICP-OES) to compare total Fe recovery and Fe2+/Fe3+ ratios in four distinct peatland ecosystems, ranging from rich fen to bog. While total Fe concentrations measured with ILE and o-P were correlated, the ILE method proved to be superior in both total Fe quantitation and in separately quantifying ferric (Fe3+) and ferrous (Fe2+) iron. In peat pore water, the o-P Method underestimated Fe3+ by as much as 100%. A multivariate approach utilizing fluorescence-and ultraviolet (UV)-visable (Vis) spectroscopy identified indices of dissolved organic matter (DOM) humification and redox status that correlated with poor performance of the o-P Method in peat pore water. Where these interferences are present, we suggest that site-specific empirical correction factors for quantitation of total Fe by o-P can be created from ILE of Fe, but recommend ILE for accurate appraisals of iron speciation and redox processes. C1 [Veverica, Timothy J.; Kane, Evan S.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Veverica, Timothy J.; Kane, Evan S.] USDA Forest Serv, No Res Stn, Houghton, MI 49931 USA. [Marcarelli, Amy M.] Michigan Technol Univ, Dept Biol Sci, Houghton, MI 49931 USA. [Green, Sarah A.] Michigan Technol Univ, Dept Chem, Houghton, MI 49931 USA. RP Kane, ES (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA.; Kane, ES (reprint author), USDA Forest Serv, No Res Stn, Houghton, MI 49931 USA. EM eskane@mtu.edu OI Marcarelli, Amy/0000-0002-4175-9211; Green, Sarah/0000-0002-8094-433X FU NSF [DEB-1146149, DEB LTREB 1354370]; Research Excellence Fund (Seed Grant) at Michigan Technological University; Ecosystem Science Center at Michigan Technological University FX This work was supported by funds from NSF DEB-1146149, NSF DEB LTREB 1354370, the Research Excellence Fund (Seed Grant) at Michigan Technological University and the Ecosystem Science Center at Michigan Technological University. In-kind support was received from the U.S. Forest Service, Northern Research Station. We thank Rasmus Bro, Rose Cory, and the staff of Eigenvector Research, Inc. for thoughtful discussion on chemometric methods. We thank Lynette Potvin, Jennifer Eikenberry, John Larson, Eryn Grupido, Sam Mosier, Kayla Griffith, Karl Meingast, and Brian Fisk for technical assistance. NR 64 TC 0 Z9 0 U1 11 U2 20 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 EI 1435-0661 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD MAY-JUN PY 2016 VL 80 IS 3 BP 771 EP 782 DI 10.2136/sssaj2015.10.0377 PG 12 WC Soil Science SC Agriculture GA DP9WP UT WOS:000378848900023 ER PT J AU Weaver, JT Malladi, S Bonney, PJ Patyk, KA Bergeron, JG Middleton, JL Alexander, CY Goldsmith, TJ Halvorson, DA AF Weaver, J. Todd Malladi, Sasidhar Bonney, Peter J. Patyk, Kelly A. Bergeron, Justin G. Middleton, Jamie L. Alexander, Catherine Y. Goldsmith, Timothy J. Halvorson, David A. TI A Simulation-Based Evaluation of Premovement Active Surveillance Protocol Options for the Managed Movement of Turkeys to Slaughter During an Outbreak of Highly Pathogenic Avian Influenza in the United States SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE highly pathogenic avian influenza; business continuity; risk assessment ID POULTRY FARMS; VIRUS; CHICKENS; EPIDEMIC; FLOCK; TIME; NETHERLANDS; INFECTION; IMPACT; EGGS AB Risk management decisions associated with live poultry movement during a highly pathogenic avian influenza (HPAI) outbreak should be carefully considered. Live turkey movements may pose a risk for disease spread. On the other hand, interruptions in scheduled movements can disrupt business continuity. The Secure Turkey Supply (STS) Plan was developed through an industry-government-academic collaboration to address business continuity concerns that might arise during a HPAI outbreak. STS stakeholders proposed outbreak response measure options that were evaluated through risk assessment. The developed approach relies on 1) diagnostic testing of two pooled samples of swabs taken from dead turkeys immediately before movement via the influenza A matrix gene real-time reverse transcriptase polymerase chain reaction (rRT-PCR) test; 2) enhanced biosecurity measures in combination with a premovement isolation period (PMIP), restricting movement onto the premises for a few days before movement to slaughter; and 3) incorporation of a distance factor from known infected flocks such that exposure via local area spread is unlikely. Daily exposure likelihood estimates from spatial kernels from past HPAI outbreaks were coupled with simulation models of disease spread and active surveillance to evaluate active surveillance protocol options that differ with respect to the number of swabs per pooled sample and the timing of the tests in relation to movement. Simulation model results indicate that active surveillance testing, in combination with strict biosecurity, substantially increased HPAI virus detection probability. When distance from a known infected flock was considered, the overall combined likelihood of moving an infected, undetected turkey flock to slaughter was predicted to be lower at 3 and 5 km. The analysis of different active surveillance protocol options is designed to incorporate flexibility into HPAI emergency response plans. C1 [Weaver, J. Todd; Patyk, Kelly A.] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv Sci Technol & Anal Serv, Ctr Epidemiol & Anim Hlth,Nat Resource Res Ctr, Bldg B,2150 Ctr Ave, Ft Collins, CO 80526 USA. [Malladi, Sasidhar; Bonney, Peter J.; Bergeron, Justin G.; Middleton, Jamie L.; Alexander, Catherine Y.; Goldsmith, Timothy J.] Univ Minnesota, Ctr Anim Hlth & Food Safety, Andrew Boss Lab 136, 1354 Eckles Ave, St Paul, MN 55108 USA. [Halvorson, David A.] Univ Minnesota, Coll Vet Med, 1971 Commonwealth Ave, St Paul, MN 55108 USA. RP Weaver, JT (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Vet Serv Sci Technol & Anal Serv, Ctr Epidemiol & Anim Hlth,Nat Resource Res Ctr, Bldg B,2150 Ctr Ave, Ft Collins, CO 80526 USA. EM todd.weaver@aphis.usda.gov NR 33 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 132 EP 145 PG 14 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900007 ER PT J AU Gardin, Y Palya, V Dorsey, KM El-Attrache, J Bonfante, F de Wit, S Kapczynski, D Kilany, WH Rauw, F Steensels, M Soejoedono, RD AF Gardin, Yannick Palya, Vilmos Dorsey, Kristi Moore El-Attrache, John Bonfante, Francesco de Wit, Sjaak Kapczynski, Darrell Kilany, Walid Hamdy Rauw, Fabienne Steensels, Mieke Soejoedono, Retno D. TI Experimental and Field Results Regarding Immunity Induced by a Recombinant Turkey Herpesvirus H5 Vector Vaccine Against H5N1 and Other H5 Highly Pathogenic Avian Influenza Virus Challenges SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE avian influenza; vaccination; rHVT-H5 vector vaccine; hatcheries; field trials ID PROTECTION; CHICKENS; EFFICACY; STRAINS AB Vaccination against H5N1 highly pathogenic avian influenza (AI) virus (HPAIV) is one of the possible complementary means available for affected countries to control AI when the disease has become, or with a high risk of becoming, endemic. Efficacy of the vaccination against AI relies essentially, but not exclusively, on the capacity of the vaccine to induce immunity against the targeted virus (which is prone to undergo antigenic variations), as well as its capacity to overcome interference with maternal immunity transmitted by immunized breeding hens to their progeny. This property of the vaccine is a prerequisite for its administration at the hatchery, which assures higher and more reliable vaccine coverage of the populations than vaccination at the farm. A recombinant vector vaccine (Vectormune (R) AI), based on turkey herpesvirus expressing the hemagglutinin gene of an H5N1 HPAIV as an insert, has been used in several experiments conducted in different research laboratories, as well as in controlled field trials. The results have demonstrated a high degree of homologous and cross protection against different genetic clades of the H5N1 HPAIV. Furthermore, vaccine-induced immunity was not impaired by the presence of passive immunity, but on the contrary, cumulated with it for improved early protection. The demonstrated levels of protection against the different challenge viruses exhibited variations in terms of postchallenge mortality, as well as challenge virus shedding. The data presented here highlight the advantages of this vaccine as a useful and reliable tool to complement biosecurity and sanitary policies for better controlling the disease due to HPAIV of H5 subtypes, when the vaccination is applied as a control measure. C1 [Gardin, Yannick] Ceva Anim Hlth, F-33501 Libourne, France. [Palya, Vilmos] Ceva Anim Hlth, H-1107 Budapest, Hungary. [Dorsey, Kristi Moore; El-Attrache, John] Ceva Anim Hlth, Lenexa, KS 66215 USA. [Bonfante, Francesco] Ist Zooprofilatt Sperimentale Venezie, I-35020 Padua, Italy. [de Wit, Sjaak] Gezondheidsdienst Dieren Anim Hlth, NL-7400 AA Deventer, Netherlands. [Kapczynski, Darrell] USDA, SE Poultry Res Lab, Athens, GA 30605 USA. [Kilany, Walid Hamdy] Agr Res Ctr, Minist Agr, Anim Hlth Res Inst, Natl Reference Lab Vet Qual Control Poultry Prod, PO 12618 Box 264, Giza, Egypt. [Rauw, Fabienne; Steensels, Mieke] Vet & Agrochem Res Ctr, Avian Virol & Immunol Unit, B-1180 Brussels, Belgium. [Soejoedono, Retno D.] Bogor Agr Univ, Fac Vet Med, Bogor 16680, Indonesia. RP Gardin, Y (reprint author), Ceva Sante Anim, 10 Ave Ballastiere, F-33500 Libourne, France. EM yannick.gardin@ceva.com OI Kilany, Walid/0000-0002-4242-507X NR 19 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 232 EP 237 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900018 PM 27309060 ER PT J AU Costa-Hurtado, M Afonso, CL Miller, PJ Shepherd, E DeJesus, E Smith, D Pantin-JackwoodA, MJ AF Costa-Hurtado, Mar Afonso, Claudio L. Miller, Patti J. Shepherd, Eric DeJesus, Eric Smith, Diane Pantin-JackwoodA, Mary J. TI Effect of Infection with a Mesogenic Strain of Newcastle Disease Virus on Infection with Highly Pathogenic Avian Influenza Virus in Chickens SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE highly pathogenic avian influenza; Newcastle disease virus; virus interference; coinfection ID VACCINE; COINFECTION; PCR AB Little is known on the interactions between avian influenza virus (AIV) and Newcastle disease virus (NDV) when coinfecting the same poultry host. In a previous study we found that infection of chickens with a mesogenic strain of NDV (mNDV) can reduce highly pathogenic AIV (HPAIV) replication, clinical disease, and mortality. This interaction depended on the titer of the viruses used and the timing of the infections. To further explore the effect of mNDV infectious dose in protecting chickens against HPAIV infection, 2-wk-old birds were inoculated with different doses of mNDV (10(4), 10(6), or 10(7) 50% embryo infective dose [EID50]) 3 days before inoculation with a HPAIV (10(5) or 10(6) EID50). Although birds coinfected with the higher mNDV doses (10(6) or 10(7)) survived for longer than birds inoculated only with HPAIV (10(5)), we did not observe the same protection with the lower dose of mNDV (10(4)) or when given the higher dose of HPAIV (10(6)), indicating that the relation between the titer of the two coinfecting viruses is determinant in the outcome. In a similar experiment, a higher number of 4-wk-old birds survived, and for longer, even when given higher HPAIV doses (10(6.3) and 10(7.3) EID50). In addition, we also examined the duration of protection provided by mNDV (10(7) EID50) on a HPAIV infection. Five-week-old chickens were inoculated with mNDV followed by inoculation with 10(6) EID50 of an HPAIV given at 2, 4, 6, or 9 days after the mNDV. HPAIV replication was affected and an increase in survival was found in all coinfected groups when compared to the HPAIV single-inoculated group, but the mortality in coinfected groups was high. In conclusion, previous inoculation with mNDV can affect HPAIV replication in chickens for at least 9 days, but this viral interference is titer dependent. C1 [Costa-Hurtado, Mar; Afonso, Claudio L.; Miller, Patti J.; Shepherd, Eric; DeJesus, Eric; Smith, Diane; Pantin-JackwoodA, Mary J.] ARS, Exot & Emerging Avian Viral Dis Unit, SE Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. RP Pantin-JackwoodA, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Unit, SE Poultry Res Lab, US Natl Poultry Res Ctr,USDA, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ars.usda.gov RI Costa-Hurtado, Mar/R-1496-2016 OI Costa-Hurtado, Mar/0000-0003-4194-0253 NR 22 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 269 EP 278 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900025 PM 27309067 ER PT J AU Dimitrov, KM Zarkov, IS Dinev, I Goujgoulova, GV Miller, PJ Suarez, DL AF Dimitrov, Kiril M. Zarkov, Ivan S. Dinev, Ivan Goujgoulova, Gabriela V. Miller, Patti J. Suarez, David L. TI Histopathologic Characterization and Shedding Dynamics of Guineafowl (Numida meleagris) Intravenously Infected with a H6N2 Low Pathogenicity Avian Influenza Virus SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE avian influenza; H6N2; guineafowl; LPAI; shedding; histopathology; rRT-PCR ID LIVE-BIRD MARKETS; COMPARATIVE PATHOLOGY; SOUTHERN CHINA; DOMESTIC DUCKS; A VIRUS; CHICKENS; ORIGIN; WATERFOWL; PATHOBIOLOGY; INOCULATION AB Guineafowl of different ages were inoculated intravenously with a H6N2 wild waterfowl-origin low pathogenicity avian influenza virus (LPAIV). No clinical disease was observed. The infected birds had atrophy of the spleen, thymus, and cloacal bursa when compared with the noninfected control groups. The central and peripheral lymphoid tissues presented either lymphoproliferative or degenerative lesions that increased in intensity from 14 to 21 days postinoculation (DPI). Lymphoid depletion was present in the bursa, thymic lobes, and spleen T-dependent zone. In contrast, lymphoid proliferation was observed in liver, pancreas, and spleen B-dependent zone. Bronchus associated lymphoid tissue hyperplasia was observed in the lungs of the birds at 14 and 21 DPI. The virus was detected by virus isolation and reverse transcription PCR from both oropharyngeal and cloacal swabs with higher isolation rates from the latter. Most birds from the LPAIV inoculated groups shed virus up to 7 DPI. The virus was infrequently isolated from lung, kidney, liver, bursa, or spleen of infected birds until 14 DPI and from two samples (kidney and spleen, 1-yr-old birds) at 21 DPI. These data indicate that the wild bird-origin LPAIV used in this study caused pantropic infection in guineafowl when inoculated intravenously. C1 [Dimitrov, Kiril M.; Goujgoulova, Gabriela V.] Natl Diagnost & Res Vet Med Inst, 15 P Slaveikov Blvd, Sofia 1606, Bulgaria. [Dimitrov, Kiril M.; Miller, Patti J.; Suarez, David L.] ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. [Zarkov, Ivan S.] Trakia Univ, Fac Vet Med, Dept Microbiol Infect & Parasite Dis, Students Campus, Stara Zagora 6000, Bulgaria. [Dinev, Ivan] Trakia Univ, Fac Vet Med, Dept Gen & Clin Pathol, Students Campus, Stara Zagora 6000, Bulgaria. RP Dimitrov, KM (reprint author), Natl Diagnost & Res Vet Med Inst, 15 P Slaveikov Blvd, Sofia 1606, Bulgaria.; Dimitrov, KM (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM dimitrov_kiril@yahoo.com NR 58 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 279 EP 285 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900026 PM 27309068 ER PT J AU Bailey, E Long, LP Zhao, N Hall, JS Baroch, JA Nolting, J Senter, L Cunningham, FL Pharr, GT Hanson, L Slemons, R DeLiberto, TJ Wanag, XF AF Bailey, Elizabeth Long, Li-Ping Zhao, Nan Hall, Jeffrey S. Baroch, John A. Nolting, Jacqueline Senter, Lucy Cunningham, Frederick L. Pharr, G. Todd Hanson, Larry Slemons, Richard DeLiberto, Thomas J. Wanag, Xiu-Feng TI Antigenic Characterization of H3 Subtypes of Avian Influenza A Viruses from North America SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE influenza A virus; avian influenza virus; migratory waterfowl; H3; low pathogenic avian influenza virus; antigenic cartography; migratory flyway ID UNITED-STATES; EXPERIMENTAL-INFECTION; HIGH-THROUGHPUT; SWINE; TRANSMISSION; EVOLUTION; ORIGIN; MORTALITY; ASIA; HEMAGGLUTININ AB Besides humans, H3 subtypes of influenza A viruses (IAVs) can infect various animal hosts, including avian, swine, equine, canine, and sea mammal species. These H3 viruses are both antigenically and genetically diverse. Here, we characterized the antigenic diversity of contemporary H3 avian IAVs recovered from migratory birds in North America. Hemagglutination inhibition (HI) assays were performed on 37 H3 isolates of avian IAVs recovered from 2007 to 2011 using generated reference chicken sera. These isolates were recovered from samples taken in the Atlantic, Mississippi, Central, and Pacific waterfowl migration flyways. Antisera to all the tested H3 isolates cross-reacted with each other and, to a lesser extent, with those to H3 canine and H3 equine IAVs. Antigenic cartography showed that the largest antigenic distance among the 37 avian IAVs is about four units, and each unit corresponds to a 2 log & thinsp;(2) difference in the HI titer. However, none of the tested H3 IAVs cross-reacted with ferret sera derived from contemporary swine and human IAVs. Our results showed that the H3 avian IAVs we tested lacked significant antigenic diversity, and these viruses were antigenically different from those circulating in swine and human populations. This suggests that H3 avian IAVs in North American waterfowl are antigenically relatively stable. C1 [Bailey, Elizabeth; Long, Li-Ping; Zhao, Nan; Pharr, G. Todd; Hanson, Larry; Wanag, Xiu-Feng] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Hall, Jeffrey S.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA. [Baroch, John A.; DeLiberto, Thomas J.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Nolting, Jacqueline; Slemons, Richard] Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA. [Senter, Lucy] Mississippi State Univ, Coll Vet Med, Dept Clin Sci, Mississippi State, MS 39762 USA. [Cunningham, Frederick L.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr,Mississippi Field Stn, Mississippi State, MS 39762 USA. RP Wanag, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM wan@cvm.msstate.edu FU NIAID NIH HHS [R01 AI116744] NR 54 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 346 EP 353 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900036 PM 27309078 ER PT J AU Ip, HS Dusek, RJ Bodenstein, B Torchetti, MK DeBruyn, P Mansfield, KG DeLiberto, T Sleeman, JM AF Ip, Hon S. Dusek, Robert J. Bodenstein, Barbara Torchetti, Mia Kim DeBruyn, Paul Mansfield, Kristin G. DeLiberto, Thomas Sleeman, Jonathan M. TI High Rates of Detection of Clade 2.3.4.4 Highly Pathogenic Avian Influenza H5 Viruses in Wild Birds in the Pacific Northwest During the Winter of 2014-15 SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE avian influenza; intercontinental transmission; wild; migratory birds; surveillance strategy; HPAIV; H5Nx ID A(H5N8) VIRUS; SOUTH-KOREA; POULTRY; DUCKS; CHINA AB In 2014, clade 2.3.4.4 H5N8 highly pathogenic avian influenza (HPAI) viruses spread across the Republic of Korea and ultimately were reported in China, Japan, Russia, and Europe. Mortality associated with a reassortant HPAI H5N2 virus was detected in poultry farms in western Canada at the end of November. The same strain (with identical genetic structure) was then detected in free-living wild birds that had died prior to December 8, 2014, of unrelated causes in Whatcom County, Washington, U. S. A., in an area contiguous with the index Canadian location. A gyrfalcon (Falco rusticolus) that had hunted and fed on an American wigeon (Anas americana) on December 6, 2014, in the same area, and died 2 days later, tested positive for the Eurasian-origin HPAI H5N8. Subsequently, an active surveillance program using hunter-harvested waterfowl in Washington and Oregon detected 10 HPAI H5 viruses, of three different subtypes (four H5N2, three H5N8, and three H5N1) with four segments in common (HA, PB2, NP, and MA). In addition, a mortality-based passive surveillance program detected 18 HPAI (14 H5N2 and four H5N8) cases from Idaho, Kansas, Oregon, Minnesota, Montana, Washington, and Wisconsin. Comparatively, mortality-based passive surveillance appears to have detected these HPAI infections at a higher rate than active surveillance during the period following initial introduction into the United States. C1 [Ip, Hon S.; Dusek, Robert J.; Bodenstein, Barbara; Sleeman, Jonathan M.] US Geol Survey, Natl Wildlife Hlth Ctr, 6006 Schroeder Rd, Madison, WI 53711 USA. [Torchetti, Mia Kim] USDA, Natl Vet Serv Labs, 1920 Dayton Ave, Ames, IA 50010 USA. [DeBruyn, Paul; Mansfield, Kristin G.] Washington Dept Fish & Wildlife, 2315 N Discovery Pl, Olympia, WA 99216 USA. [DeLiberto, Thomas] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Ip, HS (reprint author), US Geol Survey, Natl Wildlife Hlth Ctr, 6006 Schroeder Rd, Madison, WI 53711 USA. EM hip@usgs.gov NR 20 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 354 EP 358 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900037 PM 27309079 ER PT J AU Muzyka, D Pantin-Jackwood, M Spackman, E Smith, D Rula, O Muzyka, N Stegniy, B AF Muzyka, Denys Pantin-Jackwood, Mary Spackman, Erica Smith, Diane Rula, Oleksandr Muzyka, Nataliia Stegniy, Borys TI Isolation and Genetic Characterization of Avian Influenza Viruses Isolated from Wild Birds in the Azov-Black Sea Region of Ukraine (2001-2012) SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Avian Influenza CY APR 12-15, 2015 CL Univ Georgia, Ctr Continuing Educ, Athens, GA SP Merial, Merck Anim Hlth, CEVA, Amer Assoc Avian Pathologists, Ctr Dis Control & Prevent, Boehringer Ingelheim Anim Hlth, US Dept Agr, Anim & Plant Hlth Inspect Serv Wildlife Serv, US Dept Agr, Agr Res Serv, US Dept Interior, US Geol Surv, Zoetis Anim Hlth, OIE FAO Anim Influenza Network, United Nations Food & Agr Org, World Org Anim Hlth HO Univ Georgia, Ctr Continuing Educ DE avian influenza; wild birds; surveillance; Azov-Black Sea region of Ukraine ID A-VIRUS; ATLANTIC FLYWAY; NORTH-AMERICA; DUCKS; SURVEILLANCE; ANTIBODIES; PREVALENCE; PARAMYXOVIRUSES; SHOREBIRDS; INFECTION AB Wild bird surveillance for avian influenza virus (AIV) was conducted from 2001 to 2012 in the Azov - Black Sea region of the Ukraine, considered part of the transcontinental wild bird migration routes from northern Asia and Europe to the Mediterranean, Africa, and southwest Asia. A total of 6281 samples were collected from wild birds representing 27 families and eight orders for virus isolation. From these samples, 69 AIVs belonging to 15 of the 16 known hemagglutinin (HA) subtypes and seven of nine known neuraminidase (NA) subtypes were isolated. No H14, N5, or N9 subtypes were identified. In total, nine H6, eight H1, nine H5, seven H7, six H11, six H4, five H3, five H10, four H8, three H2, three H9, one H12, one H13, one H15, and one H16 HA subtypes were isolated. As for the NA subtypes, twelve N2, nine N6, eight N8, seven N7, six N3, four N4, and one undetermined were isolated. There were 27 HA and NA antigen combinations. All isolates were low pathogenic AIV except for eight highly pathogenic (HP) AIVs that were isolated during the H5N1 HPAI outbreaks of 2006-08. Sequencing and phylogenetic analysis of the HA genes revealed epidemiological connections between the Azov-Black Sea regions and Europe, Russia, Mongolia, and Southeast Asia. H1, H2, H3, H7, H8, H6, H9, and H13 AIV subtypes were closely related to European, Russian, Mongolian, and Georgian AIV isolates. H10, H11, and H12 AIV subtypes were epidemiologically linked to viruses from Europe and Southeast Asia. Serology conducted on serum and egg yolk samples also demonstrated previous exposure of many wild bird species to different AIVs. Our results demonstrate the great genetic diversity of AIVs in wild birds in the Azov-Black Sea region as well as the importance of this region for monitoring and studying the ecology of influenza viruses. This information furthers our understanding of the ecology of avian influenza viruses in wild bird species. C1 [Muzyka, Denys; Rula, Oleksandr; Muzyka, Nataliia; Stegniy, Borys] Inst Expt & Clin Vet Med, Ctr Nat Sci, UA-61023 Kharkov, Ukraine. [Pantin-Jackwood, Mary; Spackman, Erica; Smith, Diane] ARS, USDA, SE Poultry Res Lab, Exot & Emerging Avian Viral Dis Unit, Watkinsville, GA 30677 USA. RP Muzyka, D (reprint author), Inst Expt & Clin Vet Med, Ctr Nat Sci, UA-61023 Kharkov, Ukraine. EM dmuzyka77@gmail.com NR 68 TC 0 Z9 0 U1 3 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD MAY PY 2016 VL 60 IS 1 SU S BP 365 EP 377 PG 13 WC Veterinary Sciences SC Veterinary Sciences GA DO9EA UT WOS:000378085900039 PM 27309081 ER PT J AU Grindal, T Wilde, P Schwartz, G Klerman, J Bartlett, S Berman, D AF Grindal, Todd Wilde, Parke Schwartz, Gabe Klerman, Jacob Bartlett, Susan Berman, Danielle TI Does food retail access moderate the impact of fruit and vegetable incentives for SNAP participants? Evidence from western Massachusetts SO FOOD POLICY LA English DT Article DE Supplemental Nutrition Assistance Program; Incentives; Fruits and vegetables; Spatial analysis; Healthy Incentives Pilot; Food retail ID STAMP PROGRAM; UNITED-STATES; STORE ACCESS; AVAILABILITY; CONSUMPTION; PRICES; DIET; NEIGHBORHOODS; SUPERMARKETS; PERCEPTIONS AB Objectives: This study investigates whether the response of Supplemental Nutrition Assistance Program (SNAP) participants to a 30% incentive on fruit and vegetable spending varies with their access to food retailers. Methods: The analysis exploits the random assignment of SNAP households in Hampden County, MA, to an intervention group that earned the incentive. Regression models for the impact of the incentive are augmented with measures of food retail access and interactions of random assignment status with food retail access. The main outcome use of the SNAP benefit is based on Electronic Benefit Transfer (EBT) card transaction records. Results: Although households that lived within a mile of a participating supermarket spent approximately $2.13 or 19% more per month on targeted fruits and vegetables at participating supermarkets than households that did not live within a mile of a participating supermarket, we found no evidence that the impact of the incentive on SNAP fruit and vegetable spending varies with distance to retailers. Conclusions: These findings imply that incentives to purchase fruits and vegetables were equally efficacious for SNAP households with high and low access to food retailers. (C) 2016 Published by Elsevier Ltd. C1 [Grindal, Todd; Schwartz, Gabe; Klerman, Jacob; Bartlett, Susan] ABT Associates Inc, Cambridge, MA 02138 USA. [Wilde, Parke] Tufts Univ, Friedman Sch Nutr Sci & Policy, Medford, MA USA. [Berman, Danielle] USDA, Food & Nutr Serv, Washington, DC USA. RP Grindal, T (reprint author), ABT Associates Inc, Cambridge, MA 02138 USA. OI Schwartz, Gabriel/0000-0001-8299-1407 NR 36 TC 2 Z9 2 U1 6 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 EI 1873-5657 J9 FOOD POLICY JI Food Policy PD MAY PY 2016 VL 61 BP 59 EP 69 DI 10.1016/j.foodpol.2016.02.002 PG 11 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA DP4HD UT WOS:000378456100005 ER PT J AU Zellner, WL AF Zellner, Wendy L. TI Response to Comments by Provance-Bowley on Continuing Assessment of the 5-Day Sodium Carbonate-Ammonium Nitrate Extraction Assay as an Indicator Test for Silicon Fertilizers SO JOURNAL OF AOAC INTERNATIONAL LA English DT Letter C1 [Zellner, Wendy L.] ARS, USDA, Greenhouse Prod Res Grp, 2801 W Bancroft St,Mail Stop 604, Toledo, OH 43606 USA. RP Zellner, WL (reprint author), ARS, USDA, Greenhouse Prod Res Grp, 2801 W Bancroft St,Mail Stop 604, Toledo, OH 43606 USA. EM wendy.zellner@ars.usda.gov NR 7 TC 0 Z9 0 U1 2 U2 2 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD MAY-JUN PY 2016 VL 99 IS 3 BP 33A EP 34A DI 10.5740/jaoacint.16-0028 PG 2 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA DP0OI UT WOS:000378188700004 ER PT J AU Albright, VC Hellmich, RL Coats, JR AF Albright, Vurtice C., III Hellmich, Richard L. Coats, Joel R. TI Development of a Model System Approach for Generating Fragments of the Cry1Ab Protein SO JOURNAL OF AOAC INTERNATIONAL LA English DT Article ID KURSTAKI DELTA-ENDOTOXIN; IN-VITRO DIGESTIBILITY; BACILLUS-THURINGIENSIS; FOOD ALLERGENS; DEGRADATION; DIGESTION; SOIL; BIOTECHNOLOGY; DIVERSITY; SUNLIGHT AB The use of transgenic crops expressing one or more crystal (Cry) proteins for insect management has grown dramatically since their introduction nearly 2 decades ago. However, many questions surrounding the environmental fate of these proteins still persist. One area of particular interest is the possible detection of Cry protein fragments by the antibodies used in ELISA kits. A model system approach is used to generate environmentally relevant fragments by simulating conditions and digestive enzymes that are known to exist in environments in which Cry proteins may be present. Seven different types of model systems were screened for their ability to generate fragments of the Cry1Ab protein; five of these model systems reliably generated Cry1Ab fragments. These fragments were analyzed in a subsequent study to determine whether the fragments were still detectable by ELISA and whether they retained any bioactivity. C1 [Albright, Vurtice C., III; Coats, Joel R.] Iowa State Univ, Dept Entomol, Pesticide Toxicol Lab, 115 Insectary, Ames, IA 50011 USA. [Hellmich, Richard L.] ARS, USDA, Corn Insects & Crop Genet Res Unit, Genet Lab 110, Ames, IA 50011 USA. RP Coats, JR (reprint author), Iowa State Univ, Dept Entomol, Pesticide Toxicol Lab, 115 Insectary, Ames, IA 50011 USA. EM jcoats@iastate.edu NR 34 TC 0 Z9 0 U1 2 U2 2 PU AOAC INT PI GAITHERSBURG PA 481 N FREDRICK AVE, STE 500, GAITHERSBURG, MD 20877-2504 USA SN 1060-3271 EI 1944-7922 J9 J AOAC INT JI J. AOAC Int. PD MAY-JUN PY 2016 VL 99 IS 3 BP 792 EP 805 DI 10.5740/jaoacint.15-0229 PG 14 WC Chemistry, Analytical; Food Science & Technology SC Chemistry; Food Science & Technology GA DP0OI UT WOS:000378188700031 ER PT J AU Volk, GM Henk, AD AF Volk, Gayle M. Henk, Adam D. TI Historic American Apple Cultivars: Identification and Availability SO JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE LA English DT Article ID PLANT GERMPLASM SYSTEM; UNITED-STATES; DIVERSITY; CONSERVATION; COLLECTIONS; PREFERENCES; GENEBANKS; VARIETIES; TREES; FRUIT AB Apples (Malus Mill.) have been economically and socially important throughout the centuries in North America. Apple cultivar (Malus x domestica Borkh.) collections that include historic cultivars are valued for their unique diversity, historical significance, and also as a resource to identify unknown trees; however, not all of the historically significant apple cultivars are currently included in these collections. We used historic books, publications, and nursery catalogs to develop an inventory of apple cultivars that were propagated and grown in the United States before 1908. We collected synonym, introduction date, and original source country information for 891 historic apple cultivars. Most of the historic American cultivars originated as seedlings first planted in the United States. Some cultivars were brought to the United States from the United Kingdom, France, Russia, Germany, and other European countries. We classified historic apple cultivars based on their availability over time and popularity in nursery catalogs. Ninety percent of the most popular historic apple cultivars in the United States were available in 2015 in the U.S. and U.K. national collections and within several commercial and private collections. This work identified high priority historic cultivars that are not currently protected within genebanks that could be added to genebank collections in the future. C1 [Volk, Gayle M.; Henk, Adam D.] ARS, USDA, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. RP Volk, GM (reprint author), ARS, USDA, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM gayle.volk@ars.usda.gov NR 55 TC 1 Z9 1 U1 1 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0003-1062 EI 2327-9788 J9 J AM SOC HORTIC SCI JI J. Am. Soc. Hortic. Sci. PD MAY PY 2016 VL 141 IS 3 BP 292 EP + PG 34 WC Horticulture SC Agriculture GA DO9XL UT WOS:000378141200010 ER PT J AU Melendez-Ackerman, EJ Nytch, CJ Santiago-Acevedo, LE Verdejo-Ortiz, JC Santiago-Bartolomei, R Ramos-Santiago, LE Munoz-Erickson, TA AF Melendez-Ackerman, Elvia J. Nytch, Christopher J. Santiago-Acevedo, Luis E. Verdejo-Ortiz, Julio C. Santiago-Bartolomei, Raul Ramos-Santiago, Luis E. Munoz-Erickson, Tischa A. TI Synthesis of Household Yard Area Dynamics in the City of San Juan Using Multi-Scalar Social-Ecological Perspectives SO SUSTAINABILITY LA English DT Article DE household yards; social ecological systems; green infrastructure (GI); sustainability; shrinking cities; ecosystem services ID RESIDENTIAL PESTICIDE USE; ECOSYSTEM SERVICES; SHRINKING CITIES; GREEN INFRASTRUCTURE; URBAN; BIODIVERSITY; GARDENS; MANAGEMENT; PATTERNS; URBANIZATION AB Urban sustainability discourse promotes the increased use of green infrastructure (GI) because of its contribution of important ecosystem services to city dwellers. Under this vision, all urban green spaces, including those at the household scale, are valued for their potential contributions to a city's social-ecological functioning and associated benefits for human well-being. Understanding how urban residential green spaces have evolved can help improve sustainable urban planning and design, but it requires examining urban processes occurring at multiple scales. The interaction between social structures and ecological structures within the subtropical city of San Juan, the capital and the largest city of Puerto Rico, has been an important focus of study of the San Juan ULTRA (Urban Long-Term Research Area) network, advancing understanding of the city's vulnerabilities and potential adaptive capacity. Here we provide a synthesis of several social-ecological processes driving residential yard dynamics in the city of San Juan, Puerto Rico, through the evaluation of empirical findings related to yard management decisions, yard area, and yard services. We emphasize the role of factors occurring at the household scale. Results are discussed within the context of shrinking cities using an integrated, multi-scalar, social-ecological systems framework, and consider the implications of household green infrastructure for advancing urban sustainability theory. C1 [Melendez-Ackerman, Elvia J.; Nytch, Christopher J.] Univ Puerto Rico, Dept Environm Sci, Rio Piedras Campus POB 70377, San Juan, PR 00931 USA. [Santiago-Acevedo, Luis E.; Verdejo-Ortiz, Julio C.] Univ Puerto Rico, Grad Sch Planning, Rio Piedras Campus,POB 23354, San Juan, PR 00931 USA. [Santiago-Bartolomei, Raul] Univ So Calif, Sol Price Sch Publ Policy, Lewis Hall 312, Los Angeles, CA 90089 USA. [Ramos-Santiago, Luis E.] Florida State Univ, Dept Urban & Reg Planning, 330 Bellamy Bldg,113 Collegiate Loop,POB 3062280, Tallahassee, FL 32306 USA. [Munoz-Erickson, Tischa A.] US Forest Serv, Int Inst Trop Forestry, 1201 Calle Ceiba, Rio Piedras, PR 00926 USA. RP Melendez-Ackerman, EJ (reprint author), Univ Puerto Rico, Dept Environm Sci, Rio Piedras Campus POB 70377, San Juan, PR 00931 USA. EM elmelend@gmail.com; chris.nytch@ites.upr.edu; santiago.luis47@gmail.com; juliocv@gmail.com; raulsant@usc.edu; estudiopd@gmail.com; tamunozerickson@fs.fed.us FU NSF San Juan ULTRA-Ex [DEB-0948507]; NSF-IGERT [HRD 00801577]; program Agents of Change, and CREST-CATEC [HRD-0734826]; Center for Applied Tropical Ecology and Conservation FX This work has been supported by NSF San Juan ULTRA-Ex (DEB-0948507), NSF-IGERT (HRD 00801577) and its program Agents of Change, and CREST-CATEC (HRD-0734826) and The Center for Applied Tropical Ecology and Conservation. We would like to thank Sofia Olivero, Angelica Erazo, Elizabeth Diaz, Khrisia Torres, Pedro Delgado, Alejandra Bonilla, Stella Gonzalez, Karla Torres, Jessenia Fontanez, Aramis Garay, Carla Lopez-Lloreda, Cristina Vila and Sofyaneli Colon and many graduate and undergraduate students for their help conducting residential surveys, and the residents of the RPWS for their willingness to participate in this study. NR 98 TC 1 Z9 1 U1 11 U2 11 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD MAY PY 2016 VL 8 IS 5 AR 481 DI 10.3390/su8050481 PG 21 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DO7SR UT WOS:000377983800075 ER PT J AU Kojima, Y Heitman, JL Flerchinger, GN Ren, TS Horton, R AF Kojima, Yuki Heitman, Joshua L. Flerchinger, Gerald N. Ren, Tusheng Horton, Robert TI Sensible Heat Balance Estimates of Transient Soil Ice Contents SO VADOSE ZONE JOURNAL LA English DT Article ID TIME-DOMAIN REFLECTOMETRY; UNFROZEN WATER-CONTENT; THERMAL-PROPERTIES; FROZEN SOIL; TEMPERATURE-GRADIENTS; THAWING PROCESSES; UNSATURATED SOIL; PULSE-METHOD; IN-SITU; EVAPORATION AB Soil ice content is an important component for winter soil hydrology. The sensible heat balance (SHB) method using measurements from heat pulse probes (HPPs) is a possible way to determine transient soil ice content. In a previous study, in situ soil ice content estimates with the SHB method were inaccurate, due to thermal conductivity errors and the use of relatively long time steps for calculations. The objective of this study is to reexamine the SHB method for soil ice content determination. A soil freezing and thawing laboratory experiment was performed with soil columns and heat exchangers. Transient soil ice contents in the soil columns during soil freezing and thawing were determined with the SHB method. The SHB method was able to determine dynamic changes in soil ice contents during initial freezing and final thawing for soil temperatures between -5 and 0 degrees C when latent heat values associated with ice formation or with thawing were relatively large. During an extended freezing period, when soil temperatures were below -5 degrees C, the small associated latent heat fluxes were below the sensitivity of the SHB method, and the SHB method did not provide accurate estimates of ice contents with time. However, the soil ice contents during the extended freezing period could be estimated well from changes in volumetric heat capacity (C) determined with HPP. Thus, combining the SHB method for initial freezing and final thawing, with a change in C method for extended freezing periods, allowed determination of dynamic soil ice contents for the entire range of freezing and thawing soil temperatures investigated. HPPs were able to measure soil ice contents. C1 [Kojima, Yuki] Gifu Univ, Dept Civil Engn, Gifu, Japan. [Heitman, Joshua L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Ren, Tusheng] China Agr Univ, Dept Soil & Water Sci, Beijing 100094, Peoples R China. [Horton, Robert] Iowa State Univ, Dept Agron, Ames, IA USA. RP Kojima, Y (reprint author), Gifu Univ, Dept Civil Engn, Gifu, Japan. EM kojima@gifu-u.ac.jp FU National Science Foundation [1215864]; Hatch Act, State of Iowa fund [3188]; Hatch Act, State of North Carolina fund [3188] FX This work was supported by the National Science Foundation under Grant 1215864, and by Multi-State Project 3188, Hatch Act, State of Iowa, and State of North Carolina funds. The authors thank Matthew A. Coyle for his contributions to this study. NR 48 TC 1 Z9 1 U1 9 U2 12 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD MAY PY 2016 VL 15 IS 5 DI 10.2136/vzj2015.10.0134 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DP2PW UT WOS:000378332400004 ER PT J AU Rada, N AF Rada, Nicholas TI India's post-green-revolution agricultural performance: what is driving growth? SO AGRICULTURAL ECONOMICS LA English DT Article DE Agriculture; Area expansion; Crop yield; India; Irrigation; Product diversification; Total factor productivity (TFP) ID PAKISTAN AB Following a period of poor performance in the 1990s, India's agricultural growth rate has reaccelerated in the 2000s. Some believe the reacceleration has been a product of intensified investment, which in turn has spurred yield growth. Others suggest it is because India's newly wealthy citizens have demanded greater product diversification. To examine these hypotheses, we use growth accounting techniques in conjunction with more complete agricultural production data than in past studies to construct state, regional, and national output, input, and total factor productivity quantity indexes, which can be decomposed into their underlying sources. Sectoral performance evaluation suggests that, since 1980, output growth has diffused away from the northern " grain belt" and toward high-value agriculture in traditionally less-productive regions. Productivity growth, rather than resource use, has accounted for these geographical and intensity shifts. The growth burst has not, as the literature has primarily argued, been uniquely explainable by yield growth or product diversification but by a variety of factors, including area expansion. For example, the contributions of irrigation technologies permitting double-cropping have until now been largely ignored. C1 [Rada, Nicholas] Econ Res Serv, USDA, Washington, DC 20024 USA. RP Rada, N (reprint author), Econ Res Serv, USDA, Washington, DC 20024 USA. EM nrada@ers.usda.gov NR 32 TC 0 Z9 0 U1 12 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0169-5150 EI 1574-0862 J9 AGR ECON-BLACKWELL JI Agric. Econ. PD MAY PY 2016 VL 47 IS 3 BP 341 EP 350 DI 10.1111/agec.12234 PG 10 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA DO5CT UT WOS:000377801900008 ER PT J AU Bobryk, CW Myers, DB Kitchen, NR Shanahan, JF Sudduth, KA Drummond, ST Gunzenhauser, B Raboteaux, NNG AF Bobryk, Christopher W. Myers, D. Brenton Kitchen, Newell R. Shanahan, John F. Sudduth, Kenneth A. Drummond, Scott T. Gunzenhauser, Bob Raboteaux, Nadilia N. Gomez TI Validating a Digital Soil Map with Corn Yield Data for Precision Agriculture Decision Support SO AGRONOMY JOURNAL LA English DT Article ID MANAGEMENT ZONE DELINEATION; ELECTRICAL-CONDUCTIVITY; GRAIN-YIELD; FUZZY-LOGIC; CLASSIFICATION TREES; NITROGEN MANAGEMENT; EXPERT KNOWLEDGE; DROUGHT; DISAGGREGATION; INFORMATION AB Capturing the variability in soil-landscape properties is a challenge for grain producers attempting to integrate spatial information into the decision process of precision agriculture (PA). Digital soil maps (DSMs) use traditional soil survey information and can be the basis for PA subfield delineation (e.g., management zones). However, public soil survey maps provide only general descriptions of soil-landscape features. Therefore, improved DSMs are needed that use high-resolution data that more precisely model soil-landscape characteristics. Additionally, reliable methods are needed to validate DSM products for PA. The objective of this study was to validate with corn (Zea mays L.) yield data the performance of a new DSM product, termed Environmental Response Unit (ERU), compared with the USDA Soil Survey Geographic (SSURGO) soil map. The ERU was developed by integrating SSURGO information with high-resolution elevation data. For validation, corn yield maps were collected and corrected for common data collection errors from 409 fields across Indiana, Iowa, Minnesota, and Nebraska in 2010 to 2012. Reductions in the area-weighted variance (R-v) of corn yield for ERU and SSURGO were calculated relative to the whole-field variance. The average R-v across all site-years for SSURGO and ERU was 16 and 25%, respectively, which equated to a 57% higher median yield variance reduction with ERU over SSURGO. This variance reduction technique showed the potential of ERU as an improved model better representing soil-landscape properties that impact corn yield. This research also has application potential for determining the success of a DSM for identifying management zones in PA. C1 [Bobryk, Christopher W.; Kitchen, Newell R.; Sudduth, Kenneth A.; Drummond, Scott T.] Univ Missouri, USDA ARS, Cropping Syst & Water Qual Res Unit, 269 Agr Engn Bldg, Columbia, MO 65211 USA. [Myers, D. Brenton; Shanahan, John F.; Gunzenhauser, Bob; Raboteaux, Nadilia N. Gomez] DuPont Pioneer, 7300 NW 62nd Ave,POB 1004, Johnston, IA 50131 USA. RP Bobryk, CW (reprint author), Univ Missouri, USDA ARS, Cropping Syst & Water Qual Res Unit, 269 Agr Engn Bldg, Columbia, MO 65211 USA. EM BobrykC@missouri.edu NR 71 TC 0 Z9 0 U1 17 U2 21 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2016 VL 108 IS 3 BP 957 EP 965 DI 10.2134/agronj2015.0381 PG 9 WC Agronomy SC Agriculture GA DO5DY UT WOS:000377805000003 ER PT J AU Siller, ARS Albrecht, KA Jokela, WE AF Siller, Arthur R. S. Albrecht, Kenneth A. Jokela, William E. TI Soil Erosion and Nutrient Runoff in Corn Silage Production with Kura Clover Living Mulch and Winter Rye SO AGRONOMY JOURNAL LA English DT Article ID AGGREGATE-SIZE INTERACTIONS; SOYBEAN-FORAGE ROTATION; COVER CROP MANAGEMENT; ORGANIC-MATTER; RAINFALL SIMULATOR; SMOOTH BROMEGRASS; RESIDUE REMOVAL; WATER-CONTENT; PLOT-SCALE; NO-TILL AB Corn (Zea mays L.) silage is a productive and popular forage crop that can exacerbate soil loss, surface water runoff, and nonpoint source nutrient pollution from agricultural fields. The objective of this research was to compare soil loss, nutrient runoff, and soil physical properties in conventional monocrop corn silage production to production in Kura clover (Trifolium ambiguum M. Bieb.) living mulch or with a winter rye (Secale cereale L.) cover crop. Five runoff events from simulated rainfall were conducted over a 1-yr period on loess soils with 8 to 15% slope. Based on weighted averages across all simulations, Kura clover living mulch reduced runoff by 50%, soil loss by 77%, and P and N losses by 80% relative to monocrop corn. Rye planted aft er corn silage harvest reduced runoff by 67%, soil loss by 81%, P loss by 94%, and N loss by 83%. When rye was planted following corn silage in Kura clover living mulch, runoff was reduced by 68%, soil loss by 77%, P loss by 94%, and N loss by 84% relative to monocrop corn. Greater groundcover in the Kura clover and rye treatments was the primary factor in reducing soil and nutrient losses, but soil aggregate stability and soil disturbance from rye planting were also associated with improved infiltration and reduced runnoff. When grown in Kura clover living mulch, both corn and rye had lower forage yields, a trade-off with the environmental benefits; but that economic loss would be partially off set by lower fertilizer requirements. C1 [Siller, Arthur R. S.; Albrecht, Kenneth A.] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Jokela, William E.] USDA ARS, US Dairy Forage Res Ctr, 2615 Yellowstone Dr, Marshfield, WI 54449 USA. RP Albrecht, KA (reprint author), Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. EM kaalbrec@wisc.edu NR 71 TC 1 Z9 1 U1 16 U2 23 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2016 VL 108 IS 3 BP 989 EP 999 DI 10.2134/agronj2015.0488 PG 11 WC Agronomy SC Agriculture GA DO5DY UT WOS:000377805000006 ER PT J AU Schlegel, AJ Assefa, Y Dumler, TJ Haag, LA Stone, LR Halvorson, AD Thompson, CR AF Schlegel, Alan J. Assefa, Yared Dumler, Troy J. Haag, Lucas A. Stone, Loyd R. Halvorson, Ardell D. Thompson, Curtis R. TI Limited Irrigation of Corn-Based No-Till Crop Rotations in West Central Great Plains SO AGRONOMY JOURNAL LA English DT Article ID DRYLAND WINTER-WHEAT; SOIL-WATER DEPLETION; GRAIN-SORGHUM; MANAGEMENT; NITROGEN; YIELD; SYSTEMS; EVAPOTRANSPIRATION; PROFITABILITY; SUNFLOWER AB Identifying the most profitable crop rotation for an area is a continuous research challenge. The objective of this study was to evaluate 2-, 3-, and 4-yr limited irrigation corn (Zea mays L.)-based crop rotations for grain yield, available soil water, crop water productivity, and profitability in comparison with 1 yr continuous corn (CC). A field study was conducted from 2001 through 2010 on a deep silt loam soil near Tribune, KS. The study consisted of four crop rotations, that is, CC, corn-winter wheat (Triticum aestivum L.) (C-W), corn-winter wheat-grain sorghum (Sorghum bicolor L.) (C-W-Gs), and corn-winter wheat-grain-sorghum-soybean [Glycine max (L.) Merr.] (C-W-Gs-Sb) with irrigation limited to 254 mm annually. Grain yield of corn from the CC rotation was significantly lower, on average by 2.5 Mg ha(-1), than corn yield from the other three rotations mainly due to difference in irrigation level. However, the mean yield of CC over one cycle of rotation [similar to 10.2 Mg (ha rotation-cycle)(-1)] was significantly greater than mean corn equivalent grain yield of the other three rotations over one cycle of each rotation. Seasonal soil water usage from upper depths of the soil profile was significantly greater for CC. In this study, average crop water productivity [16.6 kg (mm ha)(-1)] and profitability of the CC rotation were also greater than in the other rotations. In selecting profitable rotations, producers should consider crop yield potential and value in addition to resilience. C1 [Schlegel, Alan J.] Kansas State Univ, Southwest Res Extens Ctr, 1474 State Highway 96, Tribune, KS 67879 USA. [Assefa, Yared; Stone, Loyd R.; Thompson, Curtis R.] Kansas State Univ, Dept Agron, Throckmorton Hall, Manhattan, KS 66502 USA. [Dumler, Troy J.] Kansas State Univ, Southwest Area Extens Off, 4500 E Mary St, Garden City, KS 67846 USA. [Dumler, Troy J.] Garden City Co, Garden City, KS 67846 USA. [Haag, Lucas A.] Kansas State Univ, Northwest Res Extens Ctr, Colby, KS 67701 USA. [Halvorson, Ardell D.] USDA ARS, Ft Collins, CO 80526 USA. RP Schlegel, AJ (reprint author), Kansas State Univ, Southwest Res Extens Ctr, 1474 State Highway 96, Tribune, KS 67879 USA. EM schlegel@ksu.edu FU Kansas Corn Commission; Kansas Grain Sorghum Commission; Kansas Soybean Commission; Ogallala Aquifer Project of USDA-ARS [58-6209-6-031] FX This work was supported in part by funds from the Kansas Corn Commission, Kansas Grain Sorghum Commission, Kansas Soybean Commission, and the Ogallala Aquifer Project of USDA-ARS under Agreement no. 58-6209-6-031. This is Contribution 16-080-J, Kansas Agric. Exp. Stn. NR 42 TC 1 Z9 1 U1 3 U2 4 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2016 VL 108 IS 3 BP 1132 EP 1141 DI 10.2134/agronj2015.0536 PG 10 WC Agronomy SC Agriculture GA DO5DY UT WOS:000377805000021 ER PT J AU Hossard, L Archer, DW Bertrand, M Colnenne-David, C Debaeke, P Ernfors, M Jeuffroy, MH Munier-Jolain, N Nilsson, C Sanford, GR Snapp, SS Jensen, ES Makowski, D AF Hossard, Laure Archer, David W. Bertrand, Michel Colnenne-David, Caroline Debaeke, Philippe Ernfors, Maria Jeuffroy, Marie-Helene Munier-Jolain, Nicolas Nilsson, Chris Sanford, Gregg R. Snapp, Sieg S. Jensen, Erik S. Makowski, David TI A Meta-Analysis of Maize and Wheat Yields in Low-Input vs. Conventional and Organic Systems SO AGRONOMY JOURNAL LA English DT Article ID INTEGRATED WEED MANAGEMENT; CROPPING SYSTEMS; FARMING SYSTEMS; WINTER-WHEAT; PUBLICATION BIAS; NO-TILLAGE; SOIL; AGRICULTURE; INTENSIFICATION; PRODUCTIVITY AB Organic and low-input systems are proposed as ways to reduce the environmental impacts of agriculture. Previous studies have shown that yields of organic systems can be similar to 19 to 25% lower than conventional systems. An intermediary, low-input system could be less damaging for the environment than conventional systems, while reducing yield losses in comparison with organic systems. In this study, we performed a meta-analysis to compare low-input systems to conventional and organic systems. Our analysis is based on data of cropping system experiments conducted in Europe and North America, and focuses on two important crops, maize (Zea mays L.) and soft winter wheat (Triticum aestivum L.). Pesticide use was greatly reduced for low-input systems as compared with conventional for the two crops (50% for maize, 70% for wheat on average). Mean mineral N use was also reduced by 36% for maize and 28% for wheat in low-input relative to conventional. Maize yields in low-input systems were not different from those in conventional systems, and were higher than yields in organic systems (yield ratio of low-input vs. organic = 1.24). Wheat yields in low-input systems were lower than yields in conventional systems (yield ratio of low-input vs. conventional = 0.88), but were substantially higher than yields in organic systems (yield ratio of low input vs. organic = 1.43). This is one of the first meta-analyses to assess performance in terms of pesticide use intensity, and yields, with clear evidence emerging that low-input systems can markedly reduce pesticide application, without strongly reducing crop yields, relative to conventional systems. C1 [Hossard, Laure; Bertrand, Michel; Colnenne-David, Caroline; Jeuffroy, Marie-Helene; Makowski, David] INRA, Agron UMR211, F-78850 Thiverval Grignon, France. [Hossard, Laure; Bertrand, Michel; Colnenne-David, Caroline; Jeuffroy, Marie-Helene; Makowski, David] AgroParisTech, UMR Agron, F-78850 Thiverval Grignon, France. [Hossard, Laure] INRA, Innovat UMR0951, F-34000 Montpellier, France. [Archer, David W.] USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. [Debaeke, Philippe] INRA, AGIR UMR1248, F-31320 Castanet Tolosan, France. [Debaeke, Philippe] Univ Toulouse, INPT, UMR AGIR, F-31029 Toulouse, France. [Ernfors, Maria; Nilsson, Chris; Jensen, Erik S.] Swedish Univ Agr Sci, Dept Biosyst & Technol, SE-23053 Alnarp, Sweden. [Munier-Jolain, Nicolas] INRA, Agroecol UMR1347, F-21065 Dijon, France. [Munier-Jolain, Nicolas] AgroSup Dijon, UMR Agroecol, F-21065 Dijon, France. [Sanford, Gregg R.] Univ Wisconsin, Dept Agron, 1575 Linden Dr, Madison, WI 53706 USA. [Snapp, Sieg S.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Hossard, L (reprint author), INRA, Agron UMR211, F-78850 Thiverval Grignon, France.; Hossard, L (reprint author), AgroParisTech, UMR Agron, F-78850 Thiverval Grignon, France.; Hossard, L (reprint author), INRA, Innovat UMR0951, F-34000 Montpellier, France. EM laure.hossard@supagro.inra.fr OI Hossard, Laure/0000-0003-1117-9053 FU research program GloFoodS FX This work was partly funded by the research program GloFoodS. The authors thank Ben Boswell for English reviewing. NR 57 TC 2 Z9 2 U1 25 U2 53 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD MAY-JUN PY 2016 VL 108 IS 3 BP 1155 EP 1167 DI 10.2134/agronj2015.0512 PG 13 WC Agronomy SC Agriculture GA DO5DY UT WOS:000377805000023 ER PT J AU Smith, AD Yan, XH Chen, C Dawson, HD Bhagwat, AA AF Smith, Allen D. Yan, Xianghe Chen, Celine Dawson, Harry D. Bhagwat, Arvind A. TI Understanding the host-adapted state of Citrobacter rodentium by transcriptomic analysis SO ARCHIVES OF MICROBIOLOGY LA English DT Article DE Attaching-effacing pathogens; RNA-seq analysis; Host-adaptation; Enteropathogens ID ENTEROHEMORRHAGIC ESCHERICHIA-COLI; GENE-EXPRESSION DATA; PATHOGENICITY ISLAND; IN-VITRO; VIRULENCE; FIS; RNA; IDENTIFICATION; ELEMENTS; GROWTH AB Citrobacter rodentium (Cr) is a mouse pathogen that mimics many aspects of enteropathogenic Escherichia coli infections including producing attaching and effacing (A/E) lesions. Host-adapted (HA) Cr cells that are shed at the peak of infection have been reported to be hyper-infective. The exact mechanism underlying this phenomenon has remained elusive since the pathogen loses its HA 'status' immediately upon subculturing in laboratory media. We sequenced the entire transcriptome of Cr directly from the feces of infected mice and analyzed the gene expression pattern. We observed that the entire transcriptional machinery as well as several transcriptional regulators to be differentially expressed when compared with the transcriptome of cells grown on laboratory media. Major adhesion and effector genes, tir and eae, were highly expressed in HA along with many genes located on all five loci of enterocyte effacement regions (LEE 1-5). Notable absent among the HA expressed genes were 19 fimbrial operons and non-fimbrial adhesions and several non-LEE encoded effectors. These results demonstrate that host-adapted Cr has a unique transcriptome that is associated with increased host transmission. C1 [Smith, Allen D.; Chen, Celine; Dawson, Harry D.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA,BARC E, 10300 Baltimore Ave,B307C,Rm 228, Beltsville, MD 20705 USA. [Yan, Xianghe; Bhagwat, Arvind A.] ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. RP Smith, AD (reprint author), ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA,BARC E, 10300 Baltimore Ave,B307C,Rm 228, Beltsville, MD 20705 USA. EM allen.smith@ars.usda.gov NR 49 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0302-8933 EI 1432-072X J9 ARCH MICROBIOL JI Arch. Microbiol. PD MAY PY 2016 VL 198 IS 4 BP 353 EP 362 DI 10.1007/s00203-016-1191-y PG 10 WC Microbiology SC Microbiology GA DO7LC UT WOS:000377963000006 PM 26837900 ER PT J AU Zhang, DH Moreira, GSA Shoemaker, C Newton, JC Xu, DH AF Zhang, Dunhua Moreira, Gabriel S. A. Shoemaker, Craig Newton, Joseph C. Xu, De-Hai TI Detection and quantification of virulent Aeromonas hydrophila in channel catfish tissues following waterborne challenge SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE channel catfish; virulent Aeromonas hydrophila; waterborne challenge; adipose fin; tissue distribution; qPCR ID SALMON SALMO-SALAR; INFECTION; OUTBREAKS; DISEASE; SIZE AB The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus. Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a time course was detected and quantified using real-time polymerase chain reaction. The results showed that 77.8% of fish died within 48 h post challenge with mean day to death of 1.5 days. At 2 h post challenge, vAh (inferred from genomic DNA copies or genome equivalents) was detected in all external and internal tissues sampled. Gill had the highest vAh cells at 1 h post challenge. Spleen harbored the most vAh cells among internal organs at 4 h post challenge. The tissues/organs with most vAh cells detected at 8 h post challenge were adipose fin, blood, intestine, kidney and skin, while liver showed the highest vAh cells at 24 h post challenge. These results suggest that vAh was able to rapidly proliferate and spread, following wound infection, through the fish blood circulation system and cause mortality within 8-24 h. C1 [Zhang, Dunhua; Shoemaker, Craig; Xu, De-Hai] ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. [Moreira, Gabriel S. A.] Univ Sao Paulo, Lab Parasitol, Coll Anim Sci & Food Engn, Ave Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP, Brazil. [Newton, Joseph C.] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA. RP Zhang, DH (reprint author), ARS, USDA, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM dunhua.zhang@ars.usda.gov FU FAPESP [2015/01822-0]; United States Department of Agriculture/ARS CRIS Project [6010-32000-026-00D] FX We are also grateful to the FAPESP for providing a scholarship to Gabriel S. A. Moreira (Proc. no. 2015/01822-0). This research was supported by United States Department of Agriculture/ARS CRIS Project #6010-32000-026-00D. NR 21 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0378-1097 EI 1574-6968 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD MAY PY 2016 VL 363 IS 9 AR UNSP fnw080 DI 10.1093/femsle/fnw080 PG 5 WC Microbiology SC Microbiology GA DO7OA UT WOS:000377970700007 ER PT J AU Maloney, PE Eckert, AJ Vogler, DR Jensen, CE Mix, AD Neale, DB AF Maloney, Patricia E. Eckert, Andrew J. Vogler, Detlev R. Jensen, Camille E. Mix, Annette Delfino Neale, David B. TI Landscape Biology of Western White Pine: Implications for Conservation of a Widely-Distributed Five-Needle Pine at Its Southern Range Limit SO FORESTS LA English DT Article DE Cronartium ribicola; disease resistance; fire frequency; genetic diversity; historical logging; Pinus monticola; mountain pine beetle; population dynamics; upper montane; white pine blister rust ID ABIES-MAGNIFICA FORESTS; LAKE TAHOE BASIN; BLISTER RUST; SUGAR PINE; LAMBERTIANA DOUGL.; SIERRA-NEVADA; CASCADE RANGE; UNITED-STATES; FIRE HISTORY; TAEDA L. AB Throughout much of the range of western white pine, Pinus monticola Dougl., timber harvesting, fire exclusion and the presence of Cronartium ribicola J. C. Fisch., the white pine blister rust (WPBR) pathogen, have led to negative population and genetic consequences. To address these interactions, we examined population dynamics and genetic diversity in 10 populations of western white pine in upper montane forests of the Lake Tahoe Basin. We documented negative population trends for three of the 10 populations. These populations exhibited low estimated growth rates (), moderate to high incidences of WPBR and mountain pine beetle (MPB), and high levels of mortality. In contrast, seven populations appear to be stable ( 1.0), with low to moderate disease and insect incidence, and evidence for genetic resistance to WPBR. Genetic diversity (H-E) for a set of 160 single nucleotide polymorphisms was in the range of 0.245-0.272 across populations, and population-specific estimates of F-ST ranged from 0.0062 to 0.0244. Allele frequency of the Cr2 gene, which confers complete resistance to C. ribicola in western white pine, was low, averaging 0.009 for all populations sampled. However, a low frequency of pollen receptors (i.e., susceptible maternal parents pollinated by a local resistant parent) was found in nine of 10 populations. A moderate and negative relationship was found between the frequency of pollen receptors in a population and the incidence of WPBR (r(2) = 0.32). In the context of an introduced pathogen, climate driven outbreaks of MPB, fire exclusion, and prolonged drought, conservation and management strategies are warranted for this species in the Lake Tahoe Basin and likely other locations in California. These strategies include gene conservation of western white pine, WPBR resistance screening, and forest restoration treatments. C1 [Maloney, Patricia E.; Jensen, Camille E.] Univ Calif Davis, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA. [Maloney, Patricia E.; Jensen, Camille E.] Univ Calif Davis, Tahoe Environm Res Ctr, One Shields Ave, Davis, CA 95616 USA. [Eckert, Andrew J.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Vogler, Detlev R.; Mix, Annette Delfino] US Forest Serv, USDA, Pacific Southwest Res Stn, Inst Forest Genet, 2480 Carson Rd, Placerville, CA 95667 USA. [Neale, David B.] Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. RP Maloney, PE (reprint author), Univ Calif Davis, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA.; Maloney, PE (reprint author), Univ Calif Davis, Tahoe Environm Res Ctr, One Shields Ave, Davis, CA 95616 USA.; Eckert, AJ (reprint author), Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. EM pemaloney@ucdavis.edu; aeckert2@vcu.edu; dvogler@fs.fed.us; jensencamille@gmail.com; amix@fs.fed.us; dbneale@ucdavis.edu FU Southern Nevada Public Lands Management Act - USDA Forest Service, Pacific Southwest Research Station, Albany, CA FX We thank Tom Burt for field assistance and cone collections and Akiko Aguchi for GIS maps. We also thank Joan Dunlap, John Gleason, and Dessa Welty, USDA Forest Service, Sugar Pine Rust Resistance Program, Placerville Nursery, for processing western white pine cones and seed, seed-banking, and screening for Cr2. Jill Wegrzyn for bioinformatics and Katie Tsang for DNA extraction. David Fournier (USDA Forest Service, LTBMU), Roland Shaw (Nevada Division of Forestry), Woody Loftis (USDA, NRCS), Bill Champion (Nevada State Parks), and Audrey Newman (Heavenly Mountain Resort) for site information and permission to work on Federal, State, and private lands. Our work was supported by the Southern Nevada Public Lands Management Act-Rounds 7 and 9, which were sponsored by the USDA Forest Service, Pacific Southwest Research Station, Albany, CA. NR 59 TC 0 Z9 0 U1 7 U2 10 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD MAY PY 2016 VL 7 IS 5 AR 93 DI 10.3390/f7050093 PG 17 WC Forestry SC Forestry GA DO4ZQ UT WOS:000377793800002 ER PT J AU Chitwood, J Shie, AN Mou, BQ Evans, M Clark, J Motes, D Chen, PY Hensley, D AF Chitwood, Jessica Shie, Ainong Mou, Beiquan Evans, Michael Clark, John Motes, Dennis Chen, Pengyin Hensley, David TI Population Structure and Association Analysis of Bolting, Plant Height, and Leaf Erectness in Spinach SO HORTSCIENCE LA English DT Article ID GENOME-WIDE ASSOCIATION; GENETIC DIVERSITY; OLERACEA L.; MARKERS; RESISTANCE; TRAITS; STRATIFICATION; ACCESSIONS; GERMPLASM; SOFTWARE AB Spinach (Spinacia oleracea L.) is an important vegetable worldwide with high nutritional and health-promoting compounds. Bolting is an important trait to consider to grow spinach in different seasons and regions. Plant height and leaf erectness are important traits for machine harvesting. Breeding slow bolting, taller, and more erect spinach cultivars is needed for improved spinach production. A total of 288 United States Department of Agriculture (USDA) spinach accessions were used as the association panel in this research. Single-nucleotide polymorphisms (SNPs) discovered through genotyping by sequencing (GBS) were used for genotyping. Two structured populations and the admixtures were inferred for the 288 spinach accession panel using STRUCTURE and MEGA. Association mapping was conducted using single-marker regression (SMR) in QGene, and general linear model (GLM) and mixed linear model (MLM) built in TASSEL. Three SNP markers, AYZVO2001321_398, AYZVO2041012_1060, and AYZVO2118171_95 were identified to be associated with bolting. Eight SNP markers, AYZV02014270_540, AYZV02250508_2162, AYZV02091523_19842, AYZV02141794_376, AYZV02077023_64, AYZV02210662_2532, AYZV02153224_2197, and AYZV02003975_248 were found to be associated with plant height. Four SNP markers, AYZV02188832_229, AYZV02219088_79, AYZVO2030116_256, and AYZV02129827_197 were associated with erectness. These SNP markers may provide breeders with a tool in spinach molecular breeding to select spinach bolting, plant height, and erectness through marker-assisted selection (MAS). C1 [Chitwood, Jessica; Shie, Ainong; Evans, Michael; Clark, John; Hensley, David] Univ Arkansas, Dept Hort, 316 PTSC, Fayetteville, AR 72701 USA. [Mou, Beiquan] ARS, USDA, 1636 East Alisal St, Salinas, CA 93905 USA. [Motes, Dennis] Univ Arkansas, Vegetable Res Ctr, Alma, AR 72921 USA. [Chen, Pengyin] Univ Arkansas, Dept Crop Soil & Environm Sci, 215 PTSC, Fayetteville, AR 72701 USA. RP Shie, AN (reprint author), Univ Arkansas, Dept Hort, 316 PTSC, Fayetteville, AR 72701 USA.; Mou, BQ (reprint author), ARS, USDA, 1636 East Alisal St, Salinas, CA 93905 USA. EM ashi@uark.edu; Beiquan.Mou@ars.usda.gov FU USDA National Institute of Food and Agriculture Hatch project [1002423] FX This work is supported, at least in part, by the USDA National Institute of Food and Agriculture Hatch project accession number 1002423. NR 40 TC 0 Z9 0 U1 6 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2016 VL 51 IS 5 BP 481 EP 486 PG 6 WC Horticulture SC Agriculture GA DO5TU UT WOS:000377846500003 ER PT J AU Gerovac, JR Craver, JK Boldt, JK Lopez, RG AF Gerovac, Joshua R. Craver, Joshua K. Boldt, Jennifer K. Lopez, Roberto G. TI Light Intensity and Quality from Sole-source Light-emitting Diodes Impact Growth, Morphology, and Nutrient Content of Brassica Microgreens SO HORTSCIENCE LA English DT Article ID SPROUTING BROCCOLI; RED LIGHT; GREEN; BLUE AB Multilayer vertical production systems using sole-source (SS) lighting can be used for the production of microgreens; however, traditional SS lighting methods can consume large amounts of electrical energy. Light-emitting diodes (LEDs) offer many advantages over conventional light sources, including high photoelectric conversion efficiencies, narrowband spectral light quality (LQ), low thermal output, and adjustable light intensifies (LIs). The objective of this study was to quantify the effects of SS LEDs of different light qualities and intensities on growth, morphology, and nutrient content of Brassica microgreens. Purple kohlrabi (Brassica oleracea L. var. gongylodes L.), mizuna (Brassica rapa L. var. japonica), and mustard [Brassica juncea (L.) Czern. 'Garnet Giant'] were grown in hydroponic tray systems placed on multilayer shelves in a walk-in growth chamber. A daily light integral (DLI) of 6, 12, or 18 mol.m(-2).d(-1) was achieved from commercially available SS LED arrays with light ratios (%) of red:green:blue 74:18:8 (R-74:G(18):B-8), red:blue 87:13 (R-87:B-13), or red:far-red:blue 84:7:9 (R-84:FR7:B-9) with a total photon flux (TPF) from 400 to 800 nm of 105, 210, or 315 mu mol.m(-2).s(-1) for 16 hours. Regardless of LQ, as the LI increased from 105 to 315 mu mol.m(-2).s(-1), hypocotyl length (HL) decreased and percent dry weight (DW) increased for kohlrabi, mizuna, and mustard microgreens. With increasing LI, leaf area (LA) of kohlrabi generally decreased and relative chlorophyll content (RCC) increased. In addition, nutrient content increased under low LIs regardless of LQ. The results from this study can help growers to select LIs and LQs from commercially available SS LEDs to achieve preferred growth characteristics of Brassica microgreens. C1 [Gerovac, Joshua R.; Craver, Joshua K.] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Boldt, Jennifer K.] USDA ARS, Greenhouse Prod Res Grp, 2801 W Bancroft St,Mail Stop 604, Toledo, OH 43606 USA. [Lopez, Roberto G.] Michigan State Univ, Dept Hort, 1066 Bogue St,Room A288, E Lansing, MI 48824 USA. RP Lopez, RG (reprint author), Michigan State Univ, Dept Hort, 1066 Bogue St,Room A288, E Lansing, MI 48824 USA. EM rglopez@msu.edu FU USDA-NIFA SCRI [2010-51181-21369]; Philips Lighting; Hort Americas for LED lighting FX The USDA-NIFA SCRI grant no. 2010-51181-21369 for funding and Philips Lighting and Hort Americas for LED lighting. NR 21 TC 1 Z9 1 U1 32 U2 39 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2016 VL 51 IS 5 BP 497 EP 503 PG 7 WC Horticulture SC Agriculture GA DO5TU UT WOS:000377846500005 ER PT J AU Rickard, BJ Rudell, DR Watkins, CB AF Rickard, Bradley J. Rudell, David R. Watkins, Christopher B. TI Ex ante Economic Evaluation of Technologies for Managing Postharvest Physiological Disorders: The Case of 'Empire' Apples in New York State SO HORTSCIENCE LA English DT Article ID CONTROLLED-ATMOSPHERE AB Recently there has been much progress in the development of technologies that use biomarkers to detect and manage postharvest physiological disorders for apples in long-term storage. Such technologies have the capacity to alleviate fruit loss by allowing storage operators to more effectively manage the disorder by adjusting stock distribution. The technology may also reduce costs for storage materials and associated management activities. However, as is common for many new technologies that have not yet been adopted commercially in agriculture, the net economic value of the technology is not well understood and is difficult to assess ex ante. In horticultural markets that include quality (and price) differentiated products, technologies that affect grading are expected to impact revenues in nontrivial ways. Here we develop a framework to assess the likely range of economic implications associated with the adoption of the biomarker technology that allows a greater share of fruit to be marketed in a higher grade and may influence the costs of storing fruit. Results indicate that 10% increases in the share of higher quality fruit lead to increased profits of between 0.99% and 3%. A scenario that leads to a 10% increase in the share of fruit in higher grades and a 10% decrease in material costs for storage would increase profits by approximate to 4.4%. Our analysis and results are specific to the case of biomarker use to manage postharvest disorders for 'Empire' [Malus sylvestris (L.) Mill var. domestica Borkh.] apples, yet the framework can be used with cultivar-specific price and yield information to assess the ex ante economic implications of adopting the technology more generally. C1 [Rickard, Bradley J.] Cornell Univ, Charles H Dyson Sch Appl Econ & Management, Ithaca, NY 14853 USA. [Rudell, David R.] USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. [Watkins, Christopher B.] Cornell Univ, Sch Integrat Plant Sci, Hort Sect, Ithaca, NY 14853 USA. RP Rickard, BJ (reprint author), Cornell Univ, Charles H Dyson Sch Appl Econ & Management, Ithaca, NY 14853 USA. EM b.rickard@cornell.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2010-51181-21446]; U.S. Department of Agriculture [58-3000-3-0021] FX This material is based on the work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number 2010-51181-21446 and by U.S. Department of Agriculture Cooperative Agreement number 58-3000-3-0021. NR 14 TC 0 Z9 0 U1 4 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2016 VL 51 IS 5 BP 537 EP 542 PG 6 WC Horticulture SC Agriculture GA DO5TU UT WOS:000377846500011 ER PT J AU Trigiano, RN Windham, AS Windham, MT Wadl, PA AF Trigiano, Robert N. Windham, Alan S. Windham, Mark T. Wadl, Phillip A. TI 'Appalachian Joy' is a Supernumerary, White-bracted Cultivar of Flowering Dogwood (Cornus florida) Resistant to Powdery Mildew (Erysiphe pulchra) SO HORTSCIENCE LA English DT Article ID DISCULA-DESTRUCTIVA; NORTH-AMERICA; ANTHRACNOSE C1 [Trigiano, Robert N.; Windham, Mark T.; Wadl, Phillip A.] Univ Tennessee, Dept Entomol & Plant Pathol, 2505 EJ Chapman Dr,370 Plant Biotechnol Bldg, Knoxville, TN 37996 USA. [Windham, Alan S.] Univ Tennessee, Dept Entomol & Plant Pathol, 5201 Marchant Dr, Nashville, TN 37211 USA. [Wadl, Phillip A.] ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. RP Trigiano, RN (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, 2505 EJ Chapman Dr,370 Plant Biotechnol Bldg, Knoxville, TN 37996 USA. EM rtrigian@utk.edu FU United States Department of Agriculture [58-6404-1637]; AgResearch at the Tennessee Institute of Agriculture FX This work was supported by the United States Department of Agriculture (grant no. 58-6404-1637) and AgResearch at the Tennessee Institute of Agriculture. NR 16 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2016 VL 51 IS 5 BP 592 EP 594 PG 3 WC Horticulture SC Agriculture GA DO5TU UT WOS:000377846500020 ER PT J AU Scorza, R Ravelonandro, M Callahan, A Zagrai, I Polak, J Malinowski, T Cambra, M Levy, L Damsteegt, V Krska, B Cordts, J Gonsalves, D Dardick, C AF Scorza, Ralph Ravelonandro, Michel Callahan, Ann Zagrai, Ioan Polak, Jaroslav Malinowski, Tadeuz Cambra, Mariano Levy, Laurene Damsteegt, Vern Krska, Boris Cordts, John Gonsalves, Dennis Dardick, Chris TI 'HoneySweet' (C5), the First Genetically Engineered Plum pox virus-resistant Plum (Prunus domestica L.) Cultivar SO HORTSCIENCE LA English DT Article ID TRANSGENIC PLUMS; FIELD; GENE; PPV; INOCULATION; INFECTION; APHID C1 [Scorza, Ralph; Callahan, Ann; Dardick, Chris] ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Ravelonandro, Michel] INRA Bordeaux, UMR BFP 1332, Unite Mixte Rech Biol Fruit & Pathol, 71 Ave Bourlaux, F-33882 Villenave Dornon, France. [Zagrai, Ioan] Statiunea Cercetare Dezvoltare Pentru Pomicultura, Drumul Dumitrei Nou 3, Bistrita 420127, Judetul Bistrit, Romania. [Polak, Jaroslav] Res Inst Crop Prod, Dept Virol & Phytoplasmatol, Drnovska 507, Prague 16105, Czech Republic. [Malinowski, Tadeuz] Inst Hort Res, Pomologiczna 18, PL-96100 Skierniewice, Poland. [Cambra, Mariano] IVIA, Plant Protect & Biotechnol Ctr, Carretera Moncada Naquera Km 5, Moncada 46113, Spain. [Levy, Laurene] Anim & Plant Hlth Inspect Serv, USDA, Plant Protect & Quarantine, 4700 River Rd,Unit 152,5C-03-50, Riverdale, MD 20737 USA. [Damsteegt, Vern] ARS, USDA, Foreign Dis Weed Sci Res, Ft Detrick, MD USA. [Krska, Boris] Mendel Univ Brno, DFG FH, Dept Fruit Growing, Valticka 337, Lednice 69144, Czech Republic. [Cordts, John] Cordts Consulting LLC, Newark, DE USA. [Gonsalves, Dennis] ARS, USDA, Daniel K Inouye US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Scorza, R (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM ralph.scorza@ars.usda.gov RI Cambra Alvarez, Mariano/O-6367-2014 OI Cambra Alvarez, Mariano/0000-0003-2170-9521 NR 22 TC 1 Z9 1 U1 3 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD MAY PY 2016 VL 51 IS 5 BP 601 EP 603 PG 3 WC Horticulture SC Agriculture GA DO5TU UT WOS:000377846500023 ER PT J AU Srygley, RB Lorch, PD AF Srygley, Robert B. Lorch, Patrick D. TI Loss of safety in numbers and a novel driver of mass migration: radiotelemetry reveals heavy wasp predation on a band of Mormon crickets SO ROYAL SOCIETY OPEN SCIENCE LA English DT Article DE parasitism; digger wasp; numerical response; Orthoptera; katydid; biological control ID ANABRUS-SIMPLEX; RISK; TETTIGONIIDAE; ORTHOPTERA; IMMUNITY; PROTEIN; LOCUSTS; POPULATIONS; INFECTION; CONFUSION AB Coordinated movement of animals is a spectacular phenomenon that has received much attention. Experimental studies of Mormon crickets and locust nymphs have demonstrated that collective motion can arise from cannibalism that compensates for nutritional deficiencies arising from group living. Grouping into migratory bands confers protection from predators. By radiotracking migrating, Mormon crickets released over 3 days, we found that specialized, parasitoid digger wasps (Sphecidae) respond numerically and prey heavily on aggregated Mormon crickets leading to loss of safety in numbers. Palmodes laeviventris paralysed and buried 42% of tagged females and 8% of the males on the final day of tracking. Risk of wasps and Mormon crickets hatching on the same site is high and may drive nymphal emigration. A preference to provision offspring with adult female Mormon crickets can be explained by their greater fat content and larger size compared with males, improving survival of wasps during diapause. C1 [Srygley, Robert B.] ARS, Northern Plains Agr Res Lab, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. [Lorch, Patrick D.] Kent State Univ, Dept Biol Sci, Kent, OH 44242 USA. [Lorch, Patrick D.] Cleveland Metropk, 2277 West Ridgewood Dr, Parma, OH 44134 USA. RP Srygley, RB (reprint author), ARS, Northern Plains Agr Res Lab, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. EM robert.srygley@ars.usda.gov NR 38 TC 1 Z9 1 U1 4 U2 4 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 2054-5703 J9 ROY SOC OPEN SCI JI R. Soc. Open Sci. PD MAY PY 2016 VL 3 IS 5 AR UNSP 160113 DI 10.1098/rsos.160113 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DO7NR UT WOS:000377969800016 PM 27293791 ER PT J AU Reddy, GB Raczkowski, CW Cyrus, JS Szogi, A AF Reddy, Gudigopuram B. Raczkowski, Charles W. Cyrus, Johnsely S. Szogi, Ariel TI Carbon sequestration in a surface flow constructed wetland after 12 years of swine wastewater treatment SO WATER SCIENCE AND TECHNOLOGY LA English DT Article DE carbon sequestration; constructed wetlands; swine wastewater ID SOIL ORGANIC-MATTER; NEW-ZEALAND; POOLS; NITROGEN; DENITRIFICATION; DECOMPOSITION; TEMPERATURE; DYNAMICS; BALANCE; STORAGE AB Constructed wetlands used for the treatment of swine wastewater may potentially sequester significant amounts of carbon. In past studies, we evaluated the treatment efficiency of wastewater in a marsh-pond-marsh design wetland system. The functionality of this system was highly dependent on soil carbon content and organic matter turnover rate. To better understand system performance and carbon dynamics, we measured plant dry matter, decomposition rates and soil carbon fractions. Plant litter decomposition rate was 0.0052 g day(-1) (+/- 0.00119 g day(-1)) with an estimated half-life of 133 days. The detritus layer accumulated over the soil surface had much more humin than other C fractions. In marsh areas, soil C extracted with NaOH had four to six times higher amounts of humic acid, fulvic acid and humin than soil C extracted by cold and hot water, HCl/HF, and Na pyruvate. In the pond area, humic acid, fulvic acid and humin content were two to four times lower than in the marsh area. More soil C and N was found in the marsh area than in the pond area. These wetlands proved to be large sinks for stable C forms. C1 [Reddy, Gudigopuram B.; Raczkowski, Charles W.; Cyrus, Johnsely S.] N Carolina Agr & Tech State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA. [Szogi, Ariel] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Cyrus, JS (reprint author), N Carolina Agr & Tech State Univ, Dept Nat Resources & Environm Design, Greensboro, NC 27411 USA. EM jscyrus@ncat.edu FU Evans-Allen/USDA-NIFA Grant [NCX-259-5-11-130-1] FX This work was financially supported by the Evans-Allen/USDA-NIFA Grant, NCX-259-5-11-130-1. NR 31 TC 0 Z9 0 U1 15 U2 22 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 0273-1223 EI 1996-9732 J9 WATER SCI TECHNOL JI Water Sci. Technol. PD MAY PY 2016 VL 73 IS 10 BP 2501 EP 2508 DI 10.2166/wst.2016.112 PG 8 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DO4MT UT WOS:000377757900024 PM 27191573 ER PT J AU Nghiem, NP Montanti, J Kim, TH AF Nghiem, Nhuan P. Montanti, Justin Kim, Tae Hyun TI Pretreatment of Dried Distiller Grains with Solubles by Soaking in Aqueous Ammonia and Subsequent Enzymatic/Dilute Acid Hydrolysis to Produce Fermentable Sugars SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE Ethanol fermentation; Dried distillers grains with solubles; Soaking in aqueous ammonia pretreatment; Dilute acid/enzyme hydrolysis; Succinic acid ID LIQUID HOT-WATER; SUCCINIC ACID; ETHANOL-PRODUCTION; CORN STOVER; AFEX; FRACTIONATION; CONVERSION AB Dried distillers grains with solubles (DDGS), a co-product of corn ethanol production in the dry-grind process, was pretreated by soaking in aqueous ammonia (SAA) using a 15 % w/w NH4OH solution at a solid/liquid ratio of 1:10. The effect of pretreatment on subsequent enzymatic hydrolysis was studied at two temperatures (40 and 60 A degrees C) and four reaction times (6, 12, 24, and 48 h). Highest glucose yield of 91 % theoretical was obtained for the DDGS pretreated at 60 A degrees C and 24 h. The solubilized hemicellulose in the liquid fraction was further hydrolyzed with dilute H2SO4 to generate fermentable monomeric sugars. The conditions of acid hydrolysis included 1 and 4 wt% acid, 60 and 120 A degrees C, and 0.5 and 1 h. Highest yields of xylose and arabinose were obtained at 4 wt% acid, 120 A degrees C, and 1 h. The fermentability of the hydrolysate obtained by enzymatic hydrolysis of the SAA-pretreated DDGS was demonstrated in ethanol fermentation by Saccharomyces cerevisiae. The fermentability of the hydrolysate obtained by consecutive enzymatic and dilute acid hydrolysis was demonstrated using a succinic acid-producing microorganism, strain Escherichia coli AFP184. Under the fermentation conditions, complete utilization of glucose and arabinose was observed, whereas only 47 % of xylose was used. The succinic acid yield was 0.60 g/g total sugar consumed. C1 [Nghiem, Nhuan P.; Montanti, Justin] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Kim, Tae Hyun] Kongju Natl Univ, Dept Environm Engn, Cheonandae Ro 1223-24, Cheonan 31080, Chungnam, South Korea. RP Nghiem, NP (reprint author), ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM john.nghiem@ars.usda.gov FU R&D program of MOTIE/KETEP [20153010091990] FX The authors would like to express their sincere thanks to Mr. Gerard Senske of the ERRC, who assisted in the experimental efforts and sample analysis, DuPont Industrial Biosciences for providing the enzyme products, Lincolnway Energy, LLC for providing the DDGS, and Mr. Kwang Ho Kim at the Department of Agricultural and Biosystems Engineering, Iowa State University for assisting in obtaining the DDGS. Tae Hyun Kim was supported by the R&D program of MOTIE/KETEP ( No. 20153010091990). NR 30 TC 0 Z9 0 U1 5 U2 10 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 EI 1559-0291 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD MAY PY 2016 VL 179 IS 2 BP 237 EP 250 DI 10.1007/s12010-016-1990-2 PG 14 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DO0JO UT WOS:000377465400004 PM 26797927 ER PT J AU Barnes, ML Moran, MS Scott, RL Kolb, TE Ponce-Campos, GE Moore, DJP Ross, MA Mitra, B Dore, S AF Barnes, Mallory L. Moran, M. Susan Scott, Russell L. Kolb, Thomas E. Ponce-Campos, Guillermo E. Moore, David J. P. Ross, Morgan A. Mitra, Bhaskar Dore, Sabina TI Vegetation productivity responds to sub-annual climate conditions across semiarid biomes SO ECOSPHERE LA English DT Article DE aboveground net primary production; drought; eddy covariance; Enhanced Vegetation Index (EVI); forests; global change; grasslands; gross ecosystem production; Moderate-resolution Imaging Spectroradiometer; shrublands; US Southwest ID TERRESTRIAL ECOSYSTEM PRODUCTION; SOUTHWESTERN NORTH-AMERICA; EARLY 21ST-CENTURY DROUGHT; GRASSLAND PRODUCTIVITY; TEMPORAL DYNAMICS; UNITED-STATES; DIE-OFF; WATER; VARIABILITY; IMPACTS AB In the southwest United States, the current prolonged warm drought is similar to the predicted future climate change scenarios for the region. This study aimed to determine patterns in vegetation response to the early 21st century drought across multiple biomes. We hypothesized that different biomes (forests, shrublands, and grasslands) would have different relative sensitivities to both climate drivers (precipitation and temperature) and legacy effects (previous-year's productivity). We tested this hypothesis at eight Ameriflux sites in various Southwest biomes using NASA Moderate-resolution Imaging Spectroradiometer Enhanced Vegetation Index (EVI) from 2001 to 2013. All sites experienced prolonged dry conditions during the study period. The impact of combined precipitation and temperature on Southwest ecosystems at both annual and sub-annual timescales was tested using Standardized Precipitation Evapotranspiration Index (SPEI). All biomes studied had critical sub-annual climate periods during which precipitation and temperature influenced production. In forests, annual peak greenness (EVImax) was best predicted by 9-month SPEI calculated in July (i.e., January-July). In shrublands and grasslands, EVImax was best predicted by SPEI in July through September, with little effect of the previous year's EVImax. Daily gross ecosystem production (GEP) derived from flux tower data yielded further insights into the complex interplay between precipitation and temperature. In forests, GEP was driven by cool-season precipitation and constrained by warm-season maximum temperature. GEP in both shrublands and grasslands was driven by summer precipitation and constrained by high daily summer maximum temperatures. In grasslands, there was a negative relationship between temperature and GEP in July, but no relationship in August and September. Consideration of sub-annual climate conditions and the inclusion of the effect of temperature on the water balance allowed us to generalize the functional responses of vegetation to predicted future climate conditions. We conclude that across biomes, drought conditions during critical sub-annual climate periods could have a strong negative impact on vegetation production in the southwestern United States. C1 [Barnes, Mallory L.; Moore, David J. P.; Ross, Morgan A.; Mitra, Bhaskar] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85719 USA. [Moran, M. Susan; Scott, Russell L.; Ponce-Campos, Guillermo E.] ARS, USDA, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Kolb, Thomas E.; Dore, Sabina] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86001 USA. RP Barnes, ML (reprint author), Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85719 USA. EM mallorybarnes@email.arizona.edu FU NASA SMAP Science Definition Team [08-SMAPSDT08-0042]; NASA SMAP Science Team [NNH14AX72I]; U.S. Department of Energy's Office of Science FX The research was partly funded by the NASA SMAP Science Definition Team under agreement 08-SMAPSDT08-0042, and the NASA SMAP Science Team under agreement NNH14AX72I. We also acknowledge Dr. Marcy Litvak, who contributed tower data for the US-Vcm, US-Vcp, US-Ses, and US-Seg towers to the AmeriFlux data archive (http://ameri-flux.ornl.gov/). Funding for AmeriFlux core site data and data resources was provided by the U.S. Department of Energy's Office of Science. This manuscript was greatly improved by comments from David D. Breshears and Joel A. Biederman and we thank them for their contributions. NR 58 TC 2 Z9 2 U1 10 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2016 VL 7 IS 5 AR e01339 DI 10.1002/ecs2.1339 PG 20 WC Ecology SC Environmental Sciences & Ecology GA DN6VS UT WOS:000377215200034 ER PT J AU Latif, QS Sanderlin, JS Saab, VA Block, WM Dudley, JG AF Latif, Quresh S. Sanderlin, Jamie S. Saab, Victoria A. Block, William M. Dudley, Jonathan G. TI Avian relationships with wildfire at two dry forest locations with different historical fire regimes SO ECOSPHERE LA English DT Article DE Arizona; burn severity; disturbance; dry conifer forests; fire regimes; forest bird communities; habitat management; Idaho; multispecies occupancy models; population responses; species richness; wildfire ID PONDEROSA PINE FORESTS; MIXED-CONIFER FORESTS; CAVITY-NESTING BIRDS; DISTURBANCE REGIMES; DOMINATED FORESTS; SPECIES RICHNESS; CLIMATE-CHANGE; BURN SEVERITY; POINT COUNTS; COMMUNITIES AB Wildfire is a key factor influencing bird community composition in western North American forests. We need to understand species and community responses to wildfire and how responses vary regionally to effectively manage dry conifer forests for maintaining biodiversity. We compared avian relationships with wildfire burn severity between two dry forest locations of Arizona and Idaho. We predicted different responses to wildfire between locations due to regional differences in historical fire regime. We conducted point count surveys for 3 yr following wildfire (Arizona: 1997-1999; Idaho: 2008-2010) and used multispecies hierarchical models to analyze relationships of bird occupancy with burn severity. Consistent with our prediction for mixed-severity fire regimes characterizing the Idaho location, we observed proportionately more positive species occupancy relationships and, consequently, a positive species richness relationship with burn severity in Idaho. We also observed the opposite pattern in Arizona, which was congruent with our prediction for the low-severity fire regime characterizing that location. Cavity nesters and aerial insectivores occupied more severely burned sites following wildfire, corresponding with predicted increases in nesting substrate and foraging opportunities for these species. In contrast, canopy-nesting foliage gleaners and pine seed consumers exhibited negative relationships with burn severity. Our results were consistent with predictions based on species life histories and with patterns from the literature, suggesting generality of observed relationships and locational difference in relationships with wildfire. We therefore suggest that optimal management strategies for maintaining avian diversity could differ regionally. Specifically, intensive fuels management may be ecologically less appropriate for promoting biodiversity in areas such as the Idaho location where mixed-severity wildfires and dense forest stands were historically more common. C1 [Latif, Quresh S.; Saab, Victoria A.] US Forest Serv, Rocky Mt Res Stn, Bozeman, MT 59717 USA. [Sanderlin, Jamie S.; Block, William M.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Dudley, Jonathan G.] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. RP Latif, QS (reprint author), US Forest Serv, Rocky Mt Res Stn, Bozeman, MT 59717 USA. EM qlatif@fs.fed.us FU National Fire Plan; Payette National Forest; Coconino National Forest FX National Fire Plan, Payette National Forest, and Coconino National Forest provided funding and logistical support for this work. We thank field crews for collecting avian data. In particular, J. Dwyer, B. Strohmeyer, K. Bratland, T. Pope, L. Doll, L. McGrath, L. Dickson, G. Martinez, J. Iniguez, and S. Vojta helped with fieldwork on the Coconino study area, and A. Newhouse, D. Ramos, S. Story, and S. Copeland helped with field work on the Payette study area. A. White, B. Bird, S. Haire, and 1-2 anonymous reviewers provided helpful comments on earlier drafts of this manuscript. NR 88 TC 1 Z9 1 U1 12 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2016 VL 7 IS 5 DI 10.1002/ecs2.1346 PG 22 WC Ecology SC Environmental Sciences & Ecology GA DN6VS UT WOS:000377215200039 ER PT J AU Nitzsche, KN Verch, G Premke, K Gessler, A Kayler, ZE AF Nitzsche, Kai Nils Verch, Gernot Premke, Katrin Gessler, Arthur Kayler, Zachary E. TI Visualizing land-use and management complexity within biogeochemical cycles of an agricultural landscape SO ECOSPHERE LA English DT Article DE agricultural landscape; isoscape; land management; land-use change and impacts; spatial visualization; stable isotopes ID WATER-USE EFFICIENCY; SOIL ORGANIC-CARBON; ISOTOPIC COMPOSITION; NATURAL-ABUNDANCE; CLIMATE-CHANGE; PLANT CARBON; NITROGEN; N-15; ECOSYSTEMS; GRADIENT AB Crop fields are cultivated across continuities of soil, topography, and local climate that drive biological processes and nutrient cycling at the landscape scale; yet land management and agricultural research are often performed at the field scale, potentially neglecting the context of the surrounding landscape. Adding to this complexity is the overlap of ecosystems and their biogeochemical legacies, as a patchwork of crops fields, natural grasslands, and forests develops across the landscape. Furthermore, as new technologies and policies are introduced, management practices change, including fertilization strategies, which further alter biological productivity and nutrient cycling. All of these environmental, biological, and historical legacies are potentially recorded in the isotopic signal of plant, soil, and sediment organic matter across the landscape. We mapped over 1500 plant, soil, and sediment isotopic values and generated an isotopic landscape (isoscape) over a 40-km(2) agricultural site in NE Germany. We observed distinct patterns in the isotopic composition of organic matter sampled from the landscape that clearly reflect the landscape complexity. C-3 crop intrinsic water-use efficiency reflected a precipitation gradient, while native forest and grassland plant species did not, suggesting that native plants are more adapted to predominant climatic conditions. delta C-13(soil) patterns reflected both the long-term input of plant organic matter, which was affected by the local climate conditions, and the repeated cultivation of corn. Soil organic matter N-15 isotopic values also revealed spatial differences in fertilization regimes. Forest fragments, in which the nitrogen cycle was relatively open, were more water-use efficient. Sediments from small water bodies received substantial inputs from surrounding field vegetation but were also affected by seasonal drying. These isotopic maps can be used to visualize large spatial heterogeneity and complexity, and they are a powerful means to interpret past and current trends in agricultural landscapes. C1 [Nitzsche, Kai Nils; Premke, Katrin; Gessler, Arthur; Kayler, Zachary E.] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Eberswalder Str 84, D-15374 Muncheberg, Germany. [Verch, Gernot] Leibniz Ctr Agr Landscape Res ZALF, Res Stn, Dedelow, Steinfurther Str 14, D-17291 Prenzlau, Germany. [Premke, Katrin] Leibniz Inst Freshwater Ecol & Inland Fisheries, Mueggelseedamm 310, D-12587 Berlin, Germany. [Gessler, Arthur] Swiss Fed Inst Forest Snow & Landscape Res WSL, Zuercherstr 111, CH-8903 Birmensdorf, Switzerland. [Gessler, Arthur] Berlin Brandenburg Inst Adv Biodivers Res BBIB, D-14195 Berlin, Germany. [Kayler, Zachary E.] Lawrence Livermore Natl Lab, USDA Forest Serv, No Res Stn, Livermore, CA 94550 USA. RP Kayler, ZE (reprint author), Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, Eberswalder Str 84, D-15374 Muncheberg, Germany.; Kayler, ZE (reprint author), Lawrence Livermore Natl Lab, USDA Forest Serv, No Res Stn, Livermore, CA 94550 USA. EM kayler3@llnl.gov RI Gessler, Arthur/C-7121-2008 OI Gessler, Arthur/0000-0002-1910-9589 FU Pact for Innovation and Research of the Gottfried Wilhelm Leibniz association (project LandScales) FX We thank the Research Station of ZALF at Dedelow for the logistical support and Frau Remus and Thomas Wagner for their help with the sample preparation. We kindly thank the LandScales team for their support and discussions including Thomas Kalettka for assistance with kettle hole selection. This research was funded through the Pact for Innovation and Research of the Gottfried Wilhelm Leibniz association (project LandScales, http://landscales.de). NR 62 TC 1 Z9 1 U1 11 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2016 VL 7 IS 5 AR e01282 DI 10.1002/ecs2.1282 PG 16 WC Ecology SC Environmental Sciences & Ecology GA DN6VS UT WOS:000377215200015 ER PT J AU Siers, SR Reed, RN Savidge, JA AF Siers, Shane R. Reed, Robert N. Savidge, Julie A. TI To cross or not to cross: modeling wildlife road crossings as a binary response variable with contextual predictors SO ECOSPHERE LA English DT Article DE animal behavior; Boiga irregularis; Guam; invasive species; mixed effects logistic regression; movement ecology; radiotelemetry; road crossing avoidance; road ecology ID TREESNAKES BOIGA-IRREGULARIS; BROWN TREE SNAKE; MALE-BIASED DISPERSAL; HOME-RANGE SIZE; HABITAT USE; BEHAVIORAL-RESPONSES; EASTERN MASSASAUGA; SPATIAL ECOLOGY; STEGONOTUS-CUCULLATUS; REPRODUCTIVE-BIOLOGY AB Roads are significant barriers to landscape-scale movements of individuals or populations of many wildlife taxa. The decision by an animal near a road to either cross or not cross may be influenced by characteristics of the road, environmental conditions, traits of the individual animal, and other aspects of the context within which the decision is made. We considered such factors in a mixed-effects logistic regression model describing the nightly road crossing probabilities of invasive nocturnal Brown Treesnakes (Boiga irregularis) through short-term radiotracking of 691 snakes within close proximity to 50 road segments across the island of Guam. All measures of road magnitude (traffic volume, gap width, surface type, etc.) were significantly negatively correlated with crossing probabilities. Snake body size was the only intrinsic factor associated with crossing rates, with larger snakes crossing roads more frequently. Humidity was the only environmental variable affecting crossing rate. The distance of the snake from the road at the start of nightly movement trials was the most significant predictor of crossings. The presence of snake traps with live mouse lures during a portion of the trials indicated that localized prey cues reduced the probability of a snake crossing the road away from the traps, suggesting that a snake's decision to cross roads is influenced by local foraging opportunities. Per capita road crossing rates of Brown Treesnakes were very low, and comparisons to historical records suggest that crossing rates have declined in the 60+ yr since introduction to Guam. We report a simplified model that will allow managers to predict road crossing rates based on snake, road, and contextual characteristics. Road crossing simulations based on actual snake size distributions demonstrate that populations with size distributions skewed toward larger snakes will result in a higher number of road crossings. Our method of modeling per capita road crossing probabilities as a binary response variable, influenced by contextual factors, may be useful for describing or predicting road crossings by individuals of other taxa provided that appropriate spatial and temporal resolution can be achieved and that potentially influential covariate data can be obtained. C1 [Siers, Shane R.; Savidge, Julie A.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Reed, Robert N.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Siers, Shane R.] USDA, APHIS, WS Natl Wildlife Res Ctr, Hawaii Field Stn, Hilo, HI 96720 USA. RP Siers, SR (reprint author), Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA.; Siers, SR (reprint author), USDA, APHIS, WS Natl Wildlife Res Ctr, Hawaii Field Stn, Hilo, HI 96720 USA. EM shane.r.siers@aphis.usda.gov FU U.S. Department of the Interior's Office of Insular Affairs via the U.S. Geological Survey FX Funding for this project was provided by the U.S. Department of the Interior's Office of Insular Affairs via the U.S. Geological Survey. Site access was facilitated by S. Mosher. M. Mazurek coordinated the assistance of field staff. M. Cook was particularly helpful with field activities. Administrative and logistical support were provided by L. Bonewell and G. Engler. E. Guinness of the Washington University Department of Earth and Planetary Sciences provided guidance on acquiring and interpreting moonlight data. D. Ouren, U.S. Geological Survey, provided traffic monitoring equipment and guidance. All animal use was conducted in accordance with Colorado State University IACUC Protocol #12-3271A. Any use of trade, product or firm name is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 98 TC 0 Z9 0 U1 15 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2016 VL 7 IS 5 AR e01292 DI 10.1002/ecs2.1292 PG 19 WC Ecology SC Environmental Sciences & Ecology GA DN6VS UT WOS:000377215200019 ER PT J AU Weise, L Ulrich, A Moreano, M Gessler, A Kayler, ZE Steger, K Zeller, B Rudolph, K Knezevic-Jaric, J Premke, K AF Weise, Lukas Ulrich, Andreas Moreano, Matilde Gessler, Arthur Kayler, Zachary E. Steger, Kristin Zeller, Bernd Rudolph, Kristin Knezevic-Jaric, Jelena Premke, Katrin TI Water level changes affect carbon turnover and microbial community composition in lake sediments SO FEMS MICROBIOLOGY ECOLOGY LA English DT Article DE carbon dioxide emission; phospholipid fatty acids; keeling plot; stable isotope; water level changes ID DISSOLVED ORGANIC-CARBON; FRESH-WATER; VERTICAL-DISTRIBUTION; MATTER; SOILS; DECOMPOSITION; CYCLE; MINERALIZATION; TERRESTRIAL; PHOSPHORUS AB Due to climate change, many lakes in Europe will be subject to higher variability of hydrological characteristics in their littoral zones. These different hydrological regimes might affect the use of allochthonous and autochthonous carbon sources. We used sandy sediment microcosms to examine the effects of different hydrological regimes (wet, desiccating, and wet-desiccation cycles) on carbon turnover. C-13-labelled particulate organic carbon was used to trace and estimate carbon uptake into bacterial biomass (via phospholipid fatty acids) and respiration. Microbial community changes were monitored by combining DNA-and RNA-based real-time PCR quantification and terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA. The shifting hydrological regimes in the sediment primarily caused two linked microbial effects: changes in the use of available organic carbon and community composition changes. Drying sediments yielded the highest CO2 emission rates, whereas hydrological shifts increased the uptake of allochthonous organic carbon for respiration. T-RFLP patterns demonstrated that only the most extreme hydrological changes induced a significant shift in the active and total bacterial communities. As current scenarios of climate change predict an increase of drought events, frequent variations of the hydrological regimes of many lake littoral zones in central Europe are anticipated. Based on the results of our study, this phenomenon may increase the intensity and amplitude in rates of allochthonous organic carbon uptake and CO2 emissions. C1 [Weise, Lukas; Ulrich, Andreas; Moreano, Matilde; Gessler, Arthur; Kayler, Zachary E.; Premke, Katrin] Leibniz Ctr Agr Landscape Res ZALF Muncheberg, Inst Landscape Biogeochem, Eberswalderstr 84, D-15374 Muncheberg, Germany. [Gessler, Arthur] Swiss Fed Res Inst WSL, Zurcherstr 111, CH-8903 Birmensdorf, Switzerland. [Gessler, Arthur] Berlin Brandenburg Inst Adv Biodivers Res BBIB, D-14195 Berlin, Germany. [Steger, Kristin] Univ Calif Davis, Dept Viticulture & Enol, One Shields Ave, Davis, CA 95616 USA. [Zeller, Bernd] INRA, Ctr Nancy Lorraine, Biogeochim Ecosyst Forestiers, Labex ARBRE,UR 1138, F-54280 Champenoux, France. [Rudolph, Kristin] TU Chemnitz, Dept Psychol Res Methods & Evaluat, D-09107 Chemnitz, Germany. [Knezevic-Jaric, Jelena; Premke, Katrin] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Chem Analyt & Biogeochem, Muggelseedamm 310, D-12587 Berlin, Germany. [Kayler, Zachary E.] Lawrence Livermore Natl Lab, US Forest Serv, USDA, No Res Stn, Livermore, CA 94550 USA. RP Premke, K (reprint author), Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Chem Analyt & Biogeochem, Muggelseedamm 310, D-12587 Berlin, Germany. EM premke@igb-berlin.de RI Gessler, Arthur/C-7121-2008; Ulrich, Andreas/G-3956-2012 OI Gessler, Arthur/0000-0002-1910-9589; Ulrich, Andreas/0000-0003-3854-9608 FU LandScales project ('Connecting processes and structures driving the landscape carbon dynamics over scales') - Leibniz Association within the Joint Initiative for Research and Innovation FX Financial support was provided by the LandScales project ('Connecting processes and structures driving the landscape carbon dynamics over scales') financed by the Leibniz Association within the Joint Initiative for Research and Innovation. NR 74 TC 5 Z9 5 U1 14 U2 31 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0168-6496 EI 1574-6941 J9 FEMS MICROBIOL ECOL JI FEMS Microbiol. Ecol. PD MAY PY 2016 VL 92 IS 5 AR fiw035 DI 10.1093/femsec/fiw035 PG 14 WC Microbiology SC Microbiology GA DO0MM UT WOS:000377473000003 PM 26902802 ER PT J AU Sun, DY Nandety, RS Zhang, YL Reid, MS Niu, LX Jiang, CZ AF Sun, Daoyang Nandety, Raja Sekhar Zhang, Yanlong Reid, Michael S. Niu, Lixin Jiang, Cai-Zhong TI A petunia ethylene-responsive element binding factor, PhERF2, plays an important role in antiviral RNA silencing SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Argonaute; cucumber; mosaic; virus; dicer-like enzyme; RNA-dependent RNA polymerase; tobacco; rattle; virus; transcription factor; virus-induced gene silencing ID TOBACCO-RATTLE-VIRUS; SMALL INTERFERING RNAS; DICER-LIKE PROTEINS; TRANSCRIPTION FACTOR; ARABIDOPSIS-THALIANA; FLOWER SENESCENCE; LOW-TEMPERATURE; NICOTIANA-BENTHAMIANA; SALT TOLERANCE; PLANT AB PhERF2, an ethylene-responsive element binding factor, acts as a positive transcriptional regulator in antiviral RNA silencing and is essential for efficient silencing of genes in plants.Virus-induced RNA silencing is involved in plant antiviral defense and requires key enzyme components, including RNA-dependent RNA polymerases (RDRs), Dicer-like RNase III enzymes (DCLs), and Argonaute proteins (AGOs). However, the transcriptional regulation of these critical components is largely unknown. In petunia (Petunia hybrida), an ethylene-responsive element binding factor, PhERF2, is induced by Tobacco rattle virus (TRV) infection. Inclusion of a PhERF2 fragment in a TRV silencing construct containing reporter fragments of phytoene desaturase (PDS) or chalcone synthase (CHS) substantially impaired silencing efficiency of both the PDS and CHS reporters. Silencing was also impaired in PhERF2- RNAi lines, where TRV-PhPDS infection did not show the expected silencing phenotype (photobleaching). In contrast, photobleaching in response to infiltration with the TRV-PhPDS construct was enhanced in plants overexpressing PhERF2. Transcript abundance of the RNA silencing-related genes RDR2, RDR6, DCL2, and AGO2 was lower in PhERF2-silenced plants but higher in PhERF2-overexpressing plants. Moreover, PhERF2-silenced lines showed higher susceptibility to Cucumber mosaic virus (CMV) than wild-type (WT) plants, while plants overexpressing PhERF2 exhibited increased resistance. Interestingly, growth and development of PhERF2-RNAi lines were substantially slower, whereas the overexpressing lines were more vigorous than the controls. Taken together, our results indicate that PhERF2 functions as a positive regulator in antiviral RNA silencing. C1 [Sun, Daoyang; Zhang, Yanlong; Niu, Lixin] Northwest A&F Univ, Dept Landscape Architecture & Arts, Yangling 712100, Shaanxi, Peoples R China. [Sun, Daoyang; Reid, Michael S.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Nandety, Raja Sekhar] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Jiang, Cai-Zhong] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. RP Niu, LX (reprint author), Northwest A&F Univ, Dept Landscape Architecture & Arts, Yangling 712100, Shaanxi, Peoples R China.; Jiang, CZ (reprint author), ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. EM niulixin@nwsuaf.edu.cn; cjiang@ucdavis.edu OI Jiang, Cai-Zhong/0000-0002-5972-7963 FU United States Department of Agriculture (USDA) CRIS project [5306-21000-019-00D] FX We thank Dr Bryce Falk for technical assistance in the northern blot analysis and Xiaoqing Xie for help in the transformation experiments. Dr Weixing Shan kindly hosted some of the experiments in his laboratory. We appreciate Lee Ann Richmond's assistance with the analytical instrumentation. This work was partially supported by United States Department of Agriculture (USDA) CRIS project 5306-21000-019-00D. NR 60 TC 3 Z9 3 U1 6 U2 16 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAY PY 2016 VL 67 IS 11 BP 3353 EP 3365 DI 10.1093/jxb/erw155 PG 13 WC Plant Sciences SC Plant Sciences GA DO0LZ UT WOS:000377471700017 PM 27099376 ER PT J AU Marsh, AP Applegate, WB Guralnik, JM Rejeski, WJ Church, TS Fielding, RA Gill, TM King, AC Kritchevsky, SB Manini, TM McDermott, MM Newman, AB Stowe, CL Walkup, MP Pahor, M Miller, ME AF Marsh, Anthony P. Applegate, William B. Guralnik, Jack M. Rejeski, W. Jack Church, Timothy S. Fielding, Roger A. Gill, Thomas M. King, Abby C. Kritchevsky, Stephen B. Manini, Todd M. McDermott, Mary M. Newman, Anne B. Stowe, Cynthia L. Walkup, Michael P. Pahor, Marco Miller, Michael E. CA LIFE Study Investigators TI Hospitalizations During a Physical Activity Intervention in Older Adults at Risk of Mobility Disability: Analyses from the Lifestyle Interventions and Independence for Elders Randomized Clinical Trial SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article DE physical activity; older adults; mobility disability; safety; hospitalizations ID GAIT SPEED; HEALTH; PERFORMANCE; ASSOCIATION; MORTALITY; WALKING; COSTS AB ObjectivesTo determine whether moderate-intensity physical activity (PA) and health education (HE) are differentially associated with categories of hospitalizations or subgroups of participants. DesignMulticenter randomized controlled trial in which participants were randomized to a PA or HE program for an average of 2.6years. SettingEight field centers. ParticipantsSedentary men and women aged 70-89 with lower extremity physical limitations but able to walk 400-m in 15minutes or less (N=1,635). InterventionsStructured, moderate-intensity PA (n=818) at a center (2x/wk) and at home (3-4x/wk) that included aerobic, strength, balance, and flexibility training or HE (n=817) of educational workshops and upper extremity stretching exercises. MeasurementsAll-cause inpatient hospitalizations ascertained at 6-month intervals. ResultsThere were 1,458 hospitalizations (49.1% of PA, 44.4% of HE; risk difference=4.68%, 95% confidence interval (CI)=-0.18-9.54; hazard ratio (HR)=1.16, 95% CI=1.00-1.34). The intervention effect on incident hospitalization did not differ according to race, sex, Short Physical Performance Battery score, age, or history of cardiovascular disease or diabetes mellitus. PA was associated with higher rates of hospitalization in the middle baseline gait speed category, than HE (<0.8m/s: HR=0.93, 95% CI=0.76-1.14; 0.8-1.0m/s: HR=1.54, 95% CI=1.23-1.94; >1.0m/s: HR=1.05, 95% CI=0.67-1.65; interaction P=.005). ConclusionA PA program in older adults at risk for mobility disability did not lead to a different risk of specific types of hospitalizations than a HE program overall. Baseline gait speed may be a marker for risk of hospitalization during a PA intervention, because individuals with moderate baseline gait speed in the PA group had slightly higher rates of hospitalization than those in the HE group. Trial Registration: ClinicalTrials.gov identifier: NCT01072500. C1 [Marsh, Anthony P.; Rejeski, W. Jack] Wake Forest Univ, Dept Hlth & Exercise Sci, POB 7868, Winston Salem, NC 27109 USA. [Applegate, William B.; Kritchevsky, Stephen B.] Wake Forest Univ, Dept Internal Med, Sticht Ctr Aging, Sect Gerontol & Geriatr Med, Winston Salem, NC 27109 USA. [Stowe, Cynthia L.; Walkup, Michael P.; Miller, Michael E.] Wake Forest Univ, Sch Med, Dept Biostat Sci, Div Publ Hlth Sci, Winston Salem, NC 27109 USA. [Guralnik, Jack M.; Church, Timothy S.] Univ Maryland, Sch Med, Dept Epidemiol & Publ Hlth, Div Gerontol, Baltimore, MD 21201 USA. [Fielding, Roger A.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Gill, Thomas M.] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA. [King, Abby C.] Stanford Univ, Dept Hlth Res & Policy, Sch Med, Stanford, CA 94305 USA. [King, Abby C.] Stanford Univ, Dept Med, Sch Med, Stanford, CA 94305 USA. [Manini, Todd M.; McDermott, Mary M.; Pahor, Marco] Univ Florida, Coll Med, Inst Aging, Dept Aging & Geriatr Res, Gainesville, FL USA. [Manini, Todd M.; McDermott, Mary M.; Pahor, Marco] Northwestern Univ, Feinberg Sch Med, Dept Med & Prevent Med, Chicago, IL 60611 USA. [Newman, Anne B.] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Epidemiol & Med, Pittsburgh, PA USA. RP Marsh, AP (reprint author), Wake Forest Univ, Dept Hlth & Exercise Sci, POB 7868, Winston Salem, NC 27109 USA. EM marshap@wfu.edu RI Buford, Thomas /B-1111-2011 OI Buford, Thomas /0000-0002-9541-4358 FU National Institutes of Health (NIH); U.S. Department of Agriculture; Nestle; Regeneron; NIH FX All authors were supported by a National Institutes of Health (NIH) grant to work on this study. Roger A. Fielding has received grants or funds from the NIH, U.S. Department of Agriculture, and Nestle, Regeneron and honoraria from Nestle and Regeneron; is a consultant for Eli Lilly, Regereron, Nestle; owns stock in Pronutria, Myosyntax, and Inside Trader; and is a board member for Cytokinetics, Pronutria, Myosyntax, Inside Trader, and Ammonett.; The NIH sponsor was a voting member (1 of 12 votes) of the LIFE Steering Committee, which approved the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, and approval of the manuscript; and decision to submit the manuscript for publication. NR 24 TC 2 Z9 2 U1 5 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0002-8614 EI 1532-5415 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD MAY PY 2016 VL 64 IS 5 BP 933 EP 943 DI 10.1111/jgs.14114 PG 11 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DO1FD UT WOS:000377523000001 PM 27225353 ER PT J AU Sutton, EF Gilmore, LA Dunger, DB Heijmans, BT Hivert, MF Ling, C Martinez, JA Ozanne, SE Simmons, RA Szyf, M Waterland, RA Redman, LM Ravussin, E AF Sutton, Elizabeth F. Gilmore, L. Anne Dunger, David B. Heijmans, Bas T. Hivert, Marie-France Ling, Charlotte Martinez, J. Alfredo Ozanne, Susan E. Simmons, Rebecca A. Szyf, Moshe Waterland, Robert A. Redman, Leanne M. Ravussin, Eric TI Developmental Programming: State-of-the-Science and Future Directions-Summary from a Pennington Biomedical Symposium SO OBESITY LA English DT Review ID CORONARY-HEART-DISEASE; ADULT BODY-COMPOSITION; LONG NONCODING RNAS; CATCH-UP GROWTH; PROTEIN-RESTRICTED DIET; POOR LIVING-CONDITIONS; DNA METHYLATION; BIRTH-WEIGHT; LATER LIFE; EPIGENETIC REGULATION AB Objective: On December 8-9, 2014, the Pennington Biomedical Research Center convened a scientific symposium to review the state-of-the-science and future directions for the study of developmental programming of obesity and chronic disease. The objectives of the symposium were to discuss: (i) past and current scientific advances in animal models, population-based cohort studies, and human clinical trials, (ii) the state-of-the-science of epigenetic-based research, and (iii) considerations for future studies. Results: This symposium provided a comprehensive assessment of the state of the scientific field and identified research gaps and opportunities for future research in order to understand the mechanisms contributing to the developmental programming of health and disease. Conclusions: Identifying the mechanisms which cause or contribute to developmental programming of future generations will be invaluable to the scientific and medical community. The ability to intervene during critical periods of prenatal and early postnatal life to promote lifelong health is the ultimate goal. Considerations for future research including the use of animal models, the study design in human cohorts with considerations about the timing of the intrauterine exposure, and the resulting tissue-specific epigenetic signature were extensively discussed and are presented in this meeting summary. C1 [Sutton, Elizabeth F.; Gilmore, L. Anne; Redman, Leanne M.; Ravussin, Eric] Pennington Biomed Res Ctr, Clin Sci, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. [Dunger, David B.] Univ Cambridge, Dept Paediat, Cambridge, England. [Heijmans, Bas T.] Leiden Univ, Med Ctr, The Hague Area, NL-2300 RA Leiden, Netherlands. [Hivert, Marie-France] Harvard Univ, Sch Med, Dept Populat Med, Boston, MA USA. [Ling, Charlotte] Lund Univ, Dept Clin Sci, Malmo, Sweden. [Martinez, J. Alfredo] Univ Navarra, Dept Human Nutr, CIBERobn, Navarra, Spain. [Martinez, J. Alfredo] IDISNA, Navarra, Spain. [Ozanne, Susan E.] Univ Cambridge, Dept Clin Biochem, Cambridge, England. [Simmons, Rebecca A.] Perelman Sch Med, Dept Pediat, Philadelphia, PA USA. [Szyf, Moshe] McGill Univ, Dept Pharmacol, Montreal, PQ H3A 2T5, Canada. [Waterland, Robert A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat Nutr,Baylor Coll Med, Houston, TX 77030 USA. RP Ravussin, E (reprint author), Pennington Biomed Res Ctr, Clin Sci, 6400 Perkins Rd, Baton Rouge, LA 70808 USA. EM eric.ravussin@pbrc.edu RI Ling, Charlotte/Q-2432-2015; OI Ling, Charlotte/0000-0003-0587-7154; Ozanne, Susan/0000-0001-8753-5144 FU NORC Center Grant from the NIDDK [P30DK072476]; NIDDK [T32DK064584]; Canadian Institute of Health Research [MOP-42411]; Center for Nutrition Research at the University of Navarra in Pamplona, Spain; U.S. Department of Agriculture (USDA) [CRIS 3092-5-001-059]; National Institutes of Health [R01AG042190]; European Union [259679]; Swedish Research Council; Novo Nordisk Foundation; American Diabetes Association (ADA) Pathways To Stop Diabetes Award; NORC [P30DK072476] FX The symposium was funded by NORC Center Grant P30DK072476 from the NIDDK. LAG is supported by T32DK064584 from the NIDDK. Work in MS Laboratory was supported by MOP-42411 from the Canadian Institute of Health Research. Work in JAM Laboratory was supported by the Center for Nutrition Research at the University of Navarra in Pamplona, Spain. RAW is supported by a grant from the U.S. Department of Agriculture (USDA) [CRIS 3092-5-001-059]. Work in BTH Laboratory was supported by the National Institutes of Health (R01AG042190) and the European Union's Seventh Framework Program IDEAL (FP7/2007-2011; grant agreement No. 259679). Work in CL Laboratory was funded by The Swedish Research Council and The Novo Nordisk Foundation. SEO is a member of the University of Cambridge MRC Metabolic Diseases Unit. MFH is the recipient of an American Diabetes Association (ADA) Pathways To Stop Diabetes Award. Work in ER and LMR Laboratories was partially funded by a NORC grant titled "Nutritional Programming: Environmental and Molecular Interactions" to ER (P30DK072476). NR 95 TC 0 Z9 0 U1 2 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1930-7381 EI 1930-739X J9 OBESITY JI Obesity PD MAY PY 2016 VL 24 IS 5 BP 1018 EP 1026 DI 10.1002/oby.21487 PG 9 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA DO1DU UT WOS:000377519500006 PM 27037645 ER PT J AU Rasooly, R Do, P Hernlem, B AF Rasooly, Reuven Do, Paula Hernlem, Bradley TI Sensitive, Rapid, Quantitative and in Vitro Method for the Detection of Biologically Active Staphylococcal Enterotoxin Type E SO TOXINS LA English DT Article DE styphlococcal enterotoxin type E; T-cells; splenocyte ID TOXIC-SHOCK-SYNDROME; INTRAVENOUS IMMUNOGLOBULIN THERAPY; T-CELLS; GENE-EXPRESSION; FOOD; AUREUS; ASSAY; SUPERANTIGENS; QUANTIFICATION; IDENTIFICATION AB Staphylococcus aureus is a major bacterial cause of clinical infections and foodborne illnesses through its production of a group of enterotoxins (SEs) which cause gastroenteritis and also function as superantigens to massively activate T cells. In the present study, we tested Staphylococcal enterotoxin type E (SEE), which was detected in 17 of the 38 suspected staphylococcal food poisoning incidents in a British study and was the causative agent in outbreaks in France, UK and USA. The current method for detection of enterotoxin activity is an in vivo monkey or kitten bioassay; however, this expensive procedure has low sensitivity and poor reproducibility, requires many animals, is impractical to test on a large number of samples, and raises ethical concerns with regard to the use of experimental animals. The purpose of this study is to develop rapid sensitive and quantitative bioassays for detection of active SEE. We apply a genetically engineered T cell-line expressing the luciferase reporter gene under the regulation of nuclear factor of activated T-cells response element (NFAT-RE), combined with a Raji B-cell line that presents the SEE-MHC (major histocompatibility complex) class II to the engineered T cell line. Exposure of the above mixed culture to SEE induces differential expression of the luciferase gene and bioluminescence is read out in a dose dependent manner over a 6-log range. The limit of detection of biologically active SEE is 1 fg/mL which is 10(9) times more sensitive than the monkey and kitten bioassay. C1 [Rasooly, Reuven; Do, Paula; Hernlem, Bradley] USDA ARS, Western Reg Res Ctr, Foodborne Toxin Detect & Prevent Res Unit, 800 Buchanan St, Albany, CA 94710 USA. RP Rasooly, R (reprint author), USDA ARS, Western Reg Res Ctr, Foodborne Toxin Detect & Prevent Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov; paula.do@ars.usda.gov; bradley.hernlem@ars.usda.gov NR 35 TC 0 Z9 0 U1 4 U2 4 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD MAY PY 2016 VL 8 IS 5 AR 150 DI 10.3390/toxins8050150 PG 11 WC Toxicology SC Toxicology GA DN9VH UT WOS:000377428300031 ER PT J AU Wilson, WC Davis, AS Gaudreault, NN Faburay, B Trujillo, JD Shivanna, V Sunwoo, SY Balogh, A Endalew, A Ma, W Drolet, BS Ruder, MG Morozov, I McVey, DS Richt, JA AF Wilson, William C. Davis, A. Sally Gaudreault, Natasha N. Faburay, Bonto Trujillo, Jessie D. Shivanna, Vinay Sunwoo, Sun Young Balogh, Aaron Endalew, Abaineh Ma, Wenjun Drolet, Barbara S. Ruder, Mark G. Morozov, Igor McVey, D. Scott Richt, Juergen A. TI Experimental Infection of Calves by Two Genetically-Distinct Strains of Rift Valley Fever Virus SO VIRUSES-BASEL LA English DT Article DE Challenge model; Rift Valley fever; Rift Valley fever virus; Cattle; pathogenicity ID NORTH-AMERICAN MOSQUITOS; VACCINE DEVELOPMENT; CHALLENGE MODEL; EUROPEAN-UNION; SAUDI-ARABIA; SHEEP; IMMUNOGENICITY; SAFETY; EFFICACY; MP-12 AB Recent outbreaks of Rift Valley fever in ruminant livestock, characterized by mass abortion and high mortality rates in neonates, have raised international interest in improving vaccine control strategies. Previously, we developed a reliable challenge model for sheep that improves the evaluation of existing and novel vaccines in sheep. This sheep model demonstrated differences in the pathogenesis of Rift Valley fever virus (RVFV) infection between two genetically-distinct wild-type strains of the virus, Saudi Arabia 2001 (SA01) and Kenya 2006 (Ken06). Here, we evaluated the pathogenicity of these two RVFV strains in mixed breed beef calves. There was a transient increase in rectal temperatures with both virus strains, but this clinical sign was less consistent than previously reported with sheep. Three of the five Ken06-infected animals had an early-onset viremia, one day post-infection (dpi), with viremia lasting at least three days. The same number of SA01-infected animals developed viremia at 2 dpi, but it only persisted through 3 dpi in one animal. The average virus titer for the SA01-infected calves was 1.6 logs less than for the Ken06-infected calves. Calves, inoculated with either strain, seroconverted by 5 dpi and showed time-dependent increases in their virus-neutralizing antibody titers. Consistent with the results obtained in the previous sheep study, elevated liver enzyme levels, more severe liver pathology and higher virus titers occurred with the Ken06 strain as compared to the SA01 strain. These results demonstrate the establishment of a virulent challenge model for vaccine evaluation in calves. C1 [Wilson, William C.; Gaudreault, Natasha N.; Drolet, Barbara S.; Ruder, Mark G.; McVey, D. Scott] ARS, USDA, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. [Davis, A. Sally; Faburay, Bonto; Trujillo, Jessie D.; Shivanna, Vinay; Sunwoo, Sun Young; Balogh, Aaron; Endalew, Abaineh; Ma, Wenjun; Morozov, Igor; Richt, Juergen A.] Kansas State Univ, Dept Diagnost Med Pathobiol, Coll Vet Med, Manhattan, KS 66502 USA. [Gaudreault, Natasha N.] Kansas State Univ, Dept Diagnost Med Pathobiol, Coll Vet Med, Manhattan, KS 66502 USA. [Ruder, Mark G.] Univ Georgia, Coll Vet Med, Southeastern Cooperat Wildlife Dis Study, Athens, GA 30602 USA. RP Wilson, WC (reprint author), ARS, USDA, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA.; Richt, JA (reprint author), Kansas State Univ, Dept Diagnost Med Pathobiol, Coll Vet Med, Manhattan, KS 66502 USA. EM William.Wilson@ars.usda.gov; asally@vet.k-state.edu; nng5757@vet.k-state.edu; bfaburay@vet.k-state.edu; jdtrujillo@vet.k-state.edu; vinays@vet.k-state.edu; sunwoo@ksu.edu; balogh@vet.k-state.edu; adendale@vet.k-state.edu; wma@vet.k-state.edu; Barbara.Drolet@ars.usda.gov; mgruder@uga.edu; imorozov@vet.k-state.edu; Scott.McVey@ars.usda.gov; jricht@ksu.edu OI Shivanna, Vinay/0000-0002-4625-954X; Wilson, William/0000-0002-7877-9224 FU Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) [2010-ST061-AG0001]; Kansas Bioscience Authority; National Bio and Agro-Defense Facility (NBAF) Transition Funds; USDA Agricultural Services Project [3020-32000-005-00D] FX The authors wish to thank the staff of KSU Biosecurity Research Institute, the Comparative Medicine Group and the staff of the Histopathology Unit at the Veterinary Diagnostic Laboratory. We wish to thank Dane Jasperson, Lindsey Reister-Hendricks (Arthropod-Borne Animal Diseases Research Unit, USDA/Agricultural Research Service) and Chester McDowell, Tammy Koopman, Haixia Liu, Maira Cotton-Caballero, Elizabeth Stietzle, Monica Gamez and Mal Hoover (KSU) for technical assistance. We also thank Mal Rooks Hoover for assistance in medical illustration. This work was funded by the grants of the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Grant No. 2010-ST061-AG0001, the Kansas Bioscience Authority, the National Bio and Agro-Defense Facility (NBAF) Transition Funds and USDA Agricultural Services Project 3020-32000-005-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 44 TC 0 Z9 0 U1 2 U2 2 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4915 J9 VIRUSES-BASEL JI Viruses-Basel PD MAY PY 2016 VL 8 IS 5 AR 145 DI 10.3390/v8050145 PG 16 WC Virology SC Virology GA DN9VU UT WOS:000377429600028 ER PT J AU Ramsay, TG Blomberg, L Caperna, TJ AF Ramsay, T. G. Blomberg, L. Caperna, T. J. TI alpha 1-acid glycoprotein inhibits lipogenesis in neonatal swine adipose tissue SO ANIMAL LA English DT Article DE lipogenesis; alpha 1-acid glycoprotein; neonate; swine; adipose ID ELEMENT-BINDING PROTEIN-1C; MAJOR PLASMA-PROTEINS; ACUTE-PHASE PROTEINS; GENE-EXPRESSION; ALPHA(1)-ACID GLYCOPROTEIN; NUTRITIONAL REGULATION; SREBP-1C PROMOTER; LIPID-METABOLISM; HEALTH-STATUS; SERUM AB Serum alpha 1-acid glycoprotein (AGP) is elevated during late gestation and at birth in the pig and rapidly declines postnatally. In contrast, the pig is born with minimal lipid stores in the adipose tissue, but rapidly accumulates lipid during the first week. The present study examined if AGP can affect adipose tissue metabolism in the neonatal pig. Isolated cell cultures or tissue explants were prepared from dorsal subcutaneous adipose tissue of preweaning piglets. Porcine AGP was used at concentrations of 0, 100, 1000 and 5000 ng/ml medium in 24 h incubations. AGP reduced the messenger RNA (mRNA) abundance of the lipogenic enzymes, malic enzyme (ME), fatty acid synthase and acetyl coA carboxylase by at least 40% (P < 0.001). The activity of ME and citrate lyase were also reduced by AGP (P < 0.05). Glucose oxidation was reduced by treatment with 5000 ng AGP/ml medium (P < 0.05). The C-14-glucose incorporation into fatty acids was reduced by similar to 25% by AGP treatment for 24 h with 1000 ng AGP/ml medium (P < 0.05). The decrease in glucose metabolism by AGP appears to function through an inhibition in insulin-mediated glucose oxidation and incorporation into fatty acids. This was supported by the analysis of the mRNA abundance for sterol regulatory element-binding protein (SREBP), carbohydrate regulatory element-binding protein (ChREBP) and insulin receptor substrate 1 (IRS1), which all demonstrated reductions of at least 23% in response to AGP treatment (P < 0.05). These data demonstrate an overall suppression of lipogenesis due to AGP inhibition of lipogenic gene expression in vitro, which the metabolic data and SREBP, ChREBP and IRS1 gene expression analysis suggest is through an inhibition in insulin-mediated events. Second, these data suggest that AGP may contribute to limiting lipogenesis within adipose tissue during the perinatal period, as AGP levels are highest for any serum protein at birth. C1 [Ramsay, T. G.; Blomberg, L.; Caperna, T. J.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Anim & Nat Resources Inst,USDA, Beltsville, MD 20705 USA. RP Ramsay, TG (reprint author), ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Anim & Nat Resources Inst,USDA, Beltsville, MD 20705 USA. EM timothy.ramsay@ars.usda.gov NR 46 TC 1 Z9 1 U1 2 U2 3 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD MAY PY 2016 VL 10 IS 5 BP 812 EP 820 DI 10.1017/S1751731115002414 PG 9 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA DN5QQ UT WOS:000377125600010 PM 26608612 ER PT J AU Luppold, WG Bumgardner, MS AF Luppold, William G. Bumgardner, Matthew S. TI Employment Changes in US Hardwood Lumber Consuming Industries during Economic Expansions and Contractions Since 1991 SO BIORESOURCES LA English DT Article DE Employment; Hardwood lumber processors; Kitchen cabinets; Millwork; Pallets; Secondary hardwood industry; Wood household furniture ID PRODUCTS; IMPACTS AB Understanding employment trends is important for discerning the economic vitality of U.S. hardwood lumber users. After a period of growth in the 1990s, employment in industries consuming hardwood lumber has declined in the 21st century. The wood household furniture industry has experienced the greatest decline, with North Carolina, Virginia, and California being the states most affected. Nearly all of the decline in employment in the furniture industry can be attributed to increased importation of this product. Millwork and kitchen cabinets are industries associated with home construction. Both of these industries experienced declines in employment in the 21st century. Employment in millwork started to decline after 2000, while employment in the kitchen cabinet industry started to decline after 2006. While there was little change in the relative regional employment rankings in the millwork industry, Indiana displaced California and Texas to become the largest employer in the kitchen cabinet industry. Employment in the pallet industry has declined in the 21st century, but mostly during the two recessionary periods. The pallet industry was the only industry that had an employment increase after 2009. Ohio was once the major pallet producing state, but it was displaced by California and Texas after 2002. C1 [Luppold, William G.] US Forest Serv, No Res Stn, 241 Mercer Springs Rd, Princeton, WV 24740 USA. [Bumgardner, Matthew S.] US Forest Serv, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. RP Luppold, WG (reprint author), US Forest Serv, No Res Stn, 241 Mercer Springs Rd, Princeton, WV 24740 USA. EM wluppold@fs.fed.us NR 19 TC 0 Z9 0 U1 1 U2 1 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD MAY PY 2016 VL 11 IS 2 BP 3123 EP 3135 PG 13 WC Materials Science, Paper & Wood SC Materials Science GA DL6YT UT WOS:000375786700014 ER PT J AU Llana, DF Iniguez-Gonzalez, G Arriaga, F Wang, XP AF Llana, Daniel F. Iniguez-Gonzalez, Guillermo Arriaga, Francisco Wang, Xiping TI Time-of-Flight Adjustment Procedure for Acoustic Measurements in Structural Timber SO BIORESOURCES LA English DT Article DE Length effect; Sawn timber; Time-of-flight; Ultrasonic wave; Vibration analysis; Wave velocity ID WOOD; ULTRASOUND; VELOCITY AB The effect of timber length on time-of-flight acoustic longitudinal measurements was investigated on the structural timber of four Spanish species: radiata pine (Pinus radiata D. Don), Scots pine (Pinus sylvestris L.), laricio pine (Pinus nigra Arn.), and maritime pine (Pinus pinaster Ait.). Time-of-flight longitudinal measurements were conducted on 120 specimens of dimensions 90 x 140 mm using three commercially available acoustic instruments (Sylvatest Duo, USLab, and Microsecond Timer). Time-of-flight data were initially obtained from the full-length (4 m) specimens, and then from the specimens cut to 3, 2, and 1 meter in length by successively cutting off 0.5 m from each end. The acoustic longitudinal velocity of the timber specimens of different lengths was also measured using a resonance-based acoustic method. The apparent acoustic longitudinal velocity for all species increased linearly as the timber length decreased from 4 to 1 meter. Acoustic velocity determined from time-of-flight data was significantly higher than the acoustic velocity determined using the resonance method, indicating systematic measurement errors associated with the time-of-flight instruments. Empirical models were developed for the relationships between time-off-light measurements on timber specimens and timber lengths in the range of 1 to 4 m. Finally, a procedure was proposed to correct the time-of-flight data. C1 [Llana, Daniel F.; Iniguez-Gonzalez, Guillermo; Arriaga, Francisco] Univ Politecn Madrid, ETSI Montes Forestal & Medio Nat, E-28040 Madrid, Spain. [Wang, Xiping] US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53705 USA. RP Llana, DF (reprint author), Univ Politecn Madrid, ETSI Montes Forestal & Medio Nat, E-28040 Madrid, Spain. EM danielfllana@gmail.com RI Arriaga, Francisco/L-3330-2014; OI Arriaga, Francisco/0000-0001-5535-0786; F. Llana, Daniel/0000-0001-7758-9456 FU Ministerio de Economia y Competitividad, Espana (Ministry of Economy and Competitiveness, Spain, State Plan, Research and Development) [BIA2014-55089-P, BIA 2010-18858] FX The authors acknowledge the Ministerio de Economia y Competitividad, Espana, Programa Estatal I+D, 2013-2016 (Ministry of Economy and Competitiveness, Spain, State Plan, Research and Development), Proy.: BIA2014-55089-P, Plan Nacional I+D+i 2008-2011, Proy.: BIA 2010-18858 for financial support to Mr. Daniel F. Llana's visiting program at the U.S. Forest Service Forest Products Laboratory, and Mr. Ramon Garcia Lombardero of Timber Structural Laboratory of CIFOR-INIA, Spain, for his technical assistance in this study. NR 24 TC 0 Z9 0 U1 1 U2 1 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD MAY PY 2016 VL 11 IS 2 BP 3303 EP 3317 PG 15 WC Materials Science, Paper & Wood SC Materials Science GA DL6YT UT WOS:000375786700027 ER PT J AU Guan, C Zhang, HJ Hunt, JF Zhou, LJ Feng, D AF Guan, Cheng Zhang, Houjiang Hunt, John F. Zhou, Lujing Feng, Dan TI Measurement of Dynamic Viscoelasticity of Full-Size Wood Composite Panels Using a Vibration Testing Method SO BIORESOURCES LA English DT Article DE Full-size wood composite panel; Dynamic viscoelasticity; Storage modulus; Loss modulus; Vibration testing ID MEDIUM-DENSITY FIBERBOARD; ELASTICITY; MODULUS AB The dynamic viscoelasticity of full-size wood composite panels (WCPs) under the free-free vibrational state were determined by a vibration testing method. Vibration detection tests were performed on 194 pieces of three types of full-size WCPs (particleboard, medium density fiberboard, and plywood (PW)). The dynamic viscoelasticity from smaller specimens cut from the panels was measured using a cantilever beam vibration test apparatus, and the two data sets were compared. A strong linear relationship was discovered between the dynamic viscoelasticity values measured by the vibration detection test and the cantilever beam vibration test. The storage modulus values of the panels were far higher than their loss modulus values, and PW panels had the smallest value of loss modulus. For the panels tested, density had a good linear impact on storage modulus. In comparison with density, logarithmic decrement had a greater linear impact on loss modulus. This study demonstrated that the vibration test method is a valid approach for determining the dynamic viscoelasticity of full-size WCPs. C1 [Guan, Cheng; Zhang, Houjiang; Zhou, Lujing; Feng, Dan] Beijing Forestry Univ, Sch Technol, Beijing 100083, Peoples R China. [Hunt, John F.] USDA Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. RP Zhang, HJ (reprint author), Beijing Forestry Univ, Sch Technol, Beijing 100083, Peoples R China. EM hjzhang6@bjfu.edu.cn FU State Forest Administration of China through the Special Research Funds for Public Welfare [201304512] FX This project was funded by the State Forest Administration of China through the Special Research Funds for Public Welfare (Grant No. 201304512). NR 20 TC 0 Z9 0 U1 2 U2 3 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD MAY PY 2016 VL 11 IS 2 BP 4593 EP 4604 PG 12 WC Materials Science, Paper & Wood SC Materials Science GA DL6YT UT WOS:000375786700119 ER PT J AU Fleming, MB Decker, SR Bedinger, PA AF Fleming, Margaret B. Decker, Stephen R. Bedinger, Patricia A. TI Investigating the Role of Extensin Proteins in Poplar Biomass Recalcitrance SO BIORESOURCES LA English DT Article DE Extensin; Hydroxyproline-rich glycoprotein; Cellulosic biofuel; Biomass recalcitrance; Acid fungal protease; Poplar; Pretreatment ID PLANT-CELL-WALL; HYDROXYPROLINE-RICH GLYCOPROTEIN; SUSPENSION-CULTURES; LIGNOCELLULOSIC BIOMASS; CARROT EXTENSIN; GENE-EXPRESSION; CROSS-LINKING; AMINO-ACID; PRETREATMENT; ARABIDOPSIS AB The biological conversion of cellulosic biomass to biofuel is hindered by cell wall recalcitrance, which can limit the ability of cellulases to access and break down cellulose. The purpose of this study was to investigate whether hydroxyproline-rich cell wall proteins (extensins) are present in poplar stem biomass, and whether these proteins may contribute to recalcitrance. Three classical extensin genes were identified in Populus trichocarpa through bioinformatic analysis of poplar genome sequences, with the following proposed names: PtEXTENSIN1 (Potri. 001G019700); PtEXTENSIN2 (Potri. 001G020100); PtEXTENSIN3 (Potri. 018G050100). Tissue print immunoblots localized the extensin proteins in poplar stems to regions near the vascular cambium. Different thermochemical pretreatments reduced but did not eliminate hydroxyproline (Hyp, a proxy for extensins) from the biomass. Protease treatment of liquid hot water pretreated poplar biomass reduced Hyp content by a further 16% and increased subsequent glucose yield by 20%. These data suggest that extensins may contribute to recalcitrance in pretreated poplar biomass, and that incorporating protease treatment into pretreatment protocols could result in a small but significant increase in the yield of fermentable glucose. C1 [Fleming, Margaret B.; Bedinger, Patricia A.] Colorado State Univ, Dept Biol, Campus Delivery 1878, Ft Collins, CO 80523 USA. [Fleming, Margaret B.] USDA ARS, Natl Lab Genet Resources Preservat, 1111 South Mason St, Ft Collins, CO 80524 USA. [Decker, Stephen R.] Natl Renewable Energy Lab, Biosci Ctr, 15013 Denver West Pkwy, Golden, CO 80401 USA. [Decker, Stephen R.] Natl Renewable Energy Lab, BioEnergy Sci Ctr BESC, 15013 Denver West Pkwy, Golden, CO 80401 USA. RP Bedinger, PA (reprint author), Colorado State Univ, Dept Biol, Campus Delivery 1878, Ft Collins, CO 80523 USA. EM Pafricia.Bedinger@colostate.edu FU Colorado Center for Biorefining and Biofuels [013-7]; U.S. Department of Energy [DE-AC36-08GO28308]; National Renewable Energy Laboratory; DOE Office of Energy Efficiency and Renewable Energy, Bioenergy Technologies Office FX The authors thank the Colorado Center for Biorefining and Biofuels (Grant # 013-7), the Colorado State University Libraries Open Access Research and Scholarship Fund, CarboSource (Athens, GA) for JIM20, PlantProbes (Leeds, UK) for LM1, Dr. L. A. Staehelin (University of Colorado, Boulder) for gE-1, Dr. Suzanne Royer and Dr. Lorrie Anderson (CSU) for imaging assistance, Bronwyn Phillips and Olivia Todd for research assistance, Dr. Melvin P. Tucker (NREL) for providing the liquid hot water and dilute-acid pretreated poplar, and Dr. Emily Fox (University of Maryland) for a helpful and critical review of the manuscript.; The pretreatment and analytical work was partially supported by the U.S. Department of Energy under Contract No. DE-AC36-08GO28308 with the National Renewable Energy Laboratory. Funding was provided by the DOE Office of Energy Efficiency and Renewable Energy, Bioenergy Technologies Office. NR 75 TC 1 Z9 1 U1 3 U2 3 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD MAY PY 2016 VL 11 IS 2 BP 4727 EP 4744 PG 18 WC Materials Science, Paper & Wood SC Materials Science GA DL6YT UT WOS:000375786700128 ER PT J AU Rafique, N Tabassum, R Awan, MS Orts, W Wong, DWS AF Rafique, Nagina Tabassum, Romana Awan, Muhammad Siddique Orts, William Wong, Dominic W. S. TI Cloning and Expression of Pectobacterium carotovorum Endo-polygalacturonase Gene in Pichia pastoris for Production of Oligogalacturonates SO BIORESOURCES LA English DT Article DE Endo-polygalacturonase; Oligogalacturonate; Pectobacterium carotovorum; Pichia pastoris; Gene cloning ID SACCHAROMYCES-CEREVISIAE; GLYCEROL; PECTINS; ACIDS; CHROMATOGRAPHY AB A bacterial endo-polygalacturonase (endo-PGase) gene from the plant pathogen Pectobacterium carotovorum was cloned into pGAPZ alpha A vector and constitutively expressed in Pichia pastoris. The recombinant endo-PGase secreted by the Pichia clone showed a 1.7 fold increase when the culture medium included glycerol in replacement of glucose as the carbon source. The enzyme had optimum activity at pH 5.5 and 40 degrees C with stability between pH 5.0 and 8.0 and at temperatures up to 50 degrees C. The enzyme activity was enhanced by 41% with the addition of 1 mM Co++, and inhibited by Fe++ with a 63% reduction. The mode of the enzyme action showed internal cleavage of alpha-1,4 glycoside bonds of polygalacturonic acid and citrus peel pectin. Trigalacturonate and hexagalacturonate were the main hydrolysis products, with a yield of 0.44 +/- 0.01 and 0.21 +/- 0.01 mg released per mg polygalacturonic acid substrate, respectively. This represents the first report of a microbial endo-PGase that produced trimer and hexamer uniquely as the end products of hydrolysis, in contrast to mixtures of mono-, di-, and trigalacturonates commonly observed for the action of fungal enzymes. Pectic oligosaccharides generated from native carbohydrate polymers offer the potential application as building blocks for value-added products. C1 [Rafique, Nagina] Univ Azad Jammu & Kashmir, Fac Agr, Dept Food Technol, Muzaffarabad, Pakistan. [Tabassum, Romana] Natl Inst Biotechnol & Genet Engn, Faisalabad, Pakistan. [Awan, Muhammad Siddique] Univ Poonch, Fac Agr, Dept Food Technol, Wawalakot, Azad Kashmir, Pakistan. [Orts, William; Wong, Dominic W. S.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Wong, DWS (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM Dominic.Wong@ars.usda.gov FU International Research Support Initiative Program (IRSIP); Pakistan-U.S. Science and Technology Cooperation Program through USAID; Higher Education Commission of Pakistan FX The authors acknowledge support from the International Research Support Initiative Program (IRSIP) with the Higher Education Commission of Pakistan providing a travel stipend to Dr. Nagina Rafiq, as well as support from Pakistan-U.S. Science and Technology Cooperation Program funded through USAID. NR 26 TC 0 Z9 0 U1 5 U2 6 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD MAY PY 2016 VL 11 IS 2 BP 5204 EP 5214 PG 11 WC Materials Science, Paper & Wood SC Materials Science GA DL6YT UT WOS:000375786700162 ER PT J AU Eagles-Smith, CA Herring, G Johnson, B Graw, R AF Eagles-Smith, Collin A. Herring, Garth Johnson, Branden Graw, Rick TI Conifer density within lake catchments predicts fish mercury concentrations in remote subalpine lakes SO ENVIRONMENTAL POLLUTION LA English DT Article DE Mercury; Fish; Landscape; Bioaccumulation; Forest; Conifer; Condition factor ID FRESH-WATER ECOSYSTEMS; METHYL MERCURY; UNITED-STATES; ATMOSPHERIC DEPOSITION; YELLOW PERCH; SPORT FISH; CHEMISTRY; FOREST; METHYLMERCURY; CONTAMINATION AB Remote high-elevation lakes represent unique environments for evaluating the bioaccumulation of atmospherically deposited mercury through freshwater food webs, as well as for evaluating the relative importance of mercury loading versus landscape influences on mercury bioaccumulation. The increase in mercury deposition to these systems over the past century, coupled with their limited exposure to direct anthropogenic disturbance make them useful indicators for estimating how changes in mercury emissions may propagate to changes in Hg bioaccumulation and ecological risk. We evaluated mercury concentrations in resident fish from 28 high-elevation, sub-alpine lakes in the Pacific Northwest region of the United States. Fish total mercury (THg) concentrations ranged from 4 to 438 ng/g wet weight, with a geometric mean concentration (+/- standard error) of 43 +/- 2 ng/g ww. Fish THg concentrations were negatively correlated with relative condition factor, indicating that faster growing fish that are in better condition have lower THg concentrations. Across the 28 study lakes, mean THg concentrations of resident salmonid fishes varied as much as 18-fold among lakes. We used a hierarchal statistical approach to evaluate the relative importance of physiological, limnological, and catchment drivers of fish Hg concentrations. Our top statistical model explained 87% of the variability in fish THg concentrations among lakes with four key landscape and limnological variables: catchment conifer density (basal area of conifers within a lake's catchment), lake surface area, aqueous dissolved sulfate, and dissolved organic carbon. Conifer density within a lake's catchment was the most important variable explaining fish THg concentrations across lakes, with THg concentrations differing by more than 400 percent across the forest density spectrum. These results illustrate the importance of landscape characteristics in controlling mercury bioaccumulation in fish. Published by Elsevier Ltd. C1 [Eagles-Smith, Collin A.; Herring, Garth; Johnson, Branden] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis Res Grp, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Graw, Rick] US Forest Serv, USDA, Washington, DC USA. RP Eagles-Smith, CA (reprint author), US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis Res Grp, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM ceagles-smith@usgs.gov FU U.S. Department of Agriculture Forest Service; U.S. Geological Survey Contaminant Biology Program FX This study was funded by the U.S. Department of Agriculture Forest Service, and the U.S. Geological Survey Contaminant Biology Program. This study would not have been possible without many dedicated field hours from a number of people. In particular, we appreciate the support of David Salo, Alan Miller, Bob Rock, Leigh Woodruff, Mary Ellen Emerick, Holly Akenson, Amy Busch, Randi Jandt, Tim Bailey, and Jeff Yanke. We also are grateful for the efforts of Kevin Donner, John Pierce, Brandon Kowalski, and Nick Baker for their laboratory efforts; and Patti Haggerty for her expertise and guidance on geospatial analyses. We also thank David Salo, Alan Miller, Martin Fitzpatrick, Leigh Woodruff, Gene Foster, and two anonymous reviewers for comments on previous versions of the report. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 51 TC 5 Z9 5 U1 5 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD MAY PY 2016 VL 212 BP 279 EP 289 DI 10.1016/j.envpol.2016.01.049 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN1BA UT WOS:000376800000033 PM 26854697 ER PT J AU Shao, DY Zhang, LJ Du, SK Yokoyama, W Shi, JL Li, N Wang, JX AF Shao, Dongyan Zhang, Lijuan Du, Shuangkui Yokoyama, Wallace Shi, Junling Li, Nan Wang, Jixun TI Polyphenolic Content and Color of Seedless and Seeded Shade Dried Chinese Raisins SO FOOD SCIENCE AND TECHNOLOGY RESEARCH LA English DT Article DE Raisin; polyphenol; color; seeded status; antioxidant capacity ID ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; EXTRACTS; REGIONS; FOODS AB 29 grape cultivars, of seeded and seedless raisins from black, reddish-brown and yellowish-green grape varieties were examined to determine the relationship between grape color, seeded status and raisin phenolic content. The raisins were analyzed for phenolic profile, total phenolic (TP) and flavonoid contents (TF), and antioxidant activities (DPPH, ABTS and FRAP). We found that despite the perception that dark colored raisins, contain higher levels of polyphenolic antioxidants, only seedless raisins from black grapes were higher in TP. Black raisins exhibited significant higher values of TP and FRAP activity than reddish-brown and yellowish-green raisins. The values of TP, TF, DPPH, ABTS, and FRAP capacities for seeded raisins were higher than those of seedless samples. Raisins could be classified into three groups and some raisins differing in seed-bearing status or color were grouped together, indicating the antioxidant properties of raisins were not only determined by raisin color, but also by their seed bearing status. C1 [Shao, Dongyan; Shi, Junling; Li, Nan] Northwestern Polytech Univ, Sch Life Sci, Key Lab Space Biosci & Biotechnol, 127 Youyi West Rd, Xian 710072, Shaanxi, Peoples R China. [Zhang, Lijuan; Du, Shuangkui] Northwest A&F Univ, Coll Food Sci & Engn, 28 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China. [Yokoyama, Wallace] USDA, ARS, Proc Foods Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Wang, Jixun] Xinjiang Acad Agr Sci, Inst Hort, Saybag Urumqi 830091, Xinjiang, Peoples R China. RP Shi, JL (reprint author), Northwestern Polytech Univ, Sch Life Sci, Key Lab Space Biosci & Biotechnol, 127 Youyi West Rd, Xian 710072, Shaanxi, Peoples R China. EM sj1shi2004@nwpu.edu.cn FU Ministry of Agriculture of the People's Republic of China through the funds of Agro-scientific Research in the Public Interest [201003021]; Modern Agricultural Industry Technology System [CAR-30]; project of Shaanxi Provincial Natural Science Foundation [20157Q3083]; Fundamental Research Funds for the Central Universities [3102014JCQ15011]; China Postdoctoral Science Foundation [2014M562451] FX The authors acknowledge financial support from the Ministry of Agriculture of the People's Republic of China through the funds of Agro-scientific Research in the Public Interest (No. 201003021) and Modern Agricultural Industry Technology System (No. CAR-30) and support from the project of Shaanxi Provincial Natural Science Foundation (No.20157Q3083), Fundamental Research Funds for the Central Universities (No. 3102014JCQ15011) and the China Postdoctoral Science Foundation (No. 2014M562451). NR 25 TC 0 Z9 0 U1 19 U2 22 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1344-6606 J9 FOOD SCI TECHNOL RES JI Food Sci. Technol. Res. PD MAY PY 2016 VL 22 IS 3 BP 359 EP 369 DI 10.3136/fstr.22.359 PG 11 WC Food Science & Technology SC Food Science & Technology GA DN1GV UT WOS:000376815200007 ER PT J AU Havill, NP Shiyake, S Galloway, AL Foottit, RG Yu, GY Paradis, A Elkinton, J Montgomery, ME Sano, M Caccone, A AF Havill, Nathan P. Shiyake, Shigehiko Galloway, Ashley Lamb Foottit, Robert G. Yu, Guoyue Paradis, Annie Elkinton, Joseph Montgomery, Michael E. Sano, Masakazu Caccone, Adalgisa TI Ancient and modern colonization of North America by hemlock woolly adelgid, Adelges tsugae (Hemiptera: Adelgidae), an invasive insect from East Asia SO MOLECULAR ECOLOGY LA English DT Article DE complex life cycle; cyclical parthenogenesis; host range; invasive species ID APPROXIMATE BAYESIAN COMPUTATION; DNA-SEQUENCE DATA; INTERSPECIFIC VARIATION; HOST SPECIALIZATION; POPULATION-GENETICS; MICROSATELLITE DATA; PROPAGULE PRESSURE; MITOCHONDRIAL-DNA; BORER COLEOPTERA; UNITED-STATES AB Hemlock woolly adelgid, Adelges tsugae, is an invasive pest of hemlock trees (Tsuga) in eastern North America. We used 14 microsatellites and mitochondrial COI sequences to assess its worldwide genetic structure and reconstruct its colonization history. The resulting information about its life cycle, biogeography and host specialization could help predict invasion by insect herbivores. We identified eight endemic lineages of hemlock adelgids in central China, western China, Ulleung Island (South Korea), western North America, and two each in Taiwan and Japan, with the Japanese lineages specializing on different Tsuga species. Adelgid life cycles varied at local and continental scales with different sexual, obligately asexual and facultatively asexual lineages. Adelgids in western North America exhibited very high microsatellite heterozygosity, which suggests ancient asexuality. The earliest lineages diverged in Asia during Pleistocene glacial periods, as estimated using approximate Bayesian computation. Colonization of western North America was estimated to have occurred prior to the last glacial period by adelgids directly ancestral to those in southern Japan, perhaps carried by birds. The modern invasion from southern Japan to eastern North America caused an extreme genetic bottleneck with just two closely related clones detected throughout the introduced range. Both colonization events to North America involved host shifts to unrelated hemlock species. These results suggest that genetic diversity, host specialization and host phylogeny are not predictive of adelgid invasion. Monitoring non-native sentinel host trees and focusing on invasion pathways might be more effective methods of preventing invasion than making predictions using species traits or evolutionary history. C1 [Havill, Nathan P.; Montgomery, Michael E.] US Forest Serv, No Res Stn, USDA, Hamden, CT 06514 USA. [Shiyake, Shigehiko] Osaka Museum Nat Hist, Osaka 5460034, Japan. [Galloway, Ashley Lamb] Virginia Polytech Inst & State Univ, Dept Entomol, Blacksburg, VA 24061 USA. [Foottit, Robert G.] Agr & Agri Food Canada, Canadian Natl Collect Insects, Ottawa, ON K1A 0C6, Canada. [Yu, Guoyue] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China. [Paradis, Annie; Elkinton, Joseph] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Sano, Masakazu] Hokkaido Univ, Grad Sch Agr, Systemat Entomol, Sapporo, Hokkaido 0608589, Japan. [Caccone, Adalgisa] Yale Univ, Dept Ecol & Evolutionary Biol, New Haven, CT 06520 USA. [Galloway, Ashley Lamb] Roane State Community Coll, Dept Biol, Oak Ridge, TN 37830 USA. RP Havill, NP (reprint author), US Forest Serv, No Res Stn, USDA, Hamden, CT 06514 USA. EM nphavill@fs.fed.us FU USDA NIFA Award [2007-35302-18097]; US Forest Service Northern and Southern Research Stations; Yale Institute for Biospheric Studies FX This project was supported by USDA NIFA Award 2007-35302-18097, the US Forest Service Northern and Southern Research Stations and the Yale Institute for Biospheric Studies. Andrea Gloria-Soria, Talbot Trotter and Sandy Liebhold provided comments on an early draft. We are grateful to Eric Maw who helped with laboratory work, to Darcy Nelson, with US Forest Service International Programs, who facilitated collecting logistics, and to our many colleagues who collected samples for this project. NR 103 TC 2 Z9 2 U1 27 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD MAY PY 2016 VL 25 IS 9 BP 2065 EP 2080 DI 10.1111/mec.13589 PG 16 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DN4GZ UT WOS:000377023400013 PM 26880353 ER PT J AU Stocker, MD Rodriguez-Valentin, JG Pachepsky, YA Shelton, DR AF Stocker, M. D. Rodriguez-Valentin, J. G. Pachepsky, Y. A. Shelton, D. R. TI Spatial and temporal variation of fecal indicator organisms in two creeks in Beltsville, Maryland SO WATER QUALITY RESEARCH JOURNAL OF CANADA LA English DT Article DE diurnal variation; hyporheic exchange; indicator organisms; water monitoring; water quality ID MICROBIAL WATER-QUALITY; LAND-USE PRACTICES; ESCHERICHIA-COLI; SURFACE-WATER; BACTERIA; RIVER; STREAM; VARIABILITY; CONTAMINATION; ENVIRONMENT AB Evaluation of microbial water quality is commonly based on monitoring populations of fecal indicator organisms (FIO) such as Escherichia coli (EC) and enterococci (ENT). The occurrence of elevated FIO concentrations in surface waters after storm events is well documented and has been attributed to runoff and sediment resuspension. The reasons for FIO concentration variation under baseflow conditions are less clear. The objective of this study was to quantify the variability of EC and ENT in two small streams running through agricultural land use areas. FIO concentrations were measured at upstream and downstream locations under baseflow conditions. Concentrations were not significantly different along cross-sections of the streams. Diurnal concentration trends were observed at each of the sampling locations. Significant differences in concentrations between upstream and downstream locations were noted for both creeks during baseflow periods when no runoff or sediment resuspension occurred. A hypothetical explanation is that indicator organisms are released from sediments during baseflow conditions due to the effect of groundwater influx into streams or due to the motility of indicator organisms. If confirmed, this hypothesis may affect our understanding of the role of sediments in the microbial quality of surface waters. C1 [Stocker, M. D.; Pachepsky, Y. A.; Shelton, D. R.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD USA. [Rodriguez-Valentin, J. G.] Interamer Univ Puerto Rico, Dept Biol Chem & Environm Sci, San German, PR USA. RP Stocker, MD (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD USA. EM matthew.stocker@ars.usda.gov NR 60 TC 3 Z9 3 U1 3 U2 4 PU IWA PUBLISHING PI LONDON PA ALLIANCE HOUSE, 12 CAXTON ST, LONDON SW1H0QS, ENGLAND SN 1201-3080 J9 WATER QUAL RES J CAN JI Water Qual. Res. J. Canada PD MAY PY 2016 VL 51 IS 2 BP 167 EP 179 DI 10.2166/wqrjc.2016.044 PG 13 WC Water Resources SC Water Resources GA DN6RF UT WOS:000377202800008 ER PT J AU Zumba, J Rodgers, J AF Zumba, Jimmy Rodgers, James TI Cotton Micronaire Measurements Using Small Portable Near-Infrared (NIR) Analyzers SO APPLIED SPECTROSCOPY LA English DT Article DE Micronaire; cotton micronaire; micronaire measurement; near-infrared (NIR) spectroscopy; portable NIR; JDSU MicroNIR ID FIBER MICRONAIRE; QUALITY; SPECTROSCOPY; REFLECTANCE; MATURITY AB A key quality and processing parameter for cotton fiber is micronaire, which is a function of the fiber's maturity and fineness. Near-infrared (NIR) spectroscopy has previously shown the ability to measure micronaire, primarily in the laboratory and using large, research-grade laboratory NIR instrumentation. International interest has been expressed by the industry in the measurement of fiber micronaire using small, portable NIR spectroscopy instruments for both laboratory and outside the laboratory (e.g., field or greenhouse) locations. New, very small NIR micro-spectrometers have been commercialized that offer the potential advantages of smaller size and lower weight, lower cost, and increased portability over current portable units. A program was implemented to determine the feasibility of a small NIR micro-spectrometer to measure fiber micronaire both in the laboratory and outside the laboratory, with initial emphasis on laboratory measurements prior to moving to field evaluations. In the laboratory, distinct spectral differences with increasing micronaire were observed. Optimal sampling and instrumental procedures and protocols for two units (different spectral wavelength capabilities) were established. Comparative evaluations established very good method micronaire agreement between the micro-spectrometer and a standard portable spectrometer, with high Regression (R) value, low residuals, and few outliers (less than 20%). The NIR micro-spectrometer measurements were fast (<1min per sample), required no sample preparation, and were easy to perform. All end-state criteria were exceeded. The rapid and accurate laboratory measurement of fiber micronaire with a NIR micro-spectrometer was demonstrated. C1 [Zumba, Jimmy; Rodgers, James] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Zumba, J (reprint author), ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM jimmy.zumba@ars.usda.gov NR 34 TC 1 Z9 1 U1 4 U2 7 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0003-7028 EI 1943-3530 J9 APPL SPECTROSC JI Appl. Spectrosc. PD MAY PY 2016 VL 70 IS 5 SI SI BP 794 EP 803 DI 10.1177/0003702816638227 PG 10 WC Instruments & Instrumentation; Spectroscopy SC Instruments & Instrumentation; Spectroscopy GA DM3XU UT WOS:000376280800008 PM 26988659 ER PT J AU Al-Gaadi, KA Patil, VC Tola, E Madugundu, R Gowda, PH AF Al-Gaadi, K. A. Patil, V. C. Tola, E. Madugundu, R. Gowda, P. H. TI Evaluation of METRIC-derived ET fluxes over irrigated alfalfa crop in desert conditions using scintillometer measurements SO ARABIAN JOURNAL OF GEOSCIENCES LA English DT Article DE Centre pivot system; Evapotranspiration; Landsat-8 data; Alfalfa field; Surface layer scintillometer ID LARGE-APERTURE SCINTILLOMETER; SURFACE-ENERGY BALANCE; SENSIBLE HEAT-FLUX; MAPPING EVAPOTRANSPIRATION; TEMPERATURE; MODELS; AREA; SCINTILLATION; UNCERTAINTY; CALIBRATION AB A field study on a 50-ha alfalfa (Medicago sativa L.) irrigated field was conducted to investigate the performance of the remote sensing (RS) based Mapping EvapoTranspiration at high Resolution with Internalized Calibration (METRIC) model in the estimation of evapotranspiration (ET) under the arid conditions of Saudi Arabia. The METRIC model performance was investigated by comparing the energy fluxes estimated by the model to the output of a surface layer scintillometer (SLS) system installed in the field, given the fact that the SLS is efficient in measuring sensible heat fluxes (H) over vegetative areas. Landsat-8 reflectance data were used as inputs for the METRIC model. Results of the study revealed that the H-METRIC data was strongly correlated with the H-SLS data with an R-2 value of 0.74 (P > F = 0.0064) and a mean bias error (MBE) of 6.05 W m(-2) (6 %). The METRIC model showed a good performance in estimating the hourly latent heat (LE) fluxes compared with SLS data with an R2 value of 0.81 (P > F = 0.0023), an MBE of 24.46 W m(-2) (8 %) and a Nash-Sutcliffe efficiency (NSE) of 0.91. Furthermore, the hourly ET was estimated with an MBE and an NSE of 0.036 mm h(-1) (8 %) and 1.00, respectively. Compared to the SLS data, the METRIC model was found to generally provide an efficient and an accurate means of energy fluxes estimation; therefore, ET estimation over the studied irrigated alfalfa crop. C1 [Al-Gaadi, K. A.; Patil, V. C.; Tola, E.; Madugundu, R.] King Saud Univ, Precis Agr Res Chair, Riyadh, Saudi Arabia. [Al-Gaadi, K. A.] King Saud Univ, Coll Food & Agr Sci, Dept Agr Engn, Riyadh, Saudi Arabia. [Patil, V. C.] Edith Cowan Univ, Electron Sci Res Inst, Joondalup, Australia. [Gowda, P. H.] USDA ARS, Forage & Livestock Prod Res Unit, Grazing Lands Res Lab, El Reno, OK USA. RP Madugundu, R (reprint author), King Saud Univ, Precis Agr Res Chair, Riyadh, Saudi Arabia. EM rmadugundu@ksu.edu.sa RI Madugundu, Rangaswamy/N-9214-2013 OI Madugundu, Rangaswamy/0000-0002-5326-4785 FU King Saud University, Vice Deanship of Research Chairs FX This project was financially supported by King Saud University, Vice Deanship of Research Chairs. The assistance provided by the graduate students M.E. Abass, A.M. Zeyada, and A.G. Kayad in the accomplishment of the field research work was quite valuable. The unstinted cooperation and support extended by Mr. Jack King, Mr. Alan King, and their team in carrying out the research are gratefully acknowledged. NR 52 TC 0 Z9 0 U1 3 U2 11 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1866-7511 EI 1866-7538 J9 ARAB J GEOSCI JI Arab. J. Geosci. PD MAY PY 2016 VL 9 IS 6 AR 441 DI 10.1007/s12517-016-2469-8 PG 12 WC Geosciences, Multidisciplinary SC Geology GA DM8KH UT WOS:000376611000015 ER PT J AU Pearson, DE Ortega, YK Runyon, JB Butler, JL AF Pearson, Dean E. Ortega, Yvette K. Runyon, Justin B. Butler, Jack L. TI Secondary invasion: The bane of weed management SO BIOLOGICAL CONSERVATION LA English DT Review DE Invasive plant; Invasion treadmill; Noxious weed; Restoration; Revegetation; Reseeding ID ENEMY RELEASE HYPOTHESIS; BIOLOGICAL INVASIONS; PLANT-COMMUNITIES; NITROGEN-CYCLE; UNITED-STATES; NATIVE PLANTS; NATIONAL-PARK; LEAFY SPURGE; SOUTH-AFRICA; RESTORATION AB Exotic plant invasions present a global threat to natural ecosystems, yet the efficacy of management efforts in mitigating invader impacts remains unclear. A rapidly emerging problem is that of secondary invasion - an increase in abundance of non-target exotics following treatment of targeted invasive plants. Here, we present a global literature review and meta-analysis directed at quantifying the magnitude of secondary invasion effects and identifying possible causes. Of 168 studies examining the efficacy of exotic plant management in terrestrial habitats, 29% quantified community responses sufficiently to evaluate secondary invasion. Meta-analysis of 60 cases from 38 studies showed that control efforts strongly reduced target invader abundance overall, but secondary invaders increased, with a mean effect size double what was found for native plants, which increased only weakly. Moreover, 89% of the secondary invaders identified were classified as noxious or invasive plants. Increases in secondary invaders were correlated with target invader reductions, but control method and target invader growth form failed to explain variation in secondary invader responses. These results suggest that target invader suppression is the key factor driving release of secondary invaders. However, management side effects, target invader legacy effects, provenance effects, and shifting environmental conditions may all facilitate secondary invasion. Invasive plant management often successfully suppresses target invaders, but the result is largely secondary invasion. Addressing this problem requires management strategies that anticipate and suppress secondary invaders while rapidly restoring native plants to fill the space vacated by the target weed. Accomplishing the latter will require improved re-vegetation techniques. Published by Elsevier B.V. C1 [Pearson, Dean E.; Ortega, Yvette K.] US Forest Serv, Rock Mt Res Stn, USDA, Missoula, MT USA. [Pearson, Dean E.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Runyon, Justin B.] US Forest Serv, Rocky Mt Res Stn, USDA, Bozeman, MT USA. [Butler, Jack L.] US Forest Serv, Rocky Mt Res Stn, USDA, Rapid City, SD USA. RP Pearson, DE (reprint author), US Forest Serv, Rock Mt Res Stn, USDA, Missoula, MT USA. EM dpearson@fs.fed.us FU USDA FS, Rocky Mountain Research Station FX We thank Peter Lesica and Bryce Christiaens for helpful comments on the manuscript. Jennifer Birdsall conducted the literature review, and Hilary Schultz help with manuscript preparation. This work was funded by the USDA FS, Rocky Mountain Research Station. NR 85 TC 1 Z9 1 U1 30 U2 47 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD MAY PY 2016 VL 197 BP 8 EP 17 DI 10.1016/j.biocon.2016.02.029 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DN1AS UT WOS:000376798800002 ER PT J AU Ji, T Baik, BK AF Ji, Taehyun Baik, Byung-Kee TI Storage Conditions Affecting Increase in Falling Number of Soft Red Winter Wheat Grain SO CEREAL CHEMISTRY LA English DT Article ID ALPHA-AMYLASE ACTIVITY; RAPID METHOD; CULTIVAR; QUALITY AB Falling number (FN) of wheat grain, a measure of preharvest sprouting, tends to increase during storage; however, grain and storage conditions that impact FN changes are poorly understood. Wheat grain samples of varying FN from several cultivars were obtained by artificial sprouting, by incubating wheat stalks, or directly from the field and were used to determine the effects of cultivar, storage temperature, grain moisture content, and initial FN on changes in FN and alpha-amylase activity. Increases in FN of artificially sprouted grain during storage were affected by temperature but not evidently by grain moisture in the range of 10.0-13.0%. The FN of artificially sprouted grain increased when stored at 5, 23, and 35 degrees C for 20 weeks by averages of 9.4, 24.1, and 34.4, respectively. The influences of storage temperature and initial FN of grain obtained from incubated stalks were different between cultivars when stored for 8 weeks. Wheat grain obtained directly from the field also exhibited significant increases in FN during 8 weeks of storage at 5, 23, and 35 degrees C with average increases of 10.0, 27.1, and 38.5, respectively. The impact of alpha-amylase activity on the increase in FN during storage was evident only for fieldharvested grain of varying FN. alpha-Amylase activity exhibited a negative logarithmic relationship (R-2 = 0.87) with FN in field-harvested grain. The magnitude of the changes in alpha-amylase activity varied by cultivar. C1 [Ji, Taehyun; Baik, Byung-Kee] ARS, USDA, CSWQRU, Soft Wheat Qual Lab, 1680 Madison Ave, Wooster, OH 44691 USA. RP Baik, BK (reprint author), ARS, USDA, CSWQRU, Soft Wheat Qual Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM byungkee.baik@ars.usda.gov FU Maryland Grain Producers Utilization Board FX The authors acknowledge the staff of the USDA-ARS Soft Wheat Quality Laboratory, Tony Karcher, Scott Beil, Fengyun Ma, Anne Sturbaum, Sharon Croskey, Tom Donelson, and Amy Bugaj for their assistance in conducting the experiments. The financial support of the Maryland Grain Producers Utilization Board is gratefully acknowledged. NR 20 TC 0 Z9 0 U1 7 U2 11 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2016 VL 93 IS 3 BP 263 EP 267 DI 10.1094/CCHEM-08-15-0167-R PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DM6CU UT WOS:000376438500007 ER PT J AU Moreau, RA Fang, XZ AF Moreau, Robert A. Fang, Xuezhi TI Analysis of Alkylresorcinols in Wheat Germ Oil and Barley Germ Oil via HPLC and Fluorescence Detection: Cochromatography with Tocols SO CEREAL CHEMISTRY LA English DT Article ID HULLESS BARLEY; PRODUCTS; SCARIFICATION; FRACTIONS AB Alkylresorcinols are long-chain phenolic compounds that have been reported to be localized in the outer layers of the kernels of wheat, rye, barley, and other grains. A sensitive HPLC method with fluorescence detection was recently reported for the quantitative analysis of alkylresorcinols in cereal grains and products. Using this new HPLC method we report for the first time that wheat germ oil contains moderate levels of alkylresorcinols, approximately 800-1,500 mu g per gram of oil. We also found that commercial wheat germ oil and some experimental samples of wheat germ oil and barley germ oil also contained three unknown peaks. Upon further evaluation of these peaks it was found that the peaks appeared to be tocopherols (one peak of alpha-tocopherol, one peak of delta-tocopherol, and a peak with the combination of beta- and gamma-tocopherol), even though the excitation and emission wavelengths for alkylresorcinols (excitation 274 nm and emission 300 nm) are different than those for tocols (excitation 294 nm and emission 326 nm). We also found that with this HPLC system one alkylresorcinol, AR17, had the same retention time (7.6 min) as delta-tocotrienol and that another alkylresorcinol, AR19, had the same retention time (10.8 min) as alpha-tocotrienol. C1 [Moreau, Robert A.] ARS, USDA, Eastern Reg Res Ctr, Sustainable Biofuels & Coprod Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Fang, Xuezhi] Chinese Acad Forestry, State Forestry Adm, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China. RP Moreau, RA (reprint author), ARS, USDA, Eastern Reg Res Ctr, Sustainable Biofuels & Coprod Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM robert.moreau@ars.usda.gov OI Moreau, Robert/0000-0002-8166-8322 NR 6 TC 0 Z9 0 U1 1 U2 2 PU AACC INTERNATIONAL PI ST PAUL PA 3340 PILOT KNOB RD, ST PAUL, MN 55121-2097 USA SN 0009-0352 EI 1943-3638 J9 CEREAL CHEM JI Cereal Chem. PD MAY-JUN PY 2016 VL 93 IS 3 BP 293 EP 298 DI 10.1094/CCHEM-07-15-0141-R PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DM6CU UT WOS:000376438500011 ER PT J AU Seymour, M Perera, OP Fescemyer, HW Jackson, RE Fleischer, SJ Abel, CA AF Seymour, Mathew Perera, Omaththage P. Fescemyer, Howard W. Jackson, Ryan E. Fleischer, Shelby J. Abel, Craig A. TI Peripheral genetic structure of Helicoverpa zea indicates asymmetrical panmixia SO ECOLOGY AND EVOLUTION LA English DT Article DE DAPC; Helicoverpa; heliothine; moth; population genetics; stable isotopes ID LONG-DISTANCE MIGRATION; ARMIGERA LEPIDOPTERA; HELIOTHIS-ZEA; CORN-EARWORM; R-PACKAGE; POPULATION-STRUCTURE; NATURAL-POPULATIONS; GENOTYPING ERRORS; HOST USE; NOCTUIDAE AB Seasonal climatic shifts create peripheral habitats that alternate between habitable and uninhabitable for migratory species. Such dynamic peripheral habitats are potential sites where migratory species could evolve high genetic diversity resulting from convergence of immigrants from multiple regionally distant areas. Migrant populations of Helicoverpa zea (Boddie) captured during two different seasons were assessed for genetic structure using microsatellite markers and for host plant type using stable carbon isotope analysis. Individuals (N = 568) were genotyped and divided into 13 putative populations based on collection site and time. Fixation indices (F-statistics), analysis of molecular variance (AMOVA), and discriminant analysis of principal components (DAPC) were used to examine within and among population genetic variation. Mean number of alleles per locus was 10.25 (+/- 3.2 SD), and allelic richness ranged from 2.38 to 5.13 (+/- 3.2 SD). The observed and expected heterozygosity ranged from 0.07 to 0.48 and 0.08 to 0.62, respectively. Low F-ST (0.01 to 0.02) and high F-IS (0.08 to 0.33) values suggest captured migrants originated from breeding populations with different allele frequencies. We postulate that high genetic diversity within migrant populations and low genetic differentiation among migrant populations of H. zea are the result of asymmetrical immigration due to the high dispersal and reproductive behavior of H. zea, which may hinder the adaptation and establishment of H. zea to peripheral habitat. These findings highlight the importance of assessing peripheral population structure in relation to ecological and evolutionary dynamics of this and other highly reproductive and dispersive species. C1 [Seymour, Mathew; Perera, Omaththage P.; Jackson, Ryan E.] USDA ARS, Southern Insect Management Res Unit, Stoneville, MS 38776 USA. [Fescemyer, Howard W.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Fleischer, Shelby J.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Abel, Craig A.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Seymour, Mathew] Bangor Univ, Sch Biol Sci, Mol Ecol & Fisheries Genet Lab, Bangor LL57 2UW, Gwynedd, Wales. [Jackson, Ryan E.] Syngenta Crop Protect, 410 South Swing Rd, Greensboro, NC 27409 USA. RP Perera, OP (reprint author), Southern Insect Management Res Unit, 141 Expt Stn Rd,POB 346, Stoneville, MS 38776 USA. EM op.perera@ars.usda.gov FU USDA-ARS [58-6402-5-066] FX Funding for work performed by HWF and SJF was from USDA-ARS Specific Cooperative Agreement 58-6402-5-066. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 67 TC 0 Z9 0 U1 2 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD MAY PY 2016 VL 6 IS 10 BP 3198 EP 3207 DI 10.1002/ece3.2106 PG 10 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DM8XB UT WOS:000376646700013 PM 27096078 ER PT J AU Humphrey, PT Gloss, AD Alexandre, NM Villalobos, MM Fremgen, MR Groen, SC Meihls, LN Jander, G Whiteman, NK AF Humphrey, Parris T. Gloss, Andrew D. Alexandre, Nicolas M. Villalobos, Martha M. Fremgen, Marcella R. Groen, Simon C. Meihls, Lisa N. Jander, Georg Whiteman, Noah K. TI Aversion and attraction to harmful plant secondary compounds jointly shape the foraging ecology of a specialist herbivore SO ECOLOGY AND EVOLUTION LA English DT Article DE Drosophila; glucosinolate; inducible defense; jasmonic acid; oviposition; preference performance ID CRUCIFER CARDAMINE-CORDIFOLIA; NATIVE CRUCIFER; INSECT HERBIVORY; LEPIDOPTERAN HERBIVORES; PSEUDOMONAS-SYRINGAE; LEAF MINER; ARABIDOPSIS; DEFENSE; EVOLUTION; INDOLE AB Most herbivorous insect species are restricted to a narrow taxonomic range of host plant species. Herbivore species that feed on mustard plants and their relatives in the Brassicales have evolved highly efficient detoxification mechanisms that actually prevent toxic mustard oils from forming in the bodies of the animals. However, these mechanisms likely were not present during the initial stages of specialization on mustard plants similar to 100 million years ago. The herbivorous fly Scaptomyza nigrita (Drosophilidae) is a specialist on a single mustard species, bittercress (Cardamine cordifolia; Brassicaceae) and is in a fly lineage that evolved to feed on mustards only in the past 10-20 million years. In contrast to many mustard specialists, S. nigrita does not prevent formation of toxic breakdown products (mustard oils) arising from glucosinolates (GLS), the primary defensive compounds in mustard plants. Therefore, it is an appealing model for dissecting the early stages of host specialization. Because mustard oils actually form in the bodies of S. nigrita, we hypothesized that in lieu of a specialized detoxification mechanism, S. nigrita may mitigate exposure to high GLS levels within plant tissues using behavioral avoidance. Here, we report that jasmonic acid (JA) treatment increased GLS biosynthesis in bittercress, repelled adult female flies, and reduced larval growth. S. nigrita larval damage also induced foliar GLS, especially in apical leaves, which correspondingly displayed the least S. nigrita damage in controlled feeding trials and field surveys. Paradoxically, flies preferred to feed and oviposit on GLS-producing Arabidopsis thaliana despite larvae performing worse in these plants versus non-GLS-producing mutants. GLS may be feeding cues for S. nigrita despite their deterrent and defensive properties, which underscores the diverse relationship a mustard specialist has with its host when lacking a specialized means of mustard oil detoxification. C1 [Humphrey, Parris T.; Gloss, Andrew D.; Alexandre, Nicolas M.; Villalobos, Martha M.; Groen, Simon C.; Whiteman, Noah K.] Univ Arizona, Ecol & Evolutionary Biol, Tucson, AZ 85721 USA. [Humphrey, Parris T.; Gloss, Andrew D.; Alexandre, Nicolas M.; Villalobos, Martha M.; Fremgen, Marcella R.; Groen, Simon C.; Whiteman, Noah K.] Rocky Mt Biol Labs, Gothic, CO 81224 USA. [Meihls, Lisa N.; Jander, Georg] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [Humphrey, Parris T.] Harvard Univ, Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Whiteman, Noah K.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Meihls, Lisa N.] USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Humphrey, PT (reprint author), Harvard Univ, Organism & Evolutionary Biol, Cambridge, MA 02138 USA.; Whiteman, NK (reprint author), Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. EM phumphrey@fas.harvard.edu; whiteman@berkeley.edu RI Groen, Simon/G-2443-2012 FU Division of Integrative Organismal Systems [1139329, 1339237]; Agricultural Research Service [2011-67012-30675]; Division of Environmental Biology [1256758, 1309493, 1405966]; Templeton Foundation [41855] FX Division of Integrative Organismal Systems (Grant/Award Number: 1139329, 1339237) Agricultural Research Service (Grant/Award Number: 2011-67012-30675) Division of Environmental Biology (Grant/Award Number: 1256758, 1309493, 1405966) The Templeton Foundation (Award Number : 41855). NR 50 TC 1 Z9 1 U1 8 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD MAY PY 2016 VL 6 IS 10 BP 3256 EP 3268 DI 10.1002/ece3.2082 PG 13 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DM8XB UT WOS:000376646700018 PM 27096082 ER PT J AU Davies, KW Bates, JD Boyd, CS Svejcar, TJ AF Davies, Kirk W. Bates, Jon D. Boyd, Chad S. Svejcar, Tony J. TI Prefire grazing by cattle increases postfire resistance to exotic annual grass (Bromus tectorum) invasion and dominance for decades SO ECOLOGY AND EVOLUTION LA English DT Article DE Artemisia; cheatgrass; disturbance; exotic annual grass; fire-grazing interaction; herbivory; invasive plants; sagebrush ID BIOLOGICAL SOIL CRUSTS; SAGEBRUSH PLANT-COMMUNITIES; WESTERN NORTH-AMERICA; SUB-ALPINE FOREST; GREAT-BASIN; ALIEN PLANTS; SHRUB-STEPPE; VEGETATION CHARACTERISTICS; WILDFIRE ACTIVITY; AFRICAN SAVANNA AB 1 Fire, herbivory and their interaction influence plant community dynamics. However, little is known about the influence of prefire herbivory on postfire plant community response, particularly long-term resistance to postfire exotic plant invasion in areas that historically experienced limited large herbivore pressure and infrequent, periodic fires. 2 We investigated the long-term postfire effects of prefire herbivory by cattle, an exotic herbivore, in Artemisia (sagebrush) plant communities in the northern Great Basin, USA. Study areas were moderately grazed or not grazed by cattle since 1936 and then were burned in 1993. Plant community response was measured the 19th through the 22nd year postfire. Prior to burning exotic annual grass presence was minimal (<0.5% foliar cover) and plant community characteristics were similar between grazed and ungrazed treatments, with the exception of litter biomass being two times greater in the ungrazed treatment. 3 Two decades postfire, Bromus tectorum L., an exotic annual grass, dominated the ungrazed treatment. Native bunchgrasses, species richness, and soil biological crusts were greater in prefire grazed areas compared to ungrazed areas. 4 These results suggest that moderate prefire herbivory by cattle increased the resistance of the plant community to postfire invasion and dominance by B. tectorum. We presume that herbivory reduced mortality of large perennial bunchgrasses during the fire by reducing fine fuel (litter) and subsequently burn temperatures. 5 Synthesis: This research demonstrates that a moderate disturbance (herbivory) may mediate the effects of a subsequent disturbance (fire). The effects of disturbances are not independent; therefore quantifying these interactions is critical to preventing oversimplification of complex plant community dynamics and guiding the conservation of endangered ecosystems. C1 [Davies, Kirk W.; Bates, Jon D.; Boyd, Chad S.; Svejcar, Tony J.] USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR USA. RP Davies, KW (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR USA.; Davies, KW (reprint author), EOARC, 67826-A Hwy 205, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu NR 96 TC 0 Z9 0 U1 19 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD MAY PY 2016 VL 6 IS 10 BP 3356 EP 3366 DI 10.1002/ece3.2127 PG 11 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA DM8XB UT WOS:000376646700026 PM 27103990 ER PT J AU Lujaji, FC Boateng, AA Schaffer, MA Mullen, CA Mkilaha, ISN Mtui, PL AF Lujaji, Frank C. Boateng, Akwasi A. Schaffer, Mark A. Mullen, Charles A. Mkilaha, Iddi S. N. Mtui, Peter L. TI Pyrolysis Oil Combustion in a Horizontal Box Furnace with an Externally Mixed Nozzle SO ENERGY & FUELS LA English DT Article; Proceedings Paper CT 16th International Conference on Petroleum Phase Behavior and Fouling (PetroPhase) CY JUN 07-11, 2015 CL Playa del Carmen, MEXICO ID STABILIZED SWIRL BURNER; LIQUID-ETHANOL BLEND; SPRAY COMBUSTION; BIO-OIL; LIGNOCELLULOSIC BIOMASS; OIL/ETHANOL BLENDS; GASEOUS EMISSIONS; PERFORMANCE AB Combustion characteristics of neat biomass fast-pyrolysis oil were studied in a horizontal combustion chamber with a rectangular cross-section. An air-assisted externally mixed nozzle known to successfully atomize heavy fuel oils was installed in a modified 100 kW (350 000 BTU/h nominal capacity) burner to explore full utility for pyrolysis oil (bio-oil) combustion in a furnace. Combustion experiments were conducted at air/fuel equivalence ratios of 0.46, 0.53, and 0.68 (116, 88, and 47% excess air, respectively) and compared to diesel fuel flames (control) at the two higher air/fuel equivalence ratios. In these experiments, the fuel flow rate was maintained at a constant energy input (equivalent of 24 kW(th)). The results revealed that, while the externally mixed nozzle could effectively atomize and ensure stable combustion of neat bio-oil at the set heat rate, this comes with a penalty associated with a lower peak flame temperature and, hence, heat flux. The formation of carbon monoxide (CO) decreases with an increasing air/fuel equivalence ratio for bio-oil combustion. The levels of carbon dioxide (CO2) and nitrogen oxides (NOx) increase with an increasing air/fuel equivalence ratio for bio-oil combustion and were slightly higher than that generated by diesel. Hydrocarbon emissions do not follow any defined trend with an increasing air/fuel equivalence ratio for bio-oil, as typically observed for diesel fuels as a result of the oxygenated nature of bio-oil. C1 [Lujaji, Frank C.; Boateng, Akwasi A.; Schaffer, Mark A.; Mullen, Charles A.] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Lujaji, Frank C.; Mkilaha, Iddi S. N.; Mtui, Peter L.] Nelson Mandela African Inst Sci & Technol, POB 447, Arusha, Tanzania. RP Boateng, AA (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM akwasi.boateng@ars.usda.gov NR 29 TC 1 Z9 1 U1 4 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD MAY PY 2016 VL 30 IS 5 BP 4126 EP 4136 DI 10.1021/acs.energyfuels.6b00318 PG 11 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DM5VF UT WOS:000376417600063 ER PT J AU Walker, DA Daniels, FJA Alsos, I Bhatt, US Breen, AL Buchhorn, M Bultmann, H Druckenmiller, LA Edwards, ME Ehrich, D Epstein, HE Gould, WA Ims, RA Meltofte, H Raynolds, MK Sibik, J Talbot, SS Webber, PJ AF Walker, D. A. Daniels, F. J. A. Alsos, I. Bhatt, U. S. Breen, A. L. Buchhorn, M. Bueltmann, H. Druckenmiller, L. A. Edwards, M. E. Ehrich, D. Epstein, H. E. Gould, W. A. Ims, R. A. Meltofte, H. Raynolds, M. K. Sibik, J. Talbot, S. S. Webber, P. J. TI Circumpolar Arctic vegetation: a hierarchic review and roadmap toward an internationally consistent approach to survey, archive and classify tundra plot data SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Review DE climate change; remote sensing; vegetation classification; Arctic; biodiversity; circumpolar ID LAND-COVER MAP; PLANT-COMMUNITIES; BIOCLIMATE GRADIENT; GLACIAL SURVIVAL; SHRUB EXPANSION; NORTHERN ALASKA; CLIMATE-CHANGE; CLASSIFICATION; PATTERNS; RIVER AB Satellite-derived remote-sensing products are providing a modern circumpolar perspective of Arctic vegetation and its changes, but this new view is dependent on a long heritage of ground-based observations in the Arctic. Several products of the Conservation of Arctic Flora and Fauna are key to our current understanding. We review aspects of the PanArctic Flora, the Circumpolar Arctic Vegetation Map, the Arctic Biodiversity Assessment, and the Arctic Vegetation Archive (AVA) as they relate to efforts to describe and map the vegetation, plant biomass, and biodiversity of the Arctic at circumpolar, regional, landscape and plot scales. Cornerstones for all these tools are ground-based plant-species and plant-community surveys. The AVA is in progress and will store plot-based vegetation observations in a public-accessible database for vegetation classification, modeling, diversity studies, and other applications. We present the current status of the Alaska Arctic Vegetation Archive (AVA-AK), as a regional example for the panarctic archive, and with a roadmap for a coordinated international approach to survey, archive and classify Arctic vegetation. We note the need for more consistent standards of plot-based observations, and make several recommendations to improve the linkage between plot-based observations biodiversity studies and satellite-based observations of Arctic vegetation. C1 [Walker, D. A.; Buchhorn, M.; Druckenmiller, L. A.; Raynolds, M. K.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Daniels, F. J. A.; Bueltmann, H.] Univ Munster, Inst Biol & Biotechnol Plants, Munster, Germany. [Alsos, I.] UiT, Tromso Museum, Tromso, Norway. [Bhatt, U. S.] Univ Alaska Fairbanks, Dept Amospher Sci, Fairbanks, AK 99775 USA. [Breen, A. L.] Univ Alaska Fairbanks, Int Arctic Res Ctr, Fairbanks, AK 99775 USA. [Edwards, M. E.] Univ Southampton, Dept Geog & Environm, Southampton SO9 5NH, Hants, England. [Ehrich, D.; Ims, R. A.] UiT, Dept Arctic & Marine Biol, Tromso, Norway. [Epstein, H. E.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22903 USA. [Gould, W. A.] US Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Meltofte, H.] Aarhus Univ, Dept Biosci, DK-8000 Aarhus C, Denmark. [Sibik, J.] Slovak Acad Sci, Bratislava, Slovakia. [Talbot, S. S.] US Fish & Wildlife Serv, Anchorage, AK USA. [Webber, P. J.] Michigan State Univ, Dept Plant Biol, Ann Arbor, MI USA. RP Walker, DA (reprint author), Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. EM dawalker@alaska.edu RI Ehrich, Dorothee/F-6492-2015 OI Ehrich, Dorothee/0000-0002-3028-9488 FU USA National Atmospheric and Space Administration Arctic Boreal Vulnerability Experiment (ABoVE) [NNX13AM20G]; NASA Land Cover and Land Use Change Program (LCLUC) [NNX14AD90G]; International Arctic Science Committee (IASC); Conservation of Arctic Flora and Fauna (CAFF) FX This paper evolved from a paper presented at the Arctic Biomass Workshop at the University of Alaska Fairbanks, 2-3 September 2014. Most of the research described here has been supported in part by the International Arctic Science Committee (IASC) and the Conservation of Arctic Flora and Fauna (CAFF). Primary funds for this article came from the USA National Atmospheric and Space Administration Arctic Boreal Vulnerability Experiment (ABoVE, Grant No. NNX13AM20G and the NASA Land Cover and Land Use Change Program (LCLUC Grant No. NNX14AD90G). NR 112 TC 3 Z9 3 U1 20 U2 29 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD MAY PY 2016 VL 11 IS 5 AR 055005 DI 10.1088/1748-9326/11/5/055005 PG 16 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DM6TC UT WOS:000376484300029 ER PT J AU Betti, M Bauwe, H Busch, FA Fernie, AR Keech, O Levey, M Ort, DR Parry, MAJ Sage, R Timm, S Walker, B Weber, APM AF Betti, Marco Bauwe, Hermann Busch, Florian A. Fernie, Alisdair R. Keech, Olivier Levey, Myles Ort, Donald R. Parry, Martin A. J. Sage, Rowan Timm, Stefan Walker, Berkley Weber, Andreas P. M. TI Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Review DE Crops; food production; genetic engineering; photorespiration; Rubisco; yield improvement ID CHLOROPLAST GLUTAMINE-SYNTHETASE; C-4 PHOTOSYNTHESIS; ARABIDOPSIS-THALIANA; NITRATE ASSIMILATION; TOBACCO PLANTS; GLYOXYLATE METABOLISM; GLYCINE DECARBOXYLASE; ANTISENSE REDUCTION; REDUCED ACTIVITIES; SOLANUM-TUBEROSUM AB Plant yield can be substantially improved by altering photorespiratory fluxes or engineering artificial bypasses to photorespiration, but under certain environmental or nutritional conditions, reduced photorespiration may be detrimental to plant performance.Recycling of the 2-phosphoglycolate generated by the oxygenase reaction of Rubisco requires a complex and energy-consuming set of reactions collectively known as the photorespiratory cycle. Several approaches aimed at reducing the rates of photorespiratory energy or carbon loss have been proposed, based either on screening for natural variation or by means of genetic engineering. Recent work indicates that plant yield can be substantially improved by the alteration of photorespiratory fluxes or by engineering artificial bypasses to photorespiration. However, there is also evidence indicating that, under certain environmental and/or nutritional conditions, reduced photorespiratory capacity may be detrimental to plant performance. Here we summarize recent advances obtained in photorespiratory engineering and discuss prospects for these advances to be transferred to major crops to help address the globally increasing demand for food and biomass production. C1 [Betti, Marco] Univ Seville, Fac Quim, Dept Bioquim Vegetal & Biol Mol, Seville 41012, Spain. [Bauwe, Hermann; Timm, Stefan] Univ Rostock, Dept Plant Physiol, D-18051 Rostock, Germany. [Busch, Florian A.] Australian Natl Univ, Res Sch Biol, GPO Box 4, Canberra, ACT 2601, Australia. [Fernie, Alisdair R.] Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany. [Keech, Olivier] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, S-90187 Umea, Sweden. [Levey, Myles] Univ Dusseldorf, Inst Plant Mol & Dev Biol, D-40225 Dusseldorf, Germany. [Ort, Donald R.; Walker, Berkley] USDA ARS, Global Change & Photosynthesis Res Unit, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Parry, Martin A. J.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Sage, Rowan] Univ Toronto, Dept Ecol & Evolutionary Biol, 25 Willcocks St, Toronto, ON M5S 3B2, Canada. [Walker, Berkley] Univ Illinois, Carl Woese Inst Genom Biol, Urbana, IL 61801 USA. [Weber, Andreas P. M.] Univ Dusseldorf, Cluster Excellence Plant Sci CEPLAS, Inst Plant Biochem, D-40225 Dusseldorf, Germany. RP Betti, M (reprint author), Univ Seville, Fac Quim, Dept Bioquim Vegetal & Biol Mol, Seville 41012, Spain. EM mbetti@us.es RI Weber, Andreas/A-6250-2011; Busch, Florian/I-3816-2012; Betti, Marco/E-6304-2010 OI Weber, Andreas/0000-0003-0970-4672; Busch, Florian/0000-0001-6912-0156; Betti, Marco/0000-0002-7334-5734 FU FEDER-Ministerio de Economia y Competitividad, Spain [AGL2014-54413-R] FX This article was conceived during the discussion session 'Round table on future avenues of photorespiration research: crop improvement' held at the meeting 'Photorespiration - Key to better crops' in Warnemunde in June 2015. This work was supported by FEDER-Ministerio de Economia y Competitividad, Spain, [project AGL2014-54413-R to M.B.]. NR 115 TC 5 Z9 5 U1 26 U2 46 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAY PY 2016 VL 67 IS 10 SI SI BP 2977 EP 2988 DI 10.1093/jxb/erw076 PG 12 WC Plant Sciences SC Plant Sciences GA DM9BH UT WOS:000376658300008 PM 26951371 ER PT J AU Hodges, M Dellero, Y Keech, O Betti, M Raghavendra, AS Sage, R Zhu, XG Allen, DK Weber, APM AF Hodges, Michael Dellero, Younes Keech, Olivier Betti, Marco Raghavendra, Agepati S. Sage, Rowan Zhu, Xin-Guang Allen, Doug K. Weber, Andreas P. M. TI Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Review DE Biotic and abiotic stress; flux modeling; metabolic network; metabolic repair; photorespiration; regulation ID PEROXISOMAL HYDROXYPYRUVATE REDUCTASE; ARABIDOPSIS FOLLOWING CHANGES; GLYCINE DECARBOXYLASE; RESPIRATORY METABOLISM; CARBOXYLASE-OXYGENASE; NITRATE ASSIMILATION; CARBON METABOLISM; LEAF PEROXISOMES; S-NITROSYLATION; ABIOTIC STRESS AB Recent advances in photorespiration research are described and future priorities to better understand the metabolic integration of the photorespiratory cycle within the complex network of plant primary metabolism are discussed.Photorespiration is an essential high flux metabolic pathway that is found in all oxygen-producing photosynthetic organisms. It is often viewed as a closed metabolic repair pathway that serves to detoxify 2-phosphoglycolic acid and to recycle carbon to fuel the Calvin-Benson cycle. However, this view is too simplistic since the photorespiratory cycle is known to interact with several primary metabolic pathways, including photosynthesis, nitrate assimilation, amino acid metabolism, C-1 metabolism and the Krebs (TCA) cycle. Here we will review recent advances in photorespiration research and discuss future priorities to better understand (i) the metabolic integration of the photorespiratory cycle within the complex network of plant primary metabolism and (ii) the importance of photorespiration in response to abiotic and biotic stresses. C1 [Hodges, Michael; Dellero, Younes] Univ Evry, Univ Paris 11, Inst Plant Sci Paris Saclay, CNRS,INRA, F-91405 Orsay, France. [Keech, Olivier] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, SE-90187 Umea, Sweden. [Betti, Marco] Univ Seville, Fac Quim, Dept Bioquim Vegetal & Biol Mol, E-41012 Seville, Spain. [Raghavendra, Agepati S.] Univ Hyderabad, Dept Plant Sci, Sch Life Sci, Hyderabad 500046, Andhra Pradesh, India. [Sage, Rowan] Univ Toronto, Dept Ecol & Evolutionary Biol, 25 Willcocks St, Toronto, ON M5S 3B2, Canada. [Zhu, Xin-Guang] Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Shanghai 200031, Peoples R China. [Allen, Doug K.] USDA ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Weber, Andreas P. M.] Univ Dusseldorf, Inst Plant Biochem, Cluster Excellence Plant Sci CEPLAS, Univ Str 1, D-40225 Dusseldorf, Germany. [Weber, Andreas P. M.] Cluster Excellence Plant Sci, D-40225 Dusseldorf, Germany. RP Hodges, M (reprint author), Univ Evry, Univ Paris 11, Inst Plant Sci Paris Saclay, CNRS,INRA, F-91405 Orsay, France. EM michael.hodges@u-psud.fr RI Weber, Andreas/A-6250-2011; Allen, Doug/M-2836-2013; Betti, Marco/E-6304-2010 OI Weber, Andreas/0000-0003-0970-4672; Allen, Doug/0000-0001-8599-8946; Betti, Marco/0000-0002-7334-5734 FU French State grant - IDEX Paris-Saclay [ANR-10-LABX-0040-SPS, ANR-11-IDEX-0003-02]; French Ministry of Higher Education and Research; JC Bose National Fellowship from Department of Science and Technology, New Delhi, India [SR/S2/JCB-06/2006]; Deutsche Forschungsgemeinschaft [WE 2231/8-2, IRTG 1525, EXC 1028] FX YD and MH were supported by a French State grant (ANR-10-LABX-0040-SPS) funded by the IDEX Paris-Saclay, ANR-11-IDEX-0003-02. YD was supported also by a PhD grant from the French Ministry of Higher Education and Research. Work in the laboratory of ASR is supported by a JC Bose National Fellowship Research Grant (no. SR/S2/JCB-06/2006) from the Department of Science and Technology, New Delhi, India. APMW appreciates support from the Deutsche Forschungsgemeinschaft, grants WE 2231/8-2, IRTG 1525, and EXC 1028. NR 111 TC 3 Z9 4 U1 15 U2 31 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD MAY PY 2016 VL 67 IS 10 SI SI BP 3015 EP 3026 DI 10.1093/jxb/erw145 PG 12 WC Plant Sciences SC Plant Sciences GA DM9BH UT WOS:000376658300011 PM 27053720 ER PT J AU Branham, SE Wright, SJ Reba, A Linder, CR AF Branham, Sandra E. Wright, Sara J. Reba, Aaron Linder, C. Randal TI Genome-Wide Association Study of Arabidopsis thaliana Identifies Determinants of Natural Variation in Seed Oil Composition SO JOURNAL OF HEREDITY LA English DT Article DE Arabidopsis thaliana; fatty acids; genome-wide association mapping; lipid metabolism; seed oil composition; triacylglycerol ID FATTY-ACID BIOSYNTHESIS; DIACYLGLYCEROL ACYLTRANSFERASE; TRIACYLGLYCEROL ACCUMULATION; OLEIC-ACID; GENE; THIOESTERASES; FEEDSTOCKS; EXPRESSION; PLANTS AB The renewable source of highly reduced carbon provided by plant triacylglycerols (TAGs) fills an ever increasing demand for food, biodiesel, and industrial chemicals. Each of these uses requires different compositions of fatty acid proportions in seed oils. Identifying the genes responsible for variation in seed oil composition in nature provides targets for bioengineering fatty acid proportions optimized for various industrial and nutrition goals. Here, we characterized the seed oil composition of 391 world-wide, wild accessions of Arabidopsis thaliana, and performed a genome-wide association study (GWAS) of the 9 major fatty acids in the seed oil and 4 composite measures of the fatty acids. Four to 19 regions of interest were associated with the seed oil composition traits. Thirty-four of the genes in these regions are involved in lipid metabolism or transport, with 14 specific to fatty acid synthesis or breakdown. Eight of the genes encode transcription factors. We have identified genes significantly associated with variation in fatty acid proportions that can be used as a resource across the Brassicaceae. Two-thirds of the regions identified contain candidate genes that have never been implicated in lipid metabolism and represent potential new targets for bioengineering. C1 [Branham, Sandra E.] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Wright, Sara J.] Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA. [Branham, Sandra E.; Reba, Aaron; Linder, C. Randal] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. RP Branham, SE (reprint author), ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA.; Branham, SE (reprint author), Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. EM sandra.branham@ars.usda.gov FU National Science Foundation [DEB-1011609] FX National Science Foundation doctoral dissertation improvement grant (DEB-1011609) to [SEB, CRL]. NR 44 TC 4 Z9 4 U1 4 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 EI 1465-7333 J9 J HERED JI J. Hered. PD MAY PY 2016 VL 107 IS 3 BP 248 EP 256 DI 10.1093/jhered/esv100 PG 9 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DM4DH UT WOS:000376295300006 PM 26704140 ER PT J AU Branham, SE Wright, SJ Reba, A Morrison, GD Linder, CR AF Branham, Sandra E. Wright, Sara J. Reba, Aaron Morrison, Ginnie D. Linder, C. Randal TI Genome-Wide Association Study in Arabidopsis thaliana of Natural Variation in Seed Oil Melting Point: A Widespread Adaptive Trait in Plants SO JOURNAL OF HEREDITY LA English DT Article DE adaptation; evolution; fatty acid composition; GWAS ID GENETIC-VARIATION; SUBSTRATE-SPECIFICITY; NATIVE RANGE; FATTY-ACIDS; MODEL; CLIMATE; LOCI; EVOLUTION; NETWORKS; GROWTH AB Seed oil melting point is an adaptive, quantitative trait determined by the relative proportions of the fatty acids that compose the oil. Micro- and macro-evolutionary evidence suggests selection has changed the melting point of seed oils to covary with germination temperatures because of a trade-off between total energy stores and the rate of energy acquisition during germination under competition. The seed oil compositions of 391 natural accessions of Arabidopsis thaliana, grown under common-garden conditions, were used to assess whether seed oil melting point within a species varied with germination temperature. In support of the adaptive explanation, long-term monthly spring and fall field temperatures of the accession collection sites significantly predicted their seed oil melting points. In addition, a genome-wide association study (GWAS) was performed to determine which genes were most likely responsible for the natural variation in seed oil melting point. The GWAS found a single highly significant association within the coding region of FAD2, which encodes a fatty acid desaturase central to the oil biosynthesis pathway. In a separate analysis of 15 a priori oil synthesis candidate genes, 2 (FAD2 and FATB) were located near significant SNPs associated with seed oil melting point. These results comport with others' molecular work showing that lines with alterations in these genes affect seed oil melting point as expected. Our results suggest natural selection has acted on a small number of loci to alter a quantitative trait in response to local environmental conditions. C1 [Branham, Sandra E.] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Wright, Sara J.] Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA. [Branham, Sandra E.; Reba, Aaron; Linder, C. Randal] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. [Morrison, Ginnie D.] Univ Missouri, Div Plant Sci, Columbia, MO USA. RP Branham, SE (reprint author), ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA.; Branham, SE (reprint author), Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. EM sandra.branham@ars.usda.gov FU National Science Foundation [DEB-1011609] FX This work was supported by a National Science Foundation doctoral dissertation improvement grant (DEB-1011609) to (S.E.P., C.R.L.). NR 53 TC 1 Z9 1 U1 5 U2 15 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 EI 1465-7333 J9 J HERED JI J. Hered. PD MAY PY 2016 VL 107 IS 3 BP 257 EP 265 DI 10.1093/jhered/esw008 PG 9 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DM4DH UT WOS:000376295300007 PM 26865732 ER PT J AU Hinze, LL Gazave, E Gore, MA Fang, DD Scheffler, BE Yu, JZ Jones, DC Frelichowski, J Percy, RG AF Hinze, Lori L. Gazave, Elodie Gore, Michael A. Fang, David D. Scheffler, Brian E. Yu, John Z. Jones, Don C. Frelichowski, James Percy, Richard G. TI Genetic Diversity of the Two Commercial Tetraploid Cotton Species in the Gossypium Diversity Reference Set SO JOURNAL OF HEREDITY LA English DT Article DE germplasm collection; Gossypium barbadense; Gossypium hirsutum; introgression; population structure ID GERMPLASM COLLECTION; UPLAND COTTON; HIRSUTUM L.; POPULATION-STRUCTURE; BARBADENSE; WILD; PATTERNS; ORIGIN; GENOME; DIVERSIFICATION AB A diversity reference set has been constructed for the Gossypium accessions in the US National Cotton Germplasm Collection to facilitate more extensive evaluation and utilization of accessions held in the Collection. A set of 105 mapped simple sequence repeat markers was used to study the allelic diversity of 1933 tetraploid Gossypium accessions representative of the range of diversity of the improved and wild accessions of G. hirsutum and G. barbadense. The reference set contained 410 G. barbadense accessions and 1523 G. hirsutum accessions. Observed numbers of polymorphic and private bands indicated a greater diversity in G. hirsutum as compared to G. barbadense as well as in wild-type accessions as compared to improved accessions in both species. The markers clearly differentiated the 2 species. Patterns of diversity within species were observed but not clearly delineated, with much overlap occurring between races and regions of origin for wild accessions and between historical and geographic breeding pools for cultivated accessions. Although the percentage of accessions showing introgression was higher among wild accessions than cultivars in both species, the average level of introgression within individual accessions, as indicated by species-specific bands, was much higher in wild accessions of G. hirsutum than in wild accessions of G. barbadense. The average level of introgression within individual accessions was higher in improved G. barbadense cultivars than in G. hirsutum cultivars. This molecular characterization reveals the levels and distributions of genetic diversity that will allow for better exploration and utilization of cotton genetic resources. C1 [Hinze, Lori L.; Yu, John Z.; Frelichowski, James; Percy, Richard G.] ARS, USDA, Southern Plains Agr Res Ctr, Crop Germplasm Res Unit, College Stn, TX USA. [Gazave, Elodie; Gore, Michael A.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY USA. [Fang, David D.] ARS, USDA, So Reg Res Ctr, Cotton Fiber Biosci Res Unit, New Orleans, LA USA. [Scheffler, Brian E.] ARS, USDA, Jamie Whitten Delta States Res Ctr, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Jones, Don C.] Cotton Inc, Cary, NC USA. RP Hinze, LL (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Crop Germplasm Res Unit, College Stn, TX USA. EM Lori.Hinze@ars.usda.gov OI Hinze, Lori L/0000-0003-3356-1245; Scheffler, Brian/0000-0003-1968-8952 FU International Atomic Energy Agency Coordinated Research Project; Instituto Nacional de Tecnologia Agropecuaria-International Atomic Energy Agency Research [15671]; Agencia Nacional de Promocion Cientifica y Tecnologica Proyectos de Investigacion Cientifica y Tecnologica-Fondo para la Investigacion Cientifica y Tecnologica [620]; Instituto Nacional de Tecnologia Agropecuaria Proyecto Especifico Area Estrategica en Biotecnologia [244631] FX International Atomic Energy Agency Coordinated Research Project: Isolation and Characterization of Genes Involved in Mutagenesis of Plants, Instituto Nacional de Tecnologia Agropecuaria-International Atomic Energy Agency Research Contract No 15671: Isolation and Characterization of Genes Involved in Chloroplast Genes Mutagenesis, Agencia Nacional de Promocion Cientifica y Tecnologica Proyectos de Investigacion Cientifica y Tecnologica-Fondo para la Investigacion Cientifica y Tecnologica 2007 No 620: The barley chloroplast mutator as a tool to originate plastome genetic variability, and Instituto Nacional de Tecnologia Agropecuaria Proyecto Especifico Area Estrategica en Biotecnologia 244631: Mutagenesis techniques for diversity generation on characters of agricultural and/or agro-industrial interest. NR 46 TC 2 Z9 2 U1 1 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 EI 1465-7333 J9 J HERED JI J. Hered. PD MAY PY 2016 VL 107 IS 3 BP 274 EP 286 DI 10.1093/jhered/esw004 PG 13 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA DM4DH UT WOS:000376295300009 PM 26774060 ER PT J AU Temeyer, KB Tuckow, AP AF Temeyer, Kevin B. Tuckow, Alexander P. TI Tick Salivary Cholinesterase: A Probable Immunomodulator of Host-parasite Interactions SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE cholinesterase; acetylcholine; inflammation; parasite-host interaction; parasite immune regulation ID RHIPICEPHALUS BOOPHILUS MICROPLUS; PATHOGEN TRANSMISSION; ORGANOPHOSPHATE-RESISTANT; UP-REGULATION; WHOLE-BLOOD; ACETYLCHOLINESTERASE; MODULATION; EXPRESSION; IMMUNITY; IXODIDAE AB The southern cattle tick, Rhipicephalus (Boophilus) microplus (Canestrini), is the most economically important cattle ectoparasite in the world. Rhipicephalus microplus and Rhipicephalus annulatus (Say) continue to threaten U.S. cattle producers despite eradication and an importation barrier based on inspection, dipping of imported cattle in organophosphate (OP) acaricide, and quarantine of infested premises. OP acaricides inhibit acetylcholinesterase (AChE), essential to tick central nervous system function. Unlike vertebrates, ticks possess at least three genes encoding AChEs, differing in amino acid sequence and biochemical properties. Genomic analyses of R. microplus and the related tick, Ixodes scapularis, suggest that ticks contain many genes encoding different AChEs. This work is the first report of a salivary cholinesterase (ChE) activity in R. microplus, and discusses complexity of the cholinergic system in ticks and significance of tick salivary ChE at the tick-host interface. It further provides three hypotheses that the salivary ChE plausibly functions 1) to reduce presence of potentially toxic acetylcholine present in the large bloodmeal imbibed during rapid engorgement, 2) to modulate the immune response (innate and/or acquired) of the host to tick antigens, and 3) to influence transmission and establishment of pathogens within the host animal. Ticks are vectors for a greater number and variety of pathogens than any other parasite, and are second only to mosquitoes (owing to malaria) as vectors of serious human disease. Saliva-assisted transmission (SAT) of pathogens is well-known; however, the salivary components participating in the SAT process remain to be elucidated. C1 [Temeyer, Kevin B.; Tuckow, Alexander P.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. RP Temeyer, KB (reprint author), USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM Kevin.Temeyer@ars.usda.gov; atuckow@gmail.com FU USDA (CRIS) [6205-32000-031-00D, 3094-32000-036-00]; USDA, ARS, Postdoctoral Research Program award FX We thank Kristie Schlechte, Dave Krska, and Jason Tidwell for technical support and help with tick dissection. USDA is an equal opportunity provider and employer. This work was funded by USDA appropriated funds (CRIS # 6205-32000-031-00D and 3094-32000-036-00). A.P. Tuckow was supported by a USDA, ARS, Postdoctoral Research Program award to K.B. Temeyer. NR 46 TC 0 Z9 0 U1 0 U2 2 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD MAY PY 2016 VL 53 IS 3 BP 500 EP 504 DI 10.1093/jme/tjv252 PG 5 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA DM6II UT WOS:000376454800003 PM 26794231 ER PT J AU Kirn, DR Reid, KF Hau, C Phillips, EM Fielding, RA AF Kirn, Dylan R. Reid, Kieran F. Hau, Cynthia Phillips, Edward M. Fielding, Roger A. TI What is a Clinically Meaningful Improvement in Leg-Extensor Power for Mobility-limited Older Adults? SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE Physical performance; Muscle; Exercise; Power; Clinically important improvement ID EXTREMITY MUSCLE POWER; PHYSICAL PERFORMANCE; DISABILITY; STRENGTH; LIFE; TRIAL; MASS AB Background: Muscle power is a key predictor of physical function in older adults; however, clinically meaningful improvements in leg-extensor muscle power have yet to be identified. The purpose of this study is to establish the minimal clinically important improvement (MCII) and substantial improvement (SI) for leg-extensor power and muscle contraction velocity in mobility-limited older adults. Methods: Data were extracted from three randomized trials of leg-extensor muscle power training interventions (3- to 6-month duration). Measurements of leg-extensor power and velocity were obtained using dynamic bilateral leg press at 40% and 70% of the one-repetition maximum. Anchor-based MCIIs were calculated using selected items extracted from the Late Life Function and Disability Instrument. Standard error of measurement and effect size methods were used to calculate the distribution-based MCII. Results: Data from 164 participants (mean age: 76.6 +/- 5.6 years; Short Physical Performance Battery score: 7.8 +/- 1.3) were used in this analysis. The respective MCII and SI estimates for 40% leg-extensor power were 18.3 (9%) and 30.5 (15%) W, and 23.1 (10%) and 41.6 (18%) W for 70% leg-extensor power. The respective MCII and SI estimates for 40% average velocity were 0.03(7%) and 0.08(18%) m/s, and 0.02(6%) and 0.05(15%) m/s for 70% average velocity. Conclusions: This is the first study to establish a clinically meaningful improvement of leg-extensor power (9%-10%) and velocity (6%-7%) in mobility-limited older adults. These findings should be used to aid in the design and interpretation of clinical trials and interventions that target improvements in muscle power in this high-risk population. C1 [Kirn, Dylan R.; Reid, Kieran F.; Hau, Cynthia; Phillips, Edward M.; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St,Room 1316, Boston, MA 02111 USA. [Phillips, Edward M.] Harvard Univ, Sch Med, Dept Phys Med & Rehabil, Boston, MA USA. [Phillips, Edward M.] Joslin Diabet Ctr, Inst Lifestyle Med, Boston, MA 02215 USA. RP Fielding, RA (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St,Room 1316, Boston, MA 02111 USA. EM roger.fielding@tufts.edu FU U.S. Department of Agriculture [58-1950-4-003]; Boston Claude D. Pepper Older Americans Independence Center [1P30AG031679]; Boston Rehabilitation Outcomes Center Grant [1R24HD065688-01A1] FX This study was supported by the U.S. Department of Agriculture, under agreement No. 58-1950-4-003. This research was also supported by the Boston Claude D. Pepper Older Americans Independence Center (1P30AG031679) and the Boston Rehabilitation Outcomes Center Grant (1R24HD065688-01A1). NR 16 TC 2 Z9 2 U1 1 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD MAY PY 2016 VL 71 IS 5 BP 632 EP 636 DI 10.1093/gerona/glv207 PG 5 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DM5OE UT WOS:000376398400011 PM 26582075 ER PT J AU Groessl, EJ Kaplan, RM Sweet, CMC Church, T Espeland, MA Gill, TM Glynn, NW King, AC Kritchevsky, S Manini, T McDermott, MM Reid, KF Rushing, J Pahor, M AF Groessl, Erik J. Kaplan, Robert M. Sweet, Cynthia M. Castro Church, Timothy Espeland, Mark A. Gill, Thomas M. Glynn, Nancy W. King, Abby C. Kritchevsky, Stephen Manini, Todd McDermott, Mary M. Reid, Kieran F. Rushing, Julia Pahor, Marco CA LIFE Study Grp TI Cost-effectiveness of the LIFE Physical Activity Intervention for Older Adults at Increased Risk for Mobility Disability SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Article DE Cost-effectiveness; Physical activity; Older adults ID STYLE INTERVENTIONS; COMMUNITY; INDEPENDENCE; PREVENTION; EXERCISE; QUALITY; ELDERS; DESIGN; TRIAL AB Background: Losing the ability to walk safely and independently is a major concern for many older adults. The Lifestyle Interventions and Independence for Elders study recently demonstrated that a physical activity (PA) intervention can delay the onset of major mobility disability. Our objective is to examine the resources required to deliver the PA intervention and calculate the incremental cost-effectiveness compared with a health education intervention. Methods: The Lifestyle Interventions and Independence for Elders study enrolled 1,635 older adults at risk for mobility disability. They were recruited at eight field centers and randomly assigned to either PA or health education. The PA program consisted of 50-minute center-based exercise 2x weekly, augmented with home-based activity to achieve a goal of 150 min/wk of PA. Health education consisted of weekly workshops for 26 weeks, and monthly sessions thereafter. Analyses were conducted from a health system perspective, with a 2.6-year time horizon. Results: The average cost per participant over 2.6 years was US$3,302 and US$1,001 for the PA and health education interventions, respectively. PA participants accrued 0.047 per person more Quality-Adjusted Life-Years (QALYs) than health education participants. PA interventions costs were slightly higher than other recent PA interventions. The incremental cost-effectiveness ratios were US$42,376/major mobility disability prevented and US$49,167/QALY. Sensitivity analyses indicated that results were relatively robust to varied assumptions. Conclusions: The PA intervention costs and QALYs gained are comparable to those found in other studies. The ICERS are less than many commonly recommended medical treatments. Implementing the intervention in non-research settings may reduce costs further. C1 [Groessl, Erik J.] Univ Calif San Diego, Dept Family Med & Publ Hlth, La Jolla, CA 92093 USA. [Groessl, Erik J.] VA San Diego Healthcare Syst, San Diego, CA USA. [Kaplan, Robert M.] Agcy Healthcare Res & Qual, Rockville, MD USA. [Sweet, Cynthia M. Castro] Stanford Univ, Sch Med, Dept Med, Stanford Prevent Res Ctr, Stanford, CA 94305 USA. [Church, Timothy] Louisiana State Univ Syst, Pennington Biomed Res Ctr, Baton Rouge, LA USA. [Espeland, Mark A.; Rushing, Julia] Wake Forest Sch Med, Dept Biostat Sci, Winston Salem, NC USA. [Gill, Thomas M.] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA. [Glynn, Nancy W.] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Epidemiol, Pittsburgh, PA 15260 USA. [King, Abby C.] Stanford Univ, Sch Med, Dept Hlth Res & Policy & Med, Stanford, CA 94305 USA. [Kritchevsky, Stephen] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. [Manini, Todd] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL USA. [McDermott, Mary M.] Northwestern Univ, Dept Med & Prevent Med, Feinberg Sch Med, Chicago, IL 60611 USA. [Reid, Kieran F.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. RP Groessl, EJ (reprint author), Univ Calif San Diego, Dept Family Med & Publ Hlth, 9500 Gilman Dr,0994, San Diego, CA 92093 USA. EM egroessl@ucsd.edu OI Glynn, Nancy/0000-0003-2265-0162 FU National Institutes of Health/National Institute on Aging Cooperative Agreement [UO1 AG22376]; Intramural Research Program, National Institute on Aging [1I01CX000927-01A1]; National Institutes of Health; Claude D. Pepper Older Americans Independence Centers at the University of Florida [1 P30 AG028740]; Wake Forest University [1 P30 AG21332]; Tufts University [1P30AG031679]; University of Pittsburgh [P30 AG024827]; Yale University [P30AG021342]; National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Stanford University [UL1 RR025744]; Boston Rehabilitation Outcomes Center [1R24HD065688-01A1] FX The Lifestyle Interventions and Independence for Elders Study is funded by a National Institutes of Health/National Institute on Aging Cooperative Agreement (#UO1 AG22376) and a supplement from the National Heart, Lung and Blood Institute (3U01AG022376-05A2S), and sponsored in part by the Intramural Research Program, National Institute on Aging (No. 1I01CX000927-01A1) and National Institutes of Health. The research is partially supported by the Claude D. Pepper Older Americans Independence Centers at the University of Florida (1 P30 AG028740), Wake Forest University (1 P30 AG21332), Tufts University (1P30AG031679), University of Pittsburgh (P30 AG024827), and Yale University (P30AG021342); and the National Institutes of Health/National Center for Research Resources Clinical and Translational Science Awards at Stanford University (UL1 RR025744). Tufts University is also supported by the Boston Rehabilitation Outcomes Center (1R24HD065688-01A1). NR 31 TC 0 Z9 0 U1 3 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1079-5006 EI 1758-535X J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD MAY PY 2016 VL 71 IS 5 BP 656 EP 662 DI 10.1093/gerona/glw001 PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA DM5OE UT WOS:000376398400015 PM 26888433 ER PT J AU Jones, DR Guard, J Gast, RK Buhr, RJ Fedorka-Cray, PJ Abdo, Z Plumblee, JR Bourassa, DV Cox, NA Rigsby, LL Robison, CI Regmi, P Karcher, DM AF Jones, D. R. Guard, J. Gast, R. K. Buhr, R. J. Fedorka-Cray, P. J. Abdo, Z. Plumblee, J. R. Bourassa, D. V. Cox, N. A. Rigsby, L. L. Robison, C. I. Regmi, P. Karcher, D. M. TI Influence of commercial laying hen housing systems on the incidence and identification of Salmonella and Campylobacter SO POULTRY SCIENCE LA English DT Article DE hen housing systems; egg; Salmonella; Campylobacter; colonization ID EGG-PRODUCTION SYSTEMS; FURNISHED CAGES; INTERNAL ORGANS; ENVIRONMENTAL ASSESSMENT; ENRICHED CAGES; AIR-QUALITY; CONTAMINATION; ENTERITIDIS; HEALTH; IMPACT AB The housing of laying hens is important for social, industrial, and regulatory aspects. Many studies have compared hen housing systems on the research farm, but few have fully examined commercial housing systems and management strategies. The current study compared hens housed in commercial cagefree aviary, conventional cage, and enriched colony cage systems. Environmental and eggshell pool samples were collected from selected cages/segments of the housing systems throughout the production cycle and monitored for Salmonella and Campylobacter prevalence. At 77 wk of age, 120 hens per housing system were examined for Salmonella and Campylobacter colonization in the: adrenal glands, spleen, ceca, follicles, and upper reproductive tract. All isolates detected from environmental swabs, eggshell pools, and tissues were identified for serotype. Two predominant Salmonella were detected in all samples: S. Braenderup and S. Kentucky. Campylobacter coli and C. jejuni were the only Campylobacter detected in the flocks. Across all housing systems, approximately 7% of hens were colonized with Salmonella, whereas > 90% were colonized with Campylobacter. Salmonella Braenderup was the isolate most frequently detected in environmental swabs (P < 0.0001) and housing system impacted Salmonella spp. shedding (P < 0.0001). Campylobacter jejuni was the isolate most frequently found in environmental swabs (P < 0.01), while housing system impacted the prevalence of C. coli and jejuni in ceca (P < 0.0001). The results of this study provide a greater understanding of the impact of hen housing systems on hen health and product safety. Additionally, producers and academia can utilize the findings to make informed decisions on hen housing and management strategies to enhance hen health and food safety. C1 [Jones, D. R.; Guard, J.; Gast, R. K.; Buhr, R. J.; Fedorka-Cray, P. J.; Abdo, Z.; Plumblee, J. R.; Bourassa, D. V.; Cox, N. A.; Rigsby, L. L.] US Natl Poultry Res Ctr, USDA, ARS, Athens, GA 30605 USA. [Robison, C. I.; Regmi, P.; Karcher, D. M.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Fedorka-Cray, P. J.] N Carolina State Univ, Dept Populat Hlth & Pathobiol, Raleigh, NC 27695 USA. RP Jones, DR (reprint author), US Natl Poultry Res Ctr, USDA, ARS, Athens, GA 30605 USA. EM Deana.Jones@ars.usda.gov NR 42 TC 1 Z9 1 U1 4 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2016 VL 95 IS 5 BP 1116 EP 1124 DI 10.3382/ps/pew036 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DM4XX UT WOS:000376351700017 PM 26976901 ER PT J AU Bakst, MR Welch, GR Camp, MJ AF Bakst, M. R. Welch, G. R. Camp, M. J. TI Observations of turkey eggs stored up to 27 days and incubated for 8 days: embryo developmental stage and weight differences and the differentiation of fertilized from unfertilized germinal discs SO POULTRY SCIENCE LA English DT Article DE egg storage; turkeys; embryo development; parthenogenesis ID CHICK QUALITY; CELL-DEATH; STORAGE; HATCHABILITY; OVIPOSITION; BLASTODERM; IMPACT AB For logistical reasons, egg storage prior to incubation is a growing practice in the commercial turkey industry. Yet the consequence of increasing egg storage over 7 d is a progressive increase in embryo mortality. The objective of this study was to provide the information necessary to differentiate an early dead embryo from an unfertilized egg after 8 days of incubation (DOI). Five groups of eggs each from inseminated and virgin hens were stored for progressively increasing periods of time (5-d or less, 6 to 10 d, 11 to15 d, 16 to 20 d, and 21 to 27 d) and incubated. At 8 DOI, eggs were examined and the stage of development (Hamburger and Hamilton, 1951) and embryo weights in normally developed eggs were determined. There was a significant negative correlation between the stage of development and embryo weight with increasing storage periods. All remaining eggs from the inseminated and virgin hens were broken-out and the appearance of the yolk and the fertilized and unfertilized germinal discs examined. The yolks of both hen groups with unfertilized ova maintained a homogeneous uniform yellow-orange color. In contrast, yolks of ova that had been fertilized, with or without early-dead embryos, and yolks from virgin hens that showed evidence of parthenogenetic development (3%) had a heterogeneous appearance. Using fluorescence microscopy, the heterogeneous appearance was due to sheets of aberrant cells and less frequently dispersed cells and folds of the perivitelline layer. It was concluded that clear egg breakouts need to be performed to more accurately assess the impact of egg storage on embryonic mortality. Furthermore, such breakouts should be performed with a high intensity light directed across the surface of the germinal disc to clearly differentiate the subtle differences between an early-dead embryo and an unfertilized germinal disc. C1 [Bakst, M. R.; Welch, G. R.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD USA. [Camp, M. J.] ARS, Stat Grp, USDA, Beltsville, MD USA. RP Bakst, MR (reprint author), ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD USA. EM murray.bakst@ars.usda.gov NR 17 TC 0 Z9 0 U1 3 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY PY 2016 VL 95 IS 5 BP 1165 EP 1172 DI 10.3382/ps/pew010 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DM4XX UT WOS:000376351700024 PM 26957633 ER PT J AU Dangi, SR Zhang, HH Wang, D Gerik, J Hanson, BD AF Dangi, Sadikshya Rana Zhang, Huihui Wang, Dong Gerik, James Hanson, Bradley D. TI Soil Microbial Community Composition in a Peach Orchard Under Different Irrigation Methods and Postharvest Deficit Irrigation SO SOIL SCIENCE LA English DT Article DE Deficit irrigation; drip; furrow; microbial community biomass; microsprinkler ID WATER AVAILABILITY; DRIP IRRIGATION; TREES; PRODUCTIVITY; MANAGEMENT; PATTERNS; QUALITY; GROWTH; YIELD; FRUIT AB The San Joaquin Valley produces more than 250 unique crops and much of the U.S. fruits, vegetables, and nuts. One of the main limiting factors for production in this region is reduced availability of water. Deficit irrigation is a management practice where plants receive less than full irrigation but only at certain growth stages to minimize yield loss or long-term impacts to the crop. Given the multiple roles of soil microorganisms in the soil system, it is important to asses any impact of deficit irrigation on soil microbial communities. In the present study, full and deficit irrigation treatments were applied for 7 years using furrow, microsprinkler, and surface drip irrigation systems in a peach orchard. In the seventh year, soil samples were collected before and after implementation of deficit irrigation treatments to investigate effects on soil microbial community biomass and composition. Results showed that the proportion of Gram-positive bacteria and actinomycetes were significantly higher in furrow irrigation, whereas fungi and Gram-negative bacteria were lower in proportion under furrow irrigation as compared with microsprinkler and drip irrigation methods. Canonical variate analysis showed that soil microbial communities markedly differed after deficit treatment in furrow and microspray irrigation, but remained similar in surface drip irrigation in deficit and full irrigation. This may be due to frequent irrigation in surface drip where soil surface remained moist most of the time as compared with furrow and microsprinkler irrigation methods where irrigation was less frequent. C1 [Dangi, Sadikshya Rana; Zhang, Huihui; Wang, Dong; Gerik, James] ARS, Water Management Res Unit, San Joaquin Valley Agr Sci Ctr, USDA, 9611 S Riverbend Ave, Parlier, CA 93648 USA. [Dangi, Sadikshya Rana; Hanson, Bradley D.] Univ Calif Davis, Davis, CA 95616 USA. [Zhang, Huihui] ARS, Water Management Res, USDA, Ft Collins, CO USA. RP Dangi, SR (reprint author), ARS, Water Management Res Unit, San Joaquin Valley Agr Sci Ctr, USDA, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM Sadikshya.dangi@ars.usda.gov NR 39 TC 0 Z9 0 U1 8 U2 12 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD MAY PY 2016 VL 181 IS 5 BP 208 EP 215 DI 10.1097/SS.0000000000000153 PG 8 WC Soil Science SC Agriculture GA DM7ZJ UT WOS:000376579600003 ER PT J AU Gu, GH Mullen, CA Boateng, AA Vlachos, DG AF Gu, Geun Ho Mullen, Charles A. Boateng, Akwasi A. Vlachos, Dionisios G. TI Mechanism of Dehydration of Phenols on Noble Metals via First-Principles Microkinetic Modeling SO ACS CATALYSIS LA English DT Article DE phenols; microkinetic modeling; DFT; cresol; hydrodeoxygenation; dehydration; renewables; lignin ID CATALYTIC FAST PYROLYSIS; SUPPORTED PLATINUM CATALYSTS; FINDING SADDLE-POINTS; FREE-ENERGY RELATIONS; M-CRESOL; AQUEOUS-PHASE; BIO-OIL; HYDRODEOXYGENATION MECHANISM; FE CATALYSTS; LIGNIN MODEL AB Phenolic compounds constitute a sizable fraction of depolymerized biomass and are an ideal feedstock for the production of chemicals such as benzene and toluene. However, these compounds require catalytic upgrade via hydrodeoxygenation (HDO), a process whereby oxygen is removed as water by adding hydrogen while retaining the carbon molecular architecture. While the HDO of phenolics has been widely studied, a mechanism that is consistent with the data is still lacking. Herein, we perform first-principles microkinetic calculations for the HDO mechanism of an archetypical compound, p-cresol, on Pt(111). In contrast to the general belief, and in accordance with experimental data, we show that the single metal functionality is sufficient to carry out the HDO chemistry selectively, although ring activation is necessary. However, complete hydrogenation of the ring is neither necessary nor kinetically preferred. As a result, the conversion of p-cresol to toluene follows a complex energy landscape, where methylcyclohexanol and methylcyclohexane are not intermediates to toluene but rather share a common pool of intermediates with the hydrocarbons. C1 [Gu, Geun Ho; Vlachos, Dionisios G.] Univ Delaware, Catalysis Ctr Energy Innovat, Dept Biomol & Chem Engn, Newark, DE 19716 USA. [Gu, Geun Ho; Vlachos, Dionisios G.] Univ Delaware, Ctr Catalyt Sci & Technol, Newark, DE 19716 USA. [Mullen, Charles A.; Boateng, Akwasi A.] ARS, Sustainable Biofuels & Coprod, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Vlachos, DG (reprint author), Univ Delaware, Catalysis Ctr Energy Innovat, Dept Biomol & Chem Engn, Newark, DE 19716 USA.; Vlachos, DG (reprint author), Univ Delaware, Ctr Catalyt Sci & Technol, Newark, DE 19716 USA. EM vlachos@udel.edu FU USDA-NIFA-BRDI [2012-10008-20271]; USDA-ARS [59-1935-008]; National Science Foundation [OCI-1053575]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX This research was supported by a USDA-NIFA-BRDI grant (No. 2012-10008-20271) via an agreement with the USDA-ARS (No. 59-1935-008). The authors acknowledge computational resources of the Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by National Science Foundation Grant OCI-1053575. In connection with the XSEDE, the computations were performed on high-performance computing clusters administered by the Texas Advanced Computing Center (TACC) and on the Kraken cluster administered by the National Institute for Computational Sciences (NICS). This research also used resources of the National Energy Research Scientific Computing Center, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. G.H.G. is grateful to Dr. Matthew A. Christiansen, Dr. Jonathan Sutton, Vassili Vorotnikov, and Dr. Shengguang Wang for useful discussions. NR 81 TC 3 Z9 3 U1 24 U2 48 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2155-5435 J9 ACS CATAL JI ACS Catal. PD MAY PY 2016 VL 6 IS 5 BP 3047 EP 3055 DI 10.1021/acscatal.6b00776 PG 9 WC Chemistry, Physical SC Chemistry GA DL8LW UT WOS:000375894500036 ER PT J AU Millen, BE Abrams, S Adams-Campbell, L Anderson, CAM Brenna, JT Campbell, WW Clinton, S Hu, F Nelson, M Neuhouser, ML Perez-Escamilla, R Siega-Riz, AM Story, M Lichtenstein, AH AF Millen, Barbara E. Abrams, Steve Adams-Campbell, Lucile Anderson, Cheryl A. M. Brenna, J. Thomas Campbell, Wayne W. Clinton, Steven Hu, Frank Nelson, Miriam Neuhouser, Marian L. Perez-Escamilla, Rafael Siega-Riz, Anna Maria Story, Mary Lichtenstein, Alice H. TI The 2015 Dietary Guidelines Advisory Committee Scientific Report: Development and Major Conclusions SO ADVANCES IN NUTRITION LA English DT Review DE Dietary Guidelines; nutrition; conceptual model; physical activity; dietary patterns AB The Dietary Guidelines for Americans (DGA) is published every 5 y jointly by the Department of Health and Human Services (HHS) and the USDA and provides a framework for US-based food and nutrition programs, health promotion and disease prevention initiatives, and research priorities. Summarized in this report are the methods, major conclusions, and recommendations of the Scientific Report of the 2015 US Dietary Guidelines Advisory Committee (DGAC). Early in the process, the DGAC developed a conceptual model and formulated questions to examine nutritional risk and determinants and impact of dietary patterns in relation to numerous health outcomes among individuals aged >= 2 y. As detailed in the report, an expansive, transparent, and comprehensive process was used to address each question, with multiple opportunities for public input included. Consensus was reached on all DGAC's findings, including each conclusion and recommendation, and the entire report. When research questions were answered by original systematic literature reviews and/or with existing, high-quality expert reports, the quality and strength of the evidence was formally graded. The report was organized around the following 5 themes: 1) food and nutrient intakes and health: current status and trends; 2) dietary patterns, foods and nutrients, and health outcomes; 3) diet and physical activity behavior change; 4) food and physical activity environments; and 5) food sustainability and food safety. The following 3 cross-cutting topics were addressed: 1) sodium, 2) saturated fat, and 3) added sugars. Physical activity recommendations from recent expert reports were endorsed. The overall quality of the American diet was assessed to identify overconsumed and underconsumed nutrients of public health concern. Common food characteristics of healthy dietary patterns were determined. Features of effective interventions to change individual and population diet and physical activity behaviors in clinical, public health, and community settings were identified. The report was used by the HHS and the USDA to develop the 2015 DGA. C1 [Millen, Barbara E.] Millennium Prevent Inc, Westwood, MA USA. [Abrams, Steve] Univ Texas Houston, Dell Med Sch, Houston, TX USA. [Adams-Campbell, Lucile] Georgetown Univ, Med Ctr, Washington, DC 20007 USA. [Anderson, Cheryl A. M.] Univ Calif San Diego, Sch Med, San Diego, CA 92103 USA. [Brenna, J. Thomas] Cornell Univ, Ithaca, NY USA. [Campbell, Wayne W.] Purdue Univ, W Lafayette, IN 47907 USA. [Clinton, Steven] Ohio State Univ, Columbus, OH 43210 USA. [Hu, Frank] Harvard Univ, Sch Publ Hlth, 665 Huntington Ave, Boston, MA 02115 USA. [Nelson, Miriam] Tufts Univ, Medford, MA 02155 USA. [Neuhouser, Marian L.] Fred Hutchinson Canc Res Ctr, 1124 Columbia St, Seattle, WA 98104 USA. [Perez-Escamilla, Rafael] Yale Univ, Sch Publ Hlth, New Haven, CT USA. [Siega-Riz, Anna Maria] Gillings Sch Global Publ Hlth, Chapel Hill, NC USA. [Story, Mary] Duke Univ, Durham, NC USA. [Lichtenstein, Alice H.] Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM alice.lichtenstein@tufts.edu NR 6 TC 5 Z9 6 U1 16 U2 26 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 EI 2156-5376 J9 ADV NUTR JI Adv. Nutr. PD MAY PY 2016 VL 7 IS 3 BP 438 EP 444 DI 10.3945/an.116.012120 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DM2GB UT WOS:000376163900003 PM 27184271 ER PT J AU Millar, CI Westfall, RD Delany, DL AF Millar, Constance I. Westfall, Robert D. Delany, Diane L. TI Thermal components of American pika habitat-How does a small lagomorph encounter climate? SO ARCTIC ANTARCTIC AND ALPINE RESEARCH LA English DT Article ID OCHOTONA-PRINCEPS; SIERRA-NEVADA; GREAT-BASIN; POPULATION; DISPERSAL; PATTERNS; BEHAVIOR; USA AB Anticipating the response of small mammals to climate change requires knowledge of thermal conditions of their habitat during times of the day and year when individuals use them. We measured diurnal and seasonal temperatures of free air and of six habitat components for American pikas (Ochotona princeps) over five years at 37 sites in seven mountain ranges in the western Great Basin, United States. Talus matrices (subsurfaces) had low daily variances and, in the warm season, remained cool during the hottest times of the day relative to surfaces and free air. During winter, matrices were warmer than free air. Talus surfaces were warmer than free air in the warm and cold seasons, and had large daily variances. Summer forefield and dispersal environments were warmest of all habitat components. Talus surfaces in summer were highly responsive to solar radiation over the course of the day, warming quickly to high midday temperatures, and cooling rapidly in the evening. By contrast, matrices lagged the daily warm-up and remained warmer than free air at night. These differences afford diurnal and seasonal opportunities for pikas to adapt behaviorally to unfavorable temperatures and suggest that animals can accommodate a wider range of future climates than has been assumed, although warming of the dispersal environment may become limiting. Climate envelope models that use or model only surface air measures and do not include information on individual thermal components of pika habitat may lead to errant conclusions about the vulnerability of species under changing climates. C1 [Millar, Constance I.; Westfall, Robert D.; Delany, Diane L.] USDA, US Forest Serv, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. RP Millar, CI (reprint author), USDA, US Forest Serv, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. EM cmillar@fs.fed.us NR 35 TC 1 Z9 1 U1 24 U2 33 PU INST ARCTIC ALPINE RES PI BOULDER PA UNIV COLORADO, BOULDER, CO 80309 USA SN 1523-0430 EI 1938-4246 J9 ARCT ANTARCT ALP RES JI Arct. Antarct. Alp. Res. PD MAY PY 2016 VL 48 IS 2 BP 327 EP 343 DI 10.1657/AAAR0015-046 PG 17 WC Environmental Sciences; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA DM3DJ UT WOS:000376226700008 ER PT J AU Eskelson, BNI Monleon, VJ Fried, JS AF Eskelson, Bianca N. I. Monleon, Vicente J. Fried, Jeremy S. TI A 6 year longitudinal study of post-fire woody carbon dynamics in California's forests SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE longitudinal analysis; post-fire dynamics; forest stand recovery; disturbance; Forest Inventory and Analysis ID UNITED-STATES; WILDFIRE; FIRE; CHRONOSEQUENCES; SUCCESSION; SEVERITY; CLIMATE; CO2 AB We examined the dynamics of aboveground forest woody carbon pools - live trees, standing dead trees, and down wood-during the first 6 years following wildfire across a wide range of conditions, which are characteristic of California forest fires. From repeated measurements of the same plots, we estimated change in woody carbon pools as a function of crown fire severity as indicated by a post-fire index, years since fire, pre-fire woody carbon, forest type group ( hardwood vs. softwood), elevation, and climate attributes. Our analysis relied on 130 U.S. national forest inventory plots measured before and 1 year after fire, with one additional remeasurement within 6 years after fire. There was no evidence of net change in total wood carbon, defined for this study as the wood in standing trees larger than 12.7 cm diameter at breast height and down wood larger than 7.6 cm in diameter, over the post-fire period in any of the three severity classes. Stands that burned at low severity exhibited considerable shifts from live to standing dead and down wood pools. In stands that burned at moderate severity, live wood decreased significantly whereas no net change was detected in standing dead or down wood. High severity fire burning resulted in movement from standing dead to down wood pools. Our results suggest that the carbon trajectories for stand-replacing fires may not be appropriate for the majority of California's forest area that burned at low to moderate severities. C1 [Eskelson, Bianca N. I.] Univ British Columbia, Dept Forest Resources Management, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada. [Monleon, Vicente J.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Fried, Jeremy S.] US Forest Serv, USDA, Pacific NW Res Stn, Portland Forestry Sci Lab, 620 SW Main St,Suite 400, Portland, OR 97205 USA. RP Eskelson, BNI (reprint author), Univ British Columbia, Dept Forest Resources Management, 2424 Main Mall, Vancouver, BC V6T 1Z4, Canada. EM bianca.eskelson@ubc.ca RI Eskelson, Bianca/P-6095-2016 OI Eskelson, Bianca/0000-0002-3398-2333 FU USDA Forest Service, PNW Research Station, Resource Monitoring and Assessment Program; USDA Forest Service Region 5; Western Wildlands Environmental Threat and Assessment Center; National Fire Plan FX This research was supported, in part, by funds provided by the USDA Forest Service, PNW Research Station, Resource Monitoring and Assessment Program; USDA Forest Service Region 5; the Western Wildlands Environmental Threat and Assessment Center; and the National Fire Plan. We appreciate review comments provided on an earlier draft by Theresa B. Jain, thank the associate editor and two anonymous reviewers for their valuable comments, Sara Loreno for map preparation, and Grace Carsky for editorial comments and proofreading. We also thank Michael Ryan for sharing Fig. 3 from Ryan et al. (2010) with us. NR 38 TC 2 Z9 2 U1 5 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2016 VL 46 IS 5 BP 610 EP 620 DI 10.1139/cjfr-2015-0375 PG 11 WC Forestry SC Forestry GA DL9EX UT WOS:000375945700002 ER PT J AU Alexander, LW Woeste, KE AF Alexander, Lisa W. Woeste, Keith E. TI Phenology, dichogamy, and floral synchronization in a northern red oak (Quercus rubra) seed orchard SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Quercus; seed orchard; genetic management; phenology ID POPULATIONS; TREES; TIME AB We developed a novel scoring system to assess spring phenology in a northern red oak (Quercus rubra L.) clonal seed orchard. The system was used to score from 304 to 364 ramets for three reproductive seasons and to place clones into early, intermediate, and late phenology classes. Although the absolute number of clones in each phenological class changed from year to year, the overall order of clonal flowering was highly stable (r(s) = 0.67, p < 0.001). Early-flowering clones flowered significantly longer than later flowering clones in all 3 years. Dichogamy was present in the orchard, with male flowers of a clone emerging from 1.4 to 3.0 d sooner than its female flowers. Mean dichogamy values for individual clones ranged from 0.0 to 4.9 (+/- 1.3) d. Year strongly influenced a clone's dichogamy value (F = 6.0, p = 0.004), whereas genotype had no influence. The mean overall phenological synchronicity for the 3 years of observations was 0.30 +/- 0.01 or about 30% overlap between the time when females were receptive and males were shedding pollen. This study represents the first effort to quantify phenology in an artificial population of northern red oak, and it provides a snapshot of the current relationship between temperature, phenology, and floral synchronization. C1 [Alexander, Lisa W.; Woeste, Keith E.] Purdue Univ, Dept Forestry & Nat Resources, 715 W State St,Pfendler Hall, W Lafayette, IN 47907 USA. [Woeste, Keith E.] Purdue Univ, USDA, US Forest Serv, Northern Res Stn,Hardwood Tree Improvement & Rege, 715 W State St,Pfendler Hall, W Lafayette, IN 47907 USA. [Alexander, Lisa W.] USDA ARS, US Natl Arboretum, 472 Cadillac Lane, McMinnville, TN 37110 USA. RP Alexander, LW (reprint author), Purdue Univ, Dept Forestry & Nat Resources, 715 W State St,Pfendler Hall, W Lafayette, IN 47907 USA.; Alexander, LW (reprint author), USDA ARS, US Natl Arboretum, 472 Cadillac Lane, McMinnville, TN 37110 USA. EM Lisa.Alexander@ars.usda.gov FU Hardwood Tree Improvement and Regeneration Center (USDA Forest Service, Northern Research Station); Department of Forestry and Natural Resources at Purdue University FX This work was support by the Hardwood Tree Improvement and Regeneration Center (USDA Forest Service, Northern Research Station) and the Department of Forestry and Natural Resources at Purdue University. James McKenna and Brian Beheler provided logistical and technical support for field data collection. The Indiana Department of Natural Resources graciously allowed access to seed orchard facilities. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that also may be suitable. NR 22 TC 1 Z9 1 U1 7 U2 11 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2016 VL 46 IS 5 BP 629 EP 636 DI 10.1139/cjfr-2015-0312 PG 8 WC Forestry SC Forestry GA DL9EX UT WOS:000375945700004 ER PT J AU Pszwaro, JL D'Amato, AW Burk, TE Russell, MB Palik, BJ Strong, TF AF Pszwaro, Justin L. D'Amato, Anthony W. Burk, Thomas E. Russell, Matthew B. Palik, Brian J. Strong, Terry F. TI Analysis of stand basal area development of thinned and unthinned Acer rubrum forests in the upper Great Lakes region, USA SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Acer rubrum; basal area growth; climate; Great Lakes region ID DIAMETER GROWTH; CLIMATE-CHANGE; DENSITY INDEX; UNITED-STATES; YIELD; MODEL; MANAGEMENT; INCREMENT AB Red maple (Acer rubrum L.), historically a common but not abundant tree species in North America, has increased in abundance throughout its range over the last several decades; however, it has received little attention in growth and yield studies. The objectives of this study were to (i) evaluate the effects of stocking level and stand density on overall patterns of red maple stand productivity and (ii) quantify these relationships across a wide range of stand age, site quality, geographic location, and climatic conditions. We used long-term measurements from 52 sites in Wisconsin and Michigan to examine growth responses of even-aged red maple stands to various levels of thinning. Using linear, mixed-effects modeling, future stand-level red maple basal area was modeled as a function of stand and plot characteristics and climatic variables. Growing season precipitation and its interaction with initial red maple basal area were significant predictors; however, they only collectively reduced the mean squared error by 2.1% relative to a base model containing solely stand and plot factors. Model projections indicated there was little difference in predicted future basal area for the range of climate conditions experienced by these stands highlighting red maple's wide tolerance of environmental conditions across the region. C1 [Pszwaro, Justin L.; D'Amato, Anthony W.; Burk, Thomas E.; Russell, Matthew B.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Palik, Brian J.] USDA, Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. [Strong, Terry F.] USDA, Forest Serv, No Res Stn, Rhinelander, WI 54501 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. RP Pszwaro, JL (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM pszwa001@umn.edu FU USDA Forest Service Northern Research Station; Minnesota Agricultural Experiment Station FX We thank C. Kern, D. Kastendick, and R. Cooke for assisting with various aspects of the study, as well as F. Falzone, K. Gill, N. Bolton, and M. Reinikainen for assisting with fieldwork. D. McKenney and P. Papadopol provided modeled climate data for the study site locations. Financial support for this research was provided by the USDA Forest Service Northern Research Station and the Minnesota Agricultural Experiment Station. NR 52 TC 0 Z9 0 U1 9 U2 13 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2016 VL 46 IS 5 BP 645 EP 655 DI 10.1139/cjfr-2015-0411 PG 11 WC Forestry SC Forestry GA DL9EX UT WOS:000375945700006 ER PT J AU Britton, JM DeRose, RJ Mock, KE Long, JN AF Britton, Justin M. DeRose, R. Justin Mock, Karen E. Long, James N. TI Herbivory and advance reproduction influence quaking aspen regeneration response to management in southern Utah, USA SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE browsing; coppice; herbivory; Populus tremuloides; silviculture ID POPULUS-TREMULOIDES; UNGULATE HERBIVORY; NORTH-AMERICA; BLACK-HILLS; FORESTS; COLORADO; ESTABLISHMENT; FIRE; RECRUITMENT; LANDSCAPE AB Recent concern regarding the potential decline of quaking aspen (Populus tremuloides Michx.) forests in the western United States has sparked concern over whether the species can be effectively regenerated. Using a retrospective approach, we quantified the response of regenerating aspen stems to an ordinary set of silvicultural treatments conducted over approximately the past decade in southern Utah, USA. A suite of variables describing stand structure and composition, stand vigor, physiographic factors, herbivore pressure, and treatment types were measured to predict the possible controls, as well as their relative importance, on aspen regeneration. Results suggested that aspen regeneration was most strongly related to browsing pressure, site preparation technique, and the presence of advance reproduction before treatment, which is a novel finding. Secondary predictors included elevation, site index, and overstory conditions, which are generally characteristics of stand vigor. Management recommendations based on our results should recognize the strong primary control that browsing pressure exerts on regeneration. First, the height of advance reproduction is inherently dependent on antecedent herbivory and also indicative of present browsing and should be assessed before treatment. Second, the most effective site preparation techniques, namely broadcast burning and browsing reduction, will directly reduce browsing pressure, assuming ungulate populations are not too large. Any management targeting timely and effective aspen regeneration should incorporate monitoring and (or) controlling browsing pressure, both before and after treatment. C1 [Britton, Justin M.; Mock, Karen E.; Long, James N.] Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. [Britton, Justin M.; Mock, Karen E.; Long, James N.] Utah State Univ, Ctr Ecol, 5230 Old Main Hill, Logan, UT 84322 USA. [DeRose, R. Justin] USDA, Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. RP DeRose, RJ (reprint author), USDA, Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. EM rjderose@fs.fed.us FU USU Cedar Mountain Initiative; USDA NIFA Food and Agricultural Sciences National Needs Graduate and Postgraduate Fellowship (NNF) Grants Program FX This work was funded by grants from the USU Cedar Mountain Initiative and USDA NIFA Food and Agricultural Sciences National Needs Graduate and Postgraduate Fellowship (NNF) Grants Program. Patrick Moore (USDA Forest Service) and Sarah Shaughnessy (Utah Division of Forestry, Fire and State Lands) served as extremely valuable resources, providing great assistance regarding sample site selection. NR 49 TC 0 Z9 0 U1 10 U2 10 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2016 VL 46 IS 5 BP 674 EP 682 DI 10.1139/cjfr-2016-0010 PG 9 WC Forestry SC Forestry GA DL9EX UT WOS:000375945700009 ER PT J AU Shettles, M Hilker, T Temesgen, H AF Shettles, Michael Hilker, Thomas Temesgen, Hailemariam TI Examination of uncertainty in per unit area estimates of aboveground biomass using terrestrial LiDAR and ground data SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE model error; sampling error; measurement error; Pacific Northwest ID NATIONAL FOREST INVENTORY; STANDING TREES; SCANNING LIDAR; LASER; VOLUME; AIRBORNE; RECONSTRUCTION; PREDICTION; CANOPY AB In estimating aboveground forest biomass (AGB), three sources of error that interact and propagate include (i) measurement error, the quality of the tree-level measurement data used as inputs for the individual-tree equations; (ii) model error, the uncertainty about the equations of the individual trees; and (iii) sampling error, the uncertainty due to having obtained a probabilistic or purposive sample, rather than a census, of the trees on a given area of forest land. Monte Carlo simulations were used to examine measurement, model, and sampling errors and to compare total uncertainty between models and between a phase-based terrestrial laser scanner (TLS) and traditional forest inventory instruments. Input variables for the equations were diameter at breast height, total tree height (defined the height from the uphill side of the tree to the tree top), and height to crown base; these were extracted from the terrestrial LiDAR data. Relative contributions for measurement, model, and sampling errors were 5%, 70%, and 25%, respectively, when using TLS, and 11%, 66%, and 23%, respectively, when using the traditional inventory measurements as inputs into the models. We conclude that the use of TLS can reduce measurement errors of AGB compared with traditional inventory measurements. C1 [Shettles, Michael] Forest Management Serv Ctr, USDA, US Forest Serv, 2150 Ctr Ave,Suite 341A, Ft Collins, CO 80526 USA. [Hilker, Thomas; Temesgen, Hailemariam] Oregon State Univ, 204 Peavy Hall, Corvallis, OR 97331 USA. [Hilker, Thomas] Univ Southampton, Dept Geog & Environm, Highfield Rd, Southampton SO17 1BJ, Hants, England. RP Hilker, T (reprint author), Oregon State Univ, 204 Peavy Hall, Corvallis, OR 97331 USA.; Hilker, T (reprint author), Univ Southampton, Dept Geog & Environm, Highfield Rd, Southampton SO17 1BJ, Hants, England. EM T.Hilker@soton.ac.uk NR 36 TC 1 Z9 1 U1 6 U2 14 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2016 VL 46 IS 5 BP 706 EP 715 DI 10.1139/cjfr-2015-0265 PG 10 WC Forestry SC Forestry GA DL9EX UT WOS:000375945700012 ER PT J AU Levine, CR Krivak-Tetley, F van Doorn, NS Ansley, JAS Battles, JJ AF Levine, Carrie R. Krivak-Tetley, Flora van Doorn, Natalie S. Ansley, Jolie-Anne S. Battles, John J. TI Long-term demographic trends in a fire suppressed mixed-conifer forest SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE California; mixed conifer; fire; suppression; climate change; Bayesian ID TREE MORTALITY-RATES; OLD-GROWTH FORESTS; SIERRA-NEVADA; CALIFORNIA; PINE; PRECIPITATION; TEMPERATE; PATTERNS; INCREASE; REGIMES AB In the western United States, forests are experiencing novel environmental conditions related to a changing climate and a suppression of the historical fire regime. Mixed-conifer forests, considered resilient to disturbance due to their heterogeneity in structure and composition, appear to be shifting to a more homogeneous state, but the timescale of these shifts is not well understood. Our objective was to assess the effects of climate and fire suppression on stand dynamics and demographic rates of an old-growth mixed-conifer forest in the Sierra Nevada. We used a Bayesian hierarchical analysis to quantify species and community rates of recruitment, growth, and mortality. Despite a warming climate, we found that stand density, basal area, and carbon have increased over 56 years. Fir recruitment and growth significantly exceeded the community-level median rates, whereas pine recruitment and growth was significantly lower than the community-level median rates. Shifts in species composition from a well-mixed stand to a more dense fir-dominated stand appear to be driven by low growth and recruitment rates of pines relative to firs. In forests such as these with consistent and relatively low mortality rates, we recommend that restoration and management activities be focused on promoting pine recruitment and growth. C1 [Levine, Carrie R.; Krivak-Tetley, Flora; van Doorn, Natalie S.; Ansley, Jolie-Anne S.; Battles, John J.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. [Krivak-Tetley, Flora] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA. [van Doorn, Natalie S.] USDA, Forest Serv, Pacific Southwest Res Stn, 800 Buchanan St, Albany, CA 94710 USA. [Ansley, Jolie-Anne S.] Duane Morris LLP, One Market Plaza,Suite 2200, San Francisco, CA 94105 USA. RP Levine, CR (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. EM crlevine@berkeley.edu RI Battles, John/G-8233-2012 OI Battles, John/0000-0001-7124-7893 NR 42 TC 0 Z9 0 U1 10 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD MAY PY 2016 VL 46 IS 5 BP 745 EP 752 DI 10.1139/cjfr-2015-0406 PG 8 WC Forestry SC Forestry GA DL9EX UT WOS:000375945700016 ER PT J AU Hirko, KA Spiegelman, D Barnett, JB Cho, E Willett, WC Hankinson, SE Eliassen, AH AF Hirko, Kelly A. Spiegelman, Donna Barnett, Junaidah B. Cho, Eunyoung Willett, Walter C. Hankinson, Susan E. Eliassen, A. Heather TI Dietary Patterns and Plasma Sex Hormones, Prolactin, and Sex Hormone-Binding Globulin in Premenopausal Women SO CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION LA English DT Article ID BREAST-CANCER RISK; SERUM ESTROGEN-LEVELS; FOOD FREQUENCY QUESTIONNAIRE; CORONARY-HEART-DISEASE; HEALTHY EATING INDEX; POSTMENOPAUSAL WOMEN; ALCOHOL-CONSUMPTION; MEDITERRANEAN DIET; REGRESSION-MODELS; STEROID-HORMONES AB Background: Sex hormones are important for breast cancer, but it is unclear whether dietary patterns influence hormone concentrations. Methods: Dietary pattern adherence scores for the alternate Mediterranean diet (aMED), Dietary Approaches to Stop Hypertension ( DASH), and Alternative Healthy Eating Index (AHEI) were calculated from semiquantitative food frequency questionnaires administered in 1995 and 1999. Premenopausal plasma concentrations of sex hormones were measured in samples collected in 1996 to 1999. We used generalized linear models to calculate geometric mean hormone concentrations across quartiles of dietary pattern scores among 1,990 women in the Nurses' Health Study II. Results: We did not observe significant associations between sex hormone concentrations and the DASH pattern and only one suggestive association between follicular estrone concentrations and the aMED pattern [top vs. bottom quartile -4.4%, 95% confidence interval (CI), -10.6% to 2.1%; P-trend = 0.06]. However, women in the top versus bottom quartile of AHEI score had lower concentrations of follicular (-9.1%; 95% CI, -16.1% to - 1.4%; P-trend - 0.04) and luteal (-7.5%; 95% CI, - 13.6% to -0.9%; P-trend - 0.01) estrone, luteal-free (-9.3%; 95% CI, - 16.8% to - 1.1%; P-trend - 0.01) and total (-6.7 %; 95% CI, - 14.3% to 1.5%; P-trend - 0.04) estradiol, follicular estradiol (-14.2%; 95% CI, -24.6% to -2.4%; P-trend - 0.05), and androstenedione (- 7.8%; 95% CI, - 15.4% to 0.4%; P-trend - 0.03). Conclusion: Diet quality measured by the AHEI is inversely associated with premenopausal estrogen concentrations. Given that we did not observe similar associations with the aMED or DASH patterns, our findings should be interpreted with caution. Impact: Given the role of estrogens in breast cancer etiology, our findings add to the substantial evidence on the benefits of adhering to a healthy diet. (C) 2016 AACR. C1 [Hirko, Kelly A.] Michigan State Univ, Dept Epidemiol & Biostat, E Lansing, MI 48824 USA. [Spiegelman, Donna; Willett, Walter C.; Hankinson, Susan E.; Eliassen, A. Heather] Harvard Univ, Brigham & Womens Hosp, Sch Med, Channing Div Network Med,Dept Med, Boston, MA 02115 USA. [Spiegelman, Donna; Willett, Walter C.; Hankinson, Susan E.; Eliassen, A. Heather] Harvard Univ, Dept Epidemiol, TH Chan Sch Publ Hlth, Boston, MA 02115 USA. [Spiegelman, Donna] Harvard Univ, Dept Biostat, TH Chan Sch Publ Hlth, Boston, MA 02115 USA. [Barnett, Junaidah B.; Willett, Walter C.] Harvard Univ, Dept Nutr, TH Chan Sch Publ Hlth, Boston, MA 02115 USA. [Barnett, Junaidah B.] Tufts Univ, Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. [Barnett, Junaidah B.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Cho, Eunyoung] Brown Univ, Warren Alpert Med Sch, Dept Dermatol, Providence, RI 02912 USA. [Cho, Eunyoung] Brown Univ, Sch Publ Hlth, Dept Epidemiol, Providence, RI 02912 USA. [Hankinson, Susan E.] Univ Massachusetts, Dept Biostat & Epidemiol, Amherst, MA 01003 USA. RP Hirko, KA (reprint author), Michigan State Univ, Dept Epidemiol & Biostat, Coll Human Med, 909 Fee Rd, E Lansing, MI 48824 USA. EM khirko@epi.msu.edu FU NIH [UM1 CA176726, R01 CA67262, 5R01 CA50385-18 Sup]; [R25 CA098566] FX This research was supported from the NIH UM1 CA176726 (to W.C. Willett) and R01 CA67262 (to S.E. Hankinson). K.A. Hirko is supported by the R25 CA098566 training grant. Additional funding was provided from the NIH 5R01 CA50385-18 Sup (to W.C. Willett and J.B. Barnett). NR 61 TC 0 Z9 0 U1 1 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1055-9965 EI 1538-7755 J9 CANCER EPIDEM BIOMAR JI Cancer Epidemiol. Biomarkers Prev. PD MAY PY 2016 VL 25 IS 5 BP 791 EP 798 DI 10.1158/1055-9965.EPI-15-1019 PG 8 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA DL7VG UT WOS:000375847600009 PM 26980437 ER PT J AU Song, MA Brasky, TM Marian, C Weng, DY Taslim, C Llanos, AA Dumitrescu, RG Liu, ZH Mason, JB Spear, SL Kallakury, BVS Freudenheim, JL Shields, PG AF Song, Min-Ae Brasky, Theodore M. Marian, Catalin Weng, Daniel Y. Taslim, Cenny Llanos, Adana A. Dumitrescu, Ramona G. Liu, Zhenhua Mason, Joel B. Spear, Scott L. Kallakury, Bhaskar V. S. Freudenheim, Jo L. Shields, Peter G. TI Genetic variation in one-carbon metabolism in relation to genome-wide DNA methylation in breast tissue from healthy women SO CARCINOGENESIS LA English DT Article ID QUANTITATIVE TRAIT LOCI; CANCER-RISK; 10-FORMYLTETRAHYDROFOLATE DEHYDROGENASE; POLYMORPHISMS; FOLATE; EXPRESSION; CELLS; METAANALYSIS; ASSOCIATION; POPULATION AB The findings in normal breast tissues from healthy women, before cancer has developed, indicates that genetic variants for one-carbon metabolism leading to DNA methylation alter the levels of DNA methylation, including methylation within their own genes.Single-nucleotide polymorphisms (SNPs) in one-carbon metabolism genes and lifestyle factors (alcohol drinking and breast folate) may be determinants of whole-genome methylation in the breast. DNA methylation profiling was performed using the Illumina Infinium HumanMethylation450 BeadChip in 81 normal breast tissues from women undergoing reduction mammoplasty and no history of cancer. ANCOVA, adjusting for age, race and body mass index, was used to identify differentially methylated (DM) CpGs. Gene expression, by the Affymetrix GeneChip Human Transcriptome Array 2.0, was correlated with DM. Biological networks of DM genes were assigned using Ingenuity Pathway Analysis. Fifty-seven CpG sites (CpGs) were DM in association with eight SNPs in FTHFD, MTHFD1, MTHFR, MTR, MTRR and TYMS (P < 5.0x10(-5)); 56% of the DM CpGs were associated with FTHFD SNPs, including DM within FTHFD. Gene expression was negatively correlated with FTHFD methylation (r = -0.25, P = 0.017). Four DM CpGs identified by SNPs in MTRR, MTHFR and FTHFD were significantly associated with alcohol consumption and/or breast folate. The top biological network of DM CpGs was associated with Energy Production, Molecular Transportation, and Nucleic Acid Metabolism. This is the first comprehensive study of the association between SNPs in one-carbon metabolism genes and genome-wide DNA methylation in normal breast tissues. These SNPs, especially FTHFD, as well as alcohol intake and folate exposure, appear to affect DM in breast tissues of healthy women. The finding that SNPs in FTHFD and MTR are associated with their own methylation is novel and highlights a role for these SNPs as methylation quantitative trait loci. C1 [Song, Min-Ae; Brasky, Theodore M.; Marian, Catalin; Weng, Daniel Y.; Taslim, Cenny; Shields, Peter G.] Ohio State Univ, Coll Med, Div Canc Prevent & Control, Columbus, OH 43210 USA. [Song, Min-Ae; Brasky, Theodore M.; Marian, Catalin; Weng, Daniel Y.; Taslim, Cenny; Shields, Peter G.] James Canc Hosp, Columbus, OH 43210 USA. [Marian, Catalin] Victor Babes Univ Med & Pharm, Biochem & Pharmacol Dept, Timisoara 300041, Romania. [Llanos, Adana A.] Rutgers Sch Publ Hlth, Dept Epidemiol, New Brunswick, NJ 08903 USA. [Llanos, Adana A.] Rutgers Canc Inst New Jersey, New Brunswick, NJ 08903 USA. [Dumitrescu, Ramona G.] Distilled Spirits Council United States, Washington, DC 20005 USA. [Liu, Zhenhua] Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Dept Nutr, Amherst, MA 01003 USA. [Mason, Joel B.] Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. [Spear, Scott L.] Georgetown Univ, Dept Plast Surg, Washington, DC 20057 USA. [Kallakury, Bhaskar V. S.] Georgetown Univ, Dept Pathol, Washington, DC 20057 USA. [Freudenheim, Jo L.] Univ Buffalo, Sch Publ Hlth & Hlth Profess, Dept Epidemiol & Environm Hlth, Buffalo, NY 14214 USA. RP Shields, PG (reprint author), Ohio State Univ, Ctr Comprehens Canc, Wexner Med Ctr, 460 W 10th Ave,9th Floor,Suite D920, Columbus, OH 43210 USA. EM peter.shields@osumc.edu FU Department of Defense, Alcohol Center of Excellence [BC022346]; Agricultural Research Service of the United States Department of Agriculture [58-1950-0-014] FX Department of Defense, Alcohol Center of Excellence (BC022346); Agricultural Research Service of the United States Department of Agriculture (Cooperative Agreement #58-1950-0-014). NR 50 TC 1 Z9 1 U1 6 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0143-3334 EI 1460-2180 J9 CARCINOGENESIS JI Carcinogenesis PD MAY PY 2016 VL 37 IS 5 BP 471 EP 480 DI 10.1093/carcin/bgw030 PG 10 WC Oncology SC Oncology GA DM1MH UT WOS:000376108900004 ER PT J AU Li, XH Feng, G Zhao, CY Shi, FZ AF Li, Xinhu Feng, Gary Zhao, Chengyi Shi, Fengzhi TI Characteristics of soil infiltration in the Tarim River floodplain SO ENVIRONMENTAL EARTH SCIENCES LA English DT Article DE Infiltration rate; River flooding; Topsoil; Tarim River; Hydraulic head ID LOWER REACHES; HYDRAULIC-PROPERTIES; RING-INFILTROMETERS; PONDING DEPTHS; CLIMATE-CHANGE; WATER; CHINA; BASIN; PLANT; SALINIZATION AB The ecology in the Tarim River Basin is influenced by inundation of its floodplain. To date, however, there is limited information about the infiltration properties of floodplain soils that are inundated by this river. The aim of this study was to investigate steady-infiltration rates of these soils under four different hydraulic heads (5, 10, 20, and 30 cm). Results showed that the mean infiltration rates were generally high and ranged from 3.17 to 3.50 cm h(-1) when the hydraulic head was between 5 and 30 cm. The steady infiltration increased slightly as the hydraulic head increased. The relationship between the hydraulic head and the infiltration rate can be described by the equation y = 0.0122 x + 3.072, (R-2 = 0.739), where y is the steady-infiltration rate and x is the hydraulic head. There was no significant difference between the infiltration rates for the four different hydraulic heads. The infiltration rate was highest for loamy sand, followed by sandy loam, and was lowest for silt soils. Infiltration was influenced by soil texture and crust, and was low (<1 cm h(-1), hydraulic head of 5 cm) for soils with thick crusts, and high for soils without surface crusts (mean of 6.09 cm h(-1), hydraulic head of 5 cm). The steady-infiltration rate was not influenced by soil organic matter. These results indicate that flood water can percolate into the floodplain soils and give an improved understanding of the factors that influence the ecological processes on the Tarim River floodplains. C1 [Li, Xinhu; Zhao, Chengyi; Shi, Fengzhi] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Xinjiang, Peoples R China. [Feng, Gary] USDA ARS, Mississippi State, MS 39762 USA. RP Li, XH (reprint author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi 830011, Xinjiang, Peoples R China. EM lixinhu@ms.xjb.ac.cn FU National Natural Science Foundation of China [41201040, 41571035, 41371011]; National 973 project of China [2013CB429905]; Foundation of Chinese Academy of Sciences [XBBS201207] FX This research was supported by the National Natural Science Foundation of China (Grant No. 41201040, 41571035 and 41371011), the National 973 project (No. 2013CB429905) of China, and the Foundation of Chinese Academy of Sciences (Grant No. XBBS201207). NR 57 TC 0 Z9 0 U1 2 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1866-6280 EI 1866-6299 J9 ENVIRON EARTH SCI JI Environ. Earth Sci. PD MAY PY 2016 VL 75 IS 9 AR 782 DI 10.1007/s12665-016-5573-x PG 7 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA DM0JK UT WOS:000376030100059 ER PT J AU Riemann, R Liknes, G O'Neil-Dunne, J Toney, C Lister, T AF Riemann, Rachel Liknes, Greg O'Neil-Dunne, Jarlath Toney, Chris Lister, Tonya TI Comparative assessment of methods for estimating tree canopy cover across a rural-to-urban gradient in the mid-Atlantic region of the USA SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Tree canopy cover; Object-based image analysis; Accuracy; Comparative assessment; Forest inventory ID FOREST INVENTORY AB Tree canopy cover significantly affects human and wildlife habitats, local hydrology, carbon cycles, fire behavior, and ecosystem services of all types. In addition, changes in tree canopy cover are both indicators and consequences of a wide variety of disturbances from urban development to climate change. There is growing demand for this information nationwide and across all land uses. The extensive inventory plot system managed by the USDA Forest Service Forest Inventory and Analysis (FIA) offers a unique opportunity for acquiring unbiased tree canopy cover information across broad areas. However, the estimates it produces had not yet been examined for comparative accuracy with other sources. In this study, we compared four different methods readily available and with significant potential for application over broad areas. The first two, field-collected and photointerpreted, are currently acquired by FIA on approximately 44,000 plots annually nationwide. The third method is a stem-mapping approach that models tree canopy cover from variables regularly measured on forested plots and is efficient enough to calculate nationwide. The fourth is a Geographic-Object-Based Image Analysis (GEOBIA) approach that uses both high-resolution imagery and leaf-off LiDAR data and has reported very high accuracies and spatial detail at state-wide levels of application. Differences in the spatial and temporal resolution and coverage of these four datasets suggest that they could provide complementary information if their relationships could be better understood. Plot-and county-level estimates of tree canopy cover derived from each of the four data sources were compared for 11 counties in Maryland, Pennsylvania, and West Virginia across a range of urbanization levels. We found high levels of systematic agreement between field and photointerpreted, stem-mapped and field, photointerpreted and GEOBIA estimates. In several cases, the relationship changed with the level of tree canopy cover. GEOBIA produced the highest tree cover estimates of all the methods compared. Results are discussed with respect to known differences between the methods and ground conditions found in both forest and nonforest areas. C1 [Riemann, Rachel] US Forest Serv, Forest Inventory & Anal, USDA, No Res Stn, 425 Jordan Rd, Troy, NY 12180 USA. [Liknes, Greg] US Forest Serv, Forest Inventory & Anal, USDA, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. [O'Neil-Dunne, Jarlath] Univ Vermont, Spatial Anal Lab, George D Aiken Ctr 205, Burlington, VT 05405 USA. [Toney, Chris] US Forest Serv, USDA, Rocky Mt Res Stn, 5775 US West Highway 10, Missoula, MT 59808 USA. [Lister, Tonya] US Forest Serv, Forest Inventory & Anal, USDA, No Res Stn, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. RP Riemann, R (reprint author), US Forest Serv, Forest Inventory & Anal, USDA, No Res Stn, 425 Jordan Rd, Troy, NY 12180 USA. EM rriemann@fs.fed.us NR 38 TC 0 Z9 0 U1 8 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD MAY PY 2016 VL 188 IS 5 AR 297 DI 10.1007/s10661-016-5281-8 PG 17 WC Environmental Sciences SC Environmental Sciences & Ecology GA DM0FC UT WOS:000376017400038 PM 27090528 ER PT J AU Mon, J Bronson, KF Hunsaker, DJ Thorp, KR White, JW French, AN AF Mon, Jarai Bronson, K. F. Hunsaker, D. J. Thorp, K. R. White, J. W. French, A. N. TI Interactive effects of nitrogen fertilization and irrigation on grain yield, canopy temperature, and nitrogen use efficiency in overhead sprinkler-irrigated durum wheat SO FIELD CROPS RESEARCH LA English DT Article DE Water-nitrogen interactions; Infrared thermometry; N use efficiency; Yellow berry ID MEDITERRANEAN ENVIRONMENT; CROP COEFFICIENTS; SOIL NITRATE; WATER-USE; INDEX; CULTIVARS; QUALITY; PROTEIN; BARLEY; ACCUMULATION AB Nitrogen and irrigation management are crucial in the production of high protein irrigated durum wheat (Triticum durum Desf.) in arid regions. However, as the availability of irrigation water decreases and potential costs and regulation of nitrogen (N) increase, there is a need to better understand how irrigation levels interacts with N fertilizer rates. A two-year field experiment was conducted in Maricopa, Arizona USA on a Casa Grande sandy loam to assess effects of N fertilizer and irrigation rates on grain yield, grain N, canopy temperatures yellow berry, and N use efficiency. Five rates of N fertilizer as urea ammonium nitrate (0, 84, 168, 252, and 336 kg N ha(-1)) were applied in three equal splits at Zadoks stages 30, 32, and 39. Ten un-randomized, sequential rates of irrigation ranging from 0.35 to 1.14 fraction of a non deficit base irrigation treatment (maintained >45% soil water depletion) were applied by sequentially varying the nozzles in a gradient in an overhead sprinkler system. Irrigation plus rain ranged from 230 to 660 mm in the first season, and 180 to 600 mm in the second season. Grain yield was maximum in 2013 at the 252 kg N ha(-1) fertilizer rate and at the 10th water level (1.14 irrigation), and between 168 kg and 252 kg N ha(-1) at the 8th water level (1.0 irrigation) in 2014. The maximum grain yield of 7500 kg ha(-1) in 2013 was reduced to 5000 kg ha(-1) in 2014 due to a warmer, shorter growing season. Economic optimum N rate was at water level 8 both years (196 and 138 kg N ha(-1) in 2013, and 2014, respectively). Recovery efficiency of added N was high in this system (i.e., >70%) at N fertilizer and water levels that maximized biomass and grain yields. Grain N was maximum at a lower water level (level 3 or 0.50-0.54 irrigation), was positively affected by N fertilizer rate, and was negatively related to yellow berry incidence. Canopy temperature minus air temperature values decreased linearly with increasing irrigation level. Nitrogen fertilizer applications reduced canopy temperature when water levels >0.54 and 0.69 irrigation fraction in 2013, and 2014, respectively. The study results suggested that canopy temperature and weather data that reflects the grain-filling period could be used to improve irrigation and N management, respectively. In short, irrigated durum wheat growers on this soil would achieve the economically optimum grain yield, with the least risk of yield or protein reduction, by applying 200 kg N ha(-1) at the base irrigation level which maintains root zone soil moisture depletion below 45%. Published by Elsevier B.V. C1 [Mon, Jarai; Bronson, K. F.; Hunsaker, D. J.; Thorp, K. R.; White, J. W.; French, A. N.] USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. RP Bronson, KF (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM kevin.bronson@ars.usda.gov NR 50 TC 0 Z9 0 U1 16 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 EI 1872-6852 J9 FIELD CROP RES JI Field Crop. Res. PD MAY PY 2016 VL 191 BP 54 EP 65 DI 10.1016/j.fcr.2016.02.011 PG 12 WC Agronomy SC Agriculture GA DM0RH UT WOS:000376053000006 ER PT J AU Gunther, NW Phillips, JG Sommers, C AF Gunther, Nereus W. Phillips, John G. Sommers, Christopher TI The Effects of 405-nm Visible Light on the Survival of Campylobacter on Chicken Skin and Stainless Steel SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID STAPHYLOCOCCUS-AUREUS MRSA; BLUE-LIGHT; NM LIGHT; POLYPHOSPHATE ADDITIVES; HIGH-INTENSITY; LISTERIA-MONOCYTOGENES; ESCHERICHIA-COLI; CONTACT SURFACES; IN-VITRO; JEJUNI AB Campylobacter spp. are foodborne pathogens responsible for a significant portion of human cases of bacterial-mediated gastrointestinal disease. A primary method for the introduction of Campylobacter into the food supply is through poultry products. Reducing the number of Campylobacter on poultry products may reduce the incidence of human disease. Research has been conducted on the use of light to inactivate Campylobacter on poultry products and processing environments. More recently, the use of high intensity visible 405-nm light has been proposed for the elimination of pathogenic bacteria. This study investigated the ability of 405-nm light to reduce Campylobacter jejuni and Campylobacter coli in poultry products. Campylobacter in chicken exudate were placed onto chicken skin or food-grade stainless steel before treatment with 405-nm light. A range of 405-nm light doses were applied to cocktails of six C. jejuni or six C. coli strains in exudate at 10 degrees C to minimize thermal effects. Little difference was observed between inactivation of C. jejuni and C. coli on poultry skin with only minor average reductions of 1.7 logs and 2.1 logs, respectively, at the maximal dose of 184-186J/cm(2). More noticeable differences were observed when the samples were placed on stainless steel and treated with a dose of 89J/cm(2), producing an average reduction of 3.0 logs for C. coli but only 1.1 logs for C. jejuni. The maximal dose (181-183J/cm(2)) applied to Campylobacter on stainless steel produced significant (p0.05) reductions for C. jejuni and C. coli of 4.9 logs and 5.1 logs, respectively. However, significant 405-nm-mediated reductions in Campylobacter numbers required exposure times to achieve necessary dose levels that might be impractical under processing conditions. In addition, the most potent exposure times likely produced secondary thermal effects by raising sample surface temperatures above 48 degrees C. C1 [Gunther, Nereus W.] USDA ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Phillips, John G.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Sommers, Christopher] USDA ARS, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Gunther, NW (reprint author), USDA ARS, Eastern Reg Res Ctr, Mol Characterizat Foodborne Pathogens Res Unit, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM jack.gunther@ars.usda.gov NR 33 TC 0 Z9 0 U1 4 U2 7 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAY PY 2016 VL 13 IS 5 BP 245 EP 250 DI 10.1089/fpd.2015.2084 PG 6 WC Food Science & Technology SC Food Science & Technology GA DM0CL UT WOS:000376010100003 PM 26938455 ER PT J AU Montesinos-Lopez, A Montesinos-Lopez, OA Crossa, J Burgueno, J Eskridge, KM Falconi-Castillo, E He, XY Singh, P Cichy, K AF Montesinos-Lopez, Abelardo Montesinos-Lopez, Osval A. Crossa, Jose Burgueno, Juan Eskridge, Kent M. Falconi-Castillo, Esteban He, Xinyao Singh, Pawan Cichy, Karen TI Genomic Bayesian Prediction Model for Count Data with Genotype x Environment Interaction SO G3-GENES GENOMES GENETICS LA English DT Article DE Bayesian model; count data; genome enabled prediction; Gibbs sampler; GenPred; shared data resource; genomic selection ID ENABLED PREDICTION; WIDE ASSOCIATION; PLANT; TRAITS AB Genomic tools allow the study of the whole genome, and facilitate the study of genotype-environment combinations and their relationship with phenotype. However, most genomic prediction models developed so far are appropriate for Gaussian phenotypes. For this reason, appropriate genomic prediction models are needed for count data, since the conventional regression models used on count data with a large sample size (n(T)) and a small number of parameters (p) cannot be used for genomic-enabled prediction where the number of parameters (p) is larger than the sample size (n(T)). Here, we propose a Bayesian mixed-negative binomial (BMNB) genomic regression model for counts that takes into account genotype by environment (GxE) interaction. We also provide all the full conditional distributions to implement a Gibbs sampler. We evaluated the proposed model using a simulated data set, and a real wheat data set from the International Maize and Wheat Improvement Center (CIMMYT) and collaborators. Results indicate that our BMNB model provides a viable option for analyzing count data. C1 [Montesinos-Lopez, Abelardo] CIMAT, Dept Estadist, Guanajuato 36240, Mexico. [Montesinos-Lopez, Osval A.; Crossa, Jose; Burgueno, Juan; He, Xinyao; Singh, Pawan] Int Maize & Wheat Improvement Ctr CIMMYT, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. [Eskridge, Kent M.] Univ Nebraska, Dept Stat, Lincoln, NE 68583 USA. [Falconi-Castillo, Esteban] INIAP, Panamer Sur Km 1, Quito, Ecuador. [Cichy, Karen] USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. RP Crossa, J (reprint author), Int Maize & Wheat Improvement Ctr CIMMYT, Apdo Postal 6-641, Mexico City 06600, DF, Mexico. EM j.crossa@cgiar.org NR 35 TC 0 Z9 0 U1 5 U2 8 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD MAY PY 2016 VL 6 IS 5 BP 1165 EP 1177 DI 10.1534/g3.116.028118 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA DM4CY UT WOS:000376294400003 PM 26921298 ER PT J AU Adomako-Ankomah, Y English, ED Danielson, JJ Pernas, LF Parker, ML Boulanger, MJ Dubey, JP Boyle, JP AF Adomako-Ankomah, Yaw English, Elizabeth D. Danielson, Jeffrey J. Pernas, Lena F. Parker, Michelle L. Boulanger, Martin J. Dubey, Jitender P. Boyle, Jon P. TI Host Mitochondrial Association Evolved in the Human Parasite Toxoplasma gondii via Neofunctionalization of a Gene Duplicate SO GENETICS LA English DT Article DE gene duplication; Toxoplasma gondii; Hammondia hammondi; Neospora caninum; neofunctionalization ID HAMMONDIA-HAMMONDI; NEOSPORA-CANINUM; PARASITOPHOROUS VACUOLE; PLASMODIUM-FALCIPARUM; DROSOPHILA-MELANOGASTER; ENDOPLASMIC-RETICULUM; FUNCTIONAL ORTHOLOGS; VIRULENCE; EVOLUTION; SELECTION AB In Toxoplasma gondii, an intracellular parasite of humans and other animals, host mitochondrial association (HMA) is driven by a gene family that encodes multiple mitochondrial association factor 1 (MAF1) proteins. However, the importance of MAF1 gene duplication in the evolution of HMA is not understood, nor is the impact of HMA on parasite biology. Here we used within- and between-species comparative analysis to determine that the MAF1 locus is duplicated in T. gondii and its nearest extant relative Hammondia hammondi, but not another close relative, Neospora caninum. Using cross-species complementation, we determined that the MAF1 locus harbors multiple distinct paralogs that differ in their ability to mediate HMA, and that only T. gondii and H. hammondi harbor HMA(+) paralogs. Additionally, we found that exogenous expression of an HMA(+) paralog in T. gondii strains that do not normally exhibit HMA provides a competitive advantage over their wild-type counterparts during a mouse infection. These data indicate that HMA likely evolved by neofunctionalization of a duplicate MAF1 copy in the common ancestor of T. gondii and H. hammondi, and that the neofunctionalized gene duplicate is selectively advantageous. C1 [Adomako-Ankomah, Yaw; English, Elizabeth D.; Danielson, Jeffrey J.; Boyle, Jon P.] Univ Pittsburgh, Kenneth P Dietrich Sch Arts & Sci, Dept Biol Sci, Pittsburgh, PA 15260 USA. [Pernas, Lena F.] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA. [Parker, Michelle L.; Boulanger, Martin J.] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC VP8 5C2, Canada. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Boyle, JP (reprint author), Univ Pittsburgh, Dept Biol Sci, LSA 101,4249 Fifth Ave, Pittsburgh, PA 15260 USA. EM boylej@pitt.edu FU Pew Scholarship in the Biomedical Sciences and National Institutes of Health (NIH) [AI114655]; NIH [AI73756] FX The authors thank Abby Primack for critical reading of the manuscript. This work was funded by a Pew Scholarship in the Biomedical Sciences and National Institutes of Health (NIH) grant AI114655 to J.P.B. NIH grant AI73756 (awarded to John C. Boothroyd, Stanford University) supported L.F.P. NR 56 TC 0 Z9 0 U1 3 U2 3 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD MAY PY 2016 VL 203 IS 1 BP 283 EP + DI 10.1534/genetics.115.186270 PG 28 WC Genetics & Heredity SC Genetics & Heredity GA DM0DD UT WOS:000376012100020 PM 26920761 ER PT J AU Du, H Chouvenc, T Osbrink, WLA Su, NY AF Du, H. Chouvenc, T. Osbrink, W. L. A. Su, N. -Y. TI Social interactions in the central nest of Coptotermes formosanus juvenile colonies SO INSECTES SOCIAUX LA English DT Article DE Trophallaxis; Proctodeal feeding; Stomodeal feeding; Wood consumption; Grooming; Brood care ID SUBTERRANEAN TERMITE ISOPTERA; DIVISION-OF-LABOR; AGE POLYETHISM; DEFENSE-MECHANISMS; TUNNEL EXCAVATION; RHINOTERMITIDAE; BEHAVIOR; ANT; FORMICIDAE; ETHOGRAM AB Juvenile colonies of Coptotermes formosanus Shiraki were investigated to determine the social interactions among all individuals near the central nest of a colony. The behavioral repertoire of whole colonies of subterranean termites has yet to be identified because of their cryptic nests. Colonies were placed in planar arenas, and their behavioral repertoire and activities were recorded with video cameras. All castes and larval instars were determined and behavioral interactions were monitored and described, including feeding behaviors, grooming, nest maintenance, and inactivity. When two termites interacted, it was also determined which one was the donor or the recipient of the act. An ethogram was constructed by calculating relative frequency of each behavior for first instar larvae, second instar larvae, first instar workers, second instar workers or older, queen, king, soldiers and presoldiers. Larval instars, primary reproductives and soldiers were mostly passive during interactions. Workers performed most of the tasks; however, there was a difference of task divisions among worker instars. Young workers (W (1)) performed most of the grooming of individuals in the central nest, while older workers (W (2+)) provided nest maintenance and sanitation, by collecting fecal matter from most nest mates. In addition, older workers were mostly in charge of caring for the primary reproductives and the maintenance of the royal chamber. This study identified instar-specific activities and provides a first insight into caste and age polyethism in C. formosanus. C1 [Du, H.; Chouvenc, T.; Su, N. -Y.] Univ Florida, Inst Food & Agr Sci, Ft Lauderdale Res & Educ Ctr, Dept Entomol & Nematol, 3205 Coll Ave, Ft Lauderdale, FL 33314 USA. [Osbrink, W. L. A.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78029 USA. RP Du, H (reprint author), Univ Florida, Inst Food & Agr Sci, Ft Lauderdale Res & Educ Ctr, Dept Entomol & Nematol, 3205 Coll Ave, Ft Lauderdale, FL 33314 USA. EM hdu@ufl.edu NR 61 TC 1 Z9 1 U1 7 U2 12 PU SPRINGER BASEL AG PI BASEL PA PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND SN 0020-1812 EI 1420-9098 J9 INSECT SOC JI Insect. Soc. PD MAY PY 2016 VL 63 IS 2 BP 279 EP 290 DI 10.1007/s00040-016-0464-4 PG 12 WC Entomology SC Entomology GA DM3PW UT WOS:000376259500010 ER PT J AU Carlson, T Barns, C Brownlie, D Cordell, K Dawson, C Koch, W Oye, G Ryan, C AF Carlson, Tom Barns, Chris Brownlie, David Cordell, Ken Dawson, Chad Koch, William Oye, Garry Ryan, Chris TI An Overview of America's National Wilderness Preservation System SO JOURNAL OF FORESTRY LA English DT Article C1 [Carlson, Tom] US Forest Serv, Arthur Carhart Natl Wilderness Training Ctr, USDA, Missoula, MT USA. [Barns, Chris] Bur Land Management, Arthur Carhart Natl Wilderness Training Ctr, Washington, DC USA. [Brownlie, David] US Fish & Wildlife Serv, Monomoy Natl Wildlife Refuge, Washington, DC USA. [Cordell, Ken] US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Missoula, MT USA. [Dawson, Chad] SUNY Coll Environm Sci & Forestry, Syracuse, NY USA. [Koch, William] US Fish & Wildlife Serv, Great Swamp Natl Wildlife Refuge, New York, NY USA. [Oye, Garry] Natl Pk Serv, Wilderness Stewardship & Recreat Management, New York, NY USA. [Ryan, Chris] US Forest Serv, Wilderness Rivers & Outfitters, USDA, Missoula, MT USA. RP Carlson, T (reprint author), US Forest Serv, Arthur Carhart Natl Wilderness Training Ctr, USDA, Missoula, MT USA. EM sws.tom@gmail.com NR 6 TC 1 Z9 1 U1 2 U2 2 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 289 EP 291 DI 10.5849/jof.15-064 PG 3 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500005 ER PT J AU Cordell, K Barns, C Brownlie, D Carlson, T Dawson, C Koch, W Oye, G Ryan, C AF Cordell, Ken Barns, Chris Brownlie, David Carlson, Tom Dawson, Chad Koch, William Oye, Garry Ryan, Chris TI Wilderness Stewardship in America Today and What We Can Do to Improve It SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Cordell, Ken] US Forest Serv, Aldo Leopold Inst Wilderness Res, USDA, Missoula, MT USA. [Barns, Chris] Arthur Carhart Natl Wilderness Training Ctr, Bur Land Management, New York, NY USA. [Carlson, Tom] US Forest Serv, Arthur Carhart Natl Wilderness Training Ctr, USDA, Missoula, MT USA. [Dawson, Chad] SUNY Coll Environm Sci & Forestry, Syracuse, NY USA. [Koch, William] US Fish & Wildlife Serv, Great Swamp Natl Wildlife Refuge, New York, NY USA. [Oye, Garry] Natl Pk Serv, New York, NY USA. [Ryan, Chris] US Forest Serv, USDA, Missoula, MT USA. RP Cordell, K (reprint author), US Forest Serv, Aldo Leopold Inst Wilderness Res, USDA, Missoula, MT USA. EM kencordell@gmail.com NR 11 TC 3 Z9 3 U1 1 U2 1 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 292 EP 297 DI 10.5849/jof.15-067 PG 6 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500006 ER PT J AU Dawson, CP Cordell, K Watson, AE Ghimire, R Green, GT AF Dawson, Chad P. Cordell, Ken Watson, Alan E. Ghimire, Ramesh Green, Gary T. TI The US Wilderness Managers Survey: Charting a Path for the Future SO JOURNAL OF FORESTRY LA English DT Article DE wilderness; stewardship; management ID ATTITUDES AB The Wilderness Manager Survey (WMS) was developed in 2014 to support interagency strategic planning for the National Wilderness Preservation System (NWPS) and asked managers about their perceived threats to the NWPS, the need for science information to support decisionmaking, the need for education and training, and the most important problems for managers in the future. The WMS was administered during Feb. 24 to May 19, 2014, to wilderness managers in the four federal agencies who manage the lands of the NWPS, and 368 wilderness managers responded. The important external and internal threats as perceived by managers for the NWPS for the next 20 years were the following: the lack of political and financial support for wilderness protection and management, invasive exotic plant or animal species, disconnected urban audiences unaware of wilderness, adjacent land management and incompatible uses, and legislation designating wilderness that included compromised natural conditions or incompatible special provisions for management. C1 [Dawson, Chad P.] SUNY Coll Environm Sci & Forestry, Fac Forest & Nat Resources Management, Syracuse, NY USA. [Cordell, Ken] Aldo Leopold Wilderness Res Inst, Missoula, MT 59801 USA. [Watson, Alan E.] US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Rocky Mt Station, Missoula, MT 59801 USA. [Ghimire, Ramesh; Green, Gary T.] Univ Georgia, Athens, GA 30602 USA. RP Dawson, CP (reprint author), SUNY Coll Environm Sci & Forestry, Fac Forest & Nat Resources Management, Syracuse, NY USA.; Cordell, K (reprint author), Aldo Leopold Wilderness Res Inst, Missoula, MT 59801 USA.; Watson, AE (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Rocky Mt Station, Missoula, MT 59801 USA.; Ghimire, R; Green, GT (reprint author), Univ Georgia, Athens, GA 30602 USA. EM cpdawson@esf.edu; kencordell@gmail.com; awatson@fs.fed.us; ghimire@uga.edu; ggreen@warnell.uga.edu NR 31 TC 3 Z9 3 U1 5 U2 5 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 298 EP 304 DI 10.5849/jof.15-007 PG 7 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500007 ER PT J AU Schwartz, MK Hahn, BA Hossack, BR AF Schwartz, Michael K. Hahn, Beth A. Hossack, Blake R. TI Where the Wild Things Are: A Research Agenda for Studying the Wildlife-Wilderness Relationship SO JOURNAL OF FORESTRY LA English DT Review DE ecoregions; National Wilderness Preservation System; stewardship; wildlife; wilderness; wilderness character ID BIGHORN SHEEP; UNITED-STATES; NONNATIVE FISH; CLIMATE-CHANGE; HABITAT; CONSEQUENCES; CONNECTIVITY; CONSERVATION; RECREATION; MANAGEMENT AB We explore the connection between US designated wilderness areas and wildlife with the goal of establishing a research agenda for better understanding this complex relationship. Our research agenda has two components. The first, "wildlife for wilderness," considers the impact of wildlife on wilderness character. Whereas studies show that wildlife is important in both the perception and actual enhancement of wilderness character, the context and particulars of this relationship' have not been evaluated. For instance, is knowing that a rare, native species is present in a wilderness area enough to increase perceptions of naturalness.(an important wilderness quality)? Or does the public need to observe the species or its sign (e.g., tracks) for this benefit? The second part of our research agenda, "wilderness for wildlife," considers the types of research needed to understand the impact of wilderness areas on wildlife and biodiversity conservation. Several studies show the effect of one area being designated wilderness on one wildlife species. Yet, there has been no research that examines how the networks of wilderness areas in the National Wilderness Preservation System (NWPS) are used by a species or a community of species. Furthermore, we found no studies that focused on how the NWPS affects ecological or trophic interactions among species. We hope that by providing a research agenda, we can spur multiple lines of research on the topic of wildlife and wilderness. C1 [Schwartz, Michael K.] US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, USDA, Missoula, MT USA. [Hahn, Beth A.; Hossack, Blake R.] Aldo Leopold Wilderness Res Inst, Missoula, MT 59801 USA. RP Schwartz, MK (reprint author), US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, USDA, Missoula, MT USA.; Hahn, BA; Hossack, BR (reprint author), Aldo Leopold Wilderness Res Inst, Missoula, MT 59801 USA. EM michaelkschwartz@fs.fed.us; beth_hahn@nps.gov; blake_hossack@usgs.gov NR 59 TC 2 Z9 2 U1 2 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 311 EP 319 DI 10.5849/jof.15-070 PG 9 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500009 ER PT J AU Holmes, TP Bowker, JM Englin, J Hjerpe, E Loomis, JB Phillips, S Richardson, R AF Holmes, Thomas P. Bowker, J. M. Englin, Jeffrey Hjerpe, Evan Loomis, John B. Phillips, Spencer Richardson, Robert TI A Synthesis of the Economic Values of Wilderness SO JOURNAL OF FORESTRY LA English DT Review DE recreation use value; passive use value; ecosystem services; economic impacts ID TRAVEL COST-ANALYSIS; CONTINGENT VALUATION; RECREATION; QUALITY; SURPLUS; DEMAND; OPTION; UTAH AB Early applications of wilderness economic research demonstrated that the values of natural amenities and commodities produced from natural areas could be measured in commensurate terms. To the surprise of many, the economic values of wilderness protection often exceeded the potential commercial values that might result from resource extraction. Here, the concepts and tools used in the economic analysis of wilderness are described, and the wilderness economic literature is reviewed with a focus on understanding trends in use, value, and economic impacts. Although our review suggests that each of these factors is trending upward, variations in research methods plus large gaps in the literature limit understanding of long-run trends. However, as new data on wilderness use, visitor origins, and spatially referenced features of landscapes are becoming increasingly available, more robust economic analysis of both onsite and offsite wilderness economic values and impacts is now becoming possible. C1 [Holmes, Thomas P.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC USA. [Bowker, J. M.] US Forest Serv, USDA, Res Triangle Pk, NC USA. [Englin, Jeffrey] Arizona State Univ, Tempe, AZ 85287 USA. [Hjerpe, Evan] Conservat Econ Inst, Madrid, Spain. [Loomis, John B.] Colorado State Univ, Ft Collins, CO 80523 USA. [Phillips, Spencer] Key Log Econ, Charlottesville, VA USA. [Richardson, Robert] Michigan State Univ, E Lansing, MI 48824 USA. RP Holmes, TP (reprint author), US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC USA.; Bowker, JM (reprint author), US Forest Serv, USDA, Res Triangle Pk, NC USA.; Englin, J (reprint author), Arizona State Univ, Tempe, AZ 85287 USA.; Hjerpe, E (reprint author), Conservat Econ Inst, Madrid, Spain.; Loomis, JB (reprint author), Colorado State Univ, Ft Collins, CO 80523 USA.; Phillips, S (reprint author), Key Log Econ, Charlottesville, VA USA.; Richardson, R (reprint author), Michigan State Univ, E Lansing, MI 48824 USA. EM tholmes@fs.fed.us; tui9@charter.net; jeffrey.englin@asu.edu; evan@conservationecon.org; john.loomis@colostate.edu; spencer@keylogeconomics.com; rbr@anr.msu.edu NR 57 TC 3 Z9 3 U1 1 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 320 EP 328 DI 10.5849/jof.14-136 PG 9 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500010 ER PT J AU Watson, AE Cordell, HK Manning, R Martin, S AF Watson, Alan E. Cordell, H. Ken Manning, Robert Martin, Steven TI The Evolution of Wilderness Social Science and Future Research to Protect Experiences, Resources, and Societal Benefits SO JOURNAL OF FORESTRY LA English DT Review DE recreation; climate change; restoration; ecosystem services ID RECREATION CONFLICT RESEARCH; WATERS CANOE AREA; VALUES CONFLICT; MANAGEMENT; ATTITUDES; FEES; PERCEPTIONS; INDICATORS; PERMITS; USERS AB The historic Wilderness Act celebrated its 50th anniversary in 2014, and wilderness social science shared a similar legacy. As paradoxical as it might seem, humans are an important part of wilderness, helping to define the very concept and representing an important component of wilderness use and management. Much of the past five decades of wilderness-related social science has focused on recreational use, documenting the impacts of recreation on wilderness resources and the quality of the wilderness experience, exploring application of the concept of recreational carrying capacity to wilderness, and developing planning and management frameworks for balancing the inherent tension between wilderness use and protecting the quality of wilderness resources and the experience of visiting wilderness. The Limits of Acceptable Change and related planning frameworks, including formulation of recreation-related indicators and standards, continues to help guide wilderness management today. Other programs of social science research have developed protocols for measuring and monitoring wilderness recreation, defined the root causes of conflict among wilderness users and identified management approaches to minimize this conflict, explored the appropriate and acceptable use of fees for wilderness use, and identified a growing suite of wilderness values. All of these programs of research and others that could not be included in this review article have helped guide wilderness management and policy. However, social science research has evolved as a function of changes in both wilderness and society. This evolution continues through a focus on public attitudes toward adaptation to climate change, public attitudes toward restoration in wilderness to correct past human intervention, appropriate use of technology in wilderness, and issues related to the relevance of wilderness in light of changes in society and use of public lands. This article tells the story of these changes in issues and the relationship between wilderness and the American people. C1 [Watson, Alan E.; Cordell, H. Ken] US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Manning, Robert] Univ Vermont, Burlington, VT 05405 USA. [Martin, Steven] Humboldt State Univ, Arcata, CA 95521 USA. RP Watson, AE; Cordell, HK (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Rocky Mt Res Stn, Missoula, MT USA.; Manning, R (reprint author), Univ Vermont, Burlington, VT 05405 USA.; Martin, S (reprint author), Humboldt State Univ, Arcata, CA 95521 USA. EM awatson@fs.fed.us; kencordell@gmail.com; robert.manning@uvm.edu; Steven.Martin@humboldt.edu NR 88 TC 2 Z9 2 U1 11 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 329 EP 338 DI 10.5849/jof.14-145 PG 10 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500011 ER PT J AU Cole, D AF Cole, David TI Boundary Waters Canoe Area Wilderness-A Long History of Management Guided by Science SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Cole, David] US Forest Serv, USDA, Aldo Leopold Wilderness Res Inst, Missoula, MT USA. RP Cole, D (reprint author), US Forest Serv, USDA, Aldo Leopold Wilderness Res Inst, Missoula, MT USA. EM dncole.work@gmail.com NR 17 TC 1 Z9 1 U1 1 U2 1 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 363 EP 364 DI 10.5849/jof.15-042 PG 2 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500014 ER PT J AU Naficy, CE Keeling, EG Landres, P Hessburg, PF Veblen, TT Sala, A AF Naficy, Cameron E. Keeling, Eric G. Landres, Peter Hessburg, Paul F. Veblen, Thomas T. Sala, Anna TI Wilderness in the 21 st Century: A Framework for Testing Assumptions about Ecological Intervention in Wilderness Using a Case Study of Fire Ecology in the Rocky Mountains SO JOURNAL OF FORESTRY LA English DT Review DE wilderness; restoration; intervention; fire ecology; untrammeled ID MIXED-CONIFER FORESTS; PONDEROSA PINE FORESTS; NORTH-CENTRAL IDAHO; SIERRA-NEVADA; SPATIAL-PATTERNS; UNITED-STATES; WILDLAND FIRES; NATIONAL-PARK; RESTORATION TREATMENTS; PACIFIC-NORTHWEST AB Changes in the climate and in key ecological processes are prompting increased debate about ecological restoration and other interventions in wilderness. The prospect of intervention in wilderness raises legal, scientific, and values-based questions about the appropriateness of possible actions. In this article, we focus on the role of science to elucidate the potential need for intervention. We review the meaning of "untrammeled" from the 1964 Wilderness Act to aid our understanding of the legal context for potential interventions in wilderness. We explore the tension between restraint and active intervention in managing wilderness and introduce a framework for testing ecological assumptions when evaluating restoration proposals. We illustrate use of the framework in the restoration of fire regimes and fuel conditions in ponderosa pine and mixed-conifer forests of the US Rocky Mountains. Even in this relatively well-studied example, we find that the assumptions underlying proposed interventions in wilderness need to be critically evaluated and tested before new, more intensive management paradigms are embraced. C1 [Naficy, Cameron E.; Veblen, Thomas T.] Univ Colorado, Dept Geog, Boulder, CO 80309 USA. [Keeling, Eric G.] SUNY Coll New Paltz, New Paltz, NY USA. [Landres, Peter] US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Missoula, MT USA. [Hessburg, Paul F.] US Forest Serv, Pacific NW Res Stn, USDA, Missoula, MT USA. [Sala, Anna] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Naficy, CE (reprint author), Univ Colorado, Dept Geog, Boulder, CO 80309 USA.; Landres, P (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, USDA, Missoula, MT USA. EM cameron.naficy@colorado.edu; plandres@fs.fed.us FU NSF Wildfire FIRE grant [0966472]; NSF Doctoral Dissertation Research Improvement grant [1302233]; Jerry O'Neal National Park Service Student Fellowship FX We thank three anonymous reviewers for their comments that greatly helped to clarify this article. This research was supported by the NSF Wildfire FIRE grant (award no. 0966472), an NSF Doctoral Dissertation Research Improvement grant (award no. 1302233), and the Jerry O'Neal National Park Service Student Fellowship. NR 139 TC 2 Z9 2 U1 3 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 384 EP 395 DI 10.5849/jof.15-010 PG 12 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500017 ER PT J AU Campbell, D Mutch, RW AF Campbell, Dave Mutch, Robert W. TI Time Shows the Wisdom of Letting Some Wilderness Fires Roam Freely SO JOURNAL OF FORESTRY LA English DT Editorial Material C1 [Campbell, Dave; Mutch, Robert W.] US Forest Serv, USDA, Hamilton, MT USA. RP Campbell, D; Mutch, RW (reprint author), US Forest Serv, USDA, Hamilton, MT USA. EM dave.campbell.ipfs@gmail.com; bobmutch@montana.com NR 1 TC 0 Z9 0 U1 0 U2 0 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 EI 1938-3746 J9 J FOREST JI J. For. PD MAY PY 2016 VL 114 IS 3 BP 418 EP 419 DI 10.5849/jof.15-122 PG 2 WC Forestry SC Forestry GA DM3BJ UT WOS:000376221500022 ER PT J AU Liu, J Li, YF Shi, HM Wang, T Wu, XL Sun, XJ Yu, LL AF Liu, Jie Li, Yanfang Shi, Haiming Wang, Thomas Wu, Xianli Sun, Xiangjun Yu, Liangli (Lucy) TI Components characterization of total tetraploid jiaogulan (Gynostemma pentaphyllum) saponin and its cholesterol-lowering properties SO JOURNAL OF FUNCTIONAL FOODS LA English DT Article DE Jiaogulan (Gynostemma pentaphyllum); Cholesterol-lowering effect; Saponin; Chemical structure; Bile acid; Cholesterol uptake ID PHASEOLUS-VULGARIS L.; PLASMA-CHOLESTEROL; DOWN-REGULATION; METABOLISM; ABSORPTION; MECHANISMS; HAMSTERS; RATS; MICE; ANTHOCYANINS AB This study characterized chemical structures of tetraploid jiaogulan saponins, and investigated their cholesterol-lowering effects and mechanisms in hamsters fed a high-fat diet (HFD). Nine saponins, including five reported for the first time, were obtained from total jiaogulan saponins (TJS) and elucidated for their chemical structures according to 2D NMR and HR-MS data. Administration of TJS effectively decreased plasma total cholesterol by 17.4% and 32.6%, triacylglycerols by 22.8% and 47.7% and LDL-C by 22.0% and 40.2%, respectively. Moreover, supplementation with TJS significantly increased faecal excretion of total cholesterol and bile acids by 43.8% and 64.4%, respectively. TJS significantly up-regulated intestinal mRNA levels of ABCG5/8, LXR alpha, hepatic mRNA CYP7A1 and ABCG5 in hamsters on HFD. In addition, TJS decreased cholesterol micelle solubility and suppressed cholesterol uptake in cultured Caco-2 cells. Taking together, TJS may reduce plasma cholesterol by increasing faecal cholesterol excretion and through modulation of genes involved in cholesterol absorption and metabolism. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Liu, Jie; Li, Yanfang; Shi, Haiming; Sun, Xiangjun; Yu, Liangli (Lucy)] Shanghai Jiao Tong Univ, Inst Food & Nutraceut Sci, Sch Agr & Biol, Shanghai 200240, Peoples R China. [Liu, Jie] Beijing Technol Business Univ BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China. [Wang, Thomas] USDA, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Wu, Xianli] ARS, Nutrients Data Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Yu, Liangli (Lucy)] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Sun, XJ (reprint author), Shanghai Jiao Tong Univ, Inst Food & Nutraceut Sci, Sch Agr & Biol, Shanghai 200240, Peoples R China.; Yu, LL (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM xjsun@sjtu.edu.cn; yu5@umd.edu FU special fund for Agroscientific Research in the Public Interest [201203069]; National High Technology Research and Development Program of China [2013AA102202, 2013AA102207]; Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.; Beijing Advanced Innovation Center for Food Nutrition and Human Health FX This work was partly supported by a special fund for Agroscientific Research in the Public Interest (Grant No. 201203069); National High Technology Research and Development Program of China (Grant Nos. 2013AA102202; 2013AA102207); a grant from Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd. and a grant from Beijing Advanced Innovation Center for Food Nutrition and Human Health. NR 34 TC 1 Z9 1 U1 9 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1756-4646 J9 J FUNCT FOODS JI J. Funct. Food. PD MAY PY 2016 VL 23 BP 542 EP 555 DI 10.1016/j.jff.2016.03.013 PG 14 WC Food Science & Technology SC Food Science & Technology GA DM1ZK UT WOS:000376145200051 ER PT J AU Claycombe, KJ Vomhof-DeKrey, EE Garcia, R Johnson, WT Uthus, E Roemmich, JN AF Claycombe, Kate J. Vomhof-DeKrey, Emilie E. Garcia, Rolando Johnson, William Thomas Uthus, Eric Roemmich, James N. TI Decreased beige adipocyte number and mitochondrial respiration coincide with increased histone methyl transferase (G9a) and reduced FGF21 gene expression in Sprague-Dawley rats fed prenatal low protein and postnatal high-fat diets SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article DE Beige adipocyte; FGF21; G9a; Mitochondrial respiration; Prenatal low-protein diet; Postnatal high-fat diet; Subcutaneous adipose tissue ID BROWN ADIPOSE-TISSUE; GROWTH-FACTOR 21; BODY-WEIGHT; STEM-CELLS; THERMOGENESIS; PRDM16; MICE; FIBROBLAST-GROWTH-FACTOR-21; INDUCTION; BALANCE AB We have shown that prenatal low-protein (LP) followed by postnatal high-fat (HF) diets result in a rapid increase in subcutaneous adipose tissue (subc-AT) mass in the offspring, contributing to development of obesity and insulin resistance. Studies have shown that a key transcription factor, PR domain containing 16 (PRDM16), and fibroblast growth factor 21 (FGF21) are involved in conversion of precursor cells into mitochondria (mt)-enriched beige adipocytes (BA). Our hypothesis is that a maternal LP and postnatal HF diets increase the risk of obesity and insulin resistance in offspring, in part, by reducing the conversion of precursor cell into BA in the subc-AT of offspring. Using obese-prone Sprague Dawley rats fed 8% LP or 20% normal-protein (NP) diets for 3 weeks prior to conception and throughout pregnancy and lactation followed by 12 weeks of 10% normal-fat (NF) or 45% HF diet feeding, we investigated whether prenatal LP and postnatal HF diets affect BA number and oxidative respiratory function in subc-AT. Results showed that subc-AT and liver FGF21, PRDM16 and BA marker CD137 mRNA increase with postnatal HF diet in maternal NP group rats. In contrast, rats fed maternal LP and postnatal HF diets showed no increase in subc-AT mt copy number, oxygen consumption rate, FGF21, PRDM16 and CD137 mRNA, whereas protein expression of an inhibitor for FGF21 transcription (histone methyltransferase, G9a) increased. These findings suggest that LPHF diets cause offspring metabolic alterations by reduced BA and FGF21 mRNA and increased G9a protein expression in subc-AT. Published by Elsevier Inc. C1 [Claycombe, Kate J.; Vomhof-DeKrey, Emilie E.; Garcia, Rolando; Johnson, William Thomas; Uthus, Eric; Roemmich, James N.] USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. RP Claycombe, KJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave N, Grand Forks, ND 58203 USA. EM kate.claycombe@ars.usda.gov FU USDA Agricultural Research Service Project [3062-51,000-052-00D] FX This work was supported by USDA Agricultural Research Service Project #3062-51,000-052-00D. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 29 TC 2 Z9 2 U1 4 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD MAY PY 2016 VL 31 BP 113 EP 121 DI 10.1016/j.jnutbio.2016.01.008 PG 9 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DL8KN UT WOS:000375891000013 PM 27133430 ER PT J AU Brown, JR Herrick, JE AF Brown, Joel R. Herrick, Jeffrey E. TI Making soil health a part of rangeland management SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article ID LAND MANAGEMENT; INDICATORS; RESILIENCE; QUALITY; DESERTIFICATION; SUSTAINABILITY; COMPONENT; ECOSYSTEM; SCIENCE C1 [Brown, Joel R.; Herrick, Jeffrey E.] USDA Nat Resources Conservat Serv, Jornada Expt Range, Washington, DC 20005 USA. RP Brown, JR (reprint author), USDA Nat Resources Conservat Serv, Jornada Expt Range, Washington, DC 20005 USA. NR 32 TC 1 Z9 1 U1 4 U2 9 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2016 VL 71 IS 3 BP 55A EP 60A DI 10.2489/jswc.71.3.55A PG 6 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DM3BC UT WOS:000376220800001 ER PT J AU Chambers, A Lal, R Paustian, K AF Chambers, Adam Lal, Rattan Paustian, Keith TI Soil carbon sequestration potential of US croplands and grasslands: Implementing the 4 per Thousand Initiative SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article ID ORGANIC-CARBON; AGRICULTURAL SOILS; CONSERVATION AGRICULTURE; NITROGEN-FERTILIZATION; MANAGEMENT IMPACTS; GAS EMISSIONS; COVER CROPS; MITIGATION; SWITCHGRASS; BIOENERGY C1 [Chambers, Adam] USDA Nat Resources Conservat Serv, West Natl Technol Support Ctr, Sci & Technol Deputy Area, Energy & Environm Markets Team, Portland, OR USA. [Lal, Rattan] Ohio State Univ, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. [Paustian, Keith] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Paustian, Keith] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Chambers, A (reprint author), USDA Nat Resources Conservat Serv, West Natl Technol Support Ctr, Sci & Technol Deputy Area, Energy & Environm Markets Team, Portland, OR USA. RI Paustian, Keith/L-7593-2016 NR 57 TC 3 Z9 3 U1 24 U2 29 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2016 VL 71 IS 3 BP 68A EP 74A DI 10.2489/jswc.71.3.68A PG 7 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DM3BC UT WOS:000376220800003 ER PT J AU Nouwakpo, SK Weitz, M Hernandez, M Champa, T Fisher, J AF Nouwakpo, S. K. Weitz, M. Hernandez, M. Champa, T. Fisher, J. TI Performance of the Rangeland Hydrology and Erosion Model for runoff and erosion assessment on a semiarid reclaimed construction site SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE construction site; rangeland erosion; rangeland hydrology; reclmation; RHEM; soil erosion ID SOIL LOSS EQUATION; BANDED VEGETATION PATTERNS; PER-STORM BASIS; SOUTHEASTERN ARIZONA; WESTERN RANGELANDS; SEAL FORMATION; CONSERVATION; CONNECTIVITY; THRESHOLDS; AUSTRALIA AB The ability to assess the impact of management actions on soil and water resources is crucial to sustainable land management. The Rangeland Hydrology and Erosion Model (RHEM) was developed as an assessment and decision support tool for resource management agencies and has been used to estimate soil erosion at national, regional, and local scales for both disturbed and undisturbed rangeland soil and vegetation conditions. In this paper, runoff, sediment, and microtopography data were collected during a series of rainfall simulation experiments aimed at evaluating the effectiveness of revegetation on a shrub-dominated post construction hillslope where the soil has had time to reconsolidate. RHEM's input parameters are simple to collect and consist of soil texture, slope configuration, and canopy and ground cover. Experimental results showed that a mix of the shrub species rabbitbrush (Chrysothamnus nauseosus) and the invasive annual grass cheatgrass (Bromus tectorum) were more effective at reducing runoff and soil loss than rabbitbrush alone. Plots with more than 45% of residue cover produced as much as 2.1 times less runoff and 16 times less sediments than those with less than 15% of litter. Microtopographic changes information allowed partitioning the erosion process into diffuse and concentrated flow processes. Analysis of this 3D data highlighted the central role of concentrated flow erosion in sediment delivery on rangeland hillslopes. RHEM's performance as expressed by the coefficient of determination, R-2, and the Nash-Stucliffe Efficiency (NSE) was R-2 = 0.84 and NSE = 0.27 for runoff and R-2 = 0.81 and NSE = 0.26 for sediments. This study demonsdates that RHEM can be effectively used by land managers and project managers to estimate soil erosion as a function of precipitation event on construction sites that have been revegetated to rangeland plant communities. C1 [Nouwakpo, S. K.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Weitz, M.] USDA ARS, Great Basin Rangeland Res Unit, Reno, NV USA. [Hernandez, M.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. [Champa, T.; Fisher, J.] USDA Nat Resources Conservat Serv, Reno, NV USA. RP Nouwakpo, SK (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. NR 75 TC 0 Z9 1 U1 8 U2 13 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2016 VL 71 IS 3 BP 220 EP 236 DI 10.2489/jswc.71.3.220 PG 17 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DM3BC UT WOS:000376220800009 ER PT J AU Merten, GH Welch, HL Tomer, MD AF Merten, G. H. Welch, H. L. Tomer, M. D. TI Y Effects of hydrology, watershed size, and agricultural practices on sediment yields in two river basins in Iowa and Mississippi SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE agriculture; annual sediment loads; scale effect; sediment yield; soil management practices ID EFFECTS ASSESSMENT PROJECT; ESTIMATING SUSPENDED SEDIMENT; CONSERVATION PRACTICES; METHODOLOGICAL CONSIDERATIONS; BRITISH-COLUMBIA; DELIVERY PROBLEM; SOUTHERN BRAZIL; TRACE-ELEMENT; RATING CURVES; UNITED-STATES AB The specific sediment yield (SSY) from watersheds is the result of the balance between natural, scale-dependent erosion and deposition processes, but can be greatly altered by human activities. In general, the SSY decreases along the course of a river as sediments are trapped in alluvial plains and other sinks. However, this relation between SSY and basin area can actually be an increasing one when there is a predominance of channel erosion relative to hillslope erosion. The US Geological Survey (USGS) conducted a study of suspended sediment in the Iowa River basin (IRB), Iowa, and the Yazoo River basin (YRB), Mississippi, from 2006 through 2008. Within each river basin, the SSY from four largely agricultural watersheds of various sizes (2.3 to 35,000 km(2) [0.9 to 13,513 mi(2)]) was investigated. In the smallest watersheds,YRB sites had greater SSY compared to IRB sites due to higher rain erosivity, more erodible soils, more overland flow, and fluvial geomorphological differences. Watersheds in the YRB showed a steady decrease in SSY with increasing drainage basin area, whereas in the IRB, the maximum SSY occurred at the 30 to 500 km(2) (11.6 to 193 mi(2)) scale. Subsurface tile drainage and limits to channel downcutting restrict the upstream migration of sediment sources in the IRB. Nevertheless, by comparing the SSY-basin size scaling relationships with estimated rates of field erosion under conservation and conventional tillage treatments reported in previous literature, we show evidence that the SSY-basin size relationship in both the IRB and YRB remain impacted by historical erosion rates that occurred prior to conservation efforts. C1 [Merten, G. H.] Univ Minnesota, Dept Civil Engn, Duluth, MN 55812 USA. [Welch, H. L.] US Geol Survey, Lower Mississippi Gulf Water Sci Ctr, Jackson, MS USA. [Tomer, M. D.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. RP Merten, GH (reprint author), Univ Minnesota, Dept Civil Engn, Duluth, MN 55812 USA. NR 73 TC 1 Z9 1 U1 6 U2 10 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD MAY-JUN PY 2016 VL 71 IS 3 BP 267 EP 278 DI 10.2489/jswc.71.3.267 PG 12 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DM3BC UT WOS:000376220800012 ER PT J AU Liu, G Xiao, H Liu, PL Zhang, Q Zhang, JQ AF Liu, Gang Xiao, Hai Liu, Puling Zhang, Qiong Zhang, Jiaqiong TI An improved method for tracing soil erosion using rare earth elements SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Coarse-textured soils; Erosion processes; Particle size distribution; Quantitative information; Sediment sources; Tracers ID 3 GORGES AREA; RILL EROSION; SEDIMENT REDISTRIBUTION; CESIUM-137 MEASUREMENTS; TRACERS; TRANSPORT; RADIONUCLIDES; BERYLLIUM-7; PARTICLES; MOVEMENT AB Purpose The use of rare earth elements (REEs) as tracers provides a high-precision method for quantitative determinations of soil particle movement in soil erosion studies. In this study, a new calculation method was developed and tested to improve the precision of the REE tracing method and to expand the application of this method to areas with coarse-textured soils. Materials and methods This study used purple soil to simulate a catchment with data based on a field survey of a small catchment located in the Three Gorges Area in China. Eight different powdered rare earth oxides, which included La2O3, CeO2, Nd2O3, Sm2O3, Eu2O3, Tb4O7, Ho2O3 and Yb2O3, were applied as tracers to describe soil movement in this scaled catchment during three simulated rainfall events of 1.0, 1.5 and 2.0 mm min(-1) rainfall intensity. Leaching experiments were conducted to investigate the vertical migration of REEs in soil layers. Particle size distributions (PSDs) and REE concentrations for each soil particle size class (1-2, 0.5-1, 0.25-0.5, 0.1-0.25, 0.075-0.1, 0.05-0.075, 0.020.05, 0.005-0.02 and <0.005 mm) were analysed to evaluate the precision of the proposed calculation method. Results and discussion Most REEs remained in the first layer during leaching. The scanning electron microscopy-energy dispersive X-ray (SEM-EDX) mapping images showed that more REEs were adsorbed by small particles (<= 0.1 mm), with large specific surface areas, than by large particles (>0.1 mm). During the three rainfall events, the coarsest size classes (1-2 and 0.5-1 mm) of the sediment samples were less than that of the soil. In contrast, the other classes, including <0.075 mm, showed the strongest adsorption for REEs, and the weight percentage of grains in eroded sediment was more than that in the source soil. The accuracy of the new proposed calculation method increased by 24.37, 20.25 and 3.84% for the first, second and third storm, respectively, compared with the uncorrected mass of soil loss from the scaled catchment. Conclusions The REEs bonded well with purple soil particles and the leaching of REEs from the tagged layer to the lower layers was minimal. The <0.075-mm particle size class had the strongest adsorption capacity for REEs. The soil loss estimates were improved with the new calculation method. C1 [Liu, Gang; Xiao, Hai; Liu, Puling; Zhang, Qiong; Zhang, Jiaqiong] Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Peoples R China. [Liu, Gang] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Liu, G (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Peoples R China.; Liu, G (reprint author), USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. EM gliu@foxmail.com FU National Natural Science Foundation of China [41201270]; Ministry of Science and Technology of China [2015BAC01B03]; West Light Foundation of The Chinese Academy of Sciences [2014-91]; Shaanxi Science and Technology Co-ordination Innovation Engineering Project [2013KTDZ03-03-01]; Scientific Research Foundation of Northwest AF University [2013BSJJ082]; China Scholarship Council [201404910141] FX We thank the editors and two anonymous reviewers for their valuable comments on the manuscript, and we thank Dr. Glenn Wilson for his suggestions and improving the English writing of the manuscript. This research was jointly supported by the National Natural Science Foundation of China (No. 41201270), the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. 2015BAC01B03), the West Light Foundation of The Chinese Academy of Sciences (No. 2014-91), the Shaanxi Science and Technology Co-ordination Innovation Engineering Project (No. 2013KTDZ03-03-01), the Scientific Research Foundation of Northwest A&F University (No. 2013BSJJ082) and China Scholarship Council (No. 201404910141). NR 32 TC 1 Z9 1 U1 13 U2 20 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD MAY PY 2016 VL 16 IS 5 BP 1670 EP 1679 DI 10.1007/s11368-016-1356-y PG 10 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA DM3SA UT WOS:000376265500022 ER PT J AU Drenovsky, RE Thornhill, ML Knestrick, MA Dlugos, DM Svejcar, TJ James, JJ AF Drenovsky, Rebecca E. Thornhill, Megan L. Knestrick, Matthew A. Dlugos, Daniel M. Svejcar, Tony J. James, Jeremy J. TI Seed Production and Seedling Fitness Are Uncoupled from Maternal Plant Productivity in Three Aridland Bunchgrasses SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE fecundity; nitrogen; phosphorus; restoration; water amendment ID SAGEBRUSH STEPPE; GRASSLAND RESTORATION; CHIHUAHUAN DESERT; NITROGEN; GROWTH; SIZE; REPRODUCTION; IRRIGATION; ALLOCATION; GRASSES AB Maintaining self-sustaining populations of desired plants is fundamental to rangeland management, and understanding the relationships among plant growth, seed production, and seedling recruitment is critical to these efforts. Our objective was to evaluate how changes in maternal plant soil resource environment influences maternal plant biomass and seed production and seedling fitness in three widespread perennial bunchgrass species (Elymus elymoides [Raf.] Sezey, bottlebrush squirreltail; Festuca idahoensis Elmer, Idaho fescue; and Pseudoroegneria spicata [Pursh] A. Love, bluebunch wheatgrass). We supplied water and nutrients to adult plants growing in the field and measured their productivity and fecundity. Then, in the laboratory, growth chamber, and field we assessed effects of the maternal water and nutrient additions on offspring performance. Across the three study species, vegetative traits were more plastic than reproductive traits, with resource addition measurably increasing plant growth but not seed production. Germination was high in both the laboratory and field across treatments, although seeds from irrigated maternal plants tended to have higher field germination. Seedling relative growth rate, leaf mass ratio, and relative root elongation rate (RRER) were highly variable, although RRER tended to be higher in seedlings derived from irrigated maternal plants. In the field, seedling survivorship was low across all species, but survivorship doubled in seedlings produced by P. spicata plants that received additional water through the growing season. Overall, our results suggest that biomass production and fecundity responses to nutrients are decoupled in the species and environment tested but maternal effects can have significant, although variable, impacts in some grassland species. As a result, biomass responses to natural and anthropogenic changes in resource availability may not be strong predictors of how altered resource supply may ultimately influence plant community dynamics in aridland systems. (C) 2016 The Society for Range Management. Published by Elsevier Inc. All rights reserved. C1 [Drenovsky, Rebecca E.; Thornhill, Megan L.; Knestrick, Matthew A.; Dlugos, Daniel M.] John Carroll Univ, Dept Biol, 1 John Carroll Blvd, University Hts, OH 44118 USA. [Svejcar, Tony J.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [James, Jeremy J.] Sierra Foothills Res & Extens Ctr, Browns Valley, CA 95918 USA. [Knestrick, Matthew A.; Dlugos, Daniel M.] Univ S Florida, Dept Chem, 4202 E Fowler Ave, Tampa, FL 33620 USA. [Knestrick, Matthew A.; Dlugos, Daniel M.] Michigan State Univ, Dept Plant Soil & Microbial Sci, Plant & Soil Sci Bldg,1066 Bogue St, E Lansing, MI 48824 USA. RP Drenovsky, RE (reprint author), John Carroll Univ, Dept Biol, 1 John Carroll Blvd, University Hts, OH 44118 USA. EM rdrenovsky@jcu.edu FU John Carroll University FX R.E.D. was supported by a John Carroll University Faculty Summer Research Fellowship and Grauel Faculty Fellowship while analyzing the data and writing the manuscript. NR 51 TC 0 Z9 0 U1 17 U2 24 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2016 VL 69 IS 3 BP 161 EP 168 DI 10.1016/j.rama.2016.01.003 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DM3BV UT WOS:000376222700001 ER PT J AU Rinella, MJ Bellows, SE AF Rinella, Matthew J. Bellows, Susan E. TI Evidence-Targeted Grazing Benefits to Invaded Rangelands Can Increase over Extended Time Frames SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE herbivory; invasive weed; leafy spurge; prescribed grazing; simulated grazing; targeted grazing ID STARTHISTLE CENTAUREA-SOLSTITIALIS; LEAFY SPURGE; BIOLOGICAL-CONTROL; EUPHORBIA-ESULA; SHEEP; IDAHO; NORTHERN; CATTLE; GOATS; DEFOLIATION AB Targeted grazing uses livestock to address woody plant encroachment, flammable biomass accumulations, exotic weed invasions, and other management issues. In principle, a feature distinguishing targeted grazing from production-orientated grazing is stocking regimes (i.e., rates, timings, and durations) are chosen to encourage heavy defoliation of unwanted plants at sensitive growth stages. In practice, there are limited data available to guide stocking regime choices. Those data that do exist derive mostly from short-term studies, so the long-term effects of targeted grazing most concerning to managers remain highly uncertain. In a previous study, we imposed clipping treatments to identify defoliation levels and timings effective against the invader leafy spurge (Euphorbia esula L.). Most treatments simulated defoliation by sheep, the animal most commonly used for leafy spurge grazing, though a baseline treatment simulated defoliation by cattle, an animal tending to avoid leafy spurge. The two most effective treatments, which gave similar responses through the end of the previous study, defoliated leafy spurge and other species either before or during leafy spurge flowering. One goal of the current study was to determine if these responses remained similar or diverged over 5 additional treatment years. The other goal was to determine if differences between simulated sheep and cattle grazing treatments increased over time. In the current study, it became increasingly clear that defoliation before flowering was most damaging to leafy spurge, even though defoliation during flowering removed greater leafy spurge biomass. Compared with simulated cattle grazing, simulated sheep grazing before flowering reduced leafy spurge biomass production 74% (52%, 86%) [mean (95% confidence interval)] and increased resident species (mostly grasses) biomass production 40% (14%, 74%) by study's end. Leafy spurge biomass differences between treatments increased gradually over the study period, suggesting long-term research is needed to accurately compare targeted grazing treatments. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Rinella, Matthew J.; Bellows, Susan E.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Rinella, MJ; Bellows, SE (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM Matt.Rinella@ars.usda.gov; Sue.Bellows@ars.usda.gov NR 34 TC 0 Z9 0 U1 11 U2 19 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2016 VL 69 IS 3 BP 169 EP 172 DI 10.1016/j.rama.2016.02.001 PG 4 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DM3BV UT WOS:000376222700002 ER PT J AU Davies, KW Bates, JD Boyd, CS AF Davies, K. W. Bates, J. D. Boyd, C. S. TI Effects of Intermediate-Term Grazing Rest on Sagebrush Communities with Depleted Understories: Evidence of a Threshold SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Artemisia tridentata; defoliation; herbivory; livestock grazing; recovery; restoration ID WYOMING BIG SAGEBRUSH; BIOLOGICAL SOIL CRUSTS; WESTERN NORTH-AMERICA; GREAT-BASIN; PLANT-COMMUNITIES; VEGETATION CHARACTERISTICS; HERBACEOUS UNDERSTORIES; SPECIES ABUNDANCE; VASCULAR PLANTS; BROMUS-TECTORUM AB Millions of hectares of sagebrush (Artemisia L.) plant communities have been degraded by past improper management, resulting in dense sagebrush stands with depleted herbaceous understories. Rest from grazing is often applied to promote recovery. However, the effect of intermediate-term (5-10 years) rest from grazing in sagebrush communities with depleted herbaceous understories and dense sagebrush is relatively unknown. We compared well-managed, moderate grazing (grazed) with intermediate-term (5 and 6 years) rest (ungrazed) at five sites in southeastern Oregon. Sites were Wyoming big sagebrush (Artemisia tridentata Nutt. subsp. wyomingensis Beetle & Young) communities with dense sagebrush and depleted herbaceous understories. Perennial herbaceous cover was greater in ungrazed compared with grazed areas, but this was expected because herbivory removes foliar vegetation tissue (i.e., cover). Density of herbaceous vegetation, diversity, and species richness did not differ between ungrazed and grazed areas. Similarly, bare ground, litter, and biological soil crust cover did not differ between treatments. These results suggest that intermediate-term rest is unlikely to elicit recovery of the understory compared with moderate grazing in these communities. The results of this study also suggest that degraded Wyoming big sagebrush communities likely have crossed a threshold that may be difficult to reverse. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Davies, K. W.; Bates, J. D.; Boyd, C. S.] USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. RP Davies, KW (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu FU USDA-Agricultural Research Service FX Funding for this research project was provided by the USDA-Agricultural Research Service. NR 58 TC 0 Z9 0 U1 5 U2 11 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2016 VL 69 IS 3 BP 173 EP 178 DI 10.1016/j.rama.2016.01.002 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DM3BV UT WOS:000376222700003 ER PT J AU Davies, KW Nafus, AM Boyd, CS Hulet, A Bates, JD AF Davies, K. W. Nafus, A. M. Boyd, C. S. Hulet, A. Bates, J. D. TI Effects of Using Winter Grazing as a Fuel Treatment on Wyoming Big Sagebrush Plant Communities SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Artemisia tridentata; cattle; exclosure; exotic annual grass; fire; fuel treatment ID WESTERN NORTH-AMERICA; BROMUS-TECTORUM L; GREAT-BASIN; SAGE GROUSE; VEGETATION CHARACTERISTICS; BRACHYLAGUS-IDAHOENSIS; ANNUAL GRASS; FIRE; STEPPE; DOMINANCE AB More frequent wildfires and incidences of mega-fires have increased the pressure for fuel treatments in sagebrush (Artemisia) communities. Winter grazing has been one of many fuel treatments proposed for Wyoming big sagebrush (A. tridentata Nutt. subsp. wyomingensis Beetle and A. Young) communities. Though fire risk and severity can be reduced with winter grazing, its impact on vegetation characteristics of Wyoming big sagebrush plant communities is largely unknown. We evaluate the effect of winter grazing at utilization levels between 40% and 60% at five sites in southeastern Oregon. Winter grazing was applied for 5-6 yr before measurements. The winter-grazed and ungrazed treatments generally had similar vegetation characteristics; however, a few characteristics differed. The consumption of prior years' growth resulted in less large perennial bunchgrass, perennial forb, and total herbaceous cover and standing crop and litter biomass. Large perennial bunchgrass and perennial forb density and biomass and exotic annual grass and annual forb cover, density, and biomass did not differ between treatments, suggesting that winter grazing is not negatively impacting resilience and resistance of these communities. Shrub cover was also similar between treatments. These results imply that winter grazing can be applied to reduce fine fuels in Wyoming big sagebrush communities without adversely affecting the native plant community. Winter grazing should, however, be strategically applied because the reduction in perennial grass and perennial forb cover with the consumption of prior years' growth may negatively impact the habitat value for wildlife species that use herbaceous vegetation for concealment. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Davies, K. W.; Boyd, C. S.; Bates, J. D.] USDA ARS, 67826-A Hwy 205, Bums, OR 97720 USA. [Nafus, A. M.] Oregon State Univ, Eastern Oregon Agr Res Serv, Bums, OR 97720 USA. [Hulet, A.] Univ Idaho, Moscow, ID 83844 USA. RP Davies, KW (reprint author), USDA ARS, 67826-A Hwy 205, Bums, OR 97720 USA. EM kirk.davies@oregonstate.edu NR 53 TC 0 Z9 0 U1 5 U2 8 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2016 VL 69 IS 3 BP 179 EP 184 DI 10.1016/j.rama.2015.12.005 PG 6 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DM3BV UT WOS:000376222700004 ER PT J AU Merriam, KE Gosejohan, MC Weisberg, PJ Bovee, KM AF Merriam, K. E. Gosejohan, M. C. Weisberg, P. J. Bovee, K. M. TI Livestock Use Has Mixed Effects on Slender Orcutt Grass in Northeastern California Vernal Pools SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE grazing; Modoc Plateau; Orcuttia tenuis; seasonal wetlands; vernal pool plants ID CLIMATE-CHANGE IMPACTS; EPHEMERAL WETLANDS; CATTLE; ECOSYSTEMS; MANAGEMENT; COMMUNITY; LANDSCAPE; DIVERSITY; DYNAMICS; PLANTS AB Land managers often face the dilemma of balancing livestock use with conservation of sensitive species and ecosystems. For example, most of the remaining vernal pools in California are grazed by livestock. Vernal pools are seasonal wetlands that support many rare and endemic species, such as slender Orcutt grass (Orcuttia tenuis Hitchc.). Although studies in other areas of California have demonstrated that livestock use may benefit some vernal pool specialist species, grazing has been considered a threat to slender Orcutt grass in northeastern California. We evaluated the effects of livestock use on slender Orcutt grass using a replicated, paired design across a range of environmental conditions and grazing management regimes. Frequency, density, cover, reproductive potential, and height of slender Orcutt grass was compared in plots where livestock had been excluded with plots where grazing occurred. We found that livestock do not directly graze slender Orcutt grass, so the effects of livestock use on this species are indirect. These indirect effects are complex, including both positive, neutral, and negative effects. Year had the largest effect on slender Orcutt grass, probably as a result of variation in annual precipitation patterns. Livestock use had no effect in some years; in other years slender Orcutt grass was twice as abundant in unfenced than in fenced plots. Litter cover was also lower in unfenced plots in these years, suggesting that livestock use may benefit slender Orcutt grass in some years by reducing litter accumulation. Conversely, livestock use negatively affected slender Orcutt grass in pastures where livestock hoofprint cover was high, including pastures that were grazed early in the season. By considering patterns of annual variation in environmental factors such as precipitation, site conditions, and season of grazing, land managers can balance the needs of sensitive vernal pool species with maintaining livestock utilization on public lands. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Merriam, K. E.] US Forest Serv, USDA, 159 Lawrence St, Quincy, CA 95971 USA. [Gosejohan, M. C.] Susanville Indian Rancheria, Susanville, CA 96130 USA. [Weisberg, P. J.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Bovee, K. M.] US Forest Serv, USDA, Lassen Natl Forest, Susanville, CA 96130 USA. RP Merriam, KE (reprint author), US Forest Serv, USDA, 159 Lawrence St, Quincy, CA 95971 USA. EM kmerriam@fs.fed.us FU California Department of Fish and Wildlife Lassen-Modoc Special Status Plant Fund; USDA Forest Service; Nevada Agricultural Experiment Station Hatch Grant; University of Nevada Reno; Bureau of Land Management; California Native Plant Society; Northern California Botanists FX Research was funded by the California Department of Fish and Wildlife Lassen-Modoc Special Status Plant Fund, USDA Forest Service, Nevada Agricultural Experiment Station Hatch Grant, University of Nevada Reno, Bureau of Land Management, California Native Plant Society, and Northern California Botanists. NR 43 TC 0 Z9 0 U1 9 U2 15 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2016 VL 69 IS 3 BP 185 EP 194 DI 10.1016/j.rama.2016.01.005 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DM3BV UT WOS:000376222700005 ER PT J AU Hamerlynck, EP Smith, BS Sheley, RL Svejcar, TJ AF Hamerlynck, Erik P. Smith, Brenda S. Sheley, Roger L. Svejcar, Tony J. TI Compensatory Photosynthesis, Water-Use Efficiency, and Biomass Allocation of Defoliated Exotic and Native Bunchgrass Seedlings SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE bluebunch wheatgrass; crested wheatgrass; herbivory; photosynthesis; root-shoot ratios; sagebrush steppe ID COLD-DESERT; WESTERN WHEATGRASS; PLANT-COMMUNITIES; LARGE HERBIVORES; BROMUS-TECTORUM; GROWTH; RESPONSES; DEMOGRAPHY; GRASSES; RESTORATION AB Compensatory increases in net photosynthetic assimilation rates (A(net)) following herbivory are well documented in adult rangeland grasses but have not been quantified in bunchgrass seedlings, which may be more sensitive to tissue loss than established plants. To address this, we twice removed 30% and 70% leaf area of seedlings of crested wheatgrass (Agropyron cristatum [L.] Gaertn., var. Hycrest II) and the native bluebunch wheatgrass (Psuedoroegnaria spicata [Pursh] A. Love, var Anatone) and compared A(net) and aboveground and belowground growth of these to unclipped control plants. Compensatory A(net) occurred only after the second clipping, roughly 1 month after the first, and was similar in magnitude and duration between species and treatments, ca. 26% higher than control plant A(net) for 2 weeks following clipping. Despite similar compensatory A(net) between species, increases in A(net) were more proportional with increased stomatal conductance to water vapor (g(s)) in crested wheatgrass. This resulted in higher intrinsic water use efficiency (WUEi = A(net)/g(s)) integrated across the post-clipping recovery period compared with WUEi of bluebunch seedlings, which declined with clipping. Differences in WUEi were attributable to differences in root-to-shoot ratios and root tissue quality (specific root mass; g dry mass . m(-2) root area), which were lower in crested wheatgrass. We concluded that compensatory photosynthesis is an important component of seedling herbivory tolerance, and that observed differences in post-herbivory WUEi could help improve management strategies by informing seedling selection criteria to help develop methods aimed at minimizing impacts of herbivory during the seedling stage. Published by Elsevier Inc. on behalf of The Society for Range Management. C1 [Hamerlynck, Erik P.; Smith, Brenda S.; Sheley, Roger L.; Svejcar, Tony J.] USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A,Highway 205, Burns, OR 97720 USA. RP Hamerlynck, EP (reprint author), USDA ARS, Eastern Oregon Agr Res Ctr, 67826-A,Highway 205, Burns, OR 97720 USA. EM erik.hamerlynck@oregonstate.edu NR 50 TC 0 Z9 0 U1 8 U2 19 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD MAY PY 2016 VL 69 IS 3 BP 206 EP 214 DI 10.1016/j.rama.2015.12.007 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DM3BV UT WOS:000376222700008 ER PT J AU Flores, D AF Flores, David TI From Prowar Soldier to Antiwar Activist: Change and Continuity in the Narratives of Political Conversion among Iraq War Veterans SO SYMBOLIC INTERACTION LA English DT Article DE conversion; narrative; culture; antiwar veterans; Iraq War ID SOCIAL LOGIC; SOCIOLOGY; MOVEMENT; MEMORY; AWAKENINGS; IDENTITY; VIETNAM; STORIES AB This study examines conversion narratives of Iraq War military veterans who have become antiwar political activists. I examine how antiwar veterans construct and emplot prewar, wartime, and postwar narrative periods to shape and reclaim their moral identities as patriots fighting for a just cause, and how through a communal antiwar story they work to both challenge and reappropriate the rhetorical framework they associate with justifications for the invasion of Iraq. The study draws on in-depth interviews with forty members of Iraq Veterans Against the War (IVAW). In sum, the research describes how veterans engage with dominant narratives, shape new moral identities, and transition from soldiers to political activists. C1 [Flores, David] USDA, US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. RP Flores, D (reprint author), USDA, US Forest Serv, Rocky Mt Res Stn, 333 Broadway SE, Albuquerque, NM 87102 USA. EM davidflores@fs.fed.us NR 54 TC 0 Z9 0 U1 3 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0195-6086 EI 1533-8665 J9 SYMB INTERACT JI Symb. Interact. PD MAY PY 2016 VL 39 IS 2 BP 196 EP 212 DI 10.1002/SYMB.225 PG 17 WC Sociology SC Sociology GA DL8TJ UT WOS:000375914300002 ER PT J AU George, J Robbins, PS Alessandro, RT Stelinski, LL Lapointe, SL AF George, Justin Robbins, Paul S. Alessandro, Rocco T. Stelinski, Lukasz L. Lapointe, Stephen L. TI Formic and Acetic Acids in Degradation Products of Plant Volatiles Elicit Olfactory and Behavioral Responses from an Insect Vector SO CHEMICAL SENSES LA English DT Article DE Asian citrus psyllid; electroantennography; insect feeding behavior; insect-plant interactions ID DIAPHORINA-CITRI KUWAYAMA; HEMIPTERA PSYLLIDAE; ELECTROANTENNOGRAM RESPONSES; TRIOZA-APICALIS; LEAF VOLATILES; HOST PLANTS; ACETALDEHYDE; DISEASE; LEAVES; CUES AB Volatile phytochemicals play a role in orientation by phytophagous insects. We studied antennal and behavioral responses of the Asian citrus psyllid, Diaphorina citri, vector of the citrus greening disease pathogen. Little or no response to citrus leaf volatiles was detected by electroantennography. Glass cartridges prepared with beta-ocimene or citral produced no response initially but became stimulatory after several days. Both compounds degraded completely in air to a number of smaller molecules. Two peaks elicited large antennal responses and were identified as acetic and formic acids. Probing by D. citri of a wax substrate containing odorants was significantly increased by a blend of formic and acetic acids compared with either compound separately or blends containing beta-ocimene and/or citral. Response surface modeling based on a 4-component mixture design and a 2-component mixture-amount design predicted an optimal probing response on wax substrate containing a blend of formic and acetic acids. Our study suggests that formic and acetic acids play a role in host selection by D. citri and perhaps by phytophagous insects in general even when parent compounds from which they are derived are not active. These results have implications for the investigation of arthropod olfaction and may lead to elaboration of attract-and-kill formulations to reduce nontarget effects of chemical control in agriculture. C1 [George, Justin; Robbins, Paul S.; Alessandro, Rocco T.; Lapointe, Stephen L.] USDA ARS, Subtrop Insects & Hort Res Unit, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Stelinski, Lukasz L.] Univ Florida, Citrus Res & Educ Ctr, Entomol & Nematol Dept, 700 Expt Stn Rd, Lake Alfred, FL 33850 USA. RP Lapointe, SL (reprint author), USDA ARS, Subtrop Insects & Hort Res Unit, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM stephen.lapointe@ars.usda.gov NR 60 TC 2 Z9 2 U1 3 U2 15 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0379-864X EI 1464-3553 J9 CHEM SENSES JI Chem. Senses PD MAY PY 2016 VL 41 IS 4 BP 325 EP 338 DI 10.1093/chemse/bjw005 PG 14 WC Behavioral Sciences; Food Science & Technology; Neurosciences; Physiology SC Behavioral Sciences; Food Science & Technology; Neurosciences & Neurology; Physiology GA DK2ZD UT WOS:000374783300006 PM 26857741 ER PT J AU Tisseuil, C Gryspeirt, A Lancelot, R Pioz, M Liebhold, A Gilbert, M AF Tisseuil, Clement Gryspeirt, Aiko Lancelot, Renaud Pioz, Maryline Liebhold, Andrew Gilbert, Marius TI Evaluating methods to quantify spatial variation in the velocity of biological invasions SO ECOGRAPHY LA English DT Article ID SPECIES DISTRIBUTION MODELS; INVADING ORGANISMS; SPREAD; DISPERSAL; DYNAMICS; EPIDEMIC; ACCOUNT; WAVE AB Invading species rarely spread homogeneously through a landscape and invasion patterns typically display irregular frontal boundaries as the invasion progresses through space. Those irregular patterns are generally produced by local environmental factors that may slow or accelerate movement of the frontal boundary. While there is an abundant literature on species distribution modelling methods that quantify local suitability for species establishment, comparatively few studies have examined methods for measuring the local velocity of invasions that can then be statistically analysed in relation to spatially variable environmental factors. Previous studies have used simulations to compare different methods for estimating the overall rate of spread of an invasion. We adopted a similar approach of simulating invasions that resemble two real case-studies, both in terms of their spatial resolution (i.e. considering the size of one cell as one km) and their spatial extent (> 600 000 km(2)). Simulations were sampled to compare how different methods used to measure local spread rate, namely the neighbouring, nearest distance and Delaunay methods, perform for spatio-temporal comparisons. We varied the assessment using three levels of complexity of the spatio-temporal pattern of invasion, three sample sizes (500, 1000 and 2000 points), three different spatial sampling patterns (stratified, random, aggregated), three interpolation methods (generalized linear model, kriging, thin plate spline regression) and two spatio-temporal modelling structures (trend surface analysis and boundary displacement), resulting in a total of 486 different scenarios. The thin plate spline regression interpolation method, in combination with trend surface analysis, was found to provide the most robust local spread rate quantification as it was able to reliably accommodate different sampling conditions and invasion patterns. This best approach was successfully applied to two case-studies, the invasion of France by the horse-chestnut leafminer Cameraria ohridella and by the bluetongue virus, generally in agreement with previously published values of spread rates. Potential avenues for further research are discussed. C1 [Tisseuil, Clement; Gryspeirt, Aiko; Gilbert, Marius] Univ Libre Bruxelles, Biol Control & Spatial Ecol, Av FD Roosevelt 50, BE-1050 Brussels, Belgium. [Gilbert, Marius] Fonds Natl Rech Sci, Rue Egmont 5, BE-1000 Brussels, Belgium. [Lancelot, Renaud] CIRAD, UMR CMAEE, Campus Int Baillarguet,BP, FR-34398 Montpellier, France. [Lancelot, Renaud] INRA, UMR 1309, Campus Int Baillarguet,BP, FR-34398 Montpellier, France. [Pioz, Maryline] INRA, Lab Biol & Protect Abeille, UR Abeilles & Environm 406, Site Agroparc, FR-84914 Avignon, France. [Liebhold, Andrew] USDA, No Res Stn, Forest Serv, Morgantown, WV 26505 USA. RP Gilbert, M (reprint author), Univ Libre Bruxelles, Biol Control & Spatial Ecol, Av FD Roosevelt 50, BE-1050 Brussels, Belgium.; Gilbert, M (reprint author), Fonds Natl Rech Sci, Rue Egmont 5, BE-1000 Brussels, Belgium. EM marius.gilbert@ulb.ac.be FU EU [FP7-261504] FX This study was funded by EU grant FP7-261504 EDENext and is catalogued by the EDENext Steering Committee as EDENext259 (< www.edenext.eu >). The contents of this publication are the sole responsibility of the authors and don't necessarily reflect the views of the European Commission. NR 34 TC 1 Z9 1 U1 7 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-7590 EI 1600-0587 J9 ECOGRAPHY JI Ecography PD MAY PY 2016 VL 39 IS 5 BP 409 EP 418 DI 10.1111/ecog.01393 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DL4JU UT WOS:000375601700001 ER PT J AU Riordan, P Cushman, SA Mallon, D Shi, K Hughes, J AF Riordan, Philip Cushman, Samuel A. Mallon, David Shi, Kun Hughes, Joelene TI Predicting global population connectivity and targeting conservation action for snow leopard across its range SO ECOGRAPHY LA English DT Article ID NORTHERN ROCKY-MOUNTAINS; LANDSCAPE GENETICS; CLIMATE-CHANGE; STEPPING STONES; EXTINCTION RISK; DISPERSAL; HABITAT; SELECTION; VULNERABILITY; PERFORMANCE AB Movements of individuals within and among populations help to maintain genetic variability and population viability. Therefore, understanding landscape connectivity is vital for effective species conservation. The snow leopard is endemic to mountainous areas of central Asia and occurs within 12 countries. We assess potential connectivity across the species' range to highlight corridors for dispersal and genetic flow between populations, prioritizing research and conservation action for this wide-ranging, endangered top-predator. We used resistant kernel modeling to assess snow leopard population connectivity across its global range. We developed an expert-based resistance surface that predicted cost of movement as functions of topographical complexity and land cover. The distribution of individuals was simulated as a uniform density of points throughout the currently accepted global range. We modeled population connectivity from these source points across the resistance surface using three different dispersal scenarios that likely bracket the lifetime movements of individual snow leopard: 100 km, 500 km and 1000 km. The resistant kernel models produced predictive surfaces of dispersal frequency across the snow leopard range for each distance scenario. We evaluated the pattern of connectivity in each of these scenarios and identified potentially important movement corridors and areas where connectivity might be impeded. The models predicted two regional populations, in the north and south of the species range respectively, and revealed a number of potentially important connecting areas. Discrepancies between model outputs and observations highlight unsurveyed areas of connected habitat that urgently require surveying to improve understanding of the global distribution and ecology of snow leopard, and target land management actions to prevent population isolation. The connectivity maps provide a strong basis for directed research and conservation action, and usefully direct the attention of policy makers. C1 [Riordan, Philip; Hughes, Joelene] Univ Oxford, Dept Zool, S Parks Rd, Oxford OX1 3PS, England. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. [Mallon, David] Manchester Metropolitan Univ, Sch Sci & Environm, Div Biol & Conservat Ecol, Manchester M1 5GD, Lancs, England. [Riordan, Philip; Shi, Kun] Beijing Forestry Univ, Coll Nat Conservat, Wildlife Inst, 35 Tsinghua East Rd, Beijing 100083, Peoples R China. RP Riordan, P (reprint author), Univ Oxford, Dept Zool, S Parks Rd, Oxford OX1 3PS, England.; Riordan, P (reprint author), Beijing Forestry Univ, Coll Nat Conservat, Wildlife Inst, 35 Tsinghua East Rd, Beijing 100083, Peoples R China. EM philip.riordan@zoo.ox.ac.uk FU Starr Foundation; Robertson Foundation; Chinese State Forestry Administration; US Dept of Agriculture Forestry Service FX We are grateful for financial support from the Starr Foundation (PR); The Robertson Foundation (PR, JH and KS); The Chinese State Forestry Administration (KS); and the US Dept of Agriculture Forestry Service (SC). NR 65 TC 3 Z9 3 U1 28 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0906-7590 EI 1600-0587 J9 ECOGRAPHY JI Ecography PD MAY PY 2016 VL 39 IS 5 BP 419 EP 426 DI 10.1111/ecog.01691 PG 8 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DL4JU UT WOS:000375601700002 ER PT J AU Herrick, KL Pena, SA Yaglom, HD Layton, BJ Moors, A Loftis, AD Condit, ME Singleton, J Kato, CY Denison, AM Ng, D Mertins, JW Paddock, CD AF Herrick, Kristen L. Pena, Sandra A. Yaglom, Hayley D. Layton, Brent J. Moors, Amanda Loftis, Amanda D. Condit, Marah E. Singleton, Joseph Kato, Cecilia Y. Denison, Amy M. Ng, Dianna Mertins, James W. Paddock, Christopher D. TI Rickettsia parkeri Rickettsiosis, Arizona, USA SO EMERGING INFECTIOUS DISEASES LA English DT Article ID MOUNTAIN-SPOTTED-FEVER; AMBLYOMMA-TRISTE TICKS; UNITED-STATES; ENDEMIC AREA; ARGENTINA; URUGUAY; IXODIDAE; MEXICO; ACARI; IDENTIFICATION AB In the United States, all previously reported cases of Rickettsia parkeri rickettsiosis have been linked to transmission by the Gulf Coast tick (Amblyomma maculatum). Here we describe 1 confirmed and 1 probable case of R. parkeri rickettsiosis acquired in a mountainous region of southern Arizona, well beyond the recognized geographic range of A. maculatum ticks. The likely vector for these 2 infections was identified as the Amblyomma triste tick, a Neotropical species only recently recognized in the United States. Identification of R. parkeri rickettsiosis in southern Arizona demonstrates a need for local ecologic and epidemiologic assessments to better understand geographic distribution and define public health risk. Education and outreach aimed at persons recreating or working in this region of southern Arizona would improve awareness and promote prevention of tickborne rickettsioses. C1 [Herrick, Kristen L.; Yaglom, Hayley D.] Arizona Dept Hlth Serv, Phoenix, AZ 85007 USA. [Pena, Sandra A.] Gila Cty Div Hlth & Emergency Management, Globe, AZ USA. [Layton, Brent J.] Pinal Mt Internal Med, Globe, AZ USA. [Moors, Amanda] Moors Wildlife Management Serv, Globe, AZ USA. [Loftis, Amanda D.] Midwestern Univ, Glendale, AZ USA. [Condit, Marah E.; Singleton, Joseph; Kato, Cecilia Y.; Denison, Amy M.; Ng, Dianna; Paddock, Christopher D.] Ctr Dis Control & Prevent, 1600 Clifton Rd NE,Mailstop G13, Atlanta, GA 30329 USA. [Mertins, James W.] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. RP Paddock, CD (reprint author), Ctr Dis Control & Prevent, 1600 Clifton Rd NE,Mailstop G13, Atlanta, GA 30329 USA. EM cdp9@cdc.gov NR 25 TC 0 Z9 0 U1 3 U2 3 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1080-6040 EI 1080-6059 J9 EMERG INFECT DIS JI Emerg. Infect. Dis PD MAY PY 2016 VL 22 IS 5 BP 780 EP 785 DI 10.3201/eid2205.151824 PG 6 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DK2DZ UT WOS:000374726100002 PM 27089251 ER PT J AU Dill-McFarland, KA Weimer, PJ Pauli, JN Peery, MZ Suen, G AF Dill-McFarland, Kimberly A. Weimer, Paul J. Pauli, Jonathan N. Peery, M. Zachariah Suen, Garret TI Diet specialization selects for an unusual and simplified gut microbiota in two- and three-toed sloths SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ORGANIC-ACID PRODUCTION; GASTROINTESTINAL-TRACT; SP-NOV.; PREVOTELLA-INTERMEDIA; BACTERIAL COMMUNITIES; DIGESTA MOVEMENT; ALOUATTA-PIGRA; GEN. NOV.; RUMEN; CONSERVATION AB Symbiotic microbial communities are critical to the function and survival of animals. This relationship is obligatory for herbivores that engage gut microorganisms for the conversion of dietary plant materials into nutrients such as short-chain organic acids (SCOAs). The constraint on body size imposed by their arboreal lifestyle is thought to make this symbiosis especially important for sloths. Here, we use next-generation sequencing to identify the bacteria present in the fore and distal guts of wild two- and three-toed sloths, and correlate these communities with both diet and SCOAs. We show that, unlike other mammalian herbivores, sloth gut communities are dominated by the bacterial phyla Proteobacteria and Firmicutes. Specifically, three-toed sloths possess a highly conserved, low-diversity foregut community with a highly abundant Neisseria species associated with foregut lactate. In contrast, two-toed sloths have a more variable and diverse foregut microbiota correlated with a variety of SCOAs. These differences support the hypothesis that feeding behaviour selects for specific gut bacterial communities, as three-toed sloths subsist primarily on Cecropia tree leaves while two-toed sloths have a more generalist diet. The less diverse diet and gut microbiota of three-toed sloths may render them more susceptible to habitat loss and other diet-altering conditions. C1 [Dill-McFarland, Kimberly A.; Suen, Garret] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Pauli, Jonathan N.; Peery, M. Zachariah] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Weimer, Paul J.] USDA ARS, Madison, WI 53706 USA. RP Suen, G (reprint author), Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. EM gsuen@wisc.edu FU United States Department of Agriculture (USDA) Agricultural Research Service CRIS project [3655-41000-005-00D]; National Science Foundation [DEB-1257535]; USDA National Institute of Food and Agriculture (NIFA); Milwaukee Public Museum; University of Wisconsin - Madison Graduate School; USDA NIFA [WIS01729] FX The authors thank Jorge Mendoza, Susan Blunck and Dominique Keller for assistance in the field, and all members of the Suen lab for their support, insightful discussions and careful reading of the manuscript. We also thank Holly Hartman and Candace Williams for their advice on statistical analyses. We would also like to thank the students in the Fall 2014 Graduate Topics Course in Species Interactions at the University of Missouri for their critical reading of this manuscript. This work was funded by a United States Department of Agriculture (USDA) Agricultural Research Service CRIS project 3655-41000-005-00D supporting PJW. Funding was provided to MZP and JNP by the National Science Foundation (DEB-1257535), the USDA National Institute of Food and Agriculture (NIFA), the Milwaukee Public Museum, and the University of Wisconsin - Madison Graduate School. Funding was also provided to GS by the USDA NIFA (WIS01729). The authors declare no conflict of interest. NR 75 TC 3 Z9 3 U1 65 U2 74 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD MAY PY 2016 VL 18 IS 5 BP 1391 EP 1402 DI 10.1111/1462-2920.13022 PG 12 WC Microbiology SC Microbiology GA DL2QX UT WOS:000375481200010 PM 26271635 ER PT J AU Su, S Miska, KB Fetterer, RH Jenkins, MC Wong, EA AF Su, S. Miska, K. B. Fetterer, R. H. Jenkins, M. C. Wong, E. A. TI Expression of digestive enzymes and nutrient transporters in Eimeria-challenged broilers (vol 150, pg 13, 2015) SO EXPERIMENTAL PARASITOLOGY LA English DT Correction C1 [Su, S.; Wong, E. A.] Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. [Miska, K. B.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD USA. [Fetterer, R. H.; Jenkins, M. C.] USDA ARS, Anim Parasit Dis Lab, Beltsville, MD USA. RP Wong, EA (reprint author), Virginia Tech, Dept Anim & Poultry Sci, Blacksburg, VA 24061 USA. EM ewong@vt.edu NR 1 TC 0 Z9 0 U1 2 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0014-4894 EI 1090-2449 J9 EXP PARASITOL JI Exp. Parasitol. PD MAY PY 2016 VL 164 BP 97 EP 98 DI 10.1016/j.exppara.2016.02.006 PG 2 WC Parasitology SC Parasitology GA DJ8AY UT WOS:000374435700015 PM 27059197 ER PT J AU Koppelman, SJ Jayasena, S Luykx, D Schepens, E Apostolovic, D de Jong, GAH Isleib, TG Nordlee, J Baumert, J Taylor, SL Cheng, H Maleki, S AF Koppelman, Stef J. Jayasena, Shyamali Luykx, Dion Schepens, Erik Apostolovic, Danijela de Jong, Govardus A. H. Isleib, Thomas G. Nordlee, Julie Baumert, Joe Taylor, Steve L. Cheng, Hsiaopo Maleki, Soheila TI Allergenicity attributes of different peanut market types SO FOOD AND CHEMICAL TOXICOLOGY LA English DT Article DE Peanut; Arachis hypogaea; Allergen; Immunoassay; IgE ID ARA H 2; ARACHIS-HYPOGAEA L.; SUBLINGUAL IMMUNOTHERAPY; MULTICENTER TRIAL; DOUBLE-BLIND; IGE-BINDING; ALLERGY; PROTEINS; REDUCTION; EXTRACTS AB Four different market classes of peanut (Runner, Virginia Spanish, and Valencia) are commonly consumed in Western countries, but for some consumers peanuts are a main cause of food-induced anaphylaxis. Limited information is available on the comparative allergenicity of these distinct market classes. The aim of this study was to compare allergenicity attributes of different peanut cultivars. The protein content and protein profiles were highly comparable for all tested cultivars. All cultivar samples contained the major allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6, as assessed by SOS-PAGE and RP-HPLC, although some minor differences in major allergen content were found between samples. All samples were reactive in commercial ELISAs for detection and quantification of peanut protein. IgE-binding potency differed between samples with a maximum factor of 2, indicating a highly comparable allergenicity. Based on our observations, we conclude that peanuts from the main market types consumed in Western countries are highly comparable in their allergenicity attributes, indicating that safety considerations with regard to peanut allergy are not dependent on the peanut cultivar in question. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Koppelman, Stef J.; Jayasena, Shyamali; Nordlee, Julie; Baumert, Joe; Taylor, Steve L.] Univ Nebraska, Dept Food Sci & Technol, Food Allergy Res & Resource Program, 279 Food Innovat Ctr, Lincoln, NE 68588 USA. [Luykx, Dion; Schepens, Erik; Apostolovic, Danijela] HAL Allergy, JH Oortweg 15-17, NL-2333 CH Leiden, Netherlands. [de Jong, Govardus A. H.] TNO, Utrechtseweg 48, NL-3704 HE Zeist, Netherlands. [Isleib, Thomas G.] N Carolina State Univ, 210 Method Rd,Greenhouse Unit 3, Raleigh, NC 27695 USA. [Cheng, Hsiaopo; Maleki, Soheila] ARS, USDA, Food Allergy Res, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Apostolovic, Danijela] Karolinska Inst, Clin Immunol & Allergy Unit, Dept Med Solna, Stockholm, Sweden. RP Koppelman, SJ (reprint author), Univ Nebraska, Food Allergy Res & Resource Program, Lincoln, NE 68588 USA. EM stefkoppelman@zonnet.nl RI Apostolovic, Danijela/Q-9067-2016 OI Apostolovic, Danijela/0000-0001-8388-6916 NR 46 TC 3 Z9 3 U1 11 U2 21 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0278-6915 EI 1873-6351 J9 FOOD CHEM TOXICOL JI Food Chem. Toxicol. PD MAY PY 2016 VL 91 BP 82 EP 90 DI 10.1016/j.fct.2016.02.016 PG 9 WC Food Science & Technology; Toxicology SC Food Science & Technology; Toxicology GA DL4TD UT WOS:000375629200009 PM 26921497 ER PT J AU Cortes-Avizanda, A Blanco, G DeVault, TL Markandya, A Virani, MZ Brandt, J Donazar, JA AF Cortes-Avizanda, Ainara Blanco, Guillermo DeVault, Travis L. Markandya, Anil Virani, Munir Z. Brandt, Joseph Donazar, Jose A. TI Supplementary feeding and endangered avian scavengers: benefits, caveats, and controversies SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Review ID VULTURE GYPAETUS-BARBATUS; SPANISH IMPERIAL EAGLE; FACULTATIVE SCAVENGERS; ECOSYSTEM SERVICES; SANITARY POLICIES; BEARDED VULTURES; BREEDING SUCCESS; ASIAN VULTURES; GYPS-FULVUS; CONSERVATION AB Large avian scavengers are among the most vulnerable vertebrates, and many of their populations have declined severely in recent decades. To help mitigate this marked reduction in abundance, supplementary feeding stations (SFS; colloquially termed "vulture restaurants") have been created worldwide, often without consideration of the scientific evidence supporting the suitability of the practice. SFS have been effective and important tools for conservation and reintroduction of avian scavengers. However, negative consequences can result from large aggregations of individual birds, disrupting intraguild processes and promoting density dependent decreases in productivity. At the community level, SFS favor the congregation of predators (ie facultative scavengers), increasing predation risk on small-and medium-sized vertebrates in the vicinity of the SFS. These feeding stations might also affect processes of natural selection and even render populations maladapted to their natural environments. We also examine future scenarios for avian scavengers in relation to ecosystem services, to changes in agro-grazing economies and in land uses, and ultimately to rewilding landscapes where SFS play a controversial role. C1 [Cortes-Avizanda, Ainara] Infraestruturas Portugal Biodivers Chair CIBIO In, Campus Agr Vairao, Vairao, Portugal. [Cortes-Avizanda, Ainara; Donazar, Jose A.] CSIC, Dept Conservat Biol, Estn Biol Donana, E-41080 Seville, Spain. [Blanco, Guillermo] CSIC, Dept Evolutionary Ecol, Museo Nacl Ciencias Nat, Madrid, Spain. [DeVault, Travis L.] USDA, Natl Wildlife Res Ctr, Sandusky, OH USA. [Markandya, Anil] Basque Ctr Climate Change, Bilbao, Spain. [Virani, Munir Z.] Peregrine Fund, Boise, ID USA. [Virani, Munir Z.] Natl Museums Kenya, Ornithol Sect, Dept Zool, Nairobi, Kenya. [Brandt, Joseph] US Fish & Wildlife Serv, Calif Condor Recovery Program, Hopper Mt Natl Wildlife Refuge, Ventura, CA USA. RP Cortes-Avizanda, A (reprint author), Infraestruturas Portugal Biodivers Chair CIBIO In, Campus Agr Vairao, Vairao, Portugal.; Cortes-Avizanda, A (reprint author), CSIC, Dept Conservat Biol, Estn Biol Donana, E-41080 Seville, Spain. EM cortesavizanda@gmail.com RI CSIC, EBD Donana/C-4157-2011; Evolutionary Ecology, Ecologia Evolutiva/M-3553-2014; OI CSIC, EBD Donana/0000-0003-4318-6602; CORTES-AVIZANDA, AINARA/0000-0002-9674-6434 FU [CGL2012-40013-C01]; [CGL2010-15726]; [RNM-1925]; [FCT-SFRH/BPD/91609/2012] FX This work was funded by the projects CGL2012-40013-C01, CGL2010-15726, and RNM-1925. ACA was supported by a postdoctoral grant FCT-SFRH/BPD/91609/2012. I Afan (LAST-EBD) and M de la Riva assisted with map and Figure 2 creation, respectively. NR 58 TC 4 Z9 4 U1 10 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD MAY PY 2016 VL 14 IS 4 BP 191 EP 199 DI 10.1002/fee.1257 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DL5KP UT WOS:000375676200016 ER PT J AU Montalva, C Collier, K Rocha, LFN Inglis, PW Lopes, RB Luz, C Humber, RA AF Montalva, Cristian Collier, Karin Nunes Rocha, Luiz Fernando Inglis, Peter Ward Lopes, Rogerio Biaggioni Luz, Christian Humber, Richard A. TI A natural fungal infection of a sylvatic cockroach with Metarhizium blattodeae sp nov., a member of the M. flavouiride species complex SO FUNGAL BIOLOGY LA English DT Article DE Blattodea; Brazil; Clavicipitaceae; Entomopathogenic fungus; Goias ID GERMAN-COCKROACH; DICTYOPTERA BLATTELLIDAE; HORIZONTAL TRANSMISSION; PERIPLANETA-AMERICANA; MULTILOCUS PHYLOGENY; ANISOPLIAE; DEUTEROMYCOTINA; SYNERGISM; PATHOGEN AB A wild, forest-dwelling cockroach from the subfamily Ectobiidae (order Blattodea) in a nature reserve in Cavalcante, in the state of Goias, Brazil, was found to be infected by a new, genetically distinct species in the Metarhizium flavoviride species complex that we describe here as Metarhizium blattodeae. The status of this fungus as a new species is supported by both multigenic sequence comparisons and protein profiles generated by MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry. This is one of the first reports of a naturally occurring fungal pathogen affecting any sylvatic (forest-dwelling) cockroach from any part of the world. M. blattodeae caused up to 96 % mortality of Periplaneta americana nymphs (a serious peridomestic cockroach species) after 10 d. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Montalva, Cristian; Nunes Rocha, Luiz Fernando; Luz, Christian] Univ Fed Goias, IPTSP, BR-74605050 Goiania, Go, Brazil. [Collier, Karin] Ctr Univ Gurupi UnirG, BR-77425500 Gurupi, Tocantins, Brazil. [Nunes Rocha, Luiz Fernando] Inst Fed Educ Ciencia & Tecnol Goias IFG, BR-74968755 Aparecida De Goiania, Go, Brazil. [Inglis, Peter Ward; Lopes, Rogerio Biaggioni] Embrapa Recursos Genet & Biotecnol, BR-70770917 Brasilia, DF, Brazil. [Humber, Richard A.] USDA ARS, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Humber, RA (reprint author), Robert W Holley Ctr Agr & Hlth, Emerging Pests & Pathogens Res, 538 Tower Rd, Ithaca, NY 14853 USA. EM montalva.cristian@gmail.com; karincollier@gmail.com; luizfnr@hotmail.com; peterwinglis@gmail.com; rogerio.lopes@embrapa.br; wchrisluz@hotmail.com; richard.humber@ars.usda.-gov RI Lopes, Rogerio/L-3534-2013 OI Lopes, Rogerio/0000-0003-4756-878X FU CAPES-CSF (Coordination of the Improvement of Higher Education-Science without Borders) [PVE 71/2013, 149988]; IFG-PROAPP [006/2014] FX The authors particularly thank Fabio Padula (Bacupari Reserve, Cavalcante, GO), who provided material assistance in the collection of the Brazilian isolate discussed here. Amanda Cardoso (IPTSP) and Daniela Aguiar Souza (Embrapa, Brasilia) provided technical assistance. This study was supported by CAPES-CSF (Coordination of the Improvement of Higher Education-Science without Borders) project PVE 71/2013, 149988 and IFG-PROAPP 006/2014. NR 39 TC 2 Z9 2 U1 7 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD MAY PY 2016 VL 120 IS 5 BP 655 EP 665 DI 10.1016/j.funbio.2016.03.004 PG 11 WC Mycology SC Mycology GA DL7HV UT WOS:000375812700001 PM 27109363 ER PT J AU Xia, CJ Wan, AM Wang, MN Jiwan, DA See, DR Chen, XM AF Xia, Chongjing Wan, Anmin Wang, Meinan Jiwan, Derick A. See, Deven R. Chen, Xianming TI Secreted protein gene derived-single nucleotide polymorphisms (SP-SNPs) reveal population diversity and differentiation of Puccinia striiformis f. sp tritici in the United States SO FUNGAL BIOLOGY LA English DT Article DE Heterozygosity; Population differentiation; Wheat stripe rust ID WHEAT STRIPE RUST; US PACIFIC-NORTHWEST; ALTERNATE HOST; F.SP TRITICI; MICROSATELLITE LOCI; MOLECULAR ECOLOGY; VIRULENCE VARIATION; YELLOW RUST; PATHOGEN; SEQUENCE AB Single nucleotide polymorphism (SNP) is a powerful molecular marker technique that has been widely used in population genetics and molecular mapping studies for various organisms. However, the technique has not been used for studying Puccinia striiformis f. sp. tritici (Pst), the wheat stripe rust pathogen. In this study, we developed over a hundred secreted protein gene-derived SNP (SP-SNP) markers and used 92 markers to study the population structure of Pst. From 352 isolates collected in the United States, we identified 242 multi-locus genotypes. The SP-SNP genotypes had a moderate, but significant correlation with the virulence phenotype data. Clustering of the multi-locus genotypes was consistent by various analyses, revealing distinct genetic groups. Analysis of molecular variance detected significant differences between the eastern and western US Pst populations. High heterozygosity was found in the US population with significant differences identified among epidemiological regions. Analysis of population differentiation revealed that populations between the eastern and western US were highly differentiated while moderate differentiation was found in populations within the western or eastern US. Isolates from the western US were more diverse than isolates from the eastern US. The information is useful for guiding the disease management in different epidemiological regions. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Xia, Chongjing; Wan, Anmin; Wang, Meinan; Jiwan, Derick A.; See, Deven R.; Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [See, Deven R.; Chen, Xianming] ARS, USDA, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. RP Chen, XM (reprint author), ARS, USDA, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. EM chongjing.xia@wsu.edu; awan@wsu.edu; meinan_wang@wsu.edu; djiwan@wsu.edu; deven_see@wsu.edu; xianming@wsu.edu FU U.S. Department of Agriculture, Agricultural Research Service [5348-22000-015-00D]; Washington State University [13C-3061-5665]; PPNS [0690]; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences; Agricultural Research Center; HATCH Project [WNP00663]; Washington State University, Pullman, WA, USA [99164-6430]; Chinese Scholarship Council (CSC) FX This research was supported by the U.S. Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-015-00D) and Washington State University (13C-3061-5665) PPNS No. 0690, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, HATCH Project Number WNP00663, Washington State University, Pullman, WA 99164-6430, USA. The Chinese Scholarship Council (CSC) scholarship given to Chongjing Xia is highly appreciated. We are grateful to Drs Scot Hubert and Tobin Peever for critical review of the manuscript. NR 77 TC 1 Z9 1 U1 6 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD MAY PY 2016 VL 120 IS 5 BP 729 EP 744 DI 10.1016/j.funbio.2016.02.007 PG 16 WC Mycology SC Mycology GA DL7HV UT WOS:000375812700007 PM 27109369 ER PT J AU Parsa, S Garcia-Lemos, AM Castillo, K Ortiz, V Lopez-Lavalle, LAB Braun, J Vega, FE AF Parsa, Soroush Garcia-Lemos, Adriana M. Castillo, Katherine Ortiz, Viviana Becerra Lopez-Lavalle, Luis Augusto Braun, Jerome Vega, Fernando E. TI Fungal endophytes in germinated seeds of the common bean, Phaseolus vulgaris SO FUNGAL BIOLOGY LA English DT Article DE Aureobasidium pullulans; Biological control; Endophytic; Fungal biology; Seed-borne fungi ID AUREOBASIDIUM-PULLULANS; BIOLOGICAL-CONTROL; BOTRYTIS-CINEREA; MOLECULAR CHARACTERIZATION; BACTERIAL ENDOPHYTES; FUSARIUM-OXYSPORUM; POSTHARVEST ROTS; DIVERSITY; SYMBIOSIS; COLONIZATION AB We conducted a survey of fungal endophytes in 582 germinated seeds belonging to 11 Colombian cultivars of the common bean (Phaseolus vulgaris). The survey yielded 394 endophytic isolates belonging to 42 taxa, as identified by sequence analysis of the ribosomal DNA internal transcribed spacer (ITS) region. Aureobasidium pullulans was the dominant endophyte, isolated from 46.7 % of the samples. Also common were Fusarium oxysporum, Xylaria sp., and Cladosporium cladosporioides, but found in only 13.4 %, 11.7 %, and 7.6 % of seedlings, respectively. Endophytic colonization differed significantly among common bean cultivars and seedling parts, with the highest colonization occurring in the first true leaves of the seedlings. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Parsa, Soroush] Univ Calif Davis Chile, Life Sci Innovat Ctr, Andres Bello 2299 1102, Santiago, Chile. [Parsa, Soroush; Garcia-Lemos, Adriana M.; Castillo, Katherine; Ortiz, Viviana; Becerra Lopez-Lavalle, Luis Augusto] Ctr Int Agr Trop, Apartado Aereo 6713, Cali, Colombia. [Braun, Jerome] 3034 Boulder Pl, Davis, CA 95618 USA. [Vega, Fernando E.] ARS, Sustainable Perennial Crops Lab, USDA, BARC W, Bldg 001, Beltsville, MD 20705 USA. RP Vega, FE (reprint author), ARS, Sustainable Perennial Crops Lab, USDA, BARC W, Bldg 001, Beltsville, MD 20705 USA. EM Fernando.Vega@ars.usda.gov OI Castillo-Avila, Diana Katherine/0000-0002-0950-0074; Becerra Lopez-Lavalle, Luis Augusto/0000-0003-3520-2270; Garcia, Adriana/0000-0003-1281-3335; Vega, Fernando E./0000-0001-8103-5640 FU Bill and Melinda Gates Foundation Grand Challenges Exploration grant [OPP1069291]; Colombian Administrative Department of Science, Technology and Innovation (Colciencias) grant [2236-521-28463] FX This project was supported by a Bill and Melinda Gates Foundation Grand Challenges Exploration grant (#OPP1069291, Endophytic biological control for cassava and beans) to S. Parsa and F.E. Vega, and by a Colombian Administrative Department of Science, Technology and Innovation (Colciencias) grant (#2236-521-28463) to S. Parsa. NR 63 TC 1 Z9 1 U1 9 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD MAY PY 2016 VL 120 IS 5 BP 783 EP 790 DI 10.1016/j.funbio.2016.01.017 PG 8 WC Mycology SC Mycology GA DL7HV UT WOS:000375812700012 PM 27109374 ER PT J AU Zhao, Y Davis, RE AF Zhao, Yan Davis, Robert E. TI Criteria for phytoplasma 16Sr group/subgroup delineation and the need of a platform for proper registration of new groups and subgroups SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID WITCHES-BROOM DISEASE; RIBOSOMAL-RNA; X-DISEASE; CLASSIFICATION; IDENTIFICATION; DIVERSITY; GENES AB As phytoplasmas are discovered at an ever-increasing pace in emerging and re-emerging plant diseases worldwide, the scheme for classification of phytoplasmas into 16S rRNA gene RFLP (16Sr) groups and subgroups is experiencing an ongoing rapid expansion. Improper delineation or designation of new groups and subgroups can open potential conflicts in classifying newly identified phytoplasma strains. To maintain the integrity of the classification scheme, criteria for the delineation of new groups and subgroups must be followed, and proper registration should be required to track established groups and subgroups. C1 [Zhao, Yan; Davis, Robert E.] USDA ARS, Mol Plant Pathol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Zhao, Y (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM yan.zhao@ars.usda.gov NR 21 TC 2 Z9 2 U1 6 U2 10 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD MAY PY 2016 VL 66 BP 2121 EP 2123 DI 10.1099/ijsem.0.000999 PN 5 PG 3 WC Microbiology SC Microbiology GA DL7RF UT WOS:000375837100035 PM 26928977 ER PT J AU Lima, I Eggleston, G Sarir, E Donado, CA Thompson, J St Cyr, E AF Lima, Isabel Eggleston, Gillian Sarir, Emmanuel Donado, Carlos Andres Thompson, Jack St Cyr, Eldwin TI Mechanism of removal of undesirable residual amylase, insoluble starch, and select colorants from refinery streams by powdered activated carbons SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE activated carbon; white sugar; refined sugar; amylase activity; insoluble starch; colorant; Phadebas (R) ID ALPHA-AMYLASE AB There is a need in the world-wide sugar industry to find a practical and economical solution to remove or inactivate residual alpha-amylases that are high temperature stable from factory or refinery streams. A survey of refineries that used amylase and had activated carbon systems for decolorization, revealed they did not have any customer complaints for residual amylase. Five commercial, high performance powdered activated carbons (PAC) were investigated for their ability to remove residual amylase as well as other impurities. Removal of the residual amylase protein by PAC was dependent on its surface area as well as mixing (retention) time. A select, high performance PAC (RFM-JH (TM)) with high surface area (1764 m(2)/g) performed the best and, because this PAC also had the largest particle size characteristics (mean particle size similar to 274 mu m), it was also the easiest to filter after use. Activated carbon also had the additional benefit of removing color, and RFM-JH (TM) preferentially removed cane derived flavonoid and phenolic colorants at pH 9 over process formed color at pH 4, which is opposite of color removal by the refinery phosphatation-clarification process and thus complementary. The dose of RFM-JH (TM) was more critical than retention time with respect to color removal. RFM-JH (TM) also consistently removed undesirable insoluble starch from raw sugar syrups and refinery liquors. A tentative mechanism for the removal of impurities is provided. Further studies are now warranted to investigate the use of high performance PAC as a tool to remove residual amylase, select colorants, and insoluble starch at the large-scale. C1 [Lima, Isabel; Eggleston, Gillian; St Cyr, Eldwin] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Sarir, Emmanuel] CarboUA Int, Los Angeles, CA USA. [Donado, Carlos Andres] CarboUA Int, Sao Paulo, Brazil. [Thompson, Jack] LSR Refinery LLC, 1189 South Fifth Ave, Gramercy, LA 70052 USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov NR 30 TC 0 Z9 0 U1 1 U2 2 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD MAY PY 2016 VL 118 IS 1409 BP 352 EP 362 PG 9 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA DL3DA UT WOS:000375513200036 ER PT J AU Ahlberg, CM Kuehn, LA Thallman, RM Kachman, SD Snelling, WM Spangler, ML AF Ahlberg, C. M. Kuehn, L. A. Thallman, R. M. Kachman, S. D. Snelling, W. M. Spangler, M. L. TI Breed effects and genetic parameter estimates for calving difficulty and birth weight in a multibreed population SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beef cattle; breed effects; calving difficulty ID EXPECTED PROGENY DIFFERENCES; LONGISSIMUS PALATABILITY TRAITS; BIOLOGICAL TYPES; BEEF-CATTLE; POSTWEANING GAIN; GESTATION LENGTH; WEANING TRAITS; CARCASS TRAITS; CALF GROWTH; BRAHMAN AB Birth weight (BWT) and calving difficulty (CD) were recorded on 4,579 first-parity females from the Germplasm Evaluation Program at the U.S. Meat Animal Research Center (USMARC). Both traits were analyzed using a bivariate animal model with direct and maternal effects. Calving difficulty was transformed from the USMARC scores to corresponding Z-scores from the standard normal distribution based on the incidence rate of the USMARC scores. Breed fraction covariates were included to estimate breed differences. Heritability estimates (SE) for BWT direct, CD direct, BWT maternal, and CD maternal were 0.34 (0.10), 0.29 (0.10), 0.15 (0.08), and 0.13 (0.08), respectively. Calving difficulty direct breed effects deviated from Angus ranged from -0.13 to 0.77 and maternal breed effects deviated from Angus ranged from -0.27 to 0.36. Hereford-, Angus-, Gelbvieh-, and Brangus-sired calves would be the least likely to require assistance at birth, whereas Chiangus-, Charolais-, and Limousin-sired calves would be the most likely to require assistance at birth. Maternal breed effects for CD were least for Simmental and Charolais and greatest for Red Angus and Chiangus. Results showed that the diverse biological types of cattle have different effects on both BWT and CD. Furthermore, results provide a mechanism whereby beef cattle producers can compare EBV for CD direct and maternal arising from disjoined and breed-specific genetic evaluations. C1 [Ahlberg, C. M.; Spangler, M. L.] Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. [Kuehn, L. A.; Thallman, R. M.; Snelling, W. M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Kachman, S. D.] Univ Nebraska, Dept Stat, Lincoln, NE USA. RP Spangler, ML (reprint author), Univ Nebraska, Dept Anim Sci, Lincoln, NE 68583 USA. EM mspangler2@unl.edu OI Spangler, Matthew/0000-0001-5184-501X; Kachman, Stephen/0000-0003-0506-513X NR 29 TC 1 Z9 1 U1 2 U2 2 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2016 VL 94 IS 5 BP 1857 EP 1864 DI 10.2527/jas2015-0161 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XL UT WOS:000375783300009 PM 27285683 ER PT J AU Hales, KE Shackelford, SD Wells, JE King, DA Pyatt, NA Freetly, HC Wheeler, TL AF Hales, K. E. Shackelford, S. D. Wells, J. E. King, D. A. Pyatt, N. A. Freetly, H. C. Wheeler, T. L. TI Effects of dietary protein concentration and ractopamine hydrochloride on performance and carcass characteristics of finishing beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE beta-agonist; carcass quality; dietary protein; finishing cattle ID GROWTH-PERFORMANCE; HYDROGEN-CHLORIDE; FEEDLOT HEIFERS; MEAT QUALITY; OPTAFLEXX; CATTLE; TENDERNESS AB Ractopamine hydrochloride (RAC) is used in the feedlot industry to increase daily gain, improve feed efficiency, and increase HCW. However, little work has been done to determine whether additional protein is needed in the diet to maximize the benefit of RAC in beef cattle. Objectives of our experiment were to determine if feeding additional CP in conjunction with RAC would improve animal performance and carcass characteristics. Therefore, an experiment was conducted using finishing diets containing 13.5 or 17.5% CP with 0 or 300 mg of RAC for 30 to 33 d at the end of the finishing period. Beef steers (n = 438; 387.8 +/- 1.9 kg initial BW) were randomly assigned to 1 of 4 treatments in a 2 x 2 factorial arrangement (16 pens total, 4 pens/treatment). No interactions between feeding RAC and CP level were detected (P > 0.19) for animal performance or carcass traits. Final BW did not differ among treatment (P = 0.37); however, final BW had a tendency to be 2% greater (P = 0.07) when the 13.5% CP diet was fed. Dry matter intake was not different between steers fed 0 or 300 mg/d-1 of RAC (P = 0.20), yet DMI was 12% greater for cattle fed the 13.5% CP compared with steers fed the 17.5% CP diet (P < 0.01). Daily gain did not differ for cattle fed different levels of RAC or CP (P > 0.16). The G: F was 3.6% greater for cattle fed 300 vs. 0 mg/d(-1) of RAC (P = 0.04). The G:F was 8.7% greater for cattle fed the 17.5% diet vs. the 13.5% CP diet (P < 0.01), which can be attributed to the decreased DMI for cattle fed the 17.5% CP diet. Hot carcass weight was not different for steers fed 0 or 300 mg/d(-1) of RAC (P = 0.36) or for steers fed the 13.5% diet vs. 17.5% CP diet (P = 0.93). Dressing percentage was 1.5% greater for cattle fed 300 vs. 0 mg/d(-1) of RAC (P = 0.05) but was not different between cattle fed the different CP levels in the diet (P = 0.16). Longissimus area, adjusted 12th-rib fat, and marbling score did not differ across RAC or CP treatments (P > 0.26). Additionally, no differences in USDA yield grade or percentage of cattle grading USDA Choice were detected for RAC or CP treatments (P > 0.26), which also supports the idea that quality grade of cattle fed RAC at the same level of fatness is not impacted. Our data indicate excess protein did not enhance the response to RAC, and furthermore, the improved performance from RAC reported by others was not observed other than a small increase in G:F. C1 [Hales, K. E.; Shackelford, S. D.; Wells, J. E.; King, D. A.; Freetly, H. C.; Wheeler, T. L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Pyatt, N. A.] Elanco Anim Hlth, Greenfield, IN 46140 USA. RP Hales, KE (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Kristin.Hales@ars.usda.gov NR 20 TC 0 Z9 0 U1 6 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2016 VL 94 IS 5 BP 2097 EP 2102 DI 10.2527/jas2015-0225 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XL UT WOS:000375783300032 PM 27285706 ER PT J AU Sapkota, A Marchant-Forde, JN Richert, BT Lay, DC AF Sapkota, A. Marchant-Forde, J. N. Richert, B. T. Lay, D. C., Jr. TI Including dietary fiber and resistant starch to increase satiety and reduce aggression in gestating sows SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE aggression; dietary fiber; resistant starch; satiety; sow; welfare ID GROUP-HOUSED SOWS; FLOOR SPACE ALLOWANCE; GROUP-SIZE; REPRODUCTIVE-PERFORMANCE; PREGNANT SOWS; AD-LIBITUM; PEN SIZE; BEHAVIOR; PIGS; WELFARE AB Aggression during mixing of pregnant sows impacts sow welfare and productivity. The aim of this study was to increase satiety and reduce aggression by including dietary fiber and fermentable carbohydrates. Sows were housed in individual stalls 7 to 14 d after breeding (moving day was considered d 0 of treatment) and were fed (at 0700 h) with a CONTROL (corn-soybean meal based with no additional fiber sources), RSTARCH (10.8% resistant starch), BEETPULP (27.2% sugar beet pulp), SOYHULLS (19.1% soybean hulls), or INCSOY (14.05% soybean hulls) for 21 d (5 sows/diet x 5 diets x 8 replications = 200 sows). The CONTROL diet was targeted to contain 185 g(d.sow) NDF and the other diets were targeted to contain 350 g(d sow) NDF. The INCSOY diet was fed at 2.2 kg/(d.sow) and the other diets were fed at 2 kg(d sow). On d 22, sows were mixed in groups of 5 (at 1200 h). Behaviors in stalls (on d 1, 7, 14, and 21) and after mixing (d 22 and 23), heart rate (on d 1, 7, 14, and 21), blood metabolites (on d 2, 8, 15, 22, and 25), and the effects of diets on production were collected and analyzed. Sows stood more (P < 0.01) and rested less (P < 0.001) over time irrespective of the diet. Sows on BEETPULP stood more (P < 0.01) and sows on SOYHULLS rested more (P < 0.01). Sham chewing increased over days irrespective of the diet. Chewing behavior (bar and feeder) increased with days on diet (P < 0.001) and was lowest in sows on the SOYHULLS diet (P = 0.045). When mixed, biting frequency in the first hour was highest for sows on the CONTROL diet (236.5 +/- 62.6) and lowest for sows on the RSTARCH diet (90.5 +/- 30.5). Skin lesions increased (P < 0.001) 24 h after mixing sows irrespective of diet. Blood urea nitrogen (BUN) concentration was lowest in sows fed BEETPULP and SOYHULLS (P < 0.001). Serum glucose concentration was highest in sows fed RSTARCH and BEETPULP (P = 0.04), but there was no day effect (P = 0.62) or diet x day interaction (P = 0.60). The NEFA was greatest in sows fed RSTARCH, BEETPULP, and SOYHULLS (P < 0.001). Lactate (P < 0.001) and BUN concentrations were greatest on d 2 but dropped and remained constant after d 8. Average heart rate was lowest for sows on SOYHULLS and INCSOY compared with sows on the other diets (P = 0.03). Number of piglets born and average weaning weight were not affected by diets (P > 0.05). Average birth weight was lowest in the INCSOY diet (P = 0.02). This study demonstrates that RSTARCH and SOYHULLS can improve the welfare of sows by reducing aggression and increasing satiety in limit-fed pregnant sows without affecting production. C1 [Sapkota, A.; Richert, B. T.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Marchant-Forde, J. N.; Lay, D. C., Jr.] USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. RP Lay, DC (reprint author), USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. EM Don.Lay@ars.usda.gov FU Pork Checkoff, National Pork Board, Des Moines, IA; Archer Daniels Midland Company, Chicago, IL FX This work was supported in part by the Pork Checkoff, National Pork Board, Des Moines, IA, and Archer Daniels Midland Company, Chicago, IL. NR 30 TC 0 Z9 0 U1 10 U2 17 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD MAY PY 2016 VL 94 IS 5 BP 2117 EP 2127 DI 10.2527/jas2015-0013 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL6XL UT WOS:000375783300034 PM 27285708 ER PT J AU Manoukis, NC Gayle, SM AF Manoukis, N. C. Gayle, S. M. TI Attraction of wild-like and colony-reared Bactrocera cucurbitae (Diptera:Tephritidae) to cuelure in the field SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE directional movement; mark-release-recapture; pheromone; tephritid ID MEDITERRANEAN FRUIT-FLIES; DORSALIS DIPTERA TEPHRITIDAE; METHYL EUGENOL; CERATITIS-CAPITATA; DACUS-CUCURBITAE; MELON FLIES; RELEASED MALES; BAITED TRAPS; FLY DIPTERA; CUE-LURE AB The attraction of wild tephritids to semiochemical-based lures is the ideal basis for trap network design in detection programmes, but in practice, mass-reared colony insects are usually used to determine trap efficiency. For Bactrocera cucurbitae Coquillett, a lower response by wild males compared with colony-derived individuals, usually used to estimate attraction parameters, could mean that the sensitivity of detection networks targeting this pest is reduced. We describe the results of mark-release-recapture experiments with wild- and colony-derived B.cucurbitae males in a grid of cuelure-baited traps within a macadamia nut orchard in Hawaii Island designed to quantify the attraction of cuelure to each fly type. For colony males, we estimate a 65% probability of capture at 27m, intermediate with previous estimates on the attraction of methyl eugenol to Bactrocera dorsalis Hendel (36m) and trimedlure to Ceratitis capitata Wiedemann (14m) at the same site. Results suggest similar response over distance by wild-derived B.cucurbitae compared with colony in the field, but there may be qualitative differences in response between wild and colony based on very low response of wild males in a standard bioassay of attraction. For both fly types, the estimates of attraction in the smaller of two grid sizes tested were lower than for the larger spacing, suggesting trap competition was a factor at an intertrap distance of 75m. Dispersal patterns within the grid were generally to the south for the colony-derived males and more variable for the wild-derived males. In neither case was the direction of recapture correlated with the prevailing direction of the wind. C1 [Manoukis, N. C.; Gayle, S. M.] USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. [Gayle, S. M.] Univ Hawaii, Trop Conservat Biol & Environm Sci Program, Dept Biol, Hilo, HI 96720 USA. RP Manoukis, NC (reprint author), USDA ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM nicholas.manoukis@ars.usda.gov FU USDA-ARS; International Atomic Energy Agency Technical Contract [18462/R0] FX We would like to thank Mike McKenney and Keith Shigetani for providing melon flies for these experiments from the DKI-US-PBARC insect colony and to Thomas Mangine, Andrew Yoshimoto, Caley Saragosa and others at DKI-US-PBARC for assistance in conducting the experiments in the field. We also are grateful to Island Princess for permission to work in their macadamia nut orchard in Keaau. We thank Roger Vargas, Grant McQuate and two anonymous reviewers for constructive comments on earlier versions of this paper. This work was funded by USDA-ARS and International Atomic Energy Agency Technical Contract No. 18462/R0. Opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. USDA is an equal opportunity provider and employer. NR 40 TC 0 Z9 0 U1 7 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD MAY PY 2016 VL 140 IS 4 BP 241 EP 249 DI 10.1111/jen.12251 PG 9 WC Entomology SC Entomology GA DJ8WO UT WOS:000374494400001 ER PT J AU Knight, AL Basoalto, E Stelinski, LL AF Knight, A. L. Basoalto, E. Stelinski, L. L. TI Variability in the efficacy of sex pheromone lures for monitoring oriental fruit moth (Lepidoptera: Tortricidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Cydia pomonella; Grapholita molesta; monitoring; peach ID GRAPHOLITA-MOLESTA LEPIDOPTERA; COMPONENT; RATES; PESTS AB Studies were conducted in Chile and the United States to compare the attractiveness of various commercial sex pheromone lures and two experimental lures for oriental fruit moth, Grapholita molesta (Busck), in peach orchards treated with or without sex pheromone dispensers. The experimental lures contained the three-component sex pheromone blend of G.molesta: Z-8-dodecenyl acetate, E-8-dodecenyl acetate and Z-8-dodecenol (Z8-12:OH), and the sex pheromone of codling moth, Cydia pomonella (L.), (E,E)-8,10-dodecadien-1-ol, (codlemone). Commercial lures varied in their substrate, initial loading and blend ratio of components. Significant differences in male catches were found among commercial lures inorchards treated with or without sex pheromone dispensers. Experimental lures with the addition of codlemone significantly increased the catches of G.molesta using lures loaded with 0%, 1% or 5% Z8-12:OH in the G.molesta blend compared with the same ratio of components in just the G.molesta blend. The experimental lures were significantly more attractive than all commercial lures in the untreated orchard. However, moth catch with the experimental lures in the sex pheromone-treated orchard was only intermediate among all of the lures tested. These findings highlight the need to develop more effective and standardized lures that can be used in trap-based monitoring programme for this important pest. C1 [Knight, A. L.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA USA. [Basoalto, E.] Univ Austral Chile, Fac Ciencias Agr, Inst Prod & Sanidad Vegetal, Valdivia, Chile. [Stelinski, L. L.] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL USA. RP Knight, AL (reprint author), USDA ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov NR 20 TC 0 Z9 0 U1 4 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD MAY PY 2016 VL 140 IS 4 BP 261 EP 267 DI 10.1111/jen.12253 PG 7 WC Entomology SC Entomology GA DJ8WO UT WOS:000374494400003 ER PT J AU Paul, M Phillips, JG Renye, JA AF Paul, Moushumi Phillips, John G. Renye, John A., Jr. TI Short communication: Measuring the angiotensin-converting enzyme inhibitory activity of an 8-amino acid (8mer) fragment of the C12 antihypertensive peptide SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE antihypertensive peptide; bovine milk; solid-phase peptide synthesis; 8-amino acid peptide ID BIOACTIVE PEPTIDES; CASEIN; MILK; HYDROLYSATE; PROTEINASES; BULGARICUS; BACTERIA; PRTB AB An 8-AA (8mer) fragment (PFPEVFGK) of a known antihypertensive peptide derived from bovine alpha(s1)-casein (C12 antihypertensive peptide) was synthesized by microwave-assisted solid-phase peptide synthesis and purified by reverse phase HPLC. Its ability to inhibit angiotensin-converting enzyme (ACE) was assessed and compared with that of the parent 12mer peptide (FFVAPFPEVFGK) to determine the effect of truncating the sequence on overall hypotensive activity. The activity of the truncated 8mer peptide was found to be almost 1.5 times less active than that of the 12mer, with ACE-inhibiting IC50 (half-maximal inhibitory concentration) values of 108 and 69 mu M, for the 8mer and 12mer, respectively. Although the 8mer peptide is less active than the original 12mer peptide, its overall activity is comparable to activities reported for other small proteins that elicit physiological responses within humans. These results suggest that microbial degradation of the 12mer peptide would not result in a complete loss of antihypertensive activity if used to supplement fermented foods and that the stable 8mer peptide could have potential as a blood pressure lowering agent for use in functional foods. C1 [Paul, Moushumi; Renye, John A., Jr.] ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Phillips, John G.] ARS, Off Area Director, North Atlantic Area, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Renye, JA (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM john.renye@ars.usda.gov NR 25 TC 0 Z9 0 U1 8 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2016 VL 99 IS 5 BP 3263 EP 3266 DI 10.3168/jds.2015-10437 PG 4 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DK1YR UT WOS:000374712100003 PM 26971162 ER PT J AU Brewster, JD Paul, M AF Brewster, Jeffrey D. Paul, Moushumi TI Short communication: Improved method for centrifugal recovery of bacteria from raw milk applied to sensitive real-time quantitative PCR detection of Salmonella spp. SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE raw milk; bacteria; partitioning; recovery ID ESCHERICHIA-COLI; LISTERIA-MONOCYTOGENES; CAMPYLOBACTER-JEJUNI; GRAVITY SEPARATION; SOMATIC-CELLS; BOVINE-MILK; WHOLE MILK; COW MILK; FAT; ADHESION AB Centrifugation is widely used to isolate and concentrate bacteria from dairy products before assay. We found that more than 98% of common pathogenic bacteria added to pasteurized, homogenized, or pasteurized homogenized milk were recovered in the pellet after centrifugation, whereas less than 7% were recovered from raw milk. The remaining bacteria partitioned into the cream layer of raw milk within 5 min, and half saturation of the cream layer required a bacterial load of approximately 5 x 10(8) cfu/mL. Known treatments (e.g., heat, enzymes or solvents) can disrupt cream layer binding and improve recovery from raw milk, but can also damage bacteria and compromise detection. We developed a simple, rapid agitation treatment that disrupted bacteria binding to the cream layer and provided more than 95% recovery without affecting bacteria viability. Combining this simple agitation treatment with a previously developed real-time quantitative PCR assay allowed the detection of Salmonella spp. in raw milk at 4 cfu/mL within 3 h. To our knowledge, this is the first report of an effective method for achieving high centrifugal recovery of bacteria from raw milk without impairing bacterial viability. C1 [Brewster, Jeffrey D.] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, North Atlantic Area,Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Paul, Moushumi] ARS, Dairy & Funct Foods Res Unit, USDA, North Atlantic Area,Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Brewster, JD (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, North Atlantic Area,Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM jeffrey.brewster@ars.usda.gov FU USDA-ARS Project [8072-42000-071-00] FX The authors acknowledge David Barbano (Department of Food Science, Cornell University, Ithaca, NY) for his valuable comments and advice, and Raymond Kwoczak (USDA-ARS-NAA-ERRC) for technical assistance. This research was funded under USDA-ARS Project no. 8072-42000-071-00. NR 18 TC 0 Z9 0 U1 7 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2016 VL 99 IS 5 BP 3375 EP 3379 DI 10.3168/jds.2015-9655 PG 5 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DK1YR UT WOS:000374712100016 PM 26971150 ER PT J AU Coblentz, WK Coffey, KP Chow, EA AF Coblentz, W. K. Coffey, K. P. Chow, E. A. TI Storage characteristics, nutritive value, and fermentation characteristics of alfalfa packaged in large-round bales and wrapped in stretch film after extended time delays SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE alfalfa; baled silage; oxygen barrier; wrapping delay ID SITU DISAPPEARANCE KINETICS; NEUTRAL DETERGENT FIBER; ORCHARDGRASS HAYS; SILAGE; PRESERVATION; QUALITY; FORAGES; DIGESTIBILITY; CONSERVATION; RYEGRASS AB The production of baled silage is attractive to producers because it offers advantages over dry hay, particularly by limiting risks associated with wet or unstable weather conditions. Our objectives were to test the effects of delayed wrapping on silage fermentation, storage characteristics, and the nutritive value of baled alfalfa silages. To accomplish this, large-round bales of alfalfa were wrapped in plastic film within 4 h of baling (d 0), or after delays of 1, 2, or 3 d. A secondary objective was to evaluate a prototype bale wrap containing an O-2-limiting barrier (OB) against an identical polyethylene wrap without the O-2 barrier (SUN). Sixty-four 1.19 x 1.25-m bales of alfalfa were made from 4 field blocks at a mean moisture concentration of 59.1 +/- 4.3% with a mean initial wet bale weight of 473 +/- 26.4 kg. Two bales per field block were assigned to each combination of bale wrap (SUN or OB) and wrapping time (0, 1, 2, or 3 d postbaling), and one bale of each pair was fitted with a thermocouple placed in the geometric center of each bale. All bales were sampled after a 97-d storage period. Internal bale temperatures, recorded at the time bales were wrapped, were greater for all bales with wrapping delays compared with bales wrapped on d 0 (54.9 vs. 34.9 degrees C), and increased to a maximum of 63.9 degrees C after a 3-d delay exhibiting a linear effect of time delay. Total silage fermentation acids (lactic, acetic, propionic, butyric, and isobutyric) were greatest when bales were wrapped on d 0 compared with all bales wrapped with time delays (4.64 vs. 2.26% of DM), and declined with linear, and quadratic effects of wrapping delay. Total fermentation acids also were related quadratically to internal bale temperature by regression [y (% of DM) = 0.0042x(2) - 0.50x 17.1; R-2 = 0.725]. Similar responses were observed for lactic acid, except that trends were linear, both for orthogonal contrasts evaluating length of wrapping delay, and in regressions on internal bale temperature [y (% of DM) = 0.046x + 3.5; R-2 = 0.663]. Butyric acid also was detected, regardless of treatment, but was greatest within bales wrapped on d 0 compared with those with wrapping delays (0.99 vs. 0.38% of DM), and a similar response (0.68 vs. 0.52% of DM) was observed for NH3-N, suggesting that clostridial activity occurred during silage fermentation. Based on these results, silage fermentation characteristics and the nutritive value declined with time delays before wrapping, but responses were exacerbated when delays exceeded. C1 [Coblentz, W. K.] USDA ARS, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. [Coffey, K. P.] Univ Arkansas, Dept Anim Sci, Fayetteville, AR 72701 USA. [Chow, E. A.] Kuraray Amer Inc, Pasadena, TX 77507 USA. RP Coblentz, WK (reprint author), USDA ARS, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. EM wayne.coblentz@ars.usda.gov FU Kuraray America Inc. [58-3655-2-512]; USDA-ARS CRIS funds FX Research was supported in part by a generous grant (Agreement #58-3655-2-512) from Kuraray America Inc., and by appropriated USDA-ARS CRIS funds. NR 32 TC 0 Z9 0 U1 9 U2 23 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2016 VL 99 IS 5 BP 3497 EP 3511 DI 10.3168/jds.2015-10380 PG 15 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DK1YR UT WOS:000374712100026 PM 26923055 ER PT J AU Garcia, M Elsasser, TH Juengst, L Qu, Y Bequette, BJ Moyes, KM AF Garcia, M. Elsasser, T. H. Juengst, L. Qu, Y. Bequette, B. J. Moyes, K. M. TI Short communication: Amino acid supplementation and stage of lactation alter apparent utilization of nutrients by blood neutrophils from lactating dairy cows in vitro SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE amino acids; inflammation; lactating cows; neutrophils ID GENE-EXPRESSION; TRANSITION PERIOD; IMMUNE-RESPONSE; DISEASES; ARGININE; LIPOPOLYSACCHARIDE; PERFORMANCE; GLUTAMINE; GLUCOSE; HEALTH AB Glutamine is the preferred AA used by polymorphonuclear leukocytes (PMN) during the inflammatory response. However, the effect of other AA on bovine PMN response during inflammation and how this is altered by stage of lactation has not been fully elucidated. The objective of this study was to determine the effect of additional AA supplementation (pool of AA excluding Gln), on AA profiles, gene expression, and inflammatory function of PMN from dairy cows in early and mid lactation in vitro. We used 18 Holstein cows for this study. Polymorphonuclear leukocytes were isolated. Working solutions of AA (0 or 4 mM) and LPS (0 or 50 mu g/mL) were added to cell populations suspended in RPMI and incubated for 2 h at 37 degrees C. We used a subset of samples for gene and protein expression. Concentrations of AA in medium were determined using gas chromatography-mass spectrometry with norleucine as an internal standard. Apparent AA and glucose utilization were calculated by subtracting the concentration after from that of before incubation. Data were analyzed as a randomized block design. Challenge with LPS increased the expression of proinflammatory genes and AA supplementation decreased both the expression of some proinflammatory genes and the media concentrations of tumor necrosis factor-a. Neither stage of lactation, LPS challenge, nor AA supplementation altered the chemotactic or phagocytic abilities of PMN in vitro. Polymorphonuclear leukocytes supplemented with AA had greater concentrations and apparent utilization of most of the supplemented AA, whereas the unsupplemented group had greater apparent utilization of glucose. Alanine was not provided in the media but was present in spent media, and Ile, Gly, and Pro were greater in spent media than in media before incubation indicating synthesis of these AA. Regarding expression of genes involved in nutrient metabolism, the expression of G6PD, coding for the enzyme glucose 6-phosphate dehydrogenase, was increased and that of PDHA1, coding for the enzyme pyruvate dehydrogenase a 1, tended to increase with AA supplementation. Due to the lower concentration of tumor necrosis factor-a in media coupled with a downregulation of several proinflammatory genes, we concluded that AA, rather than Gln, alter the inflammatory response of bovine blood PMN. Independent from Gln, blood PMN from cows in early lactation may use certain AA as their primary carbon source for energy than cows in later lactation. Evaluating cows during the early postpartum period will provide additional information on the effect of stage of lactation and nutrient supplementation on PMN function. C1 [Garcia, M.; Juengst, L.; Qu, Y.; Bequette, B. J.; Moyes, K. M.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20740 USA. [Elsasser, T. H.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. RP Moyes, KM (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20740 USA. EM kmoyes@umd.edu FU University of Maryland ADVANCE Competitive Grants Program; Maryland Agricultural Experiment Station FX Funding for this study was from the University of Maryland ADVANCE Competitive Grants Program and the Maryland Agricultural Experiment Station. The authors acknowledge the USDA-ARS staff in Beltsville, Maryland, for their assistance with animal care and data collection. NR 26 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2016 VL 99 IS 5 BP 3777 EP 3783 DI 10.3168/jds.2015-10086 PG 7 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DK1YR UT WOS:000374712100052 PM 26971158 ER PT J AU Manzanilla-Pech, CIV Veerkamp, RF Tempelman, RJ van Pelt, ML Weigel, KA VandeHaar, M Lawlor, TJ Spurlock, DM Armentano, LE Connor, EE Staples, CR Hanigan, M De Haas, Y AF Manzanilla-Pech, C. I. V. Veerkamp, R. F. Tempelman, R. J. van Pelt, M. L. Weigel, K. A. VandeHaar, M. Lawlor, T. J. Spurlock, D. M. Armentano, L. E. Connor, E. E. Staples, C. R. Hanigan, M. De Haas, Y. TI Genetic parameters between feed-intake-related traits and conformation in 2 separate dairy populations-the Netherlands and United States (vol 99, pg 443, 2016) SO JOURNAL OF DAIRY SCIENCE LA English DT Correction C1 [Connor, E. E.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD USA. NR 1 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2016 VL 99 IS 5 BP 4095 EP 4095 DI 10.3168/jds.2016-99-5-4095 PG 1 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DK1YR UT WOS:000374712100081 ER PT J AU Amatya, DM Harrison, CA AF Amatya, Devendra M. Harrison, Charles A. TI Grass and Forest Potential Evapotranspiration Comparison Using Five Methods in the Atlantic Coastal Plain SO JOURNAL OF HYDROLOGIC ENGINEERING LA English DT Article DE Penman-Monteith; Priestley-Taylor; Turc; Thornthwaite; Hargreaves-Samani; Leaf area index; Stomatal conductance; Santee experimental forest ID STOMATAL CONDUCTANCE; DRAINMOD-FOREST; PINE PLANTATION; PENMAN-MONTEITH; SOUTH-CAROLINA; NORTH-CAROLINA; REFERENCE CROP; CLIMATE-CHANGE; RIVER-BASIN; MODELS AB Studies examining potential evapotranspiration (PET) for a mature forest reference compared with standard grass are limited in the current literature. Data from three long-term weather stations located within 10 km of each other in the USDA Forest Service Santee Experimental Forest (SEF) in coastal South Carolina were used to (1) evaluate monthly and annual PET estimates from five different methods with varying complexities [Penman-Monteith (P-M), Turc, Thornthwaite (Thorn), Priestley-Taylor (P-T), and Hargreaves-Samani (H-S)] at two grass reference sites; and (2) compare results for the grass sites with PET estimated using the P-M method for a forest reference site using measured daily climatic data for the 2011-2014 period. The grass reference sites are located at the SEF headquarters (SHQ) and in the Turkey Creek watershed (TC). The forest reference station is on a 27-m-tall tower above the canopy of a pine/mixed hardwood forest in watershed WS80 in the SEF. At the WS80 forest site, the highest annual PET (1,351 mm) was observed in 2011 with the lowest rainfall (934 mm), and the lowest PET (1,017 mm) was observed in 2013 with the highest rainfall (1,433 mm), which is consistent with the two grass sites. The temperature-based H-S method yielded estimated monthly and annual PETs that were in better agreement than those of another temperature-based Thorn method at both grass sites when compared against the P-M PET for the forest site. The P-M-based PET values estimated for the SHQ grass site were significantly lower (alpha = 0.05) than those obtained at the TC grass site and the P-M PET values for the WS80 forest site. The solar radiation-based Turc and temperature-based Thorn PET estimates at both grass sites were significantly different (alpha = 0.05) from the P-M PET estimates for the forest. These results for the grass sites demonstrate that PET estimates are sensitive to the method used, resulting in significantly different estimates using a single method even for nearby sites because of differences in the complexity of describing the PET process, climatic factors, and interaction with site vegetation types. When compared with the P-M PET for the forest site, the P-T method was in the closest agreement, with the highest R-2 of 0.96 and the least bias of 9.7% in mean monthly estimates, followed by the temperature-based H-S with an R-2 of 0.95 and a bias of 12.6% at the SHQ grass site. It is concluded that the simpler P-T and H-S methods appear to be adequate to estimate forest P-M PET and that their estimates are within the error bounds of the data-intensive P-M PET method for coastal forests. (C) 2016 American Society of Civil Engineers. C1 [Amatya, Devendra M.; Harrison, Charles A.] USDA, Forest Serv, Ctr Forested Wetlands Res, 3734 Highway 402, Cordesville, SC 29434 USA. RP Amatya, DM (reprint author), USDA, Forest Serv, Ctr Forested Wetlands Res, 3734 Highway 402, Cordesville, SC 29434 USA. EM damatya@fs.fed.us NR 56 TC 1 Z9 1 U1 4 U2 15 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 1084-0699 EI 1943-5584 J9 J HYDROL ENG JI J. Hydrol. Eng. PD MAY PY 2016 VL 21 IS 5 AR 05016007 DI 10.1061/(ASCE)HE.1943-5584.0001341 PG 13 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DK2QG UT WOS:000374758900006 ER PT J AU Boucias, D Liu, S Meagher, R Baniszewski, J AF Boucias, D. Liu, S. Meagher, R. Baniszewski, J. TI Fungal dimorphism in the entomopathogenic fungus Metarhizium rileyi: Detection of an in vivo quorum-sensing system SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Metarhizium rileyi; Dimorphism; Quorum sensing molecules; QS activity; Mycopathogens; Pathogenesis ID HYPHOMYCETE NOMURAEA-RILEYI; SPODOPTERA-EXIGUA LARVAE; CANDIDA-ALBICANS; BEAUVERIA-BASSIANA; FARNESOL; HEMOLYMPH; VIRULENCE; CUTICLE; MORPHOGENESIS; TYROSOL AB This investigation documents the expression of the in vivo dimorphic program exhibited by the insect mycopathogen Metarhizium rileyi. This insect mycopathogen represents the key mortality factor regulating various caterpillar populations in legumes, including subtropical and tropical soybeans. Using two hosts and M. rileyi isolates, we have measured M. rileyi growth rates under in vivo and in vitro conditions and have assessed the pathogen's impact on host fitness. Significantly, the hyphal bodies-to-mycelia transition that occurs at the late infection stage is regulated by a quorum-sensing molecule(s) (QSM) that triggers hyphal bodies (Hb) to synchronously switch to the tissue-invasive mycelia. Within hours of this transition, the host insect succumbs to mycosis. The production of the QS chemical(s) occurs when a quorum of Hb is produced in the hemolymph (late-stage infection). Furthermore, the QS activity detected in late-stage infected sera is unique and is unrelated to any known fungal QSM. The lack of similar QS activity from conditioned media of M. rileyi suggests that the chemical signal(s) that mediates the dimorphic switch is produced by host tissues in response to a quorum of hyphal bodies produced in the host hemolymph. The serum-based QS activity is retained after lyophilization, mild heat treatment, and proteinase digestion. However, attempts to extract/identify the QSM have been unsuccessful. Results suggest that the observed hyphal body-to-mycelia transition is a multi-step process involving more than one chemical signal. (C) 2016 Elsevier Inc. All rights reserved. C1 [Boucias, D.; Baniszewski, J.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Liu, S.] Liaocheng Univ, Coll Agr, Liaocheng, Shandong, Peoples R China. [Meagher, R.] USDA ARS CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. RP Boucias, D (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. EM pathos@ufl.edu FU International Cooperation Program for Excellent Lecturers of Shandong Provincial Education Department; Natural Science Foundation of Shandong Province, China [ZR2014CL023] FX The authors thank Jane Medley (Univ. Florida) for assistance in preparing the graphics used in supplemental figures. Shouzou Liu received support from the International Cooperation Program for Excellent Lecturers (2012) of Shandong Provincial Education Department and from the Natural Science Foundation of Shandong Province (ZR2014CL023), China. NR 45 TC 2 Z9 2 U1 4 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2016 VL 136 BP 100 EP 108 DI 10.1016/j.jip.2016.03.013 PG 9 WC Zoology SC Zoology GA DL4SK UT WOS:000375627300016 PM 27018146 ER PT J AU Montalva, C dos Santos, K Collier, K Rocha, LFN Fernandes, EKK Castrillo, LA Luz, C Humber, RA AF Montalva, Cristian dos Santos, Karine Collier, Karin Rocha, Luiz F. N. Fernandes, Everton K. K. Castrillo, Louela A. Luz, Christian Humber, Richard A. TI First report of Leptolegnia chapmanii (Peronosporomycetes: Saprolegniales) affecting mosquitoes in central Brazil SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Pathogen; Watermold; Distribution; Lectotype; Epitype; Culicidae; Aedes ID AQUATIC FUNGUS-LEPTOLEGNIA; LARVAE DIPTERA; AEDES-AEGYPTI; HOST-RANGE; INFECTION; CULICIDAE; PATHOGEN AB Numerous isolates of an oomycete 'fungus', Leptolegnia chapmanii, are reported from Brazil for the first time. This aquatic pathogen was baited with Aedes aegypti sentinel larvae from stagnant, temporary bodies of water in selected locations under secondary tropical forest in and near the central Brazilian city of Goiania and from more distant sites in the western and northern regions of the state of Goias. Isolates were identified based on their morphological and developmental characters, comparative sequence data for the ITS and TEF loci, as well as their rapid activity against A. aegypti larvae. Taxonomic issues affecting the application of the name L. chapmanii and its typification are rectified. This study contributes to a better understanding of the presence and distribution of this oomycete in Brazil, its sequence-based identification, and of its potential as a biological agent against mosquito vectors. Published by Elsevier Inc. C1 [Montalva, Cristian; dos Santos, Karine; Rocha, Luiz F. N.; Fernandes, Everton K. K.; Luz, Christian] Univ Fed Goias, Lab Patol Invertebrados, Inst Patol Trop & Saude Publ, Goiania, Go, Brazil. [Collier, Karin] Ctr Univ Gurupi UnirG, Gurupi, Tocantins, Brazil. [Rocha, Luiz F. N.] Inst Fed Educ Ciencia & Tecnol Goias IFG, Aparecida De Goiania, Go, Brazil. [Castrillo, Louela A.] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Humber, Richard A.] USDA ARS, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. RP Humber, RA (reprint author), USDA ARS, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM richard.humber@ars.usda.gov RI Fernandes, Everton/I-4890-2012; OI Fernandes, Everton/0000-0001-7062-3295; Humber, Richard/0000-0002-1760-3030 FU CAPES Coordination of the Improvement of Higher Education; CSF [PVE 71/2013, 149988]; Instituto Federal de Educacao, Ciencia e Tecnologia de Goias [IFG-PROAPP 006/2014] FX The authors thank Welington T. Rocha (Secretaria Municipal de Saude, Goiania, GO), Ademar Delfino Pires (Aruana, GO), Juscelino Rodrigues Filho, Stefi Buchter (LPI-IPTSP, Goiania), Andre Lunardelli (Santa Branca Ecoturismo, Terezopolis, GO), Fabio Padula (Reserva Bacupari, Cavalcante, GO), who have provided material assistance in the collection of the Brazilian isolates discussed here. Special thanks are due to John V. Freudenstein (Curator, The Ohio State University Herbarium) for his generous assistance in attempting to locate Seymour's collections of L. chapmanii; to Drs. Jeffrey C. Lord (retired), Claudia C. Lopez Lastra (CEPAVE, La Plata, Argentina), and Mark A. Spencer (Natural History Museum, London) for information about the L. chapmanii isolates studied by Dick et al. (1999). This study was supported by CAPES Coordination of the Improvement of Higher Education, CSF, PVE 71/2013, 149988 (to CL and RAH) and Instituto Federal de Educacao, Ciencia e Tecnologia de Goias, IFG-PROAPP 006/2014 (to LFNR). NR 26 TC 0 Z9 0 U1 1 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2016 VL 136 BP 109 EP 116 DI 10.1016/j.jip.2016.03.012 PG 8 WC Zoology SC Zoology GA DL4SK UT WOS:000375627300017 PM 27018147 ER PT J AU Ding, WF Zhang, XC AF Ding Wen-feng Zhang Xun-chang TI An evaluation on using soil aggregate stability as the indicator of interrill erodibility SO JOURNAL OF MOUNTAIN SCIENCE LA English DT Article DE Soil erodibility; Interrill; Aggregate stability; Soil erosion ID FRACTAL DIMENSION; SIZE DISTRIBUTIONS; LOAMY SOILS; EROSION; RUNOFF; INDEX; FRAGMENTATION; INFILTRATION; INTENSITY; NITROGEN AB Aggregate stability is a very important predictor of soil structure and strength, which influences soil erodibility. Several aggregate stability indices were selected for estimating interrill erodibility of four soil types with contrasting properties from temperate and subtropical regions of China. This study was conducted to investigate how closely the soil interrill erodibility factor in the Water Erosion Prediction Project (WEPP) model relates to soil aggregate stability. The mass fractal dimension (FD), geometric mean diameter (GMD), mean weight diameter (MWD), and aggregate stability index (ASI) of soil aggregates were calculated. A rainfall simulator with a drainable flume (3.0 m long x 1.0 m wide x 0.5 m deep) was used at four slope gradients (5A degrees, 10A degrees,15A degrees and 20A degrees), and four rainfall intensities (0.6, 1.1, 1.7 and 2.5 mm/min). Results indicated that the interrill erodibility (Ki) values were significantly correlated to the indices of ASI, MWD, GMD, and FD computed from the aggregate wet-sieve data. The K (i) had a strong positive correlation with FD, as well as a strong negative correlation with ASI, GMD, and MWD. Soils with a higher aggregate stability and lower fractal dimension have smaller K (i) values. Stable soils were characterized by a high percentage of large aggregates and the erodible soils by a high percentage of smaller aggregates. The correlation coefficients of K (i) with ASI and GMD were greater than those with FD and MWD, implying that both the ASI and GMD may be better alternative parameters for empirically predicting the soil K (i) factor. ASI and GMD are more reasonable in interrill soil erodibility estimation, compared with K (i) calculation in original WEPP model equation. Results demonstrate the validation of soil aggregation characterization as an appropriate indicator of soil susceptibility to erosion in contrasting soil types in China. C1 [Ding Wen-feng] Yangtze River Sci Res Inst, Soil & Water Conservat Dept, Wuhan 430010, Peoples R China. [Zhang Xun-chang] USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. RP Ding, WF (reprint author), Yangtze River Sci Res Inst, Soil & Water Conservat Dept, Wuhan 430010, Peoples R China. EM dingwf@mail.crsri.cn; jzhang@grl.ars.usda.gov FU National Natural Science Foundation of China [41271303, 40901135]; National Key Technology RD Program [2012BAK10B04, 2008BAD98B02]; Non-profit Industry Financial Program of MWR [201301058]; Changjiang River Scientific Research Institute of Sciences Innovation Team Project [CKSF2012052 / TB]; Central public welfare scientific research project [CKSF2013013/TB] FX This work was supported by the National Natural Science Foundation of China (Grant Nos. 41271303, 40901135), the National Key Technology R&D Program (Grant Nos. 2012BAK10B04, 2008BAD98B02), the Non-profit Industry Financial Program of MWR (Grant No. 201301058), and the Changjiang River Scientific Research Institute of Sciences Innovation Team Project (Grant No. CKSF2012052 / TB), Central public welfare scientific research project (Grant No. CKSF2013013/TB). NR 61 TC 1 Z9 1 U1 8 U2 18 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1672-6316 EI 1993-0321 J9 J MT SCI-ENGL JI J Mt. Sci. PD MAY PY 2016 VL 13 IS 5 BP 831 EP 843 DI 10.1007/s11629-015-3447-4 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DL7AI UT WOS:000375790800007 ER PT J AU Cullen, KW Liu, Y Thompson, DI AF Cullen, Karen W. Liu, Yan Thompson, Debbe I. TI Meal-Specific Dietary Changes From Squires Quest! II: A Serious Video Game Intervention SO JOURNAL OF NUTRITION EDUCATION AND BEHAVIOR LA English DT Article DE video game; fruit; vegetables; children; implementation intentions ID POWER PLUS PROGRAM; FOOD-DUDES PROGRAM; VEGETABLE CONSUMPTION; IMPLEMENTATION INTENTIONS; CHILDRENS FRUIT; 4TH-GRADE; METAANALYSIS; OVERWEIGHT; CHILDHOOD; ADULTHOOD AB Objective: Squire's Quest! II: Saving the Kingdom of Fivealot, an online video game, promotes fruit and vegetable (FV) consumption. An evaluation study varied the type of implementation intentions used during the goal-setting process (none, action, coping, or both action and coping plans). Participants who created action plans reported higher FV consumption 6 months after baseline. This study assessed changes by specific meal in that study. Methods: A total of 400 fourth-and fifth-grade children completed 3 24-hour recalls at baseline and 6 months later. These were averaged to obtain FV intake. Analyses used repeated-measures ANCOVA. Results: There was a significant group by time effect for vegetables at 6 months (P = .01); Action (P =.01) and coping (P =.04) group participants reported higher vegetable intake at dinner. There were significant increases in fruit intake at breakfast (P =.009), lunch (P =.01), and snack (P <.001). Conclusions and Implications: Setting meal-specific goals and action or coping plans may enable children to overcome barriers and consume FV. C1 [Cullen, Karen W.; Liu, Yan; Thompson, Debbe I.] Baylor Coll Med, USDA, Agr Res Ctr, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Cullen, KW (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM kcullen@bcm.edu FU National Institutes of Health, National Institute of Child Health and Human Development [HD050595]; USDA/ARS [58-6250-0-008] FX This work is a publication of the US Department of Agriculture/Agricultural Research Center (USDA/ARS), Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX. This project was supported by the National Institutes of Health, National Institute of Child Health and Human Development Grant No. HD050595 (to Dr Thompson). This work is also a publication of the USDA/ARS, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and was funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-0-008. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US Government. NR 34 TC 3 Z9 3 U1 4 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1499-4046 EI 1878-2620 J9 J NUTR EDUC BEHAV JI J. Nutr. Educ. Behav. PD MAY PY 2016 VL 48 IS 5 BP 326 EP + DI 10.1016/j.jneb.2016.02.004 PG 6 WC Education, Scientific Disciplines; Nutrition & Dietetics SC Education & Educational Research; Nutrition & Dietetics GA DL4DQ UT WOS:000375584100007 PM 27169641 ER PT J AU Zhou, SP Okekeogbu, I Sangireddy, S Ye, ZJ Li, H Bhatti, S Hui, DF McDonald, DW Yang, Y Shree Giri Howe, KJ Fish, T Thannhauser, TW AF Zhou, Suping Okekeogbu, Ikenna Sangireddy, Sasikiran Ye, Zhujia Li, Hui Bhatti, Sarabjit Hui, Dafeng McDonald, Daniel W. Yang, Yong Shree Giri Howe, Kevin J. Fish, Tara Thannhauser, Theodore W. TI Proteome Modification in Tomato Plants upon Long-Term Aluminum Treatment SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE iTRAQ proteomics; tomato; roots; leaves; embryos; seed coat; functional pathways; aluminum stress ID ROOT-GROWTH INHIBITION; TRANSCRIPTION FACTORS; ESCHERICHIA-COLI; ACID SOILS; HIGH-TEMPERATURE; AL TOXICITY; LOW PH; TOLERANCE; ARABIDOPSIS; TRANSPORTER AB This study aimed to identify the aluminum (Al)-induced proteomes in tomato (Solanum lycopersicum, "Micro Tom") after long-term exposure to the stress factor. Plants were treated in Magnavaca's solution (pH 4.5) supplemented with 7.5 mu M Al3+ ion activity over a 4 month period beginning at the emergence of flower buds and ending when the lower mature leaves started to turn yellow. Proteomes were identified using a 8-plex isobaric tags for relative and absolute quantification (iTRAQ) labeling strategy followed by a two-dimensional (high- and low-pH) chromatographic separation and final generation of tandem mass spectrometry (MS/MS) spectra of tryptic peptides on an LTQ:Orbitrap Elite mass spectrometer. Principal component analysis revealed that the Al-treatment had induced systemic alterations in the proteomes from roots and leaves but not seed tissues. The significantly changed root proteins were shown to have putative functions in Al3+ ion uptake and transportation, root development, and a multitude of other cellular processes. Changes in the leaf proteome indicate that the light reaction centers of photosynthetic machinery are the primary targets of Al-induced stress. Embryo and seed-coat tissues derived from Al-treated plants were enriched with stress proteins. The biological processes involving these Al-induced proteins concur with the physiological and morphological changes, such as the disturbance of mineral homeostasis (higher contents of Al, P, and Fe and reduced contents of S, Zn, and Mn in Al-treated compared to nontreated plants) in roots and smaller sizes of roots and the whole plants. More importantly, the identified significant proteins might represent a molecular mechanism for plants to develop toward establishing the Al tolerance and adaptation mechanism over a long period of stress treatment. C1 [Zhou, Suping; Okekeogbu, Ikenna; Sangireddy, Sasikiran; Ye, Zhujia; Li, Hui; Bhatti, Sarabjit; Hui, Dafeng] Tennessee State Univ, Coll Agr Human & Nat Sci, Dept Agr & Environm Sci, 3500 John A Merritt Blvd, Nashville, TN 37209 USA. [McDonald, Daniel W.] Phenotype Screening Corp, 4028 Papermill Rd, Knoxville, TN 37909 USA. [Yang, Yong; Shree Giri; Howe, Kevin J.; Fish, Tara; Thannhauser, Theodore W.] USDA ARS, RW Holley Ctr Agr & Hlth, Plant Soil & Nutr Res Unit, Tower Rd, Ithaca, NY 14853 USA. RP Zhou, SP (reprint author), Tennessee State Univ, Coll Agr Human & Nat Sci, Dept Agr & Environm Sci, 3500 John A Merritt Blvd, Nashville, TN 37209 USA.; Thannhauser, TW (reprint author), USDA ARS, RW Holley Ctr Agr & Hlth, Plant Soil & Nutr Res Unit, Tower Rd, Ithaca, NY 14853 USA. EM zsuping@tnstate.edu; tt34@cornell.edu FU U.S. Department of Agriculture [2010-65114-20405]; U.S. Department of Agriculture Agricultural Research Service [1907-21000-036/037-00D] FX The authors thank Sheng Zhang of the Proteomics and Mass Spectrometry Facility of the Cornell University Institute of Biotechnology for expert technical assistance and helpful discussions. The authors also acknowledge Dr. Michael A. Rutzke for providing technical assistance in mineral analysis of tomato tissues, Mr. Jon Shaff for suggestions on aluminum treatment, and Dr. Lukas Mueller and his associate, Mr. Noe Fernandez, for suggestions on functional pathway analysis. ARS disclaimer: the mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This work was supported by the U.S. Department of Agriculture, grant no. 2010-65114-20405, and U.S. Department of Agriculture Agricultural Research Service, grant no. 1907-21000-036/037-00D. NR 80 TC 1 Z9 1 U1 7 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 EI 1535-3907 J9 J PROTEOME RES JI J. Proteome Res. PD MAY PY 2016 VL 15 IS 5 BP 1670 EP 1684 DI 10.1021/acs.jproteome.6b00128 PG 15 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA DL8KP UT WOS:000375891200026 PM 27052409 ER PT J AU Moriarty, KM Epps, CW Zielinski, WJ AF Moriarty, Katie M. Epps, Clinton W. Zielinski, William J. TI Forest thinning changes movement patterns and habitat use by Pacific marten SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE animal movement; California; marten; Martes caurina; movement; predation; risk; thinning; travel speed; vigilance ID NORTHERN SIERRA-NEVADA; AMERICAN MARTEN; PREDATION RISK; NATIONAL-PARK; PINE MARTENS; HOME-RANGE; POPULATION-DYNAMICS; RESOURCE SELECTION; LANDSCAPE PATTERN; INDUSTRIAL FOREST AB Simplifying stand structure to reduce fuel density is a high priority for forest managers; however, affects to Pacific marten (Martes caurina) movement and connectivity are unknown. We evaluated whether thinning forests to reduce fuels influenced movements of Pacific marten. We collected movement paths from 22 martens using global positioning system telemetry to evaluate habitat selection and describe movement patterns. We quantified motion variance, speed, and path sinuosity in 3 stand types that differed in structural complexity (i.e., complex [dense], simple [thinned], and open). We hypothesized marten movement would differ between stand types and predicted that 1) martens would select stand types with increased structural complexity (complex>simple>open); 2) movements would increase in complexity (sinuosity, motion variance) and decrease in speed when martens traveled through stands with increased structural complexity; 3) speeds would increase during summer, indicating increased movement during the breeding season; and 4) males would move more rapidly because of their larger home ranges. Martens traveled 0.5-27.2km/day and an average (SD) of 1.4 (0.4) km/hour. Martens selected home ranges with fewer openings compared to the study area overall. Within home ranges, martens strongly selected complex stands over simple stands and openings. Speed and movement complexity were most consistent over time and movements were more sinuous and slower in complex stand types compared with openings and simple stands. Movement was erratic and more linear in openings than in both complex and simple stands. In simple stands, movement patterns were intermediate between complex stands and openings. Females generally moved more slowly, sinuously, and less variably compared to males. Martens moved more quickly, less sinuously, and more variably during winter compared to summer. However, martens avoided stands with simplified structure, and the altered patterns of movement we observed in those stands suggested that such treatments may negatively affect the ability of martens to forage without increased risk of predation. Fuel treatments that simplify stand structure negatively affected marten movements and habitat connectivity. Given these risks, and because treating fuels is less justified in high elevation forests, the risks can be minimized by applying treatments below the elevations where martens typically occur. (c) 2016 The Wildlife Society. C1 [Moriarty, Katie M.; Epps, Clinton W.] Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA. [Zielinski, William J.] US Forest Serv, Pacific Southwest Res Stn, USDA, 1700 Bayview Dr, Arcata, CA 95521 USA. [Moriarty, Katie M.] US Forest Serv, Pacific NW Res Stn, USDA, 3625 93rd Ave SW, Olympia, WA 98512 USA. RP Moriarty, KM (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA.; Moriarty, KM (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM ktmoriarty22@gmail.com FU Lassen National Forest; Almanor Ranger District, California; OSU Laurels Block Grant; Western Section of the Wildlife Society (TWS); OSU Department of Fisheries and Wildlife; Oregon Chapter-TWS; Mountain Hardwear; Madshus Skis; OSU Fisheries and Wildlife Thomas G. Scott Fund FX We thank M. R. Williams and T. W. Frolli and the staff at Almanor Ranger District, and C. P. Dillingham, R. Martinez, and S. VanPetten for assistance with mapping management activities. We thank A. Moffett, the Epps and Betts labs, and the staff at Oregon State University (OSU) for logistics support. We appreciated assistance improving this manuscript from anonymous reviewers. Lastly, we thank our field technicians (M. A. Linnell, M. S. Delheimer, L. J. Kreiensieck, P. J. Tweedy, M. R. Adamczyk, K. R. Mansfield, B. R. Barry, B. A. Peterson, D. S. Hamilton, C. M. Wood, M. N. Dao, M. J. Cokeley), and the many volunteers on this project. Funding and field support were provided by the Lassen National Forest and Almanor Ranger District, California, OSU Laurels Block Grant, the Western Section of the Wildlife Society (TWS), the OSU Department of Fisheries and Wildlife, the Oregon Chapter-TWS, Mountain Hardwear, and Madshus Skis. Publication fees were supported by the OSU Fisheries and Wildlife Thomas G. Scott Fund. NR 112 TC 0 Z9 0 U1 16 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2016 VL 80 IS 4 BP 621 EP 633 DI 10.1002/jwmg.1060 PG 13 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DJ8TT UT WOS:000374486800004 ER PT J AU DeVault, TL Blackwell, BF Seamans, TW Belant, JL AF DeVault, Travis L. Blackwell, Bradley F. Seamans, Thomas W. Belant, Jerrold L. TI Identification of off airport interspecific avian hazards to aircraft SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE aviation hazard; bird strike; body mass; risk; wildlife-aircraft collision; wildlife hazard score; wildlife strike ID US CIVIL AIRCRAFT; BIRD STRIKES; FLIGHT BEHAVIOR; COLLISIONS; SAFETY; MANAGEMENT; AVIATION; LIGHTS; RADAR; RISK AB Understanding relative hazards of wildlife to aircraft is important for developing effective management programs that can minimize hazards from wildlife strikes. Although interspecific differences in hazard level of birds and mammals on airport properties are described, no studies have quantified hazard level of bird species or identified factors that influence hazard level when birds are struck beyond airport boundaries (e.g., during aircraft climb or approach). We used Federal Aviation Administration National Wildlife Strike Database records from 1990 through 31 May 2014 to identify bird species involved most often in collisions with aircraft beyond airport boundaries in the United States and to quantify the interspecific hazard level of those birds. We also investigated whether body mass, group size (single or multiple birds), region (Flyway), and season influenced the likelihood of aircraft damage and substantial damage when strikes occurred using binary logistic regression analysis. Canada geese (Branta canadensis; n=327), turkey vultures (Cathartes aura; 217), American robins (Turdus migratorius; 119), and mallards (Anas platyrhynchos; 107) were struck most often by aircraft beyond airport boundaries. Waterbirds (cormorants, ducks, geese, and to a lesser extent, gulls) and raptors (including vultures) were most likely to cause damage or substantial damage to aircraft when strikes occurred. Body mass was an important predictor of hazard level; group size, region, and season had lesser effects on hazard level. Management strategies to reduce bird strikes with aircraft beyond airport properties should be active throughout the year and prioritize waterbirds and raptors. Published 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [DeVault, Travis L.; Blackwell, Bradley F.; Seamans, Thomas W.] Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr,Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. [Belant, Jerrold L.] Mississippi State Univ, Carnivore Ecol Lab, Forest & Wildlife Res Ctr, Box 9690, Mississippi State, MS 39762 USA. RP DeVault, TL (reprint author), Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr,Ohio Field Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM travis.l.devault@aphis.usda.gov FU U.S. Department of Agriculture; FAA FX We thank S. E. Wright and P. R. Miller for helping us access and interpret data from the FAA National Wildlife Strike Database, and M. J. Begier and R. A. Dolbeer for many helpful discussions about wildlife hazards to aircraft. B. E. Washburn, E. Fernandez-Juricic, T. A. Messmer, and 2 anonymous reviewers provided helpful comments on the manuscript. Our research on wildlife strikes was funded by the U.S. Department of Agriculture and the FAA. Opinions expressed in these studies do not necessarily reflect current FAA policy decisions governing the control of wildlife on or near airports. NR 43 TC 0 Z9 0 U1 10 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2016 VL 80 IS 4 BP 746 EP 752 DI 10.1002/jwmg.1041 PG 7 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DJ8TT UT WOS:000374486800015 ER PT J AU Sadat, R Barca, E Masand, R Donti, TR Naini, A De Vivo, DC DiMauro, S Hanchard, NA Graham, BH AF Sadat, Roa Barca, Emanuele Masand, Ruchi Donti, Taraka R. Naini, Ali De Vivo, Darryl C. DiMauro, Salvatore Hanchard, Neil A. Graham, Brett H. TI Functional cellular analyses reveal energy metabolism defect and mitochondrial DNA depletion in a case of mitochondrial aconitase deficiency SO MOLECULAR GENETICS AND METABOLISM LA English DT Article DE Whole exome sequencing; mtDNA depletion; Neurodegenerative disease; TCA cycle; Mitochondrial aconitase deficiency ID MUTATIONS; GENE; ENCEPHALOPATHY; MAINTENANCE; VARIANTS; CYCLE AB Defects in the tricarboxylic acid cycle (TCA) are associated with a spectrum of neurological phenotypes that are often difficult to diagnose and manage. Whole-exome sequencing (WES) led to a rapid expansion of diagnostic capabilities in such disorders and facilitated a better understanding of disease pathogenesis, although functional characterization remains a bottleneck to the interpretation of potential pathological variants. We report a 2-year-old boy of Afro-Caribbean ancestry, who presented with neuromuscular symptoms without significant abnormalities on routine diagnostic evaluation. WES revealed compound heterozygous missense variants of uncertain significance in mitochondrial aconitase (ACO2), which encodes the TCA enzyme ACO2. Pathogenic variants in ACO2 have been described in a handful of families as the cause of infantile cerebellar-retinal degeneration syndrome. Using biochemical and cellular assays in patient fibroblasts, we found that ACO2 expression was quantitatively normal, but ACO2 enzyme activity was <20% of that observed in control cells. We also observed a deficiency in cellular respiration and, for the first time, demonstrate evidence of mitochondrial DNA depletion and altered expression of some TCA components and electron transport chain subunits. The observed cellular defects were completely restored with ACO2 gene rescue. Our findings demonstrate the pathogenicity of two VUS in ACO2, provide novel mechanistic insights to TCA disturbances in ACO2 deficiency, and implicate mitochondrial DNA depletion in the pathogenesis of this recently described disorder. (C) 2016 Elsevier Inc. All rights reserved. C1 [Sadat, Roa; Hanchard, Neil A.] Baylor Coll Med, Childrens Nutr Res Ctr, ARS, USDA, Houston, TX 77030 USA. [Masand, Ruchi; Donti, Taraka R.; Hanchard, Neil A.; Graham, Brett H.] Baylor Coll Med, Dept Mol & Human Genet, One Baylor Plaza T803, Houston, TX 77030 USA. [Barca, Emanuele] Univ Messina, Dept Neurosci, Messina, Italy. [Barca, Emanuele; Naini, Ali; DiMauro, Salvatore] Columbia Univ, Med Ctr, Houston Merritt Ctr, New York, NY USA. [De Vivo, Darryl C.] Columbia Univ, Med Ctr, Pediat Neurol, New York, NY USA. RP Graham, BH (reprint author), Baylor Coll Med, Dept Mol & Human Genet, One Baylor Plaza T803, Houston, TX 77030 USA. EM bgraham@bcm.edu OI Graham, Brett/0000-0001-8451-8154 FU NCI NIH HHS [P30 CA125123]; NCRR NIH HHS [S10 RR024574]; NIAID NIH HHS [P30 AI036211]; NICHD NIH HHS [P01 HD080642, 1U54HD083092, U54 HD083092]; NIGMS NIH HHS [R01 GM098387, R01GM098387, R21GM110190, R21 GM110190] NR 25 TC 2 Z9 2 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1096-7192 EI 1096-7206 J9 MOL GENET METAB JI Mol. Genet. Metab. PD MAY PY 2016 VL 118 IS 1 BP 28 EP 34 DI 10.1016/j.ymgme.2016.03.004 PG 7 WC Endocrinology & Metabolism; Genetics & Heredity; Medicine, Research & Experimental SC Endocrinology & Metabolism; Genetics & Heredity; Research & Experimental Medicine GA DL6HY UT WOS:000375741500006 PM 26992325 ER PT J AU Chen, XZ Wei, XH Sun, G Zhou, P Zhou, GY AF Chen, Xiuzhi Wei, Xiaohua Sun, Ge Zhou, Ping Zhou, Guoyi TI Reply to 'Space-time asymmetry undermines water yield assessment' SO NATURE COMMUNICATIONS LA English DT Letter C1 [Chen, Xiuzhi; Zhou, Guoyi] Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China. [Wei, Xiaohua] Univ British Columbia, Earth & Environm Sci & Phys Geog, Okanagan Campus, Kelowna, BC V1V 1V7, Canada. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC 27606 USA. [Zhou, Ping] Guangdong Acad Forestry, Dept Forest Ecol, Guangzhou 510520, Guangdong, Peoples R China. RP Zhou, GY (reprint author), Chinese Acad Sci, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China. EM gyzhou@scib.ac.cn NR 7 TC 0 Z9 0 U1 6 U2 11 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAY PY 2016 VL 7 AR 11604 DI 10.1038/ncomms11604 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL4ME UT WOS:000375610100001 PM 27176655 ER PT J AU Larson, JE Sheley, RL Hardegree, SP Doescher, PS James, JJ AF Larson, Julie E. Sheley, Roger L. Hardegree, Stuart P. Doescher, Paul S. James, Jeremy J. TI Do key dimensions of seed and seedling functional trait variation capture variation in recruitment probability? SO OECOLOGIA LA English DT Article DE Emergence; Germination; Plant functional type; Roots; Survival ID PLANT ECONOMICS SPECTRUM; RELATIVE GROWTH-RATE; PHENOTYPIC PLASTICITY; STRATEGY SCHEME; LEAF MASS; GERMINATION; GRASSLAND; COMMUNITY; SIZE; ESTABLISHMENT AB Seedling recruitment is a critical driver of population dynamics and community assembly, yet we know little about functional traits that define different recruitment strategies. For the first time, we examined whether trait relatedness across germination and seedling stages allows the identification of general recruitment strategies which share core functional attributes and also correspond to recruitment outcomes in applied settings. We measured six seed and eight seedling traits (lab- and field-collected, respectively) for 47 varieties of dryland grasses and used principal component analysis (PCA) and cluster analysis to identify major dimensions of trait variation and to isolate trait-based recruitment groups, respectively. PCA highlighted some links between seed and seedling traits, suggesting that relative growth rate and root elongation rate are simultaneously but independently associated with seed mass and initial root mass (first axis), and with leaf dry matter content, specific leaf area, coleoptile tissue density and germination rate (second axis). Third and fourth axes captured separate tradeoffs between hydrothermal time and base water potential for germination, and between specific root length and root mass ratio, respectively. Cluster analysis separated six recruitment types along dimensions of germination and growth rates, but classifications did not correspond to patterns of germination, emergence or recruitment in the field under either of two watering treatments. Thus, while we have begun to identify major threads of functional variation across seed and seedling stages, our understanding of how this variation influences demographic processes-particularly germination and emergence-remains a key gap in functional ecology. C1 [Larson, Julie E.] Oregon State Univ, Environm Sci Grad Program, Corvallis, OR 97330 USA. [Sheley, Roger L.] USDA ARS, Burns, OR 97720 USA. [Hardegree, Stuart P.] USDA ARS, Boise, ID 83712 USA. [Doescher, Paul S.] Oregon State Univ, Coll Forestry, Corvallis, OR 97330 USA. [James, Jeremy J.] Univ Calif Browns Valley, Div Agr & Nat Resources, Sierra Foothills Res & Extens Ctr, Browns Valley, CA 95918 USA. [Larson, Julie E.] Chapman Univ, Schmid Coll Sci & Technol, Orange, CA 92866 USA. RP Larson, JE (reprint author), Oregon State Univ, Environm Sci Grad Program, Corvallis, OR 97330 USA.; Larson, JE (reprint author), Chapman Univ, Schmid Coll Sci & Technol, Orange, CA 92866 USA. EM jlarson@chapman.edu FU USDA-Agricultural Research Service Area-wide Ecologically-based Invasive Plant Management of Annual Grasses program FX This study was funded as part of the USDA-Agricultural Research Service Area-wide Ecologically-based Invasive Plant Management of Annual Grasses program. NR 71 TC 4 Z9 4 U1 25 U2 48 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD MAY PY 2016 VL 181 IS 1 BP 39 EP 53 DI 10.1007/s00442-015-3430-3 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DJ9WM UT WOS:000374564000004 PM 26337610 ER PT J AU Guzman-Lopez, JA Abraham-Juarez, MJ Lozano-Sotomayor, P de Folter, S Simpson, J AF Guzman-Lopez, Jose Alfredo Abraham-Juarez, Maria Jazmin Lozano-Sotomayor, Paulina de Folter, Stefan Simpson, June TI Arabidopsis thaliana gonidialess A/Zuotin related factors (GlsA/ZRF) are essential for maintenance of meristem integrity SO PLANT MOLECULAR BIOLOGY LA English DT Article DE Meristem development; Gonidialess; Zuotin related factors; Arabidopsis thaliana; Bulbil development; Agave species ID MEDIATED PLANT TRANSFORMATION; YUCCA FLAVIN MONOOXYGENASES; BULBIL FORMATION; AGAVE-TEQUILANA; FLORAL ORGANS; GENES; MORPHOGENESIS; SHOOT; ZRF1; EMBRYOGENESIS AB Observation of a differential expression pattern, including strong expression in meristematic tissue of an Agave tequilana GlsA/ZRF ortholog suggested an important role for this gene during bulbil formation and developmental changes in this species. In order to better understand this role, the two GlsA/ZFR orthologs present in the genome of Arabidopsis thaliana were functionally characterized by analyzing expression patterns, double mutant phenotypes, promoter-GUS fusions and expression of hormone related or meristem marker genes. Patterns of expression for A. thaliana show that GlsA/ZFR genes are strongly expressed in SAMs and RAMs in mature plants and developing embryos and double mutants showed multiple changes in morphology related to both SAM and RAM tissues. Typical double mutants showed stunted growth of aerial and root tissue, formation of multiple ectopic meristems and effects on cotyledons, leaves and flowers. The KNOX genes STM and BP were overexpressed in double mutants whereas CLV3, WUSCHEL and AS1 were repressed and lack of AtGlsA expression was also associated with changes in localization of auxin and cytokinin. These results suggest that GlsA/ZFR is an essential component of the machinery that maintains the integrity of SAM and RAM tissue and underline the potential to identify new genes or gene functions based on observations in non-model plants. C1 [Guzman-Lopez, Jose Alfredo; Abraham-Juarez, Maria Jazmin; Simpson, June] CINVESTAV, Unidad Irapuato, Dept Plant Genet Engn, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Guanajuato 36821, Mexico. [Lozano-Sotomayor, Paulina; de Folter, Stefan] CINVESTAV Irapuato, Lab Nacl Genom Biodiversidad LANGEBIO, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Guanajuato 36821, Mexico. [Abraham-Juarez, Maria Jazmin] Univ Calif Berkeley, Ctr Plant Gene Express, USDA ARS, 800 Buchanan St, Albany, CA 94710 USA. RP Simpson, J (reprint author), CINVESTAV, Unidad Irapuato, Dept Plant Genet Engn, Km 9-6 Libramiento Norte Carretera Irapuato Leon, Guanajuato 36821, Mexico. EM jsimpson@ira.cinvestav.mx RI Simpson, June/I-1057-2012; de Folter, Stefan/E-9373-2013 OI Simpson, June/0000-0002-1891-4914; de Folter, Stefan/0000-0003-4363-7274 FU Mexican National Council of Science and Technology (CONACyT) [219888, 219883]; CONACyT [132160] FX We are very grateful to Sarah Hake for critical reading of the manuscript and provision of experimental assistance and material in histological experiments. The STM:: GUS line was kindly provided by Stewart Gillmor (Laboratorio Nacional de Genomica para la Biodiversidad LANGEBIO, CINVESTAV Irapuato). We also acknowledge the Arabidopsis Biological Resource Center and Marcos Castellanos (Nottingham Arabidopsis Stock Centre, University of Nottingham, Loughborough, UK) for provision of mutant seeds. J. A. G. L. and P. L. S. were supported by Mexican National Council of Science and Technology (CONACyT) fellowships (numbers 219888, and 219883, respectively). This work was carried out with financial support from CONACyT Grant No. 132160. We are grateful to Katia Gil Vega for technical assistance. NR 36 TC 0 Z9 0 U1 5 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4412 EI 1573-5028 J9 PLANT MOL BIOL JI Plant Mol.Biol. PD MAY PY 2016 VL 91 IS 1-2 BP 37 EP 51 DI 10.1007/s11103-016-0439-x PG 15 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DK1NN UT WOS:000374679700003 PM 26826012 ER PT J AU Gaxiola, RA Regmi, K Hirschi, KD AF Gaxiola, Roberto A. Regmi, Kamesh Hirschi, Kendal D. TI Moving On Up: H+-PPase Mediated Crop Improvement SO TRENDS IN BIOTECHNOLOGY LA English DT Editorial Material ID PROTON-PUMPING PYROPHOSPHATASE; TRANSLOCATING PYROPHOSPHATASE; SALT TOLERANCE; ARABIDOPSIS; PHLOEM; WHEAT AB Upregulation of H+-PPase in diverse crop systems triggers agriculturally beneficial phenotypes including augmented stress tolerance, improved water and nutrient use efficiencies, and increased biomass and yield. We argue that further research is warranted to maximize the full potential of this simple and successful biotechnology. C1 [Gaxiola, Roberto A.; Regmi, Kamesh] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Hirschi, Kendal D.] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. RP Gaxiola, RA (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ USA. EM Roberto.gaxlola@asu.edu NR 12 TC 2 Z9 2 U1 3 U2 5 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0167-7799 J9 TRENDS BIOTECHNOL JI Trends Biotechnol. PD MAY PY 2016 VL 34 IS 5 BP 347 EP 349 DI 10.1016/j.tibtech.2015.12.016 PG 3 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DL7HD UT WOS:000375810800001 PM 26818803 ER PT J AU Warschefsky, EJ Klein, LL Frank, MH Chitwood, DH Londo, JP von Wettberg, EJB Miller, AJ AF Warschefsky, Emily J. Klein, Laura L. Frank, Margaret H. Chitwood, Daniel H. Londo, Jason P. von Wettberg, Eric J. B. Miller, Allison J. TI Rootstocks: Diversity, Domestication, and Impacts on Shoot Phenotypes SO TRENDS IN PLANT SCIENCE LA English DT Review ID GENE-EXPRESSION PATTERNS; NATURAL ROOT GRAFTS; VITIS-VINIFERA L.; WATER-STRESS; APPLE ROOTSTOCKS; FRUIT-QUALITY; PLANT-GROWTH; PHOSPHORUS ACQUISITION; HORIZONTAL TRANSFER; CROP DOMESTICATION AB Grafting is an ancient agricultural practice that joins the root system (rootstock) of one plant to the shoot (scion) of another. It is most commonly employed in woody perennial crops to indirectly manipulate scion phenotype. While recent research has focused on scions, here we investigate rootstocks, the lesser known half of the perennial crop equation. We review natural grafting, grafting in agriculture, rootstock diversity and domestication, and developing areas of rootstock research, including molecular interactions and rootstock micro-biomes. With growing interest in perennial crops as valuable components of sustainable agriculture, rootstocks provide one mechanism by which to improve and expand woody perennial cultivation in a range of environmental conditions. C1 [Warschefsky, Emily J.; von Wettberg, Eric J. B.] Florida Int Univ, Dept Biol Sci, 11200 Southwest 8th St, Miami, FL 33199 USA. [Warschefsky, Emily J.; von Wettberg, Eric J. B.] Kushlan Trop Sci Inst, Fairchild Trop Bot Garden, 10901 Old Cutler Rd, Coral Gables, FL 33156 USA. [Klein, Laura L.; Miller, Allison J.] St Louis Univ, Dept Biol, 3507 Laclede Ave, St Louis, MO 63103 USA. [Klein, Laura L.; Miller, Allison J.] Missouri Bot Garden, 4344 Shaw Blvd, St Louis, MO 63110 USA. [Frank, Margaret H.; Chitwood, Daniel H.] Donald Danforth Plant Sci Ctr, 975 North Warson Rd, St Louis, MO 63132 USA. [Londo, Jason P.] ARS, USDA, Grape Genet Res Unit, 630 West North St, Geneva, NY 14456 USA. [von Wettberg, Eric J. B.] Florida Int Univ, Int Ctr Trop Bot, 11200 Southwest 8th St, Miami, FL 33199 USA. RP Miller, AJ (reprint author), St Louis Univ, Dept Biol, 3507 Laclede Ave, St Louis, MO 63103 USA.; Miller, AJ (reprint author), Missouri Bot Garden, 4344 Shaw Blvd, St Louis, MO 63110 USA. EM amille75@slu.edu RI Chitwood, Daniel/M-2841-2013 OI Chitwood, Daniel/0000-0003-4875-1447 FU Fairchild Tropical Botanic Garden; Florida International University (FIU) Dissertation Evidence Acquisition Fellowship; NSF DDIG [1501326]; NSF PGRP [1339346, IOS-1523668]; Saint Louis University (SLU) Graduate Research Assistantship; NSF Grape Research Coordination Network Graduate Research Award; Missouri Grape and Wine Institute; SLU Center for Sustainability and Presidential Research Fund FX E.W. is supported by Fairchild Tropical Botanic Garden, a Florida International University (FIU) Dissertation Evidence Acquisition Fellowship, and NSF DDIG 1501326 to E.W. and E.J.B.v.W. E.J.B.v.W. is funded by NSF PGRP 1339346. This work was supported In part by a Saint Louis University (SLU) Graduate Research Assistantship to L.K., a NSF Grape Research Coordination Network Graduate Research Award to L.K., and funding from the Missouri Grape and Wine Institute, SLU Center for Sustainability and Presidential Research Fund to A.J.M. M.F. Is supported by a postdoctoral fellowship from the NSF PGRP (award number IOS-1523668). NR 230 TC 5 Z9 5 U1 31 U2 53 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1360-1385 J9 TRENDS PLANT SCI JI Trends Plant Sci. PD MAY PY 2016 VL 21 IS 5 BP 418 EP 437 DI 10.1016/j.tplants.2015.11.008 PG 20 WC Plant Sciences SC Plant Sciences GA DL7HC UT WOS:000375810600009 PM 26698413 ER PT J AU Holtman, KM Bozzi, DV Franqui-Villanueva, D Offeman, RD Orts, WJ AF Holtman, Kevin M. Bozzi, David V. Franqui-Villanueva, Diana Offeman, Richard D. Orts, William J. TI A pilot-scale steam autoclave system for treating municipal solid waste for recovery of renewable organic content: Operational results and energy usage SO WASTE MANAGEMENT & RESEARCH LA English DT Article DE Municipal solid waste; autoclave; autoclaving; steam classification; renewable organic content; landfill diversion ID FIBER AB A pilot-scale (1800 kg per batch capacity) autoclave used in this study reduces municipal solid waste to a debris contaminated pulp product that is efficiently separated into its renewable organic content and non-renewable organic content fractions using a rotary trommel screen. The renewable organic content can be recovered at nearly 90% efficiency and the trommel rejects are also much easier to sort for recovery. This study provides the evaluation of autoclave operation, including mass and energy balances for the purpose of integration into organic diversion systems. Several methods of cooking municipal solid waste were explored from indirect oil heating only, a combination of oil and direct steam during the same cooking cycle, and steam only. Gross energy requirements averaged 1290 kJ kg(-1) material in vessel, including the weight of free water and steam added during heating. On average, steam recovery can recoup 43% of the water added and 30% of the energy, supplying on average 40% of steam requirements for the next cook. Steam recycle from one vessel to the next can reduce gross energy requirements to an average of 790 kJ kg(-1). C1 [Holtman, Kevin M.; Bozzi, David V.; Franqui-Villanueva, Diana; Offeman, Richard D.; Orts, William J.] USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. RP Holtman, KM (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM kevin.holtman@ars.usda.gov FU USDA [58-3K95-7-1175]; CR3 [58-3K95-7-1175] FX The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This report was funded by a Cooperative Research and Development Agreement [CRADA # 58-3K95-7-1175] between the USDA and CR3. NR 19 TC 2 Z9 2 U1 2 U2 6 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0734-242X EI 1096-3669 J9 WASTE MANAGE RES JI Waste Manage. Res. PD MAY PY 2016 VL 34 IS 5 BP 457 EP 464 DI 10.1177/0734242X16636677 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DL7RE UT WOS:000375837000008 PM 26987737 ER PT J AU Ukaew, S Shi, R Lee, JH Archer, DW Pearlson, M Lewis, KC Bregni, L Shonnard, DR AF Ukaew, Suchada Shi, Rui Lee, Joon Hee Archer, David W. Pearlson, Matthew Lewis, Kristin C. Bregni, Leanne Shonnard, David R. TI Full Chain Life Cycle Assessment of Greenhouse Gases and Energy Demand for Canola-Derived Jet Fuel in North Dakota, United States SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Biofuel sustainability; HEFA; Land use change; Canola rotation; EPIC model; AFTOT model ID DIESEL; OIL AB The success of long-term sustainable biofuel production on agricultural lands is still questionable. To this end, we investigated the effects of crop prices on the changes of agricultural land use for biofuel canola production in three wheat crop management zones in North Dakota. The effects of canola hydroprocessed esters and fatty acids (HEFA) production on greenhouse gas (GHG) emissions and energy demand were investigated along with different allocation methods. The Environmental Policy Integrated Climate (EPIC) and Alternative Fuel Transportation Optimization Tool (AFTOT) models were used to simulate the life cycle assessment (LCA) inputs for two key stages of the HEFA pathway: cultivation and transportation. From the EPIC model results, the increase in canola price had a significant impact on predicted farmer decisions to displace food crops with energy crops and particularly on resulting changes in soil carbon (C). The LCA results suggested that to increase soil C sequestration, energy canola should be grown in the place of the fallow whenever possible to guarantee the long-term soil C sustainability of canola HEFA. Other possible ways to mitigate the GHG emissions included using anhydrous ammonia as the nitrogen fertilizer for cultivation and H-2 integration (use of HEFA coproducts in H-2 production) for HEFA conversion. C1 [Ukaew, Suchada] Naresuan Univ, Dept Ind Engn, Phitsanulok 65000, Thailand. [Shi, Rui; Bregni, Leanne; Shonnard, David R.] Michigan Technol Univ, Dept Chem Engn, Houghton, MI 49931 USA. [Lee, Joon Hee] North Dakota State Water Commiss, Bismarck, ND 58501 USA. [Archer, David W.] ARS, USDA, Mandan, ND 58554 USA. [Pearlson, Matthew; Lewis, Kristin C.] US Dept Transportat, John A Volpe Natl Transportat Syst Ctr, Cambridge, MA 02142 USA. [Shonnard, David R.] Michigan Technol Univ, Sustainable Futures Inst, Houghton, MI 49931 USA. RP Ukaew, S (reprint author), Naresuan Univ, Dept Ind Engn, Phitsanulok 65000, Thailand. EM suchadauk@gmail.com FU Michigan Tech [59-3620-3-002]; USDA-ARS Interagency [60-3620-2-361/USDA-NIFA-BRDI, 2012-10008-19727]; Federal Aviation Administration (FAA) [DTFAVP-14-X-00118, DTFAVP-15-X-00097]; Office of Naval Research [DTFAVP-12-X-00177] FX Funding from the Michigan Tech subaward No. 59-3620-3-002 and USDA-ARS Interagency Agreement No. 60-3620-2-361/USDA-NIFA-BRDI # 2012-10008-19727 is appreciated. Volpe development of AFTOT was funded by the Federal Aviation Administration (FAA) and Office of Naval Research under Interagency Agreement No. DTFAVP-12-X-00177 and by the FAA under Agreement Nos. DTFAVP-14-X-00118 and DTFAVP-15-X-00097. We thank UOP (a Honeywell Company) for HEFA conversion data. NR 36 TC 1 Z9 1 U1 9 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD MAY PY 2016 VL 4 IS 5 BP 2771 EP 2779 DI 10.1021/acssuschemeng.6b00276 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DL3FR UT WOS:000375520100038 ER PT J AU Maltais-Landry, G Scow, K Brennan, E Torbert, E Vitousek, P AF Maltais-Landry, G. Scow, K. Brennan, E. Torbert, E. Vitousek, P. TI Higher flexibility in input N:P ratios results in more balanced phosphorus budgets in two long-term experimental agroecosystems SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Phosphorus use efficiency (PUE); Tomato (Solanum lycopersicum); Corn (Zea mays); Winter wheat (Triticum aestivum); Lettuce (Lactuca sativa); Broccoli (Brassica oleracea); Organic vs. conventional fertilization ID CROP SEEDING RATE; COVER CROP; AGRICULTURAL SOILS; PRODUCTION SYSTEMS; VARIETY AFFECTS; USE EFFICIENCY; MANAGEMENT; NITROGEN; PLANT; FERTILIZATION AB Inefficient phosphorus (P) use in intensive agriculture is common in both organic and conventional systems, resulting in P over-application and soil P build-up. Increasing crop P removal and P recycling within farming systems (e.g., via cover crops) and reducing P inputs lower P surpluses, resulting in more balanced P budgets. Lowering P inputs to reduce soil P surpluses is easier with mineral fertilizers for which nitrogen (N) and P inputs can be decoupled, whereas reducing inputs of organic amendments with a constrained N:P stoichiometry (manures, composts) often results in N under-fertilization and lower yields. We computed farm-gate P budgets for several vegetable and grain cropping systems in two long-term California agricultural experiments that vary in terms of inputs (mineral fertilizers, organic fertilizers, manure, yard compost), cash crops (corn, wheat, tomato, broccoli, lettuce), cover crops (type, frequency) and cropping intensity (biennially, annually or biannually). In organic systems, using manure or compost resulted in high P surpluses, whereas using pelleted or liquid organic fertilizers with higher N:P ratios resulted in smaller P surpluses. Systems receiving mineral fertilizers were often very close to P balance when fertilized regularly. Grain rotations generally had small P deficits whereas vegetable rotations had P surpluses due to lower crop P removal and higher output N:P in vegetables. Phosphorus uptake by cover crops was important (12-25 kg P ha(-1)), but their benefits to soil fertility will depend on the magnitude and timing of P release during residue decomposition. Overall, using organic nutrient sources with a constrained stoichiometry and low N:P ratios resulted in significant P surpluses, confirming the need to use complementary N sources such as N-fixation or N-rich fertilizers to balance P budgets. (C) 2016 Elsevier B.V. All rights reserved. C1 [Maltais-Landry, G.; Vitousek, P.] Dept Biol, Stanford, CA USA. [Scow, K.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Brennan, E.] ARS, USDA, Salinas, CA USA. [Torbert, E.] Univ Calif Davis, Agr Sustainabil Inst, Davis, CA 95616 USA. [Maltais-Landry, G.] Univ British Columbia, Fac Land & Food Syst, 2357 Main Mall, Vancouver, BC V6T 1Z4, Canada. RP Maltais-Landry, G (reprint author), Univ British Columbia, Fac Land & Food Syst, 2357 Main Mall, Vancouver, BC V6T 1Z4, Canada. EM gabriel.maltais-landry@ubc.ca FU Natural Sciences and Engineering Research Council of Canada (NSERC); Fonds Quebecois de la Recherche sur la Nature et les Technologies (FQRNT); Doctoral Dissertation Improvement Grant (DDIG) from the National Science Foundation (NSF) FX We thank I. Herrera and the Agricultural Sustainability Institute at UC-Davis for access to archived samples and data at Russell Ranch, and two anonymous reviewers and the editor for meaningful comments that improved this manuscript substantially. This research was partially funded by a graduate fellowship to G. Maltais-Landry from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Fonds Quebecois de la Recherche sur la Nature et les Technologies (FQRNT), and a Doctoral Dissertation Improvement Grant (DDIG) from the National Science Foundation (NSF) to P. Vitousek and G. Maltais-Landry. NR 39 TC 1 Z9 1 U1 20 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD MAY 1 PY 2016 VL 223 BP 197 EP 210 DI 10.1016/j.agee.2016.03.007 PG 14 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DL0QK UT WOS:000375337400022 ER PT J AU Brito, A Fedosov, SN Miller, JW Green, R Uauy, R Allen, LH AF Brito, Alex Fedosov, Sergey N. Miller, Joshua W. Green, Ralph Uauy, Ricardo Allen, Lindsay H. TI Vitamin B-12 and neuropathy in the elderly Reply SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Letter ID COMBINED INDICATOR C1 [Brito, Alex; Allen, Lindsay H.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Fedosov, Sergey N.] Aarhus Univ, Dept Mol Biol & Genet, Aarhus, Denmark. [Miller, Joshua W.] Rutgers State Univ, Dept Nutr Sci, New Brunswick, NJ 08903 USA. [Miller, Joshua W.; Green, Ralph] Univ Calif Davis, Dept Pathol & Lab Med, Davis, CA 95616 USA. [Uauy, Ricardo] Univ Chile, Inst Nutr & Food Technol, Santiago, Chile. RP Allen, LH (reprint author), USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. EM lindsay.allen@ars.usda.gov NR 4 TC 0 Z9 0 U1 1 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2016 VL 103 IS 5 BP 1379 EP 1379 DI 10.3945/ajcn.116.133322 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DL0VM UT WOS:000375350700023 PM 27140535 ER PT J AU Kieffer, DA Piccolo, BD Vaziri, ND Liu, SM Lau, WL Khazaeli, M Nazertehrani, S Moore, ME Marco, ML Martin, RJ Adams, SH AF Kieffer, Dorothy A. Piccolo, Brian D. Vaziri, Nosratola D. Liu, Shuman Lau, Wei L. Khazaeli, Mahyar Nazertehrani, Sohrab Moore, Mary E. Marco, Maria L. Martin, Roy J. Adams, Sean H. TI Resistant starch alters gut microbiome and metabolomic profiles concurrent with amelioration of chronic kidney disease in rats SO AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY LA English DT Article DE dietary fiber; resistant starch; chronic kidney disease; uremic retention solutes; gut microbiota ID CHRONIC-RENAL-FAILURE; HUMAN LARGE-INTESTINE; HIGH AMYLOSE CORNSTARCH; CHAIN FATTY-ACIDS; P-CRESYL SULFATE; DIETARY FIBER; CARBOHYDRATE AVAILABILITY; GASTROINTESTINAL-TRACT; HEMODIALYSIS-PATIENTS; ULCERATIVE-COLITIS AB Patients and animals with chronic kidney disease (CKD) exhibit profound alterations in the gut environment including shifts in microbial composition, increased fecal pH, and increased blood levels of gut microbe-derived metabolites (xenometabolites). The fermentable dietary fiber high amylose maize-resistant starch type 2 (HAMRS2) has been shown to alter the gut milieu and in CKD rat models leads to markedly improved kidney function. The aim of the present study was to identify specific cecal bacteria and cecal, blood, and urinary metabolites that associate with changes in kidney function to identify potential mechanisms involved with CKD amelioration in response to dietary resistant starch. Male Sprague-Dawley rats with adenine-induced CKD were fed a semipurified low-fiber diet or a high-fiber diet [59% (wt/wt) HAMRS2] for 3 wk (n = 9 rats/group). The cecal microbiome was characterized, and cecal contents, serum, and urine metabolites were analyzed. HAMRS2-fed rats displayed decreased cecal pH, decreased microbial diversity, and an increased Bacteroidetes-to-Firmicutes ratio. Several uremic retention solutes were altered in the cecal contents, serum, and urine, many of which had strong correlations with specific gut bacteria abundances, i.e., serum and urine indoxyl sulfate were reduced by 36% and 66%, respectively, in HAMRS2-fed rats and urine p-cresol was reduced by 47% in HAMRS2-fed rats. Outcomes from this study were coincident with improvements in kidney function indexes and amelioration of CKD outcomes previously reported for these rats, suggesting an important role for microbial-derived factors and gut microbe metabolism in regulating host kidney function. C1 [Kieffer, Dorothy A.; Martin, Roy J.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA USA. [Kieffer, Dorothy A.; Adams, Sean H.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Kieffer, Dorothy A.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Vaziri, Nosratola D.; Liu, Shuman; Lau, Wei L.; Khazaeli, Mahyar; Nazertehrani, Sohrab] Univ Calif Irvine, Div Nephrol, Irvine, CA USA. [Moore, Mary E.; Marco, Maria L.] Univ Calif Irvine, Dept Food Sci & Technol, Irvine, CA USA. [Piccolo, Brian D.; Adams, Sean H.] Univ Arkansas Med Sci, Arkansas Childrens Nutr Ctr, Little Rock, AR 72205 USA. [Piccolo, Brian D.; Adams, Sean H.] Univ Arkansas Med Sci, Dept Pediat, Little Rock, AR 72205 USA. RP Adams, SH (reprint author), Arkansas Childrens Nutr Ctr, 15 Childrens Way, Little Rock, AR 72202 USA. EM shadams@uams.edu FU T32 training award - National Center for Advancing Translational Sciences, National Institutes of Health [UL1 TR000002, TL1 TR000133]; Danish Council for Strategic Research Project [10-093526]; USDA-ARS [2032-51530-022-00D, 6026-51000-010-05S]; Arkansas Biosciences Institute, the major research component of the Arkansas Tobacco Settlement Proceeds Act FX The project was supported in part by a T32 training award (to D. A. Kieffer) funded by the National Center for Advancing Translational Sciences, National Institutes of Health, through grant number UL1 TR000002 and linked award TL1 TR000133. Additional funding was provided by the Danish Council for Strategic Research Project 10-093526, USDA-ARS Projects 2032-51530-022-00D and 6026-51000-010-05S, and in part by the Arkansas Biosciences Institute, the major research component of the Arkansas Tobacco Settlement Proceeds Act of 2000. The USDA is an equal opportunity provider and employer. NR 86 TC 10 Z9 10 U1 9 U2 21 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 1931-857X EI 1522-1466 J9 AM J PHYSIOL-RENAL JI Am. J. Physiol.-Renal Physiol. PD MAY 1 PY 2016 VL 310 IS 9 BP F857 EP F871 DI 10.1152/ajprenal.00513.2015 PG 15 WC Physiology; Urology & Nephrology SC Physiology; Urology & Nephrology GA DK7ON UT WOS:000375115700007 PM 26841824 ER PT J AU Geary, TW Kelly, WL Spickard, DS Larson, CK Grings, EE Ansotegui, RP AF Geary, T. W. Kelly, W. L. Spickard, D. S. Larson, C. K. Grings, E. E. Ansotegui, R. P. TI Effect of supplemental trace mineral level and form on peripubertal bulls SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Bull; Puberty; Trace minerals ID ZINC-DEFICIENCY; GONADOTROPIN-SECRETION; TESTICULAR DEVELOPMENT; EMISSION-SPECTROSCOPY; REPRODUCTIVE CAPACITY; ELEMENT DEFICIENCIES; LUTEINIZING-HORMONE; POSTPUBERAL CHANGES; DIFFERENT BREEDS; HOLSTEIN BULLS AB Objectives were to determine if supplemental trace mineral levels and/or forms (sulfate and metal amino acid complexes) influence age at puberty, semen quality, endocrine status, and scrotal circumference in peripubertal bulls. Fifty peripubertal bulls were blocked by age and scrotal circumference and assigned to one of five treatments: (1) lx sulfate form (1S); (2) 1x complexed form (1C); (3) 1S + 1C (2SC); (4) 1S+2 x 1C (3SCC); and (5) 3 x 1S (3S). Each 1x supplementation level contained 360 mg Zn, 125 mg Cu, 200 mg Mn and 12.5 mg Co. Liver biopsies were collected on d - 21 and 100, and scrotal circumference, semen, and blood samples were collected on d - 14, 14, 42, 70, and 98. All bulls were deficient in Cu yet adequate in Zn on d - 21. Following 100 d on treatment, liver Zn concentrations decreased (P < 0.01) and liver Cu concentrations increased (P < 0.01) in bulls regardless of treatment. Day 100 liver Zn concentrations were similar (P = 0.50) across treatments, but liver Cu concentrations were greater (P = 0.07) in 3SCC and 3S bulls compared to 1C and 1S bulls, whereas 2SC bulls were intermediate. Bulls fed complexed minerals tended to reach puberty after fewer (P = 0.11) days on treatment (43.9 +/- 5.7 d) than bulls fed only sulfate minerals (58.5 +/- 6.7 d). Supplementing complexed Cu and Zn to prepubertal bulls may lower the age at puberty, however, no differences (P >= 0.40) in semen characteristics or scrotal measurements (P >= 0.11) were observed. (C) 2016 Published by Elsevier B.V. C1 [Geary, T. W.; Kelly, W. L.; Grings, E. E.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Spickard, D. S.] World West Sire Serv, 25 Winterpast Lane, Joliet, MT 59041 USA. [Larson, C. K.] Zinpro Corp, 10400 Viking Dr,Suite 240, Eden Prairie, MN 55344 USA. [Ansotegui, R. P.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USA. [Kelly, W. L.] 16 Mastin Rd, Kinsey, MT 59338 USA. [Grings, E. E.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. [Ansotegui, R. P.] 124 Paradise Dr, Livingston, MT 59047 USA. RP Geary, TW (reprint author), 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM tom.geary@ars.usda.gov NR 47 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 EI 1873-2232 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD MAY PY 2016 VL 168 BP 1 EP 9 DI 10.1016/j.anireprosci.2016.02.018 PG 9 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA DK4TW UT WOS:000374914200001 PM 26968246 ER PT J AU Higgins, SA Welsh, A Orellana, LH Konstantinidis, KT Chee-Sanford, JC Sanford, RA Schadt, CW Loffler, FE AF Higgins, Steven A. Welsh, Allana Orellana, Luis H. Konstantinidis, Konstantinos T. Chee-Sanford, Joanne C. Sanford, Robert A. Schadt, Christopher W. Loffler, Frank E. TI Detection and Diversity of Fungal Nitric Oxide Reductase Genes (p450nor) in Agricultural Soils SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID MULTIPLE SEQUENCE ALIGNMENT; N2O PRODUCTION; FUSARIUM-OXYSPORUM; CYTOCHROME P450NOR; PROTEIN EVOLUTION; DENITRIFICATION; BACTERIAL; EMISSION; DATABASE; NITRATE AB Members of the Fungi convert nitrate (NO3-) and nitrite (NO2-) to gaseous nitrous oxide (N2O) (denitrification), but the fungal contributions to N loss from soil remain uncertain. Cultivation-based methodologies that include antibiotics to selectively assess fungal activities have limitations, and complementary molecular approaches to assign denitrification potential to fungi are desirable. Microcosms established with soils from two representative U.S. Midwest agricultural regions produced N2O from added NO3- or NO2- in the presence of antibiotics to inhibit bacteria. Cultivation efforts yielded 214 fungal isolates belonging to at least 15 distinct morphological groups, 151 of which produced N2O from NO2-. Novel PCR primers targeting the p450nor gene, which encodes the nitric oxide (NO) reductase responsible for N2O production in fungi, yielded 26 novel p450nor amplicons from DNA of 37 isolates and 23 amplicons from environmental DNA obtained from two agricultural soils. The sequences shared 54 to 98% amino acid identity with reference P450nor sequences within the phylum Ascomycota and expand the known fungal P450nor sequence diversity. p450nor was detected in all fungal isolates that produced N2O from NO2-, whereas nirK (encoding the NO-forming NO2- reductase) was amplified in only 13 to 74% of the N2O-forming isolates using two separate nirK primer sets. Collectively, our findings demonstrate the value of p450nor-targeted PCR to complement existing approaches to assess the fungal contributions to denitrification and N2O formation. IMPORTANCE A comprehensive understanding of the microbiota controlling soil N loss and greenhouse gas (N2O) emissions is crucial for sustainable agricultural practices and addressing climate change concerns. We report the design and application of a novel PCR primer set targeting fungal p450nor, a biomarker for fungal N2O production, and demonstrate the utility of the new approach to assess fungal denitrification potential in fungal isolates and agricultural soils. These new PCR primers may find application in a variety of biomes to assess the fungal contributions to N loss and N2O emissions. C1 [Higgins, Steven A.; Schadt, Christopher W.; Loffler, Frank E.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. [Loffler, Frank E.] Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA. [Loffler, Frank E.] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA. [Schadt, Christopher W.; Loffler, Frank E.] Univ Tennessee & Oak Ridge Natl Lab UT ORNL Joint, Oak Ridge, TN USA. [Schadt, Christopher W.; Loffler, Frank E.] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA. [Welsh, Allana; Sanford, Robert A.] Univ Illinois, Dept Geol, Urbana, IL 61801 USA. [Chee-Sanford, Joanne C.] ARS, USDA, Urbana, IL USA. [Orellana, Luis H.; Konstantinidis, Konstantinos T.] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA. [Konstantinidis, Konstantinos T.] Georgia Inst Technol, Sch Biol, Atlanta, GA 30332 USA. RP Loffler, FE (reprint author), Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA.; Loffler, FE (reprint author), Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA.; Loffler, FE (reprint author), Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA.; Loffler, FE (reprint author), Univ Tennessee & Oak Ridge Natl Lab UT ORNL Joint, Oak Ridge, TN USA.; Loffler, FE (reprint author), Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA. EM frank.loeffler@utk.edu RI Schadt, Christopher/B-7143-2008; OI Schadt, Christopher/0000-0001-8759-2448; Higgins, Steven/0000-0002-5209-5000 FU U.S. Department of Energy (DOE), Office of Biological and Environmental Research, Genomic Science Program [DE-SC0006662] FX This research was supported by the U.S. Department of Energy (DOE), Office of Biological and Environmental Research, Genomic Science Program (award DE-SC0006662). NR 70 TC 0 Z9 0 U1 8 U2 23 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2016 VL 82 IS 10 BP 2919 EP 2928 DI 10.1128/AEM.00243-16 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DK9EU UT WOS:000375234400005 PM 26969694 ER PT J AU Reardon, CL Wuest, SB AF Reardon, Catherine L. Wuest, Stewart B. TI Soil amendments yield persisting effects on the microbial communities-a 7-year study SO APPLIED SOIL ECOLOGY LA English DT Article DE Soil amendment; Soil enzyme activity; Fungi; Bacteria; Community ID BETA-GLUCOSAMINIDASE ACTIVITY; ARYLAMIDASE ACTIVITY; ENZYME-ACTIVITIES; AGRICULTURAL SOIL; SUGARCANE SUCROSE; CROPPING SYSTEMS; WHEAT-FALLOW; ARABLE SOIL; CARBON; BIOSOLIDS AB Soil microbial communities are sensitive to carbon amendments and largely control the decomposition and accumulation of soil organic matter. In this study, we evaluated whether the type of carbon amendment applied to wheat-cropped or fallow soil imparted lasting effects on the microbial community with detectable differences in activity, population size, or community structure after a period of seven years post-amendment. The microbial communities from the top 10 cm of soil were analyzed for activity related to C-cycling (glucosidase, galactosidase), P-cycling (acid phosphatase), S-cycling (arylsulfatase), and N-cycling (beta-glucosaminidase, arylamidase), in addition to fungal and bacterial abundance and structure. The amendments were applied at similar carbon rates for five years under annual wheat or continuous fallow and included cotton linters, sucrose, wheat residue, composted wheat residue, brassica residue, wood sawdust, alfalfa feed pellets, manure, biosolid and a no treatment control. Two crops, brassica and grass, were in the fallow treatments. The majority of the communities in the amended soils were not distinguishable from the no-treatment control. For amendments and crops that produced changes, significant differences in the population size and community structure were observable for fungi but not bacteria. Wood, sugar, and grass cropping produced the most pronounced effects on enzyme activity, fungal abundance and structure. Overall, the species of the planted crop had a significant effect on the soil enzyme activity and population size of fungi, with the greatest values under grass compared to wheat or brassica. The microbial communities were differentially affected by C source amendments in which the persistency of change and the aspect of the community affected (i.e. function, size, structure, kingdom) were dependent on amendment type. Published by Elsevier B.V. C1 [Reardon, Catherine L.; Wuest, Stewart B.] ARS, USDA, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. RP Reardon, CL (reprint author), ARS, USDA, Columbia Plateau Conservat Res Ctr, 48037 Tubbs Ranch Rd, Adams, OR 97810 USA. EM Catherine.Reardon@ars.usda.gov FU USDA-ARS [216, 212] FX Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This research was conducted under USDA-ARS national programs Agricultural System Competitiveness and Sustainability (NP# 216) and Climate Change, Soils, and Emissions (NP# 212). NR 61 TC 3 Z9 3 U1 13 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD MAY PY 2016 VL 101 BP 107 EP 116 DI 10.1016/j.apsoil.2015.12.013 PG 10 WC Soil Science SC Agriculture GA DK7QR UT WOS:000375121300014 ER PT J AU Esteve-Gassent, MD Castro-Arellano, I Feria-Arroyo, TP Patino, R Li, AY Medina, RF de Leon, AAP AF Esteve-Gassent, Maria D. Castro-Arellano, Ivan Feria-Arroyo, Teresa P. Patino, Ramiro Li, Andrew Y. Medina, Raul F. de Leon, Adalberto A. Perez TI TRANSLATING ECOLOGY, PHYSIOLOGY, BIOCHEMISTRY, AND POPULATION GENETICS RESEARCH TO MEET THE CHALLENGE OF TICK AND TICK-BORNE DISEASES IN NORTH AMERICA SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE tick-borne diseases; climate change; tick physiology; questing behavior; genetic diversity ID RHIPICEPHALUS BOOPHILUS MICROPLUS; VECTOR IXODES-SCAPULARIS; BURGDORFERI SENSU-LATO; WHITE-TAILED DEER; EASTERN UNITED-STATES; SOUTHERN CATTLE TICK; CLIMATE-BASED MODEL; LYME-DISEASE; ACARI IXODIDAE; BORRELIA-BURGDORFERI AB Emerging and re-emerging tick-borne diseases threaten public health and the wellbeing of domestic animals and wildlife globally. The adoption of an evolutionary ecology framework aimed to diminish the impact of tick-borne diseases needs to be part of strategies to protect human and animal populations. We present a review of current knowledge on the adaptation of ticks to their environment, and the impact that global change could have on their geographic distribution in North America. Environmental pressures will affect tick population genetics by selecting genotypes able to withstand new and changing environments and by altering the connectivity and isolation of several tick populations. Research in these areas is particularly lacking in the southern United States and most of Mexico with knowledge gaps on the ecology of these diseases, including a void in the identity of reservoir hosts for several tick-borne pathogens. Additionally, the way in which anthropogenic changes to landscapes may influence tick-borne disease ecology remains to be fully understood. Enhanced knowledge in these areas is needed in order to implement effective and sustainable integrated tick management strategies. We propose to refocus ecology studies with emphasis on metacommunity-based approaches to enable a holistic perspective addressing whole pathogen and host assemblages. Network analyses could be used to develop mechanistic models involving multihost-pathogen communities. An increase in our understanding of the ecology of tick-borne diseases across their geographic distribution will aid in the design of effective area-wide tick control strategies aimed to diminish the burden of pathogens transmitted by ticks. (C) 2016 Wiley Periodicals, Inc. C1 [Esteve-Gassent, Maria D.] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. [Castro-Arellano, Ivan] Texas State Univ, Coll Sci & Engn, Dept Biol, San Marcos, TX USA. [Feria-Arroyo, Teresa P.; Patino, Ramiro] Univ Texas Rio Grande Valley, Dept Biol, Edinburg, TX USA. [Li, Andrew Y.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD USA. [Medina, Raul F.] Texas A&M Univ, Coll Agr & Life Sci, Dept Entomol, College Stn, TX 77843 USA. [de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX USA. [de Leon, Adalberto A. Perez] USDA ARS, Vet Pest Genom Ctr, Kerrville, TX USA. RP Esteve-Gassent, MD (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. EM MEsteve-Gassent@cvm.tamu.edu FU Texas AgriLife TEX6579 [I9524]; Research Enhancement Program (Texas State University); National Institute of Health; NIAID [1R21AI107380-01A1]; NIH [5R25GM100866-02] FX Grant sponsor: Texas AgriLife TEX6579; Grant number: I9524; Grant sponsor: Research Enhancement Program (Texas State University); Grant sponsor: National Institute of Health; Grant sponsor: NIAID; Grant number: 1R21AI107380-01A1; Grant sponsor: NIH; Grant number: 5R25GM100866-02. NR 201 TC 3 Z9 3 U1 22 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD MAY PY 2016 VL 92 IS 1 BP 38 EP 64 DI 10.1002/arch.21327 PG 27 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA DK2UQ UT WOS:000374770300004 PM 27062414 ER PT J AU Danziger, J Young, RL Shea, MK Tracy, RP Ix, JH Jenny, NS Mukamal, KJ AF Danziger, John Young, Rebekah L. Shea, M. Kyla Tracy, Russell P. Ix, Joachim H. Jenny, Nancy S. Mukamal, Kenneth J. TI Vitamin K-Dependent Protein Activity and Incident Ischemic Cardiovascular Disease: The Multi-Ethnic Study of Atherosclerosis SO ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY LA English DT Article DE cardiovascular diseases; prothrombin; risk factors; vitamin K ID MATRIX GLA-PROTEIN; VASCULAR SMOOTH-MUSCLE; ARREST-SPECIFIC GENE; GROWTH ARREST; HEMODIALYSIS-PATIENTS; ARTERY CALCIFICATION; ENDOTHELIAL-CELLS; MER RECEPTOR; GAS6; PERIOSTIN AB Objective-Vitamin K-dependent proteins (VKDPs), which require post-translational modification to achieve biological activity, seem to contribute to thrombus formation, vascular calcification, and vessel stiffness. Whether VKDP activity is prospectively associated with incident cardiovascular disease has not been studied. Approach and Results-VKDP activity was determined by measuring circulating des-gamma-carboxy prothrombin concentrations in a random sample of 709 multiethnic adults free of cardiovascular disease drawn from the Multi-Ethnic Study of Atherosclerosis (MESA). Lower des-gamma-carboxy prothrombin concentrations reflect greater VKDP activity. Subjects were followed up for the risk of ischemic cardiovascular disease (coronary heart disease, stroke, and fatal cardiovascular disease) for 11.0 years of follow-up. A total of 75 first ischemic CVD events occurred during follow-up. The incidence of ischemic cardiovascular disease increased progressively across des-gamma-carboxy prothrombin quartiles, with event rates of 5.9 and 11.7 per 1000 person-years in the lowest and highest quartiles. In analyses adjusted for traditional cardiovascular risk factors and measures of vitamin K intake, a doubling of des-gamma-carboxy prothrombin concentration was associated with a 1.53 (95% confidence interval, 1.09-2.13; P=0.008) higher risk of incident ischemic cardiovascular disease. The association was consistent across strata of participants with diabetes mellitus, hypertension, renal impairment, and low vitamin K nutritional intake. Conclusions-In this sample of middle-aged and older adults, VKDP activity was associated with incident ischemic cardiovascular events. Further studies to understand the role of this large class of proteins in cardiovascular disease are warranted. C1 [Danziger, John; Mukamal, Kenneth J.] Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USA. [Young, Rebekah L.] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Shea, M. Kyla] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tracy, Russell P.] Univ Vermont, Coll Med, Burlington, VT 05405 USA. [Jenny, Nancy S.] Univ Vermont, Dept Pathol, Burlington, VT 05405 USA. [Ix, Joachim H.] Vet Affairs San Diego Healthcare Syst, Nephrol Sect, San Diego, CA USA. RP Danziger, J (reprint author), Beth Israel Deaconess Med Ctr, Div Nephrol, 185 Pilgrim Rd, Boston, MA 02215 USA. EM jdanzige@bidmc.harvard.edu FU National Heart, Lung, and Blood Institute [N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, N01-HC-95169]; National Center for Research Resources [UL1-TR-000040, UL1-TR-001079] FX This research was supported by a Normon S. Coplon grant from Satellite Health Care (J. Danziger), contracts N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, and N01-HC-95169 from the National Heart, Lung, and Blood Institute, and by grants UL1-TR-000040 and UL1-TR-001079 from National Center for Research Resources. The authors thank the other investigators, the staff, and the participants of the Multi-Ethnic Study of Atherosclerosis (MESA) study for their valuable contributions. NR 45 TC 2 Z9 2 U1 1 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1079-5642 EI 1524-4636 J9 ARTERIOSCL THROM VAS JI Arterioscler. Thromb. Vasc. Biol. PD MAY PY 2016 VL 36 IS 5 BP 1037 EP 1042 DI 10.1161/ATVBAHA.116.307273 PG 6 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA DK9DF UT WOS:000375230000035 PM 27034472 ER PT J AU Faust, DR Moore, MT Emison, GA Rush, SA AF Faust, Derek R. Moore, Matthew T. Emison, Gerald Andrews Rush, Scott A. TI Potential Implications of Approaches to Climate Change on the Clean Water Rule Definition of "Waters of the United States" SO BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article DE Clean Water Act; Climate change; Policy; Waters of the United States; Connectivity ID MANAGEMENT; RESOURCES; TEMPERATURE; SENSITIVITY; ECOSYSTEMS; CALIFORNIA; TOXICOLOGY; STRESSORS; POLLUTION; IMPACTS AB The 1972 Clean Water Act was passed to protect chemical, physical, and biological integrity of United States' waters. The U.S. Environmental Protection Agency and U.S. Army Corps of Engineers codified a new "waters of the United States" rule on June 29, 2015, because several Supreme Court case decisions caused confusion with the existing rule. Climate change could affect this rule through connectivity between groundwater and surface waters; floodplain waters and the 100-year floodplain; changes in jurisdictional status; and sea level rise on coastal ecosystems. Four approaches are discussed for handling these implications: (1) "Wait and see"; (2) changes to the rule; (3) use guidance documents; (4) Congress statutorily defining "waters of the United States." The approach chosen should be legally defensible and achieved in a timely fashion to provide protection to "waters of the United States" in proactive consideration of scientifically documented effects of climate change on aquatic ecosystems. C1 [Faust, Derek R.; Rush, Scott A.] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Box 9690, Mississippi State, MS 39762 USA. [Moore, Matthew T.] USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, 598 McElroy Dr, Oxford, MS 38655 USA. [Emison, Gerald Andrews] Mississippi State Univ, Dept Polit Sci & Publ Adm, Box PC, Mississippi State, MS 39762 USA. RP Moore, MT (reprint author), USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, 598 McElroy Dr, Oxford, MS 38655 USA. EM drf107@msstate.edu; Matt.Moore@ARS.USDA.GOV NR 37 TC 1 Z9 1 U1 10 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0007-4861 EI 1432-0800 J9 B ENVIRON CONTAM TOX JI Bull. Environ. Contam. Toxicol. PD MAY PY 2016 VL 96 IS 5 BP 565 EP 572 DI 10.1007/s00128-016-1773-z PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DJ7NE UT WOS:000374397300002 PM 26979963 ER PT J AU Railsback, SF Harvey, BC Kupferberg, SJ Lang, MM McBain, S Welsh, HH AF Railsback, Steven F. Harvey, Bret C. Kupferberg, Sarah J. Lang, Margaret M. McBain, Scott Welsh, Hart H., Jr. TI Modeling potential river management conflicts between frogs and salmonids SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID RANA-BOYLII; CALIFORNIA RIVER; TRINITY RIVER; FLOW; METAMORPHOSIS; POPULATIONS; PATTERNS; HABITAT; LARVAL; GROWTH AB Management of regulated rivers for yellow-legged frogs (Rana boylii) and salmonids exemplifies potential conflicts among species adapted to different parts of the natural flow and temperature regimes. Yellow-legged frogs oviposit in rivers in spring and depend on declining flows and warming temperatures for egg and tadpole survival and growth, whereas salmonid management can include high spring flows and low-temperature reservoir releases. We built a model of how flow and temperature affect frog breeding success. Its mechanisms include adults selecting oviposition sites to balance risks of egg dewatering by decreasing flow versus scouring by high flow, temperature effects on development, habitat selection by tadpoles, and mortality via dewatering and scouring. In simulations of a regulated river managed primarily for salmonids, below-natural temperatures delayed tadpole metamorphosis into froglets, which can reduce overwinter survival. However, mitigating this impact via higher temperatures was predicted to cause adults to oviposit before spring flow releases for salmonids, which then scoured the egg masses. The relative timing of frog oviposition and high flow releases appears critical in determining conflicts between salmonid and frog management. C1 [Railsback, Steven F.; Lang, Margaret M.] Lang Railsback & Associates, 250 Calif Ave, Arcata, CA 95521 USA. [Harvey, Bret C.; Welsh, Hart H., Jr.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1700 Bayview Dr, Arcata, CA 95521 USA. [Kupferberg, Sarah J.] Univ Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA. [Lang, Margaret M.] Humboldt State Univ, Dept Environm Resources Engn, Arcata, CA 95521 USA. [McBain, Scott] McBain Associates, Arcata, CA 95521 USA. RP Railsback, SF (reprint author), Lang Railsback & Associates, 250 Calif Ave, Arcata, CA 95521 USA. EM Steve@LangRailsback.com FU US Forest Service, Pacific Southwest Research Station (PSW) Internal Competitive Grants Program FX Funding for this project was provided by US Forest Service, Pacific Southwest Research Station (PSW) Internal Competitive Grants Program. The topographic and bathymetric survey of the study site was conducted by GMA Hydrology Inc., Arcata, California. Additional data collection and analysis support was provided by Diane Southerland Montoya, Rodney Nakamoto, Clara Wheeler, and Jason White of PSW. NR 41 TC 2 Z9 2 U1 71 U2 72 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD MAY PY 2016 VL 73 IS 5 BP 773 EP 784 DI 10.1139/cjfas-2015-0267 PG 12 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DL1XG UT WOS:000375425900005 ER PT J AU Li, LF Li, WH Ballard, T Sun, G Jeuland, M AF Li, Laifang Li, Wenhong Ballard, Tristan Sun, Ge Jeuland, Marc TI CMIP5 model simulations of Ethiopian Kiremt-season precipitation: current climate and future changes SO CLIMATE DYNAMICS LA English DT Article DE Ethiopian Kiremt-season precipitation; CMIP5 model simulations; Climate change impact; North Atlantic subtropical high ID SCALE ATMOSPHERIC CIRCULATION; SUMMER RAINFALL VARIABILITY; SOUTHEASTERN UNITED-STATES; JUNE-SEPTEMBER RAINFALL; SURFACE TEMPERATURE; AFRICAN RAINFALL; NORTH-ATLANTIC; INDIAN-OCEAN; WEST-AFRICA; INTENSIFICATION AB Kiremt-season (June-September) precipitation provides a significant water supply for Ethiopia, particularly in the central and northern regions. The response of Kiremt-season precipitation to climate change is thus of great concern to water resource managers. However, the complex processes that control Kiremt-season precipitation challenge the capability of general circulation models (GCMs) to accurately simulate precipitation amount and variability. This in turn raises questions about their utility for predicting future changes. This study assesses the impact of climate change on Kiremt-season precipitation using state-of-the-art GCMs participating in the Coupled Model Intercomparison Project Phase 5. Compared to models with a coarse resolution, high-resolution models (horizontal resolution < 2A degrees) can more accurately simulate precipitation, most likely due to their ability to capture precipitation induced by topography. Under the Representative Concentration Pathway (RCP) 4.5 scenario, these high-resolution models project an increase in precipitation over central Highlands and northern Great Rift Valley in Ethiopia, but a decrease in precipitation over the southern part of the country. Such a dipole pattern is attributable to the intensification of the North Atlantic subtropical high (NASH) in a warmer climate, which influences Ethiopian Kiremt-season precipitation mainly by modulating atmospheric vertical motion. Diagnosis of the omega equation demonstrates that an intensified NASH increases (decreases) the advection of warm air and positive vorticity into the central Highlands and northern Great Rift Valley (southern part of the country), enhancing upward motion over the northern Rift Valley but decreasing elsewhere. Under the RCP 4.5 scenario, the high-resolution models project an intensification of the NASH by 15 (3 x 10(5) m(2) s(-2)) geopotential meters (stream function) at the 850-hPa level, contributing to the projected precipitation change over Ethiopia. The influence of the NASH on Kiremt-season precipitation becomes more evident in the future due to the offsetting effects of two other major circulation systems: the East African Low-level Jet (EALLJ) and the Tropical Easterly Jet (TEJ). The high-resolution models project a strengthening of the EALLJ, but a weakening of the TEJ. Future changes in the EALLJ and TEJ will drive this precipitation system in opposite directions, leading to small or no net changes in precipitation in Ethiopia. C1 [Li, Laifang; Li, Wenhong] Duke Univ, Nicholas Sch Environm, Earth & Ocean Sci, 321C Old Chem Bldg,POB 90227, Durham, NC 27708 USA. [Ballard, Tristan] Duke Univ, Trinity Coll Arts & Sci, Environm Sci, Durham, NC 27708 USA. [Sun, Ge] USDA Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC 27606 USA. [Jeuland, Marc] Duke Univ, Sanford Sch Publ Policy, Durham, NC 27708 USA. [Jeuland, Marc] Duke Univ, Duke Global Hlth Inst, Durham, NC 27708 USA. [Li, Laifang] Woods Hole Oceanog Inst, Dept Phys Oceanog, Woods Hole, MA 02543 USA. RP Li, WH (reprint author), Duke Univ, Nicholas Sch Environm, Earth & Ocean Sci, 321C Old Chem Bldg,POB 90227, Durham, NC 27708 USA. EM wenhong.li@duke.edu FU NSF Grant [AGS-1147608, NIH-1R21AG044294-01A1]; Postdoctoral Scholar Program at the Woods Hole Oceanographic Institution; Ocean and Climate Change Institute FX We acknowledge the World Climate Research Programme's Working Group on Coupled Modeling, which is responsible for CMIP, and we thank the climate modeling groups (listed in Table 1 of this paper) for producing and making available their model output. For CMIP, the U.S. Department of Energy's Program for Climate Model Diagnosis and Intercomparison provides coordinating support and led development of software infrastructure in partnership with the Global Organization for Earth System Science Portals. Part of the CMIP5 model output analyzed in this study is provided by the WHOI CMIP5 Community Storage Server, Woods Hole Oceanographic Institution, Woods Hole, MA, USA from their website at http://cmip5.whoi.edu/. The authors thank Dr. Brant Liebmann and another two anonymous reviewers for insightful comments. This work is supported by the NSF Grant AGS-1147608 and NIH-1R21AG044294-01A1. The group also benefited from pilot support from the Provost's Office at Duke University and an interdisciplinary collaboration of social and natural scientists working on water and climate change issues at Duke. L. Li is partially supported by the Postdoctoral Scholar Program at the Woods Hole Oceanographic Institution, with funding provided by the Ocean and Climate Change Institute. NR 44 TC 0 Z9 0 U1 7 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0930-7575 EI 1432-0894 J9 CLIM DYNAM JI Clim. Dyn. PD MAY PY 2016 VL 46 IS 9-10 BP 2883 EP 2895 DI 10.1007/s00382-015-2737-4 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DK5OU UT WOS:000374970200012 ER PT J AU Pachepsky, Y Shelton, D Dorner, S Whelan, G AF Pachepsky, Yakov Shelton, Daniel Dorner, Sarah Whelan, Gene TI Can E-coli or thermotolerant coliform concentrations predict pathogen presence or prevalence in irrigation waters? SO CRITICAL REVIEWS IN MICROBIOLOGY LA English DT Review DE Campylobacter; Cryptosporidium; food-borne illnesses; produce; Salmonella ID FECAL-INDICATOR CONCENTRATIONS; FRESH-WATER; UNITED-STATES; MICROBIAL INDICATORS; SURFACE WATERS; CAMPYLOBACTER-JEJUNI; LAND-USE; CRYPTOSPORIDIUM; GIARDIA; SALMONELLA AB An increase in food-borne illnesses in the United States has been associated with fresh produce consumption. Irrigation water presents recognized risks for microbial contamination of produce. Water quality criteria rely on indicator bacteria. The objective of this review was to collate and summarize experimental data on the relationships between pathogens and thermotolerant coliform (THT) and/or generic E. coli, specifically focusing on surface fresh waters used in or potentially suitable for irrigation agriculture. We analyzed peer-reviewed publications in which concentrations of E. coli or THT coliforms in surface fresh waters were measured along with concentrations of one or more of waterborne and food-borne pathogenic organisms. The proposed relationships were significant in 35% of all instances and not significant in 65% of instances. Coliform indicators alone cannot provide conclusive, non-site-specific and non-pathogen-specific information about the presence and/or concentrations of most important pathogens in surface waters suitable for irrigation. Standards of microbial water quality for irrigation can rely not only on concentrations of indicators and/or pathogens, but must include references to crop management. Critical information on microbial composition of actual irrigation waters to support criteria of microbiological quality of irrigation waters appears to be lacking and needs to be collected. C1 [Pachepsky, Yakov; Shelton, Daniel] USDA ARS, Environm Mirobial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. [Dorner, Sarah] Ecole Polytech, Dept Civil Geol & Min Engn, Montreal, PQ H3C 3A7, Canada. [Whelan, Gene] US EPA, Natl Exposure Res Lab, Athens, GA USA. RP Pachepsky, Y (reprint author), USDA ARS, Environm Mirobial & Food Safety Lab, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090 FU United States Environmental Protection Agency through its Office of Research and Development [DW-12-92348101] FX The United States Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under contract DW-12-92348101 to the USDA-ARS. It has been subjected to agency review and approved for publication. NR 78 TC 3 Z9 3 U1 9 U2 18 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1040-841X EI 1549-7828 J9 CRIT REV MICROBIOL JI Crit. Rev. Microbiol. PD MAY PY 2016 VL 42 IS 3 BP 384 EP 393 DI 10.3109/1040841X.2014.954524 PG 10 WC Microbiology SC Microbiology GA DK4OE UT WOS:000374897100004 PM 25198779 ER PT J AU Jones, ZG Goodman, MM Krakowsky, MD AF Jones, Zachary G. Goodman, Major M. Krakowsky, Matthew D. TI Identification of maize-derived dominant gametophyte factors SO EUPHYTICA LA English DT Article DE Dominant gametophyte factors; Plant breeding; Organic agriculture; Coexistence; Ga1-s; Tcb1 AB The use of gametophyte factors to protect specialty-type maize has long been advocated, but as of yet, they have made very little impact on preventing pollen contamination due to the complications associated with breeding with these materials, mainly the additive nature of the alleles. A dominant gametophyte factor (DGF) overcomes this problem, allowing for less time consuming production of gametophytic hybrids, but effectively utilized sources do not exist. Tcb1-s, a known DGF, is a teosinte introgression into maize and the leading candidate for utilization, however, it has several issues that limit its effective use in expediting the breeding process for gametophytic hybrids. The use of maize for a source of DGFs may overcome this problem; with the idea years of selection by farmers would likely have minimized any segregation for yield associated with these alleles, making their use for production of gametophytic hybrids an appealing option for modern breeders. Through screening and backcrossing selected maize accessions, we identified DGFs in seven accessions from race Maiz Dulce, which we document here as a starting point for identification of additional maize-derived DGFs. These accessions did not appear to segregate for yield, a marked improvement over existing DGFs. Additionally, we assessed the compatibility of identified maize-derived DGFs from one accession, and showed that, while lines are generally compatible, they are not obligately so since a single accession may segregate for multiple gametophyte factors. There is, therefore, a need to consider the compatibility of pairs of DGFs early in the inbreeding process. Maize-derived DGFs provide a more effective method of producing gametophytic hybrids, making their production economical enough to be brought to market. The use of DGFs has wider potential to benefit any producers interested in preventing pollen contamination with gametophytic hybrids through the same benefits provided to breeders for organic and other specialty systems. In combination with Ga1-m resistance, maize-derived DGFs provide a long-term gametophytic solution to pollen contamination, in a more expeditious way. C1 [Jones, Zachary G.; Goodman, Major M.] N Carolina State Univ, Dept Crop Sci, 101 Derieux Pl,1236 Williams Hall,Box 7620, Raleigh, NC 27695 USA. [Krakowsky, Matthew D.] N Carolina State Univ, USDA ARS, 101 Derieux Pl,1236 Williams Hall,Box 7620, Raleigh, NC 27695 USA. RP Jones, ZG (reprint author), N Carolina State Univ, Dept Crop Sci, 101 Derieux Pl,1236 Williams Hall,Box 7620, Raleigh, NC 27695 USA. EM zgjones@ncsu.edu FU Clif Bar Family Foundation FX National Institute for Food and Agriculture (NIFA) (#2012-51300-20024). Clif Bar Family Foundation for their support of the Seed Matters Fellowship in Plant Breeding supporting Zachary. Jose de Jesus Sanchez, University of Guadalajara. Jerry Kermicle, University of Wisconsin. NR 13 TC 0 Z9 0 U1 4 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 1 BP 63 EP 69 DI 10.1007/s10681-016-1635-0 PG 7 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK3YU UT WOS:000374854100006 ER PT J AU Hill, ST Coggins, J Liston, A Hendrix, D Henning, JA AF Hill, S. T. Coggins, J. Liston, A. Hendrix, D. Henning, J. A. TI Genomics of the hop pseudo-autosomal regions SO EUPHYTICA LA English DT Article DE Genomics; Humulus; Pseudoautosomal region; Sex chromosomes; Sexual determining region ID HUMULUS-LUPULUS L.; PRIMITIVE Y-CHROMOSOME; SEX-CHROMOSOMES; MOLECULAR MARKERS; REPEAT; PAPAYA; GENE AB Hop is one of the few dioecious plants with dimorphic sex chromosomes. Because the entire Cannabaceae family is dioecious, hop and other members of this family are thought to have a relatively older sex chromosomal system than other plant species. Hop cones are only produced in female hops with or without fertilization. This has lead to most genomic research being directed toward female plants. The work we present provides genomic resources surrounding male plants. We have produced a draft genome for the male hop line USDA 21422M using a novel genome assembly method. In addition, we identified a 1.3 Mb set of scaffolds, which appear to be the male specific region based upon specificity with male hop accessions. This set includes a smaller high confidence total length 18 Kb set of scaffolds, which are supported by over 500 individuals, including the USDA world collection of hop varieties and two mapping populations, with genotyping by sequencing. We also have identified a portion of the Teamaker x 21422M linkage map to be associated with the pseudo-autosomal region (PAR). Within the genomic scaffolds, we identified a set of genes that are sex-linked and likely located in the PAR. C1 [Hill, S. T.; Hendrix, D.] Oregon State Univ, Elect Engn & Comp Sci, Corvallis, OR 97331 USA. [Hendrix, D.] Oregon State Univ, Biochem & Biophys, Corvallis, OR 97331 USA. [Coggins, J.] Roy Farms Inc, Moxie, WA USA. [Liston, A.] Oregon State Univ, Bot & Plant Pathol, Corvallis, OR 97331 USA. [Henning, J. A.] USDA ARS, Forage Seed & Cereal Res, Corvallis, OR 97331 USA. RP Henning, JA (reprint author), USDA ARS, Forage Seed & Cereal Res, Corvallis, OR 97331 USA. EM john.henning@oregonstate.edu OI Henning, John/0000-0002-8310-2066 NR 32 TC 1 Z9 1 U1 2 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 1 BP 171 EP 179 DI 10.1007/s10681-016-1655-9 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK3YU UT WOS:000374854100015 ER PT J AU Baxter, HL Alexander, LW Mazarei, M Haynes, E Turner, GB Sykes, RW Decker, SR Davis, MF Dixon, RA Wang, ZY Stewart, CN AF Baxter, Holly L. Alexander, Lisa W. Mazarei, Mitra Haynes, Ellen Turner, Geoffrey B. Sykes, Robert W. Decker, Stephen R. Davis, Mark F. Dixon, Richard A. Wang, Zeng-Yu Stewart, C. Neal, Jr. TI Hybridization of downregulated-COMT transgenic switchgrass lines with field-selected switchgrass for improved biomass traits SO EUPHYTICA LA English DT Article DE COMT; Switchgrass; Lignocellulosic biofuel; Transgenic; Hybridization ID PANICUM-VIRGATUM L.; LOWLAND SWITCHGRASS; LIGNIN BIOSYNTHESIS; CELL-WALL; OVEREXPRESSION; RECALCITRANCE; FEEDSTOCKS; ETHANOL; YIELD AB Transgenic switchgrass (Panicum virgatum L.) has been produced for improved cell walls for biofuels. For instance, downregulated caffeic acid 3-O-methyltransferase (COMT) switchgrass produced significantly more biomass and biofuel than the non-transgenic progenitor line. In the present study we sought to further improve biomass characteristics by crossing the downregulated COMT T-1 lines with high-yielding switchgrass accessions in two genetic backgrounds ('Alamo' and 'Kanlow'). Crosses and T-2 progeny analyses were made under greenhouse conditions to assess maternal effects, plant morphology and yield, and cell wall traits. Female parent type influenced morphology, but had no effect on cell wall traits. T-2 hybrids produced with T-1 COMT-downregulated switchgrass as the female parent were taller, produced more tillers, and produced 63 % more biomass compared with those produced using the field selected accession as the female parent. Transgene status (presence or absence of transgene) influenced both growth and cell wall traits. T-2 transgenic hybrids were 7 % shorter 80 days after sowing and produced 43 % less biomass than non-transgenic null-segregant hybrids. Cell wall-related differences included lower lignin content, reduced syringyl-to-guaiacyl (S/G) lignin monomer ratio, and a 12 % increase in total sugar release in the T-2 transgenic hybrids compared to non-transgenic null segregants. This is the first study to evaluate the feasibility of transferring the low-recalcitrance traits associated with a transgenic switchgrass line into high-yielding field varieties in an attempt to improve growth-related traits. Our results provide insights into the possible improvement of switchgrass productivity via biotechnology paired with plant breeding. C1 [Baxter, Holly L.; Alexander, Lisa W.; Mazarei, Mitra; Haynes, Ellen; Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. [Baxter, Holly L.; Mazarei, Mitra; Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.; Dixon, Richard A.; Wang, Zeng-Yu; Stewart, C. Neal, Jr.] Oak Ridge Natl Lab, BioEnergy Sci Ctr BESC, Oak Ridge, TN 37831 USA. [Alexander, Lisa W.] USDA ARS, Otis L Floyd Nursery Res Ctr, US Natl Arboretum, McMinnville, TN 37110 USA. [Turner, Geoffrey B.; Sykes, Robert W.; Decker, Stephen R.; Davis, Mark F.] Natl Renewable Energy Lab, Golden, CO 80401 USA. [Dixon, Richard A.] Univ N Texas, Dept Biol Sci, Denton, TX 76203 USA. [Wang, Zeng-Yu] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. RP Stewart, CN (reprint author), Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. EM nealstewart@utk.edu OI davis, mark/0000-0003-4541-9852 FU Agriculture and Food Research Initiative (United States Department of Agriculture); Southeastern Partnership for Integrated Biomass and Supply Systems (The IBSS Partnership); Office of Biological and Environmental Research in the DOE Office of Science FX We thank Fred Allen, Hem Bhandari, and Ken Goddard for providing the Tennessee field accessions of switchgrass field selections, and Justin Leduc and Erika Barton for their assistance with collecting and preparing samples for cell wall characterization analyses. This work was supported by the Agriculture and Food Research Initiative (United States Department of Agriculture) and Southeastern Partnership for Integrated Biomass and Supply Systems (The IBSS Partnership), and enabled by the Bioenergy Science Center. The Bioenergy Science Center is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. NR 24 TC 0 Z9 0 U1 1 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 2 BP 341 EP 355 DI 10.1007/s10681-016-1632-3 PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK6VU UT WOS:000375064500005 ER PT J AU Robbins, MD Staub, JE Bushman, BS AF Robbins, Matthew D. Staub, Jack E. Bushman, B. Shaun TI Development of fine-leaved Festuca grass populations identifies genetic resources having improved forage production with potential for wildfire control in the western United States SO EUPHYTICA LA English DT Article DE Abiotic tolerance; AFLP; Breeding; Environmental stress; Germplasm enhancement; Molecular markers ID STRESS; PLANTS; SIMULATION; RESPONSES; SOFTWARE; POACEAE; FIRE; AFLP; DNA AB Drought and heat tolerant fine-leaved fescue (Festuca ssp.) grasses have potential as components in rangeland greenstrips for wildfire control in semi-arid climates, although such grasses have not been evaluated under rangeland conditions. Therefore, 63 geographically diverse Festuca accessions of 11 species were evaluated for vigor, color, and biomass in 2009 and 2010 in North Logan, UT to identify grasses for use in U.S. western rangelands. Sixty-two plants representing eight species were selected in 2009 to intermate for further evaluation. Controlled biparental matings among these selections in 2010 produced 18 populations with sufficient seed to be evaluated with three commercial Festuca checks in replicated trials between 2012 and 2013 at Malta, ID, Blue Creek, UT, and North Logan, UT, where mean annual precipitation is 265, 362, and 484 mm, respectively. Plants were evaluated for color, relative vigor, biomass, seed yield, persistence, and regrowth over 2 years. Generally, four fine-leaved populations (R4S4, R4S6, R4S22, and R4S32) with parents originating from Turkey (F. valesiaca subsp. valesiaca), Russia (F. valesiaca, F. valesiaca subsp. valesiaca), Iran (F. valesiaca), and the U.S. (F. ovina) performed equal to or better than 'Durar' or 'Covar' checks. In Malta (harshest environment), the performance of these four populations compared to 'Durar' was 84-210 % for vigor, 79-90 % for color, 65-562 % for biomass, 64-296 % for seed yield, 92-117 % for persistence, and 164-454 % for regrowth, where R4S22 was superior. AFLP analysis indicated that all four populations were distinct, and that R4S4 and R4S6 grouped near 'Covar', R4S22 clustered near 'Black Sheep' and 'Durar', and R4S32 was genetically unique. These populations exhibit drought tolerance and green leaf color under harsh U.S. western desert conditions that make them amendable for use in greenstrips for wildfire control. C1 [Robbins, Matthew D.; Staub, Jack E.; Bushman, B. Shaun] Utah State Univ, USDA ARS, Forage & Range Res Lab, 696 N 1100 E, Logan, UT 84322 USA. RP Robbins, MD (reprint author), Utah State Univ, USDA ARS, Forage & Range Res Lab, 696 N 1100 E, Logan, UT 84322 USA. EM matthew.robbins@ars.usda.gov NR 49 TC 0 Z9 0 U1 5 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 2 BP 377 EP 393 DI 10.1007/s10681-016-1644-z PG 17 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK6VU UT WOS:000375064500007 ER PT J AU Chen, CX Bai, JH Okie, WR Plotto, A AF Chen, Chunxian Bai, Jinhe Okie, William R. Plotto, Anne TI Comparison of fruit characters and volatile components in peach-to-nectarine mutants SO EUPHYTICA LA English DT Article DE Bud sport; Pleiotropic effect; Spontaneous mutant; Total ion current; Volatile organic compound ID GENOME AB Peach-to-nectarine mutants showed broad pleiotropic effects on fruit size, taste, and aroma, in addition to hairlessness. In this study, we compared nine fruit attributes and 27 detected volatiles in the peach progenitor, 'Flameprince' (FPP), its two independently discovered peach-to-nectarine mutants (HFN and PFN), and a selected nectarine hybrid (SLN). HFN and PFN differed from FPP in fruit size and taste, but shared high similarities in fruit blush coverage and pattern, pit shape, and flesh and pit color. Compared to FPP, the two mutants were significantly lighter and smaller, but higher in soluble solids content (SSC), titratable acidity (TA), and the SSC/TA ratio. The 27 volatiles varied among the cultivars and harvest dates, in terms of total abundance and detection rate, and could be roughly clustered into six subgroups. Subgroup 1 included five volatiles and two fruit attributes that were low on Aug 6 but dramatically increased on Aug 13. Subgroup 2 had a similar tendency to subgroup 1 but one of the cultivars was an exception. Subgroup 3 included those that had the opposite tendency to subgroup 1 or 2. Volatiles in the three subgroups appeared closely associated with the ripening process. Fruit volatiles and attributes in the other three subgroups generally were inconsistent among the four cultivars on the two dates. Hexanol was the only volatile undetected in the peach, but detected in the three nectarines. Differences in broad pleiotropic effects among these mutants, potential utilization of volatiles, and genomic exploration are discussed. C1 [Chen, Chunxian; Okie, William R.] ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. [Bai, Jinhe; Plotto, Anne] ARS, USDA, Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP Chen, CX (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM chunxian.chen@ars.usda.gov FU USDA National Program of Plant Genetic Resources, Genomics and Genetic Improvement [6606-21000-004-006]; Georgia Agriculture Commodity Commission for Peaches [58-6042-5-002] FX The authors thank Dr. Elizabeth Baldwin for her useful suggestions and Bryan Blackburn and Luke Quick for their technical assistance. The research is partially supported by the USDA National Program of Plant Genetic Resources, Genomics and Genetic Improvement (Project number: 6606-21000-004-006) and Georgia Agriculture Commodity Commission for Peaches (Project number: 58-6042-5-002). This article reports the results of research only. Mention of a trademark or proprietary product is solely for the purpose of providing specific information and does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable. NR 21 TC 0 Z9 0 U1 10 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 2 BP 409 EP 418 DI 10.1007/s10681-016-1648-8 PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK6VU UT WOS:000375064500009 ER PT J AU McCord, PH AF McCord, Per H. TI Using droplet digital PCR (ddPCR) to detect copy number variation in sugarcane, a high-level polyploid SO EUPHYTICA LA English DT Article DE Copy number variation; Digital PCR; Dosage analysis; Polyploidy ID QUANTITATIVE PCR; GENOME-WIDE; DNA; GENE; EXPRESSION; REVEALS; DISEASE AB Copy number variation (CNV) generally refers to duplications or deletions of sections of DNA, including genes. In polyploid plant species, CNV can also take the form of multiple copies of an allele at a given locus. This variation in dosage has been shown to alter gene expression and phenotype. As copy number rises, it becomes increasingly challenging to quantify dosage changes. A new technique, known as droplet digital PCR (ddPCR), has been used for measuring CNV. Digital PCR partitions a PCR reaction into many thousands to millions of discrete reactions (emulsified droplets in the case of ddPCR). This partitioning has the effect of diluting the target DNA, such that some droplets contain zero copies of the target, while others contain one or more copies. Post-PCR, this results in a binary (positive or negative) outcome, hence the term digital. Partitioning the reaction into a large number of discrete tests also increases sensitivity and dynamic range. In this article, ddPCR was employed to survey sugarcane (Saccharum spp.) germplasm for dosage variation for the Bru1 locus, which imparts resistance to the fungal disease known as brown rust. The breeding nursery at the USDA-ARS Sugarcane Field Station in Canal Point, FL was surveyed for the presence of Bru1 using standard methods. Those clones which were Bru1-positive were then analyzed via ddPCR. Out of 80 Bru1-positive clones, 60 were simplex or single copy. Eighteen clones were duplex for Bru1, and there were two clones that were triplex. The progeny of several testcrosses supported the results of ddPCR; a simplex parent produced approximately 50 % positive progeny, while parents that were duplex produced more than 80 %. Further verification was done via quantitative PCR. Results generally agreed with ddPCR, in that three groups could be distinguished, but the groups were relative, and not quantitative. This analysis of dosage variation has important implications for both breeding and molecular biology research. Clones with more than one copy of Bru1 (or any other gene of interest) will pass on the gene at much higher frequencies than clones that are simplex. In addition, the identification of clones with varying gene dosages allows for formal testing of dosage effects. This is the first report of ddPCR being used to measure copy number/dosage variation in a high-level polyploid plant species. C1 [McCord, Per H.] USDA ARS, Sugarcane Field Stn, 12990 Highway 441 N, Canal Point, FL 33438 USA. RP McCord, PH (reprint author), USDA ARS, Sugarcane Field Stn, 12990 Highway 441 N, Canal Point, FL 33438 USA. EM Per.mccord@ars.usda.gov NR 31 TC 0 Z9 0 U1 7 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 2 BP 439 EP 448 DI 10.1007/s10681-016-1657-7 PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK6VU UT WOS:000375064500012 ER PT J AU Arro, J Park, JW Wai, CM VanBuren, R Pan, YB Nagai, C da Silva, J Ming, R AF Arro, Jie Park, Jong-Won Wai, Ching Man VanBuren, Robert Pan, Yong-Bao Nagai, Chifumi da Silva, Jorge Ming, Ray TI Balancing selection contributed to domestication of autopolyploid sugarcane (Saccharum officinarum L.) SO EUPHYTICA LA English DT Article DE Domestication; Polyploid; Population genetics; Selection scan; Sugarcane ID SYNTHASE GENE FAMILY; POPULATION GENOMICS; ARTIFICIAL SELECTION; NATURAL-SELECTION; SORGHUM-BICOLOR; SEQUENCING DATA; RNA-SEQ; MARKERS; DIVERSITY; MAIZE AB Sugarcane was domesticated in New Guinea about 10,000 years ago, and the domesticated species Saccharum officinarum is autooctoploid. For diploid domesticated crops, genetic diversity is reduced for those genes controlling favorable traits. However, the domestication traits in sugarcane such as sugar content and biomass yield are controlled by multiple genes with multiple alleles. A genomics approach to identify genes involved in the transition from wild to domesticated may provide useful insight into complex polyploid traits such as sucrose accumulation. Fifteen accessions each of domesticated S. officinarum and the wild species S. robustum and eighteen accessions of S. spontaneum were used for sequencing of leaf and stalk transcriptomes. We found high allelic diversity among genes expressed in the stalk tissues where the domesticated S. officinarum (F-is = 0.69) surprisingly has higher allele diversity than its wild relative S. spontaneum (F-is = 0.41). However, there were no SNP loci with extremely high FST values despite the observed higher average FST among the three species, indicative of the action of balancing selection. This is corroborated by nucleotide diversity and site frequency spectrum (SFS) patterns that show that majority of expressed genes in S. officinarum have comparable per-site heterozygosity to the wild species. These candidate domestication genes, bearing signatures of balancing selection and excess singleton SNPs in S. officinarum, perturbs pathways involved in sucrose and starch metabolism. C1 [Arro, Jie; Wai, Ching Man; VanBuren, Robert; Ming, Ray] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Park, Jong-Won] Texas A&M Univ Kingsville, Citrus Ctr, Weslaco, TX 78599 USA. [Pan, Yong-Bao] USDA ARS, Houma, LA USA. [Nagai, Chifumi] Hawaii Agr Res Ctr, Kunia, HI 96759 USA. [da Silva, Jorge] Texas A&M Univ Syst, Texas A&M AgriLife Res, Weslaco, TX 78599 USA. [Ming, Ray] Fujian Agr & Forestry Univ, FAFU & UIUC SIB Joint Ctr Genom & Biotechnol, Fuzhou 350002, Fujian, Peoples R China. RP Ming, R (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM rming@life.uiuc.edu FU International Consortium for Sugarcane Biotechnology; EBI [BP2012OO2J17]; Texas Governor's Office Emerging Technology Funds, Bioenergy; US DOE [DE-SC0010686] FX This project was supported by grants from the International Consortium for Sugarcane Biotechnology, EBI BP2012OO2J17, the Texas Governor's Office Emerging Technology Funds, Bioenergy, and US DOE DE-SC0010686. NR 56 TC 0 Z9 0 U1 5 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD MAY PY 2016 VL 209 IS 2 BP 477 EP 493 DI 10.1007/s10681-016-1672-8 PG 17 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DK6VU UT WOS:000375064500015 ER PT J AU Otoni, CG Espitia, PJP Avena-Bustillos, RJ McHugh, TH AF Otoni, Caio G. Espitia, Paula J. P. Avena-Bustillos, Roberto J. McHugh, Tara H. TI Trends in antimicrobial food packaging systems: Emitting sachets and absorbent pads SO FOOD RESEARCH INTERNATIONAL LA English DT Review DE Active packaging; Antimicrobial sachets; Absorbent pads; Volatile antimicrobials; Antimicrobial nanoparticles ID SHELF-LIFE EXTENSION; SPOILAGE-RELATED MICROFLORA; ESCHERICHIA-COLI O157-H7; OREGANO ESSENTIAL OIL; PLANT ESSENTIAL OILS; ALLYL-ISOTHIOCYANATE; LISTERIA-MONOCYTOGENES; CHLORINE DIOXIDE; MODIFIED ATMOSPHERE; ANTIBACTERIAL ACTIVITY AB Active antimicrobial packaging interacts with packaged food and headspace to reduce, retard, or even inhibit the growth of spoilage and pathogenic microorganisms. Sachets and pads are one of the most successful applications of active food packaging. This review discusses recent developments of antimicrobial active packaging focused exclusively on emitting sachets and absorbent pads, including elaboration techniques, characterization methods, and applications for food preservation purposes. Advantages, drawbacks, and future trends are also discussed, as well as the antimicrobial compounds incorporated in emitting sachets and absorbent pads, including ethanol, chlorine dioxide, a variety of plant essential oils and their main active compounds, and nanoparticles. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Otoni, Caio G.] Univ Fed Sao Carlos, Dept Mat Engn, PPG CEM, Rod Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, Brazil. [Otoni, Caio G.] Embrapa Instrumentacao, Natl Nanotechnol Lab Agribusiness, Rua 15 Novembro 1452, BR-13560979 Sao Carlos, SP, Brazil. [Espitia, Paula J. P.] Observ Caribe Colombiano Res Inst, Food Res Div, Calle Guerrero 29-02, Cartagena De Indias, Colombia. [Espitia, Paula J. P.] Univ Sinu, Nutr & Dietet Sch, Food Sci Lab, Cartagena De Indias, Colombia. [Avena-Bustillos, Roberto J.; McHugh, Tara H.] ARS, Hlth Proc Foods Res, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. RP Otoni, CG (reprint author), Embrapa Instrumentacao, Natl Nanotechnol Lab Agribusiness, Rua 15 Novembro 1452, BR-13560979 Sao Carlos, SP, Brazil. EM caiogomide@gmail.com; perez.espitia@gmail.com; roberto.avena@ars.usda.gov; tara.mchugh@ars.usda.gov RI Otoni, Caio/F-6743-2012 OI Otoni, Caio/0000-0001-6734-7381 FU Sao Paulo Research Foundation (FAPESP) [2014/23098-9]; COLCIENCIAS FX CG Otoni and PJP Espitia acknowledge the funding provided by Sao Paulo Research Foundation (FAPESP, grant #2014/23098-9) and COLCIENCIAS, respectively. FST is also acknowledged for inspiring this review and making the time for bibliographical research available. NR 97 TC 11 Z9 11 U1 20 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0963-9969 EI 1873-7145 J9 FOOD RES INT JI Food Res. Int. PD MAY PY 2016 VL 83 BP 60 EP 73 DI 10.1016/j.foodres.2016.02.018 PG 14 WC Food Science & Technology SC Food Science & Technology GA DK3EZ UT WOS:000374801100006 ER PT J AU Larsen, AL Jacquot, JJ Keenlance, PW Keough, HL AF Larsen, Angela L. Jacquot, Joseph J. Keenlance, Paul W. Keough, Heather L. TI Effects of an ongoing oak savanna restoration on small mammals in Lower Michigan SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Small mammals; Thinning; Oak savanna; Quercus spp.; Habitat restoration; Michigan ID COARSE WOODY DEBRIS; BIRD COMMUNITIES; SEED DISPERSAL; PINE FOREST; OLD FIELDS; FIRE; GRASSLAND; RESPONSES; POPULATIONS; RODENTS AB Oak (Quercus spp.) savannas have declined drastically in the midwestern United States since European settlement. Oak savanna restoration projects are primarily driven by species closely linked to this habitat type, such as the federally endangered Karner blue butterfly (Lycaeides melissa samuelis). However, it is essential that other species are monitored during restoration. Small mammals, due to their importance in ecosystem function, are particularly useful to study. The United States Forest Service is currently conducting an oak savanna restoration in the Manistee National Forest in Lower Michigan using forest thinning and prescribed burning. To understand management impacts on small mammal communities, we live trapped small mammals in each of the mechanically thinned plots (i.e., bulldozer, masticator, and shear cutter) and control plots in five blocks over six years (2008-2013), as well as measured vegetation variables each year. Initially, we used a permutation multivariate analysis of variance (perMANOVA) to determine if there were treatment and year interactions for both small mammal community assemblages and vegetation variables. We then compared changes in small mammal diversity, relative abundance, and vegetation variables among treatments using exploratory randomized block design analysis of variances (ANOVAs). Canopy cover was significantly lower in bulldozer and shear cutter thinned plots than control plots five years following thinning. We observed significant treatment by year interactions in how the small mammal community responded. A large increase in relative abundance of white-footed mice occurred one year post-thinning in all treatments. Within 1-2 years of treatment, thirteen-lined ground squirrels and meadow jumping mice, both open-canopy grassland species became established on thinned plots. The retention of brush piles in bulldozer and shear cutter plots provided important refuge habitat for small mammals following thinning, Restoration efforts were beneficial to the small mammal community overall and promoted grassland species to immigrate into the restored area. (C) 2016 Elsevier B.V. All rights reserved. C1 [Larsen, Angela L.; Jacquot, Joseph J.; Keenlance, Paul W.] Grand Valley State Univ, Dept Biol, 1 Campus Dr,3300a Kindschi Hall Sci, Allendale, MI 49401 USA. [Keough, Heather L.] US Forest Serv, Huron Manistee Natl Forests, 605 N Michigan Ave, Baldwin, MI 49304 USA. [Larsen, Angela L.] Univ N Carolina, Dept Biol, 312 Eberhart Bldg, Greensboro, NC 27402 USA. RP Larsen, AL (reprint author), Grand Valley State Univ, Dept Biol, 1 Campus Dr,3300a Kindschi Hall Sci, Allendale, MI 49401 USA.; Larsen, AL (reprint author), Univ N Carolina, Dept Biol, 312 Eberhart Bldg, Greensboro, NC 27402 USA. EM allarsen@uncg.edu FU Grand Valley State University; Michigan Chapter of Safari Club International FX We thank Grand Valley State University and the Michigan Chapter of Safari Club International for providing funding for this research and the USFS Baldwin/White Cloud Ranger Station for providing equipment and housing. We would also like to thank the USFS technicians and volunteers for collecting the vegetation data and S. Miara, T. Hillman, and all of the other field volunteers. NR 48 TC 0 Z9 0 U1 16 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2016 VL 367 BP 120 EP 127 DI 10.1016/j.foreco.2016.02.016 PG 8 WC Forestry SC Forestry GA DK8EZ UT WOS:000375161300013 ER PT J AU Lee, JY Beom, HR Altenbach, SB Lim, SH Kim, YT Kang, CS Yoon, UH Gupta, R Kim, ST Ahn, SN Kim, YM AF Lee, Jong-Yeol Beom, Hye-Rang Altenbach, Susan B. Lim, Sun-Hyung Kim, Yeong-Tae Kang, Chon-Sik Yoon, Ung-Han Gupta, Ravi Kim, Sun-Tae Ahn, Sang-Nag Kim, Young-Mi TI Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety SO FUNCTIONAL & INTEGRATIVE GENOMICS LA English DT Article DE Triticum aestivum L.; LMW-GS genes; Proteomics; Wheat quality improvement ID WEIGHT GLUTENIN SUBUNITS; TANDEM MASS-SPECTROMETRY; BREAD-MAKING QUALITY; TRITICUM-AESTIVUM L.; 2-DIMENSIONAL GEL-ELECTROPHORESIS; STS MARKERS; SDS-PAGE; MOLECULAR CHARACTERIZATION; GLU-A3 ALLELES; ENDOSPERM AB Although it is well known that low-molecular-weight glutenin subunits (LMW-GS) from wheat affect bread and noodle processing quality, the function of specific LMW-GS proteins remains unclear. It is important to find the genes that correspond to individual LMW-GS proteins in order to understand the functions of specific proteins. The objective of this study was to link LMW-GS genes and haplotypes characterized using well known Glu-A3, Glu-B3, and Glu-D3 gene-specific primers to their protein products in a single wheat variety. A total of 36 LMW-GS genes and pseudogenes were amplified from the Korean cultivar Keumkang. These include 11 Glu-3 gene haplotypes, two from the Glu-A3 locus, two from the Glu-B3 locus, and seven from the Glu-D3 locus. To establish relationships between gene haplotypes and their protein products, a glutenin protein fraction was separated by two-dimensional gel electrophoresis (2-DGE) and 17 protein spots were analyzed by N-terminal amino acid sequencing and tandem mass spectrometry (MS/MS). LMW-GS proteins were identified that corresponded to all Glu-3 gene haplotypes except the pseudogenes. This is the first report of the comprehensive characterization of LMW-GS genes and their corresponding proteins in a single wheat cultivar. Our approach will be useful to understand the contributions of individual LMW-GS to the end-use quality of flour. C1 [Lee, Jong-Yeol; Beom, Hye-Rang; Lim, Sun-Hyung; Yoon, Ung-Han; Kim, Young-Mi] RDA, Natl Acad Agr Sci, Jeonju 560500, South Korea. [Altenbach, Susan B.] USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Kim, Yeong-Tae] Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. [Kang, Chon-Sik] RDA, Natl Inst Crop Sci, Jeonju 565851, South Korea. [Gupta, Ravi; Kim, Sun-Tae] Pusan Natl Univ, Dept Plant Biosci, Miryang 627706, South Korea. [Ahn, Sang-Nag] Chungnam Natl Univ, Dept Agron, Daejeon 357764, South Korea. RP Lee, JY; Kim, YM (reprint author), RDA, Natl Acad Agr Sci, Jeonju 560500, South Korea. EM jy0820@korea.kr; ymikim@korea.kr FU National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ010116] FX This work was supported by grants from the National Academy of Agricultural Science (PJ010116), Rural Development Administration, Republic of Korea. NR 40 TC 3 Z9 3 U1 9 U2 22 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1438-793X EI 1438-7948 J9 FUNCT INTEGR GENOMIC JI Funct. Integr. Genomics PD MAY PY 2016 VL 16 IS 3 BP 269 EP 279 DI 10.1007/s10142-016-0482-3 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA DL1ST UT WOS:000375413200005 PM 26882917 ER PT J AU Li, XB Yan, WG Agrama, H Jackson, A Jia, M Jia, LM Moldenhauer, K Correa, F Wu, DX AF Li, Xiaobai Yan, Wengui Agrama, Hesham Jackson, Aaron Jia, Melissa Jia, Limeng Moldenhauer, Karen Correa, Fernando Wu, Dianxing TI Genetic analysis of genetic basis of a physiological disorder "straighthead" in rice (Oryza sativa L.) SO GENES & GENOMICS LA English DT Article DE Rice; Straighthead; Germplasm; Linkage mapping ID CULTIVARS; MSMA; STERILITY; MINERALS AB Straighthead is a physiological disorder in rice that causes yield losses and is a serious threat to rice production worldwide. Identification of QTL conferring resistance will help develop resistant cultivars for straighthead control. We conducted linkage mapping to identify QTL involved with straighthead. The study was based on a F-2 population developed from a cross between 'Zhe733(resistant)/R312(susceptible)'. Using phenotypic data of F-2 plants and their F-2:3 families, two major QTL, qSTH-2 and qSTH-8, were identified using bulked segregant analysis, explaining 11.1 and 28.1 % of the phenotypic variation on chromosome 2 and 8, respectively. The qSTH-2 for straighthead resistance was identified by linkage mapping. qSTH-2 was situated near a QTL "AsS" responsible for arsenic accumulation. Straighthead is frequently observed on land where As has accumulated. The result suggests a kind of internal connection between qSTH-2 and AsS. Additionally, the QTL qSTH-8 was located close to HD5 related with heading date. The close location may be associated with the observation of early heading among straighthead resistant varieties. These findings should be useful for further genetic study of straighthead. C1 [Li, Xiaobai; Jia, Limeng; Wu, Dianxing] Zhejiang Univ, IAEA Collaborating Ctr, State Key Lab Rice Biol, Hangzhou 310003, Zhejiang, Peoples R China. [Yan, Wengui; Jackson, Aaron; Jia, Melissa] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Li, Xiaobai; Jia, Limeng] ZheJiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China. [Agrama, Hesham; Moldenhauer, Karen] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR USA. [Correa, Fernando] RiceTec Inc, Alvin, TX USA. RP Li, XB; Wu, DX (reprint author), Zhejiang Univ, IAEA Collaborating Ctr, State Key Lab Rice Biol, Hangzhou 310003, Zhejiang, Peoples R China.; Li, XB (reprint author), ZheJiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China. EM hufanfan1982815@gmail.com; dxwu@zju.edu.cn FU Chinese 973 program [2013CBA01405]; zhejiang provincial Breeding program [0406] FX This research was supported by the Chinese 973 program (2013CBA01405) and zhejiang provincial Breeding program (0406). NR 30 TC 1 Z9 1 U1 1 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1976-9571 EI 2092-9293 J9 GENES GENOM JI Genes Genom. PD MAY PY 2016 VL 38 IS 5 BP 453 EP 457 DI 10.1007/s13258-016-0394-6 PG 5 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA DK4TN UT WOS:000374912600007 ER PT J AU Li, XB Yan, WG Agrama, H Jackson, A Jia, M Jia, LM Moldenhauer, K Correa, F Wu, DX AF Li, Xiaobai Yan, Wengui Agrama, Hesham Jackson, Aaron Jia, Melissa Jia, Limeng Moldenhauer, Karen Correa, Fernando Wu, Dianxing TI Genetic analysis of genetic basis of a physiological disorder "straighthead" in rice (Oryza sativa L.) (vol 38, pg 453, 2016) SO GENES & GENOMICS LA English DT Correction C1 [Li, Xiaobai; Jia, Limeng; Wu, Dianxing] Zhejiang Univ, IAEA Collaborating Ctr, State Key Lab Rice Biol, Hangzhou 310003, Zhejiang, Peoples R China. [Yan, Wengui; Jackson, Aaron; Jia, Melissa] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Li, Xiaobai; Jia, Limeng] Zhejiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China. [Agrama, Hesham; Moldenhauer, Karen] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR USA. [Correa, Fernando] RiceTec Inc, Alvin, TX USA. [Agrama, Hesham] Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Crop Sci, POB 34, Al Khoud 123, Oman. RP Li, XB; Wu, DX (reprint author), Zhejiang Univ, IAEA Collaborating Ctr, State Key Lab Rice Biol, Hangzhou 310003, Zhejiang, Peoples R China.; Li, XB (reprint author), Zhejiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China. EM hufanfan1982815@gmail.com; dxwu@zju.edu.cn NR 1 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1976-9571 EI 2092-9293 J9 GENES GENOM JI Genes Genom. PD MAY PY 2016 VL 38 IS 5 BP 489 EP 489 DI 10.1007/s13258-016-0403-9 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA DK4TN UT WOS:000374912600011 ER PT J AU Dimitrov, KM Lee, DH Williams-Coplin, D Olivier, TL Miller, PJ Afonso, CL AF Dimitrov, Kiril M. Lee, Dong-Hun Williams-Coplin, Dawn Olivier, Timothy L. Miller, Patti J. Afonso, Claudio L. TI Newcastle Disease Viruses Causing Recent Outbreaks Worldwide Show Unexpectedly High Genetic Similarity to Historical Virulent Isolates from the 1940s SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID MULTIPLE SEQUENCE ALIGNMENT; AVIAN PARAMYXOVIRUS TYPE-1; FUSION PROTEIN GENE; WILD BIRDS; MOLECULAR EPIDEMIOLOGY; F-GENE; GENOTYPE; STRAINS; DIVERSITY; POULTRY AB Virulent strains of Newcastle disease virus (NDV) cause Newcastle disease (ND), a devastating disease of poultry and wild birds. Phylogenetic analyses clearly distinguish historical isolates (obtained prior to 1960) from currently circulating viruses of class II genotypes V, VI, VII, and XII through XVIII. Here, partial and complete genomic sequences of recent virulent isolates of genotypes II and IX from China, Egypt, and India were found to be nearly identical to those of historical viruses isolated in the 1940s. Phylogenetic analysis, nucleotide distances, and rates of change demonstrate that these recent isolates have not evolved significantly from the most closely related ancestors from the 1940s. The low rates of change for these virulent viruses (7.05 x 10(-5) and 2.05 x 10(-5) per year, respectively) and the minimal genetic distances existing between these and historical viruses (0.3 to 1.2%) of the same genotypes indicate an unnatural origin. As with any other RNA virus, Newcastle disease virus is expected to evolve naturally; thus, these findings suggest that some recent field isolates should be excluded from evolutionary studies. Furthermore, phylogenetic analyses show that these recent virulent isolates are more closely related to virulent strains isolated during the 1940s, which have been and continue to be used in laboratory and experimental challenge studies. Since the preservation of viable viruses in the environment for over 6 decades is highly unlikely, it is possible that the source of some of the recent virulent viruses isolated from poultry and wild birds might be laboratory viruses. C1 [Dimitrov, Kiril M.; Lee, Dong-Hun; Williams-Coplin, Dawn; Olivier, Timothy L.; Miller, Patti J.; Afonso, Claudio L.] ARS, SE Poultry Res Lab, USDA, Athens, GA USA. RP Afonso, CL (reprint author), ARS, SE Poultry Res Lab, USDA, Athens, GA USA. EM claudio.afonso@ars.usda.gov FU USDA \ Agricultural Research Service (ARS) [6040-32000-064]; DOD \ Defense Threat Reduction Agency (DTRA) [FRCALL12-6-2-0005] FX This work, including the efforts of Dawn Williams-Coplin, Timothy L Olivier, Patti J Miller, and Claudio L Afonso, was funded by USDA vertical bar Agricultural Research Service (ARS) (6040-32000-064). This work, including the efforts of Dong-Hun Lee, was funded by USDA vertical bar Agricultural Research Service (ARS). This work, including the efforts of Kiril M Dimitrov, was funded by DOD vertical bar Defense Threat Reduction Agency (DTRA) (FRCALL12-6-2-0005). NR 56 TC 1 Z9 1 U1 3 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAY PY 2016 VL 54 IS 5 BP 1228 EP 1235 DI 10.1128/JCM.03044-15 PG 8 WC Microbiology SC Microbiology GA DK5JH UT WOS:000374955700012 PM 26888902 ER PT J AU Feyereisen, GW Moorman, TB Christianson, LE Venterea, RT Coulter, JA Tschirner, UW AF Feyereisen, Gary W. Moorman, Thomas B. Christianson, Laura E. Venterea, Rodney T. Coulter, Jeffrey A. Tschirner, Ulrike W. TI Performance of Agricultural Residue Media in Laboratory Denitrifying Bioreactors at Low Temperatures SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NITROUS-OXIDE REDUCTASE; NITRATE REMOVAL; DENITRIFICATION BIOREACTORS; MICROBIAL COMMUNITY; WHEAT-STRAW; DRAINAGE; WATER; SOILS; GENE AB Denitrifying bioreactors can be effective for removing nitrate from agricultural tile drainage; however, questions about cold springtime performance persist. The objective of this study was to improve the nitrate removal rate (NRR) of denitrifying bioreactors at warm and cold temperatures using agriculturally derived media rather than wood chips (WC). Corn (Zea mays L.) cobs (CC), corn stover (CS), barley (Hordeum vulgare L.) straw (BS), WC, and CC followed by a compartment of WC (CC+WC) were tested in laboratory columns for 5 mo at a 12-h hydraulic residence time in separate experiments at 15.5 and 1.5 degrees C. Nitrate-N removal rates ranged from 35 to 1.4 at 15.5 degrees C and from 7.4 to 1.6 g N m(-3) d(-1) at 1.5 degrees C, respectively; NRRs were ranked CC > CC+WC > BS = CS > WC and CC = CC+WC = CS = BS > WC for 15.5 and 1.5 degrees C, respectively. Although NRRs for CC were increased relative to WC, CC released greater amounts of carbon. Greater abundance of nitrous oxide (N2O) reductase gene (nosZ) was supported by crop residues than WC at 15.5 degrees C, and CS and BS supported greater abundance than WC at 1.5 degrees C. Production of N2O relative to nitrate removal (rN(2)O) was consistently greater at 1.5 degrees C (7.5% of nitrate removed) than at 15.5 degrees C (1.9%). The rN(2)O was lowest in CC (1.1%) and CC-WC (0.9%) and greatest in WC (9.7%). Using a compartment of agricultural residue media in series before wood chips has the potential to improve denitrifying bioreactor nitrate removal rates, but field-scale verification is needed. C1 [Feyereisen, Gary W.; Venterea, Rodney T.] USDA ARS, Soil & Water Management Res Unit, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Moorman, Thomas B.] USDA ARS, Natl Lab Agr & Environm, 1015 N Univ Blvd, Ames, IA 50011 USA. [Christianson, Laura E.] Univ Illinois, Dept Crop Sci, 1102 S Goodwin Ave, Urbana, IL 61801 USA. [Coulter, Jeffrey A.] Univ Minnesota, Dept Agron & Plant Genet, 411 Bourlag Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Tschirner, Ulrike W.] Univ Minnesota, Dept Bioprod & Biosyst Engn, 2004 Folwell Ave, St Paul, MN 55108 USA. RP Feyereisen, GW (reprint author), USDA ARS, Soil & Water Management Res Unit, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. EM gary.feyereisen@ars.usda.gov FU AURI; Minnesota Corn Research and Promotion Council; USDA-ARS [59-1930-0-046] FX The authors thank Dr. Debra Palmquist, Statistician General, USDA-ARS Midwest Area, for her contribution to the experimental design; Todd Schumacher, Biological Science Technician, USDA-ARS St. Paul, MN, for overseeing experimental operations; Taylor Hoffman, Liz Petesch, and Noah Slocum for tending the experiment; University of Minnesota for providing facilities; Ranae Jorgenson, Analytical Chemist, Minnesota Agricultural Utilization Research Institute (AURI), for analyzing material properties; and Alan Doering and Becky Philipp, AURI, for providing project organization. This work was supported by AURI and the Minnesota Corn Research and Promotion Council. Coauthor L.E. Christianson's time was supported through USDA-ARS Agreement No. 59-1930-0-046. NR 40 TC 4 Z9 4 U1 11 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 779 EP 787 DI 10.2134/jeq2015.07.0407 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700004 PM 27136142 ER PT J AU Hoover, NL Bhandari, A Soupir, ML Moorman, TB AF Hoover, Natasha L. Bhandari, Alok Soupir, Michelle L. Moorman, Thomas B. TI Woodchip Denitrification Bioreactors: Impact of Temperature and Hydraulic Retention Time on Nitrate Removal SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID MISSISSIPPI RIVER; DRAINAGE WATER; NITROGEN; LOSSES; SYSTEMS; SOIL; RESPIRATION; MANAGEMENT; TRANSPORT; MANURE AB Woodchip denitrification bioreactors, a relatively new technology for edge-of-field treatment of subsurface agricultural drainage water, have shown potential for nitrate removal. However, few studies have evaluated the performance of these reactors under varied controlled conditions including initial woodchip age and a range of hydraulic retention times (HRTs) and temperatures similar to the field. This study investigated (i) the release of total organic C (TOC) during reactor start up for fresh and weathered woodchips, (ii) nitrate (NO3-N) removal at HRTs ranging from 2 to 24 h, (iii) nitrate removal at influent NO3-N concentrations of 10, 30, and 50 mg L-1, and (iv) NO3-N removal at 10, 15, and 20 degrees C. Greater TOC was released during bioreactor operation with fresh woodchips, whereas organic C release was low when the columns were packed with naturally weathered woodchips. Nitrate-N concentration reductions increased from 8 to 55% as HRT increased. Nitrate removal on a mass basis (g NO3-N m(-3) d(-1)) did not follow the same trend, with relatively consistent mass removal measured as HRT increased from 1.7 to 21.2 h. Comparison of mean NO3-N load reduction for various influent NO3-N concentrations showed lower reduction at an influent concentration of 10 mg L-1 and higher NO3-N reductions at influent concentrations of 30 and 50 mg L-1. Nitrate-N removal showed a stepped increase with temperature. Temperature coefficient (Q(10)) factors calculated from NO3-N removal rates ranged from 2.2 to 2.9. C1 [Hoover, Natasha L.; Soupir, Michelle L.] Iowa State Univ, Dept Agr & Biosyst Engn, 1201 Sukup Hall, Ames, IA 50011 USA. [Bhandari, Alok] Kansas State Univ, Dept Civil Engn, Manhattan, KS 66506 USA. [Moorman, Thomas B.] USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. RP Soupir, ML (reprint author), Iowa State Univ, Dept Agr & Biosyst Engn, 1201 Sukup Hall, Ames, IA 50011 USA. EM msoupir@iastate.edu FU Iowa State University; Graduate Program for Sustainable Agriculture FX This work is dedicated to the memory of our colleague, mentor, and friend Alok Bhandari. This was his final research to be completed before his untimely passing. The authors would like to thank Loren Shires for assistance with nutrient analysis and Manu Ajmani and the DOE Academies Creating Teacher Scientists Program for assistance with sample collection. Funding for this work was provided by Iowa State University and the Graduate Program for Sustainable Agriculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 54 TC 3 Z9 3 U1 16 U2 25 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 803 EP 812 DI 10.2134/jeq2015.03.0161 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700007 PM 27136145 ER PT J AU Halvorson, AD Del Grosso, SJ Stewart, CE AF Halvorson, Ardell D. Del Grosso, Stephen J. Stewart, Catherine E. TI Manure and Inorganic Nitrogen Affect Trace Gas Emissions under Semi-Arid Irrigated Corn SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NO-TILL CORN; ENHANCED EFFICIENCY FERTILIZERS; OXIDE EMISSIONS; CARBON-DIOXIDE; CROPPING SYSTEMS; AMMONIA VOLATILIZATION; N2O EMISSIONS; DAIRY MANURE; SOIL; FLUXES AB Dairy manure is often applied to cropped soils as a substitute for inorganic N fertilizers, but the impacts of manure on soil trace gas fluxes, yields, and soil N are uncertain in the semiarid western United States. Soil carbon dioxide (CO2-C), methane (CH4-C), nitrous oxide (N2O-N), and ammonia (NH3-N) emissions were monitored using surface chambers from five N treatments: (i) partially composted solid dairy manure (DM) (412 kg N ha(-1)), (ii) DM + AgrotainPlus (DM+AP), (iii) enhanced efficiency N fertilizer (SuperU [SU]) (179 kg N ha(-1)), (iv) urea (179 kg N ha(-1)), and (v) check (no N applied), to determine their effect on growing season (GS) and nongrowing season emissions from a tilled clay loam soil under irrigated, continuous corn production for 3 yr. SuperU and AgrotainPlus contain urease and nitrification inhibitors. Averaged over years, GS soil CO2-C emissions were greater for DM and DM+AP than for urea, SU, and check treatments due to the large amount of C added with the manure; CH4-C emissions did not vary among N treatments; and N2O-N emissions decreased in the order urea = DM = DM+AP > SU > check. AgrotainPlus added to the DM did not reduce N2O-N emissions compared with DM. Cumulative NH3-N emissions after manure application decreased in the order urea > SU > check, with no significant differences between SU, DM, and DM+AP. Dairy manure provided slow-release N with nitrate intensities lower than urea and N2O-N emissions similar to urea. These results highlight the importance of best-management practices such as immediate irrigation after N application and use of urease and nitrification inhibitors to minimize N losses. C1 [Halvorson, Ardell D.; Del Grosso, Stephen J.; Stewart, Catherine E.] USDA ARS, 2150 Ctr Ave,Bldg D,Ste 100, Ft Collins, CO 80526 USA. RP Del Grosso, SJ (reprint author), USDA ARS, 2150 Ctr Ave,Bldg D,Ste 100, Ft Collins, CO 80526 USA. EM Steve.DelGrosso@ars.usda.gov OI Stewart, Catherine/0000-0003-1216-0450 FU Agricultural Research Service under the ARS GRACEnet Project FX The authors thank B. Floyd, R. D'Adamo, A. Brandt, T. Delorean, M. Smith, M. Reyes-Fox, and students for their assistance and analytical support in collecting, processing, and analyzing the soil, gas, and plant samples and data reported herein; the ARDEC Staff for help with plot maintenance; and Koch Agronomic Services (Wichita, KS) for providing the SuperU and AgrotainPlus used in the study. This publication is based on work supported by the Agricultural Research Service under the ARS GRACEnet Project. NR 53 TC 1 Z9 1 U1 4 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 906 EP 914 DI 10.2134/jeq2015.08.0426 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700019 PM 27136157 ER PT J AU Trabue, S Kerr, B Scoggin, K AF Trabue, Steven Kerr, Brian Scoggin, Kenwood TI Odor and Odorous Compound Emissions from Manure of Swine Fed Standard and Dried Distillers Grains with Soluble Supplemented Diets SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID REDUCED CRUDE PROTEIN; HYDROGEN-SULFIDE EMISSIONS; VOLATILE ORGANIC-COMPOUNDS; REACTION MASS-SPECTROMETRY; ANIMAL FEEDING OPERATIONS; GROWING-FINISHING PIGS; FIELD SAMPLING METHOD; AMMONIA EMISSIONS; NUTRIENT DIGESTIBILITY; GROWTH-PERFORMANCE AB This study was conducted to determine the impact of diets containing dried distillers grains with solubles (DDGS) on emissions of odor and odorous compounds from swine manure storage. Twenty-four pigs were fed either a corn-soybean meal (CSBM) diet or a diet containing 35% DDGS over a 42-d feeding trial. Their waste was collected and transferred to individual manure storage containers. Manure from pigs fed diets containing DDGS had significantly lower odorant emissions expressed in animal units for hydrogen sulfide (H2S) and ammonia (NH3) (P < 0.05) compared with pigs fed the CSBM diet, but emissions of volatile fatty acids and phenolic compounds were significantly higher (P < 0.05) for manures from animals fed the DDGS diet. There was no significant difference for indole compound emissions due to the dietary treatment applied. Emissions of volatile organic compounds (VOCs) from manure accounted for less than 0.1% of carbon consumed for either diet. There were no significant differences in odor emissions for either diet as quantified with human panels or measured as the sum total of the odor activity value. Manure odors from pigs fed the CSBM diet were dominated by H2S, whereas animals fed the diet containing DDGS were dominated by VOCs. C1 [Trabue, Steven; Kerr, Brian; Scoggin, Kenwood] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Trabue, S (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM steven.trabue@ars.usda.gov NR 91 TC 1 Z9 1 U1 6 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 915 EP 923 DI 10.2134/jeq2015.10.0511 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700020 PM 27136158 ER PT J AU Park, Y Pachepsky, Y Shelton, D Jeong, J Whelan, G AF Park, Yongeun Pachepsky, Yakov Shelton, Daniel Jeong, Jaehak Whelan, Gene TI Survival of Manure-borne Escherichia coli and Fecal Coliforms in Soil: Temperature Dependence as Affected by Site-Specific Factors SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID AMENDED SOIL; LABORATORY CONDITIONS; INDICATOR ORGANISMS; PATHOGEN SURVIVAL; SEWAGE-SLUDGE; E. COLI; DIE-OFF; FATE; WATER; TRANSPORT AB Understanding pathogenic and indicator bacteria survival in soils is essential for assessing the potential of microbial contamination of water and produce. The objective of this work was to evaluate the effects of soil properties, animal source, experimental conditions, and the application method on temperature dependencies of manure-borne generic Escherichia coli, E. coli O157: H7, and fecal coliforms survival in soils. A literature search yielded 151 survival datasets from 70 publications. Either one-stage or two-stage kinetics was observed in the survival datasets. We used duration and rate of the logarithm of concentration change as parameters of the first stage in the two-stage kinetics data. The second stage of the two-stage kinetics and the one-stage kinetics were simulated with the Q(10) model to find the dependence of the inactivation rate on temperature. Classification and regression trees and linear regressions were applied to parameterize the kinetics. Presence or absence of two-stage kinetics was controlled by temperature, soil texture, soil water content, and for fine-textured soils by setting experiments in the field or in the laboratory. The duration of the first stage was predominantly affected by soil water content and temperature. In the Q(10) model dependencies of inactivation rates on temperature, parameter Q(10) estimates were significantly affected by the laboratory versus field conditions and by the application method, whereas inactivation rates at 20 degrees C were significantly affected by all survival and management factors. Results of this work can provide estimates of coliform survival parameters for models of microbial water quality. C1 [Park, Yongeun; Pachepsky, Yakov; Shelton, Daniel] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Jeong, Jaehak] Texas A&M Univ, Dept Biol & Agr Engn, Temple, TX 76504 USA. [Whelan, Gene] US EPA, Natl Exposure Res Lab, Ecosyst Res Div, Athens, GA 30605 USA. RP Pachepsky, Y (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Park, Yongeun/0000-0002-1959-0843 FU USEPA through its Office of Research and Development [DW-12-92348101] FX The authors thank Gonzalo Martinez and Matthew Stocker for help with data collection. The USEPA through its Office of Research and Development partially funded and collaborated in the research described here under DW-12-92348101 to the USDA-ARS. It has been subjected to agency review and approved for publication. NR 68 TC 3 Z9 3 U1 9 U2 18 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 949 EP 957 DI 10.2134/jeq2015.08.0427 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700024 PM 27136162 ER PT J AU Brooks, JP McLaughlin, MR Adeli, A Miles, DM AF Brooks, J. P. McLaughlin, M. R. Adeli, A. Miles, D. M. TI Cultivation and qPCR Detection of Pathogenic and Antibiotic-Resistant Bacterial Establishment in Naive Broiler Houses SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID REAL-TIME PCR; NATURAL ENVIRONMENTS; CAMPYLOBACTER SPP.; POULTRY LITTER; UNITED-STATES; WASTE-WATER; SALMONELLA; GENES; MANURE; ASSAY AB Conventional commercial broiler production involves the rearing of more than 20,000 broilers in a single confined space for approximately 6.5 wk. This environment is known for harboring pathogens and antibiotic-resistant bacteria, but studies have focused on previously established houses with mature litter microbial populations. In the current study, a set of three naive houses were followed from inception through 11 broiler flocks and monitored for ambient climatic conditions, bacterial pathogens, and antibiotic resistance. Within the first 3 wk of the first flock cycle, 100% of litter samples were positive for Salmonella and Listeria, whereas Campylobacter was cultivation negative but PCR positive. Antibiotic resistance genes were ubiquitously distributed throughout the litter within the first flock, approaching 107 to 1012 genomic units g(-1). Preflock litter levels were approximately 106 CFU g(-1) for heterotrophic plate count bacteria, whereas midflock levels were >1011 colony forming units (CFU) g(-1); other indicators demonstrated similar increases. The influence of intrahouse sample location was minor. In all likelihood, given that preflock levels were negative for pathogens and antibiotic resistance genes and 4 to 5 Log(10) lower than flock levels for indicators, incoming birds most likely provided the colonizing microbiome, although other sources were not ruled out. Most bacterial groups experienced a cyclical pattern of litter contamination seen in other studies, whereas microbial stabilization required approximately four flocks. This study represents a first-of-its-kind view into the time required for bacterial pathogens and antibiotic resistance to colonize and establish in naive broiler houses. C1 [Brooks, J. P.; McLaughlin, M. R.; Adeli, A.; Miles, D. M.] USDA ARS, Genet & Sustainable Agr Unit, Mississippi State, MS 39762 USA. RP Brooks, JP (reprint author), USDA ARS, Genet & Sustainable Agr Unit, Mississippi State, MS 39762 USA. EM john.brooks@ars.usda.gov NR 33 TC 1 Z9 1 U1 3 U2 13 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 958 EP 966 DI 10.2134/jeq2015.09.0492 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700025 PM 27136163 ER PT J AU Phillips, CL Trippe, KM Whittaker, G Griffith, SM Johnson, MG Banowetz, GM AF Phillips, Claire L. Trippe, Kristin M. Whittaker, Gerald Griffith, Stephen M. Johnson, Mark G. Banowetz, Gary M. TI Gasified Grass and Wood Biochars Facilitate Plant Establishment in Acid Mine Soils SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID METAL RETENTION; HEAVY-METALS; REMEDIATION; AMENDMENTS; IMMOBILIZATION; GASIFICATION; FEEDSTOCKS; MECHANISMS; DRAINAGE; INCREASE AB Heavy metals in exposed mine tailings threaten ecosystems that surround thousands of abandoned mines in the United States. Biochars derived from the pyrolysis or gasification of biomass may serve as a valuable soil amendment to revegetate mine sites. We evaluated the ability of two biochars, produced by gasification of either Kentucky bluegrass seed screenings (KB) or mixed conifer wood (CW), to support the growth of plants in mine spoils from the abandoned Formosa and Almeda Mines in Oregon. To evaluate the potential for plant establishment in mine tailings, wheat was grown in tailings amended with biochar at rates ranging from 0 to 9% (w/w). Both KB and CW biochars promoted plant establishment by increasing soil pH, increasing concentrations of macro-and micronutrients, and decreasing the solubility and plant uptake of heavy metals. Formosa tailings required at least 4% biochar and Almeda soil required at least 2% biochar to promote healthy wheat growth. A complimentary experiment in which mine spoils were leached with simulated precipitation indicated that biochar amendment rates >= 4% were sufficient to neutralize the elution pH and reduce concentrations of potentially toxic elements (Zn, Cu, Ni, Al) to levels near or below concern. These findings support the use of gasified biochar amendments to revegetate acid mine soils. C1 [Phillips, Claire L.; Trippe, Kristin M.; Whittaker, Gerald; Griffith, Stephen M.; Banowetz, Gary M.] USDA ARS, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. [Johnson, Mark G.] US EPA, Natl Hlth & Environm Effects Res Lab, 200 SW 35th St, Corvallis, OR 97333 USA. RP Trippe, KM (reprint author), USDA ARS, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM Kristin.Trippe@ars.usda.gov FU USDA-ARS [2072-21410-004-00D]; USEPA FX Research in the laboratory of K.T. and J.W. is supported by USDA-ARS Project #2072-21410-004-00D. The information in this document has been funded in part by the USEPA. It has been subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the USDA, the ARS, or the USEPA of any product or service to the exclusion of others that may be suitable. NR 45 TC 1 Z9 1 U1 7 U2 11 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 1013 EP 1020 DI 10.2134/jeq2015.09.0470 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700031 PM 27136169 ER PT J AU Kibet, LC Bryant, RB Buda, AR Kleinman, PJA Saporito, LS Allen, AL Hashem, FM May, EB AF Kibet, Leonard C. Bryant, Ray B. Buda, Anthony R. Kleinman, Peter J. A. Saporito, Louis S. Allen, Arthur L. Hashem, Fawzy M. May, Eric B. TI Persistence and Surface Transport of Urea-Nitrogen: A Rainfall Simulation Study SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID HARMFUL ALGAL BLOOMS; NITZSCHIA-AUSTRALIS BACILLARIOPHYCEAE; PSEUDO-NITZSCHIA; UNITED-STATES; DOMOIC ACID; SUBSURFACE APPLICATION; PHOSPHORUS TRANSFERS; LARGE WATERSHEDS; CHESAPEAKE BAY; POULTRY LITTER AB Studies of harmful algal blooms and associated urea concentrations in the Chesapeake Bay and in coastal areas around the globe strongly suggest that elevated urea concentrations are associated with harmful algal blooms. The observed increased frequency and toxicity of these blooms in recent decades has been correlated with increased agricultural use of N inputs and increased use of urea as a preferred form of commercial N. This rainfall simulation study sought to assess the potential for different N fertilizers and manures to contribute to urea in runoff from a Coastal Plain soil on the Eastern Shore of Maryland. Under worst-case conditions, similar to 1% of urea-N applied as commercial fertilizer and surface-applied poultry litter was lost in runoff in a simulated rainfall event, roughly equivalent to a 1-yr return period rain storm in the study area, 12 h after application. Cumulative urea-N losses, including four subsequent weekly rainfall events, approached 1.7% from urea-N fertilizer containing a urease inhibitor. Urea-N loss from incorporated poultry litter was negligible, and losses from dairy manure were intermediate. These losses are likely confined to hydrological contributing areas that extend several meters from a drainage ditch or stream for storms with frequent recurrence intervals. Cumulative dissolved N losses in runoff (urea-N + ammonium-N + nitrate-N) as a proportion of total applied plant-available N were <5%, suggesting that most of the applied N was lost by other pathways or was immobilized in soil. Results also highlight the potential for simple management options, such as shallow incorporation or timing, to greatly reduce urea runoff losses. C1 [Kibet, Leonard C.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68588 USA. [Bryant, Ray B.; Buda, Anthony R.; Kleinman, Peter J. A.; Saporito, Louis S.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Allen, Arthur L.; Hashem, Fawzy M.; May, Eric B.] Univ Maryland Eastern Shore, Princess Anne, MD 21853 USA. RP Bryant, RB (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM ray.bryant@ars.usda.gov FU USDA National Institute of Food and Agriculture Capacity Building Grant; USDA Agricultural Research Service FX The authors thank the staff and students of the University of Maryland Eastern Shore's Nutrient Management Laboratory for their valuable contributions to this study. Don Mahan assisted with assembly and operation of the rainfall simulator. Janice Donohoe led and supervised a dedicated group of undergraduate and graduate students who processed and analyzed samples. This project was funded by a USDA National Institute of Food and Agriculture Capacity Building Grant and the USDA Agricultural Research Service. NR 56 TC 0 Z9 0 U1 7 U2 14 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 1062 EP 1070 DI 10.2134/jeq2015.09.0495 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700037 PM 27136175 ER PT J AU Watts, DB Torbert, HA AF Watts, Dexter B. Torbert, H. Allen TI Influence of Flue Gas Desulfurization Gypsum on Reducing Soluble Phosphorus in Successive Runoff Events from a Coastal Plain Bermudagrass Pasture SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID EXTRACTABLE SOIL-PHOSPHORUS; COMBUSTION BY-PRODUCTS; MANURE PHOSPHORUS; SURFACE WATERS; POULTRY LITTER; NITROGEN; LOSSES; AMENDMENTS; IMPACT; SOLUBILITY AB Controlling the threat that pastures intensively managed with poultry litter (PL) pose to accelerating eutrophication is a major issue in the southeastern United States. Gypsum (CaSO4) has been identified as a promising management tool for ameliorating litter P losses to runoff. Thus, research was conducted to elucidate gypsum's residual effects on P losses from a bermudagrass (Cynodon dactylon L.) pasture. Runoff events (60 min) were created using rainfall simulations. Treatments consisted of applying four flue gas desulfurization (FGD) gypsum rates (0, 2.2, 4.4, and 8.9 Mg ha(-1)) to bermudagrass fertilized with 13.4 Mg ha(-1) PL plus a nonfertilized check (no litter or gypsum) and 8.9 Mg ha(-1) FGD gypsum only as controls. Rainfall simulations (similar to 85 mm h(-1)) were conducted immediately, 5 wk, and 6 mo (i.e., at the end of growing season) after PL application to determine gypsum's effectiveness at controlling P loss over successive runoff events. The greatest dissolved P (DP) in runoff occurred immediately after PL application. Gypsum effectively reduced cumulative DP concentration losses (54%) compared with PL alone in initial runoff events. Gypsum reduced DP concentrations in succeeding runoff events also regardless of timing, suggesting that its effect is persistent and will not diminish over a growing season. Generally, maximum DP reductions were achieved with 8.9 Mg ha-1. However, it was surmised from this study that optimal P reduction in a bermudagrass pasture can be achieved with 4.4 Mg ha-1. Information ascertained from this study may be useful in aiding land managers making prescriptions for management practices that reduce DP losses from agricultural fields. C1 [Watts, Dexter B.; Torbert, H. Allen] USDA ARS, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. RP Watts, DB (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 S Donahue Dr, Auburn, AL 36832 USA. EM Dexter.Watts@ars.usda.gov FU Southern Company; Tennessee Valley Authority; USEPA; USDA-ARS FX The authors thank the E.V. Smith Agriculture Research and Extension Center, Field Crops Unit for help in managing the field operations and James Ashley Robinson and Morris G. Welch for technical assistance. This research was funded in part by Southern Company, Tennessee Valley Authority, Duke Energy, USEPA, and USDA-ARS appropriated funds. NR 39 TC 3 Z9 3 U1 0 U2 3 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD MAY-JUN PY 2016 VL 45 IS 3 BP 1071 EP 1079 DI 10.2134/jeq2015.04.0203 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK8EJ UT WOS:000375159700038 PM 27136176 ER PT J AU Gamble, GR Berrang, ME Buhr, RJ Hinton, A Bourassa, DV Johnston, JJ Ingram, KD Adams, ES Feldner, PW AF Gamble, Gary R. Berrang, Mark E. Buhr, R. Jeff Hinton, Arthur, Jr. Bourassa, Dianna V. Johnston, John J. Ingram, Kimberly D. Adams, Eric S. Feldner, Peggy W. TI Effect of Simulated Sanitizer Carryover on Recovery of Salmonella from Broiler Carcass Rinsates SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Broiler carcass; Buffered peptone; Carryover; Salmonella; Sanitizer ID ANTIMICROBIAL TREATMENT; REDUCTION; GROWTH; PH AB Numerous antimicrobial chemicals are currently utilized as processing aids with the aim of reducing pathogenic bacteria on processed poultry carcasses. Carryover of active sanitizer to a carcass rinse solution intended for recovery of viable pathogenic bacteria by regulatory agencies may cause false-negative results. This study was conducted to document the potential carryover effect of five sanitizing chemicals commonly used as poultry processing aids for broilers in a postchill dip. The effect of postdip drip time on the volume of sanitizer solution carryover was first determined by regression of data obtained from 10 carcasses. The five sanitizer solutions were diluted with buffered peptone water at 0-, 1-, and 5-min drip time equivalent volumes as determined by the regression analysis. These solutions were then spiked to 10(5) CFU/ml with a mixture of five nalidixic acid-resistant Salmonella enterica serovars, stored at 4 degrees C for 24 h, and finally enumerated by plate count on brilliant green sulfa agar containing nalidixic acid. At the 0- and 1-min drip time equivalents, no Salmonella recovery was observed in three of the five sanitizers studied. At the 5-min drip time equivalent, one of these sanitizers still exhibited significant (P < 0.05) bactericidal activity. These findings potentially indicate that the currently utilized protocol for the recovery of Salmonella bacteria from postchill sanitizer interventions may lead to false-negative results due to sanitizer carryover into the carcass rinsate. C1 [Gamble, Gary R.; Berrang, Mark E.; Buhr, R. Jeff; Hinton, Arthur, Jr.; Bourassa, Dianna V.; Ingram, Kimberly D.; Adams, Eric S.; Feldner, Peggy W.] US Natl Poultry Res Ctr, ARS, USDA, Athens, GA 30605 USA. [Johnston, John J.] Food Safety & Inspect Serv, USDA, Off Publ Hlth Sci, Bldg D,Suite 320, Ft Collins, CO 80526 USA. RP Gamble, GR (reprint author), US Natl Poultry Res Ctr, ARS, USDA, Athens, GA 30605 USA. EM gary.gamble@ars.usda.gov NR 17 TC 1 Z9 1 U1 0 U2 0 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2016 VL 79 IS 5 BP 710 EP 714 DI 10.4315/0362-028X.JFP-15-461 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DL2UM UT WOS:000375490700003 PM 27296416 ER PT J AU Porto-Fett, ACS Oliver, M Daniel, M Shoyer, BA Stahler, LJ Shane, LE Kassama, LS Jackson-Davis, A Luchansky, JB AF Porto-Fett, Anna C. S. Oliver, Michelle Daniel, Marciauna Shoyer, Bradley A. Stahler, Laura J. Shane, Laura E. Kassama, Lamin S. Jackson-Davis, Armitra Luchansky, John B. TI Effect of Deep-Frying or Conventional Oven Cooking on Thermal Inactivation of Shiga Toxin-Producing Cells of Escherichia coli in Meatballs SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Cooking; Ground meat; Meatballs; Shiga-toxin producing Escherichia coli; Thermal inactivation; Veal ID GROUND-BEEF PATTIES; OPEN-FLAME GAS; O157H7; O157-H7; STEAKS; SALMONELLA; SURVIVAL; O157/H7; VEAL; APPLIANCES AB We investigated the effects of deep-frying or oven cooking on inactivation of Shiga toxin producing cells of Escherichia coli (STEC) in meatballs. Finely ground veal and/or a finely ground beef-pork-veal mixture were inoculated (ca. 6.5 log CFU/g) with an eight-strain, genetically marked cocktail of rifampin-resistant STEC strains (STEC-8; O111:H, O45:H2, O103:H2, O104:H4, O121:H19, O145:NM, O26:H11, and O157:H7). Inoculated meat was mixed with liquid whole eggs and seasoned bread crumbs, shaped by hand into 40-g balls, and stored at -20 degrees C (i.e., frozen) or at 4 degrees C (i.e., fresh) for up to 18 h. Meatballs were deep-fried (canola oil) or baked (convection oven) for up to 9 or 20 min at 176.7 degrees C (350 F), respectively. Cooked and uncooked samples were homogenized and plated onto sorbitol MacConkey agar with rifampin (100 g/ml) followed by incubation of plates at 37 degrees C for ca. 24 h. Up to four trials and three replications for each treatment for each trial were conducted. Deep-frying fresh meatballs for up to 5.5 min or frozen meatballs for up to 9.0 min resulted in reductions of STEC-8 ranging from ca. 0.7 to >= 6.1 log CFU/g. Likewise, reductions of ca. 0.7 to >= 6.1 log CFU/g were observed for frozen and fresh meatballs that were oven cooked for 7.5 to 20 min. This work provides new information on the effect of prior storage temperature (refrigerated or frozen), as well as subsequent cooking via deep-frying or baking, on inactivation of S in meatballs prepared with beef, pork, and/or veal. These results will help establish guidelines and best practices for cooking raw meatballs at both food service establishments and in the home. C1 [Porto-Fett, Anna C. S.; Shoyer, Bradley A.; Stahler, Laura J.; Shane, Laura E.; Luchansky, John B.] ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Oliver, Michelle; Daniel, Marciauna; Kassama, Lamin S.; Jackson-Davis, Armitra] Alabama A&M Univ, Dept Food & Anim Sci, Normal, AL 35762 USA. RP Porto-Fett, ACS (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM anna.portofett@ars.usda.gov FU National Institute of Food and Agriculture, USDA [2012-68003-30155] FX We extend our sincere appreciation to Manuela Osoria (USDA, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA) for her assistance on this project. We extend a special note of thanks to Dr. Bryan Vinyard (USDA, ARS, Beltsville Agricultural Research Center, Beltsville, MD) for statistically analyzing these data. This material is based upon work supported by the National Institute of Food and Agriculture, USDA, under award no. 2012-68003-30155. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 49 TC 0 Z9 0 U1 5 U2 9 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD MAY PY 2016 VL 79 IS 5 BP 723 EP 731 DI 10.4315/0362-028X.JFP-15-427 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DL2UM UT WOS:000375490700005 PM 27296418 ER PT J AU Gould, GK Liu, ML Barber, ME Cherkauer, KA Robichaud, PR Adam, JC AF Gould, Gregory K. Liu, Mingliang Barber, Michael E. Cherkauer, Keith A. Robichaud, Peter R. Adam, Jennifer C. TI The effects of climate change and extreme wildfire events on runoff erosion over a mountain watershed SO JOURNAL OF HYDROLOGY LA English DT Article DE Wildfire; Erosion; Climate change; WEPP model; Hydrology ID CONTERMINOUS UNITED-STATES; DAILY SOLAR-RADIATION; COLUMBIA RIVER-BASIN; COLORADO FRONT RANGE; PACIFIC-NORTHWEST; POSTFIRE RUNOFF; HYDROLOGICAL RESPONSE; SEDIMENT DELIVERY; CHANGE SCENARIOS; WESTERN USA AB Increases in wildfire occurrence and severity under an altered climate can substantially impact terrestrial ecosystems through enhancing runoff erosion. Improved prediction tools that provide high resolution spatial information are necessary for location-specific soil conservation and watershed management. However, quantifying the magnitude of soil erosion and its interactions with climate, hydrological processes, and fire occurrences across a large region (>10,000 km(2)) is challenging because of the large computational requirements needed to capture the fine-scale complexities of the land surface that govern erosion. We apply the physically-based coupled Variable Capacity Infiltration-Water Erosion Prediction Project (VIC-WEPP) model to study how wildfire occurrences can enhance soil erosion in a future climate over a representative watershed in the northern Rocky Mountains - the Salmon River Basin (SRB) in central Idaho. While the VIC model simulates hydrologic processes at larger scales, the WEPP model simulates erosion at the hillslope scale by sampling representative hillslopes. VIC-WEPP model results indicate that SRB streamflow will have an earlier shift in peak flow by one to two months under future climate scenarios in response to a declining snowpack under warming temperatures. The magnitude of peak flow increases with each higher severity fire scenario; and under the highest fire severity, the peak flow is shifted even earlier, exacerbating the effects of climate change. Similarly, sediment yield also increases with higher fire severities for both historical and future climates. Sediment yield is more sensitive to fire occurrence than to climate change by one to two orders of magnitude, which is not unexpected given that our fire scenarios were applied basin wide as worst case scenarios. In reality, fires only occur over portions of the basin in any given year and subsequent years' vegetation regrowth reduces erosion. However, the effects of climate change on sediment yield result in greater spatial heterogeneities, primarily because of the spatial differences in precipitation projections, while fire conditions were uniformly applied. The combined effects of climate change and a possible continuation of increasing fire frequency and severity will compound excess sediment issues that already exist in this region of the intermountain West. (C) 2016 Elsevier B.V. All rights reserved. C1 [Gould, Gregory K.; Liu, Mingliang; Barber, Michael E.; Adam, Jennifer C.] Washington State Univ, Dept Civil & Environm Engn, Pullman, WA 99163 USA. [Cherkauer, Keith A.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Robichaud, Peter R.] US Forest Serv, Rocky Mt Res Stn, USDA, Moscow, ID 83843 USA. [Barber, Michael E.] Univ Utah, Dept Civil & Environm Engn, Salt Lake City, UT 84112 USA. RP Adam, JC (reprint author), Washington State Univ, Dept Civil & Environm Engn, Pullman, WA 99163 USA. EM jcadam@wsu.edu RI Liu, Mingliang/B-1361-2009 FU Department of Agriculture, National Institute of Food and Agriculture through the Water Sustainability and Climate program [2012-67003-19805]; State of Washington Water Research Center through the United States Geological Survey 104(b) funding [G11AP20113] FX This research is funded by the Department of Agriculture, National Institute of Food and Agriculture Grant Number 2012-67003-19805 through the Water Sustainability and Climate program, and by the State of Washington Water Research Center Grant Number G11AP20113 through the United States Geological Survey 104(b) funding to the Water Resource Research Institutes. The authors would also like to thank Alan Hamlet for insights related to calibration of the VIC model over the Salmon River basin, Lili Wang for support in implementation of VIC-WEPP, and Joe Wagenbrenner for help in identifying parameters for WEPP in post-fire environments. Finally, we would like to thank two anonymous reviewers for their thoughtful comments that improved the manuscript. NR 112 TC 0 Z9 0 U1 33 U2 54 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD MAY PY 2016 VL 536 BP 74 EP 91 DI 10.1016/j.jhydrol.2016.02.025 PG 18 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DK3IV UT WOS:000374811200007 ER PT J AU Wang, H Khera, P Huang, BY Yuan, M Katam, R Zhuang, WJ Harris-Shultz, K Moore, KM Culbreath, AK Zhang, XY Varshney, RK Xie, LH Guo, BZ AF Wang, Hui Khera, Pawan Huang, Bingyan Yuan, Mei Katam, Ramesh Zhuang, Weijian Harris-Shultz, Karen Moore, Kim M. Culbreath, Albert K. Zhang, Xinyou Varshney, Rajeev K. Xie, Lianhui Guo, Baozhu TI Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Article DE Arachis hypogaea; breeding; genetic diversity; population structure; simple sequence repeat ID ARACHIS-HYPOGAEA L.; MINI-CORE COLLECTION; SSR MARKERS; MICROSATELLITE MARKERS; GERMPLASM COLLECTION; ASSOCIATION ANALYSIS; LINKAGE MAPS; RAPD MARKERS; OIL QUALITY; GROUNDNUT AB Cultivated peanut is grown worldwide as rich-source of oil and protein. A broad genetic base is needed for cultivar improvement. The objectives of this study were to develop highly informative simple sequence repeat (SSR) markers and to assess the genetic diversity and population structure of peanut cultivars and breeding lines from different breeding programs in China, India and the US. A total of 111 SSR markers were selected for this study, resulting in a total of 472 alleles. The mean values of gene diversity and polymorphic information content (PIC) were 0.480 and 0.429, respectively. Country-wise analysis revealed that alleles per locus in three countries were similar. The mean gene diversity in the US, China and India was 0.363, 0.489 and 0.47 with an average PIC of 0.323, 0.43 and 0.412, respectively. Genetic analysis using the STRUCTURE divided these peanut lines into two populations (P1, P2), which was consistent with the dendrogram based on genetic distance (G1, G2) and the clustering of principal component analysis. The groupings were related to peanut market types and the geographic origin with a few admixtures. The results could be used by breeding programs to assess the genetic diversity of breeding materials to broaden the genetic base and for molecular genetics studies. C1 [Wang, Hui; Xie, Lianhui] Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350002, Peoples R China. [Wang, Hui; Khera, Pawan; Culbreath, Albert K.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31794 USA. [Wang, Hui; Guo, Baozhu] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31794 USA. [Wang, Hui; Yuan, Mei] Shandong Peanut Res Inst, Qingdao 266100, Peoples R China. [Khera, Pawan; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. [Huang, Bingyan; Zhang, Xinyou] Henan Acad Agr Sci, Cash Crops Res Inst, Zhengzhou 450002, Peoples R China. [Katam, Ramesh] Florida A&M Univ, Dept Biol Sci, Tallahassee, FL 32307 USA. [Zhuang, Weijian] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Peoples R China. [Harris-Shultz, Karen] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31794 USA. [Moore, Kim M.] AgRes Consultants, Shingler Little River Rd, Sumner, GA 31789 USA. RP Xie, LH (reprint author), Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350002, Peoples R China.; Guo, BZ (reprint author), USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31794 USA. EM fjxlh@126.com; baozhu.guo@ars.usda.gov FU US Department of Agriculture Agricultural Research Service (USDA-ARS); Georgia Agricultural Commodity Commission for Peanuts; Peanut Foundation; National Peanut Board FX We thank Liming Yang, Jake Fountain and Billy Wilson for technical assistance in the greenhouse and the laboratory. This work is partially supported by the US Department of Agriculture Agricultural Research Service (USDA-ARS), the Georgia Agricultural Commodity Commission for Peanuts, Peanut Foundation and National Peanut Board. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and USAID University Linkage Grant. ICRISAT is a member of CGIAR Consortium. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer. NR 63 TC 1 Z9 1 U1 10 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD MAY PY 2016 VL 58 IS 5 BP 452 EP 465 DI 10.1111/jipb.12380 PG 14 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DK7ZU UT WOS:000375146800002 PM 26178804 ER PT J AU Huang, TY Tobias, DK Hruby, A Rifai, N Tworoger, SS Hu, FB AF Huang, Tianyi Tobias, Deirdre K. Hruby, Adela Rifai, Nader Tworoger, Shelley S. Hu, Frank B. TI An Increase in Dietary Quality Is Associated with Favorable Plasma Biomarkers of the Brain-Adipose Axis in Apparently Healthy US Women SO JOURNAL OF NUTRITION LA English DT Article DE dietary quality; brain-adipose axis; leptin; soluble leptin receptor; adiponectin ID SOLUBLE LEPTIN RECEPTOR; BODY-FAT DISTRIBUTION; C-REACTIVE PROTEIN; CARDIOVASCULAR-DISEASE; INSULIN-RESISTANCE; WEIGHT-LOSS; ENDOTHELIAL DYSFUNCTION; INFLAMMATORY MARKERS; LOW-CARBOHYDRATE; LIFE-STYLE AB Background: The associations between long-term dietary quality and biomarkers of the brain-adipose axis have not been examined. Objective: We evaluated both cross-sectional and longitudinal associations between dietary quality and several biomarkers involved in the brain-adipose axis. Methods: In the Nurses' Health Study II, 831 women [baseline mean age: 45 y; body mass index (BMI; in kg/m(2)): 24.61 were randomly selected from women who provided 2 fasting blood samples in 1996-1999 and 2010-2011 to measure plasma concentrations of leptin, soluble leptin receptor (sOB-R), adiponectin, insulin, retinol binding protein-4 (RBP-4), interleukin-6 (IL-6), and C-reactive protein (CRP) Dietary quality was assessed by the Alternative Healthy Eating Index (AHEI-2010) with the use of semiquantitative food-frequency questionnaires administered in 1995 and 2011. We used linear mixed models to evaluate the cross-sectional associations between dietary quality and biomarker concentrations. We also examined change in dietary quality in relation to change in biomarker concentrations. Results: In cross-sectional analyses that compared the highest with the lowest quintile of AHEI-2010, we observed significantly lower leptin (P-trend < 0.0001), insulin (P-trend < 0.0001), and CRP (P-trend = 0.02) and significantly higher sOB-R (P-trend < 0.0001) and adiponectin (P-trend = 0.0003). These associations, except for CRP, remained significant after adjustment for BMI. In longitudinal analyses, women in the highest quintile of AHEI-2010 score change (most improvement) had a 13% increase in leptin, compared with a 42% increase (P-trend < 0.0001) in the lowest quintile (least improvement). The corresponding multivariable-adjusted percentage changes for other biomarkers were 4% compared with -1% for sOB-R (P-trend = 0.04), 14% compared with 6% for adiponectin (P-trend = 0.02), and -11% compared with 16% for CRP (P-trend = 0.02). Adjustment for interim weight change attenuated these associations. No associations were observed for RBP-4 or IL-6. Conclusion: Improvement in dietary quality was associated with favorable profiles of several biomarkers of the brain-adipose axis in women. C1 [Huang, Tianyi; Tworoger, Shelley S.; Hu, Frank B.] Brigham & Womens Hosp, Dept Med, Channing Div Network Med, 75 Francis St, Boston, MA 02115 USA. [Tobias, Deirdre K.] Brigham & Womens Hosp, Dept Med, Div Prevent Med, 75 Francis St, Boston, MA 02115 USA. [Tobias, Deirdre K.] Harvard Univ, Sch Med, Boston, MA USA. [Hruby, Adela] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Epidemiol Program, Boston, MA 02111 USA. [Rifai, Nader] Boston Childrens Hosp, Div Lab Med, Boston, MA USA. [Tworoger, Shelley S.; Hu, Frank B.] Harvard Univ, Dept Epidemiol, TH Chan Sch Publ Hlth, Boston, MA 02115 USA. [Hu, Frank B.] Harvard Univ, TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. RP Huang, TY (reprint author), Brigham & Womens Hosp, Dept Med, Channing Div Network Med, 75 Francis St, Boston, MA 02115 USA. EM tih541@mail.harvard.edu OI Huang, Tianyi/0000-0001-8420-9167 FU NIH [UM1 CA176726, R01 CA67262, HL60712, 5UM1CA17672] FX Supported by NIH grants UM1 CA176726, R01 CA67262, HL60712, and 5UM1CA17672 NR 45 TC 1 Z9 1 U1 1 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2016 VL 146 IS 5 BP 1101 EP 1108 DI 10.3945/jn.115.229666 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DL0VA UT WOS:000375349500023 PM 27075907 ER PT J AU Williams, AM Chantry, CJ Young, SL Achando, BS Allen, LH Arnold, BF Colford, JM Dentz, HN Hampel, D Kiprotich, MC Lin, A Null, CA Nyambane, GM Shahab-Ferdows, S Stewart, CP AF Williams, Anne M. Chantry, Caroline J. Young, Sera L. Achando, Beryl S. Allen, Lindsay H. Arnold, Benjamin F. Colford, John M., Jr. Dentz, Holly N. Hampel, Daniela Kiprotich, Marion C. Lin, Audrie Null, Clair A. Nyambane, Geoffrey M. Shahab-Ferdows, Setti Stewart, Christine P. TI Vitamin B-12 Concentrations in Breast Milk Are Low and Are Not Associated with Reported Household Hunger, Recent, Animal-Source Food, or Vitamin B-12 Intake in Women in Rural Kenya SO JOURNAL OF NUTRITION LA English DT Article DE animal-source foods; breast milk; lactation; vitamin B-12; Kenya; Africa; hunger; food security ID INSULIN-RESISTANCE; FOLATE-DEFICIENCY; MONTHS POSTPARTUM; GUATEMALAN WOMEN; HIGHLY PREVALENT; INCOME COUNTRIES; CHILDREN; PREGNANCY; INFANTS; LACTATION AB Background: Breast milk vitamin B-12 concentration may be inadequate in regions in which animal-source food consumption is low or infrequent. Vitamin B-12 deficiency causes megaloblastic anemia and impairs growth and development in children. Objective: We measured vitamin B-12 in breast milk and examined its associations with household hunger, recent animal-source food consumption, and vitamin B-12 intake. Methods: In a cross-sectional substudy nested within a cluster-randomized trial assessing water, sanitation, hygiene, and nutrition interventions in Kenya, we sampled 286 women 1-6 mo postpartum. Mothers hand-expressed breast milk 1 min into a feeding after 90 min observed nonbreastfeeding. The Household Hunger Scale was used to measure hunger, food intake in the previous week was measured with the use of a food-frequency questionnaire (FFQ), and vitamin B-12 intake was estimated by using 24-h dietary recall. An animal-source food score was based on 10 items from the FFQ (range: 0-70). Breast milk vitamin B-12 concentration was measured with the use of a solid-phase competitive chemiluminescent enzyme immunoassay and was modeled with linear regression. Generalized estimating equations were used to account for correlated observations at the cluster level. Results: Median (IQR) vitamin B-12 intake was 1.5 mu g/d (0.3, 9.7 mu g/d), and 60% of women consumed <2.4 mu g/d, the estimated average requirement during lactation. Median (IQR) breast milk vitamin B-12 concentration was 113 pmol/L (61, 199 pmol/L); 89% had concentrations <310 pmol/L, the estimated adequate concentration. Moderate or severe hunger prevalence was 27%; the animal-source food score ranged from 0 to 30 item-d/wk. Hunger and recent animal-source food and vitamin B-12 intake were not associated with breast milk vitamin B-12 concentrations. Maternal age was negatively associated with breast milk vitamin B-12 concentrations. Conclusion: Most lactating Kenyan women consumed less than the estimated average requirement of vitamin B-12 and had low breast milk vitamin B-12 concentrations. We recommend interventions that improve vitamin B-12 intake in lactating Kenyan women to foster maternal health and child development. C1 [Williams, Anne M.; Chantry, Caroline J.; Allen, Lindsay H.; Dentz, Holly N.; Hampel, Daniela; Stewart, Christine P.] Univ Calif Davis, Dept Nutr, Program Int & Community Nutr, Davis, CA 95616 USA. [Young, Sera L.] Cornell Univ, Program Int Nutr, Dept Populat Med & Diagnost Sci, Ithaca, NY USA. [Achando, Beryl S.; Dentz, Holly N.; Kiprotich, Marion C.; Null, Clair A.; Nyambane, Geoffrey M.] Innovat Poverty Act, Nairobi, Kenya. [Achando, Beryl S.; Dentz, Holly N.; Kiprotich, Marion C.; Null, Clair A.; Nyambane, Geoffrey M.] Innovat Poverty Act, New Haven, CT USA. [Allen, Lindsay H.; Hampel, Daniela; Shahab-Ferdows, Setti] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Arnold, Benjamin F.; Colford, John M., Jr.; Lin, Audrie] Univ Calif Berkeley, Sch Publ Hlth, Dept Epidemiol, Berkeley, CA 94720 USA. [Null, Clair A.] Math Policy Res, Washington, DC USA. RP Stewart, CP (reprint author), Univ Calif Davis, Dept Nutr, Program Int & Community Nutr, Davis, CA 95616 USA. EM cpstewart@ucdavis.edu FU Bill & Melinda Gates Foundation [OPPGD759]; Henry A Jastro Graduate Research Award at the University of California, Davis; National Institute of Mental Health [K01 MH098902]; USDA Agricultural Research Service intramural project [2032-51000-004-00] FX Based on research funded by a grant to the University of California, Berkeley, from the Bill & Melinda Gates Foundation (OPPGD759). Additional funding was provided by the Henry A Jastro Graduate Research Award at the University of California, Davis. SLY was supported by the National Institute of Mental Health (K01 MH098902). LHA and SSF were supported by USDA Agricultural Research Service intramural project no. 2032-51000-004-00. NR 59 TC 0 Z9 0 U1 0 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD MAY PY 2016 VL 146 IS 5 BP 1125 EP 1131 DI 10.3945/jn.115.228189 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DL0VA UT WOS:000375349500026 PM 27075905 ER PT J AU Rinehart, SW Folliard, JN Raimondi, MP AF Rinehart, Stefanie Winston Folliard, Jennifer Noll Raimondi, Mary Pat TI Building a Connection between Senior Hunger and Health Outcomes SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material ID MALNUTRITION; NUTRITION; SOCIETY; LENGTH; COSTS; STAY C1 [Rinehart, Stefanie Winston] US Dept HHS, Legislat & Policy, Chicago, IL USA. [Folliard, Jennifer Noll] USDA, Legislat & Policy, Chicago, IL USA. [Raimondi, Mary Pat] Acad Nutr & Dietet, Strateg Policy & Partnerships, Chicago, IL USA. RP Rinehart, SW (reprint author), US Dept HHS, Legislat & Policy, Chicago, IL USA. NR 21 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD MAY PY 2016 VL 116 IS 5 BP 759 EP 763 DI 10.1016/j.jand.2016.02.009 PG 4 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DK7ZH UT WOS:000375145400003 PM 27126148 ER PT J AU Siddique, A Latona, NP Taylor, MM Liu, CK AF Siddique, Aisha Latona, Nicholas P. Taylor, Maryann M. Liu, Cheng-Kung TI Preparation of Biobased Sponges from Un-tanned Hides SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID IMPROVED MECHANICAL-PROPERTIES; FIBROUS NETWORKS; COMPOSITE FILMS; CROSS-LINKING; POLY(LACTIC ACID); COLLAGEN SPONGES; IONIC LIQUID; LEATHER; FIBERS; REGENERATION AB One of our research endeavors to address ongoing challenges faced by the U.S. hide and leather industries is to develop innovative uses and novel biobased products from hides to improve prospective markets and to secure a viable future for hides and leather industries. We had previously investigated the production of nonwoven, green composites, and films from collagen fiber networks, which were extracted from un-tanned hides and from tannery solid wastes, such as splits or trimmings. Recently, we investigated the preparation of biobased sponges from un-tanned, specifically limed hides, which have potential commercial applications in medical care. We also investigated the effects of processing steps such as bating and alkaline treatments using sodium hydroxide (NaOH) on the morphology and viscoelasticity of resultant sponges from un-tanned hides. Results showed that the treatments of fiber networks have significant effects on the properties of resultant sponges. The dynamic mechanical analysis showed alkaline treatments yielded stiffer sponges than limed and bated samples. SDS-PAGE analysis showed the molecular weights of sponges were maintained in the range as those of commercial collagen samples. Lastly the resultant sponges are in the desirable apparent density range for a potential application as a medical sponge. C1 [Latona, Nicholas P.; Taylor, Maryann M.; Liu, Cheng-Kung] USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. [Siddique, Aisha] Quaid I Azam Univ, Islamabad 45320, Pakistan. RP Liu, CK (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM ChengKung.Liu@ars.usda.gov NR 40 TC 0 Z9 0 U1 3 U2 3 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD MAY PY 2016 VL 111 IS 5 BP 192 EP 199 PG 8 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA DL0VV UT WOS:000375351600004 ER PT J AU Brown, P Juarez, B AF Brown, Patricia Juarez, Bernadette TI Untitled SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Geneva, Switzerland. [Juarez, Bernadette] USDA, APHIS, AC, Bethesda, MD USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,HHS, Geneva, Switzerland. NR 2 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD MAY PY 2016 VL 45 IS 5 BP 167 EP 167 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA DL1CJ UT WOS:000375369400017 PM 27096184 ER PT J AU Petervary, N Allen, T Stokes, WS Banks, RE AF Petervary, Nicolette Allen, Tim Stokes, William S. Banks, Ron E. TI Availability of online educational content concerning topics of animal welfare SO LAB ANIMAL LA English DT Article AB Animal. welfare is an important area of study for professionals in fields of animal care and use, and many turn to self-learning resources to gain a better understanding of topics in this area. We assessed the state of these self-learning resources by evaluating open access, freely available resources on the internet with respect to their content and the reliability of their information. We categorized content using a modified list of the topics described in the American College of Animal Welfare's Role Delineation Document, and we identified subject areas that are underrepresented among freely available resources. We identified that the field needs more content describing practical information on subtopics of animal transportation, humane education and economic issues in animal welfare. We also suggest a targeted approach to improve and increase particular aspects of content that concerns the impacts of human, animal and environment interactions on animal welfare. We recommend that veterinary societies place more emphasis on welfare policies in their websites. Additionally, the field of animal welfare would benefit from more available and authoritative information on certain species and uses of animals that are presently underrepresented. C1 [Petervary, Nicolette; Stokes, William S.] USDA, Anim & Plant Hlth Inspect Serv, Anim Care, Raleigh, NC USA. [Allen, Tim] ARS, USDA, Natl Agr Lib, Anim Welf Informat Ctr, Beltsville, MD USA. [Banks, Ron E.] Duke Univ, Off Anim Welf Assurance, Durham, NC USA. RP Petervary, N (reprint author), USDA, Anim & Plant Hlth Inspect Serv, Anim Care, Raleigh, NC USA. EM Nicolette.petervary@aphis.usda.gov NR 11 TC 0 Z9 0 U1 4 U2 5 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 EI 1548-4475 J9 LAB ANIMAL JI Lab Anim. PD MAY PY 2016 VL 45 IS 5 BP 173 EP 179 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA DL1CJ UT WOS:000375369400019 PM 27096187 ER PT J AU Picklo, MJ Murphy, EJ AF Picklo, Matthew J. Murphy, Eric J. TI A High-Fat, High-Oleic Diet, But Not a High-Fat, Saturated Diet, Reduces Hepatic alpha-Linolenic Acid and Eicosapentaenoic Acid Content in Mice SO LIPIDS LA English DT Article; Proceedings Paper CT Northern Great Plains Lipid Conference CY MAY 31-JUN 01, 2015 CL Univ Manitoba, Winnipeg, CANADA HO Univ Manitoba DE Obesity; Polyunsaturated fatty acids; alpha-Linolenic acid; Monounsaturated fatty acid; Liver ID ADIPOSE-TISSUE; DIFFERENTIAL OXIDATION; KETOGENIC DIET; MYRISTIC ACID; FISH-OIL; RAT; METABOLISM; N-3; CONVERSION; OBESITY AB Considerable research has focused upon the role of linoleic acid (LNA; 18:2n-6) as a competitive inhibitor of alpha-linolenic (ALA; 18:3n-3) metabolism; however, little data exist as to the impact of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) on ALA metabolism. We tested the hypothesis that a high SFA diet, compared to a high MUFA (oleic acid 18:1n-9) diet, reduces ALA conversion to long chain n-3 fatty acids. Mice were fed for 12 weeks on three diets: (1) a control, 16 % fat energy diet consisting of similar levels of SFA and MUFA (2) a 50 % fat energy high MUFA energy diet (35 % MUFA and 7 % SFA) or (3) a 50 % fat energy, high SFA energy diet (34 % SFA, 8 % MUFA). ALA and LNA content remained constant. Analysis of hepatic lipids demonstrated a selective reduction (40 %) in ALA but not LNA and a 35 % reduction in eicosapentaenoic acid (EPA; 20:5n-3) in the high MUFA mice compared to the other groups. Lower content of ALA was reflected in the neutral lipid fraction, while smaller levels of phospholipid esterified EPA and docosapentaenoic acid (DPA; 22:5n-3) were evident. Docosahexaenoic acid (DHA; 22:6n-3) content was elevated by the high SFA diet. Expression of Fads1 (Delta 5 desaturase) and Fads2 (Delta 6 desaturase) was elevated by the high MUFA and reduced by the high SFA diet. These data indicate that a high MUFA diet, but not a high SFA diet, reduces ALA metabolism and point to selective hepatic disposition of ALA versus LNA. C1 [Picklo, Matthew J.] USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. [Picklo, Matthew J.] Univ N Dakota, Dept Chem, Grand Forks, ND 58201 USA. [Murphy, Eric J.] Univ N Dakota, Dept Basic Sci, Sch Med, Grand Forks, ND 58201 USA. RP Picklo, MJ (reprint author), USDA ARS, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA.; Picklo, MJ (reprint author), Univ N Dakota, Dept Chem, Grand Forks, ND 58201 USA. EM matthew.picklo@ars.usda.gov NR 50 TC 3 Z9 3 U1 2 U2 11 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD MAY PY 2016 VL 51 IS 5 BP 537 EP 547 DI 10.1007/s11745-015-4106-9 PG 11 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DL0NE UT WOS:000375328900004 PM 26694605 ER PT J AU Sundaram, S Bukowski, MR Lie, WR Picklo, MJ Yan, L AF Sundaram, Sneha Bukowski, Michael R. Lie, Wen-Rong Picklo, Matthew J. Yan, Lin TI High-Fat Diets Containing Different Amounts of n3 and n6 Polyunsaturated Fatty Acids Modulate Inflammatory Cytokine Production in Mice SO LIPIDS LA English DT Article; Proceedings Paper CT Northern Great Plains Lipid Conference CY MAY 31-JUN 01, 2015 CL Univ Manitoba, Winnipeg, CANADA HO Univ Manitoba DE Polyunsaturated fatty acids; Adiposity; Adipokines; Obesity; Mice ID MONOCYTE CHEMOATTRACTANT PROTEIN-1; PLASMINOGEN-ACTIVATOR INHIBITOR-1; REVERSES INSULIN-RESISTANCE; ADIPOSE-TISSUE INFLAMMATION; GENE-EXPRESSION; OBESE SUBJECTS; FISH-OIL; EICOSAPENTAENOIC ACID; HORMONE ADIPONECTIN; WEIGHT-LOSS AB Dysregulation of adipokines is a hallmark of obesity. Polyunsaturated fatty acids in fish oil may exert anti-inflammatory effects on adipose tissue mitigating the dysregulation of adipokines thereby preventing obesity. This study investigated the effects of high-fat diets containing different amounts of n3 polyunsaturated fatty acids (PUFA) on adiposity and adipokine production in mice. Mice were fed a low-fat or a high-fat diet with 16 or 45 % of energy from corn oil (low n3 PUFA) in comparison with a high-fat diet containing soybean or high-oleic sunflower oil (adequate n3 PUFA) or flaxseed or fish oil (high n3 PUFA) for 11 weeks. High-fat diets, regardless of types of oils, significantly increased body fat mass and body weights compared to the low-fat diet. Adipose fatty acid composition and contents reflected dietary fatty acid profiles. The high-fat fish oil diet significantly increased adiponectin and reduced leptin concentrations in both plasma and adipose tissue; it did not elevate plasma insulin concentration compared to the high-fat corn oil diet. All high-fat diets elevated concentrations of plasminogen activator inhibitor-1 (PAI-1) and monocyte chemoattractant protein-1 (MCP-1) but lowered resistin concentrations in both plasma and adipose tissue. In conclusion, fish oil may be beneficial in improving insulin sensitivity by upregulation of adiponectin and downregulation of leptin production; n3 and n6 PUFA do not play a role at the dietary levels tested in reducing adiposity and production of pro-inflammatory cytokines (leptin, PAI-1, MCP-1 and resistin) and anti-inflammatory cytokine adiponectin. C1 [Sundaram, Sneha; Bukowski, Michael R.; Picklo, Matthew J.; Yan, Lin] ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58202 USA. [Lie, Wen-Rong] EMD Millipore Corp, St Charles, MO 63304 USA. RP Yan, L (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58202 USA. EM lin.yan@ars.usda.gov OI yan, lin/0000-0003-3646-6374 NR 52 TC 5 Z9 5 U1 4 U2 5 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD MAY PY 2016 VL 51 IS 5 BP 571 EP 582 DI 10.1007/s11745-015-4093-x PG 12 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DL0NE UT WOS:000375328900007 PM 26645280 ER PT J AU Raatz, SK Johnson, LK Bukowski, MR AF Raatz, Susan K. Johnson, LuAnn K. Bukowski, Michael R. TI Enhanced Bioavailability of EPA From Emulsified Fish Oil Preparations Versus Capsular Triacylglycerol SO LIPIDS LA English DT Article; Proceedings Paper CT Northern Great Plains Lipid Conference CY MAY 31-JUN 01, 2015 CL Univ Manitoba, Winnipeg, CANADA HO Univ Manitoba DE Fish oil; Phospholipid fatty acids; Chylomicrons ID POLYUNSATURATED FATTY-ACIDS; DOCOSAHEXAENOIC ACID; CARDIOVASCULAR-DISEASE; HEALTH-BENEFITS; OMEGA-3-FATTY-ACIDS; PLASMA; LIPIDS; SUPPLEMENTATION; METABOLISM; HUMANS AB For those individuals who are unable to consume adequate long chain omega-3 fatty acids (LCn3) from dietary sources, fish oil supplementation is an attractive alternative Pre-emulsified fish oil supplements, an alternative to capsular triacylglycerol, may enhance the uptake of LCn3 fatty acids it contains. A randomized, Latin-square crossover design was used to compare the effects of four fish oil supplement preparations (Emulsions S, B and N) on phospholipid fatty acid (PLFA) concentrations in ten healthy volunteers compared to oil capsules over 48 h after a single dose and chylomicron fatty acid (CMFA) was evaluated over 8 h. Blood samples were collected at 0, 2, 4, 8, 24 and 48 h and fatty acid concentrations of PLFA and CMFA were determined by gas chromatography and the integrated area under the curve over 40 h ((i)AUC(0-48)) was determined. Emulsion S and Emulsion N promoted increased uptake of EPA into PLFA over 48 h when evaluating by (i)AUC(0-48) or individual time points of assessment. No differences were observed between supplements in the CMFA concentrations. C1 [Raatz, Susan K.; Johnson, LuAnn K.; Bukowski, Michael R.] ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA. [Raatz, Susan K.] Univ Minnesota, Dept Food Sci & Nutr, 1334 Eckles Ave, St Paul, MN USA. RP Raatz, SK (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, 2420 2nd Ave North, Grand Forks, ND 58203 USA.; Raatz, SK (reprint author), Univ Minnesota, Dept Food Sci & Nutr, 1334 Eckles Ave, St Paul, MN USA. EM susan.raatz@ars.usda.gov NR 29 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 EI 1558-9307 J9 LIPIDS JI Lipids PD MAY PY 2016 VL 51 IS 5 BP 643 EP 651 DI 10.1007/s11745-015-4100-2 PG 9 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA DL0NE UT WOS:000375328900013 PM 26688435 ER PT J AU Kern, RJ Lindholm-Perry, AK Freetly, HC Kuehn, LA Rule, DC Ludden, PA AF Kern, R. J. Lindholm-Perry, A. K. Freetly, H. C. Kuehn, L. A. Rule, D. C. Ludden, P. A. TI Rumen papillae morphology of beef steers relative to gain and feed intake and the association of volatile fatty acids with kallikrein gene expression SO LIVESTOCK SCIENCE LA English DT Article DE ADG; ADFI; Kallikrein; Papillae; Rumen; Volatile fatty acids ID DIET; EFFICIENCY; CATTLE; COWS AB Feed costs are the most expensive input in beef production. Improvement in the ability of beef cattle to convert feed into meat would lower feed inputs and reduce the cost of production. The rumen epithelium is responsible for absorption and metabolism of nutrients and microbial by-products, and may play a significant role in gain or feed intake. Our objective was to determine the relationships among rumen papillae morphology, gene expression, volatile fatty acid concentrations, and gain and feed intake. Average daily gain (ADG) and average daily feed intake (ADFI) were collected on a crossbred population of beef steers over three feeding trials. Based on feed intake and weight gain differences, 48 steers were selected for the project (16 from each feeding study). At harvest, rumen epithelial samples were taken from three locations in the rumen of each animal. The number of papillae on 1 cm(2) of epithelium was counted to determine density. Papillae (n=30) from each sample were measured for length and width. The density, length, and width were combined to determine surface enlargement factor (SEF). None of the morphological characteristics of the papillae (length, width, density or SEF) were associated with feed intake or gain (P >= 0.10). Ruminal fluid was collected from steers (n=15) in the third trial for volatile fatty acid (VFA) analysis to determine if a relationship between VFA and ADG or ADFI existed. No differences in volatile fatty acid (VFA) concentrations were associated (P >= 0.17) with ADFI or ADG. VFA variation was also evaluated for a relationship with kallikrein (KLK) genes since ruminal butyrate concentrations have previously been associated with the transcript abundance KLK genes. Additionally, qRTPCR data showed that variation in the transcript abundance of KLK6,8-10, and 13 associated with feed intake in our first trial of steers. Thus, we evaluated the transcript abundance of eight KLK genes in rumen papillae from steers in the third trial (n=16). Expression levels of KLK5 correlated with valerate concentrations in the rumen (P < 0.05), KLK10 correlated with acetate concentrations in the rumen (P < 0.05), KLK12 correlated with acetate and butyrate concentrations in the rumen (P < 0.05), and the expression levels of KLK9 and KLK10 were associated with gain. While rumen papillae morphology was not associated with beef steer gain or intake, our data suggests members of the kallikrein gene family have a relationship with the VFA environment in the rumen, and also appear to play a role in the highly correlated traits of ADG and ADFI. Published by Elsevier B.V. C1 [Kern, R. J.; Rule, D. C.; Ludden, P. A.] Univ Wyoming, Dept Anim Sci, Laramie, WY 82070 USA. [Kern, R. J.; Lindholm-Perry, A. K.; Freetly, H. C.; Kuehn, L. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Kern, RJ (reprint author), Univ Wyoming, Dept Anim Sci, Laramie, WY 82070 USA.; Kern, RJ (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Rebecca.Kern@ARS.USDA.GOV NR 23 TC 0 Z9 0 U1 3 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD MAY PY 2016 VL 187 BP 24 EP 30 DI 10.1016/j.livsci.2016.02.007 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA DL2YO UT WOS:000375501300004 ER PT J AU Burbank, LP Stenger, DC AF Burbank, Lindsey P. Stenger, Drake C. TI A Temperature-Independent Cold-Shock Protein Homolog Acts as a Virulence Factor in Xylella fastidiosa SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID OXIDATIVE STRESS TOLERANCE; LEAF SCORCH DISEASE; BOTULINUM ATCC 3502; ESCHERICHIA-COLI; CSPA-FAMILY; BACILLUS-SUBTILIS; PIERCES-DISEASE; LISTERIA-MONOCYTOGENES; PSEUDOMONAS-SYRINGAE; REACTIVE OXYGEN AB Xylella fastidiosa, causal agent of Pierce's disease (PD) of grapevine, is a fastidious organism that requires very specific conditions for replication and plant colonization. Cold temperatures reduce growth and survival of X. fastidiosa both in vitro and in planta. However, little is known regarding physiological responses of X. fastidiosa to temperature changes. Cold-shock proteins (CSP), a family of nucleic acid binding proteins, act as chaperones facilitating translation at low temperatures. Bacterial genomes often encode multiple CSP, some of which are strongly induced following exposure to cold. Additionally, CSP contribute to the general stress response through mRNA stabilization and posttranscriptional regulation. A putative CSP homolog (Csp1) with RNA-binding activity was identified in X. fastidiosa Stag's Leap. The csp1 gene lacked the long 5' untranslated region characteristic of cold-inducible genes and was expressed in a temperature-independent manner. As compared with the wild type, a deletion mutant of csp1 (Delta csp1) had decreased survival rates following cold exposure and salt stress in vitro. The deletion mutant also was significantly less virulent in grapevine, as compared with the wild type, in the absence of cold stress. These results suggest an important function of X. fastidiosa Csp1 in response to cellular stress and during plant colonization. C1 [Burbank, Lindsey P.; Stenger, Drake C.] USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. RP Burbank, LP (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM lindsey.burbank@ars.usda.gov FU United State Department of Agriculture (USDA) Agricultural Research Service [2034-22000-010-00D] FX We thank K. Zhang for technical support and M. S. Sisterson for statistical analysis. Funding for this work was from United State Department of Agriculture (USDA) Agricultural Research Service appropriated project 2034-22000-010-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not constitute endorsement by USDA. USDA is an equal opportunity provider and employer. NR 75 TC 3 Z9 3 U1 6 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD MAY PY 2016 VL 29 IS 5 BP 335 EP 344 DI 10.1094/MPMI-11-15-0260-R PG 10 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA DK6GU UT WOS:000375021500001 PM 26808446 ER PT J AU Akbar, MA Cao, JJ Lu, YQ Nardo, D Chen, MJ Elshikha, AS Ahamed, R Brantly, M Chang, LJ Holliday, LS Song, SH AF Akbar, Mohammad A. Cao, Jay J. Lu, Yuanqing Nardo, David Chen, Mong-Jen Elshikha, Ahmed S. Ahamed, Rubina Brantly, Mark Chang, Lung-ji Holliday, Lexie Shannon Song, Sihong TI Alpha-1 Antitrypsin Gene Therapy Prevented Bone Loss in an Ovariectomy Induced Osteoporosis Mouse Model SO MOLECULAR THERAPY LA English DT Meeting Abstract CT 19th Annual Meeting of the American-Society-of-Gene-and-Cell-Therapy (ASGCT) CY MAY 04-07, 2016 CL Washington, DC SP Amer Soc Gene & Cell Therapy C1 [Akbar, Mohammad A.; Nardo, David; Chen, Mong-Jen; Elshikha, Ahmed S.; Ahamed, Rubina; Song, Sihong] Univ Florida, Pharmaceut, Gainesville, FL USA. [Cao, Jay J.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Lu, Yuanqing; Brantly, Mark] Univ Florida, Med, Gainesville, FL USA. [Chang, Lung-ji] Univ Florida, Mol Genet & Microbiol, Gainesville, FL USA. [Holliday, Lexie Shannon] Univ Florida, Orthodont, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1525-0016 EI 1525-0024 J9 MOL THER JI Mol. Ther. PD MAY PY 2016 VL 24 SU 1 MA 632 BP S250 EP S250 PG 1 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA DK9PT UT WOS:000375264200623 ER PT J AU Kamath, PL Foster, JT Drees, KP Luikart, G Quance, C Anderson, NJ Clarke, PR Cole, EK Drew, ML Edwards, WH Rhyan, JC Treanor, JJ Wallen, RL White, PJ Robbe-Austerman, S Cross, PC AF Kamath, Pauline L. Foster, Jeffrey T. Drees, Kevin P. Luikart, Gordon Quance, Christine Anderson, Neil J. Clarke, P. Ryan Cole, Eric K. Drew, Mark L. Edwards, William H. Rhyan, Jack C. Treanor, John J. Wallen, Rick L. White, Patrick J. Robbe-Austerman, Suelee Cross, Paul C. TI Genomics reveals historic and contemporary transmission dynamics of a bacterial disease among wildlife and livestock SO NATURE COMMUNICATIONS LA English DT Article ID YELLOWSTONE-NATIONAL-PARK; BISON BISON-BISON; FREE-RANGING ELK; POPULATION-DYNAMICS; EVOLUTIONARY DYNAMICS; MOLECULAR EVOLUTION; UNITED-STATES; RABIES VIRUS; BRUCELLOSIS; TIME AB Whole-genome sequencing has provided fundamental insights into infectious disease epidemiology, but has rarely been used for examining transmission dynamics of a bacterial pathogen in wildlife. In the Greater Yellowstone Ecosystem (GYE), outbreaks of brucellosis have increased in cattle along with rising seroprevalence in elk. Here we use a genomic approach to examine Brucella abortus evolution, cross-species transmission and spatial spread in the GYE. We find that brucellosis was introduced into wildlife in this region at least five times. The diffusion rate varies among Brucella lineages (similar to 3 to 8 km per year) and over time. We also estimate 12 host transitions from bison to elk, and 5 from elk to bison. Our results support the notion that free-ranging elk are currently a self-sustaining brucellosis reservoir and the source of livestock infections, and that control measures in bison are unlikely to affect the dynamics of unrelated strains circulating in nearby elk populations. C1 [Kamath, Pauline L.; Cross, Paul C.] US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Foster, Jeffrey T.; Drees, Kevin P.] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA. [Luikart, Gordon] Univ Montana, Flathead Lake Biol Stn, Div Biol Sci, Missoula, MT 59812 USA. [Quance, Christine; Robbe-Austerman, Suelee] USDA APHIS, Natl Vet Serv Labs, Ames, IA 50010 USA. [Anderson, Neil J.] Montana Fish Wildlife & Pk, Bozeman, MT 59718 USA. [Clarke, P. Ryan; Rhyan, Jack C.] USDA APHIS, Vet Serv, Ft Collins, CO 80526 USA. [Cole, Eric K.] USFWS, Natl Elk Refuge, Jackson, WY 83001 USA. [Drew, Mark L.] Idaho Dept Fish & Game, Wildlife Hlth Lab, Caldwell, ID 83607 USA. [Edwards, William H.] Wyoming Game & Fish Dept, Laramie, WY 82070 USA. [Treanor, John J.; Wallen, Rick L.; White, Patrick J.] Natl Pk Serv, Yellowstone Natl Pk, Mammoth, WY 82190 USA. [Foster, Jeffrey T.; Drees, Kevin P.] Univ New Hampshire, Dept Mol Cellular & Biomed Sci, Durham, NH 03824 USA. RP Kamath, PL (reprint author), US Geol Survey, Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. EM pkamath@usgs.gov RI Cross, Paul/K-6987-2012; OI Cross, Paul/0000-0001-8045-5213; Foster, Jeffrey/0000-0001-8235-8564 FU National Science Foundation-Ecology and Evolution of Infectious Diseases [DEB-1067129]; New Hampshire Agricultural Experiment Station [2664]; USDA National Institute of Food and Agriculture Hatch Project [1007917]; U.S. Geological Survey; U.S. Department of Agriculture FX We thank the Wyoming Game and Fish Department, Montana Fish Wildlife and Parks, Idaho Department of Fish and Game, U.S. Fish and Wildlife Service and National Park Service for providing B. abortus isolates and associated data from wildlife. Slaughterhouse owners provided access to their facilities for sampling, and Claudia Almandra, Mike O'Brien, Chris Geremia, Doug Blanton, Troy Davis and the Stephen's Creek staff helped obtain wildlife tissues. The National Veterinary Services Laboratories provided livestock data. We are also grateful to Roman Biek for providing an insightful review as part of the USGS Fundamental Science Practices; Philippe Lemey, Samantha Lycett, Roland Kao, Julio Benavides and Tod Stuber for technical assistance; Angela Brennan, Emily Almberg and Raina Plowright for helpful comments on earlier drafts. Funding was provided by the U.S. Geological Survey, U.S. Department of Agriculture and National Science Foundation-Ecology and Evolution of Infectious Diseases (DEB-1067129). Partial funding was provided by the New Hampshire Agricultural Experiment Station (Scientific Contribution Number 2664). This work was supported by the USDA National Institute of Food and Agriculture Hatch Project (1007917). NR 70 TC 4 Z9 4 U1 8 U2 21 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAY PY 2016 VL 7 AR 11448 DI 10.1038/ncomms11448 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL2VI UT WOS:000375492900001 PM 27165544 ER PT J AU Martin, G Leivar, P Ludevid, D Tepperman, JM Quail, PH Monte, E AF Martin, Guiomar Leivar, Pablo Ludevid, Dolores Tepperman, James M. Quail, Peter H. Monte, Elena TI Phytochrome and retrograde signalling pathways converge to antagonistically regulate a light-induced transcriptional network SO NATURE COMMUNICATIONS LA English DT Article ID NUCLEAR GENE-EXPRESSION; ARABIDOPSIS-THALIANA; CHLOROPLAST DEVELOPMENT; PLASTID SIGNALS; HIGHER-PLANTS; PIF3; PHOTOMORPHOGENESIS; RESPONSES; SEEDLINGS; MUTANTS AB Plastid-to-nucleus retrograde signals emitted by dysfunctional chloroplasts impact photomorphogenic development, but the molecular link between retrograde-and photosensory-receptor signalling has remained unclear. Here, we show that the phytochrome and retrograde signalling (RS) pathways converge antagonistically to regulate the expression of the nuclear-encoded transcription factor GLK1, a key regulator of a light-induced transcriptional network central to photomorphogenesis. GLK1 gene transcription is directly repressed by PHYTOCHROME-INTERACTING FACTOR (PIF)-class bHLH transcription factors in darkness, but light-activated phytochrome reverses this activity, thereby inducing expression. Conversely, we show that retrograde signals repress this induction by a mechanism independent of PIF mediation. Collectively, our data indicate that light at moderate levels acts through the plant's nuclear-localized sensory-photoreceptor system to induce appropriate photomorphogenic development, but at excessive levels, sensed through the separate plastid-localized RS system, acts to suppress such development, thus providing a mechanism for protection against photo-oxidative damage by minimizing the tissue exposure to deleterious radiation. C1 [Martin, Guiomar; Leivar, Pablo; Ludevid, Dolores; Monte, Elena] CSIC IRTA UAB UB, Ctr Res Agr Genom, Dept Mol Genet, Barcelona 08193, Spain. [Leivar, Pablo] IQS Sch Engn, Dept Bioengn, Barcelona 08017, Spain. [Tepperman, James M.; Quail, Peter H.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Tepperman, James M.; Quail, Peter H.] ARS, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Monte, E (reprint author), CSIC IRTA UAB UB, Ctr Res Agr Genom, Dept Mol Genet, Barcelona 08193, Spain. EM elena.monte@cragenomica.es RI Elena, Monte/I-7221-2015; Leivar, Pablo/D-7808-2017; OI Leivar, Pablo/0000-0003-4878-3684; Martin Matas, Guiomar/0000-0001-6642-1666 FU Spanish 'Ministerio de Ciencia e Innovacion' [BIO2009-07675]; Ministerio de Economia [BIO2012-31672, BIO2012-31860]; Ministerio de Educacion, Cultura y Deporte [PRX12/00631]; Generalitat de Catalunya [2009-SGR-206]; 'Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa' fellowship from the Generalitat de Catalunya (Beatriu de Pinos programme); Marie Curie IRG grant [PIRG06-GA-2009-256420]; National Institutes of Health (NIH) [2R01 GM-047475]; USDA ARS Current Research Information System [5335-21000-032-00D] FX We thank J. Langdale, J. Chory, Q. Xie, N. Sanchez-Coll and G. Toledo-Ortiz for materials, and M. Rodriguez-Concepcion and G. Toledo-Ortiz for comments on the manuscript. We also thank the European Arabidopsis Biological Stock Center for the Arabidopsis T-DNA insertion lines. This work was supported by grants from the Spanish 'Ministerio de Ciencia e Innovacion' BIO2009-07675, 'Ministerio de Economia' BIO2012-31672, 'Ministerio de Educacion, Cultura y Deporte' PRX12/00631, and from the Generalitat de Catalunya 2009-SGR-206 to E.M.; by 'Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa' fellowship from the Generalitat de Catalunya (Beatriu de Pinos programme) and Marie Curie IRG PIRG06-GA-2009-256420 grant to P.L.; by grant from 'Ministerio de Economia' BIO2012-31860 to D.L.; and by grants from the National Institutes of Health (NIH; 2R01 GM-047475) and USDA ARS Current Research Information System (5335-21000-032-00D) to P.H.Q. NR 55 TC 7 Z9 7 U1 8 U2 22 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD MAY PY 2016 VL 7 AR 11431 DI 10.1038/ncomms11431 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL2VA UT WOS:000375492100001 PM 27150909 ER PT J AU Verma, SK Calero-Bernal, R Cerqueira-Cezar, CK Kwok, OCH Dudley, M Jiang, TT Su, CL Hill, D Dubey, JP AF Verma, Shiv Kumar Calero-Bernal, Rafael Cerqueira-Cezar, Camila K. Kwok, Oliver C. H. Dudley, Mike Jiang, Tiantian Su, Chunlei Hill, Dolores Dubey, Jitender P. TI Toxoplasmosis in geese and detection of two new atypical Toxoplasma gondii strains from naturally infected Canada geese (Branta canadensis) SO PARASITOLOGY RESEARCH LA English DT Article DE Canada geese; Toxoplasma gondii; Bioassay; Genotype ID SEROLOGICAL SURVEY; ANSER-DOMESTICA; RISK-FACTORS; SEROPREVALENCE; CHICKENS; CHINA; BIRDS; IDENTIFICATION; PATTERNS; OOCYSTS AB Wild birds are important in the epidemiology of toxoplasmosis because they can serve as reservoir hosts, and vectors of zoonotic pathogens including Toxoplasma gondii. Canada goose (Branta canadensis) is the most widespread geese in North America. Little is known concerning T. gondii infection in both migratory, and local resident populations of Canada geese. Here, we evaluated the seroprevalence, isolation, and genetic characterization of viable T. gondii isolates from a migratory population of Canada geese. Antibodies against T. gondii were detected in 12 of 169 Canada geese using the modified agglutination test (MAT, cutoff 1:25). The hearts of 12 seropositive geese were bioassayed in mice for isolation of T. gondii. Viable parasites were isolated from eight. One isolate was obtained from a seropositive goose by both bioassays in mice, and in a cat; the cat fed infected heart excreted T. gondii oocysts. Additionally, one isolate was obtained from a pool of four seronegative (< 1:25) geese by bioassay in a cat. The T. gondii isolates were further propagated in cell culture, and DNA extracted from cell culture-derived tachyzoites were characterized using 10 polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genetic markers (SAG1, 5' and 3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). The results revealed five different genotypes. ToxoDB PCR-RFLP genotype #1 (type II) in one isolate, genotype #2 (type III) in four isolates, genotype #4 in two isolates, and two new genotypes (ToxoDB PCR-RFLP genotype #266 in one isolate and #267 in one isolate) were identified. These results indicate genetic diversity of T. gondii strains in the Canada geese, and this migratory bird might provide a mechanism of T. gondii transmission at great distances from where an infection was acquired. C1 [Verma, Shiv Kumar; Calero-Bernal, Rafael; Cerqueira-Cezar, Camila K.; Kwok, Oliver C. H.; Hill, Dolores; Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. [Dudley, Mike] ARS, USDA, Beltsville Agr Res Ctr, Farm Serv, Bldg 513, Beltsville, MD 20705 USA. [Jiang, Tiantian; Su, Chunlei] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov NR 27 TC 1 Z9 1 U1 6 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD MAY PY 2016 VL 115 IS 5 BP 1767 EP 1772 DI 10.1007/s00436-016-4914-8 PG 6 WC Parasitology SC Parasitology GA DK5RG UT WOS:000374976900003 PM 26796021 ER PT J AU Mahaffee, WF Stoll, R AF Mahaffee, Walter F. Stoll, Rob TI The Ebb and Flow of Airborne Pathogens: Monitoring and Use in Disease Management Decisions SO PHYTOPATHOLOGY LA English DT Review ID REAL-TIME PCR; GRAPEVINE POWDERY MILDEW; LONG-DISTANCE TRANSPORT; POLYMERASE-CHAIN-REACTION; BOTRYTIS LEAF-BLIGHT; ON-A-CHIP; PLANT-PATHOGENS; FUSARIUM-GRAMINEARUM; QUANTITATIVE PCR; AERIAL DISPERSAL AB Perhaps the earliest form of monitoring the regional spread of plant disease was a group of growers gathering together at the market and discussing what they see in their crops. This type of reporting continues to this day through regional extension blogs, by crop consultants and more formal scouting of sentential plots in the IPM PIPE network (http://www.ipmpipe.org/). As our knowledge of plant disease epidemiology has increased, we have also increased our ability to detect and monitor the presence of pathogens and use this information to make management decisions in commercial production systems. The advent of phylogenetics, next-generation sequencing, and nucleic acid amplification technologies has allowed for development of sensitive and accurate assays for pathogen inoculum detection and quantification. The application of these tools is beginning to change how we manage diseases with airborne inoculum by allowing for the detection of pathogen Movement instead of assuming it and by targeting management strategies to the early phases of the epidemic development when there is the greatest opportunity to reduce the rate of disease development. While there are numerous advantages to using data on inoculum presence to aid management decisions, there are limitations in what the data represent that are often unrecognized. In addition, our understanding of where and how to effectively monitor airborne inoculum is limited. There is a strong need to improve our knowledge of the mechanisms that influence inoculum dispersion across scales as particles move from leaf to leaf, and everything in between. C1 [Mahaffee, Walter F.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Stoll, Rob] Univ Utah, Dept Mech Engn, Salt Lake City, UT 84112 USA. RP Mahaffee, WF (reprint author), USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. EM walt.mahaffee@ars.usda.gov FU American Vineyard Foundation; National Science Foundation [AGS-1255662]; Oregon Wine Board; Viticulture Consortium West; University of Utah; USDA-ARS CRIS [5358-22000-0390-OOD] FX This work was supported by American Vineyard Foundation, National Science Foundation (AGS-1255662), Oregon Wine Board, Viticulture Consortium West, University of Utah, and USDA-ARS CRIS 5358-22000-0390-OOD. We thank T. Neill, L. Thiessen, A. Peetz, D. Martin, J. Keune, and D. Gent for discussion and refining our ideas on molecular detection and quantification and E. Pardyjak, N. Miller, B. Baily, and T. Price for their insight into turbulent flows and particle dispersion. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 140 TC 2 Z9 2 U1 9 U2 19 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2016 VL 106 IS 5 BP 420 EP 431 DI 10.1094/PHYTO-02-16-0060-RVW PG 12 WC Plant Sciences SC Plant Sciences GA DL0YC UT WOS:000375357500001 PM 27003505 ER PT J AU Strausbaugh, CA AF Strausbaugh, Carl A. TI Leuconostoc spp. Associated with Root Rot in Sugar Beet and Their Interaction with Rhizoctonia solani SO PHYTOPATHOLOGY LA English DT Article ID MESENTEROIDES SUBSP MESENTEROIDES; BACTERIAL VASCULAR NECROSIS; PSEUDOMONAS SP CMR12A; CROWN ROT; ANTAGONISTIC BACTERIA; MICROBIAL COMMUNITIES; PHYLOGENETIC ANALYSIS; SOIL SUPPRESSIVENESS; NATURAL SUPPRESSION; INTERMOUNTAIN WEST AB Rhizoctonia root and crown rot is an important disease problem in sugar beet caused by Rhizoctonia solani and also shown to be associated with Leuconostoc spp. Initial Leuconostoc studies were conducted with only a few isolates and the relationship of Leuconostoc with R. solani is poorly understood; therefore, a more thorough investigation was conducted. In total, 203 Leuconostoc isolates were collected from recently harvested sugar beet roots in southern Idaho and southeastern Oregon during 2010 and 2012: 88 and 85% Leuconostoc mesenteroides, 6 and 15% L. pseudomesenteroides, 2 and 0% L. kimchi, and 4 and 0% unrecognized Leuconostoc spp., respectively. Based on 16S ribosomal RNA sequencing, haplotype 11 (L. mesenteroides isolates) comprised 68 to 70% of the isolates in both years. In pathogenicity field studies with commercial sugar beet 'B-7', all Leuconostoc isolates caused more rot (P < 0.0001; alpha = 0.05) when combined with R. solani than when inoculated alone in both years. Also, 46 of the 52 combination treatments over the 2 years had significantly more rot (P < 0.0001; alpha = 0.05) than the fungal check. The data support the conclusion that a synergistic interaction leads to more rot when both Leuconostoc spp. and R. solani are present in sugar beet roots. C1 [Strausbaugh, Carl A.] USDA ARS, NWISRL, 3793 North 3600 East, Kimberly, ID 83341 USA. RP Strausbaugh, CA (reprint author), USDA ARS, NWISRL, 3793 North 3600 East, Kimberly, ID 83341 USA. EM carl.strausbaugh@ars.usda.gov OI Strausbaugh, Carl/0000-0001-6773-1375 FU United States Department of Agriculture CRIS project [5368-21220-003-00D]; Amalgamated Sugar Co., LLC; Beet Sugar Development Foundation; Snake River sugar beet growers FX These data support the objectives for the United States Department of Agriculture CRIS project 5368-21220-003-00D. We thank the Amalgamated Sugar Co., LLC; Beet Sugar Development Foundation; and Snake River sugar beet growers for supporting our research work; and J. Reed, D. Kenney, and T. Keeth for their technical support efforts. Mention of trade names or commercial products in this article is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 88 TC 0 Z9 0 U1 7 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2016 VL 106 IS 5 BP 432 EP 441 DI 10.1094/PHYTO-12-15-0325-R PG 10 WC Plant Sciences SC Plant Sciences GA DL0YC UT WOS:000375357500002 PM 26735061 ER PT J AU Hilf, ME Lewis, RS AF Hilf, Mark E. Lewis, Reid S. TI Transmission and Propagation of 'Candidatus Liberibacter asiaticus' by Grafting with Individual Citrus Leaves SO PHYTOPATHOLOGY LA English DT Article ID CA. L. AMERICANUS; HUANGLONGBING DISEASE; SWEET ORANGE; HEMIPTERA PSYLLIDAE; 1ST REPORT; INFECTION; TOLERANT; BRAZIL; TRANSCRIPTOME; COLONIZATION AB Huanglongbing (HLB) is a chronic, progressive decline disease in citrus associated with a systemic infection by the bacterium 'Candidatus Liberibacter asiaticus'. Transmission of the bacterium in the field is by the Asian citrus psyllid, Diaphorina citri Kuwayama. Experimental propagation of 'Ca. L. asiaticus' is done primarily by grafting pieces of bud wood from an infected plant. To produce a small-scale model system for investigation of pathogen biology, we investigated grafting single leaves from infected citrus plants as sources of inoculum for propagation of the bacterium. In total, 162 plants ranging in age from 3 to 18 months were grafted. Grafting with intact asymptomatic and HLB-symptomatic leaves resulted in 61 of 78 (78%) and 35 of 41 (85%) of the plants infected with 'Ca. L. asiaticus', respectively. Inoculum consisting of the leaf petiole only or only an inoculum tissue remnant under the bark of the receptor tree resulted in 6 of 12 (50%) and 7 of 31 (23%) infected trees, respectively. Real-time polymerase chain reaction (qPCR) assays verified the infection in plants, a majority of which developed the foliar blotchy mottle symptom considered diagnostic for HLB, while some plants also displayed the stunted, chlorotic shoots for which the disease is named. The qPCR data together with the symptoms displayed demonstrated that individual leaves from infected trees can serve as effective inoculum sources for transmission and propagation of 'Ca. L. asiaticus' via grafting. C1 [Hilf, Mark E.; Lewis, Reid S.] USDA ARS, 2001 S Rock Rd, Ft Pierce, FL USA. RP Hilf, ME (reprint author), USDA ARS, 2001 S Rock Rd, Ft Pierce, FL USA. EM Mark.Hilf@ars.usda.gov NR 46 TC 1 Z9 1 U1 6 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2016 VL 106 IS 5 BP 452 EP 458 DI 10.1094/PHYTO-09-15-0221-R PG 7 WC Plant Sciences SC Plant Sciences GA DL0YC UT WOS:000375357500004 PM 26807818 ER PT J AU Jaaffar, AKM Paulitz, TC Schroeder, KL Thomashow, LS Weller, DM AF Jaaffar, Ahmad Kamil Mohd Paulitz, Timothy C. Schroeder, Kurtis L. Thomashow, Linda S. Weller, David M. TI Molecular Characterization, Morphological Characteristics, Virulence, and Geographic Distribution of Rhizoctonia spp. in Washington State SO PHYTOPATHOLOGY LA English DT Article ID POLYMERASE-CHAIN-REACTION; ROOT-ROT; SOLANI AG-8; PACIFIC-NORTHWEST; WINTER-WHEAT; BARE PATCH; ANASTOMOSIS GROUPS; BINUCLEATE RHIZOCTONIA; AGRICULTURAL SOILS; WESTERN-AUSTRALIA AB Rhizoctonia root rot and bare patch, caused by Rhizoctonia solani anastomosis group (AG)-8 and R. oryzae, are chronic and important yield-limiting diseases of wheat and barley in the Inland Pacific Northwest (PNW) of the United States. Major gaps remain in our understanding of the epidemiology of these diseases, in part because multiple Rhizoctonia AGs and species can be isolated from the same cereal roots from the field, contributing to the challenge of identifying the causal agents correctly. In this study, a collection totaling 498 isolates of Rhizoctonia was assembled from surveys conducted from 2000 to 2009, 2010, and 2011 over a wide range of cereal production fields throughout Washington State in the PNW. To determine the identity of the isolates, PCR with AG- or species specific primers and/or DNA sequence analysis of the internal transcribed spacers was performed. R. solani AG-2-1, AG-8, AG-10, AG-3, AG-4, and AG-11 comprised 157 (32%), 70 (14%), 21 (4%), 20 (4%), 1 (0.2%), and 1 (0.2%), respectively, of the total isolates. AG-I-like binucleate Rhizoctonia sp. comprised 44 (9%) of the total; and 53 (11%), 80 (16%), and 51 (10%) were identified as R. oryzae genotypes I, II, and III, respectively. Isolates of AG-2-1, the dominant Rhizoctonia, occurred in all six agronomic zones defined by annual precipitation and temperature within the region sampled. Isolates of AG-8 also were cosmopolitan in their distribution but the frequency of isolation varied among years, and they were most abundant in zones of low and moderate precipitation. R. oryzae was cosmopolitan, and collectively the three genotypes comprised 37% of the isolates. Only isolates of R. solani AG-8 and R. oryzae genotypes II and III (but not genotype I) caused symptoms typically associated with Rhizoctonia root rot and bare patch of wheat. Isolates of AG-2-1 caused only mild root rot and AG-I-like binucleate isolates and members of groups AG-3, AG-4, and AG-11 showed only slight or no discoloration of the roots. However, all isolates of AG-2-1 caused severe damping-off of canola, resulting in 100% mortality. Isolates of Rhizoctonia AG-8, AG-2-1, AG-10, AG-I-like binucleate Rhizoctonia, and R. oryzae genotypes I, II, and III could be distinguished by colony morphology on potato dextrose agar, by PCR with specific primers, or by the type and severity of disease on wheat and canola seedlings, and results of these approaches correlated completely. Based on cultured isolates, we also identified the geographic distribution of all of these Rhizoctonia isolates in cereal-based production systems throughout Washington State. C1 [Jaaffar, Ahmad Kamil Mohd; Schroeder, Kurtis L.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Paulitz, Timothy C.; Thomashow, Linda S.; Weller, David M.] Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. [Jaaffar, Ahmad Kamil Mohd] Malaysian Cocoa Board, Biotechnol Pk, Kota Samarahan 94300, Sarawak, Malaysia. [Schroeder, Kurtis L.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. RP Thomashow, LS; Weller, DM (reprint author), Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. EM linda.thomashow@ars.usda.gov; david.weller@ars.usda.gov OI Paulitz, Timothy/0000-0002-8885-3803 NR 73 TC 0 Z9 0 U1 5 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2016 VL 106 IS 5 BP 459 EP 473 DI 10.1094/PHYTO-09-15-0208-R PG 15 WC Plant Sciences SC Plant Sciences GA DL0YC UT WOS:000375357500005 PM 26780436 ER PT J AU Cooper, B Campbell, KB Beard, HS Garrett, WM Islam, N AF Cooper, Bret Campbell, Kimberly B. Beard, Hunter S. Garrett, Wesley M. Islam, Nazrul TI Putative Rust Fungal Effector Proteins in Infected Bean and Soybean Leaves SO PHYTOPATHOLOGY LA English DT Article ID TRITICINA PATHOGENICITY GENES; EXPRESSED SEQUENCE TAGS; MASS-SPECTROMETRY DATA; UROMYCES-APPENDICULATUS; PHAKOPSORA-PACHYRHIZI; FLAX RUST; IDENTIFICATION TECHNOLOGY; PROTEOMIC ANALYSIS; PLANT-CELLS; PHASEOLUS-VULGARIS AB The plant-pathogenic fungi Uromyces appendiculatus and Phakopsora pachyrhizi cause debilitating rust diseases on common bean and soybean. These rust fungi secrete effector proteins that allow them to infect plants, but their effector repertoires are not understood. The discovery of rust fungus effectors may eventually help guide decisions and actions that mitigate crop production loss. Therefore, we used mass spectrometry to identify thousands of proteins in infected beans and soybeans and in germinated fungal spores. The comparative analysis between the two helped differentiate a set of 24 U. appendiculatus proteins targeted for secretion that were specifically found in infected beans and a set of 34 U. appendiculatus proteins targeted for secretion that were found in germinated spores and infected beans. The proteins specific to infected beans included family 26 and family 76 glycoside hydrolases that may contribute to degrading plant cell walls. There were also several types of proteins with structural motifs that may aid in stabilizing the specialized fungal haustorium cell that interfaces the plant cell membrane during infection. There were 16 P. pachyrhizi proteins targeted for secretion that were found in infected soybeans, and many of these proteins resembled the U. appendiculatus proteins found in infected beans, which implies that these proteins are important to rust fungal pathology in general. This data set provides insight to the biochemical mechanisms that rust fungi use to overcome plant immune systems and to parasitize cells. C1 [Cooper, Bret; Campbell, Kimberly B.; Beard, Hunter S.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Garrett, Wesley M.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Islam, Nazrul] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Cooper, B (reprint author), USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. EM bret.cooper@ars.usda.gov FU U.S. Department of Agriculture-Agricultural Research Service FX We thank D. Luster for access to the plant pathogen containment facility at USDA-ARS, Ft. Detrick, MD; and X. Nou at USDA-ARS, Beltsville, MD, for use of his Omni Bead Raptor. This research was funded entirely by the U.S. Department of Agriculture-Agricultural Research Service. NR 74 TC 0 Z9 0 U1 9 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2016 VL 106 IS 5 BP 491 EP 499 DI 10.1094/PHYTO-11-15-0310-R PG 9 WC Plant Sciences SC Plant Sciences GA DL0YC UT WOS:000375357500008 PM 26780434 ER PT J AU Butnor, JR Samuelson, LJ Stokes, TA Johnsen, KH Anderson, PH Gonzalez-Benecke, CA AF Butnor, John R. Samuelson, Lisa J. Stokes, Thomas A. Johnsen, Kurt H. Anderson, Peter H. Gonzalez-Benecke, Carlos A. TI Surface-based GPR underestimates below-stump root biomass SO PLANT AND SOIL LA English DT Article DE GPR; Root mass; taproot; Lateral root; Below-stump; Pinus palustris; Longleaf pine ID GROUND-PENETRATING RADAR; TREE ROOTS; LOBLOLLY-PINE; UNITED-STATES; TAEDA; ALLOCATION; DIAMETERS; ECOSYSTEM; FORESTS; SYSTEMS AB While lateral root mass is readily detectable with ground penetrating radar (GPR), the roots beneath a tree (below-stump) and overlapping lateral roots near large trees are problematic for surface-based antennas operated in reflection mode. We sought to determine if tree size (DBH) effects GPR root detection proximal to longleaf pine (Pinus palustris Mill) and if corrections for could be applied to stand-level estimates of root mass. GPR (1500 MHz) was used to estimate coarse root mass proximal to 33 longleaf pine trees and compared to the amount of biomass excavated from pits proportional in area to tree basal diameter. Lateral roots were excavated to a depth of 1 m and taproots were excavated in their entirety. GPR underestimated longleaf pine below-stump mass and the magnitude of the underestimation increased with tree DBH. Non-linear regressions between GPR estimated root mass/excavated root mass and tree diameter at breast height (DBH) were highly significant for both below-stump (lateral + taproot) root mass (p < 0.0001, R-2 0.77) and lateral coarse root mass (p < 0.0001, R-2 0.65). GPR underestimates root mass proximal to trees, and this needs to be accounted for to accurately estimate stand-level belowground biomass. C1 [Butnor, John R.] Univ Vermont, USDA, Aiken Ctr, Forest Serv,Southern Res Stn, 81 Carrigan Dr,Room 208, Burlington, VT 05405 USA. [Johnsen, Kurt H.] USDA, Forest Serv, Southern Res Stn, 1577 Brevard Rd, Asheville, NC 28806 USA. [Samuelson, Lisa J.; Stokes, Thomas A.] Auburn Univ, Sch Forestry & Wildlife Sci, 3301 SFWS Bldg, Auburn, AL 36849 USA. [Anderson, Peter H.] USDA, Forest Serv, Southern Res Stn, 3041 East Cornwallis Rd, Durham, NC 27709 USA. [Gonzalez-Benecke, Carlos A.] Oregon State Univ, Coll Forestry, Dept Forest Engn Resources & Management, 280 Peavy Hall, Corvallis, OR 97331 USA. RP Butnor, JR (reprint author), Univ Vermont, USDA, Aiken Ctr, Forest Serv,Southern Res Stn, 81 Carrigan Dr,Room 208, Burlington, VT 05405 USA. EM jbutnor@fs.fed.us RI Butnor, John/P-9738-2016 FU U. S. Department of Defense through the Strategic Environmental Research and Development Program (SERDP) FX This research was supported by the U. S. Department of Defense through the Strategic Environmental Research and Development Program (SERDP). We appreciate the technical support provided by Jake Blackstock, Joel Burley, Thomas Christensen, Robert Eaton, Shelly Hooke, Jason Jackson, Lance Kress, John Lewis, Michael Rameriz, Justin Rathel and Karen Sarsony. We thank Dr. Frank Day, Old Dominion University, for reviewing this manuscript prior to submission. NR 40 TC 3 Z9 3 U1 4 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD MAY PY 2016 VL 402 IS 1-2 BP 47 EP 62 DI 10.1007/s11104-015-2768-y PG 16 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA DJ7PU UT WOS:000374404100003 ER PT J AU Elitzur, T Yakir, E Quansah, L Fei, ZJ Vrebalov, J Khayat, E Giovannoni, JJ Friedman, H AF Elitzur, Tomer Yakir, Esther Quansah, Lydia Fei Zhangjun Vrebalov, Julia Khayat, Eli Giovannoni, James J. Friedman, Haya TI Banana MaMADS Transcription Factors Are Necessary for Fruit Ripening and Molecular Tools to Promote Shelf-Life and Food Security SO PLANT PHYSIOLOGY LA English DT Article ID MADS-BOX GENES; ETHYLENE BIOSYNTHESIS; TOMATO; RIN; EXPRESSION; HOMOLOGS; REGULATOR; PROTEINS; QUALITY; SINGLE AB Genetic solutions to postharvest crop loss can reduce cost and energy inputs while increasing food security, especially for banana (Musa acuminata), which is a significant component of worldwide food commerce. We have functionally characterized two banana E class (SEPALLATA3 [SEP3]) MADS box genes, MaMADS1 and MaMADS2, homologous to the tomato (Solanum lycopersicum) RIN-MADS ripening gene. Transgenic banana plants repressing either gene (via antisense or RNA interference [RNAi]) were created and exhibited specific ripening delay and extended shelf-life phenotypes, including delayed color development and softening. The delay in fruit ripening is associated with a delay in climacteric respiration and reduced synthesis of the ripening hormone ethylene; in the most severe repressed lines, no ethylene was produced and ripening was most delayed. Unlike tomato rin mutants, banana fruits of all transgenic repression lines responded to exogenous ethylene by ripening normally, likely due to incomplete transgene repression and/or compensation by other MADS box genes. Our results show that, although MADS box ripening gene necessity is conserved across diverse taxa (monocots to dicots), unlike tomato, banana ripening requires at least two necessary members of the SEPALLATA MADS box gene group, and either can serve as a target for ripening control. The utility of such genes as tools for ripening control is especially relevant in important parthenocarpic crops such as the vegetatively propagated and widely consumed Cavendish banana, where breeding options for trait improvement are severely limited. C1 [Elitzur, Tomer; Yakir, Esther; Quansah, Lydia; Friedman, Haya] Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, IL-7510001 Bet Dagan, Israel. [Fei Zhangjun; Vrebalov, Julia; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [Fei Zhangjun; Vrebalov, Julia; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr, Ithaca, NY 14853 USA. [Khayat, Eli] Rahan Meristem Ltd, IL-22825 Rosh Hanikra, Western Galilee, Israel. RP Friedman, H (reprint author), Agr Res Org, Volcani Ctr, Dept Postharvest Sci Fresh Produce, IL-7510001 Bet Dagan, Israel. EM hayafr@agri.gov.il FU BARD; U.S.-Israel Binational Agricultural Research and Development Fund [IS-3803-05, IS-4371-10C]; U.S. Department of Agriculture-Agricultural Research Service FX This work was supported by BARD, the U.S.-Israel Binational Agricultural Research and Development Fund (grant nos. IS-3803-05 and IS-4371-10C), and the U.S. Department of Agriculture-Agricultural Research Service. This is contribution no. 735/15 from the Agriculture Research Center. NR 37 TC 1 Z9 1 U1 26 U2 45 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD MAY PY 2016 VL 171 IS 1 BP 380 EP 391 DI 10.1104/pp.15.01866 PG 12 WC Plant Sciences SC Plant Sciences GA DL1ZI UT WOS:000375431500029 PM 26956665 ER PT J AU Watkins, C Westphal, LM AF Watkins, Cristy Westphal, Lynne M. TI People Don't Talk in Institutional Statements: A Methodological Case Study of the Institutional Analysis and Development Framework SO POLICY STUDIES JOURNAL LA English DT Article DE ADICO syntax; institutional analysis and development; norms; ecological restoration; qualitative data ID GRAMMAR; NORMS; RESTORATION; MANAGEMENT; POLICY; RULES AB In this paper, we describe our application of Ostrom et al.'s ADICO syntax, a grammatical tool based in the Institutional Analysis and Development framework, to a study of ecological restoration decision making in the Chicago Wilderness region. As this method has only been used to look at written policy and/or extractive natural resource management systems, our application is novel in context (a value-adding environmental management action), data type (in-depth, qualitative interviews, and participant observation), and extent of institutional statement extraction (we extract rules, as well as norms and strategies). Through detailed description, visual aids, and case-specific qualitative examples, we show the usefulness of the ADICO syntax in detailing the full set of institutional statements-in-use: rules, norms, and strategies. One of the most interesting findings is that we found norms (and not just rules) to be prevalent and particularly meaningful guides for people's actions. This reinforces the need to address norms and strategies, not only rules, when developing effective policy. C1 [Watkins, Cristy] Univ Michigan, Sch Nat Resources & Environm, IFRI Int Forestry Resources & Inst Res Network, Ann Arbor, MI 48109 USA. [Westphal, Lynne M.] US Forest Serv, No Res Stn, Chicago, IL USA. RP Watkins, C (reprint author), Univ Michigan, Sch Nat Resources & Environm, IFRI Int Forestry Resources & Inst Res Network, Ann Arbor, MI 48109 USA. FU UK's Department for International Development (DFID) FX Cristy Watkins, PhD, is a post-doctoral research fellow with IFRI (International Forestry Resources and Institutions research network) at the School of Natural Resources and Environment, University of Michigan. She coordinates and conducts research for a project funded by the UK's Department for International Development (DFID) to evaluate the impacts of forest-sector environment and development programs, among other IFRI projects. NR 24 TC 1 Z9 1 U1 6 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0190-292X EI 1541-0072 J9 POLICY STUD J JI Policy Stud. J. PD MAY PY 2016 VL 44 SU 1 SI SI BP S98 EP S122 DI 10.1111/psj.12139 PG 25 WC Political Science; Public Administration SC Government & Law; Public Administration GA DK7ZJ UT WOS:000375145600006 ER PT J AU Gardner, NM Riley, RT Showker, JL Voss, KA Sachs, AJ Maddox, JR Gelineau-van Waes, JB AF Gardner, Nicole M. Riley, Ronald T. Showker, Jency L. Voss, Kenneth A. Sachs, Andrew J. Maddox, Joyce R. Gelineau-van Waes, Janee B. TI Elevated nuclear sphingoid base-1-phosphates and decreased histone deacetylase activity after fumonisin B1 treatment in mouse embryonic fibroblasts SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article DE Fumonisin B1; Histone deacetylase (HDAC); Histone acetylation; Neural tube defect (NTD); Sphinganine-1-phosphate (Sa1P) ID NEURAL-TUBE DEFECTS; VALPROIC ACID; FUSARIUM-MONILIFORME; KINASE-ACTIVITY; INDUCED TERATOGENESIS; GROWTH-FACTOR; SPINA-BIFIDA; IN-VIVO; SPHINGOSINE-1-PHOSPHATE; INHIBITION AB Fumonisin B1 (FBI) is a mycotoxin produced by a common fungal contaminant of corn. Administration of FB1 to pregnant LM/Bc mice induces exencephaly in embryos, and ingestion of FB1-contaminated food during early pregnancy is associated with increased risk for neural tube defects (NTDs) in humans. FB1 inhibits ceramide synthase enzymes in sphingolipid biosynthesis, causing sphinganine (Sa) and bioactive sphinganine-1-phosphate (Sa1P) accumulation in blood, cells, and tissues. Sphingosine kinases (Sphk) phosphorylate Sa to form Sa1P. Upon activation, Sphk1 associates primarily with the plasma membrane, while Sphk2 is found predominantly in the nucleus. In cells over-expressing Sphk2, accumulation of Sal Pin the nuclear compartment inhibits histone deacetylase (HDAC) activity, causing increased acetylation of histone lysine residues. In this study, FB1 treatment in LM/Bc mouse embryonic fibroblasts (MEFs) resulted in significant accumulation of Sal Pin nuclear extracts relative to cytoplasmic extracts. Elevated nuclear Sa1P corresponded to decreased histone deacetylase (HDAC) activity and increased histone acetylation at H2BK12, H3K9, H3K18, and H3K23. Treatment of LM/Bc MEFs with a selective Sphk1 inhibitor, PF-543, or with ABC294640, a selective Sphk2 inhibitor, significantly reduced nuclear Sa1P accumulation after FBI, although Sa1P levels remained significantly increased relative to basal levels. Concurrent treatment with both PF-543 and ABC294640 prevented nuclear accumulation of Sa1P in response to FB1. Other HDAC inhibitors are known to cause NTDs, so these results suggest that FBI-induced disruption of sphingolipid metabolism leading to nuclear Sa1P accumulation, HDAC inhibition, and histone hyperacetylation is a potential mechanism for FB1-induced NTDs. (C) 2016 Elsevier Inc. All rights reserved. C1 [Gardner, Nicole M.; Sachs, Andrew J.; Maddox, Joyce R.; Gelineau-van Waes, Janee B.] Creighton Univ, Sch Med, Dept Pharmacol, 2500 Calif Plaza, Omaha, NE 68178 USA. [Riley, Ronald T.; Showker, Jency L.; Voss, Kenneth A.] ARS, USDA, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. RP Gardner, NM (reprint author), Creighton Univ, Sch Med, Dept Pharmacol, 2500 Calif Plaza, Omaha, NE 68178 USA. EM nicolegardner@creighton.edu FU USDA-ARS NP108 in house project [6612-42000-012-00D]; Eunice Kennedy Shriver National Institute of Child Health & Human Development & NIH Office of the Director [RC4HD067971]; Nebraska Tobacco Settlement Biomedical Research Development Fund [LB692] FX This work was supported by USDA-ARS NP108 in house project 6612-42000-012-00D; RC4HD067971 Eunice Kennedy Shriver National Institute of Child Health & Human Development & NIH Office of the Director; and LB692 Nebraska Tobacco Settlement Biomedical Research Development Fund. NR 75 TC 1 Z9 1 U1 3 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0041-008X EI 1096-0333 J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD MAY 1 PY 2016 VL 298 BP 56 EP 65 DI 10.1016/j.taap.2016.02.018 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DJ7YN UT WOS:000374429200007 PM 26905748 ER PT J AU Sui, Y Wisniewski, M Droby, S Norelli, J Liu, J AF Sui, Yuan Wisniewski, Michael Droby, Samir Norelli, John Liu, Jia TI Recent advances and current status of the use of heat treatments in postharvest disease management systems: Is it time to turn up the heat? SO TRENDS IN FOOD SCIENCE & TECHNOLOGY LA English DT Review DE Biocontrol yeast; Fungal pathogen; Fruit; Heat treatment; -Omics; Postharvest system ID HOT-WATER TREATMENT; TREATMENT INDUCES RESISTANCE; YEAST CANDIDA-SAKE; PENICILLIUM-EXPANSUM; BIOLOGICAL-CONTROL; BOTRYTIS-CINEREA; STRESS TOLERANCE; CITRUS-FRUIT; BIOCONTROL EFFICACY; SODIUM-BICARBONATE AB Background: Postharvest diseases of harvested commodities cause significant reductions in food availability and financial profits. Additionally, regulatory agencies are increasingly restricting or banning the postharvest use of synthetic chemical fungicides. This has increased the need to develop more eco-friendly approaches to postharvest disease management, such as heat treatments and biological control using antagonistic yeasts. Scope and approach: The current review focuses on the physiological and molecular effects of heat treatments on the various components of postharvest disease management systems, namely, the commodity, fungal pathogen, and yeast biocontrol agent. The ability of postharvest heat treatments to induce host defense mechanisms and directly inhibit fungal pathogens is reviewed. The preconditioning of biocontrol yeasts by subjecting them to mild heat stress in order to induce cross-protection to abiotic stresses and to enhance biocontrol efficacy, is also discussed. The combined effects of postharvest heat treatments on disease control demonstrate its value as a key component of integrated approaches to postharvest disease management. Key findings and conclusions: A review of the literature indicates that heat treatments can induce host resistance, inhibit pathogen development, and enhance the efficacy of biocontrol agents by making them more resilient to an array of environmental stresses. Therefore, heat treatments should be considered as a key component of an integrated approach to postharvest disease management. Further research will be needed, however, to understand how to effectively adapt this technology for use on different commodities, pathogens, and biocontrol agents. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Sui, Yuan; Liu, Jia] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. [Wisniewski, Michael; Norelli, John] USDA ARS, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Droby, Samir] Agr Res Org, Volcani Ctr, IL-50250 Bet Dagan, Israel. RP Liu, J (reprint author), Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China. EM jialiu1983@163.com FU China Postdoctoral Science Foundation Funded Project [2015M570534]; National Natural Science Foundation of China [31501688]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry FX This work was supported by a China Postdoctoral Science Foundation Funded Project (2015M570534), the Project Supported by National Natural Science Foundation of China (31501688), and the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry to Yuan Sui. We thank the many scientists for their efforts and thoughtful discussions on this topic of this review, and apologize to the investigators whose specific results could not be cited due to space limitations but whose work framed the issues and ideas that are discussed. NR 75 TC 0 Z9 0 U1 9 U2 27 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0924-2244 J9 TRENDS FOOD SCI TECH JI Trends Food Sci. Technol. PD MAY PY 2016 VL 51 BP 34 EP 40 DI 10.1016/j.tifs.2016.03.004 PG 7 WC Food Science & Technology SC Food Science & Technology GA DL0PZ UT WOS:000375336300004 ER PT J AU Bischoff, KM Zhang, YH Rich, JO AF Bischoff, Kenneth M. Zhang, Yanhong Rich, Joseph O. TI Fate of virginiamycin through the fuel ethanol production process SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Antibiotics; Distillers grains; Fuel ethanol; Virginiamycin ID LACTIC-ACID BACTERIA; ALCOHOL FERMENTATION; YEAST; ENTEROCOCCUS; ANTIBIOTICS; RESISTANCE; GROWTH AB Antibiotics are frequently used to prevent and treat bacterial contamination of commercial fuel ethanol fermentations, but there is concern that antibiotic residues may persist in the distillers grains coproducts. A study to evaluate the fate of virginiamycin during the ethanol production process was conducted in the pilot plant facilities at the National Corn to Ethanol Research Center, Edwardsville, IL. Three 15,000-liter fermentor runs were performed: one with no antibiotic (F1), one dosed with 2 parts per million (ppm) of a commercial virginiamycin product (F2), and one dosed at 20 ppm of virginiamycin product (F3). Fermentor samples, distillers dried grains with solubles (DDGS), and process intermediates (whole stillage, thin stillage, syrup, and wet cake) were collected from each run and analyzed for virginiamycin M and virginiamycin S using a liquid chromatography-mass spectrometry method. Virginiamycin M was detected in all process intermediates of the F3 run. On a dry-weight basis, virginiamycin M concentrations decreased approximately 97 %, from 41 mu g/g in the fermentor to 1.4 mu g/g in the DDGS. Using a disc plate bioassay, antibiotic activity was detected in DDGS from both the F2 and F3 runs, with values of 0.69 mu g virginiamycin equivalent/g sample and 8.9 mu g/g, respectively. No antibiotic activity (<0.6 mu g/g) was detected in any of the F1 samples or in the fermentor and process intermediate samples from the F2 run. These results demonstrate that low concentrations of biologically active antibiotic may persist in distillers grains coproducts produced from fermentations treated with virginiamycin. C1 [Bischoff, Kenneth M.; Rich, Joseph O.] ARS, USDA, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Zhang, Yanhong] So Illinois Univ, Natl Corn Ethanol Res Ctr, Edwardsville, IL 62025 USA. RP Bischoff, KM (reprint author), ARS, USDA, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM kenneth.bischoff@ars.usda.gov NR 14 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 EI 1573-0972 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD MAY PY 2016 VL 32 IS 5 AR 76 DI 10.1007/s11274-016-2026-3 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DK7FD UT WOS:000375089400005 PM 27038946 ER PT J AU Dellape, PM Melo, MC Henry, TJ AF Dellape, Pablo M. Melo, Maria C. Henry, Thomas J. TI A phylogenetic revision of the true bug genus Heraeus (Hemiptera: Rhyparochromidae: Myodochini), with the description of two new genera and 30 new species SO ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY LA English DT Article DE 32 new taxa; cladistics; Heteroptera; Rhyparochrominae; systematics ID HETEROPTERA RHYPAROCHROMIDAE; GALAPAGOS-ISLANDS; LYGAEIDAE; ARGENTINA; LYGAEOIDEA; STAL; REDESCRIPTION; BRAILOVSKY; ECUADOR; RECORDS AB Prior to this study, the genus Heraeus Stal, 1862 included 14 species, all of which are restricted to the Western Hemisphere. Three species are known from the Nearctic Region, nine from the Neotropical Region, and two mainly tropical elements are distributed in both regions. In this contribution, we consider Heraeus cincticornis Stal, 1874 a junior synonym of Heraeus elegans (Walker, 1873), select a lectotype for Heraeus coquilletti Barber, 1914, and neotype for Lygaeus triguttatus Guerin-Meneville, 1857, and describe 28 new species. In addition, the two new genera, Baranowskiobius gen. nov., to include H. elegans (Baranowskiobius elegans comb. nov.) and two new species, and Paraheraeusgen. nov., to include Heraeus eximius Distant, 1882 (Paraheraeus eximius comb. nov.), are described. Previously described species and new taxa are (re)described and illustrated, including male genitalia. Scanning electron micrographs, general habitus photographs, and distribution maps are included for all species studied. A phylogenetic analysis comprising 46 terminal taxa and 50 morphological characters was performed, and five species groups were hypothesized, including the coquilletti, caliginosus, guttatus, illitus, and plebejus groups. All known species of Heraeus and the new genera are included in the phylogenetic analysis. The type species of the genera Myodocha Latreille, 1807, Orthaea Dallas, 1852, and Paisana Dellape, 2008 are used as out-groups. (C) 2016 The Linnean Society of London, Zoological Journal of the Linnean Society, 2016 C1 [Dellape, Pablo M.; Melo, Maria C.] Univ Nacl La Plata, Fac Ciencias Nat & Museo, Div Entomol, Paseo Bosque S-N,B1900FWA, RA-1900 La Plata, Buenos Aires, Argentina. [Dellape, Pablo M.; Melo, Maria C.] Consejo Nacl Invest Cient & Tecn, Cct La Plata, Argentina. [Henry, Thomas J.] Smithsonian Inst, Natl Museum Nat Hist, ARS, Systemat Entomol Lab,USDA, Washington, DC 20560 USA. RP Dellape, PM (reprint author), Univ Nacl La Plata, Fac Ciencias Nat & Museo, Div Entomol, Paseo Bosque S-N,B1900FWA, RA-1900 La Plata, Buenos Aires, Argentina.; Dellape, PM (reprint author), Consejo Nacl Invest Cient & Tecn, Cct La Plata, Argentina. EM pdellape@fcnym.unlp.edu.ar OI Dellape, Pablo M./0000-0002-6914-1026 FU Consejo Nacional de Investigaciones Cientificas y Tecnicas- CONICET, Argentina FX We are grateful to the following people for kindly lending the specimens used in this study: Randall T. Schuh (AMNH); Mick Webb (BMNH); Keve J. Ribardo (CAS); Diego L. Carpintero (MACN); Michael D. Schwartz (CNC); E. Richard Hoebeke (CUIC); Jerome Constant (IRSN); Axel O. Bachmann (MACN); Carlos Campaner (MZSP); Norman F. Johnson (OSUC); Jane O'Donnell (UCMS); John Grehan (PSUC); Robert Brooks (SEMC); and Mark O'Brien (UMMZ). We also thank R.T.Schuh (AMNH), Ruth Salas (AMNH), and Susan Halbert (FSCA) for their help in looking for the holotype of H.concolor, and Eric Guilbert (MNHN) for help looking for the syntypes of H.triguttatus. This work was funded in part by the Consejo Nacional de Investigaciones Cientificas y Tecnicas- CONICET, Argentina. NR 118 TC 0 Z9 0 U1 4 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-4082 EI 1096-3642 J9 ZOOL J LINN SOC-LOND JI Zool. J. Linn. Soc. PD MAY PY 2016 VL 177 IS 1 BP 29 EP 134 DI 10.1111/zoj.12362 PG 106 WC Zoology SC Zoology GA DK7KY UT WOS:000375105100002 ER PT J AU Marek, G Gowda, P Marek, T Auvermann, B Evett, S Colaizzi, P Brauer, D AF Marek, Gary Gowda, Prasanna Marek, Thomas Auvermann, Brent Evett, Steven Colaizzi, Paul Brauer, David TI Estimating preseason irrigation losses by characterizing evaporation of effective precipitation under bare soil conditions using large weighing lysimeters SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Effective precipitation; Irrigation; Evaporation; Semi-arid regions; Weighing lysimeters ID VARIABLE-RATE IRRIGATION; COTTON AB Irrigation from the Ogallala aquifer is used to supplement insufficient precipitation for agricultural crop production in the semi-arid Texas High Plains. Decreased pumping capacity has compelled many producers to "pre-water" fields to field capacity prior to planting to hedge against pumping limitations later in the season. However, the direct measurement of evaporative losses from preseason irrigation of bare soil is not commonly studied. The quantification of evaporative losses from effective precipitation, or the net amount of water that infiltrates into the soil following a precipitation event, can be used as a surrogate for estimating losses from preseason irrigation. We identified 35 precipitation events that occurred over lysimeter fields under fallow conditions in 2002, 2005, and 2009. Events were categorized into four bins of precipitation magnitude ranging from 3 mm to 35 mm. Subsequent evaporation was measured for a period of up to seven days following rainfall events using large weighing lysimeters at the USDA-ARS Conservation and Production Research Laboratory in Bushland, TX. An exponential decay function was used to characterize bare soil evaporation using maximum cumulative measured evaporation (E-Cmax), soil water transfer constant (k), and cumulative grass reference evapotranspiration (ETCos). The wide range of E-Cmax values and k values demonstrated the sensitivity of evaporative losses to both antecedent soil water content and evaporative demand. We also present measured average daily evaporation values for a range of evaporative demand regimes for each precipitation bin. From data analyzed in this study, nearly all of the water from precipitation events of 10 mm and less were lost to evaporation within the following day under moderate to high grass reference evapotranspiration (ETos) conditions. Nearly all water from precipitation events between 20 and 30 mm was lost to evaporation between three to four days following the event under similar evaporative demand. The considerable potential evaporative losses from preseason irrigation call into the question the prudence of the preseason irrigation, particularly for regions with limited groundwater resources. Published by Elsevier B.V. C1 [Marek, Gary; Evett, Steven; Colaizzi, Paul; Brauer, David] ARS, USDA, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. [Gowda, Prasanna] ARS, USDA, Forage & Livestock Prod Res Unit, 7207 West Cheyenne St, El Reno, OK 73036 USA. [Marek, Thomas] Texas A&M AgriLife Res, 6500 Amarillo Blvd West, Amarillo, TX 79106 USA. [Auvermann, Brent] Texas A&M AgriLife Res & Extens, 6500 Amarillo Blvd West, Amarillo, TX 79106 USA. RP Marek, G (reprint author), PO Drawer 10,2300 Expt Stn Rd, Bushland, TX 79012 USA. EM gary.marek@ars.usda.gov FU USDA-ARS Ogallala Aquifer Program; USDA-Agricultural Research Service; Kansas State University; Texas AgriLife Research; Texas AgriLife Extension Service; Texas Tech University; West Texas AM University FX We gratefully acknowledge support from the USDA-ARS Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas AgriLife Research, Texas AgriLife Extension Service, Texas Tech University, and West Texas A&M University. Additionally, many Conservation and Production Research Laboratory scientists and technicians contributed to this work and their contributions are gratefully acknowledged. NR 22 TC 1 Z9 1 U1 5 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD MAY PY 2016 VL 169 BP 115 EP 128 DI 10.1016/j.agwat.2016.02.024 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DJ6YQ UT WOS:000374359500012 ER PT J AU Kang, Y Blanco, K Davis, T Wang, Y DeGrandi-Hoffman, G AF Kang, Yun Blanco, Krystal Davis, Talia Wang, Ying DeGrandi-Hoffman, Gloria TI Disease dynamics of honeybees with Varroa destructor as parasite and virus vector SO MATHEMATICAL BIOSCIENCES LA English DT Article DE Allee Effects; Honeybees; Extinction; Virus; Parasite; Colony Collapse Disorder (CCD) ID COLONY COLLAPSE DISORDER; BEES APIS-MELLIFERA; POPULATION-DYNAMICS; ECTOPARASITIC MITE; MATHEMATICAL-MODEL; JACOBSONI OUD; LIFE-HISTORY; WORKER; HYMENOPTERA; DECLINE AB The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses carried by Varroa mites. In this paper, we propose a honeybee-mite-virus model that incorporates (1) parasitic interactions between honeybees and the Varroa mites; (2) five virus transmission terms between honeybees and mites at different stages of Varroa mites: from honeybees to honeybees, from adult honeybees to the phoretic mites, from brood to the reproductive mites, from the reproductive mites to brood, and from adult honeybees to the phoretic mites; and (3) Allee effects in the honeybee population generated by its internal organization such as division of labor. We provide completed local and global analysis for the full system and its subsystems. Our analytical and numerical results allow us have a better understanding of the synergistic effects of parasitism and virus infections on honeybee population dynamics and its persistence. Interesting findings from our work include: (a) due to Allee effects experienced by the honeybee population, initial conditions are essential for the survival of the colony. (b) Low adult honeybees to brood ratios have destabilizing effects on the system which generate fluctuating dynamics that lead to a catastrophic event where both honeybees and mites suddenly become extinct. This catastrophic event could be potentially linked to Colony Collapse Disorder (CCD) of honeybee colonies. (c) Virus infections may have stabilizing effects on the system, and parasitic mites could make disease more persistent. Our model illustrates how the synergy between the parasitic mites and virus infections consequently generates rich dynamics including multiple attractors where all species can coexist or go extinct depending on initial conditions. Our findings may provide important insights on honeybee viruses and parasites and how to best control them. (C) 2016 Elsevier Inc. All rights reserved. C1 [Kang, Yun] Arizona State Univ, Sci & Math Fac, Coll Letters & Sci, Mesa, AZ 85212 USA. [Blanco, Krystal] Arizona State Univ, Simon A Levin Math & Computat Modeling Sci Ctr, Tempe, AZ 85281 USA. [Davis, Talia] Arizona State Univ, Sch Math & Stat Sci, Tempe, AZ 85281 USA. [Wang, Ying] Arizona State Univ, Sch Life Sci, Tempe, AZ 85281 USA. [DeGrandi-Hoffman, Gloria] USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ 85719 USA. RP Kang, Y (reprint author), Arizona State Univ, Sci & Math Fac, Coll Letters & Sci, Mesa, AZ 85212 USA. EM yun.kang@asu.edu OI Kang, Yun/0000-0001-5448-7609 FU NSF-DMS [1313312] FX Y.K's research is partially supported by NSF-DMS (1313312). We really appreciate useful comments and suggestions provided by two reviewers to significantly improve this manuscript. NR 75 TC 0 Z9 1 U1 34 U2 68 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0025-5564 EI 1879-3134 J9 MATH BIOSCI JI Math. Biosci. PD MAY PY 2016 VL 275 BP 71 EP 92 DI 10.1016/j.mbs.2016.02.012 PG 22 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA DK8HY UT WOS:000375169000008 PM 26968931 ER PT J AU Garcia, MO Smith, JE Luoma, DL Jones, MD AF Garcia, Maria O. Smith, Jane E. Luoma, Daniel L. Jones, Melanie D. TI Ectomycorrhizal communities of ponderosa pine and lodgepole pine in the south-central Oregon pumice zone SO MYCORRHIZA LA English DT Article DE Climate change; Ectomycorrhizal communities; Pine species migration; Pinus contorta; Pinus ponderosa ID YELLOWSTONE-NATIONAL-PARK; FINE-ROOT BIOMASS; MYCORRHIZAL FUNGI; SPECIES RICHNESS; BRITISH-COLUMBIA; HOST-SPECIFICITY; CLIMATE-CHANGE; DOUGLAS-FIR; NITROGEN DEPOSITION; PACIFIC-NORTHWEST AB Forest ecosystems of the Pacific Northwest of the USA are changing as a result of climate change. Specifically, rise of global temperatures, decline of winter precipitation, earlier loss of snowpack, and increased summer drought are altering the range of Pinus contorta. Simultaneously, flux in environmental conditions within the historic P. contorta range may facilitate the encroachment of P. ponderosa into P. contorta territory. Furthermore, successful pine species migration may be constrained by the distribution or co-migration of ectomycorrhizal fungi (EMF). Knowledge of the linkages among soil fungal diversity, community structure, and environmental factors is critical to understanding the organization and stability of pine ecosystems. The objectives of this study were to establish a foundational knowledge of the EMF communities of P. ponderosa and P. contorta in the Deschutes National Forest, OR, USA, and to examine soil characteristics associated with community composition. We examined EMF root tips of P. ponderosa and P. contorta in soil cores and conducted soil chemistry analysis for P. ponderosa cores. Results indicate that Cenococcum geophilum, Rhizopogon salebrosus, and Inocybe flocculosa were dominant in both P. contorta and P. ponderosa soil cores. Rhizopogon spp. were ubiquitous in P. ponderosa cores. There was no significant difference in the species composition of EMF communities of P. ponderosa and P. contorta. Ordination analysis of P. ponderosa soils suggested that soil pH, plant-available phosphorus (Bray), total phosphorus (P), carbon (C), mineralizable nitrogen (N), ammonium (NH4), and nitrate (NO3) are driving EMF community composition in P. ponderosa stands. We found a significant linear relationship between EMF species richness and mineralizable N. In conclusion, P. ponderosa and P. contorta, within the Deschutes National Forest, share the same dominant EMF species, which implies that P. ponderosa may be able to successfully establish within the historic P. contorta range and dominant EMF assemblages may be conserved. C1 [Garcia, Maria O.; Luoma, Daniel L.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Garcia, Maria O.] Boston Univ, Dept Biol, 5 Cummington Mall, Boston, MA 02215 USA. [Smith, Jane E.] US DOE, Forest Serv, Pacific NW Res Stn, Forestry Sci Lab, 3200 JeffersonWay, Corvallis, OR 97331 USA. [Jones, Melanie D.] Univ British Columbia, Inst Biodivers Resilience & Ecosyst Serv, Dept Biol, Okanagan Campus, Kelowna, BC V1V 1V7, Canada. RP Smith, JE (reprint author), US DOE, Forest Serv, Pacific NW Res Stn, Forestry Sci Lab, 3200 JeffersonWay, Corvallis, OR 97331 USA. EM jsmith01@fs.fed.us FU National Science Foundation Graduate Research Fellowship Program; USDA Forest Service Pacific Northwest Research Station FX This research was made possible with funding received from the National Science Foundation Graduate Research Fellowship Program and the USDA Forest Service Pacific Northwest Research Station. Mention of trade or firm names does not constitute an endorsement by the US Department of Agriculture. We thank Levi Davis, Tara Jennings, Doni McKay, and Joyce Eberhart for the lab assistance and Norman Forsberg for the field assistance. Thanks to Mary Berbee for the help with data analysis, Ed Mitchell and Bruce McCune for the help with the statistical analysis, and Kelly Christiansen for the GIS assistance. The comments of Greg Brenner, Paul Doescher, Marty Kranabetter, Pablo Martin-Pinto, and two anonymous reviewers greatly improved this manuscript. NR 82 TC 1 Z9 1 U1 6 U2 28 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0940-6360 EI 1432-1890 J9 MYCORRHIZA JI Mycorrhiza PD MAY PY 2016 VL 26 IS 4 BP 275 EP 286 DI 10.1007/s00572-015-0668-x PG 12 WC Mycology SC Mycology GA DJ7PN UT WOS:000374403400002 PM 26547440 ER PT J AU Vannella, KM Ramalingam, TR Hart, KM Prado, RD Sciurba, J Barron, L Borthwick, LA Smith, AD Mentink-Kane, M White, S Thompson, RW Cheever, AW Bock, K Moore, I Fitz, LJ Urban, JF Wynn, TA AF Vannella, Kevin M. Ramalingam, Thirumalai R. Hart, Kevin M. Prado, Rafael de Queiroz Sciurba, Joshua Barron, Luke Borthwick, Lee A. Smith, Allen D. Mentink-Kane, Margaret White, Sandra Thompson, Robert W. Cheever, Allen W. Bock, Kevin Moore, Ian Fitz, Lori J. Urban, Joseph F., Jr. Wynn, Thomas A. TI Acidic chitinase primes the protective immune response to gastrointestinal nematodes SO NATURE IMMUNOLOGY LA English DT Article ID CD103(+) DENDRITIC CELLS; GENE-EXPRESSION PROFILES; MAMMALIAN CHITINASE; NIPPOSTRONGYLUS-BRASILIENSIS; LYMPHOID-CELLS; CHITOTRIOSIDASE; DISTINCT; TISSUE; ROLES; IL-13 AB Acidic mammalian chitinase (AMCase) is known to be induced by allergens and helminths, yet its role in immunity is unclear. Using AMCase-deficient mice, we show that AMCase deficiency reduced the number of group 2 innate lymphoid cells during allergen challenge but was not required for establishment of type 2 inflammation in the lung in response to allergens or helminths. In contrast, AMCase-deficient mice showed a profound defect in type 2 immunity following infection with the chitin-containing gastrointestinal nematodes Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. The impaired immunity was associated with reduced mucus production and decreased intestinal expression of the signature type 2 response genes Il13, Chil3, Retnlb, and Clca1. CD103(+) dendritic cells, which regulate T cell homing, were also reduced in mesenteric lymph nodes of infected AMCase-deficient mice. Thus, AMCase functions as a critical initiator of protective type 2 responses to intestinal nematodes but is largely dispensable for allergic responses in the lung. C1 [Vannella, Kevin M.; Ramalingam, Thirumalai R.; Hart, Kevin M.; Prado, Rafael de Queiroz; Sciurba, Joshua; Barron, Luke; Borthwick, Lee A.; Mentink-Kane, Margaret; White, Sandra; Thompson, Robert W.; Cheever, Allen W.; Wynn, Thomas A.] NIAID, Program Tissue Immun & Repair, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. [Borthwick, Lee A.] Newcastle Univ, Inst Cellular Med, Tissue Fibrosis & Repair Grp, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Smith, Allen D.; Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Ctr, Beltsville, MD USA. [Bock, Kevin; Moore, Ian] NIAID, Infect Dis Pathogenesis Sect, NIH, Rockville, MD USA. [Fitz, Lori J.] Pfizer Worldwide R&D, Inflammat & Immun, Cambridge, MA USA. RP Wynn, TA (reprint author), NIAID, Program Tissue Immun & Repair, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. EM twynn@niaid.nih.gov FU Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Disease FX This research was supported by the Intramural Research Program of the National Institutes of Health, National Institute of Allergy and Infectious Disease. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank MedImmune for generating the anti-AMCase rabbit sera, C. Mainhart for genotyping, T. Gieseck and K. Kindrachuk for discussions, and the animal care staffs of Buildings 50 and 14BS at the US National Institutes of Health's Bethesda, Maryland campus for the conscientious care of mice. NR 32 TC 3 Z9 4 U1 8 U2 15 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1529-2908 EI 1529-2916 J9 NAT IMMUNOL JI Nat. Immunol. PD MAY PY 2016 VL 17 IS 5 BP 538 EP + DI 10.1038/ni.3417 PG 9 WC Immunology SC Immunology GA DJ6LA UT WOS:000374323100013 PM 27043413 ER PT J AU Reinhart, KO Rinella, MJ AF Reinhart, Kurt O. Rinella, Matthew J. TI A common soil handling technique can generate incorrect estimates of soil biota effects on plants SO NEW PHYTOLOGIST LA English DT Article DE microbial ecology; plant-soil biota experiment; plant-soil feedback; soil handling technique; type I error ID PRUNUS-SEROTINA; TREE; FEEDBACKS; INVASION; DISEASE; SUCCESSION; PATHOGENS; DYNAMICS; ECOLOGY; GROWTH C1 [Reinhart, Kurt O.; Rinella, Matthew J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. RP Reinhart, KO (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM kurt.reinhart@ars.usda.gov NR 20 TC 7 Z9 7 U1 6 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD MAY PY 2016 VL 210 IS 3 BP 786 EP 789 DI 10.1111/nph.13822 PG 4 WC Plant Sciences SC Plant Sciences GA DJ5YM UT WOS:000374286700005 PM 26738893 ER PT J AU Norman, LM Brinkerhoff, F Gwilliam, E Guertin, DP Callegary, J Goodrich, DC Nagler, PL Gray, F AF Norman, L. M. Brinkerhoff, F. Gwilliam, E. Guertin, D. P. Callegary, J. Goodrich, D. C. Nagler, P. L. Gray, F. TI HYDROLOGIC RESPONSE OF STREAMS RESTORED WITH CHECK DAMS IN THE CHIRICAHUA MOUNTAINS, ARIZONA SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE check dams; restoration; semi-arid watersheds; water budget; runoff ratio ID TURKEY CREEK CALDERA; SEMIARID WATERSHEDS; EROSION CONTROL; RUNOFF; SOIL; VARIABILITY; CATCHMENT; RANGELAND; SOUTHWEST; YIELD AB In this study, hydrological processes are evaluated to determine impacts of stream restoration in the West Turkey Creek, Chiricahua Mountains, southeast Arizona, during a summer-monsoon season (June-October of 2013). A paired-watershed approach was used to analyze the effectiveness of check dams to mitigate high flows and impact long-term maintenance of hydrologic function. One watershed had been extensively altered by the installation of numerous small check dams over the past 30years, and the other was untreated (control). We modified and installed a new stream-gauging mechanism developed for remote areas, to compare the water balance and calculate rainfall-runoff ratios. Results show that even 30years after installation, most of the check dams were still functional. The watershed treated with check dams has a lower runoff response to precipitation compared with the untreated, most notably in measurements of peak flow. Concerns that downstream flows would be reduced in the treated watershed, due to storage of water behind upstream check dams, were not realized; instead, flow volumes were actually higher overall in the treated stream, even though peak flows were dampened. We surmise that check dams are a useful management tool for reducing flow velocities associated with erosion and degradation and posit they can increase baseflow in aridlands. (C) 2015 The Authors. River Research and Applications published by John Wiley & Sons, Ltd. C1 [Norman, L. M.] US Geol Survey, Western Geog Sci Ctr, 520N Pk Ave,Ste 102K, Tucson, AZ 85719 USA. [Brinkerhoff, F.; Callegary, J.] US Geol Survey, Arizona Water Sci Ctr, 520N Pk Ave,Ste 102K, Tucson, AZ 85719 USA. [Gwilliam, E.] Natl Pk Serv, Sonoran Desert Network, Tucson, AZ USA. [Guertin, D. P.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Goodrich, D. C.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. [Nagler, P. L.] US Geol Survey, Southwest Biol Sci Ctr, Sonoran Desert Res Stn, 520N Pk Ave,Ste 102K, Tucson, AZ 85719 USA. [Gray, F.] US Geol Survey, Geol Minerals Energy & Geophys Sci Ctr, 520N Pk Ave,Ste 102K, Tucson, AZ 85719 USA. RP Norman, LM (reprint author), US Geol Survey, Western Geog Sci Ctr, 520N Pk Ave,Ste 102K, Tucson, AZ 85719 USA. EM lnorman@usgs.gov FU Land Change Science (LCS) Program, under the Climate and Land Use Change (CLU) Mission Area of the U.S. Geological Survey (USGS) FX This research was conducted with support from the Land Change Science (LCS) Program, under the Climate and Land Use Change (CLU) Mission Area of the U.S. Geological Survey (USGS). The National Park Service's Sonoran Desert Network (NPS SODN) supplied equipment for the study in relationship to nearby Chiricahua National Monument. We appreciate the peer reviews done by Stephen Wiele and Ann Youberg. Many thanks to Valer and Josiah Austin (EC; Cuenca Los Ojos Foundation); Joel Sankey, Miguel Villarreal, Leila Gass, Jeff Cordova and Hanna Coy (USGS); Jon Pelletier and Edward Glenn (University of Arizona); Kara Raymond (NPS); Erin Boyle (National Weather Service); Michele Girard and Duane Bennett (USFS, Coronado National Forest); Ryan Faught and Chelsea Smith (University of Oklahoma); Ron Pulliam and David Seibert (Borderlands Restoration); and Gerry Norman for all their help making our research a success. References to commercial vendors of software products or services are provided solely for the convenience of users when obtaining or using USGS software. Such references do not imply any endorsement by the U.S. Government. NR 63 TC 1 Z9 1 U1 2 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1535-1459 EI 1535-1467 J9 RIVER RES APPL JI River Res. Appl. PD MAY PY 2016 VL 32 IS 4 BP 519 EP 527 DI 10.1002/rra.2895 PG 9 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA DK1WE UT WOS:000374704900001 ER PT J AU Mendoza, JA Baranowski, T Jaramillo, S Fesinmeyer, MD Haaland, W Thompson, D Nicklas, TA AF Mendoza, Jason A. Baranowski, Tom Jaramillo, Sandra Fesinmeyer, Megan D. Haaland, Wren Thompson, Debbe Nicklas, Theresa A. TI Fit 5 Kids TV Reduction Program for Latino Preschoolers A Cluster Randomized Controlled Trial SO AMERICAN JOURNAL OF PREVENTIVE MEDICINE LA English DT Article ID YOUNG-CHILDREN; PHYSICAL-ACTIVITY; UNITED-STATES; BODY-MASS; TELEVISION; OBESITY; HEALTH; ACCELEROMETER; ADOLESCENTS; MEDIA AB Introduction: Reducing Latino preschoolers' TV viewing is needed to reduce their risk of obesity and other chronic diseases. This study's objective was to evaluate the Fit 5 Kids (F5K) TV reduction program's impact on Latino preschooler's TV viewing. Study design: Cluster RCT with randomization at the center level and N=160 participants. Setting/participants: Latino children aged 3-5 years and their parents were recruited from six Head Start centers in Houston TX in 2010-2012 with analyses in 2013-2014. Intervention: F5K was culturally adapted for Latino preschoolers and the overall goal was to reduce TV viewing. Study staff taught F5K over 7-8 weeks during the regular Head Start day directly to intervention students. Control schools provided the usual Head Start curriculum, which did not specifically cover TV viewing. Main outcome measures: Individual-level outcomes were measured prior to (Time 1) and immediately following (Time 2) the intervention. The primary outcome, TV viewing (minutes/day), was measured by validated 7-day TV diaries (parent-reported). Sedentary time was measured by accelerometers. Results: Per the adjusted repeated measures linear mixed effects model for TV viewing (minutes/day), intervention children decreased from 76.2 (9.9) at Time 1 to 52.1 (10.0) at Time 2, whereas control children remained about the same from 84.2 (10.5) at Time 1 to 85.4 (10.5) at Time 2. The relative difference from Time 1 to Time 2 was -25.3 (95% CI= -45.2, -5.4) minutes for intervention versus control children (N=160, p=0.01). In a similar adjusted model, there was a relative decrease in sedentary time (minutes/day) from Time 1 to Time 2 favoring the intervention children (-9.5, 95% CI= -23.0, 4.1), although not significant at p < 0.05. Conclusions: F5K reduced Latino preschoolers' TV viewing by >25 minutes daily. These findings have implications for prevention of obesity, related disorders, and health equity. (C) 2016 American Journal of Preventive Medicine. All rights reserved. C1 [Mendoza, Jason A.] Univ Washington, Sch Med, Dept Pediat, Gen Pediat, Seattle, WA 98195 USA. [Mendoza, Jason A.] Univ Washington, Sch Publ Hlth, Nutr Sci Program, Seattle, WA 98195 USA. [Mendoza, Jason A.; Fesinmeyer, Megan D.; Haaland, Wren] Seattle Childrens Res Inst, Ctr Child Hlth Behav & Dev, Seattle, WA USA. [Mendoza, Jason A.] Fred Hutchinson Canc Res Ctr, Hlth Dispar Res Ctr, 1124 Columbia St, Seattle, WA 98104 USA. [Baranowski, Tom; Jaramillo, Sandra; Thompson, Debbe; Nicklas, Theresa A.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Mendoza, JA (reprint author), POB 5371,M-S CW8-6,Suite 400, Seattle, WA 98145 USA. EM jason.mendoza@seattlechildrens.org FU National Cancer Institute of the NIH [K07CA131178]; U.S. Department of Agriculture [6250-51000-053] FX The investigators are grateful to the preschoolers and parents who participated in the study, as well as the Head Start centers and administrators from AVANCE and Neighborhood Centers, Inc., who graciously provided input and allowed the team to conduct this study. The investigative team acknowledges the kind encouragement and support of Dr. Barbara Dennison, who previously developed and tested the F5K curriculum with colleagues and who agreed to its cultural adaptation and testing in the present study. The content of this report is solely the responsibility of the authors and does not necessarily represent the official views of the funders or the authors' affiliated academic institutions. Research reported in this publication was supported by a career development award to the first author while at the Children's Nutrition Research Center of Baylor College of Medicine from the National Cancer Institute of the NIH under award number K07CA131178 and the U.S. Department of Agriculture Cooperative Agreement number 6250-51000-053. The funders had no role in the design, collection, analysis, and interpretation of data or writing/submission of this report. This study was approved by the IRBs of Baylor College of Medicine (July 2009) and Seattle Children's Research Institute (August 2013). NR 45 TC 0 Z9 0 U1 2 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0749-3797 EI 1873-2607 J9 AM J PREV MED JI Am. J. Prev. Med. PD MAY PY 2016 VL 50 IS 5 BP 584 EP 592 DI 10.1016/j.amepre.2015.09.017 PG 9 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA DJ5VJ UT WOS:000374277900004 PM 26572093 ER PT J AU Jennings, VL Larson, CK Larson, LR AF Jennings, Viniece L. Larson, Claire K. Larson, Lincoln R. TI Ecosystem Services and Preventive Medicine A Natural Connection SO AMERICAN JOURNAL OF PREVENTIVE MEDICINE LA English DT Article ID PHYSICAL-ACTIVITY; HUMAN HEALTH; ENVIRONMENTAL-HEALTH; BENEFITS; GREEN C1 [Jennings, Viniece L.] US Forest Serv, USDA, Athens, GA USA. [Larson, Claire K.] Univ Calif San Francisco, Dept Med, San Francisco, CA USA. [Larson, Lincoln R.] Clemson Univ, Dept Pk Recreat & Tourism Management, Clemson, SC USA. RP Jennings, VL (reprint author), US Forest Serv, USDA, Southern Res Stn, Integrating Human & Nat Syst, 320 Green St, Athens, GA 30602 USA. EM vjennings02@fs.fed.us FU U.S. Department of Agriculture Forest Service [JV-11330144-060] FX This project is supported by an agreement (JV-11330144-060) funded by the U.S. Department of Agriculture Forest Service. The authors would also like to thank Dr. Myron Floyd, whose review advanced the concepts expressed in this manuscript. NR 23 TC 2 Z9 2 U1 2 U2 8 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0749-3797 EI 1873-2607 J9 AM J PREV MED JI Am. J. Prev. Med. PD MAY PY 2016 VL 50 IS 5 BP 642 EP 645 DI 10.1016/j.amepre.2015.11.001 PG 4 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA DJ5VJ UT WOS:000374277900011 PM 26699248 ER PT J AU Cui, Z Ojaghian, MR Tao, Z Kakar, KU Zeng, J Zhao, W Duan, Y Cruz, CMV Li, B Zhu, B Xie, G AF Cui, Z. Ojaghian, M. R. Tao, Z. Kakar, K. U. Zeng, J. Zhao, W. Duan, Y. Vera Cruz, C. M. Li, B. Zhu, B. Xie, G. TI Multiplex PCR assay for simultaneous detection of six major bacterial pathogens of rice SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE comparative genomics; detection; multiplex PCR; quantitative real-time PCR; rice bacterial pathogens ID ORYZAE PV. ORYZICOLA; POLYMERASE-CHAIN-REACTION; MEDIATED ISOTHERMAL AMPLIFICATION; COMPLETE GENOME SEQUENCE; SOUTHERN UNITED-STATES; AVENAE SUBSP AVENAE; PSEUDOMONAS-FUSCOVAGINAE; BURKHOLDERIA-GLUMAE; PANICLE BLIGHT; SENSITIVE DETECTION AB AimsThe aim of this study was to develop a multiplex PCR (mPCR) assay for rapid, sensitive and simultaneous detection of six important rice pathogens: Xanthomonas oryzae pv. oryzae, X.oryzae pv. oryzicola, Pseudomonas fuscovaginae, Burkholderia glumae, Burkholderia gladioli and Acidovorax avenae subsp.avenae. Methods and ResultsSpecific primers were designed through a bioinformatics pipeline. Sensitivity of detection was established using both traditional PCR and quantitative real-time PCR on isolated DNA and on bacterial cells both invitro and in simulated diseased seeds and the parameters were optimized for an mPCR assay. A total of 150 bacterial strains were tested for specificity. The mPCR assay accurately predicted the presence of pathogens among 44 symptomatic and asymptomatic rice seed, sheath and leaf samples. ConclusionsThis study confirmed that this mPCR assay is a rapid, reliable and simple tool for the simultaneous detection of six important rice bacterial pathogens. Significance and Impact of the StudyThis study is the first report of a method allowing simultaneous detection of six major rice pathogens. The ability to use crude extracts from plants without bacterial isolation or DNA extraction enhances the value of this mPCR technology for rapid detection and aetiological/epidemiological studies. C1 [Cui, Z.; Ojaghian, M. R.; Tao, Z.; Kakar, K. U.; Zeng, J.; Li, B.; Zhu, B.; Xie, G.] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China. [Zhao, W.] Chinese Acad Inspect & Quarantine, Beijing, Peoples R China. [Duan, Y.] USDA ARS USHRL, Ft Pierce, FL USA. [Vera Cruz, C. M.] Int Rice Res Inst, Plant Breeding Genet & Biotechnol Div, Manila, Philippines. [Zhu, B.] Zhejiang Sci Tech Univ, Coll Life Sci, Zhejiang Prov Key Lab Plant Secondary Metab & Reg, Hangzhou, Zhejiang, Peoples R China. RP Zhu, B; Xie, G (reprint author), Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Zhejiang, Peoples R China. EM bzhu@zstu.edu.cn; glxie@zju.edu.cn FU Special Fund for Agro-Scientific Research in the Public Interest [201303015]; National Natural Science Foundation of China [31200003]; 12th Five Years Key Programs for Science and Technology Development of China [2012BAK11B02, 2012BAK11B06]; Zhejiang Provincial Nature Science Foundation of China [LY12C14007] FX This project was supported by the Special Fund for Agro-Scientific Research in the Public Interest (201303015); National Natural Science Foundation of China (31200003); 12th Five Years Key Programs for Science and Technology Development of China (2012BAK11B02; 2012BAK11B06); Zhejiang Provincial Nature Science Foundation of China (LY12C14007). We thank the BCCM/LMG Bacteria Collection of Belgium for providing the strains used in this study. NR 49 TC 1 Z9 1 U1 9 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAY PY 2016 VL 120 IS 5 BP 1357 EP 1367 DI 10.1111/jam.13094 PG 11 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DJ5NV UT WOS:000374256600019 PM 26864896 ER PT J AU Ebel, ED Williams, MS Golden, NJ Tankson, J AF Ebel, E. D. Williams, M. S. Golden, N. J. Tankson, J. TI Bayesian techniques for comparison of the test performance of PCR and culture for the identification of Campylobacter in enriched comminuted chicken samples SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE BAX((R)) system real-time PCR assay for Campylobacter jejuni; coli; lari; Bayesian; Campylobacter; prevalence; sensitivity; specificity ID LATENT CLASS MODELS; DIAGNOSTIC-TESTS; UNITED-STATES; SCREENING TEST; OUTBREAK DATA; COLI; JEJUNI; SENSITIVITY; PREVALENCE; ATTRIBUTION AB AimsUsing Bayesian methods that do not require the definition of a gold standard, the diagnostic sensitivity and specificity of a real-time polymerase chain reaction (PCR) assay are compared to those of an enriched culture assay for detection of Campylobacter in enriched comminuted chicken samples. Methods and ResultsFood Safety and Inspection Service comminuted chicken samples were collected from production facilities across the United States. Enriched samples were examined using a commercial real-time PCR kit and plated for culture. Allowing for conditional dependence between these approaches and defining relatively uninformed prior distributions, the no gold standard' Bayesian methods generated estimates of the means (95% credible interval) of the posterior distributions for sensitivity and specificity of the PCR as 93% (79, 100%) and 95% (87, 100%) respectively. The estimated sensitivity implies a mean false negative frequency of 7%. The estimated means of the posterior distributions for sensitivity and specificity of the culture assay were 91% (76, 100%) and 96% (88, 100%) respectively. In this case, the mean false negative frequency is 9%. Graphical comparisons of the posterior distributions with their corresponding prior distributions suggested only subtle differences in the sensitivities of both tests, but the posterior distributions for specificities are substantially more certain than the prior distributions. ConclusionsThe study suggests that the commercial real-time PCR assay is a more sensitive screening test that would provide timelier negative test results. The modest 1% reduction in specificity of this PCR assay, as compared to an enriched culture assay, is less of a concern for regulatory testing programs if a culture-based confirmatory assay is applied to all presumptive positive samples. Significance and Impact of the StudyThe sensitivity and specificity of a PCR assay and a culture assay for Campylobacter in comminuted poultry produced in the United States were estimated. The PCR assay was shown to be an appropriate alternative screening test. C1 [Ebel, E. D.; Williams, M. S.; Golden, N. J.; Tankson, J.] USDA, Food Safety Inspect Serv, Off Publ Hlth Sci, Ft Collins, CO USA. RP Williams, MS (reprint author), 2150 Ctr Ave,Bldg D, Ft Collins, CO USA. EM mike.williams@fsis.usda.gov NR 35 TC 0 Z9 0 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAY PY 2016 VL 120 IS 5 BP 1418 EP 1426 DI 10.1111/jam.13098 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DJ5NV UT WOS:000374256600024 PM 26895234 ER PT J AU Shan, L Yang, D Wang, DP Tian, P AF Shan, Lei Yang, David Wang, Dapeng Tian, Peng TI Comparison of cell-based and PCR-based assays as methods for measuring infectivity of Tulane virus SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Tulane virus; Correlation; Plaque assay; TCID50 assay; ISC-qRT-PCR assay; RNase exposure assay; Infectivity; Correlation ID FELINE-CALICIVIRUS; MURINE NOROVIRUS; INACTIVATION AB In this study, we used Tulane virus (TV) as a surrogate for HuNoV to evaluate for correlation between two cell-based assays and three PCR-based assays. Specifically, the cell-based plaque and TCID50 assays measure for infectious virus particles, while the PCR-based RNase exposure, porcine gastric mucin in-situ capture qRT-PCR (PGM-ISC-qRT-PCR), and antibody in-situ-capture qRT-PCR (Ab-ISC-qRT-PCR) assays measure for an amplicon within encapsidated viral genome. Ten batches of viral stocks ranging from 3.41 x 10(5) to 6.67 x 10(6) plaque forming units (PFUs) were used for side by side comparison with PFU as a reference. The results indicate that one PFU was equivalent to 6.69 +/- 2.34 TCID50 units, 9.75 +/- 10.87 RNase-untreated genomic copies (GCs), 2.87 +/- 3.05 RNase-treated GCs, 0.07 +/- 0.07 PGM-ISC-qRT-PCR GCs, and 0.52 +/- 0.39 Ab-ISC-qRT-PCR GCs. We observed that while the cell-based assays were consistent with each other, the TCID50 assay was more sensitive than the plaque assay. In contrast, the PCR-based assays were not always consistent with the cell-based assays. The very high variations in GCs as measured by both ISC-RT-qPCR assays made them difficult to correlate against the relatively small variations (<20 fold) in the PFUs or TCID50 units as measured by the cell-based assays. Published by Elsevier B.V. C1 [Shan, Lei; Yang, David; Tian, Peng] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Wang, Dapeng] Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety & Bor Luh Foo, Shanghai 200240, Peoples R China. RP Tian, P (reprint author), ARS, WRRC, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM peng.tian@ars.usda.gov FU USDA Agricultural Research Service CRIS project [5325-42000-046-06R] FX This work was supported by USDA Agricultural Research Service CRIS project 5325-42000-046-06R. USDA is an equal opportunity provider and employer. NR 18 TC 1 Z9 1 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD MAY PY 2016 VL 231 BP 1 EP 7 DI 10.1016/j.jviromet.2016.01.012 PG 7 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA DJ4TH UT WOS:000374200400001 PM 26875997 ER PT J AU Lawrence, P Rai, D Conderino, JS Uddowla, S Rieder, E AF Lawrence, Paul Rai, Devendra Conderino, Joseph S. Uddowla, Sabena Rieder, Elizabeth TI Role of Jumonji C-domain containing protein 6 (JMJD6) in infectivity of CD foot-and-mouth disease virus SO VIROLOGY LA English DT Article DE Jumonji C-domain containing ptotein 6 (JMJD6); Foot-and-mouth disease virus (FMDV); FMDV alternate receptor ID APOPTOTIC CELLS; PHOSPHATIDYLSERINE RECEPTOR; HEPARAN-SULFATE; CULTURE ADAPTATION; GENE-PRODUCT; PREDICTION; TOPOLOGY; BINDING; SURFACE; PATHOGENESIS AB Foot-and-mouth disease virus (FMDV) utilizes four integrins (alpha(v)beta(1),alpha(v)beta(3),alpha(v)beta(6), and alpha(v)beta(8)) as its primary cell receptor. During cell culture propagation, FMDV frequently adapts to use heparan sulfate (HS), and rarely utilizes an unidentified third receptor. Capsid mutations acquired by a soluble integrin resistant FMDV cause (i) adaptation to CHO-677 cells (ii) increased affinity to membrane-bound Jumonji C-domain containing protein 6 (JMJD6) (iii) induced JMJD6 re-localization from the cell surface and cytoplasm to the nucleus. Interestingly, pre-treatment of cells with N- and C-terminal JMJD6 antibodies or by simultaneous incubation of mutant virus with soluble JMJD6 (but not by treatment with HS or alpha(v)beta(6)) impaired virus infectivity in cultured cells. JMJD6 and mutant virus co-purified by reciprocal co-immunoprecipitation. Molecular docking predictions suggested JMJD6 C-terminus interacts with mutated VP1 capsid protein. We conclude when specific VP1 mutations are displayed, JMJD6 contributes to FMDV infectivity and may be a previously unidentified FMDV receptor. Published by Elsevier Inc. C1 [Lawrence, Paul; Rai, Devendra; Conderino, Joseph S.; Uddowla, Sabena; Rieder, Elizabeth] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Uddowla, Sabena] Univ Cent Florida, Environm Hlth & Safety, 3512 Perseus Loop,Bldg 48, Orlando, FL 32816 USA. RP Rieder, E (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, NAA, POB 848, Greenport, NY 11944 USA. EM Elizabeth.Rieder@ars.usda.gov FU United States Department of Agriculture FX Funding for the research detailed in this manuscript was provided through Congressionally allocated dollars for the Agricultural Research Service of the United States Department of Agriculture. NR 70 TC 1 Z9 1 U1 1 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAY PY 2016 VL 492 BP 38 EP 52 DI 10.1016/j.virol.2016.02.005 PG 15 WC Virology SC Virology GA DJ4WX UT WOS:000374209900006 PM 26896934 ER PT J AU Bartels, M French, R Graybosch, RA Tatineni, S AF Bartels, Melissa French, Roy Graybosch, Robert A. Tatineni, Satyanarayana TI Triticum mosaic virus exhibits limited population variation yet shows evidence of parallel evolution after replicated serial passage in wheat SO VIROLOGY LA English DT Article DE Wheat virus; Triticum mosaic virus; Potyviridae; Genetic variation; Evolution; Population genetics ID STRAND-CONFORMATION POLYMORPHISM; RETROVIRAL REVERSE TRANSCRIPTASES; PLANT RNA VIRUS; GENETIC DIVERSITY; MUTATION-RATES; FAMILY POTYVIRIDAE; GREAT-PLAINS; NATURAL-POPULATIONS; TEMPORAL DYNAMICS; SYSTEMIC MOVEMENT AB An infectious cDNA clone of Triticum mosaic virus (TriMV) (genus Poacevirus; family Potyviridae) was used to establish three independent lineages in wheat to examine intra-host population diversity levels within protein 1 (P1) and coat protein (CP) cistrons over time. Genetic variation was assessed at passages 9, 18 and 24 by single-strand conformation polymorphism, followed by nucleotide sequencing. The founding P1 region genotype was retained at high frequencies in most lineage/passage populations, while the founding CP genotype disappeared after passage 18 in two lineages. We found that rare TriMV genotypes were present only transiently and lineages followed independent evolutionary trajectories, suggesting that genetic drift dominates TriMV evolution. These results further suggest that experimental populations of TriMV exhibit lower mutant frequencies than that of Wheat streak mosaic virus (genus Tritimovirus; family Potyviridae) in wheat. Nevertheless, there was evidence for parallel evolution at a synonymous site in the TriMV CP cistron. Published by Elsevier Inc. C1 [Bartels, Melissa; French, Roy; Graybosch, Robert A.; Tatineni, Satyanarayana] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Bartels, Melissa; French, Roy; Tatineni, Satyanarayana] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Graybosch, Robert A.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Bartels, Melissa] Syngenta, 6338 Highway 20-26, Nampa, ID 83687 USA. RP French, R; Tatineni, S (reprint author), Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. EM roy_french@mac.com NR 67 TC 1 Z9 1 U1 3 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAY PY 2016 VL 492 BP 92 EP 100 DI 10.1016/j.virol.2016.02.010 PG 9 WC Virology SC Virology GA DJ4WX UT WOS:000374209900011 PM 26914507 ER PT J AU Lawrence, P Pacheco, J Stenfeldt, C Arzt, J Rai, DK Rieder, E AF Lawrence, Paul Pacheco, Juan Stenfeldt, Carolina Arzt, Jonathan Rai, Devendra K. Rieder, Elizabeth TI Pathogenesis and micro-anatomic characterization of a cell-adapted mutant foot-and-mouth disease virus in cattle: Impact of the Jumonji C-domain containing protein 6 (JMJD6) and route of inoculation SO VIROLOGY LA English DT Article DE Jumonji C-domain containing protein 6 (JMJD6); Foot-and-mouth disease virus (FMDV); FMDV third receptor; Viral pathogenesis ID PHOSPHATIDYLSERINE RECEPTOR; APOPTOTIC CELLS; HEPARAN-SULFATE; CULTURE ADAPTATION; GENE-PRODUCT; BINDING SITE; IN-VITRO; INFECTION; CLEARANCE; SURFACE AB A companion study reported Jumonji-C domain containing protein 6 (JMJD6) is involved in an integrin- and HS-independent pathway of FMDV infection in CHO cells. JMJD6 localization was investigated in animal tissues from cattle infected with either wild type A24-FMDV (A24-WT) or mutant FMDV (JMJD6-FMDV) carrying E95K/S96L and RGD to KGE mutations in VP1. Additionally, pathogenesis of mutant JMJD6-FMDV was investigated in cattle through aerosol and intraepithelial lingual (IEL) inoculation. Interestingly, JMJD6-FMDV pathogenesis was equivalent to A24-WT administered by IEL route. In contrast, JMJD6-FMDV aerosol-infected cattle did not manifest signs of FMD and animals showed no detectable viremia. Immunofluorescent microscopy of post-mortem tissue revealed JMJD6-FMDV exclusively co-localized with JMJD6(+) cells while A24-WT was occasionally found in JMJD6(+) cells. In vitro, chemical uptake inhibitors demonstrated JMJD6-FMDV entered cells via clathrin-coated pit endocytosis. In vivo, JMJD6-FMDV exhibited preference for JMJD6(+) cells, but availability of this alternative receptor likely depends on route of inoculation. (C) 2016 Published by Elsevier Inc. C1 [Lawrence, Paul; Pacheco, Juan; Stenfeldt, Carolina; Arzt, Jonathan; Rai, Devendra K.; Rieder, Elizabeth] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. RP Rieder, E (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, NAA, POB 848, Greenport, NY 11944 USA. EM Elizabeth.Rieder@ars.usda.gov OI Stenfeldt, Carolina/0000-0002-2074-3886; Pacheco, Juan/0000-0001-5477-0201 FU CRIS projects, Agricultural Research Service (ARS), U.S. Department of Agriculture [1940-32000-057-00D]; United States Department of Agriculture FX Funding for the research detailed in this manuscript was provided through Congressionally allocated dollars for the Agricultural Research Service of the United States Department of Agriculture. Specifically, CRIS projects no. 1940-32000-057-00D, Agricultural Research Service (ARS), U.S. Department of Agriculture (Dr. Elizabeth Rieder). NR 69 TC 1 Z9 1 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAY PY 2016 VL 492 BP 108 EP 117 DI 10.1016/j.virol.2016.02.004 PG 10 WC Virology SC Virology GA DJ4WX UT WOS:000374209900013 PM 26914509 ER PT J AU Weber, MN Bauermann, FV Bayles, DO Canal, CW Neill, JD Ridpath, JF AF Weber, M. N. Bauermann, F. V. Bayles, D. O. Canal, C. W. Neill, J. D. Ridpath, J. F. TI Comparison of 'HoBi-like viral populations among persistent infected calves generated under experimental conditions and to inoculum virus SO VIROLOGY LA English DT Article DE Pestivirus; 'HoBi'-like; Persistent infection; Quasispecies; Viral swarms ID DIARRHEA VIRUS; ATYPICAL PESTIVIRUS; GLYCOPROTEIN E2; CATTLE; DISEASE; SERUM; OUTBREAK; REGION AB Like other members from the Pestivirus genus, 'HoBi-like pestiviruses cause economic losses for cattle producers due to both acute and persistent infections. The present study analyzed for the first time PI animals derived from a controlled infection with two different 'HoBi'-like strains where the animals were maintained under conditions where superinfection by other pestiviruses could be excluded. The sequence of the region coding for viral glycoproteins E1/E2 of variants within the swarms of viruses present in the PI calves and two viral inoculums used to generate them were compared. Differences in genetic composition of the viral swarms were observed suggesting that host factors can play a role in genetic variations among PIs. Moreover, PIs generated with the same inoculum showed amino acid substitutions in similar sites of the polyprotein, even in serum from PIs with different quasispecies composition, reinforcing that some specific sites in E2 are important for host adaptation. Published by Elsevier Inc. C1 [Weber, M. N.; Canal, C. W.] Univ Fed Rio Grande do Sul, Fac Vet, Virol Lab, Porto Alegre, RS, Brazil. [Bauermann, F. V.; Bayles, D. O.; Neill, J. D.; Ridpath, J. F.] ARS, Ruminant Dis & Immunol Res Unit, NADC, USDA, Ames, IA 50010 USA. RP Ridpath, JF (reprint author), ARS, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave,POB 70, Ames, IA 50010 USA. EM julia.ridpath@ars.usda.gov OI Canal, Claudio/0000-0002-0621-243X; Weber, Matheus/0000-0001-8282-6778 FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [99999.009963/2014-06] FX The authors thank Patricia Federico and Kathryn McMullen for their expert and invaluable technical support. Matheus Nunes Weber was sponsored with scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) (99999.009963/2014-06) during the execution of this study. NR 33 TC 3 Z9 3 U1 0 U2 4 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAY PY 2016 VL 492 BP 225 EP 231 DI 10.1016/j.virol.2016.03.001 PG 7 WC Virology SC Virology GA DJ4WX UT WOS:000374209900025 PM 26971244 ER PT J AU Jennings, DE Duan, JJ Bauer, LS Schmude, JM Wetherington, MT Shrewsbury, PM AF Jennings, David E. Duan, Jian J. Bauer, Leah S. Schmude, Jonathan M. Wetherington, Miles T. Shrewsbury, Paula M. TI Temporal dynamics of woodpecker predation on emerald ash borer (Agrilus planipennis) in the northeastern USA SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Buprestidae; Fraxinus; invasive species; Picidae; predation ID BEETLE ANOPLOPHORA-GLABRIPENNIS; COLEOPTERA BUPRESTIDAE; FRAXINUS SPP.; POPULATION-DYNAMICS; FORAGING BEHAVIOR; IMMATURE STAGES; MORTALITY; FAIRMAIRE; IMPACTS; ECOLOGY AB 1 Woodpeckers (Picidae) are important natural enemies attacking emerald ash borer (EAB) Agrilus planipennis Fairmaire in North America. There can be considerable variation in predation levels within and between sites, and among different times of year; therefore, understanding what causes these differences is necessary for effectively predicting EAB population dynamics. 2 We examined the temporal dynamics of woodpecker predation on EAB in Michigan and Maryland, as well as how they were affected by season, region, resource availability, tree size and crown condition. In Michigan, we quantified predation in association with EAB developmental stages on different trees over 2 years, whereas, in Maryland, we recorded woodpecker attacks on the same trees for 1 year. 3 Season was a significant predictor of woodpecker predation, with most occurring in winter when late-instar larvae were abundant. Predation also was affected by crown condition and tree size. Additionally, predation levels were similar throughout the year in a region where generations are considered to be less synchronized, representing a more consistent resource for woodpeckers. 4 The present study highlights the various factors affecting woodpecker predation over time. The results demonstrate the importance of multi-season studies of interactions between invasive species and native natural enemies when aiming to fully understand their dynamics. C1 [Jennings, David E.; Wetherington, Miles T.; Shrewsbury, Paula M.] Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. [Duan, Jian J.; Schmude, Jonathan M.] USDA ARS, Beneficial Insects Intro Res Unit, 501 South Chapel St, Newark, DE 19711 USA. [Bauer, Leah S.] USDA Forest Serv, No Res Stn, 3101 Technol Blvd, Lansing, MI 48910 USA. RP Jennings, DE (reprint author), Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. EM david.e.jennings@gmail.com OI Jennings, David/0000-0001-7179-3069 FU USDA National Institute of Food and Agriculture; McIntire-Stennis Project [1003486]; USDA ARS Specific Cooperative Agreement [58-1926-167] FX This work was supported by the USDA National Institute of Food and Agriculture, McIntire-Stennis Project 1003486 and USDA ARS Specific Cooperative Agreement (58-1926-167). We thank the Maryland Department of Agriculture, Mark Beals and Jesse Morgan (Green Ridge State Forest), Phil Taylor (USDA ARS), and Xiao-Yi Wang (Chinese Academy of Forestry) for logistical and field assistance. We are also grateful to Doug Luster (USDA ARS) and three anonymous reviewers for providing comments that greatly improved the manuscript. NR 44 TC 0 Z9 0 U1 11 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD MAY PY 2016 VL 18 IS 2 BP 174 EP 181 DI 10.1111/afe.12142 PG 8 WC Entomology SC Entomology GA DI8UO UT WOS:000373777800010 ER PT J AU Xie, J Zha, TS Zhou, CX Jia, X Yu, HQ Yang, B Chen, JQ Zhang, F Wang, B Bourqueg, CPA Sun, G Ma, H Liu, H Peltolad, H AF Xie, Jing Zha, Tianshan Zhou, Caixian Jia, Xin Yu, Haiqun Yang, Bai Chen, Jiquan Zhang, Feng Wang, Ben Bourqueg, Charles P. -A. Sun, Ge Ma, Hong Liu, He Peltolad, Heli TI Seasonal variation in ecosystem water use efficiency in an urban-forest reserve affected by periodic drought SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Climate warming; Eddy covariance; Evapotranspiration; Extreme drought; Gross ecosystem production ID CARBON FLUXES; EDDY COVARIANCE; ENVIRONMENTAL CONTROLS; TEMPERATE FOREST; CLIMATE-CHANGE; SOIL-MOISTURE; ASPEN FOREST; LONG-TERM; VEGETATION; CHINA AB The impact of extreme weather events on water-carbon coupling and ecosystem water use efficiency (WUE) in arid to semi-arid conditions is poorly understood. Evapotranspiration (ET) and gross ecosystem production (GEP) were based on continuously eddy-covariance measurements taken over an urban forest reserve in Beijing, in a 3-year period (2012-2014) to calculate WUE (GEP:ET). Our objective was to investigate the seasonal response of WUE to changing environmental and drought conditions at different timescales. Annually, the forest produced new plant biomass at 2.6 +/- 0.2 g C per kg of water loss. Within each season, interactions of surface conductance (g(c)) and normalized difference vegetation index (NDVI; i.e., g(c) x NDVI) in spring, net radiation (R-n) and air temperature (T-a; i.e., R-n x T-a) in summer, and Rn and vapor pressure deficit (D; i.e., R-n, x D) in autumn were found as the significant variables explaining seasonal variation in WUE. Daily WUE correlated positively with T-a and NDVI during the growing season, but a negative relationship during excessively dry periods (i.e., 2014). Daily WUE decreased during warm and dry days or remained nearly constant at low levels due to proportional decreases in GEP and ET. An extreme drought during the leaf expansion led to a greater decline in GEP than in ET, causing WUE to be lower in 2012 and 2014 than that in 2013. In contrast, an extreme drought during the leaf coloration led to a greater decline in ET than in GEP, causing higher WUE in 2013 and 2014 than that in 2012. We concluded that: (i) high soil water content (SWC) during leaf expansion was more important than high SWC in mid-summer or autumn for maintaining a high seasonal WUE; and that (ii) seasonal water availability combined with variable drought severity and duration during periods of changing T-a, caused seasonal ET and GEP to respond differently, introducing significant variation in seasonal WUE. (C) 2016 Elsevier B.V. All rights reserved. C1 [Xie, Jing; Zha, Tianshan; Jia, Xin; Wang, Ben] Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing 100083, Peoples R China. [Xie, Jing; Zha, Tianshan; Jia, Xin; Wang, Ben] Beijing Forestry Univ, Minist Educ, Key Lab Soil & Water Conservat & Desertificat Com, Beijing 100083, Peoples R China. [Xie, Jing; Zhou, Caixian; Yu, Haiqun; Zhang, Feng; Ma, Hong] Beijing Forestry Carbon Sequestrat Adm, Beijing 100013, Peoples R China. [Jia, Xin; Wang, Ben; Peltolad, Heli] Univ Eastern Finland, Sch Forest Sci, POB 111, FIN-80101 Joensuu, Finland. [Yang, Bai] Oak Ridge Natl Lab, Div Environm Sci, POB 2008, Oak Ridge, TN 37831 USA. [Chen, Jiquan] Michigan State Univ, CGCEO Geog, E Lansing, MI 48823 USA. [Bourqueg, Charles P. -A.] Univ New Brunswick, Fac Forestry & Environm Management, POB 4400, Fredericton, NB E3B 5A3, Canada. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Liu, He] Beijing Forestry Univ, Beijing 100083, Peoples R China. RP Zha, TS (reprint author), Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing 100083, Peoples R China. EM tianshanzha@bjfu.edu.cn RI Chen, Jiquan/D-1955-2009 FU National Natural Science Foundation of China (NSFC) [31361130340, 31270755]; Beijing Science and Technology Project China Postdoctoral Science Foundation [z141100006014031, 2013M540055]; Academy of Finland [14921] FX This research was supported by grants from National Natural Science Foundation of China (NSFC; 31361130340, 31270755), the Beijing Science and Technology Project (z141100006014031) China Postdoctoral Science Foundation funded project (2013M540055), and the Academy of Finland (Project No. 14921). This work is related to the ongoing Finnish Chinese research collaboration project EXTREME, between the Beijing Forestry University (BJFU) and the University of Eastern Finland (UEF). The U.S.-China Carbon Consortium (USCCC) promoted this work via helpful discussions and the exchange of ideas. We thank Peng Liu and Cai Ren for their assistances with field measurements and instrumentation maintenances and Gabriela Shirkey for editing the language of the manuscript. NR 35 TC 1 Z9 1 U1 8 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD MAY 1 PY 2016 VL 221 BP 142 EP 151 DI 10.1016/j.agrformet.2016.02.013 PG 10 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA DJ2ZM UT WOS:000374074200012 ER PT J AU Rich, JO Manitchotpisit, P Peterson, SW Liu, SQ Leathers, TD Anderson, AM AF Rich, Joseph O. Manitchotpisit, Pennapa Peterson, Stephen W. Liu, Siqing Leathers, Timothy D. Anderson, Amber M. TI Phylogenetic classification of Aureobasidium pullulans strains for production of feruloyl esterase SO BIOTECHNOLOGY LETTERS LA English DT Article DE Aureobasidium pullulans; Color variant; Ferulic acid; Phylogeny; Pullulan ID POLYSACCHARIDE PRODUCTION; COLOR VARIANTS; FERMENTATION; CARBON; CELLS; OIL AB The objective was to phylogenetically classify diverse strains of Aureobasidium pullulans and determine their production of feruloyl esterase. Seventeen strains from the A. pullulans literature were phylogenetically classified. Phenotypic traits of color variation and endo-beta-1,4-xylanase overproduction were associated with phylogenetic clade 10 and particularly clade 8. Literature strains used for pullulan production all belonged to clade 7. These strains and 36 previously classified strains were tested for feruloyl esterase production, which was found to be associated with phylogenetic clades 4, 11, and particularly clade 8. Clade 8 strains NRRL 58552 and NRRL 62041 produced the highest levels of feruloyl esterase among strains tested. Production of both xylanase and feruloyl esterase are associated with A. pullulans strains in phylogenetic clade 8, which is thus a promising source of enzymes with potential biotechnological applications. C1 [Rich, Joseph O.; Liu, Siqing; Leathers, Timothy D.; Anderson, Amber M.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Manitchotpisit, Pennapa] Illinois State Univ, Sch Biol Sci, Normal, IL 61761 USA. [Peterson, Stephen W.] ARS, Mycotoxins & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Leathers, TD (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Tim.Leathers@ars.usda.gov NR 38 TC 0 Z9 0 U1 7 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0141-5492 EI 1573-6776 J9 BIOTECHNOL LETT JI Biotechnol. Lett. PD MAY PY 2016 VL 38 IS 5 BP 863 EP 870 DI 10.1007/s10529-016-2054-y PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DI9YC UT WOS:000373857600018 PM 26875091 ER PT J AU Girsova, NV Bottner-Parker, KD Bogoutdinov, DZ Meshkov, YI Mozhaeva, KA Kastalyeva, TB Lee, IM AF Girsova, Natalia V. Bottner-Parker, Kristi D. Bogoutdinov, Damir Z. Meshkov, Yuri I. Mozhaeva, Karina A. Kastalyeva, Tatyana B. Lee, Ing Ming TI Diverse phytoplasmas associated with potato stolbur and other related potato diseases in Russia SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Potato purple top; Round leaf disease; Phytoplasma group 16SrXII; Phytoplasma group 16SrIII; Phytoplasma group 16SrVI; Phytoplasma group 16SrI ID 16S RIBOSOMAL-RNA; POLYMERASE-CHAIN-REACTION; CLASSIFICATION; IDENTIFICATION; YELLOWS; AMPLIFICATION; WASHINGTON; MOLLICUTES; ORGANISMS; GENES AB A large scale survey on diseased potato plants that exhibited symptoms similar to those of stolbur, potato purple top wilt, potato witches'-broom, and potato round-leaf in eight Economical Regions of Russia was conducted from 2006 to 2012. A total of 1228 potatoes were collected and the associated phytoplasmas were identified. Phytoplasmas belonging to five 16S rRNA (16Sr) phytoplasma groups (16SrI, 16SrII, 16SrIII, 16SrVI, and 16SrXII) and at least eight subgroups (16SrI-B, 16SrI-C, 16SrI-P, 16SrII-A, 16SrIII-B, 16SrVI-A, 16SrVI-C, and 16SrXII-A) were identified. To our knowledge, subgroup 16SrIII-B and 16SrVI-C phytoplasmas have not been reported to infect potatoes. Based on the results of the survey, the percentages of infection caused by various phytoplasmas were: 40.2 % (16SrXII), 25.5 % (16SrIII), 20.9 % (16SrI), 12.4 % (16SrVI), and 1.2 % (16SrII). The results also indicated potato diseases exhibiting stolbur or similar symptoms could be caused by stolbur phytoplasma (16SrXII-A) alone or by other phytoplasma groups (e.g., 16SrI and 16SrIII). A survey on potential insect vectors was conducted from 2009 to 2012 in the Moscow oblast of the Central Region. A total of 23 different species of Hemiptera insects were screened for the presence of phytoplasmas. Eight leafhopper and three froghopper species carried phytoplasmas. Euscelis incisus and Macrosteles laevis were the most prevalent. The other species were present in very low populations. C1 [Girsova, Natalia V.; Meshkov, Yuri I.; Mozhaeva, Karina A.; Kastalyeva, Tatyana B.] Russian Res Inst Phytopathol, Bolshie Vyaziomy 143050, Moscow Oblast, Russia. [Bottner-Parker, Kristi D.; Lee, Ing Ming] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. [Bogoutdinov, Damir Z.] Samara State Agr Acad, Kinel 446442, Samara Oblast, Russia. RP Kastalyeva, TB (reprint author), Russian Res Inst Phytopathol, Bolshie Vyaziomy 143050, Moscow Oblast, Russia.; Lee, IM (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD USA. EM kastalyeva@vniif.ru; ingming.lee@ars.usda.gov FU ISTC Russia-US collaborative project [3468] FX This research was funded under the ISTC Russia-US collaborative project #3468. NR 54 TC 0 Z9 1 U1 3 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD MAY PY 2016 VL 145 IS 1 BP 139 EP 153 DI 10.1007/s10658-015-0824-3 PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA DJ1VW UT WOS:000373994100013 ER PT J AU Thomma, BPHJ Seidl, MF Shi-Kunne, X Cook, DE Bolton, MD van Kan, JAL Faino, L AF Thomma, Bart P. H. J. Seidl, Michael F. Shi-Kunne, Xiaoqian Cook, David E. Bolton, Melvin D. van Kan, Jan A. L. Faino, Luigi TI Mind the gap; seven reasons to close fragmented genome assemblies SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Fungal genome; Next-generation sequencing; Assembly; Transposable element; Repeat ID FUNGUS NEUROSPORA-CRASSA; TRANSPOSABLE ELEMENTS; LTR RETROTRANSPOSONS; FILAMENTOUS FUNGI; PATHOGENIC FUNGI; GENE-EXPRESSION; REPETITIVE DNA; CHROMOSOME; EVOLUTION; SEQUENCE AB Like other domains of life, research into the biology of filamentous microbes has greatly benefited from the advent of whole-genome sequencing. Next-generation sequencing (NGS) technologies have revolutionized sequencing, making genomic sciences accessible to many academic laboratories including those that study non-model organisms. Thus, hundreds of fungal genomes have been sequenced and are publically available today, although these initiatives have typically yielded considerably fragmented genome assemblies that often lack large contiguous genomic regions. Many important genomic features are contained in intergenic DNA that is often missing in current genome assemblies, and recent studies underscore the significance of non-coding regions and repetitive elements for the life style, adaptability and evolution of many organisms. The study of particular types of genetic elements, such as telomeres, centromeres, repetitive elements, effectors, and clusters of co-regulated genes, but also of phenomena such as structural rearrangements, genome compartmentalization and epigenetics, greatly benefits from having a contiguous and high-quality, preferably even complete and gapless, genome assembly. Here we discuss a number of important reasons to produce gapless, finished, genome assemblies to help answer important biological questions. (C) 2015 Elsevier Inc. All rights reserved. C1 [Thomma, Bart P. H. J.; Seidl, Michael F.; Shi-Kunne, Xiaoqian; Cook, David E.; van Kan, Jan A. L.; Faino, Luigi] Wageningen Univ, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. [Bolton, Melvin D.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. RP Thomma, BPHJ (reprint author), Wageningen Univ, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. EM bart.thomma@wur.nl OI Seidl, Michael F/0000-0002-5218-2083; van Kan, Jan/0000-0003-3563-1550; faino, luigi/0000-0002-6807-4191 FU Research Council Earth and Life Sciences (ALW) of the Netherlands Organization of Scientific Research (NWO); Human Frontiers in Science Program (HFSP) Long-term fellowship [LT000627/2014-L] FX Work in the laboratory of BPHJT is support by the Research Council Earth and Life Sciences (ALW) of the Netherlands Organization of Scientific Research (NWO). Funding was provided to DEC by the Human Frontiers in Science Program (HFSP) Long-term fellowship (LT000627/2014-L). NR 89 TC 7 Z9 7 U1 5 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 EI 1096-0937 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD MAY PY 2016 VL 90 BP 24 EP 30 DI 10.1016/j.fgb.2015.08.010 PG 7 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA DI8KN UT WOS:000373750000004 PM 26342853 ER PT J AU Funnell-Harris, DL O'Neill, PM Sattler, SE Yerka, MK AF Funnell-Harris, Deanna L. O'Neill, Patrick M. Sattler, Scott E. Yerka, Melinda K. TI Response of Sweet Sorghum Lines to Stalk Pathogens Fusarium thapsinum and Macrophomina phaseolina SO PLANT DISEASE LA English DT Article ID CHARCOAL ROT FUNGUS; BICOLOR L. MOENCH; GRAIN-SORGHUM; ASCOCHYTA BLIGHT; PLANT COLOR; RESISTANCE; TEMPERATURE; SUGAR; GROWTH; ALTERNARIA AB Sweet sorghum (Sorghum bicolor (L.) Moench) has potential for bioenergy. It is adapted to a variety of U.S. locations and the extracted juice can be directly fermented into ethanol. However, little research on fungal stalk rots, diseases that pose serious constraints for yield and quality of juice and biomass, has been reported. A greenhouse bioassay was designed to assess charcoal rot (Macrophomina phaseolina) and Fusarium stalk rot (Fusarium thapsinum) in plants at maturity, the developmental stage at which these diseases are manifested. Multiple plantings of a susceptible grain line, RTx430, were used as a control for variation in flowering times among sweet sorghum lines. Lesion length measurements in inoculated peduncles were used to quantify disease severity. Sweet sorghum lines 'Rio' and `M81E' exhibited resistance to F. thapsinum and M. phaseolina, respectively; and, in contrast, 'Colman' sorghum exhibited susceptibility to both pathogens. Lesion development over time in Colman was monitored. These results will enhance molecular and biochemical analyses of responses to pathogens, and breeding stalk-rot-resistant sweet sorghum lines. C1 [Funnell-Harris, Deanna L.; O'Neill, Patrick M.; Sattler, Scott E.; Yerka, Melinda K.] Univ Nebraska, USDA ARS, GFBRU, Lincoln, NE 68583 USA. [Funnell-Harris, Deanna L.; O'Neill, Patrick M.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. [Sattler, Scott E.; Yerka, Melinda K.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. RP Funnell-Harris, DL (reprint author), Univ Nebraska, USDA ARS, GFBRU, Lincoln, NE 68583 USA.; Funnell-Harris, DL (reprint author), Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. EM Deanna.Funnell-Harris@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service CRIS [5440-21220-032-00D] FX We thank J. Toy for production of greenhouse-grown grain, T. Gries and S. Finegan for maintenance of plants and assistance with inoculations, and K. Steinhauer and M. Sederberg for laboratory and greenhouse assistance. This work was supported by United States Department of Agriculture-Agricultural Research Service CRIS project number 5440-21220-032-00D. NR 61 TC 0 Z9 0 U1 5 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 896 EP 903 DI 10.1094/PDIS-09-15-1050-RE PG 8 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100004 ER PT J AU Alcala, AVC Paulitz, TC Schroeder, KL Porter, LD Derie, ML du Toit, LJ AF Alcala, Ana Vida C. Paulitz, Timothy C. Schroeder, Kurtis L. Porter, Lyndon D. Derie, Michael L. du Toit, Lindsey J. TI Pythium Species Associated with Damping-off of Pea in Certified Organic Fields in the Columbia Basin of Central Washington SO PLANT DISEASE LA English DT Article ID POLYMERASE-CHAIN-REACTION; ROOT-ROT; SOIL; WHEAT; SPP.; DISEASE; SEED; IDENTIFICATION; PATHOGENICITY; TEMPERATURE AB Organic vegetable production accounted for 19% of the total organic acreage in Washington State in 2013, with 1,700 ha of certified organic vegetable pea. However, production is challenged constantly with the threat of poor emergence after planting due to damping-off caused by Pythium spp. A survey of Pythium spp. in organic vegetable production areas of the semiarid Columbia Basin of central Washington was carried out in fall 2009 to identify species associated with damping-off during early spring planting. Of 305 isolates baited from soil sampled from 37 certified organic fields, 264 were identified to 16 Pythium spp. by sequencing the internal transcribed spacer region of ribosomal DNA. A soil DNA-CFU regression curve was developed using real-time quantitative polymerase chain reaction assays for each of the three predominant pathogenic species (Pythium abappressorium, the P. irregulare complex, and P. ultimum var. ultimum) found in soil sampled from the 37 fields The P. irregulare complex, P. abappressorium, and P. ultimum var. ultimum were detected in 57, 78, and 100% of the fields sampled, respectively. A regression analysis was used to determine that P. ultimum var. ultimum ranged from 14 to 332 CFU/g of soil in the 37 fields, the P. irregulare complex ranged from 25 to 228 CFU/g of soil, and P. abappressorium DNA was below the quantifiable limit. In summary, P. ultimum var. ultimum was the most prevalent pathogenic Pythium sp. detected in certified organic fields in the semiarid Columbia Basin of central Washington but multiple Pythium spp. may be associated with damping-off in cool and wet, early spring planting conditions. C1 [Alcala, Ana Vida C.; Derie, Michael L.; du Toit, Lindsey J.] Washington State Univ Mt Vernon NWREC, Mt Vernon, WA 98273 USA. [Paulitz, Timothy C.; Schroeder, Kurtis L.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Porter, Lyndon D.] USDA ARS, Prosser, WA 99350 USA. [Schroeder, Kurtis L.] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. RP du Toit, LJ (reprint author), Washington State Univ Mt Vernon NWREC, Mt Vernon, WA 98273 USA. EM dutoit@wsu.edu OI Paulitz, Timothy/0000-0002-8885-3803 FU Department of Plant Pathology (PPNS) [0691]; College of Agricultural, Human, and Natural Resource Sciences; Agricultural Research Center (Hatch Project) [WRN05595]; Center for Sustaining Agriculture and Natural Resources BIOAg program at Washington State University FX This project was supported by the Department of Plant Pathology (PPNS number 0691); the College of Agricultural, Human, and Natural Resource Sciences; the Agricultural Research Center (Hatch Project number WRN05595); and the Center for Sustaining Agriculture and Natural Resources BIOAg program at Washington State University. NR 46 TC 0 Z9 0 U1 6 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 916 EP 925 DI 10.1094/PDIS-07-15-0774-RE PG 10 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100006 ER PT J AU Wan, AM Chen, XM Yuen, J AF Wan, Armin Chen, Xianming Yuen, Jonathan TI Races of Puccinia striiformis f. sp tritici in the United States in 2011 and 2012 and Comparison with Races in 2010 SO PLANT DISEASE LA English DT Article ID WHEAT STRIPE RUST; ADULT-PLANT RESISTANCE; VIRULENCE CHARACTERIZATION; PHYSIOLOGICAL RACES; OCTAL NOTATION; PATHOGENS; GENE AB Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases on wheat in the United States. In 2011, severe wheat stripe rust caused extensive application of fungicides in the western United States, and the disease was more widespread and caused more yield loss in the eastern United States in 2012. In this study, we characterized virulences and identified races of P. striiformis f. sp. tritici by testing the stripe rust samples collected throughout the United States in 2011 and 2012 on a set of 18 Yr single-gene differentials. In 2011, 35 races were identified from 349 viable samples collected from 19 states of the United States and Ontario province of Canada, with PSTv-11 (35.5%), PSTv-37 (12.6%), PSTv-14 (11.8%), PSTv-4 (5.4%), and PSTv-34 (3.4%) as the top five predominant races. In 2012, 23 races were identified from 341 viable samples collected from 24 states of the United States and Ontario of Canada, with PSTv-37 (47.5%), PSTv-11 (11.7%), PSTv-14 (10.0%), PSTv-52 (9.4%), and PSTv-48 (4.4%) as the top five predominant races. Nationally, PSTv-37, PSTv-52, and PSTv-34 were most widely distributed, while PSTv-11, PSTv-14, PSTv-4, and PSTv-48 were mostly detected in the western United States. High frequencies(>80%) were detected for virulences to Yr6, Yr7, Yr8, Yr9, Yr17, Yr27, Yr44, and YrExp2; moderate frequencies (20 to 80%) for virulences to Yr1, Yr43, YrTr1, and YrTye; low frequencies (<10%) for virulences to Yr10, Yr24, Yr32, and YrSP; and virulences to Yr5 and Yr15 were not detected, indicating that these two genes are still effective against the P. striiformis f. sp. tritici population in the United States. Both positive and negative associations were identified between some of the virulences. In total, 55 races identified from 2010 to 2012 in the United States were clustered into two major virulence groups, and dynamics of predominant races and virulence frequencies for the 3 years were presented and discussed. This information is useful for making decisions when screening wheat germplasm for developing stripe-rust-resistant wheat cultivars and managing the disease by growing cultivars with adequate and durable resistance. The severe epidemics and the occurrence of the large number of races in the 3 years indicate that efforts should be made to use diverse resistance genes, especially to combine effective all-stage resistance genes with genes for high-temperature adult-plant resistance. C1 [Wan, Armin] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Yuen, Jonathan] Swedish Univ Agr Sci, Dept Forest Mycol & Plant Pathol, S-75007 Uppsala, Sweden. RP Chen, XM (reprint author), Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA.; Chen, XM (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM xianming@wsu.edu FU United States Department of Agriculture Agricultural Research Service [5348-22000-015-00D]; Washington Wheat Commission; Idaho Wheat Commission FX This study was supported by the United States Department of Agriculture Agricultural Research Service (project number 5348-22000-015-00D), Washington Wheat Commission, and Idaho Wheat Commission. We thank the following collaborators for collecting and sending stripe rust samples in 2011 and 2012: Z. Abate, M. Acevedo, C. Aitken, T. Allen, J. Baker, J. Barnhill, A. B arta, G. Bergstrom, D. Bland, B. Blessing, M. Bonman, R. Bowden, C. Bradley, M. Burrows, K. Campbell, M. Carson, J. Chatelain, J. Chen, G. Cisar, B. Conrady, T. Gordon, G. Davies, E. De Wolf, J. Dubcovsky, R. Ellis, M. Eruin, A. Esser, B. Fanning, A. Fox, A. Greea, C. Griffey, A. Harber, S. Harrison, P. Hayes, R. Herrington, D. Hershman, B. Hunger, C. Jackson, L. Jackson, Y. Jin, D. Johnson, J. Kautz, M. Kolding, J. Kolmer, D. Long, I. Lowe, J. Marshall, S. Martinez, E. Miller, M. Miller, E. Milus, L. Morscheck, T. Murray, M. Newcomb, C. Nischwitz, M. Pace, B. Padgett, M. Pumphery, S. Reinertsen, L. Roach, J. Rouse, M. Rouse, M. Rugg, L. Skoglund, B. Simoneaux, J. Sthapit, B. Stougaard, A. Tenuta, S. Thompson, R. Webb, S. Wegulo, H. Wetzel, K. Wise, D. Wysock, J. Youmans, and B. Zemetra. NR 30 TC 6 Z9 6 U1 8 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 966 EP 975 DI 10.1094/PDIS-10-15-1122-RE PG 10 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100013 ER PT J AU Pereira, TBC Daily, EL Davis, RE Banzato, TC Galvao, SR Bedendo, IP AF Pereira, T. B. C. Daily, E. L. Davis, R. E. Banzato, T. C. Galvao, S. R. Bedendo, I. P. TI Cauliflower is a New Host of a Subgroup 16SrVII-B Phytoplasma Associated with Stunting Disease in Brazil. SO PLANT DISEASE LA English DT News Item ID CLASSIFICATION; YELLOWS C1 [Pereira, T. B. C.; Banzato, T. C.; Galvao, S. R.; Bedendo, I. P.] Univ Sao Paulo, ESALQ, Dept Plant Pathol & Nematol, BR-13418900 Piracicaba, SP, Brazil. [Daily, E. L.; Davis, R. E.] USDA ARS, MPPL, Beltsville, MD 20705 USA. RP Pereira, TBC (reprint author), Univ Sao Paulo, ESALQ, Dept Plant Pathol & Nematol, BR-13418900 Piracicaba, SP, Brazil. NR 5 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1007 EP 1007 DI 10.1094/PDIS-09-15-1110-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100019 ER PT J AU Bruckart, WL Michael, JL Coombs, EM Pirosko, CB AF Bruckart, W. L., III Michael, J. L. Coombs, E. M. Pirosko, C. B. TI Rust Pathogen Puccinia jaceae is Established on Yellow Starthistle (Centaurea solstitialis) in Oregon. SO PLANT DISEASE LA English DT News Item C1 [Bruckart, W. L., III; Michael, J. L.] USDA ARS FDWSRU, Ft Detrick, MD 21702 USA. [Coombs, E. M.; Pirosko, C. B.] Oregon Dept Agr, Salem, OR 97301 USA. RP Bruckart, WL (reprint author), USDA ARS FDWSRU, Ft Detrick, MD 21702 USA. NR 5 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1009 EP 1009 DI 10.1094/PDIS-09-15-1042-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100024 ER PT J AU Demers, JE Byrne, JM Castlebury, LA AF Demers, J. E. Byrne, J. M. Castlebury, L. A. TI First Report of Crown Rust (Puccinia coronata var. gibberosa) on Blue Oat Grass (Helictotrichon sempervirens) in the United States. SO PLANT DISEASE LA English DT News Item C1 [Demers, J. E.; Castlebury, L. A.] USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Byrne, J. M.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Demers, JE (reprint author), USDA ARS, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. NR 3 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1009 EP 1009 DI 10.1094/PDIS-09-15-1093-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100023 ER PT J AU Yan, GP Khan, M Huang, D Lai, X Handoo, ZA AF Yan, G. P. Khan, M. Huang, D. Lai, X. Handoo, Z. A. TI First Report of the Stubby Root Nematode Paratrichodorus allius on Sugar Beet in Minnesota. SO PLANT DISEASE LA English DT News Item C1 [Yan, G. P.; Khan, M.; Huang, D.; Lai, X.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Handoo, Z. A.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. RP Yan, GP (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. NR 4 TC 1 Z9 1 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1022 EP 1022 DI 10.1094/PDIS-10-15-1231-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100057 ER PT J AU Yan, GP Plaisance, A Huang, D Gudmestad, NC Handoo, ZA AF Yan, G. P. Plaisance, A. Huang, D. Gudmestad, N. C. Handoo, Z. A. TI First Report of the Root-lesion Nematode Pratylenchus scribneri Infecting Potato in North Dakota. SO PLANT DISEASE LA English DT News Item C1 [Yan, G. P.; Plaisance, A.; Huang, D.; Gudmestad, N. C.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Handoo, Z. A.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. RP Yan, GP (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. NR 5 TC 1 Z9 1 U1 3 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1023 EP 1023 DI 10.1094/PDIS-10-15-1227-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100059 ER PT J AU Grunwald, NJ Larsen, MM Kamvar, ZN Reeser, PW Kanaskie, A Laine, J Wiese, R AF Gruenwald, N. J. Larsen, M. M. Kamvar, Z. N. Reeser, P. W. Kanaskie, A. Laine, J. Wiese, R. TI First Report of the EU1 Clonal Lineage of Phytophthora ramorum on Tanoak in an Oregon Forest. SO PLANT DISEASE LA English DT News Item ID OAK DEATH PATHOGEN C1 [Gruenwald, N. J.; Larsen, M. M.; Kamvar, Z. N.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Reeser, P. W.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Kanaskie, A.; Laine, J.; Wiese, R.] Oregon Dept Forestry, Salem, OR 97310 USA. RP Grunwald, NJ (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RI Kamvar, Zhian/C-5236-2017; OI Kamvar, Zhian/0000-0003-1458-7108; Grunwald, Niklaus/0000-0003-1656-7602 NR 5 TC 0 Z9 0 U1 3 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1024 EP 1024 DI 10.1094/PDIS-10-15-1169-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100062 ER PT J AU Delgado, HVE Kaye, C Hincapie, M Boukari, W Wei, C Fernandez, JV Mollov, D Comstock, JC Rott, P AF Delgado, H. V. Espinoza Kaye, C. Hincapie, M. Boukari, W. Wei, C. Fernandez, J. V. Mollov, D. Comstock, J. C. Rott, P. TI First Report of Sugarcane yellow leaf virus Infecting Columbus Grass (Sorghum almum) in Florida. SO PLANT DISEASE LA English DT News Item C1 [Delgado, H. V. Espinoza; Hincapie, M.; Boukari, W.; Wei, C.; Rott, P.] Univ Florida, Everglades Res & Educ Ctr, IFAS, Dept Plant Pathol, Belle Glade, FL 33430 USA. [Kaye, C.] US Sugar Corp, Clewiston, FL 33440 USA. [Fernandez, J. V.] Univ Florida, Everglades Res & Educ Ctr, IFAS, Dept Agron, Belle Glade, FL 33430 USA. [Mollov, D.] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Comstock, J. C.] USDA ARS, Sugarcane Field Stn, Canal Point, FL 33438 USA. RP Delgado, HVE (reprint author), Univ Florida, Everglades Res & Educ Ctr, IFAS, Dept Plant Pathol, Belle Glade, FL 33430 USA. NR 5 TC 1 Z9 1 U1 4 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1027 EP 1028 DI 10.1094/PDIS-10-15-1158-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100072 ER PT J AU Alderman, SC Martin, RC Gilmore, BS Martin, RR Hoffman, GD Sullivan, CS Anderson, NP AF Alderman, S. C. Martin, R. C. Gilmore, B. S. Martin, R. R. Hoffman, G. D. Sullivan, C. S. Anderson, N. P. TI First Report of Cocksfoot mottle virus Infecting Dactylis glomerata in Oregon and the United States. SO PLANT DISEASE LA English DT News Item C1 [Alderman, S. C.; Martin, R. C.; Gilmore, B. S.] USDA ARS, FSCRU, Corvallis, OR 97331 USA. [Martin, R. R.] USDA ARS, HCRU, Corvallis, OR 97330 USA. [Hoffman, G. D.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Sullivan, C. S.] Linn Cty Extens, Tangent, OR 97389 USA. [Anderson, N. P.] Yamhill Cty Extens, Mcminnville, OR 97128 USA. RP Alderman, SC (reprint author), USDA ARS, FSCRU, Corvallis, OR 97331 USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2016 VL 100 IS 5 BP 1030 EP 1031 DI 10.1094/PDIS-09-15-1017-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DJ1ME UT WOS:000373966100080 ER PT J AU Vahling-Armstrong, C Dung, JKS Humann, JL Schroeder, BK AF Vahling-Armstrong, C. Dung, J. K. S. Humann, J. L. Schroeder, B. K. TI Effects of postharvest onion curing parameters on bulb rot caused by Pantoea agglomerans, Pantoea ananatis and Pantoea allii in storage SO PLANT PATHOLOGY LA English DT Article DE bulb rot; onion curing; Pantoea agglomerans; Pantoea allii; Pantoea ananatis ID TRANSMISSION; DISEASE; THRIPS; AGENT; SEED AB Crop loss of onion bulbs during storage carries an exceptionally high economic impact because a large portion of the production expenses has been expended before storage. Because of this, it is important to define practices that can reduce onion bulb losses caused by storage rots. This study investigates the impact of various curing parameters on disease development resulting from infection by Pantoea agglomerans, P. ananatis and P. allii on onion bulb cultivars Vaquero and Redwing, during storage. Overall, both the incidence and mean rot severity were similar amongst the bulbs under comparable conditions regardless of the species of Pantoea inoculated, although a significant difference was detected between the two onion bulb cultivars. In addition, a significant reduction of storage rot was observed when curing temperatures were 35 degrees C. At temperatures >35 degrees C, a shorter curing duration (2days vs 14days) decreased the severity of bulb rot due to Pantoea. This increased understanding of the inter-relationships between the parameters used for curing, and the incidence and severity of bulb rot caused by Pantoea helps provide guidance towards using the curing process as a means to reduce the level of damage resulting from post-harvest storage rot. C1 [Vahling-Armstrong, C.] USDA ARS, Ft Pierce, FL 34945 USA. [Dung, J. K. S.] Oregon State Univ, Madras, OR 97741 USA. [Humann, J. L.; Schroeder, B. K.] Washington State Univ, Pullman, WA 99164 USA. RP Schroeder, BK (reprint author), Washington State Univ, Pullman, WA 99164 USA. EM bschroeder@uidaho.edu FU Pacific Northwest Vegetable Association; Washington State Commission for Pesticide Registration; USDA Western Region Integrated Pest Management Competitive Grants Program; USDA-NIFA Specialty Crop Research Initiative [2010-01193]; Department of Plant Pathology in the Washington State University College of Agricultural, Human and Natural Resource Sciences; Washington State University Agricultural Research Center for CRIS Project [WNPOO652] FX The authors gratefully acknowledge financial support for this project from the Pacific Northwest Vegetable Association, the Washington State Commission for Pesticide Registration, USDA Western Region Integrated Pest Management Competitive Grants Program, and USDA-NIFA Specialty Crop Research Initiative no. 2010-01193. Invaluable in-kind support was provided by L&L Ag Production in the form of onion bulbs for storage evaluations. Special thanks to Dr Ron Gitiatis for providing strains. In addition, technical support was provided by A. Jenny Knerr, Austin Bates and Stacy Mauzey. This project was supported by the Department of Plant Pathology in the Washington State University College of Agricultural, Human and Natural Resource Sciences, and the Washington State University Agricultural Research Center for CRIS Project no. WNPOO652. NR 19 TC 0 Z9 0 U1 6 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD MAY PY 2016 VL 65 IS 4 BP 536 EP 544 DI 10.1111/ppa.12438 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DJ1DQ UT WOS:000373943900002 ER PT J AU Zhao, N Lin, H Lan, SQ Jia, QD Chen, XL Guo, H Chen, F AF Zhao, Nan Lin, Hong Lan, Suque Jia, Qidong Chen, Xinlu Guo, Hong Chen, Feng TI VvMJE1 of the grapevine (Vitis vinifera) VvMES methylesterase family encodes for methyl jasmonate esterase and has a role in stress response SO PLANT PHYSIOLOGY AND BIOCHEMISTRY LA English DT Article DE Methylesterase; Methyl jasmonate; Grapevine; Homology modelling; Abiotic stress ID ACID CARBOXYL METHYLTRANSFERASE; SYSTEMIC ACQUIRED-RESISTANCE; PLANT INNATE IMMUNITY; INDOLE-3-ACETIC-ACID METHYLTRANSFERASE; BIOCHEMICAL-CHARACTERIZATION; ARABIDOPSIS; SALICYLATE; EXPRESSION; DEFENSE; ENZYME AB The known members of plant methyl esterase (MES) family catalyze the hydrolysis of a C-O ester linkage of methyl esters of several phytohormones including indole-3-acetic acid, salicylic acid and jasmonic acid. The genome of grapevine (Vitis vinifera) was found to contain 15 MES genes, designated VvMES1-15. In this report, VvMES5 was selected for molecular, biochemical and structural studies. VvMES5 is most similar to tomato methyl jasmonate esterase. E. coli-expressed recombinant VvMES5 displayed methyl jasmonate (MeJA) esterase activity, it was renamed VvMJE1. Under steady-state conditions, VvMJE1 exhibited an apparent Km value of 92.9 mu M with MeJA. VvMJE1 was also shown to have lower activity with methyl salicylate (MeSA), another known substrate of the MES family, and only at high concentrations of the substrate. To understand the structural basis of VvMJE1 in discriminating MeJA and MeSA, a homolog model of VvMJE1 was made using the X-ray structure of tobacco SABP2, which encodes for methyl salicylate esterase, as a template. Interestingly, two bulky residues at the binding site and near the surface of tobacco SABP2 are replaced by relatively small residues in VvMJE1. Such a change enables the accommodation of a larger substrate MeJA in VvMJE1. The expression of VvMJE1 was compared in control grape plants and grape plants treated with one of the three stresses: heat, cold and UV-B. While the expression of VvMJE1 was not affected by heat treatment, its expression was significantly up regulated by cold treatment and UV-B treatment. This result suggests that VvMJE1 has a role in response of grape plants to these two abiotic stresses. (C) 2016 Elsevier Masson SAS. All rights reserved. C1 [Zhao, Nan; Chen, Xinlu; Chen, Feng] Univ Tennessee, Dept Plant Sci, 252 Ellington Plant Sci Bldg,2431 Joe Johnson Dr, Knoxville, TN 37996 USA. [Lin, Hong; Lan, Suque] ARS, USDA, Crop Dis Pests & Genet Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. [Lan, Suque] Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang, Peoples R China. [Jia, Qidong; Chen, Feng] Univ Tennessee, Grad Sch Genome Sci & Technol, Knoxville, TN 37996 USA. [Guo, Hong] Univ Tennessee, Dept Biochem & Cellular & Mol Biol, Knoxville, TN 37996 USA. RP Zhao, N; Chen, F (reprint author), Univ Tennessee, Dept Plant Sci, 252 Ellington Plant Sci Bldg,2431 Joe Johnson Dr, Knoxville, TN 37996 USA. EM znan@utk.edu; fengc@utk.edu OI Chen, Xinlu/0000-0002-7560-6125 FU USDA [58-5302-6-153] FX We thank Ms. Parminder Sahota for technical support. This research was supported by USDA Specific Cooperative Agreement 58-5302-6-153 (to F.C.). NR 41 TC 1 Z9 1 U1 11 U2 23 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0981-9428 J9 PLANT PHYSIOL BIOCH JI Plant Physiol. Biochem. PD MAY PY 2016 VL 102 BP 125 EP 132 DI 10.1016/j.plaphy.2016.02.027 PG 8 WC Plant Sciences SC Plant Sciences GA DJ3CD UT WOS:000374081500015 PM 26934101 ER PT J AU Watt, TJ Duan, JJ Tallamy, DW Hough-Goldstein, J Ilvento, TW Yue, X Ren, H AF Watt, Timothy J. Duan, Jian J. Tallamy, Douglas W. Hough-Goldstein, Judy Ilvento, Thomas W. Yue, Xin Ren, Hui TI Reproductive and developmental biology of the emerald ash borer parasitoid Spathius galinae (Hymenoptera: Braconidae) as affected by temperature SO BIOLOGICAL CONTROL LA English DT Article DE Agrilus planipennis; Mass-rearing; Life tables; Biological control; Fecundity; Survival ID GONATOCERUS-ASHMEADI HYMENOPTERA; HOMALODISCA-COAGULATA HEMIPTERA; POPULATION-GROWTH RATES; RUSSIAN FAR-EAST; COLEOPTERA BUPRESTIDAE; LIFE-HISTORY; NUTRITIONAL RESOURCES; TUTA-ABSOLUTA; EGG; ECOLOGY AB Emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) is an invasive pest of serious concern in North America. To complement ongoing biological control efforts, Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae), a recently-described specialist parasitoid of emerald ash borer larvae, is being released for the first time in the United States. To better understand its reproductive and developmental biology, and to improve outcomes of mass-rearing, we investigated the effects of constant ambient temperature (15, 20, 25, 30, and 35 degrees C) on immature development time, sex ratio, pre-oviposition period, fecundity and survivorship. Results show that between 20 and 30 degrees C immature development time was inversely associated with temperature, with adults emerging approximate to 38, 32, and 25 d after oviposition at 20, 25, and 30 degrees C, respectively. At 15 degrees C larvae developed to 5th instars and spun cocoons before development was arrested, while at 35 degrees C parasitoid eggs desiccated and did not hatch. Sex ratio of adult progeny that were exposed to different temperatures, from eggs, was not affected. When newly (624 h-old) emerged female parasitoids were provided larval hosts throughout their lifetimes, adults parasitized at all temperatures except 35 degrees C, although parasitism rate was highest (35%), as was lifetime fecundity (43 progeny/female), when females lived at 25 degrees C. Survivorship was inversely associated with temperature, with ovipositing females living for >60 d when maintained at 15 degrees C, and females held at 35 degrees C living for <10 d. Life table analyses showed S. galinae had the highest net reproductive rate (R-0 = 27.8) and intrinsic rate of increase (r(m) = 0.29) when female parasitoids were maintained at 25 degrees C for their lifetimes, suggesting this is the optimal temperature to rear S. galinae. (C) 2016 Elsevier Inc. All rights reserved. C1 [Watt, Timothy J.; Tallamy, Douglas W.; Hough-Goldstein, Judy] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA. [Watt, Timothy J.; Duan, Jian J.] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Ilvento, Thomas W.; Yue, Xin; Ren, Hui] Univ Delaware, Dept Appl Econ & Stat, Newark, DE 19713 USA. RP Watt, TJ (reprint author), 77 Univ Ave, New Castle, DE 19720 USA. EM tjwatt@udel.edu FU United States Department of Agriculture-Animal and Plant Health Inspection Service Grant [58-1926-2-282] FX We thank Kristi Larson, Jackie Hoban, Sue Barth (University of Delaware), Jonathan Schmude and Cherie Keenan (United States Department of Agriculture-Agricultural Research Service) for assistance maintaining insect cultures. We are especially grateful to Dick Bean, Kimberly Rice, Charles Pickett, and Steve Bell (Maryland Department of Agriculture) for providing most of the ash logs used during this study. We also thank Mark Hoddle, Leigh Pilkington, and Michael Lewis (University of California-Riverside) for providing the framework for the bootstrap analysis. Finally, we thank Doug Luster (United States Department of Agriculture-Agricultural Research Service) for reviewing the manuscript. This research was supported through funding from United States Department of Agriculture-Animal and Plant Health Inspection Service Grant #58-1926-2-282. NR 44 TC 1 Z9 1 U1 8 U2 27 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2016 VL 96 BP 1 EP 7 DI 10.1016/j.biocontrol.2016.01.011 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DI3CY UT WOS:000373377000001 ER PT J AU Hay-Roe, MM Meagher, RL Nagoshi, RN Newman, Y AF Hay-Roe, Mirian M. Meagher, Robert L. Nagoshi, Rodney N. Newman, Yoana TI Distributional patterns of fall armyworm parasitoids in a corn field and a pasture field in Florida SO BIOLOGICAL CONTROL LA English DT Article DE Host strains; Agroecosystem; Dispersal behavior; Biological control ID SPODOPTERA-FRUGIPERDA LEPIDOPTERA; NOCTUIDAE HOST STRAINS; LANDSCAPE STRUCTURE; NATURAL ENEMIES; COMPLEX; HYMENOPTERA; POPULATIONS; EULOPHIDAE; DIVERSITY; SELECTION AB An assessment of parasitoids and their selective patterns among Spodoptera frugiperda corn and rice host strains was performed from August 2008 to August 2010 in a corn crop and a grass pasture in northern Florida under different seasonal conditions (spring and fall). Sentinel larvae from our laboratory were placed in both habitats. Results obtained in the corn crop differed from those in the pasture due to poor recovery rate of larvae in the pasture habitat. In corn fields, we found that the parasitoid community was composed of eight species of Hymenoptera and one Diptera species, which represented four families and two guilds. The more abundant parasitoid species in the corn field included Aleiodes laphygmae, Meteorus autographae, Cotesia sp., Euplectrus platyhypenae and Ophion flavidus while the Diptera species constituted the most abundant parasitoid in pastures. Among the parasitoid species collected in pastures were three Diptera species and four hymenoptera species. Parasitoid species did not discriminate among fall armyworm host strains, however they varied among the different habitats in the corn field. The ectoparasitoid Euplectrus platyhypenae and an unknown ichneumonid species were found primarily at the edges of the crop (in areas adjacent to the woods); while the endoparasitoids Cotesia sp. and Meteorus sp. were recovered more often in the main corn crop. Meteorus and Ophion were more abundant in the spring while the unknown ichneumonid was more abundant in the fall. Predator species differ among corn and pasture, which might contribute to the dynamics of parasitoid species in these fields. (C) 2016 Elsevier Inc. All rights reserved. C1 [Hay-Roe, Mirian M.; Meagher, Robert L.; Nagoshi, Rodney N.] ARS, Behav & Biocontrol Unit, USDA, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. [Hay-Roe, Mirian M.] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. [Newman, Yoana] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Newman, Yoana] Univ Wisconsin, Plant & Earth Sci, River Falls, WI 54022 USA. RP Hay-Roe, MM (reprint author), ARS, Behav & Biocontrol Unit, USDA, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM mmhr@ufl.edu NR 50 TC 0 Z9 0 U1 4 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2016 VL 96 BP 48 EP 56 DI 10.1016/j.biocontrol.2016.02.003 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DI3CY UT WOS:000373377000006 ER PT J AU Saadat, D Seraj, AA Goldansaz, SH Williams, L AF Saadat, Dorna Seraj, Ali Asghar Goldansaz, Seyed Hossein Williams, Livy, III TI Factors affecting reproductive success and life history parameters of Bracon hebetor Say (Hymenoptera: Braconidae) from three host-associated populations SO BIOLOGICAL CONTROL LA English DT Article DE Bracon hebetor; Ectomyelois ceratoniae; Braconidae; Carob moth; Augmentative biological control; Reproductive life history; Parasitism ID ECTOMYELOIS-CERATONIAE LEPIDOPTERA; MONOCTONUS-PAULENSIS HYMENOPTERA; OVIPOSITION BEHAVIOR; PARASITOID WASP; BURKINA-FASO; SEX-RATIOS; PYRALIDAE; QUALITY; SIZE; KUEHNIELLA AB Augmentative releases of native natural enemies are viable strategies for suppression of crop pests. Successful implementation of this approach requires in-depth knowledge of the natural enemy, its host (s), and the agroecosystem. In particular, appropriate mass rearing and release strategies rely on a thorough understanding of the reproductive biology of the natural enemy. In the present study, we evaluated the effects of parasitoid source (host habitat origin), fruit condition (i.e., ease of host access for parasitoid), impact of using an alternative host for rearing, and winter-simulated cold storage of hosts on the reproductive performance of Bracon hebetor. Several life history parameters, including attack behavior, proportion of paralyzed hosts, parasitism, oviposition, offspring sex ratio, and life table parameters were measured. Wasps originating from a pomegranate orchard-Ectomyelois ceratoniae host habitat generally had greater reproductive performance than those sourced from stored dry fruit-Plodia interpunctella or tomato field-Helicoverpa armigera habitats. Hosts infesting intact pomegranate fruit suffered greater levels of attack (e.g., paralysis, parasitism, and oviposition) than did more accessible hosts infesting damaged fruit. However, offspring sex ratio was generally more female-biased in the damaged fruit treatments. The developmental period of B. hebetor larvae and pupae was shorter (mean generation time) on Ephestia kuehniella hosts that had been subjected to a 2-month cold storage period (winter simulation) than untreated control hosts not subjected to cold storage. Sex ratio of offspring did not differ significantly between the treatments. Our studies demonstrated differential effects of wasp population source, and other biotic factors, on the reproductive biology of B. hebetor. Our current understanding suggests that efficacious protection of pomegranate crops with B. hebetor might be accomplished by using a rearing protocol that incorporates parasitoids originating from a pomegranate-E. ceratoniae source, followed by rearing on cold-treated E. kuehniella prior to release. Development of optimal release strategies is a topic for future studies. Published by Elsevier Inc. C1 [Saadat, Dorna; Seraj, Ali Asghar] Shahid Chamran Univ, Dept Plant Protect, Ahwaz, Iran. [Goldansaz, Seyed Hossein] Univ Tehran, Dept Plant Protect, Karaj, Iran. [Williams, Livy, III] ARS, USDA, European Biol Control Lab, Campus Int Baillarguet,CS 90013 Montferrier Lez, F-34988 St Gely Du Fesc, France. RP Williams, L (reprint author), ARS, USDA, USVL, 2700 Savannah Highway, Charleston, SC 29414 USA. EM livy.williams@ars.usda.gov FU excellent Center of Biological Control of the University of Tehran FX We appreciate the help of Dr. Klaus Horstmann for authoritative determination the parasitoid. We are grateful to P. A. Eliopoulos, M. N. Ghimire, and anonymous reviewers for their constructive comments on the manuscript. We also thank the pomegranate growers graciously permitted us access to their orchards. This study was partially supported by the excellent Center of Biological Control of the University of Tehran. NR 63 TC 1 Z9 1 U1 5 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2016 VL 96 BP 86 EP 92 DI 10.1016/j.biocontrol.2016.02.008 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DI3CY UT WOS:000373377000011 ER PT J AU Turner, TL Zhang, GC Oh, EJ Subramaniam, V Adiputra, A Subramaniam, V Skory, CD Jang, JY Yu, BJ Park, I Jin, YS AF Turner, Timothy L. Zhang, Guo-Chang Oh, Eun Joong Subramaniam, Vijay Adiputra, Andrew Subramaniam, Vimal Skory, Christopher D. Jang, Ji Yeon Yu, Byung Jo Park, In Jin, Yong-Su TI Lactic acid production from cellobiose and xylose by engineered Saccharomyces cerevisiae SO BIOTECHNOLOGY AND BIOENGINEERING LA English DT Article DE Saccharomyces cerevisiae; lactic acid; lactate dehydrogenase; cellobiose; metabolic engineering ID LACTATE-DEHYDROGENASE GENE; CELLULOSIC ETHANOL; BIOFUEL PRODUCTION; CELL-SURFACE; YEAST; FERMENTATION; GLUCOSE; METABOLISM; EVOLUTION; DELETION AB Efficient and rapid production of value-added chemicals from lignocellulosic biomass is an important step toward a sustainable society. Lactic acid, used for synthesizing the bioplastic polylactide, has been produced by microbial fermentation using primarily glucose. Lignocellulosic hydrolysates contain high concentrations of cellobiose and xylose. Here, we constructed a recombinant Saccharomyces cerevisiae strain capable of fermenting cellobiose and xylose into lactic acid. Specifically, genes (cdt-1, gh1-1, XYL1, XYL2, XYL3, and ldhA) coding for cellobiose transporter, -glucosidase, xylose reductase, xylitol dehydrogenase, xylulokinase, and lactate dehydrogenase were integrated into the S. cerevisiae chromosomes. The resulting strain produced lactic acid from cellobiose or xylose with high yields. When fermenting a cellulosic sugar mixture containing 10g/L glucose, 40g/L xylose, and 80g/L cellobiose, the engineered strain produced 83g/L of lactic acid with a yield of 0.66g lactic acid/g sugar (66% theoretical maximum). This study demonstrates initial steps toward the feasibility of sustainable production of lactic acid from lignocellulosic sugars by engineered yeast. Biotechnol. Bioeng. 2016;113: 1075-1083. (c) 2015 Wiley Periodicals, Inc. C1 [Turner, Timothy L.; Zhang, Guo-Chang; Oh, Eun Joong; Jin, Yong-Su] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL USA. [Turner, Timothy L.; Zhang, Guo-Chang; Oh, Eun Joong; Subramaniam, Vijay; Jin, Yong-Su] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Adiputra, Andrew; Subramaniam, Vimal] Univ Illinois, Dept Mol & Cellular Biol, Urbana, IL 61801 USA. [Skory, Christopher D.] USDA ARS, Natl Ctr Agr Utilizat Res, RPT, 1815 N Univ St, Peoria, IL 61604 USA. [Jang, Ji Yeon; Yu, Byung Jo; Park, In] Korea Inst Ind Technol KITECH, Res Inst Sustainable Mfg Syst, IT Convergence Mat R&D Grp, Cheonan, South Korea. RP Jin, YS (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL USA.; Jin, YS (reprint author), Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. EM ysjin@illinois.edu FU Agriculture and Food Research Initiative Competitive [2015-67011-22806] FX Contract grant sponsor: Agriculture and Food Research Initiative Competitive; Contract grant number: 2015-67011-22806 NR 42 TC 3 Z9 3 U1 15 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0006-3592 EI 1097-0290 J9 BIOTECHNOL BIOENG JI Biotechnol. Bioeng. PD MAY PY 2016 VL 113 IS 5 BP 1075 EP 1083 DI 10.1002/bit.25875 PG 9 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DI4NN UT WOS:000373476700016 PM 26524688 ER PT J AU DeGrandi-Hoffman, G Ahumada, F Zazueta, V Chambers, M Hidalgo, G deJong, EW AF DeGrandi-Hoffman, Gloria Ahumada, Fabiana Zazueta, Victor Chambers, Mona Hidalgo, Geoffrey deJong, Emily Watkins TI Population growth of Varroa destructor (Acari: Varroidae) in honey bee colonies is affected by the number of foragers with mites SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Migration; Population models; Population dynamics; Parasite dispersal ID APIS-MELLIFERA COLONIES; JACOBSONI OUD; REPRODUCTIVE-CYCLES; HYMENOPTERA APIDAE; NATURAL CONDITIONS; INVASION; BROOD; MESOSTIGMATA; PREFERENCES; ONTOGENY AB Varroa mites are a serious pest of honey bees and the leading cause of colony losses. Varroa have relatively low reproductive rates, so populations should not increase rapidly, but often they do. Other factors might contribute to the growth of varroa populations including mite migration into colonies on foragers from other hives. We measured the proportion of foragers carrying mites on their bodies while entering and leaving hives, and determined its relationship to the growth of varroa populations in those hives at two apiary sites. We also compared the estimates of mite population growth with predictions from a varroa population dynamics model that generates estimates of mite population growth based on mite reproduction. Samples of capped brood and adult bees indicated that the proportion of brood cells infested with mites and adult bees with phoretic mites was low through the summer but increased sharply in the fall especially at site 1. The frequency of capturing foragers with mites on their bodies while entering or leaving hives also increased in the fall. The growth of varroa populations at both sites was not significantly related to our colony estimates of successful mite reproduction, but instead to the total number of foragers with mites (entering and leaving the colony). There were more foragers with mites at site 1 than site 2, and mite populations at site 1 were larger especially in the fall. The model accurately estimated phoretic mite populations and infested brood cells until November when predictions were much lower than those measured in colonies. The rapid growth of mite populations particularly in the fall being a product of mite migration rather than mite reproduction only is discussed. C1 [DeGrandi-Hoffman, Gloria; Zazueta, Victor; Chambers, Mona; Hidalgo, Geoffrey; deJong, Emily Watkins] USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ USA. [Ahumada, Fabiana] AgSci Consulting LLC, Tucson, AZ USA. RP DeGrandi-Hoffman, G (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Rd, Tucson, AZ USA. EM Gloria.Hoffman@ARS.USDA.GOV FU USDA FX This project was funded by a USDA Areawide Grant to GD-H. The authors thank Drs. Robert Danka and Blair Christian for reviews of earlier versions of this manuscript and many helpful suggestions and additions. NR 32 TC 0 Z9 0 U1 3 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD MAY PY 2016 VL 69 IS 1 BP 21 EP 34 DI 10.1007/s10493-016-0022-9 PG 14 WC Entomology SC Entomology GA DI9YJ UT WOS:000373858300003 PM 26910522 ER PT J AU Burroughs, JE Thomasson, JA Marsella, R Greiner, EC Allan, SA AF Burroughs, Jennifer E. Thomasson, J. Alex Marsella, Rosanna Greiner, Ellis C. Allan, Sandra A. TI Ticks associated with domestic dogs and cats in Florida, USA SO EXPERIMENTAL AND APPLIED ACAROLOGY LA English DT Article DE Ticks; Dog; Cat; Rhipicephalus; Amblyomma; Ixodes; Florida ID WHITE-TAILED DEER; CYTAUXZOON-FELIS; INFECTIOUS-DISEASES; BORNE PATHOGENS; UNITED-STATES; IXODID TICKS; FERAL CATS; PREVALENCE; ACARI; SEROPREVALENCE AB Voluntary collections of ticks from domestic dogs and cats by veterinary practitioners across Florida, USA, were conducted over a 10 month period. Of the 1337 ticks submitted, five species of ixodid ticks were identified and included Rhipicephalus sanguineus, Amblyomma americanum, A. maculatum, Dermacentor variabilis, and Ixodes scapularis. Most ticks were collected from dogs (98.4 %) with the most predominant species being R. sanguineus (94.3 %). Of the ticks collected from cats (1.6 %), A. americanum were the most common (74 %). Only R. sanguineus were collected throughout the state, with the other species collected only in central and north Florida. The tick species collected from dogs and cats represent a risk to these domestic species as well as associated humans for a range of tick-borne diseases in Florida. C1 [Burroughs, Jennifer E.; Greiner, Ellis C.] Univ Florida, Coll Vet Med, Dept Infect Dis & Pathol, Gainesville, FL 32607 USA. [Thomasson, J. Alex] Merial Ltd, Field Vet Serv, Duluth, GA 30096 USA. [Marsella, Rosanna] Univ Florida, Coll Vet Med, Dept Small Anim Clin Sci, Gainesville, FL 32607 USA. [Allan, Sandra A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Burroughs, Jennifer E.] US Army, Med Command, Carlisle, PA 17013 USA. RP Allan, SA (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM sandy.allan@ars.usda.gov NR 50 TC 1 Z9 1 U1 10 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-8162 EI 1572-9702 J9 EXP APPL ACAROL JI Exp. Appl. Acarol. PD MAY PY 2016 VL 69 IS 1 BP 87 EP 95 DI 10.1007/s10493-016-0019-4 PG 9 WC Entomology SC Entomology GA DI9YJ UT WOS:000373858300008 PM 26888081 ER PT J AU Huang, JY Scudiero, E Bagtang, M Corwin, DL Triantafilis, J AF Huang, Jingyi Scudiero, Elia Bagtang, Michael Corwin, Dennis L. Triantafilis, John TI Monitoring scale-specific and temporal variation in electromagnetic conductivity images SO IRRIGATION SCIENCE LA English DT Article ID SOIL-WATER CONTENT; ELECTRICAL-CONDUCTIVITY; FIELD-SCALE; MULTIRESOLUTION ANALYSIS; SPATIAL VARIABILITY; WAVELET TRANSFORM; SALINITY; INDUCTION; LANDSCAPE; DIMENSIONS AB In semiarid and arid landscapes, irrigation sustains agricultural activity but because of increasing demands on water resources there is a need to make gains in efficiency. As such spatial variation of soil properties such as clay and salinity needs to be understood because they strongly influence soil moisture availability. One way is to use electromagnetic induction because apparent soil electrical conductivity (ECa) is related to volumetric soil moisture (theta), clay and salinity (ECe). However, depth-specific variation has not been explored. Our aim is to generate electromagnetic conductivity images (EMCIs) by inverting DUALEM-421 ECa and show how true electrical conductivity (sigma) can be correlated with theta, clay, ECe and bulk density (rho) on different days post-irrigation (i.e., 1, 4 and 12 days). Two-dimensional multi-resolution analysis (MRA) is used to show how spatio-temporal variation in sigma is scale-specific and how soil properties influence sigma at different scales. We study this beneath a pivot irrigated alfalfa crop. We found that sigma on days 1 and 4 was correlated with theta (Pearson's r = 0.79 and 0.61) and clay (0.86 and 0.80) and the dominant scale of variation occurred at 9.3-18.7 m (50.21 % of total variation), > 74.7 m (23.18 %) and 4.7-9.3 m (16.29 %). Between 9.3-18.7 and 4.7-9.3 m the variation may be a function of the cutter width (8 m), while > 74.7 m may be change in clay and ECe and gantry spacing (similar to 48 m). The sprinkler spacing (1.2 and 1.6 m) explains short-scale variation at 1.2-2.3 m. C1 [Huang, Jingyi; Triantafilis, John] UNSW Australia, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia. [Scudiero, Elia; Corwin, Dennis L.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. [Bagtang, Michael] Agr Waste Solut, Scott Bros Dairy, Moreno Valley, CA 92555 USA. RP Triantafilis, J (reprint author), UNSW Australia, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia. EM j.triantafilis@unsw.edu.au OI Scudiero, Elia/0000-0003-4944-721X; Huang, Jingyi/0000-0002-1209-9699 NR 47 TC 1 Z9 1 U1 4 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAY PY 2016 VL 34 IS 3 BP 187 EP 200 DI 10.1007/s00271-016-0496-6 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DI5WZ UT WOS:000373572500002 ER PT J AU Mounce, RB O'Shaughnessy, SA Blaser, BC Colaizzi, PD Evett, SR AF Mounce, Ryan B. O'Shaughnessy, Susan A. Blaser, Brock C. Colaizzi, Paul D. Evett, Steven R. TI Crop response of drought-tolerant and conventional maize hybrids in a semiarid environment SO IRRIGATION SCIENCE LA English DT Article ID WATER-USE EFFICIENCY; TEXAS HIGH-PLAINS; HARVEST INDEX; GRAIN-YIELD; DEFICIT IRRIGATION; CORN HYBRIDS; EVAPOTRANSPIRATION; STRESS; MANAGEMENT; COMPONENTS AB In the High Plains, corn (Zea mays L.) is an important commodity for livestock feed. However, limited water resources and drought conditions continue to hinder corn production. Drought-tolerant (DT) corn hybrids could help maintain high yields under water-limited conditions, though consistent response of such hybrids is unverified. In this two-year study, the effects of three irrigation treatments were investigated for a DT and conventional maize hybrid, Pioneer AQUAMax P0876HR and Pioneer 33Y75, respectively. In 2013, the drier of the 2 years, irrigation amounts and crop water use (ETc) were greater for the conventional hybrid, but grain water use efficiency (WUE) and harvest index were significantly greater for the DT hybrid. In 2014, grain yields and WUE were not significantly different between hybrids. However, irrigation amounts, ETc and biomass yields were greater for the conventional hybrid. Results from both years indicate that the DT hybrid required less water to maximize grain yield as compared to the conventional hybrid. Producing relatively high yields with reduced amounts of water may provide a means for producers to continue corn production in a semiarid environment with declining water supplies. C1 [Mounce, Ryan B.; Blaser, Brock C.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX USA. [O'Shaughnessy, Susan A.; Colaizzi, Paul D.; Evett, Steven R.] USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. RP O'Shaughnessy, SA (reprint author), USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. EM susan.oshaughnessy@ars.usda.gov NR 56 TC 0 Z9 0 U1 6 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAY PY 2016 VL 34 IS 3 BP 231 EP 244 DI 10.1007/s00271-016-0497-5 PG 14 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DI5WZ UT WOS:000373572500006 ER PT J AU McLaughlin, MR Brooks, JP Adeli, A Jenkins, JN AF McLaughlin, M. R. Brooks, J. P. Adeli, A. Jenkins, J. N. TI Improving estimates of N and P loads in irrigation water from swine manure lagoons SO IRRIGATION SCIENCE LA English DT Article ID WASTE-WATER; NUTRIENTS; EFFLUENT; EMISSIONS; BACTERIA; NITROGEN; AMMONIA AB The implementation of nutrient management plans for confined animal feeding operations requires recording N and P loads from land-applied manure, including nutrients applied in irrigation water from manure treatment lagoons. By regulation, lagoon irrigation water nutrient records in Mississippi must be based on at least one lagoon water nutrient analysis annually. Research in Mississippi has shown that N and P levels in lagoon water, and the N:P ratio, vary significantly through the year. Nutrient estimates based on one annual analysis do not account for this variability and may overestimate or underestimate N and P loads. The present study reports an improved method to more precisely estimate N and P loads in irrigation water from swine manure lagoons. The method is based on predictable annual cycles of N and P levels in lagoon water and employs simple curve-fitting of lagoon-specific formulas derived by analyses of historical data. Similarity of curves from analyses of Mississippi lagoons and other lagoon studies suggests that the method can be applied using the often limited nutrient data for a lagoon to more precisely estimate seasonal shifts of N and P and to improve the precision of estimates for N and P in irrigation water. Although the present study focused on swine manure lagoons in the southern US, recognition that the annual N cycle in lagoon water is temperature driven, suggests that additional research incorporating temperature into future models could extend these models to other types of waste treatment lagoons and climates. C1 [McLaughlin, M. R.; Brooks, J. P.; Adeli, A.; Jenkins, J. N.] ARS, Genet & Sustainable Agr Res Unit, USDA, POB 5367, Mississippi State, MS 39762 USA. RP McLaughlin, MR (reprint author), ARS, Genet & Sustainable Agr Res Unit, USDA, POB 5367, Mississippi State, MS 39762 USA. EM mike.mclaughlin@ars.usda.gov FU USDA-ARS CRIS Project [6406-21610-010-00D] FX This study was funded in-house by USDA-ARS CRIS Project 6406-21610-010-00D. NR 20 TC 0 Z9 0 U1 2 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAY PY 2016 VL 34 IS 3 BP 245 EP 260 DI 10.1007/s00271-016-0495-7 PG 16 WC Agronomy; Water Resources SC Agriculture; Water Resources GA DI5WZ UT WOS:000373572500007 ER PT J AU McCann, L Claassen, R AF McCann, Laura Claassen, Roger TI Farmer Transaction Costs of Participating in Federal Conservation Programs: Magnitudes and Determinants SO LAND ECONOMICS LA English DT Article ID ENVIRONMENTAL-POLICIES; MINNESOTA-RIVER; ADOPTION AB Transaction costs may be a barrier to participation in USDA conservation programs. Data on perceived barriers and transaction costs from the 2012 USDA Agricultural Resources Management Survey of soybean farmers were analyzed. Of farmers who had not applied for programs, almost a third agreed that applying for programs and documenting compliance (perceived transaction costs) were barriers to participation. The measured magnitudes of transaction costs of those who did apply varied by program but do not seem particularly onerous and are lower than in European studies. Regression analysis indicates that complexity of the program and the farming system may increase transaction costs. C1 [McCann, Laura] Univ Missouri, Dept Agr & Appl Econ, Columbia, MO 65211 USA. [Claassen, Roger] Econ Res Serv, USDA, Washington, DC USA. RP McCann, L (reprint author), Univ Missouri, Dept Agr & Appl Econ, Columbia, MO 65211 USA. FU Missouri Agricultural Experiment Station FX The views expressed are those of the authors and cannot be attributed to the U.S. Department of Agriculture or the Economic Research Service. We thank the NRCS-REAP staff for their assistance in devising the survey questions. The senior author acknowledges the support of the Missouri Agricultural Experiment Station. We also acknowledge the helpful comments of two reviewers. NR 38 TC 2 Z9 2 U1 6 U2 12 PU UNIV WISCONSIN PRESS PI MADISON PA JOURNAL DIVISION, 1930 MONROE ST, 3RD FL, MADISON, WI 53711 USA SN 0023-7639 EI 1543-8325 J9 LAND ECON JI Land Econ. PD MAY PY 2016 VL 92 IS 2 BP 256 EP 272 PG 17 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA DI3QJ UT WOS:000373413200004 ER PT J AU Anderson, KE Rodrigues, PAP Mott, BM Maes, P Corby-Harris, V AF Anderson, Kirk E. Rodrigues, Pedro A. P. Mott, Brendon M. Maes, Patrick Corby-Harris, Vanessa TI Ecological Succession in the Honey Bee Gut: Shift in Lactobacillus Strain Dominance During Early Adult Development SO MICROBIAL ECOLOGY LA English DT Article DE Gut microbiota; Lactobacillus firm 5; Supplemental nutrition; Social environment ID APIS-MELLIFERA L; BACTERIAL COMMUNITIES; MICROBIOTA; SEQUENCES; COLONIES; BEHAVIOR; CERANA; AGE AB In many vertebrates, social interactions and nutrition can affect the colonization of gut symbionts across generations. In the highly social honey bee, it is unknown to what extent the hive environment and older worker individuals contribute to the generational transmission of core gut bacteria. We used high-throughput sequencing to investigate the effect of nest materials and social contact on the colonization and succession of core hindgut microbiota in workers. With only brief exposure to hive materials following natural eclosion, gut bacterial communities at 3 and 7 days contained phylotypes typically found in the guts of mature adults regardless of treatment. Continuous exposure to nest materials or direct social interactions with mature adults did not affect the diversity or abundance of gut bacterial communities at the scale examined. Similarly, a common pollen supplement fed by beekeepers during pollen dearth had no effect. A consideration of unique OTUs revealed extensive microbial succession independent of treatment. The dominant Lactobacillus strain at 3 days was largely replaced by a different strain at day 7, revealing the colonization signature of a pioneer species. Similar but less pronounced patterns were evident in less abundant OTU's, many of which may influence community succession via alteration of the gut environment. Our results indicate that the process of bacterial community colonization in the hindgut is resilient to changes in the nutritional, hive, and social environment. Greater taxonomic resolution is needed to accurately resolve questions of ecological succession and typical proportional variation within and between core members of the gut bacterial community. C1 [Anderson, Kirk E.; Mott, Brendon M.; Corby-Harris, Vanessa] USDA ARS, Carl Hayden Bee Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. [Anderson, Kirk E.; Rodrigues, Pedro A. P.; Maes, Patrick; Corby-Harris, Vanessa] Univ Arizona, Grad Interdisciplinary Program Entomol & Insect S, Tucson, AZ 85721 USA. [Anderson, Kirk E.; Rodrigues, Pedro A. P.; Maes, Patrick; Corby-Harris, Vanessa] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. RP Anderson, KE (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA.; Anderson, KE (reprint author), Univ Arizona, Grad Interdisciplinary Program Entomol & Insect S, Tucson, AZ 85721 USA.; Anderson, KE (reprint author), Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. EM kirk.anderson@ars.usda.gov; par@email.arizona.edu; brendonmott@gmail.com; pmaes@email.arizona.edu; v.corbyharris@gmail.com OI Rodrigues, Pedro/0000-0002-3280-7576 NR 55 TC 4 Z9 4 U1 12 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0095-3628 EI 1432-184X J9 MICROB ECOL JI Microb. Ecol. PD MAY PY 2016 VL 71 IS 4 BP 1008 EP 1019 DI 10.1007/s00248-015-0716-2 PG 12 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA DI7LY UT WOS:000373683000019 PM 26687210 ER PT J AU McCullough, DG Poland, TM Lewis, PA AF McCullough, Deborah G. Poland, Therese M. Lewis, Phillip A. TI Lethal trap trees: a potential option for emerald ash borer (Agrilus planipennis Fairmaire) management SO PEST MANAGEMENT SCIENCE LA English DT Article DE emerald ash borer; Fraxinus; girdled trees; emamectin benzoate; bait and kill ID COLEOPTERA BUPRESTIDAE; URBAN FOREST; TEMPORAL DISTRIBUTION; EMAMECTIN BENZOATE; BEETLE COLEOPTERA; SPRUCE BEETLE; SCOLYTIDAE; INVASION; POPULATIONS; SPREAD AB BACKGROUNDEconomic and ecological impacts of ash (Fraxinus spp.) mortality resulting from emerald ash borer (EAB) (Agrilus planipennis Fairmaire) invasion are severe in forested, residential and urban areas. Management options include girdling ash trees to attract ovipositing adult beetles and then destroying infested trees before larvae develop or protecting ash with a highly effective, systemic emamectin benzoate insecticide. Injecting this insecticide and then girdling injected trees a few weeks later could effectively create lethal trap trees, similar to a bait-and-kill tactic, if girdling does not interfere with insecticide translocation. We compared EAB larval densities on girdled trees, trees injected with the emamectin benzoate insecticide, trees injected with the insecticide and then girdled 18-21 days later and untreated controls at multiple sites. RESULTSPretreatment larval densities did not differ among treatments. Current-year larval densities were higher on girdled and control trees than on any trees treated with insecticide at all sites. Foliar residue analysis and adult EAB bioassays showed that girdling trees after insecticide injections did not reduce insecticide translocation. CONCLUSIONSGirdling ash trees to attract adult EAB did not reduce efficacy of emamectin benzoate trunk injections applied 18days earlier and could potentially be used in integrated management programs to slow EAB population growth. (c) 2015 Society of Chemical Industry C1 [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Poland, Therese M.] US Forest Serv, USDA, Northern Res Stn, E Lansing, MI USA. [Lewis, Phillip A.] Anim & Plant Hlth Inspect Serv, USDA, Buzzards Bay, MA USA. RP McCullough, DG (reprint author), 243 Nat Sci Bldg,288 Farm Lane, E Lansing, MI USA. EM mccullo6@msu.edu FU USDA Forest Service, Northeastern Area, State and Private Forestry FX We thank Nicholas Gooch, Andrea Anulewicz, Jacob Bournay, Emily Pastula, Andrew Tluczek and James Wieferich, all of Michigan State University, for their assistance in the field and laboratory. Access to study sites was provided by Jackie Blanc, Eaton Co. Parks, Richard Bowles, DeWitt, Clinton Co., Bryan Van Patton, Genesee Co. Parks, and the Michigan Department of Natural Resources. The TREE-age (R) insecticide was provided courtesy of Joseph Doccola, ArborJet, Inc., and David Cox, Syngenta (R). Funding for this work was provided by the USDA Forest Service, Northeastern Area, State and Private Forestry. NR 52 TC 1 Z9 1 U1 16 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2016 VL 72 IS 5 BP 1023 EP 1030 DI 10.1002/ps.4083 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA DI6JM UT WOS:000373605000021 PM 26194342 ER PT J AU Zelinka, SL Glass, SV Jakes, JE Stone, DS AF Zelinka, Samuel L. Glass, Samuel V. Jakes, Joseph E. Stone, Donald S. TI A solution thermodynamics definition of the fiber saturation point and the derivation of a wood-water phase (state) diagram SO WOOD SCIENCE AND TECHNOLOGY LA English DT Article ID DIFFERENTIAL SCANNING CALORIMETRY; FURFURYLATED NORWAY SPRUCE; TIME-DOMAIN NMR; CELL-WALL; MAGNETIC-RESONANCE; MOISTURE-CONTENT; LOBLOLLY-PINE; HIGH VALUES; SORPTION; CELLULOSE AB The fiber saturation point (FSP) is an important concept in wood-moisture relations that differentiates between the states of water in wood and has been discussed in the literature for over 100 years. Despite its importance and extensive study, the exact theoretical definition of the FSP and the operational definition (the correct way to measure the FSP) are still debated because different methods give a wide range of values. In this paper, a theoretical definition of the FSP is presented based on solution thermodynamics that treats the FSP as a phase boundary. This thermodynamic interpretation allows FSP to be calculated from the chemical potentials of bound and free water as a function of moisture content, assuming that they are both known. Treating FSP as a phase boundary naturally lends itself to the construction of a phase diagram of water in wood. A preliminary phase diagram is constructed with previously published data, and the phase diagram is extended to a state diagram by adding data on the glass transition temperatures of the wood components. The thermodynamic interpretation and resulting state diagram represent a potential framework for understanding how wood modification may affect wood-moisture relations. C1 [Zelinka, Samuel L.; Glass, Samuel V.] US Forest Serv, Forest Prod Lab, Bldg & Fire Sci, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. [Jakes, Joseph E.] US Forest Serv, Forest Prod Lab, Forest Biopolymers Sci & Engn, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. [Stone, Donald S.] Univ Wisconsin, Mat Sci & Engn, 1509 Univ Ave, Madison, WI 53706 USA. RP Zelinka, SL (reprint author), US Forest Serv, Forest Prod Lab, Bldg & Fire Sci, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM szelinka@fs.fed.us OI Zelinka, Samuel/0000-0002-1348-7730 NR 68 TC 2 Z9 2 U1 4 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0043-7719 EI 1432-5225 J9 WOOD SCI TECHNOL JI Wood Sci. Technol. PD MAY PY 2016 VL 50 IS 3 BP 443 EP 462 DI 10.1007/s00226-015-0788-7 PG 20 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA DI8HY UT WOS:000373743300001 ER PT J AU Rohumaa, A Antikainen, T Hunt, CG Frihart, CR Hughes, M AF Rohumaa, Anti Antikainen, Toni Hunt, Christopher G. Frihart, Charles R. Hughes, Mark TI The influence of log soaking temperature on surface quality and integrity performance of birch (Betula pendula Roth) veneer SO WOOD SCIENCE AND TECHNOLOGY LA English DT Article ID PEELING PROCESS; WOOD SURFACES; ROUGHNESS; BEECH; MICROMECHANICS; FRACTURE; PLYWOOD AB Wood material surface properties play an important role in adhesive bond formation and performance. In the present study, a test method was developed to evaluate the integrity of the wood surface, and the results were used to understand bond performance. Materials used were rotary cut birch (Betula pendula Roth) veneers, produced from logs soaked at 20 or 70 A degrees C prior to peeling and followed by drying at 160 A degrees C. Surface quality was evaluated through surface roughness measurement, SEM imaging, lathe check depth evaluation and surface integrity testing. The results show that soaking logs at 70 A degrees C rather than at 20 A degrees C before peeling produced veneers with decreased surface roughness, shallower lathe checks and better integrity. All these parameters are mainly affected by the lathe check depth, which is observed on the loose side of veneer. On the tight side of veneer, the only detectable differences were the mode of cell wall failure observed under SEM, and the greater amount of very fine particles loosely bound to the surface. Higher soaking temperature also produced a surface with "hairy" structure and larger surface area, which could promote strong adhesive bond development. The surface integrity measurements were found to be very useful for evaluating the quality of veneer surfaces. C1 [Rohumaa, Anti; Antikainen, Toni; Hughes, Mark] Aalto Univ, Sch Chem Technol, Dept Forest Prod Technol, Vuorimiehentie 1, Espoo 02150, Finland. [Hunt, Christopher G.; Frihart, Charles R.] US Forest Serv, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Rohumaa, A (reprint author), Aalto Univ, Sch Chem Technol, Dept Forest Prod Technol, Vuorimiehentie 1, Espoo 02150, Finland. EM anti.rohumaa@aalto.fi RI Hughes, Mark/G-2249-2013; Hunt, Christopher/D-6339-2011 OI Hunt, Christopher/0000-0002-4799-7085 FU Finnish Funding Agency for Technology and Innovation (Tekes) and industry FX Authors would like to acknowledge the financial assistance provided by the Finnish Funding Agency for Technology and Innovation (Tekes) and industry. Warm thanks are extended to staff at the US Forest Service, Forest Product Laboratory in Madison. NR 23 TC 1 Z9 1 U1 2 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0043-7719 EI 1432-5225 J9 WOOD SCI TECHNOL JI Wood Sci. Technol. PD MAY PY 2016 VL 50 IS 3 BP 463 EP 474 DI 10.1007/s00226-016-0805-5 PG 12 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA DI8HY UT WOS:000373743300002 ER PT J AU LaFrentz, BR Lozano, CA Shoemaker, CA Garcia, JC Xu, DH Lovoll, M Rye, M AF LaFrentz, Benjamin R. Lozano, Carlos A. Shoemaker, Craig A. Garcia, Julio C. Xu, De-Hai Lovoll, Marie Rye, Morten TI Controlled challenge experiment demonstrates substantial additive genetic variation in resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae SO AQUACULTURE LA English DT Article DE Streptococcus iniae; Nile tilapia; Oreochromis niloticus; Selective breeding; Disease resistance; Heritability ID COLD-WATER DISEASE; SALMON SALMO-SALAR; RAINBOW-TROUT; ATLANTIC SALMON; SURVIVAL; VACCINE; GROWTH; PERFORMANCE; IMPROVEMENT; MORTALITY AB Streptococcus iniae is an etiologic agent of streptococcal disease in tilapia and is one of several Streptococcus spp. that negatively impact worldwide tilapia production. Methods for the prevention and control of S. iniae include vaccines, management strategies, and antibiotics. A complimentary preventative approach may include selective breeding for disease resistance, but the potential for this is unknown in tilapia. This study was initiated to challenge Nile tilapia (Oreochromis niloticus) families for estimation of genetic parameters for resistance to S. iniae. A total of 143 full-and paternal half-sib families (avg. 176 g, sd = 50 g) were divided into two groups with each containing on average 9 fish per family. The challenge was designed with the intent to utilize fish injected with S. iniae (Group 1) as shedder fish to transfer the bacterium to cohabitated fish (Group 2). Tilapia from Group 1 were challenged by intraperitoneal injection with a volume containing 1.15 x 10(8) colonyforming units S. iniae per fish, and then cohabitated with tilapia from Group 2 in a single tank. Accumulated mortality at the end of the experiment was 60% for the fish challenged by injection and 6.4% for fish challenged by cohabitation. The results revealed high variation for mean survival of the families injected with S. iniae (range from 0% to 100%, CV 69%). The estimated heritability of post-challenge survival in Group 1 was 0.42 +/- 0.07 on the observed binary scale and 0.58 +/- 0.09 on the underlying liability scale, derived from fitting a linear animal model and a sire-dam threshold model, respectively. In summary, substantial additive genetic variation in resistance to S. iniae was observed when fish were challenged by injection, and this suggests promise for genetic improvement of tilapia for resistance to S. iniae through selective breeding. Published by Elsevier B.V. C1 [LaFrentz, Benjamin R.; Shoemaker, Craig A.; Garcia, Julio C.; Xu, De-Hai] USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. [Lozano, Carlos A.; Rye, Morten] Akvaforsk Genet Ctr AS, Sjolsengvegen 22, N-6600 Sunndalsora, Norway. [Lovoll, Marie] VESO Vikean, Beisvagveien 107, N-7810 Vikan, Namsos, Norway. RP LaFrentz, BR (reprint author), USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM benjamin.lafrentz@ars.usda.gov FU USDA-ARS CRIS [6010-32000-026-00D] FX The authors acknowledge Spring Genetics for providing the Nile tilapia under a Material Transfer Agreement for this research and for the technical assistance of Jose Ospina and Hideyoshi Segovia Uno. The authors also acknowledge the technical support of Paige Mumma, Curtis Day, Ning Qin, and Jana Mladek of USDA-ARS during the challenge test trials conducted in Auburn. This research was supported by USDA-ARS CRIS Project No. 6010-32000-026-00D, Pathogen characterization, host immune response and development of strategies to reduce losses to disease in aquaculture. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 29 TC 2 Z9 2 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD MAY 1 PY 2016 VL 458 BP 134 EP 139 DI 10.1016/j.aquaculture.2016.02.034 PG 6 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DI3CL UT WOS:000373375700016 ER PT J AU Chavez, E He, ZL Stoffella, PJ Mylavarapu, RS Li, YC Baligar, VC AF Chavez, E. He, Z. L. Stoffella, P. J. Mylavarapu, R. S. Li, Y. C. Baligar, V. C. TI Chemical speciation of cadmium: An approach to evaluate plant-available cadmium in Ecuadorian soils under cacao production SO CHEMOSPHERE LA English DT Article DE Cadmium; Ecuador; Cacao; Fractionation; Available Cd; Soil contamination ID SEQUENTIAL EXTRACTION PROCEDURE; HEAVY-METALS; CONTAMINATED SOILS; TRACE-ELEMENTS; DIFFERENT RICE; CD; FRACTIONATION; ZN; ACCUMULATION; SINGLE AB Elevated concentration of cadmium (Cd) in cacao beans has raised serious concerns about the chocolate consumption on human health. Accumulation of Cd in cacao bean in southern Ecuador has been related to soil contamination. In this study, soil fractionation approach was used to identify available Cd pools in the soils and to correlate these Cd pools with bean Cd concentration and soil test indexes. The distribution of soil Cd fractions decreased in the order: oxidizable > acid-soluble > residual > reducible >> water-soluble (+exchangeable). Oxidizable and acid-soluble fractions accounted for 59 and 68% of the total recoverable Cd for the 0-5 and 5-15 cm soil depth, respectively. Acid-soluble fraction was closely related to bean-Cd, with correlation coefficients (r) of 0.70 and 0.81 (P < 0.01) for the 0-5 and 5-15 cm soil depth, respectively. Acid-soluble Cd was significantly correlated with 0.01 M HCl- (r = 0.99, P < 0.01) or Mehlich 3- extractable Cd (r = 0.97, P < 0.01). These results indicate that acid-soluble Cd fraction is an important part of available Cd pool. Since approximately 60% of Cd in the cacao-growing soils is related to the acid-soluble fraction and bound to organic matter, remediation of the contaminated soils should consider to the dynamics of soil pH and organic matter content. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chavez, E.; He, Z. L.; Stoffella, P. J.] Univ Florida, Inst Food & Agr Sci, Indian River Res & Educ Ctr, 2199 South Rock Rd, Ft Pierce, FL 34945 USA. [Mylavarapu, R. S.] Univ Florida, Inst Food & Agr Sci, Soil & Water Sci Dept, 171 McCarty Hall, Gainesville, FL 33611 USA. [Li, Y. C.] Univ Florida, Inst Food & Agr Sci, Trop Res & Educ Ctr, 18905 SW 280 St, Homestead, FL 33031 USA. [Chavez, E.] Escuela Super Politecn Litoral, Fac Ciencias Vida, Ctr Invest Biotecnol Ecuador, Km 30-5 Via Perimetral, Guayaquil, Guayas, Ecuador. [Baligar, V. C.] ARS, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP He, ZL (reprint author), Univ Florida, Inst Food & Agr Sci, Indian River Res & Educ Ctr, 2199 South Rock Rd, Ft Pierce, FL 34945 USA. EM zhe@ufl.edu RI He, Zhenli/R-1494-2016 OI He, Zhenli/0000-0001-7761-2070 FU Indian River Research and Education Center FX The Authors would like to thank the Secretary of Science and Technology of Ecuador and the Indian River Research and Education Center for the financial aid, we want to also express our gratitude to the Biotechnological Research Center of Ecuador (CIBE) for their assistance with sample collection and Brian Cain for chemical analysis of the samples. NR 33 TC 0 Z9 0 U1 18 U2 46 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD MAY PY 2016 VL 150 BP 57 EP 62 DI 10.1016/j.chemosphere.2016.02.013 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH4OK UT WOS:000372765100008 PM 26891357 ER PT J AU El Houssami, M Mueller, E Thomas, JC Simeoni, A Filkov, A Skowronski, N Gallagher, MR Clark, K Kremens, R AF El Houssami, Mohamad Mueller, Eric Thomas, Jan C. Simeoni, Albert Filkov, Alexander Skowronski, Nicholas Gallagher, Michael R. Clark, Kenneth Kremens, Robert TI Experimental Procedures Characterising Firebrand Generation in Wildland Fires SO FIRE TECHNOLOGY LA English DT Article DE Firebrands; Generation; Pine forest ID FUEL BEDS; IGNITION AB This study aims to develop a series of robust and efficient methodologies, which can be applied to understand and estimate firebrand generation and to evaluate firebrand showers close to a fire front. A field scale high intensity prescribed fire was conducted in the New Jersey Pine Barrens in March 2013. Vegetation was characterised with field and remotely sensed data, fire spread and intensity was characterised and meteorological conditions were monitored before and during the burn. Firebrands were collected from different locations in the forest and analysed for mass and size distribution. The majority were found to be bark slices (more than 70%) with substantial amounts of pine and shrub twigs. Shrub layer consumption was evaluated to supplement the firebrand generation study. Bark consumption was studied by measuring the circumference variation at several heights on each of three different pine trees. The variation was in the same order of magnitude as the bark thickness (1-5 mm). Testing and improving the protocol can facilitate the collection of compatible data in a wide range of ecosystems and fire environments, aiding in the development of solutions to prevent structural ignition at the Wildland Urban Interface. C1 [El Houssami, Mohamad; Mueller, Eric; Thomas, Jan C.; Simeoni, Albert] Univ Edinburgh, BRE Ctr Fire Safety Engn, Edinburgh EH9 3JL, Midlothian, Scotland. [Filkov, Alexander] Natl Res Tomsk State Univ, Tomsk 634050, Russia. [Skowronski, Nicholas] USDA Forest Serv, No Res Stn, Morgantown, WV 26505 USA. [Gallagher, Michael R.; Clark, Kenneth] USDA Forest Serv, No Res Stn, New Lisbon, NJ 08064 USA. [Kremens, Robert] Rochester Inst Technol, Rochester, NY 14623 USA. RP El Houssami, M (reprint author), Univ Edinburgh, BRE Ctr Fire Safety Engn, Edinburgh EH9 3JL, Midlothian, Scotland. EM m.elhoussami@ed.ac.uk RI Simeoni, Albert/B-4428-2011 OI Simeoni, Albert/0000-0002-5497-3794 FU Joint Fire Science Program [12-1-03-11]; Fulbright program of Institute of International Education; Government Assignment in the Field of Scientific Activity [1624]; Russian Federation [SP-3968.2013.1] FX Authors thank the New Jersey Forest Fire Service. The field experiment was supported by the Joint Fire Science Program (Project 12-1-03-11). Dr. Filkov was supported by the Fulbright program of Institute of International Education, the Government Assignment in the Field of Scientific Activity (Number 1624) and by the President of the Russian Federation Scholarship (Number SP-3968.2013.1). NR 34 TC 4 Z9 4 U1 3 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0015-2684 EI 1572-8099 J9 FIRE TECHNOL JI Fire Technol. PD MAY PY 2016 VL 52 IS 3 BP 731 EP 751 DI 10.1007/s10694-015-0492-z PG 21 WC Engineering, Multidisciplinary; Materials Science, Multidisciplinary SC Engineering; Materials Science GA DH9XB UT WOS:000373148200008 ER PT J AU Finney, MA McAllister, SS Maynard, TB Grob, IJ AF Finney, Mark A. McAllister, Sara S. Maynard, Trevor B. Grob, Ian J. TI A Study of Wildfire Ignition by Rifle Bullets SO FIRE TECHNOLOGY LA English DT Article DE Ignition; Hot particles; Rifle bullets; Ballistic impact; Smoldering ID FUEL BEDS; DEFORMATION; IMPACT AB Experiments were conducted to examine the potential for rifle bullets to ignite organic matter after impacting a hard surface. The tests were performed using a variety of common cartridges (7.62 x 51 [.308 Winchester (The use of tradenames is provided for informational purposes only and does not constitute an endorsement by the U.S. Department of Agriculture.)], 7.62 x 39, 7.62 x 54R, and 5.56 x 45 [.223 Remington]) and bullet materials (steel core, lead core, solid copper, steel jacket, and copper jacket). Bullets were fired at a steel plate that deflected fragments downward into a collection box containing oven-dried peat moss. We found that bullets could reliably cause ignitions, specifically those containing steel components (core or jacket) and those made of solid copper. Lead core-copper jacketed bullets caused one ignition in these tests. Thermal infra-red video and temperature sensitive paints suggested that the temperature of bullet fragments could exceed 800A degrees C. Bullet fragments collected from a water tank were larger for solid copper and steel core/jacketed bullets than for lead core bullets, which also facilitate ignition. Physical processes are reviewed with the conclusion that kinetic energy of bullets is transformed to thermal energy by plastic deformation and fracturing of bullets because of the high-strain rates during impact. Fragments cool rapidly but can ignite organic matter, particularly fine material, if very dry and close to the impact site. C1 [Finney, Mark A.; McAllister, Sara S.] USDA, Forest Serv, Missoula Fire Sci Lab, 5775 W US Highway 10, Missoula, MT 59808 USA. [Maynard, Trevor B.] USDA, Forest Serv, San Dimas Technol & Dev Ctr, San Dimas, CA USA. [Grob, Ian J.] USDA, Forest Serv, Missoula Technol & Dev Ctr, Missoula, MT USA. RP McAllister, SS (reprint author), USDA, Forest Serv, Missoula Fire Sci Lab, 5775 W US Highway 10, Missoula, MT 59808 USA. EM smcallister@fs.fed.us NR 36 TC 3 Z9 3 U1 1 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0015-2684 EI 1572-8099 J9 FIRE TECHNOL JI Fire Technol. PD MAY PY 2016 VL 52 IS 3 BP 931 EP 954 DI 10.1007/s10694-015-0518-6 PG 24 WC Engineering, Multidisciplinary; Materials Science, Multidisciplinary SC Engineering; Materials Science GA DH9XB UT WOS:000373148200017 ER PT J AU Brown, AW Stegelmeier, BL Colegate, SM Gardner, DR Panter, KE Knoppel, EL Hall, JO AF Brown, Ammon W. Stegelmeier, Bryan L. Colegate, Steven M. Gardner, Dale R. Panter, Kip E. Knoppel, Edward L. Hall, Jeffery O. TI The comparative toxicity of a reduced, crude comfrey (Symphytum officinale) alkaloid extract and the pure, comfrey-derived pyrrolizidine alkaloids, lycopsamine and intermedine in chicks (Gallus gallus domesticus) SO JOURNAL OF APPLIED TOXICOLOGY LA English DT Article DE comfrey; lycopsamine; intermedine; pyrrolizidine alkaloid; N-oxide; tisane ID LIVER-TISSUE; METABOLITES; RATS; INDUCTION AB Comfrey (Symphytum officinale), a commonly used herb, contains dehydropyrrolizidine alkaloids that, as a group of bioactive metabolites, are potentially hepatotoxic, pneumotoxic, genotoxic and carcinogenic. Consequently, regulatory agencies and international health organizations have recommended comfrey be used for external use only. However, in many locations comfrey continues to be ingested as a tisane or as a leafy vegetable. The objective of this work was to compare the toxicity of a crude, reduced comfrey alkaloid extract to purified lycopsamine and intermedine that are major constituents of S. officinale. Male, California White chicks were orally exposed to daily doses of 0.04, 0.13, 0.26, 0.52 and 1.04 mmol lycopsamine, intermedine or reduced comfrey extract per kg bodyweight (BW) for 10 days. After another 7 days chicks were euthanized. Based on clinical signs of poisoning, serum biochemistry, and histopathological analysis the reduced comfrey extract was more toxic than lycopsamine and intermedine. This work suggests a greater than additive effect of the individual alkaloids and/or a more potent toxicity of the acetylated derivatives in the reduced comfrey extract. It also suggests that safety recommendations based on purified compounds may underestimate the potential toxicity of comfrey. Published 2015. This article has been contributed to by US Government employees and their work is in the public domain in the USA. C1 [Brown, Ammon W.; Stegelmeier, Bryan L.; Colegate, Steven M.; Gardner, Dale R.; Panter, Kip E.; Knoppel, Edward L.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. [Colegate, Steven M.] Utah State Univ, Anim Dairy & Vet Sci, Logan, UT 84341 USA. [Hall, Jeffery O.] Utah State Univ, Utah Vet Diagnost Lab, Logan, UT 84341 USA. RP Brown, AW (reprint author), USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. EM brown.ammon@gmail.com FU USDA Agriculture Research Service [NP215-2080-32630-012-00] FX The authors would like to thank Joseph Jacobsen for histology support. This work was funded through the USDA Agriculture Research Service NP215-2080-32630-012-00. NR 28 TC 1 Z9 1 U1 6 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0260-437X EI 1099-1263 J9 J APPL TOXICOL JI J. Appl. Toxicol. PD MAY PY 2016 VL 36 IS 5 BP 716 EP 725 DI 10.1002/jat.3205 PG 10 WC Toxicology SC Toxicology GA DH9QF UT WOS:000373130100008 PM 26177929 ER PT J AU Stapanian, MA Schumacher, W Gara, B Monteith, SE AF Stapanian, Martin A. Schumacher, William Gara, Brian Monteith, Steven E. TI Negative effects of excessive soil phosphorus on floristic quality in Ohio wetlands SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Wetland; Mehlich-3 phosphorus; Soil; Vegetation index of biological integrity; Rapid assessment method ID AGRICULTURAL WATERSHEDS; RIPARIAN WETLANDS; VEGETATION INDEX; BIOTIC INTEGRITY; WASTE-WATER; RUNOFF; DISTURBANCE; INVASION; LAND; DENITRIFICATION AB Excessive soil nutrients, often from agricultural runoff, have been shown to negatively impact some aspects of wetland plant communities. We measured plant-available phosphorus (Mehlich-3: MeP) in soil samples, and assessed the vascular plant community and habitat degradation at 27 emergent and 13 forested wetlands in Ohio, USA. We tested two hypotheses: (1) that an index of vegetation biological integrity based on floristic quality was lower in wetlands with higher concentrations of MeP in the soil, and (2) that higher concentrations of MeP occurred in wetlands with more habitat degradation (i.e., lower quality), as estimated by a rapid assessment method. Hypothesis (1) was supported for emergent, but not for forested wetlands. Hypothesis (2) was marginally supported (P = 0.09) for emergent, but not supported for forested wetlands. The results indicate that the effect of concentration of phosphorus in wetland soils and the quality of plant species assemblages in wetlands is more complex than shown in site-specific studies and may depend in part on degree of disturbance in the surrounding watershed and dominant wetland vegetation type. Woody plants in forested wetlands are typically longer lived than herbaceous species in the understory and emergent wetlands, and may persist despite high inputs of phosphorus. Further, the forested wetlands were typically surrounded by a wide band of forest vegetation, which may provide a barrier against sedimentation and the associated phosphorus inputs to the wetland interior. Our results indicate that inferences about soil nutrient conditions made from rapid assessment methods for assessing wetland habitat condition may not be reliable. Published by Elsevier B.V. C1 [Stapanian, Martin A.] US Geol Survey, Great Lakes Sci Ctr, Lake Erie Biol Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. [Schumacher, William; Gara, Brian] Ohio Environm Protect Agcy, 4675 Homer Ohio Lane, Groveport, OH 43125 USA. [Monteith, Steven E.] Nat Resource Conservat Serv, USDA, 100 Centennial Mall North, Lincoln, NE 66508 USA. RP Stapanian, MA (reprint author), US Geol Survey, Great Lakes Sci Ctr, Lake Erie Biol Stn, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM mstapanian@usgs.gov OI Stapanian, Martin/0000-0001-8173-4273 NR 54 TC 2 Z9 2 U1 17 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 1 PY 2016 VL 551 BP 556 EP 562 DI 10.1016/j.scitotenv.2016.02.041 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH2BT UT WOS:000372589800058 PM 26896584 ER PT J AU Fairbairn, DJ Karpuzcu, ME Arnold, WA Barber, BL Kaufenberg, EF Koskinen, WC Novak, PJ Rice, PJ Swackhamer, DL AF Fairbairn, David J. Karpuzcu, M. Ekrem Arnold, William A. Barber, Brian L. Kaufenberg, Elizabeth F. Koskinen, William C. Novak, Paige J. Rice, Pamela J. Swackhamer, Deborah L. TI Sources and transport of contaminants of emerging concern: A two-year study of occurrence and spatiotemporal variation in a mixed land use watershed SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Pharmaceutical personal care product (PPCP); Pesticide; Fate transport; Agricultural; Urban; Endocrine disrupting compound (EDC) ID PERSONAL CARE PRODUCTS; ENDOCRINE DISRUPTING COMPOUNDS; WASTE-WATER; SURFACE-WATER; DRINKING-WATER; ORGANIC CONTAMINANTS; ANTHROPOGENIC MARKER; SEASONAL-VARIATION; TREATMENT PLANTS; RIVER-BASIN AB The occurrence and spatiotemporal variation of 26 contaminants of emerging concern (CECs) were evaluated in 68 water samples in 2011-2012 in the Zumbro River watershed, Minnesota, U.S.A. Samples were collected across a range of seasonal/hydrological conditions from four stream sites that varied in associated land use and presence of an upstream wastewater treatment plant (WWTP). Selected CECs included human/veterinary pharmaceuticals, personal care products, pesticides, phytoestrogens, and commercial/industrial compounds. Detection frequencies and concentrations varied, with atrazine, metolachlor, acetaminophen, caffeine, DEET, and trimethoprim detected in more than 70% of samples, acetochlor, mecoprop, carbamazepine, and daidzein detected in 30%-50% of samples, and 4-nonylphenol, cotinine, sulfamethoxazole, erythromycin, tylosin, and carbaryl detected in 10%-30% of samples. The remaining target CECs were not detected in water samples. Three land use-associated trends were observed for the detected CECs. Carbamazepine, 4-nonylphenol, erythromycin, sulfamethoxazole, tylosin, and carbaryl profiles were WWTP-dominated, as demonstrated by more consistent loading and significantly greater concentrations downstream of the WWTP and during low-flow seasons. In contrast, acetaminophen, trimethoprim, DEET, caffeine, cotinine, and mecoprop patterns demonstrated both seasonally-variable non-WWTP-associated and continual WWTP-associated influences. Surface water studies of CECs often target areas near WWTPs. This study suggests that several CECs often characterized as effluent-associated have additional important sources such as septic systems or land-applied biosolids. Finally, agricultural herbicide ( atrazine, acetochlor, and metolachlor) profiles were strongly influenced by agricultural land use and seasonal application-runoff, evident by significantly greater concentrations and loadings at upstream sites and in early summer when application and precipitation rates are greatest. Our results indicate that CEC monitoring studies should consider a range of land uses, seasonality, and transport pathways in relation to concentrations and loadings. This knowledge can augment CEC monitoring programs to result in more accurate source, occurrence, and ecological risk characterizations, more precisely targeted mitigation initiatives, and ultimately, enhanced environmental decision-making. (C) 2016 Elsevier B.V. All rights reserved. C1 [Fairbairn, David J.; Karpuzcu, M. Ekrem; Kaufenberg, Elizabeth F.; Swackhamer, Deborah L.] Univ Minnesota, Water Resources Ctr, 1985 Buford Ave, St Paul, MN 55108 USA. [Arnold, William A.; Novak, Paige J.] Univ Minnesota, Civil Environm & Geoengn, 500 Pillsbury Dr SE, Minneapolis, MN 55455 USA. [Barber, Brian L.] Univ Minnesota, Dept Soil Water & Climate, 1902 Dudley Ave, St Paul, MN 55108 USA. [Koskinen, William C.; Rice, Pamela J.] Univ Minnesota, USDA, ARS, 1991 Upper Buford Circle, St Paul, MN 55108 USA. [Kaufenberg, Elizabeth F.] Minnesota Pollut Control Agcy, 520 Lafayette Rd, St Paul, MN 55155 USA. [Karpuzcu, M. Ekrem] Istanbul Tech Univ, Fac Civil Engn, Dept Environm Engn, TR-34469 Istanbul, Turkey. RP Fairbairn, DJ (reprint author), Minnesota Pollut Control Agcy, 520 Lafayette Rd, St Paul, MN 55155 USA. EM fairb109@umn.edu OI Karpuzcu, Mahmut Ekrem/0000-0002-6245-9621 FU Minnesota Environment and Natural Resources Trust Fund [012-A2] FX This work was supported by the Minnesota Environment and Natural Resources Trust Fund (012-A2) as recommended by the Legislative-Citizen Commission on Minnesota Resources. We thank McGhie Betts, Inc. (Rochester, MN) for the technical support on watershed characterization; Dr. Megan Kelly(Water Resources Science, University of Minnesota) for her assistance with sampling and sample analysis; and Dr. Gary Oehlert (Department of Statistics, University of Minnesota) for his advice on statistical design and analysis. NR 65 TC 4 Z9 4 U1 41 U2 101 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD MAY 1 PY 2016 VL 551 BP 605 EP 613 DI 10.1016/j.scitotenv.2016.02.056 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH2BT UT WOS:000372589800063 PM 26897403 ER PT J AU Zhang, XC Zhang, GH Liu, BL Liu, B AF Zhang, X. C. (John) Zhang, G. H. Liu, B. L. Liu, B. TI Using cesium-137 to quantify sediment source contribution and uncertainty in a small watershed SO CATENA LA English DT Article DE Sediment tracer; Cesium-137; Sediment source fingerprinting; Mixing model; Uncertainty analysis ID FALLOUT RADIONUCLIDE TRACERS; SOUTH-EASTERN AUSTRALIA; SUSPENDED SEDIMENT; SOIL-EROSION; AGRICULTURAL CATCHMENT; GULLIED CATCHMENT; RIVER-BASINS; PB-210; CS-137; WILDFIRE AB Knowledge of sediment provenance is critical for precision conservation planning and calibration of soil erosion models. The objectives are to evaluate the ability of Cs-137 to apportion sediment source contributions, quantify uncertainty of the estimates, and estimate desirable sample numbers. We collected 50 surface soil samples from overland in a watershed (15.6 km(2)), 28 subsoil samples from gully bank, and 43 sediment samples in channels. The Cs-137 activity was measured by gamma spectrometry. Proportion means were calculated by solving a linear mixing model. Uncertainty was estimated by the first-order approximation and Monte Carlo (MC) simulation. The Cs-137 tracer was conservative in the fraction of <63 mu m. The Cs-137 activities differed between sources in the whole watershed at P = 0.05. The mean proportions of the <63 mu m fraction (relative error) predicted for the watershed using the mixing model were 0.42 (35%) for the overland source and 0.58 (25%) for the gully source, with a 95% confidence interval of +/- 0.145 for both. Overland source contributed most uncertainty to the proportion estimates, followed by sediment, with minimum from gully. The MC simulation predicted the same mean proportions, but with relative errors being <3% for both. Compared with the first-order approximation, MC underestimated uncertainty of the means due to the large sample number used in simulation. In general, 30 to 50 samples are needed for each source and sediment to generate reliable estimates using Cs-137. Estimated source proportions of the fine sediment may be converted to proportional contributions of total soil erosion by adjusting by particle sizes and sediment delivery ratio. No adjustment or weighting should be made directly to the mixing model unless conservativeness of the tracer is violated. Published by Elsevier B.V. C1 [Zhang, G. H.] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Zhang, X. C. (John)] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. [Liu, B. L.] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou, Gansu, Peoples R China. [Liu, B.] China Inst Water Resources & Hydropower Res, State Key Lab Simulat & Regulat Water Cycle River, Beijing, Peoples R China. [Zhang, X. C. (John)] 7207W Cheyenne St, El Reno, OK 73036 USA. [Zhang, G. H.] 19 Xinwai St, Beijing 100875, Peoples R China. [Liu, B. L.] 320 Donggangxi Rd, Lanzhou 730000, Gansu, Peoples R China. [Liu, B.] Chegongzhang Xilu 20,Nishasuo Dong Room 111, Beijing 100048, Peoples R China. RP Zhang, XC (reprint author), USDA ARS, Grazinglands Res Lab, El Reno, OK USA. EM john.zhang@ars.usda.gov FU State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University [2015-KF-09] FX This work was partially supported by State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, under the project number of 2015-KF-09. NR 62 TC 2 Z9 2 U1 4 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD MAY PY 2016 VL 140 BP 116 EP 124 DI 10.1016/j.catena.2016.01.021 PG 9 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DG9FB UT WOS:000372387100013 ER PT J AU Elser, JL Anderson, A Lindell, CA Dalsted, N Bernasek, A Shwiff, SA AF Elser, J. L. Anderson, A. Lindell, C. A. Dalsted, N. Bernasek, A. Shwiff, S. A. TI Economic impacts of bird damage and management in US sweet cherry production SO CROP PROTECTION LA English DT Article DE Bird damage; Pest management; Sweet cherries; Partial equilibrium; Welfare ID COST AB Bird damage is a common and costly problem for fruit producers, who try to limit damage by using management techniques. This analysis used survey data from producers in five U.S. states to estimate bird damage to sweet cherry (Prunus avium) crops with and without the use of bird management. A partial equilibrium model was applied to the data to estimate the change in the marginal cost of production resulting from disuse of bird management. The model incorporates both decreased yield and elimination of management costs. A welfare analysis was conducted with short and long run supply elasticities derived from time-series data using geometric distributed lags. With no bird management, total surplus in the United States decreases by about $185 to $238 million in the short run and $21 to $29 million in the long run, indicating that bird management has a large impact on cherry production and associated market outcomes, including price and consumption. Published by Elsevier Ltd. C1 [Elser, J. L.; Anderson, A.; Shwiff, S. A.] USDA APHIS WS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Lindell, C. A.] Michigan State Univ, Dept Integrat Biol, Ctr Global Change & Earth Observat, 1405 S Harrison Rd, E Lansing, MI 48823 USA. [Dalsted, N.] Colorado State Univ, Dept Agr & Resource Econ, 1172 Campus Delivery, Ft Collins, CO 80523 USA. [Bernasek, A.] Colorado State Univ, Dept Econ, 1771 Campus Delivery, Ft Collins, CO 80523 USA. RP Elser, JL (reprint author), USDA APHIS WS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Julie.L.Elser@aphis.usda.gov FU Specialty Crop Research Initiative of the National Institute of Food and Agriculture (NIFA Award) [2011-51181-30860] FX Funding was provided by the Specialty Crop Research Initiative of the National Institute of Food and Agriculture (NIFA Award No. 2011-51181-30860). The authors would like to thank Karen Moxcey for her contributions to the survey data analysis, as well as two anonymous reviewers whose comments helped improve the manuscript. NR 32 TC 0 Z9 0 U1 2 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD MAY PY 2016 VL 83 BP 9 EP 14 DI 10.1016/j.cropro.2016.01.014 PG 6 WC Agronomy SC Agriculture GA DH1PL UT WOS:000372557300002 ER PT J AU Hewitt, RE Bennett, AP Breen, AL Hollingsworth, TN Taylor, DL Chapin, FS Rupp, TS AF Hewitt, Rebecca E. Bennett, Alec P. Breen, Amy L. Hollingsworth, Teresa N. Taylor, D. Lee Chapin, F. Stuart, III Rupp, T. Scott TI Getting to the root of the matter: landscape implications of plant-fungal interactions for tree migration in Alaska SO LANDSCAPE ECOLOGY LA English DT Article DE Alaska; ALFRESCO; Climate change; Ectomycorrhizal fungi; Treeline; Wildfire ID CLIMATE-CHANGE; ECTOMYCORRHIZAL FUNGI; BOREAL FOREST; INTERIOR ALASKA; NORTHERN ALASKA; VEGETATION-RESPONSE; ECOSYSTEM MODELS; WHITE SPRUCE; FIRE; SOIL AB Forecasting the expansion of forest into Alaska tundra is critical to predicting regional ecosystem services, including climate feedbacks such as carbon storage. Controls over seedling establishment govern forest development and migration potential. Ectomycorrhizal fungi (EMF), obligate symbionts of all Alaskan tree species, are particularly important to seedling establishment, yet their significance to landscape vegetation change is largely unknown. We used ALFRESCO, a landscape model of wildfire and vegetation dynamics, to explore whether EMF inoculum potential influences patterns of tundra afforestation and associated flammability. Using two downscaled CMIP3 general circulation models (ECHAM5 and CCCMA) and a mid-range emissions scenario (A1B) at a 1 km(2) resolution, we compared simulated tundra afforestation rates and flammability from four parameterizations of EMF effects on seedling establishment and growth from 2000 to 2100. Modeling predicted an 8.8-18.2 % increase in forest cover from 2000 to 2100. Simulations that explicitly represented landscape variability in EMF inoculum potential showed a reduced percent change afforestation of up to a 2.8 % due to low inoculum potential limiting seedling growth. This reduction limited fuel availability and thus, cumulative area burned. Regardless of inclusion of EMF effects in simulations, landscape flammability was lower for simulations driven by the wetter and cooler CCCMA model than the warmer and drier ECHAM5 model, while tundra afforestation was greater. Results suggest abiotic factors are the primary driver of tree migration. Simulations including EMF effects, a biotic factor, yielded more conservative estimates of land cover change across Alaska that better-matched empirical estimates from the previous century. C1 [Hewitt, Rebecca E.; Bennett, Alec P.; Breen, Amy L.; Rupp, T. Scott] Univ Alaska Fairbanks, Int Arctic Res Ctr, Scenarios Network Alaska & Arctic Planning, POB 757245, Fairbanks, AK 99775 USA. [Hollingsworth, Teresa N.] Univ Alaska Fairbanks, US Forest Serv, PNW Res Stn, POB 756780, Fairbanks, AK 99775 USA. [Taylor, D. Lee] Univ New Mexico, Dept Biol, 167 Castetter Hall, Albuquerque, NM 87131 USA. [Chapin, F. Stuart, III] Univ Alaska Fairbanks, Inst Arctic Biol, POB 756000, Fairbanks, AK 99775 USA. RP Hewitt, RE (reprint author), Univ Alaska Fairbanks, Int Arctic Res Ctr, Scenarios Network Alaska & Arctic Planning, POB 757245, Fairbanks, AK 99775 USA. EM rebecca.hewitt@nau.edu OI Taylor, Donald/0000-0002-5985-9210 FU Scenarios Network for Alaska and Arctic Planning; Alaska Climate Science Center; Joint Fire Science Graduate Research Innovation Award; United States Geological Survey [G10AC00588] FX The Scenarios Network for Alaska and Arctic Planning, the Alaska Climate Science Center, and the Joint Fire Science Graduate Research Innovation Award supported this research. We thank Shalane Frost for creating Figs. 4 and 7. The project described in this publication was supported by Cooperative Agreement Number G10AC00588 from the United States Geological Survey. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the USGS. NR 68 TC 0 Z9 0 U1 12 U2 31 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAY PY 2016 VL 31 IS 4 BP 895 EP 911 DI 10.1007/s10980-015-0306-1 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DG8FZ UT WOS:000372319700015 ER PT J AU Wiberley-Bradford, AE Busse, JS Bethke, PC AF Wiberley-Bradford, Amy E. Busse, James S. Bethke, Paul C. TI Temperature-dependent regulation of sugar metabolism in wild-type and low-invertase transgenic chipping potatoes during and after cooling for low-temperature storage SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Cold-induced sweetening; Low-temperature sweetening; ADP-glucose pyrophosphorylase; Granule-bound starch synthase; beta-amylase; Solanum tuberosum ID FREE AMINO-ACIDS; SUCROSE-PHOSPHATE SYNTHASE; SOLANUM-TUBEROSUM L.; ACRYLAMIDE FORMATION; RUSSET BURBANK; COLD-STORAGE; SWEETENING RESISTANCE; MAILLARD REACTION; BETA-AMYLASE; TERM STORAGE AB Regulation of sugar metabolism in potato tubers stored at low temperatures has significant ramifications for producers and consumers of potato chips and French fries. Low-temperature storage reduces losses due to sprouting and disease but induces undesirable accumulation of the reducing sugars glucose and fructose. These sugars react with free amino acids during frying to produce dark-colored and bitter tasting products that have elevated levels of acrylamide, a suspected human carcinogen. Elevated amounts of tuber glucose and fructose are produced when vacuolar acid invertase (VInv) hydrolyses sucrose derived from starch degradation. In this study, wild-type tubers and tubers in which VInv expression was reduced by RNA interference were used to study the temperature-dependent regulation of sugar accumulation and expression of carbohydrate metabolism genes during and after the cooling of tubers to 9 or 3 degrees C. It was found that transcriptional control of genes central to carbohydrate metabolism occurred in three overlapping phases. First, early in cooling, slight increases in VInv and beta-amylase expression occurred. Second, at lower temperatures, large decreases in AGPase and GBSS expression were observed. Finally, when tubers reached 3-5 degrees C, large increases in beta-amylase expression were observed, coinciding with large increases in sucrose in all genotypes and large increases in glucose and fructose in tubers with high VInv expression. Suppression of VInv expression did not alter the expression of beta-amylase,AGPase, GBSS, or other genes, indicating that there was little or no feedback from reducing sugars to transcriptional control of carbohydrate metabolism. Invertase activity of WT tubers stored at 9 degrees C was comparable to that of WT tubers stored at 3 degrees C, but reducing sugars were markedly higher at the lower temperature. Taken together, these data support a model in which reducing sugar accumulation is controlled both by temperature-regulated changes in sucrose accumulation, resulting from increased starch degradation by beta-amylase and decreased starch resynthesis by AGPase and GBSS, and by differences in VInv activity that persist throughout storage. (C) 2015 Elsevier B.V. All rights reserved. C1 [Wiberley-Bradford, Amy E.; Bethke, Paul C.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Busse, James S.; Bethke, Paul C.] USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. RP Bethke, PC (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM paul.bethke@ars.usda.gov FU USDA NIFA Specialty Crop Research Initiative [2011-51181-30629] FX The authors thank Dr. Diming Jiang and Dr. Lei Wu for generously providing the transgenic potato lines. Funding for AEW was provided by the USDA NIFA Specialty Crop Research Initiative project "Improved breeding and variety evaluation methods to reduce acrylamide content and increase quality in processed potato products" (award number 2011-51181-30629). NR 56 TC 1 Z9 1 U1 9 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD MAY PY 2016 VL 115 BP 60 EP 71 DI 10.1016/j.postharvbio.2015.12.020 PG 12 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA DH4RH UT WOS:000372772600007 ER PT J AU Lim, J Kim, G Mo, C Kim, MS Chao, K Qin, J Fu, XP Baek, I Cho, BK AF Lim, Jongguk Kim, Giyoung Mo, Changyeun Kim, Moon S. Chao, Kuanglin Qin, Jianwei Fu, Xiaping Baek, Insuck Cho, Byoung-Kwan TI Detection of melamine in milk powders using near-infrared hyperspectral imaging combined with regression coefficient of partial least square regression model SO TALANTA LA English DT Article DE Hyperspectral imaging; Milk powder adulteration; Melamine; Partial least square regression; Regression coefficient ID INFANT FORMULA; CYANURIC ACID; LIQUID MILK; SPECTROSCOPY; PRODUCTS; CHROMATOGRAPHY; QUALITY; SAFETY AB Illegal use of nitrogen-rich melamine (C3H6N6) to boost perceived protein content of food products such as milk, infant formula, frozen yogurt, pet food, biscuits, and coffee drinks has caused serious food safety problems. Conventional methods to detect melamine in foods, such as Enzyme-linked immunosorbent assay (ELISA), High-performance liquid chromatography (HPLC), and Gas chromatography-mass spectrometry (GC-MS), are sensitive but they are time-consuming, expensive, and labor-intensive. In this research, near-infrared (NIR) hyperspectral imaging technique combined with regression coefficient of partial least squares regression (PLSR) model was used to detect melamine particles in milk powders easily and quickly. NIR hyperspectral reflectance imaging data in the spectral range of 990-1700 nm were acquired from melamine-milk powder mixture samples prepared at various concentrations ranging from 0.02% to 1%. PLSR models were developed to correlate the spectral data (independent variables) with melamine concentration (dependent variables) in melamine-milk powder mixture samples. PLSR models applying various pretreatment methods were used to reconstruct the two-dimensional PLS images. PLS images were converted to the binary images to detect the suspected melamine pixels in milk powder. As the melamine concentration was increased, the numbers of suspected melamine pixels of binary images were also increased. These results suggested that NIR hyperspectral imaging technique and the PLSR model can be regarded as an effective tool to detect melamine particles in milk powders. (C) 2016 Elsevier B.V. All rights reserved. C1 [Lim, Jongguk; Kim, Giyoung; Mo, Changyeun] Rural Dev Adm, Natl Inst Agr Sci, Jeonju 54875, South Korea. [Kim, Moon S.; Chao, Kuanglin; Qin, Jianwei] USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Fu, Xiaping] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310029, Peoples R China. [Baek, Insuck; Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Bioind Machinery Engn, Daejeon 34134, South Korea. RP Kim, MS (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM moon.kim@ars.usda.gov FU National Institute of Agricultural Science (NIAS), Rural Development Administration (RDA), Republic of Korea [PJ01008101] FX Authors would like to thank Ms. Diane Chan of EMFSL, ARS, USDA, for her technical assistance and proofreading the manuscript. This study was carried out with the support of "Research Program for Agricultural Science & Technology Development (Grant no. PJ01008101)", National Institute of Agricultural Science (NIAS), Rural Development Administration (RDA), Republic of Korea. NR 23 TC 4 Z9 4 U1 12 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0039-9140 EI 1873-3573 J9 TALANTA JI Talanta PD MAY 1 PY 2016 VL 151 BP 183 EP 191 DI 10.1016/j.talanta.2016.01.035 PG 9 WC Chemistry, Analytical SC Chemistry GA DH4NE UT WOS:000372761900024 PM 26946026 ER PT J AU Webster, TM Grey, TL Scully, BT Johnson, WC Davis, RF Brenneman, TB AF Webster, Theodore M. Grey, Timothy L. Scully, Brian T. Johnson, W. Carroll, III Davis, Richard F. Brenneman, Timothy B. TI Yield potential of spring-harvested sugar beet (Beta vulgaris) depends on autumn planting time SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Autumn sugar beet; Bioenergy crop; Biofuel crop; Energy beet; Ethanol crop; Sugar feedstock ID L. AB Sugar crops grown for biofuel production provide a source of simple sugars that can readily be made into advanced biofuels. In the mild climate of the southeastern USA, sugar beet can be grown as a winter crop, providing growers with an alternative crop that is complementary to existing summer crops. Experiments evaluated autumn planting dates from September to December on the yield of five varieties of sugar beet harvested in the spring. A linear relationship existed between sugar beet canopy width and thermal time. Plant canopy diameter increased <50% between February and May for September-planted beets, while the November-planted beets increased 180-230% during this same interval. The earlier diversion of energies to root production in September-planted beets relative to November-planted beets was reflected in yield. Sugar beet yield increased with increasing thermal time (T-base =3 degrees C) and season length. The earliest planted beets in the autumn had the highest yields, with average yields ranging from 71 to 118 t ha(-1) in the spring. The lowest harvested yields (42-69 t ha(-1)) were produced when beets were planted in late December 2012 and mid-November 2013. Yields were variable, but equivalent to summer production in Midwest USA. There were no detectable differences in planting dates or among varieties for sugar content in the sugar beets, with Brix averaging 16.1%. One of the potential limitations of autumn-planted sugar beet production in the Southeast USA is the minimum winter temperatures; beets planted in late autumn were stressed with cold temperatures in the early winter. Additional research is needed to evaluate cold tolerance on the various stages of sugar beet growth. Published by Elsevier B.V. C1 [Webster, Theodore M.; Scully, Brian T.; Johnson, W. Carroll, III; Davis, Richard F.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31794 USA. [Grey, Timothy L.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA 31794 USA. [Brenneman, Timothy B.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31794 USA. [Scully, Brian T.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Webster, TM (reprint author), USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31794 USA. EM ted.webster@ars.usda.gov RI Webster, Theodore/A-4468-2009 OI Webster, Theodore/0000-0002-8259-2059 FU University of Georgia FX Salaries, supplies, and equipment were provided by USDA-ARS and the University of Georgia. Betaseed provided in-kind supplies, equipment, and an unrestricted gift to the University of Georgia in support of research programs. NR 18 TC 0 Z9 0 U1 5 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2016 VL 83 BP 55 EP 60 DI 10.1016/j.indcrop.2015.12.037 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA DE8NX UT WOS:000370894000009 ER PT J AU Pavlista, AD Hergert, GW Margheim, JM Isbell, TA AF Pavlista, A. D. Hergert, G. W. Margheim, J. M. Isbell, T. A. TI Growth of spring camelina (Camelina sativa) under deficit irrigation in Western Nebraska SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Oilseed; Biodiesel; Omega-3; Omega-9 ID SOWING DATE; HIGH-PLAINS; JET FUEL; OIL; YIELD; BIODIESEL; NITROGEN; CANOLA; CROPS; SEED AB The High Plains of the U.S.A. is subject to periodic drought where low-water using crops are desired. Camelina is a potential biofuel crop that may be suitable for this region. The objective was to determine the growth, seed yield, and oil characteristic of camelina exposed to four levels of applied water in western Nebraska. The cultivar Cheyenne was exposed to rain-fed only (RF), and irrigated with 10 (LI), 20 (MI), and 30 (HI) cm water. Irrigation increased plant growth as measured by canopy height, stem length, and canopy weight. Maximum height (70-80 cm) was reached by 10 weeks after planting (WAP) with a total of 23 cm of applied water or 13 cm of irrigation. By 13 WAP, canopy and pod fresh weights were increased by 40-50% by the MI and HI irrigations. Likewise, at harvest (13 WAP), plant dry weight was increased by 50% by the two higher irrigation levels. Seed yields were increased by each incremental increase in irrigation from 890 kg/ha for RF to 2540 for HI, a 2.85-fold increase. Oil content was not affected by irrigating at the LI or MI but was increased only by the HI irrigation level. The fatty acid profile was altered by irrigation with an increase in the major constituent, linolenic acid, and a corresponding decrease in two other large constituents, oleic and linoleic acids. There also was a slight increase in the minor constituent of erucic acid. The growth pattern of camelina showed that 23 cm of applied water would be required for healthy plant growth but as much as 43 cm would be needed for maximum yield. The results indicate that camelina could be grown successfully in western Nebraska, supplying oil for fuel and cooking. (c) 2015 Elsevier B.V. All rights reserved. C1 [Pavlista, A. D.; Hergert, G. W.; Margheim, J. M.] Univ Nebraska, PREC, Scottsbluff, NE 69361 USA. [Isbell, T. A.] USDA ARS, Peoria, IL 61604 USA. RP Pavlista, AD (reprint author), Univ Nebraska, PREC, Scottsbluff, NE 69361 USA. EM apavlista@unl.edu FU USDA Risk Management Assessment program FX The authors are grateful for the technical support of Allison Hazen and the assistance of summer interns. I give special appreciation for the financial support of the USDA Risk Management Assessment program. NR 43 TC 6 Z9 6 U1 5 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2016 VL 83 BP 118 EP 123 DI 10.1016/j.indcrop.2015.12.017 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA DE8NX UT WOS:000370894000017 ER PT J AU Pavlista, AD Hergert, GW Margheim, JM Isbell, TA AF Pavlista, A. D. Hergert, G. W. Margheim, J. M. Isbell, T. A. TI Growth of spring canola (Brassica napus) under deficit irrigation in Western Nebraska SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Oilseed; Bio-diesel; Oleic acid; Omega-9 ID NORTHERN GREAT-PLAINS; WATER-USE EFFICIENCY; OILSEED CROPS; YIELD COMPONENTS; CROPPING SYSTEMS; SEED YIELD; QUALITY; STRESS; NITROGEN; MUSTARD AB Drought is a periodic occurrence in the High Plains of the U.S.A.; thereby low-water using crops are desired. Canola, a biofuel and food oil, has been studied in the region under rain-fed conditions for several years. The study's objective was to measure plant growth, seed yield, and oil characteristic of canola at four levels of applied water in western Nebraska. The cultivar Hyola 357 Magnum was exposed to rain-fed only (RF), and irrigated with 10 (LI), 20 (MI), and 30 (HI) cm water. Adding irrigation increased plant growth, i.e., canopy height, stem length, and canopy weight. Highest canopy height (>100 cm for MI and HI) was reached by 10 weeks after planting (WAP) with a total of 23 cm of applied water or 13 cm of irrigation. By 8 WAP, canopy fresh weight was increased by 37 to 58% by MI and HI irrigations. Pod fresh and dry weights at harvest (13 WAP) were increased >50% and >20%, respectively, by all irrigations. Seed yield were increased by each incremental increase in irrigation from 1220 kg/ha for RF to 2905 for HI, a 2.4-fold increase. Seed oil content was increased by MI and HI irrigations (4-8%). The fatty acid profile was not substantially altered by irrigation. There was a slight decrease of palmitic acid and increase in linolenic acid with HI. Canola showed that >20 cm of applied water is needed for good vine and pod growth but >40 cm was needed to achieve the highest yield. Canola could be grown successfully in western Nebraska, supplying oil for fuel and cooking. (C) 2015 Elsevier B.V. All rights reserved. C1 [Pavlista, A. D.; Hergert, G. W.; Margheim, J. M.] Univ Nebraska, PREC, Scottsbluff, NE 69361 USA. [Isbell, T. A.] USDA ARS, Peoria, IL 61604 USA. RP Pavlista, AD (reprint author), Univ Nebraska, PREC, Scottsbluff, NE 69361 USA. EM apavlista@unl.edu FU USDA Risk Management Assessment program FX The authors are grateful for the technical support of Allison Hazen and the assistance of summer interns. I give special appreciation for the financial support of the USDA Risk Management Assessment program. NR 44 TC 1 Z9 1 U1 6 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2016 VL 83 BP 635 EP 640 DI 10.1016/j.indcrop.2015.12.059 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA DE8NX UT WOS:000370894000081 ER PT J AU Houborg, R McCabe, MF Gao, F AF Houborg, Rasmus McCabe, Matthew F. Gao, Feng TI A Spatio-Temporal Enhancement Method for medium resolution LAI (STEM-LAI) SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Landsat; MODIS; STARFM; LAI; Data fusion; Downscaling; Multi-scale; Regression tree; Spatio-temporal enhancement; Data mining ID LEAF-AREA INDEX; MODIS SURFACE REFLECTANCE; LANDSAT DATA; CHLOROPHYLL CONTENT; MODEL; ALGORITHM; PRODUCTS; IMAGERY; CANOPY; FUSION AB Satellite remote sensing has been used successfully to map leaf area index (LAI) across landscapes, but advances are still needed to exploit multi-scale data streams for producing LAI at both high spatial and temporal resolution. A multi-scale Spatio-Temporal Enhancement Method for medium resolution LAI (STEM-LAI) has been developed to generate 4-day time-series of Landsat-scale LAI from existing medium resolution LAI products. STEM-LAI has been designed to meet the demands of applications requiring frequent and spatially explicit information, such as effectively resolving rapidly evolving vegetation dynamics at sub-field (30 m) scales. In this study, STEM-LAI is applied to Moderate Resolution Imaging Spectroradiometer (MODIS) based LAI data and utilizes a reference-based regression tree approach for producing MODIS-consistent, but Landsat-based, LAI. The Spatial and Temporal Adaptive Reflectance Fusion Model (STARFM) is used to interpolate the downscaled LAI between Landsat acquisition dates, providing a high spatial and temporal resolution improvement over existing LAI products. STARFM predicts high resolution LAI by blending MODIS and Landsat based information from a common acquisition date, with MODIS data from a prediction date. To demonstrate its capacity to reproduce fine-scale spatial features observed in actual Landsat LAI, the STEM-LAI approach is tested over an agricultural region in Nebraska. The implementation of a 250 m resolution LAI product, derived from MODIS 1 km data and using a scale consistent approach based on the Normalized Difference Vegetation Index (NDVI), is found to significantly improve accuracies of spatial pattern prediction, with the coefficient of efficiency (E) ranging from 0.77-0.94 compared to 0.01-0.85 when using 1 km LAI inputs alone. Comparisons against an 11-year record of in-situ measured LAI over maize and soybean highlight the utility of STEM-LAI in reproducing observed LAI dynamics (both characterized by r(2) = 0.86) over a range of plant development stages. Overall, STEM-LAI represents an effective downscaling and temporal enhancement mechanism that predicts in-situ measured LAI better than estimates derived through linear interpolation between Landsat acquisitions. This is particularly true when the in-situ measurement date is greater than 10 days from the nearest Landsat acquisition, with prediction errors reduced by up to 50%. With a streamlined and completely automated processing interface, STEM-LAI represents a flexible tool for LAI disaggregation in space and time that is adaptable to different land cover types, landscape heterogeneities, and cloud cover conditions. (C) 2015 Elsevier B.V. All rights reserved. C1 [Houborg, Rasmus; McCabe, Matthew F.] KAUST, Biol & Environm Sci & Engn Div, Water Desalinat & Reuse Ctr, Thuwal 23955, Saudi Arabia. [Gao, Feng] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. RP Houborg, R (reprint author), KAUST, Biol & Environm Sci & Engn Div, Water Desalinat & Reuse Ctr, HALO, Thuwal 23955, Saudi Arabia. EM rasmus.houborg@kaust.edu.sa FU King Abdullah University of Science and Technology (KAUST) FX The research undertaken here was funded by the King Abdullah University of Science and Technology (KAUST). We greatly appreciate the biological datasets provided by the Center for Advanced Land Management Information Technologies (CALMIT) and the Carbon Sequestration Program, University of Nebraska-Lincoln. NR 42 TC 6 Z9 6 U1 2 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD MAY PY 2016 VL 47 BP 15 EP 29 DI 10.1016/j.jag.2015.11.013 PG 15 WC Remote Sensing SC Remote Sensing GA DF1KQ UT WOS:000371099000002 ER PT J AU Atoum, Y Afridi, MJ Liu, XM McGrath, JM Hanson, LE AF Atoum, Yousef Afridi, Muhammad Jamal Liu, Xiaoming McGrath, J. Mitchell Hanson, Linda E. TI On developing and enhancing plant-level disease rating systems in real fields SO PATTERN RECOGNITION LA English DT Article DE CLS Rater; Histogram of Importance (Hol); Bag of Words (BoW); Local Binary Patterns (LBP); Superpixels ID CERCOSPORA LEAF-SPOT; SUGAR-BEET; CLASSIFICATION; SEGMENTATION; RECOGNITION; NOISE; MODEL AB Cercospora leaf spot (CLS) is one of the most serious diseases of sugar beet worldwide, and if uncontrolled, causes nearly complete defoliation and loss of revenue for beet growers. The beet sugar industry continuously seeks CLS-resistant sugar beet cultivars as one strategy to combat this disease. Normally human experts manually observe and rate the resistance of a large variety of sugar beet plants over a period of a few months. Unfortunately, this procedure is laborious and the labels vary from one expert to another resulting in disagreements on the level of resistance. Therefore, we propose a novel computer vision system, CLS Rater, to automatically and accurately rate plant images in the real field to the "USDA scale" of 0-10. Given a set of plant images captured by a tractor-mounted camera, CLS Rater extracts multi-scale superpixels, where in each scale a novel Histogram of Importances feature encodes both the within-superpixel local and across-superpixel global appearance variations. These features at different superpixel scales are then fused for learning a regressor that estimates the rating for each plant image. We further address the issue of the noisy labels by experts in the field, and propose a method to enhance the performance of the CLS Rater by automatically calibrating the experts ratings to ensure consistency. We test our system on the field data collected from two years over a two-month period for each year, under different lighting and weather conditions. Experimental results show that both the CLS Rater and the enhanced CLS Rater to be highly consistent with the rating errors of 0.65 and 0.59 respectively, which demonstrates a higher consistency than the rating standard deviation of 1.31 by human experts. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Atoum, Yousef] Michigan State Univ, Dept Elect & Comp Engn, E Lansing, MI 48824 USA. [Afridi, Muhammad Jamal; Liu, Xiaoming] Michigan State Univ, Dept Comp Sci & Engn, E Lansing, MI 48824 USA. [McGrath, J. Mitchell; Hanson, Linda E.] ARS, Sugar Beet & Bean Res Unit, USDA, Lubbock, TX USA. RP Liu, XM (reprint author), Michigan State Univ, Dept Comp Sci & Engn, E Lansing, MI 48824 USA. EM atoumyou@msu.edu; afridimu@msu.edu; liuxm@cse.msu.edu; mitchmcg@msu.edu; hansonl5@msu.edu FU Michigan Sugar Company [MSC-13-08, GR14-034] FX This project was sponsored in part by Michigan Sugar Company Competitive Grant (#MSC-13-08) and Project Greeen (#GR14-034). The authors thank Tom Goodwill and Bill Niehaus for labeling images with CLS. NR 43 TC 1 Z9 1 U1 1 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0031-3203 EI 1873-5142 J9 PATTERN RECOGN JI Pattern Recognit. PD MAY PY 2016 VL 53 BP 287 EP 299 DI 10.1016/j.patcog.2015.11.021 PG 13 WC Computer Science, Artificial Intelligence; Engineering, Electrical & Electronic SC Computer Science; Engineering GA DE8KU UT WOS:000370885700022 ER PT J AU Li, M Tshabalala, MA Buschle-Diller, G AF Li, Mei Tshabalala, Mandla A. Buschle-Diller, Gisela TI Formulation and characterization of polysaccharide beads for controlled release of plant growth regulators SO JOURNAL OF MATERIALS SCIENCE LA English DT Article ID CALCIUM ALGINATE; DELIVERY-SYSTEMS; WATER; PESTICIDE; HYDROGELS; CHITOSAN; SUPERABSORBENT; POLYACRYLAMIDE; AGROCHEMICALS; MICROSPHERES AB Owing to their chemical, physical, and functional characteristics, polysaccharides are considered to be the most versatile natural polymers. As a result, their properties have been exploited in various fields of research in the biomedical, pharmaceutical, cosmetic, food, and agricultural industries. A property of special interest is their ability to form systems or materials with unique physicochemical characteristics, such as hydrogels and micro- and nanoparticles for controlled release of active compounds. In the present study, polysaccharide beads formulated from alginate, cellulose powder, cellulose nanocrystals, starch, and xylan were reinforced with kaolin and surface-modified with polyethylenimine (PEI), a positively charged polyelectrolyte. Addition of kaolin improved the mechanical strength of the beads. Modification of the surface of the beads with PEI facilitated better control of the release rate of the plant growth regulator, phenylacetic acid (PAA). The physical properties of the beads were characterized by optical and scanning electron microscopy, and their mechanical strength was determined by an Instron 5565 Tensile Testing Machine. Cumulative release of PAA was measured by UV-Vis spectroscopy. C1 [Li, Mei; Tshabalala, Mandla A.] Auburn Univ, Dept Polymer & Fiber Engn, Auburn, AL 36849 USA. [Buschle-Diller, Gisela] US Forest Serv, USDA, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Buschle-Diller, G (reprint author), US Forest Serv, USDA, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM mzl0034@auburn.edu; mtshabalala@fs.fed.us; buschgi@auburn.edu NR 26 TC 1 Z9 1 U1 13 U2 73 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0022-2461 EI 1573-4803 J9 J MATER SCI JI J. Mater. Sci. PD MAY PY 2016 VL 51 IS 9 BP 4609 EP 4617 DI 10.1007/s10853-016-9775-0 PG 9 WC Materials Science, Multidisciplinary SC Materials Science GA DE0VB UT WOS:000370342100041 ER PT J AU Parize, DDD Foschini, MM de Oliveira, JE Klamczynski, AP Glenn, GM Marconcini, JM Mattoso, LHC AF da Silva Parize, Delne Domingos Foschini, Milene Mitsuyuki de Oliveira, Juliano Elvis Klamczynski, Artur P. Glenn, Gregory Melvin Marconcini, Jose Manoel Capparelli Mattoso, Luiz Henrique TI Solution blow spinning: parameters optimization and effects on the properties of nanofibers from poly(lactic acid)/dimethyl carbonate solutions SO JOURNAL OF MATERIALS SCIENCE LA English DT Article ID BOX-BEHNKEN DESIGN; FIBERS; FABRICATION; ACID); DIAMETER; BEHAVIOR AB Solution blow spinning (SBS) is a process to produce non-woven fiber sheets with high porosity and an extremely large amount of surface area. In this study, a Box-Behnken experimental design (BBD) was used to optimize the processing parameters for the production of nanofibers from polymer solutions consisting of poly(lactic acid) (PLA) dissolved in dimethyl carbonate. In addition, a comparative study between SBS fibers and cast film was performed to verify the influence of the SBS process on the crystallinity and thermal properties of PLA. The PLA concentration in polymer solutions was the most significant parameter affecting fiber diameter. The BBD analysis revealed that small diameter fibers were best obtained by a combination of 8 % w/v PLA concentration, 80 psi air pressure, and a feed rate of 50 A mu L min(-1). The comparative study showed that both the SBS and the film casting processes increased the PLA crystallinity. However, the PLA films had a higher degree of crystallinity compared with the fibers made by the SBS process (39 and 17 %, respectively), which was attributed to the high shear created at the SBS nozzle inducing orientation and chain alignment. During the fiber formation, crystals formed with varied morphology including the alpha'-crystals, which have a less ordered structure and lower thermal stability compared to the alpha-crystals. The lower thermal stability of SBS fibers compared to the films can be explained by the lower degree of crystallinity and also by the higher surface area which can accelerate the weight loss process. C1 [da Silva Parize, Delne Domingos; Marconcini, Jose Manoel; Capparelli Mattoso, Luiz Henrique] Fed Univ Sao Carlos UFSCar, Postgrad Program Mat Sci & Engn PPG CEM, Rodovia Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, Brazil. [da Silva Parize, Delne Domingos; Foschini, Milene Mitsuyuki; Marconcini, Jose Manoel; Capparelli Mattoso, Luiz Henrique] Embrapa Instrumentat, Natl Nanotechnol Lab Agr LNNA, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP, Brazil. [de Oliveira, Juliano Elvis] Fed Univ Lavras UFLA, Dept Engn DEG, Ave Doutor Sylvio Menicucci 1001, BR-37200000 Lavras, MG, Brazil. [da Silva Parize, Delne Domingos; Klamczynski, Artur P.; Glenn, Gregory Melvin] ARS, Bioprod Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. RP Parize, DDD (reprint author), Fed Univ Sao Carlos UFSCar, Postgrad Program Mat Sci & Engn PPG CEM, Rodovia Washington Luis,Km 235, BR-13565905 Sao Carlos, SP, Brazil.; Parize, DDD (reprint author), Embrapa Instrumentat, Natl Nanotechnol Lab Agr LNNA, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP, Brazil.; Parize, DDD (reprint author), ARS, Bioprod Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM delne.ds@gmail.com; milene.foschini@embrapa.br; juliano.materiais@gmail.com; artur.klamczynski@ars.usda.gov; greg.glenn@ars.usda.gov; jose.marconcini@embrapa.br; luiz.mattoso@embrapa.br RI Mattoso, Luiz H C/D-2794-2016 OI Mattoso, Luiz H C/0000-0001-7586-1014 FU WRRC-ARS-USDA; CAPES/CNPq FX The authors thank NatureWorks (R) for the donation of PLA samples and WRRC-ARS-USDA and CAPES/CNPq (Science without Borders Program) for the financial support. NR 28 TC 2 Z9 2 U1 6 U2 33 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0022-2461 EI 1573-4803 J9 J MATER SCI JI J. Mater. Sci. PD MAY PY 2016 VL 51 IS 9 BP 4627 EP 4638 DI 10.1007/s10853-016-9778-x PG 12 WC Materials Science, Multidisciplinary SC Materials Science GA DE0VB UT WOS:000370342100043 ER PT J AU Fleming, GM Wunderle, JM Ewert, DN AF Fleming, Genie M. Wunderle, Joseph M., Jr. Ewert, David N. TI Diet preferences of goats in a subtropical dry forest and implications for habitat management SO TROPICAL ECOLOGY LA English DT Article DE Bahamas; coppice; Erithalis fruticosa; goat grazing; Kirtland's warbler; Lantana involucrata; resource selection ID CAPRA-HIRCUS; KIRTLANDS-WARBLERS; FORAGING BEHAVIOR; BAHAMAS; COMMUNITIES; WINTER; AVAILABILITY; ISLAND; SHEEP; ECOSYSTEMS AB As part of an experimental study of using controlled goat grazing to manage winter habitat of the Kirtland's warbler (Setophaga kirtlandii), an endangered Nearctic-neotropical migratory bird, we evaluated diet preferences of domesticated goats within early successional subtropical dry forest in The Bahamas. We expected goats would show a low preference for two plants (Lantana involucrata, Erithalis fruticosa) important to the bird's winter diet and that occur in abundance in goat-grazed areas throughout the region. Contrary to our expectations, the plants were among a set of species, including Acacia choriophylla, Passiflora spp., and Thrinax morrisii, with moderate to high palatability during the mid-late dry season. Thus, strict avoidance of the two warbler food plants by goats is not a direct mechanism promoting their abundance in grazed areas. Nonetheless, grazing may still prove an economically viable means of managing existing warbler habitat by delaying succession toward a mature forest community where important food resources may be lacking. C1 [Fleming, Genie M.] Puerto Rican Conservat Fdn, POB 362495, San Juan, PR 00936 USA. [Fleming, Genie M.; Wunderle, Joseph M., Jr.] US Forest Serv, Int Inst Trop Forestry, USDA, Sabana Field Res Stn, HC 02 Box 6205, Luquillo, PR 00773 USA. [Ewert, David N.] Nature Conservancy, 101 East Grand River, Lansing, MI 48906 USA. RP Fleming, GM (reprint author), Puerto Rican Conservat Fdn, POB 362495, San Juan, PR 00936 USA.; Fleming, GM (reprint author), US Forest Serv, Int Inst Trop Forestry, USDA, Sabana Field Res Stn, HC 02 Box 6205, Luquillo, PR 00773 USA. EM fleming.gm@gmail.com RI karanki, Archana/E-7014-2016 FU International Programs of the U.S. Department of Agriculture Forest Service; Puerto Rican Conservation Foundation; Bahamas National Trust; College of the Bahamas; University of Puerto Rico; Kirtland's Warbler Recovery Team FX Funding for this study was provided by the International Programs of the U.S. Department of Agriculture Forest Service to The Nature Conservancy and the Puerto Rican Conservation Foundation working in cooperation with the Bahamas National Trust, the College of the Bahamas, the University of Puerto Rico, and the Kirtland's Warbler Recovery Team. Goats and aid with their care were provided by the Cape Eleuthera Institute and Mr. Edrin Symonette. Jennifer Howard, Ronald Lance, and Zeko McKenzie contributed to field data collection, goat management, and observation. We are especially grateful to the management and staff of Cape Eleuthera Resort, on whose grounds our research was conducted. Helpful comments on early versions of the manuscript were provided by Charles Kwit, Ariel Lugo, Joseph O'Brien, and An Peischel. NR 56 TC 0 Z9 0 U1 3 U2 25 PU INT SOC TROPICAL ECOLOGY PI VARANASI PA DEPT BOTANY BENERAS HINDU UNIV, VARANASI 221 005, INDIA SN 0564-3295 J9 TROP ECOL JI Trop. Ecol. PD MAY PY 2016 VL 57 IS 2 BP 279 EP 297 PG 19 WC Ecology SC Environmental Sciences & Ecology GA DD8XR UT WOS:000370210700015 ER PT J AU Liu, M Li, SX Swaminathan, S Sahu, BB Leandro, LF Cardinal, AJ Bhattacharyya, MK Song, QJ Walker, DR Cianzio, SR AF Liu, Min Li, Shuxian Swaminathan, Sivakumar Sahu, Binod B. Leandro, Leonor F. Cardinal, Andrea J. Bhattacharyya, Madan K. Song, Qijian Walker, David R. Cianzio, Silvia R. TI Identification of a soybean rust resistance gene in PI 567104B SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID PHAKOPSORA-PACHYRHIZI; UNITED-STATES; 1ST REPORT; GERMPLASM ACCESSIONS; CONFERS RESISTANCE; LOCUS; PARAGUAY; RPP1; CONFIRMATION; INHERITANCE AB Key message Using a combination of phenotypic screening and molecular, statistical, and linkage analyses, we have mapped a dominant soybean rust resistance gene in soybean PI 567104B. Asian soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi Syd. and P. Syd., is one of the most economically important diseases that affect soybean production worldwide. A long-term strategy for minimizing the effects of SBR is the development of genetically resistant cultivars. The objectives of the study were to identify the location of a rust-resistance (Rpp) gene(s) in plant introduction (PI) 567104B, and to determine if the gene(s) in PI 567104B was different from previously mapped Rpp loci. The progeny of the cross of 'IAR 2001 BSR' x PI 567104B was phenotyped from field assays of the F (2:3) and F (4:5) generations and from a growth chamber assay of 253 F (5:6) recombinant inbred lines (RILs). For the growth chamber, the phenotyping was conducted by inoculation with a purified 2006 fungal isolate from Mississippi. A resistance gene locus on PI 567104B was mapped to a region containing the Rpp6 locus on chromosome 18. The high level of resistance of F (1) plants from two other crosses with PI 567104B as one of the parents indicated that the gene from PI 567104B was dominant. The interval containing the gene is flanked by the simple sequence repeat (SSR) markers Satt131 and Satt394, and includes the SSR markers BARCSOYSSR_18_0331 and BARCSOYSSR_18_0380. The results also indicated that the resistance gene from PI 567104B is different from the Rpp1 to the Rpp4 genes previously identified. To determine if the gene from PI 567104B is different from the Rpp6 gene from PI 567102B, additional research will be required. C1 [Liu, Min; Swaminathan, Sivakumar; Sahu, Binod B.; Bhattacharyya, Madan K.; Cianzio, Silvia R.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Liu, Min] Shenyang Agr Univ, Dept Agron, 120 Dongling Ave, Shenyang 110866, Liaoning, Peoples R China. [Li, Shuxian] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Leandro, Leonor F.] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA. [Cardinal, Andrea J.] N Carolina State Univ, Dept Agron, Raleigh, NC 27695 USA. [Cardinal, Andrea J.] Genet Projects Lead Vegetables Seeds R&D Syngenta, 3054 Cornwallis Rd, Res Triangle Pk, NC 27709 USA. [Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Walker, David R.] Univ Illinois, USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA. [Walker, David R.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Cianzio, SR (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA.; Walker, DR (reprint author), Univ Illinois, USDA ARS, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL 61801 USA.; Walker, DR (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM david.walker@ars.usda.gov; scianzio@iastate.edu OI Sahu, Binod Bihari/0000-0002-2151-0654 FU Iowa Soybean Association (ISA); ISU [4403]; United Soybean Board (USB) [528]; USDA-ARS, Crop Genetics Research Unit, Stoneville, Mississippi [6402-21220-012-00D]; Home Economic and Agricultural Experiment Station, College of Agriculture, Iowa State University FX The study was funded by grants from the Iowa Soybean Association (ISA), ISU Project 4403 and the United Soybean Board (USB), Grant # 528, ISU Project Number 4403. The study was also partially supported by the USDA-ARS Projects 6402-21220-012-00D, Crop Genetics Research Unit, Stoneville, Mississippi, and by the Home Economic and Agricultural Experiment Station, College of Agriculture, Iowa State University. Appreciation is extended to the following collaborators of Iowa State University: Dr. Michelle Graham (ARS-USDA), and to graduate students Chantal Liepold, Alexander Luckew, Jordan Baumbach and to Assistant Researchers Peter Lundeen for their help, advice and support during the conduct of this work, to Gregory G. Gebhart for driving the plants to the USDA lab in Stoneville, Mississippi for SBR resistance screening, and to Nieves Rivera-Velez, for the scoring of the plants in the field tests conducted at Quincy FL. We also appreciate the help that David Wright and Jim Marois of the University of Florida provided in planting and managing the plots in Quincy, FL., and to Dr. Zenglu Li, Dr. Donna Harris, of the University of Georgia for providing molecular markers, and F1 hybrid seed obtained with PI 567104B. Appreciation is also extended to Drs. James Buck, Dan Phillips, and Roger Boerma of the University of Georgia for providing suggestions and reviews of the manuscript. Our gratitude also goes out to Drs. Perry Cregan and David Hyten, who were both at the USDA-ARS Soybean Genomics and Improvement Laboratory in Beltsville, MD at the time this research was conducted, and provided support in the molecular analysis of the RILs. NR 66 TC 0 Z9 0 U1 2 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2016 VL 129 IS 5 BP 863 EP 877 DI 10.1007/s00122-015-2651-5 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8QX UT WOS:000374478600001 PM 26952252 ER PT J AU Kariyawasam, GK Carter, AH Rasmussen, JB Faris, J Xu, SS Mergoum, M Liu, ZH AF Kariyawasam, Gayan K. Carter, Arron H. Rasmussen, Jack B. Faris, Justin Xu, Steven S. Mergoum, Mohamed Liu, Zhaohui TI Genetic relationships between race-nonspecific and race-specific interactions in the wheat-Pyrenophora tritici-repentis pathosystem SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID TAN SPOT RESISTANCE; QUANTITATIVE TRAIT LOCI; STAGONOSPORA NODORUM BLOTCH; HOST-SELECTIVE TOXINS; CHROMOSOMAL LOCATION; TETRAPLOID WHEAT; PTR TOXA; HEXAPLOID WHEAT; WINTER-WHEAT; LINKAGE MAP AB Key message We identified a major QTL conferring race-nonspecific resistance and revealed its relationships with race-specific interactions in the wheat- Pyrenophora tritici - repentis pathosystem. Tan spot, caused by the fungus Pyrenophora tritici-repentis (Ptr), is a destructive disease of wheat worldwide. The disease system is known to include inverse gene-for-gene, race-specific interactions involving the recognition of fungal-produced necrotrophic effectors (NEs) by corresponding host sensitivity genes. However, quantitative trait loci (QTLs) conferring race-nonspecific resistance have also been identified. In this work, we identified a major race-nonspecific resistance QTL and characterized its genetic relationships with the NE-host gene interactions Ptr ToxA-Tsn1 and Ptr ToxC-Tsc1 in a recombinant inbred wheat population derived from the cross between 'Louise' and 'Penawawa.' Both parental lines were sensitive to Ptr ToxA, but Penawawa and Louise were highly resistant and susceptible, respectively, to conidial inoculations of all races. Resistance was predominantly governed by a major race-nonspecific QTL on chromosome arm 3BL for resistance to all races. Another significant QTL was detected at the distal end of chromosome arm 1AS for resistance to the Ptr ToxC-producing isolates, which corresponded to the known location of the Tsc1 locus. The effects of the 3B and 1A QTLs were largely additive, and the 3B resistance QTL was epistatic to the Ptr ToxA-Tsn1 interaction. Resistance to race 2 in F-1 plants was completely dominant; however, race 3-inoculated F-1 plants were only moderately resistant because they developed chlorosis presumably due to the Ptr ToxC-Tsc1 interaction. This work provides further understanding of genetic resistance in the wheat-tan spot system as well as important guidance for tan spot resistance breeding. C1 [Kariyawasam, Gayan K.; Rasmussen, Jack B.; Liu, Zhaohui] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Carter, Arron H.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Faris, Justin; Xu, Steven S.] USDA ARS, Cereal Crops Res Unit, No Crop Sci Lab, Fargo, ND 58102 USA. [Mergoum, Mohamed] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Mergoum, Mohamed] Univ Georgia, Dept Crop & Soil Sci, 1109 Expt St, Griffin, GA 30223 USA. RP Liu, ZH (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. EM zhh.liu@ndsu.edu FU National Institute of Food and Agriculture, United States Department of Agriculture (USDA) [ND02224]; National Research Initiative Competitive Grants CAP from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX We thank Dr. Qijun Zhang for helping with statistical test of population homogeneity. This material is based upon work supported, in part, by the National Institute of Food and Agriculture, United States Department of Agriculture (USDA), under Hatch project number ND02224 to Z. Liu and by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture to A.H. Carter. NR 50 TC 1 Z9 1 U1 5 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2016 VL 129 IS 5 BP 897 EP 908 DI 10.1007/s00122-016-2670-x PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8QX UT WOS:000374478600003 PM 26796533 ER PT J AU Endelman, JB Jansky, SH AF Endelman, Jeffrey B. Jansky, Shelley H. TI Genetic mapping with an inbred line-derived F2 population in potato SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID CULTIVATED DIPLOID POTATOES; PIGMENTED TUBER FLESH; SOLANUM-TUBEROSUM; LINKAGE MAP; SHOOT REGENERATION; EYE DEPTH; CONSTRUCTION; INHERITANCE; SHAPE; POLYMORPHISM AB Key message This is the first report of the production and use of a diploid inbred line-based F2 population for genetic mapping in potato. Potato (Solanum tuberosum L.) is an important global food crop, for which tetrasomic inheritance and self-incompatibility have limited both genetic discovery and breeding gains. We report here on the creation of the first diploid inbred line-derived F2 population in potato, and demonstrate its utility for genetic mapping. To create the population, the doubled monoploid potato DM1-3 was crossed as a female to M6, an S-7 inbred line derived from the wild relative S. chacoense, and a single F1 plant was then self-pollinated. A genetic linkage map with 2264 single nucleotide polymorphisms was constructed and used to improve the physical anchoring of superscaffolds in the potato reference genome, which is based on DM1-3. Segregation was observed for skin and flesh color, skin and flesh pigment intensity, tuber shape, anther development, jelly end, and the presence of eye tubers instead of normal sprouts. Using the R/qtl software, we detected 10 genes, 7 of which have been previously mapped and 3 for which this is the first publication. The latter category includes tightly linked genes for the jelly end and eye tuber traits on chromosome 5. The development of recombinant inbred lines from this F2 population by single-seed descent is underway and should facilitate even better resolution of these and other loci. C1 [Endelman, Jeffrey B.; Jansky, Shelley H.] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Jansky, Shelley H.] USDA ARS, Madison, WI 53706 USA. RP Jansky, SH (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Jansky, SH (reprint author), USDA ARS, Madison, WI 53706 USA. EM shjansky@wisc.edu FU USDA NIFA [2014-67013-22434] FX The authors acknowledge the contributions made by David Douches (Michigan State University), who provided SNP data; Grace Christensen, who extracted DNA for SNP genotyping; Andy Hamernik, who was responsible for the greenhouse and field trials; and Xiaoxi Liu (Ohio State University), who scored tuber shape in 2014. This project was supported by USDA NIFA 2014-67013-22434. NR 53 TC 4 Z9 5 U1 6 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2016 VL 129 IS 5 BP 935 EP 943 DI 10.1007/s00122-016-2673-7 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8QX UT WOS:000374478600006 PM 26849236 ER PT J AU Li, S Pan, YP Wen, CL Li, YH Liu, XF Zhang, XL Behera, TK Xing, GM Weng, YQ AF Li, Sen Pan, Yupeng Wen, Changlong Li, Yuhong Liu, Xiaofeng Zhang, Xiaolan Behera, Tusar K. Xing, Guoming Weng, Yiqun TI Integrated analysis in bi-parental and natural populations reveals CsCLAVATA3 (CsCLV3) underlying carpel number variations in cucumber SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; STEM-CELL HOMEOSTASIS; SHOOT APICAL MERISTEM; RECEPTOR-LIKE KINASE; BULKED SEGREGANT ANALYSIS; FLORAL ORGAN NUMBER; MELON C. MELO; ARABIDOPSIS SHOOT; SATIVUS L.; LOCULE NUMBER AB Key message Carpel number variation in cucumber was controlled by a single gene, Cn . Linkage and association analysis revealed CsCLV3 as the candidate gene of the Cn locus. Carpel number (CN) is an important fruit quality trait of cucumber, but the genetic basis of CN variations is largely unknown. In the present study, segregating analysis in multiple bi-parental mapping populations (F-2, F-3, and RILs) derived from WI2757 (CN = 3) x True Lemon (CN = 5) suggested that CN is controlled by a simply inherited gene, Cn, with CN = 3 being incompletely dominant to CN = 5. Initial linkage mapping located Cn in a 1.9-Mb region of cucumber chromosome 1. Exploration of DNA sequence variations in this region with in silico bulked segregant analysis among eight re-sequenced lines allowed delimiting the Cn locus to a 16-kb region with five predicted genes including CsCLV3, a homolog of the Arabidopsis gene CLAVATA3. Fine genetic mapping in F-2 and RIL populations and association analysis in natural populations confirmed CsCLV3 as the candidate gene for Cn, which was further evidenced from gene expression analysis and microscopic examination of floral meristem size in the two parent lines. This study highlights the importance of integrated use of linkage and association analysis as well as next-gen high-throughput sequencing in mapping and cloning genes that are difficult in accurate genotyping. The results provide new insights into the genetic control of CN variations in cucumber, which were discussed in the context of the well-characterized CLAVATA pathway for stem cell homeostasis and regulation of meristem sizes in plants. The associations of carpel number with fruit shape, size, and weight in cucumber and melon are also discussed. C1 [Li, Sen; Xing, Guoming] Shanxi Agr Univ, Hort Coll, Taigu 030801, Peoples R China. [Li, Sen; Pan, Yupeng; Wen, Changlong; Li, Yuhong; Weng, Yiqun] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Pan, Yupeng; Li, Yuhong] Northwest A&F Univ, Hort Coll, Yangling 712100, Peoples R China. [Wen, Changlong] Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China. [Wen, Changlong] Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. [Liu, Xiaofeng; Zhang, Xiaolan] China Agr Univ, Dept Vegetable Sci, Beijing 100193, Peoples R China. [Behera, Tusar K.] Indian Agr Res Inst, Div Vegetable Sci, New Delhi 10012, India. [Weng, Yiqun] USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM weng4@wisc.edu FU Shanxi Scholarship Council of China; Fulbright Program; USDA-CRIS [3655-21000-048-00D]; U.S. Department of Agriculture (USDA)-Specialty Crop Research Initiative grant (SCRI) [2011-51181-30661] FX The authors thank Kristin Haider for technical help and language editing of the manuscript. SL's work in YW's lab was partially funded by Shanxi Scholarship Council of China. TB's was supported by the Fulbright Program. This research was supported by the USDA-CRIS project 3655-21000-048-00D and a U.S. Department of Agriculture (USDA)-Specialty Crop Research Initiative grant (SCRI, project# 2011-51181-30661) to YW. Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 85 TC 2 Z9 2 U1 8 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2016 VL 129 IS 5 BP 1007 EP 1022 DI 10.1007/s00122-016-2679-1 PG 16 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DJ8QX UT WOS:000374478600011 PM 26883041 ER PT J AU Kim, SM Dien, BS Singh, V AF Kim, Sun Min Dien, Bruce S. Singh, Vijay TI Promise of combined hydrothermal/chemical and mechanical refining for pretreatment of woody and herbaceous biomass SO BIOTECHNOLOGY FOR BIOFUELS LA English DT Review DE Combined pretreatment; Chemical pretreatment; Hydrothermal pretreatment; Mechanical refining; Lignocellulosic biofuel ID FERMENTING SACCHAROMYCES-CEREVISIAE; IMPROVE ENZYMATIC SACCHARIFICATION; DISK MILLING PRETREATMENT; HOT-COMPRESSED WATER; PALM MESOCARP FIBER; ETHANOL-PRODUCTION; CORN STOVER; LIGNOCELLULOSIC BIOMASS; BIOETHANOL PRODUCTION; CELLULOSIC ETHANOL AB Production of advanced biofuels from woody and herbaceous feedstocks is moving into commercialization. Biomass needs to be pretreated to overcome the physicochemical properties of biomass that hinder enzyme accessibility, impeding the conversion of the plant cell walls to fermentable sugars. Pretreatment also remains one of the most costly unit operations in the process and among the most critical because it is the source of chemicals that inhibit enzymes and microorganisms and largely determines enzyme loading and sugar yields. Pretreatments are categorized into hydrothermal (aqueous)/chemical, physical, and biological pretreatments, and the mechanistic details of which are briefly outlined in this review. To leverage the synergistic effects of different pretreatment methods, conducting two or more pretreatments consecutively has gained attention. Especially, combining hydrothermal/chemical pretreatment and mechanical refining, a type of physical pretreatment, has the potential to be applied to an industrial plant. Here, the effects of the combined pretreatment (combined hydrothermal/chemical pretreatment and mechanical refining) on energy consumption, physical structure, sugar yields, and enzyme dosage are summarized. C1 [Kim, Sun Min; Singh, Vijay] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. [Dien, Bruce S.] USDA ARS, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. RP Singh, V (reprint author), Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. EM vsingh@illinois.edu FU National Institute of Food and Agriculture (NIFA), NIFA (Hatch) Project "Processing Corn and Cellulosic Biomass for Food, Fuel and Industrial Products" [ILLU-741-373] FX This work was supported by a grant from National Institute of Food and Agriculture (NIFA) as a part of the NIFA (Hatch) Project No. ILLU-741-373 "Processing Corn and Cellulosic Biomass for Food, Fuel and Industrial Products." Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 75 TC 2 Z9 2 U1 11 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1754-6834 J9 BIOTECHNOL BIOFUELS JI Biotechnol. Biofuels PD APR 30 PY 2016 VL 9 AR 97 DI 10.1186/s13068-016-0505-2 PG 15 WC Biotechnology & Applied Microbiology; Energy & Fuels SC Biotechnology & Applied Microbiology; Energy & Fuels GA DK9LJ UT WOS:000375251600001 PM 27141232 ER PT J AU Harlow, BE Lawrence, LM Hayes, SH Crum, A Flythe, MD AF Harlow, Brittany E. Lawrence, Laurie M. Hayes, Susan H. Crum, Andrea Flythe, Michael D. TI Effect of Dietary Starch Source and Concentration on Equine Fecal Microbiota SO PLOS ONE LA English DT Article ID HAY-GRAIN RATIOS; STREPTOCOCCUS-BOVIS; IN-VITRO; SACCHAROMYCES-CEREVISIAE; CELLULOLYTIC BACTERIA; ENTEROCOCCUS-FAECALIS; RUMINAL BACTERIA; RUMEN BACTERIA; BAG TECHNIQUE; HORSES AB Starch from corn is less susceptible to equine small intestinal digestion than starch from oats, and starch that reaches the hindgut can be utilized by the microbiota. The objective of the current study was to examine the effects of starch source on equine fecal microbiota. Thirty horses were assigned to treatments: control (hay only), HC (high corn), HO (high oats), LC (low corn), LO (low oats), and LW (low pelleted wheat middlings). Horses received an all-forage diet (2 wk; d -14 to d -1) before the treatment diets (2 wk; d 1 to 14). Starch was introduced gradually so that horses received 50% of the assigned starch amount (high = 2 g starch/kg BW; low = 1 g starch/kg BW) by d 4 and 100% by d 11. Fecal samples were obtained at the end of the forage-only period (S0; d -2), and on d 6 (S1) and d 13 (S2) of the treatment period. Cellulolytics, lactobacilli, Group D Gram-positive cocci (GPC), lactate-utilizers and amylolytics were enumerated. Enumeration data were log transformed and analyzed by repeated measures ANOVA. There were sample day x treatment interactions (P < 0.0001) for all bacteria enumerated. Enumerations from control horses did not change during the sampling period (P > 0.05). All treatments except LO resulted in increased amylolytics and decreased cellulolytics, but the changes were larger in horses fed corn and wheat middlings (P < 0.05). Feeding oats resulted in increased lactobacilli and decreased GPC (P < 0.05), while corn had the opposite effects. LW had increased lactobacilli and GPC (P < 0.05). The predominant amylolytic isolates from HC, LC and LW on S2 were identified by 16S RNA gene sequencing as Enterococcus faecalis, but other species were found in oat fed horses. These results demonstrate that starch source can have a differential effect on the equine fecal microbiota. C1 [Harlow, Brittany E.; Lawrence, Laurie M.; Hayes, Susan H.; Crum, Andrea; Flythe, Michael D.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Flythe, Michael D.] ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY 40546 USA. RP Flythe, MD (reprint author), Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA.; Flythe, MD (reprint author), ARS, Forage Anim Prod Res Unit, USDA, Lexington, KY 40546 USA. EM michael.flythe@ars.usda.gov RI Flythe, Michael/F-2500-2010 OI Flythe, Michael/0000-0002-8868-9169 FU Prairie Oat Growers Association; USDA-ARS, National Program [215]; National Institutes of Food and Agriculture (Hatch); Prairie Oat Growers FX This work is supported by: a competitive grant from the Prairie Oat Growers Association. Principle investigator: LML, Co-investigator: MDF. The National Institutes of Food and Agriculture via Hatch: Principle investigator: LML. Salary for MDF was provided by USDA-ARS, National Program 215. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The information reported in this article (No. 15-07-084) is part of a project of the Kentucky Agricultural Experiment Station and is published with the approval of the Director. This work is supported by the National Institutes of Food and Agriculture (Hatch) and the Prairie Oat Growers. MDF was supported by USDA-ARS, National Program 215. The authors acknowledge the technical assistance of Gloria Gellin, and animal husbandry assistance of Laura Strasinger, Ashley Fowler and Tayler Hansen. NR 65 TC 0 Z9 0 U1 5 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 29 PY 2016 VL 11 IS 4 AR e0154037 DI 10.1371/journal.pone.0154037 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK8XM UT WOS:000375212600018 PM 27128793 ER PT J AU Damaska, A Konstantinov, A AF Damaska, Albert Konstantinov, Alexander TI A new species of Cangshanaltica Konstantinov et al., a moss-inhabiting flea beetle from Thailand (Coleoptera: Chrysomelidae: Galerucinae: Alticini) SO ZOOTAXA LA English DT Editorial Material C1 [Damaska, Albert] Charles Univ Prague, Fac Sci, Dept Zool, Vinicna 7, Prague 12844 2, Czech Republic. [Konstantinov, Alexander] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst,Natl Museum Nat Hist, MRC 168, Washington, DC USA. RP Damaska, A (reprint author), Charles Univ Prague, Fac Sci, Dept Zool, Vinicna 7, Prague 12844 2, Czech Republic.; Konstantinov, A (reprint author), ARS, Systemat Entomol Lab, USDA, Smithsonian Inst,Natl Museum Nat Hist, MRC 168, Washington, DC USA. EM albert.damaska@natur.cuni.cz; alex.konstantinov@ars.usda.gov NR 1 TC 0 Z9 0 U1 4 U2 5 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD APR 29 PY 2016 VL 4107 IS 1 BP 93 EP 97 PG 5 WC Zoology SC Zoology GA DK9TX UT WOS:000375275600007 PM 27394809 ER PT J AU Kelly, CN McGuire, KJ Miniat, CF Vose, JM AF Kelly, Charlene N. McGuire, Kevin J. Miniat, Chelcy Ford Vose, James M. TI Streamflow response to increasing precipitation extremes altered by forest management SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article ID EASTERN-UNITED-STATES; EMERALD ASH BORER; CLIMATE-CHANGE; WATER YIELD; ECOSYSTEM PROCESSES; MODEL PERFORMANCE; VEGETATION; HYDROLOGY; IMPACTS; PINE AB Increases in extreme precipitation events of floods and droughts are expected to occur worldwide. The increase in extreme events will result in changes in streamflow that are expected to affect water availability for human consumption and aquatic ecosystem function. We present an analysis that may greatly improve current streamflow models by quantifying the impact of the interaction between forest management and precipitation. We use daily long-term data from paired watersheds that have undergone forest harvest or species conversion. We find that interactive effects of climate change, represented by changes in observed precipitation trends, and forest management regime, significantly alter expected streamflow most often during extreme events, ranging from a decrease of 59% to an increase of 40% in streamflow, depending upon management. Our results suggest that vegetation might be managed to compensate for hydrologic responses due to climate change to help mitigate effects of extreme changes in precipitation. C1 [Kelly, Charlene N.] W Virginia Univ, Davis Coll, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [McGuire, Kevin J.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [McGuire, Kevin J.] Virginia Polytech Inst & State Univ, Virginia Water Resources Res Ctr, Blacksburg, VA 24061 USA. [Miniat, Chelcy Ford] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC USA. [Vose, James M.] N Carolina State Univ, USDA, Forest Serv, Ctr Integrated Forest Sci,Dept Forestry & Environ, Raleigh, NC 27695 USA. RP Kelly, CN (reprint author), W Virginia Univ, Davis Coll, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. EM ckelly1@mix.wvu.edu RI McGuire, Kevin/E-7770-2010 OI McGuire, Kevin/0000-0001-5751-3956 FU USDA United States Forest Service [SRS 10-CA-11330140-033] FX This study was funded by the USDA United States Forest Service (Agreement SRS 10-CA-11330140-033). Data for these analyses are maintained on the U.S. Forest Service Coweeta Hydrologic Laboratory Long-Term Ecological Research (LTER) server. Data can be accessed by a request sent to the USDA Forest Service Southern Research Station at Coweeta. NR 53 TC 2 Z9 2 U1 8 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD APR 28 PY 2016 VL 43 IS 8 BP 3727 EP 3736 DI 10.1002/2016GL068058 PG 10 WC Geosciences, Multidisciplinary SC Geology GA DP2RQ UT WOS:000378338800015 ER PT J AU Perera, OP Walsh, TK Luttrell, RG AF Perera, Omaththage P. Walsh, Thomas K. Luttrell, Randall G. TI Complete Mitochondrial Genome of Helicoverpa zea (Lepidoptera: Noctuidae) and Expression Profiles of Mitochondrial-Encoded Genes in Early and Late Embryos SO JOURNAL OF INSECT SCIENCE LA English DT Article DE bollworm; mtDNA; transcript processing ID RNA PUNCTUATION MODEL; ARMIGERA LEPIDOPTERA; DIURAPHIS-NOXIA; HELIOTHIS-ZEA; DNA ANALYSIS; TRNASCAN-SE; TRANSCRIPTION; POPULATIONS; SELECTION; NORTH AB The mitochondrial genome (mitogenome) of the bollworm, Helicoverpa zea (Boddie), was assembled using paired-end nucleotide sequence reads generated with a next-generation sequencing platform. Assembly resulted in a mitogenome of 15,348 bp with greater than 17,000-fold average coverage. Organization of the H. zea mitogenome (gene order and orientation) was identical to other known lepidopteran mitogenome sequences. Compared with Helicoverpa armigera (Hubner) mitogenome, there were a few differences in the lengths of gaps between genes, but the lengths of nucleotide overlaps were essentially conserved between the two species. Nucleotide composition of the H. zea mitochondrial genome was very similar to those of the related species H. armigera and Helicoverpa punctigera Wallengren. Mapping of RNA-Seq reads obtained from 2-h eggs and 48-h embryos to protein coding genes (PCG) revealed that all H. zea PCGs were processed as single mature gene transcripts except for the bicistronic atp8+atp6 transcript. A tRNA-like sequence predicted to form a hammer-head-like secondary structure that may play a role in transcription start and mitogenome replication was identified within the control region of the H. zea mitogenome. Similar structures were also found within the control regions of several other lepidopteran species. Expression analysis revealed significant differences in levels of expression of PCGs within each developmental stage, but the pattern of variation was similar in both developmental stages analyzed in this study. Mapping of RNA-Seq reads to PCG transcripts also identified transcription termination and polyadenylation sites that differed from the sites described in other lepidopteran species. C1 [Perera, Omaththage P.; Luttrell, Randall G.] ARS, Southern Insect Management Res Unit, USDA, Stoneville, MS 38776 USA. [Walsh, Thomas K.] Commonwealth Sci & Ind Res Org, Land & Water Flagship, Clunies Ross St,GPO Box 1700, Canberra, ACT 2601, Australia. RP Perera, OP (reprint author), ARS, Southern Insect Management Res Unit, USDA, Stoneville, MS 38776 USA. EM op.perera@ars.usda.gov; tom.walsh@csiro.au; randy.luttrell@ars.usda.gov RI Walsh, Thomas/C-1263-2009 OI Walsh, Thomas/0000-0001-8425-0135 NR 66 TC 0 Z9 0 U1 2 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD APR 28 PY 2016 VL 16 AR 40 DI 10.1093/jisesa/iew023 PG 10 WC Entomology SC Entomology GA DK8TC UT WOS:000375200200003 ER PT J AU Kuczynski, L Rector, BG Kiedrowicz, A Lewandowski, M Szydlo, W Skoracka, A AF Kuczynski, Lechoslaw Rector, Brian G. Kiedrowicz, Agnieszka Lewandowski, Mariusz Szydlo, Wiktoria Skoracka, Anna TI Thermal Niches of Two Invasive Genotypes of the Wheat Curl Mite Aceria tosichella: Congruence between Physiological and Geographical Distribution Data SO PLOS ONE LA English DT Article ID STREAK-MOSAIC-VIRUS; RECENT CLIMATE-CHANGE; DIFFERENTIAL TRANSMISSION; ACARI ERIOPHYOIDEA; INFECTING WHEAT; BEMISIA-TABACI; 1ST REPORT; TEMPERATURE; ERIOPHYIDAE; MODELS AB The wheat curl mite (WCM), Aceria tosichella Keifer, is a major pest of cereals worldwide. It is also a complex of well-defined genetic lineages with divergent physiological traits, which has not been accounted for in applied contexts. The aims of the study were to model the thermal niches of the two most pestiferous WCM lineages, designated MT-1 and MT-8, and to assess the extent to which temperature determines the distribution of these lineages. WCM population dynamics were modeled based on thermal niche data from March to November on the area of Poland (>311,000 km(2)). The most suitable regions for population development were predicted and compared to empirical field abundance data. Congruence between modeled parameters and field data for mite presence were observed for both WCM lineages although congruence between modeled thermal suitability and mite field abundance was observed only for MT-8. Thermal niche data for MT-1 and MT-8 provide biological insights and aid monitoring and management of WCM and the plant viruses it vectors. The presented models accurately estimate distributions of WCM and can be incorporated into management strategies for both current and predicted climate scenarios. C1 [Kuczynski, Lechoslaw] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Avian Biol & Ecol, Poznan, Poland. [Rector, Brian G.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV USA. [Kiedrowicz, Agnieszka; Szydlo, Wiktoria; Skoracka, Anna] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, Poznan, Poland. [Lewandowski, Mariusz] Warsaw Univ Life Sci SGGW, Fac Hort Biotechnol & Landscape Architecture, Dept Appl Entomol, Warsaw, Poland. RP Skoracka, A (reprint author), Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, Poznan, Poland. EM skoracka@amu.edu.pl RI Kuczynski, Lechoslaw/E-5525-2011; Lewandowski, Mariusz/H-2184-2011; Kiedrowicz, Agnieszka/K-5181-2016; Szydlo, Wiktoria/L-4804-2016 OI Kuczynski, Lechoslaw/0000-0003-3498-5445; Lewandowski, Mariusz/0000-0001-5360-0382; Kiedrowicz, Agnieszka/0000-0002-2425-7885; Szydlo, Wiktoria/0000-0001-5990-5611 FU National Science Centre in Poland [2011/03/B/NZ8/00129] FX The study was funded by the National Science Centre in Poland, grant no. 2011/03/B/NZ8/00129. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 1 Z9 1 U1 3 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 28 PY 2016 VL 11 IS 4 AR e0154600 DI 10.1371/journal.pone.0154600 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK8XE UT WOS:000375211700112 PM 27123590 ER PT J AU Becker, SC Roach, DR Chauhan, VS Shen, Y Foster-Frey, J Powell, AM Bauchan, G Lease, RA Mohammadi, H Harty, WJ Simmons, C Schmelcher, M Camp, M Dong, SL Baker, JR Sheen, TR Doran, KS Pritchard, DG Almeida, RA Nelson, DC Marriott, I Lee, JC Donovan, DM AF Becker, Stephen C. Roach, Dwayne R. Chauhan, Vinita S. Shen, Yang Foster-Frey, Juli Powell, Anne M. Bauchan, Gary Lease, Richard A. Mohammadi, Homan Harty, William J. Simmons, Chad Schmelcher, Mathias Camp, Mary Dong, Shengli Baker, John R. Sheen, Tamsin R. Doran, Kelly S. Pritchard, David G. Almeida, Raul A. Nelson, Daniel C. Marriott, Ian Lee, Jean C. Donovan, David M. TI Triple-acting Lytic Enzyme Treatment of Drug-Resistant and Intracellular Staphylococcus aureus SO SCIENTIFIC REPORTS LA English DT Article ID CELL-PENETRATING PEPTIDE; WALL BINDING DOMAINS; BACTERIOPHAGE ENDOLYSINS; STREPTOCOCCUS-PNEUMONIAE; ANTIBIOTIC-RESISTANCE; MAMMARY-GLANDS; NUCLEIC-ACIDS; UNITED-STATES; MURINE MODEL; IN-VIVO AB Multi-drug resistant bacteria are a persistent problem in modern health care, food safety and animal health. There is a need for new antimicrobials to replace over used conventional antibiotics. Here we describe engineered triple-acting staphylolytic peptidoglycan hydrolases wherein three unique antimicrobial activities from two parental proteins are combined into a single fusion protein. This effectively reduces the incidence of resistant strain development. The fusion protein reduced colonization by Staphylococcus aureus in a rat nasal colonization model, surpassing the efficacy of either parental protein. Modification of a triple-acting lytic construct with a protein transduction domain significantly enhanced both biofilm eradication and the ability to kill intracellular S. aureus as demonstrated in cultured mammary epithelial cells and in a mouse model of staphylococcal mastitis. Interestingly, the protein transduction domain was not necessary for reducing the intracellular pathogens in cultured osteoblasts or in two mouse models of osteomyelitis, highlighting the vagaries of exactly how protein transduction domains facilitate protein uptake. Bacterial cell wall degrading enzyme antimicrobials can be engineered to enhance their value as potent therapeutics. C1 [Becker, Stephen C.; Roach, Dwayne R.; Foster-Frey, Juli; Powell, Anne M.; Bauchan, Gary; Lease, Richard A.; Mohammadi, Homan; Harty, William J.; Simmons, Chad; Schmelcher, Mathias; Camp, Mary; Donovan, David M.] ARS, USDA, 10300 Baltimore Ave, Beltsville, MD USA. [Chauhan, Vinita S.; Marriott, Ian] Univ N Carolina, Biol, Charlotte, NC 28223 USA. [Shen, Yang; Nelson, Daniel C.] Univ MD, Inst Biosci & Biotechnol Res, Rockville, MD USA. [Shen, Yang; Nelson, Daniel C.] Univ MD, Dept Vet Med, College Pk, MD USA. [Lee, Jean C.] Brigham & Womens Hosp, Dept Med, Channing Lab, Boston, MA USA. [Lee, Jean C.] Harvard Univ, Sch Med, Boston, MA USA. [Dong, Shengli; Baker, John R.; Pritchard, David G.] Univ Alabama Birmingham, Biochem, Birmingham, AL USA. [Sheen, Tamsin R.; Doran, Kelly S.] San Diego State Univ, Biol, San Diego, CA 92182 USA. [Almeida, Raul A.] Univ Tennessee, Knoxville, TN USA. [Lease, Richard A.] Ohio State Univ, Dept Chem & Biomol Engn, Columbus, OH 43210 USA. [Shen, Yang; Schmelcher, Mathias] ETH, Inst Food Nutr & Hlth, Zurich, Switzerland. [Dong, Shengli] Louisiana State Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, New Orleans, LA USA. [Roach, Dwayne R.] Inst Pasteur, Dept Microbiol, Unite Biol Mol Gene Chez Extremophiles, Paris, France. RP Donovan, DM (reprint author), ARS, USDA, 10300 Baltimore Ave, Beltsville, MD USA. EM david.donovan@ars.usda.gov FU NIH [1RO1AI075077-01A1]; NRI [2007-35204-18395]; US State Department funds; NIAID, NIH [HHSN272200700055C] FX This work was supported in part by NIH grant 1RO1AI075077-01A1; NRI grant 2007-35204-18395 and US State Department funds supporting US-Pakistani and US-Russian collaborations. Numerous isolates were obtained through the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) program: supported under NIAID, NIH Contract No. HHSN272200700055C. Mentioning of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. We would like to thank Kelly Shields, and Meghan Dowd for excellent technical assistance. NR 57 TC 1 Z9 1 U1 7 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD APR 28 PY 2016 VL 6 AR 25063 DI 10.1038/srep25063 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK5LD UT WOS:000374960500001 PM 27121552 ER PT J AU Trigo, C Spokas, KA Hall, KE Cox, L Koskinen, WC AF Trigo, Carmen Spokas, Kurt A. Hall, Kathleen E. Cox, Lucia Koskinen, William C. TI Metolachlor Sorption and Degradation in Soil Amended with Fresh and Aged Biochars SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE availability; degradation; aging. sorption; biochar; metolachlor ID DIFFERENT PYROLYTIC TEMPERATURES; BLACK CARBON; ORGANIC-CHEMICALS; INCUBATION-TIME; S-METOLACHLOR; BIOAVAILABILITY; ATRAZINE; DESORPTION; MINERALIZATION; RESIDUES AB Addition of organic amendments such as biochar to soils can influence pesticide sorption-desorption processes and, in turn, the amount of pesticide readily availability for transport and biodegradation. Sorption-desorption processes are affected by both the physical and chemical properties of soils and pesticides, as well as soil pesticide contact time, or aging. Changes in sorption-desorption of metolachlor with aging in soil amended with three macadamia nut shell biochars aged 0 (BCmac-fr), 1 year (BCmac-1yr), and 2 years (BCmac-2yr) and two wood biochars aged 0 (BCwood-fr) and 5 years (BCwood-5yr) were determined. Apparent sorption coefficient (Kd-app) values increased with incubation time to a greater extent in amended soil as compared to unamended soils; Kd-app increased by 1.2-fold for the unamended soil, 2.0-fold for BCwood-fr, 1.4-fold for BCwood-5yr, 2.4-fold for BCmac-fr, 2.5-fold for BCmac-1yr, and 1.9-fold for BCmac-4yr. The increase in calculated Kd-app value was the result of a 15% decrease in the metolachlor solution concentration extractable with CaCl2 solution with incubation time in soil as compared to a 50% decrease in amended soil with very little change in the sorbed concentration. Differences could possibly be due to diffusion to less accessible or stronger binding sites with time, a faster rate of degradation (in solution and on labile sites) than desorption, or a combination of the two in the amended soils. These data show that transport models would overpredict the depth of movement of metolachlor in soil if effects of aging or biochar amendments are not considered. C1 [Trigo, Carmen; Hall, Kathleen E.; Koskinen, William C.] Univ Minnesota, Dept Soil Water & Climate, 1991 Upper Buford Circle, St Paul, MN 55108 USA. [Spokas, Kurt A.] USDA ARS, Room 439,1991 Upper Buford Circle, St Paul, MN 55108 USA. [Cox, Lucia] CSIC, Inst Recursos Nat & Agrobiol Sevilla IRNASEC, E-41080 Seville, Spain. RP Trigo, C (reprint author), Univ Minnesota, Dept Soil Water & Climate, 1991 Upper Buford Circle, St Paul, MN 55108 USA. EM carmentrigo1@gmail.com RI Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 FU MINECO/FECYT; [AGL2013-48446-C3-1-R] FX We acknowledge the postdoctoral fellowship MINECO/FECYT for C.T. and the funding project AGL2013-48446-C3-1-R. C.T. thanks MINECO/FECYT for a postdoctoral fellowship. NR 57 TC 0 Z9 0 U1 15 U2 43 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 27 PY 2016 VL 64 IS 16 BP 3141 EP 3149 DI 10.1021/acs.jafc.6b00246 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DK9IO UT WOS:000375244300005 PM 27050383 ER PT J AU Roseland, JM Patterson, KY Andrews, KW Phillips, KM Phillips, MM Pehrsson, PR Dufresne, GL Jakobsen, J Gusev, PA Savarala, S Nguyen, QV Makowski, AJ Scheuerell, CR Larouche, GP Wise, SA Harnly, JM Williams, JR Betz, JM Taylor, CL AF Roseland, Janet Maxwell Patterson, Kristine Y. Andrews, Karen W. Phillips, Katherine M. Phillips, Melissa M. Pehrsson, Pamela R. Dufresne, Guy L. Jakobsen, Jette Gusev, Pavel A. Savarala, Sushma Nguyen, Quynhanh V. Makowski, Andrew J. Scheuerell, Chad R. Larouche, Guillaume P. Wise, Stephen A. Harnly, James M. Williams, Juhi R. Betz, Joseph M. Taylor, Christine L. TI Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography-Mass Spectrometry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE reference material; food; dietary supplement; vitamin D-3 (cholecalciferol); 25-hydroxyvitamin D-3 (25-hydroxycholcalciferol) ID NUTRIENT ANALYSIS PROGRAM; CARDIOVASCULAR-DISEASE; BIOLOGICAL-ACTIVITY; NATIONAL FOOD; D-3; SERUM; EGGS; MEAT; HEALTH; FISH AB Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed. The primary goal of this study was to evaluate whether vitamin D-3 and 25(OH)D-3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D-3 in the DS. Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D-3 and 25(OH)D-3, in foods and a DS. C1 [Roseland, Janet Maxwell; Patterson, Kristine Y.; Andrews, Karen W.; Pehrsson, Pamela R.; Gusev, Pavel A.; Savarala, Sushma; Nguyen, Quynhanh V.; Williams, Juhi R.] USDA ARS, Nutrient Data Lab, BARC West, 10300 Baltimore Ave,Bldg 005, Beltsville, MD 20705 USA. [Phillips, Katherine M.] Virginia Tech, Biochem Dept 0308, 304 Engel Hall, Blacksburg, VA 24061 USA. [Phillips, Melissa M.; Wise, Stephen A.] NIST, Div Chem Sci, MS 8392,100 Bur Dr, Gaithersburg, MD 20899 USA. [Dufresne, Guy L.] Hlth Canada, Food & Nutr Lab, 1001 St Laurent Ouest, Longueuil, PQ J4K 1C7, Canada. [Jakobsen, Jette] Tech Univ Denmark, Natl Food Inst, Div Food Chem, Soborg, Denmark. [Makowski, Andrew J.] Heartland Assays LLC, Suite 4400,2711 South Loop Dr, Ames, IA 50010 USA. [Scheuerell, Chad R.] Covance Labs, 3301 Kinsman Blvd, Madison, WI 53704 USA. [Betz, Joseph M.; Taylor, Christine L.] NIH, Off Dietary Supplements, 3B01,MSC 7517,6100 Execut Blvd, Bethesda, MD 20892 USA. [Harnly, James M.] USDA ARS, Food Composit & Method Dev Lab, BARC East, Bldg 161,10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Roseland, JM (reprint author), USDA ARS, Nutrient Data Lab, BARC West, 10300 Baltimore Ave,Bldg 005, Beltsville, MD 20705 USA. EM janet.roseland@ars.usda.gov FU Office of Dietary Supplements of the National Institutes of Health [60 1235 3012] FX Partial funding for this work was provided under Agreement 60 1235 3012 from Office of Dietary Supplements of the National Institutes of Health. NR 47 TC 0 Z9 0 U1 4 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 27 PY 2016 VL 64 IS 16 BP 3167 EP 3175 DI 10.1021/acs.jafc.5b05016 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DK9IO UT WOS:000375244300008 PM 27045951 ER PT J AU Mama, SK Li, YS Basen-Engquist, K Lee, RE Thompson, D Wetter, DW Nguyen, NT Reitzel, LR McNeill, LH AF Mama, Scherezade K. Li, Yisheng Basen-Engquist, Karen Lee, Rebecca E. Thompson, Deborah Wetter, David W. Nguyen, Nga T. Reitzel, Lorraine R. McNeill, Lorna H. TI Psychosocial Mechanisms Linking the Social Environment to Mental Health in African Americans SO PLOS ONE LA English DT Article ID SELF-RATED HEALTH; PERCEIVED RACIAL-DISCRIMINATION; QUALITY-OF-LIFE; DEPRESSIVE SYMPTOMS; PHYSICAL-ACTIVITY; SHORT-FORM; SOCIOECONOMIC POSITION; HOST-RESISTANCE; RISK-FACTORS; SUPPORT AB Resource-poor social environments predict poor health, but the mechanisms and processes linking the social environment to psychological health and well-being remain unclear. This study explored psychosocial mediators of the association between the social environment and mental health in African American adults. African American men and women (n = 1467) completed questionnaires on the social environment, psychosocial factors (stress, depressive symptoms, and racial discrimination), and mental health. Multiplemediator models were used to assess direct and indirect effects of the social environment on mental health. Low social status in the community (p < .001) and U.S. (p < .001) and low social support (p < .001) were associated with poor mental health. Psychosocial factors significantly jointly mediated the relationship between the social environment and mental health in multiple-mediator models. Low social status and social support were associated with greater perceived stress, depressive symptoms, and perceived racial discrimination, which were associated with poor mental health. Results suggest the relationship between the social environment and mental health is mediated by psychosocial factors and revealed potential mechanisms through which social status and social support influence the mental health of African American men and women. Findings from this study provide insight into the differential effects of stress, depression and discrimination on mental health. Ecological approaches that aim to improve the social environment and psychosocial mediators may enhance health-related quality of life and reduce health disparities in African Americans. C1 [Mama, Scherezade K.] Penn State Univ, Coll Hlth & Human Dev, Dept Kinesiol, University Pk, PA 16802 USA. [Li, Yisheng; Nguyen, Nga T.] Univ Texas MD Anderson Canc Ctr, Dept Biostat, Houston, TX 77030 USA. [Basen-Engquist, Karen] Univ Texas MD Anderson Canc Ctr, Dept Behav Sci, Houston, TX 77030 USA. [Lee, Rebecca E.] Arizona State Univ, Coll Nursing & Hlth Innovat, Phoenix, AZ USA. [Thompson, Deborah] Baylor Coll Med, Childrens Nutr Res Ctr, USDA, ARS, Houston, TX 77030 USA. [Wetter, David W.] Rice Univ, Dept Psychol, Houston, TX 77251 USA. [Reitzel, Lorraine R.] Univ Houston, Coll Educ, Dept Psychol Hlth & Learning Sci, Houston, TX USA. [McNeill, Lorna H.] Univ Texas MD Anderson Canc Ctr, Dept Hlth Dispar Res, Houston, TX 77030 USA. RP Mama, SK (reprint author), Penn State Univ, Coll Hlth & Human Dev, Dept Kinesiol, University Pk, PA 16802 USA. EM skmama@psu.edu OI Mama, Scherezade/0000-0003-1935-7707; Wetter, David/0000-0002-6366-0108 FU University Cancer Foundation; Duncan Family Institute through the Center for Community-Engaged Translational Research; Ms. Regina J. Rogers Gift: Health Disparities Research Program; Cullen Trust for Health Care Endowed Chair Funds for Health Disparities Research; Morgan Foundation Funds for Health Disparities Research and Educational Programs; Center for Energy Balance in Cancer Prevention and Survivorship, Duncan Family Institute for Cancer Prevention and Risk Assessment; MD Anderson Cancer Center's Cancer Prevention Research Training Program; National Cancer Institute [R25T CA057730, P30 CA016672] FX Project CHURCH was generously supported by funding from the University Cancer Foundation; the Duncan Family Institute through the Center for Community-Engaged Translational Research; the Ms. Regina J. Rogers Gift: Health Disparities Research Program; the Cullen Trust for Health Care Endowed Chair Funds for Health Disparities Research; and the Morgan Foundation Funds for Health Disparities Research and Educational Programs. Support was also provided by the Center for Energy Balance in Cancer Prevention and Survivorship, Duncan Family Institute for Cancer Prevention and Risk Assessment. Scherezade K. Mama was supported in part by a cancer prevention fellowship through MD Anderson Cancer Center's Cancer Prevention Research Training Program, funded by the National Cancer Institute (R25T CA057730 and P30 CA016672). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 0 Z9 0 U1 3 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 27 PY 2016 VL 11 IS 4 AR e0154035 DI 10.1371/journal.pone.0154035 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK5QZ UT WOS:000374976200042 PM 27119366 ER PT J AU Yan, L Sundaram, S AF Yan, Lin Sundaram, Sneha TI Monocyte chemotactic protein-1 deficiency reduces spontaneous metastasis of Lewis lung carcinoma in mice fed a high-fat diet SO ONCOTARGET LA English DT Article DE MCP-1; pro-inflammatory cytokine; metastasis; high-fat diet; mice ID ENDOTHELIAL GROWTH-FACTOR; TUMOR-NECROSIS-FACTOR; BREAST-CANCER; CHEMOATTRACTANT PROTEIN-1; ADIPOSE-TISSUE; PROSTATE-CANCER; RADICAL PROSTATECTOMY; OBESITY; CELLS; MCP-1 AB Adipose-produced pro-inflammatory cytokines contribute to obesity and cancer. This 2x2 experiment was designed to investigate effects of monocyte chemotactic protein-1 (MCP-1) deficiency on pulmonary metastasis of Lewis lung carcinoma (LLC) in MCP-1 deficient and wild-type mice fed a modified AIN93G diet containing 16% and 45% of energy from corn oil, respectively. The high-fat diet significantly increased the number and size (cross-sectional area and volume) of lung metastases compared to the AIN93G control diet. Deficiency in MCP-1 reduced lung metastases by 37% in high-fat diet-fed mice; it reduced metastatic cross-sectional area by 46% and volume by 69% compared to wild-type mice. Adipose and plasma concentrations of MCP-1 were significantly higher in high-fat diet-fed wild-type mice than in their AIN93G-fed counterparts; they were not detectable in MCP-1 deficient mice regardless of diet. Plasma concentrations of plasminogen activator inhibitor-1, tumor necrosis factor-alpha, vascular endothelial growth factor and tissue inhibitor of metalloproteinase-1 were significantly higher in MCP-1 deficient mice compared to wild-type mice. We conclude that adipose-produced MCP-1 contributes to high-fat diet-enhanced metastasis. While MCP-1 deficiency reduces metastasis, the elevation of pro-inflammatory cytokines and angiogenic factors in the absence of MCP-1 may support the metastatic development and growth of LLC in MCP-1 deficient mice. C1 [Yan, Lin; Sundaram, Sneha] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Yan, L (reprint author), ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. EM lin.yan@ars.usda.gov FU U.S. Department of Agriculture, Agriculture Research Service [3062-51000-050-00D] FX This work was supported by the U.S. Department of Agriculture, Agriculture Research Service, Research Project 3062-51000-050-00D. NR 43 TC 2 Z9 2 U1 0 U2 0 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD APR 26 PY 2016 VL 7 IS 17 BP 24792 EP 24799 DI 10.18632/oncotarget.8364 PG 8 WC Oncology; Cell Biology SC Oncology; Cell Biology GA DO3UB UT WOS:000377706200140 PM 27028862 ER PT J AU Soy, J Leivar, P Gonzalez-Schain, N Martin, G Diaz, C Sentandreu, M Al-Sady, B Quail, PH Monte, E AF Soy, Judit Leivar, Pablo Gonzalez-Schain, Nahuel Martin, Guiomar Diaz, Celine Sentandreu, Maria Al-Sady, Bassem Quail, Peter H. Monte, Elena TI Molecular convergence of clock and photosensory pathways through PIF3-TOC1 interaction and co-occupancy of target promoters SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE PIFs; photoperiod; TOC1; circadian clock; gating of growth ID ARABIDOPSIS CIRCADIAN CLOCK; PLANT-GROWTH; LIGHT; NETWORK; PIF3; TRANSCRIPTION; RESPONSES; THALIANA; COINCIDENCE; OSCILLATOR AB A mechanism for integrating light perception and the endogenous circadian clock is central to a plant's capacity to coordinate its growth and development with the prevailing daily light/dark cycles. Under short-day (SD) photocycles, hypocotyl elongation is maximal at dawn, being promoted by the collective activity of a quartet of transcription factors, called PIF1, PIF3, PIF4, and PIF5 (phytochrome-interacting factors). PIF protein abundance in SDs oscillates as a balance between synthesis and photoactivated-phytochrome-imposed degradation, with maximum levels accumulating at the end of the long night. Previous evidence shows that elongation under diurnal conditions (as well as in shade) is also subjected to circadian gating. However, the mechanism underlying these phenomena is incompletely understood. Here we show that the PIFs and the core clock component Timing of CAB expression 1 (TOC1) display coincident cobinding to the promoters of predawn-phased, growth-related genes under SD conditions. TOC1 interacts with the PIFs and represses their transcriptional activation activity, antagonizing PIF-induced growth. Given the dynamics of TOC1 abundance (displaying high postdusk levels that progressively decline during the long night), our data suggest that TOC1 functions to provide a direct output from the core clock that transiently constrains the growth-promoting activity of the accumulating PIFs early postdusk, thereby gating growth to predawn, when conditions for cell elongation are optimal. These findings unveil a previously unrecognized mechanism whereby a core circadian clock output signal converges immediately with the phytochrome photosensory pathway to coregulate directly the activity of the PIF transcription factors positioned at the apex of a transcriptional network that regulates a diversity of downstream morphogenic responses. C1 [Soy, Judit; Leivar, Pablo; Gonzalez-Schain, Nahuel; Martin, Guiomar; Diaz, Celine; Sentandreu, Maria; Monte, Elena] CSIC, CRAG, Barcelona 08193, Spain. [Leivar, Pablo] Sarria Sch Engn, Inst Quim, Bioengn Dept, Barcelona 08017, Spain. [Al-Sady, Bassem; Quail, Peter H.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Al-Sady, Bassem; Quail, Peter H.] ARS, Plant Gene Express Ctr, USDA, Albany, CA 94710 USA. [Gonzalez-Schain, Nahuel] Univ Nacl Rosario, Consejo Nacl Invest Cient & Tecn, Inst Biol Mol & Celular Rosario, RA-2000 Rosario, Santa Fe, Argentina. [Diaz, Celine] Dadelos Agrosolut, Valencia 46020, Spain. [Sentandreu, Maria] Univ Geneva, Dept Bot & Plant Biol, CH-1211 Geneva, Switzerland. [Al-Sady, Bassem] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA. RP Monte, E (reprint author), CSIC, CRAG, Barcelona 08193, Spain.; Quail, PH (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA.; Quail, PH (reprint author), ARS, Plant Gene Express Ctr, USDA, Albany, CA 94710 USA. EM quail@berkeley.edu; elena.monte@cragenomica.es RI Elena, Monte/I-7221-2015; Leivar, Pablo/D-7808-2017; OI Leivar, Pablo/0000-0003-4878-3684; Martin Matas, Guiomar/0000-0001-6642-1666 FU Spanish Ministerio de Ciencia e Innovacion [BIO2009-07675]; Ministerio de Economia [BIO2012-31672]; Generalitat de Catalunya [2009-SGR-206, 2014-SGR-00268]; Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa Fellowship from the Generalitat de Catalunya (Beatriu de Pinos program); Marie Curie International Reintegration [PIRG06-GA-2009-256420]; Marie Curie Career Integration [10-GA-2011-304008]; NIH [2R01 GM-047475]; US Department of Agriculture Agricultural Research Service Current Research Information System [5335-21000-032-00D]; Junta para la Ampliacion de Estudios Predoctoral Fellowship; Postdoctoral Fellowship from the Center for Research in Agricultural Genomics FX We thank A. Pfeiffer for early sharing of data and P. Mas for materials and comments on the manuscript. This work was supported by Spanish Ministerio de Ciencia e Innovacion Grant BIO2009-07675, Ministerio de Economia Grant BIO2012-31672, and Generalitat de Catalunya Grant 2009-SGR-206 (to E.M.); by a Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa Fellowship from the Generalitat de Catalunya (Beatriu de Pinos program) and by Marie Curie International Reintegration Grant PIRG06-GA-2009-256420 and Generalitat de Catalunya Grant 2014-SGR-00268 (to P.L.); by Marie Curie Career Integration Grant 10-GA-2011-304008 (to C.D.); and by NIH Grant 2R01 GM-047475 and US Department of Agriculture Agricultural Research Service Current Research Information System Grant 5335-21000-032-00D (to P.H.Q.). J.S. was supported by a Junta para la Ampliacion de Estudios Predoctoral Fellowship, and C.D. was supported by a Postdoctoral Fellowship from the Center for Research in Agricultural Genomics. NR 32 TC 8 Z9 8 U1 9 U2 15 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 26 PY 2016 VL 113 IS 17 BP 4870 EP 4875 DI 10.1073/pnas.1603745113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK2MK UT WOS:000374748400077 PM 27071129 ER PT J AU Hammond, KR O'Keefe, JM Aldrich, SP Loeb, SC AF Hammond, Kristina R. O'Keefe, Joy M. Aldrich, Stephen P. Loeb, Susan C. TI A Presence-Only Model of Suitable Roosting Habitat for the Endangered Indiana Bat in the Southern Appalachians SO PLOS ONE LA English DT Article ID MYOTIS-SODALIS; CLIMATE-CHANGE; NORTH-AMERICA; BLACK-HILLS; SELECTION; FORESTS; ECOLOGY; VESPERTILIONIDAE; PERFORMANCE; CHIROPTERA AB We know little about how forest bats, which are cryptic and mobile, use roosts on a landscape scale. For widely distributed species like the endangered Indiana bat Myotis sodalis, identifying landscape-scale roost habitat associations will be important for managing the species in different regions where it occurs. For example, in the southern Appalachian Mountains, USA, M. sodalis roosts are scattered across a heavily forested landscape, which makes protecting individual roosts impractical during large-scale management activities. We created a predictive spatial model of summer roosting habitat to identify important predictors using the presence-only modeling program MaxEnt and an information theoretic approach for model comparison. Two of 26 candidate models together accounted for > 0.93 of AICc weights. Elevation and forest type were top predictors of presence; aspect north/south and distance-to-ridge were also important. The final average best model indicated that 5% of the study area was suitable habitat and 0.5% was optimal. This model matched our field observations that, in the southern Appalachian Mountains, optimal roosting habitat for M. sodalis is near the ridge top in south-facing mixed pine-hardwood forests at elevations from 260-575 m. Our findings, coupled with data from other studies, suggest M. sodalis is flexible in roost habitat selection across different ecoregions with varying topography and land use patterns. We caution that, while mature pine-hardwood forests are important now, specific areas of suitable and optimal habitat will change over time. Combining the information theoretic approach with presence-only models makes it possible to develop landscape-scale habitat suitability maps for forest bats. C1 [Hammond, Kristina R.; O'Keefe, Joy M.] Indiana State Univ, Ctr Bat Res Outreach & Conservat, Terre Haute, IN 47809 USA. [Aldrich, Stephen P.] Indiana State Univ, Dept Earth & Environm Syst, Terre Haute, IN 47809 USA. [Loeb, Susan C.] US Forest Serv, USDA, Southern Res Stn, Clemson, SC USA. RP O'Keefe, JM (reprint author), Indiana State Univ, Ctr Bat Res Outreach & Conservat, Terre Haute, IN 47809 USA. EM joyokeefe@gmail.com FU Joint Fire Science Program [09-1-08-2]; USDA Forest Service; Indiana Space Grant Consortium; Tallassee Fund; National Park Service; North Carolina Wildlife Resources Commission; Tennessee Wildlife Resources Agency; Eastern Band of Cherokee Indians FX Funding for this project was provided by the Joint Fire Science Program (09-1-08-2), USDA Forest Service, Indiana Space Grant Consortium, the Tallassee Fund, the National Park Service, the North Carolina Wildlife Resources Commission, Tennessee Wildlife Resources Agency, and the Eastern Band of Cherokee Indians. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 0 Z9 0 U1 8 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 26 PY 2016 VL 11 IS 4 AR e0154464 DI 10.1371/journal.pone.0154464 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK5QB UT WOS:000374973600069 PM 27115605 ER PT J AU Pepin, KM VerCauteren, KC AF Pepin, Kim M. VerCauteren, Kurt C. TI Disease-emergence dynamics and control in a socially-structured wildlife species SO SCIENTIFIC REPORTS LA English DT Article ID CLASSICAL SWINE-FEVER; FACIAL TUMOR DISEASE; BOAR SUS-SCROFA; MOUTH-DISEASE; CONTACT NETWORKS; INFECTIOUS-DISEASE; TRANSMISSION; PIGS; POPULATION; MODELS AB Once a pathogen is introduced in a population, key factors governing rate of spread include contact structure, supply of susceptible individuals and pathogen life-history. We examined the interplay of these factors on emergence dynamics and efficacy of disease prevention and response. We contrasted transmission dynamics of livestock viruses with different life-histories in hypothetical populations of feral swine with different contact structures (homogenous, metapopulation, spatial and network). Persistence probability was near 0 for the FMDV-like case under a wide range of parameter values and contact structures, while persistence was probable for the CSFV-like case. There were no sets of conditions where the FMDV-like pathogen persisted in every stochastic simulation. Even when population growth rates were up to 300% annually, the FMDV-like pathogen persisted in <25% of simulations regardless of transmission probabilities and contact structure. For networks and spatial contact structure, persistence probability of the FMDV-like pathogen was always <10%. Because of its low persistence probability, even very early response to the FMDV-like pathogen in feral swine was unwarranted while response to the CSFV-like pathogen was generally effective. When pre-emergence culling of feral swine caused population declines, it was effective at decreasing outbreak size of both diseases by >= 80%. C1 [Pepin, Kim M.; VerCauteren, Kurt C.] USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, 4101 Laporte Ave, Ft Collins, CO 80521 USA. RP Pepin, KM (reprint author), USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Wildlife Serv, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM kim.m.pepin@aphis.usda.gov FU USDA APHIS National Feral Swine Damage Management Program FX The work was funded by the USDA APHIS National Feral Swine Damage Management Program. NR 51 TC 0 Z9 0 U1 9 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD APR 26 PY 2016 VL 6 AR 25150 DI 10.1038/srep25150 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DK6WF UT WOS:000375065600001 PM 27114031 ER PT J AU Karchesy, YM Kelsey, RG Constantine, G Karchesy, JJ AF Karchesy, Yvette M. Kelsey, Rick G. Constantine, George Karchesy, Joseph J. TI Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay SO SPRINGERPLUS LA English DT Article DE Brine shrimp lethality; Artemia salina; Methanol extracts; Bioactivity ID IXODES-SCAPULARIS ACARI; MEDICINAL-PLANTS; ESSENTIAL OIL; ANTIMICROBIAL ACTIVITY; PHYTOPHTHORA-RAMORUM; NATURAL-PRODUCTS; YELLOW-CEDAR; HEARTWOOD; IXODIDAE; CONSTITUENTS AB The brine shrimp (Artemia salina) bioassay was used to screen 211 methanol extracts from 128 species of Pacific Northwest plants in search of general cytotoxic activity. Strong toxicity (LC50 < 100 mu g/ml) was found for 17 extracts from 13 species, with highest activity observed for Angelica arguta roots at < 10 mu g/ml. Notably, four species of cedar trees and one of juniper in the family Cupressaceae dominated this group with LC50 for heartwood extracts ranging from 15 to 89 mu g/ml. Moderate toxicity (LC50 100-500 mu g/ml) was found in 38 extracts from 27 species, while weak toxicity (LC50 500-1000 mu g/ml) was detected for 17 extracts in 16 species. There were 139 extracts from 99 species that were non-toxic (LC50 > 1000 mu g/ml). Our subsequent studies of conifer heartwoods with strong activity confirm the assay's value for identifying new investigational leads for materials with insecticidal and fungicidal activity. C1 [Karchesy, Yvette M.; Karchesy, Joseph J.] Oregon State Univ, Wood Sci & Engn, Corvallis, OR 97331 USA. [Kelsey, Rick G.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Constantine, George] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. RP Kelsey, RG (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. EM rkelsey@fs.fed.us NR 35 TC 0 Z9 0 U1 3 U2 9 PU SPRINGER INTERNATIONAL PUBLISHING AG PI CHAM PA GEWERBESTRASSE 11, CHAM, CH-6330, SWITZERLAND SN 2193-1801 J9 SPRINGERPLUS JI SpringerPlus PD APR 23 PY 2016 VL 5 AR 510 DI 10.1186/s40064-016-2145-1 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DL5UT UT WOS:000375703200008 PM 27186474 ER PT J AU Brito, BP Jori, F Dwarka, R Maree, FF Heath, L Perez, AM AF Brito, Barbara P. Jori, Ferran Dwarka, Rahana Maree, Francois F. Heath, Livio Perez, Andres M. TI Transmission of Foot-and-Mouth Disease SAT2 Viruses at the Wildlife-Livestock Interface of Two Major Transfrontier Conservation Areas in Southern Africa SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE foot and mouth disease; SAT2; molecular epidemiology; phylogeography; Southern Africa ID KRUGER-NATIONAL-PARK; IMPALA AEPYCEROS-MELAMPUS; SUB-SAHARAN AFRICA; WILDLIFE/LIVESTOCK INTERFACE; MOLECULAR CHARACTERIZATION; RISK; OUTBREAKS; STRAINS; BUFFALO; CATTLE AB Over a decade ago, foot-and-mouth disease (FMD) re-emerged in Southern Africa specifically in beef exporting countries that had successfully maintained disease-free areas in the past. FMD virus (FMDV) serotype SAT2 has been responsible for a majority of these outbreaks. Epidemiological studies have revealed the importance of the African buffalo as the major wildlife FMD reservoir in the region. We used phylogeographic analysis to study dynamics of FMD transmission between buffalo and domestic cattle at the interface of the major wildlife protected areas in the region currently encompassing two largest Transfrontier conservation areas: Kavango Zambezi (KAZA) and Great Limpopo (GL). Results of this study showed restricted local occurrence of each FMDV SAT2 topotypes I, II, and III, with occasional virus migration from KAZA to GL. Origins of outbreaks in livestock are frequently attributed to wild buffalo, but our results suggest that transmission from cattle to buffalo also occurs. VVe used coalescent Bayesian skyline analysis to study the genetic variation of the virus in cattle and buffalo, and discussed the association of these genetic changes in the virus and relevant epidemiological events that occurred in this area. Our results show that the genetic diversity of FMDV SAT2 has decreased in buffalo and cattle population during the last decade. This study contributes to understand the major dynamics of transmission and genetic variation of FMDV SAT2 in Southern Africa, which will could ultimately help in designing efficient strategies for the control of FMD at a local and regional level. C1 [Brito, Barbara P.] ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [Brito, Barbara P.] Univ Chile, Dept Med Prevent Anim, Fac Ciencias Vet & Pecuarias, Santiago, Chile. [Jori, Ferran] French Agr Res Ctr Int Dev CIRAD, Unite Propre Rech Anim & Gest Integree Risques, Montpellier, France. [Jori, Ferran; Maree, Francois F.] Univ Pretoria, Dept Zool & Entomol, ZA-0002 Pretoria, South Africa. [Jori, Ferran] Botswana Coll Agr, Dept Anim Sci & Prod, Gaborone, Botswana. [Dwarka, Rahana; Maree, Francois F.; Heath, Livio] Ondesterpoort Vet Inst, Transboundary Anim Dis Programme, Onderstepoort, South Africa. [Perez, Andres M.] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, Minneapolis, MN 55455 USA. RP Brito, BP (reprint author), ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA.; Brito, BP (reprint author), Univ Chile, Dept Med Prevent Anim, Fac Ciencias Vet & Pecuarias, Santiago, Chile. EM barbara.brito.r@gmail.com FU USDA/ARS; CORUS (French Ministry of Foreign Affairs); SADC FMD Project FX This project was funded in part by grants from the USDA/ARS and CORUS (French Ministry of Foreign Affairs). The FMD virus database was compiled with funds from the SADC FMD Project. The present manuscript wishes to be the authors' personal honor to the memory of Dr. RD, as a tribute to her scientific competence, and the contribution that she gave to our research project in these years of proficient collaboration. NR 55 TC 0 Z9 0 U1 3 U2 3 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 22 PY 2016 VL 7 AR 528 DI 10.3389/fmicb.2016.00528 PG 10 WC Microbiology SC Microbiology GA DK0HY UT WOS:000374595000001 PM 27148217 ER PT J AU Raza, A Malik, HJ Shafiq, M Amin, I Scheffler, JA Scheffler, BE Mansoor, S AF Raza, Amir Malik, Hassan Jamil Shafiq, Muhammad Amin, Imran Scheffler, Jodi A. Scheffler, Brian E. Mansoor, Shahid TI RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly SO PLOS ONE LA English DT Article ID MANAGING RESISTANCE; CROP PROTECTION; PLANTS; GENE; ALEYRODIDAE; EXPRESSION; INSECTS; COTTON; CHALLENGES; PESTS AB Over the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siR-NAs) that could offer great potential for insect pest management. The diet of insects feeding exclusively on phloem sieves contains water and sugars as main components, and the uptake of the liquid food greatly depends on the osmotic pressure within the insect body. Based on this physiological mechanism, transgenic plants of Nicotiana tabacum were generated expressing double stranded RNA (dsRNA) against both aquaporin (AQP) and a sucrase gene, alpha glucosidase (AGLU). These two genes are involved in osmotic pressure maintenance particularly in sap sucking insects, and the aim was to disrupt osmoregulation within the insect ultimately leading to mortality. Real time quantitative PCR (RT-qPCR) was performed to assess the suppression of gene expression in Bemisia tabaci (B. tabaci) and mortality was recorded during transgenic tobacco feeding bioassays. Feeding of insects on plants expressing dsRNA significantly reduced the transcript level of the target genes in B. tabaci after six days of feeding and more than 70% mortality was observed in B. tabaci fed on transgenic plants compared to the control plants. Our data shows that down-regulation of genes related to osmoregulation may find practical applications for the control of this important pest in cotton and other crops. C1 [Raza, Amir; Malik, Hassan Jamil; Shafiq, Muhammad; Amin, Imran; Mansoor, Shahid] NIBGE, Mol Virol & Gene Silencing Lab, Agr Biotechnol Div, Jhang Rd,POB 577, Faisalabad, Pakistan. [Raza, Amir; Malik, Hassan Jamil; Shafiq, Muhammad] PIEAS, Islamabad, Pakistan. [Scheffler, Jodi A.] USDA ARS, Crop Genet Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. [Scheffler, Brian E.] USDA ARS, Genom & Bioinformat Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. RP Mansoor, S (reprint author), NIBGE, Mol Virol & Gene Silencing Lab, Agr Biotechnol Div, Jhang Rd,POB 577, Faisalabad, Pakistan. EM shahidmansoor7@gmail.com OI Scheffler, Brian/0000-0003-1968-8952 FU Higher Education Commission; Pakistan - United States Cotton Productivity Enhancement Program; United States Department of Agriculture Agricultural Research Service [58-6402-0-178F] FX Funding provided by Higher Education Commission, AR, MS Pakistan - United States Cotton Productivity Enhancement Program, United States Department of Agriculture Agricultural Research Service Project No. 58-6402-0-178F. NR 49 TC 5 Z9 5 U1 8 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 22 PY 2016 VL 11 IS 4 AR e0153883 DI 10.1371/journal.pone.0153883 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ9WX UT WOS:000374565100027 PM 27105353 ER PT J AU Lewis, NS Russell, CA Langat, P Anderson, TK Berger, K Bielejec, F Burke, DF Dudas, G Fonville, JM Fouchier, RAM Kellam, P Koel, BF Lemey, P Nguyen, T Nuansrichy, B Peiris, JSM Saito, T Simon, G Skepner, E Takemae, N Webby, RJ Van Reeth, K Brookes, SM Larsen, L Watson, SJ Brown, IH Vincent, AL AF Lewis, Nicola S. Russell, Colin A. Langat, Pinky Anderson, Tavis K. Berger, Kathryn Bielejec, Filip Burke, David F. Dudas, Gytis Fonville, Judith M. Fouchier, Ron A. M. Kellam, Paul Koel, Bjorn F. Lemey, Philippe Tung Nguyen Nuansrichy, Bundit Peiris, J. S. Malik Saito, Takehiko Simon, Gaelle Skepner, Eugene Takemae, Nobuhiro Webby, Richard J. Van Reeth, Kristien Brookes, Sharon M. Larsen, Lars Watson, Simon J. Brown, Ian H. Vincent, Amy L. CA ESNIP3 Consortium TI The global antigenic diversity of swine influenza A viruses SO ELIFE LA English DT Article ID PANDEMIC H1N1 INFLUENZA; BINDING SITE DETERMINE; EURASIAN AVIAN-LIKE; UNITED-STATES; H3N2 VIRUS; GENETIC EVOLUTION; A(H1N1)PDM09 VIRUS; EUROPEAN SWINE; HUMAN-ORIGIN; COUNTY FAIR AB Swine influenza presents a substantial disease burden for pig populations worldwide and poses a potential pandemic threat to humans. There is considerable diversity in both H1 and H3 influenza viruses circulating in swine due to the frequent introductions of viruses from humans and birds coupled with geographic segregation of global swine populations. Much of this diversity is characterized genetically but the antigenic diversity of these viruses is poorly understood. Critically, the antigenic diversity shapes the risk profile of swine influenza viruses in terms of their epizootic and pandemic potential. Here, using the most comprehensive set of swine influenza virus antigenic data compiled to date, we quantify the antigenic diversity of swine influenza viruses on a multi-continental scale. The substantial antigenic diversity of recently circulating viruses in different parts of the world adds complexity to the risk profiles for the movement of swine and the potential for swine-derived infections in humans. C1 [Lewis, Nicola S.; Burke, David F.; Fonville, Judith M.; Skepner, Eugene] Univ Cambridge, Dept Zool, Cambridge, England. [Russell, Colin A.; Berger, Kathryn] Univ Cambridge, Dept Vet Med, Cambridge, England. [Langat, Pinky; Kellam, Paul; Watson, Simon J.] Wellcome Trust Sanger Inst, Hinxton, England. [Anderson, Tavis K.; Vincent, Amy L.] USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Bielejec, Filip; Lemey, Philippe] Katholieke Univ Leuven, Rega Inst Med Res, Dept Microbiol & Immunol, Clin & Epidemiol Virol, Leuven, Belgium. [Dudas, Gytis; Fouchier, Ron A. M.] Univ Edinburgh, Inst Evolutionary Biol, Edinburgh, Midlothian, Scotland. [Koel, Bjorn F.] Erasmus MC, Dept Virosci, Rotterdam, Netherlands. [Tung Nguyen] Natl Ctr Vet Diagnost, Dept Anim Hlth, Hanoi, Vietnam. [Nuansrichy, Bundit] Natl Inst Anim Hlth, Bangkok, Thailand. [Peiris, J. S. Malik] Univ Hong Kong, Sch Publ Hlth, Hong Kong, Hong Kong, Peoples R China. [Saito, Takehiko; Takemae, Nobuhiro] Natl Inst Anim Hlth, Ibaraki, Japan. [Simon, Gaelle] Anses, Ploufragan Plouzane Lab, Swine Virol Immunol Unit, Ploufragan, France. [Webby, Richard J.] St Jude Childrens Res Hosp, 332 N Lauderdale St, Memphis, TN 38105 USA. [Van Reeth, Kristien] Univ Ghent, Fac Vet Med, Virol Lab, B-9000 Ghent, Belgium. [Brookes, Sharon M.; Brown, Ian H.] Anim Hlth & Plant Agcy, Weybridge, Surrey, England. [Larsen, Lars] Tech Univ Denmark, Natl Vet Inst, Frederiksberg, Denmark. [Koel, Bjorn F.] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, NL-1105 AZ Amsterdam, Netherlands. RP Lewis, NS (reprint author), Univ Cambridge, Dept Zool, Cambridge, England. EM nsl25@cam.ac.uk RI APHA, Staff publications/E-6082-2010; Fouchier, Ron/A-1911-2014; OI Fouchier, Ron/0000-0001-8095-2869; burke, david/0000-0001-8830-3951; Russell, Colin/0000-0002-2113-162X; SIMON, Gaelle/0000-0003-1099-5075; Dudas, Gytis/0000-0002-0227-4158; Anderson, Tavis/0000-0002-3138-5535 FU United States Department of Agriculture-ARS [58-3625-2-103F, 58-3625-4-070]; European Union [259949]; Medical Research Council Fellowship [MR/K021885/1]; University Research Fellowship from the Royal Society; DEFRA government [SV34041]; UK devolved government [SV34041] FX United States Department of Agriculture-ARS SCA agreement number 58-3625-2-103F Nicola S Lewis; European Union Seventh Framework Programme 259949 Nicola S Lewis Paul Kellam Gaelle Simon Richard J Webby Kristien Van Reeth Sharon M Brookes Ian H Brown Lars Larsen Simon J Watson Amy L Vincent; Medical Research Council Fellowship MR/K021885/1 Judith M Fonville; United States Department of Agriculture-ARS SCA agreement number 58-3625-4-070 Tavis K Anderson; University Research Fellowship from the Royal Society Colin A Russell; DEFRA and the UK devolved governments SV34041 Sharon M Brookes Ian H Brown; The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 73 TC 3 Z9 3 U1 3 U2 12 PU ELIFE SCIENCES PUBLICATIONS LTD PI CAMBRIDGE PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND SN 2050-084X J9 ELIFE JI eLife PD APR 22 PY 2016 VL 5 AR e12217 DI 10.7554/eLife.12217 PG 17 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DK3NR UT WOS:000374824500001 PM 27113719 ER PT J AU Covarrubias-Pazaran, G Diaz-Garcia, L Schlautman, B Salazar, W Zalapa, J AF Covarrubias-Pazaran, Giovanny Diaz-Garcia, Luis Schlautman, Brandon Salazar, Walter Zalapa, Juan TI Fragman: an R package for fragment analysis SO BMC GENETICS LA English DT Article DE Fragment analysis; Genetic markers; R package; Least squares; Open source software ID SOFTWARE AB Background: Determination of microsatellite lengths or other DNA fragment types is an important initial component of many genetic studies such as mutation detection, linkage and quantitative trait loci (QTL) mapping, genetic diversity, pedigree analysis, and detection of heterozygosity. A handful of commercial and freely available software programs exist for fragment analysis; however, most of them are platform dependent and lack high-throughput applicability. Results: We present the R package Fragman to serve as a freely available and platform independent resource for automatic scoring of DNA fragment lengths diversity panels and biparental populations. The program analyzes DNA fragment lengths generated in Applied Biosystems (R) (ABI) either manually or automatically by providing panels or bins. The package contains additional tools for converting the allele calls to GenAlEx, JoinMap (R) and OneMap software formats mainly used for genetic diversity and generating linkage maps in plant and animal populations. Easy plotting functions and multiplexing friendly capabilities are some of the strengths of this R package. Fragment analysis using a unique set of cranberry (Vaccinium macrocarpon) genotypes based on microsatellite markers is used to highlight the capabilities of Fragman. Conclusion: Fragman is a valuable new tool for genetic analysis. The package produces equivalent results to other popular software for fragment analysis while possessing unique advantages and the possibility of automation for high-throughput experiments by exploiting the power of R. C1 [Covarrubias-Pazaran, Giovanny; Diaz-Garcia, Luis; Schlautman, Brandon; Salazar, Walter; Zalapa, Juan] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Diaz-Garcia, Luis] Inst Nacl Invest Forestales & Agr Pecuarias, Campo Expt Pabellon, Aguascalientes, Mexico. [Zalapa, Juan] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Covarrubias-Pazaran, G; Zalapa, J (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Zalapa, J (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. EM cova_ruber@live.com.mx; jezalapa@wisc.edu OI COVARRUBIAS-PAZARAN, GIOVANNY/0000-0002-7194-3837 FU USDA-ARS [3655-21220-001-00]; WI-DATCP (SCBG) [14-002]; National Science Foundation [DBI-1228280]; Ocean Spray Cranberries, Inc.; Wisconsin Cranberry Growers Association; Cranberry Institute; Frank B. Koller Cranberry Fellowship Fund for Graduate Students; Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico) FX This project was supported by USDA-ARS (project no. 3655-21220-001-00 provided to JZ and SS); WI-DATCP (SCBG Project #14-002); National Science Foundation (DBI-1228280); Ocean Spray Cranberries, Inc.; Wisconsin Cranberry Growers Association; Cranberry Institute; BS was supported by the Frank B. Koller Cranberry Fellowship Fund for Graduate Students; GCP and LDG were supported by the Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico). NR 17 TC 0 Z9 0 U1 3 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD APR 21 PY 2016 VL 17 AR 62 DI 10.1186/s12863-016-0365-6 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA DJ9TJ UT WOS:000374555500001 PM 27098093 ER PT J AU Wang, Y Liu, XJ Ren, C Zhong, GY Yang, L Li, SH Liang, ZC AF Wang, Yi Liu, Xianju Ren, Chong Zhong, Gan-Yuan Yang, Long Li, Shaohua Liang, Zhenchang TI Identification of genomic sites for CRISPR/Cas9-based genome editing in the Vitis vinifera genome SO BMC PLANT BIOLOGY LA English DT Article DE CRISPR/Cas9; Database; Genome editing; PAM; Vitis vinifera ID RNA; PLANTS; ENDONUCLEASE; ARABIDOPSIS; SEQUENCE; SYSTEMS; CAS9; TOOL AB Background: CRISPR/Cas9 has been recently demonstrated as an effective and popular genome editing tool for modifying genomes of humans, animals, microorganisms, and plants. Success of such genome editing is highly dependent on the availability of suitable target sites in the genomes to be edited. Many specific target sites for CRISPR/Cas9 have been computationally identified for several annual model and crop species, but such sites have not been reported for perennial, woody fruit species. In this study, we identified and characterized five types of CRISPR/Cas9 target sites in the widely cultivated grape species Vitis vinifera and developed a user-friendly database for editing grape genomes in the future. Results: A total of 35,767,960 potential CRISPR/Cas9 target sites were identified from grape genomes in this study. Among them, 22,597,817 target sites were mapped to specific genomic locations and 7,269,788 were found to be highly specific. Protospacers and PAMs were found to distribute uniformly and abundantly in the grape genomes. They were present in all the structural elements of genes with the coding region having the highest abundance. Five PAM types, TGG, AGG, GGG, CGG and NGG, were observed. With the exception of the NGG type, they were abundantly present in the grape genomes. Synteny analysis of similar genes revealed that the synteny of protospacers matched the synteny of homologous genes. A user-friendly database containing protospacers and detailed information of the sites was developed and is available for public use at the Grape-CRISPR website (http://biodb.sdau.edu.cn/gc/index.html). Conclusion: Grape genomes harbour millions of potential CRISPR/Cas9 target sites. These sites are widely distributed among and within chromosomes with predominant abundance in the coding regions of genes. We developed a publicly-accessible Grape-CRISPR database for facilitating the use of the CRISPR/Cas9 system as a genome editing tool for functional studies and molecular breeding of grapes. Among other functions, the database allows users to identify and select multi-protospacers for editing similar sequences in grape genomes simultaneously. C1 [Wang, Yi; Liu, Xianju; Ren, Chong; Li, Shaohua; Liang, Zhenchang] Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China. [Wang, Yi; Liu, Xianju; Ren, Chong; Li, Shaohua; Liang, Zhenchang] Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China. [Liu, Xianju] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Zhong, Gan-Yuan] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14456 USA. [Yang, Long] Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China. [Liang, Zhenchang] Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China. RP Li, SH (reprint author), Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China.; Li, SH (reprint author), Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China. EM shhli@ibcas.ac.cn FU National Natural Science Foundation of China [31572090]; Hundred Talents of Chinese Academy of Sciences FX We thank Dr. D.D. Archbold (Professor, University of Kentucky, USA) for his English improvement of the manuscript. This study was financially supported by National Natural Science Foundation of China (31572090) and Hundred Talents of Chinese Academy of Sciences. NR 16 TC 2 Z9 2 U1 11 U2 27 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD APR 21 PY 2016 VL 16 AR 96 DI 10.1186/s12870-016-0787-3 PG 7 WC Plant Sciences SC Plant Sciences GA DJ9HY UT WOS:000374525800002 PM 27098585 ER PT J AU Baranzoni, GM Fratamico, PM Gangiredla, J Patel, I Bagi, LK Delannoy, S Fach, P Boccia, F Anastasio, A Pepe, T AF Baranzoni, Gian Marco Fratamico, Pina M. Gangiredla, Jayanthi Patel, Isha Bagi, Lori K. Delannoy, Sabine Fach, Patrick Boccia, Federica Anastasio, Aniello Pepe, Tiziana TI Characterization of Shiga Toxin Subtypes and Virulence Genes in Porcine Shiga Toxin-Producing Escherichia coil SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Escherichia coli; STEC; swine; Shiga toxins variants; virulence genes ID HEMOLYTIC-UREMIC-SYNDROME; COLI STRAINS; ENTEROCYTE EFFACEMENT; MOLECULAR ANALYSIS; BIOFILM FORMATION; UNITED-STATES; FECAL SAMPLES; SWINE FECES; GROUND-BEEF; NON-O157 AB Similar to ruminants, swine have been shown to be a reservoir for Shiga toxin-producing Escherichia coil (STEC), and pork products have been linked with outbreaks associated with STEC 0157 and 0111:H-. STEC strains, isolated in a previous study from fecal samples of late-finisher pigs, belonged to a total of 56 serotypes, including 015:H27, 091:H14, and other serogroups previously associated with human illness. The isolates were tested by polymerase chain reaction (PCR) and a high-throughput real-time PCR system to determine the Shiga toxin (Stx) subtype and virulence-associated and putative virulence-associated genes they carried. Select STEC strains were further analyzed using a Minimal Signature E. coil Array Strip. As expected, stx(2e) (81%) was the most common Stx variant, followed by stx(1a) (14%), stx(2d) (3%), and stx(1c) (1%). The STEC serogroups that carried stx(2d) were 015:H27, 0159:H16 and 0159:H-. Similar to stx(2a) and stx(2c), the stx(2d) variant is associated with development of hemorrhagic colitis and hemolytic uremic syndrome, and reports on the presence of this variant in STEC strains isolated from swine are lacking. Moreover, the genes encoding heat stable toxin (estla) and enteroaggregative coil heat stable enterotoxin-1 (astA) were commonly found in 50 and 44% of isolates, respectively. The hemolysin genes, hlyA and ehxA, were both detected in 7% of the swine STEC strains. Although the eae gene was not found, other genes involved in host cell adhesion, including IpfA(O113) and paa were detected in more than 50% of swine STEC strains, and a number of strains also carried iha, 1PfA(O26), 1pfA(O157), fedA, orfA, and orfB. The present work provides new insights on the distribution of virulence factors among swine STEC strains and shows that swine may carry Stxla-, Stx2e-, or Stx2d-producing E, coil with virulence gene profiles associated with human infections. C1 [Baranzoni, Gian Marco; Fratamico, Pina M.; Bagi, Lori K.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Gangiredla, Jayanthi; Patel, Isha] US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD USA. [Delannoy, Sabine; Fach, Patrick] Univ Paris Est, Food Safety Lab, Maisons Alfort, France. [Boccia, Federica; Anastasio, Aniello; Pepe, Tiziana] Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, Italy. RP Fratamico, PM (reprint author), USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. EM pina.fratamico@ars.usda.gov FU Agricultural Research services (ARS) Research Participation Program; ORAU under DOE [DE-AC05-06OR23100]; French joint ministerial program of R&D against CBRNE risks FX This research was supported in part by an appointment to the Agricultural Research services (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the USDA. ORISE is managed by ORAU under DOE contract number DE-AC05-06OR23100. The hrPCR development was partially financed by the French joint ministerial program of R&D against CBRNE risks. All opinions expressed in this manuscript are the authors' and do not necessarily reflect the policies and views of USDA, ARS, DOE, or ORAU/ORISE. NR 75 TC 2 Z9 2 U1 1 U2 8 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 21 PY 2016 VL 7 AR 574 DI 10.3389/fmich.2016.00574 PG 10 WC Microbiology SC Microbiology GA DJ8ON UT WOS:000374471800001 PM 27148249 ER PT J AU Gazave, E Tassone, EE Ilut, DC Wingerson, M Datema, E Witsenboer, HMA Davis, JB Grant, D Dyer, JM Jenks, MA Brown, J Gore, MA AF Gazave, Elodie Tassone, Erica E. Ilut, Daniel C. Wingerson, Megan Datema, Erwin Witsenboer, Hanneke M. A. Davis, James B. Grant, David Dyer, John M. Jenks, Matthew A. Brown, Jack Gore, Michael A. TI Population Genomic Analysis Reveals Differential Evolutionary Histories and Patterns of Diversity across Subgenomes and Subpopulations of Brassica napus L. SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Brassica napus; sequence-based genotyping; diversity panel; population differentiation; nucleotide diversity; site frequency spectrum; phylogenetic tree; inversion polymorphism ID DNA-SEQUENCING DATA; GENETIC DIVERSITY; LOCAL ADAPTATION; RAPESEED; CANOLA; INVERSIONS; SELECTION; VARIANTS; POLYMORPHISM; BOTTLENECKS AB The allotetraploid species Brassica napus L. is a global crop of major economic importance, providing canola oil (seed) and vegetables for human consumption and fodder and meal for livestock feed. Characterizing the genetic diversity present in the extant germplasm pool of B. napus is fundamental to better conserve, manage and utilize the genetic resources of this species. We used sequence-based genotyping to identify and genotype 30,881 SNPs in a diversity panel of 782 B. napus accessions, representing samples of winter and spring growth habits originating from 33 countries across Europe, Asia, and America. We detected strong population structure broadly concordant with growth habit and geography, and identified three major genetic groups: spring (SP), winter Europe (WE), and winter Asia (WA). Subpopulation-specific polymorphism patterns suggest enriched genetic diversity within the WA group and a smaller effective breeding population for the SP group compared to WE. Interestingly, the two subgenomes of B. napus appear to have different geographic origins, with phylogenetic analysis placing WE and WA as basal clades for the other subpopulations in the C and A subgenomes, respectively. Finally, we identified 16 genomic regions where the patterns of diversity differed markedly from the genome-wide average, several of which are suggestive of genomic inversions. The results obtained in this study constitute a valuable resource for worldwide breeding efforts and the genetic dissection and prediction of complex B. napus traits. C1 [Gazave, Elodie; Ilut, Daniel C.; Gore, Michael A.] Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY USA. [Tassone, Erica E.; Dyer, John M.] USDA ARS, Plant Physiol & Genet Res Unit, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Wingerson, Megan; Davis, James B.; Brown, Jack] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA. [Datema, Erwin; Witsenboer, Hanneke M. A.] Keygene NV, Wageningen, Netherlands. [Grant, David] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. [Jenks, Matthew A.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. RP Gazave, E; Gore, MA (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Breeding & Genet Sect, Ithaca, NY USA. EM eeg47@cornell.edu; mag87@cornell.edu FU USDA-ARS; USDA-NIFA/DOE Biomass Research and Development Initiative (BRIM) [2011-06476, 2012-10006]; Cornell University startup funds FX This work is funded and supported by the USDA-ARS, USDA-NIFA/DOE Biomass Research and Development Initiative (BRIM) Proposals Nos. 2011-06476 and 2012-10006, and Cornell University startup funds. NR 66 TC 0 Z9 0 U1 11 U2 22 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD APR 21 PY 2016 VL 7 AR 525 DI 10.3389/fpls.2015.00525 PG 16 WC Plant Sciences SC Plant Sciences GA DJ9PE UT WOS:000374544600001 PM 27148342 ER PT J AU Syring, JV Tennessen, JA Jennings, TN Wegrzyn, J Scelfo-Dalbey, C Cronn, R AF Syring, John V. Tennessen, Jacob A. Jennings, Tara N. Wegrzyn, Jill Scelfo-Dalbey, Camille Cronn, Richard TI Targeted Capture Sequencing in Whitebark Pine Reveals Range-Wide Demographic and Adaptive Patterns Despite Challenges of a Large, Repetitive Genome SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Pinus albicaulis; whitebark pine; gene conservation; target enrichment; SNP; landscape genomics ID GENETIC-STRUCTURE; ENRICHMENT STRATEGIES; CONSERVATION GENETICS; ALBICAULIS FORESTS; NORTH-AMERICA; ALIGNMENT; SEED; EVOLUTIONARY; CONSEQUENCES; POPULATIONS AB Whitebark pine (Pinus albicaulis) inhabits an expansive range in western North America, and it is a keystone species of subalpine environments. Whitebark is susceptible to multiple threats - climate change, white pine blister rust, mountain pine beetle, and fire exclusion - and it is suffering significant mortality range-wide, prompting the tree to be listed as `globally endangered' by the International Union for Conservation of Nature and `endangered' by the Canadian government. Conservation collections (in situ and ex situ) are being initiated to preserve the genetic legacy of the species. Reliable, transferrable, and highly variable genetic markers are essential for quantifying the genetic profiles of seed collections relative to natural stands, and ensuring the completeness of conservation collections. We evaluated the use of hybridization-based target capture to enrich specific genomic regions from the 27 GB genome of whitebark pine, and to evaluate genetic variation across loci, trees, and geography. Probes were designed to capture 7,849 distinct genes, and screening was performed on 48 trees. Despite the inclusion of repetitive elements in the probe pool, the resulting dataset provided information on 4,452 genes and 32% of targeted positions (528,873 bp), and we were able to identify 12,390 segregating sites from 47 trees. Variations reveal strong geographic trends in heterozygosity and allelic richness, with trees from the southern Cascade and Sierra Range showing the greatest distinctiveness and differentiation. Our results show that even under non-optimal conditions (low enrichment efficiency; inclusion of repetitive elements in baits), targeted enrichment produces high quality, codominant genotypes from large genomes. The resulting data can be readily integrated into management and gene conservation activities for whitebark pine, and have the potential to be applied to other members of 5-needle pine group (Pious subsect. Quinquefolia) due to their limited genetic divergence. C1 [Syring, John V.] Linfield Coll, Dept Biol, Mcminnville, OR USA. [Tennessen, Jacob A.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Jennings, Tara N.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Wegrzyn, Jill] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT USA. [Scelfo-Dalbey, Camille] Univ Calif Santa Cruz, Jack Baskin Sch Engn, Santa Cruz, CA 95064 USA. [Cronn, Richard] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP Syring, JV (reprint author), Linfield Coll, Dept Biol, Mcminnville, OR USA. EM jsyring@linfield.edu FU MJ Murdock Charitable Trust; Linfield College; Forest Health Protection program of the US Forest Service Pacific Northwest Region Six (Portland, OR, USA) FX Funding for this study was provided by the MJ Murdock Charitable Trust, Linfield College, and the Forest Health Protection program of the US Forest Service Pacific Northwest Region Six (Portland, OR, USA). NR 69 TC 2 Z9 2 U1 11 U2 26 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD APR 21 PY 2016 VL 7 AR 484 DI 10.3380/fpls.2016.00484 PG 15 WC Plant Sciences SC Plant Sciences GA DJ9OT UT WOS:000374543500001 PM 27148310 ER PT J AU Duan, K Sun, G Sun, SL Caldwell, PV Cohen, EC McNulty, SG Aldridge, HD Zhang, Y AF Duan, Kai Sun, Ge Sun, Shanlei Caldwell, Peter V. Cohen, Erika C. McNulty, Steven G. Aldridge, Heather D. Zhang, Yang TI Divergence of ecosystem services in US National Forests and Grasslands under a changing climate SO SCIENTIFIC REPORTS LA English DT Article ID CONTERMINOUS UNITED-STATES; CHANGE IMPACTS; WATER; EVAPOTRANSPIRATION; MODEL; UNCERTAINTY; MANAGEMENT AB a The 170 National Forests and Grasslands (NFs) in the conterminous United States are public lands that provide important ecosystem services such as clean water and timber supply to the American people. This study investigates the potential impacts of climate change on two key ecosystem functions (i.e., water yield and ecosystem productivity) using the most recent climate projections derived from 20 Global Climate Models (GCMs) of the Coupled Model Intercomparison Project phase 5 (CMIP5). We find that future climate change may result in a significant reduction in water yield but an increase in ecosystem productivity in NFs. On average, gross ecosystem productivity is projected to increase by 76 similar to 229 g C m(-2) yr(-1) (8% similar to 24%) while water yield is projected to decrease by 18 similar to 31 mm yr-1 (4% similar to 7%) by 2100 as a result of the combination of increased air temperature (+1.8 similar to + 52 degrees C) and precipitation (+17 similar to +51 mm yr(-1)). The notable divergence in ecosystem services of water supply and carbon sequestration is expected to intensify under higher greenhouse gas emission and associated climate change in the future, posing greater challenges to managing NFs for both ecosystem services. C1 [Duan, Kai; Zhang, Yang] N Carolina State Univ, Dept Marine Earth & Atmospher Sci, Box 8208, Raleigh, NC 27695 USA. [Sun, Ge; Cohen, Erika C.; McNulty, Steven G.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC USA. [Sun, Shanlei] Nanjing Univ Informat Sci & Technol, Key Lab Meteorol Disaster, Minist Educ, Nanjing, Jiangsu, Peoples R China. [Caldwell, Peter V.] US Forest Serv, Coweeta Hydrol Lab, USDA, Otto, NC USA. [Aldridge, Heather D.] N Carolina State Univ, State Climate Off North Carolina, Raleigh, NC 27695 USA. RP Sun, G (reprint author), US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC USA. EM gesun@fs.fed.us FU National Science Foundation EaSM program [AGS-1049200]; Eastern Forest Environmental Threat Assessment Center (EFETAC), USDA Forest Service; Northwest Climate Science Center (NW CSC) US Geological Survey [G12AC20495]; Pine Integrated Network: Education, Mitigation, and Adaptation project (PINEMAP); USDA National Institute of Food and Agriculture [2011-68002-30185] FX This work was supported by the National Science Foundation EaSM program (AGS-1049200) awarded to North Carolina State University, and the Eastern Forest Environmental Threat Assessment Center (EFETAC), USDA Forest Service. The MACAv2-LIVNEH dataset was produced under the Northwest Climate Science Center (NW CSC) US Geological Survey Grant Number G12AC20495. Partial support was provided by the Pine Integrated Network: Education, Mitigation, and Adaptation project (PINEMAP), which is a Coordinated Agricultural Project funded by the USDA National Institute of Food and Agriculture, Award #2011-68002-30185. NR 47 TC 1 Z9 2 U1 12 U2 25 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD APR 21 PY 2016 VL 6 AR 24441 DI 10.1038/srep24441 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ8SU UT WOS:000374484100001 PM 27100360 ER PT J AU Lin, T Wang, SH Zhong, Y Gao, DL Cui, QZ Chen, HM Zhang, ZH Shen, HL Weng, YQ Huang, SW AF Lin, Tao Wang, Shenhao Zhong, Yang Gao, Dongli Cui, Qingzhi Chen, Huiming Zhang, Zhonghua Shen, Huolin Weng, Yiqun Huang, Sanwen TI A Truncated F-Box Protein Confers the Dwarfism in Cucumber SO JOURNAL OF GENETICS AND GENOMICS LA English DT Letter ID GREEN-REVOLUTION; SATIVUS L; INHERITANCE; MUTANT; GENES; REVEALS; GENOME; RICE; L. C1 [Lin, Tao; Wang, Shenhao; Zhong, Yang; Gao, Dongli; Zhang, Zhonghua; Huang, Sanwen] Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Sino Dutch Joint Lab Hort Genom, Inst Vegetables & Flowers,Minist Agr, Beijing 100081, Peoples R China. [Lin, Tao; Shen, Huolin] China Agr Univ, Dept Hort, Beijing 100094, Peoples R China. [Lin, Tao; Gao, Dongli; Huang, Sanwen] Chinese Acad Agr Sci, Agr Genome Inst Shenzhen, Shenzhen 518124, Peoples R China. [Cui, Qingzhi; Chen, Huiming] Hunan Acad Agr Sci, Hunan Vegetable Res Inst, Changsha 410125, Hunan, Peoples R China. [Weng, Yiqun] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Dept Hort, Madison, WI 53706 USA. RP Huang, SW (reprint author), Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, Sino Dutch Joint Lab Hort Genom, Inst Vegetables & Flowers,Minist Agr, Beijing 100081, Peoples R China.; Huang, SW (reprint author), Chinese Acad Agr Sci, Agr Genome Inst Shenzhen, Shenzhen 518124, Peoples R China. EM huangsanwen@caas.cn NR 21 TC 0 Z9 0 U1 4 U2 5 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1673-8527 EI 1873-5533 J9 J GENET GENOMICS JI J. Genet. Genomics PD APR 20 PY 2016 VL 43 IS 4 BP 223 EP 226 DI 10.1016/j.jgg.2016.01.007 PG 4 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA DL5BT UT WOS:000375652600007 PM 27090606 ER PT J AU Bouskill, NJ Wood, TE Baran, R Ye, Z Bowen, BP Lim, HC Zhou, JZ Van Nostrand, JD Nico, P Northen, TR Silver, WL Brodie, EL AF Bouskill, Nicholas J. Wood, Tana E. Baran, Richard Ye, Zaw Bowen, Benjamin P. Lim, Hsiao Chien Zhou, Jizhong Van Nostrand, Joy D. Nico, Peter Northen, Trent R. Silver, Whendee L. Brodie, Eoin L. TI Belowground Response to Drought in a Tropical Forest Soil. I. Changes in Microbial Functional Potential and Metabolism SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE tropical forests; drought; microbial ecology; osmolytes; functional gene microarray ID CLIMATE-CHANGE; ENZYME-ACTIVITY; EXTRACELLULAR ENZYMES; BACTERIAL COMMUNITIES; LITTER DECOMPOSITION; CALIFORNIA GRASSLAND; ORGANIC-CARBON; STRESS; MICROORGANISMS; MOISTURE AB Global climate models predict a future of increased severity of drought in many tropical forests. Soil microbes are central to the balance of these systems as sources or sinks of atmospheric carbon (C), yet how they respond metabolically to drought is not well understood. We simulated drought in the typically aseasonal Luquillo Experimental Forest, Puerto Rico, by intercepting precipitation falling through the forest canopy. This approach reduced soil moisture by 13% and water potential by 0.14 MPa (from -0.2 to -0.34). Previous results from this experiment have demonstrated that the diversity and composition of these soil microbial communities are sensitive to even small changes in soil water. Here, we show prolonged drought significantly alters the functional potential of the community and provokes a clear osmotic stress response, including the production of compatible solutes that increase intracellular C demand. Subsequently, a microbial population emerges with a greater capacity for extracellular enzyme production targeting macromolecular carbon. Significantly, some of these drought-induced functional shifts in the soil microbiota are attenuated by prior exposure to a short-term drought suggesting that acclimation may occur despite a lack of longer-term drought history. C1 [Bouskill, Nicholas J.; Ye, Zaw; Lim, Hsiao Chien; Zhou, Jizhong; Nico, Peter; Brodie, Eoin L.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Dept Ecol, Div Earth Sci, Berkeley, CA 94720 USA. [Wood, Tana E.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR USA. [Wood, Tana E.] Fdn Puertorriquena Conservac, San Juan, PR USA. [Baran, Richard; Bowen, Benjamin P.; Northen, Trent R.] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA. [Zhou, Jizhong; Van Nostrand, Joy D.] Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA. [Zhou, Jizhong; Van Nostrand, Joy D.] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Zhou, Jizhong] Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China. [Silver, Whendee L.; Brodie, Eoin L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Bouskill, NJ; Brodie, EL (reprint author), Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Dept Ecol, Div Earth Sci, Berkeley, CA 94720 USA.; Brodie, EL (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM njbouskill@lbl.gov; elbrodie@lbl.gov RI Brodie, Eoin/A-7853-2008; Bouskill, Nick/G-2390-2015; Nico, Peter/F-6997-2010; OI Brodie, Eoin/0000-0002-8453-8435; Nico, Peter/0000-0002-4180-9397; Northen, Trent/0000-0001-8404-3259 FU Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory; U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-050.111231, DE-AC02-05CH11231]; NSF [EAR-08199072, DEB 0620910]; Luquillo Critical Zone Observatory [EAR-0722476]; USGS Luquillo WEBB program FX This work was partially supported by the Laboratory Directed Research and Development Program of Lawrence Berkeley National Laboratory. The Subsurface Biogeochemical Research program, the Next Generation Ecosystem Experiment (NGEE-Tropics), and ENIGMA-Ecosystems :and Networks Integrated with Genes and Molecular Assemblies (http://enigma.lbl.gov). All Scientific Focus Area Programs at Lawrence Berkeley National Laboratory supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research under contract number DE-AC02-050.111231. This work was also partially funded by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research under contract number DE-AC02-05CH11231, and by NSF grant EAR-08199072 to WS, the Luquillo Critical Zone Observatory (EAR-0722476) with additional support provided by the USGS Luquillo WEBB program, and grant DEB 0620910 from NSF to the Institute of Tropical Forest Ecosystem Studies, University of Puerto Rico, and to the International Institute of Tropical Forestry USDA Forest Service, as part of the Luquillo Long-Term Ecological Research Program. NR 87 TC 2 Z9 2 U1 23 U2 57 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 20 PY 2016 VL 7 AR 525 DI 10.3389/fmicb.2016.00525 PG 11 WC Microbiology SC Microbiology GA DJ7DO UT WOS:000374372300001 PM 27148214 ER PT J AU Zhang, LL Liu, RQ Gung, BW Tindall, S Gonzalez, JM Halvorson, JJ Hagerman, AE AF Zhang, Liangliang Liu, Ruiqiang Gung, Benjamin W. Tindall, Steven Gonzalez, Javier M. Halvorson, Jonathan J. Hagerman, Ann E. TI Polyphenol-Aluminum Complex Formation: Implications for Aluminum Tolerance in Plants SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE polyphenols; pentagalloyl glucose; oenothein B; methyl gallate; aluminum detoxification; Gaussian ID ACID METHYL-ESTER; EUCALYPTUS-CAMALDULENSIS; HYDROLYZABLE TANNIN; PHENOLIC SUBSTANCES; CONDENSED TANNINS; ORGANIC-ACIDS; AL TOLERANCE; GALLIC ACID; OENOTHEIN-B; METAL-IONS AB Natural polyphenols may play an important role in aluminum detoxification in some plants. We examined the interaction between Al3+ and the purified high molecular weight polyphenols pentagalloyl glucose (940 Da) and oenothein B (1568 Da), and the related compound methyl gallate (184 Da) at pH 4 and 6. We used spectrophotometric titration and chemometric modeling to determine stability constants and stoichiometries for the aluminum phenol (AIL) complexes. The structures and spectral features of aluminum methyl gallate complexes were evaluated with quantum chemical calculations. The high molecular weight polyphenols formed Al3L2 complexes with conditional stability constants (beta) similar to 1 x 10(23) at pH 6 and AlL complexes with beta similar to 1 x 10(5) at pH 4. Methyl gallate formed AIL complexes with beta = 1 x 10(6) at pH 6 but did not complex aluminum at pH 4. At intermediate metal-to-polyphenol ratios, high molecular weight polyphenols formed insoluble Al complexes but methyl gallate complexes were soluble. The high molecular weight polyphenols have high affinities and solubility features that are favorable for a role in aluminum detoxification in the environment. C1 [Zhang, Liangliang] Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China. [Liu, Ruiqiang; Gung, Benjamin W.; Tindall, Steven; Hagerman, Ann E.] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. [Gonzalez, Javier M.] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Halvorson, Jonathan J.] USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. [Liu, Ruiqiang] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. RP Hagerman, AE (reprint author), Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. EM hagermae@miamioh.edu FU National Natural Science Foundation of China [31500485]; USDA Specific Cooperative Agreement [58-1932-6-634] FX This work was partially funded by National Natural Science Foundation of China (No. 31500485) and by USDA Specific Cooperative Agreement 58-1932-6-634 to Miami University. NR 53 TC 0 Z9 0 U1 11 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 20 PY 2016 VL 64 IS 15 BP 3025 EP 3033 DI 10.1021/acs.jafc.6b00331 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DK3JK UT WOS:000374812700004 PM 27022835 ER PT J AU Lakshman, DK Roberts, DP Garrett, WM Natarajan, SS Darwish, O Alkharouf, N Pain, A Khan, F Jambhulkar, PP Mitra, A AF Lakshman, Dilip K. Roberts, Daniel P. Garrett, Wesley M. Natarajan, Savithiry S. Darwish, Omar Alkharouf, Nadim Pain, Arnab Khan, Farooq Jambhulkar, Prashant P. Mitra, Amitava TI Proteomic Investigation of Rhizoctonia solani AG 4 Identifies Secretome and Mycelial Proteins with Roles in Plant Cell Wall Degradation and Virulence SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Rhizoctonia solani AG 4; 2-D proteomics; secretome; virulence ID STATISTICAL-MODEL; GENE-EXPRESSION; USTILAGO-MAYDIS; PATHOGENICITY; SYNTHASE; FUNGI; MAIZE; DISCOVERY; DATABASE; BLIGHT AB Rhizoctonia solani AG 4 is a soilborne necrotrophic fungal plant pathogen that causes economically important diseases on agronomic crops worldwide. This study used a proteomics approach to characterize both intracellular proteins and the secretome of R solani AG 4 isolate Rs23A under several growth conditions, the secretome being highly important in pathogenesis. From over 500 total secretome and soluble intracellular protein spots from 2-D gels, 457 protein spots were analyzed and 318 proteins positively matched with fungal proteins of known function by comparison with available R solani genome databases specific for anastomosis groups 1-IA, 1-IB, and 3. These proteins were categorized to possible cellular locations and functional groups and for some proteins their putative roles in plant cell wall degradation and virulence. The majority of the secreted proteins were grouped to extracellular regions and contain hydrolase activity. C1 [Lakshman, Dilip K.; Roberts, Daniel P.; Garrett, Wesley M.; Natarajan, Savithiry S.; Khan, Farooq] ARS, USDA, Beltsville, MD 20705 USA. [Darwish, Omar; Alkharouf, Nadim] Towson Univ, Comp & Informat Sci, Towson, MD 21252 USA. [Pain, Arnab] KAUST, Pathogen Genom, Thuwal 23955, Saudi Arabia. [Jambhulkar, Prashant P.] MPUA&T, Dept Plant Pathol, Udaipur 327001, India. [Mitra, Amitava] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Lakshman, DK (reprint author), ARS, USDA, Beltsville, MD 20705 USA. EM Dilip.lakshman@ars.usda.gov RI Pain, Arnab/L-5766-2015 OI Pain, Arnab/0000-0002-1755-2819 FU ARS Project [1230-22000-049-00D]; King Abdullah University of Science and Technology (KAUST) FX Funding for this research was provided by ARS Project 1230-22000-049-00D. Arnab Pain is supported by a faculty baseline funding from the King Abdullah University of Science and Technology (KAUST). NR 46 TC 1 Z9 1 U1 5 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 20 PY 2016 VL 64 IS 15 BP 3101 EP 3110 DI 10.1021/acs.jafc.5b05735 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DK3JK UT WOS:000374812700013 PM 27019116 ER PT J AU Cruz, JL Alves, AAC LeCain, DR Ellis, DD Morgan, JA AF Cruz, Jailson L. Alves, Alfredo A. C. LeCain, Daniel R. Ellis, David D. Morgan, Jack A. TI Interactive effects between nitrogen fertilization and elevated CO2 on growth and gas exchange of papaya seedlings SO SCIENTIA HORTICULTURAE LA English DT Article DE Gas exchange; Dry weight; Nitrogen-use efficiency; Leaf N concentration; Climate change ID CASSAVA MANIHOT-ESCULENTA; ATMOSPHERIC CO2; PLANT-GROWTH; ENRICHMENT FACE; CARBON-DIOXIDE; PHOTOSYNTHETIC CAPACITY; SEASONAL-CHANGES; WATER RELATIONS; LEAF GROWTH; RESPONSES AB Elevated atmospheric CO2 will change the requirements plants have for minerals, mainly nitrogen, altering the relationship between nutrient demand and plant growth. Here, we evaluated the interacting effects between two concentrations of CO2 (390 or 750 mu LL-1) and two nitrogen (N) levels (3 mM or 8 mM) on gas exchange, N use efficiency (NUE), and dry mass responses of papaya seedlings. The study was conducted in a climate-controlled greenhouse using 3.5 L pots and six replicates for 62 days. No significant effects from N or CO2 were noticed for leaf conductance to water vapor and transpiration. However, CO2 elevation to 750 mu LL-1 increased assimilation rate for both N levels. Elevated CO2 resulted in an increase of 52% and 16% of instantaneous water-use efficiency under high and low N, respectively, as compared to ambient CO2 (390 mu LL-1). Lower N concentrations were observed for all the organs for plants grown under higher concentrations of CO2, regardless of the N level used. However, plants grown under elevated CO2 reached the highest NUEs. There was an N x CO2 interactive effect on the leaf, stem plus petiole, and root dry mass. However, no significant CO2 effect on the root:shoot ratio was observed. Our results revealed that elevated CO2 stimulated total dry mass accumulation of papaya plants, with the results being greatest for plants grown under lower levels of N. Published by Elsevier B.V. C1 [Cruz, Jailson L.] Embrapa Mandioca & Fruticultura, CP 007, BR-44380000 Cruz Das Almas, BA, Brazil. [Cruz, Jailson L.; LeCain, Daniel R.; Morgan, Jack A.] ARS, Crop Res Lab, USDA, Ft Collins, CO 80526 USA. [Alves, Alfredo A. C.] ARS, Embrapa Labex Program, USDA, Ft Collins, CO 80521 USA. [Alves, Alfredo A. C.; Ellis, David D.] ARS, Natl Ctr Genet Resources Preservat, USDA, Ft Collins, CO 80521 USA. [Cruz, Jailson L.; Alves, Alfredo A. C.] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. [Ellis, David D.] Int Potato Ctr, Lima, Peru. RP Cruz, JL (reprint author), Embrapa Mandioca & Fruticultura, CP 007, BR-44380000 Cruz Das Almas, BA, Brazil.; Cruz, JL (reprint author), ARS, Crop Res Lab, USDA, Ft Collins, CO 80526 USA.; Cruz, JL (reprint author), Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. EM jailson.cruz@embrapa.br FU United States Department of Agriculture/USDA-ARS; Brazilian Agricultural Research Corporation FX This research was partially supported by United States Department of Agriculture/USDA-ARS and Brazilian Agricultural Research Corporation. The authors gratefully acknowledge Deborah Pelacani Cruz and Maria Claudia P. Cruz for valuable help in growing plants. NR 61 TC 0 Z9 0 U1 13 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD APR 20 PY 2016 VL 202 BP 32 EP 40 DI 10.1016/j.scienta.2016.02.010 PG 9 WC Horticulture SC Agriculture GA DK0OB UT WOS:000374611000005 ER PT J AU Overturf, K Barrows, FT Hardy, R Brezas, A Dumas, A AF Overturf, Ken Barrows, Frederic T. Hardy, RonaldW. Brezas, Andreas Dumas, Andre TI Energy composition of diet affects muscle fiber recruitment, body composition, and growth trajectory in rainbow trout (Oncorhynchus mykiss) SO AQUACULTURE LA English DT Article DE Fish nutrition; Gene expression; Modeling; Muscle growth; Nutrient deposition; Nutrigenomics ID SALMON SALMO-SALAR; ATLANTIC SALMON; SKELETAL-MUSCLE; FEED-UTILIZATION; GENE-EXPRESSION; PROTEIN-TURNOVER; LIPID-LEVELS; PROXIMATE COMPOSITION; NUTRIENT DEPOSITION; FLESH QUALITY AB Increasing feed efficiency and muscle growth in aquaculture are of high priority and require understanding how dietary components are interactively processed and trigger molecular, tissue and whole-body responses. A 67-day trial was conducted to describe the effects of three feeds with varying dietary protein (P) to lipid (L) ratios (43P:20L, 50P:15L, 62P:6L) on growth trajectory, body composition, nutrient deposition, muscle histology, gene expression and protein degradation pathways of juvenile rainbow trout (initial weight 11.2 g). The growth trajectories of trout fed the 43P:20L and 50P:15L diets were identical and higher than trout fed the 62P:6L diet. The overall TGC was 0.261 for trout fed the 43P:20L and 50P:15L diets. Feed intake was associated with dietary protein level, not energy. Body composition differed among treatments early (at day 14) in the experiment and continued throughout. Body lipid and body protein contents varied significantly (P < 0.05) from 7.36 (62P:6L) to 12.91% (43P:20L) and 14.95 (43P:20L) to 16.24% (50P:15L) at day 67, respectively). Simple mathematical models were developed to predict body lipid content and feed intake of growing trout. Analysis of gene expression data using RT-PCR correlated with other measured parameters, provided significant information to evaluate metabolic processing of the different diets. The relative expression level of Pax7 in fish fed the 43P:20L diet was higher than in fish fed the other diets and the statistical difference was nearly significant (P=0.058). The relative content of small muscle fibers (0-25 mu m) increased significantly (P < 0.05) with time in trout fed the 43P:20L diet, whereas the opposite was observed in fish fed the other treatments. An increased proportion of small muscle fibers was associated with the highest relative expression of PAX7 over time and increased lysosomal activity in fish fed the 43P:20L dietary treatment. This is the first study in vivo using fish to show that increases in hyperplasia is linked to autophagy activity. The overall rate of protein deposition was significantly (P < 0.05) higher in trout fed the 50P:15L (16.58 +/- 0.57 mg (degrees C-d)(-1)) compared to the 62P:6L (13.26 +/- 0.66 mg (degrees C-d)(-1)), but there was no significant difference with trout fed the 43P:20L diet (15.05 +/- 0.69 mg (degrees C-d)(-1)). This study demonstrates that macronutrients are potent regulators of muscle development and growth, and provides new opportunities in nutrigenomics to program performance and flesh quality of organisms. Statement of relevance:Nutrigenomics can foster sustainable aquaculture. (C) 2016 Elsevier B.V. All rights reserved. C1 [Overturf, Ken; Barrows, Frederic T.] ARS, USDA, Hagerman Fish Culture Expt Stn, 3059-F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. [Hardy, RonaldW.; Brezas, Andreas] Univ Idaho, Inst Aquaculture Res, 3059-F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. [Dumas, Andre] Coastal Zones Res Inst, 232B Ave Eglise, Shippegan, NB E8S 1J2, Canada. RP Dumas, A (reprint author), Ctr Aquaculture Technol Canada, 20 Hope St,POB 388, Souris, PE C0A 2B0, Canada. EM adumas@aquatechcenter.com FU Canadian Department of Foreign Affairs through the International Trade's North American Platform Program; Talent Recruitment Funding Program of the New Brunswick Innovation Foundation; USDA-Agricultural Research Service (CRIS project) [5366-21310-004-00D] FX Financial support of this study was provided by the Canadian Department of Foreign Affairs through the International Trade's North American Platform Program. Andre Dumas is the recipient of the Talent Recruitment Funding Program of the New Brunswick Innovation Foundation. This work was in part funded by the USDA-Agricultural Research Service (CRIS project 5366-21310-004-00D) to KO. The mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. NR 104 TC 1 Z9 1 U1 10 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD APR 20 PY 2016 VL 457 BP 1 EP 14 DI 10.1016/j.aquaculture.2016.02.002 PG 14 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA DG0ZC UT WOS:000371793800001 ER PT J AU Chen, MH Bowman, MJ Cotta, MA Dien, BS Iten, LB Whitehead, TR Rausch, KD Tumbleson, ME Singh, V AF Chen, Ming-Hsu Bowman, Michael J. Cotta, Michael A. Dien, Bruce S. Iten, Loren B. Whitehead, Terence R. Rausch, Kent D. Tumbleson, M. E. Singh, Vijay TI Miscanthus x giganteus xylooligosaccharides: Purification and fermentation SO CARBOHYDRATE POLYMERS LA English DT Article DE Miscanthus x giganteus; Autohydrolysis; Xylooligosaccharides; Fermentation; Acetic acid; Lactic acid ID IN-VITRO FERMENTATION; XYLO-OLIGOSACCHARIDES; ENZYMATIC-HYDROLYSIS; STRUCTURAL-CHARACTERIZATION; RUMINAL BACTERIA; AUTOHYDROLYSIS; PRETREATMENT; XOS; FERMENTABILITY; BIFIDOBACTERIA AB A procedure was developed to recover xylooligosaccharides (XOS) from Miscanthus x giganteus (M x G) hydrolyzate. M x G hydrolyzate was prepared using autohydrolysis, and XOS rich fractions were acquired using activated carbon adsorption and stepwise ethanol elution. The combined XOS fractions were purified using a series of ion exchange resin treatments. The end product, M x G XOS, had 89.1% (w/w) total substituted oligosaccharides (TSOS) composed of arabinose, glucose, xylose and acetyl group. Bifidobacterium adolescentis and Bifidobacterium catenulatum (health promoting bacteria) were cultured in vitro on M x G XOS and a commercial XOS source, which was used as a comparison. B. adolescentis grew to a higher cell density than B. catenulatum in both XOS cultures. Total xylose consumption for B. adolescentis was 84.1 and 84.8%, respectively for M x G and commercial XOS cultures; and for B. catenulatum was 76.6 and 73.6%, respectively. The xylobiose (X2), xylotriose (X3) and xylotetraose (X4) were almost utilized for both strains. Acetic and lactic acids were the major fermentation products of the XOS cultures. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chen, Ming-Hsu; Rausch, Kent D.; Tumbleson, M. E.; Singh, Vijay] Univ Illinois, Dept Agr & Biol Engn, 1304 W Penn Ave, Urbana, IL 61801 USA. [Bowman, Michael J.; Cotta, Michael A.; Dien, Bruce S.; Iten, Loren B.; Whitehead, Terence R.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. RP Singh, V (reprint author), Univ Illinois, Dept Agr & Biol Engn, 1304 W Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011 68005-30411] FX We thank Victoria Nguyen and Ronda Zeltwanger for their fine technical work. Victoria Nguyen is supported by the Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [grant number 2011 68005-30411]. We also thank Wei Liu for her help on HPLC analysis. NR 37 TC 0 Z9 0 U1 9 U2 27 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD APR 20 PY 2016 VL 140 BP 96 EP 103 DI 10.1016/j.carbpol.2015.12.052 PG 8 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DD5JA UT WOS:000369958300013 PM 26876832 ER PT J AU Xu, JY Krietemeyer, EF Finkenstadt, VL Solaiman, D Ashby, RD Garcia, RA AF Xu, Jingyuan Krietemeyer, Elizabeth F. Finkenstadt, Victoria L. Solaiman, Daniel Ashby, Richard D. Garcia, Rafael A. TI Preparation of starch-poly-glutamic acid graft copolymers by microwave irradiation and the characterization of their properties SO CARBOHYDRATE POLYMERS LA English DT Article DE Graft copolymers; Poly-glutamic acid (PGA); Starch AB Graft copolymers of waxy maize starch and poly y-glutamic acid (PGA) were produced in an aqueous solution using microwave irradiation. The microwave reaction conditions were optimized with regard to temperature and pH. The temperature of 180 degrees C and pH7.0 were the best reaction conditions resulting in a PGA graft of 0.45% based on nitrogen analysis. The average graft content and graft efficiency for the starch-PGA graft copolymer prepared at 180 degrees C and pH7.0 were 4.20% and 2.73%, respectively. The starch-PGA graft copolymer produced at 180 degrees C and pH7.0 could absorb more than 20 times its own weight amount of water and form a gel. The preliminary rheology study revealed that the starch-PGA graft copolymer gel exhibited viscoelastic solid behavior while the control sample of waxy starch showed viscoelastic liquid behavior. Published by Elsevier Ltd. C1 [Xu, Jingyuan; Krietemeyer, Elizabeth F.; Finkenstadt, Victoria L.] ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Solaiman, Daniel; Ashby, Richard D.; Garcia, Rafael A.] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Xu, JY (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM james.xu@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service FX This work was financially supported by US Department of Agriculture, Agricultural Research Service. NR 22 TC 1 Z9 1 U1 8 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD APR 20 PY 2016 VL 140 BP 233 EP 237 DI 10.1016/j.carbpol.2015.12.034 PG 5 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DD5JA UT WOS:000369958300030 PM 26876849 ER PT J AU Kenar, JA Compton, DL Little, JA Peterson, SC AF Kenar, James A. Compton, David L. Little, Jeanette A. Peterson, Steve C. TI Formation of inclusion complexes between high amylose starch and octadecyl ferulate via steam jet cooking SO CARBOHYDRATE POLYMERS LA English DT Article DE Ferulic acid; Lipophilization; Octadecyl ferulate; Amylose inclusion complex; Steam jet cooking; High amylose starch ID X-RAY-DIFFRACTION; FATTY-ACID COMPLEXES; CRYSTAL TRANSFORMATIONS; MOLECULAR ENCAPSULATION; ORGANIC COMPOUNDS; CHLOROGENIC ACID; COOKED MIXTURES; SMALL LIGANDS; BEHAVIOR; SPHERULITES AB Amylose-ligand inclusion complexes represent an interesting approach to deliver bioactive molecules. However, ferulic acid has been shown not to form single helical inclusion complexes with amylose from high amylose maize starch. To overcome this problem a lipophilic ferulic acid ester, octadecyl ferulate, was prepared and complexed with amylose via excess steam jet cooking. Jet-cooking octadecyl ferulate and high amylose starch gave an amylose-octadecyl ferulate inclusion complex in 51.0% isolated yield. X-ray diffraction (XRD) and differential scanning calorimetry (DSC) confirmed that a 61 V-type inclusion complex was formed. Amylose and extraction assays showed the complex to be enriched in amylose (91.9 + 4.3%) and contain 70.6 + 5.6 mgg(-1) octadecyl ferulate, although, minor hydrolysis (-4%) of the octadecyl ferulate was observed under the excess steam jet-cooking conditions utilized. This study demonstrates that steam jet cooking is a rapid and scalable process in which to prepare amylose-octadecyl ferulate inclusion complexes. Published by Elsevier Ltd. C1 [Kenar, James A.; Little, Jeanette A.] ARS, Funct Food Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815N,Univ St, Peoria, IL 61604 USA. [Compton, David L.] ARS, Renewable Product Technol Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815N,Univ St, Peoria, IL 61604 USA. [Peterson, Steve C.] ARS, Plant Polymer Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815N,Univ St, Peoria, IL 61604 USA. RP Kenar, JA (reprint author), ARS, Funct Food Res Unit, USDA, Natl Ctr Agr Utilizat Res, 1815N,Univ St, Peoria, IL 61604 USA. EM Jim.Kenar@ars.usda.gov NR 44 TC 0 Z9 0 U1 6 U2 23 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD APR 20 PY 2016 VL 140 BP 246 EP 252 DI 10.1016/j.carbpol.2015.12.048 PG 7 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DD5JA UT WOS:000369958300032 PM 26876851 ER PT J AU Hempel, RJ Bannantine, JP Stabel, JR AF Hempel, Randy J. Bannantine, John P. Stabel, Judith R. TI Transcriptional Profiling of Ileocecal Valve of Holstein Dairy Cows Infected with Mycobacterium avium subsp Paratuberculosis SO PLOS ONE LA English DT Article ID BLOOD MONONUCLEAR-CELLS; LARGE GENE LISTS; DIFFERENTIAL EXPRESSION; BOVINE PARATUBERCULOSIS; SSP PARATUBERCULOSIS; MACROPHAGE RESPONSE; IMMUNE-RESPONSES; IN-VITRO; T-CELLS; TUBERCULOSIS AB Johne's disease is a chronic infection of the small intestine caused by Mycobacterium avium subspecies paratuberculosis (MAP), an intracellular bacterium. The events of pathogen survival within the host cell(s), chronic inflammation and the progression from asymptomatic subclinical stage to an advanced clinical stage of infection, are poorly understood. This study examines gene expression in the ileocecal valve (ICV) of Holstein dairy cows at different stages of MAP infection. The ICV is known to be a primary site of MAP colonization and provides an ideal location to identify genes that are relevant to the progression of this disease. RNA was prepared from ICV tissues and RNA-Seq was used to compare gene transcription between clinical, subclinical, and uninfected control animals. Interpretation of the gene expression data was performed using pathway analysis and gene ontology categories containing multiple differentially expressed genes. Results demonstrated that many of the pathways that had strong differential gene expression between uninfected control and clinical cows were related to the immune system, such as the T-and B-cell receptor signaling, apoptosis, NOD-like receptor signaling, and leukocyte transendothelial migration pathways. In contrast, the comparison of gene transcription between control and subclinical cows identified pathways that were primarily involved in metabolism. The results from the comparison between clinical and subclinical animals indicate recruitment of neutrophils, up regulation of lysosomal peptidases, increase in immune cell transendothelial migration, and modifications of the extracelluar matrix. This study provides important insight into how cattle respond to a natural MAP infection at the gene transcription level within a key target tissue for infection. C1 [Hempel, Randy J.; Bannantine, John P.; Stabel, Judith R.] USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. RP Stabel, JR (reprint author), USDA ARS, Natl Anim Dis Ctr, POB 70, Ames, IA 50010 USA. EM judy.stabel@ars.usda.gov OI Bannantine, John/0000-0002-5692-7898 NR 60 TC 0 Z9 0 U1 1 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 19 PY 2016 VL 11 IS 4 AR e0153932 DI 10.1371/journal.pone.0153932 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ9NW UT WOS:000374541200064 PM 27093613 ER PT J AU Wilkinson, JM Ladinig, A Bao, H Kommadath, A Stothard, P Lunney, JK Harding, JCS Plastow, GS AF Wilkinson, Jamie M. Ladinig, Andrea Bao, Hua Kommadath, Arun Stothard, Paul Lunney, Joan K. Harding, John C. S. Plastow, Graham S. TI Differences in Whole Blood Gene Expression Associated with Infection Time-Course and Extent of Fetal Mortality in a Reproductive Model of Type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection SO PLOS ONE LA English DT Article ID IMMUNE-RESPONSES; PREGNANT GILTS; VIRAL LOAD; RNA-SEQ; ACTIVATION; PLATELETS; PIGS; APOPTOSIS; PROTEINS; IDENTIFICATION AB Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant females causes fetal death and increased piglet mortality, but there is substantial variation in the extent of reproductive pathology between individual dams. This study used RNA sequencing to characterize the whole blood transcriptional response to type 2 PRRSV in pregnant gilts during the first week of infection (at 0, 2, and 6 days post-inoculation), and attempted to identify gene expression signatures associated with a low or high level of fetal mortality rates (LFM and HFM; n = 8/group) at necropsy, 21 days post-inoculation. The initial response to infection measured at 2 days post -inoculation saw an upregulation of genes involved in innate immunity, such as interferon-stimulated antiviral genes and inflammatory markers, and apoptosis. A concomitant decrease in expression of protein synthesis and T lymphocyte markers was observed. By day 6 the pattern had reversed, with a drop in innate immune signaling and an increase in the expression of genes involved in cell division and T cell signaling. Differentially expressed genes (DEGs) associated with extremes of litter mortality rate were identified at all three time -points. Among the 15 DEGs upregulated in LFM gilts on all three days were several genes involved in platelet function, including integrins ITGA2B and ITGB3, and the chemokine PF4 (CXCL4). LFM gilts exhibited a higher baseline expression of interferon-stimulated and pro -inflammatory genes prior to infection, and of T cell markers two days post-infection, indicative of a more rapid progression of the immune response to PRRSV. This study has increased our knowledge of the early response to PRRSV in the blood of pregnant gilts, and could ultimately lead to the development of a biomarker panel that can be used to predict PRRSV-associated reproductive pathology. C1 [Wilkinson, Jamie M.; Bao, Hua; Kommadath, Arun; Stothard, Paul; Plastow, Graham S.] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. [Ladinig, Andrea; Harding, John C. S.] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. [Lunney, Joan K.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. [Ladinig, Andrea] Univ Vet Med, Dept Farm Anim & Vet Publ Hlth, Univ Clin Swine, Vienna, Austria. RP Wilkinson, JM (reprint author), Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. EM jamie.wilkinson@ualberta.ca OI Ladinig, Andrea/0000-0001-5037-7269 FU Genome Canada; Genome Prairie; Alberta Livestock and Meat Agency; Alberta Innovates - Bio Solutions FX This work was funded by grants from Genome Canada (genomecanada.ca) and Genome Prairie (genomeprairie.ca) to support the following project: "Application of Genomics to Improve Swine Health and Welfare". Administrative support was provided by Genome Alberta (genomealberta.ca). HB and PS are grateful for financial support from the Alberta Livestock and Meat Agency (alma.alberta.ca) and Alberta Innovates - Bio Solutions (bio.albertainnovates.ca), respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 0 Z9 0 U1 4 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 19 PY 2016 VL 11 IS 4 AR e0153615 DI 10.1371/journal.pone.0153615 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ9NW UT WOS:000374541200032 PM 27093427 ER PT J AU Parra-Flores, J Juneja, V de Fernando, GG Aguirre, J AF Parra-Flores, Julio Juneja, Vijay Garcia de Fernando, Gonzalo Aguirre, Juan TI Variability in Cell Response of Cronobacter sakazakii after Mild-Heat Treatments and Its Impact on Food Safety SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Cronobacter sakazakii; PIF/FUF; food safety; variability; risk assessment; heat treatment ID POWDERED INFANT FORMULA; LAG TIME DISTRIBUTIONS; ENTEROBACTER-SAKAZAKII; LISTERIA-MONOCYTOGENES; ESCHERICHIA-COLI; SINGLE CELLS; SENSITIVITY-ANALYSIS; INDIVIDUAL CELLS; NECROTIZING ENTEROCOLITIS; PROCESSING ENVIRONMENT AB Cronobacter spp. have been responsible for severe infections in infants associated with consumption of powdered infant formula and follow-up formulae. Despite several risk assessments described in published studies, few approaches have considered the tremendous variability in cell response that small micropopulations or single cells can have in infant formula during storage, preparation or post process/preparation before the feeding of infants. Stochastic approaches can better describe microbial single cell response than deterministic models as we prove in this study. A large variability of lag phase was observed in single cell and micropopulations of <= 50 cells. This variability increased as the heat shock increased and growth temperature decreased. Obviously, variability of growth of individual Cronobacter sakazakii cell is affected by inoculum size, growth temperature and the probability of cells able to grow at the conditions imposed by the experimental conditions should be taken into account, especially when errors in bottle preparation practices, such as improper holding temperatures, or manipulation, may lead to growth of the pathogen to a critical cell level. The mean probability of illness from initial inoculum size of 1 cell was below 0.2 in all the cases and for inoculum size of 50 cells the mean probability of illness, in most of the cases, was above 0.7. C1 [Parra-Flores, Julio] Univ Bio Bio, Dept Nutr & Salud Publ, Chillan, Chile. [Juneja, Vijay] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA. [Garcia de Fernando, Gonzalo; Aguirre, Juan] Univ Chile, Inst Nutr & Tecnol Alimentos, Lab Microbiol & Probiot, Santiago, Chile. [Aguirre, Juan] Univ Complutense, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain. RP Aguirre, J (reprint author), Univ Chile, Inst Nutr & Tecnol Alimentos, Lab Microbiol & Probiot, Santiago, Chile.; Aguirre, J (reprint author), Univ Complutense, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain. EM juaguirr@vet.ucm.es RI Aguirre Garcia, Juan/B-2777-2015 FU DIUBB Chile [143720]; Program Consolider CARNISENUSA Spain [CSD2007-0016] FX This work was partially supported by DIUBB Chile, project 143720 and the Program Consolider CARNISENUSA Spain CSD2007-0016. NR 101 TC 0 Z9 0 U1 8 U2 19 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 19 PY 2016 VL 7 AR 535 DI 10.3389/fmicb.2016.00535 PG 14 WC Microbiology SC Microbiology GA DJ5WT UT WOS:000374282200001 PM 27148223 ER PT J AU Yan, Q Philmus, B Hesse, C Kohen, M Chang, JH Loper, JE AF Yan, Qing Philmus, Benjamin Hesse, Cedar Kohen, Max Chang, Jeff H. Loper, Joyce E. TI The Rare Codon AGA Is Involved in Regulation of Pyoluteorin Biosynthesis in Pseudomonas protegens Pf-5 SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Pseudomonas protegens; rare codon; AGA codon; pyoluteorin; regulation ID ANTIFUNGAL METABOLITE PRODUCTION; STREPTOMYCES-COELICOLOR A3(2); COMPLETE GENOME SEQUENCE; ARGININE TRANSFER-RNA; FLUORESCENS PF-5; BIOLOGICAL-CONTROL; ESCHERICHIA-COLI; COTTON SEEDLINGS; GENE-EXPRESSION; DAMPING-OFF AB The soil bacterium Pseudomonas protegens Pf-5 can colonize root and seed surfaces of many plants, protecting them from infection by plant pathogenic fungi and oomycetes. The capacity to suppress disease is attributed to Pf-5's production of a large spectrum of antibiotics, which is controlled by complex regulatory circuits operating at the transcriptional and post-transcriptional levels. In this study, we analyzed the genomic sequence of Pf-5 for codon usage patterns and observed that the six rarest codons in the genome are present in all seven known antibiotic biosynthesis gene clusters. In particular, there is an abundance of rare codons in OR, which encodes a member of the LysR transcriptional regulator family that controls the expression of pyoluteorin biosynthetic genes. To test the hypothesis that rare codons in pltR influence pyoluteorin production, we generated a derivative of Pf-5 in which 23 types of rare codons in pltR were substituted with synonymous preferred codons. The resultant mutant produced pyoluteorin at levels 15 times higher than that of the wild-type Pf-5. Accordingly, the promoter activity of the pyoluteorin biosynthetic gene pltL was 20 times higher in the codon-modified stain than in the wild-type. pltR has six AGA codons, which is the rarest codon in the Pf-5 genome. Substitution of all six AGA codons with preferred Arg codons resulted in a variant of OR that conferred increased pyoluteorin production and pltL promoter activity. Furthermore, overexpression of tRNA(UCU)(Arg),, the cognate tRNA for the AGA codon, significantly increased pyoluteorin production by Pf-5. A bias in codon usage has been linked to the regulation of many phenotypes in eukaryotes and prokaryotes but, to our knowledge, this is the first example of the role of a rare codon in the regulation of antibiotic production by a Gram-negative bacterium. C1 [Yan, Qing; Kohen, Max; Chang, Jeff H.; Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Philmus, Benjamin] Oregon State Univ, Coll Pharm, Corvallis, OR 97331 USA. [Hesse, Cedar; Loper, Joyce E.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. RP Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Loper, JE (reprint author), ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. EM loperj@science.oregonstate.edu OI Loper, Joyce/0000-0003-3501-5969 FU Agriculture and Food Research Initiative Competitive Grants from the United States Department of Agriculture National Institute of Food and Agriculture [2011-67019-30192]; College of Pharmacy, Oregon State University FX We gratefully acknowledge the assistance of Brenda Shaffer in carrying out the experiments described herein and Jennifer Clifford for helpful insights into codon usage and tRNAs in the Pf-5 genome. This work was supported by Agriculture and Food Research Initiative Competitive Grants 2011-67019-30192 from the United States Department of Agriculture National Institute of Food and Agriculture, and funds from the College of Pharmacy, Oregon State University. NR 53 TC 0 Z9 0 U1 3 U2 6 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 19 PY 2016 VL 7 AR 497 DI 10.3389/fmicb.2016.00497 PG 14 WC Microbiology SC Microbiology GA DJ5WJ UT WOS:000374281200002 PM 27148187 ER PT J AU Isaak, DJ Young, MK Luce, CH Hostetler, SW Wenger, SJ Peterson, EE Hoef, JMV Groce, MC Horan, DL Nagel, DE AF Isaak, Daniel J. Young, Michael K. Luce, Charles H. Hostetler, Steven W. Wenger, Seth J. Peterson, Erin E. Hoef, Jay M. Ver Groce, Matthew C. Horan, Dona L. Nagel, David E. TI Slow climate velocities of mountain streams portend their role as refugia for cold-water biodiversity SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE climate refugia; climate velocity; biodiversity; fish; network ID CUTTHROAT TROUT; THERMAL SENSITIVITY; AIR-TEMPERATURE; BROOK TROUT; NETWORKS; HABITAT; FISHES; POPULATIONS; PERSISTENCE; TELEOSTEI AB The imminent demise of montane species is a recurrent theme in the climate change literature, particularly for aquatic species that are constrained to networks and elevational rather than latitudinal retreat as temperatures increase. Predictions of widespread species losses, however, have yet to be fulfilled despite decades of climate change, suggesting that trends are much weaker than anticipated and may be too subtle for detection given the widespread use of sparse water temperature datasets or imprecise surrogates like elevation and air temperature. Through application of large water-temperature databases evaluated for sensitivity to historical air-temperature variability and computationally interpolated to provide high-resolution thermal habitat information for a 222,000-km network, we estimate a less dire thermal plight for cold-water species within mountains of the northwestern United States. Stream warming rates and climate velocities were both relatively low for 1968-2011 (average warming rate = 0.101 degrees C/ decade; median velocity = 1.07 km/decade) when air temperatures warmed at 0.21 degrees C/decade. Many cold-water vertebrate species occurred in a subset of the network characterized by low climate velocities, and three native species of conservation concern occurred in extremely cold, slow velocity environments (0.33-0.48 km/decade). Examination of aggressive warming scenarios indicated that although network climate velocities could increase, they remain low in headwaters because of strong local temperature gradients associated with topographic controls. Better information about changing hydrology and disturbance regimes is needed to complement these results, but rather than being climatic cul-de-sacs, many mountain streams appear poised to be redoubts for cold-water biodiversity this century. C1 [Isaak, Daniel J.; Luce, Charles H.; Groce, Matthew C.; Horan, Dona L.; Nagel, David E.] US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. [Young, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Hostetler, Steven W.] US Geol Survey, Corvallis, OR 97331 USA. [Wenger, Seth J.] Univ Georgia, River Basins Ctr, Athens, GA 30602 USA. [Peterson, Erin E.] Queensland Univ Technol, Australian Res Council, Ctr Excellence Math & Stat Frontiers, Brisbane, Qld 4000, Australia. [Peterson, Erin E.] Queensland Univ Technol, Inst Future Environm, Brisbane, Qld 4000, Australia. [Hoef, Jay M. Ver] NOAA, Natl Marine Fisheries Serv, Alaska Fisheries Sci Ctr, Natl Marine Mammal Lab, Seattle, WA 98112 USA. RP Isaak, DJ (reprint author), US Forest Serv, Rocky Mt Res Stn, Boise, ID 83702 USA. EM disaak@fs.fed.us RI Luce, Charles/A-9267-2008; OI Luce, Charles/0000-0002-6938-9662; Peterson, Erin/0000-0003-2992-0372; Ver Hoef, Jay/0000-0003-4302-6895 FU US Forest Service Rocky Mountain Research Station; US Fish and Wildlife Service's Great Northern; North Pacific Landscape Conservation Cooperatives FX This research was supported by the US Forest Service Rocky Mountain Research Station and grants from the US Fish and Wildlife Service's Great Northern and North Pacific Landscape Conservation Cooperatives. NR 54 TC 6 Z9 6 U1 13 U2 22 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 19 PY 2016 VL 113 IS 16 BP 4374 EP 4379 DI 10.1073/pnas.1522429113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ7LV UT WOS:000374393800048 PM 27044091 ER PT J AU Ito, J Nakagawa, K Kato, S Miyazawa, T Kimura, F Miyazawa, T AF Ito, Junya Nakagawa, Kiyotaka Kato, Shunji Miyazawa, Taiki Kimura, Fumiko Miyazawa, Teruo TI The combination of maternal and offspring high-fat diets causes marked oxidative stress and development of metabolic syndrome in mouse offspring SO LIFE SCIENCES LA English DT Article DE PCOOH; Oxidative stress; Fetal programming model; Gpx4; Metabolic syndrome ID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE; LIPID-PEROXIDATION; PHOSPHATIDYLCHOLINE HYDROPEROXIDE; LIQUID-CHROMATOGRAPHY; GENE-EXPRESSION; HUMAN PLASMA; ACID; MICE; RATS; AGE AB Maternal overnutrition (e.g., high-fat (HF) diet) during pregnancy and lactation is believed to cause oxidative stress and increase the risk of metabolic syndrome in offspring. In the present study, we investigated the effects of both maternal and offspring HF diets on metabolic syndrome risk and oxidative stress profiles in mice. Dams of the C57BL/6J mouse strain were fed a HF or control (CO) diet during gestation and lactation. Offspring were weaned at 3 weeks of age. The female offspring were sacrificed at weaning, while the males were maintained on the HF or CO diet until 11 weeks of age. Tissue samples, including those from liver, were collected from offspring at 3 and 11 weeks of age, and lipids, phosphatidylcholine hydroperoxide (PCOOH, an oxidative stress marker), and gene expression were evaluated. Accumulation of lipids, but not PCOOH, was found in the livers of 3-week-old offspring from dams fed the HF diet. When the offspring were maintained on a HF diet until 11 weeks of age, marked accumulation of both liver lipids and PCOOH was observed. PCOOH manifestation was supported by the expression of genes such as Gpx4, encoding a PCOOH degrading enzyme. These results suggest that the combination of maternal and offspring overnutrition causes marked oxidative stress in offspring, which accelerates metabolic syndrome. The present findings in offspring from infancy to adulthood may be useful for better understanding the cause-and-effect relationships between oxidative stress and metabolic syndrome development. (C) 2016 Elsevier Inc. All rights reserved. C1 [Ito, Junya; Nakagawa, Kiyotaka; Kimura, Fumiko; Miyazawa, Teruo] Tohoku Univ, Grad Sch Agr Sci, Food & Biodynam Chem Lab, Sendai, Miyagi 9818555, Japan. [Kato, Shunji] Nippon Med Sch, Dept Med, Div Endocrinol & Metab, Tokyo 1138603, Japan. [Miyazawa, Taiki] Tufts Univ, Vasc Biol Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Nakagawa, K (reprint author), Tohoku Univ, Grad Sch Agr Sci, Food & Biodynam Chem Lab, Aoba Ku, 1-1 Tsutsumidori Amamiyamachi, Sendai, Miyagi 9818555, Japan. EM nkgw@m.tohoku.ac.jp RI Miyazawa, Taiki/G-5994-2014 NR 33 TC 3 Z9 3 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0024-3205 EI 1879-0631 J9 LIFE SCI JI Life Sci. PD APR 15 PY 2016 VL 151 BP 70 EP 75 DI 10.1016/j.lfs.2016.02.089 PG 6 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA DL6DK UT WOS:000375728400009 PM 26924496 ER PT J AU Finley, K Chhin, S Nzokou, P O'Brien, J AF Finley, Kaelyn Chhin, Sophan Nzokou, Pascal O'Brien, Joseph TI Use of near-infrared spectroscopy as an indicator of emerald ash borer infestation in white ash stem tissue SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Agrilus planipennis; Detection tool; Forest health; Fraxinus americana; Near-infrared spectroscopy; Wood borer ID AGRILUS-PLANIPENNIS; HYPERSPECTRAL IMAGERY; UNITED-STATES; OUTER BARK; WOOD; COLEOPTERA; FOREST; BUPRESTIDAE; DECLINE; PHLOEM AB Emerald ash borer (EAB) (Agrilus planipennis), is a non-native beetle responsible for widespread mortality of several North American ash species (Fraxinus sp.). Current non-destructive methods for early detection of EAB in specific trees are limited, which restricts the effectiveness of management efforts to contain this invasive threat. This study explored the application of near-infrared (NIR) spectroscopy as a novel method for detection of EAB in white ash (Fraxinus americana). Increment borers were used to non-destructively and efficiently collect increment cores from visibly healthy and EAB infested white ash trees for subsequent NIR analysis. White ash trees were sampled across sites of different land use categories (i.e., natural forests versus recreational areas) and different latitudinal regions in the eastern Lower Peninsula and in the western Upper Peninsula of Michigan. NIR diffuse reflectance spectra were collected from three stem tissue types (outer bark, phloem, and xylem) using a NIR spectrometer over the wavelength range of 1134-2190 nm. Spectral measurements were treated with a Savitzky-Golay smoothing filter, followed by a 1st and 2nd derivative transformation to increase spectral sensitivity. Near infrared spectroscopy was successfully able to discriminate between EAB infested and visually healthy trees using step-wise linear discriminant analysis for each of the three stem tissue types (outer bark, phloem and xylem). The NIR spectra of xylem tissue transformed using a Savitzky-Golay smoothing filter with a 1st derivative transformation over the full NIR wavelength range (1134-2190 nm) had the highest classification accuracy (97%) for discriminating between healthy versus EAB infested trees. This study underscores potential for future research in developing NIR spectroscopy as a cost-effective method of early detection and monitoring of emerald ash borer and other stem-boring insects in support of current detection methods. (C) 2016 Elsevier B.V. All rights reserved. C1 [Finley, Kaelyn; Chhin, Sophan; Nzokou, Pascal] Michigan State Univ, Dept Forestry, Nat Resources Bldg,480 Wilson Rd,Room 126, E Lansing, MI 48824 USA. [O'Brien, Joseph] US Forest Serv, Forest Hlth Protect, 1992 Folwell Ave, St Paul, MN 55108 USA. RP Chhin, S (reprint author), Michigan State Univ, Dept Forestry, Nat Resources Bldg,480 Wilson Rd,Room 126, E Lansing, MI 48824 USA. EM finleyk5@msu.edu; chhin@msu.edu; nzokoupa@msu.edu; jobrien@fs.fed.us FU Michigan State University (MSU) AgBioResearch, MSU College of Agriculture and Natural Resources, MSU Department of Forestry [MICL06016]; U.S. Forest Service (Forest Health and Protection) [12-DG-11420004-208] FX We thank D. Berry, J. Brabon, B.-A. Breton, C. Johnson, R. Liborius, K. Minnix, and P. Shults for providing assistance in field sampling and sample processing. J. Angel, J. Hartman, M. Holland, R. Miller, A. Ihnken, K. Rodock, E. Thompson, P. Thompson, C. Stevens, B. VanPatten, and S. Whitcomb provided logistical support in obtaining permission to sample white ash across Michigan. Thanks also go to S. Katovich and D. McCullough for providing comments on a previous version of the manuscript. Funding for this project was provided by Michigan State University (MSU) AgBioResearch (Project # MICL06016), MSU College of Agriculture and Natural Resources, MSU Department of Forestry, and a grant from the U.S. Forest Service (Forest Health and Protection, Project # 12-DG-11420004-208). NR 72 TC 0 Z9 0 U1 15 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2016 VL 366 BP 41 EP 52 DI 10.1016/j.foreco.2016.01.032 PG 12 WC Forestry SC Forestry GA DK8EX UT WOS:000375161100005 ER PT J AU Mercader, RJ McCullough, DG Storer, AJ Bedford, JM Heyd, R Siegert, NW Katovich, S Poland, TM AF Mercader, Rodrigo J. McCullough, Deborah G. Storer, Andrew J. Bedford, John M. Heyd, Robert Siegert, Nathan W. Katovich, Steven Poland, Therese M. TI Estimating local spread of recently established emerald ash borer, Agrilus planipennis, infestations and the potential to influence it with a systemic insecticide and girdled ash trees SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Biological invasions; Buprestidae; Dispersal; Forest insect pest ID COLEOPTERA BUPRESTIDAE; EMAMECTIN BENZOATE; NORTH-AMERICAN; OUTLIER SITES; UNITED-STATES; URBAN FOREST; DISPERSAL; MANAGEMENT; POPULATIONS; INVASION AB Information on the pattern and rate of spread for invasive wood- and phloem-feeding insects, including the emerald ash borer (EAB) (Agrilus planipennis Fairmaire), is relatively limited, largely because of the difficulty of detecting subcortical insects at low densities. From 2008 to 2011, grids of girdled and subsequently debarked ash (Fraxinus spp.) detection trees were established across a >390 km(2) area encompassing two recently established EAB infestations in Michigan as part of the SLow Ash Mortality (SLAM) Pilot Project. Ash distribution and abundance were inventoried across the project area which included public and private forestland, a state park, and street trees in a small municipality. Spread rates of EAB from 2008 to 2011, based on larval presence in girdled detection trees, were estimated to be 1.2-1.7 km yr(-1) in the larger, presumably older, infestation and 0.4-0.7 km yr(-1) in the smaller infestation; suggesting a slower spread rate during the initial stages of population establishment. From 2009 to 2011, a total of 587 ash trees in the project area were trunk-injected with a highly effective, systemic emamectin benzoate insecticide. Potential effects of girdled ash trees and the systemic insecticide treatment on EAB spread were evaluated using a simulation model and a simple descriptive model of observed spread. Not surprisingly, density of trees treated with the insecticide was too low to exert a detectable effect on EAB spread. However, while the density of girdled trees was also relatively low, model results indicated a reduced spread of EAB out of areas containing girdled trees. (C) 2016 Elsevier B.V. All rights reserved. C1 [Mercader, Rodrigo J.] Washburn Univ, Dept Biol, 1700 SW Coll Ave, Topeka, KS 66621 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, 243 Nat Sci Bldg, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, 243 Nat Sci Bldg, E Lansing, MI 48824 USA. [Storer, Andrew J.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. [Bedford, John M.] Michigan Dept Agr & Rural Dev, Lansing, MI 48909 USA. [Heyd, Robert] Michigan Dept Nat Resources, 1990 US 41 South, Marquette, MI 49855 USA. [Siegert, Nathan W.] USDA, Forest Serv, Northeastern Area State & Private Forestry, Forest Hlth Protect, Durham, NH 03824 USA. [Katovich, Steven] USDA, Forest Serv, Northeastern Area State & Private Forestry, 1992 Folwell Ave, St Paul, MN 55108 USA. [Poland, Therese M.] USDA, Forest Serv, No Res Stn, 3101 Technol Blvd,Suite F, Lansing, MI 48910 USA. RP Mercader, RJ (reprint author), Washburn Univ, Dept Biol, 1700 SW Coll Ave, Topeka, KS 66621 USA. EM rodrigo.mercader@washburn.edu RI Mercader, Rodrigo/L-6550-2015 OI Mercader, Rodrigo/0000-0002-4904-2805 FU USDA Forest Service; American Recovery and Reinvestment Act; USDA APHIS FX We thank our colleagues and members of the SLAM Management Board, including Brenda Owen (MTU), Noel Schneeberger (USDA Forest Service), and Kenneth Rauscher (Michigan Dept. of Agriculture and Rural Development). We also thank Gabriel Carballo, Travis Perkins and Amos Ziegler (MSU) for their assistance with data collected for the SLAM pilot project. We appreciate the efforts of all the surveyors who collected the SLAM data, often in challenging and difficult conditions. Funding for the SLAM Pilot Project and our work was provided by the USDA Forest Service, the American Recovery and Reinvestment Act and USDA APHIS. NR 52 TC 0 Z9 0 U1 16 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2016 VL 366 BP 87 EP 97 DI 10.1016/j.foreco.2016.02.005 PG 11 WC Forestry SC Forestry GA DK8EX UT WOS:000375161100009 ER PT J AU Zald, HSJ Spies, TA Seidl, R Pabst, RJ Olsen, KA Steel, EA AF Zald, Harold S. J. Spies, Thomas A. Seidl, Rupert Pabst, Robert J. Olsen, Keith A. Steel, E. Ashley TI Complex mountain terrain and disturbance history drive variation in forest aboveground live carbon density in the western Oregon Cascades, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest carbon; Lidar; Topography; Forest management; Wildfire; Landscape heterogeneity ID NET PRIMARY PRODUCTION; DOUGLAS-FIR FORESTS; PACIFIC-NORTHWEST; CLIMATE-CHANGE; STRUCTURAL DEVELOPMENT; WINDTHROW DISTURBANCE; NORTHERN CALIFORNIA; BIOMASS ESTIMATION; DECIDUOUS FOREST; LANDSCAPE-SCALE AB Forest carbon (C) density varies tremendously across space due to the inherent heterogeneity of forest ecosystems. Variation of forest C density is especially pronounced in mountainous terrain, where environmental gradients are compressed and vary at multiple spatial scales. Additionally, the influence of environmental gradients may vary with forest age and developmental stage, an important consideration as forest landscapes often have a diversity of stand ages from past management and other disturbance agents. Quantifying forest C density and its underlying environmental determinants in mountain terrain has remained challenging because many available data sources lack the spatial grain and ecological resolution needed at both stand and landscape scales. The objective of this study was to determine if environmental factors influencing aboveground live carbon (ALC) density differed between young versus old forests. We integrated aerial light detection and ranging (lidar) data with 702 field plots to map forest ALC density at a grain of 25 m across the H.J. Andrews Experimental Forest, a 6369 ha watershed in the Cascade Mountains of Oregon, USA. We used linear regressions, random forest ensemble learning (RF) and sequential autoregressive modeling (SAR) to reveal how mapped forest ALC density was related to climate, topography, soils, and past disturbance history (timber harvesting and wildfires). ALC increased with stand age in young managed forests, with much greater variation of ALC in relation to years since wildfire in old unmanaged forests. Timber harvesting was the most important driver of ALC across the entire watershed, despite occurring on only 23% of the landscape. More variation in forest ALC density was explained in models of young managed forests than in models of old unmanaged forests. Besides stand age, ALC density in young managed forests was driven by factors influencing site productivity, whereas variation in ALC density in old unmanaged forests was also affected by finer scale topographic conditions associated with sheltered sites. Past wildfires only had a small influence on current ALC density, which may be a result of long times since fire and/or prevalence of non-stand replacing fire. Our results indicate that forest ALC density depends on a suite of multi-scale environmental drivers mediated by complex mountain topography, and that these relationships are dependent on stand age. The high and context-dependent spatial variability of forest ALC density has implications for quantifying forest carbon stores, establishing upper bounds of potential carbon sequestration, and scaling field data to landscape and regional scales. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zald, Harold S. J.; Pabst, Robert J.; Olsen, Keith A.] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Spies, Thomas A.] USDA Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Seidl, Rupert] Univ Nat Resources & Life Sci BOKU, Vienna, Austria. [Steel, E. Ashley] USDA Forest Serv, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Zald, HSJ (reprint author), Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. EM harold.zald@oregonstate.edu; tom.spies@oregon-state.edu; rupert.seidl@boku.ac.at; rob.pabst@oregonstate.edu; keith.olsen@oregonstate.edu; asteel@fs.fed.us FU H. J. Andrews Experimental Forest; National Science Foundation Long Term Ecological Research (LTER) program [NSF DEB 0823380]; Austrian Science Fund FWF [P 25503-B16]; EC Marie Curie Career Integration Grant [PCIG12-GA-2012-334104]; National Science Foundation's Long-Term Ecological Research Program [NSF DEB 0823380]; US Forest Service Pacific Northwest Research Station; Oregon State University FX Support for this research was provided by the H. J. Andrews Experimental Forest and the National Science Foundation Long Term Ecological Research (LTER) program (NSF DEB 0823380). RS acknowledges support from the Austrian Science Fund FWF (grant P 25503-B16) and an EC Marie Curie Career Integration Grant (PCIG12-GA-2012-334104). Vegetation data sets were provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (NSF DEB 0823380), US Forest Service Pacific Northwest Research Station, and Oregon State University. NR 126 TC 0 Z9 0 U1 18 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2016 VL 366 BP 193 EP 207 DI 10.1016/j.foreco.2016.01.036 PG 15 WC Forestry SC Forestry GA DK8EX UT WOS:000375161100019 PM 27041818 ER PT J AU Hessburg, PF Spies, TA Perry, DA Skinner, CN Taylor, AH Brown, PM Stephens, SL Larson, AJ Churchill, DJ Povak, NA Singleton, PH McComb, B Zielinski, WJ Collins, BM Salter, RB Keane, JJ Franklin, JF Riegel, G AF Hessburg, Paul F. Spies, Thomas A. Perry, David A. Skinner, Carl N. Taylor, Alan H. Brown, Peter M. Stephens, Scott L. Larson, Andrew J. Churchill, Derek J. Povak, Nicholas A. Singleton, Peter H. McComb, Brenda Zielinski, William J. Collins, Brandon M. Salter, R. Brion Keane, John J. Franklin, Jerry F. Riegel, Greg TI Tamm Review: Management of mixed-severity fire regime forests in Oregon, Washington, and Northern California SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Forest resilience; Resistance; Climate change; Multi-scale heterogeneity; Patch size distributions; Topographic controls; Early successional habitats ID WESTERN UNITED-STATES; LAKE TAHOE BASIN; YOSEMITE-NATIONAL-PARK; PONDEROSA PINE FOREST; FUEL-REDUCTION TREATMENTS; SOUTHERN CASCADE RANGE; OLD-GROWTH FORESTS; WILDLAND-URBAN INTERFACE; SPOTTED OWL HABITAT; STAND-DENSITY INDEX AB Increasingly, objectives for forests with moderate- or mixed-severity fire regimes are to restore successionally diverse landscapes that are resistant and resilient to current and future stressors. Maintaining native species and characteristic processes requires this successional diversity, but methods to achieve it are poorly explained in the literature. In the Inland Pacific US, large, old, early seral trees were a key historical feature of many young and old forest successional patches, especially where fires frequently occurred. Large, old trees are naturally fire-tolerant, but today are often threatened by dense understory cohorts that create fuel ladders that alter likely post-fire successional pathways. Reducing these understories can contribute to resistance by creating conditions where canopy trees will survive disturbances and climatic stressors; these survivors are important seed sources, soil protectors, and critical habitat elements. Historical timber harvesting has skewed tree size and age class distributions, created hard edges, and altered native patch sizes. Manipulating these altered forests to promote development of larger patches of older, larger, and more widely-spaced trees with diverse understories will increase landscape resistance to severe fires, and enhance wildlife habitat for underrepresented conditions. Closed-canopy, multi-layered patches that develop in hot, dry summer environments are vulnerable to droughts, and they increase landscape vulnerability to insect outbreaks and severe wildfires. These same patches provide habitat for species such as the northern spotted owl, which has benefited from increased habitat area. Regional and local planning will be critical for gauging risks, evaluating trade-offs, and restoring dynamics that can support these and other species. The goal will be to manage for heterogeneous landscapes that include variably-sized patches of (1) young, middle-aged, and old, closed canopy forests growing in upper montane, northerly aspect, and valley bottom settings, (2) a similar diversity of open-canopy, fire-tolerant patches growing on ridgetops, southerly aspects, and lower montane settings, and (3) significant montane chaparral and grassland areas. Tools to achieve this goal include managed wildfire, prescribed burning, and variable density thinning at small to large scales. Specifics on "how much and where?" will vary according to physiographic, topographic and historical templates, and regulatory requirements, and be determined by means of a socio-ecological process. Published by Elsevier B.V. C1 [Hessburg, Paul F.] USDA, Forest Serv, Pacific NW Res Stn, 1133 North Western Ave, Wenatchee, WA 98801 USA. [Spies, Thomas A.] USDA, Forest Serv, Pacific NW Res Stn, Corvallis, OR USA. [Perry, David A.; McComb, Brenda] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Skinner, Carl N.] USDA, Forest Serv, Pacific Southwest Res Stn, Redding, CA USA. [Taylor, Alan H.] Penn State Univ, Dept Geog, University Pk, PA 16802 USA. [Taylor, Alan H.] Penn State Univ, Earth & Environm Syst Inst, University Pk, PA 16802 USA. [Brown, Peter M.] Rocky Mt Tree Ring Res, 2901 Moore Lane, Ft Collins, CO 80526 USA. [Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Larson, Andrew J.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Churchill, Derek J.; Franklin, Jerry F.] Univ Washington, Coll Environm, Sch Forest Resources, Seattle, WA 98195 USA. [Povak, Nicholas A.; Singleton, Peter H.; Salter, R. Brion] USDA, Forest Serv, Pacific NW Res Stn, Wenatchee, WA USA. [Zielinski, William J.] USDA, Forest Serv, Pacific NW Res Stn, Arcata, CA USA. [Collins, Brandon M.] USDA, Forest Serv, Pacific NW Res Stn, Davis, CA USA. [Keane, John J.] USDA, Forest Serv, Pacific NW Res Stn, Fresno, CA USA. [Riegel, Greg] USDA, Forest Serv, Deschutes Natl Forest, Bend, OR USA. RP Hessburg, PF (reprint author), USDA, Forest Serv, Pacific NW Res Stn, 1133 North Western Ave, Wenatchee, WA 98801 USA. EM phessburg@fs.fed.us; tspies@fs.fed.us; dave_perry38@msn.com; rxfuego@gmail.com; aht1@psu.edu; pmb@rmtrr.org; sstephens@berkeley.edu; a.larson@umontana.edu; derekch@u.washington.edu; npovak@fs.fed.us; psingleton@fs.fed.us; Brenda.McComb@oregonstate.edu; bzielinski@fs.fed.us; bmcollins@fs.fed.us; bsalter@fs.fed.us; jkeane@fs.fed.us; jff@u.washington.edu; griegel@fs.fed.us FU Pacific Northwest Research Station; National Fire Plan; Okanogan-Wenatchee National Forest; Joint Fire Sciences Program; Pacific Southwest Research Station FX We gratefully acknowledge the helpful reviews of Bill Gaines, James Dickinson, Keala Hagmann, Rick Brown, Richy Harrod, Garrett Meigs, Dave Werntz, Ryan Haugo, and two anonymous reviewers. We also acknowledge financial support of the Pacific Northwest and Pacific Southwest Research Stations, the National Fire Plan, the Okanogan-Wenatchee National Forest, and the Joint Fire Sciences Program. NR 367 TC 6 Z9 6 U1 76 U2 113 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2016 VL 366 BP 221 EP 250 DI 10.1016/j.foreco.2016.01.034 PG 30 WC Forestry SC Forestry GA DK8EX UT WOS:000375161100021 ER PT J AU Huang, JY Henao, OL Griffin, PM Vugia, DJ Cronquist, AB Hurd, S Tobin-D'Angelo, M Ryan, P Smith, K Lathrop, S Zansky, S Cieslak, PR Dunn, J Holt, KG Wolpert, BJ Patrick, ME AF Huang, Jennifer Y. Henao, Olga L. Griffin, Patricia M. Vugia, Duc J. Cronquist, Alicia B. Hurd, Sharon Tobin-D'Angelo, Melissa Ryan, Patricia Smith, Kirk Lathrop, Sarah Zansky, Shelley Cieslak, Paul R. Dunn, John Holt, Kristin G. Wolpert, Beverly J. Patrick, Mary E. TI Infection with Pathogens Transmitted Commonly Through Food and the Effect of Increasing Use of Culture-Independent Diagnostic Tests on Surveillance - Foodborne Diseases Active Surveillance Network, 10 US Sites, 2012-2015 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Editorial Material ID UNITED-STATES; CRYPTOSPORIDIOSIS C1 [Huang, Jennifer Y.; Henao, Olga L.; Griffin, Patricia M.; Patrick, Mary E.] CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Vugia, Duc J.] Calif Dept Publ Hlth, Sacramento, CA USA. [Cronquist, Alicia B.] Colorado Dept Publ Hlth & Environm, Colorado Springs, CO USA. [Hurd, Sharon] Connecticut Dept Publ Hlth, Hartford, CT USA. [Tobin-D'Angelo, Melissa] Georgia Dept Publ Hlth, Atlanta, GA USA. [Ryan, Patricia] Maryland Dept Hlth & Mental Hyg, Baltimore, MD 21201 USA. [Smith, Kirk] Minnesota Dept Hlth, Minneapolis, MN 55414 USA. [Lathrop, Sarah] Univ New Mexico, Albuquerque, NM 87131 USA. [Zansky, Shelley] New York State Dept Hlth, Albany, NY 12237 USA. [Dunn, John] Tennessee Dept Hlth, Memphis, TN USA. [Holt, Kristin G.] USDA, Food Safety & Inspect Serv, Washington, DC USA. [Wolpert, Beverly J.] Food & Drug Adm, Ctr Food Safety & Appl Nutr, Atlanta, GA USA. RP Patrick, ME (reprint author), CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. EM mepatrick@cdc.gov NR 8 TC 4 Z9 4 U1 3 U2 7 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD APR 15 PY 2016 VL 65 IS 14 BP 368 EP 371 PG 4 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DK0WP UT WOS:000374633200002 PM 27077946 ER PT J AU Kawakami, VM Bottichio, L Angelo, K Linton, N Kissler, B Basler, C Lloyd, J Inouye, W Gonzales, E Rietberg, K Melius, B Oltean, H Wise, M Sinatra, J Marsland, P Li, Z Meek, R Kay, M Duchin, J Lindquist, S AF Kawakami, Vance M. Bottichio, Lyndsay Angelo, Kristina Linton, Natalie Kissler, Bonnie Basler, Colin Lloyd, Jennifer Inouye, Wendy Gonzales, Elysia Rietberg, Krista Melius, Beth Oltean, Hanna Wise, Matthew Sinatra, Jennifer Marsland, Paula Li, Zhen Meek, Roxanne Kay, Meagan Duchin, Jeff Lindquist, Scott TI Outbreak of Multidrug-Resistant Salmonella Infections Linked to Pork - Washington, 2015 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Editorial Material C1 [Kawakami, Vance M.; Lloyd, Jennifer; Inouye, Wendy; Gonzales, Elysia; Rietberg, Krista; Kay, Meagan; Duchin, Jeff] Publ Hlth Seattle & King Cty, Seattle, WA USA. [Kawakami, Vance M.; Angelo, Kristina] CDC, Epidem Intelligence Serv, Div Sci Educ & Profess Dev, Atlanta, GA 30333 USA. [Bottichio, Lyndsay; Angelo, Kristina; Basler, Colin; Wise, Matthew] CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Linton, Natalie; Melius, Beth; Oltean, Hanna; Lindquist, Scott] Washington State Dept Hlth, Off Communicable Dis Epidemiol, Shoreline, WA USA. [Kissler, Bonnie; Sinatra, Jennifer] USDA, Food Safety & Inspect Serv, Washington, DC 20250 USA. [Marsland, Paula; Li, Zhen; Meek, Roxanne] Washington State Dept Hlth, Publ Hlth Labs, Shoreline, WA USA. [Duchin, Jeff] Univ Washington, Med Ctr, Seattle, WA 98195 USA. RP Kawakami, VM (reprint author), Publ Hlth Seattle & King Cty, Seattle, WA USA.; Kawakami, VM (reprint author), CDC, Epidem Intelligence Serv, Div Sci Educ & Profess Dev, Atlanta, GA 30333 USA. EM vance.kawakami@kingcounty.gov NR 6 TC 1 Z9 1 U1 1 U2 2 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD APR 15 PY 2016 VL 65 IS 14 BP 379 EP 381 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA DK0WP UT WOS:000374633200005 PM 27078514 ER PT J AU Smith, JN Emlen, DJ Pearson, DE AF Smith, Jennifer N. Emlen, Douglas J. Pearson, Dean E. TI Linking Native and Invader Traits Explains Native Spider Population Responses to Plant Invasion SO PLOS ONE LA English DT Article ID ORB-WEB SPIDERS; PREDATOR ABUNDANCE; IMPACTS; DIVERSITY; RESOURCE; BEHAVIOR; COMMUNITIES; COMPETITION; LIMITATION; PATTERNS AB Theoretically, the functional traits of native species should determine how natives respond to invader-driven changes. To explore this idea, we simulated a large-scale plant invasion using dead spotted knapweed (Centaurea stoebe) stems to determine if native spiders' web-building behaviors could explain differences in spider population responses to structural changes arising from C. stoebe invasion. After two years, irregular web-spiders were >30 times more abundant and orb weavers were >23 times more abundant on simulated invasion plots compared to controls. Additionally, irregular web-spiders on simulated invasion plots built webs that were 4.4 times larger and 5.0 times more likely to capture prey, leading to >2-fold increases in recruitment. Orb-weavers showed no differences in web size or prey captures between treatments. Web-spider responses to simulated invasion mimicked patterns following natural invasions, confirming that C. stoebe's architecture is likely the primary attribute driving native spider responses to these invasions. Differences in spider responses were attributable to differences in web construction behaviors relative to historic web substrate constraints. Orb-weavers in this system constructed webs between multiple plants, so they were limited by the overall quantity of native substrates but not by the architecture of individual native plant species. Irregular web-spiders built their webs within individual plants and were greatly constrained by the diminutive architecture of native plant substrates, so they were limited both by quantity and quality of native substrates. Evaluating native species traits in the context of invader-driven change can explain invasion outcomes and help to identify factors limiting native populations. C1 [Smith, Jennifer N.; Emlen, Douglas J.; Pearson, Dean E.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Pearson, Dean E.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT USA. [Smith, Jennifer N.] Natl Ecol Observ Network NEON Inc, Prairie Peninsula Domain, Field Operat, Manhattan, KS USA. RP Smith, JN (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA.; Smith, JN (reprint author), Natl Ecol Observ Network NEON Inc, Prairie Peninsula Domain, Field Operat, Manhattan, KS USA. EM jsmith@neoninc.org OI Smith, Jennifer/0000-0001-8671-6103 FU Rocky Mountain Research Station; University of Montana; Sigma Xi; Mazamas Graduate Student Research Grant; USDA Forest Service FX This work was funded by the Rocky Mountain Research Station, USDA Forest Service (DEP); The University of Montana (JNS, DJE); Sigma Xi Grants-in-Aid of Research, https://www.sigmaxi.org/programs/grants-in-aid (JNS); and the Mazamas Graduate Student Research Grant, http://mazamas.org/resources/research-grants/ (JNS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 0 Z9 0 U1 4 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 15 PY 2016 VL 11 IS 4 AR e0153661 DI 10.1371/journal.pone.0153661 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ6AH UT WOS:000374291800038 PM 27082240 ER PT J AU Boutry, C El-Kadi, SW Suryawan, A Steinhoff-Wagner, J Stoll, B Orellana, RA Nguyen, HV Kimball, SR Fiorotto, ML Davis, TA AF Boutry, Claire El-Kadi, Samer W. Suryawan, Agus Steinhoff-Wagner, Julia Stoll, Barbara Orellana, Renan A. Nguyen, Hanh V. Kimball, Scot R. Fiorotto, Marta L. Davis, Teresa A. TI Pulsatile delivery of a leucine supplement during long-term continuous enteral feeding enhances lean growth in term neonatal pigs SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE leucine; growth; protein metabolism; orogastric feeding; infant ID MUSCLE PROTEIN-SYNTHESIS; AMINO-ACID AVAILABILITY; RAT SKELETAL-MUSCLE; FACTOR 2 EEF2; TRANSLATION INITIATION; DIETARY LEUCINE; DIFFERENTIAL REGULATION; INTERMITTENT BOLUS; SIGNAL INTEGRATION; INSULIN-RESISTANCE AB Neonatal pigs are used as a model to study and optimize the clinical treatment of infants who are unable to maintain oral feeding. Using this model, we have shown previously that pulsatile administration of leucine during continuous feeding over 24 h via orogastric tube enhanced protein synthesis in skeletal muscle compared with continuous feeding alone. To determine the long-term effects of leucine pulses, neonatal piglets (n = 11-12/group) were continuously fed formula via orogastric tube for 21 days, with an additional parenteral infusion of either leucine (CON + LEU; 800 mu mol.kg(-1).h(-1)) or alanine (CON + ALA) for 1 h every 4 h. The results show that body and muscle weights and lean gain were similar to 25% greater, and fat gain was 48% lower in CON + LEU than CON + ALA; weights of other tissues were unaffected by treatment. Fractional protein synthesis rates in longissimus dorsi, gastrocnemius, and soleus muscles were similar to 30% higher in CON + LEU compared with CON + ALA and were associated with decreased Deptor abundance and increased mTORC1, mTORC2, 4E-BP1, and S6K1 phosphorylation, SNAT2 abundance, and association of eIF4E with eIF4G and RagC with mTOR. There were no treatment effects on PKB, eIF2 alpha, eEF2, or PRAS40 phosphorylation, Rheb, SLC38A9, v-ATPase, LAMTOR1, LAMTOR2, RagA, RagC, and LAT1 abundance, the proportion of polysomes to nonpolysomes, or the proportion of mRNAs encoding rpS4 or rpS8 associated with polysomes. Our results demonstrate that pulsatile delivery of a leucine supplement during 21 days of continuous enteral feeding enhances lean growth by stimulating the mTORC1-dependent translation initiation pathway, leading to protein synthesis in skeletal muscle of neonates. C1 [Boutry, Claire; El-Kadi, Samer W.; Suryawan, Agus; Steinhoff-Wagner, Julia; Stoll, Barbara; Orellana, Renan A.; Nguyen, Hanh V.; Fiorotto, Marta L.; Davis, Teresa A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Kimball, Scot R.] Penn State Univ, Coll Med, Dept Cellular & Mol Physiol, Hershey, PA USA. RP Davis, TA (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institute of Child Health and Human Development [HD-072891]; USDA National Institute of Food and Agriculture [2013-67015-20438]; National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR-044474, AR-046308]; USDA Current Research Information System Grant [6250-51000-055] FX This work is a publication of the US Department of Agriculture/Agricultural Research Service (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine. Financial support for this project was provided by National Institute of Child Health and Human Development Grant HD-072891, USDA National Institute of Food and Agriculture Grant 2013-67015-20438, National Institute of Arthritis and Musculoskeletal and Skin Diseases Grants AR-044474 and AR-046308, and USDA Current Research Information System Grant 6250-51000-055. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 92 TC 1 Z9 1 U1 1 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD APR 15 PY 2016 VL 310 IS 8 BP E699 EP E713 DI 10.1152/ajpendo.00479.2015 PG 15 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA DJ3IV UT WOS:000374099100011 PM 26884386 ER PT J AU Wilson, GV Rigby, JR Ursic, M Dabney, SM AF Wilson, G. V. Rigby, J. R. Ursic, M. Dabney, S. M. TI Soil pipe flow tracer experiments: 1. Connectivity and transport characteristics SO HYDROLOGICAL PROCESSES LA English DT Article DE hillslope hydrology; flow velocity; breakthrough curve; preferential flow; connectivity ID PREFERENTIAL FLOW; CATCHMENT; MACROPORES; HILLSLOPE; FLUORESCEIN; EROSION; SYSTEMS; SEGMENT; RUNOFF; DESIGN AB Much debate has occurred in catchment hydrology regarding the connectivity of flow paths from upslope areas to catchment outlets. This study was conducted in two catchments, one with three upper branches, in a loess soil with a fragipan that fosters lateral flow and exhibits an extensive distribution of soil pipe collapse features. The study aimed to determine the connectivity of multiple soil pipe networks as well as determine pipe flow velocities during storm events. Fluorescein dye was injected directly into soil pipes at the upper most pipe collapse feature of four different hillslopes. Breakthrough curves (BTC) were determined by sampling multiple pipe collapse features downslope. The BTCs were used to determine the average' (centre of mass) and maximum' (first arrival) flow velocities. This study confirmed that these catchments contain individual continuous soil pipe networks that extend over 190m and connect the upper most hillslopes areas with the catchment outlet. While the flow paths are continuous, the individual pipe networks consist of alternating reaches of subsurface flow through soil pipes and reaches of surface flow through gullies formed by pipe collapses. In addition, flow can be occurring both through the subsurface soil pipes simultaneous with surface flow generated by artesian flow from the soil pipes. The pipe flow velocities were as high as 0.3m/s, which was in the range of streamflow velocities. These pipe flow velocities were also in the range of velocities observed in pinhole erosion tests suggesting that these large, mature soil pipes are still actively eroding. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Wilson, G. V.; Rigby, J. R.; Ursic, M.; Dabney, S. M.] USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, 598 McElroy Dr, Oxford, MS 38655 USA. RP Wilson, GV (reprint author), USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, 598 McElroy Dr, Oxford, MS 38655 USA. EM glenn.wilson@ars.usda.gov NR 46 TC 1 Z9 1 U1 4 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD APR 15 PY 2016 VL 30 IS 8 BP 1265 EP 1279 DI 10.1002/hyp.10713 PG 15 WC Water Resources SC Water Resources GA DJ1JQ UT WOS:000373959500008 ER PT J AU Zhou, Y Wilson, GV Fox, GA Rigby, JR Dabney, SM AF Zhou, Yan Wilson, G. V. Fox, G. A. Rigby, J. R. Dabney, S. M. TI Soil pipe flow tracer experiments: 2. Application of a streamflow transient storage zone model SO HYDROLOGICAL PROCESSES LA English DT Article DE solute transport; tracer injection; preferential flow; pipe network; breakthrough curve; tortuosity ID PREFERENTIAL FLOW; NUMERICAL-ANALYSIS; FORESTED HILLSLOPES; WATER EXCHANGE; MACROPORES; TRANSPORT; FLUORESCEIN; CATCHMENTS; EROSION; STATE AB Soil pipes are important subsurface flow pathways in many soil erosion phenomena. However, limited research has been performed on quantifying and characterizing their flow and transport characteristics. The objectives of this research were to determine the applicability of a streamflow model with transient storage in deriving flow and transport characteristics of soil pipes. Tracer data from pulse inputs were collected in four different soil pipes after a fluorescein dye was injected in the upstream end of each soil pipe network in three branches (west, middle, and east) of a main catchment and a back catchment in Goodwin Creek Experimental Watershed in Mississippi. Multiple sampling stations were positioned along each soil pipe network. The transient storage zone model OTIS-P was executed inversely to estimate transport parameters by soil pipe reach such as the soil pipe cross-sectional area (A), soil storage zone cross-sectional area (A(s)), and exchange rate between the soil pipe and the soil storage zone ((s)). Model convergence was achieved, and simulated breakthrough curves of the reaches were in good agreement with actual tracer data for eight of the nine reaches of the three branches of the Main Catchment and five of the seven reaches of the Back Catchment soil pipe. Simulation parameters for the soil pipe networks were similar to the range of values reported for flow and transport characteristics commonly observed in streams. Inversely, estimated soil pipe flow velocities were higher with increased tortuosity, which led to a smaller cross-sectional areas predicted for the soil pipe flowpaths, while other parameters were not sensitive to tortuosity. In general, application of One-Dimensional Transport with Inflow and Storage-P to this unique soil pipe condition suggested larger transient storage (A(s) and (s)) compared with most stream systems. This was hypothesized to be because of relatively higher ratio of the wetted perimeter to flow area in the soil pipe, the hydraulic roughness of the soil pipe, potential retention in collapsed portions of the pipe, and interaction with smaller preferential flow systems. Copyright (c) 2015 John Wiley & Sons, Ltd. C1 [Zhou, Yan; Fox, G. A.] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. [Wilson, G. V.; Rigby, J. R.; Dabney, S. M.] USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Fox, G. A.] Dept Biosyst & Agr Engn, Stillwater, OK USA. [Fox, G. A.] Oklahoma Water Resources Ctr, Stillwater, OK USA. RP Wilson, GV (reprint author), USDA ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, Oxford, MS 38655 USA. EM Glenn.Wilson@ARS.USDA.GOV FU National Science Foundation [0943491]; China Scholarship Council (CSC) [201306306300023]; Buchanan Family Trust through Buchanan Endowed Chair at Oklahoma State University FX This research is partly based upon work supported by the National Science Foundation under grant no. 0943491. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The authors also acknowledge the Buchanan Family Trust by providing funds through the Buchanan Endowed Chair at Oklahoma State University. The author Y Zhou is supported in part by the scholarship from China Scholarship Council (CSC) under the Grant CSC No. 201306306300023. NR 43 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD APR 15 PY 2016 VL 30 IS 8 BP 1280 EP 1291 DI 10.1002/hyp.10712 PG 12 WC Water Resources SC Water Resources GA DJ1JQ UT WOS:000373959500009 ER PT J AU Hruby, CE Soupir, ML Moorman, TB Shelley, M Kanwar, RS AF Hruby, C. E. Soupir, M. L. Moorman, T. B. Shelley, M. Kanwar, R. S. TI Effects of tillage and poultry manure application rates on Salmonella and fecal indicator bacteria concentrations in tiles draining Des Moines Lobe soils SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Fecal indicator bacteria; Salmonella spp.; Poultry manure; Tile drainage ID ENVIRONMENTAL FARM SAMPLES; LIQUID SWINE MANURE; ESCHERICHIA-COLI; E. COLI; LITTER APPLICATION; WATER-QUALITY; RECREATIONAL WATER; AGRICULTURAL SOIL; DRAINAGE WATER; UNITED-STATES AB Application of poultry manure (PM) to cropland as fertilizer is a common practice in artificially drained regions of the Upper Midwest United States. Tile-waters have the potential to contribute pathogenic bacteria to downstream waters. This 3-year study (2010-2012) was designed to evaluate the impacts of manure management and tillage practices on bacteria losses to drainage tiles under a wide range of field conditions. PM was applied annually in spring, prior to planting corn, at application rates ranging from 5 to 40 kg/ha to achieve target rates of 112 and 224 kg/ha nitrogen (PM1 and PM2). Control plots received no manure (PMO). Each treatment was replicated on three chisel-plowed (CP) plots and one no-till (NT) plot. Tile-water grab samples were collected weekly when tiles were flowing beginning 30 days before manure application to 100 days post application, and additional grab samples were obtained to target the full spectrum of flow conditions. Manure and tile-water samples were analyzed for the pathogen, Salmonella spp. (SALM), and fecal indicator bacteria (FIB), Escherichia coli (EC), and enterococci (ENT). All three bacterial genera were detected more frequently, and at significantly higher concentrations, in tile waters draining NT plots compared to CP plots. Transport of bacteria to NT tiles was most likely facilitated by macropores, which were significantly more numerous above tiles in NT plots in 2012 as determined by smoke-testing. While post-manure samples contained higher concentrations of bacteria than pre-manure samples, significant differences were not seen between low (PM1) and high (PM2) rates of PM application. The highest concentrations were observed under the NT PM2 plot in 2010 (6.6 x 10(3) cfu/100 mL EC, 6.6 x 10(5) cfu/100 mL ENT, and 2.8 x 10(3) cfu/100 mL SALM). Individual and 30-day geometric mean ENT concentrations correlated more strongly to SALM than EC; however, SALM were present in samples with little or no FIB. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Hruby, C. E.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. [Soupir, M. L.] Iowa State Univ, Dept Agr & Biosyst Engn, 3358 Elings Hall, Ames, IA 50011 USA. [Moorman, T. B.] USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. [Shelley, M.] Iowa State Univ, Dept Stat, 509 Ross Hall, Ames, IA 50011 USA. [Shelley, M.] Iowa State Univ, Dept Polit Sci, 509 Ross Hall, Ames, IA 50011 USA. [Kanwar, R. S.] Iowa State Univ, Dept Agr & Biosyst Engn, 4358 Elings Hall, Ames, IA 50011 USA. RP Soupir, ML (reprint author), Iowa State Univ, Dept Agr & Biosyst Engn, 3358 Elings Hall, Ames, IA 50011 USA. EM msoupir@iastate.edu FU Iowa State University; Iowa Egg Council FX This project was funded by Iowa State University and a 3-year grant from the Iowa Egg Council as part of a long-term study of the impacts of PM application on water quality. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the IEC. The authors would like to thank Carl Pederson, Beth Douglass, Ross Tuttle, Kendall Agee, Mandy Homan, Samantha Riess, Natasha Hoover, Pramod Pandey, Rohith Gali, Jason Garder, Javier Guzman, Charles Ikenberry, Haider Qleibo, Nick Terhall, and Conrad Brendel for assistance with data collection and laboratory analysis. Valuable manuscript review was provided by Dr. Robert Ewing. NR 59 TC 1 Z9 1 U1 5 U2 9 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD APR 15 PY 2016 VL 171 BP 60 EP 69 DI 10.1016/j.jenvman.2016.01.040 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DH3IO UT WOS:000372680900008 PM 26874615 ER PT J AU Nunez, A Lehotay, SJ Lightfield, AR AF Nunez, Alberto Lehotay, Steven J. Lightfield, Alan R. TI Structural characterization of product ions of regulated veterinary drugs by electrospray ionization and quadrupole time-of-flight mass spectrometry. Part 3: Anthelmintics and thyreostats SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID PERFORMANCE LIQUID-CHROMATOGRAPHY; RESIDUES; FRAGMENTATION; VALIDATION AB RationalePreviously, we have reported a liquid chromatography/tandem mass spectrometry method for the identification and quantification of regulated veterinary drugs in food animals. The method uses three selected transition ions per analyte but structural characterization is also needed. This work is a continuation of two previous publications in which we propose structures of the selected transition ions of 130 veterinary drugs altogether. MethodsIn this work, 24 additional veterinary drugs were analyzed by infusion into a high-resolution quadrupole time-of-flight (QTOF) mass spectrometer using electrospray ionization (ESI) in positive or negative mode. The TOF analyzer was calibrated to achieve low error mass accuracy in the MS and MS/MS modes. Also, the MS2 and MS3 spectra were obtained by using a Q-Trap mass spectrometer to further determine the possible pathways of ion formation. ResultsThe low error mass spectrometry analysis allowed the elucidation of the ion formulae of selected transition ions for qualitative identification. The rational interpretation of data including a review of the published literature led to the proposed structures of the MS/MS product ions of 24 compounds covering two classes of regulated veterinary drugs (anthelmintics and thyreostats). In addition, the use of MS2 and MS3 experiments led to the establishment of fragmentation patterns. ConclusionsThe identification and quantification of veterinary drug residues is helpful information for regulatory monitoring programs in defense of regulatory enforcement actions. Published in 2016. This article is a U.S. Government work and is in the public domain in the USA. C1 [Nunez, Alberto; Lehotay, Steven J.; Lightfield, Alan R.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Nunez, A (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM alberto.nunez@ars.usda.gov FU Agricultural Research Service [60-1935-9-031]; Food Safety and Inspection Service [60-1935-9-031] FX The authors would like to thank Leo Sapozhnikov for his voluntary work in this research. This work was conducted under Interagency Agreement No. #60-1935-9-031 between the Agricultural Research Service and the Food Safety and Inspection Service. Mention of brand or firm name does not constitute an endorsement by the US Department of Agriculture above others of a similar nature not mentioned. USDA is an equal opportunity provider and employer. NR 22 TC 0 Z9 0 U1 1 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0951-4198 EI 1097-0231 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PD APR 15 PY 2016 VL 30 IS 7 BP 813 EP 822 DI 10.1002/rcm.7508 PG 10 WC Biochemical Research Methods; Chemistry, Analytical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA DH0XS UT WOS:000372508100004 PM 26969922 ER PT J AU Zhang, XC Liu, BL AF Zhang, X. C. (John) Liu, B. L. TI Using multiple composite fingerprints to quantify fine sediment source contributions: A new direction SO GEODERMA LA English DT Article DE Sediment source fingerprinting; Tracer selection; Composite fingerprints; Mixing model; Model optimization ID SUSPENDED SEDIMENT; RIVER-BASINS; UK; CATCHMENTS; AUSTRALIA; TRACERS; PB-210 AB Sediment source fingerprinting provides an essential means for estimating sediment source contributions, which are needed not only for soil conservation planning but also for erosion model evaluation/refinement. A single optimum composite fingerprint has been widely used in the literature to estimate sediment provenance. The objectives of this work are to (1) verify whether an optimum composite fingerprint exists, (2) present a new direction of using multiple composite fingerprints to improve the accuracy and reliability of source contribution estimation, and (3) evaluate the optimization model formulation and the validity of the tracer discriminatory weighting. This study shows that tracer selection greatly impacts the estimated source contributions. The optimum composite fingerprint may not exist, or at least cannot be identified simply based on ability of the tracer to discriminate sources because of the lack of correlation between ability of the tracer to discriminate and its rigor in estimating source contributions. The weak link is likely caused by (1) tracer conflicts, (2) differential tracer measurement errors, and (3) varying degree of the conservativeness of each tracer or lack of it. To overcome this shortcoming, a new approach of using multiple composite fingerprints was proposed. Contrary to the popular tracer reduction strategies, this new approach uses a maximum number of composite fingerprints, which contain non-contradictory tracers in each composite, to maximize the use of all tracer information. The new approach assumes that source proportions averaged over multiple composite fingerprints are more likely to be closer to the population means than any estimate using a single fingerprint alone. Such a 'mean of the means' approach has been shown to not only improve the accuracy but also reduce the uncertainty of the proportion estimates. The model of the absolute relative difference performed slightly better than the squared relative difference model in estimating source contributions, suggesting the former be preferred. The results also indicated that the tracer discriminatory weighting should be excluded as it tends to bias contribution estimates. Published by Elsevier B.V. C1 [Zhang, X. C. (John)] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. [Liu, B. L.] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou, Gansu, Peoples R China. [Liu, B. L.] 320 Donggangxi Rd, Lanzhou 730000, Gansu, Peoples R China. RP Zhang, XC (reprint author), 7207 W Cheyenne St, El Reno, OK 73036 USA. EM john.zhang@ars.usda.gov NR 42 TC 4 Z9 4 U1 9 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD APR 15 PY 2016 VL 268 BP 108 EP 118 DI 10.1016/j.geoderma.2016.01.031 PG 11 WC Soil Science SC Agriculture GA DF5CO UT WOS:000371369600013 ER PT J AU Hill, BH Jicha, TM Lehto, LLP Elonen, CM Sebestyen, SD Kolka, RK AF Hill, Brian H. Jicha, Terri M. Lehto, LaRae L. P. Elonen, Colleen M. Sebestyen, Stephen D. Kolka, Randall K. TI Comparisons of soil nitrogen mass balances for an ombrotrophic bog and a minerotrophic fen in northern Minnesota SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Bog; Denitrification; Fen; Peat; Soils; Watershed N budget ID FRESH-WATER WETLANDS; ORGANIC-MATTER; ACETYLENE INHIBITION; FORESTED WATERSHEDS; AQUATIC ECOSYSTEMS; CARBON-CYCLE; PORE-WATER; PEAT BOG; DENITRIFICATION; PEATLANDS AB We compared nitrogen (N) storage and flux in soils from an ombrotrophic bog with that of a minerotrophic ten to quantity the differences in N cycling between these two peatlands types in northern Minnesota (USA). Precipitation, atmospheric deposition, and bog and ten outflows were analyzed for nitrogen species. Upland and peatland soil samples were analyzed for N content, and for ambient (DN) and potential (DEA) denitrification rates. Annual atmospheric deposition was: 0.88-3.07 kg NH4+ ha(-1) y(-1); 1.37-1.42 kg NO3- ha(-1) y(-1); 2.79-4.69 kg TN ha(-1) y(-1). Annual N outflows were: bog -0.01-0.04 kg NH4+ ha(-1) y(-1), NO3- 0.01-0.06 kg ha(-1) y(-1) and TN 0.11-0.69 kg ha(-1) y(-1); fen-NH4+ 0.01-0.16 kg ha(-1) y(-1), NO3- 0.29-0.48 kg ha(-1) y(-1), and TN 1.14-1.61 kg ha(-1) y(-1). Soil N content depended on location within the bog or fen, and on soil depth. DN and DEA rates were low throughout the uplands and peatlancls, and were correlated with atmospheric N deposition, soil N storage, and N outflow. DEA was significantly greater than DN indicating C or N limitation of the denitrification process. We highlight differences between the bog and fen, between the upland mineral soils and peat, and the importance of biogeochemical hotspots within the peatlands. We point out the importance of organic N storage, as a source of N for denitrification, and propose a plausible link between organic N storage, denitrification and N export from peatlands. Finally, we considered the interactions of microbial metabolism with nutrient availability and stoichiometry, and how N dynamics might be affected by climate change in peatland ecosystems. Published by Elsevier B.V. C1 [Hill, Brian H.; Jicha, Terri M.; Lehto, LaRae L. P.; Elonen, Colleen M.] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Lab, Midcontinent Ecol Div, Duluth, MN 55804 USA. [Sebestyen, Stephen D.; Kolka, Randall K.] US Forest Serv, No Res Stn, Ctr Res Ecosyst Change, Grand Rapids, MN 55744 USA. [Lehto, LaRae L. P.] AMI Consulting Engineers, Superior, WI USA. RP Hill, BH (reprint author), USEPA NHEERL MED, 6201 Congdon Blvd, Duluth, MN 55804 USA. EM hill.brian@epa.gov RI Sebestyen, Stephen/D-1238-2013 OI Sebestyen, Stephen/0000-0002-6315-0108 NR 60 TC 2 Z9 2 U1 8 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 15 PY 2016 VL 550 BP 880 EP 892 DI 10.1016/j.scitotenv.2016.01.178 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA DF3EM UT WOS:000371226700087 PM 26851760 ER PT J AU Luby, EM Moorman, TB Soupir, ML AF Luby, Elizabeth M. Moorman, Thomas B. Soupir, Michelle L. TI Fate and transport of tylosin-resistant bacteria and macrolide resistance genes in artificially drained agricultural fields receiving swine manure SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Antibiotic resistance; erm genes; Enterococcos; Tile drainage; Manure; Swine; Soil ID ANTIBIOTIC-RESISTANCE; STREPTOGRAMIN-B; PCR ASSAYS; ERM GENES; SOIL; QUALITY; WATER; DETERMINANTS; LINCOSAMIDE; ENTEROCOCCI AB Application of manure from swine treated with antibiotics introduces antibiotics and antibiotic resistance genes to soil with the potential for further movement in drainage water, which may contribute to the increase in antibiotic resistance in non-agricultural settings. We compared losses of antibiotic-resistant Enterococcus and macrolide-resistance (erm and msrA) genes in water draining from plots with or without swine manure application under chisel plow and no till conditions. Concentrations of ermB, ermC and ermf were all >10(9) copies g(-1) in manure from tylosin-treated swine, and application of this manure resulted in short-term increases in the abundance of these genes in soil. Abundances of ermB, ermC and ermF in manured soil returned to levels identified in non-manured control plots by the spring following manure application. Tillage practices yielded no significant differences (p > 0.10) in enterococci or erm gene concentrations in drainage water and were therefore combined for further analysis. While enterococci and tylosin-resistant enterococci concentrations in drainage water showed no effects of manure application, ermB and ermF concentrations in drainage water from manured plots were significantly higher (p < 0.01) than concentrations coming from non-manured plots. ErmB and ermF were detected in 78% and 44%, respectively, of water samples draining from plots receiving manure. Although ermC had the highest concentrations of the three genes in drainage water, there was no effect of manure application on ermC abundance. MsrA was not detected in manure, soil or water. This study is the first to report significant increases in abundance of resistance genes in waters draining from agricultural land due to manure application. (C) 2016 Published by Elsevier B.V. C1 [Luby, Elizabeth M.; Soupir, Michelle L.] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. [Moorman, Thomas B.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA USA. RP Luby, EM (reprint author), Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA USA. FU National Pork Board [13-051] FX Funding for this research was wholly supported by grant 13-051 from the National Pork Board. NR 42 TC 1 Z9 1 U1 9 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 15 PY 2016 VL 550 BP 1126 EP 1133 DI 10.1016/j.scitotenv.2016.01.132 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DF3EM UT WOS:000371226700111 PM 26874610 ER PT J AU Yan, WJ Yang, LP Zhuang, H Wu, HZ Zhang, JH AF Yan, Wenjing Yang, Longping Zhuang, Hong Wu, Haizhou Zhang, Jianhao TI Engineered "hot" core-shell nanostructures for patterned detection of chloramphenicol SO BIOSENSORS & BIOELECTRONICS LA English DT Article DE Chloramphenicol; Core-shell nanostructure; Aptamer; SERS; Cy5 dye ID ENHANCED RAMAN-SPECTROSCOPY; GOLD NANOPARTICLES; DNA DETECTION; SCATTERING; METRONIDAZOLE; AMPLIFICATION; IMMUNOASSAY; APTASENSOR; GROWTH; DIMERS AB In this study, we described a novel method for highly sensitive and specific detection of chloramphenicol (CAP) based on engineered "hot" Au core-Ag shell nanostructures (Au@Ag NSs). Cy5-labeled DNA aptamer was embedded between the Au and Ag layers as a signal generator and target-recognition element, to fabricate uniform Au@Ag NSs with unexpected strong and stable SERS signals. The presented CAP can specifically bind to the DNA aptamer by forming an aptamer-CAP conjugate, and cause greatly decreased SERS signals of Au@Ag NSs. By using this method, we were able to detect as low as 0.19 pg mL(-1) of CAP with high selectivity, which is much lower than those previously reported biosensors. Compared with the other SERS sensors that attached a dye in the outer layer of nanoparticles, this method exhibits excellent sensitivity and has the potential to significantly improve stability and reproducibility of SERS-based detection techniques. (C) 2015 Elsevier B.V. All rights reserved. C1 [Yan, Wenjing; Yang, Longping; Wu, Haizhou; Zhang, Jianhao] Nanjing Agr Univ, Coll Food Sci & Technol, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. [Zhuang, Hong] ARS, Qual & Safety Assessment Res Unit, USDA, Athens, GA 30605 USA. RP Yan, WJ; Zhang, JH (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. EM ywj1103@njau.edu.cn; nau_zjh@njau.edu.cn FU Fundamental Research Funds for the Central Universities [0806J0452]; National Science & Technology Pillar Program [2015BAD16B00]; International Technology Cooperation Program of Jiangsu province, China [BZ2014034] FX This study was sponsored by the Fundamental Research Funds for the Central Universities (Grant no. 0806J0452); the National Science & Technology Pillar Program (Grant no. 2015BAD16B00); the International Technology Cooperation Program of Jiangsu province, China (Grant no. BZ2014034). NR 40 TC 4 Z9 4 U1 38 U2 153 PU ELSEVIER ADVANCED TECHNOLOGY PI OXFORD PA OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-5663 EI 1873-4235 J9 BIOSENS BIOELECTRON JI Biosens. Bioelectron. PD APR 15 PY 2016 VL 78 BP 67 EP 72 DI 10.1016/j.bios.2015.11.011 PG 6 WC Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology SC Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics GA DC1CR UT WOS:000368954900010 PM 26594888 ER PT J AU Majeed, H Liu, F Hategekimana, J Sharif, HR Qi, J Ali, B Bian, YY Ma, JG Yokoyama, W Zhong, F AF Majeed, Hamid Liu, Fei Hategekimana, Joseph Sharif, Hafiz Rizwan Qi, Jing Ali, Barkat Bian, Yuan-Yuan Ma, Jianguo Yokoyama, Wallace Zhong, Fang TI Bactericidal action mechanism of negatively charged food grade clove oil nanoemulsions SO FOOD CHEMISTRY LA English DT Article DE Clove oil; Anionic; Food grade nanoemulsion; Antimicrobial; Gram positive bacteria ID HIGH-PRESSURE HOMOGENIZATION; PLANT ESSENTIAL OILS; ANTIMICROBIAL ACTIVITY; LISTERIA-MONOCYTOGENES; ANTIBACTERIAL ACTIVITY; STABILITY EVALUATION; BETA-CYCLODEXTRIN; NANOPARTICLES; EMULSIONS; STAPHYLOCOCCUS AB Clove oil (CO) anionic nanoemulsions were prepared with varying ratios of CO to canola oil (CA), emulsified and stabilized with purity gum ultra (PGU), a newly developed succinylated waxy maize starch. Interfacial tension measurements showed that CO acted as a co-surfactant and there was a gradual decrease in interfacial tension which favored the formation of small droplet sizes on homogenization until a critical limit (5: 5% v/v CO: CA) was reached. Antimicrobial activity of the negatively charged CO nanoemulsion was determined against Gram positive GPB (Listeria monocytogenes and Staphylococcus aureus) and Gram negative GNB (Escherichia coli) bacterial strains using minimum inhibitory concentration (MIC) and a time kill dynamic method. Negatively charged PGU emulsified CO nanoemulsion showed prolonged antibacterial activities against Gram positive bacterial strains. We concluded that negatively charged CO nanoemulsion droplets self-assemble with GPB cell membrane, and facilitated interaction with cellular components of bacteria. Moreover, no electrostatic interaction existed between negatively charged droplets and the GPB membrane. (C) 2015 Elsevier Ltd. All rights reserved. C1 [Majeed, Hamid; Liu, Fei; Hategekimana, Joseph; Sharif, Hafiz Rizwan; Qi, Jing; Ali, Barkat; Bian, Yuan-Yuan; Ma, Jianguo; Zhong, Fang] Jiangnan Univ, Sch Food Sci & Technol, Key Lab Food Colloids & Biotechnol, Minist Educ, Wuxi 214122, Peoples R China. [Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Key Lab Food Colloids & Biotechnol, Minist Educ, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn FU National 863 Program [2011BAD23B02, 2013AA102207]; NSFC - China [31171686, 30901000]; 111 project [B07029]; [PCSIRT0627] FX This work was financially supported by National 863 Program 2011BAD23B02, 2013AA102207, NSFC - China, 31171686, 30901000, 111 project-B07029 and PCSIRT0627. NR 41 TC 5 Z9 5 U1 12 U2 231 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD APR 15 PY 2016 VL 197 BP 75 EP 83 DI 10.1016/j.foodchem.2015.10.015 PN A PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA CZ3DZ UT WOS:000366985900010 PM 26616926 ER PT J AU Ramsay, TG Stoll, MJ Blomberg, LA Caperna, TJ AF Ramsay, Timothy G. Stoll, Margo J. Blomberg, Le Ann Caperna, Thomas J. TI Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue SO JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY LA English DT Article DE Adipose; Cytokines; Neonate; Orosomucoid; Swine ID ALPHA-1-ACID GLYCOPROTEIN; ALPHA(1)-ACID GLYCOPROTEIN; DEVELOPMENTAL REGULATION; GLUCOSE-METABOLISM; MESSENGER-RNA; NONFAT CELLS; INFLAMMATION; PROTEIN; PIGS; SERUM AB Background: Porcine adipose tissue expresses orosomucoid (ORM1) mRNA, a protein with anti-inflammatory and immunomodulatory properties. Previous research has demonstrated that porcine ORM1 can reduce insulin stimulated glucose metabolism in porcine adipose tissue in vitro. The present study was designed to examine the preweaning ontogeny of ORM1 mRNA abundance in porcine subcutaneous adipose and to determine if ORM1 can regulate mRNA abundance of inflammatory cytokines that contribute to insulin resistance in primary cultures derived from neonatal porcine subcutaneous adipose tissue. Cultures were differentiated in vitro and subsequently the adipocyte containing cultures were incubated for 24 h with 0-5000 ng porcine ORM1/mL medium. Cultures were then harvested, total RNA extracted for use in reverse transcription and the mRNA abundance of cytokine mRNA quantified by real-time PCR. Results: ORM1 mRNA abundance within neonatal adipose tissue does not change from d 1 to d 21 of age and is a very small fraction relative to liver mRNA abundance. The ORM1 mRNA level in porcine adipocytes and stromal-vascular cells are similar (P > 0.05). Treatment with ORM1 did not affect TNF alpha (tumor necrosis factor a) mRNA level (P > 0.05), while interleukin 6 (IL6) mRNA abundance was reduced 32 % at 1,000 ng ORM1/mL (P < 0.01). However, TNFa protein content in the cell culture media was reduced by ORM1 treatment (5,000 ng/mL, P < 0.05), whereas ORM1 had no detectable effect on the media content of IL6 (P > 0.05). The reduction of macrophage migration inhibitory factor (MIF) mRNA abundance by ORM1 was dose dependent (P < 0.01). Monocyte chemotactic protein (MCP) mRNA level was reduced 27 % by 1,000 ng ORM1/mL (P < 0.05). Conclusions: The data suggest that ORM1 has limited effects TNF alpha, IL6, MIF or MCP expression at the concentrations tested. Secondly, these cytokines do not appear to contribute to the reported insulin resistance induced by ORM1 in porcine adipose tissue in vitro as an increase in the abundance of these inflammatory cytokines would be predicted during an insulin resistant state. C1 [Ramsay, Timothy G.; Stoll, Margo J.; Blomberg, Le Ann; Caperna, Thomas J.] USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Ramsay, TG (reprint author), USDA ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM timothy.ramsay@ars.usda.gov NR 35 TC 0 Z9 0 U1 2 U2 3 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2049-1891 J9 J ANIM SCI BIOTECHNO JI J. Anim. Sci. Biotechnol. PD APR 14 PY 2016 VL 7 AR 25 DI 10.1186/s40104-016-0081-0 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DK4BZ UT WOS:000374863000001 PM 27087941 ER PT J AU Sommers, CH Scullen, OJ Sheen, S AF Sommers, Christopher H. Scullen, O. J. Sheen, Shiowshuh TI Inactivation of Uropathogenic Escherichia coli in Ground Chicken Meat Using High Pressure Processing and Gamma Radiation, and in Purge and Chicken Meat Surfaces by Ultraviolet Light SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE UPEC; high pressure processing; gamma radiation; ultraviolet light; chicken ID ANTIMICROBIAL-RESISTANT; IRRADIATION TEMPERATURE; FOODBORNE ILLNESS; ELECTRON-BEAM; BEEF; SURVIVAL; O157-H7; HUMANS; RESERVOIR; POULTRY AB Extraintestinal pathogenic Escherichia coli, including uropathogenic E. coli (UPEC), are common contaminants in poultry meat and may cause urinary tract infections after colonization of the gastrointestinal tract and transfer of contaminated feces to the urethra. Three non-thermal processing technologies used to improve the safety and shelf-life of both human and pet foods include high pressure processing (HPP), ionizing (gamma) radiation (GR), and ultraviolet light (UV-C). Multi-isolate cocktails of UPEC were inoculated into ground chicken which was then treated with HPP (4 degrees C, 025 min) at 300, 400, or 500 MPa. HPP D10, the processing conditions needed to inactivate 1 log of UPEC, was 30.6, 8.37, and 4.43 min at 300, 400, and 500 MPa, respectively. When the UPEC was inoculated into ground chicken and gamma irradiated (4 and -20 degrees C) the GR D10 were 0.28 and 0.36 kGy, respectively. The UV-C D10 of UPEC in chicken suspended in exudate and placed on stainless steel and plastic food contact surfaces ranged from 11.4 to 12.9 mJ/cm2. UV-C inactivated ca. 0.6 log of UPEC on chicken breast meat. These results indicate that existing non-thermal processing technologies such as HPP, GR, and UV-C can significantly reduce UPEC levels in poultry meat or exudate and provide safer poultry products for at-risk consumers. C1 [Sommers, Christopher H.; Scullen, O. J.; Sheen, Shiowshuh] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA. RP Sommers, CH (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA USA. EM christopher.sommers@ars.usda.gov FU USDA-ARS National Program 108 Food Safety Project [8072-42000-073-00D] FX This Project was funded by USDA-ARS National Program 108 Food Safety Project No. 8072-42000-073-00D. NR 37 TC 2 Z9 2 U1 8 U2 13 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD APR 14 PY 2016 VL 7 AR 413 DI 10.3389/fmicb.2016.00413 PG 6 WC Microbiology SC Microbiology GA DJ1RN UT WOS:000373981100001 PM 27148167 ER PT J AU Fugate, KK Ribeiro, WS Lulai, EC Deckard, EL Finger, FL AF Fugate, Karen K. Ribeiro, Wellington S. Lulai, Edward C. Deckard, Edward L. Finger, Fernando L. TI Cold Temperature Delays Wound Healing in Postharvest Sugarbeet Roots SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Beta vulgaris; harvest injury; lignin; respiration; storage; suberin ID SOLANUM-TUBEROSUM L.; CHLOROGENIC ACID ISOMERS; POLYPHENOL OXIDASE; POTATO-TUBER; ANTIOXIDANT CAPACITY; PHENOLIC CONTENT; SUBERIN DEPOSITION; LIGNIN; STORAGE; TISSUE AB Storage temperature affects the rate and extent of wound-healing in a number of root and tuber crops. The effect of storage temperature on wound-healing in sugarbeet (Beta vulgaris L.) roots, however, is largely unknown. Wound-healing of sugarbeet roots was investigated using surface-abraded roots stored at 6 and 12 degrees C for 28 days. Surface abrasions are common injuries of stored roots, and the storage temperatures used are typical of freshly harvested or rapidly cooled roots. Transpiration rate from the wounded surface and root weight loss were used to quantify wound healing. At 12 degrees C, transpiration rate from the wounded surface declined within 14 days and wounded roots lost weight at a rate similar to unwounded controls. At 6 degrees C, transpiration rate from the wounded surface did not decline in the 28 days after injury, and wounded roots lost 44% more weight than controls after 28 days storage. Melanin formation, lignification, and suberization occurred more rapidly at 12 degrees C than at 6 degrees C, and a continuous layer of lignified and suberized cells developed at 12 degrees C, but not at 6 degrees C. Examination of enzyme activities involved in melanin, lignin, and suberin formation indicated that differences in melanin formation at 6 and 12 degrees C were related to differences in polyphenol oxidase activity, although no relationships between suberin or lignin formation and phenylalanine ammonia lyase or peroxidase activity were evident. Wound-induced respiration was initially greater at 12 degrees C than at 6 degrees C. However, with continued storage, respiration rate of wounded roots declined more rapidly at 12 degrees C, and over 28 days, the increase in respiration due to injury was 52% greater in roots stored at 6 degrees C than in roots stored at 12 degrees C. The data indicate that storage at 6 degrees C severely slowed and impaired wound-healing of surface-abraded sugarbeet roots relative to roots stored at 12 degrees C and suggest that postharvest losses may be accelerated if freshly harvested roots are cooled too quickly. C1 [Fugate, Karen K.; Lulai, Edward C.] ARS, Northern Crop Sci Lab, USDA, Fargo, ND USA. [Ribeiro, Wellington S.; Finger, Fernando L.] Univ Fed Vicosa, Dept Fitotecnia, Vicosa, MG, Brazil. [Deckard, Edward L.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. RP Fugate, KK (reprint author), ARS, Northern Crop Sci Lab, USDA, Fargo, ND USA. EM karen.fugate@ars.usda.gov FU Beet Sugar Development Foundation; CNPq; FAPEMIG of Brazil FX This work was supported in part by the Beet Sugar Development Foundation. Financial support for WR and FF was provided by CNPq and FAPEMIG of Brazil. NR 65 TC 0 Z9 0 U1 12 U2 22 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD APR 14 PY 2016 VL 7 AR 499 DI 10.3389/fpls.2016.00499 PG 14 WC Plant Sciences SC Plant Sciences GA DJ1OG UT WOS:000373972100001 PM 27148322 ER PT J AU Ziska, LH Pettis, JS Edwards, J Hancock, JE Tomecek, MB Clark, A Dukes, JS Loladze, I Polley, HW AF Ziska, Lewis H. Pettis, Jeffery S. Edwards, Joan Hancock, Jillian E. Tomecek, Martha B. Clark, Andrew Dukes, Jeffrey S. Loladze, Irakli Polley, H. Wayne TI Rising atmospheric CO2 is reducing the protein concentration of a floral pollen source essential for North American bees SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE bee health; carbon dioxide; Solidago canadensis; planetary health; pollen; protein ID APIS-MELLIFERA L.; HONEY-BEE; ELEVATED CO2; HUMAN-NUTRITION; PLANT-TISSUES; HYMENOPTERA; APIDAE; DIET; METAANALYSIS; TRANSPIRATION AB At present, there is substantive evidence that the nutritional content of agriculturally important food crops will decrease in response to rising levels of atmospheric carbon dioxide, C-a. However, whether C-a-induced declines in nutritional quality are also occurring for pollinator food sources is unknown. Flowering late in the season, goldenrod (Solidago spp.) pollen is a widely available autumnal food source commonly acknowledged by apiarists to be essential to native bee (e.g. Bombus spp.) and honeybee (Apis mellifera) health and winter survival. Using floral collections obtained from the Smithsonian Natural History Museum, we quantified C-a-induced temporal changes in pollen protein concentration of Canada goldenrod (Solidago canadensis), the most widespread Solidago taxon, from hundreds of samples collected throughout the USA and southern Canada over the period 1842-2014 (i.e. a C-a from approx. 280 to 398 ppm). In addition, we conducted a 2 year in situ trial of S. canadensis populations grown along a continuous C-a gradient from approximately 280 to 500 ppm. The historical data indicated a strong significant correlation between recent increases in Ca and reductions in pollen protein concentration (r(2) = 0.81). Experimental data confirmed this decrease in pollen protein concentration, and indicated that it would be ongoing as C-a continues to rise in the near term, i.e. to MO ppm (r(2) = 0.88). While additional data are needed to quantify the subsequent effects of reduced protein concentration for Canada goldenrod on bee health and population stability, these results are the first to indicate that increasing C-a can reduce protein content of a floral pollen source widely used by North American bees. C1 [Ziska, Lewis H.; Tomecek, Martha B.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Pettis, Jeffery S.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. [Edwards, Joan; Hancock, Jillian E.] Williams Coll, Dept Biol, Williamstown, MA 01267 USA. [Clark, Andrew] Smithsonian Inst, US Natl Herbarium, MRC 166,POB 37012, Washington, DC 20013 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47097 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47097 USA. [Loladze, Irakli] Bryan Coll Hlth Sci, Bryan Med Ctr, Lincoln, NE 68506 USA. [Polley, H. Wayne] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Ziska, LH (reprint author), USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. EM l.ziska@ars.usda.gov RI Dukes, Jeffrey/C-9765-2009; OI Dukes, Jeffrey/0000-0001-9482-7743; Loladze, Irakli/0000-0001-7615-7803 NR 58 TC 3 Z9 3 U1 25 U2 46 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 EI 1471-2954 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD APR 13 PY 2016 VL 283 IS 1828 AR 20160414 DI 10.1098/rspb.2016.0414 PG 7 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA DL9LU UT WOS:000375965600008 ER PT J AU Walsh, P Behrens, N Chaigneau, FRC McEligot, H Agrawal, K Newman, JW Anderson, M Gershwin, LJ AF Walsh, Paul Behrens, Nicole Chaigneau, Francisco R. Carvallo McEligot, Heather Agrawal, Karan Newman, John W. Anderson, Mark Gershwin, Laurel J. TI A Randomized Placebo Controlled Trial of Ibuprofen for Respiratory Syncytial Virus Infection in a Bovine Model SO PLOS ONE LA English DT Article ID PUBLIC-HEALTH-SERVICE; 20-CARBON FATTY-ACIDS; WHOLE-BLOOD CULTURES; IN-VITRO; ANTIBACTERIAL TREATMENT; RSV BRONCHIOLITIS; CLINICAL-EFFICACY; CYCLOOXYGENASE 2; REYES-SYNDROME; DISEASE AB Background Respiratory syncytial virus (RSV) is the most common cause of bronchiolitis and hospital admission in infants. An analogous disease occurs in cattle and costs US agriculture a billion dollars a year. RSV causes much of its morbidity indirectly via adverse effects of the host response to the virus. RSV is accompanied by elevated prostaglandin E-2 (PGE(2)) which is followed by neutrophil led inflammation in the lung. Ibuprofen is a prototypical nonsteroidal anti-inflammatory drug that decreases PGE2 levels by inhibiting cyclooxygenase. Hypotheses We hypothesized that treatment of RSV with ibuprofen would decrease PGE2 levels, modulate the immune response, decrease clinical illness, and decrease the histopathological lung changes in a bovine model of RSV. We further hypothesized that viral replication would be unaffected. Methods We performed a randomized placebo controlled trial of ibuprofen in 16 outbred Holstein calves that we infected with RSV. We measured clinical scores, cyclooxygenase, lipoxygenase and endocannabinoid products in plasma and mediastinal lymph nodes and interleukin (Il)-4, II-13, II-17 and interferon-gamma in mediastinal lymph nodes. RSV shedding was measured daily and nasal II-6, II-8 and II-17 every other day. The calves were necropsied on Day 10 post inoculation and histology performed. Results One calf in the ibuprofen group required euthanasia on Day 8 of infection for respiratory distress. Clinical scores (p<0.01) and weight gain (p = 0.08) seemed better in the ibuprofen group. Ibuprofen decreased cyclooxygenase, lipoxygenase, and cytochrome P450 products, and increased monoacylglycerols in lung lymph nodes. Ibuprofen modulated the immune response as measured by narrowed range of observed II-13, II-17 and IFN-gamma gene expression in mediastinal lymph nodes. Lung histology was not different between groups, and viral shedding was increased in calves randomized to ibuprofen. Conclusions Ibuprofen decreased PGE(2), modulated the immune response, and improved clinical outcomes. However lung histopathology was not affected and viral shedding was increased. C1 [Walsh, Paul] Sutter Med Ctr Sacramento, Dept Emergency Med, Div Pediat Emergency Med, Sacramento, CA USA. [Behrens, Nicole; McEligot, Heather; Gershwin, Laurel J.] Univ Calif Davis, Dept Pathol Microbiol & Immunol, Sch Vet Med, 1 Shields Ave, Davis, CA 95616 USA. [Chaigneau, Francisco R. Carvallo] Calif Anim Hlth & Food Safety Lab, San Bernardino Branch, 105 W Cent Ave, San Bernardino, CA USA. [Newman, John W.] ARS, Obes & Metab Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Agrawal, Karan; Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Agrawal, Karan; Newman, John W.] Univ Calif Davis, NIH West Coast Metabol Ctr, Davis, CA 95616 USA. [Anderson, Mark] Calif Anim Hlth & Food Safety Lab, 620 W Hlth Sci Dr, Davis, CA USA. RP Walsh, P (reprint author), Sutter Med Ctr Sacramento, Dept Emergency Med, Div Pediat Emergency Med, Sacramento, CA USA. EM yousentwhohome@gmail.com FU National Heart, Lung, and Blood Institute at the National Institutes of Health (NIH) [5K12HL108964-02]; Pediatric Emergency Medicine Research Foundation, Long Beach, California; USDA [5306-51530-019-00D, 2032-5130-022-00D]; West Coast Metabolomics Center Grant [NIH U24 DK097154] FX This study was funded by Award 5K12HL108964-02 from the National Heart, Lung, and Blood Institute at the National Institutes of Health (NIH) and by the Pediatric Emergency Medicine Research Foundation, Long Beach, California. Additional support was provided by the USDA Intramural Projects 5306-51530-019-00D and 2032-5130-022-00D, and the West Coast Metabolomics Center Grant NIH U24 DK097154. The USDA is an equal opportunity provider and employer. NR 63 TC 2 Z9 2 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 13 PY 2016 VL 11 IS 4 AR e0152913 DI 10.1371/journal.pone.0152913 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ3UC UT WOS:000374131200044 PM 27073858 ER PT J AU Lim, GH Shine, MB de Lorenzo, L Yu, KS Cui, WE Navarre, D Hunt, AG Lee, JY Kachroo, A Kachroo, P AF Lim, Gah-Hyun Shine, M. B. de Lorenzo, Laura Yu, Keshun Cui, Weier Navarre, Duroy Hunt, Arthur G. Lee, Jung-Youn Kachroo, Aardra Kachroo, Pradeep TI Plasmodesmata Localizing Proteins Regulate Transport and Signaling during Systemic Acquired Immunity in Plants SO CELL HOST & MICROBE LA English DT Article ID SALICYLIC-ACID; ARABIDOPSIS; RESISTANCE; COMMUNICATION; DEFENSE; LIPIDS AB Systemic acquired resistance (SAR) in plants is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Here, we show that AzA and G3P transport occurs via the symplastic route, which is regulated by channels known as plasmodesmata (PD). In contrast, SA moves via the extracytosolic apoplast compartment. We found that PD localizing proteins (PDLP) 1 and 5 were required for SAR even though PD permeability in pdlp1 and 5 mutants was comparable to or higher than wild-type plants, respectively. Furthermore, PDLP function was required in the recipient cell, suggesting regulatory function in SAR. Interestingly, overexpression of PDLP5 drastically reduced PD permeability, yet also impaired SAR. PDLP1 interacted with AZI1 (lipid transfer-like protein required for AzA-and G3P-induced SAR) and contributed to its intracellular partitioning. Together, these results reveal the transport routes of SAR chemical signals and highlight the regulatory role of PD-localizing proteins in SAR. C1 [Lim, Gah-Hyun; Shine, M. B.; Yu, Keshun; Kachroo, Aardra; Kachroo, Pradeep] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [de Lorenzo, Laura; Hunt, Arthur G.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Navarre, Duroy] Washington State Univ, USDA ARS, Prosser, WA 99350 USA. [Cui, Weier; Lee, Jung-Youn] Univ Delaware, Dept Plant & Soil Sci, Delaware Biotechnol Inst, Newark, DE 19711 USA. RP Kachroo, A; Kachroo, P (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM apkach2@uky.edu; pk62@uky.edu FU National Science Foundation (IOS) [0749731, 1457121, 0954931]; NSF [1355438] FX This work was supported by grants from the National Science Foundation (IOS#0749731, #1457121, #0954931) and a NSF grant under Cooperative Agreement No. 1355438. We thank Andrea Pitzschke for AZI1-MYC transgenic plants, Arabidopsis database for PDLP knockout seeds, Xiangnan Guan for help with isolation of pdlp mutants, John Johnson for help with gas chromatography, Timothy Hoey for genotyping, Wendy Havens and Ludmila Lapchyk for technical help, and Amy Crume for managing the plant growth facility. NR 24 TC 2 Z9 2 U1 8 U2 22 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1931-3128 EI 1934-6069 J9 CELL HOST MICROBE JI Cell Host Microbe PD APR 13 PY 2016 VL 19 IS 4 BP 541 EP 549 DI 10.1016/j.chom.2016.03.006 PG 9 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA DJ3RP UT WOS:000374123600015 PM 27078071 ER PT J AU Alaswad, AA Krishnan, HB AF Alaswad, Alaa A. Krishnan, Hari B. TI Immunological Investigation for the Presence of Lunasin, a Chemopreventive Soybean Peptide, in the Seeds of Diverse Plants SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE cereal; legume; lunasin; soybean ID CORE HISTONE ACETYLATION; BIOACTIVE PEPTIDE; STORAGE PROTEINS; MAMMALIAN-CELLS; GLYCINE-MAX; SOY PROTEIN; CANCER; PRODUCTS; CHROMATIN; BARLEY AB Lunasin, a 44 amino acid soybean bioactive peptide, exhibits anticancer and anti-inflammatory properties. All soybean varieties that have been examined contain lunasin. It has also been reported in a few other plant species including amaranth, black nightshade, wheat, barley, rye, and triticale. Interestingly, detailed searches of transcriptome and DNA sequence databases of cereals failed to identify lunasin-coding sequences, raising questions about the authenticity of lunasin in cereals. To clarify the presence or absence of lunasin in cereals and other plant species, an immunological investigation was conducted utilizing polydonal antibodies raised against the first 20 amino acid N-terminal peptide (SKWQHQQDSCRKQLQGVNLT) and a 15 amino acid C-terminal peptide (CEKHIMEKIQGRGDD) of lunasin. Protein blot analyses revealed the presence of proteins from several plants that reacted against the lunasin N-terminal peptide antibodies. However, the same proteins failed to react against the lunasin C-terminal peptide antibodies. These results demonstrate that peptides identical to soybean lunasin are absent in seeds of diverse plants examined in this study. C1 [Alaswad, Alaa A.; Krishnan, Hari B.] Univ Missouri, Plant Sci Div, Columbia, MO 65211 USA. [Alaswad, Alaa A.] King Abdulaziz Univ, Jeddah 21413, Saudi Arabia. [Krishnan, Hari B.] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. RP Krishnan, HB (reprint author), Univ Missouri, Plant Genet Res Unit, USDA, ARS, 108 Curtis Hall, Columbia, MO 65211 USA. EM Hari.Krishnan@ARS.USDA.GOV FU USDA Agricultural Research Service FX The research was supported by the USDA Agricultural Research Service. Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 42 TC 1 Z9 1 U1 10 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 13 PY 2016 VL 64 IS 14 BP 2901 EP 2909 DI 10.1021/acs.jafc.6b00445 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DJ5UH UT WOS:000374275100012 PM 27015324 ER PT J AU Chen, JL Zheng, Y Qin, L Wang, Y Chen, LF He, YJ Fei, ZJ Lu, G AF Chen, Jingli Zheng, Yi Qin, Li Wang, Yan Chen, Lifei He, Yanjun Fei, Zhangjun Lu, Gang TI Identification of miRNAs and their targets through high-throughput sequencing and degradome analysis in male and female Asparagus officinalis SO BMC PLANT BIOLOGY LA English DT Article DE Asparagus officinalis; miRNAs; High-throughput sequencing; Degradome analysis; Sex determination ID FLORAL ORGAN IDENTITY; FLOWERING TIME; AUXIN RESPONSE; SMALL RNA; ARABIDOPSIS-THALIANA; CONSERVED MICRORNAS; MEDICAGO-TRUNCATULA; WIDE IDENTIFICATION; SEX DETERMINATION; CELL-DIVISION AB Background: MicroRNAs (miRNAs), a class of non-coding small RNAs (sRNAs), regulate various biological processes. Although miRNAs have been identified and characterized in several plant species, miRNAs in Asparagus officinalis have not been reported. As a dioecious plant with homomorphic sex chromosomes, asparagus is regarded as an important model system for studying mechanisms of plant sex determination. Results: Two independent sRNA libraries from male and female asparagus plants were sequenced with Illumina sequencing, thereby generating 4.13 and 5.88 million final clean reads, respectively. Both libraries predominantly contained 24-nt sRNAs, followed by 21-nt sRNAs. Further analysis identified 154 conserved miRNAs, which belong to 26 families, and 39 novel miRNA candidates seemed to be specific to asparagus. Comparative profiling revealed that 63 miRNAs exhibited significant differential expression between male and female plants, which was confirmed by real-time quantitative PCR analysis. Among them, 37 miRNAs were significantly up-regulated in the female library, whereas the others were preferentially expressed in the male library. Furthermore, 40 target mRNAs representing 44 conserved and seven novel miRNAs were identified in asparagus through high-throughput degradome sequencing. Functional annotation showed that these target mRNAs were involved in a wide range of developmental and metabolic processes. Conclusions: We identified a large set of conserved and specific miRNAs and compared their expression levels between male and female asparagus plants. Several asparagus miRNAs, which belong to the miR159, miR167, and miR172 families involved in reproductive organ development, were differentially expressed between male and female plants, as well as during flower development. Consistently, several predicted targets of asparagus miRNAs were associated with floral organ development. These findings suggest the potential roles of miRNAs in sex determination and reproductive developmental processes in asparagus. C1 [Chen, Jingli; Qin, Li; Wang, Yan; Chen, Lifei; He, Yanjun; Lu, Gang] Zhejiang Univ, Dept Hort, Key Lab Hort Plant Growth Dev & Biotechnol, Agr Minist China, Hangzhou 310058, Zhejiang, Peoples R China. [Zheng, Yi; Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA Robert W Holley Ctr Agr & Hlth, Tower Rd, Ithaca, NY 14853 USA. RP Lu, G (reprint author), Zhejiang Univ, Dept Hort, Key Lab Hort Plant Growth Dev & Biotechnol, Agr Minist China, Hangzhou 310058, Zhejiang, Peoples R China. EM glu@zju.edu.cn RI Zheng, Yi/F-6150-2016 OI Zheng, Yi/0000-0002-8042-7770 FU National 12th-Five Year Research Program of China [2011BAD12B04]; Agriculture Public Welfare Scientific Research Project, Ministry of Agriculture of China [201003074-3]; Zhejiang Provincial Key Project [2012C12012-3]; Hangzhou Science and Technology Bureau [20130932H01] FX This work was supported by Key Project of the National 12th-Five Year Research Program of China (Grant No. 2011BAD12B04), Agriculture Public Welfare Scientific Research Project, Ministry of Agriculture of China (Grant No. 201003074-3), Zhejiang Provincial Key Project (Grant No. 2012C12012-3) and Hangzhou Science and Technology Bureau (20130932H01). NR 79 TC 1 Z9 1 U1 1 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD APR 12 PY 2016 VL 16 AR 80 DI 10.1186/s12870-016-0770-z PG 19 WC Plant Sciences SC Plant Sciences GA DI7OM UT WOS:000373690700001 PM 27068118 ER PT J AU Simkin, SM Allen, EB Bowman, WD Clark, CM Belnap, J Brooks, ML Cade, BS Collins, SL Geiser, LH Gilliam, FS Jovan, SE Pardo, LH Schulz, BK Stevens, CJ Suding, KN Throop, HL Waller, DM AF Simkin, Samuel M. Allen, Edith B. Bowman, William D. Clark, Christopher M. Belnap, Jayne Brooks, Matthew L. Cade, Brian S. Collins, Scott L. Geiser, Linda H. Gilliam, Frank S. Jovan, Sarah E. Pardo, Linda H. Schulz, Bethany K. Stevens, Carly J. Suding, Katharine N. Throop, Heather L. Waller, Donald M. TI Conditional vulnerability of plant diversity to atmospheric nitrogen deposition across the United States SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE nitrogen deposition; plant species richness; diversity; soil pH; climate ID SPECIES RICHNESS; NUTRIENT ENRICHMENT; ECOSYSTEM RESPONSE; BIODIVERSITY LOSS; CRITICAL LOADS; VEGETATION; GRASSLANDS; EUTROPHICATION; TERRESTRIAL; COMMUNITIES AB Atmospheric nitrogen (N) deposition has been shown to decrease plant species richness along regional deposition gradients in Europe and in experimental manipulations. However, the general response of species richness to N deposition across different vegetation types, soil conditions, and climates remains largely unknown even though responses may be contingent on these environmental factors. We assessed the effect of N deposition on herbaceous richness for 15,136 forest, woodland, shrubland, and grassland sites across the continental United States, to address how edaphic and climatic conditions altered vulnerability to this stressor. In our dataset, with N deposition ranging from 1 to 19 kg N.ha(-1).y(-1), we found a unimodal relationship; richness increased at low deposition levels and decreased above 8.7 and 13.4 kg N.ha(-1).y(-1) in open and closed-canopy vegetation, respectively. N deposition exceeded critical loads for loss of plant species richness in 24% of 15,136 sites examined nationwide. There were negative relationships between species richness and N deposition in 36% of 44 community gradients. Vulnerability to N deposition was consistently higher in more acidic soils whereas the moderating roles of temperature and precipitation varied across scales. We demonstrate here that negative relationships between N deposition and species richness are common, albeit not universal, and that fine-scale processes can moderate vegetation responses to N deposition. Our results highlight the importance of contingent factors when estimating ecosystem vulnerability to N deposition and suggest that N deposition is affecting species richness in forested and nonforested systems across much of the continental United States. C1 [Simkin, Samuel M.; Bowman, William D.; Suding, Katharine N.] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. [Simkin, Samuel M.; Bowman, William D.; Suding, Katharine N.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Allen, Edith B.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Allen, Edith B.] Univ Calif Riverside, Ctr Conservat Biol, Riverside, CA 92521 USA. [Clark, Christopher M.] US EPA, Natl Ctr Environm Assessment, Washington, DC 20460 USA. [Belnap, Jayne] US Geol Survey, Southwest Biol Sci Ctr, Moab, UT 84532 USA. [Brooks, Matthew L.] US Geol Survey, Western Ecol Res Ctr, Oakhurst, CA 93644 USA. [Cade, Brian S.] US Geol Survey, Ft Collins Sci Ctr, Ft Collins, CO 80226 USA. [Collins, Scott L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Geiser, Linda H.] US Forest Serv, Pacific Northwest Reg Air Resource Management Pro, USDA, Corvallis, OR 97339 USA. [Gilliam, Frank S.] Marshall Univ, Dept Biol Sci, Huntington, WV 25755 USA. [Jovan, Sarah E.] US Forest Serv, Forest Inventory & Anal Program, USDA, Portland, OR 97339 USA. [Pardo, Linda H.] US Forest Serv, No Res Stn, USDA, Burlington, VT 05405 USA. [Schulz, Bethany K.] US Forest Serv, Forest Inventory & Anal Program, USDA, Anchorage, AK 99501 USA. [Stevens, Carly J.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Throop, Heather L.] Arizona State Univ, Sch Earth & Space Explorat, Tempe, AZ 85287 USA. [Throop, Heather L.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Waller, Donald M.] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA. RP Simkin, SM (reprint author), Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA.; Simkin, SM (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. EM samuel.simkin@colorado.edu RI Collins, Scott/P-7742-2014; Throop, Heather/D-6391-2012 OI Collins, Scott/0000-0002-0193-2892; Throop, Heather/0000-0002-7963-4342 FU John Wesley Powell Center for Analysis and Synthesis - USGS; US Environmental Protection Agency [EP-12-H-000491]; Cooperative Ecosystem Studies Units Network (National Park Service Grant) [P13AC00407]; Cooperative Ecosystem Studies Units Network (USGS Grant) [G14AC00028]; USGS FX Vegetation data were shared by the Forest Inventory and Analysis Database (FIADB) Vegetation Indicators Program, the Ecological Society of America VegBank, the Minnesota Biological Survey, the New York, Virginia, and West Virginia Natural Heritage Programs, Robert Peet and the Carolina Vegetation Survey, the US National Park Service Southern Colorado Plateau Network, the University of Wisconsin Plant Ecology Laboratory, Kevin Knutson of the US Geological Survey (USGS), and the coauthors. This paper arose from the "Diversity and Nitrogen Deposition" working group supported by the John Wesley Powell Center for Analysis and Synthesis, funded by the USGS. The US Environmental Protection Agency (Contract EP-12-H-000491) and the Cooperative Ecosystem Studies Units Network (National Park Service Grant P13AC00407 and USGS Grant G14AC00028) provided additional funding. The USGS supports the conclusions of research conducted by their employees, and peer reviews and approves all of their products consistent with USGS Fundamental Science Practices. The views expressed in this manuscript do not necessarily reflect the views or policies of the US Environmental Protection Agency or the USDA Forest Service. Mention of trade names or commercial products does not constitute endorsement or recommendation for use. NR 40 TC 8 Z9 8 U1 37 U2 69 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 12 PY 2016 VL 113 IS 15 BP 4086 EP 4091 DI 10.1073/pnas.1515241113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI8PG UT WOS:000373762400055 PM 27035943 ER PT J AU Wei, W Zhu, WJ Cheng, JS Xie, JT Jiang, DH Li, GQ Chen, WD Fu, YP AF Wei, Wei Zhu, Wenjun Cheng, Jiasen Xie, Jiatao Jiang, Daohong Li, Guoqing Chen, Weidong Fu, Yanping TI Nox Complex signal and MAPK cascade pathway are cross-linked and essential for pathogenicity and conidiation of mycoparasite Coniothyrium minitans SO SCIENTIFIC REPORTS LA English DT Article ID NADPH OXIDASE COMPLEX; CELL-WALL INTEGRITY; PODOSPORA-ANSERINA; SCLEROTINIA-SCLEROTIORUM; ASCOSPORE GERMINATION; ASPERGILLUS-NIDULANS; FUSARIUM-GRAMINEARUM; BOTRYTIS-CINEREA; CLAVICEPS-PURPUREA; SEXUAL DEVELOPMENT AB The NADPH oxidase complex of a sclerotial mycoparasite Coniothyrium minitans, an important biocontrol agent against crop diseases caused by Sclerotinia sclerotiorum, was identified and its functions involved in conidiation and mycoparasitism were studied. Gene knock-out and complementary experiments indicated that CmNox1, but not CmNox2, is necessary for conidiation and parasitism, and its expression could be significantly induced by its host fungus. CmNox1 is regulated by CmRac1-CmNoxR and interacts with CmSlt2, a homolog of Saccharomyces cerevisiae Slt2 encoding cell wall integrity-related MAP kinase. In Delta CmNox1, CmSlt2-GFP fusion protein lost the ability to localize to the cell nucleus accurately. The defect of conidiation in Delta CmRac1 could be partially restored by over-expressing CmSlt2, indicating that CmSlt2 was a downstream regulatory factor of CmNox1 and was involved in conidiation and parasitism. The expressions of mycoparasitism-related genes CmPks1, Cmg1 and CH1 were suppressed in the knock-out mutants of the genes in CmNox1-CmSlt2 signal pathway when cultivated either on PDA. Therefore, our study infers that CmRac1-CmNoxR regulates CmNox1-CmSlt2 pathway in regulating conidiation and pathogenicity of C. minitans. C1 [Wei, Wei; Zhu, Wenjun; Jiang, Daohong; Li, Guoqing] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei Province, Peoples R China. [Wei, Wei; Zhu, Wenjun; Cheng, Jiasen; Xie, Jiatao; Jiang, Daohong; Li, Guoqing; Fu, Yanping] Huazhong Agr Univ, Coll Plant Sci & Technol, Prov Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei Province, Peoples R China. [Chen, Weidong] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. [Wei, Wei] Jianghan Univ, Inst Interdisciplinary Res, Wuhan 430056, Hubei Province, Peoples R China. RP Fu, YP (reprint author), Huazhong Agr Univ, Coll Plant Sci & Technol, Prov Key Lab Plant Pathol Hubei Prov, Wuhan 430070, Hubei Province, Peoples R China. EM yanpingfu@mail.hzau.edu.cn FU National '973' Basic Research Program of China [2012cb114000]; National Natural Science Foundation of China [31371895]; Programme of Introducing Talents of Discipline to Universities in China (the 111 Project) [B14032]; Program for Changjiang Scholars and Innovative Research Team in University of China [IRT1247] FX The research was financially supported by the National '973' Basic Research Program of China (Grant No. 2012cb114000), the National Natural Science Foundation of China (grant 31371895), the Programme of Introducing Talents of Discipline to Universities in China (the 111 Project no. B14032), and the Program for Changjiang Scholars and Innovative Research Team in University of China (IRT1247). NR 68 TC 2 Z9 2 U1 4 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD APR 12 PY 2016 VL 6 AR 24325 DI 10.1038/srep24325 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI8PC UT WOS:000373761900001 PM 27066837 ER PT J AU Talavera-Garcia, E Delgado-Lista, J Garcia-Rios, A Delgado-Casado, N Gomez-Luna, P Gomez-Garduno, A Gomez-Delgado, F Alcala-Diaz, JF Yubero-Serrano, E Marin, C Perez-Caballero, AI Fuentes-Jimenez, FJ Camargo, A Rodriguez-Cantalejo, F Tinahones, FJ Ordovas, JM Perez-Jimenez, F Perez-Martinez, P Lopez-Miranda, J AF Talavera-Garcia, Eva Delgado-Lista, Javier Garcia-Rios, Antonio Delgado-Casado, Nieves Gomez-Luna, Purificacion Gomez-Garduno, Angela Gomez-Delgado, Francisco Alcala-Diaz, Juan F. Yubero-Serrano, Elena Marin, Carmen Perez-Caballero, Ana I. Fuentes-Jimenez, Francisco J. Camargo, Antonio Rodriguez-Cantalejo, Fernando Tinahones, Francisco J. Ordovas, Jose M. Perez-Jimenez, Francisco Perez-Martinez, Pablo Lopez-Miranda, Jose TI Influence of Obesity and Metabolic Disease on Carotid Atherosclerosis in Patients with Coronary Artery Disease (CordioPrev Study) SO PLOS ONE LA English DT Article ID INTIMA-MEDIA THICKNESS; AMERICAN-HEART-ASSOCIATION; CARDIOVASCULAR RISK-FACTORS; NORMAL-WEIGHT; INSULIN-RESISTANCE; ALL-CAUSE; HEALTHY; MORTALITY; EVENTS; INFLAMMATION AB Background Recent data suggest that the presence of associated metabolic abnormalities may be important modifiers of the association of obesity with a poorer prognosis in coronary heart disease. We determined the influence of isolated overweight and obesity on carotid intima media thickness (IMT-CC), and also assessed whether this influence was determined by the presence of metabolic abnormalities. Methods 1002 participants from the CordioPrev study were studied at entry. We determined their metabolic phenotypes and performed carotid ultrasound assessment. We evaluated the influence of obesity, overweight and metabolic phenotypes on the IMT-CC. Results Metabolically sick participants (defined by the presence of two or more metabolic abnormalities) showed a greater IMT-CC than metabolically healthy individuals (p = 4 * 10(-6)). Overweight and normal weight patients who were metabolically healthy showed a lower IMT-CC than the metabolically abnormal groups (all p<0.05). When we evaluated only body weight (without considering metabolic phenotypes), overweight or obese patients did not differ significantly from normal-weight patients in their IMT-CC (p = 0.077). However, obesity was a determinant of IMT-CC when compared to the composite group of normal weight and overweight patients (all not obese). Conclusions In coronary patients, a metabolically abnormal phenotype is associated with a greater IMT-CC, and may be linked to a higher risk of suffering new cardiovascular events. The protection conferred in the IMT-CC by the absence of metabolic abnormality may be blunted by the presence of obesity. C1 [Talavera-Garcia, Eva; Delgado-Lista, Javier; Garcia-Rios, Antonio; Delgado-Casado, Nieves; Gomez-Luna, Purificacion; Gomez-Garduno, Angela; Gomez-Delgado, Francisco; Alcala-Diaz, Juan F.; Yubero-Serrano, Elena; Marin, Carmen; Perez-Caballero, Ana I.; Fuentes-Jimenez, Francisco J.; Camargo, Antonio; Perez-Jimenez, Francisco; Perez-Martinez, Pablo; Lopez-Miranda, Jose] Univ Cordoba, Reina Sofia Univ Hosp, IMIBIC, Lipid & Atherosclerosis Unit, Cordoba, Spain. [Talavera-Garcia, Eva; Delgado-Lista, Javier; Garcia-Rios, Antonio; Delgado-Casado, Nieves; Gomez-Luna, Purificacion; Gomez-Garduno, Angela; Gomez-Delgado, Francisco; Alcala-Diaz, Juan F.; Yubero-Serrano, Elena; Marin, Carmen; Perez-Caballero, Ana I.; Fuentes-Jimenez, Francisco J.; Camargo, Antonio; Tinahones, Francisco J.; Perez-Jimenez, Francisco; Perez-Martinez, Pablo; Lopez-Miranda, Jose] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr CIBEROBN, Madrid, Spain. [Rodriguez-Cantalejo, Fernando] Hosp Univ Reina Sofia, Biochem Lab, Cordoba, Spain. [Tinahones, Francisco J.] Hosp Virgen de la Victoria, Dept Endocrinol, Biomed Res Lab, Malaga, Spain. [Ordovas, Jose M.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Ordovas, Jose M.] CNIC, IMDEA, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. RP Delgado-Lista, J (reprint author), Univ Cordoba, Reina Sofia Univ Hosp, IMIBIC, Lipid & Atherosclerosis Unit, Cordoba, Spain. EM javier.delgado@imibic.org RI Yubero-Serrano, Elena /H-4832-2013; OI Gomez Delgado, Francisco/0000-0002-0216-2084 FU Fundacion Patrimonio Comunal Olivarero; CITOLIVA; CEAS; Junta de Andalucia (Consejeria de Salud); Junta de Andalucia (Consejeria de Agricultura y Pesca); Junta de Andalucia (Consejeria de Innovacion); Junta de Andalucia (Ciencia y Empresa); Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud; Ministerio de Medio Ambiente, Medio Rural y Marino, Gobierno de Espana; Ministerio de Ciencia e Innovacion [AGL2009-122270, FIS PI10/01041, FIS PI13/00023]; Ministerio de Economia y Competitividad [AGL2012/39615]; Consejeria de Salud, Junta de Andalucia [PI0193/09, PI-0252/09, PI-0058/10, PI-0206-2013]; Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo [CVI-7450]; European Commission (NUTRITECH European Integrated Project) [289511]; ISCIII research contract (Programa Rio-Hortega); Fondo Europeo de Desarrollo Regional (FEDER) FX The CORDIOPREV study is supported by the Fundacion Patrimonio Comunal Olivarero. Also received additional funding from CITOLIVA, CEAS, Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), Diputaciones de Jaen y Cordoba, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud and Ministerio de Medio Ambiente, Medio Rural y Marino, Gobierno de Espana. It was also partly supported by research grants from the Ministerio de Ciencia e Innovacion (AGL2009-122270 to JL-M, FIS PI10/01041 to PP-M, FIS PI13/00023 to JD-L); Ministerio de Economia y Competitividad (AGL2012/39615 to JL-M); Consejeria de Salud, Junta de Andalucia (PI0193/09 to JL-M, PI-0252/09 to JD-L, and PI-0058/10 to PP-M, PI-0206-2013 to AG-R); Proyecto de Excelencia, Consejeria de Economia, Innovacion, Ciencia y Empleo (CVI-7450 to JL-M). It was also partly supported by a research grant from the European Commission (NUTRITECH European Integrated Project-289511). Francisco Gomez-Delgado is supported by an ISCIII research contract (Programa Rio-Hortega). Also co-financiated by Fondo Europeo de Desarrollo Regional (FEDER). The CIBEROBN is an initiative of the Instituto de Salud Carlos III, Madrid, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 1 Z9 1 U1 2 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 11 PY 2016 VL 11 IS 4 AR e0153096 DI 10.1371/journal.pone.0153096 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DJ0KH UT WOS:000373891000029 PM 27064675 ER PT J AU Broadhurst, CL Chaney, RL AF Broadhurst, Catherine L. Chaney, Rufus L. TI Growth and Metal Accumulation of an Alyssum murale Nickel Hyperaccumulator Ecotype Co-cropped with Alyssum montanum and Perennial Ryegrass in Serpentine Soil SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Alyssum murale; Lolium perenne; nickel hyperaccumulators; ryegrass; co-cropping; phytoremediation; phytomining ID THLASPI-CAERULESCENS; CONTAMINATED SOIL; SEDUM-ALFREDII; PLANTS; PHYTOEXTRACTION; RHIZOSPHERE; NI; MOBILIZATION; LOCALIZATION; LEAVES AB The genus Alyssum (Brassicaceae) contains Ni hyperaccumulators (50), many of which can achieve 30 g kg(-1) Ni in dry leaf. Some Alyssum hyperaccumulators are viable candidates for commercial Ni phytoremediation and phytomining technologies. It is not known whether these species secrete organic and/or amino acids into the rhizosphere to solubilize Ni, or can make use of such acids within the soil to facilitate uptake. It has been hypothesized that in fields with mixed plant species, mobilization of metals by phytosiderophores secreted by Graminaceae plants could affect Alyssum Ni, Fe, Cu, and Mn uptake. We co-cropped the Ni hyperaccumulator Alyssum murale, non-hyperaccumulator A. montanum and perennial ryegrass in a natural serpentine soil. All treatments had standard inorganic fertilization required for ryegrass growth and one treatment was compost amended. After 4 months A. murale leaves and stems contained 3600 mg kg(-1) Ni which did not differ significantly with co-cropping. Overall Ni and Mn concentrations were significantly higher in A. murale than in A. montanum or L. perenne. Copper was not accumulated by either Alyssum species, but L. perenne accumulated up to 10 mg kg(-1). A. montanum could not compete with either A. murale or ryegrass, and neither Alyssum species survived in the compost-amended soil. Co-cropping with ryegrass reduced Fe and Mn concentrations in A. murale but not to the extent of either increasing Ni uptake or affecting plant nutrition. The hypothesized Alyssum Ni accumulation in response to phytosiderophores secreted by co-cropped grass did not occur. Our data do not support increased mobilization of Mn by a phytosiderophore mechanism either, but the converse: mobilization of Mn by theAlyssum hyperaccumulator species significantly increased Mn levels in L. perenne. Tilling soil to maximize root penetration, adequate inorganic fertilization and appropriate plant densities are more important for developing efficient phytoremediation and phytomining approaches. C1 [Broadhurst, Catherine L.] USDA ARS, Environm Microbiol & Food Safety Lab, Beltsville, MD USA. [Broadhurst, Catherine L.] Univ Maryland, Dept Food Sci & Nutr, College Pk, MD 20742 USA. [Chaney, Rufus L.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD USA. RP Broadhurst, CL (reprint author), USDA ARS, Environm Microbiol & Food Safety Lab, Beltsville, MD USA.; Broadhurst, CL (reprint author), Univ Maryland, Dept Food Sci & Nutr, College Pk, MD 20742 USA. EM leigh.broadhurst@ars.usda.gov NR 53 TC 1 Z9 1 U1 13 U2 23 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD APR 8 PY 2016 VL 7 AR 451 DI 10.3389/fpls.2016.00451 PG 9 WC Plant Sciences SC Plant Sciences GA DI6HT UT WOS:000373600500001 PM 27092164 ER PT J AU Grogan, SM Brown-Guedira, G Haley, SD McMaster, GS Reid, SD Smith, J Byrne, PF AF Grogan, Sarah M. Brown-Guedira, Gina Haley, Scott D. McMaster, Gregory S. Reid, Scott D. Smith, Jared Byrne, Patrick F. TI Allelic Variation in Developmental Genes and Effects on Winter Wheat Heading Date in the US Great Plains SO PLOS ONE LA English DT Article ID TRITICUM-AESTIVUM L.; SOUTHERN AUSTRALIAN WHEAT; HEXAPLOID WHEAT; VERNALIZATION RESPONSE; RHT-1 LOCI; CL WHEAT; REGISTRATION; PHOTOPERIOD; PPD-D1; CULTIVARS AB Heading date in wheat (Triticum aestivum L.) and other small grain cereals is affected by the vernalization and photoperiod pathways. The reduced-height loci also have an effect on growth and development. Heading date, which occurs just prior to anthesis, was evaluated in a population of 299 hard winter wheat entries representative of the U.S. Great Plains region, grown in nine environments during 2011-2012 and 2012-2013. The germplasm was evaluated for candidate genes at vernalization (Vrn-A1, Vrn-B1, and Vrn-D1), photoperiod (Ppd-A1, Ppd-B1 and Ppd-D1), and reduced-height (Rht-B1 and Rht-D1) loci using polymerase chain reaction (PCR) and Kompetitive Allele Specific PCR (KASP) assays. Our objectives were to determine allelic variants known to affect flowering time, assess the effect of allelic variants on heading date, and investigate changes in the geographic and temporal distribution of alleles and haplotypes. Our analyses enhanced understanding of the roles developmental genes have on the timing of heading date in wheat under varying environmental conditions, which could be used by breeding programs to improve breeding strategies under current and future climate scenarios. The significant main effects and two-way interactions between the candidate genes explained an average of 44% of variability in heading date at each environment. Among the loci we evaluated, most of the variation in heading date was explained by Ppd-D1, Ppd-B1, and their interaction. The prevalence of the photoperiod sensitive alleles Ppd-A1b, Ppd-B1b, and Ppd-D1b has gradually decreased in U.S. Great Plains germplasm over the past century. There is also geographic variation for photoperiod sensitive and reduced-height alleles, with germplasm from breeding programs in the northern Great Plains having greater incidences of the photoperiod sensitive alleles and lower incidence of the semi-dwarf alleles than germplasm from breeding programs in the central or southern plains. C1 [Grogan, Sarah M.; Haley, Scott D.; Reid, Scott D.; Byrne, Patrick F.] Colorado State Univ, Soil & Crop Sci, Ft Collins, CO 80523 USA. [Brown-Guedira, Gina; Smith, Jared] USDA ARS, Reg Small Grains Genotyping Lab, Raleigh, NC 27695 USA. [McMaster, Gregory S.] USDA ARS, Agr Syst Res Unit, Raleigh, NC 27695 USA. RP Byrne, PF (reprint author), Colorado State Univ, Soil & Crop Sci, Ft Collins, CO 80523 USA. EM Patrick.Byrne@colostate.edu RI Haley, Scott/C-1228-2013; OI Grogan, Sarah/0000-0001-5918-8480 FU USDA National Institute of Food and Agriculture AFRI grant [2011-68002-30029] FX Funding was provided by the USDA National Institute of Food and Agriculture AFRI grant to the Triticeae-CAP project (2011-68002-30029, http://www.triticeaecap.org). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 71 TC 1 Z9 1 U1 1 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 8 PY 2016 VL 11 IS 4 AR e0152852 DI 10.1371/journal.pone.0152852 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI6JK UT WOS:000373604800013 PM 27058239 ER PT J AU Larson, LR Jennings, V Cloutier, SA AF Larson, Lincoln R. Jennings, Viniece Cloutier, Scott A. TI Public Parks and Wellbeing in Urban Areas of the United States SO PLOS ONE LA English DT Article ID PHYSICAL-ACTIVITY; GREEN SPACE; ECOSYSTEM SERVICES; ENVIRONMENTAL-HEALTH; NATURE EXPERIENCE; MENTAL-HEALTH; LOCAL PARKS; LOS-ANGELES; NEIGHBORHOOD; HAPPINESS AB Sustainable development efforts in urban areas often focus on understanding and managing factors that influence all aspects of health and wellbeing. Research has shown that public parks and green space provide a variety of physical, psychological, and social benefits to urban residents, but few studies have examined the influence of parks on comprehensive measures of subjective wellbeing at the city level. Using 2014 data from 44 U.S. cities, we evaluated the relationship between urban park quantity, quality, and accessibility and aggregate self-reported scores on the Gallup-Healthways Wellbeing Index (WBI), which considers five different domains of wellbeing (e.g., physical, community, social, financial, and purpose). In addition to park-related variables, our best-fitting OLS regression models selected using an information theory approach controlled for a variety of other typical geographic and socio-demographic correlates of wellbeing. Park quantity (measured as the percentage of city area covered by public parks) was among the strongest predictors of overall wellbeing, and the strength of this relationship appeared to be driven by parks' contributions to physical and community wellbeing. Park quality (measured as per capita spending on parks) and accessibility (measured as the overall percentage of a city's population within 1/2 mile of parks) were also positively associated with wellbeing, though these relationships were not significant. Results suggest that expansive park networks are linked to multiple aspects of health and wellbeing in cities and positively impact urban quality of life. C1 [Larson, Lincoln R.] Clemson Univ, Dept Pk Recreat & Tourism Management, Clemson, SC USA. [Jennings, Viniece] US Forest Serv, USDA, Southern Res Stn, Integrating Human & Nat Syst, Athens, GA USA. [Cloutier, Scott A.] Arizona State Univ, Julie Ann Wrigley Global Inst Sustainabil, Tempe, AZ USA. RP Larson, LR (reprint author), Clemson Univ, Dept Pk Recreat & Tourism Management, Clemson, SC USA. EM LRL@clemson.edu FU U.S.D.A. joint venture Forest Service [14-JV-11330144-060] FX U.S.D.A. joint venture Forest Service agreement #14-JV-11330144-060; U.S. Forest Service employee VJ was involved with the study design, data collection and analysis, and preparation of the manuscript. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the Forest Service or USDA. NR 109 TC 1 Z9 1 U1 43 U2 74 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 7 PY 2016 VL 11 IS 4 AR e0153211 DI 10.1371/journal.pone.0153211 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI6KO UT WOS:000373608000102 PM 27054887 ER PT J AU van der Graaf-van Bloois, L Miller, WG Yee, E Gorkiewicz, G Forbes, KJ Zomer, AL Wagenaar, JA Duim, B AF van der Graaf-van Bloois, Linda Miller, William G. Yee, Emma Gorkiewicz, Gregor Forbes, Ken J. Zomer, Aldert L. Wagenaar, Jaap A. Duim, Birgitta TI Campylobacter fetus Subspecies Contain Conserved Type IV Secretion Systems on Multiple Genomic Islands and Plasmids SO PLOS ONE LA English DT Article ID HORIZONTAL GENE-TRANSFER; PATHOGENICITY ISLAND; HELICOBACTER-PYLORI; SERUM-RESISTANCE; PATHOGENESIS; INFECTIONS; VIRULENCE; PROTEINS; DISEASE; HUMANS AB The features contributing to differences in pathogenicity of the Campylobacter fetus subspecies are unknown. Putative factors involved in pathogenesis are located in genomic islands that encode a type IV secretion system (T4SS) and fic domain (filamentation induced by cyclic AMP) proteins, which may disrupt host cell processes. In the genomes of 27 C. fetus strains, three phylogenetically-different T4SS-encoding regions (T4SSs) were identified: one was located in both the chromosome and in extra-chromosomal plasmids; one was located exclusively in the chromosome; and one exclusively in extra-chromosomal plasmids. We observed that C. fetus strains can contain multiple T4SSs and that homologous T4SSs can be present both in chromosomal genomic islands (GI) and on plasmids in the C. fetus strains. The GIs of the chromosomally located T4SS differed mainly by the presence of fic genes, insertion sequence elements and phage-related or hypothetical proteins. Comparative analysis showed that T4SS sequences, inserted in the same locations, were conserved in the studied C. fetus genomes. Using phylogenetic analysis of the T4SSs, it was shown that C. fetus may have acquired the T4SS regions from other Campylobacter species by horizontal gene transfer. The identified T4SSs and fic genes were found in Cff and Cfv strains, although the presence of T4SSs and fic genes were significantly associated with Cfv strains. The T4SSs and fic genes could not be associated with S-layer serotypes or geographical origin of the strains. C1 [van der Graaf-van Bloois, Linda; Zomer, Aldert L.; Wagenaar, Jaap A.; Duim, Birgitta] Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Utrecht, Netherlands. [van der Graaf-van Bloois, Linda; Zomer, Aldert L.; Wagenaar, Jaap A.; Duim, Birgitta] WHO Collaborating Ctr Campylobacter OIE Reference, Utrecht, Netherlands. [Miller, William G.; Yee, Emma] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. [Gorkiewicz, Gregor] Med Univ Graz, Inst Pathol, Graz, Austria. [Forbes, Ken J.] Univ Aberdeen, Sch Med & Dent, Aberdeen, Scotland. [Wagenaar, Jaap A.] Cent Vet Inst Wageningen UR, Lelystad, Netherlands. RP Duim, B (reprint author), Univ Utrecht, Fac Vet Med, Dept Infect Dis & Immunol, Utrecht, Netherlands.; Duim, B (reprint author), WHO Collaborating Ctr Campylobacter OIE Reference, Utrecht, Netherlands. EM B.Duim@uu.nl OI Zomer, Aldert/0000-0002-0758-5190; Gorkiewicz, Gregor/0000-0003-1149-4782 NR 33 TC 2 Z9 2 U1 3 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 6 PY 2016 VL 11 IS 4 AR e0152832 DI 10.1371/journal.pone.0152832 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI6IX UT WOS:000373603500059 ER PT J AU Wallace, ZP Kennedy, PL Squires, JR Oakleaf, RJ Olson, LE Dugger, KM AF Wallace, Zachary P. Kennedy, Patricia L. Squires, John R. Oakleaf, Robert J. Olson, Lucretia E. Dugger, Katie M. TI Re-Occupancy of Breeding Territories by Ferruginous Hawks in Wyoming: Relationships to Environmental and Anthropogenic Factors SO PLOS ONE LA English DT Article ID ENERGY DEVELOPMENT; SWAINSONS HAWKS; HABITAT QUALITY; SPOTTED OWLS; BARRED OWLS; POPULATIONS; SELECTION; RAPTORS; USA; DISTURBANCE AB Grassland and shrubland birds are declining globally due in part to anthropogenic habitat modification. Because population performance of these species is also influenced by non-anthropogenic factors, it is important to incorporate all relevant ecological drivers into demographic models. We used design-based sampling and occupancy models to test relationships of environmental factors that influence raptor demographics with re-occupancy of breeding territories by ferruginous hawks (Buteo regalis) across Wyoming, USA, 2011-2013. We also tested correlations of territory re-occupancy with oil and gas infrastructure-a leading cause of habitat modification throughout the range of this species of conservation concern. Probability of re-occupancy was not related to any covariates we investigated in 2011, had a strong negative relationship with cover of sagebrush (Artemisia spp.) in 2012, was slightly higher for territories with artificial platforms than other nest substrates in 2013, and had a positive relationship with abundance of ground squirrels (Urocitellus spp.) that was strong in 2012 and weak in 2013. Associations with roads were weak and varied by year, road-type, and scale: in 2012, re-occupancy probability had a weak positive correlation with density of roads not associated with oil and gas fields at the territory-scale; however, in 2013 re-occupancy had a very weak negative correlation with density of oil and gas field roads near nest sites (<= 500 m). Although our results indicate re-occupancy of breeding territories by ferruginous hawks was compatible with densities of anthropogenic infrastructure in our study area, the lack of relationships between oil and gas well density and territory re-occupancy may have occurred because pre-treatment data were unavailable. We used probabilistic sampling at a broad spatial extent, methods to account for imperfect detection, and conducted extensive prey sampling; nonetheless, future research using before-after-control-impact designs is needed to fully assess impacts of oil and gas development on ferruginous hawks. C1 [Wallace, Zachary P.; Kennedy, Patricia L.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Wallace, Zachary P.; Kennedy, Patricia L.] Oregon State Univ, Eastern Oregon Agr & Nat Resource Program, Union, OR USA. [Squires, John R.; Olson, Lucretia E.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Oakleaf, Robert J.] Wyoming Game & Fish Dept, Lander, WY USA. [Dugger, Katie M.] Oregon State Univ, Dept Fisheries & Wildlife, US Geol Survey, Oregon Cooperat Fish & Wildlife Res Unit, Corvallis, OR 97331 USA. [Wallace, Zachary P.] Eagle Environm Inc, Taos, NM USA. RP Wallace, ZP (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA.; Wallace, ZP (reprint author), Oregon State Univ, Eastern Oregon Agr & Nat Resource Program, Union, OR USA.; Wallace, ZP (reprint author), Eagle Environm Inc, Taos, NM USA. EM zach@eagleenvironmental.net FU U.S. Forest Service Rocky Mountain Research Station; Wyoming Game and Fish Department; Bureau of Land Management; Wyoming Wildlife Heritage Foundation; Wyoming Governor's Office; PacifiCorp; Pathfinder Renewable Wind Energy; Oregon State University Department of Fisheries and Wildlife; Eastern Oregon Agriculture & Natural Resource Program FX The U.S. Forest Service Rocky Mountain Research Station, Wyoming Game and Fish Department, and Bureau of Land Management provided funding and assisted with study design and data collection. Additional funding was provided by the Wyoming Wildlife Heritage Foundation, Wyoming Governor's Office, PacifiCorp, and Pathfinder Renewable Wind Energy, none of whom were involved in research design, collection and analysis of data, decision to publish, or preparation of the manuscript.; We thank the U.S. Forest Service Rocky Mountain Research Station, Wyoming Game and Fish Department, Oregon State University Department of Fisheries and Wildlife, and Eastern Oregon Agriculture & Natural Resource Program for providing field and administrative support. We thank Ricardo Mata-Gonzalez for advice on study design; pilots Bobby Laird, Dave Stinson, Neil Cadwell, and Ken Overfield; field technicians Justin Broderick, Raymond Buchheit, Justin Caraway, Joseph Ceradini, David Chrismon, Nick Dobric, Andrew Greenawalt, Tia Hunter, Allyson Larned, Rod Lawrence, Jon Lipke, Peter Mahoney, Ryan Mong, Brennan Price, Mary Raikes, Sam Sellers, Edward Whitehead, Matthew Wilson, and Robert Wiltzen; and all private land owners who granted access for field work. Review by David Weins and two anonymous referees greatly improved the manuscript. The use of trade names or products does not constitute endorsement by the U.S. Government. NR 81 TC 1 Z9 1 U1 4 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 6 PY 2016 VL 11 IS 4 AR e0152977 DI 10.1371/journal.pone.0152977 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DI6IX UT WOS:000373603500075 PM 27049324 ER PT J AU Berkessa, SC Clarke, ZJF Fotie, J Bohle, DS Grimm, CC AF Berkessa, Sololiya C. Clarke, Zachary J. F. Fotie, Jean Bohle, D. Scott Grimm, Casey C. TI Silver(I)-mediated regioselective oxidative cross-coupling of phenol and aniline derivatives resulting in 2 '-aminobiphenyl-2-ols SO TETRAHEDRON LETTERS LA English DT Article DE Dehydrogenative cross-coupling; Silver(I) promoted reaction; Aniline and phenol derivatives; 2 '-Aminobiphenyl-2-ols ID ARYL BOND FORMATION; C-H ACTIVATION; 2-COMPONENT MOLECULAR-CRYSTAL; UNSYMMETRICAL BIPHENOLS; JADOMYCIN DERIVATIVES; CHLOROPEPTIN-I; 2-NAPHTHYLAMINE; 2-NAPHTHOL; REACTIVITY; FUNCTIONALIZATION AB A broad and efficient regioselective aerobic oxidative cross-coupling of phenols and aniline derivatives in the presence of AgNO3 and H2O2 resulting in 2'-aminobiphenyl-2-ols has been developed. This reaction is selective toward the creation of a new C-C bond at the ortho position to both amine and hydroxyl functional groups in the respective starting materials. Although oxidative cross-coupling of phenols and anilines resulting in 2'-aminobiphenyl-2-ol derivatives have been reported, these reactions have been mainly limited to 2-naphthol and 1-naphthol. This is one of the rare reports in which this type of cross-coupling reaction is expanded to simple phenol derivatives. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Berkessa, Sololiya C.; Clarke, Zachary J. F.; Fotie, Jean] SE Louisiana Univ, Dept Chem & Phys, SLU 10878, Hammond, LA 70402 USA. [Bohle, D. Scott] McGill Univ, Dept Chem, 801 Sherbrooke St W, Montreal, PQ H3A 0B8, Canada. [Grimm, Casey C.] USDA, Food Proc & Sensory Qual Res, 1100 Robert E Lee Blvd,Bldg 001 SRRC, New Orleans, LA 70124 USA. RP Fotie, J (reprint author), SE Louisiana Univ, Dept Chem & Phys, SLU 10878, Hammond, LA 70402 USA. EM jean.fotie@selu.edu FU National Science Foundation [MRI-1337372]; Louisiana Board of Regents Support Fund: Supervised Undergraduate Research Experiences [LEQSF-EPS(2015)-SURE-152]; NSERC; CRC FX J.F. acknowledges support from the National Science Foundation under grant # MRI-1337372, and the Louisiana Board of Regents Support Fund: Supervised Undergraduate Research Experiences (LEQSF-EPS(2015)-SURE-152). D.S.B. gratefully acknowledges support from NSERC and the CRC. NR 47 TC 0 Z9 0 U1 9 U2 21 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0040-4039 J9 TETRAHEDRON LETT JI Tetrahedron Lett. PD APR 6 PY 2016 VL 57 IS 14 BP 1613 EP 1618 DI 10.1016/j.tetlet.2016.02.111 PG 6 WC Chemistry, Organic SC Chemistry GA DH7BD UT WOS:000372945300023 ER PT J AU Dhar, S Kumar, A Zhang, LF Rimando, AM Lage, JM Lewin, JR Atfi, A Zhang, X Levenson, AS AF Dhar, Swati Kumar, Avinash Zhang, Liangfen Rimando, Agnes M. Lage, Janice M. Lewin, Jack R. Atfi, Azeddine Zhang, Xu Levenson, Anait S. TI Dietary pterostilbene is a novel MTA1-targeted chemopreventive and therapeutic agent in prostate cancer SO ONCOTARGET LA English DT Article DE pterostilbene; prostate cancer; chemoprevention; therapy; MTA1 ID METASTASIS-ASSOCIATED PROTEIN-1; MTA1 MESSENGER-RNA; TUMOR-SUPPRESSOR; AFRICAN-AMERICAN; GENE-EXPRESSION; CELL-CYCLE; C-MYC; RESVERATROL; APOPTOSIS; COMPLEX AB Overexpression of the epigenetic modifier metastasis-associated protein 1 (MTA1) is associated with aggressive human prostate cancer. The purpose of this study was to determine MTA1-targeted chemopreventive and therapeutic efficacy of pterostilbene, a natural potent analog of resveratrol, in pre-clinical models of prostate cancer. Here, we show that high levels of MTA1 expression in Pten-loss prostate cooperate with key oncogenes, including c-Myc and Akt among others, to promote prostate cancer progression. Loss-of-function studies using human prostate cancer cells indicated direct involvement of MTA1 in inducing inflammation and epithelial-to- mesenchymal transition. Importantly, pharmacological inhibition of MTA1 by pterostilbene resulted in decreased proliferation and angiogenesis and increased apoptosis. This restrained prostatic intraepithelial neoplasia (PIN) formation in prostate-specific Pten heterozygous mice and reduced tumor development and progression in prostate-specific Pten-null mice. Our findings highlight MTA1 as a key upstream regulator of prostate tumorigenesis and cancer progression. More significantly, it offers pre-clinical proof for pterostilbene as a promising lead natural agent for MTA1-targeted chemopreventive and therapeutic strategy to curb prostate cancer. C1 [Dhar, Swati; Kumar, Avinash; Zhang, Liangfen; Atfi, Azeddine; Levenson, Anait S.] Univ Mississippi, Med Ctr, Inst Canc, Jackson, MS 39216 USA. [Zhang, Liangfen; Lage, Janice M.; Lewin, Jack R.; Levenson, Anait S.] Univ Mississippi, Med Ctr, Dept Pathol, Jackson, MS 39216 USA. [Rimando, Agnes M.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS USA. [Atfi, Azeddine] Univ Mississippi, Med Ctr, Dept Biochem, Jackson, MS 39216 USA. [Zhang, Xu] Univ Mississippi, Med Ctr, Ctr Biostat & Bioinformat, Jackson, MS 39216 USA. [Levenson, Anait S.] Long Isl Univ, Arnold & Marie Schwartz Coll Pharm & Hlth Sci, Brooklyn, NY USA. RP Levenson, AS (reprint author), Univ Mississippi, Med Ctr, Inst Canc, Jackson, MS 39216 USA.; Levenson, AS (reprint author), Univ Mississippi, Med Ctr, Dept Pathol, Jackson, MS 39216 USA.; Levenson, AS (reprint author), Long Isl Univ, Arnold & Marie Schwartz Coll Pharm & Hlth Sci, Brooklyn, NY USA. EM alevenson@umc.edu FU Department of Defense Prostate Cancer Research Program [W81XWH-13-1-0370] FX This work was supported in part by the Department of Defense Prostate Cancer Research Program under Award # W81XWH-13-1-0370 to AS Levenson. Views and opinions of, and endorsements by the author(s) do not reflect those of the US Army of the Department of Defense. NR 64 TC 2 Z9 2 U1 0 U2 0 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1949-2553 J9 ONCOTARGET JI Oncotarget PD APR 5 PY 2016 VL 7 IS 14 BP 18469 EP 18484 PG 16 WC Oncology; Cell Biology SC Oncology; Cell Biology GA DL5TE UT WOS:000375699000093 PM 26943043 ER PT J AU Mao, XF Qi, YH Huang, JW Liu, JX Chen, GY Na, X Wang, M Qian, YZ AF Mao, Xuefei Qi, Yuehan Huang, Junwei Liu, Jixin Chen, Guoying Na, Xing Wang, Min Qian, Yongzhong TI Ambient-Temperature Trap/Release of Arsenic by Dielectric Barrier Discharge and Its Application to Ultratrace Arsenic Determination in Surface Water Followed by Atomic Fluorescence Spectrometry SO ANALYTICAL CHEMISTRY LA English DT Article ID LIQUID-LIQUID MICROEXTRACTION; IN-SITU PRECONCENTRATION; SLOTTED QUARTZ TUBE; ABSORPTION-SPECTROMETRY; VAPOR GENERATION; HYDRIDE GENERATION; DRINKING-WATER; EMISSION-SPECTROMETRY; MASS-SPECTROMETRY; SAMPLES AB A novel dielectric barrier discharge reactor (DBDR) was utilized to trap/release arsenic coupled to hydride generation atomic fluorescence spectrometry (HG-AFS). On the DBD principle, the precise and accurate control of trap/release procedures was fulfilled at ambient temperature, and an analytical method was established for ultratrace arsenic in real samples. Moreover, the effects of voltage, oxygen, hydrogen, and water vapor on trapping and releasing arsenic by DBDR were investigated. For trapping, arsenic could be completely trapped in DBDR at 40 mL/min of O-2 input mixed with 600 mL/min Ar carrier gas and 9.2 kV discharge potential; prior to release, the Ar carrier gas input should be changed from the upstream gas liquid separator (GLS) to the downstream GLS and kept for 180 s to eliminate possible water vapor interference; for arsenic release, O-2 was replaced by 200 mL/min H-2 and discharge potential was adjusted to 9.5 kV. Under optimized conditions, arsenic could be detected as low as 1.0 ng/L with an 8-fold enrichment factor; the linearity of calibration reached R-2 > 0.995 in the 0.05 mu g/L-5 mu g/L range. The mean spiked recoveries for tap, river, lake, and seawater samples were 98% to 103%; and the measured values of the CRMs including GSB-Z50004-200431, GBW08605, and GBW(E)080390 were in good agreement with the certified values. These findings proved the feasibility of DBDR as an arsenic preconcentration tool for atomic spectrometric instrumentation and arsenic recycling in industrial waste gas discharge. C1 [Mao, Xuefei; Qi, Yuehan; Liu, Jixin; Na, Xing; Wang, Min; Qian, Yongzhong] Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China. [Mao, Xuefei; Qi, Yuehan; Liu, Jixin; Na, Xing; Wang, Min; Qian, Yongzhong] Minist Agr, Key Lab Agrofood Safety & Qual, Beijing 100081, Peoples R China. [Huang, Junwei; Liu, Jixin] Beijing Titan Instruments Co Ltd, Beijing 100015, Peoples R China. [Chen, Guoying] ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Liu, JX; Wang, M (reprint author), Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China.; Liu, JX; Wang, M (reprint author), Minist Agr, Key Lab Agrofood Safety & Qual, Beijing 100081, Peoples R China.; Liu, JX (reprint author), Beijing Titan Instruments Co Ltd, Beijing 100015, Peoples R China. EM ljx2117@gmail.com; wangmincaas@126.com FU National Natural Science Fund of China [31571924, 31301491]; Special Project for National Key Scientific Instruments and Equipment Development of China [2011YQ140149] FX We acknowledge the financial support from the National Natural Science Fund of China (Grant No. 31571924), National Natural Science Fund of China (Grant No. 31301491), and Special Project for National Key Scientific Instruments and Equipment Development of China (Grant No. 2011YQ140149). NR 31 TC 4 Z9 4 U1 18 U2 31 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD APR 5 PY 2016 VL 88 IS 7 BP 4147 EP 4152 DI 10.1021/acs.analchem.6b00506 PG 6 WC Chemistry, Analytical SC Chemistry GA DI7CI UT WOS:000373656300096 PM 26976077 ER PT J AU Finkenstadt, VL Felker, FC Fanta, GF Kenar, JA Selling, GW Hornback, KJ Fisk, DL AF Finkenstadt, Victoria L. Felker, Frederick C. Fanta, George F. Kenar, James A. Selling, Gordon W. Hornback, Kathy J. Fisk, Donald L. TI Extruded foams prepared from high amylose starch with sodium stearate to form amylose inclusion complexes SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE biodegradable; biomaterials; extrusion; foams; microscopy ID SCREW EXTRUSION-COOKING; FATTY-ACID COMPLEXES; STEAM JET COOKING; POLY(VINYL ALCOHOL); PHYSICAL-PROPERTIES; LIPID COMPLEXES; STRUCTURAL CHARACTERISTICS; MANIOC STARCH; POTATO STARCH; WHEAT-FLOUR AB Extruded starch foams were prepared from high amylose corn starch with and without sodium stearate and poly(vinyl alcohol) (PVOH) to determine how the formation of amylose-sodium stearate inclusion complexes and PVOH addition would affect foam properties. X-ray diffraction and Differential Scanning Calorimetry (DSC) showed that amylose-sodium stearate inclusion complexes were formed by low temperature extrusion and did not dissociate during foam formation by a second extrusion at higher temperatures. In the absence of PVOH, water absorption, and foam shrinkage at 95% RH were decreased because of the hydrophobicity of the complex. PVOH addition increased both the expansion ratio and the shrinkage of the foam, although shrinkage at 95% RH was still less than that observed with uncomplexed amylose. The structural integrity and some tensile properties of stearate-containing foams were improved by PVOH addition. These results provide the manufacturer of biodegradable starch foams with an inexpensive method for tailoring foam properties for specific end-use applications. (C) 2015 Wiley Periodicals, Inc. C1 [Finkenstadt, Victoria L.; Felker, Frederick C.; Fanta, George F.; Selling, Gordon W.; Hornback, Kathy J.] USDA ARS, Natl Ctr Agr Utilizat Res, Plant Polymer Res Unit, Peoria, IL 61604 USA. [Kenar, James A.] USDA ARS, Natl Ctr Agr Utilizat Res, Funct Foods Res Unit, Peoria, IL 61604 USA. [Fisk, Donald L.] Rolla Natl Biopolymers LLC, Rolla, MO 65401 USA. RP Felker, FC (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Plant Polymer Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM Frederick.Felker@ars.usda.gov NR 48 TC 0 Z9 0 U1 5 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD APR 5 PY 2016 VL 133 IS 13 AR 43251 DI 10.1002/APP.43251 PG 10 WC Polymer Science SC Polymer Science GA DA6LA UT WOS:000367914700028 ER PT J AU Portman, ZM Griswold, T Pitts, JP AF Portman, Zachary M. Griswold, Terry Pitts, James P. TI Association of the female of Perdita (Xeromacrotera) cephalotes (Cresson), and a replacement name for Perdita bohartorum Parker (Hymenoptera: Andrenidae) SO ZOOTAXA LA English DT Article DE Perdita boharti; bee; synonymy; sex association; distribution AB The monotypic Perdita subgenus Xeromacrotera Timberlake is currently known only from the male sex. Here, the female of Perdita (Xeromacrotera) cephalotes (Cresson, 1878) is associated, resulting in two new junior synonyms of P. (X.) cephalotes: Perdita (Procockerellia) excellens Timberlake, 1958, new synonym and Perdita (Cockerellia) autumnalis Timberlake, 1977, new synonym. The true number of maxillary palpi are reported, and updated subgeneric and species diagnoses are provided. In addition, Perdita boharti Portman & Griswold, new name, is designated as a replacement name for the primary junior homonym P. bohartorum Parker, 1983 (not P. bohartorum Timberlake, 1956) and is assigned to the related subgenus Cockerellia Ashmead. C1 [Portman, Zachary M.] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. [Griswold, Terry] Utah State Univ, USDA ARS, Pollinating Insects Res Unit, 5310 Old Main Hill, Logan, UT 84322 USA. [Pitts, James P.] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. RP Portman, ZM (reprint author), Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA.; Griswold, T (reprint author), Utah State Univ, USDA ARS, Pollinating Insects Res Unit, 5310 Old Main Hill, Logan, UT 84322 USA.; Pitts, JP (reprint author), Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. EM zportman@gmail.com; terry.griswold@ars.usda.gov; jpitts@biology.usu.edu FU National Science Foundation [DGE-1147384]; Utah State University Graduate Enhancement Award; USU Ecology Center Research Award; Utah Agricultural Experiment Station, Utah State University FX We would like to thank Jack Neff for helpful comments on the manuscript as well as Bob Zuparko (CAS) and Jason Weintraub (ANSP) for providing access to types, B. Rozick for databasing, measuring, and mapping specimens, S. Burrows and E. Sadler (USU Department of Biology) for assistance with figures, and H. Ikerd (BBSL) for assistance with databasing and figures. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under grant number DGE-1147384 to ZP. Funding was also provided by a Utah State University Graduate Enhancement Award and a USU Ecology Center Research Award. This research was supported by the Utah Agricultural Experiment Station, Utah State University, and approved as journal paper number 8845. NR 14 TC 1 Z9 1 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD APR 4 PY 2016 VL 4097 IS 4 BP 567 EP 574 PG 8 WC Zoology SC Zoology GA DK9PD UT WOS:000375262400008 PM 27394567 ER PT J AU Liu, LF Hsia, MM Dama, M Vogel, J Pauly, M AF Liu, Lifeng Hsia, Mon Mandy Dama, Murali Vogel, John Pauly, Markus TI A Xyloglucan Backbone 6-O-Acetyltransferase from Brachypodium distachyon Modulates Xyloglucan Xylosylation SO MOLECULAR PLANT LA English DT Letter ID O-ACETYLATION; ARABIDOPSIS; BIOSYNTHESIS; PROTEINS; GENE C1 [Liu, Lifeng; Dama, Murali; Vogel, John; Pauly, Markus] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Hsia, Mon Mandy; Vogel, John] USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. [Vogel, John] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. RP Pauly, M (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM mpauly69@berkeley.edu RI Pauly, Markus/B-5895-2008; OI Pauly, Markus/0000-0002-3116-2198; Vogel, John/0000-0003-1786-2689 NR 10 TC 2 Z9 2 U1 0 U2 6 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1674-2052 EI 1752-9867 J9 MOL PLANT JI Mol. Plant. PD APR 4 PY 2016 VL 9 IS 4 BP 615 EP 617 DI 10.1016/j.molp.2015.11.004 PG 3 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DI8HS UT WOS:000373742700013 PM 26589447 ER PT J AU Azzi, A Meydani, SN Meydani, M Zingg, JM AF Azzi, Angelo Meydani, Simin Nikbin Meydani, Mohsen Zingg, Jean Marc TI The rise, the fall and the renaissance of vitamin E SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article DE Vitamin E; Tocopherols; Non-antioxidant functions; Polyphenols; Signal transduction; Gene expression ID PROTEIN-KINASE-C; ALPHA-TOCOPHERYL-PHOSPHATE; ENDOTHELIAL GROWTH-FACTOR; SCAVENGER RECEPTOR EXPRESSION; PREVENTION TRIAL SELECT; HUMAN THP-1 MACROPHAGES; NEUTROPHIL-SUPEROXIDE GENERATION; DIFFERENTIAL GENE-EXPRESSION; RANDOMIZED CONTROLLED-TRIAL; MURINE T-LYMPHOCYTES AB This review deals with the expectations of vitamin E ability of preventing or curing, as a potent antioxidant, alleged oxidative stress based ailments including cardiovascular disease, cancer, neurodegenerative diseases, cataracts, macular degeneration and more. The results obtained with clinical intervention studies have highly restricted the range of effectiveness of this vitamin. At the same time, new non-antioxidant mechanisms have been proposed. The new functions of vitamin E have been shown to affect cell signal transduction and gene expression, both in vitro and in vivo. Phosphorylation of vitamin E, which takes place in vivo, results in a molecule provided with functions that are in part stronger and in part different from those of the non-phosphorylate compound. The in vivo documented functions of vitamin E preventing the vitamin E deficiency ataxia (AVED), slowing down the progression of non-alcoholic steato-hepatitis (NASH), decreasing inflammation and potentiating the immune response are apparently based on these new molecular mechanisms. It should be stressed however that vitamin E, when present at higher concentrations in the body, should exert antioxidant properties to the extent that its chromanol ring is unprotected or un-esterified. (C) 2015 Elsevier Inc. All rights reserved. C1 [Azzi, Angelo; Meydani, Simin Nikbin; Meydani, Mohsen; Zingg, Jean Marc] Tufts Univ, JM USDA HNRCA, Vasc Biol Lab, Off 622,711 Washington St, Boston, MA 02111 USA. RP Azzi, A (reprint author), Tufts Univ, JM USDA HNRCA, Vasc Biol Lab, Off 622,711 Washington St, Boston, MA 02111 USA. EM angelo.azzi@tufts.edu FU US Department of Agriculture [58-1950-0-014] FX This study was supported by US Department of Agriculture, Contract Agreement No. 58-1950-0-014. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 135 TC 2 Z9 2 U1 4 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0003-9861 EI 1096-0384 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD APR 2 PY 2016 VL 595 SI SI BP 100 EP 108 DI 10.1016/j.abb.2015.11.010 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DK1WF UT WOS:000374705000021 PM 27095224 ER PT J AU Englander, AC Douds, DD Mallory, EB AF Englander, Aaron C. Douds, David D., Jr. Mallory, Ellen B. TI On-farm produced microbial soil inoculant effects on bread wheat (Triticum aestivum) production SO BIOLOGICAL AGRICULTURE & HORTICULTURE LA English DT Article DE soil inoculants; mycorrhizae; indigenous microorganism; wheat; on-farm produced inoculants ID ARBUSCULAR MYCORRHIZAL FUNGI; DROUGHT STRESS; GROWTH; FIELD; HYPHAE; ESTABLISHMENT; COLONIZATION; DEPENDENCY; FERTILITY; PHOSPHATE AB The use of microbial soil inoculants in agriculture is of increasing interest among growers and scientists. This study aimed to determine the effects of a commercially available arbuscular mycorrhizal fungus (AMF) inoculant, an on-farm produced AMF inoculant, and an on-farm produced indigenous microbial inoculant on bread wheat (Triticum aestivum L.) AMF colonization, biomass accumulation, nutrient uptake and recovery, and grain yield and protein in a containerized greenhouse experiment and two years of field trials conducted in Maine, USA. The inoculants were compared with respective controls (sterilized inoculants) and the levels of N, P and K were equalized across treatments. Both AMF inoculants enhanced AMF colonization in a greenhouse potting mix containing unsterilized field soil, but only the on-farm produced AMF increased aboveground biomass and phosphorus (P) uptake and recovery. No differences were observed among inoculants and their relative controls in the field. However, the indigenous microbial inoculant (IMO+) increased wheat aboveground biomass, nutrient uptake, nutrient recovery and grain yield when compared with the mycorrhizal inoculants, but no increases were observed when contrasted against its control (IMO-). Wheat growth enhancements from IMO were likely due to nutrient supply from the compost-based material in which it was produced. Results demonstrate that a mycorrhizal inoculant produced on-farm can increase wheat AMF colonization, aboveground biomass, and P uptake and recovery in a containerized setting. Otherwise differences between inoculants and their relative controls were limited, perhaps due to competition from the native soil microbial community, thus inoculation of field plots cannot be recommended for wheat. C1 [Englander, Aaron C.; Mallory, Ellen B.] Univ Maine, Sch Food & Agr, 495 Coll Ave, Orono, ME 04473 USA. [Douds, David D., Jr.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Englander, AC (reprint author), Univ Maine, Sch Food & Agr, 495 Coll Ave, Orono, ME 04473 USA. EM aaron.englander@gmail.com FU USDA National Institute of Food and Agriculture (NIFA) Sustainable Agriculture Research and Education Graduate Student Grant Program [GNE11-16]; Organic Agriculture Research and Extension Initiative [2009-51300-05594]; Hatch Grant [ME08001-1] FX The authors would like to thank Tom Molloy and the MAFES Analytical Laboratory for their technical assistance. This work is based on research supported by the USDA National Institute of Food and Agriculture (NIFA) Sustainable Agriculture Research and Education Graduate Student Grant Program [grant number GNE11-16], "Farm-Grown Microbial Soil Inoculants: Effects on Bread Wheat Yield and Quality," Organic Agriculture Research and Extension Initiative [grant number 2009-51300-05594], "Enhancing Farmers' Capacity to Produce High Quality Organic Bread Wheat," and Hatch Grant number [ME08001-1]. NR 43 TC 0 Z9 0 U1 5 U2 17 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0144-8765 EI 2165-0616 J9 BIOL AGRIC HORTIC JI Biol. Agric. Hortic. PD APR 2 PY 2016 VL 32 IS 2 BP 85 EP 97 DI 10.1080/01448765.2015.1056229 PG 13 WC Agronomy; Horticulture SC Agriculture GA DI1VX UT WOS:000373285800002 ER PT J AU Prasifka, JR Bazzalo, ME AF Prasifka, Jarrad R. Eugenia Bazzalo, Maria TI Susceptibility of sunflower inbreds to Melanagromyza minimoides in Argentina and potential association with plant resistance traits SO INTERNATIONAL JOURNAL OF PEST MANAGEMENT LA English DT Article DE glandular trichomes; host plant resistance; pericarp; girasol; sesquiterpene lactones; Mosquita del capitulo ID HOMOEOSOMA-ELECTELLUM; DIPTERA; AGROMYZIDAE; MOTH; LEPIDOPTERA; ASTERACEAE; PYRALIDAE; MECHANISM; BIOLOGY; LARVAE AB Seed-feeding by larvae of the mosquito head fly, Melanagromyza minimoides, can substantially reduce yields of late-planted sunflower in South America and its management with insecticides or early-planting has other undesirable costs. Evaluation of inbred lines over three seasons indicated differences in the emergence of adult flies, particularly between restorer (male) and maintainer (female) lines used for hybrid seed production; when fly emergence (approximate to damage) was adjusted for differences in head size (i.e. to flies/100 cm(2)), restorer lines averaged 3-12times more damage than maintainer lines. Fly emergence in each of two seasons was tested for correlations with three sunflower traits (glandular trichome number, pericarp strength, and sesquiterpene lactone content) believed to provide host plant resistance to other insects. In one of two years, fly emergence was negatively correlated with the relative amount of sesquiterpene lactones in florets of the tested lines. Also, among maintainer lines, severity of infestation was much greater in multi-headed inbreds compared to single-headed lines. Additional research is needed to establish the cause(s) of reduced susceptibility of inbreds and whether parental lines influence M. minimoides damage to hybrids. C1 [Prasifka, Jarrad R.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. [Eugenia Bazzalo, Maria] Advanta Semillas SAIC, Balcarce, Argentina. RP Prasifka, JR (reprint author), USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. EM jarrad.prasifka@ars.usda.gov NR 26 TC 0 Z9 1 U1 0 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0967-0874 EI 1366-5863 J9 INT J PEST MANAGE JI Int. J. Pest Manage. PD APR 2 PY 2016 VL 62 IS 2 BP 105 EP 110 DI 10.1080/09670874.2015.1115912 PG 6 WC Entomology SC Entomology GA DG9ST UT WOS:000372424800002 ER PT J AU Ho, T Quito-Avila, D Keller, KE Postman, JD Martin, RR Tzanetakis, IE AF Ho, Thien Quito-Avila, Diego Keller, Karen E. Postman, Joseph D. Martin, Robert R. Tzanetakis, Ioannis E. TI Evidence of sympatric speciation of elderberry carlaviruses SO VIRUS RESEARCH LA English DT Article DE Discovery; Evolution; Universal carlavirus detection ID SAP-TRANSMISSIBLE VIRUSES; BLUEBERRY-SCORCH; GENERATION; IDENTIFICATION; SEQUENCES; SAMBUCUS; DISEASES; DATABASE; GENOME; TREES AB Five new carlaviruses infecting elderberry were characterized and tentatively named as elderberry virus A-E (EIVA-EIVE). Their genome organization is similar to that of other carlaviruses with size ranging from 8540 to 8628 nucleotides, excluding the polyadenylated tails. EIVA, EIVB and EIVD share a common ancestor as do EIVC and EWE, indicating that speciation may be sympatric with all viruses having emerged in elderberry. Analyses of the carlavirus conserved domains indicate that the 2-oxoglutarate and Fe(II)-dependent oxygenase motifs are reliable indicators of virus phylogenetic classification with recombination playing a significant role in the evolution of the genus. A universal RT-PCR assay that detects all the elderberry carlaviruses and potentially other members of the genus has been developed. This tool can be used for research and regulatory purposes as elderberry cultivation is rapidly expanding to new areas where the viruses may be absent. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ho, Thien; Tzanetakis, Ioannis E.] Univ Arkansas Syst, Dept Plant Pathol, Div Agr, Fayetteville, AR 72701 USA. [Keller, Karen E.; Martin, Robert R.] USDA ARS, Corvallis, OR 97330 USA. [Postman, Joseph D.] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. [Quito-Avila, Diego] Escuela Super Politecn Litoral ESPOL, CIBE, EC-090150 Guayaquil, Guayas, Ecuador. RP Tzanetakis, IE (reprint author), Univ Arkansas Syst, Dept Plant Pathol, Div Agr, Fayetteville, AR 72701 USA. EM itzaneta@uark.edu RI Ho, Thien/A-7209-2012 OI Ho, Thien/0000-0003-0914-306X FU USDA NCPN [14-8130-0420-CA, 14-8130-0392-CA]; Hatch project [1002361] FX This work was supported, at least in part, by USDA NCPN grants 14-8130-0420-CA, 14-8130-0392-CA and Hatch project1002361. NR 39 TC 1 Z9 1 U1 4 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD APR 2 PY 2016 VL 215 BP 72 EP 75 DI 10.1016/j.virusres.2016.01.017 PG 4 WC Virology SC Virology GA DH3IP UT WOS:000372681000010 PM 26851177 ER PT J AU DeSmet, A Thompson, D Baranowski, T Palmeira, A Verloigne, M De Bourdeaudhuij, I AF DeSmet, Ann Thompson, Debbe Baranowski, Tom Palmeira, Antonio Verloigne, Maite De Bourdeaudhuij, Ilse TI Is Participatory Design Associated with the Effectiveness of Serious Digital Games for Healthy Lifestyle Promotion? A Meta-Analysis SO JOURNAL OF MEDICAL INTERNET RESEARCH LA English DT Article DE serious games; video games; computer games; games; health promotion; meta-analysis; review; design; community-based participatory research ID RANDOMIZED CONTROLLED-TRIAL; EDUCATIONAL COMPUTER GAMES; RECEIVING FOOD ASSISTANCE; MIDDLE SCHOOL YOUTH; PHYSICAL-ACTIVITY; FORMATIVE RESEARCH; OUTCOME EVALUATION; RISK REDUCTION; PROGRAM; INTERVENTIONS AB Background: Serious digital games can be effective at changing healthy lifestyles, but large differences in their effectiveness exist. The extent of user involvement in game design may contribute to game effectiveness by creating a better fit with user preferences. Participatory design (PD), which represents active user involvement as informant (ie, users are asked for input and feedback) or codesigner (ie, users as equal partners in the design) early on and throughout the game development, may be associated with higher game effectiveness, as opposed to no user involvement or limited user involvement. Objective: This paper reports the results of a meta-analysis examining the moderating role of PD in the effectiveness of serious digital games for healthy lifestyle promotion. Methods: Four databases were searched for peer-reviewed papers in English that were published or in press before October 2014, using a (group-) randomized controlled trial design. Effectiveness data were derived from another meta-analysis assessing the role of behavior change techniques and game features in serious game effectiveness. Results: A total of 58 games evaluated in 61 studies were included. As previously reported, serious digital games had positive effects on healthy lifestyles and their determinants. Unexpectedly, PD (g=0.075, 95% CI 0.017 to 0.133) throughout game development was related to lower game effectiveness on behavior (Q=6.74, P<.05) than when users were only involved as testers (g=0.520, 95% CI 0.150 to 0.890, P<.01). Games developed with PD (g=0.171, 95% CI 0.061 to 0.281, P<.01) were also related to lower game effectiveness on self-efficacy (Q=7.83, P<.05) than when users were not involved in game design (g=0.384, 95% CI 0.283 to 0.485, P<.001). Some differences were noted depending on age group, publication year of the study, and on the specific role in PD (ie, informant or codesigner), and depending on the game design element. Games developed with PD were more effective in changing behavioral determinants when they included users in design elements on game dynamics (beta=.215, 95% CI .075 to .356, P<.01) and, more specifically, as an informant (beta=.235, 95% CI .079 to .329, P<.01). Involving users as informants in PD to create game levels was also related to higher game effectiveness (Q=7.02, P<.01). Codesign was related to higher effectiveness when used to create the game challenge (Q=11.23, P<.01), but to lower game effectiveness when used to create characters (Q=4.36, P<.05) and the game world (Q=3.99, P<.05). Conclusions: The findings do not support higher effectiveness of games developed with PD. However, significant differences existed among PD games. More support was found for informant roles than for codesign roles. When PD was applied to game dynamics, levels, and game challenge, this was associated with higher effectiveness than when it was applied to game aesthetics. Since user involvement may have an important influence on reach, adoption, and implementation of the intervention, further research and design efforts are needed to enhance effectiveness of serious games developed with PD. C1 [DeSmet, Ann; Verloigne, Maite; De Bourdeaudhuij, Ilse] Univ Ghent, Dept Movement & Sport Sci, Watersportlaan 2, B-9000 Ghent, Belgium. [Thompson, Debbe; Baranowski, Tom] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Palmeira, Antonio] Univ Lisbon, Univ Lusofona Humanidades & Tecnol, Fac Phys Educ & Sports, Lisbon, Portugal. [Palmeira, Antonio] Fac Human Movement, Interdisciplinary Ctr Study Human Performance CIP, Lisbon, Portugal. RP De Bourdeaudhuij, I (reprint author), Univ Ghent, Dept Movement & Sport Sci, Watersportlaan 2, B-9000 Ghent, Belgium. EM ilse.debourdeaudhuij@ugent.be OI Palmeira, Antonio/0000-0001-6508-0599; Thompson, Debbe/0000-0002-5491-8816; Baranowski, Tom/0000-0002-0653-2222; De Bourdeaudhuij, Ilse/0000-0001-9969-7597 FU Flemish Agency for Innovation by Science and Technology [110051]; Research Foundation Flanders (FWO) [FWO13/PDO/191]; US Department of Agriculture; United States Department of Agriculture/Agricultural Research Service (USDA/ARS) [3092-5-001-058] FX This study received a grant (No. 110051) from the Flemish Agency for Innovation by Science and Technology. MV is supported by the Research Foundation Flanders (FWO) with a postdoctoral research fellowship (FWO13/PDO/191). DT and TB are supported by the US Department of Agriculture, funded in part with federal funds from the United States Department of Agriculture/Agricultural Research Service (USDA/ARS) under Cooperative Agreement number 3092-5-001-058. The authors wish to thank Wendy Van Lippevelde for her assistance in double coding, and all authors of the included studies for providing supplementary information when requested. NR 85 TC 1 Z9 1 U1 6 U2 6 PU JMIR PUBLICATIONS, INC PI TORONTO PA 59 WINNERS CIRCLE, TORONTO, ON M4L 3Y7, CANADA SN 1438-8871 J9 J MED INTERNET RES JI J. Med. Internet Res. PD APR PY 2016 VL 18 IS 4 AR e94 DI 10.2196/jmir.4444 PG 19 WC Health Care Sciences & Services; Medical Informatics SC Health Care Sciences & Services; Medical Informatics GA EC9VZ UT WOS:000388493700011 PM 27129447 ER PT J AU Brown, J Colson, G de La Serre, CB Magnan, N AF Brown, Joanna Colson, Gregory de La Serre, Claire B. Magnan, Nicholas TI Summer Garden Programs Improve Children's Food Knowledge and Preferences: Evidence Using Stated and Revealed Preference Measures SO HORTTECHNOLOGY LA English DT Article DE food choice experiment; healthy food choices ID PARENTAL SOCIAL SUPPORT; VEGETABLE CONSUMPTION; OBESITY PREVENTION; NUTRITION; BENEFITS; HEALTH; FRUIT; YOUTH AB One reason for excessive body weight among youth is poor eating choices. Garden-based programs have the potential to educate children about fruits and vegetables and healthy eating generally, and improve their food preferences. This study examines the impacts of two community-based summer garden programs in Atlanta, GA, on children aged 5-14 years (n = 71). These programs spanned 1 to 2 weeks and included daily gardening activities and nutritional education. The study employs a pre-and postprogram questionnaire and a food choice experiment to evaluate changes in knowledge about and preferences for healthy and unhealthy foods. Results show that the programs substantially and significantly increased knowledge about nutrients (P < 0.01), plants (P < 0.1), and healthy foods (P < 0.01). The programs also increased the number of fruits and vegetables participants tried (P < 0.1) and their propensity to make healthy choices in the food experiment (P < 0.1). Regression analysis shows program impacts on plant knowledge (P < 0.1) and fruits and vegetables tried (P < 0.1) to be lower for African American children (n = 38) and all other program impacts to be statistically homogenous. At least in the short term, garden programs such as these can alter children's preferences and decisions regarding healthy eating. More research is needed to see if these effects persist and ultimately improve health outcomes into adulthood. C1 [Brown, Joanna] Foreign Agr Serv, USDA, Room 5532,1400 Independence Ave SW, Washington, DC 20250 USA. [Colson, Gregory; Magnan, Nicholas] Univ Georgia, Dept Agr & Appl Econ, 301 Conner Hall, Athens, GA 30602 USA. [de La Serre, Claire B.] Univ Georgia, Dept Foods & Nutr, 280 Dawson Hall, Athens, GA 30602 USA. RP Magnan, N (reprint author), Univ Georgia, Dept Agr & Appl Econ, 301 Conner Hall, Athens, GA 30602 USA. EM nmagnan@uga.edu FU University of Georgia FX This research was funded by a Faculty Research Grant from the University of Georgia. NR 26 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD APR PY 2016 VL 26 IS 2 BP 133 EP 140 PG 8 WC Horticulture SC Agriculture GA DY3IC UT WOS:000384983400003 ER PT J AU Mcgee, TK Curtis, A McFarlane, BL Shindler, B Christianson, A Olsen, C McCaffrey, S AF Mcgee, Tara K. Curtis, Allan McFarlane, Bonita L. Shindler, Bruce Christianson, Amy Olsen, Christine McCaffrey, Sarah TI Facilitating knowledge transfer between researchers and wildfire practitioners about trust: An international case study SO FORESTRY CHRONICLE LA English DT Article DE knowledge transfer; wildfire; trust; wildfire management AB The importance of knowledge transfer between researchers, policy makers and practitioners is widely recognized. However, barriers to knowledge transfer can make it difficult for practitioners to apply the results of scientific research. This paper describes a project that addressed barriers to knowledge transfer by involving wildfire management practitioners from three countries in developing a trust planning guide. The guide provides information about trust, factors that influence trust and actions that can be taken to build trust in the context of wildfire management. The researchers synthesized academic research into a draft trust planning guide. Wildfire management practitioners and stakeholders provided feedback about the guide and discussed their own experiences in building trust in a workshop setting. The researchers incorporated valuable feedback from the workshops into the final trust planning guide. Benefits and challenges of this process are discussed, and the authors provide recommendations for researchers and funding agencies to facilitate the uptake of research by end-users. C1 [Mcgee, Tara K.] Univ Alberta, Dept Earth & Atmospher Sci, Edmonton, AB, Canada. [Curtis, Allan] Charles Sturt Univ, Inst Land Water & Soc, Bathurst, NSW 2795, Australia. [McFarlane, Bonita L.; Christianson, Amy] Canadian Forest Serv, Nat Resources Canada, Edmonton, AB, Canada. [Shindler, Bruce; Olsen, Christine] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [McCaffrey, Sarah] US Forest Serv, USDA, Northern Res Stn, Washington, DC USA. RP Mcgee, TK (reprint author), Univ Alberta, Dept Earth & Atmospher Sci, Edmonton, AB, Canada. EM tmcgee@ualberta.ca FU Joint Fire Science Program [10-3-01-25] FX The authors would like to thank the workshop participants for providing us with extremely valuable feedback on the draft planning guide and insights into how they build trust on the ground. Special thanks to the agency representatives in each country that helped to recruit workshop participants and organized the field visits for the research team. Thank you to the Joint Fire Science Program for funding this project (ID# 10-3-01-25) and helping to disseminate the trust guide to fire practitioners. NR 13 TC 0 Z9 0 U1 3 U2 3 PU CANADIAN INST FORESTRY PI MATTAWA PA C/O CANADIAN ECOLOGY CENTRE, PO BOX 430, 6905 HWY 17 W, MATTAWA, ONTARIO P0H 1V0, CANADA SN 0015-7546 EI 1499-9315 J9 FOREST CHRON JI For. Chron. PD APR PY 2016 VL 92 IS 2 BP 167 EP 171 DI 10.5558/tfc2016-035 PG 5 WC Forestry SC Forestry GA DW9MC UT WOS:000383983100014 ER PT J AU Taskin, H Dogan, HH Buyukalaca, S Clowez, P Moreau, PA O'Donnell, K AF Taskin, Hatira Dogan, Hasan Huseyin Buyukalaca, Saadet Clowez, Philippe Moreau, Pierre-Arthur O'Donnell, Kerry TI Four new morel (Morchella) species in the elata subclade (M. sect. Distantes) from Turkey SO MYCOTAXON LA English DT Article DE Ascomycota; conservation; edible fungi; Morchellaceae; systematics; taxonomy ID TRUE; ASCOMYCOTA; PEZIZALES; REVEALS; EUROPE; NOV AB Four Turkish Morchella species identified in published multilocus molecular phylogenetic analyses are described here as new, using detailed macro-and microscopic data: M. mediterraneensis (Mel-27), M. fekeensis (Mel-28), M. magnispora (Mel-29), and M. conifericola (Mel-32). A distribution map of morels identified to date in Turkey is also provided. C1 [Taskin, Hatira; Buyukalaca, Saadet] Univ Cukurova, Fac Agr, Dept Hort, TR-01330 Adana, Turkey. [Dogan, Hasan Huseyin] Selcuk Univ, Fac Sci, Dept Biol, TR-42079 Konya, Turkey. [Clowez, Philippe] 56 Pl Tilleuls, F-60400 Pont Leveque, France. [Moreau, Pierre-Arthur] Univ Lille, UFR Pharm, EA 4483, F-59000 Lille, France. [O'Donnell, Kerry] ARS, USDA, Natl Ctr Agr Utilizat Res, Mycotoxin Prevent & Appl Mycol Res Unit, 1815 North Univ St, Peoria, IL 61604 USA. RP Taskin, H (reprint author), Univ Cukurova, Fac Agr, Dept Hort, TR-01330 Adana, Turkey. EM hatirataskin1@gmail.com FU Scientific and Technological Research Council of Turkey (TUBITAK); Cukurova University, Scientific Research Projects Coordinating Office [CU-BAP-ZF2009D41] FX HT and SB would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) and Cukurova University, Scientific Research Projects Coordinating Office (CU-BAP-ZF2009D41) for supporting this research. Thanks are also due to Donald Pfister, Stephen Rehner, and Andrus Voitk for reviewing this submission and Manuel Becerra Parra for his Spanish collection of Morchella mediterraneensis. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. NR 17 TC 0 Z9 0 U1 2 U2 2 PU MYCOTAXON LTD PI ITHACA PA PO BOX 264, ITHACA, NY 14851-0264 USA SN 0093-4666 J9 MYCOTAXON JI Mycotaxon PD APR-JUN PY 2016 VL 131 IS 2 BP 467 EP 482 DI 10.5248/131.467 PG 16 WC Mycology SC Mycology GA DW7RG UT WOS:000383848200029 ER PT J AU Culley, TM Huebner, CD Novy, A AF Culley, Theresa M. Huebner, Cynthia D. Novy, Ari TI Regional and Local Genetic Variation in Japanese Stiltgrass (Microstegium vimineum) SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Genetic structure; invasive; local dispersal; microsatellites ID CANOPY DECIDUOUS FORESTS; MULTILOCUS GENOTYPE DATA; CENTRAL HARDWOOD FOREST; INVASIVE GRASS; C-4 GRASS; ENVIRONMENTAL GRADIENTS; POPULATION-STRUCTURE; PLANT-COMMUNITIES; SHADE-TOLERANT; VARIABLE LIGHT AB Nonnative M. vimineum has been expanding rapidly in the eastern United States, where it can negatively affect plant communities. Locally, the species is assumed to spread from roadsides into nearby forests, where it can form dense populations after disturbances, especially in light gaps. Using microsatellite markers, we quantified patterns of genetic variation and structure among populations at nine sites in West Virginia. We then examined patterns of local dispersal within each population, focusing on subpopulations along the roadside, those coalescing nearby along the forest edge, and subpopulations in the interior forest. We found that levels of genetic variation of M. vimineum were relatively low overall across populations but with genetic structure present among populations (F-st = 0.60). Within populations, subpopulations along the roadside were genetically variable, containing 4 to 22 unique, multilocus genotypes. Many of these genotypes were also identified in the adjacent forest, consistent with local, diffusive spread from the roadway. However, several genotypes in the interior forest were unique to the population, indicating that dispersal from other sites may also occur. Overall, it appears that genetic diversity and structure in M. vimineum reflects a variety of processes, including localized dispersal and long-distance migration. Nomenclature: Japanese stiltgrass, Microstegium vimineum (Trin.) A. Camus. C1 [Culley, Theresa M.] Univ Cincinnati, Dept Biol Sci, 614 Rieveschl Hall, Cincinnati, OH 45221 USA. US Forest Serv, USDA, Northern Res Stn, 180 Canfield St, Morgantown, WV 26505 USA. US Bot Garden, 245 First St SW, Washington, DC 20024 USA. Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, Washington, DC 20560 USA. RP Culley, TM (reprint author), Univ Cincinnati, Dept Biol Sci, 614 Rieveschl Hall, Cincinnati, OH 45221 USA. EM theresa.culley@uc.edu FU Northern Research Station, U.S. Department of Agriculture, Forest Service, Morgantown, WV [10-JV11242303-047] FX The authors thank L. Chaney for field collection of samples, as well as S. Stancheva, S. Yadav, and S. Albers for assistance with the genetic analysis of the PRD and WVU populations. We thank several anonymous reviewers for their valuable comments. This research was funded by the Northern Research Station, U.S. Department of Agriculture, Forest Service, Morgantown, WV, with Joint Venture Agreement 10-JV11242303-047. NR 64 TC 0 Z9 0 U1 7 U2 7 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2016 VL 9 IS 2 BP 96 EP 111 DI 10.1614/IPSM-D-15-00055.1 PG 16 WC Plant Sciences SC Plant Sciences GA DS2EM UT WOS:000380561300002 ER PT J AU Boswell, A Sing, SE Ward, SM AF Boswell, Andrew Sing, Sharlene E. Ward, Sarah M. TI Plastid DNA Analysis Reveals Cryptic Hybridization in Invasive Dalmatian Toadflax (Linaria dalmatica) Populations SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE cpDNA; DNA barcoding; cryptic hybridization; gene flow; haplotype ID CHLOROPLAST DNA; VULGARIS SCROPHULARIACEAE; UNITED-STATES; DISCRIMINATION; INHERITANCE; POLLINATION; PHYLOGENY; SEQUENCES; L. AB Gene flow between Dalmatian toadflax (DT) and yellow toadflax (YT), both aggressive invaders throughout the Intermountain West, is creating hybrid populations potentially more invasive than either parent species. To determine the direction of gene flow in these hybrid populations, species-diagnostic cytoplasmic markers were developed. Markers were based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) polymorphisms in the trnT-D chloroplast DNA (cpDNA) region digested with Alu1, and single-nucleotide polymorphisms (SNPs) in the matK and trnL-F chloroplast-barcoding regions. Four hybrid toadflax populations sampled from Colorado, Montana, and Washington contained both DT and YT cytoplasm, with YT predominating; 25 individuals from a fifth hybrid population from Idaho all had identical YT cpDNA haplotypes. Thirteen plants from two Colorado populations, assumed to be DT based on morphology and geographic isolation from any known YT population, were found to have YT cpDNA haplotypes. These results indicate that gene flow between invasive YT and DT populations is more widespread that previously realized and confirms that cryptic introgression of YT alleles has occurred in multiple western U.S. DT populations. The presence of YT genetic material in presumed DT populations may negatively affect host recognition and establishment by biocontrol agents used for toadflax management. Nomenclature: Dalmatian toadflax, Linaria dalmatica (L.) P. Mill.; yellow toadflax, Linaria vulgaris P. Mill. C1 [Boswell, Andrew; Ward, Sarah M.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Sing, Sharlene E.] US Forest Serv, USDA, Rocky Mt Res Stn, Bozeman, MT 59717 USA. RP Ward, SM (reprint author), Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. EM sarah.ward@colostate.edu FU U.S. Department of Agriculture (USDA)-Forest Service, Rocky Mountain Research Station [JVA 10-JV-11221632-225]; USDA-National Institute of Food and Agriculture, Agricultural Experiment Station project [COL00625]; Colorado State University Program in Molecular Plant Biology FX This research was supported by funds provided by the U.S. Department of Agriculture (USDA)-Forest Service, Rocky Mountain Research Station, under JVA 10-JV-11221632-225, and by USDA-National Institute of Food and Agriculture, Agricultural Experiment Station project COL00625. A.B. received financial support from the Colorado State University Program in Molecular Plant Biology. NR 41 TC 0 Z9 0 U1 6 U2 6 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2016 VL 9 IS 2 BP 112 EP 120 DI 10.1614/IPSM-D-16-00003.1 PG 9 WC Plant Sciences SC Plant Sciences GA DS2EM UT WOS:000380561300003 ER PT J AU Shi, XY Lin, H AF Shi, Xiangyang Lin, Hong TI Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Immunology; Issue 110; Xylella fastidiosa; twitch motility; type IV pilus; pilG; microfluidic; flow chamber ID IV PILI; PSEUDOMONAS-AERUGINOSA; BIOFILM FORMATION; ADHESION; EXTENSION; PATHOGEN; FORCES AB Xylella fastidiosa is a Gram-negative non-flagellated bacterium that causes a number of economically important diseases of plants. The twitching motility provides X. fastidiosa a means for long-distance intra-plant movement and colonization, contributing toward pathogenicity in X. fastidiosa. The twitching motility of X. fastidiosa is operated by type IV pili. Type IV pili of Xylella fastidiosa are regulated by pilG, a chemotaxis regulator in Pil-Chp operon encoding proteins that are involved with signal transduction pathways. To elucidate the roles of pilG in the twitching motility of X. fastidiosa, a pilG-deficient mutant Xf Delta pilG and its complementary strain Xf Delta pilG-C containing native pilG were developed. A microfluidic chambers integrated with a time-lapse image recording system was used to observe twitching motility in Xf Delta pilG, Xf Delta pilG-C and its wild type strain. Using this recording system, it permits long-term spatial and temporal observations of aggregation, migration of individual cells and populations of bacteria via twitching motility. X. fastidiosa wild type and complementary Xf Delta pilG-C strain showed typical twitching motility characteristics directly observed in the microfluidic flow chambers, whereas mutant Xf Delta pliG exhibited the twitching deficient phenotype. This study demonstrates that pilG contributes to the twitching motility of X. fastidiosa. The microfluidic flow chamber is used as a means for observing twitching motility. C1 [Shi, Xiangyang] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Lin, Hong] ARS, San Joaquin Valley Agr Sci Ctr, USDA, Washington, DC USA. RP Lin, H (reprint author), ARS, San Joaquin Valley Agr Sci Ctr, USDA, Washington, DC USA. EM Hong.Lin@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This study was supported by United States Department of Agriculture, Agricultural Research Service. Trade names or commercial products in this publication are mentioned solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 0 Z9 0 U1 3 U2 3 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD APR PY 2016 IS 110 AR e53816 DI 10.3791/53816 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR9ZX UT WOS:000380256000046 ER PT J AU Dowell, RV Jang, EB AF Dowell, Robert V. Jang, Eric B. TI Attraction of nontarget insects to a monofluoro analog of methyl eugenol in California SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Regulatory trapping; eradication treatments ID ORIENTAL FRUIT-FLY; BACTROCERA-DORSALIS AB This study evaluated the attraction of insects previously shown to be attracted to methyl eugenol to a monofluorinated analog of methyl eugenol. Orchesia Latreille, 1807 (Coleoptera: Melandryidae), an Empis Linneaus, 1758 (Diptera: Empididae) and six Syrphidae (Diptera) (Allograpta obliqua (Say, 1823), Blera scitula Williston, 1881, Hadromyia pulchra Williston, 1882, Ferdinandea Rondani, 1884, Melanosoma mellinum (Linnaeus, 1758) and Platycheirus LePeleteier & Serville, 1828) previously shown to be attracted to methyl eugenol were found to be attracted to the monofluorinated methyl eugenol analog. Three additional Diptera: Syrphidae, Dasysyrphus Enderlien,1938, Pseudoscaeva diversifasciatus Knab, 1914 and Blera humeralis Williston, 1882) were deemed to be attracted to the monofluorinated methyl eugenol. A number of insects attracted to methyl eugenol were not attracted to monofluorinated methyl eugenol including Diptera in Lauxaniidae, Scatopsidae, Mycetophilidae, and Drosphilidae, Lepidoptera in Noctuidae, Tortricidae and Pyralidae, a Hemiptera in Miridae, and Coleoptera in Chrysomelidae, Cantharidae, Cerambycidae, Elataeridae and Mordellidae. C1 [Dowell, Robert V.] Calif Dept Food & Agr, Sacramento, CA 95814 USA. [Jang, Eric B.] USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Dowell, RV (reprint author), Calif Dept Food & Agr, Sacramento, CA 95814 USA. EM Bdowell396@gmail.com FU Farm Bill [13-8130-0132] FX This work was partially supported by Farm Bill contract number 13-8130-0132. Kevin Hoffman, Jason Leathers, Grant McQuate and Roger Vargas reviewed and improved earlier drafts of this manuscript. This paper reports the results of research only. Mention of a proprietary product does not constitute endorsement or recommendation by the USDA or CDFA. The USDA is an equal opportunity provider and employer. NR 14 TC 0 Z9 0 U1 1 U2 1 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2016 VL 92 IS 2 BP 79 EP 85 PG 7 WC Entomology SC Entomology GA DS1OK UT WOS:000380365600002 ER PT J AU Frey, SJK Hadley, AS Johnson, SL Schulze, M Jones, JA Betts, MG AF Frey, Sarah J. K. Hadley, Adam S. Johnson, Sherri L. Schulze, Mark Jones, Julia A. Betts, Matthew G. TI Spatial models reveal the microclimatic buffering capacity of old-growth forests SO SCIENCE ADVANCES LA English DT Article ID RESOLUTION GLOBAL-MAPS; CLIMATE-CHANGE; PACIFIC-NORTHWEST; TEMPERATE FOREST; COVER CHANGE; HABITAT; EDGE; CONSERVATION; TOPOGRAPHY; ECOSYSTEMS AB Climate change is predicted to cause widespread declines in biodiversity, but these predictions are derived from coarse-resolution climate models applied at global scales. Such models lack the capacity to incorporate microclimate variability, which is critical to biodiversity microrefugia. In forested montane regions, microclimate is thought to be influenced by combined effects of elevation, microtopography, and vegetation, but their relative effects at fine spatial scales are poorly known. We used boosted regression trees to model the spatial distribution of fine-scale, under-canopy air temperatures in mountainous terrain. Spatial models predicted observed independent test data well (r = 0.87). As expected, elevation strongly predicted temperatures, but vegetation and microtopography also exerted critical effects. Old-growth vegetation characteristics, measured using LiDAR (light detection and ranging), appeared to have an insulating effect; maximum spring monthly temperatures decreased by 2.5 degrees C across the observed gradient in old-growth structure. These cooling effects across a gradient in forest structure are of similar magnitude to 50-year forecasts of the Intergovernmental Panel on Climate Change and therefore have the potential to mitigate climate warming at local scales. Management strategies to conserve old-growth characteristics and to curb current rates of primary forest loss could maintain microrefugia, enhancing biodiversity persistence in mountainous systems under climate warming. C1 [Frey, Sarah J. K.; Hadley, Adam S.; Schulze, Mark; Betts, Matthew G.] Oregon State Univ, Dept Forest Ecosyst & Soc, Forest Biodivers Res Network, Corvallis, OR 97331 USA. [Johnson, Sherri L.] US Forest Serv, Pacific Northwest Res Stn, Corvallis, OR 97331 USA. [Jones, Julia A.] Oregon State Univ, CEOAS, Geog, Corvallis, OR 97331 USA. RP Frey, SJK; Betts, MG (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Forest Biodivers Res Network, Corvallis, OR 97331 USA. EM sarah.frey@oregonstate.edu; matt.betts@oregonstate.edu NR 65 TC 5 Z9 5 U1 9 U2 15 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 2375-2548 J9 SCI ADV JI Sci. Adv. PD APR PY 2016 VL 2 IS 4 AR e1501392 DI 10.1126/sciadv.1501392 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR7IB UT WOS:000380072100019 PM 27152339 ER PT J AU Washburn, BE Elbin, SB Davis, C AF Washburn, Brian E. Elbin, Susan B. Davis, Christina TI Historical and Current Population Trends of Herring Gulls (Larus argentatus) and Great Black-backed Gulls (Larus marinus) in the New York Bight, USA SO WATERBIRDS LA English DT Article DE Great Black-backed Gull; Herring Gull; Larus argentatus; Larus marinus; nesting colonies; New York Bight ID JAMAICA-BAY; COLONIAL WATERBIRDS; BREEDING SUCCESS; LAUGHING-GULLS; URBAN ESTUARY; NEW-JERSEY; CITY; MANAGEMENT; AERIAL; ABUNDANCE AB During the 20th century, gull populations in North America experienced considerable changes in abundance and geographic ranges. The objective of this study was to describe population trends of Herring Gulls (Larus argentatus) and Great Black-backed Gulls (L. marinus) in the New York Bight, USA, over a 40-year period (1974-2013). A variety of data sources using different survey methods provided estimates of the number of breeding pairs for both species. In the Long Island portion of the New York Bight, overall Herring and Great Black-backed gull nesting populations appear to have fluctuated considerably in size during this time period, and the largest numbers of breeding individuals of these two species occurred in the 1980s. In coastal New Jersey, the Herring Gull nesting population has remained relatively constant, whereas the Great Black-backed Gull nesting population has increased. Individual nesting colonies are dynamic and can vary in size considerably during even short time periods. Several factors, including sea-level changes and the availability of anthropogenic food sources (i.e., at landfills and fisheries by-catch), likely have strongly influenced individual colonies and the overall Herring and Great Black-backed gull breeding populations in the New York Bight. C1 [Washburn, Brian E.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. [Elbin, Susan B.] New York City Audubon, 71 West 23rd St, New York, NY 10010 USA. [Davis, Christina] New Jersey Div Wildlife, 2201 Cty Route 631, Woodbine, NJ 08270 USA. RP Washburn, BE (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov FU U.S. Department of Agriculture; New York City Audubon; New Jersey Division of Wildlife; National Park Service FX We thank the U.S. Department of Agriculture, New York City Audubon, New Jersey Division of Wildlife, and National Park Service for their financial and logistical support. The New York State Department of Environmental Conservation, especially F. Hamilton and M. Gibbons, and the New Jersey Department of Environmental Protection were very supportive in providing data and assistance. We are indebted to the numerous volunteers, wildlife biologists and technicians, and others who assisted both New York and New Jersey with census efforts and conservation efforts for decades. T. DeVault, J. Wash-burn, and two anonymous reviewers provided helpful comments that improved the manuscript. NR 59 TC 1 Z9 1 U1 3 U2 3 PU WATERBIRD SOC PI WASHINGTON PA NATL MUSEUM NATURAL HISTORY SMITHSONIAN INST, WASHINGTON, DC 20560 USA SN 1524-4695 EI 1938-5390 J9 WATERBIRDS JI Waterbirds PD APR PY 2016 VL 39 SI 1 BP 74 EP 86 PG 13 WC Ornithology SC Zoology GA DR4PF UT WOS:000379882900009 ER PT J AU Flores, R Owens, RA Taylor, J AF Flores, Ricardo Owens, Robert A. Taylor, John TI Pathogenesis by subviral agents: viroids and hepatitis delta virus SO CURRENT OPINION IN VIROLOGY LA English DT Article ID VIRULENCE MODULATING REGION; INFECTED TOMATO PLANTS; RNA SILENCING PATHWAYS; B-VIRUS; IN-VIVO; NUCLEOTIDE-SEQUENCE; HOST INTERACTIONS; INTERFERING RNAS; NONCODING RNA; CIRCULAR RNAS AB The viroids of plants are the simplest known infectious genetic elements. They have RNA genomes of up to 400 nucleotides in length and no protein encoding capacity. Hepatitis delta virus (HDV), an infectious agent found only in humans co-infected with hepatitis B virus (HBV), is just slightly more complex, with an RNA genome of about 1700 nucleotides, and the ability to express just one small protein. Viroid and HDV RNAs share several features that include circular structure, compact folding, and replication via a rolling-circle mechanism. Both agents were detected because of their obvious pathogenic effects. Their simplicity demands a greater need than conventional RNA or DNA viruses to redirect host components for facilitating their infectious cycle, a need that directly and indirectly incites pathogenic effects. The mechanisms by which these pathogenic effects are produced are the topic of this review. In this context, RNA silencing mediates certain aspects of viroid pathogenesis. C1 [Flores, Ricardo] Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, E-46022 Valencia, Spain. [Owens, Robert A.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Taylor, John] Fox Chase Canc Ctr, Philadelphia, PA 19111 USA. RP Flores, R (reprint author), Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, E-46022 Valencia, Spain.; Owens, RA (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA.; Taylor, J (reprint author), Fox Chase Canc Ctr, Philadelphia, PA 19111 USA. EM rflores@ibmcp.upv.es; owensj301@hotmail.com; John.Taylor@fccc.edu RI Flores, Ricardo/K-2788-2014 OI Flores, Ricardo/0000-0002-3033-5077 FU Spanish Ministerio de Economia y Competitividad [BFU2014-56812-P] FX We apologize to colleagues whose work could not be cited for space limits. Work in RF laboratory has been supported by the Spanish Ministerio de Economia y Competitividad (grant BFU2014-56812-P). NR 104 TC 3 Z9 3 U1 8 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1879-6257 J9 CURR OPIN VIROL JI Curr. Opin. Virol. PD APR PY 2016 VL 17 BP 87 EP 94 DI 10.1016/j.coviro.2016.01.022 PG 8 WC Virology SC Virology GA DQ1LU UT WOS:000378963300015 PM 26897654 ER PT J AU Shin, SB Zheng, P Fazio, G Mazzola, M Main, D Zhu, YM AF Shin, Sungbong Zheng, Ping Fazio, Gennaro Mazzola, Mark Main, Dorrie Zhu, Yanmin TI Transcriptome changes specifically associated with apple (Malus domestica) root defense response during Pythium ultimum infection SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Root defense response; Transcriptomic change; Soilborne necrotrophic pathogens; Secondary metabolism; Hormone regulation ID PATHOGENESIS-RELATED PROTEINS; PLANT IMMUNITY; REPLANT DISEASE; RNA-SEQ; NECROTROPHIC PATHOGENS; PRATYLENCHUS-PENETRANS; SECONDARY METABOLITES; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; ORCHARD SOILS AB RNA-seq technology was applied to identify the transcriptomic changes associated with apple root defense response to Pythium ultimum infection. Genes encoding homolog proteins with functions of pathogen detection such as chitin elicitor receptor kinase (CERK) and wall-associated receptor kinase (WAK) were among the differentially expressed apple genes. The biosynthesis and signaling of several plant hormones including ethylene, jasmonate and cytokinin were specifically induced in response to P. ultimum inoculation. Genes encoding enzymes of secondary metabolisms, cell wall fortification and pathogenesis related (PR) protein, laccase, mandelonitrile lyase and cyanogenic beta-glucosidase were consistently up-regulated in the later stages of infection. Published by Elsevier Ltd. C1 [Shin, Sungbong; Mazzola, Mark; Zhu, Yanmin] USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. [Fazio, Gennaro] USDA ARS, Plant Genet Resources Unit, Geneva, NY 14456 USA. [Zheng, Ping; Main, Dorrie] Washington State Univ, Dept Hort, Pullman, WA 98664 USA. RP Zhu, YM (reprint author), USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM yanmin.zhu@ars.usda.gov FU USDA from Washington Tree Fruit Research Commission FX We thank Edward Valdez and Steve Reymond for their excellent technical assistance. The authors also thank Melody Saltzgiver, Connor Foli for their proof reading the article. This work was supported by USDA base fund and the research fund from Washington Tree Fruit Research Commission. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. NR 76 TC 1 Z9 1 U1 10 U2 12 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2016 VL 94 BP 16 EP 26 DI 10.1016/j.pmpp.2016.03.003 PG 11 WC Plant Sciences SC Plant Sciences GA DQ4FD UT WOS:000379158400004 ER PT J AU Rashid, A AF Rashid, Abdur TI Defense responses of plant cell wall non-catalytic proteins against pathogens SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Plant cell wall; Non-enzymatic proteins; Pathogen; Biological elicitation; Wounding; Defense ID HYDROXYPROLINE-RICH GLYCOPROTEIN; OXIDATIVE CROSS-LINKING; ARABIDOPSIS EXTENSIN GENE; ARABINOGALACTAN-PROTEINS; PROLINE-RICH; DISEASE RESISTANCE; FUNGAL ELICITOR; BRASSICA-NAPUS; MESSENGER-RNA; NICOTIANA-SYLVESTRIS AB Plant cell wall (CW) associated non-enzymatic proteins (CWPs) are composed mainly of glycoproteins containing a polypeptide backbone attached with carbohydrate side chains. These CWPs typically include hydroxyproline-rich glycoproteins (HRGPs), proline-rich proteins (PRPs), glycine-rich proteins (GRPs), and arabinogalactan proteins (AGPs). The CWPs have been implicated in plant defense against pathogens. The defense responses appear to be accomplished by several mechanisms. The major mechanisms include: (i) CW hardening through insolubilization and oxidative cross-linking of extensins and PRPs mediated via H2O2 and peroxidases, (ii) secretion and agglutination of AGPs at the sites of pathogenic infection, (iii) degradation of the genetic materials of the pathogens by binding of GRPs to the RNA of the pathogens, and (iv) activation of the pathogenesis-related (PR) gene expression using AGPs as a soluble molecular signal. This review summarizes the aforementioned defense responses, provides an update on classification, and explores future research opportunities of CWPs. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Rashid, Abdur] USDA ARS, Natl Small Grains & Potato Germplasm Res, Aberdeen, ID 83210 USA. RP Rashid, A (reprint author), Univ Alberta, Dept Biol Sci, Edmonton, AB TG6 2E9, Canada. EM abdur.rashid@ars.usda.gov NR 130 TC 0 Z9 0 U1 1 U2 3 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2016 VL 94 BP 38 EP 46 DI 10.1016/j.pmpp.2016.03.009 PG 9 WC Plant Sciences SC Plant Sciences GA DQ4FD UT WOS:000379158400006 ER PT J AU Mellon, JE Mattison, CP Grimm, CC AF Mellon, J. E. Mattison, C. P. Grimm, C. C. TI Identification of hydrolytic activities expressed by Aspergillus flavus grown on cotton carpel tissue SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Aspergillus flavus; Xylanolytic hydrolases; Pectolytic hydrolases; Virulence factors; Carpel tissue ID GENES; POLYGALACTURONASE; PATHOGENESIS; HYDROLASES; PROTEINS; NIDULANS; ENZYMES; PH AB To identify proteins important for invasion into host plant tissue, Aspergillus flavus was cultured on medium containing cotton carpel tissue as the sole carbon source. We identified several hydrolases suggesting they are important as A. flavus virulence factors for plant colonization. Specifically, Aspergillus flavus AF13 secreted at least two endoxylanase activities and a pectolytic activity when grown on the cotton carpel tissue medium. A concentrated sample derived from the A. flavus growth medium (6-day) was subjected to gel filtration chromatography on a BioGel P-30 column. A major endoxylanase activity was separated from the other fungal-secreted proteins. Additional fungal secreted proteins were partially resolved by gel filtration chromatography on a BioGel P-60 column. Multiple proteins with molecular weights in the 20 to 70 kD range were present in the harvested fungal growth medium. Analysis of these fungal-secreted proteins by liquid chromatography/tandem mass spectrometry identified both endo-and exo-glucanase proteins, a-L-arabinofuranosidase, glucoamylase, a-amylase A, pectate lyase A, xylanase F1, acetylxylan esterase, glutaminase A, as well as conserved hypothetical proteins of unknown function. These proteins likely assist A. flavus in the maceration of plant cell walls, allowing for pathogenic entry and accession of host nutrient resources. Pectolytic and xylanolytic hydrolases, as well as glucanases, appear to be important A. flavus virulence factors. Published by Elsevier Ltd. C1 [Mellon, J. E.; Mattison, C. P.; Grimm, C. C.] ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Mellon, JE (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Jay.Mellon@ars.usda.gov NR 27 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2016 VL 94 BP 67 EP 74 DI 10.1016/j.pmpp.2016.04.004 PG 8 WC Plant Sciences SC Plant Sciences GA DQ4FD UT WOS:000379158400010 ER PT J AU Webb, KM Freeman, C Broeckling, CD AF Webb, Kimberly M. Freeman, Claire Broeckling, Corey D. TI Metabolome profiling to understand the defense response of sugar beet (Beta vulgaris) to Rhizoctonia solani AG 2-2 IIIB SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Sugarbeet; Rhizoctonia crown and root rot; Host resistance; Fungal-host interactions; Plant metabolomics ID ROOT-ROT RESISTANCE; YELLOW-VEIN-VIRUS; LEAF-SPOT; PHYTOALEXINS; ANNOTATION; GENES AB Rhizoctonia crown and root rot (RCRR), caused by Rhizoctonia solani Kuhn AG 2-2 IIIB, is an important disease of sugar beet. While RCRR can be managed by agronomic practices, plant resistance remains the primary method for control. However, the molecular processes that mediate resistance to R. solani are largely unknown. The metabolic changes that occurred during susceptible and resistant R. solani interactions were compared and characterized using nontargeted metabolomic profiling. Metabolites from infected and healthy, root and leaf tissue, were taken at 0 and 7 dai and detected using reversed-phase UHPLC-MS and GC-MS. There was a clear distinction in the metabolome between tissue type and genotype, and in response to R. solani. 143 compounds were annotated and several metabolites associated with plant defense to fungi were identified in both germplasm. Published by Elsevier Ltd. C1 [Webb, Kimberly M.; Freeman, Claire] USDA ARS, Soil Management & Sugar Beet Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. [Broeckling, Corey D.] Colorado State Univ, Prote & Metabol Facil, C130 Microbiol,2021 Campus Delivery, Ft Collins, CO 80523 USA. RP Webb, KM (reprint author), USDA ARS, Soil Management & Sugar Beet Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM Kimberly.webb@ars.usda.gov FU Sugar Beet Research and Education Board of Minnesota; Beet Sugar Development Foundation; Sugar Beet Research and Education Board of North Dakota FX The authors would like to thank Amy Hill and Mia Hanson for planting and performing all inoculation treatments and processing leaf and root samples. This work was supported by the Sugar Beet Research and Education Board of Minnesota and North Dakota and the Beet Sugar Development Foundation. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 51 TC 1 Z9 1 U1 9 U2 13 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2016 VL 94 BP 108 EP 117 DI 10.1016/j.pmpp.2016.05.008 PG 10 WC Plant Sciences SC Plant Sciences GA DQ4FD UT WOS:000379158400015 ER PT J AU Borges, LMF Li, AY Olafson, PU Renthal, R Bauchan, GR Lohmeyer, KH de Leon, AAP AF Ferreira Borges, Ligia Miranda Li, Andrew Yongsheng Olafson, Pia Untalan Renthal, Robert Bauchan, Gary Roy Lohmeyer, Kimberly Hutchison Perez de Leon, Adalberto Angel TI Neuronal projections from the Haller's organ and palp sensilla to the synganglion of Amblyomma americanum SO REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA LA English DT Article DE Chemical sensilla; neuronal projection; synganglion; Amblyomma americanum ID VARIEGATUM FABRICIUS IXODIDAE; CENTRAL-NERVOUS-SYSTEM; STIMULATING OLFACTORY RECEPTORS; ANTENNAL LOBE; RHIPICEPHALUS-SANGUINEUS; ACARI IXODIDAE; 3-DIMENSIONAL RECONSTRUCTION; DROSOPHILA-MELANOGASTER; ANOPHELES-GAMBIAE; MALARIA MOSQUITO AB The present study was conducted to elucidate the neuronal pathways between peripheral olfactory and taste sensilla and the synganglion in an Ixodidae tick species. The tarsus of the front legs (olfactory nerves) and the fourth palpal segment (gustatory nerves) of unfed Amblyomma americanum males and females were excised. A neuronal tracer, dextran tetramethylrhodamine, was used for filling of the sensory neurons. The synganglion preparations were examined using a confocal microscope. Neuronal arborizations from the Haller's organ were confined to the olfactory lobes and the first pedal ganglion. The estimated number of olfactory glomeruli ranged from 16 to 22 per olfactory lobe in the females. The number of glomeruli was not counted in males because they were densely packed. Sensory neurons associated with sensilla at the distal end of the palpal organ projected into the palpal ganglion in the synganglion through the palpal nerve. Gustatory sensory neurons associated with palpal sensilla projected into a commissure with several bulges, which are confined in the palpal ganglion. The findings of distinct projection patterns of sensory neurons associated with the Haller's organ and palpal organ in the lone star tick from this study advanced our knowledge on mechanisms of sensory information processing in ticks. C1 [Ferreira Borges, Ligia Miranda] Univ Fed Goias, Dept Microbiol Imunol Parasitol & Patol, Inst Patol Trop & Saude Publ, Goiania, Go, Brazil. [Li, Andrew Yongsheng] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD USA. [Olafson, Pia Untalan; Lohmeyer, Kimberly Hutchison; Perez de Leon, Adalberto Angel] ARS, Knipling Bushland US Livestock Insects Res Lab, USDA, Vet Pest Genom Ctr, Kerrville, TX USA. [Renthal, Robert] Univ Texas San Antonio, Dept Biol, San Antonio, TX USA. [Bauchan, Gary Roy] ARS, Electron & Confocal Microscopy Unit, USDA, Beltsville, MD USA. RP Borges, LMF (reprint author), Univ Fed Goias, Dept Microbiol Imunol Parasitol & Patol, Inst Patol Trop & Saude Publ, Setor Univ, Rua 235 S-N, BR-74605050 Goiania, Go, Brazil. EM ligia@ufg.br FU Science without Borders Program - CNPq [200459/2014-7]; USDA-ARS appropriated project [3094-32000-039-00]; National Institute on Minority Health and Health Disparities [G12MD007591] FX We thank Dr. Erika Machtinger (USDA, ARS, IIBBL) for review of the manuscript, and Jason Tidwell (USDA, ARS, KBUSLIRL) for technical assistance with tick dissections. This research was supported by a Post-Doctorate Scholarship from Science without Borders Program - CNPq (200459/2014-7), with funds from USDA-ARS appropriated project 3094-32000-039-00, and by a grant to the University of Texas at San Antonio from the National Institute on Minority Health and Health Disparities (G12MD007591). NR 51 TC 1 Z9 1 U1 1 U2 1 PU BRAZILIAN COLL VETERINARY PARASITOLOGY PI SAO PAULO PA C/O ASSISTANT EDITOR AV PROF LINEU PRESTES, 1374, CIDADE UNIVERSITARIA, CEP 05508-900, SAO PAULO, 00000, BRAZIL SN 1984-2961 J9 REV BRAS PARASITOL V JI Rev. Bras. Parasitol. Vet. PD APR-JUN PY 2016 VL 25 IS 2 BP 217 EP 224 DI 10.1590/S1984-29612016039 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DP8MN UT WOS:000378752400012 PM 27334823 ER PT J AU Kantola, T Lyytikainen-Saarenmaa, P Coulson, RN Holopainen, M Tchakerian, MD Streett, DA AF Kantola, Tuula Lyytikainen-Saarenmaa, Paivi Coulson, Robert N. Holopainen, Markus Tchakerian, Maria D. Streett, Douglas A. TI Development of monitoring methods for Hemlock Woolly Adelgid induced tree mortality within a Southern Appalachian landscape with inhibited access SO IFOREST-BIOGEOSCIENCES AND FORESTRY LA English DT Article DE Decision-tree Classification; Eastern Hemlock; Hemlock Woolly Adelgid; Remote Sensing; Support Vector Machine ID AIRBORNE SCANNING LIDAR; SUPPORT VECTOR MACHINES; LANDSAT TM DATA; TSUGA-CANADENSIS; MULTISPECTRAL IMAGERY; LASER SCANNER; PINE-BEETLE; NEW-ENGLAND; CLASSIFICATION; FORESTS AB Hemlock woolly adelgid (Adelges tsugae Annand, HWA) is an introduced invasive forest pest in eastern North America. Herbivory by this insect results in mortality to eastern hemlock (Tsuga canadensis L. Carr.) and Carolina hemlock (Tsuga caroliniana Engelm.). These species occur in landscapes where extreme topographic variation is common. The vegetation communities within these landscapes feature high diversity of tree species, including several other conifer species. Traditional forest inventory procedures and insect pest detection methods within these limited-access landscapes are impractical. However, further information is needed to evaluate the impacts of HWA-induced hemlock mortality. Accordingly, our goal was to develop a semi-automatic method for mapping patches of coniferous tree species that include the living hemlock component and tree mortality by the HWA using aerial images and LiDAR (light detection and ranging) to increase our understanding of the severity and pattern of hemlock decline. The study was conducted in the Linville River Gorge in the Southern Appalachians of western North Carolina, USA. The mapping task included a two-phase approach: decision-tree and support vector machine classifications. We found that about 2% of the forest canopy surface was covered by dead trees and 43% by coniferous tree species. A large portion of the forest canopy surface (over 55%) was covered by deciduous tree species. The resulting maps provide a means for evaluating the impact of HWA herbivory, since this insect was the only significant coniferous mortality agent present within the study site. C1 [Kantola, Tuula; Coulson, Robert N.; Tchakerian, Maria D.] Texas A&M Univ, Dept Entomol, Knowledge Engn Lab, College Stn, TX 77843 USA. [Lyytikainen-Saarenmaa, Paivi; Holopainen, Markus] Univ Helsinki, Dept Forest Sci, POB 27, FI-00014 Helsinki, Finland. [Streett, Douglas A.] US Forest Serv, USDA, Southern Res Stn, Alexandria Forestry Ctr, 2500 Shreveport Highway, Pineville, LA 71360 USA. RP Kantola, T (reprint author), Texas A&M Univ, Dept Entomol, Knowledge Engn Lab, College Stn, TX 77843 USA. EM tuula.kantola@helsinki.fi OI Kantola, Tuula/0000-0002-1683-016X FU Graduate School in Forest Sciences (GSForest) in Finland; Finnish Academy project "Centre of Excellence in Laser Scanning Research" (CoE-LaSR) [272195]; University of Helsinki project "Towards semi-supervised characterization and large-area planning of forest resources using airborne laser scanning data acquired for digital elevation modeling"; US Forest Service through USDA Forest Service [SRS-12-CA-11330129-077] FX This study was made possible by financial aid from the Graduate School in Forest Sciences (GSForest) in Finland, the Finnish Academy project "Centre of Excellence in Laser Scanning Research" (CoE-LaSR, decision number 272195), the University of Helsinki project "Towards semi-supervised characterization and large-area planning of forest resources using airborne laser scanning data acquired for digital elevation modeling", and by the US Forest Service through USDA Forest Service cooperative agreement SRS-12-CA-11330129-077. We thank Dr. Hannu Saarenmaa for fruitful insights. NR 74 TC 1 Z9 1 U1 13 U2 18 PU SISEF-SOC ITALIANA SELVICOLTURA ECOL FORESTALE PI POTENZA PA DEPT PROD VEGETALE, VIA ATENEO LUCANO 10, POTENZA, 85100, ITALY SN 1971-7458 J9 IFOREST JI iForest PD APR PY 2016 VL 9 BP 178 EP 186 DI 10.3832/ifor1712-008 PG 9 WC Forestry SC Forestry GA DO9MD UT WOS:000378109800001 ER PT J AU Cosh, MH Ochsner, TE McKee, L Dong, JN Basara, JB Evett, SR Hatch, CE Small, EE Steele-Dunne, SC Zreda, M Sayde, C AF Cosh, Michael H. Ochsner, Tyson E. McKee, Lynn Dong, Jingnuo Basara, Jeffrey B. Evett, Steven R. Hatch, Christine E. Small, Eric E. Steele-Dunne, Susan C. Zreda, Marek Sayde, Chadi TI The Soil Moisture Active Passive Marena, Oklahoma, In Situ Sensor Testbed (SMAP-MOISST): Testbed Design and Evaluation of In Situ Sensors SO VADOSE ZONE JOURNAL LA English DT Article ID WATER-CONTENT SENSORS; DOMAIN REFLECTOMETRY MEASUREMENTS; TACQ COMPUTER-PROGRAM; COSMIC-RAY NEUTRONS; STANDARDIZING CHARACTERIZATION; TIME; CALIBRATION; NETWORK; MESONET AB In situ soil moisture monitoring networks are critical to the development of soil moisture remote sensing missions as well as agricultural and environmental management, weather forecasting, and many other endeavors. These in situ networks utilize a variety of sensors and installation practices, which confounds the development of a unified reference database for satellite calibration and validation programs. As part of the Soil Moisture Active Passive Mission, the Marena, Oklahoma, In Situ Sensor Testbed (SMAP-MOISST) was initiated to perform inter-comparisons and study sensor limitations. Soil moisture sensors that are deployed in major monitoring networks were included in the study, along with new and emerging technologies, such as the Cosmic Ray Soil Moisture Observing System (COSMOS), passive/active distributed temperature sensing (DTS), and global positioning system reflectometers (GPSR). Four profile stations were installed in May of 2010, and soil moisture was monitored to a depth of 1 m on an hourly basis. The four stations were distributed within a circular domain of approximately 600 m diameter, adequate to encompass the sensing range of COSMOS. The sensors included in the base station configuration included the Stevens Water Hydra Probe, Campbell Scientific 616 and 229, Decagon EC-TM, Delta-T Theta Probe, Acclima, and Sentek EnviroSMART capacitance system. In addition, the Pico TRIME system and additional time-domain reflectometry (TDR) systems were deployed when available. It was necessary to apply site-specific calibration to most sensors to reach an RMSE below 0.04 m(3) m(-3). For most sensor types, a single near surface sensor could be scaled to represent the areal-average of a field domain by simple linear regression, resulting in RMSE values around 0.03 m(3) m(-3). C1 [Cosh, Michael H.; McKee, Lynn] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Ochsner, Tyson E.; Dong, Jingnuo] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Basara, Jeffrey B.] Univ Oklahoma, Sch Meteorol, Oklahoma Climatol Survey, Norman, OK 73019 USA. [Evett, Steven R.] USDA ARS, Crop Prod Res Lab, Washington, DC 20250 USA. [Hatch, Christine E.] Univ Massachusetts, Dept Geosci, Amherst, MA 01003 USA. [Small, Eric E.] Univ Colorado Boulder, Dept Geol Sci, Boulder, CO USA. [Steele-Dunne, Susan C.] Delft Univ Technol, Fac Civil Engn & Geosci, NL-2600 AA Delft, Netherlands. [Zreda, Marek] Univ Arizona, Dept Hydrol & Water Resources, Tucson, AZ 85721 USA. [Sayde, Chadi] Oregon State Univ, Dept Biol & Ecol Engn, Corvallis, OR 97331 USA. RP Cosh, MH (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. EM Michael.cosh@ars.usda.gov FU USDA Agricultural Research Service; Oklahoma Agricultural Experiment Station; NASA Terrestrial Hydrology Program [NNH10ZDA001N-THP] FX This project is a result of close collaborative research funded in part by the USDA Agricultural Research Service and the Oklahoma Agricultural Experiment Station. The authors thank Chris Stansberry and the crew at the OSU Range Research Station for outstanding field support. The authors appreciate the contributions from CTEMPS with regard to the installation of the DTS systems. Funding was also provided by NASA Terrestrial Hydrology Program NNH10ZDA001N-THP. NR 46 TC 5 Z9 5 U1 7 U2 11 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD APR PY 2016 VL 15 IS 4 DI 10.2136/vzj2015.09.0122 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA DP2PH UT WOS:000378330800004 ER PT J AU Rathnaiah, G Bannantine, JP Bayles, DO Zinniel, DK Stabel, JR Grohn, YT Barletta, RG AF Rathnaiah, Govardhan Bannantine, John P. Bayles, Darrell O. Zinniel, Denise K. Stabel, Judith R. Grohn, Yrjo T. Barletta, Raul G. TI Analysis of Mycobacterium avium subsp paratuberculosis mutant libraries reveals loci-dependent transposition biases and strategies for novel mutant discovery SO MICROBIOLOGY-SGM LA English DT Article ID GROWTH IN-VITRO; VIRULENCE DETERMINANTS; ESSENTIAL GENES; JOHNES-DISEASE; TUBERCULOSIS; IDENTIFICATION; MUTAGENESIS; GENERATION; VIVO; RESUSCITATION AB Mycobacterium avium subsp. paratuberculosis (MAP), the aetiological agent of Johne's disease, is one of the most important bacterial pathogens in ruminants. A thorough understanding of MAP pathogenesis is needed to develop new vaccines and diagnostic tests. The generation of comprehensive random transposon mutant libraries is a fundamental genetic technology to determine the role of genes in physiology and pathogenesis. In this study, whole MAP genome analysis compared the insertion sites for the mycobacterial transposon Tn5367 derived from the Mycobacterium smegmatis insertion sequence IS1096 and the mariner transposon MycoMarT7 carrying the Himar1 transposase. We determined that only MycoMarT7 provides a random representation of insertions in 99 % of all MAP genes. Analysis of the MAP K-10 genome indicated that 710 of all ORFs do not possess IS1096 recognition sites, while only 37 do not have the recognition site for MycoMarT7. Thus, a significant number of MAP genes remain underrepresented in insertion libraries from IS 1096-derived transposons. Analysis of MycoMarT7 and Tn5367 mutants showed that Tn5367 has a predilection to insert within intergenic regions, suggesting that MycoMarT7 is the more adequate for generating a comprehensive library. However, we uncovered the novel finding that both transposons have loci-dependent biases, with Tn5367 being the most skewed. These loci-dependent transposition biases led to an underestimation of the number of independent mutants required to generate a comprehensive mutant library, leading to an overestimation of essential genes. Herein, we also demonstrated a useful platform for gene discovery and analysis by isolating three novel mutants for each transposon. C1 [Rathnaiah, Govardhan; Zinniel, Denise K.; Barletta, Raul G.] Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE USA. [Bannantine, John P.; Bayles, Darrell O.; Stabel, Judith R.] ARS, Infect Bacterial Dis, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Grohn, Yrjo T.] Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. RP Barletta, RG (reprint author), Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE USA. EM rbarletta@unl.edu OI Bannantine, John/0000-0002-5692-7898 FU United States Department of Agriculture National Institute of Food and Agriculture [2013-67015-21239]; Hatch/Multi State Project [NEB 39-168]; USDA Agricultural Research Service FX This study was supported by the United States Department of Agriculture National Institute of Food and Agriculture award number 2013-67015-21239, the Hatch/Multi State Project (NEB 39-168), and the USDA Agricultural Research Service. We thank Dr Christopher M. Sassetti (Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA) for providing the MycoMarT7 phage and helpful advice. We thank Philion L. Hoff for help in some of the statistical analyses. NR 36 TC 0 Z9 0 U1 1 U2 2 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1350-0872 EI 1465-2080 J9 MICROBIOL-SGM JI Microbiology-(UK) PD APR PY 2016 VL 162 BP 633 EP 641 DI 10.1099/mic.0.000258 PN 4 PG 9 WC Microbiology SC Microbiology GA DO5TZ UT WOS:000377847000004 ER PT J AU Kim, WH Lillehoj, HS Gay, CG AF Kim, W. H. Lillehoj, H. S. Gay, C. G. TI Using genomics to identify novel antimicrobials SO REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES LA English DT Article DE Antibiotic alternative; Antimicrobial peptide; Avian species; Genomics; Granulysin; NK-2; NK-lysin ID CHICKEN NK-LYSIN; GENE-EXPRESSION; T-CELLS; ANTIBACTERIAL ACTIVITY; CYTOLYTIC MOLECULE; EIMERIA-MAXIMA; GRANULYSIN; PEPTIDE; INFECTION; CYTOTOXICITY AB There is a critical need in animal agriculture to develop novel antimicrobials and alternative strategies that will help to reduce the use of antibiotics and address the challenges of antimicrobial resistance. High-throughput gene expression analysis is providing new tools that are enabling the discovery of host-derived antimicrobial peptides. Examples of gene-encoded natural antibiotics that have gained attention include antimicrobial peptides such as human granulysin and its multi-species homolog, namely NK-lysin, which provide a protective response against a broad range of microbes and are a principal component of innate immunity in vertebrates. Both granulysin and NK-lysin are localised in cytolytic granules in natural killer and cytotoxic T lymphocytes. Host-derived NK-lysins that were first described in mammals are also found in avian species, and they have been shown to have antimicrobial activities that could potentially be used to control important poultry pathogens. Morphological alterations observed following chicken NK-lysin binding to Eimeria sporozoites and Escherichia coli membranes indicate damage and disruption of cell membranes, suggesting that NK-lysin kills pathogenic protozoans and bacteria by direct interaction. Genotype analysis revealed that chicken NK-lysin peptides derived from certain alleles were more effective at killing pathogens than those derived from others, which could potentially affect susceptibility to diseases. Although the host-derived antimicrobial peptides described in this paper may not, by themselves, be able to replace the antibiotics currently used in animal production, their use as specific treatments based on their known mechanisms of action is showing promising results. C1 [Kim, W. H.; Lillehoj, H. S.] USDA, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Gay, C. G.] ARS, Anim Prod & Protect Off, Natl Programs, George Washington Carver Ctr,USDA, 5601 Sunnyside Ave, Beltsville, MD 20705 USA. RP Gay, CG (reprint author), ARS, Anim Prod & Protect Off, Natl Programs, George Washington Carver Ctr,USDA, 5601 Sunnyside Ave, Beltsville, MD 20705 USA. EM cyril.gay@ars.usda.gov NR 51 TC 0 Z9 0 U1 10 U2 12 PU OFFICE INT EPIZOOTIES PI PARIS PA 12 RUE DE PRONY, 75017 PARIS, FRANCE SN 0253-1933 J9 REV SCI TECH OIE JI Rev. Sci. Tech. Off. Int. Epizoot. PD APR PY 2016 VL 35 IS 1 BP 95 EP 103 DI 10.20506/rst.35.1.2420 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA DO8EA UT WOS:000378014200008 PM 27217171 ER PT J AU Kao, RR Price-Carter, M Robbe-Austerman, S AF Kao, R. R. Price-Carter, M. Robbe-Austerman, S. TI y Use of genomics to track bovine tuberculosis transmission SO REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES LA English DT Article DE Bovine tuberculosis; Contact tracing; Genomics; High-throughput sequencing; Multi-host pathogen; Phylodynamics ID TANDEM-REPEAT ANALYSIS; RESTRICTION-ENDONUCLEASE ANALYSIS; POSSUMS TRICHOSURUS-VULPECULA; TB INFECTION STATUS; MYCOBACTERIUM-BOVIS; VARIABLE-NUMBER; MOLECULAR EPIDEMIOLOGY; GREAT-BRITAIN; STRAIN DIFFERENTIATION; NEW-ZEALAND AB The control of any infectious disease of livestock is made more difficult by the presence of a wildlife reservoir, as the reservoir is often poorly observed and difficult to manage. This problem is particularly acute for bovine tuberculosis (bTB) because the long duration of infection and low levels of infectiousness make tracing the sources of infection difficult. For over 30 years, the process of contact tracing has been aided by the exploitation of molecular markers in the pathogen, but this has largely only been capable of characterising broad associations between large communities of similar types. However, the recent advent of mass high-throughput 'whole-genome' sequencing (WGS) has revolutionised forensic epidemiology for other diseases, and now it has the potential to do so for bTB. In this review, the authors consider the historical context of WGS use and look at what prior molecular techniques have already achieved. They outline the key approaches to interpreting WGS data and consider both the role of advanced analytical techniques that exploit the evolutionary and epidemiological properties of the system and the problems associated with quantifying the role of hidden reservoirs of disease. Finally, they consider the particular difficulties associated with developing this technology for routine diagnostics and its potential for mass use. C1 [Kao, R. R.] Univ Glasgow, Coll Med Vet & Life Sci, Inst Biodivers Anim Hlth & Comparat Med, Glasgow G61 1QH, Lanark, Scotland. [Price-Carter, M.] AgResearch, Tennent Dr,Private Bag 11008, Palmerston North 4442, New Zealand. Anim & Plant Hlth Inspect Serv, USDA, Natl Vet Serv Labs, Ames, IA USA. RP Kao, RR (reprint author), Univ Glasgow, Coll Med Vet & Life Sci, Inst Biodivers Anim Hlth & Comparat Med, Glasgow G61 1QH, Lanark, Scotland. EM rowland.kao@glasgow.ac.uk NR 108 TC 1 Z9 1 U1 11 U2 17 PU OFFICE INT EPIZOOTIES PI PARIS PA 12 RUE DE PRONY, 75017 PARIS, FRANCE SN 0253-1933 J9 REV SCI TECH OIE JI Rev. Sci. Tech. Off. Int. Epizoot. PD APR PY 2016 VL 35 IS 1 BP 241 EP 258 DI 10.20506/rst.35.1.2430 PG 18 WC Veterinary Sciences SC Veterinary Sciences GA DO8EA UT WOS:000378014200018 PM 27217181 ER PT J AU Lati, RN Mou, BQ Rachuy, JS Fennimore, SA AF Lati, Ran N. Mou, Beiquan Rachuy, John S. Fennimore, Steven A. TI Evaluation of Cycloate Followed by Evening Two-Leaf-Stage Phenmedipham Application in Fresh Market Spinach SO WEED TECHNOLOGY LA English DT Article DE Evening application; morning application; sequential phenmedipham application; time of day; two-leaf application ID WEED-CONTROL; SUGAR-BEET; HERBICIDES; TIME AB Fresh market spinach has one primary herbicide, cycloate, which does not control all weeds. Previous studies demonstrated that cycloate PRE followed by (fb) phenmedipham at the four-leaf spinach stage is a safe and effective treatment. However, this treatment is not useful for the main growing season of fresh spinach due to its short crop cycle and the 21-d preharvest interval requirement of phenmedipham. This study evaluates the potential to use the combination of cycloate PRE fb phenmedipham on two-leaf spinach. Greenhouse and field studies were conducted in 2014 using three spinach varieties with low ('Nordic' and 'Sardinia') and high ('Regal') tolerance to phenmedipham. Greenhouse studies revealed that phenmedipham at 90 g ai ha(-1) was safe to Regal when applied at the two-leaf stage. Sardinia was more susceptible to phenmedipham injury under high (310 W m(-2)) light conditions than low (258 W m(-2)) light conditions. Impact of time of day on phenmedipham safety was evaluated in the field: day-long exposure to high light intensity following morning applications vs. evening applications fb exposure to low light intensity. Injury estimations taken 3 d after treatment (DAT) were lower for evening than for morning applications. Nonetheless, injury 11 DAT and spinach yield evaluations found no differences between morning and evening applications. Subsequently, cycloate (1,700 g ha(-1)) PRE fb phenmedipham (90 and 180 g ha(-1)) applied in the evening at the two-leaf stage was evaluated. A reference treatment was cycloate PRE fb phenmedipham (270 g ha(-1)) at the four-leaf stage. Treatments with cycloate fb two-leaf phenmedipham at 90 and 180 g ha(-1) were safe to spinach and improved weed control compared to cycloate alone. Cycloate fb 180 g ha(-1) phenmedipham at the two-leaf stage reduced weed biomass by 88% compared to cycloate alone. This level of weed control was similar to the reference treatment. Results here show that phenmedipham applied at the two-leaf stage is safe to fresh market spinach and it has the potential to be used during most of the fresh spinach growing season. Nomenclature: Cycloate; phenmedipham; spinach, Spinacia oleracea L. 'Nordic', 'Regal', 'Sardinia'. C1 [Lati, Ran N.; Rachuy, John S.; Fennimore, Steven A.] Univ Calif Davis, Dept Plant Sci, 1636 East Alisal, Salinas, CA 93905 USA. [Mou, Beiquan] USDA ARS, 1636 East Alisal St, Salinas, CA 93905 USA. RP Fennimore, SA (reprint author), Univ Calif Davis, Dept Plant Sci, 1636 East Alisal, Salinas, CA 93905 USA. EM ranlati@gmail.com NR 23 TC 1 Z9 1 U1 0 U2 0 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2016 VL 30 IS 2 BP 464 EP 471 DI 10.1614/WT-D-15-00102.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DO5CU UT WOS:000377802000014 ER PT J AU Nghiem, NP Senske, GE Kim, TH AF Nghiem, Nhuan P. Senske, Gerard E. Kim, Tae Hyun TI Pretreatment of Corn Stover by Low Moisture Anhydrous Ammonia (LMAA) in a Pilot-Scale Reactor and Bioconversion to Fuel Ethanol and Industrial Chemicals SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE Cellulosic biomass; Corn stover; Pretreatment; Low moisture anhydrous ammonia; Ethanol; Value-added products; Fermentation ID SUCCINIC ACID; FERMENTATION; HYDROLYSATE; FRACTIONATION; SOFTWOOD; LIGNIN; STRAW AB Corn stover (CS) adjusted to 50, 66, and 70 % moisture was pretreated by the low moisture anhydrous ammonia (LMAA) process in a pilot-scale ammoniation reactor. After ammoniation, the 70 % moisture CS was treated at 90 and 100 A degrees C whereas the others were treated at 90 A degrees C only. The 70 % moisture pretreated CS then was subjected to a storage study under non-sterile conditions for 3 months. It was found that storage time did not have significant effects on the compositions of the pretreated materials and their hydrolysis by commercial enzymes. The 70 % moisture CS treated at 90 A degrees C was used for preparation of a mix sugar hydrolysate (MSH) using combination of cellulase and xylanase. The MSH was used to prepare a corn mash at 9.5 wt% solid then subjected to ethanol fermentation by Escherichia coli KO11. The 66 % moisture CS treated at 90 A degrees C was hydrolyzed with xylanase to make a xylose-rich hydrolysate (XRH), which was subsequently used for butyric acid fermentation by Clostridium tyrobutyricum. The resultant cellulose-enriched residue was hydrolyzed with cellulase to make a glucose-rich hydrolysate (GRH), which was subsequently used for succinic acid fermentation by E. coli AFP184. C1 [Nghiem, Nhuan P.; Senske, Gerard E.] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Kim, Tae Hyun] Kongju Natl Univ, Dept Environm Engn, Cheonandae Ro 1223-24, Cheonan 31080, Chungnam, South Korea. RP Nghiem, NP (reprint author), ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM john.nghiem@ars.usda.gov NR 23 TC 0 Z9 0 U1 6 U2 9 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 EI 1559-0291 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD APR PY 2016 VL 179 IS 1 BP 111 EP 125 DI 10.1007/s12010-016-1982-2 PG 15 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DO0JJ UT WOS:000377464900008 PM 26769706 ER PT J AU Bondi, CA Beier, CM Ducey, PK Lawrence, GB Bailey, S AF Bondi, Cheryl A. Beier, Colin M. Ducey, Peter K. Lawrence, Gregory B. Bailey, Scott TI Can the eastern red-backed salamander (Plethodon cinereus) persist in an acidified landscape? SO ECOSPHERE LA English DT Article DE acidic deposition; generalist; local adaptation; northern hardwood forests; Plethodon cinereus; soil acidity; woodland salamanders ID NORTHEASTERN UNITED-STATES; TERRESTRIAL SALAMANDERS; LOW PH; LOCAL ADAPTATION; NEW-YORK; ADIRONDACK MOUNTAINS; AMBYSTOMA-MACULATUM; EMBRYONIC MORTALITY; SPECIES COMPOSITION; ACID PRECIPITATION AB Hardwood forests of eastern North America have experienced decades of acidic deposition, leading to soil acidification where base cation supply was insufficient to neutralize acid inputs. Negative impacts of soil acidity on amphibians include disrupted embryonic development, lower growth rates, and habitat loss. However, some amphibians exhibit intraspecific variation in acid tolerance, suggesting the potential for local adaptation in areas where soils are naturally acidic. The eastern red-backed salamander (Plethodon cinereus) is a highly abundant top predator of the northern hardwood forest floor. Early research found that P. cinereus was sensitive to acidic soils, avoiding substrates with pH < 3.8 and experiencing decreased growth rates in acidic habitats. However, recent studies have documented P. cinereus populations in lower pH conditions than previously observed, suggesting some populations may persist in acidic conditions. Here, we evaluated relationships between organic horizon soil pH and P. cinereus abundance, adult health (body size and condition), and microhabitat selection, based on surveys of 34 hardwood forests in northeastern United States that encompass a regional soil pH gradient. We found no associations between soil pH and P. cinereus abundance or health, and observed that this salamander used substrates with pH similar to that available, suggesting that pH does not mediate their fine-scale distributions. The strongest negative predictor of P. cinereus abundance was the presence of dusky salamanders (Desmognathus spp.), which were most abundant in the western Adirondacks. Our results indicate that P. cinereus occupies a wider range of soil pH than has been previously thought, which has implications for their functional role in forest food webs and nutrient cycles in acid-impaired ecosystems. Tolerance of P. cinereus for more acidic habitats, including anthropogenically acidified forests, may be due to local adaptation in reproductively isolated populations and/or generalist life history traits that allow them to exploit a wider resource niche. C1 [Bondi, Cheryl A.; Beier, Colin M.] SUNY Coll Environm Sci & Forestry, Grad Program Environm Sci, Syracuse, NY 13210 USA. [Beier, Colin M.] SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. [Ducey, Peter K.] SUNY Coll Cortland, Biol Sci Dept, Cortland, NY 13045 USA. [Lawrence, Gregory B.] US Geol Survey, New York Water Sci Ctr, Troy, NY 12180 USA. [Bailey, Scott] US Forest Serv, USDA, No Res Stn, North Woodstock, NH 03262 USA. RP Beier, CM (reprint author), SUNY Coll Environm Sci & Forestry, Grad Program Environm Sci, Syracuse, NY 13210 USA.; Beier, CM (reprint author), SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. EM cbeier@esf.edu OI Bailey, Scott/0000-0002-9160-156X FU U.S. Forest Service Northeastern States Research Cooperative; National Science Foundation FX This research was supported by a grant from the U.S. Forest Service Northeastern States Research Cooperative (www.nsrc.org). We are grateful to Chelsea Geyer, Jamie Wahls, Amanda Temple, Drew Smith, and Sabrina Green for field and laboratory assistance, and to Blair Page, Steve Horsley, Robert Long, and Richard Hallett for sharing data from their research sites. Plots from Bartlett Experimental Forest (USDA Forest Service Northern Research Station) were established as part of a larger project on Multiple Element Limitation in Northern Hardwood Ecosystems (http://www.esf.edu/melnhe/), funded by the National Science Foundation. We thank the staff of the Green Mountain National Forest, White Mountain National Forest, and the Equinox Preservation Trust, for their cooperation in providing access to their lands. We obtained Scientific Collecting permits from New Hampshire Fish and Game, Vermont Fish and Wildlife, and New York Department of Environmental Conservation to handle salamanders, and followed methods approved by the Institutional Animal Care and Use Committee of the State University of New York, College of Environmental Science and Forestry (IACUC #120403). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 70 TC 0 Z9 0 U1 12 U2 17 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2016 VL 7 IS 4 AR e01318 DI 10.1002/ecs2.1318 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DN6VA UT WOS:000377213400012 ER PT J AU Gilliam, FS Welch, NT Phillips, AH Billmyer, JH Peterjohn, WT Fowler, ZK Walter, CA Burnham, MB May, JD Adams, MB AF Gilliam, Frank S. Welch, Nicole Turrill Phillips, Anne Hockenberry Billmyer, Jake H. Peterjohn, William T. Fowler, Zachariah K. Walter, Christopher A. Burnham, Mark B. May, Jeffrey D. Adams, Mary Beth TI Twenty-five-year response of the herbaceous layer of a temperate hardwood forest to elevated nitrogen deposition SO ECOSPHERE LA English DT Article DE eastern deciduous forest; forest ecosystems; forest strata; herbaceous layer; nitrogen saturation ID ATMOSPHERIC REACTIVE NITROGEN; PLANT-SPECIES DIVERSITY; TERRESTRIAL ECOSYSTEMS; UNITED-STATES; CLEAR-CUT; ENVIRONMENTAL HETEROGENEITY; UNDERSTORY VEGETATION; SOIL HETEROGENEITY; GLOBAL ASSESSMENT; FLOOR VEGETATION AB Increasing rates of atmospheric deposition of nitrogen (N) present a novel threat to the biodiversity of terrestrial ecosystems. Many forests are particularly susceptible to excess N given their proximity to sources of anthropogenic N emissions. This study summarizes results of a 25-yr treatment of an entire central Appalachian hardwood forest watershed via aerial applications of N with a focus on effects of added N on the cover, species richness, and composition of the herbaceous layer. Research was carried out on two watersheds of the Fernow Experimental Forest (FEF), West Virginia. The long-term reference watershed at FEF (WS4) was used as a reference; WS3 was experimentally treated, receiving three aerial applications of N per year as (NH4)(2)SO4 totaling 35 kg N ha(-1) yr(-1), beginning in 1989. Cover of the herbaceous layer (vascular plants <= 1 m in height) was estimated visually in five circular 1-m(2) subplots within each of seven circular 400-m(2) sample plots spanning all aspects and elevations of each watershed. Sampling was carried out in early July of each of the following years: 1991, 1992, 1994, 2003, and 2009-2014, yielding 10 yr of data collected over a 23-yr period. It was anticipated that the N treatment on WS3 would decrease species richness and alter herb layer composition by enhancing cover of a few nitrophilic species at the expense of numerous N-efficient species. Following a period of minimal response from 1991 to 1994, cover of the herb layer increased substantially on N-treated WS3, and remained high thereafter. There was also a coincidental decrease in herb layer diversity during this period, along with a sharp divergence in community composition between WS4 and WS3. Most changes appear to have arisen from unprecedented, N-mediated increases of Rubus spp., which are normally associated with the high-light environment of openings, rather than beneath intact forest canopies. These findings support the prediction that N-mediated changes in the herbaceous layer of impacted forests are driven primarily by increases in nitrophilic species. C1 [Gilliam, Frank S.; Billmyer, Jake H.; May, Jeffrey D.] Marshall Univ, Dept Biol Sci, Huntington, WV 25755 USA. [Welch, Nicole Turrill] Mississippi Univ Women, Dept Sci & Math, Columbus, MS 39701 USA. [Phillips, Anne Hockenberry] 738 Eagle Mill Court, Marietta, GA 30068 USA. [Peterjohn, William T.; Fowler, Zachariah K.; Walter, Christopher A.; Burnham, Mark B.] W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA. [Adams, Mary Beth] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. RP Gilliam, FS (reprint author), Marshall Univ, Dept Biol Sci, Huntington, WV 25755 USA. EM gilliam@marshall.edu FU United States Department of Agriculture (USDA) Forest Service, Fernow Experimental Forest, Timber and Watershed Laboratory, Parsons, W.V. [23-165, 23-590, 23-842]; USDA National Research Initiative Competitive Grants (Grant NRICGP) [2006-35101-17097]; Long Term Research in Environmental Biology (LTREB) program at the National Science Foundation [DEB-0417678, DEB-1019522] FX Research was funded through United States Department of Agriculture (USDA) Forest Service, Fernow Experimental Forest, Timber and Watershed Laboratory, Parsons, W.V., under USDA Forest Service Cooperative Grants 23-165, 23-590, and 23-842. Additional funding for this research was provided by USDA National Research Initiative Competitive Grants (Grant NRICGP #2006-35101-17097) and by the Long Term Research in Environmental Biology (LTREB) program at the National Science Foundation (Grant No. DEB-0417678 and DEB-1019522). We acknowledge the excellent field assistance of Staci Joy, Brad Yurish, Mark Fisher, Dave Willson, and the late Bill Grafton, to whose memory this study is dedicated. NR 88 TC 1 Z9 1 U1 9 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2016 VL 7 IS 4 AR e01250 DI 10.1002/ecs2.1250 PG 16 WC Ecology SC Environmental Sciences & Ecology GA DN6VA UT WOS:000377213400004 ER PT J AU Gustafson, EJ De Bruijn, AMG Miranda, BR Sturtevant, BR AF Gustafson, Eric J. De Bruijn, Arjan M. G. Miranda, Brian R. Sturtevant, Brian R. TI Implications of mechanistic modeling of drought effects on growth and competition in forest landscape models SO ECOSPHERE LA English DT Article DE climate change; drought-induced mortality; first principles; forest succession; LANDIS-II; mechanistic landscape model; PnET-Succession; water stress ID CLIMATE-CHANGE; UNITED-STATES; VEGETATION MORTALITY; CARBON ALLOCATION; SOIL-MOISTURE; LANDIS-II; PHOTOSYNTHESIS; SUCCESSION; PROJECTIONS; SIMULATION AB The incidence of drought is expected to increase worldwide as a factor structuring forested landscapes. Ecophysiological mechanisms are being added to Forest Landscape Models (FLMs) to increase their robustness to the novel environmental conditions of the future (including drought), but their behavior has not been evaluated for mixed temperate forests. We evaluated such an approach by assessing the ability of physiological mechanisms to predict susceptibility to tree mortality as a function of drought in the upper Midwest (USA) through controlled site-level drought simulation experiments using the PnET-Succession extension of the LANDIS-II FLM. We also conducted a landscape-level experiment to study landscape response to drought treatments in the presence of the spatial processes of seed dispersal and stand-replacing disturbance. At the site level we found that net photosynthesis and carbon reserves showed a clear negative response to both the length of drought and the alternating pulses of normal precipitation and drought events, with soils holding more water moderating this response. The effect of the drought treatments varied somewhat depending on the assemblage of competitors and their specific life-history traits such as ability to compete for light, maximum photosynthetic capacity and water use efficiency. A large diversity of assemblages were simulated at the landscape level, and species abundance generally sorted by photosynthetic capacity (foliar nitrogen) and life form (deciduous vs. evergreen), with faster growing species and deciduous species suffering less decline because of drought. Soil type also had an impact on total productivity (biomass), with soils having higher available water being more productive through time. We conclude that (1) the mechanistic, first principles approach is advantageous for global change research because the combination of life-history traits of competitors interact to cause a site-specific dynamic response to fundamental drivers (e.g., precipitation, temperature), and these site-level responses interact spatially to create landscape responses that are complex and difficult to project with less mechanistic approaches, and (2) published findings that increasing drought length (rather than severity) increases tree mortality in the upper Midwest are clearly consistent with a mechanism of acute photosynthetic depression resulting in increased likelihood of carbon starvation as droughts lengthen. C1 [Gustafson, Eric J.; De Bruijn, Arjan M. G.; Miranda, Brian R.; Sturtevant, Brian R.] US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, USDA, 5985 Highway K, Rhinelander, WI 54501 USA. [De Bruijn, Arjan M. G.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. RP Gustafson, EJ (reprint author), US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, USDA, 5985 Highway K, Rhinelander, WI 54501 USA. EM egustafson@fs.fed.us FU Northern Research Station of the USDA Forest Service; USDA National Institute of Food and Agriculture Grant [105321] FX Funding was provided by the Northern Research Station of the USDA Forest Service and USDA National Institute of Food and Agriculture Grant #105321. We also thank Robert Scheller, Matthew Duveneck, Steve Shifley, Robert Pangle, and two anonymous reviewers for critical reviews that greatly improved the manuscript. NR 56 TC 0 Z9 0 U1 9 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2016 VL 7 IS 4 AR e01253 DI 10.1002/ecs2.1253 PG 23 WC Ecology SC Environmental Sciences & Ecology GA DN6VA UT WOS:000377213400005 ER PT J AU Jacobson, SL Bliss-Ketchum, LL de Rivera, CE Smith, WP AF Jacobson, Sandra L. Bliss-Ketchum, Leslie L. de Rivera, Catherine E. Smith, Winston P. TI A behavior-based framework for assessing barrier effects to wildlife from vehicle traffic volume SO ECOSPHERE LA English DT Article DE antipredator behavior; barrier effect; habitat connectivity; highways; risk-disturbance; roadkill; traffic flow; traffic intensity; vehicle-caused mortality; wildlife behavior ID ROAD-KILLS; POPULATION PERSISTENCE; SOUTHERN CALIFORNIA; GENETIC DIVERSITY; CENTRAL FLORIDA; GRIZZLY BEARS; MORTALITY; DENSITY; PERMEABILITY; HERPETOFAUNA AB Roads, while central to the function of human society, create barriers to animal movement through collisions and habitat fragmentation. Barriers to animal movement affect the evolution and trajectory of populations. Investigators have attempted to use traffic volume, the number of vehicles passing a point on a road segment, to predict effects to wildlife populations approximately linearly and along taxonomic lines; however, taxonomic groupings cannot provide sound predictions because closely related species often respond differently. We assess the role of wildlife behavioral responses to traffic volume as a tool to predict barrier effects from vehicle-caused mortality and avoidance, to provide an early warning system that recognizes traffic volume as a trigger for mitigation, and to better interpret roadkill data. We propose four categories of behavioral response based on the perceived danger to traffic: Nonresponders, Pausers, Speeders, and Avoiders. Nonresponders attempt to cross highways regardless of traffic volume. Pausers stop in the face of danger so have a low probability of successful crossing when traffic volume increases. Hence, highway barrier effects are primarily due to mortality for Nonresponders and Pausers at high traffic volumes. Speeders run away from danger but are unable to do so successfully as traffic volume increases. At moderate to high volume, Speeders are repelled by traffic danger. Avoiders face lower mortality than other categories because they begin to avoid traffic at relatively low traffic volumes. Hence, avoidance causes barrier effects more than mortality for Speeders and Avoiders even at relatively moderate traffic volumes. By considering a species' risk-avoidance response to traffic, managers can make more appropriate and timely decisions to mitigate effects before populations decline or become locally extinct. C1 [Jacobson, Sandra L.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Bliss-Ketchum, Leslie L.; de Rivera, Catherine E.] Portland State Univ, Sch Environm, Dept Environm Sci & Management, Portland, OR 97207 USA. [Smith, Winston P.] US Forest Serv, USDA, Pacific Northwest Res Stn, La Grande, OR 97850 USA. [Smith, Winston P.] Univ Alaska, Inst Arctic Biol, Juneau, AK 99801 USA. RP Jacobson, SL (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. EM sjacobson@fs.fed.us NR 73 TC 1 Z9 1 U1 19 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2016 VL 7 IS 4 AR e01345 DI 10.1002/ecs2.1345 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DN6VA UT WOS:000377213400018 ER PT J AU Selmants, PC Adair, KL Litton, CM Giardina, CP Schwartz, E AF Selmants, Paul C. Adair, Karen L. Litton, Creighton M. Giardina, Christian P. Schwartz, Egbert TI Increases in mean annual temperature do not alter soil bacterial community structure in tropical montane wet forests SO ECOSPHERE LA English DT Article DE bacteria; climate change; Hawaii; microbial diversity; tropical forests ID MICROBIAL COMMUNITIES; RAIN-FOREST; PHYLOGENETIC DIVERSITY; CARBON STORAGE; SCALE; DECOMPOSITION; CONSERVATION; RESILIENCE; RESISTANCE; CONVERSION AB Soil bacteria play a key role in regulating terrestrial biogeochemical cycling and greenhouse gas fluxes across the soil-atmosphere continuum. Despite their importance to ecosystem functioning, we lack a general understanding of how bacterial communities respond to climate change, especially in relatively understudied ecosystems like tropical montane wet forests. We used a well-studied and highly constrained 5.2 degrees C mean annual temperature (MAT) gradient in tropical montane wet forests on the Island of Hawaii to test the hypothesis that long-term, whole-ecosystem warming and the accompanying increase in belowground carbon flux increase the diversity and alter the composition of soil bacterial communities. Across this MAT gradient, dominant vegetation, substrate type and age, soil moisture, and disturbance history are constant, allowing us to effectively isolate the influence of rising MAT on soil bacterial community structure. Contrary to our hypothesis, we found that the richness, evenness, and phylogenetic diversity of the soil bacterial community remained remarkably stable with MAT and that MAT did not predict variation in bacterial community composition despite a substantial increase in belowground soil carbon fluxes across the gradient. Our results suggest that other factors that are constant across this gradient-such as soil pH, water availability and plant species composition-may be more important than warming in influencing soil bacterial community composition and diversity, at least within the temperature range studied here (similar to 1318 degrees C MAT). Ours is the first study to demonstrate stability of soil bacterial community structure with rising MAT and increased belowground carbon flux in a tropical wet forest ecosystem. Moreover, our results add to growing evidence that the diversity and composition of soil bacterial communities dominated by Proteobacteria and Acidobacteria in low-pH forest soils may be insensitive to the direct effect of climate warming. C1 [Selmants, Paul C.; Litton, Creighton M.] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Adair, Karen L.] Univ Canterbury, Sch Biol Sci, Christchurch 8140, New Zealand. [Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. [Schwartz, Egbert] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86001 USA. RP Selmants, PC (reprint author), Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. EM selmants@hawaii.edu RI Giardina, Christian/C-3120-2011; OI Giardina, Christian/0000-0002-3431-5073; Selmants, Paul/0000-0001-6211-3957 FU National Science Foundation [EF-0747397, DEB-0816486]; USDA Forest Service Institute of Pacific Islands Forestry, Pacific Southwest Research Station [09-JV-11272177-029, 12-JV-11272139-047]; College of Tropical Agriculture and Human Resources at the University of Hawaii at Manoa via the USDA National Institute of Food and Agriculture [HAW00132-H, HAW01127-H, HAW00188-M, HAW01123-M]; College of Tropical Agriculture and Human Resources at the University of Hawaii at Manoa via the Hatch [HAW00132-H, HAW01127-H, HAW00188-M, HAW01123-M]; College of Tropical Agriculture and Human Resources at the University of Hawaii at Manoa via the McIntire-Stennis Programs [HAW00132-H, HAW01127-H, HAW00188-M, HAW01123-M] FX We thank the USDA Forest Service and State of Hawaii Department of Land and Natural Resources Division of Forestry and Wildlife for access to the Hawaii Experimental Tropical Forest and the US Fish and Wildlife Service for access to the Hakalau Forest National Wildlife Refuge. Thanks to Devin Leopold and two anonymous reviewers for providing valuable comments on previous versions of this article. This study was funded by the National Science Foundation CAREER Program ( grant no. EF-0747397 awarded to ES), the National Science Foundation Ecosystem Science Program ( grant no. DEB-0816486 awarded to CML and CPG), the USDA Forest Service Institute of Pacific Islands Forestry, Pacific Southwest Research Station ( Research Joint Ventures 09-JV-11272177-029 and 12-JV-11272139-047 awarded to CPG), and the College of Tropical Agriculture and Human Resources at the University of Hawaii at Manoa via the USDA National Institute of Food and Agriculture, Hatch and McIntire-Stennis Programs ( HAW00132-H, HAW01127-H, HAW00188-M, and HAW01123-M awarded to CML). NR 61 TC 0 Z9 0 U1 14 U2 20 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2016 VL 7 IS 4 AR e01296 DI 10.1002/ecs2.1296 PG 10 WC Ecology SC Environmental Sciences & Ecology GA DN6VA UT WOS:000377213400009 ER PT J AU Herriman, KR Davis, AS Apostol, KG Kildisheva, OA Ross-Davis, AL Dumroese, RK AF Herriman, Kayla R. Davis, Anthony S. Apostol, Kent G. Kildisheva, Olga. A. Ross-Davis, Amy L. Dumroese, R. Kasten TI Do Container Volume, Site Preparation, and Field Fertilization Affect Restoration Potential of Wyoming Big Sagebrush? SO NATURAL AREAS JOURNAL LA English DT Article DE arid ecosystems; Artemisia tridentata ssp wyomingensis; container stock; Great Basin; seedling size ID SEEDLINGS; PERFORMANCE; PLANTS; FIRE; MORPHOLOGY; DROUGHT; STEPPE; ROOT AB Land management practices, invasive species expansion, and changes in the fire regime greatly impact the distribution of native plants in natural areas. Wyoming big sagebrush (Artemisia tridentata ssp. wyomingensis), a keystone species in the Great Basin, has seen a 50% reduction in its distribution. For many dryland species, reestablishment efforts have focused on direct seeding but achieved only minor success due to irregular seed germination and poor survival. We investigated the effects of container volume and fertilization on the performance of nursery-grown Wyoming big sagebrush seedlings following outplanting. Additionally, to evaluate the impact of site preparation, one-year-old seedlings were outplanted into tilled and untilled field conditions. Initial seedling size increased with container volume; larger seedlings exhibited significantly higher root collar diameter (RCD) growth than smaller seedlings. As container volume increased from 108 to 251 and 336 cm(3), seedling survival on the tilled plot increased from 28 to 44 and 43%, respectively, whereas no influence was observed on the untilled plot (57 to 61, and 60%, respectively). Fertilization reduced seedling survival on tilled (by 25%) and untilled (by 14%) plots. Fertilizer increased RCD growth on the untilled plot but final nutrient status was unaffected. Thus, container volume may influence seedling morphology and optimize establishment, while field fertilization, especially during spring outplanting when planting sites have low moisture availability, may hinder first-year survival. C1 [Herriman, Kayla R.; Davis, Anthony S.; Apostol, Kent G.; Kildisheva, Olga. A.] Univ Idaho, Ctr Forest Nursery & Seedling Res, Coll Nat Resources, POB 441133, Moscow, ID 83843 USA. [Ross-Davis, Amy L.; Dumroese, R. Kasten] US Forest Serv, USDA, Rocky Mt Res Stn, 1221 South Main St, Moscow, ID 83843 USA. RP Davis, AS (reprint author), Univ Idaho, Ctr Forest Nursery & Seedling Res, Coll Nat Resources, POB 441133, Moscow, ID 83843 USA. EM asdavis@uidaho.edu FU USDA Forest Service; Great Basin Restoration Initiative; University of Idaho Center for Forest Nursery and Seedling Research; Rocky Mountain Research Station; Great Basin Native Plant Selection and Increase Project; USDI Bureau of Land Management FX This study was funded by the USDA Forest Service, Rocky Mountain Research Station, Great Basin Native Plant Selection and Increase Project, the USDI Bureau of Land Management, Great Basin Restoration Initiative, and the University of Idaho Center for Forest Nursery and Seedling Research. We thank Heather Gang, Rob Keefe, Kiana Muhs, Jeremiah Pinto, Nathan Robertson, Nancy Shaw, Karen Sjoquist, and Margaret Ward for field support. NR 32 TC 1 Z9 1 U1 7 U2 8 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD APR PY 2016 VL 36 IS 2 BP 194 EP 201 PG 8 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA DO0OA UT WOS:000377477000011 ER PT J AU Abrams, MD Nowacki, GJ AF Abrams, Marc D. Nowacki, Gregory J. TI An interdisciplinary approach to better assess global change impacts and drought vulnerability on forest dynamics SO TREE PHYSIOLOGY LA English DT Editorial Material ID EASTERN UNITED-STATES; EUROPEAN VEGETATION CHANGE; PHOTOSYNTHETIC CAPACITY; FIELD EXPERIMENTS; IMPORTANT DRIVER; CLIMATE-CHANGE; NORTH-AMERICA; US FORESTS; RESPONSES; HISTORY C1 [Abrams, Marc D.] Penn State Univ, Dept Ecosyst Sci & Management, 307 Forest Resources Bldg, University Pk, PA 16802 USA. [Nowacki, Gregory J.] US Forest Serv, USDA, Eastern Reg Off, 626 E Wisconsin Ave, Milwaukee, WI 53202 USA. RP Abrams, MD (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, 307 Forest Resources Bldg, University Pk, PA 16802 USA. EM agl@psu.edu NR 68 TC 1 Z9 1 U1 6 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD APR PY 2016 VL 36 IS 4 BP 421 EP 427 DI 10.1093/treephys/tpw005 PG 7 WC Forestry SC Forestry GA DN9MP UT WOS:000377404000003 PM 26941289 ER PT J AU Gaines, KP Stanley, JW Meinzer, FC McCulloh, KA Woodruff, DR Chen, W Adams, TS Lin, H Eissenstat, DM AF Gaines, Katie P. Stanley, Jane W. Meinzer, Frederick C. McCulloh, Katherine A. Woodruff, David R. Chen, Weile Adams, Thomas S. Lin, Henry Eissenstat, David M. TI Reliance on shallow soil water in a mixed-hardwood forest in central Pennsylvania SO TREE PHYSIOLOGY LA English DT Article DE Critical Zone Observatory; ecohydrology; ecophysiology; root ecology; rooting depth; sap flux; stable isotopes; tree water use ID STABLE-ISOTOPE; HYDRAULIC LIFT; ROOT DISTRIBUTIONS; MODEL SIMULATIONS; TREE ROOTS; DEEP ROOTS; VEGETATION; PLANTS; DYNAMICS; CLIMATE AB We investigated depth of water uptake of trees on shale-derived soils in order to assess the importance of roots over a meter deep as a driver of water use in a central Pennsylvania catchment. This information is not only needed to improve basic understanding of water use in these forests but also to improve descriptions of root function at depth in hydrologic process models. The study took place at the Susquehanna Shale Hills Critical Zone Observatory in central Pennsylvania. We asked two main questions: (i) Do trees in a mixed-hardwood, humid temperate forest in a central Pennsylvania catchment rely on deep roots for water during dry portions of the growing season? (ii) What is the role of tree genus, size, soil depth and hillslope position on the depth of water extraction by trees? Based on multiple lines of evidence, including stable isotope natural abundance, sap flux and soil moisture depletion patterns with depth, the majority of water uptake during the dry part of the growing season occurred, on average, at less than similar to 60 cm soil depth throughout the catchment. While there were some trends in depth of water uptake related to genus, tree size and soil depth, water uptake was more uniformly shallow than we expected. Our results suggest that these types of forests may rely considerably on water sources that are quite shallow, even in the drier parts of the growing season. C1 [Gaines, Katie P.; Chen, Weile; Adams, Thomas S.; Lin, Henry; Eissenstat, David M.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Stanley, Jane W.] Penn State Univ, Dept Hort, University Pk, PA 16802 USA. [Meinzer, Frederick C.; Woodruff, David R.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97208 USA. [McCulloh, Katherine A.] Univ Wisconsin, Dept Bot, Madison, WI 53706 USA. RP Eissenstat, DM (reprint author), Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. EM dme9@psu.edu RI Lin, Henry/E-8234-2011 FU National Science Foundation [EAR 07-25019, EAR 12-39285, EAR 13-31726, 09-47962]; National Science Foundation FX Financial and logistical support and data were provided by National Science Foundation Grant EAR 07-25019 (C. Duffy), and EAR 12-39285 and EAR 13-31726 (S. Brantley) for the Susquehanna Shale Hills Critical Zone Observatory. Financial support during the creation of this manuscript was provided by National Science Foundation Grant CarbonEARTH (09-47962) to K.P.G. Funding to pay the Open Access publication charges for this article was provided by The National Science Foundation. NR 85 TC 2 Z9 2 U1 13 U2 25 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD APR PY 2016 VL 36 IS 4 BP 444 EP 458 DI 10.1093/treephys/tpv113 PG 15 WC Forestry SC Forestry GA DN9MP UT WOS:000377404000005 PM 26546366 ER PT J AU Williams, MR King, KW Ford, W Buda, AR Kennedy, CD AF Williams, Mark R. King, Kevin W. Ford, William Buda, Anthony R. Kennedy, Casey D. TI Effect of tillage on macropore flow and phosphorus transport to tile drains SO WATER RESOURCES RESEARCH LA English DT Article ID PREFERENTIAL FLOW; CLAY SOIL; HYDROGRAPH SEPARATION; HYDRAULIC-PROPERTIES; GRASSLAND SOILS; WATER; RUNOFF; PRECIPITATION; MANAGEMENT; RAINFALL AB Elevated phosphorus (P) concentrations in subsurface drainage water are thought to be the result of P bypassing the soil matrix via macropore flow. The objectives of this study were to quantify event water delivery to tile drains via macropore flow paths during storm events and to determine the effect of tillage practices on event water and P delivery to tiles. Tile discharge, total dissolved P (DP) and total P (TP) concentrations, and stable oxygen and deuterium isotopic signatures were measured from two adjacent tile-drained fields in Ohio, USA during seven spring storms. Fertilizer was surface-applied to both fields and disk tillage was used to incorporate the fertilizer on one field while the other remained in no-till. Median DP concentration in tile discharge prior to fertilizer application was 0.08 mg L-1 in both fields. Following fertilizer application, median DP concentration was significantly greater in the no-tilled field (1.19 mg L-1) compared to the tilled field (0.66 mg L-1), with concentrations remaining significantly greater in the no-till field for the remainder of the monitored storms. Both DP and TP concentrations in the no-till field were significantly related to event water contributions to tile discharge, while only TP concentration was significantly related to event water in the tilled field. Event water accounted for between 26 and 69% of total tile discharge from both fields, but tillage substantially reduced maximum contributions of event water. Collectively, these results suggest that incorporating surface-applied fertilizers has the potential to substantially reduce the risk of P transport from tile-drained fields. C1 [Williams, Mark R.; King, Kevin W.] USDA ARS, Soil Drainage Res Unit, Columbus, OH USA. [Ford, William] Marshall Univ, Huntington, WV USA. [Buda, Anthony R.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA USA. [Kennedy, Casey D.] USDA ARS, Pasture Syst & Watershed Management Res Unit, East Wareham, MA USA. RP Williams, MR (reprint author), USDA ARS, Soil Drainage Res Unit, Columbus, OH USA. EM mark.williams2@ars.usda.gov FU Nature Conservancy [48340001] FX The authors would like to thank the land owner of the study site who provided access to the fields; Jedediah Stinner, Katie Rumora, and Marie Schrecengost for help in data collection and site maintenance; and Sarah Fishel for analytical expertise. The authors would like to acknowledge the continued support of The Ohio State University in hosting the USDA-ARS Soil Drainage Research Unit and providing academic access to faculty resources, libraries, and students. The authors would also like to thank The Nature Conservancy for partial project funding (grant 48340001). This work was completed by the USDA-ARS and all data are in the public domain. For access to this data, contact Mark Williams. NR 46 TC 4 Z9 4 U1 15 U2 28 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD APR PY 2016 VL 52 IS 4 BP 2868 EP 2882 DI 10.1002/2015WR017650 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA DN9XA UT WOS:000377432800029 ER PT J AU Cohnstaedt, LW Ladner, J Campbell, LR Busch, N Barrera, R AF Cohnstaedt, Lee W. Ladner, Jamie Campbell, Leslie Rickert Busch, Noah Barrera, Roberto TI Determining Mosquito Distribution from Egg Data: The Role of the Citizen Scientist SO AMERICAN BIOLOGY TEACHER LA English DT Article DE Aedes aegypti; Aedes albopictus; mosquito surveillance; citizen science; epidemiology; invasive species; public health ID UNITED-STATES; AEGYPTI AB Nationwide science classes, from elementary through secondary, are placing a larger emphasis on inquiry and authentic experiences. The opportunity for community members (students, teachers, or interested individuals) to collect real data and contribute to a research project is the definition of citizen science. Recent disease outbreaks of mosquito-transmitted pathogens (West Nile, eastern equine encephalitis, dengue, chikungunya, and Zika) demonstrate the need to educate children and adults about the public health risks posed by mosquitoes. This lesson plan has students determine mosquito species and subsequent disease risk around their house and in their community by collecting mosquito eggs and rearing a portion of them to adults. The students identify adult mosquitoes and associated health risks. Furthermore, students and teachers have the option to participate in a national mosquito-species-distribution study by submitting mosquito eggs and adults to the U.S. Department of Agriculture. The data generated by participant submissions will be available to all mosquito submitters, making each student and school part of a larger project. This lesson plan has three objectives beyond the citizen science experience: (1) clarify the individual's role in protecting individuals, communities, and pets from illness; (2) raise awareness of pathogens transmitted by mosquitoes; and (3) participate in a national program to gather mosquito distribution data. The lesson and the associated supplementary material (available at http://www.citizenscience.us) can be used for middle to high school classes, as well as Advanced Placement classes, because the materials and presentations can be easily modified to classroom needs. C1 [Cohnstaedt, Lee W.] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. [Campbell, Leslie Rickert; Busch, Noah] Manhattan High Sch, 2100 Poyntz Ave, Manhattan, KS 66502 USA. [Barrera, Roberto] Ctr Dis Control & Prevent CDC, Entomol & Ecol Act, Dengue Branch, San Juan, PR 00920 USA. [Ladner, Jamie] Kansas State Univ, Manhattan, KS 66502 USA. RP Cohnstaedt, LW (reprint author), ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA.; Campbell, LR; Busch, N (reprint author), Manhattan High Sch, 2100 Poyntz Ave, Manhattan, KS 66502 USA.; Barrera, R (reprint author), Ctr Dis Control & Prevent CDC, Entomol & Ecol Act, Dengue Branch, San Juan, PR 00920 USA.; Ladner, J (reprint author), Kansas State Univ, Manhattan, KS 66502 USA. EM lee.cohnstaedt@ars.usda.gov; jlad011@ksu.edu; leslieca@usd383.org; noahb@usd383.org; rbarrera@cdc.gov NR 7 TC 0 Z9 0 U1 13 U2 15 PU NATL ASSOC BIOLOGY TEACHERS INC PI RESTON PA 12030 SUNRISE VALLEY DR, #110, RESTON, VA 20191 USA SN 0002-7685 EI 1938-4211 J9 AM BIOL TEACH JI Am. Biol. Teach. PD APR PY 2016 VL 78 IS 4 BP 317 EP 322 DI 10.1525/abt.2016.78.4.317 PG 6 WC Biology; Education, Scientific Disciplines SC Life Sciences & Biomedicine - Other Topics; Education & Educational Research GA DN0PT UT WOS:000376766500007 ER PT J AU O'Connor, A Anthony, R Bergamasco, L Coetzee, JF Dzikamunhenga, RS Johnson, AK Karriker, LA Marchant-Forde, JN Martineau, GP Millman, ST Pajor, EA Rutherford, K Sprague, M Sutherland, MA von Borell, E Webb, SR AF O'Connor, A. Anthony, R. Bergamasco, L. Coetzee, J. F. Dzikamunhenga, R. S. Johnson, A. K. Karriker, L. A. Marchant-Forde, J. N. Martineau, G. P. Millman, S. T. Pajor, E. A. Rutherford, K. Sprague, M. Sutherland, M. A. von Borell, E. Webb, S. R. TI Review: Assessment of completeness of reporting in intervention studies using livestock: an example from pain mitigation interventions in neonatal piglets SO ANIMAL LA English DT Review DE animal welfare; data collection; piglets; pain; reviews ID INFECTIOUS BOVINE KERATOCONJUNCTIVITIS; RANDOMIZED CONTROLLED-TRIALS; LOCAL-ANESTHESIA; INCREASING VALUE; REDUCING WASTE; ANIMAL-WELFARE; DAIRY CALVES; SAMPLE-SIZE; FOOD SAFETY; CASTRATION AB Accurate and complete reporting of study methods, results and interpretation are essential components for any scientific process, allowing end-users to evaluate the internal and external validity of a study. When animals are used in research, excellence in reporting is expected as a matter of continued ethical acceptability of animal use in the sciences. Our primary objective was to assess completeness of reporting for a series of studies relevant to mitigation of pain in neonatal piglets undergoing routine management procedures. Our second objective was to illustrate how authors can report the items in the Reporting guidElines For randomized controLled trials for livEstoCk and food safety (REFLECT) statement using examples from the animal welfare science literature. A total of 52 studies from 40 articles were evaluated using a modified REFLECT statement. No single study reported all REFLECT checklist items. Seven studies reported specific objectives with testable hypotheses. Six studies identified primary or secondary outcomes. Randomization and blinding were considered to be partially reported in 21 and 18 studies, respectively. No studies reported the rationale for sample sizes. Several studies failed to report key design features such as units for measurement, means, standard deviations, standard errors for continuous outcomes or comparative characteristics for categorical outcomes expressed as either rates or proportions. In the discipline of animal welfare science, authors, reviewers and editors are encouraged to use available reporting guidelines to ensure that scientific methods and results are adequately described and free of misrepresentations and inaccuracies. Complete and accurate reporting increases the ability to apply the results of studies to the decision-making process and prevent wastage of financial and animal resources. C1 [O'Connor, A.; Coetzee, J. F.; Dzikamunhenga, R. S.; Millman, S. T.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Coll Vet Med, Ames, IA 50011 USA. [Anthony, R.] Univ Alaska Anchorage, Dept Philosophy, Anchorage, AK 99508 USA. [Bergamasco, L.] Virginia Tech, Dept Anim & Poultry Sci, Coll Agr & Life Sci, Blacksburg, VA 24060 USA. [Johnson, A. K.] Iowa State Univ, Dept Anim Sci, Coll Agr & Life Sci, Ames, IA 50011 USA. [Marchant-Forde, J. N.] Purdue Univ, USDA ARS, Livestock Behav Res Unit, W Lafayette, IN 47907 USA. [Martineau, G. P.] Swine Clin, Natl Vet Sch, Dept Anim Prod, F-31076 Toulouse, France. [Millman, S. T.] Iowa State Univ, Dept Biomed Sci, Coll Vet Med, Ames, IA 50011 USA. [Rutherford, K.] Scotlands Rural Coll SRUC, Edinburgh EH9 3JG, Midlothian, Scotland. [Sutherland, M. A.] AgResearch Ltd, Ruakura Res Ctr, Hamilton 3240, New Zealand. [Karriker, L. A.; Sprague, M.] AASV, Perry, IA 50220 USA. [von Borell, E.] Univ Halle Wittenberg, Inst Agr & Nutr Sci, Dept Anim Husb & Ecol, D-06108 Halle, Saale, Germany. [Webb, S. R.] Natl Pork Board, Des Moines, IA 50325 USA. [Pajor, E. A.] Univ Calgary, Fac Vet Med, Dept Prod Anim Hlth, Calgary, AB T2N 1N4, Canada. RP O'Connor, A (reprint author), Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Coll Vet Med, Ames, IA 50011 USA. EM oconnor@iastate.edu RI Rutherford, Kenneth/I-7638-2014; OI Rutherford, Kenneth/0000-0003-1944-3023; Marchant-Forde, Jeremy/0000-0002-5287-2914; Millman, Suzanne/0000-0001-5752-4647; O'Connor, A/0000-0003-0604-7822; Sutherland, Mhairi/0000-0001-6972-2405 FU National Pork Board (NPB) Grant [12-186] FX This project was funded by the National Pork Board (NPB) Grant no. 12-186. The authors have no conflicts of interest to declare. NR 34 TC 0 Z9 0 U1 4 U2 10 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD APR PY 2016 VL 10 IS 4 BP 660 EP 670 DI 10.1017/S1751731115002323 PG 11 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA DN5PY UT WOS:000377123800016 PM 26556522 ER PT J AU Sun, R Urban, JF Notari, L Vanuytsel, T Madden, KB Bohl, JA Ramalingam, TR Wynn, TA Zhao, AP Shea-Donohue, T AF Sun, Rex Urban, Joseph F., Jr. Notari, Luigi Vanuytsel, Tim Madden, Kathleen B. Bohl, Jennifer A. Ramalingam, Thirumalai R. Wynn, Thomas A. Zhao, Aiping Shea-Donohue, Terez TI Interleukin-13 Receptor alpha 1-Dependent Responses in the Intestine Are Critical to Parasite Clearance SO INFECTION AND IMMUNITY LA English DT Article ID ALTERNATIVELY ACTIVATED MACROPHAGES; EPITHELIAL-CELL FUNCTION; NIPPOSTRONGYLUS-BRASILIENSIS INFECTION; HELIGMOSOMOIDES-POLYGYRUS; PROTECTIVE IMMUNITY; NEMATODE INFECTIONS; ENTERIC NEMATODES; TH2 CYTOKINE; MURINE GUT; T-CELLS AB Nematode infection upregulates interleukin-4 (IL-4) and IL-13 and induces STAT6-dependent changes in gut function that promote worm clearance. IL-4 and IL-13 activate the type 2 IL-4 receptor (IL-4R), which contains the IL-13R alpha 1 and IL-4R alpha chains. We used mice deficient in IL-13R alpha 1 (IL-13R alpha 1(-/-)) to examine the contribution of IL-13 acting at the type 2 IL-4R to immune and functional responses to primary (Hb1) and secondary (Hb2) infections with the gastrointestinal nematode parasite Heligmosomoides bakeri. There were differences between strains in the IL-4 and IL-13 expression responses to Hb1 but not Hb2 infection. Following Hb2 infection, deficient mice had impaired worm expulsion and higher worm fecundity despite normal production of Th2-derived cytokines. The upregulation of IL-25 and IL-13R alpha 2 in Hb1- and Hb2-infected wild-type (WT) mice was absent in IL-13R alpha 1(-/-) mice. Goblet cell numbers and resistin-like molecule beta (RELM-beta) expression were attenuated significantly in IL-13R alpha 1(-/-) mice following Hb2 infections. IL-13R alpha 1 contributes to the development of alternatively activated macrophages, but the type 1 IL-4R is also important. Hb1 infection had no effects on smooth muscle function or epithelial permeability in either strain, while the enhanced mucosal permeability and changes in smooth muscle function and morphology observed in response to Hb2 infection in WT mice were absent in IL-13R alpha 1(-/-) mice. Notably, the contribution of claudin-2, which has been linked to IL-13, does not mediate the increased mucosal permeability following Hb2 infection. These results show that activation of IL-13R alpha 1 is critical for key aspects of the immune and functional responses to Hb2 infection that facilitate expulsion. C1 [Sun, Rex; Notari, Luigi; Bohl, Jennifer A.; Zhao, Aiping; Shea-Donohue, Terez] Univ Maryland, Sch Med, Dept Radiat Oncol, Baltimore, MD 21201 USA. [Sun, Rex; Notari, Luigi; Bohl, Jennifer A.; Zhao, Aiping; Shea-Donohue, Terez] Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. [Vanuytsel, Tim] Univ Leuven, Translat Res Ctr Gastrointestinal Disorders TARGI, Leuven, Belgium. [Madden, Kathleen B.] Uniformed Serv Univ Hlth Sci, Dept Pediat, Bethesda, MD 20814 USA. [Ramalingam, Thirumalai R.; Wynn, Thomas A.] NIAID, Div Parasitol, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. RP Shea-Donohue, T (reprint author), Univ Maryland, Sch Med, Dept Radiat Oncol, Baltimore, MD 21201 USA.; Shea-Donohue, T (reprint author), Univ Maryland, Sch Med, Dept Med, Baltimore, MD 21201 USA. EM tdonohue@mbrc.umaryland.edu FU NIH [R01-AI/DK49316, R01-DK083418]; USDA [1235-51000-055] FX NIH provided funding to Terez Shea-Donohue under grant number R01-AI/DK49316. NIH provided funding to Aiping Zhao under grant number R01-DK083418. USDA provided funding to Joseph F. Urban under grant number CRIS project #1235-51000-055. NR 41 TC 2 Z9 2 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD APR PY 2016 VL 84 IS 4 BP 1032 EP 1044 DI 10.1128/IAI.00990-15 PG 13 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA DN5JV UT WOS:000377103600015 PM 26810038 ER EF