FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Roberts-Clarke, D Fornusek, C Singh, MAF Burns, J Hackett, DA AF Roberts-Clarke, Daniel Fornusek, Che Singh, Maria A. Fiatarone Burns, Joshua Hackett, Daniel A. TI Examining hand dominance using dynamometric grip strength testing as evidence for overwork weakness in Charcot-Marie-Tooth disease: a systematic review and meta-analysis SO INTERNATIONAL JOURNAL OF REHABILITATION RESEARCH LA English DT Review DE Charcot-Marie-Tooth disease; exercise; Hereditary motor and sensory neuropathy; systematic review ID PHYSICAL-ACTIVITY; HYPOTHESIS; NEUROPATHY AB This systematic review with a meta-analysis of studies was carried out to evaluate the potential of overwork weakness on the basis of grip strength of dominant and nondominant hands in individuals with Charcot-Marie-Tooth disease (CMT). Numerous electronic databases were searched from the earliest records to February 2016. Studies of any design including participants older than 18 years of age with a confirmed diagnosis of CMT that measured grip strength of both hands using dynamometric testing were eligible for inclusion. Of 12 593 articles identified following removal of duplicates, five articles fulfilled the criteria. A total of 166 participants, mostly with CMT1 or CMT2, were described from the studies included. Hand and finger pinch grip strength for the dominant compared with the nondominant hand was not statistically different. There is no definitive evidence that preferential use of the dominant hand in CMT impairs function relative to the nondominant hand. Thus, robust exercise trials of progressive resistance training are needed to understand the extent of adaptations possible and provide evidence of the safety of such regimens. Copyright (C) 2016 Wolters Kluwer Health, Inc. All rights reserved. C1 [Roberts-Clarke, Daniel; Fornusek, Che; Singh, Maria A. Fiatarone; Hackett, Daniel A.] Univ Sydney, Exercise Hlth & Performance Fac Res Grp, Lidcombe, NSW, Australia. [Burns, Joshua] Univ Sydney, Arthrit & Musculoskeletal Res Grp, Discipline Physiotherapy, Fac Hlth Sci, Lidcombe, NSW, Australia. [Singh, Maria A. Fiatarone] Univ Sydney, Sydney Med Sch, Charles Perkins Ctr, Lidcombe, NSW, Australia. [Burns, Joshua] Childrens Hosp Westmead, Neurosci & Muscle Res Grp, Westmead, NSW, Australia. [Burns, Joshua] Sydney Childrens Hosp Network, Paediat Gait Anal Serv New South Wales, Randwick, NSW, Australia. [Burns, Joshua] Sydney Childrens Hosp Network, Paediat Gait Anal Serv New South Wales, Westmead, NSW, Australia. [Singh, Maria A. Fiatarone] Harvard Med Sch, Hebrew SeniorLife, Boston, MA USA. [Singh, Maria A. Fiatarone] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Hackett, DA (reprint author), Univ Sydney, Fac Hlth Sci, Exercise Hlth & Performance Fac Res Grp, 75 East St, Lidcombe, NSW 2141, Australia. EM daniel.hackett@sydney.edu.au NR 35 TC 0 Z9 0 U1 5 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0342-5282 EI 1473-5660 J9 INT J REHABIL RES JI Int. J. Rehabil. Res. PD SEP PY 2016 VL 39 IS 3 BP 189 EP 196 DI 10.1097/MRR.0000000000000174 PG 8 WC Rehabilitation SC Rehabilitation GA DS4PP UT WOS:000380763300001 PM 27177353 ER PT J AU Wang, Y Shao, L Wang, J Ren, H Liu, H Zhang, QM Guo, QF Chen, XW AF Wang, Y. Shao, L. Wang, J. Ren, H. Liu, H. Zhang, Q. M. Guo, Q. F. Chen, X. W. TI Comparison of morphological and physiological characteristics in two phenotypes of a rare and endangered plant, Begonia fimbristipula Hance SO PHOTOSYNTHETICA LA English DT Article DE anatomy; epigenetic; macronutrient; morphology; pigment ID MULTI PETAL JASMINE; CHLOROPHYLL FLUORESCENCE; CHLOROPLAST ULTRASTRUCTURE; DIFFERENTIAL RESPONSES; EPIGENETIC INHERITANCE; NUTRIENT LIMITATION; CHANGING CLIMATE; LIFE-HISTORY; PLASTICITY; LEAVES AB The rare and endangered plant, Begonia fimbristipula, shows red and green phenotypes, differentiated by a coloration of the abaxial leaf surface. In this study, we compared morphological and physiological traits of both phenotypes. The results showed that the red phenotype contained a significantly higher chlorophyll content, closer arrangement of chloroplasts, and a more developed grana. In addition, the red phenotype transferred significantly more light energy into the electron transport during the photoreaction. Similarly, the maximum photosynthetic rate, instantaneous water-use and light-use efficiencies of the red B. fimbristipula were all significantly higher than those of the green individuals. The differentiation between these two phenotypes could be caused by their different survival strategies under the same conditions; epigenetic variations may be in some correlation with this kind of phenotype plasticity. Red B. fimbristipula has an advantage in resource acquisition and utilization and possesses a better self-protection mechanism against changes in environmental conditions, therefore, it might adapt better to global climate change compared to the green phenotype. Further studies on the possible epigenetic regulation of those phenotypic differentiations are needed. C1 [Wang, Y.; Wang, J.; Ren, H.; Zhang, Q. M.] Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China. [Shao, L.; Chen, X. W.] Zhaoqing Univ, Coll Life Sci, Zhaoqing 526061, Peoples R China. [Liu, H.] Florida Int Univ, Dept Earth & Environm, Int Ctr Trop Bot, Miami, FL 33199 USA. [Liu, H.] Guangxi Univ, Coll Forestry, Nanning 530004, Peoples R China. [Guo, Q. F.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Res Triangle Pk, NC 27709 USA. RP Ren, H (reprint author), Chinese Acad Sci, Key Lab Vegetat Restorat & Management Degraded Ec, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China. EM renhai@scbg.ac.cn FU Guangzhou Science and Technology Program [2014J4500035]; National Natural Science Foundation of China [31570422] FX The authors express their gratitude to Dr. Nan Liu and Mr. Zhifang Lin for valuable suggestions for this research; to Dr. Shuguang Jian and Shizhong Liu for fieldwork assistance; to Mrs. Chunqing Long, Mrs. Rufang Deng, and Mrs. Xiaoying Hu for index measurements. This research was supported by Guangzhou Science and Technology Program (2014J4500035), and National Natural Science Foundation of China (31570422). Thanks are also due to Prof. Jennifer Richards and Ms. Elizabeth Hamblin for English editing and constructive comments. NR 67 TC 0 Z9 0 U1 20 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0300-3604 EI 1573-9058 J9 PHOTOSYNTHETICA JI Photosynthetica PD SEP PY 2016 VL 54 IS 3 BP 381 EP 389 DI 10.1007/s11099-016-0199-5 PG 9 WC Plant Sciences SC Plant Sciences GA DS5BS UT WOS:000380796100008 ER PT J AU Ali, SS Amoako-Attah, I Bailey, RA Strem, MD Schmidt, M Akrofi, AY Surujdeo-Maharaj, S Kolawole, OO Begoude, BAD ten Hoopen, GM Goss, E Phillips-Mora, W Meinhardt, LW Bailey, BA AF Ali, S. S. Amoako-Attah, I. Bailey, R. A. Strem, M. D. Schmidt, M. Akrofi, A. Y. Surujdeo-Maharaj, S. Kolawole, O. O. Begoude, B. A. D. ten Hoopen, G. M. Goss, E. Phillips-Mora, W. Meinhardt, L. W. Bailey, B. A. TI PCR-based identification of cacao black pod causal agents and identification of biological factors possibly contributing to Phytophthora megakarya's field dominance in West Africa SO PLANT PATHOLOGY LA English DT Article DE appressorial; cocoa; Ghana; PCR; phylogeny; virulence ID THEOBROMA-CACAO; RESISTANCE; COCOA; PHYLOGENY; PALMIVORA; MARKERS; SEQUENCES; YIELD; DNA AB Among the Phytophthora species that cause black pod of cacao, P. megakarya is the most virulent, posing a serious threat to cacao production in Africa. Correct identification of the species causing the black pod and understanding the virulence factors involved are important for developing sustainable disease management strategies. A simple PCR-based species identification method was developed using the species-specific sequences in the ITS regions of the rRNA gene. A phylogenetic tree generated for 119 Phytophthora isolates, based on the 60S ribosomal protein L10 gene and rDNA sequence, verified the PCR-based identification assay and showed high interspecific variation among the species causing black pod. Phytophthora megakarya isolates were uniformly virulent in an assay using susceptible cacao pod husks inoculated with zoospores, while the P. palmivora isolates showed greater divergence in virulence. The virulence of P. megakarya was associated with earlier production of sporangia and an accelerated induction of necrosis. While zoospore germ tubes of both species penetrated pods through stomata, only P. megakarya produced significant numbers of appressoria. A hypersensitive-like response was observed when attached SCA-6 pods were inoculated with P. palmivora. SCA-6 pods became vulnerable to P. palmivora when wounded prior to zoospore inoculation. Phytophthora megakarya was more aggressive than P. palmivora on attached SCA-6 pods, causing expanding necrotic lesions with or without wounding. Phytophthora megakarya is predominant in the Volta region of Ghana and it remains to be seen whether it can displace P. palmivora from cacao plantations of Ghana as it has in Nigeria and Cameroon. C1 [Ali, S. S.; Amoako-Attah, I.; Bailey, R. A.; Strem, M. D.; Schmidt, M.; Meinhardt, L. W.; Bailey, B. A.] USDA ARS, Sustainable Perennial Crops Lab, Inst Plant Sci, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Amoako-Attah, I.; Akrofi, A. Y.] Cocoa Res Inst, Akim New Tafo, Ghana. [Surujdeo-Maharaj, S.] Univ West Indies, Cocoa Res Ctr, St Augustine, Trinid & Tobago. [Kolawole, O. O.] Cocoa Res Inst Nigeria, Ibadan, Nigeria. [Kolawole, O. O.; Goss, E.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Begoude, B. A. D.; ten Hoopen, G. M.] IRAD, Reg Lab Biol & Appl Microbiol, BP 2067, Yaounde, Cameroon. [ten Hoopen, G. M.] CIRAD, UPR Bioagresseurs 106, F-34398 Montpellier, France. [Phillips-Mora, W.] CATIE, Dept Agr & Agroforestry, Turrialba 7170, Costa Rica. RP Bailey, BA (reprint author), USDA ARS, Sustainable Perennial Crops Lab, Inst Plant Sci, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. EM bryan.bailey@ars.usda.gov FU USDA ARS; Global Cocoa Initiative FX This work was funded by USDA ARS. References to a company and/or product by the USDA are only for the purposes of information and do not imply approval or recommendation of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. The authors thank Drs Cristiano Villela Dias and Jean-Philippe Marelli of Mars Center for Cocoa Science-Cp 55 Itajuipe Ba, Brazil for providing DNA samples of Phytophthora isolates. I.A.-A. thanks Borlaug Global Cocoa Initiative for travel grants to visit the USDA-ARS laboratory. The authors have no conflict of interest to declare. NR 37 TC 1 Z9 1 U1 2 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD SEP PY 2016 VL 65 IS 7 BP 1095 EP 1108 DI 10.1111/ppa.12496 PG 14 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DS6IU UT WOS:000380886300005 ER PT J AU Kuhnem, PR Ward, TJ Silva, CN Spolti, P Ciliato, ML Tessmann, DJ Del Ponte, EM AF Kuhnem, P. R. Ward, T. J. Silva, C. N. Spolti, P. Ciliato, M. L. Tessmann, D. J. Del Ponte, E. M. TI Composition and toxigenic potential of the Fusarium graminearum species complex from maize ears, stalks and stubble in Brazil SO PLANT PATHOLOGY LA English DT Article DE Fusarium meridionale; gibberella ear rot; nivalenol; Zea mays ID HEAD BLIGHT; POPULATION-STRUCTURE; GENEALOGICAL CONCORDANCE; MYCOTOXIN PRODUCTION; SOUTHERN BRAZIL; EASTERN CHINA; WHEAT; BARLEY; MERIDIONALE; RESISTANCE AB A large collection (n=539) of Fusarium graminearum species complex (FGSC) isolates was obtained from Brazilian maize, and collections formed according to geography and maize part: (i) kernel (n=110) from south and south-central Brazil; (ii) stalk (n=134) from Parana state (south); and (iii) stubble (n=295) from Rio Grande do Sul state (south). Species composition, identified using a multilocus genotype approach, was assessed separately in each collection due to differences in geographic sampling. Overall, three species were found: F.meridionale (Fmer; 67% prevalence) with the nivalenol (NIV) genotype, F.graminearum (Fgra; 19%) with the 15-acetyl (A) deoxynivalenol (DON) genotype, and F.cortaderiae (Fcor; 14%) with the NIV (49/74) or the 3-ADON (25/74) genotype. In kernels, Fmer was spread across all locations and Fgra and Fcor were found mostly at high elevation (>800m a.s.l.). The majority (978%) of stalk isolates was assigned to Fmer; three were assigned to Fgra. In the stubble, Fmer was less dominant (53%), with a shift towards Fcor as the most frequent species at high elevation sites (>600m a.s.l.). No differences in the mycelial growth rate were observed among isolates from each species grown at 15 degrees C. Fgra grew faster at 25 degrees C and Fmer showed the widest range of variation across the isolates at both temperatures. The survey data suggest that Fmer may outcompete other species on ears and stalks in comparison to stubble. Additional sampling that controls for other factors, as well as direct testing of aggressiveness on ears and stalk tissue, will be needed to fully evaluate this hypothesis. C1 [Kuhnem, P. R.; Spolti, P.; Del Ponte, E. M.] Univ Fed Rio Grande do Sul, Dept Fitossanidade, BR-91540000 Porto Alegre, RS, Brazil. [Ward, T. J.] USDA ARS, Peoria, IL USA. [Silva, C. N.; Ciliato, M. L.; Tessmann, D. J.] Univ Estadual Maringa, Dept Agron, Maringa, Parana, Brazil. [Del Ponte, E. M.] Univ Fed Vicosa, Dept Fitopatol, BR-36570900 Vicosa, MG, Brazil. RP Del Ponte, EM (reprint author), Univ Fed Rio Grande do Sul, Dept Fitossanidade, BR-91540000 Porto Alegre, RS, Brazil.; Del Ponte, EM (reprint author), Univ Fed Vicosa, Dept Fitopatol, BR-36570900 Vicosa, MG, Brazil. EM delponte@ufv.br OI Del Ponte, Emerson/0000-0003-4398-409X FU Programa de Pos-graduacao em Fitotecnia (UFRGS); Conselho Nacional de desenvolvimento Cientifico e Tecnologico - CNPq; CNPq; USDA-ARS National Program for Food Safety FX Thanks are given to the Programa de Pos-graduacao em Fitotecnia (UFRGS) and Conselho Nacional de desenvolvimento Cientifico e Tecnologico - CNPq for providing a graduate scholarship to P.R.K. and to CNPq for providing a research fellowship to E.M.D.P. and D.J.T. This study was partially supported by the USDA-ARS National Program for Food Safety. The authors are indebted to Nathane Orwig and Thomas Usgaard for excellent technical support. NR 36 TC 2 Z9 2 U1 5 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD SEP PY 2016 VL 65 IS 7 BP 1185 EP 1191 DI 10.1111/ppa.12497 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DS6IU UT WOS:000380886300014 ER PT J AU Moon, J Ma, L Xia, K Williams, MA AF Moon, Jinyoung Ma, Li Xia, Kang Williams, Mark A. TI Plant - Microbial and mineral contributions to amino acid and protein organic matter accumulation during 4000 years of pedogenesis SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Lake Michigan chronosequence; Sand dune; Pedogenesis; Soil organic matter (SOM); Soil organic nitrogen (SON); Soil protein; Hydrolysable amino acids; Organo-mineral associations ID MICHIGAN SAND DUNES; SELECTIVE PRESERVATION; LITTER DECOMPOSITION; NITROGEN STORAGE; CELL-WALL; SOIL; STABILIZATION; CARBON; BIODEGRADATION; SORPTION AB The dynamics and persistence of proteinaceous compounds during pedogenesis are major mechanisms of soil formation and determinants of organic matter (OM) turnover. We investigated the accumulation patterns of proteinogenic amino acids associated with minerals dominated by permanently negative charges (primary silica minerals) and related these to vegetative and belowground microbial succession during soil ecosystem development. Positively-charged amino acids (arginine, lysine, histidine), extracted from whole soil pool using 6 M HCl, showed clear patterns of accumulation, increasing similar to 65% during 4010 years of development, while negatively charged amino acids (glutamic acid, aspartic acid) decreased similar to 13%. In the mineral associated sub-pool, positively charged amino acids were approximately similar to 431% more enriched, while negatively charged amino acids were similar to 38% depleted as compared to the whole soil pool. The multivariate ordination of soil bacterial community structure based on a 16s ribosomal RNA gene analysis and that of the aboveground plant community structure predicted 71% (p < 0.0001) and 66% (p < 0.0001) of the amino acid dynamics, respectively, during soil ecosystem development. Ala-rich Actinobacteria abundance declined with the year of development, concomitant with the decrease of Ala content in soil (r(2) = 0.82, p = 0.0019). His-rich Acidobacteria and His in soil both increased with the year of development (r(2) = 0.92, p = 0.0022). In support of the main hypothesis, the relative distribution of proteinogenic amino acids changed during pedogenesis with evidence indicating that biological communities and minerals play roles as source and sink of OM in soil, respectively. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Moon, Jinyoung; Williams, Mark A.] Virginia Polytech Inst & State Univ, Dept Hort, Soil Microbial Ecol & Biogeochem Lab, 312 Latham Hall,220 Ag Quad Ln, Blacksburg, VA 24061 USA. [Ma, Li] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Ma, Li] USDA ARS, Soil Phys & Pesticides Res Unit, George E Brown Jr Salin Lab, Riverside, CA 92507 USA. [Ma, Li; Xia, Kang] Virginia Polytech Inst & State Univ, Dept Crop & Soil Environm Sci, 1880 Pratt Dr, Blacksburg, VA 24061 USA. RP Williams, MA (reprint author), Virginia Polytech Inst & State Univ, Dept Hort, Soil Microbial Ecol & Biogeochem Lab, 312 Latham Hall,220 Ag Quad Ln, Blacksburg, VA 24061 USA. EM markwill@vt.edu FU United States Department of Agriculture National Institute of Food and Agriculture Foundational Programs [2011-03815] FX This research was funded by the United States Department of Agriculture National Institute of Food and Agriculture Foundational Programs (grant# 2011-03815). We thank Drs. Brian D. Strahm, Richard F. Helm, Richard E. Veilleux, Richard Rodrigues, Ms. Kerri Mills, and Hua Xiao for insightful suggestions and comments on this work. We acknowledge Dr. Shankar G. Shanmugam for collecting soil samples from Lake Michigan chronosequence, Dr. Madhavi L. Kakumanu for the density fractionation of soils, and Dr. Chao Shang for technical advice on the HPLC instrumentation. We also thank the technical staffs at the National Synchrotron Light Sources, Brookhaven National Laboratory. NR 57 TC 0 Z9 0 U1 19 U2 20 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD SEP PY 2016 VL 100 BP 42 EP 50 DI 10.1016/j.soilbio.2016.05.011 PG 9 WC Soil Science SC Agriculture GA DS2LD UT WOS:000380600100005 ER PT J AU Chang, CH Szlavecz, K Buyer, JS AF Chang, Chih-Han Szlavecz, Katalin Buyer, Jeffrey S. TI Species-specific effects of earthworms on microbial communities and the fate of litter-derived carbon SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Earthworm; Phospholipid fatty acid analysis; Soil respiration; Stable isotope; Structural equation modeling ID SOIL ORGANIC-MATTER; NORTHERN TEMPERATE FORESTS; INVASIVE EARTHWORMS; HARDWOOD FOREST; EXOTIC EARTHWORMS; AMYNTHAS-AGRESTIS; LAND-USE; DENDROBAENA OCTAEDRA; DECIDUOUS FORESTS; PINE FOREST AB Soil respiration is frequently measured as a surrogate for biological activities and is important in soil carbon cycling. The heterotrophic component of soil respiration is primarily driven by microbial decomposition of leaf litter and soil organic matter, and is partially controlled by resource availability. In North American temperate deciduous forests, invasive European and Asian earthworms are known to variously affect soil properties and resource availability through their feeding, burrowing, and casting behaviors, and may affect different components of soil respiration through modulating the microbial communities. By tracing litter-derived C from C-13 and N-15 double-enriched leaf litter into soil and CO2 efflux in a mesocosm experiment, we tested the hypothesis that earthworms inhibit litter C-derived soil respiration by reducing resource availability and microbial biomass, and further examined how species specific effects of earthworms on soil respiration are mediated by soil microbial community. We showed that while earthworms generally had no effect on total soil respiration, the interaction between Octolasion lacteum and Lumbricus rubellus had a significant negative non-additive effect, presumably through affecting anaerobic microsites in the soil. Moreover, litter C-derived soil respiration was reduced by the Asian Amynthas hilgendorfi, the European L. rubellus, and the North American native species Eisenoides lonnbergi, but not by the European species O. lacteum. Phospholipid fatty acid (PLFA) analysis and structural equation modeling indicated that while soil bacteria and fungi abundances were affected by earthworm species identities, the observed reduction of litter C-derived soil respiration could not be fully explained by changes in microbial biomass. We attributed these effects to earthworm-induced aggregate formation, reduction of microbial transformation of labile carbon, and antimicrobial peptide activities, and concluded that the mechanisms through which the four earthworm species affect the fate of litter derived C and its mineralization are species-specific. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Chang, Chih-Han; Szlavecz, Katalin] Johns Hopkins Univ, Dept Earth & Planetary Sci, 3400 North Charles St, Baltimore, MD 21210 USA. [Buyer, Jeffrey S.] ARS, USDA, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. RP Chang, CH (reprint author), Johns Hopkins Univ, Dept Earth & Planetary Sci, 3400 North Charles St, Baltimore, MD 21210 USA. EM cchang9@jhu.edu FU EPS Field Funds; National Science Foundation [EEC-0540832, ACI 1244820, EAR-0748574]; Microsoft Research FX We are grateful to the people who helped in the field and during the lab experiments, especially Kelly Baker, Zachary Ferguson, Adam Dec and Jia-Hsing Wu. We also thank Stanley Tesch for helping with PLFA, Chuan-Chin Huang for insights on data analysis, and Jim Grace for suggestions on SEM. We appreciate two anonymous reviewers for providing positive, insightful, and helpful comments on an earlier version of this manuscript. Funding for this study was partially provided by the EPS Field Funds, the National Science Foundation (EEC-0540832, ACI 1244820, and EAR-0748574), and Microsoft Research. NR 77 TC 1 Z9 1 U1 46 U2 63 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD SEP PY 2016 VL 100 BP 129 EP 139 DI 10.1016/j.soilbio.2016.06.004 PG 11 WC Soil Science SC Agriculture GA DS2LD UT WOS:000380600100016 ER PT J AU Cummings, KJ Rodriguez-Rivera, LD Grigar, MK Rankin, SC Mesenbrink, BT Leland, BR Bodenchuk, MJ AF Cummings, K. J. Rodriguez-Rivera, L. D. Grigar, M. K. Rankin, S. C. Mesenbrink, B. T. Leland, B. R. Bodenchuk, M. J. TI Prevalence and Characterization of Salmonella Isolated from Feral Pigs Throughout Texas SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Epidemiology; foodborne disease; public health; Salmonella spp; swine; zoonoses ID ESCHERICHIA-COLI O157-H7; BOARS SUS-SCROFA; UNITED-STATES; WILD BOARS; ANTIMICROBIAL SUSCEPTIBILITY; DISEASE TRANSMISSION; SWINE; CATTLE; PATHOGENS; SPP. AB Feral pigs are one of the most abundant free-roaming ungulates in the United States, yet their role in the ecology and transmission of foodborne pathogens is poorly understood. Our objectives were to estimate the prevalence of Salmonella shedding among feral pigs throughout Texas, to identify risk factors for infection, and to characterize the isolates. Faecal samples were collected from feral pigs in Texas from June 2013 through May 2015. Standard bacteriologic culture methods were used to isolate Salmonella from samples, and isolates were characterized via serotyping and anti-microbial susceptibility testing. The prevalence of faecal Salmonella shedding among sampled pigs was 43.9% (194/442), with positive pigs originating from 50 counties. Pigs sampled during fall and summer were significantly more likely to be shedding Salmonella than pigs sampled during winter. High serovar diversity was evident among the isolates, and many of the detected serovars are leading causes of human salmonellosis. The most common serovars were Montevideo (10.0%), Newport (9.1%), and Give (8.2%). Resistance to anti-microbial agents was rare. The burgeoning feral pig population in the United States may represent an emerging threat to food safety. C1 [Cummings, K. J.; Rodriguez-Rivera, L. D.; Grigar, M. K.] Texas A&M Univ, College Stn, TX USA. [Rankin, S. C.] Univ Penn, Philadelphia, PA 19104 USA. [Mesenbrink, B. T.; Leland, B. R.; Bodenchuk, M. J.] USDA, San Antonio, TX USA. RP Cummings, KJ (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Integrat Biosci, 4458 TAMU, College Stn, TX 77843 USA. EM kcummings@cvm.tamu.edu FU USDA National Institute of Food and Agriculture Formula Animal Health Fund FX We thank all participating wildlife biologists who submitted samples over the duration of the study. This research was supported in part by the USDA National Institute of Food and Agriculture Formula Animal Health Fund. NR 37 TC 1 Z9 1 U1 25 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD SEP PY 2016 VL 63 IS 6 BP 436 EP 441 DI 10.1111/zph.12244 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA DS7IY UT WOS:000380958100002 PM 26614102 ER PT J AU McRoberts, RE Westfall, JA AF McRoberts, Ronald E. Westfall, James A. TI Propagating uncertainty through individual tree volume model predictions to large-area volume estimates SO ANNALS OF FOREST SCIENCE LA English DT Article DE Measurement error; Parameter uncertainty; Residual variability; Stratified estimation ID NATIONAL FOREST INVENTORY; GROWING STOCK VOLUME; MEASUREMENT ERRORS; TROPICAL FORESTS; VARIABILITY AB The effects on large-area volume estimates of uncertainty in individual tree volume model predictions were negligible when using simple random sampling estimators for large-area estimation, but non-negligible when using stratified estimators which reduced the effects of sampling variability. Forest inventory estimates of tree volume for large areas are typically calculated by adding model predictions of volumes for individual trees at the plot level and calculating the per unit area mean over plots. The uncertainty in the model predictions is generally ignored with the result that the precision of the large-area volume estimate is optimistic. The primary objective was to estimate the effects on large-area volume estimates of volume model prediction uncertainty due to diameter and height measurement error, parameter uncertainty, and model residual variance. Monte Carlo simulation approaches were used because of the complexities associated with multiple sources of uncertainty, the non-linear nature of the models, and heteroskedasticity. The effects of model prediction uncertainty on large-area volume estimates of growing stock volume were negligible when using simple random sampling estimators. However, with stratified estimators that reduce the effects of sampling variability, the effects of model prediction uncertainty were not necessarily negligible. The adverse effects of parameter uncertainty and residual variance were greater than the effects of diameter and height measurement errors. The uncertainty of large-area volume estimates that do not account for model prediction uncertainty should be regarded with caution. C1 [McRoberts, Ronald E.] US Forest Serv, Northern Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. [Westfall, James A.] US Forest Serv, Northern Res Stn, Newtown Sq, PA USA. RP McRoberts, RE (reprint author), US Forest Serv, Northern Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us; jameswestfall@fs.fed.us NR 31 TC 2 Z9 3 U1 8 U2 8 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1286-4560 EI 1297-966X J9 ANN FOREST SCI JI Ann. For. Sci. PD SEP PY 2016 VL 73 IS 3 BP 625 EP 633 DI 10.1007/s13595-015-0473-x PG 9 WC Forestry SC Forestry GA DR9ZA UT WOS:000380253600006 ER PT J AU Klauberg, C Vidal, E Silva, CA Bentes, MD Hudak, AT AF Klauberg, Carine Vidal, Edson Silva, Carlos Alberto Bentes, Michelliny de M. Hudak, Andrew Thomas TI Sampling methods for titica vine (Heteropsis spp.) inventory in a tropical forest SO ANNALS OF FOREST SCIENCE LA English DT Article DE Heteropsis; Inventory; Non-timber forest product; Simulation; Araceae; Hemiepiphyte; Random plot; Sampling scheme; Systematic plot; Tropical forest ID ARACEAE; AMAZON; PRODUCTS; BRAZIL AB Titica vine provides useful raw fiber material. Using sampling schemes that reduce sampling error can provide direction for sustainable forest management of this vine. Sampling systematically with rectangular plots (10 x 25 m) promoted lower error and greater accuracy in the inventory of titica vines in tropical rainforest The titica vine (Heteropsis spp.) is an important raw material for native communities located in the Amazon tropical rainforest. However, variability and spatial distribution of this species are some factors that hinder the assessment of accurate productivity prediction, and thus, inventory methods for management plan must be defined. The aim of this study was to develop and compare accuracy of different sampling approaches for the inventory of titica vines (roots ha(-1)) using simulations based on field data. Eighty-eight treatments were defined by the combination of the process (random or systematic), sampling intensity (5, 10, 15, and 20 % of 18 ha), plot size (250 to 3000 m(2)) and shapes (square, rectangle, and transect). Production estimate, sampling error, and relative efficiency were used to compare treatments with each other and with the reference inventory. The mean production estimates for the random and systematic processes were 570 and 597 vine roots ha(-1), while the reference estimate was 500 vine roots ha(-1). Among of the treatments evaluated, only 5.8 % of them provided a sampling error less than 15 %. The sampling procedure that combines systematic or random process, plots sizes of 250 or 300 m(2) and sampling intensity greater than 15 % was the most efficient for inventory of titica vine in tropical forests. C1 [Klauberg, Carine; Vidal, Edson] Univ Sao Paulo, Dept Forest Sci, Escola Super Agr Luiz de Queiroz, Ave Padua Dias,11 Cx P 09, BR-13418900 Piracicaba, SP, Brazil. [Klauberg, Carine; Hudak, Andrew Thomas] US Forest Serv, USDA, Rocky Mt Res Stn, Forestry Sci Lab, 1221 South Main St, Moscow, ID 83843 USA. [Silva, Carlos Alberto] Univ Idaho, Dept Nat Resources & Soc, 708 South Deakin St, Moscow, ID 83844 USA. [Bentes, Michelliny de M.] Empresa Brasileira Pesquisa Agr Embrapa, Embrapa Amazonia Oriental CPATU, Trav Dr Eneas Pinheiro S-N,Caixa Postal 48, BR-66095100 Belem, Para, Brazil. RP Klauberg, C (reprint author), Univ Sao Paulo, Dept Forest Sci, Escola Super Agr Luiz de Queiroz, Ave Padua Dias,11 Cx P 09, BR-13418900 Piracicaba, SP, Brazil.; Klauberg, C (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Forestry Sci Lab, 1221 South Main St, Moscow, ID 83843 USA. EM carine_klauberg@hotmail.com RI Vidal, Edson/D-4279-2012 OI Vidal, Edson/0000-0002-8028-6998 FU Foundation for the State of Sao Paulo (FAPESP) [2010/16525-7] FX This study was funded by the Foundation for the State of Sao Paulo (FAPESP), Process No 2010/16525-7. NR 38 TC 0 Z9 0 U1 4 U2 7 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1286-4560 EI 1297-966X J9 ANN FOREST SCI JI Ann. For. Sci. PD SEP PY 2016 VL 73 IS 3 BP 757 EP 764 DI 10.1007/s13595-016-0565-2 PG 8 WC Forestry SC Forestry GA DR9ZA UT WOS:000380253600018 ER PT J AU Szalanski, AL Tripodi, AD Trammel, CE Downey, D AF Szalanski, Allen L. Tripodi, Amber D. Trammel, Clinton E. Downey, Danielle TI Mitochondrial DNA genetic diversity of honey bees, Apis mellifera, in Hawaii SO APIDOLOGIE LA English DT Article DE Apis mellifera; mtDNA; genetic diversity; island biogeography ID UNITED-STATES; APIDAE POPULATIONS; PROTECTED POPULATIONS; NATURAL-POPULATIONS; L. POPULATIONS; SEQUENCE DATA; HYMENOPTERA; AUSTRALIA; MICROSATELLITE; CONSERVATION AB Honey bees, Apis mellifera, in the Hawaiian Islands are geographically isolated from honey bees in mainland United States. We conducted a study on the mitochondrial DNA genetic diversity of honey bees from seven of the Hawaiian Islands by sequencing the intergenic region between the Cytochrome Oxidase I and Cytochrome Oxidase II genes (COI-COII). We observed a total of 10 haplotypes from 235 samples collected from 2009 to 2014. Haplotypes belonged to the A. mellifera C lineage (64 %), M lineage (35 %), and O lineage (1 %). Four of the five C lineage haplotypes found were common among queen breeders in continental United States (C1, C2, C11, C19) and accounted for 99 % of the C lineage bees. Haplotype C33 (1 %) has been observed in feral honey bee populations in continental United States. For the M lineage, which includes the dark honey bee, A. m. mellifera, four haplotypes were observed (M3, M4c"aEuro (TM), M7 and M70), with a novel haplotype unique to Hawaii, M70, being the second most common. Five islands had M lineage haplotypes, with their frequency ranging from 70 % on Maui to 22 % on Molokai. Two individuals of the O lineage, haplotype O1, were found on Oahu. Among the islands, Oahu and Maui, had the greatest amount of haplotypic diversity (haplotypic diversity (H (d) ) = 0.76 and 0.75). Lanai and Kahoolawe had only one haplotype, C1, present. C1 [Szalanski, Allen L.; Tripodi, Amber D.; Trammel, Clinton E.] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. [Tripodi, Amber D.] ARS, USDA, Pollinating Insects Res Unit, Logan, UT 84322 USA. [Downey, Danielle] Hawaii Dept Agr, Hilo, HI 96720 USA. RP Szalanski, AL (reprint author), Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. EM aszalan@uark.edu RI Tripodi, Amber/A-2655-2017 OI Tripodi, Amber/0000-0001-7368-6638 FU University of Arkansas; Arkansas Agricultural Experiment Station; Hawaii Department of Agriculture Apiary Program FX We thank the numerous beekeepers in the state of Hawaii along with Lauren Rusert, Stacey Chun, and Craig Kaneshige for assisting with sample collection. This research was supported in part by the University of Arkansas, Arkansas Agricultural Experiment Station, and the Hawaii Department of Agriculture Apiary Program. NR 46 TC 0 Z9 0 U1 18 U2 55 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD SEP PY 2016 VL 47 IS 5 BP 679 EP 687 DI 10.1007/s13592-015-0416-4 PG 9 WC Entomology SC Entomology GA DS3RM UT WOS:000380700400007 ER PT J AU Katayama, Y Suzuki, T Ebisawa, T Ohtsuka, J Wang, SP Natsume, R Lo, YH Senda, T Nagamine, T Hull, JJ Matsumoto, S Nagasawa, H Nagata, K Tanokura, M AF Katayama, Yukie Suzuki, Tatsuya Ebisawa, Tatsuki Ohtsuka, Jun Wang, Shipeng Natsume, Ryo Lo, Yu-Hua Senda, Toshiya Nagamine, Toshihiro Hull, J. Joe Matsumoto, Shogo Nagasawa, Hiromichi Nagata, Koji Tanokura, Masaru TI A class-A GPCR solubilized under high hydrostatic pressure retains its ligand binding ability SO BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES LA English DT Article DE G protein-coupled receptor; High hydrostatic pressure; Pheromone biosynthesis-activating neuropeptide receptor; Solubilization; Ligand binding ability ID ACTIVATING NEUROPEPTIDE RECEPTOR; PURIFICATION; SILKMOTH AB The effect of high hydrostatic pressure (HHP) on the solubilization of a class-A G protein-coupled receptor, the silkmoth pheromone biosynthesis-activating neuropeptide receptor (PBANR), was investigated. PBANR was expressed in expresSF+ insect cells as a C-terminal fusion protein with EGFP. The membrane fraction was subjected to HHP treatment (200 MPa) at room temperature for 1-16 h in the presence of 0-2.0% (w/v) n-dodecyl-beta-D-maltopyranoside (DDM). The solubilization yield of PBANR-EGFP in the presence of 0.6% (w/v) DDM increased to similar to 1.5-fold after 1 h HHP treatment. Fluorescence-detection size-exclusion chromatography demonstrated that the PBANR-EGFP ligand binding ability was retained after HHP-mediated solubilization. The PBANR-EGFP solubilized with 1.0% DDM under HHP at room temperature for 6 h retained ligand binding ability, whereas solubilization in the absence of HHP treatment resulted in denaturation. (C) 2016 Elsevier B.V. All rights reserved. C1 [Katayama, Yukie; Suzuki, Tatsuya; Ebisawa, Tatsuki; Ohtsuka, Jun; Wang, Shipeng; Nagasawa, Hiromichi; Nagata, Koji; Tanokura, Masaru] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan. [Natsume, Ryo] Tokyo Denki Univ, Sch Engn, Dept Green & Sustainable Chem, Adachi Ku, 5 Senju Asahi Cho, Tokyo 1208551, Japan. [Lo, Yu-Hua; Senda, Toshiya] High Energy Accelerator Res Org KEK, Inst Mat Struct Sci, Photon Factory, Struct Biol Res Ctr, 1-1 Oho, Tsukuba, Ibaraki 3050801, Japan. [Nagamine, Toshihiro; Matsumoto, Shogo] RIKEN, Mol Entomol Lab, Adv Sci Inst, Wako, Saitama 3510198, Japan. [Hull, J. Joe] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Nagasawa, Hiromichi] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China. RP Nagata, K; Tanokura, M (reprint author), Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan. EM aknagata@mail.ecc.u-tokyo.ac.jp; amtanok@mail.ecc.u-tokyo.ac.jp FU JSPS KAKENHI Grant [17681026]; Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan; Japan Agency for Medical Research and Development (AMED) FX This work was supported in part by JSPS KAKENHI Grant Number 17681026 and the Targeted Proteins Research Program from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and by the Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan and Japan Agency for Medical Research and Development (AMED). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 17 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0005-2736 EI 0006-3002 J9 BBA-BIOMEMBRANES JI Biochim. Biophys. Acta-Biomembr. PD SEP PY 2016 VL 1858 IS 9 BP 2145 EP 2151 DI 10.1016/j.bbamem.2016.06.012 PG 7 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DS2LR UT WOS:000380601500022 PM 27342372 ER PT J AU Rojas, MG Morales-Ramos, JA Riddick, EW AF Rojas, M. Guadalupe Morales-Ramos, Juan A. Riddick, Eric W. TI Use of Tenebrio molitor (Coleoptera: Tenebrionidae) powder to enhance artificial diet formulations for Coleomegilla maculata (Coleoptera: Coccinellidae) SO BIOLOGICAL CONTROL LA English DT Article DE Ladybird beetles; Mass rearing; In vitro rearing; Development; Fecundity; Life cycle; Life table ID POTENTIAL UTILIZATION; LADY BEETLE; CORN POLLEN; REPRODUCTION; SUITABILITY; PARAMETERS; EGGS; PREY; FOOD AB The predatory lady beetle Coleomegilla maculata has potential to control several arthropod pests on crop plants in greenhouses and high tunnels. However, an effective artificial diet is needed in order to mass produce C. maculata in sufficient quantities for augmentative releases. The objectives of this study were to develop a semi-solid insect-free artificial diet, evaluate the diet effects on C. maculata fitness, and determine if adding extracts (consisting of dry powder of whole pupae) of the yellow mealworm, Tenebrio molitor, could improve the suitability of the diet formulations and the life parameters of C. maculata. Although C. maculata completed development and reproduced on two meridic artificial diet formulations (M1 and M2), neither one was as effective as a control diet mix consisting of Ephestia kuehniella, Artemia sp. eggs, and bee pollen. Incorporation of T. molitor extracts into the diet formulation (5 and 7% in diets T1 and T2, respectively), significantly improved C. maculata larval survival, shortened post-embryonic development time, increased fecundity and egg viability as compared to the meridic diet formulations. Adults feeding on diets T1 and T2 (containing T. molitor extracts) produced more eggs and had a better survival than those feeding on the control diet mix. Also, egg viability was significantly higher in diets T1 and T2 than in the control. Demographic parameters showed that the overall fitness of C. maculata fed the meridic diet containing T. molitor extracts was superior to beetles fed the control mix. The role of feeding stimulation by T. molitor added components as responsible for the diet improvement is discussed. Published by Elsevier Inc. C1 [Rojas, M. Guadalupe; Morales-Ramos, Juan A.; Riddick, Eric W.] ARS, USDA, Natl Biol Control Lab, Stoneville, MS 38776 USA. RP Morales-Ramos, JA (reprint author), ARS, USDA, Natl Biol Control Lab, Stoneville, MS 38776 USA. EM juan.moralesramos@ars.usda.gov OI riddick, eric/0000-0002-4795-961X NR 44 TC 0 Z9 0 U1 8 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD SEP PY 2016 VL 100 BP 70 EP 78 DI 10.1016/j.biocontrol.2016.05.018 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DS0FB UT WOS:000380269700010 ER PT J AU Hogg, BN Moran, PJ Smith, L AF Hogg, Brian N. Moran, Patrick J. Smith, Lincoln TI Multi-generational impacts of the psyllid Arytinnis hakani (Hemiptera: Psyllidae) on growth and survival of the invasive weed Genista monspessulana SO BIOLOGICAL CONTROL LA English DT Article DE Agent efficacy; Arytinnis; French broom; Genista; Psyllidae; Weed control ID BIOLOGICAL-CONTROL AGENTS; EFFICACY ASSESSMENT; INTRODUCED INSECTS; REPRODUCTION; HERBIVORES; QUARANTINE; HOMOPTERA; PLANTS AB Pre-release efficacy assessments of biological control agents can identify species with the most potential to impact the target weed. Experiments typically occur within a single generation of the agent, however, and strong impacts on target weeds may take longer to emerge. This study examined the effects of the prospective agent Arytinnis hakani (Hemiptera: Psyllidae) on the invasive weed French broom, Genista monspessulana, over three generations of the psyllid (similar to 150 days) under quarantine conditions. All of the psyllid-free control plants survived to the end of the experiment, whereas 23 out of 28 (82.1%) of the plants infested with psyllids died. Only three plants with psyllids died during the first psyllid generation; plant mortality rate peaked at the transition between the second and third psyllid generations. Plant growth was also reduced on plants with psyllids. Impacts on proportional increases in plant height and total stem length were evident by the end of the first psyllid generation, but effects on proportional change in leaf number were not apparent until the end of the third psyllid generation. By the end of the experiment, average proportional increases in height, total stem length and leaf number were 65.7%, 60.7% and 53.2% lower, respectively, on surviving psyllid-infested plants than controls. Final above-ground biomass of surviving psyllid-infested plants was 66.4% lower on average compared to the psyllid-free control plants. The results of this study highlight the need for pre-release efficacy assessments that allow effects on target weeds to develop over time. Published by Elsevier Inc. C1 [Hogg, Brian N.; Moran, Patrick J.; Smith, Lincoln] ARS, USDA, Exot & Invas Weeds Res Unit, 800 Buchanan St, Albany, CA USA. RP Hogg, BN (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM brian.hogg@ars.usda.gov; patrick.moran@ars.usda.gov; link.smith@ars.usda.gov NR 49 TC 0 Z9 0 U1 5 U2 12 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD SEP PY 2016 VL 100 BP 87 EP 93 DI 10.1016/j.biocontrol.2016.05.015 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DS0FB UT WOS:000380269700012 ER PT J AU Weber, JG Key, N O'Donoghue, E AF Weber, Jeremy G. Key, Nigel O'Donoghue, Erik TI Does Federal Crop Insurance Make Environmental Externalities from Agriculture Worse? SO JOURNAL OF THE ASSOCIATION OF ENVIRONMENTAL AND RESOURCE ECONOMISTS LA English DT Article DE Agriculture; Crop insurance; Environmental externalities; Fertilizer ID US CORN-BELT; MORAL HAZARD; ACREAGE DECISIONS; NITRATE-NITROGEN; CHEMICAL USE; PANEL-DATA; RISK; DIVERSIFICATION; PARTICIPATION; PROGRAMS AB Farmers dramatically increased their use of federal crop insurance in the 2000s. From 2000 to 2013, premium subsidies increased sevenfold and acres enrolled increased by 77%. Although designed for nonenvironmental goals, subsidized insurance may affect the use of land, fertilizer, and agrochemicals and, therefore, environmental externalities from agriculture. Using novel panel data, we examine farmer responses to changes in coverage with an empirical approach that exploits program limits on coverage that were more binding for some farmers than for others. Estimates indicate that expanded coverage had little effect on the share of farmland harvested, crop specialization, productivity, or fertilizer and chemical use. More broadly, we construct and describe a new nationwide, farm-level panel data set with nearly 32,500 farms observed at least twice over the 2000-2013 period, a resource that should enrich US agro-environmental research. C1 [Weber, Jeremy G.] Univ Pittsburgh, Grad Sch Publ & Int Affairs, 3601 Wesley W Posvar Hall, Pittsburgh, PA 15260 USA. [Weber, Jeremy G.] Univ Pittsburgh, Dept Econ, 3601 Wesley W Posvar Hall, Pittsburgh, PA 15260 USA. [Key, Nigel; O'Donoghue, Erik] ERS, USDA, Washington, DC USA. RP Weber, JG (reprint author), Univ Pittsburgh, Grad Sch Publ & Int Affairs, 3601 Wesley W Posvar Hall, Pittsburgh, PA 15260 USA.; Weber, JG (reprint author), Univ Pittsburgh, Dept Econ, 3601 Wesley W Posvar Hall, Pittsburgh, PA 15260 USA. EM jgw99@pitt.edu; nkey@ers.usda.gov; eodonoghue@ers.usda.gov NR 47 TC 0 Z9 0 U1 5 U2 5 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 2333-5955 EI 2333-5963 J9 J ASSOC ENVIRON RESO JI J. Assoc. Environ. Resour. Econ. PD SEP PY 2016 VL 3 IS 3 BP 707 EP 742 DI 10.1086/687549 PG 36 WC Economics SC Business & Economics GA DS1MT UT WOS:000380360600006 ER PT J AU Zhu, JWJ Zhang, QH Taylor, DB Friesen, KA AF Zhu, Junwei J. Zhang, Qing-he Taylor, David B. Friesen, Kristina A. TI Visual and olfactory enhancement of stable fly trapping SO PEST MANAGEMENT SCIENCE LA English DT Article DE Stomoxys calcitrans; visual response; attractant; enhanced trapping ID FLIES DIPTERA MUSCIDAE; STOMOXYS-CALCITRANS DIPTERA; BEHAVIORAL-RESPONSES; CATTLE FEEDLOTS; TREATED TARGETS; SPP. DIPTERA; STICKY TRAPS; FIELD; ATTRACTANTS; POPULATIONS AB BACKGROUNDStable flies are considered to be one of the major blood-feeding pests in the US livestock industry, causing losses running into billions of dollars annually. Adult stable flies are highly attracted to Alsynite traps; however, Alsynite is becoming increasingly difficult to obtain and is expensive. RESULTSHere, we report on the development of a less expensive and more efficacious trap based upon a white panel with the option to add visual and olfactory stimuli for enhanced stable fly trapping. White panel traps caught twice as many stable flies than Alsynite traps. Baiting the traps with synthetic manure volatiles increased catches 2-3-fold. Electroretinographic recordings of stable flies showed strong peaks of visual sensitivities occurring at 330-360 nm, 460-525 nm and 605-635 nm. A laboratory study indicated that young stable flies are more responsive to white, whereas gravid females prefer blue; in the field, white traps caught more stable flies than patterned or blue-black traps. CONCLUSIONStable fly control can be enhanced by developing more efficient trapping systems with added visual and olfactory stimuli. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Zhu, Junwei J.; Taylor, David B.; Friesen, Kristina A.] Univ Nebraska, USDA ARS, USDA ARS Agroecosyst Management Res Unit, 305B Entomol Hall, Lincoln, NE 68583 USA. [Zhang, Qing-he] Sterling Int Inc, Spokane, WA 99216 USA. RP Zhu, JWJ (reprint author), Univ Nebraska, USDA ARS, AMRU, 305B Entomol Hall, Lincoln, NE 68583 USA. EM Jerry.Zhu@ars.usda.gov NR 36 TC 0 Z9 0 U1 7 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD SEP PY 2016 VL 72 IS 9 BP 1765 EP 1771 DI 10.1002/ps.4207 PG 7 WC Agronomy; Entomology SC Agriculture; Entomology GA DS4CQ UT WOS:000380729400016 PM 26662853 ER PT J AU Engeman, RM Orzell, SL Felix, RK Tillman, EA Killian, G Avery, ML AF Engeman, Richard M. Orzell, Steve L. Felix, Rodney K. Tillman, Eric A. Killian, Gary Avery, Michael L. TI Feral swine damage to globally imperiled wetland plant communities in a significant biodiversity hotspot in Florida SO BIODIVERSITY AND CONSERVATION LA English DT Article DE Animal damage; Biodiversity; Endemic and endangered plants; Invasive species; Seepage slopes; Sus scrofa ID NINO-SOUTHERN-OSCILLATION; FOREST; MANAGEMENT; DISTURBANCES; CONSERVATION; POPULATION; VEGETATION; MILITARY; IMPACTS; HABITAT AB We studied rooting damage during five-years of feral swine control at Avon Park Air Force Range, a significant botanical biodiversity hotspot in peninsular Florida with many globally imperiled plant species and communities. While control reduced swine abundance, remaining animals consistently rooted the 49 studied sites in both middle-dry season (MDS) and late-dry season (LDS) each year. At each study site, we measured rooting with sub-meter accuracy. Neither total nor proportional area rooted differed in either season, across study years, or among plant community types: herbaceous seepage slopes, wet pine savannas, wet grasslands. The proportion of sites with damage during MDS was at least 25 % less than pre-control baseline. During LDS, the proportion of sites with damage increased over years but remained below the initial 2 years' MDS results. Fresh rooting frequency (rooting < 1 week-old) across sites dropped precipitously from baseline and remained low for MDS. Fresh rooting frequency among sites during LDS was lower than MDS for all but year two of the study. Canopied habitat < 50 m from a study site almost guaranteed rooting at the site each observation period. We propose actions for protecting wetlands, integrating swine control into other compatible land use practices, and improving swine control efficacy. While we measured damage amounts at each site very accurately, our approach of also considering frequency of rooting and frequency of fresh rooting across sites offers low-labor means to broadly assess swine damage and control efficacy at large geographic scales because in-field measurements of damage amounts are not needed. C1 [Engeman, Richard M.; Killian, Gary] USDA APHIS WS Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Orzell, Steve L.] Avon Pk Air Force Range, 29 South Blvd, Avon Pk Afr, FL 33825 USA. [Felix, Rodney K.; Tillman, Eric A.; Avery, Michael L.] USDA APHIS WS Natl Wildlife Res Ctr, Florida Field Stn, 2820 E Univ Blvd, Gainesville, FL 32641 USA. [Felix, Rodney K.] Eglin AFB Nat Resources Wildlife, 107 SR 85 North, Niceville, FL 32578 USA. RP Engeman, RM (reprint author), USDA APHIS WS Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM richard.m.engeman@aphis.usda.gov FU National Wildlife Research Center [08-7483-0707(IA)]; Avon Park Air Force Range; APAFR; USAF FX We appreciate the support of Paul Ebersbach, Chief, Environmental Flight, APAFR, and the USAF for funding this project. For field assistance, we are grateful to J. S. Humphrey, S. Koontz, W. E. Bruce, K. L. Keacher, and E. Fetzer. We appreciate the GIS support provided by Casey Lindemuth for assembling spatial data used extensively in this study. This research was supported through interagency agreement 08-7483-0707(IA) between the National Wildlife Research Center and Avon Park Air Force Range. NR 61 TC 0 Z9 0 U1 40 U2 40 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3115 EI 1572-9710 J9 BIODIVERS CONSERV JI Biodivers. Conserv. PD SEP PY 2016 VL 25 IS 10 BP 1879 EP 1898 DI 10.1007/s10531-016-1166-y PG 20 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7WX UT WOS:000380111500005 ER PT J AU Asadi, A Miller, M Sultana, S Moon, RJ Kalaitzidou, K AF Asadi, Amir Miller, Mark Sultana, Sanzida Moon, Robert J. Kalaitzidou, Kyriaki TI Introducing cellulose nanocrystals in sheet molding compounds (SMC) SO COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING LA English DT Article DE Cellulose; Polymer-matrix composites (PMCs); Mechanical properties; Moulding compounds ID MECHANICAL-PROPERTIES; EPOXY COMPOSITES; GLASS-FIBER; NANOCOMPOSITES; BEHAVIOR; SUSPENSIONS; INTERFACES; NANOFIBER; ADHESION; NETWORK AB The mechanical properties of short glass fiber/epoxy composites containing cellulose nanocrystals (CNC) made using sheet molding compound (SMC) manufacturing method as well as the rheological and thermomechanical properties of the CNC-epoxy composites were investigated as a function of the CNC content. CNC up to 1.4 wt% were dispersed in the epoxy to produce the resin for SMC production. The addition of CNC in the resin increased its viscosity and slightly reduced the heat of reaction during the polymerization without altering the curing time and temperature and the effective pot life of the resin. The incorporation of 0.9 wt% CNC in the SMC composite resulted in increases in elastic modulus and tensile strength by similar to 25% and similar to 30% and in flexural modulus and strength by similar to 44% and similar to 33% respectively. Concentrations of CNC up to 0.9 wt% in the SMC composite did not alter the impact energy. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Asadi, Amir; Miller, Mark; Sultana, Sanzida; Kalaitzidou, Kyriaki] Georgia Inst Technol, GW Woodruff Sch Mech Engn, Georgia Tech Mfg Inst, Atlanta, GA 30332 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Moon, Robert J.; Kalaitzidou, Kyriaki] Georgia Inst Technol, Sch Mat Sci & Engn, Atlanta, GA 30332 USA. RP Kalaitzidou, K (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, 813 Ferst Dr, Atlanta, GA 30332 USA. EM kyriaki.kalaitzidou@me.gatech.edu FU P3 Nano; U.S. Endowment for Forestry and Communities FX This work was supported by funding from P3 Nano and the U.S. Endowment for Forestry and Communities. The authors would like to thank Prof. Jon Colton for providing the mechanical testing equipment. The authors gratefully acknowledge the donation of glass fibers by Owens Corning. NR 37 TC 1 Z9 1 U1 9 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1359-835X EI 1878-5840 J9 COMPOS PART A-APPL S JI Compos. Pt. A-Appl. Sci. Manuf. PD SEP PY 2016 VL 88 BP 206 EP 215 DI 10.1016/j.compositesa.2016.05.033 PG 10 WC Engineering, Manufacturing; Materials Science, Composites SC Engineering; Materials Science GA DR7LQ UT WOS:000380081600024 ER PT J AU Qiu, ZY Dosskey, MG Kang, Y AF Qiu, Zeyuan Dosskey, Michael G. Kang, Yang TI Choosing between alternative placement strategies for conservation buffers using Borda count SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Multi-criteria decision-making; Fuzzy logic; Borda count; Analytic hierarchy process; Conservation buffer placement ID DECISION-MAKING; WATER-QUALITY; UNITED-STATES; MANAGEMENT; LAND; HABITATS; APPROVAL; CRITERIA AB There is inherent ambiguity when comparing decision criteria in multi-criteria decision-making (MCDM). A fuzzy Borda count was developed to take into account some of the ambiguity and derive criteria weights by linguistically comparing decision criteria. This study extends the fuzzy Borda count to take into account agents' confidence in their preferences for criteria weights. The procedure is applied to prioritize agricultural lands for conservation buffer placement using multiple criteria in the Raritan River Basin in New Jersey. These criteria, which include soil erodibility, hydrological sensitivity, wildlife habitat, and impervious surface, capture the conservation buffers' ecosystem services in terms of reducing soil erosion, controlling runoff generation, enhancing wildlife habitat, and mitigating stormwater impacts, respectively. A survey was conducted of conservation professionals including federal employees at NRCS, state and local agencies and nongovernmental environmental organizations to elicit agents' preferences for multiple benefits of conservation buffers using a fuzzy pairwise comparison method. The study compares the fuzzy MCDM procedure to a class-based MCDM procedure for prioritizing agricultural lands for conservation buffer placement. The comparative results show that both procedures have their advantages and disadvantages, but generate comparable prioritization results. Further research is needed to improve the proposed fuzzy MCDM procedure to handle missing values in eliciting agents' preferences for comparing multiple criteria. (C) 2016 Elsevier B.V. All rights reserved. C1 [Qiu, Zeyuan] New Jersey Inst Technol, Dept Chem & Environm Sci, Newark, NJ 07102 USA. [Dosskey, Michael G.] US Forest Serv, USDA, Natl Agroforestry Ctr, Lincoln, NE USA. [Kang, Yang] Columbia Univ, Dept Stat, New York, NY USA. RP Qiu, ZY (reprint author), New Jersey Inst Technol, Dept Chem & Environm Sci, Newark, NJ 07102 USA. EM zeyuan.qiu@njit.edu FU U.S. Department of Agriculture; National Institute of Food and Agriculture [2012-67019-19348]; Natural Resources Conservation Service [692B26104]; Forest Service, Southern Research Station, National Agroforestry Center [09-DG-11330152-057] FX The funding support to this study was partially provided by the U.S. Department of Agriculture, the National Institute of Food and Agriculture (grant number 2012-67019-19348), Natural Resources Conservation Service (grant number 692B26104), and Forest Service, Southern Research Station, National Agroforestry Center (grant number 09-DG-11330152-057) to New Jersey Institute of Technology. The authors are grateful to three anonymous reviewers for their helpful comments in improving the overall quality of the manuscript. NR 53 TC 1 Z9 1 U1 12 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD SEP PY 2016 VL 153 BP 66 EP 73 DI 10.1016/j.landurbplan.2016.03.012 PG 8 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA DR1ZZ UT WOS:000379705700006 ER PT J AU Kim, D Ximenes, EA Nichols, NN Cao, GL Frazer, SE Ladisch, MR AF Kim, Daehwan Ximenes, Eduardo A. Nichols, Nancy N. Cao, Guangli Frazer, Sarah E. Ladisch, Michael R. TI Maleic acid treatment of biologically detoxified corn stover liquor SO BIORESOURCE TECHNOLOGY LA English DT Article DE Bioabatement; Inhibitors; Hemicellulase supplementation; Enzymatic hydrolysis; Liquid hot water pretreatment; Fermentation; Ethanol; Maleic acid ID HOT-WATER PRETREATMENT; ENZYMATIC-HYDROLYSIS; SACCHAROMYCES-CEREVISIAE; FERMENTATION; INHIBITION; CELLULOSE; HARDWOOD; PHENOLS; BIOMASS; SUPPLEMENTATION AB Elimination of microbial and enzyme inhibitors from pretreated lignocellulose is critical for effective cellulose conversion and yeast fermentation of liquid hot water ( LHW) pretreated corn stover. In this study, xylan oligomers were hydrolyzed using either maleic acid or hemicellulases, and other soluble inhibitors were eliminated by biological detoxification. Corn stover at 20% ( w/v) solids was LHW pretreated LHW ( severity factor: 4.3). The 20% solids ( w/v) pretreated corn stover derived liquor was recovered and biologically detoxified using the fungus Coniochaeta ligniaria NRRL30616. After maleic acid treatment, and using 5 filter paper units of cellulase/g glucan ( 8.3 mg protein/g glucan), 73% higher cellulose conversion from corn stover was obtained for biodetoxified samples compared to undetoxified samples. This corresponded to 87% cellulose to glucose conversion. Ethanol production by yeast of pretreated corn stover solids hydrolysate was 1.4 times higher than undetoxified samples, with a reduction of 3 h in the fermentation lag phase. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Kim, Daehwan; Ximenes, Eduardo A.; Ladisch, Michael R.] Purdue Univ, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA. [Kim, Daehwan; Ximenes, Eduardo A.; Ladisch, Michael R.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. [Ladisch, Michael R.] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA. [Nichols, Nancy N.; Frazer, Sarah E.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Cao, Guangli] Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China. [Cao, Guangli] Harbin Inst Technol, State Key Lab Urban Water Resource & Environm, Harbin 150001, Peoples R China. RP Ladisch, MR (reprint author), Purdue Univ, Renewable Resources Engn Lab, W Lafayette, IN 47907 USA. EM ladisch@purdue.edu FU Hatch Act [10677, 10646]; Purdue University Agricultural Research Programs; Department of Agricultural and Biological Engineering FX This work is supported by Hatch Act 10677, 10646, Purdue University Agricultural Research Programs, and the Department of Agricultural and Biological Engineering. We are grateful to Xingya (Linda) Li at LORRE for excellent analysis assistance and Bruce Dien, Carla Carie and Neal Hengge for internal review of this manuscript. NR 37 TC 0 Z9 0 U1 8 U2 10 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD SEP PY 2016 VL 216 BP 437 EP 445 DI 10.1016/j.biortech.2016.05.086 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DQ9UX UT WOS:000379555900055 PM 27262718 ER PT J AU Kim, SM Dien, BS Tumbleson, ME Rausch, KD Singh, V AF Kim, Sun Min Dien, Bruce S. Tumbleson, M. E. Rausch, Kent D. Singh, Vijay TI Improvement of sugar yields from corn stover using sequential hot water pretreatment and disk milling SO BIORESOURCE TECHNOLOGY LA English DT Article DE Hot water pretreatment; Dilute acid pretreatment; Disk milling; Mechanical grinding; Corn stover ID SULFURIC-ACID PRETREATMENT; ENZYMATIC-HYDROLYSIS; ETHANOL-PRODUCTION; CELLULOSIC ETHANOL; RICE STRAW; SEVERITY; FRACTIONATION; BIOCONVERSION; TECHNOLOGIES; CONVERSION AB Efficient pretreatment is essential for economic conversion of lignocellulosic feedstocks into monosaccharides for biofuel production. To realize high sugar yields with low inhibitor concentrations, hot water or dilute acid pretreatment followed by disk milling is proposed. Corn stover at 20% solids was pretreated with hot water at 160-200 degrees C for 4-8 min with and without subsequent milling. Hot water pretreatment and disk milling acted synergistically to improve glucose and xylose yields by 89% and 134%, respectively, compared to hot water pretreatment alone. Hot water pretreated (180 degrees C for 4 min) and milled samples had the highest glucose and xylose yields among all hot water pretreated and milled samples, which were comparable to samples pretreated with 0.55% dilute acid at 160 degrees C for 4 min. However, samples pretreated with 1% dilute acid at 150 degrees C for 4 min and disk milled had the highest observed glucose (87.3%) and xylose yields (83.4%). (C) 2016 The Authors. Published by Elsevier Ltd. C1 [Kim, Sun Min; Tumbleson, M. E.; Rausch, Kent D.; Singh, Vijay] Univ Illinois, Dept Agr & Biol Engn, 1304 West Penn Ave, Urbana, IL 61801 USA. [Dien, Bruce S.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Singh, V (reprint author), Univ Illinois, Dept Agr & Biol Engn, 1304 West Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu OI Dien, Bruce/0000-0003-3863-6664 FU DSM Biobased Products & Services (Elgin, IL, US) FX We would like to acknowledge DSM Biobased Products & Services (Elgin, IL, US) for funding support and donation of cellulases enzyme preparation. NR 36 TC 0 Z9 0 U1 8 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD SEP PY 2016 VL 216 BP 706 EP 713 DI 10.1016/j.biortech.2016.06.003 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DQ9UX UT WOS:000379555900088 PM 27289063 ER PT J AU Williams, CJ Pierson, FB Kormos, PR Al-Hamdan, OZ Hardegree, SP Clark, PE AF Williams, C. Jason Pierson, Frederick B. Kormos, Patrick R. Al-Hamdan, Osama Z. Hardegree, Stuart P. Clark, Patrick E. TI Ecohydrologic response and recovery of a semi-arid shrubland over a five year period following burning SO CATENA LA English DT Article DE Erosion; Fire; Hydrologic recovery; Infiltration; Runoff; Soil water repellency; Hydrophobicity; Prescribed fire; Rangeland; Sagebrush steppe ID SOIL-WATER REPELLENCY; STORM KING MOUNTAIN; SAGEBRUSH-STEPPE; OVERLAND-FLOW; FIRE REGIMES; WESTERN US; DEBRIS FLOWS; HYDROLOGIC VULNERABILITY; JUNIPER ENCROACHMENT; RAINFALL SIMULATION AB Increasing trends in wildfire activity on semi-arid rangelands necessitate advancement in understanding of fire impacts on vegetation, soils, and runoff and erosion processes. This study used artificially applied rainfall and concentrated overland flow experiments to evaluate the ecohydrologic response and recovery of a semi-arid shrubland in the Great Basin Region, USA, following fire. Rainfall experiments were conducted at the 0.5 m(2) plot scale to assess fire impacts on rainsplash and sheetfiow processes. Concentrated flow experiments were applied on 9 m(2) plots to evaluate fire impacts on concentrated overland flow processes. Vegetation, soil, hydrologic, and erosion variables were assessed at each scale pre-fite and 1, 2, and 5 yr post-fire. Infiltration and runoff on rainfall simulation plots were affected more by measured background soil water repellency than fire effects on vegetation and soils. Runoff from rainfall on shrub-dominated plots was unchanged 1 yr post-fire, but runoff from interspace plots between shrubs declined 1 yr post-fire. Runoff increased on shrub and interspace rainfall plots 2 yr post-fire and then declined in the 5 yr post-fire. Bare ground generally declined across study years, implicating the temporal variability in soil water repellency as the causal factor for infiltration and runoff trends. Erosion on rainfall plots increased by factors of 8 to more than 10 following fire removal of vegetation and ground cover and declined with vegetation recovery through five growing seasons. Concentrated overland flow plots generated slightly more total runoff and 26-fold-more total sediment 1 yr following burning relative to pre-fire measures. Erosion from concentrated overland flow remained greater on burned than unburned plots after five growing seasons even though ground cover returned to approximately 85%. The relative recovery of vegetation and total ground cover were typical for the shrubland community assessed, but elevated erosion with 85% ground cover 5 yr post-fire was unexpected. The persistent high sediment delivery from concentrated plots is attributed to the fine textured soils and thin litter accumulation. The importance of considering erodibility in context with sediment supply and vegetative recovery is discussed. The results demonstrate the complexity of post fire ecohydrologic interactions, advance process understanding of post-fire ecohydrologic responses for semiarid rangelands, and underscore the need for additional studies on post-fire recovery over time. Published by Elsevier B.V. C1 [Williams, C. Jason; Pierson, Frederick B.; Kormos, Patrick R.; Al-Hamdan, Osama Z.; Hardegree, Stuart P.; Clark, Patrick E.] USDA ARS, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. [Al-Hamdan, Osama Z.] Univ Idaho, Dept Biol & Agr Engn, Moscow, ID 83844 USA. [Al-Hamdan, Osama Z.] Texas A&M Univ Kingsville, Dept Civil & Architectural Engn, Kingsville, TX 78363 USA. RP Williams, CJ (reprint author), USDA ARS, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. EM jason.williams@ars.usda.gov NR 113 TC 0 Z9 0 U1 12 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD SEP PY 2016 VL 144 BP 163 EP 176 DI 10.1016/j.catena.2016.05.006 PG 14 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DQ7GW UT WOS:000379375900016 ER PT J AU Crocker, SJ Liknes, GC Mckee, FR Albers, JS Aukema, BH AF Crocker, Susan J. Liknes, Greg C. McKee, Fraser R. Albers, Jana S. Aukema, Brian H. TI Stand-level factors associated with resurging mortality from eastern larch beetle (Dendroctonus simplex LeConte) SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Dendroctonus simplex; Eastern larch beetle; Larix laricina; Eastern larch; Tamarack; Forest inventory ID MOUNTAIN PINE-BEETLE; UNITED-STATES; CLIMATE-CHANGE; BIOTIC DISTURBANCES; BARK BEETLES; SCOLYTIDAE; COLEOPTERA; FORESTS; NEWFOUNDLAND; OUTBREAK AB The current outbreak of eastern larch beetle (Dendroctonus simplex LeConte) in Minnesota, USA, ongoing since 2000, has been characterized with a severity and duration unprecedented in previous outbreaks within the state. This study investigates the relationship between tamarack mortality due to eastern larch beetle and extant stand and site characteristics. Observations of tree attribute information and site condition collected over the course of the outbreak from nearly 15,000 tamarack trees in three different ecological regions of Minnesota were modeled using linear regression. Increasing tree diameter was the best indicator of tamarack mortality within the northern limits of the state's tamarack range and during the later portion of the outbreak. Tamarack density was negatively related to tree mortality in the north central region during the later outbreak, while the density of co-located non-host gymnosperms was positively related to tree mortality in areas of marginal host distribution. A temporal change in the importance of predictor variables suggests that the influence of tree and site characteristics evolved as the outbreak progressed and subsequent mortality increased. A greater understanding of the factors associated with tamarack mortality and how the those factors change over time and space will help to inform management practices and mitigate the impacts of this bark beetle on the tamarack resource in Minnesota. Published by Elsevier B.V. C1 [Crocker, Susan J.; Liknes, Greg C.] USDA, Forest Serv, No Res Stn, Forest Inventory & Anal, 1992 Folwell Ave, St Paul, MN 55108 USA. [McKee, Fraser R.; Aukema, Brian H.] Univ Minnesota, Dept Entomol, 1980 Folwell Ave, St Paul, MN 55108 USA. [Albers, Jana S.] Minnesota Dept Nat Resources, Div Forestry, 1201 E Hwy 2, Grand Rapids, MN 55744 USA. [McKee, Fraser R.] Govt Alberta, Minist Agr & Forestry, POB 450,9503 Beaverhill Rd, Lac La Biche, AB T0A 2CO, Canada. RP Crocker, SJ (reprint author), USDA, Forest Serv, No Res Stn, Forest Inventory & Anal, 1992 Folwell Ave, St Paul, MN 55108 USA. EM scrocker@fs.fed.us NR 54 TC 0 Z9 0 U1 13 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD SEP 1 PY 2016 VL 375 BP 27 EP 34 DI 10.1016/j.foreco.2016.05.016 PG 8 WC Forestry SC Forestry GA DR4QI UT WOS:000379886900003 ER PT J AU Looney, CE D'Amato, AW Fraver, S Palik, BJ Reinikainen, MR AF Looney, Christopher E. D'Amato, Anthony W. Fraver, Shawn Palik, Brian J. Reinikainen, Michael R. TI Examining the influences of tree-to-tree competition and climate on size-growth relationships in hydric, multi-aged Fraxinus nigra stands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Competition; Size-growth relationship; Fraxinus nigra; Wetland forest; Climate ID EMERALD ASH BORER; ASYMMETRIC COMPETITION; NORTHERN MINNESOTA; LOCAL COMPETITION; FOREST; DOMINANCE; DYNAMICS; FLOODPLAIN; MORTALITY; PATTERNS AB Most research on tree-tree competition and size-growth relationship (SGR a stand-level metric that infers the relative efficiency with which different sized trees utilize available resources) has focused on upland systems. It is unclear if inferences from these studies extend to wetland forests. Moreover, no study to date has thoroughly investigated the relationship between individual tree-tree competition and SGR. To fill these research gaps, we conducted a dendrochronological study examining the relationship of tree-tree competition, SGR, and climate in late-successional Fraxinus nigra (black ash) wetland forests in northern Minnesota, USA. We took advantage of a detailed, stem-mapped dataset of 1670 trees in five late-successional, multi-aged stands to explore the following research questions: (1) how do competitive interactions, particularly size symmetry, influence individual-tree growth; (2) do late-successional F. nigra stands display inverse asymmetric SGR; and (3) do short-term variations in drought influence SGR in F. nigra wetland forests? Using neighborhood competition indices, which characterize the growth of individual trees based on the size, number, and distance of competitors, we examined the nature and strength of individual tree-tree interactions. Additionally, we used SGR to determine how tree size and individual tree contributions to stand productivity relate to changes in stand growth and competitive interactions during stand development. At the individual tree level, we found evidence of size asymmetric competition, with larger trees disproportionately suppressing the growth of smaller trees. However, tree size was a stronger predictor of growth than competition at all sites. At the stand level, our multi-aged F. nigra sites showed consistent patterns of inverse size-asymmetric SGR (i.e., smaller individual trees growing at disproportionately higher rates relative to larger trees), which is generally consistent with previous observations of mature upland forests and supports the hypothesis that large trees decline in relative growth as stands age. While seemingly counter-intuitive, the simultaneous presence of size-asymmetric individual tree-level competition and stand-level inverse asymmetric SGR suggests declines in large tree production efficiency. Drought effects on SGR, as expressed by PDSI, while sometimes evident, appeared weak on both relatively mesic and extremely wet sites. Our findings, which are consistent with previous studies of both F. nigra wetlands and upland forests, demonstrate that the combined results of individual-tree competition models and stand-level SGR can provide deeper insights into growth and competition in F. nigra and other forest types. (C) 2016 Elsevier B.V. All rights reserved. C1 [Looney, Christopher E.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05095 USA. [Fraver, Shawn] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Palik, Brian J.] USDA, No Res Stn, Forest Serv, Grand Rapids, MN 55744 USA. [Reinikainen, Michael R.] Univ Minnesota, Cloquet Forestry Ctr, Cloquet, MN 55720 USA. RP Looney, CE (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM loone016@umn.edu; awdamato@uvm.edu; shawn.fraver@maine.edu; bpalik@fs.fed.us; mike.reinikainen@gmail.com FU Minnesota Environmental and Natural Resources Trust Fund; Frederick and Philip Noel Knorr Fellowship through the University of Minnesota, Department of Forest Resources; Northwest Paper Foundation Fellowship through the University of Minnesota, Department of Forest Resources; USDA Forest Service, Northern Research Station FX Funding for this study was provided by the Minnesota Environmental and Natural Resources Trust Fund; the Frederick and Philip Noel Knorr and Northwest Paper Foundation Fellowships through the University of Minnesota, Department of Forest Resources; and the USDA Forest Service, Northern Research Station. We are grateful to the Chippewa National Forest for providing logistical support and Kyle Gill, Paul Klockow, and Nick Jensen for assistance with field sampling. NR 67 TC 0 Z9 0 U1 20 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD SEP 1 PY 2016 VL 375 BP 238 EP 248 DI 10.1016/j.foreco.2016.05.050 PG 11 WC Forestry SC Forestry GA DR4QI UT WOS:000379886900024 ER PT J AU Carey, MP Sethi, SA Larsen, SJ Rich, CF AF Carey, Michael P. Sethi, Suresh A. Larsen, Sabrina J. Rich, Cecil F. TI A primer on potential impacts, management priorities, and future directions for Elodea spp. in high latitude systems: learning from the Alaskan experience SO HYDROBIOLOGIA LA English DT Review DE Elodea; Invasive species; Fish performance; Freshwater food webs; Management priorities ID FRESH-WATER PLANTS; PIKE ESOX-LUCIUS; H. ST-JOHN; MYRIOPHYLLUM-SPICATUM; BIOLOGICAL INVASIONS; NEW-ZEALAND; CANADENSIS MICHX; SUBMERGED MACROPHYTES; SPATIAL-PATTERN; AQUATIC PLANTS AB Invasive species introductions in Arctic and Subarctic ecosystems are growing as climate change manifests and human activity increases in high latitudes. The aquatic plants of the genus Elodea are potential invaders to Arctic and Subarctic ecosystems circumpolar and at least one species is already established in Alaska, USA. To illustrate the problems of preventing, eradicating, containing, and mitigating aquatic, invasive plants in Arctic and Subarctic ecosystems, we review the invasion dynamics of Elodea and provide recommendations for research and management efforts in Alaska. Foremost, we conclude the remoteness of Arctic and Subarctic systems such as Alaska is no longer a protective attribute against invasions, as transportation pathways now reach throughout these regions. Rather, high costs of operating in remote Arctic and Subarctic systems hinders detection of infestations and limits eradication or mitigation, emphasizing management priorities of prevention and containment of aquatic plant invaders in Alaska and other Arctic and Subarctic systems. C1 [Carey, Michael P.] US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. [Sethi, Suresh A.; Rich, Cecil F.] US Fish & Wildlife Serv, Fisheries & Ecol Serv Div, 1011 East Tudor Rd, Anchorage, AK 99503 USA. [Sethi, Suresh A.; Larsen, Sabrina J.] Alaska Pacific Univ, Fisheries Aquat Sci & Technol Lab, Anchorage, AK 99508 USA. [Sethi, Suresh A.] Cornell Univ, US Geol Survey, New York Cooperat Fish & Wildlife Res Unit, Ithaca, NY 14853 USA. [Larsen, Sabrina J.] Alaska Dept Fish & Game, Div Commercial Fisheries, 1255 W 8th St, Juneau, AK 99811 USA. [Rich, Cecil F.] US Forest Serv, Santa Fe Natl Forest, 11 Forest Ln, Santa Fe, NM 87508 USA. RP Carey, MP (reprint author), US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr, Anchorage, AK 99508 USA. EM mcarey@usgs.gov NR 114 TC 0 Z9 0 U1 11 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0018-8158 EI 1573-5117 J9 HYDROBIOLOGIA JI Hydrobiologia PD SEP PY 2016 VL 777 IS 1 BP 1 EP 19 DI 10.1007/s10750-016-2767-x PG 19 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DQ2GG UT WOS:000379019900001 ER PT J AU Babcock, C Finley, AO Cook, BD Weiskittel, A Woodall, CW AF Babcock, Chad Finley, Andrew O. Cook, Bruce D. Weiskittel, Aaron Woodall, Christopher W. TI Modeling forest biomass and growth: Coupling long-term inventory and LiDAR data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE LiDAR; Forest biomass; Biomass growth; Temporal misalignment; Long-term forest inventory; Bayesian hierarchical models; Markov Chain Monte Carlo; Gaussian process; Geospatial ID ABOVEGROUND BIOMASS; CANOPY HEIGHT; CARBON; AIRBORNE; REGRESSION; VARIABLES; COMPLEX; MISSION; PLOTS AB Combining spatially-explicit long-term forest inventory and remotely sensed information from Light Detection and Ranging (LiDAR) datasets through statistical models can be a powerful tool for predicting and mapping above-ground biomass (AGB) at a range of geographic scales. We present and examine a novel modeling approach to improve prediction of AGB and estimate AGB growth using LiDAR data. The proposed model accommodates temporal misalignment between field measurements and remotely sensed data a problem pervasive in such settings by including multiple time-indexed measurements at plot locations to estimate AGB growth. We pursue a Bayesian modeling framework that allows for appropriately complex parameter associations and uncertainty propagation through to prediction. Specifically, we identify a space-varying coefficients model to predict and map AGB and its associated growth simultaneously. The proposed model is assessed using LiDAR data acquired from NASA Goddard's LiDAR, Hyper-spectral & Thermal imager and field inventory data from the Penobscot Experimental Forest in Bradley, Maine. The proposed model outperformed the time-invariant counterpart models in predictive performance as indicated by a substantial reduction in root mean squared error. The proposed model adequately accounts for temporal misalignment through the estimation of forest AGB growth and accommodates residual spatial dependence. Results from this analysis suggest that future AGB models informed using remotely sensed data, such as LiDAR, may be improved by adapting traditional modeling frameworks to account for temporal misalignment and spatial dependence using random effects. (C) 2016 Elsevier Inc. All rights reserved. C1 [Babcock, Chad] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Finley, Andrew O.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Cook, Bruce D.] NASA, Goddard Space Flight Ctr, Biospher Sci Branch, Code 618, Greenbelt, MD 20742 USA. [Weiskittel, Aaron] Univ Maine, Sch Forest Resources, Orono, ME 04469 USA. [Woodall, Christopher W.] US Forest Serv, USDA, No Res Stn, Forest Inventory & Anal Program, 1992 Folwell Ave, St Paul, MN 55114 USA. RP Babcock, C (reprint author), Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. OI Babcock, Chad/0000-0001-9597-4462 FU U.S. Forest Service [USFS 15-JV-11242307-116]; National Science Foundation (NSF) [DMS-1513481, EF-1137309, EF-1241874, EF-1253225]; NASA Carbon Monitoring System grants FX Data for this study were provided by a unit of the Northern Research Station, U.S. Forest Service, located at the Penobscot Experimental Forest in Maine. Significant funding for collection of these data was provided by the U.S. Forest Service (USFS 15-JV-11242307-116). Andrew Finley was supported by National Science Foundation (NSF) DMS-1513481, EF-1137309, EF-1241874, and EF-1253225, as well as NASA Carbon Monitoring System grants. NR 52 TC 0 Z9 0 U1 29 U2 84 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD SEP 1 PY 2016 VL 182 BP 1 EP 12 DI 10.1016/j.rse.2016.04.014 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DQ3HP UT WOS:000379093700001 ER PT J AU Su, CH Zhang, J Gruber, A Parinussa, R Ryu, D Crow, WT Wagner, W AF Su, Chun-Hsu Zhang, Jing Gruber, Alexander Parinussa, Robert Ryu, Dongryeol Crow, Wade T. Wagner, Wolfgang TI Error decomposition of nine passive and active microwave satellite soil moisture data sets over Australia SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Soil moisture; Microwave remote sensing; Error analysis; SMMR; SSM/I; TMI; AMSR-E; WindSat; AMSR2; SMOS; ERS; ASCAT ID LAND-SURFACE MODEL; TRIPLE COLLOCATION ANALYSIS; PARAMETER RETRIEVAL MODEL; AMSR-E; SOUTHEAST AUSTRALIA; GLOBAL-SCALE; TIME-SERIES; SMOS; IMPACT; PRODUCTS AB Soil moisture is one of the essential climate variables for the Global Climate Observing System (GCOS) that has been prioritized by the ESA's Climate Change Initiative to construct its homogeneous long-term climate record. This requires a consistent characterization of the error structures in the individual data sets, which vary due to changes in instrument configuration and calibration, and retrieval algorithm design. In this paper, the random error and systematic differences in nine passive and active microwave satellite soil moisture products over Australia (time coverage: 1978-present) are estimated in a same manner for SM components at subseasonal and seasonal-to-interannual timescales separately. The multi-scale error structures are found to be non-trivial and vary between the products, giving cause for conducting multi-scale merging with awareness of these differences. Noticeable similarities between the error structures of the satellite products derived from same retrieval algorithm and same measuring frequency however suggest transferability of error parameters between them. Using partial rank correlation analysis, the error maps are linked to statistics on vegetation index, digital elevation, soil moisture and soil temperature, and land cover fractions and mixing in order to explain the observed variability and the similarities between the products. (C) 2016 Elsevier Inc. All rights reserved. C1 [Su, Chun-Hsu; Zhang, Jing; Ryu, Dongryeol] Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic, Australia. [Gruber, Alexander; Wagner, Wolfgang] Vienna Univ Technol, Dept Geodesy & Geoinformat, Vienna, Austria. [Parinussa, Robert] Univ New S Wales, Sch Civil & Environm Engn, Sydney, NSW, Australia. [Crow, Wade T.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Su, CH (reprint author), Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic, Australia. EM csu@unimelb.edu.au OI Su, Chun-Hsu/0000-0003-2504-0466; Wagner, Wolfgang/0000-0001-7704-6857 FU University of Melbourne's Early Career Researcher Grant Scheme FX This research was supported under The University of Melbourne's Early Career Researcher Grant Scheme. We thank our colleagues at Vienna University of Technology (TU-Wien) involved in the pre-processing of the LPRM and active satellite SM products. The LPRM products were produced by Richard de Jeu and colleagues at Vrije University Amsterdam and NASA. The SMOS level 3 data were produced by Centre Aval de Traitement des Donnees SMOS (CATDS), operated for the Centre National d'Etudes Spatiales (CNES, France) by IFREMER (Brest, France). The active data sets were produced by TU-Wien within the framework of EUMETSAT's Satellite Application Facility on Support of Operational Hydrology and Water Management. The MERRA-L data set was produced by NASA Global Modeling and Assimilation Office (GMAO) and made available via Goddard Earth Sciences Data and Information Services Center (GES DISC). NR 80 TC 2 Z9 2 U1 16 U2 24 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD SEP 1 PY 2016 VL 182 BP 128 EP 140 DI 10.1016/j.rse.2016.05.008 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DQ3HP UT WOS:000379093700011 ER PT J AU Bai, KX Chang, NB Yu, HJ Gao, W AF Bai, Kaixu Chang, Ni-Bin Yu, Huijia Gao, Wei TI Statistical bias correction for creating coherent total ozone record from OMI and OMPS observations SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Total column ozone; Bias correction; Data merging; Remote sensing ID REGIONAL CLIMATE MODEL; PROFILER SUITE OMPS; MONITORING INSTRUMENT; CHANGE IMPACT; PRECIPITATION; SIMULATIONS; HOLE; VALIDATION; ALGORITHM; DEPLETION AB A long-term coherent total column ozone (TCO) record is essential to ozone layer variability assessment, especially the detection of early signs of ozone recovery after years of depletion. Because of differences in satellite platforms and instruments design, calibration, and retrieval algorithms, however, significant cross-mission biases are observed between multiple sensor TCO observations in the common time-space domain. To attain a coherent TCO record, observed cross-mission biases should be accurately addressed prior to the data-merging scheme. In this study, a modified statistical bias correction method was proposed based on the quantile-quantile adjustment to remove apparent cross-mission TCO biases between the Ozone Monitoring Instrument (OMI) and Ozone Mapping and Profiler Suite (OMPS). To evaluate the effectiveness of this modified algorithm, the overall inconsistency (OI), a unique time-series similarity measure, was proposed to quantify the improvements of consistency (or similarity) between cross-mission TCO time series data before and after bias correction. Common observations during the overlapped time period of 2012-2015 were used to characterize the systematic bias between OMPS and OMI through the modified bias correction method. TCO observations from OMI during 2004-2015 were then projected to the OMPS level by removing associated cross-mission biases. This modified bias correction scheme significantly improved the overall consistency, with an average improvement of 90% during the overlapped time period at the global scale. In addition to the evaluation of consistency improvements before and after bias correction, impacts of cross-mission biases on long-term trend estimations were also investigated. Comparisons of derived trends from the merged TCO time series before and after bias correction across 38 ground-based stations indicate that cross-mission biases not only affect magnitudes of estimated trends, but also result in different phases of trends. Further comparisons of estimated seasonal TCO trends before and after bias correction at the global scale suggest that trends derived from the bias-corrected time series are more accurate than those without bias correction. Overall, the bias correction scheme developed in this study is essential for preparing an accurate long-term TCO record representative of trend analysis to support future assessment of ozone recovery at the global scale. (C) 2016 Elsevier Inc. All rights reserved. C1 [Bai, Kaixu; Gao, Wei] E China Normal Univ, Key Lab Geog Informat Sci, Minist Educ, Shanghai 200241, Peoples R China. [Bai, Kaixu; Chang, Ni-Bin] Univ Cent Florida, Dept Civil Environm & Construct Engn, Orlando, FL 32816 USA. [Yu, Huijia] Duke Univ, Trinity Coll Arts & Sci, Durham, NC 27708 USA. [Gao, Wei] Colorado State Univ, USDA UV B Monitoring & Res Program, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Gao, Wei] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. RP Chang, NB (reprint author), Univ Cent Florida, Dept Civil Environm & Construct Engn, Orlando, FL 32816 USA. FU Science and Technology Commission of Shanghai Municipality [15dz1207805, 13231203804]; Shanghai Municipal Commission of Health and Family Planning [15GWZK0201]; USDA NIFA [2015-34263-24070] FX The authors would like to acknowledge the NASA'S OMPS and OMI science team for their work producing the total column ozone data used in this study. We also thank those scientists preparing the ground-based total column ozone data that are publically available from the World Ozone and UV Data Center (WOUDC). This work was supported by the Science and Technology Commission of Shanghai Municipality (15dz1207805 and 13231203804), Shanghai Municipal Commission of Health and Family Planning (15GWZK0201) and USDA NIFA (2015-34263-24070). NR 68 TC 0 Z9 0 U1 14 U2 20 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD SEP 1 PY 2016 VL 182 BP 150 EP 168 DI 10.1016/j.rse.2016.05.007 PG 19 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA DQ3HP UT WOS:000379093700013 ER PT J AU Pawlowski, ML Bowen, CR Hill, CB Hartman, GL AF Pawlowski, Michelle L. Bowen, Charles R. Hill, Curtis B. Hartman, Glen L. TI Responses of soybean genotypes to pathogen infection after the application of elicitors SO CROP PROTECTION LA English DT Article DE Anthracnose; Cercospora stem blight; Charcoal rot; Green stem disorder; Plant elicitors; Phytophthora root and stem rot; Pod and stem blight; Sudden death syndrome; Integrated management ID SYSTEMIC ACQUIRED-RESISTANCE; ACIBENZOLAR-S-METHYL; PHENYLALANINE AMMONIA-LYASE; SCLEROTINIA-SCLEROTIORUM; PHYTOPHTHORA-SOJAE; GENE-EXPRESSION; MACROPHOMINA-PHASEOLINA; CYST-NEMATODE; PLANT DEFENSE; CHARCOAL ROT AB Soybean diseases and pests can affect soybean production. One emerging pest management method is to treat plants with chemical elicitors at nontoxic levels to induce host resistance. The objective of this research was to determine if elicitors, benzothiadiazole (BTH), chitosan (CHT), phenylalanine (PHE), and salicylic acid (SA), applied to soybean foliage could alter the response of soybean genotypes to soybean pathogens. Two of the soybean genotypes had been previously shown to produce high or low amounts of reactive oxygen species (ROS) in response to elicitation. In the greenhouse, soybean genotypes were challenged with three pathogens 48 h after elicitation. Plants of the cultivar Pharaoh (susceptible control) treated with SA, and then inoculated with Macrophomina phaseolina had a shorter (alpha = 0.05) stem lesion length (34 mm) than the water control (55 mm). Plants of soybean genotype LD00-2817p (high capacity to produce ROS) and the cultivar Sloan treated with BTH, PHE, or SA, and then inoculated with Phytophthora sojae had greater (alpha = 0.05) survival rates than plants treated with the water control. The four elicitors and a water control were evaluated on LD00-2817p and LDX01-1-65 in the field for two consecutive years. Foliar disease incidence and severity were low for both years, although there were some differences in stem disease ratings. For example, charcoal rot stem severity rating was reduced (alpha = 0.05) from 2.0 in the water control to 1.1 with a PHE treatment for LD00-2817p and was reduced (alpha = 0.05) from 3.8 in the water control to 2.6 with SA for LDX01-1-65 in 2013. Both greenhouse controlled experiments and field experiments showed that genotype-specific elicitation reduced disease severity in some cases, but the differences were greater under controlled-inoculated conditions. Published by Elsevier Ltd. C1 [Pawlowski, Michelle L.; Hill, Curtis B.; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Bowen, Charles R.; Hartman, Glen L.] ARS, USDA, Urbana, IL 61801 USA. [Hill, Curtis B.] Agricen Sci LLC, Pilot Point, TX 76258 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM ghartman@illinois.edu FU United Soybean Board FX This work was financially supported in part by the United Soybean Board. We thank Christopher Hahne, Chelsea Harbach, Jennifer Mleczko, Joseph Schoultes, Jessica Stewart, and Ronald Warsaw for help in establishing the field plots and greenhouse tests. NR 40 TC 0 Z9 0 U1 16 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD SEP PY 2016 VL 87 BP 78 EP 84 DI 10.1016/j.cropro.2016.04.022 PG 7 WC Agronomy SC Agriculture GA DP4FH UT WOS:000378451200013 ER PT J AU Aizawa, K Liu, C Tang, SY Veeramachaneni, S Hu, KQ Smith, DE Wang, XD AF Aizawa, Koichi Liu, Chun Tang, Sanyuan Veeramachaneni, Sudipta Hu, Kang-Quan Smith, Donald E. Wang, Xiang-Dong TI Tobacco carcinogen induces both lung cancer and non-alcoholic steatohepatitis and hepatocellular carcinomas in ferrets which can be attenuated by lycopene supplementation SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article DE ferret; tobacco carcinogen; liver cancer; steatohepatitis; lycopene ID HIGH-FAT DIET; NICOTINIC ACETYLCHOLINE-RECEPTORS; BETA-CAROTENE; APO-10'-LYCOPENOIC ACID; DIFFERENTIAL MECHANISMS; SQUAMOUS METAPLASIA; CELL-PROLIFERATION; HEPATIC STEATOSIS; SK-HEP-1 CELLS; F344 RATS AB Early epidemiologic studies have reported that tobacco smoking, which is causally associated with liver cancer, is an independent risk factor for non-alcoholic fatty liver diseases (NAFLD). Lycopene from tomatoes has been shown to be a potential preventive agent against NAFLD and hepatocellular carcinoma (HCC). In the present study, we investigated whether the tobacco carcinogen 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induces lesions in both lungs and livers of ferrets with or without lycopene intervention. Male ferrets (6 groups, n=8-10) were treated either with NNK (50 mg/kg BW, i.p., once a month for four consecutive months) or saline with or without dietary lycopene supplementation (2.2 and 6.6 mg/kg BW/day, respectively) for 26 weeks. Results demonstrate that NNK exposure results in higher incidences of lung tumors, HCC and steatohepatitis (which is characterized by severe inflammatory cell infiltration with concurrent fat accumulation in liver, hepatocellular ballooning degeneration and increased NF-kappa B expression), as well as elevations in bilirubin and AST levels in ferrets. Lycopene supplementation at two doses prevented NNK-induced expressions of alpha 7 nicotinic acetylcholine receptor in the lung and NF-kappa B and CYP2E1 in the liver and attenuated the NNK-induced mortality and pathological lesions in both the lungs and livers of ferrets. The present study provided strong experimental evidence that the tobacco carcinogen NNK can induce both HCC and steatohepatitis in the ferrets and can be a useful model for studying tobacco carcinogen-associated NAFLD and liver cancer. Furthermore, lycopene could provide potential benefits against smoke carcinogen-induced pulmonary and hepatic injury. C1 [Aizawa, Koichi; Liu, Chun; Tang, Sanyuan; Veeramachaneni, Sudipta; Hu, Kang-Quan; Wang, Xiang-Dong] Tufts Univ, Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Jean Mayer United States Dept Agr, 711 Washington St, Boston, MA 02111 USA. [Aizawa, Koichi] Kagome Co Ltd, Div Res & Dev, Nat & Wellness Res Dept, Tokyo, Tochigi, Japan. [Smith, Donald E.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer United States Dept Agr, Comparat Biol Unit, 711 Washington St, Boston, MA 02111 USA. RP Wang, XD (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Jean Mayer United States Dept Agr, 711 Washington St, Boston, MA 02111 USA. EM xiang-dong.wang@tufts.edu FU NIH/NCI [CA176256]; US Department of Agriculture [1950-51000-064S, 2015-67017] FX Grant sponsor: NIH/NCI; Grant number: CA176256; Grant sponsor: US Department of Agriculture; Grant numbers: 1950-51000-064S (ARS) and 2015-67017(NIFA) NR 53 TC 1 Z9 1 U1 21 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0020-7136 EI 1097-0215 J9 INT J CANCER JI Int. J. Cancer PD SEP 1 PY 2016 VL 139 IS 5 BP 1171 EP 1181 DI 10.1002/ijc.30161 PG 11 WC Oncology SC Oncology GA DP3TR UT WOS:000378418800022 PM 27116542 ER PT J AU Oh, M Kim, EK Jeon, BT Tang, Y Kim, MS Seong, HJ Moon, SH AF Oh, Mirae Kim, Eun-Kyung Jeon, Byong-Tae Tang, Yujiao Kim, Moon S. Seong, Hye-Jin Moon, Sang-Ho TI Chemical compositions, free amino acid contents and antioxidant activities of Hanwoo (Bos taurus coreanae) beef by cut SO MEAT SCIENCE LA English DT Article DE Hanwoo beef; Chemical composition; Free amino acid; Antioxidant activity; QUENCHER procedure ID KOREAN HANWOO; CAPACITY; QUALITY; PROTEINS; STEER; FOODS; ASSAY; MEAT AB The objective of this study was to evaluate chemical compositions, free amino acid contents, and antioxidant activities of different cuts of Hanwoo (Bos taurus coreanae) beef. Beef preferences and prices in the Korean market depend on cut. Therefore, comparisons were made between high-preference cuts (group 1 [G1], including loin, tenderloin, and rib) and low-preference cuts (group 2 [G2], including brisket, topside, and shank). Meat samples were collected from 10 fattened cows. Crude fat content was significantly higher in G1 than in G2 (p < 0.05). The amounts of crude protein and total free amino acid were negatively correlated with crude fat content (p < 0.05). Overall G2 contained higher levels of free amino acids with antioxidant activity than G1. Antioxidant activities were also significantly higher in G2 compared with G1 (p<0.05). In conclusion, providing consumers with positive information about G2 as found in this study could help health-conscious consumers choosing among beef products and further promote increased consumption of low-preference beef cuts. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Oh, Mirae; Kim, Eun-Kyung; Jeon, Byong-Tae; Tang, Yujiao; Seong, Hye-Jin; Moon, Sang-Ho] Konkuk Univ, Div Food Bio Sci, Chungju 27478, South Korea. [Kim, Moon S.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. RP Moon, SH (reprint author), Konkuk Univ, Div Food Bio Sci, Chungju 27478, South Korea. EM moon0204@kku.ac.kr OI Kim, Eun-Kyung/0000-0002-4832-6427 FU Konkuk University FX This paper was supported by Konkuk University in 2016. NR 42 TC 1 Z9 1 U1 3 U2 19 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1740 EI 1873-4138 J9 MEAT SCI JI Meat Sci. PD SEP PY 2016 VL 119 BP 16 EP 21 DI 10.1016/j.meatsci.2016.04.016 PG 6 WC Food Science & Technology SC Food Science & Technology GA DP4FQ UT WOS:000378452100004 PM 27115864 ER PT J AU Cho, KH Pachepsky, YA Oliver, DM Muirhead, RW Park, Y Quilliam, RS Shelton, DR AF Cho, Kyung Hwa Pachepsky, Yakov A. Oliver, David M. Muirhead, Richard W. Park, Yongeun Quilliam, Richard S. Shelton, Daniel R. TI Modeling fate and transport of fecally-derived microorganisms at the watershed scale: State of the science and future opportunities SO WATER RESEARCH LA English DT Review DE Fecal indicator organism (FIO); Catchment scale; Fate and transport model; Non-point source pollution; Pathogens ID ESCHERICHIA-COLI O157-H7; E. COLI; STREAMBED SEDIMENT; INDICATOR BACTERIA; SURFACE WATERS; AGRICULTURAL CATCHMENTS; TEMPERATURE-DEPENDENCE; SALMONELLA-ENTERICA; MANURE CONSTITUENTS; SIMULATED RAINFALL AB Natural waters serve as habitat for a wide range of microorganisms, a proportion of which may be derived from fecal material. A number of watershed models have been developed to understand and predict the fate and transport of fecal microorganisms within complex watersheds, as well as to determine whether microbial water quality standards can be satisfied under site-specific meteorological and/or management conditions. The aim of this review is to highlight and critically evaluate developments in the modeling of microbial water quality of surface waters over the last 10 years and to discuss the future of model development and application at the watershed scale, with a particular focus on fecal indicator organisms (FIOs). In doing so, an agenda of research opportunities is identified to help deliver improvements in the modeling of microbial water quality draining through complex landscape systems. This comprehensive review therefore provides a timely steer to help strengthen future modeling capability of FIOs in surface water environments and provides a useful resource to complement the development of risk management strategies to reduce microbial impairment of freshwater sources. Published by Elsevier Ltd. This is an open access article under the CC BY license C1 [Cho, Kyung Hwa] Ulsan Natl Inst Sci & Technol, Sch Urban & Environm Engn, Ulsan 689798, South Korea. [Pachepsky, Yakov A.; Park, Yongeun; Shelton, Daniel R.] USDA ARS, Environm Microbial & Food Safety Lab, BARC EAST, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. [Oliver, David M.; Quilliam, Richard S.] Univ Stirling, Biol & Environm Sci, Stirling FK9 4LA, Scotland. [Muirhead, Richard W.] AgResearch Ltd, Invermay Res Ctr, Land & Environm, Private Bag 50034, Mosgiel 9053, New Zealand. RP Pachepsky, YA (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, BARC EAST, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. OI Quilliam, Richard/0000-0001-7020-4410; Oliver, David/0000-0002-6200-562X; Park, Yongeun/0000-0002-1959-0843; Muirhead, Richard/0000-0002-0913-561X FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2014R1A1A2059680]; UK Natural Environment Research Council (NERC) as part of the 'PRACTICAL Modelling' project [NE/M005860/1]; USDA-ARS project "Pathogenic microorganisms in irrigation waters"; Clean Water, Productive Land research programme - New Zealand Ministry for Business, Innovation and Employment [C10x1006] FX This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1A1A2059680). DMO & RSQ received funding from the UK Natural Environment Research Council (NERC) as part of the 'PRACTICAL Modelling' project (NE/M005860/1). YP, YP, and DRS were funded via the USDA-ARS project "Pathogenic microorganisms in irrigation waters". RWM was supported by the Clean Water, Productive Land research programme (C10x1006) funded by the New Zealand Ministry for Business, Innovation and Employment. NR 135 TC 2 Z9 2 U1 26 U2 49 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0043-1354 J9 WATER RES JI Water Res. PD SEP 1 PY 2016 VL 100 BP 38 EP 56 DI 10.1016/j.watres.2016.04.064 PG 19 WC Engineering, Environmental; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA DP4EK UT WOS:000378448800005 PM 27176652 ER PT J AU Shedekar, VS King, KW Fausey, NR Soboyejo, ABO Harmel, RD Brown, LC AF Shedekar, Vinayak S. King, Kevin W. Fausey, Norman R. Soboyejo, Alfred B. O. Harmel, R. Daren Brown, Larry C. TI Assessment of measurement errors and dynamic calibration methods for three different tipping bucket rain gauges SO ATMOSPHERIC RESEARCH LA English DT Article DE Precipitation; Tipping bucket rain gauge; Calibration; Accuracy; Rainfall measurement; Hydrologic monitoring ID SAMPLING ERRORS; ACCURACY; PRECIPITATION; SIMULATIONS; DRAINAGE; IMPACT; GAGES; RADAR; TEXAS; WATER AB Three different models of tipping bucket rain gauges (TBRs), viz. HS-TB3 (Hydrological Services Pty Ltd.), ISCO-674 (Isco, Inc.) and TR-525 (Texas Electronics, Inc.), were calibrated in the lab to quantify measurement errors across a range of rainfall intensities (5 mm.h(-1) to 250 mm.h(-1)) and three different volumetric settings. Instantaneous and cumulative values of simulated rainfall were recorded at 1, 2, 5,10 and 20-min intervals. All three TBR models showed a substantial deviation (alpha = 0.05) in measurements from actual rainfall depths, with increasing underestimation errors at greater rainfall intensities. Simple linear regression equations were developed for each TBR to correct the TBR readings based on measured intensities (R-2 > 0.98). Additionally, two dynamic calibration techniques, viz. quadratic model (R-2 > 0.7) and T vs. 1/Q model (R-2 = > 0.98), were tested and found to be useful in situations when the volumetric settings of TBRs are unknown. The correction models were successfully applied to correct field-collected rainfall data from respective TBR models. The calibration parameters of correction models were found to be highly sensitive to changes in volumetric calibration of TBRs. Overall, the HS-TB3 model (with a better protected tipping bucket mechanism, and consistent measurement errors across a range of rainfall intensities) was found to be the most reliable and consistent for rainfall measurements, followed by the ISCO-674 (with susceptibility to clogging and relatively smaller measurement errors across a range of rainfall intensities) and the TR-525 (with high susceptibility to clogging and frequent changes in volumetric calibration, and highly intensity-dependent measurement errors). The study demonstrated that corrections based on dynamic and volumetric calibration can only help minimize but not completely eliminate the measurement errors. The findings from this study will be useful for correcting field data from TBRs; and may have major implications to field- and watershed-scale hydrologic studies. (C) 2016 Elsevier B.V. All rights reserved. C1 [Shedekar, Vinayak S.; Soboyejo, Alfred B. O.; Brown, Larry C.] Ohio State Univ, Dept Food Agr & Biol Engn, 590 Woody Hayes Dr, Columbus, OH 43210 USA. [King, Kevin W.; Fausey, Norman R.] USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr, Columbus, OH 43210 USA. [Harmel, R. Daren] USDA ARS, Grassland Soil & Water Res Lab, 808 East Blackland Rd, Temple, TX 76502 USA. RP Shedekar, VS (reprint author), Ohio State Univ, Dept Food Agr & Biol Engn, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM shedekar.1@osu.edu FU Overholt Drainage Education and Research Program, Department of Food, Agricultural and Biological Engineering, The Ohio State University; USDA-ARS Soil Drainage Research Unit at Columbus, OH; USDA-ARS Grassland, Soil and Water Research Laboratory at Temple, TX FX The authors would like to thank the Overholt Drainage Education and Research Program, Department of Food, Agricultural and Biological Engineering, The Ohio State University; the USDA-ARS Soil Drainage Research Unit at Columbus, OH and the USDA-ARS Grassland, Soil and Water Research Laboratory at Temple, TX for funding and facilitating the experiments conducted for this manuscript. We also thank Vilmos Vasvari, Institute of Urban Water Management and Landscape, Graz, Austria; Alan Flint, Research Hydrologist, USGS, Sacramento, CA; Scott Stephens, Meteorologist, NOAA's National Climatic Data Center, Asheville, NC; Claude Lellevre, Enviromet International Inc.; and Laura Cook, Observing Service Division of NWS for their comments and suggestions during the study. The authors would like to recognize the contributions of M. J. Heckel, Justin McBride, and Lindsay Pease in laboratory and field work, and Dr. Asmita Murumkar, IIT Roorkee, India in internal review of the manuscript. We would also like to thank Dr. Rafiq Islam, The Ohio State University South Centers for his valuable guidance with respect to statistical analyses presented in this manuscript. NR 51 TC 1 Z9 2 U1 21 U2 23 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0169-8095 EI 1873-2895 J9 ATMOS RES JI Atmos. Res. PD SEP 1 PY 2016 VL 178 BP 445 EP 458 DI 10.1016/j.atmosres.2016.04.016 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DP2YY UT WOS:000378360700038 ER PT J AU Clark, PE Williams, CJ Pierson, FB Hardegree, SP AF Clark, Patrick E. Williams, C. Jason Pierson, Fredrick B. Hardegree, Stuart P. TI Postfire grazing management effects on mesic sagebrush-steppe vegetation: Spring grazing SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Burning; Cattle; Fire-grazing interactions; Livestock; Prescribed fire; Rangeland improvement ID FIRE-GRAZER INTERACTIONS; SAGE GROUSE CHICKS; ELK WINTER RANGE; TALLGRASS PRAIRIE; PRESCRIBED-FIRE; BLUEBUNCH WHEATGRASS; COMMUNITY STRUCTURE; UNITED-STATES; LONG-TERM; HYDROLOGIC VULNERABILITY AB The influence of fire-grazing interactions on ecological pattern and process has been fairly well studied in some rangeland ecosystems (e.g., tallgrass prairie) but is only poorly understood in others. On sagebrush steppe rangelands of the western US, there has been a long-standing concern that fire followed by grazing can cause substantial mortality in sensitive plant species. Vegetation responses to fire-grazing interactions, however, have never been studied in the higher elevation, more mesic portions of the sagebrush-steppe. We investigated whether graminoid, forb, and litter cover; bare ground; and species density and frequency responses differed among burned areas which were grazed at a very light stocking rate (33 ha AUM(-1)) during spring (May) without postfire deferment, burned areas where 1-2 growing seasons of grazing deferment were applied, and burned areas completely excluded from postfire grazing. Fire-grazing interactions had very few effects on vegetation but did reduce litter cover and bare ground compared to burning alone. This was a case study; consequently, caution should be taken in applying these results beyond their limited scope of inference. In some situations, however, postfire grazing can likely be employed without deferment or after deferring for only one growing season, and not cause substantial adverse impacts on vegetation. Published by Elsevier Ltd. C1 [Clark, Patrick E.; Williams, C. Jason; Pierson, Fredrick B.; Hardegree, Stuart P.] USDA ARS, Northwest Watershed Res Ctr, 800 E Pk Blvd,Suite 105, Boise, ID 83712 USA. RP Clark, PE (reprint author), USDA ARS, Northwest Watershed Res Ctr, 800 E Pk Blvd,Suite 105, Boise, ID 83712 USA. EM pat.clark@ars.usda.gov OI Williams, Jason/0000-0002-6289-4789 FU USDA Agricultural Research Service FX Funding for this research was provided by the USDA Agricultural Research Service. Prescribed burning was expertly conducted by the Boise District, USDI Bureau of Land Management, Boise, ID. NR 83 TC 0 Z9 0 U1 8 U2 31 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 EI 1095-922X J9 J ARID ENVIRON JI J. Arid. Environ. PD SEP PY 2016 VL 132 BP 49 EP 59 DI 10.1016/j.jaridenv.2015.10.022 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DO6XU UT WOS:000377927600006 ER PT J AU Ma, JC Ibekwe, AM Yang, CH Crowley, DE AF Ma, Jincai Ibekwe, A. Mark Yang, Ching-Hong Crowley, David E. TI Bacterial diversity and composition in major fresh produce growing soils affected by physiochemical properties and geographic locations SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Bacterial ecology; Pyrosequencing; Artificial neural network ID ESCHERICHIA-COLI O157H7; MICROBIAL COMMUNITIES; LAND-USE; CONVENTIONAL MANAGEMENT; AGRICULTURAL SOILS; SALINITY GRADIENT; TIBETAN PLATEAU; ORGANIC-MATTER; BIOGEOGRAPHY; PATTERNS AB Microbial diversity of agricultural soils has been well documented, but information on leafy green producing soils is limited. In this study, we investigated microbial diversity and community structures in 32 (16 organic, 16 conventionally managed soils) from California (CA) and Arizona (AZ) using pyrosequencing, and identified factors affecting bacterial composition. Results of detrended correspondence analysis (DCA) and dissimilarity analysis showed that bacterial community structures of conventionally managed soils were similar to that of organically managed soils; while the bacterial community structures in soils from Salinas, California were different (P < 0.05) from those in soils from Yuma, Arizona and Imperial Valley, California. Canonical correspondence analysis (CCA) and artificial neural network (ANN) analysis of bacterial community structures and soil variables showed that electrical conductivity (EC), clay content, water-holding capacity (WHC), pH, total nitrogen (TN), and organic carbon (OC) significantly (P < 0.05) correlated with microbial communities. CCA based variation partitioning analysis (VPA) showed that soil physical properties (clay, EC, and WHC), soil chemical variables (pH, TN, and OC) and sampling location explained 16.3%, 12.5%, and 50.9%, respectively, of total variations in bacterial community structure, leaving 13% of the total variation unexplained. Our current study showed that bacterial community composition and diversity in major fresh produce growing soils from California and Arizona is a function of soil physiochemical characteristics and geographic distances of sampling sites. Published by Elsevier B.V. C1 [Ma, Jincai] Jilin Univ, Minist Educ, Key Lab Groundwater Resources & Environm, Changchun 130021, Peoples R China. [Ma, Jincai; Ibekwe, A. Mark] ARS, USDA, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. [Yang, Ching-Hong] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA. [Crowley, David E.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. RP Ibekwe, AM (reprint author), ARS, USDA, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Mark.Ibekwe@ars.usda.gov RI Ma, Jincai/D-1290-2013 OI Ma, Jincai/0000-0002-0792-0251 FU CSREES NIFA [2008-35201-18709]; National Natural Science Foundation of China [41571304] FX This research was supported by CSREES NIFA Agreement (No. 2008-35201-18709) and the 206 Manure and Byproduct Utilization Project of the USDA-ARS. The research was also partly financed by National Natural Science Foundation of China (No. 41571304). We thank Drs Jorge Fonseca of the University of Arizona Yuma, Mark Trent, UC-Davis, Imperial Agricultural Experiment Station, and James McCreight of USDA-ARS Salinas CA for providing soil samples for this study. We also thank Damon Baptista for technical help. Mention of trademark or propriety products in this manuscript does not constitute a guarantee or warranty of the property by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 64 TC 1 Z9 1 U1 15 U2 50 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD SEP 1 PY 2016 VL 563 BP 199 EP 209 DI 10.1016/j.scitotenv.2016.04.122 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA DO4ZG UT WOS:000377792800023 PM 27135583 ER PT J AU Ulyshen, MD Muller, J Seibold, S AF Ulyshen, Michael D. Mueller, Joerg Seibold, Sebastian TI Bark coverage and insects influence wood decomposition: Direct and indirect effects SO APPLIED SOIL ECOLOGY LA English DT Article DE Blattodea; Ecosystem services; Isoptera; Saproxylic ID PENINSULAR MALAYSIA; LIGNIN DEGRADATION; TERMITE EXCLUSION; DEAD-WOOD; FUNGI; DECAY; FORESTS; TRAITS AB Rates of terrestrial wood decomposition are known to vary widely depending on regional and local climatic conditions, substrate characteristics and the organisms involved but the influence of many factors remain poorly quantified. We sought to determine how bark and insects contribute to decomposition in a southeastern U.S. forest. Open-topped stainless steel pans with screened bottoms were used to prevent subterranean termite (Rhinotermitidae: Reticulitermes spp.) colonization from "protected" logs. After a 20-month study period, we compared mass loss and lignin content between these and logs assigned to "unprotected" treatments that permitted termite colonization. The experiment was repeated for 1) logs from which bark had or had not been initially removed and 2) logs with sealed or unsealed ends. Logs with bark lost significantly (similar to 2.4-fold) more mass than those without bark, likely due to the moisture-conserving properties of bark. Logs with unsealed ends lost significantly more mass than those with sealed ends. There was no significant difference in mass loss between protected and unprotected logs but logs with visible termite activity lost significantly more mass than those without termite damage. Few differences in lignin content were detected in this study but when logs with bark were analyzed separately, those with visible damage from long-horned beetles (Coleoptera: Cerambycidae) had lower lignin content than those without cerambycid activity. This suggests that cerambycids may promote decomposition indirectly through interactions with fungi or other organisms capable of degrading lignin. Our results suggest that insects can have significant direct and indirect effects on wood decomposition and clearly demonstrate the importance of bark in determining wood decay rates and insect activity. Published by Elsevier B.V. C1 [Ulyshen, Michael D.] US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. [Mueller, Joerg; Seibold, Sebastian] Bavarian Forest Natl Pk, Freyunger Str 2, D-94481 Grafenau, Germany. [Mueller, Joerg; Seibold, Sebastian] Tech Univ Munich, Terr Ecol Res Grp, Dept Ecol & Ecosyst Management, Ctr Food & Life Sci Weihenstephan, Hans Carl von Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. EM mulyshen@fs.fed.us FU USDA Forest Service, Southern Research Station FX This research was supported by the USDA Forest Service, Southern Research Station. NR 27 TC 2 Z9 2 U1 22 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD SEP PY 2016 VL 105 BP 25 EP 30 DI 10.1016/j.apsoil.2016.03.017 PG 6 WC Soil Science SC Agriculture GA DN8VP UT WOS:000377358300004 ER PT J AU Moser, BR Seliskar, DM Gallagher, JL AF Moser, Bryan R. Seliskar, Denise M. Gallagher, John L. TI Fatty acid composition of fourteen seashore mallow (Kosteletzkya pentacarpos) seed oil accessions collected from the Atlantic and Gulf coasts of the United States SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Dihydrosterculic acid; Fatty acid; Kosteletzkya pentacarpos; Malvalic acid; Seashore mallow; Sterculic acid ID RENEWABLE RAW-MATERIALS; BIODIESEL PRODUCTION; VIRGINICA; CROPS; FEEDSTOCKS; TOLERANCE; MALVACEAE; PROFILE; CHINA AB Seashore mallow (Kosteletzkya pentacarpos) is a flowering perennial halophytic herb belonging to the family Malvaceae that is found in marshes along the Atlantic and Gulf coasts of the United States. Fourteen accessions were collected from wild populations along the Atlantic (n = 8) and Gulf (n = 6) coasts of the United States and fatty acid profiles were determined from seeds cultivated in one or more of three controlled environments. Those growth environments were a greenhouse and field plots irrigated with freshwater and field plots irrigated with saltwater, all of which were located in Lewes, DE. Twenty-four of the 42 combinations were selected for fatty acid analysis. The principal fatty acid identified in all accessions was linoleic acid (53.1 +/- 2.8%), with significant amounts of palmitic (20.7 +/- 1.3%), oleic (13.7 +/- 1.4%), and stearic (1.8 +/- 0.5%) acids also detected. Additionally, significant amounts of cyclopropyl fatty acids such as malvalic (5.2 +/- 0.7%) and dihydrosterculic (1.2 +/- 0.5%) acids were also identified, along with trace amounts of sterculic acid. Minor fatty acids (<1.0%) included myristic, palmitoleic, vaccenic, linolenic, arachidic, and behenic acids. Statistical evaluation revealed that growth environment did not affect fatty acid composition, as statistically equivalent profiles were obtained regardless of whether accessions were grown in a greenhouse or in field plots with freshwater or saltwater irrigation. One-way ANOVA revealed statistically significant differences in palmitic, stearic, oleic, and linoleic acid content between Gulf and Atlantic coast accessions, with Gulf coast accessions providing lower mean oleic and palmitic acid content and higher mean linoleic and stearic acid content. In addition, Gulf coast accessions contained lower mean dihydrosterculic and malvalic acid content. Published by Elsevier B.V. C1 [Moser, Bryan R.] ARS, Biooils Res, Unit Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Seliskar, Denise M.; Gallagher, John L.] Univ Delaware, Coll Earth Ocean & Environm, Halophyte Biotechnol Ctr, 700 Pilottown Rd, Lewes, DE 19958 USA. RP Moser, BR (reprint author), ARS, Biooils Res, Unit Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Bryan.Moser@ars.usda.gov NR 29 TC 0 Z9 0 U1 5 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2016 VL 87 BP 20 EP 26 DI 10.1016/j.indcrop.2016.04.018 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA DN8IT UT WOS:000377323100004 ER PT J AU Hinton, A AF Hinton, Arthur, Jr. TI Growth of Campylobacter incubated aerobically in fumarate-pyruvate media or media supplemented with dairy, meat, or soy extracts and peptones SO FOOD MICROBIOLOGY LA English DT Article DE Campylobacter; Media; Aerobic growth; Peptones; Organic acids ID ORGANIC-ACIDS; JEJUNI; OXYGEN AB The ability of Campylobacter to grow aerobically in media supplemented with fumarate-pyruvate or with dairy, meat, or soy extracts or peptones was examined. Optical densities (OD) of Campylobacter cultured in basal media, media supplemented with fumarate-pyruvate or with 1.0, 2.5, 5.0, or 7.5% beef extract was measured. Growth was also compared in media supplemented with other extracts or peptones. Finally, cfu/mL of Campylobacter recovered from basal media or media supplemented with fumarate-pyruvate, casamino acids, beef extract, soytone, or beef extract and soytone was determined. Results indicated that OD of cultures grown in media supplemented with fumarate-pyruvate or with 5.0 or 7.5% beef extract were higher than OD of isolates grown in basal media or media supplemented with lower concentrations of beef extract. Highest OD were produced by isolates grown in media supplemented with beef extract, peptone from meat, polypeptone, proteose peptone, or soytone. Also, more cfu/mL were recovered from media with fumarate-pyruvate, beef extract, soytone, or beef extract-soytone than from basal media or media with casamino acids. Findings indicate that media supplemented with organic acids, vitamins, and minerals and media supplemented with extracts or peptones containing these metabolites can support aerobic growth of Campylobacter. Published by Elsevier Ltd. C1 [Hinton, Arthur, Jr.] US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Unit, ARS, USDA,Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Hinton, A (reprint author), US Natl Poultry Res Ctr, Poultry Microbiol Safety & Proc Unit, ARS, USDA,Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM arthur.hinton@ars.usda.gov NR 21 TC 1 Z9 1 U1 5 U2 9 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD SEP PY 2016 VL 58 BP 23 EP 28 DI 10.1016/j.fm.2016.03.010 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA DM7BZ UT WOS:000376509200005 PM 27217355 ER PT J AU Upadhyay, A Chen, CH Yin, HB Upadhyaya, I Fancher, S Liu, YY Nair, MS Jankelunas, L Patel, JR Venkitanarayanan, K AF Upadhyay, Abhinav Chen, Chi-hung Yin, Hsinbai Upadhyaya, Indu Fancher, Samantha Liu, Yanyan Nair, Meera Surendran Jankelunas, Leanne Patel, Jitendra R. Venkitanarayanan, Kumar TI Inactivation of Listeria monocytogenes, Salmonella spp. and Escherichia coli O157:H7 on cantaloupes by octenidine dihydrochloride SO FOOD MICROBIOLOGY LA English DT Article DE Octenidine hydrochloride; Chitosan; Cantaloupes; Listeria monocytogenes; Escherichia coli O157:H7; Salmonella spp ID FRESH-CUT CANTALOUPE; STAPHYLOCOCCUS-AUREUS; SHELF-LIFE; PATHOGENIC MICROORGANISMS; PSEUDOMONAS-AERUGINOSA; SURFACE PASTEURIZATION; REDUCING SALMONELLA; HYDROGEN-PEROXIDE; SODIUM LACTATE; WHOLE AB The efficacy of a new generation disinfectant, octenidine dihydrochloride (OH), as wash and coating treatments for reducing Listeria monocytogenes (LM), Salmonella spp. (SAL), and Escherichia coli O157:H7 (EC) on cantaloupe was investigated. Cantaloupe rind plugs inoculated separately with the three bacterial species (similar to 8 log CFU/cm(2)) were washed for 1, 3, 5 min at 25 degrees C in water, or chlorine (200 ppm), ethanol (1%), OH (0.01, 0.05, 0.1%) and surviving populations were measured after treatment. Additionally, inoculated cantaloupe rind plugs were coated with 2% chitosan or chitosan containing OH (0.01, 0.05, 0.1%) and sampled for surviving pathogens. Subsequently, the antimicrobial efficacy of OH wash and coating (0.1, 0.2%) on whole cantaloupes was determined. All OH wash reduced LM, SAL, and EC on cantaloupe rinds by > 5 log CFU/cm(2) by 2 min, and reduced populations to undetectable levels (below 2 log CFU/cm(2)) by 5 min (P < 0.05). Similarly, OH coating on cantaloupe rinds reduced the pathogens by 3 -5 log /cm(2) (P < 0.05). Washing and coating whole cantaloupes with OH reduced the three pathogens by at least 5 log and 2 log CFU/cm(2), respectively (P < 0.05). Results suggest that OH could be used as antimicrobial wash and coating to reduce LM, SAL, and EC on cantaloupes. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Upadhyay, Abhinav; Chen, Chi-hung; Yin, Hsinbai; Upadhyaya, Indu; Fancher, Samantha; Liu, Yanyan; Nair, Meera Surendran; Jankelunas, Leanne; Venkitanarayanan, Kumar] Univ Connecticut, Dept Anim Sci, 3636 Horse Barn Hill Rd Ext,Unit 4040, Storrs, CT 06269 USA. [Patel, Jitendra R.] USDA ARS, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave,Bldg 201 BARC East,Room 101, Beltsville, MD 20705 USA. RP Venkitanarayanan, K (reprint author), Univ Connecticut, Dept Anim Sci, 3636 Horse Barn Hill Rd Ext,Unit 4040, Storrs, CT 06269 USA. EM kumar.venkitanarayanan@uconn.edu OI Yin, Hsinbai/0000-0002-1175-4516; Upadhyay, Abhinav/0000-0002-6064-4858; Upadhyaya, Indu/0000-0003-2818-9207 FU USDA-NIFA critical and emerging food safety issues program [2012-04333] FX This study was funded by a grant (2012-04333) awarded to K. Venkitanarayanan from the USDA-NIFA critical and emerging food safety issues program. NR 61 TC 0 Z9 0 U1 7 U2 16 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD SEP PY 2016 VL 58 BP 121 EP 127 DI 10.1016/j.fm.2016.04.007 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA DM7BZ UT WOS:000376509200017 PM 27217367 ER PT J AU Guo, AL Xu, YF Mowery, J Nagy, A Bauchan, G Nou, XW AF Guo, Ailing Xu, Yunfeng Mowery, Joseph Nagy, Attila Bauchan, Gary Nou, Xiangwu TI Ralstonia insidiosa induces cell aggregation of Listeria monocytogenes SO FOOD CONTROL LA English DT Article DE Listeria monocytogenes; Ralstonia insidiosa; Cell aggregation; Biofilms; TEM ID ESCHERICHIA-COLI O157H7; BIOFILM FORMATION; STRAINS; COAGGREGATION; PREVALENCE; CANTALOUPE; PICKETTII; OUTBREAK; GROWTH; LEVEL AB Biofilm formation is an important strategy for foodborne bacterial pathogens to survive in stressful environments such as fresh produce processing facilities. Bacterial cell aggregation strongly promotes the initiation of microcolonies and the formation of biofilms on abiological surfaces. We previously showed that Ralstonia insidiosa, an environmental bacterial species frequently isolated from fresh produce facilities, may serve as a "bridge bacterium" that strongly enhanced the incorporation of several foodborne bacterial pathogens into dual species biofilms. While the R. insidiosa strain exhibited moderate cell aggregation in liquid culture, co-culturing Listeria monocytogenes with R. insidiosa resulted in significant augmentation of cell aggregation. Electron microscopy indicated that L. monocytogenes cells were initially attracted to the R. insidiosa aggregates and formed large dual species aggregates that were predominately composed of L. monocytogenes cells. The predominant presence of L. monocytogenes in the dual species aggregates was also confirmed by differential plating. These findings suggest that bridge bacteria such as R. insidiosa play critical roles in the survival of foodborne bacterial pathogens, such as L. monocytogenes and Escherichia coli, by promoting multispecies biofilm formation. The implications of such bridge bacteria on food safety need to be further evaluated. Published by Elsevier Ltd. C1 [Guo, Ailing] Huazhong Agr Univ, Natl Res & Dev Ctr Egg Proc, Wuhan 430070, Peoples R China. [Xu, Yunfeng; Nagy, Attila; Nou, Xiangwu] ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Mowery, Joseph; Bauchan, Gary] ARS, Electron & Confocal Microscopy Unit, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Nagy, Attila] NIAID, NIH, Vaccine Res Ctr, Gaithersburg, MD 20878 USA. RP Nou, XW (reprint author), ARS, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Xiangwu.nou@ars.usda.gov FU Huazhong Agricultural University; China Scholarship Council FX Authors wish to thank Dr. T. Ward for providing L.monocytogenes strains used in this study. Ailing Guo was a visiting scientist to USDA ARS EMFSL from and supported by Huazhong Agricultural University, and Yunfeng Xu is predoctoral student from Northwest A&F University, China, and is supported by China Scholarship Council. NR 37 TC 0 Z9 0 U1 6 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD SEP PY 2016 VL 67 BP 303 EP 309 DI 10.1016/j.foodcont.2016.03.006 PG 7 WC Food Science & Technology SC Food Science & Technology GA DK8FY UT WOS:000375163800039 ER PT J AU Chagas, BME Dorado, C Serapiglia, MJ Mullen, CA Boateng, AA Melo, MAF Ataide, CH AF Chagas, Bruna M. E. Dorado, Christina Serapiglia, Michelle J. Mullen, Charles A. Boateng, Akwasi A. Melo, Marcus A. F. Ataide, Carlos H. TI Catalytic pyrolysis-GC/MS of Spirulina: Evaluation of a highly proteinaceous biomass source for production of fuels and chemicals SO FUEL LA English DT Article DE Aromatics; Cyanobacteria; Pyrolysis; Microalgae; Spirulina; Zeolites ID BIO-OIL PRODUCTION; LIGNOCELLULOSIC BIOMASS; ZEOLITE CATALYSTS; BED REACTOR; MICROALGAE; COMPONENTS; CONVERSION; QUALITY; HYDROCARBONS; AROMATICS AB The catalytic pyrolysis of Spirulina (Arthrospira spp.) over several zeolite catalysts using pyrolysis/GC-MS was studied to explore the optimum and scalable conditions for the production of stable liquid intermediates via analysis of the condensable vapors. Nine catalysts of different pore sizes, shapes and SiO2/Al2O3 ratios were tested in this study including H-ZSM5 (SiO2/Al2O3 = 23), H-ZSM5 (50), H-ZSM5 (280), H-beta (25), H-beta (38), H-beta (300), H-Y, mordenite and ferrierite at three catalyst/biomass ratios (1:1, 5:1 and 10:1). It was found that the high acidity HZSM-5 catalysts with low SiO2/Al2O3 ratios (Si/Al = 23) could maximize the conversion of Spirulina to aromatic hydrocarbons, but lower acidity catalysts increased the production of aliphatic hydrocarbons, phenols and certain nitrogenates. The component analysis shows that it is possible to favor one set of chemical species over another for the conversion of Spirulina by varying the catalyst types and loadings allowing for the flexibility to engineer scaled systems for greater yields of particular chemical classes such as aromatic hydrocarbons, phenols, and aromatic nitrogenates via the manipulation of the catalyst properties or loadings. Published by Elsevier Ltd. C1 [Chagas, Bruna M. E.; Melo, Marcus A. F.] Univ Fed Rio Grande do Norte, CT, Dept Engn Quim, BR-59078970 Natal, RN, Brazil. [Chagas, Bruna M. E.; Dorado, Christina; Serapiglia, Michelle J.; Mullen, Charles A.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Ataide, Carlos H.] Univ Fed Uberlandia, Fac Engn Quim, Campus Santa Monica,Bloco 1K, BR-38408144 Uberlandia, MG, Brazil. RP Mullen, CA; Boateng, AA (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov; akwasi.boateng@ars.usda.gov FU Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes); USDA-ARS/UFRN NFCA Project [8072-41000-095-24] FX This research was financially supported by Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Capes) and performed under the USDA-ARS/UFRN NFCA Project 8072-41000-095-24. The authors acknowledge Patrick West for his help in carrying out the analytical routines. NR 44 TC 4 Z9 4 U1 22 U2 84 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0016-2361 EI 1873-7153 J9 FUEL JI Fuel PD SEP 1 PY 2016 VL 179 BP 124 EP 134 DI 10.1016/j.fuel.2016.03.076 PG 11 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DK5RJ UT WOS:000374977300016 ER PT J AU O'Neil, GW Knothe, G Williams, JR Burlow, NP Reddy, CM AF O'Neil, Gregory W. Knothe, Gerhard Williams, John R. Burlow, Noah P. Reddy, Christopher M. TI Decolorization improves the fuel properties of algal biodiesel from Isochrysis sp. SO FUEL LA English DT Article DE Biodiesel; Algae; Decolorization; Fuel properties ID ACID METHYL-ESTERS; CARBON-DIOXIDE; CETANE NUMBERS; CANOLA OIL; MICROALGAE; DIESEL; TEMPERATURE; PREDICTION; ALKENONES; PROFILE AB Results from the comprehensive fuel testing according to American Society for Testing and Materials International (ASTM) standards of an alkenone-free and decolorized biodiesel produced from the industrially grown marine microalgae Isochrysis sp. are presented. Fatty acid methyl ester (FAME) profiles of the non-decolorized and subsequently decolorized biodiesel fuels were nearly identical, yet the fuel properties were remarkably different. Significant positive impacts on the cetane number, kinematic viscosity, and lubricity were observed, indicating a potential deleterious effect of pigments like chlorophylls and pheophytins on these fuel properties. The decolorization process using montmorillonite K10 gave on average 90% mass recovery, and allowed for an otherwise unobtainable cloud point determination. Oxidative stability of the decolorized Isochrysis biodiesel remained well below the minimum prescribed in biodiesel standards due to elevated content of highly polyunsaturated fatty acids, however other values were in the range of those prescribed in the ASTM standards. Overall, decolorization improved the fuel properties of biodiesel from Isochrysis and may provide a path toward improved biodiesel fuels from other algal species. (C) 2016 Elsevier Ltd. All rights reserved. C1 [O'Neil, Gregory W.; Williams, John R.; Burlow, Noah P.] Western Washington Univ, Dept Chem, 516 High St, Bellingham, WA 98225 USA. [Knothe, Gerhard] USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. [Reddy, Christopher M.] Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA. RP O'Neil, GW (reprint author), Western Washington Univ, Dept Chem, 516 High St, Bellingham, WA 98225 USA. EM oneil@chem.wwu.edu FU National Science Foundation [CHE-1151492] FX This work was supported by the National Science Foundation (CHE-1151492) and through a private donation from friends of WHOI. We thank Kevin R. Steidley and Kim Ascherl (USDA/ARS/NCAUR) for excellent technical assistance, and Michael Wibbens (Southwest Research Institute, San Antonio, TX) for cetane testing. NR 48 TC 1 Z9 1 U1 7 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0016-2361 EI 1873-7153 J9 FUEL JI Fuel PD SEP 1 PY 2016 VL 179 BP 229 EP 234 DI 10.1016/j.fuel.2016.03.061 PG 6 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DK5RJ UT WOS:000374977300027 ER PT J AU Frost, CM Peralta, G Rand, TA Didham, RK Varsani, A Tylianakis, JM AF Frost, Carol M. Peralta, Guadalupe Rand, Tatyana A. Didham, Raphael K. Varsani, Arvind Tylianakis, Jason M. TI Apparent competition drives community-wide parasitism rates and changes in host abundance across ecosystem boundaries SO NATURE COMMUNICATIONS LA English DT Article ID FOOD WEBS; NATURAL ENEMIES; BIOCONTROL; PREDATORS; NEMATODES; FOREST; BIODIVERSITY; LEPIDOPTERA; DISPERSAL; MUTUALISM AB Species have strong indirect effects on others, and predicting these effects is a central challenge in ecology. Prey species sharing an enemy (predator or parasitoid) can be linked by apparent competition, but it is unknown whether this process is strong enough to be a community-wide structuring mechanism that could be used to predict future states of diverse food webs. Whether species abundances are spatially coupled by enemy movement across different habitats is also untested. Here, using a field experiment, we show that predicted apparent competitive effects between species, mediated via shared parasitoids, can significantly explain future parasitism rates and herbivore abundances. These predictions are successful even across edges between natural and managed forests, following experimental reduction of herbivore densities by aerial spraying of insecticide over 20 hectares. This result shows that trophic indirect effects propagate across networks and habitats in important, predictable ways, with implications for landscape planning, invasion biology and biological control. C1 [Frost, Carol M.; Peralta, Guadalupe; Varsani, Arvind; Tylianakis, Jason M.] Univ Canterbury, Sch Biol Sci, Ctr Integrat Ecol, Private Bag 4800, Christchurch 8140, New Zealand. [Rand, Tatyana A.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Didham, Raphael K.] Univ Western Australia, Sch Anim Biol, 35 Stirling Highway, Crawley, WA 6009, Australia. [Didham, Raphael K.] CSIRO Land & Water, Ctr Environm & Life Sci, Underwood Ave, Floreat, WA 6014, Australia. [Varsani, Arvind] Univ Canterbury, Biomol Interact Ctr, Private Bag 4800, Christchurch 8140, New Zealand. [Varsani, Arvind] Univ Cape Town, Struct Biol Res Unit, Dept Clin Lab Sci, ZA-7700 Cape Town, South Africa. [Varsani, Arvind] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Varsani, Arvind] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Tylianakis, Jason M.] Imperial Coll London, Dept Life Sci, Silwood Pk Campus,Buckhurst Rd, Ascot SL5 7PY, Berks, England. [Frost, Carol M.] Swedish Univ Agr Sci, Dept Forest Ecol & Management, S-90183 Umea, Sweden. [Peralta, Guadalupe] Consejo Nacl Invest Cient & Tecn, Inst Argentino Invest Zonas Aridas, CC 507, RA-5500 Mendoza, Argentina. RP Frost, CM; Tylianakis, JM (reprint author), Univ Canterbury, Sch Biol Sci, Ctr Integrat Ecol, Private Bag 4800, Christchurch 8140, New Zealand.; Tylianakis, JM (reprint author), Imperial Coll London, Dept Life Sci, Silwood Pk Campus,Buckhurst Rd, Ascot SL5 7PY, Berks, England. EM carol7frost@gmail.com; jason.tylianakis@canterbury.ac.nz OI Tylianakis, Jason/0000-0001-7402-5620 FU Natural Sciences and Engineering Research Council of Canada; Education New Zealand; University of Canterbury; Marsden Fund [UOC-0802]; Australian Research Council [FT100100040]; National Research Foundation of South Africa; Rutherford Discovery Fellowship FX We thank Nelson Forests Ltd., Merrill & Ring, Hancock Timber Resource Group, M. Turbitt, D. Bryant, N. Buchanan, L. and P. Douglas, and the Department of Conservation for forest access. J. Dugdale, J. Berry, and R. Schnitzler provided taxonomic assistance. Members of the Ladley family (J., B., D., D., and S.) D. Conder, N. Etheridge, and D. Payton assisted with field and lab logistics. Y. Brindle, C. Hohe, S. Litchwark, S. Hunt, A. McLeod, L. O'Brien, A. Knight, L. Williamson, T. Lambert, H. McFarland, E. Allen, C. Thomas, R. McGee, K. Trotter, T. Watson, V. Nguyen, A. Young, D. Davies, and M. Bartlett assisted with caterpillar collection and rearing. The Tylianakis and Stouffer labs, J. Beggs, H.C.J. Godfray, K.S. McCann, and T. Roslin provided comments on the manuscript. C.M.F. was supported by the Natural Sciences and Engineering Research Council of Canada, Education New Zealand, and the University of Canterbury. The project itself, J.M.T and G.P. were supported by the Marsden Fund (UOC-0802). R.K.D. was funded by an Australian Research Council Future Fellowship FT100100040. A.V. is supported by the National Research Foundation of South Africa. J.M.T. is funded by a Rutherford Discovery Fellowship, administered by the Royal Society of New Zealand. NR 57 TC 0 Z9 0 U1 10 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD AUG 31 PY 2016 VL 7 AR 12644 DI 10.1038/ncomms12644 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA EH6HO UT WOS:000391874000001 PM 27577948 ER PT J AU Rangel, LI Henkels, MD Shaffer, BT Walker, FL Davis, EW Stockwell, VO Bruck, D Taylor, BJ Loper, JE AF Rangel, Lorena I. Henkels, Marcella D. Shaffer, Brenda T. Walker, Francesca L. Davis, Edward W., II Stockwell, Virginia O. Bruck, Denny Taylor, Barbara J. Loper, Joyce E. TI Characterization of Toxin Complex Gene Clusters and Insect Toxicity of Bacteria Representing Four Subgroups of Pseudomonas fluorescens SO PLOS ONE LA English DT Article ID CATERPILLARS-FLOPPY MCF; BIOLOGICAL-CONTROL; PHOTORHABDUS-BACTERIA; PHYLOGENETIC ANALYSIS; COLONIZATION; SPP.; IDENTIFICATION; PHENOLOXIDASE; RHIZOSPHERE; SEQUENCE AB Ten strains representing four lineages of the Pseudomonas fluorescens group (P. chlororaphis, P. corrugata, P. koreensis, and P. fluorescens subgroups) were evaluated for toxicity to the tobacco hornworm Manduca sexta and the common fruit fly Drosophila melanogaster. The three strains within the P. chlororaphis subgroup exhibited both oral and injectable toxicity to the lepidopteran M. sexta. All three strains have the gene cluster encoding the FitD insect toxin and a Delta fitD mutant of P. protegens strain Pf-5 exhibited diminished oral toxicity compared to the wildtype strain. Only one of the three strains, P. protegens Pf-5, exhibited substantial levels of oral toxicity against the dipteran D. melanogaster. Three strains in the P. fluorescens subgroup, which lack fitD, consistently showed significant levels of injectable toxicity against M. sexta. In contrast, the oral toxicity of these strains against D. melanogaster was variable between experiments, with only one strain, Pseudomonas sp. BG33R, causing significant levels of mortality in repeated experiments. Toxin complex (Tc) gene clusters, which encode insecticidal properties in Photorhabdus luminescens, were identified in the genomes of seven of the ten strains evaluated in this study. Within those seven genomes, six types of Tc gene clusters were identified, distinguished by gene content, organization and genomic location, but no correlation was observed between the presence of Tc genes and insect toxicity of the evaluated strains. Our results demonstrate that members of the P. fluorescens group have the capacity to kill insects by both FitD-dependent and independent mechanisms. C1 [Rangel, Lorena I.; Henkels, Marcella D.; Stockwell, Virginia O.; Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Henkels, Marcella D.; Shaffer, Brenda T.; Davis, Edward W., II; Stockwell, Virginia O.; Bruck, Denny; Loper, Joyce E.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97333 USA. [Walker, Francesca L.; Taylor, Barbara J.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Rangel, Lorena I.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Bruck, Denny] DuPont Pioneer, Johnston, IA USA. RP Loper, JE (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Loper, JE (reprint author), ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97333 USA. EM loperj@science.oregonstate.edu OI Loper, Joyce/0000-0003-3501-5969 FU Oregon State University; United States Department of Agriculture National Institute of Food and Agriculture [2008-35600-18770, 2011-67019-30192] FX LIR was supported by a Provost's Distinguished Graduate Fellowship from Oregon State University. This work was supported by Agriculture and Food Research Initiative Competitive Grant 2008-35600-18770 and 2011-67019-30192 from the United States Department of Agriculture National Institute of Food and Agriculture. NR 64 TC 0 Z9 0 U1 4 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 31 PY 2016 VL 11 IS 8 AR e0161120 DI 10.1371/journal.pone.0161120 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DV4EG UT WOS:000382877400017 PM 27580176 ER PT J AU Pridgeon, JW Klesius, PH AF Pridgeon, Julia W. Klesius, Phillip H. TI Development of live attenuated Streptococcus agalactiae as potential vaccines by selecting for resistance to sparfloxacin (Retraction of vol 31, pg 2705, 2013) SO VACCINE LA English DT Correction C1 [Pridgeon, Julia W.; Klesius, Phillip H.] USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. RP Pridgeon, JW (reprint author), USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM Julia.Pridgeon@ars.usda.gov NR 1 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD AUG 31 PY 2016 VL 34 IS 38 BP 4642 EP 4642 DI 10.1016/j.vaccine.2016.07.043 PG 1 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DV5XU UT WOS:000383004800029 PM 27553749 ER PT J AU Adhikari, BN Bandyopadhyay, R Cotty, PJ AF Adhikari, Bishwo N. Bandyopadhyay, Ranajit Cotty, Peter J. TI Degeneration of aflatoxin gene clusters in Aspergillus flavus from Africa and North America SO AMB EXPRESS LA English DT Article DE Aspergillus flavus; Aflatoxin gene cluster; Non-aflatoxigenic; Cluster degeneration; Biocontrol; Evolution ID VEGETATIVE COMPATIBILITY GROUPS; POLYKETIDE SYNTHASE GENE; BIOLOGICAL-CONTROL; SECTION FLAVI; BIOSYNTHESIS GENES; FLANKING REGIONS; UNITED-STATES; CONTAMINATION; PARASITICUS; STRAINS AB Aspergillus flavus is the most common causal agent of aflatoxin contamination of food and feed. However, aflatoxinproducing potential varies widely among A. flavus genotypes with many producing no aflatoxins. Some nonaflatoxigenic genotypes are used as biocontrol agents to prevent contamination. Aflatoxin biosynthesis genes are tightly clustered in a highly conserved order. Gene deletions and presence of single nucleotide polymorphisms (SNPs) in aflatoxin biosynthesis genes are often associated with A. flavus inability to produce aflatoxins. In order to identify mechanisms of non-aflatoxigenicity in non-aflatoxigenic genotypes of value in aflatoxin biocontrol, complete cluster sequences of 35 A. flavus genotypes from Africa and North America were analyzed. Inability of some genotypes to produce aflatoxin resulted from deletion of biosynthesis genes. In other genotypes, non-aflatoxigenicity originated from SNP formation. The process of degeneration differed across the gene cluster; genes involved in early biosynthesis stages were more likely to be deleted while genes involved in later stages displayed high frequencies of SNPs. Comparative analyses of aflatoxin gene clusters provides insight into the diversity of mechanisms of nonaflatoxigenicity in A. flavus genotypes used as biological control agents. The sequences provide resources for both diagnosis of non-aflatoxigenicity and monitoring of biocontrol genotypes during biopesticide manufacture and in the environment. C1 [Adhikari, Bishwo N.; Cotty, Peter J.] Univ Arizona, USDA ARS, Sch Plant Sci, 303 Forbes Bldg,POB 210036, Tucson, AZ 85721 USA. [Bandyopadhyay, Ranajit] IITA, PMB 5320,Oyo Rd, Ibadan, Nigeria. RP Cotty, PJ (reprint author), Univ Arizona, USDA ARS, Sch Plant Sci, 303 Forbes Bldg,POB 210036, Tucson, AZ 85721 USA. EM pjcotty@email.arizona.edu OI Adhikari, Bishwo/0000-0003-4840-9609 FU Agricultural Research Service, US Department of Agriculture, CRIS [5347-42000-019-00D] FX The funding for this work was provided by Agricultural Research Service, US Department of Agriculture, CRIS project 5347-42000-019-00D. NR 90 TC 1 Z9 1 U1 14 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2191-0855 J9 AMB EXPRESS JI AMB Express PD AUG 31 PY 2016 VL 6 AR 62 DI 10.1186/s13568-016-0228-6 PG 16 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DU6WJ UT WOS:000382355000001 PM 27576895 ER PT J AU Cirilli, M Geuna, F Babini, AR Bozhkova, V Catalano, L Cavagna, B Dallot, S Decroocq, V Dondini, L Foschi, S Ilardi, V Liverani, A Mezzetti, B Minafra, A Pancaldi, M Pandolfini, T Pascal, T Savino, VN Scorza, R Verde, I Bassi, D AF Cirilli, Marco Geuna, Filippo Babini, Anna R. Bozhkova, Valentina Catalano, Luigi Cavagna, Beniamino Dallot, Sylvie Decroocq, Veronique Dondini, Luca Foschi, Stefano Ilardi, Vincenza Liverani, Alessandro Mezzetti, Bruno Minafra, Angelantonio Pancaldi, Marco Pandolfini, Tiziana Pascal, Thierry Savino, Vito N. Scorza, Ralph Verde, Ignazio Bassi, Daniele TI Fighting Sharka in Peach: Current Limitations and Future Perspectives SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE genetic engineering; fruit breeding; PPV virus; Prunus persica (L.) Batsch; RNAi ID PLUM-POX-VIRUS; PRUNUS-DAVIDIANA; HAIRPIN-RNA; DISEASE RESISTANCE; DIDERON ISOLATE; PLANTS; INFECTION; DOMESTICA; PROTEIN; GENES AB Sharka, caused by Plum Pox Virus (PPV), is by far the most important infectious disease of peach [P persica (L) Batsch] and other Prunus species. The progressive spread of the virus in many important growing areas throughout Europe poses serious issues to the economic sustainability of stone fruit crops, peach in particular. The adoption of internationally agreed upon rules for diagnostic tests, strain-specific monitoring schemes and spatial temporal modeling of virus spread, are all essential for a more effective sharka containment. The EU regulations on nursery activity should be modified based on the zone delimitation of PPV presence, limiting open-field production of propagation materials only to virus free areas. Increasing the efficiency of preventive measures should be augmented by the short-term development of resistant cultivars. Putative sources of resistance/tolerance have been recently identified in peach germplasm, although the majority of novel resistant sources to PPV-M have been found in almond. However, the complexity of introgression from related species imposes the search for alternative strategies. The use of genetic engineering, particularly RNA interference (RNAi)-based approaches, appears as one of the most promising perspectives to introduce a durable resistance to PPV in peach germplasm, notwithstanding the well-known difficulties of in vitro plant regeneration in this species. In this regard, rootstock transformation to induce RNAi-mediated systemic resistance would avoid the transformation of numerous commercial cultivars, and may alleviate consumer resistance to the use of GM plants. C1 [Cirilli, Marco; Geuna, Filippo; Bassi, Daniele] Univ Milan, Dept Agr & Environm Sci DISAA, Milan, Italy. [Babini, Anna R.] Reg Emilia Romagna, Phytosanit Serv, Bologna, Italy. [Bozhkova, Valentina] Fruit Growing Inst, Dept Breeding Genet Resources & Biotechnol, Plovdiv, Bulgaria. [Catalano, Luigi] Ctr Interprofess Attivita Vivaist, Rome, Italy. [Cavagna, Beniamino] Reg Lombardia, Phytosanit Serv, Milan, Italy. [Dallot, Sylvie] INRA, BGPI, UMR 385, Montpellier, France. [Decroocq, Veronique] Univ Bordeaux, INRA, UMR Biol Fruit & Pathol 1332, Villenave Domon, France. [Dondini, Luca] Univ Bologna, Dipartimento Sci Agr, Bologna, Italy. [Foschi, Stefano] Ctr Ric Prod Vegetali, Cesena, Italy. [Ilardi, Vincenza] Ctr Ric Patol Vegetale, Consiglio Ric Agr & Anal Econ Agr, Rome, Italy. [Liverani, Alessandro] Consiglio Ric Agr & Anal Econ Agr, Unita Ric Frutticoltura Forli, Forli, Italy. [Mezzetti, Bruno] Univ Politecn Marche, Dipartimento Sci Agr Alimentari & Ambientali, Ancona, Italy. [Minafra, Angelantonio] CNR, IPSP, Bari, Italy. [Pancaldi, Marco] Ctr Attivita Vivaist, Faenza, Italy. [Pandolfini, Tiziana] Univ Verona, Dept Biotechnol, Verona, Italy. [Pascal, Thierry] INRA, GAFL UR1052, Montfavet, France. [Savino, Vito N.] Univ Bari Aldo Moro, DiSSPA, Bari, Italy. [Scorza, Ralph] USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. [Verde, Ignazio] Consiglio Ric Agr Anal & Econ Agr, Ctr Ric Frutticoltura, Rome, Italy. RP Bassi, D (reprint author), Univ Milan, Dept Agr & Environm Sci DISAA, Milan, Italy. EM daniele.bassi@unimi.it FU Apofruit; BattistiniVivai; CAV; CIVI CRPV; Europfruit; GeoplantVivai; MinguzziSpA; New Plant; Orogel Fresco; Pempacorer; University of Milan; Vitroplant; ZaniGranfrutta; ZanziFiliVivai FX The International Workshop was funded by the following Italian subjects: Apofruit, BattistiniVivai, CAV, CIVI CRPV, Europfruit, GeoplantVivai, MinguzziSpA, New Plant, Orogel Fresco, Pempacorer, University of Milan, Vitroplant, ZaniGranfrutta, ZanziFiliVivai. NR 51 TC 0 Z9 0 U1 13 U2 13 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD AUG 30 PY 2016 VL 7 AR 1290 DI 10.3389/fpls.2015.01290 PG 8 WC Plant Sciences SC Plant Sciences GA DU3OY UT WOS:000382122800001 PM 27625664 ER PT J AU Brantley, ST Schulte, ML Bolstad, PV Miniat, CF AF Brantley, Steven T. Schulte, Morgan L. Bolstad, Paul V. Miniat, Chelcy F. TI Equations for Estimating Biomass, Foliage Area, and Sapwood of Small Trees in the Southern Appalachians SO FOREST SCIENCE LA English DT Article DE allometric equations; forest regeneration; forest understories; leaf area index; plant surface area; sapwood area ID ALLOMETRIC EQUATIONS; ABOVEGROUND BIOMASS; BOREAL FOREST; LEAF-AREA; ECOSYSTEM; PATTERNS; PINE; TRANSPIRATION; RESPIRATION; ALLOCATION AB Small trees and shrubs play an important role in forest diversity and regeneration and may contribute substantially to ecosystem fluxes of carbon and water; however, relatively little attention is given to quantifying the contribution of small trees to forest processes. One reason for this may be that the allometric equations developed for large trees tend to systematically underestimate structural variables such as biomass and foliage area when applied to small trees, both on an individual tree level and at the stand level. To test this hypothesis, we developed allometric equations for trees < 10 cm dbh (D) for seven dependent variables (woody, foliage, and total biomass; woody, foliage, and whole-plant surface area; and sapwood area) and compared these new equations with the existing equations for large trees. We found significant differences between small tree and large tree equations for most variables and showed that equations for large trees tend to underestimate the structural characteristics of small trees. When we applied new size-specific equations to forest survey data representing a chronosequence of forest development, estimates of small tree biomass increased 30-73% and estimates of foliage area increased 72-142% compared with results using only equations for large trees. These results suggest that small trees can contribute substantially to forest structure and associated ecosystem fluxes, especially in stands with a large proportion of small trees. However, size-specific equations for small trees did not substantially change the estimates of forest biomass in these stands, and the need to develop size-specific equations may depend on the variables of interest. C1 [Brantley, Steven T.] Joseph W Jones Ecol Res Ctr, Ecohydrol, Newton, GA 39870 USA. [Schulte, Morgan L.; Miniat, Chelcy F.] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Laboratory, Washington, DC 20250 USA. [Bolstad, Paul V.] Univ Minnesota, Dept Forest Resources, Minneapolis, MN 55455 USA. RP Brantley, ST (reprint author), Joseph W Jones Ecol Res Ctr, Ecohydrol, Newton, GA 39870 USA. EM sbrantle@jonesctr.org FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2012-67019-19484]; US Forest Service/University of Minnesota cooperative [12-CS-11330140-128]; National Science Foundation [DEB0218001, DEB0823293] FX This study was supported by Agriculture and Food Research Initiative Competitive Grant 2012-67019-19484 from the USDA National Institute of Food and Agriculture to the USDA Forest Service Southern Research Station and US Forest Service/University of Minnesota cooperative agreement 12-CS-11330140-128. National Science Foundation Grants DEB0218001 and DEB0823293 to the Coweeta LTER program at the University of Georgia provided logistical support. We thank E. Quinlan of the Highlands Biological Station for help with field data collection and M. Conner for advice on statistical analyses. We are also grateful to K. Elliott and D. Young for providing comments on a previous version of this article. We acknowledge the support of the staff at Coweeta Hydrologic Laboratory, especially D. Fowler and C. Brown for logistical support. NR 38 TC 0 Z9 0 U1 12 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD AUG 30 PY 2016 VL 62 IS 4 BP 414 EP 421 DI 10.5849/forsci.15-041 PG 8 WC Forestry SC Forestry GA DW1JP UT WOS:000383399800007 ER PT J AU Ganey, JL Iniguez, JM Hedwall, S Block, WM Ward, JP Jonnes, RS Rawlinson, TA Kyle, SC Apprill, DL AF Ganey, Joseph L. Iniguez, Jose M. Hedwall, Shaula Block, William M. Ward, James P., Jr. Jonnes, Ryan S. Rawlinson, Todd A. Kyle, Sean C. Apprill, Darrell L. TI Evaluating Desired Conditions for Mexican Spotted Owl Nesting and Roosting Habitat SO FOREST SCIENCE LA English DT Article DE adaptive management; basal area; canopy cover; large trees; management recommendations; Mexican spotted owl; recovery planning; Strix occidentalis lucida ID SACRAMENTO MOUNTAINS AB The Mexican spotted owl (Strix occidentalis lucida) was listed as a threatened species in 1993, primarily because of concerns over the loss of late seral forest habitat to timber harvest and wildfire. A recovery plan prepared for this owl subspecies explicitly assumed that nesting (and/or roosting) habitat was a primary factor limiting distribution of Mexican spotted owls and provided four desired conditions for identifying and managing potential owl nesting/roosting habitat in forested habitat. We used data collected at nest sites of Mexican spotted owls in the Sacramento Mountains, New Mexico, to evaluate how well these desired conditions and associated forest structural attributes described nesting habitat in this area. All nest sites included in our analyses successfully fledged young during the study. These nest sites generally featured higher levels of the structural attributes included in the desired conditions (total basal area, density of trees >46 cm in dbh, percentage of basal area in trees 30-46 cm dbh, and percentage of basal area in trees >46 cm dbh) than the surrounding stand, yet only 46-87% of sampled nest sites met single desired conditions and only 22% met all four conditions simultaneously. The best generalized linear models using combinations of these four structural attributes plus canopy cover to distinguish between nest sites and random sites within owl home ranges all contained canopy cover and percentage of basal area in trees >46 cm dbh. Relative importance values were high for both of these attributes (1.000 and 0.983, respectively), and confidence intervals around parameter estimates included zero for all other attributes. The present combination of four desired conditions did not consistently identify nesting habitat in this area, required managing for levels of structural attributes that were greater than levels typically observed at successful owl nest sites, and did not include canopy cover, which was the single best predictor in the Sacramento Mountains. We recommend revising the desired conditions in the Sacramento Mountains to emphasize canopy cover and some attribute measuring the large tree component. We also recommend repeating this assessment in other geographic areas to determine how well the desired conditions for those areas describe nesting habitat for owls. C1 [Ganey, Joseph L.; Iniguez, Jose M.; Block, William M.] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Hedwall, Shaula; Ward, James P., Jr.] US Fish & Wildlife Serv, Washington, DC USA. [Jonnes, Ryan S.; Kyle, Sean C.] Western Assoc Fish & Wildlife Agencies, Boise, ID USA. [Rawlinson, Todd A.; Apprill, Darrell L.] US Forest Serv, USDA, Lincoln Natl Forest, Flagstaff, AZ USA. RP Ganey, JL (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. EM jganey@fs.fed.us; jiniguez@fs.fed.us; shaula_hedwall@fws.gov; wblock@fs.fed.us; patrick_ward@fws.gov; ryansjonnes@gmail.com; trawlinson@fs.fed.us; sean.kyle@wafwa.org; darrellapprill@gmail.com FU Southwestern Region, USDA Forest Service; Lincoln National Forest and Rocky Mountain Research Station, USDA Forest Service FX We thank the many dedicated biologists who assisted with locating Mexican spotted owl nests in the Sacramento Mountains over the years, as well as the members of the Mexican spotted owl Recovery Team, past and present, for their efforts on behalf of the owl. For assistance with sampling nest sites, we thank T. Borneman, J. Cannon, C. Cobb, M. Collado, R. Crandall, C. Edge, L. Gedacht, J. Gorey, J. Goyette, S. Halsey, D. Harrington, T. Heard, M. Hillman, M. Ihnken, A. Mahoney, C. Mosby, L. Navarrete, C. Okraska, H. Oswald, M. Peterson, R. Peterson, E. Pollom, B. Rubeck, A. Salonikios, N. Unsworth, A. VandeVoort, N. von Hedeman, K. Wagner, and A. Walters. K. Hamilton prepared the figure showing plot layout. We thank the personnel from the Sacramento Ranger District, USDA Forest Service (especially M. Mauter, J. Montoya, R. Guaderrama, D. Salas, and J. Williams), for logistical support during the study. Major funding was provided by the Southwestern Region, USDA Forest Service, with additional funding from the Lincoln National Forest and Rocky Mountain Research Station, USDA Forest Service. We thank D. DeLorenzo (USDA Forest Service, Southwestern Region) for his support and assistance with securing funding throughout the study, and K. Blatner and three anonymous reviewers for their comments on an earlier version of this article. NR 22 TC 0 Z9 0 U1 9 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD AUG 30 PY 2016 VL 62 IS 4 BP 457 EP 462 DI 10.5849/forsci.15-027 PG 6 WC Forestry SC Forestry GA DW1JP UT WOS:000383399800012 ER PT J AU Riley, R Haridas, S Wolfe, KH Lopes, MR Hittinger, CT Goker, M Salamov, AA Wisecaver, JH Long, TM Calvey, CH Aerts, AL Barry, KW Choi, C Clum, A Coughlan, AY Deshpande, S Douglass, AP Hanson, SJ Klenk, HP LaButti, KM Lapidus, A Lindquist, EA Lipzen, AM Meier-Kolthoff, JP Ohm, RA Otillar, RP Pangilinan, JL Peng, Y Rokas, A Rosa, CA Scheuner, C Sibirny, AA Slot, JC Stielow, JB Sun, H Kurtzman, CP Blackwell, M Grigoriev, IV Jeffries, TW AF Riley, Robert Haridas, Sajeet Wolfe, Kenneth H. Lopes, Mariana R. Hittinger, Chris Todd Goeker, Markus Salamov, Asaf A. Wisecaver, Jennifer H. Long, Tanya M. Calvey, Christopher H. Aerts, Andrea L. Barry, Kerrie W. Choi, Cindy Clum, Alicia Coughlan, Aisling Y. Deshpande, Shweta Douglass, Alexander P. Hanson, Sara J. Klenk, Hans-Peter LaButti, Kurt M. Lapidus, Alla Lindquist, Erika A. Lipzen, Anna M. Meier-Kolthoff, Jan P. Ohm, Robin A. Otillar, Robert P. Pangilinan, Jasmyn L. Peng, Yi Rokas, Antonis Rosa, Carlos A. Scheuner, Carmen Sibirny, Andriy A. Slot, Jason C. Stielow, J. Benjamin Sun, Hui Kurtzman, Cletus P. Blackwell, Meredith Grigoriev, Igor V. Jeffries, Thomas W. TI Comparative genomics of biotechnologically important yeasts SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE genomics; bioenergy; biotechnological yeasts; genetic code; microbiology ID HORIZONTAL GENE-TRANSFER; SACCHAROMYCES-CEREVISIAE; PICHIA-STIPITIS; EVOLUTION; CODE; SEQUENCE; MECHANISM; PATHWAY; COMPLEX; ORIGIN AB Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Ourwell-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation. C1 [Riley, Robert; Haridas, Sajeet; Salamov, Asaf A.; Aerts, Andrea L.; Barry, Kerrie W.; Choi, Cindy; Clum, Alicia; Deshpande, Shweta; LaButti, Kurt M.; Lapidus, Alla; Lindquist, Erika A.; Lipzen, Anna M.; Ohm, Robin A.; Otillar, Robert P.; Pangilinan, Jasmyn L.; Peng, Yi; Sun, Hui; Grigoriev, Igor V.] Joint Genome Inst, Dept Energy, Walnut Creek, CA 94598 USA. [Wolfe, Kenneth H.; Coughlan, Aisling Y.; Douglass, Alexander P.; Hanson, Sara J.] Univ Coll Dublin, Sch Med, Conway Inst, Dublin 4, Ireland. [Lopes, Mariana R.; Hittinger, Chris Todd] Univ Wisconsin, Genet Biotechnol Ctr, Lab Genet, Madison, WI 53706 USA. [Lopes, Mariana R.; Rosa, Carlos A.] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. [Hittinger, Chris Todd] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Dept Energy, Madison, WI 53726 USA. [Goeker, Markus; Klenk, Hans-Peter; Meier-Kolthoff, Jan P.; Scheuner, Carmen; Stielow, J. Benjamin] Leibniz Inst, Deutsch Sammlung Mikroorganismen & Zellkulturen, D-38124 Braunschweig, Germany. [Wisecaver, Jennifer H.; Rokas, Antonis] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. [Long, Tanya M.; Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Long, Tanya M.] USDA, Forest Prod Lab, Madison, WI 53726 USA. [Calvey, Christopher H.] Xylome Corp, Madison, WI 53719 USA. [Klenk, Hans-Peter] Newcastle Univ, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. [Sibirny, Andriy A.] Natl Acad Sci Ukraine, Inst Cell Biol, Dept Mol Genet & Biotechnol, UA-79005 Lvov, Ukraine. [Sibirny, Andriy A.] Univ Rzeszow, Dept Biotechnol & Microbiol, PL-35601 Rzeszow, Poland. [Slot, Jason C.] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. [Stielow, J. Benjamin] Royal Netherlands Acad Arts & Sci, Cent Bur Schimmelcultures Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands. [Kurtzman, Cletus P.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Blackwell, Meredith] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Blackwell, Meredith] Univ South Carolina, Dept Biol Sci, Columbia, SC 29208 USA. [Lapidus, Alla] St Petersburg State Univ, Ctr Algorithm Biotechnol, St Petersburg 199004, Russia. [Ohm, Robin A.] Univ Utrecht, Dept Biol, Microbiol, NL-3508 Utrecht, Netherlands. RP Grigoriev, IV (reprint author), Joint Genome Inst, Dept Energy, Walnut Creek, CA 94598 USA.; Jeffries, TW (reprint author), Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. EM IVGrigoriev@lbl.gov; twjeffri@wisc.edu RI Ohm, Robin/I-6689-2016; OI Calvey, Christopher/0000-0002-7330-4983; Meier-Kolthoff, Jan Philipp/0000-0001-9105-9814; Wolfe, Kenneth/0000-0003-4992-4979 FU Office of Science of the US DOE [DE-AC02-05CH11231]; National Science Foundation [DEB-1442148, DEB-0072741, 0417180]; DOE Great Lakes Bioenergy Research Center; DOE Office of Science Grant [BER DE-FC02-07ER64494]; US Department of Agriculture (USDA) National Institute of Food and Agriculture Hatch Project [1003258]; European Research Council Grant [268893]; Science Foundation Ireland Grant [13/IA/1910]; Wellcome Trust; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [7371/13-6]; Pew Charitable Trusts; Alexander von Humboldt Foundation; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq; DOE Great Lakes Bioenergy Research Center DOE Office of Science Grant [BER DE-FC02-07ER64494]; USDA, Forest Products Laboratory FX We thank Marco A. Soares for computational advice. K.H.W. thanks G. Cagney, E. Dillon, and K. Wynne (University College Dublin Conway Institute Proteomics Core Facility) for help with MS. M.B. thanks Drs. S. O. Suh, H. Urbina, and N. H. Nguyen and numerous Louisiana State University undergraduates for their assistance. The work conducted by the US Department of Energy (DOE) Joint Genome Institute, a DOE Office of Science User Facility, is supported by Office of Science of the US DOE Contract DE-AC02-05CH11231. This material is based on work supported by National Science Foundation Grant DEB-1442148 (to C.T.H. and C.P.K.) and supported in part by DOE Great Lakes Bioenergy Research Center, DOE Office of Science Grant BER DE-FC02-07ER64494, and US Department of Agriculture (USDA) National Institute of Food and Agriculture Hatch Project 1003258. K.H.W. acknowledges European Research Council Grant 268893, Science Foundation Ireland Grant 13/IA/1910, and the Wellcome Trust. M.R.L. acknowledges a fellowship from the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (process no. 7371/13-6). C.T.H. is a Pew Scholar in the Biomedical Sciences and an Alfred Toepfer Faculty Fellow, which are supported by the Pew Charitable Trusts and the Alexander von Humboldt Foundation, respectively. C.A.R. acknowledges support from the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPq. Funding from National Science Foundation Grants DEB-0072741 (to M.B.) and 0417180 (to M.B.) supported discovery and study of many new yeast strains that contributed to this study. T.W.J. acknowledges DOE Great Lakes Bioenergy Research Center DOE Office of Science Grant BER DE-FC02-07ER64494 and the USDA, Forest Products Laboratory for financial support. NR 47 TC 7 Z9 7 U1 14 U2 14 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD AUG 30 PY 2016 VL 113 IS 35 BP 9882 EP 9887 DI 10.1073/pnas.1603941113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DV7BL UT WOS:000383090700064 PM 27535936 ER PT J AU Concha, C Palavesam, A Guerrero, FD Sagel, A Li, F Osborne, JA Hernandez, Y Pardo, T Quintero, G Vasquez, M Keller, GP Phillips, PL Welch, JB McMillan, WO Skoda, SR Scott, MJ AF Concha, Carolina Palavesam, Azhahianambi Guerrero, Felix D. Sagel, Agustin Li, Fang Osborne, Jason A. Hernandez, Yillian Pardo, Trinidad Quintero, Gladys Vasquez, Mario Keller, Gwen P. Phillips, Pamela L. Welch, John B. Owen McMillan, W. Skoda, Steven R. Scott, Maxwell J. TI A transgenic male-only strain of the New World screwworm for an improved control program using the sterile insect technique SO BMC BIOLOGY LA English DT Article DE New World screwworm; Sterile insect technique; tTA; Transformer; Male-only; Genetic control ID ENGINEERED MALE MOSQUITOS; FLY LUCILIA-CUPRINA; COCHLIOMYIA-HOMINIVORAX; SEXING SYSTEM; FRUIT-FLY; DIPTERA; CALLIPHORIDAE; RELEASE; FLIES; ERADICATION AB Background: The New World screwworm, Cochliomyia hominivorax, is a devastating pest of livestock endemic to subtropical and tropical regions of the Western hemisphere. The larvae of this species feed on the tissue of living animals, including man, and can cause death if untreated. Over 60 years ago, the sterile insect technique ( SIT) was developed with the aim of eradicating this pest, initially from Florida but subsequently from all of North and Central America. From the outset it was appreciated that SIT would be more efficient if only sterile males were released in the field, but this was not possible until now. Results: Here, we report on the development and evaluation of the first sexing strains of C. hominivorax that produce only males when raised on diet without tetracycline. Transgenic lines have been developed that possess a tetracycline repressible female-lethal genetic system. Ten of these lines show high female lethality at the late larval/pupal stages and three of them present dominant female lethality. Most of the lines were comparable to the wild type parental strain in several fitness parameters that are relevant to mass rearing in a production facility. Further, three lines performed well in male mating success and male competition assays, suggesting they would be sexually competitive in the field. Consequently, one transgenic line has been selected by the New World Screwworm Program for evaluation under mass rearing conditions. Conclusions: We conclude that the promising characteristics of the selected sexing strains may contribute to reduce production costs for the existing eradication program and provide more efficient population suppression, which should make a genetic control program more economical in regions were C. hominivorax remains endemic. C1 [Concha, Carolina; Li, Fang; Scott, Maxwell J.] North Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA. [Concha, Carolina; Hernandez, Yillian] Panama US Commiss Eradicat & Prevent Screwworm CO, Pacora, Panama. [Concha, Carolina; Owen McMillan, W.] Smithsonian Trop Res Inst, Naos Mol Lab, Panama City, Panama. [Palavesam, Azhahianambi; Guerrero, Felix D.] USDA ARS, Tick & Biting Fly Res Unit, Knipling Bushland Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Sagel, Agustin; Pardo, Trinidad; Quintero, Gladys; Vasquez, Mario; Phillips, Pamela L.; Skoda, Steven R.] USDA ARS, Screwworm Res Unit, Pacora, Panama. [Osborne, Jason A.] North Carolina State Univ, Dept Stat, Campus Box 8203, Raleigh, NC 27695 USA. [Keller, Gwen P.] USDA APHIS IS, Pacora, Panama. [Phillips, Pamela L.] USDA ARS, Screwworm Res Unit, Knipling Bushland Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Welch, John B.] USDA APHIS, IS Act Programs, 2881 F&B Rd, College Stn, TX 77845 USA. [Palavesam, Azhahianambi] Tamil Nadu Vet & Anim Sci Univ, Dept Vet Parasitol, Madras Vet Coll, Madras, Tamil Nadu, India. RP Scott, MJ (reprint author), North Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA. EM mjscott3@ncsu.edu FU USDA-ARS; NCSU; Biotechnology Risk Assessment Grant Program from the USDA National Institute of Food and Agriculture [2011-33522-30730]; Panama-United States Commission for the Eradication and Prevention of Screwworm (COPEG) FX Funding is gratefully acknowledged from specific cooperative agreements between the USDA-ARS and NCSU, Biotechnology Risk Assessment Grant Program competitive grant no. 2011-33522-30730 from the USDA National Institute of Food and Agriculture, the Panama-United States Commission for the Eradication and Prevention of Screwworm (COPEG) and start-up funds to MJS from NCSU. NR 40 TC 1 Z9 1 U1 14 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1741-7007 J9 BMC BIOL JI BMC Biol. PD AUG 30 PY 2016 VL 14 AR 72 DI 10.1186/s12915-016-0296-8 PG 13 WC Biology SC Life Sciences & Biomedicine - Other Topics GA DU5QA UT WOS:000382265200002 PM 27576512 ER PT J AU DeDonder, KD Harhay, DM Apley, MD Lubbers, BV Clawson, ML Schuller, G Harhay, GP White, BJ Larson, RL Capik, SF Riviere, JE Kalbfleisch, T Tessman, RK AF DeDonder, Keith D. Harhay, Dayna M. Apley, Michael D. Lubbers, Brian V. Clawson, Michael L. Schuller, Gennie Harhay, Gregory P. White, Brad J. Larson, Robert L. Capik, Sarah F. Riviere, Jim E. Kalbfleisch, Ted Tessman, Ronald K. TI Observations on macrolide resistance and susceptibility testing performance in field isolates collected from clinical bovine respiratory disease cases SO VETERINARY MICROBIOLOGY LA English DT Article DE Gamithromycin; Macrolide; Antimicrobial resistance; Bovine respiratory disease; Mannheimia haemolytica; Pasteurella multocida; Histophilus somni ID CONJUGATIVE ELEMENT ICE; MINIMUM INHIBITORY CONCENTRATIONS; PASTEURELLA-MULTOCIDA; MANNHEIMIA-HAEMOLYTICA; ANTIMICROBIAL SUSCEPTIBILITY; INFLAMMATORY MEDIATORS; ESCHERICHIA-COLI; LEUKOTRIENE B-4; TULATHROMYCIN; PATHOGENS AB The objectives of this study were; first, to describe gamithromycin susceptibility of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from cattle diagnosed with bovine respiratory disease (BRD) and previously treated with either gamithromycin for control of BRD (mass medication = MM) or sham-saline injected (control = CON); second, to describe the macrolide resistance genes present in genetically typed M. haemolytica isolates; third, use whole-genome sequencing (WGS) to correlate the phenotypic resistance and genetic determinants for resistance among M. haemolytica isolates. M. haemolytica (n = 276), P. multocida (n = 253), and H. somni (n = 78) were isolated from feedlot cattle diagnosed with BRD. Gamithromycin susceptibility was determined by broth microdilution. Whole-genome sequencing was utilized to determine the presence/absence of macrolide resistance genes and to genetically type M. haemolytica. Generalized linear mixed models were built for analysis. There was not a significant difference between MM and CON groups in regards to the likelihood of culturing a resistant isolate of M. haemolytica or P. multocida. The likelihood of culturing a resistant isolate of M. haemolytica differed significantly by state of origin in this study. A single M. haemolytica genetic subtype was associated with an over whelming majority of the observed resistance. H. somni isolation counts were low and statistical models would not converge. Phenotypic resistance was predicted with high sensitivity and specificity by WGS. Additional studies to elucidate the relationships between phenotypic expression of resistance/genetic determinants for resistance and clinical response to antimicrobials are necessary to inform judicious use of antimicrobials in the context of relieving animal disease and suffering. (C) 2016 Elsevier B.V. All rights reserved. C1 [DeDonder, Keith D.; Capik, Sarah F.] Kansas State Univ, Coll Vet Med, Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Harhay, Dayna M.; Clawson, Michael L.; Schuller, Gennie; Harhay, Gregory P.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Apley, Michael D.; White, Brad J.; Larson, Robert L.] Kansas State Univ, Coll Vet Med, Clin Sci, Manhattan, KS 66506 USA. [Lubbers, Brian V.] Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, Manhattan, KS 66506 USA. [Riviere, Jim E.] Kansas State Univ, Coll Vet Med, Inst Computat Comparat Med, Manhattan, KS 66506 USA. [Kalbfleisch, Ted] Univ Louisville, Sch Med, Biochem & Mol Genet Dept, Louisville, KY 40292 USA. [Tessman, Ronald K.] Merial, Pharmaceut Res & Dev, Duluth, GA USA. [DeDonder, Keith D.] Vet & Biomed Res Ctr Inc, Manhattan, KS USA. RP DeDonder, KD (reprint author), Kansas State Univ, Coll Vet Med, Diagnost Med Pathobiol, Manhattan, KS 66506 USA.; DeDonder, KD (reprint author), Vet & Biomed Res Ctr Inc, Manhattan, KS USA. EM keith@mvsinc.net OI DeDonder, Keith/0000-0003-1667-1346 FU Merial, Duluth, Georgia, United States FX This study was financially supported, in part, by Merial, Duluth, Georgia, United States. NR 40 TC 1 Z9 1 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD AUG 30 PY 2016 VL 192 BP 186 EP 193 DI 10.1016/j.vetmic.2016.07.021 PG 8 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA DU7TE UT WOS:000382416700025 PM 27527782 ER PT J AU Gagne, RJ AF Gagne, Raymond J. TI Three new genera and three new species of Nearctic Lasiopteridi (Diptera: Cecidomyiidae: Cecidomyiinae) from Asteraceae and Caprifoliaceae, and the tribe Rhopalomyiini subsumed under Oligotrophini SO ZOOTAXA LA English DT Article DE gall midges; Helianthus; Lonicera; Symphoricarpos; Oligotrophini; Lasiopteridi AB Three new Nearctic genera of gall midges (Diptera: Cecidomyiidae), each with a new species, are described: Helianthecis Gagne for Helianthecis capitum Gagne new species, that lives in flower heads of Helianthus spp. (Asteraceae) from North Dakota to Texas; Lonicerae Gagne for Lonicerae russoi Gagne, new species, and Lonicerae lonicera (Felt), new combination, that form bud galls on Lonicera spp. (Caprifoliaceae) in California; and Chiosperma Gagne for Chiosperma turgidum Gagne new species, that forms a bud gall on Symphoricarpos albus (L.) S.F. Blake (Caprifoliaceae) in Washington. The three new genera belong to the supertribe Lasiopteridi and are placed in the tribe Oligotrophini. The tribes Oligotrophini and Rhopalomyiini are combined. C1 [Gagne, Raymond J.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, MRC 168,POB 37012, Washington, DC 20013 USA. RP Gagne, RJ (reprint author), ARS, Systemat Entomol Lab, USDA, Smithsonian Inst, MRC 168,POB 37012, Washington, DC 20013 USA. EM raymond.gagne@ars.usda.gov NR 11 TC 0 Z9 0 U1 1 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD AUG 30 PY 2016 VL 4158 IS 3 BP 403 EP 418 DI 10.11646/zootaxa.4158.3.6 PG 16 WC Zoology SC Zoology GA DU1SE UT WOS:000381988500006 PM 27615893 ER PT J AU Cao, YZ Zavattieri, P Youngblood, J Moon, R Weiss, J AF Cao, Yizheng Zavattieri, Pablo Youngblood, Jeffrey Moon, Robert Weiss, Jason TI The relationship between cellulose nanocrystal dispersion and strength SO CONSTRUCTION AND BUILDING MATERIALS LA English DT Article DE Cellulose nanocrystal; Agglomeration; Ultrasonication; Flexural strength; Short circuit diffusion ID NANOTUBE-EPOXY COMPOSITES; CEMENT PASTE; PERCOLATION-THRESHOLD; CARBON NANOTUBES; AGGLOMERATION; PERFORMANCE; SIMULATION; NETWORKS; CONCRETE; PHASES AB This paper studies the agglomeration of cellulose nanocrystals (CNCs) and uses ultrasonication to disperse CNCs in cement pastes in an attempt to improve strength. Rheological measurements show that when the concentration of CNCs exceeds 1.35% by volume in deionized water, agglomerates start to develop. This experimental finding is comparable to the value obtained from a geometrical percolation model (1.38% by volume). When the matrix phase (deionized water) is replaced with a simulated cement paste pore solution, the CNCs begin to agglomerate at a lower concentration (approximately 0.18% by volume). The CNC concentration of 0.18% corresponds to the concentration of CNCs in cement paste where the maximum strength is reached. Tip ultrasonication was found to effectively disperse the CNCs and the cement pastes obtained strength improvements of up to 50%, which is significantly better than the strength improvement of raw CNCs alone (20-30%). (C) 2016 Elsevier Ltd. All rights reserved. C1 [Cao, Yizheng; Youngblood, Jeffrey] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Zavattieri, Pablo] Purdue Univ, Lyles Sch Civil Engn, W Lafayette, IN 47907 USA. [Moon, Robert] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Weiss, Jason] Oregon State Univ, Sch Civil & Construct Engn, Corvallis, OR 97331 USA. RP Weiss, J (reprint author), Oregon State Univ, Sch Civil & Construct Engn, Corvallis, OR 97331 USA. EM jason.weiss@oregonstate.edu OI Weiss, William/0000-0003-2859-7980 FU National Science Foundation United States through Award CMMI [1131596] FX This work was supported by the National Science Foundation United States through Award no. CMMI #1131596. The authors are grateful for the USFS Forest Products Laboratory for providing the CNC materials. We also thank Carlos Martinez for allowing the use of the rheometer. NR 41 TC 1 Z9 1 U1 14 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0950-0618 EI 1879-0526 J9 CONSTR BUILD MATER JI Constr. Build. Mater. PD AUG 30 PY 2016 VL 119 BP 71 EP 79 DI 10.1016/j.conbuildmat.2016.03.077 PG 9 WC Construction & Building Technology; Engineering, Civil; Materials Science, Multidisciplinary SC Construction & Building Technology; Engineering; Materials Science GA DQ1JF UT WOS:000378956600008 ER PT J AU Wang, YJ Xu, XD Liu, HZ Li, YQ Li, YH Hu, ZY Gao, XQ Ma, YM Sun, JH Lenschow, DH Zhong, SY Zhou, MY Bian, XD Zhao, P AF Wang, Yinjun Xu, Xiangde Liu, Huizhi Li, Yueqing Li, Yaohui Hu, Zeyong Gao, Xiaoqing Ma, Yaoming Sun, Jihua Lenschow, Donald H. Zhong, Shiyuan Zhou, Mingyu Bian, Xindi Zhao, Ping TI Analysis of land surface parameters and turbulence characteristics over the Tibetan Plateau and surrounding region SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article ID SENSIBLE HEAT-FLUX; WATER-VAPOR; SEMIARID AREA; LAYER; TERRAIN; MOMENTUM; ALBEDO; TOWER; CHINA AB Based on the results from 11 flux sites during the third Tibetan Plateau (TP) Experiment (TIPEX III), land surface parameters and the turbulence characteristics of the atmospheric surface layer over the TP and surrounding region are analyzed. Monin-Obukhov similarity theory has been used to calculate the aerodynamic roughness length z(0m) and the excess resistance to heat transfer kB(-1) = ln(z(0m)/z(0h)), and the factors that cause variations of z(0m) and kB(-1) are investigated. The main drivers for the diurnal variations of surface albedo (alpha) at different sites are solar elevation, solar radiation, and soil moisture. The eddy correlation method is utilized to inversely calculate bulk transfer coefficients for momentum (C-D) and heat (C-H) at different sites. The relationships between C-D and C-H and the wind speed at 10m follow a power law for unstable stratification. For stable stratification, both C-D and C-H increase with increasing wind speed when wind speed is less than 5 m/s. Diurnal variations of turbulent fluxes are compared at different sites, and the relationships between turbulent fluxes and other variables are analyzed. Wind speed variance normalized by the friction velocity (sigma(u)/u*, sigma(v)/u*, sigma(w)/u*) for neutral stratification (C-u1, C-v1, C-w1), and temperature and humidity variance normalized by a temperature and humidity scale (sigma(T)/T*, sigma(q)/q*) under free convection (z/L< -0.1) (C-T, C-q) are fitted with similarity relations. The differences in similarity constants (C-u1, C-v1, C-w1, C-T, C-q) at different sites are discussed. For stable stratification, cases are divided into weakly stable conditions and intermittent turbulence, and the critical values for these two states are determined. Shear and buoyancy terms in the turbulence kinetic energy equation for different stratifications are analyzed. C1 [Wang, Yinjun; Xu, Xiangde; Zhao, Ping] Chinese Acad Meteorol Sci, Beijing, Peoples R China. [Liu, Huizhi] Chinese Acad Sci, Inst Atmospher Phys, LAPC, Beijing, Peoples R China. [Li, Yueqing] China Meteorol Adm, Inst Plateau Meteorol, Chengdu, Peoples R China. [Li, Yaohui] China Meteorol Adm, Inst Arid Meteorol, Key Lab Arid Climat Change & Reducing Deserter Ga, Lanzhou, Peoples R China. [Hu, Zeyong; Gao, Xiaoqing] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou, Peoples R China. [Ma, Yaoming] Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing, Peoples R China. [Sun, Jihua] Meteorol Inst Yunnan Prov, Kunming, Peoples R China. [Lenschow, Donald H.] Natl Ctr Atmospher Res, POB 3000, Boulder, CO 80307 USA. [Zhong, Shiyuan] Michigan State Univ, E Lansing, MI 48824 USA. [Zhou, Mingyu] Natl Marine Environm Forecasting Ctr, Beijing, Peoples R China. [Zhou, Mingyu] China Meteorol Adm, Natl Climate Ctr, Beijing, Peoples R China. [Bian, Xindi] US Forest Serv, Northern Res Stn, USDA, E Lansing, MI USA. RP Wang, YJ (reprint author), Chinese Acad Meteorol Sci, Beijing, Peoples R China. EM pbl_wyj@sina.cn FU China special fund for meteorological research in the public interest [GYHY201406001]; National Key Technologies R&D Program of China [2012BAK10B04]; National Natural Science Foundation Of China [41130960]; National Science Foundation FX This study was supported by the China special fund for meteorological research in the public interest grant GYHY201406001, National Key Technologies R&D Program of China grant 2012BAK10B04, and the National Natural Science Foundation Of China grant 41130960. The National Center for Atmospheric Research is sponsored by the National Science Foundation. If the researchers want to access the third Tibetan Plateau Experiment for atmospheric sciences (TIPEX III) data used in this manuscript, they can contact author Yinjun Wang. E-mail: pbl_wyj@sina.cn. At the present time, the data can only be accessed from the scientific project after signing the agreement given by China Meteorological Administration. NR 41 TC 0 Z9 0 U1 5 U2 5 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD AUG 27 PY 2016 VL 121 IS 16 BP 9540 EP 9560 DI 10.1002/2016JD025401 PG 21 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DZ9OQ UT WOS:000386207200016 ER PT J AU Zhou, DC Li, D Sun, G Zhang, LX Liu, YQ Hao, L AF Zhou, Decheng Li, Dan Sun, Ge Zhang, Liangxia Liu, Yongqiang Hao, Lu TI Contrasting effects of urbanization and agriculture on surface temperature in eastern China SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article ID URBAN HEAT-ISLAND; LAND-COVER CHANGE; DEVELOPMENT INTENSITY GRADIENT; 32 MAJOR CITIES; UNITED-STATES; BIOGEOPHYSICAL IMPACTS; OBSERVATIONAL EVIDENCE; SPATIOTEMPORAL TRENDS; CLIMATE-CHANGE; TIME-SERIES AB The combined effect of urbanization and agriculture, two most pervasive land use activities, on the surface climate remains poorly understood. Using Moderate Resolution Imaging Spectroradiometer data over 2010-2015 and forests as reference, we showed that urbanization warmed the land surface temperature (LST), especially during the daytime and in growing seasons (maximized at 5.0 +/- 2.0 degrees C in May), whereas agriculture (dominated by double-cropping system) cooled the LST in two growing seasons during the daytime and all the months but July during the nighttime in Jiangsu Province, eastern China. Collectively, they had insignificant effects on the LST during the day (-0.01 degrees C) and cooled the LST by -0.6 degrees C at night. We also found large geographic variations associated with their thermal effects, indicated by a warming tendency southward. These spatiotemporal patterns depend strongly on vegetation activity, evapotranspiration, surface albedo, and the background climate. Our results emphasize the great potential of agriculture in offsetting the heating effects caused by rapid urbanization in China. C1 [Zhou, Decheng; Zhang, Liangxia; Hao, Lu] Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Nanjing, Jiangsu, Peoples R China. [Zhou, Decheng; Zhang, Liangxia; Hao, Lu] Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Nanjing, Jiangsu, Peoples R China. [Li, Dan] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC USA. [Liu, Yongqiang] US Forest Serv, Ctr Forest Disturbance Sci, Southern Res Stn, USDA, Athens, GA USA. RP Zhou, DC (reprint author), Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Nanjing, Jiangsu, Peoples R China.; Zhou, DC (reprint author), Nanjing Univ Informat Sci & Technol, Coll Appl Meteorol, Nanjing, Jiangsu, Peoples R China. EM zhoudc@nuist.edu.cn FU Natural Science Foundation of the Jiangsu Higher Education Institutions of China [15KJB170013]; National Natural Science Foundation of China [41501465]; Startup Foundation for Introducing Talent of NUIST [2014r051]; China Scholarship Council FX The MODIS data are provided by the Land Processes Distributed Active Archive Center (LP DAAC, https://lpdaac.usgs.gov/) managed by the NASA Earth Science Data and Information System (ESDIS) project. The Landsat 8 OLI and DEM data are provided by the U.S. Geological Survey's EROS Data Center (http://eros.usgs.gov/). The climate data are provided by WorldClim, University of California (http://www.worldclim.org/). This study was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (15KJB170013), the National Natural Science Foundation of China (41501465), and the Startup Foundation for Introducing Talent of NUIST (2014r051). Funding for the visit of the USDA Forest Service is provided by the China Scholarship Council. NR 89 TC 0 Z9 0 U1 13 U2 13 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD AUG 27 PY 2016 VL 121 IS 16 BP 9597 EP 9606 DI 10.1002/2016JD025359 PG 10 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DZ9OQ UT WOS:000386207200019 ER PT J AU Hadidi, A Flores, R Candresse, T Barba, M AF Hadidi, Ahmed Flores, Ricardo Candresse, Thierry Barba, Marina TI Next-Generation Sequencing and Genome Editing in Plant Virology SO FRONTIERS IN MICROBIOLOGY LA English DT Review DE next-generation sequencing; plant virology; plant viruses; viroids; resistance to plant viruses by CRISPR-Cas9 ID LEAF-CURL-VIRUS; COMPLETE NUCLEOTIDE-SEQUENCE; SPINDLE TUBER VIRUS; CRISPR-CAS SYSTEMS; SMALL-RNAS; DNA VIRUS; SIRNA BIOGENESIS; IMMUNE-SYSTEM; DWARF DISEASE; STRANDED-DNA AB Next-generation sequencing (NGS) has been applied to plant virology since 2009. NGS provides highly efficient, rapid, low cost DNA, or RNA high-throughput sequencing of the genomes of plant viruses and viroids and of the specific small RNAs generated during the infection process. These small RNAs, which cover frequently the whole genome of the infectious agent, are 21-24 nt long and are known as vsRNAs for viruses and vd-sRNAs for viroids. NGS has been used in a number of studies in plant virology including, but not limited to, discovery of novel viruses and viroids as well as detection and identification of those pathogens already known, analysis of genome diversity and evolution, and study of pathogen epidemiology. The genome engineering editing method, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been successfully used recently to engineer resistance to DNA geminiviruses (family, Geminiviridae) by targeting different viral genome sequences in infected Nicotiana bentham/ana or Arabidopsis plants. The DNA viruses targeted include tomato yellow leaf curl virus and merremia mosaic virus (begomovirus); beet curly top virus and beet severe curly top virus (curtovirus); and bean yellow dwarf virus (mastrevirus). The technique has also been used against the RNA viruses zucchini yellow mosaic virus, papaya ringspot virus and turnip mosaic virus (potyvirus) and cucumber vein yellowing virus (ipomovirus, family, Potyviridae) by targeting the translation initiation genes elF4E in cucumber or Arabidopsis plants. From these recent advances of major importance, it is expected that NGS and CRISPR-Cas technologies will play a significant role in the very near future in advancing the field of plant virology and connecting it with other related fields of biology. C1 [Hadidi, Ahmed] USDA ARS, Beltsville, MD 20705 USA. [Flores, Ricardo] Univ Politecn Valencia, Inst Biol Mol & Celular Plantas, CSIC, Valencia, Spain. [Candresse, Thierry] Univ Bordeaux, INRA, UMR Biol Fruit & Pathol 1332, Bordeaux, France. [Barba, Marina] Consiglio Ric Agr & Anal Econ Agr, Ctr Ric Patol Vegetale, Rome, Italy. RP Hadidi, A (reprint author), USDA ARS, Beltsville, MD 20705 USA. EM ahadidi@yahoo.com RI Flores, Ricardo/K-2788-2014 OI Flores, Ricardo/0000-0002-3033-5077 FU Spanish Ministerio de Economia y Competitividad [BEU2014-56812-P] FX Work in RF laboratory has been supported by the Spanish Ministerio de Economia y Competitividad (grant BEU2014-56812-P), and in MB laboratory by Consiglio per la Ricerca in Agricoltura e l'analisi dell'Economia Agraria (CREA). NR 168 TC 0 Z9 1 U1 56 U2 68 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD AUG 26 PY 2016 VL 7 AR 1325 DI 10.3389/fmicb.2016.01325 PG 12 WC Microbiology SC Microbiology GA DU0PH UT WOS:000381906500001 PM 27617007 ER PT J AU Williams, A Hunter, MC Kammerer, M Kane, DA Jordan, NR Mortensen, DA Smith, RG Snapp, S Davis, AS AF Williams, Alwyn Hunter, Mitchell C. Kammerer, Melanie Kane, Daniel A. Jordan, Nicholas R. Mortensen, David A. Smith, Richard G. Snapp, Sieglinde Davis, Adam S. TI Soil Water Holding Capacity Mitigates Downside Risk and Volatility in US Rainfed Maize: Time to Invest in Soil Organic Matter? SO PLOS ONE LA English DT Article ID CLIMATE-CHANGE; CONSERVATION AGRICULTURE; FOOD SECURITY; CROP YIELDS; PRODUCTIVITY; CARBON; VARIABILITY; ADAPTATION; STABILITY; INCREASE AB Yield stability is fundamental to global food security in the face of climate change, and better strategies are needed for buffering crop yields against increased weather variability. Regional-scale analyses of yield stability can support robust inferences about buffering strategies for widely-grown staple crops, but have not been accomplished. We present a novel analytical approach, synthesizing 2000-2014 data on weather and soil factors to quantify their impact on county-level maize yield stability in four US states that vary widely in these factors (Illinois, Michigan, Minnesota and Pennsylvania). Yield stability is quantified as both 'downside risk' (minimum yield potential, MYP) and 'volatility' (temporal yield variability). We show that excessive heat and drought decreased mean yields and yield stability, while higher precipitation increased stability. Soil water holding capacity strongly affected yield volatility in all four states, either directly (Minnesota and Pennsylvania) or indirectly, via its effects on MYP (Illinois and Michigan). We infer that factors contributing to soil water holding capacity can help buffer maize yields against variable weather. Given that soil water holding capacity responds (within limits) to agronomic management, our analysis highlights broadly relevant management strategies for buffering crop yields against climate variability, and informs region-specific strategies. C1 [Williams, Alwyn; Jordan, Nicholas R.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. [Hunter, Mitchell C.; Kammerer, Melanie; Mortensen, David A.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Kane, Daniel A.; Snapp, Sieglinde] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Smith, Richard G.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Davis, AS (reprint author), USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM asdavis1@illinois.edu OI Hunter, Mitchell/0000-0002-4562-7806; Kammerer Allen, Melanie/0000-0002-0678-9087 FU Agriculture and Food Research Initiative of the United States Department of Agriculture, National Institute for Food and Agriculture [2011-67003-30343] FX This work was supported by Agriculture and Food Research Initiative of the United States Department of Agriculture, National Institute for Food and Agriculture (grant number 2011-67003-30343; https://nifa.usda.gov/program/agriculture-and-food-research-initiative-a fri). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 34 TC 1 Z9 1 U1 5 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 25 PY 2016 VL 11 IS 8 AR e0160974 DI 10.1371/journal.pone.0160974 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DU5NN UT WOS:000382258600022 PM 27560666 ER PT J AU Anton, BP Harhay, GP Smith, TPL Blom, J Roberts, RJ AF Anton, Brian P. Harhay, Gregory P. Smith, Timothy P. L. Blom, Jochen Roberts, Richard J. TI Comparative Methylome Analysis of the Occasional Ruminant Respiratory Pathogen Bibersteinia trehalosi SO PLOS ONE LA English DT Article ID ANALYSIS REVEALS; RESTRICTION; METHYLATION; GENOMICS; METHYLTRANSFERASES; REARRANGEMENTS; SPECIFICITIES; TRANSCRIPTION; ADAPTATION; ANNOTATION AB We examined and compared both the methylomes and the modification-related gene content of four sequenced strains of Bibersteinia trehalosi isolated from the nasopharyngeal tracts of Nebraska cattle with symptoms of bovine respiratory disease complex. The methylation patterns and the encoded DNA methyltransferase (MTase) gene sets were different between each strain, with the only common pattern being that of Dam (GATC). Among the observed patterns were three novel motifs attributable to Type I restriction-modification systems. In some cases the differences in methylation patterns corresponded to the gain or loss of MTase genes, or to recombination at target recognition domains that resulted in changes of enzyme specificity. However, in other cases the differences could be attributed to differential expression of the same MTase gene across strains. The most obvious regulatory mechanism responsible for these differences was slipped strand mispairing within short sequence repeat regions. The combined action of these evolutionary forces allows for alteration of different parts of the methylome at different time scales. We hypothesize that pleiotropic transcriptional modulation resulting from the observed methylomic changes may be involved with the switch between the commensal and pathogenic states of this common member of ruminant microflora. C1 [Anton, Brian P.; Roberts, Richard J.] New England Biolabs Inc, Ipswich, MA 01938 USA. [Harhay, Gregory P.; Smith, Timothy P. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. [Blom, Jochen] Univ Giessen, Bioinformat & Syst Biol, Giessen, Germany. RP Anton, BP (reprint author), New England Biolabs Inc, Ipswich, MA 01938 USA.; Harhay, GP (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. EM anton@neb.com; gregory.harhay@ars.usda.gov FU Small Business Innovation Research Program (NIGMS) of the National Institutes of Health [R44GM105125]; USDA, ARS [5438-32000-029-00D, 5438-31320-012-00D]; BMBF [FKZ 031A533]; New England Biolabs FX This project was supported by the Small Business Innovation Research Program (NIGMS) of the National Institutes of Health under award R44GM105125 to RJR. Funding for this research was provided by the USDA, ARS appropriated projects 5438-32000-029-00D (GPH) and 5438-31320-012-00D (TPLS). The EDGAR platform is financially supported by the BMBF grant FKZ 031A533 within the de. NBI network. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. BPA and RJR are employed by New England Biolabs, a company that sells restriction enzymes, methyltransferases, and other enzymes. New England Biolabs provided support in the form of salaries for BPA and RJR and purchase of research materials, but did not play any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section. NR 49 TC 0 Z9 0 U1 7 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 24 PY 2016 VL 11 IS 8 AR e0161499 DI 10.1371/journal.pone.0161499 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DU5NI UT WOS:000382258100047 PM 27556252 ER PT J AU Butcher, BG Chakravarthy, S D'Amico, K Stoos, KB Filiatrault, MJ AF Butcher, Bronwyn G. Chakravarthy, Suma D'Amico, Katherine Stoos, Kari Brossard Filiatrault, Melanie J. TI Disruption of the carA gene in Pseudomonas syringae results in reduced fitness and alters motility SO BMC MICROBIOLOGY LA English DT Article DE Pseudomonas syringae pv tomato; CarAB; P32; Virulence; Swarming; Biofilm formation ID PV. TOMATO DC3000; RNA-ENCODING GENES; CITRI SUBSP CITRI; ERWINIA-AMYLOVORA; ESCHERICHIA-COLI; CARBAMOYLPHOSPHATE SYNTHETASE; LISTERIA-MONOCYTOGENES; TRANSCRIPTOME ANALYSIS; EXPRESSION TECHNOLOGY; BIOFILM FORMATION AB Background: Pseudomonas syringae infects diverse plant species and is widely used in the study of effector function and the molecular basis of disease. Although the relationship between bacterial metabolism, nutrient acquisition and virulence has attracted increasing attention in bacterial pathology, there is limited knowledge regarding these studies in Pseudomonas syringae. The aim of this study was to investigate the function of the carA gene and the small RNA P32, and characterize the regulation of these transcripts. Results: Disruption of the carA gene (Delta carA) which encodes the predicted small chain of carbamoylphosphate synthetase, resulted in arginine and pyrimidine auxotrophy in Pseudomonas syringae pv. tomato DC3000. Complementation with the wild type carA gene was able to restore growth to wild-type levels in minimal medium. Deletion of the small RNA P32, which resides immediately upstream of carA, did not result in arginine or pyrimidine auxotrophy. The expression of carA was influenced by the concentrations of both arginine and uracil in the medium. When tested for pathogenicity, Delta carA showed reduced fitness in tomato as well as Arabidopsis when compared to the wild-type strain. In contrast, mutation of the region encoding P32 had minimal effect in planta Delta carA also exhibited reduced motility and increased biofilm formation, whereas disruption of P32 had no impact on motility or biofilm formation. Conclusions: Our data show that carA plays an important role in providing arginine and uracil for growth of the bacteria and also influences other factors that are potentially important for growth and survival during infection. Although we find that the small RNA P32 and carA are co-transcribed, P32 does not play a role in the phenotypes that carA is required for, such as motility, cell attachment, and virulence. Additionally, our data suggests that pyrimidines may be limited in the apoplastic space of the plant host tomato. C1 [Butcher, Bronwyn G.; Chakravarthy, Suma; D'Amico, Katherine; Filiatrault, Melanie J.] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [D'Amico, Katherine; Filiatrault, Melanie J.] ARS, USDA, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. [Stoos, Kari Brossard] Ithaca Coll, Sch Hlth Sci & Human Performance, Dept Hlth Promot & Phys Educ, Ithaca, NY 14850 USA. [Butcher, Bronwyn G.] Cornell Univ, Cornell Lab Ornithol, 159 Sapsucker Woods Rd, Ithaca, NY USA. RP Filiatrault, MJ (reprint author), Cornell Univ, Sch Integrat Plant Sci, Sect Plant Pathol & Plant Microbe Biol, Ithaca, NY USA.; Filiatrault, MJ (reprint author), ARS, USDA, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. EM Melanie.filiatrault@ars.usda.gov FU USDA-ARS CRIS project [8062-2100-035-00D] FX This work was supported by the USDA-ARS CRIS project 8062-2100-035-00D, "Pseudomonas Systems Biology". NR 76 TC 1 Z9 1 U1 6 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD AUG 24 PY 2016 VL 16 AR 194 DI 10.1186/s12866-016-0819-z PG 16 WC Microbiology SC Microbiology GA DW1ST UT WOS:000383423600002 PM 27558694 ER PT J AU Zhu, HL Luo, W Ciesielski, PN Fang, ZQ Zhu, JY Henriksson, G Himmel, ME Hu, LB AF Zhu, Hongli Luo, Wei Ciesielski, Peter N. Fang, Zhiqiang Zhu, J. Y. Henriksson, Gunnar Himmel, Michael E. Hu, Liangbing TI Wood-Derived Materials for Green Electronics, Biological Devices, and Energy Applications SO CHEMICAL REVIEWS LA English DT Review ID TEMPO-MEDIATED OXIDATION; LITHIUM-ION BATTERIES; REGENERATED CELLULOSE FILMS; ENHANCED RAMAN-SCATTERING; PLANT-CELL WALL; HIGH-PERFORMANCE SUPERCAPACITORS; OPTICALLY TRANSPARENT WOOD; CHIRAL NEMATIC SUSPENSIONS; NAOH/UREA AQUEOUS-SOLUTION; PAPER-BASED MICROFLUIDICS AB With the arising of global climate change and resource shortage, in recent years, increased attention has been paid to environmentally friendly materials. Trees are sustainable and renewable materials, which give us shelter and oxygen and remove carbon dioxide from the atmosphere. Trees are a primary resource that human society depends upon every day, for example, homes, heating, furniture, and aircraft. Wood from trees gives us paper, cardboard, and medical supplies, thus impacting our homes, school, work, and play. All of the above-mentioned applications have been well developed over the past thousands of years. However, trees and wood have much more to offer us as advanced materials, impacting emerging high-tech fields, such as bioengineering, flexible electronics, and clean energy. Wood naturally has a hierarchical structure, composed of well-oriented microfibers and tracheids for water, ion, and oxygen transportation during metabolism. At higher magnification, the walls of fiber cells have an interesting morphology-a distinctly mesoporous structure. Moreover, the walls of fiber cells are composed of thousands of fibers (or macrofibrils) oriented in a similar angle. Nanofibrils and nanocrystals can be further liberated from macrofibrils by mechanical, chemical, and enzymatic methods. The obtained nanocellulose has unique optical, mechanical, and barrier properties and is an excellent candidate for chemical modification and reconfiguration. Wood is naturally a composite material, comprised of cellulose, hemicellulose, and lignin. Wood is sustainable, earth abundant, strong, biodegradable, biocompatible, and chemically accessible for modification; more importantly, multiscale natural fibers from wood have unique optical properties applicable to different kinds of optoelectronics and photonic devices. Today, the materials derived from wood are ready to be explored for applications in new technology areas, such as electronics, biomedical devices, and energy. The goal of this study is to review the fundamental structures and chemistries of wood and wood-derived materials, which are essential for a wide range of existing and new enabling technologies. The scope of the review covers multiscale materials and assemblies of cellulose, hemicellulose, and lignin as well as other biomaterials derived from wood, in regard to their major emerging applications. Structure properties application relationships will be investigated in detail. Understanding the fundamental properties of these structures is crucial for designing and manufacturing products for emerging applications. Today, a more holistic understanding of the interplay between the structure, chemistry, and performance of wood and wood-derived materials is advancing historical applications of these materials. This new level of understanding also enables a myriad of new and exciting applications, which motivate this review. There are excellent reviews already on the classical topic of woody materials, and some recent reviews also cover new understanding of these materials as well as potential applications. This review will focus on the uniqueness of woody materials for three critical applications: green electronics, biological devices, and energy storage and bioenergy. C1 [Zhu, Hongli; Luo, Wei; Fang, Zhiqiang; Hu, Liangbing] Univ Maryland, Dept Mat Sci & Engn, College Pk, MD 20742 USA. [Zhu, Hongli] Northeastern Univ, Dept Mech & Ind Engn, Boston, MA 02115 USA. [Ciesielski, Peter N.; Himmel, Michael E.] Natl Renewable Energy Lab, Biosci Ctr, 15013 Denver West Pkwy, Golden, CO 80401 USA. [Zhu, J. Y.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Henriksson, Gunnar] KTH, Royal Inst Technol, Dept Fiber & Polymer Technol, Div Wood Chem & Pulp Technol, Stockholm, Sweden. RP Zhu, HL; Hu, LB (reprint author), Univ Maryland, Dept Mat Sci & Engn, College Pk, MD 20742 USA.; Zhu, HL (reprint author), Northeastern Univ, Dept Mech & Ind Engn, Boston, MA 02115 USA. EM h.zhu@neu.edu; binghu@umd.edu RI Luo, Wei/E-1582-2011; Hu, Liangbing/N-6660-2013; OI Luo, Wei/0000-0002-4019-4634; Fang, Zhiqiang/0000-0002-0844-7507 FU DOD (Air Force of Scientific Research) Young Investigator Program [FA95501310143]; 3M Non-Tenured Faculty; NSF-CBET Grant [1335979]; Nanostructures for Electrical Energy Storage (NEES), an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Basic Energy Sciences [DESC0001160]; National Renewable Energy Laboratory's Laboratory Directed Research and Development (LDRD) Program; Northeastern University FX L.H. acknowledges support from the DOD (Air Force of Scientific Research) Young Investigator Program (FA95501310143) and 3M Non-Tenured Faculty and NSF-CBET Grant 1335979. This work also was supported as part of the Nanostructures for Electrical Energy Storage (NEES), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences under Award DESC0001160. Sections 2.1 and 6.2 were written with support from the National Renewable Energy Laboratory's Laboratory Directed Research and Development (LDRD) Program (MEH and PNC). H.Z. acknowledges the startup support from Northeastern University. We also acknowledge Mr. Jiaqi Dai from the University of Maryland for the help on figures. NR 519 TC 10 Z9 10 U1 253 U2 254 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0009-2665 EI 1520-6890 J9 CHEM REV JI Chem. Rev. PD AUG 24 PY 2016 VL 116 IS 16 BP 9305 EP 9374 DI 10.1021/acs.chemrev.6b00225 PG 70 WC Chemistry, Multidisciplinary SC Chemistry GA DU4JO UT WOS:000382179100005 PM 27459699 ER PT J AU Lee, BH Rose, DR Lin, AHM Quezada-Calvillo, R Nichols, BL Hamaker, BR AF Lee, Byung-Hoo Rose, David R. Lin, Amy Hui-Mei Quezada-Calvillo, Roberto Nichols, Buford L. Hamaker, Bruce R. TI Contribution of the Individual Small Intestinal alpha-Glucosidases to Digestion of Unusual alpha-Linked Glycemic Disaccharides SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE alpha-glucosidases; carbohydrate digestion; disaccharides; glycemic; slowly digestible carbohydrates ID MALTASE-GLUCOAMYLASE ACTIVITY; SUCRASE-ISOMALTASE; STARCH DIGESTION; SUBSTRATE BRAKE; SUCROSE; ISOMALTULOSE; SPECIFICITY; HYDROLYSIS; PROTEIN; INTOLERANCE AB The mammalian mucosal alpha-glucosidase complexes, maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI), have two catalytic subunits (N- and C-termini). Concurrent with the desire to modulate glycemic response, there has been a focus on di/oligosaccharides with unusual alpha-linkages that are digested to glucose slowly by these enzymes. Here, we look at disaccharides with various possible alpha-linkages and their hydrolysis. Hydrolytic properties of the maltose and sucrose isomers were determined using rat intestinal and individual recombinant alpha-glucosidases. The individual alpha-glucosidases had moderate to low hydrolytic activities on all alpha-linked disaccharides, except trehalose. Maltase (N-terminal MGAM) showed a higher ability to digest alpha-1,2 and alpha-1,3 disaccharides, as well as alpha-1,4, making it the most versatile in alpha-hydrolytic activity. These findings apply to the development of new glycemic oligosaccharides based on unusual alpha-linkages for extended glycemic response. It also emphasizes that mammalian mucosal alpha-glticosidases must be used in in-vitro assessment of digestion of such carbohydrates. C1 [Lee, Byung-Hoo] Gachon Univ, Coll BioNano Technol, Dept Food Sci & Biotechnol, Songnam 13120, Gyeonggi Do, South Korea. [Lee, Byung-Hoo; Lin, Amy Hui-Mei; Hamaker, Bruce R.] Purdue Univ, Dept Food Sci, Whistler Ctr Carbohydrate Res, Smith Hall, W Lafayette, IN 47907 USA. [Rose, David R.] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada. [Lin, Amy Hui-Mei] Univ Idaho, Bi State Sch Food Sci, Moscow, ID 83844 USA. [Lin, Amy Hui-Mei] Washington State Univ, Moscow, ID 83844 USA. [Quezada-Calvillo, Roberto] Univ Autonoma San Luis Potosi, Dept Chem, San Luis Potosi 78360, Mexico. [Nichols, Buford L.] Baylor Coll Med, USDA, ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Nichols, Buford L.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. [Hamaker, Bruce R.] Sejong Univ, Dept Food Sci & Technol, Seoul 05006, South Korea. RP Hamaker, BR (reprint author), Purdue Univ, Dept Food Sci, Whistler Ctr Carbohydrate Res, Smith Hall, W Lafayette, IN 47907 USA.; Hamaker, BR (reprint author), Sejong Univ, Dept Food Sci & Technol, Seoul 05006, South Korea. EM hamakerb@purdue.edu FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [NRF-2015R1C1A1A02036467]; U.S. Department of Agriculture (USDA) Agriculture and Food Research Initiative [08-555-03-18793]; Whistler Center for Carbohydrate Research at Purdue University, West Lafayette, IN, USA; Canadian Institutes for Health Research; USDA, Agricultural Research Service [58-6250-1-003]; Baylor College of Medicine; Natural Science and Engineering Research Council FX This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A02036467) (B.-H.L.). We are thankful for support from the U.S. Department of Agriculture (USDA) Agriculture and Food Research Initiative competitive grant program, no. 08-555-03-18793 (B.R.H.), the Whistler Center for Carbohydrate Research at Purdue University, West Lafayette, IN, USA, and the Canadian Institutes for Health Research and Natural Science and Engineering Research Council (D.R.R). This work was also supported in part by federal funds from the USDA, Agricultural Research Service, under Cooperative Agreement 58-6250-1-003 with Baylor College of Medicine (B.L.N., R.Q-C.). NR 43 TC 1 Z9 1 U1 8 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 24 PY 2016 VL 64 IS 33 BP 6487 EP 6494 DI 10.1021/acs.jafc.6b01816 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DU4JN UT WOS:000382179000012 PM 27480812 ER PT J AU Chu, TX Chen, RZ Landivar, JA Maeda, MM Yang, CH Starek, MJ AF Chu, Tianxing Chen, Ruizhi Landivar, Juan A. Maeda, Murilo M. Yang, Chenghai Starek, Michael J. TI Cotton growth modeling and assessment using unmanned aircraft system visual-band imagery SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE unmanned aircraft system; cotton yield; plant growth; regression; point cloud; orthomosaics ID PRECISION AGRICULTURE; AERIAL VEHICLE; VEGETATION INDEXES; AIRBORNE LIDAR; UAV IMAGERY; WAVE-FORMS; BIOMASS; CROP; PARAMETERS; MANAGEMENT AB This paper explores the potential of using unmanned aircraft system (UAS)-based visible-band images to assess cotton growth. By applying the structure-from-motion algorithm, the cotton plant height (ph) and canopy cover (cc) information were retrieved from the point cloud-based digital surface models (DSMs) and orthomosaic images. Both UAS-based ph and cc follow a sigmoid growth pattern as confirmed by ground-based studies. By applying an empirical model that converts the cotton ph to cc, the estimated cc shows strong correlation (R-2 = 0.990) with the observed cc. An attempt for modeling cotton yield was carried out using the ph and cc information obtained on June 26, 2015, the date when sigmoid growth curves for both ph and cc tended to decline in slope. In a cross-validation test, the correlation between the ground-measured yield and the estimated equivalent derived from the ph and/or cc was compared. Generally, combining ph and cc, the performance of the yield estimation is most comparable against the observed yield. On the other hand, the observed yield and cc-based estimation produce the second strongest correlation, regardless of the complexity of the models. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. C1 [Chu, Tianxing; Starek, Michael J.] Texas A&M Univ Corpus Christi, Conrad Blucher Inst Surveying & Sci, 6300 Ocean Dr, Corpus Christi, TX 78412 USA. [Chen, Ruizhi] Wuhan Univ, State Key Lab Informat Engn Surveying Mapping & R, 129 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China. [Landivar, Juan A.; Maeda, Murilo M.] Texas A&M AgriLife Res & Extens Ctr, 10345 TX-44, Corpus Christi, TX 78406 USA. [Yang, Chenghai] USDA ARS, 3103 F&B Rd, College Stn, TX 77845 USA. RP Chen, RZ (reprint author), Wuhan Univ, State Key Lab Informat Engn Surveying Mapping & R, 129 Luoyu Rd, Wuhan 430079, Hubei, Peoples R China. EM ruizhi.chen@whu.edu.cn FU Cotton Incorporated Project [15-669TX]; National Science Foundation [1429518] FX This work was partially funded by the Cotton Incorporated Project (No. 15-669TX) and the National Science Foundation Project (No. 1429518). NR 43 TC 0 Z9 0 U1 3 U2 3 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD AUG 23 PY 2016 VL 10 AR 036018 DI 10.1117/1.JRS.10.036018 PG 17 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA EE6MQ UT WOS:000389725800001 ER PT J AU Banuelos, GS Arroyo, IS Dangi, SR Zambrano, MC AF Banuelos, Gary S. Arroyo, Irvin S. Dangi, Sadikshya R. Zambrano, Maria C. TI Continued Selenium Biofortification of Carrots and Broccoli Grown in Soils Once Amended with Se-enriched S. pinnata SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE selenium; biofortification; Stanleya pinnata; carrots; broccoli ID MICROBIAL COMMUNITY STRUCTURE; HYPERACCUMULATOR STANLEYA-PINNATA; DRAINAGE SEDIMENTS; FOLIAR APPLICATION; MINERAL ELEMENTS; SODIUM SELENATE; HUMAN HEALTH; ACCUMULATION; BIOMASS; SPECIATION AB Selenium (Se) biofortification has been practiced in Se-deficient regions throughout the world primarily by adding inorganic sources of Se to the soil. Considering the use of adding organic sources of Se could be useful as an alternative Se amendment for the production of Se-biofortified food crops. In this multi-year micro-plot study, we investigate growing carrots and broccoli in soils that had been previously amended with Se-enriched Stanleya pinnata Pursh (Britton) three and 4 years prior to planting one and two, respectively. Results showed that total and extractable Se concentrations in soils (0-30 cm) were 1.65 mg kg(-1) and 88 mu g L-1, and 0.92 mg kg(-1) and 48.6 mu g L-1 at the beginning of the growing season for planting one and two, respectively. After each respective growing season, total Se concentrations in the broccoli florets and carrots ranged from 6.99 to 7.83 mg kg(-1) and 3.15 to 6.25 mg kg(-1) in planting one and two, respectively. In broccoli and carrot plant tissues, SeMet (selenomethionine) was the predominant selenoamino acid identified in Se aqueous extracts. In postharvest soils from planting one, phospholipid fatty acid (PLFA) analyses showed that amending the soil with S. pinnata exerted no effect on the microbial biomass, AMF (arbuscular mycorrhizal fungi), actinomycetes and Gram-positive and bacterial PLFA at both 0-5 and 0-30 cm, respectively, 3 years later. Successfully producing Se-enriched broccoli and carrots 3 and 4 years later after amending soil with Se-enriched S. pinnata clearly demonstrates its potential source as an organic Se enriched fertilizer for Se-deficient regions. C1 [Banuelos, Gary S.; Arroyo, Irvin S.; Dangi, Sadikshya R.; Zambrano, Maria C.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, Parlier, CA 93648 USA. RP Banuelos, GS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, Water Management Res Unit, Parlier, CA 93648 USA. EM gary.banuelos@ars.usda.gov FU CSU Fresno Agricultural Research Initiative FX Funding for this work was provided by CSU Fresno Agricultural Research Initiative. NR 47 TC 0 Z9 0 U1 20 U2 22 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD AUG 23 PY 2016 VL 7 AR 1251 DI 10.3389/fpls.2016.01251 PG 11 WC Plant Sciences SC Plant Sciences GA DT8JB UT WOS:000381735200001 PM 27602038 ER PT J AU Carim, KJ Christianson, KR McKelvey, KM Pate, WM Silver, DB Johnson, BM Galloway, BT Young, MK Schwartz, MK AF Carim, Kellie J. Christianson, Kyle R. McKelvey, Kevin M. Pate, William M. Silver, Douglas B. Johnson, Brett M. Galloway, Bill T. Young, Michael K. Schwartz, Michael K. TI Environmental DNA Marker Development with Sparse Biological Information: A Case Study on Opossum Shrimp (Mysis diluviana) SO PLOS ONE LA English DT Article ID DROPLET DIGITAL PCR; NORTH-AMERICAN; DIVERSITY; PHYLOGENY; CRUSTACEA; BARCODES AB The spread of Mysis diluviana, a small glacial relict crustacean, outside its native range has led to unintended shifts in the composition of native fish communities throughout western North America. As a result, biologists seek accurate methods of determining the presence of M. diluviana, especially at low densities or during the initial stages of an invasion. Environmental DNA (eDNA) provides one solution for detecting M. diluviana, but building eDNA markers that are both sensitive and species-specific is challenging when the distribution and taxonomy of closely related non-target taxa are poorly understood, published genetic data are sparse, and tissue samples are difficult to obtain. To address these issues, we developed a pair of independent eDNA markers to increase the likelihood of a positive detection of M. diluviana when present and reduce the probability of false positive detections from closely related non-target species. Because tissue samples of closely-related and possibly sympatric, non-target taxa could not be obtained, we used synthetic DNA sequences of closely related non-target species to test the specificity of eDNA markers. Both eDNA markers yielded positive detections from five waterbodies where M. diluviana was known to be present, and no detections in five others where this species was thought to be absent. Daytime samples from varying depths in one waterbody occupied by M. diluviana demonstrated that samples near the lake bottom produced 5 to more than 300 times as many eDNA copies as samples taken at other depths, but all samples tested positive regardless of depth. C1 [Carim, Kellie J.; McKelvey, Kevin M.; Young, Michael K.; Schwartz, Michael K.] US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, 800 E Beckwith Ave, Missoula, MT USA. [Christianson, Kyle R.; Pate, William M.; Silver, Douglas B.; Johnson, Brett M.; Galloway, Bill T.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, 1474 Campus Delivery, Ft Collins, CO 80523 USA. RP Carim, KJ (reprint author), US Forest Serv, Natl Genom Ctr Wildlife & Fish Conservat, Rocky Mt Res Stn, 800 E Beckwith Ave, Missoula, MT USA. EM kelliejcarim@fs.fed.us NR 34 TC 1 Z9 1 U1 23 U2 24 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 23 PY 2016 VL 11 IS 8 AR e0161664 DI 10.1371/journal.pone.0161664 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT8TR UT WOS:000381768800063 PM 27551919 ER PT J AU Restaino, CM Peterson, DL Littell, J AF Restaino, Christina M. Peterson, David L. Littell, Jeremy TI Increased water deficit decreases Douglas fir growth throughout western US forests SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE actual evapotranspiration; dendrochronology; Douglas fir; drought; vapor pressure deficit ID VAPOR-PRESSURE DEFICIT; TREE MORTALITY; UNITED-STATES; CLIMATE-CHANGE; VEGETATION DISTRIBUTION; STOMATAL SENSITIVITY; DROUGHT; TEMPERATURE; RESPONSES; MODEL AB Changes in tree growth rates can affect tree mortality and forest feedbacks to the global carbon cycle. As air temperature increases, evaporative demand also increases, increasing effective drought in forest ecosystems. Using a spatially comprehensive network of Douglas fir (Pseudotsuga menziesii) chronologies from 122 locations that represent distinct climate environments in the western United States, we show that increased temperature decreases growth via vapor pressure deficit (VPD) across all latitudes. Using an ensemble of global circulation models, we project an increase in both the mean VPD associated with the lowest growth extremes and the probability of exceeding these VPD values. As temperature continues to increase in future decades, we can expect deficit-related stress to increase and consequently Douglas fir growth to decrease throughout its US range. C1 [Restaino, Christina M.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Peterson, David L.] US Forest Serv, Pacific Northwest Res Stn, Seattle, WA 98103 USA. [Littell, Jeremy] Alaska Climate Sci Ctr, Dept Interior, Anchorage, AK 99508 USA. RP Restaino, CM (reprint author), Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. EM cmrestaino@ucdavis.edu FU US Department of Energy Office of Science; National Science Foundation Graduate Research Fellowship Program Grant [DGE-0718124]; US Geological Survey Global Change Research Program; US Forest Service Pacific Northwest Research Station; US Department of the Interior Alaska Climate Science Center FX We thank R. Norheim for cartographic assistance; G. Mauger for providing climatic data; N. Povak and M. Kennedy for analytical consultation; Nathan Stephenson and Don McKenzie for preliminary reviews of this manuscript; the modeling groups, the Program for Climate Model Diagnosis and Intercomparison, and the WCRP Working Group on Coupled Modelling for making available the WCRP CMIP3 multimodel dataset, which is supported by the US Department of Energy Office of Science. Funding for this work was provided by National Science Foundation Graduate Research Fellowship Program Grant DGE-0718124, the US Geological Survey Global Change Research Program, the US Forest Service Pacific Northwest Research Station, and the US Department of the Interior Alaska Climate Science Center. This paper is a contribution of the Western Mountain Initiative. NR 38 TC 2 Z9 2 U1 18 U2 21 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD AUG 23 PY 2016 VL 113 IS 34 BP 9557 EP 9562 DI 10.1073/pnas.1602384113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT9ZK UT WOS:000381860800055 PM 27503880 ER PT J AU Idrissi, O Udupa, SM De Keyser, E Mcgee, RJ Coyne, CJ Saha, GC Muehlbauer, FJ Van Damme, P De Riek, J AF Idrissi, Omar Udupa, Sripada M. De Keyser, Ellen McGee, Rebecca J. Coyne, Clarice J. Saha, Gopesh C. Muehlbauer, Fred J. Van Damme, Patrick De Riek, Jan TI Identification of Quantitative Trait Loci Controlling Root and Shoot Traits Associated with Drought Tolerance in a Lentil (Lens culinaris Medik.) Recombinant Inbred Line Population SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE lentil; ecophysiology; drought tolerance; breeding; plant; QTL; marker-assisted selection ID GENETIC-LINKAGE MAP; CICER-ARIETINUM L.; ABIOTIC STRESSES; AFLP MARKERS; RESISTANCE; CONSTRUCTION; ARCHITECTURE; ADAPTATION; BRASSICA; SATIVUM AB Drought is one of the major abiotic stresses limiting lentil productivity in rainfed production systems. Specific rooting patterns can be associated with drought avoidance mechanisms that can be used in lentil breeding programs. In all, 252 co-dominant and dominant markers were used for Quantitative Trait Loci (QTL) analysis on 132 lentil recombinant inbred lines based on greenhouse experiments for root and shoot traits during two seasons under progressive drought-stressed conditions. Eighteen QTLs controlling a total of 14 root and shoot traits were identified. A OIL-hotspot genomic region related to a number of root and shoot characteristics associated with drought tolerance such as dry root biomass, root surface area, lateral root number, dry shoot biomass and shoot length was identified. Interestingly, a OIL (ORSratio(IX_230)) related to root shoot ratio, an important trait for drought avoidance, explaining the highest phenotypic variance of 27.6 and 28.9% for the two consecutive seasons, respectively, was detected. This QTL was closed to the co-dominant SNP marker TP6337 and also flanked by the two SNP TP518 and TP1280. An important QTL (OLRNIII-g864) related to lateral root number was found close to TP3371 and flanked by TP5093 and TP6072 SNP markers. Also, a QTL (QSRL(IV_61.63)) associated with specific root length was identified close to TP1873 and flanked by F7XEM6b SRAP marker and TP1035 SNP marker. These two QTLs were detected in both seasons. Our results could be used for marker-assisted selection in lentil breeding programs targeting root and shoot characteristics conferring drought avoidance as an efficient alternative to slow and labor-intensive conventional breeding methods. C1 [Idrissi, Omar; Van Damme, Patrick] Univ Ghent, Dept Plant Prod, Fac Biosci Engn, Ghent, Belgium. [Idrissi, Omar] Inst Natl Rech Agron Maroc INRA, Ctr Reg Settat, Settat, Morocco. [Udupa, Sripada M.] INRA, Int Ctr Agr Res Dry Areas, Morocco Cooperat Res Project, Rabat, Morocco. [De Keyser, Ellen; De Riek, Jan] Inst Agr & Fisheries Res ILVO, Appl Genet & Breeding, Plant Sci Unit, Melle, Belgium. [McGee, Rebecca J.] ARS, USDA, Grain Legume Genet & Physiol Res, Pullman, WA USA. [Coyne, Clarice J.; Muehlbauer, Fred J.] Washington State Univ, USDA, ARS, Western Reg Plant Introduct, Pullman, WA 99164 USA. [Saha, Gopesh C.] Brotherton Seed Co, Washington, DC USA. [Van Damme, Patrick] Czech Univ Life Sciences, Fac Trop AgriSci, Prague, Czech Republic. RP Idrissi, O (reprint author), Univ Ghent, Dept Plant Prod, Fac Biosci Engn, Ghent, Belgium.; Idrissi, O (reprint author), Inst Natl Rech Agron Maroc INRA, Ctr Reg Settat, Settat, Morocco. EM Omar.Idrissi@UGent.be FU OCP-Foundation-INRA-ICARDA-IAV Hassan II project on India-Morocco Food Legume Initiative FX Of and SU thank OCP-Foundation-INRA-ICARDA-IAV Hassan II project on India-Morocco Food Legume Initiative for the support. NR 61 TC 0 Z9 0 U1 20 U2 20 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD AUG 23 PY 2016 VL 7 AR 1174 DI 10.3389/fpls.2016.01174 PG 11 WC Plant Sciences SC Plant Sciences GA DT8IU UT WOS:000381734400001 PM 27602034 ER PT J AU Njiojob, CN Bozell, JJ Long, BK Elder, T Key, RE Hartwig, WT AF Njiojob, Costyl N. Bozell, Joseph J. Long, Brian K. Elder, Thomas Key, Rebecca E. Hartwig, William T. TI Enantioselective Syntheses of Lignin Models: An Efficient Synthesis of -O-4 Dimers and Trimers by Using the Evans Chiral Auxiliary SO CHEMISTRY-A EUROPEAN JOURNAL LA English DT Article DE aldol reaction; asymmetric synthesis; biomass; enantioselectivity; natural products ID BETA-ARYL ETHER; SP STRAIN SYK-6; BOND-CLEAVAGE; ABSOLUTE-CONFIGURATION; ASYMMETRIC-SYNTHESIS; GUAIACYL ETHER; CONVERSION; OXIDATION; LIGNOCELLULOSE; IDENTIFICATION AB We describe an efficient five-step, enantioselective synthesis of (R,R)- and (S,S)-lignin dimer models possessing a -O-4 linkage, by using the Evans chiral aldol reaction as a key step. Mitsunobu inversion of the (R,R)- or (S,S)-isomers generates the corresponding (R,S)- and (S,R)-diastereomers. We further extend this approach to the enantioselective synthesis of a lignin trimer model. These lignin models are synthesized with excellent ee (>99%) and high overall yields. The lignin dimer models can be scaled up to provide multigram quantities that are not attainable by using previous methodologies. These lignin models will be useful in degradation studies probing the selectivity of enzymatic, microbial, and chemical processes that deconstruct lignin. C1 [Njiojob, Costyl N.; Bozell, Joseph J.; Key, Rebecca E.; Hartwig, William T.] Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN 37996 USA. [Long, Brian K.] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA. [Elder, Thomas] US Forest Serv, Southern Res Stn, USDA, Auburn, AL 36849 USA. RP Bozell, JJ (reprint author), Univ Tennessee, Ctr Renewable Carbon, Knoxville, TN 37996 USA. EM jbozell@utk.edu FU Center for Direct Catalytic Conversion of Biomass to Biofuels, an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Basic Energy Sciences [DE-SC0000997]; University of Tennessee Institute for a Secure and Sustainable Environment (ISSE); Alabama Supercomputer Authority FX This work was supported as part of the Center for Direct Catalytic Conversion of Biomass to Biofuels, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences under Award # DE-SC0000997, and through the University of Tennessee Institute for a Secure and Sustainable Environment (ISSE). The authors are grateful for supercomputer time provided by the Texas Advanced Computing Center at the University of Texas at Austin through MCB-09159. This work was also made possible in part by a grant of high performance computing resources and technical support from the Alabama Supercomputer Authority. NR 62 TC 0 Z9 0 U1 17 U2 17 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 0947-6539 EI 1521-3765 J9 CHEM-EUR J JI Chem.-Eur. J. PD AUG 22 PY 2016 VL 22 IS 35 BP 12506 EP 12517 DI 10.1002/chem.201601592 PG 12 WC Chemistry, Multidisciplinary SC Chemistry GA DV4DX UT WOS:000382876500038 PM 27459234 ER PT J AU Kugadas, A Lamont, EA Bannantine, JP Shoyama, FM Brenner, E Janagama, HK Sreevatsan, S AF Kugadas, Abirami Lamont, Elise A. Bannantine, John P. Shoyama, Fernanda M. Brenner, Evan Janagama, Harish K. Sreevatsan, Srinand TI A Mycobacterium avium subsp paratuberculosis Predicted Serine Protease Is Associated with Acid Stress and Intraphagosomal Survival SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE Mycobacterium avium subsp paratuberculosis; phagosome; intrabacterial pH; serine protease; Johne's disease; macrophage; acid ID GENE-EXPRESSION; PHAGOSOME MATURATION; ESCHERICHIA-COLI; PATHOGENIC MYCOBACTERIA; SSP PARATUBERCULOSIS; TUBERCULOSIS WHIB3; INTRABACTERIAL PH; J774 MACROPHAGES; OXIDATIVE STRESS; INTRACELLULAR PH AB The ability to maintain intra-cellular pH is crucial for bacteria and other microbes to survive in diverse environments, particularly those that undergo fluctuations in pH. Mechanisms of acid resistance remain poorly understood in mycobacteria. Although, studies investigating acid stress in M. tuberculosis are gaining traction, few center on Mycobacterium avium subsp. paratuberculosis (MAP), the etiological agent of chronic enteritis in ruminants. We identified a MAP acid stress response network involved in macrophage infection. The central node of this network was MAP0403, a predicted serine protease that shared an 86% amino acid identity with MarP in M. tuberculosis. Previous studies confirmed MarP as a serine protease integral to maintaining intra-bacterial pH and survival in acid in vitro and in vivo. We show that MAP0403 is upregulated in infected macrophages and MAC-T cells that coincided with phagosome acidification. Treatment of mammalian cells with bafilomcyin A1, a potent inhibitor of phagosomal vATPases, diminished MAP0403 transcription. MAP0403 expression was also noted in acidic medium. A surrogate host, M. smegmatis mc(2) 155, was designed to express MAP0403 and when exposed to either macrophages or in vitro acid stress had increased bacterial cell viability, which corresponds to maintenance of intra-bacterial pH in acidic (pH = 5) conditions, compared to the parent strain. These data suggest that MAP0403 may be the equivalent of MarP in MAP. Future studies confirming MAP0403 as a serine protease and exploring its structure and possible substrates are warranted. C1 [Kugadas, Abirami] Univ Minnesota, Brigham & Womens Hosp, Div Infect Dis, Boston, MA USA. [Lamont, Elise A.; Sreevatsan, Srinand] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. [Bannantine, John P.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. [Shoyama, Fernanda M.; Brenner, Evan; Sreevatsan, Srinand] Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA. [Janagama, Harish K.] Inst Environm Hlth, Lake Forest Pk, WA USA. RP Sreevatsan, S (reprint author), Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA.; Sreevatsan, S (reprint author), Univ Minnesota, Dept Vet Populat Med, St Paul, MN 55108 USA. EM sreev001@umn.edu OI Sreevatsan, Srinand/0000-0002-5162-2403 FU University of Minnesota College of Veterinary Medicine Agriculture Research Station [1802-11646-AES0062027] FX This study was supported by the University of Minnesota College of Veterinary Medicine Agriculture Research Station (1802-11646-AES0062027) grant awarded to SS. NR 60 TC 0 Z9 0 U1 2 U2 3 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD AUG 22 PY 2016 VL 6 AR 85 DI 10.3389/fcimb.2016.00085 PG 11 WC Immunology; Microbiology SC Immunology; Microbiology GA DT7LP UT WOS:000381668600001 PM 27597934 ER PT J AU Rachels, DH Stow, DA O'Leary, JF Johnson, HD Riggan, PJ AF Rachels, Diane H. Stow, Douglas A. O'Leary, John F. Johnson, Harry D. Riggan, Philip J. TI Chaparral recovery following a major fire with variable burn conditions SO INTERNATIONAL JOURNAL OF REMOTE SENSING LA English DT Article ID MEDITERRANEAN-CLIMATE SHRUBLANDS; SANTA-ANA WINDS; SOUTHERN-CALIFORNIA; POSTFIRE RECOVERY; VEGETATION CHANGE; SHORT-INTERVAL; USA; SUCCESSION; IMAGERY; ACCRA AB Wildfires are a common occurrence in California shrublands, maintaining ecosystem functions with the regeneration of key shrub species. The Cedar Fire of 2003 in southern California was unique in that a portion of it burned with wildfire accelerated by dry, strong northeasterly Santa Ana winds that later subsided, while the remaining area burned under an onshore, westerly wind of lower velocity and higher humidity. These nearby areas, having similar terrain, fuel type, and environments, burned under these different conditions. Our goal is to understand the connection between vegetation response to extreme fire events by analysing life-form regrowth in chaparral from the Santa Ana wind driven, Santa Ana backing, and non-Santa Ana fire types. Study sites representing these three fire conditions were based on fire progression maps generated from Moderate Resolution Imaging Spectroradiometer (MODIS) hotspot data. Shrub cover before and six years after the fire were mapped based on a spatial contextual classifier applied to colour infrared orthoimagery, and analysed per slope aspect and angle, elevation, and fire characteristic categories to isolate shrub regrowth patterns. Six years after the fire, shrub cover in the Santa Ana wind driven site was substantially lower than in the other two sites. Such differences in shrub cover at the landscape scale may have resulted from different wind speed, direction, and humidity during the fire, coupled with terrain differences on wildfire behaviour and different rates of recovery associated primarily with moisture availability to plants. The information gathered from this study can help land managers assess shrub regrowth and possibility of vegetation type change after extreme fire events in southern California shrubland ecosystems. C1 [Rachels, Diane H.; Stow, Douglas A.; O'Leary, John F.; Johnson, Harry D.] San Diego State Univ, Dept Geog, 5500 Campanile Dr, San Diego, CA 92182 USA. [Riggan, Philip J.] USDA Forest Serv, Pacific Southwest Res Stn, Riverside, CA USA. RP Stow, DA (reprint author), San Diego State Univ, Dept Geog, 5500 Campanile Dr, San Diego, CA 92182 USA. EM stow@mail.sdsu.edu FU United States Forest Service [10-JV-11279702-101 / G00008499] FX This work was supported by the United States Forest Service [Grant Number 10-JV-11279702-101 / G00008499]. NR 33 TC 1 Z9 1 U1 71 U2 71 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 0143-1161 EI 1366-5901 J9 INT J REMOTE SENS JI Int. J. Remote Sens. PD AUG 20 PY 2016 VL 37 IS 16 BP 3836 EP 3857 DI 10.1080/01431161.2016.1204029 PG 22 WC Remote Sensing; Imaging Science & Photographic Technology SC Remote Sensing; Imaging Science & Photographic Technology GA DS8QT UT WOS:000381048600015 ER PT J AU Yue, YY Han, JQ Han, GP French, AD Qi, YD Wu, QL AF Yue, Yiying Han, Jingquan Han, Guangping French, Alfred D. Qi, Yadong Wu, Qinglin TI Cellulose nanofibers reinforced sodium alginate-polyvinyl alcohol hydrogels: Core-shell structure formation and property characterization SO CARBOHYDRATE POLYMERS LA English DT Article DE Core-shell structure; Hydrogel; Interpenetrating polymer network; Nanoparticles; Absorption ID NANOCOMPOSITE HYDROGEL; ENERGYCANE BAGASSE; THERMAL-PROPERTIES; RELEASE BEHAVIOR; NETWORK HYDROGEL; AQUEOUS-SOLUTION; CELL; NANOPARTICLES; ADSORPTION; MEMBRANES AB Core-shell structured hydrogels consisting of a flexible interpenetrating polymer network (IPN) core and a rigid semi-IPN shell were prepared through chemical crosslinking of polyvinyl alcohol (PVA) and sodium alginate (SA) with Ca2+ and glutaraldehyde. Short cellulose nanofibers (CNFs) extracted from energycane bagasse were incorporated in the hydrogel. The shell was micro-porous and the core was macro-porous. The hydrogels could be used in multiple adsorption-desorption cycles for dyes, and the maximum methyl blue adsorption capacity had a 10% increase after incorporating CNFs. The homogeneous distribution of CNFs in PVA-SA matrix generated additional hydrogen bonds among the polymer molecular chains, resulting in enhanced density, viscoelasticity, and mechanical strength for the hydrogel. Specifically, the compressive strength of the hydrogel reached 79.5 kPa, 3.2 times higher than that of the neat hydrogel. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yue, Yiying; Wu, Qinglin] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Han, Jingquan] Nanjing Forestry Univ, Coll Mat Sci & Engn, Nanjing 210037, Jiangsu, Peoples R China. [Han, Guangping] Northeast Forestry Univ, Minist Educ, Key Lab Biobased Mat Sci & Technol, Harbin 150040, Peoples R China. [French, Alfred D.] USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Qi, Yadong] Southern Univ & A&M Coll, Southern Univ Agr Ctr, Baton Rouge, LA 70813 USA. RP Wu, QL (reprint author), Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. EM QWu@agcenter.lsu.edu FU School of Renewable Natural Resources, LSU AgCenter through the USDA National Institute of Food and Agriculture McIntire Stennis project [1000017] FX This work is supported by the School of Renewable Natural Resources, LSU AgCenter through the USDA National Institute of Food and Agriculture McIntire Stennis project [1000017]. NR 47 TC 3 Z9 3 U1 41 U2 115 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 EI 1879-1344 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD AUG 20 PY 2016 VL 147 BP 155 EP 164 DI 10.1016/j.carbpol.2016.04.005 PG 10 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA DM6GR UT WOS:000376449300020 PM 27178920 ER PT J AU Sun, TT Wiersema, J Yang, Y AF Sun, Tiantian Wiersema, John Yang, Yong TI Correcting the type designations of Caryodaphnopsis baviensis and C. laotica (Lauraceae) SO PHYTOTAXA LA English DT Editorial Material DE Caryodaphnopsis; Lauraceae; typification C1 [Sun, Tiantian; Yang, Yong] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, 20 Nanxincun, Beijing, Peoples R China. [Wiersema, John] ARS, USDA, Natl Germplasm Resources Lab, BARC West, Bldg 003, Beltsville, MD 20705 USA. [Sun, Tiantian] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China. RP Sun, TT (reprint author), Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, 20 Nanxincun, Beijing, Peoples R China. EM ephedra@ibcas.ac.cn NR 11 TC 0 Z9 0 U1 1 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD AUG 19 PY 2016 VL 270 IS 3 BP 227 EP 230 DI 10.11646/phytotaxa.270.3.8 PG 4 WC Plant Sciences SC Plant Sciences GA DU2JO UT WOS:000382037400008 ER PT J AU Chandran, AKN Yoo, YH Cao, PJ Sharma, R Sharma, M Dardick, C Ronald, PC Jung, KH AF Chandran, Anil Kumar Nalini Yoo, Yo-Han Cao, Peijian Sharma, Rita Sharma, Manoj Dardick, Christopher Ronald, Pamela C. Jung, Ki-Hong TI Updated Rice Kinase Database RKD 2.0: enabling transcriptome and functional analysis of rice kinase genes SO RICE LA English DT Article DE Rice kinase database; Phylogenomics; Functional redundancy; Mutant analysis; Meta-analysis; Pearson correlation coefficient; Transcriptome ID PHYLOGENOMIC DATABASE; EXPRESSION; GENOME; IDENTIFICATION; RESOURCE AB Protein kinases catalyze the transfer of a phosphate moiety from a phosphate donor to the substrate molecule, thus playing critical roles in cell signaling and metabolism. Although plant genomes contain more than 1000 genes that encode kinases, knowledge is limited about the function of each of these kinases. A major obstacle that hinders progress towards kinase characterization is functional redundancy. To address this challenge, we previously developed the rice kinase database (RKD) that integrated omics-scale data within a phylogenetics context. An updated version of rice kinase database (RKD) that contains metadata derived from NCBI GEO expression datasets has been developed. RKD 2.0 facilitates in-depth transcriptomic analyses of kinase-encoding genes in diverse rice tissues and in response to biotic and abiotic stresses and hormone treatments. We identified 261 kinases specifically expressed in particular tissues, 130 that are significantly up- regulated in response to biotic stress, 296 in response to abiotic stress, and 260 in response to hormones. Based on this update and Pearson correlation coefficient (PCC) analysis, we estimated that 19 out of 26 genes characterized through loss-of-function studies confer dominant functions. These were selected because they either had paralogous members with PCC values of < 0.5 or had no paralog. Compared with the previous version of RKD, RKD 2.0 enables more effective estimations of functional redundancy or dominance because it uses comprehensive expression profiles rather than individual profiles. The integrated analysis of RKD with PCC establishes a single platform for researchers to select rice kinases for functional analyses. C1 [Chandran, Anil Kumar Nalini; Yoo, Yo-Han; Jung, Ki-Hong] Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea. [Chandran, Anil Kumar Nalini; Yoo, Yo-Han; Jung, Ki-Hong] Kyung Hee Univ, Crop Biotech Inst, Yongin 446701, South Korea. [Cao, Peijian] Zhengzhou Tobacco Res Inst, China Tobacco Gene Res Ctr, Zhengzhou 450001, Peoples R China. [Sharma, Rita] Jawaharlal Nehru Univ, Sch Computat & Integrat Sci, New Delhi 110067, India. [Sharma, Manoj] Jawaharlal Nehru Univ, Sch Biotechnol, New Delhi 110067, India. [Dardick, Christopher] ARS, Appalachian Fruit Res Stn, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25442 USA. [Ronald, Pamela C.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Ronald, Pamela C.] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Ronald, Pamela C.] Joint Bioenergy Inst, Emeryville, CA 95616 USA. RP Jung, KH (reprint author), Kyung Hee Univ, Grad Sch Biotechnol, Yongin 446701, South Korea.; Jung, KH (reprint author), Kyung Hee Univ, Crop Biotech Inst, Yongin 446701, South Korea.; Ronald, PC (reprint author), Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA.; Ronald, PC (reprint author), Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. EM pcronald@ucdavis.edu; khjung2010@khu.ac.kr FU Cooperative Research Program for Agriculture Science & Technology Development [PJ01182602]; Rural Development Administration, Republic of Korea; DOE Joint BioEnergy Institute by the U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]; Lawrence Berkeley National Laboratory; U. S. Department of Energy FX This work was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development ( Project No. PJ01182602)", Rural Development Administration, Republic of Korea, and was part of the DOE Joint BioEnergy Institute (http://www.jbei.org) supported by the U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the U. S. Department of Energy. The United States Government retains and the publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for United States Government purposes. NR 22 TC 0 Z9 0 U1 3 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-8425 EI 1939-8433 J9 RICE JI Rice PD AUG 19 PY 2016 VL 9 AR 40 DI 10.1186/s12284-016-0106-5 PG 16 WC Agronomy SC Agriculture GA DU1XQ UT WOS:000382004400001 PM 27540739 ER PT J AU Tabima, JF Everhart, SE Larsen, MM Weisberg, AJ Kamvar, ZN Tancos, MA Smart, CD Chang, JH Grunwald, NJ AF Tabima, Javier F. Everhart, Sydney E. Larsen, Meredith M. Weisberg, Alexandra J. Kamvar, Zhian N. Tancos, Matthew A. Smart, Christine D. Chang, Jeff H. Grunwald, Niklaus J. TI Microbe-ID: an open source toolbox for microbial genotyping and species identification SO PEERJ LA English DT Article DE Taxonomy; Identification; Molecular diagnostics; Phytophthora; Genotyping; Pathogen ID SUDDEN OAK DEATH; INTERNAL TRANSCRIBED SPACER; PHYTOPHTHORA-RAMORUM; R-PACKAGE; MICROSATELLITE MARKERS; NUCLEOTIDE-SEQUENCES; GENETIC-DISTANCE; UNITED-STATES; POPULATIONS; PATHOGEN AB Development of tools to identify species, genotypes, or novel strains of invasive organisms is critical for monitoring emergence and implementing rapid response measures. Molecular markers, although critical to identifying species or genotypes, require bioinformatic tools for analysis. However, user-friendly analytical tools for fast identification are not readily available. To address this need, we created a web-based set of applications called Microbe-ID that allow for customizing a toolbox for rapid species identification and strain genotyping using any genetic markers of choice. Two components of Microbe-ID, named Sequence-ID and Genotype-ID, implement species and genotype identification, respectively. Sequence-ID allows identification of species by using BLAST to query sequences for any locus of interest against a custom reference sequence database. Genotype-ID allows placement of an unknown multilocus marker in either a minimum spanning network or dendrogram with bootstrap support from a user-created reference database. Microbe-ID can be used for identification of any organism based on nucleotide sequences or any molecular marker type and several examples are provided. We created a public website for demonstration purposes called Microbe-ID (www.microbe-id.org ) and provided a working implementation for the genus Phytophthora (www.phytophthora-id.org ). In Phytophthora-ID, the Sequence-ID application allows identification based on ITS or cox spacer sequences. Genotype-ID groups individuals into clonal lineages based on simple sequence repeat (SSR) markers for the two invasive plant pathogen species P. infestans and P. ramorum. All code is open source and available on github and CRAN. Instructions for installation and use are provided at https://github.com/grunwaldlab/Microbe-ID. C1 [Tabima, Javier F.; Everhart, Sydney E.; Weisberg, Alexandra J.; Kamvar, Zhian N.; Chang, Jeff H.; Grunwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Larsen, Meredith M.; Grunwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Tancos, Matthew A.; Smart, Christine D.; Grunwald, Niklaus J.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA. [Chang, Jeff H.; Grunwald, Niklaus J.] Oregon State Univ, Mol & Cellular Biol Grad Program, Corvallis, OR 97331 USA. [Chang, Jeff H.; Grunwald, Niklaus J.] Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. [Everhart, Sydney E.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Grunwald, NJ (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Grunwald, NJ (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA.; Grunwald, NJ (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Geneva, NY 14456 USA.; Grunwald, NJ (reprint author), Oregon State Univ, Mol & Cellular Biol Grad Program, Corvallis, OR 97331 USA.; Grunwald, NJ (reprint author), Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. EM nik.grunwald@ars.usda.gov OI Tabima, Javier/0000-0002-3603-2691 FU US Department of Agriculture (USDA) Agricultural Research Service Grant [5358-22000-039-00D]; USDA National Institute of Food and Agriculture (NIFA) Grant [2011-68004-30154]; USDA ARS Floriculture Nursery Research Initiative; USDA NIFA Grant [2014-51181-22384]; USDA NIFA [2012-67012-19844] FX This research is supported in part by the US Department of Agriculture (USDA) Agricultural Research Service Grant 5358-22000-039-00D (NJG), USDA National Institute of Food and Agriculture (NIFA) Grant 2011-68004-30154 (NJG), the USDA ARS Floriculture Nursery Research Initiative (NJG), USDA NIFA Grant 2014-51181-22384 (JHC and NJG), and USDA NIFA 2012-67012-19844 (SEE). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 58 TC 0 Z9 0 U1 3 U2 3 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD AUG 18 PY 2016 VL 4 AR UNSP e2279 DI 10.7717/peerj.2279 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DV8RB UT WOS:000383203100003 PM 27602267 ER PT J AU Augustinos, AA Rajamohan, A Kyritsis, GA Zacharopoulou, A ul Haq, I Targovska, A Caceres, C Bourtzis, K Abd-Alla, AMM AF Augustinos, Antonios A. Rajamohan, Arun Kyritsis, Georgios A. Zacharopoulou, Antigone ul Haq, Ihsan Targovska, Asya Caceres, Carlos Bourtzis, Kostas Abd-Alla, Adly M. M. TI Cryopreservation of Embryos of the Mediterranean Fruit Fly Ceratitis capitata Vienna 8 Genetic Sexing Strain SO PLOS ONE LA English DT Article ID DIPTERA-TEPHRITIDAE; MUSCIDAE EMBRYOS; STERILE; CALLIPHORIDAE; SUPPRESSION; FLIES; DOMESTICATION; LEPIDOPTERA; ERADICATION; GUATEMALA AB The Mediterranean fruit fly, Ceratitis capitata, is one of the most serious pests of fruit crops world-wide. During the last decades, area-wide pest management (AW-IPM) approaches with a sterile insect technique (SIT) component have been used to control populations of this pest in an effective and environment-friendly manner. The development of genetic sexing strains (GSS), such as the Vienna 8 strain, has been played a major role in increasing the efficacy and reducing the cost of SIT programs. However, mass rearing, extensive inbreeding, possible bottleneck phenomena and hitch-hiking effects might pose major risks for deterioration and loss of important genetic characteristics of domesticated insect. In the present study, we present a modified procedure to cryopreserve the embryos of the medfly Vienna 8 GSS based on vitrification and used this strain as insect model to assess the impact of the cryopreservation process on the genetic structure of the cryopreserved insects. Forty-eight hours old embryos, incubated at 24 degrees C, were found to be the most suitable developmental stage for cryopreservation treatment for high production of acceptable hatch rate (38%). Our data suggest the absence of any negative impact of the cryopreservation process on egg hatch rate, pupation rates, adult emergence rates and stability of the temperature sensitive lethal (tsl) character on two established cryopreserved lines (flies emerged from cryopreserved embryos), named V8-118 and V8-228. Taken together, our study provides an optimized procedure to cryopreserve the medfly Vienna 8 GSS and documents the absence of any negative impact on the genetic structure and quality of the strain. Benefits and sceneries for utilization of this technology to support operational SIT projects are discussed in this paper. C1 [Augustinos, Antonios A.; Kyritsis, Georgios A.; ul Haq, Ihsan; Targovska, Asya; Caceres, Carlos; Bourtzis, Kostas; Abd-Alla, Adly M. M.] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. [Rajamohan, Arun] USDA, Insect Genet & Biochem Unit, Biosci Res Lab, Fargo, ND 58105 USA. [Zacharopoulou, Antigone] Univ Patras, Dept Biol, Patras, Greece. RP Abd-Alla, AMM (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Vienna, Austria. EM a.m.m.abd-alla@iaea.org NR 54 TC 0 Z9 0 U1 6 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 18 PY 2016 VL 11 IS 8 AR e0160232 DI 10.1371/journal.pone.0160232 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT6ES UT WOS:000381577000015 ER PT J AU Casas, E Cai, G Kuehn, LA Register, KB McDaneld, TG Neill, JD AF Casas, Eduardo Cai, Guohong Kuehn, Larry A. Register, Karen B. McDaneld, Tara G. Neill, John D. TI Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle SO PLOS ONE LA English DT Article ID RESPIRATORY-DISEASE; HEME OXYGENASE-1; HOST-DEFENSE; EXPRESSION; BIOMARKERS; TRANSCRIPTOME; INFECTION; PROFILES; MUSCLE; VIRUS AB The objective of this study was to identify microRNAs associated with a serum antibody response to Mycoplasma bovis in beef cattle. Serum from sixteen beef calves was collected at three points: in summer after calves were born, in fall at weaning, and in the following spring. All sera collected in the summer were ELISA-negative for anti-M. bovis. By the fall, eight animals were seropositive for IgG (positive group), while eight remained negative (negative group). By spring, all animals in both groups were seropositive. MicroRNAs were extracted from sera and sequenced on the Illumina HiSeq next-generation sequencer. A total of 1,374,697 sequences mapped to microRNAs in the bovine genome. Of these, 82% of the sequences corresponded to 27 microRNAs, each represented by a minimum of 10,000 sequences. There was a statistically significant interaction between ELISA response and season for bta-miR-24-3p (P = 0.0268). All sera collected at the initial summer had a similar number of copies of this microRNA (P = 0.773). In the fall, the positive group had an increased number of copies when compared to the negative group (P = 0.021), and this grew more significant by the following spring (P = 0.0001). There were 21 microRNAs associated (P< 0.05) with season. These microRNAs could be evaluated further as candidates to potentially improve productivity in cattle. The microRNAs bta-let-7b, bta-miR- 24-3p, bta-miR- 92a, and bta-miR-423-5p, were significatly associated with ELISA status (P< 0.05). These microRNAs have been recognized as playing a role in the host defense against bacteria in humans, mice, and dairy cattle. Further studies are needed to establish if these microRNAs could be used as diagnostic marker or indicator of exposure, or whether intervention strategies could be developed as an alternative to antibiotics for controlling disease due to M. bovis. C1 [Casas, Eduardo; Cai, Guohong; Register, Karen B.; Neill, John D.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Kuehn, Larry A.; McDaneld, Tara G.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Casas, E (reprint author), ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM eduardo.casas@ars.usda.gov FU USDA FX This research was conducted at a USDA research facility and all funding was provided through internal USDA research dollars. This project is an intramural project of the USDA/ARS National Animal Disease Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 37 TC 1 Z9 1 U1 7 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 18 PY 2016 VL 11 IS 8 AR e0161651 DI 10.1371/journal.pone.0161651 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT6ES UT WOS:000381577000129 ER PT J AU Chan, AW Hamblin, MT Jannink, JL AF Chan, Ariel W. Hamblin, Martha T. Jannink, Jean-Luc TI Evaluating Imputation Algorithms for Low-Depth Genotyping-By-Sequencing (GBS) Data SO PLOS ONE LA English DT Article ID GENOMIC SELECTION; ASSOCIATION; ACCURACY; MODELS AB Well-powered genomic studies require genome-wide marker coverage across many individuals. For non-model species with few genomic resources, high-throughput sequencing (HTS) methods, such as Genotyping-By-Sequencing (GBS), offer an inexpensive alternative to array-based genotyping. Although affordable, datasets derived from HTS methods suffer from sequencing error, alignment errors, and missing data, all of which introduce noise and uncertainty to variant discovery and genotype calling. Under such circumstances, meaningful analysis of the data is difficult. Our primary interest lies in the issue of how one can accurately infer or impute missing genotypes in HTS-derived datasets. Many of the existing genotype imputation algorithms and software packages were primarily developed by and optimized for the human genetics community, a field where a complete and accurate reference genome has been constructed and SNP arrays have, in large part, been the common genotyping platform. We set out to answer two questions: 1) can we use existing imputation methods developed by the human genetics community to impute missing genotypes in datasets derived from non-human species and 2) are these methods, which were developed and optimized to impute ascertained variants, amenable for imputation of missing genotypes at HTS-derived variants? We selected Beagle v.4, a widely used algorithm within the human genetics community with reportedly high accuracy, to serve as our imputation contender. We performed a series of cross-validation experiments, using GBS data collected from the species Manihot esculenta by the Next Generation (NEXTGEN) Cassava Breeding Project. NEXTGEN currently imputes missing genotypes in their datasets using a LASSO-penalized, linear regression method (denoted 'glmnet'). We selected glmnet to serve as a benchmark imputation method for this reason. We obtained estimates of imputation accuracy by masking a subset of observed genotypes, imputing, and calculating the sample Pearson correlation between observed and imputed genotype dosages at the site and individual level; computation time served as a second metric for comparison. We then set out to examine factors affecting imputation accuracy, such as levels of missing data, read depth, minor allele frequency (MAF), and reference panel composition. C1 [Chan, Ariel W.; Jannink, Jean-Luc] Cornell Univ, Sch Integrat Plant Sci, Sect Plant Breeding & Genet, Ithaca, NY 14850 USA. [Hamblin, Martha T.] Cornell Univ, Inst Genom Div, Ithaca, NY USA. [Jannink, Jean-Luc] ARS, USDA, RW Holley Ctr Agr & Hlth, Ithaca, NY USA. RP Chan, AW (reprint author), Cornell Univ, Sch Integrat Plant Sci, Sect Plant Breeding & Genet, Ithaca, NY 14850 USA. EM ac2278@cornell.edu FU Bill & Melinda Gates Foundation; Department for International Development of the United Kingdom; National Science Foundation FX The Next Generation (NEXTGEN) Cassava Breeding Project is supported by a grant from the Bill & Melinda Gates Foundation and the Department for International Development of the United Kingdom. JLJ and MTH received funding from the Bill & Melinda Gates Foundation and the Department for International Development of the United Kingdom. AWC received funding from the National Science Foundation. The funders had no role in study design, data analysis, decision to publish, or preparation of the manuscript. NR 20 TC 1 Z9 1 U1 6 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 18 PY 2016 VL 11 IS 8 AR e0160733 DI 10.1371/journal.pone.0160733 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT6ES UT WOS:000381577000029 ER PT J AU Dickinson, MB Hutchinson, TF Dietenberger, M Matt, F Peters, MP AF Dickinson, Matthew B. Hutchinson, Todd F. Dietenberger, Mark Matt, Frederick Peters, Matthew P. TI Litter Species Composition and Topographic Effects on Fuels and Modeled Fire Behavior in an Oak-Hickory Forest in the Eastern USA SO PLOS ONE LA English DT Article ID SOUTHERN APPALACHIAN MOUNTAINS; HARDWOOD LEAF-LITTER; DECOMPOSITION DYNAMICS; PLANT FLAMMABILITY; PRESCRIBED FIRES; LIGNIN CONTROL; UNITED-STATES; NORTH-AMERICA; REGENERATION; VEGETATION AB Mesophytic species (esp. Acer rubrum) are increasingly replacing oaks (Quercus spp.) in fire-suppressed, deciduous oak-hickory forests of the eastern US. A pivotal hypothesis is that fuel beds derived from mesophytic litter are less likely than beds derived from oak litter to carry a fire and, if they do, are more likely to burn at lower intensities. Species effects, however, are confounded by topographic gradients that affect overstory composition and fuel bed decomposition. To examine the separate and combined effects of litter species composition and topography on surface fuel beds, we conducted a common garden experiment in oak-hickory forests of the Ohio Hills. Each common garden included beds composed of mostly oak and mostly maple litter, representative of oak-and maple-dominated stands, respectively, and a mixture of the two. Beds were replenished each fall for four years. Common gardens (N = 16) were established at four topographic positions (ridges, benches on south-and northeast-facing slopes, and stream terraces) at each of four sites. Litter source and topographic position had largely independent effects on fuel beds and modeled fire dynamics after four years of development. Loading (kg m(-2)) of the upper litter layer (L), the layer that primarily supports flaming spread, was least in more mesic landscape positions and for maple beds, implying greater decomposition rates for those situations. Bulk density in the L layer (kg m(-3)) was least for oak beds which, along with higher loading, would promote fire spread and fireline intensity. Loading and bulk density of the combined fermentation and humic (FH) layers were least on stream terrace positions but were not related to species. Litter-and FH-layer moistures during a 5-day dry-down period after a rain event were affected by time and topographic effects while litter source effects were not evident. Characteristics of flaming combustion determined with a cone calorimeter pointed to greater fireline intensity for oak fuel beds and unexpected interactions between litter source and topography. A spread index, which synthesizes a suite of fuel bed, particle, and combustion characteristics to indicate spread (vs extinction) potential, was primarily affected by litter source and, secondarily, by the low spread potentials on mesic landscape positions early in the 5-day dry-down period. A similar result was obtained for modeled fireline intensity. Our results suggest that the continuing transition from oaks to mesophytic species in the Ohio Hills will reduce fire spread potentials and fire intensities. C1 [Dickinson, Matthew B.; Hutchinson, Todd F.; Peters, Matthew P.] US Forest Serv, Northern Res Stn, Delaware, OH 43015 USA. [Dietenberger, Mark; Matt, Frederick] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Dickinson, MB (reprint author), US Forest Serv, Northern Res Stn, Delaware, OH 43015 USA. EM mbdickinson@fs.fed.us FU Northern Research Station; National Fire Plan FX This project was funded by the Northern Research Station and National Fire Plan.; The Common Garden experiment could not have been conducted without the coordination and work in the field by the Vinton Furnace State Experimental Forest crew, especially Bill Borovicka, Levi Miller, and David Hosack. Tony Bova helped with dry-down sampling. John Stanovick, Northern Research Station Statistician, provided indispensable assistance with statistical analyses. Antoine Randolph, Mike Stambaugh, Tom Waldrop, and Joe O'Brien and one anonymous reviewer provided helpful reviews of earlier drafts. This project was funded by the Northern Research Station and National Fire Plan. NR 72 TC 0 Z9 0 U1 22 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 18 PY 2016 VL 11 IS 8 AR e0159997 DI 10.1371/journal.pone.0159997 PG 30 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT6ES UT WOS:000381577000012 ER PT J AU Song, B An, LX Han, YJ Gao, HX Ren, HB Zhao, X Wei, XS Krishnan, HB Liu, SS AF Song, Bo An, Lixin Han, Yanjing Gao, Hongxiu Ren, Hongbo Zhao, Xue Wei, Xiaoshuang Krishnan, Hari B. Liu, Shanshan TI Transcriptome Profile of Near-Isogenic Soybean Lines for beta-Conglycinin alpha-Subunit Deficiency during Seed Maturation SO PLOS ONE LA English DT Article ID GLYCINE-MAX L; STORAGE-PROTEIN GENES; 7S GLOBULIN; L MERRILL; RNA-SEQ; EXPRESSION; SUPERFAMILY; INHERITANCE; IDENTIFICATION; ALLERGENS AB Crossing, backcrossing, and molecular marker-assisted background selection produced a soybean (Glycine max) near-isogenic line (cgy-2-NIL) containing the cgy-2 allele, which is responsible for the absence of the allergenic alpha-subunit of beta-conglycinin. To identify alpha-null-related transcriptional changes, the gene expressions of cgy-2-NIL and its recurrent parent DN47 were compared using Illumina high-throughput RNA-sequencing of samples at 25, 35, 50, and 55 days after flowering (DAF). Seeds at 18 DAF served as the control. Comparison of the transcript profiles identified 3,543 differentially expressed genes (DEGs) between the two genotypes, with 2,193 genes downregulated and 1,350 genes upregulated. The largest numbers of DEGs were identified at 55 DAF. The DEGs identified at 25 DAF represented a unique pattern of GO category distributions. KEGG pathway analyses identified 541 altered metabolic pathways in cgy-2-NIL. At 18DAF, 12 DEGs were involved in arginine and proline metabolism. The cgy-2 allele in the homozygous form modified the expression of several Cupin allergen genes. The cgy-2 allele is an alteration of a functional allele that is closely related to soybean protein amino acid quality, and is useful for hypoallergenic soybean breeding programs that aim to improve seed protein quality. C1 [Song, Bo; Han, Yanjing; Gao, Hongxiu; Zhao, Xue; Wei, Xiaoshuang; Liu, Shanshan] Northeast Agr Univ, Chinese Agr Minist, Key Lab Soybean Biol & Breeding Genet, Key Lab Soybean Biol, Harbin 150030, Peoples R China. [Ren, Hongbo] Minist Agr China, Inspect & Testing Ctr Qual Cereals & Their Prod, Harbin 150030, Peoples R China. [Krishnan, Hari B.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO USA. [An, Lixin] Harbin Med Univ, Affiliated Hosp 1, Dept Allergy, Harbin, Peoples R China. RP Liu, SS (reprint author), Northeast Agr Univ, Chinese Agr Minist, Key Lab Soybean Biol & Breeding Genet, Key Lab Soybean Biol, Harbin 150030, Peoples R China. EM ars336699@aliyun.com FU National Natural Science Foundation of China [31071440, 31371650]; Northeast Agricultural University Innovation Foundation [yjscx14042] FX The work was supported by the National Natural Science Foundation of China (31071440 and 31371650) and Northeast Agricultural University Innovation Foundation for Postgraduates (yjscx14042). The study is part of the PhD research of the first author. NR 51 TC 0 Z9 0 U1 6 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 17 PY 2016 VL 11 IS 8 AR e0159723 DI 10.1371/journal.pone.0159723 PG 25 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT4YN UT WOS:000381487600010 ER PT J AU Hernandes, FA Skvarla, MJ Fisher, JR Dowling, APG Ochoa, R Ueckermann, EA Bauchan, GR AF Hernandes, Fabio A. Skvarla, Michael J. Fisher, J. Ray Dowling, Ashley P. G. Ochoa, Ronald Ueckermann, Edward A. Bauchan, Gary R. TI Catalogue of snout mites (Acariformes: Bdellidae) of the world SO ZOOTAXA LA English DT Article DE mite; Eupodina; Bdelloidea; systematics; taxonomy; biodiversity; predatory mites; predators; soil mites ID SMINTHURUS-VIRIDIS COLLEMBOLA; TROPICA ATYEO ACARI; LONGAUDA FOLSOM COLLEMBOLA; BDELLODES-JAPONICUS EHARA; BIOLOGICAL-CONTROL; TROMBIDIFORMES BDELLIDAE; DIFFERENT TEMPERATURES; PROSTIGMATA BDELLIDAE; FUNCTIONAL-RESPONSES; FAMILY BDELLIDAE AB Bdellidae (Trombidiformes: Prostigmata) are moderate to large sized predatory mites that inhabit soil, leaves, leaf litter, and intertidal rocks. They are readily recognized by an elongated, snout-like gnathosoma and by elbowed pedipalps bearing two (one in Monotrichobdella Baker & Balock) long terminal setae. Despite being among the first mites ever described, with species described by Carl Linnaeus, the knowledge about bdellids has never been compiled into a taxonomic catalogue. Here we present a catalogue listing 278 valid species; for each species we include distribution information, taxonomic literature, and type depository institutions. The genus Rigibdella Tseng, 1978 is considered a junior synonym of Cyta von Heyden, 1826, and Bdellodes Oudemans, 1937 is considered a junior synonym of Odontoscirus Tohr, 1913. Illustrated keys to subfamilies and genera are presented, as well as keys to species of each genus. C1 [Hernandes, Fabio A.] Univ Estadual Paulista, Dept Zool, Ave 24-A,1515, BR-13506900 Rio Claro, SP, Brazil. [Skvarla, Michael J.; Fisher, J. Ray; Dowling, Ashley P. G.] Univ Arkansas, Dept Entomol, 319 AGRI Bldg, Fayetteville, AR 72701 USA. [Skvarla, Michael J.] Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. [Skvarla, Michael J.; Ochoa, Ronald] USDA, Systemat Entomol Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Ueckermann, Edward A.] North West Univ, Sch Biol Sci Zool, Potchefstroom Campus, ZA-2520 Potchefstroom, South Africa. [Bauchan, Gary R.] USDA, Electron & Confocal Microscopy Unit, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Hernandes, FA (reprint author), Univ Estadual Paulista, Dept Zool, Ave 24-A,1515, BR-13506900 Rio Claro, SP, Brazil. EM abakashi@gmail.com RI Gesseff, Ednilson/A-3019-2017 NR 335 TC 1 Z9 1 U1 1 U2 6 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD AUG 17 PY 2016 VL 4152 IS 1 BP 1 EP + DI 10.11646/zootaxa.4152.1.1 PG 82 WC Zoology SC Zoology GA DT5EO UT WOS:000381504300001 PM 27615820 ER PT J AU Shellito, PJ Small, EE Colliander, A Bindlish, R Cosh, MH Berg, AA Bosch, DD Caldwell, TG Goodrich, DC McNairn, H Prueger, JH Starks, PJ van der Velde, R Walker, JP AF Shellito, Peter J. Small, Eric E. Colliander, Andreas Bindlish, Rajat Cosh, Michael H. Berg, Aaron A. Bosch, David D. Caldwell, Todd G. Goodrich, David C. McNairn, Heather Prueger, John H. Starks, Patrick J. van der Velde, Rogier Walker, Jeffrey P. TI SMAP soil moisture drying more rapid than observed in situ following rainfall events SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article DE Soil Moisture Active Passive (SMAP); validation; drydown; in situ monitoring ID ATMOSPHERE COUPLING EXPERIMENT; VALIDATION; DYNAMICS; STABILITY; SMOS; PERSISTENCE; GLACE AB We examine soil drying rates by comparing surface soil moisture observations from the NASA Soil Moisture Active Passive (SMAP) mission to those from networks of in situ probes upscaled to SMAP's sensing footprint. SMAP and upscaled in situ probes record different soil drying dynamics after rainfall. We modeled this process by fitting an exponential curve to 63 drydown events: the median SMAP drying timescale is 44% shorter and the magnitude of drying is 35% greater than in situ measurements. We also calculated drying rates between consecutive observations from 193 events. For 6days after rainfall, soil moisture from SMAP dries at twice the rate of in situ measurements. Restricting in situ observations to times of SMAP observations does not change the drying timescale, magnitude, or rate. Therefore, observed differences are likely due to differences in sensing depths: SMAP measures shallower soil moisture than in situ probes, especially after rainfall. C1 [Shellito, Peter J.; Small, Eric E.] Univ Colorado, Dept Geol Sci, Boulder, CO 80309 USA. [Colliander, Andreas] CALTECH, NASA Jet Prop Lab, Pasadena, CA 91125 USA. [Bindlish, Rajat; Cosh, Michael H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Berg, Aaron A.] Univ Guelph, Dept Geog, Guelph, ON, Canada. [Bosch, David D.] USDA ARS, Southeast Watershed Res Lab, Tifton, GA 31793 USA. [Caldwell, Todd G.] Univ Texas Austin, Bur Econ Geol, Jackson Sch Geosci, Austin, TX USA. [Goodrich, David C.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ USA. [McNairn, Heather] Agr & Agri Food Canada, Ottawa, ON, Canada. [Prueger, John H.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Starks, Patrick J.] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. [van der Velde, Rogier] Univ Twente, Fac Geoinformat Sci & Earth Observat ITC, Enschede, Netherlands. [Walker, Jeffrey P.] Monash Univ, Dept Civil Engn, Melbourne, Vic, Australia. RP Shellito, PJ (reprint author), Univ Colorado, Dept Geol Sci, Boulder, CO 80309 USA. EM peter.shellito@colorado.edu RI Caldwell, Todd/H-5129-2011 OI Caldwell, Todd/0000-0003-4068-0648 FU NASA [NNX13AF43G]; Environment Canada from the Canadian Space Agency FX This research was supported by NASA grant NNX13AF43G. SMAP data on the validation grid were provided by the SMAP passive soil moisture team members: R. Bindlish, S. Chan, T. Jackson, P. O'Neill, and E. Njoku. Precipitation data used in this study were acquired as part of the mission of NASA's Earth Science Division and archived and distributed by the Goddard Earth Sciences Data and Information Services Center. Thanks to all who have provided high-quality in situ soil moisture data, including Mark Seyfried and the Reynolds Creek Experimental Watershed; Stan Livingston of the St. Joseph's Experimental Watershed (USDA-Agricultural Research Service); Jose Martinez-Fernandez and the REMEDHUS network; Mahta Moghaddam and the Tonzi Ranch SoilSCAPE project; and Ernesto Lopez-Baeza and the Valencia network. The Kenaston network is supported by Environment Canada from grants from the Canadian Space Agency; Tracy Rowlandson and Erica Tetlock are acknowledged for their work with the network. The data used are listed and provided in the supporting information. NR 43 TC 0 Z9 0 U1 12 U2 12 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD AUG 16 PY 2016 VL 43 IS 15 BP 8068 EP 8075 DI 10.1002/2016GL069946 PG 8 WC Geosciences, Multidisciplinary SC Geology GA DV9VM UT WOS:000383290300033 ER PT J AU Jordan, MA Castaneda, AJ Smiley, PC Gillespie, RB Smith, DR King, KW AF Jordan, Mark A. Castaneda, Abel J. Smiley, Peter C., Jr. Gillespie, Robert B. Smith, Douglas R. King, Kevin W. TI Influence of instream habitat and water chemistry on amphibians in channelized agricultural headwater streams SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Hydrology; Nutrients; Herbicides; Anurans ID EAST CENTRAL INDIANA; FROGS RANA-PIPIENS; SPECIES RICHNESS; MACROINVERTEBRATE COMMUNITIES; AQUATIC COMMUNITIES; MULTIPLE STRESSORS; RIPARIAN HABITATS; SPATIAL LOCATION; DRAINAGE DITCHES; FISH ASSEMBLAGES AB The widespread use of stream channelization and subsurface tile drainage for removing water from agricultural fields has led to the development of numerous channelized agricultural headwater streams within agricultural watersheds of the Midwestern United States, Canada, and Europe. Channelized agricultural headwater streams have been documented to serve as habitat for amphibians, but information on amphibian habitat relationships within these streams is lacking and needed for developing effective conservation strategies. We quantified instream habitat, water chemistry, and sampled amphibians from seven sites in three channelized streams in Cedar Creek, Indiana in 2008 and 2009 and five sites in five channelized streams in Upper Big Walnut Creek, Ohio in 2009. We conducted an indirect gradient analysis involving the use of principal component analysis and generalized linear mixed effect model analysis to determine which variables had the greatest influence on amphibian community and population structure. Overall, amphibian community and population structure was most strongly correlated with water chemistry rather than instream habitat within channelized agricultural headwater streams in Indiana and Ohio. Eleven of 12 amphibian response variables were most strongly correlated with either a water chemistry gradient of nitrate + nitrite and acetochlor, a gradient of total nitrogen and ammonia, or a gradient of simazine and total phosphorus. Only one amphibian response variable was most strongly correlated with an instream habitat gradient of dissolved oxygen and water depth. Our results suggest that conservation strategies that target reductions of nutrients and herbicides will provide the greatest benefits for amphibians within channelized agricultural headwater streams. Published by Elsevier B.V. C1 [Jordan, Mark A.; Castaneda, Abel J.; Gillespie, Robert B.] Indiana Univ Purdue Univ, Dept Biol, Ft Wayne, IN 46805 USA. [Smiley, Peter C., Jr.; King, Kevin W.] ARS, USDA, Soil Drainage Unit, Columbus, OH 43210 USA. [Smith, Douglas R.] ARS, USDA, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Smith, Douglas R.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Jordan, MA (reprint author), Indiana Univ Purdue Univ, Dept Biol, Ft Wayne, IN 46805 USA. EM jordanma@ipfw.edu OI Gillespie, Robert/0000-0003-1216-0733 FU USDA National Soil Erosion Laboratory at Purdue University [106770]; Department of Biology at Indiana University-Purdue University Fort Wayne FX We thank K Aram, S. Hess, B. Judge, and J. Sposito for helping with the collection of amphibians. We also thank the numerous current and past USDA-ARS National Soil Erosion Laboratory, USDA-ARS Soil Drainage Research Unit, and Indiana University-Purdue University Fort Wayne personnel for their assistance with field and laboratory work associated with collection of instream habitat and water chemistry data. Landowner, site, and watershed information were provided by Soil and Water Conservation and NRCS districts in Delaware (Ohio), Morrow (Ohio), Allen (Indiana), Dekalb (Indiana), and Noble (Indiana) Counties. The USDA National Soil Erosion Laboratory at Purdue University (Sponsored Research Grant #106770) and the Department of Biology at Indiana University-Purdue University Fort Wayne provided partial funding for the CC ecology research. N. Fausey and C. Huang provided logistical support for this research effort. We are also grateful to the landowners who provided access to the sites. NR 95 TC 0 Z9 0 U1 22 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD AUG 16 PY 2016 VL 230 BP 87 EP 97 DI 10.1016/j.agee.2016.05.028 PG 11 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DT9PJ UT WOS:000381834500010 ER PT J AU Smart, MD Pettis, JS Euliss, N Spivak, MS AF Smart, Matthew D. Pettis, Jeff S. Euliss, Ned Spivak, Marla S. TI Land use in the Northern Great Plains region of the US influences the survival and productivity of honey bee colonies SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Agriculture; Land use; Apis mellifera; Colony survival; Honey bee; Honey production; Pesticide exposure; Pollen collection ID UNITED-STATES; APIS-MELLIFERA; INFORMED PARTNERSHIP; COLLAPSE DISORDER; NATIONAL-SURVEY; LOSSES; WINTER; HYMENOPTERA; RESISTANCE; COUMAPHOS AB The Northern Great Plains region of the US annually hosts a large portion of commercially managed U.S. honey bee colonies each summer. Changing land use patterns over the last several decades have contributed to declines in the availability of bee forage across the region, and the future sustainability of the region to support honey bee colonies is unclear. We examined the influence of varying land use on the survivorship and productivity of honey bee colonies located in six apiaries within the Northern Great Plains state of North Dakota, an area of intensive agriculture and high density of beekeeping operations. Land use surrounding the apiaries was quantified over three years, 2010-2012, and survival and productivity of honey bee colonies were determined in response to the amount of bee forage land within a 3.2-km radius of each apiary. The area of uncultivated forage land (including pasture, USDA conservation program fields, fallow land, flowering woody plants, grassland, hay land, and roadside ditches) exerted a positive impact on annual apiary survival and honey production. Taxonomic diversity of bee-collected pollen and pesticide residues contained therein varied seasonally among apiaries, but overall were not correlated to large-scale land use patterns or survival and honey production. The predominant flowering plants utilized by honey bee colonies for pollen were volunteer species present in unmanaged (for honey bees), and often ephemeral, lands; thus placing honey bee colonies in a precarious situation for acquiring forage and nutrients over the entire growing season. We discuss the implications for land management, conservation, and beekeeper site selection in the Northern Great Plains to adequately support honey bee colonies and insure long term security for pollinator-dependent crops across the entire country. (C) 2016 Elsevier B.V. All rights reserved. C1 [Smart, Matthew D.; Spivak, Marla S.] Univ Minnesota, Dept Entomol, St Paul, MN USA. [Pettis, Jeff S.] ARS, USDA, Bee Res Lab, Beltsville, MD USA. [Euliss, Ned] US Geol Survey, Northern Prairie Wildlife Res Ctr, 8711 37th St SE, Jamestown, MD 58401 USA. RP Smart, MD (reprint author), US Geol Survey, Northern Prairie Wildlife Res Ctr, 8711 37th St SE, Jamestown, MD 58401 USA. EM msmart@usgs.gov; jeff.pettis@ars.usda.gov; eulissfamilyinnd@gmail.com; spiva001@umn.edu OI Spivak, Marla/0000-0002-6822-3636 FU USDA-NIFA; North Dakota Department of Agriculture FX The authors would like to thank the collaborating beekeeper Zac Browning, USDA technician Nathan Rice, and USGS technicians Jordan Neau and Cali Roth. We would also like to thank Margaret McDermott for assistance with pollen identification. This project was funded by grants from USDA-NIFA and the North Dakota Department of Agriculture. NR 55 TC 3 Z9 3 U1 43 U2 65 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD AUG 16 PY 2016 VL 230 BP 139 EP 149 DI 10.1016/j.agee.2016.05.030 PG 11 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DT9PJ UT WOS:000381834500015 ER PT J AU Diaz-Garcia, L Covarrubias-Pazaran, G Schlautman, B Zalapa, J AF Diaz-Garcia, Luis Covarrubias-Pazaran, Giovanny Schlautman, Brandon Zalapa, Juan TI GiNA, an Efficient and High-Throughput Software for Horticultural Phenotyping SO PLOS ONE LA English DT Article ID IMAGE-ANALYSIS; PHENOMICS; RESPONSES; CRANBERRY; DEFECTS; GROWTH; RICE AB Traditional methods for trait phenotyping have been a bottleneck for research in many crop species due to their intensive labor, high cost, complex implementation, lack of reproducibility and propensity to subjective bias. Recently, multiple high-throughput phenotyping platforms have been developed, but most of them are expensive, species-dependent, complex to use, and available only for major crops. To overcome such limitations, we present the open-source software GiNA, which is a simple and free tool for measuring horticultural traits such as shape-and color-related parameters of fruits, vegetables, and seeds. GiNA is multi-platform software available in both R and MATLAB (R) programming languages and uses conventional images from digital cameras with minimal requirements. It can process up to 11 different horticultural morphological traits such as length, width, two-dimensional area, volume, projected skin, surface area, RGB color, among other parameters. Different validation tests produced highly consistent results under different lighting conditions and camera setups making GiNA a very reliable platform for high-throughput phenotyping. In addition, fivefold cross validation between manually generated and GiNA measurements for length and width in cranberry fruits were 0.97 and 0.92. In addition, the same strategy yielded prediction accuracies above 0.83 for color estimates produced from images of cranberries analyzed with GiNA compared to total anthocyanin content (TAcy) of the same fruits measured with the standard methodology of the industry. Our platform provides a scalable, easy-to-use and affordable tool for massive acquisition of phenotypic data of fruits, seeds, and vegetables. C1 [Diaz-Garcia, Luis; Covarrubias-Pazaran, Giovanny; Schlautman, Brandon; Zalapa, Juan] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Diaz-Garcia, Luis] Inst Nacl Invest Forest & Agr & Pecuarias, Aguascalientes, Mexico. [Zalapa, Juan] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Diaz-Garcia, L; Zalapa, J (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA.; Diaz-Garcia, L (reprint author), Inst Nacl Invest Forest & Agr & Pecuarias, Aguascalientes, Mexico.; Zalapa, J (reprint author), Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. EM diaz.antonio@inifap.gob.mx; jezalapa@wisc.edu FU USDA-ARS [3655-21220-001-00]; WI-DATCP (SCBG Project) [14-002]; National Science Foundation [DBI-1228280]; Ocean Spray Cranberries, Inc.; Wisconsin Cranberry Growers Association; Cranberry Institute; Frank B. Koller Cranberry Fellowship for Graduate Students; Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico) FX This project was supported by USDA-ARS (project no. 3655-21220-001-00 provided to JZ); WI-DATCP (SCBG Project #14-002); National Science Foundation (DBI-1228280); Ocean Spray Cranberries, Inc.; Wisconsin Cranberry Growers Association; Cranberry Institute. BS was supported by the Frank B. Koller Cranberry Fellowship for Graduate Students. GCP and LDG were supported by the Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; J.Z. and B.S. wish to express their gratitude through Mt 10:32. We wish to thank Bill Hatch, Nicole Hansen, and Leah Hyatt from Cranberry Creek Cranberries Inc. for allowing us to harvest the cranberry samples used to validate GiNA. Also, thank Eric Wiesman, Walter Salazar, Yiwen Ge and Sarah Hodapp for their help in taking pictures and processing fruit samples. This project was supported by USDA-ARS (project no. 3655-21220-001-00 provided to J.Z.); WI-DATCP (SCBG Project #14-002); National Science Foundation (DBI-1228280); Ocean Spray Cranberries, Inc.; Wisconsin Cranberry Growers Association; Cranberry Institute. B.S. was supported by the Frank B. Koller Cranberry Fellowship for Graduate Students. GCP and LDG were supported by the Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico). We thank to the anonymous reviewers who helped to enhance the quality of this paper. NR 20 TC 0 Z9 0 U1 4 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 16 PY 2016 VL 11 IS 8 AR e0160439 DI 10.1371/journal.pone.0160439 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT4UN UT WOS:000381476700006 PM 27529547 ER PT J AU Schwarz, RS Moran, NA Evans, JD AF Schwarz, Ryan S. Moran, Nancy A. Evans, Jay D. TI Early gut colonizers shape parasite susceptibility and microbiota composition in honey bee workers SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE cytochrome P450; dysbiosis; microbial interaction; parasitism; vitellogenin ID COLONY COLLAPSE DISORDER; APIS-MELLIFERA WORKERS; BUMBLE BEES; GENETIC DIVERSITY; GEN. NOV.; PARASACCHARIBACTER-APIUM; CYTOCHROME-P450 GENES; PROTOZOAN PARASITES; INTESTINAL PARASITE; HYMENOPTERA-APIDAE AB Microbial symbionts living within animal guts are largely composed of resident bacterial species, forming communities that often provide benefits to the host. Gut microbiomes of adult honey bees (Apis mellifera) include core residents such as the betaproteobacterium Snod-grassella alvi, alongside transient parasites such as the protozoan Lotmaria passim. To test how these species affect microbiome composition and host physiology, we administered S. alvi and/or L. passim inocula to newly emergedworker bees from four genetic backgrounds (G(H)) and reared them in normal (within hives) or stressed (protein-deficient, asocial) conditions. Microbiota acquired by normal bees were abundant but quantitatively differed across treatments, indicating treatment-associated dysbiosis. Pretreatment with S. alvi made normal bees more susceptible to L. passim and altered developmental and detoxification gene expression. Stressed bees were more susceptible to L. passim and were depauperate in core microbiota, yet supplementation with S. alvi did not alter this susceptibility. Microbiomes were generally more variable by G(H) in stressed bees, which also showed opposing and comparatively reduced modulation of gene expression responses to treatments compared with normal bees. These data provide experimental support for a link between altered gut microbiota and increased parasite and pathogen prevalence, as observed from honey bee colony collapse disorder. C1 [Schwarz, Ryan S.; Evans, Jay D.] USDA, Beltsville Agr Res Ctr, Bee Res Lab, Beltsville, MD 20705 USA. [Schwarz, Ryan S.] Ft Lewis Coll, Dept Biol, Durango, CO 81301 USA. [Moran, Nancy A.] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. RP Schwarz, RS (reprint author), USDA, Beltsville Agr Res Ctr, Bee Res Lab, Beltsville, MD 20705 USA.; Schwarz, RS (reprint author), Ft Lewis Coll, Dept Biol, Durango, CO 81301 USA. EM rsschwarz@fortlewis.edu OI Evans, Jay/0000-0002-0036-4651 FU National Science Foundation Grants [1046153, 1415604] FX We thank Kim Hammond, Waldan Kwong, Eli Powell, and Allen Smith for providing S. alvi cultures, culturing advice, and equipment and the anonymous reviewers who improved the manuscript. This work was supported by National Science Foundation Grants 1046153 and 1415604 (to N.A.M. and J.D.E.). NR 80 TC 4 Z9 4 U1 27 U2 36 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD AUG 16 PY 2016 VL 113 IS 33 BP 9345 EP 9350 DI 10.1073/pnas.1606631113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3RS UT WOS:000381399200066 PM 27482088 ER PT J AU Ukuku, DO Geveke, DJ Chau, L Niemira, BA AF Ukuku, Dike O. Geveke, David J. Chau, Lee Niemira, Brendan A. TI Microbial safety and overall quality of cantaloupe fresh-cut pieces prepared from whole fruit after wet steam treatment SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Hot water; Wet steam; Bacterial pathogens; Whole cantaloupe; Fresh-cut cantaloupe ID MINIMALLY PROCESSED FRUITS; CELL-SURFACE CHARGE; ESCHERICHIA-COLI; NATIVE MICROFLORA; HYDROGEN-PEROXIDE; LISTERIA-MONOCYTOGENES; SALMONELLA-TYPHIMURIUM; DISINFECTION; ATTACHMENT; CHLORINE AB Fresh-cut cantaloupes have been associated with outbreaks of Salmonellosis. Minimally processed fresh-cut fruits have a limited shelf life because of deterioration caused by spoilage microflora and physiological processes. The objectives of this study were to use a wet steam process to 1) reduce indigenous spoilage microflora and inoculated populations of Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes on the surface of cantaloupes, and 2) reduce the populations counts in cantaloupe fresh-cut pieces after rind removal and cutting. The average inocula of Salmonella, E. coli O157:H7 and Listeria monocytogenes was 10(7) CFU/ml and the populations recovered on the cantaloupe rind surfaces after inoculation averaged 4.5, 4.8 and 4.1 log CFU/cm(2), respectively. Whole cantaloupes were treated with a wet steam processing unit for 180 s, and the treated melons were stored at 5 degrees C for 29 days. Bacterial populations in fresh-cut pieces prepared from treated and control samples stored at 5 and 10 degrees C for up to 12 days were determined and changes in color (CIE L*, a*, and b*) due to treatments were measured during storage. Presence and growth of aerobic mesophilic bacteria and Salmonella, E. coli 0157:H7 and L monocytogenet were determined in fresh-cut cantaloupe samples. There were no visual signs of physical damage on all treated cantaloupe surfaces immediately after treatments and during storage. All fresh-cut pieces from treated cantaloupes rind surfaces were negative for bacterial pathogens even after an enrichment process. Steam treatment significantly (p < 0.05) changed the color of the fresh-cut pieces. Minimal wet steam treatment of cantaloupes rind surfaces designated for fresh-cut preparation will enhance the microbial safety of fresh-cut pieces, by reducing total bacterial populations. This process holds the potential to significantly reduce the incidence of foodborne illness associated with fresh-cut fruits. (C) Published by Elsevier B.V. C1 [Ukuku, Dike O.; Geveke, David J.; Chau, Lee; Niemira, Brendan A.] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. RP Ukuku, DO (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM dike.ukuku@ars.usda.gov NR 38 TC 0 Z9 0 U1 13 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD AUG 16 PY 2016 VL 231 BP 86 EP 92 DI 10.1016/j.ijfoodmicro.2016.05.019 PG 7 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DQ5XB UT WOS:000379277500012 PM 27240141 ER PT J AU Pomeroy, JW Fang, X Marks, DG AF Pomeroy, John W. Fang, Xing Marks, Danny G. TI The cold rain-on-snow event of June 2013 in the Canadian Rockies characteristics and diagnosis SO HYDROLOGICAL PROCESSES LA English DT Article DE rain-on-snow; flooding; snowmelt; flowpaths; cold regions hydrological model; Canadian Rockies ID INCOMING LONGWAVE RADIATION; MELTING SNOW; MOUNTAIN CATCHMENT; HEADWATER BASIN; ENERGY-BALANCE; FOREST COVER; MASS-BALANCE; MODEL; PRECIPITATION; ACCUMULATION AB The June 2013 flood in the Canadian Rockies featured rain-on-snow (ROS) runoff generation at alpine elevations that contributed to the high streamflows observed during the event. Such a mid-summer ROS event has not been diagnosed in detail, and a diagnosis may help to understand future high discharge-producing hydrometeorological events in mountainous cold regions. The alpine hydrology of the flood was simulated using a physically based model created with the modular cold regions hydrological modelling platform. The event was distinctive in that, although at first, relatively warm rain fell onto existing snowdrifts inducing ROS melt; the rainfall turned to snowfall as the air mass cooled and so increased snowcover and snowpacks in alpine regions, which then melted rapidly from ground heat fluxes in the latter part of the event. Melt rates of existing snowpacks were substantially lower during the ROS than during the relatively sunny periods preceding and following the event as a result of low wind speeds, cloud cover and cool temperatures. However, at the basin scale, melt volumes increased during the event as a result of increased snowcover from the fresh snowfall and consequent large ground heat contributions to melt energy, causing snowmelt to enhance rainfall-runoff by one fifth. Flow pathways also shifted during the event from relatively slow sub-surface flow prior to the flood to an even contribution from sub-surface and fast overland flow during and immediately after the event. This early summer, high precipitation ROS event was distinctive for the impact of decreased solar irradiance in suppressing melt rates, the contribution of ground heat flux to basin scale snowmelt after precipitation turned to snowfall, the transition from slow sub-surface to fast overland flow runoff as the sub-surface storage saturated and streamflow volumes that exceeded precipitation. These distinctions show that summer, mountain ROS events should be considered quite distinct from winter ROS and can be important contributors to catastrophic events. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Pomeroy, John W.; Fang, Xing] Univ Saskatchewan, Ctr Hydrol, Saskatoon, SK, Canada. [Marks, Danny G.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. RP Pomeroy, JW (reprint author), Univ Saskatchewan, Ctr Hydrol, Saskatoon, SK, Canada. EM john.pomeroy@usask.ca OI Fang, Xing/0000-0002-4333-4815 FU Alberta departments of Agriculture and Forestry and Environment and Parks; Natural Sciences and Engineering Research Council of Canada (NSERC); Canadian Foundation for Innovation; NSERC Changing Cold Regions Network; Canada Research Chairs programme; Canada Excellence Research Chair in Water Security; Canadian Foundation for Climate and Atmospheric Sciences; USDA Agricultural Research Service FX The authors wish to acknowledge funding from Alberta departments of Agriculture and Forestry and Environment and Parks, the Natural Sciences and Engineering Research Council of Canada (NSERC) through its Discovery and Research Tools and Instruments Grants, the Canadian Foundation for Innovation, the NSERC Changing Cold Regions Network, Canada Research Chairs programme, the Canada Excellence Research Chair in Water Security, the Canadian Foundation for Climate and Atmospheric Sciences and the USDA Agricultural Research Service and logistical assistance from Nakiska Ski Resort and the Biogeoscience Institute of the University of Calgary. NR 52 TC 2 Z9 2 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD AUG 15 PY 2016 VL 30 IS 17 BP 2899 EP 2914 DI 10.1002/hyp.10905 PG 16 WC Water Resources SC Water Resources GA DV5HK UT WOS:000382957700001 ER PT J AU Niemeyer, RJ Link, TE Seyfried, MS Flerchinger, GN AF Niemeyer, Ryan J. Link, Timothy E. Seyfried, Mark S. Flerchinger, Gerald N. TI Surface water input from snowmelt and rain throughfall in western juniper: potential impacts of climate change and shifts in semi-arid vegetation SO HYDROLOGICAL PROCESSES LA English DT Article DE interception; throughfall; snow; surface water input; western juniper; climate change ID UNITED-STATES; CANOPY INTERCEPTION; DECIDUOUS FOREST; ARCTIC TUNDRA; MODEL; ACCUMULATION; STEMFLOW; BASIN; COMMUNITIES; GENERATION AB In the western USA, shifts from snow to rain precipitation regimes and increases in western juniper cover in shrub-dominated landscapes can alter surface water input via changes in snowmelt and throughfall. To better understand how shifts in both precipitation and semi-arid vegetation cover alter above-ground hydrological processes, we assessed how rain interception differs between snow and rain surface water input; how western juniper alters snowpack dynamics; and how these above-ground processes differ across western juniper, mountain big sagebrush and low sagebrush plant communities. We collected continuous surface water input with four large lysimeters, interspace and below-canopy snow depth data and conducted periodic snow surveys for two consecutive water years (2013 and 2014). The ratio of interspace to below-canopy surface water input was greater for snow relative to rain events, averaging 79.4% and 54.8%, respectively. The greater surface water input ratio for snow is in part due to increased deposition of redistributed snow under the canopy. We simulated above-ground energy and water fluxes in western juniper, low sagebrush and mountain big sagebrush for two 8-year periods under current and projected mid-21st century warmer temperatures with the Simultaneous Heat and Water (SHAW) model. Juniper compared with low and mountain sagebrush reduced surface water input by an average of 138mm or 24% of the total site water budget. Conversely, warming temperatures reduced surface water input by only an average of 14mm across the three vegetation types. The future (warmer) simulations resulted in earlier snow disappearance and surface water input by 51 and 45days, respectively, across juniper, low sagebrush and mountain sagebrush. Information from this study can help land managers in the sagebrush steppe understand how both shifts in climate and semi-arid vegetation will alter fundamental hydrological processes. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Niemeyer, Ryan J.] Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA. [Link, Timothy E.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83843 USA. [Seyfried, Mark S.; Flerchinger, Gerald N.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. RP Niemeyer, RJ (reprint author), Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA. EM rniemeyer07@gmail.com FU National Science Foundation [0903479, 0854553]; United States Geological Survey's Northwest Climate Science Center Doctoral Fellowship FX The authors wish to thank Steve Van Vactor, Mark Murdock, Ben Soderquist and Jim Hoppie for their help with fieldwork. We thank the Reynolds Creek CZO NSF (EAR 1331872) for their field and data support. This work was funded by the National Science Foundation's IGERT (Award 0903479) and CBET (Award 0854553) programmes and the United States Geological Survey's Northwest Climate Science Center Doctoral Fellowship. We also thank John Abatzoglou and the Northwest Knowledge Network for making the Multivariate Adaptive Constructed Analogs and the World Climate Research Programme's Working Group on Coupled Modelling and the climate modelling groups (listed in Table S1) for downscaled projections available. NR 67 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD AUG 15 PY 2016 VL 30 IS 17 BP 3046 EP 3060 DI 10.1002/hyp.10845 PG 15 WC Water Resources SC Water Resources GA DV5HK UT WOS:000382957700011 ER PT J AU Cerqueira-Cezar, CK Pedersen, K Calero-Bernal, R Kwok, OC Villena, I Dubey, JP AF Cerqueira-Cezar, Camila K. Pedersen, Kerri Calero-Bernal, Rafael Kwok, Oliver C. Villena, Isabelle Dubey, Jitender P. TI Seroprevalence of Neospora caninum in feral swine (Sus scrofa) in the United States SO VETERINARY PARASITOLOGY LA English DT Article DE Feral swine (Sus scrofa); Neospora agglutination test (NAT); Seroprevalence; United States ID TOXOPLASMA-GONDII; ANTIBODIES; INFECTION; LATRANS AB The protozoon Neospora caninum is a major cause of abortion in cattle worldwide. Canids (Canis familiaris, Canis latrans, Canis lupus) are definitive hosts whereas many other animal species, including pigs, are intermediate hosts for the parasite. Between 2012 and 2014, serum samples from 1059 feral swine (Sus scrofa) from 29 states of the USA were tested for N. caninum antibodies, using the N. caninum agglutination test (NAT). Of these, 159 (15.0%) feral pigs from 21 states tested positive, with a range of titers of 1:25 (cut-off) (n=153), 1:200 (1), 1:400 (1), 1:800 (3) and 1:3200 (1). Results indicate widespread exposure of feral swine to N. caninum infection across the USA. Published by Elsevier B.V. C1 [Cerqueira-Cezar, Camila K.; Calero-Bernal, Rafael; Kwok, Oliver C.; Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Pedersen, Kerri] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Villena, Isabelle] Univ Reims, EA 3800, Lab Parasitol Mycol, Natl Reference Ctr Toxoplasmosis,Biol Ressource C, 45 Rue Cognacq Jay, F-51092 Reims, France. [Villena, Isabelle] Hop Maison Blanche, 45 Rue Cognacq Jay, F-51092 Reims, France. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. EM Jitender.Dubey@ars.usda.gov NR 12 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD AUG 15 PY 2016 VL 226 BP 35 EP 37 DI 10.1016/j.vetpar.2016.06.023 PG 3 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DU6UT UT WOS:000382350800008 PM 27514880 ER PT J AU Gennari, SM Niemeyer, C Soares, HS Musso, CM Siqueira, GCC Catao-Dias, JL Dias, RA Dubey, JP AF Gennari, Solange M. Niemeyer, Claudia Soares, Herbert S. Musso, Cesar M. Siqueira, Glauber C. C. Catao-Dias, Jose L. Dias, Ricardo A. Dubey, Jitender P. TI Seroprevalence of Toxoplasma gondii in seabirds from Abrolhos Archipelago, Brazil SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Seabirds; Sula spp.; Phaeton spp. ID OOCYSTS; SURVIVAL AB Toxoplasma gondii is a coccidian parasite that infects almost all warm-blooded animals, including birds. Abrolhos is an archipelago of five islands, located in the Atlantic Ocean, 56 nautical kilometers from the south coast of the state of Bahia, northeastern Brazil. Part of this archipelago is a National Marine Park, which is a conservation area protected by the Brazilian government. The objective of this study was to determine the occurrence of T. gondii antibodies in sera of seabird's species Sula spp. and Phaeton spp. from breeding colonies located in the Islands of Santa Barbara and Redonda, Abrolhos's archipelago. Sera were tested by modified agglutination test, first screened at 1:5 dilution (cut-off point) and the positive samples were titrated at a two-fold serial dilution. Serum samples were obtained from 69 birds of four species: Sula dactylatra (23 birds), Sula leucogaster (19 birds), Phaeton aethereus (25 birds) and Phaeton lepturus (2 birds). Antibodies to T. gondii were found in 24 (34.8%) of 69 seabirds with titers that ranged from 5 to 640. Occurrence value in S. dactylatra was 34.8% (8/23), in S. leucogaster was 47.4% (9/19), in P. aethereus was 28% (7/25) and the 2 P. lepturus were negative. This is the first description of T. gondii antibodies in free ranging seabirds of the orders Suliformes and Phaethontiformes. (C) 2016 Elsevier B.V. All rights reserved. C1 [Gennari, Solange M.; Soares, Herbert S.; Dias, Ricardo A.] Univ Sao Paulo, Fac Vet Med FMVZ, Dept Prevent Vet Med & Anim Hlth, Av Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. [Niemeyer, Claudia; Catao-Dias, Jose L.] FMVZ USP, Dept Pathol, Av Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. [Musso, Cesar M.; Siqueira, Glauber C. C.] Avidepa Associacao Vila Velhense Protecao Ambient, Vila Velha, ES, Brazil. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab,BARC East, Bldg 1001, Beltsville, MD 20705 USA. RP Gennari, SM (reprint author), Univ Sao Paulo, Fac Vet Med FMVZ, Dept Prevent Vet Med & Anim Hlth, Av Prof Orlando Marques de Paiva,87,Cidade Univ, BR-05508270 Sao Paulo, SP, Brazil. EM sgennari@usp.br RI Catao-Dias, Jose Luiz/C-4897-2012 OI Catao-Dias, Jose Luiz/0000-0003-2999-3395 FU National Brazilian Research Council (CNPq)CNPq, Brazil; CNPq [141948/2011-6] FX S.M.Gennari and J.L.Catao-Dias are in receipting of a fellowship from National Brazilian Research Council (CNPq)CNPq, Brazil. C.Niemeyer expeditions were sponsored by CNPq (141948/2011-6). We would like to thanks ICMBio, Brazilian Marine and the Abrolhos Marine Reserve. NR 11 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD AUG 15 PY 2016 VL 226 BP 50 EP 52 DI 10.1016/j.vetpar.2016.06.016 PG 3 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DU6UT UT WOS:000382350800011 PM 27514883 ER PT J AU Di Bello, PL Laney, AG Druciarek, T Ho, TE Gergerich, RC Keller, KE Martin, RR Tzanetakis, IE AF Di Bello, Patrick L. Laney, Alma G. Druciarek, Tobiasz Ho, Thien Gergerich, Rose C. Keller, Karen E. Martin, Robert R. Tzanetakis, Ioannis E. TI A novel emaravirus is associated with redbud yellow ringspot disease SO VIRUS RESEARCH LA English DT Article DE Emaravinis; Next generation sequencing; Diversity; Redbud; Legume virus ID FIG-MOSAIC-VIRUS; LEAF-BLOTCH-VIRUS; MULTIPLE SEQUENCE ALIGNMENT; ERIOPHYID MITE; NUCLEOTIDE-SEQUENCE; GENETIC DIVERSITY; RNA SEGMENTS; GENOMIC RNA; PROTEIN; GENERATION AB Yellow ringspot is the only virus-like disease reported in redbud (Cercis spp.) with symptoms including vein clearing, chlorotic ringspots and oak-leaf pattern. A putative new emaravirus was present in all trees displaying typical yellow ringspot symptoms and the name redbud yellow ringspot associated virus is proposed. The virus genome is composed of at least five RNA segments. Two coding regions were studied to determine isolate diversity with results pointing to a homogeneous virus population. Host range was evaluated using graft transmission and by testing species found in close proximity to infected trees. Mite transmission with Aculops cercidis, the predominant species found in redbud trees in the epicenter of the disease, was evaluated but was not found to be a vector of the virus. Based on this study and the accumulated knowledge on emaravirus evolution we propose that speciation is allopatric, with vectors being a major component of the process. (C) 2016 Elsevier B.V. All rights reserved. C1 [Di Bello, Patrick L.; Laney, Alma G.; Druciarek, Tobiasz; Ho, Thien; Gergerich, Rose C.; Tzanetakis, Ioannis E.] Univ Arkansas Syst, Div Agr, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Keller, Karen E.; Martin, Robert R.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Tzanetakis, IE (reprint author), Univ Arkansas Syst, Div Agr, Dept Plant Pathol, Fayetteville, AR 72701 USA. EM itzaneta@uark.edu OI Laney, Alma/0000-0001-6103-5731 FU Arkansas Agricultural Experimental Station; USDA Hatch project [1002361] FX The authors thank Dr. K.S. Kim for helpful discussions on the history of redbud yellow ringspot. This work was supported, at least in part, by the Arkansas Agricultural Experimental Station and USDA Hatch project 1002361. NR 52 TC 0 Z9 0 U1 6 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD AUG 15 PY 2016 VL 222 BP 41 EP 47 DI 10.1016/j.virusres.2016.05.027 PG 7 WC Virology SC Virology GA DT5QL UT WOS:000381537600006 PM 27262621 ER PT J AU Munyaneza, JE Mustafa, T Fisher, TW Sengoda, VG Horton, DR AF Munyaneza, Joseph E. Mustafa, Tariq Fisher, Tonja W. Sengoda, Venkatesan G. Horton, David R. TI Assessing the Likelihood of Transmission of Candidatus Liberibacter solanacearum to Carrot by Potato Psyllid, Bactericera cockerelli (Hemiptera: Triozidae) SO PLOS ONE LA English DT Article ID ZEBRA CHIP DISEASE; 1ST REPORT; UNITED-STATES; HOST-PLANTS; NEW-ZEALAND; PCR ASSAY; ASSOCIATION; TOMATO; INOCULATION; PSYLLOIDEA AB Candidatus Liberibacter solanacearum' (Lso) is a phloem-limited bacterium that severely affects important Solanaceae and Apiaceae crops, including potato, tomato, pepper, tobacco, carrot and celery. This bacterium is transmitted to solanaceous species by potato psyllid, Bactericera cockerelli, and to Apiaceae by carrot psyllids, including Trioza apicalis and Bactericera trigonica. Five haplotypes of Lso have so far been described, two are associated with solanaceous species and potato psyllids, whereas the other three are associated with carrot and celery crops and carrot psyllids. Little is known about cross-transmission of Lso to carrot by potato psyllids or to potato by carrot psyllids. Thus, the present study assessed whether potato psyllid can transmit Lso to carrot and whether Lso haplotypes infecting solanaceous species can also infect carrot and lead to disease symptom development. In addition, the stylet probing behavior of potato psyllid on carrot was assessed using electropenetrography (EPG) technology to further elucidate potential Lso transmission to Apiaceae by this potato insect pest. Results showed that, while potato psyllids survived on carrot for several weeks when confined on the plants under controlled laboratory and field conditions, the insects generally failed to infect carrot plants with Lso. Only three of the 200 carrot plants assayed became infected with Lso and developed characteristic disease symptoms. Lso infection in the symptomatic carrot plants was confirmed by polymerase chain reaction assay and Lso in the carrots was determined to be of the haplotype B, which is associated with solanaceous species. EPG results further revealed that potato psyllids readily feed on carrot xylem but rarely probe into the phloem tissue, explaining why little to no Lso infection occurred during the controlled laboratory and field cage transmission trials. Results of our laboratory and field transmission studies, combined with our EPG results, suggest that the risk of Lso infection and spread between psyllid-infested solanaceous and Apiaceae crops is likely to be negligible under normal field conditions. C1 [Munyaneza, Joseph E.; Mustafa, Tariq; Fisher, Tonja W.; Sengoda, Venkatesan G.; Horton, David R.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Munyaneza, JE (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Joseph.Munyaneza@ars.usda.gov FU United States Department of Agriculture (USDA)-Agricultural Research Service State Partnership Potato Research Program; Texas Department of Agriculture; USDA-National Institute of Food and Agriculture-Specialty Crop Research Initiative [2009-51181-20176]; USDA-ARS State Partnership Potato Research Program; USDA-NIFA-SCRI [2009-51181-20176] FX Financial support for this work was partially provided by United States Department of Agriculture (USDA)-Agricultural Research Service State Partnership Potato Research Program, Texas Department of Agriculture, and USDA-National Institute of Food and Agriculture-Specialty Crop Research Initiative (Project #2009-51181-20176). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank Kylie Swisher, Millie Heidt, Becky Cochran and Blaine Heilman for their invaluable technical support. Financial support for this work was partially provided by USDA-ARS State Partnership Potato Research Program, Texas Department of Agriculture, and USDA-NIFA-SCRI (Project #2009-51181-20176). The use of trade, firm, or corporation names in this publication is for information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer. NR 52 TC 0 Z9 0 U1 13 U2 14 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 15 PY 2016 VL 11 IS 8 AR e0161016 DI 10.1371/journal.pone.0161016 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT4SL UT WOS:000381471100030 PM 27525703 ER PT J AU Hwang, DH Kim, JA Lee, JY AF Hwang, Daniel H. Kim, Jeong-A. Lee, Joo Young TI Mechanisms for the activation of Toll-like receptor 2/4 by saturated fatty acids and inhibition by docosahexaenoic acid SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE Toll-like receptor; Inflammation; Saturated fatty acid; Polyunsaturated fatty acid; Docosahexaenoic acid ID MACROPHAGE CELL-LINE; LIPID RAFTS; INSULIN-RESISTANCE; DROSOPHILA EMBRYO; INFLAMMATORY RESPONSES; MEDIATED INFLAMMATION; ENDOTOXIN ANTAGONIST; SIGNALING PATHWAYS; TLR4-MD-2 COMPLEX; CRYSTAL-STRUCTURE AB Saturated fatty acids can activate Toll-like receptor 2 (TLR2) and TLR4 but polyunsaturated fatty acids, particularly docosahexaenoic acid (DHA) inhibit the activation. Lipopolysaccharides (LPS) and lipopetides, ligands for TLR4 and TLR2, respectively, are acylated by saturated fatty acids. Removal of these fatty acids results in loss of their ligand activity suggesting that the saturated fatty acyl moieties are required for the receptor activation. X-ray crystallographic studies revealed that these saturated fatty acyl groups of the ligands directly occupy hydrophobic lipid binding domains of the receptors (or co-receptor) and induce the dimerization which is prerequisite for the receptor activation. Saturated fatty acids also induce the dimerization and translocation of TLR4 and TLR2 into lipid rafts in plasma membrane and this process is inhibited by DHA. Whether saturated fatty acids induce the dimerization of the receptors by interacting with these lipid binding domains is not known. Many experimental results suggest that saturated fatty acids promote the formation of lipid rafts and recruitment of TLRs into lipid rafts leading to ligand independent dimerization of the receptors. Such a mode of ligand independent receptor activation defies the conventional concept of ligand induced receptor activation; however, this may enable diverse non-microbial molecules with endogenous and dietary origins to modulate TLR-mediated immune responses. Emerging experimental evidence reveals that TLRs play a key role in bridging diet-induced endocrine and metabolic changes to immune responses. Published by Elsevier B.V. C1 [Hwang, Daniel H.] Univ Calif Davis, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA. [Hwang, Daniel H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Kim, Jeong-A.] Univ Alabama Birmingham, Dept Med, Div Endocrinol Diabet & Metab, UAB Comprehens Diabet Ctr, Birmingham, AL 35294 USA. [Lee, Joo Young] Catholic Univ Korea, Coll Pharm, Bucheon 14662, South Korea. RP Hwang, DH (reprint author), USDA ARS, Dept Nutr, Fac Immunol Grad Grp, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA.; Hwang, DH (reprint author), Univ Calif Davis, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM daniel.hwang@ars.usda.gov FU USDA-ARS Program Project [5306-51530-017-00D]; USDA-ARS Program Project (USDA/NIFA competitive grant) [2013-03477]; NIH [HL128695]; American Diabetes Association [1-09-JF-33, 1-12-BS-99]; American Heart Association [13GRNT17220057]; National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning of Korea [NRF-2014R1A2A1A11051234]; UAB diabetes research center sponsored pilot and feasibility program - NIH [P30DK079626] FX This work was supported by USDA-ARS Program Project (5306-51530-017-00D and USDA/NIFA competitive grant 2013-03477 to D.H.H); NIH (HL128695 to J.K), the American Diabetes Association (1-09-JF-33; 1-12-BS-99 to J.K), American Heart Association (13GRNT17220057 to J. K), UAB diabetes research center sponsored pilot and feasibility program supported by NIH (P30DK079626); grant from the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning of Korea (NRF-2014R1A2A1A11051234 to J. Y. L.). NR 63 TC 4 Z9 4 U1 1 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 EI 1879-0712 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD AUG 15 PY 2016 VL 785 BP 24 EP 35 DI 10.1016/j.ejphar.2016.04.024 PG 12 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA DS4LP UT WOS:000380752900005 PM 27085899 ER PT J AU Wallsten, J Hatfield, R AF Wallsten, Johanna Hatfield, Ronald TI Cell wall chemical characteristics of whole-crop cereal silages harvested at three maturity stages SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE cereal crops; silages; oat; barley; wheat; cell walls; digestibility; lignin; structural carbohydrates ID BROMIDE SPECTROPHOTOMETRIC METHOD; ACID DETERGENT LIGNIN; ENZYMATIC DEGRADATION; KLASON LIGNIN; DAIRY HEIFERS; BARLEY SILAGE; MAIZE LIGNIN; DIGESTIBILITY; COUMAROYL; OATS AB BACKGROUND: In cooler climates such as found in Scandinavian countries cereals are important feedstuffs for ruminants often ensiled as whole-crop cereal silages (WCCS) to preserve nutrients. Animal performance varies with the type of cereal forage and stage of cereal development being ensiled. Cell wall isolation and analysis was undertaken to determine differences among cereal silages harvested at different stages of maturity. RESULTS: A set of 27 WCCS samples of barley, wheat and oats harvested at heading, early milk, and dough stages of maturity were analyzed for cell wall (CW) composition and compared to previous NDF analyses. Total CW concentrations of the WCCS were higher than the NDF concentration. The lignin concentration was higher (P < 0.001) in oats (111 g kg(-1) DM) than in barley (88 g kg(-1) DM) and wheat (91 g kg(-1) DM). Ferulates (ester and ether linked) ranged from 12.2 to 14.9 g kg(-1) across forage types and maturity stages. The correlation between total cell wall xylose and HC concentrations (NDF-ADF) was lower than expected in all forages (R = 0.63). CONCLUSION: The more comprehensive analyses of cell walls provide detailed composition of the different WCCS that vary due to the maturity and type of cereal. (C) 2016 The Authors. Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. C1 [Wallsten, Johanna] Swedish Univ Agr Sci, Dept Forest Biomat & Technol, SE-90183 Umea, Sweden. [Hatfield, Ronald] USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. RP Hatfield, R (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM Ronald.Hatfield@ars.usda.gov FU Swedish Research Council, FORMAS FX The authors wish to thank Jane Marita for assistance in overseeing the GC analysis of cell wall carbohydrate fractions from the silages. Thanks to the Swedish Research Council, FORMAS for providing financial support for JW. USDA is an equal opportunity provider and employer. Mention of a proprietary product does not constitute a recommendation or warranty of the product by USDA or Forest Biomaterials and Technology, Swedish University of Agricultural Sciences, Sweden, and does not imply approval to the exclusion of other suitable products. NR 48 TC 0 Z9 0 U1 16 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-5142 EI 1097-0010 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD AUG 15 PY 2016 VL 96 IS 10 BP 3604 EP 3612 DI 10.1002/jsfa.7736 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DP6VF UT WOS:000378636600040 PM 27029834 ER PT J AU Xu, B Pan, YD Plante, AF Johnson, A Cole, J Birdsey, R AF Xu, Bing Pan, Yude Plante, Alain F. Johnson, Arthur Cole, Jason Birdsey, Richard TI Decadal change of forest biomass carbon stocks and tree demography in the Delaware River Basin SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest biomass; Carbon stock; Tree demography; Delaware River Basin ID NORTHERN HARDWOOD FOREST; EASTERN UNITED-STATES; BEECH BARK DISEASE; LONG-TERM; ABOVEGROUND BIOMASS; CATSKILL MOUNTAINS; WORLDS FORESTS; BOREAL FOREST; US FORESTS; DYNAMICS AB Quantifying forest biomass carbon (C) stock change is important for understanding forest dynamics and their feedbacks with climate change. Forests in the northeastern U.S. have been a net carbon sink in recent decades, but C accumulation in some northern hardwood forests has been halted due to the impact of emerging stresses such as invasive pests, land use change and climate change. The Delaware River Basin (DRB), sited in the southern edge of the northern hardwood forest, features diverse forest types and land-use histories. In 2001-2003, the DRB Monitoring and Research Initiative established 61 forest plots in three research sites, using Forest Service inventory protocols and enhanced measurements. These plots were revisited and re-measured in 2012-2014. By comparing forest biomass C stocks in the two measurements, our results suggest that the biomass C stock of the DRB forest increased, and was thus a carbon sink over the past decade. The net biomass C stock change in the Neversink area in the north of the DRB was 1.94 Mg C ha(-1) yr(-1), smaller than the biomass C change in the French Creek area (2.52 Mg C ha(-1) yr(-1), southern DRB), and Delaware Water Gap Area (2.68 Mg C ha(-1) yr(-1), central ORB). An increase of dead biomass C accounted for 20% of the total biomass C change. The change of biomass C stocks did not correlate with any climatic or topographic factors, but decreased with increasing stand age, and with tree mortality rate. Mortality rates were highest in the smallest size class. In most of the major tree species, stem density decreased, but the loss of biomass from mortality was offset by recruitment and growth. The demographic changes differ dramatically among species. The living biomass of chestnut oak, white oak and black oak decreased because of the large mortality rate, while white pine, American beech and sweet birch increased in both biomass and stem density. Our results suggest that forests in the DRB could continue to be a carbon sink in the coming decades, because they are likely at a middle successional stage. The linkage between demography of individual trees species and biomass C change underscores the effects of species-specific disturbances such as non-native insects and pathogens on forest dynamics, and highlights the need for forest managers to anticipate these effects in their management plans. (C) 2016 Elsevier B.V. All rights reserved. C1 [Xu, Bing; Plante, Alain F.; Johnson, Arthur] Univ Penn, Dept Earth & Environm Sci, 40 South 33rd St, Philadelphia, PA 19104 USA. [Pan, Yude; Birdsey, Richard] US Forest Serv, USDA, No Res Stn, 11 Campus Blvd, Newtown Sq, PA 19073 USA. [Cole, Jason] US Forest Serv, USDA, No Res Stn, Syracuse, NY 13210 USA. RP Xu, B (reprint author), Univ Penn, Dept Earth & Environm Sci, 40 South 33rd St, Philadelphia, PA 19104 USA. EM xubing@sas.upenn.edu RI Plante, Alain/C-3498-2008 OI Plante, Alain/0000-0003-0124-6187 FU United States Forest Service [14-JV-11242306-083] FX This study was supported by United States Forest Service grant number 14-JV-11242306-083. We thank Lukas Jenkins, Adam Cesaneka, Jingyu Ji, Vanessa Eni, Ashley Crespo, Alexa Dugan, and Matthew Patterson for assistance in field sampling. We also acknowledge the private landowners that permitted access to their properties for field measurement. NR 66 TC 0 Z9 0 U1 13 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 1 EP 10 DI 10.1016/j.foreco.2016.04.045 PG 10 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700001 ER PT J AU Brose, PH AF Brose, Patrick H. TI Origin, development, and impact of mountain laurel thickets on the mixed-oak forests of the central Appalachian Mountains, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Dendrochronology; Forest regeneration; Kalmia latifolia; Quercus spp. ID NORTHERN RED-OAK; DECIDUOUS-ERICACEOUS SHRUBS; TREE SEEDLING RECRUITMENT; YELLOW-POPLAR SEEDLINGS; TALUS SLOPE FOREST; WHITE-TAILED DEER; RHODODENDRON-MAXIMUM; TEMPERATE FORESTS; SOUTHERN APPALACHIANS; EVERGREEN UNDERSTORY AB Throughout forests of the northern hemisphere, some species of ericaceous shrubs can form persistent understories that interfere with forest regeneration processes. In the Appalachian Mountains of eastern North America, mountain laurel (Kalmia latifolia) may interfere in the regeneration of mixed-oak (Quercus spp.) forests. To verify this possibility, I conducted a dendroecology study from 2001 to 2005 in three mixed-oak stands with mountain laurel thickets to elucidate how and when the thickets originated, developed, and were impacting hardwood seedlings. At all three sites, the oldest mountain laurel dated to the 1930s when the stands emerged from a period of recurring disturbance. However, most of the mountain laurel has originated since the 1950s when the stands were generally undisturbed. More recently, insect defoliations have accelerated the development of the thickets by increasing available sunlight. A strong negative relationship exists between the percent cover of mountain laurel and the density of hardwood seedlings with 20-30% cover being sufficient to inhibit seedling establishment and survival. Perpetuating mixed-oak forests that contain mountain laurel thickets will require reducing shrub cover to less than 20-30% at the beginning of the regeneration process to help ensure adequate densities of hardwood seedlings. Published by Elsevier B.V. C1 [Brose, Patrick H.] US Forest Serv, No Res Stn, 335 Natl Forge Rd, Irvine, PA 16329 USA. RP Brose, PH (reprint author), US Forest Serv, No Res Stn, 335 Natl Forge Rd, Irvine, PA 16329 USA. EM pbrose@fs.fed.us FU Joint Fire Science Program; Northern Research Station; Pennsylvania Bureau of Forestry FX I am indebted to the many people that contributed to this project. Among those, special thanks are due to Wendy Andersen, Brent Carlson, Ty Ryen, and Greg Sanford who did most of the field work in collecting the cores and cross sections and preparing them in the lab for analysis. I thank the Pennsylvania Bureau of Forestry, especially the staffs of the Clear Creek, Delaware, and Rothrock state forests, for permission to conduct the study on their property, access to the sites, and initial funding to start the project. Alex Royo, Tom Schuler, Susan Stout, Tom Waldrop, and three anonymous reviewers provided comments of early drafts that helped with clarity and conciseness. John Stanovick assisted with statistical analysis. The Joint Fire Science Program and the Northern Research Station provided subsequent funding to allow completion of the study. NR 72 TC 0 Z9 0 U1 19 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 33 EP 41 DI 10.1016/j.foreco.2016.04.040 PG 9 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700004 ER PT J AU Reilly, MJ Spies, TA AF Reilly, Matthew J. Spies, Thomas A. TI Disturbance, tree mortality, and implications for contemporary regional forest change in the Pacific Northwest SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Tree mortality; Pacific Northwest; Disturbance; Regional forest dynamics; Fire; Insects ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; FALSE DISCOVERY RATE; MIXED-SEVERITY FIRE; SUB-ALPINE FOREST; CLIMATE-CHANGE; CONIFEROUS FORESTS; PHYSICAL DAMAGE; USA; DROUGHT AB Tree mortality is an important demographic process and primary driver of forest dynamics, yet there are relatively few plot-based studies that explicitly quantify mortality and compare the relative contribution of endogenous and exogenous disturbances at regional scales. We used repeated observations on 289,390 trees in 3673 1 ha plots on U.S. Forest Service lands in Oregon and Washington to compare distributions of mortality rates among natural disturbances and vegetation zones from the mid-1990s to mid-2000s, a period characterized by drought, insect outbreaks, and large wildfires. Endogenous disturbances (e.g. pathogens, insects) were pervasive but operated at relatively low levels of mortality (<2.5%/yr) that rarely exceeded 5%/yr. Exogenous disturbances (e.g. fire, wind, landslides, avalanches) were less common and operated mostly at intermediate levels of mortality (5-25%/yr) indicative of partial-stand replacement events. Stand-replacing mortality rates (>= 25%/yr) comprised a third of all exogenous disturbance events, occurring almost exclusively in fires. Fires were rare in wet vegetation zones and most rates were <2.5%/yr and associated with endogenous processes. Mortality rates in dry vegetation zones revealed a different set of dynamics including a more variable role of background mortality and greater proportions of mortality associated with fire and insects at partial- and stand-replacing levels. Mortality rates in early and middle stages of stand development were low compared to published rates, but rates >1%/yr in over half of the plots in late and old-growth stages corroborate previous findings of elevated mortality during the same period and indicate the potential for pervasive structural change across all vegetation zones. Partial- and stand-replacing fire were associated with most mortality, but affected a relatively small proportion of dry vegetation zones (3.1-7.1% and 2.1-5.1%, respectively). These disturbances have likely affected regional biodiversity through the creation of early seral habitat, increased within-stand heterogeneity, and restored some aspects of historical fire regimes, but there is a need to better understand corresponding structural and compositional changes. We demonstrate the variability in the drivers, magnitude, and extent of mortality across a biophysically diverse region and highlight the need to incorporate and characterize the effects of mortality at intermediate levels to develop a more comprehensive understanding of regional forest dynamics. (c) 2016 Elsevier B.V. All rights reserved. C1 [Reilly, Matthew J.] Oregon State Univ, Coll Forestry, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Spies, Thomas A.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Reilly, MJ (reprint author), Oregon State Univ, Coll Forestry, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM matthew.reilly@oregonstate.edu FU USDA Forest Service Region 6 Inventory and Analysis program FX Funding for this work was provided by the USDA Forest Service Region 6 Inventory and Analysis program. We thank Andy Gray and Matt Gregory for their assistance with inventory data and John Bailey, Matt Betts, Patricia Muir, David L. Peterson, Dave Shaw, Dave Bell and two anonymous reviewers for comments that greatly improved the final version of manuscript. NR 74 TC 1 Z9 1 U1 23 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 102 EP 110 DI 10.1016/j.foreco.2016.05.002 PG 9 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700011 ER PT J AU Phillips, J Duque, A Scott, C Wayson, C Galindo, G Cabrera, E Chave, J Pena, M Alvarez, E Cardenas, D Duivenvoorden, J Hildebrand, P Stevenson, P Ramirez, S Yepes, A AF Phillips, Juan Duque, Alvaro Scott, Charles Wayson, Craig Galindo, Gustavo Cabrera, Edersson Chave, Jerome Pena, Miguel Alvarez, Esteban Cardenas, Dairon Duivenvoorden, Joost Hildebrand, Patricio Stevenson, Pablo Ramirez, Sebastian Yepes, Adriana TI Live aboveground carbon stocks in natural forests of Colombia SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Carbon stocks; Aboveground biomass; Natural forests; Colombia; Allometric equations; Estimation method ID TROPICAL FORESTS; BIOMASS ESTIMATION; AMAZONIAN FORESTS; EMISSIONS; DENSITY; FIELD; DEFORESTATION; PRODUCTIVITY; ALLOMETRY; TREES AB Emission factors are essential in order to accurately account and report land use and land use change emissions due to deforestation at a national level, in accordance with the United Nations Framework Convention on Climate Change reporting guidelines. Nonetheless, in many tropical countries its availability is still scarce, especially in Colombia where a National Forest Inventory is lacking. Here, we estimate the amount of carbon stored in the live aboveground biomass of the forests of Colombia, using data from 4981 sampling forest plots of various sizes established between 1990 and 2014. Our study included an analysis of the influence of the choice of allometric model and the carbon density estimation method employed in the estimation. We found that the most conservative total mean value for the entire country was 226.9 +/- 4.5 Mg ha(-1), obtained by using a previous set of allometric equations developed for the natural forest of Colombia and an inverse-variance weighting that accounts for the variation in plot size, which represents a potential stock of 6.44 +/- 0.13 Pg of carbon. Thus, our study provides a method to utilize existing sample data to assess forest carbon stocks at national level, make available conservative carbon stocks estimates for the natural forests of Colombia, and reports enhanced and adequate information subject to national capabilities and policies in the context of results-based payments for reducing emissions from deforestation and forest degradation and the conservation. (c) 2016 Elsevier B.V. All rights reserved. C1 [Phillips, Juan; Galindo, Gustavo; Cabrera, Edersson; Pena, Miguel; Yepes, Adriana] Inst Hidrol Meteorol & Estudios Ambientales IDEAM, Calle 25D 96B-70, Bogota, Colombia. [Phillips, Juan; Pena, Miguel] Univ Nacl Colombia, Ecol, Calle 59A 63-20, Medellin, Colombia. [Phillips, Juan] MADS, Calle 37 8-40, Bogota, Colombia. [Duque, Alvaro; Ramirez, Sebastian] Univ Nacl Colombia, Dept Ciencias Forestales, Calle 59A 63-20, Medellin, Colombia. [Scott, Charles] US Forest Serv, Forest Inventory & Anal, No Res Stn, USDA, 11 Campus Blvd,Suite 200, Newtown Sq, PA USA. [Wayson, Craig] USDA Forest Serv, Latin Amer Reg SilvaCarbon Programme, Calle Antequera 777, Lima, Peru. [Chave, Jerome] CNRS UPS, Lab Evolut & Diversite Biol, Baatiment 4R3,118 Route Narbonne, F-31062 Toulouse, France. [Alvarez, Esteban] Jardin Bot Medellin Joaquin Antonio Uribe, Calle 73 51D-14, Medellin, Colombia. [Alvarez, Esteban] Univ Alcala de Henares, Cambio Global & Desarrollo Sostenible, Plaza San Diego, Madrid 28801, Spain. [Cardenas, Dairon] Inst Amazon Invest Cient SINCHI, Calle 20 5-44, Bogota, Colombia. [Duivenvoorden, Joost] Univ Amsterdam, IBED, Kruislaan 318, NL-1098 SM Amsterdam, Netherlands. [Hildebrand, Patricio] Fdn Puerto Rastrojo, Carrera 10 24-76, Bogota, Colombia. [Stevenson, Pablo] Univ Los Andes, Dept Ciencias Biol, Carrera 1 18A-12, Bogota, Colombia. [Yepes, Adriana] Ctr Invest Ecosistemas & Cambio Global Carbono &, Calle 51A 72-23, Medellin, Colombia. RP Phillips, J (reprint author), MADS, Calle 37 8-40, Bogota, Colombia. EM j.f.phillips.bernal@gmail.com FU project "Consolidacion de un Sistema de Monitoreo de Bosques y Carbono, como soporte a la Politica Ambiental y de Manejo en Colombia"; Gordon and Betty Moore Foundation; "Investissement d'Avenir" grants [CEBA: ANR-10-LABX-25-01, TULIP: ANR-10-LABX-0041] FX We thank Maria Claudia Garcia and Maria Saralux Valbuena for their extensive help and support, and Kenneth Cabrera for his statistical advice on previous versions of this manuscript. We are indebted to all researchers, institutions and local people that participated in the plot survey and contributed with data for this publication. We thank our IDEAM teammates for the time, energy, and great talents that they put into the developing and implementation of the digital image processing protocol. We thank the anonymous reviewers for their insightful comments and suggestions, which helped us to improve the manuscript. Juan Phillips, Alvaro Duque, Gustavo Galindo, Edersson Cabrera, Miguel Pena, and Adriana Yepes, received funding from the project "Consolidacion de un Sistema de Monitoreo de Bosques y Carbono, como soporte a la Politica Ambiental y de Manejo en Colombia", which was implemented by IDEAM with the financial support of the Gordon and Betty Moore Foundation. Jerome Chave was supported by "Investissement d'Avenir" grants managed by Agence Nationale de la Recherche (CEBA: ANR-10-LABX-25-01; TULIP: ANR-10-LABX-0041). NR 71 TC 2 Z9 2 U1 16 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 119 EP 128 DI 10.1016/j.foreco.2016.05.009 PG 10 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700013 ER PT J AU Flathers, KN Kolb, TE Bradford, JB Waring, KM Moser, WK AF Flathers, Kelsey N. Kolb, Thomas E. Bradford, John B. Waring, Kristen M. Moser, W. Keith TI Long-term thinning alters ponderosa pine reproduction in northern Arizona SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Pinus ponderosa; Regeneration; Seedling establishment; Silviculture; Soil water content ID WESTERN UNITED-STATES; TREE MORTALITY; RESTORATION TREATMENTS; FOREST REGENERATION; BURNING TREATMENTS; CLIMATE-CHANGE; DROUGHT; ESTABLISHMENT; RECRUITMENT; GROWTH AB The future of ponderosa pine (Pinus ponderosa var. scopulorum) forests in the southwestern United States is uncertain because climate-change-induced stresses are expected to increase tree mortality and place greater constraints on regeneration. Silvicultural treatments, which include thinning, are increasingly being used to address forest health concerns by restoring ponderosa pine forests to more open conditions representative of historical forest structure. In light of the greater use of thinning and mounting concerns about the future of the species at the southern edge of its range, further investigations about impacts of thinning on ponderosa pine regeneration and underlying mechanisms are needed. We used a long-term (>50 years) experiment in northern Arizona to investigate impacts of repeated stand thinning that maintained different growing stock basal areas (0, 7, 14, 23, 34, 66 m(2) ha(-1)) on early seedling survival, growth, and microenvironment. Seedling survival for the first two years after germination (2013-2015), which had above-average precipitation, was higher than reported in several earlier studies and ranged between 4 and 21% among all basal areas. Seedling density exhibited a negative quadratic relationship with basal area and was positively associated with litter cover. Growing stock levels that fostered the highest seedling survival and density were those with a low density of overstory trees, low canopy cover, high cone production, coverage of soil by a thin layer of litter, and high soil water content at a depth of 15-30 cm. Overstory basal area was positively associated with seedling height but negatively associated with seedling diameter. During this relatively wet period, all basal area treatments supported higher average seedling densities than those previously recommended to produce a multi-aged stand or presettlement structure in the southwestern United States. Our results show that long-term maintenance of low to intermediate basal areas (7-23 m(2) ha(-1)) by thinning over the last 50 years led to a favorable microenvironment for early seedling establishment of ponderosa pine. (c) 2016 Elsevier B.V. All rights reserved. C1 [Flathers, Kelsey N.; Kolb, Thomas E.; Waring, Kristen M.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Bradford, John B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Moser, W. Keith] US Forest Serv, USDA, Rocky Mt Res Stn, Forest & Woodland Ecosyst Sci, Flagstaff, AZ USA. RP Kolb, TE (reprint author), No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. EM tom.kolb@nau.edu RI Bradford, John/E-5545-2011 FU United States Geological Survey [G13AC00268]; USGS Ecosystems mission area; Climate and Landuse Change mission area FX The project described in this publication was supported by Grant/Cooperative Agreement Number G13AC00268 from the United States Geological Survey to Northern Arizona University. JBB was supported by the USGS Ecosystems mission area and the Climate and Landuse Change mission area. We thank Ashley Wilde man and Megan Sullivan for help with data collection, and two anonymous reviewers for their helpful suggestions. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 78 TC 2 Z9 2 U1 20 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 154 EP 165 DI 10.1016/j.foreco.2016.04.053 PG 12 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700017 ER PT J AU Bohlman, GN North, M Safford, HD AF Bohlman, Gabrielle N. North, Malcolm Safford, Hugh D. TI Shrub removal in reforested post-fire areas increases native plant species richness SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Mixed-conifer forest; High-severity fire; Reforestation; Richness; Understory composition ID NORTHERN SIERRA-NEVADA; MIXED-CONIFER FOREST; UNDERSTORY VEGETATION; PINUS-PONDEROSA; CASCADE MOUNTAINS; BURN SEVERITY; FIRE; CALIFORNIA; SUCCESSION; USA AB Large, high severity fires are becoming more prevalent in Sierra Nevada mixed-conifer forests, largely due to heavy fuel loading and forest densification caused by past and current management practices. In post fire areas distant from seed trees, conifers are often planted to re-establish a forest and to prevent a potential type-conversion to shrub fields. Typical reforestation efforts promote conifer survival and growth by reducing competing shrub cover, yet the effects of these practices on plant species richness and composition are not well understood. We compared the effects of treatment and time since fire on (1) native and exotic plant species richness, (2) plant community composition, and (3) stand structure. Plots were installed throughout three different aged but proximate fires located in the canyon of the South Fork of the American River in California, 10, 22, and 41 years after fire. All three fires included large patches of stand-replacing fire that had been reforested with conifers as well as unplanted areas. Native plant species richness was significantly higher in planted areas where shrub cover was lower and planted trees successfully established. Native species richness decreased as time since fire increased, but the relationship between shrub control and richness persisted. Exotic species richness was higher on treated sites in the more recent fires, while the opposite was true in the oldest fire. As time since fire increased, under story species composition shifted from a community dominated by annuals and perennials to one dominated by shrubs and shade-tolerant trees. Shrub cover and July soil moisture were the top two factors influencing understory richness levels. Natural regeneration was low in the youngest fire and high in the oldest fire but highly heterogeneous in all three fires. Our study suggests that while retaining some shrub cover for post-fire habitat may be desirable, some level of shrub reduction does favor native plant richness and overall herbaceous cover. (c) 2016 Elsevier B.V. All rights reserved. C1 [Bohlman, Gabrielle N.; North, Malcolm] Univ Calif Davis, Dept Plant Sci, 1 Shields Ave, Davis, CA 95616 USA. [North, Malcolm] US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. [Safford, Hugh D.] US Forest Serv, USDA, Pacific Southwest Reg, 1323 Club Dr, Vallejo, CA 94592 USA. [Safford, Hugh D.] Univ Calif Davis, Dept Environm Sci & Policy, 1 Shields Ave, Davis, CA 95616 USA. RP Bohlman, GN (reprint author), Univ Calif Davis, Dept Plant Sci, 1 Shields Ave, Davis, CA 95616 USA. EM gnbohlman@ucdavis.edu FU USDA Forest Service; Eldorado National Forest; Pacific Southwest Region FX We thank Haley Wiggins, Debra Scolnick, Ian Medeiros, Paul Excoffier, Kaya MacMillen, Maisie Borg and Jason Barton for assisting in data collection. Thanks to Don Errington, Bob Carroll, Duane Nelson, and Jeff Griffin for sharing their knowledge on the reforestation activities throughout the Freds and Cleveland fire areas and Brian Levine for providing select copies of the Pilliken documentation that was lost in the warehouse fire. Butch Geyer also provided invaluable insight on management of the Pilliken Fire area. We greatly appreciate Becky Estes' help in securing funding for this project and Mason Earles for assisting with the data analysis. Thank you to our reviewers for their help in greatly improving this manuscript. Funding was provided by the USDA Forest Service, Eldorado National Forest and Pacific Southwest Region. NR 88 TC 3 Z9 3 U1 14 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 195 EP 210 DI 10.1016/j.foreco.2016.05.008 PG 16 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700021 ER PT J AU Lee, CA Dey, DC Muzika, RM AF Lee, Christopher A. Dey, Daniel C. Muzika, Rose-Marie TI Oak stump-sprout vigor and Armillaria infection after clearcutting in southeastern Missouri, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Root disease; Tree vigor; Sprout competition; Infection; Compartmentalization; Regeneration; Disease management ID COMPETITIVE SUCCESS; MORTALITY; FORESTS; REGENERATION; DECLINE; ROOT; OVERSTORY; PATTERNS; DISEASE; MELLEA AB Armillaria spp. occur widely in Missouri mixed-oak ecosystems. In order to better understand the ecology and management of this pathogen and its effects on oak coppice, we observed a transect of 150 stumps after clearcutting in southeastern Missouri, noting Armillaria infection and oak sprout demography one year and seven years after harvest. Additionally, we visited a 50-year-old clearcutting in the same area to sample oak root systems of stump-sprout origin for comparison with the seven-year-old clearcut. One year after harvest, 55% of stumps supported Armillaria infections, while 62% of stumps were infected after seven years. In the 50-year-old clearcut, 21% of examined root systems were infected. Logistic regression analysis of the younger clearcut related likelihood of infection to tree age at time of harvest. Whereas Armillaria infection displayed weak to absent relationships with numbers of sprouts surviving over time, dominant sprout height and diameter on individual stumps proxies for stump vigor were positively associated with numbers of survivors. These measures of vigor also had more influence over the magnitude and development of sproutless gaps around the circumference of the stump than did Armillaria infection. Moreover, the random effect of the individual tree on the development of such gaps was large. These results point to an important role for individual stump vigor in regulating sprout self-thinning rates, potentially through compartmentalization of invading Armillaria. (c) 2016 Elsevier B.V. All rights reserved. C1 [Lee, Christopher A.; Muzika, Rose-Marie] Univ Missouri, Sch Nat Resources, Dept Forestry, 203 ABNR Bldg, Columbia, MO 65211 USA. [Dey, Daniel C.] US Forest Serv, USDA, No Res Stn, 202 ABNR Bldg, Columbia, MO 65211 USA. RP Lee, CA (reprint author), Univ Missouri, Dept Forestry, 203 ABNR Bldg, Columbia, MO 65119 USA. EM calyt3@mail.missouri.edu; ddey@fs.fed.us; muzika@missouri.edu FU USDA Forest Service Northern Research Station; University of Missouri Life Science FX We are grateful to the following individuals for help with access, materials, and methods: John Kabrick and Texas Nall (USDA Forest Service); Jeanne Mihail, Susan Taylor, Jim English, Ricardo Holdo, and Sharon Reed (University of Missouri); the USDA Forest Service Northern Research Station; and the University of Missouri Life Science Fellowship and Research Council Grant programs. We thank Johann Bruhn and Kathy Kromroy for originating this study and performing the first phase including species identifications. NR 61 TC 0 Z9 0 U1 9 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2016 VL 374 BP 211 EP 219 DI 10.1016/j.foreco.2016.05.014 PG 9 WC Forestry SC Forestry GA DP3AB UT WOS:000378363700022 ER PT J AU Elkin, KR Slingsby, R Bryant, RB AF Elkin, Kyle R. Slingsby, Rosanne Bryant, Ray B. TI Determination of phytate in high molecular weight, charged organic matrices by two-dimensional size exclusion-ion chromatography SO TALANTA LA English DT Article DE Size exclusion chromatography; Ion chromatography; Two-dimensional chromatography; Matrix removal; Phytate ID LIQUID-CHROMATOGRAPHY; PHYTIC ACID; PHOSPHORUS; EXCHANGE; EXTRACTS; SPECIATION; PRODUCTS; REMOVAL; MATTER AB A two-dimensional chromatography method for analyzing phytate or other ionic targets in matrices containing high molecular weight, charged organic species is described. Prior to quantification by anion exchange chromatography, the sample matrix was prepared by size exclusion chromatography, which removed the majority of the matrix. Quantification of phytate on the AS11-HC was sensitive (0.25 mu M, 0.17 mg/l) and reproducible (4.6% RSD) allowing this method to provide baseline separation of phytate from a manure extract within 14 min. The method is simple, requiring only sample filtering, reproducible (between-run variation 5% RSD) and linear from 0.38 to 76 mu M (0.25-50 mg/l). The method is suitable for routine determination of phytate in high organic matrices such as manure extracts. Published by Elsevier B.V. C1 [Elkin, Kyle R.; Bryant, Ray B.] USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702,Curtin Rd, University Pk, PA 16802 USA. [Slingsby, Rosanne] Thermo Fisher Sci, 1228 Titan Way, Sunnyvale, CA 94088 USA. RP Elkin, KR (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702,Curtin Rd, University Pk, PA 16802 USA. EM kyle.elkin@ars.usda.gov FU Thermo-Fisher Dionex FX Many thanks to Thermo-Fisher Dionex for their support and cooperation throughout this project. The U.S. Department of Agriculture is an equal opportunity provider and employer. Mention of trade names does not constitute endorsement by USDA. NR 22 TC 0 Z9 0 U1 9 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0039-9140 EI 1873-3573 J9 TALANTA JI Talanta PD AUG 15 PY 2016 VL 156 BP 6 EP 10 DI 10.1016/j.talanta.2016.04.059 PG 5 WC Chemistry, Analytical SC Chemistry GA DP0MC UT WOS:000378182900002 PM 27260428 ER PT J AU Yi, J Fan, YT Zhang, YZ Zhao, LQ AF Yi, Jiang Fan, Yuting Zhang, Yuzhu Zhao, Liqing TI Characterization of catechin-alpha-lactalbumin conjugates and the improvement in beta-carotene retention in an oil-in-water nanoemulsion SO FOOD CHEMISTRY LA English DT Article DE alpha-Lactalbumin; Nano-encapsulation; beta-Carotene; SDS-PAGE; Chemical stability ID FUNCTIONAL-PROPERTIES; ANTIOXIDANT ACTIVITY; CHEMICAL-STABILITY; LIPID-PEROXIDATION; CELLULAR UPTAKE; SERUM-ALBUMIN; TEA CATECHINS; WHEY-PROTEIN; LACTOGLOBULIN; ACID AB The goal of this study was to prepare and characterize alpha-lactalbumin (ALA)-catechin conjugates as a novel emulsifier in improving the retention of beta-carotene (BC) in nanoemulsions via a free radical method. Covalent modification was observed and at least one catechin molecule was binding with ALA according to ESI-MS results. Far-UV CD indicated that the secondary structure of ALA was changed after conjugation. The Z-average particle diameters of nanoemulsions stabilized with ALA and ALA-catechin conjugates were 158.8 and 162.7 nm, respectively. The increase of mean particle size and the degradation of BC at 50 degrees C were both larger than at 25 degrees C during 30 days storage. BC retention stabilized with ALA-catechin conjugates was appreciably greater than ALA (control), which was attributed to the increase of ALA's radicals-scavenging and free metal ion binding ability after grafting with catechin. The chemical antioxidant activities of ALA-catechin conjugates were increased with increasing concentrations from 0.1 to 1.0 mg/ml. In general, labile phytochemicals, like BC, can be protected against oxidation during storage by proteins-polyphenols conjugates without any side effects. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yi, Jiang; Zhao, Liqing] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. [Fan, Yuting] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China. [Zhang, Yuzhu] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Yi, J; Zhao, LQ (reprint author), Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Peoples R China. EM yijiangjnu@gmail.com; snowyzlj@163.com FU Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program [JCYJ20140418091413576, CXZZ20150430093131635] FX This work was supported by the Shenzhen Dedicated Funding of Strategic Emerging Industry Development Program (JCYJ20140418091413576, CXZZ20150430093131635). NR 44 TC 2 Z9 2 U1 24 U2 104 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD AUG 15 PY 2016 VL 205 BP 73 EP 80 DI 10.1016/j.foodchem.2016.03.005 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA DG8SX UT WOS:000372355000011 PM 27006216 ER PT J AU Wu, J Li, X Fang, H Yi, YQ Chen, D Long, Y Gao, XX Wei, XY Chen, CYO AF Wu, Jie Li, Xue Fang, Hua Yi, Yanqun Chen, Dan Long, Yan Gao, Xinxin Wei, Xiaoyong Chen, C-Y. Oliver TI Investigation of synergistic mechanism and identification of interaction site of aldose reductase with the combination of gigantol and syringic acid for prevention of diabetic cataract SO BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE LA English DT Article DE Synergism; Diabetic cataract; Site-directed mutagenesis; Gigantol; Syringic acid; Aldose reductase ID LENS EPITHELIAL-CELLS; AURANTIACUM-VAR.-DENNEANUM; GLYCATION END-PRODUCTS; KINASE-C ACTIVATION; NITRIC-OXIDE; OXIDATIVE STRESS; GALACTOSEMIC RATS; SORBITOL PATHWAY; OSMOTIC-STRESS; IN-VITRO AB Background: Gigantol and syringic acid (SA) have been shown to synergistically prevent formation of diabetic cataract (DC). However, the exact mechanism of this effect is unknown. Here, we investigate the effect of these compounds on the activity of aldose reductase (AR) and cataract formation. Methods: We examined the synergistic anti-cataract efficacy of gigantol and SA in the high glucose-and streptozotocin - induced DC rat model; synergism was evaluated using Jin's formula. We investigated possible mechanisms of action by measuring AR expression and activity and levels of sorbitol using enzyme kinetics, Western blot, and RT-PCR. Finally, we examined binding interaction between AR and both compounds using a combination of site-directed mutagenesis, recombinant expression of wild-type and mutant proteins, and enzyme kinetics. Results: Combination treatment of gigantol and SA synergistically protected both HLECs(human lens epithelial cells) grown in vitro and DC formation in STZ-induced rats in vivo. Synergism was attributed to inhibition of AR activity, downregulation of AR expression via impaired transcription, and decreased sorbitol levels. Enzyme kinetics studies showed that the activity of an AR Asn160Ala mutant protein was significantly decreased compared to wild-type AR, confirming that Asn160 is a key residue for interaction between AR and both compounds. Conclusion: Combined administration of gigantol and SA synergize to enhance anti-cataract efficacy. The synergistic effect is mainly attributed to disruption of the polyol pathway and inhibition of AR activity. C1 [Wu, Jie; Li, Xue; Fang, Hua; Yi, Yanqun; Chen, Dan; Long, Yan; Gao, Xinxin; Wei, Xiaoyong] Guangzhou Univ Chinese Med, Dept Histol & Embryol, Guangzhou 510006, Guangdong, Peoples R China. [Wei, Xiaoyong; Chen, C-Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. RP Fang, H; Wei, XY (reprint author), Guangzhou Univ Chinese Med, Dept Histol & Embryol, Guangzhou 510006, Guangdong, Peoples R China.; Wei, XY (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. EM hlfh@gzucm.edu.cn; yaojidewxy@sina.com FU National Natural Science Foundation of China [81274157, 81102674, 30850012]; Guangdong Natural Science Foundation [S2011010005661]; Guangdong Science & Technology Planning Project [2011B031700076, 2009B090300335]; Guangzhou Science & Technology Planning Project [2014 J4100082]; U.S. Department of Agriculture (USDA)/Agricultural Research Service [1950-5100-087] FX This study was supported by grants from the National Natural Science Foundation of China (81274157, 81102674, 30850012), Guangdong Natural Science Foundation (S2011010005661), Guangdong Science & Technology Planning Project (2011B031700076, 2009B090300335), Guangzhou Science & Technology Planning Project (2014 J4100082) and the U.S. Department of Agriculture (USDA)/Agricultural Research Service (Cooperative Agreement No. 1950-5100-087). NR 90 TC 0 Z9 0 U1 9 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1472-6882 J9 BMC COMPLEM ALTERN M JI BMC Complement. Altern. Med. PD AUG 12 PY 2016 VL 16 AR 286 DI 10.1186/s12906-016-1251-5 PG 14 WC Integrative & Complementary Medicine SC Integrative & Complementary Medicine GA DT6AS UT WOS:000381565500004 PM 27520089 ER PT J AU Martin, K Singh, J Hill, JH Whitham, SA Cannon, SB AF Martin, Kathleen Singh, Jugpreet Hill, John H. Whitham, Steven A. Cannon, Steven B. TI Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.) SO BMC GENOMICS LA English DT Article DE Gene expression; Gene regulation; Host-virus interaction; Regulatory changes; Phaseolus vulgaris; Bean common mosaic virus ID GENOME-WIDE ANALYSIS; ALTERNATIVE SPLICING LANDSCAPE; RNA-SEQ; DISEASE RESISTANCE; DEFENSE RESPONSE; PATHOGEN INTERACTIONS; MEDICAGO-TRUNCATULA; FAMILY POTYVIRIDAE; DIFFERENTIAL GENE; STRESS RESPONSES AB Background: Bean common mosaic virus (BCMV) is widespread, with Phaseolus species as the primary host plants. Numerous BCMV strains have been identified on the basis of a panel of bean varieties that distinguish the pathogenicity types with respect to the viral strains. The molecular responses in Phaseolus to BCMV infection have not yet been well characterized. Results: We report the transcriptional responses of a widely susceptible variety of common bean (Phaseolus vulgaris L., cultivar 'Stringless green refugee') to two BCMV strains, in a time-course experiment. We also report the genome sequence of a previously unreported BCMV strain. The interaction with the known strain NL1-Lowa causes moderate symptoms and large transcriptional responses, and the newly identified strain (Strain 2 or S2) causes severe symptoms and moderate transcriptional responses. The transcriptional profiles of host plants infected with the two isolates are distinct, and involve numerous differences in splice forms in particular genes, and pathway specific expression patterns. Conclusions: We identified differential host transcriptome response after infection of two different strains of Bean common mosaic virus (BCMV) in common bean (Phaseolus vulgaris L.). Virus infection initiated a suite of changes in gene expression level and patterns in the host plants. Pathways related to defense, gene regulation, metabolic processes, photosynthesis were specifically altered after virus infection. Results presented in this study can increase the understanding of host-pathogen interactions and provide resources for further investigations of the biological mechanisms in BCMV infection and defense. C1 [Martin, Kathleen] Kansas State Univ, Dept Plant Pathol, Throckmorton Hall, Manhattan, KS 66506 USA. [Singh, Jugpreet] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Hill, John H.; Whitham, Steven A.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Cannon, Steven B.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Cannon, Steven B.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Crop Genome Informat Lab, Ames, IA 50011 USA. RP Cannon, SB (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA.; Cannon, SB (reprint author), Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Crop Genome Informat Lab, Ames, IA 50011 USA. EM steven.cannon@ars.usda.gov FU USDA-ARS [5030-21000-062-00D] FX Funding for this study was from the USDA-ARS CRIS project, SoyBase and the Legume Clade Database, project 5030-21000-062-00D. Design of the study and collection, analysis, and interpretation of data and in writing the manuscript was at the discretion of the authors. NR 83 TC 0 Z9 0 U1 8 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 11 PY 2016 VL 17 AR 613 DI 10.1186/s12864-016-2976-8 PG 19 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DX0JP UT WOS:000384047900005 PM 27515794 ER PT J AU Schneider, R Rolling, W Song, QJ Cregan, P Dorrance, AE McHale, LK AF Schneider, Rhiannon Rolling, William Song, Qijian Cregan, Perry Dorrance, Anne E. McHale, Leah K. TI Genome-wide association mapping of partial resistance to Phytophthora sojae in soybean plant introductions from the Republic of Korea SO BMC GENOMICS LA English DT Article DE Glycine max; GWAS; Haplotype; Linkage disequilibrium; Partial resistance; Phytophthora sojae; Single nucleotide polymorphism ID QUANTITATIVE TRAIT LOCI; SUDDEN-DEATH SYNDROME; CONFERRING PARTIAL RESISTANCE; NEMATODE HETERODERA-GLYCINES; CYST-NEMATODE; DISEASE RESISTANCE; ROOT-ROT; SCLEROTINIA-SCLEROTIORUM; UNDERLYING RESISTANCE; EXPRESSION ANALYSIS AB Background: Phytophthora root and stem rot is one of the most yield-limiting diseases of soybean [Glycine max (L.) Merr], caused by the oomycete Phytophthora sojae. Partial resistance is controlled by several genes and, compared to single gene (Rps gene) resistance to P. sojae, places less selection pressure on P. sojae populations. Thus, partial resistance provides a more durable resistance against the pathogen. In previous work, plant introductions (PIs) originating from the Republic of Korea (S. Korea) have shown to be excellent sources for high levels of partial resistance against P. sojae. Results: Resistance to two highly virulent P. sojae isolates was assessed in 1395 PIs from S. Korea via a greenhouse layer test. Lines exhibiting possible Rps gene immunity or rot due to other pathogens were removed and the remaining 800 lines were used to identify regions of quantitative resistance using genome-wide association mapping. Sixteen SNP markers on chromosomes 3, 13 and 19 were significantly associated with partial resistance to P. sojae and were grouped into seven quantitative trait loci (QTL) by linkage disequilibrium blocks. Two QTL on chromosome 3 and three QTL on chromosome 19 represent possible novel loci for partial resistance to P. sojae. While candidate genes at QTL varied in their predicted functions, the coincidence of QTLs 3-2 and 13-1 on chromosomes 3 and 13, respectively, with Rps genes and resistance gene analogs provided support for the hypothesized mechanism of partial resistance involving weak R-genes. Conclusions: QTL contributing to partial resistance towards P. sojae in soybean germplasm originating from S. Korea were identified. The QTL identified in this study coincide with previously reported QTL, Rps genes, as well as novel loci for partial resistance. Molecular markers associated with these QTL can be used in the marker-assisted introgression of these alleles into elite cultivars. Annotations of genes within QTL allow hypotheses on the possible mechanisms of partial resistance to P. sojae. C1 [Schneider, Rhiannon; McHale, Leah K.] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA. [Schneider, Rhiannon] Pioneer HiBred Int Inc, Napoleon, OH 43545 USA. [Rolling, William; Dorrance, Anne E.; McHale, Leah K.] Ohio State Univ, Ctr Appl Plant Sci, Columbus, OH 43210 USA. [Song, Qijian; Cregan, Perry] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Dorrance, Anne E.] Ohio State Univ, Dept Plant Pathol, Wooster, OH 44691 USA. RP McHale, LK (reprint author), Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA.; McHale, LK (reprint author), Ohio State Univ, Ctr Appl Plant Sci, Columbus, OH 43210 USA. EM mchale.21@osu.edu FU United Soybean Board; Ohio Soybean Council; Ohio State University Center for Applied Plant Sciences; USDA National Institute of Food and Agriculture, Hatch project [OHO01279, OHO01303] FX Salaries and research support for this project were provided by the United Soybean Board, the Ohio Soybean Council, The Ohio State University Center for Applied Plant Sciences, and the USDA National Institute of Food and Agriculture, Hatch projects OHO01279 and OHO01303. NR 115 TC 1 Z9 1 U1 13 U2 13 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 11 PY 2016 VL 17 AR 607 DI 10.1186/s12864-016-2918-5 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DX0JD UT WOS:000384046700001 PM 27515508 ER PT J AU Hernandez-Castellano, LE Ferreira, AM Nanni, P Grossmann, J Arguello, A Capote, J Cai, GH Lippolis, J Castro, N de Almeida, AM AF Hernandez-Castellano, Lorenzo E. Ferreira, Ana M. Nanni, Paolo Grossmann, Jonas Arguello, Anastasio Capote, Juan Cai, Guohong Lippolis, John Castro, Noemi de Almeida, Andre M. TI The goat (Capra hircus) mammary gland secretory tissue proteome as influenced by weight loss: A study using label free proteomics SO JOURNAL OF PROTEOMICS LA English DT Article DE Goat; Mammary gland; Label-free proteomics; Seasonal weight loss ID TANDEM MASS-SPECTROMETRY; AUSTRALIAN MERINO LAMBS; WINTER VELD HAY; MILK-PRODUCTION; DAIRY-COWS; GENETIC-RESOURCES; ANIMAL PRODUCTION; FEED RESTRICTION; EARLY LACTATION; GUINEA-BISSAU AB Seasonal weight loss (SWL) is a significant limitation to animal production. Breeds that have evolved in harsh climates have acquired tolerance to SWL through selection. Herein, label free proteomics was used to characterize the effects of SWL in two goat breeds with different levels of adaptation to nutritional stress: Majorera (tolerant) and Palmera (susceptible). Nine Majorera and 10 Palmera dairy goats were used, divided in 4 sets, 2 for each breed: underfed and a control group: Majorera Control (MC), Palmera Control (PC), Majorera Restricted (ME) and Palmera Restricted (PE). At day 22, mammary gland biopsy samples were obtained. Label free proteomic analysis enabled the identification of over 1000 proteins, of which 96 showed differential expression between two of the groups within studied comparisons. Majorera breed showed higher expression of immune system related proteins. In contrast, Palmera breed showed higher expression of proteins related to apoptosis. Results indicate that the two goat breeds have a distinct metabolism reaction to SWL, and that proteins related to the immune system and apoptosis such as cadherin-13, collagen alpha-1, nidogen-2, clusterin and protein s100-A8 could be considered putative candidates as markers of tolerance to SWL. Biological significance: Seasonal weight loss (SWL) is one of the major constraints to animal production in the tropics. We compared the proteomics profiles of two dairy goat breeds with different levels of tolerance to SWL under control and feed restriction conditions using label free proteomics. We have identified over 1000 proteins in the goat mammary gland, of which 96 showed differential expression. Despite the fact that both breeds showed a decrease in the number of proteins related to protein, carbohydrates and fat biosynthesis, the tolerant breed showed higher expression of immune system related proteins compared to the susceptible breed. On the contrary, the susceptible breed had higher expression of apoptosis related proteins indicating that both breeds reacted differently to weight loss and that proteins related to the immune system and apoptosis such as cadherin-13, collagen alpha 1, nidogen-2 or clusterin may be suggested as markers of tolerance to SWL. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hernandez-Castellano, Lorenzo E.] Univ Bern, Vetsuisse Fac, Vet Physiol, Bremgartenstr 109a, CH-3001 Bern, Switzerland. [Ferreira, Ana M.; de Almeida, Andre M.] ITQB UNL Inst Tecnol Quim & Biol, P-2780 Oeiras, Portugal. [Ferreira, Ana M.] ICAAM Inst Ciencias Agr & Ambientais Mediterran, P-7006 Evora, Portugal. [Nanni, Paolo; Grossmann, Jonas] Univ ETH Zurich, Funct Genom Ctr Zurich, Winterthurerstr 190, CH-8057 Zurich, Switzerland. [Arguello, Anastasio; Castro, Noemi] Univ Las Palmas Gran Canaria, Dept Anim Sci, Arucas 35413, Gran Canaria, Spain. [Capote, Juan] ICIA Inst Canario Invest Agr, Tenerife 38270, Spain. [Cai, Guohong; Lippolis, John] USDA, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. [de Almeida, Andre M.] IBET Inst Biol Expt & Tecnol, P-2780 Oeiras, Portugal. [de Almeida, Andre M.] Ross Univ, Sch Vet Med, POB 334, Basseterre, St Kitts & Nevi. RP de Almeida, AM (reprint author), CVZ FMV, Av Univ Tecn, P-1300477 Lisbon, Portugal. EM aalmeida@fmv.utl.pt RI Arguello, Anastasio/B-4493-2010; Hernandez-Castellano, Lorenzo Enrique/T-1034-2016; Almeida, Andre/I-4369-2013 OI Arguello, Anastasio/0000-0002-4426-0678; Hernandez-Castellano, Lorenzo Enrique/0000-0003-2729-0434; Almeida, Andre/0000-0002-7810-3988 NR 70 TC 2 Z9 2 U1 3 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD AUG 11 PY 2016 VL 145 SI SI BP 60 EP 69 DI 10.1016/j.jprot.2016.03.030 PG 10 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA DW8UM UT WOS:000383931000007 PM 27017928 ER PT J AU Holmes, TRH Hain, CR Anderson, MC Crow, WT AF Holmes, Thomas R. H. Hain, Christopher R. Anderson, Martha C. Crow, Wade T. TI Cloud tolerance of remote-sensing technologies to measure land surface temperature SO HYDROLOGY AND EARTH SYSTEM SCIENCES LA English DT Article ID IN-SITU MEASUREMENTS; MICROWAVE OBSERVATIONS; BRIGHTNESS TEMPERATURE; WATER-VAPOR; VALIDATION; MSG/SEVIRI; CYCLE AB Conventional methods to estimate land surface temperature (LST) from space rely on the thermal infrared (TIR) spectral window and is limited to cloud-free scenes. To also provide LST estimates during periods with clouds, a new method was developed to estimate LST based on passive-microwave (MW) observations. The MW-LST product is informed by six polar-orbiting satellites to create a global record with up to eight observations per day for each 0.25 degrees resolution grid box. For days with sufficient observations, a continuous diurnal temperature cycle (DTC) was fitted. The main characteristics of the DTC were scaled to match those of a geostationary TIR-LST product. This paper tests the cloud tolerance of the MW-LST product. In particular, we demonstrate its stable performance with respect to flux tower observation sites (four in Europe and nine in the United States), over a range of cloudiness conditions up to heavily overcast skies. The results show that TIR-based LST has slightly better performance than MW-LST for clear-sky observations but suffers an increasing negative bias as cloud cover increases. This negative bias is caused by incomplete masking of cloud-covered areas within the TIR scene that affects many applications of TIR-LST. In contrast, for MW-LST we find no direct impact of clouds on its accuracy and bias. MW-LST can therefore be used to improve TIR cloud screening. Moreover, the ability to provide LST estimates for cloud-covered surfaces can help expand current clear-sky-only satellite retrieval products to all-weather applications. C1 [Holmes, Thomas R. H.; Anderson, Martha C.; Crow, Wade T.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Holmes, Thomas R. H.] NASA, Hydrol Sci Lab, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Hain, Christopher R.] Univ Maryland, Earth Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Holmes, TRH (reprint author), USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA.; Holmes, TRH (reprint author), NASA, Hydrol Sci Lab, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. EM thomas.r.holmes@nasa.gov RI Anderson, Martha/C-1720-2015 OI Anderson, Martha/0000-0003-0748-5525 FU NASA through the research grant "The Science of Terra and Aqua" [13-TERAQ13-0181] FX This work was funded by NASA through the research grant "The Science of Terra and Aqua" (13-TERAQ13-0181). We would further like to thank Li Fang (NOAA) for preparation and interpretation of GOES LST. NR 24 TC 0 Z9 0 U1 11 U2 11 PU COPERNICUS GESELLSCHAFT MBH PI GOTTINGEN PA BAHNHOFSALLEE 1E, GOTTINGEN, 37081, GERMANY SN 1027-5606 EI 1607-7938 J9 HYDROL EARTH SYST SC JI Hydrol. Earth Syst. Sci. PD AUG 11 PY 2016 VL 20 IS 8 BP 3263 EP 3275 DI 10.5194/hess-20-3263-2016 PG 13 WC Geosciences, Multidisciplinary; Water Resources SC Geology; Water Resources GA DV8IY UT WOS:000383181900001 ER PT J AU Thormann, I Reeves, P Reilley, A Engels, JMM Lohwasser, U Borner, A Pillen, K Richards, CM AF Thormann, Imke Reeves, Patrick Reilley, Ann Engels, Johannes M. M. Lohwasser, Ulrike Boerner, Andreas Pillen, Klaus Richards, Christopher M. TI Geography of Genetic Structure in Barley Wild Relative Hordeum vulgare subsp spontaneum in Jordan SO PLOS ONE LA English DT Article ID AB-QTL ANALYSIS; MULTILOCUS GENOTYPE DATA; SSP SPONTANEUM; POPULATION-STRUCTURE; FERTILE CRESCENT; RESISTANCE GENES; POWDERY MILDEW; SPRING BARLEY; EX-SITU; ENVIRONMENTAL ASSOCIATIONS AB Informed collecting, conservation, monitoring and utilization of genetic diversity requires knowledge of the distribution and structure of the variation occurring in a species. Hordeum vulgare subsp. spontaneum (K. Koch) Thell., a primary wild relative of barley, is an important source of genetic diversity for barley improvement and co-occurs with the domesticate within the center of origin. We studied the current distribution of genetic diversity and population structure in H. vulgare subsp. spontaneum in Jordan and investigated whether it is correlated with either spatial or climatic variation inferred from publically available climate layers commonly used in conservation and ecogeographical studies. The genetic structure of 32 populations collected in 2012 was analyzed with 37 SSRs. Three distinct genetic clusters were identified. Populations were characterized by admixture and high allelic richness, and genetic diversity was concentrated in the northern part of the study area. Genetic structure, spatial location and climate were not correlated. This may point out a limitation in using large scale climatic data layers to predict genetic diversity, especially as it is applied to regional genetic resources collections in H. vulgare subsp. spontaneum. C1 [Thormann, Imke; Engels, Johannes M. M.] Biovers Int, Rome, Italy. [Reeves, Patrick; Reilley, Ann; Richards, Christopher M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80522 USA. [Lohwasser, Ulrike; Boerner, Andreas] Leibniz Inst Plant Genet & Crop Plant Res, Genebank Dept, Gatersleben, Germany. [Pillen, Klaus] Univ Halle Wittenberg, Plant Breeding, Inst Agr & Nutr Sci, Halle, Germany. RP Thormann, I (reprint author), Biovers Int, Rome, Italy. EM i.thormann@cgiar.org RI Pillen, Klaus/I-6511-2012; OI Pillen, Klaus/0000-0003-4646-6351; Thormann, Imke/0000-0003-2703-9805; Reeves, Patrick/0000-0001-9991-1397 NR 110 TC 0 Z9 0 U1 9 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 11 PY 2016 VL 11 IS 8 AR e0160745 DI 10.1371/journal.pone.0160745 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3KY UT WOS:000381381100064 PM 27513459 ER PT J AU Xie, GY Olson, DH Blaustein, AR AF Xie, Gisselle Yang Olson, Deanna H. Blaustein, Andrew R. TI Projecting the Global Distribution of the Emerging Amphibian Fungal Pathogen, Batrachochytrium dendrobatidis, Based on IPCC Climate Futures SO PLOS ONE LA English DT Article ID CHYTRID FUNGUS; POPULATION DECLINES; RANAVIRUS INFECTION; WESTERN-HEMISPHERE; RANA-CATESBEIANA; DISEASE; CHYTRIDIOMYCOSIS; TEMPERATURE; ECOLOGY; EXTINCTIONS AB Projected changes in climate conditions are emerging as significant risk factors to numerous species, affecting habitat conditions and community interactions. Projections suggest species range shifts in response to climate change modifying environmental suitability and is supported by observational evidence. Both pathogens and their hosts can shift ranges with climate change. We consider how climate change may influence the distribution of the emerging infectious amphibian chytrid fungus, Batrachochytrium dendrobatidis (Bd), a pathogen associated with worldwide amphibian population losses. Using an expanded global Bd database and a novel modeling approach, we examined a broad set of climate metrics to model the Bd-climate niche globally and regionally, then project how climate change may influence Bd distributions. Previous research showed that Bd distribution is dependent on climatic variables, in particular temperature. We trained a machine-learning model (random forest) with the most comprehensive global compilation of Bd sampling records (similar to 5,000 site-level records, mid-2014 summary), including 13 climatic variables. We projected future Bd environmental suitability under IPCC scenarios. The learning model was trained with combined worldwide data (non-region specific) and also separately per region (region-specific). One goal of our study was to estimate of how Bd spatial risks may change under climate change based on the best available data. Our models supported differences in Bd-climate relationships among geographic regions. We projected that Bd ranges will shift into higher latitudes and altitudes due to increased environmental suitability in those regions under predicted climate change. Specifically, our model showed a broad expansion of areas environmentally suitable for establishment of Bd on amphibian hosts in the temperate zones of the Northern Hemisphere. Our projections are useful for the development of monitoring designs in these areas, especially for sensitive species and those vulnerable to multiple threats. C1 [Xie, Gisselle Yang; Blaustein, Andrew R.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Olson, Deanna H.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Olson, DH (reprint author), US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. EM dedeolson@fs.fed.us FU Oregon State University; U.S. Forest Service, Pacific Northwest Research Station FX This work was supported by Oregon State University, to GYX and ARB and the U.S. Forest Service, Pacific Northwest Research Station to DHO and GYX. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 86 TC 1 Z9 1 U1 43 U2 44 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 11 PY 2016 VL 11 IS 8 AR e0160746 DI 10.1371/journal.pone.0160746 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3KY UT WOS:000381381100065 PM 27513565 ER PT J AU De Palma, A Abrahamczyk, S Aizen, MA Albrecht, M Basset, Y Bates, A Blake, RJ Boutin, C Bugter, R Connop, S Cruz-Lopez, L Cunningham, SA Darvill, B Diekotter, T Dorn, S Downing, N Entling, MH Farwig, N Felicioli, A Fonte, SJ Fowler, R Franzen, M Goulson, D Grass, I Hanley, ME Hendrix, SD Herrmann, F Herzog, F Holzschuh, A Jauker, B Kessler, M Knight, ME Kruess, A Lavelle, P Le Feon, V Lentini, P Malone, LA Marshall, J Pachon, EM McFrederick, QS Morales, CL Mudri-Stojnic, S Nates-Parra, G Nilsson, SG Ockinger, E Osgathorpe, L Parra, A Peres, CA Persson, AS Petanidou, T Poveda, K Power, EF Quaranta, M Quintero, C Rader, R Richards, MH Roulston, T Rousseau, L Sadler, JP Samnegard, U Schellhorn, NA Schuepp, C Schweiger, O Smith-Pardo, AH Steffan-Dewenter, I Stout, JC Tonietto, RK Tscharntke, T Tylianakis, JM Verboven, HAF Vergara, CH Verhulst, J Westphal, C Yoon, HJ Purvis, A AF De Palma, Adriana Abrahamczyk, Stefan Aizen, Marcelo A. Albrecht, Matthias Basset, Yves Bates, Adam Blake, Robin J. Boutin, Celine Bugter, Rob Connop, Stuart Cruz-Lopez, Leopoldo Cunningham, Saul A. Darvill, Ben Diekoetter, Tim Dorn, Silvia Downing, Nicola Entling, Martin H. Farwig, Nina Felicioli, Antonio Fonte, Steven J. Fowler, Robert Franzen, Markus Goulson, Dave Grass, Ingo Hanley, Mick E. Hendrix, Stephen D. Herrmann, Farina Herzog, Felix Holzschuh, Andrea Jauker, Birgit Kessler, Michael Knight, M. E. Kruess, Andreas Lavelle, Patrick Le Feon, Violette Lentini, Pia Malone, Louise A. Marshall, Jon Pachon, Eliana Martinez McFrederick, Quinn S. Morales, Carolina L. Mudri-Stojnic, Sonja Nates-Parra, Guiomar Nilsson, Sven G. Ockinger, Erik Osgathorpe, Lynne Parra-H, Alejandro Peres, Carlos A. Persson, Anna S. Petanidou, Theodora Poveda, Katja Power, Eileen F. Quaranta, Marino Quintero, Carolina Rader, Romina Richards, Miriam H. Roulston, T'ai Rousseau, Laurent Sadler, Jonathan P. Samnegard, Ulrika Schellhorn, Nancy A. Schuepp, Christof Schweiger, Oliver Smith-Pardo, Allan H. Steffan-Dewenter, Ingolf Stout, Jane C. Tonietto, Rebecca K. Tscharntke, Teja Tylianakis, Jason M. Verboven, Hans A. F. Vergara, Carlos H. Verhulst, Jort Westphal, Catrin Yoon, Hyung Joo Purvis, Andy TI Predicting bee community responses to land-use changes: Effects of geographic and taxonomic biases SO SCIENTIFIC REPORTS LA English DT Article ID BOMBUS SPP. HYMENOPTERA; AGRICULTURAL LANDSCAPES; SPECIES RICHNESS; POLLINATOR DECLINES; DIVERSITY HYMENOPTERA; SPATIAL-DISTRIBUTION; USE INTENSIFICATION; ECOLOGICAL TRAITS; WILD POLLINATORS; FORAGING RANGE AB Land-use change and intensification threaten bee populations worldwide, imperilling pollination services. Global models are needed to better characterise, project, and mitigate bees' responses to these human impacts. The available data are, however, geographically and taxonomically unrepresentative; most data are from North America and Western Europe, overrepresenting bumblebees and raising concerns that model results may not be generalizable to other regions and taxa. To assess whether the geographic and taxonomic biases of data could undermine effectiveness of models for conservation policy, we have collated from the published literature a global dataset of bee diversity at sites facing land-use change and intensification, and assess whether bee responses to these pressures vary across 11 regions (Western, Northern, Eastern and Southern Europe; North, Central and South America; Australia and New Zealand; South East Asia; Middle and Southern Africa) and between bumblebees and other bees. Our analyses highlight strong regionally-based responses of total abundance, species richness and Simpson's diversity to land use, caused by variation in the sensitivity of species and potentially in the nature of threats. These results suggest that global extrapolation of models based on geographically and taxonomically restricted data may underestimate the true uncertainty, increasing the risk of ecological surprises. C1 [De Palma, Adriana; Tylianakis, Jason M.; Purvis, Andy] Imperial Coll London, Dept Life Sci, Silwood Pk Campus,Buckhurst Rd, Ascot SL5 7PY, Berks, England. [De Palma, Adriana; Purvis, Andy] Nat Hist Museum, Dept Life Sci, Cromwell Rd, London SW7 5BD, England. [Abrahamczyk, Stefan] Univ Bonn, Nees Inst Plant Biodivers, Meckenheimer Allee 170, D-53115 Bonn, Germany. [Aizen, Marcelo A.; Morales, Carolina L.; Quintero, Carolina] Univ Nacl Comahue, CONICET, INIBIOMA, Lab Ecotono, Quintral 1250, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. [Albrecht, Matthias] Agroscope, Inst Sustainabil Sci, Reckenholzstr 191, CH-8046 Zurich, Switzerland. [Basset, Yves] Smithsonian Trop Res Inst, Apartado 0843-03092, Panama City, Panama. [Bates, Adam] Nottingham Trent Univ, Biosci, Nottingham NG11 8NS, England. [Blake, Robin J.] Univ Reading, Sch Agr Policy & Dev, Ctr Agri Environm Res, Reading RG6 6AR, Berks, England. [Boutin, Celine] Carleton Univ, Sci & Technol Branch, Environm & Climate Change Canada, 1125 Colonel By Dr, Ottawa, ON K1A 0H3, Canada. [Bugter, Rob] Part Wageningen Univ & Res, Alterra, POB 47, NL-6700 AA Wageningen, Netherlands. [Connop, Stuart] Univ East London, Sustainabil Res Inst, 4-6 Univ Way, London E16 2RD, England. [Cruz-Lopez, Leopoldo] El Colegio Frontera ECOSUR, Grp Ecol & Manejo Artropodos, Carretera Antiguo Aeropuerto Km 2-5, Chiapas 30700, Mexico. [Cunningham, Saul A.] CSIRO Land & Water, Canberra, ACT 2601, Australia. [Darvill, Ben] Univ Stirling, British Trust Ornithol Scotland Biol & Environm S, Stirling FK9 4LA, Scotland. [Diekoetter, Tim] Univ Kiel, Inst Nat Resource Conservat, Dept Landscape Ecol, Olshausenstr 75, D-24118 Kiel, Germany. [Diekoetter, Tim] Univ Marburg, Nat Conservat, Dept Biol, Marburg, Germany. [Diekoetter, Tim] Swiss Fed Inst Technol, Inst Integrat Biol, Zurich, Switzerland. [Dorn, Silvia] ETH, Appl Entomol, Schmelzbergstr 7-LFO, CH-8092 Zurich, Switzerland. [Downing, Nicola] RSPB, Scottish Headquarters 2 Lochside View, Edinburgh EH12 9DH, Midlothian, Scotland. [Entling, Martin H.] Univ Koblenz Landau, Inst Environm Sci, Fortstr 7, D-76829 Landau, Germany. [Farwig, Nina] Univ Marburg, Fac Biol, Conservat Ecol, Karl von Frisch Str 8, D-35032 Marburg, Germany. [Felicioli, Antonio] Univ Pisa, Dipartimento Sci Vet, Viale Piagge 2, I-56100 Pisa, Italy. [Fonte, Steven J.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Fowler, Robert; Goulson, Dave] Univ Sussex, Sch Life Sci, Brighton BN1 9QG, E Sussex, England. [Franzen, Markus; Schweiger, Oliver] UFZ Helmholtz Ctr Environm Res, Dept Community Ecol, Theodor Lieser Str 4, D-06120 Halle, Germany. [Grass, Ingo; Herrmann, Farina; Tscharntke, Teja; Westphal, Catrin] Univ Gottingen, Dept Crop Sci, Agroecol, D-37077 Gottingen, Germany. [Hanley, Mick E.; Knight, M. E.] Univ Plymouth, Sch Biol Sci, Plymouth PL4 8AA, Devon, England. [Hendrix, Stephen D.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. [Herzog, Felix] Agroscope, Inst Sustainabil Sci, CH-8046 Zurich, Switzerland. [Holzschuh, Andrea; Steffan-Dewenter, Ingolf] Univ Wurzburg, Bioctr, Dept Anim Ecol & Trop Biol, D-97074 Wurzburg, Germany. [Jauker, Birgit] Univ Giessen, Dept Anim Ecol, Heinrich Buff Ring 26-32, D-35392 Giessen, Germany. [Kessler, Michael] Inst Systemat & Evolutionare Bot, Zurich, Switzerland. [Kruess, Andreas] Fed Agcy Nat Conservat, BfN, Dept Ecol & Conservat Fauna & Flora, Konstantinstr 110, D-53179 Bonn, Germany. [Lavelle, Patrick] IRD, F-93143 Bondy, France. [Lavelle, Patrick] CIAT, Trop Soil Biol & Fertil Program, Latin Amer & Caribbean Reg, Cali, Colombia. [Le Feon, Violette] INRA, UR Abeilles & Environm 406, CS 40509, F-84914 Avignon, France. [Lentini, Pia] Univ Melbourne, Sch BioSci, Parkville, Vic 3010, Australia. [Malone, Louise A.] New Zealand Inst Plant & Food Res Ltd, Auckland Mail Ctr, Private Bag 92169, Auckland 1142, New Zealand. [Marshall, Jon] Marshall Agroecol Ltd, 2 Nut Tree Cottages, Barton BS25 1DU, Winscombe, England. [Pachon, Eliana Martinez; Nates-Parra, Guiomar] Univ Nacl Colombia, Fac Ciencias, Dept Biol, Bogota, Colombia. [McFrederick, Quinn S.] Univ Calif Riverside, Dept Entomol, 900 Univ Ave, Riverside, CA 92521 USA. [Mudri-Stojnic, Sonja] Univ Novi Sad, Fac Sci, Dept Biol & Ecol, Novi Sad 21000, Serbia. [Nilsson, Sven G.; Persson, Anna S.] Lund Univ, Dept Biol, SE-22362 Lund, Sweden. [Ockinger, Erik] Swedish Univ Agr Sci, Dept Ecol, Box 7044, SE-75007 Uppsala, Sweden. [Osgathorpe, Lynne] RSPB, UK Headquarters Lodge, Sandy, Beds, England. [Parra-H, Alejandro] Univ Nacl Colombia, Fac Ciencias, Dept Biol, Lab Invest Abejas,LABUN, Carrera 45 26-85,Edif Uriel Gutierrez, Bogota, Colombia. [Parra-H, Alejandro] Corp Gest Serv Ecosistem Polinizac & Abejas SEPyA, Bogota, Colombia. [Peres, Carlos A.] Univ East Anglia, Sch Environm Sci, Norwich NR4 7TJ, Norfolk, England. [Petanidou, Theodora] Univ Aegean, Dept Geog, Lab Biogeog & Ecol, Mitilini 81100, Greece. [Poveda, Katja] Cornell Univ, Dept Entomol, Ithaca, NY 14850 USA. [Power, Eileen F.; Stout, Jane C.] Trinity Coll Dublin, Sch Nat Sci, Bot, Dublin 2, Ireland. [Quaranta, Marino] Consiglio Ric Agr Anal Econ Agr, CREA ABP, Ctr Ric Agrobiol & Pedol, Via Lanciola 12-A, I-50125 Florence, Italy. [Rader, Romina] Univ New England, Sch Environm & Rural Sci, Armidale, NSW, Australia. [Richards, Miriam H.] Brock Univ, Dept Biol Sci, St Catharines, ON L2S 3A1, Canada. [Roulston, T'ai] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22904 USA. [Roulston, T'ai] Blandy Expt Farm, 400 Blandy Farm Lane, Boyce, VA 22620 USA. [Rousseau, Laurent] Univ Quebec, Dept Sci Biol, CP 8888,Succursale Ctr Ville, Montreal, PQ H3C 3P8, Canada. [Sadler, Jonathan P.] Univ Birmingham, GEES Sch Geog Earth & Environm Sci, Birmingham B15 2TT, W Midlands, England. [Samnegard, Ulrika] Stockholm Univ, Dept Ecol Environm & Plant Sci, SE-10691 Stockholm, Sweden. [Schellhorn, Nancy A.] CSIRO, Dutton Pk, Qld 4102, Australia. [Schuepp, Christof] Univ Bern, Inst Ecol & Evolut Community Ecol, Baltzerstr 6, CH-3012 Bern, Switzerland. [Smith-Pardo, Allan H.] USDA, Anim & Plant Hlth Inspect Serv, Plant Protect & Quarantine, San Francisco, CA 94080 USA. [Smith-Pardo, Allan H.] Univ Nacl Colombia, Medellin UNALMED, Fac Sci, Bogota, Colombia. [Tonietto, Rebecca K.] Northwestern Univ, Plant Biol & Conservat, 2205 Tech Dr,OT Hogan Hall Rm 2-1444, Evanston, IL 60208 USA. [Tonietto, Rebecca K.] Chicago Bot Garden, 1000 Lake Cook Rd, Glencoe, IL 60011 USA. [Tonietto, Rebecca K.] St Louis Univ, Dept Bot, 3507 Laclede Ave,Macelwane Hall, St Louis, MO 63103 USA. [Tylianakis, Jason M.] Univ Canterbury, Sch Biol Sci, Ctr Integrat Ecol, Private Bag 4800, Christchurch 8140, New Zealand. [Verboven, Hans A. F.] Katholieke Univ Leuven, Dept Earth & Environm Sci, Div Forest Nat & Landscape, Celestijnenlaan 200E, B-3001 Leuven, Belgium. [Vergara, Carlos H.] Univ Americas Puebla, Dept Ciencias Quim Biol, Cholula, Pue, Mexico. [Verhulst, Jort] Spotvogellaan 68, NL-2566 PN The Hague, Netherlands. [Yoon, Hyung Joo] Natl Inst Agr Sci, RDA, Dept Agr Biol, Wanju Gun 55365, Jellabuk Do, South Korea. RP De Palma, A (reprint author), Imperial Coll London, Dept Life Sci, Silwood Pk Campus,Buckhurst Rd, Ascot SL5 7PY, Berks, England. EM adrid@nhm.ac.uk RI Purvis, Andy/A-7529-2008; Herzog, Felix/B-1911-2009; Schellhorn, Nancy/B-6716-2009; Entling, Martin/C-6953-2008; Kessler, Michael/A-3605-2009; Peres, Carlos/B-1276-2013; Cunningham, Saul/B-9947-2009; Schweiger, Oliver/B-4909-2008; Jauker, Birgit/A-6281-2009 OI Aizen, Marcelo/0000-0001-9079-9749; Lentini, Pia/0000-0002-3520-3460; Purvis, Andy/0000-0002-8609-6204; Fowler, Robert/0000-0002-5246-8831; Peres, Carlos/0000-0002-1588-8765; Cunningham, Saul/0000-0003-0703-6893; Schweiger, Oliver/0000-0001-8779-2335; Quaranta, Marino/0000-0003-0082-4555; De Palma, Adriana/0000-0002-5345-4917; Jauker, Birgit/0000-0001-5027-9351 FU BBSRC [BB/F017324/1]; NERC [NE/J011193/1, NE/M014533/1] FX We are grateful to all members of the PREDICTS team who aided in the collation and curation of data. We are especially grateful to all the many researchers who have made their data available to us. This paper is a contribution from the Imperial College Grand Challenges in Ecosystems and the Environment Initiative. The PREDICTS project is endorsed by the GEO BON. We thank BBSRC (grant BB/F017324/1 to ADP) and NERC (grant NE/J011193/1 and NE/M014533/1 to AP) for support. NR 133 TC 2 Z9 2 U1 72 U2 91 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD AUG 11 PY 2016 VL 6 AR 31153 DI 10.1038/srep31153 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT0VL UT WOS:000381201000001 PM 27509831 ER PT J AU Morelli, TL Daly, C Dobrowski, SZ Dulen, DM Ebersole, JL Jackson, ST Lundquist, JD Millar, CI Maher, SP Monahan, WB Nydick, KR Redmond, KT Sawyer, SC Stock, S Beissinger, SR AF Morelli, Toni Lyn Daly, Christopher Dobrowski, Solomon Z. Dulen, Deanna M. Ebersole, Joseph L. Jackson, Stephen T. Lundquist, Jessica D. Millar, Constance I. Maher, Sean P. Monahan, William B. Nydick, Koren R. Redmond, Kelly T. Sawyer, Sarah C. Stock, Sarah Beissinger, Steven R. TI Managing Climate Change Refugia for Climate Adaptation SO PLOS ONE LA English DT Article ID UNITED-STATES; RIVER RESTORATION; CHANGE IMPACTS; BIODIVERSITY; MICROREFUGIA; ECOSYSTEMS; MODELS; CONSERVATION; COMMUNITIES; RESPONSES AB Refugia have long been studied from paleontological and biogeographical perspectives to understand how populations persisted during past periods of unfavorable climate. Recently, researchers have applied the idea to contemporary landscapes to identify climate change refugia, here defined as areas relatively buffered from contemporary climate change over time that enable persistence of valued physical, ecological, and socio-cultural resources. We differentiate historical and contemporary views, and characterize physical and ecological processes that create and maintain climate change refugia. We then delineate how refugia can fit into existing decision support frameworks for climate adaptation and describe seven steps for managing them. Finally, we identify challenges and opportunities for operationalizing the concept of climate change refugia. Managing climate change refugia can be an important option for conservation in the face of ongoing climate change. C1 [Morelli, Toni Lyn] US Geol Survey, DOI Northeast Climate Sci Ctr, Amherst, MA 01002 USA. [Morelli, Toni Lyn; Maher, Sean P.; Beissinger, Steven R.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Morelli, Toni Lyn; Maher, Sean P.; Beissinger, Steven R.] Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA. [Daly, Christopher] Oregon State Univ, Coll Engn, Corvallis, OR 97331 USA. [Dobrowski, Solomon Z.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Dulen, Deanna M.] Natl Pk Serv, Devils Postpile Natl Monument, Mammoth Lakes, CA 93546 USA. [Ebersole, Joseph L.] US EPA, Western Ecol Div, Corvallis, OR 97333 USA. [Jackson, Stephen T.] US Geol Survey, DOI Southwest Climate Sci Ctr, Tucson, AZ 85721 USA. [Jackson, Stephen T.] Univ Arizona, Dept Geosci, Tucson, AZ 85721 USA. [Jackson, Stephen T.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Lundquist, Jessica D.] Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA. [Millar, Constance I.] USDA Forest Serv, Pacific Southwest Res Stn, Albany, CA 94710 USA. [Maher, Sean P.] Missouri State Univ, Dept Biol, Springfield, MO 65897 USA. [Monahan, William B.] USDA Forest Serv, Forest Hlth Technol Enterprise Team, Ft Collins, CO 80526 USA. [Nydick, Koren R.] Natl Pk Serv, Sequoia & Kings Canyon Natl Pk, Three Rivers, CA USA. [Redmond, Kelly T.] Desert Res Inst, Western Reg Climate Ctr, Reno, NV 89506 USA. [Sawyer, Sarah C.] USDA Forest Serv, Pacific Southwest Reg, Vallejo, CA 94592 USA. [Stock, Sarah] Natl Pk Serv, Yosemite Natl Pk, El Portal, CA 95318 USA. RP Morelli, TL (reprint author), US Geol Survey, DOI Northeast Climate Sci Ctr, Amherst, MA 01002 USA.; Morelli, TL (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA.; Morelli, TL (reprint author), Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA. EM tmorelli@usgs.gov OI Morelli, Toni Lyn/0000-0001-5865-5294 FU California Landscape Conservation Cooperative; Department of Interior Northeast Climate Science Center FX This work was supported by funding from the California Landscape Conservation Cooperative (www.californialcc.org) and the Department of Interior Northeast Climate Science Center (necsc.umass.edu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 76 TC 5 Z9 5 U1 27 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 10 PY 2016 VL 11 IS 8 AR e0159909 DI 10.1371/journal.pone.0159909 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3KR UT WOS:000381380400017 PM 27509088 ER PT J AU Xiong, HZ Shi, AN Mou, BQ Qin, J Motes, D Lu, WG Ma, JB Weng, YJ Yang, W Wu, DX AF Xiong, Haizheng Shi, Ainong Mou, Beiquan Qin, Jun Motes, Dennis Lu, Weiguo Ma, Jianbing Weng, Yuejin Yang, Wei Wu, Dianxing TI Genetic Diversity and Population Structure of Cowpea (Vigna unguiculata L. Walp) SO PLOS ONE LA English DT Article ID GENUS VIGNA; LINKAGE DISEQUILIBRIUM; AFLP ANALYSIS; SSR MARKERS; GERMPLASM; SNP; WILD; DNA; ASSOCIATION; ACCESSIONS AB The genetic diversity of cowpea was analyzed, and the population structure was estimated in a diverse set of 768 cultivated cowpea genotypes from the USDA GRIN cowpea collection, originally collected from 56 countries. Genotyping by sequencing was used to discover single nucleotide polymorphism (SNP) in cowpea and the identified SNP alleles were used to estimate the level of genetic diversity, population structure, and phylogenetic relationships. The aim of this study was to detect the gene pool structure of cowpea and to determine its relationship between different regions and countries. Based on the model-based ancestry analysis, the phylogenetic tree, and the principal component analysis, three well-differentiated genetic populations were postulated from 768 worldwide cowpea genotypes. According to the phylogenetic analyses between each individual, region, and country, we may trace the accession from off-original, back to the two candidate original areas (West and East of Africa) to predict the migration and domestication history during the cowpea dispersal and development. To our knowledge, this is the first report of the analysis of the genetic variation and relationship between globally cultivated cowpea genotypes. The results will help curators, researchers, and breeders to understand, utilize, conserve, and manage the collection for more efficient contribution to international cowpea research. C1 [Xiong, Haizheng; Shi, Ainong; Qin, Jun; Lu, Weiguo; Ma, Jianbing; Weng, Yuejin; Yang, Wei] Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA. [Mou, Beiquan] ARS, USDA, Salinas, CA 93905 USA. [Motes, Dennis] Univ Arkansas, Vegetable Res Ctr, Alma, AR USA. [Xiong, Haizheng; Wu, Dianxing] Zhejiang Univ, State Key Lab Rice Biol, IAEA Collaborating Ctr, Hangzhou, Zhejiang, Peoples R China. RP Shi, AN (reprint author), Univ Arkansas, Dept Hort, Fayetteville, AR 72701 USA.; Mou, BQ (reprint author), ARS, USDA, Salinas, CA 93905 USA. EM ashi@uark.edu; Beiquan.Mou@ars.usda.gov FU USDA National Institute of Food and Agriculture Hatch project [1002423] FX USDA National Institute of Food and Agriculture Hatch project accession number 1002423.; Cowpea germplasm accessions were provided by USDA-ARS Station at GRIFFIN, GA. The cowpea_Genome_0.03.fa (6,750 scaffolds or contigs) (http://harvest-blast.org/) was kindly provided by Dr. Timothy J. Close, University of California, U.S.A. This work is supported, at least in part, by the USDA National Institute of Food and Agriculture Hatch project accession number 1002423. NR 48 TC 2 Z9 2 U1 15 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 10 PY 2016 VL 11 IS 8 AR e0160941 DI 10.1371/journal.pone.0160941 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3KR UT WOS:000381380400070 ER PT J AU Edwards, JD Baldo, AM Mueller, LA AF Edwards, J. D. Baldo, A. M. Mueller, L. A. TI Ricebase: a breeding and genetics platform for rice, integrating individual molecular markers, pedigrees and whole-genome-based data SO DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION LA English DT Article ID ORYZA-SATIVA L.; DRAFT SEQUENCE; RESOURCES AB Ricebase (http://ricebase.org) is an integrative genomic database for rice (Oryza sativa) with an emphasis on combining datasets in a way that maintains the key links between past and current genetic studies. Ricebase includes DNA sequence data, gene annotations, nucleotide variation data and molecular marker fragment size data. Rice research has benefited from early adoption and extensive use of simple sequence repeat (SSR) markers; however, the majority of rice SSR markers were developed prior to the latest rice pseudomolecule assembly. Interpretation of new research using SNPs in the context of literature citing SSRs requires a common coordinate system. A new pipeline, using a stepwise relaxation of stringency, was used to map SSR primers onto the latest rice pseudomolecule assembly. The SSR markers and experimentally assayed amplicon sizes are presented in a relational database with a web-based front end, and are available as a track loaded in a genome browser with links connecting the browser and database. The combined capabilities of Ricebase link genetic markers, genome context, allele states across rice germplasm and potentially user curated phenotypic interpretations as a community resource for genetic discovery and breeding in rice. C1 [Edwards, J. D.; Baldo, A. M.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Mueller, L. A.] Boyce Thompson Inst Plant Res, Ithaca, NY USA. RP Edwards, JD (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM jeremy.edwards@ars.usda.gov NR 23 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1758-0463 J9 DATABASE-OXFORD JI Database PD AUG 10 PY 2016 AR baw107 DI 10.1093/database/baw107 PG 6 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA DT5KK UT WOS:000381521900001 ER PT J AU Zhang, ZH Feng, MY Zhu, KC Han, LJ Sapozhnikova, Y Lehotay, SJ AF Zhang, Zihao Feng, Mengyuan Zhu, Kechen Han, Lijun Sapozhnikova, Yelena Lehotay, Steven J. TI Multiresidue Analysis of Pesticides in Straw Roughage by Liquid Chromatography-Tandem Mass Spectrometry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article; Proceedings Paper CT 52nd North American Chemical Residue Workshop (NACRW) CY JUL 19-22, 2015 CL Flag Works Inc, St Pete Beach, FL HO Flag Works Inc DE multiresidue pesticide analysis; rice and wheat straw; animal feed; QuEChERS; LC-MS/MS ID SOLID-PHASE EXTRACTION; PRESSURE GAS-CHROMATOGRAPHY; WALLED CARBON NANOTUBES; RESIDUE ANALYSIS; ANIMAL FEED; LC-MS/MS; QUECHERS; DISSIPATION; WHEAT; VEGETABLES AB A multiresidue analytical method using a modification of the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) sample preparation approach combined with liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis was established and validated for the rapid determination of 69 pesticides at different levels (1-100 ng/g) in wheat and rice straws. In the quantitative analysis, the recoveries ranged from 70 to 120%, and consistent RSDs <= 20% were achieved for most of the target analytes (53 pesticides in wheat straw and 58 in rice straw). Almost all of the analytes achieved good linearity with R-2 > 0.98, and the limit of validation levels (LVLs) for diverse pesticides ranged from 1 to 10 ng/g. Different extraction and cleanup conditions were evaluated in both types of straw, leading to different options. The use of 0.1% formic acid or not in extraction with acetonitrile yielded similar final outcomes, but led to the use of a different sorbent in dispersive solid-phase extraction. Both options are efficient and useful for the multiresidue analysis of targeted pesticides in wheat and rice straw samples. C1 [Zhang, Zihao; Feng, Mengyuan; Zhu, Kechen; Han, Lijun] China Agr Univ, Coll Sci, Beijing 100193, Peoples R China. [Sapozhnikova, Yelena; Lehotay, Steven J.] USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. RP Han, LJ (reprint author), China Agr Univ, Coll Sci, Beijing 100193, Peoples R China. EM hanlijun2000@163.com NR 36 TC 1 Z9 1 U1 5 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 10 PY 2016 VL 64 IS 31 BP 6091 EP 6099 DI 10.1021/acs.jafc.5b05981 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DT2TC UT WOS:000381332400005 PM 26881844 ER PT J AU Gardner, DR Cook, D AF Gardner, Dale R. Cook, Daniel TI Analysis of Swainsonine and Swainsonine N-Oxide as Trimethylsilyl Derivatives by Liquid Chromatography-Mass Spectrometry and Their Relative Occurrence in Plants Toxic to Livestock SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE swainsonine; swainsonine N-oxide; locoweeds; HPLC-MS; trimethylsilyl derivative ID ENDOPHYTIC UNDIFILUM FUNGI; ALKALOID SWAINSONINE; LOCOWEED; ASTRAGALUS; OXYTROPIS; IDENTIFICATION; EXTRACTION; CANESCENS; SPP.; SEED AB There are limited data concerning the occurrence of swainsonine N-oxide in plants known to contain swainsonine and its relative impact on toxicity of the plant material. A liquid chromatography mass spectrometry method based on a solvent partitioning extraction procedure followed by trimethylsilylation and analysis using reversed phase high-pressure liquid chromatography mass spectrometry was developed for the analysis of swainsonine and its N-oxide. The concentrations of each were measured in several swainsonine-containing taxa as well as two endophytic isolates that produce swainsonine. In vegetative samples the relative percent of N-oxide to free base ranged from 0.9 to 18%. In seed samples the N-oxide to free base ratio ranged from 0 to 10%. The measured concentrations of swainsonine N-oxide relative to swainsonine only slightly increases the actual toxicity of the various plant samples in a combined assay of both compounds. C1 [Gardner, Dale R.; Cook, Daniel] ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. RP Gardner, DR (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM dale.gardner@ars.usda.gov FU Utah State University; National Science Foundation [CHE-1429195] FX We thank Utah State University and the National Science Foundation (CHE-1429195) for use of and funding for the Bruker 500 MHz NMR. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 30 TC 0 Z9 0 U1 5 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 10 PY 2016 VL 64 IS 31 BP 6156 EP 6162 DI 10.1021/acs.jafc.6b02390 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DT2TC UT WOS:000381332400012 PM 27436221 ER PT J AU Romadanova, N Mishustina, S Karasholakova, L Reed, BM Kushnarenko, S AF Romadanova, Natalya Mishustina, Svetlana Karasholakova, Lazzat Reed, Barbara M. Kushnarenko, Svetlana TI In vitro collection of cultivars and wild forms of Berberis spp for cryobank creation SO JOURNAL OF BIOTECHNOLOGY LA English DT Meeting Abstract CT European Biotechnology Conference CY MAY 05-07, 2016 CL LATVIA C1 [Romadanova, Natalya; Mishustina, Svetlana; Karasholakova, Lazzat; Kushnarenko, Svetlana] Inst Plant Biol & Biotechnol, Alma Ata, Kazakhstan. [Reed, Barbara M.] ARS, USDA, Natl Clonal Germplasm Repository, Washington, DC USA. EM nata_romadanova@mail.ru NR 0 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1656 EI 1873-4863 J9 J BIOTECHNOL JI J. Biotechnol. PD AUG 10 PY 2016 VL 231 SU S BP S39 EP S39 DI 10.1016/j.jbiotec.2016.05.153 PG 1 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DR9TZ UT WOS:000380240300115 ER PT J AU Walters, JP Archer, DW Sassenrath, GF Hendrickson, JR Hanson, JD Halloran, JM Vadas, P Alarcon, VJ AF Walters, Jeffrey P. Archer, David W. Sassenrath, Gretchen F. Hendrickson, John R. Hanson, Jon D. Halloran, John M. Vadas, Peter Alarcon, Vladimir J. TI Exploring agricultural production systems and their fundamental components with system dynamics modelling SO ECOLOGICAL MODELLING LA English DT Article DE Sustainability; Agriculture production systems; System dynamics modelling; Drivers; Farming policy ID COMPLEX ECOLOGICAL-SYSTEMS; ECONOMIC-SYSTEMS; UNITED-STATES; STELLA; PRINCIPLES; VALIDATION; DRIVERS AB Agricultural production in the United States is undergoing marked changes due to rapid shifts in consumer demands, input costs, and concerns for food safety and environmental impact. Agricultural production systems are comprised of multidimensional components and drivers that interact in complex ways to influence production sustainability. In a mixed-methods approach, we combine qualitative and quantitative data to develop and simulate a system dynamics model that explores the systemic interaction of these drivers on the economic, environmental and social sustainability of agricultural production. We then use this model to evaluate the role of each driver in determining the differences in sustainability between three distinct production systems: crops only, livestock only, and an integrated crops and livestock system. The result from these modelling efforts found that the greatest potential for sustainability existed with the crops only production system. While this study presents a stand-alone contribution to sector knowledge and practice, it encourages future research in this sector that employs similar systems-based methods to enable more sustainable practices and policies within agricultural production. (C) 2016 The Authors. Published by Elsevier B.V. C1 [Walters, Jeffrey P.; Alarcon, Vladimir J.] Univ Diego Portales, Dept Civil Engn, Av Ejercito 441, Santiago, Chile. [Archer, David W.; Hendrickson, John R.; Hanson, Jon D.] USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. [Sassenrath, Gretchen F.] Kansas State Univ, Southeast Res & Extens Ctr, POB 316, Parsons, KS 67375 USA. [Halloran, John M.] USDA ARS, New England Plant Soil & Water Lab, Orono, ME USA. [Vadas, Peter] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Walters, JP (reprint author), Univ Diego Portales, Dept Civil Engn, Av Ejercito 441, Santiago, Chile. EM jeffrey.walters@udp.cl; david.archer@ars.usda.gov; gsassenrath@ksu.edu; john.hendrickson@ars.usda.gov; jon.hanson@npbsonline.org; johnhalloran75@gmail.com; peter.vadas@ars.usda.gov; vladimir.alarcon@udp.cl RI Alarcon, Vladimir/P-9202-2016; OI Alarcon, Vladimir/0000-0001-6629-5639; Walters, Jeffrey/0000-0001-6655-1233 FU CONICYT [REDES 140045] FX The authors would like to express our appreciation for the many farmers and ranchers who contributed to this study. This manuscript is contribution number 16-194-j from the Kansas Agricultural Experiment Station. This research was supported in part by a grant from CONICYT (REDES 140045). NR 52 TC 2 Z9 2 U1 26 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD AUG 10 PY 2016 VL 333 SI 1 BP 51 EP 65 DI 10.1016/j.ecolmodel.2016.04.015 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DN8EW UT WOS:000377313000004 ER PT J AU Clough, BJ Russell, MB Domke, GM Woodall, CW Radtke, PJ AF Clough, Brian J. Russell, Matthew B. Domke, Grant M. Woodall, Christopher W. Radtke, Philip J. TI Comparing tree foliage biomass models fitted to a multispecies, felled-tree biomass dataset for the United States SO ECOLOGICAL MODELLING LA English DT Article DE Foliage biomass models; Component ratio models; Bayesian hierarchical models; Posterior predictive checking; Prediction uncertainty ID LEAF-AREA INDEX; NORWAY SPRUCE; DOUGLAS-FIR; ABOVEGROUND BIOMASS; UNCERTAINTY ESTIMATION; ALLOMETRIC EQUATIONS; CENTRAL-EUROPE; BLACK SPRUCE; SAPWOOD AREA; PREDICTION AB Estimation of live tree biomass is an important task for both forest carbon accounting and studies of nutrient dynamics in forest ecosystems. In this study, we took advantage of an extensive felled-tree database (with 2885 foliage biomass observations) to compare different models and grouping schemes based on phylogenetic and geographic variation for predicting foliage biomass at the tree scale. We adopted a Bayesian hierarchical statistical framework, first to compare linear models that predict foliage biomass directly to models that separately estimate a foliage ratio as a component of total aboveground biomass, then to compare species specific models to both 'narrow' and 'broad' general biomass models using the best fitted functional form. We evaluated models by simulating new datasets from the posterior predictive distribution, using both summary statistics and visual assessments of model performance. Key findings of our study were: (1) simple linear models provided a better fit to our data than component ratio models, where total biomass and the foliar ratio are estimated separately; (2) species-specific equations provided the best predictive performance, and there was no advantage to narrow species groupings relative to broader groups; and (3) all three model schemes (i.e., species-specific models versus narrow or broad groupings proposed in national-scale biomass equations) tended to over-predict foliage biomass and resulted in predictions with very high uncertainty, particularly for large diameter trees. This analysis represents a fundamental shift in carbon accounting by employing felled-tree data to refine our understanding of uncertainty associated with component biomass estimates, and presents an ideal approach to account for tree-scale allometric model error when estimating forest carbon stocks. However, our results also highlight the need for substantial improvements to both available fitting data and models for foliage biomass before this approach is implemented within the context of greenhouse gas inventories. (C) 2016 Elsevier B.V. All rights reserved. C1 [Clough, Brian J.; Russell, Matthew B.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Domke, Grant M.; Woodall, Christopher W.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. [Radtke, Philip J.] Virginia Polytech Inst & State Univ, Forest Resources & Environm Conservat, Blacksburg, VA USA. RP Clough, BJ (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN USA. EM bclough@umn.edu OI Domke, Grant/0000-0003-0485-0355 FU U.S. Department of Agriculture, Forest Service, Northern Research Station; Minnesota Agricultural Experiment Station FX We wish to thank David Walker, Jereme Frank, Aaron Weiskittel, and all who have contributed to the U.S. Forest Service Volume Biomass Project and the Legacy database. In addition we would like to thank John Stanovick, David Bell, Kenneth Elgersma, and three anonymous reviewers for their comments on our manuscript. This research is funded by the U.S. Department of Agriculture, Forest Service, Northern Research Station and the Minnesota Agricultural Experiment Station. NR 119 TC 0 Z9 0 U1 7 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD AUG 10 PY 2016 VL 333 SI 1 BP 79 EP 91 DI 10.1016/j.ecolmodel.2016.04.009 PG 13 WC Ecology SC Environmental Sciences & Ecology GA DN8EW UT WOS:000377313000006 ER PT J AU Evenhuis, JP LaFrentz, BR AF Evenhuis, Jason P. LaFrentz, Benjamin R. TI Virulence of Flavobacterium columnare genomovars in rainbow trout Oncorhynchus mykiss SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Flavobacterium columnare; Rainbow trout; Genomovar; Virulence; Immersion challenge ID CATFISH ICTALURUS-PUNCTATUS; CHANNEL CATFISH; FISH; ASSOCIATION; ADHESION; DISEASE AB Flavobacterium columnare is the causative agent of columnaris disease and is re sponsible for significant economic losses in aquaculture. F. columnare is a Gram-negative bacterium, and 5 genetic types or genomovars have been described based on restriction fragment length polymorphism of the 16S rRNA gene. Previous research has suggested that genomovar II isolates are more virulent than genomovar I isolates to multiple species of fish, including rainbow trout Oncorhynchus mykiss. In addition, improved genotyping methods have shown that some isolates previously classified as genomovar I, and used in challenge experiments, were in fact genomovar III. Our objective was to confirm previous results with respect to genomovar II virulence, and to determine the susceptibility of rainbow trout to other genomovars. The virulence of 8 genomovar I, 4 genomovar II, 3 genomovar II-B, and 5 genomovar III isolates originating from various sources was determined through 3 independent challenges in rainbow trout using an immersion challenge model. Mean cumulative percent mortality ( CPM) of similar to 49% for genomovar I isolates, similar to 1% for genomovar II, similar to 5% for the II-B isolates, and similar to 7% for the III isolates was observed. The inability of genomovar II isolates to produce mortalities in rainbow trout was unanticipated based on previous studies, but may be due to a number of factors including rainbow trout source and water chemistry. The source of fish and/or the presence of sub-optimal environment may influence the susceptibility of rainbow trout to different F. columnare genomovars. C1 [Evenhuis, Jason P.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. [LaFrentz, Benjamin R.] USDA ARS, Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. RP Evenhuis, JP (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM jason.evenhuis@ars.usda.gov FU USDA-ARS CRIS Project [8082-32000-006] FX The authors thank Ryan Lipscomb, Clayton Birkett, and Travis Moreland for expert technical assistance. This work was supported by USDA-ARS CRIS Project no. 8082-32000-006. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA). USDA is an equal opportunity provider and employer. NR 23 TC 0 Z9 0 U1 5 U2 5 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD AUG 9 PY 2016 VL 120 IS 3 BP 217 EP 224 DI 10.3354/dao03027 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA DV9JL UT WOS:000383256600005 PM 27503917 ER PT J AU dos Santos, EA de Almeida, AAF Ahnert, D Branco, MCD Valle, RR Baligar, VC AF dos Santos, Emerson Alves Furtado de Almeida, Alex-Alan Ahnert, Dario da Silva Branco, Marcia Christina Valle, Raul Rene Baligar, Virupax C. TI Diallel Analysis and Growth Parameters as Selection Tools for Drought Tolerance in Young Theobroma cacao Plants SO PLOS ONE LA English DT Article ID MULTIVARIATE GENETIC-DIVERGENCE; SOUTHERN BAHIA CONDITIONS; WATER-USE EFFICIENCY; EUCALYPTUS-GLOBULUS; YIELD COMPONENTS; RESPONSES; DEFICIT; STRESS; IRRIGATION; ALLOCATION AB This study aimed to estimate the combining ability, of T. cacao genotypes preselected for drought tolerance through diallel crosses. The experiment was conducted under greenhouse conditions at the Cacao Research Center (CEPEC), Ilheus, Bahia, Brazil, in a completely randomized block design, in an experimental arrangement 21 x 2 [21 complete diallel crosses and two water regimes (control and stressed)]. In the control, soil moisture was kept close to field capacity, with predawn leaf water potential (psi(WL)) ranging from -0.1 to -0.5 MPa. In the drought regime, the soil moisture was reduced gradually by decreasing the amount of water application until psi(WL) reached -2.0 to -2.5 MPa. Significant differences (p < 0.05) were observed for most morphological attributes analyzed regarding progenies, water regime and their interactions. The results of the joint diallel analysis revealed significant effects between general combining ability (GCA) x water regimes and between specific combining ability (SCA) x water regimes. The SCA 6 genetic material showed high general combining ability for growth variables regardless of the water regime. In general, the water deficit influenced the production of biomass in most of the evaluated T. cacao crosses, except for SCA-6 x IMC-67, Catongo x SCA, MOC-01 x Catongo, Catongo x IMC-67 and RB-40 x Catongo. Multivariate analysis showed that stem diameter (CD), total leaf area (TLA), leaf dry biomass (LDB), stem dry biomass (SDB), root dry biomass (RDB), total dry biomass (TDB), root length (RL), root volume (RV), root diameter (RD) < 1 mm and 1 <(RD) < 2 mm were the most important growth parameters in the separation of T. cacao genotypes in to tolerant and intolerant to soil water deficit. C1 [dos Santos, Emerson Alves; Furtado de Almeida, Alex-Alan; Ahnert, Dario; da Silva Branco, Marcia Christina] Univ Estadual Santa Cruz, Dept Ciencias Biol, Campus Soane Nazare de Andrade,Rod Jorge Amado, BR-45662900 Ilheus, BA, Brazil. [Valle, Raul Rene] Comissao Execut Plano Lavoura Cacaueira CEPEC CEP, Ctr Pesquisas Cacau, Rod Jorge Amado,Km 22, BR-45650000 Ilheus, BA, Brazil. [Baligar, Virupax C.] USDA ARS, Beltsville, MD 20705 USA. RP de Almeida, AAF (reprint author), Univ Estadual Santa Cruz, Dept Ciencias Biol, Campus Soane Nazare de Andrade,Rod Jorge Amado, BR-45662900 Ilheus, BA, Brazil. EM alexalan@uesc.br NR 68 TC 0 Z9 0 U1 7 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 9 PY 2016 VL 11 IS 8 AR e0160647 DI 10.1371/journal.pone.0160647 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3IK UT WOS:000381374200050 PM 27504627 ER PT J AU Jeong, YH Lu, HL Park, CH Li, MY Luo, HJ Kim, JJ Liu, SY Ko, KH Huang, SJ Hwang, IS Kang, MN Gong, DS Park, KB Choi, EJ Park, JH Jeong, YW Moon, CJ Hyun, SH Kim, NH Jeung, EB Yang, HM Hwang, WS Gao, F AF Jeong, Young-Hee Lu, Hanlin Park, Chi-Hun Li, Meiyan Luo, Huijuan Kim, Joung Joo Liu, Siyang Ko, Kyeong Hee Huang, Shujia Hwang, In Sung Kang, Mi Na Gong, Desheng Park, Kang Bae Choi, Eun Ji Park, Jung Hyun Jeong, Yeon Woo Moon, Changjong Hyun, Sang-Hwan Kim, Nam Hyung Jeung, Eui-Bae Yang, Huanming Hwang, Woo Suk Gao, Fei TI Stochastic anomaly of methylome but persistent SRY hypermethylation in disorder of sex development in canine somatic cell nuclear transfer SO SCIENTIFIC REPORTS LA English DT Article ID DNA-SEQUENCING DATA; EXPRESSION; GENOME; TESTIS; METHYLATION; REVERSAL; GENES; MOUSE; SOX9; DISCOVERY AB Somatic cell nuclear transfer (SCNT) provides an excellent model for studying epigenomic reprogramming during mammalian development. We mapped the whole genome and whole methylome for potential anomalies of mutations or epimutations in SCNT-generated dogs with XY chromosomal sex but complete gonadal dysgenesis, which is classified as 78, XY disorder of sex development (DSD). Whole genome sequencing revealed no potential genomic variations that could explain the pathogenesis of DSD. However, extensive but stochastic anomalies of genome-wide DNA methylation were discovered in these SCNT DSD dogs. Persistent abnormal hypermethylation of the SRY gene was observed together with its down-regulated mRNA and protein expression. Failure of SRY expression due to hypermethylation was further correlated with silencing of a serial of testis determining genes, including SOX9, SF1, SOX8, AMH and DMRT1 in an early embryonic development stage at E34 in the XYDSD gonad, and high activation of the female specific genes, including FOXL2, RSPO1, CYP19A1, WNT4, ERa and ER beta, after one postnatal year in the ovotestis. Our results demonstrate that incomplete demethylation on the SRY gene is the driving cause of XYDSD in these XY DSD dogs, indicating a central role of epigenetic regulation in sex determination. C1 [Jeong, Young-Hee; Park, Chi-Hun; Kim, Joung Joo; Ko, Kyeong Hee; Hwang, In Sung; Kang, Mi Na; Park, Kang Bae; Choi, Eun Ji; Park, Jung Hyun; Jeong, Yeon Woo; Hyun, Sang-Hwan; Hwang, Woo Suk] Sooam Biotech Res Fdn, Seoul 152904, South Korea. [Lu, Hanlin; Li, Meiyan; Luo, Huijuan; Liu, Siyang; Huang, Shujia; Gong, Desheng; Yang, Huanming; Gao, Fei] BGI Shenzhen, Shenzhen, Peoples R China. [Lu, Hanlin; Gong, Desheng; Gao, Fei] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen, Peoples R China. [Park, Chi-Hun] USDA, Anim Biosci & Biotechnol Lab, Beltsville, MD 20705 USA. [Park, Chi-Hun] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Moon, Changjong] Chonnam Natl Univ, Coll Vet Med, Kwangju 500757, South Korea. [Hyun, Sang-Hwan; Jeung, Eui-Bae] Chungbuk Natl Univ, Coll Vet Med, Cheongju 361763, Chungbuk, South Korea. [Kim, Nam Hyung] Chungbuk Natl Univ, Coll Anim Sci, Cheongju 361763, Chungbuk, South Korea. RP Hwang, WS (reprint author), Sooam Biotech Res Fdn, Seoul 152904, South Korea.; Gao, F (reprint author), BGI Shenzhen, Shenzhen, Peoples R China.; Gao, F (reprint author), Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen, Peoples R China. EM hwangws@sooam.org; flys828@gmail.com OI Park, Chi-Hun/0000-0002-7618-9028 FU BGI-Shenzhen; Agricultural Science and Technology Innovation Program (ASTIP) of China; National Natural Science Foundation of China [81301777]; BioGreen21 Program of Rural Developmental Administration [PJ01107702]; Korean National Police Agency, Republic of Korea [12148249800] FX This work was supported by BGI-Shenzhen, the Agricultural Science and Technology Innovation Program (ASTIP) of China, the National Natural Science Foundation of China (81301777), BioGreen21 Program (#PJ01107702) of Rural Developmental Administration and Korean National Police Agency (#12148249800), Republic of Korea. NR 41 TC 0 Z9 0 U1 5 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD AUG 9 PY 2016 VL 6 AR 31088 DI 10.1038/srep31088 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS8IR UT WOS:000381027200001 PM 27501986 ER PT J AU Kumar, P He, YJ Singh, R Davis, RF Guo, H Paterson, AH Peterson, DG Shen, XL Nichols, RL Chee, PW AF Kumar, Pawan He, Yajun Singh, Rippy Davis, Richard F. Guo, Hui Paterson, Andrew H. Peterson, Daniel G. Shen, Xinlian Nichols, Robert L. Chee, Peng W. TI Fine mapping and identification of candidate genes for a QTL affecting Meloidogyne incognita reproduction in Upland cotton SO BMC GENOMICS LA English DT Article DE Cotton; Root Knot Nematodes; RKN; QTLs; SNP; Candidate genes ID ROOT-KNOT NEMATODE; GOSSYPIUM-HIRSUTUM L.; TRANSGRESSIVE SEGREGATION; RESISTANCE GENES; GERMPLASM LINE; FAMILY; REGISTRATION; PENETRATION; POPULATION; BARBADENSE AB Background: The southern root-knot nematode (Meloidogyne incognita; RKN) is one of the most important economic pests of Upland cotton (Gossypium hirsutum L.). Host plant resistance, the ability of a plant to suppress nematode reproduction, is the most economical, practical, and environmentally sound method to provide protection against this subterranean pest. The resistant line Auburn 623RNR and a number of elite breeding lines derived from it remain the most important source of root-knot nematode (RKN) resistance. Prior genetic analysis has identified two epistatically interacting RKN resistance QTLs, qMi-C11 and qMi-C14, affecting gall formation and RKN reproduction, respectively. Results: We developed a genetic population segregating only for the qMi-C14 locus and evaluated the genetic effects of this QTL on RKN resistance in the absence of the qMi-C11 locus. The qMi-C14 locus had a LOD score of 12 and accounted for 24.5 % of total phenotypic variation for egg production. In addition to not being significantly associated with gall formation, this locus had a lower main effect on RKN reproduction than found in our previous study, which lends further support to evidence of epistasis with qMi-C11 in imparting RKN resistance in the Auburn 623RNR source. The locus qMi-C14 was fine-mapped with the addition of 16 newly developed markers. By using the reference genome sequence of G. raimondii, we identified 20 candidate genes encoding disease resistance protein homologs in the newly defined 2.3 Mb region flanked by two SSR markers. Resequencing of an RKN resistant and susceptible G. hirsutum germplasm revealed non-synonymous mutations in only four of the coding regions of candidate genes, and these four genes are consequently of high interest. Conclusions: Our mapping results validated the effects of the qMi-C14 resistance locus, delimiting the QTL to a smaller region, and identified tightly linked SSR markers to improve the efficiency of marker-assisted selection. The candidate genes identified warrant functional studies that will help in identifying and characterizing the actual qMi-C14 defense gene(s) against root-knot nematodes. C1 [Kumar, Pawan; He, Yajun; Singh, Rippy; Shen, Xinlian; Chee, Peng W.] Univ Georgia, Cotton Mol Breeding Lab, Tifton, GA 31793 USA. [Davis, Richard F.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Guo, Hui; Paterson, Andrew H.] Univ Georgia, Plant Genome Mapping Lab, Athens, GA 30602 USA. [Peterson, Daniel G.] Mississippi State Univ, Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39762 USA. [Nichols, Robert L.] Cotton Inc, 6399 Weston Pkwy, Cary, NC 27513 USA. [Shen, Xinlian] Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing 210014, Jiangsu, Peoples R China. [He, Yajun] Southwest Univ, Coll Agron & Biotechnol, Chongqing 400716, Peoples R China. RP Chee, PW (reprint author), Univ Georgia, Cotton Mol Breeding Lab, Tifton, GA 31793 USA. EM pwchee@uga.edu FU Georgia Cotton Commission; Cotton Incorporated. FX We appreciate financial support from the Georgia Cotton Commission and Cotton Incorporated. NR 48 TC 0 Z9 0 U1 9 U2 9 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 8 PY 2016 VL 17 AR 567 DI 10.1186/s12864-016-2954-1 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DT1FC UT WOS:000381226100007 PM 27503539 ER PT J AU Zhu, HY Song, PY Koo, DH Guo, LQ Li, YM Sun, SR Weng, YQ Yang, LM AF Zhu, Huayu Song, Pengyao Koo, Dal-Hoe Guo, Luqin Li, Yanman Sun, Shouru Weng, Yiqun Yang, Luming TI Genome wide characterization of simple sequence repeats in watermelon genome and their application in comparative mapping and genetic diversity analysis SO BMC GENOMICS LA English DT Article DE SSR; Watermelon; Comparative genomics; Synteny; Cucurbits; Genetic diversity ID CUCUMIS-SATIVUS L.; F-SP NIVEUM; CITRULLUS-LANATUS; MICROSATELLITE MARKERS; SSR-MARKERS; MOLECULAR PHYLOGENETICS; POPULATION-STRUCTURE; DRAFT GENOME; LINKAGE MAP; MELO L. AB Background: Microsatellite markers are one of the most informative and versatile DNA-based markers used in plant genetic research, but their development has traditionally been difficult and costly. The whole genome sequencing with next-generation sequencing (NGS) technologies provides large amounts of sequence data to develop numerous microsatellite markers at whole genome scale. SSR markers have great advantage in cross-species comparisons and allow investigation of karyotype and genome evolution through highly efficient computation approaches such as in silico PCR. Here we described genome wide development and characterization of SSR markers in the watermelon (Citrullus lanatus) genome, which were then use in comparative analysis with two other important crop species in the Cucurbitaceae family: cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). We further applied these markers in evaluating the genetic diversity and population structure in watermelon germplasm collections. Results: A total of 39,523 microsatellite loci were identified from the watermelon draft genome with an overall density of 111 SSRs/Mbp, and 32,869 SSR primers were designed with suitable flanking sequences. The dinucleotide SSRs were the most common type representing 34.09 % of the total SSR loci and the AT-rich motifs were the most abundant in all nucleotide repeat types. In silico PCR analysis identified 832 and 925 SSR markers with each having a single amplicon in the cucumber and melon draft genome, respectively. Comparative analysis with these crossspecies SSR markers revealed complicated mosaic patterns of syntenic blocks among the genomes of three species. In addition, genetic diversity analysis of 134 watermelon accessions with 32 highly informative SSR loci placed these lines into two groups with all accessions of C.lanatus var. citorides and three accessions of C. colocynthis clustered in one group and all accessions of C. lanatus var. lanatus and the remaining accessions of C. colocynthis clustered in another group. Furthermore, structure analysis was consistent with the dendrogram indicating the 134 watermelon accessions were classified into two populations. Conclusion: The large number of genome wide SSR markers developed herein from the watermelon genome provides a valuable resource for genetic map construction, QTL exploration, map-based gene cloning and marker-assisted selection in watermelon which has a very narrow genetic base and extremely low polymorphism among cultivated lines. Furthermore, the cross-species transferable SSR markers identified herein should also have practical uses in many applications in species of Cucurbitaceae family whose whole genome sequences are not yet available. C1 [Zhu, Huayu; Song, Pengyao; Guo, Luqin; Li, Yanman; Sun, Shouru; Yang, Luming] Henan Agr Univ, Coll Hort, 63 Nongye Rd, Zhengzhou 450002, Peoples R China. [Koo, Dal-Hoe; Weng, Yiqun] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. [Weng, Yiqun] USDA ARS, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. RP Yang, LM (reprint author), Henan Agr Univ, Coll Hort, 63 Nongye Rd, Zhengzhou 450002, Peoples R China.; Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM weng4@wisc.edu; ylmhort@163.com FU Scientific Innovation Fund of Henan Agricultural University [KJCX2015A11]; Initial Funding for the introduction of talents of Henan Agricultural University [30600860] FX This research was supported by Scientific Innovation Fund of Henan Agricultural University (KJCX2015A11) and The Initial Funding for the introduction of talents of Henan Agricultural University (30600860). NR 63 TC 1 Z9 1 U1 34 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 5 PY 2016 VL 17 AR 557 DI 10.1186/s12864-016-2870-4 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DT1EY UT WOS:000381225700008 PM 27495254 ER PT J AU Douds, DD Lee, J McKeever, L Ziegler-Ulsh, C Ganser, S AF Douds, David D., Jr. Lee, Joe McKeever, Lindsay Ziegler-Ulsh, Christine Ganser, Steven TI Utilization of inoculum of AM fungi produced on-farm increases the yield of Solanum lycopersicum: A summary of 7 years of field trials on a conventional vegetable farm with high soil phosphorus SO SCIENTIA HORTICULTURAE LA English DT Article DE Sustainable agriculture; Tomato; Biofertilizer; Mycorrhizal yield response ID ARBUSCULAR MYCORRHIZAL FUNGI; PLANT-GROWTH; ENDOGONE MYCORRHIZA; NUTRIENT-UPTAKE; CROPPING SYSTEMS; TOMATO; INOCULATION; INFECTION; RESPONSES; COLONIZATION AB Adding arbuscular mycorrhizal [AM] fungus inoculum to potting media enables vegetable farmers to better take advantage of the AM symbiosis. On-farm production of AM fungus inoculum is a viable alternative to commercially-available inocula. We conducted a seven year experiment at a conventional vegetable farm in southeastern Pennsylvania with high soil available P typical of the Mid-Atlantic region of the USA (210 mg kg-1 soil). Seedlings of three to seven cultivars of Solanum lycopersicum L were inoculated in the greenhouse phase of production with a mixed species inoculum of AM fungi produced on-farm. Performance of the inoculation treatment was evaluated based on growth response in the greenhouse and fruit production in the field compared to uninoculated controls. Colonization levels were typically only 1-10% of root length at the time of outplanting. The mean mycorrhizal growth dependency based upon shoot growth at the time of outplanting was significant at 12.5 +/- 3.55%(alpha=0.05). In addition, the mean mycorrhizal yield response over the seven year experiment was significant at 6.02 +/- 1.92% (alpha=0.05). Routine use of AM fungus inoculum produced on-farm provided a modest but significant increase in yield of tomato fruit with minimal change in farm management even in a high P soil. Published by Elsevier B.V. C1 [Douds, David D., Jr.; Lee, Joe; McKeever, Lindsay] USDA ARS ERRC, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. [Ziegler-Ulsh, Christine] Rodale Inst Expt Farm, 611 Siegfriedale Rd, Kutztown, PA 19530 USA. [Ganser, Steven] Eagle Point Farm, 477 Hottenstein Rd, Kutztown, PA 19530 USA. [Ziegler-Ulsh, Christine] USDA NRCS, Berks Cty Agr Ctr, 1238 Cty Welf Rd,Suite 220, Leesport, PA 19533 USA. RP Douds, DD (reprint author), USDA ARS ERRC, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.douds@ars.usda.gov FU USDA-CSRSEES Sustainable Agriculture Research and Education program [LNE03-179] FX This work was supported in part with support from grant no. LNE03-179 from the USDA-CSRSEES Sustainable Agriculture Research and Education program. We would like to thank S. Campbell, M.E. Lohman, N. Pinzon, and the many Rodale Institute interns who helped with harvesting and inoculum production over the years of this experiment. NR 76 TC 1 Z9 1 U1 30 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 EI 1879-1018 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD AUG 5 PY 2016 VL 207 BP 89 EP 96 DI 10.1016/j.scienta.2016.05.013 PG 8 WC Horticulture SC Agriculture GA DR4QR UT WOS:000379887800013 ER PT J AU Abdelfattah, A Wisniewski, M Nicosia, MGL Cacciola, SO Schena, L AF Abdelfattah, Ahmed Wisniewski, Michael Nicosia, Maria Giulia Li Destri Cacciola, Santa Olga Schena, Leonardo TI Metagenomic Analysis of Fungal Diversity on Strawberry Plants and the Effect of Management Practices on the Fungal Community Structure of Aerial Organs SO PLOS ONE LA English DT Article ID AUREOBASIDIUM-PULLULANS; MICROBIAL COMMUNITIES; ERYSIPHE-AQUILEGIAE; POSTHARVEST ROTS; SWEET CHERRIES; TABLE GRAPES; BOTRYTIS; FRUIT; QUALITY; SEARCH AB An amplicon metagenomic approach based on the ITS2 region of fungal rDNA was used to identify the composition of fungal communities associated with different strawberry organs (leaves, flowers, immature and mature fruits), grown on a farm using management practices that entailed the routine use of various chemical pesticides. ITS2 sequences clustered into 316 OTUs and Ascomycota was the dominant phyla (95.6%) followed by Basidiomycota (3.9%). Strawberry plants supported a high diversity of microbial organisms, but two genera, Botrytis and Cladosporium, were the most abundant, representing 70-99% of the relative abundance (RA) of all detected sequences. According to alpha and beta diversity analyses, strawberry organs displayed significantly different fungal communities with leaves having the most diverse fungal community, followed by flowers, and fruit. The interruption of chemical treatments for one month resulted in a significant modification in the structure of the fungal community of leaves and flowers while immature and mature fruit were not significantly affected. Several plant pathogens of other plant species, that would not be intuitively expected to be present on strawberry plants such as Erysiphe, were detected, while some common strawberry pathogens, such as Rhizoctonia, were less evident or absent. C1 [Abdelfattah, Ahmed; Nicosia, Maria Giulia Li Destri; Schena, Leonardo] Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89122 Reggio Di Calabria, Italy. [Wisniewski, Michael] ARS, USDA, AFRS, 2217 Wiltshire Rd, Kearneysville, WV USA. [Cacciola, Santa Olga] Univ Catania, Dipartimento Agr Alimentazione & Ambiente, Via S Sofia 100, I-95123 Catania, Italy. RP Schena, L (reprint author), Univ Mediterranea Reggio Calabria, Dipartimento Agr, I-89122 Reggio Di Calabria, Italy. EM lschena@unirc.it OI Schena, Leonardo/0000-0002-9737-2593; Abdelfattah, Ahmed/0000-0001-6090-7200 FU Italian Ministry of Education, University and Research (MIUR) [PON03PE_00090_03] FX This work was funded by The Italian Ministry of Education, University and Research (MIUR) with grant "Modelli sostenibili e nuove tecnologie per la valorizzazione delle filiere vegetali mediterranee"-PON Ricerca e competitivita 2007-2013 (PON03PE_00090_03). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; This work was funded by The Italian Ministry of Education, University and Research (MIUR) with grant "Modelli sostenibili e nuove tecnologie per la valorizzazione delle filiere vegetali mediterranee"-PON Ricerca e competitivita 2007-2013 (PON03PE_00090_03). NR 49 TC 1 Z9 1 U1 10 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 4 PY 2016 VL 11 IS 8 AR e0160470 DI 10.1371/journal.pone.0160470 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT3GL UT WOS:000381368900060 PM 27490110 ER PT J AU Dhanapal, AP Ray, JD Singh, SK Hoyos-Villegas, V Smith, JR Purcell, LC Fritschi, FB AF Dhanapal, Arun Prabhu Ray, Jeffery D. Singh, Shardendu K. Hoyos-Villegas, Valerio Smith, James R. Purcell, Larry C. Fritschi, Felix B. TI Genome-wide association mapping of soybean chlorophyll traits based on canopy spectral reflectance and leaf extracts SO BMC PLANT BIOLOGY LA English DT Article DE Abiotic stress tolerance; Chlorophyll a; Chlorophyll b; Chlorophyll a/b ratio; Total chlorophyll; Genome-wide association mapping; Single nucleotide polymorphisms; High-throughput phenotyping ID ORYZA-SATIVA L.; PHYSIOLOGICAL TRAITS; WAVELET DECOMPOSITION; OPTICAL-PROPERTIES; NITROGEN-CONTENT; LEAVES; PHOTOSYNTHESIS; RICE; PLANTS; YIELD AB Background: Chlorophyll is a major component of chloroplasts and a better understanding of the genetic basis of chlorophyll in soybean [Glycine max (L.) Merr.] might contribute to improving photosynthetic capacity and yield in regions with adverse environmental conditions. A collection of 332 diverse soybean genotypes were grown in 2 years (2009 and 2010) and chlorophyll a (eChl_A), chlorophyll b (eChl_B), and total chlorophyll (eChl_T) content as well as chlorophyll a/b ratio (eChl_R) in leaf tissues were determined by extraction and spectrometric determination. Total chlorophyll was also derived from canopy spectral reflectance measurements using a model of wavelet transformed spectra (tChl_T) as well as with a spectral reflectance index (iChl_T). Results: A genome-wide associating mapping approach was employed using 31,253 single nucleotide polymorphisms (SNPs) to identify loci associated with the extract based eChl_A, eChl_B, eChl_R and eChl_T measurements and the two canopy spectral reflectance-based methods (tChl_T and iChl_T). A total of 23 (14 loci), 15 (7 loci) and 14 SNPs (10 loci) showed significant association with eChl_A, eChl_B and eChl_R respectively. A total of 52 unique SNPs were significantly associated with total chlorophyll content based on at least one of the three approaches (eChl_T, tChl_T and iChl_T) and likely tagged 27 putative loci for total chlorophyll content, four of which were indicated by all three approaches. Conclusions: Results presented here show that markers for chlorophyll traits can be identified in soybean using both extract-based and canopy spectral reflectance-based phenotypes, and confirm that high-throughput phenotyping-amenable canopy spectral reflectance measurements can be used for association mapping. C1 [Dhanapal, Arun Prabhu; Fritschi, Felix B.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Ray, Jeffery D.; Smith, James R.] ARS, Crop Genet Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. [Singh, Shardendu K.] ARS, Crop Syst & Global Change Lab, USDA, Beltsville, MD 20705 USA. [Hoyos-Villegas, Valerio] Lincoln Sci Ctr, Forage Improvement Grp, Christchurch 8140, New Zealand. [Purcell, Larry C.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. RP Fritschi, FB (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM fritschif@missouri.edu OI Dhanapal, Arun Prabhu/0000-0002-9686-7260 FU United States Department of Agriculture-Agriculture Research Service (USDA-ARS) [6402-21220-010-00D]; United Soybean Board [9274, 1274] FX This work was supported by United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project number 6402-21220-010-00D and United Soybean Board project numbers 9274 and 1274. NR 82 TC 0 Z9 0 U1 13 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 4 PY 2016 VL 16 AR 174 DI 10.1186/s12870-016-0861-x PG 15 WC Plant Sciences SC Plant Sciences GA DS4WP UT WOS:000380782600001 PM 27488358 ER PT J AU Sebastian, J Yee, MC Viana, WG Rellan-Alvarez, R Feldman, M Priest, HD Trontin, C Lee, T Jiang, H Baxter, I Mockler, TC Hochholdinger, F Brutnell, TP Dinneny, JR AF Sebastian, Jose Yee, Muh-Ching Viana, Willian Goudinho Rellan-Alvarez, Ruben Feldman, Max Priest, Henry D. Trontin, Charlotte Lee, Tak Jiang, Hui Baxter, Ivan Mockler, Todd C. Hochholdinger, Frank Brutnell, Thomas P. Dinneny, Jose R. TI Grasses suppress shoot-borne roots to conserve water during drought SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE root development; drought; Poaceae; Setaria; Zea mays ID MILLET SETARIA-ITALICA; MODEL GENETIC SYSTEMS; NITROGEN ACQUISITION; NODAL ROOTS; MAIZE; VIRIDIS; ZEA; L.; INITIATION; CEREALS AB Many important crops are members of the Poaceae family, which develop root systems characterized by a high degree of root initiation from the belowground basal nodes of the shoot, termed the crown. Although this postembryonic shoot-borne root system represents the major conduit for water uptake, little is known about the effect of water availability on its development. Here we demonstrate that in the model C4 grass Setaria viridis, the crown locally senses water availability and suppresses postemergence crown root growth under a water deficit. This response was observed in field and growth room environments and in all grass species tested. Luminescence-based imaging of root systems grown in soil-like media revealed a shift in root growth from crown-derived to primary root-derived branches, suggesting that primary root-dominated architecture can be induced in S. viridis under certain stress conditions. Crown roots of Zea mays and Setaria italica, domesticated relatives of teosinte and S. viridis, respectively, show reduced sensitivity to water deficit, suggesting that this response might have been influenced by human selection. Enhanced water status of maize mutants lacking crown roots suggests that under a water deficit, stronger suppression of crown roots actually may benefit crop productivity. C1 [Sebastian, Jose; Yee, Muh-Ching; Viana, Willian Goudinho; Rellan-Alvarez, Ruben; Trontin, Charlotte; Dinneny, Jose R.] Carnegie Inst Sci, Dept Plant Biol, 290 Panama St, Stanford, CA 94305 USA. [Viana, Willian Goudinho] Minist Educ Brazil, Coordinat Improvement Higher Educ Personnel CAPES, BR-70040020 Brasilia, DF, Brazil. [Rellan-Alvarez, Ruben] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Lab Nacl Genom Biodiversidad, Unidad Genom Avanzada, Irapuato 36821, Mexico. [Feldman, Max; Priest, Henry D.; Jiang, Hui; Baxter, Ivan; Mockler, Todd C.; Brutnell, Thomas P.] Donald Danforth Plant Sci Ctr, St Louis, MO 63162 USA. [Lee, Tak] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biotechnol, Seoul 03722, South Korea. [Baxter, Ivan] USDA, Plant Physiol & Genet Res, Agr Res Unit, St Louis, MO 63132 USA. [Hochholdinger, Frank] Univ Bonn, Inst Crop Sci & Resource Conservat, Crop Funct Genom, D-53113 Bonn, Germany. RP Dinneny, JR (reprint author), Carnegie Inst Sci, Dept Plant Biol, 290 Panama St, Stanford, CA 94305 USA. EM jdinneny@carnegiescience.edu RI Baxter, Ivan/A-1052-2009; Mockler, Todd/L-2609-2013; Brutnell, Thomas/M-2840-2013; OI Baxter, Ivan/0000-0001-6680-1722; Mockler, Todd/0000-0002-0462-5775; Brutnell, Thomas/0000-0002-3581-8211; Rellan-Alvarez, Ruben/0000-0001-6843-3716 FU US Department of Energy's Biological and Environmental Research program [DE-SC0008769]; National Science Foundation's Plant Genome Research Program [IOS-PGRP 420-40-45A] FX We thank Neil Robbins II and Josep Vilarrasa-Blasi for comments on the manuscript; Therese LaRue for help with figure preparation; Neil Robbins II for bulking of rtcs/+ seeds; the US Department of Agriculture (North Central Regional Plant Introduction Station, Iowa State University), Torbert Rocheford (Purdue University), and John Vogel for seeds; and Todd Tobeck (Department of Global Ecology, Carnegie Institution for Science) for help with field soil moisture measurements. Funding was provided by the US Department of Energy's Biological and Environmental Research program (Grant DE-SC0008769, to T.P.B., T.C.M., I.B., and J.R.D.) and the National Science Foundation's Plant Genome Research Program (Grant IOS-PGRP 420-40-45A, to J.R.D.). NR 47 TC 3 Z9 3 U1 17 U2 19 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD AUG 2 PY 2016 VL 113 IS 31 BP 8861 EP 8866 DI 10.1073/pnas.1604021113 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS2HH UT WOS:000380586600078 PM 27422554 ER PT J AU Tai, SHS Holz, C Engstrom, MD Cheng, HH Maes, RK AF Tai, S. -H. Sheldon Holz, Carine Engstrom, Michael D. Cheng, Hans H. Maes, Roger K. TI In vitro characterization of felid herpesvirus 1 (FHV-1) mutants generated by recombineering in a recombinant BAC vector SO VIRUS RESEARCH LA English DT Article DE Felid herpesvirus 1; Bacterial artificial chromosome; Vaccine; Glycoprotein; Serine/threonine protein kinase; Thymidine kinase ID TO-CELL SPREAD; PSEUDORABIES VIRUS; GLYCOPROTEIN-C; GE; TYPE-1; GI; DELETION; VACCINE; GENOME; BHV-1 AB Felid herpesvirus 1 (FHV-1) mutants were constructed using two-step Red-mediated recombination techniques based on a virulent full-length FHV-1 BAC clone. The individual mutant viruses generated were deficient in glycoprotein C (gC), glycoprotein E (gE), US3 serine/threonine protein kinase (PK), or both gE and thymidine kinase (TK). The gC- mutant virus produced plaques that were similar in size to those resulting from infection with the C-27 parent strain. In contrast, the gE(-), PK-, and gE(-)PK(-) deletion mutants produced plaques that were significantly smaller. Multistep in vitro growth kinetics of the gE(-), PK-, and gE(-)PK(-) viruses were slightly delayed compared to those of the C-27 parent strain. Peak progeny titers of these three mutants were approximately 10-fold lower than those generated with the C-27 strain. There was no delay in the growth kinetics of the gC- mutant, but the progeny virus titer obtained with this mutant was at least 3 logs lower compared to the parental strain titer. Based upon their in vitro characteristics, these mutants will be useful for the development of novel immunization strategies against this important feline pathogen. (C) 2016 Elsevier B.V. All rights reserved. C1 [Tai, S. -H. Sheldon; Maes, Roger K.] Michigan State Univ, Coll Vet Med, Grad Program Comparat Med & Integrat Biol, E Lansing, MI 48824 USA. [Tai, S. -H. Sheldon; Holz, Carine; Maes, Roger K.] Michigan State Univ, Coll Vet Med, Diagnost Ctr Populat & Anim Hlth, 4125 Beaumont Rd, Lansing, MI 48910 USA. [Engstrom, Michael D.; Maes, Roger K.] Michigan State Univ, Dept Microbiol & Mol Genet, Coll Vet Med, E Lansing, MI 48824 USA. [Cheng, Hans H.] ARS, USDA, Avian Dis & Oncol Lab, 4279 E Mt Hope Rd, E Lansing, MI 48823 USA. [Maes, Roger K.] Michigan State Univ, Coll Vet Med, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA. RP Maes, RK (reprint author), Michigan State Univ, Coll Vet Med, Dept Pathobiol & Diagnost Invest, E Lansing, MI 48824 USA. EM Maes@dcpah.msu.edu FU Michigan State University College of Veterinary Medicine's Feline Health Endowment; MSU Center for Feline Health and Well-Being; Department of Pathobiology and Diagnostic Investigation FX Funding for this research was provided by Michigan State University College of Veterinary Medicine's Feline Health Endowment, the MSU Center for Feline Health and Well-Being and the Department of Pathobiology and Diagnostic Investigation. The authors thank Biological Resources Branch at National Cancer Institute for providing E. coli SW105 cells and Dr. Nikolaus Osterrieder for generously providing plasmids essential for two-step Red-mediated recombination. The authors also thank Drs. Taejoong Kim and Masahiro Niikura for generously providing essential reagents and advice, and Laurie Molitor for excellent technical assistance. NR 32 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD AUG 2 PY 2016 VL 221 BP 15 EP 22 DI 10.1016/j.virusres.2016.05.001 PG 8 WC Virology SC Virology GA DQ3ML UT WOS:000379106300003 PM 27157860 ER PT J AU Smiley, RW Machado, S Rhinhart, KEL Reardon, CL Wuest, SB AF Smiley, Richard W. Machado, Stephen Rhinhart, Karl E. L. Reardon, Catherine L. Wuest, Stewart B. TI Rapid Quantification of Soilborne Pathogen Communities in Wheat-Based Long-Term Field Experiments SO PLANT DISEASE LA English DT Article ID SEMIARID PACIFIC-NORTHWEST; POLYMERASE-CHAIN-REACTION; RHIZOCTONIA-SOLANI AG-8; SOIL DNA CONCENTRATIONS; WINTER-WHEAT; PRATYLENCHUS-NEGLECTUS; ROOT-ROT; TAKE-ALL; EASTERN WASHINGTON; RESIDUE MANAGEMENT AB Rainfed experiments operated continuously for up to 84 years in semiarid eastern Oregon are among the oldest agronomic trials in North America. Disease incidence and severity had been quantified visually but quantification of inoculum density had not been attempted. Natural inoculum of 17 fungal and nematode pathogens were quantified for each of 2 years on eight trials using DNA extracts from soil. Crop type, tillage, rotation, soil fertility, year, and their interactions had large effects on the pathogens. Fusarium culmorum and Pratylenchus thornei were more dominant than F. pseudograminearum and P. neglectus where spring crops were grown, and the opposite species dominances occurred where winter wheat was the only crop. Bipolaris sorokiniana and Phoma pinodella were restricted to the presence of spring cereals and pulse crops, respectively. Helgardia spp. occurred in winter wheat-fallow rotations but not in annual winter wheat. Gaeumannomyces graminis var. tritici was more prevalent in cultivated than noncultivated soils and the opposite generally occurred for Rhizoctonia solani AG-8. Densities of Pythium spp. Glade F were high but were also influenced by treatments. Significant treatment effects and interactions were more prevalent in two long-standing (>50-year) annually cropped experiments (29%) than two long-standing 2-year wheat fallow rotations (14%). Associations among pathogens occurred mostly in an 84-year-old annual cereals experiment. This survey provided guidance for research on dynamics of root-infecting pathogens of rainfed field crops and identified two pathogens (Drechslera tritici-repentis and P. pinodella) not previously identified at the location. C1 [Smiley, Richard W.; Machado, Stephen; Rhinhart, Karl E. L.] Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. [Reardon, Catherine L.; Wuest, Stewart B.] USDA ARS, Columbia Plateau Conservat Res Ctr, Pendleton, OR 97801 USA. RP Smiley, RW (reprint author), Oregon State Univ, Columbia Basin Agr Res Ctr, Pendleton, OR 97801 USA. EM richard.smiley@oregonstate.edu FU Oregon State University Agricultural Research Foundation; Oregon Agricultural Experiment Station; United States Department of Agriculture-Agricultural Research Service; Root Disease Testing Service of the South Australian Research and Development Institute FX Funding was provided by the Oregon State University Agricultural Research Foundation, Oregon Agricultural Experiment Station, United States Department of Agriculture-Agricultural Research Service, and a discounted analysis fee by the Root Disease Testing Service of the South Australian Research and Development Institute. Sampling assistance was contributed by J. Gourlie, M. Chandler, M. Baxter, G. Yan, L. Pritchett, A. Spence, E. Tones, and M. Wuest. NR 76 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1692 EP 1708 DI 10.1094/PDIS-09-15-1020-RE PG 17 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200024 ER PT J AU Di Noia, J Monica, D Cullen, KW Perez-Escamilla, R Gray, HL Sikorskii, A AF Di Noia, Jennifer Monica, Dorothy Cullen, Karen Weber Perez-Escamilla, Rafael Gray, Heewon Lee Sikorskii, Alla TI Differences in Fruit and Vegetable Intake by Race/Ethnicity and by Hispanic Origin and Nativity Among Women in the Special Supplemental Nutrition Program for Women, Infants, and Children, 2015 SO PREVENTING CHRONIC DISEASE LA English DT Article ID UNITED-STATES; ADULTS; ACCULTURATION; CONSUMPTION; LATINOS; AVAILABILITY; SUBGROUPS; COMMUNITY; NUTRIENT; QUALITY AB Introduction The objective of this exploratory study was to determine whether fruit and vegetable consumption differed by race/ethnicity, by origin and nativity among Hispanics, and by language preference (as an indicator of acculturation) among foreign-born Hispanics. Methods We recruited 723 women enrolled in the Special Supplemental Nutrition Program for Women, Infants and Children (WIC) and orally administered a questionnaire containing demographic items, validated measures of food security status and social desirability trait, and the Behavioral Risk Factor Surveillance System fruit and vegetable module. Differences in intakes of 100% fruit juice, fruit, cooked or canned beans, and dark green, orange-colored, and other vegetables were assessed by using analysis of covariance with Bonferroni post hoc tests. Analyses were controlled for age, pregnancy status, breastfeeding status, food security status, educational attainment, and social desirability trait. Results The frequency of vegetable intake differed by race/ethnicity (cooked or canned beans were consumed more often among Hispanic than non-Hispanic black and non-Hispanic white or other participants, orange-colored vegetables were consumed more often among Hispanics than non-Hispanic black participants, and other vegetables were consumed more often among non-Hispanic white or other than among non-Hispanic black and Hispanic participants), origin (other vegetables were consumed more often among Columbian and other Hispanics than Dominican participants) and nativity (orange-colored vegetables were consumed more often among foreign-born than US-born Hispanics). Fruit and vegetable intake did not differ by language preference among foreign-born Hispanics. Conclusion Differences in fruit and vegetable consumption among WIC participants by race/ethnicity and by Hispanic origin and nativity may have implications for WIC nutrition policies and nutrition education efforts. C1 [Di Noia, Jennifer] William Paterson Univ, Dept Sociol, 300 Pompton Rd, Wayne, NJ 07470 USA. [Monica, Dorothy] St Josephs WIC Program, Paterson, NJ USA. [Cullen, Karen Weber] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Perez-Escamilla, Rafael] Yale Sch Publ Hlth, New Haven, CT USA. [Gray, Heewon Lee] Columbia Univ, Teachers Coll, New York, NY 10027 USA. [Sikorskii, Alla] Michigan State Univ, E Lansing, MI 48824 USA. RP Di Noia, J (reprint author), William Paterson Univ, Dept Sociol, 300 Pompton Rd, Wayne, NJ 07470 USA. EM dinoiaj@wpunj.edu FU US Department of Agriculture, Food and Nutrition Service [WIC NEI-12-TX] FX This article was produced under a project supported with federal funds from the US Department of Agriculture, Food and Nutrition Service, through grant WIC NEI-12-TX to Baylor College of Medicine to Jennifer Di Noia at William Paterson University. The contents of this publication do not necessarily reflect the view or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US government. NR 26 TC 0 Z9 0 U1 0 U2 0 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD AUG PY 2016 VL 13 AR 160130 DI 10.5888/pcd13.160130 PG 13 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA EJ3JE UT WOS:000393107600016 ER PT J AU Rohumaa, A Yamamoto, A Hunt, CG Frihart, CR Hughes, M Kers, J AF Rohumaa, Anti Yamamoto, Akio Hunt, Christopher G. Frihart, Charles R. Hughes, Mark Kers, Jaan TI Effect of Log Soaking and the Temperature of Peeling on the Properties of Rotary-Cut Birch (Betula pendula Roth) Veneer Bonded with Phenol-Formaldehyde Adhesive SO BIORESOURCES LA English DT Article DE Veneer; Log soaking; ABES; Bonding strength; Roughness ID QUALITY; WATER; WOOD AB Heating logs prior to peeling positively affects the surface properties of veneer as well as the wood-adhesive bond strength. However, the mechanism behind this increase in strength is not fully understood. The aim of the present study was to separate the influence of soaking temperature and peeling temperature on the physical surface properties and bonding quality. Rotary-cut birch (Betula pendula Roth) logs were soaked at 70 degrees C, and half of them were subsequently cooled to 20 degrees C prior to peeling. Surface roughness measurements, scanning electron microscopy (SEM), surface integrity testing, color measurements, and wood-adhesive bond testing were conducted with an automated bonding evaluation system. The results showed that logs soaked at 70 degrees C and peeled at 20 degrees C had roughness, color, integrity, bond strength, and wetting properties more similar to logs soaked and peeled at 70 degrees C than those soaked and peeled at 20 degrees C. In every test conducted, the effect of soaking temperature was greater than the effect of peeling temperature. High-temperature soaking not only caused softening of the material during the peeling process, but it also caused irreversible changes in the wood material, which affected the veneer surface characteristics and bond strength development. C1 [Rohumaa, Anti; Yamamoto, Akio; Hughes, Mark] Aalto Univ, Sch Chem Technol, Dept Forest Prod Technol, Vuorimiehentie 1, Espoo 02150, Finland. [Hunt, Christopher G.; Frihart, Charles R.] US Forest Serv, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Kers, Jaan] Tallinn Univ Technol, Dept Polymer Mat, Ehitajate Tee 5, EE-19086 Tallinn, Estonia. RP Rohumaa, A (reprint author), Aalto Univ, Sch Chem Technol, Dept Forest Prod Technol, Vuorimiehentie 1, Espoo 02150, Finland. EM anti.rohumaa@aalto.fi RI Hughes, Mark/G-2249-2013 FU Finnish Funding Agency for Technology and Innovation (Tekes) FX The authors would like to acknowledge the financial assistance provided by the Finnish Funding Agency for Technology and Innovation (Tekes). Additionally, warm thanks are extended to the staff at the U. S. Forest Service Forest Products Laboratory in Madison, WI, USA. NR 18 TC 1 Z9 1 U1 1 U2 1 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD AUG PY 2016 VL 11 IS 3 BP 5829 EP 5838 DI 10.15376/biores.11.3.5829-5838 PG 10 WC Materials Science, Paper & Wood SC Materials Science GA DY2KV UT WOS:000384922400015 ER PT J AU Cheng, XY He, X Xie, J Quan, P Xu, K Li, XJ Cai, ZY AF Cheng, Xiyi He, Xia Xie, Jie Quan, Peng Xu, Kang Li, Xianjun Cai, Zhiyong TI Effect of the Particle Geometry and Adhesive Mass Percentage on the Physical and Mechanical Properties of Particleboard made from Peanut Hull SO BIORESOURCES LA English DT Article DE Peanut hull particleboard; Urea-formaldehyde; Polymeric diphenylmethane diisocyanate; Physical and mechanical properties; Microstructure; Chemical composition ID SUNFLOWER STALKS; RAW-MATERIAL; WOOD CHIPS; L.; FIBER; PRETREATMENT; MANUFACTURE; RESIN; BARK; PULP AB Peanut hull residues were considered for the manufacturing of particleboards. Various concentrations of two types of adhesive-polymeric diphenylmethane diisocyanate (MDI) and urea-formaldehyde (UF)-were separately combined with four types of peanut hull particles (fine, mixed, coarse particles, and peanut hull powder) to manufacture particleboards with a certain target density. The confidence level of the effect of the selected production parameters on the physical and mechanical properties of the panels was evaluated. The results showed that increasing the adhesive mass percentage significantly improved the dimensional stability of the boards. A better mechanical performance was achieved for the MDI-bonded boards compared with the UF-bonded boards. Superior bonding between the MDI adhesive and the peanut hulls with different particle geometries was also observed; the peanut hull powder and coarse particles were unsuitable for the manufacturing of panels, due to the risk of an internal blowout. The water resistance of the panels was poor, whereas the mechanical strength of the peanut hull particleboard met the class M-1 requirement of the ANSI A208.1 (2009) standard for wood particleboard. C1 [Cheng, Xiyi; He, Xia; Xie, Jie; Quan, Peng; Xu, Kang; Li, Xianjun] Cent South Univ Forestry & Technol, Mat Sci & Engn Coll, Changsha 410004, Hunan, Peoples R China. [Cai, Zhiyong] USDA, Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Li, XJ (reprint author), Cent South Univ Forestry & Technol, Mat Sci & Engn Coll, Changsha 410004, Hunan, Peoples R China. EM lxjmu@163.com FU Hunan Collaborative Innovation Center for High-efficiency Utilization of Wood and Bamboo Resources FX This research work was funded by the Hunan Collaborative Innovation Center for High-efficiency Utilization of Wood and Bamboo Resources. NR 34 TC 0 Z9 0 U1 1 U2 1 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD AUG PY 2016 VL 11 IS 3 BP 7271 EP 7281 DI 10.15376/biores.11.3.7271-7281 PG 11 WC Materials Science, Paper & Wood SC Materials Science GA DY2KV UT WOS:000384922400124 ER PT J AU Essien, C Cheng, QZ Via, BK Loewenstein, EF Wang, XP AF Essien, Charles Cheng, Qingzheng Via, Brian K. Loewenstein, Edward F. Wang, Xiping TI An Acoustics Operations Study for Loblolly Pine (Pinus taeda) Standing Saw Timber with Different Thinning History SO BIORESOURCES LA English DT Article DE Acoustic velocity; Stiffness; Thinning regimes; Loblolly pine ID FLEXURAL PROPERTIES; BENDING STRENGTH; WOOD STIFFNESS; LUMBER; TREES; PLANTATIONS; DENSITY; LOGS; AGE AB There is currently a request from landowners in southeastern USA to provide a nondestructive tool that can differentiate the quality between stands of 25 and 30 years of age subjected to different thinning treatments. A typical site with various thinning regimes was used to vary the wood quality and to determine whether acoustics had the ability to separate for stiffness differences at a given age and local geography. A stand at age 29 with three different spacing (prior thinning) levels was chosen. Three hundred trees (100 per treatment) were randomly selected and acoustically tested for sound velocity using the Time-of-Flight (ToF) method for unthinned, thinned, and twice-thinned stands, respectively. The key finding of the study was that the estimated stiffness of the previously thinned treatments was actually greater than that of the unthinned group, despite having diameters as much as 28% larger. During a forest cruise, knowing that a higher-diameter stand is similar or higher in stiffness could raise the dollar value and harvest priority. C1 [Essien, Charles; Cheng, Qingzheng; Via, Brian K.] Auburn Univ, SFWS, Forest Prod Dev Ctr, 520 Devall Dr, Auburn, AL 36849 USA. [Loewenstein, Edward F.] Auburn Univ, Sch Forestry & Wildlife Sci, 602 Duncan Dr, Auburn, AL 36849 USA. [Wang, Xiping] USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. RP Via, BK (reprint author), Auburn Univ, SFWS, Forest Prod Dev Ctr, 520 Devall Dr, Auburn, AL 36849 USA. EM bkv0003@auburn.edu NR 25 TC 0 Z9 0 U1 1 U2 1 PU NORTH CAROLINA STATE UNIV DEPT WOOD & PAPER SCI PI RALEIGH PA CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA SN 1930-2126 J9 BIORESOURCES JI BioResources PD AUG PY 2016 VL 11 IS 3 BP 7512 EP 7521 DI 10.15376/biores.11.3.7512-7521 PG 10 WC Materials Science, Paper & Wood SC Materials Science GA DY2KV UT WOS:000384922400143 ER PT J AU Maginel, CJ Knapp, BO Kabrick, JM Olson, EK Muzika, RM AF Maginel, Calvin J. Knapp, Benjamin O. Kabrick, John M. Olson, Elizabeth K. Muzika, Rose-Marie TI Floristic Quality Index for woodland ground flora restoration: Utility and effectiveness in a fire-managed landscape SO ECOLOGICAL INDICATORS LA English DT Article DE Prescribed fire; Floristic Quality Index; Coefficient of Conservatism; Ground flora; Missouri Ozarks ID PRESCRIBED FIRE; WETLANDS; FOREST; DIVERSITY; USA; CONSERVATISM; BIODIVERSITY; COEFFICIENTS; DISTURBANCE; ECOSYSTEMS AB Monitoring is a critical component of ecological restoration and requires the use of metrics that are meaningful and interpretable. We analyzed the effectiveness of the Floristic Quality Index (FQI), a vegetative community metric based on species richness and the level of sensitivity to anthropogenic disturbance of individual species present (Coefficient of Conservatism (CC)), using ground layer vegetation data from forests and woodlands with different fire histories in the Missouri Ozarks, USA. Specifically, we used total species richness, mean CC, and FQI to quantify differences in ground layer vegetation between burned and unburned sites, determine if relationships between richness and mean CC were consistent at local and landscape-scales, and evaluate the influence of richness and mean CC on FQI values using empirical data. Concerns regarding FQI identified in previous studies were also observed in this study, including a negative relationship between richness and mean CC. However, we observed this negative relationship using data from all study plots (landscape-scale) but not within discrete site types (local-scale). Relationships among mean CC, richness, and FQI were complicated because species richness was strongly correlated to FQI values across plots in which richness was low, whereas mean CC was only correlated with FQI. values across plots in which richness was high. We conclude that the interpretation of the Floristic Quality Index may be challenged by: (1) the possibility of obtaining the same FQI value through different combinations of mean CC and richness; and (2) the dominating effect of richness on FQI. Although the FQI metric appears responsive to prescribed fire effects on plant communities in the Missouri Ozarks, the inclusion of species richness and mean CC provide more complete indication of plant community response than FQI alone. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Maginel, Calvin J.; Knapp, Benjamin O.; Muzika, Rose-Marie] Univ Missouri, Dept Forestry, 203 ABNR Bldg, Columbia, MO 65211 USA. [Kabrick, John M.] US Forest Serv, USDA, Northern Res Stn, 202 ABNR Bldg, Columbia, MO 65211 USA. [Olson, Elizabeth K.] Ozark Reg Off, Missouri Dept Conservat, 551 Joe Jones Blvd, West Plains, MO 65775 USA. RP Knapp, BO (reprint author), Univ Missouri, Dept Forestry, 203 ABNR Bldg, Columbia, MO 65211 USA. EM knappb@missouri.edu OI Maginel, Calvin/0000-0003-3291-0466 FU University of Missouri School of Natural Resources; Missouri Department of Conservation; Nature Conservancy FX Funding was provided by the University of Missouri School of Natural Resources, the Missouri Department of Conservation, and The Nature Conservancy. Doug Ladd of The Nature Conservancy was generous in offering the data from Chilton Creek for analyses. Lori Thombs and Mark Ellersieck were indispensable in regards to model creation, and additional thanks go to Aaron Stevenson, Candace Galen, Claire Ciafre, and Tesa Madsen-McQueen for their time spent regarding this work. Justin Thomas, Jacob Hadle, and Gabe Waterhouse of the Institute of Botanical Training and the 2013 MOFEP botany crew collected outstanding data, and we are indebted to them for their effort and enjoyment of botany. NR 60 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X EI 1872-7034 J9 ECOL INDIC JI Ecol. Indic. PD AUG PY 2016 VL 67 BP 58 EP 67 DI 10.1016/j.ecolind.2016.02.035 PG 10 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA ED3YV UT WOS:000388785300007 ER PT J AU Holden, ZA Swanson, A Klene, AE Abatzoglou, JT Dobrowski, SZ Cushman, SA Squires, J Moisen, GG Oyler, JW AF Holden, Zachary A. Swanson, Alan Klene, Anna E. Abatzoglou, John T. Dobrowski, Solomon Z. Cushman, Samuel A. Squires, John Moisen, Gretchen G. Oyler, Jared W. TI Development of high-resolution (250 m) historical daily gridded air temperature data using reanalysis and distributed sensor networks for the US Northern Rocky Mountains SO INTERNATIONAL JOURNAL OF CLIMATOLOGY LA English DT Article DE topoclimate; air temperature; cold air drainage; solar radiation; sensor networks; reanalysis ID CONTERMINOUS UNITED-STATES; COMPLEX TERRAIN; SOLAR-RADIATION; CLIMATE DATA; INTERPOLATION; PRECIPITATION; MANAGEMENT; SURFACES; SYSTEM; FOREST AB Gridded temperature data sets are typically produced at spatial resolutions that cannot fully resolve fine-scale variation in surface air temperature in regions of complex topography. These data limitations have become increasingly important as scientists and managers attempt to understand and plan for potential climate change impacts. Here, we describe the development of a high-resolution (250 m) daily historical (1979-2012) temperature data set for the US Northern Rocky Mountains using observations from both long-term weather stations and a dense network of low-cost temperature sensors. Empirically based models for daily minimum and maximum temperature incorporate lapse rates from regional reanalysis data, modelled daily solar insolation and soil moisture, along with time invariant canopy cover and topographic factors. Daily model predictions demonstrate excellent agreement with independent observations, with mean absolute errors of <1.4 degrees C for both minimum and maximum temperature. Topographically resolved temperature data may prove useful in a range of applications related to hydrology, fire regimes and fire behaviour, and habitat suitability modelling. The form of the models may provide a means for downscaling future temperature scenarios that account for potential fine-scale topographically mediated changes in near-surface temperature. C1 [Holden, Zachary A.] USDA Forest Serv Reg 1, 200 East Broadway St, Missoula, MT 50807 USA. [Holden, Zachary A.; Swanson, Alan; Klene, Anna E.] Univ Montana, Dept Geog, Missoula, MT 59812 USA. [Abatzoglou, John T.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. [Dobrowski, Solomon Z.] Univ Montana, Coll Forestry, Missoula, MT 59812 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ USA. [Squires, John] US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA. [Moisen, Gretchen G.] US Forest Serv, Rocky Mt Res Stn, Ogden, UT USA. [Oyler, Jared W.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Holden, ZA (reprint author), USDA Forest Serv Reg 1, 200 East Broadway St, Missoula, MT 50807 USA. EM zaholden@fs.fed.us OI Abatzoglou, John/0000-0001-7599-9750 FU US Forest Service [10-CS-11015600-007]; University of Montana [10-CS-11015600-007]; NASA Applied Science Program - Wildland Fire award [NNH11ZDA001N-FIRES]; JS; Interior West Forest Inventory and Analysis program; National Science Foundation (DEB) [1145985] FX This work was funded by USFS Region 1 Fire and Aviation Management through a Challenge Cost-Share agreement between the US Forest Service and the University of Montana (agreement no. 10-CS-11015600-007) and through a NASA Applied Science Program - Wildland Fire award (agreement number NNH11ZDA001N-FIRES). Additional funding was provided by JS. Support was also provided by the Interior West Forest Inventory and Analysis program. Additional support for SZD and ZAH was provided by the National Science Foundation (DEB; 1145985). We gratefully acknowledge the many ecologists and field technicians who distributed and retrieved temperature sensors used in this study including Jessica Page, Jeff DiBenedetto, John Tsroka of the Clark Fork Coalition, and technicians at the Idaho Bird Observatory. We thank Stephan Pracht and Brian Holden for managing field operations and distributing and collecting sensors. The authors acknowledge the Texas Advanced Computing Center (TACC) at The University of Texas at Austin for providing HPC resources that have contributed to the research results reported within this paper (http://www.tacc.utexas.edu). The authors declare no conflict of interest. NR 54 TC 2 Z9 2 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0899-8418 EI 1097-0088 J9 INT J CLIMATOL JI Int. J. Climatol. PD AUG PY 2016 VL 36 IS 10 BP 3620 EP 3632 DI 10.1002/joc.4580 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DW4LE UT WOS:000383613700016 ER PT J AU Madsen, MD Davies, KW Boyd, CS Kerby, JD Svejcar, TJ AF Madsen, Matthew D. Davies, Kirk W. Boyd, Chad S. Kerby, Jay D. Svejcar, Tony J. TI Emerging seed enhancement technologies for overcoming barriers to restoration SO RESTORATION ECOLOGY LA English DT Review DE annual grasses; restoration; revegetation; seed coating; seed technology; wildfire ID SOIL-WATER REPELLENCY; RANGELAND DEGRADATION; SAGEBRUSH STEPPE; ACTIVATED CARBON; GRASS; ESTABLISHMENT; REVEGETATION; RECRUITMENT; VEGETATION; HERBICIDES AB Rangelands occupy over a third of global land area, and in many cases are in less than optimum condition as a result of past land use, catastrophic wildfire, and other disturbances, invasive species, or climate change. Often the only means of restoring these lands involves seeding desirable species, yet there are few cost effective-seeding technologies, especially for the more arid rangeland types. The inability to consistently establish desired plants from seed may indicate that seeding technologies being employed are not successful in addressing the primary sources of mortality in the progression from seed to established plant. Seed enhancement technologies allow for the physical manipulation and application of materials to the seed that can enhance germination, emergence, and/or early seedling growth. In this article, we examine some of the major limiting factors impairing seedling establishment in North America's sagebrush steppe ecosystem and propose seed enhancement technologies that may have the potential to overcome these restoration barriers. We discuss specific technologies for: (1) increasing soil water availability; (2) enhancing seedling emergence in crusting soil; (3) controlling the timing of seed germination; (4) improving plantability and emergence of small-seeded species; (5) enhancing seed coverage of broadcasted seeds; and (6) protecting seedlings from pre-emergent herbicide. Concepts and technologies in this article for restoring the sagebrush steppe ecosystem may apply generally to semiarid and arid rangelands around the globe. C1 [Madsen, Matthew D.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Davies, Kirk W.; Boyd, Chad S.; Svejcar, Tony J.] ARS, USDA, Eastern Oregon Agr Res Ctr, Burns, OR USA. [Kerby, Jay D.] Nat Conservancy Burns, Burns, OR USA. RP Madsen, MD (reprint author), Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. EM matthew.madsen@byu.edu FU BHP Billiton Iron Ore Community Develop Project of the Restoration Seedbank Initiative [8600048550]; Aquatrols Corporation of America; Nature Conservancy; Priscilla Bullitt-Collins Northwest Conservation Fund; Kane and Two Mile Research and Stewardship Partnership; USDA-ARS FX This article is a direct outcome of discussions held during the Restoration Seedbank Initiative's Think Tank Workshop focussing on "seed enablement technologies and recreating the recruitment niche in degraded environments." Logistical and financial support was provided by a BHP Billiton Iron Ore Community Develop Project (contract no. 8600048550) under the auspices of the Restoration Seedbank Initiative, a partnership between BHP Billiton Iron Ore, The University of Western Australia, and the Botanic Gardens and Parks Authority. Funding was also provided by Aquatrols Corporation of America, The Nature Conservancy, The Priscilla Bullitt-Collins Northwest Conservation Fund, Kane and Two Mile Research and Stewardship Partnership, and the USDA-ARS. Mention of a proprietary product does not constitute a guarantee or warranty of the product by USDA or the authors and does not imply its approval to the exclusion of the other products that also may be suitable. USDA is an equal opportunity provider and employer. NR 59 TC 5 Z9 5 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD AUG PY 2016 VL 24 SU 2 SI SI BP S77 EP S84 DI 10.1111/rec.12332 PG 8 WC Ecology SC Environmental Sciences & Ecology GA DW5YK UT WOS:000383723400005 ER PT J AU Chen, ZC Griffis, TJ Millet, DB Wood, JD Lee, X Baker, JM Xiao, K Turner, PA Chen, M Zobitz, J Wells, KC AF Chen, Zichong Griffis, Timothy J. Millet, Dylan B. Wood, Jeffrey D. Lee, Xuhui Baker, John M. Xiao, Ke Turner, Peter A. Chen, Ming Zobitz, John Wells, Kelley C. TI Partitioning N2O emissions within the US Corn Belt using an inverse modeling approach SO GLOBAL BIOGEOCHEMICAL CYCLES LA English DT Article ID NITROUS-OXIDE EMISSIONS; ATMOSPHERIC METHANE; GENERALIZED-MODEL; STILT MODEL; TALL TOWER; CONSTRAINTS; STREAM; SOILS; PARAMETERIZATION; DENITRIFICATION AB Nitrous oxide (N2O) emissions within the US Corn Belt have been previously estimated to be 200-900% larger than predictions from emission inventories, implying that one or more source categories in bottom-up approaches are underestimated. Here we interpret hourly N2O concentrations measured during 2010 and 2011 at a tall tower using a time-inverted transport model and a scale factor Bayesian inverse method to simultaneously constrain direct and indirect agricultural emissions. The optimization revealed that both agricultural source categories were underestimated by the Intergovernmental Panel on Climate Change (IPCC) inventory approach. However, the magnitude of the discrepancies differed substantially, ranging from 42 to 58% and from 200 to 525% for direct and indirect components, respectively. Optimized agricultural N2O budgets for the Corn Belt were 319 +/- 184 (total), 188 +/- 66 (direct), and 131 +/- 118 Gg N yr(-1) (indirect) in 2010, versus 471 +/- 326, 198 +/- 80, and 273 +/- 246 Gg N yr(-1) in 2011. We attribute the interannual differences to varying moisture conditions, with increased precipitation in 2011 amplifying emissions. We found that indirect emissions represented 41-58% of the total agricultural budget, a considerably larger portion than the 25-30% predicted in bottom-up inventories, further highlighting the need for improved constraints on this source category. These findings further support the hypothesis that indirect emissions are presently underestimated in bottom-up inventories. Based on our results, we suggest an indirect emission factor for runoff and leaching ranging from 0.014 to 0.035 for the Corn Belt, which represents an upward adjustment of 1.9-4.6 times relative to the IPCC and is in agreement with recent bottom-up field studies. C1 [Chen, Zichong; Griffis, Timothy J.; Millet, Dylan B.; Wood, Jeffrey D.; Baker, John M.; Xiao, Ke; Turner, Peter A.; Chen, Ming; Wells, Kelley C.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Lee, Xuhui] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Baker, John M.] ARS, USDA, St Paul, MN USA. [Zobitz, John] Augsburg Coll, Dept Math, Minneapolis, MN USA. RP Chen, ZC (reprint author), Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. EM chen3274@umn.edu RI Millet, Dylan/G-5832-2012 FU United States Department of Agriculture [USDA-NIFA 2013-67019-21364]; USDA-ARS, NOAA [NA13OAR4310086]; MnDRIVE FX Financial support for this research has been provided by the United States Department of Agriculture, grant USDA-NIFA 2013-67019-21364; USDA-ARS, NOAA (grant NA13OAR4310086); and MnDRIVE. We express our sincere thanks to Tom Nelson and Minnesota Public Radio for the logistical support at the KCMP tall tower. All models and analyses were performed at the Minnesota Supercomputing Institute for Advanced Computational Research. Data are hosted at http://www.biometeorology.umn.edu/research/data-archives. NR 58 TC 1 Z9 1 U1 10 U2 10 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0886-6236 EI 1944-9224 J9 GLOBAL BIOGEOCHEM CY JI Glob. Biogeochem. Cycle PD AUG PY 2016 VL 30 IS 8 BP 1192 EP 1205 DI 10.1002/2015GB005313 PG 14 WC Environmental Sciences; Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Geology; Meteorology & Atmospheric Sciences GA EC9IG UT WOS:000388457500005 ER PT J AU Baloch, AM Baloch, AW Liu, S Gao, P Baloch, MJ Wang, XZ Davis, AR Ali, M Luan, FS AF Baloch, Abdul Majeed Baloch, Abdul Wahid Liu, Shi Gao, Peng Baloch, M. Jurial Wang, Xuezhng Davis, Angela R. Ali, M. Luan, Feishi TI LINKAGE MAP CONSTRUCTION AND QTL ANALYSIS OF FRUIT TRAITS IN MELON (CUCUMIS MELO L.) BASED ON CAPS MARKERS SO PAKISTAN JOURNAL OF BOTANY LA English DT Article DE Linkage map; CAPS marker; QTLs; Melon ID MOLECULAR MARKERS; GENETIC-MAP; RESISTANCE; DISEASE; MAIZE; LOCI AB In the current experiment, the quantitative trait loci (QTL) analysis was done by composite interval mapping method to detect QTLs in edge, central parts and fruit shape of melon. In this context, 235 F-2 populations along with their parents were evaluated for fruit size, shape and color under replicated trail at Horticulture Experimental Station of Northeast Agricultural University, Harbin, China, during the growing year 2014. Moreover, 96 pairs of CAPS markers were used to construct a linkage map using F2 population that was derived from the cross between two contrasting parents (MR-1 and Topmark). The total length of linkage map was found to be 4984.1cM with an average of 51.9177 cM between the markers. In a total, we detected ten QTLs, in which one was major, while others were minor. Five QTLs were detected in the edge part of melon fruit and three QTLs were detected in central parts of melon and all were considered as Brix content. Two QTLs were related with fruit shape, Our present genetic and QTLs mapping would be proved useful in plant breeding programs for the improvement of economically important horticultural traits. C1 [Baloch, Abdul Majeed; Liu, Shi; Gao, Peng; Wang, Xuezhng; Luan, Feishi] Northeast Agr Univ, Coll Hort, Harbin, Peoples R China. [Baloch, Abdul Wahid; Baloch, M. Jurial] Sindh Agr Univ Tandojam, Dept Plant Breeding & Genet, Tandojam, Pakistan. [Davis, Angela R.] ARS, South Cent Agr Res Lab, USDA, Davis, CA 95618 USA. [Davis, Angela R.] HM Clause, 9241 Mace Blvd, Davis, CA 95618 USA. [Ali, M.] Sindh Agr Univ Tandojam, Dept Biotechnol, Tandojam, Pakistan. RP Luan, FS (reprint author), Northeast Agr Univ, Coll Hort, Harbin, Peoples R China. EM luanfeishi@sina.com FU National Nature Science Foundation of China [31272186] FX This research was supported by the National Nature Science Foundation of China (31272186). And China Agriculture Research System (CARS-26-02) NR 28 TC 0 Z9 0 U1 1 U2 1 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 EI 2070-3368 J9 PAK J BOT JI Pak. J. Bot. PD AUG PY 2016 VL 48 IS 4 BP 1579 EP 1584 PG 6 WC Plant Sciences SC Plant Sciences GA EB9SN UT WOS:000387737500032 ER PT J AU Berryman, E Ryan, MG Bradford, JB Hawbaker, TJ Birdsey, R AF Berryman, E. Ryan, M. G. Bradford, J. B. Hawbaker, T. J. Birdsey, R. TI Total belowground carbon flux in subalpine forests is related to leaf area index, soil nitrogen, and tree height SO ECOSPHERE LA English DT Article DE carbon dioxide; carbon sequestration; respiration; soil respiration ID HYDRAULIC LIMITATION HYPOTHESIS; POSTFIRE LODGEPOLE PINE; MAINTENANCE RESPIRATION; EUCALYPTUS PLANTATION; ECOSYSTEM RESPIRATION; NORTH-AMERICA; AGE SEQUENCE; STAND AGE; TAEDA L.; ALLOCATION AB In forests, total belowground carbon (C) flux (TBCF) is a large component of the C budget and represents a critical pathway for delivery of plant C to soil. Reducing uncertainty around regional estimates of forest C cycling may be aided by incorporating knowledge of controls over soil respiration and TBCF. Photosynthesis, and presumably TBCF, declines with advancing tree size and age, and photosynthesis increases yet C partitioning to TBCF decreases in response to high soil fertility. We hypothesized that these causal relationships would result in predictable patterns of TBCF, and partitioning of C to TBCF, with natural variability in leaf area index (LAI), soil nitrogen (N), and tree height in subalpine forests in the Rocky Mountains, USA. Using three consecutive years of soil respiration data collected from 22 0.38-ha locations across three 1-km(2) subalpine forested landscapes, we tested three hypotheses: (1) annual soil respiration and TBCF will show a hump-shaped relationship with LAI; (2) variability in TBCF unexplained by LAI will be related to soil nitrogen (N); and (3) partitioning of C to TBCF (relative to woody growth) will decline with increasing soil N and tree height. We found partial support for Hypothesis 1 and full support for Hypotheses 2 and 3. TBCF, but not soil respiration, was explained by LAI and soil N patterns (r(2) = 0.49), and the ratio of annual TBCF to TBCF plus aboveground net primary productivity (ANPP) was related to soil N and tree height (r(2) = 0.72). Thus, forest C partitioning to TBCF can vary even within the same forest type and region, and approaches that assume a constant fraction of TBCF relative to ANPP may be missing some of this variability. These relationships can aid with estimates of forest soil respiration and TBCF across landscapes, using spatially explicit forest data such as national inventories or remotely sensed data products. C1 [Berryman, E.; Hawbaker, T. J.] US Geol Survey, Geosci & Environm Change Sci Ctr, Denver, CO 80225 USA. [Ryan, M. G.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Ryan, M. G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80521 USA. [Bradford, J. B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86011 USA. [Birdsey, R.] US Forest Serv, Northern Res Stn, Newtown Sq, PA 19073 USA. RP Berryman, E (reprint author), US Geol Survey, Geosci & Environm Change Sci Ctr, Denver, CO 80225 USA. EM eberryman@usgs.gov RI Ryan, Michael/A-9805-2008; Bradford, John/E-5545-2011; OI Ryan, Michael/0000-0002-2500-6738; BERRYMAN, ERIN/0000-0001-8699-2474 FU USFS North American Carbon Program; USGS Climate and Land Use Change Mission Area; NASA [CARBON/04-0225-0191] FX The USFS North American Carbon Program and the USGS Climate and Land Use Change Mission Area funded this work. Data collection was funded by NASA grant CARBON/04-0225-0191. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. The authors acknowledge the efforts of Brianna Miles for data collection, as well as Jennifer Briggs (USGS) and anonymous peer reviewers whose comments greatly improved the quality of this manuscript. NR 68 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD AUG PY 2016 VL 7 IS 8 AR e01418 DI 10.1002/ecs2.1418 PG 16 WC Ecology SC Environmental Sciences & Ecology GA EB2RG UT WOS:000387208900012 ER PT J AU Ladin, ZS D'Amico, V Baetens, JM Roth, RR Shriver, WG AF Ladin, Zachary S. D'Amico, Vincent Baetens, Jan M. Roth, Roland R. Shriver, W. Gregory TI Long-term dynamics in local host-parasite interactions linked to regional population trends SO ECOSPHERE LA English DT Article DE brood parasitism; Brown-headed Cowbird; host-parasite interactions; Hylocichla mustelina; Molothrus ater; population dynamics; Wood Thrush ID BROWN-HEADED COWBIRDS; CONSPECIFIC BROOD PARASITISM; WOOD-THRUSH; HABITAT QUALITY; NESTLING COMPETITION; SEASONAL FECUNDITY; MIGRATORY SONGBIRD; PASSERINE BIRDS; MOLOTHRUS-ATER; PREDATION AB Temporal changes in the relative abundances of host-parasite populations can influence the magnitude of the effects of corresponding interspecific interactions. When parasite populations are at relatively low abundance, the negative effects on host populations may be insignificant, but when parasite abundance increases beyond critical thresholds, they can have population limiting effects on the host. Here, we used data from a 40-yr demographic study on breeding Wood Thrushes (Hylocichla mustelina) and avian brood parasitic Brown-headed Cowbirds (Molothrus ater) in the mid-Atlantic United States to disentangle host-parasite interactions. The relative abundance for these two species has changed both locally and regionally over this time period with a reduction in host abundance coincident with an increase in the parasite population. We detected a fivefold increase in Brown-headed Cowbird parasitism rates of Wood Thrushes over the 40-yr time period leading to a reduction in Wood Thrush fitness (i.e.,adult survival, fecundity, and recruitment). After accounting for the effects of Wood Thrush age, individual, and annual and within-season variation in reproduction, we found that Wood Thrushes exhibited increased reproductive effort (produced more nests per year) as nest parasitism rates increased. Additionally, we found that as parasitism rates increased, both Wood Thrush clutch size and fecundity declined. In conjunction with widespread habitat loss and land use change on both wintering and breeding ranges, increasing rates of Brown-headed Cowbird parasitism are reducing Wood Thrush fitness, and are likely contributing to observed regional Wood Thrush population declines. Coordinated local and regional efforts to reduce Brown-headed Cowbird populations, particularly in fragmented landscapes, may help reduce the decline for Wood Thrushes, and likely other parasitized Neotropical migratory species. C1 [Ladin, Zachary S.] Univ Delaware, Dept Entomol & Wildlife Ecol, 264 Townsend Hall, Newark, DE 19716 USA. [D'Amico, Vincent] US Forest Serv, Northern Res Stn, 531 South Coll Ave,Townsend Hall, Newark, DE 19716 USA. [Baetens, Jan M.] Univ Ghent, Dept Math Modeling Stat & Bioinformat, Coupure Links 653, B-9000 Ghent, Belgium. [Roth, Roland R.; Shriver, W. Gregory] Univ Delaware, Dept Entomol & Wildlife Ecol, 250 Townsend Hall, Newark, DE 19716 USA. RP Ladin, ZS (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, 264 Townsend Hall, Newark, DE 19716 USA. EM zach@udel.edu FU U.S. Department of Agriculture McIntire-Stennis Forestry Research Program, U.S. Forest Service; University of Delaware FX We thank funding sources from the U.S. Department of Agriculture McIntire-Stennis Forestry Research Program, U.S. Forest Service, and the University of Delaware. We are grateful for cooperation from the University of Delaware for access to the study area. We thank the following contributors for their involvement in field work and data collection for this study: S. Adalsteinnson, J. Buler, D. Ecker, D. Greene, N. Hengst, S. Mkheidze, K. Pastirik, C. Rega, K. Serno, and M. Walker. NR 88 TC 0 Z9 0 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD AUG PY 2016 VL 7 IS 8 AR e01420 DI 10.1002/ecs2.1420 PG 13 WC Ecology SC Environmental Sciences & Ecology GA EB2RG UT WOS:000387208900014 ER PT J AU Walters, MB Farinosi, EJ Willis, JL Gottschalk, KW AF Walters, Michael B. Farinosi, Evan J. Willis, John L. Gottschalk, Kurt W. TI Managing for diversity: harvest gap size drives complex light, vegetation, and deer herbivory impacts on tree seedlings SO ECOSPHERE LA English DT Article DE deer browsing; deer herbivory; gap partitioning; harvest gaps; northern hardwood forest; plant competition; shade tolerance; tree diversity; tree seedling growth; tree seedling survival; uneven-aged management; white-tailed deer ID NORTHERN HARDWOOD FOREST; LATE-SUCCESSIONAL FORESTS; UPPER GREAT-LAKES; SPECIES-DIVERSITY; OLD-GROWTH; GROUP-SELECTION; YELLOW BIRCH; SUGAR MAPLE; HEMLOCK SEEDLINGS; TEMPERATE FOREST AB Many managed northern hardwood forests are characterized by low-diversity tree regeneration. Small harvest gaps, competition from shrub-herb vegetation, and browsing by white-tailed deer (Odocoileus virginianus) contribute to this pattern, but we know little about how these factors interact. With a stand-scale experiment, we examined the effects of gap size (0-3234 m(2)), vegetation (weeded: unweeded), and deer (fenced: unfenced) on seedling growth and survival for 18 tree species. With increasing gap size and light, shrub-herb vegetation density increased, while deer browsing on seedlings in unweeded plots decreased. Fenced: weeded seedlings of all species increased in height up to 35-45% light, with optimal growth in large-group selection and patch cut harvest gaps. Height growth rank order among tree species changed between gap sizes, but growth varied little in small, low-light gaps. Instead, a low-light survival (i.e., shade tolerance) vs. high-light growth tradeoff we observed is likely more important for species sorting of gap sizes. Shrub-herb vegetation decreased seedling survival and growth, especially in larger harvest gaps, shifting gap size optima to smaller gaps, but had little effect on growth/survival rank order among species. In contrast, deer had strong impacts on growth rank order, especially in larger gaps where species differences in growth potential were trumped by differences in deer browsing pressure responses. However, contrary to their consistently negative main effects, vegetation and deer had two positive interacting effects: dense shrub-herb vegetation in large gaps protected seedlings of faster-growing species from browsing and deer browsing of shrub-herb vegetation modestly increased light and growth of short, suppressed, browsing-avoided species. In summary, harvest gap size-mediated light availability, shrub-herb vegetation, and deer herbivory had strong interacting effects on tree seedling interspecific performance ranks and intraspecific optimal gap sizes. For management, a broad range of harvest gap sizes and rapid establishment of tree regeneration (naturally or planted) to minimize shrub-herb competition should increase tree diversity in forests with few deer. However, with deer browsing pressure, a more limited set of lesser-browsed species are likely to recruit successfully regardless of gap size, except in large patch cut gaps, where recruitment of faster-growing, shade-intolerant species is possible. C1 [Walters, Michael B.; Farinosi, Evan J.; Willis, John L.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Gottschalk, Kurt W.] Forest Serv, USDA, Northern Res Stn, Morgantown, WV 26505 USA. [Willis, John L.] Mississippi State Univ, Dept Forestry, Mississippi State, MS 39762 USA. RP Walters, MB (reprint author), Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. EM mwalters@msu.edu FU Michigan Department of Natural Resources, Forest Management Division; USDA Forest Service FX The authors are extremely thankful to the Michigan Department of Natural Resources, Forest Management Division. In addition to providing most of the funding for the project, they were important cooperators involved in every aspect of the study. Among others, David Neumann, Ron Murray, and the Management Team were of great help. We also thank Fred Prince Jr. (1925-2011) and Forests for the Future for getting us started on this project at the Levering site and for the support of the USDA Forest Service. The remaining authors of this report were saddened by the passing of Kurt Gottschalk just prior to the original submission of this report. He was a great colleague, and we are fortunate to have spent time with him in the beautiful hardwood forests of Northern Michigan and philosophizing over beers at Clyde's. Rest well Kurt. NR 72 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD AUG PY 2016 VL 7 IS 8 AR e01397 DI 10.1002/ecs2.1397 PG 29 WC Ecology SC Environmental Sciences & Ecology GA EB2RG UT WOS:000387208900004 ER PT J AU Pellerin, BA Stauffer, BA Young, DA Sullivan, DJ Bricker, SB Walbridge, MR Clyde, GA Shaw, DM AF Pellerin, Brian A. Stauffer, Beth A. Young, Dwane A. Sullivan, Daniel J. Bricker, Suzanne B. Walbridge, Mark R. Clyde, Gerard A., Jr. Shaw, Denice M. TI EMERGING TOOLS FOR CONTINUOUS NUTRIENT MONITORING NETWORKS: SENSORS ADVANCING SCIENCE AND WATER RESOURCES PROTECTION SO JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION LA English DT Article DE sensors; nutrients; water quality; information management ID HARMFUL ALGAL BLOOMS; SPRING-FED RIVER; IN-SITU SENSORS; MISSISSIPPI RIVER; HIGH-RESOLUTION; DRINKING-WATER; WASTE-WATER; DATA DELUGE; NITRATE; FREQUENCY AB Sensors and enabling technologies are becoming increasingly important tools for water quality monitoring and associated water resource management decisions. In particular, nutrient sensors are of interest because of the well-known adverse effects of nutrient enrichment on coastal hypoxia, harmful algal blooms, and impacts to human health. Accurate and timely information on nutrient concentrations and loads is integral to strategies designed to minimize risk to humans and manage the underlying drivers of water quality impairment. Using nitrate sensors as the primary example, we highlight the types of applications in freshwater and coastal environments that are likely to benefit from continuous, real-time nutrient data. The concurrent emergence of new tools to integrate, manage, and share large datasets is critical to the successful use of nutrient sensors and has made it possible for the field of continuous monitoring to rapidly move forward. We highlight several near-term opportunities for federal agencies, as well as the broader scientific and management community, that will help accelerate sensor development, build and leverage sites within a national network, and develop open data standards and data management protocols that are key to realizing the benefits of a large-scale, integrated monitoring network. Investing in these opportunities will provide new information to guide management and policies designed to protect and restore our nation's water resources. C1 [Pellerin, Brian A.] US Geol Survey, Calif Water Sci Ctr, 6000 J St,Placer Hall, Sacramento, CA 95819 USA. [Stauffer, Beth A.] Univ Louisiana Lafayette, Dept Biol, Lafayette, LA 70504 USA. [Young, Dwane A.] US EPA, Off Water, Washington, DC 20460 USA. [Sullivan, Daniel J.] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI 53562 USA. [Bricker, Suzanne B.] NOAA, Natl Ctr Coastal Ocean Sci, Silver Spring, MD 20910 USA. [Walbridge, Mark R.] ARS, USDA, Beltsville, MD 20705 USA. [Clyde, Gerard A., Jr.] US Army Corp Engineers, Tulsa Dist, Tulsa, OK 74128 USA. [Shaw, Denice M.] US EPA, Off Res & Dev, Washington, DC 20460 USA. RP Pellerin, BA (reprint author), US Geol Survey, Calif Water Sci Ctr, 6000 J St,Placer Hall, Sacramento, CA 95819 USA. EM bpeller@usgs.gov FU USGS Office of Water Quality; USGS National Water Quality Assessment Program; National Water Quality Monitoring Council FX The authors benefited from insights and comments from Robert Gilliom, Charles Crawford, Donna Myers, Bill Wilber, Gary Rowe, Pixie Hamilton, Mark Nilles, Andy Ziegler, Jeff Lape, and Richard Mitchell. We thank Pete Penoyer (National Park Service) and two anonymous reviewers for helpful comments on the manuscript. This article was supported by the USGS Office of Water Quality, the USGS National Water Quality Assessment Program, and the National Water Quality Monitoring Council. NR 81 TC 1 Z9 1 U1 11 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1093-474X EI 1752-1688 J9 J AM WATER RESOUR AS JI J. Am. Water Resour. Assoc. PD AUG PY 2016 VL 52 IS 4 BP 993 EP 1008 DI 10.1111/1752-1688.12386 PG 16 WC Engineering, Environmental; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA EB2DW UT WOS:000387168800016 ER PT J AU Marcati, CR Machado, SR Podadera, DS de Lara, NOT Bosio, F Wiedenhoeft, AC AF Marcati, Carmen R. Machado, Silvia R. Podadera, Diego Sotto Totti de lara, Natalia O. Bosio, Fabio Wiedenhoeft, Alex C. TI Cambial activity in dry and rainy season on branches from woody species growing in Brazilian Cerrado SO FLORA LA English DT Article DE Budding; Cambium; Savanna-like ecosystem; Photoperiod; Precipitation; Temperature ID FOREST TREES; TROPICAL TREES; GROWTH PERIODICITY; VASCULAR CAMBIUM; SECONDARY XYLEM; SAVANNA TREES; ANNUAL RHYTHM; PHENOLOGY AB Seasonal cambial activity was investigated in one-to three-year-old branch modules (branch constructional units) of ten woody species from cerrado sensu stricto, a savanna-like ecosystem, of southern Brazil. Relationships between cambial activity and environmental factors (precipitation, temperature, day length) and leaf production were tested using generalized linear mixed model. Regardless the plant habit and leaf shedding patterns, cambial activity (about 5 months) corresponded to the core of the rainy season (when at least 66% of the annual precipitation had been reached), even though there was some delay or advance in the timing of cambial activity depending on the branch age or species. Cambial activity began in the dry season and ceased before the end of rainy season. Although cambial activity has been positively correlated with day length, precipitation, temperature, and budding for all species, day length, among the assessed factors, seems to be the best predictor of onset and cessation of cambial activity in the branch modules of cerrado species. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Marcati, Carmen R.] Univ Estadual Paulista UNESP, Fac Ciencias Agron, Dept Ciencia Florestal, Rua Jose Barbosa Barros 1780, BR-18610307 Botucatu, SP, Brazil. [Machado, Silvia R.; Wiedenhoeft, Alex C.] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Bot, Botucatu, SP, Brazil. [Podadera, Diego Sotto] Univ Estadual Campinas, Programa Posgrad Ecol, Campinas, SP, Brazil. [Totti de lara, Natalia O.; Bosio, Fabio] UNESP, Programa Posgrad Ciencias Biol Bot, Botucatu, SP, Brazil. [Wiedenhoeft, Alex C.] USDA, Forest Prod Lab, Ctr Wood Anat Res, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Marcati, CR (reprint author), Univ Estadual Paulista UNESP, Fac Ciencias Agron, Dept Ciencia Florestal, Rua Jose Barbosa Barros 1780, BR-18610307 Botucatu, SP, Brazil. EM carmen@fca.unesp.bi RI Marcati, Carmen/C-6490-2012; Podadera, Diego/C-9010-2014; OI Podadera, Diego/0000-0002-1381-6645; Marcati, Carmen/0000-0001-5723-6450 FU Pro-Reitoria de Pos-Graduacao, Univ Estadual Paulista (PROPG, UNESP) [003/2009]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [301352/2008-9, 302657/2011-8]; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2003/13579-5, 2003/13578-9, 2009/17778-9] FX This work was supported by Pro-Reitoria de Pos-Graduacao, Univ Estadual Paulista (PROPG, UNESP) (Edital no 003/2009), the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq Proc. 301352/2008-9) and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP Procs. 2003/13579-5, 2003/13578-9, 2009/17778-9) to the first author. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq Proc. 302657/2011-8) for research scholarship to S.R. Machado. We thank Dr. Ray Franklin Evert for his help and assistance in material examination, and Anselmo Nogueira and Claudia Franca Barros for assistance in statistical analysis. Dr. Linda Styer Caldas (in memoriam) and Vera T. R. Coradin for advice in collecting the samples and conducting the research. Julia Sonsin Oliveira and Clemente Jose Campos provided assistance in the field. Francely Ap. M. de Tillio, Liliane C. Pereira, Yve Canaveze, Suzana B. Barbosa, Rafaela P. Parmeggiani, Clivia C. F. Possobom, and Inara R. Moreira-Coneglian provided valuable technical assistance in the laboratory. Thanks also to the USDA Forest Products Laboratory for general support. We used "first-last-author-emphasis", and the sequence of other authors reflects the declining importance of their contribution. NR 52 TC 0 Z9 0 U1 8 U2 8 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0367-2530 EI 1618-0585 J9 FLORA JI Flora PD AUG PY 2016 VL 223 BP 1 EP 10 DI 10.1016/j.flora.2016.04.008 PG 10 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA EA8EK UT WOS:000386867900001 ER PT J AU Wei, W Chen, D Wang, LX Daryanto, S Chen, LD Yu, Y Lu, YL Sun, G Feng, TJ AF Wei, Wei Chen, Die Wang, Lixin Daryanto, Stefani Chen, Liding Yu, Yang Lu, Yonglong Sun, Ge Feng, Tianjiao TI Global synthesis of the classifications, distributions, benefits and issues of terracing SO EARTH-SCIENCE REVIEWS LA English DT Review DE Terracing; Ecosystem services; Worldwide distribution; Land degradation; Food security ID IFUGAO RICE TERRACES; SOIL-EROSION; AGRICULTURAL TERRACES; BENCH TERRACES; WATER CONSERVATION; LAND-USE; MANAGEMENT-PRACTICES; PADDY FIELDS; SEMIARID SHRUBLANDS; VINEYARD PLANTATION AB For thousands of years, humans have created different types of terraces in different sloping conditions, meant to mitigate flood risks, reduce soil erosion and conserve water. These anthropogenic landscapes can be found in tropical and subtropical rainforests, deserts, and arid and semiarid mountains across the globe. Despite the long history, the roles of and the mechanisms by which terracing improves ecosystem services (ESs) remain poorly understood. Using literature synthesis and quantitative analysis, the worldwide types, distributions, major benefits and issues of terracing are presented in this review. A key terracing indicator, defined as the ratio of different ESs under terraced and non-terraced slopes (delta), was used to quantify the role of terracing in providing ESs. Our results indicated that ESs provided by terracing was generally positive because the mean values of delta were mostly greater than one. The most prominent role of terracing was found in erosion control (11.46 +/- 2.34), followed by runoff reduction (2.60 +/- 1.79), biomass accumulation (1.94 +/- 0.59), soil water recharge (1.20 +/- 0.23), and nutrient enhancement (1.20 +/- 0.48). Terracing, to a lesser extent, could also enhance the survival rates of plant seedlings, promote ecosystem restoration, and increase crop yields. While slopes experiencing severe human disturbance (e.g., overgrazing and deforestation) can generally become more stable after terracing, negative effects of terracing may occur in poorly-designed or poorly-managed terraces. Among the reasons are the lack of environmental legislation, changes in traditional concepts and lifestyles of local people, as well as price decreases for agricultural products. All of these can accelerate terrace abandonment and degradation. In light of these findings, possible solutions regarding socio-economic changes and techniques to improve already degraded terraces are discussed. (C) 2016 Elsevier B.V. All rights reserved. C1 [Wei, Wei; Chen, Die; Chen, Liding; Yu, Yang; Lu, Yonglong; Feng, Tianjiao] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China. [Chen, Die; Feng, Tianjiao] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Wei, Wei; Wang, Lixin; Daryanto, Stefani] Indiana Univ Purdue Univ Indianapolis, Dept Earth Sci, Indianapolis, IN 46202 USA. [Sun, Ge] US Forest Serv, Southern Res Stn, USDA, Raleigh, NC 27606 USA. RP Chen, LD (reprint author), 18 Shuangqing Rd, Beijing 100085, Peoples R China. EM liding@rcees.ac.cn FU National Natural Science Foundation of China [41390462, 41371123]; Innovation Project of the State Key Laboratory of Urban and Regional Ecology of China [SKLURE2013-1-02]; USDA [2014-51130-22492] FX This research was supported by the National Natural Science Foundation of China (41390462; 41371123), and the Innovation Project of the State Key Laboratory of Urban and Regional Ecology of China (SKLURE2013-1-02). LW acknowledges support from USDA grant (2014-51130-22492). Our sincere thanks to Dr. Tom Hinckley, the editor Dr. Joan Florsheim, as well as the anonymous reviewers for their constructive comments and kind assistance. NR 143 TC 1 Z9 1 U1 26 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0012-8252 EI 1872-6828 J9 EARTH-SCI REV JI Earth-Sci. Rev. PD AUG PY 2016 VL 159 BP 388 EP 403 DI 10.1016/j.earscirev.2016.06.010 PG 16 WC Geosciences, Multidisciplinary SC Geology GA DZ9HT UT WOS:000386186600021 ER PT J AU Harshman, SG Fu, XY Karl, JP Barger, K Lamon-Fava, S Kuliopulos, A Greenberg, AS Smith, D Shen, XH Booth, SL AF Harshman, Stephanie G. Fu, Xueyan Karl, J. Philip Barger, Kathryn Lamon-Fava, Stefania Kuliopulos, Athan Greenberg, Andrew S. Smith, Donald Shen, Xiaohua Booth, Sarah L. TI Tissue Concentrations of Vitamin K and Expression of Key Enzymes of Vitamin K Metabolism Are Influenced by Sex and Diet but Not Housing in C57Bl6 Mice SO JOURNAL OF NUTRITION LA English DT Article DE coprophagy; Ggcx; long-chain menaquinones; menaquinone-4; phylloquinone; suspended wire cages; Ubiad1; vitamin K; Vkorc1; Vkorc1l1 ID GAMMA-GLUTAMYL CARBOXYLASE; MENAQUINONE-4 VITAMIN-K-2; PHYLLOQUINONE VITAMIN-K-1; GLUCOSE-METABOLISM; RATS; DEFICIENT; CONVERSION; BACTERIA; AGE; REQUIREMENTS AB Background: There has been limited characterization of biological variables that impact vitamin K metabolism. This gap in knowledge can limit the translation of data obtained from preclinical animal studies to future human studies. Objective: The purpose of this study was to determine the effects of diet, sex, and housing on serum, tissue, and fecal vitamin K concentrations and gene expression in C57BL6 mice during dietary vitamin K manipulation. Methods: C57BL6 4-mo-old male and female mice were randomly assigned to conventional or suspended-wire cages and fed control [1400 +/- 80 mu g phylloquinone (PK)/kg] or deficient (31 +/- 0.45 mu g PK/kg) diets for 28 d in a factorial design. PK and menaquinone (MK) 4 plasma and tissue concentrations were measured by HPLC. Long-chain MKs were measured in all matrices by LC-atmospheric pressure chemical ionization-mass spectrometry. Gene expression was quantified by reverse transcriptase-polymerase chain reaction in the liver, brain, kidney, pancreas, and adipose tissue. Results: Male and female mice responded differently to dietary manipulation in a tissue-dependent manner. In mice fed the control diet, females had similar to 3-fold more MK4 in the brain and mesenteric adipose tissue than did males and 100% greater PK concentrations in the liver, kidney, and mesenteric adipose tissue than did males. In mice fed the deficient diet, kidney MK4 concentrations were similar to 4-fold greater in females than in males, and there were no differences in other tissues. Males and females differed in the expression of vitamin K expoxide reductase complex 1 (Vkorc1) in mesenteric adipose tissue and the pancreas and ubiA domain-containing protein 1 (Ubiad1) in the kidney and brain. There was no effect of housing on serum, tissue, or fecal concentrations of any vitamin K form. Conclusions: Vitamin K concentrations and expression of key metabolic enzymes differ between male and female mice and in response to the dietary PK concentration. Identifying factors that may impact study design and outcomes of interest is critical to optimize study parameters examining vitamin K metabolism in animal models. C1 [Harshman, Stephanie G.; Fu, Xueyan; Barger, Kathryn; Lamon-Fava, Stefania; Greenberg, Andrew S.; Smith, Donald; Shen, Xiaohua; Booth, Sarah L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Karl, J. Philip] US Army Res Inst Environm Med, Natick, MA USA. [Kuliopulos, Athan] Tufts Univ, Sackler Sch Biomed Sci, Boston, MA 02111 USA. RP Booth, SL (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM sarah.booth@tufts.edu FU USDA Agricultural Research Service [58-1950-7-707]; NIH/NIDDK [T32 DK062032] FX Supported by the USDA Agricultural Research Service under Cooperative Agreement 58-1950-7-707 and NIH/NIDDK grant T32 DK062032 (to SGH). NR 51 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD AUG PY 2016 VL 146 IS 8 BP 1521 EP 1527 DI 10.3945/jn.116.233130 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DZ8OT UT WOS:000386130900008 PM 27385762 ER PT J AU Geary, TW Burns, GW Moraes, JGN Moss, JI Denicol, AC Dobbs, KB Ortega, MS Hansen, PJ Wehrman, ME Neibergs, H O'Neil, E Behura, S Spencer, TE AF Geary, Thomas W. Burns, Gregory W. Moraes, Joao G. N. Moss, James I. Denicol, Anna C. Dobbs, Kyle B. Ortega, M. Sofia Hansen, Peter J. Wehrman, Michael E. Neibergs, Holly O'Neil, Eleanore Behura, Susanta Spencer, Thomas E. TI Identification of Beef Heifers with Superior Uterine Capacity for Pregnancy SO BIOLOGY OF REPRODUCTION LA English DT Article DE endometrium; pregnancy; ruminants (cows sheep, llama, camel); uterus ID HUMAN CHORIONIC-GONADOTROPIN; EARLY EMBRYONIC-DEVELOPMENT; MATERNAL IMMUNE-SYSTEM; LACTATING DAIRY-COWS; ARTIFICIAL-INSEMINATION; ESTROUS-CYCLE; BOVINE ENDOMETRIUM; PROGESTERONE SUPPLEMENTATION; REPRODUCTIVE-PERFORMANCE; TROPHOBLAST PROTEIN-1 AB Infertility and subfertility represent major problems in domestic animals and humans, and the majority of embryonic loss occurs during the first month of gestation that involves pregnancy recognition and conceptus implantation. The critical genes and physiological pathways in the endometrium that mediate pregnancy establishment and success are not well understood. In study one, predominantly Angus heifers were classified based on fertility using serial embryo transfer to select animals with intrinsic differences in pregnancy loss. In each of the four rounds, a single in vitro-produced, high-quality embryo was transferred into heifers on Day 7 postestrus and pregnancy was determined on Days 28 and 42 by ultrasound and then terminated. Heifers were classified based on pregnancy success as high fertile (HF), subfertile (SF), or infertile (IF). In study two, fertility-classified heifers were resynchronized and bred with semen from a single high-fertility bull. Blood samples were collected every other day from Days 0 to 36 postmating. Pregnancy rate was determined on Day 28 by ultrasound and was higher in HF (70.4%) than in heifers with low fertility (36.8%; SF and IF). Progesterone concentrations in serum during the first 20 days postestrus were not different in nonpregnant heifers and also not different in pregnant heifers among fertility groups. In study three, a single in vivo-produced embryo was transferred into fertility-classified heifers on Day 7 postestrus. The uteri were flushed on Day 14 to recover embryos, and endometrial biopsies were obtained from the ipsilateral uterine horn. Embryo recovery rate and conceptus length and area were not different among the heifer groups. RNA was sequenced from the Day 14 endometrial biopsies of pregnant HF, SF, and IF heifers (n - 5 per group) and analyzed by edgeR-robust analysis. There were 26 differentially expressed genes (DEGs) in the HF compared to SF endometrium, 12 DEGs for SF compared to IF endometrium, and three DEGs between the HF and IF endometrium. Several of the DEG-encoded proteins are involved in immune responses and are expressed in B cells. Results indicate that preimplantation conceptus survival and growth to Day 14 is not compromised in SF and IF heifers. Thus, the observed difference in capacity for pregnancy success in these fertility-classified heifers is manifest between Days 14 and 28 when pregnancy recognition signaling and conceptus elongation and implantation must occur for the establishment of pregnancy. C1 [Geary, Thomas W.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Burns, Gregory W.; Moraes, Joao G. N.; O'Neil, Eleanore; Behura, Susanta; Spencer, Thomas E.] Univ Missouri, Div Anim Sci, 158 ASRC,920 East Campus Dr, Columbia, MO 65211 USA. [Moss, James I.; Denicol, Anna C.; Dobbs, Kyle B.; Ortega, M. Sofia; Hansen, Peter J.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Wehrman, Michael E.] Rocky Mt Reprod Serv, Billings, MT USA. [Neibergs, Holly] Washington State Univ, Dept Anim Sci, Pullman, WA 99164 USA. RP Geary, TW (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA.; Spencer, TE (reprint author), Univ Missouri, Div Anim Sci, 158 ASRC,920 East Campus Dr, Columbia, MO 65211 USA. EM tom.geary@ars.usda.gov; spencerte@missouri.edu FU Eunice Kennedy Shriver National Institute of Child Health and Human Development [1 R01 HD072898] FX This research was supported by Grant 1 R01 HD072898 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development. NR 72 TC 1 Z9 1 U1 3 U2 3 PU SOC STUDY REPRODUCTION PI MADISON PA 1691 MONROE ST,SUITE # 3, MADISON, WI 53711-2021 USA SN 0006-3363 EI 1529-7268 J9 BIOL REPROD JI Biol. Reprod. PD AUG 1 PY 2016 VL 95 IS 2 AR 47 DI 10.1095/biolreprod.116.141390 PG 12 WC Reproductive Biology SC Reproductive Biology GA DZ6AB UT WOS:000385942800013 PM 27417907 ER PT J AU Yu, LJ Zhong, SY Heilman, WE Bian, XD AF Yu, Lejiang Zhong, Shiyuan Heilman, Warren E. Bian, Xindi TI The effect of two types of El Nino on the southerly low-level jets in North America SO EARTH AND SPACE SCIENCE LA English DT Article ID CENTRAL UNITED-STATES; GREAT-PLAINS; HEMISPHERE WINTER; BOUNDARY-LAYER; CLIMATOLOGY; PRECIPITATION; MODEL; SIMULATIONS; CIRCULATION; IMPACTS AB Low-level jets (LLJs) are frequent weather phenomena in many regions of North America and have profound impacts on precipitation and wind energy. We used a 31 year (1979-2010) three-hourly reanalysis data set to examine the teleconnection between southerly LLJ activity in North America and the two dominant patterns of the equatorial Pacific Ocean sea surface temperature anomalies characterized by El Nino and El Nino Modoki. We show that El Nino and El Nino Modoki exert different effects on the jet activities, and the results vary by region and by season. Overall, El Nino Modoki affects jet activity all year round, but El Nino's influence is limited mostly to the cold season (October-March). El Nino Modoki induces larger changes in jet frequency, but El Nino's influence extends to larger regions. A better understanding of this teleconnection can be used to improve seasonal predictions of precipitation and wind energy resources in regions of North America. C1 [Yu, Lejiang] Polar Res Inst China, Shanghai, Peoples R China. [Zhong, Shiyuan] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. [Zhong, Shiyuan] Michigan State Univ, Ctr Global Climate Change & Earth Observat, E Lansing, MI 48824 USA. [Heilman, Warren E.; Bian, Xindi] USDA Forest Serv, Northern Res Stn, Lansing, MI USA. RP Zhong, SY (reprint author), Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA.; Zhong, SY (reprint author), Michigan State Univ, Ctr Global Climate Change & Earth Observat, E Lansing, MI 48824 USA. EM zhongs@msu.edu NR 62 TC 0 Z9 0 U1 1 U2 1 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2333-5084 J9 EARTH SPACE SCI JI Earth Space Sci. PD AUG PY 2016 VL 3 IS 8 BP 306 EP 317 DI 10.1002/2016EA000164 PG 12 WC Geosciences, Multidisciplinary SC Geology GA DZ3GZ UT WOS:000385734600003 ER PT J AU Zscheischler, J Fatichi, S Wolf, S Blanken, PD Bohrer, G Clark, K Desai, AR Hollinger, D Keenan, T Novick, KA Seneviratne, SI AF Zscheischler, Jakob Fatichi, Simone Wolf, Sebastian Blanken, Peter D. Bohrer, Gil Clark, Kenneth Desai, Ankur R. Hollinger, David Keenan, Trevor Novick, Kimberly A. Seneviratne, Sonia I. TI Short-term favorable weather conditions are an important control of interannual variability in carbon and water fluxes SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article ID ATMOSPHERE CO2 EXCHANGE; SUB-ALPINE FOREST; DECIDUOUS FOREST; ECOSYSTEM PRODUCTIVITY; TERRESTRIAL BIOSPHERE; TEMPORAL VARIABILITY; INVASIVE INSECTS; CLIMATE EXTREMES; EDDY-COVARIANCE; SEVERE DROUGHT AB Ecosystem models often perform poorly in reproducing interannual variability in carbon and water fluxes, resulting in considerable uncertainty when estimating the land-carbon sink. While many aggregated variables (growing season length, seasonal precipitation, or temperature) have been suggested as predictors for interannual variability in carbon fluxes, their explanatory power is limited and uncertainties remain as to their relative contributions. Recent results show that the annual count of hours where evapotranspiration (ET) is larger than its 95th percentile is strongly correlated with the annual variability of ET and gross primary production (GPP) in an ecosystem model. This suggests that the occurrence of favorable conditions has a strong influence on the annual carbon budget. Here we analyzed data from eight forest sites of the AmeriFlux network with at least 7 years of continuous measurements. We show that for ET and the carbon fluxes GPP, ecosystem respiration (RE), and net ecosystem production, counting the "most active hours/days" (i.e., hours/days when the flux exceeds a high percentile) correlates well with the respective annual sums, with correlation coefficients generally larger than 0.8. Phenological transitions have much weaker explanatory power. By exploiting the relationship between most active hours and interannual variability, we classify hours as most active or less active and largely explain interannual variability in ecosystem fluxes, particularly for GPP and RE. Our results suggest that a better understanding and modeling of the occurrence of large values in high-frequency ecosystem fluxes will result in a better understanding of interannual variability of these fluxes. C1 [Zscheischler, Jakob; Seneviratne, Sonia I.] Swiss Fed Inst Technol, Inst Atmospher & Climate Sci, Zurich, Switzerland. [Fatichi, Simone] Swiss Fed Inst Technol, Inst Environm Engn, Zurich, Switzerland. [Wolf, Sebastian] Swiss Fed Inst Technol, Inst Agr Sci, Zurich, Switzerland. [Blanken, Peter D.] Univ Colorado Boulder, Dept Geog, Boulder, CO USA. [Bohrer, Gil] Ohio State Univ, Dept Civil Environm & Geodet Engn, Columbus, OH 43210 USA. [Clark, Kenneth] US Forest Serv, USDA, Northern Res Stn, New Lisbon, NJ USA. [Desai, Ankur R.] Univ Wisconsin Madison, Dept Atmospher & Ocean Sci, Madison, WI USA. [Hollinger, David] US Forest Serv, USDA, Northern Res Stn, Durham, NH USA. [Keenan, Trevor] Lawrence Berkeley Natl Lab, Berkeley, CA USA. [Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN USA. RP Zscheischler, J (reprint author), Swiss Fed Inst Technol, Inst Atmospher & Climate Sci, Zurich, Switzerland. EM jakob.zscheischler@env.ethz.ch RI Keenan, Trevor/B-2744-2010; Wolf, Sebastian/B-4580-2010; OI Keenan, Trevor/0000-0002-3347-0258; Wolf, Sebastian/0000-0001-7717-6993; Fatichi, Simone/0000-0003-1361-6659; Zscheischler, Jakob/0000-0001-6045-1629 FU U.S. Department of Energy, Office of Science, through the AmeriFlux Management Project (AMP) at Lawrence Berkeley National Laboratory [7094866]; Stavros Niarchos Foundation; ETH Zurich Foundation [ETH-29 14-2]; European Commission [300083] FX The AmeriFlux sites US-MMS and US-NR1 are currently supported by the U.S. Department of Energy, Office of Science, through the AmeriFlux Management Project (AMP) at Lawrence Berkeley National Laboratory under award 7094866. We thank Andrew Richardson for providing the data of Bartlett Experimental Forest. S.F. thanks the Stavros Niarchos Foundation and the ETH Zurich Foundation (grant ETH-29 14-2) for their support. S.W. was supported by the European Commission with a Marie Curie International Outgoing Fellowship (grant 300083). All used data are available from the AmeriFlux network (www.ameriflux.lbl.gov). NR 45 TC 0 Z9 0 U1 11 U2 11 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD AUG PY 2016 VL 121 IS 8 BP 2186 EP 2198 DI 10.1002/2016JG003503 PG 13 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DZ2ZP UT WOS:000385712400013 PM 27774367 ER PT J AU Li, MHH Lucas, PM Kingery, K Bosworth, BG AF Li, Menghe H. Lucas, Penelope M. Kingery, Kyle Bosworth, Brian G. TI Efficacy of Crystalline Lysine in Alternative Diets for Pond-raised Hybrid Catfish, female Ictalurus punctatus x male Ictalurus furcatus SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID FINGERLING CHANNEL CATFISH; PROTEIN-BOUND LYSINE; CORN GLUTEN FEED; SOYBEAN-MEAL; COTTONSEED MEAL; GROWTH; REQUIREMENT AB A study was conducted to examine the efficacy of crystalline lysine in alternative diets for pond-raised hybrid catfish, female Ictalurus punctatus x male Ictalurus furcatus. Two 28% protein alternative diets supplemented with l-lysine HCl at the required level based on 62% (previously published value) or 100% lysine availability were compared with a traditional 28% protein control diet. Hybrid catfish fingerlings (mean initial weight = 43 g/fish) were stocked into 15 earthen ponds (0.04 ha) at a density of 14,826 fish/ha with five ponds per treatment. Fish were fed once daily to apparent satiation for a 173-d growing season. There were no significant differences in total diet fed, net yield, weight gain, and survival among dietary treatments. There were also no significant differences in carcass yield, fillet yield, and fillet proximate composition and fillet lysine concentration among treatments. Fish fed the traditional control diet had slightly, but significantly, lower feed conversion ratio than fish fed alternative diets, which is likely related to higher dietary fiber levels in the alternative diets. Results from this study show that crystalline lysine can be considered 100% available when used to supplement lysine-deficient diets for pond-raised hybrid catfish. C1 [Li, Menghe H.; Lucas, Penelope M.; Kingery, Kyle] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. [Bosworth, Brian G.] ARS, USDA, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. RP Li, MHH (reprint author), Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. EM mli@drec.msstate.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [MIS-371590] FX We thank Billy Rutland and staff at the National Warmwater Aquaculture Center pond facility for assistance in routine management of the study, and Sandra Phillips and Will Rutland for their assistance with feeding and proximate analysis of diet and tissue samples. This manuscript is approved for publication as Journal Article No. J-12704 of the Mississippi Agricultural and Forestry Experiment Station, Mississippi State University. This material is based upon work supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Project No. MIS-371590. Reference to trade names does not imply endorsement by the U.S. Government. NR 17 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD AUG PY 2016 VL 47 IS 4 BP 519 EP 525 DI 10.1111/jwas.12285 PG 7 WC Fisheries SC Fisheries GA DY8QV UT WOS:000385395800002 ER PT J AU Pfalzer, AC Kamanu, FK Parnell, LD Tai, AK Liu, ZH Mason, JB Crott, JW AF Pfalzer, Anna C. Kamanu, Frederick K. Parnell, Laurence D. Tai, Albert K. Liu, Zhenhua Mason, Joel B. Crott, Jimmy W. TI Interactions between the colonic transcriptome, metabolome, and microbiome in mouse models of obesity-induced intestinal cancer SO PHYSIOLOGICAL GENOMICS LA English DT Article DE obesity; Akt; transcriptome; metabolome; microbiome ID SIGNALING PATHWAY; NETWORK ANALYSIS; INFLAMMATION; AKT; PROLIFERATION; EXPRESSION; RESISTANCE; PI3K/AKT; GROWTH; GENE AB Obesity is a significant risk factor for colorectal cancer (CRC); however, the relative contribution of high-fat (HF) consumption and excess adiposity remains unclear. It is becoming apparent that obesity perturbs both the intestinal microbiome and metabolome, and each has the potential to induce protumorigenic changes in the epithelial transcriptome. The physiological consequences and the degree to which these different biologic systems interact remain poorly defined. To understand the mechanisms by which obesity drives colonic tumorigenesis, we profiled the colonic epithelial transcriptome of HF-fed and genetically obese (DbDb) mice with a genetic predisposition to intestinal tumorigenesis (Apc(1638N)); 266 and 584 genes were differentially expressed in the colonic mucosa of HF and DbDb mice, respectively. These genes mapped to pathways involved in immune function, and cellular proliferation and cancer. Furthermore, Akt was central within the networks of interacting genes identified in both gene sets. Regression analyses of coexpressed genes with the abundance of bacterial taxa identified three taxa, previously correlated with tumor burden, to be significantly correlated with a gene module enriched for Akt-related genes. Similarly, regression of coexpressed genes with metabolites found that adenosine, which was negatively associated with inflammatory markers and tumor burden, was also correlated with a gene module enriched with Akt regulators. Our findings provide evidence that HF consumption and excess adiposity result in changes in the colonic transcriptome that, although distinct, both appear to converge on Akt signaling. Such changes could be mediated by alterations in the colonic microbiome and metabolome. C1 [Pfalzer, Anna C.; Parnell, Laurence D.; Liu, Zhenhua; Mason, Joel B.; Crott, Jimmy W.] Tufts Univ, USDA Jean Mayer Human Nutr Res Ctr Aging, Canc Cluster, Boston, MA 02111 USA. [Pfalzer, Anna C.; Mason, Joel B.; Crott, Jimmy W.] Tufts Univ, USDA Jean Mayer Human Nutr Res Ctr Aging, Vitamins & Carcinogenesis Lab, Boston, MA 02111 USA. [Pfalzer, Anna C.; Mason, Joel B.; Crott, Jimmy W.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Kamanu, Frederick K.] Tufts Univ, USDA Jean Mayer Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Parnell, Laurence D.] Tufts Univ, ARS, USDA, Jean Mayer Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tai, Albert K.] Tufts Univ, Sch Med, Genom Core, Boston, MA 02111 USA. [Liu, Zhenhua] Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Amherst, MA 01003 USA. RP Crott, JW (reprint author), Tufts Univ, USDA, HNRCA, 711 Washington St, Boston, MA 02111 USA. EM Jimmy.crott@tufts.edu FU Agricultural Research Service of the United States Department of Agriculture [58-1950-0-014, 58-1950-4-003]; Tufts Collaborates Seed Initiative from Office of the Vice Provost; HNRCA Directors Student Innovation Fund FX This study was funded in part by the Agricultural Research Service of the United States Department of Agriculture, Cooperative Agreements #58-1950-0-014 and #58-1950-4-003. Additional support for this work was provided by the Tufts Collaborates Seed Initiative from the Office of the Vice Provost and Drs. Peter and Joan Cohn through the HNRCA Directors Student Innovation Fund. Opinions and ideas expressed in this publication are not necessarily those of the U.S. Department of Agriculture. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Department of Agriculture. NR 44 TC 0 Z9 0 U1 5 U2 5 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 1094-8341 EI 1531-2267 J9 PHYSIOL GENOMICS JI Physiol. Genomics PD AUG 1 PY 2016 VL 48 IS 8 BP 545 EP 553 DI 10.1152/physiolgenomics.00034.2016 PG 9 WC Cell Biology; Genetics & Heredity; Physiology SC Cell Biology; Genetics & Heredity; Physiology GA DY7EP UT WOS:000385292700001 PM 27317588 ER PT J AU Aguilar-Rivera, C Hume, ME Klotz-Ceberio, BF AF Aguilar-Rivera, C. Hume, M. E. Klotz-Ceberio, B. F. TI INHIBITORY EFFECT OF SUPERNATANTS FROM A COMPETITIVE EXCLUSION CULTURE OVER GROWTH OF SOME INTESTINAL PATHOGENS SO REVISTA MEXICANA DE INGENIERIA QUIMICA LA Spanish DT Article DE competitive exclusion culture; bacteriocins; kinetic parameters; intestinal pathogens; inhibitory effect ID LACTIC-ACID BACTERIA; MICROBIAL ECOSYSTEMS THERAPEUTICS; PROBIOTICS; FOOD; POULTRY; STRAINS; MODELS; FLORA; SPP. AB Competitive exclusion cultures (CEC), which use whole bacterial communities derived directly from the gastrointestinal tract, have been effective to control of pathogens in animals, but its application in humans has not been widely studied. In this work, the inhibitory effect of supernatants obtained from a CEC developed from fecal samples of healthy individuals, was quantified over the growth of intestinal pathogens of public health significance. Baranyi Model was adjusted (R-2 >0.947) to growth curves of Shigella sonnei-(SF1), E. coli EPEC-(EC6) y Salmonella Enteritidis-(SE3) treated with supernatants, in order to estimate kinetic parameters. Both, growth rate and pathogen concentrations were significantly reduced by the action of supernatants in 89.89% and 86.80%, respectively, for the most sensitive bacteria (SF1) and 23.21% and 36.86%, for the most resistant (SE3). A growth delay of more than 14 hours was presented by SF1 and EC6 with the treatments. Thermal and enzymatic (proteinase K and trypsin) sensitivity of supernatants indicate that antagonism was mediated by bacteriocin-like substances. The CEC developed, have biotechnological potential for the production of antimicrobial substances of interest in the food and pharmaceutical industries. C1 [Aguilar-Rivera, C.] Univ La Sabana, Fac Ingn, Doctorado Biociencias, Km 7, Autopista Norte Bogota, Chia, Colombia. [Hume, M. E.] ARS, USDA, SPARC, College Stn, TX USA. [Klotz-Ceberio, B. F.] Alpina Corp SA, Inst Alpina Invest, Km 3 Via Briceno Sopo, Sopo, Cundinamarca, Colombia. RP Klotz-Ceberio, BF (reprint author), Alpina Corp SA, Inst Alpina Invest, Km 3 Via Briceno Sopo, Sopo, Cundinamarca, Colombia. EM bernadette.klotz@alpina.com NR 47 TC 0 Z9 0 U1 1 U2 1 PU UNIV AUTONOMA METROPOLITANA-IZTAPALAPA PI MEXICO PA C/O DR JAIME VERNON-CARTER, SAN RAFAEL ATLIXCO NO 186, COL VICENTINA, DELEGACION IZTAPALAPA, MEXICO, 09340, MEXICO SN 1665-2738 J9 REV MEX ING QUIM JI Rev. Mex. Ing. Quim. PD AUG PY 2016 VL 15 IS 2 BP 379 EP 389 PG 11 WC Chemistry, Applied; Engineering, Chemical SC Chemistry; Engineering GA DY9QA UT WOS:000385468600007 ER PT J AU Berdanier, AB Miniat, CF Clark, JS AF Berdanier, Aaron B. Miniat, Chelcy F. Clark, James S. TI Predictive models for radial sap flux variation in coniferous, diffuse-porous and ring-porous temperate trees SO TREE PHYSIOLOGY LA English DT Article DE hierarchical Bayes; plant hydraulics; radial profile; water use; wood anatomy ID HEAT-PULSE VELOCITY; PLANT WATER-USE; THERMAL DISSIPATION; FLOW MEASUREMENTS; OAK TREES; DENSITY; SAPWOOD; TRUNKS; FOREST; PATTERNS AB Accurately scaling sap flux observations to tree or stand levels requires accounting for variation in sap flux between wood types and by depth into the tree. However, existing models for radial variation in axial sap flux are rarely used because they are difficult to implement. there is uncertainty about their predictive ability and calibration measurements are often unavailable. Here we compare different models with a diverse sap flux data set to test the hypotheses that radial profiles differ by wood type and tree size. We show that radial variation in sap flux is dependent on wood type but independent of tree size for a range of temperate trees. The best-fitting model predicted out-of-sample sap flux observations and independent estimates of sapwood area with small errors, suggesting robustness in the new settings. We develop a method for predicting whole-tree water use with this model and include computer code for simple implementation in other studies. C1 [Berdanier, Aaron B.] Duke Univ, Univ Program Ecol, Durham, NC 27708 USA. [Berdanier, Aaron B.; Clark, James S.] Duke Univ, Levine Sci Res Ctr A311, Nicholas Sch Environm, Durham, NC 27708 USA. [Miniat, Chelcy F.] US Forest Serv, Coweeta Hydrol Lab, USDA, Southern Res Stn, Otto, NC 28763 USA. [Clark, James S.] Duke Univ, Dept Stat Sci, Durham, NC 27708 USA. RP Berdanier, AB (reprint author), Duke Univ, Univ Program Ecol, Durham, NC 27708 USA.; Berdanier, AB (reprint author), Duke Univ, Levine Sci Res Ctr A311, Nicholas Sch Environm, Durham, NC 27708 USA. EM aaron.berdanier@gmail.com FU Coweeta Hydrologic Laboratory, US Department of Agriculture Forest Service; Southern Research Station; Coweeta Long Term Ecological Research project - National Science Foundation [DEB-0823293] FX This research was supported by Coweeta Hydrologic Laboratory, US Department of Agriculture Forest Service and Southern Research Station, and the Coweeta Long Term Ecological Research project funded by National Science Foundation grant DEB-0823293. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the US Department of Agriculture of any product or service. NR 58 TC 1 Z9 1 U1 11 U2 11 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD AUG PY 2016 VL 36 IS 8 BP 932 EP 941 DI 10.1093/treephys/tpw027 PG 10 WC Forestry SC Forestry GA DY7EG UT WOS:000385291800002 PM 27126230 ER PT J AU Carlo, NJ Renninger, HJ Clark, KL Schafer, KVR AF Carlo, Nicholas J. Renninger, Heidi J. Clark, Kenneth L. Schafer, Karina V. R. TI Impacts of prescribed fire on Pinus rigida Mill. in upland forests of the Atlantic Coastal Plain SO TREE PHYSIOLOGY LA English DT Article DE forest disturbance; forest fire; gas exchange; sap-flux; water use ID MICROBIAL COMMUNITY STRUCTURE; SAP FLOW MEASUREMENTS; NEW-JERSEY PINELANDS; WATER-USE EFFICIENCY; PONDEROSA PINE; CARBON SEQUESTRATION; THERMAL DISSIPATION; NUTRIENT DYNAMICS; TALLGRASS PRAIRIE; INVASIVE INSECTS AB A comparative analysis of the impacts of prescribed fire on three upland forest stands in the Northeastern Atlantic Plain, NJ, USA, was conducted. Effects of prescribed fire on water use and gas exchange of overstory pines were estimated via sap-flux rates and photosynthetic measurements on Pinus rigida Mill. Each study site had two sap-flux plots, one experiencing prescribed fire and one control (unburned) plot for comparison before and after the fire. We found that photosynthetic capacity in terms of Rubisco-limited carboxylation rate and intrinsic water-use efficiency was unaffected, while light compensation point and dark respiration rate were significantly lower in the burned vs control plots post-fire. Furthermore, quantum yield in pines in the pine dominated stands was less affected than pines in the mixed oak/pine stand, as there was an increase in quantum yield in the oak/pine stand post-fire compared with the control (unburned) plot. We attribute this to an effect of forest type but not fire per se. Average daily sap-flux rates of the pine trees increased compared with control (unburned) plots in pine-dominated stands and decreased in the oak/pine stand compared with control (unburned) plots, potentially due to differences in fuel consumption and pre-fire sap-flux rates. Finally, when reference canopy stomatal conductance was analyzed, pines in the pine-dominated stands were more sensitive to changes in vapor pressure deficit (VPD), while stomatal responses of pines in the oak/pine stand were less affected by VPD. Therefore, prescribed fire affects physiological functioning and water use of pines, but the effects may be modulated by forest stand type and fuel consumption pattern, which suggests that these factors may need to be taken into account for forest management in fire-dominated systems. C1 [Carlo, Nicholas J.; Schafer, Karina V. R.] Rutgers State Univ, Dept Earth & Environm Sci, 101 Warren St, Newark, NJ 07102 USA. [Renninger, Heidi J.; Schafer, Karina V. R.] Rutgers State Univ, Dept Biol Sci, 195 Univ Ave, Newark, NJ 07012 USA. [Renninger, Heidi J.] Mississippi State Univ, Dept Forestry, Thompson Hall,Box 9681, Mississippi State, MS 39762 USA. [Clark, Kenneth L.] US Forest Serv, Silas Little Expt Forest, USDA, 501 Four Mile Rd, New Lisbon, NJ 08064 USA. RP Schafer, KVR (reprint author), Rutgers State Univ, Dept Earth & Environm Sci, 101 Warren St, Newark, NJ 07102 USA.; Schafer, KVR (reprint author), Rutgers State Univ, Dept Biol Sci, 195 Univ Ave, Newark, NJ 07012 USA. EM karinavr@andromeda.rutgers.edu FU United States Department of Agriculture [10-JV-11242306-136]; U.S. Department of Energy, Office of Science (Biological and Environmental research), United States Department of Energy [DE-SC0007041] FX This research was supported by United States Department of Agriculture joint venture agreement 10-JV-11242306-136 and by the U.S. Department of Energy, Office of Science (Biological and Environmental research), United States Department of Energy under award number DE-SC0007041 both to K.V.R.S. NR 110 TC 0 Z9 0 U1 8 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD AUG PY 2016 VL 36 IS 8 BP 967 EP 982 DI 10.1093/treephys/tpw044 PG 16 WC Forestry SC Forestry GA DY7EG UT WOS:000385291800005 PM 27259637 ER PT J AU Corcoran, MP Nelson, ME Sacheck, JM Reid, KF Kirn, D Fielding, RA Folta, SC AF Corcoran, Michael P. Nelson, Miriam E. Sacheck, Jennifer M. Reid, Kieran F. Kirn, Dylan Fielding, Roger A. Folta, Sara C. TI Recruitment of Mobility Limited Older Adults Into a Facility-Led Exercise-Nutrition Study: The Effect of Social Involvement SO GERONTOLOGIST LA English DT Article DE Recruiting; Assisted living; Senior housing; Enrollment; Participation ID LOWER-EXTREMITY FUNCTION; INFORMED-CONSENT; STRATEGIES; STRENGTH; HEALTH; INTERVENTIONS; PERFORMANCE; PREDICTORS; DISABILITY; RETENTION AB Purpose of the Study: Older adults are among the most challenging population groups to enroll into health-related research. This article describes two methods used by investigators to recruit mobility limited older adults residing at assisted living or senior housing (SH) facilities into a facility-led exercise-nutrition research study. Design and Methods: Sedentary older adults were recruited from 42 different assisted living facilities (ALFs) or SH communities. Two different recruitment approaches were used: At 22 sites, investigators conducted heavily advertised informational sessions to recruit participants (Info only). At 20 locations, these sessions were preceded by attendance of a study team member at various activities offered by the facility over the preceding 2 weeks (activity attendance). Population reach, enrollment, personnel cost, and time required to recruit at least five participants at each facility was measured. Reasons for declining participation and withdrawal rate were also measured. Results: Sixty percent more residents elected to be screened for eligibility when study personnel attended an activity offered by the facility. Activity attendance resulted in significantly less time, costs, and participant withdrawals compared with facilities with no activity attendance. Implications: Study team member attendance at activities offered by senior living facilities reduces cost and duration of recruitment and improves study retention. Interventions targeting this demographic are likely to benefit from deliberately building trust and familiarity among the resident population at senior living communities as part of the recruitment process. C1 [Corcoran, Michael P.; Nelson, Miriam E.; Sacheck, Jennifer M.; Fielding, Roger A.; Folta, Sara C.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Corcoran, Michael P.] Merrimack Coll, Dept Hlth Sci, N Andover, MA 01845 USA. [Reid, Kieran F.; Kirn, Dylan; Fielding, Roger A.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. RP Corcoran, MP (reprint author), Merrimack Coll, Dept Hlth Sci, N Andover, MA 01845 USA. EM corcoranm@merrimack.edu NR 29 TC 0 Z9 0 U1 3 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0016-9013 EI 1758-5341 J9 GERONTOLOGIST JI Gerontologist PD AUG PY 2016 VL 56 IS 4 BP 669 EP 676 DI 10.1093/geront/gnv018 PG 8 WC Gerontology SC Geriatrics & Gerontology GA DY0AU UT WOS:000384760000010 PM 26035904 ER PT J AU Smith, CE Fullerton, SM Dookeran, KA Hampel, H Tin, A Maruthur, NM Schisler, JC Henderson, JA Tucker, KL Ordovas, JM AF Smith, Caren E. Fullerton, Stephanie M. Dookeran, Keith A. Hampel, Heather Tin, Adrienne Maruthur, Nisa M. Schisler, Jonathan C. Henderson, Jeffrey A. Tucker, Katherine L. Ordovas, Jose M. TI Using Genetic Technologies To Reduce, Rather Than Widen, Health Disparities SO HEALTH AFFAIRS LA English DT Article ID STAGE RENAL-DISEASE; CAROLINA BREAST-CANCER; RACIAL-DIFFERENCES; UNITED-STATES; SOCIOECONOMIC-STATUS; KIDNEY-DISEASE; POPULATION; VARIANTS; AFRICAN; DIAGNOSIS AB Evidence shows that both biological and nonbiological factors contribute to health disparities. Genetics, in particular, plays a part in how common diseases manifest themselves. Today, unprecedented advances in genetically based diagnoses and treatments provide opportunities for personalized medicine. However, disadvantaged groups may lack access to these advances, and treatments based on research on non-Hispanic whites might not be generalizable to members of minority groups. Unless genetic technologies become universally accessible, existing disparities could be widened. Addressing this issue will require integrated strategies, including expanding genetic research, improving genetic literacy, and enhancing access to genetic technologies among minority populations in a way that avoids harms such as stigmatization. C1 [Smith, Caren E.; Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fullerton, Stephanie M.] Univ Washington, Dept Bioeth & Humanities, Seattle, WA 98195 USA. [Dookeran, Keith A.] Univ Illinois, Sch Publ Hlth, Div Epidemiol & Biostat, Chicago, IL USA. [Dookeran, Keith A.] Canc Fdn Minor & Underserved Populat, Chicago, IL USA. [Hampel, Heather] Ohio State Univ, Div Human Genet, Ctr Comprehens Canc, Columbus, OH 43210 USA. [Tin, Adrienne; Maruthur, Nisa M.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Maruthur, Nisa M.] Johns Hopkins Univ, Sch Med, Div Gen Internal Med, Baltimore, MD 21218 USA. [Maruthur, Nisa M.] Johns Hopkins Univ, Welch Ctr Prevent Epidemiol & Clin Res, Baltimore, MD 21218 USA. [Schisler, Jonathan C.] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC USA. [Henderson, Jeffrey A.] Black Hills Ctr Amer Indian Hlth, Rapid City, SD USA. [Tucker, Katherine L.] Univ Massachusetts, Clin Lab & Nutr Sci, Lowell, MA USA. RP Smith, CE (reprint author), Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM caren.smith@tufts.edu OI Dookeran, Keith/0000-0003-3695-9873 FU Centers for Population Health and Health Disparities grant from the National Cancer Institute; Centers for Population Health and Health Disparities grant from National Heart, Lung, and Blood Institute (NHLBI); Understanding and preventing breast cancer disparities in Latinas [P50CA148143-01]; Lung Cancer Disparities Center: jointly addressing race and socioeconomic status [P50CA148596-01]; Johns Hopkins Center to Eliminate Cardiovascular Health Disparities [P50HL105187-01]; Boston Puerto Rican Health Study-CVD risk factors [P50HL105185-01]; Reducing cervical cancer in Appalachia [P50CA105632-06]; Rush Center for Urban Health Equity [P50HL105189-01]; Family and neighborhood interventions to reduce heart disease risk in East Los Angeles [P50HL105188-01]; University of Illinois at Chicago Center for Population Health and Health Disparities [P50CA106743-06]; Center for Reduced CVD Disparities: genes, clinics, and communities [P50HL105184-01]; Center for Native Population Health Disparities [P50CA148110-01]; NHLBI [K08 HL112845]; National Research Service Award [T32DK007732] FX The authors were supported by the following Centers for Population Health and Health Disparities grants from the National Cancer Institute and the National Heart, Lung, and Blood Institute (NHLBI). Beti Thompson, principal investigator (PI), Understanding and preventing breast cancer disparities in Latinas (Grant No. P50CA148143-01); David R. Williams, PI, Lung Cancer Disparities Center: jointly addressing race and socioeconomic status (Grant No. P50CA148596-01); Lisa A. Cooper, PI, Johns Hopkins Center to Eliminate Cardiovascular Health Disparities (Grant No. P50HL105187-01); Katherine L. Tucker, PI, Boston Puerto Rican Health Study-CVD risk factors (Grant No. P50HL105185-01); Electra D. Paskett, PI, Reducing cervical cancer in Appalachia (Grant No. P50CA105632-06); Lynda H. Powell, PI, Rush Center for Urban Health Equity (Grant No. P50HL105189-01); Alexander N. Ortega, PI, Family and neighborhood interventions to reduce heart disease risk in East Los Angeles (Grant No. P50HL105188-01); Richard Warnecke, PI, University of Illinois at Chicago Center for Population Health and Health Disparities (Grant No. P50CA106743-06); Alice S. Ammerman and Cam Patterson, PIs, Center for Reduced CVD Disparities: genes, clinics, and communities (Grant No. P50HL105184-01); and Dedra S. Buchwald, PI, Center for Native Population Health Disparities (Grant No. P50CA148110-01). Individual authors were also supported by the following awards and organizations: Caren Smith by the NHLBI (Grant No. K08 HL112845); Keith Dookeran by the Komen Foundation and the Cancer Foundation for Minority and Underserved Populations; and Adrienne Tin by the Renal Disease Epidemiology Training Program at the National Institute of Diabetes and Digestive and Kidney Diseases (National Research Service Award No. T32DK007732). NR 47 TC 0 Z9 0 U1 3 U2 3 PU PROJECT HOPE PI BETHESDA PA 7500 OLD GEORGETOWN RD, STE 600, BETHESDA, MD 20814-6133 USA SN 0278-2715 J9 HEALTH AFFAIR JI Health Aff. PD AUG PY 2016 VL 35 IS 8 BP 1367 EP 1373 DI 10.1377/hlthaff.2015.1476 PG 7 WC Health Care Sciences & Services; Health Policy & Services SC Health Care Sciences & Services GA DY0QO UT WOS:000384801500005 PM 27503959 ER PT J AU Bradley, LK Bauske, EM Bewick, TA Clark, JR Durham, RE Langellotto, G Meyer, MH Pooler, M Dorn, S AF Bradley, Lucy K. Bauske, Ellen M. Bewick, Thomas A. Clark, John R. Durham, Richard. E. Langellotto, Gail Meyer, Mary H. Pooler, Margaret Dorn, Sheri TI Developing a National Strategic Plan for Consumer Horticulture SO HORTTECHNOLOGY LA English DT Article DE gardening; funding; stakeholder ID CHALLENGES; IMPACTS AB Consumer horticulture encompasses a wide array of activities that are practiced by and of interest to the gardening public, garden-focused nongovernmental organizations, and gardening-related industries. In a previous publication, we described the current lack of funding for research, extension, and education in consumer horticulture and outlined the need for a strategic plan. Here, we describe our process and progress in crafting a plan to guide university efforts in consumer horticulture, and to unite these efforts with stakeholders' goals. In 2015, a steering committee developed a first draft of a plan, including a mission statement, aspirational vision, core values, goals, and objectives. This draft was subsequently presented to and vetted by stakeholders at the 2015 American Society for Horticultural Science Consumer Horticulture and Master Gardeners (CHMG) working group workshop, a 2015 Extension Master Gardener Coordinators' webinar, and a 2015 meeting in Washington, DC. Feedback received from these events is being used to refine and focus plan goals and objectives. The most recent working draft of the plan can be found on the website, where stakeholders and other interested parties can register to receive updates and to provide input into the process. C1 [Bradley, Lucy K.] North Carolina State Univ, Dept Hort Sci, Campus Box 7609,Kilgore Hall 128,2721 Founders Dr, Raleigh, NC 27695 USA. [Bauske, Ellen M.] Univ Georgia, Ctr Urban Agr, Expt Stn 1109, Griffin, GA 30223 USA. [Bewick, Thomas A.] Natl Inst Food & Agr, Inst Food Prod & Sustainabil, USDA, 1400 Independence Ave SW,Mailstop 2240, Washington, DC 20250 USA. [Clark, John R.] Univ Arkansas, Dept Hort, 316 Plant Sci Bldg, Fayetteville, AR 72701 USA. [Durham, Richard. E.] Univ Kentucky, Dept Hort, N-308G Agr Sci North, Lexington, KY 40546 USA. [Langellotto, Gail] Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97331 USA. [Meyer, Mary H.] Univ Minnesota, 424 Alderman Hall,1970 Folwell Ave, St Paul, MN 55108 USA. [Pooler, Margaret] ARS, US Natl Arboretum, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Dorn, Sheri] Univ Georgia, Dept Hort, Expt Stn 1109, Griffin, GA 30223 USA. RP Bradley, LK (reprint author), North Carolina State Univ, Dept Hort Sci, Campus Box 7609,Kilgore Hall 128,2721 Founders Dr, Raleigh, NC 27695 USA. EM lucy_bradley@ncsu.edu FU Consumer Horticulture and Master Gardeners Working Group; Facilitating the Development of a National Strategic Plan for Consumer Horticulture [USDA-NIFA-2015-38831-24022] FX This paper was part of the workshop "Moving the National Strategic Plan for Consumer Horticulture, Research, Education, and Extension Forward: Creative Destruction and Rebuilding'' held 6 Aug. 2015 at the ASHS Conference, New Orleans, LA, and sponsored by the Consumer Horticulture and Master Gardeners Working Group.; Funding for this research was provided by the Facilitating the Development of a National Strategic Plan for Consumer Horticulture (USDA-NIFA-2015-38831-24022). NR 13 TC 0 Z9 0 U1 2 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2016 VL 26 IS 4 BP 372 EP 378 PG 7 WC Horticulture SC Agriculture GA DY3IH UT WOS:000384984200001 ER PT J AU Goenaga, R Jenkins, D AF Goenaga, Ricardo Jenkins, David TI Yield and Fruit Quality Traits of Atemoya Hybrids Grown in Puerto Rico SO HORTTECHNOLOGY LA English DT Article DE tropical horticulture; tropical fruits; soluble solids oncentration; Annona squamosa x A. cherimola ID BEETLES COLEOPTERA; NITIDULIDAE AB As consumers seek healthy and more diverse food products, the demand for tropical fruits has increased significantly during the last 15 years. There is a lack of formal experimentation to determine the yield performance and fruit quality traits of atemoya (Annona squamosa x A. cherimola) hybrids. Six atemoya hybrids ('Bradley', 'Geffner', 'Priestly', 'Lisa', '47-18', and '75-9') grown on an Oxisol soil were evaluated for 4 years at Isabela, PR. 'Geffner' and 'Lisa' had the highest number of marketable fruit averaging 8542 fruit/ha, and the highest yield of marketable fruit, averaging 1507 kg.ha(-1); they did not differ from each other, but were greater than all other hybrids. Individual weight of marketable fruit was significantly higher in '75-9' and 'Priestly' which averaged 264.8 g. Significantly higher soluble solids concentration values were obtained from fruit of '75-9', 'Bradley', and 'Geffner' which averaged 23.8%; they did not differ from each other, but were greater than all other hybrids. C1 [Goenaga, Ricardo; Jenkins, David] USDA ARS, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. RP Goenaga, R (reprint author), USDA ARS, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. EM ricardo.goenaga@ars.usda.gov NR 19 TC 0 Z9 0 U1 1 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2016 VL 26 IS 4 BP 547 EP 551 PG 5 WC Horticulture SC Agriculture GA DY3IH UT WOS:000384984200023 ER PT J AU Dunlap, CA Saunders, LP Schisler, DA Leathers, TD Naeem, N Cohan, FM Rooney, AP AF Dunlap, Christopher A. Saunders, Lauren P. Schisler, David A. Leathers, Timothy D. Naeem, Naveed Cohan, Frederick M. Rooney, Alejandro P. TI Bacillus nakamurai sp nov., a black-pigment-producing strain SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID ATROPHAEUS; DNA AB Two isolates of a Gram-stain-positive, strictly aerobic, motile, rod-shaped, endospore-forming bacterium were identified during a survey of the Bacillus diversity of the Agriculture Research Service Culture Collection. These strains were originally isolated from soil and have a phenotype of producing a dark pigment on tryptic soy agar. Phylogenetic analysis of the 16S rRNA gene indicated that these strains were related most closely to Bacillus subtilis subsp. inaquosorum (99.7% similarity) and Bacillus axarquiensis (99.7 %). In phenotypic characterization, the novel strains were found to grow between 17 and 50 degrees C and can tolerate up to 9% (w/v) NaCl. Furthermore, the strains grew in media of pH 5.5-10 (optimal growth at pH 7.0-8.0). The predominant cellular fatty acids were anteiso-C-15:0 (34.8 %) and iso-C-15:0 (21.9 %). The cell-wall peptidoglycan contained meso-diaminopimelic acid. A draft genome of both strains was completed. The DNA G+C content was 43.8 mol%. A phylogenomic analysis on the core genome of these two new strains and all members of the Bacillus subtilis group revealed these two strains formed a distinct monophyletic clade with the nearest neighbour Bacillus amyloliquefaciens. DNA-DNA relatedness studies using in silico DNA-DNA hybridizations showed the two strains were conspecific (93.8 %), while values with all other species (< 31.5 %) were well below the species threshold of 70 %. Based on the consensus of phylogenetic and phenotypic analyses, these strains are considered to represent a novel species within the genus Bacillus, for which the name Bacillus nakamurai sp. nov. is proposed, with type strain NRRL B-41091(T) (= CCUG 68786(T)). C1 [Dunlap, Christopher A.; Schisler, David A.; Rooney, Alejandro P.] Natl Ctr Agr Utilizat Res, Crop Bioprotect, 1815 N Univ St, Peoria, IL 61604 USA. [Saunders, Lauren P.; Leathers, Timothy D.] ARS, Renewable Prod Technol Res Units, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL USA. [Naeem, Naveed; Cohan, Frederick M.] Wesleyan Univ, Dept Biol, Middletown, CT 06459 USA. RP Dunlap, CA (reprint author), Natl Ctr Agr Utilizat Res, Crop Bioprotect, 1815 N Univ St, Peoria, IL 61604 USA. EM christopher.dunlap@ars.usda.gov NR 14 TC 0 Z9 0 U1 2 U2 2 PU MICROBIOLOGY SOC PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD AUG PY 2016 VL 66 BP 2987 EP 2991 DI 10.1099/ijsem.0.001135 PN 8 PG 5 WC Microbiology SC Microbiology GA DY2KG UT WOS:000384920900033 PM 27150918 ER PT J AU Cole, NA Radcliff, S DeVries, TJ Rotz, A Ely, DG Cardoso, F AF Cole, N. Andy Radcliff, S. DeVries, T. J. Rotz, A. Ely, D. G. Cardoso, F. TI PRODUCTION, MANAGEMENT, AND ENVIRONMENT SYMPOSIUM: Environmental footprint of livestock production - Greenhouse gas emissions and climate change SO JOURNAL OF ANIMAL SCIENCE LA English DT Editorial Material C1 [Cole, N. Andy] ARS, USDA, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Radcliff, S.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [DeVries, T. J.] Univ Guelph, Dept Anim Biosci, Guelph, ON N1G 2W1, Canada. [Rotz, A.] ARS, USDA, University Pk, PA 16802 USA. [Ely, D. G.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Cardoso, F.] Univ Illinois, Dept Anim Sci, 328 Mumford Hall, Urbana, IL 61801 USA. RP Cole, NA (reprint author), ARS, USDA, Conservat & Prod Res Lab, Bushland, TX 79012 USA. EM nacole@suddenlink.net NR 5 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2016 VL 94 IS 8 BP 3137 EP 3138 DI 10.2527/jas2016-0602 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PK UT WOS:000385005900005 ER PT J AU Richeson, JT Carroll, JA Sanchez, NCB May, ND Hughes, HD Roberts, SL Broadway, PR Sharon, KP Ballou, MA AF Richeson, J. T. Carroll, J. A. Sanchez, N. C. Burdick May, N. D. Hughes, H. D. Roberts, S. L. Broadway, P. R. Sharon, K. P. Ballou, M. A. TI Dexamethasone treatment differentially alters viral shedding and the antibody and acute phase protein response after multivalent respiratory vaccination in beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE antibody; cattle; haptoglobin; immunosuppression; vaccination ID PASTEURELLA-HAEMOLYTICA LEUKOTOXIN; DIARRHEA VIRUS; BOVINE HERPESVIRUS-1; CALVES; GLUCOCORTICOIDS; CATTLE; NEUTROPHILS; STRESS; INFLAMMATION; PERFORMANCE AB Our objective was to examine immunosuppression induced by dexamethasone (DEX) administration in cattle on immunological responses to a multivalent respiratory vaccine containing replicating and nonreplicating agents. Steers (n = 32; 209 +/- 8 kg) seronegative to infectious bovine rhinotracheitis virus (IBRV), bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), and parainfluenza-3 virus (PI3V) were stratified by BW and randomly assigned to 1 of 3 treatments: 1) acute immunosuppression (ACU; 0.5 mg/kg BW DEX intravenously at 1000 h only on d 0), 2) chronic immunosuppression (CHR; 0.5 mg/kg BW DEX intravenously at 1000 h on d -3 to 0), or 3) a control (CON; no DEX). On d - 4, steers were fitted with intravenous catheters in the jugular vein and placed into individual stanchions. At 1200 h on d 0, steers were administered a respiratory vaccine containing modified-live virus (MLV) isolates of IBRV, BVDV, BRSV, and PI3V and a Mannheimia haemolytica (MH) toxoid. On d 4, cattle were transported (177 km) and housed in an isolated outdoor pen. Serum was harvested on d 0, 7, 14, 21, 28, 35, 42, and 56 to determine IBRV-, BVDV-, BRSV-, and PI3V-specific antibody titers and MH whole cell and leukotoxin antibody concentrations. Sera from d -2, 0, 1, 3, 7, and 14 were used to quantify haptoglobin (Hp) concentration and ceruloplasmin (Cp) activity. Nasal swab specimens were collected on d 0, 3, and 14 to determine the presence of IBRV, BVDV, BRSV, and PI3V via PCR analysis. There was a treatment x day interaction (P < 0.01) such that CHR steers had a greater (P <= 0.07) BVDV antibody titer on d 14, 21, and 28. Moreover, IBRV-specific antibodies increased beginning on d 14 for CHR and on d 28 for ACU and remained greater through d 56 compared with CON (P = 0.03). Conversely, serum MH whole cell antibody concentration was least (P <= 0.06) for CHR from d 7 to 28 and greatest for CON (P <= 0.04) on d 56. Treatment altered Hp such that CON exhibited a greater (P < 0.01) Hp concentration than CHR but was not different from ACU (P = 0.16). On d 3, Cp was greatest for CON, intermediate for ACU, and least for CHR (treatment x day; P <= 0.01). The prevalence of IBRV and BVDV in nasal swabs on d 14 was 67 and 56%, respectively, for CHR; 10 and 10%, respectively, for CON; and 9 and 0%, respectively, for ACU (P <= 0.006). Results suggest that CHR allowed increased replication of MLV vaccine agents. Conversely, DEX-induced immunosuppression blunted the acute phase protein and antibody response against the nonreplicating MH toxoid. C1 [Richeson, J. T.; May, N. D.; Hughes, H. D.; Roberts, S. L.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX 79016 USA. [Carroll, J. A.; Sanchez, N. C. Burdick; Broadway, P. R.] ARS, Livestock Issues Res Unit, USDA, Lubbock, TX 79403 USA. [Sharon, K. P.; Ballou, M. A.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79415 USA. RP Richeson, JT (reprint author), West Texas A&M Univ, Dept Agr Sci, Canyon, TX 79016 USA. EM jricheson@wtamu.edu NR 32 TC 0 Z9 0 U1 5 U2 5 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2016 VL 94 IS 8 BP 3501 EP 3509 DI 10.2527/jas2016-0572 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PK UT WOS:000385005900040 PM 27695816 ER PT J AU Crane, AR Redden, RR Van Emon, ML Neville, TL Reynolds, LP Caton, JS Schauer, CS AF Crane, A. R. Redden, R. R. Van Emon, M. L. Neville, T. L. Reynolds, L. P. Caton, J. S. Schauer, C. S. TI Impacts of supplemental arginine on the reproductive performance of fall lambing ewes SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE amino acids; progesterone; reproduction; sheep; supplementation; weaning ID NITRIC-OXIDE; SHEEP; METABOLISM; VIABILITY; SEASON; RESTRICTION; MORTALITY; EMBRYOS; FETUS; FSH AB In sheep, embryonic and fetal death during pregnancy can account for 25% to 50% of the total number of corpora lutea (and thus potential embryos). The objective of this study was to determine the effects of injectable and oral Arg supplementation provided for 14 d postbreeding on the reproductive performance of naturally stimulated fall lambing ewes. Rambouillet ewes (n = 210) were exposed to rams equipped with marking harnesses to induce cyclicity in April 2012. Upon estrus detection (d 0) ewes were randomly assigned, in a completely random design, to 1 of 6 treatments for a 14-d treatment period: injectable saline (CON; n = 25), injectable Ala (IVALA; n = 20), injectable Arg (IVARG; n = 23), oral rumen-protected Arg (RPARG; n = 20), oral fish meal (FM; n = 24), or oral soybean meal (SBM; n = 23). Daily treatments, except CON, IVALA, and SBM, were formulated to provide supplemental Arg at 30 mg.kg BW-1.d(-1) and were provided at 0800 h daily. Ewes receiving injectable treatments were provided 454 g corn/d postinjection, whereas ewes receiving oral supplements were provided a ground ration of their respective treatments with corn individually at 0800 h daily. Plasma and serum samples were collected on d 0, 2, 4, 6, 8, 10, 12, and 14 from 12 ewes per treatment to evaluate plasma progesterone and serum AA concentrations. At lambing, birth weight, birth type, and sex were recorded. Weaning weights were recorded when the average age of lambs was 85 d. No differences (P >= 0.39) were detected for pregnancy, prolificacy, and lambing rates or lamb birth weights among treatments. However, litter weaning weight tended to be greater (P = 0.06) and weaning rates were greater (P = 0.05) in Arg-injected ewes (1.09, 0.95, 1.29, 0.72, 1.00, and 0.86, respectively). Plasma progesterone and serum Arg concentrations showed a treatment and day effect (P < 0.001), but no treatment x day interaction (P >= 0.99) was observed. In contrast to previous research, supplemental Arg during the first 14 d of pregnancy did not improve pregnancy or lambing rates; however, IVARG did positively impact weaning rates. C1 [Crane, A. R.; Neville, T. L.; Reynolds, L. P.; Caton, J. S.] North Dakota State Univ, Dept Anim Sci, Fargo, ND 58108 USA. [Crane, A. R.; Schauer, C. S.] North Dakota State Univ, Hettinger Res Extens Ctr, Hettinger, ND 58639 USA. [Crane, A. R.; Reynolds, L. P.; Caton, J. S.] North Dakota State Univ, Ctr Nutr & Pregnancy, Fargo, ND 58108 USA. [Redden, R. R.] Texas A&M AgriLife Res & Extens Ctr, San Angelo, TX 76901 USA. [Van Emon, M. L.] Montana State Univ, Dept Anim & Range Sci, USDA, ARS, Miles City, MT 59301 USA. RP Schauer, CS (reprint author), North Dakota State Univ, Hettinger Res Extens Ctr, Hettinger, ND 58639 USA. EM christopher.schauer@ndsu.edu FU North Dakota State Board of Agricultural Research and Education Research Fund-Animal Agriculture and Hay Committee FX Partial support for this research was provided by the North Dakota State Board of Agricultural Research and Education Research Fund-Animal Agriculture and Hay Committee. The authors would like to thank David Pearson, Erin Gaspar, Donald Drolc, Don Stecher, and Fernando Valdez with Kemin Industries for their assistance in this trial. NR 38 TC 0 Z9 0 U1 6 U2 6 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2016 VL 94 IS 8 BP 3540 EP 3549 DI 10.2527/jas2016-0379 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA DY3PK UT WOS:000385005900044 PM 27695817 ER PT J AU Zheng, QF Zhang, HL Mi, HY Cai, ZY Ma, ZQ Gong, SQ AF Zheng, Qifeng Zhang, Huilong Mi, Hongyi Cai, Zhiyong Ma, Zhenqiang Gong, Shaoqin TI High-performance flexible piezoelectric nanogenerators consisting of porous cellulose nanofibril (CNF)/poly(dimethylsiloxane) (PDMS) aerogel films SO NANO ENERGY LA English DT Article DE Cellulose nanofibrils (CNFs); Aerogel; Porous CNF/PDMS film; Piezoelectric materials; Nanogenerators ID HYBRID ORGANIC AEROGELS; NANOCOMPOSITE GENERATOR; DIPOLE-MOMENT; ENERGY; HYDROGEN; NANOWIRES; FIBERS; PAPER AB A novel, simple, cost-effective, and scalable technique was developed to fabricate high-performance flexible piezoelectric nanogenerators (NGs) using porous cellulose nanofibril (CNEs)/poly(dimethylsiloxane) (PDMS) aerogel film. The porous CNF/PDMS aerogel film was prepared by coating a layer of PDMS on the porous surface of a compressed CNF aerogel film produced via an environmentally friendly freeze-drying process. The porous CNF/PDMS aerogel film was then sandwiched between two thin PDMS films, followed by two aluminum foils, to form the flexible NGs. Under periodic external mechanical deformation by an oscillator, the resulting flexible porous CNF/PDMS aerogel film-based NGs exhibited very stable and high output piezoelectric signals; namely, an open-circuit voltage (V-oc) of 60.2 V, a short-circuit current (I-sc) of 10.1 mu A, and a corresponding power density of 6.3 mW/cm(3). The electric power generated by these NGs was able to directly turn on 19 blue light-emitting diodes (LEDs) and charge a capacitor up to 3.7 V. Furthermore, these NGs also demonstrated excellent stability and durability. Considering their excellent piezoelectric performance, ease of large-scale manufacturing, and environmental friendliness, this technology provides a promising solution for developing practical, flexible, and self-powered electronic devices. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Zheng, Qifeng; Gong, Shaoqin] Univ Wisconsin, Dept Mat Sci & Engn, 1509 Univ Ave, Madison, WI 53706 USA. [Zheng, Qifeng; Gong, Shaoqin] Univ Wisconsin, Wisconsin Inst Discovery, Madison, WI 53715 USA. [Zhang, Huilong; Mi, Hongyi; Ma, Zhenqiang] Univ Wisconsin, Dept Elect Engn, Madison, WI 53706 USA. [Cai, Zhiyong] USDA, Forest Prod Lab, Madison, WI 53726 USA. [Mi, Hongyi; Gong, Shaoqin] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA. RP Gong, SQ (reprint author), Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA.; Ma, ZQ (reprint author), Univ Wisconsin, Dept Elect & Comp Engn, 1415 Johnson Dr, Madison, WI 53706 USA. EM mazq@engr.wisc.edu; shaoqingong@wisc.edu FU University of Wisconsin-Madison FX The authors gratefully acknowledge the financial support of the University of Wisconsin-Madison. NR 58 TC 0 Z9 0 U1 45 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2211-2855 EI 2211-3282 J9 NANO ENERGY JI Nano Energy PD AUG PY 2016 VL 26 BP 504 EP 512 DI 10.1016/j.nanoen.2016.06.009 PG 9 WC Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied SC Chemistry; Science & Technology - Other Topics; Materials Science; Physics GA DY2FO UT WOS:000384908700059 ER PT J AU Denver, DR Brown, AMV Howe, DK Peetz, AB Zasada, IA AF Denver, Dee R. Brown, Amanda M. V. Howe, Dana K. Peetz, Amy B. Zasada, Inga A. TI Genome Skimming: A Rapid Approach to Gaining Diverse Biological Insights into Multicellular Pathogens SO PLOS PATHOGENS LA English DT Article ID PLANT-PARASITIC NEMATODES; ROOT-KNOT NEMATODES; SEQUENCE C1 [Denver, Dee R.; Brown, Amanda M. V.; Howe, Dana K.] Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. [Peetz, Amy B.; Zasada, Inga A.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. RP Denver, DR (reprint author), Oregon State Univ, Dept Integrat Biol, Corvallis, OR 97331 USA. EM denvedee@oregonstate.edu NR 24 TC 0 Z9 0 U1 4 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7366 EI 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD AUG PY 2016 VL 12 IS 8 AR e1005713 DI 10.1371/journal.ppat.1005713 PG 7 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA DW1AV UT WOS:000383376000008 PM 27490201 ER PT J AU Strunk, JL Mills, JR Ries, P Temesgen, H Jeroue, L AF Strunk, Jacob L. Mills, John R. Ries, Paul Temesgen, Hailemariam Jeroue, Lacey TI An urban forest-inventory-and-analysis investigation in Oregon and Washington SO URBAN FORESTRY & URBAN GREENING LA English DT Article DE Forest inventory; iTree eco; Landsat; Post-stratification; Urban FIA ID VEGETATION; HEALTH; TREES AB The U.S. Department of Agriculture (USDA) Forest Service, Forest Inventory and Analysis program recently inventoried trees on 257 sample plots in the urbanized areas of Oregon and Washington. Plots were located on the standard grid (approximate to 1 plot/2428 ha) and installed with the 4-subplot footprint (approximate to.067 ha with 4 circular subplots). Using these data, we examined: 1) use of the land use classification data from the National Land Cover Database (NLCD) for post-stratification; 2) the resolution of the inventory data to make inferences about subdomains (specifically sub-regions) and subgroups (species and diameter classes); and 3) the i-Tree Eco software as a tool for data compilation, estimation, and reporting. (C) 2016 Elsevier GmbH. All rights reserved. C1 [Strunk, Jacob L.] Washington State Dept Nat Resources WA DNR, Mail Stop 47014,POB 47000,1111 Washington St SE, Olympia, WA 98504 USA. [Mills, John R.] Univ Washington, USDA, Forest Serv, Pacific Northwest Res Stn, POB 3890, Portland, OR 97208 USA. [Ries, Paul] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Temesgen, Hailemariam; Jeroue, Lacey] Oregon State Univ, Dept Forest Engn Resources & Management, Peavy 204, Corvallis, OR 97331 USA. RP Strunk, JL (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, Peavy 204, Corvallis, OR 97331 USA. EM jacob.Strunk@dnr.wa.gov; JMills@fs.fed.us; Paul.Ries@oregonstate.edu; Hailemariam.Temesgen@oregonstated.edu; ljeroue@west-inc.com OI Ries, Paul D/0000-0002-9550-7167 FU Pacific Northwest Research Station of the USDA Forest Service; Oregon Department of Forestry; Oregon State University College of Forestry; American Recovery and Reinvestment Act [WFM-2619-01FHC] FX We would like to thank the Pacific Northwest Research Station of the USDA Forest Service, the Oregon Department of Forestry, and Oregon State University College of Forestry for supporting this research. Funding for the collection of the urban FIA data and for a portion of this research came from an American Recovery and Reinvestment Act of 2009 grant (WFM-2619-01FHC). We would like to thank David Nowak for providing feedback on our manuscript, and for helping us to use i-Tree Eco. We also want to acknowledge our anonymous reviewers in helping us improve the manuscript. NR 36 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1618-8667 J9 URBAN FOR URBAN GREE JI Urban For. Urban Green. PD AUG 1 PY 2016 VL 18 BP 100 EP 109 DI 10.1016/j.ufug.2016.04.006 PG 10 WC Plant Sciences; Environmental Studies; Forestry; Urban Studies SC Plant Sciences; Environmental Sciences & Ecology; Forestry; Urban Studies GA DY2HW UT WOS:000384914700012 ER PT J AU Slininger, PJ Dien, BS Kurtzman, CP Moser, BR Bakota, EL Thompson, SR O'Bryan, PJ Cotta, MA Balan, V Jin, M Sousa, LD Dale, BE AF Slininger, Patricia J. Dien, Bruce S. Kurtzman, Cletus P. Moser, Bryan R. Bakota, Erica L. Thompson, Stephanie R. O'Bryan, Patricia J. Cotta, Michael A. Balan, Venkatesh Jin, Mingjie Sousa, Leonardo da Costa Dale, Bruce E. TI Comparative Lipid Production by Oleaginous Yeasts in Hydrolyzates of Lignocellulosic Biomass and Process Strategy for High Titers SO BIOTECHNOLOGY AND BIOENGINEERING LA English DT Article DE triacylglycerol; AFEX-pretreated corn stover; dilute acid-pretreated switchgrass; biodiesel; renewable energy ID NUCLEAR-MAGNETIC-RESONANCE; HIGH ETHANOL PRODUCTIVITY; OIL PRODUCTION; TRICHOSPORON-FERMENTANS; BAGASSE HYDROLYSATE; FATTY-ACIDS; D-XYLOSE; BIODIESEL; GLUCOSE; CONVERSION AB Oleaginous yeasts can convert sugars to lipids with fatty acid profiles similar to those of vegetable oils, making them attractive for production of biodiesel. Lignocellulosic biomass is an attractive source of sugars for yeast lipid production because it is abundant, potentially low cost, and renewable. However, lignocellulosic hydrolyzates are laden with byproducts which inhibit microbial growth and metabolism. With the goal of identifying oleaginous yeast strains able to convert plant biomass to lipids, we screened 32 strains from the ARS Culture Collection, Peoria, IL to identify four robust strains able to produce high lipid concentrations from both acid and base-pretreated biomass. The screening was arranged in two tiers using undetoxified enzyme hydrolyzates of ammonia fiber expansion (AFEX)-pretreated cornstover as the primary screening medium and acid-pretreated switch grass as the secondary screening medium applied to strains passing the primary screen. Hydrolyzates were prepared at similar to 18-20% solids loading to provide similar to 110 g/L sugars at similar to 56:39:5 mass ratio glucose: xylose:arabinose. A two stage process boosting the molar C:N ratio from 60 to well above 400 in undetoxified switchgrass hydrolyzate was optimized with respect to nitrogen source, C:N, and carbon loading. Using this process three strains were able to consume acetic acid and nearly all available sugars to accumulate 50-65% of cell biomass as lipid (w/w), to produce 25-30 g/L lipid at 0.12-0.22 g/L/h and 0.13-0.15 g/g or 39-45% of the theoretical yield at pH 6 and 7, a performance unprecedented in lignocellulosic hydrolyzates. Three of the top strains have not previously been reported for the bioconversion of lignocellulose to lipids. The successful identification and development of top-performing lipid-producing yeast in lignocellulose hydrolyzates is expected to advance the economic feasibility of high quality biodiesel and jet fuels from renewable biomass, expanding the market potential for lignocellulose-derived fuels beyond ethanol for automobiles to the entire U.S. transportation market. (C) 2016 Wiley Periodicals, Inc. C1 [Slininger, Patricia J.; Dien, Bruce S.; Kurtzman, Cletus P.; Moser, Bryan R.; Bakota, Erica L.; Thompson, Stephanie R.; O'Bryan, Patricia J.; Cotta, Michael A.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Balan, Venkatesh; Jin, Mingjie; Sousa, Leonardo da Costa; Dale, Bruce E.] Michigan State Univ, DOE Great Lakes Bioenergy Res Ctr, Lansing, MI USA. RP Slininger, PJ (reprint author), ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM Pat.Slininger@ars.usda.gov OI Jin, Mingjie/0000-0002-9493-305X; Dien, Bruce/0000-0003-3863-6664 NR 59 TC 3 Z9 3 U1 13 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0006-3592 EI 1097-0290 J9 BIOTECHNOL BIOENG JI Biotechnol. Bioeng. PD AUG PY 2016 VL 113 IS 8 BP 1676 EP 1690 DI 10.1002/bit.25928 PG 15 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DX9XU UT WOS:000384751100007 PM 26724417 ER PT J AU Han, YH Pan, JS Thammapichai, P Li, ZY Weng, YQ AF Han, Yonghua Pan, Junsong Thammapichai, Paradee Li, Zongyun Weng, Yiqun TI Asynchronous meiosis in Cucumis hystrix-cucumber synthetic tetraploids resulting in low male fertility SO CROP JOURNAL LA English DT Article DE Cucumber; Cucumis hystrix; Amphidiploid; Meiosis; Asynchrony ID IN-SITU HYBRIDIZATION; BRASSICA-NAPUS L; INTERSPECIFIC HYBRIDIZATION; CHROMOSOME ELIMINATION; GENOMIC CHANGES; SATIVUS L.; POLYPLOIDS; HYBRIDS; ARABIDOPSIS; POACEAE AB Interspecific hybridization and allopolyploidization contribute to the improvement of many important crops. Recently, we successfully developed an amphidiploid from an interspecific cross between cucumber (Cucumis sativus, 2n = 2x = 14) and its relative C. hystrix (2n = 2x = 24) followed by chemical induction of chromosome doubling. The resulting allotetraploid plant was self-pollinated for three generations. The fertility and seed set of the amphidiploid plants were very low. In this study, we investigated the meiotic chromosome behavior in pollen mother cells with the aid of fluorescence in situ hybridization, aiming to identify the reasons for the low fertility and seed set in the amphidiploid plants. Homologous chromosome pairing appeared normal, but chromosome laggards were common, owing primarily to asynchronous meiosis of chromosomes from the two donor genomes. We suggest that asynchronous meiotic rhythm between the two parental genomes is the main reason for the low fertility and low seed set of the C. hystrix-cucumber amphidiploid plants. (C) 2016 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. C1 [Han, Yonghua; Li, Zongyun] Jiangsu Normal Univ, Sch Life Sci, Inst Integrat Plant Biol, Xuzhou, Peoples R China. [Han, Yonghua; Pan, Junsong; Thammapichai, Paradee; Weng, Yiqun] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Pan, Junsong] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai, Peoples R China. [Weng, Yiqun] USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Han, YH (reprint author), Jiangsu Normal Univ, Sch Life Sci, Inst Integrat Plant Biol, Xuzhou, Peoples R China.; Weng, YQ (reprint author), USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. EM hanyonghua@jsnu.edu.cn; yiqun.weng@wisc.edu NR 35 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD AUG PY 2016 VL 4 IS 4 BP 275 EP 279 DI 10.1016/j.cj.2016.05.003 PG 5 WC Agronomy SC Agriculture GA DX7BA UT WOS:000384539600004 ER PT J AU Fang, WP Meinhardt, LW Tan, HW Zhou, L Mischke, S Wang, XH Zhang, DP AF Fang, Wanping Meinhardt, Lyndel W. Tan, Huawei Zhou, Lin Mischke, Sue Wang, Xinghua Zhang, Dapeng TI Identification of the varietal origin of processed loose-leaf tea based on analysis of a single leaf by SNP nanofluidic array SO CROP JOURNAL LA English DT Article DE Authentication; Camellia sinensis; Conservation; Food adulteration; Molecular markers ID INFRARED REFLECTANCE SPECTROSCOPY; CAMELLIA-SINENSIS L.; GREEN TEA; GENETIC DIVERSITY; CULTIVAR IDENTIFICATION; PHYLOGENETIC TREES; TOTAL POLYPHENOLS; SSR MARKERS; POLYMORPHISMS; GERMPLASM AB Tea is an important cash crop, representing a $40 billion-a-year global market. Differentiation of the tea market has resulted in increasing demand for tea products that are sustainably and responsibly produced. Tea authentication is important because of growing concerns about fraud involving premium tea products. Analytical technologies are needed for protection and value enhancement of high-quality brands. For loose-leaf teas, the challenge is that the authentication needs to be established on the basis of a single leaf, so that the products can be traced back to the original varieties. A new generation of molecular markers offers an ideal solution for authentication of processed agricultural products. Using a nanofluidic array to identify variant SNP sequences, we tested genetic identities using DNA extracted from single leaves of 14 processed commercial tea products. Based on the profiles of 60 SNP markers, the genetic identity of each tea sample was unambiguously identified by multilocus matching and ordination analysis. Results for repeated samples of multiple tea leaves from the same products (using three independent DNA extractions) showed 100% concordance, showing that the nanofluidic system is a reliable platform for generating tea DNA fingerprints with high accuracy. The method worked well on green, oolong, and black teas, and can handle a large number of samples in a short period of time. It is robust and cost-effective, thus showing high potential for practical application in the value chain of the tea industry. Production and hosting by Elsevier B.V. on behalf of Crop Science Society of China and Institute of Crop Science, CAAS. C1 [Fang, Wanping; Tan, Huawei; Zhou, Lin] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Fang, Wanping; Meinhardt, Lyndel W.; Mischke, Sue; Zhang, Dapeng] USDA ARS, Sustainable Perennial Crops Lab, 10300 Baltimore Ave,Bldg 001,Rm 223,BARC W, Beltsville, MD 20705 USA. [Wang, Xinghua] Yunnan Puer Tea Seeds Propagat & Extens Unit, Puer 665000, Yunnan, Peoples R China. RP Zhang, DP (reprint author), USDA ARS, Sustainable Perennial Crops Lab, 10300 Baltimore Ave,Bldg 001,Rm 223,BARC W, Beltsville, MD 20705 USA. EM Dapeng.Zhang@ars.usda.gov NR 58 TC 1 Z9 1 U1 9 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2095-5421 EI 2214-5141 J9 CROP J JI Crop J. PD AUG PY 2016 VL 4 IS 4 BP 304 EP 312 DI 10.1016/j.cj.2016.02.001 PG 9 WC Agronomy SC Agriculture GA DX7BA UT WOS:000384539600007 ER PT J AU Bigi, MM Blanco, FC Araujo, FR Thacker, TC Zumarraga, MJ Cataldi, AA Soria, MA Bigi, F AF Bigi, Maria M. Carlos Blanco, Federico Araujo, Flabio R. Thacker, Tyler C. Zumarraga, Martin J. Cataldi, Angel A. Soria, Marcelo A. Bigi, Fabiana TI Polymorphisms of 20 regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis SO MICROBIOLOGY AND IMMUNOLOGY LA English DT Article DE Mycobacterium bovis; Mycobacterium tuberculosis; polymorphism; regulator ID COMPLETE GENOME SEQUENCE; NITRATE REDUCTION; VIRULENCE FACTORS; CELL-DIVISION; KINASE; PHOSPHORYLATION; GENE; EXPRESSION; PKND; PHOSPHATASE AB Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans and animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and other members of the MTC evolved. The genome of M. bovis is over >99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In the present study, we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutations in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis. C1 [Bigi, Maria M.; Soria, Marcelo A.] UBA, Sch Agron, RA-1417 Buenos Aires, DF, Argentina. [Carlos Blanco, Federico; Zumarraga, Martin J.; Cataldi, Angel A.; Bigi, Fabiana] Natl Inst Agr Technol INTA, Biotechnol Inst, RA-1686 Hurlingham, Argentina. [Araujo, Flabio R.] EMBRAPA, BR-8605 Campo Grande, MS, Brazil. [Thacker, Tyler C.] ARS, USDA, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. RP Bigi, F (reprint author), Natl Inst Agr Technol INTA, Biotechnol Inst, RA-1686 Hurlingham, Argentina. EM bigi.fabiana@inta.gob.ar FU INTA [PNBIO1131034]; ANPCyT PICT [2014-3637] FX This work was funded by INTA grant PNBIO1131034 and ANPCyT grant PICT (2014-3637). BFC, KLI and BF are CONICET fellows. We are grateful to Julia Sabio y Garcia for critical reading of the manuscript. We thank Luis Fernandez for the bibliography provided. NR 51 TC 1 Z9 1 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0385-5600 EI 1348-0421 J9 MICROBIOL IMMUNOL JI Microbiol. Immunol. PD AUG PY 2016 VL 60 IS 8 BP 552 EP 560 DI 10.1111/1348-0421.12402 PG 9 WC Immunology; Microbiology SC Immunology; Microbiology GA DX9UI UT WOS:000384741200005 PM 27427512 ER PT J AU Gordon, IJ Altwegg, R Evans, DM Ewen, JG Johnson, JA Pettorelli, N Young, JK AF Gordon, I. J. Altwegg, R. Evans, D. M. Ewen, J. G. Johnson, J. A. Pettorelli, N. Young, J. K. TI Reducing agricultural loss and food waste: how will nature fare? SO ANIMAL CONSERVATION LA English DT Editorial Material ID BIRDS; GULLS; FISH; MANAGEMENT; MORTALITY; CONFLICT; SECURITY; SEABIRDS; REFUSE; REGION C1 [Gordon, I. J.] James Cook Univ, Div Trop Environm & Soc, Townsville, Qld 4811, Australia. [Altwegg, R.] Univ Cape Town, Dept Stat Sci, Stat Ecol Environm & Conservat, Rondebosch, South Africa. [Altwegg, R.] Univ Cape Town, African Climate & Dev Initiat, Rondebosch, South Africa. [Evans, D. M.] Newcastle Univ, Sch Biol, Newcastle Upon Tyne, Tyne & Wear, England. [Ewen, J. G.; Pettorelli, N.] Zool Soc London, Inst Zool, London, England. [Johnson, J. A.] Univ North Texas, Inst Appl Sci, Dept Biol Sci, Denton, TX USA. [Young, J. K.] Utah State Univ, Dept Wildland Resources, USDA,Wildlife Serv, Natl Wildlife Res Ctr,Predator Res Facil, Logan, UT 84322 USA. RP Gordon, IJ (reprint author), James Cook Univ, Div Trop Environm & Soc, Townsville, Qld 4811, Australia. EM iain.gordon@jcu.edu.au NR 42 TC 0 Z9 0 U1 6 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1367-9430 EI 1469-1795 J9 ANIM CONSERV JI Anim. Conserv. PD AUG PY 2016 VL 19 IS 4 BP 305 EP 308 DI 10.1111/acv.12290 PG 4 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DT0YH UT WOS:000381208400001 ER PT J AU Callaham, MA AF Callaham, Mac A., Jr. TI Introduction SO APPLIED SOIL ECOLOGY LA English DT Editorial Material C1 [Callaham, Mac A., Jr.] US Forest Serv, USDA, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA USA. RP Callaham, MA (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA USA. NR 2 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD AUG PY 2016 VL 104 BP 1 EP 2 DI 10.1016/j.apsoil.2016.03.022 PG 2 WC Soil Science SC Agriculture GA DX2XT UT WOS:000384237600001 ER PT J AU Butt, KR Callaham, M Loudermilk, EL Blaik, R AF Butt, Kevin R. Callaham, Mac A., Jr. Loudermilk, E. Louise Blaik, Rowan TI Action of earthworms on flint burial - A return to Darwin's estate SO APPLIED SOIL ECOLOGY LA English DT Article; Proceedings Paper CT 10th International Symposium on Earthworm Ecology (ISEE) CY JUN, 2014 CL Athens, GA DE Bioturbation; Charles Darwin; Casting; Down House; Soil ID SOIL; BIOTURBATION; CULTURE AB For thirty years, from the early 1840s, Charles Darwin documented the disappearance of flints in the grounds of Down House in Kent, at a location originally known as the "Stony Field". This site (Great Pucklands Meadow - GPM) was visited in 2007 and an experiment set up in this ungrazed grassland. Locally-sourced flints (either large - 12 cm, or small - 5 cm dia.) were deposited at two densities within sixteen 1 m(2) plots in a randomised factorial design. The area selected was distant from public access routes and remained unmown throughout the duration here reported. Fixed point photographs were taken at the outset to enable later photogrammetric analysis. After 6 years, the site was re-examined. The flints had generally been incorporated into the soil. Photographs were re-taken, proportion of buried flints recorded and measurements made of burial depth from a quarter of each plot. Results showed that large flints were more deeply incorporated than smaller (p = 0.025), but more of the latter were below the soil surface. A controlled laboratory experiment was also conducted using Aporrectodea longa (the dominant earthworm species in GPM) to assess effects of casting in the absence of other biota. Results suggested that this species has a major influence on flint burial through surface casting. Combined with a long term, but small scale collection of A. longa casts from an area close to GPM, all results were consistent with those provided by Darwin and showed that rate of flint burial was within the range of 0.21-0.96 cm y(-1). (C) 2015 Elsevier B.V. All rights reserved. C1 [Butt, Kevin R.] Univ Cent Lancashire, Preston PR1 2HE, Lancs, England. [Callaham, Mac A., Jr.; Loudermilk, E. Louise] US Forest Serv, USDA, Southern Res Stn, Athens, GA USA. [Blaik, Rowan] English Heritage, Down House, Downe BR6 7JT, Kent, England. RP Butt, KR (reprint author), Univ Cent Lancashire, Preston PR1 2HE, Lancs, England. EM krbutt@uclan.ac.uk OI Butt, Kevin/0000-0003-0886-7795 NR 16 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD AUG PY 2016 VL 104 BP 157 EP 162 DI 10.1016/j.apsoil.2015.04.002 PG 6 WC Soil Science SC Agriculture GA DX2XT UT WOS:000384237600020 ER PT J AU Huang, Y Mao, K Chen, X Kawabe, T Zhu, J Urban, JF Germain, RN Paul, WE AF Huang, Y. Mao, K. Chen, X. Kawabe, T. Zhu, J. Urban, Jr J. F. Germain, R. N. Paul, W. E. TI Tissue-resident ILC2 and inflammatory ILC2: two distinct ILC populations SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT International Congress of Immunology (ICI) CY AUG 21-26, 2016 CL Melbourne, AUSTRALIA C1 [Huang, Y.; Chen, X.; Kawabe, T.; Zhu, J.; Paul, W. E.] NIAID, NIH, Immunol Lab, 9000 Rockville Pike, Bethesda, MD 20892 USA. [Mao, K.; Germain, R. N.] NIAID, NIH, Lab Syst Biol, 9000 Rockville Pike, Bethesda, MD 20892 USA. [Urban, Jr J. F.] ARS, Beltsville Human Nutr Res Ctr, USDA, Genom & Immunol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0014-2980 EI 1521-4141 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD AUG PY 2016 VL 46 SU 1 SI SI MA 4728 BP 297 EP 297 PG 1 WC Immunology SC Immunology GA DW4KA UT WOS:000383610400599 ER PT J AU Sun, XH Wang, H Qian, ML Zhao, Y Zhuang, Y Urban, J Fung, KM AF Sun, X-H Wang, H. Qian, M. L. Zhao, Y. Zhuang, Y. Urban, J., Jr. Fung, K-M TI Down-regulating E protein function augments ILC2 production in the thymus SO EUROPEAN JOURNAL OF IMMUNOLOGY LA English DT Meeting Abstract CT International Congress of Immunology (ICI) CY AUG 21-26, 2016 CL Melbourne, AUSTRALIA C1 [Sun, X-H; Wang, H.; Qian, M. L.; Zhao, Y.] Oklahoma Med Res Fdn, 825 NE 13th St, Oklahoma City, OK 73104 USA. [Zhuang, Y.] Duke Univ, Med Ctr, Durham, NC 27706 USA. [Urban, J., Jr.] ARS, USDA, Bettsville Human Nutr Ctr, Bettsville, OH USA. [Fung, K-M] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0014-2980 EI 1521-4141 J9 EUR J IMMUNOL JI Eur. J. Immunol. PD AUG PY 2016 VL 46 SU 1 SI SI MA 1133 BP 1066 EP 1066 PG 1 WC Immunology SC Immunology GA DW4KA UT WOS:000383610402539 ER PT J AU Silva, CA Klauberg, C Hudak, AT Vierling, LA Liesenberg, V Carvalho, SPCE Rodriguez, LCE AF Silva, Carlos Alberto Klauberg, Carine Hudak, Andrew T. Vierling, Lee A. Liesenberg, Veraldo Carvalho, Samuel P. C. e Rodriguez, Luiz C. E. TI A principal component approach for predicting the stem volume in Eucalyptus plantations in Brazil using airborne LiDAR data SO FORESTRY LA English DT Article DE supply chain; LiDAR metrics; remote sensing; Eucalyptus spp; forest management; multivariate statistics ID DISCRETE-RETURN LIDAR; LASER SCANNER DATA; FOREST STANDS; TREE HEIGHT; BIOMASS; PARAMETERS; REGRESSION; INTENSITY; SAMPLES AB Improving management practices in industrial forest plantations may increase production efficiencies, thereby reducing pressures on native tropical forests for meeting global pulp needs. This study aims to predict stem volume (V) in plantations of fast-growing Eucalyptus hybrid clones located in southeast Brazil using field plot and airborne Light Detection and Ranging (LiDAR) data. Forest inventory attributes and LiDAR-derived metrics were calculated at 108 sample plots. The best LiDAR-based predictors of V were identified based on loadings calculated from a principal component analysis (PCA). After selecting these best predictors using PCA, we developed multiple regression models predicting V from selected LiDAR metrics. Metrics related to tree height and canopy depth were most effective for V prediction, with an overall model coefficient of determination (adj. R-2) of 0.87, and a root mean squared error (RMSE) of 27.60 m(3) ha(-1) (i.e. relative RMSE = 9.99 per cent). We used this model to map stem V of Eucalyptus hybrid clones across the full LiDAR data extent. The accuracy and precision of our results show that LiDAR-derived V is appropriate for updating Eucalyptus forest base maps and registries in the paper and pulp supply chain. However, further studies are necessary to evaluate and compare the cost of acquisition and processing of LiDAR data against conventional V inventory in this system. C1 [Silva, Carlos Alberto; Klauberg, Carine; Hudak, Andrew T.] US Forest Serv, USDA, Rocky Mt Res Stn, RMRS, 1221 South Main St, Moscow, ID 83843 USA. [Silva, Carlos Alberto; Vierling, Lee A.] Univ Idaho, Dept Forest Rangeland & Fire Sci, Coll Nat Resources, 875 Perimeter Dr, Moscow, ID 83843 USA. [Liesenberg, Veraldo] Santa Catarina State Univ UDESC, Ave Luiz de Camoes 2090, BR-88520000 Lages, SC, Brazil. [Carvalho, Samuel P. C. e; Rodriguez, Luiz C. E.] Univ Sao Paulo, Dept Forest Sci, Coll Agr Luiz de Queiroz ESALQ, Ave Padua Dias 11,POB 09, BR-13418900 Piracicaba, SP, Brazil. RP Silva, CA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, RMRS, 1221 South Main St, Moscow, ID 83843 USA.; Silva, CA (reprint author), Univ Idaho, Dept Forest Rangeland & Fire Sci, Coll Nat Resources, 875 Perimeter Dr, Moscow, ID 83843 USA. EM csilva@uidaho.edu RI CARVALHO, SAMUEL/F-8339-2014 OI CARVALHO, SAMUEL/0000-0002-5590-9049 FU International Programs of the US Forest Service (USFS); State of Sao Paulo Research Foundation-FAPESP [12/03176-0, 13/05081-9]; FIBRIA S/A, a Brazilian pulp and paper company FX This research was possible due to the support of International Programs of the US Forest Service (USFS) during an internship by the first author at the USFS Rocky Mountain Research Station Forestry Sciences Laboratory in Moscow, Idaho. This research was supported by The State of Sao Paulo Research Foundation-FAPESP (Process 12/03176-0 and 13/05081-9). LiDAR and field inventory data collections were funded by FIBRIA S/A, a Brazilian pulp and paper company. NR 56 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD AUG PY 2016 VL 89 IS 4 BP 422 EP 433 DI 10.1093/forestry/cpw016 PG 12 WC Forestry SC Forestry GA DW8IR UT WOS:000383899000010 ER PT J AU Xu, Q Guerrero, FD Palavesam, A de Leon, AAP AF Xu, Qiang Guerrero, Felix D. Palavesam, Azhahianambi de Leon, Adalberto A. Perez TI Use of electroporation as an option to transform the horn fly, Haematobia irritans: a species recalcitrant to microinjection SO INSECT SCIENCE LA English DT Article DE DsRed; EGFP; electroporation; Haematobia irritans; horn fly; transformation; transgenic insect ID GERM-LINE TRANSFORMATION; GENETIC-TRANSFORMATION; INSECT TRANSGENESIS; AEDES-AEGYPTI; PIGGYBAC; DROSOPHILA; VECTOR; EXPRESSION; TRANSPOSON; MOSQUITO AB The horn fly, Haematobia irritans, is a serious pest of cattle in North America. The control of horn flies has primarily relied on insecticides. However, the heavy use of insecticides has led to the development of insecticide resistance in horn flies. Novel methods to control horn flies are greatly needed. Transgenic technology is an effective tool to genetically modify insects and may lead to novel methods of pest control based on genomic approaches. Here we report a piggyBac-mediated transformation of the horn fly via electroporation. Transformation with a DsRed fluorescent marker protein coding region was verified by PCR analysis of individual fly bodies and pupal cases and sequencing of PCR products. However, Southern blot analysis failed to indicate the DsRed gene was integrated into the horn fly genome. Thus, the electroporation protocol may have caused the DsRed gene to be integrated into bacterial symbionts of the horn fly. C1 [Xu, Qiang] Abilene Christian Univ, Dept Biol, ACU Box 27868, Abilene, TX 79699 USA. [Guerrero, Felix D.; Palavesam, Azhahianambi; de Leon, Adalberto A. Perez] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. [Guerrero, Felix D.; de Leon, Adalberto A. Perez] ARS, USDA, Vet Pest Genom Ctr, Kerrville, TX 78028 USA. [Palavesam, Azhahianambi] Tamil Nadu Vet & Anim Sci Univ, Madras Vet Coll, Dept Vet Parasitol, Madras, Tamil Nadu, India. RP Xu, Q (reprint author), Abilene Christian Univ, Dept Biol, ACU Box 27868, Abilene, TX 79699 USA. EM qxx07a@acu.edu RI Palavesam, Azhahianambi/K-7606-2015 OI Palavesam, Azhahianambi/0000-0002-7108-6767 FU U.S. Department of Agriculture-APHIS [58-6205-0-038]; Abilene Christian University Math and Science Grants FX We acknowledge Kylie Bendele, Kathy Li, Matt Waldon, and Younghyup Byun for helping keep the horn fly colony and Dr. Lane Foil, Department of Entomology, Louisiana State University, for helpful discussion on horn fly biology. We also thank Dr. Max Scott, Department of Genetics, North Carolina State University, Raleigh, NC, and Dr. Al Handler, U.S. Department of Agriculture-Agricultural Research Service, Gainesville, FL, USA for providing plasmids. This work was supported by U.S. Department of Agriculture-APHIS Cooperative Agreement Fund 58-6205-0-038 and Abilene Christian University Math and Science Grants. USDA is an equal opportunity provider and employer. NR 40 TC 0 Z9 0 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9609 EI 1744-7917 J9 INSECT SCI JI Insect Sci. PD AUG PY 2016 VL 23 IS 4 BP 621 EP 629 DI 10.1111/1744-7917.12207 PG 9 WC Entomology SC Entomology GA DX2CW UT WOS:000384175700013 PM 25645001 ER PT J AU Seo, J Hosteng, TK Phares, BM Wacker, JP AF Seo, Junwon Hosteng, Travis K. Phares, Brent M. Wacker, James P. TI Live-Load Performance Evaluation of Historic Covered Timber Bridges in the United States SO JOURNAL OF PERFORMANCE OF CONSTRUCTED FACILITIES LA English DT Article DE Field testing; Live-load performance; Timber; Covered bridges; Burr arch truss; Howe truss; Queen post truss ID HEALTH MONITORING-SYSTEM; FATIGUE; MODELS AB The National Historic Covered Bridge Preservation Program (NHCBP), sponsored by the Federal Highway Administration (FHWA), was established to preserve the covered timber bridge structures that were constructed in the early 1800s. Today, many of the approximately 880 covered timber bridges still in existence in the United States are closed to vehicular traffic; furthermore, a large percentage of the remaining bridges open to traffic are restricted by load postings. Unfortunately, there are no current load-rating standards for covered timber bridges, so engineers do not have many resources at their disposal to reliably understand the behavior of these complex structures. As a result, the estimated load postings and/or ratings are often too conservative. To better understand the live-load performance of covered timber bridges and to develop improved criteria for their load ratings, a series of live-load tests were performed on 11 single-span, historic covered timber bridges. This paper explains the field testing conducted on all of the bridges, and makes recommendations for the conduct of other similar tests. The tests consisted of installing a network of multiple displacement and strain sensors on the structures, and monitoring global displacements and member strains at various cross sections during passage of a known test. The vehicle used in the testing met the load restrictions in place at the time of testing. The results of this work serve as a basis for instrumentation and field testing, to investigate the live-load performance on such bridges. This paper outlines the field-testing methods and summarizes the results from the testing of all 11 bridges. Based on the field-testing methods, a field-testing protocol is recommended for the live-load testing of historic covered timber bridges. Feasibility of the recommended protocol is also evaluated using a finite-element model analysis for a selected bridge among the tested bridges. (C) 2015 American Society of Civil Engineers. C1 [Seo, Junwon] South Dakota State Univ, Dept Civil & Environm Engn, Brookings, SD 57007 USA. [Hosteng, Travis K.] Natl Ctr Wood Transportat Struct, 2711 S Loop Dr,Suite 4700, Ames, IA 50010 USA. [Phares, Brent M.] Iowa State Univ, Bridge Engn Ctr, 2711 S Loop Dr,Suite 4700, Ames, IA 50010 USA. [Wacker, James P.] US Forest Serv, USDA, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. RP Seo, J (reprint author), South Dakota State Univ, Dept Civil & Environm Engn, Brookings, SD 57007 USA. EM junwon.seo@sdstate.edu; kickhos@iastate.edu; bphares@iastate.edu; jwacker@fs.fed.us FU Federal Highway Administration; USDA Forest Products Laboratory (FPL) under the National Historic Covered Bridge Preservation Program FX This research was sponsored by the Federal Highway Administration and the USDA Forest Products Laboratory (FPL) under the National Historic Covered Bridge Preservation Program. The authors especially thank Doug Rammer from the FPL for his cooperation and assistance during the field testing of the covered bridges. NR 20 TC 0 Z9 0 U1 3 U2 3 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0887-3828 EI 1943-5509 J9 J PERFORM CONSTR FAC JI J. Perform. Constr. Facil. PD AUG PY 2016 VL 30 IS 4 AR 04015094 DI 10.1061/(ASCE)CF.1943-5509.0000852 PG 13 WC Construction & Building Technology; Engineering, Civil SC Construction & Building Technology; Engineering GA DS9DA UT WOS:000381081500038 ER PT J AU Mound, L Lima, E O'Donnell, C Cavalleri, A AF Mound, Laurence Lima, Elison O'Donnell, Cheryle Cavalleri, Adriano TI The New World grass-thrips genus Plesiothrips (Thysanoptera: Thripidae) and its palaeotropical relationships SO AUSTRAL ENTOMOLOGY LA English DT Article DE grass feeding; identification; neotropics; Plesiothrips perplexus ID GENERA AB Morphological character states displayed by Plesiothrips Hood, a New World genus of grass-living thrips, are assessed. This assessment indicates that Plesiothrips shares more character states with genera from Southeast Asia, including the Trichromothrips genus-group, than with any New World Thripinae. Sorghothrips sakagami (Kudo) comb. n. is removed from Plesiothrips; Taeniothrips aberrans Crawford syn. n. is considered a new synonym of Plesiothrips amblycauda Hood; and an illustrated identification key is given to 16 Plesiothrips species for which females are known. C1 [Mound, Laurence] CSIRO, Australian Natl Insect Collect, GPO Box 1700, Canberra, ACT 2601, Australia. [Lima, Elison] Univ Fed Piaui, Campus Amilcar Ferreira Sobral, Floriano, PI, Brazil. [O'Donnell, Cheryle] USDA APHIS PPQ NIS, Beltsville, MD USA. [Cavalleri, Adriano] Univ Fed Rio Grande, Sao Lourenco Do Sul, RS, Brazil. RP Mound, L (reprint author), CSIRO, Australian Natl Insect Collect, GPO Box 1700, Canberra, ACT 2601, Australia. EM laurence.mound@csiro.au RI Cavalleri, Adriano/D-3264-2015; Mound, Laurence/A-3967-2009 OI Cavalleri, Adriano/0000-0003-4163-6745; NR 24 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2052-1758 EI 2052-174X J9 AUSTRAL ENTOMOL JI Austral Entomol. PD AUG PY 2016 VL 55 IS 3 BP 340 EP 346 DI 10.1111/aen.12198 PG 7 WC Entomology SC Entomology GA DW1BL UT WOS:000383377600016 ER PT J AU Contreras-Govea, FE Muck, RE Weimer, PJ Hymes-Fecht, UC AF Contreras-Govea, F. E. Muck, R. E. Weimer, P. J. Hymes-Fecht, U. C. TI In vitro ruminal fermentation of treated alfalfa silage using ruminal inocula from high and low feed-efficient lactating cows SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE additives; alfalfa silage additives; bacteria; feed efficiency; in vitro ruminal fermentation; low and high feed efficiency cows; rumen; silage ID DAIRY-COWS; CONVERSION EFFICIENCY; BACTERIAL COMMUNITY; ANIMAL NUTRITION; DETERGENT FIBER; NITROGEN; ACID; EMISSIONS; PRODUCTS; QUALITY AB Aims: To assess the effect of two additives on alfalfa silage and on in vitro ruminal fermentation when using ruminal inocula from high feed-efficient (HE) and low feed-efficient (LE) lactating cows. Methods and Results: First-and second-cut alfalfa was harvested at 40% bloom stage, treated with control (no additive), Lactobacillus plantarum (LP) or formic acid (Formic), ensiled in 1 +/- 0 l minisilos, and fermented for 60 days. Fermented alfalfa was incubated in vitro for 24 h using ruminal inoculum from HE and LE lactating cows. The pH was lower in alfalfa silage treated with LP and Formic, and produced lower ammonia-N than did the control. In vitro true dry matter digestibility (IVTDMD) was higher with ruminal inoculum from HE than LE cows, but there was no consistent effect of treated alfalfa on microbial biomass yield and in vitro volatile fatty acids. Conclusions: The IVTDMD was numerically greater with ruminal inoculum from higher feed-efficient cows although statistical significance was only demonstrated with the first-cut alfalfa. However, treated alfalfa silage did not show the effect expected on in vitro microbial biomass yield. Significance and Impact of the Study: The feed efficiency of cows used as a source of ruminal inocula may affect IVTDMD and be a source of variation across in vitro runs. Differences in ruminal fermentation between cows of different feed efficiency could help to explain differences in milk yield and other parameters of dairy cattle performance. C1 [Contreras-Govea, F. E.] Univ Wisconsin, Dept Dairy Sci, 1675 Observ Dr,266 Anim Sci Bldg, Madison, WI 53706 USA. [Muck, R. E.; Weimer, P. J.; Hymes-Fecht, U. C.] USDA, Dairy Forage Res Ctr, Madison, WI USA. RP Contreras-Govea, FE (reprint author), Univ Wisconsin, Dept Dairy Sci, 1675 Observ Dr,266 Anim Sci Bldg, Madison, WI 53706 USA. EM contreras@wisc.edu FU USDA-Agricultural Research Service CRIS Projects [3655-31000-023-00D, 3655-31000-024-00D] FX This research was supported by USDA-Agricultural Research Service CRIS Projects 3655-31000-023-00D and 3655-31000-024-00D. We thank the USDFRC barn crew for animal care and handling. NR 35 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 EI 1365-2672 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD AUG PY 2016 VL 121 IS 2 BP 333 EP 340 DI 10.1111/jam.13103 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DW1LL UT WOS:000383404600003 PM 27241014 ER PT J AU Singer, SD Chen, GQ Mietkiewska, E Tomasi, P Jayawardhane, K Dyer, JM Weselake, RJ AF Singer, Stacy D. Chen, Guanqun Mietkiewska, Elzbieta Tomasi, Pernell Jayawardhane, Kethmi Dyer, John M. Weselake, Randall J. TI Arabidopsis GPAT9 contributes to synthesis of intracellular glycerolipids but not surface lipids SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Acyl-CoA specificity; acyl lipid biosynthesis; cutin; GPAT; lipid droplet; pollen grain; regio-specificity; surface wax ID GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE; BRASSICA-NAPUS; DIACYLGLYCEROL ACYLTRANSFERASE; TRIACYLGLYCEROL BIOSYNTHESIS; SACCHAROMYCES-CEREVISIAE; PLANT TRANSFORMATION; OIL ACCUMULATION; SN-2 PREFERENCE; GENE-EXPRESSION; IDENTIFICATION AB Arabidopsis glycerol-3-phosphate acyltransferase 9 (GPAT9) is an sn-1 specific acyl-CoA:GPAT that contributes to intracellular glycerolipid biosynthesis in seeds, developing leaves and pollen grains, but not to extracellular glycerolipid biosynthesis.GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (GPAT) genes encode enzymes involved in glycerolipid biosynthesis in plants. Ten GPAT homologues have been identified in Arabidopsis. GPATs 4-8 have been shown to be involved in the production of extracellular lipid barrier polyesters. Recently, GPAT9 was reported to be essential for triacylglycerol (TAG) biosynthesis in developing Arabidopsis seeds. The enzymatic properties and possible functions of GPAT9 in surface lipid, polar lipid and TAG biosynthesis in non-seed organs, however, have not been investigated. Here we show that Arabidopsis GPAT9 exhibits sn-1 acyltransferase activity with high specificity for acyl-coenzyme A, thus providing further evidence that this GPAT is involved in storage lipid biosynthesis. We also confirm a role for GPAT9 in seed oil biosynthesis and further demonstrate that GPAT9 contributes to the biosynthesis of both polar lipids and TAG in developing leaves, as well as lipid droplet production in developing pollen grains. Conversely, alteration of constitutive GPAT9 expression had no obvious effects on surface lipid biosynthesis. Taken together, these studies expand our understanding of GPAT9 function to include modulation of several different intracellular glycerolipid pools in plant cells. C1 [Singer, Stacy D.; Chen, Guanqun; Mietkiewska, Elzbieta; Jayawardhane, Kethmi; Weselake, Randall J.] Univ Alberta, Agr Lipid Biotechnol Program, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. [Tomasi, Pernell; Dyer, John M.] USDA ARS, US Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. [Chen, Guanqun] Univ Manitoba, Dept Biol Sci, 50 Sifton Rd, Winnipeg, MB R3T 2N2, Canada. RP Weselake, RJ (reprint author), Univ Alberta, Agr Lipid Biotechnol Program, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada. EM randall.weselake@ualberta.ca FU Alberta Innovates Bio Solutions; Alberta Enterprise and Advance Education; Canada Foundation for Innovation; Canada Research Chairs Program; Natural Sciences and Engineering Research Council of Canada FX The authors would like to thank Arlene Oatway for her help with the microscopy work carried out in this study, as well as Annie Wong for her technical assistance throughout. RJW acknowledges the support provided by the Alberta Innovates Bio Solutions, Alberta Enterprise and Advance Education, the Canada Foundation for Innovation, the Canada Research Chairs Program and the Natural Sciences and Engineering Research Council of Canada. NR 58 TC 3 Z9 3 U1 10 U2 10 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD AUG PY 2016 VL 67 IS 15 BP 4627 EP 4638 DI 10.1093/jxb/erw242 PG 12 WC Plant Sciences SC Plant Sciences GA DV9BD UT WOS:000383233400017 PM 27325892 ER PT J AU Slattery, RA Grennan, AK Sivaguru, M Sozzani, R Ort, DR AF Slattery, Rebecca A. Grennan, Aleel K. Sivaguru, Mayandi Sozzani, Rosangela Ort, Donald R. TI Light sheet microscopy reveals more gradual light attenuation in light-green versus dark-green soybean leaves SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Chlorophyll; Glycine max; leaf light environment; light sheet microscopy; light use efficiency; photosynthesis; photosynthetic efficiency; soybean ID PALISADE TISSUE CHLOROPLASTS; CHLOROPHYLL FLUORESCENCE; PHOTOSYNTHETIC PROPERTIES; SPINACH LEAVES; LEAF PHOTOSYNTHESIS; ANTENNA SIZE; ABSORPTION; ENVIRONMENT; PROFILES; FIXATION AB Light sheet microscopy, a novel approach to quantifying light profiles, showed more gradual light attenuation in light-green soybean leaves compared to dark-green soybean.Light wavelengths preferentially absorbed by chlorophyll (chl) often display steep absorption gradients. This over-saturates photosynthesis in upper chloroplasts and deprives lower chloroplasts of blue and red light. Reducing chl content could create a more even leaf light distribution and thereby increase leaf light-use efficiency and overall canopy photosynthesis. This was tested on soybean cultivar 'Clark' (WT) and a near-isogenic chl b deficient mutant, 11y11, grown in controlled environment chambers and in the field. Light attenuation was quantified using a novel approach involving light sheet microscopy. Leaf adaxial and abaxial surfaces were illuminated separately with blue, red, and green wavelengths, and chl fluorescence was detected orthogonally to the illumination plane. Relative fluorescence was significantly greater in deeper layers of the Y11y11 mesophyll than in WT, with the greatest differences in blue, then red, and finally green light when illuminated from the adaxial surface. Modeled relative photosynthesis based on chlorophyll profiles and Beer's Law predicted less steep gradients in mutant relative photosynthesis rates compared to WT. Although photosynthetic light-use efficiency was greater in the field-grown mutant with similar to 50% lower chl, light-use efficiency was lower in the mutant when grown in chambers where chl was similar to 80% reduced. This difference is probably due to pleiotropic effects of the mutation that accompany very severe reductions in chlorophyll and may warrant further testing in other low-chl lines. C1 [Slattery, Rebecca A.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, 1206 West Gregory Dr, Urbana, IL 61801 USA. [Slattery, Rebecca A.; Grennan, Aleel K.; Sivaguru, Mayandi; Ort, Donald R.] Univ Illinois, Carl R Woese Inst Genom Biol, 1206 West Gregory Dr, Urbana, IL 61801 USA. [Sozzani, Rosangela] North Carolina State Univ, Dept Plant & Microbial Biol, 2115 Gardner Hall,Box 7612, Raleigh, NC 27695 USA. [Ort, Donald R.] USDA, Global Change & Photosynth Res Unit, 1206 West Gregory Dr, Urbana, IL 61801 USA. RP Ort, DR (reprint author), Univ Illinois, Dept Plant Biol, 1206 West Gregory Dr, Urbana, IL 61801 USA.; Ort, DR (reprint author), Univ Illinois, Carl R Woese Inst Genom Biol, 1206 West Gregory Dr, Urbana, IL 61801 USA.; Ort, DR (reprint author), USDA, Global Change & Photosynth Res Unit, 1206 West Gregory Dr, Urbana, IL 61801 USA. EM d-ort@illinois.edu NR 43 TC 1 Z9 1 U1 13 U2 13 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD AUG PY 2016 VL 67 IS 15 BP 4697 EP 4709 DI 10.1093/jxb/erw246 PG 13 WC Plant Sciences SC Plant Sciences GA DV9BD UT WOS:000383233400023 PM 27329746 ER PT J AU Dashti, HS Zuurbier, LA de Jonge, E Voortman, T Jacques, PF Lamon-Fava, S Scheer, FAJL Kiefte-De Jong, JC Hofman, A Ordovas, JM Franco, OH Tiemeier, H AF Dashti, Hassan S. Zuurbier, Lisette A. de Jonge, Ester Voortman, Trudy Jacques, Paul F. Lamon-Fava, Stefania Scheer, Frank A. J. L. Kiefte-De Jong, Jessica C. Hofman, Albert Ordovas, Jose M. Franco, Oscar H. Tiemeier, Henning TI Actigraphic sleep fragmentation, efficiency and duration associate with dietary intake in the Rotterdam Study SO JOURNAL OF SLEEP RESEARCH LA English DT Article DE dietary intake; epidemiology; macronutrients; sleep duration ID FOOD-INTAKE; OBESITY; METAANALYSIS; QUALITY; ADULTS; WOMEN; INDEX; INTERVENTION; INDIVIDUALS; CONSUMPTION AB Short self-reported sleep duration is associated with dietary intake and this association may partly mediate the link between short sleep and metabolic abnormalities. Subjective sleep measures, however, may be inaccurate and biased. The objective of this study was to evaluate the associations between actigraphic measures of sleep fragmentation, efficiency and duration and energy and macronutrient intakes. We used data from a subgroup of 439 participants of the population-based cohort, Rotterdam Study. Sleep was assessed using 7-day actigraphy and sleep diaries, and dietary data with a validated food frequency questionnaire. We assessed the associations of actigraphic sleep parameters with dietary intake using multivariable linear regression models. Higher sleep fragmentation was associated with 4.19 g lower carbohydrate intake per standard deviation of fragmentation {beta [95% confidence interval (CI) = -4.19 (-8.0, -0.3)]; P = 0.03}. Each additional percentage increase in sleep efficiency was associated with 11.1 kcal lower energy intake [beta (95% CI) = -11.1 (-20.6, -1.7); P = 0.02]. Furthermore, very short sleep duration (<5.5 h) was associated with 218.1 kcal higher energy intake [b (95% CI = 218.06 (33.3, 402.8), P = 0.02], relative to the reference group (>= 6.5 to <7.5 h). We observed associations between higher sleep fragmentation with lower carbohydrate intake, and both lower sleep efficiency and very short sleep duration (<5 h) with higher energy intake. The association between sleep and higher energy intake could mediate, in part, the link between short sleep or sleep fragmentation index and metabolic abnormalities. C1 [Dashti, Hassan S.; Ordovas, Jose M.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Dashti, Hassan S.; Zuurbier, Lisette A.; de Jonge, Ester; Voortman, Trudy; Kiefte-De Jong, Jessica C.; Hofman, Albert; Franco, Oscar H.; Tiemeier, Henning] Univ Med Ctr, Erasmus MC, Dept Epidemiol, POB 2040, NL-3000 CA Rotterdam, Netherlands. [de Jonge, Ester] Univ Med Ctr, Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Jacques, Paul F.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Nutr Epidemiol Lab, Boston, MA 02111 USA. [Lamon-Fava, Stefania] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Scheer, Frank A. J. L.] Brigham & Womens Hosp, Div Sleep & Circadian Disorders, 75 Francis St, Boston, MA 02115 USA. [Scheer, Frank A. J. L.] Harvard Med Sch, Div Sleep Med, Boston, MA USA. [Kiefte-De Jong, Jessica C.] Leiden Univ Coll, Global Publ Hlth, The Hague, Netherlands. [Ordovas, Jose M.] CNIC, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. [Tiemeier, Henning] Univ Med Ctr, Erasmus MC, Dept Psychiat, Rotterdam, Netherlands. RP Tiemeier, H (reprint author), Univ Med Ctr, Erasmus MC, Dept Epidemiol, POB 2040, NL-3000 CA Rotterdam, Netherlands. EM h.tiemeier@erasmusmc.nl OI Dashti, Hassan S/0000-0002-1650-679X FU Dr James Sadowski Memorial Internship; US Department of Agriculture [58-1950-0-014]; Erasmus-MC; Erasmus University, Rotterdam; Netherlands Organization for the Health Research and Development (ZonMw); Ministry of Education, Culture and Science; European Commission (DGXII); Netherlands Organization for Scientific Research [NWO-VIDI: 017.106.370]; Nestle Nutrition (Nestec Ltd); Metagenics Inc.; AXA; Dutch Organization for Scientific Research (NWO) [022.002.023]; NIH [R21 DK089378, R01 HL094806] FX This study was supported by the Dr James Sadowski Memorial Internship and the US Department of Agriculture, agreement no. 58-1950-0-014. The Rotterdam Study is funded by Erasmus-MC and Erasmus University, Rotterdam, the Netherlands Organization for the Health Research and Development (ZonMw), the Ministry of Education, Culture and Science and the European Commission (DGXII). Sleep research in the Rotterdam Study was supported by the Netherlands Organization for Scientific Research grant (NWO-VIDI: 017.106.370) awarded to Dr Tiemeier. EdJ, TV and OhF work in ErasmusAGE, a centre for ageing research funded by Nestle Nutrition (Nestec Ltd); Metagenics Inc.; and AXA. Nestle Nutrition, Metagenics Inc. and AXA had no role in the design or conduct of the study; collection, management, analysis or interpretation of the data; and preparation, review or approval of the manuscript. EdJ is supported by a grant from the Dutch Organization for Scientific Research (NWO) for the graduate programme of 2010 (project number: 022.002.023). FAJLS was supported in part by NIH grants R21 DK089378 and R01 HL094806. NR 43 TC 2 Z9 2 U1 2 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1105 EI 1365-2869 J9 J SLEEP RES JI J. Sleep Res. PD AUG PY 2016 VL 25 IS 4 BP 404 EP 411 DI 10.1111/jsr.12397 PG 8 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA DW2EI UT WOS:000383455300005 PM 26857552 ER PT J AU Powell, MR AF Powell, Mark R. TI Trends in Reported Foodborne Illness in the United States; 1996-2013 SO RISK ANALYSIS LA English DT Article DE Foodborne illness; retrospective analysis; trends ID SURVEILLANCE NETWORK FOODNET; INFECTIONS; PATHOGENS; CHILDREN AB Retrospective review is a key to designing effective food safety measures. The analysis examines trends in the reported incidence of illness due to bacterial pathogens commonly transmitted by food in the United States during 1996-2013 with and without specifying a model form for trend. The findings indicate early declines in reported incidence followed by a period of no significant trend for Campylobacter, Listeria, Shiga toxin-producing Escherichia coli O157, and Yersinia. The results are inconclusive about whether there is no trend or an increasing trend for Salmonella. While Shigella exhibits a continuous decline, Vibrio exhibits a continuous increase. Overall, the findings indicate a lack of evidence for continuous reduction in illness due to bacterial pathogens commonly transmitted by food in the United States during 1996-2013. C1 [Powell, Mark R.] USDA, 1400 Independence Ave SW MS 3811, Washington, DC 20250 USA. RP Powell, MR (reprint author), USDA, 1400 Independence Ave SW MS 3811, Washington, DC 20250 USA. EM mpowell@oce.usda.gov NR 17 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 EI 1539-6924 J9 RISK ANAL JI Risk Anal. PD AUG PY 2016 VL 36 IS 8 SI SI BP 1589 EP 1598 DI 10.1111/risa.12530 PG 10 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA DW6OU UT WOS:000383771600009 PM 26709453 ER PT J AU Bamberg, J del Rio, A Navarre, DA AF Bamberg, John del Rio, Alfonso Navarre, Duroy A. TI Intuitive Visual Impressions (Cogs) for Identifying Clusters of Diversity within Potato Species SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Intuitive clusters; Classification; Cog; Germplasm; Predictivity ID CULTIVATED POTATO; SOLANUM-TUBEROSUM; WILD; AFLP; GERMPLASM; CORE AB One of the basic research activities of genebanks is to partition stocks into groups that facilitate the efficient preservation and evaluation of the full range of useful phenotype diversity. We sought to test the usefulness of making intraspecific groups by replicated rapid visual intuitive impressions of coded plants by multiple uncoached observers. We invented the term "cog" (shorthand for cognate = "born together") to indicate assumed genetic relatedness of cog members. All of the 16 populations of the wild potato species Solanum okadae in the genebank were thus examined in four separate grow-outs by up to seven genebank staff members, a total of 26 times. They were instructed to place them into two cogs defined only as big and not-big. Four populations were placed in the big cog for 70-90 % of observations, while all remaining populations but one were placed in the big cog for less than 5 % of observations. All populations were then assessed for DNA markers and various empirical traits. AFLP and SNP markers clearly distinguished the two cogs. The big cog populations were also distinguished from the others by virtue of having less foliar late blight resistance, more leaf hairiness, and lower tuber tomatine content. SNP similarity suggests one population of reputed Bolivian origin is really a mislabeled duplicate of another from Argentina. If so, the two cogs also perfectly align with country of natural origin, with big originating exclusively from Bolivia. Using S. okadae as a model, we demonstrated that rapid, simple, and inexpensive visual intuitive cogs reliably predict significant genetic and phenotypic differences. We propose testing the cog technique on other species and applying it as a new potato germplasm descriptor. C1 [Bamberg, John; del Rio, Alfonso] USDA ARS, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. [Navarre, Duroy A.] USDA ARS, Prosser, WA 99350 USA. RP Bamberg, J (reprint author), USDA ARS, US Potato Genebank, 4312 Hwy 42, Sturgeon Bay, WI 54235 USA. EM John.Bamberg@ars.usda.gov OI Del Rio, Alfonso/0000-0001-8780-747X NR 32 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2016 VL 93 IS 4 BP 350 EP 359 DI 10.1007/s12230-016-9508-6 PG 10 WC Agronomy SC Agriculture GA DU3MO UT WOS:000382115400004 ER PT J AU Kittipadakul, P Jaipeng, B Slater, A Stevenson, W Jansky, S AF Kittipadakul, Piya Jaipeng, Boonsri Slater, Anthony Stevenson, Walter Jansky, Shelley TI Potato Production in Thailand SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Commercial production; Southeast Asia AB Potato production has increased dramatically in recent years in Thailand. Consumer demand for fresh and processed potatoes has driven this trend. Most potatoes are produced in northern Thailand in either double cropping highland zones or as a single winter crop following rice in lowland regions. Major production constraints are quality seed, cultivars adapted to short season warm climates, and high disease incidence. There is a need for increased research for cultivar development, access to high quality seed and improved commercial potato production practices. C1 [Kittipadakul, Piya] Kasetsart Univ, Dept Agron, Bangkok, Thailand. [Jaipeng, Boonsri] Potato Grower, Chiang Mai, Thailand. [Slater, Anthony] DEDJTR, AgriBio, Bundoora, Vic, Australia. [Stevenson, Walter] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Jansky, Shelley] USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Jansky, Shelley] Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. RP Jansky, S (reprint author), USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA.; Jansky, S (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM shelley.jansky@ars.usda.gov NR 7 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2016 VL 93 IS 4 BP 380 EP 385 DI 10.1007/s12230-016-9511-y PG 6 WC Agronomy SC Agriculture GA DU3MO UT WOS:000382115400008 ER PT J AU Cooper, WR Bamberg, JB AF Cooper, W. Rodney Bamberg, John B. TI Variation in Susceptibility to Potato Psyllid, Bactericera cockerelli (Hemiptera: Triozidae), among Solanum verrucosum Germplasm Accessions SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Potato psyllid; Tomato psyllid; Resistance; Liberibacter; Wild potato ID CANDIDATUS LIBERIBACTER SOLANACEARUM; ZEBRA CHIP DISEASE; RESISTANCE; SULC; WILD; SYSTEMATICS; MANAGEMENT; PCR AB Potato psyllid, Bactericera cockerelli (ulc) (Hemiptera: Triozidae), is a key pest of potato and the vector of "Candidatus Liberibacter solanacearum," the pathogen associated with zebra chip disease. Development of potato cultivars with genetic resistance to potato psyllid would enable cost-effective control of this pest with reduced use of insecticides. To facilitate the development of resistant cultivars, the objective of our study was to screen germplasm accessions of Solanum verrucosum for resistance to potato psyllid. The susceptibility of S. verrucosum germplasm accessions to potato psyllid was highly variable in choice prescreening assays and no-choice performance assays. Compared with the susceptible potato cultivar, 'Russet Burbank,' several S. verrucosum populations exhibited strong resistance to potato psyllid. The S. verrucosum accession, PI 195170 was highly resistant to potato psyllid, and is a potential source of genetic resistance for the development of resistant potato cultivars. C1 [Cooper, W. Rodney] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Bamberg, John B.] USDA ARS, US Potato Genebank, 6312 Hwy 42, Sturgeon Bay, WI 54235 USA. RP Cooper, WR (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Rodney.Cooper@ars.usda.gov RI Cooper, William/D-3205-2017 FU USDA-ARS Germplasm Crop Committee FX Pauline Anderson and Heather Headrick provided technical assistance. Partial funding was provided by the USDA-ARS Germplasm Crop Committee. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 24 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2016 VL 93 IS 4 BP 386 EP 391 DI 10.1007/s12230-016-9512-x PG 6 WC Agronomy SC Agriculture GA DU3MO UT WOS:000382115400009 ER PT J AU Wang, Y Tiwari, VK Rawat, N Gill, BS Huo, NX You, FM Coleman-Derr, D Gu, YQ AF Wang, Yi Tiwari, Vijay K. Rawat, Nidhi Gill, Bikram S. Huo, Naxin You, Frank M. Coleman-Derr, Devin Gu, Yong Q. TI GSP: a web-based platform for designing genome-specific primers in polyploids SO BIOINFORMATICS LA English DT Article ID HIGH-THROUGHPUT; PIPELINE AB Motivation: The sequences among subgenomes in a polyploid species have high similarity, making it difficult to design genome-specific primers for sequence analysis. Results: We present GSP, a web-based platform to design genome-specific primers that distinguish subgenome sequences in a polyploid genome. GSP uses BLAST to extract homeologous sequences of the subgenomes in existing databases, performs a multiple sequence alignment, and design primers based on sequence variants in the alignment. An interactive primers diagram, a sequence alignment viewer and a virtual electrophoresis are displayed as parts of the primer design result. GSP also designs specific primers from multiple sequences uploaded by users. Availability and implementation: GSP is a user-friendly and efficient web platform freely accessible at http://probes.pw.usda.gov/GSP. Source code and command-line application are available at https://github.com/bioinfogenome/GSP. C1 [Wang, Yi; Huo, Naxin; Gu, Yong Q.] USDA ARS, Western Reg Res Ctr, Crop Improvement & Genet Res Unit, 800 Buchanan St, Albany, CA 94710 USA. [Wang, Yi; Coleman-Derr, Devin] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Tiwari, Vijay K.; Rawat, Nidhi; Gill, Bikram S.] Kansas State Univ, Dept Plant Pathol, Wheat Genet Resource Ctr, Throckmorton Hall, Manhattan, KS 66506 USA. [You, Frank M.] Agr & Agri Food Canada, Cereal Res Ctr, Morden, MB R6M 1Y5, Canada. RP Gu, YQ (reprint author), USDA ARS, Western Reg Res Ctr, Crop Improvement & Genet Res Unit, 800 Buchanan St, Albany, CA 94710 USA.; Coleman-Derr, D (reprint author), USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. EM devin.coleman-derr@ars.usda.gov; yong.gu@ars.usda.gov FU U.S. National Science Foundation [IOS 0822100]; United State Department of Agriculture Research Service CRIS project [5325-21000-021]; NSF-IUCRC [IIP1338897] FX This work has been supported in part by U.S. National Science Foundation (Grant number IOS 0822100) and by United State Department of Agriculture Research Service CRIS project 5325-21000-021. VKT was supported by NSF-IUCRC grant contract (IIP1338897). NR 6 TC 0 Z9 0 U1 1 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1367-4803 EI 1460-2059 J9 BIOINFORMATICS JI Bioinformatics PD AUG 1 PY 2016 VL 32 IS 15 BP 2382 EP 2383 DI 10.1093/bioinformatics/btw134 PG 2 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Computer Science, Interdisciplinary Applications; Mathematical & Computational Biology; Statistics & Probability SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Computer Science; Mathematical & Computational Biology; Mathematics GA DV8JY UT WOS:000383184500025 PM 27153733 ER PT J AU Jackson, BR Tarr, C Strain, E Jackson, KA Conrad, A Carleton, H Katz, LS Stroika, S Gould, LH Mody, RK Silk, BJ Beal, J Chen, Y Timme, R Doyle, M Fields, A Wise, M Tillman, G Defibaugh-Chavez, S Kucerova, Z Sabol, A Roache, K Trees, E Simmons, M Wasilenko, J Kubota, K Pouseele, H Klimke, W Besser, J Brown, E Allard, M Gerner-Smidt, P AF Jackson, Brendan R. Tarr, Cheryl Strain, Errol Jackson, Kelly A. Conrad, Amanda Carleton, Heather Katz, Lee S. Stroika, Steven Gould, L. Hannah Mody, Rajal K. Silk, Benjamin J. Beal, Jennifer Chen, Yi Timme, Ruth Doyle, Matthew Fields, Angela Wise, Matthew Tillman, Glenn Defibaugh-Chavez, Stephanie Kucerova, Zuzana Sabol, Ashley Roache, Katie Trees, Eija Simmons, Mustafa Wasilenko, Jamie Kubota, Kristy Pouseele, Hannes Klimke, William Besser, John Brown, Eric Allard, Marc Gerner-Smidt, Peter TI Implementation of Nationwide Real-time Whole-genome Sequencing to Enhance Listeriosis Outbreak Detection and Investigation SO CLINICAL INFECTIOUS DISEASES LA English DT Article DE Listeria monocytogenes; DNA sequencing; outbreaks; foodborne diseases ID UNITED-STATES; FOODBORNE LISTERIOSIS; MONOCYTOGENES; SURVEILLANCE; PATHOGENS; STRAINS; CLONES; FOOD AB Listeria monocytogenes (Lm) causes severe foodborne illness (listeriosis). Previous molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE), were critical in detecting outbreaks that led to food safety improvements and declining incidence, but PFGE provides limited genetic resolution. A multiagency collaboration began performing real-time, whole-genome sequencing (WGS) on all US Lm isolates from patients, food, and the environment in September 2013, posting sequencing data into a public repository. Compared with the year before the project began, WGS, combined with epidemiologic and product trace-back data, detected more listeriosis clusters and solved more outbreaks (2 outbreaks in pre-WGS year, 5 in WGS year 1, and 9 in year 2). Whole-genome multilocus sequence typing and single nucleotide polymorphism analyses provided equivalent phylogenetic relationships relevant to investigations; results were most useful when interpreted in context of epidemiological data. WGS has transformed listeriosis outbreak surveillance and is being implemented for other foodborne pathogens. C1 [Jackson, Brendan R.; Tarr, Cheryl; Jackson, Kelly A.; Conrad, Amanda; Carleton, Heather; Katz, Lee S.; Stroika, Steven; Gould, L. Hannah; Mody, Rajal K.; Silk, Benjamin J.; Wise, Matthew; Kucerova, Zuzana; Sabol, Ashley; Roache, Katie; Trees, Eija; Besser, John; Gerner-Smidt, Peter] Ctr Dis Control & Prevent, 1600 Clifton Rd NE,MS C-09, Atlanta, GA 30329 USA. [Strain, Errol; Beal, Jennifer; Chen, Yi; Timme, Ruth; Doyle, Matthew; Fields, Angela; Brown, Eric; Allard, Marc] US FDA, College Pk, MD USA. [Tillman, Glenn; Simmons, Mustafa; Wasilenko, Jamie] US Food Safety & Inspect Serv, USDA, Athens, GA USA. [Defibaugh-Chavez, Stephanie] US Food Safety & Inspect Serv, USDA, Washington, DC 20250 USA. [Kubota, Kristy] Assoc Publ Hlth Labs, Silver Spring, MD USA. [Pouseele, Hannes] Appl Maths, Sint Martens Latem, Belgium. [Klimke, William] Natl Inst Biotechnol Informat, NIH, Bethesda, MD USA. RP Jackson, BR (reprint author), Ctr Dis Control & Prevent, 1600 Clifton Rd NE,MS C-09, Atlanta, GA 30329 USA. EM brjackson1@cdc.gov FU CDC's Advanced Molecular Detection Initiative; CDC [U60HM000803]; FDA; USDA-FSIS; National Institute for Biotechnology Information; NIH, National Library of Medicine FX This work was supported by CDC, including CDC's Advanced Molecular Detection Initiative and Cooperative Agreement number U60HM000803; FDA; USDA-FSIS; National Institute for Biotechnology Information; and the Intramural Research Program of the NIH, National Library of Medicine. H. P. is an employee of Applied Maths. NR 30 TC 13 Z9 13 U1 17 U2 17 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD AUG 1 PY 2016 VL 63 IS 3 BP 380 EP 386 DI 10.1093/cid/ciw242 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA DV8QP UT WOS:000383201900014 PM 27090985 ER PT J AU Davis, AJ Phillips, ML Doherty, PF AF Davis, Amy J. Phillips, Michael L. Doherty, Paul F., Jr. TI Declining recruitment of Gunnison Sage-Grouse highlights the need to monitor juvenile survival SO CONDOR LA English DT Article DE Centrocercus minimus; chick survival; Gunnison Sage-Grouse; juvenile recruitment; population trends ID CENTROCERCUS-UROPHASIANUS POPULATIONS; NORTH-AMERICA; CHICKS; MANAGEMENT; SELECTION; HABITAT; OREGON; RADIOTRANSMITTERS; MOVEMENTS; VARIANCE AB Recruitment of juveniles is an important vital rate that influences population growth and is fundamental to understanding trends in population size. Estimates of recruitment are often focused on the period just after hatching (prefledgling stage), which is typically the lowest survival period and often the most variable. Few studies examine true recruitment- survival from hatching to entering the breeding population-although this information is more relevant to understanding population trends. We studied the recruitment of Gunnison Sage-Grouse (Centrocercus minimus), a federally threatened species in the U.S., to examine the relative importance of chick and juvenile survival to recruitment patterns. We evaluated recruitment from 2005 to 2010 by combining separate estimates of chick survival (hatching to 30 days of age) and juvenile survival (31 days of age to the start of the first breeding season). To explain variation in these survival rates, we examined the effects of population, individual (i.e. age), and temporal (within-year and among-year differences) factors associated with recruitment of Gunnison Sage-Grouse. The factors that most explained juvenile survival rates were temporal (among-year trends and within-year seasonal effects). Chick survival rates varied by population, and daily chick survival increased with chick age. We found a slight negative trend in chick survival and a strong negative trend in juvenile survival from 2005 to 2010. The overall recruitment rate declined from 0.32 (+/- 0.09 SE) in 2005 to 0.04 (+/- 0.03 SE) in 2010. This decline coincided with a decline observed in population index data, which was not reflected in other demographic data. If survival had not been monitored past 30 days of age, estimates of recruitment would have remained relatively stable. This work highlights the importance of monitoring juvenile survival, as it may influence population dynamics. C1 [Davis, Amy J.; Doherty, Paul F., Jr.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Phillips, Michael L.] Colorado Pk & Wildlife, Ft Collins, CO USA. [Davis, Amy J.] Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO 80526 USA. RP Davis, AJ (reprint author), Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA.; Davis, AJ (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO 80526 USA. EM Amy.J.Davis@aphis.usda.gov FU Colorado Parks and Wildlife; National Parks Service; U.S. Geological Survey; U.S. Fish and Wildlife Service; Colorado State University FX Funding statement: Funding for this project was provided by Colorado Parks and Wildlife, the National Parks Service, the U.S. Geological Survey, the U.S. Fish and Wildlife Service, and Colorado State University. None of the funders had any input into the content of the manuscript, nor required their approval of the manuscript prior to submission or publication. NR 61 TC 0 Z9 0 U1 5 U2 5 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD AUG PY 2016 VL 118 IS 3 BP 477 EP 488 DI 10.1650/CONDOR-15-165.1 PG 12 WC Ornithology SC Zoology GA DV7BN UT WOS:000383091000003 ER PT J AU Jenkins, JMA Thompson, FR Faaborg, J AF Jenkins, Julianna M. A. Thompson, Frank R., III Faaborg, John TI Contrasting patterns of nest survival and postfledging survival in Ovenbirds and Acadian Flycatchers in Missouri forest fragments SO CONDOR LA English DT Article DE postfledging survival; nest success; population growth; Seiurus aurocapilla; radio-telemetry; Empidonax virescens ID SEIURUS-AUROCAPILLA; JUVENILE SURVIVAL; HABITAT SELECTION; MIGRATORY BIRDS; MIGRANT BIRDS; POPULATION; LANDSCAPE; SUCCESS; CONSERVATION; PRODUCTIVITY AB We can improve our ability to assess population viability and forecast population growth under different scenarios by understanding factors that limit population parameters in each stage of the annual cycle. Postfledging mortality rates may be as variable as nest survival across regions and fragmentation gradients, although factors that negatively impact nest survival may affect postfledging individuals in different ways. We examined nest and postfledging survival of Ovenbirds (Seiurus aurocapilla) and Acadian Flycatchers (Empidonax virescens) in mature forest fragments in central Missouri. We used an information-theoretic approach to determine support for effects of factors intrinsic to the individual or nest site, temporal factors, local vegetation characteristics, and distance to edge on survival in both stages. We also examined the effect of incorporating the resulting survival estimates on population growth. In both species, survival increased from nest to postfledging stages (Ovenbirds: 0.27 +/- 0.06 to 0.50 +/- 0.09; Acadian Flycatcher 0.30 +/- 0.03 to 0.89 +/- 0.11). Age was by far the best predictor of survival in postfledging birds, with the majority of mortalities occurring in the first week out of the nest. We did not find support for survival tradeoffs of habitat used by nesting or postfledging birds. Acadian Flycatcher nest and postfledging survival were both related to variables associated with mature forest. Ovenbird nest survival was most affected by habitat characteristics associated with core mature forest, although postfledging survival may have improved near non-forest edges. We replaced an arbitrary estimate of juvenile survival (half of adult survival) with an estimate incorporating empirical postfledging survival estimates. With these revised parameters, Acadian Flycatcher population growth was more affected (13-26% increase in lambda) than Ovenbird population growth (3-6% change). Our results illustrate that species occupying similar nesting habitat do not necessarily face the same risks during the postfledging period. C1 [Jenkins, Julianna M. A.; Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] US Forest Serv, Northern Res Stn, Columbia, MO USA. RP Jenkins, JMA (reprint author), Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. EM jmappd@mail.missouri.edu FU USDA Forest Service Northern Research Station; Audubon Society of Missouri Graduate Research Scholarship; University of Missouri's Conservation Biology Scholarship FX Major funding for this project was provided by the USDA Forest Service Northern Research Station; the Forest Service had no input to the content of the paper but reviewed and approved the manuscript prior to publication. Additional support came from the Audubon Society of Missouri Graduate Research Scholarship, and the University of Missouri's Conservation Biology Scholarship; these funders had no input to the paper and did not require their approval of the manuscript before submission or publication. NR 64 TC 1 Z9 1 U1 5 U2 5 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD AUG PY 2016 VL 118 IS 3 BP 583 EP 596 DI 10.1650/CONDOR-16-30.1 PG 14 WC Ornithology SC Zoology GA DV7BN UT WOS:000383091000011 ER PT J AU Bettazzi, F Martellini, T Shelver, WL Cincinelli, A Lanciotti, E Palchetti, I AF Bettazzi, Francesca Martellini, Tania Shelver, Weilin L. Cincinelli, Alessandra Lanciotti, Eudes Palchetti, Ilaria TI Development of an Electrochemical Immunoassay for the Detection of Polybrominated Diphenyl Ethers (PBDEs) SO ELECTROANALYSIS LA English DT Article DE electrochemical immunoassay; PBDEs; magnetic beads; screen-printed electrodes; GC-MS ID MAGNETO-IMMUNOSENSOR; FLAME RETARDANTS; SAMPLES; BIOASSAY; HEALTH; WASTE; ELISA; WATER AB Polybrominated diphenyl ethers (PBDEs) are persistent environmental substances that were commonly used as fire retardants in a wide number of commercial products. Their low reactivity, high hydrophobicity and bioaccumulative properties cause their ubiquity in the air, water, food and lead to extensive exposure of world population to these compounds. The severe health problems caused by PBDEs lead them to be banned from the market. In March 2014 the European Commission issued a recommendation in which member states are requested to monitor brominated flame retardants in food, in order to evaluate human and wildlife exposure. Here, we described the development of an electrochemical magnetic particle enzyme-linked immunoassay to analyze PBDEs in food samples. The immunological reaction is based on a competitive scheme, using an alkaline phosphatase labeled congener as tracer. The anti-PBDE antibody modified magnetic particles are captured on the surface of carbon disposable array of sensors. The reaction extent is finally electrochemically measured by differential pulse voltammetry, upon the addition of substrate. Under the optimized conditions, a limit of detection of 0.18ng/mL with a limit of quantification of 0.30ng/mL and a quantification range of 0.30-6.9ng/mL, (RSD%=12) is obtained. Results of food samples obtained from the newly developed electrochemical immunoassay are also reported. C1 [Bettazzi, Francesca; Martellini, Tania; Cincinelli, Alessandra; Palchetti, Ilaria] Univ Florence, Dipartimento Chim, Via Lastruccia 3, I-50019 Florence, Italy. [Shelver, Weilin L.] ARS, USDA, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. [Lanciotti, Eudes] Univ Florence, DSS, Viale Morgagni 48, I-850134 Florence, Italy. RP Palchetti, I (reprint author), Univ Florence, Dipartimento Chim, Via Lastruccia 3, I-50019 Florence, Italy. EM ilaria.palchetti@unifi.it OI palchetti, ilaria/0000-0001-9366-0574 FU Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) [20128ZZS2H]; Ente Cassa di Risparmio [PED 8780 2014.0757A2202.0734] FX This work was supported by Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) in the framework of PRIN 2012 (grant no. 20128ZZS2H) and Ente Cassa di Risparmio project ID PED 8780 2014.0757A2202.0734. NR 28 TC 0 Z9 0 U1 13 U2 13 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA POSTFACH 101161, 69451 WEINHEIM, GERMANY SN 1040-0397 EI 1521-4109 J9 ELECTROANAL JI Electroanalysis PD AUG PY 2016 VL 28 IS 8 SI SI BP 1817 EP 1823 DI 10.1002/elan.201600127 PG 7 WC Chemistry, Analytical; Electrochemistry SC Chemistry; Electrochemistry GA DV0AI UT WOS:000382579300016 ER PT J AU Kurtzman, CP Robnett, CJ Blackwell, M AF Kurtzman, Cletus P. Robnett, Christie J. Blackwell, Meredith TI Description of Teunomyces gen. nov for the Candida kruisii clade, Suhomyces gen. nov for the Candida tanzawaensis clade and Suhomyces kilbournensis sp nov. SO FEMS YEAST RESEARCH LA English DT Article DE Candida; Suhomyces; Teunomyces; new genera; new species; yeasts ID BASIDIOMYCETOUS YEASTS; ASCOMYCETOUS YEASTS; FEEDING BEETLES; BIODIVERSITY; SYSTEMATICS; GENERA; D1/D2 AB DNA sequence analysis has shown that species of the Candida kruisii clade and species of the C. tanzawaensis clade represent phylogenetically circumscribed genera, which are described as Teunomyces gen. nov., type species T. kruisii, and Suhomyces gen. nov., type species S. tanzawaensis. Many of the species are distributed worldwide and they are often isolated from fungus-feeding insects and their habitats. Included is the description of S. kilbournensis (type strain NRRL Y-17864, CBS 14276), a species found almost exclusively on maize kernels (Zea mays) in IL, USA. C1 [Kurtzman, Cletus P.; Robnett, Christie J.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. [Blackwell, Meredith] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Blackwell, Meredith] Univ South Carolina, Dept Biol Sci, Columbia, SC 29208 USA. RP Kurtzman, CP (reprint author), ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 North Univ St, Peoria, IL 61604 USA. EM cletus.kurtzman@ars.usda.gov FU National Science Foundation [DEB-0072741, DEB-0417180]; Louisiana State University Boyd Professor Fund FX Much of the fieldwork and characterization of many of the species discussed was supported by the National Science Foundation (DEB-0072741 and DEB-0417180) and the Louisiana State University Boyd Professor Fund to MB. NR 28 TC 0 Z9 0 U1 2 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1567-1356 EI 1567-1364 J9 FEMS YEAST RES JI FEMS Yeast Res. PD AUG PY 2016 VL 16 IS 5 AR fow041 DI 10.1093/femsyr/fow041 PG 9 WC Biotechnology & Applied Microbiology; Microbiology; Mycology SC Biotechnology & Applied Microbiology; Microbiology; Mycology GA DV9AG UT WOS:000383231100013 ER PT J AU Barragan, R Coltell, O Asensio, EM Frances, F Sorli, JV Estruch, R Salas-Huetos, A Ordovas, JM Corella, D AF Barragan, Rocio Coltell, Oscar Asensio, Eva M. Frances, Francesc Sorli, Jose V. Estruch, Ramon Salas-Huetos, Albert Ordovas, Jose M. Corella, Dolores TI MicroRNAs and Drinking: Association between the Pre-miR-27a rs895819 Polymorphism and Alcohol Consumption in a Mediterranean Population SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE microRNAs; alcohol; miR27a; Mediterranean ID SINGLE NUCLEOTIDE POLYMORPHISMS; GASTRIC-CANCER SUSCEPTIBILITY; FUNCTIONAL POLYMORPHISM; MENDELIAN RANDOMIZATION; CHINESE POPULATION; COLORECTAL-CANCER; GENETIC VARIANT; HEART-DISEASE; USE DISORDERS; RISK AB Recently, microRNAs (miRNA) have been proposed as regulators in the different processes involved in alcohol intake, and differences have been found in the miRNA expression profile in alcoholics. However, no study has focused on analyzing polymorphisms in genes encoding miRNAs and daily alcohol consumption at the population level. Our aim was to investigate the association between a functional polymorphism in the pre-miR-27a (rs895819 A>G) gene and alcohol consumption in an elderly population. We undertook a cross-sectional study of PREvencion con DIeta MEDiterranea (PREDIMED)-Valencia participants (n = 1007, including men and women aged 67 +/- 7 years) and measured their alcohol consumption (total and alcoholic beverages) through a validated questionnaire. We found a strong association between the pre-miR-27a polymorphism and total alcohol intake, this being higher in GG subjects (5.2 +/- 0.4 in AA, 5.9 +/- 0.5 in AG and 9.1 +/- 1.8 g/day in GG; p(adjusted) = 0.019). We also found a statistically-significant association of the pre-miR-27a polymorphism with the risk of having a high alcohol intake (> 2 drinks/day in men and > 1 in women): 5.9% in AA versus 17.5% in GG; p(adjusted) < 0.001. In the sensitivity analysis, this association was homogeneous for sex, obesity and Mediterranean diet adherence. In conclusion, we report for the first time a significant association between a miRNA polymorphism (rs895819) and daily alcohol consumption. C1 [Barragan, Rocio; Asensio, Eva M.; Frances, Francesc; Sorli, Jose V.; Corella, Dolores] Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia 46010, Spain. [Barragan, Rocio; Coltell, Oscar; Asensio, Eva M.; Frances, Francesc; Sorli, Jose V.; Estruch, Ramon; Salas-Huetos, Albert; Corella, Dolores] Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid 28029, Spain. [Coltell, Oscar] Univ Jaume 1, Sch Technol & Expt Sci, Dept Comp Languages & Syst, Castellon de La Plana 12071, Spain. [Estruch, Ramon] Hosp Clin Barcelona, IDIBAPS, Dept Internal Med, Barcelona 08036, Spain. [Salas-Huetos, Albert] Univ Rovira & Virgili, IISPV, Dept Biochem & Biotechnol, Human Nutr Unit, Reus 43003, Spain. [Ordovas, Jose M.] Ctr Nacl Invest Cardiovasc CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid 28029, Spain. [Ordovas, Jose M.] IMDEA Alimentac, Madrid 28049, Spain. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Corella, D (reprint author), Univ Valencia, Sch Med, Dept Prevent Med & Publ Hlth, Valencia 46010, Spain.; Corella, D (reprint author), Inst Salud Carlos III, CIBER Fisiopatol Obesidad & Nutr, Madrid 28029, Spain. EM rocio.barragan@uv.es; oscar.coltell@uji.es; eva.m.asensio@uv.es; francesc.frances@uv.es; jose.sorli@uv.es; RESTRUCH@clinic.cat; albert.salas@uab.cat; jose.ordovas@tufts.edu; dolores.corella@uv.es RI Salas-Huetos, Albert/A-8509-2011; OI Salas-Huetos, Albert/0000-0001-5914-6862; Oscar, Coltell/0000-0002-4518-8495; BARRAGAN-ARNAL, ROCIO/0000-0003-0917-7251; Corella, Dolores/0000-0002-2366-4104 FU Spanish Ministry of Health (Instituto de Salud Carlos III); Ministerio de Economia y Competitividad-Fondo Europeo de Desarrollo Regional [CNIC-06/2007, RTIC G03/140, CIBER 06/03, PI06-1326, PI07-0954, PI11/02505, SAF2009-12304, AGL2010-22319-C03-03, PRX14/00527]; lUniversity Jaume I [P1-1B2013-54, 53-K06-5-10, 58-1950-9-001]; U.S. Department of Agriculture Research Service, USA; Generalitat Valenciana [ACOMP2010-181, AP111/10, AP-042/11, ACOM2011/145, ACOMP/2012/190, ACOMP/2013/159, ACOMP/213/165]; Colegio Complutense at Harvard University, Cambridge. MA, USA; Conselleria d'Educacio, Investigacio, Cultura i Esport. Generalitat Valenciana, Spain [ACIF/2013/168] FX This study was funded, by the Spanish Ministry of Health (Instituto de Salud Carlos III) and the Ministerio de Economia y Competitividad-Fondo Europeo de Desarrollo Regional (Projects CNIC-06/2007, RTIC G03/140, CIBER 06/03, PI06-1326, PI07-0954, PI11/02505, SAF2009-12304, AGL2010-22319-C03-03 and PRX14/00527), by the lUniversity Jaume I (Project P1-1B2013-54), by Contracts 53-K06-5-10 and 58-1950-9-001 from the U.S. Department of Agriculture Research Service, USA, by the Generalitat Valenciana (ACOMP2010-181, AP111/10, AP-042/11, ACOM2011/145, ACOMP/2012/190, ACOMP/2013/159 and ACOMP/213/165), and with the collaboration of the Real Colegio Complutense at Harvard University, Cambridge. MA, USA. Rocio Barragon's contract is funded by the Ayudas para la contratacion de personal investigador en formacion de caracter predoctoral, Programa "VALencia Investigacion mas Desarrollo" (VALi+d). Conselleria d'Educacio, Investigacio, Cultura i Esport. Generalitat Valenciana, Spain (ACIF/2013/168). NR 56 TC 0 Z9 0 U1 4 U2 4 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD AUG PY 2016 VL 17 IS 8 AR 1338 DI 10.3390/ijms17081338 PG 18 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DU6PU UT WOS:000382337900150 ER PT J AU Chen, MS Liu, SZ Wang, HY Cheng, XY El Bouhssini, M Whitworth, RJ AF Chen, Ming-Shun Liu, Sanzhen Wang, Haiyan Cheng, Xiaoyan El Bouhssini, Mustapha Whitworth, R. Jeff TI Genes Expressed Differentially in Hessian Fly Larvae Feeding in Resistant and Susceptible Plants SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE plant resistance; effectors; compatible interaction; incompatible interaction; salivary glands; cytochrome P450; RNA-sequencing; gall midge ID MAYETIOLA-DESTRUCTOR LARVAE; DIPTERA-CECIDOMYIIDAE; INCOMPATIBLE INTERACTIONS; SALIVARY-GLANDS; WINTER-WHEAT; RESPONSES; PROTEINS; GENETICS; METABOLISM; VIRULENT AB The Hessian fly, Mayetiola destructor, is a destructive pest of wheat worldwide and mainly controlled by deploying resistant cultivars. In this study, we investigated the genes that were expressed differentially between larvae in resistant plants and those in susceptible plants through RNA sequencing on the Illumina platform. Informative genes were 11,832, 14,861, 15,708, and 15,071 for the comparisons between larvae in resistant versus susceptible plants for 0.5, 1, 3, and 5 days, respectively, after larvae had reached the feeding site. The transcript abundance corresponding to 5401, 6902, 8457, and 5202 of the informative genes exhibited significant differences (p <= 0.05) in the respective paired comparisons. Overall, genes involved in nutrient metabolism, RNA and protein synthesis exhibited lower transcript abundance in larvae from resistant plants, indicating that resistant plants inhibited nutrient metabolism and protein production in larvae. Interestingly, the numbers of cytochrome P450 genes with higher transcript abundance in larvae from resistant plants were comparable to, or higher than those with lower transcript abundance, indicating that toxic chemicals from resistant plants may have played important roles in Hessian fly larval death. Our study also identified several families of genes encoding secreted salivary gland proteins (SSGPs) that were expressed at early stage of 1st instar larvae and with more genes with higher transcript abundance in larvae from resistant plants. Those SSGPs are candidate effectors with important roles in plant manipulation. C1 [Chen, Ming-Shun] Kansas State Univ, Hard Winter Wheat Genet Res Unit, USDA, ARS, 4008 Throckmorton, Manhattan, KS 66506 USA. [Chen, Ming-Shun; Cheng, Xiaoyan; Whitworth, R. Jeff] Kansas State Univ, Dept Entomol, 4008 Throckmorton, Manhattan, KS 66506 USA. [Liu, Sanzhen] Kansas State Univ, Dept Plant Pathol, 4008 Throckmorton, Manhattan, KS 66506 USA. [Wang, Haiyan] Kansas State Univ, Dept Stat, 4008 Throckmorton, Manhattan, KS 66506 USA. [El Bouhssini, Mustapha] Int Ctr Agr Res Dry Area, Rabat 10106, Morocco. RP Chen, MS (reprint author), Kansas State Univ, Hard Winter Wheat Genet Res Unit, USDA, ARS, 4008 Throckmorton, Manhattan, KS 66506 USA.; Chen, MS (reprint author), Kansas State Univ, Dept Entomol, 4008 Throckmorton, Manhattan, KS 66506 USA. EM mchen@ksu.edu; liu3zhen@ksu.edu; hwang@ksu.edu; xycheng@ksu.edu; M.BOHSSINI@cgiar.org; jwhitwor@k-state.edu FU United States Department of Agriculture-Agriculture Research Service at Manhattan, Kansas; Kansas Agricultural Experiment Station; U.S. Department of Agriculture (USDA NIFA) [2010-03741] FX This paper is a joint contribution from the United States Department of Agriculture-Agriculture Research Service at Manhattan, Kansas, and the Kansas Agricultural Experiment Station. Mention of a commercial or proprietary product does not constitute an endorsement or recommendation for its use by the USDA. USDA is an equal opportunity provider and employer. The research was partially supported by a grant from the U.S. Department of Agriculture (USDA NIFA 2010-03741). NR 43 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD AUG PY 2016 VL 17 IS 8 AR 1324 DI 10.3390/ijms17081324 PG 14 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DU6PU UT WOS:000382337900136 ER PT J AU Kogut, MH Swaggerty, CL Byrd, JA Selvaraj, R Arsenault, RJ AF Kogut, Michael H. Swaggerty, Christina L. Byrd, James Allen Selvaraj, Ramesh Arsenault, Ryan J. TI Chicken-Specific Kinome Array Reveals that Salmonella enterica Serovar Enteritidis Modulates Host Immune Signaling Pathways in the Cecum to Establish a Persistence Infection SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Article DE Salmonella; kinome; interferon-gamma; phospholipase c; JAK-STAT pathway ID REGULATORY T-CELLS; NF-KAPPA-B; TYPHIMURIUM INFECTION; DISEASE TOLERANCE; INNATE IMMUNITY; CARRIER-STATE; EXPRESSION; SUSCEPTIBILITY; PATHOGENESIS; VIRULENCE AB Non-typhoidal Salmonella enterica induces an early, short-lived pro-inflammatory response in chickens that is asymptomatic of clinical disease and results in a persistent colonization of the gastrointestinal (GI) tract that transmits infections to naive hosts via fecal shedding of bacteria. The underlying mechanisms that control this persistent colonization of the ceca of chickens by Salmonella are only beginning to be elucidated. We hypothesize that alteration of host signaling pathways mediate the induction of a tolerance response. Using chicken-specific kinomic immune peptide arrays and quantitative RT-PCR of infected cecal tissue, we have previously evaluated the development of disease tolerance in chickens infected with Salmonella enterica serovar Enteritidis (S. Enteritidis) in a persistent infection model (4-14 days post infection). Here, we have further outlined the induction of an tolerance defense strategy in the cecum of chickens infected with S. Enteritidis beginning around four days post-primary infection. The response is characterized by alterations in the activation of T cell signaling mediated by the dephosphorylation of phospholipase c-gamma 1 (PLCG1) that inhibits NF-kappa B signaling and activates nuclear factor of activated T-cells (NFAT) signaling and blockage of interferon-gamma (IFN-gamma) production through the disruption of the JAK-STAT signaling pathway (dephosphorylation of JAK2, JAK3, and STAT4). Further, we measured a significant down-regulation reduction in IFN-gamma mRNA expression. These studies, combined with our previous findings, describe global phenotypic changes in the avian cecum of Salmonella Enteritidis-infected chickens that decreases the host responsiveness resulting in the establishment of persistent colonization. The identified tissue protein kinases also represent potential targets for future antimicrobial compounds for decreasing Salmonella loads in the intestines of food animals before going to market. C1 [Kogut, Michael H.; Swaggerty, Christina L.; Byrd, James Allen] ARS, Southern Plains Agr Resarch Ctr, USDA, College Stn, TX 77845 USA. [Selvaraj, Ramesh] Ohio State Univ, Ohio Agr Res Ctr, Wooster, OH 44691 USA. [Arsenault, Ryan J.] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. RP Arsenault, RJ (reprint author), Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. EM mike.kogut@ars.usda.gov; christi.swaggerty@ars.usda.gov; allen.byrd@ars.usda.gov; selvaraj.7@osu.edu; rja@udel.edu FU USDA-ARS FX This study was supported by USDA-ARS intramural funding. NR 88 TC 1 Z9 1 U1 8 U2 8 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD AUG PY 2016 VL 17 IS 8 AR UNSP 1207 DI 10.3390/ijms17081207 PG 20 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DU6PU UT WOS:000382337900021 ER PT J AU Ye, ZJ Sangireddy, S Okekeogbu, I Zhou, SP Yu, CL Hui, DF Howe, KJ Fish, T Thannhauser, TW AF Ye, Zhujia Sangireddy, Sasikiran Okekeogbu, Ikenna Zhou, Suping Yu, Chih-Li Hui, Dafeng Howe, Kevin J. Fish, Tara Thannhauser, Theodore W. TI Drought-Induced Leaf Proteome Changes in Switchgrass Seedlings SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES LA English DT Review DE physiological properties; isobaric tags for relative and absolute quantitation (iTRAQ); ProteoMiner; functional pathways; abscisic acid (ABA) signaling; "Sandwich" plant growth system ID RAFFINOSE FAMILY OLIGOSACCHARIDES; GUANINE-NUCLEOTIDE EXCHANGE; ABSCISIC-ACID BIOSYNTHESIS; LOW ABUNDANCE PROTEINS; STRESS TOLERANCE; WATER-DEFICIT; RICE LEAVES; ARABIDOPSIS-THALIANA; CYTOSOLIC CALCIUM; SEED-GERMINATION AB Switchgrass (Panicum virgatum) is a perennial crop producing deep roots and thus highly tolerant to soil water deficit conditions. However, seedling establishment in the field is very susceptible to prolonged and periodic drought stress. In this study, a "sandwich" system simulating a gradual water deletion process was developed. Switchgrass seedlings were subjected to a 20-day gradual drought treatment process when soil water tension was increased to 0.05 MPa (moderate drought stress) and leaf physiological properties had expressed significant alteration. Drought-induced changes in leaf proteomes were identified using the isobaric tags for relative and absolute quantitation (iTRAQ) labeling method followed by nano-scale liquid chromatography mass spectrometry (nano-LC-MS/MS) analysis. Additionally, total leaf proteins were processed using a combinatorial library of peptide ligands to enrich for lower abundance proteins. Both total proteins and those enriched samples were analyzed to increase the coverage of the quantitative proteomics analysis. A total of 7006 leaf proteins were identified, and 257 (4% of the leaf proteome) expressed a significant difference (p < 0.05, fold change < 0.6 or > 1.7) from the non-treated control to drought-treated conditions. These proteins are involved in the regulation of transcription and translation, cell division, cell wall modification, phyto-hormone metabolism and signaling transduction pathways, and metabolic pathways of carbohydrates, amino acids, and fatty acids. A scheme of abscisic acid (ABA)-biosynthesis and ABA responsive signal transduction pathway was reconstructed using these drought-induced significant proteins, showing systemic regulation at protein level to deploy the respective mechanism. Results from this study, in addition to revealing molecular responses to drought stress, provide a large number of proteins (candidate genes) that can be employed to improve switchgrass seedling growth and establishment under soil drought conditions (Data are available via ProteomeXchange with identifier PXD004675). C1 [Ye, Zhujia; Sangireddy, Sasikiran; Okekeogbu, Ikenna; Zhou, Suping] Tennessee State Univ, Dept Agr Sci, 3500 John Merritt Blvd, Nashville, TN 37209 USA. [Yu, Chih-Li; Hui, Dafeng] Tennessee State Univ, Dept Biol Sci, 3500 John Merritt Blvd, Nashville, TN 37209 USA. [Howe, Kevin J.; Fish, Tara; Thannhauser, Theodore W.] Cornell Univ, Funct & Comparat Prote Ctr, USDA ARS, Ithaca, NY 14853 USA. RP Zhou, SP (reprint author), Tennessee State Univ, Dept Agr Sci, 3500 John Merritt Blvd, Nashville, TN 37209 USA.; Thannhauser, TW (reprint author), Cornell Univ, Funct & Comparat Prote Ctr, USDA ARS, Ithaca, NY 14853 USA. EM zye@my.tnstate.edu; sangisasi@gmail.com; iyk_oc@yahoo.com; zsuping@tnstate.edu; cyu@my.tnstate.edu; dhui@tnstate.edu; kjh46@cornell.edu; tlf26@cornell.edu; tt34@cornell.edu FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2012-02466, TENX-1507-SE]; U.S. Department of Agriculture Agricultural Research Service [1907-21000-036/037-00D] FX The authors wish to thank Sheng Zhang of the Proteomics and Mass Spectrometry Facility of the Cornell University Institute of Biotechnology for expert technical assistance and helpful discussion; Roger Sauve, Jason de Koff, Fur-Chi Chen, and George Smith at Tennessee State University for discussions in experimental design; and Mrs. Sarabjit Bhatti and Long Zhang for assisting in carrying out the study. This work was supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Grant No. 2012-02466, project TENX-1507-SE, and the U.S. Department of Agriculture Agricultural Research Service, Grant No. 1907-21000-036/037-00D. ARS disclaimer: "Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture." Seeds of switchgrass "Alamo" were kindly provided by Jason de Koff, Tennessee State University, Nashville, TN, USA. NR 92 TC 1 Z9 1 U1 14 U2 14 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1422-0067 J9 INT J MOL SCI JI Int. J. Mol. Sci. PD AUG PY 2016 VL 17 IS 8 AR 1251 DI 10.3390/ijms17081251 PG 18 WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary SC Biochemistry & Molecular Biology; Chemistry GA DU6PU UT WOS:000382337900064 ER PT J AU Eggleston, G Cole, M Toyamasu, T Triplett, A Montes, B Wartelle, L Stewart, D AF Eggleston, Gillian Cole, Marsha Toyamasu, Toshihisa Triplett, Alexa Montes, Belisario Wartelle, Lynda Stewart, David TI Conquering the control of insoluble and soluble starch with novel applications of amylase SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE Insoluble starch; soluble starch; sugarcane; amylase; viscosity; clarification; evaporation ID SEASONAL-VARIATIONS; SUGAR; RAW AB The new knowledge that there is markedly more insoluble starch than previously considered in products across both the sugarcane factory and refinery has processing implications. This includes the application of alpha-amylases in the factory to control not only soluble but insoluble starch. Studies were conducted at the laboratory, pilot plant, and factory scales to optimize the application of amylase for control of both starch forms. High-temperature (HT) stable amylases, and even intermediate-temperature (IT) stable amylases but to a lesser extent, are capable of hydrolyzing starch in 96 degrees C clarified juice mostly in the first 10 min before their substantial denaturation. Amylases preferentially hydrolyze soluble starch>>>>>swollen starch>insoluble starch. HT amylases compared to IT amylase, however, cause unwanted carry-over amylase activity even at a 1 ppm dose. At a Louisiana factory, novel combinations and doses (0 to 10 ppm) of an IT stable amylase were added to a clarification tank, next -to -the -last evaporator, and/or last evaporator. Using the new USDA starch research method to measure total, insoluble, and soluble starch, showed that the dynamics of starch transformation and removal during clarification and evaporation were much more complex than previously considered. When IT amylase is first directly applied into a clarification tank, total starch is reduced by 25.3 to 31.2% which includes insoluble starch, and the control of the syrup viscosity inside the subsequent evaporators is also improved. Although most starch hydrolysis occurred in the next-to-the-last evaporator syrups, the greatest overall reductions (up to 99% depending on enzyme dose) for all starch forms occur when IT amylase is simultaneously added into the clarification tank, next -to -the last evaporator, and last evaporator. Until a solution can be found to remove carry-over amylase, the latter treatment is recommended. C1 [Eggleston, Gillian; Cole, Marsha; Triplett, Alexa; Wartelle, Lynda] ARS, USDA, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. [Toyamasu, Toshihisa] Amano Enzyme Inc, Gifu R&D Ctr, 1-6 Technoplaza, Kakamigahara, Gifu 5090109, Japan. [Montes, Belisario; Stewart, David] Alma Plantat, 4612 Alma Rd, Lakeland, LA 70752 USA. RP Eggleston, G (reprint author), ARS, USDA, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov NR 19 TC 0 Z9 0 U1 7 U2 7 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD AUG PY 2016 VL 118 IS 1412 BP 572 EP 581 PG 10 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA DT2RB UT WOS:000381327000031 ER PT J AU Coopersmith, EJ Cosh, MH Jacobs, JM AF Coopersmith, Evan J. Cosh, Michael H. Jacobs, Jennifer M. TI Comparison of In Situ Soil Moisture Measurements: An Examination of the Neutron and Dielectric Measurements within the Illinois Climate Network SO JOURNAL OF ATMOSPHERIC AND OCEANIC TECHNOLOGY LA English DT Article ID PATTERNS AB The continuity of soil moisture time series data is crucial for climatic research. Yet, a common problem for continuous data series is the changing of sensors, not only as replacements are necessary, but as technologies evolve. The Illinois Climate Network has one of the longest data records of soil moisture; yet, it has a discontinuity when the primary sensor (neutron probes) was replaced with a dielectric sensor. Applying a simple model coupled with machine learning, the two time series can be merged into one continuous record by training the model on the latter dielectric model and minimizing errors against the former neutron probe dataset. The model is able to be calibrated to an accuracy of 0.050 m(3) m(-3) and applying this to the earlier series and applying a gain and offset, an RMSE of 0.055 m(3) m(-3) is possible. As a result of this work, there is now a singular network data record extending back to the 1980s for the state of Illinois. C1 [Coopersmith, Evan J.; Cosh, Michael H.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Coopersmith, Evan J.; Jacobs, Jennifer M.] Univ New Hampshire, Dept Civil & Environm Engn, Durham, NH 03824 USA. RP Coopersmith, EJ (reprint author), USDA, Hydrol & Remote Sensing Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM ecooper2@gmail.com FU NASA Terrestrial Hydrology Program [NNH10ZDA001N-THP]; USDA Agricultural Research Service [ARS 58-8042-5-077] FX This work was supported by the NASA Terrestrial Hydrology Program (NNH10ZDA001N-THP) and the USDA Agricultural Research Service (ARS 58-8042-5-077). USDA is an equal opportunity provider and employer. NR 27 TC 0 Z9 0 U1 3 U2 3 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0739-0572 EI 1520-0426 J9 J ATMOS OCEAN TECH JI J. Atmos. Ocean. Technol. PD AUG PY 2016 VL 33 IS 8 BP 1749 EP 1758 DI 10.1175/JTECH-D-16-0029.1 PG 10 WC Engineering, Ocean; Meteorology & Atmospheric Sciences SC Engineering; Meteorology & Atmospheric Sciences GA DV4LI UT WOS:000382896700012 ER PT J AU Chen, X Kumar, M Wang, R Winstral, A Marks, D AF Chen, Xing Kumar, Mukesh Wang, Rui Winstral, Adam Marks, Danny TI Assessment of the Timing of Daily Peak Streamflow during the Melt Season in a Snow-Dominated Watershed SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID HYDRAULIC CONDUCTIVITY; MOUNTAIN CATCHMENT; HYDROLOGIC IMPACTS; SUBSURFACE FLOW; ENERGY-BALANCE; UNITED-STATES; SOIL-WATER; MODEL; GROUNDWATER; MELTWATER AB Previous studies have shown that gauge-observed daily streamflow peak times (DPTs) during spring snowmelt can exhibit distinct temporal shifts through the season. These shifts have been attributed to three processes: 1) melt flux translation through the snowpack or percolation, 2) surface and subsurface flow of melt from the base of snowpacks to streams, and 3) translation of water flux in the streams to stream gauging stations. The goal of this study is to evaluate and quantify how these processes affect observed DPTs variations at the Reynolds Mountain East (RME) research catchment in southwest Idaho, United States. To accomplish this goal, DPTs were simulated for the RME catchment over a period of 25 water years using a modified snowmelt model, iSnobal, and a hydrology model, the Penn State Integrated Hydrologic Model (PIHM). The influence of each controlling process was then evaluated by simulating the DPT with and without the process under consideration. Both intra- and interseasonal variability in DPTs were evaluated. Results indicate that the magnitude of DPTs is dominantly influenced by subsurface flow, whereas the temporal shifts within a season are primarily controlled by percolation through snow. In addition to the three processes previously identified in the literature, processes governing the snowpack ripening time are identified as additionally influencing DPT variability. Results also indicate that the relative dominance of each control varies through the melt season and between wet and dry years. The results could be used for supporting DPTs prediction efforts and for prioritization of observables for DPT determination. C1 [Chen, Xing; Kumar, Mukesh; Wang, Rui] Duke Univ, Nicholas Sch Environm, 450 Res Dr,LSRC A207A, Durham, NC 27708 USA. [Winstral, Adam; Marks, Danny] ARS, Northwest Watershed Res Ctr, USDA, Boise, ID USA. [Winstral, Adam] WSL Inst Snow & Avalanche Res SLF, Davos, Switzerland. RP Kumar, M (reprint author), Duke Univ, Nicholas Sch Environm, 450 Res Dr,LSRC A207A, Durham, NC 27708 USA. EM mukesh.kumar@duke.edu FU NSF CAREER [EAR 1454983]; USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West [5362-13610-008-00D] FX Datasets used in the paper are publicly available from the Northwest Watershed Research Center, USDA anonymous ftp site (ftp://ftp.nwrc.ars.usda.gov/public/RME_25yr_database). The data and analysis presented in this paper were funded in part by NSF CAREER award (EAR 1454983) and USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West (5362-13610-008-00D). We thank Jeff Dozier and Jessica Lundquist for providing constructive comments that greatly improved this manuscript. NR 77 TC 0 Z9 0 U1 5 U2 5 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD AUG PY 2016 VL 17 IS 8 BP 2225 EP 2244 DI 10.1175/JHM-D-15-0152.1 PG 20 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DW0DG UT WOS:000383310500007 ER PT J AU Yilmaz, MT Crow, WT Ryu, D AF Yilmaz, M. T. Crow, W. T. Ryu, D. TI Impact of Model Relative Accuracy in Framework of Resealing Observations in Hydrological Data Assimilation Studies SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID SATELLITE SOIL-MOISTURE; LAND DATA ASSIMILATION; SURFACE MODEL; VALIDATION; PRODUCTS; STABILITY; SYSTEM; ERROR AB Soil moisture datasets vary greatly with respect to their time series variability and signal-to-noise characteristics. Minimizing differences in signal variances is particularly important in data assimilation to optimize the accuracy of the analysis obtained after merging model and observation datasets. Strategies that reduce these differences are typically based on resealing the observation time series to match the model. As a result, the impact of the relative accuracy of the model reference dataset is often neglected. In this study, the impacts of the relative accuracies of model- and observation-Pbased soil moisture time series for seasonal and sub seasonal (anomaly) components, respectively on optimal model-Pobservation integration are investigated. Experiments are performed using both well-Pcontrolled synthetic and real data test beds. Investigated experiments are based on resealing observations to a model using strategies with decreasing aggressiveness: 1) using the seasonality of the model directly while matching the variance of the observed anomaly component, 2) resealing the seasonality and the anomaly components separately, and 3) resealing the entire time series as one piece or for each monthly climatology. All experiments use a simple antecedent precipitation index model and assimilate observations via a Kalman filtering approach. Synthetic and real data assimilation results demonstrate that resealing observations more aggressively to the model is favorable when the model is more skillful than observations; however, resealing observations more aggressively to the model can degrade the Kalman filter analysis if observations are relatively more accurate. C1 [Yilmaz, M. T.] Middle East Tech Univ, Dept Civil Engn, Eskisehir 7 Km,ODTU Insaat Muh,K4-109, TR-06800 Ankara, Turkey. [Crow, W. T.] USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Ryu, D.] Univ Melbourne, Dept Infrastruct Engn, Parkville, Vic, Australia. RP Yilmaz, MT (reprint author), Middle East Tech Univ, Dept Civil Engn, Eskisehir 7 Km,ODTU Insaat Muh,K4-109, TR-06800 Ankara, Turkey. EM tuyilmaz@metu.edu.tr FU EU [630110]; Scientific and Technological Research Council of Turkey (TUBITAK) [3501, 114Y676] FX We thank Michael Cosh (michael.cosh@ars.usda.gov) of the U.S. Department of Agriculture for USDA ARS watershed soil moisture data sets, Robert Parinussa (r.m.parinussa@vu.nl) of Vrije Universiteit Amsterdam for LPRM datasets, and NASA Goddard Earth Sciences (GES) Data and Information Services Center (DISC) for Noah and TRMM datasets (http://disc.sci.gsfc.nasa.gov/). These data can be acquired from their respective sources. We also thank the reviewers for their constructive comments which improved the presentation of the paper. Research was supported by EU Marie Curie Seventh Framework Programme FP7-PEOPLE-2013-CIG project number 630110 (principal investigator, M. Tugrul Yilmaz) and the Scientific and Technological Research Council of Turkey (TUBITAK) Grant 3501, Project 114Y676 (principal investigator, M. Tugrul Yilmaz). NR 32 TC 0 Z9 0 U1 0 U2 0 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD AUG PY 2016 VL 17 IS 8 BP 2245 EP 2257 DI 10.1175/JHM-D-15-0206.1 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DW0DG UT WOS:000383310500008 ER PT J AU Shellito, PJ Small, EE Cosh, MH AF Shellito, Peter J. Small, Eric E. Cosh, Michael H. TI Calibration of Noah Soil Hydraulic Property Parameters Using Surface Soil Moisture from SMOS and Basinwide In Situ Observations SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID RAINFALL-RUNOFF MODELS; REMOTELY-SENSED DATA; LAND-SURFACE; MULTIOBJECTIVE CALIBRATION; PEDOTRANSFER FUNCTIONS; HYDROLOGIC-MODELS; WATER-FLOW; GLOBAL OPTIMIZATION; SPATIAL-RESOLUTION; SENSITIVITY AB Soil hydraulic properties (SHPs) control infiltration and redistribution of moisture in a soil column. The Noah land surface model (LSM) default simulation selects SHPs according to a location's mapped soil texture class. SHPs are instead estimated at seven sites in North America through calibration. A single-objective algorithm minimizes the root-mean-square difference (RMSD) between simulated surface soil moisture and observations from 1) a dense network of in situ probes, 2) Soil Moisture Ocean Salinity (SMOS) satellite retrievals, and 3) SMOS retrievals adjusted such that their mean equals that of the in situ network. Parameters are optimized in 2012 and validated in 2013 against the in situ network. RMSD and unbiased RMSD (ubRMSD) assess resulting surface soil moisture behavior. At all sites, assigning SHP parameters from a different soil texture than the one that is mapped decreases the RMSD by an average of 0.029 cm(3) cm(-3). Similar improvements result from calibrating parameters using in situ network data (0.031 cm(3) cm(-3)). Calibrations using remotely sensed data show comparable success (0.029 cm(3) cm(-3)) if the SMOS product has no bias. Calibrated simulations are superior to texture-based simulations in their ability to decrease ubRMSD at times of year when the default simulation is worst. Changes to both RMSD and ubRMSD are small when the default simulation is already good. Most calibrated simulations have higher runoff ratios than do texture based simulations, a change that warrants further evaluation. Overall, parameter selection using SMOS data shows good potential where biases are low. C1 [Shellito, Peter J.; Small, Eric E.] Univ Colorado Boulder, Dept Geol Sci, UCB 399, Boulder, CO 80309 USA. [Cosh, Michael H.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD USA. RP Shellito, PJ (reprint author), Univ Colorado Boulder, Dept Geol Sci, UCB 399, Boulder, CO 80309 USA. EM peter.shellito@colorado.edu FU NASA [NNX13AF43G] FX This research has been supported by NASA Grant NNX13AF43G. We thank two anonymous reviewers for their helpful comments. NR 92 TC 0 Z9 0 U1 5 U2 5 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X EI 1525-7541 J9 J HYDROMETEOROL JI J. Hydrometeorol. PD AUG PY 2016 VL 17 IS 8 BP 2275 EP 2292 DI 10.1175/JHM-D-15-0153.1 PG 18 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA DW0DG UT WOS:000383310500010 ER PT J AU Webster, CC Noakes, TD Chacko, SK Swart, J Kohn, TA Smith, JAH AF Webster, Christopher C. Noakes, Timothy D. Chacko, Shaji K. Swart, Jeroen Kohn, Tertius A. Smith, James A. H. TI Gluconeogenesis during endurance exercise in cyclists habituated to a long-term low carbohydrate high-fat diet SO JOURNAL OF PHYSIOLOGY-LONDON LA English DT Article ID ISOTOPOMER DISTRIBUTION ANALYSIS; ENDOGENOUS GLUCOSE-PRODUCTION; MUSCLE GLYCOGEN; SKELETAL-MUSCLE; HEALTHY-MEN; PHYSICAL PERFORMANCE; SUBSTRATE OXIDATION; MEASUREMENT ERROR; TRAINED CYCLISTS; LIPID-METABOLISM AB Endogenous glucose production (EGP) occurs via hepatic glycogenolysis (GLY) and gluconeogenesis (GNG) and plays an important role in maintaining euglycaemia. Rates of GLY and GNG increase during exercise in athletes following a mixed macronutrient diet; however, these processes have not been investigated in athletes following a low carbohydrate high fat (LCHF) diet. Therefore, we studied seven well-trained male cyclists that were habituated to either a LCHF (7% carbohydrate, 72% fat, 21% protein) or a mixed diet (51% carbohydrate, 33% fat, 16% protein) for longer than 8 months. After an overnight fast, participants performed a 2 h laboratory ride at 72% of maximal oxygen consumption. Glucose kinetics were measured at rest and during the final 30 min of exercise by infusion of [6,6-H-2(2)]-glucose and the ingestion of (H2O)-H-2 tracers. Rates of EGP and GLY both at rest and during exercise were significantly lower in the LCHF group than the mixed diet group (Exercise EGP: LCHF, 6.0 +/- 0.9 mg kg(-1) min(-1), Mixed, 7.8 +/- 1.1 mg kg(-1) min(-1), P < 0.01; Exercise GLY: LCHF, 3.2 +/- 0.7 mg kg(-1) min(-1), Mixed, 5.3 +/- 0.9 mg kg(-1) min(-1), P < 0.01). Conversely, no difference was detected in rates of GNG between groups at rest or during exercise (Exercise: LCHF, 2.8 +/- 0.4 mg kg(-1) min(-1), Mixed, 2.5 +/- 0.3 mg kg(-1) min(-1), P = 0.15). We conclude that athletes on a LCHF diet do not compensate for reduced glucose availability via higher rates of glucose synthesis compared to athletes on a mixed diet. Instead, GNG remains relatively stable, whereas glucose oxidation and GLY are influenced by dietary factors. C1 [Webster, Christopher C.; Noakes, Timothy D.; Swart, Jeroen; Kohn, Tertius A.; Smith, James A. H.] Univ Cape Town, Div Exercise Sci & Sports Med ESSM, Dept Human Biol, Fac Hlth Sci, Newlands, South Africa. [Chacko, Shaji K.] ARS, Dept Pediat, Childrens Nutr Res Ctr, USDA,Baylor Coll Med, Houston, TX USA. RP Smith, JAH (reprint author), Private Bag X3, ZA-7701 Cape Town, South Africa. EM ja.smith@uct.ac.za RI Noakes, Timothy/E-7253-2011 OI Noakes, Timothy/0000-0001-7244-2375 FU National Research Foundation of South Africa; National Research Foundation [82434, 91443]; National Research Foundation S&F - Scarce Skills Masters Scholarships [90004] FX This research was funded by the National Research Foundation of South Africa; National Research Foundation Lifetime Achiever Grant 82434, Tim Noakes 2012; National Research Foundation Research Career Award 91443 James Smith, 2013; and National Research Foundation S&F - Scarce Skills Masters Scholarships 90004 Christopher Webster. NR 71 TC 2 Z9 2 U1 23 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-3751 EI 1469-7793 J9 J PHYSIOL-LONDON JI J. Physiol.-London PD AUG 1 PY 2016 VL 594 IS 15 BP 4389 EP 4405 DI 10.1113/JP271934 PG 17 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA DV5GQ UT WOS:000382955500032 PM 26918583 ER PT J AU AbuHammad, WA Mamidi, S Kumar, A Pirseyedi, S Manthey, FA Kianian, SF Alamri, MS Mergoum, M Elias, EM AF AbuHammad, Wesam A. Mamidi, Sujan Kumar, Ajay Pirseyedi, Seyed Manthey, Frank A. Kianian, Shahryar F. Alamri, Mohammed S. Mergoum, Mohamed Elias, Elias M. TI Identification and validation of a major cadmium accumulation locus and closely associated SNP markers in North Dakota durum wheat cultivars SO MOLECULAR BREEDING LA English DT Article DE Association mapping; Cadmium (Cd); Durum wheat; KASPar assay; Quantitative trait locus (QTL); Single nucleotide polymorphism ID QUANTITATIVE TRAIT LOCUS; NEAR-ISOGENIC LINES; GENOME-WIDE ASSOCIATION; ORYZA-SATIVA; WINTER-WHEAT; GRAIN; RICE; TRANSLOCATION; CD; PLANT AB Durum wheat has the tendency of accumulating more cadmium (Cd), a biotoxic heavy metal, in seeds than other commonly grown cereals, thus posing a serious food safety/public health concern. This could have serious negative impact on the national pasta industry and the international export market of durum wheat. The phenotyping for selecting low Cd lines is expensive and time consuming. The use of markers could be a more sustainable approach for selecting lines with low Cd levels. Here, a RIL population developed from a cross between Grenora (high Cd) 9 Haurani (low Cd) and two association mapping panels consisting of advanced breeding lines from the North Dakota durum wheat breeding program were used to identify QTL and associated markers for Cd. A major QTL, with Haurani contributing low Cd uptake allele and explaining 54.3 % phenotypic variation, was detected on chromosome 5BL. Association mapping using 2010 collection validated the results of linkage mapping and identified major QTL on 5BL. The 2009 collection, showed the presence of a major QTL on chromosome 2B. The SNP marker associated with major QTL on 5BL was converted to user friendly KASPar assay. The major QTL and associated KASPar marker were further validated using another RIL population developed from a cross of Strongfield (low Cd) and Alkabo (high Cd). The development of suitable marker assay, associated with major Cd uptake QTL, would help the selection for low Cd accumulating lines, minimizing the costly phenotypic evaluation for this important trait. C1 [AbuHammad, Wesam A.; Mamidi, Sujan; Kumar, Ajay; Pirseyedi, Seyed; Manthey, Frank A.; Mergoum, Mohamed; Elias, Elias M.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [AbuHammad, Wesam A.] Arizona Plant Breeders Inc, Casa Grande, AZ 85122 USA. [Mamidi, Sujan] HudsonAlpha Inst Biotechnol, Huntsville, AL USA. [Kianian, Shahryar F.] ARS, Cereal Dis Lab, USDA, St Paul, MN 55108 USA. [Alamri, Mohammed S.] King Saud Univ, Nutr Coll Food & Agr Sci, Riyadh, Saudi Arabia. RP Elias, EM (reprint author), North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. EM elias.elias@ndsu.edu OI mamidi, sujan/0000-0002-3837-6121 FU North Dakota Wheat Commission FX The authors would like to thank Stan Stancyk and Sally Mann of the NDSU Plant Sciences Department for their assistance in planting and harvesting. Many thanks go to Dr. Shiaoman Chao, Dawn Feltus, Richard Sonju and Mary Osenga of the USDA for using their lab for genotyping. We thank the North Dakota Wheat Commission for funding this project. NR 98 TC 0 Z9 0 U1 20 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2016 VL 36 IS 8 AR 112 DI 10.1007/s11032-016-0536-1 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DU3XD UT WOS:000382144700006 ER PT J AU Fresnedo-Ramirez, J Sun, Q Hwang, CF Ledbetter, CA Ramming, DW Fennell, AY Walker, MA Luby, JJ Clark, MD Londo, JP Cadle-Davidson, L Zhong, GY Reisch, BI AF Fresnedo-Ramirez, Jonathan Sun, Qi Hwang, Chin-Feng Ledbetter, Craig A. Ramming, David W. Fennell, Anne Y. Walker, M. Andrew Luby, James J. Clark, Matthew D. Londo, Jason P. Cadle-Davidson, Lance Zhong, Gan-Yuan Reisch, Bruce I. TI Toward the elucidation of cytoplasmic diversity in North American grape breeding programs SO MOLECULAR BREEDING LA English DT Article DE Plastid genome; Mitochondria genome; Cytoplasmic differentiation; Cytoplasmic lineage; Organelle genes; Vitis; Grapevine ID VITIS-VINIFERA; MITOCHONDRIAL-DNA; NUCLEAR GENOME; GENE-TRANSFER; POLYMORPHISMS; ANTHOCYANINS; POPULATIONS; INHERITANCE; SEQUENCES; EVOLUTION AB Plants have an intriguing tripartite genetic system: Nuclear genome x Mitochondria x Plastids and their interactions may impact germplasm breeding. In grapevine, the study of cytoplasmic genomes has been limited, and their role with respect to grapevine germplasm diversity has yet to be elucidated. In the present study, the results of an analysis of the cytoplasmic diversity among 6073 individuals (comprising cultivars, interspecific hybrids and segregating progenies) are presented. Genotyping by sequencing (GBS) was used to elucidate plastid and mitochondrial DNA sequences, and results were analyzed using multivariate techniques. Single nucleotide polymorphism (SNP) effects were annotated in reference to plastid and mitochondrial genome sequences. The cytoplasmic diversity identified was structured according to synthetic domestication groups (wine and raisin/table gr.ape types) and interspecific-hybridization-driven groups with introgression from North American Vitis species, identifying five cytoplasmic groups and four major clusters. Fifty-two SNP markers were used to describe the diversity of the germplasm. Ten organelle genes showed distinct SNP annotations and effect predictions, of which six were chloroplast-derived and three were mitochondrial genes, in addition to one mitochondrial SNP affecting a nonannotated open reading frame. The results suggest that the application of GBS will aid in the study of cytoplasmic genomes in grapevine, which will enable further studies on the role of cytoplasmic genomes in grapevine germplasm, and then allow the exploitation of these sources of diversity in breeding. C1 [Fresnedo-Ramirez, Jonathan; Sun, Qi] Cornell Univ, Inst Biotechnol, BRC Bioinformat Facil, Ithaca, NY 14853 USA. [Hwang, Chin-Feng] Missouri State Univ, Darr Sch Agr, State Fruit Expt Stn, Mt Grove Campus, Springfield, MO 65897 USA. [Ledbetter, Craig A.; Ramming, David W.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Fennell, Anne Y.] South Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Walker, M. Andrew] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Luby, James J.; Clark, Matthew D.] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Londo, Jason P.; Cadle-Davidson, Lance; Zhong, Gan-Yuan] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14456 USA. [Reisch, Bruce I.] Cornell Univ, Hort Sect, Sch Integrat Plant Sci, Geneva, NY 14456 USA. [Ramming, David W.] Rammings Specialty Crops, Fresno, CA 93737 USA. [Fresnedo-Ramirez, Jonathan] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Fresnedo-Ramirez, J (reprint author), Cornell Univ, Inst Biotechnol, BRC Bioinformat Facil, Ithaca, NY 14853 USA.; Fresnedo-Ramirez, J (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. EM fresnedoramirez.1@osu.edu; qisun@cornell.edu; ChinFengHwang@missouristate.edu; Craig.Ledbetter@ars.usda.gov; dwramming@yahoo.com; Anne.Fennell@sdstate.edu; awalker@ucdavis.edu; lubyx001@umn.edu; clark776@umn.edu; jason.londo@ars.usda.gov; lance.cadledavidson@ars.usda.gov; GanYuan.Zhong@ars.usda.gov; bruce.reisch@cornell.edu OI Fresnedo Ramirez, Jonathan/0000-0003-1985-2513; Clark, Matthew/0000-0002-1771-1955 FU U.S. Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative [2011-51181-30635]; National Grape and Wine Initiative FX The authors acknowledge the U.S. Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Research Initiative (Award no. 2011-51181-30635), and the National Grape and Wine Initiative for funding for the VitisGen project (http://www.vitisgen.org/). We also thank Shanshan Yang for discussion and suggestions. NR 42 TC 0 Z9 0 U1 15 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2016 VL 36 IS 8 AR 116 DI 10.1007/s11032-016-0538-z PG 10 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DU3XD UT WOS:000382144700004 ER PT J AU Del Coco, VF Sparo, MD Sidoti, A Santin, M Basualdo, JA Cordoba, MA AF Del Coco, Valeria F. Sparo, Monica D. Sidoti, Alicia Santin, Monica Angel Basualdo, Juan Alejandra Cordoba, Maria TI Effects of Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in mice SO PARASITOLOGY RESEARCH LA English DT Article DE Cryptosporidium; Probiotics; Immunosuppressed mice; Enterococcus faecalis CECT7121 ID GIARDIA-INTESTINALIS; LACTOBACILLUS-REUTERI; OOCYSTS; CHILDREN AB Cryptosporidium is an opportunistic protozoan parasite of humans and animals worldwide and causes diarrheal disease that is typically self-limiting in immunocompetent hosts but often life threatening to immunocompromised individuals. However, there is a lack of completely efficient therapy available. Probiotics have attracted the attention as potential antiparasite compounds against protozoa involved in intestinal infections. This study investigated the effects of administration of probiotic Enterococcus faecalis CECT 7121 on Cryptosporidium parvum infection in immunosuppressed mice. Effects on C. parvum infection at the intestinal mucosa were studied and scored at each portion of the gut. It was demonstrated that Ef CECT 7121 interfered with C. parvum infection when both probiotic and parasite were present in the same intestinal location suggesting that Ef CECT 7121 supplementation can alleviate the negative effects of C. parvum infection. C1 [Del Coco, Valeria F.; Sparo, Monica D.; Angel Basualdo, Juan; Alejandra Cordoba, Maria] Univ Nacl La Plata, Fac Ciencias Med, Ctr Estudios Microbiol & Parasitol CUDEMyP, 60 & 120, RA-1900 La Plata, Buenos Aires, Argentina. [Del Coco, Valeria F.] Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina. [Sidoti, Alicia] Univ Nacl La Plata, Fac Ciencias Med, Catedra Patol B, Buenos Aires, DF, Argentina. [Santin, Monica] USDA, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Alejandra Cordoba, Maria] Comis Invest Cient Prov Buenos Aires, Buenos Aires, DF, Argentina. RP Del Coco, VF (reprint author), Univ Nacl La Plata, Fac Ciencias Med, Ctr Estudios Microbiol & Parasitol CUDEMyP, 60 & 120, RA-1900 La Plata, Buenos Aires, Argentina.; Del Coco, VF (reprint author), Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina. EM valeriadelcoco@med.unlp.edu.ar FU Universidad Nacional de La Plata FX This work was supported by Universidad Nacional de La Plata. NR 31 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD AUG PY 2016 VL 115 IS 8 BP 3239 EP 3244 DI 10.1007/s00436-016-5087-1 PG 6 WC Parasitology SC Parasitology GA DS9EP UT WOS:000381085900037 PM 27193238 ER PT J AU Martin, LBB Sherwood, RW Nicklay, JJ Yang, Y Muratore-Schroeder, TL Anderson, ET Thannhauser, TW Rose, JKC Zhang, S AF Martin, Laetitia B. B. Sherwood, Robert W. Nicklay, Joshua J. Yang, Yong Muratore-Schroeder, Tara L. Anderson, Elizabeth T. Thannhauser, Theodore W. Rose, Jocelyn K. C. Zhang, Sheng TI Application of wide selected-ion monitoring data-independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor SO PROTEOMICS LA English DT Article DE Data-independent acquisition; Label-free quantitative proteomics; Plant cuticle; Plant proteomics; Solanum lycopersicum; Wide selected-ion monitoring ID PROTEOMIC ANALYSIS; MASS-SPECTROMETRY; PLANT; CELL; QUANTITATION; QUANTIFICATION; ARABIDOPSIS; EXPRESSION; ABUNDANCE; SKYLINE AB We describe here the use of label-free wide selected-ion monitoring data-independent acquisition (WiSIM-DIA) to identify proteins that are involved in the formation of tomato (Solanum lycopersicum) fruit cuticles and that are regulated by the transcription factor CUTIN DEFICIENT2 (CD2). A spectral library consisting of 11753 unique peptides, corresponding to 2338 tomato protein groups, was used and the DIA analysis was performed at the MS1 level utilizing narrow mass windows for extraction with Skyline 2.6 software. We identified a total of 1140 proteins, 67 of which had expression levels that differed significantly between the cd2 tomato mutant and the wild-type cultivar M82. Differentially expressed proteins including a key protein involved in cutin biosynthesis, were selected for validation by target SRM/MRM and by Western blot analysis. In addition to confirming a role for CD2 in regulating cuticle formation, the results also revealed that CD2 influences pathways associated with cell wall biology, anthocyanin biosynthesis, plant development, and responses to stress, which complements findings of earlier RNA-Seq experiments. Our results provide new insights into molecular processes and aspects of fruit biology associated with CD2 function, and demonstrate that the WiSIM-DIA is an effective quantitative approach for global protein identifications. C1 [Martin, Laetitia B. B.; Rose, Jocelyn K. C.] Cornell Univ, Plant Biol Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA. [Sherwood, Robert W.; Anderson, Elizabeth T.; Zhang, Sheng] Cornell Univ, Prote & Mass Spectrometry Facil, Inst Biotechnol, Ithaca, NY 14850 USA. [Nicklay, Joshua J.; Muratore-Schroeder, Tara L.] Thermo Fisher Sci, Somerset, NJ USA. [Yang, Yong; Thannhauser, Theodore W.] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Rose, JKC (reprint author), Cornell Univ, Plant Biol Sect, Sch Integrat Plant Sci, Ithaca, NY 14853 USA.; Zhang, S (reprint author), Cornell Univ, Prote & Mass Spectrometry Facil, Inst Biotechnol, Ithaca, NY 14850 USA. EM jr286@cornell.edu; sz14@cornell.edu FU NIH SIG Grant [1S10 OD017992-01]; US Department of Agriculture Cooperative State Research, Education, and Extension Service [2011-04197, 2015-06803]; US National Science Foundation (Plant Genome Research Program) [IOS-1339287] FX We want to thank Nicholas Segerson for his help with protein extraction. This work was partially supported by NIH SIG Grant 1S10 OD017992-01 to S.Z., and by US Department of Agriculture Cooperative State Research, Education, and Extension Service (2011-04197 and 2015-06803) and US National Science Foundation (Plant Genome Research Program; IOS-1339287) grants to J.K.C.R. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 37 TC 1 Z9 1 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1615-9853 EI 1615-9861 J9 PROTEOMICS JI Proteomics PD AUG PY 2016 VL 16 IS 15-16 SI SI BP 2081 EP 2094 DI 10.1002/pmic.201500450 PG 14 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA DV9RV UT WOS:000383280000003 PM 27089858 ER PT J AU Ulrey, C Quintana-Ascencio, PF Kauffman, G Smith, AB Menges, ES AF Ulrey, Christopher Quintana-Ascencio, Pedro F. Kauffman, Gary Smith, Adam B. Menges, Eric S. TI Life at the top: Long-term demography, microclimatic refugia, and responses to climate change for a high-elevation southern Appalachian endemic plant SO BIOLOGICAL CONSERVATION LA English DT Article DE Blue Ridge Mountains; Climate modeling; Geum radiatum; Rare species demography; Integral projection modeling; Niche modeling ID INTEGRAL PROJECTION MODELS; MONTANE CLOUD FORESTS; SPRUCE-FIR FOREST; SPECIES DISTRIBUTIONS; UNITED-STATES; NORTH-AMERICA; RANGE SHIFTS; IMMERSION; CONSERVATION; BIODIVERSITY AB Plants existing in small and isolated populations often depend on microclimatic refugia that create local environments buffered from macroclimatic conditions. Currently much effort is devoted toward identifying features that create refugial conditions in the expectation that they will continue to serve as refugia into the future. However, the ability of a refuge to resist macroclimatic change is a biological question, not an abiotic one, since species can persist in these conditions while suffering slow decline. Here we test the ability of current refugial habitats of Geum radiatum, a narrowly endemic perennial herb specializing in cool, humid, high elevation sites, to continue to serve as refugia under present and future climatic conditions. We constructed integral projection models to characterize demography, and macro-and topoclimatic niche models to predict dynamics given climate change through 2070. This species' demography is characterized by high adult survival (about 97% annually), variable growth, frequent flowering, and rare seedling recruitment. Site relative humidity affected survival and reproduction, but predicted population growth rates under current conditions were similar for dry, wet, sheltered, and exposed sites (lambda = 0.994-0.998). Augmentation by planting 20-70 seedlings annually would raise population growth rates to 1. Demographic modeling under future lower relative humidity predicted further reductions in population growth. Models of the species' macro-and microclimatic niche indicated that all populations had reduced climatic suitability by 2050 or 2080, with 58-83% falling below minimum suitability levels, depending on the climate scenario. G. radiatum's stable demography and habitat protection mean that, barring catastrophes, most populations are not facing extinction under current conditions. However, this species is extremely vulnerable to projected climate change even within its current refugial habitats. This study demonstrates that climate refugia that currently buffer rare species from macroclimatic extremes may not be able to do so under anticipated climate change. (C) 2016 Published by Elsevier Ltd. C1 [Ulrey, Christopher] Natl Pk Serv, Asheville, NC 28803 USA. [Quintana-Ascencio, Pedro F.] Univ Cent Florida, Dept Biol, Orlando, FL 32816 USA. [Kauffman, Gary] US Forest Serv, Asheville, NC 28801 USA. [Smith, Adam B.] Missouri Bot Garden, Ctr Conservat & Sustainable Dev, 4344 Shaw Blvd, St Louis, MO 63110 USA. [Menges, Eric S.] Archbold Biol Stn, Plant Ecol Program, Venus, FL 33960 USA. RP Menges, ES (reprint author), Archbold Biol Stn, Plant Ecol Program, Venus, FL 33960 USA. EM emenges@archbold-station.org OI Smith, Adam/0000-0002-6420-1659 FU Blue Ridge Parkway Foundation; National Fish and Wildlife Foundation [2005-0011-003] FX The authors thank the US Fish and Wildlife Service, the US Forest Service, and Archbold Biological Station for providing personnel support. We also appreciate small grants from the Blue Ridge Parkway Foundation (2005 grant) and the National Fish and Wildlife Foundation (grant 2005-0011-003). We appreciate access to field sites granted by the US Forest Service, the Blue Ridge Parkway, the city of Waynesville, the Great Smoky Mountains National Park, Grandfather Mountain, and private landowners. Matthew Albrecht provided useful ideas on climate modeling. Field assistance from Marshall Ellis, Matt Levine, Sarah Fraser, Kent Menges, Viani Menges, Carolyn Wells Swed, M. Alexander, Ilex McLeod, Margaret McLeod, Linda Rodriguez-Torrent, J. Pope, Janet Rock and many others is greatly appreciated. Thanks to Ron Lance for propagation expertise. NR 80 TC 1 Z9 1 U1 21 U2 21 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD AUG PY 2016 VL 200 BP 80 EP 92 DI 10.1016/j.biocon.2016.05.028 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DT2QK UT WOS:000381325300010 ER PT J AU Zheng, Y Zhang, RH Pan, ZL AF Zheng, Yi Zhang, Ruihong Pan, Zhongli TI Investigation of adsorption kinetics and isotherm of cellulase and beta-glucosidase on lignocellulosic substrates SO BIOMASS & BIOENERGY LA English DT Article DE Adsorption kinetics; Adsorption isotherm; Cellulase; beta-glucosidase; Creeping wild ryegrass; Tween 20 ID TRICHODERMA-REESEI CELLULASE; CREEPING WILD RYEGRASS; ENZYMATIC-HYDROLYSIS; PRETREATED LIGNOCELLULOSE; POLY(ETHYLENE GLYCOL); CELLOBIOHYDROLASE I; ENDOGLUCANASE II; LIGNIN; ENZYMES; SOFTWOOD AB Clear understanding of enzyme adsorption during enzymatic hydrolysis of lignocellulosic biomass is essential to enhance the cost-efficiency of hydrolysis. However, conclusions from literature often contradicted each other because enzyme adsorption is enzyme, biomass/pretreatment and experimental condition specific, which makes descriptions and modeling of enzyme-substrate interaction difficult and inconsistent from case to case. This study investigated adsorption kinetics and isotherm under actual hydrolysis conditions with commercial cellulase and beta-glucosidase on Avicel, dilute acid pretreated Creeping Wild Ryegrass (pCWR) and lignin residue of pCWR after enzymatic hydrolysis. It was found that b-glucosidase has little affinity to Avicel, but significant affinity to dilute acid pCWR and lignin with maximum adsorption capacity (E-max) of 161.57 and 173.50 mg protein/g-substrate, respectively. During hydrolysis, adsorption of cellulase on Avicel was productive and reversible (E-max = 22.86 mg protein/g-substrate); however, nonproductive and irreversible adsorption of cellulase on pCWR (E-max = 42.55 mg protein/g-substrate) and lignin (E-max = 86.07 mg protein/g-substrate) became significant and resulted in cellulase deactivation. Lignin is a key issue causing high cost of enzymatic hydrolysis of lignocellulosic biomass. The nonionic surfactant, Tween 20 was found to significantly overcome nonproductive adsorption of cellulase and b-glucosidase on lignin by reducing the adsorption affinity. All adsorption data including with and without Tween 20 were fit well to Langmuir isotherm. The results from this research will provide useful data for model development of enzymatic hydrolysis. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Zheng, Yi] Clemson Univ, Dept Environm Engn & Earth Sci, 342 Comp Court, Anderson, SC 29625 USA. [Zhang, Ruihong; Pan, Zhongli] Univ Calif Davis, Dept Biol & Agr Engn, One Shields Ave, Davis, CA 95616 USA. [Pan, Zhongli] USDA ARS WRRC, Proc Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. RP Zheng, Y (reprint author), Clemson Univ, Dept Environm Engn & Earth Sci, 342 Comp Court, Anderson, SC 29625 USA. EM zheng9@clemson.edu FU California Department of Water Resources [4600002991] FX The authors would like to thank Novozymes, Inc. (Davis, CA) for providing enzymes for this research. The funding support for this research was provided by a research grant from California Department of Water Resources (Grant No. 4600002991). NR 38 TC 0 Z9 0 U1 16 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD AUG PY 2016 VL 91 BP 1 EP 9 DI 10.1016/j.biombioe.2016.04.014 PG 9 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DU9GI UT WOS:000382524100001 ER PT J AU Igathinathane, C Tumuluru, JS Keshwani, D Schmer, M Archer, D Liebig, M Halvorson, J Hendrickson, J Kronberg, S AF Igathinathane, C. Tumuluru, J. S. Keshwani, D. Schmer, M. Archer, D. Liebig, M. Halvorson, J. Hendrickson, J. Kronberg, S. TI Biomass bale stack and field outlet locations assessment for efficient infield logistics SO BIOMASS & BIOENERGY LA English DT Article DE Bioenergy feedstocks; Biomass utilization; Farm machinery; Field operation; Infield storage; Renewable energy ID SUPPLY ANALYSIS; CORN STOVER; MODEL; IBSAL AB Harvested hay or biomass are traditionally baled for better handling and they are transported to the outlet for final utilization. For better management of bale logistics, producers often aggregate bales into stacks so that bale-hauling equipment can haul multiple bales for improved efficiency. Objectives of this research include simulation of bale collection logistics after forming subfield stacks, evaluation of location effects of bale stack and field outlet, the number of stacks, transported bales/trip, and other field parameters on logistics distances (aggregation, transportation, and total). The software `R' performed the simulation, statistical analysis, and data visualization. Formation of bale stacks decoupled aggregation and transportation components. Stacks formation thus allows for aggregation and transportation to be performed at different times. Increasing the number of subfield stacks and the number of transported bales/trip significantly reduced the total logistics distances. The order for the best bale stack and outlet locations was: middle, near middle, mid-edge along the length, mid-edge along the width, and finally, corners. Except for swath and windrow variation, the studied field variables had a highly significant influence on the logistics distances. Increased bales/trip (>= 6) reduced the variations of outlet locations. Locating the field outlet at or near the center of the field along with an appropriate number of square subfields with stacks at the middle, and increased bales/trip will be the most efficient infield logistics strategy. Published by Elsevier Ltd. C1 [Igathinathane, C.] North Dakota State Univ, Dept Agr & Biosyst Engn, 1221 Albrecht Blvd, Fargo, ND 58102 USA. [Tumuluru, J. S.] Idaho Natl Lab, Biofuels & Renewable Energy Technol Dept, 750 Univ Blvd, Idaho Falls, ID 83415 USA. [Keshwani, D.] Univ Nebraska, Dept Biol Syst Engn, 215 LW Chase Hall,East Campus, Lincoln, NE 68583 USA. [Schmer, M.] Univ Nebraska, USDA ARS AMRU, 251 Filley Hall Food Ind Complex,East Campus, Lincoln, NE 68583 USA. [Archer, D.; Liebig, M.; Halvorson, J.; Hendrickson, J.; Kronberg, S.] USDA ARS, Northern Great Plains Res Lab, 1701 10th Ave SW, Mandan, ND 58554 USA. RP Igathinathane, C (reprint author), North Dakota State Univ, Dept Agr & Biosyst Engn, 1221 Albrecht Blvd, Fargo, ND 58102 USA. EM Igathinathane.Cannayen@ndsu.edu FU USDA National Institute of Food and Agriculture [ND01472, 229896] FX This work supported in part by the USDA National Institute of Food and Agriculture, Hatch Project: ND01472, Accession number: 229896. Typing of parts of the manuscript and discussion support extended by I. Srividhya is also appreciated. NR 18 TC 3 Z9 3 U1 3 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD AUG PY 2016 VL 91 BP 217 EP 226 DI 10.1016/j.biombioe.2016.05.019 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA DU9GI UT WOS:000382524100024 ER PT J AU Gaither, CJ Himmelfarb, D Hitchner, S Schelhas, J Shepherd, JM Binita, KC AF Gaither, Cassandra Johnson Himmelfarb, David Hitchner, Sarah Schelhas, John Shepherd, J. Marshall Binita, K. C. TI "Where the Sidewalk Ends": Sustainable Mobility in Atlanta's Cascade Community SO CITY & SOCIETY LA English DT Article DE Climate Change; African American Communities; Atlanta; GA ID CLIMATE-CHANGE; GEORGIA; POLITICS AB Roughly one third of U.S. greenhouse gas emissions are travel-related, and much of these are from routine, short trips that can be controlled by individual consumers. Because of this, sustainability advocates encourage greater use of alternative transportation modes such as mass transit and non-motorized transport to help limit carbon dioxide emissions. However, the efficacy of such prescriptions is contingent upon the social and physical context of a given place, that is, how these recommendations are received or put into practice by the intended audiences. This case study of Atlanta, Georgia's mostly African American Cascade community examines the influence of the broader social context of consumption as social practice and the built environment as factors influencing decisions about sustainable mobility (i.e., mass transit use and neighborhood walking), both inside and outside of Cascade. Not surprisingly, lower income residents routinely use mass transit, while middle-and upper-income earners are reluctant users of Atlanta's mass transit system (MARTA). Lack of use by those with higher incomes is due mainly to the availability of personal automobiles and inefficiencies in system design attributable to a history of racial politics that restricts MARTA to just two of metropolitan Atlanta's twenty-eight counties. Neighborhood walkability is encumbered by the lack of sidewalk space for higher income individuals and fear of crime for those with lower incomes. The social practice of status signaling via automobile purchasing may also inhibit African Americans' use of mass transit. C1 [Gaither, Cassandra Johnson; Schelhas, John] US Forest Serv, USDA, Washington, DC 20250 USA. [Himmelfarb, David] Eckerd Coll, St Petersburg, FL 33733 USA. [Hitchner, Sarah; Shepherd, J. Marshall] Univ Georgia, Athens, GA 30602 USA. [Binita, K. C.] Northeastern Univ, Boston, MA 02115 USA. RP Gaither, CJ (reprint author), US Forest Serv, USDA, Washington, DC 20250 USA. NR 47 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-0465 EI 1548-744X J9 CITY SOC JI City Soc. PD AUG PY 2016 VL 28 IS 2 BP 174 EP 197 DI 10.1111/ciso.12077 PG 24 WC Anthropology SC Anthropology GA DU8VZ UT WOS:000382495000008 ER PT J AU Jensen, G Schwarz, M Shumway, S Trushenski, J Woods, LC Broyles, T Mayeaux, M AF Jensen, Gary Schwarz, Michael Shumway, Sandra Trushenski, Jesse Woods, L. Curry, III Broyles, Thomas Mayeaux, Maxwell TI National Assessment Sheds Light on Educational Needs for Aquaculture in the United States SO FISHERIES LA English DT Article C1 [Jensen, Gary] 9324 Walking Horse Court, Springfield, VA 22153 USA. [Schwarz, Michael] Virginia Tech, Blacksburg, VA USA. [Shumway, Sandra] Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA. [Trushenski, Jesse] Idaho Dept Fish & Game, Eagle Fish Hlth Lab, Eagle, ID USA. [Woods, L. Curry, III] Univ Maryland, College Pk, MD 20742 USA. [Broyles, Thomas] Tennessee State Univ, Nashville, TN 37203 USA. [Mayeaux, Maxwell] Natl Inst Food & Agr, USDA, Washington, DC USA. RP Jensen, G (reprint author), 9324 Walking Horse Court, Springfield, VA 22153 USA. EM gljensen7@gmail.com NR 2 TC 0 Z9 0 U1 0 U2 0 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0363-2415 EI 1548-8446 J9 FISHERIES JI Fisheries PD AUG PY 2016 VL 41 IS 8 SI SI BP 467 EP 469 DI 10.1080/03632415.2016.1199830 PG 3 WC Fisheries SC Fisheries GA DU9PF UT WOS:000382549100011 ER PT J AU Ojwang, D Nyankanga, R Imungi, J Olanya, M Ukuku, D AF Ojwang, David Nyankanga, Richard Imungi, Japheth Olanya, Modesto Ukuku, Dike TI Cultivar preference and sensory evaluation of vegetable pigeon pea (Cajanus cajan) in Eastern Kenya SO FOOD SECURITY LA English DT Article DE Cajanus cajan; Pigeon pea; Cultivar acceptability; Sensory; Preference ID BEANS PHASEOLUS-VULGARIS AB Pigeon pea is an important crop in the dry regions of Eastern Kenya because of its drought tolerance and high protein content. Preference and acceptability of 12 vegetable pigeon pea cultivars of medium duration to maturity were evaluated by consumers and farmers in Eastern Kenya on the basis of six seed parameters: color, appearance, taste, aroma, tenderness and overall acceptability. They were scored on a hedonic scale of 1-7, where 1 = highly unfavorable to 7 = highly favorable. Significant differences (P < 0.05) in the six parameters were recorded, the cultivars ICEAP 00068, ICEAP 00540, ICEAP 00554, ICEAP 00902, KAT 60/8 and MZ 2/9 being preferred. As production of pigeon pea and other grain legumes increases, incorporation in selection processes of cultivar preferences/acceptability, organoleptic properties, post-harvest processing and utilization to preserve nutritional qualities could greatly improve adoption and therefore food security and livelihoods of resource-constrained farmers and consumers. Such a development would increase the consumption of pigeon pea and therefore enhance the nutrition intake and food security in the dry regions of Eastern Kenya. C1 [Ojwang, David] Solidaridad SECAEC, POB 42234-00100, Nairobi, Kenya. [Nyankanga, Richard] Univ Nairobi, Dept Plant Sci & Crop Protect, POB 30197-00100, Nairobi, Kenya. [Imungi, Japheth] Univ Nairobi, Dept Food Sci Nutr & Technol, POB 30197-00100, Nairobi, Kenya. [Olanya, Modesto; Ukuku, Dike] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Olanya, M (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM modesto.olanya@ars.usda.gov FU Solidaridad; University of Nairobi FX We acknowledge the funding support for this study from Solidaridad and the University of Nairobi. We are grateful for the technical support from USDA-Agricultural Research Service. NR 27 TC 0 Z9 0 U1 4 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1876-4517 EI 1876-4525 J9 FOOD SECUR JI Food Secur. PD AUG PY 2016 VL 8 IS 4 BP 757 EP 767 DI 10.1007/s12571-016-0592-8 PG 11 WC Food Science & Technology SC Food Science & Technology GA DV1TM UT WOS:000382704100006 ER PT J AU Neary, DG AF Neary, Daniel G. TI Long-Term Forest Paired Catchment Studies: What Do They Tell Us That Landscape-Level Monitoring Does Not? SO FORESTS LA English DT Article DE forest catchments; long-term studies; monitoring; water quantity; water quality ID STREAM-WATER-QUALITY; SITE PREPARATION; CENTRAL SWEDEN; RADIATA PINE; YIELD; MANAGEMENT; CHEMISTRY; CONVERSION; NITROGEN; AFFORESTATION AB Forested catchments throughout the world are known for producing high quality water for human use. In the 20th Century, experimental forest catchment studies played a key role in studying the processes contributing to high water quality. The hydrologic processes investigated on these paired catchments have provided the science base for examining water quality responses to natural disturbances such as wildfire, insect outbreaks, and extreme hydrologic events, and human-induced disturbances such as timber harvesting, site preparation, prescribed fires, fertilizer applications, pesticide usage, rainfall acidification, and mining. This paper compares and contrasts the paired catchment approach with landscape-level water resource monitoring to highlight the information on hydrologic processes provided by the paired catchment approach that is not provided by the broad-brush landscape monitoring. C1 [Neary, Daniel G.] USDA Forest Serv, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP Neary, DG (reprint author), USDA Forest Serv, Rocky Mt Res Stn, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM dneary@fs.fed.us FU USDA Forest Service Research and Development division FX The author would like to acknowledge the USDA Forest Service Research and Development division for providing support and training during his 38-year career in order to develop insights and experience in conducting paired watershed science. He would also like to thank SCION Forest Research Institute, Rotorua, New Zealand, for getting him into this aspect of the hydrological sciences. NR 88 TC 0 Z9 0 U1 3 U2 3 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD AUG PY 2016 VL 7 IS 8 AR 164 DI 10.3390/f7080164 PG 15 WC Forestry SC Forestry GA DU8NI UT WOS:000382470000013 ER PT J AU Ritokova, G Shaw, DC Filip, G Kanaskie, A Browning, J Norlander, D AF Ritokova, Gabriela Shaw, David C. Filip, Greg Kanaskie, Alan Browning, John Norlander, Danny TI Swiss Needle Cast in Western Oregon Douglas-Fir Plantations: 20-Year Monitoring Results SO FORESTS LA English DT Article DE Douglas-fir; foliage disease; Swiss Needle Cast; forest pathology; disease severity assessment; aerial survey ID PHAEOCRYPTOPUS-GAEUMANNII; FOLIAGE RETENTION; COASTAL OREGON; SEVERITY; GROWTH; STANDS; RANGE; AREA AB Swiss needle cast (SNC), a foliar disease specific to Douglas-fir (Pseudotsuga menziesii), is caused by an endemic Ascomycete fungus (Phaeocryptopus gaeumannii). In the late 1980s and early 1990s significant symptoms began to appear in coastal Oregon, and these have been associated with the planting of Douglas-fir in the Sitka spruce zone, leaf wetness during potential spore dispersal in May-August, and mild winter temperature. The first annual aerial survey was initiated in 1996 and has continued through 2015, which indicates a significant increase in area of visible symptoms from the air, increasing from 53,050 ha in 1996 to 238,705 ha in 2015. Monitoring plots in the NW Oregon Coast Range verified impacts of SNC on tree growth and productivity, with growth reductions averaging about 23% in the epidemic area linked to needle retention. A series of monitoring plots was set up in the western Cascade Mountains of Oregon and 590 10-23-year old Douglas-fir trees in 59 stands were tracked for 10 years, measured in 2001, 2006, and 2011. No measureable growth impacts were noted in this region of Oregon. A new plot network is being installed throughout the Oregon and southwest Washington coastal ranges as a means of monitoring future disease impact and providing framework for additional studies. C1 [Ritokova, Gabriela; Shaw, David C.] Oregon State Univ, Dept Forest Engn Resources & Management, 280 Peavy Hall, Corvallis, OR 97331 USA. [Filip, Greg] US Forest Serv, 1220 SW Third Ave, Portland, OR 97208 USA. [Kanaskie, Alan; Norlander, Danny] Oregon Dept Forestry, 2600 State St, Salem, OR 97310 USA. [Browning, John] Weyerhaeuser Corp, 505 N Pearl St, Centralia, WA 98531 USA. RP Ritokova, G (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, 280 Peavy Hall, Corvallis, OR 97331 USA. EM gabriela.ritokova@oregonstate.edu; dave.shaw@oregonstate.edu; gmfilip@fs.fed.us; alan.kanaskie@oregon.gov; john.browning@weyerhaeuser.com; danny.norlander@oregon.gov FU US Forest Service, Forest Health Monitoring Program, Oregon Department of Forestry; Swiss Needle Cast Cooperative FX Funding for the Cooperative Aerial Survey was from US Forest Service, Forest Health Monitoring Program, Oregon Department of Forestry, and Swiss Needle Cast Cooperative membership. We thank Mike McWilliams, Floyd Freeman, United States Department of Agriculture, Forest Service; and Bob Ohrn and Charlie Thompson, Salem Bureau of Land Management. We also thank Jon Laine, Kevin Nelson, and Michael Thompson from ODF and field crews from the Salem and Eugene BLM and the Mt. Hood and Willamette NF's for data collection; and J.T. Marrs Contract Cutting for tree climbing and sampling. We are grateful to Robbie Flowers, USDA Forest Service for contributing the aerial photograph of mixed species stand. NR 32 TC 0 Z9 0 U1 9 U2 9 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD AUG PY 2016 VL 7 IS 8 AR 155 DI 10.3390/f7080155 PG 11 WC Forestry SC Forestry GA DU8NI UT WOS:000382470000004 ER PT J AU Zalamea, M Gonzalez, G Lodge, DJ AF Zalamea, Marcela Gonzalez, Grizelle Lodge, Deborah Jean TI Physical, Chemical, and Biological Properties of Soil under Decaying Wood in a Tropical Wet Forest in Puerto Rico SO FORESTS LA English DT Article DE coarse woody debris (CWD); wood decomposition; soil properties; available nutrients; roots; microbial biomas; tropical forest; Puerto Rico ID LUQUILLO EXPERIMENTAL FOREST; ORGANIC-MATTER DYNAMICS; CONIFEROUS FOREST; CYRILLA-RACEMIFLORA; MICROBIAL BIOMASS; RAIN-FOREST; FIR FOREST; BOLE-WOOD; NITROGEN; DEBRIS AB Decaying wood is related to nutrient cycling through its role as either a sink or source of nutrients. However, at micro scales, what is the effect of decaying logs on the physical, chemical, and biotic characteristics of the soil underneath? We took samples from a 0 to 5 cm depth under and a 50 cm distance away from decaying logs (Dacryodes excelsa and Swietenia macrophylla) at 2 stages of decay, and measured soil temperature, total and available nutrients, and root length in a tropical wet forest. We found decaying wood affected physical, chemical, and biotic properties of the underlying soil. Soil temperature was less variable under the decaying logs than away from the logs. Soil under the decaying wood had fewer roots, and lower NO3- and Mg2+ availability than samples collected a distance of 50 cm away from the logs. Tree species and decay stage were important factors defining the effect of decaying wood on the distribution of available nutrients. Ca2+, Mg2+, and K+ levels were higher in the soil associated with the youngest logs, and were higher near S. macrophylla logs. Heavy metals were also higher in the soil located near the younger logs independent of the species; other metal ions such as Al3+ and Fe3+ were higher in the soil associated with D. excelsa and the oldest logs. These results indicate decaying wood can contribute to and generate spatial heterogeneity of soil properties. C1 [Zalamea, Marcela; Gonzalez, Grizelle] US Forest Serv, USDA, Int Inst Trop Forestry, Jardin Bot Sur,1201 Ceiba St Rio Piedras, San Juan, PR 00926 USA. [Lodge, Deborah Jean] US Forest Serv, USDA, No Res Stn, Jardin Bot Sur,1201 Ceiba St Rio Piedras, Luquillo, PR 00773 USA. RP Gonzalez, G (reprint author), US Forest Serv, USDA, Int Inst Trop Forestry, Jardin Bot Sur,1201 Ceiba St Rio Piedras, San Juan, PR 00926 USA. EM ggonzalez@fs.fed.us; dlodge@fs.fed.us FU National Science Foundation [DEB-0218039, 1239764]; United States Department of Agriculture, Forest Service, International Institute of Tropical Forestry; Luquillo Critical Zone Observatory [EAR-1331841]; Forest Service (U.S. Department of Agriculture); CREST-Center for Applied Tropical Ecology and Conservation; Biology Graduate Program at the University of Puerto Rico; Organization for Tropical Studies and Conservation (OTS) FX This research was performed under grants DEB-0218039 and 1239764 from the National Science Foundation to the Institute of Tropical Ecosystem Studies, University of Puerto Rico, and the United States Department of Agriculture, Forest Service, International Institute of Tropical Forestry as part of the Long-Term Ecological Research Program in the Luquillo Experimental Forest. Additional support was provided by the Luquillo Critical Zone Observatory (EAR-1331841), the Forest Service (U.S. Department of Agriculture), CREST-Center for Applied Tropical Ecology and Conservation, the Biology Graduate Program at the University of Puerto Rico, and the Organization for Tropical Studies and Conservation (OTS). We are thankful to Samuel Moya and Carlos Estrada who helped locate and identify logs; Maria M. Rivera, Mary Jeane Sanchez, Maysaa Ittayem, Edwin Lopez, Carmen Marrero, Maribelis Santiago, and other staff at the International Institute of Tropical Forestry-Soil Laboratory who helped process soil samples; Veronica Cruz and Jesus Santiago who helped with microbial biomass estimation and root separation; Andres Fernandez who helped grind soil samples; Ligia Lebron for methodological guidance; Maya Quinones and Olga Ramos helped with Figure 1. Elvira Cuevas from the University of Puerto Rico kindly provided the Delta-T scanner and software, and her advice was very helpful to Marcela Zalamea. Ryan Hangs from Western Ag Innovations and Jeff Schoenau from University of Saskatchewan-Saskatoon, Canada, provided helpful comments on the discussion section, while Juan C. Bello Edmund Tanner, and Ariel E. Lugo commented on an earlier version of the manuscript. NR 77 TC 1 Z9 1 U1 11 U2 11 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD AUG PY 2016 VL 7 IS 8 AR 168 DI 10.3390/f7080168 PG 18 WC Forestry SC Forestry GA DU8NI UT WOS:000382470000017 ER PT J AU Goodwin, SB Mccorison, CB Cavaletto, JR Culley, DE Labutti, K Baker, SE Grigoriev, IV AF Goodwin, Stephen B. Mccorison, Cassandra B. Cavaletto, Jessica R. Culley, David E. Labutti, Kurt Baker, Scott E. Grigoriev, Igor V. TI The mitochondrial genome of the ethanol-metabolizing, wine cellar mold Zasmidium cellare is the smallest for a filamentous ascomycete SO FUNGAL BIOLOGY LA English DT Article DE Filamentous; Fungus; Mitochondrial genome; Smallest; Synteny; Zasmidium cellare ID YEAST MITOCHONDRIA; DNA-SEQUENCES; ORGANIZATION; EVOLUTION; FUNGI; DIVERGENCE; RACODIUM AB Fungi in the class Dothideomycetes often live in extreme environments or have unusual physiology. One of these, the wine cellar mold Zasmidium cellare, produces thick curtains of mycelia in cellars with high humidity, and its ability to metabolize volatile organic compounds is thought to improve air quality. Whether these abilities have affected its mitochondrial genome is not known. To fill this gap, the circular-mapping mitochondria' genome of Z. cellare was sequenced and, at only 23 743 bp, is the smallest reported for a filamentous fungus. Genes were encoded on both strands with a single change of direction, different from most other fungi but consistent with the Dothideomycetes. Other than its small size, the only unusual feature of the Z. cellare mitochondrial genome was two copies of a 110-bp sequence that were duplicated, inverted and separated by approximately 1 kb. This inverted-repeat sequence confused the assembly program but appears to have no functional significance. The small size of the Z. cellare mitochondrial genome was due to slightly smaller genes, lack of introns and non-essential genes, reduced intergenic spacers and very few ORFs relative to other fungi rather than a loss of essential genes. Whether this reduction facilitates its unusual biology remains unknown. Published by Elsevier Ltd on behalf of British Mycological Society. C1 [Goodwin, Stephen B.; Cavaletto, Jessica R.] Purdue Univ, ARS, USDA, Crop Prod & Pest Control Res Unit, 915 West State St, W Lafayette, IN 47907 USA. [Mccorison, Cassandra B.] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Culley, David E.] Pacific Northwest Natl Lab, Chem & Biol Proc Dev Grp, 902 Battelle Blvd,POB 999,MSIN P8-60, Richland, WA 99352 USA. [Labutti, Kurt; Grigoriev, Igor V.] US DOE, Joint Genome Inst, 2800 Mitchell Dr, Walnut Creek, CA 94598 USA. [Baker, Scott E.] Pacific Northwest Natl Lab, Environm Mol Sci Lab, 3335 Innovat Blvd, Richland, WA 99354 USA. RP Goodwin, SB (reprint author), Purdue Univ, ARS, USDA, Crop Prod & Pest Control Res Unit, 915 West State St, W Lafayette, IN 47907 USA. EM sgoodwin@purdue.edu FU USDA-ARS CRIS project [3602-22000-017-00D]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231] FX We thank Stephenie Droll for translating references from German into English and Rachel Koch for help with the phylogeny. This work was funded by USDA-ARS CRIS project 3602-22000-017-00D. Sequencing of the Z. cellare mitochondrial genome was performed at the U. S. Department of Energy's Joint Genome Institute through the Community Sequencing Program (www.jgi.doe.govicsp/) and all sequence data are publicly available. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. NR 47 TC 0 Z9 0 U1 2 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD AUG PY 2016 VL 120 IS 8 BP 961 EP 974 DI 10.1016/j.funbio.2016.05.003 PG 14 WC Mycology SC Mycology GA DU6QX UT WOS:000382340800006 PM 27521628 ER PT J AU Bulli, P Zhang, JL Chao, SM Chen, XM Pumphrey, M AF Bulli, Peter Zhang, Junli Chao, Shiaoman Chen, Xianming Pumphrey, Michael TI Genetic Architecture of Resistance to Stripe Rust in a Global Winter Wheat Germplasm Collection SO G3-GENES GENOMES GENETICS LA English DT Article DE hexaploid wheat; disease resistance; yellow rust; association mapping; QTL-tag SNP; genetics of immunity ID F-SP TRITICI; ADULT-PLANT RESISTANCE; GENOME-WIDE ASSOCIATION; BULKED SEGREGANT ANALYSIS; QUANTITATIVE TRAIT LOCI; PUCCINIA-STRIIFORMIS; SPRING WHEAT; LINKAGE DISEQUILIBRIUM; UNITED-STATES; AESTIVUM L. AB Virulence shifts in populations of Puccinia striiformis f. sp. tritici (Pst), the causal pathogen of wheat stripe rust, are a major challenge to resistance breeding. The majority of known resistance genes are already ineffective against current races of Pst, necessitating the identification and introgression of new sources of resistance. Germplasm core collections that reflect the range of genetic and phenotypic diversity of crop species are ideal platforms for examining the genetic architecture of complex traits such as resistance to stripe rust. We report the results of genetic characterization and genome-wide association analysis (GWAS) for resistance to stripe rust in a core subset of 1175 accessions in the National Small Grains Collection (NSGC) winter wheat germplasm collection, based on genotyping with the wheat 9K single nucleotide polymorphism (SNP) iSelect assay and phenotyping of seedling and adult plants under natural disease epidemics in four environments. High correlations among the field data translated into high heritability values within and across locations. Population structure was evident when accessions were grouped by stripe rust reaction. GWAS identified 127 resistance loci that were effective across at least two environments, including 20 with significant genome-wide adjusted P-values. Based on relative map positions of previously reported genes and QTL, five of the QTL with significant genome-wide adjusted P-values in this study represent potentially new loci. This study provides an overview of the diversity of Pst resistance in the NSGC winter wheat germplasm core collection, which can be exploited for diversification of stripe rust resistance in breeding programs. C1 [Bulli, Peter; Pumphrey, Michael] Washington State Univ, Dept Crop & Soil Sci, 381 Johnson Hall,POB 646420, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, USDA ARS, Wheat Hlth Genet & Qual Res Unit, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Zhang, Junli] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Chao, Shiaoman] ARS, USDA, Genotyping Lab, Biosci Res Lab, Fargo, ND 58102 USA. RP Pumphrey, M (reprint author), Washington State Univ, Dept Crop & Soil Sci, 381 Johnson Hall,POB 646420, Pullman, WA 99164 USA. EM m.pumphrey@wsu.edu OI Bulli, Peter/0000-0002-7223-7838 FU National Research Initiative Competitive Grant (Triticeae-CAP) from the United States Department of Agriculture National Institute of Food and Agriculture [2011-68002-30029]; Washington Wheat Commission FX Sheri Rynearson, John Kuehner, Victor DeMacon, Dr. Anmin Wan, Dr. Meinan Wang, and Dr. Kent Evans are appreciated for providing technical support. This project was supported by the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the United States Department of Agriculture National Institute of Food and Agriculture and the Washington Wheat Commission. The funders had no role in the study design, data collection, decision to publish, or preparation of the manuscript. Author contributions: P.B. performed the field experiments, data analysis, and drafted the manuscript. J.Z. contributed to data analysis and manuscript revision. S.C. performed the genotyping experiment and revised the manuscript. X.C. contributed to the design and supervision of the field experiment and revised the manuscript. M.P. supervised the experiments from start to finish and critically revised the manuscript. All authors have read and approved the final version of the manuscript. The authors declare that they have no competing interests. NR 106 TC 1 Z9 1 U1 21 U2 21 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD AUG 1 PY 2016 VL 6 IS 8 BP 2237 EP 2253 DI 10.1534/g3.116.028407/-/DC1 PG 17 WC Genetics & Heredity SC Genetics & Heredity GA DT2AD UT WOS:000381282300001 PM 27226168 ER PT J AU Jia, SG Li, AX Morton, K Avoles-Kianian, P Kianian, SF Zhang, C Holding, D AF Jia, Shangang Li, Aixia Morton, Kyla Avoles-Kianian, Penny Kianian, Shahryar F. Zhang, Chi Holding, David TI A Population of Deletion Mutants and an Integrated Mapping and Exome-seq Pipeline for Gene Discovery in Maize SO G3-GENES GENOMES GENETICS LA English DT Article DE maize functional genomics; BSR-seq; exome-seq; opaque; causative mutation ID QUALITY PROTEIN MAIZE; SEQUENCING DATA; WILD-TYPE; RNA-SEQ; MUTATION IDENTIFICATION; ENDOSPERM MODIFICATION; EXPRESSION ANALYSIS; REVERSE GENETICS; CLONAL ANALYSIS; READ ALIGNMENT AB To better understand maize endosperm filling and maturation, we used gamma-irradiation of the B73 maize reference line to generate mutants with opaque endosperm and reduced kernel fill phenotypes, and created a population of 1788 lines including 39 Mo17 x F2s showing stable, segregating, and viable kernel phenotypes. For molecular characterization of the mutants, we developed a novel functional genomics platform that combined bulked segregant RNA and exome sequencing (BSREx-seq) to map causative mutations and identify candidate genes within mapping intervals. To exemplify the utility of the mutants and provide proof-of-concept for the bioinformatics platform, we present detailed characterization of line 937, an opaque mutant harboring a 6203 bp in-frame deletion covering six exons within the Opaque-1 gene. In addition, we describe mutant line 146 which contains a 4.8 kb intragene deletion within the Sugary-1 gene and line 916 in which an 8.6 kb deletion knocks out a Cyclin A2 gene. The publically available algorithm developed in this work improves the identification of causative deletions and its corresponding gaps within mapping peaks. This study demonstrates the utility of gamma-irradiation for forward genetics in large nondense genomes such as maize since deletions often affect single genes. Furthermore, we show how this classical mutagenesis method becomes applicable for functional genomics when combined with state-of-the-art genomics tools. C1 [Jia, Shangang; Li, Aixia; Morton, Kyla; Holding, David] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68588 USA. [Zhang, Chi] Univ Nebraska, Sch Biol Sci, Ctr Plant Sci Innovat, Beadle Ctr Biotechnol, Lincoln, NE 68588 USA. [Avoles-Kianian, Penny; Kianian, Shahryar F.] Univ Minnesota, USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Holding, D (reprint author), E323 Beadle Ctr Biotechnol, 1901 Vine St,POB 880665, Lincoln, NE 68588 USA. EM dholding2@unl.edu FU Holland Computing Center of the University of Nebraska; UNL Center for Plant Science Innovation Program of Excellence; UNL Department of Agronomy and Horticulture; Agriculture and Food Research Initiative competitive of the United States Department of Agriculture National Institute of Food and Agriculture [2013-02278] FX The authors thank Patrick Schnable and his laboratory for designing the Maize_ISU_RNG_Zeaome_EZ_HX1 SeqCap EZ array and allowing its use prior to publication. The authors thank Rentao Song for the gift of the O1 antibody. The Hiseq sequencing and exome-capture were performed by the University of Minnesota Genomics Center. The authors thank Lingling Yuan for early assistance with field work and Ying Ren for useful discussions. The computing work in this project was supported by the Holland Computing Center of the University of Nebraska. This work was supported by the UNL Center for Plant Science Innovation Program of Excellence and the UNL Department of Agronomy and Horticulture and by the Agriculture and Food Research Initiative competitive grant no. 2013-02278 of the United States Department of Agriculture National Institute of Food and Agriculture. NR 57 TC 0 Z9 0 U1 7 U2 7 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD AUG 1 PY 2016 VL 6 IS 8 BP 2385 EP 2395 DI 10.1534/g3.116.030528/-/DC1 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA DT2AD UT WOS:000381282300014 PM 27261000 ER PT J AU Morota, G Beissinger, TM Penagaricano, F AF Morota, Gota Beissinger, Timothy M. Penagaricano, Francisco TI MeSH-Informed Enrichment Analysis and MeSH-Guided Semantic Similarity Among Functional Terms and Gene Products in Chicken SO G3-GENES GENOMES GENETICS LA English DT Article DE annotation; chicken; enrichment analysis; MeSH; semantic similarity ID LINKAGE DISEQUILIBRIUM; GO; ASSOCIATION; SELECTION; ONTOLOGY; TOOL AB Biomedical vocabularies and ontologies aid in recapitulating biological knowledge. The annotation of gene products is mainly accelerated by Gene Ontology (GO), and more recently by Medical Subject Headings (MeSH). Here, we report a suite of MeSH packages for chicken in Bioconductor, and illustrate some features of different MeSH-based analyses, including MeSH-informed enrichment analysis and MeSH-guided semantic similarity among terms and gene products, using two lists of chicken genes available in public repositories. The two published datasets that were employed represent (i) differentially expressed genes, and (ii) candidate genes under selective sweep or epistatic selection. The comparison of MeSH with GO overrepresentation analyses suggested not only that MeSH supports the findings obtained from GO analysis, but also that MeSH is able to further enrich the representation of biological knowledge and often provide more interpretable results. Based on the hierarchical structures of MeSH and GO, we computed semantic similarities among vocabularies, as well as semantic similarities among selected genes. These yielded the similarity levels between significant functional terms, and the annotation of each gene yielded the measures of gene similarity. Our findings show the benefits of using MeSH as an alternative choice of annotation in order to draw biological inferences from a list of genes of interest. We argue that the use of MeSH in conjunction with GO will be instrumental in facilitating the understanding of the genetic basis of complex traits. C1 [Morota, Gota] Univ Nebraska, Dept Anim Sci, POB 830908, Lincoln, NE 68583 USA. [Beissinger, Timothy M.] Univ Missouri, ARS, USDA, Columbia, MO 65211 USA. [Beissinger, Timothy M.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Penagaricano, Francisco] Univ Florida, Dept Anim Sci, Gainesville, FL 32610 USA. [Penagaricano, Francisco] Univ Florida, Genet Inst, Gainesville, FL 32610 USA. RP Morota, G (reprint author), Univ Nebraska, Dept Anim Sci, POB 830908, Lincoln, NE 68583 USA. EM morota@unl.edu FU University of Nebraska Layman Fund; United States Department of Agriculture-Agricultural Research Service; Florida Agricultural Experiment Station; Department of Animal Sciences, University of Florida FX This work was supported in part by the University of Nebraska Layman Fund to G.M. Support for T.M.B. was provided by the United States Department of Agriculture-Agricultural Research Service. F.P. acknowledges financial support from the Florida Agricultural Experiment Station and the Department of Animal Sciences, University of Florida. NR 20 TC 1 Z9 1 U1 3 U2 3 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD AUG 1 PY 2016 VL 6 IS 8 BP 2447 EP 2453 DI 10.1534/g3.116.031096/-/DC1 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA DT2AD UT WOS:000381282300019 PM 27261003 ER PT J AU Liu, ZB Garcia, A McMullen, MD Flint-Garcia, SA AF Liu, Zhengbin Garcia, Arturo McMullen, Michael D. Flint-Garcia, Sherry A. TI Genetic Analysis of Kernel Traits in Maize-Teosinte Introgression Populations SO G3-GENES GENOMES GENETICS LA English DT Article DE introgression population; kernel weight; kernel size and shape; quantitative trait loci (QTL); domestication; Multiparent Advanced Generation Inter-Cross (MAGIC); multiparental populations; MPP ID ASSOCIATION MAPPING POPULATION; RICE GRAIN WIDTH; SEED SIZE; RARE ALLELE; MAJOR QTL; FRUIT SIZE; DOMESTICATION; EVOLUTION; WEIGHT; YIELD AB Seed traits have been targeted by human selection during the domestication of crop species as a way to increase the caloric and nutritional content of food during the transition from hunter-gather to early farming societies. The primary seed trait under selection was likely seed size/weight as it is most directly related to overall grain yield. Additional seed traits involved in seed shape may have also contributed to larger grain. Maize (Zea mays ssp. mays) kernel weight has increased more than 10-fold in the 9000 years since domestication from its wild ancestor, teosinte (Z. mays ssp. parviglumis). In order to study how size and shape affect kernel weight, we analyzed kernel morphometric traits in a set of 10 maize-teosinte introgression populations using digital imaging software. We identified quantitative trait loci (QTL) for kernel area and length with moderate allelic effects that colocalize with kernel weight QTL. Several genomic regions with strong effects during maize domestication were detected, and a genetic framework for kernel traits was characterized by complex pleiotropic interactions. Our results both confirm prior reports of kernel domestication loci and identify previously uncharacterized QTL with a range of allelic effects, enabling future research into the genetic basis of these traits. C1 [Liu, Zhengbin; McMullen, Michael D.; Flint-Garcia, Sherry A.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Garcia, Arturo; McMullen, Michael D.; Flint-Garcia, Sherry A.] Univ Missouri, ARS, USDA, Columbia, MO 65211 USA. [Liu, Zhengbin] Danforth Ctr, St Louis, MO 63132 USA. RP Flint-Garcia, SA (reprint author), Univ Missouri, 301 Curtis Hall, Columbia, MO 65211 USA. EM Sherry.Flint-Garcia@ars.usda.gov FU National Science Foundation [IOS-0820619]; United States Department of Agriculture (USDA)-Agriculture Research Service (ARS) FX We wish to thank AgReliant for the production of the doubled haploid lines from the Z031 population, and the McMullen, Flint-Garcia, and Buckler groups for growing the trials, data collection, and providing ear and seed samples. We also thank the anonymous reviewers for their helpful comments on the manuscript. This research was supported by the National Science Foundation (IOS-0820619) and United States Department of Agriculture (USDA)-Agriculture Research Service (ARS). Mention of a trademark, vendor, or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. The USDA-ARS, Midwest Area, is an equal opportunity, affirmative action employer, and all agency services are available without discrimination. NR 63 TC 1 Z9 1 U1 13 U2 13 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD AUG 1 PY 2016 VL 6 IS 8 BP 2523 EP 2530 DI 10.1534/g3.116.030155/-/DC1 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA DT2AD UT WOS:000381282300026 PM 27317774 ER PT J AU Pyle, J Patel, T Merrill, B Nsokoshi, C McCall, M Proctor, RH Brown, DW Hammond, TM AF Pyle, Jay Patel, Tejas Merrill, Brianna Nsokoshi, Chabu McCall, Morgan Proctor, Robert H. Brown, Daren W. Hammond, Thomas M. TI A Meiotic Drive Element in the Maize Pathogen Fusarium verticillioides Is Located Within a 102 kb Region of Chromosome V SO G3-GENES GENOMES GENETICS LA English DT Article DE genomic conflict; mapping; meiotic drive; fungi; spore killing ID HET-S PRION; GIBBERELLA-FUJIKUROI; SPORE KILLER; HETEROKARYON INCOMPATIBILITY; PODOSPORA-ANSERINA; MONILIFORME; NEUROSPORA; FUMONISINS; GENERATION; ALIGNMENT AB Fusarium verticillioides is an agriculturally important fungus because of its association with maize and its propensity to contaminate grain with toxic compounds. Some isolates of the fungus harbor a meiotic drive element known as Spore killer (Sk(K)) that causes nearly all surviving meiotic progeny from an Sk(K) x Spore killer-susceptible (Sk(S)) cross to inherit the Sk(K) allele. Sk(K) has been mapped to chromosome V but the genetic element responsible for meiotic drive has yet to be identified. In this study, we used cleaved amplified polymorphic sequence markers to genotype individual progeny from an Sk(K) x Sk(S) mapping population. We also sequenced the genomes of three progeny from the mapping population to determine their single nucleotide polymorphisms. These techniques allowed us to refine the location of Sk(K) to a contiguous 102 kb interval of chromosome V, herein referred to as the Sk region. Relative to Sk(S) genotypes, Sk(K) genotypes have one extra gene within this region for a total of 42 genes. The additional gene in Sk(K) genotypes, herein named SKC1 for Spore Killer Candidate 1, is the most highly expressed gene from the Sk region during early stages of sexual development. The Sk region also has three hyper-variable regions, the longest of which includes SKC1. The possibility that SKC1, or another gene from the Sk region, is an essential component of meiotic drive and spore killing is discussed. C1 [Pyle, Jay; Patel, Tejas; Merrill, Brianna; Nsokoshi, Chabu; McCall, Morgan; Hammond, Thomas M.] Illinois State Univ, Sch Biol Sci, Julian Hall 210,Campus Box 4120, Normal, IL 61790 USA. [Proctor, Robert H.; Brown, Daren W.] ARS, Mycotoxin Prevent & Appl Microbiol, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Hammond, TM (reprint author), Illinois State Univ, Sch Biol Sci, Julian Hall 210,Campus Box 4120, Normal, IL 61790 USA.; Brown, DW (reprint author), Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM daren.brown@ars.usda.gov; tmhammo@ilstu.edu FU Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) of the National Institutes of Health [1R15HD076309-01] FX Dr. John Leslie (Kansas State University) and Dr. Frances Trail (Michigan State University) kindly donated strains Fv999-SkK and Fv149-SkS. We thank the Fungal Genetics Stock Center (McCluskey et al. 2010) for other key strains and the Broad Institute of Harvard and the Massachusetts Institute of Technology (www.broad.mit.edu) for access to the Fusarium Comparative Database. Chris McGovern and Nathane Orwig of the National Center for Agricultural Utilization Research provided much appreciated technical assistance. We thank Dr. Sven Saupe (Universite de Bordeaux) for sharing his knowledge of the het-s spore killing mechanism. T.M.H. was supported by a grant from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) of the National Institutes of Health (1R15HD076309-01). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 55 TC 0 Z9 0 U1 3 U2 3 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD AUG 1 PY 2016 VL 6 IS 8 BP 2543 EP 2552 DI 10.1534/g3.116.029728/-/DC1 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA DT2AD UT WOS:000381282300028 PM 27317777 ER PT J AU Baranowski, T AF Baranowski, Tom TI Types of Articles for Publishing Your G4H SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material ID RANDOMIZED CONTROLLED-TRIAL; PHYSICAL-ACTIVITY; VEGETABLE CONSUMPTION; SERIOUS VIDEOGAME; DIETARY; PILOT; GAME; FEASIBILITY; FEEDBACK; DESIGN C1 [Baranowski, Tom] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu NR 20 TC 0 Z9 0 U1 2 U2 2 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD AUG PY 2016 VL 5 IS 4 BP 237 EP 240 DI 10.1089/g4h.2016.0039 PG 4 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA DV0LU UT WOS:000382609800001 PM 27427972 ER PT J AU Shi, AN Mou, BQ AF Shi, Ainong Mou, Beiquan TI Genetic diversity and association analysis of leafminer (Liriomyza langei) resistance in spinach (Spinacia oleracea) SO GENOME LA English DT Article DE association mapping; genotyping by sequencing; leafminer; single nucleotide polymorphism; spinach; Spinacia oleracea ID MARKER-ASSISTED-SELECTION; AGROMYZIDAE; DIPTERA; IDENTIFICATION; SOFTWARE; LETTUCE; TOOL AB Leafminer (Liriomyza langei) is a major insect pest of many important agricultural crops, including spinach (Spinacia oleracea). Use of genetic resistance is an efficient, economic, and environment-friendly method to control this pest. The objective of this research was to conduct association analysis and identify single nucleotide polymorphism (SNP) markers associated with leafminer resistance in spinach germplasm. A total of 300 USDA spinach germplasm accessions were used for the association analysis of leafminer resistance. Genotyping by sequencing (GBS) was used for genotyping and 783 SNPs from GBS were used for association analysis. The leafminer resistance showed a near normal distribution with a wide range from 1.1 to 11.7 stings per square centimeter leaf area, suggesting that the leafminer resistance in spinach is a complex trait controlled by multiple genes with minor effect in this spinach panel. Association analysis indicated that five SNP markers, AYZV02040968_7171, AYZV02076752_412, AYZV02098618_4615, AYZV02147304_383, and AYZV02271373_398, were associated with the leafminer resistance with LOD 2.5 or higher. The SNP markers may be useful for breeders to select plants and lines for leafminer resistance in spinach breeding programs through marker-assisted selection. C1 [Shi, Ainong] Univ Arkansas, Dept Hort, 316 PTSC, Fayetteville, AR 72701 USA. [Mou, Beiquan] ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. RP Shi, AN (reprint author), Univ Arkansas, Dept Hort, 316 PTSC, Fayetteville, AR 72701 USA.; Mou, BQ (reprint author), ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. EM ashi@uark.edu; Beiquan.Mou@ARS.USDA.GOV FU USDA National Institute of Food and Agriculture [1002423] FX This work is supported, in part, by the USDA National Institute of Food and Agriculture Hatch project accession number 1002423. NR 42 TC 0 Z9 0 U1 10 U2 10 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD AUG PY 2016 VL 59 IS 8 BP 581 EP 588 DI 10.1139/gen-2016-0075 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA DT1FV UT WOS:000381228000006 PM 27490441 ER PT J AU Shwab, EK Jiang, TT Pena, HFJ Gennari, SM Dubey, JP Su, CL AF Shwab, Elliot Keats Jiang, Tiantian Pena, Hilda F. J. Gennari, Solange M. Dubey, Jitender P. Su, Chunlei TI The ROP18 and ROP5 gene allele types are highly predictive of virulence in mice across globally distributed strains of Toxoplasma gondii (vol 46, pg 141, 2016) SO INTERNATIONAL JOURNAL FOR PARASITOLOGY LA English DT Correction C1 [Shwab, Elliot Keats; Jiang, Tiantian; Su, Chunlei] Univ Tennessee, Dept Microbiol, 1414 W Cumberland Ave, Knoxville, TN 37996 USA. [Pena, Hilda F. J.; Gennari, Solange M.] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Ave Prof Doutor Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP, Brazil. [Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Su, CL (reprint author), Univ Tennessee, Dept Microbiol, 1414 W Cumberland Ave, Knoxville, TN 37996 USA. EM csu1@utk.edu NR 1 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0020-7519 EI 1879-0135 J9 INT J PARASITOL JI Int. J. Parasit. PD AUG PY 2016 VL 46 IS 9 BP 603 EP 603 DI 10.1016/j.ijpara.2016.05.003 PG 1 WC Parasitology SC Parasitology GA DT5QP UT WOS:000381538000008 ER PT J AU Del Gobbo, LC Imamura, F Aslibekyan, S Marklund, M Virtanen, JK Wennberg, M Yakoob, MY Chiuve, SE dela Cruz, L Frazier-Wood, AC Fretts, AM Guallar, E Matsumoto, C Prem, K Tanaka, T Wu, JHY Zhou, X Helmer, C Ingelsson, E Yuan, JM Barberger-Gateau, P Campos, H Chaves, PHM Djousse, L Giles, GG Gomez-Aracena, J Hodge, AM Hu, FB Jansson, JH Johansson, I Khaw, KT Koh, WP Lemaitre, RN Lind, L Luben, RN Rimm, EB Riserus, U Samieri, C Franks, PW Siscovick, DS Stampfer, M Steffen, LM Steffen, BT Tsai, MY van Dam, RM Voutilainen, S Willett, WC Woodward, M Mozaffarian, D AF Del Gobbo, Liana C. Imamura, Fumiaki Aslibekyan, Stella Marklund, Matti Virtanen, Jyrki K. Wennberg, Maria Yakoob, Mohammad Y. Chiuve, Stephanie E. dela Cruz, Luicito Frazier-Wood, Alexis C. Fretts, Amanda M. Guallar, Eliseo Matsumoto, Chisa Prem, Kiesha Tanaka, Tosh Wu, Jason H. Y. Zhou, Xia Helmer, Catherine Ingelsson, Erik Yuan, Jian-Min Barberger-Gateau, Pascale Campos, Hannia Chaves, Paulo H. M. Djousse, Luc Giles, Graham G. Gomez-Aracena, Jose Hodge, Allison M. Hu, Frank B. Jansson, Jan-Hakan Johansson, Ingegerd Khaw, Kay-Tee Koh, Woon-Puay Lemaitre, Rozenn N. Lind, Lars Luben, Robert N. Rimm, Eric B. Riserus, Ulf Samieri, Cecilia Franks, Paul W. Siscovick, David S. Stampfer, Meir Steffen, Lyn M. Steffen, Brian T. Tsai, Michael Y. van Dam, Rob M. Voutilainen, Sari Willett, Walter C. Woodward, Mark Mozaffarian, Dariush CA Cohorts Heart Aging Res Genomic Ep Fatty Acids Outcomes Res Consortiu TI omega-3 Polyunsaturated Fatty Acid Biomarkers and Coronary Heart Disease Pooling Project of 19 Cohort Studies SO JAMA INTERNAL MEDICINE LA English DT Article ID ALPHA-LINOLENIC ACID; LONG-CHAIN N-3; CARDIOVASCULAR-DISEASE; CONTROLLED-TRIALS; FISH CONSUMPTION; ADIPOSE-TISSUE; BREAST-CANCER; DOSE-RESPONSE; RISK-FACTORS; DIETARY AB IMPORTANCE The role of omega-3 polyunsaturated fatty acids for primary prevention of coronary heart disease (CHD) remains controversial. Most prior longitudinal studies evaluated self-reported consumption rather than biomarkers. OBJECTIVE To evaluate biomarkers of seafood-derived eicosapentaenoic acid (EPA; 20: 5 omega-3), docosapentaenoic acid (DPA; 22: 5 omega-3), and docosahexaenoic acid (DHA; 22: 6 omega-3) and plant-derived alpha-linolenic acid (ALA; 18: 3 omega-3) for incident CHD. DATA SOURCES A global consortium of 19 studies identified by November 2014. STUDY SELECTION Available prospective (cohort, nested case-control) or retrospective studies with circulating or tissue omega-3 biomarkers and ascertained CHD. DATA EXTRACTION AND SYNTHESIS Each study conducted standardized, individual-level analysis using harmonized models, exposures, outcomes, and covariates. Findings were centrally pooled using random-effects meta-analysis. Heterogeneity was examined by age, sex, race, diabetes, statins, aspirin, omega-6 levels, and FADS desaturase genes. MAIN OUTCOMES AND MEASURES Incident total CHD, fatal CHD, and nonfatal myocardial infarction (MI). RESULTS The 19 studies comprised 16 countries, 45 637 unique individuals, and 7973 total CHD, 2781 fatal CHD, and 7157 nonfatal MI events, with omega-3 measures in total plasma, phospholipids, cholesterol esters, and adipose tissue. Median age at baseline was 59 years (range, 18-97 years), and 28 660 (62.8%) were male. In continuous (per 1-SD increase) multivariable-adjusted analyses, the omega-3 biomarkers ALA, DPA, and DHA were associated with a lower risk of fatal CHD, with relative risks (RRs) of 0.91 (95% CI, 0.84-0.98) for ALA, 0.90 (95% CI, 0.85-0.96) for DPA, and 0.90 (95% CI, 0.84-0.96) for DHA. Although DPA was associated with a lower risk of total CHD (RR, 0.94; 95% CI, 0.90-0.99), ALA (RR, 1.00; 95% CI, 0.95-1.05), EPA (RR, 0.94; 95% CI, 0.87-1.02), and DHA (RR, 0.95; 95% CI, 0.91-1.00) were not. Significant associations with nonfatal MI were not evident. Associations appeared generally stronger in phospholipids and total plasma. Restricted cubic splines did not identify evidence of nonlinearity in dose responses. CONCLUSIONS AND RELEVANCE On the basis of available studies of free-living populations globally, biomarker concentrations of seafood and plant-derived omega-3 fatty acids are associated with a modestly lower incidence of fatal CHD. C1 [Del Gobbo, Liana C.; Yakoob, Mohammad Y.; Ingelsson, Erik] Stanford Univ, Dept Med, Sch Med, Div Cardiovasc Med, Stanford, CA 94305 USA. [Imamura, Fumiaki] Univ Cambridge, Sch Clin Med, Epidemiol Unit, Med Res Council,Inst Metab Sci, Cambridge, England. [Aslibekyan, Stella] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL USA. [Marklund, Matti; Riserus, Ulf] Uppsala Univ, Dept Publ Hlth & Caring Sci, Uppsala, Sweden. [Virtanen, Jyrki K.; Voutilainen, Sari] Univ Eastern Finland, Inst Publ Hlth & Clin Nutr, Joensuu, Finland. [Wennberg, Maria; Franks, Paul W.] Umea Univ, Dept Publ Hlth & Clin Med, Umea, Sweden. [Chiuve, Stephanie E.] Brigham & Womens Hosp, Dept Med, Div Prevent Med, 75 Francis St, Boston, MA 02115 USA. [Chiuve, Stephanie E.; Rimm, Eric B.; Stampfer, Meir; Willett, Walter C.] Harvard TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA USA. [dela Cruz, Luicito; Hodge, Allison M.] Canc Council Victoria, Canc Epidemiol Ctr, Melbourne, Vic, Australia. [Frazier-Wood, Alexis C.] ARS, USDA, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. [Fretts, Amanda M.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA. [Guallar, Eliseo; Woodward, Mark] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. [Matsumoto, Chisa] Tokyo Med Univ, Div Cardiol, Tokyo, Japan. [Matsumoto, Chisa; Djousse, Luc] Brigham & Womens Hosp, Div Aging, 75 Francis St, Boston, MA 02115 USA. [Matsumoto, Chisa; Djousse, Luc; Hu, Frank B.; Rimm, Eric B.; Stampfer, Meir; Willett, Walter C.] Harvard Med Sch, Boston, MA USA. [Prem, Kiesha; Koh, Woon-Puay; van Dam, Rob M.] Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore, Singapore. [Tanaka, Tosh] NIA, Translat Gerontol Branch, Bethesda, MD 20892 USA. [Wu, Jason H. Y.; Woodward, Mark] Univ Sydney, George Inst Global Hlth, Sydney Med Sch, Sydney, NSW, Australia. [Zhou, Xia; Steffen, Lyn M.; Steffen, Brian T.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Helmer, Catherine; Barberger-Gateau, Pascale; Samieri, Cecilia] Inst Sante Publ Epidemiol & Dev, Inst Natl Sante & Rech Med, Ctr Inst Natl Sante & Rech Med Epidemiol Biostat, Bordeaux, France. [Helmer, Catherine; Barberger-Gateau, Pascale; Samieri, Cecilia] Univ Bordeaux, Inst Sante Publ Epidemiol & Dev, Ctr Inst Natl Sante & Rech Med Epidemiol Biostat, Bordeaux, France. [Ingelsson, Erik; Lind, Lars] Uppsala Univ, Dept Med Sci, Uppsala, Sweden. [Yuan, Jian-Min] Univ Pittsburgh, Div Canc Control & Populat Sci, Inst Canc, Pittsburgh, PA USA. [Yuan, Jian-Min] Univ Pittsburgh, Dept Epidemiol, Grad Sch Publ Hlth, Pittsburgh, PA 15261 USA. [Campos, Hannia; Hu, Frank B.; Rimm, Eric B.; Stampfer, Meir; van Dam, Rob M.; Willett, Walter C.] Harvard TH Chan Sch Publ Hlth, Dept Nutr, Boston, MA USA. [Chaves, Paulo H. M.] Florida Int Univ, Benjamin Leon Ctr Geriatr Res & Educ, Miami, FL 33199 USA. [Gomez-Aracena, Jose] Univ Malaga, Dept Prevent Med, Malaga, Spain. [Hu, Frank B.; Rimm, Eric B.; Stampfer, Meir; Willett, Walter C.] Brigham & Womens Hosp, Dept Med, Channing Div Network Med, 75 Francis St, Boston, MA 02115 USA. [Johansson, Ingegerd] Umea Univ, Dept Odontol, Umea, Sweden. [Khaw, Kay-Tee; Luben, Robert N.] Univ Cambridge, Dept Publ Hlth & Primary Care, Cambridge, England. [Koh, Woon-Puay] Duke NUS Grad Med Sch Singapore, Singapore, Singapore. [Lemaitre, Rozenn N.] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA USA. [Franks, Paul W.] Lund Univ, Dept Clin Sci, Genet & Mol Epidemiol Unit, Lund, Sweden. [Siscovick, David S.] New York Acad Med, New York, NY USA. [Tsai, Michael Y.] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. [van Dam, Rob M.] Natl Univ Singapore, Dept Med, Yong Loo Lin Sch Med, Singapore, Singapore. [van Dam, Rob M.] Natl Univ Hlth Syst, Singapore, Singapore. [Woodward, Mark] Univ Oxford, George Inst Global Hlth, Nuffield Dept Publ Hlth, Oxford, England. [Mozaffarian, Dariush] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Del Gobbo, LC (reprint author), Stanford Univ, Dept Med, Sch Med, Div Cardiovasc Med, Stanford, CA 94305 USA. EM delgobbo@stanford.edu RI HELMER, Catherine/I-6581-2015; Samieri, Cecilia/E-3334-2014 OI HELMER, Catherine/0000-0002-5169-7421; Samieri, Cecilia/0000-0001-9809-7506 FU National Heart, Lung, and Blood Institute [HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, HHSN268201100012C, R01HL087641, R01HL59367, R01HL086694, HHSN268201200036C, HHSN268200800007C, N01HC55222, N01HC85079, N01HC85080]; National Human Genome Research Institute [U01HG004402]; National Institutes of Health [HHSN268200625226C, R01HL081549, UM1 CA167552, R01 HL35464, AA11181, HL35464, CA55075, HL60712, P30 DK46200, UM1 CA186107, R01 CA49449, R01 HL034594, P01CA87969, R01HL034594, R01HL088521, UM1 CA182876, R01CA 144034, UL1RR025005]; National Institute on Aging [R01AG023629]; Dutch Ministry of Health; Cancer Research Switzerland [AKT76]; Swiss National Science Foundation [32-9257-87]; Medical Research Council; Medical Research Council Epidemiology Unit [MC_UU_12015/5]; Intramural Research Program of the National Institutes of Health [N01-AG-916413, N01-AG821336, 263 MD 9164 13, 263 MD 821336]; Academy of Finland, Helsinki, Finland [1041086, 41471]; VicHealth; Australia's National Health and Medical Research Council [209057, 251553, 504711]; Swedish Cancer Society; Swedish Research Council; National Cancer Institute [R21 HL088081, CA-34944, CA-40360, CA-097193]; Fondation pour la Recherche Medicale; Caisse Nationale Maladie des Travailleurs Salaries; Direction Generale de la Sante; Institut de la Longevite; Conseils Regionaux d'Aquitaine et Bourgogne; Fondation de France; Ministry of Research-Institut National de la Sante et de la Recherche Medicale Programme Cohortes et collections de donnees biologiques; grant COGINUT from the Agence Nationale de la Recherche [ANR-06-PNRA-005]; grant FCS from the Fondation Plan Alzheimer; Caisse Nationale pour la Solidarite et l'Autonomie; Scottish Health Department Chief Scientist Organization; British Heart Foundation; FP Fleming Trust; Singapore National Medical Research Council [NMRC 1270/2010]; Swedish Research Council for Health, Working Life and Welfare; Uppsala City Council; National Institutes of Health Roadmap for Medical Research; National Institute of Neurological Disorders and Stroke; Commission of the European Communities; Cancer Research UK; Italian Ministry of Health [ICS 110.1\RS97.71]; Cancer Council Victoria; MGEN; Fondation Plan Alzheimer; [N01HC85082]; [N01HC85083]; [N01HC85086]; [U01HL080295]; [N01-HC-95159]; [N01-HC-95160]; [N01-HC-95161]; [N01-HC-95162]; [N01-HC-95163]; [N01-HC-95164]; [N01-HC-95165]; [N01-HC-95166]; [N01-HC-95167]; [N01-HC-95168]; [N01-HC-95169]; [UL1-TR-001079]; [UL1-TR000040]; [N02-HL-64278]; [HL-34595]; [HL-26490]; [N01HC85081] FX The Atherosclerosis Risk in Communities study was performed as a collaborative study supported by contracts HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, HHSN268201100012C, R01HL087641, R01HL59367, and R01HL086694 from the National Heart, Lung, and Blood Institute; contract U01HG004402 from the National Human Genome Research Institute; and contract HHSN268200625226C from the National Institutes of Health. We thank the staff and participants of the Atherosclerosis Risk in Communities study for their important contributions. Infrastructure was partly supported by grant UL1RR025005, a component of the National Institutes of Health and National Institutes of Health Roadmap for Medical Research. The Cardiovascular Health Study (CHS) was supported by contracts HHSN268201200036C, HHSN268200800007C, N01HC55222, N01HC85079, N01HC85080, N01HC85081, N01HC85082, N01HC85083, and N01HC85086 and grant U01HL080295 from the National Heart, Lung, and Blood Institute, with additional contribution from the National Institute of Neurological Disorders and Stroke. Additional support was provided by grant R01AG023629 from the National Institute on Aging. A full list of principal CHS investigators and institutions can be found at CHS-NHLBI. org. The Costa Rican adult study was supported by grant R01HL081549 from the National Institutes of Health. The European Study on Antioxidants, Myocardial Infarction and Cancer was supported by the Commission of the European Communities, as a Concerted Action within Directorate General-XII, with additional support from Directorate General-V Europe against Cancer. The national studies were financed by the Dutch Ministry of Health. Ulster Cancer Foundation and Milk Intervention Board, grant AKT76 from Cancer Research Switzerland, grant 32-9257-87 from the Swiss National Science Foundation, The Spanish Fondo de Investigaciones Sanitarias and Ministry of Science and Education, and German Federal Health Office. EPIC-Norfolk was funded by grants from Medical Research Council and Cancer Research UK. Dr Imamura received support from grant MC_UU_12015/5 from the Medical Research Council Epidemiology Unit. The Health Professionals Follow-up Study (HPFS) was supported by grants UM1 CA167552, R01 HL35464, AA11181, HL35464, CA55075, HL60712, and P30 DK46200 from the National Institutes of Health. The InChianti study was supported by grant ICS 110.1\RS97.71 as a targeted project by the Italian Ministry of Health and in part by contracts N01-AG-916413, N01-AG821336, 263 MD 9164 13, and 263 MD 821336 from the Intramural Research Program of the National Institutes of Health. The Kuopio Ischaemic Heart Disease Risk Factor Study was supported by grants 41471 and 1041086 from the Academy of Finland, Helsinki, Finland. The Melbourne Collaborative Cohort Study recruitment was funded by VicHealth and Cancer Council Victoria and was further supported by grants 209057, 251553, and 504711 from Australia's National Health and Medical Research Council and by infrastructure provided by Cancer Council Victoria. Cases and their vital status were ascertained through the Victorian Cancer Registry and the Australian Institute of Health and Welfare, including the National Death Index and the Australian Cancer Database. The Multi-Ethnic Study of Atherosclerosis (MESA) and the MESA SHARe project are conducted and supported by the National Heart, Lung, and Blood Institute in collaboration with MESA investigators.; Support for MESA is provided by contracts N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162, N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, N01-HC-95169, UL1-TR-001079, and UL1-TR000040 from the National Heart, Lung, and Blood Institute. Funding for SHARe genotyping was provided by contract N02-HL-64278 from the National Heart, Lung, and Blood Institute. Genotyping was performed at Affymetrix and the Broad Institute of Harvard and MIT using the Affymetrix Genome-Wide Human SNP Array 6.0. The Northern Sweden Health and Disease Studies I and II were supported by the Swedish Cancer Society and the Swedish Research Council. The Nurses Health Study was supported by research grants UM1 CA186107, R01 CA49449, R01 HL034594, P01CA87969, R01HL034594, and R01HL088521 from the National Institutes of Health. The Physician's Health Study was supported by grants R21 HL088081, CA-34944, CA-40360, and CA-097193 from the National Cancer Institute and grants HL-26490 and HL-34595 from the National Heart, Lung, and Blood Institute. The Three-City study was conducted under a partnership agreement between the Institut National de la Sante et de la Recherche Medicale, the University Bordeaux 2 Victor Segalen, and Sanofi. The Fondation pour la Recherche Medicale funded the preparation and initiation of the study. The Three-City study was also supported by the Caisse Nationale Maladie des Travailleurs Salaries, Direction Generale de la Sante, MGEN, Institut de la Longevite, Conseils Regionaux d'Aquitaine et Bourgogne, Fondation de France, Ministry of Research-Institut National de la Sante et de la Recherche Medicale Programme Cohortes et collections de donnees biologiques, grant COGINUT ANR-06-PNRA-005 from the Agence Nationale de la Recherche, grant FCS 2009-2012 from the Fondation Plan Alzheimer, and the Caisse Nationale pour la Solidarite et l'Autonomie. Dr Samieri was sponsored by a grant from the Fondation Plan Alzheimer. The Scottish Heart Health Extended Cohort study was funded by the Scottish Health Department Chief Scientist Organization, British Heart Foundation, and FP Fleming Trust. The Singapore Chinese Health Study was supported by grant NMRC 1270/2010 from the Singapore National Medical Research Council and grants R01CA 144034 and UM1 CA182876 from the National Institutes of Health. The Uppsala Longitudinal Studies of Adult Men 50 and 70 were funded by the Swedish Research Council for Health, Working Life and Welfare, Uppsala City Council, and Swedish Research Council. NR 58 TC 9 Z9 9 U1 15 U2 16 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6106 EI 2168-6114 J9 JAMA INTERN MED JI JAMA Intern. Med. PD AUG PY 2016 VL 176 IS 8 BP 1155 EP 1166 DI 10.1001/jamainternmed.2016.2925 PG 12 WC Medicine, General & Internal SC General & Internal Medicine GA DT0AN UT WOS:000381145000027 PM 27357102 ER PT J AU Miks-Krajnik, M Yoon, YJ Ukuku, DO Yuk, HG AF Miks-Krajnik, Marta Yoon, Yong-Jin Ukuku, Dike O. Yuk, Hyun-Gyun TI Identification and Quantification of Volatile Chemical Spoilage Indexes Associated with Bacterial Growth Dynamics in Aerobically Stored Chicken SO JOURNAL OF FOOD SCIENCE LA English DT Article DE acetic acid; ethanol; 3-methyl-1-butanol; multivariate analysis; shelf life ID MEAT SPOILAGE; TEMPERATURE CONDITIONS; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; ORGANIC-COMPOUNDS; PRODUCTS; QUALITY; STORAGE; BEEF AB Volatile organic compounds (VOCs) as chemical spoilage indexes (CSIs) of raw chicken breast stored aerobically at 4, 10, and 21 degrees C were identified and quantified using solid phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS). The growth dynamics of total viable count (TVC), psychrotrophs, Pseudomonas spp., lactic acid bacteria (LAB), Brochothrix thermosphacta and H2S producing bacteria were characterized based on maximum growth rates (mu(max)), maximal microbial concentration (N-max) and at the moment of microbial shelf life (S-values), calculated from Gompertz-fitted growth curves. Pseudomonas spp. was predominant species, while B. thermosphacta was characterized by the highest mu(max). The microbiological and sensory shelf lives were estimated based on TVC, Pseudomonas spp., and B. thermosphacta counts and sensory evaluation, respectively. Among 27 VOCs identified by GC-MS in spoiled chicken samples, ethanol (EtOH), 1-butanol-3-methyl (1But-3M), and acetic acid (C-2) achieved the highest Pearson's correlation coefficients of 0.66, 0.61, and 0.59, respectively, with TVC, regardless of storage temperature. Partial least squares (PLS) regression revealed that the synthesis of 1But-3M and C-2 was most likely induced by the metabolic activity of B. thermosphacta and LAB, while EtOH was attributed to Pseudomonas spp. The increase in concentration of selected volatile spoilage markers (EtOH, 1But-3M, and C2) in the headspace over spoiled chicken breast was found to be statistically significant (P < 0.05) with TVC growth. These findings highlight the possibility of analyzing the combination of 3 selected spoilage markers: EtOH, 1But-3M, and C-2 as rapid evaluation for poultry quality testing using SPME-GC-MS. C1 [Miks-Krajnik, Marta; Yuk, Hyun-Gyun] Natl Univ Singapore, Dept Chem, Food Sci & Technol Programme, 3 Sci Dr 3, Singapore 117543, Singapore. [Yuk, Hyun-Gyun] Natl Univ Singapore Suzhou Res Inst, 377 Linquan St,Suzhou Ind Pk, Suzhou 215123, Jiangsu, Peoples R China. [Miks-Krajnik, Marta] Univ Warmia & Mazury, Chair Ind & Food Microbiol, Fac Food Sci, Plac Cieszynski 1, PL-10726 Olsztyn, Poland. [Yoon, Yong-Jin] Nanyang Technol Univ, Sch Mech & Aerosp Engn, Div Engn Mech, 50 Nanyang Ave, Singapore 639798, Singapore. [Ukuku, Dike O.] ARS, Food Safety Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, 600 East,Mermaid Lane, Wyndmoor, PA 19038 USA. RP Yuk, HG (reprint author), Natl Univ Singapore, Dept Chem, Food Sci & Technol Programme, 3 Sci Dr 3, Singapore 117543, Singapore.; Yuk, HG (reprint author), Natl Univ Singapore Suzhou Res Inst, 377 Linquan St,Suzhou Ind Pk, Suzhou 215123, Jiangsu, Peoples R China. EM chmyukhg@nus.edu.sg RI Yoon, Yong-Jin/A-3860-2011 OI Yoon, Yong-Jin/0000-0002-3885-4947 FU A*STAR/Science & Engineering Research Council - SERC research project [112 177 0039/R-143-000-511-305] FX This study was financially supported by A*STAR/Science & Engineering Research Council - SERC research project (grant no. 112 177 0039/R-143-000-511-305). Authors would like to acknowledge Prof. Ireneusz Bialobrzewski from Univ. of Warmia and Mazury in Olsztyn (Poland). NR 39 TC 0 Z9 0 U1 20 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD AUG PY 2016 VL 81 IS 8 BP M2006 EP M2014 DI 10.1111/1750-3841.13371 PG 9 WC Food Science & Technology SC Food Science & Technology GA DV0JX UT WOS:000382604900022 PM 27332555 ER PT J AU Muturi, EJ Bara, JJ Rooney, AP Hansen, AK AF Muturi, Ephanius J. Bara, Jeffrey J. Rooney, Alejandro P. Hansen, Allison K. TI Midgut fungal and bacterial microbiota of Aedes triseriatus and Aedes japonicus shift in response to La Crosse virus infection SO MOLECULAR ECOLOGY LA English DT Article DE bacteria; fungi; La Crosse virus; mosquito ID DIPTERA-CULICIDAE L.; ANOPHELES-STEPHENSI; GUT MICROBIOTA; UNITED-STATES; VECTOR COMPETENCE; MOSQUITO VECTOR; DIVERSITY; AEGYPTI; INSECTS; BEETLE AB Understanding how midgut microbial communities of field-collected mosquitoes interact with pathogens is critical for controlling vector infection and disease. We used 16S rRNA and internal transcribed spacer sequencing to characterize the midgut bacterial and fungal communities of adult females of Aedes triseriatus and Aeries japonicus collected as pupae in tree holes, plastic bins and waste tires and their response to La Crosse virus (LACV) infection. For both mosquito species and across all habitat and virus treatments, a total of 62 bacterial operational taxonomic units (OTUs) from six phyla and 21 fungal OTUs from two phyla were identified. The majority of bacterial (9251) and fungal (71%) OTUs were shared between the mosquito species; however, several OTUs were unique to each species. Bacterial and fungal communities of individuals that took either infectious or noninfectious bloodmeals were less diverse and more homogeneous compared to those of newly emerged adults. Interestingly, LACVinfected A. triseriatus and A. japonicus had higher bacterial richness and lower fungal richness compared to individuals that took a noninfectious bloodmeal, suggesting that viral infection was associated with an increase in bacterial OTUs and a decrease in fungal OTUs. For both mosquito species, several OTUs were identified that had both high fidelity and specificity to mosquito midguts that were infected with LACV. Overall, these findings demonstrate that bacterial and fungal communities that reside in mosquito midguts respond to host diet and viral infection and could play a role in modulating vector susceptibility to LACV. C1 [Muturi, Ephanius J.; Bara, Jeffrey J.] Univ Illinois, Illinois Nat Hist Survey, 1816 S Oak St, Champaign, IL 61820 USA. [Bara, Jeffrey J.] Univ Louisville, Dept Biol, Louisville, KY 40292 USA. [Rooney, Alejandro P.] ARS, Crop Bioprotect Res Unit, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Hansen, Allison K.] Univ Illinois, Dept Entomol, 505 S Goodwin Ave, Urbana, IL 61801 USA. RP Muturi, EJ (reprint author), ARS, Crop Bioprotect Res Unit, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM ephajumu@yahoo.com FU Used Tire Fund and Emergency Public Health Fund from the State of Illinois; University of Illinois, Urbana-Champaign FX We thank Andrew Donelson, Khamisha Wilson and Michael Kozak for their assistance in mosquito collection and identification and Chang-Hyun Kim for his help with DNA extraction and data validation by real-time qPCR. We are also grateful to Dr. Robert Tesh and the World Reference Center for Emerging Viruses and Arboviruses at University of Texas for the generous donation of LACV strain TX-AR 9-6319 that was used in this study. This study was supported by the Used Tire Fund and Emergency Public Health Fund from the State of Illinois to E.J.M and start-up funds from the University of Illinois, Urbana-Champaign to A.K.H for data analysis. NR 78 TC 2 Z9 2 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD AUG PY 2016 VL 25 IS 16 BP 4075 EP 4090 DI 10.1111/mec.13741 PG 16 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA DT6FD UT WOS:000381578200021 PM 27357374 ER PT J AU Isaza, REA Diaz-Trujillo, C Dhillon, B Aerts, A Carlier, J Crane, CF de Jong, TV de Vries, I Dietrich, R Farmer, AD Fereira, CF Garcia, S Guzman, M Hamelin, RC Lindquist, EA Mehrabi, R Quiros, O Schmutz, J Shapiro, H Reynolds, E Scalliet, G Souza, M Stergiopoulos, I Van der Lee, TAJ De Wit, PJGM Zapater, MF Zwiers, LH Grigoriev, IV Goodwin, SB Kema, GHJ AF Arango Isaza, Rafael E. Diaz-Trujillo, Caucasella Dhillon, Braham Aerts, Andrea Carlier, Jean Crane, Charles F. de Jong, Tristan V. de Vries, Ineke Dietrich, Robert Farmer, Andrew D. Fereira, Claudia Fortes Garcia, Suzana Guzman, Mauricio Hamelin, Richard C. Lindquist, Erika A. Mehrabi, Rahim Quiros, Olman Schmutz, Jeremy Shapiro, Harris Reynolds, Elizabeth Scalliet, Gabriel Souza, Manoel, Jr. Stergiopoulos, Ioannis Van der Lee, Theo A. J. De Wit, Pierre J. G. M. Zapater, Marie-Francoise Zwiers, Lute-Harm Grigoriev, Igor V. Goodwin, Stephen B. Kema, Gert H. J. TI Combating a Global Threat to a Clonal Crop: Banana Black Sigatoka Pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) Genomes Reveal Clues for Disease Control SO PLOS GENETICS LA English DT Article ID LEAF STREAK DISEASE; INDUCED POINT MUTATIONS; DE-NOVO IDENTIFICATION; CAUSAL AGENT; ELECTROPHORETIC KARYOTYPE; TRANSPOSABLE ELEMENTS; LEPTOSPHAERIA-MACULANS; LTR RETROTRANSPOSONS; EUKARYOTIC GENOMES; GENETIC-STRUCTURE AB Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is the most significant foliar disease of banana worldwide. Due to the lack of effective host resistance, management of this disease requires frequent fungicide applications, which greatly increase the economic and environmental costs to produce banana. Weekly applications in most banana plantations lead to rapid evolution of fungicide-resistant strains within populations causing disease-control failures throughout the world. Given its extremely high economic importance, two strains of P. fijiensis were sequenced and assembled with the aid of a new genetic linkage map. The 74-Mb genome of P. fijiensis is massively expanded by LTR retrotransposons, making it the largest genome within the Dothideomycetes. Melting-curve assays suggest that the genomes of two closely related members of the Sigatoka disease complex, P. eumusae and P. musae, also are expanded. Electrophoretic karyotyping and analyses of molecular markers in P. fijiensis field populations showed chromosome-length polymorphisms and high genetic diversity. Genetic differentiation was also detected using neutral markers, suggesting strong selection with limited gene flow at the studied geographic scale. Frequencies of fungicide resistance in fungicide-treated plantations were much higher than those in untreated wild-type P. fijiensis populations. A homologue of the Cladosporium fulvum Avr4 effector, PfAvr4, was identified in the P. fijiensis genome. Infiltration of the purified PfAVR4 protein into leaves of the resistant banana variety Calcutta 4 resulted in a hypersensitive-like response. This result suggests that Calcutta 4 could carry an unknown resistance gene recognizing PfAVR4. Besides adding to our understanding of the overall Dothideomycete genome structures, the P. fijiensis genome will aid in developing fungicide treatment schedules to combat this pathogen and in improving the efficiency of banana breeding programs. C1 [Arango Isaza, Rafael E.] Univ Nacl Colombia, Escuela Biociencias, Medellin, Colombia. [Arango Isaza, Rafael E.] Univ Nacl Colombia, Plant Biotechnol Unit, Corp Invest Biol, Medellin, Colombia. [Diaz-Trujillo, Caucasella; de Jong, Tristan V.; de Vries, Ineke; Van der Lee, Theo A. J.; Kema, Gert H. J.] Wageningen Univ & Res, Plant Res Int, Wageningen, Netherlands. [Dhillon, Braham] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Aerts, Andrea; Lindquist, Erika A.; Schmutz, Jeremy; Shapiro, Harris; Grigoriev, Igor V.] US Dept Energy Joint Genome Inst, Walnut Creek, CA USA. [Carlier, Jean; Zapater, Marie-Francoise] CIRAD, UMR BGPI, Montpellier, France. [Crane, Charles F.; Goodwin, Stephen B.] USDA ARS, W Lafayette, IN USA. [Dietrich, Robert] Syngenta Biotechnol Inc, Res Triangle Pk, NC USA. [Farmer, Andrew D.] Natl Ctr Genome Resources, Santa Fe, NM USA. [Fereira, Claudia Fortes] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. [Garcia, Suzana] Univ Lavras, Lavras, Brazil. [Guzman, Mauricio; Quiros, Olman] Natl Banana Corp Costa Rica CORBANA, La Rita De Pococi, Limon, Costa Rica. Univ British Columbia, Dept Forest & Conservat Sci, Vancouver, BC, Canada. [Hamelin, Richard C.] Laurentian Forestry Ctr, Nat Resources Canada, Canadian Forest Serv, Quebec City, PQ, Canada. [Mehrabi, Rahim] Seed & Plant Improvement Inst, Dept Genet, Karaj, Iran. [Reynolds, Elizabeth] Syngenta Crop protect Jeallots Hill Int Res Ctr, Gen Bioinformat, Bracknell, Berks, England. [Scalliet, Gabriel] Munchwilen AG, Syngenta Crop Protect, Stein, Switzerland. [Souza, Manoel, Jr.] Embrapa LABEX Europe, Wageningen, Netherlands. [Stergiopoulos, Ioannis] Univ Calif Davis, Davis, CA 95616 USA. [De Wit, Pierre J. G. M.; Kema, Gert H. J.] Wageningen Univ, Lab Phytopathol, Wageningen, Netherlands. [Zwiers, Lute-Harm] CBS KNAW Fungal Div Ctr, Utrecht, Netherlands. [Dhillon, Braham] Univ Arkansas, Fayetteville, AR 72701 USA. [de Jong, Tristan V.] Univ Groningen, Univ Med Ctr Groningen, Groningen, Netherlands. [Garcia, Suzana] KeyGene, Wageningen, Netherlands. [Mehrabi, Rahim] Seed & Plant Improvement Inst, Karaj, Iran. [Quiros, Olman] Lab LAMA SA, San Jose, Costa Rica. [Shapiro, Harris] WaferGen Inc, Fremont, CA USA. [Souza, Manoel, Jr.] Embrapa Agroenergy, Brasilia, DF, Brazil. [Zwiers, Lute-Harm] Lectoraat Biobased Prod, Avans Hsch, Breda, Netherlands. RP Kema, GHJ (reprint author), Wageningen Univ & Res, Plant Res Int, Wageningen, Netherlands.; Goodwin, SB (reprint author), USDA ARS, W Lafayette, IN USA.; Kema, GHJ (reprint author), Wageningen Univ, Lab Phytopathol, Wageningen, Netherlands. EM Steve.Goodwin@ARS.USDA.gov; gert.kema@wur.nl RI Mehrabi, Rahim/S-1800-2016; OI Mehrabi, Rahim/0000-0002-5098-9123; de Jong, Tristan/0000-0003-0385-0089 FU Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]; USDA CRIS [3602-22000-015-00D, 3602-22000-017-00D]; Dutch Dioraphte VSM grant [14.03.01.00]; Colombian Administrative department of Science, Technology and Innovation - Colciencias [0031-2013, 018-2015]; National University of Colombia at Medellin; National Council of Science and Technology (CONACyT) from Mexico [187781] FX The work conducted by the U.S. Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. This work was supported in part by USDA CRIS projects 3602-22000-015-00D and 3602-22000-017-00D. Work at Plant Research International was partly funded by The Dutch Dioraphte VSM grant 14.03.01.00. UNALMED and CIB work was supported by the Colombian Administrative department of Science, Technology and Innovation - Colciencias contracts no 0031-2013 and No 018-2015, and by the National University of Colombia at Medellin. CDT's research work was supported by scholarship 187781 from the National Council of Science and Technology (CONACyT) from Mexico. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 126 TC 0 Z9 0 U1 18 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD AUG PY 2016 VL 12 IS 8 AR e1005876 DI 10.1371/journal.pgen.1005876 PG 36 WC Genetics & Heredity SC Genetics & Heredity GA DU7LA UT WOS:000382394500001 ER PT J AU Friesen, TL AF Friesen, Timothy L. TI Combating the Sigatoka Disease Complex on Banana SO PLOS GENETICS LA English DT Editorial Material ID PATHOGEN C1 [Friesen, Timothy L.] ARS, Cereal Crops Res Unit, USDA, Fargo, ND 58105 USA. RP Friesen, TL (reprint author), ARS, Cereal Crops Res Unit, USDA, Fargo, ND 58105 USA. EM timothy.friesen@ars.usda.gov NR 12 TC 1 Z9 1 U1 3 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD AUG PY 2016 VL 12 IS 8 AR e1006234 DI 10.1371/journal.pgen.1006234 PG 4 WC Genetics & Heredity SC Genetics & Heredity GA DU7LA UT WOS:000382394500031 PM 27513644 ER PT J AU Kiedrowicz, A Rector, BG Zawierucha, K Szydlo, W Skoracka, A AF Kiedrowicz, Agnieszka Rector, Brian G. Zawierucha, Krzysztof Szydlo, Wiktoria Skoracka, Anna TI Phytophagous mites (Acari: Eriophyoidea) recorded from Svalbard, including the description of a new species SO POLAR BIOLOGY LA English DT Article DE Arctic biology; Eriophyidae; Extreme environments; Molecular taxonomy; Herbivorous mites; DNA barcoding ID WHEAT CURL MITE; DNA BARCODE DATA; FRAM STRAIT; SPITSBERGEN; HOST; SPECIALIZATION; BIODIVERSITY; SEA; TERRESTRIAL; ERIOPHYIDAE AB Eriophyoid mites (Eriophyoidea) are minute phytophagous mites with great economic importance and great invasive potential. In spite of their demonstrated impact on ecosystem functions, knowledge of eriophyoid mite fauna in the Arctic is lacking. To date, only eight eriophyoid mite species have been recorded from the entire region north of the Arctic Circle. The Svalbard archipelago is one of the most biologically investigated Arctic areas. Despite the fact that studies on invertebrates on Svalbard have been conducted for more than one hundred years, eriophyoids have never been recorded before from this place, except for one likely accidental record of a single specimen belonging to the genus Eriophyes. Thus, each new study of eriophyoid mite fauna in this region is important. In this paper, a new species of eriophyoid mite, Cecidophyes siedleckii n. sp., is described and illustrated. Nucleotide sequence data (D2 region of 28S rDNA) were employed to complement traditional morphological taxonomy. The first record of Aceria saxifragae (Rostrup 1900) from Svalbard is also provided, with supplementary morphological descriptions and illustrations. Eriophyoid mites represent an important and underutilized taxon that is available to ecologists studying the effects of changing climatic conditions on Svalbard. C1 [Kiedrowicz, Agnieszka; Zawierucha, Krzysztof; Szydlo, Wiktoria; Skoracka, Anna] Adam Mickiewicz Univ, Inst Environm Biol, Dept Anim Taxon & Ecol, Umultowska 89, PL-60687 Poznan, Poland. [Rector, Brian G.] USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. RP Kiedrowicz, A (reprint author), Adam Mickiewicz Univ, Inst Environm Biol, Dept Anim Taxon & Ecol, Umultowska 89, PL-60687 Poznan, Poland. EM kiedra@amu.edu.pl RI Kiedrowicz, Agnieszka/K-5181-2016; OI Szydlo, Wiktoria/0000-0001-5990-5611; Kiedrowicz, Agnieszka/0000-0002-2425-7885; Skoracka, Anna/0000-0002-9485-532X FU Faculty of Biology, Adam Mickiewicz University (AMU), Poznan, Poland; Polish Ministry of Science and Higher Education via the "Diamond Grant" programme [DIA 2011035241] FX The study was financially supported by Faculty of Biology, Adam Mickiewicz University (AMU), Poznan, Poland, and partially by the Polish Ministry of Science and Higher Education via the "Diamond Grant" programme (Grant No. DIA 2011035241 to K.Z.). The authors are grateful to Prof. James Jr. Amrine for helpful information regarding eriophyoid mites occurring in Arctic, Dr. Nikolaj Scharff for information about type material of Aceria saxifragae, Dr. Ziemowit Olszanowski (AMU) for the assistance regarding taxonomical nomenclature, and Prof. Karol Latowski (AMU) for the confirmation of the botanical taxonomic identification. NR 67 TC 1 Z9 1 U1 4 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0722-4060 EI 1432-2056 J9 POLAR BIOL JI Polar Biol. PD AUG PY 2016 VL 39 IS 8 BP 1359 EP 1368 DI 10.1007/s00300-015-1858-x PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DT3UA UT WOS:000381405400001 ER PT J AU Quemada, M Daughtry, CST AF Quemada, Miguel Daughtry, Craig S. T. TI Spectral Indices to Improve Crop Residue Cover Estimation under Varying Moisture Conditions SO REMOTE SENSING LA English DT Article DE cellulose absorption index; shortwave infrared normalized difference residue index; normalized difference tillage index; spectral moisture index; water content indices ID WATER-CONTENT; TILLAGE PRACTICES; SOIL REFLECTANCE; DECOMPOSITION; VEGETATION; HYPERION; LANDSAT; IMAGERY; MODEL; ASTER AB Crop residues on the soil surface protect the soil against erosion, increase water infiltration and reduce agrochemicals in runoff water. Crop residues and soils are spectrally different in the absorption features associated with cellulose and lignin. Our objectives were to: (1) assess the impact of water on the spectral indices for estimating crop residue cover (f(R)); (2) evaluate spectral water indices for estimating the relative water content (RWC) of crop residues and soils; and (3) propose methods that mitigate the uncertainty caused by variable moisture conditions on estimates of f(R). Reflectance spectra of diverse crops and soils were acquired in the laboratory over the 400-2400-nm wavelength region. Using the laboratory data, a linear mixture model simulated the reflectance of scenes with various f(R) and levels of RWC. Additional reflectance spectra were acquired over agricultural fields with a wide range of crop residue covers and scene moisture conditions. Spectral indices for estimating crop residue cover that were evaluated in this study included the Normalized Difference Tillage Index (NDTI), the Shortwave Infrared Normalized Difference Residue Index (SINDRI) and the Cellulose Absorption Index (CAI). Multivariate linear models that used pairs of spectral indices-one for RWC and one for f(R)-significantly improved estimates of f(R) using CAI and SINDRI. For NDTI to reliably assess f(R), scene RWC should be relatively dry (RWC < 0.25). These techniques provide the tools needed to monitor the spatial and temporal changes in crop residue cover and help determine where additional conservation practices may be required. C1 [Quemada, Miguel] Tech Univ Madrid, Sch Agr Engn, Madrid 28040, Spain. [Quemada, Miguel] Tech Univ Madrid, CEIGRAM, Madrid 28040, Spain. [Daughtry, Craig S. T.] USDA ARS, Hydrol & Remote Sensing Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. RP Quemada, M (reprint author), Tech Univ Madrid, Sch Agr Engn, Madrid 28040, Spain.; Quemada, M (reprint author), Tech Univ Madrid, CEIGRAM, Madrid 28040, Spain. EM miguel.quemada@upm.es; Craig.Daughtry@ARS.USDA.GOV RI Quemada, Miguel/K-4214-2014 OI Quemada, Miguel/0000-0001-5793-2835 FU Spanish Ministry of Education through program Salvador de Madariaga; Spanish Ministry of Economy and Competitiveness [AGL201452310R]; Comunidad de Madrid [S2013/ABI2717]; USDA-Agricultural Research Service FX This research was funded by the Spanish Ministry of Education through the program Salvador de Madariaga and the Spanish Ministry of Economy and Competitiveness (AGL201452310R), the Comunidad de Madrid (S2013/ABI2717) and USDA-Agricultural Research Service. We would like to thank the staff from USDA-ARS and the U.S. Forest Service International Visitor Program for their helpful assistance. USDA is an equal opportunity provider and employer. NR 50 TC 1 Z9 1 U1 10 U2 10 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD AUG PY 2016 VL 8 IS 8 AR 660 DI 10.3390/rs8080660 PG 20 WC Remote Sensing SC Remote Sensing GA DU8JI UT WOS:000382458700047 ER PT J AU Parasar, P Wilhelm, A Rutigliano, HM Thomas, AJ Teng, LH Shi, B Davis, WC Suarez, CE New, DD White, KL Davies, CJ AF Parasar, Parveen Wilhelm, Amanda Rutigliano, Heloisa M. Thomas, Aaron J. Teng, Lihong Shi, Bi Davis, William C. Suarez, Carlos E. New, Daniel D. White, Kenneth L. Davies, Christopher J. TI Expression of bovine non-classical major histocompatibility complex class I proteins in mouse P815 and human K562 cells SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Bovine leukocyte antigens (BMA); Human K562 cells; Major histocompatibility complex; Mouse P815 cells; Non-classical MHC class I ID MHC CLASS-I; PARASITE THEILERIA-PARVA; ANTIGEN BOLA WORKSHOP; NATURAL-KILLER-CELLS; CATTLE KIR GENES; SOLUBLE HLA-G; SURFACE EXPRESSION; NOMENCLATURE COMMITTEE; GENOMIC LOCATION; JOINT REPORT AB Major histocompatibility complex class I (MHC-1) proteins can be expressed as cell surface or secreted proteins. To investigate whether bovine non-classical MHC-I proteins are expressed as cell surface or secreted proteins, and to assess the reactivity pattern of monoclonal antibodies with non-classical MHC-1 isoforms, we expressed the MHC proteins in murine P815 and human 1(562 (MHC-1 deficient) cells. Following antibiotic selection, stably transfected cell lines were stained with H1A or W6/32 antibodies to detect expression of the MHC-I proteins by flow cytometry. Two non-classical proteins (BoLA-NC1*00501 and BoLA-NC3*00101) were expressed on the cell surface in both cell lines. Surprisingly, the BoLA-NC4*00201 protein was expressed on the cell membrane of human 1(562 but not mouse P815 cells. Two non-classical proteins (BoLA-NC1*00401, which lacks a transmembrane domain, and BoLA-NC2*00102) did not exhibit cell surface expression. Nevertheless, Western blot analyses demonstrated expression of the MHC-I heavy chain in all transfected cell lines. Ammonium-sulfate precipitation of proteins from culture supernatants showed that BoLA-NC1*00401 was secreted and that all surface expressed proteins where shed from the cell membrane by the transfected cells. Interestingly, the surface expressed MHC-1 proteins were present in culture supernatants at a much higher concentration than BoLA-NC1*00401. This comprehensive study shows that bovine non-classical MHC-I proteins BoLA-NC1*00501, BoLA-NC3*00101, and BoLA-NC4*00201 are expressed as surface isoforms with the latter reaching the cell membrane only in 1(562 cells. Furthermore, it demonstrated that BoLA-NC1*00401 is a secreted isoform and that significant quantities of membrane associated MHC-I proteins can be shed from the cell membrane. (C) 2016 The Authors. Published by Elsevier Ltd. C1 [Parasar, Parveen; Wilhelm, Amanda; Rutigliano, Heloisa M.; Thomas, Aaron J.; Teng, Lihong; Shi, Bi; White, Kenneth L.; Davies, Christopher J.] Utah State Univ, Dept Anim Dairy & Vet Sci, 4815 Old Main Hill, Logan, UT 84322 USA. [Rutigliano, Heloisa M.; White, Kenneth L.; Davies, Christopher J.] Utah State Univ, Sch Vet Med, 4815 Old Main Hill, Logan, UT 84322 USA. [Parasar, Parveen; Wilhelm, Amanda; Thomas, Aaron J.; Teng, Lihong; Shi, Bi; White, Kenneth L.; Davies, Christopher J.] Utah State Univ, Ctr Integrated BioSyst, 4815 Old Main Hill, Logan, UT 84322 USA. [Davis, William C.; New, Daniel D.] Washington State Univ, Dept Vet Microbiol & Pathol, POB 647040, Pullman, WA 99164 USA. [Suarez, Carlos E.] Washington State Univ, USDA, ARS, Anim Dis Res Unit, POB 646630, Pullman, WA 99164 USA. RP Davies, CJ (reprint author), Utah State Univ, Sch Vet Med, 4815 Old Main Hill, Logan, UT 84322 USA. EM chris.davies@usu.edu OI Parasar, Parveen/0000-0002-5967-3758 FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67015-30008] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-30008 from the USDA National Institute of Food and Agriculture. The funding agency had no role in study design, data collection and analysis, or preparation of this article. NR 65 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD AUG PY 2016 VL 107 BP 161 EP 170 DI 10.1016/j.rvsc.2016.06.004 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA DT5RZ UT WOS:000381541600024 PM 27473990 ER PT J AU Fiuza, VRD Lopes, CWG Cosendey, RIJ de Oliveira, FCR Fayer, R Santin, M AF da Silva Fiuza, Vagner Ricardo Gomes Lopes, Carlos Wilson Juliboni Cosendey, Rachel Ingrid Rodrigues de Oliveira, Francisco Carlos Fayer, Ronald Santin, Monica TI Zoonotic Enterocytozoon bieneusi genotypes found in brazilian sheep SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE E. bieneusi; Genotyping; Microsporidia; PCR; Public health; Zoonosis ID EASTERN UNITED-STATES; VIRUS-INFECTED PATIENTS; DAIRY-CATTLE; MOLECULAR CHARACTERIZATION; ENCEPHALITOZOON-CUNICULI; 1ST REPORT; GIARDIA-DUODENALIS; GENETIC DIVERSITY; NORTHEAST CHINA; HIGH PREVALENCE AB The presence of Enterocytozoon bieneusi in sheep has been reported in only three countries worldwide. The present study has found E. bieneusi in Brazilian sheep for the first time; in 24/125 (19.2%) fecal samples by PCR and on 8/10 (80%) farms from three diverse locations. A significantly greater number of lambs (34.1%) were found infected than older sheep (11.1%) (P = 0.0036); most of the lambs were less than 6 months of age. Farms with an intensive production system had a lower infection rate (10.5%) of infection than semi-intensive farms (23%), but this difference was not statistically significant Sequencing analysis of the internal transcribed spacer (ITS) region of the rRNA gene revealed four known E. bieneusi genotypes (BEB6, BEB7, I, and LW1) and two novel genotypes (BEB18 and BEB19). Genotypes LW1 and BEB19 clustered within designated zoonotic Group 1 while genotypes BEB6, BEB7,1, and BEB18, and clustered within Group 2. BEB6 was the most prevalent (45.8%), followed by BEB7 (33.3%). Genotypes BEB6, I, and LW1 are zoonotic and can pose a risk to human health for immunocompromised individuals. Published by Elsevier Ltd. C1 [da Silva Fiuza, Vagner Ricardo; Fayer, Ronald; Santin, Monica] ARS, Environm Microbial & Food Safety Lab, USDA, 10300 Baltimore Ave,BARC East,Bldg 173, Beltsville, MD 20705 USA. [da Silva Fiuza, Vagner Ricardo; Gomes Lopes, Carlos Wilson] UFRRJ, Inst Vet, Dept Parasitol Anim, BR 465 Km 7, BR-23897970 Seropedica, RJ, Brazil. [Juliboni Cosendey, Rachel Ingrid] Univ Fed Fluminense, HUAP, Lab Multiusuario Apoio Pesquisa Nefrol & Ciencias, BR-24033900 Niteroi, RJ, Brazil. [Rodrigues de Oliveira, Francisco Carlos] Univ Estadual N Fluminen, Lab Sanidade Anim, BR-28013602 Campos dos Goytacazes, RJ, Brazil. RP Santin, M (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, 10300 Baltimore Ave,BARC East,Bldg 173, Beltsville, MD 20705 USA. EM monica.santin-duran@ars.usda.gov NR 99 TC 0 Z9 0 U1 3 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD AUG PY 2016 VL 107 BP 196 EP 201 DI 10.1016/j.rvsc.2016.06.006 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA DT5RZ UT WOS:000381541600029 PM 27473995 ER PT J AU Badau, F Fare, R Gopinath, M AF Badau, Flavius Fare, Rolf Gopinath, Munisamy TI Global resilience to climate change: Examining global economic and environmental performance resulting from a global carbon dioxide market SO RESOURCE AND ENERGY ECONOMICS LA English DT Article DE Cap and trade; Re-allocation models; Directional distance function; Shadow prices; Carbon dioxide (CO2); Polluting technology ID MARGINAL ABATEMENT COSTS; TECHNICAL EFFICIENCY; DISTANCE FUNCTIONS; SHADOW PRICE; EMISSIONS; ENERGY; TECHNOLOGY; POLLUTION; BENEFIT; MODEL AB In this study, we investigated global economic and environmental resilience in the presence of climate change. In particular, we examine the possibility of mitigating carbon dioxide (CO2) emissions without stressing standards of living. Here, we set up a cross-country CO2 market constrained by a quota, where CO2 is optimally re-allocated based on relative shadow prices of the pollutant. The objective is to stabilize global emissions without hindering global incomes and in the process achieve a single CO2 price. We introduce a re-allocation model that takes into account each country's underlying polluting technology. The model solutions are then used to investigate whether a single, global price for CO2 is attainable. Our results suggest that global CO2 emissions could stabilize without stressing global incomes, with a global CO2 market achieving equilibrium. With a CO2 market, countries would then have the incentive to consider adopting, improving, or investing in additional abatement technologies to move beyond current capabilities, while continuing to increase standards of living. Published by Elsevier B.V. C1 [Badau, Flavius; Gopinath, Munisamy] USDA, Market & Trade Econ Div, ERS, Washington, DC USA. [Fare, Rolf] Oregon State Univ, 319 Ballard Extens Hall, Corvallis, OR 97331 USA. RP Badau, F (reprint author), USDA, Market & Trade Econ Div, ERS, Washington, DC USA. EM flavius.badau@ers.usda.gov; rolf.fare@oregonstate.edu; gmunisamy@ers.usda.gov NR 30 TC 0 Z9 0 U1 11 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0928-7655 EI 1873-0221 J9 RESOUR ENERGY ECON JI Resour. Energy Econ. PD AUG PY 2016 VL 45 BP 46 EP 64 DI 10.1016/j.reseneeco.2016.05.002 PG 19 WC Economics; Energy & Fuels; Environmental Sciences; Environmental Studies SC Business & Economics; Energy & Fuels; Environmental Sciences & Ecology GA DV0FM UT WOS:000382593400004 ER PT J AU McMillen, H Campbell, LK Svendsen, ES Reynolds, R AF McMillen, Heather Campbell, Lindsay K. Svendsen, Erika S. Reynolds, Renae TI Recognizing Stewardship Practices as Indicators of Social Resilience: In Living Memorials and in a Community Garden SO SUSTAINABILITY LA English DT Article DE stewardship; social resilience; community; urban green space; New York City ID URBAN ECOSYSTEM SERVICES; EXTREME EVENTS; PLACE; SENSE; SUSTAINABILITY; NETWORKS; INFRASTRUCTURE; ADAPTATION; GOVERNANCE; ATTACHMENT AB Resilience theory has received increased attention from researchers across a range of disciplines who have developed frameworks and articulated categories of indicators; however, there has been less discussion of how to recognize, and therefore support, social resilience at the community level, especially in urban areas. The value of urban environmental stewardship for supporting social-ecological functioning and improving quality of life in cities has been documented, but recognizing it as a strategy for strengthening social resilience to respond to future disturbances has not been fully explored. Here we address the question: How can social resilience indicators be operationalized as stewardship practices in an urban context? Using a deductive coding approach drawing upon existing resilience frameworks we analyze qualitative data from community managed-open spaces in the New York City area that have responded to various chronic presses and acute disturbances including a hurricane and a terrorist attack. In each case we identify and characterize the type of grounded, empirically observable stewardship practices that demonstrate the following indicators of social resilience at the community level: place attachment, social cohesion, social networks, and knowledge exchange and diversification. The process of operationalizing abstract indicators of social resilience has important implications for managers to support social (and ecological) resilience in the specific areas where stewardship takes place, as well as potentially having greater effects that bridge beyond the spatial and temporal boundaries of the site. We conclude by suggesting how researchers and practitioners might learn from our examples so they can recognize resilience in other sites in order to both inform research frameworks and strengthen practice and programming, while keeping larger institutional structures and context in mind. C1 [McMillen, Heather; Campbell, Lindsay K.; Svendsen, Erika S.; Reynolds, Renae] US Forest Serv, NYC Urban Field Stn, Bayside, NY 11359 USA. RP McMillen, H (reprint author), US Forest Serv, NYC Urban Field Stn, Bayside, NY 11359 USA. EM hmcmillen@fs.fed.us; lindsaycampbell@fs.fed.us; esvendsen@fs.fed.us; renaereynolds.ufs@gmail.com FU USDA Forest Service; TKF Foundation, Nature Sacred National Awards program FX The Living Memorials Project was supported by the USDA Forest Service, at the request of Congress. The Beach 41st Street project was supported in part by a grant from the TKF Foundation, as part of the Nature Sacred National Awards program. Thanks to our team members Victoria Marshall, Craig Desmond, Elizabeth Gilchrist, Heidi Woolever, Kira Appelhans, and Carmen Bouyer. Thanks to the NYCHA Garden and Greening Program, the Resident Green Committees, and Lee Trotman. We would like to acknowledge our Landscapes of Resilience collaborators Keith Tidball, Traci Sooter, Nancy Chikaraishi, and Nancy Sonti. We thank our research participants for sharing their time, insights, and inspiration. Finally, we thank three anonymous reviewers who helped improve the clarity of this paper. NR 84 TC 1 Z9 1 U1 22 U2 22 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD AUG PY 2016 VL 8 IS 8 AR 775 DI 10.3390/su8080775 PG 26 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA DU8HC UT WOS:000382452900075 ER PT J AU Raybon, H Schimleck, L Love, K Antony, F Sanders, J Daniels, R Andrews, E Schilling, E AF Raybon, Heath Schimleck, Laurence Love, Kim Antony, Finto Sanders, Joe Daniels, Richard Andrews, Edward Schilling, Erik TI Examination of the potential to reduce water application rates for hardwood pulp logs stored in wet decks SO TAPPI JOURNAL LA English DT Article ID TIME-DOMAIN REFLECTOMETRY; MOISTURE-CONTENT; PINE; STORAGE; TREES; STEM AB Wet storage of logs under sprinklers is often used to maintain log quality and to provide consistent fiber supply to wood production facilities. Concerns about water use in the southeastern United States have increased interest in refining water application strategies in woodyards. By understanding how the moisture content of stored logs varies over time in response to varying rates of water application, an optimum moisture regime for stored logs could be identified. In this study, experimental trials with nominal water application (100 mm/day) and a 30% reduction in water application were established at two hardwood woodyards in Georgia (Offerman, with sweet-gum and yellow poplar, and McBean, with red oak and sweetgum). Variations in log moisture were monitored using time domain reflectometry for 12 months at Offerman and 15 months at McBean. Significant differences between treatments were observed initially, likely resulting from pre-existing differences in the wood before the start of the experiment, but differences in log moisture soon disappeared. Pulping trials conducted using McBean woodyard logs stored for 9, 12, and 15 months found that treatment had no effect on pulp yield, indicating that a 30% reduction in the amount of water applied results in little change in log quality. C1 [Raybon, Heath; Antony, Finto; Sanders, Joe; Daniels, Richard] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Love, Kim] Univ Georgia, Ctr Stat Consulting, Athens, GA 30602 USA. [Andrews, Edward] US Forest Serv, USDA, Southern Res Stn, Athens, GA USA. [Schilling, Erik] NCASI Southern Reg Ctr, Newberry, FL USA. [Schimleck, Laurence] Oregon State Univ, Coll Forestry, Dept Wood Sci & Engn, Corvallis, OR 97331 USA. RP Schimleck, L (reprint author), Oregon State Univ, Coll Forestry, Dept Wood Sci & Engn, Corvallis, OR 97331 USA. EM Laurence.Schimleck@oregonstate.edu FU National Council for Air and Stream Improvement (NCASI); Wood Quality Consortium of the University of Georgia FX This research was supported by the National Council for Air and Stream Improvement (NCASI). The authors also gratefully acknowledge support from the Wood Quality Consortium of the University of Georgia, and to International Paper (McBean) and Rayonier (Offerman) for allowing access to their woodyards. NR 11 TC 0 Z9 0 U1 0 U2 0 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD AUG PY 2016 VL 15 IS 8 BP 523 EP 530 PG 8 WC Materials Science, Paper & Wood SC Materials Science GA DU9HH UT WOS:000382526800003 ER PT J AU Greuter, W Turland, NJ Wiersema, JH AF Greuter, Werner Turland, Nicholas J. Wiersema, John H. TI A proposal relating to infraspecific names (Article 24) SO TAXON LA English DT Editorial Material C1 [Greuter, Werner; Turland, Nicholas J.] Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Orto Bot, Herbarium Mediterraneum, Via Lincoln 2-A, I-90133 Palermo, Italy. [Wiersema, John H.] USDA ARS, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. RP Greuter, W (reprint author), Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany.; Greuter, W (reprint author), Orto Bot, Herbarium Mediterraneum, Via Lincoln 2-A, I-90133 Palermo, Italy. EM w.greuter@bgbm.org NR 5 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD AUG PY 2016 VL 65 IS 4 BP 905 EP 906 DI 10.12705/654.36 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DU7VT UT WOS:000382423400036 ER PT J AU Greuter, W Wiersema, JH Turland, NJ AF Greuter, Werner Wiersema, John H. Turland, Nicholas J. TI (334-336) Proposals relating to the valid publication of new combinations, names at new rank, and replacement names (Article 41) SO TAXON LA English DT Editorial Material C1 [Greuter, Werner; Turland, Nicholas J.] Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany. [Greuter, Werner] Orto Bot, Herbarium Mediterraneum, Via Lincoln 2-A, I-90133 Palermo, Italy. [Wiersema, John H.] ARS, USDA, Natl Germplasm Resources Lab, Beltsville Agr Res Ctr BARC West, Bldg 003, Beltsville, MD 20705 USA. RP Greuter, W (reprint author), Bot Garten & Bot Museum Berlin, Konigin Luise Str 6-8, D-14195 Berlin, Germany.; Greuter, W (reprint author), Orto Bot, Herbarium Mediterraneum, Via Lincoln 2-A, I-90133 Palermo, Italy. EM w.greuter@bgbnz.org NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD AUG PY 2016 VL 65 IS 4 BP 911 EP 912 DI 10.12705/654.41 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA DU7VT UT WOS:000382423400041 ER PT J AU Grenier, B Dohnal, I Shanmugasundaram, R Eicher, SD Selvaraj, RK Schatzmayr, G Applegate, TJ AF Grenier, Bertrand Dohnal, Ilse Shanmugasundaram, Revathi Eicher, Susan D. Selvaraj, Ramesh K. Schatzmayr, Gerd Applegate, Todd J. TI Susceptibility of Broiler Chickens to Coccidiosis When Fed Subclinical Doses of Deoxynivalenol and Fumonisins-Special Emphasis on the Immunological Response and the Mycotoxin Interaction SO TOXINS LA English DT Article DE mycotoxins; coccidiosis; challenge; interaction; intestinal immune response ID INTESTINAL IMMUNE-RESPONSES; FUSARIUM MYCOTOXINS; CULTURE MATERIAL; FEED; B-1; EIMERIA; METAANALYSIS; PERFORMANCE; CHALLENGE; TOXICITY AB Deoxynivalenol (DON) and fumonisins (FB) are the most frequently encountered mycotoxins produced by Fusarium species in livestock diets. The effect of subclinical doses of mycotoxins in chickens is largely unknown, and in particular the susceptibility of birds to pathogenic challenge when fed these fungal metabolites. Therefore, the present study reports the effects of DON and FB on chickens challenged with Eimeria spp, responsible for coccidiosis. Broilers were fed diets from hatch to day 20, containing no mycotoxins, 1.5 mg DON/kg, 20 mg FB/kg, or both toxins (12 pens/diet; 7 birds/pen). At day 14, six pens of birds per diet (half of the birds) were challenged with a 25 x-recommended dose of coccidial vaccine, and all birds (challenged and unchallenged) were sampled 6 days later. As expected, performance of birds was strongly affected by the coccidial challenge. Ingestion of mycotoxins did not further affect the growth but repartitioned the rate of reduction (between the fraction due to the change in maintenance and feed efficiency), and reduced apparent nitrogen digestibility. Intestinal lesions and number of oocysts in the jejunal mucosa and feces of challenged birds were more frequent and intense in the birds fed mycotoxins than in birds fed control feed. The upregulation of cytokines (interleukin (IL) IL-1 beta, IL-6, IL-8 and IL-10) following coccidial infection was higher in the jejunum of birds fed mycotoxins. Further, the higher intestinal immune response was associated with a higher percentage of T lymphocytes CD4(+)CD25(+), also called T-regs, observed in the cecal tonsils of challenged birds fed mycotoxins. Interestingly, the increase in FB biomarker of exposure (sphinganine/sphingosine ratio in serum and liver) suggested a higher absorption and bioavailability of FB in challenged birds. The interaction of DON and FB was very dependent on the endpoint assessed, with three endpoints reporting antagonism, nine additivity, and two synergism. In conclusion, subclinical doses of DON and FB showed little effects in unchallenged chickens, but seem to result in metabolic and immunologic disturbances that amplify the severity of coccidiosis. C1 [Grenier, Bertrand; Applegate, Todd J.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Grenier, Bertrand; Dohnal, Ilse; Schatzmayr, Gerd] BIOMIN Res Ctr, A-3430 Tulln, Austria. [Shanmugasundaram, Revathi; Selvaraj, Ramesh K.] Ohio Agr Res & Dev Ctr, Dept Anim Sci, Wooster, OH 44691 USA. [Eicher, Susan D.] ARS, Livestock Behav Res Unit, USDA, W Lafayette, IN 47907 USA. [Applegate, Todd J.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. RP Applegate, TJ (reprint author), Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA.; Applegate, TJ (reprint author), Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. EM bertrand.grenier@biomin.net; ilse.dohnal@biomin.net; shanmugasundaram.2@osu.edu; Susan.Eicher@ars.usda.gov; selvaraj.7@osu.edu; gerd.schatzmayr@biomin.net; applegt@uga.edu OI Eicher, Susan/0000-0001-5832-4049; Grenier, Bertrand/0000-0002-8889-7240; Applegate, Todd/0000-0002-2406-5058 FU BIOMIN FX Bertrand Grenier is supported by a post-doctoral fellowship financed by BIOMIN. The authors thank Heidi-Elisabeth Schwartz-Zimmermann for advice on Sa/So analysis, and Petra Mayrhofer for performing the Sa/So sample preparation. In addition, we are grateful to Pat Jaynes (Dept. of Animal Sciences, Purdue University) for her help with coordinating digestibility and histological analyses. NR 48 TC 0 Z9 0 U1 11 U2 11 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD AUG PY 2016 VL 8 IS 8 AR 231 DI 10.3390/toxins8080231 PG 22 WC Toxicology SC Toxicology GA DU5RK UT WOS:000382268800005 ER PT J AU Wilson, WC Ruder, MG Jasperson, D Smith, TPL Naraghi-Arani, P Lenhoff, R Stallknecht, DE Valdivia-Granda, WA Sheoran, D AF Wilson, W. C. Ruder, M. G. Jasperson, D. Smith, T. P. L. Naraghi-Arani, P. Lenhoff, R. Stallknecht, D. E. Valdivia-Granda, W. A. Sheoran, D. TI Molecular evolution of epizootic hemorrhagic disease viruses in North America based on historical isolates using motif fingerprints SO VIRUS GENES LA English DT Article DE Epizootic hemorrhagic disease; Molecular evolution; Motif fingerprint ID WHITE-TAILED DEER; BLUETONGUE-VIRUS; SEQUENCE-ANALYSIS; UNITED-STATES; ODOCOILEUS-VIRGINIANUS; PHYLOGENETIC ANALYSIS; STRAINS; GENE; GENOME; CULICOIDES AB Epizootic hemorrhagic disease virus (EHDV) is an orbivirus of the Reoviridae family that has significant impact on wild and captive white-tailed deer. Although closely related to bluetongue virus that can cause disease in sheep and cattle, North American EHDV historically has not been associated with disease in cattle or sheep. Severe disease in cattle has been reported with other EHDV strains from East Asia and the Middle East. To understand the potential role of viral genetics in the epidemiology of epizootic hemorrhagic disease, a molecular characterization of North American EHDV strains from 1955 to 2012 was conducted via conventional phylogenetic analysis and a new classification approach using motif fingerprint patterns. Overall, this study indicates that the genetic make-up of EHDV populations in North America have slowly evolved over time. The data also suggested limited reassortment events between serotypes 1 and 2 and introduces a new analysis tool for more detailed sequence pattern analysis. C1 [Wilson, W. C.; Ruder, M. G.; Jasperson, D.] USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. [Smith, T. P. L.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Naraghi-Arani, P.; Lenhoff, R.] Lawrence Livermore Natl Lab, Assay Dev & Virol, Livermore, CA USA. [Ruder, M. G.; Stallknecht, D. E.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, Southeastern Cooperat Wildlife Dis Study, Athens, GA USA. [Valdivia-Granda, W. A.; Sheoran, D.] Or Integrated Biosci Inc, New Rochelle, NY USA. [Naraghi-Arani, P.] InSilixa Inc, Sunnyvale, CA USA. [Lenhoff, R.] Cerus Corp, Concord, CA USA. RP Wilson, WC (reprint author), USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM william.wilson@ars.usda.gov FU United States Department of Agriculture (USDA), Agricultural Research Service [3020-32000-006-00D]; U.S. Department of Homeland Security (DHS) [DHS-2010-ST-061-AG0001] FX The authors thank Drs. Jonathan Breitenbach and Ben M. Hause for early review of this manuscript, and Mr. Bob Lee for technical assistance. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the USDA or DHS. USDA is an equal opportunity provider and employer. The United States Department of Agriculture (USDA), Agricultural Research Service (Project #3020-32000-006-00D), supported this work. The U.S. Department of Homeland Security (DHS) under Grant Award Number DHS-2010-ST-061-AG0001 supports W. Valdivia-Granda. NR 28 TC 0 Z9 0 U1 6 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD AUG PY 2016 VL 52 IS 4 BP 495 EP 508 DI 10.1007/s11262-016-1332-z PG 14 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA DV0UO UT WOS:000382636200007 PM 27107856 ER PT J AU Han, JY Chung, J Kim, J Seo, EY Kilcrease, JP Bauchan, GR Lim, S Hammond, J Lim, HS AF Han, Jae-Yeong Chung, Jinsoo Kim, Jungkyu Seo, Eun-Young Kilcrease, James P. Bauchan, Gary R. Lim, Seungmo Hammond, John Lim, Hyoun-Sub TI Comparison of helper component-protease RNA silencing suppression activity, subcellular localization, and aggregation of three Korean isolates of Turnip mosaic virus SO VIRUS GENES LA English DT Article DE Turnip mosaic virus; Helper component-protease (HC-Pro); RNA silencing suppressor efficiency; Pathogenicity ID PLUM-POX-VIRUS; HC-PRO; MULTIFUNCTIONAL PROTEIN; COAT PROTEIN; APHID TRANSMISSION; SYMPTOM EXPRESSION; POTYVIRUS; GENE; MUTATIONS; SEQUENCE AB In 2014, we performed a nationwide survey in Korean radish fields to investigate the distribution and variability of Turnip mosaic virus (TuMV). Brassica rapa ssp. pekinensis sap-inoculated with three isolates of TuMV from infected radish tissue showed different symptom severities, whereas symptoms in Raphanus sativus were similar for each isolate. The helper component-protease (HC-Pro) genes of each isolate were sequenced, and phylogenetic analysis showed that the three Korean isolates were clustered into the basal-BR group. The HC-Pro proteins of these isolates were tested for their RNA silencing suppressor (VSR) activity and subcellular localization in Nicotiana benthamiana. A VSR assay by co-agroinfiltration of HC-Pro with soluble-modified GFP (smGFP) showed that HC-Pro of isolate R007 and R041 showed stronger VSR activity than R065. The HC-Pros showed 98.25 % amino acid identity, and weak VSR isolate (R065) has a single variant residue in the C-terminal domain associated with protease activity and self-interaction compared to isolates with strong VSR activity. Formation of large subcellular aggregates of GFP: HC-Pro fusion proteins in N. benthamiana was only observed for HC-Pro from isolates with strong VSR activity, suggesting that R065 'weak' HC-Pro may have diminished self-association; substitution of the variant C-terminal residue largely reversed the HC-Pro aggregation and silencing suppressor characteristics. The lack of correlation between VSR efficiency and induction of systemic necrosis (SN) suggests that differences in viral accumulation due to HC-Pro are not responsible for SN. C1 [Han, Jae-Yeong; Chung, Jinsoo; Kim, Jungkyu; Seo, Eun-Young; Lim, Hyoun-Sub] Chungnam Natl Univ, Daejeon, South Korea. [Kilcrease, James P.; Hammond, John] USDA ARS, Floral & Nursery Plants Res Unit, USNA, Beltsville, MD 20705 USA. [Bauchan, Gary R.] USDA ARS, Electron & Confocal Microscopy Unit, BARC, Beltsville, MD 20705 USA. [Lim, Seungmo] Korea Res Inst Biosci & Biotechnol, Plant Syst Engn Res Ctr, Daejeon 305806, South Korea. [Lim, Seungmo] Univ Sci & Technol, Biosyst & Bioengn Program, Daejeon 305350, South Korea. RP Lim, HS (reprint author), Chungnam Natl Univ, Daejeon, South Korea.; Hammond, J (reprint author), USDA ARS, Floral & Nursery Plants Res Unit, USNA, Beltsville, MD 20705 USA. EM john.hammond@ars.usda.gov; hyounlim@cnu.ac.kr FU Golden Seed Project Vegetable Seed Center, Ministry of Agriculture, Food and Rural Affairs, Korea [213002-04-2-WTc11] FX This research was supported by Golden Seed Project Vegetable Seed Center (213002-04-2-WTc11), Ministry of Agriculture, Food and Rural Affairs, Korea. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture (USDA); USDA is an equal opportunity provider and employer. NR 44 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0920-8569 EI 1572-994X J9 VIRUS GENES JI Virus Genes PD AUG PY 2016 VL 52 IS 4 BP 592 EP 596 DI 10.1007/s11262-016-1330-1 PG 5 WC Genetics & Heredity; Virology SC Genetics & Heredity; Virology GA DV0UO UT WOS:000382636200021 PM 27059238 ER PT J AU Lokupitiya, E Denning, AS Schaefer, K Ricciuto, D Anderson, R Arain, MA Baker, I Barr, AG Chen, G Chen, JM Ciais, P Cook, DR Dietze, M El Maayar, M Fischer, M Grant, R Hollinger, D Izaurralde, C Jain, A Kucharik, C Li, Z Liu, S Li, L Matamala, R Peylin, P Price, D Running, SW Sahoo, A Sprintsin, M Suyker, AE Tian, H Tonitto, C Torn, M Verbeeck, H Verma, SB Xue, Y AF Lokupitiya, E. Denning, A. S. Schaefer, K. Ricciuto, D. Anderson, R. Arain, M. A. Baker, I. Barr, A. G. Chen, G. Chen, J. M. Ciais, P. Cook, D. R. Dietze, M. El Maayar, M. Fischer, M. Grant, R. Hollinger, D. Izaurralde, C. Jain, A. Kucharik, C. Li, Z. Liu, S. Li, L. Matamala, R. Peylin, P. Price, D. Running, S. W. Sahoo, A. Sprintsin, M. Suyker, A. E. Tian, H. Tonitto, C. Torn, M. Verbeeck, Hans Verma, S. B. Xue, Y. TI Carbon and energy fluxes in cropland ecosystems: a model-data comparison SO BIOGEOCHEMISTRY LA English DT Article DE Carbon and energy fluxes; Cropland ecosystems; Land-atmosphere exchange; Model-data comparison; Cropland carbon and energy exchange ID RAIN-FED MAIZE; BIOSPHERE MODEL; VEGETATION DYNAMICS; DIOXIDE EXCHANGE; BALANCE CLOSURE; CLIMATE-CHANGE; WATER BUDGETS; CO2; FOREST; SOIL AB Croplands are highly productive ecosystems that contribute to land-atmosphere exchange of carbon, energy, and water during their short growing seasons. We evaluated and compared net ecosystem exchange (NEE), latent heat flux (LE), and sensible heat flux (H) simulated by a suite of ecosystem models at five agricultural eddy covariance flux tower sites in the central United States as part of the North American Carbon Program Site Synthesis project. Most of the models overestimated H and underestimated LE during the growing season, leading to overall higher Bowen ratios compared to the observations. Most models systematically under predicted NEE, especially at rain-fed sites. Certain crop-specific models that were developed considering the high productivity and associated physiological changes in specific crops better predicted the NEE and LE at both rain-fed and irrigated sites. Models with specific parameterization for different crops better simulated the inter-annual variability of NEE for maize-soybean rotation compared to those models with a single generic crop type. Stratification according to basic model formulation and phenological methodology did not explain significant variation in model performance across these sites and crops. The under prediction of NEE and LE and over prediction of H by most of the models suggests that models developed and parameterized for natural ecosystems cannot accurately predict the more robust physiology of highly bred and intensively managed crop ecosystems. When coupled in Earth System Models, it is likely that the excessive physiological stress simulated in many land surface component models leads to overestimation of temperature and atmospheric boundary layer depth, and underestimation of humidity and CO2 seasonal uptake over agricultural regions. C1 [Lokupitiya, E.] Univ Colombo, Dept Zool & Environm Sci, Fac Sci, Colombo 03, Sri Lanka. [Denning, A. S.; Baker, I.] Colorado State Univ, Dept Atmospher Sci, Ft Collins, CO 80523 USA. [Schaefer, K.] Univ Colorado, NSIDC, Boulder, CO 80309 USA. [Ricciuto, D.] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Anderson, R.; Running, S. W.] Univ Montana, Numer Terradynam Simulat Grp, Missoula, MT 59812 USA. [Arain, M. A.] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON L8S 4K1, Canada. [Arain, M. A.] McMaster Univ, McMaster Ctr Climate Change, Hamilton, ON L8S 4K1, Canada. [Barr, A. G.] Environm Canada, Sci & Technol Branch, Natl Hydrol Res Ctr, Innovat Blvd, Saskatoon, SK S7N 3H5, Canada. [Chen, G.; Tian, H.] Auburn Univ, Sch Forestry & Wildlife Sci, Ecosyst Dynam & Global Ecol Lab, Auburn, AL 36849 USA. [Chen, J. M.] Univ Toronto, Dept Geog, Toronto, ON M5S 3G3, Canada. [Ciais, P.; Peylin, P.] UVSQ, CNRS, CEA, LSCE, F-91191 Gif Sur Yvette, France. [Cook, D. R.; Matamala, R.] Argonne Natl Lab, Div Environm Sci, Lemont, IL 60439 USA. [Dietze, M.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [El Maayar, M.] Cyprus Inst, Energy Environm & Water Res Ctr, Nicosia, Cyprus. [Fischer, M.; Torn, M.] Lawrence Berkley Natl Lab, Berkeley, CA 94718 USA. [Grant, R.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2E3, Canada. [Hollinger, D.] US Forest Serv, Northern Res Stn, USDA, Durham, NH 03824 USA. [Izaurralde, C.] Pacific Northwest Natl Lab, College Pk, MD 20740 USA. [Izaurralde, C.] Univ Maryland, College Pk, MD 20740 USA. [Jain, A.] Univ Illinois, Dept Atmospher Sci, Urbana, IL 61801 USA. [Kucharik, C.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Kucharik, C.] Univ Wisconsin, Nelson Inst Ctr Sustainabil & Global Environm, Madison, WI 53706 USA. [Li, Z.] Teleobservat Res LLC, Columbia, MD 21044 USA. [Liu, S.] US Geol Survey, Earth Resources Observat & Sci EROS Ctr, Sioux Falls, SD 57198 USA. [Li, L.] Univ Technol Sydney, Sch Life Sci, POB 123, Broadway, NSW 2007, Australia. [Price, D.] Nat Resources Canada, Northern Forestry Ctr, 5320-120 St, Edmonton, AB T6H3S5, Canada. [Sahoo, A.] Princeton Univ, Dept Civil & Environm Engn, E324 Engn Quad, Princeton, NJ 08544 USA. [Sprintsin, M.] Jewish Natl Fund Keren Kayemet LeIsrael, Forest Management & GIS Dept, Jerusalem, Israel. [Suyker, A. E.; Verma, S. B.] Univ Nebraska, Sch Nat Resources, 807 Hardin Hall, Lincoln, NE 68583 USA. [Tonitto, C.] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA. [Verbeeck, Hans] Univ Ghent, Fac Biosci Engn, CAVElab Computat & Appl Vegetat Ecol, B-9000 Ghent, Belgium. [Xue, Y.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90095 USA. RP Lokupitiya, E (reprint author), Univ Colombo, Dept Zool & Environm Sci, Fac Sci, Colombo 03, Sri Lanka. EM erandi@sci.cmb.ac.lk RI Torn, Margaret/D-2305-2015; Ricciuto, Daniel/I-3659-2016; Jain, Atul/D-2851-2016; OI Ricciuto, Daniel/0000-0002-3668-3021; Jain, Atul/0000-0002-4051-3228; Kucharik, Christopher/0000-0002-0400-758X FU U.S. Department of Energy (DoE) [DE-FG02-06ER64317, DE-AC02-05CH11231]; National Oceanic and Atmospheric Administration [NA07OAR4310115]; Office of Biological and Environmental Research of the U.S. Department of Energy [DE-AC02-05CH11231]; Center for Multiscale Modeling of Atmospheric Processes (CMMAP) [NSF-ATM-0425247] FX We would like to thank the North American Carbon Program Site-Level Interim Synthesis team, the Modeling and Synthesis Thematic Data Center, and the Oak Ridge National Laboratory Distributed Active Archive Center for collecting, organizing, and distributing the model output and flux observations required for this analysis. We acknowledge the comments given by Dr. Andrew Richardson during the initial stages of this manuscript. This research was partly funded by the U.S. Department of Energy (DoE; under contract Nos DE-FG02-06ER64317 and DE-AC02-05CH11231) and National Oceanic and Atmospheric Administration Award NA07OAR4310115. Data from the US-ARM site was supported by the Office of Biological and Environmental Research of the U.S. Department of Energy (under grant or contract DE-AC02-05CH11231) as part of the Atmospheric Radiation Measurement Program. We also acknowledge the support from the Center for Multiscale Modeling of Atmospheric Processes (CMMAP; NSF-ATM-0425247). Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 72 TC 0 Z9 0 U1 11 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD AUG PY 2016 VL 129 IS 1-2 BP 53 EP 76 DI 10.1007/s10533-016-0219-3 PG 24 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA DT2BY UT WOS:000381287000004 ER PT J AU Wechter, WP McMillan, MM Keinath, AP Smith, JP Farnham, MW AF Wechter, W. Patrick McMillan, Melanie M. Keinath, Anthony P. Smith, J. Powell Farnham, Mark W. TI 'Carolina Broadleaf' Mustard Green (Brassica juncea) Resistant to the Bacterial Leaf Blight Pathogen Pseudomonas cannabina pv. alisalensis SO HORTSCIENCE LA English DT Article DE Brassica juncea; leafy-green; open pollinated; host plant resistance ID 1ST REPORT; CALIFORNIA; ARUGULA C1 [Wechter, W. Patrick; McMillan, Melanie M.; Farnham, Mark W.] ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. [Keinath, Anthony P.] Clemson Univ, Coastal Res & Educ Ctr, 2700 Savannah Highway, Charleston, SC 29414 USA. [Smith, J. Powell] Clemson Univ, Cooperat Extens Serv, 605 West Main St,Suite 109, Lexington, SC 29072 USA. RP Wechter, WP (reprint author), ARS, USDA, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM pat.wechter@ars.usda.gov NR 11 TC 0 Z9 0 U1 4 U2 4 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2016 VL 51 IS 8 BP 1062 EP 1064 PG 3 WC Horticulture SC Agriculture GA DU7RK UT WOS:000382412100017 ER PT J AU Wechter, WP McMillan, MM Farnham, MW Levi, A AF Wechter, W. Patrick McMillan, Melanie M. Farnham, Mark W. Levi, Amnon TI Watermelon Germplasm Lines. USVL246-FR2 and USVL252-FR2 Tolerant to Fusarium oxysporum f. sp niveum Race 2 SO HORTSCIENCE LA English DT Article DE Citrullus citroides; inbred line; host plant resistance ID UNITED-STATES; WILT; RESISTANCE; 1ST-REPORT; MARYLAND; DELAWARE C1 [Wechter, W. Patrick; McMillan, Melanie M.; Farnham, Mark W.; Levi, Amnon] ARS, US Vegetable Lab, USDA, 2700 Savannah Highway, Charleston, SC 29414 USA. RP Wechter, WP (reprint author), ARS, US Vegetable Lab, USDA, 2700 Savannah Highway, Charleston, SC 29414 USA. EM pat.wechter@ars.usda.gov NR 16 TC 1 Z9 1 U1 5 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2016 VL 51 IS 8 BP 1065 EP 1067 PG 3 WC Horticulture SC Agriculture GA DU7RK UT WOS:000382412100018 ER PT J AU Price, NPJ Labeda, DP Naumann, TA Vermillion, KE Bowman, MJ Berhow, MA Metcalf, WW Bischoff, KM AF Price, Neil P. J. Labeda, David P. Naumann, Todd A. Vermillion, Karl E. Bowman, Michael J. Berhow, Mark A. Metcalf, William W. Bischoff, Kenneth M. TI Quinovosamycins: new tunicamycin-type antibiotics in which the alpha, beta-1 '' 11 '-linked N-acetylglucosamine residue is replaced by N-acetylquinovosamine SO JOURNAL OF ANTIBIOTICS LA English DT Article ID EXPLORATORY CARBOHYDRATE-CHEMISTRY; ASPARAGINE-LINKED GLYCOSYLATION; PSEUDOMONAS-AERUGINOSA; BIOSYNTHETIC PATHWAYS; NUCLEOSIDE ANTIBIOTICS; ESCHERICHIA-COLI; NATURAL-PRODUCTS; TEICHOIC-ACID; O-ANTIGEN; WALL AB Tunicamycins (TUN) are potent inhibitors of polyprenyl phosphate N-acetylhexosamine 1-phosphate transferases (PPHP), including essential eukaryotic GPT enzymes and bacterial HexNAc 1-P translocases. Hence, TUN blocks the formation of eukaryotic N-glycoproteins and the assembly of bacterial call wall polysaccharides. The genetic requirement for TUN production is well-established. Using two genes unique to the TUN pathway (tunB and tunD) as probes we identified four new prospective TUN-producing strains. Chemical analysis showed that one strain, Streptomyces niger NRRL B-3857, produces TUN plus new compounds, named quinovosamycins (QVMs). QVMs are structurally akin to TUN, but uniquely in the 1 '',11'-HexNAc sugar head group, which is invariably D-GlcNAc for the known TUN, but is D-QuiNAc for the QVM. Surprisingly, this modification has only a minor effect on either the inhibitory or antimicrobial properties of QVM and TUN. These findings have unexpected consequences for TUN/QVM biosynthesis, and for the specificity of the PPHP enzyme family. C1 [Price, Neil P. J.; Bischoff, Kenneth M.] USDA ARS, Renewable Prod Technol Unit, Natl Ctr Agr Utilizat Res, 1815N Univ St, Peoria, IL 61604 USA. [Labeda, David P.; Naumann, Todd A.] USDA ARS, Mycotoxin Prevent & Appl Microbiol Unit, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Vermillion, Karl E.; Berhow, Mark A.] USDA ARS, Funct Foods Unit, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Bowman, Michael J.] USDA ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Metcalf, William W.] Univ Illinois, Dept Microbiol, Urbana, IL USA. [Metcalf, William W.] Univ Illinois, Inst Genom Biol, Urbana, IL USA. RP Price, NPJ (reprint author), USDA ARS, Renewable Prod Technol Unit, Natl Ctr Agr Utilizat Res, 1815N Univ St, Peoria, IL 61604 USA. EM neil.price@ars.usda.gov NR 52 TC 0 Z9 0 U1 3 U2 3 PU JAPAN ANTIBIOTICS RESEARCH ASSOC PI TOKYO PA 2 20 8 KAMIOSAKI SHINAGAWA KU, TOKYO, 141, JAPAN SN 0021-8820 J9 J ANTIBIOT JI J. Antibiot. PD AUG PY 2016 VL 69 IS 8 SI SI BP 637 EP 646 DI 10.1038/ja.2016.49 PG 10 WC Biotechnology & Applied Microbiology; Immunology; Microbiology; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Immunology; Microbiology; Pharmacology & Pharmacy GA DU3GS UT WOS:000382099400012 PM 27189123 ER PT J AU Gutierrez-Carbonell, E Takahashi, D Luthje, S Gonzalez-Reyes, JA Mongrand, S Contreras-Moreira, B Abadia, A Uemura, M Abadia, J Lopez-Millan, AF AF Gutierrez-Carbonell, Elain Takahashi, Daisuke Luethje, Sabine Antonio Gonzalez-Reyes, Jose Mongrand, Sebastien Contreras-Moreira, Bruno Abadia, Anunciacion Uemura, Matsuo Abadia, Javier Lopez-Millan, Ana Flor TI A Shotgun Proteomic Approach Reveals That Fe Deficiency Causes Marked Changes in the Protein Profiles of Plasma Membrane and Detergent-Resistant Microdomain Preparations from Beta vulgaris Roots SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE Detergent-resistant microdomain; Iron deficiency; Lipidomics; Plasma membrane; Proteomics; Sugar beet ID FERRIC-CHELATE REDUCTASE; IRON-DEFICIENCY; ARABIDOPSIS ROOTS; SUGAR-BEET; MEDICAGO-TRUNCATULA; TRANSPORTER GENES; QUINCE GENOTYPES; OXIDATIVE STRESS; PLANTS; RESPONSES AB In the present study we have used label-free shotgun proteomic analysis to examine the effects of Fe deficiency on the protein profiles of highly pure sugar beet root plasma membrane (PM) preparations and detergent-resistant membranes (DRMs), the latter as an approach to study microdomains. Altogether, 545 proteins were detected, with 52 and 68 of them changing significantly with Fe deficiency in PM and DRM, respectively. Functional categorization of these proteins showed that signaling and general and vesicle related transport accounted for approximately 50% of the differences in both PM and DRM, indicating that from a qualitative point of view changes induced by Fe deficiency are similar in both preparations. Results indicate that Fe deficiency has an impact in phosphorylation processes at the PM level and highlight the involvement of signaling proteins, especially those from the 14-3-3 family. Lipid profiling revealed Fe-deficiency induced decreases in phosphatidic acid derivatives, which may impair vesicle formation, in agreement with the decreases measured in proteins related to intracellular trafficking and secretion. The modifications induced by Fe deficiency in the relative enrichment of proteins in DRMs revealed the existence of a group of cytoplasmic proteins that appears to be more attached to the PM in conditions of Fe deficiency. C1 [Gutierrez-Carbonell, Elain; Abadia, Anunciacion; Abadia, Javier] CSIC, Plant Nutr Dept, Plant Stress Physiol Grp, Apdo 13034, E-50080 Zaragoza, Spain. [Contreras-Moreira, Bruno] CSIC, Aula Dei Expt Stn, Lab Computat & Struct Biol, Apdo 13034, E-50080 Zaragoza, Spain. [Takahashi, Daisuke] Iwate Univ, Fac Agr, United Grad Sch Agr Sci, Morioka, Iwate 0208550, Japan. [Takahashi, Daisuke] Iwate Univ, Fac Agr, Cryobiofrontier Res Ctr, Morioka, Iwate 0208550, Japan. [Luethje, Sabine] Univ Hamburg, Bioctr Klein Flottbek, Ohnhorststr 18, D-22609 Hamburg, Germany. [Antonio Gonzalez-Reyes, Jose] Univ Cordoba, Dept Biol Celular Fisiol & Inmunol, Campus Rabanales,Edificio Severo Ochoa, Cordoba 14014, Spain. [Mongrand, Sebastien] CNRS Univ Bordeaux Segalen, UMR 5200, Lab Biogenese Membranaire, INRA Bordeaux Aquitaine, Batiment A3,71 Rue Edouard Borlaux,CS 20032, F-33140 Villenave Dornon, France. [Contreras-Moreira, Bruno] Fdn ARAID, Calle Maria de Luna 11, Zaragoza 50018, Spain. [Lopez-Millan, Ana Flor] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. [Gutierrez-Carbonell, Elain] Fibrostatin SL Univ Valencia, Sci Pk,Catedrat Agustin Escardino 9, Valencia 46980, Spain. [Takahashi, Daisuke] Max Planck Inst Mol Plant Physiol, Cent Infrastruct Grp Genom & Transcript Profil, Muhlenberg 1, D-14476 Potsdam, Germany. RP Lopez-Millan, AF (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM lopezmil@bcm.edu RI Abadia, Javier/B-8804-2008; Contreras-Moreira, Bruno/G-4334-2011; Mangan, Rachel/A-8824-2008; Abadia, Anunciacion/A-7474-2010; OI Abadia, Javier/0000-0001-5470-5901; Contreras-Moreira, Bruno/0000-0002-5462-907X; Mangan, Rachel/0000-0002-8788-9214; Abadia, Anunciacion/0000-0003-3609-7070; Gonzalez-Reyes, Jose A./0000-0003-1918-5490 FU Spanish Ministry of Economy and Competitivity (MINECO) [AGL2012-31988, AGL2013-42175-R]; FEDER; Aragon Government (Group A03); Japan Society for the Promotion of Science [24-7373, 22120003, 24370018]; Bordeaux Metabolome Facility-MetaboHUB [ANR-11-INBS-0010]; platform Metabolome-Lipidome-Fluxome of Bordeaux; JAE Pre-CSIC contract FX Supported by the Spanish Ministry of Economy and Competitivity (MINECO; projects AGL2012-31988, and AGL2013-42175-R, cofinanced with FEDER), and the Aragon Government (Group A03). Research conducted in Iwate University was in part supported by Grants-in-Aid for Scientific Research (#24-7373, #22120003, and #24370018) from the Japan Society for the Promotion of Science. Research conducted in Bordeaux was supported by Bordeaux Metabolome Facility-MetaboHUB (grant no. ANR-11-INBS-0010 to S.M.) and platform Metabolome-Lipidome-Fluxome of Bordeaux (contribution toward lipid analysis equipment). E.G.-C. was supported by a JAE Pre-CSIC contract. NR 77 TC 1 Z9 1 U1 13 U2 17 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 EI 1535-3907 J9 J PROTEOME RES JI J. Proteome Res. PD AUG PY 2016 VL 15 IS 8 BP 2510 EP 2524 DI 10.1021/acs.jproteome.6b00026 PG 15 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA DT1IW UT WOS:000381235900015 PM 27321140 ER PT J AU Marzano, SYL Nelson, BD Ajayi-Oyetunde, O Bradley, CA Hughes, TJ Hartman, GL Eastburn, DM Domier, LL AF Marzano, Shin-Yi Lee Nelson, Berlin D. Ajayi-Oyetunde, Olutoyosi Bradley, Carl A. Hughes, Teresa J. Hartman, Glen L. Eastburn, Darin M. Domier, Leslie L. TI Identification of Diverse Mycoviruses through Metatranscriptomics Characterization of the Viromes of Five Major Fungal Plant Pathogens SO JOURNAL OF VIROLOGY LA English DT Article ID COMPLETE NUCLEOTIDE-SEQUENCE; VIRUS-LIKE PARTICLES; YELLOW VEIN VIRUS; SCLEROTINIA-SCLEROTIORUM; MOLECULAR CHARACTERIZATION; CLOSTRIDIUM-PERFRINGENS; DNA MYCOVIRUS; COAT PROTEIN; RNA VIRUSES; TRANSMISSION AB Mycoviruses can have a marked effect on natural fungal communities and influence plant health and productivity. However, a comprehensive picture of mycoviral diversity is still lacking. To characterize the viromes of five widely dispersed plant-pathogenic fungi, Colletotrichum truncatum, Macrophomina phaseolina, Diaporthe longicolla, Rhizoctonia solani, and Sclerotinia sclerotiorum, a high-throughput sequencing-based metatranscriptomic approach was used to detect viral sequences. Total RNA and double-stranded RNA (dsRNA) from mycelia and RNA from samples enriched for virus particles were sequenced. Sequence data were assembled de novo, and contigs with predicted amino acid sequence similarities to viruses in the nonredundant protein database were selected. The analysis identified 72 partial or complete genome segments representing 66 previously undescribed mycoviruses. Using primers specific for each viral contig, at least one fungal isolate was identified that contained each virus. The novel mycoviruses showed affinity with 15 distinct lineages: Barnaviridae, Benyviridae, Chrysoviridae, Endornaviridae, Fusariviridae, Hypoviridae, Mononegavirales, Narnaviridae, Ophioviridae, Ourmiavirus, Partitiviridae, Tombusviridae, Totiviridae, Tymoviridae, and Virgaviridae. More than half of the viral sequences were predicted to be members of the Mitovirus genus in the family Narnaviridae, which replicate within mitochondria. Five viral sequences showed strong affinity with three families (Benyviridae, Ophioviridae, and Virgaviridae) that previously contained no mycovirus species. The genomic information provides insight into the diversity and taxonomy of mycoviruses and coevolution of mycoviruses and their fungal hosts. IMPORTANCE Plant-pathogenic fungi reduce crop yields, which affects food security worldwide. Plant host resistance is considered a sustainable disease management option but may often be incomplete or lacking for some crops to certain fungal pathogens or strains. In addition, the rising issues of fungicide resistance demand alternative strategies to reduce the negative impacts of fungal pathogens. Those fungus-infecting viruses (mycoviruses) that attenuate fungal virulence may be welcome additions for mitigation of plant diseases. By high-throughput sequencing of the RNAs from 275 isolates of five fungal plant pathogens, 66 previously undescribed mycoviruses were identified. In addition to identifying new potential biological control agents, these results expand the grand view of the diversity of mycoviruses and provide possible insights into the importance of intracellular and extracellular transmission in fungus-virus coevolution. C1 [Marzano, Shin-Yi Lee; Ajayi-Oyetunde, Olutoyosi; Bradley, Carl A.; Hartman, Glen L.; Eastburn, Darin M.; Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Nelson, Berlin D.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND USA. [Hughes, Teresa J.] Monsanto Co, Chesterfield, MO USA. [Hartman, Glen L.; Domier, Leslie L.] ARS, USDA, Urbana, IL 61801 USA. [Marzano, Shin-Yi Lee] South Dakota State Univ, Dept Plant Sci, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Bradley, Carl A.] Univ Kentucky, Res & Educ Ctr, Dept Plant Pathol, Princeton, KY USA. RP Marzano, SYL; Domier, LL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA.; Domier, LL (reprint author), ARS, USDA, Urbana, IL 61801 USA.; Marzano, SYL (reprint author), South Dakota State Univ, Dept Plant Sci, Dept Biol & Microbiol, Brookings, SD 57007 USA. EM shinyi.marzano@sdstate.edu; leslie.domier@ars.usda.gov FU National Sclerotinia Initiative; United States Department of Agriculture FX This work, including the efforts of Shin-Yi Lee Marzano, Berlin Nelson, Glen Hartman, Darin M. Eastburn, and Leslie L. Domier, was funded by National Sclerotinia Initiative. This work, including the efforts of Glen Hartman and Leslie L. Domier, was funded by United States Department of Agriculture. NR 99 TC 4 Z9 4 U1 16 U2 17 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD AUG PY 2016 VL 90 IS 15 BP 6846 EP 6863 DI 10.1128/JVI.00357-16 PG 18 WC Virology SC Virology GA DU6EF UT WOS:000382306100019 PM 27194764 ER PT J AU Plowright, RK Peel, AJ Streicker, DG Gilbert, AT McCallum, H Wood, J Baker, ML Restif, O AF Plowright, Raina K. Peel, Alison J. Streicker, Daniel G. Gilbert, Amy T. McCallum, Hamish Wood, James Baker, Michelle L. Restif, Olivier TI Transmission or Within-Host Dynamics Driving Pulses of Zoonotic Viruses in Reservoir-Host Populations SO PLOS NEGLECTED TROPICAL DISEASES LA English DT Review ID BATS ROUSETTUS-AEGYPTIACUS; HENDRA VIRUS; NIPAH VIRUS; FRUIT BATS; EXPERIMENTAL INOCULATION; PTEROPUS-POLIOCEPHALUS; IMMUNE-RESPONSES; EMERGING VIRUSES; RABIES VIRUS; EBOLA-VIRUS AB Progress in combatting zoonoses that emerge from wildlife is often constrained by limited knowledge of the biology of pathogens within reservoir hosts. We focus on the host-pathogen dynamics of four emerging viruses associated with bats: Hendra, Nipah, Ebola, and Marburg viruses. Spillover of bat infections to humans and domestic animals often coincides with pulses of viral excretion within bat populations, but the mechanisms driving such pulses are unclear. Three hypotheses dominate current research on these emerging bat infections. First, pulses of viral excretion could reflect seasonal epidemic cycles driven by natural variations in population densities and contact rates among hosts. If lifelong immunity follows recovery, viruses may disappear locally but persist globally through migration; in either case, new outbreaks occur once births replenish the susceptible pool. Second, epidemic cycles could be the result of waning immunity within bats, allowing local circulation of viruses through oscillating herd immunity. Third, pulses could be generated by episodic shedding from persistently infected bats through a combination of physiological and ecological factors. The three scenarios can yield similar patterns in epidemiological surveys, but strategies to predict or manage spillover risk resulting from each scenario will be different. We outline an agenda for research on viruses emerging from bats that would allow for differentiation among the scenarios and inform development of evidence-based interventions to limit threats to human and animal health. These concepts and methods are applicable to a wide range of pathogens that affect humans, domestic animals, and wildlife. C1 [Plowright, Raina K.] Montana State Univ, Dept Microbiol & Immunol, Bozeman, MT 59717 USA. [Plowright, Raina K.] Penn State Univ, Ctr Infect Dis Dynam, State Coll, PA USA. [Peel, Alison J.] Griffith Univ, Environm Futures Res Inst, Brisbane, Qld, Australia. [Streicker, Daniel G.] Univ Glasgow, Inst Biodivers Anim Hlth & Comparat Med, Glasgow, Lanark, Scotland. [Streicker, Daniel G.] MRC Univ Glasgow, Ctr Virus Res, Glasgow, Lanark, Scotland. [Gilbert, Amy T.] WS, USDA, APHIS, Natl Wildlife Res Ctr, Ft Collins, CO USA. [McCallum, Hamish] Griffith Univ, Griffith Sch Environm, Brisbane, Qld 4111, Australia. [Wood, James; Restif, Olivier] Univ Cambridge, Dis Dynam Unit, Dept Vet Med, Cambridge, England. [Baker, Michelle L.] CSIRO, Hlth & Biosecur Business Unit, Australian Anim Hlth Lab, Geelong, Vic, Australia. RP Plowright, RK (reprint author), Montana State Univ, Dept Microbiol & Immunol, Bozeman, MT 59717 USA.; Plowright, RK (reprint author), Penn State Univ, Ctr Infect Dis Dynam, State Coll, PA USA. EM raina.plowright@montana.edu RI McCallum, Hamish/E-1638-2013; OI McCallum, Hamish/0000-0002-3493-0412; Streicker, Daniel/0000-0001-7475-2705 FU Commonwealth of Australia; State of New South Wales; State of Queensland under the National Hendra Virus Research Program; Rural Industries Research and Development Corporation (RIRDC); Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate (United States of America Department of Homeland Security); Fogarty International Center (National Institutes of Health); National Institutes of Health [P20GM103474, P30GM110732]; Montana University System Research Initiative [51040-MUSRI2015-03]; University Research Fellowship from the Royal Society [UF120164]; RIRDC; Queensland Government Accelerate Fellowship grant; Sir Henry Dale Fellowship - Wellcome Trust [102507/Z/13/Z]; Sir Henry Dale Fellowship - Royal Society [102507/Z/13/Z]; Alborada Trust; European Union [278976]; Australian Research Council [FT110100234] FX This work was supported by the Commonwealth of Australia, the State of New South Wales, and the State of Queensland under the National Hendra Virus Research Program, awarded through the Rural Industries Research and Development Corporation (RIRDC); the Research and Policy for Infectious Disease Dynamics (RAPIDD) program of the Science and Technology Directorate (United States of America Department of Homeland Security); and the Fogarty International Center (National Institutes of Health). RKP was supported by National Institutes of Health IDeA Program grants P20GM103474 and P30GM110732, P. Thye, and Montana University System Research Initiative 51040-MUSRI2015-03. OR was supported by a University Research Fellowship from the Royal Society (grant UF120164). AJP was supported by RIRDC and a Queensland Government Accelerate Fellowship grant. DGS was supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (grant number 102507/Z/13/Z). JW is supported by the Alborada Trust and the European Union FP7 project ANTIGONE (contract number 278976). MLB was supported by an Australian Research Council Future Fellowship (FT110100234). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 81 TC 1 Z9 2 U1 14 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1935-2735 J9 PLOS NEGLECT TROP D JI Plos Neglect. Trop. Dis. PD AUG PY 2016 VL 10 IS 8 AR e0004796 DI 10.1371/journal.pntd.0004796 PG 21 WC Infectious Diseases; Parasitology; Tropical Medicine SC Infectious Diseases; Parasitology; Tropical Medicine GA DU7JR UT WOS:000382390800005 PM 27489944 ER PT J AU Qiao, F Luo, LL Peng, H Luo, SJ Huang, WK Cui, JK Li, X Kong, LG Jiang, DH Chitwood, DJ Peng, DL AF Qiao, Fen Luo, Lilian Peng, Huan Luo, Shujie Huang, Wenkun Cui, Jiangkuan Li, Xin Kong, Lingan Jiang, Daohong Chitwood, David J. Peng, Deliang TI Characterization of Three Novel Fatty Acid- and Retinoid-Binding Protein Genes (Ha-far-1, Ha-far-2 and Hf-far-1) from the Cereal Cyst Nematodes Heterodera avenae and H. filipjevi SO PLOS ONE LA English DT Article ID CAENORHABDITIS-ELEGANS; ONCHOCERCA-VOLVULUS; FILARIAL NEMATODES; FAR PROTEINS; IDENTIFICATION; INFECTION; ROOTS; CHINA; EXPRESSION; PATTERN AB Heterodera avenae and H. filipjevi are major parasites of wheat, reducing production worldwide. Both are sedentary endoparasitic nematodes, and their development and parasitism depend strongly on nutrients obtained from hosts. Secreted fatty acid-and retinol-binding (FAR) proteins are nematode-specific lipid carrier proteins used for nutrient acquisition as well as suppression of plant defenses. In this study, we obtained three novel FAR genes Ha-far-1 (KU877266), Ha-far-2 (KU877267), Hf-far-1 (KU877268). Ha-far-1 and Ha-far-2 were cloned from H. avenae, encoding proteins of 191 and 280 amino acids with molecular masses about 17 and 30 kDa, respectively and sequence identity of 28%. Protein Blast in NCBI revealed that Ha-FAR-1 sequence is 78% similar to the Gp-FAR-1 protein from Globodera pallida, while Ha-FAR-2 is 30% similar to Rs-FAR-1 from Radopholus similis. Only one FAR protein Hf-FAR-1was identified in H. filipjevi; it had 96% sequence identity to HaFAR-1. The three proteins are alpha-helix-rich and contain the conserved domain of GpFAR-1, but Ha-FAR-2 had a remarkable peptide at the C-terminus which was random-coilrich. Both Ha-FAR-1 and Hf-FAR-1 had casein kinase II phosphorylation sites, while HaFAR-2 had predicted N-glycosylation sites. Phylogenetic analysis showed that the three proteins clustered together, though Ha-FAR-1 and Hf-FAR-1 adjoined each other in a plantparasitic nematode branch, but Ha-FAR-2 was distinct from the other proteins in the group. Fluorescence-based ligand binding analysis showed the three FAR proteins bound to a fluorescent fatty acid derivative and retinol and with dissociation constants similar to FARs from other species, though Ha-FAR-2 binding ability was weaker than that of the two others. In situ hybridization detected mRNAs of Ha-far-1 and Ha-far-2 in the hypodermis. The qRTPCR results showed that the Ha-far-1and Ha-far-2 were expressed in all developmental C1 [Qiao, Fen; Luo, Lilian; Peng, Huan; Luo, Shujie; Huang, Wenkun; Cui, Jiangkuan; Li, Xin; Kong, Lingan; Peng, Deliang] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Insect Pests & Plant Dis, Beijing 100193, Peoples R China. [Luo, Lilian; Jiang, Daohong] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei Province, Peoples R China. [Chitwood, David J.] USDA ARS, Nematol Lab, Bldg 011A,BARC West, Beltsville, MD 20705 USA. RP Peng, DL (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Insect Pests & Plant Dis, Beijing 100193, Peoples R China. EM dlpeng@ippcaas.cn FU National Natural Science Foundation of China [31571988]; National Key Basic Research Program of China (973 Program) [2013CB127502]; Special Fund for Agro-scientific Research in the Public Interest [201503114] FX This research work was supported by the National Natural Science Foundation of China (31571988), the National Key Basic Research Program of China (973 Program, 2013CB127502) and the Special Fund for Agro-scientific Research in the Public Interest (No. 201503114). NR 46 TC 2 Z9 2 U1 10 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 1 PY 2016 VL 11 IS 8 AR e0160003 DI 10.1371/journal.pone.0160003 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS9NO UT WOS:000381110300021 PM 27479008 ER PT J AU Zhao, CH Lin, YH Pan, YB Fu, HY Chen, RK Grisham, MP Li, Y Gao, SJ AF Zhao, Chun-Hui Lin, Yi-Hua Pan, Yong-Bao Fu, Hua-Ying Chen, Ru-Kai Grisham, Michael Paul Li, Yu Gao, San-Ji TI Population structure and selection pressure analysis among Sugarcane yellow leaf virus isolates based on P0 and P1 sequences SO TROPICAL PLANT PATHOLOGY LA English DT Article DE Sugarcane yellow leaf virus; Gene flow; Genetic variability; Population structure; Selection pressure ID DEPENDENT RNA-POLYMERASE; DNA POLYMORPHISM; MOLECULAR CHARACTERIZATION; GENETIC DIFFERENTIATION; GENOME CHARACTERIZATION; PHYLOGENETIC ANALYSIS; SILENCING SUPPRESSOR; EVOLUTIONARY HISTORY; MOTTLE-VIRUS; RECOMBINATION AB Yellow leaf of sugarcane is caused by Sugarcane yellow leaf virus (SCYLV) and has been a threat to global sugar industry. Understanding the diversity and genetic structure of plant virus populations may help to develop strategies to control virus-induced diseases. In this study, demography, genetic differentiation and selection pressure were assessed based on 57 nucleotide sequences of the P0 (567 nt) and 54 nucleotide sequences of the P1 (1413 nt) genes of SCYLV isolates. Phylogenetic analysis revealed three phylogeographical groups: African (Clade I), Chinese/American (Clade II), and Asian (Clade III). Genetic distance values between groups (0.034-0.250) were higher than within-group values (0.011-0.033). The African group was in a state of expansion, with lower nucleotide sequence diversity. Except for those isolates within the Asian group, SCYLV showed infrequent gene flow within or between phylogenetic groups. Analysis of selection pressure suggested that these two genes have been subjected to negative (purifying) selection. However, some sites, especially in the P0 coding region of the Chinese/American group, have undergone positive selection. Overall, aside from recombination and founder effects, gene flow and purifying selection are two important factors driving SCYLV evolution. C1 [Zhao, Chun-Hui; Fu, Hua-Ying; Chen, Ru-Kai; Gao, San-Ji] Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou 350002, Fujian, Peoples R China. [Lin, Yi-Hua] Fujian Inst Trop Crops, Zhangzhou 363000, Fujian, Peoples R China. [Pan, Yong-Bao; Grisham, Michael Paul] ARS, Sugarcane Res Unit, Houma, USDA, Louisiana, LA 70360 USA. [Li, Yu] Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Fujian, Peoples R China. RP Gao, SJ (reprint author), Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou 350002, Fujian, Peoples R China.; Li, Y (reprint author), Fujian Agr & Forestry Univ, Coll Crop Sci, Fuzhou 350002, Fujian, Peoples R China. EM YL0934@163.com; gaosanji@yahoo.com FU National Natural Science Foundation of China [31170345]; Earmarked Fund from the China Agriculture Research System [CARS-20-2-4]; Major Science and Technology Project of Fujian Province in China [2015NZ0002-2] FX This research was supported by a grant from the National Natural Science Foundation of China (31170345), an Earmarked Fund from the China Agriculture Research System (CARS-20-2-4), and the Major Science and Technology Project of Fujian Province (2015NZ0002-2) in China. NR 49 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1983-2052 J9 TROP PLANT PATHOL JI Trop. Plant Pathol. PD AUG PY 2016 VL 41 IS 4 BP 237 EP 245 DI 10.1007/s40858-016-0096-2 PG 9 WC Plant Sciences SC Plant Sciences GA DU7BL UT WOS:000382368200004 ER PT J AU Li, MC Wu, QL Song, KL Cheng, HN Suzuki, S Lei, TZ AF Li, Mei-Chun Wu, Qinglin Song, Kunlin Cheng, H. N. Suzuki, Shigehiko Lei, Tingzhou TI Chitin Nanofibers as Reinforcing and Antimicrobial Agents in Carboxymethyl Cellulose Films: Influence of Partial Deacetylation SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Carboxymethyl cellulose; Chitin nanofibers; Deacetylation; Interfacial interaction; Mechanical properties; Antimicrobial activity ID ALPHA-CHITIN; CRAB SHELLS; SILVER NANOPARTICLES; RENEWABLE RESOURCES; CHITOSAN NANOFIBERS; POLYMER BLENDS; UNIFORM WIDTH; 10-20 NM; NANOCRYSTALS; NANOCOMPOSITES AB The development of edible, environmentally friendly, mechanically strong and antimicrobial biopolymer films for active food packaging has gained considerable interest in recent years. The present work deals with the extraction and deacetylation of chitin nanofibers (ChNFs) from crab shells and their utilization as reinforcing and antimicrobial agents in carboxymethyl cellulose (CMC) films. ChNFs were successfully isolated from the speckled swimming crab shells for the first time through the multistep procedures involving deproteinization, demineralization, depigmentation and mechanical disintegration. Afterward, the partially deacetylated ChNFs (dChNFs) were obtained through alkali treatment. It was found that the partial deacetylation led to the exposure of more amino groups on the surface of dChNFs and thus remarkably improved their dispersion state in an aqueous solution. The ChNF/CMC and dChNF/CMC films comprising up to 10 wt % nanofibers were prepared through the solution casting method, and their performance was evaluated and contrasted in terms of mechanical properties and antimicrobial activities. The results showed that the dChNF/CMC films exhibited superior mechanical and antimicrobial performance over ChNF/CMC films at any loadings, demonstrating the importance of ChNF surface chemistry in the development of high-performance ChNF/CMC films for antimicrobial food packaging application. C1 [Li, Mei-Chun; Wu, Qinglin; Song, Kunlin] Louisiana State Univ, AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Cheng, H. N.] USDA ARS, Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Suzuki, Shigehiko] Shizuoka Univ, Dept Environm & Forest Resources Sci, Fac Agr, Shizuoka 4228529, Japan. [Lei, Tingzhou] Henan Key Lab Biomass Energy, Zhengzhou 450008, Henan, Peoples R China. RP Wu, QL (reprint author), Louisiana State Univ, AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA.; Lei, TZ (reprint author), Henan Key Lab Biomass Energy, Zhengzhou 450008, Henan, Peoples R China. EM wuqing@lsu.edu; leitingzhou@163.com RI Song, Kunlin/B-2285-2017; Li, Meichun/F-7815-2015 OI Song, Kunlin/0000-0003-2141-1020; Li, Meichun/0000-0001-9381-0093 FU Louisiana Board of Regents [LEQSF-EPS (2014)-OPT-IN-37, LEQSF(2015-17)-RD-B-01]; USDA National Institute of Food and Agriculture McIntire Stennis project [1000017] FX This collaborative study was carried out with support from the Louisiana Board of Regents [LEQSF-EPS (2014)-OPT-IN-37, LEQSF(2015-17)-RD-B-01], and the USDA National Institute of Food and Agriculture McIntire Stennis project [1000017]. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 68 TC 2 Z9 2 U1 15 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD AUG PY 2016 VL 4 IS 8 BP 4385 EP 4395 DI 10.1021/acssuschemeng.6b00981 PG 11 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA DS6CR UT WOS:000380869800038 ER PT J AU Blanco-Rojo, R Delgado-Lista, J Lee, YC Lai, CQ Perez-Martinez, P Rangel-Zuniga, O Smith, CE Hidalgo, B Alcala-Diaz, JF Gomez-Delgado, F Parnell, LD Arnett, DK Tucker, KL Lopez-Miranda, J Ordovas, JM AF Blanco-Rojo, Ruth Delgado-Lista, Javier Lee, Yu-Chi Lai, Chao-Qiang Perez-Martinez, Pablo Rangel-Zuniga, Oriol Smith, Caren E. Hidalgo, Bertha Alcala-Diaz, Juan F. Gomez-Delgado, Francisco Parnell, Laurence D. Arnett, Donna K. Tucker, Katherine L. Lopez-Miranda, Jose Ordovas, Jose M. TI Interaction of an S100A9 gene variant with saturated fat and carbohydrates to modulate insulin resistance in 3 populations of different ancestries SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE gene-diet interaction; insulin resistance; SFA:carbohydrate ratio; S100A9 gene; type 2 diabetes ID FOOD-FREQUENCY QUESTIONNAIRE; GENOME-WIDE ASSOCIATION; BODY-MASS INDEX; METABOLIC SYNDROME; CARDIOVASCULAR-DISEASE; PHYSICAL-ACTIVITY; GLYCEMIC TRAITS; CLINICAL-TRIAL; DIET NETWORK; PUERTO-RICAN AB Background: S100 calcium-binding protein A9 (S100A9) has previously been identified as a type 2 diabetes (T2D) gene. However, this finding requires independent validation and more in-depth analyses in other populations and ancestries. Objectives: We aimed to replicate the associations between an S100A9 variant and insulin resistance and T2D and to initiate an investigation of potential interactions with the habitual diet in several independent populations. Design: We investigated the association of the S100A9 variant rs3014866 with insulin resistance and T2D risk and its interactions with diet in 3 diverse populations as follows: the CORDIOPREV (Coronary Diet Intervention with Olive Oil and Cardiovascular Prevention; n = 711), which consisted of Spanish white adults; the GOLDN (Genetics of Lipids Lowering Drugs and Diet Network; n = 818), which involved North American non-Hispanic white adults; and Hispanic adults who participated in the BPRHS (Boston Puerto Rican Health Study; n = 1155). Results: Meta-analysis indicated that T carriers presented a lower risk of T2D than CC carriers (pooled OR: 0.714; 95% CI: 0.584, 0.845; P = 0.002). In all 3 populations (CORDIOPREV, GOLDN, and BPRHS), we showed a significant interaction between the rs3014866 single nucleotide polymorphism and dietary SFA: carbohydrate ratio intake for the homeostasis model assessment of insulin resistance (HOMA-IR) (P = 0.028, P = 0.017, and P = 0.026, respectively). CC carriers had a significantly higher HOMA-IR only when SFA: carbohydrate intake was high (P = 0.045 for the CORDIOPREV, P = 0.033 for the GOLDN, and P = 0.046 for the BPRHS) but not when SFA: carbohydrate ratio intake was low. Conclusions: The minor allele (T) of the S100A9 variant rs3014866 is associated with lower T2D risk in 3 populations of different ancestries. Note that individuals with the high-risk CC genotype may be more likely to benefit from a low SFA: carbohydrate ratio intake to improve insulin resistance as evaluated with the use of the HOMA-IR. C1 [Blanco-Rojo, Ruth; Delgado-Lista, Javier; Perez-Martinez, Pablo; Rangel-Zuniga, Oriol; Alcala-Diaz, Juan F.; Gomez-Delgado, Francisco; Lopez-Miranda, Jose] Univ Cordoba, Maimonides Inst Biomed Res Cordoba, Reina Sofia Univ Hosp, Lipids & Atherosclerosis Unit, Cordoba, Spain. [Blanco-Rojo, Ruth; Delgado-Lista, Javier; Perez-Martinez, Pablo; Rangel-Zuniga, Oriol; Alcala-Diaz, Juan F.; Gomez-Delgado, Francisco; Lopez-Miranda, Jose] Inst Salud Carlos III, Ctr Invest Biomed Red CIBER Fisiopatol Obesidad &, Madrid, Spain. [Blanco-Rojo, Ruth; Lee, Yu-Chi; Smith, Caren E.; Ordovas, Jose M.] Tufts Univ, USDA, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Lai, Chao-Qiang; Parnell, Laurence D.] Tufts Univ, USDA, Jean Mayer USDA Human Nutr Res Ctr Aging, ARS, Boston, MA 02111 USA. [Hidalgo, Bertha; Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Tucker, Katherine L.] Univ Massachusetts, Dept Clin Lab & Nutr Sci, Lowell, MA USA. [Ordovas, Jose M.] Spanish Natl Ctr Cardiovasc Res CNIC, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Madrid Inst Adv Studies IMDEA, Food Inst, Madrid, Spain. RP Lopez-Miranda, J (reprint author), Univ Cordoba, Maimonides Inst Biomed Res Cordoba, Reina Sofia Univ Hosp, Lipids & Atherosclerosis Unit, Cordoba, Spain.; Lopez-Miranda, J (reprint author), Inst Salud Carlos III, Ctr Invest Biomed Red CIBER Fisiopatol Obesidad &, Madrid, Spain.; Ordovas, JM (reprint author), Tufts Univ, USDA, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA.; Ordovas, JM (reprint author), Spanish Natl Ctr Cardiovasc Res CNIC, Dept Epidemiol, Madrid, Spain.; Ordovas, JM (reprint author), Madrid Inst Adv Studies IMDEA, Food Inst, Madrid, Spain. EM jose.ordovas@tufts.edu; jlopezmir@uco.es OI Gomez Delgado, Francisco/0000-0002-0216-2084 FU Instituto de Salud Carlos III (ISCIII); National Heart, Lung, and Blood Institute, NIH [K08HL112845]; Ministerio de Economia y Competitividad, Spain [AGL2012/39615]; Ministerio de Ciencia e Innovacion, Spain [PIE14/00005, PI13/00023]; European Commission (NUTRITECH European Integrated Project) [289511]; Fundacion Patrimonio Comunal Olivarero; Centro Tecnologico del Olivar y del Aceite; Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud; Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa); Diputaciones de Jaen y Cordoba; Ministerio de Medio Ambiente; Medio Rural y Marino; Spanish Government; Fondo Europeo de Desarrollo Regional; National Heart, Lung, and Blood Institute (NHLBI) [U01HL072524]; NHLBI [R01 HL091357, HL54776, HL078885]; National Heart, Lung, and Blood Institute (NHLBI) from the USDA, Agriculture Research Service [53-K06-5-10, 58-1950-9-001]; NIH [P01 AG023394, P50 HL105185] FX Supported by an Instituto de Salud Carlos III (ISCIII) postdoctoral research contract [Sara Borrell (Spain); to RB-R]. CES is supported by grant K08HL112845 from the National Heart, Lung, and Blood Institute, NIH. The CORDIOPREV (Coronary Diet Intervention with Olive Oil and Cardiovascular Prevention) study was supported by the Ministerio de Economia y Competitividad, Spain (grant AGL2012/39615; to JL-M) and the Ministerio de Ciencia e Innovacion, Spain [grants PIE14/00005 (to JL-M) and PI13/00023 (to JD-L)] and was also partly supported by a research grant from the European Commission (NUTRITECH European Integrated Project-289511). The CORDIOPREV study was also supported by the Fundacion Patrimonio Comunal Olivarero. Additional funding was received from Centro Tecnologico del Olivar y del Aceite, Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud, Junta de Andalucia (Consejeria de Salud, Consejeria de Agricultura y Pesca, Consejeria de Innovacion, Ciencia y Empresa), the Diputaciones de Jaen y Cordoba, the Centro de Excelencia en Investigacion sobre Aceite de Oliva y Salud and Ministerio de Medio Ambiente, the Medio Rural y Marino, and the Spanish Government. The study was also cofinanced by the Fondo Europeo de Desarrollo Regional. The CIBER Fisiopatologia Obesidad y Nutricion is an initiative of the ISCIII, Madrid, Spain. The Genetics of Lipids Lowering Drugs and Diet Network study was supported by the National Heart, Lung, and Blood Institute (NHLBI) [grants U01HL072524 (Genetic and Environmental Determinants of Triglycerides), NHLBI R01 HL091357 (Genomewide Association Study of Lipid Response to Fenofibrate and Dietary Fat), HL54776, and HL078885 and by contracts 53-K06-5-10 and 58-1950-9-001 from the USDA, Agriculture Research Service]. The Boston Puerto Rican Health Study was supported by the NIH (grants P01 AG023394 and P50 HL105185). NR 52 TC 0 Z9 0 U1 5 U2 7 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD AUG 1 PY 2016 VL 104 IS 2 BP 508 EP 517 DI 10.3945/ajcn.116.130898 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DU0CZ UT WOS:000381870200032 PM 27440084 ER PT J AU Jarugula, S Charlesworth, SR Qu, F Stewart, LR AF Jarugula, Sridhar Charlesworth, Steven R. Qu, Feng Stewart, Lucy R. TI Soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity SO ARCHIVES OF VIROLOGY LA English DT Article DE SBWMV; Furovirus; Infectious clone; Gene silencing ID SILENCING VECTOR; RNA2; REPLICATION; PROTEIN; BARLEY; STRAIN; P19 AB A full-length infectious cDNA clone of soil-borne wheat mosaic virus (SBWMV; genus Furovirus; family Virgaviridae) was developed for agrobacterium delivery. The cloned virus can be agroinfiltrated to Nicotiana benthamiana for subsequent infection of wheat (Triticum aestivum, L.). The utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of RNA2. Virus-induced photobleaching was observed in N. benthamiana but not in wheat, despite the stability of the inserts. The SBWMV infectious clone can be used for further studies to investigate the biology of SBWMV through mutagenesis. C1 [Jarugula, Sridhar; Charlesworth, Steven R.; Qu, Feng; Stewart, Lucy R.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Ctr Appl Plant Sci, Wooster, OH 44691 USA. [Jarugula, Sridhar; Qu, Feng; Stewart, Lucy R.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Stewart, Lucy R.] ARS, USDA, Corn Soybean & Wheat Qual Res Unit, OARDC, 1680 Madison Ave,023 Selby Hall, Wooster, OH 44691 USA. [Charlesworth, Steven R.] Queensland Univ Technol, Ctr Trop Crops & Biocommod, Brisbane, Qld 4000, Australia. RP Stewart, LR (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Ctr Appl Plant Sci, Wooster, OH 44691 USA.; Stewart, LR (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA.; Stewart, LR (reprint author), ARS, USDA, Corn Soybean & Wheat Qual Res Unit, OARDC, 1680 Madison Ave,023 Selby Hall, Wooster, OH 44691 USA. EM lucy.stewart@ars.usda.gov FU Center for Applied Plant Sciences (CAPS), Ohio State University (OSU); United States Department of Agriculture-Agricultural Research Service (USDA-ARS) FX The project was supported by the Center for Applied Plant Sciences (CAPS), Ohio State University (OSU), and the United States Department of Agriculture-Agricultural Research Service (USDA-ARS). We thank Kristen Willie (USDA-ARS) and Benjamin Stromberg (College of Wooster) for technical assistance, and the staff at the OSU Molecular and Cellular Imaging Center (MCIC) for microscopy support. NR 23 TC 0 Z9 0 U1 7 U2 7 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD AUG PY 2016 VL 161 IS 8 BP 2291 EP 2297 DI 10.1007/s00705-016-2863-2 PG 7 WC Virology SC Virology GA DR8FZ UT WOS:000380135100026 PM 27236459 ER PT J AU Fillmer, K Adkins, S Pongam, P D'Elia, T AF Fillmer, Kornelia Adkins, Scott Pongam, Patchara D'Elia, Tom TI The complete nucleotide sequence and genomic characterization of tropical soda apple mosaic virus SO ARCHIVES OF VIROLOGY LA English DT Article ID VIEWER AB We report the first complete genome sequence of tropical soda apple mosaic virus (TSAMV), a tobamovirus originally isolated from tropical soda apple (Solanum viarum) collected in Okeechobee, Florida. The complete genome of TSAMV is 6,350 nucleotides long and contains four open reading frames encoding the following proteins: i) 126-kDa methyltransferase/helicase (3354 nt), ii) 183-kDa polymerase (4839 nt), iii) movement protein (771 nt) and iv) coat protein (483 nt). The complete genome sequence of TSAMV shares 80.4 % nucleotide sequence identity with pepper mild mottle virus (PMMoV) and 71.2-74.2 % identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the deduced amino acid sequences of the 126-kDa and 183-kDa proteins and the complete genome sequence place TSAMV in a subcluster with PMMoV within the Solanaceae-infecting subgroup of tobamoviruses. C1 [Fillmer, Kornelia; Pongam, Patchara; D'Elia, Tom] Indian River State Coll, Dept Biol, 3209 Virginia Ave, Ft Pierce, FL 34981 USA. [Adkins, Scott] ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. RP D'Elia, T (reprint author), Indian River State Coll, Dept Biol, 3209 Virginia Ave, Ft Pierce, FL 34981 USA.; Adkins, S (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM scott.adkins@ars.usda.gov; tdelia@irsc.edu FU Banack Family Partnership Endowed Teaching Chairs in Agriculture FX We thank Carrie Vanderspool, Megan Carroll and Yaowapa Tantiwanich for their excellent technical assistance. Financial support was provided in part by The Banack Family Partnership Endowed Teaching Chairs in Agriculture to P.P. and T.D. NR 13 TC 0 Z9 0 U1 3 U2 3 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD AUG PY 2016 VL 161 IS 8 BP 2317 EP 2320 DI 10.1007/s00705-016-2888-6 PG 4 WC Virology SC Virology GA DR8FZ UT WOS:000380135100030 PM 27169599 ER PT J AU Afonso, CL Amarasinghe, GK Banyai, K Bao, YM Basler, CF Bavari, S Bejerman, N Blasdell, KR Briand, FX Briese, T Bukreyev, A Calisher, CH Chandran, K Cheng, JS Clawson, AN Collins, PL Dietzgen, RG Dolnik, O Domier, LL Durrwald, R Dye, JM Easton, AJ Ebihara, H Farkas, SL Freitas-Astua, J Formenty, P Fouchier, RAM Fu, YP Ghedin, E Goodin, MM Hewson, R Horie, M Hyndman, TH Jiang, DH Kitajima, EW Kobinger, GP Kondo, H Kurath, G Lamb, RA Lenardon, S Leroy, EM Li, CX Lin, XD Liu, LJ Longdon, B Marton, S Maisner, A Muhlberger, E Netesov, SV Nowotny, N Patterson, JL Payne, SL Paweska, JT Randall, RE Rima, BK Rota, P Rubbenstroth, D Schwemmle, M Shi, M Smither, SJ Stenglein, MD Stone, DM Takada, A Terregino, C Tesh, RB Tian, JH Tomonaga, K Tordo, N Towner, JS Vasilakis, N Verbeek, M Volchkov, VE Wahl-Jensen, V Walsh, JA Walker, PJ Wang, D Wang, LF Wetzel, T Whitfield, AE Xie, JT Yuen, KY Zhang, YZ Kuhn, JH AF Afonso, Claudio L. Amarasinghe, Gaya K. Banyai, Krisztian Bao, Yiming Basler, Christopher F. Bavari, Sina Bejerman, Nicolas Blasdell, Kim R. Briand, Francois-Xavier Briese, Thomas Bukreyev, Alexander Calisher, Charles H. Chandran, Kartik Cheng, Jiasen Clawson, Anna N. Collins, Peter L. Dietzgen, Ralf G. Dolnik, Olga Domier, Leslie L. Duerrwald, Ralf Dye, John M. Easton, Andrew J. Ebihara, Hideki Farkas, Szilvia L. Freitas-Astua, Juliana Formenty, Pierre Fouchier, Ron A. M. Fu, Yanping Ghedin, Elodie Goodin, Michael M. Hewson, Roger Horie, Masayuki Hyndman, Timothy H. Jiang, Daohong Kitajima, Elliot W. Kobinger, Gary P. Kondo, Hideki Kurath, Gael Lamb, Robert A. Lenardon, Sergio Leroy, Eric M. Li, Ci-Xiu Lin, Xian-Dan Liu, Lijiang Longdon, Ben Marton, Szilvia Maisner, Andrea Muhlberger, Elke Netesov, Sergey V. Nowotny, Norbert Patterson, Jean L. Payne, Susan L. Paweska, Janusz T. Randall, Rick E. Rima, Bertus K. Rota, Paul Rubbenstroth, Dennis Schwemmle, Martin Shi, Mang Smither, Sophie J. Stenglein, Mark D. Stone, David M. Takada, Ayato Terregino, Calogero Tesh, Robert B. Tian, Jun-Hua Tomonaga, Keizo Tordo, Noel Towner, Jonathan S. Vasilakis, Nikos Verbeek, Martin Volchkov, Viktor E. Wahl-Jensen, Victoria Walsh, John A. Walker, Peter J. Wang, David Wang, Lin-Fa Wetzel, Thierry Whitfield, Anna E. Xie, Jiatao Yuen, Kwok-Yung Zhang, Yong-Zhen Kuhn, Jens H. TI Taxonomy of the order Mononegavirales: update 2016 SO ARCHIVES OF VIROLOGY LA English DT Article ID COMPLETE GENOME SEQUENCE; PARROT BORNAVIRUS 5; AVIAN PARAMYXOVIRUS; INTERNATIONAL COMMITTEE; FELINE MORBILLIVIRUS; RATIFICATION VOTE; DOMESTIC CATS; VIRUS; IDENTIFICATION; PROPOSALS AB In 2016, the order Mononegavirales was emended through the addition of two new families (Mymonaviridae and Sunviridae), the elevation of the paramyxoviral subfamily Pneumovirinae to family status (Pneumoviridae), the addition of five free-floating genera (Anphevirus, Arlivirus, Chengtivirus, Crustavirus, and Wastrivirus), and several other changes at the genus and species levels. This article presents the updated taxonomy of the order Mononegavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV). C1 [Afonso, Claudio L.] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30602 USA. [Amarasinghe, Gaya K.] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63130 USA. [Banyai, Krisztian; Farkas, Szilvia L.; Marton, Szilvia] Hungarian Acad Sci, Vet Med Res Inst, Agr Res Ctr, Budapest, Hungary. [Bao, Yiming] NIH, Informat Engn Branch, Natl Ctr Biotechnol Informat, Natl Lib Med, Bldg 10, Bethesda, MD 20892 USA. [Basler, Christopher F.] Georgia State Univ, Ctr Microbial Pathogenesis, Inst Biomed Sci, Atlanta, GA 30303 USA. [Bavari, Sina; Dye, John M.] United States Army Med Res Inst Infect Dis, Frederick, MD USA. [Bejerman, Nicolas; Lenardon, Sergio] Inst Nacl Tecnol Agr, Inst Patol Vegetal, Ctr Invest Agr, Cordoba, Argentina. [Bejerman, Nicolas] Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina. [Blasdell, Kim R.; Walker, Peter J.] CSIRO Hlth & Biosecur, Australian Anim Hlth Lab, Geelong, Vic, Australia. [Briand, Francois-Xavier] French Agcy Food Environm & Occupat Hlth & Safety, Avian & Rabbit Virol Immunol & Parasitol Unit, Ploufragan, France. [Briese, Thomas] Columbia Univ, Mailman Sch Publ Hlth, Ctr Infect & Immun, New York, NY 10027 USA. [Bukreyev, Alexander; Tesh, Robert B.] Univ Texas Med Branch, Dept Pathol, Galveston Natl Lab, Galveston, TX 77555 USA. [Bukreyev, Alexander; Tesh, Robert B.] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston Natl Lab, Galveston, TX 77555 USA. [Calisher, Charles H.] Colorado State Univ, Arthropod Borne & Infect Dis Lab, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. [Chandran, Kartik] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA. [Cheng, Jiasen; Fu, Yanping; Jiang, Daohong; Liu, Lijiang; Xie, Jiatao] Huazhong Agr Univ, State Key Lab Agr Microbiol, Prov Key Lab Plant Pathol Hubei Prov, Coll Plant Sci & Technol, Wuhan, Peoples R China. [Clawson, Anna N.; Kuhn, Jens H.] NIAID, Integrated Res Facil Ft Detrick IRF Frederick, Div Clin Res, NIH, B-8200 Res Plaza, Frederick, MD 21702 USA. [Collins, Peter L.] NIAID, Resp Viruses Sect, Infect Dis Lab, NIH, Bethesda, MD 21702 USA. [Dietzgen, Ralf G.] Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld, Australia. [Dolnik, Olga; Maisner, Andrea] Univ Marburg, Inst Virol, Marburg, Germany. [Domier, Leslie L.] Univ Illinois, Dept Crop Sci, Champaign, IL 61820 USA. [Duerrwald, Ralf] IDT Biol, Dessau Rosslau, Germany. [Easton, Andrew J.; Walsh, John A.] Univ Warwick, Sch Life Sci, Coventry, W Midlands, England. [Ebihara, Hideki] NIAID, Rocky Mt Labs, Integrated Res Facil, NIH, Hamilton, MT 59840 USA. [Freitas-Astua, Juliana] Embrapa Cassava & Fruits, Cruz Das Almas, BA, Brazil. [Formenty, Pierre] WHO, Geneva, Switzerland. [Fouchier, Ron A. M.] Erasmus Univ, Med Ctr, Dept Virosci, Postgrad Sch Mol Med, Rotterdam, Netherlands. [Ghedin, Elodie] NYU, Dept Biol, Ctr Genom & Syst Biol, New York, NY 10003 USA. [Goodin, Michael M.] Univ Kentucky, Plant Pathol, Lexington, KY 40506 USA. [Hewson, Roger] Publ Hlth England, Salisbury, Wilts, England. [Horie, Masayuki] Kagoshima Univ, Joint Fac Vet Med, Transboundary Anim Dis Res Ctr, Kagoshima, Japan. [Hyndman, Timothy H.] Murdoch Univ, Sch Vet & Life Sci, Murdoch, WA, Australia. [Kitajima, Elliot W.] Univ Sao Paulo, Nucleo Apoio Pesquisa Microscopia Eletr Aplicada, Escola Super Agr Luiz de Queiroz, Sao Paulo, Brazil. [Kobinger, Gary P.] Publ Hlth Agcy Canada, Special Pathogens Program, Natl Microbiol Lab, Winnipeg, MB, Canada. [Kondo, Hideki] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama, Japan. [Kurath, Gael] US Geol Survey, Western Fisheries Res Ctr, Seattle, WA 98115 USA. [Lamb, Robert A.] Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA. [Lamb, Robert A.] Northwestern Univ, Howard Hughes Med Inst, Evanston, IL 60208 USA. [Leroy, Eric M.] Ctr Int Rech Med Franceville, Inst Rech Dev, Franceville, Gabon. [Shi, Mang; Zhang, Yong-Zhen] Chinese Ctr Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China. [Li, Ci-Xiu] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China. [Lin, Xian-Dan] Wenzhou Ctr Dis Control & Prevent, Wenzhou, Peoples R China. [Longdon, Ben] Univ Cambridge, Dept Genet, Cambridge, England. [Muhlberger, Elke] Boston Univ, Sch Med, Dept Microbiol, Boston, MA 02118 USA. [Muhlberger, Elke] Boston Univ, Sch Med, Natl Emerging Infect Dis Lab, Boston, MA 02118 USA. [Netesov, Sergey V.] Novosibirsk State Univ, Novosibirsk, Novosibirsk Obl, Russia. [Nowotny, Norbert] Univ Vet Med, Inst Virol, Vienna, Austria. [Nowotny, Norbert] Mohammed Bin Rashid Univ Med & Hlth Sci, Dept Basic Med Sci, Coll Med, Dubai, U Arab Emirates. [Patterson, Jean L.] Texas Biomed Res Inst, Dept Virol & Immunol, San Antonio, TX 78227 USA. [Payne, Susan L.] Texas A&M Univ, Dept Vet Pathobiol, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Paweska, Janusz T.] Natl Inst Communicable Dis, Ctr Emerging & Zoonot Dis, Natl Hlth Lab Serv, Sandringham Johannesburg, Gauteng, South Africa. [Randall, Rick E.] Univ St Andrews, Biomed Sci Res Complex, St Andrews, Fife, Scotland. [Rima, Bertus K.] Queens Univ Belfast, Ctr Med Expt, Sch Med Dent & Biomed Sci, Belfast, Antrim, North Ireland. [Rota, Paul] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Atlanta, GA 30333 USA. [Rubbenstroth, Dennis; Schwemmle, Martin] Univ Freiburg, Inst Virol, Fac Med, Med Ctr, Freiburg, Germany. [Smither, Sophie J.] CBR Div, Dstl, Salisbury, Wilts, England. [Stenglein, Mark D.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. [Stone, David M.] Ctr Environm Fisheries & Aquaculture Sci, Weymouth, Dorset, England. [Takada, Ayato] Hokkaido Univ, Res Ctr Zoonosis Control, Div Global Epidemiol, Sapporo, Hokkaido, Japan. [Terregino, Calogero] OIE Collaborating Ctr Dis Human Anim Interface, Ist Zooprofilatt Sperimentale Venezie,FAO Referen, Dept Comparat Biomed Sci, Natl OIE Reference Lab Newcastle Dis & Avian Infl, Padua, Italy. [Tian, Jun-Hua] Wuhan Ctr Dis Control & Prevent, Wuhan, Peoples R China. [Tomonaga, Keizo] Kyoto Univ, Inst Virus Res, Kyoto, Japan. [Tordo, Noel] Inst Pasteur, Unite Strategies Antivirales, Paris, France. [Tordo, Noel] Inst Pasteur Guinee, Conakry, Guinea. [Towner, Jonathan S.] Ctr Dis Control & Prevent, Viral Special Pathogens Branch, Div High Consequence Pathogens Pathol, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Vasilakis, Nikos] Univ Texas Med Branch, Ctr Biodefense & Emerging Infect Dis, Dept Pathol, Galveston, TX 77555 USA. [Vasilakis, Nikos] Univ Texas Med Branch, Ctr Trop Dis, Inst Human Infect & Immun, Galveston, TX 77555 USA. [Verbeek, Martin] Wageningen Univ & Res, Wageningen, Netherlands. [Volchkov, Viktor E.] Univ Lyon 1, Mol Basis Viral Pathogen, CIRI, INSERM,U1111,CNRS,UMR5308,Ecole Normal Super Lyon, Lyon, France. [Wahl-Jensen, Victoria] Natl Biodef Anal & Countermeasures Ctr, Frederick, MD 21702 USA. [Wang, David] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA. [Wang, David] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA. [Wang, Lin-Fa] Biosecur Queensland, Dept Agr & Fisheries, Brisbane, Qld, Australia. [Wang, Lin-Fa] Duke NUS Grad Med Sch, Program Emerging Infect Dis, Singapore, Singapore. [Wetzel, Thierry] DLR Rheinpfalz, Inst Plant Protect, Neustadt, Germany. [Whitfield, Anna E.] Kansas State Univ, Plant Pathol, Manhattan, KS 66506 USA. [Yuen, Kwok-Yung] Univ Hong Kong, State Key Lab Emerging Infect Dis, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China. RP Kuhn, JH (reprint author), NIAID, Integrated Res Facil Ft Detrick IRF Frederick, Div Clin Res, NIH, B-8200 Res Plaza, Frederick, MD 21702 USA. EM kuhnjens@mail.nih.gov RI Netesov, Sergey/A-3751-2013; Fouchier, Ron/A-1911-2014; Verbeek, Martin/D-1445-2017; Stenglein, Mark/E-3541-2017; LEROY, Eric/I-4347-2016; OI Netesov, Sergey/0000-0002-7786-2464; Fouchier, Ron/0000-0001-8095-2869; Verbeek, Martin/0000-0002-8973-3803; Stenglein, Mark/0000-0002-0993-813X; LEROY, Eric/0000-0003-0022-0890; Freitas-Astua, Juliana/0000-0002-0506-6880; Blasdell, Kim/0000-0003-2121-0376; Banyai, Krisztian/0000-0002-6270-1772 FU Battelle Memorial Institute; US National Institute of Allergy and Infectious Diseases (NIAID) [HHSN272200700016I]; DHS ST [HSHQDC-07-C-00020]; National Institutes of Health (NIH) [HHSN272201000040I/HHSN27200004/D04]; NIH, National Library of Medicine FX This work was supported in part through Battelle Memorial Institute's prime contract with the US National Institute of Allergy and Infectious Diseases (NIAID) under Contract No. HHSN272200700016I. A subcontractor to Battelle Memorial Institute who performed this work is: J. H. K., an employee of Tunnell Government Services, Inc. This work was also funded in part under Contract No. HSHQDC-07-C-00020 awarded by DHS S&T for the management and operation of the National Biodefense Analysis and Countermeasures Center (NBACC), a Federally Funded Research and Development Center (V. W.-J.); and National Institutes of Health (NIH) contract HHSN272201000040I/HHSN27200004/D04 (N. V., R. B. T.). Y. B. was supported by the Intramural Research Program of the NIH, National Library of Medicine. NR 31 TC 30 Z9 30 U1 7 U2 9 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD AUG PY 2016 VL 161 IS 8 BP 2351 EP 2360 DI 10.1007/s00705-016-2880-1 PG 10 WC Virology SC Virology GA DR8FZ UT WOS:000380135100037 PM 27216929 ER PT J AU Lee, JH Lin, LG Xu, PW Saito, KJ Wei, Q Meadows, AG Bongmba, OYN Pradhan, G Zheng, H Xu, Y Sun, YX AF Lee, Jong Han Lin, Ligen Xu, Pingwen Saito, Kenji Wei, Qiong Meadows, Adelina G. Bongmba, Odelia Y. N. Pradhan, Geetali Zheng, Hui Xu, Yong Sun, Yuxiang TI Neuronal Deletion of Ghrelin Receptor Almost Completely Prevents Diet-Induced Obesity SO DIABETES LA English DT Article ID BROWN ADIPOSE-TISSUE; SPONTANEOUS LOCOMOTOR-ACTIVITY; HORMONE SECRETAGOGUE RECEPTOR; HYPOTHALAMIC NEURONS; DOPAMINE TRANSPORTER; ENERGY-EXPENDITURE; MESSENGER-RNA; MICE; THERMOGENESIS; EXPRESSION AB Ghrelin signaling has major effects on energy and glucose homeostasis, but it is unknown whether ghrelin's functions are centrally and/or peripherally mediated. The ghrelin receptor, growth hormone secretagogue receptor (GHS-R), is highly expressed in the brain and detectable in some peripheral tissues. To understand the roles of neuronal GHS-R, we generated a mouse line where Ghsr gene is deleted in all neurons using synapsin 1 (Syn1)-Cre driver. Our data showed that neuronal Ghsr deletion abolishes ghrelin-induced spontaneous food intake but has no effect on total energy intake. Remarkably, neuronal Ghsr deletion almost completely prevented diet-induced obesity (DIO) and significantly improved insulin sensitivity. The neuronal Ghsr-deleted mice also showed improved metabolic flexibility, indicative of better adaption to different fuels. In addition, gene expression analysis suggested that hypothalamus and/or midbrain might be the sites that mediate the effects of GHS-R in thermogenesis and physical activity, respectively. Collectively, our results indicate that neuronal GHS-R is a crucial regulator of energy metabolism and a key mediator of DIO. Neuronal Ghsr deletion protects against DIO by regulating energy expenditure, not by energy intake. These novel findings suggest that suppressing central ghrelin signaling may serve as a unique antiobesity strategy. C1 [Lee, Jong Han; Lin, Ligen; Xu, Pingwen; Saito, Kenji; Wei, Qiong; Meadows, Adelina G.; Bongmba, Odelia Y. N.; Pradhan, Geetali; Xu, Yong; Sun, Yuxiang] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lin, Ligen] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China. [Wei, Qiong] Southeast Univ, Zhongda Hosp, Div Endocrinol, Nanjing, Jiangsu, Peoples R China. [Zheng, Hui; Sun, Yuxiang] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Sun, Yuxiang] Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA. RP Sun, YX (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA.; Sun, YX (reprint author), Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA.; Sun, YX (reprint author), Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA.; Sun, YX (reprint author), Texas A&M Univ, Dept Nutr & Food Sci, College Stn, TX 77843 USA. EM yuxiangs@tamu.edu FU Macau Science and Technology Development Fund [FDCT 042/2013/A2]; University of Macau [MYRG2014-00020-ICMS-QRCM]; National Institutes of Health [P30DK56338, 1T32HD071839, R01DK093587, R01DK101379]; U.S. Department of Agriculture [CRIS 3092-5-001-059]; American Heart Association [12IRG9230004, 14GRNT18990019]; American Diabetes Association [1-15-BS-177] FX This study was partly supported by the Macau Science and Technology Development Fund (FDCT 042/2013/A2 to L.L.), the Research Fund of the University of Macau (MYRG2014-00020-ICMS-QRCM to L.L.), an American Heart Association postdoctoral fellowship (to P.X.), and the National Institutes of Health (P30DK56338, 1T32HD071839 [principal investigator Morey W. Haymond], R01DK093587 to Y.X., and R01DK101379 to Y.X.). This study was supported by the U.S. Department of Agriculture (CRIS 3092-5-001-059 to Y.S.), the American Heart Association (12IRG9230004 and 14GRNT18990019 to Y.S.), and the American Diabetes Association (1-15-BS-177 to Y.S.). NR 50 TC 2 Z9 2 U1 4 U2 6 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA SN 0012-1797 EI 1939-327X J9 DIABETES JI Diabetes PD AUG PY 2016 VL 65 IS 8 BP 2169 EP 2178 DI 10.2337/db15-1587 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DS4KQ UT WOS:000380750400013 PM 27207529 ER PT J AU Maron, JL Smith, AL Ortega, YK Pearson, DE Callaway, RM AF Maron, John L. Smith, Alyssa Laney Ortega, Yvette K. Pearson, Dean E. Callaway, Ragan M. TI Negative plant-soil feedbacks increase with plant abundance, and are unchanged by competition SO ECOLOGY LA English DT Article DE coexistence; interspecific competition; plant abundance; plant distribution; plant-soil feedbacks; species-specific effects ID COMMUNITY STRUCTURE; TROPICAL TREE; INTERSPECIFIC COMPETITION; DENSITY-DEPENDENCE; BORNE PATHOGENS; DIVERSITY; GRASSLAND; BIOTA; CONSEQUENCES; PRODUCTIVITY AB Plant-soil feedbacks and interspecific competition are ubiquitous interactions that strongly influence the performance of plants. Yet few studies have examined whether the strength of these interactions corresponds with the abundance of plant species in the field, or whether feedbacks and competition interact in ways that either ameliorate or exacerbate their effects in isolation. We sampled soil from two intermountain grassland communities where we also measured the relative abundance of plant species. In greenhouse experiments, we quantified the direction and magnitude of plant-soil feedbacks for 10 target species that spanned a range of abundances in the field. In soil from both sites, plant-soil feedbacks were mostly negative, with more abundant species suffering greater negative feedbacks than rare species. In contrast, the average response to competition for each species was unrelated with its abundance in the field. We also determined how competitive response varied among our target species when plants competed in live vs. sterile soil. Interspecific competition reduced plant size, but the strength of this negative effect was unchanged by plant-soil feedbacks. Finally, when plants competed interspecifically, we asked how conspecific-trained, heterospecific-trained, and sterile soil influenced the competitive responses of our target species and how this varied depending on whether target species were abundant or rare in the field. Here, we found that both abundant and rare species were not as harmed by competition when they grew in heterospecific-trained soil compared to when they grew in conspecific-cultured soil. Abundant species were also not as harmed by competition when growing in sterile vs. conspecific-trained soil, but this was not the case for rare species. Our results suggest that abundant plants accrue species-specific soil pathogens to a greater extent than rare species. Thus, negative feedbacks may be critical for preventing abundant species from becoming even more abundant than rare species. C1 [Maron, John L.; Smith, Alyssa Laney; Pearson, Dean E.; Callaway, Ragan M.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Smith, Alyssa Laney] Virginia Polytech Inst & State Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Ortega, Yvette K.; Pearson, Dean E.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. [Callaway, Ragan M.] Univ Montana, Inst Ecosyst, Missoula, MT 59812 USA. RP Maron, JL (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. EM john.maron@mso.umt.edu FU National Science Foundation [EPS-1101342 (INSTEP 3)] FX Thanks to Lauren Waller for help with quantifying species abundance in the field and Kim Ledger for her assistance in setting up our greenhouse experiments. K. Baer, P. Hahn, R. Hegstad, Y. Lekberg, and L. Larios provided valuable comments on the manuscript. We thank the National Science Foundation Experimental Program to Stimulate Competitive Research Track-1 EPS-1101342 (INSTEP 3) for support. NR 46 TC 1 Z9 1 U1 36 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD AUG PY 2016 VL 97 IS 8 BP 2055 EP 2063 DI 10.1002/ecy.1431 PG 9 WC Ecology SC Environmental Sciences & Ecology GA DS4KI UT WOS:000380749600017 PM 27859206 ER PT J AU Webster, JR Knoepp, JD Swank, WT Miniat, CF AF Webster, J. R. Knoepp, J. D. Swank, W. T. Miniat, C. F. TI Evidence for a Regime Shift in Nitrogen Export from a Forested Watershed SO ECOSYSTEMS LA English DT Article DE watershed; nitrogen; budget; regime shift; clearcutting; stream ID DISSOLVED ORGANIC-CARBON; LONG-TERM CHANGES; SOUTHERN APPALACHIANS; NITRATE EXPORT; NUTRIENT RETENTION; SEASONAL PATTERNS; ELEMENT CYCLES; WESTERN OREGON; UNITED-STATES; BLACK LOCUST AB In this study, we document a functional regime shift in stream inorganic nitrogen (N) processing indicated by a major change in N export from a forested watershed. Evidence from 36 years of data following experimental clearcut logging at Coweeta Hydrologic Laboratory, NC, suggests that forest disturbance in this area can cause elevation of dissolved inorganic N (DIN) loss lasting decades or perhaps longer. This elevation of N export was apparently caused by an initial pulse of organic matter input, reduced vegetation uptake, increased mineralization of soil organic N, and N fixation by black locust-associated bacteria following clearcut logging. In forested reference watersheds at Coweeta, maximum DIN concentration occurs in summer when base flow is low, but the clearcut watershed shifted to a pattern of maximum winter DIN concentration. The seasonal pattern of DIN concentration and export from reference watersheds can be explained by terrestrial and in-stream processes, but following clearcutting, elevated DIN availability saturated both terrestrial and in-stream uptake, and the N export regime became dominated by hydrologic transport. We suggest that the long-term elevation of stream DIN concentration and export along with the changes in seasonality of DIN export and the relationship between concentration and discharge represent a functional regime shift initiated by forest disturbance. C1 [Webster, J. R.] Virginia Polytech Inst & State Univ, Dept Biol Sci, 1405 Perry St,1016 Derring Hall,MC 0406, Blacksburg, VA 24061 USA. [Knoepp, J. D.; Swank, W. T.; Miniat, C. F.] US Forest Serv, Coweeta Hydrol Lab, Ctr Forest Watershed Res, Southern Res Stn,USDA, Otto, NC 28763 USA. RP Webster, JR (reprint author), Virginia Polytech Inst & State Univ, Dept Biol Sci, 1405 Perry St,1016 Derring Hall,MC 0406, Blacksburg, VA 24061 USA. EM jwebster@vt.edu FU USDA Forest Service; Southern Research Station; Coweeta Hydrologic Laboratory; National Science Foundation; NSF [DEB0823293] FX We thank Bobbie Niederlehner for help with the statistical analyses. Many of the ideas in this paper resulted from discussions with Drs. Rhett Jackson, Durrell Scott, Stephen Schoenholtz, Jeb Barrett, Kevin McGuire, Brian Strahm, Mary Beth Adams, Sheila Christopher, and Charley Kelly. We also appreciate the helpful comments from two anonymous reviewers. The WS 7 study was supported by the USDA Forest Service, Southern Research Station, Coweeta Hydrologic Laboratory, and by a series of grants from the National Science Foundation. This analysis of long-term data was supported by NSF grant DEB0823293 to the Coweeta LTER program at the University of Georgia and by Coweeta Hydrologic Laboratory. NR 125 TC 0 Z9 0 U1 10 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1432-9840 EI 1435-0629 J9 ECOSYSTEMS JI Ecosystems PD AUG PY 2016 VL 19 IS 5 BP 881 EP 895 DI 10.1007/s10021-016-9974-1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA DS3FU UT WOS:000380669300008 ER PT J AU Mogren, CL Rand, TA Fausti, SW Lundgren, JG AF Mogren, Christina L. Rand, Tatyana A. Fausti, Scott W. Lundgren, Jonathan G. TI The Effects of Crop Intensification on the Diversity of Native Pollinator Communities SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE agrobiont; Cropland Data Layer; landscape simplification; Melissodes ID SOWN WILDFLOWER STRIPS; LAND-USE; ECOSYSTEM SERVICES; AGRICULTURAL INTENSIFICATION; FIELD MARGINS; BEES; CONSERVATION; ABUNDANCE; SCALE; CONSEQUENCES AB Increases in agricultural conversion are leading to declines in native grasslands and natural resources critical for beneficial insects. However, little is known regarding how these changes affect pollinator diversity. Land use types were categorized within 300m and 3km radii of pollinator sampling locations in Brookings County, SD. Pollinator abundance and species richness were regressed on the proportion of the landscape dedicated to row crops, grass and pasture, forage crops, small grains, and aquatic habitats using variance components modeling. Row crops had a negative effect on bee abundance at 300 m, after fixed effects modeling accounted for outliers skewing this relationship. At 3 km, corn positively affected bee abundance and richness, while soybean acreage decreased species richness. The landscape matrix of outlying sites consisted of large monocultured areas with few alternative habitat types available, leading to inflated populations of Melissodes and Halictidae. Syrphids had a positive parabolic relationship between diversity and row crops, indicating potential for competitive exclusion from intermediate landscapes. Unlike other studies, landscape diversity within 300m was not found to significantly benefit pollinator diversity. Within especially agriculturally developed areas of the region, high abundances of pollinators suggest selection for a few dominant species. There was no effect of forage crops or aquatic habitats on pollinator diversity, indicating that less highly managed areas still represent degraded habitat within the landscape. Incorporating pollinator-friendly crops at the farm level throughout the region is likely to enhance pollinator diversity by lessening the negative effects of large monocultures. C1 [Mogren, Christina L.; Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. [Mogren, Christina L.] Louisiana State Univ, Dept Entomol, AgCtr, 404 Life Sci, Baton Rouge, LA 70803 USA. [Rand, Tatyana A.] USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. [Fausti, Scott W.] South Dakota State Univ, Dept Econ, Brookings, SD 57007 USA. [Lundgren, Jonathan G.] Ecdysis Fdn, 46958 188th St, Estelline, SD 57234 USA. RP Mogren, CL (reprint author), USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA.; Mogren, CL (reprint author), Louisiana State Univ, Dept Entomol, AgCtr, 404 Life Sci, Baton Rouge, LA 70803 USA. EM cmogren@gmail.com; tatyana.rand@ars.usda.gov; scott.fausti@sdstate.edu; jgl.entomology@gmail.com FU USDA-ARS; National Honey Board Grant FX We would like to thank Janet Fergen, Ryan Bell, Jacob Pecenka, Nicole Berg, and Marissa Layman for their help with field sampling and data collection. Mark Longfellow provided valuable statistical advice. Karen Wright (University of New Mexico) assisted with identification of Melissodes specimens, and Jason Gibbs (Michigan State University) assisted with identification of Lasioglossum specimens. This research was funded by the USDA-ARS and a 2014 National Honey Board Grant. Mention of any proprietary products does not constitute endorsement by the USDA-ARS. NR 54 TC 0 Z9 0 U1 43 U2 58 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 865 EP 872 DI 10.1093/ee/nvw066 PG 8 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600011 PM 27271948 ER PT J AU Bateman, C Sigut, M Skelton, J Smith, KE Hulcr, J AF Bateman, Craig Sigut, Martin Skelton, James Smith, Katherine E. Hulcr, Jiri TI Fungal Associates of the Xylosandrus compactus (Coleoptera: Curculionidae, Scolytinae) Are Spatially Segregated on the Insect Body SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Ambrosiella; Fusarium solani; black twig borer; mycangium; mutualism ID REDBAY AMBROSIA BEETLE; SP-NOV; PHYLOGENETIC DIVERSITY; BARK BEETLES; FUSARIUM; EVOLUTION; SYMBIOSIS; DISEASE; COMPLEX; SITES AB Studies of symbioses have traditionally focused on explaining one-to-one interactions between organisms. In reality, symbioses are often much more dynamic. They can involve many interacting members, and change depending on context. In studies of the ambrosia symbiosis-the mutualism between wood borer beetles and fungi-two variables have introduced uncertainty when explaining interactions: imprecise symbiont identification, and disregard for anatomical complexity of the insects. The black twig borer, Xylosandrus compactus Eichhoff, is a globally invasive ambrosia beetle that infests > 200 plant species. Despite many studies on this beetle, reports of its primary symbionts are conflicting. We sampled adult X. compactus and infested plant material in central Florida to characterize the fungal symbiont community using dilution series, beetle partitioning, and DNA-based identification. X. compactus was consistently associated with two fungal taxa, Fusarium spp. and Ambrosiella xylebori. Multivariate analyses revealed that A. xylebori was strongly associated with the beetle mycangium while Fusarium spp. were associated with the abdomen and external surfaces. The Fusarium spp. carried by X. compactus are not members of the Ambrosia Fusarium Clade, and are probably not mutualists. Fungal community composition of the mycangium was less variable than external body surfaces, thus providing a more consistent fungal inoculum. This is the first report of spatial partitioning as a mechanism for maintenance of a multimember ambrosia fungus community. Our results provide an explanation for discrepancies among previous reports, and suggest that conflicting results are not due to differences in symbiont communities, but due to inconsistent and incomplete sampling. C1 [Bateman, Craig; Hulcr, Jiri] Univ Florida, Inst Food & Agr Sci, Dept Entomol & Nematol, POB 110410, Gainesville, FL 32611 USA. [Sigut, Martin] Univ Ostrava, Fac Sci, Dept Biol & Ecol, Dvorakova 7, CZ-70103 Ostrava, Czech Republic. [Skelton, James; Smith, Katherine E.; Hulcr, Jiri] Univ Florida, Inst Food & Agr Sci, Sch Forest Resources & Conservat, POB 110410, Gainesville, FL 32611 USA. [Smith, Katherine E.] US Forest Serv, Southern Inst Forest Genet, USDA, Southern Res Stn, Saucier, MS 39574 USA. RP Hulcr, J (reprint author), Univ Florida, Inst Food & Agr Sci, Dept Entomol & Nematol, POB 110410, Gainesville, FL 32611 USA.; Hulcr, J (reprint author), Univ Florida, Inst Food & Agr Sci, Sch Forest Resources & Conservat, POB 110410, Gainesville, FL 32611 USA. EM batemanc@gmail.com; marton.sigut@gmail.com; skel-to3@gmail.com; smithk@ufl.edu; hulcr@ufl.edu FU United States Department of Agriculture (USDA) Forest Service (FS)-SRS [14-CA-11330130-032]; USDA-FS-FHP [12-CA-11420004-042]; USDA Farm Bill [14-8130-0377-CA]; National Science Foundation [DEB 1256968]; Ministry of Education, Youth and Sports of the Czech Republic (NextGenProject) [CZ.1.07/2.3./20.0303]; Grant Agency of University of Ostrava [SGS21/PrF/2013, SGS28/PrF/2014]; Institute of Environmental Technologies [CZ.1.05/2.1.00/03.0100]; Grant Agency of the Czech Republic [GA14-04258S] FX We thank Kerry O'Donnell for a review of an initial draft. This project was funded by the United States Department of Agriculture (USDA) Forest Service (FS)-SRS Coop agreement 14-CA-11330130-032, USDA-FS-FHP Coop agreement 12-CA-11420004-042, USDA Farm Bill agreement 14-8130-0377-CA, the National Science Foundation DEB 1256968, the Ministry of Education, Youth and Sports of the Czech Republic (NextGenProject) CZ.1.07/2.3./20.0303, the Grant Agency of University of Ostrava SGS21/PrF/2013 and SGS28/PrF/2014, the Institute of Environmental Technologies CZ.1.05/2.1.00/03.0100, and the Grant Agency of the Czech Republic GA14-04258S. NR 65 TC 0 Z9 0 U1 10 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 883 EP 890 DI 10.1093/ee/nvw070 PG 8 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600013 PM 27357160 ER PT J AU Zhang, YZ Hanula, JL Horn, S Jones, C Braman, SK Sun, JH AF Zhang, Yanzhuo Hanula, James L. Horn, Scott Jones, Cera Braman, S. Kristine Sun, Jianghua TI Fundamental Host Range of Leptoypha hospita (Hemiptera: Tingidae), a Potential Biological Control Agent of Chinese Privet SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Ligustrum sinense; Oleeae; Forestiera acuminata; Forestiera pubescens; host specificity testing ID ARGOPISTES-TSEKOONI COLEOPTERA; LIGUSTRUM-SINENSE; RIPARIAN FORESTS; LIFE-HISTORY; OLEACEAE; SPECIFICITY; ABUNDANCE; PLANTS; SHRUB; CLASSIFICATION AB Chinese privet, Ligustrum sinense Lour., is an invasive shrub within riparian areas of the southeastern United States. Biological control is considered the most suitable management option for Chinese privet. The potential host range of the lace bug, Leptoypha hospita Drake et Poor, was evaluated on the basis of adult feeding and oviposition, combined oviposition-nymphal development no-choice tests, nymphal development no-choice tests, multiple generation comparison on Forestiera pubescens Nutt. and L. sinense no-choice tests, and multiple-choice tests with 45 plant species in 13 families. No-choice tests showed that the host range of L. hospita was restricted to the tribe Oleeae. In adult feeding and oviposition no-choice tests, the bug fed and oviposited significantly more on Chinese privet than all other test plant species except for three native Forestiera spp., two nonnative Syringa spp., and another exotic Ligustrum sp. Among those, only F. pubescens supported complete development in numbers comparable to Chinese privet. However, when reared for multiple generations lace bugs reared on F. pubescens were smaller and had lower fecundity than those reared on L. sinense, suggesting F. pubescens is not an optimal host. In multiple-choice tests, L. hospita displayed a strong preference for feeding and ovipositing on Chinese privet over other test plant species, with the exception of the closely related nonnative Syringa spp. and its congenic species Ligustrum vulgare. The results of this study suggest that the risk to nontarget plant species in North America is minimal, and L. hospita would be a promising candidate for Chinese privet biological control. C1 [Zhang, Yanzhuo; Jones, Cera] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Hanula, James L.; Horn, Scott] US Forest Serv, USDA, Southern Res Stn, 320 Green St, Athens, GA 30602 USA. [Braman, S. Kristine] Univ Georgia, Dept Entomol, Griffin, GA 30223 USA. [Sun, Jianghua] Chinese Acad Sci, Beijing 100101, Peoples R China. RP Zhang, YZ (reprint author), Univ Georgia, Dept Entomol, Athens, GA 30602 USA. EM yzzhang80@gmail.com; jhanula@fs.fed.us; shorn01@fs.fed.us; cejones318@gmail.com; kbrama-n@uga.edu; sunjh@ioz.ac.cn FU USDA Forest Service, Forest Health Technology Enterprise Team; Southern Research Station's Insects, Diseases, and Invasive Plants research unit FX We are grateful to Mike Cody for assistance in the quarantine laboratory. We also thank Richard Reardon (USDA Forest Service, Forest Health Technology Enterprise Team) and the Southern Research Station's Insects, Diseases, and Invasive Plants research unit for funding the research. NR 52 TC 0 Z9 0 U1 3 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 897 EP 908 DI 10.1093/ee/nvw062 PG 12 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600015 PM 27325627 ER PT J AU Giunta, AD Runyon, JB Jenkins, MJ Teich, M AF Giunta, A. D. Runyon, J. B. Jenkins, M. J. Teich, M. TI Volatile and Within-Needle Terpene Changes to Douglas-fir Trees Associated With Douglas-fir Beetle (Coleoptera: Curculionidae) Attack SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE bark beetle; terpene; wildfire; foliage; host attraction ID MOUNTAIN PINE-BEETLE; ORGANIC-COMPOUND EMISSIONS; BARK BEETLES; MONOTERPENE EMISSIONS; DENDROCTONUS PSEUDOTSUGAE; CONIFER-BARK; FLAMMABILITY; PLANT; TEMPERATURE; MORTALITY AB Mass attack by tree-killing bark beetles (Curculionidae: Scolytinae) brings about large chemical changes in host trees that can have important ecological consequences. For example, mountain pine beetle (Dendroctonus ponderosae Hopkins) attack increases emission of terpenes by lodgepole pine (Pinus contorta Dougl. ex Loud.), affecting foliage flammability with consequences for wildfires. In this study, we measured chemical changes to Douglas-fir (Pseudotsuga menziesii var. glauca (Mirb.) Franco) foliage in response to attack by Douglas-fir beetles (Dendroctonus pseudotsugae Hopkins) as trees die and crowns transitioned from green/healthy, to green-infested (year of attack), to yellow (year after attack), and red (2 yr after attack). We found large differences in volatile and within-needle terpene concentrations among crown classes and variation across a growing season. In general, emissions and concentrations of total and individual terpenes were greater for yellow and red needles than green needles. Douglas-fir beetle attack increased emissions and concentrations of terpene compounds linked to increased tree flammability in other conifer species and compounds known to attract beetles (e.g., a-pinene, camphene, and D-limonene). There was little relationship between air temperature or within-needle concentrations of terpenes and emission of terpenes, suggesting that passive emission of terpenes (e.g., from dead foliage) does not fully explain changes in volatile emissions. The potential physiological causes and ecological consequences of these bark beetle-associated chemical changes are discussed. C1 [Giunta, A. D.; Jenkins, M. J.; Teich, M.] Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. [Runyon, J. B.] US Forest Serv, USDA, Rocky Mt Res Stn, 1648 South 7th Ave, Bozeman, MT 59717 USA. RP Giunta, AD (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. EM adgiunta@gmail.com; jrunyon@fs.fed.us; mike.jenkins@usu.edu; michaela.teich@usu.edu FU Ecology Center at Utah State University; Utah Agricultural Experiment Station at Utah State University; Joint Fire Science Program [11-1-4-16] FX We give special thanks to Elizabeth Hebertson, and the staff of the USDA Forest Service, Forest Health Protection, Ogden Field Office, for their help with identifying study site locations, and to Wayne Beck, Caribou-Targhee NF, for study site approval. We also thank Jerrell Mock for assistance with data collection and Susan Durham for help with data analysis. We additionally thank Jose Negron and one anonymous reviewer for their feedback, which improved this manuscript. Support for this project was provided by the Ecology Center and the Utah Agricultural Experiment Station at Utah State University. Funding was provided by the Joint Fire Science Program (Project # 11-1-4-16). NR 70 TC 0 Z9 0 U1 14 U2 14 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 920 EP 929 DI 10.1093/ee/nvw040 PG 10 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600017 PM 27231258 ER PT J AU Cooper, WR Horton, DR Unruh, TR Garczynski, SF AF Cooper, W. Rodney Horton, David R. Unruh, Thomas R. Garczynski, Stephen F. TI Gut Content Analysis of a Phloem-Feeding Insect, Bactericera cockerelli (Hemiptera: Triozidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE landscape ecology; overwintering; tomato psyllid; Liberibacter psyllaurous ID CANDIDATUS LIBERIBACTER SOLANACEARUM; REAL-TIME PCR; STYLET PENETRATION; PACIFIC-NORTHWEST; UNITED-STATES; POTATO CROPS; EPG; DNA; HAPLOTYPES; PREDATORS AB Potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a key pest of potato (Solanum tuberosum L., Solanales: Solanaceae) and a vector of "Candidatus Liberibacter solanacearum," the pathogen associated with zebra chip disease. In addition to its presence on cultivated crops, the psyllid regularly occurs on numerous uncultivated annual and perennial species within the Solanaceae. A better understanding of landscape-level ecology of B. cockerelli would substantially improve our ability to predict which potato fields are most likely to be colonized by infected psyllids. We developed three PCR-based methods of gut content analysis to identify what plant species B. cockerelli had previously fed upon. These methods included-1) sequencing PCR amplicons of regions of plant-derived internal transcribed spacer (ITS) or the chloroplast trnL gene from psyllids, 2) high-resolution melting analysis of ITS or trnL real-time PCR products, and 3) restriction enzyme digestion of trnL PCR product. Each method was used to test whether we could identify psyllids that had been reared continuously on potato versus psyllids reared continuously on the perennial nightshade, Solanum dulcamara. All three methods of gut content analysis correctly identified psyllids from potato and psyllids from S. dulcamara. Our study is the first to demonstrate that plant DNA can be detected in a phloem-feeding insect. Gut content analysis, in combination with other landscape ecology approaches, could help elucidate patterns in landscape-level movements and host plant associations of B. cockerelli. C1 [Cooper, W. Rodney; Horton, David R.; Unruh, Thomas R.; Garczynski, Stephen F.] USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. RP Cooper, WR (reprint author), USDA ARS, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM rodney.cooper@ars.usda.gov; david.horton@ars.usda.gov; thomas.unruh@ars.usda.gov; steve.garczynski@ars.usda.gov RI Cooper, William/D-3205-2017 FU Northwest Potato Research Consortium; Washington State Committee for Pesticide Registration; National Institute of Food and Agriculture, U.S. Department of Agriculture, Specialty Crop Research Initiative [2015-09273] FX Heather Headrick, Pauline Anderson, Merilee Bayer, Debra Broers, and Glenda Torres provided technical assistance. Partial funding was provided by the Northwest Potato Research Consortium, the Washington State Committee for Pesticide Registration, and from National Institute of Food and Agriculture, U.S. Department of Agriculture, Specialty Crop Research Initiative award number 2015-09273. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 38 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 938 EP 944 DI 10.1093/ee/nvw060 PG 7 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600019 PM 27271944 ER PT J AU Shrestha, D McAuslane, HJ Adkins, ST Smith, HA Dufault, N Webb, SE AF Shrestha, D. McAuslane, H. J. Adkins, S. T. Smith, H. A. Dufault, N. Webb, S. E. TI Transmission of Squash vein yellowing virus to and From Cucurbit Weeds and Effects on Sweetpotato Whitefly (Hemiptera: Aleyrodidae) Behavior SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE insect-vector; virus source; susceptibility; weed; oviposition ID LEAF-CURL-VIRUS; BEMISIA-ARGENTIFOLII HOMOPTERA; MYZUS-PERSICAE HOMOPTERA; WATERMELON VINE DECLINE; DWARF VIRUS; 1ST REPORT; FEEDING-BEHAVIOR; TABACI HEMIPTERA; HAIRY NIGHTSHADE; INFECTED COTTON AB Since 2003, growers of Florida watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] have periodically suffered large losses from a disease caused by Squash vein yellowing virus (SqVYV), which is transmitted by the whitefly Middle East-Asia Minor 1 (MEAM1), formerly Bemisia tabaci (Gennadius) biotype B. Common cucurbit weeds like balsam apple (Momordica charantia L.) and smellmelon [Cucumis melo var. dudaim (L.) Naud.] are natural hosts of SqVYV, and creeping cucumber (Melothria pendula L.) is an experimental host. Study objectives were to compare these weeds and 'Mickylee' watermelon as sources of inoculum for SqVYV via MEAM1 transmission, to determine weed susceptibility to SqVYV, and to evaluate whitefly settling and oviposition behaviors on infected vs. mock-inoculated (inoculated with buffer only) creeping cucumber leaves. We found that the lowest percentage of watermelon recipient plants was infected when balsam apple was used as a source of inoculum. Watermelon was more susceptible to infection than balsam apple or smellmelon. However, all weed species were equally susceptible to SqVYV when inoculated by whitefly. For the first 5 h after release, whiteflies had no preference to settle on infected vs. mock-inoculated creeping cucumber leaves. After 24 h, whiteflies preferred to settle on mock-inoculated leaves, and more eggs were laid on mock-inoculated creeping cucumber leaves than on SqVYV-infected leaves. The transmission experiments (source of inoculum and susceptibility) show these weed species as potential inoculum sources of the virus. The changing settling preference of whiteflies from infected to mock-inoculated plants could lead to rapid spread of virus in the agroecosystem. C1 [Shrestha, D.; McAuslane, H. J.; Webb, S. E.] Univ Florida, Dept Entomol & Nematol, 1881 Nat Area Dr, Gainesville, FL 32611 USA. [Adkins, S. T.] ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. [Smith, H. A.] UF IFAS, Gulf Coast Res & Educ Ctr, 14625 Cty Rd 672, Wimauma, FL 33598 USA. [Dufault, N.] Univ Florida, Dept Plant Pathol, 2550 Hull Rd, Gainesville, FL 32611 USA. RP Shrestha, D (reprint author), Univ Florida, Dept Entomol & Nematol, 1881 Nat Area Dr, Gainesville, FL 32611 USA. EM dshrestha@u-fl.edu; hjmca@ufl.edu; Scott.Adkins@ars.usda.gov; hughasmith@ufl.edu; nsdufault@ufl.edu; sewe@ufl.edu FU USDA-ARS [58-6618-1-038]; University of Florida [58-6618-1-038]; University of Florida Graduate School FX We thank Linda Wessel-Beaver for providing creeping cucumber seeds and James Colee for advice on statistical analysis. Partial support was provided by a cooperative agreement (58-6618-1-038) between the USDA-ARS and the University of Florida. D.S. was supported by a fellowship provided by the University of Florida Graduate School. NR 69 TC 0 Z9 0 U1 12 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 967 EP 973 DI 10.1093/ee/nvw086 PG 7 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600023 PM 27400705 ER PT J AU Morrison, WR Lee, DH Reissig, WH Combs, D Leahy, K Tuttle, A Cooley, D Leskey, TC AF Morrison, William R., III Lee, Doo-Hyung Reissig, W. Harvey Combs, David Leahy, Kathleen Tuttle, Arthur Cooley, Daniel Leskey, Tracy C. TI Inclusion of Specialist and Generalist Stimuli in Attract-and-Kill Programs: Their Relative Efficacy in Apple Maggot Fly (Diptera: Tephritidae) Pest Management SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE integrated pest management; semiochemical; tephritid; attract and kill; Malus domestica ID RHAGOLETIS-POMONELLA DIPTERA; HOST FRUIT VOLATILES; PLANT VOLATILES; AMMONIUM CARBONATE; WASHINGTON-STATE; UNITED-STATES; FLIES DIPTERA; PLUM CURCULIO; SPHERES; TRAPS AB Investigating the chemical ecology of agricultural systems continues to be a salient part of integrated pest management programs. Apple maggot fly, a key pest of apple in eastern North America, is a visual specialist with attraction to host fruit-mimicking cues. These cues have been incorporated into red spherical traps used for both monitoring and behaviorally based management. Incorporating generalist or specialist olfactory cues can potentially increase the overall success of this management system. The primary aim of this study was to evaluate the attractiveness of a generalist olfactory cue, ammonium carbonate, and the specialist olfactory cue, a five-component apple volatile blend, when included as a component of a red attracticidal sphere system. Secondly, we assessed how critical it was to maintain minimal deviation from the optimal, full-round specialist visual stimulus provided by red spheres. Finally, attracticidal spheres were deployed with specialist olfactory cues in commercial apple orchards to evaluate their potential for effective management of apple maggot. Ammonium carbonate did not increase residency, feeding time, or mortality in the laboratory-based trials. Field deployment of specialist olfactory cues increased apple maggot captures on red spheres, while the generalist cue did not. Apple maggot tolerated some deviation from the optimal visual stimulus without reducing captures on red spheres. Attracticidal spheres hung in perimeter trees in orchards resulted in acceptable and statistically identical levels of control compared with standard insecticide programs used by growers. Overall, our study contributes valuable information for developing a reliable attract-and-kill system for apple maggot. C1 [Morrison, William R., III; Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. [Lee, Doo-Hyung] Gachon Univ, Dept Life Sci, Songnam, Kyeonggi Do, South Korea. [Reissig, W. Harvey; Combs, David] Cornell Univ, Dept Entomol, Geneva, NY USA. [Leahy, Kathleen] Polaris Orchard Management, Colrain, MA USA. [Tuttle, Arthur; Cooley, Daniel] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. RP Morrison, WR (reprint author), USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. EM william.morrison@ars.usda.gov; dl343@gachon.ac.kr; whr1@cornell.edu; dbc10@cornell.edu; polaris2@rcn.com; aft@umass.edu; dcooley@umass.edu; tracy.leskey@ars.usda.gov FU USDA NIFA [PMAP 2008-34381-19233] FX We would like to thank Torri Hancock and John Cullum for their excellent assistance. These studies were funded by USDA NIFA PMAP 2008-34381-19233 award. NR 56 TC 1 Z9 1 U1 12 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 974 EP 982 DI 10.1093/ee/nvw043 PG 9 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600024 PM 27330148 ER PT J AU Spears, LR Looney, C Ikerd, H Koch, JB Griswold, T Strange, JP Ramirez, RA AF Spears, Lori R. Looney, Chris Ikerd, Harold Koch, Jonathan B. Griswold, Terry Strange, James P. Ramirez, Ricardo A. TI Pheromone Lure and Trap Color Affects Bycatch in Agricultural Landscapes of Utah SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE beneficial insect; bumble bee; lady beetle; nontarget; bycatch ID FALL ARMYWORM LEPIDOPTERA; SEX-PHEROMONE; COLEOPTERA-COCCINELLIDAE; BUMBLEBEES HYMENOPTERA; NONTARGET HYMENOPTERA; HELICOVERPA-ARMIGERA; BEHAVIORAL-RESPONSES; NOCTUIDAE ADULTS; BAITED TRAPS; LADY BEETLE AB Aerial traps, using combinations of color and attractive lures, are a critical tool for detecting and managing insect pest populations. Yet, despite improvements in trap efficacy, collection of nontarget species ("bycatch") plagues many insect pest surveys. Bycatch can influence survey effectiveness by reducing the available space for target species and increasing trap screening time, especially in areas where thousands of insects are captured as bycatch in a given season. Additionally, bycatch may negatively impact local nontarget insect populations, including beneficial predators and pollinators. Here, we tested the effect of pheromone lures on bycatch rates of Coccinellidae (Coleoptera), Apoidea (Hymenoptera), and nontarget Lepidoptera. Multicolored (primarily yellow and white) bucket traps containing a pheromone lure for capturing one of three survey target species, Spodoptera litura (F.), S. littoralis (Boisduval), or Helicoverpa armigera (Hubner), were placed in alfalfa and corn fields, and compared to multicolored traps without a pheromone lure. All-green traps with and without H. armigera lures were employed in a parallel study investigating the effect of lure and trap color on bycatch. Over 2,600 Coccinellidae representing seven species, nearly 6,400 bees in 57 species, and >9,000 nontarget moths in 17 genera were captured across 180 traps and seven temporal sampling events. Significant effects of lure and color were observed for multiple taxa. In general, nontarget insects were attracted to the H. armigera lure and multicolored trap, but further studies of trap color and pheromone lure specificity are needed to better understand these interactions and to minimize nontarget captures. C1 [Spears, Lori R.; Ramirez, Ricardo A.] Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. [Looney, Chris] Washington State Dept Agr, 1111 Washington St SE, Olympia, WA 98504 USA. [Ikerd, Harold; Griswold, Terry; Strange, James P.] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Dept Biol, 5310 Old Main Hill, Logan, UT 84322 USA. [Koch, Jonathan B.] Univ Hawaii, Dept Biol, 200 W Kawili St, Hilo, HI 96720 USA. RP Spears, LR (reprint author), Utah State Univ, Dept Biol, 5305 Old Main Hill, Logan, UT 84322 USA. EM lori.spears@usu.edu; clooney@agr.wa.gov; Harold.Ikerd@ars.usda.gov; kochj@hawaii.edu; terry.griswold@ars.usda.gov; James.Strange@ars.usda.gov; ricardo.ramirez@usu.edu FU USDA-APHIS [14-8549-0587-CA, 13-8550-1248-CA]; Utah State University Extension grant FX We thank Sujaya Rao and four anonymous reviewers for reviewing earlier versions of this manuscript. We also thank Anna Fabiszak, DaShell Burnham, Steven Kuethe, Jaci Larson, Breanne Garrard, and Eric Hazelton for field and lab assistance, and the alfalfa and corn growers for allowing us access to their property during the survey. The alfalfa and corn commodity survey was funded in part by USDA-APHIS (#14-8549-0587-CA). Coleoptera and Hymenoptera processing was funded in part by a Utah State University Extension grant. Lepidoptera processing was funded in part by USDA-APHIS (#13-8550-1248-CA). NR 54 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 1009 EP 1016 DI 10.1093/ee/nvw085 PG 8 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600029 PM 27412193 ER PT J AU Frank, SD Ranger, CM AF Frank, Steven D. Ranger, Christopher M. TI Developing a Media Moisture Threshold for Nurseries to Reduce Tree Stress and Ambrosia Beetle Attacks SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Acer rubrum; Cornus florida; Magnolia grandiflora; ethanol; flood-stress ID COLEOPTERA-CURCULIONIDAE SCOLYTINAE; XYLOSANDRUS-GERMANUS COLEOPTERA; ETHANOL-BAITED TRAPS; BLACK-WALNUT; HOST-SELECTION; METABOLISM; MANAGEMENT; RESPONSES; EFFICACY; COMPLEX AB Exotic ambrosia beetles are among the most damaging pests of trees grown in nurseries. The primary pests Xylosandrus crassiusculus Motschulsky and Xylosandrus germanus Blandford use ethanol to locate vulnerable trees. Research, primarily with X. germanus, has shown that flood-stressed trees emit ethanol and are preferentially attacked by ambrosia beetles. Our goal was to develop a media (also called potting soil) moisture threshold as an integrated pest management (IPM) tactic and assess grower practices that lead to ambrosia beetle attacks. Flooded Cornus florida L., Cornus kousa Burg., and Magnolia grandiflora L. trees incurred more attacks than unflooded trees that were not attacked. To determine optimal media moisture levels, we grew flood-tolerant Acer rubrum L. and flood-intolerant C. florida in containers with 10, 30, 50, 70, or 90% media moisture. No flooded or unflooded A. rubrum were attacked. However, C. florida grown in 70 or 90% moisture were attacked and died, whereas trees at 30 and 50% moisture were not attacked. Thus, we suggest an upper moisture threshold of 50% when growing C. florida and other flood-intolerant trees. However, during peak ambrosia beetle flight activity in spring 2013 and 2014, we found that media moisture levels in commercial nurseries were often between 50 and 90%. Implementing a media moisture threshold, as a new IPM tool, could reduce ambrosia beetle attacks and the need for insecticide applications, which is currently the only available management tactic. Future research should focus on how changes in substrates, irrigation, and other practices could help growers meet this threshold. C1 [Frank, Steven D.] North Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA. [Ranger, Christopher M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Hort Insects Res Lab, 1680 Madison Ave, Wooster, OH 44691 USA. [Ranger, Christopher M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, 1680 Madison Ave, Wooster, OH 44691 USA. RP Frank, SD (reprint author), North Carolina State Univ, Dept Entomol, Campus Box 7613, Raleigh, NC 27695 USA. EM steven_frank@ncsu.edu; christopher.ranger@ars.usda.gov FU Horticultural Research Institute; North Carolina Nursery and Landscape Association; USDA Floriculture Nursery Research Initiative FX We thank Adcock Nursery, Currin's Nursery, Panther Creek Nursery, Stephenson's Nursery, Taylor's Nursery, and Swift Creek Nursery for allowing us to sample media moisture and trap ambrosia beetles. Adam Dale, Alan Stephenson, Greg Bryant, Andrew Ernst, and Laura Daly assisted with plant maintenance and data collection. We also thank Jenny Barnett (USDA-ARS) and Leslie Morris (USDA-ARS) for technical assistance with SPME-GC-MS analyses. This work was funded by grants from the Horticultural Research Institute and North Carolina Nursery and Landscape Association to SDF and funds from the USDA Floriculture Nursery Research Initiative to CMR. We thank two reviewers for comments that improved this manuscript. NR 39 TC 0 Z9 0 U1 2 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 1040 EP 1048 DI 10.1093/ee/nvw076 PG 9 WC Entomology SC Entomology GA DU0RG UT WOS:000381911600033 PM 27412195 ER PT J AU Hongthanat, N Kovar, JL Thompson, ML Russell, JR Isenhart, TM AF Hongthanat, Najphak Kovar, John L. Thompson, Michael L. Russell, James R. Isenhart, Thomas M. TI Phosphorus source-sink relationships of stream sediments in the Rathbun Lake watershed in southern Iowa, USA SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Stream sediments; Riparian pastures; Phosphorus losses; Degree of P saturation; Equilibrium P concentration ID MISSISSIPPI RIVER-BASIN; BANK EROSION; FLUVIAL SEDIMENT; GRAZED PASTURES; SURFACE WATERS; UNITED-STATES; SOIL; SORPTION; MEHLICH-3; NITROGEN AB The surface waters of Rathbun Lake watershed in southern Iowa are impacted by agricultural sources of sediments and nutrients, including phosphorus (P). Because stream sediments often play an important role in regulating P concentrations in stream water, we investigated sediment-water column P relationships in four creeks within the watershed and then evaluated the relationship between sediment properties and indicators of the risk of P loss. Based on Mehlich-3-extractable P (17 to 68 mg kg(-1)) and degree of P saturation (2 to 12%), stream bank and bed sediments at the four sites were unlikely to serve as major sources of P. However, equilibrium P concentrations, which ranged from 0.02 to 0.12 mg L-1, indicated that bed sediments could release P to the water column depending on dissolved P (DP) concentrations in the stream water and the time of year. The likelihood of P desorption from the sediments increased with increasing pH (r = 0.92, p < 0.01) and sand content (r = 0.78, p < 0.05), but decreased with clay content (r = -0.72, p< 0.05) and iron (Fe) (r = -0.93, p < 0.001) associated with organic matter. From these results, we speculate that changes in land use within the riparian areas may, at least initially, have little effect on P concentrations in the streams. Low concentrations of DP relative to total P (TP) in these streams, however, suggest that P loads to Rathbun Lake can be reduced if P inputs from eroded bank sediments are controlled. C1 [Hongthanat, Najphak; Thompson, Michael L.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Kovar, John L.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Russell, James R.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Isenhart, Thomas M.] Iowa State Univ, Nat Resource Ecol & Management Dept, Ames, IA 50011 USA. RP Kovar, JL (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM john.kovar@ars.usda.gov FU USDA Cooperative State Research, Education, and Extension Service 406 Program; Leopold Center for Sustainable Agriculture at Iowa State University FX The authors are grateful to Douglas Bear, Mustafa Tufekcioglu, Jay Berkey, and Teresita Chua for technical support. We also thank the USDA Cooperative State Research, Education, and Extension Service 406 Program and the Leopold Center for Sustainable Agriculture at Iowa State University for providing financial support. Mention of trade names does not imply recommendation or endorsement by Iowa State University or the USDA Agricultural Research Service. NR 62 TC 0 Z9 0 U1 16 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD AUG PY 2016 VL 188 IS 8 AR 453 DI 10.1007/s10661-016-5437-6 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA DT0AH UT WOS:000381144300010 PM 27393193 ER PT J AU Cooper, MG Nolin, AW Safeeq, M AF Cooper, Matthew G. Nolin, Anne W. Safeeq, Mohammad TI Testing the recent snow drought as an analog for climate warming sensitivity of Cascades snowpacks SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE climate change impacts; snow; warm spell; drought; temperature sensitivity; snow water equivalent ID WESTERN UNITED-STATES; DECLINING MOUNTAIN SNOWPACK; WASHINGTON CASCADES; NORTH-AMERICA; TRENDS; PRECIPITATION; TEMPERATURE; VARIABILITY; CALIFORNIA; HYDROLOGY AB Record low snowpack conditions were observed at Snow Telemetry stations in the Cascades Mountains, USA during the winters of 2014 and 2015. We tested the hypothesis that these winters are analogs for the temperature sensitivity of Cascades snowpacks. In the Oregon Cascades, the 2014 and 2015 winter air temperature anomalies were approximately +2 degrees C and +4 degrees C above the climatological mean. We used a spatially distributed snowpack energy balance model to simulate the sensitivity of multiple snowpack metrics to a +2 degrees C and +4 degrees C warming and compared our modeled sensitivities to observed values during 2014 and 2015. Wefound that for each +1 degrees C warming, modeled basin-mean peak snow water equivalent (SWE) declined by 22%-30%, the date of peak SWE (DPS) advanced by 13 days, the duration of snow cover (DSC) shortened by 31-34 days, and the snow disappearance date (SDD) advanced by 22-25 days. Our hypothesis was not borne out by the observations except in the case of peak SWE; other snow metrics did not resemble predicted values based on modeled sensitivities and thus are not effective analogs of future temperature sensitivities. Rather than just temperature, it appears that the magnitude and phasing of winter precipitation events, such as large, late spring snowfall, controlled the DPS, SDD, and DSC. C1 [Cooper, Matthew G.] Univ Calif Los Angeles, Dept Geog, 1255 Bunche Hall, Los Angeles, CA 90095 USA. [Cooper, Matthew G.] Oregon State Univ, Water Resources Grad Program, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Nolin, Anne W.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, 104 CEOAS Adm Bldg, Corvallis, OR 97331 USA. [Safeeq, Mohammad] Univ Calif, Sierra Nevada Res Inst, Merced, CA 95343 USA. [Safeeq, Mohammad] US Forest Serv, Pacific Southwest Res Stn, Fresno, CA 93710 USA. [Cooper, Matthew G.] Oregon State Univ, Water Resources Grad Program, Corvallis, OR 97331 USA. RP Cooper, MG (reprint author), Univ Calif Los Angeles, Dept Geog, 1255 Bunche Hall, Los Angeles, CA 90095 USA.; Cooper, MG (reprint author), Oregon State Univ, Water Resources Grad Program, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. EM guycooper@ucla.edu; nolina@oregonstate.edu; msafeeq@ucmerced.edu FU United States Geological Survey (USGS) Northwest Climate Science Center grant [G12AC20452]; College of Earth, Ocean, and Atmospheric Sciences, Oregon State University; Water Resources Graduate Program, Oregon State University FX This research was funded by United States Geological Survey (USGS) Northwest Climate Science Center grant G12AC20452. The first author thanks the College of Earth, Ocean, and Atmospheric Sciences and the Water Resources Graduate Program, Oregon State University for graduate student funding that supported the completion of this research. The authors thank Charlie Luce and two anonymous reviewers for their insightful and timely comments that significantly improved the quality of the manuscript. NR 51 TC 1 Z9 1 U1 10 U2 15 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD AUG PY 2016 VL 11 IS 8 AR 084009 DI 10.1088/1748-9326/11/8/084009 PG 11 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA DT9NH UT WOS:000381828300010 ER PT J AU McHugh, T AF McHugh, Tara TI Radio Frequency Processing of Food SO FOOD TECHNOLOGY LA English DT Editorial Material C1 [McHugh, Tara] ARS, USDA, Albany, CA 94710 USA. RP McHugh, T (reprint author), ARS, USDA, Albany, CA 94710 USA. NR 1 TC 0 Z9 0 U1 4 U2 4 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD AUG PY 2016 VL 70 IS 8 BP 73 EP 75 PG 3 WC Food Science & Technology SC Food Science & Technology GA DT8YC UT WOS:000381780600019 ER PT J AU Zinna, R Gotoh, H Brent, CS Dolezal, A Kraus, A Niimi, T Emlen, D Lavine, LC AF Zinna, R. Gotoh, H. Brent, C. S. Dolezal, A. Kraus, A. Niimi, T. Emlen, D. Lavine, L. C. TI Endocrine Control of Exaggerated Trait Growth in Rhinoceros Beetles SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Article ID JUVENILE-HORMONE TITERS; COPTOTERMES-FORMOSANUS ISOPTERA; JAPANESE HORNED BEETLES; ALLOMYRINA-DICHOTOMA; COLEOPTERA SCARABAEIDAE; CONDITIONAL EXPRESSION; ONTHOPHAGUS-TAURUS; SIGNALING PATHWAY; SEXUAL-DIMORPHISM; INSULIN-RECEPTOR AB Juvenile hormone (JH) is a key insect growth regulator frequently involved in modulating phenotypically plastic traits such as caste determination in eusocial species, wing polymorphisms in aphids, and mandible size in stag beetles. The jaw morphology of stag beetles is sexually-dimorphic and condition-dependent; males have larger jaws than females and those developing under optimum conditions are larger in overall body size and have disproportionately larger jaws than males raised under poor conditions. We have previously shown that large males have higher JH titers than small males during development, and ectopic application of fenoxycarb (JH analog) to small males can induce mandibular growth similar to that of larger males. What remains unknown is whether JH regulates condition-dependent trait growth in other insects with extreme sexually selected structures. In this study, we tested the hypothesis that JH mediates the condition-dependent expression of the elaborate horns of the Asian rhinoceros beetle, Trypoxylus dichotomus. The sexually dimorphic head horn of this beetle is sensitive to nutritional state during larval development. Like stag beetles, male rhinoceros beetles receiving copious food produce disproportionately large horns for their body size compared with males under restricted diets. We show that JH titers are correlated with body size during the late feeding and early prepupal periods, but this correlation disappears by the late prepupal period, the period of maximum horn growth. While ectopic application of fenoxycarb during the third larval instar significantly delayed pupation, it had no effect on adult horn size relative to body size. Fenoxycarb application to late prepupae also had at most a marginal effect on relative horn size. We discuss our results in context of other endocrine signals of condition-dependent trait exaggeration and suggest that different beetle lineages may have co-opted different physiological signaling mechanisms to achieve heightened nutrient-sensitive weapon growth. C1 [Zinna, R.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Gotoh, H.] Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan. [Brent, C. S.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Dolezal, A.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Kraus, A.] Gonzaga Univ, Dept Biol, Spokane, WA 99258 USA. [Niimi, T.] Natl Inst Basic Biol, Div Evolutionary Dev Biol, Okazaki, Aichi 4448585, Japan. [Emlen, D.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Zinna, R (reprint author), Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. EM lavine@wsu.edu RI Gotoh, Hiroki/B-7568-2017 NR 78 TC 1 Z9 1 U1 9 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD AUG PY 2016 VL 56 IS 2 BP 247 EP 259 DI 10.1093/icb/icw042 PG 13 WC Zoology SC Zoology GA DT1YX UT WOS:000381279100012 PM 27252223 ER PT J AU Srygley, RB AF Srygley, R. B. TI Diet Drives the Collective Migrations and Affects the Immunity of Mormon Crickets and Locusts: A Comparison of These Potential Superspreaders of Disease SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Article ID SIMPLEX ORTHOPTERA TETTIGONIIDAE; APOLIPOPHORIN-III; ANABRUS-SIMPLEX; ADIPOKINETIC HORMONE; MIGRATORY LOCUST; FUNGAL-INFECTION; INSECT; CANNIBALISM; POPULATIONS; DENSITY AB Differential transmission of disease among individuals within a population or among species in a community can result in superspreaders, relatively rare individuals responsible for a large proportion of transmission events. Migrating Mormon crickets and nymphal locusts readily engage in cannibalistic attacks and necrophagy. Typically multiple individuals consume a cadaver, which fosters the spread of disease. Cannibalistic attacks result in aligned, coordinated movement of individuals in massive bands that march daily for weeks at a time. Coordinated movement reduces contact frequency, which not only reduces cannibalism but the risk of disease transmission. When crowded, Mormon crickets and locusts elevate their constitutive immunity, which further reduces the risk of disease transmission. Bands of Mormon crickets show a variety of macronutrient dietary deficiencies that determine whether they will be more susceptible to pathogenic bacteria or fungi. In some migratory bands, Mormon crickets seek carbohydrates and have less anti-bacterial activity. A lipid transport protein that functions in both fuelling migration and anti-bacterial activity may cause a trade-off between the two activities when carbohydrates are limited. In other migratory bands, Mormon crickets prefer protein over carbohydrates, indicating protein-deficiency. In these bands, the generalized immunity of Mormon crickets, measured as phenoloxidase, is compromised, and the insects are more susceptible to Beauveria bassiana fungal infection. In locusts, a high protein diet resulted in greater susceptibility to another entomopathogenic fungus, Metarhizium acridum, whereas in Mormon crickets, both phenoloxidase titers and immunity to M. acridum increased with adult age. Color changes associated with death by either of these fungi diminishes cannibalism, but bands may cull infected or encounter cadavers too quickly to effectively reduce fungal transmission. As long as the insects show no signs of infection that ward off their conspecifics, then infected Mormon crickets and locusts in migratory bands could be superspreaders of disease. However, the diseases that they are most likely to harbor and amplify may depend on the dietary deficiencies exhibited by members of the band. C1 [Srygley, R. B.] ARS, Pest Management Res Unit, Northern Plains Agr Res Lab, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. RP Srygley, RB (reprint author), ARS, Pest Management Res Unit, Northern Plains Agr Res Lab, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. EM robert.srygley@ars.usda.gov NR 55 TC 0 Z9 0 U1 12 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 EI 1557-7023 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD AUG PY 2016 VL 56 IS 2 BP 268 EP 277 DI 10.1093/icb/icw035 PG 10 WC Zoology SC Zoology GA DT1YX UT WOS:000381279100014 PM 27252211 ER PT J AU Gall, CA Reif, KE Scoles, GA Mason, KL Mousel, M Noh, SM Brayton, KA AF Gall, Cory A. Reif, Kathryn E. Scoles, Glen A. Mason, Kathleen L. Mousel, Michelle Noh, Susan M. Brayton, Kelly A. TI The bacterial microbiome of Dermacentor andersoni ticks influences pathogen susceptibility SO ISME JOURNAL LA English DT Article ID ANAPLASMA-MARGINALE; AMBLYOMMA-AMERICANUM; BOOPHILUS-MICROPLUS; VECTOR COMPETENCE; TRANSMISSION; INFECTION; IXODIDAE; ACARI; IDENTIFICATION; RICKETTSIALES AB Ticks are of medical importance owing to their ability to transmit pathogens to humans and animals. The Rocky Mountain wood tick, Dermacentor andersoni, is a vector of a number of pathogens, including Anaplasma marginale, which is the most widespread tick-borne pathogen of livestock. Although ticks host pathogenic bacteria, they also harbor bacterial endosymbionts that have a role in tick physiology, survival, as well as pathogen acquisition and transmission. The goal of this study was to characterize the bacterial microbiome and examine the impact of microbiome disruption on pathogen susceptibility. The bacterial microbiome of two populations of D. andersoni with historically different susceptibilities to A. marginale was characterized. In this study, the microbiome was disrupted and then ticks were exposed to A. marginale or Francisella novicida to determine whether the microbiome correlated with pathogen susceptibility. Our study showed that an increase in proportion and quantity of Rickettsia bellii in the microbiome was negatively correlated to A. marginale levels in ticks. Furthermore, a decrease in Francisella endosymbionts was associated with lower F. novicida infection levels, demonstrating a positive pathogen-endosymbiont relationship. We demonstrate that endosymbionts and pathogens have varying interactions, and suggest that microbiome manipulation may provide a possible method for biocontrol by decreasing pathogen susceptibility of ticks. C1 [Gall, Cory A.; Noh, Susan M.; Brayton, Kelly A.] Washington State Univ, Dept Vet Microbiol & Pathol, Bustad Rm 402, Pullman, WA 99164 USA. [Reif, Kathryn E.; Scoles, Glen A.; Mason, Kathleen L.; Mousel, Michelle; Noh, Susan M.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Noh, Susan M.; Brayton, Kelly A.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. RP Brayton, KA (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Bustad Rm 402, Pullman, WA 99164 USA. EM kbrayton@vetmed.wsu.edu OI Mousel, Michelle/0000-0003-1367-7005 FU National Institutes of Health [AI044005, AI093524, 5T32GM008336-25]; USDA-ARS-CRIS [5348-32000-033-00D]; CVM intramural funds FX We thank Lisa Orfe, Mark Wildung, Derek Pouchnik, Ralph Horn, James Allison and the staff at the USDA-ARS in Moscow, ID, USA for excellent guidance and technical assistance. This research was supported by National Institutes of Health grants, AI044005, AI093524, 5T32GM008336-25, www.experiment.com (crowdsourcing grant), USDA-ARS-CRIS 5348-32000-033-00D and CVM intramural funds. NR 35 TC 1 Z9 1 U1 14 U2 15 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 EI 1751-7370 J9 ISME J JI ISME J. PD AUG PY 2016 VL 10 IS 8 BP 1846 EP 1855 DI 10.1038/ismej.2015.266 PG 10 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA DS7JN UT WOS:000380959800005 PM 26882265 ER PT J AU Singer, E Bushnell, B Coleman-Derr, D Bowman, B Bowers, RM Levy, A Gies, EA Cheng, JF Copeland, A Klenk, HP Hallam, SJ Hugenholtz, P Tringe, SG Woyke, T AF Singer, Esther Bushnell, Brian Coleman-Derr, Devin Bowman, Brett Bowers, Robert M. Levy, Asaf Gies, Esther A. Cheng, Jan-Fang Copeland, Alex Klenk, Hans-Peter Hallam, Steven J. Hugenholtz, Philip Tringe, Susannah G. Woyke, Tanja TI High-resolution phylogenetic microbial community profiling SO ISME JOURNAL LA English DT Article ID 16S RIBOSOMAL-RNA; GENE-SEQUENCES; DARK-MATTER; DIVERSITY; BACTERIAL; PRIMERS; CLASSIFICATION; OXIDATION; TAXONOMY; GENOMES AB Over the past decade, high-throughput short-read 16S rRNA gene amplicon sequencing has eclipsed clone-dependent long-read Sanger sequencing for microbial community profiling. The transition to new technologies has provided more quantitative information at the expense of taxonomic resolution with implications for inferring metabolic traits in various ecosystems. We applied single-molecule real-time sequencing for microbial community profiling, generating full-length 16S rRNA gene sequences at high throughput, which we propose to name PhyloTags. We benchmarked and validated this approach using a defined microbial community. When further applied to samples from the water column of meromictic Sakinaw Lake, we show that while community structures at the phylum level are comparable between PhyloTags and Illumina V4 16S rRNA gene sequences (iTags), variance increases with community complexity at greater water depths. PhyloTags moreover allowed less ambiguous classification. Last, a platform-independent comparison of PhyloTags and in silico generated partial 16S rRNA gene sequences demonstrated significant differences in community structure and phylogenetic resolution across multiple taxonomic levels, including a severe underestimation in the abundance of specific microbial genera involved in nitrogen and methane cycling across the Lake's water column. Thus, PhyloTags provide a reliable adjunct or alternative to cost-effective iTags, enabling more accurate phylogenetic resolution of microbial communities and predictions on their metabolic potential. C1 [Singer, Esther; Bushnell, Brian; Coleman-Derr, Devin; Bowers, Robert M.; Levy, Asaf; Cheng, Jan-Fang; Copeland, Alex; Tringe, Susannah G.; Woyke, Tanja] US DOE, Joint Genome Inst, 2800 Mitchell Dr, Walnut Creek, CA 94598 USA. [Coleman-Derr, Devin] USDA ARS, Albany, CA USA. [Bowman, Brett] Pacific Biosci, Menlo Pk, CA USA. [Gies, Esther A.; Hallam, Steven J.] Univ British Columbia, Vancouver, BC, Canada. [Klenk, Hans-Peter] Newcastle Univ, Sch Biol, Newcastle Upon Tyne, Tyne & Wear, England. [Hugenholtz, Philip] Univ Queensland, Australian Ctr Ecogen, Sch Chem & Mol Biosci, St Lucia, Qld, Australia. [Hugenholtz, Philip] Univ Queensland, Inst Mol Biosci, St Lucia, Qld, Australia. RP Tringe, SG; Woyke, T (reprint author), US DOE, Joint Genome Inst, 2800 Mitchell Dr, Walnut Creek, CA 94598 USA. EM sgtringe@lbl.gov; twoyke@lbl.gov FU University of British Columbia; Canadian Institute for Advanced Research (CIFAR); Tula Foundation funded Centre for Microbial Diversity and Evolution (CMDE); Natural Sciences and Engineering Research Council (NSERC) of Canada; Canada Foundation of Innovation (CFI); US Department of Energy Joint Genome Institute; DOE Office of Science User Facility [DE-AC02-05CH11231] FX We thank the JGI production team for assistance in sequencing and Doina Ciobanu for the generation of the mock community. This work was performed under the auspices of the US Department of Energy Joint Genome Institute, a DOE Office of Science User Facility with funding support from Contract No. DE-AC02-05CH11231, the Natural Sciences and Engineering Research Council (NSERC) of Canada, Canada Foundation of Innovation (CFI), the Tula Foundation funded Centre for Microbial Diversity and Evolution (CMDE) and the Canadian Institute for Advanced Research (CIFAR) through grants awarded to SJH. EAG was supported by a 4-year fellowship (4YF) from the University of British Columbia. NR 48 TC 6 Z9 6 U1 17 U2 21 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 EI 1751-7370 J9 ISME J JI ISME J. PD AUG PY 2016 VL 10 IS 8 BP 2020 EP 2032 DI 10.1038/ismej.2015.249 PG 13 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA DS7JN UT WOS:000380959800019 PM 26859772 ER PT J AU Susta, L Dimitrov, KM Miller, PJ Brown, CC Afonso, CL AF Susta, Leonardo Dimitrov, Kiril M. Miller, Patti J. Brown, Corrie C. Afonso, Claudio L. TI Reply to "May Newly Defined Subgenotypes Va and Vb of Newcastle Disease Virus in Poultry Be Considered Two Different Genotypes?" SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Letter C1 [Susta, Leonardo] Univ Guelph, Dept Pathobiol, Guelph, ON, Canada. [Dimitrov, Kiril M.; Miller, Patti J.; Afonso, Claudio L.] ARS, Southeast Poultry Res Lab, USDA, Athens, GA USA. [Brown, Corrie C.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. RP Susta, L (reprint author), Univ Guelph, Dept Pathobiol, Guelph, ON, Canada. EM lsusta@uoguelph.ca NR 6 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 EI 1098-660X J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD AUG PY 2016 VL 54 IS 8 BP 2205 EP 2206 DI 10.1128/JCM.00914-16 PG 2 WC Microbiology SC Microbiology GA DT1YO UT WOS:000381278200044 PM 27458270 ER PT J AU Ben Moussa, IE Mazzoni, V Valentini, F Yaseen, T Lorusso, D Speranza, S Digiaro, M Varvaro, L Krugner, R D'Onghia, AM AF Ben Moussa, Issam Eddine Mazzoni, Valerio Valentini, Franco Yaseen, Thaer Lorusso, Donato Speranza, Stefano Digiaro, Michele Varvaro, Leonardo Krugner, Rodrigo D'Onghia, Anna Maria TI Seasonal Fluctuations of Sap-Feeding Insect Species Infected by Xylella fastidiosa in Apulian Olive Groves of Southern Italy SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Philaenus spumarius; Auchenorrhyncha; vector; plant disease; PCR ID GLASSY-WINGED SHARPSHOOTER; LEAF SCORCH DISEASE; PIERCES DISEASE; CAUSAL AGENT; BACTERIUM; TRANSMISSION; VECTORS; CULTURE; IDENTIFICATION; PATHOGENICITY AB A study on seasonal abundance of Auchenorrhyncha species and their infectivity by Xylella fastidiosa in the Apulia region of Italy was conducted to identify ideal periods for monitoring and adoption of potential control measures against insect vectors. Adult populations of Auchenorrhyncha species were monitored monthly over a 2-yr period from five olive groves. A total of 15 species were captured, identified, and tested for presence of X. fastidiosa by polymerase chain reaction (PCR). For three species, Philaenus spumarius L., Neophilaenus campestris (Fallen), and Euscelis lineolatus Brulle, positive reactions to X. fastidiosa were obtained, on average, in 16.3, 15.9 and 18.4% of adult insects, respectively. Philaneous spumarius was the dominant species (39.8% of total Auchenorrhyncha captured) with the highest adult abundance in summer months. Adult P. spumarius and N. campestris were first detected between March and May in both years, and all insects tested during these periods (year 1: n = 42, year 2: n = 132) gave negative reactions to X. fastidiosa by PCR. Similarly, first adults of E. lineolatus that appeared from October to November (year 1: n = 20, year 2: n = 15) tested negative for presence of X. fastidiosa. Given the lack of transstadial and transovarial transmission of X. fastidiosa and considering that P. spumarius is univoltine, control measures against nymphal stages of P. spumarius should be investigated as means of population suppression to reduce spread of X. fastidiosa in olive groves. C1 [Ben Moussa, Issam Eddine; Speranza, Stefano; Varvaro, Leonardo] Univ Tuscia, Dipartimento Sci & Tecnol Agr Foreste Nat & Energ, Viterbo, Italy. [Ben Moussa, Issam Eddine; Valentini, Franco; Yaseen, Thaer; Lorusso, Donato; Digiaro, Michele; D'Onghia, Anna Maria] CIHEAM Ist Agron Mediterraneo, Valenzano, BA, Italy. [Mazzoni, Valerio] Fdn Edmund Mach, Ctr Ric & Innovaz, San Michele All Adige, TN, Italy. [Krugner, Rodrigo] USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. RP D'Onghia, AM (reprint author), CIHEAM Ist Agron Mediterraneo, Valenzano, BA, Italy. EM benmoussa@unitus.it; valerio.mazzoni@fmach.it; valentini@iamb.it; y.thaer@iamb.it; xdonato2@gmail.com; speranza@unitus.it; digiaro@iamb.it; varvaro@unitus.it; Rodrigo.Krugner@ars.usda.gov; donghia@iamb.it OI Speranza, Stefano/0000-0003-0106-3938 NR 28 TC 0 Z9 0 U1 4 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1512 EP 1518 DI 10.1093/jee/tow123 PG 7 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900003 PM 27401111 ER PT J AU Golec, JR Duan, JJ Aparicio, E Hough-Goldstein, J AF Golec, Julian R. Duan, Jian J. Aparicio, Ellen Hough-Goldstein, Judith TI Life History, Reproductive Biology, and Larval Development of Ontsira mellipes (Hymenoptera: Braconidae), a Newly Associated Parasitoid of the Invasive Asian Longhorned Beetle (Coleoptera: Cerambycidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Anoplophora glabripennis; developmental biology; reproductive biology; new-association biocontrol; life history ID ANOPLOPHORA-GLABRIPENNIS COLEOPTERA; HARDWOOD FORESTS; UNITED-STATES; NORTH-AMERICA; HOST LOCATION; FRUIT-FLIES; BEAN WEEVIL; LEPIDOPTERA; BEHAVIOR; WASPS AB The invasive Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), is a destructive xylophagous forest pest species originating from Asia. Several endemic North American hymenopteran (Braconidae) species in the mid-Atlantic region were capable of attacking and reproducing on A. glabripennis larvae in laboratory bio-assays. Ontsira mellipes Ashmead (Hymenoptera: Braconidae) has been continually reared on A. glabripennis larvae at USDA-ARS BIIRU since 2010, and has been identified as a potential new-association biocontrol agent. Two experiments were conducted to investigate parasitism, paralysis, reproductive biology, larval development, and longevity of adult O. mellipes. In the first experiment, pairs of adult parasitoids were given single A. glabripennis larvae every 2 d (along with honey and water) over their lifetimes, while in the second experiment individual parasitoids were observed daily from egg to adult, and adults were subsequently starved. Adults in the first experiment parasitized similar to 21% of beetle larvae presented to them throughout their life, and paralysis of larvae occurred 1-2 d after oviposition. More than half of the individual pairs parasitized A. glabripennis larvae, with each female producing around 26 offspring throughout her life. In the second experiment, median development time of O. mellipes from egg to adult was about 3 wk, with five larval instars. Adult O. mellipes that were provided with host larvae, honey, and water lived 9 d longer than host-deprived and starved adults. These findings indicate that mass-rearing procedures for O. mellipes may be developed using the new association host for development of effective biocontrol programs against A. glabripennis. C1 [Golec, Julian R.; Hough-Goldstein, Judith] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA. [Golec, Julian R.; Duan, Jian J.; Aparicio, Ellen] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. RP Golec, JR (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA.; Golec, JR (reprint author), ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM juliang@udel.edu; jian.duan@ars.usda.gov; ellen.aparicio@ars.usda.gov; jhough@udel.edu FU USDA Forest Service International Research Program; USDA Agricultural Research Service FX We thank Daria Tatman and Linda Saunders (USDA-ARS BIIRU) for their help in rearing and providing A. glabripennis for the study, and Joe Tropp (USDA-ARS-BIIRU) for providing the red maple sticks. Additionally, we thank the anonymous reviewers for their useful comments and suggestions that helped to greatly improve this manuscript. This project is supported by the USDA Forest Service International Research Program and USDA Agricultural Research Service. NR 92 TC 0 Z9 0 U1 17 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1545 EP 1554 DI 10.1093/jee/tow122 PG 10 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900008 PM 27329634 ER PT J AU Tang, JH Cheng, YX Sappington, TW Jiang, XF Zhang, L Luo, LZ AF Tang, Jihong Cheng, Yunxia Sappington, Thomas W. Jiang, Xingfu Zhang, Lei Luo, Lizhi TI Egg Hatch and Survival and Development of Beet Webworm (Lepidoptera: Crambidae) Larvae at Different Combinations of Temperature and Relative Humidity SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Loxostege sticticalis; survival rate; development; temperature; relative humidity ID LOXOSTEGE-STICTICALIS LEPIDOPTERA; PYRALIDAE; SURVIVORSHIP; MIGRATION; LONGEVITY; CHINA; L. AB To understand the role that temperature and humidity play in the population dynamics of the beet webworm, Loxostege sticticalis L. (Lepidoptera: Crambidae), egg hatch, survival of first-fifth instars, survival of the full larval stage, survival curves, and larval development rates were investigated at combinations of four temperatures (18, 22, 26, and 30 degrees C) and five relative humidities (RH; 20%, 40%, 60%, 80%, and 100%). We found that greatest egg hatch rate, survival rates of the first and second instars, and survival rate of the complete larval stage occurred at 22 degrees C and 60-80% RH; the lowest values for these parameters were observed at 30 degrees C and 20% RH. Survival of first instars was significantly affected by the interaction of temperature and relative humidity. However, survival of third and fourth instars was neither affected by temperature nor relative humidity, and that of fifth instars was significantly affected only by relative humidity level. The survival curve for larvae was well described by a type III Weibull distribution. Duration of larval stage decreased as temperature increased, but was not affected by relative humidity. We therefore conclude that eggs and early instars are the most critical stages for survival to the pupal stage, and 22-26 degrees C and 60-80% RH are the optimum conditions for their survival and development. These findings confirm that temperature and relative humidity are the critical environmental factors affecting the population growth of L. sticticalis, with temperature being more important. C1 [Tang, Jihong; Cheng, Yunxia; Jiang, Xingfu; Zhang, Lei; Luo, Lizhi] CAAS, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Sappington, Thomas W.] Iowa State Univ, USDA ARS, Genet Lab, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Luo, LZ (reprint author), CAAS, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM jihong_23@163.com; yxcheng@ippcaas.cn; Tom.Sappington@ars.usda.gov; xfjiang@ippcaas.cn; leizhang@ippcaas.cn; lzluo@ippcaas.cn FU Special Fund for Agro-Scientific Research in the Public Interest [201303057, 201403031]; International Science & Technology Cooperation Program of China [2014DFR31250]; National Natural Science Foundation of China [31301656, 31371947] FX This work was financially supported by the Special Fund for Agro-Scientific Research in the Public Interest (201303057; 201403031), International Science & Technology Cooperation Program of China (2014DFR31250), the National Natural Science Foundation of China (31301656; 31371947). NR 35 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1603 EP 1611 DI 10.1093/jee/tow106 PG 9 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900015 PM 27329620 ER PT J AU Bernklau, EJ Hibbard, BE Norton, AP Bjostad, LB AF Bernklau, E. J. Hibbard, B. E. Norton, A. P. Bjostad, L. B. TI Methyl Anthranilate as a Repellent for Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diabrotica virgifera virgifera; Zea mays; methyl anthranilate; host location; repellent ID BEHAVIORAL-RESPONSES; HEADSPACE VOLATILES; FEEDING STIMULANTS; PARASITIC WASPS; CARBON-DIOXIDE; ATTRACTANT; MAIZE; SEMIOCHEMICALS; STRATEGIES; MANAGEMENT AB Methyl anthranilate was identified as the active compound in extracts of maize (Zea mays L.) roots that were shown to be repellent to neonate western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae. A bio-assay-driven approach was used to isolate the active material from diethyl ether extracts of roots from germinating maize seeds. Separation of the extract on a Florisil column yielded an active fraction of 90: 10 hexane: diethyl ether. Analysis with gas chromatography-mass spectrometry identified two compounds in the active fraction: indole (2,3-benzopyrrole) and methyl anthranilate (methyl 2-aminobenzoate). When tested in behavioral bioassays, methyl anthranilate elicited a significant (P < 0.05) repellent response at doses of 1, 10, and 100 mu g. In subsequent single-choice bioassays, 1, 10, and 100 mu g of methyl anthranilate prevented larvae from approaching 10 mmol/mol concentrations of carbon dioxide, which is normally highly attractive to the larvae. Indole, the other compound identified from the active fraction, did not elicit a behavioral response by the larvae. Methyl anthranilate has potential for development as a management tool for western corn rootworm larvae and may be best suited for use in a push-pull control strategy. C1 [Bernklau, E. J.; Norton, A. P.; Bjostad, L. B.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Hibbard, B. E.] Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. RP Bernklau, EJ (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. EM ebernklau@mac.com; Bruce.Hibbard@ars.usda.gov; andrew.norton@colostate.edu; louis.bjostad@colostate.edu FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2014-67013-21764]; Colorado Agricultural Experiment Station [622] FX We thank Julie Barry (USDA-ARS, Columbia, MO) for assistance in western corn rootworm colony rearing and for shipments of eggs for experiments. We thank the USDA-ARS North Central Regional Plant Introduction Station for providing maize seed for evaluation. This project was supported by the Agriculture and Food Research Initiative Competitive Grant no. 2014-67013-21764 from the USDA National Institute of Food and Agriculture and the Colorado Agricultural Experiment Station (project number 622). NR 56 TC 1 Z9 1 U1 8 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1683 EP 1690 DI 10.1093/jee/tow090 PG 8 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900025 PM 27122493 ER PT J AU Sullivan, BT Brownie, C Barrett, JP AF Sullivan, Brian T. Brownie, Cavell Barrett, JoAnne P. TI Intra-Annual Variation in Responses by Flying Southern Pine Beetles (Coleoptera: Curculionidae: Scolytinae) to Pheromone Component endo-Brevicomin SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE monitoring; bark beetle; trapping; pheromone; phenology ID DENDROCTONUS-FRONTALIS ZIMMERMANN; GREAT-LAKES REGION; BARK BEETLES; POPULATION-DYNAMICS; IPS-TYPOGRAPHUS; PEST-MANAGEMENT; EAST TEXAS; AGGREGATION PHEROMONE; BEHAVIORAL CHEMICALS; SEASONAL-VARIATIONS AB The southern pine beetle Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae: Scolytinae) is attracted to an aggregation pheromone that includes the multifunctional pheromone component endo-brevicomin. The effect of endo-brevicomin on attractive lures varies from strong enhancement to reduction of beetle attraction depending upon release rate, lure component spacing, and proximity of beetle infestations. Anecdotal observations have further suggested that the effects of endo-brevicomin vary during the year. We investigated this possibility under nonoutbreak conditions in southwestern Mississippi where for two-and-a-half years we monitored traps baited with frontalin and the host odor alpha-pinene either (a) alone, or with an endo-brevicomin release device either (b) located directly on the trap, or (c) displaced 6 m away. The endo-brevicomin devices in our tests increased D. frontalis catches during all times of year, and 6 m displacement of the endo-brevicomin release device from the trap did not significantly alter responses except during the spring flight peak when displacement increased catches. Our data suggest that flying D. frontalis have a stronger tendency to avoid the immediate proximity of a release point of endo-brevicomin during their springtime dispersal flight when catches are greatest. Catches of Thanasimus dubius (F.) (Coleoptera: Cleridae), a major predator of D. frontalis, were not altered by endo-brevicomin, and ratios of D. frontalis to T. dubius changed over the course of the year. We discuss the possible effects of intra-annual variation in D. frontalis response to endo-brevicomin both on beetle attack behavior and use of endo-brevicomin as a lure adjuvant in D. frontalis population monitoring. C1 [Sullivan, Brian T.; Barrett, JoAnne P.] US Forest Serv, USDA, Southern Res Stn, 2500 Shreveport Hwy, Pineville, LA 71360 USA. [Brownie, Cavell] North Carolina State Univ, 2311 Stinson Dr, Raleigh, NC 27695 USA. RP Sullivan, BT (reprint author), US Forest Serv, USDA, Southern Res Stn, 2500 Shreveport Hwy, Pineville, LA 71360 USA. EM briansullivan@fs.fed.us; browniecavell@gmail.com; jbarrett@fs.fed.us FU USDA Forest Service Southern Research Station [RWU-4552] FX We wish to thank the Homochitto National Forest and in particular Lee Dunnam for use of and assistance with the research sites. We also thank Zach Oliver and Chris Young for field assistance. Drs. Ronald Billings, Stephen Clarke, Robert Coulson, and William Shepherd reviewed early drafts of the manuscript. Funding was provided by USDA Forest Service Southern Research Station RWU-4552. NR 64 TC 0 Z9 0 U1 4 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1720 EP 1728 DI 10.1093/jee/tow078 PG 9 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900030 PM 27207264 ER PT J AU Helbig, CE Coyle, DR Klepzig, KD Nowak, JT Gandhi, KJK AF Helbig, Christiane E. Coyle, David R. Klepzig, Kier D. Nowak, John T. Gandhi, Kamal J. K. TI Colonization Dynamics of Subcortical Insects on Forest Sites With Relatively Stressed and Unstressed Loblolly Pine Trees SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Curculionidae; pine decline; Pinus taeda; rhizophagous beetle; Scolytinae ID WEEVILS COLEOPTERA-CURCULIONIDAE; PACHYLOBIUS-PICIVORUS COLEOPTERA; BLACK TURPENTINE BEETLE; ETHANOL-BAITED TRAPS; CENTRAL ALABAMA; FUNGAL COMPLEX; AMBROSIA BEETLES; SOUTHERN PINES; HYLOBIUS-PALES; LONGLEAF PINE AB Loblolly pine (Pinus taeda L.) is the most important commercial tree species in the southeastern United States. Since the 1950s, there have been reports of loblolly pines showing reduced growth and increased mortality, particularly in central Alabama and western Georgia, United States; the phenomenon is termed as southern pine decline (SPD). Recently, the role of rhizophagous (root-feeding) insects in loblolly pine health within the context of SPD has come under greater scrutiny. We investigated the impacts of subcortical insects, particularly rhizophagous weevils (Coleoptera: Curculionidae), on loblolly pine health in northeastern Georgia. We created plots-representing a gradient of increased relative tree stress-from ungirdled trees, ungirdled trees baited with ethanol and turpentine (ungirdled-baited), and girdled trees. In total, 10,795 subcortical insects from four families (Buprestidae, Cerambycidae, Curculionidae, and Siricidae) and >82 species were trapped in two years. Almost half of the insects trapped (46% of individuals and 11% of species) were nonnative to North America. Insect captures in plots with girdled trees were 61 and 187% greater than those with ungirdled-baited and ungirdled trees, respectively. Tree treatment impacted captures of native, but not nonnative insects. Relative feeding area by the rhizophagous weevils Hylobius pales (Herbst) and Pachylobius picivorus (Germar) on pine twigs placed in pitfall traps was 1, 17, and 82% in plots with ungirdled, ungirdled-baited, and girdled trees, respectively. Hence, there was a strong association of native subcortical insects, especially rhizophagous weevils, with relatively highly stressed trees, confirming that they are secondary instead of primary pine colonizers. C1 [Helbig, Christiane E.] Tech Univ Dresden, Professorship Forest Protect, Pienner Str 8, D-01737 Tharandt, Germany. [Helbig, Christiane E.; Coyle, David R.; Gandhi, Kamal J. K.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA. [Klepzig, Kier D.] US Forest Serv, USDA, Southern Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. [Nowak, John T.] US Forest Serv, USDA, Forest Hlth Protect, 200 WT Weaver Blvd, Asheville, NC 28804 USA. RP Gandhi, KJK (reprint author), Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, 180 E Green St, Athens, GA 30602 USA. EM Chr.Helbig@web.de; drcoyle@uga.edu; kklepzig@fs.fed.us; jnowak@fs.fed.us; kjgandhi@uga.edu FU USDA Forest Service, Forest Health Protection and Southern Research Station; Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia FX We thank B. Barnes, C. Brissey, and K. Smoot (University of Georgia) for field and laboratory assistance, and R. Hoebeke (University of Georgia) and D. Bright (Colorado State University) for their support in identifying the insect species. We are also grateful to three anonymous reviewers whose comments improved the paper. Funding was provided by the USDA Forest Service, Forest Health Protection and Southern Research Station, and Daniel B. Warnell School of Forestry and Natural Resources, University of Georgia. NR 79 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1729 EP 1740 DI 10.1093/jee/tow083 PG 12 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900031 PM 27252398 ER PT J AU Zukoff, SN Zukoff, AL Geisert, RW Hibbard, BE AF Zukoff, Sarah N. Zukoff, Anthony L. Geisert, Ryan W. Hibbard, Bruce E. TI Western Corn Rootworm (Coleoptera: Chrysomelidae) Larval Movement in eCry3.1Ab+mCry3A Seed Blend Scenarios SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bt resistance; Bacillus thuringiensis; Diabrotica virgifera virgifera; insect resistance management ID VIRGIFERA-VIRGIFERA COLEOPTERA; TO-PLANT MOVEMENT; TRANSGENIC CORN; MAIZE; FIELD; ESTABLISHMENT; OVIPOSITION; ADAPTATION; RESISTANCE; EMERGENCE AB Corn fields planted with plant-incorporated Bacillus thuringiensis (Bt) proteins must have a portion of the field planted with non-Bt, isoline, plants that serve as a refuge for susceptible insects. In the Corn Belt, refuge seeds are now blended in the bag with Bt seeds for corn hybrids containing two or more toxins targeted toward the western corn rootworm, Diabrotica virgifera virgifera LeConte. Syngenta's corn hybrid, Agrisure Duracade, containing the eCry3.1Ab (event 5307) and mCry3a (event MIR604) rootworm-targeted toxins were registered as a seed blend in 2014. Western corn rootworm larval movement between the refuge plants and the Duracade plants was assessed to determine western corn rootworm survival and amount of root damage on these plants when planted in all possible seed blend scenarios. In this study, western corn rootworm larvae moved between isoline and Bt plants and adult survival was greater on Bt plants if movement from a neighboring infested isoline plant had occurred. However, root damage to these Bt plants did not reach economic levels. The low numbers of western corn rootworm larvae that did move from an infested Bt plant to an isoline plant could potentially select for resistance if they survived to adulthood. C1 [Zukoff, Sarah N.; Zukoff, Anthony L.] Kansas State Univ, Southwest Res & Extens Ctr, 4500 Mary St, Garden City, KS 67846 USA. [Geisert, Ryan W.] USDA ARS, 1503 S Providence,Res PK, Columbia, MO 65211 USA. [Hibbard, Bruce E.] Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. RP Zukoff, SN (reprint author), Kansas State Univ, Southwest Res & Extens Ctr, 4500 Mary St, Garden City, KS 67846 USA. EM snzukoff@ksu.edu; azukoff@ksu.edu; rwg5h8@mail.missouri.edu; Bruce.Hibbard@ars.usda.gov FU Syngenta Company FX We thank Dr. Ivan Hiltpolt, Julie Barry, and a number of temporary laborers for technical assistance. We thank Syngenta Company for providing seed and funding for this project. NR 38 TC 1 Z9 1 U1 7 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1834 EP 1845 DI 10.1093/jee/tow046 PG 12 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900045 PM 27190042 ER PT J AU Liu, J Lincoln, T An, JJ Gao, ZL Dang, ZH Pan, WL Li, YF AF Liu, Jia Lincoln, Tamra An, Jingjie Gao, Zhanlin Dang, Zhihong Pan, Wenliang Li, Yaofa TI The Joint Toxicity of Different Temperature Coefficient Insecticides on Apolygus lucorum (Hemiptera: Miridae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE insecticide; temperature effect; joint toxicity; Apolygus lucorum ID POSTTREATMENT TEMPERATURE; ORGANOPHOSPHATE INSECTICIDES; BT COTTON; PYRETHROIDS; LEPIDOPTERA; PERMETHRIN; NOCTUIDAE; CURCULIONIDAE; COLEOPTERA; SYNERGISM AB The effect of temperature on the cotoxicity coefficient (CTC) value was used to evaluate mixture efficacy of different temperature coefficient chemicals from 15 to 35 degrees C by exposing third-instar Apolygus lucorum (MeyerDur) to dip-treated asparagus bean pods. The results indicated the joint toxicity of same temperature coefficient insecticide (TCI) types were unaffected by temperature. This means that even when temperatures change, the mixture ratios of the highest CTC values remained the same, and the effect of temperature on the joint toxicity of same TCI types was only on the CTC values. However, the effect of temperature was variable when considering the joint toxicity of different TCI types. The effect of temperature on the joint toxicity of both strong positive and strong negative TCI types was clear, and the highest CTC values of mixture ratios changed with temperature regularly. When comparing the influence of temperature between strong/slight positive/negative insecticides, the results indicated a greater influence of the strong TCI. Paradoxically, the highest CTC value of the imidacloprid and methomyl mixture did not change with temperature changes consistently, even with the variance of imidacloprid ratios, a strong TCI. These results will guide pest managers in choosing the most effective insecticide mixtures for A. lucorum control under given environmental conditions. C1 [Liu, Jia; An, Jingjie; Gao, Zhanlin; Dang, Zhihong; Pan, Wenliang; Li, Yaofa] Hebei Acad Agr & Forestry Sci, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov, Plant Protect Inst,Minist Agr, Baoding 07100, Peoples R China. [Lincoln, Tamra] USDA ARS, BCIRL, 1503 S Providence,Res Pk, Columbia, MO USA. RP Li, YF (reprint author), Hebei Acad Agr & Forestry Sci, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov, Plant Protect Inst,Minist Agr, Baoding 07100, Peoples R China. EM 15031210252@126.com; trfy9f@mail.missouri.edu; anjingjie147@163.com; gaozhanlin@sina.com; dangzhihong@sina.com; pwenliang@163.com; liyaofa@gmail.com FU National Natural Science Foundation of China [31201553]; Special Fund for Agro-scientific Research in the Public Interest [201103012] FX This project was supported by the National Natural Science Foundation of China (31201553) and the Special Fund for Agro-scientific Research in the Public Interest (201103012). We are grateful to Dr. David W. Stanley for helpful comments on this manuscript. There is no conflict of interest among the authors. NR 36 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1846 EP 1852 DI 10.1093/jee/tow082 PG 7 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900046 PM 27190041 ER PT J AU Geisert, RW Hibbard, BE AF Geisert, Ryan W. Hibbard, Bruce E. TI Evaluation of Potential Fitness Costs Associated With eCry3.1Ab Resistance in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bt resistance; Bacillus thuringiensis; insect resistance management ID WESTERN CORN-ROOTWORM; FIELD-EVOLVED RESISTANCE; BACILLUS-THURINGIENSIS; TRANSGENIC-MAIZE; INSECT RESISTANCE; BT MAIZE; MORTALITY; NEBRASKA; LARVAE; PEST AB Both an eCry3.1Ab-selected and paired control western corn rootworm, Diabrotica virgifera virgifera LeConte, colony were tested for adult longevity, egg oviposition, egg viability, and larval development in order to evaluate the potential fitness costs associated with eCry3.1Ab resistance. Adult longevity experiments were conducted by pairing virgin males and females together in plastic boxes supplied with food, water, and ovipositional medium and observed for survival time. Eggs were also collected from the ovipositional medium once a week to determine average egg oviposition and egg viability. Larval development time experiments were conducted by infesting seedling assays with 25 neonate larvae and recording larval recovery after several days. Adult longevity, average egg oviposition, and larval development time results indicated a lack of fitness costs associated with eCry3.1Ab resistance in the western corn rootworm. Results of egg viability indicated a fitness advantage for the eCry3.1Ab-selected colony with a significantly higher egg hatch than the control. C1 [Geisert, Ryan W.] USDA ARS, 1503 S Providence,Res PK, Columbia, MO 65211 USA. [Hibbard, Bruce E.] Univ Missouri, USDA ARS, 205 Curtis Hall, Columbia, MO 65211 USA. RP Geisert, RW (reprint author), USDA ARS, 1503 S Providence,Res PK, Columbia, MO 65211 USA. EM rwg5h8@mail.missouri.edu; Bruce.Hibbard@ars.usda.gov FU Syngenta Biotechnology FX We thank Julie Barry (USDA-ARS) for technical assistance in rearing parental colonies and suggestions with the reciprocal cross colonies. We thank Syngenta Biotechnology for providing funding, Bt and near-isoline seed. NR 47 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1853 EP 1858 DI 10.1093/jee/tow095 PG 6 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900047 PM 27151470 ER PT J AU Gassmann, AJ Shrestha, RB Jakka, SRK Dunbar, MW Clifton, EH Paolino, AR Ingber, DA French, BW Masloski, KE Dounda, JW St Clair, CR AF Gassmann, Aaron J. Shrestha, Ram B. Jakka, Siva R. K. Dunbar, Mike W. Clifton, Eric H. Paolino, Aubrey R. Ingber, David A. French, B. Wade Masloski, Kenneth E. Dounda, John W. St Clair, Coy R. TI Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE bioassay; Diabrotica virgifera virgifera; insect resistance management; integrated pest management; refuge strategy ID VIRGIFERA-VIRGIFERA LECONTE; ERROR COMPONENT MODEL; BT MAIZE; EVOLVED RESISTANCE; EVENT DAS-59122-7; TRANSGENIC MAIZE; MANAGEMENT; ECOLOGY; CROPS; INSECTICIDES AB Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States, and recent management of western corn rootworm has included planting of Bt corn. Beginning in 2009, western corn rootworm populations with resistance to Cry3Bb1 corn and mCry3A corn were found in Iowa and elsewhere. To date, western corn rootworm populations have remained susceptible to corn producing Bt toxin Cry34/35Ab1. In this study, we used single-plant bioassays to test field populations of western corn rootworm for resistance to Cry34/35Ab1 corn, Cry3Bb1 corn, and mCry3A corn. Bioassays included nine rootworm populations collected from fields where severe injury to Bt corn had been observed and six control populations that had never been exposed to Bt corn. We found incomplete resistance to Cry34/35Ab1 corn among field populations collected from fields where severe injury to corn producing Cry34/35Ab1, either singly or as a pyramid, had been observed. Additionally, resistance to Cry3Bb1 corn and mCry3A corn was found among the majority of populations tested. These first cases of resistance to Cry34/35Ab1 corn, and the presence of resistance to multiple Bt toxins by western corn rootworm, highlight the potential vulnerability of Bt corn to the evolution of resistance by western corn rootworm. The use of more diversified management practices, in addition to insect resistance management, likely will be essential to sustain the viability of Bt corn for management of western corn rootworm. C1 [Gassmann, Aaron J.; Shrestha, Ram B.; Jakka, Siva R. K.; Dunbar, Mike W.; Clifton, Eric H.; Paolino, Aubrey R.; Ingber, David A.; Masloski, Kenneth E.; Dounda, John W.; St Clair, Coy R.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Jakka, Siva R. K.] Valent USA Co, 3800 Old Leland Rd, Leland, MS 38756 USA. [Ingber, David A.] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19716 USA. [French, B. Wade] USDA ARS, North Cent Agr Res Lab, Brookings, SD USA. RP Gassmann, AJ (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM aaronjg@iastate.edu; shrestrb@iastate.edu; sjakk@valent.com; dunbar@iastate.edu; eclifton@iastate.edu; apaolino@iastate.edu; davidngbr@gmail.com; wade.french@ars.usda.gov; masloski@iastate.edu; jdoudna@iastate.edu; cstclair@iasta-te.edu FU Biotechnology Risk Assessment Grant Program from the USDA National Institute of Food and Agriculture [2012-33522-20010] FX We thank Dow AgroSciences, Monsanto, and Syngenta for providing the corn seed used to conduct plant-based bioassays, and Chad Nielson, Deb Patterson, and several students for technical assistance with control populations. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This research was supported by Biotechnology Risk Assessment Grant Program competitive grant 2012-33522-20010 from the USDA National Institute of Food and Agriculture. NR 49 TC 3 Z9 3 U1 11 U2 16 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1872 EP 1880 DI 10.1093/jee/tow110 PG 9 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900049 PM 27329619 ER PT J AU Paudyal, S Opit, GP Arthur, FH Bingham, GV Gautam, SG AF Paudyal, S. Opit, G. P. Arthur, F. H. Bingham, G. V. Gautam, S. G. TI Contact Toxicity of Deltamethrin Against Tribolium castaneum (Coleoptera: Tenebrionidae), Sitophilus oryzae (Coleoptera: Curculionidae), and Rhyzopertha dominica (Coleoptera: Bostrichidae) Adults SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE knockdown; bagged grain; red flour beetle; rice weevil; lesser grain borer ID STORED-PRODUCT BEETLES; PESTS; INSECTICIDES; CONCRETE; SURFACES; INSECTS; STORAGE; AFRICA; LOSSES; GRAINS AB This study was conducted at Oklahoma State University, Stillwater, OK, to evaluate the response to deltamethrin concentrations for adults of three stored-product insects, Tribolium castaneum (Herbst), Sitophilus oryzae (L.), and Rhyzopertha dominica (F.). In insect toxicological studies, knockdown is the state of intoxication and partial paralysis as a result of exposure to an insecticide. Deltamethrin concentrations ranging from 0.48 to 140 mg/m(2) (1 to 3,000 ppm) were sprayed on glass Petri dishes. After the dishes dried, 20 adult insects of each species were placed on the treated dishes to determine the contact toxicity of deltamethrin. Assessments for knockdown were made at 15-min intervals for up to 8 h after initial exposure and then again after 24 or 48 h. Insects were then transferred to clean untreated Petri dishes with diet and observed from 0.5 to 72 h. Mortality was assessed 72 h after transfer to untreated dishes with food material. Deltamethrin was highly effective against all three species tested and achieved 99% knockdown of insects of all species within 4 h after exposure at concentrations >= 1.2 mg/m(2). Although some insects recovered from initial knockdown at concentrations <= 48 mg/m(2), nearly all the insects were killed at 140 mg/m(2) when exposed for 48 h. LC95 values for all species tested, for the 48-h exposure period, were < 140 mg/m(2), the concentration of deltamethrin that could potentially be present in new ZeroFly Storage Bag fabric. ZeroFly bags are used for stored-product insect pest control. C1 [Paudyal, S.; Opit, G. P.; Gautam, S. G.] Oklahoma State Univ, Dept Entomol & Plant Pathol, 127 Noble Res Ctr, Stillwater, OK 74078 USA. [Arthur, F. H.] USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. [Bingham, G. V.] Vestergaard SA, Pl St Francois 1, CH-1003 Lausanne, Switzerland. [Gautam, S. G.] Kearney Agr Res & Extens Ctr, 9240 S Riverbend Ave, Parlier, CA 93648 USA. RP Opit, GP (reprint author), Oklahoma State Univ, Dept Entomol & Plant Pathol, 127 Noble Res Ctr, Stillwater, OK 74078 USA. EM sulochana.paudyal@okstate.edu; george.opit@okstate.edu; frank.arthur@ars.usda.gov; gvb@vestergaard.com; sandipa.gautam@okstate.edu FU Vestergaard SA, Lausanne, Switzerland; Oklahoma Agricultural Experiment Station [OKL2949] FX We thank Kandara Shakva, Friendly Yang at Oklahoma State University in Stillwater OK, and Brian Barnett at the USDA-ARS-Center for Grain and Animal Health Research in Manhattan, KS, for their technical support. This work was funded by Vestergaard SA, Lausanne, Switzerland; partial funding for the work came from the Oklahoma Agricultural Experiment Station (Project No. OKL2949). This paper reports the results of research only. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by Oklahoma State University, Vestergaard SA, Switzerland, or the US Department of Agriculture. The USDA and Oklahoma State University are equal opportunity employers and providers. NR 36 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2016 VL 109 IS 4 BP 1936 EP 1942 DI 10.1093/jee/tow107 PG 7 WC Entomology SC Entomology GA DU0TH UT WOS:000381916900057 PM 27270576 ER PT J AU Griffin, MJ Reichley, SR Greenway, TE Quiniou, SM Ware, C Gao, DX Gaunt, PS Yanong, RPE Pouder, DB Hawke, JP Soto, E AF Griffin, M. J. Reichley, S. R. Greenway, T. E. Quiniou, S. M. Ware, C. Gao, D. X. Gaunt, P. S. Yanong, R. P. E. Pouder, D. B. Hawke, J. P. Soto, E. TI Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins SO JOURNAL OF FISH DISEASES LA English DT Article DE Edwardsiella; catfish; tilapia; zebrafish ID POLYMERASE-CHAIN-REACTION; MICROBIAL GENE IDENTIFICATION; CAUSATIVE AGENT; REACTION ASSAY; SEQUENCE; CATFISH; FISH; DIVERSITY; STRAINS; MODELS AB The intraspecific variability of E. ictaluri isolates from different origins was investigated. Isolates were recovered from farm-raised catfish (Ictalurus punctatus) in Mississippi, USA, tilapia (Oreochromis niloticus) cultured in the Western Hemisphere and zebrafish (Danio rerio) propagated in Florida, USA. These isolates were phenotypically homologous and antimicrobial profiles were largely similar. Genetically, isolates possessed differences that could be exploited by repetitive-sequence-mediated PCR and gyrB sequence, which identified three distinct E. ictaluri genotypes: one associated with catfish, one from tilapia and a third from zebrafish. Plasmid profiles were also group specific and correlated with rep-PCR and gyrB sequences. The catfish isolates possessed profiles typical of those described for E. ictaluri isolates; however, plasmids from the zebrafish and tilapia isolates differed in both composition and arrangement. Furthermore, some zebrafish and tilapia isolates were PCR negative for several E. ictaluri virulence factors. Isolates were serologically heterogenous, as serum from a channel catfish exposed to a catfish isolate had reduced antibody activity to tilapia and zebrafish isolates. This work identifies three genetically distinct strains of E. ictaluri from different origins using rep-PCR, 16S, gyrB and plasmid sequencing, in addition to antimicrobial and serological profiling. C1 [Griffin, M. J.; Reichley, S. R.; Greenway, T. E.; Ware, C.; Gao, D. X.; Gaunt, P. S.] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, POB 197,127 Expt Stn Rd, Stoneville, MS 39762 USA. [Quiniou, S. M.] ARS, Thad Cochran Natl Warmwater Aquaculture Ctr, Warmwater Aquaculture Res Unit, USDA, Stoneville, MS USA. [Yanong, R. P. E.; Pouder, D. B.] Univ Florida, Trop Aquaculture Lab, Sch Forest Resources & Conservat, Fisheries & Aquat Sci Program,Inst Food & Agr Sci, Ruskin, FL USA. [Hawke, J. P.] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA. [Soto, E.] Ross Univ, Sch Vet Med, Dept Biomed Sci, Basseterre, St Kitts, W Ind Assoc St. RP Griffin, MJ (reprint author), Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, POB 197,127 Expt Stn Rd, Stoneville, MS 39762 USA. EM matt.griffin@msstate.edu OI Reichley, Stephen/0000-0002-4537-4335 FU United States Department of Agriculture Catfish Health Initiative [CRIS 6402-31320-002-02]; National Institute of Food and Agriculture; U.S. Department of Agriculture [MIS-371530]; Mississippi State University College of Veterinary Medicine; Mississippi Agriculture and Forestry Experiment Station; Ross University Center for Conservation Medicine and Ecosystem Health FX This work was supported by the United States Department of Agriculture Catfish Health Initiative (CRIS 6402-31320-002-02), the National Institute of Food and Agriculture, U.S. Department of Agriculture, Project No. MIS-371530, the Mississippi State University College of Veterinary Medicine, the Mississippi Agriculture and Forestry Experiment Station and the Ross University Center for Conservation Medicine and Ecosystem Health. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement. This is MAFES publication #J-12725. NR 39 TC 0 Z9 0 U1 3 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7775 EI 1365-2761 J9 J FISH DIS JI J. Fish Dis. PD AUG PY 2016 VL 39 IS 8 BP 947 EP 969 DI 10.1111/jfd.12431 PG 23 WC Fisheries; Marine & Freshwater Biology; Veterinary Sciences SC Fisheries; Marine & Freshwater Biology; Veterinary Sciences GA DS6WE UT WOS:000380923000004 PM 26661707 ER PT J AU Harris, LJ Lieberman, V Mashiana, RP Atwill, E Yang, M Chandler, JC Bisha, B Jones, T AF Harris, Linda J. Lieberman, Vanessa Mashiana, Rupinder P. Atwill, Edward Yang, Mai Chandler, Jeffrey C. Bisha, Bledar Jones, Thomas TI Prevalence and Amounts of Salmonella Found on Raw California Inshell Pistachios SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Nuts; Pistachios; Salmonella concentration; Salmonella levels; Salmonella prevalence; Salmonella survey ID ALMONDS; ENTERITIDIS; LEVEL AB After harvest, pistachios are hulled with mechanical abrasion and then separated in a float tank containing water; the nuts that float (similar to 15%; floaters) and those that sink (similar to 85%; sinkers) are dried and stored separately. To determine the prevalence of Salmonella in pistachios, a total of 3,966 samples (1,032 floaters and 2,934 sinkers) were collected within 4 months of the 2010, 2011, and 2012 harvests from storage silos (12 samples from each silo, in most cases) and were stored at 4 degrees C; 100-g subsamples were enriched for the presence of Salmonella. Twenty-one of the floater samples and 11 of the sinker samples were positive for Salmonella: 2.0% prevalence (95% confidence interval [CI], 1.3 to 3.1%) and 0.37% prevalence (95% CI, 0.21 to 0.67%), respectively, for a weighted average prevalence of 0.61%. Levels of Salmonella were determined for positive samples using a most-probable-number (MPN) method with multiple 50-g, three 5.6-g, and three 0.56-g subsamples. Geometric mean levels of Salmonella in floaters and sinkers were 0.66 MPN/100 g (0.14 to 5.3 MPN/100 g) and 0.18 MPN/100 g (0.10 to 0.62 MPN/100 g), respectively. Seven different serovars were identified among the isolates, with nine pulsed-field gel electrophoresis fingerprints; as many as four serovars were isolated from some samples. Salmonella serovars Montevideo (44%), Enteritidis (19%), Senftenberg (16%), Worthington (12%), and Liverpool (9.4%) were most commonly isolated from the initial 100-g samples. The prevalence and levels of Salmonella in pistachios are within those observed for other tree nuts, but the limited number of serovars isolated suggests a narrow and persistent contamination source. C1 [Harris, Linda J.; Lieberman, Vanessa; Mashiana, Rupinder P.] Univ Calif Davis, Dept Food Sci & Technol, One Shields Ave, Davis, CA 95616 USA. [Harris, Linda J.; Atwill, Edward] Univ Calif Davis, Western Ctr Food Safety, One Shields Ave, Davis, CA 95616 USA. [Atwill, Edward] Univ Calif Davis, Dept Populat Hlth & Reprod Med, One Shields Ave, Davis, CA 95616 USA. [Yang, Mai; Jones, Thomas] DFA Calif, 1855 South Van Ness Ave, Fresno, CA 93721 USA. [Chandler, Jeffrey C.; Bisha, Bledar] Univ Wyoming, Dept Anim Sci, 1000 East Univ Ave, Laramie, WY 82071 USA. [Chandler, Jeffrey C.] USDA, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Harris, LJ (reprint author), Univ Calif Davis, Dept Food Sci & Technol, One Shields Ave, Davis, CA 95616 USA.; Harris, LJ (reprint author), Univ Calif Davis, Western Ctr Food Safety, One Shields Ave, Davis, CA 95616 USA. EM ljharris@ucdavis.edu RI Harris, Linda/B-5030-2011 OI Harris, Linda/0000-0002-1911-752X FU Center for Produce Safety at the University of California, Davis; California Department of Food and Agriculture; California Pistachio Research Board FX Funds were provided by the Center for Produce Safety at the University of California, Davis, in partnership with the California Department of Food and Agriculture's Specialty Crop Block Grant Program, and the California Pistachio Research Board. The contributions made by Lillian Khan, Chris Theofel, and Sylvia Yada in technical support and during the writing process are greatly appreciated. NR 33 TC 1 Z9 1 U1 1 U2 4 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2016 VL 79 IS 8 BP 1304 EP 1315 DI 10.4315/0362-028X.JFP-16-054 PG 12 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DT7GR UT WOS:000381655600002 PM 27497117 ER PT J AU Ukuku, DO Mukhopadhyay, S Geveke, D Olanya, M Niemira, B AF Ukuku, Dike O. Mukhopadhyay, Sudarsan Geveke, David Olanya, Modesto Niemira, Brendan TI Effect of Hydrogen Peroxide in Combination with Minimal Thermal Treatment for Reducing Bacterial Populations on Cantaloupe Rind Surfaces and Transfer to Fresh-Cut Pieces SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Bacterial attachment; Cantaloupe rind structure; Decontamination ID ESCHERICHIA-COLI O157-H7; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; EXTRACELLULAR-MATRIX; STORAGE-TEMPERATURE; MICROBIAL QUALITY; NATIVE MICROFLORA; ATTACHMENT; HYDROPHOBICITY; SURVIVAL AB Surface structure and biochemical characteristics of bacteria and produce play a major role in how and where bacteria attach, complicating decontamination treatments. Whole cantaloupe rind surfaces were inoculated with Salmonella, Escherichia coli 0157:H7, and Listeria monocytogenes at 107 CFU/ml. Average population size of Salmonella, Escherichia coli 0157:H7, and L. monocytogenes recovered after surface inoculation was 4.8 +/- 0.12, 5.1 +/- 0.14, and 3.6 +/- 0.13 log CFU/cm(2), respectively. Inoculated melons were stored at 5 and 22 degrees C for 7 days before washing treatment interventions. Intervention treatments used were (i) water (H(2)0) at 22 degrees C, (ii) H2O at 80 degrees C, (iii) 3% hydrogen peroxide (H2O2) at 22 degrees C, and (iv) a combination of 3% H2O2 and H2O at 80 degrees C for 300 s. The strength of pathogen attachment (S-R value) at days 0, 3, and 7 of storage was determined, and then the efficacy of the intervention treatments to detach, kill, and reduce transfer of bacteria to fresh -cut pieces during fresh -cut preparation was investigated. Populations of E. coli O157:H7 attached to the rind surface at significantly higher levels (P < 0.05) than Salmonella and L. monocytogenes, but Salmonella exhibited the strongest attachment (SR value) at all days tested. Washing with 3% 11202 alone led to significant reduction (P < 0.05) of bacteria and caused some changes in bacterial cell morphology. A combination treatment with H2O and 3% H2O2 at 80 degrees C led to an average 4-log reduction of bacterial pathogens, and no bacterial pathogens were detected in fresh-cut pieces prepared from this combination treatment, including enriched fresh-cut samples. The results of this study indicate that the microbial safety of fresh-cut pieces from treated cantaloupes was improved at day 6 of storage at 5 degrees C and day 3 of storage at 10 degrees C. C1 [Ukuku, Dike O.; Mukhopadhyay, Sudarsan; Geveke, David; Olanya, Modesto; Niemira, Brendan] ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. RP Ukuku, DO (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM dike.ukuku@ars.usda.gov NR 37 TC 1 Z9 1 U1 6 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2016 VL 79 IS 8 BP 1316 EP 1324 DI 10.4315/0362-028X.JFP-16-046 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DT7GR UT WOS:000381655600003 PM 27497118 ER PT J AU Bosilevac, JM Wang, R Luedtke, BE Wheeler, TL Koohmaraie, M AF Bosilevac, Joseph M. Wang, Rong Luedtke, Brandon E. Wheeler, Tommy L. Koohmaraie, Mohammad TI Contamination Revealed by Indicator Microorganism Levels during Veal Processing SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Aerobic plate counts; Carcass; Enterobacteriaceae counts; Escherichia coli and coliform counts; Hide; Veal ID ESCHERICHIA-COLI O157-H7; AEROBIC-BACTERIA; PREVALENCE; CARCASSES; BEEF; HIDES; PLANT; ENTEROBACTERIACEAE; SLAUGHTER; INTERVENTIONS AB During site visits of veal processors, the U.S. Department of Agriculture, Food Safety Inspection Service (FSIS) has reported processing deficiencies that likely contribute to increased levels of veal contamination. Here, we report the results of measuring aerobic plate count bacteria (APC), Enterobacteriaceae, coliforms (CF), and Escherichia coli during eight sample collections at five veal processors to assess contamination during the harvest of bob veal and formula-fed veal before (n = 5 plants) and after (n =3 plants) changes to interventions and processing practices. Hides of veal calves at each plant had mean log CFU/100 cm(2) APC, Enterobacteriaceae, CF, and E. coli of 6.02 to 8.07, 2.95 to 5.24, 3.28 to 5.83, and 3.08 to 5.59, respectively. Preintervention carcasses had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 3.08 to 5.22, 1.16 to 3.47, 0.21 to 3.06, and 0.07 to 3.10, respectively, before and 2.72 to 4.50, 0.99 to 2.76, 0.69 to 2.26, and 0.33 to 2.12, respectively, after changes were made to improve sanitary dressing procedures. Final veal carcasses had mean log CFU/100 cm2 APC, Enterobacteriaceae, CF, and E. coli of 0.36 to 2.84, -0.21 to 1.59, -0.23 to 1.59, and -0.38 to 1.45 before and 0.44 to 2.64, -0.16 to 1.33, -0.42 to 1.20, and -0.48 to 1.09 after changes were made to improve carcass-directed interventions. Whereas the improved dressing procedures resulted in improved carcass cleanliness, the changes to carcass-directed interventions were less successful, and veal processors are urged to use techniques that ensure uniform and consistent delivery of antimicrobials to carcasses. Analysis of results comparing bob veal to formula-fed veal found bob veal hides, preintervention carcasses, and final carcasses to have increased (P < 0.05) APC, Enterobacteriaceae, CF, and E. coli (with the exception of hide Enterobacteriaceae", P > 0.05) relative to formula fed veal. When both veal categories were harvested at the same plant on the same day, similar results were observed. Since identification by FSIS, the control of contamination during veal processing has started to improve, but challenges still persist. C1 [Bosilevac, Joseph M.; Wang, Rong; Luedtke, Brandon E.; Wheeler, Tommy L.] ARS, USDA, US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. [Koohmaraie, Mohammad] IEH Labs & Consulting Grp, 15300 Bothell Way NE, Lake Forest Pk, WA 98155 USA. [Luedtke, Brandon E.] Univ Nebraska Kearney, 905 West 25th St, Kearney, NE 68849 USA. RP Bosilevac, JM (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM mick.bosilevac@ars.usda.gov NR 14 TC 1 Z9 1 U1 2 U2 2 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2016 VL 79 IS 8 BP 1341 EP 1347 DI 10.4315/0362-028X.JFP-15-572 PG 7 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DT7GR UT WOS:000381655600006 PM 27497121 ER PT J AU Beier, RC Franz, E Bono, JL Mandrell, RE Fratamico, PM Callaway, TR Andrews, K Poole, TL Crippen, TL Sheffield, CL Anderson, RC Nisbet, DJ AF Beier, Ross C. Franz, Eelco Bono, James L. Mandrell, Robert E. Fratamico, Pina M. Callaway, Todd R. Andrews, Kathleen Poole, Toni L. Crippen, Tawni L. Sheffield, Cynthia L. Anderson, Robin C. Nisbet, David J. TI Disinfectant and Antimicrobial Susceptibility Profiles of the Big Six Non-0157 Shiga Toxin-Producing Escherichia coli Strains from Food Animals and Humans SO JOURNAL OF FOOD PROTECTION LA English DT Article DE Antimicrobial agents; Disinfectants; Non-0157 Shiga toxin-producing Escherichia coli; Organic acids; Susceptibility ID UNITED-STATES; ANTIBIOTIC-RESISTANCE; STAPHYLOCOCCUS-AUREUS; METHICILLIN-RESISTANT; CROSS-RESISTANCE; E. COLI; ANTISEPTICS; O157; SALMONELLA; INFECTIONS AB The disinfectant and antimicrobial susceptibility profiles of 138 non-O157 Shiga toxin producing Escherichia coli strains (STECs) from food animals and humans were determined. Antimicrobial resistance (AMR) was moderate (39.1% of strains) in response to 15 antimicrobial agents. Animal strains had a lower AMR prevalence (35.6%) than did human strains (43.9%) but a higher prevalence of the resistance profile GEN-KAN-TET. A decreasing prevalence of AMR was found among animal strains from serogroups O45 > O145 > O121 > O111 >O026 >O0103 and among human strains from serogroups O145 > O103 > O26 > O111 >O121 > O45. One animal strain from serogroups O121 and O145 and one human strain from serogroup O26 had extensive drug resistance. A high prevalence of AMR in animal O45 and O121 strains and no resistance or a low prevalence of resistance in human strains from these serogroups suggests a source other than food animals for human exposure to these strains. Among the 24 disinfectants evaluated, all strains were susceptible to triclosan. Animal strains had a higher prevalence of resistance to chlorhexidine than did human strains. Both animal and human strains had a similar low prevalence of low-level benzalkonium chloride resistance, and animal and human strains had similar susceptibility profiles for most other disinfectants. Benzyldimethylammonium chlorides and C10AC were the primary active components in disinfectants DC&R and P-128, respectively, against non-O157 STECs. A disinfectant FS512 MIC > 8 mu g/ml was more prevalent among animal O121 strains (61.5%) than among human O121 strains (25%), which may also suggest a source of human exposure to STEC O121 other than food animals. Bacterial inhibition was not dependent solely on pH but was correlated with the presence of dissociated organic acid species and some undissociated acids. C1 [Beier, Ross C.; Callaway, Todd R.; Andrews, Kathleen; Poole, Toni L.; Crippen, Tawni L.; Sheffield, Cynthia L.; Anderson, Robin C.; Nisbet, David J.] ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Franz, Eelco] Natl Inst Publ Hlth & Environm, Ctr Infect Dis Control Netherlands, Lab Zoonoses & Environm Microbiol, NL-3720 BA Bilthoven, Netherlands. [Bono, James L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Mandrell, Robert E.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Fratamico, Pina M.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Beier, RC (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. EM ross.beier@ars.usda.gov FU USDA, ARS FX The authors thank Max Heck (National Institute for Public Health and the Environment, Centre for Infectious Disease Control Netherlands [RIVM/IDS], Bilthoven, The Netherlands) for providing human strains and Amanda H. Vorpahl for technical support. This work was funded by the USDA, ARS. Mention of trade names, proprietary products, or specific equipment is solely for the purpose of providing specific information and does not constitute a guarantee, warranty, or endorsement by the USDA or by the U.S. Food and Drug Administration and does not imply its approval to the exclusion of other products that may be suitable. The views expressed in this article are those of the authors and do not necessarily reflect the official policy of the USDA or the U.S. Government. NR 67 TC 0 Z9 0 U1 16 U2 17 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2016 VL 79 IS 8 BP 1355 EP 1370 DI 10.4315/0362-028X.MP-15-600 PG 16 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA DT7GR UT WOS:000381655600008 PM 27497123 ER PT J AU Doll, KM Cermak, SC Kenar, JA Isbell, TA AF Doll, Kenneth M. Cermak, Steven C. Kenar, James A. Isbell, Terry A. TI Synthesis and Characterization of Estolide Esters Containing Epoxy and Cyclic Carbonate Groups SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Estolide; Carbonated vegetable oil; Cyclic carbonates; Supercritical carbon dioxide; Biobased lubricant ID RENEWABLE RAW-MATERIALS; EPOXIDIZED SOYBEAN OIL; TRIGLYCERIDE ESTOLIDES; PHYSICAL-PROPERTIES; DIALKYL CARBONATES; ORGANIC CARBONATES; FATTY-ACIDS; CASTOR OILS; LESQUERELLA; DIOXIDE AB The unsaturated sites in oleic 2-ethylhexyl estolide esters (containing 35 % monoenic fatty acids) were converted into epoxide and five-membered cyclic carbonate groups and the products characterized by Fourier transform infrared spectra (FTIR), H-1, and C-13 nuclear magnetic resonance (NMR) spectroscopies. Epoxidation of the alkene bonds was accomplished using performic acid generated in situ from formic acid and hydrogen peroxide. Greater than 90 % alkenes were converted into their corresponding epoxide groups as determined by oxirane values and the epoxide ring structure was confirmed by H-1 and C-13 NMR. The estolide ester epoxide material was subsequently reacted with supercritical carbon dioxide in the presence of tetrabutylammonium bromide catalyst to produce the corresponding estolide ester containing the cyclic carbonate group. The signals at 1,807 cm(-1) and delta 82 ppm in the FTIR and C-13-NMR spectra, respectively, confirmed the desired cyclic carbonate was produced. The carbonated estolide ester exhibited a dynamic viscosity, at 25 A degrees C, of 172 mPa center dot s as compared to 155 mPa center dot s for the estolide ester starting material. The estolide ester structure of these new derivatives was shown to be consistent throughout their synthesis. C1 [Doll, Kenneth M.; Cermak, Steven C.; Isbell, Terry A.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Kenar, James A.] ARS, Funct Foods Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. RP Doll, KM (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Kenneth.Doll@ars.usda.gov NR 36 TC 0 Z9 0 U1 11 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD AUG PY 2016 VL 93 IS 8 BP 1149 EP 1155 DI 10.1007/s11746-016-2857-y PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA DS0GT UT WOS:000380274200014 ER PT J AU Cajka, T Davis, R Austin, KJ Newman, JW German, JB Fiehn, O Smilowitz, JT AF Cajka, Tomas Davis, Ryan Austin, Kathryn J. Newman, John W. German, J. Bruce Fiehn, Oliver Smilowitz, Jennifer T. TI Using a lipidomics approach for nutritional phenotyping in response to a test meal containing gamma-linolenic acid SO METABOLOMICS LA English DT Article DE Dihomo-gamma-linolenic acid; Gammalinolenic acid; Lipidomics; Liquid chromatography-mass spectrometry; Phenotyping; SWATH ID POLYUNSATURATED FATTY-ACIDS; MASS-SPECTROMETRY; GENE-CLUSTER; EICOSAPENTAENOIC ACID; ARACHIDONIC-ACID; METABOLISM; BIOSYNTHESIS; LIPIDS; SUPPLEMENTATION; PHOSPHOLIPIDS AB Introduction Plasma fatty acids are derived from preformed sources in the diet and de novo synthesis through the action of desaturase and elongase enzymes. Objective This study was designed to examine the elongation of gamma-linolenic acid (GLA, 18:3n6) into dihomo-gamma-linolenic acid (DGLA, 20:3n6) over an 8-h period using both targeted gas chromatography-flame ionization detection and untargeted liquid chromatography-mass spectrometry-based lipidomics utilizing the sequential window acquisition of all theoretical fragmention spectra (SWATH). Methods In a single blind, placebo-controlled, crossover design, seven healthy subjects consumed a test meal that consisted of GLA fat (borage oil) or a control fat (a mixture of corn, safflower, sunflower and extra-virgin light olive oils) on three separate test days for each test meal. Results Total plasma fatty acid concentrations and 366 unique lipid species were measured at 0, 2, 4, 6 and 8 h in response to the test meals. Mean plasma 18:3n6 was 7-fold higher to the GLA challenge compared with baseline and the control meal. By 8 h, mean plasma 20:3n6 was significantly higher in response to the GLA test meal than baseline and the control group. Five of the seven subjects were "responders'' in converting GLA into DGLA, but two subjects did not show this conversion. The conversion was independent of physical activity level. Conclusion Using polyunsaturated fatty acid metabolism as an example, this study demonstrates inter-individual differences in enzymatic capacities to inform exact nutritional and metabolic phenotyping that could be used for precision medicine. C1 [Cajka, Tomas; Newman, John W.; Fiehn, Oliver] Univ Calif Davis, NIH, West Coast Metabol Ctr, Davis, CA 95616 USA. [Davis, Ryan] Lipomics, 3410 Ind Blvd,Suite 103, West Sacramento, CA USA. [Austin, Kathryn J.; German, J. Bruce; Smilowitz, Jennifer T.] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. [Austin, Kathryn J.; German, J. Bruce; Smilowitz, Jennifer T.] Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Newman, John W.] USDA ARS, Obes & Metab Res Unit, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Fiehn, Oliver] King Abdulaziz Univ, Dept Biochem, Fac Sci, POB 80203, Jeddah 21589, Saudi Arabia. RP Smilowitz, JT (reprint author), Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA.; Smilowitz, JT (reprint author), Univ Calif Davis, Foods Hlth Inst, Davis, CA 95616 USA. EM jensm@ucdavis.edu RI Cajka, Tomas/A-8914-2008 OI Cajka, Tomas/0000-0002-9728-3355 FU National Institute of Environmental Health Sciences (NIEHS) [P42ES004699]; NIEHS [R01 ES002710]; NIEHS Superfund Research Program [P42 ES011269]; CHARGE [P01 ES11269]; West Coast Metabolomics Center (NIH) [U24 DK097154]; NIH [1S10RR031630-01, P20 HL113452]; USDA Agricultural Research Service Projects [5306-51000-016-00D, 5306-51000-019-00D, 2032-51530-022-00D] FX This project was made possible in part by support from the National Institute of Environmental Health Sciences (NIEHS) (P42ES004699), NIEHS R01 ES002710, NIEHS Superfund Research Program P42 ES011269; the CHARGE study (P01 ES11269); West Coast Metabolomics Center (NIH U24 DK097154) and instrument support (NIH 1S10RR031630-01), cardiovascular research (NIH P20 HL113452) and USDA Agricultural Research Service Projects 5306-51000-016-00D, 5306-51000-019-00D and 2032-51530-022-00D. We would like to thank Dr. Vincent Ziboh in loving memory for his inspiration and insight for this project. We would like to thank the staff at the USDA, ARS, Western Human Nutrition Research Center, Davis, CA, for clinical, laboratory and kitchen support. The USDA is an equal opportunity provider and employer. We thank the study participants for their time efforts to commit to the study procedures. NR 43 TC 0 Z9 0 U1 7 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD AUG PY 2016 VL 12 IS 8 AR UNSP 127 DI 10.1007/s11306-016-1075-9 PG 16 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DS9DO UT WOS:000381083100001 ER PT J AU Berry, ED Wells, JE AF Berry, Elaine D. Wells, James E. TI Reducing Foodborne Pathogen Persistence and Transmission in Animal Production Environments: Challenges and Opportunities SO MICROBIOLOGY SPECTRUM LA English DT Article ID ESCHERICHIA-COLI O157-H7; ENTERICA SEROVAR TYPHIMURIUM; AVIUM SUBSP PARATUBERCULOSIS; BEEF PROCESSING PLANTS; WET DISTILLERS GRAINS; EXPERIMENTAL CHLORATE PRODUCT; PREHARVEST FOOD SAFETY; PROTEINS-BASED VACCINE; ILEOCECAL LYMPH-NODES; IN-GROUND BEEF AB Preharvest strategies to reduce zoonotic pathogens in food animals are important components of the farm-to-table food safety continuum. The problem is complex; there are multiple pathogens of concern, multiple animal species under different production and management systems, and a variety of sources of pathogens, including other livestock and domestic animals, wild animals and birds, insects, water, and feed. Preharvest food safety research has identified a number of intervention strategies, including probiotics, direct-fed microbials, competitive exclusion cultures, vaccines, and bacteriophages, in addition to factors that can impact pathogens on-farm, such as seasonality, production systems, diet, and dietary additives. Moreover, this work has revealed both challenges and opportunities for reducing pathogens in food animals. Animals that shed high levels of pathogens and predominant pathogen strains that exhibit long-term persistence appear to play significant roles in maintaining the prevalence of pathogens in animals and their production environment. Continued investigation and advancements in sequencing and other technologies are expected to reveal the mechanisms that result in super-shedding and persistence, in addition to increasing the prospects for selection of pathogen-resistant food animals and understanding of the microbial ecology of the gastrointestinal tract with regard to zoonotic pathogen colonization. It is likely that this continued research will reveal other challenges, which may further indicate potential targets or critical control points for pathogen reduction in livestock. Additional benefits of the preharvest reduction of pathogens in food animals are the reduction of produce, water, and environmental contamination, and thereby lower risk for human illnesses linked to these sources. C1 [Berry, Elaine D.; Wells, James E.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Berry, ED (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Elaine.Berry@ars.usda.gov NR 225 TC 0 Z9 0 U1 15 U2 16 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA EI 2165-0497 J9 MICROBIOL SPECTR JI Microbiol. Spectr. PD AUG PY 2016 VL 4 IS 4 AR UNSP PFS-0006-2014 DI 10.1128/microbiolspec.PFS-0006-2014 PG 18 WC Microbiology SC Microbiology GA DT9GI UT WOS:000381804000038 ER PT J AU Kingsley, DH AF Kingsley, David H. TI Emerging Foodborne and Agriculture-Related Viruses SO MICROBIOLOGY SPECTRUM LA English DT Article ID HEPATITIS-E-VIRUS; RESPIRATORY SYNDROME CORONAVIRUS; PUBLIC-HEALTH SIGNIFICANCE; BOVINE LEUKEMIA-VIRUS; TORQUE-TENO-VIRUS; UNITED-STATES; HUMAN ADENOVIRUS; HUMAN BOCAVIRUS; DILATED CARDIOMYOPATHY; CEREBROSPINAL-FLUID AB Viruses rapidly evolve and can emerge in unpredictable ways. Transmission pathways by which foodborne viruses may enter human populations and evolutionary mechanisms by which viruses can become virulent are discussed in this chapter. A majority of viruses emerge from zoonotic animal reservoirs, often by adapting and infecting intermediate hosts, such as domestic animals and livestock. Viruses that are known foodborne threats include hepatitis E virus, tick-borne encephalitis virus, enteroviruses, adenovirus, and astroviruses, among others. Viruses may potentially evolve and emerge as a result of modern agricultural practices which can concentrate livestock and bring them into contact with wild animals. Examples of viruses that have emerged in this manner are influenza, coronaviruses such as severe acute respiratory syndrome and Middle East respiratory syndrome, and the Nipah virus. The role of bats, bush meat, rodents, pigs, cattle, and poultry as reservoirs from which infectious pathogenic viruses emerge are discussed. C1 [Kingsley, David H.] Delaware State Univ, USDA, ARS, Food Safety & Intervent Res Unit, Dover, DE 19901 USA. RP Kingsley, DH (reprint author), Delaware State Univ, USDA, ARS, Food Safety & Intervent Res Unit, Dover, DE 19901 USA. EM David.Kingsley@ars.usda.gov NR 148 TC 0 Z9 0 U1 6 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA EI 2165-0497 J9 MICROBIOL SPECTR JI Microbiol. Spectr. PD AUG PY 2016 VL 4 IS 4 AR UNSP PFS-0007-2014 DI 10.1128/microbiolspec.PFS-0007-2014 PG 15 WC Microbiology SC Microbiology GA DT9GI UT WOS:000381804000039 ER PT J AU Sharma, M Reynnells, R AF Sharma, Manan Reynnells, Russell TI Importance of Soil Amendments: Survival of Bacterial Pathogens in Manure and Compost Used as Organic Fertilizers SO MICROBIOLOGY SPECTRUM LA English DT Article ID ESCHERICHIA-COLI O157H7; ANTIBIOTIC-RESISTANT BACTERIA; AMENDED SOIL; ENTERIC MICROORGANISMS; SALMONELLA-ENTERICA; ZOONOTIC PATHOGENS; FINISHED COMPOST; IRRIGATION WATER; FEEDLOT CATTLE; UNITED-STATES AB Biological soil amendments (BSAs) such as manure and compost are frequently used as organic fertilizers to improve the physical and chemical properties of soils. However, BSAs have been known to be a reservoir for enteric bacterial pathogens such as enterohemorrhagic Escherichia coli (EHEC), Salmonella spp., and Listeria spp. There are numerous mechanisms by which manure may transfer pathogens to growing fruits and vegetables, and several outbreaks of infections have been linked to manure-related contamination of leafy greens. In the United States several commodity-specific guidelines and current and proposed federal rules exist to provide guidance on the application of BSAs as fertilizers to soils, some of which require an interval between the application of manure to soils and the harvest of fruits and vegetables. This review examines the survival, persistence, and regrowth/resuscitation of bacterial pathogens in manure, biosolids, and composts. Moisture, along with climate and the physicochemical properties of soil, manure, or compost, plays a significant role in the ability of pathogens to persist and resuscitate in amended soils. Adaptation of enteric bacterial pathogens to the nonhost environment of soils may also extend their persistence in manure-or compost-amended soils. The presence of antibiotic-resistance genes in soils may also be increased by manure application. Overall, BSAs applied as fertilizers to soils can support the survival and regrowth of pathogens. BSAs should be handled and applied in a manner that reduces the prevalence of pathogens in soils and the likelihood of transfer of food-borne pathogens to fruits and vegetables. This review will focus on two BSAs-raw manure and composted manure (and other feedstocks)-and predominantly on the survival of enteric bacterial pathogens in BSAs as applied to soils as organic fertilizers. C1 [Sharma, Manan] ARS, USDA, Beltsville Area Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Reynnells, Russell] Univ Maryland Eastern Shore, Dept Agr Food & Resource Sci, Princess Anne, MD 21853 USA. RP Sharma, M (reprint author), ARS, USDA, Beltsville Area Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM manan.sharma@ars.usda.gov NR 67 TC 0 Z9 0 U1 21 U2 27 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA EI 2165-0497 J9 MICROBIOL SPECTR JI Microbiol. Spectr. PD AUG PY 2016 VL 4 IS 4 AR UNSP PFS-0010-2015 DI 10.1128/microbiolspec.PFS-0010-2015 PG 13 WC Microbiology SC Microbiology GA DT9GI UT WOS:000381804000041 ER PT J AU Early, R Bradley, BA Dukes, JS Lawler, JJ Olden, JD Blumenthal, DM Gonzalez, P Grosholz, ED Ibanez, I Miller, LP Sorte, CJB Tatem, AJ AF Early, Regan Bradley, Bethany A. Dukes, Jeffrey S. Lawler, Joshua J. Olden, Julian D. Blumenthal, Dana M. Gonzalez, Patrick Grosholz, Edwin D. Ibanez, Ines Miller, Luke P. Sorte, Cascade J. B. Tatem, Andrew J. TI Global threats from invasive alien species in the twenty-first century and national response capacities SO NATURE COMMUNICATIONS LA English DT Article ID PLANT INVASIONS; UNITED-STATES; BIOLOGICAL INVASIONS; CLIMATE-CHANGE; GREAT-BRITAIN; TRADE; BIODIVERSITY; RISK; INTRODUCTIONS; RICHNESS AB Invasive alien species (IAS) threaten human livelihoods and biodiversity globally. Increasing globalization facilitates IAS arrival, and environmental changes, including climate change, facilitate IAS establishment. Here we provide the first global, spatial analysis of the terrestrial threat from IAS in light of twenty-first century globalization and environmental change, and evaluate national capacities to prevent and manage species invasions. We find that one-sixth of the global land surface is highly vulnerable to invasion, including substantial areas in developing economies and biodiversity hotspots. The dominant invasion vectors differ between high-income countries (imports, particularly of plants and pets) and low-income countries (air travel). Uniting data on the causes of introduction and establishment can improve early-warning and eradication schemes. Most countries have limited capacity to act against invasions. In particular, we reveal a clear need for proactive invasion strategies in areas with high poverty levels, high biodiversity and low historical levels of invasion. C1 [Early, Regan] Univ Exeter, Ctr Ecol & Conservat, Penryn Campus, Penryn TR10 9FE, Cornwall, England. [Bradley, Bethany A.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Lawler, Joshua J.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Olden, Julian D.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Blumenthal, Dana M.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO USA. [Gonzalez, Patrick] Natl Pk Serv, Nat Resource Stewardship & Sci, Berkeley, CA 94720 USA. [Gonzalez, Patrick] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Grosholz, Edwin D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Ibanez, Ines] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. [Miller, Luke P.] Stanford Univ, Hopkins Marine Stn, Pacific Grove, CA 93950 USA. [Sorte, Cascade J. B.] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92697 USA. [Tatem, Andrew J.] Univ Southampton, Dept Geog & Environm, Southampton SO17 1BJ, Hants, England. [Tatem, Andrew J.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA. [Tatem, Andrew J.] Flowminder Fdn, SE-11355 Stockholm, Sweden. RP Early, R (reprint author), Univ Exeter, Ctr Ecol & Conservat, Penryn Campus, Penryn TR10 9FE, Cornwall, England. EM r.early@exeter.ac.uk RI Gonzalez, Patrick/B-9479-2013 OI Gonzalez, Patrick/0000-0002-7105-0561 FU University of California, Santa Barbara; State of California; National Center for Ecological Analysis and Synthesis - National Science Foundation [EF-0553768]; NERC GW4+ DTP PhD 'Unexplained limits on species distributions. What do they mean for conservation?' FX We thank Ricardo Dobrovolski, Andy Nelson, Ben Halpern, Catarina Meireles, Sarah Ayton and Ryan Bird Rafalski for supplying or collating data, and Rashid Al Badwawi, Olga Dmitrieva, Manar Maraqa and Nazmi Sellami for help with translation. The analysis was conducted as part of the Climate Change & Invasive Species Working Group supported by the National Center for Ecological Analysis and Synthesis, a Center funded by the National Science Foundation (grant #EF-0553768), the University of California, Santa Barbara, and the State of California. The work was supported by the NERC GW4+ DTP PhD 'Unexplained limits on species distributions. What do they mean for conservation?' NR 76 TC 1 Z9 1 U1 71 U2 81 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD AUG PY 2016 VL 7 AR 12485 DI 10.1038/ncomms12485 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DU1DG UT WOS:000381947800001 PM 27549569 ER PT J AU Somo, M Pirseyedi, SM Cai, XW Poudel, RS Chao, SM Marais, F AF Somo, Mohamed Pirseyedi, Seyed Mostafa Cai, Xiwen Poudel, Roshan Sharma Chao, Shiaoman Marais, Francois TI Mapping of Lr56 translocation recombinants in wheat SO PLANT BREEDING LA English DT Article DE gene transfer; leaf rust resistance; breeding; wide hybridization ID RUST RESISTANCE; LEAF RUST; AFLP; STEM AB In an attempt to transfer the Lr56/Yr38 resistance loci from Aegilops sharonensis to wheat, a 6A-6S(sh) chromosome translocation was produced. It involves essentially the entire chromosome 6S(sh) with a small terminal segment of 6AL. Induced homoeologous recombination of the translocated chromosome with 6A produced numerous recombinants including three recombined chromosomes carrying Lr56 that could not be precisely mapped for lack of suitable markers. This study aimed to determine the chromosomal locations of the translocation breakpoints in these three recombinants using various DNA markers as well as physical and genetic mapping. The three recombinants Lr56-39, -157 and -175 carry small segments of Ae.sharonensis chromatin distally to the Xgpw4329 and IWA5416 loci near the 6AS telomere. The Ae.sharonensis chromatin that remains in each line includes a homoeolocus of the wheat marker locus Xdupw217 (on 6BS) and its characteristic amplification product can be used as a dominant marker for the presence of Lr56. Of the three recombined chromosomes, Lr56-157 retained the least alien chromatin and appears to be the best candidate for use in wheat breeding. C1 [Somo, Mohamed; Pirseyedi, Seyed Mostafa; Cai, Xiwen; Marais, Francois] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Poudel, Roshan Sharma] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Chao, Shiaoman] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. RP Marais, F (reprint author), North Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. EM Gideon.marais@ndsu.edu FU North Dakota Wheat Commission; Minnesota Wheat Research and Promotion Council FX We thank the North Dakota Wheat Commission and Minnesota Wheat Research and Promotion Council for financial support. We also wish to thank Mary Osenga (US Department of Agriculture, Agricultural Research Service Biosciences Research Laboratory, Fargo, ND 58102) for technical assistance. NR 17 TC 0 Z9 0 U1 4 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD AUG PY 2016 VL 135 IS 4 BP 413 EP 419 DI 10.1111/pbr.12383 PG 7 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DS7KF UT WOS:000380961700002 ER PT J AU Abdel-Ghani, AH Hu, SL Chen, YS Brenner, EA Kumar, B Blanco, M Lubberstedt, T AF Abdel-Ghani, Adel H. Hu, Songlin Chen, Yongsheng Brenner, Everton A. Kumar, Bharath Blanco, Michael Lubberstedt, Thomas TI Genetic architecture of plant height in maize phenotype-selected introgression families SO PLANT BREEDING LA English DT Article DE Zea mays L; plant height; phenotypic-selected introgression families; donor chromosomal segments; genetic variation ID QUANTITATIVE TRAIT LOCI; NEAR-ISOGENIC LINES; MARKER-ASSISTED SELECTION; BACKCROSS QTL ANALYSIS; ORYZA-SATIVA L.; DROUGHT TOLERANCE; GRAIN-YIELD; LYCOPERSICON-ESCULENTUM; SUBSTITUTION LINES; DIFFERENT TESTERS AB This study aimed at developing, characterizing and evaluating two maize phenotypic-selected introgression libraries for a collection of dominant plant height (PHT)-increasing alleles by introgressing donor chromosome segments (DCS) from Germplasm Enhancement of Maize (GEM) accessions into elite inbred lines: PHB47 and PHZ51. Different backcross generations (BC1-BC4) were developed and the tallest 23 phenotype-selected introgression families (PIFs) from each introgression library (PHB47 or PHZ51) were selected for single nucleotide polymorphism genotyping to localize DCS underlying PHT. The result shows that most PIFs carrying DCS were significantly (=0.01) taller than the respective recurrent parent. In addition, they contained larger donor genome proportions than expected in the absence of selection or random mating across all BC generations. The DCS were distributed over the whole genome, indicating a complex genetic nature underlying PHT. We conclude that our PIFs are enriched for favourable PHT-increasing alleles. These two libraries offer opportunities for future PHT gene isolation and allele characterization and for breeding purposes, such as novel cultivars for biofuel production. C1 [Abdel-Ghani, Adel H.] Mutah Univ, Dept Plant Prod, Fac Agr, POB 7, Al Karak BOX7, Jordan. [Hu, Songlin; Chen, Yongsheng; Brenner, Everton A.; Kumar, Bharath; Lubberstedt, Thomas] Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50011 USA. [Blanco, Michael] Iowa State Univ, USDA ARS, Plant Introduct Res Unit, Dept Agron, Ames, IA 50011 USA. RP Lubberstedt, T (reprint author), Iowa State Univ, Dept Agron, Agron Hall, Ames, IA 50011 USA. EM thomasl@iastate.edu FU China Scholarship Council FX This study was prepared while Dr. Adel Abdel-Ghani was a visiting Fulbright Postdoctoral Fellow and during the sabbatical leave granted to Dr. Adel Abdel-Ghani from Mu'tah University, Jordan, during the academic year 2011-2012 at Iowa State University (ISU), Ames, USA. We would like to thank the RF Baker Center for Plant Breeding and China Scholarship Council for Songlin's funding. NR 66 TC 0 Z9 0 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD AUG PY 2016 VL 135 IS 4 BP 429 EP 438 DI 10.1111/pbr.12387 PG 10 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA DS7KF UT WOS:000380961700004 ER PT J AU Yang, TH Fang, LL Rimando, AM Sobolev, V Mockaitis, K Medina-Bolivar, F AF Yang, Tianhong Fang, Lingling Rimando, Agnes M. Sobolev, Victor Mockaitis, Keithanne Medina-Bolivar, Fabricio TI A Stilbenoid-Specific Prenyltransferase Utilizes Dimethylallyl Pyrophosphate from the Plastidic Terpenoid Pathway SO PLANT PHYSIOLOGY LA English DT Article ID PEANUT ARACHIS-HYPOGAEA; HAIRY ROOT CULTURES; CELL-SUSPENSION CULTURES; PRENYLATED STILBENES; SOPHORA-FLAVESCENS; METHYL JASMONATE; RESVERATROL; BIOSYNTHESIS; PHYTOALEXINS; CYCLODEXTRIN AB Prenylated stilbenoids synthesized in some legumes exhibit plant pathogen defense properties and pharmacological activities with potential benefits to human health. Despite their importance, the biosynthetic pathways of these compounds remain to be elucidated. Peanut (Arachis hypogaea) hairy root cultures produce a diverse array of prenylated stilbenoids upon treatment with elicitors. Using metabolic inhibitors of the plastidic and cytosolic isoprenoid biosynthetic pathways, we demonstrated that the prenyl moiety on the prenylated stilbenoids derives from a plastidic pathway. We further characterized, to our knowledge for the first time, a membrane-bound stilbenoid-specific prenyltransferase activity from the microsomal fraction of peanut hairy roots. This microsomal fraction-derived resveratrol 4-dimethylallyl transferase utilizes 3,3-dimethylallyl pyrophosphate as a prenyl donor and prenylates resveratrol to form arachidin-2. It also prenylates pinosylvin to chiricanine A and piceatannol to arachidin-5, a prenylated stilbenoid identified, to our knowledge, for the first time in this study. This prenyltransferase exhibits strict substrate specificity for stilbenoids and does not prenylate flavanone, flavone, or isoflavone backbones, even though it shares several common features with flavonoid-specific prenyltransferases. C1 [Yang, Tianhong; Fang, Lingling; Medina-Bolivar, Fabricio] Arkansas State Univ, Arkansas Biosci Inst, Jonesboro, AR 72467 USA. [Yang, Tianhong] Arkansas State Univ, Mol Biosci Grad Program, Jonesboro, AR 72467 USA. [Fang, Lingling; Medina-Bolivar, Fabricio] Arkansas State Univ, Dept Biol Sci, Jonesboro, AR 72467 USA. [Rimando, Agnes M.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Sobolev, Victor] Natl Peanut Res Lab, Dawson, GA 39842 USA. [Mockaitis, Keithanne] Indiana Univ, Pervas Technol Inst, Bloomington, IN 47408 USA. [Mockaitis, Keithanne] Indiana Univ, Dept Biol, Bloomington, IN 47408 USA. RP Medina-Bolivar, F (reprint author), Arkansas State Univ, Arkansas Biosci Inst, Jonesboro, AR 72467 USA.; Medina-Bolivar, F (reprint author), Arkansas State Univ, Dept Biol Sci, Jonesboro, AR 72467 USA. EM fmedinabolivar@astate.edu FU U.S. Department of Agriculture-NIFA [2014-67014-21701]; National Science Foundation-EPSCoR (Center for Plant-Powered Production) [EPS 0701890]; Arkansas ASSET Initiative; Arkansas Science and Technology Authority; Arkansas Biosciences Institute FX This work was supported by the U.S. Department of Agriculture-NIFA (grant no. 2014-67014-21701), the National Science Foundation-EPSCoR (grant no. EPS 0701890, Center for Plant-Powered Production), the Arkansas ASSET Initiative, the Arkansas Science and Technology Authority, and the Arkansas Biosciences Institute. NR 45 TC 0 Z9 0 U1 5 U2 10 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD AUG PY 2016 VL 171 IS 4 BP 2483 EP 2498 DI 10.1104/pp.16.00610 PG 16 WC Plant Sciences SC Plant Sciences GA DT2IJ UT WOS:000381303700018 PM 27356974 ER PT J AU Boydston, RA Williams, MM AF Boydston, R. A. Williams, M. M., II TI Sweet corn hybrid tolerance to weed competition under three weed management levels SO RENEWABLE AGRICULTURE AND FOOD SYSTEMS LA English DT Article DE crop competition; crop tolerance; cultivation; relative yield; sweet corn; Zea mays L; weed competition; weed management; weed suppressive ability ID MILLET PANICUM-MILIACEUM; SEEDING RATE; ABILITY; L. AB Nearly all commercial sweet corn fields contain weeds that escaped management and, therefore, sweet corn often suffers yield losses due to weed competition. For this reason, field trials were conducted from 2009 to 2011 near Prosser, WA and Urbana, IL to evaluate the responses of weeds and four sweet corn hybrids to three levels of weed management; weed free, high intensity cultivation (HC), and low intensity cultivation (LC). Weed management level had the greatest impact on early season weed densities and HC reduced final weed biomass more than LC in 2 of 4 site-years. Two taller sweet corn hybrids with greater leaf area suppressed final weed biomass more than two shorter hybrids with less leaf area in 3 of 4 site-years. When grown with less intense weed management that resulted in more weeds, taller sweet corn hybrids with greater leaf area maintained yields better than shorter, less competitive sweet corn hybrids. Utilizing hybrids with greater tolerance to weeds and greater ability to suppress weeds could be a valuable component of an integrated weed management system. C1 Agronomist, Agricultural Research Service, United States Department of Agriculture, Prosser, WA 99350 USA. Ecologist, Agricultural Research Service, United States Department of Agriculture, Urbana, IL 61801 USA. [Boydston, R. A.] ARS, USDA, 24106 N Bun Rd, Prosser, WA 99350 USA. [Williams, M. M., II] ARS, USDA, 1102 S Goodwin Ave, Urbana, IL 61801 USA. RP Boydston, RA (reprint author), ARS, USDA, 24106 N Bun Rd, Prosser, WA 99350 USA. EM rick.boydston@ars.usda.gov NR 14 TC 0 Z9 0 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1742-1705 EI 1742-1713 J9 RENEW AGR FOOD SYST JI Renew. Agr. Food Syst. PD AUG PY 2016 VL 31 IS 4 BP 281 EP 287 DI 10.1017/S1742170515000204 PG 7 WC Agriculture, Multidisciplinary SC Agriculture GA DS9FU UT WOS:000381089100001 ER PT J AU Yu, Y Chen, CX Gmitter, FG AF Yu, Yuan Chen, Chunxian Gmitter, Frederick G., Jr. TI QTL mapping of mandarin (Citrus reticulata) fruit characters using high-throughput SNP markers SO TREE GENETICS & GENOMES LA English DT Article DE Mapping; Fruit quality; Mandarin; Breeding ID QUANTITATIVE TRAIT LOCI; P-HYDROXYPHENYLPYRUVATE DIOXYGENASE; INTERGENERIC BC1 PROGENY; VIRUS-RESISTANCE GENE; SINENSIS L. OSBECK; RFLP LINKAGE MAPS; MOLECULAR MARKERS; PONCIRUS-TRIFOLIATA; CAROTENOID CONTENT; NONSALINE ENVIRONMENTS AB Seedlessness, flavor, and color are top priorities for mandarin (Citrus reticulata Blanco) cultivar improvement. Given long juvenility, large tree size, and high breeding cost, marker-assisted selection (MAS) may be an expeditious and economical approach to these challenges. The objectives of this study were to construct high-density mandarin genetic maps and to identify single nucleotide polymorphism (SNP) markers associated with fruit quality traits. Two parental genetic maps were constructed from an F-1 population derived from 'Fortune' x 'Murcott', two mandarin cultivars with distinct fruit characters, using a 1536-SNP Illumina GoldenGate assay. The map for 'Fortune' (FOR) consisted of 189 SNPs spanning 681.07 cM and for 'Murcott' (MUR) consisted of 106 SNPs spanning 395.25 cM. Alignment of the SNP sequences to the Clementine (Citrus clementina) genome showed highly conserved synteny between the genetic maps and the genome. A total of 48 fruit quality quantitative trait loci (QTLs) were identified, and ten of them stable over two or more samplings were considered as major QTLs. A cluster of QTLs for flavedo color space values L, a, b, and a/b and juice color space values a and a/b were detected in a single genomic region on linkage group 4. Two carotenoid biosynthetic pathway genes, pds1 and ccd4, were found within this QTL interval. Several SNPs were potentially useful in MAS for these fruit characteristics. QTLs were validated in 13 citrus selections, which may be useful in further validation and tentative MAS in mandarin fruit quality improvement. C1 [Yu, Yuan; Gmitter, Frederick G., Jr.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Chen, Chunxian] ARS, SE Fruit & Tree Nut Res Lab, USDA, Byron, GA 31008 USA. RP Gmitter, FG (reprint author), Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. EM ymmzyz@ufl.edu; Chunxian.Chen@ars.usda.gov; fgmitter@ufl.edu FU New Varieties Development & Management Corporation; Citrus Research and Development Foundation; University of Florida Plant Molecular Breeding Initiative FX This work was partly funded by New Varieties Development & Management Corporation, Citrus Research and Development Foundation, and the University of Florida Plant Molecular Breeding Initiative. The authors thank Yanzi Zhang, Marjorie Wendell, Xu Wei, Qibin Yu, and Misty Holt for technical assistance, as well as Sanghamitra Das for help with editing and Harry Klee for critical reading of the manuscript. NR 81 TC 0 Z9 0 U1 17 U2 17 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD AUG PY 2016 VL 12 IS 4 AR 77 DI 10.1007/s11295-016-1034-7 PG 16 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA DS3KT UT WOS:000380682500015 ER PT J AU Calero-Bernal, R Perez-Martin, JE Reina, D Serrano, FJ Frontera, E Fuentes, I Dubey, JP AF Calero-Bernal, R. Perez-Martin, J. E. Reina, D. Serrano, F. J. Frontera, E. Fuentes, I. Dubey, J. P. TI Detection of Zoonotic Protozoa Toxoplasma gondii and Sarcocystis suihominis in Wild Boars from Spain SO ZOONOSES AND PUBLIC HEALTH LA English DT Article DE Wild Boar; Spain; prevalence; Toxoplasma gondii; Sarcocystis suihominis; Sarcocystis miescheriana ID SUS-SCROFA; PIGS; SEROPREVALENCE; PREVALENCE; SARCOSPORIDIA; INFECTIONS AB Food safety regulations require the control of the presence of protozoa in meats destined for human consumption. Wild boar (Sus scrofa) meat may constitute a source of zoonoses. A 23.8% (688/2881) seroprevalence of anti-Toxoplasma gondii antibodies and 72.2% (662/910) Sarcocystis sarcocysts prevalence were detected among wild boars hunted in Southwestern areas of Spain. Identity of Sarcocystis spp. was performed by RFLP-PCR and sequencing, detecting S. miescheriana (7/8) and the zoonotic S. suihominis (1/8). Risk assessment studies of these coccidian in meats destined to human consumption are needed. C1 [Calero-Bernal, R.; Perez-Martin, J. E.; Reina, D.; Serrano, F. J.; Frontera, E.] Univ Extremadura, Dept Anim Hlth, Fac Vet, Area Parasitol, Caceres, Spain. [Calero-Bernal, R.; Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Fuentes, I.] Inst Salud Carlos III, Parasitol Serv, Microbiol Natl Ctr, Madrid, Spain. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov FU INIA [FAU2006-00016]; Extremadura Regional Government [PRI08A102] FX The authors thank Natalia Calderon, Alejandro Tovar and Begona Bailo for technical assistance and sample collection. Study was funded by INIA (Project No. FAU2006-00016) and Extremadura Regional Government (Project No. PRI08A102). NR 30 TC 2 Z9 2 U1 8 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD AUG PY 2016 VL 63 IS 5 BP 346 EP 350 DI 10.1111/zph.12243 PG 5 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA DS7IR UT WOS:000380957300002 PM 26604045 ER PT J AU Bolzenius, JK Cushman, RA Perry, GA AF Bolzenius, Jennifer K. Cushman, Robert A. Perry, George A. TI Expression of Na+/H+ exchanger isoforms 1, 2, 3, and 4 in bovine endometrium and the influence of uterine pH at time of fixed-time AI of pregnancy success SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Beef cattle; FTAI protocols; Uterine pH; Pregnancy rate ID CAUDAL EPIDIDYMAL FLUID; HAMSTER SPERM ACROSOME; GUINEA-PIG SPERMATOZOA; INTRACELLULAR PH; ARTIFICIAL-INSEMINATION; QUIESCENCE FACTOR; STANDING ESTRUS; ESTROUS-CYCLE; BICARBONATE; MOTILITY AB Cows that exhibit estrus prior to fixed-time AI had increased sperm transport to the site of fertilization, and improved embryo quality on d 6 after insemination. Sperm transport is influenced by uterine pH, and research has reported that uterine pH decreased at onset of estrus, but must return to normal prior to ovulation. Therefore, the objectives of these studies were to investigate a possible mechanism for the regulation of uterine pH around the onset of estrus, and to determine if uterine pH at time of fixed-time AI influenced pregnancy success. In experiment 1, Angus-cross beef cows (n = 40 and 28 in rep. 1 and 2, respectively) were synchronized with the PG 6-day CIDR protocol (PGF(2)alpha on d -9, GnRH and insertion of a CIDR on d -6, and PGF(2)alpha. and CIDR removal on d 0). Cows were blocked by follicle size at time of CIDR removal, and uterine biopsies were collected at 0, 12, 24, 36, 48, 60 (Rep. 1), 72, 84, or 96 h (Rep2) after CIDR removal, and total cellular RNA was extracted from all biopsies. Estrus was monitored by the HeatWatch Estrous Detection System. In experiment 2, 223 postpartum beef cows in 2 herds were synchronized with a fixed-time AI protocol (herd 1: n = 97; CO-Synch plus CIDR protocol; herd 2: n = 126; Co-synch protocol). Uterine pH was determined at time of AI (n=80 and 63 for herd 1 and 2, respectively), and estrus was monitored by visual estrus detection with the aid of an ESTROTECT estrous detection patches, and pregnancy was determined by transrectal ultrasonography. In experiment 1, there was a significant (P < 0.01), quadratic relationship in expression of Na+/H+ exchanger isoforms 1, 2, and 3 among animals that exhibited estrus, with expression greatest at time of CIDR removal, decreasing to the onset of estrus, and then increasing again following the onset of estrus. Among cows that did not exhibit estrus, the preceding relationship did not exist (P>0.46). In experiment 2, cows that had initiated estrus prior to fixed-time AI had decreased (P=0.01) uterine pH compared to cows that did not initiate estrus (6.78 +/- 0.03 and 6.89 +/- 0.03, respectively), and uterine pH at AI had an approximately linear effect on pregnancy success within the observed pH range. Furthermore, cows that initiated estrus prior to AI had increased (P = 0.05) pregnancy success (52% vs. 38%) compared to cows that had not initiated estrus. In summary, expression of Na+/H+ exchanger isoforms 1, 2, and 3 decreased after CIDR removal among cows that exhibited estrus, but did not change among cows that did not exhibit estrus. Additionally, as uterine pH decreased pregnancy success tended to increase (P=0.076, logistics regression). Thus, Na+/H+ exchanger isoforms 1, 2, and 3 appear to be key regulators of uterine pH around the onset of estrus, and this change in uterine pH is critical for pregnancy success. Expression of Na+/H+ exchanger isoforms 1, 2, and 3 decreased after CIDR removal among cows that exhibited estrus, but did not change among cows that did not exhibit estrus, and as uterine pH decreased, pregnancy success tended to increase. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bolzenius, Jennifer K.; Perry, George A.] S Dakota State Univ, Dept Anim Sci, Box 2170,ASC 214, Brookings, SD 57007 USA. [Cushman, Robert A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Perry, GA (reprint author), S Dakota State Univ, Dept Anim Sci, Box 2170,ASC 214, Brookings, SD 57007 USA. EM George.Perry@sdstate.edu OI Perry, George/0000-0002-0102-9046 FU South Dakota Agriculture Experiment Station Hatch funds FX Mention of a proprietary product does not constitute a guarantee or warranty of the product by South Dakota AES, or the authors, and does not imply its approval to the exclusion of other products that may also be suitable. This project was funded by the South Dakota Agriculture Experiment Station Hatch funds. NR 38 TC 1 Z9 1 U1 2 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 EI 1873-2232 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD AUG PY 2016 VL 171 BP 98 EP 107 DI 10.1016/j.anireprosci.2016.06.004 PG 10 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA DS2OP UT WOS:000380623700013 PM 27338797 ER PT J AU Daou, M Piumi, F Cullen, D Record, E Faulds, CB AF Daou, Marianne Piumi, Francois Cullen, Daniel Record, Eric Faulds, Craig B. TI Heterologous Production and Characterization of Two Glyoxal Oxidases from Pycnoporus cinnabarinus SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID PHANEROCHAETE-CHRYSOSPORIUM; GALACTOSE-OXIDASE; MANGANESE PEROXIDASE; ASPERGILLUS-NIGER; EXPRESSION; ENZYMES; GENE; OXIDATION; SYSTEMS; ACID AB The genome of the white rot fungus Pycnoporus cinnabarinus includes a large number of genes encoding enzymes implicated in lignin degradation. Among these, three genes are predicted to encode glyoxal oxidase, an enzyme previously isolated from Phanerochaete chrysosporium. The glyoxal oxidase of P. chrysosporium is physiologically coupled to lignin-oxidizing peroxidases via generation of extracellular H2O2 and utilizes an array of aldehydes and alpha-hydroxycarbonyls as the substrates. Two of the predicted glyoxal oxidases of P. cinnabarinus, GLOX1 (PciGLOX1) and GLOX2 (PciGLOX2), were heterologously produced in Aspergillus niger strain D15#26 (pyrG negative) and purified using immobilized metal ion affinity chromatography, yielding 59 and 5 mg of protein for PciGLOX1 and PciGLOX2, respectively. Both proteins were approximately 60 kDa in size and N-glycosylated. The optimum temperature for the activity of these enzymes was 50 degrees C, and the optimum pH was 6. The enzymes retained most of their activity after incubation at 50 degrees C for 4 h. The highest relative activity and the highest catalytic efficiency of both enzymes occurred with glyoxylic acid as the substrate. The two P. cinnabarinus enzymes generally exhibited similar substrate preferences, but PciGLOX2 showed a broader substrate specificity and was significantly more active on 3-phenylpropionaldehyde. IMPORTANCE This study addresses the poorly understood role of how fungal peroxidases obtain an in situ supply of hydrogen peroxide to enable them to oxidize a variety of organic and inorganic compounds. This cooperative activity is intrinsic in the living organism to control the amount of toxic H2O2 in its environment, thus providing a feed-on-demand scenario, and can be used biotechnologically to supply a cheap source of peroxide for the peroxidase reaction. The secretion of multiple glyoxal oxidases by filamentous fungi as part of a lignocellulolytic mechanism suggests a controlled system, especially as these enzymes utilize fungal metabolites as the substrates. Two glyoxal oxidases have been isolated and characterized to date, and the differentiation of the substrate specificity of the two enzymes produced by Pycnoporus cinnabarinus illustrates the alternative mechanisms existing in a single fungus, together with the utilization of these enzymes to prepare platform chemicals for industry. C1 [Daou, Marianne; Piumi, Francois; Record, Eric; Faulds, Craig B.] Aix Marseille Univ, INRA, BBF, Marseille, France. [Cullen, Daniel] USDA, Forest Prod Lab, Madison, WI 53705 USA. [Piumi, Francois] INRA, Unite Biol Dev & Reprod, Jouy En Josas, France. RP Faulds, CB (reprint author), Aix Marseille Univ, INRA, BBF, Marseille, France. EM craig.faulds@univ-amu.fr FU European Commission (EC) [KBBE-2013-7-613549] FX This work, including the efforts of Marianne Daou, Francois Piumi, Eric Record, and Craig Faulds, was funded by European Commission (EC) (KBBE-2013-7-613549). NR 32 TC 0 Z9 0 U1 10 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD AUG PY 2016 VL 82 IS 16 BP 4867 EP 4875 DI 10.1128/AEM.00304-16 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DS2EG UT WOS:000380550900003 PM 27260365 ER PT J AU Miranda, BR Sturtevant, BR Schmelzer, I Doyon, F Wolter, P AF Miranda, Brian R. Sturtevant, Brian R. Schmelzer, Isabelle Doyon, Frederik Wolter, Peter TI Vegetation recovery following fire and harvest disturbance in central Labrador - a landscape perspective SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Canada; disturbance patch attributes; land cover; partial least-squared regression (PLS); site productivity ID LEAST-SQUARES REGRESSION; CANADIAN BOREAL FOREST; SOUTHEASTERN LABRADOR; COMPOSITIONAL DATA; BURN SEVERITY; DATA SETS; WILDFIRE; PATTERNS; PACKAGE; CARIBOU AB Understanding vegetation recovery patterns following wildfire and logging disturbance is essential for long-term planning in sustainable forestry. Plot-scale studies indicate differences in revegetation rates and postdisturbance composition in Labrador, Canada, following fire in comparison with harvest but do not necessarily capture the full range of relevant landscape variability. Using a satellite-based land cover classification that distinguishes forest, woodland, shrub, lichen, and bare ground, we applied partial least-squared regression (PLS) to derive empirical models of vegetation dynamics following fire and harvest. Forest recovery rates were found to be generally slow and sensitive to predisturbance land condition and site quality (potential productivity). We found that, although disturbance type was not specifically retained in the model, estimated rates of vegetation recovery were faster for a typical harvest compared with a typical fire (i.e., 50% recovery at 14 years versus 33 years, respectively). Indeed, the model predicts important regeneration delay following fire that appears sensitive to both site quality and area burned. Understanding factors affecting broad-scale vegetation recovery relationships can help guide future sustainable forestry and wildlife habitat initiatives in the region, in part by parameterizing landscape simulation models used for strategic decision support. C1 [Miranda, Brian R.; Sturtevant, Brian R.] US Forest Serv, Northern Res Stn, Inst Appl Ecosyst Studies, 5985 Highway K, Rhinelander, WI 54501 USA. [Schmelzer, Isabelle] Govt Newfoundland & Labrador, Dept Environm & Conservat, Corner Brook, NF, Canada. [Doyon, Frederik] Univ Quebec Outaouais, Inst Sci Foret Temperee, Dept Sci Nat, 58 Rue Principale, Ripon, PQ, Canada. [Wolter, Peter] Iowa State Univ, Nat Resource Ecol & Management, Sci 132 2, Ames, IA 50011 USA. RP Miranda, BR (reprint author), US Forest Serv, Northern Res Stn, Inst Appl Ecosyst Studies, 5985 Highway K, Rhinelander, WI 54501 USA. EM brmiranda@fs.fed.us FU U.S. National Fire Plan FX This research was supported by funding from the U.S. National Fire Plan. We thank Jean-Francois Senecal (University of Quebec in Montreal) for sharing the intermediate ELC data, as well as Darren Jennings and Scott Payne (Government of Newfoundland and Labrador) for generously providing the Labrador disturbance and GFI data sets. We thank John Stanovick (USDA Forest Service) for advice on statistical methods. Christian Messier, Volker Radeloff, James Vogelmann, and four anonymous reviewers provided helpful comments on earlier versions of the manuscript. NR 61 TC 0 Z9 0 U1 15 U2 15 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD AUG PY 2016 VL 46 IS 8 BP 1009 EP 1018 DI 10.1139/cjfr-2015-0516 PG 10 WC Forestry SC Forestry GA DS6OW UT WOS:000380902500003 ER PT J AU Copenheaver, CA Keyser, TL AF Copenheaver, Carolyn A. Keyser, Tara L. TI Frequency of sprout-origin trees in pre-European settlement forests of the southern Appalachian Mountains SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE forest disturbance; oak regeneration; eastern deciduous forest; forest structure ID EASTERN NORTH-AMERICA; OLD-GROWTH FOREST; BLUE RIDGE MOUNTAINS; HARDWOOD FOREST; OAK FORESTS; SOUTHWESTERN VIRGINIA; CUMBERLAND PLATEAU; SITE RELATIONSHIPS; PRESCRIBED FIRE; WITNESS TREES AB We hypothesized that tree form, recorded in historical public land surveys, would provide a valuable proxy record of regeneration patterns during early-European settlement of North America's eastern deciduous forest. To test this hypothesis, we tallied stem form from witness trees used in land survey records in the southern Appalachian Mountains from 13 counties spanning four physiographic provinces: Piedmont, Blue Ridge, Ridge and Valley, and Cumberland Plateau. A total of 3% of witness trees used in the land surveys were of sprout origin. American basswood (Tilia americana L.) exhibited the highest proportion of sprout-origin trees at 12%. Other overstory species with a high proportion of sprout-origin trees were hickory (Carya sp.), red maple (Acer rubrum L.), and sycamore (Platanus occidentalis L.), all with 6% of stems being from sprout origin. Blue Ridge had significantly more sprout-origin trees compared with the other three physiographic provinces. Forests in the southern Appalachian Mountains during the pre-European settlement period had a suite of disturbances that controlled their growth and regeneration; however, most of these disturbances did not result in large-scale tree mortality, and therefore, sprouts were not an important source of regeneration. C1 [Copenheaver, Carolyn A.] Virginia Tech, Dept Forest Resources & Environm Conservat, 232 Cheatham Hall,Mail Code 0324, Blacksburg, VA 24060 USA. [Keyser, Tara L.] US Forest Serv, USDA, Southern Res Stn, Bent Creek Expt Forest, 1577 Brevard Rd, Asheville, NC 28806 USA. RP Copenheaver, CA (reprint author), Virginia Tech, Dept Forest Resources & Environm Conservat, 232 Cheatham Hall,Mail Code 0324, Blacksburg, VA 24060 USA. EM ccopenhe@vt.edu FU McIntire-Stennis Capacity Grant FX The authors acknowledge K. Hollandsworth who traveled with the authors to several courthouses and assisted with hand transcription of the early land survey records in courthouses that prohibited electronic equipment. Thanks also to K. Frick for assistance with creating the study area map. Partial funding for this project was provided by a McIntire-Stennis Capacity Grant awarded to C. Copenheaver. NR 59 TC 0 Z9 0 U1 4 U2 4 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD AUG PY 2016 VL 46 IS 8 BP 1019 EP 1025 DI 10.1139/cjfr-2016-0078 PG 7 WC Forestry SC Forestry GA DS6OW UT WOS:000380902500004 ER PT J AU Kretchun, AM Loudermilk, EL Scheller, RM Hurteau, MD Belmecheri, S AF Kretchun, Alec M. Loudermilk, E. Louise Scheller, Robert M. Hurteau, Matthew D. Belmecheri, Soumaya TI Climate and bark beetle effects on forest productivity - linking dendroecology with forest landscape modeling SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE ANPP; net ecosystem production; increment cores; forest simulation model; LANDIS-II ID LAKE TAHOE BASIN; MOUNTAIN PINE-BEETLE; NORTHERN SIERRA-NEVADA; MIXED-CONIFER FORESTS; WESTERN UNITED-STATES; TREE MORTALITY; CARBON DYNAMICS; SITE PRODUCTIVITY; CALIFORNIA; DROUGHT AB In forested systems throughout the world, climate influences tree growth and aboveground net primary productivity (ANPP). The effects of extreme climate events (i.e., drought) on ANPP can be compounded by biotic factors (e.g., insect outbreaks). Understanding the contribution of each of these influences on growth requires information at multiple spatial scales and is essential for understanding regional forest response to changing climate. The mixed conifer forests of the Lake Tahoe Basin, California and Nevada, provide an opportunity to analyze biotic and abiotic influences on ANPP. Our objective was to evaluate the influence of moisture stress (climatic water deficit, CWD) and bark beetles on basin-wide ANPP from 1987 to 2006, estimated through tree core increments and a landscape simulation model (LANDIS-II). Tree ring data revealed that ANPP increased throughout this period and had a nonlinear relationship to water demand. Simulation model results showed that despite increased complexity, simulations that include moderate moisture sensitivity and bark beetle outbreaks most closely approximated the field-derived ANPP similar to CWD relationship. Although bark beetle outbreaks and episodic drought-induced mortality events are often correlated, decoupling them within a simulation model offers insight into assessing model performance, as well as examining how each contributes to total declines in productivity. C1 [Kretchun, Alec M.; Scheller, Robert M.] Portland State Univ, Dept Environm Sci & Management, POB 751, Portland, OR 97207 USA. [Loudermilk, E. Louise] US Forest Serv, USDA, Ctr Forest Disturbance Sci, Southern Res Stn, 320 Green St, Athens, GA USA. [Hurteau, Matthew D.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Belmecheri, Soumaya] Univ Arizona, Tucson, AZ USA. RP Kretchun, AM (reprint author), Portland State Univ, Dept Environm Sci & Management, POB 751, Portland, OR 97207 USA. EM aleckretchun@gmail.com RI Scheller, Robert/B-3135-2009; Hurteau, Matthew/D-2301-2009 OI Hurteau, Matthew/0000-0001-8457-8974 FU Bureau of Land Management; Strategic Environmental Research and Development Program [RC-2243] FX This research was supported using funds provided by the Bureau of Land Management through the sale of public lands as authorized by the Southern Nevada Public Lands Management Act (SNPLMA). The USDA Forest Service, particularly Tiffany van Huysen and Carl Skinner of the Pacific Southwest Research Station, were integral to this work through their support and guidance. We thank Peter Weisberg, University of Nevada-Reno, and Megan Creutzberg, Portland State University, as well as colleagues at the Dynamic Ecosystems and Landscapes Lab at Portland State University. The analysis and writing of this paper was funded in part by the Strategic Environmental Research and Development Program (project RC-2243). We also thank Portland State University for administrative support. NR 67 TC 1 Z9 1 U1 44 U2 48 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD AUG PY 2016 VL 46 IS 8 BP 1026 EP 1034 DI 10.1139/cjfr-2016-0103 PG 9 WC Forestry SC Forestry GA DS6OW UT WOS:000380902500005 ER PT J AU Stanosz, GR Smith, DR Juzwik, J AF Stanosz, Glen R. Smith, Denise R. Juzwik, Jennifer TI Seasonal availability of inoculum of the Heterobasidion root disease pathogen in central Wisconsin SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Heterobasidion irregulare; inoculum; Pinus resinosa ID ANNOSUM; ROT; DEPOSITION AB After deposition of airborne basidiospores, the root disease pathogen Heterobasidion irregulare Garbelotto and Otrosina infects fresh conifer stumps and spreads through root grafts or by root contact to adjacent trees. Infection can be prevented, however, by borate application. Because the need for stump protection depends on inoculum availability, spore trapping was conducted (usually biweekly) from September 2009 through December 2011 in three infested plantations of predominantly red pine (Pinus resinosa Aiton) in central Wisconsin. A semiselective medium in Petri plates was exposed for 1 h in daylight at each of four locations in each plantation. After 7-10 days incubation at 20 degrees C, plates were examined and presence and abundance of colonies of the Spiniger asexual stage were recorded. Heterobasidion irregulare was detected on most dates during the two growing seasons, but colonies were most abundant during late summer, fall, and early winter. Relatively fewer colonies developed on medium exposed during periods of coldest winter temperatures, but colonies of the pathogen did develop frequently on medium exposed at <= 5 degrees C and occasionally on medium exposed at <= 0 degrees C. Biologically based guidelines for stump treatment require additional studies of seasonal factors influencing inoculum availability, in situ spore germination, infection, and establishment of the pathogen. C1 [Stanosz, Glen R.; Smith, Denise R.] Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. [Juzwik, Jennifer] US Forest Serv, Northern Res Stn, USDA, 1561 Lindig Ave, St Paul, MN 55108 USA. RP Stanosz, GR (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM gstanosz@wisc.edu FU USDA Forest Service Special Technology Development Program [NA-2009-02] FX The authors gratefully acknowledge private landowners and the Wisconsin Department of Natural Resources for access to infested plantations and the USDA Forest Service Special Technology Development Program for partial financial support (project NA-2009-02). NR 31 TC 0 Z9 0 U1 4 U2 4 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD AUG PY 2016 VL 46 IS 8 BP 1076 EP 1080 DI 10.1139/cjfr-2016-0136 PG 5 WC Forestry SC Forestry GA DS6OW UT WOS:000380902500011 ER PT J AU Purdy, PH Barbosa, EA Praamsma, CJ Schisler, GJ AF Purdy, P. H. Barbosa, E. A. Praamsma, C. J. Schisler, G. J. TI Modification of trout sperm membranes associated with activation and cryopreservation. Implications for fertilizing potential SO CRYOBIOLOGY LA English DT Article DE Cryopreservation; Fertility; Flow cytometry; Merocyanine 540; Phospholipid; Quanti-iT protein assay; Oncorhynchus clarki; Trout sperm ID PLASMA-MEMBRANE; SPERMATOZOA; FLUIDITY; PROTEIN; HYPERPOLARIZATION; QUANTITATION; MOTILITY; ABILITY; STORAGE AB We investigated the effects of two trout sperm activation solutions on sperm physiology and membrane organization prior to and following cryopreservation using flow cytometry and investigated their impact on in vitro fertility. Overall, frozen-thawed samples had greater phospholipid disorder when compared with fresh samples (high plasma membrane fluidity; P < 0.0001) and sperm activated with water also had high plasma membrane fluidity when compared to sperm activated with Lahnsteiner solution (LAS; P < 0.0001). Following cryopreservation water activated samples had membranes with greater membrane protein disorganization compared with LAS but the membrane protein organization of LAS samples was similar to samples prior to freezing (P < 0.0001). Post-thaw water activation resulted in significant increases in intracellular calcium compared to LAS (P < 0.002). In vitro fertility trials with frozen-thawed milt and LAS activation resulted in greater fertility (45%) compared to water activated samples (10%; P < 0.0001). Higher fertility rates correlated with lower intracellular calcium with water (R-2 = -0.9; P = 0.01) and LAS (R-2 = -0.85; P = 0.03) activation. Greater plasma membrane phospholipid (R-2 = -0.89; P = 0.02) and protein (R-2 = -0.84; P = 0.04) disorder correlated with lower water activation fertility rates. These membrane organization characteristics only approached significance with LAS activation in vitro fertility (P = 0.09, P = 0.06, respectively). Potentially the understanding of sperm membrane reorganizations and the physiology associated with activation following cryopreservation may enable users in a repository or hatchery setting to estimate the fertilizing potential of a sample and determine its value. Published by Elsevier Inc. C1 [Purdy, P. H.] ARS, USDA, NLGRP, Natl Anim Germplasm Program, 1111 S Mason, Ft Collins, CO 80521 USA. [Barbosa, E. A.] Univ Brasilia, BR-70910900 Brasilia, DF, Brazil. [Barbosa, E. A.] Embrapa Genet Resources & Biotechnol, Parque Estacao Biol,PqEB S-N, BR-70770901 Brasilia, DF, Brazil. [Praamsma, C. J.] Fish Res Hatchery, Colorado Parks & Wildlife, 5500 WCR 50C, Bellvue, CO 80512 USA. [Schisler, G. J.] Colorado Parks & Wildlife, 317 West Prospect St, Ft Collins, CO 80526 USA. RP Purdy, PH (reprint author), ARS, USDA, NLGRP, Natl Anim Germplasm Program, 1111 S Mason, Ft Collins, CO 80521 USA. EM phil.purdy@ars.usda.gov; eleonora_vet@hotmail.com; chris.praamsma@state.co.us; george.schisler@state.co.us NR 26 TC 0 Z9 0 U1 2 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0011-2240 EI 1090-2392 J9 CRYOBIOLOGY JI Cryobiology PD AUG PY 2016 VL 73 IS 1 BP 73 EP 79 DI 10.1016/j.cryobiol.2016.05.008 PG 7 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA DS2GL UT WOS:000380580500011 PM 27234987 ER PT J AU Dethier, E Magilligan, FJ Renshaw, CE Nislow, KH AF Dethier, Evan Magilligan, Francis J. Renshaw, Carl E. Nislow, Keith H. TI The role of chronic and episodic disturbances on channel-hillslope coupling: the persistence and legacy of extreme floods SO EARTH SURFACE PROCESSES AND LANDFORMS LA English DT Article ID LARGE WOODY DEBRIS; EARTHQUAKE-INDUCED LANDSLIDES; NEW-ENGLAND; SUSPENDED-SEDIMENT; FLUVIAL PROCESSES; COASTAL RIVERS; STREAM POWER; USA; LANDSCAPE; EROSION AB Landscape form represents the cumulative effects of de-stabilizing events relative to recovery processes. Most geomorphic research has focused on the role of episodic rare events on landscape form with less attention paid to the role and persistence of chronic inputs. To better establish the interplay between chronic and episodic extreme events at regional scales, we used aerial photography and post-flood sediment sampling to assess stream and hillslope response and recovery to a 100-300yr. flood caused by Tropical Storm Irene in New England. Within a 14 000km(2) study area, analysis of aerial photographs indicated that the storm initiated (n=534) and reactivated (n=460) a large number of landslides. These landslides dramatically increased overall estimates of regional erosion rates (from 0.0023mm/yr. without Irene to 0.0072mm/yr. with Irene). Similarly, Irene-generated LWD inputs of 0.25-0.5 trees/km exceeded annual background rates in a single event, and these concentrated inputs (10(1)-10(2) of trees/landslide) are likely to result in large jams and snags that are particularly persistent and geomorphically effective. Finally, we found that landslide scars continue to provide elevated sediment inputs years after the event, as evidenced by sustained higher suspended sediment concentrations in streams with Irene-generated landslides. Overall, our results indicate that infrequent, high-magnitude events have a more important geomorphic role in tectonically stable, more moderate-relief systems than has been previously recognized. Understanding the role of these events has particular relevance in regions such as New England, where the frequency and magnitude of extreme storms is expected to increase. Further, these effects may force reconsideration of conservation and restoration targets (for example in channel form and large wood loading and distribution) in fluvial systems. Copyright (c) 2016 John Wiley & Sons, Ltd. C1 [Dethier, Evan; Renshaw, Carl E.] Dartmouth Coll, Dept Earth Sci, 6105 Hinman, Hanover, NH 03755 USA. [Magilligan, Francis J.] Dartmouth Coll, Dept Geog, Hanover, NH 03755 USA. [Nislow, Keith H.] US Forest Serv, Northeast Res Stn, USDA, Amherst, MA 01003 USA. RP Dethier, E (reprint author), Dartmouth Coll, Dept Earth Sci, 6105 Hinman, Hanover, NH 03755 USA. EM Evan.N.Dethier.GR@Dartmouth.edu FU Geological Society of America student grant; Dartmouth Neukom Institute CompX Faculty Grant; National Science Foundation [BCS-1222531] FX Thanks to Sam Merrens, Keith Kantack, Jake Lyon, and Helen Doyle for assistance in the field and lab, and W. Brian Dade, John Gartner, and James Dietrich for additional guidance. We also thank Stuart Lane for his comments on an initial draft of the paper, and we would like to also thank the input from two anonymous reviewers. This work was partially supported by a Geological Society of America student grant, funding from a Dartmouth Neukom Institute CompX Faculty Grant, and National Science Foundation grant (BCS-1222531). NR 63 TC 2 Z9 2 U1 10 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0197-9337 EI 1096-9837 J9 EARTH SURF PROC LAND JI Earth Surf. Process. Landf. PD AUG PY 2016 VL 41 IS 10 BP 1437 EP 1447 DI 10.1002/esp.3958 PG 11 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DS6IV UT WOS:000380886400011 ER PT J AU LeBlanc, J Uchimiya, M Ramakrishnan, G Castaldi, MJ Orlov, A AF LeBlanc, Jeffrey Uchimiya, Minori Ramakrishnan, Girish Castaldi, Marco J. Orlov, Alexander TI Across-Phase Biomass Pyrolysis Stoichiometry, Energy Balance, and Product Formation Kinetics SO ENERGY & FUELS LA English DT Article ID 2-DIMENSIONAL GAS-CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; COAL DEVOLATILIZATION; FLASH CARBONIZATION; UNIFIED CORRELATION; PROXIMATE ANALYSIS; FUNCTIONAL-GROUPS; HEATING VALUES; SLOW PYROLYSIS; BIO-OILS AB Predictive correlations between reactions occurring in the gas, liquid, and solid phases are necessary to economically utilize the thermochemical conversion of agricultural wastes impacting the food, water, and energy nexus. On the basis of an: empirical mass balance (99.7%), this study established the Overall reaction stoichiometry (C33.42H45.95O20.26N0.22S0.14 = 0.50C(20.08)H(57.21)O(22.46)N(0.20)S(0.22) + 1.72H(2)O + 0.10H(2) + 1.07CH(4) + 0.02C(2)H(4) + 0.06C(2)H(6) + 2.21CO(2) + 2.05CO + 0.28C(63.75)H(32.47)O(3.23)N(0.43)S(0.12)) and energy balance for the slow pyrolysis of lignocellulosic pecan shell waste biomass at 10 degrees C min(-1) up to 500 degrees C. In situ thermogravimetry-gas chromatography and diffuse reflectance infrared fourier transform spectroscopy (DRIFTS) were used to link the gas-, liquid-, and solid-phase nonisothermal reaction kinetics. Gaussian 'fit-based deconvolution of individual gaseous product formation rates (hydrogen, methane, carbon monoxide, carbon dioxide, ethylene, and ethane in mg min(-1)) suggested the relationships between (1) evolved methane and increased aromaticity/energy density of char product at 300-500 degrees C, and (2) evolved carbon dioxide and decarboxylation of char product near 400 degrees C. Partial least-squares (PLS) calibrations were obtained between (1) DRIFTS monitoring of the surface functional groups in the solid phase (transition from pecan shell to char) and (2) CO, CO2, CH4, C2H6, C2H4, and tar formation profiles in the gas/condensable phase. Established across-phase PLS calibrations can be used to predict biochar's surface chemistry based on the fingerprint of volatile products, and vice versa. These new thermodynamic (reaction stoichiometry and energy balance) and kinetic (deconvolution of specific gas formation rates and PLS) predictive methodologies will facilitate the nexus of food, water (designing of biochar soil amendment), and energy (optimization of syngas and bio-oil composition) enabling sustainable agriculture. C1 [LeBlanc, Jeffrey; Castaldi, Marco J.] CUNY City Coll, Dept Chem Engn, 140th St,Convent Ave Steinman Hall,Room 307, New York, NY 10031 USA. [Uchimiya, Minori; Ramakrishnan, Girish; Orlov, Alexander] SUNY Stony Brook, Dept Mat Sci & Engn, Room 314, Stony Brook, NY 11794 USA. [Uchimiya, Minori] USDA ARS, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP Castaldi, MJ (reprint author), CUNY City Coll, Dept Chem Engn, 140th St,Convent Ave Steinman Hall,Room 307, New York, NY 10031 USA.; Uchimiya, M (reprint author), SUNY Stony Brook, Dept Mat Sci & Engn, Room 314, Stony Brook, NY 11794 USA.; Uchimiya, M (reprint author), USDA ARS, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov; mcastaldi@ccny.cuny.edu NR 49 TC 1 Z9 1 U1 13 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD AUG PY 2016 VL 30 IS 8 BP 6537 EP 6546 DI 10.1021/acs.energyfuels.6b01376 PG 10 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA DT8XH UT WOS:000381778500038 ER PT J AU Rosenberger, DW Venette, RC Aukema, BH AF Rosenberger, Derek W. Venette, Robert C. Aukema, Brian H. TI Sexing live mountain pine beetles Dendroctonus ponderosae: refinement of a behavioral method for Dendroctonus spp. SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Editorial Material DE bark beetle; plectrum; pars stridens; stress chirp; Hylurgini; Scolytinae ID SOUND PRODUCTION; BARK BEETLES; ACOUSTIC-SIGNALS; SCOLYTIDAE; COLEOPTERA; STRIDULATION; CURCULIONIDAE; ECOLOGY C1 [Rosenberger, Derek W.; Aukema, Brian H.] Univ Minnesota, Dept Entomol, 1980 Folwell Ave, St Paul, MN 55108 USA. [Venette, Robert C.] US Forest Serv, USDA, Northern Res Stn, St Paul, MN USA. RP Rosenberger, DW (reprint author), Univ Minnesota, Dept Entomol, 1980 Folwell Ave, St Paul, MN 55108 USA. EM rose0675@umn.edu NR 30 TC 1 Z9 1 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD AUG PY 2016 VL 160 IS 2 BP 195 EP 199 DI 10.1111/eea.12463 PG 5 WC Entomology SC Entomology GA DT7UI UT WOS:000381692400011 ER PT J AU Nigatu, G Dinar, A AF Nigatu, Getachew Dinar, Ariel TI Economic and hydrological impacts of the Grand Ethiopian Renaissance Dam on the Eastern Nile River Basin SO ENVIRONMENT AND DEVELOPMENT ECONOMICS LA English DT Article ID BLUE NILE; PROPERTY-RIGHTS; CLIMATE-CHANGE; WATER; AFRICA; RESOURCES; OPTIONS AB We propose an allocate-and-trade' institution to manage the eastern Nile River Basin for Ethiopia, Sudan and Egypt as the basin faces a new reality of the Grand Ethiopian Renaissance Dam (GERD). We find that a social planner could increase the region's economic welfare by assigning water rights to the riparian states. An alternative intrabasin water rights arrangement and trade could achieve more than 95 per cent of the welfare created by the social planner. GERD will change both the economic benefits and hydrological positions of the riparian countries. Economic benefits from alternative water use would be sufficient to make riparian countries better off compared with the status quo. Furthermore, riparian countries could raise more than US$680 m annually for protecting and conserving the natural resources of the region. C1 [Nigatu, Getachew] ERS, Market & Trade Econ Div, USDA, 335 E St SW, Washington, DC 20024 USA. [Dinar, Ariel] Univ Calif Riverside, Sch Publ Policy, Riverside, CA 92521 USA. RP Nigatu, G (reprint author), ERS, Market & Trade Econ Div, USDA, 335 E St SW, Washington, DC 20024 USA. EM gsnigatu@ers.sda.gov; ariel.dinar@ucr.edu NR 64 TC 0 Z9 0 U1 17 U2 17 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 1355-770X EI 1469-4395 J9 ENVIRON DEV ECON JI Environ. Dev. Econ. PD AUG PY 2016 VL 21 IS 4 BP 532 EP 555 DI 10.1017/S1355770X15000352 PG 24 WC Environmental Studies SC Environmental Sciences & Ecology GA DS6SQ UT WOS:000380913200006 ER PT J AU Cook, DE Combs, DK Doane, PH Cecava, MJ Hall, MB AF Cook, D. E. Combs, D. K. Doane, P. H. Cecava, M. J. Hall, M. B. TI The effects on digestibility and ruminal measures of chemically treated corn stover as a partial replacement for grain in dairy diets SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE corn stover; calcium oxide; alkaline; fiber digestibility ID ALKALINE HYDROGEN-PEROXIDE; NEUTRAL DETERGENT FIBER; WHEAT-STRAW; DRY-MATTER; IN-VITRO; COWS; DIGESTION; MODELS; SILAGE; PERFORMANCE AB Alkaline treatment of gramineous crop residues can convert an abundant, minimally utilized, poorly digestible straw into a moderately digestible feedstuff. Given the volatile nature of grain prices, substitution of treated stover for grain was investigated with dairy cows to provide insights on ruminal and digestibility effects of a feed option that makes use of alternative, available resources. The objective of this study was to evaluate changes in diet digestibility and ruminal effects when increasing levels of calcium oxide-treated corn stover (CaOSt) were substituted for corn grain in diets of lactating cows. Mature corn stover was treated with calcium oxide at a level of 50 g.kg(-1) dry matter (DM), brought up to a moisture content of 50% following bale grinding, and stored anaerobically at ambient temperatures for greater than 60 d before the feeding experiment. Eight ruminally cannulated Holstein cows averaging 686 kg of body weight and 35 kg of milk.d(-1) were enrolled in a replicated 4 x 4 Latin square, where CaOSt replaced corn grain on a DM basis in the ration at rates of 0, 40, 80, and 120 g.kg(-1) DM. All reported significant responses were linear. The DM intake declined by approximately 1 kg per 4% increase in CaOSt inclusion. With increasing replacement of corn grain, dietary neutral detergent fiber (NDF) concentration increased. However, rumen NDF turnover, NDF digestibility, NDF passage rate, and digestion rate of potentially digestible NDF were unaffected by increasing CaOSt inclusion. Total-tract organic matter digestibility declined by 5 percentage units over the range of treatments, approximately 1.5 units per 4-percentageunit substitution of CaOSt for grain. With increasing CaOSt, the molar proportions of butyrate and valerate declined, whereas the lowest detected ruminal pH increased from 5.83 to 5.94. Milk, fat, and protein yields declined as CaOSt increased and DM intake declined with the result that net energy in milk declined by approximately 1 Mcal per 4% increase in CaOSt. Time spent ruminating (min.kg(-1) DM intake) increased with increasing CaOSt, though total minutes per day were unaffected. These insights on the effect of substitution of treated corn stover for corn grain may be used to predict the effect on nutrient supply to the cow over a range of substitution levels. The acceptability of the effect will depend on the economics of milk production and availabilities of feedstuffs. C1 [Cook, D. E.; Combs, D. K.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Doane, P. H.; Cecava, M. J.] ADM Res, Decatur, IL 62526 USA. [Hall, M. B.] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. RP Hall, MB (reprint author), ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. EM marybeth.hall@ars.usda.gov FU ADM Company (Decatur, IL) FX We thank the ADM Company (Decatur, IL) for the funding that supported this project, and J. W. Pitas of the USDA Agricultural Research Service (Madison, WI) for technical support. NR 31 TC 0 Z9 0 U1 7 U2 7 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2016 VL 99 IS 8 BP 6342 EP 6351 DI 10.3168/jds.2015-10403 PG 10 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DT7BY UT WOS:000381642500035 PM 27320677 ER PT J AU Gaddis, KLP Null, DJ Cole, JB AF Gaddis, K. L. Parker Null, D. J. Cole, J. B. TI Explorations in genome-wide association studies and network analyses with dairy cattle fertility traits SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE fertility; genome-wide association; high-density genotypes; network analysis ID GENE COEXPRESSION NETWORKS; HOLSTEIN-FRIESIAN CATTLE; MATERNAL REPRODUCTION; EMBRYONIC-DEVELOPMENT; IN-VITRO; BOVINE; COWS; INFORMATION; EXPRESSION; HEALTH AB The objective of this study was to identify single nucleotide polymorphisms and gene networks associated with 3 fertility traits in dairy cattle-daughter pregnancy rate, heifer conception rate, and cow conception rate-using different approaches. Deregressed predicted transmitting abilities were available for approximately 24,000 Holstein bulls and 36,000 Holstein cows sampled from the National Dairy Database with high-density genotypes. Of those, 1,732 bulls and 375 cows had been genotyped with the Illumina BovineHD Genotyping BeadChip (Illumina Inc., San Diego, CA). The remaining animals were genotyped with various chips of lower density that were imputed to high density. Univariate and trivariate genome-wide association studies (GWAS) with both medium- (60,671 markers) and high-density (312,614 markers) panels were performed for daughter pregnancy rate, heifer conception rate, and cow conception rate using GEMMA (version 0.94; http://www.xzlab.org/software.html). Analyses were conducted using bulls only, cows only, and a sample of both bulls and cows. The partial correlation and information theory algorithm was used to develop gene interaction networks. The most significant markers were further investigated to identify putatively associated genes. Little overlap in associated genes could be found between GWAS using different reference populations of bulls only, cows only, and combined bulls and cows. The partial correlation and information theory algorithm was able to identify several genes that were not identified by ordinary GWAS. The results obtained herein will aid in further dissecting the complex biology underlying fertility traits in dairy cattle, while also providing insight into the nuances of GWAS. C1 [Gaddis, K. L. Parker] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Null, D. J.; Cole, J. B.] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Gaddis, KLP (reprint author), Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. EM klpgaddis@ufl.edu RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Agriculture and Food Research Initiative Grant from the National Institute of Food and Agriculture (Washington, DC) [2013-68004-20365]; Agricultural Research Service of the United States Department of Agriculture (Washington, DC) [1265-31000-096-00] FX The Council on Dairy Cattle Breeding (Reynoldsburg, OH) and the Cooperative Dairy DNA Repository (Columbia, MO) are acknowledged for providing data used in this study. Financial support was provided by Agriculture and Food Research Initiative Grant 2013-68004-20365, "Improving Fertility of Dairy Cattle Using Translational Genomics," from the National Institute of Food and Agriculture (Washington, DC). J. B. Cole and D. J. Null also were supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information," of the Agricultural Research Service of the United States Department of Agriculture (Washington, DC). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 77 TC 0 Z9 0 U1 5 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2016 VL 99 IS 8 BP 6420 EP 6435 DI 10.3168/jds.2015-10444 PG 16 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DT7BY UT WOS:000381642500042 ER PT J AU Adams, HA Sonstegard, TS VanRaden, PM Null, DJ Van Tassell, CP Larkin, DM Lewin, HA AF Adams, Heather A. Sonstegard, Tad S. VanRaden, Paul M. Null, Daniel J. Van Tassell, Curt P. Larkin, Denis M. Lewin, Harris A. TI Identification of a nonsense mutation in APAF1 that is likely causal for a decrease in reproductive efficiency in Holstein dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE nonsense mutation; APAF1; dairy cattle; resequencing ID COMPLEX VERTEBRAL MALFORMATION; CELL-DEATH; DEFICIENCY; SELECTION; HAPLOTYPES; APOPTOSOME; FERTILITY; SYNTHASE; MARKERS; CALVES AB The HH1 haplotype on chromosome 5 is associated with a reduced conception rate and a deficit of homozygotes at the population level in Holstein cattle. The source HH1 haplotype was traced to the bull Pawnee Farm Arlinda Chief (Chief), who was born in 1962 and has sired more than 16,000 daughters. We identified a nonsense mutation in APAF1 (apoptotic protease activating factor 1; APAF1 p.Q579X) within HH1 using whole-genome resequencing of Chief and 3 of his sons. This mutation is predicted to truncate 670 AA (53.7%) of the encoded APAF1 protein that contains a WD40 domain critical to protein-protein interactions. Initial screening revealed no homozygous individuals for the mutation in 758 animals previously genotyped, whereas all 497 HH1 carriers possessed 1 copy of the mutant allele. Subsequent commercial genotyping of 246,773 Holsteins revealed 5,299 APAF1 heterozygotes and zero homozygotes for the mutation. The causative role of this mutation is also supported by functional data in mice that have demonstrated Apaf1 to be an essential molecule in the cytochrome-c-mediated apoptotic cascade and directly implicated in developmental and neurodegenerative disorders. In addition, most Apaf1 homozygous knockouts die by day 16.5 of development. We thus propose that the APAF1 p.Q579X nonsense mutation is the functional equivalent of the Apaf1 knockout. This mutation has caused an estimated 525,000 spontaneous abortions worldwide over the past 35 years, accounting for approximately $420 million in losses. With the mutation identified, selection against the deleterious allele in breeding schemes has aided in eliminating this defect from the population, reducing carrier frequency from 8% in past decades to 2% in 2015. C1 [Adams, Heather A.; Larkin, Denis M.; Lewin, Harris A.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Adams, Heather A.; Lewin, Harris A.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Sonstegard, Tad S.; VanRaden, Paul M.; Null, Daniel J.; Van Tassell, Curt P.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. [Lewin, Harris A.] Univ Calif Davis, Dept Ecol & Evolut, Davis, CA 95616 USA. [Lewin, Harris A.] Univ Calif Davis, UC Davis Genome Ctr, Davis, CA 95616 USA. [Adams, Heather A.; Lewin, Harris A.] CRI Int Ctr Biotechnol, Mt Horeb, WI 53572 USA. [Sonstegard, Tad S.] Acceligen Recombinet Inc, St Paul, MN 55104 USA. RP Lewin, HA (reprint author), Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA.; Lewin, HA (reprint author), Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA.; Lewin, HA (reprint author), Univ Calif Davis, Dept Ecol & Evolut, Davis, CA 95616 USA.; Lewin, HA (reprint author), Univ Calif Davis, UC Davis Genome Ctr, Davis, CA 95616 USA.; Lewin, HA (reprint author), CRI Int Ctr Biotechnol, Mt Horeb, WI 53572 USA. EM lewin@ucdavis.edu FU USDA Agricultural Research Service CRIS [1265-31000-104-00D, 31S] FX This work was supported by USDA Agricultural Research Service CRIS projects 1265-31000-104-00D and 31S. The authors thank Gene McCoy (University of Illinois at Urbana-Champaign) and A. Beavers and M. McClure (Animal Genomics and Improvement Laboratory, Agricultural Research Service, USDA, Beltsville, MD) for assistance, as well as GeneSeek-Neogen Corp. (Lincoln, NE) for providing supporting genotype data. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (Washington, DC). NR 32 TC 1 Z9 1 U1 5 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD AUG PY 2016 VL 99 IS 8 BP 6693 EP 6701 DI 10.3168/jds.2015-10517 PG 9 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA DT7BY UT WOS:000381642500067 PM 27289157 ER PT J AU Myer, PR Kim, M Freetly, HC Smith, TPL AF Myer, Phillip R. Kim, MinSeok Freetly, Harvey C. Smith, Timothy P. L. TI Evaluation of 16S rRNA amplicon sequencing using two next-generation sequencing technologies for phylogenetic analysis of the rumen bacterial community in steers SO JOURNAL OF MICROBIOLOGICAL METHODS LA English DT Article DE 16S rRNA gene; MiSeq; OTUs; PacBio; Rumen Microbiome ID MICROBIAL COMMUNITIES; FEED-EFFICIENCY; DAIRY-COWS; GENE; PCR; DIVERSITY; PRIMERS; DYNAMICS; REGIONS; SAMPLES AB Next generation sequencing technologies have vastly changed the approach of sequencing of the 16S rRNA gene for studies in microbial ecology. Three distinct technologies are available for large-scale 16S sequencing. All three are subject to biases introduced by sequencing error rates, amplification primer selection, and read length, which can affect the apparent microbial community. In this study, we compared short read 16S rRNA variable regions, V1-V3, with that of near-full length 16S regions, V1-V8, using highly diverse steer rumen microbial communities, in order to examine the impact of technology selection on phylogenetic profiles. Short paired-end reads from the Illumina MiSeq platform were used to generate V1-V3 sequence, while long "circular consensus" reads from the Pacific Biosciences RSII instrument were used to generate V1-V8 data. The two platforms revealed similar microbial operational taxonomic units (OTUs), as well as similar species richness, Good's coverage, and Shannon diversity metrics. However, the V1-V8 amplified ruminal community resulted in significant increases in several orders of taxa, such as phyla Proteobacteria and Verrucomicrobia (P < 0.05). Taxonomic classification accuracy was also greater in the near full-length read. UniFrac distance matrices using jackknifed UPGMA clustering also noted differences between the communities. These data support the consensus that longer reads result in a finer phylogenetic resolution that may not be achieved by shorter 16S rRNA gene fragments. Our work on the cattle rumen bacterial community demonstrates that utilizing near full-length 16S reads may be useful in conducting a more thorough study, or for developing a niche-specific database to use in analyzing data from shorter read technologies when budgetary constraints preclude use of near-full length 16S sequencing. (C) 2016 Elsevier B.V. All rights reserved. C1 [Myer, Phillip R.] Univ Tennessee, Inst Agr, Dept Anim Sci, Knoxville, TN 37996 USA. [Kim, MinSeok; Freetly, Harvey C.; Smith, Timothy P. L.] USDA ARS, Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Myer, PR (reprint author), Univ Tennessee, Inst Agr, Dept Anim Sci, Knoxville, TN 37996 USA. EM pmyer@utk.edu; mkim2276@korea.kr; harvey.freetly@ars.usda.gov; tim.smith@ars.usda.gov FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68004-30214] FX We thank Bob Lee, Sue Hauver, Kelsey McClure, Renee Godtel, and Brooke Clemmons for technical assistance. This project is partially supported by Agriculture and Food Research Initiative Competitive Grant No. 2011-68004-30214 from the USDA National Institute of Food and Agriculture. NR 48 TC 2 Z9 2 U1 32 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-7012 EI 1872-8359 J9 J MICROBIOL METH JI J. Microbiol. Methods PD AUG PY 2016 VL 127 BP 132 EP 140 DI 10.1016/j.mimet.2016.06.004 PG 9 WC Biochemical Research Methods; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA DS2PA UT WOS:000380624800022 PM 27282101 ER PT J AU Lustgarten, MS Price, LL Phillips, EM Kirn, DR Mills, J Fielding, RA AF Lustgarten, Michael S. Price, Lori L. Phillips, Edward M. Kirn, Dylan R. Mills, John Fielding, Roger A. TI SERUM PREDICTORS OF PERCENT LEAN MASS IN YOUNG ADULTS SO JOURNAL OF STRENGTH AND CONDITIONING RESEARCH LA English DT Article DE analytes; metabolites; metabolomics; lean mass ID OXIDATIVE STRESS; INSULIN-RESISTANCE; BODY-COMPOSITION; SKELETAL-MUSCLE; IDENTIFICATION; METABOLITES; PROTEIN; HUMANS; HEALTH; ACID AB Elevated lean (skeletal muscle) mass is associated with increased muscle strength and anaerobic exercise performance, whereas low levels of lean mass are associated with insulin resistance and sarcopenia. Therefore, studies aimed at obtaining an improved understanding of mechanisms related to the quantity of lean mass are of interest. Percent lean mass (total lean mass/body weight x 100) in 77 young subjects (18-35 years) was measured with dual-energy x-ray absorptiometry. Twenty analytes and 296 metabolites were evaluated with the use of the standard chemistry screen and mass spectrometry-based metabolomic profiling, respectively. Sex-adjusted multivariable linear regression was used to determine serum analytes and metabolites significantly (p <= 0.05 and q <= 0.30) associated with the percent lean mass. Two enzymes (alkaline phosphatase and serum glutamate oxaloacetate aminotransferase) and 29 metabolites were found to be significantly associated with the percent lean mass, including metabolites related to microbial metabolism, uremia, inflammation, oxidative stress, branched-chain amino acid metabolism, insulin sensitivity, glycerolipid metabolism, and xenobiotics. Use of sex-adjusted stepwise regression to obtain a final covariate predictor model identified the combination of 5 analytes and metabolites as overall predictors of the percent lean mass (model R-2 = 82.5%). Collectively, these data suggest that a complex interplay of various metabolic processes underlies the maintenance of lean mass in young healthy adults. C1 [Lustgarten, Michael S.; Phillips, Edward M.; Kirn, Dylan R.; Mills, John; Fielding, Roger A.] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Price, Lori L.] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Biostat Res Ctr, Boston, MA USA. RP Fielding, RA (reprint author), Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM roger.fielding@tufts.edu FU DOD [W911SR06C0001]; U.S. Department of Agriculture [58-1950-0-014] FX This work was supported by DOD contract W911SR06C0001 and in part by the U.S. Department of Agriculture, under agreement 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 45 TC 0 Z9 0 U1 1 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 1064-8011 EI 1533-4287 J9 J STRENGTH COND RES JI J. Strength Cond. Res. PD AUG PY 2016 VL 30 IS 8 BP 2194 EP 2201 PG 8 WC Sport Sciences SC Sport Sciences GA DS4LO UT WOS:000380752800015 PM 23774283 ER PT J AU Herring, D Tagtow, A AF Herring, David Tagtow, Angie TI MyPlate, MyState-Personalizing Your Plate with Your Local Flavors, Foods, and Recipes SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Herring, David; Tagtow, Angie] USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. RP Herring, D (reprint author), USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD AUG PY 2016 VL 116 IS 8 BP 1239 EP 1240 DI 10.1016/j.jand.2016.06.004 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DS5MY UT WOS:000380827600003 PM 27469522 ER PT J AU Blitstein, JL Cates, SC Hersey, J Montgomery, D Shelley, M Hradek, C Kosa, K Bell, L Long, V Williams, PA Olson, S Singh, A AF Blitstein, Jonathan L. Cates, Sheryl C. Hersey, James Montgomery, Doris Shelley, Mack Hradek, Christine Kosa, Katherine Bell, Loren Long, Valerie Williams, Pamela A. Olson, Sara Singh, Anita TI Adding a Social Marketing Campaign to a School-Based Nutrition Education Program Improves Children's Dietary Intake: A Quasi-Experimental Study SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Supplemental Nutrition Assistance Program (SNAP); Child; Nutrition education; Social marketing; Intervention ID TYPE-2 DIABETES-MELLITUS; VEGETABLE CONSUMPTION; FOOD ENVIRONMENT; CORONARY ATHEROSCLEROSIS; BEVERAGE CONSUMPTION; PARENTAL INFLUENCE; CHILDHOOD OBESITY; UNITED-STATES; RISK-FACTORS; FRUIT AB Background Evidence supports the use of social marketing campaigns to improve nutrition knowledge and reinforce the effects of nutrition education programs. However, the additional effects of parent-focused social marketing with nutrition education have received little attention. Objective Our aim was to assess the impact of the Iowa Nutrition Network's school-based nutrition education program (Building and Strengthening Iowa Community Support for Nutrition and Physical Activity [BASICS]) and the benefits of adding a multichannel social marketing intervention (BASICS Plus) to increase parent-directed communication. Design and intervention A quasi-experimental design with three study conditions compared a school-based nutrition education program (BASICS) with a school-based and social marketing intervention (BASICS Plus) and a no-treatment comparison group. Participants/setting The study included 1,037 third-grade students attending 33 elementary schools and their parents. Main outcome measures Measures included parents' reports of their children's in-home consumption of fruits and vegetables (F/V) and use of low-fat/fat-free milk. Data on F/V were collected using a modified version of the University of California Cooperative Extension Food Behavior Checklist; and data on milk use were collected using two questions from the National Health and Nutrition Examination Survey. Statistical analyses Multilevel, mixed-effect regression models that account for correlation within repeated measures and children within school were used to compare the mean change over time in the outcome variable for one study group with the mean change over time for another study group. Results Children in BASICS increased mean consumption of fruit by 0.16 cups (P = 0.04) compared with children in the comparison group. Children in BASICS Plus increased mean consumption of fruit by 0.17 cups (P = 0.03) and mean consumption of vegetables by 0.13 cups (P = 0.02). Children in BASICS Plus were 1.3 times (P = 0.05) more likely to use low-fat/fat-free milk than children in either the BASICS group or the comparison group. Conclusions Gaining parents' attention and engaging them in healthy eating practices for their children can be a useful way to increase the effectiveness of school-based nutrition education programs. This study demonstrates the benefits of incorporating a parent-focused social marketing campaign in nutrition education interventions. C1 [Blitstein, Jonathan L.; Cates, Sheryl C.; Hersey, James; Kosa, Katherine; Williams, Pamela A.] RTI Int, 3040 East Cornwallis Rd, Res Triangle Pk, NC 27709 USA. [Shelley, Mack] Iowa State Univ, Polit Sci, Stat, Ames, IA USA. [Shelley, Mack] Iowa State Univ, Sch Educ, Ames, IA USA. [Shelley, Mack] Iowa State Univ, Dept Polit Sci, Ames, IA USA. [Hradek, Christine] Iowa State Univ Cooperat Extens, Ames, IA USA. [Montgomery, Doris; Hradek, Christine] Iowa Dept Publ Hlth, Des Moines, IA 50319 USA. [Bell, Loren; Long, Valerie] Altarum Inst, Ctr Food Assistance & Nutr, Portland, ME USA. [Olson, Sara] Food & Nutr Serv, Child Nutr Programs, USDA, Alexandria, VA USA. [Singh, Anita] Food & Nutr Serv, SNAP Evaluat Branch, USDA, Alexandria, VA USA. RP Blitstein, JL (reprint author), RTI Int, 3040 East Cornwallis Rd, Res Triangle Pk, NC 27709 USA. EM jblitstein@rti.org FU US Department of Agriculture's Food and Nutrition Service [AG-3198-D-09-0096] FX This research was supported by US Department of Agriculture's Food and Nutrition Service contract AG-3198-D-09-0096. The opinions expressed in this article are those of the authors and do not necessarily represent the views of the Food and Nutrition Service or the US Department of Agriculture. NR 63 TC 0 Z9 0 U1 24 U2 29 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 EI 2212-2680 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD AUG PY 2016 VL 116 IS 8 BP 1285 EP 1294 DI 10.1016/j.jand.2015.12.016 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA DS5MY UT WOS:000380827600009 PM 26857870 ER PT J AU Dobrowski, SZ Parks, SA AF Dobrowski, Solomon Z. Parks, Sean A. TI Climate change velocity underestimates climate change exposure in mountainous regions SO NATURE COMMUNICATIONS LA English DT Article ID PLANT-COMMUNITIES; NORTH-AMERICA; CONSERVATION; MICROREFUGIA; CHALLENGES; SURFACES; REFUGIA; MARINE; PACE AB Climate change velocity is a vector depiction of the rate of climate displacement used for assessing climate change impacts. Interpreting velocity requires an assumption that climate trajectory length is proportional to climate change exposure; longer paths suggest greater exposure. However, distance is an imperfect measure of exposure because it does not quantify the extent to which trajectories traverse areas of dissimilar climate. Here we calculate velocity and minimum cumulative exposure (MCE) in degrees Celsius along climate trajectories for North America. We find that velocity is weakly related to MCE; each metric identifies contrasting areas of vulnerability to climate change. Notably, velocity underestimates exposure in mountainous regions where climate trajectories traverse dissimilar climates, resulting in high MCE. In contrast, in flat regions velocity is high where MCE is low, as these areas have negligible climatic resistance to movement. Our results suggest that mountainous regions are more climatically isolated than previously reported. C1 [Dobrowski, Solomon Z.] Univ Montana, Coll Forestry & Conservat, Dept Forest Management, Missoula, MT 59812 USA. [Parks, Sean A.] US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, Missoula, MT 59812 USA. RP Dobrowski, SZ (reprint author), Univ Montana, Coll Forestry & Conservat, Dept Forest Management, Missoula, MT 59812 USA.; Parks, SA (reprint author), US Forest Serv, Aldo Leopold Wilderness Res Inst, Rocky Mt Res Stn, Missoula, MT 59812 USA. EM solomon.dobrowski@umontana.edu; sean_parks@fs.fed.us FU National Science Foundation [DEB 1145985, BCS 1461576]; USFS Rocky Mountain Research Station [15-JV-11221639-119] FX S.Z.D. was supported by the National Science Foundation (DEB 1145985; BCS 1461576) and USFS Rocky Mountain Research Station (Agreement number: 15-JV-11221639-119). We thank Brady Allred for providing computing resources, and John Abatzoglou and three anonymous reviewers for constructive comments on previous drafts of this manuscript. NR 40 TC 0 Z9 0 U1 19 U2 19 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD AUG PY 2016 VL 7 AR 12349 DI 10.1038/ncomms12349 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS5WN UT WOS:000380853800001 PM 27476545 ER PT J AU Cowger, C Weisz, R Arellano, C Murphy, P AF Cowger, Christina Weisz, Randy Arellano, Consuelo Murphy, Paul TI Profitability of Integrated Management of Fusarium Head Blight in North Carolina Winter Wheat SO PHYTOPATHOLOGY LA English DT Article ID GIBBERELLA-ZEAE; TEST WEIGHT; DEOXYNIVALENOL; METAANALYSIS; DISEASE; YIELD; GRAIN; INTENSITY; FUNGICIDE; BARLEY AB Fusarium head blight (FHB) is one of the most difficult small-grain diseases to manage, due to the partial effectiveness of management techniques and the narrow window of time in which to apply fungicides profitably. The most effective management approach is to integrate cultivar resistance with FHB-specific fungicide applications; yet, when forecasted risk is intermediate, it is often unclear whether such an application will be profitable. To model the profitability of FHB management under varying conditions, we conducted a 2-year split-plot field experiment having as main plots high-yielding soft red winter wheat cultivars, four moderately resistant (MR) and three susceptible (S) to FHB. Subplots were sprayed at flowering with Prosaro or Caramba, or left untreated. The experiment was planted in seven North Carolina environments (location-year combinations); three were irrigated to promote FHB development and four were not irrigated. Response variables were yield, test weight, disease incidence, disease severity, deoxynivalenol (DON), Fusarium-damaged kernels, and percent infected kernels. Partial profits were compared in two ways: first, across low-, medium-, or high-DON environments; and second, across environment-cultivar combinations divided by risk forecast into "do spray" and "do not spray" categories. After surveying DON and test weight dockage among 21 North Carolina wheat purchasers, three typical market scenarios were used for modeling profitability: feed-wheat, flexible (feed or flour), and the flour market. A major finding was that, on average, MR cultivars were at least as profitable as S cultivars, regardless of epidemic severity or market. Fungicides were profitable in the feed-grain and flexible markets when DON was high, with MR cultivars in the flexible or flour markets when DON was intermediate, and on S cultivars aimed at the flexible market. The flour market was only profitable when FHB was present if DON levels were intermediate and cultivar resistance was combined with a fungicide. It proved impossible to use the risk forecast to predict profitability of fungicide application. Overall, the results indicated that cultivar resistance to FHB was important for profitability, an FHB-targeted fungicide expanded market options when risk was moderate or high, and the efficacy of fungicide decision-making is reduced by factors that limit the accuracy of risk forecasts. C1 [Cowger, Christina] North Carolina State Univ, USDA ARS, Dept Plant Pathol, Raleigh, NC 27695 USA. [Weisz, Randy; Murphy, Paul] North Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Arellano, Consuelo] North Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. RP Cowger, C (reprint author), North Carolina State Univ, USDA ARS, Dept Plant Pathol, Raleigh, NC 27695 USA. EM Christina.Cowger@ars.usda.gov NR 22 TC 0 Z9 0 U1 13 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2016 VL 106 IS 8 BP 814 EP 823 DI 10.1094/PHYTO-10-15-0263-R PG 10 WC Plant Sciences SC Plant Sciences GA DS5DS UT WOS:000380801600003 PM 27111803 ER PT J AU Rashed, A Wallis, CM Workneh, F Paetzold, L Rush, CM AF Rashed, Arash Wallis, Christopher M. Workneh, Fekede Paetzold, Li Rush, Charles M. TI Variations in Zebra Chip Disease Expression and Tuber Biochemistry in Response to Vector Density SO PHYTOPATHOLOGY LA English DT Article ID CANDIDATUS LIBERIBACTER SOLANACEARUM; TRANSMISSION EFFICIENCY; POTATO DISEASE; UNITED-STATES; INFECTION; CITRUS; TIME; INOCULATION; ASSOCIATION; MANAGEMENT AB Zebra chip (ZC) disease, caused by 'Candidatus Liberibacter solanacearum', which is transmitted by the potato psyllid, has negatively affected potato production in the United States for over a decade. The present study was conducted to evaluate the affect of the number of bacteriliferous psyllids on 'Ca. L. solanacearum' titer, levels of amino acids, carbohydrates, phenolics, and, subsequently, symptom severity in potato tubers. 'Red La Soda' and 'Russet Norkotah' potato were planted in the field and later inoculated with 'Ca. L. solanacearum' using 5, 10, and 30 bacteriliferous potato psyllids. In both cultivars, the increase in the number of psyllids resulted in elevated 'Ca. L. solanacearum' titer and symptom severity. In the cases of amino acids and reducing sugars, responses to vector density appeared to be cultivar specific. Overall, phenolic compounds showed a consistent increase following infection, a response that, in most cases, was positively correlated with the number of infective psyllids. Results quantified the impact of the number of infective psyllids on 'Ca. L. solanacearum' titer and biochemical content of infected tubers previously shown to be correlated with the degree of symptom expression. Managing vector numbers is currently the only effective approach for minimizing losses to ZC in grower's fields. However, our findings on physiological responses to vector density suggest that, in combination with chemical control, development of more tolerant or resistant potato genotypes, with emphasis on interrupting pathways that are associated with increased phenolic activity levels, may lead to more sustainable management of ZC in the future. C1 [Rashed, Arash] Univ Idaho, Dept Plant Soil & Entomol Sci, Aberdeen Res & Extens Ctr, Aberdeen, ID 83210 USA. [Wallis, Christopher M.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Crop Dis Pests & Genet Res Unit, 9611 S Riverbend Ave, Parlier, CA 93648 USA. [Workneh, Fekede; Paetzold, Li; Rush, Charles M.] Texas A&M Agrilife Res & Extens, 6500 Amarillo Blvd W, Amarillo, TX 79106 USA. RP Rashed, A (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, Aberdeen Res & Extens Ctr, Aberdeen, ID 83210 USA. EM arashed@uidaho.edu FU Texas Department of Agriculture Zebra Chip Initiative [ZC-1213-01]; USDA Specialty Crop Research Initiative [2009-511-20176]; USDA National Institute of Food and Agriculture Hatch [IDA01506] FX We thank our lab members for their help with different aspects of this project and the Texas Department of Agriculture Zebra Chip Initiative (number ZC-1213-01), USDA Specialty Crop Research Initiative (number 2009-511-20176), and USDA National Institute of Food and Agriculture Hatch-IDA01506 for funding this work. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 1 Z9 1 U1 7 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2016 VL 106 IS 8 BP 854 EP 860 DI 10.1094/PHYTO-01-16-0026-R PG 7 WC Plant Sciences SC Plant Sciences GA DS5DS UT WOS:000380801600007 PM 27111802 ER PT J AU Ben-David, R Parks, R Dinoor, A Kosman, E Wicker, T Keller, B Cowger, C AF Ben-David, Roi Parks, Ryan Dinoor, Amos Kosman, Evsey Wicker, Thomas Keller, Beat Cowger, Christina TI Differentiation Among Blumeria graminis f. sp tritici Isolates Originating from Wild Versus Domesticated Triticum Species in Israel SO PHYTOPATHOLOGY LA English DT Article DE Triticum turgidum subsp dicoccoides; virulence analysis tool ID POWDERY MILDEW POPULATION; ERYSIPHE-GRAMINIS; LEAF RUST; WHEAT; DIVERSITY; VIRULENCE; INDIVIDUALS; CEREALS; HORDEI; GENOME AB Israel and its vicinity constitute a center of diversity of domesticated wheat species (Triticum aestivum and T. durum) and their sympatrically growing wild relatives, including wild emmer wheat (T. dicoccoides). We investigated differentiation within the forma specialis of their obligate powdery mildew pathogen, Blumeria graminis f. sp. tritici. A total of 61 B. graminis f. sp. tritici isolates were collected from the three host species in four geographic regions of Israel. Genetic relatedness of the isolates was characterized using both virulence patterns on 38 wheat lines (including 21 resistance gene differentials) and presumptively neutral molecular markers (simple-sequence repeats and single-nucleotide polymorphisms). All isolates were virulent on at least some genotypes of all three wheat species tested. All assays divided the B. graminis f. sp. tritici collection into two distinct groups, those from domesticated hosts and those from wild emmer wheat. One-way migration was detected from the domestic wheat B. graminis f. sp. tritici population to the wild emmer B. graminis f. sp. tritici population at a rate of five to six migrants per generation. This gene flow may help explain the overlap between the distinct domestic and wild B. graminis f. sp. tritici groups. Overall, B. graminis f. sp. tritici is significantly differentiated into wild-emmer and domesticated-wheat populations, although the results do not support the existence of a separate f. sp. dicocci. C1 [Ben-David, Roi] ARO Volcani Ctr, Inst Plant Sci, Dept Vegetables & Field Crops, IL-5025000 Bet Dagan, Israel. [Parks, Ryan; Cowger, Christina] North Carolina State Univ, USDA ARS, Dept Plant Pathol, Raleigh, NC 27695 USA. [Dinoor, Amos] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Dept Plant Pathol & Microbiol, IL-7610001 Rehovot, Israel. [Kosman, Evsey] Tel Aviv Univ, ICCI, George S Wise Fac Life Sci, IL-69978 Tel Aviv, Israel. [Wicker, Thomas; Keller, Beat] Univ Zurich, Dept Plant & Microbial Biol, Zollikerstr 107, CH-8008 Zurich, Switzerland. RP Ben-David, R (reprint author), ARO Volcani Ctr, Inst Plant Sci, Dept Vegetables & Field Crops, IL-5025000 Bet Dagan, Israel. EM roib@volcani.agri.gov.il FU University Research Priority Program Systems Biology/Functional Genomics grants; University of Zurich; European Research Council [ERC-2009-AdG 249996]; United States Department of Agriculture-Agricultural Research Service FX This work was supported by University Research Priority Program Systems Biology/Functional Genomics grants, University of Zurich, an Advanced Investigator Grant from the European Research Council (ERC-2009-AdG 249996, Durable resistance) and by the United States Department of Agriculture-Agricultural Research Service. NR 39 TC 0 Z9 0 U1 5 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2016 VL 106 IS 8 BP 861 EP 870 DI 10.1094/PHYTO-07-15-0177-R PG 10 WC Plant Sciences SC Plant Sciences GA DS5DS UT WOS:000380801600008 PM 27019062 ER PT J AU Scheuermann, KK Jia, YL AF Scheuermann, Klaus Konrad Jia, Yulin TI Identification of a Pi9-Containing Rice Germplasm with a Newly Developed Robust Marker SO PHYTOPATHOLOGY LA English DT Article ID BLAST RESISTANCE GENE; PI-TA GENE; MAGNAPORTHE-ORYZAE; PYRICULARIA-GRISEA; NATURAL VARIATION; CULTIVATED RICE; CORE COLLECTION; UNITED-STATES; LOCUS; DIVERSITY AB The Pi9 gene in rice, originating from Oryza minuta, is an effective resistance gene for controlling rice blast disease. However, currently available linked DNA markers do not accurately identify the function of Pi9, thus hindering its efficient incorporation into new cultivars through marker-assisted selection (MAS). In addition, no known Pi9-containing rice germplasm is available to breeders. In the present study, DNA sequence variation of Pi9 alleles and their family members was analyzed in 40 diverse rice germplasm accessions from the AA genome to develop a robust Pi9 marker. In total, 29 DNA primers of 20 to 23 nucleotides were designed and each possible combination of primer pairs was used to detect Pi9. Only one combination of DNA primers, KS28/KS6, was identified to specifically detect Pi9 in the monogenic line IRBL9-W. The presence of Pi9 was verified with the predicted Pi9-specific blast reaction. Subsequently, 201 genetically diverse mini-core rice accessions from 114 countries were screened with KS28/KS6. One germplasm, IR 9660-48-1-1-2, was identified to carry Pi9 and the function of Pi9 was verified with pathogenicity assays. This robust Pi9 marker and a rice germplasm, IR9660-48-1-1-2 (GSOR310687), carrying Pi9 can be used to improve blast resistance with a MAS approach. C1 [Scheuermann, Klaus Konrad] Santa Catarina State Agr Res & Rural Extens Agcy, Epagri EEI, Itajai Expt Stn, CP 277, BR-88318112 Itajai, SC, Brazil. [Jia, Yulin] USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 HWY 130 E, Stuttgart, AR 72160 USA. RP Jia, YL (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 HWY 130 E, Stuttgart, AR 72160 USA. EM yulin.jia@ars.usda.gov FU Brazilian National Council for Scientific and Technological Development Program Science Without Borders [248456/2013-0]; Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2013-68004-20378] FX We thank the USDA-ARS DB NRRC, Genetic Stocks Oryza (GSOR) Collection, Stuttgart, AR, for providing the rice seed; and T. Bianco, M. Lin, L. Bernhardt, and M. H. Jia of USDA-ARS DB NRRC for excellent technical support. This work was supported, in part, by the Brazilian National Council for Scientific and Technological Development Program Science Without Borders that conferred a Postdoctoral Scholarship on K. K. Scheuermann (248456/2013-0), and was recommended by scientific advisors of the Agriculture and Food Research Initiative Competitive Grant number 2013-68004-20378 from the USDA National Institute of Food and Agriculture. United States Department of Agriculture is an equal opportunity provider and employer. NR 45 TC 1 Z9 1 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2016 VL 106 IS 8 BP 871 EP 876 DI 10.1094/PHYTO-02-16-0091-R PG 6 WC Plant Sciences SC Plant Sciences GA DS5DS UT WOS:000380801600009 PM 27050577 ER PT J AU Burbank, LP Stenger, DC AF Burbank, Lindsey P. Stenger, Drake C. TI Plasmid Vectors for Xylella fastidiosa Utilizing a Toxin-Antitoxin System for Stability in the Absence of Antibiotic Selection SO PHYTOPATHOLOGY LA English DT Article ID VITIS-VINIFERA GRAPEVINES; POLYMERASE-CHAIN-REACTION; LEAF SCORCH DISEASE; PIERCES-DISEASE; STABLE MAINTENANCE; VIRULENCE; PEMK; PATHOGENICITY; COLONIZATION; RESISTANCE AB The phytopathogen Xylella fastidiosa causes disease in a variety of important crop and landscape plants. Functional genetic studies have led to a broader understanding of virulence mechanisms used by this pathogen in the grapevine host. Plasmid shuttle vectors are important tools in studies of bacterial genetics but there are only a limited number of plasmid vectors available that replicate in X. fastidiosa, and even fewer that are retained without antibiotic selection. Two plasmids are described here that show stable replication in X. fastidiosa and are effective for gene complementation both in vitro and in planta. Plasmid maintenance is facilitated by incorporation of the PemI/PemK plasmid addiction system, consisting of PemK, an endoribonuclease toxin, and its cognate antitoxin, PemI. Vector pXf20pemIK utilizes a native X. fastidiosa replication origin as well as a high-copy-number pUC origin for propagation in Escherichia coli cloning strains. Broad-host-range vector pBBR5pemIK is a medium- to low-copy-number plasmid based on the pBBR1 backbone. Both plasmids are maintained for extended periods of time in the absence of antibiotic selection, as well as up to 14 weeks in grapevine, without affecting bacterial fitness. These plasmids present an alternative to traditional complementation and expression vectors which rely on antibiotic selection for plasmid retention. C1 [Burbank, Lindsey P.; Stenger, Drake C.] USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. RP Burbank, LP (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM lindsey.burbank@ars.usda.gov FU USDA-ARS appropriated project [2034-22000-010-00D] FX We thank K. Zhang for technical support and M. S. Sisterson for statistical analysis. Funding for this work was from USDA-ARS appropriated project 2034-22000-010-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not constitute endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 44 TC 0 Z9 0 U1 13 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD AUG PY 2016 VL 106 IS 8 BP 928 EP 936 DI 10.1094/PHYTO-02-16-0097-R PG 9 WC Plant Sciences SC Plant Sciences GA DS5DS UT WOS:000380801600016 PM 27088393 ER PT J AU Garcia, RA Nieman, CM Haylock, RA Rosentrater, KA Piazza, GJ AF Garcia, R. A. Nieman, C. M. Haylock, R. A. Rosentrater, K. A. Piazza, G. J. TI The effect of chicken blood and its components on wastewater characteristics and sewage surcharges SO POULTRY SCIENCE LA English DT Article DE wastewater; blood; sewage surcharge; chicken; by-product ID SLAUGHTERHOUSE WASTE; POULTRY; FLOCCULANT AB Local wastewater treatment authorities levy surcharges from their non-residential customers that are based, in part, on the concentration of various pollutants in the customer's wastewater. Blood has long been recognized as the most potent contributor to pollutant loads in chicken processing plant wastewater. Quantification of the impact of blood on wastewater characteristics and sewage surcharges is hindered by lack of information on specific characteristics of chicken blood, and by the highly variable methods used by local authorities for calculating surcharges. In this study, the most commonly used wastewater characteristics are determined for whole chicken blood as well as its individual components. The characteristics measured include biochemical oxygen demand, chemical oxygen demand, total suspended solids, fats oil and grease, total Kjeldahl nitrogen, ammonia, and total phosphorus. Sewage surcharge calculation methods were collected from 71 local wastewater authorities. The results show all components of the blood to be extremely high-strength pollutants. The impact of blood on sewage surcharges is shown to be highly variable depending on the rates applied by the local authority. C1 [Garcia, R. A.; Nieman, C. M.; Piazza, G. J.] USDA ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. [Haylock, R. A.; Rosentrater, K. A.] Iowa State Univ, Dept Agr & Biosyst Engn, 1340 Elings Hall, Ames, IA 50011 USA. RP Garcia, RA (reprint author), USDA ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM rafael.garcia@ars.usda.gov NR 19 TC 0 Z9 0 U1 5 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2016 VL 95 IS 8 BP 1950 EP 1956 DI 10.3382/ps/pew114 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA DS5KT UT WOS:000380821600030 PM 27038423 ER PT J AU Bowker, B Zhuang, H AF Bowker, B. Zhuang, H. TI Impact of white striping on functionality attributes of broiler breast meat SO POULTRY SCIENCE LA English DT Article DE breast meat; meat quality; protein functionality; water-holding capacity; white striping ID PECTORALIS MAJOR MUSCLE; COMMERCIAL CONDITIONS; QUALITY TRAITS; FILLETS; CHICKENS; PROTEINS; PH AB The influence of white striping (WS) on the water-holding capacity (WHC) and protein functionality attributes of broiler breast meat was investigated. Boneless breast fillets (Pectoralis major) were collected from the deboning line of a commercial processing plant and categorized by WS score (normal, moderate, severe). The physical (weight, pH, CIE-color values), water-holding capacity (salt-induced water uptake, cook loss, final yield), protein functionality (solubility, emulsifying activity), and protein composition (SDS-PAGE) characteristics of the fillets were measured in three experiments. Breast meat with WS exhibited greater fillet weights, higher pH, and similar color values (L*a*b*) to normal fillets. In experiment 1, fillets were frozen-thawed prior to analysis. The WS condition reduced thaw loss, sarcoplasmic protein solubility, and the emulsifying activity of the myofibrillar proteins, but did not significantly affect salt-induced water uptake, cook loss, final yield, or myofibrillar protein solubility. In experiment 2, breast meat was analyzed fresh and after a freeze-thaw cycle. Freezing samples prior to analysis negatively influenced WHC and reduced sarcoplasmic protein solubility in both WS and normal fillets. In fresh and frozen-thawed meat, the WS condition decreased sarcoplasmic protein solubility but did not significantly alter WHC or myofibrillar protein solubility. For experiment 3, fillets were portioned into 3 sections (Location A, cranial end-ventral surface; Location B, cranial end-dorsal surface; Location C, caudal end). The effects of WS on WHC and protein solubility were dependent upon breast fillet sampling location. Fillets with WS exhibited lower salt-induced water uptake, greater cook loss, and lower sarcoplasmic protein solubility than normal fillets when sampled from location A. SDS-PAGE analysis indicated that differences in the composition of sarcoplasmic and myofibrillar protein fractions between WS and normal fillets were influenced by sampling location. These results suggest that WS diminishes the WHC and protein functionality of broiler breast meat, but demonstrate that the WS effects on these traits are not uniform throughout the breast muscle. C1 [Bowker, B.; Zhuang, H.] US Natl Poultry Res Ctr, USDA ARS, Athens, GA 30605 USA. RP Bowker, B (reprint author), US Natl Poultry Res Ctr, USDA ARS, Athens, GA 30605 USA. EM brian.bowker@ars.usda.gov NR 20 TC 0 Z9 0 U1 7 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2016 VL 95 IS 8 BP 1957 EP 1965 DI 10.3382/ps/pew115 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA DS5KT UT WOS:000380821600031 PM 27190107 ER PT J AU Baranowski, T Chen, TA O'Connor, TM Hughes, SO Diep, CS Beltran, A Brand, L Nicklas, T Baranowski, J AF Baranowski, Tom Chen, Tzu-An O'Connor, Teresia M. Hughes, Sheryl O. Diep, Cassandra S. Beltran, Alicia Brand, Leah Nicklas, Theresa Baranowski, Janice TI Predicting habits of vegetable parenting practices to facilitate the design of change programmes SO PUBLIC HEALTH NUTRITION LA English DT Article DE Parenting practices; Habit; Vegetables; Model of Goal Directed Behavior; Emotion ID GOAL-DIRECTED BEHAVIOR; FRUIT; MODEL; INTERVENTION; CONSUMPTION; PREVENTION; CHILDREN; MOTIVES AB Objective: Habit has been defined as the automatic performance of a usual behaviour. The present paper reports the relationships of variables from a Model of Goal Directed Behavior to four scales in regard to parents' habits when feeding their children: habit of (i) actively involving child in selection of vegetables; (ii) maintaining a positive vegetable environment; (iii) positive communications about vegetables; and (iv) controlling vegetable practices. We tested the hypothesis that the primary predictor of each habit variable would be the measure of the corresponding parenting practice. Design: Internet survey data from a mostly female sample. Primary analyses employed regression modelling with backward deletion, controlling for demo-graphics and parenting practices behaviour. Setting: Houston, Texas, USA. Subjects: Parents of 307 pre-school (3-5-year-old) children. Results: Three of the four models accounted for about 50 % of the variance in the parenting practices habit scales. Each habit scale was primarily predicted by the corresponding parenting practices scale (suggesting validity). The habit of active child involvement in vegetable selection was also most strongly predicted by two barriers and rudimentary self-efficacy; the habit of maintaining a positive vegetable environment by one barrier; the habit of maintaining positive communications about vegetables by an emotional scale; and the habit of controlling vegetable practices by a perceived behavioural control scale. Conclusions: The predictiveness of the psychosocial variables beyond parenting practices behaviour was modest. Discontinuing the habit of ineffective controlling parenting practices may require increasing the parent's perceived control of parenting practices, perhaps through simulated parent-child interactions. C1 [Baranowski, Tom; Chen, Tzu-An; O'Connor, Teresia M.; Hughes, Sheryl O.; Diep, Cassandra S.; Beltran, Alicia; Brand, Leah; Nicklas, Theresa; Baranowski, Janice] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. RP Baranowski, T (reprint author), USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu FU National Institute of Child Health and Human Development [HD075521] FX This research was funded by the National Institute of Child Health and Human Development (grant number HD075521) and institutional support from the US Department of Agriculture, Agricultural Research Service (Cooperative Agreement number 58-6250-0-008). The funders had no role in the design, analysis or writing of this article. NR 27 TC 0 Z9 0 U1 4 U2 4 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD AUG PY 2016 VL 19 IS 11 BP 1976 EP 1982 DI 10.1017/S1368980015003432 PG 7 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA DS6MY UT WOS:000380897300007 PM 26634349 ER PT J AU Thomson, JL Tussing-Humphreys, LM Zoellner, JM Goodman, MH AF Thomson, Jessica L. Tussing-Humphreys, Lisa M. Zoellner, Jamie M. Goodman, Melissa H. TI Psychosocial constructs were not mediators of intervention effects for dietary and physical activity outcomes in a church-based lifestyle intervention: Delta Body and Soul III SO PUBLIC HEALTH NUTRITION LA English DT Article DE Nutrition education; Supervised physical activity; Psychosocial constructs; Mediation; African American ID AFRICAN-AMERICAN ADULTS; SOCIAL SUPPORT; VEGETABLE INTAKE; SELF-EFFICACY; EFFECTIVENESS TRIAL; HEALTH BEHAVIOR; FRUIT; NUTRITION; PROGRAM; ASSOCIATIONS AB Objective: Evaluating an intervention's theoretical basis can inform design modifications to produce more effective interventions. Hence the present study's purpose was to determine if effects from a multicomponent lifestyle intervention were mediated by changes in the psychosocial constructs decisional balance, self-efficacy and social support. Design: Delta Body and Soul III, conducted from August 2011 to May 2012, was a 6-month, church-based, lifestyle intervention designed to improve diet quality and increase physical activity. Primary outcomes, diet quality and aerobic and strength/flexibility physical activity, as well as psychosocial constructs, were assessed via self-report, interviewer-administered surveys at baseline and post intervention. Mediation analyses were conducted using ordinary least squares (continuous outcomes) and maximum likelihood logistic (dichotomous outcomes) regression path analysis. Setting: Churches (five intervention and three control) were recruited from four counties in the Lower Mississippi Delta region of the USA. Subjects: Rural, Southern, primarily African-American adults (n 321). Results: Based upon results from the multiple mediation models, there was no evidence that treatment (intervention v. control) indirectly influenced changes in diet quality or physical activity through its effects on decisional balance, self-efficacy and social support. However, there was evidence for direct effects of social support for exercise on physical activity and of self-efficacy for sugar-sweetened beverages on diet quality. Conclusions: Results do not support the hypothesis that the psychosocial constructs decisional balance, self-efficacy and social support were the theoretical mechanisms by which the Delta Body and Soul III intervention influenced changes in diet quality and physical activity. C1 [Thomson, Jessica L.; Tussing-Humphreys, Lisa M.; Goodman, Melissa H.] ARS, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Dept Med, Chicago, IL USA. [Tussing-Humphreys, Lisa M.] Univ Illinois, Ctr Canc, Chicago, IL USA. [Zoellner, Jamie M.] Virginia Tech, Dept Human Nutr Foods & Exercise, Blacksburg, VA USA. RP Thomson, JL (reprint author), ARS, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM jessica.thomson@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service [6401-51000-001-00D]; US Department of Health and Human Services, Health Resources Services Administration [6 U1FRHA07411] FX This work was supported by the US Department of Agriculture, Agricultural Research Service (project number 6401-51000-001-00D); and the US Department of Health and Human Services, Health Resources Services Administration (grant number 6 U1FRHA07411). The views expressed are solely those of the authors and do not reflect the official policy or position of the US government. NR 40 TC 0 Z9 0 U1 4 U2 4 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD AUG PY 2016 VL 19 IS 11 BP 2060 EP 2069 DI 10.1017/S1368980015003602 PG 10 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA DS6MY UT WOS:000380897300016 PM 26797387 ER PT J AU Cook, D Gardner, DR Lee, ST Pfister, JA Stonecipher, CA Welsh, SL AF Cook, Daniel Gardner, Dale R. Lee, Stephen T. Pfister, James A. Stonecipher, Clinton A. Welsh, Stanley L. TI A swainsonine survey of North American Astragalus and Oxytropis taxa implicated as locoweeds SO TOXICON LA English DT Article DE Astragalus; Oxytropis; Locoweed; Swainsonine; Herbarium ID FUNGAL ENDOPHYTES; ALKALOID SWAINSONINE; EMBELLISIA-OXYTROPIS; ALPHA-MANNOSIDASE; UNDIFILUM; CANESCENS; LIVESTOCK; FABACEAE; SERICEA; CHINA AB Swainsonine, an indolizidine alkaloid with significant physiological activity, is an alpha-mannosidase and mannosidase II inhibitor that causes lysosomal storage disease and alters glycoprotein processing. Swainsonine is found in a number of plant species worldwide, and causes severe toxicosis in livestock grazing these plants, leading to a chronic wasting disease characterized by weight loss, depression, altered behavior, decreased libido, infertility, and death. Swainsonine has been detected in 19 Astragalus and 2 Oxytropis species in North America by thin layer chromatography, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and a jack bean alpha-mannosidase inhibition assay. In addition, 5 species in North America are presumed to contain swainsonine based upon reports from field cases. Many of these plant species have not been analyzed for swainsonine using modern instrumentation such as gas or liquid chromatography coupled with mass spectrometry. To provide clarification, 22 Astragalus species representing 93 taxa and 4 Oxytropis species representing 18 taxa were screened for swainsonine using both liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Swainsonine was detected in 48 Astragalus taxa representing 13 species and 5 Oxytropis taxa representing 4 species. Forty of the fifty-three swainsonine-positive taxa had not been determined to contain swainsonine previously using liquid or gas chromatography coupled with mass spectrometry. The list of swainsonine-containing taxa reported here will serve as a reference for risk assessment and diagnostic purposes. Published by Elsevier Ltd. C1 [Cook, Daniel; Gardner, Dale R.; Lee, Stephen T.; Pfister, James A.; Stonecipher, Clinton A.] USDA ARS, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. [Welsh, Stanley L.] Brigham Young Univ, Provo, UT 84602 USA. RP Cook, D (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 East 1400 North, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov NR 43 TC 0 Z9 0 U1 5 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD AUG PY 2016 VL 118 BP 104 EP 111 DI 10.1016/j.toxicon.2016.04.033 PG 8 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA DS2JB UT WOS:000380594700015 PM 27085305 ER PT J AU Son, K Tague, C Hunsaker, C AF Son, Kyongho Tague, Christina Hunsaker, Carolyn TI Effects of Model Spatial Resolution on Ecohydrologic Predictions and Their Sensitivity to Inter-Annual Climate Variability SO WATER LA English DT Article DE DEM resolution; ecohydrologic modeling; climate change effects; RHESSys; California's Sierra ID SNOW ACCUMULATION; GRID-SIZE; MOUNTAINOUS TERRAIN; ELEVATION MODELS; COMPLEX TERRAIN; SIERRA-NEVADA; CALIFORNIA; RUNOFF; EVAPOTRANSPIRATION; VEGETATION AB The effect of fine-scale topographic variability on model estimates of ecohydrologic responses to climate variability in California's Sierra Nevada watersheds has not been adequately quantified and may be important for supporting reliable climate-impact assessments. This study tested the effect of digital elevation model ( DEM) resolution on model accuracy and estimates of the sensitivity of ecohydrologic responses to inter-annual climate variability. The Regional Hydro-Ecologic Simulation System ( RHESSys) was applied to eight headwater, high-elevation watersheds located in the Kings River drainage basin. Each watershed was calibrated with measured snow depth ( or snow water equivalent) and daily streamflow. Modeled streamflow estimates were sensitive to DEM resolution, even with resolution-specific calibration of soil drainage parameters. For model resolutions coarser than 10 m, the accuracy of streamflow estimates largely decreased. Reduced model accuracy was related to the reduction in spatial variance of a topographic wetness index with coarser DEM resolutions. This study also found that among the long-term average ecohydrologic estimates, summer flow estimates were the most sensitive to DEM resolution, and coarser resolution models overestimated the climatic sensitivity for evapotranspiration and net primary productivity. Therefore, accounting for fine-scale topographic variability in ecohydrologic modeling may be necessary for reliably assessing climate change effects on lower-order Sierra Nevada watersheds (<= 2.3 km(2)). C1 [Son, Kyongho] CUNY, Res Fdn, New York, NY 10036 USA. [Tague, Christina] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93117 USA. [Hunsaker, Carolyn] US Forest Serv, Pacific Southwest Res Stn, USDA, Fresno, CA 93710 USA. RP Son, K (reprint author), CUNY, Res Fdn, New York, NY 10036 USA. EM kkyong77@hotmail.com; ctague@bren.ucsb.edu; chunsaker@fs.fed.us OI Hunsaker, Carolyn/0000-0002-5662-2402 FU National Science Foundation [EAR-0725097] FX We gratefully acknowledge the data collected by Kings River Experimental Watershed Project (KREW) and this study was financially supported by Southern Sierra Critical Zone Observatory Project from National Science Foundation (EAR-0725097). NR 37 TC 0 Z9 0 U1 6 U2 6 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 2073-4441 J9 WATER-SUI JI Water PD AUG PY 2016 VL 8 IS 8 AR 321 DI 10.3390/w8080321 PG 20 WC Water Resources SC Water Resources GA DS4QG UT WOS:000380765200010 ER PT J AU Lee, H Kim, MS Lim, HS Park, E Lee, WH Cho, BK AF Lee, Hoonsoo Kim, Moon S. Lim, Hyoun-Sub Park, Eunsoo Lee, Wang-Hee Cho, Byoung-Kwan TI Detection of cucumber green mottle mosaic virus-infected watermelon seeds using a near-infrared (NIR) hyperspectral imaging system: Application to seeds of the "Sambok Honey" cultivar SO BIOSYSTEMS ENGINEERING LA English DT Article DE Cucumber green mottle mosaic virus (CGMMV); Watermelon seeds; Near-infrared (NIR) hyperspectral imaging; Partial least square discriminant analysis (PLS-DA); Least square support vector machine (LS-SVM) ID SPECTROSCOPY; QUALITY; PHENOLICS; DISCRIMINATION; IDENTIFICATION; PREDICTION; CAPACITY; TOBACCO; SAFETY; PLANTS AB The cucurbit diseases caused by cucumber green mottle mosaic virus (CGMMV) have led to a serious problem to growers and seed producers because it is difficult to prevent spreading through pathogen-infected seeds. Conventional detection methods for infected seeds such as biological, serological, and molecular measurements are not practical for measuring entire samples due to their destructive nature, and time, and cost issues. For this reason, it is necessary to develop a rapid and non-destructive novel technique for detecting seeds infestation. A near-infrared (NIR) hyperspectral imaging system was used to discriminate virus-infected seeds from healthy seeds with partial least square discriminant analysis (PLS-DA) and least square support vector machine (LS-SVM). The classification accuracy for virus-infected watermelon seeds were 83.3% with the best model, demonstrating the potential of NIR hyperspectral imaging for detection of virus-infected watermelon seeds. (C) 2016 IAgrE. Published by Elsevier Ltd. All rights reserved. C1 [Lee, Hoonsoo; Park, Eunsoo; Lee, Wang-Hee; Cho, Byoung-Kwan] Chungnam Natl Univ, Coll Agr & Life Sci, Dept Biosyst Machinery Engn, 99 Daehak Ro, Daejeon 305764, South Korea. [Kim, Moon S.] ARS, Environm Microbiol & Food Safety Lab, USDA, BARC East, Powder Mill Rd Bldg 303, Beltsville, MD 20705 USA. [Lim, Hyoun-Sub] Chungnam Natl Univ, Coll Agr & Life Sci, Dept Appl Biol, 99 Daehak Ro, Daejeon 305764, South Korea. RP Cho, BK (reprint author), Chungnam Natl Univ, Coll Agr & Life Sci, Dept Biosyst Machinery Engn, 99 Daehak Ro, Daejeon 305764, South Korea. EM hoonsoolee83@gmail.com; moon.kim@ars.usda.gov; hyounlim@cnu.ac.kr; besoo0407@gmail.com; wanghee@cnu.ac.kr; chobk@cnu.ac.kr FU Technology Commercialization Support Program; Ministry of Agriculture, Food and Rural Affairs (MAFRA); Next Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ01125602] FX This research was partially supported by Technology Commercialization Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA) and by a grant from the Next Generation BioGreen 21 Program (No. PJ01125602), Rural Development Administration, Republic of Korea. NR 42 TC 1 Z9 1 U1 20 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 EI 1537-5129 J9 BIOSYST ENG JI Biosyst. Eng. PD AUG PY 2016 VL 148 BP 138 EP 147 DI 10.1016/j.biosystemseng.2016.05.014 PG 10 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA DS2PU UT WOS:000380626800014 ER PT J AU Zhong, J Lin, ZY Ma, YM Gao, BD Liu, HQ Zhao, TC Schaad, NW AF Zhong, Jie Lin, Zhen-Ya Ma, Ya-Min Gao, Bi-Da Liu, Hui-Qin Zhao, Ting-Chang Schaad, Norman W. TI Rapid Discrimination between Groups I and II of Acidovorax citrulli Using a Primer Pair Specific to a pilL Gene SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE Acidovorax citrulli; group I(II); primers ID AVENAE SUBSP CITRULLI; WATERMELON FRUIT BLOTCH; SEED; NOV; CATTLEYAE AB Acidovorax citrulli can be divided into two genetic groups: group I and group II based primarily on pulsed-field gel electrophoresis (PFGE) and multilocus sequence classification (MLST). To distinguish more rapidly between strains of the two groups, a pair of specific primer for specific polymerase chain reaction (PCR) that can identify group II strains was designed based on the pilL gene of a group II strain, AAC00-1. PCR results showed that a 332-bp band was generated for 51 of 52 group II strains whereas only three of 93 group I strains were positive, largely consisting with previous studies of A.citrulli classification. Results of PCR showed the primers were able to detect group II strains of A.citrulli and distinguish between strains of groups I and II rapidly and accurately. C1 [Zhong, Jie; Lin, Zhen-Ya; Ma, Ya-Min; Gao, Bi-Da] Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China. [Liu, Hui-Qin; Zhao, Ting-Chang] Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China. [Schaad, Norman W.] USDA ARS, Foreign Dis Weed Sci Reserch Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. RP Gao, BD (reprint author), Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Nongda Rd 1, Changsha 410128, Hunan, Peoples R China.; Zhao, TC (reprint author), Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China. EM bdgao@aliyun.com; tczhao@ippcaas.cn FU Special Fund for Agro-Scientific Research in the Public Interest of China [201003066]; National Key Technology R&D Program of China [2012BAD15B04-1] FX This research was sponsored funded by the Special Fund for Agro-Scientific Research in the Public Interest of China (201003066) and The National Key Technology R&D Program of China (2012BAD15B04-1). We thank R.R. Walcott and Saul Burdman for their material support. NR 22 TC 1 Z9 1 U1 9 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-1785 EI 1439-0434 J9 J PHYTOPATHOL JI J. Phytopathol. PD AUG PY 2016 VL 164 IS 7-8 BP 558 EP 562 DI 10.1111/jph.12435 PG 5 WC Plant Sciences SC Plant Sciences GA DS1HR UT WOS:000380347000015 ER PT J AU Chen, Y Stark, NM Tshabalala, MA Gao, JM Fan, YM AF Chen, Yao Stark, Nicole M. Tshabalala, Mandla A. Gao, Jianmin Fan, Yongming TI Weathering Characteristics of Wood Plastic Composites Reinforced with Extracted or Delignified Wood Flour SO Materials LA English DT Article DE WPCs; weathering; degradation; delignification; extractives ID DENSITY POLYETHYLENE COMPOSITES; SURFACE-CHEMISTRY CHANGES; POLYMER COMPOSITES; FTIR SPECTROSCOPY; DURABILITY; COLOR; FILMS; XPS AB This study investigated weathering performance of an HDPE wood plastic composite reinforced with extracted or delignified wood flour (WF). The wood flour was pre-extracted with three different solvents, toluene/ethanol (TE), acetone/water (AW), and hot water (HW), or sodium chlorite/acetic acid. The spectral properties of the composites before and after artificial weathering under accelerated conditions were characterized by Fourier transform infrared (FTIR) spectroscopy, the surface color parameters were analyzed using colorimetry, and the mechanical properties were determined by a flexural test. Weathering of WPC resulted in a surface lightening and a decrease in wood index (wood/HDPE) and flexural strength. WPCs that were reinforced with delignified wood flour showed higher Delta L* and Delta E* values, together with lower MOE and MOR retention ratios upon weathering when compared to those with non-extracted control and extracted WF. C1 [Chen, Yao; Gao, Jianmin; Fan, Yongming] Beijing Forestry Univ, MOE Key Lab Wooden Mat Sci & Applicat, Beijing 100083, Peoples R China. [Stark, Nicole M.; Tshabalala, Mandla A.] US Forest Serv, USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Chen, Y (reprint author), Beijing Forestry Univ, MOE Key Lab Wooden Mat Sci & Applicat, Beijing 100083, Peoples R China. EM ychen@bjfu.edu.cn; nstark@fs.fed.us; mtshabalala@fs.fed.us; gaojm@bjfu.edu.cn; fanym@bjfu.edu.cn FU National Natural Science Foundation of China [31400498]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China [14JIX-01] FX Financial support from the National Natural Science Foundation of China (series number of the project 31400498) is gratefully acknowledged. This research was funded in part by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China (Grant No. 14JIX-01). The authors express their sincere thanks to Sara J. Fishwild at USDA Forest Products Laboratory for the flexural test. NR 35 TC 0 Z9 0 U1 12 U2 19 PU MDPI AG PI BASEL PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND SN 1996-1944 J9 MATERIALS JI Materials PD AUG PY 2016 VL 9 IS 8 AR 610 DI 10.3390/ma9080610 PG 12 WC Materials Science, Multidisciplinary SC Materials Science GA DS4PC UT WOS:000380762000001 ER PT J AU Rudgers, JA Fletcher, RA Olivas, E Young, CA Charlton, ND Pearson, DE Maron, J AF Rudgers, Jennifer A. Fletcher, Rebecca A. Olivas, Eric Young, Carolyn A. Charlton, Nikki D. Pearson, Dean E. Maron, John L. TI Long-term ungulate exclusion reduces fungal symbiont prevalence in native grasslands SO OECOLOGIA LA English DT Article DE Epichloe; Epidemiology; Festuca; Herbivory; Mutualism ID TALL FESCUE; VERTICAL TRANSMISSION; ENDOPHYTE INFECTION; LOLIUM-PERENNE; EPICHLOE; GRASSES; POPULATIONS; HERBIVORES; TOLERANCE; DIVERSITY AB When symbionts are inherited by offspring, they can have substantial ecological and evolutionary consequences because they occur in all host life stages. Although natural frequencies of inherited symbionts are commonly < 100 %, few studies investigate the ecological drivers of variation in symbiont prevalence. In plants, inherited fungal endophytes can improve resistance to herbivory, growth under drought, and competitive ability. We evaluated whether native ungulate herbivory increased the prevalence of a fungal endophyte in the common, native bunchgrass, Festuca campestris (rough fescue, Poaceae). We used large-scale (1 ha) and long-term (7-10 year) fencing treatments to exclude native ungulates and recorded shifts in endophyte prevalence at the scale of plant populations and for individual plants. We characterized the fungal endophyte in F. campestris, EpichloA << species FcaTG-1 (F. campestris taxonomic group 1) for the first time. Under ungulate exclusion, endophyte prevalence was 19 % lower in plant populations, 25 % lower within plant individuals, and 39 % lower in offspring (seeds) than in ungulate-exposed controls. Population-level endophyte frequencies were also negatively correlated with soil moisture across geographic sites. Observations of high within-plant variability in symbiont prevalence are novel for the EpichloA << species, and contribute to a small, but growing, literature that documents phenotypic plasticity in plant-endophyte symbiota. Altogether, we show that native ungulates can be an important driver of symbiont prevalence in native plant populations, even in the absence of evidence for direct mechanisms of mammal deterrence. Understanding the ecological controls on symbiont prevalence could help to predict future shifts in grasslands that are dominated by EpichloA << host plants. C1 [Rudgers, Jennifer A.; Olivas, Eric] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Fletcher, Rebecca A.; Pearson, Dean E.; Maron, John L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Young, Carolyn A.; Charlton, Nikki D.] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. [Pearson, Dean E.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59812 USA. RP Rudgers, JA (reprint author), Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. EM jrudgers@unm.edu OI Young, Carolyn/0000-0003-0406-8398 FU US National Science Foundation [DEB-0614406, DEB-1354972, 1145588] FX J. M. and D. E. P. were supported by the US National Science Foundation DEB-0614406. J. A. R. was supported by the US National Science Foundation DEB-1354972 and 1145588. The authors have no other conflicts of interest to declare. Thanks to Beth Haley and Ben Goodheart for field and laboratory assistance. This article does not contain any studies with human participants or animals performed by any of the authors. NR 61 TC 1 Z9 1 U1 7 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD AUG PY 2016 VL 181 IS 4 BP 1151 EP 1161 DI 10.1007/s00442-016-3620-7 PG 11 WC Ecology SC Environmental Sciences & Ecology GA DS0JR UT WOS:000380282100017 PM 27113054 ER PT J AU Weaver, CM Dawson-Hughes, B Lappe, JM Wallace, TC AF Weaver, C. M. Dawson-Hughes, B. Lappe, J. M. Wallace, T. C. TI Re: Errors in the NOF meta-analysis of calcium and vitamin D supplements SO OSTEOPOROSIS INTERNATIONAL LA English DT Letter ID UPDATED METAANALYSIS; HIP-FRACTURES; ELDERLY-WOMEN C1 [Weaver, C. M.] Purdue Univ, Dept Nutr Sci, Womens Global Hlth Inst, W Lafayette, IN 47907 USA. [Dawson-Hughes, B.] Tufts Univ, Bone Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lappe, J. M.] Creighton Univ, Sch Nursing, Omaha, NE 68178 USA. [Lappe, J. M.] Creighton Univ, Sch Med, Omaha, NE USA. [Wallace, T. C.] Natl Osteoporosis Fdn, Washington, DC 20036 USA. [Wallace, T. C.] George Mason Univ, Dept Nutr & Food Studies, Fairfax, VA 22030 USA. [Wallace, T. C.] Natl Osteoporosis Fdn, 251 18th St S Suite 630, Arlington, VA 22202 USA. RP Wallace, TC (reprint author), Natl Osteoporosis Fdn, Washington, DC 20036 USA.; Wallace, TC (reprint author), George Mason Univ, Dept Nutr & Food Studies, Fairfax, VA 22030 USA.; Wallace, TC (reprint author), Natl Osteoporosis Fdn, 251 18th St S Suite 630, Arlington, VA 22202 USA. EM weavercm@purdue.edu; bess.dawson-hughes@tufts.edu; joanlappe@creighton.edu; taylor.wallace@nof.org NR 9 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD AUG PY 2016 VL 27 IS 8 BP 2641 EP 2642 DI 10.1007/s00198-016-3525-7 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DR7YE UT WOS:000380114800028 PM 26992925 ER PT J AU Weaver, CM Dawson-Hughes, B Lappe, JM Wallace, TC AF Weaver, C. M. Dawson-Hughes, B. Lappe, J. M. Wallace, T. C. TI Calcium plus vitamin D supplementation and the risk of fractures: an updated meta-analysis from the National Osteoporosis Foundation (vol 27, pg 367, 2016) SO OSTEOPOROSIS INTERNATIONAL LA English DT Correction ID CONTROLLED-TRIAL; ELDERLY-WOMEN; HIP FRACTURE; PREVENTION C1 [Weaver, C. M.] Purdue Univ, Dept Nutr Sci, Womens Global Hlth Inst, W Lafayette, IN 47907 USA. [Dawson-Hughes, B.] Tufts Univ, Bone Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lappe, J. M.] Creighton Univ, Sch Nursing, Omaha, NE 68178 USA. [Lappe, J. M.] Creighton Univ, Sch Med, Omaha, NE USA. [Wallace, T. C.] Natl Osteoporosis Fdn, Washington, DC 20036 USA. [Wallace, T. C.] George Mason Univ, Dept Nutr & Food Studies, Fairfax, VA 22030 USA. [Wallace, T. C.] Natl Osteoporosis Fdn, 251 18th St S Suite 630, Arlington, VA 22202 USA. RP Wallace, TC (reprint author), Natl Osteoporosis Fdn, Washington, DC 20036 USA.; Wallace, TC (reprint author), George Mason Univ, Dept Nutr & Food Studies, Fairfax, VA 22030 USA.; Wallace, TC (reprint author), Natl Osteoporosis Fdn, 251 18th St S Suite 630, Arlington, VA 22202 USA. EM weavercm@purdue.edu; bess.dawson-hughes@tufts.edu; joanlappe@creighton.edu; taylor.wallace@me.com NR 10 TC 1 Z9 1 U1 7 U2 8 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD AUG PY 2016 VL 27 IS 8 BP 2643 EP 2646 DI 10.1007/s00198-016-3699-z PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA DR7YE UT WOS:000380114800029 ER PT J AU Wasserstrom, H Whitford, WG Steinberger, Y AF Wasserstrom, Haggai Whitford, Walter G. Steinberger, Yosef TI Spatiotemporal Variations of Soil Microarthropod Communities in the Negev Desert SO PEDOSPHERE LA English DT Article DE microhabitat; seasonality; shrub; soil depth; soil mites; soil moisture; soil organic carbon ID ZYGOPHYLLUM-DUMOSUM; CHIHUAHUAN DESERT; VERTICAL-DISTRIBUTION; MICROBIAL-POPULATION; FOOD WEBS; PATTERNS; DECOMPOSITION; ECOSYSTEM; DYNAMICS; CANOPY AB Desert ecosystems are characterized by sparse vegetation that affects both abiotic parameters and soil biota along the soil profile. This study was conducted in 2010-2011 in a loess plain in the northern Negev Desert highlands, Israel, to test two main hypotheses: 1) the abundance and diversity of microarthropods would vary seasonally in the top 30-cm soil layer, but would be relatively stable at soil depths between 30 and 50 cm and 2) soil microarthropods would be more abundant in soils under shrubs with large litter accumulations than under shrubs with less litter or bare soil. Soil samples were collected each season from the 0-50 cm profile at 10-cm intervals under the canopies of Hammada scoparia and Zygophyllum, dumosum and from the bare interspaces between them. Soil moisture and soil organic carbon in the top 30-cm layers varied seasonally, but there was little variation in the soil layers deeper than 30 cm. Soil mites were most abundant in the top 30-cm soil layer in autumn and winter, with the highest number of families found in winter. There were no differences in soil microarthropod abundance attributable to the presence or absence of shrubs of either species. The microarthropod communities of the microhabitats studied consisted of Acari, Psocoptera, and Collembola. The Acari were mostly identified to the family level and were dominated by Oribatida (55%) and Prostigmata (41%) in all seasons and microhabitats, while the psocopterans were most abundant in summer. These results are opposite to those obtained in other studies in similar xeric environments. Moreover, our findings were not in line with our hypothesis that a better microhabitat played a major role in microarthropod community composition, diversity, and density. C1 [Wasserstrom, Haggai; Steinberger, Yosef] Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-5290002 Ramat Gan, Israel. [Whitford, Walter G.] New Mexico State Univ, USDA ARS Jornada Expt Range, MSC 3JER, Las Cruces, NM 88003 USA. RP Steinberger, Y (reprint author), Bar Ilan Univ, Mina & Everard Goodman Fac Life Sci, IL-5290002 Ramat Gan, Israel. EM yosef.steinberger@biu.ac.il NR 49 TC 1 Z9 1 U1 8 U2 8 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD AUG PY 2016 VL 26 IS 4 BP 451 EP 461 DI 10.1016/S1002-0160(15)60056-X PG 11 WC Soil Science SC Agriculture GA DS0PO UT WOS:000380299400003 ER PT J AU Smits, KM Kirby, E Massman, WJ Baggett, LS AF Smits, Kathleen M. Kirby, Elizabeth Massman, William J. Baggett, Larry Scott TI Experimental and Modeling Study of Forest Fire Effect on Soil Thermal Conductivity SO PEDOSPHERE LA English DT Article DE controlled burn; degree of saturation; empirical model; water content; wildfire ID WATER-CONTENT; ORGANIC-MATTER; HEAT; TEMPERATURE; MOISTURE; DENSITY; REPELLENCY; MINERALOGY; CAPACITY; POROSITY AB An understanding of soil thermal conductivity after a wildfire or controlled burn is important to land management and post-fire recovery efforts. Although soil thermal conductivity has been well studied for non-fire heated soils, comprehensive data that evaluate the long-term effect of extreme heating from a fire on the soil thermal conductivity are limited. The purpose of this study was to evaluate the long-term impact of fire on the effective thermal conductivity of soils by directly comparing fire-heated and no-fire control soils through a series of laboratory studies. The thermal conductivity was measured for ten soil samples from two sites within the Manitou Experimental Forest, Colorado, USA, for a range of water contents from saturation to the residual degree of saturation. The thermal conductivity measured was compared with independent estimates made using three empirical models from literature, including the Campbell et al. (1994), Cote and Konrad (2005), and Massman et al. (2008) models. Results demonstrate that for the test soils studied, the thermal conductivity of the fire-heated sails was slightly lower than that of the control soils for all observed water contents. Modeling results show that the Campbell et al. (1994) model gave the best agreement over the full range of water contents when proper fitting parameters were employed. Further studies are needed to evaluate the significance of including the influence of fire burn on the thermal properties of soils in modeling studies. C1 [Smits, Kathleen M.; Kirby, Elizabeth] Colorado Sch Mines, Dept Civil & Environm Engn, Ctr Expt Study Subsurface Environm Proc, Golden, CO 80401 USA. [Massman, William J.; Baggett, Larry Scott] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80256 USA. RP Smits, KM (reprint author), Colorado Sch Mines, Dept Civil & Environm Engn, Ctr Expt Study Subsurface Environm Proc, Golden, CO 80401 USA. EM ksmits@mines.edu FU National Science Foundation (NSF), USA [DGE-0638719] FX This research was supported by the National Science Foundation (NSF), USA (division of graduate education, No. DGE-0638719). The authors would like to thank Tiande (Jack) Wu, Colorado School of Mines, USA, for experimental assistance. NR 65 TC 0 Z9 0 U1 10 U2 10 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD AUG PY 2016 VL 26 IS 4 BP 462 EP 473 DI 10.1016/S1002-0160(15)60057-1 PG 12 WC Soil Science SC Agriculture GA DS0PO UT WOS:000380299400004 ER PT J AU Bahder, BW Zalom, FG Sudarshana, MR AF Bahder, Brian W. Zalom, Frank G. Sudarshana, Mysore R. TI An Evaluation of the Flora Adjacent to Wine Grape Vineyards for the Presence of Alternative Host Plants of Grapevine red blotch-associated virus SO PLANT DISEASE LA English DT Article ID 1ST REPORT; GEMINIVIRUS; CALIFORNIA; DISTANCE AB Grapevine red blotch-associated virus (GRBaV) is a recently discovered virus of concern to wine grape production in North America. While the vector of this virus is unknown, other elements of virus epidemiology are essential to develop guidelines for the management of the virus as well as to assist in the search for its vector. The objective of this study was to evaluate vegetation within and surrounding GRBaV-infected vineyards to identify potential virus reservoirs that may serve as sources of inoculum. In this study, 13 plant species were sampled throughout the year and were tested for the presence of GRBaV. Of the 13 species tested, two species, Rubus amzeniacus and wild grapes (Vitis californica x V. vinifera), tested positive by quantitative PCR. Of these two species, only wild grapes were determined to be a true host. This study documents the first time GRBaV has been confirmed in an alternative host or in a species outside of a commercial vineyard and suggests that a mechanism exists by which GRBaV moves between plant species that is not human-mediated. The precise role that wild grapes play in the epidemiology of GRBaV remains unknown. C1 [Bahder, Brian W.; Zalom, Frank G.] Univ Calif Davis, Dept Entomol & Nematol, 1 Shields Ave, Davis, CA 95616 USA. [Sudarshana, Mysore R.] Univ Calif Davis, Dept Plant Pathol, USDA ARS, 1 Shields Ave, Davis, CA 95616 USA. RP Sudarshana, MR (reprint author), Univ Calif Davis, Dept Plant Pathol, USDA ARS, 1 Shields Ave, Davis, CA 95616 USA. EM mysore.sudarshana@ars.usda.gov FU USDA-ARS CRIS [5306-220000-014-00D]; California Grapevine Rootstock Improvement Commission; California Tree Fruit and Nut Crops and Grapevine Industry Board; California Department of Food and Agriculture's Specialty Crops Research and Improvement Block Grant Program FX This work was supported by USDA-ARS CRIS 5306-220000-014-00D. Additional funding for this research was provided by grants from the California Grapevine Rootstock Improvement Commission, the California Tree Fruit and Nut Crops and Grapevine Industry Board, and the California Department of Food and Agriculture's Specialty Crops Research and Improvement Block Grant Program. The authors thank Michael Bollinger and Meredith Castillo, for assistance in sample collection and processing. NR 20 TC 2 Z9 2 U1 9 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1571 EP 1574 DI 10.1094/PDIS-02-16-0153-RE PG 4 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200007 ER PT J AU Rondon, SI Roster, MS Hamlin, LL Green, KJ Karasev, AV Crosslin, JM AF Rondon, Silvia I. Roster, Mary Sue Hamlin, Launa L. Green, Kelsie J. Karasev, Alexander V. Crosslin, J. M. TI Characterization of Beet curly top virus Strains Circulating in Beet Leafhoppers (Hemiptera: Cicadellidae) in Northeastern Oregon SO PLANT DISEASE LA English DT Article ID COLUMBIA BASIN; SEQUENCES; RECOMBINATION; PHYTOPLASMA; CALIFORNIA; PLANTS; WASHINGTON; INFECTION; ALIGNMENT; GENETICS AB The beet leafhopper, Circulifer tenellus, is an agriculturally important pest, particularly in the western United States. This insect transmits the Beet curly top virus (BCTV) to multiple crops, including bean, tomato, and pepper. In this study, we investigated the incidence of BCTV in individual leafhoppers collected at several sites in northeastern Oregon during the growing season in 2007, 2008, and 2009. Of the 800 insects tested, 151 (18.9%) were found positive for the virus. Percentage of virus incidence varied from 0% at one location in 2009 to a high of 55.6% for a location sampled in 2008. The complete virus genomes from one virus-positive insect collected in each of the 3 years were determined. BLAST analysis of the BCTV whole-genome sequences from 2007, 2008, and 2009 insects showed 98, 94, and 96% identities with the BCTV-Worland sequence (AY134867), respectively. The BCTV_2008 sequence showed the greatest identity (96%) with another BCTV genomic sequence (JN817383), and was found to be a recombinant between the BCTV-Worland type, representing the majority of the genome (approximately 2.2 kb), and the BCTV-CFH type that provided an approximately 0.8-kb fragment spanning replication-related genes Cl and C2. This area of the BCTV genome, between the Cl and C2 genes, was previously found to carry symptom determinants of the virus, and the data may suggest more severe effects of BCTV during the 2008 season. Results indicate that BCTV is common and widespread in C. tenellus in eastern Oregon and that there is substantial genetic diversity among the virus strains present in this important field and vegetable crop-growing region. C1 [Rondon, Silvia I.] Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. [Roster, Mary Sue; Hamlin, Launa L.] USDA ARS, Prosser, WA 99350 USA. [Green, Kelsie J.; Karasev, Alexander V.; Crosslin, J. M.] Univ Idaho, Moscow, ID 83843 USA. RP Rondon, SI (reprint author), Oregon State Univ, Hermiston Agr Res & Extens Ctr, Hermiston, OR 97838 USA. EM silvia.rondon@oregonstate.edu FU Oregon Potato Commission; Idaho Bean Commission; Snake River Sugar Beet Research Alliance; Idaho Agricultural Experiment Station FX This work was funded, in part, through grants provided by the Oregon Potato Commission, Idaho Bean Commission, Snake River Sugar Beet Research Alliance, and the Idaho Agricultural Experiment Station. NR 33 TC 0 Z9 0 U1 5 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1586 EP 1590 DI 10.1094/PDIS-10-15-1189-RE PG 5 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200010 ER PT J AU Probst, C Nelson, ME Grove, GG Twomey, MC Gent, DH AF Probst, Claudia Nelson, Mark E. Grove, Gary G. Twomey, Megan C. Gent, David H. TI Hop Powdery Mildew Control Through Alteration of Spring Pruning Practices SO PLANT DISEASE LA English DT Article ID DOWNY MILDEW; MANAGEMENT AB Podosphaera macularis, the causal agent of hop powdery mildew, is a recurrent threat to hops in the Pacific Northwest because of the potential to reduce cone yield and quality. Early-season pruning is a common practice in hop production for horticultural reasons. Studies were conducted over a 3-year period in a commercial hop yard to quantify the effect of pruning method and timing on disease development, yield, and cone quality factors. A 4-week delay in pruning reduced the incidence of leaves with powdery mildew from 46 to 10% and cones from 9 to 1%, with the specific effect being season dependent. Pruning using chemical desiccants rather than by mechanical means had similar effects on disease levels on leaves. On cones, though, chemical pruning had a small but significant reduction in the incidence of powdery mildew compared with mechanical pruning. Cone yield, levels of bittering-acids, and color were not negatively affected in any individual year or cumulatively over three seasons when pruning treatments were applied repeatedly to the same plots during the study period. Delayed pruning may offer a low-cost means of reducing both the incidence of powdery mildew and early season fungicide inputs in certain cultivars. C1 [Probst, Claudia; Nelson, Mark E.; Grove, Gary G.] Washington State Univ, Dept Plant Pathol, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Twomey, Megan C.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gent, David H.] Oregon State Univ, Forage Seed & Cereal Res Unit, USDA ARS, Corvallis, OR 97331 USA. [Gent, David H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Gent, DH (reprint author), Oregon State Univ, Forage Seed & Cereal Res Unit, USDA ARS, Corvallis, OR 97331 USA.; Gent, DH (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU Washington State University Agriculture Research Center; Hop Research Council; Washington Hop Commission; Washington State Commission on Pesticide Registration; United States Department of Agriculture-Agricultural Research Service Current Research Information System [5358-21000-040-00]; Washington State Department of Agriculture Specialty Crops Block Grant program FX Financial support for this research was provided by Washington State University Agriculture Research Center, Hop Research Council, Washington Hop Commission, Washington State Commission on Pesticide Registration, United States Department of Agriculture-Agricultural Research Service Current Research Information System 5358-21000-040-00, and the Washington State Department of Agriculture Specialty Crops Block Grant program. NR 24 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1599 EP 1605 DI 10.1094/PDIS-10-15-1127-RE PG 7 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200012 ER PT J AU Krugner, R Ledbetter, CA AF Krugner, R. Ledbetter, C. A. TI Rootstock Effects on Almond Leaf Scorch Disease Incidence and Severity SO PLANT DISEASE LA English DT Article ID XYLELLA-FASTIDIOSA; PIERCES DISEASE; PHONY DISEASE; CALIFORNIA; PEACH; TRANSMISSION; GRAPEVINES; HYBRID; GROWTH; PLUM AB A 5-year field study was conducted to evaluate effects of duration and exclusion of Xylella fastidiosa infections on young almond tree performance and their links to tree vigor. 'Nemaguard,' Okinawa, 'Nonpareil,' and Y119 were used as rootstocks for almond scion 'Sonora.' Among X. fastidiosa-infected trees, there was significant etiological heterogeneity with i) absence of leaf scorching symptoms in the presence of reduced growth, presence of leaf scorching symptoms in the absence of reduced growth, and ii) severe leaf scorching and reduced growth. Trunk cross sectional areas of X. fastidiosa-infected trees grafted on 'Nemaguard' and 'Nonpareil' rootstocks were significantly smaller than noninfected trees, whereas trunk size of trees grafted on 'Okinawa' and Y119 was not affected by infection status. Severity of leaf scorching symptoms was highest on trees grafted on 'Nonpareil' rootstock, intermediate on 'Okinawa' and Y119, and lowest on Vemaguard.' X. fastidiosa infections and seasonal leaf scorching symptoms persisted on most inoculated trees throughout the study, except on trees grafted on 'Nemaguard' that manifested complete leaf scorching symptom remission and apparent elimination of the pathogen after the second year. Results indicate that depending on rootstock type X. fastidiosa can affect trunk size in a relatively short period and/or persist for years as trees grow. C1 [Krugner, R.; Ledbetter, C. A.] USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. RP Krugner, R (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM Rodrigo.Krugner@ars.usda.gov FU USDA-ARS [2034-22000-010-00D] FX We thank Theresa de la Torre, Aaron J. Salyers, Matthew Escoto, and Melissa Fujioka for technical assistance. This work was funded by USDA-ARS appropriated project # 2034-22000-010-00D. NR 34 TC 0 Z9 0 U1 6 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1617 EP 1621 DI 10.1094/PDIS-01-16-0125-RE PG 5 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200014 ER PT J AU Guo, YH Olsen, RT Kramer, M Pooler, M AF Guo, Yonghong Olsen, Richard T. Kramer, Matthew Pooler, Margaret TI Use of Mycelium and Detached Leaves in Bioassays for Assessing Resistance to Boxwood Blight SO PLANT DISEASE LA English DT Article ID CALONECTRIA-PSEUDONAVICULATA; 1ST REPORT; CYLINDROCLADIUM-PSEUDONAVICULATUM; SHEATH BLIGHT; BUXICOLA; INOCULATION; DISEASE; BUXUS AB Boxwood blight caused by Calonectria pseudonaviculata is a newly emergent disease of boxwood (Buxus spp. L.) in the United States that causes leaf drop, stem lesions, and plant death. A rapid and reliable laboratory assay that enables screening hundreds of boxwood genotypes for resistance to boxwood blight is needed to enable breeding and selection of resistant cultivars. Using eight boxwood cultivars with differing susceptibilities, we examined parameters for a screening assay comparing whole plant inoculation with detached leaf inoculation, use of mycelium versus spores as the inoculum, comparison of times of the year for inoculation, and comparison of two leaf inoculation methods. Inoculation of detached leaves gave comparable results to inoculation of whole plants when compared across genotypes, although the detached leaf assay resulted in greater percentages of symptom expression. The time of year of plant inoculation (spring, summer, or winter) did not affect the relative expression of symptoms among the most resistant and susceptible genotypes. Inoculating plants with mycelium was as effective as spore inoculation for causing disease symptoms and allowed us to distinguish the more resistant genotypes, yet mycelium inoculation was much easier to prepare in large quantities for multiple assays. C1 [Guo, Yonghong; Olsen, Richard T.; Pooler, Margaret] ARS, USDA, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD 20705 USA. [Kramer, Matthew] ARS, USDA, Stat Grp, Beltsville, MD 20705 USA. RP Pooler, M (reprint author), ARS, USDA, US Natl Arboretum Floral & Nursery Plants Res Uni, Beltsville, MD 20705 USA. EM margaret.pooler@ars.usda.gov NR 22 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1622 EP 1626 DI 10.1094/PDIS-01-16-0016-RE PG 5 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200015 ER PT J AU Kolmer, JA Hughes, ME AF Kolmer, J. A. Hughes, M. E. TI Physiologic Specialization of Puccinia triticina on Wheat in the United States in 2014 SO PLANT DISEASE LA English DT Article ID F-SP TRITICI; LEAF RUST RESISTANCE; NORTH-AMERICA; SPRING WHEAT; VIRULENCE; POPULATIONS; CULTIVARS; DIVERSITY; CANADA; GENES AB Collections of Puccinia triticina obtained from wheat fields and breeding plots in the Great Plains, Ohio River Valley, and southeastern states, were tested for virulence in 2014 in order to determine the virulence of the wheat leaf rust pathogen population in the United States. Single uredinial isolates (380 total) were derived from the collections and tested for virulence phenotype on 20 lines of Thatcher wheat that are near-isogenic for leaf rust resistance genes. In 2014, 55 virulence phenotypes were described in the United States. Virulence phenotypes MBTNB, TBBGS, and TCRKG were the three most common phenotypes. Phenotypes MBTNB and TCRKG are both virulent to Lr11, and TCRKG is also virulent to Lr18 and Lr26. MBTNB and TCRKG were most common in the soft red winter wheat region of the southeastern states and the Ohio Valley. Phenotype TBBGS is virulent to Lr39, which is present in the hard red winter wheat cultivars, and Lr21, which is present in the hard red spring wheat cultivars. Isolates with virulence to Lr11, Lr18, and Lr26 were most common in the southeastern states and Ohio Valley region. Isolates with virulence to Lr21 and Lr39 were most common in the hard red wheat region of the southern and northern Great Plains. C1 [Kolmer, J. A.; Hughes, M. E.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Kolmer, JA (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM jim.kolmer@ars.usda.gov NR 20 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1768 EP 1773 DI 10.1094/PDIS-12-15-1461-SR PG 6 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200032 ER PT J AU Costanzo, S Rascoe, J Zhao, Y Davis, RE Nakhla, MK AF Costanzo, S. Rascoe, J. Zhao, Y. Davis, R. E. Nakhla, M. K. TI First Report of a New 'Candidatus Phytoplasma pini'-Related Strain Associated with Witches'-Broom of Pinus spp. in Maryland SO PLANT DISEASE LA English DT News Item C1 [Costanzo, S.; Rascoe, J.; Nakhla, M. K.] USDA APHIS PPQ CPHST, Beltsville, MD 20705 USA. [Zhao, Y.; Davis, R. E.] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Costanzo, S (reprint author), USDA APHIS PPQ CPHST, Beltsville, MD 20705 USA. NR 5 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1776 EP 1776 DI 10.1094/PDIS-01-16-0097-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200038 ER PT J AU Bao, YX Sun, HJ Li, YF Duan, ZZ McCord, PH Cui, YP Zhang, MQ AF Bao, Y. X. Sun, H. J. Li, Y. F. Duan, Z. Z. McCord, P. H. Cui, Y. P. Zhang, M. Q. TI First Report of Fusarium oxysporum Isolate gx3 Causing Sugarcane Pokkah Boeng in Guangxi of China. SO PLANT DISEASE LA English DT News Item C1 [Bao, Y. X.; Sun, H. J.; Li, Y. F.; Duan, Z. Z.; Cui, Y. P.; Zhang, M. Q.] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agribi, Nanning 530005, Peoples R China. [McCord, P. H.] ARS, USDA, Sugarcane Field Stn, Canal Point, FL 33438 USA. RP Bao, YX (reprint author), Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agribi, Nanning 530005, Peoples R China. NR 4 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1785 EP 1785 DI 10.1094/PDIS-01-16-0080-PDN PG 1 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200061 ER PT J AU Ceron-Bustamante, M Ayala-Escobar, V Nava-Diaz, C Ward, TJ Leyva-Mir, G Villasenor-Mir, HE AF Ceron-Bustamante, M. Ayala-Escobar, V. Nava-Diaz, C. Ward, T. J. Leyva-Mir, G. Villasenor-Mir, H. E. TI First Report of Fusarium meridionale Causing Fusarium Head Blight of Wheat in Mexico. SO PLANT DISEASE LA English DT News Item ID POPULATIONS; GRAMINEARUM C1 [Ceron-Bustamante, M.; Ayala-Escobar, V.; Nava-Diaz, C.] Colegio Postgrad, Inst Fitosanidad, Carretera Mexico Texcoco Km 36-5, Texcoco 56230, Mexico. [Ward, T. J.] ARS, Mycotoxin Prevent & Appl Microbiol Res Unit, USDA, Peoria, IL 61604 USA. [Leyva-Mir, G.] Univ Autonoma Chapingo, Dept Parasitol Agr, Carretera Mexico Texcoco Km 38-5, Texcoco 56230, Mexico. [Villasenor-Mir, H. E.] Campo Expt Valle Mexico CEVAMEX, Inst Invest Forestales & Agr INIFAP, Carretera Mexico Texcoco Km 13-5, Texcoco 56230, Mexico. RP Ceron-Bustamante, M (reprint author), Colegio Postgrad, Inst Fitosanidad, Carretera Mexico Texcoco Km 36-5, Texcoco 56230, Mexico. NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1790 EP 1791 DI 10.1094/PDIS-11-15-1315-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200075 ER PT J AU Weiland, JE Sniezko, RA Wiseman, MS Serdani, M Putnam, ML AF Weiland, J. E. Sniezko, R. A. Wiseman, M. S. Serdani, M. Putnam, M. L. TI First Report of Phaeobotryon cupressi Causing Canker of Calocedrus decurrens (Incense-Cedar) in Oregon. SO PLANT DISEASE LA English DT News Item C1 [Weiland, J. E.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Sniezko, R. A.] Forest Serv, USDA, Dorena Genet Resource Ctr, Cottage Grove, OR 97424 USA. [Wiseman, M. S.; Serdani, M.; Putnam, M. L.] Oregon State Univ, Bot & Plant Pathol Dept, Corvallis, OR 97331 USA. RP Weiland, JE (reprint author), ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. NR 2 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1793 EP 1793 PG 1 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200081 ER PT J AU Yan, GP Plaisance, A Huang, D Liu, Z Chapara, V Handoo, ZA AF Yan, G. P. Plaisance, A. Huang, D. Liu, Z. Chapara, V. Handoo, Z. A. TI First Report of the Root-lesion Nematode Pratylenchus neglectus on Wheat (Triticum aestivum) in North Dakota. SO PLANT DISEASE LA English DT News Item ID SOIL C1 [Yan, G. P.; Plaisance, A.; Huang, D.; Liu, Z.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Chapara, V.] NDSU, Langdon Res Extens Ctr, Langdon, ND 58249 USA. [Handoo, Z. A.] USDA ARS, Nematol Lab, Beltsville, MD 20705 USA. RP Yan, GP (reprint author), North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. NR 5 TC 1 Z9 1 U1 6 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1794 EP 1795 DI 10.1094/PDIS-02-16-0260-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200085 ER PT J AU Adegbola, RO Fulmer, AM Williams, B Brenneman, TB Kemerait, RC Sheard, W Woodward, JE Adkins, S Naidu, RA AF Adegbola, R. O. Fulmer, A. M. Williams, B. Brenneman, T. B. Kemerait, R. C. Sheard, W. Woodward, J. E. Adkins, S. Naidu, R. A. TI First Report of the Natural Occurrence of Tomato chlorotie spot virus in Peanuts in Haiti. SO PLANT DISEASE LA English DT News Item ID TOSPOVIRUSES; FLORIDA C1 [Adegbola, R. O.; Naidu, R. A.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA. [Sheard, W.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31794 USA. [Woodward, J. E.] Meds & Foods Kids, Cap Haitien, Haiti. [Adkins, S.] Texas A&M Univ Res & Extens Ctr, Dept Plant Pathol & Microbiol, Lubbock, TX 79403 USA. USDA ARS, Ft Pierce, FL 34945 USA. RP Adegbola, RO (reprint author), Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD AUG PY 2016 VL 100 IS 8 BP 1797 EP 1798 DI 10.1094/PDIS-01-16-0070-PDN PG 2 WC Plant Sciences SC Plant Sciences GA DS0PM UT WOS:000380299200093 ER PT J AU Edwards, JV Mao, NT Russell, S Carus, E Condon, B Hinchliffe, D Gary, L Graves, E Bopp, A Wang, YY AF Edwards, J. Vincent Mao, Ningtao Russell, Stephen Carus, Edmund Condon, Brian Hinchliffe, Doug Gary, Lawson Graves, Elena Bopp, Alvin Wang, Yiyi TI Fluid handling and fabric handle profiles of hydroentangled greige cotton and spunbond polypropylene nonwoven topsheets SO PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART L-JOURNAL OF MATERIALS-DESIGN AND APPLICATIONS LA English DT Article DE Fluid handling; fabric handle; greige cotton; nonwoven; topsheet ID DISPOSABLE DIAPERS; HUMAN SKIN; WATER; FRICTION; ABSORBENCY; ANISOTROPY; HYDRATION; TEXTILES; PRESSURE; PRODUCTS AB Wettable nonwoven topsheets are traditionally spunbond polypropylene nonwoven fabrics. The fluid handling performance of hydroentangled greige cotton nonwovens was studied to determine their suitability for topsheet applications based upon analysis of fluid rewet, strikethrough, and acquisition properties; and the relative contributions of nonwoven cotton's cellulosic and wax components to hydrophobic and hydrophilic fluid transport properties are addressed. It was observed that mechanically cleaned greige cotton nonwovens exhibit certain fluid handling properties that are similar to polypropylene spunbond-meltblown topsheets, partly as a result of the residual wax content. Subsequently, the surface polarity, swelling, and moisture uptake of 100% greige cotton and 50:50 blends of greige cotton and polypropylene hydroentangled nonwovens were studied in comparison with the performance of a commercially available 100% polypropylene spunbond-meltblown topsheets. The surface polarity, swelling, and wettability values obtained from electrokinetic and water contact angle analysis were found to be in agreement with the hydrophobic polypropylene topsheets. Additionally, comfort assessment was undertaken based upon fabric handle profiles using the Leeds University Fabric Handle Evaluation System, which is an objective evaluation based on the quantification of fabric buckling deformations. Of the fabrics studied in this work, 50:50 greige cotton/polypropylene hydroentangled fabrics were the softest as determined by the Leeds University Fabric Handle Evaluation System and exhibited fluid handling properties consistent with the requirements of commercial topsheets. C1 [Edwards, J. Vincent; Condon, Brian; Hinchliffe, Doug; Graves, Elena] Southern Reg Res Ctr, New Orleans, LA 70124 USA. [Mao, Ningtao; Russell, Stephen; Wang, Yiyi] Univ Leeds, Performance Text & Clothing Res Grp, Leeds LS2 9JT, W Yorkshire, England. [Carus, Edmund; Gary, Lawson] TJ Beall Co, Drew, MS USA. [Bopp, Alvin] Southern Univ, New Orleans, LA USA. RP Edwards, JV (reprint author), USDA, Southern Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM vince.edwards@ars.usda.gov NR 50 TC 0 Z9 0 U1 6 U2 6 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1464-4207 EI 2041-3076 J9 P I MECH ENG L-J MAT JI Proc. Inst. Mech. Eng. Pt. L-J. Mater.-Design Appl. PD AUG PY 2016 VL 230 IS 4 BP 847 EP 859 DI 10.1177/1464420715586020 PG 13 WC Materials Science, Multidisciplinary SC Materials Science GA DS1EI UT WOS:000380338200002 ER PT J AU Hanberry, BB Nowacki, GJ AF Hanberry, Brice B. Nowacki, Gregory J. TI Oaks were the historical foundation genus of the east-central United States SO QUATERNARY SCIENCE REVIEWS LA English DT Article DE American chestnut; Eastern deciduous forest; Hickories; Mesophication; Pines; Quercus ID NORTH-AMERICA; FOREST ECOSYSTEMS; DYNAMICS; FIRE; CONSERVATION; TREES; QUERCUS; USA; RESTORATION; HYPOTHESIS AB Foundation tree species are dominant and define ecosystems. Because of the historical importance of oaks (Quercus) in east-central United States, it was unlikely that oak associates, such as pines (Pinus), hickories (Carya) and chestnut (Castanea), rose to this status. We used 46 historical tree studies or databases (ca. 1620-1900) covering 28 states, 1.7 million trees, and 50% of the area of the eastern United States to examine importance of oaks compared to pines, hickories, and chestnuts. Oak was the most abundant genus, ranging from 40% to 70% of total tree composition at the ecological province scale and generally increasing in dominance from east to west across this area. Pines, hickories, and chestnuts were co-dominant (ratio of oak composition to other genera of <2) in no more than five of 70 ecological subsections and two of 20 ecological sections in east-central United States, and thus by definition, were not foundational. Although other genera may be called foundational because of localized abundance or perceptions resulting from inherited viewpoints, they decline from consideration when compared to overwhelming oak abundance across this spatial extent. The open structure and high-light conditions of oak ecosystems uniquely supported species-rich understories. Loss of oak as a foundation genus has occurred with loss of open forest ecosystems at landscape scales. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Hanberry, Brice B.] Univ Missouri, 203 Nat Resources Bldg, Columbia, MO 65211 USA. [Nowacki, Gregory J.] USDA Forest Serv, Eastern Reg Off, 626 E Wisconsin Ave, Milwaukee, WI 53202 USA. RP Hanberry, BB (reprint author), Univ Missouri, 203 Nat Resources Bldg, Columbia, MO 65211 USA. EM hanberryb@missouri.edu NR 67 TC 1 Z9 1 U1 14 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0277-3791 J9 QUATERNARY SCI REV JI Quat. Sci. Rev. PD AUG 1 PY 2016 VL 145 BP 94 EP 103 DI 10.1016/j.quascirev.2016.05.037 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DS2MD UT WOS:000380602700005 ER PT J AU Chen, NS Zhu, JY Tong, ZH AF Chen, Nusheng Zhu, J. Y. Tong, Zhaohui TI Fabrication of microfibrillated cellulose gel from waste pulp sludge via mild maceration combined with mechanical shearing SO CELLULOSE LA English DT Article DE Microfibrillated cellulose (MFC); Disc refining; Ultrasonication plus homogenization; Storage modulus ID HIGH-PRESSURE HOMOGENIZATION; ENDOGLUCANASE TREATMENT; RHEOLOGICAL PROPERTIES; NATIVE CELLULOSE; ACID-HYDROLYSIS; WOOD CELLULOSE; NANOFIBERS; WATER; MICROFLUIDIZATION; COMPOSITES AB This article describes a facile route, which combines mild maceration of waste pulp sludge and a mechanical shearing process, to prepare microfibrillated cellulose (MFC) with a high storage modulus. In the maceration, the mixture of glacial acetic acid and hydrogen peroxide was used to extract cellulose from never-dried waste pulp sludge. Then, two different mechanical processes including disc refining (DR) and ultrasonication plus homogenization (UH) were applied to the cellulose after maceration and resulted in MFC with a highly tangled fibril network. All of the resultant cellulosic suspensions (2 % w/w) exhibited a gel-like and shear-thinning behavior with storage moduli (G') ranging from 200 to 4000 Pa. Among them, the 30-min DR-treated MFC gels had the maximum G', which was much higher than for previously reported MFC gels at the same concentration. Additionally, after mechanical processing, specific surface areas and water retention values of MFC were accordingly increased with the enhancement of shear force, while the storage moduli (G') were not consistently increased. Finally, a strong MFC gel was successfully prepared from never-dried waste pulp sludge via a one-step disc refining process and using cost-effective chemicals. The obtained hydrogels will have potential as low-density reinforcing fillers or as a template for further surface modification. C1 [Chen, Nusheng; Tong, Zhaohui] Univ Florida, Dept Agr & Biol Engn, POB 110570, Gainesville, FL 32611 USA. [Zhu, J. Y.] USDA, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. RP Tong, ZH (reprint author), Univ Florida, Dept Agr & Biol Engn, POB 110570, Gainesville, FL 32611 USA. EM ztong@ufl.edu NR 40 TC 0 Z9 0 U1 7 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD AUG PY 2016 VL 23 IS 4 BP 2573 EP 2583 DI 10.1007/s10570-016-0959-1 PG 11 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA DR7OJ UT WOS:000380089300024 ER PT J AU Hojsgaard, DH Burson, BL Quarin, CL Martinez, EJ AF Hojsgaard, D. H. Burson, B. L. Quarin, C. L. Martinez, E. J. TI Unravelling the ambiguous reproductive biology of Paspalum malacophyllum: a decades old story clarified SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Apomixis; Breeding programs; Embryology; Flow cytometry; Paspalum malacophyllum; Poaceae; Progeny test; Sexuality ID EMBRYO-SAC FORMATION; EVOLUTIONARY IMPLICATIONS; APOMIXIS; BAHIAGRASS; EXPRESSION; PATTERNS; PANICOIDEAE; ACCESSIONS; DIVERSITY; SEXUALITY AB In a recent manuscript published by our group we analyzed the reproductive biology of the grass Paspalum malacophyllum by using traditional embryological techniques combined with current cytological and molecular methods. Our findings confirmed apparent contradictions regarding the reproductive behavior of P. malacophyllum from six independently published reports over the past six decades. Herein we summarize the main findings, conclusions, and validations of all previous studies, highlighting the need for multiple approaches to characterize reproductive systems when using apomictic plants in a breeding program. C1 [Hojsgaard, D. H.] Univ Gottingen, Albrecht von Haller Inst Plant Sci, Dept Systemat Biodivers & Evolut Plants, Untere Karspule 2, D-37073 Gottingen, Germany. [Burson, B. L.] Texas A&M Univ, USDA ARS, Dept Soil & Crop Sci, College Stn, TX 77845 USA. [Quarin, C. L.; Martinez, E. J.] Univ Nacl Nordeste FCA UNNE, Inst Bot Nordeste IBONE, Fac Ciencias Agr, CC 209, RA-3400 Corrientes, Argentina. RP Hojsgaard, DH (reprint author), Univ Gottingen, Albrecht von Haller Inst Plant Sci, Dept Systemat Biodivers & Evolut Plants, Untere Karspule 2, D-37073 Gottingen, Germany. EM Diego.Hojsgaard@biologie.uni-goettingen.de FU German Research Foundation (DFG) [HO 5462/1-1]; National Council for Scientific and Technological Research (CONICET) of DFG-CONICET [20150202-167] FX We thank Professor Carlos Acuna, University of Northeast, Corrientes, Argentina, and two anonymous reviewers for their critical comments and suggestions on a preliminary version of the manuscript. This work was funded by the German Research Foundation (DFG Project HO 5462/1-1 to D.H.H.) and the National Council for Scientific and Technological Research (CONICET Project 20150202-167 to E.J.M.), in the framework of DFG-CONICET international cooperation. NR 56 TC 0 Z9 0 U1 5 U2 5 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD AUG PY 2016 VL 63 IS 6 BP 1063 EP 1071 DI 10.1007/s10722-015-0303-x PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA DR9QQ UT WOS:000380231600011 ER PT J AU von Rein, I Gessler, A Premke, K Keitel, C Ulrich, A Kayler, ZE AF von Rein, Isabell Gessler, Arthur Premke, Katrin Keitel, Claudia Ulrich, Andreas Kayler, Zachary E. TI Forest understory plant and soil microbial response to an experimentally induced drought and heat-pulse event: the importance of maintaining the continuum SO GLOBAL CHANGE BIOLOGY LA English DT Article DE (CO2)-C-13 pulse labeling; 16S rRNA next-generation sequencing; climate extremes; drought; forest understory; heat-pulse; microbial community structure; plant-soil-microbe carbon continuum; PLFAs ID CLIMATE-CHANGE; COMMUNITY COMPOSITION; TERRESTRIAL ECOSYSTEMS; TEMPERATE GRASSLAND; CARBON ALLOCATION; SUMMER DROUGHT; BOREAL FOREST; PEAT SOILS; BACTERIAL; RESILIENCE AB Drought duration and intensity are expected to increase with global climate change. How changes in water availability and temperature affect the combined plant-soil-microorganism response remains uncertain. We excavated soil monoliths from a beech (Fagus sylvatica L.) forest, thus keeping the understory plant-microbe communities intact, imposed an extreme climate event, consisting of drought and/or a single heat-pulse event, and followed microbial community dynamics over a time period of 28days. During the treatment, we labeled the canopy with (CO2)-C-13 with the goal of (i) determining the strength of plant-microbe carbon linkages under control, drought, heat and heat-drought treatments and (ii) characterizing microbial groups that are tightly linked to the plant-soil carbon continuum based on C-13-labeled PLFAs. Additionally, we used 16S rRNA sequencing of bacteria from the Ah horizon to determine the short-term changes in the active microbial community. The treatments did not sever within-plant transport over the experiment, and carbon sinks belowground were still active. Based on the relative distribution of labeled carbon to roots and microbial PLFAs, we determined that soil microbes appear to have a stronger carbon sink strength during environmental stress. High-throughput sequencing of the 16S rRNA revealed multiple trajectories in microbial community shifts within the different treatments. Heat in combination with drought had a clear negative effect on microbial diversity and resulted in a distinct shift in the microbial community structure that also corresponded to the lowest level of label found in the PLFAs. Hence, the strongest changes in microbial abundances occurred in the heat-drought treatment where plants were most severely affected. Our study suggests that many of the shifts in the microbial communities that we might expect from extreme environmental stress will result from the plant-soil-microbial dynamics rather than from direct effects of drought and heat on soil microbes alone. C1 [von Rein, Isabell; Gessler, Arthur; Premke, Katrin; Ulrich, Andreas; Kayler, Zachary E.] Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, D-15374 Muncheberg, Germany. [Gessler, Arthur] Berlin Brandenburg Inst Adv Biodivers Res BBIB, Altensteinstr 6, D-14195 Berlin, Germany. [Gessler, Arthur] Swiss Fed Inst Forest Snow & Landscape Res WSL, Zurcherstr 111, CH-8903 Birmensdorf, Switzerland. [Premke, Katrin] Leibniz Inst Freshwater Ecol & Inland Fisheries C, Muggelseedamm 310, D-12587 Berlin, Germany. [Keitel, Claudia] Univ Sydney, Fac Agr & Environm, Ctr Carbon Water & Food, 380 Werombi Rd, Brownlow Hill, NSW 2570, Australia. RP Ulrich, A (reprint author), Leibniz Ctr Agr Landscape Res ZALF, Inst Landscape Biogeochem, D-15374 Muncheberg, Germany.; Kayler, ZE (reprint author), USDA Forest Serv, Northern Res Stn, Lawrence Livermore Natl Lab, Livermore, CA 94550 USA. EM zkayler@fs.fed.us; zkayler@fs.fed.us RI Gessler, Arthur/C-7121-2008; Ulrich, Andreas/G-3956-2012 OI Gessler, Arthur/0000-0002-1910-9589; Ulrich, Andreas/0000-0003-3854-9608 NR 85 TC 3 Z9 3 U1 30 U2 53 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD AUG PY 2016 VL 22 IS 8 BP 2861 EP 2874 DI 10.1111/gcb.13270 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR6MU UT WOS:000380016800018 PM 26946456 ER PT J AU Sedej, I Milczarek, R Wang, SC Sheng, RQ Avena-Bustillos, RD Dao, L Takeoka, G AF Sedej, Ivana Milczarek, Rebecca Wang, Selina C. Sheng, Runqi Avena-Bustillos, Roberto de Jesus Dao, Lan Takeoka, Gary TI Spray drying of a phenolic-rich membrane filtration fraction of olive mill wastewater: optimisation and dried product quality SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Filtered olive mill wastewater; optimisation; phenolics; reverse osmosis retentate; spray drying ID PURIFIED HYDROXYTYROSOL; ANTIOXIDANT PROPERTIES; RECOVERY; POWDER; WASTEWATERS; BIOPHENOLS; EXTRACTS; SYSTEM; YIELD AB Olive mill wastewater (OMWW) from 3- and 2-phase mills was subjected to a two-step membrane filtration process. The obtained reverse osmosis retentate (RO-R) is a phenolic-rich coproduct stream, and the reverse osmosis permeate is a near-pure water stream. A pilot-scale spray dryer was used for the production of RO-R powder. The optimum temperature/feed pump conditions were obtained for 3-phase (150 degrees C/4.4Hz) and for 2-phase RO-R (175 degrees C/3.8Hz). The powders obtained by spray drying under these conditions were analysed for physicochemical properties, including identification and quantification of phenolic compounds. Protocatechuic acid, 3-hydroxytyrosol, tyrosol, vanillic acid and p-coumaric acid were quantified in both RO-R powders, which show the possibility to obtain a phenolic-rich powder from an OMWW filtration fraction. This will lead towards better usage of by-products from olive oil production. C1 [Sedej, Ivana; Wang, Selina C.; Sheng, Runqi] Univ Calif Davis, Olive Ctr, 392 Old Davis Rd, Davis, CA 95616 USA. [Milczarek, Rebecca; Avena-Bustillos, Roberto de Jesus; Dao, Lan; Takeoka, Gary] USDA ARS, Hlth Proc Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. RP Sedej, I (reprint author), Univ Calif Davis, Olive Ctr, 392 Old Davis Rd, Davis, CA 95616 USA. EM ivana.sedej@ars.usda.gov FU California Department of Food and Agriculture [SCB12040] FX This work is a part of the Specialty Crop Block Grant Program - SCB12040, supported by the California Department of Food and Agriculture. OMWW samples were generously donated by California Olive Ranch (Artois, CA, USA) and Seka Hills (Brooks, CA, USA). We are also grateful to New Logic Research, Inc. (Emeryville, CA, USA) for providing the filtration system and training of its operation. NR 33 TC 0 Z9 0 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD AUG PY 2016 VL 51 IS 8 BP 1900 EP 1909 DI 10.1111/ijfs.13163 PG 10 WC Food Science & Technology SC Food Science & Technology GA DR7GS UT WOS:000380068600019 ER PT J AU Porensky, LM Mueller, KE Augustine, DJ Derner, JD AF Porensky, Lauren M. Mueller, Kevin E. Augustine, David J. Derner, Justin D. TI Thresholds and gradients in a semi-arid grassland: long-term grazing treatments induce slow, continuous and reversible vegetation change SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE alternative stable states; blue grama; cool-season grasses; grazing intensity; Hesperostipa comata; non-equilibrium dynamics; Pascopyrum smithii; rangeland; state-and-transition models; stocking rates ID TRANSITION MODELS; STOCKING RATE; ECONOMIC RESPONSES; SHORTGRASS STEPPE; NORTH-AMERICA; PRAIRIE; RANGELANDS; COMMUNITY; CATTLE; STRATEGIES AB Temporal changes in semi-arid ecosystems can include transitions between alternative stable states, involving thresholds and multiple domains of attraction, but can also include relatively continuous, symmetric and reversible shifts within a single stable state. Conceptual state-and-transition models (STMs) describe both types of ecosystem dynamics by including state transitions (plant community changes difficult-to-reverse without substantial input or effort) and phase shifts (easily reversible community changes) as consequences of management practices and environmental variability. Grazing management is purported to be the primary driver of state transitions in current STMs for North American grasslands, but there is limited empirical evidence from these grasslands showing that grazing can cause difficult-to-reverse transitions between alternate stable states. In a northern mixed-grass prairie in Wyoming, USA, we examined plant community responses to (i) long-term (33-year) grazing intensity treatments (none, light, moderate and heavy stocking rates) and (ii) 8years of light or no grazing in pastures that were grazed heavily for the previous 25years. Long-term grazing treatments were associated with distinct, but not stable, plant communities. From year 22 to 33, heavier stocking rates decreased cover of dominant C-3 grasses and increased cover of the dominant C-4 grass Bouteloua gracilis. Reversing stocking rates from heavy to light or no grazing resulted in reversal of changes induced by prior heavy stocking for dominant C-3 grasses, but not for B.gracilis. For both groups, rates of change following grazing treatment reversals were consistent with rates of change during the initial years of the experiment (1982-1990).Synthesis and applications. In a semi-arid rangeland with a long evolutionary history of grazing, different long-term grazing intensity treatments caused slow, continuous and directional changes with important management implications, but did not appear to induce alternative stable states. For this and similar ecosystems, quantifying the time-scales and compositional gradients associated with key phase shifts may be more important than identifying thresholds between alternative stable states. C1 [Porensky, Lauren M.; Mueller, Kevin E.; Augustine, David J.] USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. [Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. RP Porensky, LM (reprint author), USDA ARS, Rangeland Resources Res Unit, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM lauren.porensky@ars.usda.gov FU USDA-ARS FX Thanks to Dr. Richard Hart for initiating this study and to the University of Wyoming and 8A Hay and Cattle Company for providing steers. Funding was from USDA-ARS. Matt Mortenson ably maintained the infrastructure. Matt, Jeff Thomas, Mary Ashby and others conducted vegetation sampling. NR 48 TC 1 Z9 1 U1 17 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD AUG PY 2016 VL 53 IS 4 BP 1013 EP 1022 DI 10.1111/1365-2664.12630 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7FO UT WOS:000380065600006 ER PT J AU Roberts, MW D'Amato, AW Kern, CC Palik, BJ AF Roberts, Margaret W. D'Amato, Anthony W. Kern, Christel C. Palik, Brian J. TI Long-term impacts of variable retention harvesting on ground-layer plant communities in Pinus resinosa forests SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE disturbance regimes; ecological forestry; herbaceous layer; recalcitrant understorey; silviculture; temperate forests ID GREEN-TREE RETENTION; NORTHERN HARDWOOD FORESTS; UNDERSTORY VEGETATION; SPECIES DIVERSITY; DECIDUOUS FORESTS; ECOLOGICAL FILTER; HERBACEOUS LAYER; FERN UNDERSTORY; BOREAL FORESTS; VARYING LEVELS AB Concerns about loss of biodiversity and structural complexity in managed forests have recently increased and led to the development of new management strategies focused on restoring or maintaining ecosystem functions while also providing wood outputs. Variable retention harvest (VRH) systems, in which mature overstorey trees are retained in various spatial arrangements across harvested areas, represent one potential approach to this problem. However, long-term evaluations of the effectiveness of this strategy at sustaining plant community composition are needed as this strategy is increasingly applied in managed forest landscapes throughout the world. The forest ground layer plays a central role in forest ecosystem functioning, and we evaluated the long-term (11+ year) dynamics in ground-layer plant communities in response to VRH study in Pinus resinosa Aiton. forests. This large-scale, manipulative study included four overstorey (control, small gap-aggregated, large gap-aggregated and dispersed) and two understorey (ambient and reduced shrubs) treatments replicated four times in 16-ha stands. Changes in ground-layer community composition were apparent 11years following harvest, regardless of live-tree retention pattern. Richness and diversity increased and were driven by introduction and colonization of ruderal species, while forest interior species continued to persist across treatments. All life-forms responded positively to harvest with the exception of moss and clubmoss species. The lack of effect of spatial pattern of retention on ground-layer plant communities was likely related to the presence of a dense and persistent shrub layer, a result of decades of fire suppression. In particular, the greatest responses to overstorey retention pattern occurred in areas receiving shrub reduction treatments, indicating this recalcitrant layer likely filtered response to retention pattern.Synthesis and applications. Overall, this work highlights flexibility in choosing a variable retention harvest approach when sustaining ground-layer plant community diversity and composition are goals, but altered disturbance regimes (e.g. fire suppression, timber harvesting) that have facilitated the presence or formation of recalcitrant understories, need to be considered. The legacy effects of historical land use and alterations to natural disturbance regimes on the understorey in northern temperate forests are of equal, if not greater, importance to overstorey retention patterns in eliciting desired responses to variable retention harvest and need to be more carefully considered in future applications of this method. C1 [Roberts, Margaret W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [D'Amato, Anthony W.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05095 USA. [Kern, Christel C.] US Forest Serv, Northern Res Stn, USDA, Rhinelander, WI 54501 USA. [Palik, Brian J.] US Forest Serv, Northern Res Stn, USDA, Grand Rapids, MN 55744 USA. RP Roberts, MW (reprint author), 311 Illick Hall,1 Forestry Dr, Syracuse, NY USA. EM mrobe101@syr.edu FU UMN Department of Forest Resources Hugo J. and Helen K. Pawek Fellowship; USDA Forest Service Northern Research Station FX We thank Meredith Cornett, Sawyer Scherer and Louise Potter, as well as the many people involved at the USDA Forest Service Northern Research Station in Grand Rapids, MN for their contributions to this work. Conversations with the Silviculture and Applied Forest Ecology Lab at The University of Minnesota (UMN) greatly improved this work. We thank two anonymous reviewers for comments on the manuscript. We thank UMN Department of Forest Resources Hugo J. and Helen K. Pawek Fellowship, as well as the USDA Forest Service Northern Research Station, for providing financial support for this project. We also want to thank the Chippewa National Forest for logistic support of the study. NR 58 TC 1 Z9 1 U1 22 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD AUG PY 2016 VL 53 IS 4 BP 1106 EP 1116 DI 10.1111/1365-2664.12656 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7FO UT WOS:000380065600015 ER PT J AU Hossain, MM Wilson, WC Faburay, B Richt, J McVey, DS Rowland, RR AF Hossain, Mohammad M. Wilson, William C. Faburay, Bonto Richt, Jurgen McVey, David S. Rowland, Raymond R. TI Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Fluorescence microsphere immunoassay; IgM antibody response; Recombinant RVFV proteins; Rift Valley fever virus ID DOMESTIC RUMINANTS; CLIMATE-CHANGE; VACCINATED ANIMALS; POTENTIAL VECTORS; INDIRECT ELISA; CAPTURE ELISA; UNITED-STATES; SAUDI-ARABIA; N-PROTEIN; NSM GENES AB A multiplex fluorescence microsphere immunoassay (FMIA) was used to detect bovine and ovine IgM and IgG antibodies to several Rift Valley fever virus (RVFV) proteins, including the major surface glycoprotein, Gn; the nonstructural proteins, NSs and NSm; and the nucleoprotein, N. Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural proteins, which can be applied to future development and validation of diagnostic tests that can be used to differentiate vaccinated from infected animals. C1 [Hossain, Mohammad M.; Faburay, Bonto; Richt, Jurgen; Rowland, Raymond R.] Kansas State Univ, Coll Vet Med, CEEZAD, Dept Diagnost Med Pathobiol, 1800 Denison Ave,N-211 Mosier Hall, Manhattan, KS 66506 USA. [Hossain, Mohammad M.; Wilson, William C.; McVey, David S.] ARS, USDA, ABADRU, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. RP Hossain, MM (reprint author), Kansas State Univ, Coll Vet Med, CEEZAD, Dept Diagnost Med Pathobiol, 1800 Denison Ave,N-211 Mosier Hall, Manhattan, KS 66506 USA. EM mofazzal@vet.k-state.edu FU Science and Technology Directorate of the U.S. Department of Homeland Security; Foreign Animal Disease Centers (Institute for Infectious Animal Diseases [IIAD] [HSHQDC-10-A-BOA33]; Center of Excellence for Emerging and Zoonotic Animal Diseases [CEEZAD] [2010-ST061-AG0001]; Kansas State NBAF Transition Funds; USDA Agricultural Research [5430-32000-005-00D] FX This work was supported by the Science and Technology Directorate of the U.S. Department of Homeland Security, Foreign Animal Disease Centers (Institute for Infectious Animal Diseases [IIAD] [HSHQDC-10-A-BOA33], Center of Excellence for Emerging and Zoonotic Animal Diseases [CEEZAD] [2010-ST061-AG0001]), Kansas State NBAF Transition Funds, and the USDA Agricultural Research project number 5430-32000-005-00D. The authors are grateful to Dr. H. Weingartl, Canadian Food Inspection Agency (CFIA), Dr. J. Morrill, University of Texas Medical Branch, and Dr. D. Watts, University of Texas-El Paso for providing serum samples from experimentally infected animals. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the USDA. NR 54 TC 1 Z9 1 U1 3 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 EI 1557-7759 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD AUG PY 2016 VL 16 IS 8 BP 550 EP 557 DI 10.1089/vbz.2014.1721 PG 8 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA DR7VR UT WOS:000380108300009 PM 27380552 ER PT J AU Allen, ST Edwards, BL Reba, ML Keim, RF AF Allen, Scott T. Edwards, Brandon L. Reba, Michele L. Keim, Richard F. TI Sub-canopy Evapotranspiration from Floating Vegetation and Open Water in a Swamp Forest SO WETLANDS LA English DT Article DE Water balance; Salvinia; Latent heat; Understory; Macrophytes; Wetland ID CRASSIPES MART SOLMS; PRAIRIE WETLAND; ENERGY-BUDGET; LEMNA-GIBBA; EVAPORATION; FLORIDA; EUTROPHICATION; TRANSPIRATION; MACROPHYTES; ECOSYSTEMS AB Among previous studies, there are large discrepancies in the difference between evapotranspiration from wetland vegetation and evaporation from open water. In this study, we investigate evapotranspiration differences between water and vegetation in a scenario that has otherwise not been extensively investigated: evapotranspiration from floodwaters in the sub-canopy environment. This study was conducted under a closed canopy baldcypress-ash-tupelo swamp forest in southeastern Louisiana. Water levels were measured in paired, partially-submerged evaporation pans, one with floating aquatic vegetation and the other without. Over the 5 month measurement period (June-November), average evapotranspiration rates from floating vegetation and open water were approximately 1.35 +/- 0.10 and 1.36 +/- 0.06 mm day(-1), respectively. Open water evaporation was generally higher in summer, and evapotranspiration from the vegetated water surface was higher in fall, likely due to changes in the sub-canopy energy environment related to both regional climate and site canopy phenology. C1 [Allen, Scott T.; Edwards, Brandon L.; Keim, Richard F.] Louisiana State Univ, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Reba, Michele L.] ARS, USDA, Natl Sedimentat Lab, Jonesboro, AR USA. RP Allen, ST (reprint author), Louisiana State Univ, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. EM salle48@lsu.edu FU Lucius W. Gilbert Foundation; Sigma Xi; Scientific Research Society; National Institute of Food and Agriculture, U.S. Department of Agriculture [LAB94181] FX Project funding was provided by the Lucius W. Gilbert Foundation, a Grant-In-Aid of Research from Sigma Xi, The Scientific Research Society, and the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award LAB94181. We thank the anonymous reviewers for their useful comments. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 43 TC 0 Z9 0 U1 4 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD AUG PY 2016 VL 36 IS 4 BP 681 EP 688 DI 10.1007/s13157-016-0778-z PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA DR7GC UT WOS:000380067000009 ER PT J AU Wierda, MR Leith, KF Roe, AS Grubb, TG Sikarskie, JG Best, DA Pittman, HT Fuentes, L Simon, KL Bowerman, W AF Wierda, Michael R. Leith, Katherine F. Roe, Amy S. Grubb, Teryl G. Sikarskie, James G. Best, David A. Pittman, H. Tyler Fuentes, Latice Simon, Kendall L. Bowerman, William TI Using bald eagles to track spatial (1999-2008) and temporal (1987-1992, 1999-2003, and 2004-2008) trends of contaminants in Michigan's aquatic ecosystems SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Pesticide; Wildlife toxicology; Aquatic toxicology; Biosentinel; Tertiary predator ID GREAT-LAKES BASIN; HALIAEETUS-ALBICILLA; ORGANOCHLORINE; EGGS; PRODUCTIVITY; REPRODUCTION; RESIDUES; BLOOD AB The bald eagle (Haliaeetus leucocephalus) is an extensively researched tertiary predator. Studies have delineated information about its life history and the influences of various stressors on its reproduction. Due to the bald eagle's position at the top of the food web, it is susceptible to biomagnification of xenobiotics. The Michigan Department of Environmental Quality implemented a program in 1999 to monitor persistent chemicals including polychlorinated biphenols (PCBs) and dichlorodiphenyltrichloroethane (DDE). The objectives of the present study were to evaluate spatial and temporal trends of PCBs and organochlorine pesticides in nestling bald eagles of Michigan. The authors' study found that concentrations of PCBs and DDE were higher in Great Lakes areas with Lakes Michigan and Lake Huron having the highest concentrations of DDE and Lake Erie having the highest concentrations of PCBs. Temporally (1987-1992, 1999-2003, and 2004-2008) the present study found declines in PCB and DDE concentrations with a few exceptions. Continued monitoring of Michigan bald eagle populations is suggested for a couple of reasons. First, nestling blood contaminant levels are an appropriate method to monitor ecosystem contaminant levels. Second, from 1999 to 2008 PCB and DDE concentrations for 30% and 40%, respectively, of the nestling eagles sampled were above the no observable adverse effect level (NOAEL) for bald eagles. Lastly, with the continued development and deployment of new chemistries a continuous long term monitoring program is an invaluable resource. Environ Toxicol Chem 2016;35:1995-2002. (c) 2016 SETAC C1 [Wierda, Michael R.; Leith, Katherine F.; Roe, Amy S.; Bowerman, William] Clemson Univ, Dept Forestry & Nat Resources, Clemson, SC 29634 USA. [Grubb, Teryl G.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ USA. [Sikarskie, James G.] Michigan State Univ, Vet Teaching Hosp, E Lansing, MI 48824 USA. [Best, David A.] US Fish & Wildlife Serv, Environm Contaminants Program, E Lansing, MI USA. [Pittman, H. Tyler; Fuentes, Latice; Simon, Kendall L.; Bowerman, William] Univ Maryland, Dept Environm Sci & Technol, College Pk, MD 20742 USA. RP Wierda, MR (reprint author), Clemson Univ, Dept Forestry & Nat Resources, Clemson, SC 29634 USA. EM mwierda@arizona.email.edu FU Michigan Department of Environmental Quality; Michigan Department of Natural Resources; US Forest Service; National Parks/Landholders; State Parks/Landholders; Private Parks/Landholders; Michigan State University; Clemson State University; Lake Superior State University FX Michigan Department of Environmental Quality, Michigan Department of Natural Resources, US Forest Service, National, State, and Private Parks/Landholders, Michigan, Clemson, and Lake Superior State Universities for their support. G. Hall, Research Programmer, Department of Astronomy, University of Arizona for visualization of data in the form of maps. Finally, the US Census Bureau and state of Michigan, DNR Fisheries Division or the University of Michigan, School of Natural Resources and Environment for map layers. NR 31 TC 0 Z9 0 U1 8 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD AUG PY 2016 VL 35 IS 8 BP 1995 EP 2002 DI 10.1002/etc.3523 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DR7CH UT WOS:000380057100014 PM 27442160 ER PT J AU Lindholm-Perry, AK Butler, AR Kern, RJ Hill, R Kuehn, LA Wells, JE Oliver, WT Hales, KE Foote, AP Freetly, HC AF Lindholm-Perry, A. K. Butler, A. R. Kern, R. J. Hill, R. Kuehn, L. A. Wells, J. E. Oliver, W. T. Hales, K. E. Foote, A. P. Freetly, H. C. TI Differential gene expression in the duodenum, jejunum and ileum among crossbred beef steers with divergent gain and feed intake phenotypes SO ANIMAL GENETICS LA English DT Article DE cattle; immune response; small intestine; stress response; transcriptome ID PREGNANT EWE LAMBS; DIETARY RESTRICTION; STRESS-RESPONSE; CATTLE; WEIGHT; CELLS; LIPOPOLYSACCHARIDE; GLUTATHIONE; EFFICIENCY; INTESTINE AB Small intestine mass and cellularity were previously associated with cattle feed efficiency. The small intestine is responsible for the digestion of nutrients and absorption of fatty acids, amino acids and carbohydrates, and it contributes to the overall feed efficiency of cattle. The objective of this study was to evaluate transcriptome differences among the small intestine from cattle with divergent gain and feed intake. Animals most divergent from the bivariate mean in each of the four phenotypic Cartesian quadrants for gainxintake were selected, and the transcriptomes of duodenum, jejunum and ileum were evaluated. Gene expression analyses were performed comparing high gain vs. low gain animals, high intake vs. low intake animals and each of the phenotypic quadrants to all other groups. Genes differentially expressed within the high gain-low intake and low gain-high intake groups of animals included those involved in immune function and inflammation in all small intestine sections. The high gain-high intake group differed from the high gain-low intake group by immune response genes in all sections of the small intestine. In all sections of small intestine, animals with low gain-low intake displayed greater abundance of heat-shock genes compared to other groups. Several over-represented pathways were identified. These include the antigen-processing/presentation pathway in high gain animals and PPAR signaling, starch/sucrose metabolism, retinol metabolism and melatonin degradation pathways in the high intake animals. Genes with functions in immune response, inflammation, stress response, influenza pathogenesis and melatonin degradation pathways may have a relationship with gain and intake in beef steers. C1 [Lindholm-Perry, A. K.; Kern, R. J.; Kuehn, L. A.; Wells, J. E.; Oliver, W. T.; Hales, K. E.; Foote, A. P.; Freetly, H. C.] ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. [Butler, A. R.] North Carolina State Univ, Raleigh, NC 27695 USA. [Hill, R.] Univ S Florida, Tampa, FL 33620 USA. RP Lindholm-Perry, AK (reprint author), ARS, USDA, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM amanda.lindholm@ars.usda.gov NR 37 TC 0 Z9 0 U1 4 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD AUG PY 2016 VL 47 IS 4 BP 408 EP 427 DI 10.1111/age.12440 PG 20 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA DR5IL UT WOS:000379936600002 PM 27226174 ER PT J AU Castillo, JM Grewell, BJ Pickart, AJ Figueroa, E Sytsma, M AF Castillo, Jesus M. Grewell, Brenda J. Pickart, Andrea J. Figueroa, Enrique Sytsma, Mark TI Variation in tussock architecture of the invasive cordgrass Spartina densiflora along the Pacific Coast of North America SO BIOLOGICAL INVASIONS LA English DT Article DE Anoxia; Climate change; Invasive species; Phenotypic plasticity; Salt marshes; Tussock traits ID SALT-MARSH; PHENOTYPIC PLASTICITY; CARBOHYDRATE ALLOCATION; EURASIAN WATERMILFOIL; LATITUDINAL GRADIENT; SEASONAL PATTERNS; PLANT ZONATION; GLOBAL CHANGE; EGERIA-DENSA; POPULATIONS AB Some introduced species spread rapidly beyond their native range and into novel habitats mediated by a high degree of phenotypic plasticity and/or rapid evolutionary responses. In this context, clonality has been described as a significant factor contributing to invasiveness. We studied the abiotic environment and the responses of different tussock architecture traits of the invasive cordgrass Spartina densiflora Brongn. (Poaceae). A common garden experiment and field studies of S. densiflora in salt marshes across a wide latitudinal gradient from California (USA) to British Columbia (Canada) provided a model system for an integrated study of the potential mechanisms underlying the response of invasive S. densiflora populations to changes in environmental conditions. Our results showed that S. densiflora is able to adjust to widely variable climate (specifically, air temperature and the duration of the growing season) and sediment conditions (specifically, texture and hypoxia) through phenotypical plastic key functional tussock traits (e.g. shoot density, height, above- and below-ground biomass allocation patterns). Root biomass increased in coarser sediments in contrast to rhizomes, which were more abundant in finer sediments. Above-ground biomass and leaf area index increased mainly with air temperature during summer, and more robust (taller and wider) shoots were associated with more oxygenated sediments. In view of our results, S. densiflora appears to be a halophyte with a high degree of phenotypic plasticity that would enable it to respond successfully to changes in the abiotic conditions of salt marshes driven by global climate change, such as increasing salinity and temperatures. C1 [Castillo, Jesus M.; Figueroa, Enrique] Univ Seville, Dept Biol Vegetal & Ecol, Seville, Spain. [Grewell, Brenda J.] Univ Calif Davis, USDA ARS Exot & Invas Weeds Res Unit, Davis, CA USA. [Pickart, Andrea J.] US Fish & Wildlife Serv, Humboldt Bay Natl Wildlife Refuge, Arcata, CA USA. [Sytsma, Mark] Portland State Univ, Ctr Lakes & Reservoirs, Portland, OR 97207 USA. RP Castillo, JM (reprint author), Univ Seville, Dept Biol Vegetal & Ecol, Seville, Spain. EM manucas@us.es FU Spanish Ministry of Education, Culture and Sport FX The authors thank D. Kerr, N. Mikkelsen and Ch. van Ossenbruggen for their assistance in the field. Thanks to M. Moscow, C. J. Futrell, J. Grant, R. Miller, S. Wells and C. Pena for their help in the laboratory. We are also grateful to the California Department of Food and Agriculture, Oregon Department of Agriculture, Washington State Department of Agriculture, Vancouver Island Conservation Land Management Program and Ducks Unlimited Canada for their assistance. Jesus M. Castillo thanks to the Spanish Ministry of Education, Culture and Sport for a grant of staff mobility for university teachers. five invasive populations of Spartina densiflora along the Pacific Coast of North America. Populations: SF San Francisco Bay Estuary, HB Humboldt Bay Estuary, MR Mad River Estuary, GH Grays Harbor Estuary, VI Vancouver Island (Baynes Sound) NR 78 TC 1 Z9 1 U1 13 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD AUG PY 2016 VL 18 IS 8 BP 2159 EP 2174 DI 10.1007/s10530-015-0991-3 PG 16 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7ZD UT WOS:000380117300005 ER PT J AU Grewell, BJ Castillo, JM Thomason, MJS Drenovsky, RE AF Grewell, Brenda J. Castillo, Jesus M. Thomason, Meghan J. Skaer Drenovsky, Rebecca E. TI Phenotypic plasticity and population differentiation in response to salinity in the invasive cordgrass Spartina densiflora SO BIOLOGICAL INVASIONS LA English DT Article DE Invasive species; Phenotypic plasticity; Plant invasions; Plant functional traits; Halophyte; Sea level rise ID SAN-FRANCISCO BAY; INVADING POPULATIONS; PLANT INVASIONS; LIFE-HISTORY; POLYPLOIDY; CALIFORNIA; STRESS; ANGLICA; MARSHES; GROWTH AB Salinity and tidal inundation induce physiological stress in vascular plant species and influence their distribution and productivity in estuarine wetlands. Climate change-induced sea level rise may magnify these abiotic stressors and the physiological stresses they can cause. Understanding the potential of invasive plants to respond to predicted salinity increases will elucidate their potential niche breadth. To examine potential phenotypic plasticity and functional trait responses to salinity stress in the invasive cordgrass Spartina densiflora, we collected rhizomes from four invasive populations occurring from California to Vancouver Island, British Columbia on the Pacific Coast of North America. In a glasshouse common garden experiment, we measured plant traits associated with growth and allocation, photosynthesis, leaf pigments, and leaf chemistry and calculated plasticity indices across imposed salinity treatments. Fifteen of 21 leaf chemistry, pigment, morphological and physiological traits expressed plastic responses to salinity. When averaged across all measured traits, degree of plasticity did not vary among sampled populations. However, differences in plasticity among populations in response to salinity were observed for 9 of 21 measured plant traits. Leaf chemistry and adaxial leaf rolling trait responses demonstrated the highest degree of plasticity, while growth and allocation measures were less plastic. Phenotypic plasticity of leaf functional traits to salinity indicates the potential of S. densiflora to maintain invasive growth in response to rising estuarine salinity with climate change. C1 [Grewell, Brenda J.; Thomason, Meghan J. Skaer] Univ Calif Davis, Dept Plant Sci, USDA ARS, Exot & Invas Weeds Res Unit, Davis, CA 95616 USA. [Castillo, Jesus M.] Univ Seville, Dept Biol Vegetal & Ecol, 1095 Apartado, Seville, Spain. [Drenovsky, Rebecca E.] John Carroll Univ, Dept Biol, University Hts, OH 44118 USA. RP Grewell, BJ (reprint author), Univ Calif Davis, Dept Plant Sci, USDA ARS, Exot & Invas Weeds Res Unit, Davis, CA 95616 USA. EM bjgrewell@ucdavis.edu OI Skaer Thomason, Meghan/0000-0001-9485-9729 NR 56 TC 0 Z9 0 U1 15 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD AUG PY 2016 VL 18 IS 8 BP 2175 EP 2187 DI 10.1007/s10530-015-1041-x PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR7ZD UT WOS:000380117300006 ER PT J AU Stromberg, JC Merritt, DM AF Stromberg, Juliet C. Merritt, David M. TI Riparian plant guilds of ephemeral, intermittent and perennial rivers SO FRESHWATER BIOLOGY LA English DT Article DE desert stream; plant functional type; riparian vegetation; trait; wood density ID SAN-PEDRO RIVER; FUNCTIONAL TRAITS; ENVIRONMENTAL FLOWS; COMMUNITY ECOLOGY; SPECIES TRAITS; SONORAN DESERT; UNITED-STATES; SURFACE-WATER; WOOD DENSITY; GLOBAL-SCALE AB Plant functional types (or guilds) increasingly are being used to predict vegetation response to global changes. Continued human population growth coupled with projected warmer and drier climate will alter the hydrologic regimes of many arid-zone rivers, including intermittent rivers. We aimed to identify (i) woody plant guilds associated with distinct stream types of an arid region and (ii) plant traits indicating adaptation to the selective pressures of water availability and fluvial disturbance. We used hierarchical clustering to identify 11 plant guilds from floodplains, terraces and uplands of eight Arizona rivers that vary in surface flow permanence, depth to ground water and intensity of fluvial disturbance. Six guilds were riparian pioneers with small, wind-dispersed seeds, three guilds were late-seral, shade-tolerant riparian taxa with large animal-dispersed seeds, and two guilds were composed of desert xerophytes. Within the riparian pioneer and seral groups, guilds varied in water acquisition and productivity traits including wood density and rooting depth. The community-weighted traits varied or covaried with water availability and fluvial disturbance. Root: shoot ratio, canopy height and leaf area were influenced strongly by water availability, with the latter two showing a nonlinear response to changes in water table depth. Leaf length increased, and wood density decreased, as sites become wetter and more fluvially disturbed. Community-weighted seed mass, seed dispersal and spinesence varied most strongly with elevation above thalweg (an indicator of decreasing fluvial disturbance). These analyses will enable prediction of changes in the relative abundance of plant types and plant traits in response to changes in stream flow regimes, such as shifts towards greater intermittency. The distribution patterns of guilds among riparian habitat types emphasise the importance of focusing conservation efforts not only on the limited number of perennial rivers remaining in arid regions, but also on intermittent and ephemeral rivers with shallow water tables. C1 [Stromberg, Juliet C.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Merritt, David M.] US Forest Serv, Watershed Fish Air & Rare Plants, Natl Stream & Aquat Ecol Ctr, Ft Collins, CO USA. RP Stromberg, JC (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. EM jstrom@asu.edu FU Environmental Protection Agency 'Science to Achieve Results' grant; Strategic Environmental Research and Development Program FX This study was funded by an Environmental Protection Agency 'Science to Achieve Results' grant and by the Strategic Environmental Research and Development Program. We thank Pima County Natural Resources Parks & Recreation Department, Pima Association of Governors and the Bureau of Land Management for providing data on stream hydrology or facilitating access to study sites; Danika Setaro, Jacqueline Betsch and Andrea F. Hazelton for sampling vegetation and identifying plants; Kristen Kaczynski for reviewing literature on wood density; and Thomas Kolb and Miles McCoy-Sulentic for sharing wood density data. Comments by Catherine Leigh, David Strayer and anonymous reviewers greatly improved the manuscript. NR 108 TC 1 Z9 1 U1 16 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0046-5070 EI 1365-2427 J9 FRESHWATER BIOL JI Freshw. Biol. PD AUG PY 2016 VL 61 IS 8 SI SI BP 1259 EP 1275 DI 10.1111/fwb.12686 PG 17 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA DR5PB UT WOS:000379953800007 ER PT J AU Ali, N Heslop-Harrison, JS Ahmad, H Graybosch, RA Hein, GL Schwarzacher, T AF Ali, N. Heslop-Harrison, J. S. (Pat) Ahmad, H. Graybosch, R. A. Hein, G. L. Schwarzacher, T. TI Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance SO HEREDITY LA English DT Article ID IN-SITU HYBRIDIZATION; RED WINTER-WHEAT; TRANSLOCATION LINES; THINOPYRUM-PONTICUM; MOLECULAR MARKERS; GENE; RYE; IDENTIFICATION; REGISTRATION; INTERMEDIUM AB Pyramiding of alien-derived Wheat streak mosaic virus (WSMV) resistance and resistance enhancing genes in wheat is a cost-effective and environmentally safe strategy for disease control. PCR-based markers and cytogenetic analysis with genomic in situ hybridisation were applied to identify alien chromatin in four genetically diverse populations of wheat (Triticum aestivum) lines incorporating chromosome segments from Thinopyrum intermedium and Secale cereale (rye). Out of 20 experimental lines, 10 carried Th. intermedium chromatin as T4DL*4Ai#2S translocations, while, unexpectedly, 7 lines were positive for alien chromatin (Th. intermedium or rye) on chromosome 1B. The newly described rye 1RS chromatin, transmitted from early in the pedigree, was associated with enhanced WSMV resistance. Under field conditions, the 1RS chromatin alone showed some resistance, while together with the Th. intermedium 4Ai#2S offered superior resistance to that demonstrated by the known resistant cultivar Mace. Most alien wheat lines carry whole chromosome arms, and it is notable that these lines showed intra-arm recombination within the 1BS arm. The translocation breakpoints between 1BS and alien chromatin fell in three categories: (i) at or near to the centromere, (ii) intercalary between markers UL-Thin5 and Xgwm1130 and (iii) towards the telomere between Xgwm0911 and Xbarc194. Labelled genomic Th. intermedium DNA hybridised to the rye 1RS chromatin under high stringency conditions, indicating the presence of shared tandem repeats among the cereals. The novel small alien fragments may explain the difficulty in developing well-adapted lines carrying Wsm1 despite improved tolerance to the virus. The results will facilitate directed chromosome engineering producing agronomically desirable WSMV-resistant germplasm. C1 [Ali, N.; Heslop-Harrison, J. S. (Pat); Schwarzacher, T.] Univ Leicester, Dept Genet, Univ Rd, Leicester LE1 7RH, Leics, England. [Ali, N.; Ahmad, H.] Hazara Univ, Mansehra, Pakistan. [Graybosch, R. A.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [Graybosch, R. A.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Hein, G. L.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. RP Schwarzacher, T (reprint author), Univ Leicester, Dept Genet, Univ Rd, Leicester LE1 7RH, Leics, England. EM ts32@le.ac.uk RI Ahmad, Habib/L-9669-2016; OI Heslop-Harrison, J.S. (Pat)/0000-0002-3105-2167 FU Hazara University, Mansehra; Higher Education Commission (HEC), Pakistan; United States Wheat Board FX We thank Hazara University, Mansehra and Higher Education Commission (HEC), Pakistan for PhD fellowship to NA. RAG and GLH acknowledge the United States Wheat Board for support. We thank Steve Reader, John Innes Centre, Norwich, UK, for providing nullisomic-tetrasomic wheat lines, Marion S. Roder (IPK, Gatersleben Germany) for providing sequences of unpublished oligonucleotides. NR 42 TC 2 Z9 2 U1 9 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD AUG PY 2016 VL 117 IS 2 BP 114 EP 123 DI 10.1038/hdy.2016.36 PG 10 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA DR7CW UT WOS:000380058600007 PM 27245423 ER PT J AU Alandia, G Jacobsen, SE Kyvsgaard, NC Condori, B Liu, F AF Alandia, G. Jacobsen, S. -E. Kyvsgaard, N. C. Condori, B. Liu, F. TI Nitrogen Sustains Seed Yield of Quinoa Under Intermediate Drought SO JOURNAL OF AGRONOMY AND CROP SCIENCE LA English DT Article DE abiotic stress; drylands; fertilization; tolerance ID WATER-USE EFFICIENCY; LEAF GAS-EXCHANGE; CHENOPODIUM-QUINOA; WINTER-WHEAT; DEFICIT IRRIGATION; NUTRITION; PLANTS; WILLD.; RESPONSES; STRESS AB Quinoa (Chenopodium quinoa Willd.) is a promising crop for food security in dry areas. Studies have been conducted to define nitrogen (N) fertilization levels and to understand the responses of quinoa to drought, but little is known about the response of this crop to N fertilization under drought stress. The aim of this study was to investigate whether N fertilization could improve quinoa yield and physiology under limited water. A greenhouse experiment was carried out with quinoa grown at four N fertilization levels (0, 0.2, 0.4 and 0.6g N pot(-1)) and two watering treatments (progressive drought and full irrigation; 10 and 98% of pot water holding capacity, respectively). Results of this experiment showed that N may confer a certain degree of drought tolerance to quinoa as seed quality and yield of N-fertilized plants were not affected by drought stress. Responses such as faster stomatal closure, reduced leaf water potential, higher leaf abscisic acid (ABA) concentration and particularly an improved N remobilization in N-fertilized plants may have played a role in sustaining seed yield in the drought-stressed treatment. These results under controlled conditions serve as a basis to elucidate drought tolerance mechanisms activated with N fertilization and to define the use of N in management practices under semi-arid environments. C1 [Alandia, G.; Jacobsen, S. -E.; Liu, F.] Univ Copenhagen, Dept Plant & Environm Sci, Fac Sci, Hojbakkegaard 13, DK-2630 Taastrup, Denmark. [Alandia, G.] PROINPA Fdn, El Paso Expt Ctr, Cochabamba, Bolivia. [Kyvsgaard, N. C.] Danish Hlth & Med Author, Copenhagen, Denmark. [Condori, B.] ARS, USDA, Crop Syst & Global Change, Beltsville, MD USA. RP Alandia, G (reprint author), Univ Copenhagen, Dept Plant & Environm Sci, Fac Sci, Hojbakkegaard 13, DK-2630 Taastrup, Denmark. EM gar@plen.ku.dk RI Liu, Fulai/D-8357-2013; Condori, Bruno/E-7644-2013; OI Liu, Fulai/0000-0002-5006-8965; Condori, Bruno/0000-0001-6537-0625; Jacobsen, Sven-Erik/0000-0002-9596-929X FU Danish cooperation DANIDA through the ANDESCROP project [09-058LIFE]; University of Copenhagen FX This study was funded by the Danish cooperation DANIDA through the ANDESCROP project (09-058LIFE) with cooperation received from the University of Copenhagen. The authors wish to thank the help of Yujie Sun and the technical staff at the University of Copenhagen as well as the guidance with 'R' software received from Jens Carl Streibig. The valuable help provided by Jens Erik Orum during fertilization and Muhammad Amjad during the experiment harvesting time is gratefully acknowledged. Dominik Kilian Grosskinsky is also thanked for his helpful comments on the manuscript. NR 43 TC 1 Z9 1 U1 10 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2250 EI 1439-037X J9 J AGRON CROP SCI JI J. Agron. Crop Sci. PD AUG PY 2016 VL 202 IS 4 BP 281 EP 291 DI 10.1111/jac.12155 PG 11 WC Agronomy SC Agriculture GA DR6OM UT WOS:000380021200003 ER PT J AU Karsch, RC Cain, JW Rominger, EM Goldstein, EJ AF Karsch, Rebekah C. Cain, James W., III Rominger, Eric M. Goldstein, Elise J. TI Desert bighorn sheep lambing habitat: Parturition, nursery, and predation sites SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE birth site; desert bighorn sheep; lamb survival; Ovis canadensis mexicana; parturition; predation; vaginal-implant transmitter ID WHITE-TAILED DEER; MOUNTAIN SHEEP; SEXUAL SEGREGATION; ANTIPREDATOR TACTICS; POPULATION-DYNAMICS; LARGE HERBIVORES; SELECTION; BIRTH; SURVIVAL; MORTALITY AB Fitness of female ungulates is determined by neonate survival and lifetime reproductive success. Therefore, adult female ungulates should adopt behaviors and habitat selection patterns that enhance survival of neonates during parturition and lactation. Parturition site location may play an important role in neonatal mortality of desert bighorn sheep (Ovis canadensis mexicana) when lambs are especially vulnerable to predation, but parturition sites are rarely documented for this species. Our objectives were to assess environmental characteristics at desert bighorn parturition, lamb nursery, and predation sites and to assess differences in habitat characteristics between parturition sites and nursery group sites, and predation sites and nursery group sites. We used vaginal implant transmitters (VITs) to identify parturition sites and capture neonates. We then compared elevation, slope, terrain ruggedness, and visibility at parturition, nursery, and lamb predation sites with paired random sites and compared characteristics of parturition sites and lamb predation sites to those of nursery sites. When compared to random sites, odds of a site being a parturition site were highest at intermediate slopes and decreased with increasing female visibility. Odds of a site being a predation site increased with decreasing visibility. When compared to nursery group sites, odds of a site being a parturition site had a quadratic relationship with elevation and slope, with odds being highest at intermediate elevations and intermediate slopes. When we compared predation sites to nursery sites, odds of a site being a predation were highest at low elevation areas with high visibility and high elevation areas with low visibility likely because of differences in hunting strategies of coyote (Canis latrans) and puma (Puma concolor). Parturition sites were lower in elevation and slope than nursery sites. Understanding selection of parturition sites by adult females and how habitat characteristics at these sites differ from those at predation and nursery sites can provide insight into strategies employed by female desert bighorn sheep and other species during and after parturition to promote neonate survival. (c) 2016 The Wildlife Society. C1 [Karsch, Rebekah C.] New Mexico State Univ, Dept Fish Wildlife & Conservat Ecol, POB 30003,MSC 4901, Las Cruces, NM 88003 USA. [Cain, James W., III] New Mexico State Univ, US Geol Survey, New Mexico Cooperat Fish & Wildlife Res Unit, Dept Fish Wildlife & Conservat Ecol, POB 30003,MSC 4901, Las Cruces, NM 88033 USA. [Rominger, Eric M.; Goldstein, Elise J.] New Mexico Dept Game & Fish, 1 Wildlife Way, Santa Fe, NM 87507 USA. [Karsch, Rebekah C.] US Forest Serv, Coronado Natl Forest, 300 West Congress St, Tucson, AZ 85701 USA. RP Cain, JW (reprint author), New Mexico State Univ, US Geol Survey, New Mexico Cooperat Fish & Wildlife Res Unit, Dept Fish Wildlife & Conservat Ecol, POB 30003,MSC 4901, Las Cruces, NM 88033 USA. EM jwcain@nmsu.edu FU New Mexico Department of Game and Fish; New Mexico Chapter of the Wild Sheep Foundation; Desert Bighorn Council; Houston Safari Club; T E FX Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. We thank J. Ross for her assistance determining pregnancy in female bighorn and inserting VITs and T. T. Turnbull, E. Murray, J. B. Ashling, K. R. Garrison, M. J. Gould, B. Long, M. Overstreet, and J. W. Pitman for assistance with lamb captures and field work. Comments by M. Festa-Bianchet, J. V. Gedir, P. R. Krausman, and an anonymous reviewer improved an earlier draft of this manuscript. New Mexico Department of Game and Fish, New Mexico Chapter of the Wild Sheep Foundation, Desert Bighorn Council, the Houston Safari Club, and T & E provided funding and support. NR 77 TC 0 Z9 0 U1 17 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2016 VL 80 IS 6 BP 1069 EP 1080 DI 10.1002/jwmg.21092 PG 12 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DR5RI UT WOS:000379959700012 ER PT J AU Kierepka, EM Unger, SD Keiter, DA Beasley, JC Rhodes, OE Cunningham, FL Piaggio, AJ AF Kierepka, Elizabeth M. Unger, Shem D. Keiter, David A. Beasley, James C. Rhodes, Olin E., Jr. Cunningham, Fred L. Piaggio, Antoinette J. TI Identification of robust microsatellite markers for wild pig fecal DNA SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE degradation; feces; microsatellites; noninvasive; South Carolina; Sus scrofa; wild pigs ID GENOTYPING ERROR RATES; MIXED-EFFECTS MODELS; POPULATION-SIZE; URSUS-ARCTOS; FERAL SWINE; SUS-SCROFA; NONINVASIVE SAMPLES; CANIS-RUFUS; AMPLIFICATION; COLLECTION AB Collection of fecal samples for use in a genetic capture-mark-recapture framework has become popular as a noninvasive method of monitoring wildlife populations. A major caveat to this process, however, is that fecal samples often yield low quality DNA that is prone to genotyping errors, potentially leading to biases in population parameter estimation. Therefore, considerable care is required to identify robust genetic markers, especially in hot or humid conditions that may accelerate DNA degradation. We identified microsatellite loci in wild pig (Sus scrofa) fecal samples that were robust and informative within warm, humid ecosystems. To examine how degradation affected genotyping success, we sampled pig feces across 5 days and calculated how the number of quantitative polymerase chain reaction (qPCR) cycles required to reach the fluorescent threshold (C-t) changed over time. We identified 17 microsatellite loci that had high polymorphism and amplification success and low genotyping error rates (0-0.050 per locus). In the degradation experiment, C-t increased over the 5 days, but in the absence of rain, the majority of samples produced accurate genotypes after 5 days (2,211/2,550 genotypes). Based on the high amplification success and low error rates, even after 5 days of exposure to warm, humid conditions, these loci are useful for estimating population parameters in pig fecal samples. (c) 2016 The Wildlife Society. C1 [Kierepka, Elizabeth M.; Unger, Shem D.; Rhodes, Olin E., Jr.] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA. [Keiter, David A.; Beasley, James C.] Univ Georgia, Savannah River Ecol Lab, Warnell Sch Forestry & Nat Resources, Aiken, SC 29802 USA. [Cunningham, Fred L.] Wildlife Serv, USDA, Mississippi Field Stn, Natl Wildlife Res Ctr, POB 6099, Mississippi State, MS 39762 USA. [Piaggio, Antoinette J.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. RP Kierepka, EM (reprint author), Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA. EM liz.kierepka@gmail.com FU University of Georgia Research Foundation; U.S. Department of Agriculture's National Wildlife Research Center [14-7408-1046-CA]; U.S. Department of Energy [DE-FC09-07SR22506] FX We thank the private contractors that culled pigs for this study and field technicians E. K. Bledsoe, K. D. Eckert, and R. L. Juarez for collecting fecal and tissue samples. This work was supported by Cooperative Agreements between the University of Georgia Research Foundation and both the U.S. Department of Agriculture's National Wildlife Research Center (no. 14-7408-1046-CA) and the U.S. Department of Energy (DE-FC09-07SR22506). NR 58 TC 0 Z9 0 U1 13 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2016 VL 80 IS 6 BP 1120 EP 1128 DI 10.1002/jwmg.21102 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA DR5RI UT WOS:000379959700016 ER PT J AU McGarigal, K Zeller, KA Cushman, SA AF McGarigal, Kevin Zeller, Kathy A. Cushman, Samuel A. TI Multi-scale habitat selection modeling: introduction to the special issue SO LANDSCAPE ECOLOGY LA English DT Editorial Material ID ECOLOGY; SCALE C1 [McGarigal, Kevin; Zeller, Kathy A.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP McGarigal, K (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. EM mcgarigalk@eco.umass.edu NR 13 TC 0 Z9 0 U1 18 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1157 EP 1160 DI 10.1007/s10980-016-0388-4 PG 4 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300001 ER PT J AU McGarigal, K Wan, HY Zeller, KA Timm, BC Cushman, SA AF McGarigal, Kevin Wan, Ho Yi Zeller, Kathy A. Timm, Brad C. Cushman, Samuel A. TI Multi-scale habitat selection modeling: a review and outlook SO LANDSCAPE ECOLOGY LA English DT Article DE Scale; Habitat; Habitat selection; Resource selection; Resource selection function; Wildlife habitat relationships; Species-environment relationships ID MULTIPLE SPATIAL SCALES; RESOURCE SELECTION; ANIMAL MOVEMENT; ECOLOGY; COMMUNITY; PATTERNS; CARIBOU; BIRDS AB Scale is the lens that focuses ecological relationships. Organisms select habitat at multiple hierarchical levels and at different spatial and/or temporal scales within each level. Failure to properly address scale dependence can result in incorrect inferences in multi-scale habitat selection modeling studies. Our goals in this review are to describe the conceptual origins of multi-scale habitat selection modeling, evaluate the current state-of-the-science, and suggest ways forward to improve analysis of scale-dependent habitat selection. We reviewed more than 800 papers on habitat selection from 23 major ecological journals published between 2009 and 2014 and recorded a number of characteristics, such as whether they addressed habitat selection at multiple scales, what attributes of scale were evaluated, and what analytical methods were utilized. Our results show that despite widespread recognition of the importance of multi-scale analyses of habitat relationships, a large majority of published habitat ecology papers do not address multiple spatial or temporal scales. We also found that scale optimization, which is critical to assess scale dependence, is done in less than 5 % of all habitat selection modeling papers and less than 25 % of papers that address "multi-scale" habitat analysis broadly defined. Our review confirms the existence of a powerful conceptual foundation for multi-scale habitat selection modeling, but that the majority of studies on wildlife habitat are still not adopting multi-scale frameworks. Most importantly, our review points to the need for wider adoption of a formal scale optimization of organism response to environmental variables. C1 [McGarigal, Kevin; Zeller, Kathy A.; Timm, Brad C.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Wan, Ho Yi] No Arizona Univ, Sch Earth Sci & Environm Sustainabil, Flagstaff, AZ 86001 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP McGarigal, K (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. EM mcgarigalk@eco.umass.edu RI Wan, Ho Yi/E-8858-2011 OI Wan, Ho Yi/0000-0002-2146-8257 FU National Science Foundation [DGE-0907995]; Joint Fire Sciences Project [12-1-06-56] FX This work was supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-0907995 (Zeller) and the Joint Fire Sciences Project # 12-1-06-56 (Wan, Timm, McGarigal, and Cushman). This manuscript was also improved by the suggestions of several anonymous reviewers. NR 47 TC 5 Z9 5 U1 43 U2 65 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1161 EP 1175 DI 10.1007/s10980-016-0374-x PG 15 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300002 ER PT J AU Timm, BC McGarigal, K Cushman, SA Ganey, JL AF Timm, Brad C. McGarigal, Kevin Cushman, Samuel A. Ganey, Joseph L. TI Multi-scale Mexican spotted owl (Strix occidentalis lucida) nest/roost habitat selection in Arizona and a comparison with single-scale modeling results SO LANDSCAPE ECOLOGY LA English DT Article DE Multi-scale modeling; Habitat; Scale; All-subsets; Scaling; Spotted owl; Habitat; Selection; Habitat preference ID CENTRAL SIERRA-NEVADA; ROOST SITES; TULAROSA MOUNTAINS; PACIFIC-NORTHWEST; NESTING HABITAT; LANDSCAPE; CONSERVATION; RESTORATION; FORESTS; ASSOCIATIONS AB Organisms commonly respond to their environment across a range of scales, however many habitat selection studies still conduct selection analyses using a single-scale framework. The adoption of multi-scale modeling frameworks in habitat selection studies can improve the effectiveness of these studies and provide greater insights into scale-dependent relationships between species and specific habitat components. Our study assessed multi-scale nest/roost habitat selection of the federally "Threatened" Mexican spotted owl (Strix occidentalis lucida) in northern Arizona, USA in an effort to provide improved conservation and management strategies for this subspecies. We conducted multi-scale habitat modeling to assess habitat selection by Mexican spotted owls using survey data collected by the USFS. Each selected covariate was included in multi-scale models at their "characteristic scale" and we used an all-subsets approach and model selection framework to assess habitat selection. The "characteristic scale" identified for each covariate varied considerably among covariates and results from multi-scale models indicated that percent canopy cover and slope were the most important covariates with respect to habitat selection by Mexican spotted owls. Multi-scale models consistently outperformed their analogous single-scale counterparts with respect to the proportion of deviance explained and model predictive performance. Efficacy of future habitat selection studies will benefit by taking a multi-scale approach. In addition to potentially providing increased explanatory power and predictive capacity, multi-scale habitat models enhance our understanding of the scales at which species respond to their environment, which is critical knowledge required to implement effective conservation and management strategies. C1 [Timm, Brad C.; McGarigal, Kevin] Univ Massachusetts, Dept Environm Conservat, Holdsworth Nat Resources Ctr, Amherst, MA 01003 USA. [Cushman, Samuel A.; Ganey, Joseph L.] US Forest Serv, Rocky Mt Res Stn, USDA, 2500 South Pine Knoll Dr, Flagstaff, AZ 86001 USA. RP Timm, BC (reprint author), Univ Massachusetts, Dept Environm Conservat, Holdsworth Nat Resources Ctr, Amherst, MA 01003 USA. EM bradtimm@gmail.com FU Joint Fire Science Program [12-1-06-56] FX We thank S. Hedwall, J. Jenness, and S. Sesnie for valuable input throughout various stages of this manuscript preparation. This research was funded primarily by the Joint Fire Science Program (Project ID: 12-1-06-56). This is manuscript XXXXX of the University of Massachusetts-Amherst Agricultural Extension. NR 60 TC 0 Z9 0 U1 20 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1209 EP 1225 DI 10.1007/s10980-016-0371-0 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300005 ER PT J AU Vergara, M Cushman, SA Urra, F Ruiz-Gonzalez, A AF Vergara, Maria Cushman, Samuel A. Urra, Fermin Ruiz-Gonzalez, Aritz TI Shaken but not stirred: multiscale habitat suitability modeling of sympatric marten species (Martes martes and Martes foina) in the northern Iberian Peninsula SO LANDSCAPE ECOLOGY LA English DT Article DE HSM; Scale dependency; Sampling bias; Niche divergence; Maxent; Pine marten; Stone marten ID STONE MARTENS; SAMPLING BIAS; SPATIAL SCALE; CENTRAL SPAIN; DISTRIBUTIONS; MAXENT; SELECTION; PATTERNS; FOREST; CARNIVORES AB Multispecies and multiscale habitat suitability models (HSM) are important to identify the environmental variables and scales influencing habitat selection and facilitate the comparison of closely related species with different ecological requirements. This study explores the multiscale relationships of habitat suitability for the pine (Martes martes) and stone marten (M. foina) in northern Spain to evaluate differences in habitat selection and scaling, and to determine if there is habitat niche displacement when both species coexist. We combined bivariate scaling and maximum entropy modeling to compare the multiscale habitat selection of the two martens. To optimize the HSM, the performance of three sampling bias correction methods at four spatial scales was explored. HSMs were compared to explore niche differentiation between species through a niche identity test. The comparison among HSMs resulted in the detection of a significant niche divergence between species. The pine marten was positively associated with cooler mountainous areas, low levels of human disturbance, high proportion of natural forests and well-connected forestry plantations, and medium-extent agroforestry mosaics. The stone marten was positively related to the density of urban areas, the proportion and extensiveness of croplands, the existence of some scrub cover and semi-continuous grasslands. This study outlines the influence of the spatial scale and the importance of the sampling bias corrections in HSM, and to our knowledge, it is the first comparing multiscale habitat selection and niche divergence of two related marten species. This study provides a useful methodological framework for multispecies and multiscale comparatives. C1 [Vergara, Maria; Ruiz-Gonzalez, Aritz] Univ Basque Country, Dept Zool & Anim Cell Biol, UPV EHU, C Paseo Univ 7, Vitoria 01006, Spain. [Vergara, Maria; Ruiz-Gonzalez, Aritz] Univ Basque Country, Systemat Biogeog & Populat Dynam Res Grp, UPV EHU, Lascaray Res Ctr, Avda Miguel Unamuno 3, Vitoria 01006, Spain. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Urra, Fermin] Environm Management Navarre SA, Biodivers Unit, C Padre Adoain 219 Bajo, Pamplona 31015, Spain. RP Vergara, M (reprint author), Univ Basque Country, Dept Zool & Anim Cell Biol, UPV EHU, C Paseo Univ 7, Vitoria 01006, Spain. EM m1v2r3@hotmail.com OI RUIZ GONZALEZ, ARITZ/0000-0001-7409-4634 FU Basque Government through the Research group on "Systematics, Biogeography and Population Dynamics" [IT317-10, GIC10/76]; Department of Education, Universities and Research of the Basque Government [RBFI-2012-446, DKR-2012-64]; U.S. Forest Service Rocky Mountain Research Station; Habitat section, Department of rural development, environment and local administrations, Navarre Government FX This study has been partially funded by the Basque Government through the Research group on "Systematics, Biogeography and Population Dynamics" (Ref. IT317-10; GIC10/76). MV (Ref: RBFI-2012-446) and ARG (Ref: DKR-2012-64) were supported by a PhD and post-doctoral fellowships awarded by the Department of Education, Universities and Research of the Basque Government. U.S. Forest Service Rocky Mountain Research Station supported Cushman's work on this project. The authors wish to thank all the people directly involved in the collection of non-invasive genetic samples and those reporting the tissue specimens and species locations used in this study, including rangers, vets and field researchers and their institutions (Table 51 in Online Appendix). The sampling of Navarre region was supported by the Habitat section, Department of rural development, environment and local administrations, Navarre Government. We are also very grateful to Dr. Jason Brown and Dr. Warren for their useful comments regarding SDMtools and ENMtools, respectively. NR 74 TC 2 Z9 2 U1 13 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1241 EP 1260 DI 10.1007/s10980-015-0307-0 PG 20 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300007 ER PT J AU Rostro-Garcia, S Tharchen, L Abade, L Astaras, C Cushman, SA Macdonald, DW AF Rostro-Garcia, Susana Tharchen, Lhendup Abade, Leandro Astaras, Christos Cushman, Samuel A. Macdonald, David W. TI Scale dependence of felid predation risk: identifying predictors of livestock kills by tiger and leopard in Bhutan SO LANDSCAPE ECOLOGY LA English DT Article DE Carnivore conservation; Human-carnivore conflict; Livestock predation; MaxEnt; Multi-scale analysis; Panthera pardus; Panthera tigris; Spatial predation risk modeling; Scale optimization ID HUMAN-WILDLIFE CONFLICT; HUMAN-CARNIVORE CONFLICT; WANGCHUCK NATIONAL-PARK; AMUR SIBERIAN TIGERS; JAGUAR PANTHERA-ONCA; SPECIES DISTRIBUTIONS; HABITAT SELECTION; PROTECTED AREAS; PUMA-CONCOLOR; GENE FLOW AB Livestock predation by tiger and leopard in Bhutan is a major threat to the conservation of these felids. Conflict mitigation planning would benefit from an improved understanding of the spatial pattern of livestock kills by the two predators. We aimed to identify the landscape features that predict livestock kills by tiger and leopard throughout Bhutan. Our goals were to: (1) identify the predictors that have the largest influence in determining livestock kills, (2) assess the influence of scale across the different predictors evaluated and identify the scale at which each was most important. We used livestock kills obtained from compensation records of tiger (n = 326) and leopard (n = 377) across Bhutan between 2003 and 2012 to run predation risk models with MaxEnt algorithm, using a multi-scale modeling approach (1, 2, 4, 8 and 16 km). Human-presence (density of settlements and roads) and land-cover (percentage of tree cover and meadow patches) were the main variables contributing to livestock kills by both species. Livestock kills were likely driven by a trade-off between livestock density and predator ecology, and the balance of this trade-off varied with scale. Risk maps revealed different hotspots for tiger and leopard kills, and analysis showed both species preferentially killed equids over other livestock types. Our results highlight the importance of evaluating scale when investigating the spatial attributes of livestock kills by tiger and leopard. Our findings provide guidance for reducing conflict between humans and large felids throughout the country. C1 [Rostro-Garcia, Susana; Abade, Leandro; Astaras, Christos; Macdonald, David W.] Univ Oxford, Dept Zool, Wildlife Conservat Res Unit, Recanati Kaplan Ctr, Tubney House,Abingdon Rd, Tubney OX13 5QL, England. [Tharchen, Lhendup] Jigme Dorji Natl Pk, Dept Forest & Pk Serv, Gasa, Bhutan. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Rostro-Garcia, S (reprint author), Univ Oxford, Dept Zool, Wildlife Conservat Res Unit, Recanati Kaplan Ctr, Tubney House,Abingdon Rd, Tubney OX13 5QL, England. EM rostro.susana@gmail.com FU Rufford Foundation (Rufford Small Grant funding scheme) FX We thank the Department of Forest and Park Services, Royal Government of Bhutan for making the livestock predation records available to us for the purposes of this study. We are also grateful to the park and forest officials, veterinarians and community leaders involved in gathering the data. Thanks to Arjun Gopalaswamy, Jan F. Kamler and anonymous reviewers for providing feedback. Funding for the digitizing of the records and disseminations of the preliminary findings of the study was provided by the Rufford Foundation (Rufford Small Grant funding scheme). This work was done primarily while SRG was at WildCRU undertaking the International Postgraduate Diploma in Practical Wildlife Conservation Practice at the Recanati-Kaplan Centre. NR 105 TC 0 Z9 0 U1 48 U2 57 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1277 EP 1298 DI 10.1007/s10980-015-0335-9 PG 22 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300009 ER PT J AU Chambers, CL Cushman, SA Medina-Fitoria, A Martinez-Fonseca, J Chavez-Velasquez, M AF Chambers, Carol L. Cushman, Samuel A. Medina-Fitoria, Arnulfo Martinez-Fonseca, Jose Chavez-Velasquez, Marlon TI Influences of scale on bat habitat relationships in a forested landscape in Nicaragua SO LANDSCAPE ECOLOGY LA English DT Article DE Scale-dependent habitat selection; Landscape metrics; Landscape composition; Landscape configuration; Chiroptera; Forest fragmentation; FRAGSTATS; Multi-scale habitat modeling ID TROPICAL DRY FORESTS; HOME-RANGE SIZE; PHYLLOSTOMID BATS; CENTRAL-AMERICA; AGRICULTURAL LANDSCAPE; SPECIES RICHNESS; NORTHERN ARIZONA; DIVERSITY; FRAGMENTATION; SELECTION AB Scale dependence of bat habitat selection is poorly known with few studies evaluating relationships among landscape metrics such as class versus landscape, or metrics that measure composition or configuration. This knowledge can inform conservation approaches to mitigate habitat loss and fragmentation. We evaluated scale dependence of habitat associations and scaling patterns of landscape metrics in relation to bat occurrence or capture rate in forests of southwestern Nicaragua. We captured 1537 bats at 35 locations and measured landscape and class metrics across 10 spatial scales (100-1000 m) surrounding capture locations. We conducted univariate scaling across the 10 scales and identified scales and variables most related to bat occurrence or capture rate. Edge and patch density, at both landscape and class levels, were the most important variables across species. Feeding guilds varied in their response to metrics. Certain landscape and configuration metrics were most influential at fine (100 m) and/or broad (1000 m) spatial scales while most class and composition metrics were influential at intermediate scales. These results provide insight into the scale dependence of habitat associations of bat species and the influence of fine and broad scales on habitat associations. The effects of scale, examined in our study and others from fine (100 m) to broad (5 km) indicate habitat relationships for bats may be more informative at larger scales. Our results suggest there could be general differences in scale relationships for different groups of landscape metrics, which deserves further evaluation in other taxonomic groups. C1 [Chambers, Carol L.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Medina-Fitoria, Arnulfo; Martinez-Fonseca, Jose; Chavez-Velasquez, Marlon] Managua Nicaragua & Paso Pacifico, Program Conservat Bats Nicaragua PCMN, Carretera Masaya Km 12-4,Casa 7, Managua, Nicaragua. RP Chambers, CL (reprint author), No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. EM carol.chambers@nau.edu FU Conservation International; Percy Sladen Memorial Fund FX We thank the Bat Conservation International and the Percy Sladen Memorial Fund for financial support. Paso Pacffio provided logistic and field support. We thank landowners who provided access to their property for this work including Miguel Melendez and Miguel Soto. We thank J. Crouse for the study area map. Volunteers who helped capture, identify, and radio track bats included D. Brown, B. Burger, C. Corben, K. Day, A. Haskew, K. Livengood, A. McIntire, B. Noble, L. Piest, E. Rutherford, M. Siders, D. Sinton, T. Snow, B. Taubert, D. Taylor, S. Tuttle, and K. Williams-Guillen. The Associate Editor and 2 anonymous reviewers provided comments that greatly improved the manuscript. NR 75 TC 1 Z9 1 U1 6 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1299 EP 1318 DI 10.1007/s10980-016-0343-4 PG 20 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300010 ER PT J AU Zeller, KA McGarigal, K Cushman, SA Beier, P Vickers, TW Boyce, WM AF Zeller, Katherine A. McGarigal, Kevin Cushman, Samuel A. Beier, Paul Vickers, T. Winston Boyce, Walter M. TI Using step and path selection functions for estimating resistance to movement: pumas as a case study SO LANDSCAPE ECOLOGY LA English DT Article DE Puma concolor; Resistance surface; Connectivity; Corridors; Wildlife; Multi-scale habitat modeling ID LANDSCAPE CONNECTIVITY; RESOURCE SELECTION; SOUTHERN CALIFORNIA; HABITAT-SELECTION; BEHAVIOR; SCALE; CONSERVATION; PERFORMANCE; DISTANCE; ROADS AB GPS telemetry collars and their ability to acquire accurate and consistently frequent locations have increased the use of step selection functions (SSFs) and path selection functions (PathSFs) for studying animal movement and estimating resistance. However, previously published SSFs and PathSFs often do not accommodate multiple scales or multi-scale modeling. We present a method that allows multiple scales to be analyzed with SSF and PathSF models. We also explore the sensitivity of model results and resistance surfaces to whether SSFs or PathSFs are used, scale, prediction framework, and GPS collar sampling interval. We use 5-min GPS collar data from pumas (Puma concolor) in southern California to model SSFs and PathSFs at multiple scales, to predict resistance using two prediction frameworks (paired and unpaired), and to explore potential bias from GPS collar sampling intervals. Regression coefficients were extremely sensitive to scale and pumas exhibited multiple scales of selection during movement. We found PathSFs produced stronger regression coefficients, larger resistance values, and superior model performance than SSFs. We observed more heterogeneous surfaces when resistance was predicted in a paired framework compared with an unpaired framework. Lastly, we observed bias in habitat use and resistance results when using a GPS collar sampling interval longer than 5 min. The methods presented provide a novel way to model multi-scale habitat selection and resistance from movement data. Due to the sensitivity of resistance surfaces to method, scale, and GPS schedule, care should be used when modeling corridors for conservation purposes using these methods. C1 [Zeller, Katherine A.; McGarigal, Kevin] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Zeller, Katherine A.] Panthera, 8 West 40th St, New York, NY 10018 USA. [Cushman, Samuel A.] US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Beier, Paul] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86011 USA. [Vickers, T. Winston; Boyce, Walter M.] Univ Calif Davis, Sch Vet Med, Wildlife Hlth Ctr, Davis, CA 95616 USA. RP Zeller, KA (reprint author), Panthera, 8 West 40th St, New York, NY 10018 USA. EM kathyzeller@gmail.com FU National Science Foundation under NSF [DGE-0907995]; Kaplan Graduate Award; Nature Conservancy, Orange County Transportation Corridor Agency; Nature Reserve of Orange County; McBeth Foundation FX We thank B. Compton and E. Plunkett, for assistance with computational capacity, D. Dawn, D. Krucki, C. Bell, P. Bryant, D. Stewart, and K. Krause for field assistance. We would also like to thank the following landowners/managers: The Nature Conservancy, California Department of Fish and Wildlife, Orange County Parks Department, The New Irvine Ranch Conservancy, Audubon Starr Ranch Reserve, Riverside County Parks Department, and the Cleveland National Forest. This material is based upon work supported by the National Science Foundation under NSF DGE-0907995, a Kaplan Graduate Award, The Nature Conservancy, Orange County Transportation Corridor Agency, The Nature Reserve of Orange County, and the McBeth Foundation. NR 48 TC 5 Z9 5 U1 21 U2 30 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1319 EP 1335 DI 10.1007/s10980-015-0301-6 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300011 ER PT J AU Cushman, SA Elliot, NB Macdonald, DW Loveridge, AJ AF Cushman, Samuel A. Elliot, Nicholas B. Macdonald, David W. Loveridge, Andrew J. TI A multi-scale assessment of population connectivity in African lions (Panthera leo) in response to landscape change SO LANDSCAPE ECOLOGY LA English DT Article DE Connectivity; Fragmentation; FRAGSTATS; Resistant kernel; Scale; UNICOR ID NORTHERN ROCKY-MOUNTAINS; HABITAT LOSS; ROAD; AMPHIBIANS; CONSERVATION; CARNIVORES; DISPERSAL; MOVEMENTS; LINKAGES; FENCE AB Habitat loss and fragmentation are among the major drivers of population declines and extinction, particularly in large carnivores. Connectivity models provide practical tools for assessing fragmentation effects and developing mitigation or conservation responses. To be useful to conservation practitioners, connectivity models need to incorporate multiple scales and include realistic scenarios based on potential changes to habitat and anthropogenic pressures. This will help to prioritize conservation efforts in a changing landscape. The goal of our paper was to evaluate differences in population connectivity for lions (Panthera leo) across the Kavango-Zambezi Trans-frontier Conservation Area (KAZA) under different landscape change scenarios and a range of dispersal distances. We used an empirically optimized resistance surface, based on analysis of movement pathways of dispersing lions in southern Africa to calculate resistant kernel connectivity. We assessed changes in connectivity across nine landscape change scenarios, under each of which we explored the behavior of lions with eight different dispersal abilities. Our results demonstrate that reductions in the extent of the protected area network and/or fencing protected areas will result in large declines in the extent of population connectivity, across all modeled dispersal abilities. Creation of corridors or erection of fences strategically placed to funnel dispersers between protected areas increased overall connectivity of the population. Our results strongly suggest that the most effective means of maintaining long-term population connectivity of lions in the KAZA region involves retaining the current protected area network, augmented with protected corridors or strategic fencing to direct dispersing individuals towards suitable habitat and away from potential conflict areas. C1 [Cushman, Samuel A.] USDA Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Elliot, Nicholas B.; Macdonald, David W.; Loveridge, Andrew J.] Univ Oxford, Dept Zool, Wildlife Conservat Res Unit, Recanati Kaplan Ctr, Tubney House,Abingdon Rd, Tubney OX13 5QL, Oxon, England. RP Macdonald, DW (reprint author), Univ Oxford, Dept Zool, Wildlife Conservat Res Unit, Recanati Kaplan Ctr, Tubney House,Abingdon Rd, Tubney OX13 5QL, Oxon, England. EM david.macdonald@zoo.ox.ac.uk NR 44 TC 1 Z9 1 U1 26 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1337 EP 1353 DI 10.1007/s10980-015-0292-3 PG 17 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300012 ER PT J AU Krishnamurthy, R Cushman, SA Sarkar, MS Malviya, M Naveen, M Johnson, JA Sen, S AF Krishnamurthy, Ramesh Cushman, Samuel A. Sarkar, Mriganka S. Malviya, Manjari Naveen, Moorthy Johnson, Jeyaraj A. Sen, Subharanjan TI Multi-scale prediction of landscape resistance for tiger dispersal in central India SO LANDSCAPE ECOLOGY LA English DT Article DE Tiger; Panthera tigris; Landscape resistance; Path-level modeling; Connectivity; Scale; Resistant kernel; UNICOR ID HABITAT SELECTION; GENE FLOW; MOVEMENT BEHAVIOR; MARTES-AMERICANA; LYNX-RUFUS; CONNECTIVITY; SCALE; MODEL; DIFFERENTIATION; POPULATION AB Connectivity models for animal movement frequently use resistance surfaces, but rarely incorporate actual movement data and multiple scale drivers of landscape resistance. Using GPS data, we developed a multi-scale model of landscape resistance for tiger (Panthera tigris) dispersal in central India and evaluated the performance, interpretation and predictions against single scale models. Six dispersing tiger paths were subjected to a path level analysis with conditional logistic regression to parameterize a resistance surface. We evaluated for 21 scales of available habitat and selected the best scale for each variable. We derived a scale-optimized multivariate path selection function and predicted landscape resistance across the landscape. The tigers preferred to move along areas with forest cover at relatively high elevations along the ridges with rugged topography at broad scale, while avoiding areas with agriculture-village matrix at fine scale. We found that the scale that was most supported by Akaike's information criterion was not always the scale that maximized the magnitude (effect size) of the relationship. Further, the multi-scale optimized model differed substantially from the single scale models in terms of variable importance, magnitude of coefficients and predictions of connectivity. Our results demonstrate that the variables in landscape resistance models produce markedly different predictions of population connectivity depending on the scales of analyses and interpretation. Thus, scale optimization in parameterization is critical for appropriate inferences and sound management strategies. C1 [Krishnamurthy, Ramesh; Sarkar, Mriganka S.; Malviya, Manjari; Naveen, Moorthy; Johnson, Jeyaraj A.] Wildlife Inst India, Dehra Dun 248001, Uttarakhand, India. [Cushman, Samuel A.] USDA Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. [Sen, Subharanjan] Madhya Pradesh Forest Dept, Bhopal, Madhya Pradesh, India. RP Krishnamurthy, R (reprint author), Wildlife Inst India, Dehra Dun 248001, Uttarakhand, India. EM ramesh@wii.gov.in NR 68 TC 0 Z9 0 U1 18 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD AUG PY 2016 VL 31 IS 6 BP 1355 EP 1368 DI 10.1007/s10980-016-0363-0 PG 14 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA DR6HZ UT WOS:000380004300013 ER PT J AU Groover, A AF Groover, Andrew TI Gravitropisms and reaction woods of forest trees - evolution, functions and mechanisms SO NEW PHYTOLOGIST LA English DT Review DE compression wood; genomics; poplar; tension wood; wood development ID TENSION WOOD; COMPRESSION WOOD; SHOOT GRAVITROPISM; STRESS GENERATION; AUXIN TRANSPORT; OPPOSITE WOOD; ARABINOGALACTAN PROTEINS; GRAVITATIONAL INDUCTION; ARABIDOPSIS-THALIANA; LIGNIN DISTRIBUTION AB The woody stems of trees perceive gravity to determine their orientation, and can produce reaction woods to reinforce or change their position. Together, graviperception and reaction woods play fundamental roles in tree architecture, posture control, and reorientation of stems displaced by wind or other environmental forces. Angiosperms and gymnosperms have evolved strikingly different types of reaction wood. Tension wood of angiosperms creates strong tensile force to pull stems upward, while compression wood of gymnosperms creates compressive force to push stems upward. In this review, the general features and evolution of tension wood and compression wood are presented, along with descriptions of how gravitropisms and reaction woods contribute to the survival and morphology of trees. An overview is presented of the molecular and genetic mechanisms underlying graviperception, initial graviresponse and the regulation of tension wood development in the model angiosperm, Populus. Critical research questions and new approaches are discussed. C1 [Groover, Andrew] US Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Groover, Andrew] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. RP Groover, A (reprint author), US Forest Serv, Pacific Southwest Res Stn, Davis, CA 95618 USA.; Groover, A (reprint author), Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. EM agroover@fs.fed.us OI Groover, Andrew/0000-0002-6686-5774 FU USDA AFRI [2015-67013-22891]; DOE Office of Science, Office of Biological and Environmental Research (BER) [DE-SC0007183] FX I thank Suzanne Gerttula for images of tension wood histology and time- lapse movies, donation of Podocarpus samples, and critical reading of the draft manuscript. I thank Elisabeth Wheeler for contributions of fossil wood images. This work was supported by grants 2015-67013-22891 from the USDA AFRI and DOE Office of Science, Office of Biological and Environmental Research (BER), grant no. DE-SC0007183. NR 100 TC 1 Z9 1 U1 16 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD AUG PY 2016 VL 211 IS 3 BP 790 EP 802 DI 10.1111/nph.13968 PG 13 WC Plant Sciences SC Plant Sciences GA DR5IR UT WOS:000379937200004 PM 27111862 ER PT J AU Scafaro, AP Galle, A Van Rie, J Carmo-Silva, E Salvucci, ME Atwell, BJ AF Scafaro, Andrew P. Galle, Alexander Van Rie, Jeroen Carmo-Silva, Elizabete Salvucci, Michael E. Atwell, Brian J. TI Heat tolerance in a wild Oryza species is attributed to maintenance of Rubisco activation by a thermally stable Rubisco activase ortholog SO NEW PHYTOLOGIST LA English DT Article DE heat tolerance; Oryza australiensis; Oryza meridionalis; photosynthesis; rice (Oryza sativa); Rubisco activase (RCA); thermal stability ID RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE; MODERATELY HIGH-TEMPERATURES; RICE; PHOTOSYNTHESIS; LEAVES; STRESS; GROWTH; STABILITY; OXYGENASE; PROTEIN AB The mechanistic basis of tolerance to heat stress was investigated in Oryza sativa and two wild rice species, Oryza meridionalis and Oryza australiensis. The wild relatives are endemic to the hot, arid Australian savannah. Leaf elongation rates and gas exchange were measured during short periods of supra-optimal heat, revealing species differences. The Rubisco activase (RCA) gene from each species was sequenced. Using expressed recombinant RCA and leaf-extracted RCA, the kinetic properties of the two isoforms were studied under high temperatures. Leaf elongation was undiminished at 45 degrees C in O.australiensis. The net photosynthetic rate was almost 50% slower in O.sativa at 45 degrees C than at 28 degrees C, while in O.australiensis it was unaffected. Oryza meridionalis exhibited intermediate heat tolerance. Based on previous reports that RCA is heat-labile, the Rubisco activation state was measured. It correlated positively with leaf elongation rates across all three species and four periods of exposure to 45 degrees C. Sequence analysis revealed numerous polymorphisms in the RCA amino acid sequence from O.australiensis. The O. australiensis RCA enzyme was thermally stable up to 42 degrees C, contrasting with RCA from O.sativa, which was inhibited at 36 degrees C. We attribute heat tolerance in the wild species to thermal stability of RCA, enabling Rubisco to remain active. C1 [Scafaro, Andrew P.; Atwell, Brian J.] Macquarie Univ, Fac Sci & Engn, Dept Biol Sci, Sydney, NSW 2109, Australia. [Scafaro, Andrew P.] Australian Natl Univ, ARC Ctr Excellence Plant Energy Biol, Res Sch Biol, Canberra, ACT 0200, Australia. [Galle, Alexander; Van Rie, Jeroen] Bayer CropSci NV, Innovat Ctr Trait Res, Technol Pk 38, B-9052 Ghent, Belgium. [Carmo-Silva, Elizabete] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Salvucci, Michael E.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Atwell, BJ (reprint author), Macquarie Univ, Fac Sci & Engn, Dept Biol Sci, Sydney, NSW 2109, Australia. EM brian.atwell@mq.edu.au NR 60 TC 1 Z9 2 U1 18 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD AUG PY 2016 VL 211 IS 3 BP 899 EP 911 DI 10.1111/nph.13963 PG 13 WC Plant Sciences SC Plant Sciences GA DR5IR UT WOS:000379937200014 PM 27145723 ER PT J AU Liu, ZH Gao, YY Kim, YM Faris, JD Shelver, WL de Wit, PJGM Xu, SS Friesen, TL AF Liu, Zhaohui Gao, Yuanyuan Kim, Yong Min Faris, Justin D. Shelver, Weilin L. de Wit, Pierre J. G. M. Xu, Steven S. Friesen, Timothy L. TI SnTox1, a Parastagonospora nodorum necrotrophic effector, is a dual-function protein that facilitates infection while protecting from wheat-produced chitinases SO NEW PHYTOLOGIST LA English DT Article DE chitin; host-selective toxin; necrotroph; necrotrophic effector; Parastagonosopora nodorum; programmed cell death (PCD); wheat (Triticum aestivum) chitinases ID PYRENOPHORA-TRITICI-REPENTIS; HOST-SELECTIVE TOXINS; TERES F TERES; STAGONOSPORA-NODORUM; CLADOSPORIUM-FULVUM; PTR TOXA; BARLEY; RESISTANCE; TOMATO; SUSCEPTIBILITY AB SnTox1 induces programmed cell death and the up-regulation of pathogenesis-related genes including chitinases. Additionally, SnTox1 has structural homology to several plant chitin-binding proteins. Therefore, we evaluated SnTox1 for chitin binding and localization. We transformed an avirulent strain of Parastagonospora nodorum as well as three nonpathogens of wheat (Triticum aestivum), including a necrotrophic pathogen of barley, a hemibiotrophic pathogen of sugar beet and a saprotroph, to evaluate the role of SnTox1 in infection and in protection from wheat chitinases. SnTox1 bound chitin and an SnTox1-green fluorescent fusion protein localized to the mycelial cell wall. Purified SnTox1 induced necrosis in the absence of the pathogen when sprayed on the leaf surface and appeared to remain on the leaf surface while inducing both epidermal and mesophyll cell death. SnTox1 protected the different fungi from chitinase degradation. SnTox1 was sufficient to change the host range of a necrotrophic pathogen but not a hemibiotroph or saprotroph. Collectively, this work shows that SnTox1 probably interacts with a receptor on the outside of the cell to induce cell death to acquire nutrients, but SnTox1 accomplishes a second role in that it protects against one aspect of the defense response, namely the effects of wheat chitinases. C1 [Liu, Zhaohui; Gao, Yuanyuan; Kim, Yong Min; Friesen, Timothy L.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA. [Faris, Justin D.; Xu, Steven S.; Friesen, Timothy L.] ARS, Northern Crop Sci Lab, USDA, Fargo, ND 58102 USA. [Shelver, Weilin L.] ARS, Biosci Res Lab, USDA, Fargo, ND 58102 USA. [de Wit, Pierre J. G. M.] Wageningen Univ, Lab Phytopathol, Droevendaalsesteeg 1, NL-6708 PB Wageningen, Netherlands. RP Friesen, TL (reprint author), North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58102 USA.; Friesen, TL (reprint author), ARS, Northern Crop Sci Lab, USDA, Fargo, ND 58102 USA. EM timothy.friesen@ars.usda.gov NR 38 TC 2 Z9 2 U1 9 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD AUG PY 2016 VL 211 IS 3 BP 1052 EP 1064 DI 10.1111/nph.13959 PG 13 WC Plant Sciences SC Plant Sciences GA DR5IR UT WOS:000379937200025 PM 27041151 ER PT J AU Artlip, TS Wisniewski, ME Takatsuji, H Bassett, CL AF Artlip, Timothy S. Wisniewski, Michael E. Takatsuji, Hiroshi Bassett, Carole L. TI Engineering carpel-specific cold stress tolerance: a case study in Arabidopsis SO PHYSIOLOGIA PLANTARUM LA English DT Article ID PEACH PRUNUS-PERSICA; PRINCIPAL STORAGE PROTEINS; MOSAIC VIRUS-35S PROMOTER; DEHYDRIN GENES; DEVELOPMENTAL BIOCHEMISTRY; COTTONSEED EMBRYOGENESIS; FREEZING TOLERANCE; ACIDIC DEHYDRIN; LOW-TEMPERATURE; WATER-DEFICIT AB Climate change predictions forecast an increase in early spring frosts that could result in severe damage to perennial crops. For example, the Easter freeze of April 2007 left several states in the United States reporting a complete loss of that year's peach crop. The most susceptible organ to early frost damage in fruit trees is the carpel, particularly during bloom opening. In this study, we explored the use of a carpel-specific promoter (ZPT2-10) from petunia (Petunia hybrida var. Mitchell) to drive expression of the peach dehydrin PpDhn1. In peach, this gene is exceptionally responsive to low temperature but has not been observed to be expressed in carpels. This study examined carpel-specific properties of a petunia promoter driving the expression of the GUS gene (uidA) in transgenic Arabidopsis flowers and developed a carpel-specific ion leakage test to assess freezing tolerance. A homozygous Arabidopsis line (line 1-20) carrying the petunia ZPT2-10 promoter::PpDhn1 construct was obtained and freezing tolerance in the transgenic line was compared with an untransformed control. Overexpression of PpDhn1 in line 1-20 provided as much as a 1.9 degrees C increase in carpel freezing tolerance as measured by electrolyte leakage. C1 [Artlip, Timothy S.; Wisniewski, Michael E.] ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. [Takatsuji, Hiroshi] Natl Inst Agrobiol Sci, Dis Resistant Crops Res Unit, 2-1-2 Kannondai, Tsukuba, Ibaraki 3058602, Japan. [Bassett, Carole L.] ARS, USDA, 18933 Planters Lane, Keedysville, MD 21756 USA. RP Artlip, TS (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM Tim.Artlip@ars.usda.gov FU ARS [CRIS 1931-21220-014-00D, CRIS 1931-21000-022-00D] FX We thank Jing Ma for her design and construction of the plasmids used in this study and for her assistance with the growth, selection and analysis of the Arabidopsis lines. We also thank Dr Zongrang Liu for providing the C10 strain of Arabidopsis and for auxiliary vectors to facilitate the cloning steps. This work was supported entirely by in-house ARS funds (CRIS 1931-21220-014-00D and CRIS 1931-21000-022-00D). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 57 TC 0 Z9 0 U1 7 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD AUG PY 2016 VL 157 IS 4 BP 469 EP 478 DI 10.1111/ppl.12420 PG 10 WC Plant Sciences SC Plant Sciences GA DR7EP UT WOS:000380063100006 PM 26806544 ER PT J AU Bassi, FM Ghavami, F Hayden, MJ Wang, Y Forrest, KL Kong, S Dizon, R de Jimenez, MKM Meinhardt, SW Mergoum, M Gu, YQ Kianian, SF AF Bassi, Filippo M. Ghavami, Farhad Hayden, Matthew J. Wang, Yi Forrest, Kerrie L. Kong, Stephan Dizon, Rhoderissa de Jimenez, Monika K. Michalak Meinhardt, Steven W. Mergoum, Mohamed Gu, Yong Q. Kianian, Shahryar F. TI Fast-forward genetics by radiation hybrids to saturate the locus regulating nuclear-cytoplasmic compatibility in Triticum SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE speciation; species cytoplasm specific; fast-forward genetics; BulkSeq; synteny; wheat ID D-GENOME PROGENITOR; AEGILOPS-TAUSCHII; BIOLOGICAL FUNCTIONS; RHOMBOID PROTEASES; TETRAPLOID WHEAT; LYSINE MUTATION; CHROMOSOME 1D; SNP DISCOVERY; DURUM-WHEAT; B-GENOME AB The nuclear-encoded species cytoplasm specific (scs) genes control nuclear-cytoplasmic compatibility in wheat (genus Triticum). Alloplasmic cells, which have nucleus and cytoplasm derived from different species, produce vigorous and vital organisms only when the correct version of scs is present in their nucleus. In this study, bulks of invivo radiation hybrids segregating for the scs phenotype have been genotyped by sequencing with over 1.9million markers. The high marker saturation obtained for a critical region of chromosome 1D allowed identification of 3318 reads that mapped in close proximity of the scs. A novel in silico approach was deployed to extend these short reads to sequences of up to 70Kb in length and identify candidate open reading frames (ORFs). Markers were developed to anchor the short contigs containing ORFs to a radiation hybrid map of 650 individuals with resolution of 288Kb. The region containing the scs locus was narrowed to a single Bacterial Artificial Chromosome (BAC) contig of Aegilops tauschii. Its sequencing and assembly by nano-mapping allowed rapid identification of a rhomboid gene as the only ORF existing within the refined scs locus. Resequencing of this gene from multiple germplasm sources identified a single nucleotide mutation, which gives rise to a functional amino acid change. Gene expression characterization revealed that an active copy of this rhomboid exists on all homoeologous chromosomes of wheat, and depending on the specific cytoplasm each copy is preferentially expressed. Therefore, a new methodology was applied to unique genetic stocks to rapidly identify a strong candidate gene for the control of nuclear-cytoplasmic compatibility in wheat. C1 [Bassi, Filippo M.; Ghavami, Farhad; Dizon, Rhoderissa; de Jimenez, Monika K. Michalak; Mergoum, Mohamed; Kianian, Shahryar F.] North Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Bassi, Filippo M.] ICARDA, Rabat, Morocco. [Ghavami, Farhad] Eurofins BioDiagnost Inc, River Falls, WI USA. [Hayden, Matthew J.; Forrest, Kerrie L.; Kong, Stephan] AgriBiosci Ctr, Dept Environm & Primary Ind, Bundoora, Vic, Australia. [Wang, Yi; Gu, Yong Q.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. [Meinhardt, Steven W.] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. [Kianian, Shahryar F.] Univ Minnesota, ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Mergoum, Mohamed] Univ Georgia, Dept Crop & Soil, 1109 Expt St, Griffin, GA 30223 USA. RP Kianian, SF (reprint author), North Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA.; Kianian, SF (reprint author), Univ Minnesota, ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. EM Shahryar.Kianian@ars.usda.gov FU National Science Foundation, Plant Genome Research Program (NSF-IOS PGRP) [IOS-0822100]; scholarship "Master and Back Regione Autonoma della Sardegna"; Monsanto Beachell Borlaug International Program FX The authors wish to thank J. Hegstad and A. Peckrull for their extensive technical support, and Drs. Jaroslav Dolezel and Hana Simkova for providing access to the DNA of flow-sorted chromosomes. This research was supported by the National Science Foundation, Plant Genome Research Program (NSF-IOS PGRP) Grant No. IOS-0822100 to SFK. FMB was supported by scholarship "Master and Back Regione Autonoma della Sardegna" and "Monsanto Beachell Borlaug International Program". NR 55 TC 0 Z9 0 U1 14 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD AUG PY 2016 VL 14 IS 8 BP 1716 EP 1726 DI 10.1111/pbi.12532 PG 11 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA DR5QX UT WOS:000379958600007 PM 26915753 ER PT J AU Zhang, XH Warburton, ML Setter, T Liu, HJ Xue, YD Yang, N Yan, JB Xiao, YJ AF Zhang, Xuehai Warburton, Marilyn L. Setter, Tim Liu, Haijun Xue, Yadong Yang, Ning Yan, Jianbing Xiao, Yingjie TI Genome-wide association studies of drought-related metabolic changes in maize using an enlarged SNP panel SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; ZEA-MAYS L.; ARABIDOPSIS-THALIANA; BIOCHEMICAL INSIGHTS; TOLERANCE; IDENTIFICATION; RESPONSES; NETWORK; STRESS; KERNEL AB Genetic determinants of metabolites related to drought tolerance in maize. Water deficit or drought is one of the most serious abiotic stresses of plant development and greatly reduces crop production, and the plant's response to this deficit leads to many metabolic changes. To dissect the genetic basis of these metabolic traits in maize, we performed a genome-wide association analysis of drought-related traits using 156,599 SNPs in 318 maize inbred lines. In total, 123 significant SNP/trait associations (P a parts per thousand currency sign 6.39E-6) involving 63 loci were identified for related metabolic and physiological traits in multiple tissues and different environments under two irrigation conditions. Of the 63, 23 loci demonstrated a significant interaction effect between QTL and water status, indicating that these metabolite-associated loci were probably related to drought stress tolerance. To evaluate the potential utility of metabolite-associated loci applied in hybrid maize breeding, we assembled two groups of hybrid entries with high or low drought tolerance and measured the metabolic and physiological traits. In the hybrid pools, a set of 10 metabolite-associated loci identified in leaf and ear were validated as responsive to drought stress. The favorable alleles of these ten loci were significantly enriched in hybrids with high drought tolerance, which jointly explained almost 18.4 % of the variation in drought tolerance using a multivariate logistic regression model. These results provide clues to understanding the genetic basis of metabolic and physiological changes related to drought tolerance, potentially facilitating the genetic improvement of varieties with high drought tolerance in maize breeding programs. C1 [Zhang, Xuehai; Liu, Haijun; Yang, Ning; Yan, Jianbing; Xiao, Yingjie] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. [Warburton, Marilyn L.] Mississippi State Univ, ARS, USDA, Corn Host Plant Res Resistance Unit, Box 9555, Starkville, MS 39762 USA. [Setter, Tim] Cornell Univ, Sch Integrat Plant Sci, Sect Soil & Crop Sci, Ithaca, NY 14853 USA. [Xue, Yadong] Henan Agr Univ, Agron Coll, Zhengzhou 450002, Peoples R China. RP Xiao, YJ (reprint author), Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China. EM shanren0179@163.com FU National Hi-Tech Research and Development Program of China [2012AA10A307]; National Natural Science Foundation of China [31222041, 31401389]; Generation Challenge Program of the CGIAR FX This research was supported by the National Hi-Tech Research and Development Program of China (2012AA10A307), the National Natural Science Foundation of China (31222041, 31401389) and the Generation Challenge Program of the CGIAR. NR 45 TC 3 Z9 3 U1 18 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2016 VL 129 IS 8 BP 1449 EP 1463 DI 10.1007/s00122-016-2716-0 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR4FV UT WOS:000379857800001 PM 27121008 ER PT J AU Wang, YH VandenLangenberg, K Wehner, TC Kraan, PAG Suelmann, J Zheng, XY Owens, K Weng, YQ AF Wang, Yuhui VandenLangenberg, Kyle Wehner, Todd C. Kraan, Peter A. G. Suelmann, Jos Zheng, Xiangyang Owens, Ken Weng, Yiqun TI QTL mapping for downy mildew resistance in cucumber inbred line WI7120 (PI 330628) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID PSEUDOPERONOSPORA-CUBENSIS POPULATIONS; SATIVUS L.; DISEASE RESISTANCE; RATING-SCALES; GENOME; MARKERS; GENE; CROSSES; LINKAGE; BERK. AB Host resistance in WI7120 cucumber to prevailing downy mildew pathogen field populations is conferred by two major-effect, one moderate-effect and two minor-effect QTL. Downy mildew (DM) caused by the obligate oomycete Pseudoperonospora cubensis is the most devastating fungal disease of cucumber worldwide. The molecular mechanism of DM resistance in cucumber is poorly understood, and use of marker-assisted breeding for DM resistance is not widely available. Here, we reported QTL mapping results for DM resistance with 243 F-2:3 families from the cross between DM-resistant inbred line WI7120 (PI 330628) and susceptible '9930'. A linkage map was developed with 348 SSR and SNP markers. Phenotyping of DM inoculation responses were conducted in four field trails in 2 years at three locations. Four QTL, dm2.1, dm4.1, dm5.1, and dm6.1 were consistently and reliably detected across at least three of the four environments which together could explain 62-76 % phenotypic variations (R (2)). Among them, dm4.1 and dm5.1 were major-effect QTL (R (2) = 15-30 %) with only additive effects; dm2.1 (R (2) = 5-15 %) and dm6.1 (R (2) = 4-8 %) had moderate and minor effects, respectively. Epistatic effects were detected for dm2.1 and dm6.1 with both dm4.1 and dm5.1. One additional minor-effect QTL, dm6.2 (R (2) = 3-5 %) was only detectable with the chlorosis rating criterion. All alleles contributing to DM resistance were from WI7120. This study revealed two novel QTL for DM resistance and the unique genetic architecture of DM resistance in WI7120 conferring high level resistance to prevailing DM populations in multiple countries. The effects of disease rating scales, rating time and criteria, population size in phenotyping DM resistance on the power of QTL detection, and the use of DM resistance in WI7120 in cucumber breeding were discussed. C1 [Wang, Yuhui; Weng, Yiqun] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [VandenLangenberg, Kyle; Wehner, Todd C.] North Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Kraan, Peter A. G.; Suelmann, Jos] Bayer Vegetable Seeds, Haelen, Netherlands. [Zheng, Xiangyang; Owens, Ken] Magnum Seeds, Dixon, CA 95620 USA. [Weng, Yiqun] ARS, USDA, Vegetable Crops Res Unit, Madison, WI 53705 USA. RP Weng, YQ (reprint author), Univ Wisconsin, Dept Hort, Madison, WI 53706 USA.; Weng, YQ (reprint author), ARS, USDA, Vegetable Crops Res Unit, Madison, WI 53705 USA. EM yiqun.weng@ars.usda.gov FU US Department of Agriculture (USDA)-Specialty Crop Research Initiative Grant (SCRI) [2011-51181-30661] FX The authors thank Kristin Haider for technical help. We are also indebted to Vittorio Stravato, and Giuseppe Carannante for help phenotyping of DM resistance in the IT2013 experiment. This research was supported by the US Department of Agriculture (USDA)-Specialty Crop Research Initiative Grant (SCRI, project # 2011-51181-30661) to YW. Names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by USDA implies no approval of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 54 TC 1 Z9 1 U1 11 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2016 VL 129 IS 8 BP 1493 EP 1505 DI 10.1007/s00122-016-2719-x PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR4FV UT WOS:000379857800004 PM 27147071 ER PT J AU Gill, U Brueggeman, R Nirmala, J Chai, Y Steffenson, B Kleinhofs, A AF Gill, Upinder Brueggeman, Robert Nirmala, Jayaveeramuthu Chai, Yuan Steffenson, Brian Kleinhofs, Andris TI Molecular and genetic characterization of barley mutants and genetic mapping of mutant rpr2 required for Rpg1-mediated resistance against stem rust SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID MESSENGER-RNA STABILITY; PROTEIN-KINASE RPG1; DISEASE-RESISTANCE; POWDERY MILDEW; ARABIDOPSIS-THALIANA; GENOME; IDENTIFICATION; MUTATIONS; PATHOGEN; RECOGNITION AB This study describes the generation, screening, genetic and molecular characterization, and high-resolution mapping of barley mutants susceptible to stem rust ( Puccinia graminis f. sp. tritici ) races MCCF and HKHJ. A single gene, Rpg1, has protected barley cultivars against many races of stem rust pathogen (Puccinia graminis f. sp. tritici) for the last 70 years in the United States and Canada. To identify signaling components of protein product RPG1, we employed a mutagenesis approach. Using this approach, six mutants exhibiting susceptibility to Puccinia graminis f. sp. tritici races MCCF and HKHJ were identified in the gamma irradiated M-2 population of resistant cultivar Morex, which carries Rpg1 on chromosome 7H. The mutants retained a functional Rpg1 gene and an apparently functional protein, suggesting that the mutated genes were required for downstream or upstream signaling. Selected mutants were non-allelic, hence each mutant represents a unique gene. Low and high-resolution genetic mapping of the rpr2 mutant identified chromosome 6H (bin 6) as the location of the mutated gene. The target region was reduced to 0.6 cM and gene content analyzed. Based on the published barley genomic sequence, the target region contains approximately 157 genes, including a set that encodes putative leucine-rich receptor-like protein kinases, which may be strong candidates for the gene of interest. Overall, this study presents a strong platform for future map-based cloning of genes identified in this mutant screen. C1 [Gill, Upinder; Nirmala, Jayaveeramuthu; Kleinhofs, Andris] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Brueggeman, Robert] North Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Chai, Yuan; Steffenson, Brian] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Kleinhofs, Andris] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. [Gill, Upinder] Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA. [Nirmala, Jayaveeramuthu] ARS, Cereal Dis Lab, USDA, St Paul, MN 55108 USA. RP Gill, U; Kleinhofs, A (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA.; Kleinhofs, A (reprint author), Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA.; Gill, U (reprint author), Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA. EM ugill@noble.org; andyk@wsu.edu FU National Research Initiative of the United States Department of Agriculture, Cooperative State Research, Education and Extension Service [2007-35301-18205] FX This research was supported by the National Research Initiative of the United States Department of Agriculture, Cooperative State Research, Education and Extension Service Grant No. 2007-35301-18205 to AK and BJS. NR 46 TC 0 Z9 0 U1 15 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2016 VL 129 IS 8 BP 1519 EP 1529 DI 10.1007/s00122-016-2721-3 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR4FV UT WOS:000379857800006 PM 27142847 ER PT J AU Goettel, W Ramirez, M Upchurch, RG An, YQC AF Goettel, Wolfgang Ramirez, Martha Upchurch, Robert G. An, Yong-qiang Charles TI Identification and characterization of large DNA deletions affecting oil quality traits in soybean seeds through transcriptome sequencing analysis SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID X-RAY-IRRADIATION; PALMITIC ACID CONTENT; MOLECULAR CHARACTERIZATION; ACTIN SUBCLASS; MUTANT; EXPRESSION; INHERITANCE; GENOME; DIVERGENCE; ALLELES AB Identification and characterization of a 254-kb genomic deletion on a duplicated chromosome segment that resulted in a low level of palmitic acid in soybean seeds using transcriptome sequencing. A large number of soybean genotypes varying in seed oil composition and content have been identified. Understanding the molecular mechanisms underlying these variations is important for breeders to effectively utilize them as a genetic resource. Through design and application of a bioinformatics approach, we identified nine co-regulated gene clusters by comparing seed transcriptomes of nine soybean genotypes varying in oil composition and content. We demonstrated that four gene clusters in the genotypes M23, Jack and N0304-303-3 coincided with large-scale genome rearrangements. The co-regulated gene clusters in M23 and Jack mapped to a previously described 164-kb deletion and a copy number amplification of the Rhg1 locus, respectively. The coordinately down-regulated gene clusters in N0304-303-3 were caused by a 254-kb deletion containing 19 genes including a fatty acyl-ACP thioesterase B gene (FATB1a). This deletion was associated with reduced palmitic acid content in seeds and was the molecular cause of a previously reported nonfunctional FATB1a allele, fap (nc) . The M23 and N0304-304-3 deletions were located in duplicated genome segments retained from the Glycine-specific whole genome duplication that occurred 13 million years ago. The homoeologous genes in these duplicated regions shared a strong similarity in both their encoded protein sequences and transcript accumulation levels, suggesting that they may have conserved and important functions in seeds. The functional conservation of homoeologous genes may result in genetic redundancy and gene dosage effects for their associated seed traits, explaining why the large deletion did not cause lethal effects or completely eliminate palmitic acid in N0304-303-3. C1 [Goettel, Wolfgang; An, Yong-qiang Charles] ARS, USDA, Midwest Area, Plant Genet Res Unit,Donald Danforth Plant Sci Ct, 975 N Warson Rd, St Louis, MO 63132 USA. [Ramirez, Martha; Upchurch, Robert G.] ARS, USDA, Soybean & Nitrogen Fixat Res, 2417 Gardner Hall, Raleigh, NC 27695 USA. RP An, YQC (reprint author), ARS, USDA, Midwest Area, Plant Genet Res Unit,Donald Danforth Plant Sci Ct, 975 N Warson Rd, St Louis, MO 63132 USA. EM yong-qiang.an@ars.usda.gov FU United Soybean Board (USB) [1420-632-6607]; USDA-ARS [3622-21000-037-00] FX The authors would like to thank Rick Meyer and William Novitzky for their excellent technical assistance, data analysis and valuable comments on the manuscript, and Dr. Joe Burton for his help in pedigree analysis. The research is funded by the United Soybean Board (USB Project #: 1420-632-6607) and USDA-ARS (Project #: 3622-21000-037-00) to YQA. NR 45 TC 0 Z9 0 U1 9 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD AUG PY 2016 VL 129 IS 8 BP 1577 EP 1593 DI 10.1007/s00122-016-2725-z PG 17 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA DR4FV UT WOS:000379857800010 PM 27179525 ER PT J AU Shwiff, SA Hatch, B Anderson, A Nel, LH Leroux, K Stewart, D de Scally, M Govender, P Rupprecht, CE AF Shwiff, S. A. Hatch, B. Anderson, A. Nel, L. H. Leroux, K. Stewart, D. de Scally, M. Govender, P. Rupprecht, C. E. TI Towards Canine Rabies Elimination in KwaZulu-Natal, South Africa: Assessment of Health Economic Data SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE rabies; South Africa; disease management; economics; vaccination; human exposure ID VACCINATION; DOGS; STRATEGIES; EMERGENCE; BENEFITS; PROGRAM; COST; CITY AB Rabies remains a significant problem throughout much of the developing world. An estimated 69000 people die annually from exposure to rabies. Most of these deaths are the result of being bitten by a rabid dog. Mass vaccination campaigns targeting dogs have been implemented around the world in an attempt to control or eliminate canine rabies. We analysed the vaccination and cost data for a campaign in the KwaZulu-Natal province of South Africa; we found that the cost per dog vaccinated to be $6.61 for mass campaigns and $5.41 for local campaigns. We also estimated the cost of human post-exposure prophylaxis (PEP). The cost of PEP is approximately $64.50 on average per patient, and $333 on average for the 9% of patients who receive RIG. We also found that the districts that vaccinated the most dogs per capita experienced the highest rates of human treatment and thus had the highest PEP costs. C1 [Shwiff, S. A.; Hatch, B.; Anderson, A.] USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. [Hatch, B.] Global Alliance Rabies Control, Manhattan, KS USA. [Nel, L. H.] Univ Pretoria, Fac Nat & Agr Sci, Dept Microbiol & Plant Pathol, Pretoria, South Africa. [Leroux, K.; Stewart, D.] Allerton Vet Labs, Rabies Project, Pietermaritzburg, South Africa. [de Scally, M.] South African Vet Assoc, Pietermaritzburg, South Africa. [Govender, P.] Prov Ctr Dis Control, Pietermaritzburg, South Africa. [Rupprecht, C. E.] Ross Univ, Sch Vet Med, Basseterre, St Kitts & Nevi. RP Hatch, B (reprint author), USDA APHIS Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Brody.G.Hatch@aphis.usda.gov NR 23 TC 1 Z9 1 U1 1 U2 2 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD AUG PY 2016 VL 63 IS 4 BP 408 EP 415 DI 10.1111/tbed.12283 PG 8 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA DR4LF UT WOS:000379872300009 PM 25414096 ER PT J AU Hartshorn, JA Haavik, LJ Allison, JD Meeker, JR Johnson, W Galligan, LD Chase, KD Riggins, JJ Stephen, FM AF Hartshorn, Jessica A. Haavik, Laurel J. Allison, Jeremy D. Meeker, James R. Johnson, Wood Galligan, Larry D. Chase, Kevin D. Riggins, John J. Stephen, Fred M. TI Emergence of adult female Sirex nigricornis F. and Sirex noctilio F. (Hymenoptera: Siricidae) coincides with a decrease in daily minimum and maximum temperature SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Autumnal emergence; invasive species; monitoring; phenology; Sirex nigricornis; Sirex noctilio; Siricidae ID DEPENDENT DEVELOPMENT; UNITED-STATES; BIOLOGICAL-CONTROL; S.-NIGRICORNIS; RATE MODEL; WOODWASP; FOREST; OVIPOSITION; COLEOPTERA; PHENOLOGY AB 1 The establishment and spread of Sirex noctilio F. (Hymenoptera: Siricidae), in northeastern North America necessitates reliable monitoring methods for this alien woodwasp pest of Pinus. The native congener, Sirex nigricornis F., is common across the eastern U.S.A. and has been studied as a proxy for S. noctilio. Predicting the emergence of S. noctilio is important for guiding monitoring efforts. 2 Degree-day models have been developed to predict flight phenology of S. noctilio and S. nigricornis across eastern North America. These model parameters were tested against three years of S. nigricornis collections from two locations in Arkansas. Neither of the proposed models accurately predicted emergence. 3 The emergence of females for both S. noctilio and S. nigricornis was significantly negatively correlated with minimum and maximum temperature, indicating that emergence increases when minimum and maximum temperatures decrease. These results suggest that, as S. noctilio spreads south, where higher mean temperatures are present, it will emerge later in the season as the temperatures decline compared with the current northeastern population. 4 We propose that monitoring for the spread of S. noctilio in the southeastern U.S.A. should begin slightly before daily temperatures are expected to decline (i.e. mid-September). C1 [Hartshorn, Jessica A.; Galligan, Larry D.; Stephen, Fred M.] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. [Haavik, Laurel J.; Allison, Jeremy D.] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Haavik, Laurel J.] Univ Kansas, Dept Ecol & Evolutionary Biol, Lake Forest, IL 60045 USA. [Meeker, James R.; Johnson, Wood] US Forest Serv, USDA, Forest Hlth Protect, Pineville, LA 71360 USA. [Chase, Kevin D.; Riggins, John J.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Chase, Kevin D.] Univ Canterbury, Sch Biol Sci, Private Bag 4800, Christchurch 8140, New Zealand. RP Hartshorn, JA (reprint author), Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. EM jhartsho@uark.edu OI Haavik, Laurel/0000-0002-7749-9095 FU University of Arkansas - Division of Agriculture, Agricultural Experiment Station, Natural Resources Canada; OMNR; USDA Forest Service; Forest Health Protection; Southern Research Station FX This study was supported in part by the University of Arkansas - Division of Agriculture, Agricultural Experiment Station, Natural Resources Canada, OMNR, and grants from the USDA Forest Service, Forest Health Protection, and Southern Research Station. We are grateful to personnel in the Ozark and Ouachita National Forests for permission to trap woodwasps and for help with site establishment. We also thank David Dalzotto, Boone Hardy, Billy Bruce and Chris Steiner for their assistance with data collection and sample processing. We thank Dr Andy Mauromoustakos for statistical assistance and Dr Jean J. Turgeon for his comments and revisions. Special thanks are extended to the reviewers for their work that helped improve the manuscript. NR 53 TC 0 Z9 0 U1 2 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD AUG PY 2016 VL 18 IS 3 BP 206 EP 213 DI 10.1111/afe.12153 PG 8 WC Entomology SC Entomology GA DQ7YC UT WOS:000379423700003 ER PT J AU Cooperband, MF Stouthamer, R Carrillo, D Eskalen, A Thibault, T Cosse, AA Castrillo, LA Vandenberg, JD Rugman-Jones, PF AF Cooperband, Miriam F. Stouthamer, Richard Carrillo, Daniel Eskalen, Akif Thibault, Tim Cosse, Allard A. Castrillo, Louela A. Vandenberg, John D. Rugman-Jones, Paul F. TI Biology of two members of the Euwallacea fornicatus species complex (Coleoptera: Curculionidae: Scolytinae), recently invasive in the USA, reared on an ambrosia beetle artificial diet SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Artificial rearing; cold tolerance; Fusarium dieback; insect diet; local mate competition; polyphagous shot hole borer; sex ratio; tea shot hole borer ID SHOT-HOLE BORER; COLD TOLERANCE; SEX-RATIOS; DENDROCTONUS-PONDEROSAE; STRUCTURED POPULATIONS; XYLEBORUS FERRUGINEUS; SEMIARTIFICIAL DIET; SYMBIOTIC FUNGUS; LOW-TEMPERATURES; BARK BEETLE AB 1 Recent molecular studies have found that the ambrosia beetle Euwallacea fornicatus Eichhoff (Coleoptera: Curculionidae: Scolytinae) is a complex of cryptic species, each carrying a different species of symbiotic fungus, in the genus Fusarium, which they farm within galleries inside woody hosts. Several of these beetle species have become invasive pests around the world for attacking and infecting healthy trees with their phytopathogenic fungal symbionts. 2 Diet and rearing protocols were developed for two members of the E. fornicatus species complex, polyphagous shot hole borer (PSHB) and tea shot hole borer (TSHB), using sawdust from host trees, allowing collection of data on beetle biology, phenology and sex ratios. Adults developed within 22 days at 24 degrees C. Single PSHB or TSHB foundresses averaged 32.4 and 24.7 adult female offspring, respectively, and up to 57 and 68 female adults within 6-7 weeks. A strong predictor of the number of offspring in a colony was the number of entry holes. Average sex ratios (% male) for PSHB and TSHB, respectively, were 7.4% and 7.2%. 3 Being haplodiploid, virgin PSHB foundresses were able to produce and mate with male offspring, then subsequently produce female offspring, confirming that they have arrhenotokous reproduction. 4 A cold tolerance study found significant mortality rates among PSHB colonies exposed to -5 degrees or -1 degrees C but not colonies exposed to 0 degrees, 1 degrees or 5 degrees C. 5 Given Hamilton's local mate competition (LMC) theory, a number of LMC predictions were violated. PSHB sex ratios were not affected by the number of foundresses; approximately 14% of broods did not contain males; males did not usually eclose before females but eclosed around the same time (22-23 days); and PSHB males were found walking outside of their natal galleries on the trunk of a heavily infested tree in the field. Alternatives to LMC are considered, such as early forms of sociality (maternal care, cooperative brood care), local resource enhancement and kin selection. C1 [Cooperband, Miriam F.] APHIS, Otis Lab, USDA, PPQ,CPHST, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. [Stouthamer, Richard; Rugman-Jones, Paul F.] Univ Calif Riverside, Dept Entomol, 900 Univ Ave, Riverside, CA 92521 USA. [Carrillo, Daniel] Univ Florida, IFAS, Trop Res & Educ Ctr, 18905 SW 280 ST, Homestead, FL 33031 USA. [Eskalen, Akif] Univ Calif Riverside, Dept Plant Pathol & Microbiol, 3401 Watkins Ave, Riverside, CA 92521 USA. [Thibault, Tim] Art Collect, Huntington Lib, 1151 Oxford Rd, San Marino, CA 91108 USA. [Thibault, Tim] Bot Gardens, 1151 Oxford Rd, San Marino, CA 91108 USA. [Cosse, Allard A.] ARS, USDA, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. [Castrillo, Louela A.] Cornell Univ, Dept Entomol, 129 Garden Ave, Ithaca, NY 14853 USA. [Vandenberg, John D.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. RP Cooperband, MF (reprint author), APHIS, Otis Lab, USDA, PPQ,CPHST, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. EM miriam.f.cooperband@aphis.usda.gov NR 58 TC 1 Z9 2 U1 14 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD AUG PY 2016 VL 18 IS 3 BP 223 EP 237 DI 10.1111/afe.12155 PG 15 WC Entomology SC Entomology GA DQ7YC UT WOS:000379423700005 ER PT J AU LaHue, GT Chaney, RL Adyiento-Borbe, MA Linguist, BA AF LaHue, Gabriel T. Chaney, Rufus L. Adyiento-Borbe, Maria Arlene Linguist, Bruce A. TI Alternate wetting and drying in high yielding direct-seeded rice systems accomplishes multiple environmental and agronomic objectives SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Arsenic; Global warming potential; Intermittent irrigation; Methane; Nitrous oxide; Rice ID NITROUS-OXIDE EMISSIONS; WATER-SAVING IRRIGATION; GREENHOUSE-GAS EMISSIONS; METHANE EMISSIONS; FLOODED RICE; SOIL-WATER; ORGANIC-MATTER; LOWLAND RICE; MANAGEMENT; FIELDS AB Rice (Oryza sativa L.) cultivation is critically important for global food security, yet it also represents a significant fraction of agricultural greenhouse gas (GHG) emissions and water resource use. Alternate wetting and drying (AWD) of rice fields has been shown to reduce both methane (CH4) emissions and water use, but its effect on grain yield is variable. In this three-year study we measured CH4 and nitrous oxide (N2O) emissions, rice grain total arsenic (As) concentrations, yield response to N rate, and grain yield from two AWD treatments (drill-seeded and water-seeded) and a conventionally managed water seeded treatment (control). Grain yields (average= 10 Mg ha(-1)) were similar or higher in the AWD treatments compared to the control and required similar or lower N rates to achieve these yields. Furthermore, AWD reduced growing season CH4 emissions by 60-87% while maintaining low annual N2O emissions (average = 0.38 kg N2O-N ha(-1)); N2O emissions accounted for <15% of the annual global warming potential (GWP) in all treatments. Fallow season emissions did not vary by treatment and accounted for 22-53% of annual CH4 emissions and approximately one third of annual GWP on average. The AWD treatments reduced annual GWP by 57-74% and growing season yield-scaled GWP by 59-88%. Milled grain total As, which averaged 0.114 mg kg(-1) in the control, was reduced by 59-65% in the AWD treatments. These results show that AWD has the potential to mitigate GHG emissions associated with rice cultivation and reduce rice grain total As concentrations without sacrificing grain yield or requiring higher N inputs; however future research needs to focus on adapting AWD to field scales if adoption of thiS technology is to be realized. (C) 2016 Elsevier B.V. All rights reserved. C1 [LaHue, Gabriel T.; Linguist, Bruce A.] Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. [Chaney, Rufus L.] USDA, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Adyiento-Borbe, Maria Arlene] USDA ARS, Delta Water Management Res Unit, Jonesboro, AR 72401 USA. RP LaHue, GT (reprint author), Univ Calif Davis, Dept Plant Sci, One Shields Ave, Davis, CA 95616 USA. EM gtlahue@ucdavis.edu FU California Rice Research Board; UC Davis Department of Plant Sciences FX We would like to thank the California Rice Research Board for funding this research and the UC Davis Department of Plant Sciences for funding G. T. LaHue with a Departmental Graduate Student Research Assistantship. We would furthermore like to thank Cesar Abrenilla, John Stogsdill, Kyle Anderson, and Matt Espe for their help with data collection, field management, and statistical analysis, as well as the entire UC Davis Agroecosystems lab, and Amy Poet and Carrie E. Green of the ARS lab in Beltsville, MD. NR 59 TC 3 Z9 3 U1 26 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD AUG 1 PY 2016 VL 229 BP 30 EP 39 DI 10.1016/j.agee.2016.05.020 PG 10 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA DQ7GF UT WOS:000379374200004 ER PT J AU Magunda, F Thompson, CW Schneider, DA Noh, SM AF Magunda, Forgivemore Thompson, Chelsea Wright Schneider, David A. Noh, Susan M. TI Anaplasma marginale Actively Modulates Vacuolar Maturation during Intracellular Infection of Its Tick Vector, Dermacentor andersoni SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RAB-GTPASES; MECHANISMS; PROTEINS; PHAGOCYTOPHILUM; RICKETTSIALES; IXODIDAE; ACARI; TRANSMISSION; CATTLE; CELL AB Tick-borne transmission of bacterial pathogens in the order Rickettsiales is responsible for diverse infectious diseases, many of them severe, in humans and animals. Transmission dynamics differ among these pathogens and are reflected in the pathogen-vector interaction. Anaplasma marginale has been shown to establish and maintain infectivity within Dermacentor spp. for weeks to months while escaping the complex network of vacuolar peptidases that are responsible for digestion of the tick blood meal. How this prolonged maintenance of infectivity in a potentially hostile environment is achieved has been unknown. Using the natural vector Dermacentor andersoni, we demonstrated that A. marginale-infected tick vacuoles (AmVs) concurrently recruit markers of the early endosome (Rab5), recycling endosome (Rab4 and Rab11), and late endosome (Rab7), are maintained near neutral pH, do not fuse with lysosomes, exclude the protease cathepsin L, and engage the endoplasmic reticulum and Golgi apparatus for up to 21 days postinfection. Maintenance of this safe vacuolar niche requires active A. marginale protein synthesis; in its absence, the AmVs mature into acidic, protease-active phagolysosomes. Identification of this bacterially directed modeling of the tick midgut endosome provides a mechanistic basis for examination of the differences in transmission efficiency observed among A. marginale strains and among vector populations. IMPORTANCE Ticks transmit a variety of intracellular bacterial pathogens that cause significant diseases in humans and animals. For successful transmission, these bacterial pathogens must first gain entry into the tick midgut digestive cells, avoid digestion, and establish a replicative niche without harming the tick vector. Little is known about how this replicative niche is established and maintained. Using the ruminant pathogen A. marginale and its natural tick vector, D. andersoni, this study characterized the features of the A. marginale niche in the tick midgut and demonstrates that A. marginale protein synthesis is required for the maintenance of this niche. This work opens a new line of inquiry about the pathogen effectors and their targets within the tick that mediate tick-pathogen interactions and ultimately serve as the determinants of pathogen success. C1 [Magunda, Forgivemore; Schneider, David A.; Noh, Susan M.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [Magunda, Forgivemore; Schneider, David A.; Noh, Susan M.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Magunda, Forgivemore; Noh, Susan M.] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. [Thompson, Chelsea Wright] Old Dominion Univ, Dept Biol Sci, Norfolk, VA 23529 USA. RP Magunda, F (reprint author), ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA.; Magunda, F (reprint author), Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA.; Magunda, F (reprint author), Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA. EM magundaf@vetmed.wsu.edu FU HHS \ National Institutes of Health (NIH) [R37 AI44005]; USDA \ Agricultural Research Service (ARS) [5348-32000-033-00D] FX This work, including the efforts of Susan M. Noh, was funded by HHS vertical bar National Institutes of Health (NIH) (R37 AI44005). This work, including the efforts of Susan M. Noh, was funded by USDA vertical bar Agricultural Research Service (ARS) (5348-32000-033-00D). NR 45 TC 0 Z9 0 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD AUG PY 2016 VL 82 IS 15 BP 4715 EP 4731 DI 10.1128/AEM.01030-16 PG 17 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA DR2ZK UT WOS:000379772200024 PM 27235428 ER PT J AU Wang, X Ma, MG Liu, ZL Xiang, QJ Li, X Liu, N Zhang, XP AF Wang, Xu Ma, Menggen Liu, Z. Lewis Xiang, Quanju Li, Xi Liu, Na Zhang, Xiaoping TI GRE2 from Scheffersomyces stipitis as an aldehyde reductase contributes tolerance to aldehyde inhibitors derived from lignocellulosic biomass SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Aldehyde inhibitors; Aldehyde reductase; Detoxification; Ethanol; Lignocellulosic biomass; Scheffersomyces stipitis ID XYLOSE-FERMENTING YEAST; SACCHAROMYCES-CEREVISIAE; PICHIA-STIPITIS; FERMENTATION INHIBITORS; DEGRADATION-PRODUCTS; CANDIDA-SHEHATAE; QRT-PCR; DETOXIFICATION; CONVERSION; ETHANOL AB Scheffersomyces (Pichia) stipitis is one of the most promising yeasts for industrial bioethanol production from lignocellulosic biomass. S. stipitis is able to in situ detoxify aldehyde inhibitors (such as furfural and 5-hydroxymethylfurfural (HMF)) to less toxic corresponding alcohols. However, the reduction enzymes involved in this reaction remain largely unknown. In this study, we reported that an uncharacterized open reading frame PICST_72153 (putative GRE2) from S. stipitis was highly induced in response to furfural and HMF stresses. Overexpression of this gene in Saccharomyces cerevisiae improved yeast tolerance to furfural and HMF. GRE2 was identified as an aldehyde reductase which can reduce furfural to FM with either NADH or NADPH as the co-factor and reduce HMF to FDM with NADPH as the co-factor. This enzyme can also reduce multiple aldehydes to their corresponding alcohols. Amino acid sequence analysis indicated that it is a member of the subclass "intermediate" of the short-chain dehydrogenase/reductase (SDR) superfamily. Although GRE2 from S. stipitis is similar to GRE2 from S. cerevisiae in a three-dimensional structure, some differences were predicted. GRE2 from S. stipitis forms loops at D133-E137 and T143-N145 locations with two alpha-helices at E154-K157 and E252-A254 locations, different GRE2 from S. cerevisiae with an alpha-helix at D133-E137 and a beta-sheet at T143-N145 locations, and two loops at E154-K157 and E252-A254 locations. This research provided guidelines for the study of other SDR enzymes from S. stipitis and other yeasts on tolerant mechanisms to aldehyde inhibitors derived from lignocellulosic biomass. C1 [Wang, Xu; Ma, Menggen; Zhang, Xiaoping] Sichuan Agr Univ, Dept Appl Microbiol, Coll Resources, 211 Huimin Rd, Wenjiang 611130, Sichuan, Peoples R China. [Xiang, Quanju] Sichuan Agr Univ, Inst Nat Resources & Geog Informat Technol, Coll Resources, 211 Huimin Rd, Wenjiang 611130, Sichuan, Peoples R China. [Liu, Z. Lewis] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. [Li, Xi] Sichuan Agr Univ, Coll Landscape Architecture, 211 Huimin Rd, Wenjiang 611130, Sichuan, Peoples R China. [Liu, Na] Sichuan Agr Univ, Coll Agr, Dept Plant Pathol, Wenjiang 611130, Sichuan, Peoples R China. RP Ma, MG; Zhang, XP (reprint author), Sichuan Agr Univ, Dept Appl Microbiol, Coll Resources, 211 Huimin Rd, Wenjiang 611130, Sichuan, Peoples R China.; Liu, ZL (reprint author), ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM mgen@sicau.edu.cn; ZLewis.Liu@ars.usda.gov; zhangxiaopingphd@126.com FU National Natural Science Foundation of China [31570086]; Sichuan Agricultural University [01426100] FX This work was supported by the National Natural Science Foundation of China (No. 31570086) and the Talent Introduction Fund of Sichuan Agricultural University (No. 01426100). NR 51 TC 0 Z9 0 U1 9 U2 12 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD AUG PY 2016 VL 100 IS 15 BP 6671 EP 6682 DI 10.1007/s00253-016-7445-4 PG 12 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA DQ6LU UT WOS:000379317300013 PM 27003269 ER PT J AU Childress, CJ Fuller, SA Rawles, SD Beck, BH Gaylord, TG Barrows, FT Mcentire, ME AF Childress, C. J. Fuller, S. A. Rawles, S. D. Beck, B. H. Gaylord, T. G. Barrows, F. T. Mcentire, M. E. TI Lysine supplementation of commercial fishmeal-free diet in hybrid striped bass Morone chrysops x M-saxatilis affects expression of growth-related genes SO AQUACULTURE NUTRITION LA English DT Article DE hybrid striped bass; ideal protein; lysine supplementation; myogenin; myostatin ID TROUT ONCORHYNCHUS-MYKISS; CATFISH ICTALURUS-PUNCTATUS; PLANT-BASED DIETS; SEA-BASS; MUSCLE GROWTH; AMINO-ACIDS; PROTEIN; DIFFERENTIATION; EFFICIENCY; NUTRITION AB Our recent results in hybrid striped bass (HSB) concluded that ideal protein theory accurately predicts first-limiting amino acids in commercial diet formulations if accurate amino acid availability data are used and that appropriate levels of supplemental lysine are needed to improve fish performance from fishmeal-free diets. Our goal in this study was to elucidate how dietary lysine supplementation of a commercial fishmeal-free diet influences the expression of two genes, myostatin and myogenin, controlling myogenesis in differentially growing groups of HSB. Real-time RT-PCR results in HSB suggest that the levels of lysine added to the diet (17.8, 35.1, 51.0 g kg(-1) of diet) has an impact on myogenin relative to the basal unsupplemented diet, but no effect on myostatin. Moreover, our data further suggest that the amount of dietary lysine supplementation influenced the ratio of myostatin/myogenin expression in HSB and that this pattern mimicked that of most of the growth, composition of growth and nutrient retention data from our previous study and may therefore be a useful marker for selecting fish for improved growth performance. C1 [Childress, C. J.; Fuller, S. A.; Rawles, S. D.; Beck, B. H.; Mcentire, M. E.] ARS, USDA, HKDSNARC, POB 1050,2955 Highway 130 East, Stuttgart, AR 72160 USA. [Gaylord, T. G.] US Fish & Wildlife Serv, US Dept Interior, Bozeman Fish Technol Ctr, Bozeman, MT USA. [Barrows, F. T.] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Hagerman Fish Culture Expt Stn, Hagerman, ID USA. RP Rawles, SD (reprint author), ARS, USDA, HKDSNARC, POB 1050,2955 Highway 130 East, Stuttgart, AR 72160 USA. EM steven.rawles@ars.usda.gov FU USDA/ARS [6225-31630-006-00D]; ARS Headquarters FX We thank USDA/ARS personnel R. Jacobs, T. Bader, M. Barnett, B. Farmer, G. O'Neal and J. Frost for their efforts in this work. This work was made possible by the USDA/ARS under project number 6225-31630-006-00D and an ARS Headquarters funded Post-doctoral Research Associate Program. USDA is an equal opportunity provider and employer. Mentioning of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 34 TC 0 Z9 0 U1 5 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1353-5773 EI 1365-2095 J9 AQUACULT NUTR JI Aquac. Nutr. PD AUG PY 2016 VL 22 IS 4 BP 738 EP 744 DI 10.1111/anu.12300 PG 7 WC Fisheries SC Fisheries GA DQ7XJ UT WOS:000379421300002 ER PT J AU Rodriguez-Celma, J Ceballos-Laita, L Grusak, MA Abadia, J Lopez-Millan, AF AF Rodriguez-Celma, Jorge Ceballos-Laita, Laura Grusak, Michael A. Abadia, Javier Lopez-Millan, Ana-Flor TI Plant fluid proteomics: Delving into the xylem sap, phloem sap and apoplastic fluid proteomes SO BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS LA English DT Review DE Apoplastic fluid; Phloem sap; Proteome; Xylem sap ID PLASMA-MEMBRANE PROTEOME; BRASSICA-NAPUS; ARABIDOPSIS-THALIANA; LEAF APOPLAST; MASS-SPECTROMETRY; MESOPHYLL-CELLS; STRESS-RESPONSE; GLYCINE-MAX; SALT STRESS; IN-VIVO AB The phloem sap, xylem sap and apoplastic fluid play key roles in long and short distance transport of signals and nutrients, and act as a barrier against local and systemic pathogen infection. Among other components, these plant fluids contain proteins which are likely to be important players in their functionalities. However, detailed information about their proteomes is only starting to arise due to the difficulties inherent to the collection methods. This review compiles the proteomic information available to date in these three plant fluids, and compares the proteomes obtained in different plant species in order to shed light into conserved functions in each plant fluid. Inter-species comparisons indicate that all these fluids contain the protein machinery for self maintenance and defense, including proteins related to cell wall metabolism, pathogen defense, proteolysis, and redox response. These analyses also revealed that proteins may play more relevant roles in signaling in the phloem sap and apoplastic fluid than in the xylem sap. A comparison of the proteomes of the three fluids indicates that although functional categories are somewhat similar, proteins involved are likely to be fluid-specific, except for a small group of proteins present in the three fluids, which may have a universal role, especially in cell wall maintenance and defense. This article is part of a Special Issue entitled: Plant Proteomics-a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock. (C) 2016 Elsevier B.V. All rights reserved. C1 [Rodriguez-Celma, Jorge] Univ East Anglia, John Innes Ctr, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England. [Ceballos-Laita, Laura; Abadia, Javier; Lopez-Millan, Ana-Flor] CSIC, Aula Dei Expt Stn, Dept Plant Nutr, POB 13034, E-50080 Zaragoza, Spain. [Grusak, Michael A.; Lopez-Millan, Ana-Flor] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. RP Lopez-Millan, AF (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM lopezmil@bcm.edu RI Abadia, Javier/B-8804-2008; Rodriguez-Celma, Jorge/E-9513-2012; Mangan, Rachel/A-8824-2008 OI Abadia, Javier/0000-0001-5470-5901; Rodriguez-Celma, Jorge/0000-0002-7967-5136; Mangan, Rachel/0000-0002-8788-9214 FU Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2012-31988, AGL2013-42175-R]; Spanish Ministry of Economy and Competitiveness (FEDER); Aragon Government (group A03); USDA Agricultural Research Service [58-6250-D-008]; European Union [655043]; FPI-MINECO contract FX Study supported by the Spanish Ministry of Economy and Competitiveness (MINECO projects AGL2012-31988 and AGL2013-42175-R, co-financed with FEDER) and the Aragon Government (group A03) and the USDA Agricultural Research Service (Cooperative Agreement Number 58-6250-D-008). J.R.-C. was supported by the European Union's Horizon 2020 Research and Innovation program under the Marie Sklodowska-Curie grant agreement No. 655043. L.C.-L. was supported by a FPI-MINECO contract. We thank the two anonymous reviewers for their thorough reviews. NR 94 TC 2 Z9 2 U1 21 U2 36 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1570-9639 EI 0006-3002 J9 BBA-PROTEINS PROTEOM JI BBA-Proteins Proteomics PD AUG PY 2016 VL 1864 IS 8 SI SI BP 991 EP 1002 DI 10.1016/j.bbapap.2016.03.014 PG 12 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA DQ5WN UT WOS:000379276000012 PM 27033031 ER PT J AU Kohler, GR Wallin, KF Ross, DW AF Kohler, G. R. Wallin, K. F. Ross, D. W. TI Seasonal phenology and abundance of Leucopis argenticollis, Leucopis piniperda (Diptera: Chamaemyiidae), Laricobius nigrinus (Coleoptera: Deridontidae) and Adelges tsugae (Hemiptera: Adelgidae) in the Pacific Northwest USA SO BULLETIN OF ENTOMOLOGICAL RESEARCH LA English DT Article DE hemlock woolly adelgid; silver flies; biological control ID HEMLOCK; PREY AB Adelges tsugae infested western hemlock trees were sampled periodically for 1 year at two locations in Oregon and Washington to compare the phenology and abundance of three associated predators (Leucopis argenticollis, Leucopis piniperda, and Laricobius nigrinus) and their host. On each sample date, two 3-10 cm long terminal twigs were collected from each tree and brought to the laboratory to count all life stages of A. tsugae and the three predators. Peak larval abundance of Leucopis spp. and La. nigrinus coincided with the presence of A. tsugae adults and eggs. Leucopis spp. larvae were present for a much longer period of time than were La. nigrinus larvae. Furthermore, Leucopis spp. larvae were present during both the progrediens and sistens egg stages, while La. nigrinus larvae were only present during the progrediens egg stage. Overall, we collected 2.3-3.5 times more Leucopis spp. of all life stages than La. nigrinus. These results support the continued study of Leucopis spp. from the Pacific Northwest as biological control agents for A. tsugae in the Eastern USA. C1 [Kohler, G. R.; Wallin, K. F.; Ross, D. W.] Oregon State Univ, Dept Forest Sci, Peavy Hall 154, Corvallis, OR 97331 USA. [Kohler, G. R.] Washington State Dept Nat Resources, 1111 Washington St SE, Olympia, WA 98504 USA. [Wallin, K. F.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Wallin, K. F.] US Forest Serv, USDA, Northern Res Stn, 321 Aiken Ctr, Burlington, VT 05405 USA. [Ross, D. W.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. RP Ross, DW (reprint author), Oregon State Univ, Dept Forest Sci, Peavy Hall 154, Corvallis, OR 97331 USA.; Ross, DW (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM darrell.ross@oregonstate.edu FU USDA Forest Service, Forest Health Technology Enterprise Team (FHTET), Morgantown, WV; Oregon State University College of Forestry; Oregon Sports Lottery Scholarship Fund FX The authors thank the staff at the Oregon Department of Forestry J.E. Schroeder Seed Orchard and the Thurston County Title Company for cooperation in establishing and maintaining field sites. This research was funded in part by the USDA Forest Service, Forest Health Technology Enterprise Team (FHTET), Morgantown, WV. This research was also supported by the Oregon State University College of Forestry and the Oregon Sports Lottery Scholarship Fund. NR 16 TC 1 Z9 1 U1 3 U2 5 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-4853 EI 1475-2670 J9 B ENTOMOL RES JI Bull. Entomol. Res. PD AUG PY 2016 VL 106 IS 4 BP 546 EP 550 DI 10.1017/S0007485316000250 PG 5 WC Entomology SC Entomology GA DQ7WG UT WOS:000379418000015 PM 27087639 ER PT J AU Rand, TA Morrill, WL Runyon, JB Hoelmer, KA Shanower, TG Littlefield, JL Weaver, DK AF Rand, Tatyana A. Morrill, Wendell L. Runyon, Justin B. Hoelmer, Kim A. Shanower, Thomas G. Littlefield, Jeffrey L. Weaver, David K. TI Assessing phenological synchrony between the Chinese sawfly, Cephus fumipennis (Hymenoptera: Cephidae), its egg-larval parasitoid, Collyria catoptron (Hymenoptera: Ichneumonidae), and the North American sawfly, Cephus cinctus: implications for biological control SO CANADIAN ENTOMOLOGIST LA English DT Article ID BRACON-LISSOGASTER HYMENOPTERA; WHEAT-STEM SAWFLIES; CONTROL AGENT; WINTER-WHEAT; MONTANA; FIELDS; PHOTOSYNTHESIS; POPULATIONS AB Many pest and beneficial insects overwinter as larvae in a state of diapause, with development resuming in the spring. In these cases, rates of post-diapause development of parasitoids must be synchronised with the vulnerable life stages of their hosts. Phenological asynchrony between introduced parasitoids and their targeted hosts has limited the success of some biological control efforts. Here, we assess the potential synchrony between Collyria catoptron Wahl (Hymenoptera: Ichneumonidae), a parasitoid of the Chinese wheat stem sawfly, Cephus fumipennis Eversmann (Hymenoptera: Cephidae), which is being considered as a biological control against a novel host species, Cephus cinctus Norton, in North America. We compared development timing and emergence patterns of both native and exotic species of sawflies with that of the parasitoid. We found that the mean number of days between termination of larval diapause and adult eclosion varied by less than one day across species, and patterns of emergence were also similar. The rate of development of this egg-larval parasitoid was within the range necessary to attack C. cinctus eggs. Furthermore, the development of C. cinctus from westernMontana, United States of Americamost closely matched that of the parasitoid, suggesting western Montana as a possible release area. C1 [Rand, Tatyana A.; Shanower, Thomas G.] ARS, Northern Plains Agr Res Lab, USDA, Sidney, MT 59270 USA. [Morrill, Wendell L.; Runyon, Justin B.; Littlefield, Jeffrey L.; Weaver, David K.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Hoelmer, Kim A.] ARS, USDA, European Biol Control Lab, Montferrier Sur Lez, France. [Runyon, Justin B.] US Forest Serv, Rocky Mt Res Stn, USDA, 1648 S 7th Ave, Bozeman, MT 59717 USA. [Hoelmer, Kim A.] ARS, USDA, Beneficial Insects Introduct Res, 501 S Chapel St, Newark, DE 19713 USA. [Shanower, Thomas G.] ARS, USDA, Pacific West Area Off, Albany, CA 94710 USA. RP Rand, TA (reprint author), ARS, Northern Plains Agr Res Lab, USDA, Sidney, MT 59270 USA. EM tatyana.rand@ars.usda.gov FU Montana Wheat and Barley Committee; Montana Agricultural Experiment Station FX The authors thank Hongyin Chen (United States Department of Agriculture-Agricultural Research Service; Sino-American Biological Control Laboratory, Beijing) and Aiping Liu (Chinese Academy of Agricultural Sciences, Grasslands Research Institute, Hohhot, Inner Mongolia) for their assistance in obtaining the samples from China, and J. Zhu (Penn State) for help with the statistical analysis. Norma Irish provided valuable technical assistance. Research conducted at Montana State University was supported by the Montana Wheat and Barley Committee and the Montana Agricultural Experiment Station. NR 55 TC 0 Z9 0 U1 6 U2 10 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD AUG PY 2016 VL 148 IS 4 BP 482 EP 492 DI 10.4039/tce.2015.64 PG 11 WC Entomology SC Entomology GA DR0MI UT WOS:000379601200011 ER PT J AU Mueller, KE Blumenthal, DM Pendall, E Carrillo, Y Dijkstra, FA Williams, DG Follett, RF Morgan, JA AF Mueller, K. E. Blumenthal, D. M. Pendall, E. Carrillo, Y. Dijkstra, F. A. Williams, D. G. Follett, R. F. Morgan, J. A. TI Impacts of warming and elevated CO2 on a semi-arid grassland are non-additive, shift with precipitation, and reverse over time SO ECOLOGY LETTERS LA English DT Article DE Artemisia frigida; Bouteloua gracilis; C-3 grass; C-4 grass; climate change; forb; nitrogen; plant productivity; root biomass; soil moisture ID PROGRESSIVE NITROGEN LIMITATION; PLANT COMMUNITY COMPOSITION; ATMOSPHERIC CARBON-DIOXIDE; CLIMATE-CHANGE; C-4 GRASSES; INTERANNUAL VARIABILITY; TERRESTRIAL ECOSYSTEMS; CONCEPTUAL-FRAMEWORK; WATER AVAILABILITY; SHORTGRASS STEPPE AB It is unclear how elevated CO2 (eCO(2)) and the corresponding shifts in temperature and precipitation will interact to impact ecosystems over time. During a 7-year experiment in a semi-arid grassland, the response of plant biomass to eCO(2) and warming was largely regulated by interannual precipitation, while the response of plant community composition was more sensitive to experiment duration. The combined effects of eCO(2) and warming on aboveground plant biomass were less positive in wet' growing seasons, but total plant biomass was consistently stimulated by similar to 25% due to unique, supra-additive responses of roots. Independent of precipitation, the combined effects of eCO(2) and warming on C-3 graminoids became increasingly positive and supra-additive over time, reversing an initial shift toward C-4 grasses. Soil resources also responded dynamically and non-additively to eCO(2) and warming, shaping the plant responses. Our results suggest grasslands are poised for drastic changes in function and highlight the need for long-term, factorial experiments. C1 [Mueller, K. E.; Blumenthal, D. M.; Morgan, J. A.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. [Pendall, E.; Carrillo, Y.] Univ Western Sydney, Hawkesbury Inst Environm, Penrith, NSW 2751, Australia. [Dijkstra, F. A.] Univ Sydney, Ctr Carbon Water & Food, Fac Agr & Environm, Sch Life & Environm Sci, Sydney, NSW 2006, Australia. [Williams, D. G.] Univ Wyoming, Dept Bot, Laramie, WY 82071 USA. [Follett, R. F.] ARS, Soil Plant & Nutrient Res Unit, USDA, Ft Collins, CO 80526 USA. RP Mueller, KE (reprint author), ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. EM kevin.e.mueller@gmail.com FU Climate Change, Soils & Emissions Program of the USDA-ARS; Soil Processes program of the USDA-CSREES [2008-35107-18655]; Terrestrial Ecosystem Science program of the United States Department of Energy Office of Science [DE-SC0006973]; Western Regional Center of the National Institute for Climatic Change Research; National Science Foundation [DEB 1021559] FX This research was supported by the Climate Change, Soils & Emissions Program of the USDA-ARS, the Soil Processes program of the USDA-CSREES (2008-35107-18655), the Terrestrial Ecosystem Science program of the United States Department of Energy Office of Science (DE-SC0006973), the Western Regional Center of the National Institute for Climatic Change Research, and the National Science Foundation (DEB 1021559). Dan LeCain led the initiation and maintenance of the experiment, sampling, and processing and archiving of data. Franco Miglietta and Bruce Kimball provided advice and help on the installation of the FACE and infrared heating systems, respectively. Samantha Ewers assisted with data archiving. Many people contributed to experimental design, sample collection, and sample processing, especially: J. Bell, C. Brooks, J. Bushey, M. Carlson, A. Eden, C. Ellis, A. Hansen, E. Hardy, J. Kray, L. Macdonald, L. Mancini, H. Munn, M. Nix, J. Newcomb, K. Oles, S. Raut, N. Reese, C. Rumsey, D. Smith, S. Tamang, and S. Wetherelt. NR 82 TC 2 Z9 2 U1 46 U2 64 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD AUG PY 2016 VL 19 IS 8 BP 956 EP 966 DI 10.1111/ele.12634 PG 11 WC Ecology SC Environmental Sciences & Ecology GA DR5TW UT WOS:000379966300014 PM 27339693 ER PT J AU Byers, BE Akresh, ME King, DI AF Byers, Bruce E. Akresh, Michael E. King, David I. TI Song and Male Quality in Prairie Warblers SO ETHOLOGY LA English DT Article DE birdsong; song performance; male quality; prairie warbler; Setophaga discolor ID REPRODUCTIVE SUCCESS; SEXUAL SELECTION; MARKED ANIMALS; MATE CHOICE; EVOLUTION; SURVIVAL; MODELS; BIRDS; PERFORMANCE; COMPETITION AB To determine if the songs of male prairie warblers could potentially reveal to female listeners information about the quality of singers, we compared various aspects of prairie warbler song structure and performance to attributes that might reflect a male singer's potential to enhance the fitness of his mate. We found that all the tested male attributes-arrival date, age, body size, annual survival, and fledging success-were associated with singing, most with multiple aspects of singing. Several of the song traits that were associated with potential indicators of male quality had also been found previously to be good predictors of female social mate choice. In particular, longer songs with longer elements, performed at lower frequency and with greater consistency, were associated with both female mate choice and potential indicators of quality. Thus, female prairie warblers may assess potential mates with the help of a set of song characteristics that collectively reveal an array of attributes that together indicate overall male quality. C1 [Byers, Bruce E.] Univ Massachusetts Amherst, Dept Biol, Amherst, MA USA. [Akresh, Michael E.] Univ Massachusetts Amherst, Dept Environm Conservat, Amherst, MA USA. [King, David I.] Univ Massachusetts Amherst, US Forest Serv, Northern Res Stn, Amherst, MA USA. RP Byers, BE (reprint author), Univ Massachusetts Amherst, Dept Biol, Morrill Sci Ctr 221, Amherst, MA 01003 USA. EM bbyers@bio.umass.edu FU Nuttall Ornithological Club Blake-Nuttall Fund; U.S. Forest Service, Northern Research Station FX We thank Victoria Phu, Rachel Xue, Ben Lagasse, and Charles Ylijoki for assistance with song analysis, and Brett Bailey, Kayla Baker, Alex Bielaski, Carly Chandler, Jen Glagowski, Brodie Kramer, Tyler Maikath, Nora Papian, Kate Pelletier, Deysha Rivera, Sam Roberts, Josie Ryan-Small, Jen Smetzer, Matt Smith, Greta Turschak, Mattie VandenBoom, Jesse Wampler, Doug Weidemann, and Nathaniel Young for their excellent work in the field. Two anonymous referees provided thoughtful, helpful comments. This work was supported by the Nuttall Ornithological Club Blake-Nuttall Fund and the U.S. Forest Service, Northern Research Station. NR 56 TC 0 Z9 0 U1 16 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-1613 EI 1439-0310 J9 ETHOLOGY JI Ethology PD AUG PY 2016 VL 122 IS 8 BP 660 EP 670 DI 10.1111/eth.12513 PG 11 WC Psychology, Biological; Behavioral Sciences; Zoology SC Psychology; Behavioral Sciences; Zoology GA DR0RP UT WOS:000379614900005 ER PT J AU Mascarin, GM Guarin-Molina, JH Arthurs, SP Humber, RA Moral, RD Demetrio, CGB Delalibera, I AF Mascarin, Gabriel Moura Guarin-Molina, Juan Humberto Arthurs, Steven Paul Humber, Richard Alan Moral, Rafael de Andrade Borges Demetrio, Clarice Garcia Delalibera, Italo, Jr. TI Seasonal prevalence of the insect pathogenic fungus Colletotrichum nymphaeae in Brazilian citrus groves under different chemical pesticide regimes SO FUNGAL ECOLOGY LA English DT Article DE Density-dependence; Natural epizootics; Entomopathogenic fungi; Praelongorthezia praelonga; Climatic factors; Conservational biocontrol ID BIOLOGICAL-CONTROL; ENTOMOPATHOGENS; ACUTATUM; ANTHRACNOSE; EVOLUTION; INFECTION; ECOLOGY; TOMATO AB We report an endemic entomopathogenic fungus, known in Brazil as the 'salmao' fungus and identified here as Colletotrichum nymphaeae (Sordariomycetes: Glomerellales), infecting populations of citrus orthezia scale, Praelongorthezia praelonga. The seasonal prevalence of this pathogen in P. praelonga populations was investigated in three commercial citrus groves maintained under different pesticide regimes. Two citrus groves included inundative releases of another insect pathogenic fungus, Lecanicillium longisporum. Natural epizootics were consistently observed, with up to 84% infection rates being recorded during the warm rainy season. Temporal progression of C. nymphaeae-induced disease varied among the three pesticide regimes. Low infection levels from C. nymphaeae were associated with intensive application of broad spectrum pesticides. However, the prevalence of C. nymphaeae followed a density -dependent pattern with insect host abundance, irrespective of the pesticide regime. High proportions of Lecanicillium-infected insects were observed following infection peaks of C. nymphaeae and both fungi together contributed to 95% overall mortality of citrus orthezia during the wet season. Hence, the combined effect of both fungi considerably improves the biological control of citrus orthezia. We also surmise that the host abundance, environmental conditions, and application frequency of chemical pesticides in citrus groves exert a great influence in the seasonal prevalence of C. nymphaeae-induced disease. Altogether, these results suggest that C. nymphaeae is an important pathogen of P. praelonga and indicate that frequent use of synthetic pesticides may delay or reduce fungal epizootics. (C) 2016 Elsevier Ltd and British Mycological Society. All rights reserved. C1 [Mascarin, Gabriel Moura] Embrapa Arroz & Feijao, Rodovia GO 462,Km 12,CP 179, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Guarin-Molina, Juan Humberto] Corp Colombiana Invest Agr CORPOICA, Ctr Invest La Selva, Rionegro 100, Antioquia, Colombia. [Arthurs, Steven Paul] Univ Florida IFAS, Mid Florida Res & Educ Ctr, Apopka, FL 32703 USA. [Humber, Richard Alan] ARS, USDA, Emerging Pests & Pathogens Res, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. [Moral, Rafael de Andrade; Borges Demetrio, Clarice Garcia] Univ Sao Paulo, Dept Exact Sci, Av Padua Dias 11,CP 9, BR-13418900 Piracicaba, SP, Brazil. [Delalibera, Italo, Jr.] Univ Sao Paulo, Dept Entomol & Acarol, Av Padua Dias 11,CP 9, BR-13418900 Piracicaba, SP, Brazil. RP Mascarin, GM (reprint author), Embrapa Arroz & Feijao, Rodovia GO 462,Km 12,CP 179, BR-75375000 Santo Antonio De Goias, Go, Brazil. EM gabriel.mascarin@embrapa.br; jguarin@corpoica.org.co; spa@ufl.edu; richard.humber@usda.gov; rafael.moral@usp.br; clarice.demetrio@usp.br; delalibera@usp.br RI Moral, Rafael/M-3184-2016; Demetrio, Clarice/I-5263-2012; Mascarin, Gabriel/E-4646-2015; OI Moral, Rafael/0000-0002-0875-3563; Demetrio, Clarice/0000-0002-3609-178X; Mascarin, Gabriel/0000-0003-3517-6826; Delalibera Jr., Italo/0000-0001-9770-9216 FU Cambuhy Agricola Ltd.; CORPOICA (Corporacion Colombiana de Investigacion Agropecuaria) FX Our sincere gratitude to Cambuhy Agricola Ltd. through Dr. Fernando Amado Tersi and Paulo Sperandio for providing support for field studies. The second author was granted with a scholarship from CORPOICA (Corporacion Colombiana de Investigacion Agropecuaria). We are also grateful to anonymous reviewers for their insightful suggestions which improved this manuscript. NR 38 TC 0 Z9 0 U1 3 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1754-5048 EI 1878-0083 J9 FUNGAL ECOL JI Fungal Ecol. PD AUG PY 2016 VL 22 BP 43 EP 51 DI 10.1016/j.funeco.2016.04.005 PG 9 WC Ecology; Mycology SC Environmental Sciences & Ecology; Mycology GA DR0XL UT WOS:000379630100007 ER PT J AU Rogan, J Wright, TM Cardille, J Pearsall, H Ogneva-Himmelberger, Y Riemann, R Riitters, K Partington, K AF Rogan, J. Wright, T. M. Cardille, J. Pearsall, H. Ogneva-Himmelberger, Y. Riemann, R. Riitters, K. Partington, K. TI Forest fragmentation in Massachusetts, USA: a town-level assessment using Morphological spatial pattern analysis and affinity propagation SO GISCIENCE & REMOTE SENSING LA English DT Article DE forest fragmentation; morphological spatial pattern analysis; affinity propagation; Massachusetts ID HABITAT FRAGMENTATION; LANDSCAPES; CANADA AB Forest fragmentation has been studied extensively with respect to biodiversity loss, disruption of ecosystem services, and edge effects although the relationship between forest fragmentation and human activities is still not well understood. We classified the pattern of forests in Massachusetts using fragmentation indicators to address these objectives: 1) characterize the spatial pattern of forest fragmentation in Massachusetts towns using Morphological Spatial Pattern Analysis (MSPA); and (2) identify regional trends using archetypal towns in relation to town history, geography and socioeconomic characteristics. Six fragmentation indicators were calculated using MSPA for each town to represent patterns and processes of fragmentation. We then used these indicators and the proportion of forested land to group towns across Massachusetts with similar patterns of fragmentation. Six representative towns typify different types of forest fragmentation, and illustrate the commonalities and differences between different fragmentation types. The objective selection of representative towns suggests that they might be used as the target of future studies, both in retrospective studies that seek to explain current patterns and in analyses that predict future fragmentation trends. C1 [Rogan, J.; Wright, T. M.] Clark Univ, Clark Grad Sch Geog, Worcester, MA 01610 USA. [Cardille, J.; Partington, K.] Univ Montreal, Dept Geog, Montreal, PQ, Canada. [Cardille, J.] McGill Univ, Dept Nat Resource Sci, Ste Anne De Bellevue, PQ, Canada. [Cardille, J.] McGill Univ, McGill Sch Environm, Ste Anne De Bellevue, PQ, Canada. [Pearsall, H.] Temple Univ, Geog & Urban Studies Dept, Philadelphia, PA 19122 USA. [Ogneva-Himmelberger, Y.] Clark Univ, Dept Int Dev Community & Environm, Worcester, MA 01610 USA. [Riemann, R.] US Forest Serv, No Res Stn, Troy, NY 12180 USA. [Riitters, K.] US Forest Serv, Res Triangle Pk, NC USA. RP Rogan, J (reprint author), Clark Univ, Clark Grad Sch Geog, Worcester, MA 01610 USA. EM jrogan@clarku.edu FU National Science Foundation (NSF) [SES-0849985]; Clark University O'Connor '78 Endowment FX This material is based upon work supported by the National Science Foundation (NSF) [grant number SES-0849985] (REU Site); the Clark University O'Connor '78 Endowment. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the funders. NR 28 TC 2 Z9 2 U1 14 U2 17 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND SN 1548-1603 EI 1943-7226 J9 GISCI REMOTE SENS JI GISci. Remote Sens. PD AUG PY 2016 VL 53 IS 4 BP 506 EP 519 DI 10.1080/15481603.2016.1141448 PG 14 WC Geography, Physical; Remote Sensing SC Physical Geography; Remote Sensing GA DR0NR UT WOS:000379604700005 ER PT J AU Fu, T Hull, JJ Yang, T Wang, G AF Fu, T. Hull, J. J. Yang, T. Wang, G. TI Identification and functional characterization of four transient receptor potential ankyrin 1 variants in Apolygus lucorum (Meyer-Dur) SO INSECT MOLECULAR BIOLOGY LA English DT Article DE TRPA1; thermosensation; mirid; Apolygus lucorum; chemosensation ID MOSQUITO ANOPHELES-GAMBIAE; TRP CHANNELS; DROSOPHILA-MELANOGASTER; ION CHANNELS; COVALENT MODIFICATION; MULTIPLE CROPS; BT COTTON; NEURONS; CHINA; TEMPERATURES AB As signal integrators that respond to various physical and chemical stimuli, transient receptor potential (TRP) channels fulfil critical functional roles in the sensory systems of both vertebrate and invertebrate organisms. Here, four variants of TRP ankyrin 1 (TRPA1) were identified and cloned from the green plant bug, Apolygus lucorum. Spatiotemporal expression profiling across development and in different adult tissues revealed that the highest relative-transcript levels occurred in first-instar nymphs and antennae, respectively. In Xenopus laevis-based functional assays, Apo. lucorum TRPA1-A (AlucTRPA1-A), AlucTRPA1-B and AlucTRPA1-C were activated by increasing the temperature from 20 to 40 degrees C with no significant desensitization observed after repeated temperature stimuli. The activation temperature of AlucTRPA1-A and AlucTRPA1-B was <25 degrees C, whereas the activation temperature of AlucTRPA1-C was between 25 and 30 degrees C. Amongst the variants, only AlucTRPA1-A and AlucTRPA1-C were directly activated by high concentrations of allyl isothiocyanate, cinnamaldehyde and citronellal. Taken together, these results suggest that AlucTRPA1 variants may function in vivo as both thermal and chemical sensors, with the four variants potentially mediating different physiological functions. This study not only enriches our understanding of TRPA1 function in Hemiptera (Miridae), but also offers a foundation for developing new pest control strategies. C1 [Fu, T.; Yang, T.; Wang, G.] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Hull, J. J.] ARS, USDA, Arid Land Agr Ctr, Maricopa, AZ USA. RP Wang, G (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM grwang@ippcaas.cn FU National Genetically Modified Organisms Breeding Major Project [2012ZX08009001]; National Natural Science Foundation of China [31321004, 31471833] FX This work was supported by the National Genetically Modified Organisms Breeding Major Project (2012ZX08009001) and National Natural Science Foundation of China (31321004 and 31471833). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 56 TC 1 Z9 1 U1 6 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD AUG PY 2016 VL 25 IS 4 BP 370 EP 384 DI 10.1111/imb.12231 PG 15 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA DR0QX UT WOS:000379613100004 PM 27038267 ER PT J AU Schetelig, MF Targovska, A Meza, JS Bourtzis, K Handler, AM AF Schetelig, M. F. Targovska, A. Meza, J. S. Bourtzis, K. Handler, A. M. TI Tetracycline-suppressible female lethality and sterility in the Mexican fruit fly, Anastrepha ludens SO INSECT MOLECULAR BIOLOGY LA English DT Article DE female-specific lethality; apoptosis; cell death; sterile insect technique; insect pest management ID CAPITATA DIPTERA TEPHRITIDAE; INSECT TECHNIQUE; EMBRYONIC LETHALITY; GENE-EXPRESSION; SEXING SYSTEM; SUSPENSA; TRANSFORMATION; TRANSCRIPTION; VECTOR; PCR AB The sterile insect technique (SIT) involves the mass release of sterile males to suppress insect pest populations. SIT has been improved for larval pests by the development of strains for female-specific tetracycline-suppressible (Tet-off) embryonic lethal systems for male-only populations. Here we describe the extension of this approach to the Mexican fruit fly, Anastrepha ludens, using a Tet-off driver construct with the Tet-transactivator (tTA) under embryo-specific Anastrepha suspensa serendipity alpha (As-srya) promoter regulation. In the absence of tetracycline, tTA acts upon a Tet-response element linked to the pro-apoptotic cell death gene lethal effector, head involuation defective (hid), from A. ludens (Alhid Ala2) that contains a sex-specific intron splicing cassette, resulting in female-specific expression of the lethal effector. Parental adults double-homozygous for the driver/effector vectors were expected to yield male-only progeny when reared on Tet-free diet, but a complete lack of oviposited eggs resulted for each of the three strains tested. Ovary dissection revealed nonvitellogenic oocytes in all strains that was reversible by feeding females tetracycline for 5 days after eclosion, resulting in male-only adults in one strain. Presumably the sry-alpha promoter exhibits prezygotic maternal expression as well as zygotic embryonic expression in A. ludens, resulting in a Tet-off sterility effect in addition to female-specific lethality. C1 [Schetelig, M. F.] Univ Giessen, Inst Insect Biotechnol, D-35394 Giessen, Germany. [Schetelig, M. F.; Handler, A. M.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Targovska, A.; Bourtzis, K.] Joint FAO IAEA Programme Nucl Techn Food & Agr, Insect Pest Control Lab, Vienna, Austria. [Meza, J. S.] SAGARPA IICA, Programa Moscafrut, Metapa De Dominguez, Chiapas, Mexico. RP Schetelig, MF (reprint author), Univ Giessen, Inst Insect Biotechnol, D-35394 Giessen, Germany. EM marc.schetelig@uni-giessen.de RI Meza Hernandez, Jose Salvador/B-6203-2015 FU United States Department of Agriculture-National Institute of Food and Agriculture-Biotechnology Risk Assessment Grants programme [2011-39211-30769]; Emmy Noether Program of the German Research Foundation [SCHE 1833/1-1]; Landes-Offensive zur Entwicklung Wissenschaftlich-okonomischer Exzellenz Center for Insect Biotechnology & Bioresources of the Hessen State Ministry of Higher Education, Research and the Arts FX We thank Dr Jorge Hendrichs and Dr Walther Enkerlin for helpful comments and additions to the manuscript in respect to the mexfly SIT and its economic impact as well as Tanja Rehling and Derya Arslan for qPCR acquisition and analysis. This project benefitted from discussions at meetings of the Food and Agriculture Organization of the United Nations/International Atomic Energy Agency funded Co-ordinated Research Projects: 'The Use of Molecular Tools to Improve the Effectiveness of SIT' and 'Comparing Rearing Efficiency and Competitiveness of Sterile Male Strains Produced by Genetic, Transgenic or Symbiont-Based Technologies'. Funding is gratefully acknowledged from the United States Department of Agriculture-National Institute of Food and Agriculture-Biotechnology Risk Assessment Grants programme, award #2011-39211-30769 (A.M.H.), the Emmy Noether Program SCHE 1833/1-1 of the German Research Foundation (M.F.S.) and the Landes-Offensive zur Entwicklung Wissenschaftlich-okonomischer Exzellenz Center for Insect Biotechnology & Bioresources of the Hessen State Ministry of Higher Education, Research and the Arts (M.F.S.). NR 23 TC 0 Z9 0 U1 9 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1075 EI 1365-2583 J9 INSECT MOL BIOL JI Insect Mol. Biol. PD AUG PY 2016 VL 25 IS 4 BP 500 EP 508 DI 10.1111/imb.12238 PG 9 WC Biochemistry & Molecular Biology; Entomology SC Biochemistry & Molecular Biology; Entomology GA DR0QX UT WOS:000379613100014 PM 27135433 ER PT J AU Machtinger, ET Leppla, NC Hogsette, JA AF Machtinger, E. T. Leppla, N. C. Hogsette, J. A. TI House and Stable Fly Seasonal Abundance, Larval Development Substrates, and Natural Parasitism on Small Equine Farms in Florida SO NEOTROPICAL ENTOMOLOGY LA English DT Article DE Biological control; Muscidifurax; Pteromalidae; Spalangia ID SPALANGIA-CAMERONI HYMENOPTERA; STOMOXYS-CALCITRANS DIPTERA; FLIES DIPTERA; HOST LOCATION; MUSCIDAE; PTEROMALIDAE; POPULATIONS; AGE; TEMPERATURE; FACILITIES AB House flies, Musca domestica Linnaeus, and stable flies, Stomoxys calcitrans (L.) (Diptera: Muscidae), are common pests on horse farms. The successful use of pupal parasitoids for management of these pests requires knowledge of seasonal fluctuations and biology of the flies as well as natural parasitism levels. However, these dynamics have not been investigated on small equine farms. A 1-year field study began in July 2010, in north central Florida, to determine adult fly population levels and breeding areas on four small equine farms. Weekly surveillance showed that pest flies were present year-round, though there were differences in adult population levels among farms and seasons. Fly development was not confirmed on two of the four small farms, suggesting that subtle differences in husbandry may adversely affect the development of immature flies. In six substrates previously identified as the most common among the farms, stable fly puparia were found overwhelmingly in hay mixed with equine manure and house fly puparia were found in fresh pine shavings mixed with equine manure. Natural parasitism was minimal as expected, but greatest numbers of natural parasitoids collected were of the genus Spalangia. Differences in adult and immature fly numbers recovered emphasizes the need for farm owners to confirm on-site fly development prior to purchase and release of biological control agents. Additionally, due to the low natural parasitism levels and domination of parasitism by Spalangia cameroni, augmentative releases using this species may be the most effective. C1 [Machtinger, E. T.; Leppla, N. C.] Univ Florida, Dept Entomol & Nematol, Charles Steinmetz Hall,1881 Nat Area Dr, Gainesville, FL 32611 USA. [Hogsette, J. A.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Machtinger, ET (reprint author), Univ Florida, Dept Entomol & Nematol, Charles Steinmetz Hall,1881 Nat Area Dr, Gainesville, FL 32611 USA. EM irishtangerine@ufl.edu FU USDA; NIFA; Extension Integrated Pest Management Coordination and Support Program (EIPM-CS) FX We thank Dr. Chris Geden for his review of this manuscript. This work was supported in part by funding from the USDA, NIFA, Extension Integrated Pest Management Coordination and Support Program (EIPM-CS). NR 31 TC 0 Z9 0 U1 8 U2 15 PU ENTOMOLOGICAL SOC BRASIL PI LONDRINA, PA CAIXA POSTAL 481, 86001-970 LONDRINA,, PR, BRAZIL SN 1519-566X EI 1678-8052 J9 NEOTROP ENTOMOL JI Neotrop. Entomol. PD AUG PY 2016 VL 45 IS 4 BP 433 EP 440 DI 10.1007/s13744-016-0376-z PG 8 WC Entomology SC Entomology GA DR2BM UT WOS:000379710000014 PM 26902468 ER PT J AU Knight, AL Basoalto, E Yee, W Hilton, R Kurtzman, CP AF Knight, Alan L. Basoalto, Esteban Yee, Wee Hilton, Rick Kurtzman, Cletus P. TI Adding yeasts with sugar to increase the number of effective insecticide classes to manage Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in cherry SO PEST MANAGEMENT SCIENCE LA English DT Article DE spotted-wing drosophila; yeast; sugar; pest management ID SPOTTED-WING DROSOPHILA; UNITED-STATES; PESTICIDE RESISTANCE; PEST-MANAGEMENT; INVASIVE PEST; SWEET CHERRY; BERRY CROPS; FRUITS; MELANOGASTER; INFESTATION AB BACKGROUNDDrosophila suzukii is a major pest of cherry in the western United States. We evaluated whether the addition of sugary baits could improve the efficacy of two classes of insecticides not considered to be sufficiently effective for this pest, diamides and spinosyns, in laboratory and field trials in cherry. RESULTSAdding cane sugar alone or in combination with the yeasts Saccharomyces cerevisiae or Aureobasidium pullulans significantly improved insecticide efficacy. However, the significance of adding yeasts to the sugar plus insecticide on fly mortality varied with respect to both the insecticide and yeast species. The addition of S. cerevisiae to sugar also did not significantly reduce egg densities in fruit compared with sugar alone. The addition of a yeast plus sugar significantly reduced egg densities in three field trials with cyantraniliprole and in two out of three trials with spinosad. CONCLUSIONThe addition of cane sugar with or without yeast can improve the effectiveness of diamide and spinosyn insecticides for D. suzukii in cherry. Inclusion of these two insecticides in D. suzukii management programs may alleviate the strong selection pressure currently being imposed on a few mode-of-action insecticide classes used by growers to maintain fly suppression over long continuous harvest periods of mixed cultivars. Published 2015. This article is a U.S. Government work and is in the public domain in the USA. C1 [Knight, Alan L.; Yee, Wee] ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. [Basoalto, Esteban] Univ Austral Chile, Inst Prod & Sanidad Vegetal, Fac Ciencias Agr, Valdivia, Chile. [Hilton, Rick] Oregon State Univ, Southern Oregon Res & Extens Ctr, Medford, OR USA. [Kurtzman, Cletus P.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, USDA, Peoria, IL USA. RP Knight, AL (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov NR 52 TC 1 Z9 1 U1 23 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD AUG PY 2016 VL 72 IS 8 BP 1482 EP 1490 DI 10.1002/ps.4171 PG 9 WC Agronomy; Entomology SC Agriculture; Entomology GA DR0MN UT WOS:000379601700005 PM 26454150 ER PT J AU Accinelli, C Abbas, HK Vicari, A Shier, WT AF Accinelli, Cesare Abbas, Hamed K. Vicari, Alberto Shier, W. Thomas TI Leaf application of a sprayable bioplastic-based formulation of biocontrol Aspergillus flavus strains for reduction of aflatoxins in corn SO PEST MANAGEMENT SCIENCE LA English DT Article DE aflatoxins; Aspergillus flavus; sprayable bioplastic; biopesticides; biological control; Zea mays; maize ID UNITED-STATES; SECTION FLAVI; CONTAMINATION; POPULATIONS; ECOLOGY; PELLETS; CROPS; SOIL AB BACKGROUNDApplying non-aflatoxin-producing Aspergillus flavus isolates to the soil has been shown to be effective in reducing aflatoxin levels in harvested crops, including peanuts, cotton and corn. The aim of this study was to evaluate the possibility of controlling aflatoxin contamination using a novel sprayable formulation consisting of a partially gelatinized starch-based bioplastic dispersion embedded with spores of biocontrol A. flavus strains, which is applied to the leaf surfaces of corn plants. RESULTSThe formulation was shown to be adherent, resulting in colonization of leaf surfaces with the biocontrol strain of A. flavus, and to reduce aflatoxin contamination of harvested kernels by up to 80% in Northern Italy and by up to 89% in the Mississippi Delta. The percentage of aflatoxin-producing isolates in the soil reservoir under leaf-treated corn was not significantly changed, even when the soil was amended with additional A. flavus as a model of changes to the soil reservoir that occur in no-till agriculture. CONCLUSIONSThis study indicated that it is not necessary to treat the soil reservoir in order to achieve effective biocontrol of aflatoxin contamination in kernel corn. Spraying this novel bioplastic-based formulation to leaves can be an effective alternative in the biocontrol of A. flavus in corn. (c) 2015 Society of Chemical Industry C1 [Accinelli, Cesare; Vicari, Alberto] Alma Mater Studiorum Univ Bologna, Dept Agr Sci, Viale Fanin 44, I-40127 Bologna, Italy. [Abbas, Hamed K.] ARS, USDA, Biol Control Pests Res Unit, Stoneville, MS USA. [Shier, W. Thomas] Univ Minnesota, Coll Pharm, Dept Med Chem, Minneapolis, MN 55455 USA. RP Accinelli, C (reprint author), Alma Mater Studiorum Univ Bologna, Dept Agr Sci, Viale Fanin 44, I-40127 Bologna, Italy. EM cesare.accinelli@unibo.it NR 27 TC 1 Z9 1 U1 8 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD AUG PY 2016 VL 72 IS 8 BP 1521 EP 1528 DI 10.1002/ps.4180 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA DR0MN UT WOS:000379601700010 PM 26518170 ER PT J AU Schreiber, KJ Baudin, M Hassan, JA Lewis, JD AF Schreiber, Karl J. Baudin, Mael Hassan, Jana A. Lewis, Jennifer D. TI Die another day: Molecular mechanisms of effector-triggered immunity elicited by type III secreted effector proteins SO SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY LA English DT Review DE Effector-triggered-immunity; Type III secreted effector protein; Plant pathogenic bacteria; NOD-like receptor ID PSEUDOMONAS-SYRINGAE EFFECTOR; DISEASE RESISTANCE GENE; BACTERIAL SPECK DISEASE; NUCLEOTIDE-BINDING SITE; PROGRAMMED CELL-DEATH; PLANT PLASMA-MEMBRANE; E3 UBIQUITIN LIGASE; RICH REPEAT DOMAIN; NB-LRR PROTEIN; ARABIDOPSIS-THALIANA AB Bacterial pathogens inject type III secreted effector (T3SE) proteins into their hosts where they display dual roles depending on the host genotype. T3SEs promote bacterial virulence in susceptible hosts, and elicit immunity in resistant hosts. T3SEs are typically recognized when they modify a host target that is associated with a NOD-like receptor protein. We focus on the molecular mechanisms of T3SE recognition in plants. Plants guard multiple nodes of the immune signaling pathway, from recognition at the cell surface by receptor-like kinases to nuclear signaling. Some nodes are bacterial virulence targets, while other nodes are decoys that resemble true virulence targets. Published by Elsevier Ltd. C1 [Schreiber, Karl J.; Baudin, Mael; Hassan, Jana A.; Lewis, Jennifer D.] Univ Calif Berkeley, Dept Plant & Microbial Biol, 800 Buchanan St, Albany, CA 94710 USA. [Lewis, Jennifer D.] Univ Calif Berkeley, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. RP Lewis, JD (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, 800 Buchanan St, Albany, CA 94710 USA.; Lewis, JD (reprint author), Univ Calif Berkeley, USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. EM karl.schreiber@berkeley.edu; mael.baudin@berkeley.edu; jana.a.hassan@berkeley.edu; jdlewis@berkeley.edu FU United States Department of Agriculture Agricultural Research Service [5335-21000-040-00D]; Institut National de la Recherche Agronomique (INRA) postdoctoral fellowship FX We apologize to any colleagues whose work we could not discuss due to limited space. Research on plant immunity in the Lewis laboratory was supported by United States Department of Agriculture Agricultural Research Service 5335-21000-040-00D (J.D.L), and an Institut National de la Recherche Agronomique (INRA) postdoctoral fellowship (M.B.). NR 185 TC 1 Z9 1 U1 19 U2 21 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1084-9521 J9 SEMIN CELL DEV BIOL JI Semin. Cell Dev. Biol. PD AUG PY 2016 VL 56 BP 124 EP 133 DI 10.1016/j.semcdb.2016.05.001 PG 10 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DR1YF UT WOS:000379700100015 PM 27166224 ER PT J AU Underwood, W AF Underwood, William TI Contributions of host cellular trafficking and organization to the outcomes of plant-pathogen interactions SO SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY LA English DT Review DE Plant pathogen; Plant disease; Vesicle trafficking; Actin; Haustorium; Papilla ID ACTIN-DEPOLYMERIZING FACTOR; LIGAND-INDUCED ENDOCYTOSIS; RESISTANCE PROTEIN RPW8.2; POWDERY MILDEW FUNGUS; DISEASE-RESISTANCE; PHYTOPHTHORA-INFESTANS; MAGNAPORTHE-ORYZAE; PLASMA-MEMBRANE; NONHOST RESISTANCE; IMMUNE-RESPONSES AB In recent years it has become increasingly apparent that dynamic changes in protein localization, membrane trafficking pathways, and cellular organization play a major role in determining the outcome of interactions between plants and pathogenic microorganisms. Plants have evolved sophisticated perception systems to recognize the presence of potentially pathogenic microorganisms via the detection of non-self or modified-self elicitor molecules, pathogen virulence factors, or the activities of such virulence factors. Dynamic changes to host cellular organization and membrane trafficking pathways play pivotal roles in detection and signaling by plant immune receptors and are vital for the execution of spatially targeted defense responses to thwart invasion by potential pathogens. Conversely, from the perspective of the pathogen, the ability to manipulate plant cellular organization and trafficking processes to establish infection structures and deliver virulence factors is a major determinant of pathogen success. This review summarizes selected topics that illustrate how dynamic changes in host cellular trafficking and organization shape the outcomes of diverse plant-pathogen interactions and addresses ways in which our rapidly expanding knowledge of the cell biological processes that contribute to immunity or infection may influence efforts to improve plant disease resistance. Published by Elsevier Ltd. C1 [Underwood, William] USDA ARS, Sunflower & Plant Biol Res Unit, Northern Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. RP Underwood, W (reprint author), USDA ARS, Sunflower & Plant Biol Res Unit, Northern Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM William.Underwood@ars.usda.gov FU USDA Agricultural Research Service FX The author wishes to apologize to colleagues whose work was not discussed in this article due to space limitations. This work was supported by funding from the USDA Agricultural Research Service. USDA is an equal opportunity provider and employer. NR 143 TC 0 Z9 0 U1 17 U2 30 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1084-9521 J9 SEMIN CELL DEV BIOL JI Semin. Cell Dev. Biol. PD AUG PY 2016 VL 56 BP 163 EP 173 DI 10.1016/j.semcdb.2016.05.016 PG 11 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA DR1YF UT WOS:000379700100018 PM 27216829 ER PT J AU Garcia, KS Power, TG Fisher, JO O'Connor, TM Hughes, SO AF Garcia, Karina Silva Power, Thomas G. Fisher, Jennifer Orlet O'Connor, Teresia M. Hughes, Sheryl O. TI Latina mothers' influences on child appetite regulation SO APPETITE LA English DT Article DE Eating in the absence of hunger; Children's Eating Behavior Questionnaire; Appetite regulation; Head Start; Feeding behaviors; Preschoolers ID MATERNAL FEEDING PRACTICES; EATING BEHAVIOR QUESTIONNAIRE; CALORIC COMPENSATION; PRESCHOOL-CHILDREN; HISPANIC CHILDREN; SELF-REGULATION; OBESITY; ABSENCE; HUNGER; WEIGHT AB Parents influence child weight through interactions that shape the development of child eating behaviors. In this study we examined the association between maternal autonomy promoting serving practices and child appetite regulation. We predicted that maternal autonomy promoting serving practices would be positively associated with child appetite regulation. Participants were low-income Latino children a group at high risk for the development of childhood obesity. A total of 186 low-income Latina mothers and their 4-5 year old children came to a laboratory on two separate days. On the first day, mothers and children chose foods for a meal from a buffet and were audio/videotaped so that maternal autonomy promoting serving practices could be later coded. On the second day, children completed the Eating in the Absence of Hunger (EAH) task to measure child appetite regulation. Mothers also completed the Child Eating Behavior Questionnaire (CEBQ) to measure other aspects of child appetite regulation (food responsiveness, satiety responsiveness, and emotional overeating). Maternal autonomy promotion during serving was assessed using seven separate measures of child and maternal behavior. Principal components analyses of these serving measures yielded three components: allows child choice, child serves food, and mother does not restrict. Consistent with hypotheses, maternal autonomy promoting serving practices (i.e., allows child choice and does not restrict) were negatively associated with maternal reports of child food responsiveness and emotional overeating (CEBQ). The results for the EAH task were more complex mothers who were autonomy promoting in their serving practices had children who ate the most in the absence of hunger, but this linear effect was moderated somewhat by a quadratic effect, with moderate levels of autonomy promotion during serving associated with the greatest child EAH. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Garcia, Karina Silva; Power, Thomas G.] Washington State Univ, Dept Human Dev, POB 644852, Pullman, WA 99164 USA. [Fisher, Jennifer Orlet] Temple Univ, Sch Med, Ctr Obes Res & Educ, 3223 N Broad St Suite 175, Philadelphia, PA 19140 USA. [O'Connor, Teresia M.; Hughes, Sheryl O.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. RP Hughes, SO (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St, Houston, TX 77030 USA. EM kari.silva@wsu.edu; tompower@wsu.edu; jofisher@temple.edu; teresiao@bcm.edu; shughes@bcm.edu FU National Institute of Child Health and Human Development [R01 HD062567]; USDA/ARS [6250-51000] FX This research was supported by funds from the National Institute of Child Health and Human Development grant R01 HD062567 to Sheryl O. Hughes, Baylor College of Medicine, Houston, Texas. This work is also a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX) funded in part by the USDA/ARS (Cooperative Agreement 6250-51000). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. Special thanks to Dr. Raymond Herrera, Mary Crowell and Nicole Cristobal for their support and guidance during this project, and to Leslie Fernanda-Diaz Velasco and Maile Ann Scarpio for assistance in coding the videotapes. NR 55 TC 1 Z9 1 U1 17 U2 23 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD AUG 1 PY 2016 VL 103 BP 200 EP 207 DI 10.1016/j.appet.2016.04.013 PG 8 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA DP7GF UT WOS:000378666500025 ER PT J AU O'Connor, TM Pham, T Watts, AW Tu, AW Hughes, SO Beauchamp, MR Baranowski, T Masse, LC AF O'Connor, Teresia M. Pham, Truc Watts, Allison W. Tu, Andrew W. Hughes, Sheryl O. Beauchamp, Mark R. Baranowski, Tom Masse, Louise C. TI Development of an item bank for food parenting practices based on published instruments and reports from Canadian and US parents SO APPETITE LA English DT Article DE Parenting practices; Food; Nutrition; Child; Item bank; Systematic review; Parents ID MATERNAL FEEDING PRACTICES; BALANCE-RELATED BEHAVIORS; BODY-MASS INDEX; EATING BEHAVIOR; CHILDREN; WEIGHT; OVERWEIGHT; OBESITY; INCOME; DIET AB Research to understand how parents influence their children's dietary intake and eating behaviors has expanded in the past decades and a growing number of instruments are available to assess food parenting practices. Unfortunately, there is no consensus on how constructs should be defined or operationalized, making comparison of results across studies difficult. The aim of this study was to develop a food parenting practice item bank with items from published scales and supplement with parenting practices that parents report using. Items from published scales were identified from two published systematic reviews along with an additional systematic review conducted for this study. Parents (n = 135) with children 5-12 years old from the US and Canada, stratified to represent the demographic distribution of each country, were recruited to participate in an online semi-qualitative survey on food parenting. Published items and parent responses were coded using the same framework to reduce the number of items into representative concepts using a binning and winnowing process. The literature contributed 1392 items and parents contributed 1985 items, which were reduced to 262 different food parenting concepts (26% exclusive from literature, 12% exclusive from parents, and 62% represented in both). Food parenting practices related to 'Structure of Food Environment' and 'Behavioral and Educational' were emphasized more by parent responses, while practices related to 'Consistency of Feeding Environment' and 'Emotional Regulation' were more represented among published items. The resulting food parenting item bank should next be calibrated with item response modeling for scientists to use in the future. (C) 2016 Elsevier Ltd. All rights reserved. C1 [O'Connor, Teresia M.; Pham, Truc; Hughes, Sheryl O.; Baranowski, Tom] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Watts, Allison W.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN USA. [Tu, Andrew W.; Masse, Louise C.] Univ British Columbia, Sch Populat & Publ Hlth, Child & Family Res Inst, Vancouver, BC V5Z 1M9, Canada. [Beauchamp, Mark R.] Univ British Columbia, Sch Kinesiol, Vancouver, BC V5Z 1M9, Canada. RP O'Connor, TM (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM teresiao@bcm.edu FU Canadian Institute of Health Research (CIHR) [20R06534]; Chid & Family Research Institute (CFRI); CIHR; CFRI; Michael Smith Foundation for Health Research; USDA/ARS [6250-51000] FX The project described was supported by award number 20R06534 from the Canadian Institute of Health Research (CIHR) awarded to LCM; LCM received salary support from the Chid & Family Research Institute (CFRI); AWW received post-doctoral support from CIHR; and AT received post-doctoral support from CFRI and the Michael Smith Foundation for Health Research.; This work is also a publication of the US Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, BCM funded in part by the USDA/ARS (Cooperative Agreement 6250-51000). The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of organizations imply endorsement from the US government. NR 47 TC 0 Z9 0 U1 2 U2 9 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD AUG 1 PY 2016 VL 103 BP 386 EP 395 DI 10.1016/j.appet.2016.04.033 PG 10 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA DP7GF UT WOS:000378666500047 PM 27131416 ER PT J AU Freydier, L Lundgren, JG AF Freydier, Laurene Lundgren, Jonathan G. TI Unintended effects of the herbicides 2,4-D and dicamba on lady beetles SO ECOTOXICOLOGY LA English DT Article DE Coleomegilla maculata; GM crop; Herbicide tolerant crop; Pesticide; Risk assessment; Sublethal effects ID TRANSGENIC CORN POLLEN; COLEOMEGILLA-MACULATA; WEED MANAGEMENT; APIS-MELLIFERA; BODY-SIZE; GLYPHOSATE; PESTICIDES; TOXICITY; COCCINELLIDAE; FORMULATION AB Weed resistance to glyphosate and development of new GM crops tolerant to 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba is expected to lead to increased use of these herbicides in cropland. The lady beetle, Coleomegilla maculata is an important beneficial insect in cropland that is commonly used as an indicator species in safety evaluations of pesticides. Here, we examined the lethal and non-lethal effects of 2,4-D and dicamba active ingredients and commercial formulations to this lady beetle species, and tested for synergistic effects of the herbicides. Second instars of lady beetles were exposed to an experimental treatment, and their mortality, development, weight, sex ratio, fecundity, and mobility was evaluated. Using similar methods, a dose-response study was conducted on 2,4-D with and without dicamba. The commercial formulation of 2,4-D was highly lethal to lady beetle larvae; the LC90 of this herbicide was 13 % of the label rate. In this case, the "inactive" ingredients were a key driver of the toxicity. Dicamba active ingredient significantly increased lady beetle mortality and reduced their body weight. The commercial formulations of both herbicides reduced the proportion of males in the lady beetle population. The herbicides when used together did not act synergistically in their toxicity toward lady beetles versus when the chemistries were used independently. Our work shows that herbicide formulations can cause both lethal and sublethal effects on non-target, beneficial insects, and these effects are sometimes driven by the "inactive" ingredients. The field-level implications of shifts in weed management practices on insect management programs should receive further attention. C1 [Freydier, Laurene] Agrocampus Ouest, 65 Rue St Brieuc,CS 842015, F-35042 Rennes, France. [Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Lundgren, Jonathan G.] Ecdysis Fdn, 46958 188th St, Estelline, SD 57234 USA. RP Lundgren, JG (reprint author), USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA.; Lundgren, JG (reprint author), Ecdysis Fdn, 46958 188th St, Estelline, SD 57234 USA. EM jgl.entomology@gmail.com NR 54 TC 0 Z9 0 U1 29 U2 35 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0963-9292 EI 1573-3017 J9 ECOTOXICOLOGY JI Ecotoxicology PD AUG PY 2016 VL 25 IS 6 BP 1270 EP 1277 DI 10.1007/s10646-016-1680-4 PG 8 WC Ecology; Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA DQ2KQ UT WOS:000379031600019 PM 27282375 ER PT J AU Romolini, M Grove, JM Ventriss, CL Koliba, CJ Krymkowski, DH AF Romolini, Michele Grove, J. Morgan Ventriss, Curtis L. Koliba, Christopher J. Krymkowski, Daniel H. TI Toward an Understanding of Citywide Urban Environmental Governance: An Examination of Stewardship Networks in Baltimore and Seattle SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Urban sustainability; Governance networks; Social network analysis; Urban natural resources management ID NATURAL-RESOURCE MANAGEMENT; SOCIAL NETWORKS; ECOSYSTEM SERVICES; MISSING DATA; CENTRALITY; STOCKHOLM; TIES AB Efforts to create more sustainable cities are evident in the proliferation of sustainability policies in cities worldwide. It has become widely proposed that the success of these urban sustainability initiatives will require city agencies to partner with, and even cede authority to, organizations from other sectors and levels of government. Yet the resulting collaborative networks are often poorly understood, and the study of large whole networks has been a challenge for researchers. We believe that a better understanding of citywide environmental governance networks can inform evaluations of their effectiveness, thus contributing to improved environmental management. Through two citywide surveys in Baltimore and Seattle, we collected data on the attributes of environmental stewardship organizations and their network relationships. We applied missing data treatment approaches and conducted social network and comparative analyses to examine (a) the organizational composition of the network, and (b) how information and knowledge are shared throughout the network. Findings revealed similarities in the number of actors and their distribution across sectors, but considerable variation in the types and locations of environmental stewardship activities, and in the number and distribution of network ties in the networks of each city. We discuss the results and potential implications of network research for urban sustainability governance. C1 [Romolini, Michele] Loyola Marymount Univ, Ctr Urban Resilience, Res Annex 113,1 LMU Dr, Los Angeles, CA 90045 USA. [Grove, J. Morgan] US Forest Serv, Northern Res Stn, USDA, Suite 350,5523 Res Pk Dr, Baltimore, MD 21228 USA. [Ventriss, Curtis L.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, 81 Carrigan Dr, Burlington, VT 05405 USA. [Koliba, Christopher J.] Univ Vermont, Dept Community Dev & Appl Econ, 103D Morrill Hall, Burlington, VT 05405 USA. [Krymkowski, Daniel H.] Univ Vermont, Dept Sociol, 31 South Prospect St, Burlington, VT 05405 USA. RP Romolini, M (reprint author), Loyola Marymount Univ, Ctr Urban Resilience, Res Annex 113,1 LMU Dr, Los Angeles, CA 90045 USA. EM michele.romolini@lmu.edu FU ARRA Project [10-CA-11260489-090]; DC-BC ULTRA-Ex [NSF-DEB-0948947]; [NSF-DEB-0423476] FX This study was part of Dr. Romolini's unpublished doctoral dissertation from the University of Vermont. We are grateful to all of the organizations in Baltimore and Seattle who participated in the survey, and particularly to those who offered valuable input along the way. We acknowledge that this research would not have been possible without material and intellectual support from the USDA Forest Service's Pacific Northwest and Northern Research stations-particularly Dale Blahna and Kathy Wolf for their mentorship and guidance during field seasons in Seattle, and Lindsay Campbell, Dana Fisher, and Erika Svendsen for developing the foundational STEW-MAP research design in New York City and collaborating to refine the survey instrument along with Lynne Westphal and Cherie Fisher in Chicago. Financial support was provided by ARRA Project 10-CA-11260489-090, NSF-DEB-0423476, and DC-BC ULTRA-Ex NSF-DEB-0948947. We also thank Naim Kapucu for a thoughtful review of an earlier version of the paper. Any opinions, findings, and conclusions or recommendations expressed in this manuscript are those of the authors and do not necessarily reflect the views of any of the contributors. NR 77 TC 1 Z9 1 U1 8 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD AUG PY 2016 VL 58 IS 2 BP 254 EP 267 DI 10.1007/s00267-016-0704-4 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ4FK UT WOS:000379159200006 PM 27145945 ER PT J AU Hirabayashi, S Nowak, DJ AF Hirabayashi, Satoshi Nowak, David J. TI Comprehensive national database of tree effects on air quality and human health in the United States SO ENVIRONMENTAL POLLUTION LA English DT Article DE Air quality; Dry deposition; Human health; National database; Forest ID POLLUTION; EXPOSURE; MODEL; HYDROCARBONS; REMOVAL; OZONE; PM2.5; US AB Trees remove air pollutants through dry deposition processes depending upon forest structure, meteorology, and air quality that vary across space and time. Employing nationally available forest, weather, air pollution and human population data for 2010, computer simulations were performed for deciduous and evergreen trees with varying leaf area index for rural and urban areas in every county in the conterminous United States. The results populated a national database of annual air pollutant removal, concentration changes, and reductions in adverse health incidences and costs for NO2, O-3, PM2.5 and SO2. The developed database enabled a first order approximation of air quality and associated human health benefits provided by trees with any forest configurations anywhere in the conterminous United States over time. Comprehensive national database of tree effects on air quality and human health in the United States was developed. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Hirabayashi, Satoshi] SUNY, Davey Tree Expert Co, Davey Inst, 5 Moon Lib, Syracuse, NY 13210 USA. [Nowak, David J.] SUNY, USDA, US Forest Serv, 5 Moon Lib, Syracuse, NY 13210 USA. RP Hirabayashi, S (reprint author), SUNY, Davey Tree Expert Co, Davey Inst, 5 Moon Lib, Syracuse, NY 13210 USA. EM satoshi.hirabayashi@davey.com; dnowak@fe.fed.us OI Hirabayashi, Satoshi/0000-0003-4259-2748 NR 42 TC 2 Z9 2 U1 28 U2 48 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD AUG PY 2016 VL 215 BP 48 EP 57 DI 10.1016/j.envpol.2016.04.068 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1KX UT WOS:000378961000006 PM 27176764 ER PT J AU Oikawa, S Ainsworth, EA AF Oikawa, Shimpei Ainsworth, Elizabeth A. TI Changes in leaf area, nitrogen content and canopy photosynthesis in soybean exposed to an ozone concentration gradient SO ENVIRONMENTAL POLLUTION LA English DT Article DE Canopy photosynthesis model; Light attenuation; Sensitivity analyses; SoyFACE; Tropospheric ozone ID ELEVATED CO2; TROPOSPHERIC OZONE; CARBON GAIN; GLOBAL RADIATION; DIRECT COMPONENT; FAGUS-SYLVATICA; GROWTH; LEAVES; YIELD; PRODUCTIVITY AB Influences of ozone (O-3) on light-saturated rates of photosynthesis in crop leaves have been well documented. To increase our understanding of O-3 effects on individual- or stand level productivity, a mechanistic understanding of factors determining canopy photosynthesis is necessary. We used a canopy model to scale photosynthesis from leaf to canopy, and analyzed the importance of canopy structural and leaf ecophysiological characteristics in determining canopy photosynthesis in soybean stands exposed to 9 concentrations of [O-3] (37-116 ppb; 9-h mean). Light intensity and N content peaked in upper canopy layers, and sharply decreased through the lower canopy. Plant leaf area decreased with increasing [O-3] allowing for greater light intensity to reach lower canopy levels. At the leaf level, light-saturated photosynthesis decreased and dark respiration increased with increasing [O-3]. These data were used to calculate daily net canopy photosynthesis (P-c). P-c decreased with increasing [O-3] with an average decrease of 10% for an increase in [O-3] of 10 ppb, and which was similar to changes in above-ground dry mass production of the stands. Absolute daily net photosynthesis of lower layers was very low and thus the decrease in photosynthesis in the lower canopy caused by elevated [O-3] had only minor significance for total canopy photosynthesis. Sensitivity analyses revealed that the decrease in Pc was associated with changes in leaf ecophysiology but not with decrease in leaf area. The soybean stands were very crowded, the leaves were highly mutually shaded, and sufficient light for positive carbon balance did not penetrate to lower canopy leaves, even under elevated [O-3]. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Oikawa, Shimpei] Ibaraki Univ, Coll Sci, Mito, Ibaraki 3100056, Japan. [Oikawa, Shimpei] Ibaraki Univ, Inst Global Change Adaptat Sci, Mito, Ibaraki 3100056, Japan. [Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL USA. [Ainsworth, Elizabeth A.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. RP Oikawa, S (reprint author), Ibaraki Univ, Coll Sci, Mito, Ibaraki 3100056, Japan. EM shimpei.oikawa.dx@vc.ibaraki.ac.jp FU Japan Society for the Promotion of Science [07J09565, 23770027] FX We thank Carl Bernacchi, Amy Betzelberger, Jessica Chiang, Frank Doleman, John Drake, Sharon Gray, Doug Klier, Bob Koester, Andrew Leakey, Cody Markelz, Charles Mitsdarfer, Don Ort, Kannan Puthuval, Carrie Ramig, David Rosenthal, Reid Strellner and Craig Yendrek for assistance. This work was supported by Grant-in-Aid for JSPS Fellows (07J09565) and Grant-in-Aid for Young Scientists to S. Oikawa (23770027) from the Japan Society for the Promotion of Science. NR 65 TC 0 Z9 0 U1 24 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD AUG PY 2016 VL 215 BP 347 EP 355 DI 10.1016/j.envpol.2016.05.005 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DQ1KX UT WOS:000378961000038 PM 27261884 ER PT J AU Ekinci, FY Baser, GM Ozcan, E Ustundag, OG Korachi, M Sofu, A Blumberg, JB Chen, CYO AF Ekinci, Fatma Y. Baser, Gamze M. Ozcan, Ezgi Ustundag, Ozlem Guclu Korachi, May Sofu, Aytul Blumberg, Jeffrey B. Chen, C. -Y. Oliver TI Characterization of chemical, biological, and antiproliferative properties of fermented black carrot juice, shalgam SO EUROPEAN FOOD RESEARCH AND TECHNOLOGY LA English DT Article DE Fermented black carrot juice; Shalgam; Anthocyanins; Antioxidant capacity; Lactic acid bacteria; Antiproliferative activity ID LACTIC-ACID BACTERIA; TOTAL PHENOLIC CONTENT; CANCER CACO-2 CELLS; ANTIOXIDANT PROPERTIES; GENOME SEQUENCE; LACTOBACILLUS-DELBRUECKII; FUNCTIONAL FOODS; ANTHOCYANINS; BEVERAGES; QUALITY AB Shalgam juice is a dark red-colored and sour fermented beverage produced and consumed in Turkey. The main ingredient of shalgam juice is black carrot, which is rich in anthocyanins. In this study, commercially available shalgam juice was characterized by determining its chemical composition and antioxidant capacity and by identifying its microflora. The predominant acid in the shalgam juice was lactic acid. LC/MS/MS analysis revealed the presence of the anthocyanins cyanidin-3-galactoside, cyanidin-3-glucoside, and cyanidin-3-arabinoside. The total phenolic content (517.21 A mu g GAE/mL) and antioxidant capacity (in A mu mol Trolox equivalents/mL) determined by ABTS (3.42), DPPH (4.44) and FRAP (2.26) assays of the commercial shalgam juice were similar to other common fruit juices. A total of 21 Lactobacillus species and subspecies were identified in shalgam juice using species-specific PCR with the nucleotide sequences of some of the identified lactic acid bacteria. Shalgam juice inhibited the growth of Caco-2 cells lines in a dose-dependent manner and had significantly higher inhibition at 3200 A mu g/mL compared to black carrot (p < 0.05).These results suggest that in addition to the actions of it (poly)phenolic constituents, shalgam juice might have a potent as antioxidant, probiotic and antiproliferative agents. C1 [Ekinci, Fatma Y.; Baser, Gamze M.; Ozcan, Ezgi; Ustundag, Ozlem Guclu] Yeditepe Univ, Dept Food Engn, TR-34755 Istanbul, Turkey. [Korachi, May] Yeditepe Univ, Genet & Bioengn Dept, Istanbul, Turkey. [Sofu, Aytul] Suleyman Demirel Univ, Dept Chem Engn, Isparta, Turkey. [Blumberg, Jeffrey B.; Chen, C. -Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Medford, MA 02155 USA. RP Ekinci, FY (reprint author), Yeditepe Univ, Dept Food Engn, TR-34755 Istanbul, Turkey. EM yekinci@gmail.com NR 61 TC 0 Z9 0 U1 22 U2 29 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1438-2377 EI 1438-2385 J9 EUR FOOD RES TECHNOL JI Eur. Food Res. Technol. PD AUG PY 2016 VL 242 IS 8 BP 1355 EP 1368 DI 10.1007/s00217-016-2639-7 PG 14 WC Food Science & Technology SC Food Science & Technology GA DQ4OU UT WOS:000379184000016 ER PT J AU Bolster, CH Vadas, PA Boykin, D AF Bolster, Carl H. Vadas, Peter A. Boykin, Debbie TI Model parameter uncertainty analysis for an annual field-scale P loss model SO JOURNAL OF HYDROLOGY LA English DT Article DE Annual P Loss Estimator model (APLE); Model uncertainty; Confidence intervals; Prediction intervals; Phosphorus modeling ID LEAST-SQUARES ANALYSIS; WATER-QUALITY; DISSOLVED PHOSPHORUS; STREAMFLOW SIMULATION; DECISION-SUPPORT; SORPTION DATA; RUNOFF; SOIL; SENSITIVITY; FRAMEWORK AB Phosphorous (P) fate and transport models are important tools for developing and evaluating conservation practices aimed at reducing P losses from agricultural fields. Because all models are simplifications of complex systems, there will exist an inherent amount of uncertainty associated with their predictions. It is therefore important that efforts be directed at identifying, quantifying, and communicating the different sources of model uncertainties. In this study, we conducted an uncertainty analysis with the Annual P Loss Estimator (APLE) model. Our analysis included calculating parameter uncertainties and confidence and prediction intervals for five internal regression equations in APLE. We also estimated uncertainties of the model input variables based on values reported in the literature. We then predicted P loss for a suite of fields under different management and climatic conditions while accounting for uncertainties in the model parameters and inputs and compared the relative contributions of these two sources of uncertainty to the overall uncertainty associated with predictions of P loss. Both the overall magnitude of the prediction uncertainties and the relative contributions of the two sources of uncertainty varied depending on management practices and field characteristics. This was due to differences in the number of model input variables and the uncertainties in the regression equations associated with each P loss pathway. Inspection of the uncertainties in the five regression equations brought attention to a previously unrecognized limitation with the equation used to partition surface-applied fertilizer P between leaching and runoff losses. As a result, an alternate equation was identified that provided similar predictions with much less uncertainty. Our results demonstrate how a thorough uncertainty and model residual analysis can be used to identify limitations with a model. Such insight can then be used to guide future data collection and model development and evaluation efforts. Published by Elsevier B.V. C1 [Bolster, Carl H.] ARS, Food Anim Environm Syst Res Unit, USDA, Bowling Green, KY 42101 USA. [Vadas, Peter A.] ARS, Daily Forage Res Ctr, USDA, Madison, WI 53706 USA. [Boykin, Debbie] ARS, USDA, Stoneville, MS 38776 USA. RP Bolster, CH (reprint author), ARS, Food Anim Environm Syst Res Unit, USDA, Bowling Green, KY 42101 USA. EM carl.bolster@ars.usda.gov NR 72 TC 0 Z9 0 U1 7 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG PY 2016 VL 539 BP 27 EP 37 DI 10.1016/j.jhydrol.2016.05.009 PG 11 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DQ1IC UT WOS:000378953700003 ER PT J AU Bunte, K Swingle, KW Turowski, JM Abt, SR Cenderelli, DA AF Bunte, Kristin Swingle, Kurt W. Turowski, Jens M. Abt, Steven R. Cenderelli, Daniel A. TI Measurements of coarse particulate organic matter transport in steep mountain streams and estimates of decadal CPOM exports SO JOURNAL OF HYDROLOGY LA English DT Article DE Rocky Mountains; Mountain streams; Sampling methods and schemes; Transport relations; Hysteresis; Carbon export ID SOUTHWESTERN BRITISH-COLUMBIA; HEADWATER STREAMS; BEDLOAD TRANSPORT; LARGE WOOD; NUTRIENT DYNAMICS; CARBON STORAGE; RIVER LOADS; DEBRIS DAMS; BEAR BROOK; USA AB Coarse particulate organic matter (CPOM) provides a food source for benthic organisms, and the fluvial transport of CPOM is one of the forms in which carbon is exported from a forested basin. However, little is known about transport dynamics of CPOM, its relation to discharge, and its annual exports from mountain streams. Much of this knowledge gap is due to sampling difficulties. In this study, CPOM was sampled over one-month snowmelt high flow seasons in two high-elevation, subalpine, streams in the Rocky Mountains. Bedload traps developed for sampling gravel bedload were found to be suitable samplers for CPOM transport. CPOM transport rates were well related to flow in consecutive samples but showed pronounced hysteresis over the diurnal fluctuations of flow, between consecutive days, and over the rising and falling limbs of the high-flow season. In order to compute annual CPOM load, hysteresis effects require intensive sampling and establishing separate rating curves for all rising and falling limbs. Hysteresis patterns of CPOM transport relations identified in the well-sampled study streams may aid with estimates of CPOM transport and export in less well-sampled Rocky Mountain streams. Transport relations for CPOM were similar among three high elevation mountain stream with mainly coniferous watersheds. Differences among streams can be qualitatively attributed to differences in CPOM contributions from litter fall, from the presence of large woody debris, its grinding into CPOM sized particles by gravel-cobble bedload transport, hillslope connectivity, drainage density, and biological consumption. CPOM loads were 3.6 and 3.2 t/yr for the two Rocky. Mountain streams. Adjusted to reflect decadal averages, values increased to 11.3 and 10.2 thin CPOM yields related to the entire watershed were 2.7 and 4 kg/ha/yr for the years studied, but both streams exported similar amounts of 6.5 and 6.6 kg/ha/yr when taking the forested portion of the watershed into account. To reflect decadal averages, CPOM yields per basin area were adjusted to 8.6 and 12.6 kg/ha/yr and to 21 kg/ha/yr for the forested watershed parts. CPOM yield may be more meaningfully characterized if annual CPOM loads are normalized by the area of a seam along the stream banks together with the stream surface area rather than by the forested or total watershed area. (C) 2016 Elsevier B.V. All rights reserved. C1 [Bunte, Kristin; Abt, Steven R.] Colorado State Univ, Engn Res Ctr, 1320 Campus Delivery, Ft Collins, CO 80523 USA. [Swingle, Kurt W.] 630 Iris Ave, Boulder, CO 80304 USA. [Turowski, Jens M.] Swiss Fed Res Inst WSL, Mt Hydrol & Mass Movements, Zuricherstr 111, CH-8903 Birmensdorf, Switzerland. [Cenderelli, Daniel A.] US Forest Serv, Natl Stream & Aquat Ecol Ctr, USDA, 2150 Ctr Ave, Ft Collins, CO 80523 USA. [Turowski, Jens M.] GFZ German Res Ctr Geosci, Helmholtz Ctr Potsdam, D-14473 Potsdam, Germany. RP Bunte, K (reprint author), Colorado State Univ, Engn Res Ctr, 1320 Campus Delivery, Ft Collins, CO 80523 USA. EM kbunte@engr.colostate.edu; kskb@ix.netcom.com; jens.turowski@gfz-potsdam.de; sabt@engr.colostate.edu; dcenderelli@fs.fed.us RI Turowski, Jens/A-6629-2009 OI Turowski, Jens/0000-0003-1558-0565 FU USDA Forest Service; Stream Systems Technology Center; National Stream and Aquatic Ecology Center, Fort Collins, CO; Swiss Federal Research Institute (WSL) in Birmensdorf, Switzerland FX We thank Sandra Ryan and Mark Dixon for the 2002 flow data from Little Granite Creek and L. Porth, K. Elder, and B. Starr (all USDA Forest Service, Rocky Mountain Research Station, Fort Collins, CO) for the 2001 East St. Louis Creek flow data. Field work, part of the data analysis, and part of the manuscript preparations was funded by the USDA Forest Service, Stream Systems Technology Center as well as by its successor the National Stream and Aquatic Ecology Center, Fort Collins, CO. Further analyses and part of the manuscript were prepared while K.B. received support from the Swiss Federal Research Institute (WSL) in Birmensdorf, Switzerland. Sandra Ryan (USFS), Marie Garsjo (ret., NRCS), and Ellen Wohl (CSU) provided friendly reviews of earlier versions of the manuscript. We thank John Potyondy (USFS ret.) for vision, insight, and long-term support of our field studies. NR 68 TC 0 Z9 0 U1 12 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG PY 2016 VL 539 BP 162 EP 176 DI 10.1016/j.jhydrol.2016.05.022 PG 15 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA DQ1IC UT WOS:000378953700012 ER PT J AU Hao, L Xie, JP Chen, SY Wang, SJ Gong, ZQ Ling, KS Guo, LY Fan, ZF Zhou, T AF Hao, Lu Xie, Jipeng Chen, Shanyi Wang, Shaojie Gong, Zhuoqun Ling, Kai-Shu Guo, Liyun Fan, Zaifeng Zhou, Tao TI A multiple RT-PCR assay for simultaneous detection and differentiation of latent viruses and apscarviroids in apple trees SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Apple Chlorotic leaf spot virus (ACLSV); Apple stem grooving virus (ASGV); Apple stem pitting virus (ASPV); Apscarviroid; Apple scar skin viroid (ASSVd); Certification program ID CHLOROTIC-LEAF-SPOT; STEM GROOVING VIRUS; PLANT MESSENGER-RNA; MOLECULAR VARIABILITY; RECOMBINATION EVENTS; INTERNAL CONTROL; FRUIT VIRUSES; IDENTIFICATION; PEAR; PATHOGENS AB Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), and Apple stem pitting virus (ASPV) are three latent viruses frequently occurring in apple trees worldwide. In field orchards, these viruses are frequently found in a mixed infection with viroids in the genus Apscarviroid, including Apple scar skin viroid, and Apple dimple fruit viroid. Together these viruses and viroids could cause serious damage to apple fruit production worldwide. Rapid and efficient detection methods are pivotal to identify and select the virus-free propagation material for healthy apple orchard management. In this study a multiplex Reverse Transcription-PCR (RT-PCR) was developed and optimized for simultaneous detection and differentiation of the three latent viruses and apscarviroids. With newly designed specific primers for ACLSV, ASGV, APSV, and EF-1 alpha (as an internal control), and a pair of degenerate primers for apscarviroids, optimized parameters for multiplex RT-PCR were determined. The resulting PCR products from each target virus and viroid could be easily identified because their product sizes differ by at least a 100 bp. The multiplex RT-PCR method is expected to detect different variants of the viruses as the test results showed that a variety of isolates from different regions in China gave positive results. To the best of our knowledge, this multiplex RT-PCR assay is the first to simultaneously detect multiple viruses and viroids infecting apple trees in a single reaction tube. This assay, therefore, offers a useful tool for routine certification and quarantine programs. (C) 2016 Elsevier B.V. All rights reserved. C1 [Hao, Lu; Xie, Jipeng; Chen, Shanyi; Wang, Shaojie; Gong, Zhuoqun; Guo, Liyun; Fan, Zaifeng; Zhou, Tao] China Agr Univ, State Key Lab Agrobiotechnol, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China. [Hao, Lu; Xie, Jipeng; Chen, Shanyi; Wang, Shaojie; Gong, Zhuoqun; Guo, Liyun; Fan, Zaifeng; Zhou, Tao] China Agr Univ, Dept Plant Pathol, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China. [Ling, Kai-Shu] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Zhou, T (reprint author), China Agr Univ, State Key Lab Agrobiotechnol, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China.; Zhou, T (reprint author), China Agr Univ, Dept Plant Pathol, 2 West Yuanmingyuan Rd, Beijing 100193, Peoples R China. EM taozhoucau@cau.edu.cn FU Earmarked Fund for Modern Agro-industry Technology Research System [CARS-28]; National High Technology Research and Development Program (863) of China [2012AA101501]; Special Fund for Agro-Scientific Research in the Public Interest [201203076-02] FX This research was supported by the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-28), the National High Technology Research and Development Program (863) of China (2012AA101501), and Special Fund for Agro-Scientific Research in the Public Interest (No. 201203076-02) administered by the Chinese Ministry of Agriculture. We thank Mr. Xudong Liu (Pomology Institute of Changping, Beijing, China) and Dr. Lingling Zhao (Pomology Institute of Yantai, Shandong province, China), Mr. Guoqiang Zhang (Yuncheng City, Shanxi province, China), and Mr. Jun Ma (Yunnan Academy of Agriculture Sciences, Kunming, Yunnan province, China) for kindly providing apple samples. NR 45 TC 0 Z9 0 U1 17 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD AUG PY 2016 VL 234 BP 16 EP 21 DI 10.1016/j.jviromet.2016.04.003 PG 6 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA DQ1MC UT WOS:000378964100003 PM 27054889 ER PT J AU Maiya, R McMahon, T Wang, D Kanter, B Gandhi, D Chapman, HL Miller, J Messing, RO AF Maiya, Rajani McMahon, Thomas Wang, Dan Kanter, Benjamin Gandhi, Dev Chapman, Holly L. Miller, Jacklyn Messing, Robert O. TI Selective chemical genetic inhibition of protein kinase C epsilon reduces ethanol consumption in mice SO NEUROPHARMACOLOGY LA English DT Article DE Protein kinase C; Alcohol; Ethanol; 1-NA-PP1; AS-Kinase ID PKC-EPSILON; C57BL/6J MICE; ALCOHOL; SENSITIVITY; SUPERSENSITIVITY; PHOSPHORYLATION; IDENTIFICATION AB Reducing expression or inhibiting translocation of protein kinase C epsilon (PKC epsilon) prolongs ethanol intoxication and decreases ethanol consumption in mice. However, we do not know if this phenotype is due to reduced PKC epsilon kinase activity or to impairment of kinase-independent functions. In this study, we used a chemical-genetic strategy to determine whether a potent and highly selective inhibitor of PKC epsilon catalytic activity reduces ethanol consumption. We generated ATP analog-specific PKC epsilon (AS-PKC epsilon) knock in mice harboring a point mutation in the ATP binding site of PKC epsilon that renders the mutant kinase highly sensitive to inhibition by 1-tert-butyl-3-naphthalen-1-ylpyrazolo[3,4-d]pyrimidin-4-amine (1-NA-PP1). Systemically administered 1-NA-PP1 readily crossed the blood brain barrier and inhibited PKC epsilon-mediated phosphorylation. 1-NA-PP1 reversibly reduced ethanol consumption by AS-PKC epsilon mice but not by wild type mice lacking the AS-PKC epsilon mutation. These results support the development of inhibitors of PKC epsilon Catalytic activity as a strategy to reduce ethanol consumption, and they demonstrate that the AS- PKC epsilon mouse is a useful tool to study the role of PKC epsilon in behavior. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Maiya, Rajani; Gandhi, Dev; Chapman, Holly L.; Messing, Robert O.] Univ Texas Austin, Coll Pharm, Div Pharmacol & Toxicol, Austin, TX 78712 USA. [McMahon, Thomas; Wang, Dan; Kanter, Benjamin; Miller, Jacklyn; Messing, Robert O.] Univ Calif San Francisco, Ernest Gallo Clin & Res Ctr, 5858 Horton St,Suite 200, Emeryville, CA 94608 USA. [McMahon, Thomas] UCSF Neurol, Box 2911,1550 4th St,Bldg 19B Room 546, San Francisco, CA 94158 USA. [Wang, Dan] UCSF Neurol, Box 1207,675 Nelson Rising Lane,Room 292, San Francisco, CA 94158 USA. [Kanter, Benjamin] Norwegian Univ Sci & Technol, Kavli Inst Syst Neurosci, MTFS, Olav Kyrres Gate 9, N-7489 Trondheim, Norway. [Kanter, Benjamin] Norwegian Univ Sci & Technol, Ctr Neural Computat, MTFS, Olav Kyrres Gate 9, N-7489 Trondheim, Norway. [Chapman, Holly L.] ARS, USDA, Foodborne Toxin Detect & Prevent, 800 Buchanan St, Albany, CA 94710 USA. RP Messing, RO (reprint author), UT Austin Coll Pharm, C0875,107 W Dean Keeton,BME6-116A, Austin, TX 78712 USA. EM romessing@austin.utexas.edu FU NIH [AA13588, AA017072]; State of California for medical research for alcohol and substance abuse through UCSF FX This work was supported by NIH grants AA13588 and AA017072, and by funds provided by the State of California for medical research for alcohol and substance abuse through UCSF to R.O.M. We thank Michael Cameron and the DMPK core at Scripps Florida and Yong Huang and the Drug Studies Unit, Analytical Division, UCSF College of Pharmacy for their work on the pharmacokinetics and tissue measurement of 1-NA-PP1. NR 25 TC 0 Z9 0 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0028-3908 EI 1873-7064 J9 NEUROPHARMACOLOGY JI Neuropharmacology PD AUG PY 2016 VL 107 BP 40 EP 48 DI 10.1016/j.neuropharm.2016.02.036 PG 9 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA DQ1HZ UT WOS:000378953400005 PM 26947945 ER PT J AU Wach, M Hellmich, RL Layton, R Romeis, J Gadaleta, PG AF Wach, Michael Hellmich, Richard L. Layton, Raymond Romeis, Jorg Gadaleta, Patricia G. TI Dynamic role and importance of surrogate species for assessing potential adverse environmental impacts of genetically engineered insect-resistant plants on non-target organisms SO TRANSGENIC RESEARCH LA English DT Article DE Surrogate species; Genetically engineered insect resistance; Environmental risk assessment ID TRANSGENIC CROPS; RISK ASSESSMENTS; METAANALYSIS; ARTHROPODS; PESTICIDES; MAIZE; FIELD AB Surrogate species have a long history of use in research and regulatory settings to understand the potentially harmful effects of toxic substances including pesticides. More recently, surrogate species have been used to evaluate the potential effects of proteins contained in genetically engineered insect resistant (GEIR) crops. Species commonly used in GEIR crop testing include beneficial organisms such as honeybees, arthropod predators, and parasitoids. The choice of appropriate surrogates is influenced by scientific factors such as the knowledge of the mode of action and the spectrum of activity as well as societal factors such as protection goals that assign value to certain ecosystem services such as pollination or pest control. The primary reasons for using surrogates include the inability to test all possible organisms, the restrictions on using certain organisms in testing (e.g., rare, threatened, or endangered species), and the ability to achieve greater sensitivity and statistical power by using laboratory testing of certain species. The acceptance of surrogate species data can allow results from one region to be applied or "transported" for use in another region. On the basis of over a decade of using surrogate species to evaluate potential effects of GEIR crops, it appears that the current surrogates have worked well to predict effects of GEIR crops that have been developed (Carstens et al. GM Crops Food 5:1-5, 2014), and it is expected that they should work well to predict effects of future GEIR crops based on similar technologies. C1 [Wach, Michael] ILSI Res Fdn, Ctr Environm Risk Assessment, Washington, DC 20005 USA. [Hellmich, Richard L.] Iowa State Univ, Corn Insects & Crop Genet Res Unit, USDA ARS, Ames, IA USA. [Hellmich, Richard L.] Iowa State Univ, Dept Entomol, Ames, IA USA. [Layton, Raymond] DuPont Pioneer, Johnston, IA USA. [Romeis, Jorg] Agroscope Reckenholz Tanikon Res Stn ART, Zurich, Switzerland. [Gadaleta, Patricia G.] Minist Agr Livestock & Fisheries, Biotechnol Directorate, Buenos Aires, DF, Argentina. RP Wach, M (reprint author), ILSI Res Fdn, Ctr Environm Risk Assessment, Washington, DC 20005 USA. EM mwach@ilsi.org OI Wach, Michael/0000-0001-7151-0727 NR 52 TC 2 Z9 2 U1 9 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD AUG PY 2016 VL 25 IS 4 BP 499 EP 505 DI 10.1007/s11248-016-9945-5 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA DQ2JJ UT WOS:000379028300009 PM 26922585 ER PT J AU Arikan, OA Mulbry, W Rice, C AF Arikan, Osman A. Mulbry, Walter Rice, Clifford TI The effect of composting on the persistence of four ionophores in dairy manure and poultry litter SO WASTE MANAGEMENT LA English DT Article DE Ionophore; Composting; Temperature; Dairy manure; Poultry litter ID CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHY; VETERINARY PHARMACEUTICALS; SURFACE-WATER; BEEF MANURE; MONENSIN; DEGRADATION; ANTIBIOTICS; SOIL AB Manure composting is a well-described approach for stabilization of nutrients and reduction of pathogens and odors. Although composting studies have shown that thermophilic temperatures and aerobic conditions can increase removal rates of selected antibiotics, comparable information is lacking for many other compounds in untreated or composted manure. The objective of this study was to determine the relative effectiveness of composting conditions to reduce concentrations of four widely used ionophore feed supplements in dairy manure and poultry litter. Replicate aliquots of fresh poultry litter and dairy manure were amended with monensin, lasalocid, salinomycin, or amprolium to 10 mg kg(-1) DW. Non-amended and amended dairy manure and poultry litter aliquots were incubated at 22, 45, 55, or 65 degrees C under moist, aerobic conditions. Residue concentrations were determined from aliquots removed after 1, 2, 4, 6, 8, and 12 weeks. Results suggest that the effectiveness of composting for contaminant reduction is compound and matrix specific. Composting temperatures were not any more effective than ambient temperature in increasing the rate or extent of monensin removal in either poultry litter or dairy manure. Composting was effective for lasalocid removal in poultry litter, but is likely to be too slow to be useful in practice (8-12 weeks at 65 degrees C for >90% residue removal). Composting was effective for amprolium removal from poultry litter and salinomycin in dairy manure but both required 4-6 weeks for >90% removal. However, composting did not increase the removal rates or salinomycin in poultry litter or the removal rates of lasalocid or amprolium in dairy manure. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Arikan, Osman A.; Mulbry, Walter; Rice, Clifford] USDA ARS, Beltsville Agr Res Ctr, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Arikan, Osman A.] Istanbul Tech Univ, Dept Environm Engn, TR-34469 Istanbul, Turkey. RP Arikan, OA (reprint author), Istanbul Tech Univ, Dept Environm Engn, TR-34469 Istanbul, Turkey. EM arikan@itu.edu.tr RI ARIKAN, OSMAN/F-1193-2015 NR 39 TC 1 Z9 1 U1 25 U2 40 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0956-053X J9 WASTE MANAGE JI Waste Manage. PD AUG PY 2016 VL 54 BP 110 EP 117 DI 10.1016/j.wasman.2016.04.032 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA DQ1LM UT WOS:000378962500013 PM 27189139 ER PT J AU Siriwardena, SD Boyle, KJ Holmes, TP Wiseman, PE AF Siriwardena, Shyamani D. Boyle, Kevin J. Holmes, Thomas P. Wiseman, P. Eric TI The implicit value of tree cover in the US: A meta-analysis of hedonic property value studies SO ECOLOGICAL ECONOMICS LA English DT Article DE Hedonic valuation; Tree canopy cover; Community forestry; Forest matrix; Climate change ID CLIMATE-CHANGE; UNITED-STATES; FORESTS; SPACE; GREEN AB Trees in residential neighborhoods and communities provide benefits for homeowners that are capitalized into residential property values. In this paper, we collected data from hedonic property value studies and merged these data with ancillary spatial data describing forest and socio-economic characteristics surrounding each study area to conduct a meta-analysis of the impact of tree canopy cover on the value of residential properties. The meta-analysis suggests that property-level tree cover of about 30% and county-level tree cover of about 38% maximize the implicit price of tree cover in property values. Currently, tree cover in the original study areas was about 14%, on average, around or near study properties. The empirical results, therefore suggest under investment of tree cover on private property from the perspective of individual property owners and from a societal perspective. The findings also have implications for community forest programs regarding planting trees and protection of mature trees to address potential changes in tree abundance, species diversity and stand age due to development and climate change. (C) 2016 Elsevier B.V. All rights reserved. C1 [Siriwardena, Shyamani D.] Virginia Tech, Dept Forest Resources & Environm Conservat, 313 Cheatham Hall 0324, Blacksburg, VA 24061 USA. [Boyle, Kevin J.] Virginia Tech, Virginia Tech Program Real Estate, 420 Bishop Favrao Hall 0715, Blacksburg, VA USA. [Holmes, Thomas P.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC USA. [Wiseman, P. Eric] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. [Siriwardena, Shyamani D.; Boyle, Kevin J.; Wiseman, P. Eric] Virginia Tech, Blacksburg, VA USA. [Holmes, Thomas P.] US Forest Serv, Washington, DC USA. RP Siriwardena, SD (reprint author), Virginia Tech, Dept Forest Resources & Environm Conservat, 313 Cheatham Hall 0324, Blacksburg, VA 24061 USA. EM shyadil@vt.edu; kjboyle@vt.edu; tholmes@fs.fed.us; pwiseman@vt.edu RI Wiseman, Eric/E-2336-2017 OI Wiseman, Eric/0000-0002-5743-7612 FU Southern Research Station of the U.S. Forest Service [12-JV-11330143-106]; Virginia Agricultural Experimental Station FX This research was supported by the Southern Research Station of the U.S. Forest Service (12-JV-11330143-106) and Virginia Agricultural Experimental Station. NR 40 TC 1 Z9 1 U1 6 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8009 EI 1873-6106 J9 ECOL ECON JI Ecol. Econ. PD AUG PY 2016 VL 128 BP 68 EP 76 DI 10.1016/j.ecolecon.2016.04.016 PG 9 WC Ecology; Economics; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology; Business & Economics GA DP7HL UT WOS:000378669700008 ER PT J AU Benda, LE Litschert, SE Reeves, G Pabst, R AF Benda, Lee E. Litschert, S. E. Reeves, Gordon Pabst, Robert TI Thinning and in-stream wood recruitment in riparian second growth forests in coastal Oregon and the use of buffers and tree tipping as mitigation SO JOURNAL OF FORESTRY RESEARCH LA English DT Article DE Forest management; Thinning; Riparian; Woody debris; Forestry; Watersheds; Fish habitat ID WESTERN OREGON; COHO SALMON; DEBRIS; MANAGEMENT; MODEL; VARIABILITY; TRANSPORT; SIMULATOR; DYNAMICS; PATTERNS AB Many aquatic habitats in coastal Oregon have been impacted by historic land use practices that led to losses of in-stream wood and associated degraded fish habitats. Many of these streams are now bordered by stands of dense second growth forests (30-80 years) that are incorporated into riparian buffer zones with low wood recruitment and storage. Thinning in riparian zones is one management option to increase the rate of large tree growth and eventually larger in-stream wood, however, it raises concern about impacts on current wood recruitment, among other issues. Using a forest growth simulation model coupled to a model of in-stream wood recruitment, we explore riparian management alternatives in a Douglas-fir plantation in coastal Oregon. Alternatives included: (1) no treatment, (2) single and double entry thinning, without and with a 10-m buffer, and (3) thinning combined with mechanical introduction of some portion of the thinned trees into the stream (tree tipping). Compared to no treatment, single and double entry thinning on one side of a channel, without a 10-m buffer, reduce cumulative in-stream wood volume by 33 and 42 %, respectively, after 100 years (includes decay). Maintaining a 10-m buffer reduces the in-stream wood loss to 7 % (single entry thin) and 11 % (double entry). To completely offset the losses of in-stream wood in a single entry thin (on one or both sides of the stream), in the absence or presence of a 10-m buffer, requires a 12-14 % rate of tree tipping. Relative to the no-treatment alternative, cumulative in-stream wood storage can be increased up to 24 % in a double-entry thin with no buffer by tipping 15-20 % of the thinned trees (increased to 48 % if thinning and tipping simultaneously on both sides of the stream). The predicted increases in in-stream wood that can occur during a thin with tree tipping may be effective for restoring fish habitat, particularly in aquatic systems that have poor habitat conditions and low levels of in-stream wood due to historic land use activities. C1 [Benda, Lee E.] TerrainWorks, Mt Shasta, CA 96067 USA. [Litschert, S. E.] BLM Natl Operat Ctr, Denver, CO 80225 USA. [Reeves, Gordon] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Pabst, Robert] Oregon State Univ, Corvallis, OR 97331 USA. RP Benda, LE (reprint author), TerrainWorks, Mt Shasta, CA 96067 USA. EM leebenda@terrainworks.com FU U.S. Forest Service; Pacific Northwest Research Station; Earth Systems Institute, Seattle Washington FX This research was supported by the U.S. Forest Service, Pacific Northwest Research Station and Earth Systems Institute, Seattle Washington. NR 49 TC 1 Z9 1 U1 8 U2 9 PU NORTHEAST FORESTRY UNIV PI HARBIN PA NO 26 HEXING RD, XIANGFANG DISTRICT, HARBIN, 150040, PEOPLES R CHINA SN 1007-662X EI 1993-0607 J9 J FORESTRY RES JI J. For. Res. PD AUG PY 2016 VL 27 IS 4 BP 821 EP 836 DI 10.1007/s11676-015-0173-2 PG 16 WC Forestry SC Forestry GA DP9GX UT WOS:000378805800011 ER PT J AU Xie, JL Qi, JQ Hu, TX De Hoop, CF Hse, CY Shupe, TF AF Xie, Jiulong Qi, Jinqiu Hu, Tingxing De Hoop, Cornelis F. Hse, Chung Yun Shupe, Todd F. TI Effect of fabricated density and bamboo species on physical-mechanical properties of bamboo fiber bundle reinforced composites SO JOURNAL OF MATERIALS SCIENCE LA English DT Article ID ANATOMICAL CHARACTERISTICS; HEIGHT; LUMBER AB Bamboo stems were subjected to a mechanical treatment process for the extraction of bamboo fiber bundles. The fiber bundles were used as reinforcement for the fabrication of high-performance composites with phenolic resins as matrix. The influence of fabricated density and bamboo species on physical-mechanical properties of bamboo fiber bundle reinforced composites (BFCs) was evaluated. The results revealed that BFCs with density of 1200 kg/m(3) exhibited lower water absorption, better dimensional stability, and higher mechanical properties with comparison to those with lower density. The changes in microstructures of BFCs with respect to density gave evidence that the high performance of BFCs with high density was due to the almost complete collapse of bamboo lumens, which resulted in the formation of solid bamboo and thin resin films with water resistance ability. BFCs fabricated from five bamboo species all showed better properties compared to commercialized bamboo-based composites. However, significant differences in physical-mechanical properties of BFCs among bamboo species were also found. This may be attributed to the variations in anatomical structure and physical-mechanical properties among original bamboo species. From a practical production view, the effect of bamboo species on properties of BFCs should be properly taken into consideration. C1 [Xie, Jiulong; Qi, Jinqiu; Hu, Tingxing] Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Peoples R China. [De Hoop, Cornelis F.; Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Hse, Chung Yun] US Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. RP Qi, JQ (reprint author), Sichuan Agr Univ, Coll Forestry, Chengdu 611130, Peoples R China. EM qijinqiu2005@aliyun.com FU Key Laboratory of Wood Industry and Furniture Engineering of Sichuan Provincial Colleges and Universities FX This work has been supported by "Key Laboratory of Wood Industry and Furniture Engineering of Sichuan Provincial Colleges and Universities." NR 33 TC 0 Z9 0 U1 6 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0022-2461 EI 1573-4803 J9 J MATER SCI JI J. Mater. Sci. PD AUG PY 2016 VL 51 IS 16 BP 7480 EP 7490 DI 10.1007/s10853-016-0024-3 PG 11 WC Materials Science, Multidisciplinary SC Materials Science GA DP5MS UT WOS:000378542300009 ER PT J AU You, Q Pan, YB Xu, LP Gao, SW Wang, QN Su, YC Yang, YQ Wu, QB Zhou, DG Que, YX AF You, Qian Pan, Yong-Bao Xu, Li-Ping Gao, Shi-Wu Wang, Qin-Nan Su, Ya-Chun Yang, Yong-Qing Wu, Qi-Bin Zhou, Ding-Gang Que, You-Xiong TI Genetic Diversity Analysis of Sugarcane Germplasm Based on Fluorescence-Labeled Simple Sequence Repeat Markers and a Capillary Electrophoresis-based Genotyping Platform SO SUGAR TECH LA English DT Article DE Sugarcane; Breeding germplasm; SSR; Capillary electrophoresis; Genetic diversity; Diversity index ID EST-SSR MARKERS; MICROSATELLITE MARKERS; AFLP ANALYSIS; TRAP MARKERS; SACCHARUM; POPULATIONS; LANDRACES; CULTIVAR; SORGHUM; UTILITY AB Genetic diversity analysis, which refers to the elaboration of total extent of genetic characteristics in the genetic makeup of a certain species, constitutes a classical strategy for the study of diversity, population genetic structure, and breeding practices. In this study, fluorescence-labeled seven gSSR and eight EST-SSR primer pairs and a capillary electrophoresis genotyping platform were used to assess the genetic diversity among 181 sugarcane clones. The clones were sorted into 14 series based on their origin. A total of 205 polymorphic SSR alleles were identified. The mean polymorphic information content (PIC) value was 0.94 for gSSRs and 0.93 for EST-SSRs, respectively. Gene differentiation coefficient (Gst) of inter-series variation (13.71 %) was much lower than intra-series variation (86.29 %). Gene flow value (Nm = 3.15) suggested that there was no significant genetic differentiation or population structure variations among the 14 series. The 181 clones could be clustered into seven groups based on neighbor-joining cluster analysis. Three major groups, namely the USA Group, the Guangxi-Hainan-Fujian Group, and the Guangdong Group, consisted of 36, 64, and 39 clones, respectively. The genotyping data provide valuable information for selecting cross parents, designing cross combinations, and future hybrid breeding strategies. C1 [You, Qian; Xu, Li-Ping; Gao, Shi-Wu; Su, Ya-Chun; Yang, Yong-Qing; Wu, Qi-Bin; Zhou, Ding-Gang; Que, You-Xiong] Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou, Fujian, Peoples R China. [Pan, Yong-Bao] USDA ARS, Sugarcane Res Lab, Houma, LA 70360 USA. [Wang, Qin-Nan] Guangzhou Sugarcane Ind Res Inst, Hainan Sugarcane Breeding Stn, Sanya, Hainan, Peoples R China. RP Xu, LP; Que, YX (reprint author), Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr, Fuzhou, Fujian, Peoples R China. EM xlpmail@126.com; queyouxiong@126.com FU earmarked fund for the Modern Agriculture Technology of China [CARS-20]; 948 Program on the Introduction of International Advanced Agricultural Science and Technique of Department of Agriculture [2014-S18] FX This work was supported by the earmarked fund for the Modern Agriculture Technology of China (CARS-20) and the 948 Program on the Introduction of International Advanced Agricultural Science and Technique of Department of Agriculture (2014-S18). The authors especially thank Andrew C. Allan in The New Zealand Institute for Plant & Food Research Ltd. (Plant and Food Research), Mt Albert Research Centre, Auckland, New Zealand, for his valuable comments and support during paper preparation. NR 60 TC 0 Z9 0 U1 7 U2 16 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0972-1525 J9 SUGAR TECH JI Sugar Tech. PD AUG PY 2016 VL 18 IS 4 BP 380 EP 390 DI 10.1007/s12355-015-0395-9 PG 11 WC Agronomy SC Agriculture GA DP7XF UT WOS:000378711800007 ER PT J AU McCord, PH Migneault, AJ AF McCord, Per H. Migneault, Andrew J. TI Genotyping Sugarcane for the Brown Rust Resistance Locus Bru1 Using Unlabeled Probe Melting SO SUGAR TECH LA English DT Article DE Brown rust; Bru1; Sugarcane; Unlabeled probe melting ID GENE BRU1; CULTIVARS AB Brown rust, caused by the fungus Puccinia melanocephala, is a major disease of sugarcane (Saccharum spp. hybrid) in Florida, Louisiana, and other sugarcane growing regions. The Bru1 locus has been used as a durable and effective source of resistance, and markers are available to select for the trait. The markers currently being used by the USDA Sugarcane Field Station in Canal Point, FL for Bru1 genotyping have two disadvantages. One marker (here Bru1B) is dominant, which means that a Bru1-negative individual cannot be distinguished from a failed PCR reaction. The second marker (here Bru1A) is codominant, but genotyping requires enzyme restriction and gel electrophoresis, adding time and cost to the analysis. A closed-tube, codominant assay for Bru1 would significantly decrease the time and cost currently required for Bru1 genotyping. By sequencing the Bru1A PCR product from Bru1-positive and Bru1-negative individuals, we indentified two SNPs (here Bru1A1 and Bru1A2) that alter the relevant restriction enzyme recognition sequence. An unlabeled probe assay was designed to target each of the SNPs. Unlabeled probe assays are DNA melting assays that rely on the dissociation of an oligonucleotide probe from its target DNA strand. The dissociation is detected by the decrease in fluorescence of double-stranded DNA binding dyes and is sensitive enough to detect single base pair changes. A comparison between the traditional genotyping method and unlabeled probe melting using 344 genotypes showed a 94.7 % success rate, with 100 % concordance between Bru1A and unlabeled probe genotyping. We also confirmed prior unpublished results that discovered a rare recombination between Bru1A and Bru1B. We have demonstrated that unlabeled probe melting can be used to detect the Bru1 locus, eliminates the risk of false negatives, and is faster and less expensive than the current method. C1 [McCord, Per H.] USDA ARS, Sugarcane Field Stn, 12990 Highway 441, Canal Point, FL 33438 USA. [Migneault, Andrew J.] Univ Florida, Everglades Res & Educ Ctr, 3200 East Palm Beach Rd, Belle Glade, FL 33430 USA. RP McCord, PH (reprint author), USDA ARS, Sugarcane Field Stn, 12990 Highway 441, Canal Point, FL 33438 USA. EM per.mccord@ars.usda.gov NR 15 TC 1 Z9 1 U1 3 U2 6 PU SPRINGER INDIA PI NEW DELHI PA 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI, 110 001, INDIA SN 0972-1525 J9 SUGAR TECH JI Sugar Tech. PD AUG PY 2016 VL 18 IS 4 BP 401 EP 406 DI 10.1007/s12355-015-0390-1 PG 6 WC Agronomy SC Agriculture GA DP7XF UT WOS:000378711800009 ER PT J AU Rai, DK Segundo, FDS Schafer, E Burrage, TG Rodriguez, LL de los Santos, T Hoeprich, PD Rieder, E AF Rai, Devendra K. Segundo, Fayna Diaz-San Schafer, Elizabeth Burrage, Thomas G. Rodriguez, Luis L. de los Santos, Teresa Hoeprich, Paul D. Rieder, Elizabeth TI Novel 6xHis tagged foot-and-mouth disease virus vaccine bound to nanolipoprotein adjuvant via metal ions provides antigenic distinction and effective protective immunity SO VIROLOGY LA English DT Article DE FMDV; 6xHis Tag; FMD vaccine; Nanolipoprotein adjuvants ID VP1/2A JUNCTION; PARTICLES; PROTEIN; FMDV; ANTIBODIES; CATTLE; PURIFICATION; PATHOGENESIS; ERADICATION; DELIVERY AB Here, we engineered two FMD viruses with histidine residues inserted into or fused to the FMDV capsid. Both 6xHis viruses exhibited growth kinetics, plaque morphologies and antigenic characteristics similar to wild-type virus. The 6xHis tag allowed one-step purification of the mutant virions by Co2+ affinity columns. Electron microscopy and biochemical assays showed that the 6xHis FMDV5 readily assembled into antigen: adjuvant complexes in solution, by conjugating with Ni2+-chelated nanolipoprotein and monophosphoryl lipid A adjuvant (MPLA:NiNLP). Animals Immunized with the inactivated 6xHis-FMDV: MPLA:NiNLP vaccine acquired enhanced protective immunity against FMDV challenge compared to virions alone. Induction of anti-6xHis and anti-FMDV neutralizing antibodies in the immunized animals could be exploited in the differentiation of vaccinated from infected animals needed for the improvement of FMD control measures. The novel marker vaccine/nanolipid technology described here has broad applications for the development of distinctive and effective immune responses to other pathogens of importance. Published by Elsevier Inc. C1 [Rai, Devendra K.; Segundo, Fayna Diaz-San; Schafer, Elizabeth; Rodriguez, Luis L.; de los Santos, Teresa; Rieder, Elizabeth] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. [Rai, Devendra K.; Segundo, Fayna Diaz-San] Univ Connecticut, CANR, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA. [Burrage, Thomas G.] ARS, Dept Homeland Secur, S&T Targeted Adv Dev Virus Cellular & Mol Imaging, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Hoeprich, Paul D.] Lawrence Livermore Natl Lab, Phys & Life Sci Directorate, Livermore, CA USA. RP Rieder, E (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, NAA, POB 848, Greenport, NY 11944 USA. EM Elizabeth.Rieder@ars.usda.gov FU CRIS, Agricultural Research Service (ARS), U.S. Department of Agriculture [1940-32000-057-00D] FX Funding for the research detailed in this manuscript was provided through Congressionally allocated dollars for the Agricultural Research Service of the United States Department of Agriculture. Specifically, CRIS projects no. 1940-32000-057-00D, Agricultural Research Service (ARS), U.S. Department of Agriculture (Dr. Elizabeth Rieder). NR 35 TC 0 Z9 0 U1 5 U2 7 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD AUG PY 2016 VL 495 BP 136 EP 147 DI 10.1016/j.virol.2016.04.027 PG 12 WC Virology SC Virology GA DP7DR UT WOS:000378659900015 PM 27209448 ER PT J AU Bosco-Lauth, AM Bowen, RA Root, JJ AF Bosco-Lauth, Angela M. Bowen, Richard A. Root, J. Jeffrey TI Limited transmission of emergent H7N9 influenza A virus in a simulated live animal market: Do chickens pose the principal transmission threat? SO VIROLOGY LA English DT Article DE Live animal market; Influenza A virus; H7N9; Chicken; Quail; Pheasant; Rabbit; Pigeon; House sparrow; Transmission ID AVIAN INFLUENZA; BIRD MARKETS; WET MARKETS; POULTRY; INFECTION; FERRETS; PIGEONS; H5N1; ANTIBODIES; CHINA AB Emergent H7N9 influenza A virus has caused multiple public health and financial hardships. While some epidemiological studies have recognized infected chickens as an important bridge for human infections, the generality of this observation, the minimum infectious dose, and the shedding potential of chickens have received conflicting results. We experimentally tested the ability of domestic chickens (Gallus gallus domesticus) to transmit H7N9 to co-housed chickens and to several other animal species in an experimental live animal market. Results indicated that an infected chicken failed to initiate viral shedding of H7N9 to naive co-housed chickens. The infected chicken did, however, successfully transmit the virus to quail (Coturnix sp.) located directly below the infected chicken cage. Oral shedding by indirectly infected quail was, on average, greater than ten-fold that of directly inoculated chickens. Best management practices in live animal market systems should consider the position of quail in stacked-cage settings. (C) 2016 Elsevier Inc. All rights reserved. C1 [Bosco-Lauth, Angela M.; Bowen, Richard A.] Colorado State Univ, Dept Biomed Sci, Ft Collins, CO 80523 USA. [Root, J. Jeffrey] USDA, Natl Wildlife Res Ctr, Ft Collins, CO USA. RP Bosco-Lauth, AM (reprint author), Colorado State Univ, Dept Biomed Sci, Ft Collins, CO 80523 USA. EM mopargal@rams.colostate.edu FU USDA [7408-0983-CAUSDA] FX We thank the National Wildlife Research Center (NWRC) animal care staff for excellent assistance, J. Ellis and L. Peterson (NWRC) and R. Putnam Colorado State University (CSU) for assistance with animal husbandry, and J. Ellis for assistance with figure preparation. The opinions and conclusions of this article are those of the authors and do necessarily represent those of the U.S. Department of Agriculture (USDA) or Colorado State University. The mention of commercial products herein is for identification purposes only and does not constitute endorsement or censure. Funding was provided by the USDA (Cooperative agreement number 7408-0983-CAUSDA). NR 27 TC 1 Z9 1 U1 3 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD AUG PY 2016 VL 495 BP 161 EP 166 DI 10.1016/j.viro1.2016.04.032 PG 6 WC Virology SC Virology GA DP7DR UT WOS:000378659900017 PM 27236304 ER PT J AU Stoklosa, AM Ulyshen, MD Fan, ZF Varner, M Seibold, S Muller, J AF Stoklosa, Allison M. Ulyshen, Michael D. Fan, Zhaofei Varner, Morgan Seibold, Sebastian Muller, Jorg TI Effects of mesh bag enclosure and termites on fine woody debris decomposition in a subtropical forest SO BASIC AND APPLIED ECOLOGY LA English DT Article DE Ecosystems services; Invertebrates; Isoptera; Litter bag; Reticulitermes; Saproxylic ID LITTER DECOMPOSITION; SIZE; RHINOTERMITIDAE; MICROARTHROPODS; COARSE; DECAY AB The role of insects in terrestrial decomposition remains poorly resolved, particularly for infrequently studied substrates like small diameter woody debris. Uncertainty about how mesh bags used to exclude arthropods may affect decomposition rates continues to impede progress in this area. We sought to (1) measure how insects affect the decomposition of small diameter (<2.5 cm) Triadica sebifera L. "twigs" and (2) test for unintended effects of mesh bags on wood decomposition using machined wooden dowels of similar size and specific gravity. In subtropical forests, three twig diameter classes plus dowels were enclosed in two mesh sizes (0.3 or 1 mm openings) or left unenclosed over a 20-month study period. Unenclosed twigs lost significantly more mass than those within tine mesh bags. Because this effect was consistent throughout the study (reaching nearly 80% mass loss), our findings suggest the invertebrate influence does not disappear or attenuate over time. Our dowel data (limited to dowels with no evidence of insect activity) show that fine and coarse mesh bags accelerate the decomposition of enclosed woody material, suggesting insects contributed even more to the decomposition of tallow twigs than our measure of 9 - 10%. Termites exhibited a strong preference for larger diameter twigs, resulting in temporary differences in decomposition rates among diameter classes. Our findings confirm the importance of insects to wood decomposition and highlight the need to incorporate these organisms in models of carbon and nutrient cycling. C1 [Stoklosa, Allison M.; Fan, Zhaofei; Varner, Morgan] Mississippi State Univ, Dept Forestry, Forest & Wildlife Res Ctr, Box 9681, Starkville, MS 39759 USA. [Stoklosa, Allison M.] Drexel Univ, Acad Nat Sci, Philadelphia, PA 19103 USA. [Ulyshen, Michael D.] US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. [Fan, Zhaofei] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Varner, Morgan] US Forest Serv, USDA, Pacific NW Res Stn, Seattle, WA 98103 USA. [Seibold, Sebastian; Muller, Jorg] Bavarian Forest Natl Pk, Freyunger Str 2, D-94481 Grafenau, Germany. [Seibold, Sebastian; Muller, Jorg] Tech Univ Munich, Ctr Food & Life Sci Weihenstephan, Terr Ecol Res Grp, Dept Ecol & Ecosyst Management, Hans Carl von Carlowitz Pl 2, D-85354 Freising Weihenstephan, Germany. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, Southern Res Stn, Athens, GA 30602 USA. EM mulyshen@fs.fed.us FU Mississippi Forest and Wildlife Research Center; USDA Forest Service (Southern Research Station) FX We thank Nana Tian Mary Frances Nieminen, and Darcy Hammond for assisting with field work and the Statistical Consulting Center at the University of Georgia for assisting with the analysis. We also thank two anonymous reviewers for comments on an early draft of the manuscript. Finally, we are grateful to the Mississippi Forest and Wildlife Research Center and the USDA Forest Service (Southern Research Station) for providing financial and logistical support. NR 21 TC 1 Z9 1 U1 16 U2 19 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 1439-1791 EI 1618-0089 J9 BASIC APPL ECOL JI Basic Appl. Ecol. PD AUG PY 2016 VL 17 IS 5 BP 463 EP 470 DI 10.1016/j.baae.2016.03.001 PG 8 WC Ecology SC Environmental Sciences & Ecology GA DP0IB UT WOS:000378172100010 ER PT J AU Scally, M Into, F Thomas, DB Ruiz-Arce, R Barr, NB Schuenzel, EL AF Scally, M. Into, F. Thomas, D. B. Ruiz-Arce, R. Barr, N. B. Schuenzel, E. L. TI Resolution of inter and intra-species relationships of the West Indian fruit fly Anastrepha obliqua SO MOLECULAR PHYLOGENETICS AND EVOLUTION LA English DT Article DE Anastrepha species complex; Anastrepha fraterculus; Cryptic species; Multigene phylogeny ID FRATERCULUS COMPLEX DIPTERA; BAYESIAN PHYLOGENETIC INFERENCE; ANIMAL MITOCHONDRIAL-DNA; MAXIMUM-LIKELIHOOD; NEODIPRION HYMENOPTERA; SPECIES DELIMITATION; GENE FLOW; TEPHRITIDAE; INTROGRESSION; SEQUENCES AB The West Indian fruit fly, Anastrepha obliqua (Diptera: Tephritidae), is an economically important pest that inhabits areas of South and Central America, Mexico and the Caribbean with occasional infestations in the southern United States. We examine intra-specific relationships within A. obliqua as well as inter specific relationships to other Anastrepha species using a multi-locus data set comprising nine loci (seven nuclear, two mitochondrial) with 105 operational taxonomic units. The results based on a concatenated set of nuclear loci strongly support the monophyly of A. obliqua and most of the other species previously identified by morphology. A split between Peruvian A. obliqua samples and those from other locations was also identified. These results contrast with prior findings of relationships within A. obliqua based on mitochondrial data, as we found a marked discrepancy between nuclear and mitochondrial loci. These analyses suggest that introgression, particularly between A. obliqua and fraterculus species, may be one explanation for the discrepancy and the high mitochondrial diversity reported for A. obliqua could be the result of incomplete lineage sorting. (C) 2016 Elsevier Inc. All rights reserved. C1 [Scally, M.; Into, F.; Schuenzel, E. L.] Univ Texas Rio Grande Valley, Dept Biol, 1201 West Univ Dr, Edinburg, TX 78539 USA. [Thomas, D. B.] USDA ARS, Cattle Fever Tick Res Lab, Moore Air Base, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. [Ruiz-Arce, R.; Barr, N. B.] USDA APHIS Ctr Plant Hlth Sci & Technol, Moore Air Base, Mission Lab, 22675 N Moorefield Rd, Edinburg, TX 78541 USA. RP Schuenzel, EL (reprint author), Univ Texas Rio Grande Valley, Dept Biol, 1201 West Univ Dr, Edinburg, TX 78539 USA. EM mark.scally@utrgv.edu; fjibarra11@yahoo.com; Donald.Thomas@ars.usda.gov; Raul.A.Ruiz@aphis.usda.gov; Norman.B.Barr@aphis.usda.gov; erin.schuenzel@utrgv.edu NR 52 TC 0 Z9 0 U1 4 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1055-7903 EI 1095-9513 J9 MOL PHYLOGENET EVOL JI Mol. Phylogenet. Evol. PD AUG PY 2016 VL 101 BP 286 EP 293 DI 10.1016/j.ympev.2016.04.020 PG 8 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA DP0OC UT WOS:000378188100024 PM 27126185 ER PT J AU Timper, P Liu, C Davis, RF Wu, TH AF Timper, Patricia Liu, Chang Davis, Richard F. Wu, Tiehang TI Influence of crop production practices on Pasteuria penetrans and suppression of Meloidogyne incognita SO BIOLOGICAL CONTROL LA English DT Article DE Gossypium hirsutum; Nematode suppression; Meloidogyne incognita; Pasteuria penetrans; Root-knot nematode ID ROOT-KNOT NEMATODES; IN-FIELD SOIL; SOYBEAN FIELD; UNITED-STATES; FOOD-WEB; MANAGEMENT; TILLAGE; JAVANICA; ARENARIA; COMMUNITY AB Pasteuria penetrans is a parasite of root-knot nematodes (Meloidogyne spp.). Infected nematodes are not killed by the bacterium, but instead of producing eggs, females produce millions of infectious endospores. In addition to sterilizing females, P. penetrans can reduce nematode infection of roots when spore densities in soil are high because juveniles become so heavily encumbered, movement is restricted. A 4-year field study was conducted to determine 1) if fumigation with 1,3-dichloropropene (1,3-D, 28 L/ha) would have a negative effect on P. penetrans and general suppression of nematodes, 2) if the occasional use of 1,3-D would be as detrimental to P. penetrans as a yearly application, 3) if tillage influenced the abundance of P. penetrans spores, and 4) if the P. penetrans at the field site was contributing to suppression of M. incognita. Fumigation with 1,3-D reduced the abundance of P. penetrans spores in the soil compared to the no-fumigation control and there was no difference between a yearly application of the fumigant and occasional applications. Tillage (conventional and strip) did not affect spore abundance. The reduction of P. penetrans spores by fumigation was small compared to the year-to-year fluctuations in spore densities. Spores per assay nematode varied from 6.3 in 2012 to 0.8 in 2014. In 2012, P. penetrans appeared to suppress populations of M. incognita to very low levels (<= 10% of the root system with galls). The extreme changes in spore densities over the course of this study were likely due to a combination of factors including the density-dependent dynamics between the nematode and bacterium, the self-limiting effect of the bacterium at high spore densities, and leaching of spores out of the root zone during rain and irrigation events. Within each fumigation treatment, there was an inverse relationship (P <= 0.003, r = -0.40 to -0.58) between spore abundance and root galling indicating that the bioassay to estimate spore numbers in the spring was a good predictor of the level of nematode suppression. Published by Elsevier Inc. C1 [Timper, Patricia; Davis, Richard F.] USDA ARS, Crop Protect Management Res Unit, Tifton, GA 31793 USA. [Liu, Chang] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Wu, Tiehang] Georgia So Univ, Dept Biol, Statesboro, GA 30460 USA. RP Timper, P (reprint author), POB 748, Tifton, GA 31793 USA. EM patricia.timper@ars.usda.gov NR 41 TC 0 Z9 0 U1 5 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD AUG PY 2016 VL 99 BP 64 EP 71 DI 10.1016/j.biocontrol.2016.04.013 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA DO2IC UT WOS:000377602200009 ER PT J AU Virapongse, A Brooks, S Metcalf, EC Zedalis, M Gosz, J Kliskey, A Alessa, L AF Virapongse, Arika Brooks, Samantha Metcalf, Elizabeth Covelli Zedalis, Morgan Gosz, Jim Kliskey, Andrew Alessa, Lilian TI A social-ecological systems approach for environmental management SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Review DE Social-ecological systems; Environmental management; Natural resource management; Land management ID NATURAL-RESOURCE MANAGEMENT; TRANSDISCIPLINARY COLLABORATION; BIODIVERSITY CONSERVATION; RESILIENCE THINKING; COMANAGEMENT; SUSTAINABILITY; TRANSFORMATION; FOUNDATIONS; THRESHOLDS; GOVERNANCE AB Urgent environmental issues are testing the limits of current management approaches and pushing demand for innovative approaches that integrate across traditional disciplinary boundaries. Practitioners, scholars, and policy-makers alike call for increased integration of natural and social sciences to develop new approaches that address the range of ecological and societal impacts of modern environmental issues. From a theoretical perspective, social-ecological systems (SES) science offers a compelling approach for improved environmental management through the application of transdisciplinary and resilience concepts. A framework for translating SES theory into practice, however, is lacking. In this paper, we define the key components of an SES-based environmental management approach. We offer recommendations for integrating an SES approach into existing environmental management practices. Results presented are useful for management professionals that seek to employ an SES environmental management approach and scholars aiming to advance the theoretical foundations of SES science for practical application. Published by Elsevier Ltd. C1 [Virapongse, Arika; Kliskey, Andrew; Alessa, Lilian] Univ Idaho, Ctr Resilient Communities, Moscow, ID 83844 USA. [Virapongse, Arika] Univ Florida, Ctr Latin Amer Studies, Trop Conservat & Dev, Gainesville, FL 32611 USA. [Brooks, Samantha] Madison River Grp LW, Ashburn, VA 20148 USA. [Metcalf, Elizabeth Covelli] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59182 USA. [Zedalis, Morgan] US Forest Serv, Heritage Program, Payette Natl Forest, Mccall, ID 83638 USA. [Gosz, Jim] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA. [Alessa, Lilian] Univ Alaska Fairbanks, Int Arctic Res Ctr, Fairbanks, AK 99775 USA. RP Virapongse, A (reprint author), Univ Florida, Ctr Latin Amer Studies, Trop Conservat & Dev, Gainesville, FL 32611 USA. EM avirapongse@gmail.com OI Virapongse, Arika/0000-0002-0194-5618 FU Research Coordination Award for the Mountain Social Ecological Observatory Network (MtnSEON) [NSF-1231233]; National Science Foundation [BCS-1114851, OIA-1301792] FX This paper was inspired through discussions of the SESTEP working group that was supported through a Research Coordination Award for the Mountain Social Ecological Observatory Network (MtnSEON; NSF-1231233). Funding support was also made available through National Science Foundation grant BCS-1114851 and OIA-1301792. We thank Mary Rowland from the U.S. Forest Service providing information for a case study included in the manuscript. NR 71 TC 1 Z9 1 U1 14 U2 49 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD AUG 1 PY 2016 VL 178 BP 83 EP 91 DI 10.1016/j.jenvman.2016.02.028 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DN8FX UT WOS:000377315700009 PM 27131638 ER PT J AU Pinto, LC de Mello, CR Norton, LD Owens, PR Curi, N AF Pinto, Leandro Campos de Mello, Carlos Rogerio Norton, Lloyd Darrell Owens, Phillip Ray Curi, Nilton TI Spatial prediction of soil-water transmissivity based on fuzzy logic in a Brazilian headwater watershed SO CATENA LA English DT Article DE Atlantic Forest; Hydropedology; Mantiqueira Range; Water recharge ID LAND-USE; EXPERT KNOWLEDGE; MINAS-GERAIS; MODEL; FOREST; BASIN; CLASSIFICATION; MOUNTAINS; IMPACT; RANGE AB The Mantiqueira Range region is one of the most important Brazilian headwater regions, from which a great number of springs flow to form one of the most important watercourses for Sao Paulo, Rio de Janeiro and Minas Gerais States. These natural features make the Mantiqueira Range an important hydrological region for research and development of technologies for management and environmental preservation. Areas in which the predominant land-use is native forest have proved to be strategic for groundwater recharge purposes, and thus, to mitigate drought effects that are being observed recently in Southeastern Brazil. In this context, to identify and map these areas lead to better and more successful decision making regarding soil-water conservation and management. In headwater watersheds, the knowledge about spatial distribution of soil-water transmissivity can be very important to sustainable use and conservation of environmental resources. The aim of this study was to evaluate the application of pattern recognition on landscape classification tools combined with digital soil mapping techniques for mapping soil-water transmissivity in a watershed located at one of the most important Brazilian headwaters, in Southeastern Brazil. The model used solum depth and saturated hydraulic conductivity as soil deterministic elements and topographic indexes. The results showed that the method proposed combining terrain attributes and Geomorphons, which is a mapping tool that allows for the identification of landforms within a landscape through digital elevation model analysis, was very efficient and, therefore, used to predict the soil-water transmissivity for the watershed. Areas with high to moderate soil-water transmissivity values were associated with the steepest slopes, shallow and moderately deep Inceptisols and native forest fragments. Since the relief and solum depth features of Inceptisols did not show optimal conditions for soil water percolation, the results demonstrated that land-use and management factors are crucial for groundwater recharge in the region of the Mantiqueira Range. The results also showed that areas with native forests play a fundamental role in water distribution processes in the soil profile in mountainous catchments in Southeastern Brazil. These results strengthen the importance of soil conservation practices and adequate land-use to sustain this kind of environment in Brazil. (C) 2016 Elsevier B.V. All rights reserved. C1 [Pinto, Leandro Campos; Curi, Nilton] Univ Fed Lavras, Dept Soil Sci, POB 3037, BR-37200000 Lavras, MG, Brazil. [de Mello, Carlos Rogerio] Univ Fed Lavras, Dept Engn, POB 3037, BR-37200000 Lavras, MG, Brazil. [Norton, Lloyd Darrell] Purdue Univ, ARS, USDA, Natl Soil Eros Res Lab, 275 South Russell St, W Lafayette, IN 47906 USA. [Norton, Lloyd Darrell; Owens, Phillip Ray] Purdue Univ, Dept Agron, Lilly Hall Life Sci, 915 W State St, W Lafayette, IN 47906 USA. RP Pinto, LC (reprint author), Univ Fed Lavras, Dept Soil Sci, CP 3037, BR-37200000 Lavras, MG, Brazil. EM leandcampos@yahoo.com.br; crmello@deg.ufla.br; norton@purdue.edu; prowens@purdue.edu; niltcuri@dcs.ufla.br RI de Mello, Carlos/G-5821-2012 OI de Mello, Carlos/0000-0002-6033-5342 FU FAPEMIG [588 - CAG PPM - 00132/14, PPM VIII - 71-14]; CNPq [147446/2011-2]; Capes [014034/2013-01] FX The authors wish to thank FAPEMIG (588 - CAG PPM - 00132/14 and PPM VIII - 71-14), CNPq (147446/2011-2) and Capes (014034/2013-01) for sponsoring this research. NR 56 TC 1 Z9 1 U1 6 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD AUG PY 2016 VL 143 BP 26 EP 34 DI 10.1016/j.catena.2016.03.033 PG 9 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DN1BI UT WOS:000376800800004 ER PT J AU Liu, G Xiao, H Liu, PL Zhang, Q Zhang, JQ AF Liu, Gang Xiao, Hai Liu, Puling Zhang, Qiong Zhang, Jiaqiong TI Using rare earth elements to monitor sediment sources from a miniature model of a small watershed in the Three Gorges area of China SO CATENA LA English DT Article DE Purple soil; Soil loss; Runoff; Gully erosion; Landform ID PARTICLE-SIZE CHARACTERISTICS; SOIL-EROSION PROCESSES; SUSPENDED SEDIMENT; RIVER FLOODPLAINS; CRUST FORMATION; GULLY EROSION; LOESS PLATEAU; UPPER YANGTZE; TRACER METHOD; RILL EROSION AB Understanding soil erosion processes at different landscape positions is important in order to predict and control watershed soil losses. Rare earth elements (REEs) can be used to trace eroded soil sources but their efficacy may be soil dependent. We constructed a miniature watershed model of a small watershed located in the Three Gorges Area of China, and used oxides of eight REEs to trace the erosion of a purple soil. The miniature watershed was divided into eight regions containing a different landform type as a potential sediment source. A different REE was applied in each region. Redistributions of the REEs under three successive simulated rainfall events with intensities of 1.0, 1.5 or 2.0 mm min(-1) were examined. The percentage contribution from each region to the total soil loss from the watershed fluctuated relative to landform type during the three rainstorms. Contributions from the lower main gully decreased before stabilizing, while those from the upper main gully increased before decreasing, and those from other sources all increased before stabilizing. Overall, the contribution of the gully system, comprising main and branch gullies, was greater than that of the slopes. Contributions from the gully system tended to decrease with increases in rainfall intensity and rainstorm duration while those from the slopes increased. A comparison of the calculated and actual soil loss masses indicated that the accuracy of the REE tracing method was less for the coarse textured purple soil than those previously found for fine textured soils. The increased errors, likely due to the assumption used in the calculation that there is no particle size selectivity during erosion, needs to be addressed. This pilot study provided a technical reference for the use of REEs in monitoring sediment sources from a natural watershed, and a theoretical basis for soil conservation in the Three Gorges Area. (C) 2016 Elsevier B.V. All rights reserved. C1 [Liu, Gang; Xiao, Hai; Liu, Puling; Zhang, Qiong; Zhang, Jiaqiong] Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Peoples R China. [Liu, Gang] Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China. [Liu, Gang] Minist Water Resources, Yangling 712100, Peoples R China. [Liu, Gang] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Liu, G (reprint author), Northwest A&F Univ, Inst Soil & Water Conservat, 26 Xinong Rd, Yangling 712100, Shaanxi Provinc, Peoples R China. EM gliu@foxmail.com FU National Key Technology Research and Development Program of the Ministry of Science and Technology of China [2015BAC01B03-03]; National Natural Science Foundation of China [41201270]; West Light Foundation of the Chinese Academy of Sciences [2014-91]; Shaanxi Science and Technology Co-ordination Innovation Engineering Project [2013KTDZ03-03-01]; Scientific Research Foundation of Northwest AF University [2013BSJJ082]; Talents Research Start-up Foundation of Shaanxi Province [2013-02100-Z111021512]; China Scholarship Council [201404910141] FX We thank editors and anonymous reviewers for their valuable comments on the manuscript, and thank Dr. Glenn Wilson for his suggestions and improving the English writing of the manuscript. This research was jointly supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. 2015BAC01B03-03), the National Natural Science Foundation of China (No. 41201270), the West Light Foundation of the Chinese Academy of Sciences (No. 2014-91), the Shaanxi Science and Technology Co-ordination Innovation Engineering Project (No. 2013KTDZ03-03-01), the Scientific Research Foundation of Northwest A&F University (No. 2013BSJJ082), the Talents Research Start-up Foundation of Shaanxi Province (No. 2013-02100-Z111021512), and the China Scholarship Council (No. 201404910141). NR 55 TC 0 Z9 0 U1 4 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD AUG PY 2016 VL 143 BP 114 EP 122 DI 10.1016/j.catena.2016.03.044 PG 9 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA DN1BI UT WOS:000376800800013 ER PT J AU Diaz, JAR Kim, WH Fernandez, CP Jeong, J Afrin, F Lillehoj, HS Kim, S Kim, S Dalloul, RA Min, W AF Diaz, Joyce Anne R. Kim, Woo H. Fernandez, Cherry P. Jeong, Jipseol Afrin, Fahmida Lillehoj, Hyun S. Kim, Suk Kim, Sungwon Dalloul, Rami A. Min, Wongi TI Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Ducks; Interleukin-17D; Riemerella infection; Avian IL-17D ID IL-17 FAMILY CYTOKINES; MOLECULAR-CLONING; FUNCTIONAL-CHARACTERIZATION; INFLAMMATORY DISEASES; GENE-EXPRESSION; CHICKEN; RECEPTORS; CELLS; IMMUNITY; CDNA AB Interleukin (IL)-17D is a proinfiammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. dull-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinfiammatory cytokines such as IL-6, IL-8, and IL-1 beta in duck embryo fibroblast cells. Very low levels of dull-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The dull-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Diaz, Joyce Anne R.; Kim, Woo H.; Fernandez, Cherry P.; Afrin, Fahmida; Kim, Suk; Min, Wongi] Gyeongsang Natl Univ, Coll Vet Med, 501 Jinju Daero, Jinju 52828, Gyeongnam, South Korea. [Diaz, Joyce Anne R.; Kim, Woo H.; Fernandez, Cherry P.; Afrin, Fahmida; Kim, Suk; Min, Wongi] Gyeongsang Natl Univ, Inst Anim Med, Jinju 52828, Gyeongnam, South Korea. [Jeong, Jipseol] Natl Inst Environm Res, Environm Hlth Res Div, Inchon 22689, South Korea. [Lillehoj, Hyun S.] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Kim, Sungwon; Dalloul, Rami A.] Virginia Tech, Dept Anim & Poultry Sci, Avian Immunobiol Lab, Blacksburg, VA 24061 USA. RP Min, W (reprint author), Gyeongsang Natl Univ, Coll Vet Med, 501 Jinju Daero, Jinju 52828, Gyeongnam, South Korea. EM wongimin@gnu.ac.kr OI Min, Wongi/0000-0003-2437-7366; Dalloul, Rami/0000-0003-4690-9220 FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2015R1D1A1A02059953] FX This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A02059953). NR 40 TC 0 Z9 0 U1 2 U2 6 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD AUG PY 2016 VL 61 BP 190 EP 197 DI 10.1016/j.dci.2016.04.002 PG 8 WC Immunology; Zoology SC Immunology; Zoology GA DN1CQ UT WOS:000376804300021 PM 27060655 ER PT J AU Park, M Kim, S Fetterer, RH Dalloul, RA AF Park, Myeongseon Kim, Sungwon Fetterer, Raymond H. Dalloul, Rami A. TI Functional characterization of the turkey macrophage migration inhibitory factor SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Macrophage migration inhibitory factor; Turkey; Chemotaxis; Cytokines ID HIGH SEQUENCE IDENTITY; FACTOR MIF; IMMUNE-RESPONSE; CRYSTAL-STRUCTURE; PHENYLPYRUVATE TAUTOMERASE; SECONDARY STRUCTURE; MOLECULAR-CLONING; GENE-EXPRESSION; REGULATORY ROLE; EIMERIA-MAXIMA AB Macrophage migration inhibitory factor (MIF) is a soluble protein that inhibits the random migration of macrophages and plays a pivotal immunoregulatory function in innate and adaptive immunity. The aim of this study was to clone the turkey MIF (TkMIF) gene, express the active protein, and characterize its basic function. The full-length TkMIF gene was amplified from total RNA extracted from turkey spleen, followed by cloning into a prokaryotic (pET11a) expression vector. Sequence analysis revealed that TkMIF consists of 115 amino acids with 12.5 kDa molecular weight. Multiple sequence alignment revealed 100%, 65%, 95% and 92% identity with chicken, duck, eagle and zebra finch MIFs, respectively. Recombinant TkMIF (rTkMIF) was expressed in Escherichia coli and purified through HPLC and endotoxin removal. SDS-PAGE analysis revealed an approximately 13.5 kDa of rTkMIF monomer containing T7 tag in soluble form. Western blot analysis showed that anti-chicken MIF (ChMIF) polyclonal antisera detected a monomer form of TkMIF at approximately 13.5 kDa size. Further functional analysis revealed that rTkMIF inhibits migration of both mononuclear cells and splenocytes in a dose-dependent manner, but was abolished by the addition of anti-ChMIF polyclonal antisera. qRT-PCR analysis revealed elevated transcripts of pro inflammatory cytokines by rTkMIF in LPS-stimulated monocytes. rTkMIF also led to increased levels of IFN-gamma and IL-17F transcripts in Con A-activated splenocytes, while IL-10 and IL-13 transcripts were decreased. Overall, the sequences of both the turkey and chicken MIF have high similarity and comparable biological functions with respect to migration inhibitory activities of macrophages and enhancement of pro-inflammatory cytokine expression, suggesting that turkey and chicken MIFs would be biologically cross-reactive. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Park, Myeongseon; Kim, Sungwon; Dalloul, Rami A.] Virginia Tech, Dept Anim & Poultry Sci, Avian Immunobiol Lab, Blacksburg, VA 24061 USA. [Kim, Sungwon] Univ Edinburgh, Roslin Inst, Easter Bush EH25 9RG, Midlothian, Scotland. [Kim, Sungwon] Univ Edinburgh, R D SVS, Easter Bush EH25 9RG, Midlothian, Scotland. [Fetterer, Raymond H.] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Dalloul, RA (reprint author), Virginia Tech, Dept Anim & Poultry Sci, Avian Immunobiol Lab, Blacksburg, VA 24061 USA. EM RDalloul@vt.edu OI Dalloul, Rami/0000-0003-4690-9220 NR 46 TC 1 Z9 1 U1 2 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD AUG PY 2016 VL 61 BP 198 EP 207 DI 10.1016/j.dci.2016.04.005 PG 10 WC Immunology; Zoology SC Immunology; Zoology GA DN1CQ UT WOS:000376804300022 PM 27062968 ER PT J AU Sollmann, R White, AM Tarbill, GL Manley, PN Knapp, EE AF Sollmann, R. White, A. M. Tarbill, G. L. Manley, P. N. Knapp, E. E. TI Landscape heterogeneity compensates for fuel reduction treatment effects on Northern flying squirrel populations SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Glaucomys sabrinus; Variable thinning; Even thinning; Prescribed burn; Distribution; Sierra Nevada ID MIXED-CONIFER FORESTS; DOUGLAS-FIR FORESTS; GLAUCOMYS-SABRINUS; CAPTURE-RECAPTURE; WESTERN OREGON; SIERRA-NEVADA; UNITED-STATES; HABITAT USE; STANDS; CONSERVATION AB In the dry forests of the western United States frequent fires historically maintained a diversity of habitats in multiple seral stages. Over the past century, fire suppression and preferential harvest of large trees has led to a densification and homogenization of forests, making them more prone to larger and more severe wildfires. In response, fuel reduction treatments have become common practice in the management of dry western forests. However, the effect of fuel reduction treatments on late seral forest species, such as the Northern flying squirrels, remains a management concern. We captured and marked Northern flying squirrels within mixed conifer forest in the Stanislaus-Tuolumne Experimental Forest (California) on a continuous trapping grid (4400 traps) spanning a 120-ha study landscape in which 24 4-ha units were subject to different fuel reduction treatments (variable thin, even thin, and control, all with or without prescribed burning). The study spanned two pre-thinning and three post-thinning years. We divided the study landscape into three blocks (two with treatments, one control only). For each block we analyzed data with spatial capture-recapture models to estimate Northern flying squirrel density, and tested whether canopy closure before and after thinning and percent area burned were important predictors of density. Northern flying squirrel densities varied from 0.168 (SE 0.086) to 0.808 (SE 0.094) individuals/ha across blocks and years. Densities varied by year, independent of treatments. Percent area burned was not an important predictor of density. The effect of canopy closure was variable, but more consistently positive after thinning reduced overall canopy closure. When considered by treatment type, densities were highest in control and burn only units, and lowest in thinned units. Whereas thinning had negative effects on Northern flying squirrel density on the scale of a thinning treatment unit, our results suggest that these effects were largely absorbed by the heterogeneous landscape, as animals shifted their distribution into un-thinned areas without a decline in overall density. This highlights the need to incorporate the landscape context when evaluating the effects of forest management on wildlife. (C) 2016 Elsevier B.V. All rights reserved. C1 [Sollmann, R.; White, A. M.; Tarbill, G. L.] US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. [Sollmann, R.; Tarbill, G. L.] Oak Ridge Inst Sci & Educ, 1299 Bethel Valley Rd, Oak Ridge, TN 37830 USA. [Manley, P. N.] US Forest Serv, USDA, Pacific Southwest Res Stn, 2480 Carson Rd, Placerville, CA 95667 USA. [Knapp, E. E.] US Forest Serv, USDA, Pacific Southwest Res Stn, 3644 Avtech Pkwy, Redding, CA 96002 USA. RP Sollmann, R (reprint author), Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, 1088 Acad Surge,One Shields Ave, Davis, CA 95616 USA. EM rsollmann@ucdavis.edu; angelawhite@fs.fed.us; ginatarbill@fs.fed.us; pmanley@fs.fed.us; eknapp@fs.fed.us FU California Energy Commission [600-10-006]; USDA Forest Service Pacific Southwest Research Station FX This work would not have been possible without the contributions from an extensive group of collaborators and colleagues. In particular, Matthew Strussis-Timmer, Tray Biasiolli, Bob Carlson and Adam Rich were instrumental in leading field efforts and for data quality assurance. This research was made possible through the financial support of the California Energy Commission (contract number 600-10-006) and the USDA Forest Service Pacific Southwest Research Station. We further thank the Stanislaus National Forest for logistical support. The manuscript was greatly improved by the input of three anonymous reviewers. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 44 TC 2 Z9 2 U1 12 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 1 PY 2016 VL 373 BP 100 EP 107 DI 10.1016/j.foreco.2016.04.041 PG 8 WC Forestry SC Forestry GA DN1QB UT WOS:000376839400012 ER PT J AU Jolly, WM Hintz, J Linn, RL Kropp, RC Conrad, ET Parsons, RA Winterkamp, J AF Jolly, W. Matt Hintz, John Linn, Rodman L. Kropp, Rachael C. Conrad, Elliot. T. Parsons, Russell A. Winterkamp, Judith TI Seasonal variations in red pine (Pinus resinosa) and jack pine (Pinus banksiana) foliar physio-chemistry and their potential influence on stand-scale wildland fire behavior SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Live foliar moisture content; Spring dip; Foliar density; Flammability; Fire behavior; Fire modeling; FIRETEC ID CROWN FIRE; MOISTURE-CONTENT; FOREST; NEEDLES; FUELS; TREES AB The 'Spring Dip' in conifer live foliar moisture content (LFMC) has been well documented but the actual drivers of these variations have not been fully investigated. Here we span this knowledge gap by measuring LFMC, foliar chemistry, foliar density and foliar flammability on new and old foliage for an entire year from both Pinus resinosa (red pine) and Pinus banksiana (jack pine) at a site in Central Wisconsin. We found that needle dry mass increased by up to 70% in just three weeks and these increases were manifested as strong seasonal variations in foliar moisture content and foliar density. These needle dry mass changes were driven by an accumulation of starch in old foliage, likely resulting from springtime photosynthesis onset, and also by accumulations of sugar and crude fat in new needles as they fully matured. Foliar starch, sugar and crude fat content accounted for 84% of the variation in foliar density across both species. Flammability differences were also strongly related to changes in foliar density, where density accounted for 39% and 25% of the variations in foliar time-to-ignition of jack pine and red pine respectively. Finally, we use the computational fluid dynamics-based wildland fire model FIRETEC to examine how these foliar physio-chemical changes may influence wildland fire behavior. Under the lowest canopy density and windspeed, simulated fires in dormant condition stands did not propagate as crown fires while spring dip stands successfully spread as crown fires as a result of the higher potential energy content of the canopy. Simulated wildland fire spread rates increased by as much as 63%, nominal fireline width increased by as much as 89% and active fire area more than doubled relative to dormant season fuel conditions and the most significant changes occurred in areas with low canopy cover and low within-tree bulk density. Our results challenge the assumption that live conifer foliage flammability is limited only by its water content; this study suggests a new theory and an expanded view of the factors that dominate live fuel flammability and that subsequently influence larger scale wildland fire behavior. Published by Elsevier B.V. C1 [Jolly, W. Matt; Kropp, Rachael C.; Conrad, Elliot. T.; Parsons, Russell A.] US Forest Serv, USDA, RMRS, Fire Sci Lab, 5775 Hwy 10 W, Missoula, MT 59808 USA. [Hintz, John] Wisconsin Dept Nat Resources, 473 Griffith Ave, Wisconsin Rapids, WI 54494 USA. [Linn, Rodman L.; Winterkamp, Judith] Los Alamos Natl Lab, POB 1663, Los Alamos, NM 87544 USA. RP Jolly, WM (reprint author), US Forest Serv, USDA, RMRS, Fire Sci Lab, 5775 Hwy 10 W, Missoula, MT 59808 USA. EM mjolly@fs.fed.us; John.Hintz@wisconsin.gov; rrl@lanl.gov; rachaelckropp@fs.fed.us; eliotttconrad@fs.fed.us; rparsons@fs.fed.us; judyw@lanl.gov FU Joint Fire Science Program of the U.S. Department of Agriculture (USDA); U.S. Department of the Interior (USDI) Projects [12-1-03-30]; USDA Forest Service's Rocky Mountain Research Station (RMRS) FX This work was made possible by funding from the Joint Fire Science Program of the U.S. Department of Agriculture (USDA) and U.S. Department of the Interior (USDI) Projects (12-1-03-30), as well as by the USDA Forest Service's Rocky Mountain Research Station (RMRS). Los Alamos National Laboratory's Institutional Computing Program provided computational resources to perform the FIRETEC simulations for this work. NR 37 TC 1 Z9 1 U1 4 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 1 PY 2016 VL 373 BP 167 EP 178 DI 10.1016/j.foreco.2016.04.005 PG 12 WC Forestry SC Forestry GA DN1QB UT WOS:000376839400018 ER PT J AU Johnson, JMF Strock, JS Tallaksen, JE Reese, M AF Johnson, Jane M. F. Strock, Jeffrey S. Tallaksen, Joel E. Reese, Michael TI Corn stover harvest changes soil hydrology and soil aggregation SO SOIL & TILLAGE RESEARCH LA English DT Article DE Residue management; Cellulosic bioenergy; Soil quality ID CROP RESIDUE REMOVAL; HYDRAULIC CONDUCTIVITY; ORGANIC-MATTER; PHYSICAL-PROPERTIES; DISK PERMEAMETERS; CARBON; INFILTRATION; MANAGEMENT; ROTATION; TILLAGE AB In the United States, commercial-scale cellulosic-ethanol production using corn (Zea mays L.) stover has become a reality. As the industry matures and demand for stover increases, it is important to determine the amount of biomass that can be sustainably harvested while safe-guarding soil quality and productivity. Specific study objectives were to measure indices of soil hydrological and aggregate stability responses to harvesting stover; since stover harvest may negatively impact soil hydrological and physical properties. Responses may differ with tillage management; thus, this paper reports on two independent studies on a tilled (Chisel field) and untilled field (NT1995 field). Each field was managed in a corn/soybean (Glycine max [Merr.]) rotation and with two rates of stover return: (1) all returned (Full Return Rate) and (2) an aggressive residue harvest leaving little stover behind (Low Return Rate). Unconfined field soil hydraulic properties and soil aggregate properties were determined. Hydrological response to residue treatments in the Chisel field resulted in low water infiltration for both rates of residue removal. In NT1995 field, Full Return Rate had greater capacity to transmit water via conductive pathways, which were compromised in Low Return Rate. Collectively, indices of soil aggregation in both experiments provided evidence that the aggregates were less stable, resulting in a shift toward more small aggregates at the expense of larger aggregates when stover is not returned to the soil. In both fields, aggressive stover harvest degraded soil physical and hydrological properties. No tillage management did not protect soil in absence of adequate residue. (C) 2016 Published by Elsevier B.V. C1 [Johnson, Jane M. F.] ARS, USDA, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. [Strock, Jeffrey S.] Univ Minnesota, Southwest Res & Outreach Ctr, Lamberton, MN 56152 USA. [Tallaksen, Joel E.; Reese, Michael] Univ Minnesota, West Cent Res & Outreach Ctr, Morris, MN 56267 USA. RP Johnson, JMF (reprint author), ARS, USDA, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM jane.johnson@ars.usda.gov OI Johnson, Jane/0000-0002-1687-4007 FU USDA-Agricultural Research funding, as part of the USDA-ARS-REAP project; North Central Regional SunGrant Center at South Dakota State University by the Biomass Energy Technology Office through a USDA-NIFA Sun Grant Association [DE-FC36-05GO85041]; Renewable Development Fund FX Funding for this project provide by the USDA-Agricultural Research funding, as part of the USDA-ARS-REAP project. Additional funding the North Central Regional SunGrant Center at South Dakota State University through a grant provided by the US-DOE - Office of Biomass Products (now known as the Biomass Energy Technology Office) through a USDA-NIFA Sun Grant Association under award number DE-FC36-05GO85041 and provided by customers of Xcel Energy1 through a grant from the Renewable Development Fund. NR 51 TC 0 Z9 0 U1 11 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD AUG PY 2016 VL 161 BP 106 EP 115 DI 10.1016/j.still.2016.04.004 PG 10 WC Soil Science SC Agriculture GA DM9SG UT WOS:000376705400013 ER PT J AU Chiou, BS Valenzuela-Medina, D Bilbao-Sainz, C Klamczynski, AP Avena-Bustillos, RJ Milczarek, RR Du, WX Glenn, GM Orts, WJ AF Chiou, Bor-Sen Valenzuela-Medina, Diana Bilbao-Sainz, Cristina Klamczynski, Artur P. Avena-Bustillos, Roberto J. Milczarek, Rebecca R. Du, Wen-Xian Glenn, Greg M. Orts, William J. TI Torrefaction of almond shells: Effects of torrefaction conditions on properties of solid and condensate products SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Torrefaction; Almond shells; Condensate; Response surface methodology; Moisture sorption isotherms; Mass loss ID OIL PALM BIOMASS; AGRICULTURAL WASTES; SYNTHETIC INDICATOR; CORN STOVER; MASS-LOSS; WOOD; FUEL; GRINDABILITY; CONSTITUENTS; COMPONENTS AB Almond shells were torrefied in a fixed bed reactor and their solid and condensate products were collected for analysis. Response surface methodology was used to examine effects of torrefaction temperature (230 degrees C, 260 degrees C, and 290 degrees C) and time (60, 80, and 100 min) on mass and energy yields of solid products as well as mass yields and gross calorific values (GCVs) of condensate products. This was the first study on condensates produced during torrefaction of almond shells. Also, true density, moisture sorption isotherms, thermal stability, and elemental composition of the solid products were characterized. The mass yields of solid products drastically decreased at higher temperatures, from 85.4-92.7% at 230 degrees C to 39.4-45.3% at 290 degrees C. Also, mass yields (8.79-11.71% at 230 degrees C to 23.13-29.39% at 290 degrees C) and GCVs of condensates (2.3 +/- 0.3 to 4.3 +/- 1.0 MJ/kg at 230 degrees C to 5.6 +/- 1.0 to 7.1 +/- 1.2 MJ/kg at 290 degrees C) generally increased in value at higher temperatures and longer times. Moisture sorption isotherms of torrefied shells were measured for the first time and all torrefied shells had lower equilibrium moisture contents than raw shells. In addition, equilibrium moisture contents and GCVs of the condensates were found, for the first time, to be predicted relatively well using just the sample mass loss results. The minimum moisture contents were predicted to occur at 16.4-19.9% mass loss. Published by Elsevier B.V. C1 [Chiou, Bor-Sen; Valenzuela-Medina, Diana; Bilbao-Sainz, Cristina; Klamczynski, Artur P.; Glenn, Greg M.; Orts, William J.] USDA, Bioprod Res Unit, Albany, CA 94710 USA. [Avena-Bustillos, Roberto J.; Milczarek, Rebecca R.; Du, Wen-Xian] USDA, Hlth Proc Foods Res, Albany, CA 94710 USA. RP Chiou, BS (reprint author), USDA, Bioprod Res Unit, Albany, CA 94710 USA. EM bor-sen.chiou@ars.usda.gov FU California Department of Food and Agriculture's Specialty Crop Block Grant Program [SCB11021] FX This study was funded by the California Department of Food and Agriculture's Specialty Crop Block Grant Program (#SCB11021). NR 40 TC 2 Z9 2 U1 5 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD AUG PY 2016 VL 86 BP 40 EP 48 DI 10.1016/j.indcrop.2016.03.030 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA DN1AZ UT WOS:000376799900006 ER PT J AU Thorp, KR Wang, G Badaruddin, M Bronson, KF AF Thorp, K. R. Wang, G. Badaruddin, M. Bronson, K. F. TI Lesquerella seed yield estimation using color image segmentation to track flowering dynamics in response to variable water and nitrogen management SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Anthesis; Digital image; Fertilizer; Irrigation; Oilseed; Phenotyping ID FERTILIZATION; GROWTH; OIL; FENDLERI; ACID AB Seed oil from lesquerella (Physaria fendleri (Gray) O'Kane & Al-Shehbaz) can potentially supplement castor oil as a non-petroleum-based chemical feedstock in the production of many industrial products. However, before lesquerella will become commercially viable, further efforts are needed to address crop management challenges and to improve lesquerella varieties. Because lesquerella develops vibrant yellow flowers on top of the canopy, digital imaging can be used to track the dynamics of its indeterminate flowering period. The objective of this study was to investigate a digital image analysis approach to (1) assess differences in lesquerella flowering dynamics due to variable water and nitrogen (N) management and (2) estimate lesquerella seed yield from flowering data. During the winters of 2011-2012 and 2012-2013, field experiments tested lesquerella responses to two irrigation levels and six N fertilization rates at Maricopa, Arizona. Biomass was sampled within a 30 cm x 30 cm area twice per month, and lesquerella flowers were manually counted. Twice per week, digital images were collected with a commercial digital camera at a nadir view angle approximately 2 m above the canopy. To obtain the percentage of yellow flowers in each image, an analysis routine included (1) an image transformation to the hue, saturation, and intensity (HSI) color space and (2) a Monte Carlo approach to address uncertainty in HSI parameters used for image segmentation. The imposed irrigation and N fertilization treatments led to differences in both flower count and flower cover (p < 0.05). However, the digital imaging approach permitted more frequent measurements, which revealed fine temporal changes in flowering patterns that could be explained by management factors. Due in part to the larger sampling area for the digital imaging approach, lesquerella seed yield was better estimated using flower cover percentage (r(2) <= 0.84) from images as compared to manual flower counts (r(2) <= 0.56). Overall, the digital imaging approach provided useful information on lesquerella flowering dynamics, which was affected by water and N management and highly correlated with seed yield. Published by Elsevier B.V. C1 [Thorp, K. R.; Bronson, K. F.] USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. [Wang, G.] Bridgestone Amer Agr Operat, 4140 West Harmon Rd, Eloy, AZ 85131 USA. [Badaruddin, M.] Univ Arizona, Maricopa Agr Ctr, 37860 West Smith Enke Rd, Maricopa, AZ 85138 USA. RP Thorp, KR (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Ln, Maricopa, AZ 85138 USA. EM kelly.thorp@ars.usda.gov NR 19 TC 0 Z9 0 U1 9 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD AUG PY 2016 VL 86 BP 186 EP 195 DI 10.1016/j.indcrop.2016.03.035 PG 10 WC Agricultural Engineering; Agronomy SC Agriculture GA DN1AZ UT WOS:000376799900023 ER PT J AU Yadav, AS Saxena, GK Saxena, VK Kataria, JM Juneja, VK AF Yadav, Ajit S. Saxena, Gaurav K. Saxena, V. K. Kataria, J. M. Juneja, V. K. TI Thermal inactivation of Salmonella Typhimurium on dressed chicken skin previously exposed to acidified sodium chlorite or carvacrol SO FOOD CONTROL LA English DT Article DE Salmonella; Antimicrobial; Food safety; Stress response ID IN-GROUND CHICKEN; ENTERICA SEROVAR ENTERITIDIS; FOODBORNE PATHOGEN; ACETIC-ACID; TEMPERATURE; FOODS; CINNAMALDEHYDE; CONTAMINATION; CARCASSES; LISTERIA AB Salmonella is a leading cause of foodborne illness and live poultry is a main reservoir of this pathogen, worldwide. Cross-contamination and transportation of contaminated poultry meat act as an important vehicle of Salmonella infections in humans. In this study, we assessed the effect of two antimicrobials; acidified sodium chlorite (ASC) and carvacrol followed by thermal treatment to inactivate Salmonella Typhimurium on dressed chicken skin. D-values (time in min for the pathogen to decrease by 90%) of Salmonella Typhimurium at 56, 60 and 64 degrees C on dressed chicken skin in the control samples, determined by linear regression, were 6.17, 3.16, 1.32 min, respectively. Two D-values calculated using a logistic model, ranged from 6.28 (D-1, major population, plus T-L) and 11.66 (D-2, heat-resistant subpopulation, plus TL) min at 56 degrees C to 1.08 (D-1 plus T-L), and 2.07 (D-2 plus TL) min at 64 degrees C. Pre-dipping in 100-300 ppm ASC or 0.02-0.06% carvacrol rendered the pathogen more sensitive to the lethal effect of heat. Thus, combination of antimicrobials with thermal inactivation was more effective in reducing heat resistance of the pathogen on dressed chicken surface. The model developed will assist poultry processors in estimating the time required for specific log reductions of Salmonella Typhimurium on chicken skin and thus, will contribute in designing acceptance limits at critical control points for chicken skins at lower times and temperatures for cooking. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Yadav, Ajit S.; Saxena, Gaurav K.] ICAR Cent Avian Res Inst, Post Harvest Technol Div, Food Microbiol Lab, Izatnagar 243122, Uttar Pradesh, India. [Saxena, V. K.] ICAR Cent Avian Res Inst, Dis Genet & Biotechnol Lab, Izatnagar 243122, Uttar Pradesh, India. [Kataria, J. M.] ICAR Cent Avian Res Inst, Izatnagar 243122, Uttar Pradesh, India. [Juneja, V. K.] ARS, Residue Chem & Predict Microbiol, USDA, Wyndmoor, PA 19038 USA. RP Yadav, AS (reprint author), ICAR Cent Avian Res Inst, Post Harvest Technol Div, Food Microbiol Lab, Izatnagar 243122, Uttar Pradesh, India. EM asinghcari@gmail.com FU Department of Biotechnology (DBT), Govt. of India [BT/PR4326/AAQ/1/496/2012] FX The financial help received from Department of Biotechnology (DBT), Govt. of India (Grant No. -BT/PR4326/AAQ/1/496/2012) is duly acknowledged. NR 48 TC 1 Z9 1 U1 8 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD AUG PY 2016 VL 66 BP 227 EP 232 DI 10.1016/j.foodcont.2016.02.014 PG 6 WC Food Science & Technology SC Food Science & Technology GA DK8FX UT WOS:000375163700028 ER PT J AU Han, LJ Sapozhnikova, Y Lehotay, SJ AF Han, Lijun Sapozhnikova, Yelena Lehotay, Steven J. TI Method validation for 243 pesticides and environmental contaminants in meats and poultry by tandem mass spectrometry coupled to low-pressure gas chromatography and ultrahigh-performance liquid chromatography SO FOOD CONTROL LA English DT Article DE Pesticides; Environmental contaminants; Meat; Method validation; Analysis; LPGC-MS/MS; UHPLC-MS/MS ID SOLID-PHASE EXTRACTION; POLYBROMINATED DIPHENYL ETHERS; LC-MS/MS; RESIDUE ANALYSIS; FISH; MULTICLASS; CLEANUP; ORIGIN; FOOD; GC AB An easy and reliable high-throughput analysis method was developed and validated for 192 diverse pesticides and 51 environmental contaminants (13 PCB congeners, 14 PAHs, 7 PBDE congeners, and 17 novel flame retardants) in cattle, swine, and poultry muscle. Sample preparation was based on the "quick, easy, cheap, effective, rugged and safe" (QuEChERS) approach using filter-vial dispersive solid phase extraction (d-SPE) cleanup. Split final extracts were analyzed in parallel by low-pressure (vacuum outlet) GC-MS/MS and UHPLC-MS/MS (10 min each), providing an additional degree of confirmation for 55 overlapping LC- and GC-amenable pesticides. Analyte protectants were utilized to improve sensitivity and decrease matrix effects in GC analysis, and only filtration of initial extracts was enough to avoid ion suppression in UHPLC-MS/MS. The method was validated at three spiking levels (10, 25, and 100 ng/g) at or below established tolerance levels in the sample types. Satisfactory recoveries (70-120%) and RSDs <= 20% were achieved for 200 analytes. The validated method was successfully applied to the analysis of real-world incurred meat samples, further demonstrating the utility of the method for implementation in regulatory and commercial laboratories. Published by Elsevier Ltd. C1 [Han, Lijun; Sapozhnikova, Yelena; Lehotay, Steven J.] ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. [Han, Lijun] China Agr Univ, Coll Sci, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. RP Sapozhnikova, Y (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM Yelena.Sapozhnikova@ars.usda.gov FU China Scholarship Council FX Technical assistance of Alan Lightfield, Tawana Simons, and Robyn Moten are greatly appreciated. Lijun Han's sabbatical funding was provided by the China Scholarship Council. We thank Sam Ellis of Thomson Scientific for providing a press tray apparatus. Thanks to Catalina Yee of USDA-FSIS for providing incurred meat samples. NR 25 TC 4 Z9 4 U1 17 U2 59 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD AUG PY 2016 VL 66 BP 270 EP 282 DI 10.1016/j.foodcont.2016.02.019 PG 13 WC Food Science & Technology SC Food Science & Technology GA DK8FX UT WOS:000375163700033 ER PT J AU Trabelsi, S Lewis, MA Nelson, SO AF Trabelsi, Samir Lewis, Micah A. Nelson, Stuart O. TI Microwave moisture meter for in-shell peanut kernels SO FOOD CONTROL LA English DT Article DE Dielectric properties; Microwave moisture meter; Moisture content; Bulk density; Peanut pods; Peanut kernels ID DIELECTRIC-PROPERTIES; PARTICULATE MATERIALS; BULK-DENSITY; FREQUENCIES; SENSORS; WATER; GRAIN AB A microwave moisture meter built with off-the-shelf components was developed, calibrated and tested in the laboratory and in the field for nondestructive and instantaneous in-shell peanut kernel moisture content determination from dielectric properties measurements on unshelled peanut pod samples. The meter operates at a single frequency of 5.8 GHz and uses free-space transmission measurement principles for determining the dielectric properties of the peanut pods. From these properties, a dielectric based algorithm provides peanut kernel moisture content from measurements on pods. Field tests at a peanut buying station showed that moisture content of peanut kernels can be determined with a standard error of performance of 0.82% when compared to the official moisture meter which required the pods to be shelled before kernel moisture content can be determined. When performance of both the microwave moisture meter and the official meter were compared to the oven-drying standard method, values of the standard errors of performance were 0.53% for the microwave moisture meter and 0.87% for the official moisture meter. Published by Elsevier Ltd. C1 [Trabelsi, Samir; Lewis, Micah A.; Nelson, Stuart O.] ARS, USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. RP Trabelsi, S (reprint author), ARS, USDA, US Natl Poultry Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM Samir.trabelsi@ars.usda.gov NR 17 TC 4 Z9 4 U1 4 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD AUG PY 2016 VL 66 BP 283 EP 290 DI 10.1016/j.foodcont.2016.02.016 PG 8 WC Food Science & Technology SC Food Science & Technology GA DK8FX UT WOS:000375163700034 ER PT J AU Rice, CP McCarty, GW Bialek-Kalinski, K Zabetakis, K Torrents, A Hapeman, CJ AF Rice, Clifford P. McCarty, Gregory W. Bialek-Kalinski, Krystyna Zabetakis, Kara Torrents, Alba Hapeman, Cathleen J. TI Analysis of metolachlor ethane sulfonic acid (MESA) chirality in groundwater: A tool for dating groundwater movement in agricultural settings SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Metolachlor ethane sulfonic acid; Groundwater dating; S-metolachlor; Chiral separation; Stereoisomer fraction ID S-METOLACHLOR; RIPARIAN ZONE; TRANSPORT; FATE; SOIL; METABOLITES; WATER; RIVER; USA; MINERALIZATION AB To better address how much groundwater contributes to the loadings of pollutants from agriculture we developed a specific dating tool for groundwater residence times. This tool is based on metolachlor ethane sulfonic acid, which is a major soil metabolite of metolachlor. The chiral forms of metolachlor ethane sulfonic acid (MESA) and the chiral forms of metolachlor were examined over a 6-year period in samples of groundwater and water from a groundwater-fed stream in a riparian buffer zone. This buffer zone bordered cropland receiving annual treatments with metolachlor. Racemic (rac) metolachlor was applied for two years in the neighboring field, and subsequently S-metolachlor was used which is enriched by 88% with the S-enantiomer. Chiral analyses of the samples showed an exponential increase in abundance of the S-enantiomeric forms for MESA as a function of time for both the first order riparian buffer stream( R-2=0.80) and for groundwater within the riparian buffer ( R-2=0.96). However, the S-enrichment values for metolachlor were consistently high indicating different delivery mechanisms for MESA and metolachlor. A mean residence time of 3.8 years was determined for depletion of the initially-applied rac-metolachlor. This approach could be useful in dating groundwater and determining the effectiveness of conservation measures. One sentence summary: A mean residence time of 3.8 years was calculated for groundwater feeding a first-order stream by plotting the timed-decay for the R-enantiomer of metolachlor ethane sulfonic acid. Published by Elsevier B.V. C1 [Rice, Clifford P.; Bialek-Kalinski, Krystyna] ARS, Sustainable Agr Syst Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [McCarty, Gregory W.; Hapeman, Cathleen J.] ARS, Hydrol & Remote Sensing Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. [Zabetakis, Kara; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. RP Rice, CP (reprint author), ARS, Sustainable Agr Syst Lab, Beltsville Agr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM clifford.rice@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service FX This work was supported financially by US Department of Agriculture, Agricultural Research Service intramural projects in National Programs 211, Water Availability and Water Management, and 212, Soil and Air. NR 36 TC 0 Z9 0 U1 9 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 1 PY 2016 VL 560 BP 36 EP 43 DI 10.1016/j.scitotenv.2016.04.007 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7WI UT WOS:000375137100005 PM 27093121 ER PT J AU Zaharescu, DG Hooda, PS Burghelea, CI Polyakov, V Palanca-Soler, A AF Zaharescu, Dragos G. Hooda, Peter S. Burghelea, Carmen I. Polyakov, Viktor Palanca-Soler, Antonio TI Climate change enhances the mobilisation of naturally occurring metals in high altitude environments SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Climate change; High altitude; Trace elements; Lakebed sediment record; Weathering and transportation; Contaminant risk ID HIGH-ELEVATION SITES; TEMPERATURE-VARIATIONS; CENTRAL PYRENEES; LAKE-SEDIMENTS; LAST CENTURY; TRACE-METALS; GEOCHEMISTRY; PRECIPITATION; ACCUMULATION; RESERVOIRS AB Manmade climate change has expressed a plethora of complex effects on Earth's biogeochemical compartments. Climate change may also affect the mobilisation of natural metal sources, with potential ecological consequences beyond mountains' geographical limits; however, this question has remained largely unexplored. We investigated this by analysing a number of key climatic factors in relationship with trace metal accumulation in the sediment core of a Pyrenean lake. The sediment metal contents showed increasing accumulation trend over time, and their levels varied in step with recent climate change. The findings further revealed that a rise in the elevation of freezing level, a general increase in the frequency of drier periods, changes in the frequency of winter freezing days and a reducing snow cover since the early 1980s, together are responsible for the observed variability and augmented accumulation of trace metals. Our results provide clear evidence of increased mobilisation of natural metal sources - an overlooked effect of climate change on the environment. With further alterations in climate equilibrium predicted over the ensuing decades, it is likely that mountain catchments in metamorphic areas may become significant sources of trace metals, with potentially harmful consequences for the wider environment. (C) 2016 Elsevier B.V. All rights reserved. C1 [Zaharescu, Dragos G.; Burghelea, Carmen I.] Univ Arizona, Biosphere 2, Tucson, AZ USA. [Zaharescu, Dragos G.; Burghelea, Carmen I.; Palanca-Soler, Antonio] Univ Vigo, Fac Biol Sci, Vigo 36310, Spain. [Zaharescu, Dragos G.; Hooda, Peter S.] Kingston Univ London, Sch Nat & Built Environm, London, ON, Canada. [Polyakov, Viktor] USDA, Southwest Res Ctr, Tucson, AZ USA. RP Zaharescu, DG (reprint author), Univ Arizona, Biosphere 2, Tucson, AZ USA.; Zaharescu, DG (reprint author), Univ Vigo, Fac Biol Sci, Vigo 36310, Spain.; Zaharescu, DG (reprint author), Kingston Univ London, Sch Nat & Built Environm, London, ON, Canada. EM zaha_dragos@yahoo.com; p.hooda@kingston.ac.uk; bcarmen@email.arizona.edu; viktor.polyakov@ars.usda.gov; apalanca@uvigo.es RI Palanca Soler, Antonio/B-4206-2010; OI zaharescu, dragos/0000-0002-6593-3739 FU Felix de Azara award of Huesca Provincial Council, Spain; University of Vigo, Spain; Kingston University London, UK FX This work was financially supported by a Felix de Azara award of Huesca Provincial Council, Spain, as well as by University of Vigo, Spain and Kingston University London, UK. The sponsors were not involved in the research or article preparation. We are deeply grateful to the Spanish National Institute of Meteorology in Madrid, Spain for providing climate data, to Mariano Lastra-Valdor, from the University of Vigo, for his substantial support during project execution, as well as to Craig Rasmussen, Joel Cuello and Cody Brown from the University of Arizona for their help with particle size analysis. NR 56 TC 1 Z9 1 U1 9 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 1 PY 2016 VL 560 BP 73 EP 81 DI 10.1016/j.scitotenv.2016.04.002 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7WI UT WOS:000375137100009 PM 27093125 ER PT J AU Hohweyer, J Cazeaux, C Travaille, E Languet, E Dumetre, A Aubert, D Terryn, C Dubey, JP Azas, N Houssin, M Loic, F Villena, I La Carbona, S AF Hohweyer, Jeanne Cazeaux, Catherine Travaille, Emmanuelle Languet, Emilie Dumetre, Aurelien Aubert, Dominique Terryn, Christine Dubey, Jitender P. Azas, Nadine Houssin, Maryline Loic, Favennec Villena, Isabelle La Carbona, Stephanie TI Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves SO FOOD MICROBIOLOGY LA English DT Article DE Protozoa; Immunomagnetic separation; Microscopy; Real-time qPCR; Vegetables; Fruits; Persistence; Viability ID MEDIATED ISOTHERMAL AMPLIFICATION; POLYMERASE-CHAIN-REACTION; GONDII OOCYSTS; IMMUNOMAGNETIC SEPARATION; PARVUM OOCYSTS; CYCLOSPORA-CAYETANENSIS; WATERBORNE TRANSMISSION; INTESTINALIS CYSTS; STANDARD METHODS; SALAD PRODUCTS AB Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD = 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD = 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD <1 to 3 (oo)cysts/g and RR between 2 and 35%. This procedure was coupled to RT-qPCR analyses and showed that the three protozoa persisted on the leaves of basil and remained viable following storage at 4 degrees C for 8 days. These data strengthen the need to consider these protozoa in food safety. (C) 2016 Elsevier Ltd. All rights reserved. C1 [Hohweyer, Jeanne; Aubert, Dominique; Villena, Isabelle] Univ Reims, Fac Med, Protozooses Transmises Alimentat, EA 3800,Lab Parasitol Mycol, SFR Cap Sante Fed 4231,51 Rue Cognacq Jay, F-51096 Reims, France. [Cazeaux, Catherine; Languet, Emilie; La Carbona, Stephanie] ACTALIA Food Safety Dept, 310 Rue Popielujko, F-50000 St Lo, France. [Travaille, Emmanuelle; Houssin, Maryline] Lab Frank Duncombe, 1 Route Rosel, F-14053 Caen 4, France. [Dumetre, Aurelien; Azas, Nadine] Aix Marseille Univ, Fac Pharm, UMR MD3, Infect Parasitaires Transmiss Physiopathol & Ther, 27 Blvd Jean Moulin,CS 30064, F-13385 Marseille 05, France. [Terryn, Christine] Univ Reims, Plateforme Imagerie Cellulaire & Tissulaire, 51 Rue Cognacq Jay, F-51095 Reims, France. [Dubey, Jitender P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. [Loic, Favennec] Univ Rouen, EA 3800, Protozooses Transmises Alimentat Lab Parasitol My, F-76183 Rouen, France. RP La Carbona, S (reprint author), ACTALIA Food Safety Dept, 310 Rue Popielujko, F-50000 St Lo, France. EM jeanne.hohweyer@gmail.com; s.lacarbona@actalia.eu RI Dumetre, Aurelien/N-9682-2016 OI Dumetre, Aurelien/0000-0001-5494-6326 FU French National Research Agency [ANR 09 ALIA 009]; Aix Marseille University (Preciput); region council of Basse Normandie; ANR; TERRALIA, a French organization FX This work was financially supported by the French National Research Agency (grant ANR 09 ALIA 009), Aix Marseille University (Preciput 2011) and the region council of Basse Normandie. J. Hohweyer was supported by an ANR PhD fellowship. We thank TERRALIA, a French organization that promotes the food industry, for supporting this project. NR 75 TC 0 Z9 0 U1 8 U2 43 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD AUG PY 2016 VL 57 BP 36 EP 44 DI 10.1016/j.fm.2016.01.002 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA DI5XF UT WOS:000373573100006 PM 27052700 ER PT J AU Carter, MQ Louie, JW Feng, D Zhong, W Brandl, MT AF Carter, Michelle Qiu Louie, Jacqueline W. Feng, Doris Zhong, Wayne Brandl, Maria T. TI Curli fimbriae are conditionally required in Escherichia coli O157:H7 for initial attachment and biofilm formation SO FOOD MICROBIOLOGY LA English DT Article DE Escherichia coli O157:H7; Foodborne pathogen; Curli fimbriae; Attachment; Biofilm; Spinach; Plant; Produce; Stainless steel; Glass; Surface ID ENTERICA SEROVAR THOMPSON; SALMONELLA-ENTERICA; STAINLESS-STEEL; LISTERIA-MONOCYTOGENES; BACTERIAL BIOFILMS; SURFACE-STRUCTURES; SPINACH LEAVES; LETTUCE LEAVES; CSGD PROMOTER; O157-H7 AB Several species of enteric pathogens produce curli fimbriae, which may affect their interaction with surfaces and other microbes in nonhost environments. Here we used two Escherichia coli O157:H7 outbreak strains with distinct genotypes to understand the role of curli in surface attachment and biofilm formation in several systems relevant to fresh produce production and processing. Curli significantly enhanced the initial attachment of E. coli O157:H7 to spinach leaves and stainless steel surfaces by 5-fold. Curli was also required for E. coli O157:H7 biofilm formation on stainless steel and enhanced biofilm production on glass by 19-27 fold in LB no-salt broth. However, this contribution was not observed when cells were grown in sterile spinach lysates. Furthermore, both strains of E. coli O157:H7 produced minimal biofilms on polypropylene in LB no-salt broth but considerable amounts in spinach lysates. Under the latter conditions, curli appeared to slightly increase biofilm production. Importantly, curli played an essential role in the formation of mixed biofilm by E. coli O157:H7 and plant-associated microorganisms in spinach leaf washes, as revealed by confocal microscopy. Little or no E. coli O157:H7 biofilms were detected at 4 degrees C, supporting the importance of temperature control in postharvest and produce processing environments. Published by Elsevier Ltd. C1 [Carter, Michelle Qiu; Louie, Jacqueline W.; Feng, Doris; Zhong, Wayne; Brandl, Maria T.] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA USA. RP Carter, MQ (reprint author), Western Reg Res Ctr, Produce Safety & Microbiol Unit, 800 Buchanan St, Albany, CA 94710 USA. EM michelle.carter@ars.usda.gov FU USDA-ARS CRIS project [5325-42000-046-00D] FX We wish to thank Anne Bates for assistance with confocal microscopy and Yaguang Zhou for assistance with construction of gfp-expressing E. coli O157:H7. This work was supported by USDA-ARS CRIS project 5325-42000-046-00D. NR 58 TC 1 Z9 1 U1 9 U2 79 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0740-0020 EI 1095-9998 J9 FOOD MICROBIOL JI Food Microbiol. PD AUG PY 2016 VL 57 BP 81 EP 89 DI 10.1016/j.fm.2016.01.006 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology SC Biotechnology & Applied Microbiology; Food Science & Technology; Microbiology GA DI5XF UT WOS:000373573100011 PM 27052705 ER PT J AU Harrison, RL Rowley, DL Funk, CJ AF Harrison, Robert L. Rowley, Daniel L. Funk, C. Joel TI The Complete Genome Sequence of Plodia Interpunctella Granulovirus: Evidence for Horizontal Gene Transfer and Discovery of an Unusual Inhibitor-of-Apoptosis Gene SO PLOS ONE LA English DT Article ID INDIAN MEAL MOTH; GRANULOSIS-VIRUS; BOMBYX-MORI; FAMILY BACULOVIRIDAE; HELICOVERPA-ARMIGERA; HUBNER LEPIDOPTERA; LYMANTRIA-DISPAR; DNA-REPLICATION; BASIC-PROTEIN; INSECT AB The Indianmeal moth, Plodia interpunctella (Lepidoptera: Pyralidae), is a common pest of stored goods with a worldwide distribution. The complete genome sequence for a larval pathogen of this moth, the baculovirus Plodia interpunctella granulovirus (PiGV), was determined by next-generation sequencing. The PiGV genome was found to be 112, 536 bp in length with a 44.2% G+C nucleotide distribution. A total of 123 open reading frames (ORFs) and seven homologous regions (hrs) were identified and annotated. Phylogenetic inference using concatenated alignments of 36 baculovirus core genes placed PiGV in the "b" clade of viruses from genus Betabaculovirus with a branch length suggesting that PiGV represents a distinct betabaculovirus species. In addition to the baculovirus core genes and orthologues of other genes found in other betabaculovirus genomes, the PiGV genome sequence contained orthologues of the bidensovirus NS3 gene, as well as ORFs that occur in alphabaculoviruses but not betabaculoviruses. While PiGV contained an orthologue of inhibitor of apoptosis-5 (iap-5), an orthologue of inhibitor of apoptosis-3 (iap-3) was not present. Instead, the PiGV sequence contained an ORF (PiGV ORF81) encoding an IAP homologue with sequence similarity to insect cellular IAPs, but not to viral IAPs. Phylogenetic analysis of baculovirus and insect IAP amino acid sequences suggested that the baculovirus IAP-3 genes and the PiGV ORF81 IAP homologue represent different lineages arising from more than one acquisition event. The presence of genes from other sources in the PiGV genome highlights the extent to which baculovirus gene content is shaped by horizontal gene transfer. C1 [Harrison, Robert L.; Rowley, Daniel L.] USDA ARS, Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Funk, C. Joel] John Brown Univ, Dept Biol, Siloam Springs, AR USA. RP Harrison, RL (reprint author), USDA ARS, Beltsville Agr Res Ctr, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. EM Robert.L.Harrison@ars.usda.gov OI Harrison, Robert/0000-0002-8348-3874 FU National Institute of General Medical Sciences [P20 GM103429]; Arkansas INBRE program; National Institute of General Medical Sciences, (NIGMS) [P20 GM103429] FX This work was supported by funding from the National Institute of General Medical Sciences (https://www.nigms.nih.gov), grant number P20 GM103429, awarded to CJF. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; The authors would like to thank Jeff Lord (USDA-ARS) for providing a sample of PiGV and William McGaughey and Lee Bulla for information on the source of the PiGV isolate sequenced in this study. Equipment used for purification of the virus and viral DNA isolation was made possible from support by the Arkansas INBRE program, with a grant from the National Institute of General Medical Sciences, (NIGMS), P20 GM103429. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 78 TC 1 Z9 1 U1 2 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 29 PY 2016 VL 11 IS 7 AR e0160389 DI 10.1371/journal.pone.0160389 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IM UT WOS:000381516300047 PM 27472489 ER PT J AU Shi, YY Thomasson, JA Murray, SC Pugh, NA Rooney, WL Shafian, S Rajan, N Rouze, G Morgan, CLS Neely, HL Rana, A Bagavathiannan, MV Henrickson, J Bowden, E Valasek, J Olsenholler, J Bishop, MP Sheridan, R Putman, EB Popescu, S Burks, T Cope, D Ibrahim, A McCutchen, BF Baltensperger, DD Avant, RV Vidrine, M Yang, CH AF Shi, Yeyin Thomasson, J. Alex Murray, Seth C. Pugh, N. Ace Rooney, William L. Shafian, Sanaz Rajan, Nithya Rouze, Gregory Morgan, Cristine L. S. Neely, Haly L. Rana, Aman Bagavathiannan, Muthu V. Henrickson, James Bowden, Ezekiel Valasek, John Olsenholler, Jeff Bishop, Michael P. Sheridan, Ryan Putman, Eric B. Popescu, Sorin Burks, Travis Cope, Dale Ibrahim, Amir McCutchen, Billy F. Baltensperger, David D. Avant, Robert V., Jr. Vidrine, Misty Yang, Chenghai TI Unmanned Aerial Vehicles for High-Throughput Phenotyping and Agronomic Research SO PLOS ONE LA English DT Article ID PRECISION AGRICULTURE; VEGETATION INDEX; GRAIN-YIELD; UAV IMAGES; CORN; ENVIRONMENT; IMPROVEMENT; BIOMASS; SYSTEMS; WHEAT AB Advances in automation and data science have led agriculturists to seek real-time, high-quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural data collection systems. Using UAVs for agricultural remote sensing is an innovative technology that differs from traditional remote sensing in more ways than strictly higher-resolution images; it provides many new and unique possibilities, as well as new and unique challenges. Herein we report on processes and lessons learned from year 1-the summer 2015 and winter 2016 growing seasons-of a large multidisciplinary project evaluating UAV images across a range of breeding and agronomic research trials on a large research farm. Included are team and project planning, UAV and sensor selection and integration, and data collection and analysis workflow. The study involved many crops and both breeding plots and agronomic fields. The project's goal was to develop methods for UAVs to collect high-quality, high-volume crop data with fast turnaround time to field scientists. The project included five teams: Administration, Flight Operations, Sensors, Data Management, and Field Research. Four case studies involving multiple crops in breeding and agronomic applications add practical descriptive detail. Lessons learned include critical information on sensors, air vehicles, and configuration parameters for both. As the first and most comprehensive project of its kind to date, these lessons are particularly salient to researchers embarking on agricultural research with UAVs. C1 [Shi, Yeyin; Thomasson, J. Alex] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Murray, Seth C.; Pugh, N. Ace; Rooney, William L.; Shafian, Sanaz; Rajan, Nithya; Rouze, Gregory; Morgan, Cristine L. S.; Neely, Haly L.; Rana, Aman; Bagavathiannan, Muthu V.; Ibrahim, Amir; Baltensperger, David D.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Henrickson, James; Bowden, Ezekiel; Valasek, John] Texas A&M Univ, Dept Aerosp Engn, College Stn, TX 77843 USA. [Olsenholler, Jeff; Bishop, Michael P.] Texas A&M Univ, Dept Geog, College Stn, TX 77843 USA. [Sheridan, Ryan; Putman, Eric B.; Popescu, Sorin] Texas A&M Univ, Dept Ecosyst Sci & Management, LASERS Lab, College Stn, TX 77843 USA. [Burks, Travis; Cope, Dale] Texas A&M Univ, Dept Mech Engn, College Stn, TX 77843 USA. [Shi, Yeyin; Thomasson, J. Alex; Murray, Seth C.; Rooney, William L.; Shafian, Sanaz; Rajan, Nithya; Morgan, Cristine L. S.; Neely, Haly L.; Rana, Aman; Bagavathiannan, Muthu V.; Popescu, Sorin; Ibrahim, Amir; McCutchen, Billy F.; Baltensperger, David D.; Avant, Robert V., Jr.; Vidrine, Misty] Texas A&M Univ, Texas A&M AgriLife Res, College Stn, TX 77843 USA. [Yang, Chenghai] USDA ARS, Aerial Applicat Technol Res Unit, 3103 F&B Rd, College Stn, TX 77845 USA. RP Shi, YY (reprint author), Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA.; Shi, YY (reprint author), Texas A&M Univ, Texas A&M AgriLife Res, College Stn, TX 77843 USA. EM yeyin.shi@tamu.edu RI Popescu, Sorin/D-5981-2015 OI Popescu, Sorin/0000-0002-8155-8801 FU Texas A&M AgriLife Research; Texas Engineering Experiment Station; Texas A&M Center for Geospatial Sciences, Applications and Technology (GEOSAT); Texas A&M Center for Autonomous Vehicles and Sensor Systems (CANVASS); USDA Hatch funds FX This project was supported by Texas A&M AgriLife Research, the Texas Engineering Experiment Station, Texas A&M Center for Geospatial Sciences, Applications and Technology (GEOSAT), and Texas A&M Center for Autonomous Vehicles and Sensor Systems (CANVASS). Field research projects were supported by USDA Hatch funds and other funding to individual investigators. NR 46 TC 3 Z9 3 U1 44 U2 45 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 29 PY 2016 VL 11 IS 7 AR e0159781 DI 10.1371/journal.pone.0159781 PG 26 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IM UT WOS:000381516300020 PM 27472222 ER PT J AU Duque, H Naranjo, J Carrillo, C Burbano, A Vargas, J Pauszek, L Olesen, I Sanchez-Vazquez, MJ Cosivi, O Allende, RM AF Duque, Hernando Naranjo, Jose Carrillo, Consuelo Burbano, Alexandra Vargas, Javier Pauszek, Lisa Olesen, Ian Sanchez-Vazquez, Manuel J. Cosivi, Ottorino Allende, Rossana M. TI Protection induced by a commercial bivalent vaccine against Foot-and-Mouth Disease 2010 field virus from Ecuador SO VACCINE LA English DT Article DE Foot-and-Mouth Disease; Vaccine matching; Expectancy of protection; Vaccination; Disease control ID CROSS-PROTECTION; SEROLOGY; STRAIN AB Foot-and-Mouth Disease serotype O circulated endemically in Ecuador for many years, with an upsurge occurring in 2009. This manuscript describes retrospectively in vitro and in vivo laboratory studies to predict the field effectiveness of a commercial FMD vaccine to protect against the field strain, and explains the key actions and epidemiological strategies followed by the country to control the disease. The results established that the use of a good quality oil vaccine, manufactured with strains that were isolated long ago: O1 Campos Br/58 and A24 Cruzeiro Br/55; combined with the correct epidemiological strategies, are useful to control field strains when used in periodic biannual vaccination campaigns. Published by Elsevier Ltd. C1 [Duque, Hernando; Carrillo, Consuelo; Pauszek, Lisa; Olesen, Ian] USDA, APHIS, PIADC, FADDL, Greenport, NY USA. [Burbano, Alexandra; Vargas, Javier] Agencia Ecuatoriana Aseguramiento Calidad Agro AG, Av Interocean Km 14 1-2, Tumbaco, Ecuador. [Naranjo, Jose; Sanchez-Vazquez, Manuel J.; Cosivi, Ottorino; Allende, Rossana M.] PANAFTOSA PAHO, Pan Amer Foot & Mouth Dis Ctr, Av Governador Leonel Moura Brizola 7778, BR-25045002 Duque De Caxias, RJ, Brazil. RP Allende, RM (reprint author), WHO, PANAFTOSA PAHO, Pan Amer Foot & Mouth Dis Ctr, Av Governador Leonel Moura Brizola 7778, BR-25045002 Duque De Caxias, RJ, Brazil. EM rallende@paho.org FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-08-X-00500] FX We are grateful to VECOL for providing the vaccine, and to the staff of the animal research branch of Plum Animal Disease Center. The vaccination/challenge cross protection project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-08-X-00500. The field activities in Ecuador were partially founded by interinstitutional agreement with CNPC/Brasil and PANAFTOSA-PAHO/WHO. Authors also thank staff of ACONASA/Paraguay, MGAP/Uruguay, MAPA/Brasil and SENACSA/Paraguay for providing technical cooperation in field activities. We are thankful to Mrs. Astrid da Rocha Pimentel for carefully reviewing the references section of the manuscript. NR 18 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUL 29 PY 2016 VL 34 IS 35 BP 4140 EP 4144 DI 10.1016/j.vaccine.2016.06.073 PG 5 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DT0JR UT WOS:000381170300008 PM 27395565 ER PT J AU Winkler, LR Bonman, JM Chao, SAM AdmassuYimer, B Bockelman, H Klos, KE AF Winkler, Louisa R. Bonman, J. Michael Chao, Shiaoman AdmassuYimer, B. Bockelman, Harold Klos, Kathy Esvelt TI Population Structure and Genotype Phenotype Associations in a Collection of Oat Landraces and Historic Cultivars SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE historic oat germplasm collection; association mapping; population structure; genetic diversity; lemma color; oat breeding history ID QUANTITATIVE TRAIT LOCI; YELLOW DWARF VIRUS; AVENA-SATIVA L.; GENOME-WIDE ASSOCIATION; RUST RESISTANCE GENES; HEXAPLOID OAT; CROWN RUST; LINKAGE DISEQUILIBRIUM; OIL CONTENT; MOLECULAR MARKERS AB Population structure and genetic architecture of phenotypic traits in oat (Avena sativa L) remain relatively under-researched compared to other small grain species. This study explores the historic context of current elite germplasm, including phenotypic and genetic characterization, with a particular focus on identifying under-utilized areas. A diverse panel of cultivated oat accessions was assembled from the USDA National Small Grains Collection to represent a gene pool relatively unaffected by twentieth century breeding activity and unlikely to have been included in recent molecular studies. The panel was genotyped using an oat iSelect 6K beadchip SNP array. The final dataset included 759 unique individuals and 2,715 polymorphic markers. Some population structure was apparent, with the first three principal components accounting for 38.8% of variation and 73% of individuals belonging to one of three clusters. One cluster with high genetic distinctness appears to have been largely overlooked in twentieth century breeding. Classification and phenotype data provided by the Germplasm Resources Information Network were evaluated for their relationship to population structure. Of the structuring variables evaluated, improvement status (cultivar or landrace) was relatively unimportant, indicating that landraces and cultivars included in the panel were all sampled from a similar underlying population. Instead, lemma color and region of origin showed the strongest explanatory power. An exploratory association mapping study of the panel using a subset of 2,588 mapped markers generated novel indications of genomic regions associated with awn frequency, kernels per spikelet, lemma color, and panicle type. Further results supported previous findings of loci associated with barley yellow dwarf virus tolerance, crown rust (caused by Puccinia coronata f. sp. avenae) resistance, days to anthesis, and growth habit (winter/spring). In addition, two novel loci were identified for crown rust resistance. C1 [Winkler, Louisa R.] Washington State Univ, Dept Crop & Soil Sci, Sustainable Seed Syst Lab, Pullman, WA 99164 USA. [Bonman, J. Michael; AdmassuYimer, B.; Bockelman, Harold; Klos, Kathy Esvelt] USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Chao, Shiaoman] USDA ARS, Cereal Crops Res Unit, Fargo, ND USA. RP Klos, KE (reprint author), USDA ARS, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. EM kathy.klos@ars.usda.gov FU US Department of Agriculture, Agriculture Research Service FX This work was funded by the US Department of Agriculture, Agriculture Research Service; and by a graduate research assistantship from the Seed Matters initiative of Clif Bar Family Foundation. NR 70 TC 2 Z9 2 U1 11 U2 13 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUL 29 PY 2016 VL 7 AR 1077 DI 10.3389/fpls.2016.01077 PG 15 WC Plant Sciences SC Plant Sciences GA DS6JP UT WOS:000380888400001 PM 27524988 ER PT J AU Salehi, S Howe, K Brooks, J Lawrence, ML Bailey, RH Karsi, A AF Salehi, Sanaz Howe, Kevin Brooks, John Lawrence, Mark L. Bailey, R. Hartford Karsi, Attila TI Identification of Salmonella enterica serovar Kentucky genes involved in attachment to chicken skin SO BMC MICROBIOLOGY LA English DT Article DE Salmonella; Kentucky; Flagella; Poultry; Skin; Attachment ID POULTRY SKIN; PSEUDOMONAS-AERUGINOSA; TYPHIMURIUM; TRANSPOSON; ADHESION; SURFACE; POPULATION; SEROTYPES; STRAINS AB Background: Regardless of sanitation practices implemented to reduce Salmonella prevalence in poultry processing plants, the problem continues to be an issue. To gain an understanding of the attachment mechanism of Salmonella to broiler skin, a bioluminescent-based mutant screening assay was used. A random mutant library of a field-isolated bioluminescent strain of Salmonella enterica serovar Kentucky was constructed. Mutants' attachment to chicken skin was assessed in 96-well plates containing uniform 6 mm diameter pieces of circular chicken skin. After washing steps, mutants with reduced attachment were selected based on reduced bioluminescence, and transposon insertion sites were identified. Results: Attachment attenuation was detected in transposon mutants with insertion in genes encoding flagella biosynthesis, lipopolysaccharide core biosynthesis protein, tryptophan biosynthesis, amino acid catabolism pathway, shikimate pathway, tricarboxylic acid (TCA) cycle, conjugative transfer system, multidrug resistant protein, and ATP-binding cassette (ABC) transporter system. In particular, mutations in S. Kentucky flagellar biosynthesis genes (flgA, flgC, flgK, flhB, and flgJ) led to the poorest attachment of the bacterium to skin. Conclusions: The current study indicates that attachment of Salmonella to broiler skin is a multifactorial process, in which flagella play an important role. C1 [Salehi, Sanaz; Howe, Kevin; Bailey, R. Hartford] Mississippi State Univ, Coll Vet Med, Dept Pathobiol & Populat Med, Mississippi State, MS 39762 USA. [Brooks, John] USDA ARS, Genet & Precis Agr Unit, Mississippi State, MS USA. [Lawrence, Mark L.; Karsi, Attila] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. RP Bailey, RH (reprint author), Mississippi State Univ, Coll Vet Med, Dept Pathobiol & Populat Med, Mississippi State, MS 39762 USA.; Karsi, A (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM rhbailey@cvm.msstate.edu; karsi@cvm.msstate.edu FU USDA ARS Agreement [58-6402-2729]; USDA CRIS project, "Mississippi Center for Food Safety and Post-Harvest Technology" [MIS501170] FX This project was funded by USDA ARS Agreement #58-6402-2729, which is operated under USDA CRIS project MIS501170, "Mississippi Center for Food Safety and Post-Harvest Technology." NR 24 TC 1 Z9 1 U1 2 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD JUL 29 PY 2016 VL 16 AR 168 DI 10.1186/s12866-016-0781-9 PG 7 WC Microbiology SC Microbiology GA DS4WW UT WOS:000380783300001 PM 27473153 ER PT J AU Reinhart, KO Vermeire, LT AF Reinhart, Kurt O. Vermeire, Lance T. TI Soil Aggregate Stability and Grassland Productivity Associations in a Northern Mixed-Grass Prairie SO PLOS ONE LA English DT Article ID ARBUSCULAR MYCORRHIZAL FUNGI; SPATIAL AUTOCORRELATION; ORGANIC-MATTER; GREAT-PLAINS; PLANT; CARBON; SITES; STABILIZATION; DEGRADATION; INDICATORS AB Soil aggregate stability data are often predicted to be positively associated with measures of plant productivity, rangeland health, and ecosystem functioning. Here we revisit the hypothesis that soil aggregate stability is positively associated with plant productivity. We measured local (plot-to-plot) variation in grassland community composition, plant (above-ground) biomass, root biomass,% water-stable soil aggregates, and topography. After accounting for spatial autocorrelation, we observed a negative association between % water-stable soil aggregates (0.25-1 and 1-2 mm size classes of macroaggregates) and dominant graminoid biomass, and negative associations between the % water-stable aggregates and the root biomass of a dominant sedge (Carex filifolia). However, variation in total root biomass (0-10 or 0-30 cm depths) was either negatively or not appreciably associated with soil aggregate stabilities. Overall, regression slope coefficients were consistently negative thereby indicating the general absence of a positive association between measures of plant productivity and soil aggregate stability for the study area. The predicted positive association between factors was likely confounded by variation in plant species composition. Specifically, sampling spanned a local gradient in plant community composition which was likely driven by niche partitioning along a subtle gradient in elevation. Our results suggest an apparent trade-off between some measures of plant biomass production and soil aggregate stability, both known to affect the land's capacity to resist erosion. These findings further highlight the uncertainty of plant biomass-soil stability associations. C1 [Reinhart, Kurt O.; Vermeire, Lance T.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Reinhart, KO (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM kurt.reinhart@ars.usda.gov FU United States Department of Agriculture appropriated funds (Current Research Information System) [5434-21630-003-00D] FX This work was funded by United States Department of Agriculture appropriated funds (Current Research Information System #5434-21630-003-00D). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 51 TC 0 Z9 0 U1 16 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 28 PY 2016 VL 11 IS 7 AR e0160262 DI 10.1371/journal.pone.0160262 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IL UT WOS:000381516100140 PM 27467598 ER PT J AU Ellison, MA McMahon, MB Bonde, MR Palmer, CL Luster, DG AF Ellison, Mitchell A. McMahon, Michael B. Bonde, Morris R. Palmer, Cristi L. Luster, Douglas G. TI In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections SO PLANT METHODS LA English DT Article DE Basidiomycota; Pucciniomycotina; Rust fungus; In situ hybridization; Puccinia horiana; Uromyces transversalis; Phakopsora pachyrhizi; Chrysanthemum x morifolium; Gladiolus x hortulanus; Glycine max ID ACTIN GENE-EXPRESSION; PROBES; IDENTIFICATION; VISUALIZATION; SEQUENCES; MICROORGANISMS; PATHOLOGY; FISH; MAP AB Background: Rust fungi are obligate pathogens with multiple life stages often including different spore types and multiple plant hosts. While individual rust pathogens are often associated with specific plants, a wide range of plant species are infected with rust fungi. To study the interactions between these important pathogenic fungi and their host plants, one must be able to differentiate fungal tissue from plant tissue. This can be accomplished using the In situ hybridization (ISH) protocol described here. Results: To validate reproducibility using the ISH protocol, samples of Chrysanthemum x morifolium infected with Puccinia horiana, Gladiolus x hortulanus infected with Uromyces transversalis and Glycine max infected with Phakopsora pachyrhizi were tested alongside uninfected leaf tissue samples. The results of these tests show that this technique clearly distinguishes between rust pathogens and their respective host plant tissues. Conclusions: This ISH protocol is applicable to rust fungi and potentially other plant pathogenic fungi as well. It has been shown here that this protocol can be applied to pathogens from different genera of rust fungi with no background staining of plant tissue. We encourage the use of this protocol for the study of plant pathogenic fungi in paraffin embedded sections of host plant tissue. C1 [McMahon, Michael B.; Bonde, Morris R.; Luster, Douglas G.] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Ellison, Mitchell A.] Univ Pittsburgh, Sch Med, Dept Biol, Pittsburgh, PA USA. [Palmer, Cristi L.] Rutgers State Univ, IR Project 4, Princeton, NJ USA. RP Luster, DG (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. EM doug.luster@ars.usda.gov FU USDA, ARS; USDA APHIS Farm Bill research grants FX This work was supported by USDA, ARS and by USDA APHIS Farm Bill 10007 research grants coordinated by author Dr. Cristi L. Palmer, IR-4 Project, Rutgers University. NR 37 TC 0 Z9 0 U1 5 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-4811 J9 PLANT METHODS JI Plant Methods PD JUL 27 PY 2016 VL 12 AR 37 DI 10.1186/s13007-016-0137-3 PG 11 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA DU6VW UT WOS:000382353700001 PM 27471544 ER PT J AU Makkar, SK Rath, NC Packialakshmi, B Zhou, ZY Huff, GR Donoghue, AM AF Makkar, S. K. Rath, N. C. Packialakshmi, B. Zhou, Z. Y. Huff, G. R. Donoghue, A. M. TI Nutritional Supplement of Hatchery Eggshell Membrane Improves Poultry Performance and Provides Resistance against Endotoxin Stress SO PLOS ONE LA English DT Article ID BROILER-CHICKENS; IMMUNE-RESPONSE; GENE-EXPRESSION; INFLAMMATORY CYTOKINES; LIPOPOLYSACCHARIDE; ANTIBODY; INTERLEUKIN-10; MILK; ALTERNATIVES; ANTIBIOTICS AB Eggshells are significant part of hatchery waste which consist of calcium carbonate crust, membranes, and proteins and peptides of embryonic origins along with other entrapped contaminants including microbes. We hypothesized that using this product as a nutritional additive in poultry diet may confer better immunity to the chickens in the paradigm of mammalian milk that enhances immunity. Therefore, we investigated the effect of hatchery eggshell membranes (HESM) as a short term feed supplement on growth performance and immunity of chickens under bacterial lipopolysaccharide (LPS) challenged condition. Three studies were conducted to find the effect of HESM supplement on post hatch chickens. In the first study, the chickens were fed either a control diet or diets containing 0.5% whey protein or HESM as supplement and evaluated at 5 weeks of age using growth, hematology, clinical chemistry, plasma immunoglobulins, and corticosterone as variables. The second and third studies were done to compare the effects of LPS on control and HESM fed birds at 5 weeks of age following at 4 and 24 h of treatment where the HESM was also sterilized with ethanol to deplete bacterial factors. HESM supplement caused weight gain in 2 experiments and decreased blood corticosterone concentrations. While LPS caused a significant loss in body weight at 24 h following its administration, the HESM supplemented birds showed significantly less body weight loss compared with the control fed birds. The WBC, heterophil/lymphocyte ratio, and the levels of IgG were low in chickens fed diets with HESM supplement compared with control diet group. LPS challenge increased the expression of pro-inflammatory cytokine gene IL-6 but the HESM fed birds showed its effect curtailed, also, which also, favored the up-regulation of anti-inflammatory genes compared with control diet fed chickens. Post hatch supplementation of HESM appears to improve performance, modulate immunity, and increase resistance of chickens to endotoxin. C1 [Makkar, S. K.; Rath, N. C.; Huff, G. R.; Donoghue, A. M.] ARS, Poultry Prod & Prod Safety Res Unit, USDA, Fayetteville, AR 72701 USA. [Makkar, S. K.; Packialakshmi, B.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Zhou, Z. Y.] Southwest Univ, Dept Vet Med, Rongchang Campus, Chongqing, Rongchang Count, Peoples R China. RP Rath, NC (reprint author), ARS, Poultry Prod & Prod Safety Res Unit, USDA, Fayetteville, AR 72701 USA. EM narayan.rath@ars.usda.gov NR 58 TC 1 Z9 1 U1 4 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 27 PY 2016 VL 11 IS 7 AR e0159433 DI 10.1371/journal.pone.0159433 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IJ UT WOS:000381515900038 PM 27463239 ER PT J AU Xin, XX Chen, Y Chen, D Xiao, F Parnell, LD Zhao, J Liu, L Ordovas, JM Lai, CQ Shen, LR AF Xin, Xiao-xuan Chen, Yong Chen, Di Xiao, Fa Parnell, Laurence D. Zhao, Jing Liu, Liang Ordovas, Jose M. Lai, Chao-Qiang Shen, Li-rong TI Supplementation with Major Royal-Jelly Proteins Increases Lifespan, Feeding, and Fecundity in Drosophila SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Drosophila; major royal jelly proteins; lifespan; fecundity and feeding; antioxidation activity; Egfr-mediated signaling pathway ID APIS-MELLIFERA-L; HONEYBEE; GROWTH; BEE; DIFFERENTIATION; MELANOGASTER; RESTRICTION; ANTIOXIDANT; PERFORMANCE; MECHANISMS AB The major royal-jelly proteins (MRJPs) are the main constituents responsible for the specific physiological role of royal jelly (RJ) in honeybees. Male and female Drosophila flies were fed diets containing either no MRJPs (A) or casein (B) at 1.25% (w/w) of diet or MRJPs at 1.25% (C), 2.50% (D), or 5.00% (E). Diets B, C, D, and E increased mean lifespan by 4.3%, 9.0%, 12.4%, and 13.9% in males and by 5.8%, 9.7%, 20.0%, and 11.8% in females in comparison to results from diet A, respectively. The diet supplemented with 2.50% MRJPs seems to have the optimal dose to improve both physiological and biochemical measures related to aging in both sexes. Interestingly, lifespan extension by MRJPs in Drosophila was positively associated with feeding and fecundity and up-regulation of copper and zinc superoxide dismutase (CuZn SOD) and the Egfr-mediated signaling pathway. This study provides strong evidence that MRJPs are important components of RJ for prolonging lifespan in Drosophila. C1 [Xin, Xiao-xuan; Chen, Yong; Chen, Di; Xiao, Fa; Shen, Li-rong] Zhejiang Univ, Fuli Inst Food Sci, Dept Food Sci & Nutr, Zhejiang Key Lab Agrofood Proc, Hangzhou 310058, Zhejiang, Peoples R China. [Parnell, Laurence D.; Ordovas, Jose M.; Lai, Chao-Qiang] Tufts Univ, USDA ARS, JM USDA Human Nutr Res Ctr Aging, Nutr Genom Lab, Boston, MA 02111 USA. [Zhao, Jing; Liu, Liang] Univ Georgia, Dept Stat, Athens, GA 30602 USA. RP Shen, LR (reprint author), Zhejiang Univ, Fuli Inst Food Sci, Dept Food Sci & Nutr, Zhejiang Key Lab Agrofood Proc, Hangzhou 310058, Zhejiang, Peoples R China.; Lai, CQ (reprint author), Tufts Univ, USDA ARS, JM USDA Human Nutr Res Ctr Aging, Nutr Genom Lab, Boston, MA 02111 USA. EM chaoqiang.lai@ars.usda.gov; shenlirong@zju.edu.cn OI Chen, Di/0000-0002-8060-4706 FU Important Scientific and Technical Innovation Project of Hangzhou [20131812A25]; Foundation of Fuli Institute of Food Science, Zhejiang University [KY201404]; National Natural Science Foundation of China [31271848]; U.S. Department of Agriculture, Agriculture Research Service [58-1950-9-001] FX This work was supported by the Important Scientific and Technical Innovation Project of Hangzhou (no. 20131812A25); the Foundation of Fuli Institute of Food Science, Zhejiang University (no. KY201404); the National Natural Science Foundation of China (no. 31271848); and the U.S. Department of Agriculture, Agriculture Research Service (58-1950-9-001). NR 52 TC 0 Z9 0 U1 14 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUL 27 PY 2016 VL 64 IS 29 BP 5803 EP 5812 DI 10.1021/acs.jafc.6b00514 PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA DS4EW UT WOS:000380735200004 PM 27388939 ER PT J AU Martinelli, F Dolan, D Fileccia, V Reagan, RL Phu, M Spann, TM McCollum, TG Dandekar, AM AF Martinelli, Federico Dolan, David Fileccia, Veronica Reagan, Russell L. Phu, My Spann, Timothy M. McCollum, Thomas G. Dandekar, Abhaya M. TI Molecular Responses to Small Regulating Molecules against Huanglongbing Disease SO PLOS ONE LA English DT Article ID CANDIDATUS-LIBERIBACTER-ASIATICUS; ARABIDOPSIS-THALIANA; NONHOST RESISTANCE; DEFENSE RESPONSES; CITRUS-SINENSIS; GENE-EXPRESSION; INFECTION; PLANTS; IDENTIFICATION; TRANSCRIPTOME AB Huanglongbing (HLB; citrus greening) is the most devastating disease of citrus worldwide. No cure is yet available for this disease and infected trees generally decline after several months. Disease management depends on early detection of symptoms and chemical control of insect vectors. In this work, different combinations of organic compounds were tested for the ability to modulate citrus molecular responses to HLB disease beneficially. Three small-molecule regulating compounds were tested: 1) L-arginine, 2) 6-benzyl-adenine combined with gibberellins, and 3) sucrose combined with atrazine. Each treatment contained K-phite mineral solution and was tested at two different concentrations. Two trials were conducted: one in the greenhouse and the other in the orchard. In the greenhouse study, responses of 42 key genes involved in sugar and starch metabolism, hormone-related pathways, biotic stress responses, and secondary metabolism in treated and untreated mature leaves were analyzed. TGA5 was significantly induced by arginine. Benzyladenine and gibberellins enhanced two important genes involved in biotic stress responses: WRKY54 and WRKY59. Sucrose combined with atrazine mainly upregulated key genes involved in carbohydrate metabolism such as sucrose-phosphate synthase, sucrose synthase, starch synthase, and a-amylase. Atrazine also affected expression of some key genes involved in systemic acquired resistance such as EDS1, TGA6, WRKY33, and MYC2. Several treatments upregulated HSP82, which might help protect protein folding and integrity. A subset of key genes was chosen as biomarkers for molecular responses to treatments under field conditions. GPT2 was downregulated by all small-molecule treatments. Arginine-induced genes involved in systemic acquired resistance included PR1, WRKY70, and EDS1. These molecular data encourage long-term application of treatments that combine these regulating molecules in field trials. C1 [Martinelli, Federico; Fileccia, Veronica] Dipartimento Sci Agr & Forestali, Palermo, Italy. [Martinelli, Federico; Fileccia, Veronica] Euromediterranean Inst Sci & Technol IEMEST, Palermo, Italy. [Dolan, David; Reagan, Russell L.; Phu, My; Dandekar, Abhaya M.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Spann, Timothy M.] Calif Avocado Commiss, Irvine, CA USA. [McCollum, Thomas G.] USDA ARS, US Hort Res Lab, Ft Pierce, FL USA. RP Dandekar, AM (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM amdandekar@ucdavis.edu RI Martinelli, Federico/F-7697-2013 FU Citrus Research and Development Foundation [CATAP09-305]; University of Palermo FX The research reported in this publication was supported by grant# CATAP09-305 received from the Citrus Research and Development Foundation and supported the work conducted by all of the authors except Veronica Fileccia who was supported by the University of Palermo. It should be noted that in either case the funders had no role in study design, data collection and analysis, decision to publish, or preparation of this manuscript. NR 47 TC 0 Z9 0 U1 11 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 26 PY 2016 VL 11 IS 7 AR e0159610 DI 10.1371/journal.pone.0159610 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IH UT WOS:000381515600036 PM 27459099 ER PT J AU Olson, ME Sankaran, RP Fahey, JW Grusak, MA Odee, D Nouman, W AF Olson, Mark E. Sankaran, Renuka P. Fahey, Jed W. Grusak, Michael A. Odee, David Nouman, Wasif TI Leaf Protein and Mineral Concentrations across the "Miracle Tree" Genus Moringa SO PLOS ONE LA English DT Article ID CHILD UNDERNUTRITION; ANTIOXIDANT ACTIVITY; SOLUBLE-PROTEIN; OLEIFERA LEAVES; SEED OIL; GROWTH; PARTS; WATER; BRASSICALES; STRATEGIES AB The moringa tree Moringa oleifera is a fast-growing, drought-resistant tree cultivated across the lowland dry tropics worldwide for its nutritious leaves. Despite its nutritious reputation, there has been no systematic survey of the variation in leaf nutritional quality across M. oleifera grown worldwide, or of the other species of the genus. To guide informed use of moringa, we surveyed protein, macro-, and micro-nutrients across 67 common garden samples of 12 Moringa taxa, including 23 samples of M. oleifera. Moringa oleifera, M. concanensis, M. stenopetala, an M. concanensis X oleifera hybrid, and M. longituba were highest in protein, with M. ruspoliana having the highest calcium levels. A protein-dry leaf mass tradeoff may preclude certain breeding possibilities, e.g. maximally high protein with large leaflets. These findings identify clear priorities and limitations for improved moringa varieties with traits such as high protein, calcium, or ease of preparation. C1 [Olson, Mark E.] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City, DF, Mexico. [Sankaran, Renuka P.] CUNY, Dept Biol Sci, Lehman Coll, Bronx, NY 10453 USA. [Sankaran, Renuka P.] CUNY, Grad Sch, New York, NY 10017 USA. [Sankaran, Renuka P.] CUNY, Univ Ctr, New York, NY 10017 USA. [Fahey, Jed W.] Johns Hopkins Univ, Sch Med, Dept Med, Cullman Chemoprotect Ctr, Baltimore, MD 21205 USA. [Grusak, Michael A.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA,ARS, Houston, TX 77030 USA. [Odee, David] Kenya Forestry Res Inst, Biotechnol Lab, Nairobi, Kenya. [Nouman, Wasif] Bahauddin Zakariya Univ, Dept Forestry Range & Wildlife Management, Multan, Pakistan. RP Sankaran, RP (reprint author), CUNY, Dept Biol Sci, Lehman Coll, Bronx, NY 10453 USA.; Sankaran, RP (reprint author), CUNY, Grad Sch, New York, NY 10017 USA.; Sankaran, RP (reprint author), CUNY, Univ Ctr, New York, NY 10017 USA. EM renuka.sankaran@lehman.cuny.edu FU Trees for Life; Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica of the Universidad Nacional Autonoma de Mexico [IT200515]; Lewis and Dorothy Cullman Foundation; PSC at City University of New York; Agricultural Research Service [58-6250-0-008] FX Trees for Life (www.treesforlife.org) provided funding to MO to bring the plants studied to Mexico, to prepare the shadehouse, prepare the land, plant, and maintain the common garden. Trees for Life is a humanitarian NGO that uses moringa as a crucial low cost nutritional resource in poor dry tropical communities worldwide. They provided the authors with the mandate to learn about moringa protein to guide selection of the optimal variants for addressing protein malnutrition. The Programa de Apoyo a Proyectos de Investigacion e Innovacion Tecnologica (http://dgapa.unam.mx/html/papiit/papit.html) of the Universidad Nacional Autonoma de Mexico grant IT200515 has provided funds to MO for fieldwork and collection maintenance. The Lewis and Dorothy Cullman Foundation (http://www.lewiscullman.com/) have provided funding to JWF for his research on glucosinolates and other compounds across the mustard oil plants. These funds helped make BCA measurements of protein possible. PSC at City University of New York (http://www.psc-cuny.org/) provided some funds for reagents to RPS. Funding was also provided by the Agricultural Research Service (58-6250-0-008). Except for that mentioned for Trees for Life, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 0 Z9 0 U1 11 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 26 PY 2016 VL 11 IS 7 AR e0159782 DI 10.1371/journal.pone.0159782 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IH UT WOS:000381515600047 PM 27459315 ER PT J AU Rassati, D Faccoli, M Haack, RA Rabaglia, RJ Toffolo, EP Battisti, A Marini, L AF Rassati, Davide Faccoli, Massimo Haack, Robert A. Rabaglia, Robert J. Toffolo, Edoardo Petrucco Battisti, Andrea Marini, Lorenzo TI Bark and Ambrosia Beetles Show Different Invasion Patterns in the USA SO PLOS ONE LA English DT Article ID EXPLAINING BETA DIVERSITY; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; WOOD-BORING BEETLES; BIOLOGICAL INVASIONS; UNITED-STATES; EXOTIC BARK; PINE-BEETLE; NORTH-AMERICA; FOREST; ECOLOGY AB Non-native bark and ambrosia beetles represent a threat to forests worldwide. Their invasion patterns are, however, still unclear. Here we investigated first, if the spread of non-native bark and ambrosia beetles is a gradual or a discontinuous process; second, which are the main correlates of their community structure; third, whether those correlates correspond to those of native species. We used data on species distribution of non-native and native scolytines in the continental 48 USA states. These data were analyzed through a beta-diversity index, partitioned into species richness differences and species replacement, using Mantel correlograms and non-metric multidimensional scaling (NMDS) ordination for identifying spatial patterns, and regression on distance matrices to test the association of climate (temperature, rainfall), forest (cover area, composition), geographical (distance), and human-related (import) variables with beta-diversity components. For both non-native bark and ambrosia beetles, beta-diversity was mainly composed of species richness difference than species replacement. For non-native bark beetles, a discontinuous invasion process composed of long distance jumps or multiple introduction events was apparent. Species richness differences were primarily correlated with differences in import values while temperature was the main correlate of species replacement. For non-native ambrosia beetles, a more continuous invasion process was apparent, with the pool of non-native species arriving in the coastal areas that tended to be filtered as they spread to interior portions of the continental USA. Species richness differences were mainly correlated with differences in rainfall among states, while rainfall and temperature were the main correlates of species replacement. Our study suggests that the different ecology of bark and ambrosia beetles influences their invasion process in new environments. The lower dependency that bark beetles have on climate allowed them to potentially colonize more areas within the USA, while non-native ambrosia beetles, being dependent on rainfall, are typically filtered by the environment. C1 [Rassati, Davide; Faccoli, Massimo; Toffolo, Edoardo Petrucco; Battisti, Andrea; Marini, Lorenzo] Univ Padua, Dept Agron Food Nat Resources Anim & Environm DAF, Legnaro, PD, Italy. [Haack, Robert A.] US Forest Serv, USDA, Northern Res Stn, Lansing, MI USA. [Rabaglia, Robert J.] US Forest Serv, USDA, Forest Hlth Protect, Washington, DC USA. RP Rassati, D (reprint author), Univ Padua, Dept Agron Food Nat Resources Anim & Environm DAF, Legnaro, PD, Italy. EM davide.rassati@unipd.it RI Marini, Lorenzo/B-1119-2009 OI Marini, Lorenzo/0000-0001-7429-7685 FU University of Padova FX The authors received no specific funding for this work. University of Padova provided support in the form of salaries for authors DR, MF, EPT, AB, LM, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 81 TC 0 Z9 0 U1 21 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 26 PY 2016 VL 11 IS 7 AR e0158519 DI 10.1371/journal.pone.0158519 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5IH UT WOS:000381515600007 PM 27459191 ER PT J AU Khazaei, H Caron, CT Fedoruk, M Diapari, M Vandenberg, A Coyne, CJ Mcgee, R Bett, KE AF Khazaei, Hamid Caron, Carolyn T. Fedoruk, Michael Diapari, Marwan Vandenberg, Albert Coyne, Clarice J. McGee, Rebecca Bett, Kirstin E. TI Genetic Diversity of Cultivated Lentil (Lens culinaris Medik.) and Its Relation to the World's Agro-ecological Zones SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE lentil; genetic diversity; population structure; germplasm; SNP markers; agro-ecological zones ID SNP MARKERS; LANDRACES; COLLECTION; AGRICULTURE; PHYLOGENY; SOFTWARE; ORIGINS; AFLP; SSR AB Assessment of genetic diversity and population structure of germplasm collections plays a critical role in supporting conservation and crop genetic enhancement strategies. We used a cultivated lentil (Lens culinaris Medik.) collection consisting of 352 accessions originating from 54 diverse countries to estimate genetic diversity and genetic structure using 1194 polymorphic single nucleotide polymorphism (SNP) markers which span the lentil genome. Using principal coordinate analysis, population structure analysis and UPGMA cluster analysis, the accessions were categorized into three major groups that prominently reflected geographical origin (world's agro-ecological zones). The three clusters complemented the origins, pedigrees, and breeding histories of the germplasm. The three groups were (a) South Asia (sub-tropical savannah), (b) Mediterranean, and (c) northern temperate. Based on the results from this study, it is also clear that breeding programs still have considerable genetic diversity to mine within the cultivated lentil, as surveyed South Asian and Canadian germplasm revealed narrow genetic diversity. C1 [Khazaei, Hamid; Caron, Carolyn T.; Fedoruk, Michael; Diapari, Marwan; Vandenberg, Albert; Bett, Kirstin E.] Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK, Canada. [Diapari, Marwan] Agr & Agri Food Canada, London Res & Dev Ctr, London, ON, Canada. [Coyne, Clarice J.; McGee, Rebecca] USDA ARS, Pullman, WA USA. RP Bett, KE (reprint author), Univ Saskatchewan, Dept Plant Sci, Saskatoon, SK, Canada. EM k.bett@usask.ca OI Khazaei, Hamid/0000-0002-5202-8764 FU Natural Sciences and Engineering Research Council of Canada (NSERC); Saskatchewan Pulse Growers FX This research was supported by Natural Sciences and Engineering Research Council of Canada (NSERC) and the Saskatchewan Pulse Growers. The authors would like to acknowledge Rob Stonehouse and Lacey-Anne Sanderson for their kind technical support during the experiments. NR 49 TC 0 Z9 0 U1 14 U2 19 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUL 26 PY 2016 VL 7 AR 1093 DI 10.3389/fpls.2016.01093 PG 7 WC Plant Sciences SC Plant Sciences GA DR9MT UT WOS:000380221500001 PM 27507980 ER PT J AU Kluever, BM Gese, EM Dempsey, SJ AF Kluever, Bryan M. Gese, Eric M. Dempsey, Steven J. TI The influence of wildlife water developments and vegetation on rodent abundance in the Great Basin Desert SO JOURNAL OF MAMMALOGY LA English DT Article DE arid; guzzlers; indirect effect; precipitation; small mammal; total abundance; vegetation cover ID SMALL MAMMAL COMMUNITY; INTRAGUILD PREDATION; SOUTHWESTERN ARIZONA; PRAIRIE RODENTS; LEVEL RESPONSES; POPULATIONS; COMPETITION; ECOSYSTEM; HABITATS; DYNAMICS AB Rodent communities have multiple functions including comprising a majority of the mammalian diversity within an ecosystem, providing a significant portion of the available biomass consumed by predators, and contributing to ecosystem services. Despite the importance of rodent communities, few investigations have explored the effects of increasing anthropogenic modifications to the landscape on rodents. Throughout the western United States, the construction of artificial water developments to benefit game species is commonplace. While benefits for certain species have been documented, several researchers recently hypothesized that these developments may cause unintentional negative effects to desert-adapted species and communities. To test this idea, we sampled rodents near to and distant from wildlife water developments over 4 consecutive summers. We employed an asymmetrical before-after-control-impact (BACI) design with sampling over 4 summers to determine if water developments influenced total rodent abundance. We performed an additional exploratory analysis to determine if factors other than free water influenced rodent abundance. We found no evidence that water developments impacted rodent abundance. Rodent abundance was primarily driven by vegetation type and year of sampling. Our findings suggested that water developments on our study area do not represent a significant disturbance to rodent abundance and that rodent abundance was influenced by the vegetative community and temporal factors linked to precipitation and primary plant production. Our findings represent one of the 1st efforts to determine the effects of an anthropogenic activity on the rodent community utilizing a manipulation design. C1 [Kluever, Bryan M.; Dempsey, Steven J.] Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. [Gese, Eric M.] Utah State Univ, USDA, Anim & Plant Hlth Inspect Serv,Dept Wildland Reso, Wildlife Serv,Natl Wildlife Res Ctr, Logan, UT 84322 USA. RP Kluever, BM (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. EM klueverb@gmail.com FU Department of Defense; U.S. Army Dugway Proving Ground, Environmental Programs, Dugway, Utah; U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center; Utah State University, Logan, Utah; Quinney College of Natural Resources, Utah State University, Logan, Utah; TE Inc.; American Society of Mammalogists; Utah Chapter of the Wildlife Society; Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources, Salt Lake City, Utah FX Funding and logistical support provided by the Department of Defense, U.S. Army Dugway Proving Ground, Environmental Programs, Dugway, Utah, and the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, and Utah State University, Logan, Utah. Additional funding provided by the Quinney College of Natural Resources, Utah State University, Logan, Utah, T&E Inc., American Society of Mammalogists, the Utah Chapter of the Wildlife Society, and the Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources, Salt Lake City, Utah. We thank R. Knight for providing continual support for the project and review of the manuscript. We also thank B. Smith, A. Hodge, C. Crawford, L. Card, M. Cent, A. Reyer, C. Hansen, J. Fasig, W. Knowlton, W. Wright, C. Perkins, S. McEachin, J. DeCotis, and M. Richmond for field assistance. L. Hall provided water development camera trapping summaries. The comments provided by 2 anonymous reviewers greatly improved the quality of the manuscript. NR 85 TC 0 Z9 0 U1 6 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2372 EI 1545-1542 J9 J MAMMAL JI J. Mammal. PD JUL 25 PY 2016 VL 97 IS 4 BP 1209 EP 1218 DI 10.1093/jmammal/gyw077 PG 10 WC Zoology SC Zoology GA DV9LN UT WOS:000383262100018 ER PT J AU Houtman, CJ Kitin, P Houtman, JCD Hammel, KE Hunt, CG AF Houtman, Carl J. Kitin, Peter Houtman, Jon C. D. Hammel, Kenneth E. Hunt, Christopher G. TI Acridine Orange Indicates Early Oxidation of Wood Cell Walls by Fungi SO PLOS ONE LA English DT Article ID WHITE-ROT BASIDIOMYCETE; CERIPORIOPSIS-SUBVERMISPORA; PULP FIBERS; LIGNIN; MECHANISM; AGGREGATION; MICROSCOPY; COMPLEXES; TITRATION; CELLULOSE AB Colonization of wood blocks by brown and white rot fungi rapidly resulted in detectable wood oxidation, as shown by a reduced phloroglucinol response, a loss of autofluorescence, and acridine orange (AO) staining. This last approach is shown to provide a novel method for identifying wood oxidation. When lignin was mildly oxidized, the association between AO and lignin was reduced such that stained wood sections emitted less green light during fluorescence microscopy. This change was detectable after less than a week, an interval that past work has shown to be too short for significant delignification of wood. Although fungal hyphae were observed in only a few wood lumina, oxidation was widespread, appearing relatively uniform over regions several hundred micrometers from the hyphae. This observation suggests that both classes of fungi release low molecular weight mild oxidants during the first few days of colonization. C1 [Houtman, Carl J.; Hammel, Kenneth E.; Hunt, Christopher G.] USDA Forest Serv, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Kitin, Peter; Hammel, Kenneth E.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Houtman, Jon C. D.] Univ Iowa, Dept Microbiol, Iowa City, IA 52242 USA. RP Houtman, CJ (reprint author), USDA Forest Serv, Forest Prod Lab, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM choutman@fs.fed.us RI Hammel, Kenneth/G-1890-2011; OI Hammel, Kenneth/0000-0002-2935-5847; Houtman, Jon/0000-0002-5307-9854 FU US Department of Energy [DE-AI02-07ER64491, DE-SC0006929] FX Funding was provided by US Department of Energy grants DE-AI02-07ER64491 (CGH and KEH) and DE-SC0006929 (KEH, CGH, and CJH). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 49 TC 0 Z9 0 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 25 PY 2016 VL 11 IS 7 AR e0159715 DI 10.1371/journal.pone.0159715 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5ID UT WOS:000381515200040 PM 27454126 ER PT J AU Velazquez-Salinas, L Zarate, S Eschbaumer, M Lobo, FP Gladue, DP Arzt, J Novella, IS Rodriguez, LL AF Velazquez-Salinas, Lauro Zarate, Selene Eschbaumer, Michael Lobo, Francisco Pereira Gladue, Douglas P. Arzt, Jonathan Novella, Isabel S. Rodriguez, Luis L. TI Selective Factors Associated with the Evolution of Codon Usage in Natural Populations of Arboviruses SO PLOS ONE LA English DT Article ID VESICULAR STOMATITIS-VIRUS; INSECT FLAVIVIRUS; RNA VIRUSES; BIAS; PATTERNS; ALTERNATION; INFECTION; MOSQUITOS; GENOMES; DIPTERA AB Arboviruses (arthropod borne viruses) have life cycles that include both vertebrate and invertebrate hosts with substantial differences in vector and host specificity between different viruses. Most arboviruses utilize RNA for their genetic material and are completely dependent on host tRNAs for their translation, suggesting that virus codon usage could be a target for selection. In the current study we analyzed the relative synonymous codon usage (RSCU) patterns of 26 arboviruses together with 25 vectors and hosts, including 8 vertebrates and 17 invertebrates. We used hierarchical cluster analysis (HCA) and principal component analysis (PCA) to identify trends in codon usage. HCA demonstrated that the RSCU of arboviruses reflects that of their natural hosts, but not that of dead-end hosts. Of the two major components identified by PCA, the first accounted for 62.1% of the total variance, and among the 59 codons analyzed in this study, the leucine codon CTG had the highest correlation with the first principal component, however isoleucine had the highest correlation during amino acid analysis. Nucleotide and dinucleotide composition were the variables that explained most of the total codon usage variance. The results suggest that the main factors driving the evolution of codon usage in arboviruses is based on the nucleotide and dinucleotide composition present in the host. Comparing codon usage of arboviruses and potential vector hosts can help identifying potential vectors for emerging arboviruses. C1 [Velazquez-Salinas, Lauro; Eschbaumer, Michael; Gladue, Douglas P.; Arzt, Jonathan; Rodriguez, Luis L.] ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Orient Point, NY 11957 USA. [Velazquez-Salinas, Lauro; Eschbaumer, Michael] ORISE, Oak Ridge, TN 37830 USA. [Zarate, Selene] Autonomous Univ Mexico City, Genom Sci Program, Mexico City, DF, Mexico. [Lobo, Francisco Pereira] Empresa Brasileira Pesquisa Agr Embrapa, Lab Multiusuario Bioinformat, Embrapa Informat Agr, Campinas, SP, Brazil. [Novella, Isabel S.] Univ Toledo, Dept Med Microbiol & Immunol, Coll Med & Life Sci, 2801 W Bancroft St, Toledo, OH 43606 USA. RP Velazquez-Salinas, L (reprint author), ARS, Foreign Anim Dis Res Unit, USDA, Plum Isl Anim Dis Ctr, Orient Point, NY 11957 USA.; Velazquez-Salinas, L (reprint author), ORISE, Oak Ridge, TN 37830 USA. EM lauro.velazquez@ars.usda.gov OI Arzt, Jonathan/0000-0002-7517-7893 NR 51 TC 0 Z9 0 U1 1 U2 1 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 25 PY 2016 VL 11 IS 7 AR e0159943 DI 10.1371/journal.pone.0159943 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DT5ID UT WOS:000381515200060 PM 27455096 ER PT J AU Wilhelm, C Harrison, OJ Schmitt, V Pelletier, M Spencer, SP Urban, JF Ploch, M Ramalingam, TR Siegel, RM Belkaid, Y AF Wilhelm, Christoph Harrison, Oliver J. Schmitt, Vanessa Pelletier, Martin Spencer, Sean P. Urban, Joseph F., Jr. Ploch, Michelle Ramalingam, Thirumalai R. Siegel, Richard M. Belkaid, Yasmine TI Critical role of fatty acid metabolism in ILC2-mediated barrier protection during malnutrition and helminth infection SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article ID INNATE LYMPHOID-CELLS; RETINOIC ACID; T-CELLS; IMMUNITY; ALPHA; INFLAMMATION; ADAPTATION; ACTIVATION; LIPOLYSIS; TISSUE AB Innate lymphoid cells (ILC) play an important role in many immune processes, including control of infections, inflammation, and tissue repair. To date, little is known about the metabolism of ILC and whether these cells can metabolically adapt in response to environmental signals. Here we show that type 2 innate lymphoid cells (ILC2), important mediators of barrier immunity, predominantly depend on fatty acid (FA) metabolism during helminth infection. Further, in situations where an essential nutrient, such as vitamin A, is limited, ILC2 sustain their function and selectively maintain interleukin 13 (IL-13) production via increased acquisition and utilization of FA. Together, these results reveal that ILC2 preferentially use FAs to maintain their function in the context of helminth infection or malnutrition and propose that enhanced FA usage and FA-dependent IL-13 production by ILC2 could represent a host adaptation to maintain barrier immunity under dietary restriction. C1 [Wilhelm, Christoph; Harrison, Oliver J.; Spencer, Sean P.; Belkaid, Yasmine] NIAID, Mucosal Immunol Sect, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. [Ramalingam, Thirumalai R.] NIAID, Immunopathogenesis Sect, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA. [Pelletier, Martin; Ploch, Michelle; Siegel, Richard M.] NIAMSD, Immunoregulat Sect, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. [Wilhelm, Christoph; Schmitt, Vanessa] Univ Bonn, Univ Hosp Bonn, Inst Clin Chem & Clin Pharmacol, D-53127 Bonn, Germany. [Spencer, Sean P.] Univ Penn, Perelman Sch Med, Dept Pathol & Lab Med, Inst Immunol, Philadelphia, PA 19104 USA. [Urban, Joseph F., Jr.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Pelletier, Martin] Univ Laval, CHU Quebec, Ctr Rech, Dept Microbiol & Immunol, Quebec City, PQ G1V 4G2, Canada. [Spencer, Sean P.] Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA. RP Wilhelm, C; Belkaid, Y (reprint author), NIAID, Mucosal Immunol Sect, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.; Wilhelm, C (reprint author), Univ Bonn, Univ Hosp Bonn, Inst Clin Chem & Clin Pharmacol, D-53127 Bonn, Germany. EM christoph.wilhelm@uni-bonn.de; ybelkaid@niaid.nih.gov OI Spencer, Sean/0000-0002-7328-5399; Wilhelm, Christoph/0000-0002-9839-0291; Pelletier, Martin/0000-0001-5033-4947; Ploch, Michelle/0000-0003-2565-1750 FU Division of Intramural Research of the NIAID; Ministry for Science and Education of North-Rhine-Westfalia; Deutsche Forschungsgemeinschaft Excellence Cluster ImmunoSensation; Human Frontier Science Program; U.S. Department of Agriculture/Agricultural Research Service Project Plan [8040-51000-058-00D] FX This work was supported by the Division of Intramural Research of the NIAID, the Ministry for Science and Education of North-Rhine-Westfalia (C. Wilhelm), the Deutsche Forschungsgemeinschaft Excellence Cluster ImmunoSensation (C. Wilhelm), and the Human Frontier Science Program and by funds from the U.S. Department of Agriculture/Agricultural Research Service Project Plan #8040-51000-058-00D (J.F. Urban Jr.). The NIAID Division of Intramural Research Animal Care and Use Program, as part of the National Institutes of Health Intramural Research Program, approved all of the experimental procedures (protocol LP3D-7E). NR 32 TC 1 Z9 1 U1 3 U2 3 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 950 THIRD AVE, 2ND FLR, NEW YORK, NY 10022 USA SN 0022-1007 EI 1540-9538 J9 J EXP MED JI J. Exp. Med. PD JUL 25 PY 2016 VL 213 IS 8 BP 1409 EP 1418 DI 10.1084/jem.20151448 PG 10 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DS5VN UT WOS:000380851200004 PM 27432938 ER PT J AU Ricker, N Shen, SY Goordial, J Jin, S Fulthorpe, RR AF Ricker, N. Shen, S. Y. Goordial, J. Jin, S. Fulthorpe, R. R. TI PacBio SMRT assembly of a complex multi-replicon genome reveals chlorocatechol degradative operon in a region of genome plasticity SO GENE LA English DT Article DE Genome assembly; Burkholderia; Plasmid; Mobile elements; Chlorocatechol degradation genes; Next generation sequencing ID SP STRAIN B13; 2,4-DICHLOROPHENOXYACETIC ACID; ALCALIGENES-EUTROPHUS; BURKHOLDERIA; GENES; SEQUENCE; BACTERIA; EVOLUTION; ALIGNMENT; PLASMIDS AB We have sequenced a Burkholderia genome that contains multiple replicons and large repetitive elements that would make it inherently difficult to assemble by short read sequencing technologies. We illustrate how the integrated long read correction algorithms implemented through the PacBio Single Molecule Real-Time (SMRT) sequencing technology successfully provided a de novo assembly that is a reasonable estimate of both the gene content and genome organization without making any further modifications. This assembly is comparable to related organisms assembled by more labour intensive methods. Our assembled genome revealed regions of genome plasticity for further investigation, one of which harbours a chlorocatechol degradative operon highly homologous to those previously identified on globally ubiquitous plasmids. In an ideal world, this assembly would still require experimental validation to confirm gene order and copy number of repeated elements. However, we submit that particularly in instances where a polished genome is not the primary goal of the sequencing project, PacBio SMRT sequencing provides a financially viable option for generating a biologically relevant genome estimate that can be utilized by other researchers for comparative studies. (C) 2016 Elsevier B.V. All rights reserved. C1 [Ricker, N.; Shen, S. Y.; Jin, S.; Fulthorpe, R. R.] Univ Toronto Scarborough, Dept Phys & Environm Sci, 1095 Mil Trail, Scarborough, ON M1C 1A4, Canada. [Goordial, J.] McGill Univ, Dept Nat Resource Sci, Macdonald Campus,21111 Lakeshore Rd, Ste Anne De Bellevue, PQ H9X 3V9, Canada. [Ricker, N.] ARS, USDA, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. RP Fulthorpe, RR (reprint author), Univ Toronto Scarborough, Dept Phys & Environm Sci, 1095 Mil Trail, Scarborough, ON M1C 1A4, Canada. EM fulthorpe@utsc.utoronto.ca FU NSERC [RGPIN-2015-04344]; NSERC CGS-D Scholarship; Michael Smith Foreign Study Supplement FX The authors gratefully acknowledge Eric Collins at the University of Alaska Fairbanks and Tony (Heng) Qian for assistance with genome assembly and annotation. NR is grateful to Ann Provoost and Kristel Mijnendonckx at the Belgian Nuclear Research Centre (SCK.CEN) for providing C. metallidurans CH34 DNA and for assistance and guidance for the large plasmid extractions. B. phytofirmans PsJN was kindly provided by Angela Sessitsch of the Austrian Institute of Technology. Funding in the form of a NSERC (RGPIN-2015-04344) Discovery Grant to RF and a NSERC CGS-D Scholarship and Michael Smith Foreign Study Supplement to NR are gratefully acknowledged. The funding agency had no role in this study. NR 61 TC 1 Z9 1 U1 3 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD JUL 25 PY 2016 VL 586 IS 2 BP 239 EP 247 DI 10.1016/j.gene.2016.04.018 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA DM9OR UT WOS:000376696100007 PM 27063562 ER PT J AU Warren, SD Aguilera, LE Baggett, LS AF Warren, Steven D. Aguilera, Lorgio E. Baggett, L. Scott TI Directional orientation of reproductive tissue of Eulychnia breviflora (Cactaceae) in the hyperarid Atacama Desert SO REVISTA CHILENA DE HISTORIA NATURAL LA English DT Article DE Eulychnia breviflora; Energetic efficiency; Equatorial orientation; Flowers; Photosynthetically active radiation (PAR) ID CEPHALOCEREUS-COLUMNA-TRAJANI; PSEUDOCEPHALIUM ORIENTATION; PACHYCEREUS-PRINGLEI; INSECT POLLINATORS; FLOWER ORIENTATION; OPUNTIA-MICRODASYS; CHIHUAHUAN-DESERT; PAR INTERCEPTION; FRUIT ABORTION; NORTHERN CHILE AB Background: Many barrel or columnar cacti, including some in the Atacama Desert, produce their reproductive tissue at or near the terminal apices of solitary or minimally branched stems that lean toward the equator, reportedly to maximize exposure to photosynthetically active radiation (PAR). Those with lateral reproductive tissue, often produce the tissue on the equatorial side of the stems. An examination of the multi-stemmed, arbuscular cactus, Eulychnia breviflora, was made to determine if it follows the same general strategy. Methods: Individuals of the species were evaluated along a 100 km transect in the Atacama Desert. The position of all floral buds and open flowers was documented relative to the center of the plants and relative to the center of the individual stems on which they were located. Results: A highly significant majority of the reproductive tissue was located on the equatorial (north) side of the plant and on the equatorial (north) side of the stems on which it was found. Conclusion: Our explanation of the phenomenon differs from other researchers. Inasmuch as reproductive tissue contains little or no chlorophyll, we suggest that the flowers emerge from areas of the stems that receive abundant PAR, not because the reproductive tissue itself requires exposure to PAR. Because the translocation of photosynthates in cacti is difficult and energetically expensive, positioning of reproductive tissue in zones of the stems with high photosynthetic capacity is more energetically efficient. In addition, the Atacama Desert is not particularly warm. Exposure of flowers to solar radiation may produce a thermal reward for pollinators, in addition to any nectar rewards received. C1 [Warren, Steven D.] US Forest Serv, Rocky Mt Res Stn, 735 North 500 East, Provo, UT 84606 USA. [Aguilera, Lorgio E.] Univ La Serena, Dept Biol, La Serena, Chile. [Baggett, L. Scott] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Warren, SD (reprint author), US Forest Serv, Rocky Mt Res Stn, 735 North 500 East, Provo, UT 84606 USA. EM swarren02@fs.fed.us FU U.S. Forest Service, Rocky Mountain Research Station; Universidad de La Serena, Chile FX Salary for SDW and LSB was paid by the U.S. Forest Service, Rocky Mountain Research Station. Salary for LEA was paid by the Universidad de La Serena, Chile. Travel and field subsistence for SDW and LEA was paid by the U.S. Forest Service, Rocky Mountain Research Station. NR 48 TC 1 Z9 1 U1 2 U2 8 PU SOC BIOLGIA CHILE PI SANTIAGO PA CASILLA 16164, SANTIAGO 9, CHILE SN 0716-078X EI 0717-6317 J9 REV CHIL HIST NAT JI Rev. Chil. Hist. Nat. PD JUL 22 PY 2016 VL 89 AR 10 DI 10.1186/s40693-016-0060-z PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA DR9GI UT WOS:000380204700001 ER PT J AU Hao, GX Stover, E Gupta, G AF Hao, Guixia Stover, Ed Gupta, Goutam TI Overexpression of a Modified Plant Thionin Enhances Disease Resistance to Citrus Canker and Huanglongbing (HLB) SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Xanthomonas citri; Candidatus Liberibacter asiaticus; modified plant thionin; gene cloning and expression; disease resistance; transgenic plant ID CANDIDATUS LIBERIBACTER ASIATICUS; AXONOPODIS PV. CITRI; MAXIMUM-LIKELIHOOD; XANTHOMONAS-CITRI; SUSCEPTIBILITY; TRANSMISSION; ARABIDOPSIS; PROTEINS; DEFENSE; BARLEY AB Huanglongbing (HLB or citrus greening disease) caused by Candidatus Liberibacter asiaticus (Las) is a great threat to the US citrus industry. There are no proven strategies to eliminate HLB disease and no cultivar has been identified with strong HLB resistance. Citrus canker is also an economically important disease associated with a bacterial pathogen (Xanthornonas citri). In this study, we characterized endogenous citrus thionins and investigated their expression in different citrus tissues. Since no HLB-resistant citrus cultivars have been identified, we attempted to develop citrus resistant to both HLB and citrus canker through overexpression of a modified plant thionin. To improve effectiveness for disease resistance, we modified and synthesized the sequence encoding a plant thionin and cloned into the binary vector pBinPlus/ARS. The construct was then introduced into Agrobacterium strain EHA105 for citrus transformation. Transgenic Carrizo plants expressing the modified plant thionin were generated by Agrobactenum-mediated transformation. Successful transformation and transgene gene expression was confirmed by molecular analysis. Transgenic Carrizo plants expressing the modified thionin gene were challenged with X citri 3213 at a range of concentrations, and a significant reduction in canker symptoms and a decrease in bacterial growth were demonstrated compared to nontransgenic plants. Furthermore, the transgenic citrus plants were challenged with HLB via graft inoculation. Our results showed significant Las titer reduction in roots of transgenic Carrizo compared with control plants and reduced scion Las titer 12 months after graft inoculation. These data provide promise for engineering citrus disease resistance against HLB and canker. C1 [Hao, Guixia; Stover, Ed] ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. [Gupta, Goutam] Los Alamos Natl Lab, Los Alamos, NM USA. RP Hao, GX; Stover, E (reprint author), ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. EM guixia.hao@ars.usda.gov; ed.stover@ars.usda.gov FU Citrus Research and Development Foundation FX We thank Ellen Cochrane, Yolanda Avila, Jennifer Wildonger, and Megan Geraghty for their excellent technical assistance. Funding was provided by the Citrus Research and Development Foundation. We also thank Dr. Godfrey Miles and Dr. Bill Belknap for their critical reviews. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 45 TC 1 Z9 1 U1 22 U2 36 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUL 22 PY 2016 VL 7 AR 1078 DI 10.3389/fpls.2016.01078 PG 11 WC Plant Sciences SC Plant Sciences GA DR6KM UT WOS:000380010800001 PM 27499757 ER PT J AU Ammar, ED Ramos, JE Hall, DG Dawson, WO Shatters, RG AF Ammar, El-Desouky Ramos, John E. Hall, David G. Dawson, William O. Shatters, Robert G., Jr. TI Acquisition, Replication and Inoculation of Candidatus Liberibacter asiaticus following Various Acquisition Periods on Huanglongbing-Infected Citrus by Nymphs and Adults of the Asian Citrus Psyllid SO PLOS ONE LA English DT Article ID COCKERELLI HEMIPTERA TRIOZIDAE; DIAPHORINA-CITRI; GREENING DISEASE; SALIVARY-GLANDS; VECTOR; TRANSMISSION; EFFICIENCY; PATHOGEN; TOMATO; TIME AB The Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), is the primary vector of Candidatus Liberibacter asiaticus (Las) implicated as causative agent of citrus huanglongbing (citrus greening), currently the most serious citrus disease worldwide. Las is transmitted by D. citri in a persistent-circulative manner, but the question of replication of this bacterium in its psyllid vector has not been resolved. Thus, we studied the effects of the acquisition access period (AAP) by nymphs and adults of D. citri on Las acquisition, multiplication and inoculation/ transmission. D. citri nymphs or adults (previously non-exposed to Las) were caged on Las-infected citrus plants for an AAP of 1, 7 or 14 days. These ` Las-exposed' psyllids were then transferred weekly to healthy citrus or orange jasmine plants, and sampled via quantitative polymerase chain reaction (qPCR) analysis 1-42 days post-first access to diseased plants (padp); all tested nymphs became adults 7-14 days padp. Our results indicate that following 1 or 7 day AAP as nymphs 49-59% of Las-exposed psyllids became Las-infected (qPCR-positive), whereas only 8-29% of the psyllids were infected following 1-14 day AAP as adults. Q-PCR analysis also indicated that Las titer in the Las-exposed psyllids (relative to that of the psyllid S20 ribosomal protein gene) was: 1) significantly higher, and increasing at a faster rate, following Las acquisition as nymphs compared to that following Las acquisition as adults; 2) higher as post-acquisition time of psyllids on healthy plants increased reaching a peak at 14-28 days padp for nymphs and 21-35 days padp for adults, with Las titer decreasing or fluctuating after that; 3) higher with longer AAP on infected plants, especially with acquisition as adults. Our results strongly suggest that Las multiplies in both nymphs and adults of D. citri but attains much higher levels in a shorter period of time post-acquisition when acquired by nymphs than when acquired by adults, and that adults may require longer access to infected plants compared to nymphs for Las to reach higher levels in the vector. However, under the conditions of our experiments, only D. citri that had access to infected plants as nymphs were able to inoculate Las into healthy citrus seedlings or excised leaves. The higher probability of Las inoculation into citrus by psyllids when they have acquired this bacterium from infected plants during the nymphal rather than the adult stage, as reported by us and others, has significant implications in the epidemiology and control of this economically important citrus disease. C1 [Ammar, El-Desouky; Ramos, John E.; Hall, David G.; Shatters, Robert G., Jr.] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. [Ammar, El-Desouky; Dawson, William O.] Univ Florida, IFAS CREC, Lake Alfred, FL 33850 USA. RP Ammar, ED (reprint author), USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA.; Ammar, ED (reprint author), Univ Florida, IFAS CREC, Lake Alfred, FL 33850 USA. EM desoukyammar@gmail.com OI Dawson, Warrick/0000-0003-0050-6717 FU Florida Citrus Research and Development Foundation [Hall-93]; United States Department of Agriculture-Agricultural Research Service, Horticultural Research Laboratory, Fort Pierce, Florida, USA FX This work was supported by Florida Citrus Research and Development Foundation (Grant no: Hall-93 to DG Hall); United States Department of Agriculture-Agricultural Research Service, Horticultural Research Laboratory, Fort Pierce, Florida, USA. NR 44 TC 1 Z9 1 U1 9 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 21 PY 2016 VL 11 IS 7 AR e0159594 DI 10.1371/journal.pone.0159594 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS5CG UT WOS:000380797500101 PM 27441694 ER PT J AU Lim, CK Penesyan, A Hassan, KA Loper, JE Paulsen, IT AF Lim, Chee Kent Penesyan, Anahit Hassan, Karl A. Loper, Joyce E. Paulsen, Ian T. TI Disruption of Transporters Affiliated with Enantio-Pyochelin Biosynthesis Gene Cluster of Pseudomonas protegens Pf-5 Has Pleiotropic Effects SO PLOS ONE LA English DT Article ID MEDIATED IRON UPTAKE; SALICYLIC-ACID; SIDEROPHORE PYOCHELIN; MEMBRANE TRANSPORTERS; SYSTEMIC RESISTANCE; BACILLUS-SUBTILIS; FLUORESCENS PF-5; BOTRYTIS-CINEREA; AERUGINOSA; INDUCTION AB Pseudomonas protegens Pf-5 (formerly Pseudomonas fluorescens) is a biocontrol bacterium that produces the siderophore enantio-pyochelin under conditions of iron starvation in a process that is often accompanied by the secretion of its biosynthesis intermediates, salicylic acid and dihydroaeruginoic acid. In this study, we investigated whether several transporters that are encoded by genes within or adjacent to the enantio-pyochelin biosynthetic cluster, serve as efflux systems for enantio-pyochelin and/or its intermediates. In addition, we determined whether these transporters have broad substrates range specificity using a Phenotype Microarray system. Intriguingly, knockouts of the pchH and fetF transporter genes resulted in mutant strains that secrete higher levels of enantio-pyochelin as well as its intermediates salicylic acid and dihydroaeruginoic acid. Analyses of these mutants did not indicate significant change in transcription of biosynthetic genes involved in enantiopyochelin production. In contrast, the deletion mutant of PFL_3504 resulted in reduced transcription of the biosynthetic genes as well as decreased dihydroaeruginoic acid concentrations in the culture supernatant, which could either point to regulation of gene expression by the transporter or its role in dihydroaeruginoic acid transport. Disruption of each of the transporters resulted in altered stress and/or chemical resistance profile of Pf-5, which may reflect that these transporters could have specificity for rather a broad range of substrates. C1 [Lim, Chee Kent; Penesyan, Anahit; Hassan, Karl A.; Paulsen, Ian T.] Macquarie Univ, Dept Chem & Biomol Sci, Fac Sci & Engn, Sydney, NSW, Australia. [Loper, Joyce E.] Oregon State Univ, USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. [Loper, Joyce E.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Paulsen, IT (reprint author), Macquarie Univ, Dept Chem & Biomol Sci, Fac Sci & Engn, Sydney, NSW, Australia. EM ian.paulsen@mq.edu.au RI Penesyan, Anahit/A-9442-2012; Paulsen, Ian/K-3832-2012; OI Penesyan, Anahit/0000-0002-2502-0740; Paulsen, Ian/0000-0001-9015-9418; Hassan, Karl/0000-0003-2031-9679; Loper, Joyce/0000-0003-3501-5969 FU Australian Research Council [DP110102680, DP160103746]; National Institute of Food and Agriculture-Agriculture and Food Research Initiative Competitive Grants Program Grant [2011-67019-30192]; Macquarie University Research Excellence Scholarship FX The project was funded by the Australian Research Council-Discovery Grants DP110102680 (awarded to KAH and ITP), DP160103746 (awarded to KAH and ITP), and the National Institute of Food and Agriculture-Agriculture and Food Research Initiative Competitive Grants Program Grant no. 2011-67019-30192 (awarded to JEL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank Rachel Codd and Najwa Braich of University of Sydney for useful advice and experimental feasibility evaluation. We would like to convey our gratitude to Teresa A. Kidarsa and Jennifer Clifford (USDA) for giving technical advice on the construction of the deletion mutants and reading the manuscript respectively. We also thank Joanne Jamie, Meza Meyanungsang, Christine Turnbull, Adam Stow and Robert Willows from Macquarie University for providing access and assistance in the use of equipment. We acknowledge Ernest Lacey of Microbial Screening Technologies, Australia, for his generous gift of purified DHA. CKL was supported by the Macquarie University Research Excellence Scholarship. NR 61 TC 1 Z9 1 U1 2 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 21 PY 2016 VL 11 IS 7 AR e0159884 DI 10.1371/journal.pone.0159884 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DS5CG UT WOS:000380797500151 PM 27442435 ER PT J AU Torres-Rodriguez, L Garcia-Chavez, E Berhow, M de Mejia, EG AF Lucina Torres-Rodriguez, Maria Garcia-Chavez, Erika Berhow, Mark de Mejia, Elvira Gonzalez TI Anti-inflammatory and anti-oxidant effect of Calea urticifolia lyophilized aqueous extract on lipopolysaccharide-stimulated RAW 264.7 macrophages SO JOURNAL OF ETHNOPHARMACOLOGY LA English DT Article DE Calea urticifolia; Inflammation; Phenolic compounds; Nitric oxide; Reactive oxygen species; NF-kappa B ID NF-KAPPA-B; IN-VITRO; SESQUITERPENE LACTONE; INDUCED INFLAMMATION; INSULIN-RESISTANCE; ARTEMISIA-ANNUA; ADIPOSE-TISSUE; ACTIVATION; MECHANISMS; GERMACRANOLIDES AB Ethnopharmacological relevance: Calea urticifolia leaves are traditionally used as a remedy to treat gastric ulcers, diabetes, and inflammation by the Xi'uy ancient native community of San Luis Potosi, Mexico. Aim of the study: The aim was to assess the effects of the aqueous extract of the Mexican plant C urticifolia as anti-inflammatory and anti-oxidant using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and to provide evidence on the phenolic compounds. Materials and methods: RAW 264.7 macrophages were stimulated with 1 mu g/mL of LPS and treated with 10, 25 50, 75 y 100 mu g/mL of Calea urticifolia lyophilized aqueous extract (CuAqE). Nitric oxide (NO) release, tumor necrosis factor alpha, prostaglandin E-2 production, inducible nitric oxide synthase (iNOS), cyclooxygenase-2, nuclear factor-kappa B (NF-kappa B) p65, NF-kappa B p50 expression and reactive oxygen species (ROS) were measured; other pro-inflammatory proteins were measured with membrane antibody array. Phenolic compounds were analyzed by LC-ESI-MS. Results: Inflammation was inhibited by suppressing iNOS/NO pathway through inhibiting nucleus translocation of NF-kappa B p65 and p50 sub-units. ROS production was significantly (P < 0.05) inhibited in a dose-dependent manner in LPS-stimulated macrophages. Moreover, the expression of inflammatory markers was suppressed (34.5-88.3%) by CuAqE. A mix of caffeoylquinic acid derivatives and flavonoid-glycosides were found in CuAqE. Conclusion: Phenolic compounds in CuAgE such as caffeoylquinic acid derivatives and flavonoid glycosides could be responsible for inhibiting LPS-induced inflammation and oxidative stress by iNOS/NO pathway through suppressing NF-kappa B signaling pathway and by inhibition of ROS production in RAW 264.7 macrophages. Therefore, these results support the traditional knowledge of C urticifolia tea such as an anti-inflammatory and antioxidant agent. (C) 2016 Elsevier Ireland Ltd. All rights reserved. C1 [Lucina Torres-Rodriguez, Maria; Garcia-Chavez, Erika] Univ Autonoma San Luis Potosi, PMPCA, San Luis Potosi, SLP, Mexico. [Garcia-Chavez, Erika] Univ Autonoma San Luis Potosi, IIZD, San Luis Potosi, SLP, Mexico. [Berhow, Mark] USDA ARS, 1815 N Univ St, Peoria, IL 61604 USA. [Lucina Torres-Rodriguez, Maria; de Mejia, Elvira Gonzalez] Univ Illinois, Dept Food Sci & Human Nutr, Champaign, IL 61801 USA. RP de Mejia, EG (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, Champaign, IL 61801 USA. EM edemejia@illinois.edu FU Consejo Nacional de Ciencia y Tecnologia CONACyT Mexico [102165/165951]; University of Illinois at Urbana-Champaign (Hatch); PMPCA-IIZD at University of San Luis Potosi, Mexico [FAI-C15-FAI-04-28.82] FX Author Maria Lucina Torres acknowledges scholarship with register number 102165/165951 from Consejo Nacional de Ciencia y Tecnologia CONACyT Mexico. This work was supported by University of Illinois at Urbana-Champaign (Hatch) and PMPCA-IIZD (FAI-C15-FAI-04-28.82) at University of San Luis Potosi, Mexico. NR 37 TC 3 Z9 3 U1 11 U2 18 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0378-8741 J9 J ETHNOPHARMACOL JI J. Ethnopharmacol. PD JUL 21 PY 2016 VL 188 BP 266 EP 274 DI 10.1016/j.jep.2016.04.057 PG 9 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA DQ9WU UT WOS:000379560800026 ER PT J AU Ma, MF Wen, K Beier, RC Eremin, SA Li, CL Zhang, SX Shen, JZ Wang, ZH AF Ma, Mingfang Wen, Kai Beier, Ross C. Eremin, Sergei A. Li, Chenglong Zhang, Suxia Shen, Jianzhong Wang, Zhanhui TI Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE quantum dots; chemiluminescence resonance energy transfer; immunoassay; hapten functionalization; chemical contaminant ID LINKED-IMMUNOSORBENT-ASSAY; SULFONAMIDES; WATER; ANTIBODIES; PLATFORM; SAMPLES; MILK AB We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants. C1 [Ma, Mingfang; Li, Chenglong; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui] China Agr Univ, Coll Vet Med, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. [Wen, Kai; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui] Beijing Lab Food Qual & Safety, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. [Wen, Kai; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui] Beijing Key Lab Detect Technol Anim Derived Food, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. [Beier, Ross C.] USDA ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, 2881 F&B Rd, College Stn, TX 77845 USA. [Eremin, Sergei A.] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119992, Russia. RP Wang, ZH (reprint author), China Agr Univ, Coll Vet Med, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China.; Wang, ZH (reprint author), Beijing Lab Food Qual & Safety, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China.; Wang, ZH (reprint author), Beijing Key Lab Detect Technol Anim Derived Food, 2 Yuanmingyuan West Rd, Beijing 100193, Peoples R China. EM wangzhanhui@cau.edu.cn FU National Natural Science Foundation of China [31372475] FX The authors thank the National Natural Science Foundation of China (Grant 31372475) for financial support. NR 30 TC 0 Z9 0 U1 22 U2 22 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD JUL 20 PY 2016 VL 8 IS 28 BP 17745 EP 17750 DI 10.1021/acsami.6b04171 PG 6 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA DS0PE UT WOS:000380298400002 PM 27362827 ER PT J AU Wang, CC Schmid, CH Iversen, MD Harvey, WF Fielding, RA Driban, JB Price, LL Wong, JB Reid, KF Rones, R McAlindon, T AF Wang, Chenchen Schmid, Christopher H. Iversen, Maura D. Harvey, William F. Fielding, Roger A. Driban, Jeffrey B. Price, Lori Lyn Wong, John B. Reid, Kieran F. Rones, Ramel McAlindon, Timothy TI Comparative Effectiveness of Tai Chi Versus Physical Therapy for Knee Osteoarthritis SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID RANDOMIZED CONTROLLED-TRIAL; CLINICAL-TRIALS; FIBROMYALGIA; ARTHRITIS; OUTCOMES; PAIN; HIP; RELIABILITY; MANAGEMENT; STRENGTH AB Background: Few remedies effectively treat long-term pain and disability from knee osteoarthritis. Studies suggest that Tai Chi alleviates symptoms, but no trials have directly compared Tai Chi with standard therapies for osteoarthritis. Objective: To compare Tai Chi with standard physical therapy for patients with knee osteoarthritis. Design: Randomized, 52-week, single-blind comparative effectiveness trial. (ClinicalTrials.gov:NCT01258985) Setting: An urban tertiary care academic hospital. Patients: 204 participants with symptomatic knee osteoarthritis (mean age, 60 years; 70% women; 53% white). Intervention: Tai Chi (2 times per week for 12 weeks) or standard physical therapy (2 times per week for 6 weeks, followed by 6 weeks of monitored home exercise). Measurements: The primary outcome was Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score at 12 weeks. Secondary outcomes included physical function, depression, medication use, and quality of life. Results: At 12 weeks, the WOMAC score was substantially reduced in both groups (Tai Chi, 167 points [ 95% CI, 145 to 190 points]; physical therapy, 143 points [ CI, 119 to 167 points]). The between-group difference was not significant (24 points [ CI, - 10 to 58 points]). Both groups also showed similar clinically significant improvement in most secondary outcomes, and the benefits were maintained up to 52 weeks. Of note, the Tai Chi group had significantly greater improvements in depression and the physical component of quality of life. The benefit of Tai Chi was consistent across instructors. No serious adverse events occurred. Limitation: Patients were aware of their treatment group assignment, and the generalizability of the findings to other settings remains undetermined. Conclusion: Tai Chi produced beneficial effects similar to those of a standard course of physical therapy in the treatment of knee osteoarthritis. C1 [Wang, Chenchen] Tufts Med Ctr, 800 Washington St,Box 406, Boston, MA 02111 USA. [Wang, Chenchen] Tufts Univ, Sch Med, 800 Washington St,Box 406, Boston, MA 02111 USA. Tufts Univ, Boston, MA 02111 USA. Northeastern Univ, Boston, MA 02115 USA. Brigham & Womens Hosp, 75 Francis St, Boston, MA 02115 USA. Harvard Med Sch, Boston, MA USA. Ctr Mind Body Therapies, Boston, MA USA. Brown Univ, Sch Publ Hlth, Providence, RI 02912 USA. [Schmid, Christopher H.] Brown Univ, Sch Publ Hlth, Ctr Evidence Based Med, 121 South Main St,Box G-S121-8, Providence, RI 02912 USA. [Iversen, Maura D.] Northeastern Univ, Dept Phys Therapy Movement & Rehabil Sci, 360 Huntington Ave,Room 301c, Boston, MA 02115 USA. [Harvey, William F.; Driban, Jeffrey B.; McAlindon, Timothy] Tufts Univ, Sch Med, Tufts Med Ctr, Div Rheumatol, Box 406, Boston, MA 02111 USA. [Fielding, Roger A.; Reid, Kieran F.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. [Price, Lori Lyn] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Box 063, Boston, MA 02111 USA. [Wong, John B.] Tufts Med Ctr, Dept Med, Ctr Clin Decis Making, Box 302, Boston, MA 02111 USA. [Rones, Ramel] RR Prod, 17 Parker St, Lexington, MA 02421 USA. RP Wang, CC (reprint author), Tufts Med Ctr, 800 Washington St,Box 406, Boston, MA 02111 USA.; Wang, CC (reprint author), Tufts Univ, Sch Med, 800 Washington St,Box 406, Boston, MA 02111 USA. EM cwang2@tuftsmedicalcenter.org FU National Center for Complementary and Integrative Health of the National Institutes of Health FX National Center for Complementary and Integrative Health of the National Institutes of Health. NR 40 TC 7 Z9 7 U1 12 U2 17 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 USA SN 0003-4819 EI 1539-3704 J9 ANN INTERN MED JI Ann. Intern. Med. PD JUL 19 PY 2016 VL 165 IS 2 BP 77 EP + DI 10.7326/M15-2143 PG 15 WC Medicine, General & Internal SC General & Internal Medicine GA DS2GY UT WOS:000380583100014 PM 27183035 ER PT J AU Sandbulte, MR Gauger, PC Kitikoon, P Chen, HJ Perez, DR Roth, JA Vincent, AL AF Sandbulte, Matthew R. Gauger, Phillip C. Kitikoon, Pravina Chen, Hongjun Perez, Daniel R. Roth, James A. Vincent, Amy L. TI Neuraminidase inhibiting antibody responses in pigs differ between influenza A virus N2 lineages and by vaccine type SO VACCINE LA English DT Article DE Influenza A virus; Neuraminidase inhibiting antibodies; Live attenuated influenza virus; Whole inactivated virus; Mucosal immunity; Maternally derived antibodies ID ENHANCED RESPIRATORY-DISEASE; CROSS-PROTECTION; A(H3N2) VIRUS; LINKED LECTIN; SWINE; LIVE; EFFICACY; FERRETS; GENERATION; EVOLUTION AB The neuraminidase (NA) protein of influenza A viruses (IAV) has important functional roles in the viral replication cycle. Antibodies specific to NA can reduce viral replication and limit disease severity, but are not routinely measured. We analyzed NA inhibiting (NI) antibody titers in serum and respiratory specimens of pigs vaccinated with intramuscular whole-inactivated virus (WIV), intranasal live attenuated influenza virus (LAIV), and intranasal wild type (WT) IAV. NI titers were also analyzed in sera from an investigation of piglet vaccination in the presence of passive maternally-derived antibodies. Test antigens contained genetically divergent swine-lineage NA genes homologous or heterologous to the vaccines with mismatched hemagglutinin genes (HA). Naive piglets responded to WIV and LAIV vaccines and WT infection with strong homologous serum NI titers. Cross-reactivity to heterologous NAs depended on the degree of genetic divergence between the NA genes. Bronchoalveolar lavage specimens of LAIV and WT-immunized groups also had significant NI titers against the homologous antigen whereas the WIV group did not. Piglets of vaccinated sows received high levels of passive NI antibody, but their NI responses to homologous LAIV vaccination were impeded. These data demonstrate the utility of the enzyme-linked lectin assay for efficient NI antibody titration of serum as well as respiratory tract secretions. Swine IAV vaccines that induce robust NI responses are likely to provide broader protection against the diverse and rapidly evolving IAV strains that circulate in pig populations. Mucosal antibodies to NA may be one of the protective immune mechanisms induced by LAIV vaccines. Published by Elsevier Ltd. C1 [Sandbulte, Matthew R.; Roth, James A.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA USA. [Kitikoon, Pravina; Vincent, Amy L.] USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Gauger, Phillip C.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. [Chen, Hongjun; Perez, Daniel R.] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. [Sandbulte, Matthew R.; Kitikoon, Pravina] Merck Anim Hlth, Desoto, KS USA. [Chen, Hongjun] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China. [Perez, Daniel R.] Univ Georgia, Dept Populat Hlth, Athens, GA 30602 USA. RP Vincent, AL (reprint author), USDA ARS, Virus & Pr Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM Amy.Vincent@ars.usda.gov FU USDA-ARS [58-3625-0-612]; National Pork Board [11-061] FX This work was supported by funding from USDA-ARS cooperative agreement 58-3625-0-612 and National Pork Board grant 11-061. NR 35 TC 0 Z9 0 U1 4 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD JUL 19 PY 2016 VL 34 IS 33 BP 3773 EP 3779 DI 10.1016/j.vaccine.2016.06.001 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA DS1XN UT WOS:000380418600012 PM 27325350 ER PT J AU Davis, EW Weisberg, AJ Tabima, JF Grunwald, NJ Chang, JH AF Davis, Edward W., II Weisberg, Alexandra J. Tabima, Javier F. Grunwald, Niklaus J. Chang, Jeff H. TI Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria SO PEERJ LA English DT Article DE Taxonomy; Genomes; Molecular diagnostics; Multilocus sequence analysis; Average nucleotide identity; Agrobacterium; Rhodococcus ID MULTILOCUS SEQUENCE-ANALYSIS; HERBICOLA PV. GYPSOPHILAE; FORMING PANTOEA-AGGLOMERANS; PLANT-PATHOGENIC BACTERIA; SYRINGAE SUBSP-SAVASTANOI; ERWINIA-HERBICOLA; AGROBACTERIUM-TUMEFACIENS; RHODOCOCCUS-FASCIANS; PSEUDOMONAS-SYRINGAE; PHYLOGENETIC ANALYSIS AB Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of higi-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. C1 [Davis, Edward W., II; Weisberg, Alexandra J.; Tabima, Javier F.; Grunwald, Niklaus J.; Chang, Jeff H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Davis, Edward W., II; Grunwald, Niklaus J.; Chang, Jeff H.] Oregon State Univ, Mol & Cellular Biol Program, Corvallis, OR 97331 USA. [Grunwald, Niklaus J.; Chang, Jeff H.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Grunwald, Niklaus J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. RP Chang, JH (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA.; Chang, JH (reprint author), Oregon State Univ, Mol & Cellular Biol Program, Corvallis, OR 97331 USA.; Chang, JH (reprint author), Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. EM changj@science.oregonstate.edu OI Tabima, Javier/0000-0002-3603-2691; Grunwald, Niklaus/0000-0003-1656-7602 FU National Institute of Food and Agriculture; US Department of Agriculture award [2014-51181-22384]; USDA Agricultural Research Service Grant [5358-22000-039-00D]; USDA National Institute of Food and Agriculture Grant [2011-68004-30154]; USDA ARS Floriculture Nursery Research Initiative; Provost's Distinguished Graduate Fellowship - Oregon State University; National Science Foundation Graduate Research Fellowship [DGE-1314109]; National Science Foundation awards IOS [1340112, 1127112] FX This work was supported by the National Institute of Food and Agriculture, US Department of Agriculture award 2014-51181-22384 (JHC and NJG). Partial support also was provided by the USDA Agricultural Research Service Grant 5358-22000-039-00D (NJG), USDA National Institute of Food and Agriculture Grant 2011-68004-30154 (NJG), and the USDA ARS Floriculture Nursery Research Initiative (NJG). EWD is supported by a Provost's Distinguished Graduate Fellowship awarded by Oregon State University. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-1314109 to EWD. The summer camps were supported by National Science Foundation awards IOS #1340112 and #1127112 to Pankaj Jaiswal. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 100 TC 1 Z9 1 U1 5 U2 7 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD JUL 19 PY 2016 VL 4 AR e2222 DI 10.7717/peerj.2222 PG 27 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR7HK UT WOS:000380070400012 PM 27547538 ER PT J AU Wilson-Welder, JH Frank, AT Hornsby, RL Olsen, SC Alt, DP AF Wilson-Welder, Jennifer H. Frank, Ami T. Hornsby, Richard L. Olsen, Steven C. Alt, David P. TI Interaction of Bovine Peripheral Blood Polymorphonuclear Cells and Leptospira Species; Innate Responses in the Natural Bovine Reservoir Host SO FRONTIERS IN MICROBIOLOGY LA English DT Article DE Leptospira; serovar Hardjo; neutrophils; bovine host ID NEUTROPHIL EXTRACELLULAR TRAPS; IMMUNE-RESPONSE; GENE-EXPRESSION; GAMMA-DELTA; IN-VIVO; RECOGNITION; INFECTION; CATTLE; LIPOPOLYSACCHARIDE; INTERROGANS AB Cattle are the reservoir hosts of Leptospira borgpetersenii serovar Hardjo, and can also be reservoir hosts of other Leptospira species such as L. kirschneri, and Leptospira interrogans. As a reservoir host, cattle shed Leptospira, infecting other animals, including humans. Previous studies with human and murine neutrophils have shown activation of neutrophil extracellular trap or NET formation, and upregulation of inflammatory mediators by neutrophils in the presence of Leptospira. Humans, companion animals and most widely studied models of Leptospirosis are of acute infection, hallmarked by systemic inflammatory response, neutrophilia, and septicemia. In contrast, cattle exhibit chronic infection with few outward clinical signs aside from reproductive failure. Taking into consideration that there is host species variation in innate immunity, especially in pathogen recognition and response, the interaction of bovine peripheral blood polymorphonuclear cells (PMNs) and several Leptospira strains was evaluated. Studies including bovine-adapted strains, human pathogen strains, a saprophyte and inactivated organisms. Incubation of PMNs with Leptospira did induce slight activation of neutrophil NEIs, greater than unstimulated cells but less than the quantity from E. coli P4 stimulated PMNs. Very low but significant from non-stimulated, levels of reactive oxygen peroxides were produced in the presence of all Leptospira strains and E. coli P4. Similarly, significant levels of reactive nitrogen intermediaries (NO2) was produced from PMNs when incubated with the Leptospira strains and greater quantities in the presence of E. coil P4. PMNs incubated with Leptospira induced RNA transcripts of IL-1 beta, MIP-1 alpha, and TNF-alpha, with greater amounts induced by live organisms when compared to heat-inactivated leptospires. Transcript for inflammatory cytokine IL-8 was also induced, at similar levels regardless of Leptospira strain or viability. However, incubation of Leptospira strains with bovine PMNs did not affect Leptospira viability as measured by limiting dilution culture. This is in contrast to previously reported results of innate inflammatory activation by Leptospira in human and other animal models, or the activation and interaction of bovine PMNs with Escherichia coil and other bacterial pathogens. While it could be hypothesized that variations in innate receptor recognition, specifically variance in toll-like receptor 2, could underlie the observed reduction of activation in bovine PMNs. additional studies would be needed to explore this possibility. Reduction in neutrophil responses may help to establish nearly asymptomatic chronic Leptospira infection of cattle. This study emphasizes the importance of studying host-pathogen relationships in the appropriate species as extrapolation from other animal models may be incorrect and confounded by differences in the host responses. C1 [Wilson-Welder, Jennifer H.; Frank, Ami T.; Hornsby, Richard L.; Olsen, Steven C.; Alt, David P.] Natl Anim Dis Ctr, Infect Bacterial Dis Res Unit, Ames, IA 50010 USA. RP Wilson-Welder, JH (reprint author), Natl Anim Dis Ctr, Infect Bacterial Dis Res Unit, Ames, IA 50010 USA. EM jennifer.wilson-welder@ars.usda.gov NR 59 TC 0 Z9 0 U1 4 U2 4 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD JUL 19 PY 2016 VL 7 AR 1110 DI 10.3389/fmicb.2016.01110 PG 14 WC Microbiology SC Microbiology GA DR3ZF UT WOS:000379840500001 PM 27486445 ER PT J AU Khera, P Pandey, MK Wang, H Feng, SP Qiao, LX Culbreath, AK Kale, S Wang, JP Holbrook, CC Zhuang, WJ Varshney, RK Guo, BZ AF Khera, Pawan Pandey, Manish K. Wang, Hui Feng, Suping Qiao, Lixian Culbreath, Albert K. Kale, Sandip Wang, Jianping Holbrook, C. Corley Zhuang, Weijian Varshney, Rajeev K. Guo, Baozhu TI Mapping Quantitative Trait Loci of Resistance to Tomato Spotted Wilt Virus and Leaf Spots in a Recombinant Inbred Line Population of Peanut (Arachis hypogaea L.) from SunOleic 97R and NC94022 SO PLOS ONE LA English DT Article ID GENETIC-LINKAGE MAP; CULTIVATED PEANUT; RUST RESISTANCE; FIELD RESPONSE; OIL QUALITY; IDENTIFICATION; VARIETIES; SOFTWARE; GENOMICS; QTLS AB Peanut is vulnerable to a range of diseases, such as Tomato spotted wilt virus (TSWV) and leaf spots which will cause significant yield loss. The most sustainable, economical and eco-friendly solution for managing peanut diseases is development of improved cultivars with high level of resistance. We developed a recombinant inbred line population from the cross between SunOleic 97R and NC94022, named as the S-population. An improved genetic linkage map was developed for the S-population with 248 marker loci and a marker density of 5.7 cM/loci. This genetic map was also compared with the physical map of diploid progenitors of tetraploid peanut, resulting in an overall co-linearity of about 60% with the average co-linearity of 68% for the A sub-genome and 47% for the B sub-genome. The analysis using the improved genetic map and multi-season (2010-2013) phenotypic data resulted in the identification of 48 quantitative trait loci (QTLs) with phenotypic variance explained (PVE) from 3.88 to 29.14%. Of the 48 QTLs, six QTLs were identified for resistance to TSWV, 22 QTLs for early leaf spot (ELS) and 20 QTLs for late leaf spot (LLS), which included four, six, and six major QTLs ( PVE larger than 10%) for each disease, respectively. A total of six major genomic regions (MGR) were found to have QTLs controlling more than one disease resistance. The identified QTLs and resistance gene-rich MGRs will facilitate further discovery of resistance genes and development of molecular markers for these important diseases. C1 [Khera, Pawan; Wang, Hui; Guo, Baozhu] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Khera, Pawan; Pandey, Manish K.; Kale, Sandip; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Khera, Pawan; Wang, Hui; Culbreath, Albert K.; Guo, Baozhu] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Feng, Suping] Hainan Trop Marine Univ, Coll Trop Biol & Agron, Sanya, Peoples R China. [Qiao, Lixian] Qingdao Agr Univ, Coll Life Sci, Qingdao, Shandong, Peoples R China. [Wang, Jianping] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Holbrook, C. Corley] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Zhuang, Weijian] Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou, Peoples R China. RP Guo, BZ (reprint author), USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA.; Varshney, RK (reprint author), Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India.; Guo, BZ (reprint author), Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. EM r.k.varshney@cgiar.org; Baozhu.Guo@ars.usda.gov FU USDA Agricultural Research Service; Georgia Peanut Commission; Peanut Foundation; National Peanut Board of USA; World Bank assisted Watershed Development Project-II (KWDP-II) by Government of Karnataka, India FX This research was partially supported by funds provided by the USDA Agricultural Research Service, the Georgia Peanut Commission, Peanut Foundation and National Peanut Board of USA and World Bank assisted Watershed Development Project-II (KWDP-II) by Government of Karnataka, India. This work has been also undertaken as part of the CGIAR Research Program on Grain Legumes. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We thank Billy Wilson, Jake Fountain, Stephanie Lee, Lucero Gutierrez, Sara Beth Pelham, Victoria Weaver, and Jake Weaver for technical assistance in the field and the laboratory. This research was partially supported by funds provided by the USDA Agricultural Research Service, the Georgia Peanut Commission, Peanut Foundation and National Peanut Board of USA and World Bank assisted Watershed Development Project-II (KWDP-II) by Government of Karnataka, India. This work has been also undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 42 TC 2 Z9 2 U1 8 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 18 PY 2016 VL 11 IS 7 DI 10.1371/journal.pone.0158452 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR8TD UT WOS:000380169300007 ER PT J AU Nagy, A Xu, YF Bauchan, GR Shelton, DR Nou, XW AF Nagy, Attila Xu, Yunfeng Bauchan, Gary R. Shelton, Daniel R. Nou, Xiangwu TI Aggregative adherence fimbriae I (AAF/I) mediate colonization of fresh produce and abiotic surface by Shiga toxigenic enteroaggregative Escherichia coli O104:H4 SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Enteroaggregative E. coli; E. coli O104:H4; Aggregative adherence fimbriae; Fresh produce; Aggregation; Colonization; Biofilms ID HEMOLYTIC-UREMIC SYNDROME; BIOFILM FORMATION; MICROTITER-PLATE; OUTBREAK; O104/H4; GERMANY; ATTACHMENT; INFECTION; ADHESION; STRAINS AB The Shiga toxigenic Escherichia coli O104:H4 isolated during the 2011 European outbreak expresses Shiga toxin 2a and possess virulence genes associated with the enteroaggregative E. coli (EAEC) pathotype. It produces plasmid encoded aggregative adherence fimbriae I (AAF/I) which mediate cell aggregation and biofilm formation in human intestine and promote Shiga-toxin adsorption, but it is not clear whether the AAF/I fimbriae are involved in the colonization and biofilm formation on food and environmental matrices such as the surface of fresh produce. We deleted the gene encoding for the AAF/I fimbriae main subunit (AggA) from an outbreak associated E. coli O104:H4 strain, and evaluated the role of AAF/I fimbriae in the adherence and colonization of E. coli O104:H4 to spinach and abiotic surfaces. The deletion of aggA did not affect the adherence of E. coli O104:H4 to these surfaces. However, it severely diminished the colonization and biofilm formation of E. coli O104:H4 on these surfaces. Strong aggregation and biofilm formation on spinach and abiotic surfaces were observed with the wild type strain but not the isogenic aggA deletion mutant, suggesting that AAF/I fimbriae play a crucial role in persistence of O104:H4 cells outside of the intestines of host species, such as on the surface of fresh produce. Published by Elsevier B.V. C1 [Nagy, Attila; Xu, Yunfeng; Bauchan, Gary R.; Shelton, Daniel R.; Nou, Xiangwu] ARS, Environm Microbial & Food Safety Lab, Electron & Confocal Microscopy Unit, USDA, Beltsville, MD 20705 USA. [Nagy, Attila] NIAID, NIH, Vaccine Res Ctr, Gaithersburg, MD 20878 USA. RP Nou, XW (reprint author), ARS, Environm Microbial & Food Safety Lab, Electron & Confocal Microscopy Unit, USDA, Beltsville, MD 20705 USA. EM xiangwu.nou@ars.usda.gov FU China Scholarship Council FX The authors thank Michigan State University EHEC Stock Center for providing E. coli O104:H4 strain TW16133, and Yale University E. coli Genetic Stock Center for providing pKD78 used in this study. Y. Xu is a pre-doctoral visiting student from Northwest A&F University, China, and is supported by China Scholarship Council. NR 25 TC 3 Z9 3 U1 2 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JUL 16 PY 2016 VL 229 BP 44 EP 51 DI 10.1016/j.ijfoodmicro.2016.04.007 PG 8 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA DO6XR UT WOS:000377927300006 PM 27099984 ER PT J AU Schaut, RG Ridpath, JF Sacco, RE AF Schaut, Robert G. Ridpath, Julia F. Sacco, Randy E. TI Bovine Viral Diarrhea Virus Type 2 Impairs Macrophage Responsiveness to Toll-Like Receptor Ligation with the Exception of Toll-Like Receptor 7 SO PLOS ONE LA English DT Article ID BLOOD MONONUCLEAR-CELLS; HEPATITIS-C VIRUS; INTERFERON REGULATORY FACTOR-3; MESSENGER-RNA EXPRESSION; IN-VITRO; PROTEASOMAL DEGRADATION; MUCOSAL DISEASE; ACUTE INFECTION; T-CELLS; BVDV AB Bovine viral diarrhea virus (BVDV) is a member of the Flaviviridae family. BVDV isolates are classified into two biotypes based on the development of cytopathic (cp) or non-cytopathic (ncp) effects in epithelial cell culture. BVDV isolates are further separated into species, BVDV1 and 2, based on genetic differences. Symptoms of BVDV infection range from subclinical to severe, depending on strain virulence, and may involve multiple organ systems and induction of a generalized immunosuppression. During BVDV-induced immune suppression, macrophages, critical to innate immunity, may have altered pathogen recognition receptor (PRR) signaling, including signaling through toll-like receptors (TLRs). Comparison of BVDV 2 strains with different biotypes and virulence levels is valuable to determining if there are differences in host macrophage cellular responses between viral phenotypes. The current study demonstrates that cytopathic (cp), noncytopathic (ncp), high (hv) or low virulence (lv) BVDV2 infection of bovine monocyte-derived macrophages (MDMF) result in differential expression of pro-inflammatory cytokines compared to uninfected MDMF. A hallmark of cp BVDV2 infection is IL-6 production. In response to TLR2 or 4 ligation, as might be observed during secondary bacterial infection, cytokine secretion was markedly decreased in BVDV2-infected MDMF, compared to non-infected MDMF. Macrophages were hyporesponsive to viral TLR3 or TLR8 ligation. However, TLR7 stimulation of BVDV2-infected MDMF induced cytokine secretion, unlike results observed for other TLRs. Together, these data suggest that BVDV2 infection modulated mRNA responses and induced a suppression of proinflammatory cytokine protein responses to TLR ligation in MDMF with the exception of TLR7 ligation. It is likely that there are distinct differences in TLR pathways modulated following BVDV2 infection, which have implications for macrophage responses to secondary infections. C1 [Schaut, Robert G.; Sacco, Randy E.] Iowa State Univ, Immunobiol Grad Program, Ames, IA 50011 USA. [Schaut, Robert G.; Ridpath, Julia F.; Sacco, Randy E.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Sacco, RE (reprint author), Iowa State Univ, Immunobiol Grad Program, Ames, IA 50011 USA.; Sacco, RE (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM randy.sacco@ars.usda.gov NR 69 TC 0 Z9 0 U1 4 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 15 PY 2016 VL 11 IS 7 AR e0159491 DI 10.1371/journal.pone.0159491 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR1JR UT WOS:000379662300014 PM 27420479 ER PT J AU del Cacho, E Gallego, M Lillehoj, HS Quilez, J Lillehoj, EP Sanchez-Acedo, C AF del Cacho, Emilio Gallego, Margarita Lillehoj, Hyun Soon Quilez, Joaquin Lillehoj, Erik P. Sanchez-Acedo, Caridad TI Induction of protective immunity against experimental Eimeria tenella infection using serum exosomes SO VETERINARY PARASITOLOGY LA English DT Article DE Chicken; Dendritic cell; Serum exosome; Eimeria tenella; Immunity ID CELL-DERIVED EXOSOMES; FOLLICULAR DENDRITIC CELLS; ANTICOCCIDIAL DRUGS; IN-VIVO; VESICLES; VACCINE; COCCIDIOSIS; MICROVESICLES; RESPONSES; CHICKENS AB Avian coccidiosis is caused by Eimeria, a unicellular, apicomplexan protist which primarily infects intestinal epithelia resulting in nutrient malabsorption and reduced growth of commercial poultry. Vaccination of chickens with exosomes isolated from antigen presenting cells containing parasite antigens (Ags) represents a promising alternative strategy to control avian coccidiosis, but is restricted in its commercial application due to limitations on production scale-up for mass immunization programs. Here, we report the biochemical and physiologic characteristics of exosomes derived from serum of Eimeria tenella-infected chickens and their feasibility for inducing protective immunity to experimental coccidiosis. Exosomes isolated from the serum of E. tenella-infected chickens contained a subset of protein Ags found in the intact parasite. Serum-derived exosomes containing these E. tenella Ags localized to the intestine and spleen following intramuscular injection into naive chickens. In vitro ELISPOT assays revealed increased numbers of IL-2-, IL-4-, IL-6-, and IFN-gamma-secreting cells in the intestine and spleen of exosome-administered chickens, compared with vehicle controls. Pre-immunization of chickens with serum exosomes from E. tenella-infected chickens increased both body weight gain and feed conversion efficiency, and reduced both fecal parasite shedding and gut lesion scores following parasite infection, compared with vehicle controls. Finally, immunization with CD80(+) serum exosomes stimulated greater numbers of cytokine-producing cells, and higher levels of protective immunity to E. tenella infection, compared with CD80(-) exosomes. These results suggest the possibility of producing an effective, parasite-free vaccine against avian coccidiosis under field conditions using serum-derived CD80(+) exosomes containing parasite Ags. (C) 2016 Elsevier B.V. All rights reserved. C1 [del Cacho, Emilio; Gallego, Margarita; Quilez, Joaquin; Sanchez-Acedo, Caridad] Univ Zaragoza, Dept Anim Pathol, Fac Vet Sci, Zaragoza, Spain. [Lillehoj, Hyun Soon] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Lillehoj, Erik P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. RP del Cacho, E (reprint author), Fac Vet, Parasitol & Enfermedades Parasitarias, Miguel Servet 177, Zaragoza 50013, Spain. EM edelcach@unizar.es FU Research Council of Aragon (Spain) [A46]; USDA-CSREES [2005-01812] FX This investigation was partially funded by Grant A46 from the Research Council of Aragon (Spain) and USDA-CSREES proposal 2005-01812 (US Veterinary Immune Reagent Network). NR 35 TC 1 Z9 1 U1 5 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 15 PY 2016 VL 224 BP 1 EP 6 DI 10.1016/j.vetpar.2016.04.043 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DQ1KO UT WOS:000378960100001 PM 27270382 ER PT J AU Djemai, S Mekroud, A Jenkins, MC AF Djemai, Samir Mekroud, Abdeslam Jenkins, Mark C. TI Evaluation of ionophore sensitivity of Eimeria acervulina and Eimeria maxima isolated from the Algerian to Jijel province poultry farms SO VETERINARY PARASITOLOGY LA English DT Article DE Ionophore; Eimeria; Internal transcribed spacer 1 PCR; Anticoccidial index ID ANTICOCCIDIAL DRUGS; BROILER OPERATIONS; AVIAN EIMERIA; RESISTANCE; COCCIDIOSIS; MONENSIN; TENELLA; CHICKENS; STRATEGIES; FOWL AB The present study represents the first description of ionophore resistance in recovered from commercial Algerian (Jijel-Algeria) broiler farms. Microscopy and intervening transcribed sequence 1 PCR (ITS1 PCR) revealed only 2 Eimeria species present in litter from these farms- namely Eimeria acervulina and Eimeria maxima. A pool of these isolates were evaluated in broiler chickens (Cobb 500) for sensitivity to 5 anticoccidial compounds-diclazuril (1 ppm), lasalocid (125 ppm), monensin (125 ppm), narasin (70 ppm) and salinomycin (60 ppm). As indicated by anticoccidial sensitivity profiles based on lesion scores and anticoccidial index (ACI), complete resistance to monensin and narasin, partial resistance to salinomycin and lasalocid, and complete sensitivity to diclazuril was observed. While lack of sensitivity to monensin is not surprising given its use for years as the sole anticoccidial compound, the resistance to monoether (narasin) and polyether (lasalocid) ionophores suggests that cross-resistance has developed in a segment of the Eimeria population. The fairly uniform Eimeria species composition among all poultry farms suggests that E. acervulina and E. maxima more rapidly develop resistance to ionophore drugs. (C) 2016 Elsevier B.V. All rights reserved. C1 [Djemai, Samir; Mekroud, Abdeslam] Univ Constantine, Lab, PADESCA, Constantine, Algeria. [Jenkins, Mark C.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Djemai, S (reprint author), Univ Mentouri Constantine, PADESCA, Inst Sci Vet Elkhroub, Lab, Constantine, Algeria. EM djemaiamersamir@gmail.com; mark.jenkins@ars.usda.gov NR 35 TC 0 Z9 0 U1 6 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 15 PY 2016 VL 224 BP 77 EP 81 DI 10.1016/j.vetpar.2016.04.040 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA DQ1KO UT WOS:000378960100013 PM 27270394 ER PT J AU Nichols, MH Nearing, M Hernandez, M Polyakov, VO AF Nichols, M. H. Nearing, M. Hernandez, M. Polyakov, V. O. TI Monitoring channel head erosion processes in response to an artificially induced abrupt base level change using time-lapse photography SO GEOMORPHOLOGY LA English DT Article DE Gully erosion; Piping; Subsurface erosion; Time-lapse photography ID SOUTHEASTERN ARIZONA; SEMIARID WATERSHEDS; RETREAT RATES; MIGRATION; PLATEAU; SYSTEM; USA AB Gullies that terminate at a vertical-wall are ubiquitous throughout arid and semiarid regions. Multi-year assessments of gully evolution and headcut advance are typically accomplished using traditional ground surveys and aerial photographs, with much recent research focused on integrating data collected at very high spatial resolutions using new techniques such as aerial surveys with blimps or kites and ground surveys with LiDar scanners. However, knowledge of specific processes that drive headcut advance is limited due to inadequate observation and documentation of flash floods and subsequent erosion that can occur at temporal resolutions not captured through repeat surveys. This paper presents a method for using very-high temporal resolution ground-based time-lapse photography to capture short-duration flash floods and gully head evolution in response. In 2004, a base level controlling concrete weir was removed from the outlet of a 129 ha semiarid headwater drainage on the Walnut Gulch Experimental Watershed in southeastern Arizona, USA. During the ten year period from 2004 to 2014 the headcut migrated upchannel a total of 14.5 m reducing the contributing area at the headwall by 9.5%. Beginning in July 2012, time-lapse photography was employed to observe event scale channel evolution dynamics. The most frequent erosion processes observed during three seasons of time-lapse photography were plunge pool erosion and mass wasting through sidewall or channel headwall slumping that occurred during summer months. Geomorphic change during the ten year period was dominated by a single piping event in August 2014 that advanced the channel head 7.4 m (51% of the overall advance) and removed 11.3 m(3) of sediment. High temporal resolution time-lapse photography was critical for identifying subsurface erosion processes, in the absence of time-lapse images piping would not have been identified as an erosion mechanism responsible for advancing the gully headwall at this site. Published by Elsevier B.V. C1 [Nichols, M. H.; Nearing, M.; Hernandez, M.; Polyakov, V. O.] USDA, ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. RP Nichols, MH (reprint author), USDA, ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM mary.nichols@ars.usda.gov NR 56 TC 0 Z9 0 U1 14 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X EI 1872-695X J9 GEOMORPHOLOGY JI Geomorphology PD JUL 15 PY 2016 VL 265 BP 107 EP 116 DI 10.1016/j.geomorph.2016.05.001 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA DP0MD UT WOS:000378183000008 ER PT J AU Finer, L Jurgensen, M Palviainen, M Piirainen, S Page-Dumroese, D AF Finer, L. Jurgensen, M. Palviainen, M. Piirainen, S. Page-Dumroese, D. TI Does clear-cut harvesting accelerate initial wood decomposition? A five-year study with standard wood material SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Coarse woody debris; Decay; Nitrogen; Pinus sylvestris; Pinus taeda; Populus tremuloides; Soil preparation; Soil temperature ID BOREAL FORESTS; CONIFEROUS FORESTS; NORWAY SPRUCE; BIRCH STEMS; DECAY-RATES; FIR FOREST; SCOTS PINE; DEBRIS; NITROGEN; SOIL AB Coarse woody debris (CWD) serves a variety of ecological functions in forests, and the understanding of its decomposition is needed for estimating changes in CWD-dependent forest biodiversity, and for the quantification of forest ecosystem carbon and nutrient pools and fluxes. Boreal forests are often intensively managed, so information is needed on the effects of timber harvesting on wood decomposition, and the factors controlling the decomposition process. Therefore, decomposition of standard wood stakes of Scots pine, loblolly pine, and aspen were monitored in an uncut forest and in an adjacent clear-cut in Finland. Stakes of each species were placed horizontally on the top of the surface organic layer, at the organic layer-mineral soil interface, and vertically in the mineral soil to depth of 20 cm in both the uncut forest and in the clear-cut. Five stakes of each tree species were taken every year from each stake location for five years. Mass loss of wood stakes from all three species was greater in the clear-cut than in the uncut forest during the five-year decomposition period, losing an average 59.8% of their mass in the clear-cut, which was greater than mass loss by both pines (19.8 +/- 3.0SE%) and aspen (43.3 +/- 5.1SE%) in the uncut forest. Aspen wood stakes decomposed faster than both Scots and loblolly pine stakes in the uncut forest during the whole study period, but after two years there were no differences between the three species in the clear-cut. In the uncut forest, mass loss of stakes on the surface of the organic layer was 6-10% faster than those at the mineral soil interface or in the mineral soil. In contrast, mass loss of stakes, placed on the top of organic layer in the clear-cut was 32-35% lower than those deeper in the soil probably due to low moisture conditions at the soil surface. Wood stake mass loss was positively correlated with the sum of soil temperature degree days (r >= 0.94). In the uncut forest mass loss was positively correlated with wood stake N accumulation, indicating that N availability was also a factor in decomposition before harvesting. Our study indicates that wood decomposition in this boreal forest is more sensitive to increased soil temperatures and N availability after clear-cut harvesting than found in earlier studies. (C) 2016 Elsevier B.V. All rights reserved. C1 [Finer, L.; Piirainen, S.] Nat Resources Inst Finland, Box 68, FI-80101 Joensuu, Finland. [Jurgensen, M.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. [Palviainen, M.] Univ Helsinki, Dept Forest Sci, Box 27, FIN-00014 Helsinki, Finland. [Page-Dumroese, D.] US Forest Serv, USDA, Rocky Mt Res Stn, Forestry Sci Lab, 1221 S Main St, Moscow, ID 83843 USA. RP Finer, L (reprint author), Nat Resources Inst Finland, Box 68, FI-80101 Joensuu, Finland. EM leena.finer@luke.fi; mfjurgen@mtu.edu; sirpa.piirainen@luke.fi; ddumroese@fs.fed.us OI Finer, Leena/0000-0001-7623-9374 NR 50 TC 0 Z9 0 U1 11 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2016 VL 372 BP 10 EP 18 DI 10.1016/j.foreco.2016.03.060 PG 9 WC Forestry SC Forestry GA DN1BZ UT WOS:000376802600002 ER PT J AU Tavernia, BG Nelson, MD Garner, JD Perry, CH AF Tavernia, Brian G. Nelson, Mark D. Garner, James D. Perry, Charles H. TI Spatial characteristics of early successional habitat across the Upper Great Lakes states SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Early successional forest; American Woodcock; Young forest; Habitat assessment; Canopy disturbance; Landscape metrics ID NORTHEASTERN UNITED-STATES; NORTH-AMERICAN FORESTS; SHRUBLAND BIRDS; DISTURBANCE; ABUNDANCE; PATTERNS; CONSERVATION; VARIABILITY; SENSITIVITY; CLEARCUTS AB Creation and management of early successional forest (ESF) is needed to halt and reverse declines of bird species dependent on pioneering plant species or young forests. ESF-dependent bird species require specific structural forest classes and are sensitive to forest age (a surrogate for forest structure), patch size, proximity to patch edges, and the juxtaposition of forest age classes. To date, ESF conservation plans have relied on spatially inexplicit data, lacking patch and landscape metrics, to set habitat goals and to track habitat trends. In a previous study, we used Landsat time series stacks and a vegetation-change tracker algorithm to track forest canopy disturbances and subsequent regrowth from 1990 to 2009 across the Upper Great Lakes Young Forest Initiative region. Based on canopy disturbance histories, we assigned forest age classes to forest classes of the National Land Cover Database of 2011. In the present study, we used this spatial product to assess areas, patch and edge metrics, and land protection statuses of deciduous-mixed forest and woody wetland age classes. We defined ESF using four 5-year-age classes (1-5, 6-10, 11-15, 16-20 years old) and their aggregate (1-20 years old) whereas forest >20 years old was referred to as 'persisting'. Aggregated across 5-year-age classes, ESF of deciduous-mixed forest covered 3.4% and 0.9% of Bird Conservation Regions (BCR) 12 (Boreal Hardwood Transition) and 23 (Prairie Hardwood Transition), respectively, whereas woody wetland ESF constituted 1.0% and 0.2% of the same BCRs. For both deciduous-mixed forest and woody wetlands, ESF often occurred in patches >= 1 ha, but most ESF also occurred near patch edges created by adjacencies with persisting forest. Most ESF tell on lands with an unprotected or unknown protection status regardless of forest class. Regionally, ESF covered less area, occurred in smaller patches and nearer to edges, and more often fell on lands of unprotected or unknown protection status in BCR 23 than in BCR 12. Our results advance ESF conservation by providing insight into spatial characteristics that influence habitat quality and by establishing a baseline for habitat management planning and monitoring. (C) 2016 Elsevier B.V. All rights reserved. C1 [Tavernia, Brian G.] Nature Conservancy, Colorado Field Off, 2424 Spruce St, Boulder, CO 80302 USA. [Nelson, Mark D.; Garner, James D.; Perry, Charles H.] US Forest Serv, USDA, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. RP Tavernia, BG (reprint author), Nature Conservancy, Colorado Field Off, 2424 Spruce St, Boulder, CO 80302 USA. EM btavernia@gmail.com; mdnelson@fs.fed.us; jamesdgarner@fs.fed.us; charleshperry@fs.fed.us OI Perry, Charles/0000-0001-9377-5996 FU U.S. Fish and Wildlife Service's Webless Migratory Game Bird Program FX We thank the U.S. Fish and Wildlife Service's Webless Migratory Game Bird Program for funding. The University of Missouri, U.S. Forest Service's Northern Research Station, and U.S. Geological Survey's Patuxent Wildlife Research Center administratively supported the first author during creation and validation of the early successional forest map used for this habitat assessment. We thank David Andersen, Tom Cooper, John Sanderson, John Stanovick and two anonymous reviewers for comments that improved an earlier version of the manuscript. Mention of any specific commercial products, process, or service by trade name, trademark, or manufacturer does not constitute or imply its endorsement, recommendation, or favoring by the United States Government. NR 62 TC 0 Z9 0 U1 10 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2016 VL 372 BP 164 EP 174 DI 10.1016/j.foreco.2016.04.003 PG 11 WC Forestry SC Forestry GA DN1BZ UT WOS:000376802600018 ER PT J AU Clough, BJ Russell, MB Domke, GM Woodall, CW AF Clough, Brian J. Russell, Matthew B. Domke, Grant M. Woodall, Christopher W. TI Quantifying allometric model uncertainty for plot-level live tree biomass stocks with a data-driven, hierarchical framework SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest biomass; National greenhouse gas inventory; Data assimilation; Bayesian hierarchical models ID DATA ASSIMILATION; NATIONAL FOREST; CENTRAL-EUROPE; CARBON; PREDICTIONS; DIAMETER; EQUATIONS; PATTERNS; HEIGHT; VOLUME AB Accurate uncertainty assessments of plot-level live tree biomass stocks are an important precursor to estimating uncertainty in annual national greenhouse gas inventories (NGHGIs) developed from forest inventory data. However, current approaches employed within the United States' NGHGI do not specifically incorporate methods to address error in tree-scale biomass models and as a result may misestimate overall uncertainty surrounding plot-scale assessments. We present a data-driven, hierarchical modeling approach to predict both total aboveground and foliage biomass for inventory plots within the US Forest Service Forest Inventory and Analysis (FIA) program, informed by a large multispecies felled-tree dataset. Our results reveal substantial plot-scale relative uncertainties for total aboveground biomass (11-155% of predicted means) with even larger uncertainties for foliage biomass (27-472%). In addition, we found different distributions of total aboveground and foliage biomass when compared with other generalized biomass models for North America. These results suggest a greater contribution of allometric models to the overall uncertainty of biomass stock estimates than what has been previously reported by the literature. While the relative performance of the hierarchical model is influenced by biases within the fitting data, particularly for woodland and conifer species, our results suggest that poor representation of individual tree model error may lead to unrealistically high confidence in plot-scale estimates of biomass stocks derived from forest inventory data. However, improvements to model design and the quality of felled-tree data for fitting and validation may offer substantial improvements in the accuracy and precision of NGHGIs. (C) 2016 Elsevier B.V. All rights reserved. C1 [Clough, Brian J.; Russell, Matthew B.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Domke, Grant M.; Woodall, Christopher W.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. RP Clough, BJ (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN USA. EM bclough@umn.edu OI Domke, Grant/0000-0003-0485-0355 FU US Forest Service Northern Research Station; Minnesota Agricultural Experiment Station FX We wish to thank the compilers and contributors of the legacy biomass data used in our study, particularly Phil Radtke, Aaron Weiskittel, David Walker, and Jereme Frank. In addition, we thank Johannes Breidenbach, David Bell, Ram Deo, and two anonymous reviewers for commenting on this manuscript. This work is supported by the US Forest Service Northern Research Station and the Minnesota Agricultural Experiment Station. NR 61 TC 0 Z9 0 U1 7 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2016 VL 372 BP 175 EP 188 DI 10.1016/j.foreco.2016.04.001 PG 14 WC Forestry SC Forestry GA DN1BZ UT WOS:000376802600019 ER PT J AU Hutchinson, TF Rebbeck, J Stout, SL AF Hutchinson, Todd F. Rebbeck, Joanne Stout, Susan L. TI The devil is in the small dense saplings: A midstory herbicide treatment has limited effects on short-term regeneration outcomes in oak shelterwood stands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Silviculture; Quercus; Regeneration; Shelterwood; Competition ID REPEATED PRESCRIBED FIRES; ADVANCE REPRODUCTION; FORESTS AB On intermediate quality sites, where oak advance regeneration often accumulates, we tested whether a low-intensity herbicide treatment of shade-tolerant saplings and poles (injection of stems >5 cm DBH with glyphosate), conducted just prior to a shelterwood harvest, could increase the proportion of oak (and hickory) in the regeneration layer after the harvest. Control and herbicide units were established at four study sites in southern Ohio. Advance reproduction was measured before and 4-6 years after a shelterwood harvest that reduced basal area by 50%. Before the harvest, shade-tolerant species, mainly red maple, blackgum, and sourwood, dominated the sapling layer but established oak-hickory seedlings were present at moderate densities. After the harvest, the proportion of oak-hickory did not change significantly on either control or herbicide units and non-oaks were dominant in the majority of plots. However, larger oak-hickory regeneration (>70 cm height) developed on nearly 50% of the sampling units (2-m radius subplots) and oak-hickory regeneration was dominant on a greater proportion of subplots in the herbicide units (26%) than in the control units (13%). Herbicide effects were limited due to the large number of smaller non-oak stems (<5 cm DBH) that were not treated and also the ineffectiveness of glyphosate to prevent red maple stump sprouting. The heavy shelterwood first removal cut stimulated the growth of both oak seedlings and competing stems, and the herbicide treatment resulted in very limited improvements in the competitive position of the oaks. However, because the oaks did survive and grow, additional treatments may still change the outcome on these sites. Published by Elsevier B.V. C1 [Hutchinson, Todd F.; Rebbeck, Joanne] US Forest Serv, USDA, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. [Stout, Susan L.] US Forest Serv, USDA, No Res Stn, 335 Natl Forge Rd,POB 267, Irvine, PA 16329 USA. RP Hutchinson, TF (reprint author), US Forest Serv, USDA, No Res Stn, 359 Main Rd, Delaware, OH 43015 USA. EM thutchinson@fs.fed.us FU USDA Forest Service, Northern Research Station FX We thank the Ohio Department of Natural Resources, Division of Forestry for providing the study sites, and marking and implementing the shelterwood harvests. We thank David Hosack, William Borovicka, David Runkle, and Levi Miller for leading the field work effort throughout the study. Aaron Iverson and Wynn Johnson collected the pre-treatment data. Also, a cadre of students from Hocking Technical College, Ohio University, and Ohio State University contributed to posttreatment data collection. We thank John Kabrick, Tom Schuler, Patrick Brose, and Gary Miller for reviewing earlier drafts of this manuscript. Two anonymous reviewers provided useful comments on the original submission. John Stanovick provided statistical support and a biometrics review of the manuscript. Funding was provided by the USDA Forest Service, Northern Research Station. NR 35 TC 0 Z9 0 U1 10 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2016 VL 372 BP 189 EP 198 DI 10.1016/j.foreco.2016.04.016 PG 10 WC Forestry SC Forestry GA DN1BZ UT WOS:000376802600020 ER PT J AU Burton, JI Olson, DH Puettmann, KJ AF Burton, Julia I. Olson, Deanna H. Puettmann, Klaus J. TI Effects of riparian buffer width on wood loading in headwater streams after repeated forest thinning SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Best management practices; Coarse woody debris; Density management; Forest management; Pacific Northwest; Stream management zone ID DOUGLAS-FIR FORESTS; OREGON COAST RANGE; WESTERN OREGON; OLD-GROWTH; PACIFIC-NORTHWEST; CLEAR-CUT; CONIFEROUS STANDS; INSTREAM WOOD; DEBRIS; USA AB Forested riparian buffer zones are used in conjunction with upland forest management, in part, to provide for the recruitment for large wood to streams. Small headwater streams account for the majority of stream networks in many forested regions. Yet, our understanding of how riparian buffer width influences wood dynamics in headwater streams is relatively less developed compared to larger fish-bearing streams. The effects of riparian buffer width on instream wood loading after thinning can be difficult to discern due to the influence of basin characteristics and reach-scale geomorphology on wood recruitment, breakage and redistribution. We assessed the relationships between instream wood loading, geomorphology and riparian buffer width in small headwater streams after upland thinning. Then we examined the distances between pieces of stream wood and their sources, or the distance from which wood volumes were recruited to these streams. Data were collected along 34 stream reaches at six different sites in a replicated field experiment, comparing three no-harvest streamside buffer treatments (similar to 6-m, 15-m minimum, and similar to 70-m widths). At each site, second-growth forests were thinned first to 200 trees per ha [tph] and similar to 10 years later to 85 tph, alongside an unthinned reference unit (similar to 400 tph). We measured wood loading (m(3)/100 m) four times: (1) prior to thinning; (2) year 5 post-1st thinning; (3) immediately prior to the 2nd thinning; and (4) year 1 post-2nd thinning. The majority of wood volume was in late stages of decay, most likely biological legacies from the previous forest stand, and distributed along the streambank. Surprisingly, wood volume in early stages of decay was higher in stream reaches with a narrow 6-m buffer than in stream reaches with larger 15- and 70-m buffers and the unthinned reference units. Additionally, wood volume increased with drainage basin area. Only 45% of wood in late stages of decay could be associated with a particular source. Yet, 82% and 85% of sourced wood in early and late stages of decay, respectively, originated from within 15 m of streams. Expected continue low rates will likely result in declining volumes of wood in late stages of decay. Thinning and directional felling of logs into to streams could be used to augment wood volumes in the near term, and accelerate the development of large-diameter logs for future inputs. However, the relationship between instream wood loading and basin area suggests that instream wood loading depends on management across the entire watershed. (C) 2016 Elsevier B.V. All rights reserved. C1 [Burton, Julia I.; Puettmann, Klaus J.] Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. [Olson, Deanna H.] US Forest Serv, Pacific NW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. RP Burton, JI (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, 321 Richardson Hall, Corvallis, OR 97331 USA. EM julia.burton@oregonstate.edu; dedeolson@fs.fed.us RI Burton, Julia/C-8395-2009 OI Burton, Julia/0000-0002-3205-8819 FU U.S. Forest Service Pacific Northwest Research Station; American Recovery and Reinvestment Act; Bureau of Land Management; Oregon State University FX We thank Loretta Ellenburg for overseeing all field data collection over the full span of this study, Kelly Christiansen for GIS assistance, Adrian Ares for early insights into data management and analyses, Kathryn Ronnenberg for editorial and graphical assistance and Ariel Muldoon for statistical consultation. Support was provided by the U.S. Forest Service Pacific Northwest Research Station, the American Recovery and Reinvestment Act, Bureau of Land Management, and Oregon State University. Louisa Evers, Gordon Reeves and three anonymous reviewers provided constructive feedback on the original version leading to significant improvements. NR 84 TC 1 Z9 1 U1 12 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 15 PY 2016 VL 372 BP 247 EP 257 DI 10.1016/j.foreco.2016.03.053 PG 11 WC Forestry SC Forestry GA DN1BZ UT WOS:000376802600026 ER PT J AU Kern, RJ Lindholm-Perry, AK Freetly, HC Snelling, WM Kern, JW Keele, JW Miles, JR Foote, AP Oliver, WT Kuehn, LA Ludden, PA AF Kern, Rebecca J. Lindholm-Perry, Amanda K. Freetly, Harvey C. Snelling, Warren M. Kern, John W. Keele, John W. Miles, Jeremy R. Foote, Andrew P. Oliver, William T. Kuehn, Larry A. Ludden, Paul A. TI Transcriptome differences in the rumen of beef steers with variation in feed intake and gain SO GENE LA English DT Article DE Beef cattle; RNA-Seq; Rumen; Gain; Feed intake ID GENE-EXPRESSION; TIGHT JUNCTIONS; SODIUM-BUTYRATE; DAIRY-COWS; CATTLE; EFFICIENCY; TRANSPORT; TISSUE; FORESTOMACH; DISEASES AB Background: Feed intake and gain are economically important traits in beef production. The rumen wall interacts with feed, microbial populations, and fermentation products important to cattle nutrition. As such, it is likely to be a critical component in the beef steer's ability to utilize feedstuffs efficiently. To identify genes associated with steer feed intake and body weight gain traits, and to gain an understanding of molecules and pathways involved in feed intake and utilization, RNA sequencing (RNA-Seq) was performed on rumen papillae from 16 steers with variation in gain and feed intake. Four steers were chosen from each of the four Cartesian quadrants for gain x feed intake and used to generate individual RNA-Seq libraries. Results: Normalized read counts from all of the mapped reads from each of the four groups of animals were individually compared to the other three groups. In addition, differentially expressed genes (DEGs) between animals with high and low gain, as well as high and low intake were also evaluated. A total of 931 genes were differentially expressed in the analyses of the individual groups. Eighty-nine genes were differentially expressed between high and low gain animals; and sixty-nine were differentially expressed in high versus low intake animals. Several of the genes identified in this study have been previously associated with feed efficiency. Among those are KLK10, IRX3, COL1A1, CRELD2, HDAC10, IFITM3, and VIM. Conclusions: Many of the genes identified in this study are involved with immune function, inflammation, apoptosis, cell growth/proliferation, nutrient transport, and metabolic pathways and may be important predictors of feed intake and gain in beef cattle. Published by Elsevier B.V. C1 [Keele, John W.; Ludden, Paul A.] Univ Wyoming, Dept Anim Sci, Laramie, WY 82070 USA. [Kern, Rebecca J.; Lindholm-Perry, Amanda K.; Freetly, Harvey C.; Snelling, Warren M.; Miles, Jeremy R.; Foote, Andrew P.; Oliver, William T.; Kuehn, Larry A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Kern, John W.] Kern Stat Serv, Sauk Rapids, MN 56379 USA. RP Lindholm-Perry, AK (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Rebecca.Kern@ARS.USDA.GOV; Amanda.Lindholm@ARS.USDA.GOV; Harvey.Freetly@ARS.USDA.GOV; Warren.Snelling@ARS.USDA.GOV; jkern@kernstat.com; john.keele@ars.usda.gov; Jeremy.Miles@ARS.USDA.GOV; Andrew.Foote@ars.usda.gov; William.Oliver@ars.usda.gov; Larry.Kuehn@ars.usda.gov; Ludden@uwyo.edu NR 53 TC 1 Z9 1 U1 7 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD JUL 15 PY 2016 VL 586 IS 1 BP 12 EP 26 DI 10.1016/j.gene.2016.03.034 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA DM9OQ UT WOS:000376696000003 PM 27033587 ER PT J AU Deiss, L de Moraes, A Dieckow, J Franzluebbers, AJ Gatiboni, LC Sassaki, GL Carvalho, PCF AF Deiss, Leonardo de Moraes, Anibal Dieckow, Jeferson Franzluebbers, Alan J. Gatiboni, Luciano Colpo Sassaki, Guilherme Lanzi Carvalho, Paulo C. F. TI Soil phosphorus compounds in integrated crop-livestock systems of subtropical Brazil SO GEODERMA LA English DT Article DE Global phosphorus security; Sustainable intensification; Food security; Environmental quality; Land-use; Mixed crop-livestock ID NUCLEAR-MAGNETIC-RESONANCE; P-31 NMR-SPECTROSCOPY; ORGANIC PHOSPHORUS; GRAZING INTENSITIES; SOYBEAN YIELD; FORMS; LAMBS; IDENTIFICATION; AVAILABILITY; FERTILITY AB Soil phosphorus (P) utilization may be affected by agricultural complexity, in particular when combining annual crops and livestock grazing on the same land area and at overlapping times. Our objectives were to qualify and quantify soil organic and inorganic P compounds using sodium hydroxide-ethylenediaminetetraacetic acid (NaOH-EDTA) extraction and P-31 nuclear magnetic resonance spectroscopy (P-31 NMR) in response to increasing complexity with integrated crop-livestock systems (ICLS) in subtropical Brazil. Soil at a depth of 0-5 cm was collected from three long-term (7 to 12 years) cropping studies with and without ruminant grazing of cover crops. All sites were managed under no tillage, and treatments with livestock were managed with moderate grazing intensity. In these agro-ecosystems, grazing compared with no-grazing had greater soil P content as total and bioavailable orthophosphate and lower soil organic P and fewer monoesters, including inositol phosphates. Grazing increased P bioavailability and reduced recalcitrant organic P concentration in soil; therefore, cropping systems that integrate livestock (ICLS) can be a sustainable alternative to improve P use in farming systems of subtropical Brazil. (C) 2016 Elsevier B.V. All rights reserved. C1 [Deiss, Leonardo; de Moraes, Anibal] Fed Univ Parana UFPR, Agr Sci Sect, Dept Crop Prod & Protect, Rua Funcionarios 1-540, BR-80035050 Curitiba, Parana, Brazil. [Dieckow, Jeferson] Fed Univ Parana UFPR, Dept Soil Sci & Agr Engn, Rua Funcionarios 1540, BR-80035050 Curitiba, Parana, Brazil. [Franzluebbers, Alan J.] USDA ARS, 3218 Williams Hall,NCSU Campus Box 7619, Raleigh, NC 27695 USA. [Gatiboni, Luciano Colpo] Santa Catarina State Univ UDESC, Ave Luis de Camoes 2090, BR-88520000 Lages, SC, Brazil. [Sassaki, Guilherme Lanzi] Fed Univ Parana UFPR, Biol Sci Sect, Dept Biochem, Coronel Francisco H dos Santos Ave, BR-19031 Curitiba, Parana, Brazil. [Carvalho, Paulo C. F.] Univ Fed Rio Grande do Sul, Fac Agron, Ave Bento Goncalves 7712,Cx Postal 776, BR-91501970 Porto Alegre, RS, Brazil. RP Deiss, L (reprint author), Fed Univ Parana UFPR, Agr Sci Sect, Dept Crop Prod & Protect, Rua Funcionarios 1-540, BR-80035050 Curitiba, Parana, Brazil. EM leonardodeiss@gmail.com; anibalm@ufpr.br; jefersondieckow@ufpr.br; alan.franzluebbers@ars.usda.gov; gatiboni@udesc.br; sassaki@ufpn.br; paulocfc@ufrgs.br RI de Faccio Carvalho, Paulo Cesar/A-7381-2012; OI de Faccio Carvalho, Paulo Cesar/0000-0002-7020-3001; Deiss, Leonardo/0000-0003-2001-9238 FU Coordination for the Improvement of Higher Education Personnel (CAPES-Brazil); National Council for Scientific and Technological Development (CNPq-Brazil) FX The authors gratefully acknowledge the Coordination for the Improvement of Higher Education Personnel (CAPES-Brazil) and the National Council for Scientific and Technological Development (CNPq-Brazil) for funding this research. The authors would like to thank the anonymous reviewers for their thoughtful comments. NR 69 TC 0 Z9 0 U1 12 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD JUL 15 PY 2016 VL 274 BP 88 EP 96 DI 10.1016/j.geoderma.2016.03.028 PG 9 WC Soil Science SC Agriculture GA DM7MB UT WOS:000376543600010 ER PT J AU Salley, SW Sleezer, RO Bergstrom, RM Martin, PH Kelly, EF AF Salley, Shawn W. Sleezer, Richard O. Bergstrom, Robert M. Martin, Patrick H. Kelly, Eugene F. TI A long-term analysis of the historical dry boundary for the Great Plains of North America: Implications of climatic variability and climatic change on temporal and spatial patterns in soil moisture SO GEODERMA LA English DT Article DE Soil moisture; North American Great Plains; Newhall Simulation Model; Climatic variability; Calcic soils ID UNITED-STATES; REGIMES; CARBON; SYSTEM AB The boundary between the humid eastern and the arid western regions of the Great Plains of North America is of great economic interest and historic intrigue, yet its location is controversial. Areas to the east of this boundary have historically enjoyed the benefits of fertile soil coupled with more favorable rainfall and reliable surface water, permitting conventional agriculture to flourish over a remarkably large percentage of the eastern Great Plains. The expansion of population and agriculture during the nineteenth century across the western Great Plains tested the extent that non-irrigated, row crop agriculture could be successful in areas where year-to-year rainfall was unreliable. In this paper, we quantify the historic annual variability of soil moisture and hydrologic conditioning in the Great Plains resulting from climatic variability, show the regions that historically demonstrate unreliable precipitation, and identify the extent of arid regions of the central United States based on modeled annual soil moisture variability. We asked how arid climates have influenced soil formation patterns at small cartographic scales, and how soil properties buffer or enhance soil moisture regimes (at the udic-ustic boundary) to climate variability at larger cartographic scales. At small cartographic scales, a climate-only model worked nearly as well as a climate-and-soils model in mapping the region's soil moisture boundary; however, a climate-only model missed important local soil influences. Finally, we demonstrate that long-term climate and climate variability are reflected in the depth and concentration of the calcic soil properties. From a practical standpoint, our work highlights that soils with higher water holding capacity dampen periodic short-term rainfall deficits, while soils with lower water holding capacity can exhibit edaphic drought during otherwise normal climate years. Published by Elsevier B.V. C1 [Salley, Shawn W.] New Mexico State Univ, USDA ARS, Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. [Sleezer, Richard O.] Emporia State Univ, Coll Liberal Arts & Sci, Roosevelt Hall,1 Kellogg Circle, Emporia, KS 66801 USA. [Bergstrom, Robert M.] Univ West Georgia, Dept Geosci, Callaway Bldg,1601 Maple St, Carrollton, GA 30118 USA. [Martin, Patrick H.] Colorado State Univ, Dept Hort & Landscape Architecture, 1173 Campus Delivery, Ft Collins, CO 80523 USA. [Kelly, Eugene F.] Colorado State Univ, Dept Soil & Crop Sci, 1170 Campus Delivery, Ft Collins, CO 80523 USA. RP Salley, SW (reprint author), New Mexico State Univ, USDA ARS, Jornada Expt Range, MSC 3JER,Box 30003, Las Cruces, NM 88003 USA. EM shsalley@nmsu.edu; rsleezer@emporia.edu; rbergstr@westga.edu; patrick.martin@colostate.edu; eugene.kelly@colostate.edu OI Salley, Shawn/0000-0002-6092-0154 FU Shortgrass Steppe - Long Term Ecological Research site (SGS-LTER) - National Science Foundation [NSF DEB 0823405, NSF DEB 0217631] FX Authors thank two anonymous reviewers for their useful suggestions which greatly improved the manuscript. Financial support of this research was given by the Shortgrass Steppe - Long Term Ecological Research site (SGS-LTER) funded by the National Science Foundation (NSF DEB 0823405 and NSF DEB 0217631). Thanks to P. Finnell for help with Soil Data Access query and to C. Garton for editing multiple drafts of the manuscript. NR 51 TC 0 Z9 0 U1 4 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD JUL 15 PY 2016 VL 274 BP 104 EP 113 DI 10.1016/j.geoderma.2016.03.020 PG 10 WC Soil Science SC Agriculture GA DM7MB UT WOS:000376543600012 ER PT J AU Kalkhoff, SJ Hubbard, LE Tomer, MD James, DE AF Kalkhoff, S. J. Hubbard, L. E. Tomer, M. D. James, D. E. TI Effect of variable annual precipitation and nutrient input on nitrogen and phosphorus transport from two Midwestern agricultural watersheds SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Agriculture; Nutrient transport; Nutrient yield; Water quality; Land use ID GULF-OF-MEXICO; ANIMAL FEEDING OPERATIONS; MISSISSIPPI RIVER-BASIN; LIQUID SWINE MANURE; NITRATE-NITROGEN; MANAGEMENT-PRACTICES; SUBSURFACE DRAINS; QUALITY TRENDS; UNITED-STATES; IOWA RIVERS AB Precipitation patterns and nutrient inputs affect transport of nitrate (NO3-N) and phosphorus (TP) from Midwest watersheds. Nutrient concentrations and yields from two subsurface-drained watersheds, the Little Cobb River (LCR) in southern Minnesota and the South Fork Iowa River (SFIR) in northern Iowa, were evaluated during 1996-2007 to document relative differences in timings and amounts of nutrients transported. Both watersheds are located in the prairie pothole region, but the SFIR exhibits a longer growing season and more livestock production. The SFIR yielded significantly more NO3-N than the LCR watershed (31.2 versus 21.3 kg NO3-N ha(-1) y(-1)). The SFIR watershed also yielded more TP than the LCR watershed (1.13 versus 0.51 kg TP ha(-1) yr(-1)), despite greater TP concentrations in the LCR. About 65% of NO3-N and 50% of TP loads were transported during April-June, and <20% of the annual loads were transported later in the growing season from July-September. Monthly NO3-N and TP loads peaked in April from the LCR but peaked in June from the SFIR; this difference was attributed to greater snowmelt runoff in the LCR. The annual NO3-N yield increased with increasing annual runoff at a similar rate in both watersheds, but the LCR watershed yielded less annual NO3-N than the SFIR for a similar annual runoff. These two watersheds are within 150 km of one another and have similar dominant agricultural systems, but differences in climate and cropping inputs affected amounts and timing of nutrient transport. Published by Elsevier B.V. C1 [Kalkhoff, S. J.] US Geol Survey, 400 S Clinton St,Rm 269, Iowa City, IA 52240 USA. [Tomer, M. D.; James, D. E.] USDA, 2110 Univ Blvd, Ames, IA 50011 USA. [Hubbard, L. E.] US Geol Survey, 8505 Res Way, Middleton, WI 53562 USA. RP Kalkhoff, SJ (reprint author), US Geol Survey, 400 S Clinton St,Rm 269, Iowa City, IA 52240 USA. EM sjkalkho@usgs.gov OI Kalkhoff, Stephen/0000-0003-4110-1716 FU USGS National Water-Quality Assessment Program; USDA-NRCS Conservation Effects Assessment Project; South Fork Watershed Alliance FX The authors thank the staff of the USGS Iowa and Minnesota Water Science Centers for their assistance in sample collection. Advice on the use of the WRTDS model was provided by Bob Hirsch. Data collection and report preparation were funded by the USGS National Water-Quality Assessment Program. Research conducted by USDA-ARS in the South Fork Iowa River watershed was financially supported by the USDA-NRCS Conservation Effects Assessment Project, and locally supported by the South Fork Watershed Alliance. Initial versions of this report were substantially improved by suggestions from Victoria Christensen and several anonymous reviewers. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 46 TC 2 Z9 3 U1 11 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 15 PY 2016 VL 559 BP 53 EP 62 DI 10.1016/j.scitotenv.2016.03.127 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7WD UT WOS:000375136600007 PM 27054493 ER PT J AU Donovan, GH Jovan, SE Gatziolis, D Burstyn, I Michael, YL Amacher, MC Monleon, VJ AF Donovan, Geoffrey H. Jovan, Sarah E. Gatziolis, Demetrios Burstyn, Igor Michael, Yvonne L. Amacher, Michael C. Monleon, Vicente J. TI Using an epiphytic moss to identify previously unknown sources of atmospheric cadmium pollution SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Arsenic; Selenium; Air pollution; Stained-glass; Orthotrichum lyellii ID PSEUDOSCLEROPODIUM-PURUM; AIR-POLLUTION; HEAVY-METALS; DEPOSITION; EXPOSURE; CANCER; LEAD; CONTAMINATION; ACCUMULATION; BIOMONITORS AB Urban networks of air-quality monitors are often too widely spaced to identify sources of air pollutants, especially if they do not disperse far from emission sources. The objectives of this study were to test the use of moss bio-indicators to develop a fine-scale map of atmospherically-derived cadmium and to identify the sources of cadmium in a complex urban setting. We collected 346 samples of the moss Orthotrichum lyellii from deciduous trees in December, 2013 using a modified randomized grid-based sampling strategy across Portland, Oregon. We estimated a spatial linear model of moss cadmium levels and predicted cadmium on a 50 m grid across the city. Cadmium levels in moss were positively correlated with proximity to two stained-glass manufacturers, proximity to the Oregon-Washington border, and percent industrial land in a 500 m buffer, and negatively correlated with percent residential land in a 500 m buffer. The maps showed very high concentrations of cadmium around the two stained-glass manufacturers, neither of which were known to environmental regulators as cadmium emitters. In addition, in response to our findings, the Oregon Department of Environmental Quality placed an instrumental monitor 120 m from the larger stained-glass manufacturer in October, 2015. The monthly average atmospheric cadmium concentration was 29.4 ng/m(3), which is 49 times higher than Oregon's benchmark of 0.6 ng/m(3), and high enough to pose a health risk from even short-term exposure. Both stained-glass manufacturers voluntarily stopped using cadmium after the monitoring results were made public, and the monthly average cadmium levels precipitously dropped to 1.1 ng/m(3) for stained-glass manufacturer #1 and 0.67 ng/m(3) for stained-glass manufacturer #2. Published by Elsevier B.V. C1 [Donovan, Geoffrey H.; Jovan, Sarah E.; Gatziolis, Demetrios] US Forest Serv, USDA, PNW Res Stn, 620 SW Main,Suite 400, Portland, OR 97205 USA. [Burstyn, Igor; Michael, Yvonne L.] Drexel Univ, Dornsife Sch Publ Hlth, Nesbitt Hall,3215 Market St, Philadelphia, PA 19104 USA. [Monleon, Vicente J.] US Forest Serv, USDA, PNW Res Stn, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. [Amacher, Michael C.] US Forest Serv, USDA, Logan Forest Sci Lab, 860 North 1200 East, Logan, UT 84321 USA. RP Jovan, SE (reprint author), US Forest Serv, USDA, PNW Res Stn, 620 SW Main,Suite 400, Portland, OR 97205 USA. EM gdonovan@fs.fed.us; sjovan@fs.fed.us; dgatziolis@fs.fed.us; igor.burstyn@drexel.edu; ylm23@drexel.edu; mcamacher1@outlook.com; vjmonleon@fs.fed.us NR 42 TC 0 Z9 0 U1 17 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 15 PY 2016 VL 559 BP 84 EP 93 DI 10.1016/j.scitotenv.2016.03.182 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA DK7WD UT WOS:000375136600010 PM 27058127 ER PT J AU He, ZQ Chapital, DC Cheng, HN AF He, Zhongqi Chapital, Dorselyn C. Cheng, Huai N. TI Effects of pH and storage time on the adhesive and rheological properties of cottonseed meal-based products SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE adhesives; biopolymers and renewable polymers; proteins; rheology; viscosity; viscoelasticity ID SOY PROTEIN; WATER RESISTANCE; WOOD ADHESIVES; IMPROVE; MAPLE; OIL AB To increase the basic knowledge of cottonseed meal (CSM)-based adhesives and optimize the operational parameters for practical applications, in this study, we investigated the effects of pH and storage time on the adhesive performance, water resistance, and rheological properties of CSM, water-washed cottonseed meal (WCSM), and cottonseed protein isolate (CSPI). We found all products possessed the highest dry, wet, and soaked adhesive strengths with the adhesive slurries prepared at pH 6.0. The effects of pH were smaller on WCSM than on CSM and CSPI slurries. Storage time (up to 8 days) did not greatly impact the adhesive performance of WCSM slurries prepared at pH 6.0, 7.5, and 9.0, but slightly reduced the adhesive strength of CSPI slurries with the same pH. The viscosity of WCSM slurries increased with storage over 8 days, but did not for CSPI slurries. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43637. C1 [He, Zhongqi; Chapital, Dorselyn C.; Cheng, Huai N.] USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. RP He, ZQ (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Zhongqi.He@ars.usda.gov NR 35 TC 0 Z9 0 U1 9 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD JUL 15 PY 2016 VL 33 IS 27 AR 43637 DI 10.1002/app.43637 PG 7 WC Polymer Science SC Polymer Science GA DK1JI UT WOS:000374667900016 ER PT J AU Galdino, TVD Kumar, S Oliveira, LSS Alfenas, AC Neven, LG Al-Sadi, AM Picanco, MC AF da Silva Galdino, Tarcisio Visintin Kumar, Sunil Oliveira, Leonardo S. S. Alfenas, Acelino C. Neven, Lisa G. Al-Sadi, Abdullah M. Picanco, Marcelo C. TI Mapping Global Potential Risk of Mango Sudden Decline Disease Caused by Ceratocystis fimbriata SO PLOS ONE LA English DT Article ID PREDICTING SPECIES DISTRIBUTIONS; ECOLOGICAL NICHE MODELS; SULTANATE-OF-OMAN; MANGIFERA-INDICA; QUICK DECLINE; BARK BEETLE; PAKISTAN; PATHOGEN; AREAS; TREES AB The Mango Sudden Decline (MSD), also referred to as Mango Wilt, is an important disease of mango in Brazil, Oman and Pakistan. This fungus is mainly disseminated by the mango bark beetle, Hypocryphalus mangiferae (Stebbing), by infected plant material, and the infested soils where it is able to survive for long periods. The best way to avoid losses due to MSD is to prevent its establishment in mango production areas. Our objectives in this study were to: (1) predict the global potential distribution of MSD, (2) identify the mango growing areas that are under potential risk of MSD establishment, and (3) identify climatic factors associated with MSD distribution. Occurrence records were collected from Brazil, Oman and Pakistan where the disease is currently known to occur in mango. We used the correlative maximum entropy based model (MaxEnt) algorithm to assess the global potential distribution of MSD. The MaxEnt model predicted suitable areas in countries where the disease does not already occur in mango, but where mango is grown. Among these areas are the largest mango producers in the world including India, China, Thailand, Indonesia, and Mexico. The mean annual temperature, precipitation of coldest quarter, precipitation seasonality, and precipitation of driest month variables contributed most to the potential distribution of MSD disease. The mango bark beetle vector is known to occur beyond the locations where MSD currently exists and where the model predicted suitable areas, thus showing a high likelihood for disease establishment in areas predicted by our model. Our study is the first to map the potential risk of MSD establishment on a global scale. This information can be used in designing strategies to prevent introduction and establishment of MSD disease, and in preparation of efficient pest risk assessments and monitoring programs. C1 [da Silva Galdino, Tarcisio Visintin] Univ Fed Vicosa, Dept Plant Sci, Vicosa, MG, Brazil. [Kumar, Sunil] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Oliveira, Leonardo S. S.; Alfenas, Acelino C.] Univ Fed Vicosa, Dept Plant Pathol, Vicosa, MG, Brazil. [Neven, Lisa G.] USDA ARS, Yakima Agr Res Lab, Wapato, WA USA. [Al-Sadi, Abdullah M.] Sultan Qaboos Univ, Dept Crop Sci, Al Khoud, Oman. [Picanco, Marcelo C.] Univ Fed Vicosa, Dept Entomol, Vicosa, MG, Brazil. RP Galdino, TVD (reprint author), Univ Fed Vicosa, Dept Plant Sci, Vicosa, MG, Brazil. EM tarcsilva@gmail.com OI Galdino, Tarcisio/0000-0001-7317-0789; Al-Sadi, Abdullah/0000-0002-3419-8268 FU VALE; National Council of Scientific and Technological Development (CNPq); Minas Gerais State Foundation for Research Aid (FAPEMIG); CAPES Foundation (Brazilian Ministry of Education); VALE Oman; VALE SQU; U.S. Geological Survey; Washington Tree Fruit Research Commission (WTFRC) from the Foreign Agricultural Service of the U.S. Department of Agriculture (USDA) FX The authors are grateful for the financial support and fellowships provided by VALE, the National Council of Scientific and Technological Development (CNPq), the Minas Gerais State Foundation for Research Aid (FAPEMIG), CAPES Foundation (Brazilian Ministry of Education), VALE Oman and SQU. SK was partially supported by U.S. Geological Survey and a grant through the Washington Tree Fruit Research Commission (WTFRC) from the Foreign Agricultural Service of the U.S. Department of Agriculture (USDA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; We are grateful for the financial support and fellowships provided by VALE, the National Council of Scientific and Technological Development (CNPq), the Minas Gerais State Foundation for Research Aid (FAPEMIG), CAPES Foundation (Brazilian Ministry of Education), VALE Oman and SQU. SK was partially supported by U.S. Geological Survey and a grant through the Washington Tree Fruit Research Commission (WTFRC) from the Foreign Agricultural Service of the U.S. Department of Agriculture (USDA). We also thank the Universidade Federal de Vicosa, Brazil, and Natural Resource Ecology Laboratory at Colorado State University for providing the logistical support. We thank Senait Senay and Kylie Swisher for their careful review of our paper, and for the comments. Finally, we are grateful to three anonymous reviewers whose comments also improved the manuscript. NR 73 TC 0 Z9 0 U1 5 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 14 PY 2016 VL 11 IS 7 AR e0159450 DI 10.1371/journal.pone.0159450 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR0DZ UT WOS:000379579500129 PM 27415625 ER PT J AU Naegele, RP Mitchell, J Hausbeck, MK AF Naegele, Rachel P. Mitchell, Jenna Hausbeck, Mary K. TI Genetic Diversity, Population Structure, and Heritability of Fruit Traits in Capsicum annuum SO PLOS ONE LA English DT Article ID TOMATO ANALYZER SOFTWARE; MULTILOCUS GENOTYPE DATA; MARKER ANALYSIS; LATE BLIGHT; SHAPE; PEPPER; RESISTANCE; LOCI; QTL; INHERITANCE AB Cultivated pepper (Capsicum annuum) is a phenotypically diverse species grown throughout the world. Wild and landrace peppers are typically small-fruited and pungent, but contain many important traits such as insect and disease resistance. Cultivated peppers vary dramatically in size, shape, pungency, and color, and often lack resistance traits. Fruit characteristics (e.g. shape and pericarp thickness) are major determinants for cultivar selection, and their association with disease susceptibility can reduce breeding efficacy. This study evaluated a diverse collection of peppers for mature fruit phenotypic traits, correlation among fruit traits and Phytophthora fruit rot resistance, genetic diversity, population structure, and trait broad sense heritability. Significant differences within all fruit phenotype categories were detected among pepper lines. Fruit from Europe had the thickest pericarp, and fruit from Ecuador had the thinnest. For fruit shape index, fruit from Africa had the highest index, while fruit from Europe had the lowest. Five genetic clusters were detected in the pepper population and were significantly associated with fruit thickness, end shape, and fruit shape index. The genetic differentiation between clusters ranged from little to very great differentiation when grouped by the predefined categories. Broad sense heritability for fruit traits ranged from 0.56 (shoulder height) to 0.98 (pericarp thickness). When correlations among fruit phenotypes and fruit disease were evaluated, fruit shape index was negatively correlated with pericarp thickness, and positively correlated with fruit perimeter. Pepper fruit pericarp, perimeter, and width had a slight positive correlation with Phytophthora fruit rot, whereas fruit shape index had a slight negative correlation. C1 [Naegele, Rachel P.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA USA. [Mitchell, Jenna; Hausbeck, Mary K.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Hausbeck, MK (reprint author), Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. EM hausbec1@msu.edu NR 45 TC 0 Z9 0 U1 11 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUL 14 PY 2016 VL 11 IS 7 AR e0156969 DI 10.1371/journal.pone.0156969 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR0DZ UT WOS:000379579500012 PM 27415818 ER PT J AU McPhillie, M Zhou, Y El Bissati, K Dubey, J Lorenzi, H Capper, M Lukens, AK Hickman, M Muench, S Verma, SK Weber, CR Wheeler, K Gordon, J Sanders, J Moulton, H Wang, K Kim, TK He, YQ Santos, T Woods, S Lee, P Donkin, D Kim, E Fraczek, L Lykins, J Esaa, F Alibana-Clouser, F Dovgin, S Weiss, L Brasseur, G Wirth, D Kent, M Hood, L Meunieur, B Roberts, CW Hasnain, SS Antonyuk, SV Fishwick, C McLeod, R AF McPhillie, Martin Zhou, Ying El Bissati, Kamal Dubey, Jitender Lorenzi, Hernan Capper, Michael Lukens, Amanda K. Hickman, Mark Muench, Stephen Verma, Shiv Kumar Weber, Christopher R. Wheeler, Kelsey Gordon, James Sanders, Justin Moulton, Hong Wang, Kai Kim, Taek-Kyun He, Yuqing Santos, Tatiana Woods, Stuart Lee, Patty Donkin, David Kim, Eric Fraczek, Laura Lykins, Joseph Esaa, Farida Alibana-Clouser, Fatima Dovgin, Sarah Weiss, Louis Brasseur, Gael Wirth, Dyann Kent, Michael Hood, Leroy Meunieur, Brigitte Roberts, Craig W. Hasnain, S. Samar Antonyuk, Svetlana V. Fishwick, Colin McLeod, Rima TI New paradigms for understanding and step changes in treating active and chronic, persistent apicomplexan infections SO SCIENTIFIC REPORTS LA English DT Article ID ACQUIRED-IMMUNODEFICIENCY-SYNDROME; CYTOCHROME BC(1) COMPLEX; TOXOPLASMA-GONDII; PLASMODIUM-FALCIPARUM; IN-VITRO; CONGENITAL TOXOPLASMOSIS; ELECTRON-TRANSPORT; NERVOUS-SYSTEM; REDUCTION SITE; ATOVAQUONE AB Toxoplasma gondii, the most common parasitic infection of human brain and eye, persists across lifetimes, can progressively damage sight, and is currently incurable. New, curative medicines are needed urgently. Herein, we develop novel models to facilitate drug development: EGS strain T. gondii forms cysts in vitro that induce oocysts in cats, the gold standard criterion for cysts. These cysts highly express cytochrome b. Using these models, we envisioned, and then created, novel 4-(1H)-quinolone scaffolds that target the cytochrome bc(1) complex Q(i) site, of which, a substituted 5,6,7,8-tetrahydroquinolin-4-one inhibits active infection (IC50, 30 nM) and cysts (IC50, 4 mu M) in vitro, and in vivo (25 mg/kg), and drug resistant Plasmodium falciparum (IC50, < 30 nM), with clinically relevant synergy. Mutant yeast and co-crystallographic studies demonstrate binding to the bc(1) complex Q(i) site. Our results have direct impact on improving outcomes for those with toxoplasmosis, malaria, and similar to 2 billion persons chronically infected with encysted bradyzoites. C1 [McPhillie, Martin; Muench, Stephen; Gordon, James; Fishwick, Colin] Univ Leeds, Leeds, W Yorkshire, England. [Zhou, Ying; El Bissati, Kamal; Weber, Christopher R.; Wheeler, Kelsey; Fraczek, Laura; Lykins, Joseph; Esaa, Farida; Alibana-Clouser, Fatima; Dovgin, Sarah; McLeod, Rima] Univ Chicago, Chicago, IL 60637 USA. [Dubey, Jitender; Verma, Shiv Kumar] USDA, Beltsville, MD 20705 USA. [Lorenzi, Hernan] J Craig Venter Inst, Rockville, MD USA. [Capper, Michael; Hasnain, S. Samar; Antonyuk, Svetlana V.] Univ Liverpool, Liverpool, Merseyside, England. [Lukens, Amanda K.; Wirth, Dyann] Harvard Univ, Sch Publ Hlth, 665 Huntington Ave, Boston, MA 02115 USA. [Lukens, Amanda K.; Wirth, Dyann] Broad Inst, Boston, MA USA. [Hickman, Mark; Lee, Patty; Donkin, David; Kim, Eric] Walter Reed Army Inst Res, Silver Spring, MD USA. [Sanders, Justin; Moulton, Hong; Kent, Michael] Oregon State Univ, Corvallis, OR 97331 USA. [Wang, Kai; Kim, Taek-Kyun; He, Yuqing; Hood, Leroy] Inst Syst Biol, Seattle, WA USA. [Santos, Tatiana; Weiss, Louis] Albert Einstein Coll Med, Bronx, NY 10467 USA. [Woods, Stuart; Roberts, Craig W.] Strathclyde Univ, Glasgow, Lanark, Scotland. [Brasseur, Gael] CNRS, Marseilles, France. [Meunieur, Brigitte] Inst Integrat Biol Cell 12BC, Gif Sur Yvette, France. RP Fishwick, C (reprint author), Univ Leeds, Leeds, W Yorkshire, England.; McLeod, R (reprint author), Univ Chicago, Chicago, IL 60637 USA.; Antonyuk, SV (reprint author), Univ Liverpool, Liverpool, Merseyside, England. EM antonyuk@liverpool.ac.uk; c.w.g.fishwick@leeds.ac.uk; rmcleod@uchicago.edu OI Roberts, Craig/0000-0002-0653-835X FU NIH NIAID DMID [AI U01AI082180]; Mann Cornwell Family; Engel family and "Taking out Toxo"; Rooney family; Morel family; Wellcome Trust; BBSRC; National Institute of Allergy and Infectious Diseases of the National Institutes of Health [HHSN272200900007C, U19AI110819]; DTRA; DOD [HDTRA1-13-C-0055, W911NF-09-D0001, W911SR-07-C0101] FX This work was supported by Grant number AI U01AI082180 from NIH NIAID DMID and by the Mann Cornwell Family, the Engel family and "Taking out Toxo", the Rooney and the Morel families. University of Liverpool team was supported via Wellcome Trust and BBSRC and would like to acknowledge on-going collaboration with Professor Paul O'Neil (Chemistry, U of Liverpool) and Prof Giancarlo Biangini (LSTM) on a related project on Plasmodium falciparum. We acknowledge the support of crystallographic facilities at DIAMOND (UK). Research reported in this publication also was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Contract Number HHSN272200900007C and Award Number U19AI110819. The team at the Institute for Systems Biology is partially supported by research contracts from DTRA and DOD (HDTRA1-13-C-0055, W911NF-09-D0001 and W911SR-07-C0101). We acknowledge Openeye Inc for the generous provision of an Academic software license. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We thank Ian Begeman for his assistance in the preparation of this manuscript. We thank Eleonor Lago MD PhD for providing the cost analysis data for Brazil. NR 65 TC 0 Z9 0 U1 4 U2 4 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 14 PY 2016 VL 6 AR 29179 DI 10.1038/srep29179 PG 23 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DQ9YS UT WOS:000379565800001 PM 27412848 ER PT J AU Mogren, CL Lundgren, JG AF Mogren, Christina L. Lundgren, Jonathan G. TI Neonicotinoid-contaminated pollinator strips adjacent to cropland reduce honey bee nutritional status SO SCIENTIFIC REPORTS LA English DT Article ID SEED TREATMENTS; INSECTICIDE RESIDUES; RAPID-DETERMINATION; OILSEED RAPE; EXPOSURE; THIAMETHOXAM; CROPS; CORN; CLOTHIANIDIN; MOSQUITOS AB Worldwide pollinator declines are attributed to a number of factors, including pesticide exposures. Neonicotinoid insecticides specifically have been detected in surface waters, non-target vegetation, and bee products, but the risks posed by environmental exposures are still not well understood. Pollinator strips were tested for clothianidin contamination in plant tissues, and the risks to honey bees assessed. An enzyme-linked immunosorbent assay (ELISA) quantified clothianidin in leaf, nectar, honey, and bee bread at organic and seed-treated farms. Total glycogen, lipids, and protein from honey bee workers were quantified. The proportion of plants testing positive for clothianidin were the same between treatments. Leaf tissue and honey had similar concentrations of clothianidin between organic and seed-treated farms. Honey (mean +/- SE: 6.61 +/- 0.88 ppb clothianidin per hive) had seven times greater concentrations than nectar collected by bees (0.94 +/- 0.09 ppb). Bee bread collected from organic sites (25.8 +/- 3.0 ppb) had significantly less clothianidin than those at seed treated locations (41.6 +/- 2.9 ppb). Increasing concentrations of clothianidin in bee bread were correlated with decreased glycogen, lipid, and protein in workers. This study shows that small, isolated areas set aside for conservation do not provide spatial or temporal relief from neonicotinoid exposures in agricultural regions where their use is largely prophylactic. C1 [Mogren, Christina L.; Lundgren, Jonathan G.] USDA ARS, 2923 Medary Ave, Brookings, SD 57006 USA. [Mogren, Christina L.] Louisiana State Univ, AgCtr, Life Sci 404, Baton Rouge, LA 70803 USA. [Lundgren, Jonathan G.] Ecdysis Fdn Blue Dash Farm Initiat, 46958 188th St, Blue Dash, SD 57234 USA. RP Mogren, CL (reprint author), USDA ARS, 2923 Medary Ave, Brookings, SD 57006 USA.; Mogren, CL (reprint author), Louisiana State Univ, AgCtr, Life Sci 404, Baton Rouge, LA 70803 USA. EM cmogren@gmail.com FU National Honey Board; USDA-ELI grant [2016-67012-24826] FX We would like to thank Claire Bestul, Michael Bredeson, Janet Fergen, Dakotah Grosz, Kae Januschka, Nathan Koens, Marti LaValle, Abigail Martens, Jacob Pecenka, Greta Schen, Casey Snyder, and Cally Strobel for their assistance with data collection. We would also like to thank Adee Honey Farms (Bruce, SD) and the farmers for providing access to bee hives and land for the duration of this study. This research was funded by two years of support from the National Honey Board and USDA-ELI grant 2016-67012-24826. Mention of any proprietary products does not constitute endorsement by the USDA-ARS. NR 59 TC 4 Z9 4 U1 34 U2 63 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD JUL 14 PY 2016 VL 6 AR 29608 DI 10.1038/srep29608 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA DR0CR UT WOS:000379576100001 PM 27412495 ER PT J AU Nigro, D Fortunato, S Giove, SL Paradiso, A Gu, YQ Blanco, A de Pinto, MC Gadaleta, A AF Nigro, Domenica Fortunato, Stefania Giove, Stefania L. Paradiso, Annalisa Gu, Yong Q. Blanco, Antonio de Pinto, Maria C. Gadaleta, Agata TI Glutamine synthetase in Durum Wheat: Genotypic Variation and Relationship with Grain Protein Content SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE wheat; grain protein content; GS (Glutamine synthetase); qRT-PCR; enzyme activity; western blot ID NITROGEN-USE EFFICIENCY; TRITICUM-AESTIVUM L.; GENES; RICE; IDENTIFICATION; NORMALIZATION; IMPROVEMENT; EXPRESSION; FERTILIZER; CULTIVARS AB Grain protein content (GPO), is one of the most important trait in wheat and its characterized by a very complex genetic control. The identification of wheat varieties with high GPC (HGPC), as well as the characterization of central enzymes involved in these processes, are important for more sustainable agricultural practices. In this study, we focused on Glutamine synthetase (GS) as a candidate to study GPC in wheat. We analyzed GS expression and its enzymatic activity in different tissues and phenological stages in 10 durum wheat genotypes with different GPC. Although each genotype performed quite differently from the others, both because their genetic variability and their adaptability to specific environmental conditions, the highest GS activity and expression were found in genotypes with HGPC and vice versa the lowest ones in genotypes with low GPC (LGPC). Moreover, in genotypes contrasting in GPC bred at different nitrogen regimes (0, 60, 140 N Unit/ha) GS behaved differently in diverse organs. Nitrogen supplement increased GS expression and activity in roots of all genotypes, highlighting the key role of this enzyme in nitrogen assimilation and ammonium detoxification in roots. Otherwise, nitrogen treatments decreased GS expression and activity in the leaves of HGPC genotypes and did not affect GS in the leaves of LGPC genotypes. Finally, no changes in GS and soluble protein content occurred at the filling stage in the caryopses of all analyzed genotypes. C1 [Nigro, Domenica; Fortunato, Stefania; Giove, Stefania L.; Blanco, Antonio] Univ Bari Aldo Moro, Dept Soil Plant & Food Sci, Bari, Italy. [Fortunato, Stefania; Gadaleta, Agata] Univ Bari Aldo Moro, Res Unity Genet & Plant Biotechnol, Dept Agr & Environm Sci, Bari, Italy. [Paradiso, Annalisa; de Pinto, Maria C.] Univ Bari Aldo Moro, Dept Biol, Bari, Italy. [Gu, Yong Q.] USDA ARS, Western Reg Res Ctr, Crop Improvement & Genet Res, 800 Buchanan St, Albany, CA 94710 USA. RP Gadaleta, A (reprint author), Univ Bari Aldo Moro, Res Unity Genet & Plant Biotechnol, Dept Agr & Environm Sci, Bari, Italy. EM agata.gadaleta@uniba.it FU Ministero dell' University e della Ricerca, Italy, project: PON-ISCOCEM; Ministero dell' University e della Ricerca, Italy, project: PRIN; Regione Puglia, project: Future in Research FX This research was supported by a grant from Ministero dell' University e della Ricerca, Italy, projects: PON-ISCOCEM and PRIN 2010-11; and from Regione Puglia, project: Future in Research. NR 41 TC 1 Z9 1 U1 14 U2 22 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUL 13 PY 2016 VL 7 AR 971 DI 10.3389/fpls.2016.00971 PG 11 WC Plant Sciences SC Plant Sciences GA DQ9ZD UT WOS:000379566900001 PM 27468287 ER EF