FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Waters, GS Caplan, D AF Waters, GS Caplan, D TI The measurement of verbal working memory capacity and its relation to reading comprehension SO QUARTERLY JOURNAL OF EXPERIMENTAL PSYCHOLOGY SECTION A-HUMAN EXPERIMENTAL PSYCHOLOGY LA English DT Article; Proceedings Paper CT International Conference on Working Memory CY 1994 CL CAMBRIDGE, ENGLAND ID INDIVIDUAL-DIFFERENCES; FRONTAL-CORTEX; TEXT AB Ninety-four subjects were tested on the Daneman and Carpenter (1980) reading span task, four versions of a related sentence span task in which reaction times and accuracy on sentence processing were measured along with sentence-final word recall, two number generation tasks designed to test working memory, digit span, and two shape-generation tasks designed to measure visual-spatial working memory. Forty-four subjects were retested on a subset of these measures at a 3-month interval. All subjects were tested on standard vocabulary and reading tests. Correlational analyses showed better internal consistency and test-retest reliability of the sentence span tasks than of the Daneman-Carpenter reading span task. Factor analysis showed no factor that could be related to a central verbal working memory; rotated factors suggested groupings of tests into factors that correspond to digit-related tasks, spatial tasks, sentence processing in sentence span tasks, and recall in sentence span tasks. Correlational analyses and regression analyses showed that the sentence processing component of the sentence span tasks was the best predictor of performance on the reading test, with a small independent contribution of the recall component. The results suggest that sentence span tasks are unreliable unless measurements are made of both their sentence processing and recall components, and that the predictive value of these tasks for reading comprehension abilities lies in the overlap of operations rather than in limitations in verbal working memory that apply to both. C1 MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114. RP Waters, GS (reprint author), MCGILL UNIV,SCH COMMUN SCI & DISORDERS,1266 PINE AVE W,MONTREAL,PQ H3G 1A8,CANADA. FU NIA NIH HHS [AGO9661] NR 26 TC 184 Z9 185 U1 4 U2 25 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE, EAST SUSSEX, ENGLAND BN3 2FA SN 0272-4987 J9 Q J EXP PSYCHOL-A JI Q. J. Exp. Psychol. Sect A-Hum. Exp. Psychol. PD FEB PY 1996 VL 49 IS 1 BP 51 EP 79 DI 10.1080/027249896392801 PG 29 WC Psychology; Psychology, Experimental SC Psychology GA UF604 UT WOS:A1996UF60400004 PM 8920099 ER PT J AU Reimer, P Weissleder, R AF Reimer, P Weissleder, R TI Development and experimental application of receptor-specific MR contrast media SO RADIOLOGE LA German DT Article DE receptors; specific attachment; function ID SUPERPARAMAGNETIC IRON-OXIDE; HEPATIC BINDING-PROTEIN; RAT-LIVER; ASIALOGLYCOPROTEIN RECEPTORS; DESIALYLATED GLYCOPROTEINS; PRECLINICAL EVALUATION; PANCREATIC ACINI; ANIMAL-MODELS; CHOLECYSTOKININ; CELLS AB The authors describe the feasibility of developing receptor-specific MR contrast agents for the improved detection of pathology and assessment of organ function. Receptor specificity of MR contrast agents can be achieved by binding of receptor-specific carriers to ligands. This concept leads towards a decrease in dose and thus in toxicity. Specific attachment to parenchymal cells improves tumor-organ contrast and therefore tumor detection. Specific uptake mechanisms also enable the assessment of organ function. Future design concepts of novel MR contrast agents may consider the desired uptake in specific cells or organs (ovaries, adrenal glands, lymph nodes etc.) with subsequent synthesis of appropriate carriers. C1 MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. RP Reimer, P (reprint author), UNIV MUNSTER,INST KLIN RADIOL,ALBERT SCHWEITZER STR 33,D-48129 MUNSTER,GERMANY. NR 77 TC 49 Z9 54 U1 0 U2 6 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0033-832X J9 RADIOLOGE JI Radiologe PD FEB PY 1996 VL 36 IS 2 BP 153 EP 163 DI 10.1007/s001170050053 PG 11 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TY611 UT WOS:A1996TY61100008 PM 8867433 ER PT J AU Harika, L Weissleder, R Poss, K Papisov, MI AF Harika, L Weissleder, R Poss, K Papisov, MI TI Macromolecular intravenous contrast agent for MR lymphography: Characterization and efficacy studies SO RADIOLOGY LA English DT Article DE computed tomography (CT), contrast media; gadolinium; indium, radioactive; lymphatic system, MR; lymphatic system, neoplasms; lymphatic system, radionuclide studies; magnetic resonance (MR), contrast enhancement ID SUPERPARAMAGNETIC IRON-OXIDE; LYMPH-NODES; COMPUTED-TOMOGRAPHY; HODGKIN DISEASE; LYMPHOSCINTIGRAPHY; METASTASES; ANATOMY; CANCER AB PURPOSE: To determine the pharmacokinetic and magnetic resonance (MR) imaging properties of diethylenetriaminepentaacetic acid (DTPA) conjugated with a polyglucose-associated macrocomplex (PGM), which accumulates in lymph nodes. MATERIALS AND METHODS: In 124 normal and 20 tumor-bearing rats, Gd-DTPA PGM was administered intravenously in doses of 2, 10, 20 mu mol gadolinium per kilogram of tissue. RESULTS: Mean blood half-life was 2 hours. Maximum accumulation in peripheral (33.0% injected dose [ID]/ g +/- 16.2 [standard deviation]) and central lymph nodes (63.2% ID/g +/- 16.5) was observed within 24 hours after administration. The optimum dose range was 10-20 mu mol Gd/kg in rats. At 24 hours after administration of 20 mu mol Gd/kg, the signal-to-noise ratio increased from 30.9 +/- 0.4 to 83.2 +/- 5.2 in normal lymph nodes (P < .001 ). Differentiation between normal and metastatic lymph nodes was improved. CONCLUSION When labeled with Gd-DTPA, the PGM-based graft copolymer significantly increases signal intensity at MR imaging of normal but not metastatic lymph nodes without causing distortion artifacts. C1 MASSACHUSETTS GEN HOSP,NMR,DEPT RADIOL,MAGNET RESONANCE PHARMACEUT PROGRAM,BOSTON,MA 02129. HARVARD UNIV,SCH MED,BOSTON,MA 02129. FU NCI NIH HHS [R01 CA 59649-01] NR 25 TC 41 Z9 43 U1 0 U2 3 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD FEB PY 1996 VL 198 IS 2 BP 365 EP 370 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TR363 UT WOS:A1996TR36300014 PM 8596833 ER PT J AU Kaufman, JA Geller, SC Waltman, AC AF Kaufman, JA Geller, SC Waltman, AC TI Renal insufficiency: Gadopentetate dimeglumine as a radiographic contrast agent during peripheral vascular interventional procedures SO RADIOLOGY LA English DT Article DE digital subtraction angiography; gadolinium; kidney, effects of drugs on; kidney, failure ID DYSFUNCTION; ANGIOGRAPHY; MEDIA; DTPA AB Gadopentetate dimeglumine diluted 1:1 with 0.9% normal saline was used as the radiographic contrast agent in two patients with azotemia who underwent peripheral vascular interventional procedures. The patients had no evidence of contrast material-induced renal failure after the procedures. The radiographic attenuation of the diluted gadopentetate dimeglumine was equivalent to diatrizoate meglumine diluted to 40 mg iodine per milliliter. Gadopentetate dimeglumine is an alternative radiographic contrast material for azotemic patients. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. RP Kaufman, JA (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIOL,32 FRUIT ST,BOSTON,MA 02114, USA. NR 16 TC 80 Z9 83 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD FEB PY 1996 VL 198 IS 2 BP 579 EP 581 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TR363 UT WOS:A1996TR36300050 PM 8596869 ER PT J AU Mukherji, SK Curtin, HD Hudgins, PA Lufkin, RB Rao, VM Reede, DL Som, PM Swartz, JD AF Mukherji, SK Curtin, HD Hudgins, PA Lufkin, RB Rao, VM Reede, DL Som, PM Swartz, JD TI Head and neck radiology SO RADIOLOGY LA English DT Article; Proceedings Paper CT 81st Scientific Assembly and Annual Meeting of the Radiological-Society-of-North-America (RSNA 95) CY NOV 26-DEC 01, 1995 CL CHICAGO, IL SP Radiol Soc N Amer DE head, CT; head, MR; head and neck neoplasms, therapeutic radiology; Radiological Society of North America, 81st scientific assembly and annual; meeting C1 HARVARD UNIV,MASSACHUSETTS EYE & EAR INFIRM,SCH MED,BOSTON,MA. EMORY UNIV,SCH MED,ATLANTA,GA. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. THOMAS JEFFERSON UNIV HOSP,PHILADELPHIA,PA 19107. LONG ISL COLL HOSP,BROOKLYN,NY 11201. MT SINAI HOSP,NEW YORK,NY 10029. GERMANTOWN HOSP & MED CTR,PHILADELPHIA,PA. RP Mukherji, SK (reprint author), UNIV N CAROLINA,SCH MED,CHAPEL HILL,NC 27599, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU RADIOLOGICAL SOC NORTH AMER PI EASTON PA 20TH AND NORTHAMPTON STS, EASTON, PA 18042 SN 0033-8419 J9 RADIOLOGY JI Radiology PD FEB PY 1996 VL 198 IS 2 BP 616 EP 618 PG 3 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TR363 UT WOS:A1996TR36300065 PM 8596880 ER PT J AU Badr, MS Kawak, A AF Badr, MS Kawak, A TI Post-hyperventilation hypopnea in humans during NREM sleep SO RESPIRATION PHYSIOLOGY LA English DT Article DE control of breathing, central apnea, sleep; hypocapnia; mammals, human; sleep, NREM, central apnea; ventilation, mechanical ID MECHANICAL VENTILATION; MUSCLE-ACTIVITY; AFTERDISCHARGE; INHIBITION AB We wished to determine if mild hypocapnia above the ''apneic threshold'' would result in apnea or hypopnea during NREM sleep. Hypocapnia was induced by nasal mechanical hyperventilation for 1 min either under normoxia (51 trials, n = 7) or hyperoxia (43 trials, n = 5). Cessation of mechanical ventilation resulted in hypopnea due to reduced VT without a change in f. Central apnea occurred mostly under hyperoxic conditions (9/43 versus 2/51 trials under normoxic conditions), and only when complete inhibition of ventilatory motor output occurred during mechanical ventilation. Significant correlation between the magnitude of hypocapnia and nadir Vover dotE was noted under both normoxic and hyperoxic conditions. However, nadir Vover dotE was variable when hypocapnia was modest (-2 mmHg); further hypocapnia (-4 mmHg) was associated with consistent reduction in nadir Vover dotE below 30% of control under normoxic conditions, and central apnea under hyperoxic conditions. We conclude that: (1) Brief hyperventilation during NREM sleep is followed by hypocapnic hypopnea due to reduced VT and not breathing frequency; (2) Hypocapnia due to brief mild hyperventilation does not cause central apnea unless peripheral chemoreceptors are also inhibited; (3) Sustained hyperventilation or more severe hypocapnia may be required for the development of hypocapnic central apnea during NREM sleep. C1 UNIV WISCONSIN,SCH MED,DEPT MED,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT PREVENT MED,MADISON,WI 53705. RP Badr, MS (reprint author), UNIV WISCONSIN,SCH MED,WILLIAM S MIDDLETON MEM VET HOSP,MED SERV,MADISON,WI 53705, USA. FU NHLBI NIH HHS [HL-02588] NR 22 TC 20 Z9 20 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0034-5687 J9 RESP PHYSIOL JI Respir. Physiol. PD FEB PY 1996 VL 103 IS 2 BP 137 EP 145 PG 9 WC Physiology; Respiratory System SC Physiology; Respiratory System GA TY930 UT WOS:A1996TY93000004 PM 8833545 ER PT J AU Anderson, AC Scaringe, SA Earp, BE Frederick, CA AF Anderson, AC Scaringe, SA Earp, BE Frederick, CA TI HPLC purification of RNA for crystallography and NMR SO RNA-A PUBLICATION OF THE RNA SOCIETY LA English DT Article DE chromatography; oligonucleotide synthesis; pseudoknot; RNA crystallography ID MOSAIC-VIRUS RNA; CHEMICAL SYNTHESIS AB Homogeneous preparations of milligram quantities of RNA are a prerequisite for their characterization by biophysical methods such as crystallography or NMR spectroscopy. Methods for obtaining milligram quantities of pure synthetic RNA are described in this paper. These methods employ anion exchange HPLC for purifying full-length sequence from failure sequences and incompletely deprotected material. RNA molecules with little or extensive amounts of secondary structure could be purified. In cases where the RNA molecule was tightly folded, the cation in the eluent buffer influenced both the distinction of the peaks during chromatography and the final folded conformation. Finally, two RNA sequences were chemically synthesized, deprotected, purified, and crystallized using this methodology. C1 DANA FARBER CANC INST,BOSTON,MA 02115. HARVARD UNIV,COMMM BIOPHYS,BOSTON,MA 02115. UNIV COLORADO,DEPT CHEM & BIOCHEM,BOULDER,CO 80309. NR 10 TC 32 Z9 34 U1 1 U2 2 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 SN 1355-8382 J9 RNA JI RNA-Publ. RNA Soc. PD FEB PY 1996 VL 2 IS 2 BP 110 EP 117 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA UD294 UT WOS:A1996UD29400002 PM 8601278 ER PT J AU Ames, D Wirshing, WC Marder, SR Hwang, SS German, CA Strough, AB AF Ames, D Wirshing, WC Marder, SR Hwang, SS German, CA Strough, AB TI Subjective response to risperidone and haloperidol: Preliminary results SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD FEB PY 1996 VL 18 IS 2-3 BP VB1 EP VB1 PG 1 WC Psychiatry SC Psychiatry GA UE365 UT WOS:A1996UE36500100 ER PT J AU Ames, D Wirshing, WC Marder, SR Hwang, SS German, CA Mintz, J Goldstein, D AF Ames, D Wirshing, WC Marder, SR Hwang, SS German, CA Mintz, J Goldstein, D TI Risperidone vs haloperidol: Relative liabilities for OCD and depression SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RI Mintz, Jim/N-7385-2014 OI Mintz, Jim/0000-0002-8299-5851 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD FEB PY 1996 VL 18 IS 2-3 BP VB2 EP VB2 PG 1 WC Psychiatry SC Psychiatry GA UE365 UT WOS:A1996UE36500101 ER PT J AU Wirshing, WC Ames, D Marder, SR Marshall, BD Green, MF McGurk, S AF Wirshing, WC Ames, D Marder, SR Marshall, BD Green, MF McGurk, S TI Risperidone vs haloperidol in treatment resistant schizophrenia: Preliminary results SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD FEB PY 1996 VL 18 IS 2-3 BP VB5 EP VB5 PG 1 WC Psychiatry SC Psychiatry GA UE365 UT WOS:A1996UE36500104 ER PT J AU Chen, SY Marasco, WA AF Chen, SY Marasco, WA TI Novel genetic immunotoxins and intracellular antibodies for cancer therapy SO SEMINARS IN ONCOLOGY LA English DT Article ID GROWTH-FACTOR RECEPTOR; IMMUNODEFICIENCY-VIRUS TYPE-1; MONOCLONAL-ANTIBODY; PSEUDOMONAS EXOTOXIN; OVARIAN-CANCER; NEU ONCOGENE; HUMAN-BREAST; EXPRESSION; CELLS; PROTEIN C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV HUMAN RETROVIROL,BOSTON,MA 02115. RP Chen, SY (reprint author), WAKE FOREST UNIV,BOWMAN GRAY SCH MED,CTR COMPREHENS CANC,DEPT CANC BIOL,300 S HAWTHORNE RD,WINSTON SALEM,NC 27157, USA. NR 55 TC 15 Z9 18 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD FEB PY 1996 VL 23 IS 1 BP 148 EP 153 PG 6 WC Oncology SC Oncology GA TY194 UT WOS:A1996TY19400017 PM 8607024 ER PT J AU Palmer, WE AF Palmer, WE TI MR arthrography: Is it worthwhile? SO TOPICS IN MAGNETIC RESONANCE IMAGING LA English DT Article DE arthrography; gadolinium; shoulder; hip; knee; ankle; elbow; wrist ID 3-COMPARTMENT WRIST ARTHROGRAPHY; ROTATOR CUFF; ARTICULAR-CARTILAGE; OSTEOCHONDRITIS-DISSECANS; ARTHROSCOPIC REPAIR; CT ARTHROGRAPHY; GLENOID LABRUM; MENISCAL TEARS; SHOULDER; KNEE AB Magnetic resonance (MR) arthrography is possible in any joint in which standard arthrography is performed. The diagnostic potential of this technique is optimized in the assessment of complex anatomical structures and intra-articular abnormalities that are difficult to visualize on conventional MR images. This paper presents the most common applications of MR arthrography in the shoulder, hip, knee, ankle, elbow and wrist, and explores the clinical indications in which MR arthrography adds the greatest benefit compared to standard arthrography and conventional MR imaging. Arthrographic MR images are most valuable in the evaluation of glenoid and acetabular labra, tendons of the rotator cuff, post-operative menisci, osteochondral fractures and loose bodies. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. RP Palmer, WE (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIOL,WACC SUITE 515,15 PARKMAN ST,BOSTON,MA 02114, USA. NR 49 TC 28 Z9 28 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0899-3459 J9 TOP MAGN RESON IMAG JI Top. Magn. Reson. Imaging PD FEB PY 1996 VL 8 IS 1 BP 24 EP 43 PG 20 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TU268 UT WOS:A1996TU26800004 PM 8820092 ER PT J AU Ohto, H Anderson, KC AF Ohto, H Anderson, KC TI Posttransfusion graft-versus-host disease in Japanese newborns SO TRANSFUSION LA English DT Article ID LIVER-TRANSPLANT RECIPIENT; PREMATURE-INFANT; BLOOD-TRANSFUSIONS; THYMIC INVOLUTION; PREVENTION; DYSPLASIA; TOLERANCE; CELL AB Background: Posttransfusion graft-versus-host disease (PT-GVHD) is underdiagnosed and underreported. Risk factors predisposing to PT-GVHD in newborn recipients, the clinical manifestations of the disease in newborns, and its mechanism are not well characterized. Study Design and Methods: A literature review identified 27 cases of PT-GVHD in newborns in Japan. Detailed information on volume of blood transfused, donor(s), and clinical course was analyzed. Infants with known immunodeficiency were excluded. Results: Of 27 newborns, 20 were premature and 7 were full-term. Thirteen premature neonates were transfused frequently because of anemia; in 10 cases, exchange transfusion was performed. Fresh blood (less than or equal to 72 hours after donation) and blood from family member(s) were transfused to 25 and 22 neonates, respectively. The clinical manifestations of PT-GVHD developed later after transfusion than is reported in adults; the median intervals were: fever, 28 days; erythematous rash, 30 days; leukopenia, 43 days; and death, 51 days. Thymic damage was a striking feature among newborns. Two thirds of the cases of PT-GVHD were associated with overwhelming infections, and all patients died in spite of various treatments. Conclusion: These data suggest that cellular blood components, especially those from family members, should ge irradiated before transfusion to premature and fullterm neonates. Extrathymic and/or thymic semitolerance for allogeneic-donor cytotoxic T-lymphocytes may explain the longer period of latency before the onset of clinical manifestations and the longer course of disease before death in newborns than are noted in adults, as well as the fewer than expected reported cases of PT-GVHD in neonates. C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA. DANA FARBER CANC INST,DIV MED ONCOL,BLOOOD COMPONENT LAB,BOSTON,MA 02115. DANA FARBER CANC INST,DIV HEMATOL MALIGNANCIES,BLOOOD COMPONENT LAB,BOSTON,MA 02115. RP Ohto, H (reprint author), FUKUSHIMA MED COLL,BLOOD TRANSFUS SERV,FUKUSHIMA 96012,JAPAN. NR 60 TC 35 Z9 35 U1 0 U2 0 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD FEB PY 1996 VL 36 IS 2 BP 117 EP 123 DI 10.1046/j.1537-2995.1996.36296181922.x PG 7 WC Hematology SC Hematology GA TX297 UT WOS:A1996TX29700006 PM 8614960 ER PT J AU Webb, IJ Eickhoff, CE Elias, AD Ayash, LJ Wheeler, CA Schwartz, GN Demetri, GD Anderson, KC AF Webb, IJ Eickhoff, CE Elias, AD Ayash, LJ Wheeler, CA Schwartz, GN Demetri, GD Anderson, KC TI Kinetics of peripheral blood mononuclear cell mobilization with chemotherapy and/or granulocyte-colony-stimulating factor: Implications for timing and yield of hematopoietic progenitor cell collections SO TRANSFUSION LA English DT Article ID HIGH-DOSE CHEMOTHERAPY; AUTOLOGOUS BONE-MARROW; STEM-CELLS; BREAST-CANCER; G-CSF; PLATELET RECOVERY; MACROPHAGE; TRANSPLANTATION; CYCLOPHOSPHAMIDE; LEUKAPHERESIS AB Background: Peripheral blood progenitor cells (PBPCs) are commonly collected and used to reconstitute hematopoiesis after high-dose chemotherapy. However, strategies for optimal collection and assessment of leukapheresis components are not standardized. Study Design and Methods: Hematopoietic progenitor cell assays were performed on 369 leukapheresis components collected from 95 patients who had received doxorubicin-based chemotherapy and/or granulocyte-colony-stimulating factor (G-CSF). Precollection patient hematologic values, leukapheresis collection values, component hematopoietic progenitor cell assays, and patient outcome measures were summarized. The kinetics of mononuclear cell (MNC) and PBPC mobilization were assessed among four patient groups. Results: Patient group was a significant predictor of the peripheral blood MNC count on the day of collection (p<0.0001), and that value was a significant predictor of granulocyte-macrophage-colony-forming unit (CFU-GM) yield (p<0.0001). This relationship between the peripheral blood MNC count on the day of collection and CFU-GM yield differed according to patient group (p<0.0001). CFU-GM made up a larger fraction of peripheral blood MNCs collected from patients who received chemotherapy plus G-CSF than collected from those who received G-CSF alone. Moreover, the peripheral blood MNC count and the corresponding CFU-GM yield increased significantly on consecutive days of collection in patient groups receiving chemotherapy and G-CSF but were unchanged or decreased in patients receiving G-CSF alone. Conclusion: The relationship between peripheral blood MNC count and leukapheresis component CFU-GM yield differed significantly between patients who received chemotherapy and G-CSF and those who received G-CSF alone for the mobilization of PBPCs. Patient peripheral blood MNC count and component CFU-GM yield are useful for both assessing and suggesting revisions to PBPC mobilization and collection strategies. C1 DANA FARBER CANC INST,DEPT MED,BOSTON,MA 02115. DANA FARBER CANC INST,DEPT BIOSTAT,BOSTON,MA 02115. HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DIV MED ONCOL,BOSTON,MA. RP Webb, IJ (reprint author), DANA FARBER CANC INST,DIV HEMATOL MALIGNANCIES,BLOOD COMPONENT LAB,44 BINNEY ST,D289,BOSTON,MA 02115, USA. FU NCI NIH HHS [CA 06516] NR 30 TC 20 Z9 20 U1 0 U2 0 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD FEB PY 1996 VL 36 IS 2 BP 160 EP 167 DI 10.1046/j.1537-2995.1996.36296181930.x PG 8 WC Hematology SC Hematology GA TX297 UT WOS:A1996TX29700014 PM 8614968 ER PT J AU Vamvakas, EC AF Vamvakas, EC TI Transfusion-associated cancer recurrence and postoperative infection: Meta-analysis of randomized, controlled clinical trials SO TRANSFUSION LA English DT Article ID PERIOPERATIVE BLOOD-TRANSFUSION; NATURAL-KILLER-CELLS; COLORECTAL-CANCER; MYOCARDIAL-INFARCTION; HOMOLOGOUS BLOOD; IMMUNE FUNCTION; BREAST-CANCER; SURVIVAL; SURGERY; MORTALITY C1 HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. RP Vamvakas, EC (reprint author), MASSACHUSETTS GEN HOSP,BLOOD TRANSFUS SERV,DEPT PATHOL,GRJ-224,GRAY BLDG,FRUIT ST,BOSTON,MA 02114, USA. NR 84 TC 142 Z9 146 U1 0 U2 2 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 SN 0041-1132 J9 TRANSFUSION JI Transfusion PD FEB PY 1996 VL 36 IS 2 BP 175 EP 186 DI 10.1046/j.1537-2995.1996.36296181932.x PG 12 WC Hematology SC Hematology GA TX297 UT WOS:A1996TX29700016 PM 8614970 ER PT J AU Shipp, TD Bromley, B Pauker, S Frigoletto, FD Benacerraf, BR AF Shipp, TD Bromley, B Pauker, S Frigoletto, FD Benacerraf, BR TI Outcome of singleton pregnancies with severe oligohydramnios in the second and third trimesters SO ULTRASOUND IN OBSTETRICS & GYNECOLOGY LA English DT Article DE oligohydramnios; second and third trimesters; singletons; outcome ID SERUM ALPHA-FETOPROTEIN; AMNIOTIC-FLUID VOLUME; INTRAUTERINE GROWTH-RETARDATION; ULTRASOUND EVALUATION; 2ND-TRIMESTER OLIGOHYDRAMNIOS; EXPECTANT MANAGEMENT; PREDICTIVE VALUE; MIDTRIMESTER; MEMBRANES; RUPTURE AB We evaluated the significance of severe oligohydramnios, or anhydramnios, in the second and third trimesters, by determining the range of etiologies as well as the differences in fetal and neonatal outcome. All prenatal ultrasound results on pregnancies found to have severe oligohydramnios over a 7.5-year period at 13-42 weeks' gestation were retrospectively collected. Follow-up results were obtained from review of medical records, autopsies and pathology reports. A total of 250 singleton pregnancies met the criteria of having severe oligohydramnios. A bimodal distribution in gestational age at diagnosis was seen, with move cases diagnosed at 13-21 weeks and at 34-42 weeks. Fetal abnormalities were present in 50.7% of those diagnosed with severe oligohydramnios in the second trimester and in 22.1% of those in the third trimester. There were 10.2% and 85.3% survivors when severe oligohydramnios was diagnosed in the second and third trimesters, respectively. The rate of aneuploidy was at least 4.4% for the entire singleton population. A bimodal distribution of pregnancies presenting with severe oligohydramnios represents two different naturally occurring populations in terms of both etiology and prognosis. C1 MASSACHUSETTS GEN HOSP,DEPT OBSTET & GYNECOL,BOSTON,MA 02114. NR 23 TC 11 Z9 13 U1 0 U2 0 PU PARTHENON PUBLISHING GROUP PI CARNFORTH LANCASHIRE PA CASTERTON HALL, CARNFORTH LANCASHIRE, ENGLAND LA6 2LA SN 0960-7692 J9 ULTRASOUND OBST GYN JI Ultrasound Obstet. Gynecol. PD FEB PY 1996 VL 7 IS 2 BP 108 EP 113 DI 10.1046/j.1469-0705.1996.07020108.x PG 6 WC Acoustics; Obstetrics & Gynecology; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Obstetrics & Gynecology; Radiology, Nuclear Medicine & Medical Imaging GA TZ152 UT WOS:A1996TZ15200008 PM 8776235 ER PT J AU McDougal, WS Perlmutter, AD AF McDougal, WS Perlmutter, AD TI Securing certification by the American Board of Urology SO UROLOGY LA English DT Editorial Material C1 WAYNE STATE UNIV,SCH MED,DEPT PEDIAT UROL,DETROIT,MI. HARVARD UNIV,SCH MED,DEPT SURG,CAMBRIDGE,MA 02138. RP McDougal, WS (reprint author), MASSACHUSETTS GEN HOSP,UROL SERV,DEPT UROL,GRB 1102,32 FRUIT ST,BOSTON,MA 02114, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD FEB PY 1996 VL 47 IS 2 BP 157 EP 158 PG 2 WC Urology & Nephrology SC Urology & Nephrology GA TW787 UT WOS:A1996TW78700002 PM 8607226 ER PT J AU Zietman, AL Dallow, KC McManus, PA Heney, NM Shipley, WU AF Zietman, AL Dallow, KC McManus, PA Heney, NM Shipley, WU TI Time to second prostate-specific antigen failure is a surrogate endpoint for prostate cancer death in a prospective trial of therapy for localized disease SO UROLOGY LA English DT Article ID TOTAL ANDROGEN ABLATION; EXPERIENCE; CARCINOMA AB Objectives. The most relevant endpoint in comparing the efficacy of curative therapies for prostate cancer is cancer-specific death. Prospective trials need to mature for at least a decade to yield meaningful cancer death data due to the long natural history of the disease and the use of salvage androgen suppression, This delay may be long enough that the tested treatments are outdated by the time of reporting; thus, there is a need for reliable early surrogate endpoints for cancer survival. Methods. This report evaluates 202 patients entered into a single institution prospective randomized study for T3-4 prostate cancer. Patients were accrued between 1982 and 1992 and received radical irradiation to either a standard dose of 67.2 Gy or a higher dose of 75.6 Gy. Median follow-up was 5.4 years. A total of 76 men have received androgen suppression or orchiectomy for salvage following relapse. Of this group, 35 experienced a second relapse heralded by a rise in the serum prostate-specific antigen (PSA). Results. The median survival from the time of second biochemical relapse (defined as a progression with a rise in serum PSA more than 10% above the nadir after androgen suppression) was 27 months. Kaplan-Meier analysis projected a 0% survival for this group at 4 years, All those dying after second biochemical failure died of the prostate cancer. Conclusions. Second PSA failure (or PSA progression on hormonal therapy) has potential as a surrogate for impending cancer death and its use as an endpoint in prospective studies could allow earlier reporting by 2 to 4 years. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CTR CANC,DEPT UROL,BOSTON,MA 02114. RP Zietman, AL (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CTR CANC,DEPT RADIAT ONCOL,BOSTON,MA 02114, USA. NR 13 TC 35 Z9 35 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0090-4295 J9 UROLOGY JI UROLOGY PD FEB PY 1996 VL 47 IS 2 BP 236 EP 239 DI 10.1016/S0090-4295(99)80423-3 PG 4 WC Urology & Nephrology SC Urology & Nephrology GA TW787 UT WOS:A1996TW78700020 PM 8607241 ER PT J AU Corleto, VD Scopinaro, F Angeletti, S Materia, A Basso, N Polettini, E Annibale, B Schillaci, O DAmbra, G Marignani, M Gualdi, G Bordi, C Passaro, EJ DelleFave, G AF Corleto, VD Scopinaro, F Angeletti, S Materia, A Basso, N Polettini, E Annibale, B Schillaci, O DAmbra, G Marignani, M Gualdi, G Bordi, C Passaro, EJ DelleFave, G TI Somatostatin receptor localization of pancreatic endocrine tumors SO WORLD JOURNAL OF SURGERY LA English DT Article ID ZOLLINGER-ELLISON SYNDROME; INVIVO APPLICATION; POSITIVE TUMORS; SCINTIGRAPHY; -OCTREOTIDE; ANALOG AB Gastroenteropancreatic endocrine tumors are difficult to localize. At the same time the tumor is localized, though, there is an opportunity for cure or to remove tumor tissue. In this study we have prospectively examined the ability of In-111-octreotide scintigraphy, magnetic resonance imaging (MRI), and computed tomography (CT) to localize tumor lesions in 24 patients with a biochemical or histologic diagnosis of neuroendocrine tumor. In eight patients a surgical assessment of the imaging results was prospectively performed. Planar and abdominal single-photon emission tomography (SPET) images acquired 4 and 24 hours after 180 to 220 MBq of In-111-octreotide injection were evaluated and compared with conventional imaging techniques. SPET scintigraphy visualized more presumed tumor lesions (n = 39) than conventional imaging studies (MRT, n = 25; CT, n = 13); 23 of 24 patients had positive octreotide scintigraphy, 17 of 24 had positive MRI-scans, and 12 of 24 patients had positive CT scans. It was concluded that In-111-octreotide scintigraphy combined with conventional imaging improves the preoperative localization of presumably tumorous lesions in patients with gastroenterohepatic endocrine tumors. C1 UNIV ROMA LA SAPIENZA,POLICLIN UMBERTO 1,MED CLIN 2,DEPT GASTROENTEROL,I-00161 ROME,ITALY. UNIV ROMA LA SAPIENZA,POLICLIN 1,MED CLIN 2,DEPT SURG,I-00161 ROME,ITALY. UNIV ROMA LA SAPIENZA,POLICLIN 1,MED CLIN 1,DEPT RADIOL,I-00161 ROME,ITALY. UNIV PARMA,DEPT HUMAN PATHOL,I-43100 PARMA,ITALY. W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG,LOS ANGELES,CA 90073. OI SCHILLACI, ORAZIO/0000-0002-6176-2805; Scopinaro, Francesco/0000-0003-1924-3005 NR 18 TC 25 Z9 25 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0364-2313 J9 WORLD J SURG JI World J.Surg. PD FEB PY 1996 VL 20 IS 2 BP 241 EP 244 PG 4 WC Surgery SC Surgery GA TV920 UT WOS:A1996TV92000020 PM 8661825 ER PT J AU Blahd, WH Brown, CV Khonsary, SA Farahi, JB Quinones, N Ribe, JY Coyle, JJ Glass, EC Mandelkern, MA AF Blahd, WH Brown, CV Khonsary, SA Farahi, JB Quinones, N Ribe, JY Coyle, JJ Glass, EC Mandelkern, MA TI PET scans of abdominal malignancy SO WORLD JOURNAL OF SURGERY LA English DT Article ID COLORECTAL TUMORS; CANCER AB Positron emission tomography (PET) with fluorine-18-2-D-deoxyglucose (FDG) currently is being integrated into clinical oncology because it provides unique functional information that can be applied to the management of cancer. In particular, it is useful for assessing tumor activity and growth, evaluating efficacy of therapy, and detecting tumor recurrence. Studies have demonstrated the value of whole-body PET-FDG imaging when staging and managing abdominal malignancy. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT RADIOL SCI,LOS ANGELES,CA 90095. RP Blahd, WH (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NUCL MED SERV,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 10 TC 10 Z9 10 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0364-2313 J9 WORLD J SURG JI World J.Surg. PD FEB PY 1996 VL 20 IS 2 BP 245 EP 247 PG 3 WC Surgery SC Surgery GA TV920 UT WOS:A1996TV92000021 PM 8661826 ER PT J AU Derian, CK Solomon, HF Higgins, JD Beblavy, MJ Santulli, RJ Bridger, GJ Pike, MC Kroon, DJ Fischman, AJ AF Derian, CK Solomon, HF Higgins, JD Beblavy, MJ Santulli, RJ Bridger, GJ Pike, MC Kroon, DJ Fischman, AJ TI Selective inhibition of N-formylpeptide-induced neutrophil activation by carbamate-modified peptide analogues SO BIOCHEMISTRY LA English DT Article ID HUMAN POLYMORPHONUCLEAR LEUKOCYTES; IMAGING FOCAL SITES; CHEMOTACTIC PEPTIDES; BACTERIAL-INFECTION; RABBIT NEUTROPHILS; BINDING POCKET; RECEPTOR; CHEMOATTRACTANTS; REQUIREMENTS; ANTAGONISTS AB Stimulation Of the leukocyte N-formylpeptide receptor (FPR) induces chemotaxis, cell adhesion, free radical release, and degranulation, responses associated with infection and inflammation. Under conditions where continuous activation of the receptor prevails, neutrophil-dependent tissue damage ensues. Antagonists of the FPR have potential for use as diagnostic and therapeutic agents. Hence, we have synthesized and evaluated a series of amino-terminal carbamate analogues of the peptide Met-Leu-Phe (MLF) in order to determine the structural requirements for imparting agonist or antagonist activity at the human neutrophil FPR, Peptides were evaluated in three in vitro assays: receptor binding, superoxide anion release, and cell adhesion. Unbranched carbamates (methoxycarbonyl, ethoxycarbonyl, and n-butyloxycarbonyl) resulted in agonist activity, whereas branched carbamates (iso-butyloxycarbonyl, tert-butyloxycarbonyl, and benzyloxycarbonyl) were antagonists. The peptide antagonists were more potent inhibitors of superoxide anion release than cell adhesion by 4-7-fold. When iso-butyloxycarbonyl-MLF (i-Boc-MLF) was further modified at the carboxy terminus with Lys, antagonist potency was retained but without functional selectivity. Further C-terminal modification with the radionuclide linker diethylenetriaminepentaacetic acid did not alter the potency of i-Boc-MLFK. These results indicate that the switch from agonist to antagonist activity can be achieved by modifying the overall size and shape of the aminoterminal group; that modifications at both the amino and carboxy termini can alter the functional selectivity of the peptide; and that modifications can be tolerated at the carboxy terminus to allow for development of an antagonist for diagnostic applications. C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. JOHNSON MATTHEY BIOMED RES,W CHESTER,PA 19380. RP Derian, CK (reprint author), RW JOHNSON PHARMACEUT RES INST,SPRING HOUSE,PA 19477, USA. FU NIAID NIH HHS [R01 AI31094] NR 31 TC 49 Z9 49 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD JAN 30 PY 1996 VL 35 IS 4 BP 1265 EP 1269 DI 10.1021/bi952087k PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TT375 UT WOS:A1996TT37500020 PM 8573582 ER PT J AU Podgor, MJ Hiller, R AF Podgor, MJ Hiller, R TI Associations of types of lens opacities between and within eyes of individuals: An application of second-order generalized estimating equations SO STATISTICS IN MEDICINE LA English DT Article ID CORRELATED BINARY REGRESSION; ODDS-RATIO; MODELS; CATARACTS; DISEASE AB The lens opacity characteristics of individuals constitute multivariate data. Our goal was to estimate the associations between the three main types of age-related lens opacities (nuclear, cortical, posterior subcapsular) both between and within eyes of individuals using cross-sectional data from the Framingham (Massachusetts) Eye Studies. We describe use of a recently proposed extension of the generalized estimating equations approach to marginal logistic models (GEE2), and we demonstrate that a variety of research problems can be investigated with this methodology. For example, in our data, there were strong associations of the same opacity types between the two eyes of individuals and weak associations between different types of opacities. We also note that estimation of such associations may be limited in other epidemiologic settings. C1 BOSTON UNIV,BOSTON,MA 02215. JOSLIN DIABET CTR,BOSTON,MA 02215. NHLBI,FRAMINGHAM,MA. RP Podgor, MJ (reprint author), NEI,DIV BIOMETRY & EPIDEMIOL,BLDG 31,ROOM 6A52,31 CTR DR,MSC 2510,BETHESDA,MD 20892, USA. FU NEI NIH HHS [N01-EY-9-2109, N01-EY-6-2105] NR 27 TC 9 Z9 9 U1 0 U2 0 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX, ENGLAND PO19 1UD SN 0277-6715 J9 STAT MED JI Stat. Med. PD JAN 30 PY 1996 VL 15 IS 2 BP 145 EP 156 DI 10.1002/(SICI)1097-0258(19960130)15:2<145::AID-SIM150>3.0.CO;2-1 PG 12 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA TP247 UT WOS:A1996TP24700003 PM 8614751 ER PT J AU Benson, TE Berglund, AM Brown, MC AF Benson, TE Berglund, AM Brown, MC TI Synaptic input to cochlear nucleus dendrites that receive medial olivocochlear synapses SO JOURNAL OF COMPARATIVE NEUROLOGY LA English DT Article DE vesicle; intensity coding; efferent; descending system; collateral ID AUDITORY-NERVE FIBERS; SUPERIOR OLIVARY COMPLEX; GUINEA-PIG; ELECTRICAL-STIMULATION; BRAIN-STEM; INFERIOR COLLICULUS; FINE-STRUCTURE; CAT; NEURONS; CELLS AB Axons of olivocochlear neurons originate in the superior olivary complex and project to the cochlea. Along their course, medial olivocochlear axons give off branches to the cochlear nucleus. We labeled these branches with horseradish peroxidase and used electron microscopy to determine their target dendrites. Target dendrites were of two classes: ''large'' dendrites and ''varicose'' dendrites. Using serial sections, we reconstructed the dendrites and, in addition to the labeled olivocochlear input, we determined the synaptic profile of unlabeled inputs onto the dendrites. We classified the terminals on the basis of the shape and size of their synaptic vesicles. On large dendrites, the predominant type of unlabeled terminal had small round (SmRnd) vesicles. These terminals are likely to be excitatory, and some of them may originate from unlabeled medial olivocochlear branches. On varicose dendrites, the predominant type of terminal had pleomorphic vesicles. These terminals are likely to be inhibitory. They may be from descending inputs that arise in higher centers. A final type of terminal onto large dendrites exhibited signs of neuronal degeneration, possibly because the cell body of origin was damaged during the injection procedure. These terminals often had long, perforated synaptic densities and may originate from type II primary afferents. Thus, medial olivocochlear efferents and type II afferents, which both contact outer hair cells in the periphery, appear to synapse onto the same targets in the cochlear nucleus. In contrast, where examined, the target dendrites did not receive terminals with large vesicles from afferents that contact inner hair cells. Thus, target neurons appear to function in a neural circuit associated more closely with outer than with inner hair cells. (C) 1996 Wiley-Liss, Inc. C1 MASSACHUSETTS EYE & EAR INFIRM, EATON PEABODY LAB, BOSTON, MA 02114 USA. BOSTON UNIV, DEPT BIOMED ENGN, BOSTON, MA 02215 USA. HARVARD UNIV, SCH MED, DEPT OTOL & LARYNGOL, BOSTON, MA 02114 USA. HARVARD MIT DIV HLTH SCI & TECHNOL, BOSTON, MA 02114 USA. FU NIDCD NIH HHS [DC00119, DC01089, DC00006] NR 72 TC 40 Z9 40 U1 0 U2 0 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0021-9967 J9 J COMP NEUROL JI J. Comp. Neurol. PD JAN 29 PY 1996 VL 365 IS 1 BP 27 EP 41 PG 15 WC Neurosciences; Zoology SC Neurosciences & Neurology; Zoology GA TU049 UT WOS:A1996TU04900003 PM 8821439 ER PT J AU Thadhani, R Pascual, M Nickeleit, V TolkoffRubin, N Colvin, R AF Thadhani, R Pascual, M Nickeleit, V TolkoffRubin, N Colvin, R TI Preliminary description of focal segmental glomerulosclerosis in patients with renovascular disease SO LANCET LA English DT Article ID PROTEINURIA AB Background Primary and secondary forms of focal segmental glomerulosclerosis (FSGS) are common causes of glomerular proteinuria. Secondary forms of FSGS seem to be the result of adaptive changes that follow a reduction in renal mass. We saw an elderly patient with severe bilateral renal vascular disease (RVD) who had FSGS on percutaneous biopsy. To find out whether elderly patients with atherosclerotic RVD are predisposed to the development of FSGS, we reviewed all cases of FSGS at our institution between 1990 and 1995. Methods We identified 59 cases of biopsy-proven FSGS and examined clinical, histological, and radiographic records. Findings Of the 59 patients, 24 were older than 50 years; eight of these had RVD. No patient under the age of 50 had RVD. Seven of the eight patients with RVD and FSGS had substantial proteinuria at presentation. All had typical glomerular lesions with focal segmental tuft collapse and synechiae; other glomeruli were hypertrophic. All patients showed further decline in renal function on follow-up. Interpretation The association of FSGS and RVD may represent an under-recognised aetiology of significant proteinuria in elderly patients. C1 MASSACHUSETTS GEN HOSP,RENAL UNIT,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. NR 10 TC 54 Z9 54 U1 0 U2 1 PU LANCET LTD PI LONDON PA 42 BEDFORD SQUARE, LONDON, ENGLAND WC1B 3SL SN 0140-6736 J9 LANCET JI Lancet PD JAN 27 PY 1996 VL 347 IS 8996 BP 231 EP 233 DI 10.1016/S0140-6736(96)90406-7 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA TT069 UT WOS:A1996TT06900012 PM 8551883 ER PT J AU Eason, JD Pascual, M Wee, S Farrell, M Phelan, J Boskovic, S Blosch, C Mohler, KM Cosimi, AB AF Eason, JD Pascual, M Wee, S Farrell, M Phelan, J Boskovic, S Blosch, C Mohler, KM Cosimi, AB TI Evaluation of recombinant human soluble dimeric tumor necrosis factor receptor for prevention of OKT3-associated acute clinical syndrome SO TRANSPLANTATION LA English DT Article; Proceedings Paper CT 21st Annual Meeting of the American-Society-of-Transplant-Surgeons CY MAY 17-19, 1995 CL CHICAGO, IL SP Amer Soc Transplant Surgeons ID OKT3 MONOCLONAL-ANTIBODY; KIDNEY-TRANSPLANT RECIPIENTS; RENAL-ALLOGRAFT REJECTION; FACTOR-ALPHA; GAMMA-INTERFERON; SERUM LEVELS; TNF-ALPHA; LYMPHOTOXIN; NEPHROTOXICITY; INTERLEUKIN-2 AB Tumor necrosis factor alpha (TNFa) has been shown to be the primary cytokine responsible for the OKT3-induced acute clinical syndrome (OKT3-ACS). Recombinant human soluble tumor necrosis factor receptor (TNFR:Fc) is a dimer of the p80 TNF receptor, which binds both TNFa and lymphotoxin (LT), Renal allograft recipients undergoing OKT3 therapy for steroid-resistant rejection were randomized to receive OKT3 alone or in combination with TNFR:Fc to determine its safety and efficacy in decreasing the severity of OKT3-ACS and in restoring renal function, Six of 12 patients were given TNFR:Fc prior to each of the first two injections of OKT3. All patients were monitored for manifestations of OKT3-ACS and changes in renal function. In addition, serial serum samples were assayed for TNFa and TNFR:Fc levels (ELISA) and TNFa bioactivity (L929). No adverse side effects were identified in patients receiving TNFR:Fc. Patients treated with TNFR:Fc had significantly fewer symptoms by day 2 of OKT3, and had a lower overall incidence of chills and arthralgias. Renal dysfunction reversed within 24 hr in the TNFR:Fc-treated group in contrast to the 48-72-hr delay in the control group, Antigenic TNFa levels increased in the control group from <10 pg/ml pre OKT3 to a mean peak level of 30+/-13 pg/ml on day 1 and decreased to pretreatment levels by day 2, TNFR:Fc-treated patients had a mean peak TNFa level of 235+/-135 pg/ml, suggesting a carrier effect of TNFR: Fc, In contrast, bioactivity was barely detectable (mean 20+/-14 pg/ml) in the day 1 samples from TNFR: Fc-treated patients, whereas significant bioactivity (peak mean 60+/-35 pg/ml) was detected in sera from control patients, TNF receptor levels reached 600 ng/ml in treated patients and remained elevated for up to 18 days confirming the long half-life of TNFR:Fc, This phase 1 trial demonstrates that TNFR:Fc is well tolerated and may limit the severity of OKT3-ACS, The most significant observation was a more rapid improvement in renal function in the TNFR:Fc-treated patients. The absence of TNFa bioactivity indicates that TNFR:Fc functions as a TNF antagonist. Further evaluation of higher doses of TNFR:Fc in OKT3-treated patients is currently in progress. C1 MASSACHUSETTS GEN HOSP,GEN SURG SERV,TRANSPLANTAT UNIT,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT SURG,BOSTON,MA 02114. IMMUNEX RES & DEV CORP,SEATTLE,WA 98101. NR 31 TC 36 Z9 37 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD JAN 27 PY 1996 VL 61 IS 2 BP 224 EP 228 DI 10.1097/00007890-199601270-00011 PG 5 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA TT970 UT WOS:A1996TT97000011 PM 8600628 ER PT J AU Washburn, WK Bradley, J Cosimi, AB Freeman, RB Hull, D Jenkins, RL Lewis, WD Lorber, MI Schweizer, RT Vacanti, JP Rohrer, RJ AF Washburn, WK Bradley, J Cosimi, AB Freeman, RB Hull, D Jenkins, RL Lewis, WD Lorber, MI Schweizer, RT Vacanti, JP Rohrer, RJ TI A regional experience with emergency liver transplantation SO TRANSPLANTATION LA English DT Article; Proceedings Paper CT 21st Annual Meeting of the American-Society-of-Transplant-Surgeons CY MAY 17-19, 1995 CL CHICAGO, IL SP Amer Soc Transplant Surgeons ID RETRANSPLANTATION; ALLOCATION; SURVIVAL AB Liver transplantation for patients requiring life-support results in the lowest survival and highest costs. A ten year (1983-1993) regional experience with liver transplantation for critically ill patients was undertaken to ascertain the fate of several subgroups of patients, Of the 828 liver transplants performed at six transplant centers within the region over this period, 168 (20%) were done in patients who met today's criteria for a United Network of Organ Sharing (UNOS) status 1 (emergency) liver transplant candidate. Recipients were classified according to chronicity of disease and transplant number (primary-acute, primary-chronic, reTx-acute, reTx-chronic). Overall one-year survival was 50% for all status 1 recipients. The primary-acute subgroup (n=63) experienced a 57% one-year survival compared with 50% for the primary-chronic (n=51) subgroup (P=0.07). Of the reTx-acute recipients (n=43), 44% were alive at one year in comparison with 20% for the reTx-chronic (n=11) group (P=0.18), There was no significant difference in survival for the following: transplant center, blood group compatibility with donors, age, preservation solution, or graft size. For patients retransplanted for acute reasons (primary graft nonfunction (PGNF) or hepatic artery thrombosis [HAT]), survival was significantly better if a second donor was found within 3 days of relisting (52% vs, 20%; P=0.012). Over the study period progressively fewer donor organs came from outside the region. No strong survival-based argument can be made for separating, in allocation priority, acute and chronic disease patients facing the first transplant as a status 1 recipient. Clearly patients suffering from PONE or HAT do far better if retransplanted within 3 days. Establishing an even higher status for recipients with PGNF, perhaps drawing from a supraregional donor pool, would allow surgeons to accept more marginal donors, thus potentially expanding the pool, without significantly compromising patient survival. Retransplantation of the recipient with a chronically failing graft who deteriorates to the point of needing life-support is nearly futile, and in today's health care climate, not an optimal use of scarce donor livers. C1 MASSACHUSETTS GEN HOSP,TUFTS NEW ENGLAND MED CTR,NEW ENGLAND DEACONESS HOSP,DEPT TRANSPLANTAT,BOSTON,MA 02111. TUFTS UNIV,NEW ENGLAND MED CTR,DEPT SURG,BOSTON,MA 02111. CHILDRENS HOSP,BOSTON,MA. HARTFORD HOSP,HARTFORD,CT 06115. YALE NEW HAVEN MED CTR,NEW HAVEN,CT 06504. NEW ENGLAND ORGAN BANK INC,NEWTON,MA. RI Washburn, William/Q-5677-2016 NR 15 TC 15 Z9 15 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD JAN 27 PY 1996 VL 61 IS 2 BP 235 EP 239 DI 10.1097/00007890-199601270-00013 PG 5 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA TT970 UT WOS:A1996TT97000013 PM 8600630 ER PT J AU Li, YP Chen, W Stashenko, P AF Li, YP Chen, W Stashenko, P TI Molecular cloning and characterization of a putative novel human osteoclast-specific 116-kDa vacuolar proton pump subunit SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID H+-ATPASE; ADENOSINE-TRIPHOSPHATASE; BONE-RESORPTION; A-SUBUNIT; GENES; PROTEIN; CELL; EXPRESSION; ISOFORMS; ENCODES AB A cDNA encoding a possible novel human 116-kDa polypeptide subunit of the osteoclastic proton pump (OC-116KDa) has been identified by differential screening of a human osteoclastoma cDNA library. The predicted sequence of OC-116KDa consists of 822 amino acids and is 46.9% and 47.2% identical at the amino acid level to the 116-KDa polypeptide of the vacuolar proton pump of rat and bovine brain respectively. OC-116KDa mRNA was found at high levels in osteoclastomas by Northern analysis but was not detected in tumor stromal cells or in other tissues including kidney, liver, skeletal muscle and brain. OC-116KDa mRNA was localized to multinucleated giant cells within the osteoclastoma tumor by in situ hybridization. (C) 1996 Academic Press, Inc. RP Li, YP (reprint author), FORSYTH DENT CTR,DEPT CYTOKINE BIOL,140 FENWAY,BOSTON,MA 02115, USA. FU NIDCR NIH HHS [DE-07378] NR 38 TC 77 Z9 83 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD JAN 26 PY 1996 VL 218 IS 3 BP 813 EP 821 DI 10.1006/bbrc.1996.0145 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA TT598 UT WOS:A1996TT59800031 PM 8579597 ER PT J AU Wilson, CJ Chao, DM Imbalzano, AN Schnitzler, GR Kingston, RE Young, RA AF Wilson, CJ Chao, DM Imbalzano, AN Schnitzler, GR Kingston, RE Young, RA TI RNA polymerase II holoenzyme contains SWI/SNF regulators involved in chromatin remodeling SO CELL LA English DT Article ID SACCHAROMYCES-CEREVISIAE; BINDING-PROTEIN; TRANSCRIPTIONAL ACTIVATORS; NEGATIVE REGULATOR; SNF6 PROTEINS; YEAST; GENES; EXPRESSION; SNF2/SWI2; DNA AB The RNA polymerase II holoenzyme contains RNA polymerase II, a subset of general transcription factors and SRB regulatory proteins. We report here that SWI and SNF gene products, previously identified as global gene regulators whose functions include remodeling chromatin, are also integral components of the yeast RNA polymerase II holoenzyme. The SWI/SNF proteins are components of the SRB complex, also known as the mediator, which is tightly associated with the RNA polymerase II C-terminal repeat domain. The SWI/SNF components provide the holoenzyme with the capacity to disrupt nucleosomal DNA and thus facilitate stable binding of various components of the transcription initiation complex at promoters. C1 MIT,DEPT BIOL,CAMBRIDGE,MA 02139. MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115. RP Wilson, CJ (reprint author), WHITEHEAD INST BIOMED RES,9 CAMBRIDGE CTR,CAMBRIDGE,MA 02142, USA. RI Young, Richard/F-6495-2012 OI Young, Richard/0000-0001-8855-8647 NR 57 TC 319 Z9 321 U1 1 U2 6 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 0092-8674 J9 CELL JI Cell PD JAN 26 PY 1996 VL 84 IS 2 BP 235 EP 244 DI 10.1016/S0092-8674(00)80978-2 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA TR595 UT WOS:A1996TR59500010 PM 8565069 ER PT J AU Bharti, AK Olson, MOJ Kufe, DW Rubin, EH AF Bharti, AK Olson, MOJ Kufe, DW Rubin, EH TI Identification of a nucleolin binding site in human topoisomerase I SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SELF-CLEAVING ACTIVITY; RNA POLYMERASE-I; DNA TOPOISOMERASES; RIBOSOMAL-RNA; MONOCLONAL-ANTIBODIES; FISSION YEAST; TRANSCRIPTION; CELLS; ASSOCIATION; TEMPLATE AB DNA topoisomerase I (topo I) is involved in the regulation of DNA supercoiling, gene transcription, and rDNA recombination. However, little is known about interactions between topo I and other nuclear proteins. We used affinity chromatography with a topo I fusion protein to screen U-937 leukemic cell extracts and have identified nucleolin as a topo I-binding protein. Coimmunoprecipitation and other studies demonstrate that the interaction between topo I and nucleolin is direct. Furthermore, deletion analyses have identified the 166-210-amino acid region of topo I as sufficient for the interaction with nucleolin, Since nucleolin has been implicated in nuclear transport and in a variety of transcriptional processes, the interaction with topo I may relate to the cellular localization of topo I or to the known role of this topoisomerase in transcription. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV CANC PHARMACOL,BOSTON,MA 02115. UNIV MISSISSIPPI,MED CTR,DEPT BIOCHEM,JACKSON,MS 39216. FU NCI NIH HHS [CA70981, CA19589]; NIGMS NIH HHS [GM28349] NR 40 TC 97 Z9 99 U1 1 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 26 PY 1996 VL 271 IS 4 BP 1993 EP 1997 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TR320 UT WOS:A1996TR32000031 PM 8567649 ER PT J AU Tang, ZL Scherer, PE Okamoto, T Song, K Chu, C Kohtz, DS Nishimoto, I Lodish, HF Lisanti, MP AF Tang, ZL Scherer, PE Okamoto, T Song, K Chu, C Kohtz, DS Nishimoto, I Lodish, HF Lisanti, MP TI Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID TRANS-GOLGI-NETWORK; GROWTH FACTOR-II; PLASMA-MEMBRANE; SKELETAL-MUSCLE; SMOOTH-MUSCLE; PROTEIN; RECEPTOR; COMPONENT; VESICLES; CELLS AB Caveolin, a 21-24-kDa integral membrane protein, is a principal component of caveolar membranes in vivo. Caveolin interacts directly with heterotrimeric G-proteins and can functionally regulate their activity. Recently, a second caveolin gene has been identified and termed caveolin-2. Here, we report the molecular cloning and expression of a third member of the caveolin gene family, caveolin-3. Caveolin-3 is most closely related to caveolin-1 based on protein sequence homology; caveolin-1 and caveolin 3 are similar to 65% identical and similar to 85% similar. A single stretch of eight amino acids (FEDVIAEP) is identical in caveolin-1, -2, and -3. This conserved region may represent a ''caveolin signature sequence'' that is characteristic of members of the caveolin gene family. Caveolin-3 mRNA is expressed predominantly in muscle tissue types (skeletal muscle, diaphragm, and heart) and is selectively induced during the differentiation of skeletal C2C12 myoblasts in culture. In many respects, caveolin-3 is similar to caveolin-1: (i) caveolin-3 migrates in velocity gradients as a high molecular mass complex; (ii) caveolin-3 colocalizes with caveolin-1 by immunofluorescence microscopy and cell fractionation studies; and (iii) a caveolin-3-derived polypeptide functionally suppresses the basal GTPase activity of purified heterotrimeric G-proteins. Identification of a muscle-specific member of the caveolin gene family may have implications for understanding the role of caveolin in different muscle cell types (smooth, cardiac, and skeletal) as previous morphological studies have demonstrated that caveolae are abundant in these cells. Our results also suggest that other as yet unknown caveolin family members are likely to exist and may be expressed in a regulated or tissue-specific fashion. C1 WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142. HARVARD UNIV,SHRINERS HOSP CRIPPLED CHILDREN,MASSACHUSETTS GEN HOSP,SCH MED,DEPT ANESTHESIA,BOSTON,MA 02114. MT SINAI SCH MED,DEPT PATHOL,NEW YORK,NY 10029. MASSACHUSETTS GEN HOSP EAST,CARDIOVASC RES CTR,DEPT MED,BOSTON,MA 02129. MIT,DEPT BIOL,CAMBRIDGE,MA 02139. RI Lisanti, Michael/C-6866-2013 FU NIDDK NIH HHS [DK-47618]; NIGMS NIH HHS [GM-49516, GM-50443] NR 60 TC 524 Z9 532 U1 0 U2 12 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 26 PY 1996 VL 271 IS 4 BP 2255 EP 2261 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TR320 UT WOS:A1996TR32000069 PM 8567687 ER PT J AU Bernhardt, TG Cannistraro, PA Bird, DA Doyle, KM Laposata, M AF Bernhardt, TG Cannistraro, PA Bird, DA Doyle, KM Laposata, M TI Purification of fatty acid ethyl esters by solid-phase extraction and high-performance liquid chromatography SO JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS LA English DT Article DE solid-phase extraction; fatty acid ethyl esters ID ETHANOL; METABOLITES; CELLS AB We have developed a two-step method to purify fatty acid ethyl esters (FAEE) using solid-phase extraction (SPE), with a recovery of 70+/-3% (mean+/-S.E.M.) as assessed using ethyl oleate as a recovery marker from a standard lipid mixture in hexane. The first step of the SPE procedure involves application of a lipid mixture to an aminopropyl-silica column with simultaneous elution of FAEE and cholesteryl esters from the column with hexane. Gas chromatographic analysis of FAEE without interference from cholesteryl esters may be performed using the eluate from the aminopropyl-silica column, thus eliminating the need for an octadecylsilyl (ODS) column in this case. The FAEE can then be separated from the cholesteryl esters, if necessary, by chromatography on an ODS column and elution with isopropanol-water (5:1, v/v). Both the aminopropyl-silica and ODS columns were found to be effective for up to four uses. To permit isolation of specific FAEE species following isolation of total FAEE by the two-step SPE method, we have also developed a purification scheme for individual FAEE by high-performance liquid chromatography (HPLC). Thus, this simple method allows for reproducible isolation of total FAEE by SPE and isolation of individual FAEE species by HPLC. C1 MASSACHUSETTS GEN HOSP, DEPT PATHOL, DIV CLIN LABS, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. NR 11 TC 52 Z9 53 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4347 J9 J CHROMATOGR B JI J. Chromatogr. B-Biomed. Appl. PD JAN 26 PY 1996 VL 675 IS 2 BP 189 EP 196 DI 10.1016/0378-4347(95)00387-8 PG 8 WC Biochemical Research Methods; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA TW151 UT WOS:A1996TW15100001 PM 8852705 ER PT J AU Weber, GF Ashkar, S Glimcher, MJ Cantor, H AF Weber, GF Ashkar, S Glimcher, MJ Cantor, H TI Receptor-ligand interaction between CD44 and osteopontin (Eta-1) SO SCIENCE LA English DT Article ID T-CELL ACTIVATION; SECRETED PHOSPHOPROTEIN; OSTEO-SARCOMA; BONE; LINES; IMMUNOLOCALIZATION; ANTIBODIES; DEFINITION; EXPRESSION; PROTEIN AB The CD44 family of surface receptors regulates adhesion, movement, and activation of normal and neoplastic cells. The cytokine osteopontin (Eta-1), which regulates similar cellular functions, was found to be a protein ligand of CD44. Osteopontin induces cellular chemotaxis but not homotypic aggregation, whereas the inverse is true for the interaction between CD44 and a carbohydrate ligand, hyaluronate. The different responses of cells after CD44 ligation by either osteopontin or hyaluronate may account for the independent effects of CD44 on cell migration and growth. This mechanism may also be exploited by tumor cells to promote metastasis formation. C1 HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT ORTHOPED RES,BOSTON,MA 02115. RP Weber, GF (reprint author), HARVARD UNIV,SCH MED,DEPT PATHOL,DANA FARBER CANC INST,DIV IMMUNOPATHOL,BOSTON,MA 02115, USA. FU NIAID NIH HHS [AI12184, AI13600]; NIAMS NIH HHS [P01 AR34078] NR 37 TC 630 Z9 657 U1 1 U2 13 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 SN 0036-8075 J9 SCIENCE JI Science PD JAN 26 PY 1996 VL 271 IS 5248 BP 509 EP 512 DI 10.1126/science.271.5248.509 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TR322 UT WOS:A1996TR32200043 PM 8560266 ER PT J AU Martinez, V Coy, DH Lloyd, KCK Tache, Y AF Martinez, V Coy, DH Lloyd, KCK Tache, Y TI Intracerebroventricular injection of somatostatin sst(5) receptor agonist inhibits gastric acid secretion in rats SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE somatostatin sst(2) receptor; somatostatin sst(3) receptor; somatostatin sst(5) receptor; SMS 201-995; somatostatin receptor subtype; gastric acid secretion; brain; somatostatin analog; (rat) ID CENTRAL NERVOUS-SYSTEM; FUNCTIONAL EXPRESSION; CONTAINING NEURONS; MOLECULAR-CLONING; SUBTYPE; SMS-201-995; AFFINITY; RELEASE; ANALOG; BRAIN AB Somatostatin and its analogs act in the brain to influence gastric acid secretion. Five different somatostatin receptor subtypes have been characterized (sst(1) to sst(5)). We studied the influence of somatostatin (0.18-0.6 nmol/rat) and selective sst,, sst, and sst, receptor ligands on basal gastric acid secretion in conscious rats equipped with chronic gastric and intracereb roventricular (i.c.v.) cannulae. Somatostatin-14 (0.36 nmol/rat), the sst(2), sst(3) and sst(5) receptor agonist, Des-AA(1,2,4,5,12,13)-[D-Tryp(8),D-Cys(14)]somatostatin (SMS 201-995) (0.18-0.36 nmol/rat) and the sst, receptor agonist, BIM-23052, (0.8-1.2 nmol/rat) injected i.c.v. inhibited gastric acid secretion. Maximal inhibition reaching 42%, 60% and 42% was induced by somatostatin-14 (0.36 nmol/rat), SMS 201-995 (0.18 nmol/rat) and BIM-23052 (0.8 nmol/rat) respectively. The sst(2) receptor agonist, DC 32-87 (0.2-0.8 nmol/rat) and sst, receptor agonist, BIM-23056 (0.2-1.2 nmol/rat), did not modify gastric acid secretion, except the sst(3) receptor agonist at 0.4 nmol/rat which increased acid output at 20 min post-injection. The sst(2) receptor agonists (0.4 nmol/rat) co-injected i.c.v with a subthreshold dose of sst(5) (0.4 nmol/rat) inhibited gastric acid secretion. These results show that i.c.v. injection of somatostatin-14 inhibits basal gastric acid secretion in conscious rats through an action on sst(5) receptor subtype which can be potentiated by sst(2) receptor subtype. C1 UNIV CALIF LOS ANGELES,BRAIN RES INST,LOS ANGELES,CA 90073. TULANE UNIV,DEPT MED,PEPTIDE RES LABS,NEW ORLEANS,LA 70112. RP Martinez, V (reprint author), UNIV CALIF LOS ANGELES,CURE,DIGEST DIS RES CTR,W LOS ANGELES VET AFFAIRS MED CTR,DEP MED,LOS ANGELES,CA 90073, USA. RI Martinez, Vicente/N-1189-2014 FU NIDDK NIH HHS [DK-30110, DK-41301]; NIMH NIH HHS [MH-00663] NR 35 TC 12 Z9 12 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD JAN 25 PY 1996 VL 296 IS 2 BP 153 EP 160 DI 10.1016/0014-2999(95)00690-7 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TU210 UT WOS:A1996TU21000005 PM 8838451 ER PT J AU Medley, QG Kedersha, N OBrien, S Tian, QS Schlossman, SF Streuli, M Anderson, P AF Medley, QG Kedersha, N OBrien, S Tian, QS Schlossman, SF Streuli, M Anderson, P TI Characterization of GMP-17, a granule membrane protein that moves to the plasma membrane of natural killer cells following target cell recognition SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE cytotoxic lymphocytes; TIA-1; GIG-1; NKG7 ID GLYCOPROTEIN; SUBUNIT; LENS; MP20 AB Cytotoxic lymphocytes are characterized by their inclusion of cytoplasmic granules that fuse with the plasma membrane following target cell recognition. We previously identified a cytotoxic granule membrane protein designated p15-TIA-1 that is immunochemically related to an RNA-recognition motif (RRM)-type RNA-binding protein designated p40-TIA-1, Although it was suggested that p15-TIA-1 might be derived from p40-TIA-1 by proteolysis, N-terminal amino acid sequencing of p15-TIA-1 immunoaffinity purified from a natural killer (NK) cell line by using monoclonal antibody (mAb) 2G9 revealed that p15-TIA-1 is identical to the deduced amino acid sequence of NKG7 and GIG-1, cDNAs isolated from NK cells and granulocyte-colony-stimulating factor-treated mononuclear cells, respectively, Epitope mapping revealed that mAb 2G9 recognizes the C terminus of p15-TIA-1 and p40-TIA-1, The deduced amino acid sequence of p15-TIA-1/NKG7/GIG-1 predicts that the protein possesses four transmembrane domains, and immune-electron microscopy localizes the endogenous protein to the membranes of cytotoxic granules in NK cells, Given its subcellular localization, we propose to rename this protein GMP-17, for granule membrane protein of 17 kDa, Immunofluorescence microscopy of freshly isolated NK cells confirms this granular localization, Target cell-induced NK cell degranulation results in translocation of GMP-17 from granules to the plasma membrane, suggesting a possible role for GMP-17 in regulating the effector function of lymphocytes and neutrophils. C1 HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. IMMUNOGEN,CAMBRIDGE,MA 02139. RP Medley, QG (reprint author), DANA FARBER CANC INST,DIV TUMOR IMMUNOL,44 BINNEY ST,BOSTON,MA 02115, USA. FU NIAID NIH HHS [AI33600] NR 25 TC 86 Z9 91 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 23 PY 1996 VL 93 IS 2 BP 685 EP 689 DI 10.1073/pnas.93.2.685 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TR326 UT WOS:A1996TR32600028 PM 8570616 ER PT J AU Carter, RE Feldman, AR Coyle, JT AF Carter, RE Feldman, AR Coyle, JT TI Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE cancer; excitatory neurotransmission; N-acetylaspartylglutamate alpha-linked acidic dipeptidase; glutamate ID ACIDIC DIPEPTIDASE ACTIVITY; ASPARTYL-L-GLUTAMATE; AMYOTROPHIC-LATERAL-SCLEROSIS; PIG CEREBELLAR SLICES; PROTEIN-KINASE-C; N-ACETYLASPARTYLGLUTAMATE; NEUTRAL ENDOPEPTIDASE; LEUKEMIA ANTIGEN; PURKINJE-CELLS; AMINO-ACIDS AB This report demonstrates that the investigational prostatic carcinoma marker known as the prostate-specific membrane antigen (PSM) possesses hydrolytic activity with the substrate and pharmacologic properties of the N-acetylated alpha-linked acidic dipeptidase (NAALADase). NAALADase is a membrane hydrolase that has been characterized in the mammalian nervous system on the basis of its catabolism of the neuropeptide N-acetylaspartylglutamate (NAAG) to yield glutamate and N-acetylaspartate and that has been hypothesized to influence glutamatergic signaling processes, The immunoscreening of a rat brain cDNA expression library with anti-NAALADase antisera identified a 1428-base partial cDNA that shares 86% sequence identity with 1428 bases of the human PSM cDNA [Israeli, R. S., Powell, C. T., Fair, W. R, & Heston, W. D. W. (1993) Cancer Res. 53, 227-230], A cDNA containing the entire PSM open reading frame was subsequently isolated by reverse transcription-PCR from the PSM-positive prostate carcinoma cell line LNCaP, Transient transfection of this cDNA into two NAALADase-negative cell lines conferred NAAG-hydrolyzing activity that was inhibited by the NAALADase inhibitors quisqualic acid and beta-NAAG, Thus we demonstrate a PSR-encoded function and identify a NAALADase-encoding cDNA, Northern analyses identify at least six transcripts that are variably expressed in NAALADase-positive but not in NAALADase-negative rat tissues and human cell lines; therefore, PSM and/or related molecular species appear to account for NAAG hydrolysis in the nervous system, These results also raise questions about the role of PSM in both normal and pathologic prostate epithelial-cell function. C1 MASSACHUSETTS GEN HOSP EAST,DEPT PSYCHIAT,BOSTON,MA 02129. HARVARD UNIV,SCH MED,CONSOLIDATED DEPT PSYCHIAT,BOSTON,MA 02115. JOHNS HOPKINS UNIV,SCH MED,GRAD PROGRAM PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205. FU NIMH NIH HHS [MH-572901] NR 47 TC 240 Z9 245 U1 0 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 23 PY 1996 VL 93 IS 2 BP 749 EP 753 DI 10.1073/pnas.93.2.749 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TR326 UT WOS:A1996TR32600041 PM 8570628 ER PT J AU Turgeon, SM Pollack, AE Schusheim, L Fink, JS AF Turgeon, SM Pollack, AE Schusheim, L Fink, JS TI Effects of selective adenosine A(1) and A(2a) agonists on amphetamine-induced locomotion and c-Fos in striatum and nucleus accumbens SO BRAIN RESEARCH LA English DT Article DE APEC; adenosine; amphetamine; c-Fos; CHA; nucleus accumbens; striatum ID MOLECULAR-CLONING; RAT NEOSTRIATUM; GENE-EXPRESSION; RECEPTORS; LOCALIZATION; BEHAVIOR; NEURONS; STIMULATION; FOREBRAIN; BRAIN AB Low to moderate doses of amphetamine produce locomotion which is dependent on release of dopamine in the anteromedial striatum and nucleus accumbens. The effects of selective adenosine A(1) and A(2a) receptor agonists on locomotion and c-Fos induction following a moderate dose of amphetamine was assessed in rats. Pretreatment with the adenosine A(1) receptor agonist N-6-cyclohexyladenosine (CHA) or the adenosine A(2a) receptor agonist 2-[(2-aminoethylamino)carbonylethylphenylethylamino]-5'-N-ethylcarboxamidoadenosine (APEC) inhibited locomotion following an injection of amphetamine (1.5 mg/kg). This dose of amphetamine induced Fos-like immunoreactivity in an antero-dorsomedial distribution in the caudate-putamen and uniformly in the core and shell of the nucleus accumbens. Pretreatment with the adenosine A(2a) receptor agonist APEC, but not the adenosine A(1) receptor agonist CHA, attenuated c-Fos induction in caudate-putamen and nucleus accumbens by amphetamine. These findings indicate that amphetamine-induced behavior is subject to modulation by adenosine receptors through mechanisms which are both related to and independent of c-Fos induction. C1 MASSACHUSETTS GEN HOSP,MOLEC NEUROBIOL LAB,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT NEUROL,BOSTON,MA 02114. FU NIDA NIH HHS [DA07696]; NINDS NIH HHS [NS31579] NR 29 TC 34 Z9 34 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 22 PY 1996 VL 707 IS 1 BP 75 EP 80 DI 10.1016/0006-8993(95)01223-0 PG 6 WC Neurosciences SC Neurosciences & Neurology GA TU737 UT WOS:A1996TU73700008 PM 8866715 ER PT J AU Rock, K AF Rock, K TI Hot papers - Immunology - Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules by K.L Rock, C. Gramm, L. Rothstein, K. Clark, R. Stein, L. Dick, D. Hwang, A.L. Goldberg - Comments SO SCIENTIST LA English DT Editorial Material AB Hot Papers: Immunologist Kenneth Rock discusses how major histocompatibility complex-associated peptides are generated. C1 HARVARD UNIV,SCH MED,CAMBRIDGE,MA 02138. RP Rock, K (reprint author), DANA FARBER CANC INST,DIV LYMPHOCYTE BIOL,BOSTON,MA 02115, USA. NR 4 TC 0 Z9 0 U1 2 U2 6 PU SCIENTIST INC PI PHILADELPHIA PA 3600 MARKET ST SUITE 450, PHILADELPHIA, PA 19104 SN 0890-3670 J9 SCIENTIST JI Scientist PD JAN 22 PY 1996 VL 10 IS 2 BP 15 EP 15 PG 1 WC Information Science & Library Science; Multidisciplinary Sciences SC Information Science & Library Science; Science & Technology - Other Topics GA TQ903 UT WOS:A1996TQ90300012 ER PT J AU Wesselborg, S Fruman, DA Sagoo, JK Bierer, BE Burakoff, SJ AF Wesselborg, S Fruman, DA Sagoo, JK Bierer, BE Burakoff, SJ TI Identification of a physical interaction between calcineurin and nuclear factor of activated T cells (NFATp) SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CYCLOSPORINE-A; INTERLEUKIN-4 GENE; BINDING-PROTEIN; TRANSCRIPTION; EXPRESSION; PROMOTER; CYCLOPHILIN; AFFINITY; ELEMENTS; TARGET AB In T lymphocytes, the calcium/calmodulin-dependent serine/threonine phosphatase, calcineurin, plays a pivotal role in transducing membrane-associated signals to the nucleus. One of the putative targets of calcineurin is the pre-existing, cytosolic component of the nuclear factor of activated T cells (NFATp; also referred to as NFAT1), which is one of several transcription factors required for the expression of interleukin 2. Inhibition of calcineurin by the immunosuppressive drugs cyclo-sporin A and FK506 prevents dephosphorylation of NFATp and its translocation to the nucleus. However, a physical interaction between calcineurin and NFATp has not been demonstrated. Here we demonstrate the binding of NFATp from lysates of T cells to immobilized calcineurin. Stimulation of T cells with calcium ionophore induced a shift in the molecular weight of NFATp that is due to its dephosphorylation. This dephosphorylation was inhibited by treatment of T cells with cyclo-sporin A or FK506 prior to stimulation. Of note, both the phosphorylated and the dephosphorylated form of NFATp bound to calcineurin. Furthermore, the binding of both forms of NFATp to calcineurin was inhibited by pretreatment of calcineurin with a complex of FK506 and its ligand FKBP12. Taken together these data strongly suggest a direct interaction of calcineurin with NFATp and that this interaction does not depend upon the phosphorylation sites of NFATp affected by activation. C1 DANA FARBER CANC INST,DIV PEDIAT ONCOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. FU NIAID NIH HHS [AI 32514] NR 32 TC 75 Z9 75 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 19 PY 1996 VL 271 IS 3 BP 1274 EP 1277 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TQ525 UT WOS:A1996TQ52500007 PM 8576111 ER PT J AU Neumeyer, JL Tamagnan, G Wang, SY Gao, YG Milius, RA Kula, NS Baldessarini, RJ AF Neumeyer, JL Tamagnan, G Wang, SY Gao, YG Milius, RA Kula, NS Baldessarini, RJ TI N-substituted analogs of 2 beta-carbomethoxy-3 beta-(4'-iodophenyl)tropane (beta-CIT) with selective affinity to dopamine or serotonin transporters in rat forebrain SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID MONOAMINE REUPTAKE SITES; NONHUMAN-PRIMATES; COCAINE BINDING; H-3 COCAINE; PARKINSONS-DISEASE; BRAIN; INHIBITION; RECEPTORS; MEMBRANES; STRIATUM AB This report concerns the synthesis and chemical characterization of novel series of N-substituted 2 beta-carbomethoxy-3 beta-(4'-iodophenyl)tropan (beta-CIT, 2) analogs and their neuropharmacological evaluation for affinity at dopamine (DA(T)), serotonin (5-HTT), and norepinephrine membrane transporters in rat brain tissue. N-Substituted analogs of beta-CIT with a 2 beta-carbomethoxy ester moiety showed lower DAT affinity than beta-CIT for the DA(T), and some were more selective for the 5-HTT over the DA(T). 2 beta-Carbomethoxy(iodophenyl)nortropane analogs of beta-CIT with the N-substituents difluoroethyl, mesoxypropyl, iodopropyl, and methylpropionyl all yielded >10-fold lower DA(T) affinity than beta-CIT itself, whereas the N-(fluoropropyl)-2 beta-isopropyl ester analog (1) of beta-CIT exceeded beta-CIT (2, an N-methyl-2 beta-carbomethoxy ester) in DA(T) affinity. Several N-haloalkyl-substituted beta-CIT analogs yielded high 5-HTT affinity (K-i < 0.6 nM), ranking: N-fluoropropyl (5) > N-chloropropyl (4) greater than or equal to N-bromopropyl (3) > beta-CIT (2) > N-3'-phthalimidopropyl (11), with particularly high (ca. 30-fold) 5-HTT-over-DA(T) selectivity found in the N-fluoropropyl (5) and N-fluoroethyl (6) compounds, compared to only 3.0-fold 5-HTT selectivity in beta-CIT itself. Highly 5-HTT selective agents such as 5 and 6 may be useful as brain-imaging ligands for serotonin neurons or as mood-elevating drugs, while the high affinity and selectivity for the DA transporter found in N-(fluoropropyl)-2 beta-(carboxyisopropyl)-3 beta nortropane (1) and N-(fluoropropyl)-2 beta-carboxymethoxy-3 beta-(4'-iodophenyl)-nortropane (FP-beta-CIT; 5) support their use as improved markers for DA neurons. C1 HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,MCLEAN DIV,DEPT PSYCHIAT & NEUROSCI PROGRAM,BELMONT,MA 02178. RP Neumeyer, JL (reprint author), RES BIOCHEM INT,1 STRATHMORE RD,NATICK,MA 01760, USA. FU NIMH NIH HHS [MH-31154, MH-34006, MH-49522] NR 33 TC 76 Z9 81 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD JAN 19 PY 1996 VL 39 IS 2 BP 543 EP 548 DI 10.1021/jm9505324 PG 6 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA TR125 UT WOS:A1996TR12500027 PM 8558525 ER PT J AU Finger, EB Puri, KD Alon, R Lawrence, MB vonAndrian, UH Springer, TA AF Finger, EB Puri, KD Alon, R Lawrence, MB vonAndrian, UH Springer, TA TI Adhesion through L-selectin requires a threshold hydrodynamic shear SO NATURE LA English DT Article ID NODE HOMING RECEPTOR; HUMAN NEUTROPHILS; LECAM-1; IDENTIFICATION; LEUKOCYTES; SURFACE; GMP-140; MAC-1; RATES; FLOW AB SELECTINS are cell adhesion molecules that bind carbohydrate ligands and promote interaction between leukocytes and the vessel wall in vascular shear flow(1,2). Selectin-ligand bonds have high mechanical strength, allowing initial tethering to the vessel wall through one or few bonds, and have fast on and off rates, permitting rolling in response to hydrodynamic drag(3). The L-selectin molecule on leukocytes binds to peripheral node addressin on high endothelial venules of lymph nodes to mediate leukocyte rolling(4,5) and binds to a ligand on neutrophils to mediate rolling of leukocytes over one another(6). Here we describe a surprising mechanism for regulation of these interactions, both in vitro and in vivo. Shear above a critical threshold is required to promote and maintain rolling interactions through L-selectin, but not through E-selectin, P-selectin or VCAM-1. The shear threshold requirement for L-selectin may be physiologically important in low shear to prevent inappropriate aggregation of leukocytes and interaction with the vessel wall. C1 HARVARD UNIV,SCH MED,CTR BLOOD RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. UNIV VIRGINIA,DEPT BIOMED ENGN,CHARLOTTESVILLE,VA 22903. RI von Andrian, Ulrich/A-5775-2008 NR 29 TC 357 Z9 362 U1 1 U2 13 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD JAN 18 PY 1996 VL 379 IS 6562 BP 266 EP 269 DI 10.1038/379266a0 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TQ169 UT WOS:A1996TQ16900049 PM 8538793 ER PT J AU Eck, MJ Pluskey, S Trub, T Harrison, SC Shoelson, SE AF Eck, MJ Pluskey, S Trub, T Harrison, SC Shoelson, SE TI Spatial constraints on the recognition of phosphoproteins by the tandem SH2 domains of the phosphatase SH-PTP2 SO NATURE LA English DT Article ID SH2-CONTAINING PHOSPHOTYROSINE PHOSPHATASE; FACTOR RECEPTOR-BETA; TYROSINE PHOSPHORYLATION; PROTEIN MODELS; ASSOCIATION; ACTIVATION; SUBUNIT; SYP AB THE domain organization of many signalling proteins facilitates a segregation of binding, catalytic and regulatory functions(1,2). The mammalian SH2 domain protein tyrosine phosphatases (PTPs) contain tandem SH2 domains and a single carboxy-terminal catalytic domain(3). SH-PTP1 (PTP1C, HCP) and SH-PTP2 (Syp, PTP2C, PTP1D) function downstream from tyrosine kinase-linked insulin, growth factor, cytokine and antigen receptors(4-12). As well as directing subcellular localization by binding to receptors and their substrates, the two SH2 domains of these PTPs function together to regulate catalysis(7,13,14). Here we report the structure of the tandem SH2 domains of SH-PTP2 in complex with monophosphopeptides. A fixed relative orientation of the two domains, stabilized by a disulphide bond and a small hydrophobic patch within the interface, separates the peptide binding sites by similar to 40 Angstrom. The defined orientation of the SH2 domains in the structure, and data showing that peptide orientation and spacing between binding sites is critical for enzymatic activation, suggest that spatial constraints are important in this multidomain protein-protein interaction. C1 HARVARD UNIV,CHILDRENS HOSP,MOLEC MED LAB,BOSTON,MA 02115. HARVARD UNIV,SCH MED,JOSLIN DIABET CTR,DIV RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. RP Eck, MJ (reprint author), HARVARD UNIV,CHILDRENS HOSP,SCH MED,HOWARD HUGHES MED INST,BOSTON,MA 02115, USA. NR 30 TC 147 Z9 150 U1 0 U2 4 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD JAN 18 PY 1996 VL 379 IS 6562 BP 277 EP 280 DI 10.1038/379277a0 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TQ169 UT WOS:A1996TQ16900052 PM 8538796 ER PT J AU FitzGerald, MG MacDonald, DJ Krainer, M Hoover, I ONeil, E Unsal, H SilvaArrieto, S Finkelstein, DM BeerRomero, P Englert, C Sgroi, DC Smith, BL Younger, JW Garber, JE Duda, RB Mayzel, KA Isselbacher, KJ Friend, SH Haber, DA AF FitzGerald, MG MacDonald, DJ Krainer, M Hoover, I ONeil, E Unsal, H SilvaArrieto, S Finkelstein, DM BeerRomero, P Englert, C Sgroi, DC Smith, BL Younger, JW Garber, JE Duda, RB Mayzel, KA Isselbacher, KJ Friend, SH Haber, DA TI Germ-line BRCA1 mutations in Jewish and non-Jewish women with early-onset breast cancer SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID MEDICAL PROGRESS; FAMILIAL BREAST; LINKAGE; HISTORY; RISK AB Background. Mutations in a germ-line allele of the BRCA1 gene contribute to the familial breast cancer syndrome. However, the prevalence of these mutations is unknown in women with breast cancer who do not have the features of this familial syndrome. We sought BRCA1 mutations in women who were given a diagnosis of breast cancer at an early age, because early onset is characteristic of a genetic predisposition to cancer. Methods. Clinical information and peripheral-blood mononuclear cells were obtained from 418 women from the Boston metropolitan area in whom breast cancer was diagnosed at or before the age of 40. A comprehensive BRCA1 mutational analysis, involving automated nucleotide sequencing and a protein-truncation assay, was undertaken in 30 of these women, who had breast cancer before the age of 30. In addition, the BRCA1 mutation 185delAG, which is prevalent in the Ashkenazi Jewish population, was sought with an allele-specific polymerase-chain-reaction assay in 39 Jewish women among the 418 women who had breast cancer at or before the age of 40. Results. Among 30 women with breast cancer before the age of 30, 4 (13 percent) had definite, chain-terminating mutations and 1 had a missense mutation. Two of the four Jewish women in this cohort had the 185delAG mutation, Among the 39 Jewish women with breast cancer at or before the age of 40, 8 (21 percent) carried the 185delAG mutation (95 percent confidence interval, 9 to 36 percent). Conclusions. Germ-line BRCA1 mutations can be present in young women with breast cancer who do not belong to families with multiple affected members. The specific BRCA1 mutation known as 185delAG is strongly associated with the onset of breast cancer in Jewish women before the age of 40. C1 MASSACHUSETTS GEN HOSP,CTR CANC,BOSTON,MA 02129. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT MED & SURG ONCOL,BOSTON,MA 02114. HARVARD UNIV,SCH PUBL HLTH,DEPT BIOSTAT,BOSTON,MA 02115. DANA FARBER CANC INST,BOSTON,MA 02115. BETH ISRAEL HOSP,BOSTON,MA 02215. FAULKNER HOSP,BOSTON,MA 02130. CTR CANC RISK ANAL,BOSTON,MA. NR 27 TC 310 Z9 314 U1 0 U2 3 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 18 PY 1996 VL 334 IS 3 BP 143 EP 149 DI 10.1056/NEJM199601183340302 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA TQ017 UT WOS:A1996TQ01700002 PM 8531968 ER PT J AU Cheng, TJ Christiani, DC Xu, XP Wain, JC Wiencke, JK Kelsey, KT AF Cheng, TJ Christiani, DC Xu, XP Wain, JC Wiencke, JK Kelsey, KT TI Increased micronucleus frequency in lymphocytes from smokers with lung cancer SO MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS LA English DT Article DE micronucleus; lung cancer; T-lymphocyte ID MUTAGEN SENSITIVITY; SUSCEPTIBILITY; SUBSETS; DAMAGE; GSTM1; RISK AB We investigated whether lung cancer was associated with an increased micronucleus (MN) frequency in lymphocytes in a case-control study. Epidemiological data were obtained by an interviewer-administered questionnaire and included information on smoking history, intake of dietary micronutrients, general medical history, environmental and occupational exposures to mutagens and carcinogens, and family history of cancer. A modified cytokinesis-block method was used to determine individual MN frequency. Polymorphisms in glutathione S-transferase class mu were determined by PCR analysis. Overall, 55 controls and 42 cases were studied. MN frequency in cases and controls was not associated with age, smoking, metabolic genetic polymorphisms, environmental and occupational exposures, or medical history. Female controls had a significantly higher MN frequency than male controls (p = 0.05). Overall, MN frequency was significantly higher in cases than in controls (p < 0.01). Twenty-four cases (57%) had an MN frequency higher than the upper 95% confidence interval of the mean value for controls (11.5 MNs/1000 binucleated cells). Further analysis showed that, cases who were current and former smokers had significantly higher MN frequencies than controls (p = 0.04); this difference was not seen in the group that had never smoked. The significantly higher MN frequency among cases with a history of smoking may be attributable to the presence of lung neoplasm per se or to the interaction of smoking with endogenous factors associated with the development of lung cancer. C1 HARVARD UNIV,SCH PUBL HLTH,DEPT CANC BIOL,BOSTON,MA 02115. HARVARD UNIV,SCH PUBL HLTH,DEPT ENVIRONM HLTH,OCCUPAT HLTH PROGRAM,BOSTON,MA 02115. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,PULM & CRIT CARE UNIT,BOSTON,MA 02114. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,THORAC SURG UNIT,BOSTON,MA 02114. UNIV CALIF SAN FRANCISCO,DEPT BIOSTAT & EPIDEMIOL,LAB MOLEC EPIDEMIOL,SAN FRANCISCO,CA 94143. RI Cheng, Tsun-Jen /D-3495-2012; Kelsey, Karl/I-1252-2014; OI CHENG, TSUN-JEN/0000-0002-2613-8230 FU NIEHS NIH HHS [ES-00002, ES/CA-06409] NR 28 TC 26 Z9 26 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0027-5107 J9 MUTAT RES-FUND MOL M JI Mutat. Res.-Fundam. Mol. Mech. Mutagen. PD JAN 17 PY 1996 VL 349 IS 1 BP 43 EP 50 DI 10.1016/0027-5107(95)00150-6 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology SC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology GA TR095 UT WOS:A1996TR09500004 PM 8569791 ER PT J AU Zaim, BR Zaim, SH Rankin, AC McGovern, BA Garan, H Ruskin, JN AF Zaim, BR Zaim, SH Rankin, AC McGovern, BA Garan, H Ruskin, JN TI Comparison of cycle lengths between induced and spontaneous sustained ventricular tachycardia during concordant antiarrhythmic therapy associated with healed myocardial infarction SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID IMPLANTABLE CARDIOVERTER-DEFIBRILLATOR; CLINICAL-EXPERIENCE RP Zaim, BR (reprint author), MASSACHUSETTS GEN HOSP,CARDIAC UNIT,BOSTON,MA 02114, USA. NR 8 TC 0 Z9 0 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD JAN 15 PY 1996 VL 77 IS 2 BP 202 EP 204 DI 10.1016/S0002-9149(96)90601-3 PG 3 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA TQ963 UT WOS:A1996TQ96300023 PM 8546096 ER PT J AU Leon, SP Folkerth, RD Black, PM AF Leon, SP Folkerth, RD Black, PM TI Microvessel density is a prognostic indicator for patients with astroglial brain tumors SO CANCER LA English DT Article DE microvessel counts; angiogenesis; brain neoplasm; glioma; prognosis; immunohistochemistry; factor VIII-related antigen; univariate analysis; multivariate analysis ID FIBROBLAST GROWTH-FACTOR; INVASIVE BREAST-CARCINOMA; MALIGNANT GLIOMA; GLIOBLASTOMA-MULTIFORME; RADIATION-THERAPY; ANAPLASTIC GLIOMAS; REGRESSION-MODELS; LABELING INDEX; ANGIOGENESIS; CANCER AB BACKGROUND, Microvessel density in tumors, a measure of angiogenesis, has been shown to be a prognostic indicator that correlates with an increased risk of metastasis in various epithelial cancers and with overall and relapse free survival in patients with breast cancer. Astrocytic brain tumors, particularly malignant astrocytomas, are recognized to be highly vascular tumors with potent angiogenic activity. However, the prognostic significance of microvessel density in these tumors is not known. METHODS, Sections from formalin fixed paraffin embedded tumor tissue from 93 unselected adult patients with supratentorial astrocytic brain tumors were immunostained for factor VIII-related antigen in order to highlight microvessel endothelial cells. Microvessels were counted at 200x and 400x magnification. Microvessel density was graded as 1+ to 4+ on 1 low power field, without knowledge of clinical outcome. Microvessel count and microvessel grade were correlated with postoperative survival using the Cox proportional hazards regression model. The prognostic significance of microvessel count and grade were also compared with established prognostic indicators, including patient age, Karnofsky performance status, and tumor histology using multivariate analyses. RESULTS, Both microvessel grade and microvessel count correlated significantly with postoperative survival by univariate analysis in both previously untreated and treated patients. Patients with tumors containing a microvessel Grade of 3+ or 4+ had significantly shorter survival time than patients with a microvessel Grade of 1+ or 2+ (P = 0.0022). Likewise, patients with microvessel counts of 70 or greater had significantly shorter survival than those with microvessel counts of fewer than 70 (P = 0.041). Patient age, Karnofsky performance status, tumor histology, and extent of resection were also correlated with survival by univariate analysis. Microvessel count was further shown to be an independent prognostic indicator by multivariate analyses. There were correlations between microvessel density and patient age and between microvessel density and astrocytic tumor grade. CONCLUSIONS, These findings support the importance of microvessel density as a prognostic indicator of postoperative survival of patients with astroglial brain tumors. Regional tumor heterogeneity may limit the use of these techniques for routine pathologic examination. (C) 1996 American Cancer Society. C1 HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,CHILDRENS HOSP,DANA FARBER CANC INST,NEUROSURG LABS,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BRIGHAM & WOMENS HOSP,CHILDRENS HOSP,DANA FARBER CANC INST,BRAIN TUMOR CTR,BOSTON,MA 02115. HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT PATHOL,DIV NEUROPATHOL,BOSTON,MA 02115. FU NINDS NIH HHS [NS31110] NR 63 TC 336 Z9 367 U1 0 U2 9 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD JAN 15 PY 1996 VL 77 IS 2 BP 362 EP 372 DI 10.1002/(SICI)1097-0142(19960115)77:2<362::AID-CNCR20>3.0.CO;2-Z PG 11 WC Oncology SC Oncology GA TR256 UT WOS:A1996TR25600020 PM 8625246 ER PT J AU Price, BD Youmell, MB AF Price, BD Youmell, MB TI The phosphatidylinositol 3-kinase inhibitor wortmannin sensitizes murine fibroblasts and human tumor cells to radiation and blocks induction of p53 following DNA damage SO CANCER RESEARCH LA English DT Article ID ATAXIA-TELANGIECTASIA; BINDING; KINASE; CHECKPOINT AB AT cells are extremely sensitive to ionizing radiation, Since the AT gene has homology to phosphatidylinositol 3 kinases (PI 3-kinases), wortmannin, a specific inhibitor of PI 3-kinase, was used to determine if PI 3-kinase activity regulates radiation sensitivity. Human and murine cells exposed to wortmannin alone did not display significant cytotoxicity. Wortmannin in combination with radiation was an effective radiosensitizer of murine NiH-3T3 fibroblasts, with a sensitizer enhancement ratio of 1.8 at 10% survival, and had a similar effect on the human tumor cell lines HeLa, SW480, and MCF-7. Wortmannin inhibited the induction of p53 DNA-binding activity by actinomycin D and radiation and blocked the transcriptional activation of a p53 CAT reporter gene by actinomycin D. Wortmannin radiosensitized both wild-type (NIH-3T3 and MCF-7) and mutant (SW480 and HeLa) p53 cells, indicating that p53 induction was not required for radiosensitization by wortmannin. The results suggest that a wortmannin-sensitive pathway, possibly involving PI 3-kinase activity, may regulate the response of the cells to DNA damage. RP Price, BD (reprint author), DANA FARBER CANC INST,JOINT CTR RADIAT THERAPY,JF209,44 BINNEY ST,BOSTON,MA 02115, USA. FU NCI NIH HHS [CA64585] NR 21 TC 136 Z9 140 U1 0 U2 2 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 15 PY 1996 VL 56 IS 2 BP 246 EP 250 PG 5 WC Oncology SC Oncology GA TP888 UT WOS:A1996TP88800004 PM 8542574 ER PT J AU Clement, MV Stamenkovic, I AF Clement, MV Stamenkovic, I TI Superoxide anion is a natural inhibitor of Fas-mediated cell death SO EMBO JOURNAL LA English DT Article DE apoptosis; Fas; superoxide anion; tumor cells ID PROTEIN-KINASE-C; TUMOR NECROSIS FACTOR; GENERATING OXIDASE; NADPH OXIDASE; NITRIC-OXIDE; GENE CED-3; NEUTROPHILS; DISMUTASE; ACTIVATION; APOPTOSIS AB The cell surface receptor Fas is a major trigger of apoptosis. However, expression of the Fas receptor in many tumor cell types does not correlate with sensitivity to Fas-mediated cell death, Because a prooxidant state is a common feature of tumor cells, we examined the role of intracellular reactive oxygen intermediates in the regulation of Fas-mediated cytotoxicity. Our results show that an oxidative stress induced by increasing the intracellular superoxide anion (O-2(-)) concentration can abrogate Fas-mediated apoptosis In cells which are constitutively sensitive to Fas, Conversely, an O-2(-) concentration decrease is observed to sensitize cells which are naturally resistant to Fas signals, These observations suggest that intracellular O-2(-) may play a key role in regulating cell sensitivity to a potentially lethal signal and provide tumor cells with a natural, inducible mechanism of resistance to Fas-mediated apoptosis. C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02129. RP Clement, MV (reprint author), HARVARD UNIV,SCH MED,DEPT PATHOL,149 13TH ST,CHARLESTOWN NAVY YARD,BOSTON,MA 02129, USA. FU NCI NIH HHS [CA55735] NR 63 TC 215 Z9 215 U1 1 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD JAN 15 PY 1996 VL 15 IS 2 BP 216 EP 225 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA TR572 UT WOS:A1996TR57200003 PM 8617197 ER PT J AU Beard, CJ Kijewski, P Bussiere, M Gelman, R Gladstone, D Shaffer, K Plunkett, M Costello, P Coleman, CN AF Beard, CJ Kijewski, P Bussiere, M Gelman, R Gladstone, D Shaffer, K Plunkett, M Costello, P Coleman, CN TI Analysis of prostate and seminal vesicle motion: Implications for treatment planning SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article DE prostate neoplasm; treatment planning; conformal therapy; radiation therapy ID RADIOTHERAPY AB Purpose: To quantify prostate and seminal vesicle positional changes (target motion) between treatment planning and delivery, and to identify the factors contributing to target motion. Methods and Materials: Thirty patients with adenocarcinoma of the prostate were prospectively evaluated by analyzing two sequential planning computerized tomography (CT) scans (S-1, obtained prior to treatment, and S-2 obtained during the fourth week of treatment) for each patient. All anatomical volumes of interest (soft tissue and bony) were reconstructed from transverse CT images and projected onto anterior and lateral beam's-eye view projections. Positional changes between S-1 and S-2 were eliminated by applying a rigid body translation and rotation. Target motion was then measured by recording the positional change between S-1 and S-2 at the edges (right, left, superior, inferior). Potential correlation of target motion with bladder volume, rectal volume, and rectal diameter changes were evaluated by linear regression analysis. Results: Neither the prostate nor seminal vesicles remained fixed with respect to bony anatomy between S-1 and S-2. The distribution of positional changes were generally small (< 0.5 cm), but maximum displacements of 1.5-2.2 cm did occur, particularly in the lateral view. In this study, bladder volume changes between the scans were small and did not correlate with target motion (p = 0.67). Both rectal volume and rectal diameter changes correlated with target motion for both the prostate (p = 0.004 and 0.005, respectively) and seminal vesicles (p < 0.001 and < 0.001, respectively). However, neither the initial rectal volume nor the initial rectal diameter could be used to predict subsequent target motion when evaluated either singly or as part of a multiple regression model. Conclusions: Target motion occurs during the course of treatment planning and delivery and should be considered when designing conformal radiation fields. Although the target position at the time of planning CT may differ substantially from the mean treatment position, target motion cannot be predicted by evaluating simply measured parameters from a single scan, or double scan sequence. C1 HARVARD UNIV,NEW ENGLAND DEACONESS HOSP,SCH MED,DANA FARBER CANC INST,DEPT RADIOL,BOSTON,MA 02115. HARVARD UNIV,NEW ENGLAND DEACONESS HOSP,SCH MED,DEPT RADIOL,BOSTON,MA 02115. RP Beard, CJ (reprint author), HARVARD UNIV,NEW ENGLAND DEACONESS HOSP,SCH MED,JOINT CTR RADIAT THERAPY,50 BINNEY ST,BOSTON,MA 02115, USA. NR 8 TC 176 Z9 179 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0360-3016 J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD JAN 15 PY 1996 VL 34 IS 2 BP 451 EP 458 DI 10.1016/0360-3016(95)02081-0 PG 8 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA TR104 UT WOS:A1996TR10400023 PM 8567348 ER PT J AU Pinsky, DJ Naka, Y Liao, H Oz, MC Wagner, DD Mayadas, TN Johnson, RC Hynes, RO Heath, M Lawson, CA Stern, DM AF Pinsky, DJ Naka, Y Liao, H Oz, MC Wagner, DD Mayadas, TN Johnson, RC Hynes, RO Heath, M Lawson, CA Stern, DM TI Hypoxia-induced exocytosis of endothelial cell Weibel-Palade bodies mechanism for rapid neutrophil recruitment after cardiac preservation SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE ischemia; Weibel-Palade body; neutrophils; P-selectin; von Willebrand factor ID GRANULE MEMBRANE-PROTEIN; P-SELECTIN; VONWILLEBRAND-FACTOR; MYOCARDIAL-ISCHEMIA; ACTIVATED PLATELETS; REPERFUSION INJURY; MONOLAYER PERMEABILITY; MULTIMERIC COMPOSITION; STIMULATED SECRETION; MONOCLONAL-ANTIBODY AB The period of hypoxia is an important priming event for the vascular dysfunction that accompanies reperfusion, with endothelial cells (ECs) and neutrophils (PMNs) playing a central role. We hypothesized that EC Weibel-Palade (WP) body exocytosis during the hypoxic/ischemic period during organ preservation permits brisk PMN recruitment into postischemic tissue, a process further amplified in an oxidant-rich milieu. Exposure of human umbilical vein ECs to a hypoxic environment (pO(2) approximate to 20 torr) stimulated release of von Willebrand factor (vWF), stored in EC WP bodies, as well as increased expression of the WP body-derived PMN adhesion molecule P-selectin at the EC surface. Increased binding of In-111-labeled PMNs to hypoxic EC monolayers (compared with normoxic controls) was blocked with a blocking antibody to P-selectin, but was not affected by a nonblocking control antibody. Although increased P-selectin expression and vWF release were also noted during reoxygenation, hypoxia alone (even in the presence of antioxidants) was sufficient to increase WP body exocytosis. To determine the relevance of these observations to hypothermic cardiac preservation, during which the pO(2) within the cardiac vasculature declines to similarly low levels, experiments were performed in a rodent (rat and mouse) cardiac preservation/transplantation model. Immunodepletion of recipient PMNs or administration of a blocking anti-P-selectin antibody before transplantation resulted in reduced graft neutrophil infiltration and improved graft survival, compared with identically preserved hearts transplanted into control recipients. To establish the important role of endothelial P-selectin expression on the donor vasculature, murine cardiac transplants were performed using homezygous P-selectin deficient and wild-type control donor hearts flushed free of blood/platelets before preservation/transplantation. P-selectin-null hearts transplanted into wild-type recipients demonstrated a marked (13-fold) reduction in graft neutrophil infiltration and increased graft survival compared with wild-type hearts transplanted into wild-type recipients. To determine whether coronary endothelial WP exocytosis may occur during cardiac preservation in humans, the release of vWF into the coronary sinus (CS) was measured in 32 patients during open heart surgery. CS samples obtained at the start and conclusion of the ischemic period demonstrated an increase in CS vWF antigen (by ELISA) consisting of predominantly high molecular weight multimers (by immunoelectrophoresis). These data suggest that EC WP exocytosis occurs during hypothermic cardiac preservation, priming the vasculature to recruit PMNs rapidly during reperfusion. C1 COLUMBIA UNIV COLL PHYS & SURG,DEPT SURG,NEW YORK,NY 10032. COLUMBIA UNIV COLL PHYS & SURG,DEPT ANESTHESIOL,NEW YORK,NY 10032. COLUMBIA UNIV COLL PHYS & SURG,DEPT PHYSIOL,NEW YORK,NY 10032. HARVARD UNIV,SCH MED,CTR BLOOD RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. MIT,CTR CANC RES,HOWARD HUGHES MED INST,DEPT BIOL,CAMBRIDGE,MA 02139. RP Pinsky, DJ (reprint author), COLUMBIA UNIV COLL PHYS & SURG,DEPT MED,P&S 11-518,630 W 168 ST,NEW YORK,NY 10032, USA. FU NHLBI NIH HHS [HL41002, HL41484, HL50629] NR 56 TC 187 Z9 192 U1 1 U2 4 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JAN 15 PY 1996 VL 97 IS 2 BP 493 EP 500 DI 10.1172/JCI118440 PG 8 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA TR380 UT WOS:A1996TR38000029 PM 8567972 ER PT J AU StLezin, EM Pravenec, M Wong, AL Liu, WH Wang, N Lu, SH Jacob, HJ Roman, RJ Stec, DE Wang, JM Reid, IA Kurtz, TW AF StLezin, EM Pravenec, M Wong, AL Liu, WH Wang, N Lu, SH Jacob, HJ Roman, RJ Stec, DE Wang, JM Reid, IA Kurtz, TW TI Effects of renin gene transfer on blood pressure and renin gene expression in congenic strain of dahl salt-resistant rats SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE rat; inbred Dahl salt sensitive; genetics; hypertension; renin angiotensin system ID SPONTANEOUSLY HYPERTENSIVE RAT; ANGIOTENSIN SYSTEM; BREEDING PROGRAMS; DNA; MARKERS; LOCI; COSEGREGATION; INTROGRESSION; CHROMOSOME-13; REGIONS AB To investigate whether a BP-regulatory locus exists in the vicinity of the renin locus on rat chromosome 13, we transferred this chromosome segment from the Dahl salt-sensitive (S) rat onto the genetic background of the Dahl salt-resistant (R) rat. In congenic Dahl R rats carrying the S renin gene and fed an 8% salt diet, systolic BP was significantly lower than in progenitor Dahl R rats: 127+/-1 mmHg versus 138+/-4 mmHg, respectively (P < 0.05). Moreover, the decreased BP in the congenic Dahl R strain was associated with decreased kidney renin mRNA and decreased plasma renin concentration. These findings demonstrate that the Dahl S strain carries alleles in or near the renin locus that confer lower plasma renin concentration and lower BP than the corresponding alleles in the Dahl R strain, at least when studied on the genetic background of the Dahl R rat and in the environment of a high salt diet. The occurrence of coincident reductions in kidney renin mRNA, plasma renin concentration, and BP after interstrain transfer of naturally occurring renin gene variants strongly suggests that genetically determined variation in renin gene expression can affect BP. C1 UNIV CALIF SAN FRANCISCO,DEPT LAB MED,SAN FRANCISCO,CA 94143. ACAD SCI CZECH REPUBL,INST PHYSIOL,CR-14220 PRAGUE 4,CZECH REPUBLIC. MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,CHARLESTON,MA 02129. MED COLL WISCONSIN,DEPT PHYSIOL,MILWAUKEE,WI 53226. UNIV CALIF SAN FRANCISCO,DEPT PHYSIOL,SAN FRANCISCO,CA 94143. RI Pravenec, Michal/B-1666-2012 FU NHLBI NIH HHS [HL-35018] NR 40 TC 44 Z9 44 U1 0 U2 2 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JAN 15 PY 1996 VL 97 IS 2 BP 522 EP 527 DI 10.1172/JCI118444 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA TR380 UT WOS:A1996TR38000033 PM 8567976 ER PT J AU Molnar, A Wu, P Largespada, DA Vortkamp, A Scherer, S Copeland, NG Jenkins, NA Bruns, G Georgopoulos, K AF Molnar, A Wu, P Largespada, DA Vortkamp, A Scherer, S Copeland, NG Jenkins, NA Bruns, G Georgopoulos, K TI The Ikaros gene encodes a family of lymphocyte-restricted zinc finger DNA binding proteins, highly conserved in human and mouse SO JOURNAL OF IMMUNOLOGY LA English DT Article ID RECEPTOR-ALPHA-GENE; T-CELL DEVELOPMENT; FUNCTIONAL-CHARACTERIZATION; TRANSCRIPTION FACTORS; KAPPA-B; EXPRESSION; IDENTIFICATION; REGULATOR; SEQUENCES; MEDIATOR AB The Ikaros gene is an essential regulator in the development and homeostasis of the mouse lymphopoietic system, To study the role of the Ikaros gene in the human lymphopoietic system, we cloned and characterized human Ikaros cDNAs. In the human, as in the mouse, differential splicing of Ikaros primary transcripts generates a family of lymphoid-restricted zinc finger DNA binding proteins, highly conserved in sequence composition and relative expression to the mouse homologues, Expression of Ikaros isoforms is highly restricted to the lymphopoietic system and is particularly enriched in maturing thymocytes, The Ikaros gene maps at a syntenic locus located on the short arm of human chromosome 7 and on mouse chromosome 11 next to the epidermal growth factor receptor (Egfr), The high degree of conservation of the Ikaros gene at the genetic and expression levels strongly suggests that it plays a fundamental role in the ontogeny of the lymphopoietic system across species. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CUTANEOUS BIOL RES CTR,BOSTON,MA 02129. HARVARD UNIV,MASSACHUSETTS GEN HOSP,CHILDRENS HOSP,SCH MED,BOSTON,MA 02129. NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702. HOSP SICK CHILDREN,DEPT GENET,TORONTO,ON M5G 1X8,CANADA. KLINIKUM PHILIPPS UNIV,MARBURG,GERMANY. RI Howe, Jennifer/I-9013-2012; Scherer, Stephen /B-3785-2013 OI Scherer, Stephen /0000-0002-8326-1999 FU NIAID NIH HHS [R01AI33062] NR 22 TC 107 Z9 113 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD JAN 15 PY 1996 VL 156 IS 2 BP 585 EP 592 PG 8 WC Immunology SC Immunology GA TP366 UT WOS:A1996TP36600022 PM 8543809 ER PT J AU Lee, LA Sergio, JJ Sykes, M AF Lee, LA Sergio, JJ Sykes, M TI Natural killer cells weakly resist engraftment of allogeneic, long-term, multilineage-repopulating hematopoietic stem cells SO TRANSPLANTATION LA English DT Article ID BONE-MARROW TRANSPLANTATION; VS-HOST DISEASE; GRAFT-REJECTION; PECULIAR IMMUNOBIOLOGY; MONOCLONAL-ANTIBODIES; NK CELLS; MOUSE; MICE; DEPLETION; INVIVO AB Natural killer (NK) cells effect hybrid resistance, in which parental hematopoietic cell grafts are rejected by F-1 recipients, NK cells can also resist engraftment of fully MHC-mismatched allogeneic marrow, However, studies of NK cell-mediated alloresistance have relied on short-term proliferation, colony, or survival assays; therefore, their results may not reflect effects of Mt cells on the engraftment of allogeneic pluripotent hematopoietic stem cells (PHSC), We have now addressed the role of NK cells in resisting engraftment of these most primitive hematopoietic cells, which provide long-term repopulation of multiple hematopoietic lineages, We took advantage of a nonmyeloablative conditioning regimen that permits allogeneic marrow engraftment and induction of mixed chimerism in mice to evaluate the effect of host NK cell depletion with mAb PK136 on long term competitive repopulating ability of allogeneic marrow, Mice were pretreated with depleting anti-CD4 and anti-CD8 mAbs, then received 3 Gy of whole body irradiation and 7 Gy of thymic irradiation prior to allogeneic bone marrow transplantation. Depending on the strain combination used, statistically significant increases in long-term allogeneic repopulation of both myeloid and lymphoid cell lineages were observed in recipients depleted of NK cells before bone marrow transplantation compared with controls, Depletion of host NK cells alone was sufficient to enhance donor PHSC engraftment, However, a statistically significant increase in allogeneic reconstitution in NK cell-depleted chimeras compared with control chimeras was not observed in every experiment, and differences were most readily apparent in a strain combination in which recipient NR cells have been shown to have high resistance to engraftment of donor short-term repopulating cells, Chronic (16 weeks) anti-NK1.1 treatment resulted in higher levels of donor-type repopulation than that in animals receiving only pretransplant NK cell depletion, Our studies demonstrate for the first time that host NK cells resist engraftment of allogeneic longterm repopulating PHSC, and provide a model for studying the elements that determine what is regarded as ''self'' and ''non-self'' by newly developing NK cells. C1 MASSACHUSETTS GEN HOSP,TRANSPLANTAT BIOL RES CTR,SURG SERV,BOSTON,MA 02129. HARVARD UNIV,SCH MED,BOSTON,MA 02129. FU NCI NIH HHS [T32-CA09216-14]; NHLBI NIH HHS [R01 HL49915] NR 46 TC 66 Z9 66 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD JAN 15 PY 1996 VL 61 IS 1 BP 125 EP 132 DI 10.1097/00007890-199601150-00024 PG 8 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA TQ201 UT WOS:A1996TQ20100024 PM 8560550 ER PT J AU Zhu, JH Bilan, PJ Moyers, JS Antonetti, DA Kahn, CR AF Zhu, JH Bilan, PJ Moyers, JS Antonetti, DA Kahn, CR TI Rad, a novel ras-related GTPase, interacts with skeletal muscle beta-tropomyosin SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID BINDING PROTEIN; RAN/TC4 AB Rad, a prototypic member of a subfamily of Ras-related GTPases, is overexpressed in skeletal muscle of type II diabetic humans. By expression screening of mouse embryo and human skeletal muscle cDNA libraries, we found that Rad interacted with skeletal muscle beta-tropomyosin. In the mouse skeletal muscle cell line C2C12, this interaction was significantly increased by the calcium ionophore A23187. A23187 also caused a time and concentration-dependent decrease in total cellular Rad with increased interaction between tropomyosin and Rad in the detergent-soluble fraction and the appearance of Rad in the cytoskeleton. In C2C12 cells stably overexpressing a putative dominant negative mutant of Rad (S105N), there was an increase in the amount of tropomyosin in Rad immunoprecipitates. In cells overexpressing wild type Rad, much of Rad was associated with the cytoskeleton and was no longer responsive to A23187. In far-Western blotting and guanine nucleotide saturation studies, GDP-Rad bound to tropomyosin far better than GTP-Rad. We conclude that Rad interacts with skeletal muscle beta-tropomyosin and the cytoskeleton in a guanine nucleotide-dependent manner. These data suggest that Rad may be involved in skeletal muscle motor function and cytoskeletal organization. C1 JOSLIN DIABET CTR,DIV RES,BOSTON,MA 02215. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02215. FU NIDDK NIH HHS [DK 45935, DK 07260] NR 22 TC 46 Z9 46 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 12 PY 1996 VL 271 IS 2 BP 768 EP 773 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TP889 UT WOS:A1996TP88900028 PM 8557685 ER PT J AU Winawer, SJ Zauber, AG Gerdes, H OBrien, MJ Gottlieb, LS Sternberg, SS Bond, JH Waye, JD Schapiro, M Panish, JF Kurtz, RC Shike, M Ackroyd, FM Stewart, ET Skolnick, M Bishop, DT Lightdale, CJ Edelman, M Fleisher, M Ho, MN Diaz, B Lapidus, J Padern, RA Mandelman, M Nazario, H Colon, H Kadvan, P Miller, C Szporn, A Herpes, R Khilnani, M Ansel, H Ewing, S Dobson, T Hogan, W Helm, J Komorowski, R McLaughlin, E Greenen, J Venu, R Jonson, GK DeBoer, N Hedberg, S Shellito, P Hall, D Dickersin, G Horton, N Sherman, J Hamlin, JA Geller, S Kojimoto, M Auslander, M Kasimian, D Kussin, L Scoggins, C Magrath, C AF Winawer, SJ Zauber, AG Gerdes, H OBrien, MJ Gottlieb, LS Sternberg, SS Bond, JH Waye, JD Schapiro, M Panish, JF Kurtz, RC Shike, M Ackroyd, FM Stewart, ET Skolnick, M Bishop, DT Lightdale, CJ Edelman, M Fleisher, M Ho, MN Diaz, B Lapidus, J Padern, RA Mandelman, M Nazario, H Colon, H Kadvan, P Miller, C Szporn, A Herpes, R Khilnani, M Ansel, H Ewing, S Dobson, T Hogan, W Helm, J Komorowski, R McLaughlin, E Greenen, J Venu, R Jonson, GK DeBoer, N Hedberg, S Shellito, P Hall, D Dickersin, G Horton, N Sherman, J Hamlin, JA Geller, S Kojimoto, M Auslander, M Kasimian, D Kussin, L Scoggins, C Magrath, C TI Risk of colorectal cancer in the families of patients with adenomatous polyps SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID COLON-CANCER; NATIONAL POLYP; HISTORY; RECTUM; SURVEILLANCE; INHERITANCE; RELATIVES; MORTALITY; FREQUENCY; HOMOLOG AB Background. The adenoma-adenocarcinoma sequence in colorectal cancer suggests an increased risk of colorectal cancer in the families of patients with adenomatous polyps. Methods. A random sample of participants in the National Polyp Study who had newly diagnosed adenomatous polyps were interviewed for information on the history of colorectal cancer in their parents and siblings, The risk of colorectal cancer in family members was analyzed according to the characteristics of the patients with adenomas and in comparison with a sample of patients' spouses, who served as controls. Results. Among the patients with adenomas, 1199 provided information on whether they had a family history of colorectal cancer. After the exclusion of families for which information was incomplete and of 48 patients who had been referred for colonoscopy solely because they had a family history of colorectal cancer, there were 1031 patients with adenomas, 1865 parents, 2381 siblings, and 1411 spouse controls. The relative risk of colorectal cancer, adjusted for the year of birth and sex, was 1.78 for the parents and siblings of the patients with adenomas as compared with the spouse controls (95 percent confidence interval, 1.18 to 2.67). The relative risk for siblings of patients in whom adenomas were diagnosed before 60 years of age was 2.59 (95 percent confidence interval, 1.46 to 4.58), as compared with the siblings of patients who were 60 or older at the time of diagnosis and after adjustment for the sibling's year of birth and sex and a parental history of colorectal cancer. The risk increased with decreasing age at the time of the diagnosis of adenoma (P for trend <0.001). The relative risk for the siblings of patients who had a parent with colorectal cancer, as compared with those who had no parent with cancer, was 3.25 (95 percent confidence interval, 1.92 to 5.52), after adjustment for the sibling's year of birth and sex and the patient's age at diagnosis. Conclusions. Siblings and parents of patients with adenomatous polyps are at increased risk for colorectal cancer, particularly when the adenoma is diagnosed before the age of 60 or - in the case of siblings - when a parent has had colorectal cancer. C1 BOSTON CITY HOSP,MALLORY INST PATHOL,BOSTON,MA. VET AFFAIRS MED CTR,MINNEAPOLIS,MN. MT SINAI HOSP,NEW YORK,NY. VALLEY PRESBYTERIAN HOSP,VAN NUYS,CA. CEDARS SINAI MED CTR,LOS ANGELES,CA. MASSACHUSETTS GEN HOSP,BOSTON,MA. MILWAUKEE CTY MED CTR,MILWAUKEE,WI. UNIV UTAH,SALT LAKE CITY,UT. IMPERIAL CANC RES FUND,LEEDS,W YORKSHIRE,ENGLAND. RP Winawer, SJ (reprint author), MEM SLOAN KETTERING CANC CTR,DEPT MED,SERV GASTROENTEROL & NUTR,NATL POLYP STUDY HEADQUARTERS,NEW YORK,NY 10021, USA. OI Bishop, Tim/0000-0002-8752-8785 FU NCI NIH HHS [CA 46940] NR 44 TC 204 Z9 209 U1 2 U2 5 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 11 PY 1996 VL 334 IS 2 BP 82 EP 87 DI 10.1056/NEJM199601113340204 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA TP363 UT WOS:A1996TP36300004 PM 8531963 ER PT J AU Listman, JA Rimm, IJ Wang, YS Geller, MC Tang, JC Ho, S Finn, PW Perkins, DL AF Listman, JA Rimm, IJ Wang, YS Geller, MC Tang, JC Ho, S Finn, PW Perkins, DL TI Plasticity of the T cell receptor repertoire in TCR beta-chain transgenic mice SO CELLULAR IMMUNOLOGY LA English DT Article ID ANTIGEN RECEPTOR; ALPHA-CHAINS; RECOGNITION; GENE; MHC; ANTIBODIES; SELECTION; PEPTIDE; REGION; ALLORECOGNITION AB The potential alpha beta T cell receptor (TCR) repertoire in normal mice is extremely large and estimated by M, M. Davis and P. J. Bjorkman (Nature 334, 395, 1988) to include 5,2 x 10(18) different receptor molecules. This tremendous diversity provides the basis for T cell recognition of the universe of antigens including bacterial, viral, and allogeneic epitopes. Expression of a single TCR beta-chain transgene should alter the repertoire by limiting the available diversity, therefore, creating holes in the repertoire or producing TCR with decreased affinity. To determine the effect of drastically decreasing the size of the repertoire, we investigated T cell responses in TCR beta-chain transgenic mice expressing the V beta 8.2 transgene. Previous results showed that > 98% of T cells in these mice express the transgene; thus, the TCR repertoire is reduced by orders of magnitude. We tested the T cell responses of the transgenic mice and nontransgenic littermates to nine different MHC haplotypes in mixed lymphocyte reactions, five protein antigens, and eight immunogenic peptides. Surprisingly, the transgenic mice responded to all antigenic stimuli tested indicating the lack of a hole in the TCR repertoire. Interestingly, however, the response in every case was quantitatively lower than the response by the nontransgenic littermates. In contrast, transgenic and nontransgenic T cells responded equivalently to stimulation with mitogens or to stimulation with immobilized alpha-TCR mAb indicating that the transgenic T cells had a normal capacity to respond. To differentiate between decreased TCR affinity and decreased precursor frequency, we performed a limiting dilution analysis to the peptide antigens CI:NP and OVA(324-339). The results showed approximately a three-to eight-fold decrease in the frequency of transgenic T cells responding to the peptide compared to nontransgenic littermates. We previously showed that the response to CI84-98 and PLP could be blocked with anti-V beta 8 mAb indicating that V beta 8.2-bearing T cells are capable of responding to peptide antigen, Analysis of TCR V alpha chain expression by PCR and how cytometry showed similar V alpha expression in both the transgenic and the nontransgenic mice. These results demonstrate tremendous plasticity in the TCR repertoire permitting T cell responses by the transgenic mice to all antigens tested. However, the decreased magnitude of the responses may impair the capacity to defend against natural pathogens. Therefore, although the large TCR repertoire of normal mice may not be necessary to produce in vibro responses to many experimental antigens, it may confer survival benefits in natural environments. (C) 1996 Academic Press, Inc. C1 BRIGHAM & WOMENS HOSP,LAB IMMUNOGENET & TRANSPLANTAT,BOSTON,MA 02115. CHILDRENS HOSP,DEPT MED,DIV RENAL,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,DEPT MED,COMBINED PULM DIV,BOSTON,MA 02115. BETH ISRAEL HOSP,BOSTON,MA 02115. DANA FARBER CANC INST,DIV PEDIAT HEMATOL ONCOL,DEPT MED,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. FU NCI NIH HHS [P01 CA39542]; NIAID NIH HHS [AI-31517]; NICHD NIH HHS [T32HD07268] NR 37 TC 14 Z9 15 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0008-8749 J9 CELL IMMUNOL JI Cell. Immunol. PD JAN 10 PY 1996 VL 167 IS 1 BP 44 EP 55 DI 10.1006/cimm.1996.0006 PG 12 WC Cell Biology; Immunology SC Cell Biology; Immunology GA TQ809 UT WOS:A1996TQ80900006 PM 8548844 ER PT J AU Young, LHY AF Young, LHY TI Therapy for cytomegalovirus retinitis: Still no silver lining SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Editorial Material ID GANCICLOVIR RP Young, LHY (reprint author), HARVARD UNIV,MASSACHUSETTS EYE & EAR INFIRM,SCH MED,243 CHARLES ST,BOSTON,MA 02114, USA. NR 10 TC 3 Z9 3 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JAN 10 PY 1996 VL 275 IS 2 BP 149 EP 150 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA TN280 UT WOS:A1996TN28000034 PM 8531313 ER PT J AU Aveline, BM Kochevar, IE Redmond, RW AF Aveline, BM Kochevar, IE Redmond, RW TI N-hydroxypyridine-2(1H)-thione: Not a selective generator of hydroxyl radicals in aqueous solution SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID FLASH-PHOTOLYSIS; ENERGY-TRANSFER; N-HYDROXY-2-THIOPYRIDONE; BENZOPHENONE; REACTIVITY C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. NR 20 TC 28 Z9 28 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD JAN 10 PY 1996 VL 118 IS 1 BP 289 EP 290 DI 10.1021/ja953293i PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA TQ161 UT WOS:A1996TQ16100061 ER PT J AU Scherer, PE Okamoto, T Chun, MY Nishimoto, I Lodish, HF Lisanti, MP AF Scherer, PE Okamoto, T Chun, MY Nishimoto, I Lodish, HF Lisanti, MP TI Identification, sequence, and expression of caveolin-2 defines a caveolin gene family SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID TRANSPORT VESICLES; PROTEIN; COMPONENT; MEMBRANE; ADIPOCYTES; VIP21; FORM AB Caveolin, a 21- to 24-kDa integral membrane protein, is a principal component of caveolae membranes, Caveolin interacts directly with heterotrimeric guanine nucleotide binding proteins (G proteins) and can functionally regulate their activity, Here, an approximate to 20-kDa caveolin-related protein, caveolin-2, was identified through microsequencing of adipocyte-derived caveolin-enriched membranes; caveolin was retermed caveolin-1, Caveolins 1 and 2 are similar in most respects. mRNAs for both caveolin-1 and caveolin-2 are most abundantly expressed in white adipose tissue and are induced during adipocyte differentiation. Caveolin-2 colocalizes with caveolin-1, indicating that caveolin-2 also localizes to caveolae, However, caveolin-1 and caveolin-2 differ in their functional interactions with heterotrimeric G proteins, possibly explaining why caveolin-1 and -2 are coexpressed within a single cell. C1 WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,SHRINERS HOSP CRIPPLED CHILDREN,DEPT ANESTHESIA,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP E,CARDIOVASC RES CTR,DEPT MED,BOSTON,MA 02129. MIT,DEPT BIOL,CAMBRIDGE,MA 02139. RI Lisanti, Michael/C-6866-2013 FU NIDDK NIH HHS [DK-47618]; NIGMS NIH HHS [GM-49516, GM-50443] NR 28 TC 415 Z9 424 U1 0 U2 8 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 9 PY 1996 VL 93 IS 1 BP 131 EP 135 DI 10.1073/pnas.93.1.131 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TP367 UT WOS:A1996TP36700026 PM 8552590 ER PT J AU Schacter, DL Alpert, NM Savage, CR Rauch, SL Albert, MS AF Schacter, DL Alpert, NM Savage, CR Rauch, SL Albert, MS TI Conscious recollection and the human hippocampal formation: Evidence from positron emission tomography SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE memory; visual cortex; hippocampus; frontal lobes ID IMPLICIT MEMORY; PET AB We used positron emission tomography (PET) to examine the role of the hippocampal formation in implicit and explicit memory. Human volunteers studied a list of familiar words, and then they either provided the first word that came to mind in response to three-letter cues (implicit memory) or tried to recall studied words in response to the same cues (explicit memory), There was no evidence of hippocampal activation in association with implicit memory, However, priming effects on the implicit memory test were associated with decreased activity in extrastriate visual cortex, On the explicit memory test, subjects recalled many target words in one condition and recalled few words in a second condition, despite trying to remember them. Comparisons between the two conditions showed that blood-flow increases in the hippocampal formation are specifically associated with the conscious recollection of studied words, whereas bloodflow increases in frontal regions are associated with efforts to retrieve target words, Our results help to clarify some puzzles concerning the role of the hippocampal formation in human memory. C1 MASSACHUSETTS GEN HOSP, DEPT RADIOL, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, DEPT PSYCHIAT, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, DEPT NEUROL, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. RP Schacter, DL (reprint author), HARVARD UNIV, DEPT PSYCHOL, 33 KIRKLAND ST, CAMBRIDGE, MA 02138 USA. OI Schacter, Daniel/0000-0002-2460-6061 FU NCI NIH HHS [T32 CA09362]; NIA NIH HHS [R01 AG08441-06]; NIMH NIH HHS [MH01215] NR 34 TC 485 Z9 488 U1 1 U2 9 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JAN 9 PY 1996 VL 93 IS 1 BP 321 EP 325 DI 10.1073/pnas.93.1.321 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TP367 UT WOS:A1996TP36700064 PM 8552630 ER PT J AU Welsh, DK Reppert, SM AF Welsh, DK Reppert, SM TI Gap junctions couple astrocytes but not neurons in dissociated cultures of rat suprachiasmatic nucleus SO BRAIN RESEARCH LA English DT Article DE suprachiasmatic nucleus; gap junction; neuron; astrocyte; cell culture; circadian rhythm ID MOLECULAR-CLONING; CONNEXIN FAMILY; CIRCADIAN CLOCK; BRAIN; PROTEIN; INVITRO; DYE; EXPRESSION; CELLS; NEOCORTEX AB Individual neurons dissociated from rat suprachiasmatic nucleus can express independently phased circadian firing rhythms in culture. The phases of these rhythms are unperturbed by reversible blockade of neuronal firing lasting 2.5 days, indicating that multiple circadian clocks continue to operate in the absence of conventional synaptic transmission. The possibility remains, however, that these circadian rhythms might depend on some other form of intercellular communication. In the present study, a potential role for gap junctional coupling in SCN cultures was evaluated by introduction of the tracer molecule Neurobiotin into both neurons (n = 98) and astrocytes (n = 10), as well as by immunolabeling for specific connexins, the molecular components of gap junctions. Astrocytes were extensively coupled to each other by connexin43-positive gap junctions, but no evidence was found for coupling of neurons to each other or to astrocytes. These data support the hypothesis that neurons expressing independently phased circadian rhythms in SCN cultures ('clock cells') are autonomous, single cell circadian oscillators, but do not exclude a role for glia in synchronizing neuronal clock cells in vivo. C1 MASSACHUSETTS GEN HOSP,LAB DEV CHRONOBIOL,GRJ 1226,BOSTON,MA 02114. HARVARD UNIV,SCH MED,NEUROSCI PROGRAM,BOSTON,MA 02115. RI Welsh, David/G-2277-2011 NR 48 TC 37 Z9 37 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD JAN 8 PY 1996 VL 706 IS 1 BP 30 EP 36 DI 10.1016/0006-8993(95)01172-2 PG 7 WC Neurosciences SC Neurosciences & Neurology GA TR386 UT WOS:A1996TR38600004 PM 8720489 ER PT J AU Platanias, LC Uddin, S Yetter, A Sun, XJ White, MF AF Platanias, LC Uddin, S Yetter, A Sun, XJ White, MF TI The type I interferon receptor mediates tyrosine phosphorylation of insulin receptor substrate 2 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GAMMA SIGNAL-TRANSDUCTION; PHOSPHATIDYLINOSITOL 3'-KINASE; TRANSCRIPTION FACTOR; PROTEIN; IRS-1; ALPHA/BETA; KINASE; CELLS; DOMAINS; PATHWAY AB Binding of interferon alpha (IFN alpha) to its receptor induces activation of the Tyk-2 and Jak-1 tyrosine kinases and tyrosine phosphorylation of multiple downstream signaling elements, including the Stat components of the interferon-stimulated gene factor 3 (ISGF-3). IFN alpha also induces tyrosine phosphorylation of IRS-1, the principle substrate of the insulin receptor. In this study we demonstrate that various Type I IFNs rapidly stimulate tyrosine phosphorylation of IRS-2. This is significant since IRS-2 is the major IRS protein found in hematopoietic cells. The IFN alpha-induced phosphorylated form of IRS-2 associates with the p85 regulatory subunit of the phosphatidylinositol 3'-kinase, suggesting that this kinase participates in an IFN alpha-signaling cascade downstream of IRS-2. We also provide evidence for an interaction of IRS-2 with Tyk-2, suggesting that Tyk-2 is the kinase that phosphorylates this protein during IFN alpha stimulation. A conserved region in the pleckstrin homology domain of IRS-2 may be required for the interaction of IRS-2 with Tyk-2, as shown by the selective binding of glutathione S-transferase (GST) fusion proteins containing the IRS-2-IH1(PH) or IRS-1-IH1(PH) domains to Tyk-2 but not other Janus kinases in vitro. C1 EDWARD HINES VET ADM MED CTR,HINES,IL 60141. HARVARD UNIV,SCH MED,JOSLIN DIABET CTR,DIV RES,BOSTON,MA 02215. RP Platanias, LC (reprint author), LOYOLA UNIV,DEPT MED,DIV HEMATOL ONCOL,BLDG 112,2160 S 1ST AVE,MAYWOOD,IL 60153, USA. FU NIDDK NIH HHS [DK43808, DK38712] NR 51 TC 109 Z9 110 U1 1 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD JAN 5 PY 1996 VL 271 IS 1 BP 278 EP 282 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TP361 UT WOS:A1996TP36100043 PM 8550573 ER PT J AU Boch, R Mehta, B Connolly, T Durst, T Arnason, JT Redmond, RW Scaiano, JC AF Boch, R Mehta, B Connolly, T Durst, T Arnason, JT Redmond, RW Scaiano, JC TI Singlet oxygen photosensitizing properties of bithiophene and terthiophene derivatives SO JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY LA English DT Article DE singlet oxygen; bithiophene derivatives; tertiophene derivatives ID ALPHA-TERTHIENYL; SUBSTITUTED DERIVATIVES; INFRARED LUMINESCENCE; EFFICIENCY; PHOSPHORESCENCE; THIOPHENES; REACTIVITY; POLYMERS AB An extensive set of thiophene derivatives has been evaluated to determine their sensitized quantum yields for singlet oxygen generation in solution. The values have been determined using laser excitation and time-resolved detection of the near-IR phosphorescence from singlet oxygen. Values determined in this work, cited in earlier publications and thesis dissertations, as well as those in the literature have been combined in an extensive set of tables providing an up-to-date compilation of these efficiencies. These values are independently being used to derive quantitative structure-activity relationships (QSARs) whereby an optimum set of physical parameters for the phototoxicity to a desired target organism may be ascertained. C1 UNIV OTTAWA,DEPT CHEM,OTTAWA,ON K1N 6N5,CANADA. UNIV OTTAWA,DEPT BIOL,OTTAWA,ON K1N 6N5,CANADA. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,BOSTON,MA 02114. NR 27 TC 30 Z9 30 U1 0 U2 8 PU ELSEVIER SCIENCE SA LAUSANNE PI LAUSANNE 1 PA PO BOX 564, 1001 LAUSANNE 1, SWITZERLAND SN 1010-6030 J9 J PHOTOCH PHOTOBIO A JI J. Photochem. Photobiol. A-Chem. PD JAN 4 PY 1996 VL 93 IS 1 BP 39 EP 47 DI 10.1016/1010-6030(95)04144-3 PG 9 WC Chemistry, Physical SC Chemistry GA TW544 UT WOS:A1996TW54400006 ER PT J AU Rosen, FS AF Rosen, FS TI Species and specificity: An interpretation of the history of immunology - Mazumdar,PMH SO NATURE LA English DT Book Review RP Rosen, FS (reprint author), CTR BLOOD RES,800 HUNTINGTON AVE,BOSTON,MA 02115, USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD JAN 4 PY 1996 VL 379 IS 6560 BP 36 EP 36 DI 10.1038/379036a0 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TN216 UT WOS:A1996TN21600045 ER PT J AU Rosen, FS AF Rosen, FS TI Immunology: The making of a modern science - Gallagher,RB, Gilder,J, Nossal,GIV, Salvatore,G SO NATURE LA English DT Book Review RP Rosen, FS (reprint author), CTR BLOOD RES,800 HUNTINGTON AVE,BOSTON,MA 02115, USA. NR 1 TC 1 Z9 1 U1 0 U2 0 PU MACMILLAN MAGAZINES LTD PI LONDON PA 4 LITTLE ESSEX STREET, LONDON, ENGLAND WC2R 3LF SN 0028-0836 J9 NATURE JI Nature PD JAN 4 PY 1996 VL 379 IS 6560 BP 36 EP 36 DI 10.1038/379036a0 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA TN216 UT WOS:A1996TN21600046 ER PT J AU Gervais, DA Whitman, GJ AF Gervais, DA Whitman, GJ TI Ruptured abdominal aortic aneurysm SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article RP Gervais, DA (reprint author), MASSACHUSETTS GEN HOSP,BOSTON,MA 02114, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MASS MEDICAL SOC PI BOSTON PA 10 SHATTUCK, BOSTON, MA 02115 SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD JAN 4 PY 1996 VL 334 IS 1 BP 27 EP 27 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA TM482 UT WOS:A1996TM48200007 PM 7494568 ER PT J AU Teicher, BA Ara, G Menon, K Schaub, RG AF Teicher, BA Ara, G Menon, K Schaub, RG TI In vivo studies with interleukin-12 alone and in combination with monocyte colony-stimulating factor and/or fractionated radiation treatment SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID INTERFERON-GAMMA PRODUCTION; NK CELLS; PROLIFERATION; ANTITUMOR; MATURATION; INDUCTION; CANCER; IL-12; IMMUNOTHERAPY AB Interleukin-12 (IL-12) was found to be an active anti-tumor agent in 3 established murine solid tumors: B16 melanoma, Lewis lung carcinoma and renal cell carcinoma (RenCa). IL-12 was well tolerated over a 100-fold dose range. Only the high-dose treatment of IL-12 resulted in a clear reduction in the number of lung metastases from B16 melanoma and Lewis lung carcinoma. Treatment of animals bearing Lewis lung carcinoma with IL-12 in combination with fractionated radiation therapy was markedly dose-modifying, indicating that IL-12 was acting synergistically with radiation. Treatment of animals bearing the same tumor with monocyte colony-stimulating factor (M-CSF) along with fractionated radiation therapy resulted in a parallel increase in tumor growth delay with increasing dose of M-CSF, indicating that M-CSF was affecting a subpopulation of tumor cells in addition to those killed by radiation therapy. The combination of IL-12 with M-CSF was most effective with radiation therapy, especially in the clinically relevant dosages of 2 and 3 Gy per fraction. By isobologram analysis, IL-12 and M-CSF, along with fractionated radiation therapy, resulted in a greater-than-additive (synergistic) tumor response. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,SCH MED,JOINT CTR RADIAT THERAPY,BOSTON,MA 02115. GENET INST INC,ANDOVER,MA 01810. RP Teicher, BA (reprint author), HARVARD UNIV,SCH MED,DANA FARBER CANC INST,44 BINNEY ST,BOSTON,MA 02115, USA. NR 28 TC 22 Z9 23 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD JAN 3 PY 1996 VL 65 IS 1 BP 80 EP 84 DI 10.1002/(SICI)1097-0215(19960103)65:1<80::AID-IJC14>3.0.CO;2-M PG 5 WC Oncology SC Oncology GA TM773 UT WOS:A1996TM77300014 PM 8543401 ER PT J AU Rasmussen, SA Bieber, FR Benacerraf, BR Lachman, RS Rimoin, DL Holmes, LB AF Rasmussen, SA Bieber, FR Benacerraf, BR Lachman, RS Rimoin, DL Holmes, LB TI Epidemiology of osteochondrodysplasias: Changing trends due to advances in prenatal diagnosis SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE osteochondrodysplasias; skeletal dysplasias; dwarfism; chondrodysplasias; fetus; bone; collagen diseases; prenatal diagnosis; ultrasonography ID BIRTH PREVALENCE RATES; SKELETAL DYSPLASIAS; MALFORMATIONS; MUTATIONS AB The osteochondrodysplasias (skeletal dysplasias) are a heterogeneous group of disorders characterized by abnormalities in cartilage and bone growth and development, Some of these disorders are detectable during the second trimester by sonographic techniques, We ascertained cases of osteochondrodysplasias in elective pregnancy terminations, stillborn infants older than 20 gestational weeks, and liveborn infants diagnosed by the fifth day of life as part of an ongoing active malformation surveillance program, Forty-nine cases of osteochondrodysplasias were identified among approximately 126,000 deliveries at Brigham and Women's Hospital (BWH) during a 15-year period (Feb, 16, 1972-Feb. 15, 1975; Jan, 1, 1979-Dec. 31, 1990), When cases delivered to women who had planned to deliver at another hospital but were transferred for high-risk care (transfers) were excluded, the prevalence rate was 2.14 cases per 10,000 deliveries, During the early period (1972-1975) no cases were suspected prenatally, while during the 1988-1990 period, 80% of all cases and 57% of cases delivered to women who had always planned to deliver at BWH (nontransfers) were suspected by ultrasonography. Birth status changed through our period of surveillance, In the final 3-year period (1988-1990), 40% of all cases and 29% of non-transfers with osteochondrodysplasias were pregnancy terminations, compared to none during the 1972-1975 period, The increasing frequency of pregnancy terminations complicated the diagnosis of these conditions, Despite extensive evaluation, a definitive diagnosis was not possible in 8 of 49 cases (16%), Biochemical and molecular genetic methods of diagnosis will continue to become more important if the current trend of wide utilization of prenatal sonography and termination of affected pregnancies continues, (C) 1996 Wiley-Liss, Inc. C1 MASSACHUSETTS GEN HOSP,CHILDRENS SERV,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT OBSTET & GYNECOL,BOSTON,MA 02114. BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,DEPT OBSTET & GYNECOL,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,DEPT RADIOL,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,DEPT NEWBORN MED,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BOSTON,MA. UNIV CALIF LOS ANGELES,HARBOR MED CTR,STEVEN SPIELBERG PEDIAT RES CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,HARBOR MED CTR,CEDARS SINAI MED CTR,AHMANSON PEDIAT CTR,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. FU NICHD NIH HHS [HD22657] NR 40 TC 53 Z9 54 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD JAN 2 PY 1996 VL 61 IS 1 BP 49 EP 58 DI 10.1002/(SICI)1096-8628(19960102)61:1<49::AID-AJMG10>3.0.CO;2-W PG 10 WC Genetics & Heredity SC Genetics & Heredity GA TM206 UT WOS:A1996TM20600011 PM 8741918 ER PT J AU Berberich, I Shu, G Siebelt, F Woodgett, JR Kyriakis, JM Clark, EA AF Berberich, I Shu, G Siebelt, F Woodgett, JR Kyriakis, JM Clark, EA TI Cross-linking CD40 on B cells preferentially induces stress-activated protein kinases rather than mitogen-activated protein kinases SO EMBO JOURNAL LA English DT Article DE B cells; CD40; JNK; MAPK; SAPK ID C-JUN; TYROSINE PHOSPHORYLATION; DENDRITIC CELLS; SIGNAL; RECEPTOR; COMPLEX; DOMAIN; LIGAND; IDENTIFICATION; EXPRESSION AB The B cell-associated surface molecule CD40 plays a key role in T cell-dependent B cell maturation, as individuals with defects in either CD40 or its ligand are impaired in immunoglobulin isotype class switching and germinal center formation, CD40 signaling activates downstream effecters, including the tyrosine protein kinase, Lyn, the phosphatidylinositol-3-kinase (PI-3 kinase), and the transcription factor, NF-kappa B. In this study, we demonstrate that stress-activated protein kinases (SAPK) are activated after CD40 cross-linking on various B cell lines or human tonsillar B cells. The activation is rapid and transient and is mediated through a cyclosporin A-insensitive pathway, Furthermore, this signaling pathway appears not to rely on protein kinase C, While CD40 ligation strongly activates the SAPKs (up to 25-fold), it does not affect members of the mitogen-activated protein kinase family (MAPK; ERK1 and ERK2), Consistent with these data, CD40 signals up-regulate c-jun but not c-fos mRNA and alter the transcription factor ATF2 but not the Raf-1 protein, In summary, CD40 signaling preferentially induces SAPK but not MAPK. C1 UNIV WASHINGTON,MED CTR,REG PRIMATE RES CTR,SEATTLE,WA 98195. UNIV WASHINGTON,MED CTR,DEPT MICROBIOL,SEATTLE,WA 98195. PRINCESS MARGARET HOSP,ONTARIO CANC INST,TORONTO,ON M4X 1K9,CANADA. MASSACHUSETTS GEN HOSP EAST,DIABET RES LAB,BOSTON,MA 02129. RI Woodgett, Jim/F-1087-2010; Clark, Edward/K-3462-2012 OI Woodgett, Jim/0000-0003-3731-5797; FU NCRR NIH HHS [RR00166]; NIGMS NIH HHS [GM37905] NR 70 TC 163 Z9 165 U1 1 U2 3 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0261-4189 J9 EMBO J JI Embo J. PD JAN 2 PY 1996 VL 15 IS 1 BP 92 EP 101 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA TQ160 UT WOS:A1996TQ16000010 PM 8598210 ER PT S AU Papisov, M Garrido, L Poss, K Wright, C Weissleder, R Brady, TJ AF Papisov, M Garrido, L Poss, K Wright, C Weissleder, R Brady, TJ GP CONTROLLED RELEASE SOC TI A long-circulating polymer with hydrolizable main chain SO 23RD INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS, 1996 PROCEEDINGS SE CONTROLLED RELEASE SOCIETY. INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS LA English DT Proceedings Paper CT 23rd International Symposium on Controlled Release of Bioactive Materials CY JUL 07-10, 1996 CL KYOTO, JAPAN SP Controlled Release Soc Inc, Japan Soc Drug Delivery Syst RP Papisov, M (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIOL,NMR CTR,CHARLESTOWN,MA 02129, USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU CONTROLLED RELEASE SOCIETY INC PI DEERFIELD PA 1020 MILWAUKEE AVE SUITE 235, DEERFIELD, IL 60015 SN 1022-0178 J9 CRS BUI NAT PY 1996 BP 107 EP 108 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA BH42C UT WOS:A1996BH42C00055 ER PT S AU Trubetskoy, VS Torchilin, VP Wolf, GL AF Trubetskoy, VS Torchilin, VP Wolf, GL GP CONTROLLED RELEASE SOC TI Massage-induced delivery of subcutaneously-injected liposome-encapsulated drugs into the blood. SO 23RD INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS, 1996 PROCEEDINGS SE CONTROLLED RELEASE SOCIETY. INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS LA English DT Proceedings Paper CT 23rd International Symposium on Controlled Release of Bioactive Materials CY JUL 07-10, 1996 CL KYOTO, JAPAN SP Controlled Release Soc Inc, Japan Soc Drug Delivery Syst RP Trubetskoy, VS (reprint author), MASSACHUSETTS GEN HOSP,CTR IMAGING & PHARMACEUT RES,BOSTON,MA 02114, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CONTROLLED RELEASE SOCIETY INC PI DEERFIELD PA 1020 MILWAUKEE AVE SUITE 235, DEERFIELD, IL 60015 SN 1022-0178 J9 CRS BUI NAT PY 1996 BP 403 EP 404 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA BH42C UT WOS:A1996BH42C00205 ER PT S AU Papisov, M LaPointe, L Myagchilov, S Poss, K Weissleder, R Brady, TJ AF Papisov, M LaPointe, L Myagchilov, S Poss, K Weissleder, R Brady, TJ GP CONTROLLED RELEASE SOC TI Underlying mechanisms of surface protection by molecular brushes: Models and reality SO 23RD INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS, 1996 PROCEEDINGS SE CONTROLLED RELEASE SOCIETY. INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS LA English DT Proceedings Paper CT 23rd International Symposium on Controlled Release of Bioactive Materials CY JUL 07-10, 1996 CL KYOTO, JAPAN SP Controlled Release Soc Inc, Japan Soc Drug Delivery Syst RP Papisov, M (reprint author), MASSACHUSETTS GEN HOSP,NMR CTR,CHARLESTOWN,MA 02129, USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU CONTROLLED RELEASE SOCIETY INC PI DEERFIELD PA 1020 MILWAUKEE AVE SUITE 235, DEERFIELD, IL 60015 SN 1022-0178 J9 CRS BUI NAT PY 1996 BP 681 EP 682 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA BH42C UT WOS:A1996BH42C00344 ER PT S AU Trubetskoy, VS FrankKamenetsky, MD Torchilin, VP AF Trubetskoy, VS FrankKamenetsky, MD Torchilin, VP GP CONTROLLED RELEASE SOC TI Polyoxyethylene-lipid micelles loaded with solubilized amphiphilic compounds: Interaction with serum components SO 23RD INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS, 1996 PROCEEDINGS SE CONTROLLED RELEASE SOCIETY. INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS LA English DT Proceedings Paper CT 23rd International Symposium on Controlled Release of Bioactive Materials CY JUL 07-10, 1996 CL KYOTO, JAPAN SP Controlled Release Soc Inc, Japan Soc Drug Delivery Syst RP Trubetskoy, VS (reprint author), MASSACHUSETTS GEN HOSP,CTR IMAGING & PHARMACEUT RES,CHARLESTOWN,MA 02129, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CONTROLLED RELEASE SOCIETY INC PI DEERFIELD PA 1020 MILWAUKEE AVE SUITE 235, DEERFIELD, IL 60015 SN 1022-0178 J9 CRS BUI NAT PY 1996 BP 781 EP 782 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA BH42C UT WOS:A1996BH42C00394 ER PT S AU Hoop, B Torchilin, VP AF Hoop, B Torchilin, VP GP CONTROLLED RELEASE SOC TI Theoretical considerations on self-similarity of the polyethylene glycol-coated liposomal surface SO 23RD INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS, 1996 PROCEEDINGS SE CONTROLLED RELEASE SOCIETY. INTERNATIONAL SYMPOSIUM ON CONTROLLED RELEASE OF BIOACTIVE MATERIALS LA English DT Proceedings Paper CT 23rd International Symposium on Controlled Release of Bioactive Materials CY JUL 07-10, 1996 CL KYOTO, JAPAN SP Controlled Release Soc Inc, Japan Soc Drug Delivery Syst RP Hoop, B (reprint author), MASSACHUSETTS GEN HOSP,CTR IMAGING & PHARMACEUT RES,CHARLESTOWN,MA 02129, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU CONTROLLED RELEASE SOCIETY INC PI DEERFIELD PA 1020 MILWAUKEE AVE SUITE 235, DEERFIELD, IL 60015 SN 1022-0178 J9 CRS BUI NAT PY 1996 BP 785 EP 786 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA BH42C UT WOS:A1996BH42C00396 ER PT B AU Warshaw, A AF Warshaw, A BE Cavallari, A Mazziotti, A Principe, A TI Lessons learned from experimental models of acute pancreatitis SO 2ND WORLD CONGRESS - INTERNATIONAL HEPATO-PANCREATO-BILIARY ASSOCIATION, VOL II: BILIARY-PANCREAS LA English DT Proceedings Paper CT 2nd World Congress of the International-Hepato-Pancreato-Biliary-Association CY JUN 02-06, 1996 CL BOLOGNA, ITALY SP Int Hepato Pancreato Biliary Assoc C1 MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. NR 0 TC 1 Z9 1 U1 0 U2 0 PU MONDUZZI EDITORE PI 40128 BOLOGNA PA VIA FERRARESE 119/2, 40128 BOLOGNA, ITALY BN 88-323-0602-6 PY 1996 BP 967 EP 972 PG 6 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA BG40Q UT WOS:A1996BG40Q00084 ER PT B AU Mester, M Tompkins, RG Gelfand, JA Dinarello, CA Clark, BD Burke, JF AF Mester, M Tompkins, RG Gelfand, JA Dinarello, CA Clark, BD Burke, JF BE Faist, E TI Interleukin-1 alpha tissue levels during endotoxemia in the rat SO 4TH INTERNATIONAL CONGRESS ON THE IMMUNE CONSEQUENCES OF TRAUMA, SHOCK AND SEPSIS: MECHANISMS AND THERAPEUTIC APPROACHES LA English DT Proceedings Paper CT 4th International Congress on the Immune Consequences of Trauma, Shock and Sepsis - Mechanisms and Therapeutic Approaches CY MAR 04-08, 1997 CL MUNICH, GERMANY AB We performed a dose-response study of Interleukin-1 alpha production during endotoxemia in the rat. Sprague-Dawley rats were infused with 15 mg/Kg. 1.5 mg/Kg, or 0.15 mg/Kg E.coli 0111:B4 LPS, or saline I.V. Unmanipulated rats were baseline normal controls, The whole spleen, lung, small bowel, thymus, kidneys, as well as samples of liver and skin were harvested at various time-points within the first 24 hours. Tissues were assayed with a radioimmunoassay specific for rat IL-1 alpha (detection limit 150 pg/mL). IL-1 alpha was expressed as pg/mg total protein (TP) +/- SEM. IL-1 alpha was constitutively present only in the skin. LPS challenge induced dramatic biphasic elevation of IL-1 alpha in a dose-response pattern in all organs tested except for the kidney and pancreas. The spleen was the most responsive organ to LPS-induced IL-1 alpha production. Maximal tolerable LPS dose for rats in this study was between 1.5-15 mg/Kg as the highest LPS dose caused 100% mortality whereas the mid-dose caused none. This data may prove useful to further understand the effects of endotoxemia, as well as to better plan in vivo IL-1 agonist-blockade protocols. RP Mester, M (reprint author), MASSACHUSETTS GEN HOSP,SURG SERV,BOSTON,MA 02114, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MONDUZZI EDITORE PI 40128 BOLOGNA PA VIA FERRARESE 119/2, 40128 BOLOGNA, ITALY BN 88-323-0304-3 PY 1996 BP 1009 EP 1014 PG 6 WC Emergency Medicine; Immunology; Medicine, General & Internal SC Emergency Medicine; Immunology; General & Internal Medicine GA BH98U UT WOS:A1996BH98U00180 ER PT B AU Lees, S Hanson, DB Page, EA AF Lees, S Hanson, DB Page, EA BE Tortoli, P Masotti, L TI Mapping the continuous distribution of sonic velocity and elastic modulus in a cross-section of bone SO ACOUSTICAL IMAGING, VOL 22 SE ACOUSTICAL IMAGING LA English DT Proceedings Paper CT 22nd International Symposium on Acoustical Imaging CY SEP 03-07, 1995 CL FLORENCE, ITALY SP Univ Florence, CNR, Area Ric, CESVIT, Reg Toscana, Reg EsaOte Biomed C1 FORSYTH DENT CTR,DEPT BIOENGN,BOSTON,MA 02115. NR 0 TC 1 Z9 1 U1 0 U2 1 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45364-9 J9 ACOUST IMAG PY 1996 VL 22 BP 319 EP 322 PG 4 WC Acoustics; Engineering, Multidisciplinary; Engineering, Biomedical; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Engineering; Radiology, Nuclear Medicine & Medical Imaging GA BF85N UT WOS:A1996BF85N00051 ER PT J AU Weitgasser, R Davalli, AM Capotorto, JV Finegood, DT BonnerWeir, S Weir, GC AF Weitgasser, R Davalli, AM Capotorto, JV Finegood, DT BonnerWeir, S Weir, GC TI Islet transplantation in diabetic Lewis rats a comparison of the transplantation sites kidney and spleen capsule SO ACTA MEDICA AUSTRIACA LA English DT Article DE islet transplantation; transplantation sites; Lewis rats ID BETA-CELL MASS; PANCREATIC-ISLETS; REPLICATION; ISOGRAFTS; SURVIVAL; LIVER; MICE AB Animal studies have been used to investigate different transplantation (Tx) sites to find best conditions for Tx in humans. A few long-term experiments of comparisons of different Tx-sites have been published. Therefore the aim of our study was to compare islet grafts transplanted under the kidney capsule (KTx) of male Lewis rats with grafts transplanted under the spleen capsule (STx) and to observe these animals over a period of 6 months. Diabetes was induced by Streptozotocin and rats were each transplanted with 2000 syngeneic islets under the kidney respectively the spleen capsule. 2 weeks after Tx all animals were normoglycemic. Over the following 6 months nearly normal plasma glucose levels could be maintained in the KTx group whereas the STx animals already became diabetic after 3 months. An oral glucose load after 2 months showed slightly elevated plasma glucose levels in the KTx group which only gained statistical significance in a similar test after 6 months. In the STx group definitely impaired glucose tolerance already prevailed at 2 months. An i.v. glucose tolerance test performed 6 months after Tx showed a loss of first phase insulin release in both Tx groups but an increased second phase release and a preserved response to L-arginine compared to controls. Insulin content remained unchanged in the KTx group, but was markedly reduced in the STx group after 6 months. In conclusion we may say that KTx is able to establish near-normoglycemia in streptozotocin-diabetic Lewis rats which can be maintained over a long period of time despite abnormal glucose tolerance and impaired insulin secretion. STx could normalize plasma glucose only up to 3 months showing early impairment of glucose and insulin regulation. If KTx has immunological or local advantages (islet vascularization, innervation) compared to STx with higher dispersion of islets in spleen and portal vascular system remains unclear. C1 HARVARD UNIV,SCH MED,JOSLIN DIABET CTR,SECT ISLET TRANSPLANTAT & CELL BIOL,BOSTON,MA. FU NIDDK NIH HHS [DK-36836] NR 17 TC 4 Z9 4 U1 1 U2 2 PU BLACKWELL WISSENSCHAFTS-VERLAG GMBH PI BERLIN PA KURFURSTENDAMM 57, D-10707 BERLIN, GERMANY SN 0303-8173 J9 ACTA MED AUST JI Acta Med. Austriaca PY 1996 VL 23 IS 5 BP 156 EP 159 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA WG941 UT WOS:A1996WG94100003 PM 9082744 ER PT J AU Meyer, JS Shirai, T Mortel, KF Muramatsu, K Akiyama, H AF Meyer, JS Shirai, T Mortel, KF Muramatsu, K Akiyama, H TI Testing Xe/CT CBF cerebrovascular reserve for identifying migraine and differentiating Alzheimer's from vascular dementia SO ACTA NEUROLOGICA SCANDINAVICA LA English DT Article; Proceedings Paper CT 3rd International Conference on Xenon/CT CBF CY JUN 25-28, 1995 CL COPENHAGEN, DENMARK C1 BAYLOR COLL MED,DEPT NEUROL,HOUSTON,TX 77030. RP Meyer, JS (reprint author), DEPT VET AFFAIRS MED CTR,CEREBROVASC RES LABS,HOUSTON,TX, USA. OI Akiyama, Hisanao/0000-0003-4491-6064 NR 0 TC 0 Z9 0 U1 0 U2 0 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0001-6314 J9 ACTA NEUROL SCAND JI Acta Neurol. Scand. PY 1996 VL 93 SU 166 BP 148 EP 149 PG 2 WC Clinical Neurology SC Neurosciences & Neurology GA UP136 UT WOS:A1996UP13600056 ER PT J AU Raymond, GV Bauman, ML Kemper, TL AF Raymond, GV Bauman, ML Kemper, TL TI Hippocampus in autism: A Golgi analysis SO ACTA NEUROPATHOLOGICA LA English DT Note DE autism; hippocampus ID EARLY INFANTILE-AUTISM; HYPOPLASIA; BRAIN AB Autism is a behaviorally defined syndrome in which neuropathological abnormalities have been identified in the limbic system and cerebellum. The morphology of hippocampal neurons in two cases of infantile autism was studied and compared to age-matched controls. CA4 neurons in autistic children were smaller in perikaryon area and dendritic branching of both CA4 and CAI neurons was less than in controls. These findings are consistent with previous studies and suggest a curtailment in maturation in the pathogenesis of autism. C1 MASSACHUSETTS GEN HOSP,CHILDRENS NEUROL SERV,BOSTON,MA 02114. BOSTON CITY HOSP,DEPT NEUROL,BOSTON,MA 02118. RP Raymond, GV (reprint author), KENNEDY KRIEGER INST,NEUROGENET UNIT,707 N BROADWAY,BALTIMORE,MD 21205, USA. NR 17 TC 173 Z9 177 U1 4 U2 12 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0001-6322 J9 ACTA NEUROPATHOL JI Acta Neuropathol. PD JAN PY 1996 VL 91 IS 1 BP 117 EP 119 PG 3 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA TL369 UT WOS:A1996TL36900018 PM 8773156 ER PT J AU Kimura, RS Trehey, JA Hutta, J AF Kimura, RS Trehey, JA Hutta, J TI Vein of the vestibular aqueduct in the gerbil SO ACTA OTO-LARYNGOLOGICA LA English DT Article DE gerbil; vein of the vestibular aqueduct; posterior canal crista ID ENDOLYMPHATIC SAC; HYDROPS AB The vein of the vestibular aqueduct in the gerbil runs along the lateral side of the endolymphatic duct, deviates from this course to enter the middle ear cavity, and connects with the lateral sinus. In 20 animals, the vein of the vestibular aqueduct was obliterated by drilling from the middle ear side. Histopathology of these specimens after 2 months' surivival revealed consistent sensory cell atrophy in the posterior canal cristae, frequent loss of sensory cells in a small superior portion of the macula sacculi and in the basal end of the cochlea, and fibrosis and osteogenesis in the three semicircular canals. The endolymphatic sacs contained colloidal substances in some specimens which were otherwise normal. Endolymphatic hydrops was absent except in some specimens which showed additional surgical damage to the endolymphatic sac and canals. The blocked vein re-opened occasionally and connected with the vessel formed in new bone from which it attached to the lateral sinus. The sensory cell degeneration and canal fibrosis reflects the pattern of blood drainage by the vein of the vestibular aqueduct. Vascular disorder in the vestibular labyrinth initiates vestibular symptoms; however, it will not produce endolymphatic hydrops unless function of the endolymphatic sac is impaired. C1 HARVARD UNIV,SCH MED,DEPT OTOL & LARYNGOL,BOSTON,MA 02115. RP Kimura, RS (reprint author), MASSACHUSETTS EYE & EAR INFIRM,ELECTRON MICROSCOPY LAB,DEPT OTOLARYNGOL,243 CHARLES ST,BOSTON,MA 02114, USA. NR 23 TC 8 Z9 8 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0001-6489 J9 ACTA OTO-LARYNGOL JI Acta Oto-Laryngol. PD JAN PY 1996 VL 116 IS 1 BP 44 EP 51 DI 10.3109/00016489609137711 PG 8 WC Otorhinolaryngology SC Otorhinolaryngology GA TR967 UT WOS:A1996TR96700008 PM 8820349 ER PT J AU Schneider, N Nilsson, F Franzon, M Olmstead, R Mody, FV Doan, K AF Schneider, N Nilsson, F Franzon, M Olmstead, R Mody, FV Doan, K TI Nicotine inhaler in smoking cessation: A pilot SO ADDICTION LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 2 Z9 2 U1 0 U2 0 PU CARFAX PUBL CO PI ABINGDON PA PO BOX 25, ABINGDON, OXFORDSHIRE, ENGLAND OX14 3UE SN 0965-2140 J9 ADDICTION JI Addiction PD JAN PY 1996 VL 91 IS 1 BP 143 EP 143 PG 1 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA TQ693 UT WOS:A1996TQ69300042 ER PT J AU Greene, RW AF Greene, RW TI Students with attention-deficit hyperactivity disorder and their teachers - Implications of it goodness-of-fit perspective SO ADVANCES IN CLINICAL CHILD PSYCHOLOGY, VOL 18 SE ADVANCES IN CLINICAL CHILD PSYCHOLOGY LA English DT Article ID BEHAVIORAL INTERVENTIONS; STIMULANT MEDICATION; CONDUCT DISORDER; CHILD-BEHAVIOR; ADHD CHILDREN; CLASSROOM; ACCEPTABILITY; METHYLPHENIDATE; DEPRESSION; THERAPY C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. RP Greene, RW (reprint author), MASSACHUSETTS GEN HOSP,PEDIAT PSYCHOPHARMACOL UNIT,BOSTON,MA 02114, USA. NR 88 TC 8 Z9 8 U1 1 U2 2 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0149-4732 J9 ADV CLIN CHILD PSYCH PY 1996 VL 18 BP 205 EP 230 PG 26 WC Psychology, Clinical SC Psychology GA BE71W UT WOS:A1996BE71W00006 ER PT S AU Potts, JT AF Potts, JT BE Schrier, RW TI Hyperparathyroidism and other hypercalcemic disorders SO ADVANCES IN INTERNAL MEDICINE, VOL 41 SE ADVANCES IN INTERNAL MEDICINE LA English DT Review ID PRIMARY HYPER-PARATHYROIDISM; ENDOCRINE NEOPLASIA TYPE-1; FAMILIAL HYPOCALCIURIC HYPERCALCEMIA; HUMORAL HYPERCALCEMIA; BLOOD-PRESSURE; FOLLOW-UP; REVERSIBLE HYPERTENSION; BENIGN HYPERCALCEMIA; CANDIDATE ONCOGENE; SURGICAL-TREATMENT RP Potts, JT (reprint author), HARVARD UNIV, SCH MED, MASSACHUSETTS GEN HOSP, DEPT MED, BOSTON, MA 02115 USA. NR 246 TC 13 Z9 13 U1 0 U2 0 PU MOSBY, INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146 USA SN 0065-2822 BN 0-8151-8314-3 J9 ADV INTERNAL MED JI Adv.Intern.Med. PY 1996 VL 41 BP 165 EP 212 PG 48 WC Medicine, General & Internal SC General & Internal Medicine GA BF11R UT WOS:A1996BF11R00005 PM 8903589 ER PT S AU Kahn, CR AF Kahn, CR BE Schrier, RW TI New concepts in the pathogenesis of diabetes mellitus SO ADVANCES IN INTERNAL MEDICINE, VOL 41 SE ADVANCES IN INTERNAL MEDICINE LA English DT Review ID ISLET-CELL ANTIBODIES; INSULIN-RECEPTOR SUBSTRATE-1; GLUCOSE-TRANSPORT SYSTEM; BETA-CELL; PIMA-INDIANS; SIGNAL TRANSDUCTION; FOLLOW-UP; RESISTANCE; GENE; MUSCLE RP HARVARD UNIV, SCH MED, DEPT MED, JOSLIN DIABET CTR, RES DIV, BOSTON, MA 02115 USA. NR 123 TC 7 Z9 7 U1 0 U2 1 PU MOSBY, INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146 USA SN 0065-2822 BN 0-8151-8314-3 J9 ADV INTERNAL MED JI Adv.Intern.Med. PY 1996 VL 41 BP 285 EP 321 PG 37 WC Medicine, General & Internal SC General & Internal Medicine GA BF11R UT WOS:A1996BF11R00008 PM 8903592 ER PT S AU Huston, JS Margolies, MN Haber, E AF Huston, JS Margolies, MN Haber, E BE Haber, E TI Antibody binding sites SO ADVANCES IN PROTEIN CHEMISTRY, VOL 49: ANTIGEN BINDING MOLECULES: ANTIBODIES AND T-CELL RECEPTORS SE ADVANCES IN PROTEIN CHEMISTRY LA English DT Review ID SINGLE-CHAIN FV; CHIMERIC PLASMINOGEN-ACTIVATOR; FRAGMENT-D-DIMER; FIBRIN-SPECIFIC ANTIBODY; AMINO-ACID SUBSTITUTION; RABBIT IMMUNOGLOBULIN-G; BISPECIFIC MONOCLONAL-ANTIBODIES; PARA-AZOPHENYLARSONATE FAB-36-71; MULTIVALENT IMMUNE-COMPLEXES; ACUTE MYOCARDIAL-INFARCTION C1 MASSACHUSETTS GEN HOSP, DEPT SURG, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, DEPT MED, BOSTON, MA 02115 USA. HARVARD UNIV, SCH PUBL HLTH, CARDIOVASC BIOL LAB, BOSTON, MA 02115 USA. RP Huston, JS (reprint author), CREAT BIOMOL INC, HOPKINTON, MA 01748 USA. FU NCI NIH HHS [CA39870, R01 CA24432, UO1 CA51880] NR 317 TC 22 Z9 22 U1 0 U2 1 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0065-3233 BN 0-12-034249-9 J9 ADV PROTEIN CHEM JI Adv.Protein Chem. PY 1996 VL 49 BP 329 EP 450 DI 10.1016/S0065-3233(08)60493-3 PG 122 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA BG71H UT WOS:A1996BG71H00007 PM 8908302 ER PT S AU OBrien, CP AF OBrien, CP BE Holtz, A TI Is there an abuse potential for caffeine-containing analgesic combinations? SO ADVANCES IN THE MANAGEMENT OF ACUTE PAIN SE ROYAL SOCIETY OF MEDICINE INTERNATIONAL CONGRESS AND SYMPOSIUM SERIES LA English DT Proceedings Paper CT Symposium on Advances in the Management of Acute Pain CY MAR, 1996 CL MONTERREY, MEXICO SP Procter & Gamble Co C1 UNIV PENN,PHILADELPHIA VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ROYAL SOC MEDICINE PRESS LTD PI LONDON PA 1 WIMPOLE STREET, LONDON, ENGLAND W1M 8AE SN 0142-2367 BN 1-85315-292-7 J9 ROY SOC MED INT CONG PY 1996 IS 218 BP 119 EP 127 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA BG55W UT WOS:A1996BG55W00009 ER PT J AU Woody, G AF Woody, G TI The challenge of dual diagnosis SO ALCOHOL HEALTH & RESEARCH WORLD LA English DT Article DE dual diagnosis; AOD dependence; comorbidity; behavioral and mental disorder; diagnostic criteria; prevalence; etiology; diagnosis; health care delivery; treatment program; treatment outcome ID CLINICAL IMPLICATIONS; MENTAL-DISORDERS; ALCOHOLISM; DEPRESSION; COMORBIDITY; DISTINCTION; ANXIETY; ILLNESS AB Researchers have made great strides in understanding and treating alcoholics with co-occurring psychiatric disorders, improved diagnostic criteria are available, and research has demonstrated that both disorders must be addressed if the dually diagnosed patient is to have the best chance for a good outcome, The best type of treatment program is an integrated approach, assuring that treatments will be coordinated for best effect Additional research is needed to match optimum treatment approaches with cost-effective reimbursement practices. C1 PHILADELPHIA VET AFFAIRS MED CTR,SUBST ABUSE TREATMENT UNIT,PHILADELPHIA,PA. RP Woody, G (reprint author), UNIV PENN,PHILADELPHIA,PA 19104, USA. NR 32 TC 16 Z9 16 U1 1 U2 3 PU NATL INST ALCOHOL ABUSE ALCOHOLISM PI ROCKVILLE PA 6000 EXECUTIVE BLVD, ROCKVILLE, MD 20892-7003 SN 0090-838X J9 ALCOHOL HEALTH RES W JI Alcohol Health Res. World PY 1996 VL 20 IS 2 BP 76 EP 80 PG 5 WC Substance Abuse SC Substance Abuse GA XW451 UT WOS:A1996XW45100001 ER PT J AU Brezinski, ME Tearney, GJ Bouma, BE Boppart, SA Hee, MR Swanson, EA Southern, JF Fujimoto, JG AF Brezinski, ME Tearney, GJ Bouma, BE Boppart, SA Hee, MR Swanson, EA Southern, JF Fujimoto, JG TI Imaging of coronary artery microstructure (in vitro) with optical coherence tomography SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article C1 MIT,ELECTR RES LAB,CAMBRIDGE,MA 02139. MASSACHUSETTS GEN HOSP,CARDIAC UNIT,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. RP Brezinski, ME (reprint author), MIT,DEPT COMP SCI & ELECT ENGN,CAMBRIDGE,MA 02139, USA. RI Boppart, Stephen/C-7338-2009 FU NEI NIH HHS [9-R01-EY11289-10]; NIGMS NIH HHS [9-R01-GM35459-09] NR 5 TC 93 Z9 94 U1 0 U2 2 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD JAN 1 PY 1996 VL 77 IS 1 BP 92 EP 93 DI 10.1016/S0002-9149(97)89143-6 PG 2 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA TN232 UT WOS:A1996TN23200021 PM 8540467 ER PT J AU Tanasijevic, MJ Winkelman, JW Wybenga, DR Richardson, DK Greene, MF AF Tanasijevic, MJ Winkelman, JW Wybenga, DR Richardson, DK Greene, MF TI Prediction of fetal lung maturity in infants of diabetic mothers using the FLM S/A and disaturated phosphatidylcholine tests SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE fetal lung maturity; surfactant albumin ratio; phosphatidylcholine; diabetes mellitus ID RESPIRATORY-DISTRESS SYNDROME; LECITHIN-SPHINGOMYELIN RATIO; AMNIOTIC-FLUID; PREGNANCIES; MELLITUS; MATURATION; ASSAY AB The authors evaluated the performance of the amniotic fluid surfactant to albumin ratio (FLM S/A), and disaturated phosphatidylcholine (DSPC) tests in assessing fetal lung maturity in infants of mothers with insulin-dependent diabetes mellitus antedating pregnancy, The distribution of the study population (n = 180) by class of diabetes was class B (27%); class C (28%); class D (29%); classes F, FR and T (8%); and class R patients (8%), The diagnosis of respiratory distress syndrome (RDS) was the standard for evaluating the performance of FLM S/A and DSPC, The mean estimated gestational age was 37.4 weeks. Three infants (1.7%) were diagnosed with RDS. All three were delivered before 36 weeks. FLM S/A at the cut-off for ''maturity'' of greater than or equal to 70 mg/g, had a sensitivity of 66.6%, specificity of 94.9%, positive predictive value (PPV) of 18.2%, and negative predictive value (NPV) of 99.4%, DSPC at the cut-off for ''maturity'' of 1,000 mu g/dL, had identical sensitivity and NPV, but lower specificity (89.2%) and PPV (9.5%) than FLM S/A. Both tests mispredicted maturity in the same case of RDS, The false ''mature'' rate of FLM S/A was 0.6% (95% confidence interval 0.0%-3.2%). The FLM S/A result of greater than or equal to 70 mg/g, obtained at or near-term, is a reliable predictor of the absence of RDS in infants of mothers with diabetes mellitus antedating pregnancy. C1 BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,JOINT PROGRAM NEONATOL,BOSTON,MA 02115. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT OBSTET & GYNECOL,BOSTON,MA. NR 26 TC 8 Z9 9 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD JAN PY 1996 VL 105 IS 1 BP 17 EP 22 PG 6 WC Pathology SC Pathology GA TQ290 UT WOS:A1996TQ29000005 PM 8561082 ER PT J AU Ferry, JA Yang, WI Zukerberg, LR Wotherspoon, AC Arnold, A Harris, NL AF Ferry, JA Yang, WI Zukerberg, LR Wotherspoon, AC Arnold, A Harris, NL TI CD5+ extranodal marginal zone B-cell (MALT) lymphoma - A low grade neoplasm with a propensity for bone marrow involvement and relapse SO AMERICAN JOURNAL OF CLINICAL PATHOLOGY LA English DT Article DE lymphoma; marginal zone; MALT; orbit; CD5; bcl-1; cyclin D1; cytogenetics ID PERIPHERAL-BLOOD INVOLVEMENT; IMMUNOPHENOTYPIC SPECTRUM; CLINICAL PRESENTATION; CENTROCYTIC LYMPHOMA; TISSUE MALT; FEATURES; EXPRESSION; DISTINCT; LEUKEMIA AB Three cases of extranodal marginal zone B-cell lymphoma (low grade B-cell lymphoma of mucosa-associated lymphoid tissue [MALT] type) in which the neoplastic B cells expressed the CD5 antigen are reported. The patients included 2 men and 1 woman, aged 44, 62, and 77 years. In all three cases, the histologic features were typical of marginal zone/MALT lymphoma, with reactive follicles, marginal zone (centrocyte-like) cells, and plasma cells, Pseudofollicles, prolymphocytes, and paraimmunoblasts were absent. In all cases, lymphoma from one or more sites expressed monotypic immunoglobulin (2 IgM kappa, 1 IgM lambda), pan B cell antigens and CD5, Two of 3 cases expressed CD43; one case expressed CD23. No case showed overexpression of the bcl-1 protein, cyclin D1. Interphase cytogenetic analysis revealed trisomy 3 in one of two cases examined, The two male patients presented with lymphoma in the ocular adnexa, One of them had marrow involvement, cervical lymphadenopathy and peripheral blood involvement at presentation; 24 months later, he developed a relapse in subcutaneous tissue, The second patient had marrow involvement 3 years later, at the time of recurrence of his orbital disease. The third patient presented with lymphoma at the base of the tongue, She subsequently developed lymphoma involving the left upper eyelid and right lacrimal sac and duct, the marrow, and the nasopharynx between 63 and 95 months after initial presentation. All of these patients presented with disease involving sites in the head and neck and all had multiple relapses or recurrences with bone marrow involvement at the time of presentation (1 case) or at relapse (2 cases). The presence of CD5 may be a marker for cases of MALT lymphoma with a tendency for persistent or recurrent disease, for dissemination to the marrow and other extranodal sites, and for leukemic involvement of the peripheral blood. C1 MASSACHUSETTS GEN HOSP,JAMES HOMER WRIGHT PATHOL LABS,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. MASSACHUSETTS GEN HOSP,ENDOCRINE ONCOL LAB,BOSTON,MA. UNIV LONDON,ROYAL POSTGRAD MED SCH,HAMMERSMITH HOSP,LONDON,ENGLAND. FU NCI NIH HHS [CA55909] NR 33 TC 105 Z9 106 U1 1 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0002-9173 J9 AM J CLIN PATHOL JI Am. J. Clin. Pathol. PD JAN PY 1996 VL 105 IS 1 BP 31 EP 37 PG 7 WC Pathology SC Pathology GA TQ290 UT WOS:A1996TQ29000007 PM 8561085 ER PT J AU Milby, JB Sims, MK Khuder, S Schumacher, JE Huggins, N McLellan, AT Woody, G Haas, N AF Milby, JB Sims, MK Khuder, S Schumacher, JE Huggins, N McLellan, AT Woody, G Haas, N TI Psychiatric comorbidity: Prevalence in methadone maintenance treatment SO AMERICAN JOURNAL OF DRUG AND ALCOHOL ABUSE LA English DT Article ID OPIATE ADDICTS; NARCOTIC ADDICTS; DIAGNOSIS; DEPRESSION; DISORDERS; PSYCHOPATHOLOGY AB This study examines prevalence rates for DSM-III-R anxiety and affective disorders in three follow-up samples of opioid addicts who were treated with methadone maintenance. At least one anxiety disorder was diagnosed in 55% of the total sample. Affective disorders were found in 58%. At least one anxiety disorder coexisted with at least one affective disorder in 36% of the sample. The research demonstrates that opiate addiction in this sample is most often associated with other comorbid psychopathology. it suggests a need for thorough assessment for general psychopathology in opioid addicts entering addiction treatment, especially assessment for anxiety and affective disorders. It also suggests the need for treatment that focuses on diagnosed mental disorders in addition to drug counseling for the substance abuse disorder. C1 UNIV ALABAMA,BIRMINGHAM,AL. VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. UNIV PENN,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,SEPULVEDA,CA 91343. UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. RP Milby, JB (reprint author), VET AFFAIRS MED CTR,BIRMINGHAM,AL 35233, USA. FU NIDA NIH HHS [1 R01 DA 05502-01A1] NR 19 TC 55 Z9 57 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0095-2990 J9 AM J DRUG ALCOHOL AB JI Am. J. Drug Alcohol Abuse PY 1996 VL 22 IS 1 BP 95 EP 107 DI 10.3109/00952999609001647 PG 13 WC Psychology, Clinical; Substance Abuse SC Psychology; Substance Abuse GA TX781 UT WOS:A1996TX78100006 PM 8651147 ER PT J AU Sorensen, G Lewis, B Bishop, R AF Sorensen, G Lewis, B Bishop, R TI Gender, job factors, and coronary heart disease risk SO AMERICAN JOURNAL OF HEALTH BEHAVIOR LA English DT Article ID CARDIOVASCULAR-DISEASE; MYOCARDIAL-INFARCTION; WORKING POPULATION; BLOOD-PRESSURE; RANDOM SAMPLE; STRAIN; SUPPORT; HEALTH; STRESS; WOMEN AB This study compares the relationship of job experiences, including psychological, job demands and job-decision latitude, to risk factors for coronary heart disease, including serum cholesterol and diastolic blood pressure, in men and women. Cross-sectional data were collected from 360 randomly selected employed subscribers to a group model health maintenance organization in central Massachusetts. Multiple regression analysis was used to examine the relationship of job-decision latitude, total psychological stressors, physical exertion, and work hours to two dependent variables, diastolic blood pressure and total serum cholesterol, controlling for gender, age, and education;interactions between gender and each job factor were also tested. For both men and women, high job-decision latitude and fewer work hours were associated with lower levels of risk factors for coronary heart disease. One gender difference was noted: Physical exertion was related to higher blood pressure levels in women, but to lower levels in men. Several reasons for this observed difference are discussed. This study underlines the importance of gob factors, especially job-decision latitude, for risk of coronary heart disease among both men and women. C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. ST VINCENTS HOSP,DEPT CARDIOL,WORCESTER,MA. RP Sorensen, G (reprint author), DANA FARBER CANC INST,44 BINNEY ST,BOSTON,MA 02115, USA. NR 36 TC 1 Z9 1 U1 1 U2 4 PU PNG PUBLICATIONS PI STAR CITY PA PO BOX 4593, STAR CITY, WV 26504-4593 SN 0147-0353 J9 AM J HEALTH BEHAV JI Am. J. Health Behav. PD JAN-FEB PY 1996 VL 20 IS 1 BP 3 EP 13 PG 11 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA TY462 UT WOS:A1996TY46200001 ER PT J AU Carter, TB Garris, AG Ullian, ME AF Carter, TB Garris, AG Ullian, ME TI Rusty peritoneal dialysis fluid after intravenous administration of iron dextran SO AMERICAN JOURNAL OF KIDNEY DISEASES LA English DT Article DE iron dextran; rifampicin; peritoneal dialysis; peritonitis ID DEFEROXAMINE AB Rusty-colored peritoneal dialysate fluid was observed after intravenous administration of iron dextran to a patient with peritonitis being treated with vancomycin and rifampicin. The discoloration gradually cleared over a 24-hour period. Analysis of the fluid demonstrated that the discoloration could not be explained by the presence of erythrocytes or free hemoglobin, Iron (52 mu g/dL) was detected in the fluid and decreased to undetectable levels as the discoloration cleared, Addition of iron dextran to an unused bag of peritoneal dialysis fluid to achieve an iron concentration of 52 mu g/dL resulted in no discoloration, Addition of rifampicin at a clinically relevant serum concentration (10 mu g/mL) to a different unused bag caused a light orange discoloration, Addition of iron dextran and rifampicin simultaneously in the concentrations mentioned to an unused bag caused a rusty discoloration almost as dark as that observed in our patient, We postulate, therefore, that a combination of iron and rifampicin caused the marked discoloration of our patient's peritoneal effluent. C1 MED UNIV S CAROLINA,DIV NEPHROL,CHARLESTON,SC 29425. RALPH H JOHNSON VET ADM MED CTR,CHARLESTON,SC. NR 14 TC 9 Z9 9 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0272-6386 J9 AM J KIDNEY DIS JI Am. J. Kidney Dis. PD JAN PY 1996 VL 27 IS 1 BP 147 EP 150 DI 10.1016/S0272-6386(96)90044-X PG 4 WC Urology & Nephrology SC Urology & Nephrology GA TN799 UT WOS:A1996TN79900020 PM 8546131 ER PT J AU Koziel, MJ AF Koziel, MJ TI Immunology of viral hepatitis SO AMERICAN JOURNAL OF MEDICINE LA English DT Article AB So far, five major forms of viral hepatitis, hepatitis A, B, C, D, and E, have been identified. There appears to be at least one other form of enterically transmitted and one other parenterally transmitted hepatotropic virus, but characterization of these viruses is still preliminary. The five hepatotropic viruses have unique structures, yet all the share the property of inducing hepatocellular damage, whether through direct cytotoxicity or through induction of immune mechanisms that lead to hepatocellular necrosis. Advances in molecular biology in the past decade have enabled researchers to understand much about the structure, mechanisms of replication, and viral life cycle of each of these viruses, and successful vaccines have been developed for hepatitis A and B. However, many problems remain unsolved, including which immune system factors are important defenses against these viral infections, which components of the immune system are necessary for a successful vaccine, and what allows some viruses, such as hepatitis B and hepatitis C virus, to become persistent and lead to chronic liver disease. RP Koziel, MJ (reprint author), MASSACHUSETTS GEN HOSP,INFECT DIS UNIT,GRAY 504,32 FRUIT ST,BOSTON,MA 02114, USA. NR 6 TC 48 Z9 53 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0002-9343 J9 AM J MED JI Am. J. Med. PD JAN PY 1996 VL 100 IS 1 BP 98 EP 109 DI 10.1016/S0002-9343(96)90018-2 PG 12 WC Medicine, General & Internal SC General & Internal Medicine GA TT033 UT WOS:A1996TT03300018 PM 8579095 ER PT J AU Cheney, ML Megerian, CA Brown, MT McKenna, MJ Nadol, JB AF Cheney, ML Megerian, CA Brown, MT McKenna, MJ Nadol, JB TI The use of the temporoparietal fascial flap in temporal bone reconstruction SO AMERICAN JOURNAL OF OTOLOGY LA English DT Article DE temporoparietal fascial flap; temporal bone; reconstruction; mastoiditis; osteoradionecrosis of temporal bone ID NECK RECONSTRUCTION; EAR RECONSTRUCTION; HEAD AB After routine canal wall down mastoidectomy, local muscle flaps with and without bone pate, cartilage and fascia are the standard techniques available to otologists wishing to obliterate the mastoid and reconstruct the external auditory canal. Reconstructive options for temporal bone defects after extirpative surgery for cancer, osteoradionecrosis, and revision surgery for chronic granulomatous otitis media, however, are few. Although the neighboring temporoparietal fascia flap (TPFF), based on the superficial temporal vessels, has been frequently employed for auricular reconstruction, its versatility in temporal bone reconstruction has not been widely explored. The TPFF has recently been employed at our institution in 11 patients who presented with a variety of reconstructive problems, including defects after temporal bone resection, surgery for malignant otitis externa, and revision mastoid surgery. Follow-up in these patients ranged from 1 to 43 months (average 18.4 months) and surgical objectives of achieving a dry mastoid bowl, fully epithelialized canal, and/or reduction of mastoid cavity volume was attained in 100% of cases. The TPFF offers many advantages to the otologic surgeon when faced with reconstruction dilemmas that center around a poorly vascularized mastoid cavity and temporal bone. The TPFF is a reliable source of local well-vascularized tissue that is extremely pliable and facilitates both hearing and nonhearing preservation temporal bone reconstruction. C1 HARVARD UNIV,SCH MED,DEPT OTOL & LARYNGOL,BOSTON,MA. RP Cheney, ML (reprint author), MASSACHUSETTS EYE & EAR INFIRM,DEPT OTOLARYNGOL,243 CHARLES ST,BOSTON,MA 02114, USA. NR 16 TC 23 Z9 23 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0192-9763 J9 AM J OTOL JI Am. J. Otol. PD JAN PY 1996 VL 17 IS 1 BP 137 EP 142 PG 6 WC Otorhinolaryngology SC Otorhinolaryngology GA TY970 UT WOS:A1996TY97000028 PM 8694118 ER PT J AU BeDell, KK Scremin, AME Perell, KL Kunkel, CF AF BeDell, KK Scremin, AME Perell, KL Kunkel, CF TI Effects of functional electrical stimulation-induced lower extremity cycling on bone density of spinal cord-injured patients SO AMERICAN JOURNAL OF PHYSICAL MEDICINE & REHABILITATION LA English DT Article DE osteoporosis; spinal cord-injured; functional electrical stimulation; exercise ID DUAL-PHOTON ABSORPTIOMETRY; MINERAL DENSITY; OSTEOPOROSIS; WEIGHT; AGE; IMMOBILIZATION; HYPERCALCIURIA; MASS; HIP AB Spinal cord-injured (SCI) patients are at increased risk for fractures secondary to neurogenic osteoporosis. Earlier research claimed physical conditioning resulted in a decreased incidence or reversal of neurogenic osteoporosis. This study evaluated the effects of functional electrical stimulation-induced lower extremity cycling (FESILEC) on the bone densities of SCI patients using dual-energy x-ray absorptiometry (DEXA). The study consisted of 12 healthy male SCI patients, aged 23 to 46 (x +/- SD, 34 +/- 6) yr. The patients were post-traumatic, complete, spastic SCI; time postinjury ranged from 2 to 19 (9.7 +/- 5.1) yr. Patients participated in a three-phase training program. Phase 1 consisted of quadriceps strengthening. Phase 2 consisted of progressive sequential stimulation of quadriceps, hamstrings, and gluteal muscles, achieving a rhythmical pedaling motion on the REGYS I ergometer. Phase 3a consisted of 30-min FESILEC sessions. DEXAs were done at baseline and at completion of Phase 3a and Phase 3b. Bone densities were done of the lumbar spine levels 2-4 (L2-4), bilateral trochanters (T), Ward's triangles (WT), and femoral necks (FN). Baseline bone density indicated no difference between L2-4 of ambulatory males and SCI males. Baseline values obtained for T, WT, and FN were, respectively, 71, 82, and 79% of ambulatory values, Results after completion of the Phase 3a training program indicated no statistically significant difference compared with baseline values. There was, however, a positive trend in the lumbar spine post-Phase 3a (L2-4, P = 0.056). Eight patients continued the exercise program, using a combination of upper and lower extremity cycling (Phase 3b) for a longer period of time (25 +/- 9 wk). DEXAs done after Phase 3b indicated no change relative to baseline data or data post-Phase 3a. In conclusion, although FESILEC did not significantly increase bone density in the hip parameters of chronic SCI patients, a positive trend was observed in the lumbar spine. Further research with acute intervention, such as FESILEC during the first few months post-SCI, is warranted to further evaluate a treatment regimen to prevent or reduce neurogenic osteopenia. C1 W LOS ANGELES VET AFFAIRS MED CTR,PHYS MED & REHABIL SERV,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,DEPT MED,DIV PHYS MED & REHABIL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT PHYSIOL SCI,LOS ANGELES,CA 90024. UNIV NEW MEXICO,ALBUQUERQUE DEPT VET AFFAIRS MED CTR,PHYS MED & REHABIL SERV,ALBUQUERQUE,NM 87131. UNIV NEW MEXICO,DEPT ORTHOPED,ALBUQUERQUE,NM 87131. NR 33 TC 70 Z9 75 U1 2 U2 6 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0894-9115 J9 AM J PHYS MED REHAB JI Am. J. Phys. Med. Rehabil. PD JAN-FEB PY 1996 VL 75 IS 1 BP 29 EP 34 DI 10.1097/00002060-199601000-00008 PG 6 WC Rehabilitation; Sport Sciences SC Rehabilitation; Sport Sciences GA TY971 UT WOS:A1996TY97100007 PM 8645435 ER PT J AU Eakes, AT Hymer, TK Rosenthal, MJ Moss, J Katz, MS AF Eakes, AT Hymer, TK Rosenthal, MJ Moss, J Katz, MS TI Alterations of adenylyl cyclase-linked G proteins in rat liver during aging SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE beta-adrenergic receptor; cholera toxin; pertussis toxin; adenosine 5'-diphosphate ribosylation factor ID BETA-ADRENERGIC-RECEPTOR; ADP-RIBOSYLATION; HEPATOCYTES; BINDING; GTP; IDENTIFICATION; GLYCOGENOLYSIS; SUBUNITS; ENZYME AB beta-Adrenergic stimulation of adenylyl cyclase in rat liver increases during aging. We examined whether this increase is related to alterations in the stimulatory and inhibitory G proteins (G(s) and G(i)) linked to adenylyl cyclase. Levels of immunoreactive alpha- and beta-subunits of G(s) and G(i) in liver plasma membranes from 6-, 12-, 18-, and 24-mo-old rats were unchanged with age, as was pertussis toxin-catalyzed [P-32]ADP ribosylation of G(i) alpha. Cholera toxin-catalyzed [P-32]ADP ribosylation of G(s) alpha and G(s) bioactivity, assessed as reconstitution of adenylyl cyclase activity in S49 cyc(-) cell membranes, increased two- to threefold between 6 and 12-18 mo, and declined by 24 mo. Recombinant ADP ribosylation factor (ARF) enhanced cholera toxin labeling of G(s) alpha at all ages, yet abolished the increase in toxin labeling at 12-18 mo. Auto-ADP ribosylation of the cholera toxin A(1) peptide also increased transiently with age. Alteration of G(s) alpha, as reflected by increased cholera toxin labeling and G(s) bioactivity, may be involved in the regulation of beta-adrenergic-responsive adenylyl cyclase in rat liver during aging. Moreover, changes in endogenous ARF levels could contribute to age differences in cholera toxin labeling of G(s) alpha. C1 UNIV TEXAS, GERIATR RES EDUC & CLIN CTR 182, AUDIE L MURPHY MEM VET HOSP,DEPT MED, HLTH SCI CTR, SAN ANTONIO, TX 78284 USA. UNIV CALIF LOS ANGELES, SCH MED, SAN FERNANDO VALLEY PROGRAM, SEPULVEDA, CA 91343 USA. VET AFFAIRS MED CTR, CTR GERIATR RES EDUC & CLIN, SEPULVEDA, CA 91343 USA. NHLBI, NIH, PULM CRIT CARE MED BRANCH, BETHESDA, MD 20892 USA. NR 32 TC 5 Z9 5 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JAN PY 1996 VL 270 IS 1 BP E126 EP E132 PG 7 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA TT393 UT WOS:A1996TT39300019 PM 8772484 ER PT J AU Lloyd, KCK Grandt, D Aurang, K Eysselein, VE Schimiczek, M Reeve, JR AF Lloyd, KCK Grandt, D Aurang, K Eysselein, VE Schimiczek, M Reeve, JR TI Inhibitory effect of PYY on vagally stimulated acid secretion is mediated predominantly by Y-1 receptors SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE peptide tyrosine tyrosine; gastric acid secretion; insulin-induced hypoglycemia; cephalic phase ID PEPTIDE-YY PYY; NEUROPEPTIDE-Y; STRUCTURAL CHARACTERIZATION; SOMATOSTATIN RELEASE; RAT-BRAIN; RABBIT; SOLUBILIZATION; HORMONE; DOG AB Two molecular forms of peptide YY (PW), PYY-(1-36) and PYY-(3-36), are abundant in rabbit intestine and blood. We have previously shown that PYY-(1-36) (PW I) activates equipotently Y-1 and Y-2 receptors and PYY-(3-36) (PYY II) is a highly selective agonist for Y-2 receptors. In the present study, we examined the effect of exogenous infusion of PYY on vagally stimulated gastric acid secretion in awake rabbits with chronic gastric fistula. To determine the specific PW receptor(s) that mediates this effect, we used a highly selective Y-1 agonist, Pro(34)-PYY, a synthetic PYY, and a Y-2-selective agonist, PYY II. Vagal stimulation of acid secretion was elicited by an intravenous bolus injection of insulin (0.125 U/kg) 30 min after beginning a 180-min intravenous infusion of either PW I, PYY II, or [Pro(34)]-PYY after a 50 mu g/kg iv bolus of atropine followed immediately by a 500 mu g/kg sc injection. During infusion of 200 pmol . kg(-1). h(-1) PW I, acid output was significantly inhibited to 45 +/- 13% of maximum acid output 60 min after injection of insulin. Similarly, acid output during infusion of 200 pmol . kg(-1). h(-1) [Pro(34)]-PYY was significantly inhibited to 52 +/- 12% of maximum. In contrast, acid output during infusion of 200 pmol . kg(-1). h(-1) of PW II was not significantly inhibited (101 +/- 18% of maximum). Infusion of double the dose (400 pmol . kg(-1). h(-1)) of PYY II resulted in acid inhibition (51 +/- 15% of maximum), whereas infusion of the same dose did not significantly enhance acid inhibition by infusion of either PW I or [Pro(34)]-PYY (28 +/- 11 and 42 +/- 15% of maximum). These results indicate that PYY, acting predominantly at Y-1 receptors, is a potent inhibitor of vagally stimulated acid secretion in adult rabbits. C1 W LOS ANGELES VET AFFAIRS MED CTR, DEPT VET AFFAIRS, RES & MED SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED,DEPT MED,CTR ULCER RES & EDUC, DIGEST DIS CORE CTR, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, MED CTR, LOS ANGELES, CA 90073 USA. UNIV KLINIKUM ESSEN, ZENTRUM INNERE MED, D-45122 ESSEN, GERMANY. FU NIDDK NIH HHS [DK-41301] NR 36 TC 13 Z9 13 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD JAN PY 1996 VL 270 IS 1 BP G123 EP G127 PG 5 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA TT394 UT WOS:A1996TT39400016 PM 8772509 ER PT J AU Nishizaki, Y Guth, PH Sternini, C Kaunitz, JD AF Nishizaki, Y Guth, PH Sternini, C Kaunitz, JD TI Impairment of the gastric hyperemic response to luminal acid in cirrhotic rats SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE carbon tetrachloride; stomach; mucosal blood flow; pentagastrin; calcitonin gene-related peptide; afferent nerves; ethanol ID MUCOSAL BLOOD-FLOW; PORTAL HYPERTENSIVE RATS; INTRACELLULAR PH; REFLECTANCE SPECTROPHOTOMETRY; CONGESTIVE GASTROPATHY; LIVER-CIRRHOSIS; SECRETION; NEURONS; CELL; IMMUNOREACTIVITY AB Liver cirrhosis impairs gastric mucosal resistance to luminal acid in humans and in animal models. Because we have previously shown that pentagastrin enhances defensive as well as aggressive factors implicated in mucosal injury, we examined the hypothesis that the pentagastrin-mediated enhancement of mucosal defense mechanisms may be impaired in cirrhotic rats. Increased acid backdiffusion and susceptibility to gross mucosal injury, associated with an elimination of the hyperemic response to gastric barrier disruption, was observed in cirrhotic rats. In in vivo microscopic studies in anesthetized rats, cirrhosis had no effect on pentagastrin-associated enhancement of mucus gel thickness or baseline gastric mucosal blood flow although baseline mucus gel thickness was decreased. Cirrhosis did, however, abolish the luminal acid-related hyperemic response to pentagastrin, which was associated with impaired intracellular pH homeostasis:during acid superfusion. Cirrhosis did not alter submucosal calcitonin gene-related peptide immunoreactive nerves. We conclude that acid backdiffusion and pentagastrin-associated hyperemic responses are important mucosal defensive factors that are specifically impaired by cirrhosis. C1 W LOS ANGELES VET AFFAIRS MED CTR, MED SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, GASTROENTER BIOL CTR, CTR ULCER RES & EDUC, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT ANAT, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90024 USA. NR 34 TC 6 Z9 6 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0193-1857 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD JAN PY 1996 VL 270 IS 1 BP G71 EP G78 PG 8 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA TT394 UT WOS:A1996TT39400010 PM 8772503 ER PT J AU Berk, DA Swartz, MA Leu, AJ Jain, RK AF Berk, DA Swartz, MA Leu, AJ Jain, RK TI Transport in lymphatic capillaries .2. Microscopic velocity measurement with fluorescence photobleaching SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE fluorescence recovery after photobleaching; fluorescence microlymphography; skin lymphatics; mouse; dextran; microsphere; spatial Fourier transform; image processing ID SPATIAL FOURIER-ANALYSIS; DIFFUSION; FLOW; RECOVERY; PRESSURE; FLUID AB Despite its relevance to the physiology of lymph formation and propulsion, the instantaneous flow velocity in single lymphatic capillaries has not been measured to date. The method of fluorescence recovery after photobleaching (FRAP) was adapted for this purpose and used to characterize flow in the lymphatic capillaries in tail skin of anesthetized mice during a constant-pressure intradermal injection of fluorescein isothiocyanate-dextran (mol wt 2 x 10(6)). The median lymph flow velocity was 4.7 mu m/s, and the velocity magnitude ranged from 0 to 29 mu m/s. The direction of flow was generally proximal, but stasis and backflow toward the site of injection was also detected. Evidence for oscillatory flow was detected in some FRAP experiments, and in separate experiments a periodicity of similar to 120 min(-1), directly correlated to respiration frequency, was measured by tracking the motion of fluorescent latex microspheres (1 mu m diam) introduced into the lymphatic capillary network. The velocity magnitude showed a correlation with duration of infusion but not with distance from injection site. It is speculated that the temporal decay of mean velocity magnitude could be related to the relaxation of local pressure gradients as partially collapsed vessels expand during the infusion. C1 MASSACHUSETTS GEN HOSP, DEPT RADIAT ONCOL, EDWIN L STEELE LAB, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. MIT, DEPT CHEM ENGN, CAMBRIDGE, MA 02139 USA. RI Swartz, Melody/B-7633-2009; Berk, David/A-4863-2012; Swartz, Melody/F-9563-2011 OI Berk, David/0000-0002-3855-6886; FU NCI NIH HHS [CA-56591, CA-59255] NR 18 TC 69 Z9 69 U1 2 U2 13 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD JAN PY 1996 VL 270 IS 1 BP H330 EP H337 PG 8 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA TV391 UT WOS:A1996TV39100043 PM 8769769 ER PT J AU Swartz, MA Berk, DA Jain, RK AF Swartz, MA Berk, DA Jain, RK TI Transport in lymphatic capillaries .1. Macroscopic measurements using residence time distribution theory SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY LA English DT Article DE microcirculation; fluorescence microlymphography; lymphatic uptake; lymphatic flow; dextran ID INTERSTITIAL FLUID; RAT TAIL; VOLUME; FLOW AB We present a novel integrative method for characterizing transport in the lymphatic capillaries in the tail of the anesthetized mouse, which is both sensitive and reproducible for quantifying uptake and flow. Interstitially injected, fluorescently labeled macromolecules were used to visualize and quantify these processes. Residence time distribution (RTD) theory was employed to measure net flow velocity in the lymphatic network as well as to provide a relative measure of lymphatic uptake of macromolecules from the interstitium. The effects of particle size and injection pressure were determined. The uptake rate was found to be independent of particle size in the range of a 6- to 18-nm radius; beyond this size, the interstitial matrix seemed to pose a greater barrier. A comparison of 10 vs. 40 cmH(2)O injection pressure showed a significant influence on the relative uptake rate but not on the net velocity within the network (3.3 +/- 0.8 vs. 3.8 +/- 1.0 mu m/s). This suggested the presence of a systemic driving force for baseline lymph propulsion that is independent of the local pressure gradients driving the uptake. This model can be used to examine various aspects of transport physiology of the initial lymphatics. C1 MASSACHUSETTS GEN HOSP, DEPT RADIAT ONCOL, EDWIN L STEELE LAB, BOSTON, MA 02114 USA. MIT, DEPT CHEM ENGN, CAMBRIDGE, MA 02139 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. RI Swartz, Melody/B-7633-2009; Berk, David/A-4863-2012; Swartz, Melody/F-9563-2011 OI Berk, David/0000-0002-3855-6886; FU NCI NIH HHS [CA-56591, CA-59255] NR 28 TC 98 Z9 102 U1 0 U2 9 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0363-6135 J9 AM J PHYSIOL-HEART C JI Am. J. Physiol.-Heart Circul. Physiol. PD JAN PY 1996 VL 270 IS 1 BP H324 EP H329 PG 6 WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Physiology GA TV391 UT WOS:A1996TV39100042 PM 8769768 ER PT J AU Abcouwer, SF Lukaszewicz, GC Souba, WW AF Abcouwer, SF Lukaszewicz, GC Souba, WW TI Glucocorticoids regulate glutamine synthetase expression in lung epithelial cells SO AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY LA English DT Article DE L2 cells; sepsis; dexamethasone; messenger ribonucleic acid stability; cycloheximide ID PROTEIN-SYNTHESIS INHIBITORS; CULTURED 3T3-L1 ADIPOCYTES; SKELETAL-MUSCLE CELLS; MESSENGER-RNA; C-FOS; FUNCTIONAL-CHARACTERIZATION; ALANINE METABOLISM; GENE-TRANSCRIPTION; DEXAMETHASONE; INDUCTION AB During septic states efflux of glutamine from the lung increases, a response sustained by an increase in glutamine synthetase (GS) activity. We have used a cell culture model employing a rat epithelial cell line of pulmonary origin (L2 cells) to study the effect of several hormones and cytokines which mediate the septic shock response on GS expression. We found that GS mRNA and GS protein contents increased rapidly and severalfold in response to physiologically relevant levels of the synthetic glucocorticoid dexamethasone (Dex). In contrast, GS expression was not markedly induced by Escherichia coli lipopolysaccharide (LPS), cytokines, activated complement C5a, or prostaglandins. Dex did not alter the kinetics of GS mRNA decay in the presence of actinomycin D. The increase in GS mRNA in response to Dex was completely blocked by RU-38486 and by actinomycin D, but not by cycloheximide (CHX). CHX together with Dex caused a superinduction of GS mRNA. GS mRNA decay kinetics suggested that this superinduction is at least in part caused by an approximately twofold increase in GS mRNA half-life caused by CHX. In addition, actinomycin D was found to increase GS mRNA half-life by similar to 50%. Actinomycin D plus CHX acted synergistically to cause a profound inhibition of GS mRNA decay. Our results are consistent with regulation of lung GS expression via a direct glucocorticoid receptor-mediated response. In addition, GS mRNA decay in L2 cells seems to be regulated by two independent mechanisms, one which is sensitive to CHX and one which is sensitive to actinomycin D. C1 HARVARD UNIV, MASSACHUSETTS GEN HOSP,SCH MED,DEPT MED, DIV SURG ONCOL, BOSTON, MA 02114 USA. FU NHLBI NIH HHS [R01 HL-44986] NR 47 TC 28 Z9 28 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 1040-0605 J9 AM J PHYSIOL-LUNG C JI Am. J. Physiol.-Lung Cell. Mol. Physiol. PD JAN PY 1996 VL 270 IS 1 BP L141 EP L151 PG 11 WC Physiology; Respiratory System SC Physiology; Respiratory System GA TT387 UT WOS:A1996TT38700016 PM 8772537 ER PT J AU Lee, KC Brown, D Urena, P Ardaillou, N Ardaillou, R Deeds, J Segre, GV AF Lee, KC Brown, D Urena, P Ardaillou, N Ardaillou, R Deeds, J Segre, GV TI Localization of parathyroid hormone parathyroid hormone-related peptide receptor mRNA in kidney SO AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY LA English DT Article DE parathyroid hormone parathyroid hormone-related peptide receptor in podocytes; multiple parathyroid hormone parathyroid hormone-related peptide receptor transcripts; in situ hybridization ID GLOMERULAR ULTRAFILTRATION; RAT; REABSORPTION; CALCIUM; TUBULE; CELLS; PTH; MECHANISMS; COLLAGEN; NEPHRON AB In kidney, parathyroid hormone (PTH) exerts differential distal effects along the nephron. To define cells expressing receptors for PTH in kidney, we localized PTH/PTH-related peptide (PTH/PTHrP) receptor mRNA in rat kidney by in situ hybridization. PTH/PTHrP receptor mRNA is localized to glomerular podocytes, convoluted and straight proximal tubules, the cortical portion of thick ascending limbs, and distal convoluted tubules, but was not detected in the thin limb of Henle's loop or in collecting ducts. Northern blot analysis showed that cultured human glomerular podocytes express a unique 4.0-kb PTH/PTHrP receptor transcript but do not express detectable levels of the common similar to 2.4-kb transcript found in whole kidney and in many other tissues. Whereas the tubular localization of PTH/PTHrP receptor mRNA coincides well with previously known sites of PTH action, the intense expression and the unique size of the PTH/PTHrP receptor transcript in glomerular podocytes suggest that PTH and/or PTHrP may play a role(s) in glomerular function. C1 MASSACHUSETTS GEN HOSP, RENAL UNIT, DEPT MED, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, DEPT MED, BOSTON, MA 02114 USA. HOP NECKER ENFANTS MALAD, INSERM 90, PARIS, FRANCE. HOP TENON, INSERM 64, PARIS, FRANCE. RP Lee, KC (reprint author), MASSACHUSETTS GEN HOSP, ENDOCRINE UNIT, FRUIT ST, BOSTON, MA 02114 USA. FU NIDDK NIH HHS [DK-11794, DK-42956, DK-47237] NR 29 TC 67 Z9 67 U1 0 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6127 J9 AM J PHYSIOL-RENAL JI Am. J. Physiol.-Renal Fluid Electrolyte Physiol. PD JAN PY 1996 VL 270 IS 1 BP F186 EP F191 PG 6 WC Physiology; Urology & Nephrology SC Physiology; Urology & Nephrology GA TT558 UT WOS:A1996TT55800022 PM 8769838 ER PT J AU Paul, RV Saxenhofer, H Wackym, PS Halushka, PV AF Paul, RV Saxenhofer, H Wackym, PS Halushka, PV TI Stimulation of rat mesangial cell thromboxane A(2) receptors inhibits particulate but not soluble guanylyl cyclase SO AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY LA English DT Article DE guanosine 3',5'-cyclic monophosphate; radioligand; natriuretic peptides; nitroprusside ID ATRIAL NATRIURETIC FACTOR; VASCULAR SMOOTH-MUSCLE; MURINE LUPUS NEPHRITIS; CGMP ACCUMULATION; PLATELET; ACTIVATION; INJURY AB Thromboxane A(2) (TxA(2)) participates in the pathogenesis of clinical and experimental glomerular disease. We performed radioligand binding and functional studies of TxA(2) receptors in rat mesangial cells. Competitive inhibition of specific binding of the TxA(2) analogue [I-125]BOP by unlabeled antagonists in intact cells or membranes was observed, with a rank order potency of SQ-29548 (half-maximal inhibitory concentration = 3.4 nM > L-657925 (21 nM)> GR-32191 (200 nM) > L-657926 (1,300 nM). The potency of agonists was I-BOP (0.43 nM) > ONO-11113 (6.7 nM) > U-46619 (80 nM). U-46619 and unlabeled I-BOP inhibited the rate of net guanosine 3',5'-cyclic monophosphate accumulation in cells exposed to atrial natriuretic peptide (ANP) but not the nitric oxide donor nitroprusside. Membranes from cells exposed to I-BOP for 10 min exhibited a 38% decrease in ANP-responsive guanylyl cyclase activity. U-46619 blocked the inhibitory effect of ANP on serum-stimulated [H-3]thymidine incorporation but not that of nitroprusside. In summary, we describe a novel effect mediated by a mesangial cell TxA(2) receptor, i.e., inhibition of ANP signaling. C1 MED UNIV S CAROLINA, DEPT MED, CHARLESTON, SC 29425 USA. MED UNIV S CAROLINA, DEPT CELLULAR & MOLEC PHARMACOL, CHARLESTON, SC 29425 USA. MED UNIV S CAROLINA, DEPT EXPTL THERAPEUT, CHARLESTON, SC 29425 USA. RP Paul, RV (reprint author), MED UNIV S CAROLINA, DIV NEPHROL, RALPH H JOHNSON DEPT VET AFFAIRS MED CTR, 171 ASHLEY AVE, CHARLESTON, SC 29425 USA. FU NHLBI NIH HHS [HL-36838] NR 36 TC 3 Z9 3 U1 0 U2 0 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0363-6127 J9 AM J PHYSIOL-RENAL JI Am. J. Physiol.-Renal Fluid Electrolyte Physiol. PD JAN PY 1996 VL 270 IS 1 BP F31 EP F38 PG 8 WC Physiology; Urology & Nephrology SC Physiology; Urology & Nephrology GA TT558 UT WOS:A1996TT55800004 PM 8769820 ER PT J AU Lee, JT Miller, CA McDonald, CT Allman, JM AF Lee, JT Miller, CA McDonald, CT Allman, JM TI Xanthogranuloma of the choroid plexus in the fat-tailed dwarf lemur (Cheirogaleus medius) SO AMERICAN JOURNAL OF PRIMATOLOGY LA English DT Article DE brain tumor; lipids; diet; captivity ID LESIONS AB This report documents the death of two fat-tailed dwarf lemurs (Cheirogaleus medius) maintained over 6 years each in our laboratory. Postmortem studies revealed xanthogranuloma of the choroid plexus, a mass replete with stored lipids, including cholesterol crystals. Six months prior to their deaths, both animals developed a peculiar head tilt and signs suggestive of neurological dysfunction. At autopsy, each had masses projecting into the lateral and IVth ventricles and an associated obstructive hydrocephalus. Cryostat sections of the brains of both lemurs showed histological features consistent with xanthogranuloma of the choroid plexus, a histologically benign and usually asymptomatic lesion found in up to 7% of human autopsies. This case is of special interest because of the unique feeding strategies in the fat-tailed dwarf lemur. Since C. medius remains in torpor for 6 months out of the year during the time of food scarcity in the forests of Madagascar, the animal must accumulate large reserves of fat during its active period. In the laboratory, however, dwarf lemurs do not normally go into torpor, and the accumulated fat is not used. The finding of this tumor, therefore, suggests that the combination of a captive high-fat diet and the unusual fat-storage mechanisms utilized by C. medius contributed to the buildup of lipids and might be etiologically related to the development of these lesions. (C) 1996 Wiley-Liss, Inc. C1 MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114. UNIV SO CALIF,SCH MED,DEPT PATHOL,LOS ANGELES,CA 90033. RP Lee, JT (reprint author), CALTECH 21676,DIV BIOL,PASADENA,CA 91125, USA. NR 22 TC 8 Z9 8 U1 1 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0275-2565 J9 AM J PRIMATOL JI Am. J. Primatol. PY 1996 VL 38 IS 4 BP 349 EP 355 DI 10.1002/(SICI)1098-2345(1996)38:4<349::AID-AJP5>3.0.CO;2-Z PG 7 WC Zoology SC Zoology GA UB137 UT WOS:A1996UB13700005 ER PT J AU Pollack, MH Kradin, R Otto, MW Worthington, J Gould, R Sabatino, SA Rosenbaum, JF AF Pollack, MH Kradin, R Otto, MW Worthington, J Gould, R Sabatino, SA Rosenbaum, JF TI Prevalence of panic in patients referred for pulmonary function testing at a major medical center SO AMERICAN JOURNAL OF PSYCHIATRY LA English DT Article; Proceedings Paper CT 8th Annual NIMH International Research Conference on Mental Health Problems in the General Health Care Sector CY SEP 05-09, 1994 CL MCLEAN, VA SP NIMH ID ANXIETY DISORDERS AB Objective: The authors examined the prevalence and correlates of panic disorder in a group of patients who were referred for pulmonary function testing. Method: Patients (N=115) were screened for the presence of panic attacks and panic disorder with a self-report questionnaire; a subgroup (N=25) received structured diagnostic assessment. Results: Of the 115 patients, 41% (N=47) reported panic attacks and 17% (N=20) met screening criteria for panic disorder. From the confirmed rate of panic disorder among the subgroup who received structured diagnostic assessment, the overall prevalence rate of panic disorder was estimated to be 11% and included six of the nine patients (67%) who had a diagnosis of chronic obstructive pulmonary disease. There were no significant differences between patients with and without panic in the severity of pulmonary function abnormalities or in the response to bronchodilators. However, patients with panic attacks were significantly more likely to report dyspnea at rest and irritable bowel symptoms and tended to report difficulty swallowing. Conclusions: This study suggests that panic disorder and subsyndromal panic are relatively common and maybe unrecognized and inadequately treated in patients who present with respiratory symptoms. C1 MASSACHUSETTS GEN HOSP,PULM UNIT,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,CRIT CARE UNIT,BOSTON,MA 02114. RP Pollack, MH (reprint author), MASSACHUSETTS GEN HOSP,ANXIETY DISORDERS PROGRAM,DEPT PSYCHIAT,ACC-815,15 PARKMAN ST,BOSTON,MA 02114, USA. NR 19 TC 43 Z9 44 U1 0 U2 0 PU AMER PSYCHIATRIC ASSOCIATION PI WASHINGTON PA 1400 K ST NW, WASHINGTON, DC 20005 SN 0002-953X J9 AM J PSYCHIAT JI Am. J. Psychiat. PD JAN PY 1996 VL 153 IS 1 BP 110 EP 113 PG 4 WC Psychiatry SC Psychiatry GA TN397 UT WOS:A1996TN39700021 PM 8540567 ER PT J AU Kacmarek, RM Ripple, R Cockrill, BA Bloch, KJ Zapol, WM Johnson, DC AF Kacmarek, RM Ripple, R Cockrill, BA Bloch, KJ Zapol, WM Johnson, DC TI Inhaled nitric oxide - A bronchodilator in mild asthmatics with methacholine-induced bronchospasm SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article ID PULMONARY-HYPERTENSION; RESPONSES; NEUROTRANSMITTER; NERVES; VALUES AB Nitric oxide (NO) reduces airway tone in the methacholine-treated guinea pig. We examined whether low levels of inhaled NO gas would relax airway smooth muscle tone in patients with mild asthma subjected to methacholine-induced bronchospasm. Thirteen adult volunteers with mild asthma inspired increasing concentrations of methacholine until their baseline forced expiratory volume in one second (FEV(1), 3.29 +/- 0.17 L, mean +/- SEM) decreased by greater than or equal to 20% (2.33 +/- 0.18 L, p < 0.01). Thereafter, they sequentially inhaled 100 parts per million (ppm) NO, 40% O-2; 40% O-2; and 100 ppm NO, 40% O-2 while spirometry was performed. Subsequent inhalation of isoproterenol returned the FEV(1) levels to baseline. Inhaling 100 ppm NO increased FEV(1) to 2.66 +/- 0.18 L (p < 0.01), and this increase was maintained after NO was discontinued. FEV(1) did not change during the second period of NO inhalation. Similar results were observed for vital capacity, but no significant effect was noted on forced expiratory flow at 25% of vital capacity or peak expiratory flow. Subjects were then divided into a responder subgroup, which showed a mean increase in FEV(1) after initial NO inhalation of 560 +/- 150 ml, and a nonresponder subgroup, which showed a mean increase in FEV(1) of 129 +/- 29 ml. Our data suggest that inhalation of nitric oxide by patients with mild asthma with methacholine-induced bronchospasm results in a minor but significant relaxation of airway tone. C1 MASSACHUSETTS GEN HOSP,DEPT ANESTHESIA,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT ALLERGY,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT PULM & CRIT CARE MED,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. RP Kacmarek, RM (reprint author), MASSACHUSETTS GEN HOSP,DEPT RESP CARE,ELLISON 401,BOSTON,MA 02114, USA. FU NHLBI NIH HHS [HL42391] NR 20 TC 84 Z9 85 U1 0 U2 3 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD JAN PY 1996 VL 153 IS 1 BP 128 EP 135 PG 8 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA TP461 UT WOS:A1996TP46100015 PM 8542105 ER PT J AU Sazon, DAD Santiago, SM Hoo, GWS Khonsary, A Brown, C Mandelkern, M Blahd, W Williams, AJ AF Sazon, DAD Santiago, SM Hoo, GWS Khonsary, A Brown, C Mandelkern, M Blahd, W Williams, AJ TI Fluorodeoxyglucose-positron emission tomography in the detection and staging of lung cancer SO AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE LA English DT Article ID SOLITARY PULMONARY NODULES; COMPUTED-TOMOGRAPHY; GLUCOSE-UTILIZATION; TUMOR; F-18; PET AB Glycolysis is increased in tumor tissues. [F-18]fluoro-2-deoxy-D-glucose (FDC) is a glucose analogue radiopharmaceutical used in positron emission tomography (PET) to trace glucose metabolism. We investigated the sensitivity and specificity of FDC-PET imaging in the diagnosis and staging of lung cancer. One hundred and seven patients who had abnormal chest roentgenograms underwent whole-body PET imaging using FDC. PET scan results were classified as positive or negative based on the presence or absence of increased FDC uptake in the lung and/or in the mediastinum. All 82 patients with lung cancer had increased FDG uptake in the lungs, whereas only 12 of 25 patients with nonmalignant diseases had increased FDC uptake. Sixteen lung cancer patients with mediastinal metastases had increased FDC uptake in the mediastinum, of whom three had no lymphadenopathy on computed tomography of the chest. Sixteen lung cancer patients without mediastinal nodal involvement had no FDG uptake in the mediastinum. Seven of these patients had lymphadenopathy on computed tomography. FDG-PET imaging is 100% accurate in predicting mediastinal involvement in patients with lung cancer. It is 100% sensitive and 52% specific in predicting the malignant nature of a chest radiographic abnormality. C1 W LOS ANGELES VET AFFAIRS MED CTR,PULM & CRIT CARE SECT,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,POSITRON EMMISS TOMOG SERV,LOS ANGELES,CA 90073. NR 19 TC 148 Z9 152 U1 0 U2 0 PU AMER LUNG ASSOC PI NEW YORK PA 1740 BROADWAY, NEW YORK, NY 10019 SN 1073-449X J9 AM J RESP CRIT CARE JI Am. J. Respir. Crit. Care Med. PD JAN PY 1996 VL 153 IS 1 BP 417 EP 421 PG 5 WC Critical Care Medicine; Respiratory System SC General & Internal Medicine; Respiratory System GA TP461 UT WOS:A1996TP46100061 PM 8542152 ER PT J AU Bradley, FM Hoover, HC Hulka, CA Whitman, GJ McCarthy, KA Hall, DA Moore, R Kopans, DB AF Bradley, FM Hoover, HC Hulka, CA Whitman, GJ McCarthy, KA Hall, DA Moore, R Kopans, DB TI The sternalis muscle: An unusual normal finding seen on mammography SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID BREAST-CANCER; CARCINOMA AB OBJECTIVE, We studied the appearance of the sternalis muscle on mammography and on CT and MR imaging,To our knowledge, this is the first description of this normal anatomic variant, An approach is provided that permits differentiation of the sternalis from significant pathology. SUBJECTS AND METHODS, Between January 1992 and December 1994, four women of an estimated 32,000 who had mammograms at the Massachusetts General Hospital had an unusual, irregular structure visible medially on the craniocaudal projection that posed a diagnostic dilemma. The records and imaging studies of these women and two others from the Deaconess Hospital breast imaging program were reviewed to determine the etiology of the findings seen by mammography and to establish a diagnostic approach, RESULTS, Surgery in one patient and cross-sectional imaging in the other five established that the structure was the sternalis muscle, Although it may be bilateral, the sternalis muscle was visible only unilaterally on the mammograms of these six women,The appearance of the muscle ranged from an irregularly rounded density at the sternal edge of the film to flame-shaped and almost completely surrounded by fat. CT and MR imaging are diagnostic when they show the longitudinal extent of the muscle, which lies anterior to the medial margin of the pectoralis major muscle. CONCLUSION. The sternalis muscle is an unusual variant of the chest wall musculature, It may be Visible as a rounded or irregular density on the craniocaudal mammogram along the sternal edge of the film. With improved mammographic positioning it will be seen more frequently, The muscle has a variety of appearances that should be familiar to the radiologist to avoid confusion with a malignant lesion, The etiology can be confirmed and cancer excluded by CT or MR imaging. C1 MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. NEW ENGLAND DEACONESS HOSP,DEPT RADIOL,BOSTON,MA 02215. NR 9 TC 38 Z9 42 U1 0 U2 1 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JAN PY 1996 VL 166 IS 1 BP 33 EP 36 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TL367 UT WOS:A1996TL36700009 PM 8571900 ER PT J AU McFarland, EG Kaufman, JA Saini, S Halpern, EF Lu, DSK Waltman, AC Warshaw, AL AF McFarland, EG Kaufman, JA Saini, S Halpern, EF Lu, DSK Waltman, AC Warshaw, AL TI Preoperative staging of cancer of the pancreas: Value of MB angiography versus conventional angiography in defecting portal venous invasion SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID MR-ANGIOGRAPHY; CARCINOMA; CT; LIVER; VEIN; RESECTABILITY; DIAGNOSIS AB OBJECTIVE, The purpose of this study was to compare contrast-enhanced MR angiography with conventional catheter angiography for detecting portal venous invasion in the preoperative staging of pancreatic cancer, using the surgical confirmation of vascular involvement as the standard of truth, SUBJECTS AND METHODS, MR and conventional angiography were performed in 20 patients with pancreatic carcinoma, with surgical confirmation in all cases, MR angiography was performed at 1.5 T, with coronal (2.9 mm) and axial (6.0 mm) contrast-enhanced breath-hold two-dimensional time-of-flight imaging, Data from each imaging technique were collected prospectively and analyzed in a blinded fashion by expert vascular radiologists, Vascular involvement in each patient and in each vessel (main portal vein, confluence, splenic vein, and superior mesenteric vein) determined whether the tumor was resectable (normal, abutment) or nonresectable (encased, occluded), Surgical confirmation of the vascular involvement of the portal venous structures was used as the standard of truth in all patients, RESULTS, Among the 20 patients, 11 tumors were surgically resectable and seven were nonresectable with performance of a palliative bypass, MR angiography and conventional angiography had an overall concordance in 65% of patients (13/20; seven resectable, four nonresectable, two false-negatives) on the basis of the vascular status in each patient of the portal venous structures and in 84% (47/56) of the individual vessels surgically confirmed. MR angiography correctly identified 11 of 11 resectable patients and five of nine nonresectable patients, with four false-negative cases, Conventional angiography correctly identified seven of 11 resectable patients and six of nine nonresectable patients, with three false-negative cases and four false-positive cases, CONCLUSION, The lack of false-positives by MR angiography suggests that MR imaging may provide a noninvasive screen for nonresectability on the basis of vascular involvement, with no patients with potentially resectable tumors being denied surgery by MR angiography in this cohort, However, the presence of false-negatives using MR angiography indicates the procedure would still not fully eliminate unnecessary laparotomies. C1 MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. NR 31 TC 51 Z9 53 U1 0 U2 2 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JAN PY 1996 VL 166 IS 1 BP 37 EP 43 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TL367 UT WOS:A1996TL36700010 PM 8571901 ER PT J AU Zerbey, AL Lee, MJ Brugge, WR Mueller, PR AF Zerbey, AL Lee, MJ Brugge, WR Mueller, PR TI Endoscopic sonography of the upper gastrointestinal tract and pancreas SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article AB Endoscopic sonography is a new approach to visualizing upper gastrointestinal lesions, The sonographic device, which incorporates a sonographic transducer mounted on a standard fiberoptic endoscope, makes it possible to obtain high-resolution images of mucosal and mural lesions of the gastrointestinal tract, as well as of adjacent masses. This technique has substantially improved preoperative staging of esophageal and gastric cancers. In addition, high-resolution sonographs of the pancreas can be obtained without degradation by overlying bowel gas, making identification of small, previously occult lesions of the pancreatic head possible,We have used endoscopic sonography in staging esophageal, gastric, and pancreatic cancers in more than 200 patients, This essay illustrates our experience with this new technique. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT RADIOL,BOSTON,MA 02114. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT MED,BOSTON,MA 02114. NR 6 TC 6 Z9 6 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JAN PY 1996 VL 166 IS 1 BP 45 EP 50 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TL367 UT WOS:A1996TL36700011 PM 8571902 ER PT J AU Lazar, EB Whitman, GJ Chew, FS AF Lazar, EB Whitman, GJ Chew, FS TI Embryonal rhabdomyosarcoma of the prostate SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Note C1 MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. NR 4 TC 5 Z9 5 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JAN PY 1996 VL 166 IS 1 BP 72 EP 72 PG 1 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TL367 UT WOS:A1996TL36700017 PM 8571909 ER PT J AU MayoSmith, WW Saini, S Slater, G Kaufman, JA Sharma, P Hahn, PF AF MayoSmith, WW Saini, S Slater, G Kaufman, JA Sharma, P Hahn, PF TI NIR contrast material for vascular enhancement: Value of superparamagnetic iron oxide SO AMERICAN JOURNAL OF ROENTGENOLOGY LA English DT Article ID MR ANGIOGRAPHY; AGENTS AB OBJECTIVE, The purposes of this study were to quantify abdominal vascular enhancement and to prove the feasibility of iron oxide-enhanced MR angiography in humans using three doses of superparamagnetic iron oxide agent AMI 227, SUBJECTS AND METHODS, Sixteen patients randomly received either 0.8, 1.1, or 1.7 mg Fe/kg of ultrasmall superparamagnetic iron oxide agent AMI 227. T1-weighted breath-hold gradient-echo images were obtained before and 45 min after IV administration of AMI 227, Signal intensity was measured in the aorta, the inferior vena cava, the portal vein, and muscle on unenhanced and contrast-enhanced images, Signal-to-noise ratios acid enhancement [(SNR after contrast-SNR before contrast) / SNR before contrast] were calculated, Vessels were visually graded before and after administration of AMI 227. RESULTS. All vessels showed statistically significant enhancement 45 min, after administration of AMI 227 by both qualitative and quantitative measures (p < .001). There was no significant increase in noise or signal intensity of muscle after contrast material was administered. The amount of enhancement was not statistically significantly different among the three doses, CONCLUSION, AMI 227, which is currently in phase III clinical trials, demonstrates significant vascular enhancement and may prove useful as an MR angiographic contrast agent. C1 MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. NR 18 TC 79 Z9 81 U1 0 U2 0 PU AMER ROENTGEN RAY SOC PI RESTON PA 1891 PRESTON WHITE DR SUBSCRIPTION FULFILLMENT, RESTON, VA 22091 SN 0361-803X J9 AM J ROENTGENOL JI Am. J. Roentgenol. PD JAN PY 1996 VL 166 IS 1 BP 73 EP 77 PG 5 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TL367 UT WOS:A1996TL36700018 PM 8571910 ER PT J AU Moldovan, S Livingston, E Zhang, RS Kleinman, R Guth, P Brunicardi, FC AF Moldovan, S Livingston, E Zhang, RS Kleinman, R Guth, P Brunicardi, FC TI Glucose-induced islet hyperemia is mediated by nitric oxide SO AMERICAN JOURNAL OF SURGERY LA English DT Article; Proceedings Paper CT 36th Annual Meeting of the Society-for-Surgery-of-the-Alimentary-Track CY MAY 14-17, 1995 CL SAN DIEGO, CA SP Soc Surg Alimentary Tract ID BLOOD-FLOW; SECRETION; RAT; PANCREAS; CELLS AB PURPOSE: TO determine whether hyperglycemia affects pancreatic islet microcirculation in vivo and whether nitric oxide is a mediator. METHODS: Islet blood flow was measured before and after infusion of glucose during in vivo microscopy of mouse pancreatic islet. The pancreas of male BALB/c mice was exteriorized and viewed under the microscope utilizing monochromatic transmitted light. The carotid artery and tail vein were cannulated and systemic blood pressure was monitored continuously. Under fluorescent light, a 0.02 mt bolus of 2% fluorescein isothyocyanate (FITC-albumin) was injected intra-arterially and the first pulse of FITC-albumin through an islet capillary was videorecorded. Following equilibration, either glucose or normal saline 300 mg/g of body weight was given intravenously. Five minutes later, a second bolus was given and the second pulse was videorecorded. The study was repeated in the presence of N omega-nitro-L-arginine methyl ester (L-NAME). The FITC-albumin bolus mean transit time (TT) and observed cross time (OCT) through the islet were calculated using slow-motion video analysis of the recorded images. RESULTS: Infusion of glucose resulted in a significant increase in islet blood flow with no change in systemic blood pressure: baseline TT was 20 +/- 1.3 pixel/0.03 sec and baseline OCT was 0.6 +/- 0.04 seconds; during hyperglycemia, TT was 16.1 +/- 1 pixel/0.03 sec, and OCT was 0.48 +/- 0.03 seconds (n = 11, P <0.05 versus basal via paired t-test). Continuous infusion of L-NAME negated the effect of hyperglycemia on islet blood flow: baseline TT was 20 +/- 1.8 pixel/0.03 sec and OCT was and 0.6 +/- 0.05 seconds; during hyperglycemia, TT was 20 +/- 1.1 pixel/0.03 sec and OCT was 0.6 +/- 0.33 seconds (n = 10; P <0.05 versus glucose via unpaired t-test). CONCLUSIONS: These data suggest that hyperglycemia results in increased islet capillary flow and nitric oxide is a regulator of islet blood flow during in vivo microscopy of the mouse islet. C1 UNIV CALIF LOS ANGELES,MED CTR,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,DEPT SURG,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,LOS ANGELES,CA 90073. FU NIDDK NIH HHS [1R29DK 46441-01] NR 21 TC 45 Z9 45 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD JAN PY 1996 VL 171 IS 1 BP 16 EP 20 DI 10.1016/S0002-9610(99)80066-X PG 5 WC Surgery SC Surgery GA TM835 UT WOS:A1996TM83500003 PM 8554133 ER PT J AU Yang, JM Southern, JF Warshaw, AL Lewandrowski, KB AF Yang, JM Southern, JF Warshaw, AL Lewandrowski, KB TI Proliferation tissue polypeptide antigen distinguishes malignant mucinous cystadenocarcinomas from benign cystic tumors and pseudocysts SO AMERICAN JOURNAL OF SURGERY LA English DT Article; Proceedings Paper CT 36th Annual Meeting of the Society-for-Surgery-of-the-Alimentary-Track CY MAY 14-17, 1995 CL SAN DIEGO, CA SP Soc Surg Alimentary Tract ID CARCINOEMBRYONIC ANTIGEN; PANCREATIC-CANCER; DIAGNOSIS; FLUID; CYSTADENOMA; NEOPLASMS; TPA AB BACKGROUND: Cystic lesions of the pancreas include inflammatory pseudocysts, serous cystadenomas, and mucinous cystic tumors, some of which are malignant. Previous studies have shown that malignant mucinous tumors differ from benign pancreatic cysts in proliferative activity, secretion of tumor markers, and expression of growth factor receptors. Analysis of aspirated cyst fluid for tumor markers, viscosity, and cytologic examination has been proposed as an aid to preoperative differential diagnosis. Tissue polypeptide antigen (TPA) is a soluble proliferation antigen produced by rapidly dividing tissues, including conventional ductal pancreatic carcinoma. TPA levels in pancreatic cyst fluids have not been reported. METHODS: Tissue polypeptide antigen levels were determined in 46 pancreatic cyst fluids using a commercial immunoassay technique. RESULTS: Mucinous cystadenocarcinomas exhibited significantly higher levels of cyst fluid TPA than benign cystic lesions, including pseudocysts, serous cystadenomas, and benign mucinous cystadenomas (mean 910,672 U/mL, median 300,900 U/mL, range 16,600 to 4,210,000 U/mL for malignant mucinous cystadenocarcinomas; versus mean 16,082 U/mL, median 2,455 U/mL, range 0 to 155,000 U/mL for benign cystic lesions considered as a group; P<0.0002). In 75% of the malignant cysts, the TPA values were in excess of 100,000 U/mL. Pseudocysts exhibited the lowest TPA levels (mean 2,108 U/mL, median 604 U/mL, range 0 to 20,240 U/mL) and were significantly lower than the values observed in cystic tumors (P<0.0005). Serous and benign mucinous tumors had intermediate levels of TPA. CONCLUSIONS: Elevated levels of the proliferation antigen TPA in malignant pancreatic cysts correlate with earlier observations of increased proliferative activity and overexpression of growth factor receptors in these tumors. The TPA measurement may be a useful addition to previously reported cyst fluid markers to aid in preoperative differential diagnosis. Markedly elevated or very low values indicate a malignant or benign cyst, respectively. C1 MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. NR 20 TC 11 Z9 11 U1 1 U2 1 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD JAN PY 1996 VL 171 IS 1 BP 126 EP 129 DI 10.1016/S0002-9610(99)80086-5 PG 4 WC Surgery SC Surgery GA TM835 UT WOS:A1996TM83500043 PM 8554126 ER PT J AU Rattner, DW Castillo, CFD Warshaw, AL AF Rattner, DW Castillo, CFD Warshaw, AL TI Pitfalls of distal pancreatectomy for relief of pain in chronic pancreatitis SO AMERICAN JOURNAL OF SURGERY LA English DT Article; Proceedings Paper CT 36th Annual Meeting of the Society-for-Surgery-of-the-Alimentary-Track CY MAY 14-17, 1995 CL SAN DIEGO, CA SP Soc Surg Alimentary Tract ID TISSUE; PANCREATICOJEJUNOSTOMY; PRESSURE AB PURPOSE: To examine whether preoperative computed tomography (CT) scans and pancreatograms can: (1) identify patients with chronic pancreatitis localized to the tail of the pancreas; and (2) select those patients who can obtain pain relief from a distal pancreatectomy. PATIENTS AND METHODS: Twenty patients were identified on whom the authors had performed distal pancreatectomy for relief of pain between January 1, 1991 and August 1, 1994. The results of surgery were classified as good, fair, or poor based on return to work and need for narcotics or rehospitalization. RESULTS: Eleven patients had good, 3 fair, and 6 poor results. All 7 patients with pseudocysts of the tail of the pancreas had good results, while 9 of 13 patients without pseudocysts had poor results. No other finding on CT scan, pancreatography, or laparotomy predicted successful pain relief by distal pancreatectomy. Furthermore, 3 patients had unexpected carcinoma found at the time of surgery. CONCLUSIONS: Even when anatomic evidence suggests that chronic pancreatitis primarily involves the tail of the pancreas and there is a structure of the midpancreatic duct that is believed to cause the symptoms, distal pancreatectomy seldom provides sustained pain relief. Unsuspected carcinoma of the body and tail of the pancreas occurs frequently in this subset of patients with chronic pancreatitis. C1 HARVARD UNIV,SCH MED,BOSTON,MA. RP Rattner, DW (reprint author), MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114, USA. NR 20 TC 54 Z9 55 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD JAN PY 1996 VL 171 IS 1 BP 142 EP 145 DI 10.1016/S0002-9610(99)80089-0 PG 4 WC Surgery SC Surgery GA TM835 UT WOS:A1996TM83500049 PM 8554129 ER PT J AU Wasa, M Bode, BP Souba, WW AF Wasa, M Bode, BP Souba, WW TI Adaptive regulation of amino acid transport: In nutrient deprived human hepatomas SO AMERICAN JOURNAL OF SURGERY LA English DT Article; Proceedings Paper CT 36th Annual Meeting of the Society-for-Surgery-of-the-Alimentary-Track CY MAY 14-17, 1995 CL SAN DIEGO, CA SP Soc Surg Alimentary Tract ID SYSTEM-A; HEPATOCELLULAR-CARCINOMA; RAT HEPATOCYTES; CELL LINES; GLUTAMINE; DEREPRESSION AB BACKGROUND: Malignant cells require increased amounts of amino acids, in particular glutamine and leucine, to support DNA and protein biosynthesis. Although plasma concentrations in the center of solid tumors can be much lower than normal circulating levels, it is still unknown how tumor cells can survive despite low amino acid levels. We examined the effects of glutamine or leucine deprivation on cell growth and, amino acid transport activity in two human hepatoma cell lines, SK-Hep and HepG2. METHODS: We studied the transport of glutamine, leucine, alanine, and arginine. The carrier-mediated uptake of H-3-amino acids was determined in cells cultured in normal and amino acid-deprived media. RESULTS: The growth of both cell lines was dependent on the concentration of glutamine and leucine. In SK-Hep, there was a significant increase in initial rate glutamine transport activity in the glutamine-derived group, attributable to an increase in transporter affinity (Km; 0.6 mmol/L [control], 385+/-43 mu mol/L; 0.1 mmol/L, 221+/-11 mu mol/L; P<0.01). At low glutamine concentration, the saturable Na+-independent uptake of leucine and arginine,as well as the Na+-dependent uptake of alanine increased significantly in both SK-Hep and HepG2. Similarly, in leucine-deprived SK-Hep cells, leucine uptake increased twofold, but the change was attributable to an enhanced transporter capacity (Vmax; 0.2 mmol/L [control], 38,900+/-700; 0.0 mmol/L, 75,900+/-4,900 pmol/mg protein per minute; P<0.001). CONCLUSIONS: Adaptive increases in initial rate amino acid transport activities were elicited by glutamine and leucine deprivation in these two human hepatoma cell lines. Decreased extracellular amino acid levels encountered by tumors in vivo may elicit similar adaptive responses that contribute to the maintenance of cytoplasmic levels of amino acids essential for growth. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DIV SURG ONCOL,BOSTON,MA 02114. FU NCI NIH HHS [R01 CA 57690] NR 21 TC 15 Z9 15 U1 0 U2 0 PU CAHNERS PUBL CO PI NEW YORK PA 249 WEST 17 STREET, NEW YORK, NY 10011 SN 0002-9610 J9 AM J SURG JI Am. J. Surg. PD JAN PY 1996 VL 171 IS 1 BP 163 EP 169 DI 10.1016/S0002-9610(99)80093-2 PG 7 WC Surgery SC Surgery GA TM835 UT WOS:A1996TM83500057 PM 8554134 ER PT B AU Orwoll, ES AF Orwoll, ES BE Oddens, BJ Vermeulen, A TI Epidemiology and diagnosis of osteoporosis in men SO ANDROGENS AND THE AGING MALE LA English DT Proceedings Paper CT Workshop on Androgens and the Aging Male CY DEC, 1995 CL GENEVA, SWITZERLAND SP Int Hlth Fdn RP Orwoll, ES (reprint author), PORTLAND VA MED CTR,BONE & MINERAL RES UNIT,POB 1034,PORTLAND,OR 97207, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PARTHENON PUBLISHING GROUP LTD PI LANCASTER PA CASTERTON HALL, CARNFORTH, LANCASTER, ENGLAND LA6 2LA BN 1-85070-763-4 PY 1996 BP 15 EP 37 PG 3 WC Andrology; Endocrinology & Metabolism; Urology & Nephrology SC Endocrinology & Metabolism; Urology & Nephrology GA BG96V UT WOS:A1996BG96V00002 ER PT J AU Nathan, DM AF Nathan, DM TI The pathophysiology of diabetic complications: How much does the glucose hypothesis explain? SO ANNALS OF INTERNAL MEDICINE LA English DT Article; Proceedings Paper CT 5th Regenstrief Conference on Risks and Benefits of Intensive Management in Non-Insulin-dependent Diabetes Mellitus CY DEC 11-13, 1994 CL WASHINGTON, DC SP Regemstroef Omst, NIH, NIDDKD, Ctr Dis Control & Prevent, Div Diabetes Translat ID ALDOSE REDUCTASE INHIBITOR; SORBITOL PATHWAY; BLOOD-PRESSURE; RETINOPATHY; PATHOGENESIS; MELLITUS; NEUROPATHY; ASSOCIATION; PROGRESSION; NEPHROPATHY AB Objective: To examine the putative pathogenetic mechanisms of the long-term, specific complications of diabetes mellitus. Data Sources: Literature review relevant to long-term diabetic complications and their pathogenesis. Study Selection: Studies of animal models of diabetes, epidemiologic investigations of diabetes and its long-term complications, and interventional studies examining intensive treatment of diabetes and its effect on the development and progression of complications. Data Synthesis: Diabetic retinopathy, nephropathy, and neuropathy occur in all clinical forms of diabetes mellitus, regardless of the cause of the diabetes. Hyperglycemia appears to be the major variable shared among these different clinical forms; and epidemiologic data, studies in animal models of diabetes, and the results of recent interventional studies such as the Diabetes Control and Complications Trial, all support an important and perhaps dominant role of hyperglycemia in the pathogenesis of complications. However, the diverse complications may not share the same pathogenesis. Different pathogenetic mechanisms may operate in different types of diabetic complications or at different stages of specific complications, or both. Conclusions: The level of chronic glycemia is the best established concomitant factor associated with diabetic complications. The mechanism by which hyperglycemia might cause complications remains unknown, and evidence for a uniform pathogenetic mechanism is far from established. C1 HARVARD UNIV,SCH MED,BOSTON,MA. RP Nathan, DM (reprint author), MASSACHUSETTS GEN HOSP,DIABET UNIT,BOSTON,MA 02114, USA. FU NCRR NIH HHS [RR01066-18]; NIDDK NIH HHS [UO1 DK30643-13] NR 52 TC 69 Z9 71 U1 0 U2 2 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JAN 1 PY 1996 VL 124 IS 1 BP 86 EP 89 PN 2 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA TL944 UT WOS:A1996TL94400002 PM 8554219 ER PT J AU Quickel, KE AF Quickel, KE TI Diabetes in a managed care system SO ANNALS OF INTERNAL MEDICINE LA English DT Article; Proceedings Paper CT 5th Regenstrief Conference on Risks and Benefits of Intensive Management in Non-Insulin-dependent Diabetes Mellitus CY DEC 11-13, 1994 CL WASHINGTON, DC SP Regemstroef Omst, NIH, NIDDKD, Ctr Dis Control & Prevent, Div Diabetes Translat ID SERVICE; OUTCOMES; SETTINGS; PROGRAM; FEE AB Health care expenditures account for over 14% of the gross domestic product in the United States. Managed care has evolved to control these costs. Because diabetes accounts for nearly 15% of health care expenditures, the strategies used by managed care organizations are expected to have a particular effect on diabetes. Managed care organizations have two primary goals: to control costs and to provide care of sufficient quality to attract and satisfy enrollees. Managed care organizations have designed strategies to meet these goals. Four primary managed care strategies and their effects on diabetes care are discussed: 1) payment incentives rewarding desired provider practice patterns; 2) designation of providers who possess desirable practice behaviors; 3) coverage policies that control the services paid for; and 4) traditional insurance strategies that determine who is eligible for insurance and what premiums are to be paid. The few direct studies of the effects of each strategy on the care of diabetic persons are discussed. The conclusion is that although managed care organizations have the potential to provide excellent care for diabetic persons, little evidence exists that they have improved either the quality or the cost-effectiveness of diabetes care. Recommendations to guide the development of cost-effective care for diabetic persons are presented. RP Quickel, KE (reprint author), JOSLIN DIABET CTR,1 JOSLIN PL,BOSTON,MA 02215, USA. NR 34 TC 26 Z9 26 U1 0 U2 0 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD JAN 1 PY 1996 VL 124 IS 1 BP 160 EP 163 PN 2 PG 4 WC Medicine, General & Internal SC General & Internal Medicine GA TL944 UT WOS:A1996TL94400016 PM 8554211 ER PT J AU Javitt, JC Aiello, LP AF Javitt, JC Aiello, LP TI Cost-effectiveness of detecting and treating diabetic retinopathy SO ANNALS OF INTERNAL MEDICINE LA English DT Article; Proceedings Paper CT 5th Regenstrief Conference on Risks and Benefits of Intensive Management in Non-Insulin-dependent Diabetes Mellitus CY DEC 11-13, 1994 CL WASHINGTON, D.C. SP Regemstroef Omst, NIH, NIDDKD, Ctr Dis Control & Prevent, Div Diabetes Translat ID 4-YEAR INCIDENCE; MELLITUS; DIAGNOSIS; AGE; PHOTOCOAGULATION; PROGRESSION; GUIDELINES; PREVALENCE; MORTALITY; SEVERITY AB Objective: To determine, from the health insurer's perspective, the cost of preventing vision loss in patients with diabetes mellitus through ophthalmologic screening and treatment and to calculate the cost-effectiveness of these interventions as compared with that of other medical interventions. Design: Computer modeling, incorporating data from population-based epidemiologic studies and multicenter clinical trials. Monte Carlo simulation was used, combined with sensitivity analysis and present value analysis of cost savings. Results: Screening and treatment of eye disease in patients with diabetes mellitus costs $3190 per quality-adjusted life-year (QALY) saved. This average cost is a weighted average (based on prevalence of disease) of the cost-effectiveness of detecting and treating diabetic eye disease in those with insulin-dependent diabetes mellitus ($1996 per QALY), those with non-insulin-dependent diabetes mellitus (NIDDM) who use insulin for glycemic control ($2933 per QALY), and those with NIDDM who do not use insulin for glycemic control ($3530 per QALY). Conclusions: Our analysis indicates that prevention programs aimed at improving eye care for diabetic persons not only result in substantial federal budgetary savings but are highly cost-effective health investments for society. Ophthalmologic screening for diabetic persons is more cost-effective than many routinely provided health interventions. Because diabetic eye disease is the leading cause of new cases of blindness among working-age Americans, these results support the widespread use of screening and treatment for diabetic eye disease. C1 JOSLIN DIABET CTR, BEETHAM EYE INST, BOSTON, MA 02215 USA. RP Javitt, JC (reprint author), GEORGETOWN UNIV, MED CTR, CTR SIGHT, 3800 RESERVOIR RD NW, WASHINGTON, DC 20007 USA. FU NEI NIH HHS [R01-EYO8805, R21-EY07744] NR 54 TC 208 Z9 212 U1 0 U2 8 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 USA SN 0003-4819 EI 1539-3704 J9 ANN INTERN MED JI Ann. Intern. Med. PD JAN 1 PY 1996 VL 124 IS 1 BP 164 EP 169 PN 2 PG 6 WC Medicine, General & Internal SC General & Internal Medicine GA TL944 UT WOS:A1996TL94400017 PM 8554212 ER PT J AU Shoulson, I Fahn, S Oakes, D Lang, A Langston, JW LeWitt, P Olanow, CW Penney, JB Tanner, C Kieburtz, K Koller, W Rodnitzky, R Fink, JS Growdon, JH Paulson, G Kurlan, R Friedman, JH Gancher, S Nutt, J Rajput, AH Bennett, JB Wooten, GF Goetz, C Shannon, K Suchowersky, O Brin, MF Bressman, SB Weiner, WJ SanchezRamos, J Jankovic, J Hoehn, M Tetrud, J Grimes, JD Pfeiffer, R Shults, C Thal, L Gauthier, S Golbe, LI Perlmutter, JS Moses, H Reich, SG Hurtig, HI Stern, M Barter, R VetereOverfield, B Gauger, L Malapira, T Dobson, J Atamian, S Tennis, M Cohen, JB Desclos, G Hoffman, E Denio, L Huber, S Woike, T Zoog, K Mendell, R Dudte, K Behr, J Gardiner, IF Lannon, M Carter, J Northrup, S Kanigan, B Turk, M Landow, E Schlick, P Mistura, K Carroll, VS Thelen, JA Demong, C Winfield, L Moskowitz, C Ingenito, A Sheldon, C Cornelius, L Heiberg, D Dunne, C Brady, J Kierans, C BelleScantlebury, L Duff, J Weber, H Savoini, D Lewis, P Kutner, SJ Gray, P Glaeske, C Hofman, R Pay, MM Salmon, D McFaul, F Amyot, D Bergen, M McGeeMinnich, L ODonnell, P Ferrise, S Shallow, K Baker, D Casaceli, C Eberly, S McDermott, M Nobel, R Orme, C Pelusio, RM Plumb, S Rudolph, A Randolph, H Sotack, J Watts, A AF Shoulson, I Fahn, S Oakes, D Lang, A Langston, JW LeWitt, P Olanow, CW Penney, JB Tanner, C Kieburtz, K Koller, W Rodnitzky, R Fink, JS Growdon, JH Paulson, G Kurlan, R Friedman, JH Gancher, S Nutt, J Rajput, AH Bennett, JB Wooten, GF Goetz, C Shannon, K Suchowersky, O Brin, MF Bressman, SB Weiner, WJ SanchezRamos, J Jankovic, J Hoehn, M Tetrud, J Grimes, JD Pfeiffer, R Shults, C Thal, L Gauthier, S Golbe, LI Perlmutter, JS Moses, H Reich, SG Hurtig, HI Stern, M Barter, R VetereOverfield, B Gauger, L Malapira, T Dobson, J Atamian, S Tennis, M Cohen, JB Desclos, G Hoffman, E Denio, L Huber, S Woike, T Zoog, K Mendell, R Dudte, K Behr, J Gardiner, IF Lannon, M Carter, J Northrup, S Kanigan, B Turk, M Landow, E Schlick, P Mistura, K Carroll, VS Thelen, JA Demong, C Winfield, L Moskowitz, C Ingenito, A Sheldon, C Cornelius, L Heiberg, D Dunne, C Brady, J Kierans, C BelleScantlebury, L Duff, J Weber, H Savoini, D Lewis, P Kutner, SJ Gray, P Glaeske, C Hofman, R Pay, MM Salmon, D McFaul, F Amyot, D Bergen, M McGeeMinnich, L ODonnell, P Ferrise, S Shallow, K Baker, D Casaceli, C Eberly, S McDermott, M Nobel, R Orme, C Pelusio, RM Plumb, S Rudolph, A Randolph, H Sotack, J Watts, A TI Impact of deprenyl and tocopherol treatment on Parkinson's disease in DATATOP subjects not requiring levodopa SO ANNALS OF NEUROLOGY LA English DT Article ID ANTIPARKINSON EFFICACY; SELEGILINE AB In the controlled trial Deprenyl and Tocopherol Antioxidative Therapy of Parkinsonism (DATATOP), 310 of the 800 enrolled subjects did not reach the primary end point of disability requiring levodopa therapy during 21 +/- 4 (mean +/- SD) months of observation or need early initiation of deprenyl (selegiline) during a 2-month withdrawal of experimental treatments. While maintaining the blindness of their original deprenyl and tocopherol treatment assignments, these 310 subjects were administered deprenyl 10 mg/day (open label) and were monitored systematically at 1- to 3-month intervals for up to 18 months (12 +/- 5 mo). During this extended trial, the 189 subjects who had been assigned originally to active deprenyl tended to reach the end point of disability faster than the 121 subjects who had not been assigned originally to deprenyl (hazard ratio, 1.43; 95% CI, 0.98, 2.09; p = 0.065). However, the differential rates of reaching the end point may have been due in part to the more severe baseline impairment of deprenyl-assigned subjects, who benefited originally from deprenyl but who were more likely to require levodopa during this extended period of observation. Prior treatment with deprenyl did not lead to superior survival with respect to the end point of disability requiring levodopa, suggesting that the initial advantages of deprenyl were not sustained. C1 COLUMBIA PRESBYTERIAN MED CTR,NEW YORK,NY 10032. TORONTO HOSP,TORONTO,ON M5T 2S8,CANADA. CALIF PARKINSONS INST,SUNNYVALE,CA. SINAI HOSP,DETROIT,MI 48235. UNIV S FLORIDA,TAMPA,FL. UNIV MICHIGAN,ANN ARBOR,MI 48109. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. RUSH PRESBYTERIAN ST LUKES MED CTR,CHICAGO,IL 60612. RP Shoulson, I (reprint author), UNIV ROCHESTER,MED CTR,DEPT NEUROL,BOX 673,601 ELMWOOD AVE,ROCHESTER,NY 14642, USA. RI Sanchez-Ramos, Juan/A-1188-2009 OI Sanchez-Ramos, Juan/0000-0002-3391-7857 NR 26 TC 121 Z9 123 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0364-5134 J9 ANN NEUROL JI Ann. Neurol. PD JAN PY 1996 VL 39 IS 1 BP 29 EP 36 PG 8 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA TT880 UT WOS:A1996TT88000005 ER PT J AU GomezIsla, T West, HL Rebeck, GW Harr, SD Growdon, JH Locascio, JJ Perls, TT Lipsitz, LA Hyman, BT AF GomezIsla, T West, HL Rebeck, GW Harr, SD Growdon, JH Locascio, JJ Perls, TT Lipsitz, LA Hyman, BT TI Clinical and pathological correlates of apolipoprotein E epsilon 4 in Alzheimer's disease SO ANNALS OF NEUROLOGY LA English DT Article ID AMYLOID PRECURSOR PROTEIN; BETA-PROTEIN; SENILE PLAQUES; TYPE-4 ALLELE; BINDING; DEPOSITION; FREQUENCY; DIAGNOSIS; SEVERITY; MUTATION AB Inheritance of the apolipoprotein E (apoE) epsilon 4 allele is associated with a high likelihood of developing Alzheimer's disease (AD). The pathophysiologic basis of this genetic influence is unknown. We reasoned that understanding the influence of apoE epsilon 4 on the clinical course and neuropathological features of AD may provide tests of potential mechanisms. We carried out a prospective longitudinal study to compare the age of onset, duration, and rate of progression of 359 AD patients to apoE genotype. Thirty-one of the individuals who died during the study were available for quantitative neuropathological evaluation. Statistically unbiased stereological counts of neurofibrillary tangles (NFTs) and A beta deposits were assessed in a high-order association cortex, the superior temporal sulcus. Analysis of clinical parameters compared with apoE genotype showed that the epsilon 4 allele is associated with an earlier age of onset but no change in rate of progression of dementia. Quantitative neuropathological assessment revealed that NFTs were strongly associated with clinical measures of dementia duration and severity but not with apoE genotype. A beta deposition, by contrast, was not related to clinical features but was elevated in association with apoE epsilon 4. These results indicate that apoE epsilon 4 is associated with selective clinical and neuropathological features of AD and support hypotheses that focus on an influence of apoE epsilon 4 on amyloid deposition. C1 MASSACHUSETTS GEN HOSP,NEUROL SERV,BOSTON,MA 02114. HEBREW REHABIL CTR AGED,BOSTON,MA 02131. DEACONESS HOSP,DEACONESS ELDERCARE,BOSTON,MA. FU NIA NIH HHS [AG08487, AG12406, P50 AG05134] NR 60 TC 287 Z9 293 U1 1 U2 4 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0364-5134 J9 ANN NEUROL JI Ann. Neurol. PD JAN PY 1996 VL 39 IS 1 BP 62 EP 70 DI 10.1002/ana.410390110 PG 9 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA TT880 UT WOS:A1996TT88000009 PM 8572669 ER PT J AU Canellos, GP AF Canellos, GP TI Current strategies for early Hodgkin's disease SO ANNALS OF ONCOLOGY LA English DT Article; Proceedings Paper CT 3rd International Symposium on Hodgkins Lymphoma CY SEP 18-23, 1995 CL COLOGNE, GERMANY SP Deut Krebshilfe, German Minist Res & Technol, Deut Forschungsgemeinsch, City Cologne, Nordrhein Westfalen, European Community DE early stages; Hodgkin's disease; prognostic factors; therapy; treatment options ID CLINICAL STAGE-I; PROGNOSTIC FACTORS; PLUS RADIOTHERAPY; CHEMOTHERAPY; VINBLASTINE; THERAPY; TRIAL; MOPP; ABVD; RADIATION AB A small percentage (similar to<10%) of patients present with minimal disease that is treatable with only mantle radiotherapy. The vast majority will present with apparent local disease but will have one or another poor prognostic features necessitating staging laparotomy if only mantle/para-aortic radiation therapy is the desired approach. Otherwise, more extensive radiation including splenic fields would be required or combined modality. The unanswered questions include: (1) definition of patients who require no adjunctive radiation therapy; (2) an assessment of the actual quantity of chemotherapy needed if complementary radiation therapy is to be added; and (3) determination of whether (67)gallium/SPECT scan can provide a measure of the efficacy of chemotherapy. Finally, can a less toxic regimen be substituted for ABVD in the treatment of early stage disease? Many of these questions are currently under investigation. RP Canellos, GP (reprint author), DANA FARBER CANC INST,DIV MED ONCOL,44 BINNEY ST,BOSTON,MA 02115, USA. NR 23 TC 3 Z9 3 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0923-7534 J9 ANN ONCOL JI Ann. Oncol. PY 1996 VL 7 SU 4 BP 91 EP 93 PG 3 WC Oncology SC Oncology GA UZ462 UT WOS:A1996UZ46200017 PM 8836417 ER PT J AU Netscher, DT Carlyle, T Thornby, J Bowen, D Harris, S Clamon, J AF Netscher, DT Carlyle, T Thornby, J Bowen, D Harris, S Clamon, J TI Hemostasis at skin graft donor sites: Evaluation of topical agents SO ANNALS OF PLASTIC SURGERY LA English DT Article AB Blood loss from split-thickness skin graft donor sites may be significant, various topical agents have been used to decrease this blood loss, including thrombin and epinephrine solutions of varying concentrations, We describe a K-Y jelly/epinephrine mixture that serves both as a lubricant for the dermatome and as a hemostatic agent, This mixture, in comparison with other topical agents, produces rapid hemostasis and offers the advantages of easy use, ready availability, and low cost, The blood loss savings based on this hemostatic technique is quantifiable and significant. C1 BAYLOR COLL MED,DIV PLAST SURG,HOUSTON,TX 77030. DEPT VET AFFAIRS MED CTR,PLAST SURG SECT,HOUSTON,TX. NR 6 TC 7 Z9 7 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0148-7043 J9 ANN PLAS SURG JI Ann. Plast. Surg. PD JAN PY 1996 VL 36 IS 1 BP 7 EP 10 DI 10.1097/00000637-199601000-00002 PG 4 WC Surgery SC Surgery GA TQ993 UT WOS:A1996TQ99300002 PM 8722976 ER PT J AU Concannon, MJ Duffy, FJ Palmer, WE May, JW AF Concannon, MJ Duffy, FJ Palmer, WE May, JW TI Late rupture of a flexor tendon after electrical injury: Tendon localization using magnetic resonance imaging. A case report SO ANNALS OF PLASTIC SURGERY LA English DT Article ID POSTERIOR TIBIAL TENDON; MRI DIAGNOSIS; ANKLE TENDONS; ANATOMY AB Rupture of a flexor tendon in the nonrheumatoid population is rare, Localizing the level of the tendon rupture can be a difficult task. We describe an individual who presented with the sudden inability to flex the index finger of the hand, which had been subjected to a severe electrical injury, Physical exam demonstrated lack of index finger flexion at the distal joint, The patient denied any pain or history of recent trauma. Preoperative magnetic resonance imaging (MRI) successfully located the proximal and distal ends of the ruptured flexor digitorum profundus tendon in the forearm, MRI allows for accurate preoperative assessment of tendon position and degree of retraction, thereby facilitating surgical planning and approach. Accurate localization of tendon pathology preoperatively minimizes unnecessary dissection, shortens the operative procedure, and clarifies operative planning. C1 HARVARD UNIV,SCH MED,BOSTON,MA. MASSACHUSETTS GEN HOSP,DEPT RADIOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DIV PLAST SURG,BOSTON,MA 02114. RP Concannon, MJ (reprint author), UNIV LOUISVILLE,DEPT SURG,DIV PLAST & RECONSTRUCT SURG,LOUISVILLE,KY 40292, USA. NR 20 TC 5 Z9 5 U1 0 U2 0 PU LITTLE BROWN CO PI BOSTON PA 34 BEACON STREET, BOSTON, MA 02108-1493 SN 0148-7043 J9 ANN PLAS SURG JI Ann. Plast. Surg. PD JAN PY 1996 VL 36 IS 1 BP 84 EP 87 DI 10.1097/00000637-199601000-00017 PG 4 WC Surgery SC Surgery GA TQ993 UT WOS:A1996TQ99300018 PM 8722991 ER PT J AU York, IA Rock, KL AF York, IA Rock, KL TI Antigen processing and presentation by the class I major histocompatibility complex SO ANNUAL REVIEW OF IMMUNOLOGY LA English DT Review DE class I major histocompatibility complex; antigen processing; antigen presentation; cytotoxic T lymphocyte; antigenic peptide ID TOXIC LYMPHOCYTES-T; ENDOGENOUSLY SYNTHESIZED PEPTIDE; ENDOPLASMIC-RETICULUM; CELL-SURFACE; INFLUENZA NUCLEOPROTEIN; HEAVY-CHAIN; MICROSOMAL-MEMBRANES; PROTEIN-DEGRADATION; SIGNAL SEQUENCE; VIRAL PEPTIDES AB Major histocompatibility complex (MHC) class I molecules bind peptides derived from cellular proteins and display them for surveillance by the immune system. These peptide-binding molecules are composed of a heavy chain, containing an antigen-binding groove, which is tightly associated with a light chain (beta(2)-microglobulin). The majority of presented peptides are generated by degradation of proteins in the cytoplasm, in many cases by a large multicatalytic proteolytic particle, the proteasome. Two beta-subunits of the proteasome, LMP2 and LMP7, are inducible by interferon-gamma and alter the catalytic activities of this particle, enhancing the presentation of at least some antigens. After production of the peptide in the cytosol, it is transported across the endoplasmic reticulum (ER) membrane in an ATP-dependent manner by TAP (transporter associated with antigen presentation), a member of the ATP-binding cassette family of transport proteins. There are minor pathways for generating presented peptides directly in the ER, and some evidence suggests that peptides may be further trimmed in this location. The class I heavy chain and beta(2)-microglobulin are cotranslationally translocated into the endoplasmic reticulum where their assembly may be facilitated by the sequential association of the heavy chain with chaperone proteins BiP and calnexin. The class I molecule then associates with the lumenal face of TAP where it is retained, presumably awaiting a peptide. After the class I molecule binds a peptide, it is released for exocytosis to the cell surface where cytotoxic T lymphocytes examine it for peptides derived from foreign proteins. RP York, IA (reprint author), DANA FARBER CANC INST,DEPT LYMPHOCYTE BIOL,44 BINNEY ST,BOSTON,MA 02215, USA. OI York, Ian/0000-0002-3478-3344 NR 189 TC 456 Z9 462 U1 2 U2 24 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0732-0582 J9 ANNU REV IMMUNOL JI Annu. Rev. Immunol. PY 1996 VL 14 BP 369 EP 396 DI 10.1146/annurev.immunol.14.1.369 PG 28 WC Immunology SC Immunology GA UH429 UT WOS:A1996UH42900016 PM 8717519 ER PT J AU Sleckman, BP Gorman, JR Alt, FW AF Sleckman, BP Gorman, JR Alt, FW TI Accessibility control of antigen-receptor variable-region gene assembly: Role of cis-acting elements SO ANNUAL REVIEW OF IMMUNOLOGY LA English DT Review DE accessibility; V(D)J recombination; lymphocyte development; transcriptional control elements ID IMMUNOGLOBULIN-KAPPA-GENE; HEAVY-CHAIN LOCUS; CHROMOSOMAL LOOP ANCHORAGE; CELL-SPECIFIC ENHANCER; BETA-GLOBIN GENE; V(D)J RECOMBINATION; B-CELLS; TRANSCRIPTIONAL REGULATION; DEVELOPMENTAL REGULATION; THYMOCYTE DEVELOPMENT AB Antigen receptor variable region genes are assembled from germline variable (V), diversity (D), and joining (J) gene segments. This process requires expression of V(D)J recombinase activity, and ''accessibility'' of variable gene segments to this recombinase. The exact mechanism by which variable gene segments become accessible during development is not known. However, several studies have shown that cis-acting elements that regulate transcription may also function to regulate accessibility. Here we review the evidence that transcriptional promoters, enhancers, and silencers are involved in regulation of accessibility. The manner in which these elements may combine to regulate accessibility is addressed. In addition, current and potential strategies for identifying and analyzing cis-acting elements that mediate locus accessibility are discussed. C1 HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115. CTR BLOOD RES,BOSTON,MA 02115. RP Sleckman, BP (reprint author), CHILDRENS HOSP,HOWARD HUGHES MED INST,BOSTON,MA 02115, USA. FU NIAID NIH HHS [AI01297-01, AI-35714, AI-20047] NR 119 TC 247 Z9 250 U1 0 U2 4 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0732-0582 J9 ANNU REV IMMUNOL JI Annu. Rev. Immunol. PY 1996 VL 14 BP 459 EP 481 DI 10.1146/annurev.immunol.14.1.459 PG 23 WC Immunology SC Immunology GA UH429 UT WOS:A1996UH42900019 PM 8717521 ER PT J AU Taylor, CR Sober, AJ AF Taylor, CR Sober, AJ TI Sun exposure and skin disease SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE adverse cutaneous ultraviolet effects ID ULTRAVIOLET-RADIATION; 1ST; PROTECTION; SUNSCREENS; SUNLIGHT; CANCER AB Sunlight exposure produces a variety of adverse cutaneous effects. Erythema, photosensitivity, and immunologic alterations represent acute events, whereas photoaging and carcinogenesis are long-term consequences. These adverse cutaneous sequelae can be minimized by photoprotection in the form of sun avoidance, regular cover-up with clothing, and sunscreen application. This chapter reviews the diagnosis and treatment of sun-related skin disorders and recommendations for reducing photodamage. RP Taylor, CR (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,DEPT DERMATOL,BOSTON,MA 02114, USA. NR 40 TC 39 Z9 39 U1 0 U2 1 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1996 VL 47 BP 181 EP 191 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA UE597 UT WOS:A1996UE59700017 PM 8712772 ER PT J AU Gusella, JF MacDonald, ME AF Gusella, JF MacDonald, ME TI Trinucleotide instability: A repeating theme in human inherited disorders SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE fragile X syndrome; myotonic dystrophy; spinobulbar muscular atrophy; Huntington's disease; spinocerebellar ataxia AB In recent years, a completely new mechanism of mutation has emerged in a number of disorders that display perplexing and paradoxical features of genetic inheritance. This mechanism involves the expansion and intergenerational instability of stretches of consecutive identical nucleotide triplets that also exist as shorter stable segments on normal chromosomes. The unstable nature of the trinucleotide segments has solved many of the genealogic puzzles in these disorders and has provided a new tool for predictive testing. Treatments for the disorders await a better understanding of the different pathogenic processes that are triggered by various expanded repeats. The existence of numerous other disorders with peculiarities of genetic inheritance suggests that this mutational mechanism may be a major cause of human inherited disease. RP Gusella, JF (reprint author), MASSACHUSETTS GEN HOSP EAST,MOLEC NEUROGENET UNIT,BOSTON,MA 02129, USA. NR 18 TC 68 Z9 68 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1996 VL 47 BP 201 EP 209 PG 9 WC Medicine, General & Internal SC General & Internal Medicine GA UE597 UT WOS:A1996UE59700019 PM 8712774 ER PT J AU Niles, JL AF Niles, JL TI Antineutrophil cytoplasmic antibodies in the classification of vasculitis SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE Wegener's granulomatosis; polyarteritis; pauci-immune; myeloperoxidase; proteinase 3 ID RAPIDLY PROGRESSIVE GLOMERULONEPHRITIS; MYELOID LYSOSOMAL-ENZYMES; WEGENERS GRANULOMATOSIS; CRESCENTIC GLOMERULONEPHRITIS; NEUTROPHIL CYTOPLASM; SYSTEMIC VASCULITIS; AUTOANTIBODIES; MYELOPEROXIDASE; DIAGNOSIS; SPECIFICITY AB Two important types of antineutrophil cytoplasmic antibodies (ANCA) have been identified: anti-proteinase 3 and anti-myeloperoxidase antibodies. In the appropriate clinical setting, the presence of either is virtually diagnostic of the subset of vasculitis that includes Wegener's granulomatosis, microscopic polyangiitis (microscopic polyarteritis), the Churg-Strauss syndrome, idiopathic pauci-immune necrotizing and crescentic glomerulonephritis, and related and overlapping forms of these vasculitidies. The finding of ANCA throughout this group identifies these syndromes as belonging to a single category or spectrum of disease. C1 MASSACHUSETTS GEN HOSP,DEPT PATHOL,IMMUNOPATHOL UNIT,BOSTON,MA 02114. RP Niles, JL (reprint author), MASSACHUSETTS GEN HOSP,DEPT MED,RENAL UNIT,BOSTON,MA 02114, USA. NR 32 TC 41 Z9 42 U1 0 U2 0 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1996 VL 47 BP 303 EP 313 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA UE597 UT WOS:A1996UE59700027 PM 8712783 ER PT J AU LloydJones, DM Bloch, KD AF LloydJones, DM Bloch, KD TI The vascular biology of nitric oxide and its role in atherogenesis SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE endothelium; endothelium-derived relaxing factor; neointimal formation; restenosis; signal transduction ID L-ARGININE; SMOOTH-MUSCLE; HYPERCHOLESTEROLEMIC PATIENTS; MONOCYTE CHEMOTAXIS; ENDOTHELIAL-CELLS; CORONARY-ARTERIES; SEPTIC SHOCK; CYCLIC-GMP; ACETYLCHOLINE; MECHANISMS AB Nitric oxide (NO), the biologically active component of endothelium-derived relaxing factor, has critical roles in the maintenance of vascular homeostasis. Decreased endothelial NO production, as a result of endothelial dysfunction, occurs in the early phases of atherosclerosis. NO appears to inhibit atherogenesis by inhibiting leukocyte and platelet activation and by inhibiting smooth muscle cell proliferation. Endothelial denudation is a prominent feature of vascular injury associated with percutaneous angioplasty, and decreased NO production appears to contribute to the restenosis process. Manipulation of the NO/cGMP signal transduction system may provide novel therapeutic approaches for limiting atherogenesis and neointimal proliferation in the future. C1 HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, CARDIOVASC RES CTR, BOSTON, MA 02114 USA. RP LloydJones, DM (reprint author), HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, CARDIAC UNIT, BOSTON, MA 02114 USA. RI Lloyd-Jones, Donald/C-5899-2009 NR 52 TC 166 Z9 170 U1 0 U2 6 PU ANNUAL REVIEWS PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 USA SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1996 VL 47 BP 365 EP 375 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA UE597 UT WOS:A1996UE59700031 PM 8712788 ER PT J AU Kahn, CR Vicent, D Doria, A AF Kahn, CR Vicent, D Doria, A TI Genetics of non-insulin-dependent (type-II) diabetes mellitus SO ANNUAL REVIEW OF MEDICINE LA English DT Review DE insulin secretion; insulin resistance; beta-cell; insulin receptor; IRS-1 ID GLUCOSE TRANSPORTER GLUT-4; GLYCOGEN-SYNTHASE GENE; HUMAN GLUCOKINASE GENE; RECEPTOR GENE; LINKAGE ANALYSIS; PIMA-INDIANS; MITOCHONDRIAL-DNA; FAMILIAL NIDDM; MESSENGER-RNA; POLYMORPHIC MICROSATELLITE AB Both genetic and environmental factors contribute to the etiology of non-insulin-dependent diabetes. The genetic component is heterogeneous and in some patients is probably complex, involving multiple genes. Specific genetic defects have been identified for rare monogenic forms of NIDDM: maturity-onset diabetes of the young, or MODY (which is due to glucokinase mutations in about 40% of families), syndromes of extreme insulin resistance (which often involve the insulin receptor), and diabetes-deafness syndromes (with defects in mitochondrial genes). In contrast, the genes involved in common forms of NIDDM are still uncertain. Mutations have been extensively searched in genes regulating insulin signaling and secretion. Some evidence of involvement has been produced for insulin-receptor substrate-1, glycogen synthase, the glucagon receptor, a ras-related protein (Rad), histocompatibility antigens, PC-1, and fatty acid binding protein, but the contribution of these genes to NIDDM is probably small. Other candidate genes (e.g. insulin, insulin receptor, glucose transporters) have been excluded as major diabetogenes. New insights are expected in the near future from the systematic scanning of the genome for linkage with NIDDM. RP Kahn, CR (reprint author), HARVARD UNIV,SCH MED,DEPT MED,JOSLIN DIABET CTR,RES DIV,BOSTON,MA 02215, USA. RI Vicent, David/O-2255-2014 OI Vicent, David/0000-0002-6663-0350 NR 157 TC 166 Z9 174 U1 1 U2 6 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4219 J9 ANNU REV MED JI Annu. Rev. Med. PY 1996 VL 47 BP 509 EP 531 PG 23 WC Medicine, General & Internal SC General & Internal Medicine GA UE597 UT WOS:A1996UE59700042 PM 8712800 ER PT J AU Cannon, SC AF Cannon, SC TI Sodium channel defects in myotonia and periodic paralysis SO ANNUAL REVIEW OF NEUROSCIENCE LA English DT Review DE muscle; ion channel; hyperkalemia; paramyotonia ID ADYNAMIA EPISODICA HEREDITARIA; ANEMONE ANEMONIA-SULCATA; MUSCLE CHLORIDE CHANNEL; III-IV-LINKER; SKELETAL-MUSCLE; PARAMYOTONIA-CONGENITA; FUNCTIONAL EXPRESSION; MOLECULAR-BASIS; QUARTER HORSES; NA+ CHANNELS AB Myotonias and periodic paralyses constitute a diverse group of inherited disorders of muscle in which the primary defect is an alteration in the electrical excitability of the muscle fiber. The ion channel defects underlying these excitability derangements have recently been elucidated at the molecular and functional levels. This review focuses on sodium channel mutations that disrupt inactivation and thereby cause both the enhanced excitability of myotonia (muscle stiffness due to repetitive discharges) and the inexcitability resulting from depolarization during attacks of paralysis. C1 MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114. RP Cannon, SC (reprint author), HARVARD UNIV,SCH MED,DEPT NEUROBIOL,BOSTON,MA 02114, USA. FU NIAMS NIH HHS [AR42703] NR 78 TC 66 Z9 66 U1 0 U2 1 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0147-006X J9 ANNU REV NEUROSCI JI Annu. Rev. Neurosci. PY 1996 VL 19 BP 141 EP 164 DI 10.1146/annurev.ne.19.030196.001041 PG 24 WC Neurosciences SC Neurosciences & Neurology GA TY518 UT WOS:A1996TY51800006 PM 8833439 ER PT J AU Simonian, NA Coyle, JT AF Simonian, NA Coyle, JT TI Oxidative stress in neurodegenerative diseases SO ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY LA English DT Review DE free radicals; Alzheimer's disease; Parkinson's disease; amyotrophic lateral sclerosis ID CEREBELLAR GRANULE CELLS; AMYOTROPHIC-LATERAL-SCLEROSIS; MITOCHONDRIAL ENERGY-METABOLISM; SUPEROXIDE-DISMUTASE ACTIVITY; NO SYNTHASE INHIBITION; NITRIC-OXIDE; ARACHIDONIC-ACID; PARKINSONS-DISEASE; LIPID-PEROXIDATION; PRIMARY CULTURES AB Oxidative stress refers to the cytopathologic consequences of a mismatch between the production of free radicals and the ability of the cell to defend against them. Growing data from experimental models and human brain studies suggest oxidative stress may play an important role in neuronal degeneration in diseases such as Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis. Mitochondrial oxidative metabolism, nitric oxide, phospholipid metabolism, and proteolytic pathways are potential sources of intracellular free radicals. Alterations in free radical defense systems may also contribute to oxidative stress. A net increase in reactive oxygen species can produce damage to lipids, proteins, and DNA and induce necrosis or apoptosis. Elucidating the pathways important in the production of and defense from free radicals may be important in devising new pharmacologic strategies to slow or halt neuronal degeneration. C1 MASSACHUSETTS GEN HOSP, DEPT PSYCHIAT, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. RP MASSACHUSETTS GEN HOSP, DEPT NEUROL, BOSTON, MA 02114 USA. NR 177 TC 757 Z9 798 U1 6 U2 44 PU ANNUAL REVIEWS PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 USA SN 0362-1642 EI 1545-4304 J9 ANNU REV PHARMACOL JI Annu. Rev. Pharmacol. Toxicol. PY 1996 VL 36 BP 83 EP 106 DI 10.1146/annurev.pa.36.040196.000503 PG 24 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA UH635 UT WOS:A1996UH63500004 PM 8725383 ER PT J AU Myers, MG White, MF AF Myers, MG White, MF TI Insulin signal transduction and the IRS proteins SO ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY LA English DT Review DE cytokines; SH2 domains; diabetes; tyrosine phosphorylation; tyrosine kinases ID GROWTH-FACTOR RECEPTOR; TYROSINE KINASE-ACTIVITY; COOH-TERMINAL TRUNCATION; T-CELL ACTIVATION; PHOSPHATIDYLINOSITOL 3-KINASE; BETA-SUBUNIT; BINDING-SITE; SH2 DOMAIN; PHOSPHOTYROSINE PROTEIN; JUXTAMEMBRANE REGION AB Insulin controls organismal and cellular physiology by initiating numerous intracelluar signals. Insulin first binds the extracelluar domain of the insulin receptor, which activates the receptor's intracellular tyrosine kinase. Receptor-mediated phosphorylation of the IRS proteins is required for the propagation of signals for mitogenesis, glucose transport, and numerous other biological and biochemical events during insulin signaling. IRS proteins also mediate signaling by a subset of other growth factor and cytokine receptors; recognition and phosphorylation by specific receptors appears to be mediated by the PH and PTB domains of the IRS proteins. The best understood mechainsm of IRS-protein-mediated signaling is the binding of SH2 domain-containing signaling molecules (such as PI 3'-kinase) by tyrosine phosphorylation sites on IRS proteins. Other paradigms of IRS-protein signaling are beginning to emerge, however, and these exciting molecules promise to teach us much in the next few years. C1 HARVARD UNIV,SCH MED,PROGRAM BIOMED & BIOL SCI,BOSTON,MA 02215. HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02215. RP Myers, MG (reprint author), HARVARD UNIV,SCH MED,JOSLIN DIABET CTR,DIV RES,BOSTON,MA 02215, USA. FU NIDDK NIH HHS [DK 3871, DK 43808] NR 231 TC 260 Z9 264 U1 0 U2 4 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0362-1642 J9 ANNU REV PHARMACOL JI Annu. Rev. Pharmacol. Toxicol. PY 1996 VL 36 BP 615 EP 658 PG 44 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA UH635 UT WOS:A1996UH63500025 PM 8725404 ER PT J AU Sands, BE Podolsky, DK AF Sands, BE Podolsky, DK TI The trefoil peptide family SO ANNUAL REVIEW OF PHYSIOLOGY LA English DT Review DE ITF; pS2; spasmolytic polypeptide; mucin glycoprotein; wound healing ID HUMAN-BREAST-CANCER; PANCREATIC SPASMOLYTIC POLYPEPTIDE; EPIDERMAL GROWTH-FACTOR; CELL-LINE MCF-7; AMINO-ACID-SEQUENCE; XENOPUS-LAEVIS SKIN; RESPONSIVE GENE PS2; MESSENGER-RNA; P-DOMAIN; INTEGUMENTARY MUCIN AB The unique three-loop structure of the trefoil motif, formed by intrachain disulfide bonds in a 1-5, 2-4, 3-6 configuration between six conserved cysteine residues, is the defining feature of a recently recognized family of peptides. Expression of trefoil peptides is closely related to that of mucin glycoproteins in diverse biological sources. Three distinct members of the family (pS2, intestinal trefoil factor, and spasmolytic polypeptide) are produced in the mammalian gastrointestinal tract by mucus-secreting cells and targeted primarily for luminal secretion. The compact structure of the trefoil motif may be responsible for marked resistance of trefoil peptides to proteolytic digestion, enabling them to function in the harsh environment of the gastrointestinal lumen. Trefoil peptides are ectopically expressed adjacent to areas of inflammation within the gastrointestinal tract and may play an important role in both maintaining the barrier function of mucosal surfaces and facilitating healing after injury. C1 MASSACHUSETTS GEN HOSP,CTR STUDY INFLAMMATORY BOWEL DIS,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. RP Sands, BE (reprint author), MASSACHUSETTS GEN HOSP,GASTROINTESTINAL UNIT,BOSTON,MA 02114, USA. NR 87 TC 171 Z9 190 U1 1 U2 7 PU ANNUAL REVIEWS INC PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 SN 0066-4278 J9 ANNU REV PHYSIOL JI Annu. Rev. Physiol. PY 1996 VL 58 BP 253 EP 273 DI 10.1146/annurev.physiol.58.1.253 PG 21 WC Physiology SC Physiology GA UB984 UT WOS:A1996UB98400013 PM 8815795 ER PT J AU Cornish, JW OBrien, CP AF Cornish, JW OBrien, CP TI Crack cocaine abuse: An epidemic with many public health consequences SO ANNUAL REVIEW OF PUBLIC HEALTH LA English DT Article DE substance abuse/dependence; toxicity; epidemiology; HIV; pregnancy ID METHADONE-MAINTAINED PATIENTS; PREGNANCY; COCAETHYLENE; DESIPRAMINE; DEPENDENCE; PREVALENCE; ABSTINENCE; CONCURRENT; MECHANISMS; BEHAVIORS AB In the mid-1980s a new, smokable form of cocaine, called crack, was introduced in the United States. Soon thereafter, it became apparent that crack cocaine abuse was a serious and important public health concern. Over the past several years, crack cocaine use has increasingly been associated with a myriad of immediate and long-term adverse effects. During this same period, crack cocaine use has progressively moved away from experimentation and recreational use to chronic and compulsive drug use. C1 DEPT VET AFFAIRS MED CTR, PHILADELPHIA, PA 19104 USA. RP Cornish, JW (reprint author), UNIV PENN, DEPT PSYCHIAT, PHILADELPHIA, PA 19104 USA. NR 54 TC 58 Z9 60 U1 7 U2 13 PU ANNUAL REVIEWS PI PALO ALTO PA 4139 EL CAMINO WAY, PO BOX 10139, PALO ALTO, CA 94303-0139 USA SN 0163-7525 J9 ANNU REV PUBL HEALTH JI Annu. Rev. Public Health PY 1996 VL 17 BP 259 EP 273 PG 15 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA UK766 UT WOS:A1996UK76600016 PM 8724227 ER PT J AU Rusconi, S Moonis, M Merrill, DP Pallai, PV Neidhardt, EA Singh, SK Willis, KJ Osburne, MS Profy, AT Jenson, JC Hirsch, MS AF Rusconi, S Moonis, M Merrill, DP Pallai, PV Neidhardt, EA Singh, SK Willis, KJ Osburne, MS Profy, AT Jenson, JC Hirsch, MS TI Naphthalene sulfonate polymers with CD4-blocking and anti-human immunodeficiency virus type 1 activities SO ANTIMICROBIAL AGENTS AND CHEMOTHERAPY LA English DT Article ID MOLECULAR-WEIGHT; DEXTRAN SULFATE; HIV ACTIVITIES; HTLV-III; AIDS; INHIBITORS; RETROVIRUS; INFECTION; RECEPTOR; BINDING AB PIC 024-4 and PRO 2000 are naphthalene sulfonate polymers that bind to CD4 with nanomolar affinity and block binding of gp120. Both have activity against human immunodeficiency virus type 1 in 1-19 cells, peripheral blood mononuclear cells, and primary monocyte/macrophages, are synergistic with zidovudine, and do not inhibit tetanus toxoid-stimulated T-cell proliferation at anti-human immunodeficiency virus type 1 concentrations. C1 MASSACHUSETTS GEN HOSP,INFECT DIS UNIT,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. PROCEPT INC,CAMBRIDGE,MA 02139. RI Rusconi, Stefano/H-9263-2012 FU FIC NIH HHS [D42 TW00004] NR 21 TC 120 Z9 126 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0066-4804 J9 ANTIMICROB AGENTS CH JI Antimicrob. Agents Chemother. PD JAN PY 1996 VL 40 IS 1 BP 234 EP 236 PG 3 WC Microbiology; Pharmacology & Pharmacy SC Microbiology; Pharmacology & Pharmacy GA TN213 UT WOS:A1996TN21300046 PM 8787913 ER PT J AU deJong, MD Boucher, CAB Galasso, GJ Hirsch, MS Kern, ER Lange, JMA Richman, DD AF deJong, MD Boucher, CAB Galasso, GJ Hirsch, MS Kern, ER Lange, JMA Richman, DD TI Consensus symposium on combined antiviral therapy SO ANTIVIRAL RESEARCH LA English DT Article; Proceedings Paper CT Consensus Symposium on Combined Antiviral Therapy CY JUL 25-27, 1995 CL LISBON, PORTUGAL SP Macrae Grp, New York, Int Soc Antiviral Res, NIAID ID HUMAN-IMMUNODEFICIENCY-VIRUS; CHRONIC HEPATITIS-C; PLACEBO-CONTROLLED TRIAL; RECOMBINANT INTERFERON-ALPHA; POLYMERASE CHAIN-REACTION; HUMAN LIVER-MICROSOMES; REVERSE-TRANSCRIPTASE; CYTOMEGALO-VIRUS; COMBINATION THERAPY; REPLICATION INVITRO C1 UNIV UTRECHT,EIKMAN WINKLER INST,ANTIVIRAL THERAPY LAB,UTRECHT,NETHERLANDS. NIH,BETHESDA,MD 20892. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,INFECT DIS UNIT,BOSTON,MA. UNIV ALABAMA,DEPT PEDIAT,BIRMINGHAM,AL. UNIV CALIF SAN DIEGO,DEPT PATHOL,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,SAN DIEGO VET AFFAIRS MED CTR,LA JOLLA,CA 92093. RP deJong, MD (reprint author), UNIV AMSTERDAM,ACAD MED CTR,NATL AIDS THERAPY EVALUAT CTR,DEPT INFECT DIS TROP MED & AIDS,1105 AZ AMSTERDAM,NETHERLANDS. NR 126 TC 6 Z9 6 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-3542 J9 ANTIVIR RES JI Antiviral Res. PD JAN PY 1996 VL 29 IS 1 BP 5 EP 29 DI 10.1016/0166-3542(95)00910-8 PG 25 WC Pharmacology & Pharmacy; Virology SC Pharmacology & Pharmacy; Virology GA UD272 UT WOS:A1996UD27200002 PM 8721539 ER PT J AU Richman, H Frueh, BC AF Richman, H Frueh, BC TI Personality disorder symptomatology among Vietnam veterans with combat-related PTSD SO ANXIETY LA English DT Article DE PTSD; Vietnam veterans; personality; SCID-II self-report; anxiety disorders; major depression ID POSTTRAUMATIC-STRESS-DISORDER; AGORAPHOBIC OUTPATIENTS; ANXIETY DISORDER; QUESTIONNAIRE; DEPRESSION; SAMPLE; MCMI; PREVALENCE; VALIDITY; ILLNESS AB This research examined self-report personality profiles of 42 Vietnam veterans with combat-related posttraumatic stress disorder (PTSD) evaluated at an outpatient Veteran's Administration hospital PTSD clinic. Assessment was via the Structured Clinical Interview for the Diagnostic and Statistical Manual of Mental Disorders (3rd ed., rev; DSM-III-R) Personality Disorders-II (SCID-II) self-report. Self-reported personality disorder symptomatology of PTSD patients was contrasted with that of 51 outpatients with a primary diagnosis of an anxiety disorder other than PTSD and with 16 patients with a primary diagnosis of major depressive disorder (MDD). Symptomatology from each of the 11 DSM-III-R categories and from the three personality disorder ''clusters'' was calculated in terms of percentage of possible traits endorsed, thus creating personality ''profiles'' for the three groups. PTSD veterans endorsed more traits overall than did both the mixed anxiety and MDD groups, particularly on the Cluster A, avoidant, and borderline scales. Results suggest a PTSD-related personality profile characterized by emotional lability/poor anger control, paranoia/suspiciousness, identity disturbance/confusion, social withdrawal/avoidance, and feelings of emptiness and boredom. (C) 1996 Wiley-Liss, Inc. C1 MED UNIV S CAROLINA,DEPT PSYCHIAT & BEHAV SCI,CHARLESTON,SC 29425. MED UNIV S CAROLINA,RALPH H JOHNSON VET AFFAIRS MED CTR,DEPT PSYCHIAT & BEHAV SCI,CHARLESTON,SC 29425. NR 51 TC 11 Z9 11 U1 1 U2 5 PU JOHN WILEY & SONS INC PI NEW YORK PA 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1070-9797 J9 ANXIETY JI Anxiety PY 1996 VL 2 IS 6 BP 286 EP 295 PG 10 WC Psychiatry; Psychology SC Psychiatry; Psychology GA WA038 UT WOS:A1996WA03800005 PM 9160636 ER PT S AU Hyman, BT GomezIsla, T Rebeck, GW Briggs, M Chung, HY West, HL Greenberg, S Mui, S Nichols, S Wallace, R Growdon, JH AF Hyman, BT GomezIsla, T Rebeck, GW Briggs, M Chung, HY West, HL Greenberg, S Mui, S Nichols, S Wallace, R Growdon, JH BE Relkin, NR Khachaturian, Z Gandy, S TI Epidemiological, clinical, and neuropathological study of apolipoprotein E genotype in Alzheimer's disease SO APOLIPOPROTEIN E GENOTYPING IN ALZHEIMER'S DISEASE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Apolipoprotein E Genotyping in Alzheimers Disease CY OCT 21-22, 1995 CL CHICAGO, IL SP NIA, Alzheimers Assoc ID EPSILON-4 ALLELE; FREQUENCY C1 UNIV IOWA,COLL MED,DEPT PREVENTAT MED,IOWA CITY,IA 52242. RP Hyman, BT (reprint author), MASSACHUSETTS GEN HOSP,NEUROL SERV,WRN 408,BOSTON,MA 02114, USA. FU NIA NIH HHS [AG02106, AG12406, P50 AG05134] NR 14 TC 45 Z9 48 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-048-4 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 802 BP 1 EP 5 DI 10.1111/j.1749-6632.1996.tb32592.x PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA BH23P UT WOS:A1996BH23P00001 PM 8993478 ER PT S AU Haines, JL Pritchard, ML Saunders, AM Schildkraut, JM Growdon, JH Gaskell, PC Farrer, LA Auerbach, SA Gusella, JF Locke, PA Rosi, BL Yamaoka, L Small, GW Conneally, PM Roses, AD PericakVance, M AF Haines, JL Pritchard, ML Saunders, AM Schildkraut, JM Growdon, JH Gaskell, PC Farrer, LA Auerbach, SA Gusella, JF Locke, PA Rosi, BL Yamaoka, L Small, GW Conneally, PM Roses, AD PericakVance, M BE Relkin, NR Khachaturian, Z Gandy, S TI No association between alpha 1-antichymotrypsin and familial Alzheimer's disease SO APOLIPOPROTEIN E GENOTYPING IN ALZHEIMER'S DISEASE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Apolipoprotein E Genotyping in Alzheimers Disease CY OCT 21-22, 1995 CL CHICAGO, IL SP NIA, Alzheimers Assoc ID APOLIPOPROTEIN-E; AMYLOID DEPOSITS; TYPE-4 ALLELE; EARLY-ONSET; GENE; PROTEIN; LINKAGE; RISK C1 MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114. DUKE UNIV,MED CTR,DUKE COMPREHENS CANC CTR,DURHAM,NC 27710. BOSTON UNIV,MED CTR,DEPT NEUROL,BOSTON,MA 02118. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. INDIANA UNIV,MED CTR,DEPT MED & MOL GENET,INDIANAPOLIS,IN 46202. RP Haines, JL (reprint author), MASSACHUSETTS GEN HOSP,MOL NEUROGENET UNIT,CHARLESTOWN,MA 02129, USA. RI Haines, Jonathan/C-3374-2012; OI Farrer, Lindsay/0000-0001-5533-4225 FU NIA NIH HHS [AG05128, U24 AG021886]; NINDS NIH HHS [NS26630, NS31153] NR 21 TC 10 Z9 10 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-048-4 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 802 BP 35 EP 41 DI 10.1111/j.1749-6632.1996.tb32596.x PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA BH23P UT WOS:A1996BH23P00005 PM 8993482 ER PT S AU Small, GW Komo, S LaRue, A Saxena, S Phelps, ME Mazziotta, JC Saunders, AM Haines, JL PericakVance, MA Roses, AD AF Small, GW Komo, S LaRue, A Saxena, S Phelps, ME Mazziotta, JC Saunders, AM Haines, JL PericakVance, MA Roses, AD BE Relkin, NR Khachaturian, Z Gandy, S TI Early detection of Alzheimer's disease by combining apolipoprotein E and neuroimaging SO APOLIPOPROTEIN E GENOTYPING IN ALZHEIMER'S DISEASE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Apolipoprotein E Genotyping in Alzheimers Disease CY OCT 21-22, 1995 CL CHICAGO, IL SP NIA, Alzheimers Assoc ID POSITRON EMISSION TOMOGRAPHY; CEREBRAL GLUCOSE-METABOLISM; TEMPORAL-LOBE ATROPHY; E TYPE-4 ALLELE; SENILE DEMENTIA; GRAY-MATTER; GENE; DIAGNOSIS; RISK; PREVALENCE C1 UNIV CALIF LOS ANGELES,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT MOL & MED PHARMACOL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,DEPT RADIOL SCI,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,CTR AGING,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV NEW MEXICO,DEPT PSYCHIAT,ALBUQUERQUE,NM 87106. DUKE UNIV,MED CTR,DIV NEUROL,DURHAM,NC 27710. DUKE UNIV,MED CTR,DIV NEUROBIOL,DURHAM,NC 27710. DUKE UNIV,MED CTR,JOSEPH & KATHLEEN BRYAN ALZHEIMERS DIS RES CTR,DURHAM,NC 27710. MASSACHUSETTS GEN HOSP,MOL NEUROGENET UNIT,CHARLESTOWN,MA 02129. UNIV CALIF LOS ANGELES,ALZHEIMERS DIS CTR,LOS ANGELES,CA 90024. RI Haines, Jonathan/C-3374-2012 FU NIA NIH HHS [1R01 AG13308, AG10123]; NIMH NIH HHS [1R01 MH52453] NR 45 TC 53 Z9 56 U1 1 U2 3 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-048-4 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 802 BP 70 EP 78 DI 10.1111/j.1749-6632.1996.tb32600.x PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA BH23P UT WOS:A1996BH23P00009 PM 8993486 ER PT J AU Lipsitz, SR Fitzmaurice, GM Molenberghs, G AF Lipsitz, SR Fitzmaurice, GM Molenberghs, G TI Goodness-of-fit tests for ordinal response regression models SO APPLIED STATISTICS-JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES C LA English DT Article DE discrete response; Hosmer-Lemeshow statistic; residuals; score test AB In this paper, goodness-of-fit test statistics for ordinal regression models are proposed, which have approximate chi(2)-distributions when the model has been correctly specified. The statistics proposed can be viewed as extensions of the Hosmer-Lemeshow statistic to ordinal categorical data and can be easily calculated by using existing statistical software for analysing ordinal response data The methods are illustrated by using data from an arthritis clinical trial comparing the drug auranofin and placebo therapy for the treatment of rheumatoid arthritis, in which the response is a self-assessment of arthritis, classified as poor, fair and good. The covariates of interest are age, gender, treatment and base-line response. A proportional odds model is fitted to the data, and the proposed goodness-of-fit statistics are applied to the fitted model. Also, the small sample properties of the proposed goodness-of-fit statistics are compared in a simulation study. C1 HARVARD UNIV,SCH PUBL HLTH,BOSTON,MA 02115. LIMBURGS UNIV CENTRUM,B-3610 DIEPENBEEK,BELGIUM. RP Lipsitz, SR (reprint author), DANA FARBER CANC INST,DIV BIOSTAT & EPIDEMIOL,44 BINNEY ST,BOSTON,MA 02115, USA. NR 16 TC 16 Z9 16 U1 0 U2 3 PU BLACKWELL PUBL LTD PI OXFORD PA 108 COWLEY RD, OXFORD, OXON, ENGLAND OX4 1JF SN 0035-9254 J9 APPL STAT-J ROY ST C JI Appl. Stat.-J. R. Stat. Soc. PY 1996 VL 45 IS 2 BP 175 EP 190 DI 10.2307/2986153 PG 16 WC Statistics & Probability SC Mathematics GA TZ492 UT WOS:A1996TZ49200004 ER PT J AU Weng, SA Spiro, RG AF Weng, SA Spiro, RG TI Endoplasmic reticulum kifunensine-resistant alpha-mannosidase is enzymatically and immunologically related to the cytosolic alpha-mannosidase SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article DE glycoprotein processing; ER alpha-mannosidase; cytosolic alpha-mannosidase; N-linked oligosaccharides; kifunensine; mannosidase inhibitors; 1,4-dideoxy-1,4-imino-D-mannitol ID N-LINKED OLIGOSACCHARIDES; RAT-LIVER; GLYCOPROTEIN-BIOSYNTHESIS; TRIMMING MAN9-MANNOSIDASE; SACCHAROMYCES-CEREVISIAE; PROCESSING MANNOSIDASE; PROTEIN TRANSLOCATION; POLYACRYLAMIDE GELS; PURIFICATION; MEMBRANE AB Studies were undertaken to evaluate the relationship of the recently described (S. Weng and R. G. Spiro, 1993, J. Biol. Chem. 268, 25656-25663) rat liver kifunensine (KIF)-resistant mannosidase (ER mannosidase II) to the mannose-trimming enzyme of cytosol, We observed that the ER mannosidase II manifests a large number of catalytic and immunological properties similar to those of the cytosolic alpha-mannosidase, which contrast with the quite different characteristics of the KIF-sensitive enzyme (ER mannosidase I). In addition to a mutual resistance to KIF inhibition, the cytosolic enzyme and ER mannosidase II have comparable susceptibility to blocking by swainsonine and 1,4-dideoxy-1,4-imino-D-mannitol, and the latter agent was found to function effectively both in vitro and in vivo. The cytosolic and ER II mannosidases were alike in specifically excising the terminal mannose of the alpha 1,6-linked chain of Man(9)GlcNAc to yield Man(8)GlcNAc isomer C; in preferentially hydrolyzing polymannose-GlcNAc(1) over polymannose-GlcNAc(2) substrates; and in cleaving p-nitrophenyl alpha-D-mannoside. An immunological cross-reactivity between cytosolic mannosidase (M(r) 105 kDa) and ER mannosidase II (M(r) 82 kDa), neither of which is N-glycosylated, was established, suggesting that the latter is translocated posttranslationally into the lumen of the ER compartment in which we found it to be present as a soluble protein. Since antibodies directed against a sequence near the C-terminal end of the cytosolic enzyme reacted with ER mannosidase II while those against a sequence close to the N-terminus did not, it is likely that a proteolytic cleavage of the latter segment takes place during or after translocation. The absence in ER mannosidase II of the pronounced cobalt activation of the cytosolic enzyme suggests that the portion of the polypeptide chain removed during the 105- to 82-kDa conversion includes the binding domain for this ion. (C) 1996 Academic Press, Inc. C1 JOSLIN DIABET CTR,BOSTON,MA 02215. HARVARD UNIV,SCH MED,DEPT BIOL CHEM,BOSTON,MA 02215. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02215. FU NIDDK NIH HHS [DK 17477] NR 55 TC 66 Z9 68 U1 0 U2 2 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD JAN 1 PY 1996 VL 325 IS 1 BP 113 EP 123 DI 10.1006/abbi.1996.0014 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA TP810 UT WOS:A1996TP81000014 PM 8554335 ER PT J AU Baden, HP Kollias, N Anderson, RR Hopkins, T Raftery, L AF Baden, HP Kollias, N Anderson, RR Hopkins, T Raftery, L TI Drosophila melanogaster larvae detect low doses of UVC radiation as manifested by a writhing response SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE UVR; Drosophila melanogaster; larvae; UVC; behavioral response; catecholamines ID INSECT CUTICLE; HUMAN-SKIN; SCLEROTIZATION; MELANOGENESIS; CELLS; DOPA AB Exposure of insects and higher orders of animals to UVC radiation has been shown to result in toxicity with a delayed expression. We have observed an immediate writhing response in slowly wandering third instar larvae of Drosophila melanogaster exposed to UVC radiation at doses that were not lethal. UVB and UVA radiation had no effect. Mutants for the visual and sensory systems appeared to respond normally, but CO2 anesthesia resulted in reversible inhibition. ebony and silver mutants, which affect different pathways in catecholamine metabolism, showed an absent to reduced response. Using UVC lasers, we were able to demonstrate a response in different regions of the larval body. Furthermore, a 193 nm laser that penetrates only 2-5 mu m was able to induce a response but unable to kill the larvae. These results suggest a photochemical reaction occurs in the cuticle which produces free radicals that stimulate the nerves and muscle which are present immediately below the epidermis. Possible targets for the UVC radiation are catecholic compounds secreted and processed into the cuticle of third instar larvae just prior to pupariation whose primary function is to crosslink the protein and carbohydrate components. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CUTANEOUS BIOL RES CTR,DEPT DERMATOL,BOSTON,MA 02115. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,WELLMAN LABS PHOTOMED,BOSTON,MA 02115. KANSAS STATE UNIV,DEPT ENTOMOL,MANHATTAN,KS 66506. RI Raftery, Laurel/H-1406-2012 OI Raftery, Laurel/0000-0003-4797-4163 NR 26 TC 12 Z9 14 U1 0 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0739-4462 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PY 1996 VL 32 IS 2 BP 187 EP 196 DI 10.1002/(SICI)1520-6327(1996)32:2<187::AID-ARCH3>3.0.CO;2-W PG 10 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA UL911 UT WOS:A1996UL91100003 PM 8785418 ER PT J AU Adamis, AP Shima, DT Tolentino, MJ Gragoudas, ES Ferrara, N Folkman, J DAmore, PA Miller, JW AF Adamis, AP Shima, DT Tolentino, MJ Gragoudas, ES Ferrara, N Folkman, J DAmore, PA Miller, JW TI Inhibition of vascular endothelial growth factor prevents retinal ischemia-associated iris neovascularization in a nonhuman primate SO ARCHIVES OF OPHTHALMOLOGY LA English DT Article ID VEIN OCCLUSION; ANGIOGENESIS; MITOGEN; INVIVO; CELLS AB Objective: To determine if the angiogenic peptide vascular endothelial growth factor (VEGF) is required for retinal ischemia-associated iris neovascularization in a nonhuman primate. Methods: Laser retinal vein occlusion was used to produce retinal ischemia in 16 eyes of eight animals (Macaca fascicularis). Eyes were randomized to treatment every other day with intravitreal injections of either a neutralizing anti-VEGF monoclonal antibody or a control monoclonal antibody of the same isotype. Serial iris fluorescein angiograms were assessed using a standardized grading system and masked readers. Retinal VEGF and placental growth factor expression were assessed by Northern blotting. The specificity of the antibodies was determined in capillary endothelial cell proliferation assays prior to intravitreal injection. Results: Zero of eight eyes receiving the neutralizing anti-VEGF antibodies developed iris neovascularization. Five of eight control antibody-treated eyes developed iris neovascularization. The difference was statistically significant (P=.03). Intravitreal antibody injection did not impair the ability of the ischemic retina to increase VEGF messenger RNA expression. The anti-VEGF antibodies specifically inhibited VEGF-driven capillary endothelial cell proliferation in vitro. Conclusion: These data demonstrate that VEGF is required for iris neovascularization in an adult nonhuman primate eye. The inhibition of VEGF is a new potential therapeutic strategy for the treatment of ocular neovascularization. C1 HARVARD UNIV,CHILDRENS HOSP,SCH MED,DEPT SURG,LAB SURG RES,BOSTON,MA 02115. HARVARD UNIV,SCH MED,PROGRAM CELL & DEV BIOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. GENENTECH INC,DEPT CARDIOVASC RES,S SAN FRANCISCO,CA 94080. RP Adamis, AP (reprint author), MASSACHUSETTS EYE & EAR INFIRM,DEPT OPHTHALMOL,243 CHARLES ST,BOSTON,MA 02114, USA. NR 35 TC 434 Z9 457 U1 3 U2 8 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 SN 0003-9950 J9 ARCH OPHTHALMOL-CHIC JI Arch. Ophthalmol. PD JAN PY 1996 VL 114 IS 1 BP 66 EP 71 PG 6 WC Ophthalmology SC Ophthalmology GA TP701 UT WOS:A1996TP70100010 PM 8540853 ER PT S AU Sager, R Sheng, S Pemberton, P Hendrix, MJC AF Sager, R Sheng, S Pemberton, P Hendrix, MJC BE Gunthert, U Birchmeier, W TI Maspin: A tumor suppressing serpin SO ATTEMPTS TO UNDERSTAND METASTASIS FORMATION I: METASTASIS-RELATED MOLECULES SE Current Topics in Microbiology and Immunology LA English DT Review ID SERINE PROTEINASE-INHIBITOR; BREAST-CANCER C1 LXR BIOTECHNOL, RICHMOND, CA 94804 USA. ST LOUIS UNIV, SCH MED, CARDINAL GLENNON CHILDRENS HOSP, PEDIAT RES INST, ST LOUIS, MO 63110 USA. RP Sager, R (reprint author), DANA FARBER CANC INST, DEPT CANC GENET, 44 BINNEY ST, BOSTON, MA 02115 USA. FU NCI NIH HHS [CA61253] NR 14 TC 64 Z9 64 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN PA HEIDELBERGER PLATZ 3, D-14197 BERLIN, GERMANY SN 0070-217X BN 3-540-60680-7 J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 213 BP 51 EP 64 PN I PG 14 WC Immunology SC Immunology GA BJ66G UT WOS:A1996BJ66G00004 PM 8814994 ER PT J AU Brown, EM Segre, GV Goldring, SR AF Brown, EM Segre, GV Goldring, SR TI Serpentine receptors for parathyroid hormone, calcitonin and extracellular calcium ions SO BAILLIERES CLINICAL ENDOCRINOLOGY AND METABOLISM LA English DT Review ID OSTEOBLAST-LIKE CELLS; ROS 17/2.8 CELLS; TUMOR-NECROSIS-FACTOR; INDUCED HOMOLOGOUS DESENSITIZATION; FAMILIAL BENIGN HYPERCALCEMIA; NUCLEOTIDE REGULATORY PROTEIN; VASOACTIVE-INTESTINAL-PEPTIDE; CENTRAL-NERVOUS-SYSTEM; GENE-RELATED PEPTIDE; ADENYLATE-CYCLASE C1 MASSACHUSETTS GEN HOSP, ENDOCRINE UNIT, BOSTON, MA 02114 USA. DEACONESS HOSP, BOSTON, MA 02115 USA. RP Brown, EM (reprint author), BRIGHAM & WOMENS HOSP, DIV ENDOCRINE HYPERTENS, 221 LONGWOOD AVE, BOSTON, MA 02115 USA. FU NIDDK NIH HHS [DK44588, DK41415, DK48330] NR 174 TC 20 Z9 22 U1 0 U2 3 PU BAILLIERE TINDALL PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0950-351X J9 BAILLIERE CLIN ENDOC JI Baillieres Clin. Endocrinol. Metab. PD JAN PY 1996 VL 10 IS 1 BP 123 EP 161 DI 10.1016/S0950-351X(96)80346-6 PG 39 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TW947 UT WOS:A1996TW94700008 PM 8734454 ER PT J AU Ball, SG Baer, L Otto, MW AF Ball, SG Baer, L Otto, MW TI Symptom subtypes of obsessive-compulsive disorder in behavioral treatment studies: A quantitative review SO BEHAVIOUR RESEARCH AND THERAPY LA English DT Note ID THOUGHT-STOPPING TECHNIQUE; RESPONSE PREVENTION; EXPOSURE TREATMENT; THERAPY; NEUROSIS; RITUALS; RUMINATIONS; HABITUATION; CHECKING; PATIENT AB Recent reviews and meta-analytic studies have provided an encouraging account of the effectiveness of behavioral interventions for obsessive-compulsive disorder (OCD). One question regarding these estimates concerns their degree of generalizability to the range of OCD subtypes encountered in clinical settings. The purpose of the present study was to provide a quantitative description of the prevalence of various OCD subtypes (i.e. type of compulsions) within the behavioral treatment literature. We examined 65 studies that permitted classification of patients according to symptom subtype. Patients with primarily cleaning and/or checking compulsions predominated, accounting for 75% of the treatment population. On the other hand, patients with multiple compulsions or other compulsions, such as exactness, counting, hoarding, or slowness rituals were underrepresented, comprising only 12% of the population, which is markedly less than clinical epidemiological estimates. Rates of improvements in patients with OCD are most applicable to patients with cleaning and checking compulsions, but may not yet be generalizable to patients with other symptoms. These findings encourage studies of the efficacy of existing and novel interventions for patients with counting, repeating, symmetry, hoarding, or multiple compulsions in order to broaden the clinical application of OCD behavioral treatment. C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02115. RP Ball, SG (reprint author), INDIANA UNIV,SCH MED,541 CLIN DR,CLIN BLDG 291,INDIANAPOLIS,IN 46202, USA. NR 74 TC 98 Z9 99 U1 1 U2 14 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0005-7967 J9 BEHAV RES THER JI Behav. Res. Ther. PD JAN PY 1996 VL 34 IS 1 BP 47 EP 51 DI 10.1016/0005-7967(95)00047-2 PG 5 WC Psychology, Clinical SC Psychology GA TK219 UT WOS:A1996TK21900007 PM 8561764 ER PT J AU Kowluru, A Seavey, SE Rhodes, CJ Metz, SA AF Kowluru, A Seavey, SE Rhodes, CJ Metz, SA TI A novel regulatory mechanism for trimeric GTP-binding proteins in the membrane and secretory granule fractions or human and rodent beta cells SO BIOCHEMICAL JOURNAL LA English DT Article ID NUCLEOSIDE DIPHOSPHATE KINASE; INSULIN-SECRETION; RAT ISLETS; GAMMA-SUBUNITS; PHOSPHORYLATION; QUANTITATION; INHIBITORS; CONVERSION; ACTIVATION; CA-2+ AB Recently we described roles for heterotrimeric and low-molecular-mass GTP-binding proteins in insulin release from normal rat islets. During these studies, we observed that a protein with an apparent molecular mass (37 kDa) similar to that of the beta subunit of trimeric GTP-binding proteins underwent phosphorylation in each of five classes of insulin-secreting cells. Incubation of the beta cell total membrane fraction or the isolated secretory granule fraction (but not the cytosolic fraction) with [gamma-P-32]ATP or [gamma-P-32]GTP resulted in the phosphorylation of this protein, which was selectively immunoprecipitated by an antiserum directed against the common beta subunit of trimeric G-proteins. Disruption of the alpha beta gamma trimer (by pretreatment with either fluoroaluminate or guanosine 5'-[gamma-thio]triphosphate) prevented beta subunit phosphorylation. Based on differential sensitivities to pH, heat and the histidine-selective reagent diethyl pyrocarbonate (and reversal of the latter by hydroxylamine), the phosphorylated amino acid was presumptively identified as histidine. Incubation of pure beta subunit alone or in combination with the exogenous purified alpha subunit of transducin did not result in the phosphorylation of the beta subunit, but addition of the islet cell membrane fraction did support this event, suggesting that membrane localization (or a membrane-associated factor) is required for beta subunit phosphorylation. Incubation of phosphorylated beta subunit with G(alpha.GDP) accelerated the dephosphorylation of the beta subunit, accompanied by the formation of G(alpha.GTP). Immunoblotting detected multiple alpha subunits (of G(i), G(0) and G(q) and at least one beta subunit in the secretory granule fraction of normal rat islets and insulinoma cells. These data describe a potential alternative mechanism for the activation of GTP-binding proteins in beta cells which contrasts with the classical receptor-agonist mechanism: G(beta) undergoes transient phosphorylation at a histidine residue by a GTP-specific protein kinase; this phosphate, in turn, maybe transferred via a classical Ping-Pong mechanism to G(alpha.GDP) (inactive), yielding the active configuration G(alpha.GTP) in secretory granules (a strategic location to modulate exocytosis). C1 UNIV WISCONSIN, SCH MED, DEPT MED, MADISON, WI 53706 USA. UNIV WISCONSIN, SCH MED, ENDOCRINOL SECT, MADISON, WI 53706 USA. WILLIAM S MIDDLETON MEM VET ADM MED CTR, MADISON, WI 53705 USA. BRIGHAM & WOMENS HOSP, JOSLIN DIABET CTR, EP JOSLIN RES LAB, BOSTON, MA 02215 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02215 USA. FU NIDDK NIH HHS [DK37312, DK47919] NR 50 TC 80 Z9 83 U1 0 U2 1 PU PORTLAND PRESS LTD PI LONDON PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND SN 0264-6021 EI 1470-8728 J9 BIOCHEM J JI Biochem. J. PD JAN 1 PY 1996 VL 313 BP 97 EP 107 PN 1 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TN890 UT WOS:A1996TN89000013 PM 8546716 ER PT J AU Schuppin, GT Rhodes, CJ AF Schuppin, GT Rhodes, CJ TI Specific co-ordinated regulation of PC3 and PC2 gene expression with that of preproinsulin in insulin-producing beta TC3 cells SO BIOCHEMICAL JOURNAL LA English DT Article ID MESSENGER-RNA; TRANSLATIONAL CONTROL; PANCREATIC-ISLETS; BINDING PROTEIN; GLUCOSE; TRANSCRIPTION; BIOSYNTHESIS; CONVERTASE; LINE; LANGERHANS AB Short-term (less than 2 h) glucose stimulation of isolated pancreatic islets specifically increases the biosynthesis of proinsulin and its converting enzymes PC2 and PC3 at the translation level. To determine whether gene expression of PC2 and PC3 was also regulated by longer-term (more than 6 h) glucose stimulation along with that of preproinsulin, studies were performed with the beta TC3 insulin-producing cell line. By Northern blot analysis, glucose maintained PC2 and PC3 mRNA levels in parallel with those of preproinsulin. After 48 h, mRNA levels of preproinsulin, PC2 and PC3 were, respectively, 2.9 (P < 0.05), 3.0 (P < 0.005) and 5.3 (P < 0.001) times greater in the presence of glucose than in beta TC3 cells cultured in the absence of glucose. Glucose-regulated PC2 and PC3 gene expression, like that of preproinsulin, was maximal at glucose concentrations above 5.5 mM. Studies of mRNA stability showed that the half-lives of PC2 (9 h) and PC3 (5 h) mRNA were much shorter than that of preproinsulin mRNA (over 24 h), but little effect of glucose on stability of these mRNAs was observed. Nuclear run-off analysis indicated that transcription of preproinsulin, PC2 and PC3 was modestly induced after 1 h exposure to 16.7 mM glucose. Therefore preproinsulin, PC2 and PC3 mRNA levels in beta TC3 cells were most probably maintained at the level of gene transcription. In contrast, elevation of cyclic AMP by forskolin had no effect on mRNA levels or gene transcription of preproinsulin, PC2 and PC3, despite a cyclic-AMP-induced phosphorylation of the cyclic AMP response element binding protein that correlated with a marked increase in cJun and cFos gene transcription in the same beta-cells. These results suggest that preproinsulin, PC2 and PC3 gene transcription can be specifically glucose-regulated in a mechanism that is unlikely to involve a key role for cyclic AMP. The co-ordinate increase in PC2 and PC3 mRNA levels with that of preproinsulin mRNA in response to chronic glucose represents a long-term means of catering for an increased demand on proinsulin conversion. C1 BRIGHAM & WOMENS HOSP,JOSLIN DIABET CTR,EP JOSLIN RES LAB,BOSTON,MA 02215. HARVARD UNIV,SCH MED,BOSTON,MA 02215. FU NIDDK NIH HHS [DK 307260-18, DK 36836-09] NR 52 TC 35 Z9 36 U1 0 U2 0 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON, ENGLAND W1N 3AJ SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD JAN 1 PY 1996 VL 313 BP 259 EP 268 PN 1 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA TN890 UT WOS:A1996TN89000035 PM 8546693 ER PT J AU Pozsgay, V Trinh, L Shiloach, J Robbins, JB DonohueRolfe, A Calderwood, SB AF Pozsgay, V Trinh, L Shiloach, J Robbins, JB DonohueRolfe, A Calderwood, SB TI Purification of subunit B of Shiga toxin using a synthetic trisaccharide-based affinity matrix SO BIOCONJUGATE CHEMISTRY LA English DT Article ID SHIGELLA-DYSENTERIAE TYPE-1; CELL-SURFACE GLYCANS; TRI-SACCHARIDE; OLIGOSACCHARIDES; DERIVATIVES; GLYCOSIDES; DETERMINANT; ANTIGEN; POLYSACCHARIDE; RECEPTOR AB The blood group P-1 antigenic trisaccharide (3), which is the receptor-binding ligand of Shiga-like toxins, is synthesized in a spacer-equipped form (32) from 2-(trimethylsilyl)ethyl glucoside 5 and the 1-thiogalactoside building blocks 10 and 22 in a stereocontrolled, stepwise fashion. Covalent attachment of 32 to hydrazine group-containing agarose gel by reductive amination provided the P-1 trisaccharide-containing affinity sorbent which was used for preparative scale isolation of subunit B of Shiga toxin. C1 NIDDKD,CELLULAR & MOLEC BIOL LAB,BETHESDA,MD 20892. TUFTS UNIV,SCH VET MED,DEPT COMPARAT MED,N GRAFTON,MA 01536. MASSACHUSETTS GEN HOSP,INFECT DIS UNIT,BOSTON,MA 02114. RP Pozsgay, V (reprint author), NICHHD,DEV & MOLEC IMMUN LAB,BETHESDA,MD 20892, USA. NR 47 TC 12 Z9 12 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 1043-1802 J9 BIOCONJUGATE CHEM JI Bioconjugate Chem. PD JAN-FEB PY 1996 VL 7 IS 1 BP 45 EP 55 DI 10.1021/bc9500711 PG 11 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic SC Biochemistry & Molecular Biology; Chemistry GA TT950 UT WOS:A1996TT95000009 PM 8741990 ER PT J AU Bogdanov, AA Martin, C Bogdanova, AV Brady, TJ Weissleder, R AF Bogdanov, AA Martin, C Bogdanova, AV Brady, TJ Weissleder, R TI An adduct of cis-diamminedichloroplatinum (II) and poly(ethylene glycol)poly(L-lysine)-succinate: Synthesis and cytotoxic properties SO BIOCONJUGATE CHEMISTRY LA English DT Article ID POTENTIAL ANTITUMOR AGENTS; CARBOXYMETHYL-DEXTRAN; ANTICANCER DRUG; PLATINUM; CISPLATIN; COMPLEXES; TOXICITY; CARCINOMA; DELIVERY; SYSTEM AB A noncovalent adduct of the antineoplastic drug cis-diamminedichloroplatinum (cDDP) and a biocompatible graft copolymer of poly(L-lysine) and methylpoly(ethylene glycol) succinate is described. Upon incubation of cDDP with [O-methylpoly(ethylene glycol)-O'-succinyl]-N-epsilon-poly(L-lysine)(n)-N-epsilon-succinate, n = 250-270, highly soluble, long circulating adducts were formed which contained 4.3% of platinum by weight. Approximately 60% of the polymer-associated drug was released during dialysis against saline or serum albumin containing saline, with a half-time of release of 63 h. The adducts showed a pronounced antineoplastic effect in BT-20 human adenocarcinoma cell cultures. In cell proliferation assays, the concentration of half-inhibition of [H-3]thymidine uptake was 0.9 +/- 0.2 mu M for the drug-copolymer adduct compared to 0.3 +/- 0.1 mu M for free cDDP. The adduct showed a long blood half-life (ca. 14 h in rats) and accumulated in experimental mammary adenocarcinomas at 2.5-3.5% injected dose per gram of tissue. A central adduct of cDDP with the backbone portion of the copolymer, poly(L-lysine)-N-epsilon-succinate, had a short half-life in the bloodstream (ca. 30 min) and low accumulation (0.5% injected dose per gram) in tumor. A dual therapeutical effect of methylpoly((ethylene glycol)succinylpoly(L-lysine)-succinate as a carrier of cDDP is suggested: (1) as a earlier for systemic release of the active drug from the macromolecule while it circulates in the bloodstream and (2) as a carrier for on-site delivery which results from the release of the drug in the tumor as a consequence of accumulation of the copolymer in the tumor. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02129. RP Bogdanov, AA (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIOL,NMR CTR,MGH,MAGNET RESONANCE PHARMACEUT PROGRAM,ROOM 5416,BOSTON,MA 02129, USA. NR 32 TC 51 Z9 53 U1 0 U2 9 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 SN 1043-1802 J9 BIOCONJUGATE CHEM JI Bioconjugate Chem. PD JAN-FEB PY 1996 VL 7 IS 1 BP 144 EP 149 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Multidisciplinary; Chemistry, Organic SC Biochemistry & Molecular Biology; Chemistry GA TT950 UT WOS:A1996TT95000022 PM 8742003 ER PT B AU Hoop, B Burton, MD Kazemi, H AF Hoop, B Burton, MD Kazemi, H BE Khoo, MCK TI Fractal noise in breathing SO BIOENGINEERING APPROACHES TO PULMONARY PHYSIOLOGY AND MEDICINE LA English DT Proceedings Paper CT 12th Biomedical Simulations Resource Short Course on Bioengineering Approaches to Pulmonary Physiology and Medicine CY MAY 20, 1995 CL SEATTLE, WA C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,MED SERV,PULM & CRIT CARE UNIT,BOSTON,MA 02114. NR 0 TC 3 Z9 3 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45370-3 PY 1996 BP 161 EP 173 DI 10.1007/978-0-585-34964-0_10 PG 13 WC Engineering, Biomedical SC Engineering GA BG08M UT WOS:A1996BG08M00010 ER PT J AU Schmidt, EV AF Schmidt, EV TI Happenstance, circumstance or enemy action: Cyclin D1 in breast, eye and brain SO BIOESSAYS LA English DT Article ID CELL-CYCLE; SACCHAROMYCES-CEREVISIAE; MATING-PHEROMONE; G1 PHASE; GENE; IDENTIFICATION; AMPLIFICATION; EXPRESSION; ARREST; CANCER AB Two recent reports of mice homozygously deleted for cyclin D1 provide unequivocal evidence that the critical G(1) cyclin, cyclin D1, is by itself rate-limiting for growth in some mammalian tissues((1,2)). Cyclin D1 knockout mice are small and exhibit behavioral abnormalities. Specific hypoplasias of retinal and mammary tissues suggest an unusual dependence on cyclin D1 function for tissue growth in those organs. The odd coincidences that cyclin D1 functions as the retinoblastoma gene kinase, together with associations between increased cyclin D1 expression and breast cancer, suggest, but do not prove, a special function of cyclin D1 in those tissues. C1 MASSACHUSETTS GEN HOSP,CHILDRENS SERV,BOSTON,MA 02129. RP Schmidt, EV (reprint author), MASSACHUSETTS GEN HOSP,MGH CANC CTR,BLDG 149,13TH ST,BOSTON,MA 02129, USA. NR 29 TC 4 Z9 4 U1 0 U2 0 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE, CAMBS, ENGLAND CB4 4DL SN 0265-9247 J9 BIOESSAYS JI Bioessays PD JAN PY 1996 VL 18 IS 1 BP 6 EP 8 DI 10.1002/bies.950180104 PG 3 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA TT377 UT WOS:A1996TT37700003 PM 8593165 ER PT B AU Husain, D Miller, JW Kramer, M Gragoudas, ES Michaud, N Flotte, TJ AF Husain, D Miller, JW Kramer, M Gragoudas, ES Michaud, N Flotte, TJ BE Holick, MF Jung, EG TI Photodynamic therapy for the treatment of age related macular degeneration SO BIOLOGIC EFFECTS OF LIGHT 1995 LA English DT Proceedings Paper CT 4th International Arnold Rikli Symposium on Biologic Effects of Light CY OCT 09-11, 1995 CL ATLANTA, GA SP Inst Friedrich Wolff AG, Light Symp Fdn RP Husain, D (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA 02114, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WALTER DE GRUYTER PI BERLIN 30 PA GENTHINER STRASSE 13, W-1000 BERLIN 30, GERMANY BN 3-11-014899-4 PY 1996 BP 204 EP 210 PG 7 WC Biophysics; Dermatology SC Biophysics; Dermatology GA BH54R UT WOS:A1996BH54R00034 ER PT S AU Huang, PL Huang, ZH Moskowitz, MA Bevan, JA Fishman, MC AF Huang, PL Huang, ZH Moskowitz, MA Bevan, JA Fishman, MC BE Moncada, S Stamler, J Gross, S Higgs, EA TI Targeted disruption of the endothelial nitric oxide synthase gene SO BIOLOGY OF NITRIC OXIDE, PT 5 SE PORTLAND PRESS PROCEEDINGS LA English DT Proceedings Paper CT 4th International Meeting on the Biology of Nitric Oxide CY SEP, 1995 CL AMELIA ISL, FL C1 MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,BOSTON,MA 02129. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PORTLAND PRESS LTD PI LONDON PA 59 PORTLAND PL, LONDON, ENGLAND W1N 3AJ SN 0966-4068 BN 1-85578-102-6 J9 PORTL PR P PY 1996 VL 10 BP 10 EP 10 PG 1 WC Biochemistry & Molecular Biology; Cell Biology; Chemistry, Medicinal; Pharmacology & Pharmacy; Physiology SC Biochemistry & Molecular Biology; Cell Biology; Pharmacology & Pharmacy; Physiology GA BE95M UT WOS:A1996BE95M00010 ER PT S AU Maynard, KI Arango, PM Ogilvy, CS AF Maynard, KI Arango, PM Ogilvy, CS BE Moncada, S Stamler, J Gross, S Higgs, EA TI L(+)-amino-4-phosphonobutyric acid prevents nitric oxide-induced functional inhibition in the rabbit retina SO BIOLOGY OF NITRIC OXIDE, PT 5 SE PORTLAND PRESS PROCEEDINGS LA English DT Proceedings Paper CT 4th International Meeting on the Biology of Nitric Oxide CY SEP, 1995 CL AMELIA ISL, FL C1 MASSACHUSETTS GEN HOSP,NEUROSURG SERV,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PORTLAND PRESS LTD PI LONDON PA 59 PORTLAND PL, LONDON, ENGLAND W1N 3AJ SN 0966-4068 BN 1-85578-102-6 J9 PORTL PR P PY 1996 VL 10 BP 300 EP 300 PG 1 WC Biochemistry & Molecular Biology; Cell Biology; Chemistry, Medicinal; Pharmacology & Pharmacy; Physiology SC Biochemistry & Molecular Biology; Cell Biology; Pharmacology & Pharmacy; Physiology GA BE95M UT WOS:A1996BE95M00295 ER PT J AU Flaws, JA Hirshfield, AN Tilly, JL DeSanti, AM Davis, MA AF Flaws, JA Hirshfield, AN Tilly, JL DeSanti, AM Davis, MA TI Activation of mitogen activated protein kinases during follicular atresia and survival. SO BIOLOGY OF REPRODUCTION LA English DT Meeting Abstract C1 UMAB,SCH MED,DEPT ANAT,BALTIMORE,MD. UMAB,SCH MED,DEPT PATHOL,BALTIMORE,MD. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT OBSTET & GYNECOL,VINCENT CTR REPROD BIOL,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC STUDY REPRODUCTION PI MADISON PA 1603 MONROE ST, MADISON, WI 53711-2021 SN 0006-3363 J9 BIOL REPROD JI Biol. Reprod. PY 1996 VL 54 SU 1 BP 120 EP 120 PG 1 WC Reproductive Biology SC Reproductive Biology GA VF099 UT WOS:A1996VF09900125 ER PT J AU Tilly, JL Flaws, JA DeSanti, AM Hughes, FM Tilly, KI Maravei, DV Trbovich, AM Cidlowski, JA Hirshfield, AN AF Tilly, JL Flaws, JA DeSanti, AM Hughes, FM Tilly, KI Maravei, DV Trbovich, AM Cidlowski, JA Hirshfield, AN TI Biochemical and morphological investigation of apoptosis and atresia in rat ovarian antral follicles incubated in vitro. SO BIOLOGY OF REPRODUCTION LA English DT Meeting Abstract C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT OBSTET & GYNECOL,VINCENT CTR REPROD BIOL,BOSTON,MA. UNIV MARYLAND,SCH MED,DEPT ANAT,BALTIMORE,MD 21201. NIEHS,LAB INTEGRAT BIOL,RES TRIANGLE PK,NC 27709. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SOC STUDY REPRODUCTION PI MADISON PA 1603 MONROE ST, MADISON, WI 53711-2021 SN 0006-3363 J9 BIOL REPROD JI Biol. Reprod. PY 1996 VL 54 SU 1 BP 124 EP 124 PG 1 WC Reproductive Biology SC Reproductive Biology GA VF099 UT WOS:A1996VF09900129 ER PT J AU Schultze, JL Gribben, JG AF Schultze, JL Gribben, JG TI Minimal residual disease in non-Hodgkin's lymphoma SO BIOMEDICINE & PHARMACOTHERAPY LA English DT Article DE minimal residual disease; non-Hodgkin's lymphoma; PCR; clinical assessment ID POLYMERASE CHAIN-REACTION; BONE-MARROW TRANSPLANTATION; B-CELL LYMPHOMA; BREAKPOINT-CLUSTER REGION; FOLLICULAR LYMPHOMA; PERIPHERAL-BLOOD; CHROMOSOME-TRANSLOCATION; GENE; T(14-18); BCL-2 AB Recent advances in the sensitivity of detection methods have clearly illustrated that patients in complete clinical remission often harbor residual lymphoma cells that are below the limit of detection using standard staging techniques. However, the clinical significance of this detection is by no means clear. Patients have been identified who remain in very long-term clinical remission despite detection of residual lymphoma cells. In contrast, the eradication of lymphoma is associated with improved outcome in patients undergoing autologous bone marrow transplantation. We review the methodologies for the detection of minimal residual lymphoma and discusses the clinical significance of this detection. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEPT MED,DIV HEMATOL MALIGNANCIES,BOSTON,MA 02115. RI Schultze, Joachim/D-7794-2011 OI Schultze, Joachim/0000-0003-2812-9853 NR 36 TC 4 Z9 4 U1 0 U2 0 PU EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 141 RUE JAVEL, 75747 PARIS, FRANCE SN 0753-3322 J9 BIOMED PHARMACOTHER JI Biomed. Pharmacother. PY 1996 VL 50 IS 9 BP 451 EP 458 DI 10.1016/S0753-3322(97)86005-2 PG 8 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA VX578 UT WOS:A1996VX57800005 PM 8991117 ER PT J AU Lazarus, HM Elias, AD AF Lazarus, HM Elias, AD TI Autologous bone marrow and peripheral blood progenitor cell transplants in small cell lung cancer SO BONE MARROW TRANSPLANTATION LA English DT Editorial Material ID HIGH-DOSE CHEMOTHERAPY; COMBINED MODALITY THERAPY; PHASE-I; INTENSIFICATION THERAPY; SOLID TUMORS; BRONCHOGENIC-CARCINOMA; CYCLOPHOSPHAMIDE; ETOPOSIDE; VP-16; CISPLATIN C1 UNIV HARTFORD,SCH MED,DANA FARBER CANC INST,BOSTON,MA. RP Lazarus, HM (reprint author), CASE WESTERN RESERVE UNIV,DEPT MED,IRELAND CANC CTR,UNIV CLEVELAND HOSP,11100 EUCLID AVE,CLEVELAND,OH 44106, USA. NR 46 TC 9 Z9 9 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0268-3369 J9 BONE MARROW TRANSPL JI Bone Marrow Transplant. PD JAN PY 1996 VL 17 IS 1 BP 1 EP 3 PG 3 WC Biophysics; Oncology; Hematology; Immunology; Transplantation SC Biophysics; Oncology; Hematology; Immunology; Transplantation GA TR014 UT WOS:A1996TR01400001 PM 8673040 ER PT J AU Karpati, G Brown, RH AF Karpati, G Brown, RH TI The dawning of a new era in the molecular biology of the muscular dystrophies SO BRAIN PATHOLOGY LA English DT Editorial Material ID DUCHENNE; PROTEIN; GENE C1 MASSACHUSETTS GEN HOSP,CECIL B DAY LAB NEUROMUSCULAR DIS NEUROL,BOSTON,MA 02129. RP Karpati, G (reprint author), MONTREAL NEUROL INST,NEUROMUSCULAR RES GRP,RM 633,3801 UNIV ST,MONTREAL,PQ H3A 2B4,CANADA. NR 8 TC 3 Z9 3 U1 0 U2 0 PU INT SOC NEUROPATHOLOGY PI ZURICH PA ISN JOURNAL PO BOX, CH-8033 ZURICH, SWITZERLAND SN 1015-6305 J9 BRAIN PATHOL JI Brain Pathol. PD JAN PY 1996 VL 6 IS 1 BP 17 EP 17 DI 10.1111/j.1750-3639.1996.tb00778.x PG 1 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA TV413 UT WOS:A1996TV41300004 ER PT J AU Sibonga, JD Evans, GL Hauck, ER Bell, NH Turner, RT AF Sibonga, JD Evans, GL Hauck, ER Bell, NH Turner, RT TI Ovarian status influences the skeletal effects of tamoxifen in adult rats SO BREAST CANCER RESEARCH AND TREATMENT LA English DT Article DE antiestrogen; Tamoxifen; histomorphometry; ovary-intact rat; bone structure ID TERM ADJUVANT TAMOXIFEN; BONE-MINERAL DENSITY; BREAST-CANCER; OVARIECTOMIZED RATS; POSTMENOPAUSAL WOMEN; GROWING-RATS; FEMALE RATS; ESTROGEN; THERAPY; HISTOMORPHOMETRY AB Tamoxifen (TAM), an antiestrogen used in adjuvant therapy for breast cancer, is currently being evaluated for prevention of breast cancer in premenopausal and postmenopausal disease-free women. In light of this clinical application in young women, the skeleton's potential predisposition for osteoporosis following long-term treatment with an antiestrogen is a concern. In postmenopausal women being treated for breast cancer TAM was shown to prevent bone loss. There is little information, however, about the skeletal effects of TAM in premenopausal women. Previous animal studies in ovariectomized (OVX'd) rats have consistently reported TAM to prevent cancellous and cortical bone loss. The effects of TAM on ovary-intact animals, however, are not well established. We have performed a histomorphometric analysis in order to evaluate the influence of ovarian function on the skeletal effects of long-term TAM treatment in the laboratory animal model. Six-month-old rats were implanted subcutaneously with pellets designed for the controlled release of TAM at a dose (5 mg/3 wks) previously shown to be effective at antagonizing short-term bone loss in OVX'd growing rats. TAM acted as an estrogen agonist on cortical bone measurements in tibia of ovary-intact as well as OVX'd rats. In cancellous bone of OVX'd rats, TAM reduced indices of bone formation and resorption and reduced the bone loss from over 90 percent to less than 50 percent. In ovary-intact rats, however, TAM produced a 31 percent loss of cancellous bone, a deficit associated with a 26 percent reduction in the trabecular number. These results clearly demonstrate an interaction between TAM and ovarian status whereby TAM partially prevents estrogen-deficient bone loss in OVX'd animals but antagonizes selective actions of estrogen on the skeleton of ovary-intact animals. C1 MAYO CLIN,DEPT BIOCHEM & MOL BIOL,ROCHESTER,MN 55905. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,RES SERV,CHARLESTON,SC 29401. MAYO CLIN,DEPT ORTHOPED RES,ROCHESTER,MN 55905. FU NIAMS NIH HHS [AR 41418] NR 37 TC 17 Z9 17 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0167-6806 J9 BREAST CANCER RES TR JI Breast Cancer Res. Treat. PY 1996 VL 41 IS 1 BP 71 EP 79 DI 10.1007/BF01807038 PG 9 WC Oncology SC Oncology GA VR620 UT WOS:A1996VR62000008 PM 8932878 ER PT J AU Shimazaki, J Laing, RA Tsubota, K Kenyon, KR AF Shimazaki, J Laing, RA Tsubota, K Kenyon, KR TI Non-invasive assessment of the donor corneal endothelium using ocular redox fluorometry SO BRITISH JOURNAL OF OPHTHALMOLOGY LA English DT Article ID PYRIDINE-NUCLEOTIDE FLUORESCENCE; METABOLIC ANALYSIS AB Aims - To investigate the usefulness of ocular redox fluorometry for evaluating donor corneal endothelial viability. Methods - Corneas from 42 recipients of penetrating keratoplasty and four donor corneas were examined by ocular redox fluorometry. Autofluorescence from reduced pyridine nucleotides (PN) and oxidised flavoproteins (Fp) of the human corneal endothelium were measured noninvasively, and the PN/Fp ratio was used as a tissue metabolic indicator. Specular microscopy and electron microscopy were also performed. Results - Both the quality of specular microscopic image and the PN/Fp ratio were significantly correlated with the degree of corneal endothelial damage determined by histological examination. Corneas with poor specular microscopic image showed significantly decreased PN/Fp ratio compared with corneas with good or fair specular images (p=0.041 and 0.027, respectively). The PN/Fp ratio increased in corneas with mildly damaged endothelium but decreased in corneas with severely damaged endothelium determined by histological examination. Evaluation of corneal endothelium by combination of specular microscopy and ocular redox fluorometry showed excellent association with that of histopathological examination (p<0.0001). Conclusion - Ocular redox fluorometry is useful for assessing donor corneal endothelial viability. Combination of ocular redox fluorometry and specular microscopy may increase the ability of donor cornea selection. C1 BOSTON UNIV,SCH MED,DEPT OPHTHALMOL,BOSTON,MA 02118. MASSACHUSETTS EYE & EAR INFIRM,BOSTON,MA 02114. RP Shimazaki, J (reprint author), TOKYO DENT COLL,DEPT OPHTHALMOL,5-11-13 SUGANO,ICHIKAWA,CHIBA 272,JAPAN. NR 12 TC 6 Z9 6 U1 0 U2 0 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0007-1161 J9 BRIT J OPHTHALMOL JI Br. J. Ophthalmol. PD JAN PY 1996 VL 80 IS 1 BP 69 EP 73 DI 10.1136/bjo.80.1.69 PG 5 WC Ophthalmology SC Ophthalmology GA TP259 UT WOS:A1996TP25900017 PM 8664237 ER PT J AU Limmroth, V Lee, WS Moskowitz, MA AF Limmroth, V Lee, WS Moskowitz, MA TI GABA(A)-receptor-mediated effects of progesterone, its ring-A-reduced metabolites and synthetic neuroactive steroids on neurogenic oedema in the rat meninges SO BRITISH JOURNAL OF PHARMACOLOGY LA English DT Article DE GABA(A)-receptors; neurogenic inflammation; progesterone; neurosteroids; headache; migraine ID DURA-MATER; BLOOD-VESSELS; PLASMA; RECEPTOR; BRAIN; 3-ALPHA-HYDROXY-5-ALPHA-PREGNAN-20-ONE; EXTRAVASATION; STIMULATION; MODULATORS; MIGRAINE AB 1 The effects of progesterone, its A-ring-reduced metabolites, allopregnanolone, tetrahydroxydeoxycorticosterone and the synthetic neuroactive steroid alphaxalone were evaluated in a rat model of plasma extravasation within the meninges following unilateral electrical stimulation (ES) of the trigeminal ganglion (0.6 mA, 5 ms, 5 min) or substance P administration (1 nmol kg(-1), i.v.). 2 When administered 55 min prior to electrical stimulation, progesterone (greater than or equal to 500 mu g, s.c.) dose-dependently decreased plasma extravasation within the meninges (ED(50): 650 mu g) but not within conjunctiva and tongue. Promegestone (R5020), a non-metabolized progesterone agonist (1000 mu g, i.p.) was ineffective. The administration of progesterone (greater than or equal to 500 mu g s.c.) 55 min prior to substance P partially suppressed plasma extravasation within the meninges (ED(50): 550 mu g). 3 The GABA(A)-antagonist, bicuculline (ED(50): 8.2 mu g kg(-1), i.p) but not the GABA(B)-antagonist, phaclofen (100 mu g kg(-1) i.p.) attenuated the effects of progesterone after electrical stimulation and substance P administration. 4 The metabolites of progesterone, allopregnanolone (3 alpha-hydroxy-5 alpha-pregnan-20-one (THP); ED(50): 0.58 mu g kg(-1), i.p.), tetrahydroxydeoxycorticosterone (3 alpha,21-dihydroxy-5 alpha-pregnan-20-one (THDOC); ED(50): 1.2 mu g kg(-1), i.p.) as well as the synthetic steroid alphaxalone (3 alpha-hydroxy-5 alpha-pregnane-11,20-dione; ED(50): 1.8 mu g kg(-1), i.p.) suppressed plasma extravasation dose-dependently following ES, whereas the epimer of allopregnanolone, 3 beta-hydroxy-5 alpha-pregnan-20-one (100 mu g kg(-1), i.p.), did not. Extravasation caused by SP administration was partially suppressed by allopregnanolone (greater than or equal to 1 mu g kg(-1), i.p.) (ED(50):2.1 mu g kg(-1)). 5 The effect of progesterone (1000 mu g, s.c.) and allopregnanolone (100 mu g kg(-1), i.p.) on neurogenic plasma extravasation was reversed by bicuculline (10 mu g kg(-1), i.p.) or by a congener, bicuculline-methiodide (10 mu g kg(-1), i.p.) which does not cross the blood brain barrier. 6 Progesterone (1000 mu g, s.c.) had no effect on mean arterial blood pressure or heart rate when measured for 60 min after administration. 7 These results indicate that neurosteroid modulation of a GABA(A)-receptor located outside the blood brain barrier suppresses neurogenic and substance P-induced plasma extravasation within the meninges. The findings are consistent with previously reported data showing that valproic acid and muscimol inhibit meningeal oedema by bicuculline-sensitive mechanisms. Drugs which activate GABA(A)-receptors and its modulatory sites might be clinically effective in the treatment of migraine and cluster headache. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT NEUROL,DEPT NEUROSURG,BOSTON,MA 02114. RI Moskowitz, Michael/D-9916-2011 FU NINDS NIH HHS [NS21558] NR 34 TC 64 Z9 65 U1 0 U2 1 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0007-1188 J9 BRIT J PHARMACOL JI Br. J. Pharmacol. PD JAN PY 1996 VL 117 IS 1 BP 99 EP 104 PG 6 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TY674 UT WOS:A1996TY67400015 PM 8825349 ER PT J AU Weinstock, R Leong, GB Silva, JA AF Weinstock, R Leong, GB Silva, JA TI California's diminished capacity defense: Evolution and transformation SO BULLETIN OF THE AMERICAN ACADEMY OF PSYCHIATRY AND THE LAW LA English DT Article ID LIMITS; CRAZY AB Diminished capacity survives in California as a severely attenuated mens rea defense known as diminished actuality, Some other states have similar limited strict mens rea defenses, The lost advantages of California's former expanded concept of diminished capacity are reviewed, As opposed to the all-or-none insanity defense, mens rea defenses permit the trier of fact to find gradations of guilt but are generally inapplicable unless the elements of a crime are redefined to permit consideration of motivational aspects, as California had done, The change from diminished capacity to a diminished actuality defense was a return to the complex, somewhat artificial legal concept of intent and a resurrection of confusing and antiquated common law definitions, The change was made in response to an unpopular jury verdict and a political climate in which little interest existed or still exists for understanding the reasons behind the commission of any crime, Some of the later restrictions imposed by the California Supreme Court on allowing voluntary intoxication to reduce murder to voluntary manslaughter logically should not apply to mental illness, Knowledge of the complex mens rea issues and the various relevant current defenses is essential for any forensic psychiatrist evaluating defendants in jurisdictions in which such defenses are admissible. C1 UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. UNIV MISSOURI,COLUMBIA,MO 65211. HARRY S TRUMAN MEM VET HOSP,COLUMBIA,MO 65201. S TEXAS VET HLTH CARE SYST,SAN ANTONIO,TX. RP Weinstock, R (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,PSYCHIAT SERV 116AC,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 23 TC 8 Z9 8 U1 1 U2 1 PU AMER ACAD PSYCHIATRY LAW PI BLOOMFIELD PA ONE REGENCY DR, PO BOX 30, BLOOMFIELD, CT 06002 SN 0091-634X J9 B AM ACAD PSYCH LAW JI Bull. Amer. Acad. Psychiat. Law PY 1996 VL 24 IS 3 BP 347 EP 366 PG 20 WC Law; Psychiatry SC Government & Law; Psychiatry GA VK941 UT WOS:A1996VK94100005 PM 8889134 ER PT J AU Eth, S Leong, GB Garrick, TR AF Eth, S Leong, GB Garrick, TR TI Tales of the crypt for psychiatrists: Mourning, melancholia, and mortuary malpractice SO BULLETIN OF THE AMERICAN ACADEMY OF PSYCHIATRY AND THE LAW LA English DT Article ID BEREAVEMENT AB Death awaits all, leaving in its wake relatives and friends affected by the loss of a loved one. Immediately following death, the funeral process begins, resulting in permanent burial in a cemetery. This report investigates the dysfunctional interactions between grief-stricken relatives and mortuaries that are associated with civil litigation for negligence. Psychiatric evaluations of 25 bereaved plaintiffs from nine separate lawsuits were performed. In addition, medical records and legal pleadings were reviewed as sources of additional information. General themes from the clinical material are identified and illustrated by two cases. Surviving relatives are in an acute state of emotional turmoil, rendering them exquisitely sensitive to lapses in expected routine and perceived disrespect toward the deceased. These issues are intensified when the circumstances of the death were traumatic, when the relationship with the deceased was ambivalent, when specific cultural and religious factors are present, and when the influence of litigation is felt. If the burial process is disrupted, civil suits for negligence may be filed that exacerbate grief and challenge the psychiatrist's efforts to resolve diagnostic ambiguity in the face of emotionally charged cultural and religious practices. C1 NEW YORK MED COLL,DEPT PSYCHIAT,NEW YORK,NY. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. UNIV MISSOURI,SCH MED,DEPT PSYCHIAT & NEUROL,COLUMBIA,MO. HARRY S TRUMAN VA MED CTR,COLUMBIA,MO. RP Eth, S (reprint author), ST VINCENTS HOSP,DEPT PSYCHIAT,153 W 11TH ST,NEW YORK,NY 10011, USA. NR 16 TC 0 Z9 0 U1 1 U2 1 PU AMER ACAD PSYCHIATRY LAW PI BLOOMFIELD PA ONE REGENCY DR, PO BOX 30, BLOOMFIELD, CT 06002 SN 0091-634X J9 B AM ACAD PSYCH LAW JI Bull. Amer. Acad. Psychiat. Law PY 1996 VL 24 IS 4 BP 483 EP 492 PG 10 WC Law; Psychiatry SC Government & Law; Psychiatry GA WB050 UT WOS:A1996WB05000004 PM 9001746 ER PT S AU Li, XG Haluska, P Hsiang, YH Bharti, A Kufe, DW Rubin, EH AF Li, XG Haluska, P Hsiang, YH Bharti, A Kufe, DW Rubin, EH BE Pantazis, P Giovanella, BC Rothenberg, ML TI Identification of topoisomerase I mutations affecting both DNA cleavage and interaction with camptothecin SO CAMPTOTHECINS: FROM DISCOVERY TO THE PATIENT SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Conference on the Camptothecins - From Discovery to the Patient CY FEB 07-10, 1996 CL BETHESDA, MD SP New York Acad Sci, Pharmacia & Upjohn Inc, Janssen Pharm, NCI, Rhone Poulenc Rorer, SmithKline Beecham Pharm, RGK Fdn, Yakult Honsha Co Ltd, Boehringer Ingelheim KG, Daiichi Pharm Corp, Genentech Inc, Marion Merrell Dow Inc, Rhone Poulenc Rorer Fdn, Stehlin Fdn Canc Res ID SV40 DNA; POINT MUTATION; DUPLEX DNA; BINDING; RESISTANCE; MUTANT; DOMAIN; SITE; MECHANISM; COVALENT C1 UNIV MED & DENT NEW JERSEY, ROBERT WOOD JOHNSON MED SCH, DEPT PHARMACOL, PISCATAWAY, NJ 08854 USA. CANC INST NEW JERSEY, PISCATAWAY, NJ 08854 USA. DANA FARBER CANC INST, DIV CANC PHARMACOL, BOSTON, MA 02115 USA. FU NCI NIH HHS [CA70981] NR 37 TC 20 Z9 20 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-057-3 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 803 BP 111 EP 127 DI 10.1111/j.1749-6632.1996.tb26381.x PG 17 WC Oncology; Multidisciplinary Sciences; Pharmacology & Pharmacy SC Oncology; Science & Technology - Other Topics; Pharmacology & Pharmacy GA BH28S UT WOS:A1996BH28S00011 PM 8993505 ER PT S AU Eder, JP Rubin, E Stone, R Bryant, M Xu, GX Supko, J Kinchla, N Lynch, T Hurwitz, S Rodriguez, D Shapiro, C Toppmeyer, D Grossbard, M Vosburg, E Huberman, M Schnipper, L Shulman, L Kufe, DW AF Eder, JP Rubin, E Stone, R Bryant, M Xu, GX Supko, J Kinchla, N Lynch, T Hurwitz, S Rodriguez, D Shapiro, C Toppmeyer, D Grossbard, M Vosburg, E Huberman, M Schnipper, L Shulman, L Kufe, DW BE Pantazis, P Giovanella, BC Rothenberg, ML TI Trials of 9-amino-20(S)-camptothecin in Boston SO CAMPTOTHECINS: FROM DISCOVERY TO THE PATIENT SE Annals of the New York Academy of Sciences LA English DT Article; Proceedings Paper CT Conference on the Camptothecins - From Discovery to the Patient CY FEB 07-10, 1996 CL BETHESDA, MD SP New York Acad Sci, Pharmacia & Upjohn Inc, Janssen Pharm, NCI, Rhone Poulenc Rorer, SmithKline Beecham Pharm, RGK Fdn, Yakult Honsha Co Ltd, Boehringer Ingelheim KG, Daiichi Pharm Corp, Genentech Inc, Marion Merrell Dow Inc, Rhone Poulenc Rorer Fdn, Stehlin Fdn Canc Res ID CAMPTOTHECIN NSC-100880; TOPOISOMERASE-I; PHASE-I; 9-AMINOCAMPTOTHECIN; CANCER; RING C1 MASSACHUSETTS GEN HOSP, BOSTON, MA 02115 USA. UNIV BOSTON HOSP, BOSTON, MA 02115 USA. NEW ENGLAND DEACONESS HOSP, BOSTON, MA 02115 USA. BETH ISRAEL HOSP, BOSTON, MA 02115 USA. BRIGHAM & WOMENS HOSP, BOSTON, MA 02115 USA. RP Eder, JP (reprint author), DANA FARBER CANC INST, BOSTON, MA 02115 USA. NR 16 TC 8 Z9 8 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-057-3 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 803 BP 247 EP 255 DI 10.1111/j.1749-6632.1996.tb26394.x PG 9 WC Oncology; Multidisciplinary Sciences; Pharmacology & Pharmacy SC Oncology; Science & Technology - Other Topics; Pharmacology & Pharmacy GA BH28S UT WOS:A1996BH28S00024 PM 8993518 ER PT J AU Southern, JF Warshaw, AL Lewandrowski, KB AF Southern, JF Warshaw, AL Lewandrowski, KB TI DNA ploidy analysis of mucinous cystic tumors of the pancreas - Correlation of aneuploidy with malignancy and poor prognosis SO CANCER LA English DT Article DE pancreas; cystic tumor; ploidy; mucinous neoplasm ID FLOW-CYTOMETRY; IMAGE-ANALYSIS; BREAST-CANCER; CARCINOMAS AB BACKGROUND. Benign and malignant pancreatic mucinous tumors differ in proliferative activity, production of tumor markers, and expression of growth factors. DNA ploidy is a useful index of aggressiveness and poor survival in ductal adenocarcinoma, but has not been studied in pancreatic cystic tumors. METHODS. The DNA ploidy status of Fuelgen-stained tissue sections of pancreatic mucinous cystic tumors was evaluated by image cytometry and related to clinical outcome obtained by case record review. RESULTS. Ploidy status correlated with malignancy and poor clinical outcome. All benign mucinous cystadenomas (n = 13) were diploid and cured by resection. Patients with diploid cystadenocarcinomas (n = 6) had all 83% survival rate following resection, while patients with aneuploid cystadenocarcinomas (n = 5) all died (four of disease and the fifth of another cause). The difference in survival between the diploid and aneuploid carcinomas was significant (P = 0.04). CONCLUSIONS. DNA ploidy status of pancreatic mucinous tumors by image analysis provides quantifiable information that may be predictive of clinical outcome. DNA aneuploidy appears to be a significant differential factor in pancreatic mucinous tumors and is associated with Shortened survival in mucinous cystadenocarcinomas. (C) 1996 American Cancer Society. C1 MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. NR 18 TC 12 Z9 12 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD JAN 1 PY 1996 VL 77 IS 1 BP 58 EP 62 DI 10.1002/(SICI)1097-0142(19960101)77:1<58::AID-CNCR11>3.0.CO;2-7 PG 5 WC Oncology SC Oncology GA TL670 UT WOS:A1996TL67000011 PM 8630940 ER PT J AU Ozdemirli, M Mankin, HJ Aisenberg, AC Harris, NL AF Ozdemirli, M Mankin, HJ Aisenberg, AC Harris, NL TI Hodgkin's disease presenting as a solitary bone tumor - A report of four cases and review of the literature SO CANCER LA English DT Review DE Hodgkin's disease; bone; lymphoma ID MALIGNANT-LYMPHOMA; MARROW INVOLVEMENT; MANIFESTATIONS AB BACKGROUND. Hodgkin's disease (HD) rarely presents as a solitary bone tumor. Fewer than 20 such cases have been reported in the English literature; many of these were reported prior to the development of immunohistologic markers for HD and T- and B-cell lymphomas. In this report, we describe four cases of HD that presented as a localized solitary mass in bone; the diagnosis was confirmed by immunohistochemical studies in all cases. METHODS. The biopsy specimens of four cases identified in our files were studied by conventional histopathology and immunohistochemistry. Clinical data and follow-up information were obtained for all patients. RESULTS. Three cases presented as a localized, solitary mass in the ilium and one case in the vertebra (T12). Three patients were female and one male. The average age was 43 years. Three of the patients presented with lower back pain without constitutional symptoms. AU had solitary osteoblastic lesions. AU four cases were diagnostic problems, and the diagnosis was confirmed in each case only after finding lymph node involvement. Bone biopsies showed fibrosis and a mixed inflammatory infiltrate with rare atypical cells. All four patients were subsequently found to have nodal involvement by HD. The histology of the associated nodal disease was mixed cellularity in two cases and nodular sclerosis in two. On immunohistochemical staining, the neoplastic cells in all cases expressed CD15 and CD30 and lacked CD45 and other B- and T-cell antigens. Three patients who were treated for HD are alive and well, 1, 6, and 14 years later. CONCLUSIONS. Although rare, HD should be considered in the differential diagnosis of solitary bone lesions. Most patients who present with apparently solitary HD of bone prove to have nodal involvement. Long-term survival is possible with aggressive treatment. (C) 1996 American Cancer Society. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,JAMES HOMER WRIGHT PATHOL LABS,BOSTON,MA. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT ORTHOPED SURG,BOSTON,MA. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT MED,BOSTON,MA. NR 35 TC 30 Z9 30 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0008-543X J9 CANCER JI Cancer PD JAN 1 PY 1996 VL 77 IS 1 BP 79 EP 88 DI 10.1002/(SICI)1097-0142(19960101)77:1<79::AID-CNCR14>3.3.CO;2-V PG 10 WC Oncology SC Oncology GA TL670 UT WOS:A1996TL67000014 PM 8630944 ER PT J AU Shieh, HL Hansen, H Zhu, JW Riedel, H AF Shieh, HL Hansen, H Zhu, JW Riedel, H TI Activation of conventional mammalian protein kinase C isoforms expressed in budding yeast modulates the cell doubling time - Potential in vivo screen for protein kinase C activators SO CANCER DETECTION AND PREVENTION LA English DT Article DE bryostatin 5; ingenol dibenzoate; mezerein; octyl-indolactam V; phorbol ester tumor promoter; Saccharomyces cerevisiae ID PHORBOL ESTER BINDING; TUMOR PROMOTERS; ANTINEOPLASTIC AGENTS; BIOLOGICAL RESPONSES; SYNTHETIC ANALOGS; LIGAND REGULATION; TELEOCIDIN; RECEPTOR; DOMAIN; BOVINE AB Conventional mammalian protein kinase C (PKC) isoforms alpha, beta 1, and gamma were expressed in Saccharomyces cerevisiae and resulted in a differential increase in the yeast doubling time in response to distinct classes of PKC activators. Mutants were created in the regulatory domain of PKC alpha to map the interaction with the different activators. The macrocyclic lactone bryostatin 5 preferentially regulated PKC alpha activity through the second cysteine-rich sequence (CYS2) of C1, while regulation by the diterpene ester mezerein displayed strong preference for the first cysteine-rich sequence (CYS1) of C1. The phorbol esters phorbol-12-myristate-13-acetate (PMA) and 12-deoxyphorbol 13-phenylacetate 20-acetate (dPPA) regulated PKC enzymatic activity equally potently via CYS1 or CYS2 albeit at reduced levels compared with native PKC alpha. For the diterpene ester ingenol-3, 20-dibenzoate and the indol alkaloids (-)-7-octyl-indolactam V and (-)-indolactam V, no responses were observed for mutants lacking either CYS1 or CYS2 whereas native PKC alpha activity was regulated. These in vivo results were complemented by in vitro binding and catalytic assays which showed correlation between PKC enzymatic activity and the cell growth characteristics. The observed phenotype can be exploited to screen natural compounds in vivo for their PKC regulatory potential and to map the underlying interactions. C1 JOSLIN DIABET CTR, MOL BIOL SECT, BOSTON, MA 02215 USA. NR 74 TC 10 Z9 10 U1 0 U2 1 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0361-090X J9 CANCER DETECT PREV JI Cancer Detect. Prev. PY 1996 VL 20 IS 6 BP 576 EP 589 PG 14 WC Oncology SC Oncology GA VU771 UT WOS:A1996VU77100003 PM 8939343 ER PT B AU Dranoff, G Mulligan, RC AF Dranoff, G Mulligan, RC BE Mihich, E Housman, D TI Unexpected functions of granulocyte-macrophage colony stimulating factor SO CANCER GENES: FUNCTIONAL ASPECTS SE PEZCOLLER FOUNDATION SYMPOSIA LA English DT Proceedings Paper CT 7th Pezcoller Symposium on Cancer Genes - Functional Aspects CY JUN 14-16, 1995 CL TRENT, ITALY SP Pezcoller Fdn RP Dranoff, G (reprint author), DANA FARBER CANC INST,BOSTON,MA 02115, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 BN 0-306-45482-3 J9 PEZ FDN SYM PY 1996 VL 7 BP 269 EP 280 PG 12 WC Oncology; Genetics & Heredity SC Oncology; Genetics & Heredity GA BH02L UT WOS:A1996BH02L00017 ER PT J AU Freedman, AS AF Freedman, AS TI Cell surface antigens in leukemias and lymphomas SO CANCER INVESTIGATION LA English DT Review ID ACUTE LYMPHOBLASTIC-LEUKEMIA; CHRONIC LYMPHOCYTIC-LEUKEMIA; ACUTE MYELOID-LEUKEMIA; NON-HODGKINS-LYMPHOMA; HUMAN-B-CELLS; EPSTEIN-BARR-VIRUS; PEDIATRIC-ONCOLOGY-GROUP; INTERCELLULAR-ADHESION MOLECULE-1; ACUTE NONLYMPHOCYTIC LEUKEMIA; FLOW CYTOMETRIC ANALYSIS C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. RP Freedman, AS (reprint author), HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV HEMATOL MALIGNANCIES,BOSTON,MA 02115, USA. FU NCI NIH HHS [CA55207] NR 345 TC 21 Z9 21 U1 1 U2 3 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0735-7907 J9 CANCER INVEST JI Cancer Invest. PY 1996 VL 14 IS 3 BP 252 EP 276 DI 10.3109/07357909609012148 PG 25 WC Oncology SC Oncology GA UL401 UT WOS:A1996UL40100011 PM 8630688 ER PT J AU Ueki, K Ono, Y Hensen, JW Efird, JT vonDeimling, A Louis, DN AF Ueki, K Ono, Y Hensen, JW Efird, JT vonDeimling, A Louis, DN TI CDKN2/pl6 or RB alterations occur in the majority of glioblastomas and are inversely correlated SO CANCER RESEARCH LA English DT Article ID BRAIN-TUMOR SPECIMENS; ARCHIVAL AB p16 is involved in a cell cycle regulatory cascade that includes cyclin-dependent kinase 4 (cdk4), cyclin D1, and pRb (retinoblastoma). Alterations of each of these components have been described in primary human glioblastoma multiforme (GBM) or in GBM cell lines. Because perturbation of any component in this pathway may have similar oncogenic effects, we studied the relationship between abnormalities of CDKN2/p16 and RB, the two commonly involved tumor suppressor genes, in 55 astrocytic gliomas (42 GBMs, 8 anaplastic astrocytomas, and 5 astrocytomas). By using comparative multiplex PCR, homozygous deletions of the CDKN2/ p16 gene were detected in 24 GBMs (57%) and in 2 anaplastic astrocytomas. Two additional GBMs and one anaplastic astrocytoma had allelic loss of chromosome 9p, as assessed by microsatellite polymorphisms flanking the CDKN2/p16 region. Single-strand conformation polymorphism and DNA sequencing analysis of all three coding exons of CDKN2/p16 revealed a frameshift mutation (four-bp deletion) in one of the three GBMs that had lost the remaining 9p allele. Allelic loss of chromosome 13q at the RB gene, RB gene mutations, or loss of pRb expression was noted in 14 GBMs (33%) and 2 anaplastic astrocytomas. Thirty-six of 42 GBMs (86%) had alterations of either CDKN2/p16 (n = 22), RB (n = 10), or both (n = 4); these two genetic changes, however, were relatively exclusive (P = 0.003). Furthermore, of the six GBMs without either CDKN2/p16 or RB gene abnormalities, one case had CDK4 gene amplification. These data indicate that the vast majority of GBMs probably have inactivation of the p16-cdk4/cyclin D1-pRb pathway. The findings also provide corroborative evidence that CDKN2/p16 and RB are the critical glioma tumor suppressor genes on chromosomes 9p and 13q, respectively. C1 MASSACHUSETTS GEN HOSP,MOLEC NEUROONCOL LAB,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,NEUROSURG SERV,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,NEUROL SERV,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT RADIAT ONCOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. UNIV HOSP BONN,INST NEUROPATHOL,BONN,GERMANY. RI von Deimling, Andreas/F-7774-2013 OI von Deimling, Andreas/0000-0002-5863-540X FU NCI NIH HHS [CA 57683] NR 32 TC 364 Z9 367 U1 1 U2 3 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1996 VL 56 IS 1 BP 150 EP 153 PG 4 WC Oncology SC Oncology GA TM481 UT WOS:A1996TM48100027 PM 8548755 ER PT J AU Hermanson, M Funa, K Koopmann, J Maintz, D Waha, A Westermark, B Heldin, CH Wiestler, OD Louis, DN vonDeimling, A Nister, M AF Hermanson, M Funa, K Koopmann, J Maintz, D Waha, A Westermark, B Heldin, CH Wiestler, OD Louis, DN vonDeimling, A Nister, M TI Association of loss of heterozygosity on chromosome 17p with high platelet-derived growth factor alpha receptor expression in human malignant gliomas SO CANCER RESEARCH LA English DT Article ID HUMAN GLIOBLASTOMA-MULTIFORME; POLYMERASE CHAIN-REACTION; PDGF-RECEPTOR; GENE AMPLIFICATION; HUMAN ASTROCYTOMAS; P53 MUTATIONS; MESSENGER-RNA; GLIAL ORIGIN; CDNA CLONING; CELL-LINES AB The aim of this study nas to examine platelet-derived growth factor alpha receptor (PDGFR-alpha) expression in gliomas of various degrees of malignancy and to correlate the findings with genetic alterations present in the same tumor samples. We analyzed 83 tumors by in situ hybridization using a PDGFR-alpha cRNA probe. Increased PDGFR-alpha mRNA expression was observed in astrocytic tumors of all stages of malignancy, although the highest levels were found in glioblastoma multiforme. To evaluate the frequency of PDGFR-alpha gene amplification, differential PCR requiring less DNA than Southern analysis was used with fluorescence-labeled primers corresponding to the kinase insert region of the PDGFR-alpha. Only 7 of 43 glioblastomas and none of the other tumors tested showed amplification of the PDGFR-alpha gene, suggesting that a mechanism other than gene amplification is responsible for the overexpression of PDGFR-alpha in glial brain tumors. Comparison of the in situ hybridization data with genetic alterations in the same tumor material showed a significant correlation of loss of heterozygosity on chromosome 17p (Fisher's exact, P < 0.0002) with high expression levels of PDGFR-alpha. Because that was the case in both low- and high-grade astrocytomas, our data imply that PDGFR-alpha is actively involved in tumor cell proliferation in early and late stages of glioma development. The association of PDGFR-alpha expression with a distinct subset of glioblastomas characterized by loss of heterozygosity 17p further supports the differentiation of these tumors into molecular variants. C1 BIOMED CTR,LUDWIG INST CANC RES,S-75124 UPPSALA,SWEDEN. UNIV UPPSALA HOSP,DEPT PATHOL,S-75185 UPPSALA,SWEDEN. UNIV BONN,MED CTR,DEPT NEUROPATHOL,D-53105 BONN,GERMANY. MASSACHUSETTS GEN HOSP,NEUROSURG SERV,MOLEC NEUROONCOL LAB,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT NEUROPATHOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. RI von Deimling, Andreas/F-7774-2013 OI von Deimling, Andreas/0000-0002-5863-540X FU NCI NIH HHS [CA 57683] NR 43 TC 152 Z9 161 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA PUBLIC LEDGER BLDG, SUITE 816, 150 S. INDEPENDENCE MALL W., PHILADELPHIA, PA 19106 SN 0008-5472 J9 CANCER RES JI Cancer Res. PD JAN 1 PY 1996 VL 56 IS 1 BP 164 EP 171 PG 8 WC Oncology SC Oncology GA TM481 UT WOS:A1996TM48100030 PM 8548759 ER PT J AU Woodgett, JR Avruch, J Kyriakis, J AF Woodgett, JR Avruch, J Kyriakis, J TI The stress activated protein kinase pathway SO CANCER SURVEYS LA English DT Article ID SIGNAL-TRANSDUCTION PATHWAYS; AMINO-TERMINAL KINASES; C-JUN; TRANSCRIPTIONAL ACTIVATION; PHOSPHORYLATION; BINDING; CONTAINS; ELK-1; SPECIFICITY; INTEGRATION C1 MASSACHUSETTS GEN HOSP,DIABET LAB,CHARLESTOWN,MA 02129. HARVARD UNIV,SCH MED,CHARLESTOWN,MA 02129. RP Woodgett, JR (reprint author), ONTARIO CANC INST,610 UNIV AVE,TORONTO,ON M5G 2M9,CANADA. RI Woodgett, Jim/F-1087-2010 OI Woodgett, Jim/0000-0003-3731-5797 NR 40 TC 76 Z9 76 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0261-2429 J9 CANCER SURV JI Cancer Surv. PY 1996 VL 27 BP 127 EP 138 PG 12 WC Oncology SC Oncology GA VP934 UT WOS:A1996VP93400008 PM 8909798 ER PT J AU Rivitz, SM Waltman, AC Kelsey, PB AF Rivitz, SM Waltman, AC Kelsey, PB TI Embolization of an hepatic artery pseudoaneurysm following laparoscopic cholecystectomy SO CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY LA English DT Article DE hepatic artery; pseudoaneurysm; therapeutic blockade; surgery complication; gallbladder, surgery ID COMPLICATIONS AB Vascular injuries during laparoscopic cholecystectomy can occur in an analogous fashion to biliary injuries, with potential laceration, transection, and occlusion of blood vessels, A patient presented with symptomatic hemobilia 1 month following laparoscopic cholecystectomy and was found to have a right hepatic artery pseudoaneurysm which communicated with the common bile duct, This was successfully embolized with several embolic agents, resulting in rapid resolution of all signs and symptoms. The patient has been free of symptoms during a follow-up period of 1 year. A brief discussion of hepatic artery pseudoaneurysms is presented. RP Rivitz, SM (reprint author), MASSACHUSETTS GEN HOSP,DIV VASC RADIOL,GRB-290,POB 9657,BOSTON,MA 02114, USA. NR 13 TC 33 Z9 33 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0174-1551 J9 CARDIOVASC INTER RAD JI Cardiovasc. Interv. Radiol. PD JAN-FEB PY 1996 VL 19 IS 1 BP 43 EP 46 DI 10.1007/s002709900009 PG 4 WC Cardiac & Cardiovascular Systems; Radiology, Nuclear Medicine & Medical Imaging SC Cardiovascular System & Cardiology; Radiology, Nuclear Medicine & Medical Imaging GA TT516 UT WOS:A1996TT51600009 PM 8653746 ER PT J AU Ravichandran, KS Collins, TL Burakoff, SJ AF Ravichandran, KS Collins, TL Burakoff, SJ TI CD4 and signal transduction SO CD4 MOLECULE SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Review ID T-CELL RECEPTOR; TYROSINE-PROTEIN-KINASE; AMINO-TERMINAL DOMAIN; GRB2 ADAPTER PROTEIN; ANTIGEN RECEPTOR; CROSS-LINKING; LYMPHOCYTE-T; PHYSICAL ASSOCIATION; PHOSPHATIDYLINOSITOL 3-KINASE; PHOSPHOLIPASE C-GAMMA-1 C1 HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. RP Ravichandran, KS (reprint author), DANA FARBER CANC INST,DIV PEDIAT ONCOL,44 BINNEY ST,BOSTON,MA 02115, USA. NR 97 TC 24 Z9 24 U1 2 U2 4 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 205 BP 47 EP 62 PG 16 WC Immunology; Microbiology SC Immunology; Microbiology GA BJ47G UT WOS:A1996BJ47G00003 PM 8575197 ER PT J AU Kandikonda, S Oda, D Niederman, R Sorkin, BC AF Kandikonda, S Oda, D Niederman, R Sorkin, BC TI Cadherin-mediated adhesion is required for normal growth regulation of human gingival epithelial cells SO CELL ADHESION AND COMMUNICATION LA English DT Article DE E-cadherin; P-cadherin; cell adhesion molecules; contact inhibition; growth regulation; gingival epithelium ID BETA-CATENIN; P-CADHERIN; TYROSINE PHOSPHORYLATION; L-CAM; INTERCELLULAR-ADHESION; MONOCLONAL-ANTIBODY; CYTOPLASMIC DOMAIN; CARCINOMA-CELLS; ALPHA-CATENIN; N-CADHERIN AB The cadherins are a family of cell membrane proteins that mediate calcium-dependent cell-cell adhesion. E-cadherin is required for the formation, differentiation, polarization and stratification of epithelia; P-cadherin is also expressed on many epithelia. We report here the first study of cadherin expression in immortalized human gingival epithelial cells (IHGK) and examine the role of cadherins in growth regulation of these cells. We found that the IHGK cells are similar to normal gingival epithelial cells in their cadherin expression and density-dependent inhibition of growth. The IHGK cells proliferate more rapidly at low calcium concentration (0.15 mM) than at physiological concentrations of calcium (1.8 mM) and magnesium (0.65 mM; Ca/Mg medium) suggesting that calcium is required for density-dependent regulation of proliferation. To evaluate the possibility that cadherin function is required for contact inhibition in these cells, we grew them in Ca/Mg medium in the presence of adhesion-blocking anti-cadherin monoclonal antibodies. At anti-E-cadherin concentrations sufficient to disrupt cell-cell adhesion, the proliferation of the IHGK cells was similar to that observed in medium containing 0.2 mM EDTA, Anti-P-cadherin had a much weaker effect on cell proliferation than anti-E-cadherin, and cells grown in medium containing both antibodies grew at intermediate rates. The increased proliferation of the IHGK cells in either low calcium medium or Ca/Mg medium containing adhesion-blocking anti-cadherin antibodies suggests that cadherin-mediated adhesion is required for density-dependent regulation of growth of these cells. C1 FORSYTH DENT CTR,BOSTON,MA 02115. OI Sorkin, Barbara/0000-0001-8198-6921 FU NICHD NIH HHS [N01-HD-2-3144]; NIDCR NIH HHS [DE08415, DE11098] NR 64 TC 47 Z9 48 U1 1 U2 3 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1061-5385 J9 CELL ADHES COMMUN JI Cell Adhes. Commun. PY 1996 VL 4 IS 1 BP 13 EP & DI 10.3109/15419069609010760 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA VE784 UT WOS:A1996VE78400002 PM 8870970 ER PT J AU Weitzman, JB Hemler, ME Brodt, P AF Weitzman, JB Hemler, ME Brodt, P TI Reduction of tumorigenicity by alpha(3) integrin in a rhabdomyosarcoma cell line SO CELL ADHESION AND COMMUNICATION LA English DT Article DE integrin; adhesion; neoplastic transformation; rhabdomyosarcoma; VLA-3 ID HUMAN-MELANOMA CELLS; KERATINOCYTE INTERCELLULAR-ADHESION; TUMOR-SUPPRESSOR GENE; EXTRACELLULAR-MATRIX; BETA-1 INTEGRINS; CARCINOMA-CELLS; T-CELLS; CYTOPLASMIC DOMAINS; MALIGNANT-MELANOMA; RECEPTOR COMPLEXES AB The expression levels of integrin adhesion receptors have often been correlated with neoplastic transformation and invasiveness, To investigate more definitively the role of the integrin VLA-3 (alpha(3) beta(1)) in tumor cell behavior, we transfected alpha(3) subunit cDNA into human rhabdomyosarcoma (RD) cells. Transfectants expressing high levels of alpha(3) beta(1) on their cell surface displayed an altered morphology and decreased anchorage-dependent growth in vitro. Cells expressing alpha(3) also displayed marked reduction in anchorage-independent growth in soft agar and in their ability to form tumors when injected subcutaneously into athymic nude mice. Thus, VLA-3 can repress the transformed phenotype of rhabdomyosarcoma tumor cells. Similar changes in morphology and growth characteristics were observed in cells expressing a chimeric molecule X3C4 in which the alpha(3) cytoplasmic domain had been exchanged with that of the alpha(4) integrin subunit. Therefore, alpha(3) inhibitory effects in RD cells appear not to require specific signalling through the alpha(3) cytoplasmic domain. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV TUMOR VIROL,BOSTON,MA 02115. MCGILL UNIV,DIV SURG RES,MONTREAL,PQ H3A 1A4,CANADA. OI Weitzman, Jonathan/0000-0001-8445-0102 FU NIGMS NIH HHS [GM38903] NR 74 TC 15 Z9 15 U1 0 U2 0 PU HARWOOD ACAD PUBL GMBH PI READING PA C/O STBS LTD, PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 1061-5385 J9 CELL ADHES COMMUN JI Cell Adhes. Commun. PY 1996 VL 4 IS 1 BP 41 EP & DI 10.3109/15419069609010762 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA VE784 UT WOS:A1996VE78400004 PM 8870972 ER PT J AU Li, WW Fishman, MC Yuan, JY AF Li, WW Fishman, MC Yuan, JY TI Prevention of apoptosis in CNTF-dependent neurons by a mutant ICE and by viral protein CrmA but not by proto-oncogene product Bcl-2 SO CELL DEATH AND DIFFERENTIATION LA English DT Article DE CNTF; ICE; Bcl-2; CrmA; apoptosis; programmed cell death ID CILIARY NEUROTROPHIC FACTOR; PROGRAMMED CELL-DEATH; INTERLEUKIN-1-BETA CONVERTING ENZYME; GROWTH-FACTOR; C-ELEGANS; CHOLINERGIC DIFFERENTIATION; CAENORHABDITIS-ELEGANS; SYMPATHETIC NEURONS; MOLECULAR-CLONING; COWPOX VIRUS AB The interleukin-1 beta converting enzyme (ICE) gene family, (homologues of C. elegans cell death gene product Ced-3) plays an important role in controlling programmed cell death. Nerve growth factor (NGF) promotes survival of cultured embryonic chicken dorsal root ganglion neurons. Ciliary ganglion neurons depend exclusively on ciliary neurotrophic factor (CNTF) for survival. Complete depletion of NGF or CNTF from culture medium induces apoptosis in both types of neurons. We can prevent apoptosis, due either to NGF or CNTF withdrawal and in either type of neuron, by overexpression of a mutant inactive ICE and an ICE inhibitor, the product of cowpox virus gene crmA. Bcl-2 does not prevent apoptosis in CNTF-dependent ciliary neurons or DRG neurons as it does in NGF-dependent neurons. These results suggest that neuronal cell death is mediated through a common effector mechanism involving the Ice family of genes, whereas different suppression mechanisms are engaged depending upon the specific neurotrophic factors present. C1 MASSACHUSETTS GEN HOSP EAST,CARDIOVASC RES CTR,BOSTON,MA 02129. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA. NR 52 TC 8 Z9 8 U1 0 U2 0 PU EDWARD ARNOLD PUBL LTD PI LONDON PA 338 EUSTON ROAD, LONDON, ENGLAND NW1 3BH SN 1350-9047 J9 CELL DEATH DIFFER JI Cell Death Differ. PD JAN PY 1996 VL 3 IS 1 BP 105 EP 112 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA TV049 UT WOS:A1996TV04900016 PM 17180061 ER PT J AU Moskowitz, MA Dalkara, T AF Moskowitz, MA Dalkara, T TI Nitric oxide and cerebral ischemia SO CELLULAR AND MOLECULAR MECHANISMS OF ISCHEMIC BRAIN DAMAGE SE ADVANCES IN NEUROLOGY LA English DT Article ID INHIBITOR; MICE; NOS C1 HARVARD UNIV,SCH MED,BOSTON,MA 02129. RP Moskowitz, MA (reprint author), MASSACHUSETTS GEN HOSP,DEPT NEUROL & NEUROSURG SERV,BOSTON,MA 02129, USA. RI Moskowitz, Michael/D-9916-2011 NR 9 TC 9 Z9 9 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 SN 0091-3952 J9 ADV NEUROL JI Adv.Neurol. PY 1996 VL 71 BP 365 EP 369 PG 5 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA BG71K UT WOS:A1996BG71K00027 PM 8790812 ER PT S AU Finklestein, SP AF Finklestein, SP BE Siesjo, BK Wieloch, T TI The potential use of neurotrophic growth factors in the treatment of cerebral ischemia SO CELLULAR AND MOLECULAR MECHANISMS OF ISCHEMIC BRAIN DAMAGE SE ADVANCES IN NEUROLOGY LA English DT Article ID REDUCES INFARCT SIZE; FACTOR BFGF; BRAIN INJURY; TIME-COURSE; ADULT-RATS; NEURONS; RECEPTOR; DAMAGE; GERBIL; IMMUNOREACTIVITY C1 HARVARD UNIV, SCH MED, BOSTON, MA 02129 USA. RP Finklestein, SP (reprint author), MASSACHUSETTS GEN HOSP, DEPT NEUROL, CNS GROWTH FACTOR RES LAB, BOSTON, MA 02129 USA. FU NIA NIH HHS [AG08207]; NINDS NIH HHS [NS10828] NR 40 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA E WASHINGTON SQ, PHILADELPHIA, PA 19105 USA SN 0091-3952 BN 0-781-70277-1 J9 ADV NEUROL JI Adv.Neurol. PY 1996 VL 71 BP 413 EP 418 PG 6 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA BG71K UT WOS:A1996BG71K00031 PM 8790816 ER PT J AU Wilens, TE Biederman, J Spencer, TJ AF Wilens, TE Biederman, J Spencer, TJ TI Attention-deficit hyperactivity disorder and the psychoactive substance use disorders SO CHILD AND ADOLESCENT PSYCHIATRIC CLINICS OF NORTH AMERICA LA English DT Review ID SEEKING COCAINE ABUSERS; PSYCHIATRIC-DISORDERS; RISK-FACTORS; DRUG-USE; ALCOHOL-ABUSE; FOLLOW-UP; PREADOLESCENT CHILDREN; CHILDHOOD PERSONALITY; DIFFICULT TEMPERAMENT; CONDUCT DISORDER AB There has been increasing interest in the overlap between attention-deficit hyperactivity disorder (ADHD) and psychoactive substance use disorder (PSUD). In this article the relationship between PSUD and ADHD and associated concurrent disorders such as conduct disorder is addressed. A review of the literature suggests a robust association between ADHD and PSUD; however, the precise nature of this association remains unclear. Diagnostic and treatment strategies for adolescents and adults with ADHD plus PSUD are discussed. C1 MASSACHUSETTS GEN HOSP, RES PROGRAM, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA USA. RP Wilens, TE (reprint author), MASSACHUSETTS GEN HOSP, PEDIAT PSYCHOPHARMACOL CLIN, ACC 725, BOSTON, MA 02114 USA. NR 107 TC 26 Z9 26 U1 2 U2 4 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 1056-4993 J9 CHILD ADOL PSYCH CL JI Child Adolesc. Psychiatr. N. Am. PD JAN PY 1996 VL 5 IS 1 BP 73 EP + PG 20 WC Psychiatry SC Psychiatry GA UZ890 UT WOS:A1996UZ89000007 ER PT S AU Karczmar, AG AF Karczmar, AG BE Klein, J Loffelholz, K TI Loewi's discovery and the XXI century SO CHOLINERGIC MECHANISMS: FROM MOLECULAR BIOLOGY TO CLINICAL SIGNIFICANCE SE Progress in Brain Research LA English DT Review CT 9th International Cholinergic Symposium (ISCM) CY JUN 07-10, 1995 CL MAINZ, GERMANY SP Johannes Gutenberg Univ Mainz, State Rheinland Pfalz, Deut Forschungsgemeinsch, Deut Gesell Exptl & Klin Pharm & Toxikol, Int Soc Neurochem, Bayer Ag, Germany, Boehringer Ingelheim, Germany, MSD, Germany, Byk Gulden, Germany, Hoffmann La Roche, Germany, Labotec, Germany, Madaus, Germany, Marion Merrell, Germany, Merz & Co, Germany, Pharmacia, Germany, Pharmacia Biotech, Germany, Roland, Germany, Schwabe, Germany, SmithKline Beecham, Germany, Upjohn, US ID NICOTINIC ACETYLCHOLINE-RECEPTORS; CHOLINERGIC MECHANISMS; POSTSYNAPTIC ACTIONS; ION CHANNELS; RAT-BRAIN; SYSTEM; NEUROTRANSMITTERS; PHOSPHOLIPIDS; SUBTYPES; EXAMPLE C1 US DEPT VET AFFAIRS, VET AFFAIRS EDWARD HINES JR HOSP, RES SERV, HINES, IL 60141 USA. RP Karczmar, AG (reprint author), LOYOLA UNIV, MED CTR, DEPT PHARMACOL, MAYWOOD, IL 60153 USA. FU NCRR NIH HHS [RR05368]; NINDS NIH HHS [NS15858, NS6455] NR 208 TC 9 Z9 10 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0079-6123 BN 0-444-82166-X J9 PROG BRAIN RES JI Prog. Brain Res. PY 1996 VL 109 BP 1 EP 27 PG 27 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA BJ05X UT WOS:A1996BJ05X00001 PM 9009689 ER PT J AU Murray, DR Freeman, GL AF Murray, DR Freeman, GL TI Tumor necrosis factor-alpha induces a biphasic effect on myocardial contractility in conscious dogs SO CIRCULATION RESEARCH LA English DT Article DE cytokine; left ventricular function septic shock; norepinephrine; epinephrine ID SYSTOLIC PRESSURE-VOLUME; CLOSED-CHEST DOGS; HUMAN SEPTIC SHOCK; NITRIC-OXIDE; FACTOR CHALLENGES; INTERFERON-GAMMA; END-EJECTION; HEART; DYSFUNCTION; PERFORMANCE AB Tumor necrosis factor-alpha (TNF-alpha) likely plays a role in the pathophysiology of myocardial depression observed in septic shock. To evaluate the hemodynamic effects of TNF-alpha in vivo while eliminating the influence of altered sympathetic tone, eight conscious chronically instrumented dogs were studied after pretreatment with propranolol (2 mg/kg) and atropine (2 mg). Using three sets of piezoelectric crystals to measure left ventricular (LV) volume and LV manometers to measure pressure, we determined load-independent parameters of LV systolic performance before, during, and after infusion of recombinant human TNF-alpha (rhTNF-alpha, 40 mu g/kg for 1 hour). Plasma was analyzed for epinephrine and norepinephrine. Between 1 and 7 hours of exposure, rhTNF-alpha induced significant increases in circulating catecholamines. Norepinephrine rose from 268.6+/-47.2 to 426.2+/-87.0 pg/mL (P<.05) at 1 hour and peaked at 921.2+/-156.8 pg/mL (P<.001) at 4 hours after initiating rhTNF-alpha treatment. Similarly, epinephrine increased from 130.2+/-30.9 to 884.5+/-210.2 pg/mL (P<.05) at 1 hour and peaked at 3195.3+/-476 pg/mL (P<.001) at 4 hours. Before the surge of circulating catecholamines and despite complete beta- adrenergic blockade, rhTNF-alpha induced a 7% to 40% increase in LV contractile performance during the 60-minute infusion. After this initial positive inotropic effect, rhTNF-alpha treatment led to precipitous systolic dysfunction between 2 and 7 hours of exposure; this myocardial depressant effect persisted at 25 hours. LV systolic performance declined to 19% to 35% of baseline values, depending on the specific contractile parameter evaluated. We conclude that rhTNF-alpha affects LV systolic function in a time-dependent biphasic manner. Increases in circulating catecholamines after rhTNF-alpha infusion cannot account for the early improvement in LV systolic performance. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Murray, DR (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT MED,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. NR 45 TC 111 Z9 119 U1 1 U2 1 PU AMER HEART ASSOC PI DALLAS PA 7272 GREENVILLE AVENUE, DALLAS, TX 75231-4596 SN 0009-7330 J9 CIRC RES JI Circ.Res. PD JAN PY 1996 VL 78 IS 1 BP 154 EP 160 PG 7 WC Cardiac & Cardiovascular Systems; Hematology; Peripheral Vascular Disease SC Cardiovascular System & Cardiology; Hematology GA TM904 UT WOS:A1996TM90400019 PM 8603499 ER PT J AU Aleryani, S Kabakibi, A CluetteBrown, J Laposata, M AF Aleryani, S Kabakibi, A CluetteBrown, J Laposata, M TI Fatty acid ethyl ester synthase, an enzyme for nonoxidative ethanol metabolism, is present in serum after liver and pancreatic injury SO CLINICAL CHEMISTRY LA English DT Article DE fatty acids; lipids; chromatography, thin-layer; gas chromatography mass spectrometry; enzyme activity ID ADIPOSE-TISSUE AB Fatty acid ethyl esters (FAEE), esterification products of ethanol and fatty acids, have been implicated as mediators of ethanol-induced organ damage. Because cytosolic enzymes such as aspartate aminotransferase, lipase, and amylase appear in the blood after liver or pancreatic damage, we hqpothesized that FAEE synthase, a which is both cytosolic and membrane bound, is also released into the blood of patients with liver or pancreatic disease, We used a method involving thin-layer chromatography coupled with gas chromatography-mass spectrometry to reliably identify and quantify FAEE. In this study, we demonstrated that patients with liver or pancreatic disease release FAEE synthase into their plasma in amounts proportional to the amount of aspartate aminotransferase (r = 0.78), amylase (r = 0.65), and lipase (r = 0.63), These data indicate that liver and pancreatic damage results in release of FAEE synthase into the blood, The presence of FAEE synthase in plasma permits nonoxidative ethanol metabolism in the plasma. C1 MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. NR 18 TC 18 Z9 18 U1 0 U2 0 PU AMER ASSOC CLINICAL CHEMISTRY PI WASHINGTON PA 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 SN 0009-9147 J9 CLIN CHEM JI Clin. Chem. PD JAN PY 1996 VL 42 IS 1 BP 24 EP 27 PG 4 WC Medical Laboratory Technology SC Medical Laboratory Technology GA TR040 UT WOS:A1996TR04000007 PM 8565227 ER PT J AU Treon, SP Chabner, BA AF Treon, SP Chabner, BA TI Concepts in use of high-dose methotrexate therapy SO CLINICAL CHEMISTRY LA English DT Article; Proceedings Paper CT 19th Arnold O Beckman Conference in Clinical Chemistry - Drugs and Toxins in Clinical Laboratory Practice CY FEB 05-06, 1996 CL SAN DIEGO, CA DE leukemia; osteosarcoma; monitoring therapy; pharmacokinetics; urine; leucovorin ID BREAST CANCER-CELLS; ACUTE LYMPHOCYTIC-LEUKEMIA; CITROVORUM FACTOR RESCUE; DIHYDROFOLATE-REDUCTASE; OSTEO-SARCOMA; LEUCOVORIN RESCUE; RHESUS-MONKEYS; PREOPERATIVE CHEMOTHERAPY; ADJUVANT CHEMOTHERAPY; DNA-SYNTHESIS AB In cancer chemotherapy, routine monitoring of drug concentrations has been practical only for methotrexate (MTX). The primary setting for pharmacokinetic monitoring of MTX is its use in high doses (HDMTX) for adjuvant therapy of osteosarcoma, for single-agent treatment of intracranial lymphomas, and in combination therapy of childhood leukemia as well as adult and pediatric non-Hodgkin lymphomas. Typically, HDMTX is infused in doses of 3-15 g/m(2) over a period of 6-24 h. Precautions must be taken to ensure a high urine flow and an alkaline urine pH, so as to prevent precipitation of MTX in urine. Patients with decreased renal function, advanced in age, and taking nonsteroidal anti-inflammatory drugs or nephrotoxic agents are at increased risk of developing renal dysfunction during MTX infusion, thus being placed at high risk for toxicity. At the end of HDMTX infusion, and periodically thereafter for 24-48 h, drug concentrations are measured to assure that the disappearance rate of MTX from plasma is occurring at a normal rate. Also, at the end of HDMTX infusion, the patient is given leucovorin (5-formyl-tetrahydrofolic acid; LV), which replenishes intracellular stores of reduced folate and attenuates the toxicity secondary to HDMTX. In the presence of inappropriately high concentrations of MTX, routine doses of LV will be ineffective; the dose of LV required must be increased in proportion to the MTX concentration it faces in plasma. In practice, routine monitoring of plasma MTX concentrations allows early detection of abnormal clearance, as well as institution of early and effective countermeasures, including the use of increased and prolonged LV rescue. C1 HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,DIV HEMATOL & ONCOL,BOSTON,MA 02114. NR 66 TC 69 Z9 79 U1 1 U2 3 PU AMER ASSOC CLINICAL CHEMISTRY PI WASHINGTON PA 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 SN 0009-9147 J9 CLIN CHEM JI Clin. Chem. PY 1996 VL 42 IS 8 BP 1322 EP 1329 PN 2 PG 8 WC Medical Laboratory Technology SC Medical Laboratory Technology GA VA556 UT WOS:A1996VA55600005 PM 8697606 ER PT J AU Craig, WA AF Craig, WA TI The role of isepamicin in the treatment of severe hospital infections - Foreword SO CLINICAL DRUG INVESTIGATION LA English DT Editorial Material RP Craig, WA (reprint author), UNIV WISCONSIN HOSP & CLIN,WILLIAM S MIDDLETON MEM VET ADM HOSP,MADISON,WI 53792, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ADIS INTERNATIONAL LTD PI AUCKLAND PA 41 CENTORIAN DR, PRIVATE BAG 65901, MAIRANGI BAY, AUCKLAND 10, NEW ZEALAND SN 1173-2563 J9 CLIN DRUG INVEST JI Clin. Drug Invest. PY 1996 VL 12 SU 1 BP R9 EP R10 PG 2 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA VP689 UT WOS:A1996VP68900001 ER PT J AU Musher, DM Groover, JE Graviss, EA Baughn, RE AF Musher, DM Groover, JE Graviss, EA Baughn, RE TI The lack of association between aging and postvaccination levels of IgG antibody to capsular polysaccharides of Streptococcus pneumoniae SO CLINICAL INFECTIOUS DISEASES LA English DT Article ID CELL-WALL POLYSACCHARIDE; PNEUMOCOCCAL VACCINE; RESPONSES; REVACCINATION; IMMUNIZATION; PERSISTENCE; INFECTION; ADULTS; SERUM AB One possible explanation for the apparently reduced efficacy of pneumococcal vaccine in elderly subjects is that IgG responses to pneumococcal capsular polysaccharides (PPSs) decline with aging. We administered pneumococcal vaccine to 118 adults who ranged in age from 20 to 93 years; 33 were greater than or equal to 70 years old. Four to 6 weeks later, we measured IgG reactive with PPSs from 10 commonly infecting serotypes of Streptococcus pneumoniae. By regression analysis, a slight but nonsignificant increase in anti-PPS IgG was observed with increased age for six serotypes and a nonsignificant decrease was observed for four. Mean IgG levels and the percentage of subjects with IgG levels greater than or equal to 1 mu g/mL were no different among persons greater than or equal to 70 years of age than among those less than or equal to 69 years of age, These results show no consistent effect of aging on anti-PPS IgG levels 4-6 weeks after pneumococcal vaccination. C1 BAYLOR COLL MED,DEPT MED,HOUSTON,TX 77030. BAYLOR COLL MED,DEPT MICROBIOL IMMUNOL,HOUSTON,TX 77030. RP Musher, DM (reprint author), DEPT VET AFFAIRS MED CTR,INFECT DIS SECT,MED SERV,ROOM 4B-370,2002 HOLCOMBE BLVD,HOUSTON,TX 77030, USA. NR 18 TC 28 Z9 28 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 1058-4838 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD JAN PY 1996 VL 22 IS 1 BP 165 EP 167 PG 3 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA TP780 UT WOS:A1996TP78000032 PM 8824989 ER PT J AU ORielly, M Chew, FS AF ORielly, M Chew, FS TI The scintigraphic features of giant-cell tumors in relation to other imaging modalities SO CLINICAL NUCLEAR MEDICINE LA English DT Article ID BONE; PATTERNS; LESIONS; MR AB Thirty-three patients with untreated giant-cell tumors of bone were studied with Tc-99m phosphate scintigraphy. The scintigraphic patterns of the tumors were varied and the tumors exhibited extended uptake of radioactivity in the majority of cases. The homogeneously diffuse pattern and the nonhomogeneous ''doughnut'' and ''blob'' patterns are described. The scintigraphic patterns of giant-cell tumors are considered in the light of the radiographic, angiographic, CT, and MR imaging studies and the histopathologic findings. C1 MASSACHUSETTS GEN HOSP,DEPT BONE & JOINT RADIOL,BOSTON,MA. OI Chew, Felix/0000-0003-2711-2013 NR 17 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0363-9762 J9 CLIN NUCL MED JI Clin. Nucl. Med. PD JAN PY 1996 VL 21 IS 1 BP 43 EP 48 PG 6 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TQ377 UT WOS:A1996TQ37700011 ER PT J AU Hilsenroth, MJ Handler, L Blais, MA AF Hilsenroth, MJ Handler, L Blais, MA TI Assessment of narcissistic personality disorder: A multi-method review SO CLINICAL PSYCHOLOGY REVIEW LA English DT Review ID DSM-III-R; STRUCTURED CLINICAL INTERVIEW; SELF-REPORT QUESTIONNAIRES; TERM FOLLOW-UP; AXIS-II; MCMI-II; ANTISOCIAL PERSONALITY; OBJECT REPRESENTATION; CONCURRENT VALIDITY; PANIC DISORDER AB This review examines the available empirical data for the diagnosis of narcissistic personality disorder (NPD) for three methods of assessment: semi-structured interviews, self-report inventories, and projective techniques. Issues of reliability, validity, and clinical utility are examined for each instrument (or scale). An overview of the relative advantages, disadvantages, and empirical support for each method of assessment in the diagnosis of NPD is presented in a discussion after the review of the salient literature. In general, it was found that semi-structured interviews are a fairly reliable and valid method of diagnosis for Axis II disorders but, for the most part, these studies have used woefully small samples of NPDs. In general, self-report instruments were best at screening for the presence or absence of personality disorder; identifying members of personality disorder clusters, and identifying negative instances of specific personality disorders or clusters. Self-report inventories and the structured interviews are often in disagreement concerning presence of specific personality pathology. In general, previous studies have found the tendency for self-report measures to diagnose personality disorders at much higher frequencies than do clinicians. Moreover, self-report measures frequently attributed two or more personality disorders to a particular individual. Additionally, research with projective methods over the last decade has shown this mode of assessment to be useful in the differential diagnosis of NPD both related and unrelated personality disorders. It seems prudent that the clinician and researcher alike should employ multiple methods of measurement and utilize information in a systematic and theoretical fashion when evaluating a patient for NPD diagnosis. Copyright (C) 1996 Elsevier Science Ltd C1 UNIV TENNESSEE,KNOXVILLE,TN 37996. MASSACHUSETTS GEN HOSP,DEPT PSYCHIAT,BOSTON,MA 02114. HARVARD UNIV,SCH MED,CAMBRIDGE,MA 02138. RP Hilsenroth, MJ (reprint author), UNIV ARKANSAS,DEPT PSYCHOL,216 MEM HALL,FAYETTEVILLE,AR 72701, USA. NR 132 TC 28 Z9 28 U1 14 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0272-7358 J9 CLIN PSYCHOL REV JI Clin. Psychol. Rev. PY 1996 VL 16 IS 7 BP 655 EP 683 DI 10.1016/S0272-7358(96)00038-4 PG 29 WC Psychology, Clinical SC Psychology GA VU921 UT WOS:A1996VU92100004 ER PT J AU Krosnick, A AF Krosnick, A TI Untitled - Comment SO CLINICAL THERAPEUTICS LA English DT Editorial Material RP Krosnick, A (reprint author), JOSLIN DIABET CTR,BOSTON,MA 02215, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 SN 0149-2918 J9 CLIN THER JI Clin. Ther. PD JAN-FEB PY 1996 VL 18 IS 1 BP 55 EP 55 DI 10.1016/S0149-2918(96)80178-0 PG 1 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TZ545 UT WOS:A1996TZ54500005 ER PT J AU Calder, AJ Young, AW Rowland, D Perrett, DI Hodges, JR Etcoff, NL AF Calder, AJ Young, AW Rowland, D Perrett, DI Hodges, JR Etcoff, NL TI Facial emotion recognition after bilateral amygdala damage: Differentially severe impairment of fear SO COGNITIVE NEUROPSYCHOLOGY LA English DT Article ID FACE PROCESSING IMPAIRMENTS; BRAIN INJURY; EXPRESSIONS; PERCEPTION; IDENTITY; DISCRIMINATION; MONKEYS; CORTEX AB Although the amygdala is widely believed to have a role in the recognition of emotion, a central issue concerns whether it is involved in the recognition of all emotions or whether it is more important to some emotions than to others. We describe studies of two people, DR and SE, with impaired recognition of facial expressions in the context of bilateral amygdala damage. When tested with photographs showing facial expressions of emotion from the Ekman and Friesen (1976) series, both DR and SE showed deficits in the recognition of fear. Problems in recognising fear were also found using photographic quality images interpolated (''morphed'') between prototypes of the six emotions in the Ekman and Friesen (1976) series to create a hexagonal continuum (running from happiness to surprise to fear to sadness to disgust to anger to happiness). Control subjects identified these morphed images as belonging to distinct regions of the continuum, corresponding to the nearest prototype expression. However, DR and SE were impaired on this task, with problems again being most clearly apparent in the region of the fear prototype, An equivalent test of recognition of morphed identities of six famous faces was performed normally by DR, confirming the dissociability of impairments affecting the recognition of identity and expression from the face. Further two-way forced-choice tests showed that DR was unable to tell fear from anger, but could tell happiness from sadness without difficulty. The finding that the recognition of fear can be differentially severely affected by brain injury is consistent with reports of the effects of bilateral amygdala damage in another case (Adolphs, Tranel, Damasio, & Damasio, 1994, 1995). The recognition of facial expressions of basic emotions may therefore be linked, to some extent, to specific neural substrates. C1 UNIV ST ANDREWS,ST ANDREWS,FIFE,SCOTLAND. UNIV CAMBRIDGE,ADDENBROOKES HOSP,NEUROL UNIT,CAMBRIDGE CB2 1TN,ENGLAND. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CHARLESTOWN,MA. RP Calder, AJ (reprint author), MRC,APPL PSYCHOL UNIT,15 CHAUCER RD,CAMBRIDGE CB2 2EF,ENGLAND. RI Young, Andy/G-2189-2011 OI Young, Andy/0000-0002-1202-6297 NR 47 TC 411 Z9 418 U1 4 U2 42 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE, EAST SUSSEX, ENGLAND BN3 2FA SN 0264-3294 J9 COGNITIVE NEUROPSYCH JI Cogn. Neuropsychol. PY 1996 VL 13 IS 5 BP 699 EP 745 DI 10.1080/026432996381890 PG 47 WC Psychology; Psychology, Experimental SC Psychology GA VE793 UT WOS:A1996VE79300005 ER PT J AU Gillespie, PG Hasson, T Garcia, JA Corey, DP AF Gillespie, PG Hasson, T Garcia, JA Corey, DP TI Multiple myosin isozymes and hair-cell function SO COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY LA English DT Article; Proceedings Paper CT 61st Cold Spring Harbor Symposium on Function and Dysfunction in the Nervous System CY 1996 CL COLD SPRING HARBOR, NY SP Alza Corp, Amgen Inc, BASF Biores Corp, Becton Dickinson & Co, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chiron Corp, Chugal Res Inst Molec Med Inc, Diagnost Prod Corp, Dupont Merck Pharmaceut Co, Forest Labs Inc, Genentech Inc, Hoeshst Marion Roussel Inc, Hoffmann La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Eli Lilly & Co, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Appl Biosyst Div, Pfizer Inc, Pharmacia & Upjohn Inc, Res Genet Inc, Sandoz Res Inst, Schering Plough Corp, Sumitomo Pharmaceut Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC ID CALMODULIN-BINDING PROTEINS; MECHANOELECTRICAL TRANSDUCTION; UNCONVENTIONAL MYOSIN; ACTIN-FILAMENTS; BULLFROGS SACCULUS; ADAPTATION; STEREOCILIA; BUNDLE; COCHLEA; GENE C1 JOHNS HOPKINS UNIV, DEPT NEUROSCI, BALTIMORE, MD 21205 USA. YALE UNIV, DEPT BIOL, NEW HAVEN, CT 06520 USA. YALE UNIV, DEPT PATHOL, NEW HAVEN, CT 06520 USA. MASSACHUSETTS GEN HOSP, HOWARD HUGHES MED INST, DEPT NEUROBIOL, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, HOWARD HUGHES MED INST, DEPT NEUROL, BOSTON, MA 02114 USA. HARVARD UNIV, BOSTON, MA 02114 USA. RP Gillespie, PG (reprint author), JOHNS HOPKINS UNIV, DEPT PHYSIOL, BALTIMORE, MD 21205 USA. OI Corey, David/0000-0003-4497-6016; Barr-Gillespie, Peter/0000-0002-9787-5860 NR 79 TC 8 Z9 8 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI COLD SPRING HARBOR PA 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA SN 0091-7451 J9 COLD SPRING HARB SYM JI Cold Spring Harbor Symp. Quant. Biol. PY 1996 VL 61 BP 309 EP 318 PG 10 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA XD580 UT WOS:A1996XD58000033 PM 9246460 ER PT J AU Gusella, JF McNeil, S Persichetti, F Srinidhi, J Novelletto, A Bird, E Faber, P Vonsattel, JP Myers, RH MacDonald, ME AF Gusella, JF McNeil, S Persichetti, F Srinidhi, J Novelletto, A Bird, E Faber, P Vonsattel, JP Myers, RH MacDonald, ME TI Huntington's disease SO COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY LA English DT Article; Proceedings Paper CT 61st Cold Spring Harbor Symposium on Function and Dysfunction in the Nervous System CY 1996 CL COLD SPRING HARBOR, NY SP Alza Corp, Amgen Inc, BASF Biores Corp, Becton Dickinson & Co, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chiron Corp, Chugal Res Inst Molec Med Inc, Diagnost Prod Corp, Dupont Merck Pharmaceut Co, Forest Labs Inc, Genentech Inc, Hoeshst Marion Roussel Inc, Hoffmann La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Eli Lilly & Co, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Appl Biosyst Div, Pfizer Inc, Pharmacia & Upjohn Inc, Res Genet Inc, Sandoz Res Inst, Schering Plough Corp, Sumitomo Pharmaceut Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC ID MACHADO-JOSEPH DISEASE; DENTATORUBRAL-PALLIDOLUYSIAN ATROPHY; CAG REPEAT LENGTH; SPINOCEREBELLAR ATAXIA TYPE-1; LYMPHOBLASTOID CELL-LINES; AGE-OF-ONSET; TRINUCLEOTIDE-REPEAT; CLINICAL-FEATURES; DENTATORUBROPALLIDOLUYSIAN ATROPHY; PHENOTYPIC VARIATION C1 HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114. UNIV ROMA TOR VERGATA,DEPT BIOL,ROME,ITALY. MCLEAN HOSP,BRAIN TISSUE RESOURCE CTR,BELMONT,MA 02178. MASSACHUSETTS GEN HOSP,MOL NEUROPATHOL LAB,CHARLESTOWN,MA 02129. BOSTON UNIV,SCH MED,DEPT NEUROL,BOSTON,MA 02118. RP Gusella, JF (reprint author), MASSACHUSETTS GEN HOSP,MOL NEUROGENET UNIT,CHARLESTOWN,MA 02129, USA. OI Novelletto, Andrea/0000-0002-1146-7680 FU NINDS NIH HHS [NS-16367, NS-32765] NR 80 TC 28 Z9 28 U1 0 U2 4 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0091-7451 J9 COLD SPRING HARB SYM JI Cold Spring Harbor Symp. Quant. Biol. PY 1996 VL 61 BP 615 EP 626 PG 12 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA XD580 UT WOS:A1996XD58000061 PM 9246488 ER PT J AU MacDonald, ME Duyao, M Calzonetti, T Auerbach, A Ryan, A Barnes, G White, JK Auerbach, W Vonsattel, JP Gusella, JF Joyner, AL AF MacDonald, ME Duyao, M Calzonetti, T Auerbach, A Ryan, A Barnes, G White, JK Auerbach, W Vonsattel, JP Gusella, JF Joyner, AL TI Targeted inactivation of the mouse Huntington's disease gene homolog Hdh SO COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY LA English DT Article; Proceedings Paper CT 61st Cold Spring Harbor Symposium on Function and Dysfunction in the Nervous System CY 1996 CL COLD SPRING HARBOR, NY SP Alza Corp, Amgen Inc, BASF Biores Corp, Becton Dickinson & Co, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chiron Corp, Chugal Res Inst Molec Med Inc, Diagnost Prod Corp, Dupont Merck Pharmaceut Co, Forest Labs Inc, Genentech Inc, Hoeshst Marion Roussel Inc, Hoffmann La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Eli Lilly & Co, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Appl Biosyst Div, Pfizer Inc, Pharmacia & Upjohn Inc, Res Genet Inc, Sandoz Res Inst, Schering Plough Corp, Sumitomo Pharmaceut Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC ID CAG REPEAT; NEURODEGENERATIVE DISEASES; EMBRYONIC LETHALITY; EXPRESSION; BRAIN; PROTEIN; REGION; CONSERVATION; CHROMOSOME-5; LOCALIZATION C1 NYU,MED CTR,SKIRBALL INST BIOMOL MED,DEV GENET PROGRAM,NEW YORK,NY 10016. MASSACHUSETTS GEN HOSP,LAB MOL NEUROPATHOL,CHARLESTOWN,MA 02129. HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114. RP MacDonald, ME (reprint author), MASSACHUSETTS GEN HOSP,MOL NEUROGENET UNIT,CHARLESTOWN,MA 02129, USA. FU NINDS NIH HHS [NS-32765, NS-16367] NR 47 TC 4 Z9 4 U1 2 U2 2 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0091-7451 J9 COLD SPRING HARB SYM JI Cold Spring Harbor Symp. Quant. Biol. PY 1996 VL 61 BP 627 EP 638 PG 12 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA XD580 UT WOS:A1996XD58000062 PM 9246489 ER PT J AU Williamson, TL Marszalek, JR Vechio, JD Bruijn, LI Lee, MK Xu, Z Brown, RH Cleveland, DW AF Williamson, TL Marszalek, JR Vechio, JD Bruijn, LI Lee, MK Xu, Z Brown, RH Cleveland, DW TI Neurofilaments, radial growth of axons, and mechanisms of motor neuron disease SO COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY LA English DT Article; Proceedings Paper CT 61st Cold Spring Harbor Symposium on Function and Dysfunction in the Nervous System CY 1996 CL COLD SPRING HARBOR, NY SP Alza Corp, Amgen Inc, BASF Biores Corp, Becton Dickinson & Co, Boehringer Mannheim Corp, Bristol Myers Squibb Co, Chiron Corp, Chugal Res Inst Molec Med Inc, Diagnost Prod Corp, Dupont Merck Pharmaceut Co, Forest Labs Inc, Genentech Inc, Hoeshst Marion Roussel Inc, Hoffmann La Roche Inc, Johnson & Johnson, Kyowa Hakko Kogyo Co Ltd, Life Technol Inc, Eli Lilly & Co, Oncogene Sci Inc, Pall Corp, Perkin Elmer Corp, Appl Biosyst Div, Pfizer Inc, Pharmacia & Upjohn Inc, Res Genet Inc, Sandoz Res Inst, Schering Plough Corp, Sumitomo Pharmaceut Co Ltd, Wyeth Ayerst Res, Zeneca Grp PLC ID AMYOTROPHIC-LATERAL-SCLEROSIS; GANGLION-CELL NEURONS; TRANSGENIC MICE; POSTTRANSLATIONAL MODIFICATION; EPIDERMOLYTIC HYPERKERATOSIS; NONNEURONAL CELLS; SCHWANN-CELLS; SCIATIC-NERVE; MOUSE MODEL; IN-VIVO C1 UNIV CALIF SAN DIEGO,DIV CELL & MOL MED,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093. UNIV CALIF SAN DIEGO,DEPT NEUROSCI,LA JOLLA,CA 92093. JOHNS HOPKINS UNIV,SCH MED,NEUROPATHOL LAB,BALTIMORE,MD 21205. WORCESTER FDN BIOMED RES,SHREWSBURY,MA 01545. MASSACHUSETTS GEN HOSP,DAY LAB NEUROMUSCULAR RES,BOSTON,MA 02114. RP Williamson, TL (reprint author), UNIV CALIF SAN DIEGO,LUDWIG INST CANC RES,LA JOLLA,CA 92093, USA. RI Lee, Michael/D-9491-2013 OI Lee, Michael/0000-0001-5865-9682 FU NINDS NIH HHS [NS-27036] NR 71 TC 24 Z9 25 U1 0 U2 2 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 0091-7451 J9 COLD SPRING HARB SYM JI Cold Spring Harbor Symp. Quant. Biol. PY 1996 VL 61 BP 709 EP 723 PG 15 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA XD580 UT WOS:A1996XD58000070 PM 9246497 ER PT B AU Bergwitz, C Klein, P Juppner, H AF Bergwitz, C Klein, P Juppner, H BE Dacke, C Danks, J Caple, I Flik, G TI Molecular cloning of complementary DNAs encoding the Xenopus laevis (Daudin) PTH/PTHrP receptor SO COMPARATIVE ENDOCRINOLOGY OF CALCIUM REGULATION LA English DT Proceedings Paper CT Satellite Meeting on the Comparative Endocrinology of Calcium Metabolism, at the XIIth International Conference on Calcium Regulating Hormones (ICCRH) CY FEB 14-19, 1995 CL ROYAL MELBOURNE ZOO, MELBOURNE, AUSTRALIA SP Univ Melbourne Vet Sch, Beckman Instrument Co HO ROYAL MELBOURNE ZOO RP Bergwitz, C (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,ENDOCRINE UNIT,DEPT MED,BOSTON,MA 02114, USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU SOCIETY ENDOCRINOLOGY PI BRISTOL PA 17/18 THE COURTYARD, WOODLANDS ALMONDSBURY, BRISTOL, ENGLAND BS12 4NQ BN 1-898099-08-1 PY 1996 BP 97 EP 102 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA BH19B UT WOS:A1996BH19B00009 ER PT J AU Lafer, B Nierenberg, AA Rosenbaum, JF Fava, M AF Lafer, B Nierenberg, AA Rosenbaum, JF Fava, M TI Outpatients with DSM-III-R versus DSM-IV melancholic depression SO COMPREHENSIVE PSYCHIATRY LA English DT Article AB The purpose of this study was to compare outpatients who met DSM-III-R versus DSM-IV criteria for melancholia. Of 176 consecutive outpatients with unipolar depression, 40 (22.7%) met DSM-III-R criteria and 29 (16.5%) met DSM-IV criteria for melancholia. Patients with DSM-IV melancholia had higher mean scores on measurements of clinical severity as compared with those who qualified for a DSM-III-R diagnosis. These results suggest that the criteria for melancholia proposed in the DSM-IV are more restrictive and define a more severely depressed population than criteria in the DSM-III-R. Copyright (C) 1996 by W.B. Saunders Company C1 MASSACHUSETTS GEN HOSP,CLIN NEUROPHYSIOL LAB,DEPRESS RES PROGRAM,BOSTON,MA 02114. RI Lafer, Beny/C-1055-2012; Lafer, Beny/F-9390-2015 OI Lafer, Beny/0000-0002-6132-9999; Lafer, Beny/0000-0002-6132-9999 NR 9 TC 13 Z9 13 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0010-440X J9 COMPR PSYCHIAT JI Compr. Psychiat. PD JAN-FEB PY 1996 VL 37 IS 1 BP 37 EP 39 DI 10.1016/S0010-440X(96)90048-6 PG 3 WC Psychiatry SC Psychiatry GA TP515 UT WOS:A1996TP51500006 PM 8770524 ER PT J AU Pedrozo, HA Schwartz, Z Dean, DD Wiederhold, ML Boyan, BD AF Pedrozo, HA Schwartz, Z Dean, DD Wiederhold, ML Boyan, BD TI Regulation of statoconia mineralization in Aplysia californica in vitro SO CONNECTIVE TISSUE RESEARCH LA English DT Article; Proceedings Paper CT 5th International Conference on the Chemistry and Biology of Mineralized Tissues CY OCT 22-27, 1995 CL KOHLER, WI DE calcium carbonate; Aplysia californica; statoconia; urease; carbonic anhydrase ID CARBONIC-ANHYDRASE; PARATHYROID-HORMONE; LOCALIZATION; OSTEOCLASTS; CALCITONIN; ORGANS AB Statoconia are calcium carbonate inclusions in the lumen of the gravity-sensing organ, the statocyst, of Aplysia californica. The aim of the present study was to examine the role of carbonic anhydrase and urease in statoconia mineralization in vitro, The experiments were performed using a previously described culture system (Pedrozo et al., J. Comp. Physiol. (A) 177:415-425). Inhibition of carbonic anhydrase by acetazolamide decreased statoconia production and volume, while inhibition of urease by acetohydroxamic acid reduced total statoconia number, but had no affect on statoconia volume, Inhibition of carbonic anhydrase initially increased and then decreased the statocyst pH, whereas inhibition of urease decreased statocyst pH at all times examined; simultaneous addition of both inhibitors also decreased pH. These effects were dose and time dependent. The results show that carbonic anhydrase and urease are required for statoconia formation and homeostasis, and for regulation of statocyst pH. This suggests that these two enzymes regulate mineralization at least partially through regulation of statocyst pH. C1 UNIV TEXAS, HLTH SCI CTR, DEPT ORTHOPAED, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT PHYSIOL, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT OTOLARYNGOL HEAD & NECK SURG, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT PERIODONT, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT BIOCHEM, SAN ANTONIO, TX 78284 USA. AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX 78284 USA. HEBREW UNIV JERUSALEM, HADASSAH FAC DENT MED, DEPT PERIODONT, IL-91905 JERUSALEM, ISRAEL. OI Dean, David/0000-0002-4512-9065 NR 25 TC 7 Z9 7 U1 1 U2 3 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 0300-8207 J9 CONNECT TISSUE RES JI Connect. Tissue Res. PY 1996 VL 35 IS 1-4 BP 317 EP 323 DI 10.3109/03008209609029206 PG 7 WC Cell Biology; Orthopedics SC Cell Biology; Orthopedics GA WL094 UT WOS:A1996WL09400046 PM 9084670 ER PT J AU Berra, A Dutt, JE Foster, CS AF Berra, A Dutt, JE Foster, CS TI Detection of herpes simplex virus type 1 by an in situ polymerase chain reaction technique SO CORNEA LA English DT Article DE herpes simplex virus type 1; in situ polymerase chain reaction; polymerase chain reaction; in situ hybridization ID RETINAL NECROSIS SYNDROME; DNA; CELLS; PCR; AMPLIFICATION; CONTAMINATION; INSITU; GENES AB The purpose of our study was to develop a method for detecting herpes simplex virus (HSV) DNA that combined the high sensitivity of the polymerase chain reaction (PCR) with the precise anatomical localization provided by in situ hybridization (ISH). We used insitu PCR (ISPCR), ISH, and standard PCR methods to determine the proportion of Vero cells carrying HSV-1-specific DNA before and after 1, 2, and 4 h of infection with HSV-1 or with HSV-2. Uninfected Vero cells and Vero cells infected with HSV-2 were never found to be positive for HSV-1 DNA by either ISPCR, ISH, or PCR. In contrast, using ISPCR, HSV-1 infected Vero cells showed an increase in the percentage of cells containing HSV-1 DNA from 20% at 1 h to 76% at 4 h after infection. Comparing the ISPCR results with ISH and standard PCR demonstrated that ISPCR was markedly more sensitive than ISH; in fact, the sensitivity of in situ PCR was similar to that seen with standard PCR. These results demonstrate that ISPCR is a highly sensitive method for amplifying genomic DNA sequences within intact single cells. This technique combines the exquisite sensitivity of conventional PCR technology with the precise cellular localization afforded by ISH. In addition, it allows for an accurate quantitative determination of the number of virally infected cells. C1 HARVARD UNIV,MASSACHUSETTS EYE & EAR INFIRM,SCH MED,HILLES IMMUNOL LAB,BOSTON,MA 02114. FU NEI NIH HHS [EY06008] NR 17 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0277-3740 J9 CORNEA JI Cornea PD JAN PY 1996 VL 15 IS 1 BP 55 EP 61 PG 7 WC Ophthalmology SC Ophthalmology GA TN069 UT WOS:A1996TN06900010 PM 8907381 ER PT J AU Wang, C Martyn, JAJ AF Wang, C Martyn, JAJ TI Burn injury alters beta-adrenergic receptor and second messenger function in rat ventricular muscle SO CRITICAL CARE MEDICINE LA English DT Article DE adrenergic receptor agonists; isoproterenol; burn injury; receptors, adrenergic; adrenoceptor agonists; catecholamines; enzymes; adenylate cyclase; sympathetic nervous system; second messenger systems; signal transduction ID G-PROTEINS; THERMAL-INJURY; HEART-FAILURE; ADENYLYL-CYCLASE; CARDIAC-OUTPUT; EPINEPHRINE; DOPAMINE; INFUSION; CATECHOLAMINES; ADRENOCEPTORS AB Objectives: The molecular pharmacologic bases for the attenuated cardiovascular and metabolic responses to catecholamines, after burn injury, have not been elucidated. In the present study, myocardial tissues were used as a model of beta-adrenergic receptors to study burn injury-induced alterations in receptors and in signal transduction. Design: Prospective study, randomized to treatment and control groups. Setting: University-hospital research laboratory. Subjects: Male Sprague Dawley rats (180 to 210 g). Interventions: A 50% body surface area burn or sham-burn was administered to the rats. Measurements and Main Results: Myocardial membranes were isolated at 24 hrs, 7 days and 14 days after 50% body surface area scald or sham injury. (-)I-125-iodocyanopindolol was used to assess maximal binding capacity and affinity of the beta-adrenergic receptor. Basal and stimulated concentrations of second messenger, cyclic adenosine monophosphate (cAMP), were also assessed. Production of cAMP during isoproterenol stimulation tested the integrity of the beta-adrenergic receptor-mediated signal transduction. Forskolin, which stimulates adenylate cyclase enzyme directly (bypassing the receptor and G protein) to produce cAMP, tested the efficacy of the enzyme itself. Maximal binding capacity was unaltered between the experimental and control groups, but the affinity (mean +/- SEM) was significantly decreased in burned animals at 7 days (125.4 +/- 15.5 picomoles [pmol]; p = .01) and at 14 days (216.7 +/- 50.7 pmol; p = .001) compared with controls (75.5 +/- 8.4 pmol). In different set experimental and control groups, basal concentrations of cAMP in myocardial membranes were significantly decreased in burned animals at 7 days (control 38.6 +/- 4.2 vs. 5.8 +/- 0.9 pmol/mg of protein/min; p = .003) and at 14 days (control 47.4 +/- 3.2 vs. 28.3 +/- 6.6 pmol/mg of protein/min; p = .002). The forskolin (direct)-stimulated synthesis of cAMP was decreased in burned animals at 24 hrs (control 339.0 +/- 40.5 vs. 214.4 +/- 16.6 pmol/mg of protein/min; p = .01), at 7 days (control 289.0 +/- 34.4 vs. 32 +/- 13.0 pmol/mg of protein/min; p = .01), and at 14 days (control 322.9 +/- 28.6 vs. 137.0 +/- 46.1 pmol/mg of protein/min; p = .01). The isoproterenol or receptor-mediated stimulation of cAMP production was also significantly (p < .001) impaired in burned animals compared with controls at 24 hrs (control 134.7 +/- 11.9 vs. 83.1 +/- 13.3 pmol/mg of protein/min), and at 14 days (control 128.2 +/- 7.2 vs. 92.8 +/- 17.7 pmol/mg of protein/min). Conclusion: The etiology of the decreased responses in the myocardium, to exogenous and endogenous beta-adrenergic receptor agonists after burn injury may be attributed to decreased affinity for ligands, and also to impaired receptor-mediated signal transduction and to decreased adenylate cyclase enzyme activity, resulting in decreased basal and stimulated second messenger (cAMP) production. C1 MASSACHUSETTS GEN HOSP,DEPT ANESTHESIA,CLIN & BIOCHEM PHARMACOL LAB,ANESTHESIA SERV,BOSTON,MA 02114. SHRINERS BURNS INST,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT ANESTHESIOL,BOSTON,MA. FU NIGMS NIH HHS [R0-1 GM31569-13] NR 46 TC 10 Z9 12 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0090-3493 J9 CRIT CARE MED JI Crit. Care Med. PD JAN PY 1996 VL 24 IS 1 BP 118 EP 124 DI 10.1097/00003246-199601000-00020 PG 7 WC Critical Care Medicine SC General & Internal Medicine GA TT354 UT WOS:A1996TT35400020 PM 8565516 ER PT J AU Weaver, DT AF Weaver, DT TI Regulation and repair of double-strand DNA breaks SO CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION LA English DT Review DE DNA double-strand break repair; Ku; DNA-dependent protein kinase; ionizing radiation; V(D)J recombination ID DEPENDENT PROTEIN-KINASE; RAY-SENSITIVE MUTANTS; RNA-POLYMERASE-I; WILD-TYPE P53; COMBINED IMMUNE-DEFICIENCY; CELL NUCLEAR ANTIGEN; HUMAN KU-AUTOANTIGEN; HAMSTER OVARY CELL; COMBINED IMMUNODEFICIENCY MUTATION; TERMINAL TRANSACTIVATION DOMAIN AB Double-strand break (DSB) repair in mammalian cells involves the DNA-dependent protein kinase (DNA-PK). DNA-PK is composed of three subunits, a catalytic kinase subunit CDNA-PKcs) mutated in the mouse scid complementation group and the Ku heterodimer. Ku-deficient and DNA-PKcs-deficient cell lines and an animal model have illuminated important features of the DSB repair pathway. Additional factors relevant to several essential functions of DNA replication may point to some overlap between DNA synthesis and DNA repair in eukaryotes. The DNA-PK complex may have broader functions based on its possible involvement in transcriptional regulation and cell cycle checkpoints. C1 DANA FARBER CANC INST,DIV TUMOR IMMUNOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOL GENET,BOSTON,MA 02115. NR 231 TC 46 Z9 46 U1 0 U2 0 PU BEGELL HOUSE INC PI NEW YORK PA 79 MADISON AVE, SUITE 1205, NEW YORK, NY 10016-7892 SN 1045-4403 J9 CRIT REV EUKAR GENE JI Crit. Rev. Eukaryot. Gene Expr. PY 1996 VL 6 IS 4 BP 345 EP 375 PG 31 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA VX058 UT WOS:A1996VX05800002 PM 8959372 ER PT J AU Bush, RK Hefle, SL AF Bush, RK Hefle, SL TI Food allergens SO CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION LA English DT Review ID AMINO-ACID-SEQUENCE; PISUM-SATIVUM-L; CROSS-REACTIVE ALLERGEN; IGE-BINDING PROTEINS; BIRD-EGG SYNDROME; ARA-H-I; SHRIMP-SENSITIVE INDIVIDUALS; SUBUNITS SYNTHESIZED INVITRO; SOYBEAN TRYPSIN-INHIBITORS; MAJOR COMPONENT PROTEINS C1 UNIV WISCONSIN,DEPT MED,MADISON,WI. UNIV WISCONSIN,INST FOOD RES,MADISON,WI 53706. UNIV NEBRASKA,FOOD ALLERGY RES & RESOURCE PROGRAM,LINCOLN,NE. RP Bush, RK (reprint author), WILLIAM S MIDDLETON MEM VET ADM MED CTR,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 349 TC 67 Z9 69 U1 1 U2 4 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 SN 1040-8398 J9 CRIT REV FOOD SCI JI Crit. Rev. Food Sci. Nutr. PY 1996 VL 36 SU S BP S119 EP S163 PG 45 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA VX188 UT WOS:A1996VX18800009 PM 8959381 ER PT J AU Papisov, M Weissleder, R AF Papisov, M Weissleder, R TI Drug delivery to lymphatic tissue SO CRITICAL REVIEWS IN THERAPEUTIC DRUG CARRIER SYSTEMS LA English DT Review DE lymph; lymph node; drug delivery; drug carrier; targeting; macromolecule; colloid; lymphography; cancer; AIDS; metastasis ID LPS-BINDING-PROTEIN; MAGNETIC-RESONANCE; MONOCLONAL-ANTIBODIES; MR LYMPHOGRAPHY; ILIOPELVIC LYMPHOSCINTIGRAPHY; MYOCARDIAL CAPILLARIES; LIPOPOLYSACCHARIDE LPS; FIBRONECTIN STRUCTURE; COMPLEMENT PROTEINS; RELAXATION-TIMES AB Efficient diagnosis and therapy of diseases affecting lymph nodes rely on the availability of drugs that are retained by lymph nodes. Intralymphatically or interstitially administered macromolecular carriers accumulate efficiently in draining lymph nodes. However, because of the high variability of lymphatic networks and drainage routes, systemic administration of lymphotropic carriers would be preferable and currently represents a major focus in lymphotropic drug design. This review focuses on advances in the development of intravenous drug carriers and briefly discusses agents used for local delivery. C1 HARVARD UNIV,SCH MED,CHARLESTOWN,MA 02129. RP Papisov, M (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIOL,NMR CTR,MR PHARMACEUT PROGRAM,CNY ROOM 5416,BLDG 149,13TH ST,CHARLESTOWN,MA 02129, USA. NR 151 TC 12 Z9 13 U1 1 U2 2 PU BEGELL HOUSE INC PI NEW YORK PA 79 MADISON AVE, SUITE 1205, NEW YORK, NY 10016-7892 SN 0743-4863 J9 CRIT REV THER DRUG JI Crit. Rev. Ther. Drug Carr. Syst. PY 1996 VL 13 IS 1-2 BP 57 EP 84 PG 28 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA VB495 UT WOS:A1996VB49500002 PM 8853959 ER PT J AU Mayer, EA Mulholland, MW AF Mayer, EA Mulholland, MW TI Large intestine SO CURRENT OPINION IN GASTROENTEROLOGY LA English DT Editorial Material RP Mayer, EA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CURE NEUROENTER BIOL GRP,BLDG 115,ROOM 223,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0267-1379 J9 CURR OPIN GASTROEN JI Curr. Opin. Gastroenterol. PD JAN PY 1996 VL 12 IS 1 BP 1 EP 2 PG 2 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TZ239 UT WOS:A1996TZ23900001 ER PT J AU Mayer, EA AF Mayer, EA TI Breaking down the functional and organic paradigm SO CURRENT OPINION IN GASTROENTEROLOGY LA English DT Article ID CORTICOTROPIN-RELEASING HORMONE; PITUITARY-ADRENAL AXIS; FIBROMYALGIA; ACTIVATION; DISEASE AB The most prevalent symptoms arising from disordered colonic function are altered bowel habits, commonly associated with abdominal discomfort. These symptoms can arise from a variety of different disorders that are traditionally classified into those of organic and those of functional etiology. In principle, disorders are referred to as functional when no specific structural, physiologic, or biochemical marker can be identified, although in practice, the search for markers is limited to endoscopic, radiologic, or histologic evidence. Generally, it is assumed that patients with functional colonic disorders suffer primarily from psychologic distress or psychiatric ''comorbidity.'' Due to a lack of specific biologic markers, symptom criteria have been developed that are thought to define distinct syndromes. I propose that in view of recent breakthroughs in our understanding of how the neuroendocrine control systems respond to perturbations of the external and internal environment of the organism in the form of the generalized stress response, the traditional separation into organic and functional is no longer appropriate. Instead, a concept is proposed in which disorders of the colon range from distinct patterns of alterations in the bidirectional dialogue between the central nervous system and the colon. RP Mayer, EA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CURE NEUROENTER BIOL GRP,BLDG 115,ROOM 223,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 32 TC 11 Z9 11 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0267-1379 J9 CURR OPIN GASTROEN JI Curr. Opin. Gastroenterol. PD JAN PY 1996 VL 12 IS 1 BP 3 EP 7 DI 10.1097/00001574-199612010-00002 PG 5 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TZ239 UT WOS:A1996TZ23900002 ER PT J AU Martinez, V Tache, Y AF Martinez, V Tache, Y TI Autonomic regulation of colonic epithelial and motor function SO CURRENT OPINION IN GASTROENTEROLOGY LA English DT Article AB Physiologic regulation of colonic function depends on the interaction among three systems: enteric, autonomic (sympathetic and parasympathetic), and immune. Visceral sensory information is transmitted via primary afferents and integrated at central (spinal and supraspinal) sites. Changes in the activity of these primary afferents are an important component in pathophysiologic alterations of colonic motor and secretory functions and seem to be a common mechanism underlying functional disorders of the gastrointestinal tract. Increased colorectal sensitivity to distention in irritable bowel syndrome may involve alterations of thoracolumbar afferents and the immune system in the colonic wall. Alterations in the properties of the enteric nervous system, epithelial transport, and somatic and visceral responses to visceral pain have been characterized during immune challenges and experimental colitis. RP Martinez, V (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,CURE,DIGEST DIS RES CTR,BLDG 115,ROOM 203,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 44 TC 0 Z9 0 U1 0 U2 0 PU RAPID SCIENCE PUBLISHERS PI LONDON PA 2-6 BOUNDARY ROW, LONDON, ENGLAND SE1 8NH SN 0267-1379 J9 CURR OPIN GASTROEN JI Curr. Opin. Gastroenterol. PD JAN PY 1996 VL 12 IS 1 BP 44 EP 49 PG 6 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TZ239 UT WOS:A1996TZ23900009 ER PT J AU Smith, BL AF Smith, BL TI The breast SO CURRENT PROBLEMS IN OBSTETRICS GYNECOLOGY AND FERTILITY LA English DT Review ID FINE-NEEDLE ASPIRATION; ESTROGEN-REPLACEMENT THERAPY; COMPARING TOTAL MASTECTOMY; LOBULAR CARCINOMA INSITU; INSTITUT-GUSTAVE-ROUSSY; MAMMOGRAPHIC FOLLOW-UP; CLINICAL-TRIAL; DIETARY-FAT; CANCER DETECTION; RADICAL-MASTECTOMY RP Smith, BL (reprint author), MASSACHUSETTS GEN HOSP,COMPREHENS BREAST HLTH CTR,DEPT SURG,BOSTON,MA 02114, USA. NR 190 TC 0 Z9 0 U1 1 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 8756-0410 J9 CURR PROB OBST GYN F JI Curr. Probl. Obstet. Gynecol. Fertil. PD JAN-FEB PY 1996 VL 19 IS 1 BP 5 EP 35 PG 31 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA TW655 UT WOS:A1996TW65500001 ER PT J AU Miura, M Yuan, JY AF Miura, M Yuan, JY TI Mechanisms of programmed cell death in Caenorhabditis elegans and vertebrates SO CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY, VOL 32 SE CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY LA English DT Review ID TUMOR-NECROSIS-FACTOR; INTERLEUKIN-1-BETA CONVERTING ENZYME; AVIAN RETICULOENDOTHELIOSIS VIRUS; CHEMOTHERAPY-INDUCED APOPTOSIS; HUMAN FOLLICULAR LYMPHOMA; NERVE GROWTH-FACTOR; C-MYC PROTEIN; BCL-2 GENE; LYMPHOCYTES-T; KAPPA-B C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. RP Miura, M (reprint author), MASSACHUSETTS GEN HOSP EAST,CARDIOVASC RES CTR,BOSTON,MA 02129, USA. NR 191 TC 19 Z9 44 U1 1 U2 4 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0070-2153 J9 CURR TOP DEV BIOL PY 1996 VL 32 BP 139 EP 174 PG 36 WC Developmental Biology SC Developmental Biology GA BF07L UT WOS:A1996BF07L00005 PM 8929668 ER PT J AU Wise, C Welcsh, P Ashley, J Barnes, B Buckler, A Clines, G DelMastro, R Efstradiatis, A Fischer, S Gallardo, T Haber, D Kirkpatrick, H Minna, J OsborneLawrence, S Soares, MB Spillman, M Thai, T Tomlinson, G Warburton, D Lovett, M Bowcock, A AF Wise, C Welcsh, P Ashley, J Barnes, B Buckler, A Clines, G DelMastro, R Efstradiatis, A Fischer, S Gallardo, T Haber, D Kirkpatrick, H Minna, J OsborneLawrence, S Soares, MB Spillman, M Thai, T Tomlinson, G Warburton, D Lovett, M Bowcock, A TI An annotated physical map of the BRCA2 critical region SO CYTOGENETICS AND CELL GENETICS LA English DT Meeting Abstract C1 UNIV TEXAS, SW MED CTR, DEPT BIOCHEM, DALLAS, TX 75235 USA. UNIV TEXAS, SW MED CTR, DEPT PEDIAT, DALLAS, TX USA. UNIV TEXAS, SW MED CTR, DEPT PEDIAT, DALLAS, TX USA. UNIV TEXAS, SW MED CTR, MCDERMOTT CTR, DALLAS, TX 75235 USA. UNIV TEXAS, SW MED CTR, SIMMONS CANC CTR, DALLAS, TX 75235 USA. MASSACHUSETTS GEN HOSP, CTR NEUROGENET, BOSTON, MA 02114 USA. COLUMBIA UNIV COLL PHYS & SURG, CTR HUMAN GENOME, NEW YORK, NY 10032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0301-0171 J9 CYTOGENET CELL GENET JI Cytogenet. Cell Genet. PY 1996 VL 75 IS 2-3 BP 110 EP 110 PG 1 WC Cell Biology; Genetics & Heredity SC Cell Biology; Genetics & Heredity GA WG954 UT WOS:A1996WG95400019 ER PT J AU Shows, TB Alders, M Bennett, S Burbee, D Cartwright, P Chandrasekharappa, S Cooper, P Courseaux, A Davies, C Devignes, MD Devilee, P Elliott, R Evans, G Fantes, J Garner, H Gaudray, P Gerhard, DS Gessler, M Higgins, M Hummerich, H James, M Lagercrantz, J Litt, M Little, P Mannens, M Munroe, D Nowak, N OBrien, S Parker, N Perlin, M Reid, L Richard, C Sawicki, M Swallow, D Thakker, R vanHeyningen, V vanSchothorst, E Vorechovsky, I Wadelius, C Weber, B Zabel, B AF Shows, TB Alders, M Bennett, S Burbee, D Cartwright, P Chandrasekharappa, S Cooper, P Courseaux, A Davies, C Devignes, MD Devilee, P Elliott, R Evans, G Fantes, J Garner, H Gaudray, P Gerhard, DS Gessler, M Higgins, M Hummerich, H James, M Lagercrantz, J Litt, M Little, P Mannens, M Munroe, D Nowak, N OBrien, S Parker, N Perlin, M Reid, L Richard, C Sawicki, M Swallow, D Thakker, R vanHeyningen, V vanSchothorst, E Vorechovsky, I Wadelius, C Weber, B Zabel, B TI Report of the fifth international workshop on human chromosome 11 mapping (1996) SO CYTOGENETICS AND CELL GENETICS LA English DT Editorial Material ID FAMILIAL HYPERTROPHIC CARDIOMYOPATHY; PROTEIN-C GENE; SUSCEPTIBILITY LOCUS; HUMAN GENOME; MAP; LOCALIZATION; REGION; SITE; HUMAN-CHROMOSOME-11; SEQUENCE C1 UNIV AMSTERDAM,ACAD MED CTR,NL-1105 AZ AMSTERDAM,NETHERLANDS. UNIV OXFORD,WELLCOME TRUST CTR HUMAN GENET,OXFORD OX3 7BN,ENGLAND. UNIV TEXAS,SW MED CTR,DALLAS,TX 75235. UNIV UTAH,DEPT HUMAN GENET,SALT LAKE CITY,UT 84112. NCHGR,LGT,NIH,BETHESDA,MD 20892. FAC MED,LGMCH,CNRS URA 1462,F-06717 NICE 2,FRANCE. CNRS UPR 420,F-94801 VILLEJUIF,FRANCE. LEIDEN UNIV,DEPT HUMAN GENET,NL-2333 AL LEIDEN,NETHERLANDS. ROSWELL PK CANC INST,DEPT MOL & CELL BIOL,BUFFALO,NY 14263. WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND. FAC MED NICE,CNRS URA 1462,F-06107 NICE 2,FRANCE. WASHINGTON UNIV,SCH MED,DEPT GENET,ST LOUIS,MO 63110. THEODOR BOVERI INST BIOWISSENSCH,LEHRSTUHL PHYSIOL CHEM,D-97074 WURZBURG,GERMANY. UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOCHEM,LONDON SW7 2AZ,ENGLAND. NUFFIELD ORTHOPAED CTR,WELLCOME TRUST CTR HUMAN GENET,OXFORD OX3 7LD,ENGLAND. KAROLINSKA HOSP,DEPT CLIN GENET,S-10401 STOCKHOLM,SWEDEN. OREGON HLTH SCI UNIV,DEPT BIOCHEM,PORTLAND,OR 97201. OREGON HLTH SCI UNIV,DEPT MED GENET,PORTLAND,OR 97201. MIT,CTR CANC RES,CAMBRIDGE,MA 02139. NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702. ROYAL CHILDRENS HOSP,DEPT HAEMATOL ONCOL,PARKVILLE,VIC 3052,AUSTRALIA. CARNEGIE MELLON UNIV,DEPT COMP SCI,PITTSBURGH,PA 15213. UNIV N CAROLINA,LINEBERGER CANC CTR,CHAPEL HILL,NC 27599. UNIV PITTSBURGH,WESTERN PSYCHIAT INST & CLIN,DEPT PSYCHIAT,PITTSBURGH,PA 15213. W LOS ANGELES VET AFFAIRS MED CTR,DEPT GEN SURG,LOS ANGELES,CA 90073. UNIV LONDON UNIV COLL,GALTON LAB,MRC,HUMAN BIOCHEM GENET UNIT,LONDON NW1 2HE,ENGLAND. HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,MRC,MOL ENDOCRINOL GRP,LONDON W12 0NN,ENGLAND. LEIDEN UNIV,RUL,LEIDEN,NETHERLANDS. KAROLINSKA INST,NOVUM,DEPT BIOSCI,S-14157 HUDDINGE,SWEDEN. UNIV UPPSALA HOSP,S-75185 UPPSALA,SWEDEN. BIOCTR,INST HUMAN GENET,D-97074 WURZBURG,GERMANY. UNIV MAINZ,DEPT PEDIAT,D-55101 MAINZ,GERMANY. RP Shows, TB (reprint author), ROSWELL PK CANC INST,DEPT HUMAN GENET,BUFFALO,NY 14263, USA. RI van Heyningen, Veronica/B-8039-2008 OI van Heyningen, Veronica/0000-0003-0359-0141 NR 42 TC 1 Z9 1 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0301-0171 J9 CYTOGENET CELL GENET JI Cytogenet. Cell Genet. PY 1996 VL 74 IS 1-2 BP 2 EP 52 PG 51 WC Cell Biology; Genetics & Heredity SC Cell Biology; Genetics & Heredity GA VN347 UT WOS:A1996VN34700001 ER PT J AU Chung, WK PowerKehoe, L Gusella, JF Conneally, PM Wexler, NS Leibel, RL AF Chung, WK PowerKehoe, L Gusella, JF Conneally, PM Wexler, NS Leibel, RL TI Genetic map of 1p31 in region homologous to midmurine chromosome 4 SO CYTOGENETICS AND CELL GENETICS LA English DT Meeting Abstract C1 ROCKEFELLER UNIV,NEW YORK,NY 10021. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. INDIANA UNIV,SCH MED,INDIANAPOLIS,IN. COLUMBIA UNIV COLL PHYS & SURG,NEW YORK,NY 10032. NR 0 TC 0 Z9 0 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0301-0171 J9 CYTOGENET CELL GENET JI Cytogenet. Cell Genet. PY 1996 VL 72 IS 2-3 BP 146 EP 146 PG 1 WC Cell Biology; Genetics & Heredity SC Cell Biology; Genetics & Heredity GA TX310 UT WOS:A1996TX31000008 ER PT B AU Stashenko, P Wang, CY Teles, R TaniIshii, N AF Stashenko, P Wang, CY Teles, R TaniIshii, N BE Shimono, M Maeda, T Suda, H Takahashi, K TI Immunopathologic mechanisms of pulpal and periapical destruction and protection SO DENTIN/PULP COMPLEX LA English DT Proceedings Paper CT International Conference on Dentin/Pulp Complex 1995 / International Meeting on Clinical Topics of Dentin/Pulp Complex CY JUL 01-04, 1995 CL CHIBA, JAPAN SP Int Assoc Dent Res Pulp Biol Grp, Japanese Assoc Dent Sci, Japanese Assoc Oral Biol, Japanese Assoc Dent Res AB Bacterial infections of the dental pulp result in pulpal tissue destruction and periapical bone resorption. To investigate the immunopathology of these phenomena, studies from this laboratory have employed rodent models of pulp exposure and infection from the oral environment. Such infections elicited a local 'mixed type' immune response in both the pulp and periapex, which consisted of large numbers of T cells and polymorphonuclear leukocytes (PMNs), and smaller numbers of B cells, monocytes, and plasma cells. Extracts of periapical tissues contained bone resorbing activity, which correlated quantitatively with lesion development. Bone resorptive cytokines interleukin-1 alpha(IL-1 alpha) and tumor necrosis factor a (TNF alpha), but not IL-1 beta or TNF beta, were strongly expressed in pulp and periapical lesions, as determined by immunohistochemistry, PCR, RNase protection assays, and in situ hybridization. Of interest, IL-1 alpha and TNF alpha were expressed by both infiltrating macrophages and PMNs, and by resident connective tissue cells such as fibroblasts, endothelial cells, osteoblasts, and osteoclasts. As determined by antibody neutralization, biochemical fractionation, and in vivo inhibition studies with IL-1 receptor antagonist, IL-1 alpha constituted the most important bone resorptive mediator, while TNF alpha played only a minor role, a consequence of its lower resorptive potency. The role of the specific vs. non-specific arms of the immune response in pulpal and periapical resistance to infection was also investigated. Periapical lesion development was determined in severe combined immunodeficient RAG2 knockout mice which lack T and B lymphocytes and fail to generate either cell-mediated or humoral responses. Following equalization of infecting pathogens, RAG;! mice exhibited similar amounts of localized periapical bone resorption compared to immunologically-intact controls. However, RAG2 mice experienced a greater incidence of disseminated infection and mortality, suggesting that specific T and/or B cell responses may help to localize endodontic infections to the root canal space. Enhancement of the non-specific response by the biological response modifier PGG glucan resulted in increased pulpal infection resistance and decreased destruction. Taken together, these studies suggest that both specific and non-specific immune mechanisms play important protective roles in pulpal infection resistance. RP Stashenko, P (reprint author), FORSYTH DENT CTR,140 FENWAY,BOSTON,MA 02115, USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU QUINTESSENCE PUBL CO INC PI CAROL STREAM PA 551 N KIMBERLY DR, CAROL STREAM, IL 60188-1881 BN 4-87417-522-8 PY 1996 BP 72 EP 78 PG 7 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA BG73N UT WOS:A1996BG73N00011 ER PT J AU Taylor, CR AF Taylor, CR TI Measurement of actinic erythema in healthy subjects and in subjects with polymorphous light eruption using a tristimulus colorimeter SO DERMATOLOGY LA English DT Article DE actinic erythema; polymorphous light eruption; tristimulus colorimeter RP Taylor, CR (reprint author), MASSACHUSETTS GEN HOSP,BARTLETT HALL,ROOM 414,32 FRUIT ST,BOSTON,MA 02114, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1018-8665 J9 DERMATOLOGY JI Dermatology PY 1996 VL 193 IS 2 BP 155 EP 155 PG 1 WC Dermatology SC Dermatology GA VH955 UT WOS:A1996VH95500021 PM 8884159 ER PT S AU Sodroski, J Wyatt, R Olshevsky, U Olshevsky, V Moore, J AF Sodroski, J Wyatt, R Olshevsky, U Olshevsky, V Moore, J BE Giraldo, G Bolognesi, DP Salvatore, M BethGiraldo, E TI Conformation of the HIV-1 gp120 envelope glycoprotein SO DEVELOPMENT AND APPLICATIONS OF VACCINES AND GENE THERAPY IN AIDS SE ANTIBIOTICS AND CHEMOTHERAPY LA English DT Proceedings Paper CT International Workshop on the Development and Applications of Vaccines and Gene Therapy in AIDS CY JUN 15-16, 1995 CL NAPLES, ITALY SP Ist Nazl Tumori, Fdn G Pascale Naples, Lega Italiana Lotta Tumori, Commiss European Communities, Italian Minist Hlth, Italian Assoc AIDS, Assessorato Sanita, Campania ID VARIABLE REGIONS RP Sodroski, J (reprint author), HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DEPT PATHOL,44 BINNEY ST,BOSTON,MA 02115, USA. NR 7 TC 7 Z9 7 U1 0 U2 0 PU KARGER PI BASEL PA POSTFACH, CH-4009 BASEL, SWITZERLAND SN 0066-4758 BN 3-8055-6256-X J9 ANTIBIOT CHEMOTHER PY 1996 VL 48 BP 184 EP 187 PG 4 WC Infectious Diseases; Medicine, Research & Experimental; Pharmacology & Pharmacy SC Infectious Diseases; Research & Experimental Medicine; Pharmacology & Pharmacy GA BF56F UT WOS:A1996BF56F00025 PM 8726523 ER PT J AU Song, HK Sawyer, RH AF Song, HK Sawyer, RH TI Dorsal dermis of the scaleless (sc/sc) embryo directs normal feather pattern formation until day 8 of development SO DEVELOPMENTAL DYNAMICS LA English DT Article DE pattern formation; feather induction; skin development; scaleless (sc/sc) chicken; epidermal dermal interactions ID DEVELOPING AVIAN SCALES; COLLAGEN TYPE-I; CHICK-EMBRYO; IMMUNOFLUORESCENT LOCALIZATION; CELL-PROLIFERATION; RETINOIC ACID; MORPHOGENESIS; SKIN; EPIDERMIS; INDUCTION AB We have examined the ability of the scaleless (sc/sc) backskin dermis (6 to 16 days of incubation) to regulate pattern formation using the presumptive scutate scale epidermis from 11-day normal embryos as the responding tissue. Prior to 8 days of incubation the sc/sc backskin dermis is able to induce hexagonally patterned and uniformly oriented feather germs in normal epidermis. This ability is lost during day 8 and follows a central to lateral gradient. Such gradients are characteristic of normal feather development in the spinal tract. We discuss the change in the inductive ability of the sc/sc dermis in relation to the stabilization of the feather pattern, which occurs all at once throughout the dorsal dermis at 7.5-8 days of development, After day 8 until day 10, the sc/sc backskin dermis only supports the formation of sporadic, unpatterned feather germs; thereafter it will not support feather formation. (C) 1996 Wiley-Liss, Inc. C1 UNIV S CAROLINA,DEPT BIOL SCI,COLUMBIA,SC 29208. MASSACHUSETTS GEN HOSP,CUTANEOUS BIOL RES CTR,BOSTON,MA 02129. NR 47 TC 18 Z9 18 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1058-8388 J9 DEV DYNAM JI Dev. Dyn. PD JAN PY 1996 VL 205 IS 1 BP 82 EP 91 PG 10 WC Anatomy & Morphology; Developmental Biology SC Anatomy & Morphology; Developmental Biology GA TN085 UT WOS:A1996TN08500008 PM 8770554 ER PT J AU Buonanno, A Rosenthal, N AF Buonanno, A Rosenthal, N TI Molecular control of muscle diversity and plasticity SO DEVELOPMENTAL GENETICS LA English DT Review ID TROPONIN-I GENE; MYOGENIC REGULATORY GENES; ENHANCER-BINDING FACTOR; ADULT SKELETAL-MUSCLE; LIGHT CHAIN ENHANCER; FAST-TWITCH MUSCLE; TRANSGENIC MICE; FIBER-TYPE; TRANSCRIPTION FACTOR; MYOD FAMILY C1 MASSACHUSETTS GEN HOSP EAST,CARDIOVASC RES CTR,BOSTON,MA. RP Buonanno, A (reprint author), NIH,BLDG 49,ROOM 5A-38,BETHESDA,MD 20892, USA. NR 140 TC 50 Z9 52 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0192-253X J9 DEV GENET JI Dev. Genet. PY 1996 VL 19 IS 2 BP 95 EP 107 DI 10.1002/(SICI)1520-6408(1996)19:2<95::AID-DVG1>3.0.CO;2-V PG 13 WC Developmental Biology; Genetics & Heredity SC Developmental Biology; Genetics & Heredity GA VQ330 UT WOS:A1996VQ33000001 PM 8900042 ER PT J AU Dominov, JA Miller, JB AF Dominov, JA Miller, JB TI POU homeodomain genes and myogenesis SO DEVELOPMENTAL GENETICS LA English DT Article DE POU homeodomain; Brn-4; Emb; Oct-1 transcription factor; mouse embryo; skeletal muscle; myogenesis ID CREATINE-KINASE ENHANCER; HELIX-LOOP-HELIX; TRANSCRIPTION FACTOR; MUSCLE DIFFERENTIATION; DEVELOPING BRAIN; BINDING-FACTOR; DOMAIN GENE; PAX-3 EXPRESSION; OCT-1 CONTAINS; MOUSE AB We show that members of the POU homeodomain family are among the transcription factors expressed in developing mouse skeletal muscle. From a cDNA library prepared from fetal muscle mRNA, we cloned a cDNA identical to that of Brn-4, a POU class ill gene previously cloned from neural tissues. In limb muscle, we found that Brn-4 mRNA expression was highest at embryonic days 15-18, declined after birth, and was undetectable in adults. The mRNAs of two additional POU genes, Emb (POU class VI) and Oct-1 (POU class II), were also expressed in developing muscle and, unlike Brn-4, continued to be expressed in postnatal and adult muscles. In skeletal muscle, expression of Brn-4 is myogenin-dependent, because muscles from myogenin-deficient fetuses contained much less Brn-4 mRNA than muscles from myogenin-expressing littermates. In contrast, expression of Emb was the same in the presence or absence of myogenin. The distinct pattern of Brn-4 mRNA expression and its dependence on a myogenic regulatory factor suggest that Brn-4 is part of the network of interacting transcription factors that control muscle-specific gene expression during mammalian myogenesis. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,SCH MED,NEUROSCI PROGRAM,BOSTON,MA 02115. RP Dominov, JA (reprint author), MASSACHUSETTS GEN HOSP,NEUROMUSCULAR LAB,149 13TH ST,CHARLESTOWN,MA 02129, USA. NR 74 TC 16 Z9 16 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0192-253X J9 DEV GENET JI Dev. Genet. PY 1996 VL 19 IS 2 BP 108 EP 118 DI 10.1002/(SICI)1520-6408(1996)19:2<108::AID-DVG2>3.3.CO;2-M PG 11 WC Developmental Biology; Genetics & Heredity SC Developmental Biology; Genetics & Heredity GA VQ330 UT WOS:A1996VQ33000002 PM 8900043 ER PT J AU Neville, C Gonzales, D Houghton, L McGrew, MJ Rosenthal, N AF Neville, C Gonzales, D Houghton, L McGrew, MJ Rosenthal, N TI Modular elements of the MLC 1f/3f locus confer fiber-specific transcription regulation in transgenic mice SO DEVELOPMENTAL GENETICS LA English DT Article DE fast-twitch fibers; fiber type; MyoD; myosin light chain; skeletal muscle ID PROTEIN GENE-EXPRESSION; ADULT SKELETAL-MUSCLE; HEAVY-CHAIN; MYOSIN; MYOD; SEQUENCES; DIVERSITY; ENHANCER; BINDING; RAT AB The two proteins encoded by the fast alkali myosin light chain (MLC) 1f/3f locus are developmentally regulated, muscle specific, and expressed exclusively in fast-twitch fibers. Their expression is independently regulated by two separate promoters and a downstream enhancer. Previous studies showed a reporter gene directed by the rat MLC 1f promoter and MIC enhancer to exhibit correct skeletal muscle-specific expression in transgenic mice during development and to be preferentially expressed in fast-twitch Type IIB fibers [Donoghue et al., (1991) J. Cell Biol. 115:423-434]. The MIC 3f promoter also directed muscle-specific expression of a CAT reporter gene in adult transgenic mice and showed little dependence upon the enhancer. Here, we show that the MIC 3f promoter also directs transgene expression in the fast-twitch fibers of adult skeletal muscle, but almost exclusively to fiber Types IIA and IIX. MLC 3f transgene expression occurs in only a subset of the fiber types that express the endogenous locus, indicating modular elements included in the transgene confer fiber-specific transcription regulation. MyoD protein was also found to be restricted to fiber Types IIA and IIX, providing evidence for its possible role in mediating fiber-specific gene expression. (C) 1996 Wiley-liss, Inc. C1 MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,CHARLESTOWN,MA 02129. NR 25 TC 17 Z9 17 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0192-253X J9 DEV GENET JI Dev. Genet. PY 1996 VL 19 IS 2 BP 157 EP 162 DI 10.1002/(SICI)1520-6408(1996)19:2<157::AID-DVG7>3.0.CO;2-8 PG 6 WC Developmental Biology; Genetics & Heredity SC Developmental Biology; Genetics & Heredity GA VQ330 UT WOS:A1996VQ33000007 PM 8900048 ER PT J AU Heydari, AR You, SH Takahashi, R Gutsmann, A Sarge, KD Richardson, A AF Heydari, AR You, SH Takahashi, R Gutsmann, A Sarge, KD Richardson, A TI Effect of caloric restriction on the expression of heat shock protein 70 and the activation of heat shock transcription factor 1 SO DEVELOPMENTAL GENETICS LA English DT Article DE aging; caloric restriction; heat shock protein 70; heat shock transcription factor; rat; liver ID FED AD-LIBITUM; FOOD RESTRICTION; DNA-BINDING; DIETARY RESTRICTION; GENE-EXPRESSION; DIPLOID FIBROBLASTS; AGING PROCESSES; METABOLIC-RATE; MESSENGER-RNA; LIFE-SPAN AB The regulation of heat shock protein 70 (hsp70) expression is an excellent example of a cellular mechanism that has evolved to protect all living organisms from various types of physiological stresses; therefore, the reported age-related alterations in the ability of cells to express hsp70 in response to stress could seriously compromise the ability of a senescent organism in respond to changes in its environment. Because caloric restriction (CR) is the only experimental manipulation known to retard aging and increase the survival of rodents, it was of interest to analyze the effect of CR on the age-related alteration in the induction of hsp70 expression in rat hepatocytes. The effect of CR on the nuclear transcription of hsp70 gene in rat hepatocytes in response to various levels of heat shock was determined, and it was found that the age-related decline in the transcription of hsp70 at all temperatures studied was reversed by CR. Because the heat shock transcription factor (HSF) mediates the heat-induced transcription of hsp70, the effect of CR on the induction of HSF binding activity by heat shock was studied and found to arise from HSF1, which has been shown to be involved in the induction of HSF binding activity in other cell types. The age-related decrease in the induction of HSF1 binding activity in rat hepatocytes was reversed by CR, and did not appear to be due to an accumulation of inhibitory molecules with age. Interestingly, the level of HSF1 protein was significantly higher in hepatocytes isolated from old rats fed ad libitum compared to hepatocytes obtained from rats fed the CR diet even though the levels of HSF1 binding activity were lower for hepatocytes isolated from the old rats fed ad libitum. The levels of the mRNA transcript for HSF1 was not significantly altered by age or CR. Thus, the changes in HSF1 binding activity with age and CR do not arise from changes in the level of HSF1 protein available for activation. (C) 1996 Wiley-Liss, Inc. C1 AUDIE L MURPHY MEM VET ADM MED CTR, CTR GERIATR RES EDUC & CLIN, SAN ANTONIO, TX 78284 USA. UNIV TEXAS, HLTH SCI CTR, DEPT PHYSIOL, SAN ANTONIO, TX 78284 USA. UNIV KENTUCKY, DEPT BIOCHEM, LEXINGTON, KY 40536 USA. FU NIA NIH HHS [AG01188, AG01548] NR 64 TC 75 Z9 76 U1 0 U2 1 PU WILEY-LISS PI HOBOKEN PA DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0192-253X J9 DEV GENET JI Dev. Genet. PY 1996 VL 18 IS 2 BP 114 EP 124 PG 11 WC Developmental Biology; Genetics & Heredity SC Developmental Biology; Genetics & Heredity GA UD087 UT WOS:A1996UD08700004 PM 8934873 ER PT J AU Skelly, RH Schuppin, GT Ishihara, H Oka, Y Rhodes, CJ AF Skelly, RH Schuppin, GT Ishihara, H Oka, Y Rhodes, CJ TI Glucose-regulated translational control of proinsulin biosynthesis with that of the proinsulin endopeptidases PC2 and PC3 in the insulin-producing MIN6 cell line SO DIABETES LA English DT Article ID CYCLIC-AMP; MESSENGER-RNA; BETA-CELL; MOUSE ISLETS; SECRETION; RELEASE; PROTEINS; THAPSIGARGIN; ESTABLISHMENT; RECOGNITION AB In the short term (<2 h), proinsulin biosynthesis is predominately glucose regulated at the translational level; however, the details at the molecular level behind this mechanism are not well defined, One of the major hindrances for gaining a better understanding of the proinsulin biosynthetic mechanism has been a lack of au abundant source of beta-cells that express a phenotype of regulated proinsulin biosynthesis in the appropriate 2.8-16.7 mmol/l glucose range as defined in normal pancreatic islets, In this study, we demonstrate that in the MIN6 cell line, specific glucose-regulated translational control of proinsulin biosynthesis is present in the appropriate glucose concentration range, In addition to that of proinsulin, the biosynthesis of the two proinsulin conversion endopeptidases, PC2 and PC3, was coordinately glucose regulated in MING cells, whereas that of the exopeptidase, carboxypeptidase H, was unaffected by glucose, Proinsulin, PC2, and PC3 biosynthesis was specifically stimulated over that of total MIN6 cell protein synthesis above a threshold of 4 mmol/l glucose that reached a maximum rate between 8 and 10 mmol/l glucose, Glucose-induced proinsulin, PC2, and PC3 biosynthesis was rapid (occurring after a 20-min lag period but reaching a maximum by 60 min), unaffected by the presence of actinomycin D; and in parallel experiments, stimulatory glucose concentrations did not alter MING cell total preproinsulin, PC2, or PC3 mRNA levels. Thus, short-term (<2 h) glucose stimulation of proinsulin, PC2, and PC3 biosynthesis in MIN6 cells, Like that in isolated islets, was mediated at the translational level, Intracellular signals for mediating glucose-stimulated proinsulin PC2 and PC3 biosynthesis translation in MING cells also appeared to be similar to those in pancreatic islets, requiring glucose metabolism and a supporting role for protein kinase A, However, protein kinase C or a Ca2+-dependent protein kinase did not appear to be required for glucose-regulated proinsulin biosynthesis in MING cells, as in islets, MIN6 cells are the first beta-cell line that indicate glucose-regulated proinsulin biosynthesis translation essentially identical to that in differentiated islet beta-cells and will be an important experimental model to better define the mechanism of proinsulin biosynthesis in detail. C1 BRIGHAM & WOMENS HOSP,EP JOSLIN RES LAB,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,JOSLIN DIABET CTR,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BOSTON,MA. UNIV TOKYO,DEPT INTERNAL MED 3,TOKYO 113,JAPAN. UNIV TOKYO,FAC MED,TOKYO 113,JAPAN. FU NIDDK NIH HHS [DK 307260-18, DK 36836-09] NR 50 TC 57 Z9 60 U1 0 U2 5 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0012-1797 J9 DIABETES JI Diabetes PD JAN PY 1996 VL 45 IS 1 BP 37 EP 43 DI 10.2337/diabetes.45.1.37 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TM677 UT WOS:A1996TM67700005 PM 8522057 ER PT J AU Mayfield, RK Shimojo, N Jaffa, AA AF Mayfield, RK Shimojo, N Jaffa, AA TI Skeletal muscle kallikrein - Potential role in metabolic regulation SO DIABETES LA English DT Article; Proceedings Paper CT Symposium on Significance of Autocrine and Paracrine Signaling for Energy Metabolism in Contracting Skeletal and Carldiac Muscle Tissues CY SEP 03-04, 1994 CL BUHLERHOHE, GERMANY ID TISSUE KALLIKREIN; GLUCOSE-UPTAKE; KININ SYSTEM; INSULIN; RAT; BRADYKININ; KIDNEY AB Skeletal muscle glucose metabolism appears to be regulated by locally derived factors as well as by systemically circulating hormones. Local factors may be particularly important during exercise, when substrate demand can increase rapidly. Numerous studies in perfused limbs suggest that the kallikrein-kinin system may participate in the regulation of substrate delivery and utilization by skeletal muscle. Evidence also suggests that kinins mediate the increase in insulin sensitivity after administration of converting enzyme inhibitors. Tissue kallikrein has been isolated and purified from rat skeletal muscles, and its level is highest in muscle with high oxidative activity. In other tissues, kallikrein synthesis is under the influence of insulin. It has not been possible to demonstrate effects of kallikrein or kinins on glucose metabolism in isolated skeletal muscle or cardiocytes. Therefore modulation of glucose metabolism by kallikrein or kinins may only be observed in intact perfused tissues or organs. C1 MED UNIV S CAROLINA,DEPT MED,CHARLESTON,SC 29425. MED UNIV S CAROLINA,DEPT PHARMACOL,CHARLESTON,SC 29425. RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,CHARLESTON,SC. FU NCRR NIH HHS [MO1-RR-01070-19] NR 34 TC 27 Z9 27 U1 0 U2 0 PU AMER DIABETES ASSOC PI ALEXANDRIA PA 1660 DUKE ST, ALEXANDRIA, VA 22314 SN 0012-1797 J9 DIABETES JI Diabetes PD JAN PY 1996 VL 45 SU 1 BP S20 EP S23 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TQ507 UT WOS:A1996TQ50700004 PM 8529795 ER PT J AU Levkoff, S Berkman, B Balsam, A Minaker, K AF Levkoff, S Berkman, B Balsam, A Minaker, K TI Health promotion disease prevention: New directions for geriatric education SO EDUCATIONAL GERONTOLOGY LA English DT Article AB Prevention is a major concern in work with the Elderly, especially in outpatient or community settings, where the goal is often to prevent admission to hospitals or placement in long-term care institutions. Whereas hospitals provide essential care to older adults with acute care problems, many of the more commonly experienced problems-chronic disease, functional impairments, and case management issues-are handled in outpatient settings. Primary care practitioners in office-based practices and other out patient facilities need to be better prepared to address the special problems of ambulatory geriatric patients. In response to these needs, the Harvard Upper New England Geriatric Education Center has developed 10 educational modules in health promotion/disease prevention: Alzheimer's Disease in Minority Elders, Dehydration in Long-Term Care, Diabetes Control, Elder Abuse, Geriatric Nutrition, Geriatric Oncology, Geriatric Oral Health in. Long-Term Care, Incontinence in the Elderly, Injury Prevention, and Physical Activity in the Elder ly. These areas are particularly significant to primary care practitioners who serve rural and urban disadvantaged older adults. We provide an overview of the contents and methodologies of the 10 modules, going into further detail about two of them: Geriatric Oncology and Physical Activity in the Elderly. C1 HARVARD UNIV,SCH MED,DIV AGING,BOSTON,MA 02115. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. MASSACHUSETTS DEPT PUBL HLTH,BOSTON,MA 02116. RP Levkoff, S (reprint author), HARVARD UNIV,SCH MED,DEPT SOCIAL MED,643 HUNTINGTON AVE,BOSTON,MA 02115, USA. NR 18 TC 1 Z9 1 U1 1 U2 2 PU HEMISPHERE PUBL CORP PI BRISTOL PA 1900 FROST ROAD, SUITE 101, BRISTOL, PA 19007-1598 SN 0360-1277 J9 EDUC GERONTOL JI Educ. Gerontol. PD JAN-FEB PY 1996 VL 22 IS 1 BP 93 EP 104 DI 10.1080/0360127960220109 PG 12 WC Education & Educational Research; Gerontology SC Education & Educational Research; Geriatrics & Gerontology GA TT153 UT WOS:A1996TT15300009 ER PT J AU Emanuele, NV Levey, AD Li, Q Kirsteins, L VandeKar, LD AF Emanuele, NV Levey, AD Li, Q Kirsteins, L VandeKar, LD TI The impact of cocaine on plasma growth hormone levels in the adult male rat SO ENDOCRINE RESEARCH LA English DT Article ID ABUSE; BRAIN AB Little is known about the neuroendocrine impact of cocaine as it relates to pituitarygrowth hormone secretion. In these studies, in adult male rats, intracerebroventricular administration of cocaine caused a potent dose-related suppression of circulating growth hormone (GH) in animals sacrificed 15 min after drug administration. We conclude that in the adult male rat cocaine suppresses plasma GH levels. C1 US DEPT VET AFFAIRS,MED SERV,HINES,IL 60141. LOYOLA UNIV,STRITCH SCH MED,DEPT MED,MAYWOOD,IL 60153. LOYOLA UNIV,STRITCH SCH MED,DEPT PHARMACOL,MAYWOOD,IL 60153. RP Emanuele, NV (reprint author), US DEPT VET AFFAIRS,RES SERV,HINES,IL 60141, USA. FU NIDA NIH HHS [DA04865] NR 15 TC 1 Z9 1 U1 0 U2 0 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 0743-5800 J9 ENDOCR RES JI Endocr. Res. PY 1996 VL 22 IS 2 BP 139 EP 145 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA UT929 UT WOS:A1996UT92900004 PM 8799693 ER PT J AU Teixeira, J He, WW Shah, PC Morikawa, N Lee, MM Catlin, EA Hudson, PL Wing, J Maclaughlin, DT Donahoe, PK AF Teixeira, J He, WW Shah, PC Morikawa, N Lee, MM Catlin, EA Hudson, PL Wing, J Maclaughlin, DT Donahoe, PK TI Developmental expression of a candidate Mullerian inhibiting substance type II receptor SO ENDOCRINOLOGY LA English DT Article ID SERINE THREONINE KINASE; ACTIVIN RECEPTOR; RIBONUCLEIC-ACID; CLONING; DOMAIN; FAMILY; GENES; CELLS AB We have isolated a candidate Mullerian inhibiting substance (MIS) type II receptor complementary DNA from an embryonic rat urogenital ridge library and have studied its binding to MIS, its developmental pattern of expression and tissue distribution. By in situ hybridization with a full-length riboprobe, the receptor is expressed in the mesenchymal cells surrounding the Mullerian duct at embryonic days 14, 15, and 16 and in tubular and follicular structures of the rat fetal gonads. Expression of the messenger RNA was also seen in the granulosa cells and seminiferous tubules of pubertal gonads. Northern analysis revealed that the MIS type II receptor messenger RNA is highly expressed in embryonic, pubertal, and adult testes and ovaries, as well as in the gravid uterus. The timing of expression in the gonads of both sexes was also analyzed by Northern analyses that showed high levels of expression at the time of Mullerian duct regression, much lower levels neonatally and prepubertally and then increased expression again with sexual maturation. The tissue and developmental specificity of expression of this receptor, which make it likely that this is the functional MIS type II receptor, can be used to advantage in therapeutic targeting strategies and to decipher the function of MIS in the gonads. C1 MASSACHUSETTS GEN HOSP, DEPT SURG, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, PEDIAT SURG RES LAB, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, DEPT OBSTET & GYNECOL, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, DEPT PEDIAT, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. OI Lee, Mary/0000-0002-7204-4884 FU NCI NIH HHS [R01 CA-17393]; NICHD NIH HHS [P30 HD-28138, R01 HD-32112] NR 36 TC 102 Z9 109 U1 1 U2 10 PU ENDOCRINE SOC PI WASHINGTON PA 2055 L ST NW, SUITE 600, WASHINGTON, DC 20036 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD JAN PY 1996 VL 137 IS 1 BP 160 EP 165 DI 10.1210/en.137.1.160 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TL323 UT WOS:A1996TL32300023 PM 8536608 ER PT J AU Yu, JX Xie, LY AbouSamra, AB AF Yu, JX Xie, LY AbouSamra, AB TI Molecular cloning of a type A chicken corticotropin-releasing factor receptor with high affinity for urotensin I SO ENDOCRINOLOGY LA English DT Article ID VASOACTIVE-INTESTINAL-PEPTIDE; HORMONE-RELATED PEPTIDE; EXPRESSION CLONING; PARATHYROID-HORMONE; FUNCTIONAL EXPRESSION; CALCITONIN RECEPTOR; BRAIN; POLYPEPTIDE; CRF AB The hypothalamic-pituitary-adrenal axis is an essential physiological system in many species. CRF, the major neuropeptide regulating ACTH secretion, is highly conserved in its primary sequence. Evolutionary conservation of the CRF sequence suggests that the CRF receptor (CRF-R) itself is highly conserved. Therefore, we cloned the chicken CRF-R (cCRF-R) complementary DNA and examined its properties. The avian CRF-R complementary DNA encodes a 420-amino acid protein that is 87-88% identical to those of human, rat, and mouse. Most sequence divergence occurs in the putative signal peptide and the extracellular amino-terminus of the receptor. Five additional amino acids are inserted in the amino-terminus of the cCRF-R. When expressed in COS-7 cells, the cCRF-R binds the CRF and urotensin I radioligands with high affinities. Urotensin I competes for binding to the chicken CRF-R, expressed in COS-7 cells, with an apparent affinity 20 times higher than that of CRF. Both urotensin I and sauvagine were more effective in stimulating cAMP accumulation in COS-7 cells transfected with the cCRF-R than CRF. The effects of CRF and urotensin I on inositol phosphate accumulation were also tested. Urotensin I was as effective as CRF in stimulating inositol phosphate accumulation in COS-7 cells transfected with the cCRF-R. These data suggest that the sequence of the CRF-R is highly conserved from avian to mammalian species and that, despite its high sequence homology to the type A mammalian CRF-R, the ligand binding properties of cCRF-R are similar to those of the type B CRF-R, i.e. a higher affinity for urotensin I than for CRF. C1 MASSACHUSETTS GEN HOSP, ENDOCRINE UNIT, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. OI Abou-Samra, Abdul/0000-0001-8735-1142 NR 33 TC 63 Z9 65 U1 0 U2 2 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD JAN PY 1996 VL 137 IS 1 BP 192 EP 197 DI 10.1210/en.137.1.192 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TL323 UT WOS:A1996TL32300027 PM 8536612 ER PT J AU Schneyer, AL Hall, HA LambertMesserlian, G Wang, QF Sluss, P Crowley, WF AF Schneyer, AL Hall, HA LambertMesserlian, G Wang, QF Sluss, P Crowley, WF TI Follistatin activin complexes in human serum and follicular fluid differ immunologically and biochemically SO ENDOCRINOLOGY LA English DT Article ID GRANULOSA-CELLS INVITRO; SUPPRESSING PROTEIN FOLLISTATIN; STIMULATING-HORMONE; BINDING-PROTEIN; INDUCED DIFFERENTIATION; PITUITARY; RELEASE; INHIBIN; EXPRESSION; TISSUE AB Follistatin (FS) is the principle high affinity activin-binding protein in tissues such as the pituitary and ovary as well as in serum. In addition, the activin-binding peaks identified after gel filtration of serum or human follicular fluid (hFF) exhibited high affinity and low reversibility binding kinetics, with higher concentrations in hFF than serum. This extremely low reversibility was also observed for recombinant human follistatin 288 (rhFS288) under a variety of incubation conditions, further supporting the identification of the serum and hFF activin-binding proteins as FS. Using enhanced resolution gel filtration, immunoprecipitation with monoclonal antibodies to rhFS288, and sulfated carbohydrate binding, activin-FS complexes in hFF and serum differed. The activin-FS complex in hFF elutes at approximately 200-300 kDa, is immunoprecipitated by anti-hFS288 monoclonal antibodies, and binds to sulfate Cellufine matrix, all characteristics similar to those of recombinant human FS288. In contrast, the activin binding peak in human serum elutes at an apparent M(r) of 60-70 kDa, is not precipitated by anti-rhFS288 monoclonal antibodies, and is weakly bound by sulfate Cellufine matrix, characteristics shared by rhFS315 conditioned medium. As the forms of FS that bind sulfate-containing matrices also bind to cell surface proteoglycans, the molecular differences reported here for serum and hFF activin-binding proteins have implications for potential tissue-specific forms of FS that may well have distinct biological functions. C1 MASSACHUSETTS GEN HOSP, NATL CTR INFERTIL RES, BOSTON, MA 02214 USA. MASSACHUSETTS GEN HOSP, REPROD ENDOCRINE UNIT, BOSTON, MA 02214 USA. OI Messerlian, Geralyn/0000-0002-9440-3411 FU NICHD NIH HHS [P30-HD-23138, P30 HD028138, U01 HD044417, U54 HD029164, U54-HD-29164] NR 34 TC 52 Z9 53 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD JAN PY 1996 VL 137 IS 1 BP 240 EP 247 DI 10.1210/en.137.1.240 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TL323 UT WOS:A1996TL32300033 PM 8536619 ER PT J AU Sherman, LA Hirshman, MF Cormont, M LeMarchandBrustel, Y Goodyear, LJ AF Sherman, LA Hirshman, MF Cormont, M LeMarchandBrustel, Y Goodyear, LJ TI Differential effects of insulin and exercise on Rab4 distribution in rat skeletal muscle SO ENDOCRINOLOGY LA English DT Article ID GLUCOSE TRANSPORTER NUMBER; GTPASE-ACTIVATING PROTEIN; PLASMA-MEMBRANE; INDUCED TRANSLOCATION; ADIPOCYTES; SEC4; LOCALIZATION; CLONING; BINDING; GLUT4 AB Insulin and exercise cause the translocation of GLUT4 from an intracellular location to the plasma membrane in skeletal muscle. The purpose of this study was to determine if Rab4, a small GTP binding protein that has been implicated in the insulin-stimulated translocation of GLUT4 in adipose cells, is involved in the regulation of transporter translocation in skeletal muscle. Male rats were injected with insulin (20 U ip) or exercised on a treadmill (1 h, 20 m/min, 10% grade). Rats were killed 30 min after insulin injection or immediately after exercise, and the hind limb muscles dissected. Plasma membrane and intracellular microsomal membrane fractions were prepared, and the distribution of GLUT4 and Rab4 was determined by immunoblotting. Both insulin and exercise caused GLUT4 translocation as demonstrated by a decrease in microsomal membrane GLUT4 and an increase in plasma membrane GLUT4. In contrast, only insulin caused a decrease in Rab4 in the microsomal membranes. Rab4 was associated with GLUT4-containing vesicles isolated by immunoprecipitation. Rab4 was not detected in plasma membranes under any condition. These data demonstrate that insulin modulates the subcellular distribution of both GLUT4 and Rab4 in rat skeletal muscle, suggesting that Rab4 may play a role in the insulin-stimulated movement of GLUT4-containing vesicles. Although both insulin and exercise increase skeletal muscle glucose uptake by the translocation of GLUT4, the regulation of translocation may occur by different mechanisms. C1 BRIGHAM & WOMENS HOSP, JOSLIN DIABET CTR, DEPT MED, DIV RES, METAB SECT, BOSTON, MA 02215 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02215 USA. FAC MED NICE, INSERM, F-06107 NICE, FRANCE. FU NIAMS NIH HHS [AR-42238] NR 47 TC 54 Z9 55 U1 3 U2 3 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0013-7227 EI 1945-7170 J9 ENDOCRINOLOGY JI Endocrinology PD JAN PY 1996 VL 137 IS 1 BP 266 EP 273 DI 10.1210/en.137.1.266 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TL323 UT WOS:A1996TL32300036 PM 8536622 ER PT J AU Cornford, EM Truong, HVM Sofia, RD Kucharczyk, N AF Cornford, EM Truong, HVM Sofia, RD Kucharczyk, N TI Distribution of felbamate in brain SO EPILEPSIA LA English DT Article DE autoradiographic localization; dicarbamate anticonvulsant; CNS distribution ID TEMPORAL-LOBE EPILEPSY; POSITRON EMISSION TOMOGRAPHY; MU-OPIATE RECEPTORS AB We studied the distribution of felbamate (FBM) in rat brain using a brain imaging scanner to analyze thaw-mount autoradiographs. After intravenous injection of [C-14]FBM in rats, the autoradiographic distribution of isotope labeling patterns in brain was captured on x-ray film. Densitometric differences on the x-ray film were converted into color-code variations representing the different concentrations of FBM in regions of the brain. We demonstrated that relatively uniform concentrations of FBM were detected throughout the brain. In all brain regions examined, there were no specifically high or low concentrations of FBM. We conclude that the FBM distributes uniformly. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024. WALLACE LABS,CRANBURY,NJ. RP Cornford, EM (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,EPILEPSY CTR 691W127B,11300 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 15 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0013-9580 J9 EPILEPSIA JI Epilepsia PD JAN PY 1996 VL 37 IS 1 BP 15 EP 18 DI 10.1111/j.1528-1157.1996.tb00505.x PG 4 WC Clinical Neurology SC Neurosciences & Neurology GA TP556 UT WOS:A1996TP55600004 PM 8603617 ER PT B AU Podolsky, DK AF Podolsky, DK BE Kagnoff, MF Kiyono, H TI Regulatory peptides and integration of the intestinal epithelium in mucosal responses SO ESSENTIALS OF MUCOSAL IMMUNOLOGY LA English DT Proceedings Paper CT 8th International Congress of Mucosal Immunology CY JUN 04, 1995 CL SAN DIEGO, CA SP Falk Fdn, e V, Glaxo Inc RP Podolsky, DK (reprint author), HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,DEPT MED,GASTROINTESTINAL UNIT,BOSTON,MA 02114, USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 BN 0-12-394330-2 PY 1996 BP 101 EP 110 DI 10.1016/B978-012394330-9/50011-9 PG 10 WC Gastroenterology & Hepatology; Immunology SC Gastroenterology & Hepatology; Immunology GA BG31B UT WOS:A1996BG31B00009 ER PT B AU Casanova, JE AF Casanova, JE BE Kagnoff, MF Kiyono, H TI Structure and function of the polymeric immunoglobulin receptor in epithelial cells SO ESSENTIALS OF MUCOSAL IMMUNOLOGY LA English DT Proceedings Paper CT 8th International Congress of Mucosal Immunology CY JUN 04, 1995 CL SAN DIEGO, CA SP Falk Fdn, e V, Glaxo Inc RP Casanova, JE (reprint author), MASSACHUSETTS GEN HOSP EAST,CHARLESTOWN,MA 02129, USA. NR 0 TC 7 Z9 7 U1 3 U2 3 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 BN 0-12-394330-2 PY 1996 BP 151 EP 166 DI 10.1016/B978-012394330-9/50015-6 PG 16 WC Gastroenterology & Hepatology; Immunology SC Gastroenterology & Hepatology; Immunology GA BG31B UT WOS:A1996BG31B00013 ER PT J AU Benkov, L AF Benkov, L TI Reproduction, ethics and the law: Feminist perspectives - Callahan,JC SO ETHICS & BEHAVIOR LA English DT Book Review C1 HARVARD UNIV,SCH MED,CAMBRIDGE,MA 02138. RP Benkov, L (reprint author), DANA FARBER CANC INST,SCH LIASON PROGRAM,BOSTON,MA 02115, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU LAWRENCE ERLBAUM ASSOC INC PI MAHWAH PA 10 INDUSTRIAL AVE, MAHWAH, NJ 07430-2262 SN 1050-8422 J9 ETHICS BEHAV JI Ethics Behav. PY 1996 VL 6 IS 3 BP 265 EP 267 DI 10.1207/s15327019eb0603_8 PG 3 WC Ethics; Psychology, Multidisciplinary SC Social Sciences - Other Topics; Psychology GA WG077 UT WOS:A1996WG07700008 ER PT J AU Meier, CA AF Meier, CA TI Mechanisms of immunosuppression by glucocorticoids SO EUROPEAN JOURNAL OF ENDOCRINOLOGY LA English DT Article RP Meier, CA (reprint author), MASSACHUSETTS GEN HOSP,DIV ENDOCRINE,FRUIT ST,WEL501,BOSTON,MA 02114, USA. NR 3 TC 16 Z9 16 U1 0 U2 0 PU SCANDINAVIAN UNIVERSITY PRESS PI OSLO PA PO BOX 2959 TOYEN, JOURNAL DIVISION CUSTOMER SERVICE, N-0608 OSLO, NORWAY SN 0804-4643 J9 EUR J ENDOCRINOL JI Eur. J. Endocrinol. PD JAN PY 1996 VL 134 IS 1 BP 50 EP 50 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TR470 UT WOS:A1996TR47000009 PM 8590954 ER PT J AU Blankensteijn, JD Gertler, JP Petersen, MJ Brewster, DC Cambria, RP LaMuraglia, GM Abbott, WM AF Blankensteijn, JD Gertler, JP Petersen, MJ Brewster, DC Cambria, RP LaMuraglia, GM Abbott, WM TI Avoiding infrainguinal bypass wound complications in patients with chronic renal insufficiency: The role of the anatomic plane SO EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY LA English DT Article ID LIMB SALVAGE; DISEASE; REVASCULARIZATION; DEFINITIONS AB Objective: To study the factors leading to wound problems in patients with chronic renal insufficiency (CRI) with emphasis on subcutaneous vs. deep placement of grafts. Methods: The outcomes of patients undergoing an infrainguinal bypass with preoperative CRI (serum creatinine greater than or equal to 2.0 mg/dl) were reviewed. Surgical site infection (SSI) was classified as superficial or deep according to the Centres for Disease Control standards. Results: Forty-two patients underwent a total of 47 infrainguinal bypasses for ischaemic rest pain or tissue loss. The graft location was partially or predominantly subcutaneous in 21 limbs (Group I) and 26 grafts were positioned in the anatomic or subfascial planes (Group II). In Group I, seven early (<30 days postoperative), one intermediate (4-6 weeks postoperative), and one late (>6 weeks postoperative) SSI's were found (9/21, 43%). In three of these patients the graft was exposed and two required removal. In contrast, only two early and one intermediate SSI's (3/26, 12%) were noted in Group II (p = 0.02). A logistic regression analysis, with twelve possible covariables to wound healing, confirmed the subcutaneous location to be the only controllable factor significantly predicting SSI (relative risk = 11.6, p = 0.01). Conclusions: The infrainguinal bypass in patients with CRI is associated with a high incidence of wound complications. In our retrospective series, the presence of a vascular conduit in the subcutaneous plane was connected with a higher rate of SSI. Despite the glowing trend toward the use of the in situ bypass, CRI may represent a circumstance where deeply placed grafts should be used preferentially. C1 HARVARD UNIV, MASSACHUSETTS GEN HOSP,SCH MED,DEPT SURG, DIV VASC SURG, BOSTON, MA 02114 USA. RI Blankensteijn, Jan/Q-9291-2016 OI Blankensteijn, Jan/0000-0002-2062-8749 NR 13 TC 16 Z9 16 U1 0 U2 0 PU W B SAUNDERS CO LTD PI LONDON PA 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND SN 1078-5884 J9 EUR J VASC ENDOVASC JI Eur. J. Vasc. Endovasc. Surg. PD JAN PY 1996 VL 11 IS 1 BP 98 EP 104 DI 10.1016/S1078-5884(96)80142-0 PG 7 WC Surgery; Peripheral Vascular Disease SC Surgery; Cardiovascular System & Cardiology GA TR460 UT WOS:A1996TR46000016 PM 8564495 ER PT J AU Saini, S AF Saini, S TI Organization of research in radiology: The American model SO EUROPEAN RADIOLOGY LA English DT Article; Proceedings Paper CT 1st European Workshop of the Junior-Radiologists-Forum (JRF) of the European-Association-of-Radiology (EAR), at ECR 95 CY MAR 04, 1995 CL VIENNA, AUSTRIA SP EAR, JRF RP Saini, S (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,32 FRUIT ST,BOSTON,MA 02114, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0938-7994 J9 EUR RADIOL JI Eur. Radiol. PY 1996 VL 6 IS 2 BP S3 EP S4 PG 2 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA UE805 UT WOS:A1996UE80500026 PM 8797989 ER PT J AU Szebeni, J Sykes, M AF Szebeni, J Sykes, M TI CAG repeat mutation in the mouse IL-2 gene enables concurrent assessment of T- and B-cell-specific gene expression by Northern analysis SO EXPERIMENTAL AND CLINICAL IMMUNOGENETICS LA English DT Article DE CAG repeat; triplet repeat genes; IL-2 cDNA; class-II-associated invariant chain; T cell activation; Northern analysis ID INTERLEUKIN-2; DISTINCT AB The unique presence of CAG triplet repeats in the mouse IL-2 gene, lending the IL-2 cDNA a second specificity to MHC class II invariant chain (Ii) mRNA, coincides with another unusual genetic feature of the mouse, the lack of Ii gene upregulation in stimulated T cells. While the former anomaly allows simultaneous measurement of IL-2 and Ii mRNAs by Northern analysis, the latter condition ensures that Ii mRNA remains a T-cell-independent, specific marker of Ii positive, primarily B cell function. Thus, Northern analysis using CAG repeat containing IL-2 cDNA enables concurrent assessment of T and B cell activities in the spleen or in other mixtures of mouse lymphocytes. C1 MASSACHUSETTS GEN HOSP,TRANSPLANTAT BIOL RES CTR,CHARLESTOWN,MA. FU NIAID NIH HHS [R01AI31158] NR 7 TC 0 Z9 0 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0254-9670 J9 EXP CLIN IMMUNOGENET JI Exp. Clin. Immunogenet. PY 1996 VL 13 IS 2 BP 117 EP 119 PG 3 WC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology SC Biochemistry & Molecular Biology; Genetics & Heredity; Immunology GA WJ787 UT WOS:A1996WJ78700008 PM 9063704 ER PT J AU SchmidtErfurth, U Flotte, TJ Gragoudas, ES Schomacker, K Birngruber, R Hasan, T AF SchmidtErfurth, U Flotte, TJ Gragoudas, ES Schomacker, K Birngruber, R Hasan, T TI Benzoporphyrin-lipoprotein-mediated photodestruction of intraocular tumors SO EXPERIMENTAL EYE RESEARCH LA English DT Article DE photodynamic therapy; benzoporphyrin derivative; low-density lipoprotein; intraocular tumors, in vivo ID PHOTODYNAMIC THERAPY; PLASMA-LIPOPROTEINS; MALIGNANT-MELANOMA; CHOLESTEROL-METABOLISM; ENDOTHELIAL-CELLS; HEMATOPORPHYRIN; PHOTOSENSITIZATION; PHOTOIMMUNOTHERAPY; BIODISTRIBUTION; INVITRO AB Benzoporphyrin derivative (BPD), a sensitizer currently in clinical trials, was evaluated for the treatment of experimental Greene melanoma implanted in the rabbit iris. To improve tumor targeting, BPD was complexed with low-density lipoprotein (LDL) representing an endogenous carrier system for BPD as previously described. Twelve tumors were irradiated at a sensitizer dose of 2 mg kg(-1) body weight using a dye laser at 692 nm. Tumor responses were documented by photography, angiography and light and electron microscopy. All tumors treated with 80 J cm(-2) regressed irreversibly. The principal mechanism of tumor necrosis was thrombosis following disruption of endothelial membranes. Ultrastructure data suggested tumor cell damage, although evidence for this being the result of direct PDT-mediated tumor cell death was less clear. These data suggest that BPD-LDL may be used to improve the selectivity of photodynamic tumor therapy possibly by the increased uptake of lipoprotein-delivered sensitizer to neovascular endothelial cells. (C) 1996 Academic Press Limited C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. HARVARD UNIV,MASSACHUSETTS EYE & EAR INFIRM,SCH MED,DEPT OPHTHALMOL,RETINA SERV,BOSTON,MA. RI Birngruber, Reginald/Q-2342-2016 FU NIAMS NIH HHS [R29 AR389190-04] NR 43 TC 22 Z9 22 U1 0 U2 0 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON, ENGLAND NW1 7DX SN 0014-4835 J9 EXP EYE RES JI Exp. Eye Res. PD JAN PY 1996 VL 62 IS 1 BP 1 EP 10 DI 10.1006/exer.1996.0001 PG 10 WC Ophthalmology SC Ophthalmology GA TT612 UT WOS:A1996TT61200001 PM 8674504 ER PT J AU Ishimaru, F Potter, NS Shipp, MA AF Ishimaru, F Potter, NS Shipp, MA TI Phorbol ester-mediated regulation of CD10/neutral endopeptidase transcripts in acute lymphoblastic leukemias SO EXPERIMENTAL HEMATOLOGY LA English DT Article DE metalloproteinase; lymphoid cells; mRNA stability ID MESSENGER-RNA DEGRADATION; NEUTRAL ENDOPEPTIDASE; EPITHELIAL-CELLS; EXPRESSION; CD10; MODULATION; ANTIGEN; LUNG; DIFFERENTIATION; PROTEIN AB The cell-surface zinc metalloproteinase CD10/neutral endopeptidase 24.11 (CD10/NEP) hydrolyzes a variety of peptide substrates and regulates related peptide-mediated cellular responses. Because the enzyme functions as part of a peptide regulatory loop, the fact that CD10/NEP itself varies with cellular activation is of considerable interest. In hematopoietic and nonhematopoietic cell types, the levels of CD10/NEP protein and enzymatic activity correlate with transcript abundance. For these reasons, we investigated the regulation of CD10/NEP transcripts in the phorbol ester-treated acute lymphoblastic leukemia cell line, REH. When REH cells are treated with phorbol myristate acetate (PMA), CD10/NEP transcripts rapidly decrease in a labile protein-dependent manner. PMA has a modest effect on CD10/NEP transcription and significantly reduces CD10/NEP mRNA stability. Of note, the predicted secondary structure of the CD10/NEP 3' untranslated region includes several stem loop structures that may affect the stability of CD10/NEP transcripts. C1 DANA FARBER CANC INST,DEPT MED,DIV HEMATOL MALIGNANCIES,BOSTON,MA 02215. HARVARD UNIV,SCH MED,BOSTON,MA 02115. NR 28 TC 15 Z9 15 U1 0 U2 0 PU CARDEN JENNINGS PUBL CO LTD PI CHARLOTTESVILLE PA BLAKE CTR, STE 200, 1224 W MAIN ST, CHARLOTTESVILLE, VA 22903 SN 0301-472X J9 EXP HEMATOL JI Exp. Hematol. PD JAN PY 1996 VL 24 IS 1 BP 43 EP 48 PG 6 WC Hematology; Medicine, Research & Experimental SC Hematology; Research & Experimental Medicine GA TN334 UT WOS:A1996TN33400007 PM 8536791 ER PT J AU Heiligenhaus, A Dutt, JE Foster, CS AF Heiligenhaus, A Dutt, JE Foster, CS TI Histology and immunopathology of systemic lupus erythematosus affecting the conjunctiva SO EYE LA English DT Article DE conjunctiva; systemic lupus erythematosus; immunopathology; cicatrising conjunctivitis ID OCULAR FINDINGS; T-CELLS; DISEASE; ANTIGEN; RETINOPATHY; SUBSETS; INVITRO AB Systemic lupus erythematosus (SLE) is an autoimmune disease occasionally involving the conjunctiva, sclera or cornea, The immunopathology of the active epibulbar lesions has not been studied in detail, Conjunctival biopsies from 11 SLE patients with active epibulbar lesions and from 12 age-matched individuals undergoing cataract surgery were analysed by light microscopy, immunofluorescence and immunoperoxidase techniques, SLE patients presented with scleritis (3 cases), peripheral ulcerative keratitis (5 cases) or progressive cicatrising conjunctivitis (5 cases). Histologically, SLE specimens showed moderate subepithelial and perivascular mononuclear cell infiltration or granuloma formation in the substantia propria, and squamous metaplasia; thrombosis was not seen, Immunoreactant deposition was present at the epithelial basement membrane in 4 of 5 cases with cicatrising conjunctivitis, Vascular immunodeposits were detected in 4 cases, The epithelium showed increased T helper cells (CD4+), granulocytes and natural killer cells (CD67+), dendritic cells (CD1+), and an increase in HLA-DR expression compared with normal tissue, In the substantia propria, B cells (CD22+), macrophages (CD14+), dendritic cells, activated T cells (CD25+, CD3+), the T helper (CD4+)/T suppressor (CD8+) ratio and HLA-DR expression were all increased. These observations suggest that the rare epibulbar manifestations in SLE result from immune-complex-mediated reactions. C1 HARVARD UNIV,MASSACHUSETTS EYE & EAR INFIRM,SCH MED,HILLES IMMUNOL LAB,IMMUNOL & UVEITIS SERV,BOSTON,MA. RP Heiligenhaus, A (reprint author), UNIV ESSEN GESAMTHSCH,DEPT OPHTHALMOL,HUFELANDSTR 55,D-45122 ESSEN,GERMANY. NR 39 TC 21 Z9 21 U1 0 U2 1 PU ROYAL COLL OPHTHALMOLOGISTS PI LONDON PA 17 CORNWALL TERRACE, LONDON, ENGLAND NW1 4QW SN 0950-222X J9 EYE JI Eye PY 1996 VL 10 BP 425 EP 432 PN 4 PG 8 WC Ophthalmology SC Ophthalmology GA VD865 UT WOS:A1996VD86500004 PM 8944091 ER PT J AU Tomera, JF AF Tomera, JF TI Interaction of lipopolysaccharide endotoxin produced from Escherichia coli with D-tubocurarine at the nicotinic(2) receptor and adenosine 3':5' cyclic monophosphate during physiological contraction in skeletal muscle SO FOOD AND CHEMICAL TOXICOLOGY LA English DT Article ID SIGNAL-TRANSDUCTION MECHANISMS; H-3 POLYINOSITOL PHOSPHATES; SURFACE AREA BURN; MULTIPLE-REGRESSION; MULTIVARIATE INFLUENCE; MUSCARINIC RECEPTOR; INOSITOL PHOSPHATES; CROSS-TALK; TRAUMA; INHIBITION AB In this report the murine model of endotoxicosis was used to evaluate hyposensitivity to the neuromuscular relaxant D-tubocurarine (dTC). This hyposensitivity was expressed in terms of a decreased potency to dTC. A rightward shift of the dose-response curve due to endotoxin was observed. Mice were subjected to cumulative intraperitoneal doses of Escherichia coli endotoxin over a 2-wk period. The interaction between endotoxin and dTC was examined during an acute (1 wk) and chronic (2 wk) period of endotoxicosis. Muscle twitch analyses were performed and samples of gastrocnemius muscle were assayed for adenosine 3':5' cyclic monophosphate (cAMP) by [I-125]radioimmunoassay. A parallel shift in the dose-response curve occurred in the endotoxin group subjected to doses corresponding to one-third the dose evoking 50% lethality for 2 wk. Both skeletal muscle tension and cAMP levels decreased as cumulative endotoxin doses increased. A relationship between decreasing cAMP levels and increasing dTC and effective dose required to achieve 50% muscle paralysis values was thought to be evoked by the agonistic activity of E.coli endotoxin leading to desensitization of adenylate cyclase. The perturbations of the classical second messenger cAMP system by endotoxin may be responsible for the skeletal muscle dysfunction observed in immunocompromised patients. C1 SHRINERS BURN INST,ANESTHESIA SERV,CLIN PHARMACOL LAB,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT ANESTHESIOL,BOSTON,MA 02114. NR 44 TC 0 Z9 0 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0278-6915 J9 FOOD CHEM TOXICOL JI Food Chem. Toxicol. PD JAN PY 1996 VL 34 IS 1 BP 63 EP 72 DI 10.1016/0278-6915(95)00086-0 PG 10 WC Food Science & Technology; Toxicology SC Food Science & Technology; Toxicology GA TV150 UT WOS:A1996TV15000009 PM 8603799 ER PT J AU Lambert, CR Black, HS Truscott, TG AF Lambert, CR Black, HS Truscott, TG TI Reactivity of butylated hydroxytoluene SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE butylated hydroxytoluene; pulse radiolysis; free radicals ID ULTRAVIOLET-LIGHT; AQUEOUS-SOLUTION; RADICALS; PHENOLS; BHT AB Butylated hydroxytoluene (BHT) is a synthetic antioxidant that is widely used as an additive in foodstuffs to prevent spoiling. The physical-chemical properties of BHT and many related phenols have been examined previously although the mechanisms by which it exerts its antioxidant properties are poorly understood. The reactivity of BHT with singlet oxygen [O-2((1) Delta)(g)] and a number of radical species has been examined using the techniques of time resolved luminescence and pulse radiolysis. In benzene solution BHT reacted with O-2((1) Delta(g)) at a bimolecular rate constant of 1.3 x 10(6) M(-1)s(-1). The one-electron oxidized, phenoxyl type BHT radical was generated using pulse radiolysis and the absorption spectrum showed a maximum at 400 nm. BHT reacts slowly with many radical species and upper limits for the bimolecular rate constant for reaction with several electron transfer processes are presented. The antioxidant role of BHT is discussed in terms of its reactivity, localization, and stability. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. BAYLOR COLL MED,HOUSTON,TX 77030. VET AFFAIRS MED CTR,PHOTOBIOCHEM LAB,HOUSTON,TX 77030. UNIV KEELE,DEPT CHEM,KEELE ST5 5BG,STAFFS,ENGLAND. NR 26 TC 27 Z9 27 U1 2 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PY 1996 VL 21 IS 3 BP 395 EP 400 DI 10.1016/0891-5849(96)00050-0 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA VB873 UT WOS:A1996VB87300017 PM 8855452 ER PT J AU Oroskar, AA Lambert, C Peak, MJ AF Oroskar, AA Lambert, C Peak, MJ TI Effects of hydroxyl radical scavengers on relaxation of supercoiled DNA by aminomethyl-trimethyl-psoralen and monochromatic UVA photons SO FREE RADICAL BIOLOGY AND MEDICINE LA English DT Article DE AMT; WA; DNA single-strand breaks; hydroxyl radical scavengers; free radicals ID SINGLET OXYGEN; AQUEOUS-SOLUTION; ACTION SPECTRUM; ULTRAVIOLET; RADIATION; FUROCOUMARINS; INACTIVATION; BREAKAGE; ABSENCE AB The ability of scavengers of hydroxyl radical (OH radical) to modulate the photosensitized relaxation (induction of the first single-strand break) of supercoiled plasmid DNA with UVA photoactivated 4'-aminomethyl-4,5',8-trimethylpsoralen was examined by comparing the dose reduction factor (DRF: the ratio of fluence required to induce the same degree of relaxation in the absence to the presence of OH radical scavengers). The addition of mannitol, azide, acetate, or formate at concentrations inversely proportional to the value of the rate constants for the scavenging of OH radicals partially attenuated the supercoiled DNA relaxation. The degrees of protection afforded by the four scavengers in the presence of AMT photoactivated by either 334 nm or 365 nm monochromatic photons were similar, giving an average DRF of about 0.25 in all cases. Given the diverse chemical nature of the scavengers and their wide range of concentrations utilized, these findings are evidence for the involvement of a Type I photosensitization in the induction of DNA single-strand breaks by photoactivated AMT. C1 ARGONNE NATL LAB,CTR MECH BIOL & BIOTECHNOL,ARGONNE,IL 60439. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,BOSTON,MA. NR 27 TC 11 Z9 11 U1 1 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0891-5849 J9 FREE RADICAL BIO MED JI Free Radic. Biol. Med. PY 1996 VL 20 IS 5 BP 751 EP 756 DI 10.1016/0891-5849(95)02158-2 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA UD144 UT WOS:A1996UD14400011 PM 8721619 ER PT S AU Kazemi, H Beagle, J Maher, T Hoop, B AF Kazemi, H Beagle, J Maher, T Hoop, B BE Zapata, P Eyzaguirre, C Torrance, RW TI Afferent input from peripheral chemoreceptors in response to hypoxia and amino acid neurotransmitter generation in the medulla SO FRONTIERS IN ARTERIAL CHEMORECEPTION SE Advances in Experimental Medicine and Biology LA English DT Proceedings Paper CT XIIIth International Symposium on Arterial Chemoreceptors CY MAR 25-29, 1996 CL CATHOLIC UNIV CHILE, EXPTL CTR, SANTIAGO, CHILE SP Int Soc Arterial Chemorecept, Catholic Univ Chile, Natl Commiss Sci & Technol Res, Chile, Andes Fdn, 3rd World Acad Sci, Merck, Chile, Nestle, Chile HO CATHOLIC UNIV CHILE, EXPTL CTR RP Kazemi, H (reprint author), HARVARD UNIV, MASSACHUSETTS GEN HOSP, SCH MED, MED SERV, PULM & CRIT CARE UNIT, BOSTON, MA 02114 USA. NR 12 TC 2 Z9 2 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0065-2598 BN 0-306-45490-4 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1996 VL 410 BP 365 EP 369 PG 5 WC Cardiac & Cardiovascular Systems; Physiology SC Cardiovascular System & Cardiology; Physiology GA BH18J UT WOS:A1996BH18J00056 PM 9030326 ER PT J AU Fox, JG Li, XT Cahill, RJ Andrutis, K Rustgi, AK Odze, R Wang, TC AF Fox, JG Li, XT Cahill, RJ Andrutis, K Rustgi, AK Odze, R Wang, TC TI Hypertrophic gastropathy in Helicobacter felis - Infected wild-type C57BL/6 mice and p53 hemizygous transgenic mice SO GASTROENTEROLOGY LA English DT Article ID PYLORI-ASSOCIATED GASTRITIS; ACTIVE CHRONIC GASTRITIS; MENETRIERS DISEASE; NATURAL-HISTORY; CARCINOMA; CANCER; MODEL; PREVALENCE; STOMACH; TUMORS AB Background & Aims: Helicobacter pylori infection causes gastritis and peptic ulcers and is linked epidemiologically to gastric cancer, To analyze host genetic factors and the influence of Helicobacter on cell proliferation, we used an inbred and p53 hemizygous mouse model of Helicobacter felis-induced gastritis, Methods: H. felis was inoculated by gastric intubation into SPF C57BL/6 wild-type and p53 hemizygous mice that were followed up for 1 year and compared with uninfected controls of the same genotype using histology, proliferating cell nuclear antigen (PCNA) staining, and 5-bromo-2'-deoxyuridine (BrdU) analysis, Results: Infected animals developed sustained anti-H, felis serum immunoglobulin G antibody responses. Six months after infection, both wild-type and p53 hemizygous mice showed active chronic inflammation and marked mucosal hyperplasia compared with uninfected controls, One year after infection with H. felis, the wildtype and p53 hemizygous mice showed severe adenomatous and cystic hyperplasia of the surface foveolar epithelium, BrdU uptake and PCNA staining were markedly increased in both sets of infected mice compared with controls, Infected p53 hemizygous mice had a higher proliferative index than the infected wild-type mice, Conclusions: H. felis can induce a hypertrophic gastropathy in the C57BL/6 genotype; loss of one p53 allele, although insufficient to initiate carcinogenesis at 1 year, enhances the proliferative index, which may lead to an increased risk of cancer induction. C1 MIT,DIV COMPARAT MED,BOSTON,MA. MASSACHUSETTS GEN HOSP,GASTROINTESTINAL UNIT,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT MED,BOSTON,MA 02114. BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115. FU NCI NIH HHS [R01 CA67463]; NCRR NIH HHS [RR01046, RR07036] NR 39 TC 147 Z9 152 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD JAN PY 1996 VL 110 IS 1 BP 155 EP 166 DI 10.1053/gast.1996.v110.pm8536852 PG 12 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TN352 UT WOS:A1996TN35200018 PM 8536852 ER PT J AU Mithofer, K FernandezdelCastillo, C Ferraro, MJ Lewandrowski, K Rattner, DW Warshaw, AL AF Mithofer, K FernandezdelCastillo, C Ferraro, MJ Lewandrowski, K Rattner, DW Warshaw, AL TI Antibiotic treatment improves survival in experimental acute necrotizing pancreatitis SO GASTROENTEROLOGY LA English DT Article ID TRYPSINOGEN-ACTIVATION PEPTIDES; SEPTIC COMPLICATIONS; ABSCESS; CIPROFLOXACIN; PSEUDOCYST; INFECTIONS; MANAGEMENT; DIAGNOSIS; NECROSIS; IMIPENEM AB Background & Aims: It is still unproven whether prophylactic antibiotics can reduce mortality from acute necrotizing pancreatitis (ANP). The aim of this study was to investigate whether antibiotic therapy can influence long-term outcome in ANP and how appropriate this therapy is, Methods: ANP was induced in rats by standardized intraductal bile acid infusion and cerulein hyperstimulation. Serum trypsinogen activation peptide levels were used to verify comparable disease severity, Starting 6 hours after induction, animals randomly received saline (n = 60), 20 mg/kg imipenem (n = 62), or 10 mg/kg ciprofloxacin (n = 60) every 8 hours for 7 days. On day 7, half of each group was killed so a quantitative pancreatic bacteriology could be conducted. The other half was analyzed at 21 days for long-term mortality, late bacteriologic changes, abscesses, and pseudocysts. Results: Comparable trypsinogen activation peptide increases confirmed equally severe ANP in each group before treatment, Imipenem and ciprofloxacin significantly reduced the number of infected pancreatic specimens, bacterial counts, and identified species at 1 week. At 3 weeks, pancreatic infection prevalence was lower in animals treated with antibiotics; abscess formation was reduced and pseudocysts were smaller and less frequently infected, Survival was significantly improved by imipenem and ciprofloxacin. Conclusions: Antibiotic treatment reduces early and late septic pancreatic complications and improves survival from experimental ANP. C1 MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT MICROBIOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. NR 46 TC 72 Z9 73 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0016-5085 J9 GASTROENTEROLOGY JI Gastroenterology PD JAN PY 1996 VL 110 IS 1 BP 232 EP 240 DI 10.1053/gast.1996.v110.pm8536862 PG 9 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TN352 UT WOS:A1996TN35200027 PM 8536862 ER PT J AU Henry, M Borland, CZ Bossie, M Silver, PA AF Henry, M Borland, CZ Bossie, M Silver, PA TI Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3 SO GENETICS LA English DT Article ID INTACT YEAST-CELLS; MESSENGER-RNA; RIBONUCLEOPROTEIN-PARTICLES; CDNA CLONING; SPLICING FACTOR-SF2; NASCENT TRANSCRIPTS; SHUTTLE VECTORS; XENOPUS-LAEVIS; HNRNP PROTEINS; NUCLEAR AB The NPL3 gene of the yeast Saccharomyces cerevisiae encodes a protein with similarity to heterogeneous nuclear ribonucleoproteins (hnRNPs). Npl3p has been implicated in many nuclear-related events including RNA export, protein import, and rRNA processing. Several temperature-sensitive alleles of NPL3 have been isolated. We now report the sequence of these alleles. For one allele, npl3-1, four complementation groups of suppressors have been isolated. The cognate genes for the two recessive mutants were cloned. One of these is the previously known RNA15, which, like NPL3, also encodes a protein with similarity to the vertebrate hnRNP A/B protein family. The other suppressor corresponds to a newly defined gene we term HRP1, which also encodes a protein with similarity to the hnRNP A/B proteins of vertebrates. Mutations in HRP1 suppress all npl3 temperature-sensitive alleles but do not bypass an npl3 null allele. We show that HRP1 is essential for cell growth and that the corresponding protein is located in the nucleus. The discovery of two hnRNP homologues that can partially suppress the function of Npl3p, also an RNA binding protein, will be discussed in terms of the possible roles for Npl3p in RNA metabolism. C1 HARVARD UNIV, SCH MED, DEPT BIOL CHEM & MOLEC PHARMACOL, BOSTON, MA 02115 USA. DANA FARBER CANC INST, BOSTON, MA 02115 USA. NR 70 TC 81 Z9 82 U1 0 U2 1 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD JAN PY 1996 VL 142 IS 1 BP 103 EP 115 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA TM924 UT WOS:A1996TM92400011 PM 8770588 ER PT J AU Zachgo, EA Wang, ML Dewdney, J Bouchez, D Camilleri, C Belmonte, S Huang, L Dolan, M Coodman, HM AF Zachgo, EA Wang, ML Dewdney, J Bouchez, D Camilleri, C Belmonte, S Huang, L Dolan, M Coodman, HM TI A physical map of chromosome 2 of Arabidopsis thaliana SO GENOME RESEARCH LA English DT Article ID POLYMORPHISM LINKAGE MAP; DNA; SEQUENCE; GENE; IDENTIFICATION; LIBRARY; GENOME; CLONES; RFLP AB A yeast artificial chromosome (YAC) physical map of chromosome 2 of Arabidopsis thaliana has been constructed by hybridization of 69 DNA markers and 61 YAC end probes to gridded arrays of YAC clones. Thirty-four YACs in Four contigs define the chromosome. Complete closure of the map was not attained because some regions of the chromosome were repetitive or were not represented in the YAC library. Based on the sizes of the YACs and their coverage of the chromosome, the length of chromosome 2 is estimated to be at least 18 Mb. These data provide the means for immediately identifying the YACs containing a genetic locus mapped on Arabidopsis chromosome 2. C1 HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL,BOSTON,MA 02114. INRA,BIOL CELLULAIRE LAB,F-78026 VERSAILLES,FRANCE. OI Bouchez, David/0000-0003-3545-4339 NR 30 TC 78 Z9 79 U1 0 U2 0 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 SN 1054-9803 J9 GENOME RES JI Genome Res. PD JAN PY 1996 VL 6 IS 1 BP 19 EP 25 DI 10.1101/gr.6.1.19 PG 7 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA TW662 UT WOS:A1996TW66200004 PM 8681135 ER PT J AU Yoshikawa, H DelaMonte, S Nagai, H Wands, JR Matsubara, K Fujiyama, A AF Yoshikawa, H DelaMonte, S Nagai, H Wands, JR Matsubara, K Fujiyama, A TI Chromosomal assignment of human genomic NotI restriction fragments in a two-dimensional electrophoresis profile SO GENOMICS LA English DT Article ID DNA; GENES AB Using DNA from sorted human chromosomes and two-dimensional gel electrophoresis, we assigned 2295 NotI sites, 43% of the total, to specific chromosomes and designated the procedure CA-RLGS (chromosome-assigned restriction landmark genomic scanning). Although the NotI enzyme is sensitive to DNA methylation, our results suggested that the majority of the spots did not seem to be affected by this modification. The NotI sites were distributed at higher levels in chromosomes 17, 19, and 22, suggesting higher gene content in these chromosomes. Most spots were assigned to unique chromosomes, but some spots were found on two or more chromosomes. Quantitative analysis revealed the intensity of the DNA spots on the sex chromosomes to be haploid and that of the chromosome 21 spots in DNA from a male with Down syndrome to be trisomic, although there were exceptions. We report here the first-generation CA-RLGS map of the human genome. (C) 1996 Academic Press, Inc. C1 OSAKA UNIV,INST MOLEC & CELLULAR BIOL,SUITA,OSAKA 565,JAPAN. MASSACHUSETTS GEN HOSP,DIV NEUROPATHOL,BOSTON,MA 02129. HARVARD UNIV,SCH MED,BOSTON,MA 02129. MASSACHUSETTS GEN HOSP,CTR CANC,MOLEC HEPATOL LAB,BOSTON,MA 02129. NATL INST GENET,MISHIMA,SHIZUOKA 411,JAPAN. NR 23 TC 41 Z9 44 U1 0 U2 0 PU ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 SN 0888-7543 J9 GENOMICS JI Genomics PD JAN 1 PY 1996 VL 31 IS 1 BP 28 EP 35 DI 10.1006/geno.1996.0005 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA TR637 UT WOS:A1996TR63700005 PM 8808276 ER PT J AU Murayama, Y Takeda, S Yonezawa, K Giambarella, U Nishimoto, I Ogata, E AF Murayama, Y Takeda, S Yonezawa, K Giambarella, U Nishimoto, I Ogata, E TI Cell surface receptor function of amyloid precursor protein that activates Ser/Thr kinases SO GERONTOLOGY LA English DT Article; Proceedings Paper CT 7th Symposium by the 8 Winners of the Grants from Sandoz Foundation for Gerontological Research, at the Annual Meeting of the Japan-Gerontological-Society CY OCT 20, 1995 CL OSAKA, JAPAN SP Japan Gerontol Soc, Sandoz Fdn DE amyloid precursor protein; familial Alzheimer's disease; mitogen-activated protein kinase; receptor function; G protein ID NERVE GROWTH-FACTOR; ALZHEIMERS-DISEASE; MAP KINASE; SIGNAL-TRANSDUCTION; TAU-PROTEIN; MICROTUBULE; INSULIN AB Amyloid precursor protein (APP) has been shown to serve as a G(o)-coupled receptor in cell-free systems [Okamoto et al: J Biol Chem 1995;270:4205-4208], However, it has not been known whether APP exerts intracellular signaling functions in living cells. In this study, we show that stimulation of APP by anti-APP antibody as well as by a mutation found in familial Alzheimer's disease results in activation of a specific set of mitogen-activated protein kinases in multiple vertebrate cells. We conclude that APP acts as a cell surface receptor of biological relevance that turns on specific Ser/Thr kinases, and suggest that the signaling function of APP is a potential target of familial Alzheimer's disease mutations. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT MED,CARDIOVASC RES CTR,CHARLESTOWN,MA. CANC RES INST,TOKYO,JAPAN. RP Murayama, Y (reprint author), UNIV TOKYO,SCH MED,DEPT INTERNAL MED 4,BUNKYO KU,TOKYO 112,JAPAN. NR 32 TC 13 Z9 14 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0304-324X J9 GERONTOLOGY JI Gerontology PY 1996 VL 42 SU 1 BP 2 EP 11 PG 10 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA UT842 UT WOS:A1996UT84200002 PM 8804992 ER PT J AU Gollub, RL Rauch, SL AF Gollub, RL Rauch, SL TI Neuroimaging: Issues of design, resolution, and interpretation SO HARVARD REVIEW OF PSYCHIATRY LA English DT Article RP Gollub, RL (reprint author), MASSACHUSETTS GEN HOSP EAST,DEPT PSYCHIAT,CNY-2,149 13TH ST,BOSTON,MA 02129, USA. OI Gollub, Randy L./0000-0002-9434-4044 NR 7 TC 1 Z9 1 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1067-3229 J9 HARVARD REV PSYCHIAT JI Harv. Rev. Psychiatr. PD JAN-FEB PY 1996 VL 3 IS 5 BP 285 EP 289 DI 10.3109/10673229609017196 PG 5 WC Psychiatry SC Psychiatry GA TU258 UT WOS:A1996TU25800005 PM 9384958 ER PT J AU Schouten, R AF Schouten, R TI Sexual harassment and the role of psychiatry SO HARVARD REVIEW OF PSYCHIATRY LA English DT Article RP Schouten, R (reprint author), MASSACHUSETTS GEN HOSP,LAW & PSYCHIAT SERV,WARREN 605,FRUIT ST,BOSTON,MA 02114, USA. NR 10 TC 1 Z9 1 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 1067-3229 J9 HARVARD REV PSYCHIAT JI Harv. Rev. Psychiatr. PD JAN-FEB PY 1996 VL 3 IS 5 BP 296 EP 298 DI 10.3109/10673229609017199 PG 3 WC Psychiatry SC Psychiatry GA TU258 UT WOS:A1996TU25800008 PM 9384961 ER PT B AU Thornton, AF AF Thornton, AF GP AMER SOC HEAD & NECK SURG TI Updating technical advances in irradiation of head and neck neoplasia SO HEAD AND NECK CANCER, VOL 4 LA English DT Proceedings Paper CT 4th International Conference on Head and Neck Cancer CY JUL 26-AUG 02, 1996 CL TORONTO, CANADA SP Amer Soc Head & Neck Surg, Soc Head & Neck Surgeons AB Radiotherapy of head and neck tumors has undergone significant change in the past decade, paralleling advances in neurologic tumor irradiation. The advances in 1) Diagnosis and imaging 2) Treatment planning 3) Delivery of radiation 4) Combined modality therapy have all served to facilitate more aggressive radiation therapy of head and neck tumors. With each technical advance has come the opportunity to radiate with less morbidity equivalent stage disease. Alternatively, improved tumor localization and radiation delivery precision may be used to increase doses to tumors, while holding normal tissue morbidities constant. C1 Massachusetts Gen Hosp, Dept Radiat Oncol, Boston, MA 02114 USA. RP Thornton, AF (reprint author), Massachusetts Gen Hosp, Dept Radiat Oncol, Boston, MA 02114 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMERICAN SOCIETY HEAD & NECK SURGERY PI PITTSBURGH PA 203 LOTHROP ST, SUITE 519, PITTSBURGH, PA 15213 USA PY 1996 BP 216 EP 222 PG 7 WC Oncology; Otorhinolaryngology; Surgery SC Oncology; Otorhinolaryngology; Surgery GA BL47N UT WOS:000075655700031 ER PT B AU Sult, HD Spiro, IJ Fabian, R Wang, CC AF Sult, HD Spiro, IJ Fabian, R Wang, CC GP AMER SOC HEAD & NECK SURG TI Head and neck soft tissue sarcoma: Treatment by radiation alone or combined with surgery SO HEAD AND NECK CANCER, VOL 4 LA English DT Proceedings Paper CT 4th International Conference on Head and Neck Cancer CY JUL 26-AUG 02, 1996 CL TORONTO, CANADA SP Amer Soc Head & Neck Surg, Soc Head & Neck Surgeons C1 Massachusetts Gen Hosp, Dept Radiat Oncol, Boston, MA 02114 USA. RP Sult, HD (reprint author), Massachusetts Gen Hosp, Dept Radiat Oncol, Boston, MA 02114 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMERICAN SOCIETY HEAD & NECK SURGERY PI PITTSBURGH PA 203 LOTHROP ST, SUITE 519, PITTSBURGH, PA 15213 USA PY 1996 BP 385 EP 390 PG 6 WC Oncology; Otorhinolaryngology; Surgery SC Oncology; Otorhinolaryngology; Surgery GA BL47N UT WOS:000075655700054 ER PT J AU Wiatrowski, WA Giles, ER Cooke, EP AF Wiatrowski, WA Giles, ER Cooke, EP TI Development of a system to evaluate and communicate radiation risk SO HEALTH PHYSICS LA English DT Note DE operational topics; breast; risk communication; radiation, medical AB Review of research protocols involving positron emission tomography studies on healthy volunteers focused attention on the radiation exposure disclosure statements contained in the informed consent form. Of particular concern was the observation that breast doses from positron emission tomography studies are greater than breast doses from other research uses of radioisotopes, as well as routine nuclear medicine and radiographic procedures. Disclosure of individual organ doses is not normally provided on informed consent forms. A worksheet was developed to aid research investigators in the determination of effective dose equivalents and organ dose equivalents from all sources of radiation to which a volunteer is exposed. Three standardized risk statements are discussed. The final selection and use of these statements are determined by worksheet calculations of effective dose equivalents and organ dose equivalents. C1 UNIV TEXAS, HLTH SCI CTR, DEPT RADIOL SCI, SAN ANTONIO, TX 78284 USA. RP Wiatrowski, WA (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR, RADIAT SAFETY OFF 11R, 7400 MERTON MINTER BLVD, SAN ANTONIO, TX 78284 USA. NR 16 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0017-9078 EI 1538-5159 J9 HEALTH PHYS JI Health Phys. PD JAN PY 1996 VL 70 IS 1 BP 111 EP 117 DI 10.1097/00004032-199601000-00017 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health; Nuclear Science & Technology; Radiology, Nuclear Medicine & Medical Imaging GA TK957 UT WOS:A1996TK95700019 PM 7499144 ER PT J AU Collins, EG WhiteWilliams, C Jalowiec, A AF Collins, EG WhiteWilliams, C Jalowiec, A TI Spouse stressors while awaiting heart transplantation SO HEART & LUNG LA English DT Article ID CORONARY-ARTERY BYPASS; CARDIAC TRANSPLANTATION; MYOCARDIAL-INFARCTION; CANDIDATES; ADJUSTMENT; LIFE AB OBJECTIVES: The objectives of this study were to identify common stressors experienced by spouses of heart transplantation (HT) candidates; to identify differences in stressors among spouses of HT candidates based on selected demographic variables; and to report preliminary psychometric data on the newly developed Spouse Transplant Stressor Scale. DESIGN: Comparative, cross-sectional survey. SAMPLE: Spouses of 85 HT candidates awaiting HT at midwestern and southeastern medical centers and a midwestern Department of Veterans Affairs hospital. MEASURES: Spouse Transplant Stresser Scale (Collins), an investigator-developed rating form and demographic data sheet. RESULTS: Spouses of HT candidates reported high levels of stress during the wait for a donor heart. Factors related directly to the transplantation experience were rated as the most stressful. Fear that the patient (partner) would die before a heart became available was the worst stressor for the spouses. Working spouses perceived more stressors related to responsibility, socioeconomics, and self. Stressors associated with the transplantation process itself were equally stressful for spouses who work and spouses who do not work. RP Collins, EG (reprint author), US DEPT VET AFFAIRS,VET AFFAIRS EDWARD HINES JR HOSP,POB 5000,HINES,IL 60141, USA. NR 32 TC 14 Z9 14 U1 0 U2 0 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 SN 0147-9563 J9 HEART LUNG JI Heart Lung PD JAN-FEB PY 1996 VL 25 IS 1 BP 4 EP 13 DI 10.1016/S0147-9563(96)80006-9 PG 10 WC Cardiac & Cardiovascular Systems; Nursing; Respiratory System SC Cardiovascular System & Cardiology; Nursing; Respiratory System GA TU897 UT WOS:A1996TU89700002 PM 8775865 ER PT J AU Baumert, TF Liang, TJ AF Baumert, TF Liang, TJ TI Precore mutants revisited SO HEPATOLOGY LA English DT Article ID HEPATITIS-B VIRUS; FULMINANT-HEPATITIS; REGION; DNA C1 HARVARD UNIV,SCH MED,BOSTON,MA. RP Baumert, TF (reprint author), MASSACHUSETTS GEN HOSP,GASTROINTESTINAL UNIT,BOSTON,MA 02114, USA. NR 13 TC 12 Z9 12 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD JAN PY 1996 VL 23 IS 1 BP 184 EP 186 DI 10.1002/hep.510230125 PG 3 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TN801 UT WOS:A1996TN80100025 PM 8550040 ER PT J AU Nishiyama, M Furusaka, A Nishimaki, E Tanaka, T AF Nishiyama, M Furusaka, A Nishimaki, E Tanaka, T TI Ornithine decarboxylase induction during liver regeneration IRS-1-deficient mice SO HEPATOLOGY LA English DT Meeting Abstract C1 JIKEI UNIV,DAISAN HOSP,SCH MED,TOKYO,JAPAN. JIKEI UNIV,SCH MED,DEPT NUTR,TOKYO,JAPAN. JIKEI UNIV,SCH MED,DEPT INTERNAL MED,TOKYO,JAPAN. MASSACHUSETTS GEN HOSP,GASTROINTESTINAL UNIT,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0270-9139 J9 HEPATOLOGY JI Hepatology PD JAN PY 1996 VL 23 IS 1 BP P193 EP P193 PG 1 WC Gastroenterology & Hepatology SC Gastroenterology & Hepatology GA TN801 UT WOS:A1996TN80100224 ER PT J AU Esclapez, M Chang, DK Houser, CR AF Esclapez, M Chang, DK Houser, CR TI Subpopulations of GABA neurons in the dentate gyrus express high levels of the alpha(1) subunit of the GABA(A) receptor SO HIPPOCAMPUS LA English DT Article DE glutamate decarboxylase; GAD65; somatostatin; hippocampus; in situ hybridization ID MESSENGER-RNAS; RAT-BRAIN; A RECEPTOR; IMMUNOHISTOCHEMICAL LOCALIZATION; HYBRIDIZATION HISTOCHEMISTRY; BENZODIAZEPINE RECEPTORS; PRE-PROSOMATOSTATIN; BETA-2/3 SUBUNITS; DECARBOXYLASE; HETEROGENEITY AB The alpha(1) subunit of the gamma-aminobutyric acid (GABA)(A) receptor is highly expressed in a subgroup of neurons in the hippocampal formation. The distribution and chemical identities of these neurons in the dentate gyrus have been studied with double-labeling in situ hybridization and immunohistochemical methods. Double labeling for the al subunit and glutamate decarboxylase 65 (GAD65) mRNAs indicated that virtually all neurons in the dentate gyrus that are heavily labeled for the alpha(1) subunit are GABA neurons. However, many GAD65 mRNA-labeled neurons in the hilus do not contain high levels of the rut subunit mRNA and protein. Studies were thus conducted to determine if the somatostatin neurons of the hilus were part of the alpha(1) subunit-labeled group. Double labeling for the alpha(1) subunit and pre-prosomatostatin mRNAs demonstrated virtually no co-localization of these mRNAs in hilar neurons. Thus, the strongly labeled alpha(1) mRNA-containing neurons and the somatostatin neurons constitute two distinct populations of hilar GABA neurons. Double labeling for the alpha(1) subunit polypeptide and its mRNA with immunohistochemical and in situ hybridization methods demonstrated directly that neurons of the dentate gyrus that express high levels of the alpha(1) subunit mRNA are the same neurons that show extensive labeling for the alpha(1) subunit along their somal and dendritic surfaces. The high levels of alpha(1) subunit expression in some populations of GABA neurons could be related to prominent disinhibitory functions of these neurons. (C) 1996 Wiley-Liss, Inc. C1 UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90095. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROBIOL,LOS ANGELES,CA 90095. W LOS ANGELES VET AFFAIRS MED CTR,WADSWORTH DIV,NEUROL SERV,LOS ANGELES,CA 90073. W LOS ANGELES VET AFFAIRS MED CTR,WADSWORTH DIV,RES SERV,LOS ANGELES,CA 90073. RI Esclapez, Monique/O-6509-2016 OI Esclapez, Monique/0000-0002-8558-1363 FU NINDS NIH HHS [NS21908, NS29231] NR 39 TC 19 Z9 19 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1050-9631 J9 HIPPOCAMPUS JI Hippocampus PY 1996 VL 6 IS 3 BP 225 EP 238 PG 14 WC Neurosciences SC Neurosciences & Neurology GA VA305 UT WOS:A1996VA30500002 PM 8841823 ER PT J AU Oberley, TD Sempf, JM Oberley, LW AF Oberley, TD Sempf, JM Oberley, LW TI Immunogold analysis of antioxidant enzymes in common renal cancers SO HISTOLOGY AND HISTOPATHOLOGY LA English DT Article DE immunogold techniques; antioxidant enzymes; kidney cancer ID GLUTATHIONE-S-TRANSFERASE; MANGANESE SUPEROXIDE-DISMUTASE; SYRIAN-HAMSTER TISSUES; IMMUNOHISTOCHEMICAL LOCALIZATION; KIDNEY DEVELOPMENT; CELLS; EXPRESSION; LUNG; RAT; IMMUNOLOCALIZATION AB Immunogold studies of normal human kidney and common human kidney cancers were performed using polyclonal antibodies to antioxidant enzymes, including antibodies to copper, zinc and manganese superoxide dismutases, catalase, glutathione peroxidase, and glutathione S-transferases and their subunits. Normal tissue adjacent to human renal tumors had the same antioxidant enzyme immunoreactive protein profiles as normal human kidney, thus establishing that the presence of tumor does not alter the levels of antioxidant enzyme immunoreactive proteins in adjacent kidney tissue. Levels of immunoreactive protein for antioxidant enzymes were determined in four common types of malignant renal cancer. In general, tumors had low levels of antioxidant enzymes; however, certain histologic types of renal tumors had high levels of immunoreactive protein for glutathione S-transferase subunits, which could affect their susceptibility to chemotherapy. Studies of transitional carcinoma of the renal pelvis were especially informative since it was possible to compare levels of antioxidant enzyme immunoreactive protein with adjacent normal transitional epithelium; the majority of antibodies resulted in lower levels of immunoreactive protein in transitional cell carcinoma than in adjacent normal transitional epithelium. Our results are discussed in relation to the response of renal tumors to therapy. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,PATHOL & LAB MED SERV,MADISON,WI 53705. UNIV WISCONSIN,SCH MED,DEPT PATHOL,MADISON,WI 53706. UNIV IOWA,COLL MED,RADIAT RES LAB,IOWA CITY,IA. FU NCI NIH HHS [CA 41267] NR 35 TC 11 Z9 11 U1 0 U2 0 PU F HERNANDEZ PI MURCIA PA PLAZA FUENSANTA 2-7 C, 30008 MURCIA, SPAIN SN 0213-3911 J9 HISTOL HISTOPATHOL JI Histol. Histopath. PD JAN PY 1996 VL 11 IS 1 BP 153 EP 160 PG 8 WC Cell Biology; Pathology SC Cell Biology; Pathology GA TT248 UT WOS:A1996TT24800018 PM 8720459 ER PT J AU Schmahmann, JD AF Schmahmann, JD TI From movement to thought: Anatomic substrates of the cerebellar contribution to cognitive processing SO HUMAN BRAIN MAPPING LA English DT Review DE cerebellum; cerebellar nuclei; cerebral cortex; association areas; paralimbic cortices; pons; thalamus; neural circuit; cognition; behavior; affect; emotion ID POSTERIOR PARIETAL CORTEX; SUPERIOR TEMPORAL SULCUS; FRONTAL EYE FIELD; POSITRON EMISSION TOMOGRAPHY; CEREBRAL BLOOD-FLOW; RHESUS-MONKEY; HORSERADISH-PEROXIDASE; CORTICAL CONNECTIONS; MACACA-MULATTA; MACAQUE MONKEY AB The cerebellar contribution to cognitive operations and emotional behavior is critically dependent upon the existence of plausible anatomic substrates. This paper explores these anatomic substrates, namely, the incorporation of the associative and paralimbic cerebral areas into the cerebrocerebellar circuitry in nonhuman primates. Using the novel information that has emerged concerning this system, proposed rules are derived and specific hypotheses offered concerning cerebellar function and the relationship between cerebellum and nonmotor behavior, as follow. (1) The associative and paralimbic incorporation into the cerebrocerebellar circuit is the anatomic underpinning of the cerebellar contribution to cognition and emotion. (2) There is topographic organization of cognitive and behavioral functions within the cerebellum. The archicerebellum, vermis, and fastigial nucleus are principally concerned with affective and autonomic regulation and emotionally relevant memory. The cerebellar hemispheres and dentate nucleus are concerned with executive, visual-spatial, language, and other mnemonic functions. (3) The convergence of inputs from multiple associative cerebral regions to common areas within the cerebellum facilitates cerebellar regulation of supramodal functions. (4) The cerebellar contribution to cognition is one of modulation rather than generation. Dysmetria of (or ataxic) thought and emotion are the clinical manifestations of a cerebellar lesion in the cognitive domain. (5) The cerebellum performs the same computations for associative and paralimbic functions as it does for the sensorimotor system. These proposed rules and the general and specific hypotheses offered in this paper are testable using functional neuroimaging techniques. Neuroanatomy and functional neuroimaging may thus be mutually advantageous in predicting and explaining new concepts of cerebellar function. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. RP Schmahmann, JD (reprint author), MASSACHUSETTS GEN HOSP,DEPT NEUROL,BURNHAM 823,FRUIT ST,BOSTON,MA 02114, USA. NR 200 TC 297 Z9 301 U1 3 U2 13 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1065-9471 J9 HUM BRAIN MAPP JI Hum. Brain Mapp. PY 1996 VL 4 IS 3 BP 174 EP 198 DI 10.1002/(SICI)1097-0193(1996)4:3<174::AID-HBM3>3.3.CO;2-W PG 25 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA VZ944 UT WOS:A1996VZ94400003 PM 20408197 ER PT J AU Carrington, M Marti, D Malasky, M Barcellos, L Wade, J Awdeh, Z Truedsson, L AF Carrington, M Marti, D Malasky, M Barcellos, L Wade, J Awdeh, Z Truedsson, L TI Mapping disease loci within the MHC by typing microsatellite loci. SO HUMAN IMMUNOLOGY LA English DT Meeting Abstract C1 NCI,SAIC,FCRDC,FREDERICK,MD 21701. UNIV CALIF BERKELEY,SCH PUBL HLTH,BERKELEY,CA 94720. UNIV TORONTO,TTH REG HISTO LAB,TORONTO,ON M5S 1A1,CANADA. CTR BLOOD RES,BOSTON,MA 02115. LUND UNIV,DEPT MED MICROBIOL,S-22100 LUND,SWEDEN. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PY 1996 VL 49 SU 1 BP 24 EP 24 DI 10.1016/S0198-8859(96)89951-4 PG 1 WC Immunology SC Immunology GA VD912 UT WOS:A1996VD91200043 ER PT J AU Fitzpatrick, D Drew, L Saidman, SL AF Fitzpatrick, D Drew, L Saidman, SL TI A simple method for removal of OCT3 from patient sera. SO HUMAN IMMUNOLOGY LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,HISTOCOMPATIBIL LAB,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PY 1996 VL 49 SU 1 BP 131 EP 131 PG 1 WC Immunology SC Immunology GA VD912 UT WOS:A1996VD91200209 ER PT J AU Perlee, L Freeman, B Calorossi, M Umland, K Deulofeut, R Balazs, I AF Perlee, L Freeman, B Calorossi, M Umland, K Deulofeut, R Balazs, I TI Identification of two new class II DR beta alleles by solid phase sequencing of PCR products. SO HUMAN IMMUNOLOGY LA English DT Meeting Abstract C1 LIFECODES CORP,STAMFORD,CT 06902. CTR BLOOD RES,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PY 1996 VL 49 SU 1 BP 144 EP 144 PG 1 WC Immunology SC Immunology GA VD912 UT WOS:A1996VD91200222 ER PT J AU Yunis, JJ Yunis, EJ Deulofeut, R Salazar, M Ossa, H Yunis, E AF Yunis, JJ Yunis, EJ Deulofeut, R Salazar, M Ossa, H Yunis, E TI Unusual high frequencies for class II allele and haplotypes of the Guambiano, Paez and Ingano (Inga) tribes of Colombia may indicate a common ancestral origin. SO HUMAN IMMUNOLOGY LA English DT Meeting Abstract C1 UNIV NACL COLOMBIA,INST GENET,BOGOTA,COLOMBIA. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115. AMER RED CROSS,BLOOD SERV,DEDHAM,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0198-8859 J9 HUM IMMUNOL JI Hum. Immunol. PY 1996 VL 49 SU 1 BP 195 EP 195 PG 1 WC Immunology SC Immunology GA VD912 UT WOS:A1996VD91200273 ER PT J AU Gulley, ML Pulitzer, DR Eagan, PA Schneider, BG AF Gulley, ML Pulitzer, DR Eagan, PA Schneider, BG TI Epstein-Barr virus infection is an early event in gastric carcinogenesis and is independent of bcl-2 expression and p53 accumulation SO HUMAN PATHOLOGY LA English DT Article DE gastric carcinoma; Epstein-Barr virus; p53; bcl-2; Hispanic ID LYMPHOEPITHELIOMA-LIKE CARCINOMA; POLYMERASE CHAIN-REACTION; LYMPHOMA CELL-LINES; NASOPHARYNGEAL CARCINOMA; INSITU HYBRIDIZATION; PROTOONCOGENE EXPRESSION; CANCER; ADENOCARCINOMA; STOMACH; ASSOCIATION AB Ninety-five cases of adenocarcinoma of the stomach were evaluated for the presence of Epstein-Barr virus (EBV) using a sensitive in situ hybridization assay targeting Epstein-Barr virus-encoded RNA 1 (EBER1) transcripts. EBER1 was detected in 11 of 95 (12%) of cases. When present, the virus was localized to malignant epithelial cells and to dysplastic gastric epithelium, but was not seen in normal-appearing gastric epithelium or intestinal metaplasia. The EBV DNA was monoclonal in all three cases tested by Southern blot analysis of the EBV terminal repeat fragment. These findings suggest that the virus was present before malignant transformation. The presence of EBV was strongly associated with increased numbers of tumorinfiltrating T lymphocytes; however, EBV was not associated with prolonged survival. Neither p53 nor bcl-2 were consistently detected in the EBV-associated tumors. Specifically, 6 of Ii EBV-positive carcinomas had accumulation of p53 protein by immunohistochemical analysis, which was similar to the prevalence of p53 accumulation in EBV-negative specimens and suggests that EBV infection does not substitute for p53 mutation during tumorigenesis. The bcl-2 oncoprotein was expressed in a third of the carcinoma specimens tested, but bcl-2 expression did not correlate with the presence of EBV or with expression of EBV latent membrane protein 1. In conclusion, EBV infection appears to precede malignant transformation in a significant fraction of gastric carcinomas, but neither bcl-2 expression nor p53 accumulation appear to be consistently associated with the presence of the virus. C1 AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Gulley, ML (reprint author), UNIV TEXAS,HLTH SCI CTR,DEPT PATHOL,7703 FLOYD CURL DR,SAN ANTONIO,TX 78284, USA. FU NCI NIH HHS [K08-CA01615, P30-CA54174] NR 62 TC 108 Z9 114 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0046-8177 J9 HUM PATHOL JI Hum. Pathol. PD JAN PY 1996 VL 27 IS 1 BP 20 EP 27 DI 10.1016/S0046-8177(96)90133-1 PG 8 WC Pathology SC Pathology GA TP794 UT WOS:A1996TP79400005 PM 8543306 ER PT J AU Yang, WI Efird, JT QuintanillaMartinez, L Choi, N Harris, NL AF Yang, WI Efird, JT QuintanillaMartinez, L Choi, N Harris, NL TI Cell kinetic study of thymic epithelial tumors using PCNA (PC10) and Ki-67 (MIB-1) antibodies SO HUMAN PATHOLOGY LA English DT Article DE thymic epithelial tumors; thymoma; Ki-67 (MIB-1); PCNA (PC10); proliferation index; immunohistochemistry ID DNA FLOW-CYTOMETRY; NUCLEAR ANTIGEN; MONOCLONAL-ANTIBODIES; PATHOLOGICAL CLASSIFICATION; THYMOMA; CARCINOMA; PROLIFERATION; GRADE AB We performed an immunohistochemical cell kinetic study with monoclonal antibodies to proliferating cell nuclear antigen (PCNA)- PC10- and Ki-67 - MIB1 - on 62 thymic epithelial tumors, to evaluate whether there is correlation between the proliferation indices of the neoplastic epithelial cells and histological subtype, stage, and risk of relapse. The 62 cases of thymic epithelial tumors were classified as medullary thymoma (4 cases), composite (mixed) thymoma (17 cases), organoid thymoma (predominantly cortical) (11 cases), cortical thymoma (10 cases), well-differentiated thymic carcinoma (18 cases), and poorly differentiated thymic carcinoma (2 cases). Labeling indices were expressed as percentage of epithelial cells with positive nuclear immunostaining by random counting of 1,000 epithelial tumor cells, using an oil immersion 100X objective. PCNA labeling indices were consistently higher than those of Ki-67, and they correlated with each other. Well-differentiated thymic carcinoma showed higher labeling indices (3.11% +/- 3.53%) by Ki-67 antibody compared with the medullary type (0.60% +/- 0.07%) (P<.05) but there were no statistically significant differences between the other histological subtypes. Stage IV cases showed higher PCNA labeling indices (PCNA: 11.07% +/- 7.35%, Ki-67: 6.86% +/- 5.87%) than cases of the other stages (P<.05), but there were no statistically significant differences in either labeling index between the other stages. The number of patients who relapsed was too small to permit meaningful correlation between labeling indices and relapse. Our results indicate that the differences in biological behavior of the different histological subtypes of thymic epithelial tumors may be in part explained by differences in tumor growth fraction. Analysis of a larger group of patients will be required to determine whether proliferation fraction as determined by this method can be used to predict outcome in individual cases. HUM PATHOL 27:70-76. Copyright (C) 1996 by W.B. Saunders Company C1 MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT RADIAT ONCOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. YONSEI UNIV,COLL MED,DEPT PATHOL,SEOUL 120749,SOUTH KOREA. INST NACL NUTR,DEPT PATHOL,TLALPAN,MEXICO. NR 31 TC 18 Z9 23 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0046-8177 J9 HUM PATHOL JI Hum. Pathol. PD JAN PY 1996 VL 27 IS 1 BP 70 EP 76 DI 10.1016/S0046-8177(96)90140-9 PG 7 WC Pathology SC Pathology GA TP794 UT WOS:A1996TP79400012 PM 8543314 ER PT J AU Younis, AI Toner, M Albertini, DF Biggers, JD AF Younis, AI Toner, M Albertini, DF Biggers, JD TI Cryobiology of non-human primate oocytes SO HUMAN REPRODUCTION LA English DT Article DE cryomicroscopy; cynomolgus oocytes; freezing; morphology; rhesus ID INTRACELLULAR ICE FORMATION; MOUSE OOCYTES; BOVINE OOCYTES; TEMPERATURE-DEPENDENCE; INVITRO FERTILIZATION; WATER PERMEABILITY; ACTIVATION-ENERGY; EMBRYOS; CRYOPRESERVATION; CYTOSKELETON AB The responses to various stresses involved with cryopreservation protocols were investigated using non-human primate oocytes, Fluorescence microscopy was used to assess the status of the F-actin microfilament system of rhesus monkey oocytes after exposure to different concentrations of glycerol, The F-actin organization around the cortex and in the transzonal processes was modified by exposure to 1.0 or 2.0 M glycerol at ambient temperature, These effects were reduced significantly when exposure to glycerol was combined with cooling to 0 degrees C, Cynomolgus monkey oocytes were also subjected to hyperosmotic stress and observed for morphological changes. An irregular shrinkage phenomenon was observed with germinal vesicle or metaphase I but not metaphase II (MII) oocytes, The irregular shrinkage became uniform and spherical when the oocytes were pretreated with ethyleneglycol-bis-(beta-aminoethyl ether)N,N,N',N' tetraacetic acid (EGTA) before exposure to hypertonic solution, Also, in-vitro-matured MII oocytes from cynomolgus monkeys were used to determine crucial biophysical parameters for freezing primate oocytes, The permeability of oocyte plasma membrane to water, L(pg), and its activation energy, E(Lp), were determined between 0 and -12 degrees C in the absence of cryoprotective additives, The L(pg) was found to be 3.8X10(-14) m(3)N/s and the E(Lp) was 141.5 kJ/mol, The pre-exponential kinetic and exponential thermodynamic parameters of intracellular ice formation were determined to be 8 x 10(8) m(2)/s and 2.2 x 10(9) K-5 respectively, By combining models of water transport and intracellular ice formation, the cumulative fraction of oocytes with intracellular ice as a function of the cooling rate was also predicted, and it was shown to correlate reasonably with experimental observations. C1 HARVARD UNIV,SCH MED,DEPT CELL BIOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT SURG,BOSTON,MA 02129. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SURG RES LAB,BOSTON,MA 02129. TUFTS UNIV,HLTH SCI CTR,DEPT ANAT & CELLULAR BIOL,BOSTON,MA 02111. HARVARD UNIV,SCH MED,DIV REPROD BIOL,NEW ENGLAND REG PRIMATE RES CTR,SOUTHBOROUGH,MA 01772. FU NICHD NIH HHS [UO1 HD 21988]; OCPHP CDC HHS [R24 PR02510, P51PR00168] NR 39 TC 49 Z9 51 U1 0 U2 0 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0268-1161 J9 HUM REPROD JI Hum. Reprod. PD JAN PY 1996 VL 11 IS 1 BP 156 EP 165 PG 10 WC Obstetrics & Gynecology; Reproductive Biology SC Obstetrics & Gynecology; Reproductive Biology GA TV681 UT WOS:A1996TV68100035 PM 8671179 ER PT B AU Gow, DW Melvold, J Manuel, S AF Gow, DW Melvold, J Manuel, S BE Bunnell, HT Idsardi, W TI How word onsets drive lexical access and segmentation: Evidence from acoustics, phonology and processing SO ICSLP 96 - FOURTH INTERNATIONAL CONFERENCE ON SPOKEN LANGUAGE PROCESSING, PROCEEDINGS, VOLS 1-4 LA English DT Proceedings Paper CT 4th International Congress on Spoken Language Processing CY OCT 03-06, 1996 CL PHILADELPHIA, PA SP Univ Delaware, Alfred I DuPont Inst, Acoust Soc Amer, Acoust Soc Japan, Amer Speech Language Hearing Assoc, Austr Speech Sci & Technol Assoc, European Speech Commun Assoc, IEEE Signal Proc Soc, Inc Canadian Acoust Assoc, Int Phonet Assoc, Linguist Soc Amer AB We will argue that the beginnings of words are perceptual ''islands of reliability'' in connected speech, and that their perceptual and temporal properties allow them to drive critical aspects of spoken word recognition including lexical segmentation. This argument rests on three generalizations derived from research in speech science, phonology, and psycholinguistics. We suggest that word onsets differ from other parts of words in that: (1) they offer more robust and redundant acoustic evidence about phonetic features, (2) they are generally protected from phonological assimilation, neutralization and deletion and therefore show less lawful variation in surface realization, and (3) they may activate lexical representations which facilitate word perception and thus diminish listeners' dependence on veridical acoustic-phonetic processing of other portions of words. These properties of word onsets allow them to drive lexical segmentation by facilitating the recognition of items that begin with clear onsets. The implications of these findings for several models of lexical segmentation and spoken word recognition are discussed. RP Gow, DW (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CAMBRIDGE,MA 02138, USA. NR 0 TC 11 Z9 11 U1 0 U2 1 PU I E E E PI NEW YORK PA 345 E 47TH ST, NEW YORK, NY 10017 BN 0-7803-3555-4 PY 1996 BP 66 EP 69 PG 4 WC Acoustics; Engineering, Electrical & Electronic; Language & Linguistics; Psychology, Experimental SC Acoustics; Engineering; Linguistics; Psychology GA BJ20B UT WOS:A1996BJ20B00018 ER PT J AU Gonzalo, JA Jia, GQ Aguirre, V Friend, D Coyle, AJ Jenkins, NA Lin, GS Katz, H Lichtman, A Copeland, N Kopf, M GutierrezRamos, JC AF Gonzalo, JA Jia, GQ Aguirre, V Friend, D Coyle, AJ Jenkins, NA Lin, GS Katz, H Lichtman, A Copeland, N Kopf, M GutierrezRamos, JC TI Mouse eotaxin expression parallels eosinophil accumulation during lung allergic inflammation but it is not restricted to a Th2-type response SO IMMUNITY LA English DT Article ID COLONY-STIMULATING FACTOR; CELL-ADHESION MOLECULE-1; TUMOR-NECROSIS-FACTOR; GROWTH-FACTOR; DIFFERENTIATION FACTOR; GENE JE; CYTOKINE; IL-4; CLONING; INTERLEUKIN-5 AB A model of lung eosinophilia based on the repeated exposure of mice to aerosolized OVA has been used to identify C-C chemokine genes expressed at stages of massive eosinophil infiltration. We describe the identification and cloning of a cDNA that encodes a mouse C-C chemokine with 68% amino acid identity to guinea pig Eotaxin. The recombinant protein encoded by this gene displays potent and specific chemotactic activity for eosinophils, both in vivo and in vitro. Its mRNA levels parallel the kinetics of eosinophil accumulation in the lung during the experimentally induced eosinophilia and it is mainly produced by type I alveolar epithelial cells. The mRNA expression of mouse Eotaxin is not restricted to Th2 T cells in vitro and is independent of the development of a Th2-type response during N. brasiliensis infection, in vivo. C1 HARVARD UNIV,SCH MED,DEPT GENET,CAMBRIDGE,MA 02138. BRIGHAM & WOMENS HOSP,DIV RHEUMATOL,BOSTON,MA 02115. NCI,FREDERICK CANC RES & DEV CTR,MAMMALIAN GENET LAB,FREDERICK,MD 21702. BASEL INST IMMUNOL,BASEL,SWITZERLAND. RP Gonzalo, JA (reprint author), HARVARD UNIV,SCH MED,CTR BLOOD RES INC,CAMBRIDGE,MA 02138, USA. NR 62 TC 154 Z9 156 U1 0 U2 1 PU CELL PRESS PI CAMBRIDGE PA 1050 MASSACHUSETTES AVE, CIRCULATION DEPT, CAMBRIDGE, MA 02138 SN 1074-7613 J9 IMMUNITY JI Immunity PD JAN PY 1996 VL 4 IS 1 BP 1 EP 14 DI 10.1016/S1074-7613(00)80293-9 PG 14 WC Immunology SC Immunology GA TT319 UT WOS:A1996TT31900001 ER PT J AU Cai, WY Cao, W Wu, LZ Exley, GE Waneck, GL Karger, BL Warner, CM AF Cai, WY Cao, W Wu, LZ Exley, GE Waneck, GL Karger, BL Warner, CM TI Sequence and transcription of Qa-2-encoding genes in mouse lymphocytes and blastocysts SO IMMUNOGENETICS LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-I GENE; DEVELOPMENT PED GENE; QA-2 ANTIGEN; Q-REGION; EXPRESSION; MHC; MOLECULES; PHENOTYPE; EMBRYOS AB The protein product of the mouse preimplantation embryo development (Fed) gene, which controls the rate of preimplantation embryonic cleavage division and subsequent embryo survival, is the Qa-2 antigen. This major histocompatibility complex (MHC) class Ib protein is encoded by four genes, Q6, Q7, Q8, and Q9. The present study was undertaken to begin to elucidate which of the four Qa-2-encoding genes are responsible for the Fed gene phenotype in the C57BL/6 mouse (H2b). First, restriction maps of the four genes, using 25 restriction enzymes, were created. The RE maps confirmed that Q6 is similar to Q8 and Q7 is similar to Q9, but that the Q6/Q8 gene pair differs from the Q7/Q9 gene pair. The genomic DNA sequences of Q6 and Q8 were determined, as well as the DNA sequences of exons 4-8 of Q9, and the 5' regulatory regions of Q6, Q8, and Q9. This DNA sequence information, combined with the published DNA sequence information for the entire Q7 gene and exons 1-3 of Q9, allowed us to design primers for reverse transcription-polymerase chain reaction that could distinguish which of the four genes were transcribed in mouse lymphocytes and embryos. It was found that all four genes are transcribed in lymphocytes, but only Q7 and Q9 are transcribed in mouse embryos. Thus, both Q7 and Q9 are candidates for the genes responsible for the Ped gene phenotype. C1 NORTHEASTERN UNIV,DEPT BIOL,BOSTON,MA 02115. MASSACHUSETTS GEN HOSP,DEPT SURG,CHARLESTOWN,MA 02129. NORTHEASTERN UNIV,DEPT CHEM,BOSTON,MA 02115. NORTHEASTERN UNIV,BARNETT INST CHEM ANAL & MAT SCI,BOSTON,MA 02115. FU NICHD NIH HHS [HD31505]; NIGMS NIH HHS [GM46467] NR 29 TC 27 Z9 27 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0093-7711 J9 IMMUNOGENETICS JI Immunogenetics PY 1996 VL 45 IS 2 BP 97 EP 107 DI 10.1007/s002510050177 PG 11 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA VZ156 UT WOS:A1996VZ15600002 PM 8952959 ER PT J AU Tan, KN Huang, K Myrick, KV Tanigawa, G AF Tan, KN Huang, K Myrick, KV Tanigawa, G TI Diversity of the Tcra-V3 gene family in BALB/c mice SO IMMUNOGENETICS LA English DT Article ID CELL ANTIGEN RECEPTOR; ALPHA-FAMILY; V-ALPHA; MOUSE; ORGANIZATION; SEGMENTS; FRAGMENTS AB Southern analysis of EcoRI-digested BALB/c Liver DNA reveals four T-cell receptor Tcra-V3-hybridizing DNA fragments, which are of sizes 18.0, 12.0, 8.0, and 2.1 kilobases, respectively. These four Tcra-V3-hybridizing genomic DNA were isolated from a BALB/c genomic library. Restriction and Southern analysis of the genomic DNA clones showed that each of the Tcra-V3-hybridizing Eco RI DNA fragments harbors only a single Tcra-V3 gene. The DNA sequences of coding regions of the four Tcra-V3 family members were determined. These sequences show very limited divergence from one another. Comparisons of BALB/c Tcra-V3 sequences with published Tcra-V3 sequences expressed in different strains of mice reveal substantial allelic polymorphism. Sequence similarity searches retrieved homologous rat, cattle, and human genes. The scarcity of coding sequence divergence among members of the Tcra-V3 family and the more substantial allelic polymorphism may be general features of the T-cell receptor V-alpha chain-encoding gene families. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,IMMUNOPATHOL LAB,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. MEM SLOAN KETTERING CANC CTR,CELLULAR BIOCHEM & BIOPHYS PROGRAM,NEW YORK,NY 10021. FU NIDDK NIH HHS [R01 DK46382] NR 15 TC 3 Z9 3 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0093-7711 J9 IMMUNOGENETICS JI Immunogenetics PY 1996 VL 44 IS 5 BP 372 EP 376 PG 5 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA VG285 UT WOS:A1996VG28500007 PM 8781123 ER PT J AU Krenger, W Ferrara, JLM AF Krenger, W Ferrara, JLM TI Graft-versus-host disease and the Th1/Th2 paradigm SO IMMUNOLOGIC RESEARCH LA English DT Review DE graft-versus-host disease; T cell cytokines; bone marrow; transplantation; Th1/Th2 dichotomy ID TUMOR-NECROSIS-FACTOR; BONE-MARROW TRANSPLANTATION; NITRIC-OXIDE PRODUCTION; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; MINOR HISTOCOMPATIBILITY ANTIGENS; RECEPTOR MONOCLONAL-ANTIBODY; MOUSE RADIATION CHIMERAS; HUMAN-ENDOTHELIAL CELLS; GROWTH-FACTOR-BETA; T-HELPER CELL AB Graft-versus-host disease (GVHD) is the major complication after allogeneic bone marrow transplantation (BRIT) and is initiated by alloreactive donor T cells recognizing foreign histocompatibility antigens of the host, There is now substantial experimental and clinical evidence to implicate a dysregulation of cytokine networks as a primary cause for the induction and maintenance of GVHD, In this article, current knowledge of the involvement of cytokines in GVHD is reviewed, The balance between type 1 cytokines (interleukin-2, interferon-gamma) and type 2 cytokines (interleukin-4, interleukin-10) is hypothesized to govern the extent to which a cell-mediated immune response and a systemic inflammatory response develop after allogeneic BRIT, Because type 2 cytokines can inhibit the production of the proinflammatory cytokines interleukin-1 and tumor necrosis factor-alpha, a type 1 to type 2 shift in the initial response of donor T cells to host alloantigens may interrupt the cytokine cascade after allogeneic BRIT and may offer a new approach to the prevention and treatment of acute GVHD, Interventions to specifically eliminate or modify the response of donor T cells to alloantigens in order to reduce GVHD may obviate the need for T cell depletion in clinical BRIT and thus avoid the increased risk of relapse of malignancy and impairment of donor cell engraftment. C1 HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. RP Krenger, W (reprint author), CHILDRENS HOSP,DANA FARBER CANC INST,DIV PEDIAT ONCOL D1638,44 BINNEY ST,BOSTON,MA 02115, USA. FU NCI NIH HHS [CA 39542]; NIAID NIH HHS [AI 30018] NR 174 TC 111 Z9 117 U1 0 U2 1 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 0257-277X J9 IMMUNOL RES JI Immunol. Res. PY 1996 VL 15 IS 1 BP 50 EP 73 DI 10.1007/BF02918284 PG 24 WC Immunology SC Immunology GA UJ790 UT WOS:A1996UJ79000005 PM 8739565 ER PT J AU Janssen, O LenglJanssen, B Oberg, HH Robertson, MJ Kabelitz, D AF Janssen, O LenglJanssen, B Oberg, HH Robertson, MJ Kabelitz, D TI Induction of cell death via Fas (CD95, Apo-1) may be associated with but is not dependent on Fas-induced tyrosine phosphorylation SO IMMUNOLOGY LETTERS LA English DT Article DE CD95; Fas antigen; apoptosis; signaling; tyrosine phosphorylation; T cells ID TUMOR-NECROSIS-FACTOR; FACTOR-ALPHA; CERAMIDE; APOPTOSIS; SPHINGOMYELINASE; DIFFERENTIATION; RECEPTOR; PATHWAY AB Cross-linking of the Fas-antigen (CD95, Apo-1) triggers apoptosis in activated T cells and transformed T cell lines. Fas-induced apoptosis has been previously reported to require Fas-triggered tyrosine phosphorylation of various proteins. In the present study, we have compared the protein tyrosine phosphorylation pattern and the apoptosis sensitivity in a set of Jurkat variants selected for the absence or presence of T cell receptor (TCR)/CD3 expression and resistance or sensitivity to Fas-mediated apoptosis. While tyrosine phosphorylation upon Fas-ligation was readily apparent in wild-type Jurkat cells (which are sensitive to anti-Fas-induced apoptosis), drastically reduced tyrosine phosphorylation was observed in Fas-resistant Jurkat subclones (which still express CD95 on their surface). More importantly, TCR/CD3-negative Jurkat variants which expressed normal levels of CD95 and were fully susceptible to Fas-triggered cell death, did not show any protein tyrosine phosphorylation upon Fas-ligation. Taken together, our data demonstrate that Fas-induced cell death can be associated with but is not dependent on protein tyrosine phosphorylation. C1 DANA FARBER CANC INST,DIV HEMATOL MALIGNANCIES,BOSTON,MA 02115. RP Janssen, O (reprint author), PAUL EHRLICH INST,DEPT IMMUNOL,PAUL EHRLICH STR 51-59,D-63225 LANGEN,GERMANY. RI Kabelitz, Dieter/A-2757-2010; Janssen, Ottmar/E-9735-2010 NR 35 TC 22 Z9 22 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2478 J9 IMMUNOL LETT JI Immunol. Lett. PD JAN PY 1996 VL 49 IS 1-2 BP 63 EP 69 DI 10.1016/0165-2478(95)02482-4 PG 7 WC Immunology SC Immunology GA UC578 UT WOS:A1996UC57800011 PM 8964611 ER PT S AU Garrido, L Bogdanova, A Cheng, LL Pfleiderer, B Tokareva, E Ackerman, JL Brady, TJ AF Garrido, L Bogdanova, A Cheng, LL Pfleiderer, B Tokareva, E Ackerman, JL Brady, TJ BE Potter, M Rose, NR TI Detection of silicone migration and biodegradation with NMR SO IMMUNOLOGY OF SILICONES SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Article; Proceedings Paper CT Workshop on the Immunology of Silicones CY MAR 13-14, 1995 CL NIH, NATCHER CONF CTR, BETHESDA, MD HO NIH, NATCHER CONF CTR ID BREAST IMPLANTS; AUGMENTATION MAMMAPLASTY; SYSTEMIC-SCLEROSIS; MAMMOPLASTY; DEGRADATION; PROSTHESES; DISEASES; WOMEN C1 HARVARD UNIV,SCH MED,CHARLESTOWN,MA. RP Garrido, L (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIOL,NMR CTR,CHARLESTOWN,MA, USA. RI Garrido, Leoncio/K-3092-2014; Ackerman, Jerome/E-2646-2015 OI Garrido, Leoncio/0000-0002-7587-1260; Ackerman, Jerome/0000-0001-5176-7496 NR 34 TC 9 Z9 9 U1 1 U2 2 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X BN 3-540-60272-0 J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 210 BP 49 EP 58 PG 10 WC Immunology; Microbiology SC Immunology; Microbiology GA BF83W UT WOS:A1996BF83W00005 PM 8565588 ER PT S AU Bridges, AJ Anderson, JD Burns, DE Kemple, K Kaplan, JD Lorden, T AF Bridges, AJ Anderson, JD Burns, DE Kemple, K Kaplan, JD Lorden, T BE Potter, M Rose, NR TI Autoantibodies in patients with silicone implants SO IMMUNOLOGY OF SILICONES SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Article; Proceedings Paper CT Workshop on the Immunology of Silicones CY MAR 13-14, 1995 CL NIH, NATCHER CONF CTR, BETHESDA, MD HO NIH, NATCHER CONF CTR ID CONNECTIVE-TISSUE DISEASE; ANTINUCLEAR ANTIBODIES; FIBROMYALGIA SYNDROME; BREAST IMPLANTS; FEATURES; SYMPTOMS; WOMEN AB We assessed the prevalence of autoantibodies in women with silicone implants and controls. Five hundred consecutive patients with silicone implants, 25 age-matched normal women, 25 women with silicone implants and no rheumatic symptoms, and 100 women with fibromyalgia were tested. Immunofluorescence antinuclear antibodies (ANA) were performed using HEp-2 cells. Subtype autoantibodies were performed by enzyme-linked immunoassay and Western blot. ANA tests were positive in 30% of patients with silicone implants and rheumatic symptoms, 8% of age-matched normal women, 28% of women with silicone implants without clinical symptoms, and 25% of women with fibromyalgia and no silicone implants. The predominant ANA pattern was speckled (55%). ANA subtype testing was positive in 4.8% of patients and none of the controls. We conclude that a larger proportion of women with silicone implants have autoantibodies compared to age-matched asymptomatic women suggesting immune activation in women with silicone implants. RP Bridges, AJ (reprint author), UNIV WISCONSIN,HOSP & CLIN,WILLIAM S MIDDLETON MEM VET HOSP,SECT RHEUMATOL,DEPT MED,MADISON,WI 53705, USA. NR 11 TC 7 Z9 8 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X BN 3-540-60272-0 J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 210 BP 277 EP 282 PG 6 WC Immunology; Microbiology SC Immunology; Microbiology GA BF83W UT WOS:A1996BF83W00028 PM 8565567 ER PT S AU Silverman, S Gluck, O Silver, D Tesser, J Wallace, D Neumann, K Metzger, A Morris, R AF Silverman, S Gluck, O Silver, D Tesser, J Wallace, D Neumann, K Metzger, A Morris, R BE Potter, M Rose, NR TI The prevalence of autoantibodies in symptomatic and asymptomatic patients with breast implants and patients with fibromyalgia SO IMMUNOLOGY OF SILICONES SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Article; Proceedings Paper CT Workshop on the Immunology of Silicones CY MAR 13-14, 1995 CL NIH, NATCHER CONF CTR, BETHESDA, MD HO NIH, NATCHER CONF CTR ID CLASSIFICATION; ANTIBODIES; CRITERIA; DISEASE; WOMEN C1 UNIV CALIF LOS ANGELES,SCH MED,LOS ANGELES,CA. CEDARS SINAI MED CTR,LOS ANGELES,CA 90048. ARIZONA RHEUMATOL CTR LTD,PHOENIX,AZ. RHEUMATOL DIAGNOST LABS INC,LOS ANGELES,CA. RP Silverman, S (reprint author), W LOS ANGELES VA,LOS ANGELES,CA, USA. NR 13 TC 3 Z9 3 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X BN 3-540-60272-0 J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 210 BP 317 EP 322 PG 6 WC Immunology; Microbiology SC Immunology; Microbiology GA BF83W UT WOS:A1996BF83W00033 PM 8565573 ER PT S AU Gollob, JA Ritz, J AF Gollob, JA Ritz, J BE Lotze, MT Trinchieri, G Gately, M Wolf, S TI CD2-CD58 interaction and the control of T-cell interleukin-12 responsiveness - Adhesion molecules link innate and acquired immunity SO INERLEUKIN 12: CELLULAR AND MOLECULAR IMMUNOLOGY OF AN IMPORTANT REGULATORY CYTOKINE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Interleukin-12 - Cellular and Molecular Immunology of an Important Regulatory Cytokine CY NOV 09-12, 1995 CL NEW YORK, NEW YORK SP New York Acad Sci, Genet Inst Inc, Hoffmann La Roche Inc, Boehringer Ingelheim Pharm Inc, Bristol Myers Squibb Pharm Res Inst, Lab Line Instruments Inc, Merck & Co Inc, NCI, Pfizer Inc, Upjohn Co, SmithKline Beecham Pharm, Wyeth Ayerst Res ID STIMULATORY FACTOR; LYMPHOCYTE-T; TYROSINE PHOSPHORYLATION; INTERFERON-GAMMA; TH1 CELLS; HUMAN CD2; NK CELLS; IL-12; ACTIVATION; PROLIFERATION C1 Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Med,Div Hematol Malignancies, Boston, MA 02115 USA. RP Gollob, JA (reprint author), Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Med,Div Hematol Malignancies, Mayer Bldg 726,44 Binney St, Boston, MA 02115 USA. OI Ritz, Jerome/0000-0001-5526-4669 NR 25 TC 18 Z9 22 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-018-2 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 795 BP 71 EP 81 DI 10.1111/j.1749-6632.1996.tb52656.x PG 11 WC Biochemistry & Molecular Biology; Cell Biology; Immunology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Immunology; Science & Technology - Other Topics GA BK04A UT WOS:000070968300008 PM 8958918 ER PT S AU Rook, AH Kubin, M Foz, FE Niu, ZT Cassin, M Vowels, BR Gottleib, SL Vonderheid, EC Lessin, SR Trinchieri, G AF Rook, AH Kubin, M Foz, FE Niu, ZT Cassin, M Vowels, BR Gottleib, SL Vonderheid, EC Lessin, SR Trinchieri, G BE Lotze, MT Trinchieri, G Gately, M Wolf, S TI The potential therapeutic role of interleukin-12 in cutaneous T-cell lymphoma SO INERLEUKIN 12: CELLULAR AND MOLECULAR IMMUNOLOGY OF AN IMPORTANT REGULATORY CYTOKINE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Interleukin-12 - Cellular and Molecular Immunology of an Important Regulatory Cytokine CY NOV 09-12, 1995 CL NEW YORK, NEW YORK SP New York Acad Sci, Genet Inst Inc, Hoffmann La Roche Inc, Boehringer Ingelheim Pharm Inc, Bristol Myers Squibb Pharm Res Inst, Lab Line Instruments Inc, Merck & Co Inc, NCI, Pfizer Inc, Upjohn Co, SmithKline Beecham Pharm, Wyeth Ayerst Res ID INTERFERON-GAMMA-PRODUCTION; SEZARY-SYNDROME; MYCOSIS-FUNGOIDES; STIMULATORY FACTOR; CYTOKINE PRODUCTION; PERIPHERAL-BLOOD; SECRETION; LYMPHOCYTES; ANTITUMOR; PATTERN C1 Univ Penn, Med Ctr, Dept Dermatol, Philadelphia, PA 19104 USA. Med Coll Penn & Hahnemann Univ, Philadelphia Vet Affairs Med Ctr, Dept Dermatol, Philadelphia, PA 19164 USA. Med Coll Penn & Hahnemann Univ, Philadelphia Vet Affairs Med Ctr, Wistar Inst, Philadelphia, PA 19164 USA. RP Rook, AH (reprint author), Univ Penn, Med Ctr, Dept Dermatol, 3400 Spruce St, Philadelphia, PA 19104 USA. FU NCI NIH HHS [1RO1 CA58841, R29 CA55017] NR 26 TC 23 Z9 23 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 USA SN 0077-8923 BN 1-57331-018-2 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 795 BP 310 EP 318 DI 10.1111/j.1749-6632.1996.tb52680.x PG 9 WC Biochemistry & Molecular Biology; Cell Biology; Immunology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Immunology; Science & Technology - Other Topics GA BK04A UT WOS:000070968300032 PM 8958942 ER PT B AU Harris, WH Smith, EJ Goetz, DD AF Harris, WH Smith, EJ Goetz, DD BE Pritchard, DJ TI Bone cement as a seal protecting the femur from the ingress of particulate debris and femoral osteolysis SO INSTRUCTIONAL COURSE LECTURES, VOL 45, 1996 SE AMERICAN ACADEMY OF ORTHOPAEDIC SURGEONS INSTRUCTIONAL COURSE LECTURES LA English DT Proceedings Paper CT 1995 Instructional Course Lectures, at the Annual Meeting of the American-Academy-of-Orthopaedic-Surgeons CY 1995 CL ORLANDO, FL SP Amer Acad Orthopaed Surgeons RP Harris, WH (reprint author), MASSACHUSETTS GEN HOSP,HIP & IMPLANT UNIT,BOSTON,MA 02114, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ACAD ORTHOPAEDIC SURGEONS PI ROSEMONT PA 6300 NORTH RIVER RD, ROSEMONT, IL 60018 BN 0-89203-145-X J9 AAOS INSTR COURS LEC PY 1996 VL 45 BP 183 EP 185 PG 3 WC Orthopedics SC Orthopedics GA BH44V UT WOS:A1996BH44V00021 ER PT B AU Springfield, DS Gebhardt, MC McGuire, MH AF Springfield, DS Gebhardt, MC McGuire, MH BE Pritchard, DJ TI Chondrosarcoma: A review SO INSTRUCTIONAL COURSE LECTURES, VOL 45, 1996 SE AMERICAN ACADEMY OF ORTHOPAEDIC SURGEONS INSTRUCTIONAL COURSE LECTURES LA English DT Proceedings Paper CT 1995 Instructional Course Lectures, at the Annual Meeting of the American-Academy-of-Orthopaedic-Surgeons CY 1995 CL ORLANDO, FL SP Amer Acad Orthopaed Surgeons RP Springfield, DS (reprint author), MASSACHUSETTS GEN HOSP,BOSTON,MA 02114, USA. NR 0 TC 19 Z9 19 U1 0 U2 0 PU AMER ACAD ORTHOPAEDIC SURGEONS PI ROSEMONT PA 6300 NORTH RIVER RD, ROSEMONT, IL 60018 BN 0-89203-145-X J9 AAOS INSTR COURS LEC PY 1996 VL 45 BP 417 EP 424 PG 8 WC Orthopedics SC Orthopedics GA BH44V UT WOS:A1996BH44V00044 ER PT J AU Rice, CA AF Rice, CA TI Premature termination of group therapy: A clinical perspective SO INTERNATIONAL JOURNAL OF GROUP PSYCHOTHERAPY LA English DT Article ID GROUP-PSYCHOTHERAPY; PREDICTORS; BORDERLINE; OUTPATIENT; PATIENT AB Premature terminations are an inevitable if complex aspect of any therapy group, as they are of life. They can be destructive to the life of a group, and damaging to its members. It is possible however, given proper preparation and thoughtful reflection to make such premature endings effective. And sometimes, understanding the wish to leave may change potentially premature endings into turning points in a continuing therapy. I tire two clinical events to illustrate these processes. Premature terminations are described as part of a continuum of ending that include dropouts, early endings, and good-enough terminations. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA. BOSTON UNIV,SCH THEOL,BOSTON,MA 02215. BOSTON INST PSYCHOTHERAPIES INC,BOSTON,MA. SMITH COLL,SCH SOCIAL WORK,NORTHAMPTON,MA 01063. NR 54 TC 2 Z9 2 U1 1 U2 5 PU GUILFORD PUBLICATIONS INC PI NEW YORK PA 72 SPRING STREET, NEW YORK, NY 10012 SN 0020-7284 J9 INT J GROUP PSYCHOTH JI Int. J. Group Psychother. PD JAN PY 1996 VL 46 IS 1 BP 5 EP 23 PG 19 WC Psychology, Clinical SC Psychology GA UA691 UT WOS:A1996UA69100003 PM 8714546 ER PT J AU Horner, MD Freides, D AF Horner, MD Freides, D TI Effects of retinal eccentricity on the lateralized processing of categorical and coordinate spatial relations SO INTERNATIONAL JOURNAL OF NEUROSCIENCE LA English DT Article DE neuropsychology; hemispheric asymmetry; categorical; coordinate; spatial relations; visual information processing ID CEREBRAL HEMISPHERES; REPRESENTATIONS; SPECIALIZATION AB Kosslyn's (1987) hypothesized cerebral hemispheric asymmetries for processing categorical and coordinate spatial relations were explored, using stimuli presented at varying degrees of retinal eccentricity. Thirty-three adult males performed hemifield reaction-time tasks requiring categorical or coordinate judgments. The hypothesized asymmetries were observed when stimuli were presented at 3 degrees of visual angle, but not at 1 or 9 degrees. Nevertheless, correlational analyses supported the existence of separate neural subsystems for categorical and coordinate processing. The results suggest that the model accurately predicts performance asymmetries only under specific conditions. C1 EMORY UNIV,ATLANTA,GA 30322. MED UNIV S CAROLINA,CHARLESTON,SC 29425. RP Horner, MD (reprint author), RALPH H JOHNSON DEPT VET AFFAIRS MED CTR,PSYCHOL SERV 116B,109 BEE ST,CHARLESTON,SC 29401, USA. NR 18 TC 1 Z9 1 U1 1 U2 1 PU GORDON BREACH SCI PUBL LTD PI READING PA C/O STBS LTD PO BOX 90, READING, BERKS, ENGLAND RG1 8JL SN 0020-7454 J9 INT J NEUROSCI JI Int. J. Neurosci. PY 1996 VL 86 IS 1-2 BP 7 EP 13 PG 7 WC Neurosciences SC Neurosciences & Neurology GA UY504 UT WOS:A1996UY50400002 PM 8828055 ER PT J AU Lynch, TJ Kalish, L Mentzer, SJ Decamp, M Strauss, G Sugarbaker, DJ AF Lynch, TJ Kalish, L Mentzer, SJ Decamp, M Strauss, G Sugarbaker, DJ TI Optimal therapy of malignant pleural effusions: Report of a randomized trial of bleomycin, tetracycline, and talc and a meta-analysis SO INTERNATIONAL JOURNAL OF ONCOLOGY LA English DT Article DE malignant pleural effusion; bleomycin; tetracycline; talc; chest tube sclerosis; randomized allocation; meta-analysis ID INTRACAVITARY BLEOMYCIN; MANAGEMENT; CANCER; SECONDARY; BREAST AB A randomized phase III trial of bleomycin, tetracycline and talc following chest tube drainage and a meta-analysis of relative benefit of bleomycin and tetracycline as sclerosing agents were performed to determine the optimal approach to malignant pleural effusion (MPE). Fifty patients were randomized to receive bleomycin (n=16), tetracycline (n=19) or talc (n=16) following chest tube drainage. Treatment groups were balanced for pretreatment characteristics. The study was ended prematurely because of the removal of parenteral tetracycline from the market. Overall, 52% of randomized patients had successful control of effusion 30 days after sclerosis. There were no differences between any of the three treatment groups in terms of 30 day control of effusion, overall survival (6 months), resclerosis rate, pain with sclerosis, fever, or duration of hospitalization (6 days). A meta-analysis was performed using the four previously reported trials of tetracycline vs. bleomycin and revealed a 20.6% advantage to the use of bleomycin (95% C.I. 7.9%-33.3%) (p=0.002). This phase III failed to demonstrate a significant difference between the three agents in terms of control of MPE at 30 days, side effects or survival. However, because of small sample size, this study lacks sufficient power to observe potentially clinically important differences between treatment groups. Inclusion of data from four previous trials in a meta-analysis showed that bleomycin may be superior. The median duration of hospitalization and the overall success rate of all three sclerosing agents in this study argue convincingly that new approaches to palliate MPE are needed. C1 BRIGHAM & WOMENS HOSP,DIV THORAC SURG,BOSTON,MA 02115. BRIGHAM & WOMENS HOSP,DIV HEMATOL ONCOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV CLIN ONCOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV BIOSTAT,BOSTON,MA 02115. NR 25 TC 15 Z9 17 U1 0 U2 3 PU INT JOURNAL ONCOLOGY PI ATHENS PA C/O PROFESSOR D A SPANDIDOS, EDITORIAL OFFICE, 1, S MERKOURI ST, ATHENS 116 35, GREECE SN 1019-6439 J9 INT J ONCOL JI Int. J. Oncol. PD JAN PY 1996 VL 8 IS 1 BP 183 EP 190 PG 8 WC Oncology SC Oncology GA TM024 UT WOS:A1996TM02400025 PM 21544348 ER PT J AU Nolan, SA Fava, M Gotlib, IH AF Nolan, SA Fava, M Gotlib, IH TI Ruminative responses to depression and their prediction of destructive distraction SO INTERNATIONAL JOURNAL OF PSYCHOLOGY LA English DT Meeting Abstract C1 NORTHWESTERN UNIV,EVANSTON,IL. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE, EAST SUSSEX, ENGLAND BN3 2FA SN 0020-7594 J9 INT J PSYCHOL JI Int. J. Psychol. PY 1996 VL 31 IS 3-4 BP 2513 EP 2513 PG 1 WC Psychology, Multidisciplinary SC Psychology GA VE857 UT WOS:A1996VE85701418 ER PT J AU Nolan, SA Gotlib, IH Fava, M AF Nolan, SA Gotlib, IH Fava, M TI Ruminative responses to depression and anxiety: A question of specificity SO INTERNATIONAL JOURNAL OF PSYCHOLOGY LA English DT Meeting Abstract C1 NORTHWESTERN UNIV,EVANSTON,IL. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE, EAST SUSSEX, ENGLAND BN3 2FA SN 0020-7594 J9 INT J PSYCHOL JI Int. J. Psychol. PY 1996 VL 31 IS 3-4 BP 54146 EP 54146 PG 1 WC Psychology, Multidisciplinary SC Psychology GA VE857 UT WOS:A1996VE85703547 ER PT J AU Ling, CC Smith, AR Hanlon, AL Owen, JB Brickner, TJ Hanks, GE AF Ling, CC Smith, AR Hanlon, AL Owen, JB Brickner, TJ Hanks, GE TI Treatment planning for carcinoma of the cervix: A patterns of care study report SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article DE radiation treatment; gynecological cancer; treatment standards ID NATIONAL PRACTICE; DOSE DISTRIBUTION; UTERINE CERVIX; CANCER; BLADDER AB Purpose: The Patterns of Care Study (PCS) of patients treated in 1988-89 included ''patterns of treatment planning'' for radiotherapy of carcinoma of the uterine cervix. A Consensus Committee of radiation physicists and oncologists established current guidelines and developed questionnaires to assess the treatment planning process (i.e., the general structure, methodology, and tools) of institutions involved in the Patterns of Care Study. This paper reports the findings of the assessment. Methods and Materials: The PCS surveyed 73 radiotherapy facilities, of which 21 are academic institutions (AC), 26 hospital-based facilities (HB), and 26 free-standing centers (FS). In total, 242 cases were assessed with 39% from academic centers, 33% from hospital-based centers, and 28% from free-standing centers. The survey collected treatment planning information such as the use of computed tomography (CT), simulation procedure, contouring of patient outline, tumor or target delineation, identification of critical structures, method of dose prescription (point or isodose), etc. Data was also obtained concerning implant boosts, e.g., radioisotope used, use of midline block for external beam treatment, availability of remote afterloader, practice of interstitial implants, combination with hyperthermia, etc. Results: There is a high degree of compliance relative to the basic treatment planning standards. For example, 171 cases (out of 173) from AC and HE institutions included simulation and 169 used port film; for cases from FS centers, 61 out of 69 involved simulation and 66 out of 69 included port film. Most institutions used linacs (231 out of 242); in five cases, Co-60 units and in six cases betatron was used. In terms of treatment planning, 53% used skin contours, but only 14% had target volume delineation, with AC and HE being slightly more conscientious in these efforts. Critical organs did not appear to be explicitly considered in external beam treatment planning, with only 3% outlining the bladder, 5% the rectum, and less than 1% the small bowel. Only 11% of the centers used CT in treatment planning, and none reported the use of magnetic resonance imaging (MRI). For patients receiving implants, about 40% had midline blocking during external beam treatment, of which one out of three were shielded by standard blocks and two out of three with customized ones. About 11% of the patients receiving implants were treated with remote afterloading devices, 5% received interstitial implants, and none were treated in combination with hyperthermia. Conclusion: The treatment planning aspects of radiotherapy of carcinoma of the cervix have been established by this Patterns of Care Study Survey. There is a high level of uniformity in the approach. Some variations exist among centers in the different strata. C1 AMER COLL RADIOL,PHILADELPHIA,PA 19107. MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. ST FRANCIS HOSP,TULSA,OK. FOX CHASE CANC CTR,PHILADELPHIA,PA 19111. FU NCPDCID CDC HHS [NCI-N01-CM-87285] NR 14 TC 6 Z9 6 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0360-3016 J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD JAN 1 PY 1996 VL 34 IS 1 BP 13 EP 19 DI 10.1016/0360-3016(96)80544-X PG 7 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA TN909 UT WOS:A1996TN90900002 PM 12118541 ER PT J AU Lewis, AM Su, M Doty, J Chen, Y Pardo, FS AF Lewis, AM Su, M Doty, J Chen, Y Pardo, FS TI Relationship between intrinsic radiation sensitivity and metastatic potential SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article; Proceedings Paper CT 36th Annual ASTRO Meeting CY OCT, 1994 CL SAN FRANCISCO, CA SP Amer Soc Therapeut Radiol & Oncol DE metastatic potential; intrinsic radiation sensitivity; genomic instability; H-ras; E1a; v-myc ID HUMAN CELL-LINES; H-RAS; INVITRO RADIOSENSITIVITY; SURVIVAL CURVES; HUMAN-TUMORS; V-MYC; ONCOGENES; CANCER; RADIORESISTANCE; TRANSFORMATION AB Purpose: Prior studies emphasized genetic modulation of tumorigenicity, and experimental metastatic potential in cells transfected with oncogenes. Whether the intrinsic radiaton sensitivity of cells might correlate with parallel changes in metastatic potential is unknown. Methods and Materials: Rat embryo cells (REC) were transfected with the following oncogenes, and where appropriate, with corresponding selection markers: pCMVneopEJ6.6ras, pEJ6.6ras/v-myc, pE1a, and pEJ6.6ras/E1a. Individual transfectant clones and corresponding pooled cellular populations were propagated in selective medium. In vitro cellular radiation sensitivity was determined via clonogenic assays, a minimum of three, by standard techniques and individual SF2 and MID parameters determined. Tumorigenicity was defined as the number of tumors forming following the injection of 1 x 10(5)-1 x 10(6) cells into the axillary pouch of three different strains of immune-deficient mice. Animals were killed once resultant tumors reached a maximum size of 1.5-2.0 cm in maximum diameter. For determination of experimental metastatic potential, between 1 x 10(5)-1 x 10(6) cells were injected into the tail veins of litter-matched sibling mice in parallel to the tumorigenicity studies. Results: Radiobiologic studies indicate similar levels of radiation sensitivity among REC, mock-transfected REC, E1a, and combined E1a/ras transfectants. pEJ6.6ras, and combined ras/myc transfected pooled cellular populations demonstrated increases in radiation resistance when compared to the pooled radiobiologic data from untransfected and mock-transfected corresponding pooled cellular populations (p <0.05, two-tailed test, SF2, MID). Rat embryo cells, Ela, and mock-transfectants were relatively radiation sensitive and nontumorigenic. pE1a/ras was tumorigenic but demonstrated relatively low experimental metastatic potential. Ras, and ras/myc transfectants, demonstrated similar; levels of experimental metastatic potential on lung colonization assays. Conclusions: A good correlation exists between the intrinsic radiation sensitivity and the experimental metastatic potential of transfected REC. The highest levels of radiation resistance in vitro and experimental metastatic potential in vivo were found among REC transfected with ras/myc or activated ras alone. E1a/ras cotransfected cellular populations, although tumorigenic, were relatively radiation sensitive and nonmetastatic. Further study is needed to formulate a mechanistic :explanation for the intriguing correlation between intrinsic radiation sensitivity in vitro and metastatic potential in vivo. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT RADIAT ONCOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT RADIAT ONCOL,LAB MOLEC TUMOR RADIAT BIOL,BOSTON,MA. NR 38 TC 9 Z9 10 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0360-3016 J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD JAN 1 PY 1996 VL 34 IS 1 BP 103 EP 110 DI 10.1016/0360-3016(95)02008-X PG 8 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA TN909 UT WOS:A1996TN90900014 PM 12118537 ER PT J AU Ling, SM Flynn, DF AF Ling, SM Flynn, DF TI Results of the 1993 Association of Residents in Radiation Oncology survey SO INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS LA English DT Article DE Association of Residents in Radiation Oncology (ARRO); resident survey; radiation oncology training AB In 1993, the Association of Residents in Radiation Oncology (ARRO) conducted its tenth annual survey of all residents training in radiation oncology in the United States. The characteristics of current residents are described. Factors influencing the choice of Radiation Oncology as a medical specialty, and posttraining career plans were identified. Residents raised issues on the adequacy of training, problems in work routine, and expressed concerns about board certification and recertification, and about decreased future practice opportunities. C1 MASSACHUSETTS GEN HOSP, DEPT RADIAT ONCOL, BOSTON, MA 02114 USA. RP Ling, SM (reprint author), UNIV CALIF SAN FRANCISCO HOSP, DEPT RADIAT ONCOL L75, 505 PARNASSUS ST, SAN FRANCISCO, CA 94143 USA. NR 11 TC 16 Z9 16 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0360-3016 EI 1879-355X J9 INT J RADIAT ONCOL JI Int. J. Radiat. Oncol. Biol. Phys. PD JAN 1 PY 1996 VL 34 IS 1 BP 221 EP 226 DI 10.1016/0360-3016(95)02026-8 PG 6 WC Oncology; Radiology, Nuclear Medicine & Medical Imaging SC Oncology; Radiology, Nuclear Medicine & Medical Imaging GA TN909 UT WOS:A1996TN90900030 PM 12118555 ER PT S AU Miura, M Yuan, JY AF Miura, M Yuan, JY BE Suzuki, K Bond, JS TI Regulation of programmed cell death by interleukin-1 beta-coverting enzyme family of proteases SO INTRACELLULAR PROTEIN CATABOLISM SE ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY LA English DT Proceedings Paper CT 10th International Conference on Intracellular Protein Catabolism CY OCT 30-NOV 03, 1994 CL TOKYO, JAPAN SP Commemorat Assoc Japan World Exposit 1970, Fdn Adv Int Sci, Pharm Soc Japan, Japanese Agr Chem Soc Japan, Japanese Biochem Soc, Japanese Soc Cell Biol, Molec Biol Soc Japan, Protein Engn Soc Japan ID CAENORHABDITIS-ELEGANS; C-ELEGANS; CONVERTING ENZYME; SERINE PROTEASES; LYMPHOCYTES-T; GENE CED-3; INTERLEUKIN-1; APOPTOSIS; PROTEIN; NEMATODE C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. RP Miura, M (reprint author), MASSACHUSETTS GEN HOSP EAST,CARDIOVASC RES CTR,BOSTON,MA 02129, USA. NR 56 TC 4 Z9 4 U1 0 U2 0 PU PLENUM PRESS DIV PLENUM PUBLISHING CORP PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 SN 0065-2598 BN 0-306-45201-4 J9 ADV EXP MED BIOL JI Adv.Exp.Med.Biol. PY 1996 VL 389 BP 165 EP 172 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA BF18P UT WOS:A1996BF18P00020 PM 8861007 ER PT J AU Witte, RS Hsieh, P Elson, P Oken, MM Trump, DL AF Witte, RS Hsieh, P Elson, P Oken, MM Trump, DL TI A phase II trial of amonafide, caracemide, and homoharringtonine in the treatment of patients with advanced renal cell cancer SO INVESTIGATIONAL NEW DRUGS LA English DT Article DE amonafide; caracemide; homoharringtonine; renal cell cancer ID COOPERATIVE-ONCOLOGY-GROUP; ACUTE MYELOGENOUS LEUKEMIA; INTERFERON ALFA-2A; CARCINOMA; RECURRENT; INTERLEUKIN-2; VINBLASTINE; LEUKOCYTE; SURVIVAL; THERAPY AB Forty-eight previously untreated, ambulatory patients with advanced or unresectable renal carcinoma were treated with either amonafide (17 patients), caracemide (17 patients), or homoharringtonine (14 patients). No objective responses were observed in any of the treatment cohorts. Amonafide and caracemide were well tolerated with no unexpected toxicities. One patient each died of pulmonary thromboembolism and sepsis with severe metabolic acidosis on the homoharringtonine arm. An additional 4 patients experienced grade 4 complications including myelosuppression, neurologic dysfunction, and respiratory failure. These severe and unexpected complications caused early termination of accrual to the homoharringtonine arm of the study. These agents have no activity in the treatment of advanced renal cell carcinoma. C1 DANA FARBER CANC INST,DIV BIOSTAT,BOSTON,MA 02115. CLEVELAND CLIN,CTR CANC,CLEVELAND,OH 44106. VIRGINIA PIPER CANC INST,MINNEAPOLIS,MN. UNIV PITTSBURGH,PITTSBURGH,PA 15260. RP Witte, RS (reprint author), GUNDERSEN LUTHERAN,GUNDERSEN CLIN,1836 S AVE,LA CROSSE,WI 54601, USA. FU NCI NIH HHS [CA 21076, CA 18653, CA 23318] NR 30 TC 12 Z9 12 U1 0 U2 0 PU KLUWER ACADEMIC PUBL PI DORDRECHT PA SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS SN 0167-6997 J9 INVEST NEW DRUG JI Invest. New Drugs PY 1996 VL 14 IS 4 BP 409 EP 413 PG 5 WC Oncology; Pharmacology & Pharmacy SC Oncology; Pharmacology & Pharmacy GA WK131 UT WOS:A1996WK13100011 PM 9157078 ER PT J AU Silva, JA Leong, GB AF Silva, JA Leong, GB TI The relation of Cotard's syndrome to delusional misidentification SO ISRAEL JOURNAL OF PSYCHIATRY AND RELATED SCIENCES LA English DT Article ID CAPGRAS SYNDROME; TOMOGRAPHY; DOUBLES; ATROPHY; SELF AB A case of Cotard's syndrome with face processing deficits is described. The phenomenology of Cotard's syndrome may be an expression of underlying visual processing deficits, including abnormalities in face perception processing. Phenomenologic and neurobiologic characteristics of this case are similar to those found among delusional misidentification syndromes. This similarity suggests areas for further study that may lead to a better understanding of the relation of phenomenology to neurobiology in Cotard's syndrome. C1 UNIV TEXAS,HLTH SCI CTR,DEPT PSYCHIAT,SAN ANTONIO,TX. UNIV MISSOURI,SCH MED,DEPT PSYCHIAT & NEUROL,COLUMBIA,MO 65211. HARRY S TRUMAN MEM VET HOSP,PSYCHIAT SERV,COLUMBIA,MO 65201. RP Silva, JA (reprint author), AUDIE L MURPHY MEM VET ADM MED CTR,PSYCHIAT SERV 116A,7400 MERTON MINTER BLVD,SAN ANTONIO,TX 78284, USA. NR 33 TC 4 Z9 4 U1 1 U2 6 PU GEFEN PUBL HOUSE LTD PI JERUSALEM PA PO BOX 6056, JERUSALEM 91060, ISRAEL SN 0333-7308 J9 ISRAEL J PSYCHIAT JI Isr. J. Psychiatr. Relat. Sci. PY 1996 VL 33 IS 3 BP 188 EP 193 PG 8 WC Psychiatry SC Psychiatry GA VQ278 UT WOS:A1996VQ27800006 PM 9009518 ER PT J AU Simberkoff, MS Hartigan, PM Hamilton, JD Day, PL Diamond, GR Dickinson, GM Drusano, GL Egorin, MJ George, WL Gordin, FM Hawkes, CA Jensen, PC Klimas, NG Labriola, AM Lahart, CJ OBrien, WA Oster, CN Weinhold, KJ Wray, NP Pazner, SBZ AF Simberkoff, MS Hartigan, PM Hamilton, JD Day, PL Diamond, GR Dickinson, GM Drusano, GL Egorin, MJ George, WL Gordin, FM Hawkes, CA Jensen, PC Klimas, NG Labriola, AM Lahart, CJ OBrien, WA Oster, CN Weinhold, KJ Wray, NP Pazner, SBZ TI Long-term follow-up of symptomatic HIV-infected patients originally randomized to early versus later zidovudine treatment: Report of a veterans affairs cooperative study SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES LA English DT Article DE zidovudine; human immunodeficiency virus; opportunistic infections; survival ID IMMUNODEFICIENCY-VIRUS INFECTION; PLACEBO-CONTROLLED TRIAL; CUBIC MILLIMETER; DOUBLE-BLIND; THERAPY; AZT; SENSITIVITY; EFFICACY; SURVIVAL; AIDS AB Following a 4-year controlled trial comparing early and later zidovudine treatment, we conducted an additional 3-year follow-up. Of the original 338 patients, 275 participated. Clinical outcome measures were AIDS and death. In the early therapy group (n = 170), 67 patients progressed to AIDS compared with 85 in the later therapy group (n = 168); the relative risk (RR) comparing early with later therapy was 0.72 (95% confidence interval [CI] 0.52-0.99; p = 0.044). The early therapy group had 74 deaths compared with 73 in the later therapy (RR = 0.98; 95% CI, 0.71-1.36; p = 0.91). The early group had a peak CD4+ count increase at 1-2 months and a delay of 1 year before CD4+ counts fell below baseline. For patients who received zidovudine for more than the median duration (20.3 months) before their first AIDS diagnosis, the RR for death was 2.08 (95% CI, 1.36-3.19, p = 0.001). Additional factors independently associated with poor prognosis following AIDS were a CD4+ count of <100 cells/mm(3) and increased severity of the first AIDS diagnosis, whereas use of another antiretroviral agent was associated with improved survival. We conclude that early zidovudine therapy delays progression to AIDS but does not affect survival. Patients who progress to AIDS while on prolonged zidovudine monotherapy many benefit from a change to other antiretroviral therapy(ies). C1 DEPT VET AFFAIRS MED CTR, BALTIMORE, MD USA. DEPT VET AFFAIRS MED CTR, DURHAM, NC USA. DEPT VET AFFAIRS MED CTR, HOUSTON, TX USA. DEPT VET AFFAIRS MED CTR, LOS ANGELES, CA USA. DEPT VET AFFAIRS MED CTR, MIAMI, FL USA. DEPT VET AFFAIRS MED CTR, SAN FRANCISCO, CA USA. DEPT VET AFFAIRS MED CTR, WASHINGTON, DC USA. ALBANY MED COLL, ALBANY, NY USA. DUKE UNIV, DURHAM, NC USA. WALTER REED ARMY MED CTR, BETHESDA, MD USA. VET ADM COORDINATING CTR, NEW HAVEN, CT USA. VET ADM COORDINATING CTR, ALBUQUERQUE, NM USA. RP Simberkoff, MS (reprint author), VET ADM MED CTR, INFECT DIS SECT, 423 E 23RD ST, NEW YORK, NY 10010 USA. NR 30 TC 15 Z9 15 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1525-4135 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. PY 1996 VL 11 IS 2 BP 142 EP 150 PG 9 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA TR348 UT WOS:A1996TR34800005 PM 8556396 ER PT J AU Manome, Y Yao, XJ Kufe, DW Cohen, EA Fine, HA AF Manome, Y Yao, XJ Kufe, DW Cohen, EA Fine, HA TI Selective effects of DNA damaging agents on HIV long terminal repeat activation and virus replication in vitro SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY LA English DT Article DE human immunodeficiency virus (HIV); DNA damage; long terminal repeat (LTR); radiation; ultraviolet light; chemotherapy ID HUMAN-IMMUNODEFICIENCY-VIRUS; VIRAL GENE-EXPRESSION; HUMAN-CELLS; HTLV-I; ULTRAVIOLET-IRRADIATION; METHYL METHANESULFONATE; LEUKEMIA-CELLS; CROSS-LINKING; REPAIR; TYPE-1 AB Much attention has recently focused on the observation that UV light can activate the long terminal repeat (LTR) of the human immunodeficiency virus (HIV). Although the mechanism of LTR activation remains obscure, several lines of investigation have suggested that it is a result of activation of the NF-kappa B transcription factor(s) following signaling events related to generalized DNA damage. In this report, we present data demonstrating that HIV LTR activation is not a general consequence of cellular DNA damage, but rather a process unique to specific genotoxic stimuli, and that it does not necessarily depend on activation of NF-kappa B. Furthermore, we demonstrate that several of these agents can significantly increase HIV replication and accelerate CD4-positive lymphocyte cytotoxicity in vitro. These findings, therefore, could have clinical significance to AIDS patients with malignancies who are undergoing radiotherapy and chemotherapy. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV CANC PHARMACOL,BOSTON,MA 02115. UNIV MONTREAL,FAC MED,DEPT MICROBIOL & IMMUNOL,LAB RETROVIROL HUMAINE,MONTREAL,PQ H3C 3J7,CANADA. FU NCI NIH HHS [CA 38493, K11 CA01467-03, CA 55241] NR 47 TC 4 Z9 4 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1077-9450 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. PY 1996 VL 11 IS 2 BP 109 EP 116 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA TR348 UT WOS:A1996TR34800001 PM 8556392 ER PT J AU Park, IW Sodroski, J AF Park, IW Sodroski, J TI Targeting a foreign protein into virion particles by fusion with the Vpx protein of simian immunodeficiency virus SO JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES AND HUMAN RETROVIROLOGY LA English DT Article DE Vpx; Vpr; simian immunodeficiency virus ID VIRAL REPLICATION; TYPE-2; GENE; MACROPHAGES; PRODUCT AB The Vpx and Vpr proteins of the primate immunodeficiency viruses are stoichiometrically incorporated into virion particles. The chloramphenicol acetyltransferase (CAT) enzyme, when fused to a sufficient portion of the simian immunodeficiency virus (SIV mac239) Vpx protein, was incorporated into virions and retained enzymatic activity. An analysis of the replication of this virus compared with the replication of revertants and control viruses encoding nonpackageable Vpx-CAT fusion proteins suggested that the observed delay in replication was due to cis-acting effects of the CAT gene insertion rather than to the presence of the Vpx-CAT fusion protein in the virions. These studies indicate that, in host cells where Vpx and Vpr function is not required for efficient SIV mac replication, functional enzymes can be incorporated into virions by fusion with the Vpx protein. This approach could be utilized for study of the function and localization of Vpx and/or Vpr proteins during virus replication and for attempts to disrupt virus replication by the incorporation of foreign proteins. C1 DANA FARBER CANC INST,DEPT HUMAN RETROVIROL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. HARVARD UNIV,SCH PUBL HLTH,DEPT CANC BIOL,BOSTON,MA 02115. FU NCI NIH HHS [P30 CA06516]; PHS HHS [P30 A128691, R01 A129333] NR 22 TC 5 Z9 5 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1077-9450 J9 J ACQ IMMUN DEF SYND JI J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. PY 1996 VL 11 IS 4 BP 341 EP 350 PG 10 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA UC833 UT WOS:A1996UC83300004 PM 8601220 ER PT J AU Compton, PA Ling, W Wesson, DR Charuvastra, VC Wilkins, J AF Compton, PA Ling, W Wesson, DR Charuvastra, VC Wilkins, J TI Urine toxicology as an outcome measure in drug abuse clinical trials: Must every sample be analyzed? SO JOURNAL OF ADDICTIVE DISEASES LA English DT Article AB Clinical trials designed to establish the effectiveness of a pharmacotherapy for the treatment of drug abuse typically call for the collection and analysis of three urine samples per week to detect changes in drug use patterns. Examination of over 16,500 urine samples collected from 225 subjects during a one year buprenorphine/methadone clinical trial indicates that analysis of one weekly urine sample from those collected on a three-times-per-week fixed schedule provides essentially the same outcome information as analysis of all three weekly urines. Further, the percent of opiate-positive samples is constant across weekday, indicating that a single urine, randomly selected from those collected each week, is a valid indicator of treatment performance. C1 UNIV CALIF LOS ANGELES,SCH NURSING,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP Compton, PA (reprint author), LOS ANGELES ADDICT TREATMENT RES CTR,10350 SANTA MONICA BLVD,SUITE 340,LOS ANGELES,CA 90211, USA. FU NIDA NIH HHS [R18-DA006082, T32-DA07272] NR 13 TC 4 Z9 4 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 1055-0887 J9 J ADDICT DIS JI J. Addict. Dis. PY 1996 VL 15 IS 2 BP 85 EP 92 DI 10.1300/J069v15n02_07 PG 8 WC Substance Abuse SC Substance Abuse GA UE197 UT WOS:A1996UE19700007 PM 8704003 ER PT J AU Knoefel, WT Kollias, N Rattner, DW Nishioka, NS Warshaw, AL AF Knoefel, WT Kollias, N Rattner, DW Nishioka, NS Warshaw, AL TI Reflectance spectroscopy of pancreatic microcirculation SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE hemoglobin oxygen saturation; hemoglobin content; pancreas ID HEPATIC HEMODYNAMICS; OXYGEN-CONSUMPTION; SPECTROPHOTOMETRY; LIVER; HEMOPERFUSION AB A technique employing diffuse reflectance spectroscopy (DRS) is described to assess and mirror dynamic changes of pancreatic tissue perfusion. An especially designed reflectance spectrophotometer was initially used to derive the quantitative relation between hemoglobin concentration ([Hb]) and reflectance measurements in vitro. Over a wide range of scattering related to the medium in which the measurements were made (scattering coefficient: 6.5-13 cm(-1)), a close, direct correlation existed with a slope of 0.376 +/- 0.012. In Sprague-Dawley rats under general anesthesia, the pancreas was isolated in situ and perfused with graded infusions of hemoglobin solutions. A correlation, comparable to the in vitro setting, was found between a [Hb] of 0 and 14 g/dl in the perfusate with slopes of 0.0037 and 0.0035. Changes in perfusion induced by adrenergic drugs produced changes in hemoglobin oxygen saturation and [Hb] that correspond with measured alterations of systemic arterial pressure and aortic blood flow. We conclude that diffuse reflectance spectroscopy reliably provides data on intrapancreatic hemoglobin oxygen saturation and [Hb] that can be a valuable tool for minimally invasive on-line evaluation of these aspects of pancreatic perfusion in the rat. This newly designed device is superior to previously used ones in that it analyzes the entire spectrum and therefore can account for changes in scattering that are very likely to occur with pathophysiological alterations such as edema formation. C1 MASSACHUSETTS GEN HOSP,DEPT SURG,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT SURG,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT DERMATOL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02114. NR 27 TC 42 Z9 42 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD JAN PY 1996 VL 80 IS 1 BP 116 EP 123 PG 8 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA UE823 UT WOS:A1996UE82300016 PM 8847291 ER PT J AU Hurford, WE Hochachka, PW Schneider, RC Guyton, GP Stanek, KS Zapol, DG Liggins, GC Zapol, WM AF Hurford, WE Hochachka, PW Schneider, RC Guyton, GP Stanek, KS Zapol, DG Liggins, GC Zapol, WM TI Splenic contraction, catecholamine release, and blood volume redistribution during diving in the Weddell seal SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE spleen; blood hematocrit; ultrasonography; diving mammals; Leptonychotes weddelli; breath-hold diving ID BEHAVIOR; SPLEEN; HEMATOCRIT; TENSIONS; EXERCISE; APNEA AB The spleen of the Weddell seal (Leptonychotes weddelli) may contract and inject red blood cells (RBCs) into the peripheral circulation during diving, but evidence for this hypothesis is indirect. Accordingly, we measured splenic dimensions by ultrasonography, plasma catecholamine concentrations, hemoglobin concentration, and hematocrit in five Weddell seals before and after intravenous epinephrine during halothane anesthesia and while awake at the surface after voluntary dives. Spleen size was reduced immediately after epinephrine injection or after the seal surfaced. Within the first 2 min after the seal surfaced, cephalocaudal splenic length was 71 +/- 2% (mean +/- SD; P < 0.05) and splenic thickness was 71 +/- 4% (P < 0.05) of the maximal resting values. Splenic size increased (halftime = 6-9 min) after the seal surfaced and was inversely correlated with plasma epinephrine and norepinephrine concentrations. Hemoglobin concentration increased from 17.5 +/- 5.3 g/dl (measured during general anesthesia) to 21.9 +/- 3.7 g/dl (measured in the first 2 min after surfacing). At these same times, the hematocrit increased from 44 +/- 12 to 55 +/- 8%. These values decreased (half-time = 12-16 min) after the seal surfaced. We estimate 20.1 liters of RBCs were sequestered at rest, presumably in the spleen, and released either on epinephrine injection or during diving. Catecholamine release and splenic contraction appear to be an integral part of the voluntary diving response of Weddell seals. C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. UNIV BRITISH COLUMBIA,DEPT ZOOL,VANCOUVER,BC V6T 2A9,CANADA. NATL WOMENS HOSP,POSTGRAD SCH OBSTET & GYNAECOL,AUCKLAND 3,NEW ZEALAND. RP Hurford, WE (reprint author), MASSACHUSETTS GEN HOSP,DEPT ANESTHESIA,BOSTON,MA 02114, USA. RI Hurford, William/G-6386-2013 OI Hurford, William/0000-0003-1201-0313 NR 34 TC 72 Z9 73 U1 2 U2 28 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD JAN PY 1996 VL 80 IS 1 BP 298 EP 306 PG 9 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA UE823 UT WOS:A1996UE82300040 PM 8847318 ER PT J AU Frautschi, JR Eberhart, RC Hubbell, JA Clark, BD Gelfand, JA AF Frautschi, JR Eberhart, RC Hubbell, JA Clark, BD Gelfand, JA TI Alkylation of cellulosic membranes results in reduced complement activation SO JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION LA English DT Article DE hemodialysis membrane; complement activation; polymer; surface modification ID BLOOD COMPATIBILITY; HEMODIALYSIS AB 4-Vinyl pyridine was grafted to the surface of the cellulosic membrane Cuprophan, and subsequently alkylated with both C10 and C16 aliphatic chains. Complement activation of heparinized human blood, corrected for anaphylatoxin adhesion, was measured by radioimmunoassay. The surface treatments both yielded substantial reductions in C5a activity, with a lessor reduction in C3a and C4a activity. Alkylation with 10 and 16 carbon chains resulted both in enhancements of albumin adsorption and stability. These enhancements as well as the reductions in complement activation were statistically indistinguishable between the two treatments. The reduction in complement activation was influenced more by adsorption of endogenous albumin and possibly by the vinyl pyridine graft, than the removal of surface active hydroxyl groups from Cuprophan. C1 UNIV TEXAS,SW MED SCH,DEPT BIOMED ENGN,DALLAS,TX 75235. UNIV TEXAS,DEPT CHEM ENGN,AUSTIN,TX 78712. TUFTS UNIV NEW ENGLAND MED CTR,DEPT MED,BOSTON,MA 02111. HARVARD UNIV,SCH MED,DEPT SURG,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. RP Frautschi, JR (reprint author), CARBOMED,1300 AE ANDERSON LANE,AUSTIN,TX 78752, USA. RI Hubbell, Jeffrey/A-9266-2008 OI Hubbell, Jeffrey/0000-0003-0276-5456 NR 21 TC 8 Z9 8 U1 0 U2 3 PU VSP BV PI ZEIST PA PO BOX 346, 3700 AH ZEIST, NETHERLANDS SN 0920-5063 J9 J BIOMAT SCI-POLYM E JI J. Biomater. Sci.-Polym. Ed. PY 1996 VL 7 IS 8 BP 707 EP 714 DI 10.1163/156856296X00471 PG 8 WC Engineering, Biomedical; Materials Science, Biomaterials; Polymer Science SC Engineering; Materials Science; Polymer Science GA TW718 UT WOS:A1996TW71800006 PM 8639479 ER PT J AU Burleson, AC Levine, RA Yoganathan, AP AF Burleson, AC Levine, RA Yoganathan, AP TI A model based on dimensional analysis for non-invasive quantification of valvular regurgitation under confined and impinging conditions SO JOURNAL OF BIOMECHANICS LA English DT Article ID NONINVASIVE QUANTIFICATION; ULTRASOUND; INVITRO AB The most descriptive measure of valvular insufficiency is the regurgitant volume. Current techniques for measuring it, however, are invasive and semi-quantitative at best. Cape and colleagues have recently developed a non-invasive technique for the quantitation of regurgitant hows corresponding to free jets cases. This technique is, unfortunately, not applicable to cases of jets constrained and/or impinging on the atrial walls as observed in many cases of mitral regurgitation. The purpose of this paper was therefore to develop an equation based on dimensional analysis, for calculating peak regurgitant dow rates from quantities than can be measured by Doppler ultrasound/echocardiography. The result is an equation for flow rate, Q(o), as a function of orifice velocity, U-o, a downstream centerline velocity, U-m, at a distance, x, from the orifice, the diameter of the receiving chamber, D-c, and the impingement height, H: Q(o)=(pi U-o/4)[a(U-o/U-m)H(c)D(c)(d)x(e)]([2/(c+d+e)]), where a, c, d and e can be found by multiple linear regressions on pulsed Doppler jet centerline velocity data. The assumptions made in the derivation are such that they should be physiologically applicable. The advantage of this method compared to the previous one is its theoretical justification and ability to quantify accurately peak regurgitant dow rate, and total regurgitant volume. C1 GEORGIA INST TECHNOL,SCH CHEM ENGN,CARDIOVASC FLUID MECH LAB,ATLANTA,GA 30332. HARVARD UNIV,SCH MED,DEPT MED,MASSACHUSETTS GEN HOSP,CARDIAC ULTRASOUND LAB,BOSTON,MA. FU NHLBI NIH HHS [HL45485] NR 25 TC 1 Z9 1 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0021-9290 J9 J BIOMECH JI J. Biomech. PD JAN PY 1996 VL 29 IS 1 BP 99 EP 102 DI 10.1016/0021-9290(95)00015-1 PG 4 WC Biophysics; Engineering, Biomedical SC Biophysics; Engineering GA TM444 UT WOS:A1996TM44400012 PM 8839022 ER PT J AU McKee, MD Jupiter, JB Bamberger, HB AF McKee, MD Jupiter, JB Bamberger, HB TI Coronal shear fractures of the distal end of the humerus SO JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME LA English DT Article AB We identified a shear fracture of the distal articular surface of the humerus, with anterior and proximal displacement of the capitellum and a portion of the trochlea, in six patients (five female and one male). The average age of the patients was thirty-eight years (range, ten to sixty-three years). Each fracture was the result of a fall from a standing height. A characteristic radiographic abnormality, which we have termed the double-are sign, was seen on the lateral radiograph of each patient and represented the subchondral bone of the displaced capitellum and the lateral trochlear ridge. All patients were managed with open reduction, internal fixation, and early motion of the elbow. The average duration of follow-up was twenty-two months (range, eighteen to twenty-six months). The fracture united in all patients at an average of six weeks (range, four to nine weeks), without radiographic evidence of osteonecrosis of the fracture fragment. Flexion of the elbow averaged 141 degrees (range, 130 to 150 degrees), with an average flexion contracture of 15 degrees (range, 0 to 40 degrees). Pronation of the forearm averaged 83 degrees, and supination averaged 84 degrees. All patients had a good or excellent functional result, according to the elbow-rating scale of Broberg and Morrey. C1 MASSACHUSETTS GEN HOSP,ORTHOPAED HAND SERV,BOSTON,MA 02114. OHIO UNIV,GRANDVIEW HOSP,DAYTON,OH. NR 20 TC 78 Z9 92 U1 0 U2 2 PU JOURNAL BONE JOINT SURGERY INC PI NEEDHAM PA 20 PICKERING ST, NEEDHAM, MA 02192 SN 0021-9355 J9 J BONE JOINT SURG AM JI J. Bone Joint Surg.-Am. Vol. PD JAN PY 1996 VL 78A IS 1 BP 49 EP 54 PG 6 WC Orthopedics; Surgery SC Orthopedics; Surgery GA TQ797 UT WOS:A1996TQ79700007 PM 8550679 ER PT J AU Springfield, DS Gebhardt, MC McGuire, MH AF Springfield, DS Gebhardt, MC McGuire, MH TI Chondrosarcoma: A review SO JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME LA English DT Review ID MESENCHYMAL CHONDROSARCOMA; CLINICOPATHOLOGIC ANALYSIS; BONE; RADIOTHERAPY; EXPERIENCE C1 CREIGHTON UNIV,SCH MED,OMAHA,NE 68131. RP Springfield, DS (reprint author), MASSACHUSETTS GEN HOSP,32 FRUIT ST,GRB 606,BOSTON,MA 02114, USA. NR 38 TC 25 Z9 28 U1 0 U2 0 PU JOURNAL BONE JOINT SURGERY INC PI NEEDHAM PA 20 PICKERING ST, NEEDHAM, MA 02192 SN 0021-9355 J9 J BONE JOINT SURG AM JI J. Bone Joint Surg.-Am. Vol. PD JAN PY 1996 VL 78A IS 1 BP 141 EP 149 PG 9 WC Orthopedics; Surgery SC Orthopedics; Surgery GA TQ797 UT WOS:A1996TQ79700019 ER PT J AU DAmbra, M AF DAmbra, M TI Restoration of the normal coagulation process: Advances in therapies to antagonize heparin SO JOURNAL OF CARDIOVASCULAR PHARMACOLOGY LA English DT Article; Proceedings Paper CT 1st Annual Meeting of the Cardiopulmonary-Bypass-Consensus-Panel CY OCT 19-21, 1994 CL NAPA VALLEY, CA SP Cardiopulmonary Bypass Consensus Panel DE protein C; protein S; thrombomodulin; serine protease inhibitor; recombinant platelet factor 4; cardiopulmonary ID PLATELET FACTOR-IV; PROTEIN-C DEFICIENCY; HUMAN MEGAKARYOCYTOPOIESIS INVITRO; CARDIOPULMONARY BYPASS; PROTAMINE COMPLEXES; INHIBITOR; APROTININ; THROMBOSIS; ANTICOAGULATION; NEUTRALIZATION AB A number of naturally occurring anticoagulants exist that preserve normal blood fluidity and limit blood clot formation to vascular injury sites, thus acting as regulators of hemostasis. The protein C/protein S pathway is one system that acts to modulate thrombin formation. The activation of protein C by thrombin is accelerated more than 1,000-fold at the endothelial surface by thrombomodulin localized on the endothelial cell. Activated protein C then binds to its co-factor, protein S, and the protein C/protein S complex exerts its antithrombotic function by inactivating the coagulation factors Va and VIIIa. Patients deficient in protein C and protein S may be particularly vulnerable to thrombotic events after cardiac surgery. In addition, several studies suggest that reductions in protein C and protein S concentrations, as well as thrombomodulin, occur during cardiopulmonary bypass (CPB). The possibility of a low anticoagulant potential when heparinization is reversed may be an important factor in the subsequent morbidity associated with thrombotic complications. Aprotinin is a serine protease inhibitor that in vitro binds competitively with the serine protease-activated protein C. However, aprotinin in the clinical setting has not been reported to alter levels of protein C in patients undergoing CPB. Reversal of the heparinization needed for CPB is almost universally performed with protamine. However, protamine has many deleterious effects. Recombinant platelet factor 4 (rPF4) has been proposed as an alternative to protamine. We investigated the effective heparin neutralization dose of rPF4 vs. the standard agent protamine in human blood activated through exposure to the CPB circuit. Activated clotting time (ACT) measurements suggested a 2:1 (w/w) reversal ratio for rPF4 and protamine. The first human open-label phase 1 trial of rPF4 reported no serious side effects and no important hemodynamic effects. Doses of 2.5 and 5.0 mg/kg were uniformly effective in reversing the anticoagulant effect of heparin and reducing the ACT to <200 s by 5 min after administration. Repeated monitoring of the ACT did not detect a rebound effect of heparin. RP DAmbra, M (reprint author), MASSACHUSETTS GEN HOSP,DEPT ANESTHESIA,CARDIAC ANESTHESIA GRP,14 FRUIT ST,BOSTON,MA 02114, USA. NR 40 TC 11 Z9 11 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0160-2446 J9 J CARDIOVASC PHARM JI J. Cardiovasc. Pharmacol. PY 1996 VL 27 SU 1 BP S58 EP S62 PG 5 WC Cardiac & Cardiovascular Systems; Pharmacology & Pharmacy SC Cardiovascular System & Cardiology; Pharmacology & Pharmacy GA TV464 UT WOS:A1996TV46400010 PM 8938285 ER PT J AU Yuan, JY AF Yuan, JY TI Evolutionary conservation of a genetic pathway of programmed cell death SO JOURNAL OF CELLULAR BIOCHEMISTRY LA English DT Article ID INTERLEUKIN-1-BETA CONVERTING ENZYME; NEMATODE CAENORHABDITIS-ELEGANS; BCL-2 GENE; C-ELEGANS; B-CELLS; APOPTOSIS; PROTEIN; EXPRESSION; SURVIVAL; CLONING AB Genetic analysis of programmed cell death in Caenorhabditis elegans has led to the identification of 13 genes that constitute a developmental pathway of programmed cell death. Two of the three key genes in this pathway, ced-9, a cell death suppressor, and ced-3, a cell death inducer, were found to encode proteins that share structural and functional similarities with the mammalian proto-oncogene product Bcl-2 and interleukin-1 beta converting enzyme, respectively. These results suggest that the genetic pathway of programmed cell death may be evolutionarily conserved from worms to mammals. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. RP Yuan, JY (reprint author), MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,149 13TH ST,4TH FLOOR,BOSTON,MA 02129, USA. NR 53 TC 67 Z9 86 U1 1 U2 8 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0730-2312 J9 J CELL BIOCHEM JI J. Cell. Biochem. PD JAN PY 1996 VL 60 IS 1 BP 4 EP 11 DI 10.1002/(SICI)1097-4644(19960101)60:1<4::AID-JCB2>3.0.CO;2-1 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA TT662 UT WOS:A1996TT66200002 PM 8825409 ER PT J AU Lo, EH Rogowska, J Bogorodzki, P Trocha, M Matsumoto, K Saffran, B Wolf, GL AF Lo, EH Rogowska, J Bogorodzki, P Trocha, M Matsumoto, K Saffran, B Wolf, GL TI Temporal correlation analysis of penumbral dynamics in focal cerebral ischemia SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article DE cerebral blood flow; dynamic computed tomography; functional imaging; ischemic penumbra ID BLOOD-FLOW; COMPUTED-TOMOGRAPHY; CEREBROVASCULAR-DISEASE; ARTERY OCCLUSION; BRAIN; THRESHOLDS; INJURY; HEMODYNAMICS; RATS; HYPOTHERMIA AB A novel temporal correlation technique was used to map the first-pass transit of iodinated contrast agents through the brain. Transit profiles after bolus injections were measured with dynamic computed tomography (CT) scanning (1 image/s over 50 s). A rabbit model of focal cerebral ischemia(n = 6) was used, and dynamic CT scans were performed at 30, 60, 90, and 120 min postocclusion. Within the ischemic core, no bolus transit was detectable, demonstrating that complete ischemia was present after arterial occlusion. In the periphery of the ischemic distribution, transit dynamics showed smaller peaks, broadened profiles, and overall delay in bolus transit. A cross-correlation method was used to generate maps of delays in ischemic transit profiles compared with normal transit profiles from the contralateral hemisphere. These maps showed that penumbral regions surrounding the ischemic core had significantly delayed bolus transit profiles. Enlargement of the ischemic core over time (from 30 to 120 min postocclusion) was primarily accomplished by the progressive deterioration of the penumbral regions. These results suggest that (a) temporal correlation methods can define regions of abnormal perfusion in focal cerebral ischemia, (b) peripheral regions of focal cerebral ischemia are characterized by delays in bolus transit profiles, and (c) these regions of bolus transit delay deteriorate over time and thus represent a hemodynamic penumbra. RP Lo, EH (reprint author), HARVARD MED SCH,CTR IMAGING & PHARMACEUT RES,DEPT RADIOL,MASSACHUSETTS GEN HOSP,MGH E BLDG 149,BOSTON,MA 02129, USA. NR 45 TC 27 Z9 33 U1 0 U2 2 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD JAN PY 1996 VL 16 IS 1 BP 60 EP 68 PG 9 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA TM214 UT WOS:A1996TM21400007 PM 8530556 ER PT J AU Matsumoto, K Lo, EH Pierce, AR Halpern, EF Newcomb, R AF Matsumoto, K Lo, EH Pierce, AR Halpern, EF Newcomb, R TI Secondary elevation of extracellular neurotransmitter amino acids in the reperfusion phase following focal cerebral ischemia SO JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM LA English DT Article DE gamma-aminobutyric acid; excitotoxicity; glutamate; microdialysis; reperfusion injury ID BLOOD-FLOW; RAT-BRAIN; ARTERY OCCLUSION; INTRACEREBRAL MICRODIALYSIS; ARACHIDONIC-ACID; GLOBAL-ISCHEMIA; TRANSIENT ISCHEMIA; FOREBRAIN ISCHEMIA; LIPID-PEROXIDATION; GERBIL BRAIN AB The purpose of this study was to evaluate amino acid neurotransmitter dynamics in the reperfusion phase after transient cerebral ischemia. In vivo microdialysis was used to measure extracellular amino acid levels in a rabbit model of focal ischemia. During 30 min of transient ischemia (n = 5), small but significant (p < 0.05) increases in glutamate, aspartate, gamma-aminobutyric acid (GABA), and taurine were noted. These elevations rapidly returned to baseline levels upon recirculation and remained constant for up to 5.5 h of reperfusion. In rabbits subjected to 2 h of transient ischemia (n = 5), two phases of amino acid release were seen. During ischemia, large (5- to 50-fold) elevations in glutamate, aspartate, GABA, and taurine occurred, as expected. These elevations rapidly normalized upon unocclusion. However, significant (p < 0.05) secondary elevations in glutamate, aspartate, and GABA occurred after 2-4 h of reperfusion. Regression analysis demonstrated significant correlations between primary (ischemic) and secondary (reperfusion) efflux. In permanent ischemia (n = 5), amino acid levels remained elevated throughout the entire experiment. Secondary elevations in excitatory amino acids may further contribute to the excitotoxic cascade during reperfusion. C1 HARVARD MED SCH,CTR IMAGING PHARMACEUT RES,MASSACHUSETTS GEN HOSP,BOSTON,MA 02129. NEUREX INC,MENLO PK,CA. KYOTO PREFECTURAL UNIV MED,DEPT NEUROSURG,KYOTO 602,JAPAN. FU NINDS NIH HHS [NS32806] NR 57 TC 84 Z9 86 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0271-678X J9 J CEREBR BLOOD F MET JI J. Cereb. Blood Flow Metab. PD JAN PY 1996 VL 16 IS 1 BP 114 EP 124 PG 11 WC Endocrinology & Metabolism; Hematology; Neurosciences SC Endocrinology & Metabolism; Hematology; Neurosciences & Neurology GA TM214 UT WOS:A1996TM21400014 PM 8530544 ER PT J AU Vamvakas, EC Pineda, AA AF Vamvakas, EC Pineda, AA TI Meta-analysis of clinical studies of the efficacy of granulocyte transfusions in the treatment of bacterial sepsis SO JOURNAL OF CLINICAL APHERESIS LA English DT Article DE granulocytes; leukocytes; neutrophils; transfusion; sepsis; septicemia; infection; granulocytopenia; leukopenia; neutropenia ID POLYMORPHONUCLEAR LEUKOCYTE TRANSFUSION; COLONY-STIMULATING FACTOR; BUFFY COAT TRANSFUSIONS; RANDOMIZED TRIAL; THERAPY; LEUKAPHERESIS; NEUTROPENIA; ASSOCIATION; SEPTICEMIA; INFECTIONS AB Background: Meta-analysis was used to explain disagreements across controlled clinical studies of the efficacy of granulocyte transfusions (GTX) in the treatment of bacterial sepsis. Methods: Studies published in English in 1970-1994 were retrieved. Seven studies of adults and five of neonates were eligible for analysis. Summary relative odds (RR) of survival in treated patients versus controls were computed for patient subsets defined on the basis of microbiologic proof of infection, recovery of bone marrow function, method of procurement of granulocytes for transfusion, dose of granulocytes transfused, assessment of leukocyte compatibility, and survival rate of controls. The random-effects method was used for all analyses. Results: Differences between the reviewed studies in the dose of granulocytes transfused and the survival rate of controls were primarily responsible for the disagreements across the reports. Adults (RR = 4.2) and neonates (RR = 18.0) receiving adequate doses of granulocytes and adults transfused in centers with a low survival rate of controls (RR = 8.9) experienced a significant (P < .05) benefit from GTX. Conclusion: GTX of adequate dose may be indicated in the 1990s for the treatment of sepsis in neonates, and perhaps also adults admitted to centers with an unusually high mortality rate of untransfused controls. More research is needed to reassess the proper role of GTX, in the light of modern transfusion medicine technology and the presently available options for the treatment of sepsis. (C) 1996 Wiley-Liss, Inc. C1 MAYO CLIN ROCHESTER,DIV TRANSFUS MED,ROCHESTER,MN. RP Vamvakas, EC (reprint author), MASSACHUSETTS GEN HOSP,BLOOD TRANSFUS SERV,GRJ-224,BOSTON,MA 02114, USA. NR 43 TC 61 Z9 62 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0733-2459 J9 J CLIN APHERESIS JI J. Clin. Apheresis PY 1996 VL 11 IS 1 BP 1 EP 9 PG 9 WC Hematology SC Hematology GA UH835 UT WOS:A1996UH83500001 PM 8722714 ER PT J AU Kuter, DJ AF Kuter, DJ TI Thrombopoietin: Biology, clinical applications, role in the donor setting SO JOURNAL OF CLINICAL APHERESIS LA English DT Article; Proceedings Paper CT 17th Annual Meeting of the American-Society-for-Apheresis CY MAR 21-23, 1996 CL SANTA FE, NM SP Amer Soc Apheresis DE platelets; megakaryocytes; hematopoiesis; cytokines; transfusion medicine; growth factors ID C-MPL LIGAND; PLATELET STORAGE LESION; RANDOMIZED PHASE-IB; IN-VITRO; MEGAKARYOCYTE GROWTH; THROMBOCYTOPENIC PURPURA; MEMBRANE-GLYCOPROTEINS; BONE-MARROW; MICE; ERYTHROPOIETIN AB Thrombopoietin (c-Mpl ligand) is the hematopoietic growth factor that is responsible for regulating the production of platelets from bone marrow megakaryocytes. This similar to 90 kd protein has recently been isolated and is comprised of an erythropoietin domain that is similar to 50% homologous to erythropoietin and a carbohydrate domain that is highly glycosylated and appears to stabilize the protein in the circulation. Thrombopoietin is produced in the liver and blood levels are determined by the mass of circulating platelets. However, there is no platelet ''sensor.'' Rather platelets contain high affinity thrombopoietin receptors that bind and remove thrombopoietin from the circulation and thereby directly determine circulating levels. In vitro thrombopoietin stimulates both early and late megakaryocyte precursors as well as some erythroid and multipotential progenitor cells. When administered to normal animals, it stimulates platelet production up to six-fold without affecting other lineages. However, when given to animals following chemotherapy or irradiation, it stimulates erythroid and myeloid as well as platelet recovery. Several different recombinant thrombopoietin proteins are now entering clinical trials in humans and all preliminary reports confirm a potent thrombopoietic stimulus and apparent lack of toxicity. Thrombopoietin shows great promise in preventing the thrombocytopenia associated with chemotherapy, bone marrow transplantation, and other acute or chronic thrombocytopenic disorders. In transfusion medicine, thrombopoietin may help mobilize peripheral blood progenitor cells, stimulate donors for plateletpheresis, and enhance platelet survival and function during storage. Many studies are currently underway in all these areas and should soon establish the role of thrombopoietin in clinical medicine. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,SCH MED,BOSTON,MA. RP Kuter, DJ (reprint author), MASSACHUSETTS GEN HOSP,HEMATOL ONCOL UNIT,COX 621,FRUIT ST,BOSTON,MA 02114, USA. FU NHLBI NIH HHS [HL54838] NR 69 TC 14 Z9 14 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0733-2459 J9 J CLIN APHERESIS JI J. Clin. Apheresis PY 1996 VL 11 IS 3 BP 149 EP 159 DI 10.1002/(SICI)1098-1101(1996)11:3<149::AID-JCA6>3.0.CO;2-B PG 11 WC Hematology SC Hematology GA VQ324 UT WOS:A1996VQ32400006 PM 8915820 ER PT J AU Gorlin, JB Humphreys, D Kent, P Galacki, D Kevy, SV Grupp, S Diller, L Weinstein, H Grier, H Shamberger, R AF Gorlin, JB Humphreys, D Kent, P Galacki, D Kevy, SV Grupp, S Diller, L Weinstein, H Grier, H Shamberger, R TI Pediatric large volume peripheral blood progenitor cell collections from patients under 25kg: A primer SO JOURNAL OF CLINICAL APHERESIS LA English DT Article DE peripheral blood stem cells; large volume leukopheresis; pediatric oncology ID STEM-CELLS; AUTOLOGOUS TRANSPLANTATION; DOSE CHEMOTHERAPY; BONE-MARROW; CHILDREN; EXPERIENCE; CANCER AB Collection of peripheral blood progenitor cells from small pediatric patients provides many social and technical challenges not faced when collecting from adult patients. This paper provides a single institutions experience with 85 collections from 14 patients less than 25 kg of weight over a 2 year period. Specific challenges include obtaining venous access, anticoagulation, volume shifts, and obtaining patient cooperation. A systematic analysis of options for access, alternative modes of anticoagulation, and the effect of large ratios of extra-corporeal volume to patient's blood volume are discussed. Access uniformly required central venous catheters (CVC) ranging from 7-10 Fr. Anticoagulation included systemic heparinization titrating dose by activated clotting time in all cases and combined with citrate at a ratio of 1:25-1:30 in most cases. Collections were performed on a COBE Spectra, after priming with leukoreduced irradiated red cells and omitting both the initial 120cc diversion and rinse back of red cells at the end. Social challenges include issues of assent and ability to distract patients for the duration of a prolonged collection. Progenitor yields from collections from 14 patients were quantitated by CD34+ assay in all cases and CFU-GM in ten of 14 patients. A median of 4.5 x 10(6)/kg CD34+ cells were obtained for each collection. Complications, including those related to catheter access, are enumerated. In summary, large volume peripheral blood progenitor collection can be safely and efficaciously performed in small pediatric patients. (C) 1996 Wiley-Liss, Inc. C1 CHILDRENS HOSP,TRANSFUS SERV,BOSTON,MA. CHILDRENS HOSP,BLOOD BANK,DEPT NURSING,APHERESIS UNIT,BOSTON,MA 02115. DANA FARBER CANC INST,BOSTON,MA 02115. CHILDRENS HOSP,DEPT SURG,BOSTON,MA. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. CHILDRENS HOSP PHILADELPHIA,PHILADELPHIA,PA 19104. NR 23 TC 42 Z9 43 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0733-2459 J9 J CLIN APHERESIS JI J. Clin. Apheresis PY 1996 VL 11 IS 4 BP 195 EP 203 DI 10.1002/(SICI)1098-1101(1996)11:4<195::AID-JCA4>3.0.CO;2-6 PG 9 WC Hematology SC Hematology GA WA207 UT WOS:A1996WA20700004 PM 8986865 ER PT J AU Samuels, MH Kramer, P AF Samuels, MH Kramer, P TI Differential effects of short-term fasting on pulsatile thyrotropin, gonadotropin, and alpha-subunit secretion in healthy men - A clinical research center study SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID LUTEINIZING-HORMONE SECRETION; FOLLICLE-STIMULATING-HORMONE; PITUITARY-TESTICULAR AXIS; TUMOR-NECROSIS-FACTOR; SERUM THYROTROPIN; RELEASE; AMPLITUDE; DOPAMINE; RAT AB In healthy subjects, short term fasting suppresses the hypothalamic-pituitary -thyroid and hypothalamic-pituitary-gonadal (HPG) axes, with decreased serum levels of TSH and LH. However, effects of fasting on pulsatile release of TSH, LH, FSH, and alpha-subunit are less clear. Eleven healthy young men each underwent two 2-day studies: a baseline study during normal caloric intake and a fasting study during 56 h of caloric deprivation. During the final 24 h of each study, blood samples were drawn every 15 min for measurement of serum TSH, LH, FSH, and a-subunit pulses. Fifty-six hours of fasting caused a 50% suppression of mean TSH levels and TSH pulse amplitude, without altering TSH pulse frequency. Nocturnal TSH pulse amplitude decreased by 60%, with abolition of the usual nocturnal TSH surge. Fasting suppressed mean LH levels and LH pulse amplitude by 30%, without affecting LH pulse frequency. In contrast, mean FSH levels only decreased by 13%, without changes in FSPI pulse parameters, whereas mean alpha-subunit levels and pulse amplitude decreased by 20%. These data show that short term fasting has a greater suppressive effect on the hypothalamic-pituitary-thyroid axis than on the HPG aids. Within the HPG axis, FSH is more resistant to fasting-induced suppression than LH, implying discordant regulation of the two gonadotropins during nutritional deprivation, alpha-Subunit suppression during fasting appears to parallel that seen for LH. C1 AUDIE L MURPHY MEM VET ADM MED CTR, SAN ANTONIO, TX 77030 USA. RP Samuels, MH (reprint author), OREGON HLTH SCI UNIV, DIV ENDOCRINOL, 3181 SW SAM JACKSON PK RD, PORTLAND, OR 97201 USA. FU NCRR NIH HHS [MO1-RR-00334, MO1-RR-00051] NR 25 TC 21 Z9 21 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD JAN PY 1996 VL 81 IS 1 BP 32 EP 36 DI 10.1210/jc.81.1.32 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TP939 UT WOS:A1996TP93900007 PM 8550771 ER PT J AU Miller, GM Alexander, JM Klibanski, A AF Miller, GM Alexander, JM Klibanski, A TI Gonadotropin-releasing hormone messenger RNA expression in gonadotroph tumors and normal human pituitary SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID ALPHA-SUBUNIT; LUTEINIZING-HORMONE; BETA-SUBUNIT; ANTERIOR-PITUITARY; GLYCOPROTEIN HORMONES; ADENOMAS INVIVO; SECRETION; THYROTROPIN; INVITRO; TRANSCRIPTION AB Gonadotroph tumors predominantly secrete FSH or free gonadotropin hormone subunits and rarely LH. In contrast to normal gonadotrophs, a subset of tumors synthesize FSH beta-subunit (SU) in excess of alpha-SU, and the cause of gonadotropin hormone-SU biosynthetic defects in these tumors is unknown. Gonadotropin-releasing hormone (GnRH) is known to modify gonadotropin hormone-SU biosynthesis and secretion and may be an important determinant of gonadotroph tumor hormone regulation. Data in experimental animals have demonstrated that endogenous expression of GnRH may occur in the pituitary. We therefore determined whether 1) the GnRH gene is expressed in gonadotroph tumors and normal pituitaries using reverse transcriptase (RT)-PCR; 2) the GnRH receptor gene is co-expressed in gonadotroph tumors; 3) an alternative upstream transcriptional start site on the GnRH gene is utilized; and 4) media from primary cultures of gonadotroph tumors have detectable GnRH immunoreactivity by RIA. GnRH messenger RNA (mRNA) was detected in 10/10 gonadotroph tumors, eight of which expressed GnRH-Receptor mRNA. Both mRNAs were detected in all normal pituitaries studied (n = 6). Six of 10 gonadotroph tumors and 316 normal pituitaries had GnRH transcripts derived from the upstream transcriptional start site (5'GnRH). GnRH immunoreactivity was detected in overnight media from 3/3 primary gonadotroph tumor cultures (range: 1.89-5.86pg/mL: limit of detection(LD) = < 1.58pg/mL) but was not detectable in control media. This is the first study to demonstrate endogenous GnRH gene expression in human pituitary adenomas and normal human pituitary tissue. The presence of both GnRH and GnRH-Rc suggest that GnRH may be a paracrine/autocrine regulator of cell function in the pituitary and may affect gonadotroph tumor hormone phenotype. C1 MASSACHUSETTS GEN HOSP, NEUROENDOCRINE UNIT, DEPT MED, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. FU NIDDK NIH HHS [DK07028, DK40947] NR 34 TC 31 Z9 31 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD JAN PY 1996 VL 81 IS 1 BP 80 EP 83 DI 10.1210/jc.81.1.80 PG 4 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TP939 UT WOS:A1996TP93900014 PM 8550798 ER PT J AU Baum, HBA Biller, BMK Katznelson, L Oppenheim, DS Clemmons, DR Cannistraro, KB Schoenfeld, DA Best, SA Klibanski, A AF Baum, HBA Biller, BMK Katznelson, L Oppenheim, DS Clemmons, DR Cannistraro, KB Schoenfeld, DA Best, SA Klibanski, A TI Assessment of growth hormone (GH) secretion in men with adult-onset GH deficiency compared with that in normal men - A clinical research center study SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID FACTOR-BINDING PROTEIN-2; RECOMBINANT HUMAN GH; FACTOR-I; PITUITARY-TUMORS; HUMAN-PLASMA; IGF-I; CHILDREN; AGE; RADIOIMMUNOASSAY; HYPOPITUITARISM AB It is not known how patients who acquire GH deficiency (GHD) in adulthood differ in measures of GH secretion from normal adults. To characterize measures of GH secretion in such patients compared to those in normal subjects, we studied 23 men (median age, 51 yr; range, 32-62 yr) with adult-onset pituitary disease, defined as GH-deficient based on having no detectable GH response to two pharmacological agents, and 17 normal men. Patients less than 50 yr old received insulin (0.1 U/kg, iv) and clonidine (0.15 mg, orally), whereas those 50 yr of age or older as well as normal controls received arginine (30 g, iv) and clonidine. Patients were compared to normal men by investigating GH sampling every 10 min for 24 h and serum levels of insulin-like growth factor I(IGF-I), IGF-binding protein 2 (IGFBP-2), IGFBP-3, and GH-binding protein. Frequent venous sampling of GH was analyzed in terms of mean 24-h levels, pooled 24-h GH, mean levels over the 12 h between 2000-0800 h (mean overnight GH level), and pulse analysis (pulses per 24 h and pulse amplitude) by the Pulsar computer program. Although there were significant differences between the two groups far almost all measures of GH secretion, overlap between the groups was always present. GH levels measured using a highly sensitive chemiluminescence assay on 24-h pools derived from frequent sampling displayed the least overlap between the two groups, as only 2 of 17 normal controls overlapped with the GHD patients. The pooled 24-h GPI level using this technique was significantly lower in patients with GHD than in controls (0.117 +/- 0.021 vs. 0.861 +/- 0.098 mu g/L; P < 0.0001). In the analysis of frequent GH sampling using a standard immunoradiometric assay, mean overnight GH levels provided the best separation between the two groups, as all 23 patients had values of 0.6 mu g/L or less, and 13 of 17 normal controls had values greater than 0.6 mu g/L. The mean overnight GH level in patients was 0.6 +/- 0.0 mu g/L compared to 1.0 +/- 0.1 mu g/L in controls (P < 0.0001). The mean 24-h GH level in patients was 0.5 +/- 0.0 mu g/L compared to 0.8 +/- 0.1 mu g/L in normal controls (P < 0.0001). GH pulse frequency and pulse amplitude were also reduced in patients with GHD compared to those in normal controls [1.7 +/- 0.5 vs. 5.1 +/- 0.5 pulses/24 h (P < 0.0001) and 0.6 +/- 0.1 us. 2.8 +/- 0.4 mu g/L (P < 0.0001), respectively]. The mean serum IGF-I level was significantly lower in patients with GHD than in normal controls (106.7 +/- 8.0 vs. 218.7 +/- 16.7 mu g/L; P < 0.0001). Three of 23 patients overlapped with control values. Mean serum levels of IGFBP-3 and the serum IGF-I/IGFBP-2 ratio were also significantly lower in patients than in controls, but values overlapped substantially. We conclude that overlap occurs on measures of GH secretion between normal men and men identified as GH deficient despite a stringent definition of GHD. The best separation was obtained using pooled 24-h GH levels determined by a highly sensitive chemiluminescence assay. C1 MASSACHUSETTS GEN HOSP, NEUROENDOCRINE UNIT, DEPT MED, BOSTON, MA 02114 USA. MASSACHUSETTS GEN HOSP, GEN CLIN RES CTR, BOSTON, MA 02114 USA. HARVARD UNIV, SCH MED, BOSTON, MA 02114 USA. MAINE MED CTR, DEPT MED, DIV ENDOCRINOL, PORTLAND, ME 04102 USA. UNIV N CAROLINA, DEPT MED, CHAPEL HILL, NC 27599 USA. FU NCRR NIH HHS [RR-01066]; NIDDK NIH HHS [DK-07028, DK-08783] NR 41 TC 78 Z9 78 U1 0 U2 0 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD JAN PY 1996 VL 81 IS 1 BP 84 EP 92 DI 10.1210/jc.81.1.84 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TP939 UT WOS:A1996TP93900015 PM 8550799 ER PT J AU Okuda, Y Adrogue, HJ Field, JB Nohara, H Yamashita, K AF Okuda, Y Adrogue, HJ Field, JB Nohara, H Yamashita, K TI Counterproductive effects of sodium bicarbonate in diabetic ketoacidosis SO JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM LA English DT Article ID CARNITINE PALMITOYLTRANSFERASE-I; KETONE-BODY PRODUCTION; PERFUSED-RAT-LIVER; ACID-BASE STATUS; MALONYL-COA; PH; KIDNEY; GLUCOSE; HOMEOSTASIS; METABOLISM AB Although a growing body of evidence supports that alkali therapy in diabetic ketoacidosis (DKA) might be counterproductive, our knowledge about the consequences of this treatment on ketone metabolism is limited. Consequently, we performed clinical and animal studies to further examine this topic. The clinical studies assessed seven patients with DKA treated with continuous insulin infusion at a low dosage. Three of them also received sodium bicarbonate (NaHCO3), whereas the remaining four acted as controls. The group receiving NaHCO3 showed a 6-h delay in the improvement of ketosis as compared with controls. In addition, there was an increase in acetoacetate (AcAc) levels during alkali administration, followed by an increase in 3-hydroxybutyrate (3-OHB) level after its completion. Significant differences were not found between groups in the response of plasma glucose to the overall therapy. The animal study examined the effects of a NaHCO3-rich perfusate on the hepatic production of ketones with the in situ rat-liver preparation. Alkali loading resulted in an immediate increase in the AcAc level followed by increases in both the 3-OHB level and the 3-OHB/AcAc ratio after its completion. Hepatic ketogenesis increased even further, to about twice the basal level, after termination of the NaHCO3 loading. This investigation confirms that alkali administration augments ketone production and unravels an effect of bicarbonate infusion that promotes a selective build up of AcAc in body fluids. The data support that alkali therapy in DKA has nonsaltuary effects in the metabolism and plasma levels of ketones. C1 DEPT VET AFFAIRS MED CTR, DEPT MED, RENAL SECT, HOUSTON, TX 77211 USA. BAYLOR COLL MED, HOUSTON, TX 77211 USA. ST LUKES EPISCOPAL HOSP, DIABET RES LAB, DIV ENDOCRINOL & METAB, HOUSTON, TX 77211 USA. NIIGATA UNIV, SCH DENT, DEPT BIOCHEM, NIIGATA, JAPAN. RP Okuda, Y (reprint author), UNIV TSUKUBA, INST CLIN MED, DEPT INTERNAL MED, 1-1-1 TENNODAI, TSUKUBA, IBARAKI 305, JAPAN. NR 40 TC 61 Z9 63 U1 0 U2 1 PU ENDOCRINE SOC PI CHEVY CHASE PA 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA SN 0021-972X EI 1945-7197 J9 J CLIN ENDOCR METAB JI J. Clin. Endocrinol. Metab. PD JAN PY 1996 VL 81 IS 1 BP 314 EP 320 DI 10.1210/jc.81.1.314 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA TP939 UT WOS:A1996TP93900050 PM 8550770 ER PT J AU Driever, W Fishman, MC AF Driever, W Fishman, MC TI The zebrafish: Heritable disorders in transparent embryos SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID GERM-LINE TRANSMISSION; FLOOR PLATE; CARDIOVASCULAR DEVELOPMENT; VERTEBRATE DEVELOPMENT; TRANSGENIC ZEBRAFISH; HEART TUBE; DROSOPHILA; MUTATIONS; NOTOCHORD; SYSTEM C1 MASSACHUSETTS GEN HOSP, CARDIOVASC RES CTR, CHARLESTOWN, MA 02199 USA. HARVARD UNIV, SCH MED, DEPT MED, CAMBRIDGE, MA 02115 USA. NR 63 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 2015 MANCHESTER RD, ANN ARBOR, MI 48104 USA SN 0021-9738 EI 1558-8238 J9 J CLIN INVEST JI J. Clin. Invest. PY 1996 VL 98 IS 11 SU S BP S41 EP S46 PG 6 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA VY385 UT WOS:A1996VY38500009 ER PT J AU Weiss, MJ Orkin, SH AF Weiss, MJ Orkin, SH TI In vitro differentiation of murine embryonic stem cells - New approaches to old problems SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID BONE MORPHOGENETIC PROTEIN-4; TRANSCRIPTION FACTOR GATA-1; MOUSE EMBRYOS; IN-VITRO; HEMATOPOIETIC DEVELOPMENT; ERYTHROID DEVELOPMENT; GENE-EXPRESSION; YOLK-SAC; ES-CELLS; PARAAORTIC SPLANCHNOPLEURA C1 CHILDRENS HOSP,HOWARD HUGHES MED INST,RES LABS,BOSTON,MA 02115. CHILDRENS HOSP,DANA FARBER CANC INST,DIV HEMATOL ONCOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. NR 60 TC 0 Z9 0 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PY 1996 VL 98 IS 11 SU S BP S13 EP S17 PG 5 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA VY385 UT WOS:A1996VY38500004 ER PT J AU Connolly, ES Winfree, CJ Springer, TA Naka, Y Liao, H Yan, SD Stern, DM Solomon, RA GutierrezRamos, JC Pinsky, DJ AF Connolly, ES Winfree, CJ Springer, TA Naka, Y Liao, H Yan, SD Stern, DM Solomon, RA GutierrezRamos, JC Pinsky, DJ TI Cerebral protection in homozygous null ICAM-1 mice after middle cerebral artery occlusion - Role of neutrophil adhesion in the pathogenesis of stroke SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article DE cerebral ischemia; stroke; ICAM-1 ID SYSTEM ISCHEMIC-INJURY; POLYMORPHONUCLEAR LEUKOCYTES; MONOCLONAL-ANTIBODY; REPERFUSION INJURY; BLOOD-FLOW; NO-REFLOW; MODEL; BRAIN; RAT; INFARCTION AB Acute neutrophil (PMN) recruitment to postischemic cardiac or pulmonary tissue has deleterious effects in the early reperfusion period, but the mechanisms and effects of neutrophil influx in the pathogenesis of evolving stroke remain controversial. To investigate whether PMNs contribute to adverse neurologic sequelae and mortality after stroke, and to study the potential role of the leukocyte adhesion molecule intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of stroke, we used a murine model of transient focal cerebral ischemia consisting of intraluminal middle cerebral artery occlusion for 45 min followed by 22 h of reperfusion, PMN accumulation, monitored by deposition of In-111-labeled PMNs in postischemic cerebral tissue, was increased 2.5-fold in the ipsilateral (infarcted) hemisphere compared with the contralateral (noninfarcted) hemisphere (P < 0.01). Mice immunodepleted of neutrophils before surgery demonstrated a 3.0-fold reduction in infarct volumes (P < 0.001), based on triphenyltetrazolium chloride staining of serial cerebral sections, improved ipsilateral cortical cerebral blood flow (measured by laser Doppler), and reduced neurological deficit compared with controls. In wild-type mice subjected to 45 min of ischemia followed by 22 h of reperfusion, ICAM-1 mRNA was increased in the ipsilateral hemisphere, with immunohistochemistry localizing increased ICAM-1 expression on cerebral microvascular endothelium, The role of ICAM-1 expression in stroke was investigated in homozygous null ICAM-1 mice (ICAM-1 -/-) in comparison with wild-type controls (ICAM-1 +/+). ICAM-1 -/- mice demonstrated a 3.7-fold reduction in infarct volume (P ( 0.005), a 35% increase in survival (P < 0.05), and reduced neurologic deficit compared with ICAM-1 +/+ controls, Cerebral blood flow to the infarcted hemisphere was 3.1-fold greater in ICAM-1 -/- mice compared with ICAM-1 +/+ controls (P < 0.01), suggesting an important role for ICAM-1 in the genesis of postischemic cerebral no-reflow, Because PMN-depleted and ICAM-1-deficient mice are relatively resistant to cerebral ischemia-reperfusion injury, these studies suggest an important role for ICAM-1-mediated PMN adhesion in the pathophysiology of evolving stroke. C1 COLUMBIA UNIV COLL PHYS & SURG,DEPT NEUROSURG,NEW YORK,NY 10032. COLUMBIA UNIV COLL PHYS & SURG,DEPT PHYSIOL,NEW YORK,NY 10032. COLUMBIA UNIV COLL PHYS & SURG,DEPT MED,NEW YORK,NY 10032. HARVARD UNIV,SCH MED,CTR BLOOD RES,BOSTON,MA 02138. FU NHLBI NIH HHS [HL42507, HL50629]; NICHD NIH HHS [HD13063] NR 59 TC 384 Z9 395 U1 1 U2 9 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 222 E 70TH STREET, NEW YORK, NY 10021 SN 0021-9738 J9 J CLIN INVEST JI J. Clin. Invest. PD JAN 1 PY 1996 VL 97 IS 1 BP 209 EP 216 DI 10.1172/JCI118392 PG 8 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA TQ105 UT WOS:A1996TQ10500029 PM 8550836 ER PT J AU Yandava, CN Zappulla, DC Korf, BR Neufeld, EJ AF Yandava, CN Zappulla, DC Korf, BR Neufeld, EJ TI ARMS test for diagnosis of factor V-Leiden mutation, a common cause of inherited thrombotic tendency SO JOURNAL OF CLINICAL LABORATORY ANALYSIS LA English DT Article DE activated protein C resistance; inherited thrombotic tendency; amplification refractory mutation system; polymerase chain reaction ID ACTIVATED PROTEIN-C; COAGULATION FACTOR-V; VENOUS THROMBOSIS; GENE MUTATION; RESISTANCE; THROMBOPHILIA; POPULATION; BLOOD; DNA AB We developed a simple and rapid amplification-refractory mutation system (ARMS) assay for the factor V mutation [R506Q] (factor V-Leiden), which results in the autosomal dominant thrombotic tendency, resistance to activated protein C (rAPC). PCR primers within Exon 10 of the factor V gene were designed. A common upstream primer was paired with either a mutant or wild-type-specific downstream primer. The 3'-most nucleotide of the specific primers recognized either the mutant or normal allele, and the 3' penultimate nucleotide was mismatched to enhance specificity of the reaction. The assay was validated using authentic factor V-Leiden DNA samples. Seven of 103 hematologically normal children (6.8%) were found to be heterozygotes. Among 27 patients studied by the rAPC assay, ARMS assay and rAPC results were concordant in 26. Among these were a 1-year-old child with a calcified clot in the inferior vena cava. Both the patient and his father were heterozygous for the mutation and both had abnormal rAPC assays. rAPC and factor V-Leiden assays were discordant in a young girl with a history of stroke. Biochemical rAPC assay was abnormal, while ARMS assay revealed amplification only with wild-type primers, suggesting a non-[R506Q] mechanism for rAPC. This assay will be a valuable tool for studying subjects with thromboses and their family members. (C) 1996 Wiley-Liss, Inc. C1 CHILDRENS HOSP,DIV HEMATOL ONCOL,BOSTON,MA 02115. CHILDRENS HOSP,DIV GENET,BOSTON,MA 02115. DANA FARBER CANC INST,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PEDIAT,BOSTON,MA 02115. RI Zappulla, David/C-7818-2009; Neufeld, Ellis/F-9331-2011; OI Zappulla, David/0000-0001-8242-3493 NR 19 TC 5 Z9 5 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0887-8013 J9 J CLIN LAB ANAL JI J. Clin. Lab. Anal. PY 1996 VL 10 IS 6 BP 414 EP 417 DI 10.1002/(SICI)1098-2825(1996)10:6<414::AID-JCLA17>3.0.CO;2-# PG 4 WC Medical Laboratory Technology SC Medical Laboratory Technology GA VV830 UT WOS:A1996VV83000017 PM 8951612 ER PT J AU Wexler, HM Molitoris, E Murray, PR Washington, J Zabransky, RJ Edelstein, PH Finegold, SM AF Wexler, HM Molitoris, E Murray, PR Washington, J Zabransky, RJ Edelstein, PH Finegold, SM TI Comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: A multilaboratory collaborative evaluation SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article AB A multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (SGE) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the MICs obtained by the SGE method with those obtained by the reference agar dilution method of the National Committee for Clinical Laboratory Standards. The standard deviation of the MIC obtained by the SGE method for the five participating laboratories was +/-0.26 of a twofold dilution, whereas it was +/-1 twofold dilution by the reference method. The interlaboratory reproducibility of the results for two control strains tested with imipenem, chloramphenicol, and metronidazole indicated that 96% of the measurements fell within +/-1 twofold dilution of the mode. The equivalence of the SGE method with the agar dilution method was assessed with a wide variety of anaerobic organisms. The MICs by both methods were within 1 doubling dilution in 93% of the measurements (n = 1,074). Discrepancies generally occurred with those organism-drug combinations that resulted in tailing endpoints (Fusobacterium nucleatum, 86% agreement) or in cases of light growth (Peptostreptococcus spp., 86% agreement). C1 W LOS ANGELES VET AFFAIRS MED CTR, MED SERV, LOS ANGELES, CA 90073 USA. W LOS ANGELES VET AFFAIRS MED CTR, RES SERV, LOS ANGELES, CA 90073 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MED, LOS ANGELES, CA 90024 USA. UNIV CALIF LOS ANGELES, SCH MED, DEPT MICROBIOL & IMMUNOL, LOS ANGELES, CA 90024 USA. WASHINGTON UNIV, SCH MED, DEPT PATHOL, ST LOUIS, MO 63110 USA. CLEVELAND CLIN FDN, DEPT CLIN PATHOL, CLEVELAND, OH 44195 USA. UNIV TEXAS, MED BRANCH, DEPT CLIN MICROBIOL & IMMUNOL, GALVESTON, TX 77550 USA. UNIV PENN, SCH MED, DEPT MED, PHILADELPHIA, PA 19104 USA. UNIV PENN, SCH MED, DEPT PATHOL & LAB MED, PHILADELPHIA, PA 19104 USA. NR 15 TC 16 Z9 16 U1 0 U2 0 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JAN PY 1996 VL 34 IS 1 BP 170 EP 174 PG 5 WC Microbiology SC Microbiology GA TL451 UT WOS:A1996TL45100035 PM 8748295 ER PT J AU Kirkwood, JM Strawderman, MH Ernstoff, MS Smith, TJ Borden, EC Blum, RH AF Kirkwood, JM Strawderman, MH Ernstoff, MS Smith, TJ Borden, EC Blum, RH TI Interferon alfa-2b adjuvant therapy of high-risk resected cutaneous melanoma: The Eastern Cooperative Oncology Group trial EST 1684 SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID MULTIFACTORIAL ANALYSIS; PROGNOSTIC FACTORS AB Purpose: Interferon alfa-2b (IFN(alpha-2b) exhibits antitumor activity in metastatic melanoma and on this basis has been evaluated as an adjuvant therapy following surgery for deep primary (T4) or regionally metastatic (N1) melanoma. Methods: A randomized controlled study of IFN alpha-2b (Schering-Plough, Kenilworth, NJ) administered at maximum-tolerated doses of 20 MU/m(2)/d intravenously (IV) for 1 month and 10 MU/m(2) three rimes per week subcutaneously (SC) for 48 weeks versus observation, was conducted by the Eastern Cooperative Oncology Group (ECOG) in 287 patients. Results: A significant prolongation of relapse-free survival(P =.0023, one-sided) and prolongation of overall survival (P =.0237, one-sided) was observed with IFN alpha-2b therapy in this trial, which is now mature with a median follow-up time of 6.9 years, The impact of treatment on relapse rate is most pronounced early during the treatment interval, The overall benefit of treatment in this trial was analyzed stratified by tumor burden and the presence or absence of microscopic nonpalpable and palpable regional lymph node metastasis, The benefit of therapy with IFN(alpha-2b was greatest among node-positive strata, Toxicity of IFN(alpha-2b required dose modification in the majority of patients, but treatment at greater than or equal to 80% of the scheduled dose was feasible in the majority of patients through the IV phase of treatment, and for more than 3 months of SC maintenance therapy, Discontinuation of treatment due to toxicity was infrequent after the fourth month of therapy. Conclusion: IFN alpha-2b prolongs the relapse-free interval and overall survival of high-risk resected melanoma patients. The increment in median disease free survival (from 1 to 1,7 years) and overall survival (from 2.8 to 3.8 years) that results from this therapy is associated with a 42% improvement in the fraction of patients who are continuously disease-free after treatment with IFN (from 26% to 37%) in comparison to observation, IFN alpha-2b is the first agent to show a significant benefit in relapse-free and overall survival of high-risk melanoma patients in a randomized controlled trial. (C) 1996 by American Society of Clinical Oncology. C1 DANA FARBER CANC INST,DIV BIOSTAT,BOSTON,MA. MORRISTOWN MEM HOSP,DEPT SURG,MORRISTOWN,NJ. UNIV MARYLAND,CTR CANC,BALTIMORE,MD 21201. NYU,MED CTR,DIV MED ONCOL,NEW YORK,NY. RP Kirkwood, JM (reprint author), UNIV PITTSBURGH,MED CTR,DIV MED ONCOL,200 LOTHROP ST,PITTSBURGH,PA 15213, USA. FU NCI NIH HHS [CA 18653, CA 21115, CA 23318] NR 13 TC 1319 Z9 1343 U1 1 U2 9 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD JAN PY 1996 VL 14 IS 1 BP 7 EP 17 PG 11 WC Oncology SC Oncology GA TP687 UT WOS:A1996TP68700003 PM 8558223 ER PT J AU Smith, M Arthur, D Camitta, B Carroll, AJ Crist, W Gaynon, P Gelber, R Heerema, N Korn, EL Link, M Murphy, S Pui, CH Pullen, J Reaman, G Sallan, SE Sather, H Shuster, J Simon, R Trigg, M Tubergen, D Uckun, F Ungerleider, R AF Smith, M Arthur, D Camitta, B Carroll, AJ Crist, W Gaynon, P Gelber, R Heerema, N Korn, EL Link, M Murphy, S Pui, CH Pullen, J Reaman, G Sallan, SE Sather, H Shuster, J Simon, R Trigg, M Tubergen, D Uckun, F Ungerleider, R TI Uniform approach to risk classification and treatment assignment for children with acute lymphoblastic leukemia SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID PEDIATRIC-ONCOLOGY-GROUP; UNFAVORABLE PRESENTING FEATURES; POOR-PROGNOSIS; CANCER-GROUP; LYMPHOMATOUS PRESENTATION; TREATMENT FAILURE; BLAST CELLS; CHILDHOOD; THERAPY; INTENSIFICATION AB Purpose: To define more uniform criteria for risk-based treatment assignment for children with acute lymphoblastic leukemia (ALL), the Cancer Therapy Evaluation Program (CTEP) of the National Cancer Institute (NCl) sponsored a workshop in September 1993. Participants included representatives from the Childrens Cancer Group (CCG), Pediatric Oncology Group (FOG), Dana-Farber Cancer Institute (DFCl), St Jude Children's Research Hospital (SJCRH), and the CTEP. Methods: Workshop participants presented and reviewed data from ALL clinical trials, using weighted averages to combine outcome data from different groups. Results: For patients with B-precursor (ie, non-T, non-B) ALL, the standard-risk category (4-year event-free survival [EFS] rate, similar to 80%) will include patients 1 to 9 years of age with a WBC count at diagnosis less than 50,000/mu L. The remaining patients will be classified as having high-risk ALL (4-year EFS rate, similar to 65%). For patients with T-cell ALL, different treatment strategies have yielded different conclusions concerning the prognostic significance of T-cell immunophenotype. Therefore, some groups/institutions will classify patients with T-cell ALL as high risk, while others will assign risk for patients with T-cell ALL based on the uniform age/WBC count criteria. Workshop participants agreed that the risk category of a patient may be modified by prognostic factors in addition to age and WBC count criteria, and that a common set of prognostic factors should be uniformly obtained, including DNA index (DI), cytogenetics, early response to treat ment (eg, day-14 bone marrow), immunophenotype, and CNS status. Conclusions: The more uniform approach to risk-based treatment assignment and to collection of specific prognostic factors should increase the efficiency of future ALL clinical research. C1 ST JUDE CHILDRENS RES HOSP, CHILDRENS CANC GRP, MEMPHIS, TN 38105 USA. ST JUDE CHILDRENS RES HOSP, PEDIAT ONCOL GRP, MEMPHIS, TN 38105 USA. DANA FARBER CANC INST, BOSTON, MA 02115 USA. RP Smith, M (reprint author), NCI, CANC THERAPY EVALUAT PROGRAM,CLIN INVEST BRANCH, PEDIAT SECT,ROOM 741, EXECUT PLAZA N, BETHESDA, MD 20892 USA. FU NCI NIH HHS [CA 13539, CA 29139, CA 30969] NR 42 TC 487 Z9 494 U1 1 U2 5 PU AMER SOC CLINICAL ONCOLOGY PI ALEXANDRIA PA 2318 MILL ROAD, STE 800, ALEXANDRIA, VA 22314 USA SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD JAN PY 1996 VL 14 IS 1 BP 18 EP 24 PG 7 WC Oncology SC Oncology GA TP687 UT WOS:A1996TP68700004 PM 8558195 ER PT J AU Tester, W Caplan, R Heaney, J Venner, P Whittington, R Byhardt, R True, L Shipley, W AF Tester, W Caplan, R Heaney, J Venner, P Whittington, R Byhardt, R True, L Shipley, W TI Neoadjuvant combined modality program with selective organ preservation for invasive bladder cancer: Results of Radiation Therapy Oncology Group phase II trial 8802 SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID TRANSITIONAL-CELL-CARCINOMA; SALVAGE CYSTECTOMY; CHEMOTHERAPY; CISPLATIN; METHOTREXATE; IRRADIATION; VINBLASTINE; SURGERY; RADIOTHERAPY; DOXORUBICIN AB Purpose: This phase II study was designed to evaluate effectiveness and toxicity of a combined chemoradiotherapy program with selective bladder preservation in the management of patients with invasive bladder cancer. Patients and Methods: Ninety-one eligible patients with invasive bladder cancer stages T2MO to T4AMO suitable for radical cystectomy received two courses of metholtrexate, cisplatin, and vinblastine (MCV regimen) followed by radiotherapy with 39.6 Gy and concurrent cisplatin. After complete urologic evaluation, operable patients who achieved complete response were selected for bladder preservation and treated with consolidation cisplatin-rodiotherapy. Results: Of 91 eligible patients, 85 underwent camplete urologic evaluation and 68 (75%; 95% confidence interval [CI], 59% to 84%) had documented complete responses. Fourteen operable patients with residual tumor underwent immediate cystectomy. Of 70 patients treated with consolidation cisplatin-radiotherapy, 36 subsequently developed bladder recurrences, 23 of which were invasive. patients with invasive recurrence (n = 16), extensive noninvasive recurrence (n = 6), or severe treatment complications (n = 1) underwent salvage cystectomy. Thus, a total of 37 of 91 patients (40%) required cystectomy. The 4 year cumulative risk of invasive local failure (which includes induction failures) was 43% (95% Cl, 33% to 53%). The 4-year actuarial risk of distant metastasis was 22% (95% Cl, 13% to 31%). The 4-year actuarial survival rate of the entire group was 62% (95% Cl, 52% to 72%). The 4-year actuarial rate of survival with bladder intact was 44% (95% Cl, 34% to 54%). Conclusion: Initial results of this combined chemoradiotherapy program show that bladder preservation can be achieved in the majority of patients, and that overall survival is similar to that reported with aggressive surgical approaches, Long-term survival and quality-of-life assessments require longer follow-up study. (C) 1996 by American Society of Clinical Oncology. C1 RADIAT THERAPY ONCOL GRP,PHILADELPHIA,PA 19141. HOSP UNIV PENN,PHILADELPHIA,PA 19104. FOX CHASE CANC CTR,PHILADELPHIA,PA 19104. DARTMOUTH HITCHCOCK MED CTR,LEBANON,NH. UNIV ALBERTA,CROSS CANC INST,EDMONTON,AB,CANADA. MED COLL WISCONSIN,MILWAUKEE,WI 53226. UNIV WASHINGTON,MED CTR,SEATTLE,WA 98195. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. RP Tester, W (reprint author), ALBERT EINSTEIN CANC CTR,5501 OLD YORK RD,WILLOWCREST BLDG,PHILADELPHIA,PA 19141, USA. NR 41 TC 175 Z9 181 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD JAN PY 1996 VL 14 IS 1 BP 119 EP 126 PG 8 WC Oncology SC Oncology GA TP687 UT WOS:A1996TP68700017 PM 8558186 ER PT J AU Minsky, BD Neuberg, D Kelsen, DP Pisansky, TM Ginsberg, R Benson, A AF Minsky, BD Neuberg, D Kelsen, DP Pisansky, TM Ginsberg, R Benson, A TI Neoadjuvant chemotherapy plus concurrent chemotherapy and high-dose radiation for squamous cell carcinoma of the esophagus: A preliminary analysis of the phase II intergroup trial 0122 SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Article ID THERAPY; RADIOTHERAPY; CISPLATIN; CANCER AB Purpose: To determine the preliminary acute toxicity and survival results of neoadjuvant chemotherapy followed by concurrent chemotherapy plus high-dose radiation therapy in patients with local/regional squamous cell carcinoma of the esophagus, Materials and Methods: Forty-five patients with clinical stage T1-4NO-1MO squamous cell carcinoma were entered onto the trial. Eight patients were declared ineligible after registration. Patients received three monthly cycles of fluorouracil (5-FU; 1,000 mg/m(2)/24 hr for 5 days) and cisplatin (100 mg/m(2) on day 1) (neoadjuvant segment) followed by two additional monthly cycles of 5-FU (1,000 mg/m(2)/24 hr for 5 days) and cisplatin (75 mg/m(2) on day 1) plus concurrent 64.8 Gy (combined modality segment). Results: With a median follow-up of 15 months in surviving patients, the incidence of total grade 3+ toxicity during the neoadjuvant chemotherapy segment was 61%, and during the combined modality segment was 72%. Of the 33 patients who started radiation therapy, 91% were able to complete the full course. There were six deaths during treatment, five of which (11%), because of nadir sepsis and/or dehydration, were treatment-related. For the 37 eligible patients, the median disease-free survival duration was 9 months, and the overall median survival was 20 months. Conclusion: The preliminary analysis of this trial demonstrated that the incidence of grade 3 + toxicity was similar to that reported in the combined modality arm of the prior Radiation Therapy Oncology Group (RTOG) intergroup esophageal trial RTOG 85-01. However, because of the increased incidence of treatment-related mortality, this treatment program will not be used as an experimental arm of intergroup trial INT 0123 (RTOG 94-05). (C) 1996 by American Society of Clinical Oncology. C1 MEM SLOAN KETTERING CANC CTR,DEPT MED,NEW YORK,NY 10021. MEM SLOAN KETTERING CANC CTR,DEPT SURG,NEW YORK,NY 10021. DANA FARBER CANC INST,DIV BIOSTAT,EASTERN COOPERAT ONCOL GRP,STAT OFF,BOSTON,MA 02115. MAYO CLIN & MAYO FDN,DIV RADIAT ONCOL,ROCHESTER,MN 55905. NORTHWESTERN UNIV,SCH MED,DEPT MED,DIV HEMATOL ONCOL,CHICAGO,IL 60611. RP Minsky, BD (reprint author), MEM SLOAN KETTERING CANC CTR,DEPT RADIAT ONCOL,275 YORK AVE,NEW YORK,NY 10021, USA. NR 9 TC 46 Z9 47 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD JAN PY 1996 VL 14 IS 1 BP 149 EP 155 PG 7 WC Oncology SC Oncology GA TP687 UT WOS:A1996TP68700021 PM 8558190 ER PT J AU Patterson, WB Emanuel, EJ AF Patterson, WB Emanuel, EJ TI Physician-drug company conflict of interest SO JOURNAL OF CLINICAL ONCOLOGY LA English DT Discussion ID OF-INTEREST C1 DANA FARBER CANC INST,BOSTON,MA 02115. NR 13 TC 1 Z9 1 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 SN 0732-183X J9 J CLIN ONCOL JI J. Clin. Oncol. PD JAN PY 1996 VL 14 IS 1 BP 316 EP 320 PG 5 WC Oncology SC Oncology GA TP687 UT WOS:A1996TP68700042 PM 8558213 ER PT J AU Schneider, LS Small, GW AF Schneider, LS Small, GW TI Clinical developments in Alzheimer's disease - Introduction SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Editorial Material C1 UNIV CALIF LOS ANGELES,INST NEUROPSYCHIAT,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,CTR AGING,LOS ANGELES,CA. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. RP Schneider, LS (reprint author), UNIV SO CALIF,SCH MED,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90089, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 14 BP 3 EP 4 PG 2 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA WE744 UT WOS:A1996WE74400001 ER PT J AU Peskind, ER AF Peskind, ER TI Neurobiology of Alzheimer's disease SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article; Proceedings Paper CT Symposium on Clinical Developments in Alzheimer's Disease at the Annual Meeting of the American-Association-for-Geriatric-Psychiatry (AAGP) CY FEB 14-17, 1996 CL TUCSON, AZ SP Amer Assoc Geriatr Psychiat ID AMYLOID-BETA-PROTEIN; LEWY BODY DEMENTIAS; PRECURSOR PROTEIN; SENILE DEMENTIA; NUCLEUS BASALIS; TAU-PHOSPHORYLATION; MISSENSE MUTATION; NEURONAL LOSS; GENE; LOCUS AB Although the specific process that destroys neurons in patients with Alzheimer's disease (AD) remains obscure, biochemical studies of AD neurohistologic lesions and molecular attempts to map and clone genes in familial AD have contributed greatly to our knowledge of AD. The major component of the extraneuronal neuritic plaque is beta-amyloid (A beta), which may be neurotoxic. The major component of the intraneuronal neurofibrillary tangle is hyperphosphorylated tau protein. It is unclear why this process damages the neuronal cytoskeleton. Familial AD is genetically heterogeneous. Chromosomes 21, 14, and 1 are causative genes in early-onset familial AD. The apolipoprotein E4 allele of chromosome 19 is a risk factor for both early- and late-onset AD. Unraveling the actions of these three causative genes and the apolipoprotein E4 allele may explain disease mechanisms common to all patients with AD. C1 UNIV WASHINGTON,SCH MED,DEPT PSYCHIAT & BEHAV SCI,SEATTLE,WA 98195. RP Peskind, ER (reprint author), VA PUGET SOUND HLTH CARE SYST,1660 COLUMBIAN WAY,SEATTLE,WA 98108, USA. NR 53 TC 5 Z9 6 U1 0 U2 0 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 14 BP 5 EP 8 PG 4 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA WE744 UT WOS:A1996WE74400002 PM 9024330 ER PT J AU Marder, SR AF Marder, SR TI Management of schizophrenia SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article; Proceedings Paper CT Symposium of the American-Psychiatric-Association on Psychotic Disorders - Many Forms, Common Themes CY MAY 21, 1995 CL MIAMI BEACH, FL SP Amer Psychiat Assoc ID CLINICAL-TRIAL; CLOZAPINE; THERAPY; DRUG; AFTERCARE; RELAPSE; FLUPHENAZINE; RISPERIDONE; HALOPERIDOL AB The Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition criteria for diagnosing schizophrenia require the presence of characteristic symptoms and disabilities, as well as the specific exclusion of other disorders with similar symptomatology. Once schizophrenia is diagnosed, the classification of schizophrenic symptoms as psychotic, disorganized, or negative can help clinicians predict treatment outcomes and individualize both pharmacologic and psychosocial treatment. Three treatment phases (acute, resolving, and maintenance) have been described; each has different medication strategies and goals. The introduction of novel antipsychotic agents, such as clozapine and risperidone, has enhanced the clinicians' ability to manage schizophrenic patients. Nonetheless, psychosocial treatment remains an important component of overall patient management. C1 UNIV CALIF LOS ANGELES,MED CTR,DEPT PSYCHIAT,LOS ANGELES,CA 90024. RP Marder, SR (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,PSYCHIAT SERV 116A,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 36 TC 18 Z9 18 U1 4 U2 5 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 3 BP 9 EP 13 PG 5 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA UP160 UT WOS:A1996UP16000003 PM 8626371 ER PT J AU Marder, SR AF Marder, SR TI Management of treatment-resistant patients with schizophrenia SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article ID DOUBLE-BLIND; ANTIPSYCHOTIC-DRUGS; NEGATIVE SYMPTOMS; CLINICAL-TRIAL; HALOPERIDOL; RISPERIDONE; CLOZAPINE; NEUROLEPTICS; MULTICENTER; MEDICATION AB This review will focus on three categories of poor response of patients with schizophrenia to an antipsychotic medication. The first category includes patients who continue to demonstrate positive psychotic symptoms when they receive adequate trials of an antipsychotic. These individuals may improve when their drug doses are altered or when they receive other drugs such as lithium or benzodiazepines in addition to their antipsychotic. Clozapine has been shown to result in substantial improvement in a majority of these patients. Additional evidence suggests that risperidone will also be effective in these individuals. The second category of poor responders consists of patients who are unable to tolerate the side effects of antipsychotics. These individuals may respond when they are changed to a newer antipsychotic. The third category includes patients who have persistent negative symptoms while they are treated with an antipsychotic. There is substantial evidence that these patients will demonstrate improvement in negative symptoms when they receive clozapine and risperidone as well as newer antipsychotics including olanzapine, sertindole, and quetiapine. C1 UNIV CALIF LOS ANGELES, SCH MED, DEPT PSYCHIAT & BIOBEHAV SCI, LOS ANGELES, CA 90024 USA. RP Marder, SR (reprint author), W LOS ANGELES VET AFFAIRS MED CTR, BRENTWOOD DIV, PSYCHIAT SERV 116A, 11301 WILSHIRE BLVD, LOS ANGELES, CA 90073 USA. NR 40 TC 25 Z9 28 U1 2 U2 2 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 USA SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 11 BP 26 EP 30 PG 5 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA VV133 UT WOS:A1996VV13300004 PM 8941168 ER PT J AU Baer, L AF Baer, L TI Behavior therapy: Endogenous serotonin therapy? SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article; Proceedings Paper CT Symposium on Anxiety Disorders - The Role of Serotonin/148th Annual Meeting of the American-Psychiatric-Association CY MAY, 1995 CL MIAMI BEACH, FL SP Amer Psychiat Assoc AB In numerous controlled trials, behavior therapy involving exposure and ritual prevention, either alone or in combination with pharmacotherapy, has been found to be highly effective in treating obsessive-compulsive disorder (OCD) and panic disorder. Although some animal research suggests that serotonin level affects classical conditioning, there is little knowledge of the effect of conditioning or reconditioning on serotonin. Indirect support for such an effect comes from the neuroimaging research finding that behavior therapy, as well as pharmacotherapy with a serotonin reuptake inhibitor, normalizes glucose metabolism in successfully treated OCD patients. A novel, automated approach to making behavior therapy more widely available via telephone is discussed. C1 HARVARD UNIV,SCH MED,DEPT PSYCHOL,CAMBRIDGE,MA 02138. RP Baer, L (reprint author), MASSACHUSETTS GEN HOSP,OCD CLIN,BOSTON,MA 02129, USA. NR 12 TC 16 Z9 16 U1 0 U2 0 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 6 BP 33 EP 35 PG 3 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA UU052 UT WOS:A1996UU05200006 PM 8647797 ER PT J AU Rosenbaum, JF Pollack, MH Pollock, RA AF Rosenbaum, JF Pollack, MH Pollock, RA TI Clinical issues in the long-term treatment of panic disorder SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article; Proceedings Paper CT Symposium on Use of SSRIs in Treating Panic Disorder CY AUG 11, 1995 CL BOSTON, MA ID COGNITIVE-BEHAVIOR THERAPY; FOLLOW-UP; ANXIETY SENSITIVITY; AGORAPHOBIA; ALPRAZOLAM; DISCONTINUATION; PREDICTORS; CHILDHOOD; EFFICACY AB Longitudinal studies in naturalistic settings have shed new light on the course and value of thera; peutic interventions in panic disorder and agoraphobia. Patients, their families, and clinicians need additional information about the natural course of the disorders and the factors that predispose patients to sustained illness, recovery, and relapse during treatment and upon treatment discontinuation. Clinical experience and controlled studies confirm the efficacy of pharmacologic and cognitive-behavioral therapy (CBT). Newer antidepressants, especially the serotonin selective reuptake inhibitors, represent a significant advance in effective pharmacologic intervention. However, despite the availability of effective treatment options, panic disorder often remains a chronic condition characterized by intermittent remissions and relapses over many years. Depression and comorbid anxiety disorders are associated with increased disease severity, treatment refractoriness, and relapse. The role of CBT as both a primary intervention and as an aid in the discontinuation of pharmacotherapy is reviewed. RP Rosenbaum, JF (reprint author), MASSACHUSETTS GEN HOSP,DEPT PSYCHIAT,CLIN PSYCHOPHARMACOL UNIT,15 PARKMAN ST,WAC 815,BOSTON,MA 02114, USA. RI Booker, Liesel/C-5282-2012 NR 25 TC 24 Z9 24 U1 1 U2 3 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 10 BP 44 EP 50 PG 7 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA VR896 UT WOS:A1996VR89600005 PM 8917131 ER PT J AU Marder, SR AF Marder, SR TI Clinical experience with risperidone SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article; Proceedings Paper CT Roundtable Meeting on Antipsychotic Drug Therapy - Current Status CY MAR 03, 1995 CL NEW ORLEANS, LA SP Janssen Pharm Inc ID NEUROLEPTIC MALIGNANT SYNDROME; CHRONIC-SCHIZOPHRENIC PATIENTS; OBSESSIVE-COMPULSIVE SYMPTOMS; DOUBLE-BLIND; CLOZAPINE WITHDRAWAL; ANTIPSYCHOTIC-DRUGS; PARKINSONS-DISEASE; TOURETTES-SYNDROME; HALOPERIDOL; DISORDER AB Recent clinical experiences with risperidone including controlled trials, clinical observations, and reports of side effects are reviewed. The controlled trials indicate that risperidone is an effective antipsychotic that may have advantages over conventional antipsychotics for treating both positive and negative symptoms of schizophrenia. A number of clinical reports indicate that risperidone is also effective for psychotic illnesses that result from other disorders. Risperidone has a relatively mild side effect profile when compared with conventional antipsychotics. C1 UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BEHAV SCI,LOS ANGELES,CA 90024. RP Marder, SR (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,BRENTWOOD DIV,PSYCHIAT SERV 116A,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 36 TC 16 Z9 16 U1 2 U2 4 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 9 BP 57 EP 61 PG 5 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA VK186 UT WOS:A1996VK18600010 PM 8823352 ER PT J AU Marmar, CR Schoenfeld, F Weiss, DS Metzler, T Zatzick, D Wu, R Smiga, S Tecott, L Neylan, T AF Marmar, CR Schoenfeld, F Weiss, DS Metzler, T Zatzick, D Wu, R Smiga, S Tecott, L Neylan, T TI Open trial of fluvoxamine treatment for combat-related posttraumatic stress disorder SO JOURNAL OF CLINICAL PSYCHIATRY LA English DT Article; Proceedings Paper CT Symposium on New Frontiers in OCD Spectrum Research for Psychiatry and Primary Care CY SEP 20-21, 1995 CL AUGUSTA, MI SP Solvay Pharm Inc, Upjohn Co ID DOUBLE-BLIND; PHENELZINE; IMIPRAMINE; PLACEBO; SEROTONIN; EVENTS AB A 10-week open-label trial of fluvoxamine was conducted for male Vietnam combat veterans with chronic PTSD. Subjects were excluded if they met full current criteria for panic disorder or agoraphobia, and lifetime criteria for psychosis, bipolar disorder, or organic mental syndrome. Repeated MANOVA was performed to determine change over time. Fluvoxamine was well tolerated; side effects were observed primarily early in treatment with headache, insomnia, sedation, and gastrointestinal distress being most frequent. Fluvoxamine was effective for treating the core intrusion, avoidance, and arousal symptoms of PTSD. Large treatment effects were seen by 4-6 weeks, and maintained at 10 weeks. The magnitude of change was greater than has been previously reported for antidepressant treatment of male Vietnam combat veterans with PTSD. C1 UNIV CALIF SAN FRANCISCO,DEPT PSYCHIAT,SAN FRANCISCO,CA 94143. US DEPT VET AFFAIRS,POSTTRAUMAT STRESS DISORDER PROGRAM,SAN FRANCISCO,CA. NR 26 TC 77 Z9 81 U1 1 U2 2 PU PHYSICIANS POSTGRADUATE PRESS PI MEMPHIS PA P O BOX 240008, MEMPHIS, TN 38124 SN 0160-6689 J9 J CLIN PSYCHIAT JI J. Clin. Psychiatry PY 1996 VL 57 SU 8 BP 66 EP 72 PG 7 WC Psychology, Clinical; Psychiatry SC Psychology; Psychiatry GA VC834 UT WOS:A1996VC83400011 PM 8698684 ER PT J AU Kosslyn, SM Thompson, WL Kim, IJ Rauch, SL Alpert, NM AF Kosslyn, SM Thompson, WL Kim, IJ Rauch, SL Alpert, NM TI Individual differences in cerebral blood flow in area 17 predict the time to evaluate visualized letters SO JOURNAL OF COGNITIVE NEUROSCIENCE LA English DT Article ID IMAGERY AB Sixteen subjects closed their eyes and visualized uppercase letters of the alphabet at two sizes, as small as possible or as large as possible while remaining ''visible.'' Subjects evaluated a shape characteristic of each letter (e.g., whether it has any curved lines), and responded as quickly as possible. Cerebral blood flow was normalized to the same value for each subject, and relative blood flow was computed for a set of regions of interest. The mean response time for each subject in the task was regressed onto the blood flow values. Blood flow in area 17 was negatively correlated with response time (r = -0.65), as was blood flow in area 19 (r = -0.66), whereas blood flow in the inferior parietal lobe was positively correlated with response time (r = 0.54). The first two effects persisted even when variance due to the other correlations was removed. These findings suggest that individual differences in the activation of specific brain loci are directly related to performance of tasks that rely on processing in those loci. C1 HARVARD UNIV,CAMBRIDGE,MA 02138. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. RI Park, Irene/D-1798-2010 NR 14 TC 63 Z9 63 U1 0 U2 1 PU MIT PRESS PI CAMBRIDGE PA 55 HAYWARD ST JOURNALS DEPT, CAMBRIDGE, MA 02142 SN 0898-929X J9 J COGNITIVE NEUROSCI JI J. Cogn. Neurosci. PD JAN PY 1996 VL 8 IS 1 BP 78 EP 82 DI 10.1162/jocn.1996.8.1.78 PG 5 WC Neurosciences; Psychology, Experimental SC Neurosciences & Neurology; Psychology GA UB459 UT WOS:A1996UB45900006 PM 23972237 ER PT J AU Bartlett, JD Xue, J Margolis, HC Moreno, EC AF Bartlett, JD Xue, J Margolis, HC Moreno, EC TI Identification of unique metalloproteinases from porcine enamel organ. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 67 EP 67 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100066 ER PT J AU Gao, XJ Fan, Y Margolis, HC Moreno, EC AF Gao, XJ Fan, Y Margolis, HC Moreno, EC TI Association of the composition of plaque fluid with caries activity SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 148 EP 148 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100150 ER PT J AU Margolis, HC Ruth, MB Moreno, EC Elliott, JC Anderson, P AF Margolis, HC Ruth, MB Moreno, EC Elliott, JC Anderson, P TI Solubility gradients in human enamel. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. LONDON HOSP,COLL MED,LONDON,ENGLAND. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 219 EP 219 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100218 ER PT J AU Esslinger, A Margolis, HC Moreno, EC AF Esslinger, A Margolis, HC Moreno, EC TI Applications of atomic force microscopy to dentin demineralization studies SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 221 EP 221 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100219 ER PT J AU Swartwout, S Niederman, R AF Swartwout, S Niederman, R TI Differentially expressed PMN genes regulated by propionic acid. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 255 EP 255 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100252 ER PT J AU Chapple, I Eftimiadi, C Dibart, S Socransky, S Matthews, J AF Chapple, I Eftimiadi, C Dibart, S Socransky, S Matthews, J TI Low molecular weight antioxidant activity in gingival crevicular fluid (GCF). SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. UNIV BIRMINGHAM,SCH DENT,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 256 EP 256 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100256 ER PT J AU Kapur, K Garrett, N Hamada, M Roumanas, E Freymiller, E Han, T Chen, T Levin, S AF Kapur, K Garrett, N Hamada, M Roumanas, E Freymiller, E Han, T Chen, T Levin, S TI Comparisons between insulin and non-insulin treated diabetic denture wearers. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH DENT,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 330 EP 330 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100333 ER PT J AU Garrett, WN Kapur, K Hamada, M Diener, R Freymiller, E Han, T Song, D Levin, S AF Garrett, WN Kapur, K Hamada, M Diener, R Freymiller, E Han, T Song, D Levin, S TI Functional comparisons between insulin and non-insulin treated diabetic denture wearers. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 331 EP 331 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100330 ER PT J AU Kapur, K Garrett, N Chen, T Jochen, D Song, D McFarland, R AF Kapur, K Garrett, N Chen, T Jochen, D Song, D McFarland, R TI Oral function comparisons between diabetic and non-diabetic denture wearers. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 UNIV CALIF LOS ANGELES,SCH DENT,LOS ANGELES,CA 90024. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 332 EP 332 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100334 ER PT J AU Lee, KH Weber, HP Maiden, MFJ Tanner, A AF Lee, KH Weber, HP Maiden, MFJ Tanner, A TI Microbiota of dental implants, crowned and natural teeth using DNA probes. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 HARVARD UNIV,SCH DENT MED,FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 498 EP 498 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100501 ER PT J AU Tavares, M Joshi, A Casthely, L AF Tavares, M Joshi, A Casthely, L TI A three year comparison of patient satisfaction with dental implants. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. HARVARD UNIV,SCH DENT MED,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 529 EP 529 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100529 ER PT J AU Lobene, R Hefferren, J AF Lobene, R Hefferren, J TI Clinical response on non-abrasive dentifrice use in dental hygienists. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. UNIV KANSAS,LAWRENCE,KS 66045. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 561 EP 561 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100561 ER PT J AU Lopman, J Maiden, MFJ Paster, BJ Dewhirst, F VanHoute, J AF Lopman, J Maiden, MFJ Paster, BJ Dewhirst, F VanHoute, J TI Identification acidogenesis, and 16S-rRNA-phylogeny of root surface non-mutans streptococci. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 619 EP 619 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100618 ER PT J AU Zhang, J Kashket, S Oda, D Niederman, R AF Zhang, J Kashket, S Oda, D Niederman, R TI Effects of short chain carboxylic acids on gingival epithelial cells. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. UNIV WASHINGTON,SEATTLE,WA 98195. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 680 EP 680 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100680 ER PT J AU Kania, AM Karimbux, NY Oh, S Nishimura, I AF Kania, AM Karimbux, NY Oh, S Nishimura, I TI Structural variation in the carboxyl end of rat collagen XII. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 HARVARD UNIV,SCH DENT MED,BOSTON,MA 02115. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 701 EP 701 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100699 ER PT J AU Eftimiadi, C Dibart, S Colombo, AP Socransky, SS AF Eftimiadi, C Dibart, S Colombo, AP Socransky, SS TI Increased leukotoxic effect elicited by phagocytosed A-actinomycetemcomitans compared with non-ingested bacteria SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 934 EP 934 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100935 ER PT J AU Cugini, MA Haffajee, AD Dibart, S Kent, RL Socransky, SS AF Cugini, MA Haffajee, AD Dibart, S Kent, RL Socransky, SS TI Biological effects of scaling and root planing .1. Clinical parameters. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 939 EP 939 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100938 ER PT J AU Haffajee, AD Dibart, S Cugini, MA Smith, C Kent, RL Socransky, SS AF Haffajee, AD Dibart, S Cugini, MA Smith, C Kent, RL Socransky, SS TI Biological effects of scaling and root planing .2. Microbiological changes. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 940 EP 940 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100942 ER PT J AU Socransky, SS Dibart, S Cugini, MA Smith, C Kent, RL Haffajee, AD AF Socransky, SS Dibart, S Cugini, MA Smith, C Kent, RL Haffajee, AD TI Biological effects of scaling and root planing .3. Poor responders. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 941 EP 941 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80100939 ER PT J AU Giannobile, WV Palys, M Howell, TH Haffajee, AD Socransky, SS AF Giannobile, WV Palys, M Howell, TH Haffajee, AD Socransky, SS TI Crevicular fluid ICTP in patients with periodontitis. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 HARVARD UNIV,SCH DENT MED,BOSTON,MA 02115. FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1114 EP 1114 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101117 ER PT J AU Dibart, S Eftimiadi, C Taubman, M Haffajee, AD Socransky, SS AF Dibart, S Eftimiadi, C Taubman, M Haffajee, AD Socransky, SS TI Evaluation of serum and crevicular fluid IgG subclass antibody to subgingival species using checkerboard immunoblotting. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1118 EP 1118 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101119 ER PT J AU Colombo, AP Haffajee, AD Dibart, S Eftimiadi, C Tanner, A Socransky, SS AF Colombo, AP Haffajee, AD Dibart, S Eftimiadi, C Tanner, A Socransky, SS TI IgG2 level and allotype, and Fc receptors in refractory periodontitis subjects. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1119 EP 1119 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101116 ER PT J AU Adriaens, PA Goodson, JM Encarnacion, M AF Adriaens, PA Goodson, JM Encarnacion, M TI Localized periodontal defects treated with subgingival scaling and root planing and actisite fibers. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 UNIV BRUSSELS,BRUSSELS,BELGIUM. FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1132 EP 1132 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101134 ER PT J AU Lingstrom, P Kashket, S VanHoute, J AF Lingstrom, P Kashket, S VanHoute, J TI The requirement for adaptation of Streptococcus mutans for Maltose fermentation. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1379 EP 1379 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101378 ER PT J AU Coleman, BE Tzellas, N Paster, BJ Dewhirst, FE AF Coleman, BE Tzellas, N Paster, BJ Dewhirst, FE TI Identification of 16S rRNA clones from a subject with ANUG as Atopobium species SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1515 EP 1515 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101513 ER PT J AU Paster, BJ Dewhirst, FE Shum, L Tanner, A Macuch, P AF Paster, BJ Dewhirst, FE Shum, L Tanner, A Macuch, P TI New Selenomonas species from diseased periodontal sites SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1516 EP 1516 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101517 ER PT J AU Paunovich, ED Cornell, JE Saunders, MJ AF Paunovich, ED Cornell, JE Saunders, MJ TI Oral health quality of life inventory: Influence of age and ethnicity SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,RCOHA,HOUSTON,TX. AUDIE L MURPHY MEM VET ADM MED CTR,CTR GERIATR RES EDUC & CLIN,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1776 EP 1776 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101774 ER PT J AU Macuch, PJ Tanner, A Maiden, MFJ Murray, L AF Macuch, PJ Tanner, A Maiden, MFJ Murray, L TI Cultivable microbiota of initial periodontal lesions SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1789 EP 1789 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101785 ER PT J AU Maiden, MFJ Tanner, A Macuch, PJ Murray, L AF Maiden, MFJ Tanner, A Macuch, PJ Murray, L TI DNA-probe analysis of initial periodontitis, gingivitis, & healthy microbiotas SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1790 EP 1790 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101789 ER PT J AU Kung, DS Nee, G Kaban, LB AF Kung, DS Nee, G Kaban, LB TI Comparitive analysis of open versus closed reduction of mandibular fractures SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,DEPT ORAL & MAXILLOFACIAL SURG,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1804 EP 1804 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101804 ER PT J AU Niederman, R WaraAswapati, N Masuno, K Socransky, S Uematsu, T Trong, D Genco, C Sandberg, E Beaudet, A AF Niederman, R WaraAswapati, N Masuno, K Socransky, S Uematsu, T Trong, D Genco, C Sandberg, E Beaudet, A TI Infection susceptibility in mice with ICAM-1 adhesion molecule deficiencies SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. BAYLOR COLL MED,BOSTON,MA. MOREHOUSE SCH MED,ATLANTA,GA 30310. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1815 EP 1815 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101810 ER PT J AU Sorkin, BC Oda, D Kandikonda, S Niederman, R AF Sorkin, BC Oda, D Kandikonda, S Niederman, R TI Growth regulation of human gingival epithelium SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. UNIV WASHINGTON,SEATTLE,WA 98195. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1816 EP 1816 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101814 ER PT J AU Yaskell, T Kashket, S Nelson, BJ AF Yaskell, T Kashket, S Nelson, BJ TI Effects of high bicarbonate in a dentifrice on intraoral demineralization SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. CHURCH & DWIGHT CO INC,PRINCETON,NJ. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 1885 EP 1885 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80101881 ER PT J AU Esch, TR Wong, ST Taubman, MA AF Esch, TR Wong, ST Taubman, MA TI Instruction of class II MHC on rat salivary gland cells SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,DEPT IMMUNOL,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2030 EP 2030 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102029 ER PT J AU Wucherpfennig, AL Li, YP Stashenko, P AF Wucherpfennig, AL Li, YP Stashenko, P TI Expression of cystatin C by human osteoclasts. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2325 EP 2325 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102320 ER PT J AU Smith, DJ Melvin, JL King, WF Pereira, MBB Taubman, MA AF Smith, DJ Melvin, JL King, WF Pereira, MBB Taubman, MA TI Effects of immunization with Streptococcus mutans glucan binding protein (GBP(59)) on infection with S-mutans. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,DEPT IMMUNOL,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2368 EP 2368 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102365 ER PT J AU Taubman, MA Eastcott, JW Smith, DJ DeWhirst, FE Esch, TR Holmberg, CJ AF Taubman, MA Eastcott, JW Smith, DJ DeWhirst, FE Esch, TR Holmberg, CJ TI CpG motifs in DNA enhance immune responses to systemic or mucosally administered tetanus toxoid. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,DEPT IMMUNOL,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2370 EP 2370 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102370 ER PT J AU Tzellas, N Paster, BJ Coleman, BE DeWhirst, FE AF Tzellas, N Paster, BJ Coleman, BE DeWhirst, FE TI Not yet cultivable spirochetes from ANUG sites. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2411 EP 2411 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102407 ER PT J AU Taubman, MA Smith, DJ Holmberg, CJ Eastcott, JW AF Taubman, MA Smith, DJ Holmberg, CJ Eastcott, JW TI Enhanced immunogenicity of caries relevant synthetic peptides. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,DEPT IMMUNOL,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2662 EP 2662 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102660 ER PT J AU Cielinski, MJ Kwong, Y Stashenko, P AF Cielinski, MJ Kwong, Y Stashenko, P TI Identification of gene products related to osteoclast recruitment and development. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2668 EP 2668 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102667 ER PT J AU Koutsoukos, J Bamgboye, P Ferrer, J Yip, J Boylan, R Craig, R Haffajee, A Socransky, S AF Koutsoukos, J Bamgboye, P Ferrer, J Yip, J Boylan, R Craig, R Haffajee, A Socransky, S TI Periodontal disease progression in urban minority populations. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 NYU,CD,NEW YORK,NY 10012. FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2689 EP 2689 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102686 ER PT J AU Yip, J Bucket, P Ferrer, J Koutsoukos, J Boylan, R Craig, R Haffajee, A Taubman, M AF Yip, J Bucket, P Ferrer, J Koutsoukos, J Boylan, R Craig, R Haffajee, A Taubman, M TI Serum IgG and periodontal disease progression in urban minority populations. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 NYU,CD,NEW YORK,NY. FORSYTH DENT CTR,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2690 EP 2690 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102690 ER PT J AU Bachiman, R Tavares, M Bivona, P Legeros, J Chung, CC Pei, Q AF Bachiman, R Tavares, M Bivona, P Legeros, J Chung, CC Pei, Q TI Caries prevalence of Pakistani parents and children in New York City. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 NYU,COLL DENT,NEW YORK,NY. FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2742 EP 2742 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102740 ER PT J AU Yelick, PC Law, CS Abduljabbar, T Stashenko, P AF Yelick, PC Law, CS Abduljabbar, T Stashenko, P TI TGF-beta family receptor expression during zebrafish craniofacial development. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2752 EP 2752 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102749 ER PT J AU Gatti, JJ Skobe, Z Dobeck, JM Smith, C Socransky, SS AF Gatti, JJ Skobe, Z Dobeck, JM Smith, C Socransky, SS TI Identification of bacteria in periapical lesions using DNA probes SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 HARVARD UNIV,SCH DENT MED,BOSTON,MA 02115. FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2849 EP 2849 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102846 ER PT J AU Stashenko, P Wang, CY AF Stashenko, P Wang, CY TI Effect of chronic apical periodontitis on pregnancy outcome in rats. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 FORSYTH DENT CTR,BOSTON,MA 02115. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2851 EP 2851 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102847 ER PT J AU Nara, Y Nakayama, K Nagakura, Y Ishizaka, C Dogon, IL AF Nara, Y Nakayama, K Nagakura, Y Ishizaka, C Dogon, IL TI Immediate bond strength of adhesive systems to sclerotic dentin measured by a portable tester. SO JOURNAL OF DENTAL RESEARCH LA English DT Meeting Abstract C1 NIPPON DENT UNIV TOKYO,TOKYO,JAPAN. HARVARD UNIV,SCH DENT MED,FORSYTH DENT CTR,CAMBRIDGE,MA 02138. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER ASSOC DENTAL RESEARCH PI ALEXANDRIA PA 1619 DUKE ST, ALEXANDRIA, VA 22314 SN 0022-0345 J9 J DENT RES JI J. Dent. Res. PY 1996 VL 75 SI SI BP 2943 EP 2943 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA TT801 UT WOS:A1996TT80102937 ER PT J AU Klein, RL Leong, GB Silva, JA AF Klein, RL Leong, GB Silva, JA TI Employee sabotage in the workplace: A biopsychosocial model SO JOURNAL OF FORENSIC SCIENCES LA English DT Article DE forensic science; psychiatry; forensic psychiatry; property damage; workplace; sabotage; aggression; violence ID BEHAVIOR; HUMOR; WORK AB Recently, there has been an increased interest in workplace violence. However, the psychiatric literature has paid little, if any attention to the specific subject area of workplace property harm or sabotage by employees. The specific psychology and/or psychopathology of the individual worker may be relevant in the evaluation of sabotage behavior. However, psychosocial factors associated with behavior within organizations and originating in part from the job itself are not likely to be considered in the initial assessment. We therefore introduce concepts from the organizational behavior literature that may facilitate and complement psychiatric evaluation of sabotage in the workplace. These concepts will fill the gap in a biopsychosocial assessment of sabotage in the workplace and provide a nexus for future interdisciplinary studies of workplace property violence. C1 UNIV CALIF IRVINE,GRAD SCH MANAGEMENT,IRVINE,CA 92717. UNIV MISSOURI,COLUMBIA,MO. UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,SAN ANTONIO,TX 78284. RP Klein, RL (reprint author), HARRY S TRUMAN MEM VET HOSP,PSYCHIAT SERV 116A,800 HOSP DR,COLUMBIA,MO 65201, USA. NR 18 TC 5 Z9 5 U1 1 U2 5 PU AMER SOC TESTING MATERIALS PI W CONSHOHOCKEN PA 100 BARR HARBOR DR, W CONSHOHOCKEN, PA 19428-2959 SN 0022-1198 J9 J FORENSIC SCI JI J. Forensic Sci. PD JAN PY 1996 VL 41 IS 1 BP 52 EP 55 PG 4 WC Medicine, Legal SC Legal Medicine GA UM893 UT WOS:A1996UM89300010 PM 8934699 ER PT J AU Kaufer, DI Cummings, JL Christine, D AF Kaufer, DI Cummings, JL Christine, D TI Effect of tacrine on behavioral symptoms in Alzheimer's disease: An open-label study SO JOURNAL OF GERIATRIC PSYCHIATRY AND NEUROLOGY LA English DT Article ID CHOLINERGIC HYPOTHESIS; SENILE DEMENTIA; DOUBLE-BLIND; TETRAHYDROAMINOACRIDINE; MULTICENTER; MEMORY AB We conducted an open-label study designed to assess the effects of tacrine on behavioral changes in patients with Alzheimer's disease (AD). Twenty-eight subjects completed a baseline evaluation and at least one assessment during treatment. Behavioral symptoms and cognitive function were assessed with the Neuropsychiatric Inventory (NPI) and Mini-Mental State Examination (MMSE), respectively. The mean NPI score at the maximum individual dose of tacrine attained was markedly decreased (behavior improved, compared to baseline). Symptoms of anxiety, apathy, hallucinations, aberrant motor behaviors, and disinhibition were most responsive. Subject stratification by dementia severity revealed a substantially reduced mean NPI score only in the group with moderate dementia, independent of cognitive response. Over half of the subjects with cognitive improvement had a marked reduction in behavioral symptoms, particularly apathetic behaviors. These data suggest that tacrine may be beneficial for selected behavioral symptoms in AD patients, particularly at higher doses and in those with moderate cognitive deficits. C1 W LOS ANGELES VET AFFAIRS MED CTR,BEHAV NEUROL SECT,PSYCHIAT SERV,NEUROBEHAV UNIT,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT NEUROL,LOS ANGELES,CA 90024. UNIV CALIF LOS ANGELES,SCH MED,DEPT PSYCHIAT & BIOBEHAV SCI,LOS ANGELES,CA 90024. NR 31 TC 176 Z9 179 U1 0 U2 0 PU DECKER PERIODICALS INC PI HAMILTON PA 4 HUGHSON STREET SOUTH PO BOX 620, LCD 1, HAMILTON ON L8N 3K7, CANADA SN 0891-9887 J9 J GERIATR PSYCH NEUR JI J. Geriatr. Psychiatry Neurol. PD JAN PY 1996 VL 9 IS 1 BP 1 EP 6 PG 6 WC Geriatrics & Gerontology; Clinical Neurology; Psychiatry SC Geriatrics & Gerontology; Neurosciences & Neurology; Psychiatry GA TY379 UT WOS:A1996TY37900001 PM 8679057 ER PT J AU Jenike, MA AF Jenike, MA TI Using an SSRI for post-herpetic neuralgia SO JOURNAL OF GERIATRIC PSYCHIATRY AND NEUROLOGY LA English DT Article ID DIABETIC NEUROPATHY; POSTHERPETIC NEURALGIA; AMITRIPTYLINE; RELIEVES; SYMPTOMS C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 6 TC 0 Z9 0 U1 0 U2 0 PU DECKER PERIODICALS INC PI HAMILTON PA 4 HUGHSON STREET SOUTH PO BOX 620, LCD 1, HAMILTON ON L8N 3K7, CANADA SN 0891-9887 J9 J GERIATR PSYCH NEUR JI J. Geriatr. Psychiatry Neurol. PD JAN PY 1996 VL 9 IS 1 BP 47 EP 47 PG 1 WC Geriatrics & Gerontology; Clinical Neurology; Psychiatry SC Geriatrics & Gerontology; Neurosciences & Neurology; Psychiatry GA TY379 UT WOS:A1996TY37900008 ER PT J AU Nahm, MH Siber, GR Olander, JV AF Nahm, MH Siber, GR Olander, JV TI A modified Farr assay is more specific than ELISA for measuring antibodies to Streptococcus pneumoniae capsular polysaccharides SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID CELL-WALL POLYSACCHARIDE; INFECTION; PHOSPHOCHOLINE; VACCINE; SERUM AB In an attempt to develop an assay specific for antibody to capsular polysaccharide (PS) of Streptococcus pneumoniae, the ability of ELISA and Farr (radioactive antigen-binding) assay for antibodies to 6B and 19A PS to be affected by antibodies to C-polysaccharide (C-PS) was compared. Preabsorption with C-PS reduced values obtained by ELISA for anti-6B antibody by >3-fold in 5 of 10 preimmune and 7 of 26 postimmune sera. In contrast, absorption reduced values by >3-fold in 0 of 36 samples studied with the Farr assay, Similar results were observed when the absorption was done with CSR-SCS2 S. pneumoniae. Furthermore, when anti-19A antibody levels were examined, preabsorption with R36a S. pneumoniae reduced ELISA values by 3-fold in 7 of 22 samples, whereas no samples had 3-fold reduction by Farr assay. Thus, the Farr assay for capsular PS is less affected than the ELISA by anti-C-PS antibody. C1 HARVARD UNIV,DANA FARBER CANC INST,SCH MED,DIV INFECT DIS,BOSTON,MA 02115. MASSACHUSETTS PUBL HLTH BIOL LABS,BOSTON,MA. RP Nahm, MH (reprint author), WASHINGTON UNIV,SCH MED,DIV LAB MED,BOX 8118,660 S EUCLID AVE,ST LOUIS,MO 63110, USA. OI Nahm, Moon/0000-0002-6922-1042 FU NIAID NIH HHS [AI-18125, AI-31473] NR 15 TC 19 Z9 19 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD JAN PY 1996 VL 173 IS 1 BP 113 EP 118 PG 6 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA TM343 UT WOS:A1996TM34300017 PM 8537647 ER PT J AU Chan, CY Molrine, DC George, S Tarbell, NJ Mauch, P Diller, L Shamberger, RC Phillips, NR Goorin, A Ambrosino, DM AF Chan, CY Molrine, DC George, S Tarbell, NJ Mauch, P Diller, L Shamberger, RC Phillips, NR Goorin, A Ambrosino, DM TI Pneumococcal conjugate vaccine primes for antibody responses to polysaccharide pneumococcal vaccine after treatment of Hodgkin's disease SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article ID CAPSULAR POLYSACCHARIDE; PROTEIN; INFECTIONS; INFANTS AB Thirty-nine previously treated Hodgkin's disease (HD) patients were immunized with 7-valent pneumococcal conjugate vaccine (7-OMPC) followed by one dose of 23-valent polysaccharide pneumococcal vaccine (23-PS). To determine the priming effect of 7-OMPC vaccine, their antibody responses to six serotypes contained in both vaccines were compared to those of 57 HD patients who received 23-PS vaccine only. The geometric mean antibody concentrations after immunization with 23-PS vaccine were significantly higher for five of the six measured serotypes in HD patients primed with 7-OMPC vaccine compared with responses in KD patients who received 23-PS vaccine only. The mean of the six antibody concentrations was significantly higher for the primed group at 12.5 mu g/mL and 7.76 mu g/mL, respectively (P = .015). Priming with a conjugate vaccine should be considered as a strategy to protect high-risk adults. C1 DANA FARBER CANC INST,JOINT CTR RADIAT THERAPY,INFECT DIS LAB,BOSTON,MA 02115. DANA FARBER CANC INST,JOINT CTR RADIAT THERAPY,DIV PEDIAT ONCOL,BOSTON,MA 02115. CHILDRENS HOSP,DEPT SURG,BOSTON,MA. FU NCI NIH HHS [CA-01730]; NIAID NIH HHS [AI-29623] NR 14 TC 81 Z9 83 U1 0 U2 0 PU UNIV CHICAGO PRESS PI CHICAGO PA 5720 S WOODLAWN AVE, CHICAGO, IL 60637 SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD JAN PY 1996 VL 173 IS 1 BP 256 EP 258 PG 3 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA TM343 UT WOS:A1996TM34300039 PM 8537671 ER PT J AU Sacca, R Kratz, A CamposNeto, A Hanson, MS Ruddle, NH AF Sacca, R Kratz, A CamposNeto, A Hanson, MS Ruddle, NH TI Lymphotoxin: From chronic inflammation to lymphoid organs SO JOURNAL OF INFLAMMATION LA English DT Article; Proceedings Paper CT 6th International Conference on TNF and Related Molecules CY MAY 08-12, 1996 CL RHODES, GREECE DE lymphotoxin; TNF-beta; autoimmune diseases; lymph node development ID CENTRAL-NERVOUS-SYSTEM; ANTIGEN-SPECIFIC LYMPHOCYTES; AUTOIMMUNE DEMYELINATION; UP-REGULATION; ADHESION; ENCEPHALOMYELITIS; VASCULATURE; MOLECULES C1 YALE UNIV,SCH MED,DEPT EPIDEMIOL & PUBL HLTH,NEW HAVEN,CT 06520. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. INFECT DIS RES INST,SEATTLE,WA. NR 14 TC 2 Z9 2 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 1078-7852 J9 J INFLAMM JI J. Inflamm. PY 1996 VL 47 IS 1-2 BP 81 EP 84 PG 4 WC Biochemistry & Molecular Biology; Cell Biology; Hematology; Immunology SC Biochemistry & Molecular Biology; Cell Biology; Hematology; Immunology GA VQ838 UT WOS:A1996VQ83800011 ER PT J AU Brent, GA Zavacki, AM AF Brent, GA Zavacki, AM TI Mutational analysis of the hormone response element in the human alcohol dehydrogenase gene defines features specific for retinoic acid and thyroid hormone response SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,ENDOCRINOL SECT,LOS ANGELES,CA 90073. HARVARD UNIV,SCH MED,PROGRAM BIOMED & BIOL SCI,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 1081-5589 J9 J INVEST MED JI J. Invest. Med. PD JAN PY 1996 VL 44 IS 1 BP A153 EP A153 PG 1 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA TP690 UT WOS:A1996TP69000818 ER PT J AU Lopez, A Moore, SA Richardson, A AF Lopez, A Moore, SA Richardson, A TI Regulation of adherence-stimulated hsp-70 mRNA expression in alveolar macrophages. SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Meeting Abstract C1 UNIV TEXAS,HLTH SCI CTR,SAN ANTONIO,TX. AUDIE L MURPHY MEM VET ADM MED CTR,GRECC,SAN ANTONIO,TX 78284. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 1081-5589 J9 J INVEST MED JI J. Invest. Med. PD JAN PY 1996 VL 44 IS 1 BP A38 EP A38 PG 1 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA TP690 UT WOS:A1996TP69000213 ER PT J AU Mericq, V Cassorla, F Salazar, T Avila, A Iniguez, G Bowers, CY Merriam, GR AF Mericq, V Cassorla, F Salazar, T Avila, A Iniguez, G Bowers, CY Merriam, GR TI Treatment with GHRP-2 accelerates the growth of GH deficient children SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Meeting Abstract C1 UNIV CHILE, INST INVEST MATERNO INFANTIL, SANTIAGO, 70118, CHILE. TULANE UNIV, SECT ENDOCRINOL & METAB, NEW ORLEANS, LA USA. VA PUGET SOUND HLTH CARE SYST, TACOMA, WA USA. VA PUGET SOUND HLTH CARE SYST, SEATTLE, WA USA. UNIV WASHINGTON, SCH MED, TACOMA, WA USA. UNIV WASHINGTON, SCH MED, SEATTLE, WA USA. RI Mericq, Veronica/F-3927-2010 NR 0 TC 2 Z9 2 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 1081-5589 J9 J INVEST MED JI J. Invest. Med. PD JAN PY 1996 VL 44 IS 1 BP A103 EP A103 PG 1 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA TP690 UT WOS:A1996TP69000549 ER PT J AU Ohta, K Pang, XP Berg, L Pekary, AE Hershman, JM AF Ohta, K Pang, XP Berg, L Pekary, AE Hershman, JM TI Anti-tumor actions of cytokines on new human papillary thyroid carcinoma cell lines SO JOURNAL OF INVESTIGATIVE MEDICINE LA English DT Meeting Abstract C1 W LOS ANGELES VET AFFAIRS MED CTR,ENDOCRINE RES LAB,LOS ANGELES,CA 90073. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 SN 1081-5589 J9 J INVEST MED JI J. Invest. Med. PD JAN PY 1996 VL 44 IS 1 BP A103 EP A103 PG 1 WC Medicine, General & Internal; Medicine, Research & Experimental SC General & Internal Medicine; Research & Experimental Medicine GA TP690 UT WOS:A1996TP69000553 ER PT J AU Fenton, MJ Kim, S Vermeulen, MW Alleva, D Kornfeld, H AF Fenton, MJ Kim, S Vermeulen, MW Alleva, D Kornfeld, H TI IFN-gamma produced by human macrophages infected with M.Tuberculosis may serve and auroregulatory role. SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Meeting Abstract C1 BOSTON UNIV,SCH MED,CTR PULM,BOSTON,MA 02118. MASSACHUSETTS GEN HOSP,PULM & CRIT CARE UNIT,CHARLESTOWN,MA 02129. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 SN 0741-5400 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PY 1996 SU S BP 94 EP 94 PG 1 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA VE906 UT WOS:A1996VE90600094 ER PT J AU Feigin, AM Takemoto, JY Wangspa, R Teeter, JH Brand, JG AF Feigin, AM Takemoto, JY Wangspa, R Teeter, JH Brand, JG TI Properties of voltage-gated ion channels formed by syringomycin E in planar lipid bilayers SO JOURNAL OF MEMBRANE BIOLOGY LA English DT Article DE syringomycin E; ion channels; voltage gating; lipid bilayers ID PSEUDOMONAS-SYRINGAE PHYTOTOXIN; DEPENDENT CHANNELS; PLASMA-MEMBRANE; SYRINGOTOXIN; CONDUCTANCE; MECHANISM AB Using the planar lipid bilayer technique we demonstrate that the lipodepsipeptide antibiotic, syringomycin E, forms voltage-sensitive ion channels of weak anion selectivity. The formation of channels in bilayers made from dioleoylglycerophosphatidylserine doped with syringomycin E at one side (1-40 mu g/ml) was greatly affected by cis-positive voltage. A change of voltage from a positive to a negative value resulted in (i) an abrupt increase in the single channel conductance (the rate of increase was voltage dependent) simultaneous with (ii) a closing of these channels and an exponential decrease in macroscopic conductance over time. The strong voltage dependence of multichannel steady state conductance, the single channel conductance, the rate of opening of channels at positive voltages and closing them at negative voltages, as well as the observed abrupt increase of single channel conductance after voltage sign reversal suggest that the change of the transmembrane field induces a significant rearrangement of syringomycin E channels, including a change in the spacing of charged groups that function as voltage sensors. The conductance induced by syringomycin E increased with the sixth power of syringomycin E concentration suggesting that at least six monomers are required for channel formation. C1 UTAH STATE UNIV,LOGAN,UT 84322. UNIV PENN,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA 19104. RP Feigin, AM (reprint author), MONELL CHEM SENSES CTR,PHILADELPHIA,PA 19104, USA. RI Takemoto, Jon/A-5309-2011 OI Takemoto, Jon/0000-0001-9919-9168 FU NIDCD NIH HHS [DC-00356, DC-01838] NR 24 TC 62 Z9 64 U1 0 U2 2 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0022-2631 J9 J MEMBRANE BIOL JI J. Membr. Biol. PD JAN PY 1996 VL 149 IS 1 BP 41 EP 47 DI 10.1007/s002329900005 PG 7 WC Biochemistry & Molecular Biology; Cell Biology; Physiology SC Biochemistry & Molecular Biology; Cell Biology; Physiology GA TQ969 UT WOS:A1996TQ96900005 PM 8825527 ER PT J AU Alterman, AI Snider, EC Cacciola, JS May, DJ Parikh, G Maany, I Rosenbaum, PR AF Alterman, AI Snider, EC Cacciola, JS May, DJ Parikh, G Maany, I Rosenbaum, PR TI A quasi-experimental comparison of the effectiveness of 6- versus 12-hour per week outpatient treatments for cocaine dependence SO JOURNAL OF NERVOUS AND MENTAL DISEASE LA English DT Article ID SEVERITY; ABUSERS C1 VET AFFAIRS MED CTR,PHILADELPHIA,PA. UNIV PENN,WHARTON SCH,DEPT STAT,PHILADELPHIA,PA 19104. RP Alterman, AI (reprint author), UNIV PENN,SCH MED,DEPT PSYCHIAT,TREATMENT RES CTR,3900 CHESTNUT ST,PHILADELPHIA,PA 19104, USA. RI Rosenbaum, Paul/H-8687-2012 FU NIDA NIH HHS [DA-05858, DA05186] NR 7 TC 9 Z9 9 U1 1 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3018 J9 J NERV MENT DIS JI J. Nerv. Ment. Dis. PD JAN PY 1996 VL 184 IS 1 BP 54 EP 56 DI 10.1097/00005053-199601000-00010 PG 3 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA TR860 UT WOS:A1996TR86000009 PM 8551291 ER PT J AU Peterfreund, RA MacCollin, M Gusella, J Fink, JS AF Peterfreund, RA MacCollin, M Gusella, J Fink, JS TI Characterization and expression of the human A2a adenosine receptor gene SO JOURNAL OF NEUROCHEMISTRY LA English DT Article DE adenosine receptor; gene structure; basal ganglia ID MOLECULAR-CLONING; HUMAN-CHROMOSOMES; HUMAN BRAIN; ENCODES AB The actions of the neurotransmitter adenosine are mediated by a family of high-affinity, G protein-coupled receptors. We have characterized the gene for the human A2a subtype of adenosine receptor (hA2aR) and determined levels of A2aR mRNA in human brain regions and nonneural tissues. Human genomic Southern blot analysis demonstrates the presence of a single gene encoding the hA2aR located on chromosome 22. Two overlapping cosmids containing the hA2aR gene were isolated from a chromosome 22 library and characterized, Southern blot and sequence analyses demonstrate that the hA2aR gene spans similar to 9-10 kb with a single intron interrupting the coding sequence between the regions encoding transmembrane domains III and IV. The sequence of the hA2aR gene diverged from the reported cDNA structure in the 5' untranslated region. This divergence appears to result from an artifact in the construction of the original cDNA library. By northern blot analysis, high expression of the hA2aR gene was identified in the caudate nucleus with low levels of expression in other brain regions. High expression was also seen in immune tissues; lesser A2aR expression was detected in heart and lung. The gene for the A2a subtype of receptor for the neurotransmitter adenosine falls in the class of intron containing G protein-coupled receptor genes. Depression in the basal ganglia is consistent with a role for the hA2aR in motor control. Activation of the A2aR may also regulate immune responses and cardiopulmonary function. C1 MASSACHUSETTS GEN HOSP,MOLEC NEUROGENET UNIT,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,MOLEC NEUROBIOL LAB,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT NEUROL,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. RP Peterfreund, RA (reprint author), MASSACHUSETTS GEN HOSP,DEPT ANESTHESIA,CLIN 3,FRUIT ST,BOSTON,MA 02114, USA. FU NHGRI NIH HHS [HG00317]; NIDA NIH HHS [DA07496]; NINDS NIH HHS [NS31579] NR 32 TC 60 Z9 60 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PD JAN PY 1996 VL 66 IS 1 BP 362 EP 368 PG 7 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA TK603 UT WOS:A1996TK60300045 PM 8522976 ER PT J AU Blass, JP Sheu, R Sarkar, P Wasco, W Tanzi, R AF Blass, JP Sheu, R Sarkar, P Wasco, W Tanzi, R TI Abnormal mitochondrial constituents coded by the nuclear genome in Alzheimer disease. SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 CORNELL UNIV MED COLL,BURKE MED RES INST,WHITE PLAINS,NY 10605. HARVARD UNIV,MASSACHUSETTS GEN HOSP,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1996 VL 66 SU 1 BP S73 EP S73 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA TY002 UT WOS:A1996TY00200292 ER PT J AU Dyer, CA Irizarry, MC Strickland, D Hyman, BT Shriver, EK AF Dyer, CA Irizarry, MC Strickland, D Hyman, BT Shriver, EK TI Apolipoprotein E receptor phenotype changes with astrocytic and oligodendrocytic differentiation of the 4C8 mouse glial cell line SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 EUNICE KENNEDY SHRIVER CTR MENTAL RETARDAT INC,WALTHAM,MA 02554. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. AMER RED CROSS,BETHESDA,MD 20855. NR 0 TC 0 Z9 0 U1 0 U2 1 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1996 VL 66 SU 1 BP S19 EP S19 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA TY002 UT WOS:A1996TY00200076 ER PT J AU Engleka, MJ Folli, F Winnay, J Kahn, CR McMorris, FA AF Engleka, MJ Folli, F Winnay, J Kahn, CR McMorris, FA TI Insulin-receptor substrate-1 is required for normal myelination SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104. JOSLIN DIABET CTR,DIV RES,BOSTON,MA 02215. NR 0 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1996 VL 66 SU 1 BP S20 EP S20 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA TY002 UT WOS:A1996TY00200077 ER PT J AU Sheu, KFR Sarkar, P Wasco, W Tanzi, R Blass, JP AF Sheu, KFR Sarkar, P Wasco, W Tanzi, R Blass, JP TI A gene locus of dihydrolipoyl succinyltransferase (DLST) is associated with Alzheimer's disease SO JOURNAL OF NEUROCHEMISTRY LA English DT Meeting Abstract C1 CORNELL UNIV,COLL MED,BURKE MED RES INST,WHITE PLAINS,NY 10605. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 3 Z9 3 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0022-3042 J9 J NEUROCHEM JI J. Neurochem. PY 1996 VL 66 SU 1 BP S10 EP S10 PG 1 WC Biochemistry & Molecular Biology; Neurosciences SC Biochemistry & Molecular Biology; Neurosciences & Neurology GA TY002 UT WOS:A1996TY00200039 ER PT J AU Dy, RC Kumar, R Scremin, OU AF Dy, RC Kumar, R Scremin, OU TI Clinical outcomes of dysphagia in elderly patients with cerebrovascular accident SO JOURNAL OF NEUROLOGIC REHABILITATION LA English DT Article DE cerebrovascular accident; dysphagia; videofluoroscopic study AB Dysphagia is a common complication for patients after cerebrovascular accident (CVA). The present study was done to determine the clinical outcome of dysphagia after CVA. The study also correlated the clinical outcome with the initial severity and location of the lesion. Method: A retrospective analysis of the videofluoroscopic study of patients who had clinical suspicion of dysphagia after stroke was performed. Forty-three patients met the inclusion criteria. All the patients included were 60 years of age or older with mean +/- SD of 70.09 +/- 8.69. The stroke diagnosis and location were documented by neuroimaging. Statistical analysis was done to determine whether initial severity of dysphagia, location of stroke, and age were predictive of dysphagia recovery. Results: The anatomical location of the lesion was predictive of dysphagia improvement. The highest incidence of improvement was noted in cortical stroke (65%), lowest in multiple strokes (12.5%), and intermediate values for subcortical (31%) and brainstem (20%) strokes (chi (2): 8.17; p < 0.0427). Initial severity of dysphagia and age was not predictive of improvement. There was a trend for increased risk of aspiration in patients with multi pie strokes, but this did not reach statistical significance. Conclusion: Improvement of dysphagia secondary to stroke is related to the anatomical location of the lesion, with better recovery in single cortical strokes and worse in multiple strokes. Improvement of dysphagia is not related to age or initial severity of dysphagia. C1 W Los Angeles Vet Affairs Med Ctr, Dept Phys Med & Rehabil, Los Angeles, CA 90073 USA. Univ Calif Los Angeles, Multicampus Fellowship Program Geriatr & Gerontol, Los Angeles, CA 90024 USA. Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90024 USA. W Los Angeles Vet Affairs Med Ctr, GRECC, Los Angeles, CA 90073 USA. Univ Calif Los Angeles, Sch Med, Dept Physiol, Los Angeles, CA 90024 USA. NR 16 TC 1 Z9 1 U1 0 U2 2 PU DEMOS MEDICAL PUBLISHING PI NEW YORK PA 386 PARK AVE SOUTH, STE 201, NEW YORK, NY 10016 USA SN 0888-4390 J9 J NEUROL REHABIL JI J. Neurol. Rehabil. PY 1996 VL 10 IS 4 BP 217 EP 222 PG 6 WC Clinical Neurology; Rehabilitation SC Neurosciences & Neurology; Rehabilitation GA V3055 UT WOS:000169571000002 ER PT J AU Alderson, L Fetell, MR Sisti, M Hochberg, F Cohen, M Louis, DN AF Alderson, L Fetell, MR Sisti, M Hochberg, F Cohen, M Louis, DN TI Sentinel lesions of primary CNS lymphoma SO JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY LA English DT Article DE primary CNS lymphoma; diagnosis; tumour regression; multiple sclerosis ID B-CELL LYMPHOMA; MULTIPLE-SCLEROSIS; BRAIN; TUMOR AB Some patients ultimately diagnosed with primary CNS lymphoma (PCNSL) have transient symptomatic contrast enhancing lesions. These ''sentinel lesions'' of PCNSL recede spontaneously or with corticosteroid treatment and present an important diagnostic dilemma because they show variable, but non-diagnostic histopathological features. Four previously healthy, immunocompetent patients aged 49 to 58 years had contrast enhancing intraparenchymal brain lesions. Before biopsy, three of the four were treated with corticosteroids. Initial biopsies showed demyelination with axonal sparing in two, non-specific inflammation in one, and normal brain in one. Infiltrating lymphocytes predominantly expressed T cell markers with rare B cells. All four patients recovered within two to four weeks after the initial biopsy and imaging studies showed resolution of the lesions. The CSF was normal in three of the four patients tested; oligoclonal bands were absent in both of the two tested. After seven to 11 months, each patient developed new symptomatic lesions in a different region of the brain, biopsy of which showed a B cell PCNSL. The mechanism of spontaneous involution of sentinal lesions is not understood, but may represent host immunity against the tumour. Sentinel lesions of PCNSL should be considered in patients with contrast enhancing focal parenchymal lesions that show non-specific or demyelinative histopathological changes. Close clinical and radiographic follow up is essential if PCNSL is to be diagnosed early in such patients. C1 COLUMBIA PRESBYTERIAN MED CTR,DEPT NEUROL,NEW YORK,NY. COLUMBIA PRESBYTERIAN MED CTR,DEPT NEUROSURG,NEW YORK,NY. MASSACHUSETTS GEN HOSP,NEUROL SERV,BOSTON,MA. MASSACHUSETTS GEN HOSP,NEUROSURG SERV,BOSTON,MA. MASSACHUSETTS GEN HOSP,DEPT PATHOL NEUROPATHOL,BOSTON,MA. HARVARD UNIV,SCH MED,BOSTON,MA. UNIV HOSP CLEVELAND,DEPT NEUROPATHOL,CLEVELAND,OH. NR 12 TC 82 Z9 85 U1 0 U2 0 PU BRITISH MED JOURNAL PUBL GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON, ENGLAND WC1H 9JR SN 0022-3050 J9 J NEUROL NEUROSUR PS JI J. Neurol. Neurosurg. Psychiatry PD JAN PY 1996 VL 60 IS 1 BP 102 EP 105 DI 10.1136/jnnp.60.1.102 PG 4 WC Clinical Neurology; Psychiatry; Surgery SC Neurosciences & Neurology; Psychiatry; Surgery GA TM739 UT WOS:A1996TM73900023 PM 8558135 ER PT J AU Litvan, I Hauw, JJ Bartko, JJ Lantos, PL Daniel, SE Horoupian, DS McKee, A Dickson, D Bancher, C Tabaton, M Jellinger, K Anderson, DW AF Litvan, I Hauw, JJ Bartko, JJ Lantos, PL Daniel, SE Horoupian, DS McKee, A Dickson, D Bancher, C Tabaton, M Jellinger, K Anderson, DW TI Validity and reliability of the preliminary NINDS neuropathologic criteria for progressive supranuclear palsy and related disorders SO JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY LA English DT Article DE neuropathology; Pick's disease; postencephalitic parkinsonism; progressive supranuclear palsy; reliability; validity ID ALZHEIMERS-DISEASE; NEUROFIBRILLARY TANGLES; POSTENCEPHALITIC PARKINSONISM; SENILE DEMENTIA; DEGENERATION; DIAGNOSIS AB We investigated the validity and reliability of diagnoses made by eight neuropathologists who used the preliminary NINDS neuropathologic diagnostic criteria for progressive supranuclear palsy (PSP) and related disorders. The specific disorders were typical, atypical, and combined PSP, postencephalitic parkinsonism, corticobasal ganglionic degeneration, and Pick's disease. These disorders were chosen because of the difficulties in their neuropathologic differentiation. We assessed validity by measuring sensitivity and positive predictive value. Reliability was evaluated by measuring pairwise and group agreement. From a total of 62 histologic cases, each neuropathologist independently classified 16 to 19 cases for the pairwise analysis and 5 to 6 cases for the group analysis. The neuropathologists were unaware of the study design, unfamiliar with the assigned cases, and initially had no clinical information about the cases. Our results showed that with routine sampling and staining methods, neuropathologic examination alone was not fully adequate for differentiating the disorders. The main difficulties were discriminating the subtypes of PSP and separating postencephalitic parkinsonism from PSP. Corticobasal ganglionic degeneration and Pick's disease were less difficult to distinguish from PSP. The addition of minimal clinical information contributed to the accuracy of the diagnosis. On the basis of results obtained, we propose clinicopathologic diagnostic criteria to improve on the NINDS criteria. C1 NINCDS,BIOMETRY & FIELD STUDIES BRANCH,BETHESDA,MD 20892. HOP LA PITIE SALPETRIERE,ASSOC CLAUDE BERNARD,INSERM U360,RAYMOND ESCOUROLLE NEUROPATHOL LAB,PARIS,FRANCE. NIMH,DIV EPIDEMIOL & RES STUDIES,BETHESDA,MD 20892. INST PSYCHIAT,DEPT NEUROPATHOL,LONDON SE5 8AF,ENGLAND. INST NEUROL,DEPT NEUROPATHOL,LONDON WC1N 3BG,ENGLAND. INST NEUROL,PARKINSONS DIS SOC BRAIN TISSUE BANK,LONDON WC1N 3BG,ENGLAND. STANFORD UNIV,SCH MED,DEPT PATHOL NEUROPATHOL,STANFORD,CA 94305. MASSACHUSETTS GEN HOSP,DEPT NEUROPATHOL,BOSTON,MA 02114. ALBERT EINSTEIN COLL MED,DEPT NEUROPATHOL,BRONX,NY. LAINZ HOSP,LUDWIG BOLTZMANN INST CLIN NEUROBIOL,A-1130 VIENNA,AUSTRIA. CASE WESTERN RESERVE UNIV,DIV NEUROPATHOL,CLEVELAND,OH 44106. RP Litvan, I (reprint author), NINCDS,NEUROEPIDEMIOL BRANCH,FED BLDG,ROOM 714,BETHESDA,MD 20892, USA. OI Dickson, Dennis W/0000-0001-7189-7917; Litvan, Irene/0000-0002-3485-3445 NR 39 TC 289 Z9 295 U1 0 U2 2 PU AMER ASSN NEUROPATHOLOGISTS INC PI LAWRENCE PA 1041 NEW HAMPSHIRE ST, LAWRENCE, KS 66044 USA SN 0022-3069 J9 J NEUROPATH EXP NEUR JI J. Neuropathol. Exp. Neurol. PD JAN PY 1996 VL 55 IS 1 BP 97 EP 105 DI 10.1097/00005072-199601000-00010 PG 9 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA TP445 UT WOS:A1996TP44500010 PM 8558176 ER PT J AU Peters, BN Masland, RH AF Peters, BN Masland, RH TI Responses to light of starburst amacrine cells SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID RETINAL GANGLION-CELLS; CENTRAL NERVOUS-SYSTEM; TIGER SALAMANDER RETINA; INNER PLEXIFORM LAYER; RABBIT RETINA; MAMMALIAN RETINA; BIPOLAR CELLS; SYNAPTIC TRANSMISSION; SLICE PREPARATION; RECEPTIVE-FIELDS AB 1. Starburst amacrine cells were studied using whole cell patch recording. Displaced starburst cells were labeled in rabbit retinas by intraocular injection of 4,6-diamidino-2-phenylindole. The retinas were isolated and maintained in vitro. The inner limiting membrane and Muller cell endfeet were removed mechanically from small areas above the starburst cell bodies, allowing an unimpeded approach under visual control to the cells. A total of 104 cells was studied. 2. In voltage-clamp recordings, the cells responded to light with slow, graded inward and outward currents on which were superimposed smaller, rapid inward currents. The rapid inward currents appeared to be postsynaptic currents. 3. The receptive fields of the cells were mapped using small spots. They had an on-center, off-surround organization. Visualizing the dendrites by including Lucifer yellow in the patch pipette showed that the receptive fields' centers closely approximated the dendritic spread of the neurons. 4. The cells' responses to movement were tested with smooth movements or with two-spot apparent motion. No directional preference was seen for spots swept across the whole receptive field, for centrifugal movements, or for centripetal movements. 5. Bath-applied tetrodotoxin (TTX) or intracellularly applied lidocaine N-ethyl bromide (QX-314) had no effect on any component of the spontaneous or light-evoked activity. Depolarization of the cell bodies by injected current showed evidence of active conductances, but they were unaffected by TTX or QX-314. 6. 6-Cyano-7-nitroquionxyline-2,3-dione eliminated the small rapid currents, indicating that they depend on alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/kainate glutamate receptors. 7. Because it is unlikely that we voltage clamped the distalmost dendrites of these wide-field cells, uncertainties remain about rapid electrical events occurring in the dendrites. From a functional point of view, though, the fact that slow responses to distal photic stimulation were recorded at the soma suggests that the starburst cells could in principle integrate inputs across fairly substantial fractions of their total dendritic arbors. The extent to which this actually occurs remains to be learned. C1 HARVARD UNIV,SCH MED,PROGRAM NEUROSCI,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,HOWARD HUGHES MED INST,BOSTON,MA 02114. FU NEI NIH HHS [EY-01075] NR 63 TC 63 Z9 63 U1 0 U2 1 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD JAN PY 1996 VL 75 IS 1 BP 469 EP 480 PG 12 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA TT526 UT WOS:A1996TT52600035 PM 8822571 ER PT J AU Parker, JA Wallis, JW Halama, JR Brown, CV Cradduck, TD Graham, MM Wu, E Wagenaar, DJ Mammone, GL Greenes, RA Holman, BL AF Parker, JA Wallis, JW Halama, JR Brown, CV Cradduck, TD Graham, MM Wu, E Wagenaar, DJ Mammone, GL Greenes, RA Holman, BL TI Collaboration using Internet for the development of case-based teaching files: Report of the computer and instrumentation council Internet focus group SO JOURNAL OF NUCLEAR MEDICINE LA English DT Article DE teaching files; Internet AB The Internet and particularly the World-Wide-Web is becoming a useful tool for the nuclear medicine community. Methods: The Computer and Instrumentation Council of the Society of Nuclear Medicine convened an Internet Focus group to discuss collaboration using the Internet. The prototype application considered was development of case-based teaching files using the World-Wide-Web. Teaching file cases (clinical history, images, description of findings and discussion) on World-Wide-Web servers at different institutions are integrated using the Internet. The user can navigate from case to case using point-and-click hypertext linking. Results: The initial experience with collaboration has been encouraging. An etiquette to help foster collaboration has been proposed. Development of quality control mechanisms and introduction of peer review were identified as issues needing further work. Conclusion: The World-Wide-Web offers great potential for new forms of collaboration. There is, however, a need to learn how to make best use of this new resource. C1 WASHINGTON UNIV,MALLINCKRODT INST RADIOL,ST LOUIS,MO. LOYOLA UNIV,MED CTR,MAYWOOD,IL 60153. W LOS ANGELES VET AFFAIRS MED CTR,LOS ANGELES,CA 90073. UNIV WESTERN ONTARIO,LONDON,ON,CANADA. WASHINGTON UNIV,SEATTLE,WA. FRANCIS A COUNTWAY LIB MED,BOSTON,MA. BRIGHAM & WOMENS HOSP,HARVARD MED SCH,BOSTON,MA 02115. RP Parker, JA (reprint author), BETH ISRAEL HOSP,DIV NUCL MED,HARVARD MED SCH,330 BROOKLINE AVE,BOSTON,MA 02215, USA. NR 27 TC 23 Z9 24 U1 0 U2 0 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 22090-5316 SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD JAN PY 1996 VL 37 IS 1 BP 178 EP 184 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TP052 UT WOS:A1996TP05200038 PM 8543991 ER PT J AU Sah, RL Trippel, SB Grodzinsky, AJ AF Sah, RL Trippel, SB Grodzinsky, AJ TI Differential effects of serum, insulin-like growth factor-I, and fibroblast growth factor-2 on the maintenance of cartilage physical properties during long-term culture SO JOURNAL OF ORTHOPAEDIC RESEARCH LA English DT Article ID BOVINE ARTICULAR-CARTILAGE; EXTRACELLULAR-MATRIX; STRESS-RELAXATION; CHONDROCYTES; PROTEOGLYCANS; COMPRESSION; EXPLANTS; ELECTROMECHANICS; METABOLISM; ALLOGRAFTS AB The effects of fetal bovine serum, insulin-like growth factor-I, and fibroblast growth factor-2 on the regulation of the functional physical properties of adult bovine cartilage explants during an incubation period of 18-20 days was determined, and the relationship between the measured functional properties of the cartilage and the tissue composition was assessed. Cartilage disks were tested in the uniaxial radially confined configuration by the application of low amplitude oscillatory displacement and measurement of the resultant load and streaming potential. For the control cartilage terminated just after explant, the modulus was 0.39 +/- 0.28 MPa, the open circuit hydraulic permeability was 2.0 +/- 1.0 x 10(-15) m(2)/(Pa . sec), and the electrokinetic (streaming potential) coefficient was -2.3 +/- 0.6 mV/MPa. Incubation of cartilage in medium supplemented with serum or insulin-like growth factor-I resulted in maintenance of the modulus and electrokinetic coefficient, whereas incubation in basal medium or medium supplemented with fibroblast growth factor-2 led to a marked decrease from control values in the modulus and the amplitude of the electrokinetic coefficient. All of the culture conditions examined resulted in an increase in permeability that was not statistically significant. The variation in the electromechanical properties of all the cartilage samples tested was related to the density of tissue proteoglycan and collagen (hydroxyproline). The modulus was correlated with both the density of tissue proteoglycan (+0.014 MPa/[mg/ml]) and the density of tissue hydroxyproline (+0.008 MPa/[mg/ml]). The electrokinetic coefficient was also correlated with the density of proteoglycan (-0.080 [mV/MPa]/[mg/ml]) and the density of hydroxyproline (+0.064 [mV/MPa]/[mg/ml]). These data indicate that the regulation of chondrocyte matrix metabolism by growth factors can significantly affect the physical properties and function of cartilage. C1 UNIV CALIF SAN DIEGO,INST BIOMED ENGN,LA JOLLA,CA 92093. MASSACHUSETTS GEN HOSP,ORTHOPAED RES LAB,BOSTON,MA 02114. MIT,CONTINUUM ELECTROMECH GRP,CAMBRIDGE,MA 02139. MIT,DEPT ELECT ENGN,CAMBRIDGE,MA 02139. MIT,DEPT MECH ENGN,CAMBRIDGE,MA 02139. HARVARD UNIV,MIT,DIV HLTH SCI & TECHNOL,CAMBRIDGE,MA 02139. RP Sah, RL (reprint author), UNIV CALIF SAN DIEGO,DEPT BIOENGN,9500 GILMAN DR,MAIL CODE 0412,LA JOLLA,CA 92093, USA. FU NIAMS NIH HHS [AR33236, AR31068] NR 55 TC 61 Z9 65 U1 0 U2 4 PU JOURNAL BONE JOINT SURGERY INC PI NEEDHAM PA 20 PICKERING ST, NEEDHAM, MA 02192 SN 0736-0266 J9 J ORTHOPAED RES JI J. Orthop. Res. PD JAN PY 1996 VL 14 IS 1 BP 44 EP 52 DI 10.1002/jor.1100140109 PG 9 WC Orthopedics SC Orthopedics GA UA588 UT WOS:A1996UA58800008 PM 8618165 ER PT J AU Caruso, EM Lewandrowski, KU Ohlendorf, C Tomford, WW Zaleske, DJ AF Caruso, EM Lewandrowski, KU Ohlendorf, C Tomford, WW Zaleske, DJ TI Repopulation of laser-perforated chondroepiphyseal matrix with xenogeneic chondrocytes: An experimental model SO JOURNAL OF ORTHOPAEDIC RESEARCH LA English DT Article ID MURINE MODEL; ARTICULAR SURFACES; TISSUE; REPLACEMENT; CARTILAGE; ABLATION; REPAIR; LIMBS AB Growth of chondrocytes into a xenogeneic chondroepiphyseal matrix was investigated in an in vitro experimental model by combining viable calf chondrocytes with chick epiphyseal matrix devoid of viable chondrocytes. The chondrocytes were harvested from the wrist joints of newborn calves and cultured for 2 days. The epiphyses were harvested from the distal femurs and the proximal tibias of fetal chicks after development was arrested at 17 days by freezing, The epiphyseal specimens were prepared in four ways. These included femoral and tibial epiphyses without holes and femoral and tibial epiphyses with holes made by a laser, These epiphyseal specimens were co-cultured with calf chondrocytes for various periods. After digestion of the epiphyseal matrix, viable chondrocytes were counted in suspension, Chondrocyte division in the matrix was assessed by [H-3]thymidine incorporation. The growth of calf chondrocytes into the xenogeneic chick matrix was evaluated by fluorescence microscopy on fresh thick epiphyseal sections, The percentage of viable chondrocytes in the xenogeneic epiphyseal matrix increased with culture time to a maximum al day 21. The addition of laser-drilled holes was found to extend a plateau of chondrocyte viability until day 29. A decrease in cell viability was detected at later observation points. This study demonstrates that xenogeneic matrix may serve as a morphogenetic scaffold for chondrocytic growth. C1 MASSACHUSETTS GEN HOSP,ORTHOPAED RES LABS,PEDIAT ORTHOPAED SERV,WACC 507,BOSTON,MA 02114. FU NIAMS NIH HHS [AR-39380] NR 22 TC 11 Z9 11 U1 0 U2 0 PU JOURNAL BONE JOINT SURGERY INC PI NEEDHAM PA 20 PICKERING ST, NEEDHAM, MA 02192 SN 0736-0266 J9 J ORTHOPAED RES JI J. Orthop. Res. PD JAN PY 1996 VL 14 IS 1 BP 102 EP 107 DI 10.1002/jor.1100140117 PG 6 WC Orthopedics SC Orthopedics GA UA588 UT WOS:A1996UA58800016 PM 8618151 ER PT J AU ODonnell, RJ Deutsch, TF Flotte, TJ Lorente, CA Tomford, WW Mankin, HJ Schomacker, KT AF ODonnell, RJ Deutsch, TF Flotte, TJ Lorente, CA Tomford, WW Mankin, HJ Schomacker, KT TI Effect of Er:YAG laser holes on osteoinduction in demineralized rat calvarial allografts SO JOURNAL OF ORTHOPAEDIC RESEARCH LA English DT Article ID BONE IMPLANTS; ABLATION; GEOMETRY; MATRIX; TUMORS AB Massive cortical autografts and allografts have been found to incorporate into host bone very slowly and thus are subject to complications such as fatigue fracture and infection. In order to understand and improve the process of osteogenesis in these types of bone grafts, a new experimental model was developed using bone discs from rat calvaria prepared by demineralization and drilling of 0.5 mm diameter holes with a pulsed, 2.94 mu m wavelength Erbium:Yttrium-Aluminum-Garnet laser. Four types of bone discs were analyzed: untreated (Type I): demineralized (Type II), laser-ablated (Type TIT), and laser-ablated then demineralized (Type IV). The discs were transplanted into a subcutaneous site in adult Sprague-Dawley rats and followed for as long as 6 weeks. Histologic analysis of the discs at weekly intervals with use of hematoxylin and eosin staining confirmed the presence of new bone growth in Type-II and Spe-IV discs. The amount of new bone growth in each disc was estimated by determining the mineral x-ray attenuation coefficient, which is proportional to mineral density, from digitized radiographs of the discs. The results showed that the processes of demineralization (p < 0.001) and laser ablation with demineralization (p < 0.05) were both significant in enhancing new bone growth in this model. This study demonstrated that osteoinduction can be fostered in cortical bone through the processes of demineralization and laser ablation. To the extent that laser ablation may allow maintenance of structural integrity while altering the surface geometry in such a way as to promote ingrowth of new bone, this experimental model represents an advance in understanding how osteogenesis in cortical bone grafts might be improved. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT ORAL & MAXILLOFACIAL SURG,BOSTON,MA 02114. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,ORTHOPAED SERV,BOSTON,MA 02114. FU NIAMS NIH HHS [AR-21896] NR 21 TC 17 Z9 17 U1 0 U2 3 PU JOURNAL BONE JOINT SURGERY INC PI NEEDHAM PA 20 PICKERING ST, NEEDHAM, MA 02192 SN 0736-0266 J9 J ORTHOPAED RES JI J. Orthop. Res. PD JAN PY 1996 VL 14 IS 1 BP 108 EP 113 DI 10.1002/jor.1100140118 PG 6 WC Orthopedics SC Orthopedics GA UA588 UT WOS:A1996UA58800017 PM 8618152 ER PT J AU McKee, MD Miranda, MA Riemer, BL Blasier, RB Redmond, BJ Sims, SH Waddell, JP Jupiter, JB AF McKee, MD Miranda, MA Riemer, BL Blasier, RB Redmond, BJ Sims, SH Waddell, JP Jupiter, JB TI Management of humeral nonunion after the failure of locking intramedullary nails SO JOURNAL OF ORTHOPAEDIC TRAUMA LA English DT Article DE humeral nonunion; interlocking nailing; humeral reconstruction ID SHAFT FRACTURES; SYSTEMS AB We reviewed 21 cases of humeral nonunion following the failure of ''locking'' humeral nails. The nails had been inserted as the primary operative procedure following humeral fracture in fifteen cases or after the failure of closed treatment in six cases. Reconstruction after the failure of these implants was complicated by poor bone stock and difficulty in achieving union. Although technically difficult, open reduction and internal fixation with plating and bone grafting (successful in nine of nine cases) was more consistent than exchange nailing (successful in four of 10 cases) in achieving union (p = 0.01). Two patients refused further surgical intervention. The degree of bone loss associated with a loose nail, the lack of success of exchange nailing, and the insertion site morbidity associated with humeral nail removal differentiate these nonunions from similar lower extremity problems. The degree of bone loss following failed locking nailing of the humerus is a major concern, and exchange nailing alone may not bean acceptable option to deal with this problem. C1 ST MICHAELS HOSP,UPPER EXTREM RECONSTRUCT SERV,TORONTO,ON M5B 1W8,CANADA. ALLEGHENY GEN HOSP,PITTSBURGH,PA 15212. UNIV TORONTO,DEPT SURG,DIV ORTHOPAED,TORONTO,ON,CANADA. MASSACHUSETTS GEN HOSP,ORTHOPAED TRAUMA SERV,BOSTON,MA 02114. NR 24 TC 65 Z9 71 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0890-5339 J9 J ORTHOP TRAUMA JI J. Orthop. Trauma PY 1996 VL 10 IS 7 BP 492 EP 499 DI 10.1097/00005131-199610000-00008 PG 8 WC Orthopedics; Sport Sciences SC Orthopedics; Sport Sciences GA VK560 UT WOS:A1996VK56000008 PM 8892150 ER PT J AU McKee, MD Pedlow, FX Cheney, PJ Schemitsch, EH AF McKee, MD Pedlow, FX Cheney, PJ Schemitsch, EH TI Fractures below the end of locking humeral nails: A report of three cases SO JOURNAL OF ORTHOPAEDIC TRAUMA LA English DT Article DE humeral shaft fracture; locking humeral nails; fracture below implant; complication ID PERITROCHANTERIC FRACTURES; DIAPHYSEAL FRACTURES; SHAFT FRACTURES AB We report three cases of fracture of the humerus that occurred at the tip of interlocking humeral nails inserted previously for humeral shaft fractures. The fractures occurred at 8, 10, and 26 weeks postoperatively, and all occurred through the distal interlocking screws after the patient sustained a rotational force to the arm. All three patients had further surgery, two subsequently healed their fractures, and one is still in active treatment. Orthopaedists should be aware of this complication when choosing interlocking humeral nails for the treatment of humeral shaft fractures. C1 ST MICHAELS HOSP,DEPT SURG,DIV ORTHOPAED,TORONTO,ON M5B 1W8,CANADA. MASSACHUSETTS GEN HOSP,ORTHOPAED TRAUMA SERV,BOSTON,MA 02114. HARVARD UNIV,BOSTON,MA 02115. EMERSON HOSP,CONCORD,MA. ST MICHAELS HOSP,UPPER EXTREM RECONSTRUCT SERV,TORONTO,ON M5B 1W8,CANADA. NR 27 TC 16 Z9 16 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 0890-5339 J9 J ORTHOP TRAUMA JI J. Orthop. Trauma PY 1996 VL 10 IS 7 BP 500 EP 504 DI 10.1097/00005131-199610000-00009 PG 5 WC Orthopedics; Sport Sciences SC Orthopedics; Sport Sciences GA VK560 UT WOS:A1996VK56000009 PM 8892151 ER PT J AU Clarke, EB French, B Bilodeau, ML Capasso, VC Edwards, A Empoliti, J AF Clarke, EB French, B Bilodeau, ML Capasso, VC Edwards, A Empoliti, J TI Management knowledge, attitudes and clinical practice: The impact of nurses' characteristics and education SO JOURNAL OF PAIN AND SYMPTOM MANAGEMENT LA English DT Article DE pain management; pain assessment; nurses' attitudes and knowledge regarding pain; nurses' characteristics; nurses' pain education ID PAIN MANAGEMENT; CANCER PAIN; ASSESSMENTS; INPATIENTS; MEDICATION; ADDICTION; DURATION AB This study examined the knowledge attitudes, and clinical practice of registered nurses (N = 120) regarding pain management. Data were collected from nine varied clinical units in a large, university-affiliated, teaching hospital in an urban area of the Northeast. Demographic information was also collected to explore the relationship between nurses' characteristics, including previous pain education, clinical experience, area of clinical practice, and other variables, and knowledge, attitudes, and clinical practice. Three instruments were wed in the study: (a) the Pain Management: Nurses' Knowledge and Attitude Survey; (b) a 12-item demographic questionnaire; and (c) a Pain Audit Tool (PAT) to gather data regarding pain assessment, documentation, and treatment practices from charts. Mean scores frp, the nursing knowledge and attitudes survey on pain revealed knowledge deficits and inconsistent responses in many areas related to pain management (mean, 62%; range, 41%-90%). The top two nurse-ranked barriers to pain management were related to patient reluctance to report pain and to take opioids for pain relief Demographic data revealed that education about pain was most inadequate in the following area: nonpharmacological interventions to relieve pain, the difference between acute and chronic pain, and the anatomy and physiology of pain. Chart audits with the Pain Audit Tool revealed that 76% of the charts (N = 82) lacked documentation of the we of a patient self-rating tool by nurses to assess pain, despite a high reported we (76%) of such a self-rating tool. Adjunct medications were ordered with some consistency but appeared to be underutilized. This was especially true of nonsteroidal anti-inflammatory agents (mean use, 1%). Ninety percent of the charts had no documentation of the we of nonpharmacological interventions to relieve pain. Although this clinical setting has policies and resources in place regarding the management of pain, it would appear that they are not optimal. Practical recommendations are presented for increasing nurses' knowledge about pain management; improving the quality and the consistency of the assessment, documentation and treatment of pain; and disseminating pain management information. C1 HARVARD UNIV,GRAD SCH EDUC,CAMBRIDGE,MA 02138. MASSACHUSETTS GEN HOSP,CTR PAIN,QUAL ASSURANCE RES & DEV SERV,BOSTON,MA. MASSACHUSETTS GEN HOSP,CTR PAIN,INTENS CARE NURSING SERV,BOSTON,MA. MASSACHUSETTS GEN HOSP,CTR PAIN,SURG NURSING SERV,BOSTON,MA. MASSACHUSETTS GEN HOSP,ORTHOPED NEUROSCI NURSING SERV,BOSTON,MA. NR 61 TC 128 Z9 132 U1 2 U2 9 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0885-3924 J9 J PAIN SYMPTOM MANAG JI J. Pain Symptom Manage. PD JAN PY 1996 VL 11 IS 1 BP 18 EP 31 DI 10.1016/0885-3924(95)00134-4 PG 14 WC Health Care Sciences & Services; Medicine, General & Internal; Clinical Neurology SC Health Care Sciences & Services; General & Internal Medicine; Neurosciences & Neurology GA TU366 UT WOS:A1996TU36600003 PM 8815146 ER PT J AU Standaert, DG Testa, CM Rudolf, GD Hollingsworth, ZR AF Standaert, DG Testa, CM Rudolf, GD Hollingsworth, ZR TI Inhibition of N-methyl-D-aspartate glutamate receptor subunit expression by antisense oligonucleotides reveals their role in striatal motor regulation SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID NMDA RECEPTOR; BASAL GANGLIA; 6-HYDROXYDOPAMINE MODEL; MOLECULAR DIVERSITY; PARKINSONS-DISEASE; BINDING-SITES; RAT; ACID; ANTAGONISTS; CHANNEL AB N-methyl-D-aspartate (NMDA) glutamate receptors have an established role in the regulation of motor behavior by the basal ganglia. Recent studies have revealed that NMDA receptors are heteromeric assemblies of structurally related subunits from two families: NMDAR1, which is required for channel activity, and NMDAR2A-D, which modulate the properties of the channels. In the rat, the NMDA receptor subunits exhibit anatomically restricted patterns of expression, so that each component of the basal ganglia has a distinct NMDA receptor subunit mRNA phenotype. We have used in vivo intrastriatal injection of synthetic antisense oligodeoxynucleotides (ODNs) to examine the roles of particular NMDA receptor subunits in the regulation of motor behavior in rats. Injection of 15 nmol of a 20-mer ODN targeted to the NMDAR1 subunit induced spontaneous ipsilateral rotation. Smaller doses of NMDAR1 antisense ODN did nor lead to spontaneous rotation, but prominent ipsilateral rotation was observed after systemic administration of D-amphetamine. An antisense ODN to NMDAR2A was also effective in eliciting amphetamine-inducible rotation, although the magnitude of the effect was less than that seen with NMDAR1, whereas ODNs targeted to NMDAR2B, NMDAR2C and an NMDAR1 sense strand ODN had no effect on behavior. In situ hybridization demonstrated that injection of the NMDAR1, NMDAR2A or NMDAR2B antisense ODNs produced specific reductions in target mRNA signal intensity in the injected striatum. After NMDAR1 antisense ODN injection, striatal binding of H-3-glutamate to NMDA sites was not altered, although strychnine-insensitive H-3-glycine binding sites exhibited a small but significant reduction. These observations suggest that NMDA receptor complexes containing NMDAR1 and, to a lesser extent, NMDAR2A subunits play particularly important roles in the regulation of motor behavior by neostriatal neurons. RP Standaert, DG (reprint author), MASSACHUSETTS GEN HOSP,DEPT NEUROL,WARREN 408,FRUIT ST,BOSTON,MA 02114, USA. OI Standaert, David/0000-0003-2921-8348 NR 54 TC 38 Z9 40 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD JAN PY 1996 VL 276 IS 1 BP 342 EP 352 PG 11 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA TP439 UT WOS:A1996TP43900046 PM 8558453 ER PT J AU DieTrill, M Bromberg, J LaVally, B Portales, LA SanFeliz, A Patenaude, AF AF DieTrill, M Bromberg, J LaVally, B Portales, LA SanFeliz, A Patenaude, AF TI Development of social skills in boys with brain tumors: A group approach SO JOURNAL OF PSYCHOSOCIAL ONCOLOGY LA English DT Article ID NEWLY-DIAGNOSED CANCER; CHILDHOOD-CANCER; HEAD-INJURY; CHILDREN; ADJUSTMENT; SURVIVORS; QUALITY; LIFE; MEDULLOBLASTOMA; CHEMOTHERAPY AB Children with brain tumors often experience organically based illness- and treatment-related sequelae that interfere with the development of complex social skills necessary for confident, mutually satisfying interactions with peers. This article describes a structured, time-limited group focused on the development of social skills in boys who have a brain tumor. Social support and medical education were additional components. A parallel group for parents focused on relevant issues that arose in the boys' group and on the parents' general concerns about their child's social integration. The uniqueness of this program stemmed from the combination of development of social skills, specificity and homogeneity of the target population, highly structured group interventions, and parallel parent group. The program's efficacy in developing specific social skills was reported by both the boys and their parents. The authors recommend offering similar group interventions to other medically ill children and their parents, extending the model to female pediatric patients with brain tumors, and comparing time-limited versus ongoing groups for children with brain tumors. C1 DANA FARBER CANC INST,DIV PEDIAT ONCOL,BOSTON,MA 02115. WHEELOCK COLL,DEPT HUMAN DEV,BOSTON,MA. DANA FARBER CANC INST,DIV ONCOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PSYCHIAT,BOSTON,MA 02115. RP DieTrill, M (reprint author), HOSP GEN UNIV GREGORIO MARANON,DEPT ONCOL,DOCTOR ESQUERDO 46,MADRID 28007,SPAIN. NR 52 TC 6 Z9 6 U1 5 U2 5 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0734-7332 J9 J PSYCHOSOC ONCOL JI J. Psychosoc. Oncol. PY 1996 VL 14 IS 2 BP 23 EP 41 DI 10.1300/J077V14N02_02 PG 19 WC Psychology, Social SC Psychology GA VX944 UT WOS:A1996VX94400002 ER PT J AU Smith, RM Zhang, SL White, MF Jarett, L AF Smith, RM Zhang, SL White, MF Jarett, L TI The role of receptor kinase activity and the NPEY(960) motif in insulin-accelerated receptor-mediated insulin internalization SO JOURNAL OF RECEPTOR AND SIGNAL TRANSDUCTION RESEARCH LA English DT Article ID FLUID-PHASE ENDOCYTOSIS; JUXTAMEMBRANE REGION; HEPATOMA-CELLS; BINDING; ACCUMULATION; PATHWAY AB This study used biochemical and quantitative ultrastructural approaches to examine the roles that insulin receptor beta subunit kinase activity, the NPEY motif in the juxtamembrane region, and tyrosine phosphorylation within that domain plays in insulin-accelerated receptor-mediated insulin internalization in CHO cells. Internalization of insulin in cells that expressed kinase-deficient receptors (CHOA1018) Or receptors lacking the NPEY Ala(954)-Asp(965) domain (CHODelta 960) was reduced by 80% compared to cells expressing wild-type human insulin receptors (CHOHIRc) Ultrastructural analysis revealed that the decreased internalization in CHOA1018 cells was due to the reduced ability of the kinase deficient receptor to migrate from the microvilli of cultured cells and aggregate on the cell surface. Deletion of the NPEY motif in the juxtamembrane region of the beta subunit severely reduced receptor migration, interfered with the normal aggregation of receptors on the cell surface, and virtually eliminated accumulation of the occupied receptors in the coated invaginations. Replacement of Tyr(960) in the NPEY domain with phenylalanine (CHOF960) had no significant effect on insulin internalization, receptor mobility, aggregation or accumulation in coated invaginations. In contrast, replacement of Tyr(960) with alanine (CHOA960) decreased insulin internalization, slowed migration, receptor aggregation and accumulation in coated imaginations. These studies document that kinase activity is required, but not sufficient, for receptor movement from the microvilli and aggregation of occupied receptors on the non-villous surface. An intact NPEY motif or surrounding amino acids, but not the phosphorylation of Tyr(960) plays a role in receptor mobility and aggregation and is essential for the accumulation of insulin receptors in coated invaginations. C1 JOSLIN DIABET CTR,BOSTON,MA 02215. RP Smith, RM (reprint author), UNIV PENN,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104, USA. FU NIDDK NIH HHS [DK43808, DK28143] NR 22 TC 6 Z9 8 U1 0 U2 1 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 SN 1079-9893 J9 J RECEPT SIGNAL TR R JI J. Recept. Signal Transduct. Res. PY 1996 VL 16 IS 5-6 BP 339 EP 355 DI 10.3109/10799899609039955 PG 17 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA VY273 UT WOS:A1996VY27300005 PM 8968965 ER PT J AU Badr, IA AlRajhi, A Wagoner, MD Dunham, T Teichmann, KD Cameron, JA AF Badr, IA AlRajhi, A Wagoner, MD Dunham, T Teichmann, KD Cameron, JA TI Phototherapeutic keratectomy for climatic droplet keratopathy SO JOURNAL OF REFRACTIVE SURGERY LA English DT Article ID EXCIMER-LASER; FOLLOW-UP AB BACKGROUND: Phototherapeutic keratectomy (PTK) is an effective treatment for many superficial corneal disorders. The efficacy of PTK for the treatment of climatic droplet keratopathy (CDK) has not been reported. METHODS: We report the results of excimer laser (Summit Technology, Inc, Waltham, Mass) PTK on 75 eyes (67 patients) with ''smooth'' climatic droplet keratopathy (55 eyes) and ''irregular'' climatic droplet keratopathy (20 eyes) in whom more than 6 months of follow up are available. RESULTS: PTK was successful in reducing corneal opacification in both smooth (98%) climatic droplet keratopathy and irregular (80%) climatic droplet keratopathy. Achievement of a clear or mildly hazy cornea following PTK was more likely to occur with smooth (80%) climatic droplet keratopathy than irregular (25%) climatic droplet keratopathy (P=0.01). Eyes with smooth climatic droplet keratopathy were more likely to obtain more than one line of improved uncorrected (56% vs. 25%) or spectacle-corrected visual acuity (61.8% vs. 21.2%) than those with irregular climatic droplet keratopathy (P=0.03 and 0.005, respectively). Delayed re-epithelialization (longer than 14 days) was more common in irregular CDK (21%) than in smooth CDK (9%), as was the incidence of secondary microbial keratitis (10.0% vs. 1.8%). CONCLUSIONS: PTK is effective in reducing superficial corneal opacification in CDK, although serious complications may occur, especially in advanced irregular CDK. C1 KING KHALID EYE SPECIALIST HOSP,ANTERIOR SEGMENT EXTERNAL DIS DIV,RIYADH 11462,SAUDI ARABIA. KING SAUD UNIV,SCH MED,DEPT OPHTHALMOL,RIYADH,SAUDI ARABIA. MASSACHUSETTS EYE & EAR INFIRM,CORNEA SERV,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT OPHTHALMOL,BOSTON,MA. NR 24 TC 10 Z9 11 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 J9 J REFRACT SURG JI J. Refractive Surg. PD JAN-FEB PY 1996 VL 12 IS 1 BP 114 EP 122 PG 9 WC Ophthalmology; Surgery SC Ophthalmology; Surgery GA TU355 UT WOS:A1996TU35500020 PM 8963799 ER PT J AU AlRajhi, AA Wagoner, MD Badr, IA AlSaif, A Mahmood, M AF AlRajhi, AA Wagoner, MD Badr, IA AlSaif, A Mahmood, M TI Bacterial keratitis following phototherapeutic keratectomy SO JOURNAL OF REFRACTIVE SURGERY LA English DT Article ID EXCIMER-LASER AB BACKGROUND: Phototherapeutic keratectomy (PTK) is effective in the treatment of many superficial corneal disorders. The incidence of bacterial keratitis following PTK has not been assessed in a large, prospective clinical trial. METHODS: We report three cases of bacterial keratitis that occurred in a prospective study of 258 consecutive PTK procedures at King Khaled Eye Specialist Hospital. RESULTS: Three (1.2%) of 258 eyes developed bacterial keratitis during a period of follow up ranging from 1 to 24 months. All three cases were in 183 eyes (1.6%) with a diagnosis of climatic droplet keratopathy, while no cases were observed in 75 eyes with other anterior corneal disorders. Gram-positive species (Streptococcus pneumonia in two, coagulase-negative Staphylococcus in one) were the predominant species isolated from all three cases. Two of the cases were polybacterial. The final visual outcomes ranged from 20/125 to 20/400. CONCLUSIONS: The risk. of bacterial keratitis following treatment of superficial corneal disorders with PTK is low but its occurrence may adversely affect the final visual outcome. C1 KING KHALID EYE SPECIALIST HOSP,EXTERNAL DIS DIV,RIYADH 11462,SAUDI ARABIA. KING KHALID EYE SPECIALIST HOSP,RES DEPT,RIYADH 11462,SAUDI ARABIA. KING SAUD UNIV,SCH MED,DEPT OPHTHALMOL,RIYADH,SAUDI ARABIA. HARVARD UNIV,SCH MED,DEPT OPHTHALMOL,BOSTON,MA. MASSACHUSETTS EYE & EAR INFIRM,CORNEA SERV,BOSTON,MA. RP AlRajhi, AA (reprint author), KING KHALID EYE SPECIALIST HOSP,MED LIB,ANTERIOR SEGMENT DIV,POB 7191,RIYADH 11462,SAUDI ARABIA. NR 25 TC 9 Z9 11 U1 0 U2 0 PU SLACK INC PI THOROFARE PA 6900 GROVE RD, THOROFARE, NJ 08086 J9 J REFRACT SURG JI J. Refractive Surg. PD JAN-FEB PY 1996 VL 12 IS 1 BP 123 EP 127 PG 5 WC Ophthalmology; Surgery SC Ophthalmology; Surgery GA TU355 UT WOS:A1996TU35500021 PM 8963801 ER PT J AU Arnason, JA Patel, AK Rahko, PS Sundstrom, WR AF Arnason, JA Patel, AK Rahko, PS Sundstrom, WR TI Transthoracic and transesophageal echocardiographic evaluation of the aortic root and subvalvular structures in ankylosing spondylitis SO JOURNAL OF RHEUMATOLOGY LA English DT Article DE ankylosing spondylitis; cardiac disease; aortic insufficiency; transesophageal echocardiography ID ABNORMALITIES; REGURGITATION AB Objective. During the preclinical phase of cardiac involvement in ankylosing spondylitis (AS), examination, electrocardiography, and transthoracic echocardiography (TTE) may lack the sensitivity to detect cardiac abnormalities. Since transesophageal echocardiography (TEE) allows a closer view of the aortic root and subvalvular structures we investigated whether preclinical abnormalities of the aortic root and subvalvular structures could be detected. Methods. Clinical and echocardiographic (TTE and TEE) evaluation of 29 male patients with AS and 13 age matched controls. Results. No patient with AS had a high degree heart block. Aortic root dimensions were comparable between the study groups, but the anterior aortic wall was thinner in patients with AS than controls, 0.25 and 0.41 cm, respectively (p = 0.016). The posterior aortic wall was thicker and subjectively more echogenic than the anterior wall in 17/29 patients with AS compared to 4/13 controls. Aortic valve insufficiency was detected with TEE in 10/29 patients with AS. In 8/9 patients with AS studied with TEE, the subaortic structures were thickened and/or of increased echogenicity. This abnormal echo extended into the membranous septum. Conclusion. Abnormal subvalvular echoes consistent with fibrosis of the aortic root and membranous interventricular septum were detected with TEE but not TTE, The use of TEE may allow earlier diagnosis of cardiac involvement in AS. C1 WILLIAM S MIDDLETON MEM VET ADM MED CTR,RHEUMATOL SECT,DEPT MED,MADISON,WI. WILLIAM S MIDDLETON MEM VET ADM MED CTR,CARDIOL SECT,DEPT MED,MADISON,WI. UNIV WISCONSIN HOSP & CLIN,MADISON,WI. NR 19 TC 13 Z9 15 U1 0 U2 0 PU J RHEUMATOL PUBL CO PI TORONTO PA 920 YONGE ST, SUITE 115, TORONTO ON M4W 3C7, CANADA SN 0315-162X J9 J RHEUMATOL JI J. Rheumatol. PD JAN PY 1996 VL 23 IS 1 BP 120 EP 123 PG 4 WC Rheumatology SC Rheumatology GA TN514 UT WOS:A1996TN51400021 PM 8838519 ER PT J AU DamronRodriguez, JA AF DamronRodriguez, JA TI International handbook on services for the elderly - Kosberg,JI SO JOURNAL OF SOCIAL SERVICE RESEARCH LA English DT Book Review RP DamronRodriguez, JA (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,GRECC,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU HAWORTH PRESS INC PI BINGHAMTON PA 10 ALICE ST, BINGHAMTON, NY 13904-1580 SN 0148-8376 J9 J SOC SERV RES JI J. Soc. Serv. Res. PY 1996 VL 21 IS 3 BP 73 EP 74 DI 10.1300/J079v21n03_05 PG 2 WC Social Work SC Social Work GA UY943 UT WOS:A1996UY94300005 ER PT J AU Choi, HS Seol, WG Moore, DD AF Choi, HS Seol, WG Moore, DD TI A component of the 26S proteasome binds an orphan member of the nuclear hormone receptor superfamily SO JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY LA English DT Article; Proceedings Paper CT 12th International Symposium of the Journal-of-Steroid-Biochemistry-and-Molecular-Biology CY MAY 21-24, 1995 CL BERLIN, GERMANY SP J Steroid Biochem & Molec Biol ID 26-S PROTEASE; FAMILY; ENCODES; DOMAIN AB The 26S proteasome complex plays a general role in turnover of both short and long lived proteins by specifically degrading ubiquitinated proteins. Recent evidence suggests that this large protease has more specific functions in a number of important cellular processes, ranging from activation of the transcription factor NFkB and antigen processing to transit through mitosis. We have identified a component of the 26S proteasome that interacts specifically with MB67, an orphan member of the nuclear hormone receptor superfamily. MIP224 (MB67 interacting protein) was isolated using the yeast two hybrid system and is apparently identical to the human 26S proteasome component TBP7. MIP224/TBP7 is one of several proteasomal proteins that share a strongly conserved ATPase domain (CAD) which is also present in a rapidly expanding superfamily of proteins with diverse functions. In yeast, MIP224 interacts specifically with MB67 and another closely related orphan receptor, but does not interact with several other receptor superfamily members tested. In mammalian cells, coexpression of MIP224 inhibits transactivation by MB67. MIP224 also interacts in yeast with other CAD proteins, including MSS1, which is proteasomal, and TRIP1, which is associated with transcriptional activation. This interaction of a proteasomal protein with a transcriptional protein suggests a previously unexpected link between the processes of protein degradation and transcriptional regulation. C1 MASSACHUSETTS GEN HOSP,DEPT MOLEC BIOL,BOSTON,MA 02114. FU NIDDK NIH HHS [DK46546] NR 33 TC 19 Z9 23 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0960-0760 J9 J STEROID BIOCHEM JI J. Steroid Biochem. Mol. Biol. PD JAN PY 1996 VL 56 IS 1-6 BP 23 EP 30 DI 10.1016/0960-0760(95)00220-0 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA UC701 UT WOS:A1996UC70100004 PM 8603043 ER PT J AU Krahn, D Piper, D King, M Olson, L Kurth, C Moberg, DP AF Krahn, D Piper, D King, M Olson, L Kurth, C Moberg, DP TI Dieting in sixth grade predicts alcohol use in ninth grade SO JOURNAL OF SUBSTANCE ABUSE LA English DT Article ID BULIMIA-NERVOSA; ANOREXIA-NERVOSA; SUBSTANCE-ABUSE; WOMEN; ADOLESCENTS; DISORDER; DEPRESSION; BEHAVIOR AB Recent studies of community-based populations have shown that the comorbidity seen in clinical studies of individuals with eating disorders and substance abuse extends in a graded manner to subclinical levels of each dysfunction as well as to adolescent populations. We hypothesized that frequency of dieting in the sixth grade would predict later alcohol use in middle school students. Data from 1,905 participants in a middle school health promotion project were analyzed We found a positive, graded relationship between the frequency of dieting in the sixth grade and the frequency of alcohol intake in the ninth grade. We also found that frequency of dieting in sixth grade was a more powerful predictor of future drinking than such parameters as others' approval of alcohol use, perceptions of peer use of alcohol, and personal feelings of shyness and self-satisfaction. Implications of these findings are discussed. C1 UNIV WISCONSIN,SCH MED,MADISON,WI 53706. UNIV MICHIGAN,ANN ARBOR,MI 48109. RP Krahn, D (reprint author), UNIV WISCONSIN,WILLIAM S MIDDLETON MEM VET HOSP,SCH MED,2500 OVERLOOK TERRACE,MADISON,WI 53705, USA. NR 27 TC 22 Z9 22 U1 2 U2 5 PU ABLEX PUBL CORP PI NORWOOD PA 355 CHESTNUT ST, NORWOOD, NJ 07648 SN 0899-3289 J9 J SUBST ABUSE JI J. SUBST. ABUSE PY 1996 VL 8 IS 3 BP 293 EP 301 DI 10.1016/S0899-3289(96)90161-3 PG 9 WC Substance Abuse SC Substance Abuse GA VU219 UT WOS:A1996VU21900002 PM 8934435 ER PT J AU Puria, S Guinan, JJ Liberman, MC AF Puria, S Guinan, JJ Liberman, MC TI Olivocochlear reflex assays: Effects of contralateral sound on compound action potentials versus ear-canal distortion products SO JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA LA English DT Article ID COCHLEAR MICROMECHANICAL PROPERTIES; SUPERIOR OLIVARY COMPLEX; MEDIAL EFFERENT SYSTEM; AUDITORY-NERVE FIBERS; GUINEA-PIG; ACOUSTIC DISTORTION; OTOACOUSTIC EMISSIONS; RESPONSE PROPERTIES; STIMULATION; NEURONS AB The strength of the olivocochlear reflex has been assayed by comparing ipsilateral cochlear responses with and without contralateral sound. In-humans, ipsilateral cochlear responses have usually been inferred by measuring otoacoustic emissions (OAEs), whereas, in animal work, they have been assessed by measuring compound action potentials (CAPs). Thus reports that the reflex strength is smaller in humans than in animals cannot be interpreted until the differences between the two tests are better understood. The present study directly compares reflex assays using distortion-product (DP) OAE and CAP measures in the same animals. For ipsilateral frequencies of 2-8 kHz and levels from 25 to 80 dB SPL, efferent reflex strength was computed from the CAP or DPOAE amplitude-versus-level curve's measured with and without contralateral noise. The ''effective attenuation'' produced by efferent activation was, with few exceptions, greater when measured with the CAP than with the DPOAE assay. Differences between the two measures increased as frequency increased, with differences as large as 10 dB observed. These results, coupled with previous measurements on humans and animals, suggest that the efferent reflex is at least as strong in humans as has been shown in animal experiments. (C) 1996 Acoustical Society of America. C1 MIT,ELECTR RES LAB,CAMBRIDGE,MA 02139. MIT,DEPT ELECT ENGN & COMP SCI,CAMBRIDGE,MA 02139. HARVARD UNIV,MIT,DIV HLTH SCI & TECHNOL,CAMBRIDGE,MA 02139. HARVARD UNIV,SCH MED,DEPT OTOL & LARYNGOL,BOSTON,MA 02115. RP Puria, S (reprint author), MASSACHUSETTS EYE & EAR INFIRM,EATON PEABODY LAB AUDITORY PHYSIOL,243 CHARLES ST,BOSTON,MA 02114, USA. FU NIDCD NIH HHS [F32 DC 00073, R0 DC 00235, R01 DC 00188] NR 48 TC 67 Z9 71 U1 0 U2 1 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0001-4966 J9 J ACOUST SOC AM JI J. Acoust. Soc. Am. PD JAN PY 1996 VL 99 IS 1 BP 500 EP 507 DI 10.1121/1.414508 PG 8 WC Acoustics; Audiology & Speech-Language Pathology SC Acoustics; Audiology & Speech-Language Pathology GA TQ787 UT WOS:A1996TQ78700051 PM 8568037 ER PT J AU Schwammenthal, E Chen, CG Giesler, M Sagie, A Guerrero, JL dePrada, JAV Hombach, V Weyman, AE Levine, RA AF Schwammenthal, E Chen, CG Giesler, M Sagie, A Guerrero, JL dePrada, JAV Hombach, V Weyman, AE Levine, RA TI New method for accurate calculation of regurgitant flow rate based on analysis of Doppler color flow maps of the proximal flow field - Validation in a canine model of mitral regurgitation with initial application in patients SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID SURFACE-AREA METHOD; CONVERGENCE REGION; VELOCITY PROFILE; VALVULAR REGURGITATION; NONINVASIVE ESTIMATION; VALVE REPAIR; ORIFICE SIZE; INVITRO; QUANTIFICATION; VOLUME AB Objectives. The purpose of this study was to develop a rational and objective method for selecting a region in the proximal Row field where the hemispheric formula for calculating regurgitant Bow rates by the flow convergence technique is most accurate. Background. A major obstacle to clinical implementation of the proximal flow convergence method is that it assumes hemispheric isovelocity contours throughout the Doppler color Bow map, whereas contour shape depends critically on location in the flow field. Methods. Twenty mitral regurgitant flow rate stages were produced in six dogs by implanting grommet orifices into the anterior mitral leaflet and varying driving pressures so that actual peak Bow rate could be determined from the known effective regurgitant orifice times the orifice velocity. Because plotting flow rate calculated by using a hemispheric formula versus alias velocities produces underestimation near the orifice and overestimation far from it, this plot was fitted to a polynomial function to allow identification of an inflection point within a relatively flat intermediate zone, where factors causing overestimation and underestimation are expected to be unimportant or balanced. The accuracy of Bow rate calculation by the inflection point was compared with unselective and selective averaging techniques, Clinical relevance, initial feasibility and correlation with an independent measure were tested in 13 consecutive patients with mitral regurgitation who underwent cardiac catheterization. Results. 1) The accuracy of single point calculations,was improved by selecting points in the flat portion of the curve (y = 1.15x - 3.34, r = 0.87, SEE = 22.1 ml/s vs. y = 1.34x - 1.99, r = 0.71, SEE = 45.6 ml/s, p < 0.01). 2) Selective averaging of points in the flat portion of the curve further improved accuracy and decreased scatter compared with unselective averaging (y = 1.08x + 4.8, r = 0.96, SEE = 11.6 ml/s vs, y = 1.30x + 0.6, r = 0.90, SEE = 20.9 mis, p < 0.01). 3) The proposed algorithm for mathematically identifying the inflection point provided the best results (y = 0.96x + 4.5, r = 0.96, SEE = 9.9 ml/s), with a mean error of 1.6 +/- 9.7 ml/s vs. 11.4 +/- 11.7 ml/s for selective averaging (p < 0.01), In patients, the proposed algorithm identified an inflection point at which calculated regurgitant volume agreed best with invasive measurements (y = 1.1x - 0.61, r = 0.93, SEE = 17 ml). Conclusions. The accuracy of the proximal flow convergence method can be significantly improved by analyzing the flow field mathematically to identify the optimal isovelocity zone before using the hemispheric formula to calculate regurgitant how rates. Because the proposed algorithm is objective, operator independent and, thus, suitable for automatization, it could provide the clinician with a powerful quantitative tool to assess valvular regurgitation. C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA. UNIV ULM,DIV CARDIOL,DEPT INTERNAL MED,W-7900 ULM,GERMANY. RP Schwammenthal, E (reprint author), MASSACHUSETTS GEN HOSP,CARDIAC ULTRASOUND LAB,VBK 508,BOSTON,MA 02114, USA. RI Guerrero, Jorge/I-3666-2015 OI Guerrero, Jorge/0000-0003-4315-7318 FU NHLBI NIH HHS [HL53702] NR 63 TC 45 Z9 46 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD JAN PY 1996 VL 27 IS 1 BP 161 EP 172 DI 10.1016/0735-1097(95)00428-9 PG 12 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA TN261 UT WOS:A1996TN26100023 PM 8522691 ER PT J AU Yoshikawa, TT Norman, DC AF Yoshikawa, TT Norman, DC TI Approach to fever and infection in the nursing home SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article ID TERM-CARE FACILITY; URINARY-TRACT INFECTION; LONG-TERM; PNEUMOCOCCAL VACCINE; ACQUIRED PNEUMONIA; ASYMPTOMATIC BACTERIURIA; NOSOCOMIAL INFECTIONS; CLINICAL-FEATURES; ELDERLY PATIENTS; BACTEREMIA AB OBJECTIVE: To summarize current information on the scope, epidemiology, clinical manifestations, diagnostic approach, and general management of infectious diseases in nursing home residents, as well as the specific treatment of common infections occurring in the nursing home setting. DESIGN: Survey and literature review of the diagnostic and therapeutic problems of nursing home residents with infections. CONCLUSIONS: Older persons residing in nursing homes as well as other types of long-term care facilities are at increased risk for infections. Moreover, infection is the most frequent reason for patients to be transferred from nursing homes to an acute-care facility. The most common infections that are acquired in nursing homes are urinary tract infection (cystitis pyelonephritis), respiratory infections (pneumonia, bronchitis), and skin/soft tissue infections (infected pressure ulcers, cellulitis). Most serious infections in this setting are caused by bacteria; however, influenza and other respiratory viruses as well as herpes tester may cause significant morbidity in older nursing home residents. Mycobacterium tuberculosis infects nursing home residents at a higher rate than it infects older community dwellers. Infections in older nursing home residents may manifest clinically, with atypical symptoms and signs, including the absence of fever. Rapid diagnostic evaluation and early therapeutic intervention are essential for minimizing the high mortality and morbidity associated with infections in this older population; most nursing home residents with serious infections should be considered for hospitalization. C1 W LOS ANGELES VET AFFAIRS MED CTR,GERIATR RES EDUC & CLIN CTR W11-G,LOS ANGELES,CA. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA 90024. RP Yoshikawa, TT (reprint author), CHARLES R DREW UNIV MED & SCI,DEPT INTERNAL MED MP-11,KING DREW MED CTR,12021 S WILMINGTON AVE,LOS ANGELES,CA 90059, USA. NR 87 TC 40 Z9 41 U1 1 U2 3 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD JAN PY 1996 VL 44 IS 1 BP 74 EP 82 PG 9 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA TP328 UT WOS:A1996TP32800013 PM 8537596 ER PT J AU Mathisen, DJ Wain, JC Wright, C Choi, N Carey, R Hilgenberg, A Grossbard, M Lynch, T Grillo, H AF Mathisen, DJ Wain, JC Wright, C Choi, N Carey, R Hilgenberg, A Grossbard, M Lynch, T Grillo, H TI Assessment of preoperative accelerated radiotherapy and chemotherapy in stage IIIA (N2) non-small-cell lung cancer SO JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY LA English DT Article; Proceedings Paper CT 75th Annual Meeting of the American-Association-for-Thoracic-Surgery CY APR 23-26, 1995 CL BOSTON, MA SP Amer Assoc Thorac Surg ID NODES AB Forty patients with N2 non-small-cell lung cancer (stage IIIA), as determined by mediastinoscopy, were entered into a preoperative neoadjuvant study of chemotherapy (platinum, 5-fluorouracil, vinblastine) and accelerated radiotherapy (150 cGy twice per day for 7 days) for two cycles. Surgical resection was then performed and followed up with an additional cycle of chemotherapy and radiotherapy. All patients completed preoperative therapy. A major clinical response was seen in 87% of patients. Thirty-five patients underwent resection (one preoperative death, one refused operation, one had deterioration of pulmonary function, and two had pleural metastases). Operative mortality rate was 5.7% (2/35). Sixty percent of patients had no complications. Major complications included pulmonary emboli (three), pneumonia (two), and myocardial infarction (one). Down-staging was seen in 46% of patients, with two patients (5.7%) having no evidence of tumor in the specimen, five patients having sterilization of all lymph nodes, and nine patients having sterilization of mediastinal nodes but positive N1 nodes. Median survival of 40 patients was 28 months, with a projected 5-year survival of 43%. Patients with downstaged disease had statistically significant improved survival compared with patients whose disease was not downstaged. RP MASSACHUSETTS GEN HOSP, SECT GEN THORAC SURG, WARREN 1109, BOSTON, MA 02114 USA. NR 13 TC 44 Z9 49 U1 0 U2 1 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-5223 EI 1097-685X J9 J THORAC CARDIOV SUR JI J. Thorac. Cardiovasc. Surg. PD JAN PY 1996 VL 111 IS 1 BP 123 EP 131 DI 10.1016/S0022-5223(96)70408-7 PG 9 WC Cardiac & Cardiovascular Systems; Respiratory System; Surgery SC Cardiovascular System & Cardiology; Respiratory System; Surgery GA TQ336 UT WOS:A1996TQ33600019 PM 8551756 ER PT J AU Kaufmann, MA Pargger, H Castelli, I Steiner, LA Drop, LJ AF Kaufmann, MA Pargger, H Castelli, I Steiner, LA Drop, LJ TI Renal vascular responses to high and low ionized calcium: Influence of norepinephrine in the isolated perfused rat kidney SO JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE LA English DT Article ID SEPTIC SHOCK; FAILURE; ACETYLCHOLINE; HYPOCALCEMIA; HEMODYNAMICS; ANTAGONISTS; INHIBITION; DILTIAZEM; PRESSURE; AFFERENT AB Objective and Design: The aim of this study was to examine the influence of norepinephrine (NE) on renal vascular responses to high (1.88 mmol/L and low (0.56 mmol/L) perfusate-ionized calcium ([Ca2+]) in the isolated perfused kidney of the rat. High and low [Ca2+] encompassed the clinical concentration range in this multiexperinent, randomized trial. Materials and Methods: Rats (n = 25), ranging in age from 3 to 4 months, were anesthetized and the ureter and renal artery were cannulated. The right kidney was perfused with oxygenated, warmed albumin (67 g/L) containing Krebs-Henseleit buffer and placed in a thermostated chamber without interruption of now. In protocol A (n = 7), steady-state high [Ca2+] (1.88 mmol/L) and low [Ca2+] (0.56 mmol/L) were instituted in randomized order in each experiment under basal conditions. In protocol B (n = 9), the same interventions were instituted during constant rate NE infusion. Changes in renal flow were measured at constant perfusion pressure (110 mm Hg), and renal vascular resistance (RVR) was calculated, Renal function was assessed by clearance of [C-14]inulin and by fractional excretion of sodium. With NE-induced preconstriction, the increase in RVR observed during high [Ca2+] was +17.8 +/- 1.8% of control, and the decrease in RVR observed during low [Ca2+] was -35.9 +/- 8.2% of control. Both values were greater by a factor of 2 than corresponding results obtained under basal conditions (7 +/- 2.1% vs. -13.5 +/- 4.1% of control, respectively, p < 0.05). Whereas the decrease in glomerular filtration rate with high [Ca2+] was not significantly influenced by NE pretreatment (-9 +/- 1.8% of control with high [Ca2+] in combination with NE vs. 4.1 +/- 0.7% of control under basal conditions), the increase in glomerular filtration rate with low [Ca2+] was significantly greater in the presence of NE (12 +/- 0.7 vs. 102 +/- 8.5% of control, p < 0.01). Conclusions: Whereas under basal conditions renal vascular effects of high and low [Ca2+] (varied within the clinical concentration range) are small, the changes recorded with the same interventions after NE pretreatment are increased by a factor of >2. Hypercalcemia-induced renovascular constriction and decreased function are unfavorable, especially in patients who are at risk for renal dysfunction from other causes. C1 MASSACHUSETTS GEN HOSP,DEPT ANESTHESIA,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,HENRY K BEECHER LAB,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT ANAESTHESIA,BOSTON,MA 02115. RI Steiner, Luzius/C-9836-2011 NR 36 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 1079-6061 J9 J TRAUMA JI J. Trauma-Injury Infect. Crit. Care PD JAN PY 1996 VL 40 IS 1 BP 110 EP 115 DI 10.1097/00005373-199601000-00020 PG 6 WC Critical Care Medicine; Surgery SC General & Internal Medicine; Surgery GA TU074 UT WOS:A1996TU07400022 PM 8576971 ER PT J AU McDougal, WS AF McDougal, WS TI No evidence of osteopenia 5 to 8 years after ileal orthotopic bladder substitution - Comment SO JOURNAL OF UROLOGY LA English DT Editorial Material RP McDougal, WS (reprint author), MASSACHUSETTS GEN HOSP,DEPT UROL,BOSTON,MA 02114, USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0022-5347 J9 J UROLOGY JI J. Urol. PD JAN PY 1996 VL 155 IS 1 BP 75 EP 75 PG 1 WC Urology & Nephrology SC Urology & Nephrology GA TJ434 UT WOS:A1996TJ43400022 ER PT J AU Marx, MV Williams, DM Perkins, AJ Reynolds, PI Nelson, VS Andrews, JC Bushey, LN AF Marx, MV Williams, DM Perkins, AJ Reynolds, PI Nelson, VS Andrews, JC Bushey, LN TI Percutaneous feeding tube placement in pediatric patients: Immediate and 30-day results SO JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY LA English DT Article DE children, gastrointestinal tract; feeding tube; gastrostomy; interventional procedures, in infants and children ID ANTERIOR GASTRIC WALL; NYLON T-FASTENER; ENDOSCOPIC GASTROSTOMY; GASTROJEJUNOSTOMY; GASTROENTEROSTOMY; EXPERIENCE; CHILDREN; FIXATION AB PURPOSE: To evaluate fluoroscopically guided percutaneous feeding tube placement in pediatric patients. MATERIALS AND METHODS: Sixty-one procedures were performed, Periprocedural care protocol was changed after patient nine. Forty-eight-hour and 30-day outcomes were assessed. RESULTS: Almost 97% of procedures were successful, The 48-hour major and minor complication rates were 1.9% and 9.6%, respectively, after the initial nine procedures. Risk factors for early complications were the use of the initial care protocol (P < .01) and patient weight below the 50th percentile (P < .05). Major and minor 30-day complication rates were 8.3% and 12.0%, respectively. Risk factors for delayed complications were placement of a gastrojejunostomy tube rather than a gastrostomy tube (P < .05) and immunosuppression (P < .05). CONCLUSION: Percutaneous feeding tubes can be placed in children with a high rate of technical success. Optimal results require attention to periprocedural care. Morbidity is common during the first month of tube use. C1 UNIV MICHIGAN HOSP,DEPT ANESTHESIOL,ANN ARBOR,MI 48109. UNIV MICHIGAN HOSP,DEPT PHYS MED & REHABIL,ANN ARBOR,MI 48109. US DEPT VET AFFAIRS,HLTH SERV RES & DEV FIELD PROGRAM,ANN ARBOR,MI. RP Marx, MV (reprint author), UNIV MICHIGAN HOSP,DEPT RADIOL,1500 E MED CTR DR,BOX 0030,ANN ARBOR,MI 48109, USA. NR 32 TC 9 Z9 9 U1 0 U2 0 PU LIPPINCOTT-RAVEN PUBL PI PHILADELPHIA PA 227 EAST WASHINGTON SQ, PHILADELPHIA, PA 19106 SN 1051-0443 J9 J VASC INTERV RADIOL JI J. Vasc. Interv. Radiol. PD JAN-FEB PY 1996 VL 7 IS 1 BP 107 EP 115 DI 10.1016/S1051-0443(96)70745-5 PG 9 WC Radiology, Nuclear Medicine & Medical Imaging; Peripheral Vascular Disease SC Radiology, Nuclear Medicine & Medical Imaging; Cardiovascular System & Cardiology GA UQ437 UT WOS:A1996UQ43700020 PM 8773984 ER PT J AU Paulus, W Baur, I Boyce, FM Breakefield, XO Reeves, SA AF Paulus, W Baur, I Boyce, FM Breakefield, XO Reeves, SA TI Self-contained, tetracycline-regulated retroviral vector system for gene delivery to mammalian cells SO JOURNAL OF VIROLOGY LA English DT Article ID ADENOSINE-DEAMINASE; EXPRESSION; PROMOTER AB Retroviral vectors that contain the tetracycline-inducible (Tet) system were developed, The two components of the Tet system were organized within the vectors in a manner that stringently maintains tetracycline-dependent regulation, Regulated expression of an indicator gene inserted into the retroviral vectors was examined in several different cell types, In infected NIH 3T3 cells, levels of induction in the absence of tetracycline were observed to be as much as 336-fold higher than levels in the presence of tetracycline, which were extremely low. Tetracycline-dependent regulation was observed in all other transduced cell types and ranged from 24- to 127-fold. The generation of retroviral vectors containing regulatory elements that allow for the regulated expression of heterologous genes and that have the ability to infect virtually all dividing target cells should greatly facilitate the biochemical and genetic examination of a broad range of genes. Moreover, these inducible retroviral vectors should prove useful in gene therapy applications. C1 MASSACHUSETTS GEN HOSP,CTR NEUROSCI,DEPT NEUROSURG,MOLEC NEUROONCOL & MOLEC NEUROGENET UNIT,BOSTON,MA 02129. MASSACHUSETTS GEN HOSP,CTR NEUROSCI,DEPT NEUROL,BOSTON,MA 02129. HARVARD UNIV,SCH MED,BOSTON,MA 02129. NR 22 TC 173 Z9 186 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1996 VL 70 IS 1 BP 62 EP 67 PG 6 WC Virology SC Virology GA TJ650 UT WOS:A1996TJ65000009 PM 8523582 ER PT J AU Kondo, E Gottlinger, HG AF Kondo, E Gottlinger, HG TI A conserved LXXLF sequence is the major determinant in p6(gag) required for the incorporation of human immunodeficiency virus type 1 Vpr SO JOURNAL OF VIROLOGY LA English DT Article ID ALANINE-SCANNING MUTAGENESIS; PROTEIN SECONDARY STRUCTURE; PRODUCTIVE INFECTION; MUTATIONAL ANALYSIS; HUMAN MACROPHAGES; GENE; GAG; EXPRESSION; PREDICTION; RELEASE AB The vpr gene product of human immunodeficiency virus type 1 (HIV-1) is a virion-associated regulatory protein. A transferable virion association motif for Vpr is located in the p6 domain of the HIV-1. Gag polyprotein. To map the sequences in p6 that are involved in Vpr incorporation, we analyzed the ability of mutant forms of p6 to direct the incorporation of Vpr into chimeric viral particles. Our results show that the determinants which govern Vpr incorporation are largely confined to a C-terminal region of the p6 domain. Within this region, three hydrophobic residues in a highly conserved sequence motif (L-X-S-L-F-G) are absolutely required. Remarkably, the transfer of the conserved motif and of a single flanking residue to a heterologous Gag polyprotein was sufficient to transfer the ability to incorporate Vpr at moderate levels. The transfer of residues 32 to 46 of p6 led to Vpr incorporation levels that were comparable to those obtained with full-length HIV-1 Gag protein, indicating that this region contains essentially all the information required for efficient Vpr incorporation. C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV HUMAN RETROVIROL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. FU NIAID NIH HHS [AI34267, AI28691, AI29873] NR 40 TC 83 Z9 83 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0022-538X J9 J VIROL JI J. Virol. PD JAN PY 1996 VL 70 IS 1 BP 159 EP 164 PG 6 WC Virology SC Virology GA TJ650 UT WOS:A1996TJ65000021 PM 8523520 ER PT J AU Ershler, WB AF Ershler, WB TI Editorial policy statement SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES LA English DT Editorial Material RP Ershler, WB (reprint author), UNIV WISCONSIN,GRECC,WILLIAM S MIDDLETON MEM VET ADM MED CTR,DEPT MED,SECT GERIATR,MADISON,WI 53705, USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GERONTOLOGICAL SOCIETY AMER PI WASHINGTON PA 1275 K STREET NW SUITE 350, WASHINGTON, DC 20005-4006 SN 1079-5006 J9 J GERONTOL A-BIOL JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci. PD JAN PY 1996 VL 51 IS 1 BP M1 EP M1 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA TP043 UT WOS:A1996TP04300016 ER PT J AU Schreiber, M Schlanger, LE Chen, CB LessanPezeshki, M Halperin, ML Patnaik, A Ling, BN Kleyman, TR AF Schreiber, M Schlanger, LE Chen, CB LessanPezeshki, M Halperin, ML Patnaik, A Ling, BN Kleyman, TR TI Antikaliuretic action of trimethoprim is minimized by raising urine pH SO KIDNEY INTERNATIONAL LA English DT Article ID TRANSTUBULAR POTASSIUM CONCENTRATION; HYPERKALEMIA; CHANNELS AB This study was designed to test the hypothesis that the antikaliuresis caused by trimethoprim could be diminished by alkalinizing the luminal fluid in the CCD, thereby converting trimethoprim from its cationic, active form to an electroneutral, inactive form. Timethoprim-induced inhibition of transepithelial Na+ transport was examined in A6 distal nephron cells by analysis of short circuit current. The voltage-dependence of the trimethoprim-induced block of Na+ channels was examined with patch clamp recordings of A6 cells. The antikaliuretic effect of trimethoprim was examined in vivo in rats pretreated with desoxycorticosterone and with NH4Cl to lower urine pH, and in rats also receiving acetazoiamide to raise urine pH. We found that the concentration of trimethoprim required to inhibit the amiloride sensitive component of short circuit current by 50% (IC50) was 340 mu M (at pH 8.2) and 50 mu M (at pH 6.3). The IC(50)s of protonated trimethoprim were similar (34 mu M at PH 8.2 and 45 mu M at pH 6.3). The mean time open for the high selectivity, Na+ channel was reduced from 1679 +/- 387 msec to 502 +/- 98 msec with addition of 10(-5) M trimethoprim to patch pipette solution at the resting membrane potential (-V-pipette = 0 mV). Further decreases in mean time open were observed as -V-pipette was reduced (that is, apical membrane hyperpolarization) to -40 mV (mean time open = 217 +/- 85 msec) and to -80 mV (mean time open = 69 +/- 13 msec). In vivo, trimethoprim caused a > 50% reduction in potassium (K+) excretion due primarily to a fall in the [K+] in the lumen of the terminal CCD. This effect of trimethoprim was markedly attenuated in an alkaline urine induced by acetazolamide. We conclude that it is the charged, protonated species of trimethoprim which blocks epithelial Na+ channels. Increasing urinary pH decreases the concentration of the charged species of trimethoprim and minimizes its antikaliuretic effect. C1 UNIV TORONTO,ST MICHAELS HOSP,DEPT MED,TORONTO,ON M5B 1A6,CANADA. EMORY UNIV,DEPT MED,DIV RENAL,ATLANTA,GA 30322. VET AFFAIRS MED CTR,ATLANTA,GA. UNIV PENN,DEPT MED,PHILADELPHIA,PA 19104. UNIV PENN,DEPT PHYSIOL,PHILADELPHIA,PA 19104. VET AFFAIRS MED CTR,PHILADELPHIA,PA. FU NIDDK NIH HHS [T32-DK07656, K08-DK02111] NR 29 TC 19 Z9 20 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD JAN PY 1996 VL 49 IS 1 BP 82 EP 87 DI 10.1038/ki.1996.11 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA TM411 UT WOS:A1996TM41100011 PM 8770952 ER PT J AU Pahl, M Jara, A Bover, J Rodriguez, M Felsenfeld, AJ AF Pahl, M Jara, A Bover, J Rodriguez, M Felsenfeld, AJ TI The set point of calcium and the reduction of parathyroid hormone in hemodialysis patients SO KIDNEY INTERNATIONAL LA English DT Article ID SECONDARY HYPERPARATHYROIDISM; RENAL-FAILURE; INTRAVENOUS CALCITRIOL; SIGMOIDAL RELATIONSHIP; GLAND FUNCTION; BONE-DISEASE; SUPPRESSION; CALCITONIN; SECRETION; DIALYSIS AB Since in some studies in hemodialysis patients calcitriol treatment has resulted in a reduction of both parathyroid hormone (PTH) levels and the set point of calcium, it has been suggested that the set point of calcium reflects a reduction in the magnitude of hyperparathyroidism. However, others have maintained that the set point of calcium is primarily an indicator of the serum calcium at which PTH is secreted and may be dissociated from the magnitude of hyperparathyroidism. The present study was designed to evaluate how a reduction in PTH levels associated with an increase in the predialysis (basal) serum calcium would affect the set point of calcium. Two different treatments were used to produce a reduction in PTH that was associated with an increase in predialysis serum calcium. In the first group, hemodialysis patients received 2 mu g of intravenous calcitriol and were dialyzed with a 3.5 mEq/liter calcium dialysate for six weeks; in the second group, hemodialysis patients were dialyzed with a 4 mEq/liter calcium dialysate and had oral calcium supplementation increased for six weeks. In both groups, low and high calcium studies were performed to determine the PTH-calcium relationship before treatment, at the end of six weeks of treatment, and six weeks after the discontinuation of treatment. In the calcitriol group, the predialysis calcium increased from 9.62 +/- 0.34 to 10.56 +/- 0.31 mg/dl, P < 0.05 and the set point of calcium increased from 9.34 +/- 0.23 to 9.79 +/- 0.25 mg/dl, P < 0.05 at the same time as maximally stimulated PTH decreased from 2637 +/- 687 to 1555 +/- 617 pg/ml, P < 0.05. In the high calcium dialysate group, the predialysis serum calcium increased from 9.19 +/- 0.31 to 9.84 +/- 0.28 mg/dl, P < 0.05, and set point of calcium increased from 9.01 +/- 0.28 to 9.39 +/- 0.22 mg/dl, P < 0.05 at the same time as maximally stimulated PTH decreased from 1642 +/- 450 to 1349 +/- 513 pg/ml, P < 0.05. Discontinuation of treatment for six weeks resulted in a return to pretreatment values. In conclusion, our results would suggest that (1) the set point of calcium may not be a reliable indicator of the magnitude of hyperparathyroidism during calcitriol treatment, and (2) PTH secretion may adapt to the ambient serum calcium concentration. C1 UNIV CALIF LOS ANGELES,W LOS ANGELES VET AFFAIRS MED CTR,DEPT MED,NEPHROL SECT W111L,LOS ANGELES,CA 90073. UNIV CALIF IRVINE,DEPT MED,IRVINE,CA 92717. HOSP REINA SOFIA,UNIT INVEST,CORDOBA,SPAIN. RI Rodriguez, teresa/H-5452-2011 NR 31 TC 37 Z9 37 U1 0 U2 1 PU BLACKWELL SCIENCE INC PI CAMBRIDGE PA 238 MAIN ST, CAMBRIDGE, MA 02142 SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD JAN PY 1996 VL 49 IS 1 BP 226 EP 231 DI 10.1038/ki.1996.31 PG 6 WC Urology & Nephrology SC Urology & Nephrology GA TM411 UT WOS:A1996TM41100031 PM 8770972 ER PT J AU Keel, SB Koerner, FC Efird, JT Bhan, AK Rosenberg, AE AF Keel, SB Koerner, FC Efird, JT Bhan, AK Rosenberg, AE TI Estrogen and progesterone receptor status and disease specific survival in skull base chordomas SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 24 EP 24 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700055 ER PT J AU Nielsen, GP Rosenberg, AE Liebsch, NJ AF Nielsen, GP Rosenberg, AE Liebsch, NJ TI Chordoma of the base of skull ln children and adolecents. A clinicopathologic study of 35 cases. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 44 EP 44 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700075 ER PT J AU Rosenberg, AE Nielsen, GP Efird, JT Liebsch, NJ AF Rosenberg, AE Nielsen, GP Efird, JT Liebsch, NJ TI Base of skull chondrosarcomas. A clinicopathologic study of 130 cases. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 52 EP 52 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700083 ER PT J AU Rosenberg, AE Amstalden, EMI Nielsen, GP Efird, JT Ferguson, WS Harmon, DC Spiro, IJ ONeilSmith, K Mankin, HJ AF Rosenberg, AE Amstalden, EMI Nielsen, GP Efird, JT Ferguson, WS Harmon, DC Spiro, IJ ONeilSmith, K Mankin, HJ TI Ewing's sarcoma PNET: Preoperative chemotherapy induced necrosis and outcome SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 53 EP 53 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700084 ER PT J AU Pennella, R Crowson, AN Magro, CM AF Pennella, R Crowson, AN Magro, CM TI The dermatopathology of mixed connective tissue disease SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 BETH ISRAEL HOSP,CAMBRIDGE,MA. PATHOL SERV INC,CAMBRIDGE,MA. MISERICORDIA GEN HOSP,CENT MED LABS,WINNIPEG,MB,CANADA. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 241 EP 241 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700272 ER PT J AU Lipman, JK Mishra, R Hill, DE Jessup, JM Kolodner, R Loda, M AF Lipman, JK Mishra, R Hill, DE Jessup, JM Kolodner, R Loda, M TI Mismatch repair gene hMLH1 expression in colorectal carcinoma SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 DEACONESS HOSP,DEPT PATHOL,BOSTON,MA. DEACONESS HOSP,DEPT SURG,BOSTON,MA. HARVARD UNIV,SCH MED,DANA FARBER CANC CTR,BOSTON,MA. ONCOGENE SCI INC,CAMBRIDGE,MA. RI Hill, David/B-6617-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 344 EP 344 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700375 ER PT J AU Torres, C Rustgi, A Proulx, G Wang, H Shahsafaei, A Odze, R AF Torres, C Rustgi, A Proulx, G Wang, H Shahsafaei, A Odze, R TI Cyclin D1 expression in esophageal squamous cell carcinoma: Correlation with survival SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT PATHOL,BOSTON,MA 02115. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT PATHOL,BOSTON,MA. HARVARD UNIV,BETH ISRAEL HOSP,SCH MED,DEPT PATHOL,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 377 EP 377 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700408 ER PT J AU Grignon, D Won, M Hammond, E Dolan, P Lawton, C Mesic, J Fu, K Porter, A Abrams, R Shipley, W AF Grignon, D Won, M Hammond, E Dolan, P Lawton, C Mesic, J Fu, K Porter, A Abrams, R Shipley, W TI DNA content as a prognostic indicator in prostate cancer (PCA): A study based on RTOG protocol 8610. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 KARMANCS CANC INST,DETROIT,MI. WAYNE STATE UNIV,DETROIT,MI 48202. RADIAT THERAPY ONCOL GRP,PHILADELPHIA,PA. LATTER DAY ST HOSP,SALT LAKE CITY,UT 84143. MED COLL WISCONSIN,MILWAUKEE,WI 53226. SACRAMENTO RADIOL GRP,SACRAMENTO,CA. UNIV CALIF SAN FRANCISCO,SAN FRANCISCO,CA 94143. JOHNS HOPKINS UNIV,BALTIMORE,MD 21218. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 419 EP 419 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700450 ER PT J AU Jones, EC Young, RH AF Jones, EC Young, RH TI Myxoid and sclerosing sarcomatoid transitional cell carcinoma (STCC) of the urinary bladder: A study of 23 cases. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV BRITISH COLUMBIA,VANCOUVER,BC V5Z 1M9,CANADA. VANCOUVER HOSP & HLTH SCI CTR,VANCOUVER,BC,CANADA. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 427 EP 427 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700458 ER PT J AU Jones, MA Young, RH Scully, RE AF Jones, MA Young, RH Scully, RE TI Benign fibromatous tumors of the testis SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MAINE MED CTR,PORTLAND,OR. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 428 EP 428 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700459 ER PT J AU Koelliker, DD Young, RH Scully, RE AF Koelliker, DD Young, RH Scully, RE TI Testicular sertoli cell tumours (not otherwise specified): A clinicopathologic analysis of 52 cases. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 433 EP 433 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700464 ER PT J AU Oliva, E Young, RH AF Oliva, E Young, RH TI Urethral clear cell adenocarcinoma: A study of 19 cases. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 455 EP 455 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700486 ER PT J AU True, LD Grignon, DJ Hammond, EH Earle, JD Parliament, M Byhardt, R Tester, W Heaney, NM Pajak, TJ AF True, LD Grignon, DJ Hammond, EH Earle, JD Parliament, M Byhardt, R Tester, W Heaney, NM Pajak, TJ TI Staging of bladder cancer in RTOG trials: Role of central review in 88-02 and 89-03 SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 UNIV WASHINGTON,SEATTLE,WA 98195. WAYNE STATE UNIV,DETROIT,MI 48202. LATTER DAY ST HOSP,SALT LAKE CITY,UT 84143. MAYO CLIN,ROCHESTER,MN. UNIV ALBERTA,EDMONTON,AB,CANADA. MED COLL WISCONSIN,MILWAUKEE,WI 53226. ALBERT EINSTEIN MED CTR,PHILADELPHIA,PA. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. AMER COLL RADIOL,PHILADELPHIA,PA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 488 EP 488 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700519 ER PT J AU Oliva, F Ferry, JA Young, RH Prat, J Srigley, J Scully, RE AF Oliva, F Ferry, JA Young, RH Prat, J Srigley, J Scully, RE TI Granulocytic sarcoma of the female genital tract. A clinicopathologic study of 19 cases. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 HARVARD UNIV,SCH MED,BOSTON,MA. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HOSP STA CREU & ST PAU,BARCELONA,SPAIN. SUNNYBROOK HLTH SCI CTR,TORONTO,ON M4N 3M5,CANADA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 550 EP 550 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700581 ER PT J AU Oliva, E Sgroi, D Scully, RE AF Oliva, E Sgroi, D Scully, RE TI Expression of Bcl-2 and estrogen receptors in ovarian neoplasms. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 551 EP 551 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700582 ER PT J AU Dorfman, DM Schultze, JL Shahsafaei, A Gribben, JG Freeman, GJ Pinkus, GS Nadler, LM AF Dorfman, DM Schultze, JL Shahsafaei, A Gribben, JG Freeman, GJ Pinkus, GS Nadler, LM TI Follicular lymphomas express low but detectable levels of B7 costimulatory molecules: A possible mechanism for tumor cell immune tolerance SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DANA FARBER CANC INST,BOSTON,MA 02115. RI Schultze, Joachim/D-7794-2011 OI Schultze, Joachim/0000-0003-2812-9853 NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 640 EP 640 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700671 ER PT J AU Nguyen, PL Olszak, I Harris, NL Preffer, FI AF Nguyen, PL Olszak, I Harris, NL Preffer, FI TI Staining for myeloperoxidase (MPO) in acute leukemias: A comparison of flow cytometry and enzyme cytochemistry. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 689 EP 689 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700720 ER PT J AU Nguyen, PL Ferry, JA Harris, NL AF Nguyen, PL Ferry, JA Harris, NL TI Progressive transformation of germinal centers (PTGC) and nodular lymphocyte predominant Hodgkin's disease (NLPHD): A comparative immunohistochemical study. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 690 EP 690 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700721 ER PT J AU Pinkus, GS Pinkus, JL Matsumura, F YamashiroMatsumura, S Langhoff, E Mosialos, G Said, JW AF Pinkus, GS Pinkus, JL Matsumura, F YamashiroMatsumura, S Langhoff, E Mosialos, G Said, JW TI Fascin, a sensitive new marker MR Reed-Sternberg cells of Hodgkin's disease - Evidence for a dendritic or B cell origin? SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 BRIGHAM & WOMENS HOSP,BOSTON,MA 02115. RUTGERS STATE UNIV,PISCATAWAY,NJ. DANA FARBER CANC INST,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BOSTON,MA. CEDARS SINAI MED CTR,LOS ANGELES,CA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 697 EP 697 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700728 ER PT J AU Savilo, E Campo, E Harris, NL Mollejo, M Piris, MA AF Savilo, E Campo, E Harris, NL Mollejo, M Piris, MA TI Cyclin D1 expression in splenic marginal zone lymphoma SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HOSP CLIN BARCELONA,BARCELONA,SPAIN. HOSP VIRGEN SALUD,TOLEDO,SPAIN. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 710 EP 710 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700741 ER PT J AU vanGorder, M Bhan, AK GraemeCook, F AF vanGorder, M Bhan, AK GraemeCook, F TI Cytomegalovirus - The diagnostic role of immunohistochemistry SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 771 EP 771 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700802 ER PT J AU delaMonte, SM Wands, JR AF delaMonte, SM Wands, JR TI Probable distinct pathogenic mechanisms of subacute encephalitis and subacute encephalopathy in AIDS. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 815 EP 815 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700846 ER PT J AU delaMonte, SM Wands, JR AF delaMonte, SM Wands, JR TI Increased apoptosis with p53 and Fas antigen up-regulation in Alzheimer's disease (AD). SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 816 EP 816 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700847 ER PT J AU delaMonte, SM Sohn, YK Hutchins, GM Wands, JR AF delaMonte, SM Sohn, YK Hutchins, GM Wands, JR TI Abnormal glial cell gene expression is an early and consistent feature of Alzheimer's disease. SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 JOHNS HOPKINS MED INST,BALTIMORE,MD 21205. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 817 EP 817 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700848 ER PT J AU Matsubara, O Miyake, S Shibata, H Kagata, Y Mark, EJ AF Matsubara, O Miyake, S Shibata, H Kagata, Y Mark, EJ TI Hamman-Rich syndrome variant in dermatomyositis and polymyositis: Clinicopathologic assessment of 12 autopsy SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 NATL DEF MED COLL,TOKOROZAWA,SAITAMA,JAPAN. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 938 EP 938 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700969 ER PT J AU Borczuk, AC Borczuk, PM Acquaviva, R Jones, JG AF Borczuk, AC Borczuk, PM Acquaviva, R Jones, JG TI Prediction of axillary lymph node metastases in breast cancer patients using an artificial neural network SO LABORATORY INVESTIGATION LA English DT Meeting Abstract C1 ALBERT EINSTEIN COLL MED,NEW YORK,NY. MASSACHUSETTS GEN HOSP,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0023-6837 J9 LAB INVEST JI Lab. Invest. PD JAN PY 1996 VL 74 IS 1 BP 967 EP 967 PG 1 WC Medicine, Research & Experimental; Pathology SC Research & Experimental Medicine; Pathology GA TT757 UT WOS:A1996TT75700998 ER PT J AU Wiegand, DA Ojemann, RG Fickel, V AF Wiegand, DA Ojemann, RG Fickel, V TI Surgical treatment of acoustic neuroma (vestibular schwannoma) in the United States: Report from the acoustic neuroma registry SO LARYNGOSCOPE LA English DT Article ID PATIENTS PERSPECTIVE; EXCISION AB In 1989, the Acoustic Neuroma Association established a multisurgeon, multi-institutional registry to collect data related to the treatment of patients with acoustic neuroma. This report analyzes information from the 1579 surgically treated patients who were entered in the registry between January 1, 1989, and February 28, 1994. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT SURG,NEUROSURG SERV,BOSTON,MA. ACOUST NEUROMA REGISTRY,CARLISLE,PA. NR 15 TC 104 Z9 106 U1 0 U2 4 PU LARYNGOSCOPE CO PI ST LOUIS PA 10 S BROADWAY 14TH FLOOR, ST LOUIS, MO 63102-1741 SN 0023-852X J9 LARYNGOSCOPE JI Laryngoscope PD JAN PY 1996 VL 106 IS 1 BP 58 EP 66 DI 10.1097/00005537-199601000-00012 PN 1 PG 9 WC Medicine, Research & Experimental; Otorhinolaryngology SC Research & Experimental Medicine; Otorhinolaryngology GA TN814 UT WOS:A1996TN81400012 PM 8544629 ER PT B AU Pogue, BW Redmond, RW Hasan, T AF Pogue, BW Redmond, RW Hasan, T BE Jacques, SL Katzir, A TI A study of dosimetry for pulsed-laser photodynamic therapy SO LASER-TISSUE INTERACTION VII, PROCEEDINGS OF SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Laser-Tissue Interaction VII CY JAN 29-FEB 01, 1996 CL SAN JOSE, CA SP Soc Photo Opt Instrumentat Engineers C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. NR 0 TC 6 Z9 6 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-2055-7 J9 P SOC PHOTO-OPT INS PY 1996 VL 2681 BP 130 EP 139 DI 10.1117/12.239565 PG 10 WC Engineering, Biomedical; Optics SC Engineering; Optics GA BF67R UT WOS:A1996BF67R00017 ER PT B AU Flotte, TJ Lee, S Zhang, H McAuliffe, D Taitelbaum, E Doukas, A AF Flotte, TJ Lee, S Zhang, H McAuliffe, D Taitelbaum, E Doukas, A BE Jacques, SL Katzir, A TI Laser-induced stress transients: Aqueous pores of membranes SO LASER-TISSUE INTERACTION VII, PROCEEDINGS OF SE PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) LA English DT Proceedings Paper CT Conference on Laser-Tissue Interaction VII CY JAN 29-FEB 01, 1996 CL SAN JOSE, CA SP Soc Photo Opt Instrumentat Engineers DE laser; pressure; gene therapy; cancer therapy C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPIE - INT SOC OPTICAL ENGINEERING PI BELLINGHAM PA PO BOX 10, BELLINGHAM, WA 98227-0010 BN 0-8194-2055-7 J9 P SOC PHOTO-OPT INS PY 1996 VL 2681 BP 160 EP 166 DI 10.1117/12.239569 PG 7 WC Engineering, Biomedical; Optics SC Engineering; Optics GA BF67R UT WOS:A1996BF67R00020 ER PT J AU Stewart, RB Benbrahim, A LaMuraglia, GM Rosenberg, M LItalien, GJ Abbott, WM Kung, RTV AF Stewart, RB Benbrahim, A LaMuraglia, GM Rosenberg, M LItalien, GJ Abbott, WM Kung, RTV TI Laser assisted vascular welding with real time temperature control SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE diode laser; feedback loop; infrared thermometry; real time control; thermal weld ID ANASTOMOSIS; REPAIR AB Background and Objective: Previous studies in laser assisted vascular welding have been limited by the lack of a reliable end point for tissue fusion. As a means of improving the reproductibility of laser assisted repairs, a system incorporating real time temperature monitoring and closed loop feedback was used. Study Design/Materials and Methods: The system consisted of a direct view infrared thermometer for monitoring the laser heated spot, a 1.9 mu m diode laser, and a microprocessor for data acquisition and feedback control of the laser power to maintain a constant tissue temperature. Rat aortas were welded under constant surface temperature conditions. Results: In vivo temperature stability of +/-2 degrees C was achieved over a temperature range of 70-90 degrees C pertinent to welding small vessels. When welds were completed using the feedback system to maintain the tissue temperature at 80 degrees C, the acute success rate was 100% and the burst pressure was 290 +/- 70 mmHg. Conclusion: These studies demonstrate that the use of real time monitoring and feedback control results in improved consistency for vascular tissue welding. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DIV VASC SURG,BOSTON,MA 02114. RP Stewart, RB (reprint author), ABIOMED INC,24 CHERRY HILL DR,DANVERS,MA 01923, USA. FU NHLBI NIH HHS [HL-02583, R44-HL45390] NR 14 TC 38 Z9 38 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 19 IS 1 BP 9 EP 16 DI 10.1002/(SICI)1096-9101(1996)19:1<9::AID-LSM2>3.0.CO;2-W PG 8 WC Dermatology; Surgery SC Dermatology; Surgery GA UZ276 UT WOS:A1996UZ27600003 PM 8836991 ER PT J AU Lewandrowski, KU Lorente, C Schomacker, KT Flotte, TJ Wilkes, JW Deutsch, TF AF Lewandrowski, KU Lorente, C Schomacker, KT Flotte, TJ Wilkes, JW Deutsch, TF TI Use of the Er:YAG laser for improved plating in maxillofacial surgery: Comparison of bone healing in laser and drill osteotomies SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE Er:YAG laser; facial bones; laser osteotomy; healing ID MICROVASCULAR ANASTOMOSES; CONTINUOUS-WAVE; CARBON-DIOXIDE; ABLATION; RADIATION; DEFECTS; ERBIUM; TISSUE; GRAFT AB Background and Objective: Surgical reconstruction of bony defects in the maxillofacial region involves fixation of bony fragments with mini and micro plates. Bone stabilization during hole drilling is often challenging due to the need to apply pressure when using a conventional mechanical Hall drill. In addition, fragmentation of the fragile bones may occur and complicate the reconstruction. The pulsed Er:YAG laser offers an attractive alternative drilling modality because it does not require physical contact with the bone in order to drill holes, cuts bone with minimal thermal damage, and allows precise control of bone cutting. The objective of this study was to investigate the pulsed Er:YAG laser as an alternative to the mechanical bur by comparing bone healing using both modalities. Study Design/Materials and Methods: Bone healing in an inferior border defect of the rat mandible was examined using either an Er:YAG laser or a mechanical bur for drilling. The healing of osteotomies in facial bones and of screw holes for plate stabilization of free bone fragments was studied. Results: All defects healed by 4 weeks postoperatively. Histologic evaluation demonstrated no difference in the amount of newly formed woven bone at the osteotomy site or screw holes made by either the laser or the drill. The extent of thermal damage at the osteotomy sites was comparable in laser and mechanically cut bone fragments. Conclusions: On the basis of this study we suggest that the Er:YAG laser can be used clinically in thin, fragile bones in the maxillofacial region. (C) 1996 Wiley-Liss, Inc. C1 MASSACHUSETTS GEN HOSP,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,ORTHOPAED RES LAB,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT ORAL & MAXILLOFACIAL SURG,BOSTON,MA 02114. NR 24 TC 61 Z9 63 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 19 IS 1 BP 40 EP 45 DI 10.1002/(SICI)1096-9101(1996)19:1<40::AID-LSM6>3.0.CO;2-Q PG 6 WC Dermatology; Surgery SC Dermatology; Surgery GA UZ276 UT WOS:A1996UZ27600007 PM 8836995 ER PT J AU Domankevitz, Y McMillan, K Nishioka, NS AF Domankevitz, Y McMillan, K Nishioka, NS TI Characterization of tissue ablation with a continuous wave holmium laser SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE laser ablation; thermal damage; mass loss ID PULSED HOLMIUM; YAG LASER; INJURY AB Background and Objective: The pulsed holmium laser is a promising tool for tissue ablation but possesses some limitations. For example, it is capable of producing significant mechanical damage in certain tissues in the form of fissures and fractures. Because longer pulse durations should reduce mechanical damage, this study examined the tissue effects produced by a prototype continuous wave holmium laser. Study Design/Materials and Methods: A prototype liquid nitrogen cooled holmium operating at 2.12 mu m was used. The heat of ablation and ablation threshold were determined; using a mass loss technique, Fresh pig skin was irradiated in air or under saline, and prepared for histologic analysis. Hemostasis was qualitatively assessed in vivo during incisions made in the skin, Liver, and small intestine. Results: Threshold radiant exposure and heat of ablation were calculated from the mass loss measurements to be 191 J/cm(2) with a 95% confidence interval of -80-440 J/cm(2). Residual thermal damage in skin ranged from 390 to 690 mu m in saline and from 390 to 490 mu m in air. Excellent hemostasis was achieved in all incisions. Conclusion: Using appropriate irradiation parameters, the continuous wave holmium laser produces tissue effects suitable for a general use surgical instrument. In addition, the laser source could become compact and inexpensive when diode-pumped and direct diode devices become available. (C) 1996 Wiley-Liss, Inc. C1 MASSACHUSETTS GEN HOSP,MED SERV,GASTROINTESTINAL UNIT,BOSTON,MA 02114. HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,WELLMAN LABS PHOTOMED,BOSTON,MA. CANDELA LASER CORP,WAYLAND,MA. FU NINDS NIH HHS [1R43NS32198-01] NR 21 TC 6 Z9 7 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 19 IS 1 BP 97 EP 102 DI 10.1002/(SICI)1096-9101(1996)19:1<97::AID-LSM11>3.0.CO;2-8 PG 6 WC Dermatology; Surgery SC Dermatology; Surgery GA UZ276 UT WOS:A1996UZ27600012 PM 8837000 ER PT J AU Ross, EV Domankevitz, Y Skrobal, M Anderson, RR AF Ross, EV Domankevitz, Y Skrobal, M Anderson, RR TI Effects of CO2 laser pulse duration in ablation and residual thermal damage: Implications for skin resurfacing SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE irradiance; mass loss; radiant exposure ID CO2-LASER TISSUE ABLATION AB Background and Objectives: Resurfacing with the CO2 laser is rapidly gaining acceptance for skin rejuvenation. Advances in CO2 laser and scanning technology allow for precise tissue removal with minimal thermal damage. High energy CO2 laser pulses have been widely used effectively to smooth the surface of facial skin; however, pulse duration effects on ablation and thermal damage have not been systematically studied over the millisecond region (0.25-10 ms). Study Design/Materials and Methods: This study characterizes the ablation threshold, heat of ablation, and residual thermal damage in skin resulting from CO2 laser pulses with a Gaussian beam profile. Mass loss from fresh pig skin was measured with an analytical balance, and residual thermal damage was determined through histology. Results: Pulse durations >1 ms were associated with higher ablation thresholds and localized increased thermal damage. Conclusions: Our results show that although pulse duration is an important determinant in ablation and thermal damage, irradiance is more critical as an independent parameter in predicting the effects of CO2 laser pulses. (C) 1996 Wiley-Liss, Inc. RP Ross, EV (reprint author), MASSACHUSETTS GEN HOSP,WELLMAN LABS PHOTOMED,DEPT DERMATOL,BHX-630,55 FRUIT ST,BOSTON,MA 02114, USA. NR 13 TC 82 Z9 83 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 19 IS 2 BP 123 EP 129 PG 7 WC Dermatology; Surgery SC Dermatology; Surgery GA VK458 UT WOS:A1996VK45800001 PM 8887913 ER PT J AU Douki, T Lee, S Dorey, K Flotte, TJ Deutsch, TF Doukas, AG AF Douki, T Lee, S Dorey, K Flotte, TJ Deutsch, TF Doukas, AG TI Stress-wave-induced injury to retinal pigment epithelium cells in vitro SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE shock waves; stress transient; cell killing; retinal pigment epithelium ID LITHOTRIPTER SHOCK-WAVES; PICOSECOND OPTICAL-BREAKDOWN; EXCIMER-LASER; CANCER CELLS; INVITRO; CAVITATION; DAMAGE; PERMEABILITY; IRRADIATION; L1210-CELLS AB Background and Objective: To determine the survival of in vitro retinal pigment epithelium (RPE) cells subjected to laser-generated stress transients (shock waves) and compare it to that of other cell lines. Study Design/Materials and Methods: Normal and transformed human retinal pigment epithelium cell lines were used. The cells were imbedded in a gel to prevent motion and cavitation and located in a thin layer at the bottom of a pipette tube closed at one end by a polyimide film. Stress transients were generated by pulsed excimer laser (193 nn and 248 nm wavelength) ablation of the polyimide film. Cell survival, compared to that of unirradiated cells, was assessed by counting surviving cells. The stress was varied from 300 to 740 bars and the number of shock wave pulses applied varied from 5 to 150. Results: Cell survival decreased sharply at the higher stresses but some cells always survived. The lowest survival rate was 50%. Increasing the number of shock wave pulses did not increase cell killing after 20 pulses, demonstrating a saturation effect. In contrast to the transformed cell Line, normal cells could not be killed at the highest stress available to us. Conclusion: The susceptibility of RPE cells to damage by stress waves varies with cell. line. Transformed retinal pigment epithelium cells are more susceptible than normal ones. Saturation of the damage versus number of pulses is observed and a threshold-like behavior for cell killing versus stress is found. Because at least 50% of the cells survived, normal cell growth can serve to replenish damaged cells. (C) 1996 Wiley-Liss, Inc. C1 MASSACHUSETTS GEN HOSP,WELLMAN LABS PHOTOMED,BOSTON,MA 02114. HARVARD UNIV,SCH MED,BOSTON,MA. SCHEPENS EYE RES INST,BOSTON,MA 02114. FU PHS HHS [R01-8121] NR 33 TC 12 Z9 13 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 19 IS 3 BP 249 EP 259 PG 11 WC Dermatology; Surgery SC Dermatology; Surgery GA VR669 UT WOS:A1996VR66900001 PM 8923421 ER PT J AU Berger, JW Bochow, TW Talamo, JH DAmico, DJ AF Berger, JW Bochow, TW Talamo, JH DAmico, DJ TI Measurement and modeling of thermal transients during Er:YAG laser irradiation of vitreous SO LASERS IN SURGERY AND MEDICINE LA English DT Article; Proceedings Paper CT 1995 Meeting of the American-Society-for-Laser-Medicine-and-Surgery CY 1995 CL SAN DIEGO, CA SP Amer Soc Laser Med & Surg DE erbium; heat transfer; infrared; modeling; vitreous ID CARBON-DIOXIDE LASER; RABBIT EYES; ERBIUM; ABLATION; SURGERY; MEMBRANES; VITRECTOMY; YSGG; LENS AB Background and Objective: We investigated the transient thermal behavior of vitreous in order to understand the local thermal effects of laser output, and to predict the potential for unintentional injury during Er:YAG laser vitreoretinal surgery. Study Design/Materials and Methods: The output of a free-running Er:YAG laser (2.94 mu m, 300 mu s FWHM) was delivered through a fiberoptic and applied to en bloc samples of bovine vitreous. Temperature was measured with ultrafine thermocouples. Results: For 6 mJ pulse energy at 10 Hz, a temperature rise of 20 degrees C is measured 500 mu m from the laser tip. The temperature rise is localized with a rapid fall-off greater than I mm from the energy source. At constant time-averaged laser power, the temperature profile is independent of repetition rate. Our finite-difference model generates results qualitatively consistent with measured data and allows for investigation of the influence of thermophysical parameters on heat transfer. Conclusion: Thermal injury to ocular structures should be Limited during intravitreal application of Er:YAG laser energy. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,MASSACHUSETTS EYE & EAR INFIRM,SCH MED,DEPT OPHTHALMOL,LASER RES LAB,BOSTON,MA 02114. NR 27 TC 14 Z9 15 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 19 IS 4 BP 388 EP 396 DI 10.1002/(SICI)1096-9101(1996)19:4<388::AID-LSM2>3.0.CO;2-O PG 9 WC Dermatology; Surgery SC Dermatology; Surgery GA VZ838 UT WOS:A1996VZ83800002 PM 8982997 ER PT J AU vanLeeuwen, RL Dekker, SK Byers, HR Vermeer, BJ Grevelink, JM AF vanLeeuwen, RL Dekker, SK Byers, HR Vermeer, BJ Grevelink, JM TI Modulation of alpha 4 beta 1 and alpha 5 beta 1 integrin expression: Heterogeneous effects of Q-switched ruby, Nd:YAG, and alexandrite lasers on melanoma cells in vitro SO LASERS IN SURGERY AND MEDICINE LA English DT Article DE laser; integrin; melanoma; migration; attachment; fibronectin ID HEAT-SHOCK RESPONSE; MIGRATION; ADHESION; RADIATION; LAMININ AB Background and Objective: Integrins of the beta 1 family are cellular adhesion molecules that play an important role in cell attachment and migration by interacting with extracellular matrix molecules. Agents such as hormones, cytokines, and ultraviolet radiation have all been shown to have an integrin modulating potential. The present study indicates that radiation of Q-switched lasers is also able to induce transient changes in integrin expression levels on human melanoma cells in vitro. Study Design/Materials and Methods: Radiation from Q-switched Ruby (694 nm), Alexandrite (755 nm), and Nd:YAG laser (1,064 nm) with fluences comparable to those that are generally used in treating dermatologic lesions were used to irradiate a subconfluent layer of human melanoma cells. After fixed time intervals, the cells were harvested either to analyse the integrin expression by flow cytometry or to investigate changes in cell attachment, spreading, and migration. Results: It was established that all three types of laser were able to cause a significant downregulation of both the alpha 4 and the common beta 1 integrin subunit. The Alexandrite and Ruby lasers also induced a decrease in alpha 5 expression; however, the cells treated with the Nd:YAG laser showed a marked upregulation of the alpha 5 subunit. The expression of the other beta 1 integrin subunits was shown to be unaltered after laser treatment. Downregulation of the alpha 4 upregulation of the alpha 5 integrin subunit expression resulted in, respectively, decreased and increased attachment and spreading on fibronectin, the extracellular matrix ligand for both the alpha 4 beta 1 and alpha 5 beta 1 integrins. Marked upregulation of the alpha 5 subunit also resulted in a higher migration rate. Conclusion: Taken together, these results show that nonlethal doses of Q-switched laser radiation are able to induce changes in cellular behavior in vitro by modulating the integrin expression pattern. (C) 1996 Wiley-Liss, Inc. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,CTR DERMATOL LASER,DIV DERMATOPATHOL,BOSTON,MA 02114. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT PATHOL,DIV DERMATOPATHOL,BOSTON,MA 02114. LEIDEN UNIV,DEPT DERMATOL,LEIDEN,NETHERLANDS. NR 19 TC 17 Z9 17 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC 605 THIRD AVE, NEW YORK, NY 10158-0012 SN 0196-8092 J9 LASER SURG MED JI Lasers Surg. Med. PY 1996 VL 18 IS 1 BP 63 EP 71 PG 9 WC Dermatology; Surgery SC Dermatology; Surgery GA TM763 UT WOS:A1996TM76300008 PM 8850467 ER PT J AU Paietta, E Andersen, J Wiernik, PH AF Paietta, E Andersen, J Wiernik, PH TI A new approach to analyzing the utility of immunophenotyping for predicting clinical outcome in acute leukemia SO LEUKEMIA LA English DT Editorial Material ID CLASSIFICATION; LYMPHOMA C1 ALBERT EINSTEIN CANC CTR,BRONX,NY. DANA FARBER CANC INST,DIV BIOSTAT,BOSTON,MA 02115. EASTERN COOPERAT ONCOL GRP,BOSTON,MA. RP Paietta, E (reprint author), MONTEFIORE MED CTR,DEPT ONCOL,111 E 210TH ST,BRONX,NY 10467, USA. FU NCI NIH HHS [CA21115, CA14958, CA23318] NR 30 TC 15 Z9 15 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE, HAMPSHIRE, ENGLAND RG21 6XS SN 0887-6924 J9 LEUKEMIA JI Leukemia PD JAN PY 1996 VL 10 IS 1 BP 1 EP 4 PG 4 WC Oncology; Hematology SC Oncology; Hematology GA TT760 UT WOS:A1996TT76000001 PM 8558912 ER PT J AU Duerinckx, A Atkinson, D Klitzner, TS Perloff, J Drinkwater, D Laks, H AF Duerinckx, A Atkinson, D Klitzner, TS Perloff, J Drinkwater, D Laks, H TI MR imaging of surgical complications of systemic-to-pulmonary artery shunts SO MAGNETIC RESONANCE IMAGING LA English DT Article DE magnetic resonance imaging; MRI; cardiac imaging; breathhold MRI; congenital heart disease; surgery; shunts; aneurysm; shunt leak ID BLALOCK-TAUSSIG SHUNT; NUCLEAR-MAGNETIC-RESONANCE; CORONARY-ARTERIES; ECHOCARDIOGRAPHY; ANGIOGRAPHY; ATRESIA; AORTA; HEART; ECHO AB Patients with a systemic-to-pulmonary artery shunt and positive findings on traditional imaging modalities such as chest X-ray, echocardiography, or cardiac angiography often can benefit from additional noninvasive imaging with magnetic resonance imaging (MRI). Diagnostic dilemmas encountered include: pseudoaneurysms, contained fluid collection (seroma) surrounding a shunt, and stenosis of the shunt anastomoses. MRI studies using traditional cardiac-triggered spin-echo (SE) imaging and the newer breathhold MRI studies with k-space segmented gradient-recalled echo (GRE) imaging can greatly help resolve diagnostic dilemmas, By combining different MR imaging techniques it becomes possible to clearly distinguish between pseudoaneurysms and seroma, to exclude an active leak and to sometimes visualize the distal anastomosis with more precision than conventional angiography, MRI is often able to add information needed for clinical decision making prior to surgical repair. Copyright (C) 1996 Elsevier Science Inc. RP Duerinckx, A (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,SERV RADIOL,MAIL ROUTE W114,MRI,BLD 507,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. RI Atkinson, Dennis/N-5238-2015 OI Atkinson, Dennis/0000-0001-7393-7505 NR 35 TC 7 Z9 7 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND OX5 1GB SN 0730-725X J9 MAGN RESON IMAGING JI Magn. Reson. Imaging PY 1996 VL 14 IS 9 BP 1099 EP 1105 DI 10.1016/S0730-725X(96)00112-9 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA WG847 UT WOS:A1996WG84700013 PM 9071002 ER PT J AU Toussaint, JF Kwong, KK MKparu, F Weisskoff, RM LaRaia, PJ Kantor, HL AF Toussaint, JF Kwong, KK MKparu, F Weisskoff, RM LaRaia, PJ Kantor, HL TI Perfusion changes in human skeletal muscle during reactive hyperemia measured by echo-planar imaging SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE muscle; perfusion; human; NMR; T-1 ID BLOOD-FLOW; EXERCISE; WATER; VASODILATION; STIMULATION; CONTRAST; SPACES; NMR AB Muscle performance is markedly influenced by tissue perfusion, Techniques that allow quantification of microvascular flow are limited by the use of ionizing radiation. In this investigation, we apply an NMR model previously developed by Detre et al. to the measurement of human muscle perfusion during reactive hyperemia, We compare our results with conventional plethysmography adapted to NMR, Using echo-planat imaging, T-1 and T-2 were measured in 14 subjects during rest, ischemia, and reactive hyperemia, Mean leg muscle T-1 in healthy volunteers is 850 ms at rest and 834 ms at reperfusion, leading to a calculated reactive hyperemia flow increase (T-1 flow) of 103 +/- 40 ml/100 ml/min. T-1 flows correlate well with NMR-plethysmography values. Changes in T-2, which are sensitive to both deoxyhemoglobin content and vessel diameter variations, are also correlated with perfusion measurements, T-1 changes allow quantification of regional perfusion in human muscle during reactive hyperemia. C1 MASSACHUSETTS GEN HOSP,NMR CTR,BOSTON,MA 02114. SPAULDING REHABIL HOSP,BOSTON,MA. RP Toussaint, JF (reprint author), MASSACHUSETTS GEN HOSP,CARDIAC UNIT,JACKSON 1410,32 FRUIT ST,BOSTON,MA 02114, USA. FU NHLBI NIH HHS [R01-HL39371] NR 47 TC 73 Z9 73 U1 0 U2 1 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD JAN PY 1996 VL 35 IS 1 BP 62 EP 69 DI 10.1002/mrm.1910350109 PG 8 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TN368 UT WOS:A1996TN36800008 PM 8771023 ER PT J AU Kao, YH Sorenson, JA Winkler, SS AF Kao, YH Sorenson, JA Winkler, SS TI MR image segmentation using vector decomposition and probability techniques: A general model and its application to dual-echo images SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE segmentation; volume measurement; fractional volume; image processing ID MAGNETIC-RESONANCE IMAGES; CEREBROSPINAL-FLUID VOLUMES; FAISE METHOD; MULTISPECTRAL ANALYSIS; GRAY-MATTER; NOISE; SEQUENCES; BRAIN; CONNECTIVITY; ALGORITHM AB A general model is developed for segmenting magnetic resonance images using vector decomposition and probability techniques. Each voxel is assigned fractional volumes of q tissues from p differently weighted images (q less than or equal to p + 1) in the presence of partial-volume mixing, random noise, and other tissues. Compared with the eigenimage method, fewer differently weighted images are needed for segmenting the q tissues, and the contrast-to-noise ratio in the calculated fractional volumes is improved. The model can produce composite tissue-type images similar to that of the probability methods, by comparing the fractional volumes assigned to different tissues on each voxel. A three-tissue (p = 2, q = 3) model is illustrated for segmenting three tissues from dual-echo images, It provides statistical analysis to the algebraic method. A three-compartment phantom is segmented for validation. Two clinical examples are presented. C1 UNIV WISCONSIN,DEPT PHYS,MADISON,WI 53706. UNIV WISCONSIN,DEPT MED PHYS,MADISON,WI 53706. UNIV WISCONSIN,DEPT RADIOL,MADISON,WI 53706. WILLIAM S MIDDLETON MEM VET ADM MED CTR,SERV RADIOL,MADISON,NJ. RP Kao, YH (reprint author), DUKE UNIV,MED CTR,DEPT RADIOL,ROOM 129,BRYAN RES BLDG,BOX 3302,DURHAM,NC 27710, USA. NR 46 TC 13 Z9 13 U1 0 U2 0 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0740-3194 J9 MAGNET RESON MED JI Magn.Reson.Med. PD JAN PY 1996 VL 35 IS 1 BP 114 EP 125 DI 10.1002/mrm.1910350115 PG 12 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TN368 UT WOS:A1996TN36800014 PM 8771029 ER PT J AU Larsson, M Tissir, F Walter, L Gunther, E Jacob, H KlingaLevan, K Levan, G AF Larsson, M Tissir, F Walter, L Gunther, E Jacob, H KlingaLevan, K Levan, G TI Mapping of the rat ribosomal protein S18 gene (Rps 18) to chromosome 20p12 SO MAMMALIAN GENOME LA English DT Article C1 FREE UNIV BRUSSELS,DEPT MOLEC BIOL,B-1640 RHODE ST GENESE,BELGIUM. UNIV GOTTINGEN,DEPT IMMUNOGENET,GOTTINGEN,GERMANY. MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,BOSTON,MA. RP Larsson, M (reprint author), GOTHENBURG UNIV,DEPT GENET,S-41390 GOTHENBURG,SWEDEN. NR 8 TC 9 Z9 9 U1 0 U2 1 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD JAN PY 1996 VL 7 IS 1 BP 90 EP 90 DI 10.1007/s003359900024 PG 1 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA TQ967 UT WOS:A1996TQ96700025 PM 8903742 ER PT J AU Burgess, JF Wilson, PW AF Burgess, JF Wilson, PW TI Hospital ownership and technical inefficiency SO MANAGEMENT SCIENCE LA English DT Article DE DEA; distance functions; efficiency; contract failure; ownership structure ID EFFICIENCY; INCENTIVES; MODEL; FIRMS AB The theoretical industrial organization literature cites varying factors which might influence the degree of technical efficiency achieved under different ownership structures in the US hospital industry. Unfortunately, this literature offers no consensus regarding the net direction and magnitude of these various effects. This study analyzes the four types of ownership structure in the US hospital industry-private nonprofit, private for-profit, federal, and state and local government. Distance functions are used to measure technical efficiency of hospitals producing multiple outputs relative to other hospitals in the sample, allowing comparisons among the different ownership types. C1 UNIV TEXAS,DEPT ECON,AUSTIN,TX 78712. RP Burgess, JF (reprint author), US DEPT VET AFFAIRS,MANAGEMENT SCI GRP 518MSG,BEDFORD,MA 01730, USA. NR 38 TC 40 Z9 40 U1 3 U2 13 PU INST OPERATIONS RESEARCH MANAGEMENT SCIENCES PI LINTHICUM HTS PA 901 ELKRIDGE LANDING RD, STE 400, LINTHICUM HTS, MD 21090-2909 SN 0025-1909 J9 MANAGE SCI JI Manage. Sci. PD JAN PY 1996 VL 42 IS 1 BP 110 EP 123 DI 10.1287/mnsc.42.1.110 PG 14 WC Management; Operations Research & Management Science SC Business & Economics; Operations Research & Management Science GA UE441 UT WOS:A1996UE44100008 ER PT J AU Beatty, J Biggs, PJ Gall, K Okunieff, P Pardo, FS Harte, KJ Dalterio, MJ Sliski, AP AF Beatty, J Biggs, PJ Gall, K Okunieff, P Pardo, FS Harte, KJ Dalterio, MJ Sliski, AP TI A new miniature x-ray device for interstitial radiosurgery: Dosimetry SO MEDICAL PHYSICS LA English DT Article DE stereotactic radiosurgery; dosimetry; low-energy x rays AB A miniature, battery operated 40 kV x-ray device has been developed for the interstitial treatment of small tumors (<3 cm diam) in humans. X rays are emitted from the tip of a 10 cm long, 3 mm diameter probe that is stereotactically inserted into the tumor. The beam, characterized by half-value layer (HVL), spectrum analysis, and isodose contours, behaves essentially as a point isotropic source with an effective energy of 20 keV at a depth of 10 mm in water. The absolute output from the device was measured using a parallel plate ionization chamber, modified with a platinum aperture. The dose rate in water determined from these chamber measurements was found to be nominally 150 cGy/min at a distance of 10 mm for a beam current of 40 mu A and voltage of 40 kV. The dose in water falls off approximately as the third power of the distance. To date, 14 patients have been treated with this device in a phase I clinical trial. (C) 1996 American Association of Physicists in Medicine. C1 PHOTOELECTRON CORP,WALTHAM,MA 02154. RP Beatty, J (reprint author), HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT RADIAT ONCOL,DIV RADIAT BIOPHYS,BOSTON,MA 02114, USA. NR 9 TC 65 Z9 66 U1 0 U2 3 PU AMER INST PHYSICS PI WOODBURY PA CIRCULATION FULFILLMENT DIV, 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2999 SN 0094-2405 J9 MED PHYS JI Med. Phys. PD JAN PY 1996 VL 23 IS 1 BP 53 EP 62 DI 10.1118/1.597791 PG 10 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA TR499 UT WOS:A1996TR49900007 PM 8700033 ER PT S AU Langley, RGB Sober, AJ AF Langley, RGB Sober, AJ BE Hori, Y Hearing, VJ Nakayama, J TI New techniques in the early diagnosis of melanoma SO MELANOGENESIS AND MALIGNANT MELANOMA: BIOCHEMISTRY, CELL BIOLOGY, MOLECULAR BIOLOGY, PATHOPHYSIOLOGY, DIAGNOSIS AND TREATMENT SE INTERNATIONAL CONGRESS SERIES LA English DT Proceedings Paper CT International Symposium on Melanogenesis and Malignant Melanoma CY DEC 04-06, 1995 CL FUKUOKA, JAPAN SP Minist Educ Sci & Culture, Japan DE early diagnosis; epiluminescence; image analysis; melanoma C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,DEPT DERMATOL,BOSTON,MA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE PUBL B V PI AMSTERDAM PA SARA BURGERHARTSTRAAT 25, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0531-5131 BN 0-444-82209-7 J9 INT CONGR SER PY 1996 VL 1096 BP 233 EP 245 PG 13 WC Oncology; Cell Biology; Dermatology SC Oncology; Cell Biology; Dermatology GA BG27L UT WOS:A1996BG27L00024 ER PT J AU Cantiello, HF Prat, AG AF Cantiello, HF Prat, AG TI Role of actin filament organization in ion channel activity and cell volume regulation SO MEMBRANE PROTEIN-CYTOSKELETON INTERACTIONS SE CURRENT TOPICS IN MEMBRANES LA English DT Review ID EPITHELIAL NA+ CHANNELS; BINDING-PROTEIN; F-ACTIN; PHASE-TRANSITIONS; PLASMA-MEMBRANES; SODIUM-CHANNEL; CYTOCHALASIN-D; KIDNEY-CELLS; CYTOSKELETON; IDENTIFICATION C1 HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115. RP Cantiello, HF (reprint author), MASSACHUSETTS GEN HOSP EAST,RENAL UNIT,CHARLESTOWN,MA 02129, USA. NR 62 TC 6 Z9 6 U1 0 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 J9 CURR TOP MEMBR PY 1996 VL 43 BP 373 EP 396 PG 24 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA BG40D UT WOS:A1996BG40D00017 ER PT J AU Morgan, BA Fekete, DM AF Morgan, BA Fekete, DM TI Manipulating gene expression with replication-competent retroviruses SO METHODS IN CELL BIOLOGY, VOL 51 SE METHODS IN CELL BIOLOGY LA English DT Review ID ROUS-SARCOMA VIRUS; VECTORS; INFECTION; STRAIN; EMBRYO; DNA C1 MASSACHUSETTS GEN HOSP,CHARLESTOWN,MA 02129. BOSTON COLL,DEPT BIOL,CHESTNUT HILL,MA 02167. RP Morgan, BA (reprint author), HARVARD UNIV,SCH MED,CUTANEOUS BIOL RES CTR,CHARLESTOWN,MA 02129, USA. RI Fekete, Donna/B-9170-2009 OI Fekete, Donna/0000-0003-0662-0246 NR 25 TC 258 Z9 262 U1 0 U2 4 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 SN 0091-679X J9 METHOD CELL BIOL PY 1996 VL 51 BP 185 EP 218 DI 10.1016/S0091-679X(08)60629-9 PG 34 WC Cell Biology SC Cell Biology GA BF52V UT WOS:A1996BF52V00010 PM 8722477 ER PT J AU Shen, JA Tate, JE Crum, CP Goodman, ML AF Shen, JA Tate, JE Crum, CP Goodman, ML TI Prevalence of human papillomaviruses (HPV) in benign and malignant tumors of the upper respiratory tract SO MODERN PATHOLOGY LA English DT Article DE human papillomavirus; larynx; nasopharynx; nasal cavity; polymerase chain reaction; restriction fragment length polymorphism; neoplasm ID POLYMERASE CHAIN-REACTION; SQUAMOUS-CELL CARCINOMAS; EPSTEIN-BARR-VIRUS; LARYNGEAL CARCINOMA; INSITU HYBRIDIZATION; NASOPHARYNGEAL CARCINOMA; SINONASAL PAPILLOMAS; IMMATURE METAPLASIA; DNA HYBRIDIZATION; CONSENSUS PRIMERS AB The role for human papillomavirus (HPV) in head and neck tumors is not established. To evaluate the possible role of HPV in head and neck neoplasms, 22 cases of laryngeal squamous papilloma (LP), 32 cases of laryngeal squamous cell carcinoma (LSCC), 40 cases of nasal inverted papilloma (NIP), 14 cases of nasal squamous cell carcinoma (NSCC), and 40 cases of nasopharyngeal carcinoma (NPC) were evaluated for the presence of HPV DNA using the polymerase chain reaction (PCR) with two sets of primers in separate reactions: HPV-L1 consensus primers, HPV16 and HPV18 E7 primers for HPV nucleic acid detection. Restriction fragment length polymorphism of L1 PCR product was used for typing of HPV. Overall, HPV DNA was detected in 18 of 22 cases (81.8%) of LP, 3 of 32 cases (9.4%) of LSCC, 17 of 40 cases (42.5%) of NIP, 3 of 14 cases of NSCC (21.4%), and none of 40 cases of NPC. HPV6 was found more frequently in LP and HPV11 in NIP (P < 0.001). In the three HPV positive LSCCs, two cases had previous LP and were HPV6 and HPV11 positive, as were the prior papillomas. One other case was HPV18 positive. Only HPV16 was found in the NSCC patients. There was no significant difference in the index of HPV positivity between the NSCC cases associated with (16.7%) and without MP (25.6%). Our results suggest that HPV plays a role in the development of both LP and NIP, and that similar viral types (i.e., HPV6 and HPV11) may exhibit relative differences in their tissue specificity. HPV appears to be of limited importance as a co-factor in LSCC and NPC lesions, indicating differences in the pathogenesis between papillomas and carcinomas in the upper respiratory tract. C1 MASSACHUSETTS EYE & EAR INFIRM,DEPT OTOLARYNGOL,BOSTON,MA 02114. MASSACHUSETTS GEN HOSP,DEPT PATHOL,BOSTON,MA 02114. BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,BOSTON,MA. NR 45 TC 42 Z9 44 U1 1 U2 2 PU WILLIAMS & WILKINS PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 SN 0893-3952 J9 MODERN PATHOL JI Mod. Pathol. PD JAN PY 1996 VL 9 IS 1 BP 15 EP 20 PG 6 WC Pathology SC Pathology GA TT421 UT WOS:A1996TT42100003 PM 8821950 ER PT J AU Jeggo, PA Jackson, SP Taccioli, GE AF Jeggo, PA Jackson, SP Taccioli, GE TI Identification of the catalytic subunit of DNA dependent protein kinase as the product of the mouse scid gene SO MOLECULAR ANALYSIS OF DNA REARRANGEMENTS IN THE IMMUNE SYSTEM SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Review ID STRAND-BREAK-REPAIR; COMBINED IMMUNE-DEFICIENCY; RAY SENSITIVE MUTANTS; HAMSTER OVARY CELLS; SITE-SPECIFIC RECOMBINATION; V(D)J RECOMBINATION; IONIZING-RADIATION; END-BINDING; JUNCTIONAL SEQUENCES; HUMAN CHROMOSOME-8 C1 UNIV CAMBRIDGE,WELLCOME CRC INST,CAMBRIDGE CB2 1QR,ENGLAND. UNIV CAMBRIDGE,DEPT ZOOL,CAMBRIDGE CB2 1QR,ENGLAND. CHILDRENS HOSP,HOWARD HUGHES MED INST,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115. HARVARD UNIV,SCH MED,CTR BLOOD RES,BOSTON,MA 02115. RP Jeggo, PA (reprint author), UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,E SUSSEX,ENGLAND. RI Dry, Kate/I-2328-2014 FU Wellcome Trust NR 53 TC 10 Z9 11 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 217 BP 79 EP 89 PG 11 WC Immunology; Microbiology SC Immunology; Microbiology GA BJ66K UT WOS:A1996BJ66K00006 PM 8787619 ER PT J AU Li, ZY Alt, FW AF Li, ZY Alt, FW TI Identification of the XRCC4 gene: Complementation of the DSBR and V(D)J recombination defects of XR-1 cells SO MOLECULAR ANALYSIS OF DNA REARRANGEMENTS IN THE IMMUNE SYSTEM SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Review ID STRAND BREAK-REPAIR; PRE-B CELLS; DNA-REPAIR; MUTANTS; SENSITIVITY; LINES; MICE C1 HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115. RP Li, ZY (reprint author), HARVARD UNIV,SCH MED,CTR BLOOD RES,200 LONGWOOD AVE,BOSTON,MA 02115, USA. FU NIAID NIH HHS [AI35714, AI20047] NR 27 TC 4 Z9 4 U1 0 U2 0 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 217 BP 143 EP 150 PG 8 WC Immunology; Microbiology SC Immunology; Microbiology GA BJ66K UT WOS:A1996BJ66K00010 PM 8787623 ER PT J AU Nan, XS Tate, P Li, E Bird, A AF Nan, XS Tate, P Li, E Bird, A TI DNA methylation specifies chromosomal localization of MeCP2 SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID EMBRYONIC STEM-CELLS; CPG BINDING-PROTEIN; DROSOPHILA-MELANOGASTER; TARGETING SEQUENCE; GENE; HETEROCHROMATIN; IDENTIFICATION; METHYLTRANSFERASE; MUTATION AB MeCP2 is a chromosomal protein that is concentrated in the centromeric heterochromatin of mouse cells. In vitro, the protein binds preferentially to DNA containing a single symmetrically methylated CpG. To find out whether the heterochromatic localization of MeCP2 depended on DNA methylation, we transiently expressed MeCP2-LacZ fusion proteins in cultured cells. Intact protein was targeted to heterochromatin in wild-type cells but was inefficiently localized in mutant cells with low levels of genomic DNA methylation, Deletions within MeCP2 showed that localization to heterochromatin required the 85-amino-acid methyl-CpG binding domain but not the remainder of the protein. Thus MeCP2 is a methyl-CpG-binding protein in vivo and is likely to be a major mediator of downstream consequences of DNA methylation. C1 UNIV EDINBURGH,INST CELL & MOLEC BIOL,EDINBURGH EH9 3JR,MIDLOTHIAN,SCOTLAND. MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,BOSTON,MA 02129. NR 36 TC 234 Z9 239 U1 1 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1325 MASSACHUSETTS AVENUE, NW, WASHINGTON, DC 20005-4171 SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD JAN PY 1996 VL 16 IS 1 BP 414 EP 421 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA TL345 UT WOS:A1996TL34500046 PM 8524323 ER PT J AU Missero, C Dotto, GP AF Missero, C Dotto, GP TI p21(WAF1/CIP1) and terminal differentiation control of normal epithelia SO MOLECULAR AND CELLULAR DIFFERENTIATION LA English DT Article DE keratinocytes; cell cycle; p105-Rb; p300 ID MOUSE EPIDERMAL-CELLS; KERATINOCYTE DIFFERENTIATION; CELLULAR-DIFFERENTIATION; E1A ONCOPROTEIN; EXPRESSION; GROWTH; PHOSPHORYLATION; TRANSCRIPTION; CYCLE AB Induction of the cell cycle inhibitor p21(WAF1/CIP1) occurs as an early and specific event in most, if not all, terminally differentiating cells tested so far. In the present review, we will focus on the role and control of p21(WAF1/CIP1) function in terminal differentiation of normal epithelial cells, and keratinocytes in particular. As in other cells, increased p21 expression can explain, at least in part, the observed inhibition of CDK activity in terminally differentiating keratinocytes. However, p21 function in differentiation is not indispensable, and may be compensated by a number of additional cell cycle regulatory events, some of which are common to most cells, and others which are cell type specific. One of the main consequences of CDK inhibition is an increase in the growth suppressing activity of p105-Rb and related proteins. However, these proteins may not be the major determinants of growth arrest in terminally differentiating epithelial cells, and a more important role may be played by other, as yet unidentified CDK substrates. The specific modifications of the transcriptional apparatus which are associated with cell cycle arrest in terminal differentiation are poorly understood. Studies of the p21 promoter already indicate that induction of its activity is controlled by transcription factors of the myoD family in myoblasts but not in keratinocytes, while in these latter cells the function of a novel transcriptional coactivator, p300, is required. Future studies of epithelial as well as other terminally differentiating cells will likely focus on the complex interplay between specific differentiation signals and the modifications of the transcriptional and cell cycle apparatus which lead to growth arrest. C1 MASSACHUSETTS GEN HOSP,CUTANEOUS BIOL RES CTR,CHARLESTOWN,MA 02129. HARVARD UNIV,SCH MED,CHARLESTOWN,MA 02129. NR 69 TC 11 Z9 11 U1 0 U2 0 PU CRC PRESS INC PI BOCA RATON PA 2000 CORPORATE BLVD NW, BOCA RATON, FL 33431 SN 1065-3074 J9 MOL CELL DIFFER JI Mol. Cell. Differ. PY 1996 VL 4 IS 1 BP 1 EP 16 PG 16 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA UP326 UT WOS:A1996UP32600002 ER PT S AU Kronenberg, HM Karaplis, AC Lanske, B AF Kronenberg, HM Karaplis, AC Lanske, B BE deCrombrugghe, B Horton, WA Olsen, BR Ramirez, F TI Role of parathyroid hormone-related protein in skeletal development SO MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular and Developmental Biology of Cartilage CY SEP 27-30, 1995 CL BETHESDA, MD SP New York Acad Sci ID PEPTIDE C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. MCGILL UNIV,MONTREAL,PQ H3T 1E2,CANADA. RP Kronenberg, HM (reprint author), MASSACHUSETTS GEN HOSP,ENDOCRINE UNIT,BOSTON,MA 02114, USA. NR 5 TC 10 Z9 12 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-010-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 785 BP 119 EP 123 PG 5 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BF92H UT WOS:A1996BF92H00013 PM 8702116 ER PT S AU Chen, Q Johnson, DM Haudenschild, DR Goetinck, PF AF Chen, Q Johnson, DM Haudenschild, DR Goetinck, PF BE deCrombrugghe, B Horton, WA Olsen, BR Ramirez, F TI Cartilage matrix protein: Expression patterns in chicken, mouse, and human SO MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular and Developmental Biology of Cartilage CY SEP 27-30, 1995 CL BETHESDA, MD SP New York Acad Sci ID COLLAGEN C1 HARVARD UNIV,SCH MED,CHARLESTOWN,MA 02129. RP Chen, Q (reprint author), MASSACHUSETTS GEN HOSP,CUTANEOUS BIOL RES CTR,BLDG 149,13TH ST,CHARLESTOWN,MA 02129, USA. RI Haudenschild, Dominik/B-2381-2008; Chen, Qian/C-4354-2011 OI Haudenschild, Dominik/0000-0001-9947-9864; Chen, Qian/0000-0003-4406-5618 FU NICHD NIH HHS [HD22016]; NIGMS NIH HHS [P20 GM104937] NR 6 TC 6 Z9 6 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-010-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 785 BP 238 EP 240 PG 3 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BF92H UT WOS:A1996BF92H00035 PM 8702140 ER PT S AU Hofer, U Kahoussi, B Trelstad, J Zavatarelli, M Goetinck, PF AF Hofer, U Kahoussi, B Trelstad, J Zavatarelli, M Goetinck, PF BE deCrombrugghe, B Horton, WA Olsen, BR Ramirez, F TI Expression of functional link protein domains using an avian-specific retroviral vector SO MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular and Developmental Biology of Cartilage CY SEP 27-30, 1995 CL BETHESDA, MD SP New York Acad Sci ID HYALURONATE; GENE C1 HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,CUTANEOUS BIOL RES CTR,CHARLESTOWN,MA 02129. FU NICHD NIH HHS [HD 22016] NR 7 TC 0 Z9 0 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-010-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 785 BP 271 EP 273 PG 3 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BF92H UT WOS:A1996BF92H00046 PM 8702152 ER PT S AU Raftery, LA Wisotzkey, RG AF Raftery, LA Wisotzkey, RG BE deCrombrugghe, B Horton, WA Olsen, BR Ramirez, F TI Characterization of Medea, a gene required for maximal function of the Drosophila BMP homolog Decapentaplegic SO MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular and Developmental Biology of Cartilage CY SEP 27-30, 1995 CL BETHESDA, MD SP New York Acad Sci ID RECEPTOR; DPP; SAXOPHONE; ELEMENTS; PATTERN C1 HARVARD UNIV,SCH MED,CHARLESTOWN,MA 02129. RP Raftery, LA (reprint author), MASSACHUSETTS GEN HOSP,CUTANEOUS BIOL RES CTR,CHARLESTOWN,MA 02129, USA. RI Raftery, Laurel/H-1406-2012 OI Raftery, Laurel/0000-0003-4797-4163 NR 10 TC 8 Z9 8 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-010-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 785 BP 318 EP 320 PG 3 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BF92H UT WOS:A1996BF92H00061 PM 8702167 ER PT S AU Yang, BH Yang, BL Goetinck, PF AF Yang, BH Yang, BL Goetinck, PF BE deCrombrugghe, B Horton, WA Olsen, BR Ramirez, F TI Construction and expression of a functional recombinant gene for proteoglycan SO MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular and Developmental Biology of Cartilage CY SEP 27-30, 1995 CL BETHESDA, MD SP New York Acad Sci ID CORE PROTEIN; CHONDROCYTES; SYNTHESIZE; NANOMELIA C1 MASSACHUSETTS GEN HOSP,CUTANEOUS BIOL RES CTR,CHARLESTOWN,MA 02129. HARVARD UNIV,SCH MED,CHARLESTOWN,MA 02129. FU NICHD NIH HHS [HD 22016] NR 5 TC 1 Z9 1 U1 0 U2 1 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-010-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 785 BP 356 EP 359 PG 4 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BF92H UT WOS:A1996BF92H00073 PM 8702180 ER PT S AU Yelick, PC Driever, W Neuhauss, S Stashenko, P AF Yelick, PC Driever, W Neuhauss, S Stashenko, P BE deCrombrugghe, B Horton, WA Olsen, BR Ramirez, F TI Craniofacial cartilage development in zebrafish SO MOLECULAR AND DEVELOPMENTAL BIOLOGY OF CARTILAGE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT Conference on Molecular and Developmental Biology of Cartilage CY SEP 27-30, 1995 CL BETHESDA, MD SP New York Acad Sci C1 MASSACHUSETTS GEN HOSP E,CARDIOVASC RES CTR,CHARLESTOWN,MA 02129. RP Yelick, PC (reprint author), FORSYTH DENT CTR,140 FENWAY,BOSTON,MA 02115, USA. NR 6 TC 6 Z9 6 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 1-57331-010-7 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 785 BP 360 EP 361 PG 2 WC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Multidisciplinary Sciences SC Biochemistry & Molecular Biology; Cell Biology; Developmental Biology; Science & Technology - Other Topics GA BF92H UT WOS:A1996BF92H00074 PM 8702181 ER PT J AU Cohen, EA Subbramanian, RA Gottlinger, HG AF Cohen, EA Subbramanian, RA Gottlinger, HG TI Role of auxiliary proteins in retroviral morphogenesis SO MORPHOGENESIS AND MATURATION OF RETROVIRUSES SE CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY LA English DT Review ID HUMAN-IMMUNODEFICIENCY-VIRUS; TYPE-1 VPU PROTEIN; CHIMERIC ENVELOPE GLYCOPROTEINS; MURINE LEUKEMIA VIRUSES; SOR GENE-PRODUCT; CYTOPLASMIC DOMAIN; VIF PROTEIN; ENDOPLASMIC-RETICULUM; NUCLEAR-LOCALIZATION; VIRION INCORPORATION C1 HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV HUMAN RETROVIROL,BOSTON,MA 02115. HARVARD UNIV,SCH MED,DEPT PATHOL,BOSTON,MA 02115. RP Cohen, EA (reprint author), UNIV MONTREAL,DEPT MICROBIOL & IMMUNOL,LAB RETROVIROL HUMAINE,CP 6128,SUCCURSALE CTR VILLE,MONTREAL,PQ H3C 3J7,CANADA. FU NIAID NIH HHS [AI34267, AI29873] NR 102 TC 31 Z9 31 U1 1 U2 2 PU SPRINGER-VERLAG BERLIN PI BERLIN 33 PA HEIDELBERGER PLATZ 3, W-1000 BERLIN 33, GERMANY SN 0070-217X J9 CURR TOP MICROBIOL JI Curr.Top.Microbiol.Immunol. PY 1996 VL 214 BP 219 EP 235 PG 17 WC Immunology; Microbiology SC Immunology; Microbiology GA BJ59F UT WOS:A1996BJ59F00007 PM 8791729 ER PT J AU Melder, RJ Koenig, GC Munn, LL Jain, RK AF Melder, RJ Koenig, GC Munn, LL Jain, RK TI Adhesion of activated natural killer cells to tumor necrosis factor-alpha-treated endothelium under physiological flow conditions SO NATURAL IMMUNITY LA English DT Article DE natural killer cell; adhesion; flow; endothelium; tumor necrosis factor-alpha ID UNDERLYING LYMPHOCYTE RECIRCULATION; POSITRON EMISSION TOMOGRAPHY; NEUTROPHIL ADHESION; LEUKOCYTE ADHESION; L-SELECTIN; ADOPTIVE IMMUNOTHERAPY; VASCULAR ENDOTHELIUM; INVIVO; KINETICS; VENULES AB Adhesion of activated natural killer (A-NK) cells to activated and nonactivated endothelial cells in vitro was studied under dynamic flow conditions. Endothelial cells grown on glass slides were either treated with tumor necrosis factor-alpha (TNF alpha) or medium, then placed into a flow chamber over which suspensions of A-NK cells were passed using a range of defined shear stress levels. Significant numbers of binding cells could be consistently observed at shear stress levels less than 3 dyn/cm(2) on TNF alpha-activated endothelium or at 0.59 dyn/cm(2) on nonactivated endothelium. Stable adhesion occurred rapidly following the initial interaction of the flowing cells with the endothelium in the absence of detectable rolling. Pretreatment of the A-NK cells with monoclonal antibodies directed against CD18 (LFA-1) or CD49d (VLA-4) resulted in a significant reduction in the number of binding cells. Simultaneous treatment with both monoclonal antibodies eliminated all A-NK adhesion occurring over 0.5 dyn/cm(2). Pretreatment of the endothelial cells with antibodies against E- or P-selectin resulted in a small but significant reduction in binding only at 0.5 dyn/cm(2). The binding efficiency of the A-NK cells was similar to that previously observed for T lymphocytes under the same conditions. Once bound, approximately half of the adherent cells could resist detachment when exposed to wall shear stresses over 12 dyn/cm(2). These findings indicate that A-NK cell adhesion to activated endothelium can occur under shear stress conditions which are representative of postcapillary venules and that this binding is mediated principally by both CD18 and CD49d. A-NK cell adhesion also occurs to nonactivated endothelium but only at wall shear stress levels less than 1 dyn/cm(2). C1 HARVARD UNIV,SCH MED,BOSTON,MA 02114. RP Melder, RJ (reprint author), MASSACHUSETTS GEN HOSP,DEPT RADIAT ONCOL,EDWIN L STEELE LAB,BOSTON,MA 02114, USA. RI Munn, Lance/L-3950-2016 OI Munn, Lance/0000-0003-0698-7232 NR 51 TC 11 Z9 11 U1 0 U2 0 PU KARGER PI BASEL PA ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND SN 1018-8916 J9 NAT IMMUN JI Nat. Immun. PY 1996 VL 15 IS 2-3 BP 154 EP 163 PG 10 WC Cell Biology; Immunology SC Cell Biology; Immunology GA WL401 UT WOS:A1996WL40100009 PM 9162265 ER PT J AU Galli, J Li, LS Glaser, A Ostenson, CG Jiao, H FakhraiRad, H Jacob, HJ Lander, ES Luthman, H AF Galli, J Li, LS Glaser, A Ostenson, CG Jiao, H FakhraiRad, H Jacob, HJ Lander, ES Luthman, H TI Genetic analysis of non-insulin dependent diabetes mellitus in the GK rat SO NATURE GENETICS LA English DT Article ID IMPAIRED GLUCOSE-TOLERANCE; EXPRESSION; HISTORY; REGION; CELLS AB Non-insulin dependent diabetes mellitus (NIDDM) is a major public health problem, but its aetiology remains poorly understood. We have performed a comprehensive study of the genetic basis of diabetes in the Goto-Kakizaki (GK) rat, the most widely used animal model of non-obese NIDDM. The genetic dissection of NIDDM using this model has allowed us to map three independent loci involved in the disease. In addition, we identify a major factor affecting body weight, but not glucose tolerance, on chromosome 7 and map a further 10 regions that are suggestive for linkage. We conclude that NIDDM is polygenic and fasting hyperglycaemia and postprandial hyperglycaemia clearly have distinct genetic bases. C1 KAROLINSKA HOSP,DEPT MOLEC MED,ROLF LUFT CTR DIABET RES,S-17176 STOCKHOLM,SWEDEN. MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,BOSTON,MA 02129. WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142. MIT,DEPT BIOL,CAMBRIDGE,MA 02139. NR 34 TC 219 Z9 219 U1 0 U2 8 PU NATURE PUBLISHING CO PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1996 VL 12 IS 1 BP 31 EP 37 DI 10.1038/ng0196-31 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA TM555 UT WOS:A1996TM55500011 PM 8528247 ER PT J AU Brown, DM Provoost, AP Daly, MJ Lander, ES Jacob, HJ AF Brown, DM Provoost, AP Daly, MJ Lander, ES Jacob, HJ TI Renal disease susceptibility and hypertension are under independent genetic control in the fawn-hooded rat SO NATURE GENETICS LA English DT Article ID WISTAR-KYOTO RATS; BLOOD-PRESSURE; GLOMERULAR SCLEROSIS; FAMILIAL RISK; LINKAGE; LOCI; COSEGREGATION; PATHOGENESIS; CHROMOSOME-1; ABLATION AB Hypertension, diabetes and hyperlipidemia are risk factors for life-threatening complications such as end-stage renal disease, coronary artery disease and stroke. Why some patients develop complications is unclear, but only susceptibility genes may be involved. To test this notion, we studied crosses involving the fawn-hooded rat, an animal model of hypertension that develops chronic renal failure. Here, we report the localization of two genes, Rf-1 and Rf-2, responsible for about half of the genetic variation in key indices of renal impairment. In addition, we localize a gene, Bpfh-1, responsible for about 26% of the genetic variation in blood pressure. Rf-1 strongly affects the risk of renal impairment, but has no significant effect on blood pressure. Our results show that susceptibility to a complication of hypertension is under at feast partially independent genetic control from susceptibility to hypertension itself. C1 HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,CARDIOVASC RES CTR,BOSTON,MA 02129. ERASMUS UNIV ROTTERDAM,DEPT PEDIAT SURG,ROTTERDAM,NETHERLANDS. WHITEHEAD INST BIOMED RES,MIT,CTR GENOME RES,CAMBRIDGE,MA 02142. MIT,DEPT BIOL,CAMBRIDGE,MA 02139. NR 56 TC 227 Z9 226 U1 0 U2 3 PU NATURE PUBLISHING CO PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 SN 1061-4036 J9 NAT GENET JI Nature Genet. PD JAN PY 1996 VL 12 IS 1 BP 44 EP 51 DI 10.1038/ng0196-44 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA TM555 UT WOS:A1996TM55500013 PM 8528250 ER PT J AU Tanzi, RE AF Tanzi, RE TI Neuropathology in the Down's syndrome brain SO NATURE MEDICINE LA English DT Editorial Material ID ALZHEIMERS-DISEASE; PROTEIN RP Tanzi, RE (reprint author), MASSACHUSETTS GEN HOSP,GENET & AGING UNIT,BOSTON,MA 02129, USA. FU Telethon [C.23 BIS] NR 11 TC 14 Z9 15 U1 1 U2 1 PU NATURE PUBLISHING CO PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 SN 1078-8956 J9 NAT MED JI Nat. Med. PD JAN PY 1996 VL 2 IS 1 BP 31 EP 32 DI 10.1038/nm0196-31 PG 2 WC Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Cell Biology; Research & Experimental Medicine GA TP579 UT WOS:A1996TP57900032 PM 8564833 ER PT J AU Freedman, AR Zhu, HH Levine, JD Kalams, S Scadden, DT AF Freedman, AR Zhu, HH Levine, JD Kalams, S Scadden, DT TI Generation of human T lymphocytes from bone marrow CD34(+) cells in vitro SO NATURE MEDICINE LA English DT Article ID HEMATOPOIETIC STEM-CELLS; HUMAN FETAL THYMOCYTES; EARLY EVENTS; HUMAN THYMUS; PRECURSORS; INFECTION; INVITRO; DIFFERENTIATION; DEPLETION; INVIVO AB Analysis of the events that regulate development of red blood cells or granulocytes has led to therapies altering clinical conditions associated with anemia or neutropenia. The development of therapeutic approaches to target conditions associated with lymphopenia, such as AIDS, has been thwarted by limited techniques for studying T-lymphocyte development. We describe an in vitro system in which human bone marrow CD34 cells proliferate, acquire the expression of the lymphoid-specific RAG-2 gene and a broad repertoire of rearranged T-cell receptor genes, develop the ability to produce T cell-specific interleukin-2 and achieve a range of T-cell immunophenotypes. The cells also become susceptible to infection with the T-lymphotropic strain of human immunodeficiency virus-1, HIV-1(IIB). This culture system induces human T lymphopoiesis and may permit further analysis of the events regulating human T-lineage differentiation. It provides a preclinical model for screening stem cell gene therapies directed toward AIDS. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,AIDS RES CTR,INFECT DIS UNIT,BOSTON,MA 02129. HARVARD UNIV,NEW ENGLAND DEACONESS HOSP,SCH MED,DIV HEMATOL ONCOL,BOSTON,MA 02129. FU NHLBI NIH HHS [R01 HL 44851] NR 30 TC 56 Z9 56 U1 0 U2 0 PU NATURE PUBLISHING CO PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 SN 1078-8956 J9 NAT MED JI Nat. Med. PD JAN PY 1996 VL 2 IS 1 BP 46 EP 51 DI 10.1038/nm0196-46 PG 6 WC Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Cell Biology; Research & Experimental Medicine GA TP579 UT WOS:A1996TP57900036 PM 8564838 ER PT J AU Sherrard, DJ Hercz, G Pei, Y Segre, G AF Sherrard, DJ Hercz, G Pei, Y Segre, G TI The aplastic form of renal osteodystrophy SO NEPHROLOGY DIALYSIS TRANSPLANTATION LA English DT Article; Proceedings Paper CT ISN Satellite Seville Symposium on Renal Bone Disease, Parathyroid Hormone and Vitamin-D CY JUL 07-11, 1995 CL SEVILLE, SPAIN DE bone; calcium; parathyroid hormone; aplastic ID BONE-DISEASE; ALUMINUM; FAILURE AB That bone disease accompanies renal failure has been known for over 100 years. This bone disease (renal osteodystrophy) has been variously attributed to hyperparathyroidism, vitamin D deficiency, aluminium toxicity, iron toxicity, uraemia, and a host of other aetiologies. In addition, the form the bone disease takes has been variously described as osteitis fibrosa, osteomalacia, mixed uraemic osteodystrophy and the aplastic (adynamic) lesion. In this manuscript we will focus on the aetiology, consequences, diagnosis and possible management of the aplastic form of the disease. The renal osteodystrophy study was a prospective, cross-sectional study of renal bone disease in a largely unselected population of patients receiving dialysis in three hospitals in Toronto. A variety of non-invasive data (parathyroid hormone (PTH), aluminium, etc.) and bone histology were obtained and analysed to assess pathogenesis, diagnostic criteria and management. We have defined the aplastic lesion as having low bone formation without a marked increase in unmineralized osteoid (i.e. excluding osteomalacia). We have noted that it may be associated with increased aluminium or little to no aluminium. With increased aluminium the patients have a poorer prognosis both with regards to bone disease and mortality, and they should be managed appropriately to alleviate aluminium toxicity. With lesser amounts of aluminium, morbidity and mortality are less severely impacted, but not normal. We have shown that the low bone formation, of the aplastic lesion without aluminium may be 'normalized' by increasing PTH levels. It is concluded that aplastic bone disease carries adverse consequences both in terms of bone problems and survival. In the absence of aluminium toxicity the stimulation of PTH effectively corrects the bone formation abnormality. Whether this will alleviate the adverse consequences will be difficult to study. Avoiding the problem by not over-suppressing PTH seems a reasonable approach at this point. C1 SEATTLE VET ADM HOSP,DEPT MED,SEATTLE,WA. UNIV WASHINGTON,DEPT MED,SEATTLE,WA 98195. MASSACHUSETTS GEN HOSP,ENDOCRINE UNIT,BOSTON,MA 02114. UNIV TORONTO,TORONTO,ON,CANADA. NR 12 TC 28 Z9 28 U1 2 U2 2 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0931-0509 J9 NEPHROL DIAL TRANSPL JI Nephrol. Dial. Transplant. PY 1996 VL 11 SU 3 BP 29 EP 31 PG 3 WC Transplantation; Urology & Nephrology SC Transplantation; Urology & Nephrology GA UZ945 UT WOS:A1996UZ94500007 PM 8840308 ER PT J AU Coburn, JW Tan, AU Levine, BS Mazess, RB Kyllo, DM Knutson, JC Bishop, CW AF Coburn, JW Tan, AU Levine, BS Mazess, RB Kyllo, DM Knutson, JC Bishop, CW TI 1 alpha-hydroxy-vitamin D2: A new look at an 'old' compound SO NEPHROLOGY DIALYSIS TRANSPLANTATION LA English DT Article; Proceedings Paper CT ISN Satellite Seville Symposium on Renal Bone Disease, Parathyroid Hormone and Vitamin-D CY JUL 07-11, 1995 CL SEVILLE, SPAIN DE 1 alpha-hydroxy-vitamin D-2; haemodialysis; hyperparathyroidism; renal osteodystrophy; vitamin D ID CALCIUM-METABOLISM; VITAMIN-D; POSTMENOPAUSAL OSTEOPOROSIS; RATS; 1-ALPHA-HYDROXYVITAMIN-D2; 1-ALPHA-HYDROXYCHOLECALCIFEROL; HYPERPARATHYROIDISM AB Calcitriol is effective in suppressing PTH levels in haemodialysis patients with hyperparathyroidism but has a low therapeutic index. There is a search for other vitamin D sterols that suppress PTH but cause less hypercalcaemia. We review evidence that 1 alpha-hydroxy-vitamin D-2 (1 alpha-D-2) may be an effective and safer alternative to calcitriol. In vitamin D-deficient rats, 1 alpha-D-2 is equipotent to 1 alpha-D-3, which is converted to calcitriol before it acts; but, in normal rats, 1 alpha-D-2 is much less toxic at high doses. In osteopenia models, either steroid-induced or following ovariectomy, 1 alpha-D-2 is equal to or more effective than 1 alpha-D-3 in preventing bone loss but causes less hypercalciuria. Studies in osteoporotic women reveal minimal hypercalciuria with 1 alpha-D-2 at doses up to 4 mu g/day, data suggesting greater safety than reported with calcitriol or 1 alpha-D-3. Preliminary data in haemodialysis patients with secondary hyperparathyroidism demonstrate the efficacy of 1 alpha-D-2 in suppressing PTH levels with minimal untoward effects on serum Ca and no effects on serum P. Taken together, these observations suggest that 1 alpha-D-2 deserves strong consideration as a therapeutic agent for secondary hyperparathyroidism associated with end-stage renal disease. C1 W LOS ANGELES VET AFFAIRS MED CTR,NEPHROL SECT,RES SERV,WADSWORTH DIV,LOS ANGELES,CA 90073. LUNAR CORP INC,MADISON,WI. UNIV CALIF LOS ANGELES,SCH MED,DEPT MED,LOS ANGELES,CA. RP Coburn, JW (reprint author), W LOS ANGELES VET AFFAIRS MED CTR,NEPHROL SECT,MED SERV,WADSWORTH DIV,11301 WILSHIRE BLVD,LOS ANGELES,CA 90073, USA. NR 24 TC 25 Z9 25 U1 0 U2 1 PU OXFORD UNIV PRESS UNITED KINGDOM PI OXFORD PA WALTON ST JOURNALS DEPT, OXFORD, ENGLAND OX2 6DP SN 0931-0509 J9 NEPHROL DIAL TRANSPL JI Nephrol. Dial. Transplant. PY 1996 VL 11 SU 3 BP 153 EP 157 PG 5 WC Transplantation; Urology & Nephrology SC Transplantation; Urology & Nephrology GA UZ945 UT WOS:A1996UZ94500031 PM 8840332 ER PT S AU Gonzalez, RG AF Gonzalez, RG BE Wurtman, RJ Corkin, S Growdon, JH Nitsch, RM TI Molecular and functional magnetic resonance neuroimaging for the study of dementia SO NEUROBIOLOGY OF ALZHEIMER'S DISEASE SE ANNALS OF THE NEW YORK ACADEMY OF SCIENCES LA English DT Article; Proceedings Paper CT 8th Meeting of the International-Study-Group-on-the-Pharmacology-of-Memory-Disorders-Associ ated-with-Aging on the Neurobiology of Alzheimers Disease CY FEB 17-19, 1995 CL ZURICH, SWITZERLAND SP Int Study Grp Pharm Memory Disorders Associated Aging, Ctr Brain Sci & Metabolism Charitable Trust, Cambridge Massachusetts, Alzheimers Assoc, French Fdn, Helen Bader Fdn, John D & Catherine T MacArthur Fdn ID N-ACETYL-ASPARTATE; ALZHEIMERS-DISEASE; NEUROFIBRILLARY TANGLES; ENERGY-METABOLISM; SENILE PLAQUES; BRAIN; SPECTROSCOPY; TOMOGRAPHY; LOCALIZATION; INVIVO AB The living brain's structure, function, and underlying chemistry are increasingly being revealed in sharpened detail by the extraordinary evolution of magnetic resonance (MR) technology. Recent years have ushered in a wealth of new information about neurophysiology and pathological states owing to such technologies as functional MR imaging (fMRI), MR spectroscopy (MRS), and MR spectroscopic imaging (MRSI). These advances are of substantial benefit in the study of the dementias, especially Alzheimer's disease (AD). One primary objective of our laboratory at the Massachusetts General Hospital NMR Center is to utilize these extant and emerging MR technologies to further understanding of the human brain as it undergoes assault by AD. Our approach is guided by the belief that the pathological states observed in the AD brain must be accompanied by structural, chemical and/or physiological changes that can be made visible in an in vivo MR study. Quantitative measurements by MRI medical temporal lobe structures have been shown to be abnormal by several groups including our own. This use of MRI will be reviewed by others. In this paper, I will review recent advances in the application of MR for the study of chemical and functional brain abnormalities in dementia, and in particular to the investigation of AD. C1 HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,NMR CTR,BOSTON,MA 02129. HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,NEURORADIOL SECT,BOSTON,MA 02129. NR 29 TC 5 Z9 5 U1 0 U2 2 PU NEW YORK ACAD SCIENCES PI NEW YORK PA 2 EAST 63RD ST, NEW YORK, NY 10021 SN 0077-8923 BN 0-89766-974-6 J9 ANN NY ACAD SCI JI Ann.NY Acad.Sci. PY 1996 VL 777 BP 37 EP 48 DI 10.1111/j.1749-6632.1996.tb34399.x PG 12 WC Multidisciplinary Sciences; Clinical Neurology; Neurosciences SC Science & Technology - Other Topics; Neurosciences & Neurology GA BF30Z UT WOS:A1996BF30Z00005 ER EF