Package: karlen
Title: Real-Time PCR Data Sets by Karlen et al. (2007)
Version: 0.0.2
Authors@R: c(
    person("Ramiro", "Magno", , "rmagno@pattern.institute", role = c("aut", "cre"),
           comment = c(ORCID = "0000-0001-5226-3441")),
    person("Pattern Institute", role = c("cph", "fnd"))
  )
Description: Real-time quantitative polymerase chain reaction (qPCR) data
    sets by Karlen et al. (2007) <doi:10.1186/1471-2105-8-131>. Provides one
    single tabular tidy data set in long format, encompassing 32
    dilution series, for seven PCR targets and four biological samples. The
    targeted amplicons are within the murine genes: Cav1, Ccn2, Eln, Fn1, Rpl27,
    Hspg2, and Serpine1, respectively. Dilution series: scheme 1 (Cav1, Eln,
    Hspg2, Serpine1): 1-fold, 10-fold, 50-fold, and 100-fold; scheme 2 (Ccn2,
    Rpl27, Fn1): 1-fold, 10-fold, 50-fold, 100-fold and 1000-fold.
    For each concentration there are five replicates, except for the 1000-fold
    concentration, where only two replicates were performed. Each amplification
    curve is 40 cycles long. Original raw data file is Additional file 2 from
    "Statistical significance of quantitative PCR" by Y. Karlen, A. McNair,
    S. Perseguers, C. Mazza, and N. Mermod (2007)
    <https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-8-131/MediaObjects/12859_2006_1503_MOESM2_ESM.ZIP>.
License: CC BY 4.0
Encoding: UTF-8
RoxygenNote: 7.3.1
Imports: tibble
Depends: R (>= 2.10)
LazyData: true
URL: https://rmagno.eu/karlen/, https://github.com/ramiromagno/karlen
BugReports: https://github.com/ramiromagno/karlen/issues
NeedsCompilation: no
Packaged: 2024-04-28 14:28:17 UTC; rmagno
Author: Ramiro Magno [aut, cre] (<https://orcid.org/0000-0001-5226-3441>),
  Pattern Institute [cph, fnd]
Maintainer: Ramiro Magno <rmagno@pattern.institute>
Repository: CRAN
Date/Publication: 2024-04-30 08:30:07 UTC
Built: R 4.4.3; ; 2025-10-21 13:53:58 UTC; windows
