% Generated by roxygen2: do not edit by hand
% Please edit documentation in R/data.R
\docType{data}
\name{hts_plate_384}
\alias{hts_plate_384}
\title{Example single 384-well screening plate (realistic layout)}
\format{
A data frame with one row per well (all 384) and the following
columns:
\describe{
\item{plate_id}{Plate identifier, \code{"EXP87P01"} (character).}
\item{well}{Well address such as \code{"C03"} (character).}
\item{row}{Plate row letter, \code{"A"}-\code{"P"} (character).}
\item{col}{Plate column number, \code{1}-\code{24} (integer).}
\item{well_type}{Well role: \code{"PC"} (positive control), \code{"NC"} (negative
control), \code{"compound"} or \code{"edgewell"} (empty buffer) (character).}
\item{compound_id}{Compound identifier for test wells, \code{NA} for controls
and edge wells (character).}
\item{concentration}{Compound concentration (micromolar); \code{NA} for
controls and edge wells (numeric).}
\item{signal}{Raw luminescence read-out (numeric).}
}
}
\usage{
hts_plate_384
}
\description{
A single simulated 384-well plate (\code{"EXP87P01"}) whose layout mirrors a real
cell-based high-throughput screen (see \verb{EXP87/}). The two outermost rings of
wells (rows A, B, O, P and columns 1, 2, 23, 24) are kept free of compound to
avoid edge-evaporation artefacts. They still hold medium but no live cells,
so the instrument reads them at the same low, "dead" level as the kill
control; they are stored as \code{"edgewell"} wells (not omitted). The controls
sit inset from the true plate edge in the two innermost flanking columns
(3 and 22), arranged in a rotationally balanced \emph{diagonal} pattern: column 3
holds the positive (kill) control in its top half (rows C-H) and the negative
(DMSO) control in its bottom half (rows I-N), while column 22 mirrors it
(negative top, positive bottom). This diagonal split averages out
spatial/edge signal gradients. Columns 4-21 across the interior rows C-N hold
the test compounds. Used to demonstrate plate-layout heatmaps with control
circling on a 384-well plate.
}
\details{
The data are simulated (see \code{data-raw/make_datasets.R}) and do not
correspond to any real compound. All 384 wells are stored; the empty edge
wells carry only a low background/no-cell reading.
}
\keyword{datasets}
