FN Thomson Reuters Web of Science™
VR 1.0
PT J
AU Bulashev, AK
Borovikov, SN
Serikova, SS
Suranshiev, ZA
Kiyan, VS
Eskendirova, SZ
AF Bulashev, Aitbay K.
Borovikov, Sergey N.
Serikova, Shynar S.
Suranshiev, Zhanbolat A.
Kiyan, Vladimir S.
Eskendirova, Saule Z.
TI Development of an ELISA using anti-idiotypic antibody for diagnosis of
opisthorchiasis
SO FOLIA PARASITOLOGICA
LA English
DT Article
DE Opisthorchis felineus; excretory-secretory antigen; antigenicity;
immunogenicity; monoclonal antibody; polyclonal antibody
ID ENZYME-IMMUNOASSAY; VIVERRINI; INFECTIONS; RESPONSES; PROTEINS;
FELINEUS; ANTIGENS; SYSTEM; CELLS; LIVER
AB Monoclonal antibody specific for an epitope of cretory-secretory antigen protein of Opisthorchis felineus (Rivolta, 1884) (Trematoda: Opisthorchiidae) with a molecular weight of 28 kDa was used in a sandwich enzyme-linked immunosorbent assay (ELISA) for immobilisation of liver fluke specific antigen to the solid phase. Examination of human sera by this ELISA compared with commercial assays demonstrated that the monoclonal antibody epitope is located within this significant parasite protein. Anti-idiotypic antibody specific for the paratope of this monoclonal antibody was obtained by a hybridoma technique. Mimicking an epitope of excretory-secretory antigen of O. felineus, it had the capacity to bind specific antibody and elicit an antibody response. The value of anti-idiotypic antibody as a substitute for the liver fluke antigen was tested by ELISA using serum samples of infected dogs. Anti-idiotypic antibody proved to be of value in both an indirect-ELISA and a competitive-ELISA for diagnosis of opisthorchiasis. Mature trematodes were isolated from all infected animals. The faecal egg counts were negative in dogs with a relatively small number of parasites, despite finding antibodies in serum by ELISA. Substitution of parasite antigen with anti-idiotype avoids the use of experimental animals and also reduces time-consuming steps of antigen preparation.
C1 [Bulashev, Aitbay K.; Borovikov, Sergey N.; Serikova, Shynar S.; Suranshiev, Zhanbolat A.; Kiyan, Vladimir S.] S Seifullin Kazakh Agrotech Univ, Fac Vet & Livestock Technol, Prospect Pobedy 62, Astana 010011, Kazakhstan.
[Eskendirova, Saule Z.] Natl Ctr Biotechnol Republ Kazakhstan, Cell Engn Lab, Astana, Kazakhstan.
RP Bulashev, AK (reprint author), S Seifullin Kazakh Agrotech Univ, Fac Vet & Livestock Technol, Prospect Pobedy 62, Astana 010011, Kazakhstan.
EM aytbay57@mail.ru
FU Ministry of Education and Science of the Republic of Kazakhstan
[0109RK00476, 0112 RK01353]
FX The authors are grateful to S.K. Atygaeva (Astana Infectious Diseases
Hospital, Astana, Kazakhstan) who provided serum samples from patients
suspected of contracting infectious diseases and Orken S. Akibekov,
Lyudmila A. Lider (S. Seifullin Kazakh Agro-Technical University,
Astana, Kazakhstan), Sadibek S. Tokpan (National Veterinary Laboratory,
Astana, Kazakhstan) for their technical support to test fish for the
presence of metacercariae and infect dogs. We are also indebted to Sara
O. Kitaibekova for the revision of the manuscript. This investigation
was financially supported by the Ministry of Education and Science of
the Republic of Kazakhstan within the framework of the projects Nos.
0109RK00476 and 0112 RK01353 for 2009-2011 and 2012-2014, respectively.
NR 36
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U2 8
PU FOLIA PARASITOLOGICA
PI CESKE BUDEJOVICE
PA BRANISOVSKA 31,, CESKE BUDEJOVICE 370 05, CZECH REPUBLIC
SN 0015-5683
EI 1803-6465
J9 FOLIA PARASIT
JI Folia Parasitol.
PD JUL 14
PY 2016
VL 63
AR 025
DI 10.14411/fp.2016.025
PG 8
WC Parasitology
SC Parasitology
GA DV0LJ
UT WOS:000382608700001
ER
PT J
AU Courneya, KS
McNeely, ML
Culos-Reed, SN
Vallance, JK
Bell, GJ
Mackey, JR
Matthews, CE
Morielli, AR
Cook, D
MacLaughlin, S
Farris, MS
Voaklander, S
O'Reilly, R
Friedenreich, CM
AF Courneya, Kerry S.
McNeely, Margaret L.
Culos-Reed, S. Nicole
Vallance, Jeff K.
Bell, Gordon J.
Mackey, John R.
Matthews, Charles E.
Morielli, Andria R.
Cook, Diane
MacLaughlin, Sarah
Farris, Megan S.
Voaklander, Stephanie
O'Reilly, Rachel
Friedenreich, Christine M.
TI The Alberta Moving Beyond Breast Cancer (AMBER) Cohort Study:
Recruitment, Baseline Assessment, and Description of the First 500
Participants
SO BMC CANCER
LA English
DT Article
DE Body composition; Breast cancer; Exercise; Health-related fitness;
Lymphedema; Physical activity; Quality of life; Sedentary behavior;
Survivorship
ID PHYSICAL-ACTIVITY; UNITED-STATES; SURVIVORS; HEALTH; QUESTIONNAIRE;
METAANALYSIS; VALIDITY; LIFE
AB Background: To our knowledge, the Alberta Moving Beyond Breast Cancer (AMBER) Study is the first and only prospective cohort study of breast cancer survivors that includes objectively-measured physical activity (PA), sedentary behavior, health-related fitness (HRF), and biologic mechanisms focused on understanding breast cancer outcomes. The purpose of the present study was to report on the feasibility of recruitment, baseline measurement completion, and the representativeness of the first 500 participants.
Methods: AMBER is enrolling newly diagnosed stage I (>= T1c) to IIIc breast cancer survivors in Alberta, Canada. Baseline assessments are completed soon after diagnosis and include cardiorespiratory fitness, musculoskeletal fitness, body composition, objective and self-reported PA and sedentary behavior, lymphedema, and blood collection.
Results: Between July 2012 and November 2014, AMBER recruited its first 500 participants from a pool of 1,447 (35 %) eligible breast cancer survivors. Baseline HRF assessments were completed on >= 85 % of participants with the exception of upper body strength. Collection of >= 4 days/week of monitoring for the Actigraph GT3X (R) and ActivPAL (R) were obtained from 90 % of participants. Completion rates were also high for blood (99 %), lymphedema (98 %), and questionnaires (95 %) including patient-reported outcomes and correlates of exercise. The first 500 participants in AMBER are an average age of 56 years, 60 % are overweight or obese, and 58 % have disease stage II or III.
Conclusion: Despite the modest recruitment rate and younger age, AMBER has demonstrated that many newly diagnosed breast cancer survivors are willing and able to complete a wide array of sophisticated and physically demanding HRF and PA assessments soon after diagnosis. AMBER is a unique breast cancer survivor cohort that may inform future randomized controlled trials on lifestyle and breast cancer outcomes as well as PA behavior change in breast cancer survivors. Moreover, AMBER may also inform guidelines on PA, sedentary behavior, and HRF for improving breast cancer outcomes and survivorship.
C1 [Courneya, Kerry S.; Bell, Gordon J.; Morielli, Andria R.; Cook, Diane; Voaklander, Stephanie] Univ Alberta, Fac Phys Educ & Recreat, 1-113 Univ Hall, Edmonton, AB T6G 2H9, Canada.
[McNeely, Margaret L.] Univ Alberta, Fac Rehabil Med, Edmonton, AB, Canada.
[Culos-Reed, S. Nicole] Univ Calgary, Fac Kinesiol, Calgary, AB, Canada.
[Vallance, Jeff K.] Athabasca Univ, Fac Hlth Disciplines, Athabasca, AB, Canada.
[Mackey, John R.] Univ Alberta, Fac Med & Dent, Edmonton, AB, Canada.
[Matthews, Charles E.] US Natl Canc Inst, Div Canc Epidemiol & Genet, Bethesda, MD USA.
[MacLaughlin, Sarah; Farris, Megan S.; O'Reilly, Rachel; Friedenreich, Christine M.] Alberta Hlth Serv, Dept Canc Epidemiol & Prevent Res, Calgary, AB, Canada.
RP Courneya, KS (reprint author), Univ Alberta, Fac Phys Educ & Recreat, 1-113 Univ Hall, Edmonton, AB T6G 2H9, Canada.
EM Kerry.courneya@ualberta.ca
FU Canadian Institutes of Health Research; Canada Research Chairs Program;
Alberta Innovates-Health Solutions Population Health Investigator Award;
AIHS Health Senior Scholar Award; Alberta Cancer Foundation
FX This study is funded by a Team Grant from the Canadian Institutes of
Health Research. KSC and JKV are supported by the Canada Research Chairs
Program. JKV is also supported by an Alberta Innovates-Health Solutions
Population Health Investigator Award. CMF is supported by an AIHS Health
Senior Scholar Award and by the Alberta Cancer Foundation Weekend to End
Women's Cancers Breast Cancer Chair.
NR 17
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U1 5
U2 5
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1471-2407
J9 BMC CANCER
JI BMC Cancer
PD JUL 14
PY 2016
VL 16
AR 481
DI 10.1186/s12885-016-2534-4
PG 9
WC Oncology
SC Oncology
GA DR7PQ
UT WOS:000380092600001
PM 27416835
ER
PT J
AU Vandenberg, LN
Agerstrand, M
Beronius, A
Beausoleil, C
Bergman, A
Bero, LA
Bornehag, CG
Boyer, CS
Cooper, GS
Cotgreave, I
Gee, D
Grandjean, P
Guyton, KZ
Hass, U
Heindel, JJ
Jobling, S
Kidd, KA
Kortenkamp, A
Macleod, MR
Martin, OV
Norinder, U
Scheringer, M
Thayer, KA
Toppari, J
Whaley, P
Woodruff, TJ
Ruden, C
AF Vandenberg, Laura N.
Agerstrand, Marlene
Beronius, Anna
Beausoleil, Claire
Bergman, Ake
Bero, Lisa A.
Bornehag, Carl-Gustaf
Boyer, C. Scott
Cooper, Glinda S.
Cotgreave, Ian
Gee, David
Grandjean, Philippe
Guyton, Kathryn Z.
Hass, Ulla
Heindel, Jerrold J.
Jobling, Susan
Kidd, Karen A.
Kortenkamp, Andreas
Macleod, Malcolm R.
Martin, Olwenn V.
Norinder, Ulf
Scheringer, Martin
Thayer, Kristina A.
Toppari, Jorma
Whaley, Paul
Woodruff, Tracey J.
Ruden, Christina
TI A proposed framework for the systematic review and integrated assessment
(SYRINA) of endocrine disrupting chemicals
SO ENVIRONMENTAL HEALTH
LA English
DT Article
DE Endocrine disrupting chemicals; Systematic review; Study evaluation;
Strength of evidence; Weight of evidence; Adverse effect; Endocrine
disrupting activity; Evidence integration; Epidemiology; In vivo
ID EVIDENCE-BASED MEDICINE; ENVIRONMENTAL-HEALTH SCIENCE; RISK-ASSESSMENT;
PUBLIC-HEALTH; FETAL-GROWTH; DEVELOPMENTAL ORIGINS; SCIENTIFIC
STATEMENT; EVALUATION CRITERIA; DISEASE; GUIDE
AB Background: The issue of endocrine disrupting chemicals (EDCs) is receiving wide attention from both the scientific and regulatory communities. Recent analyses of the EDC literature have been criticized for failing to use transparent and objective approaches to draw conclusions about the strength of evidence linking EDC exposures to adverse health or environmental outcomes. Systematic review methodologies are ideal for addressing this issue as they provide transparent and consistent approaches to study selection and evaluation. Objective methods are needed for integrating the multiple streams of evidence (epidemiology, wildlife, laboratory animal, in vitro, and in silico data) that are relevant in assessing EDCs.
Methods: We have developed a framework for the systematic review and integrated assessment (SYRINA) of EDC studies. The framework was designed for use with the International Program on Chemical Safety (IPCS) and World Health Organization (WHO) definition of an EDC, which requires appraisal of evidence regarding 1) association between exposure and an adverse effect, 2) association between exposure and endocrine disrupting activity, and 3) a plausible link between the adverse effect and the endocrine disrupting activity.
Results: Building from existing methodologies for evaluating and synthesizing evidence, the SYRINA framework includes seven steps: 1) Formulate the problem; 2) Develop the review protocol; 3) Identify relevant evidence; 4) Evaluate evidence from individual studies; 5) Summarize and evaluate each stream of evidence; 6) Integrate evidence across all streams; 7) Draw conclusions, make recommendations, and evaluate uncertainties. The proposed method is tailored to the IPCS/WHO definition of an EDC but offers flexibility for use in the context of other definitions of EDCs.
Conclusions: When using the SYRINA framework, the overall objective is to provide the evidence base needed to support decision making, including any action to avoid/minimise potential adverse effects of exposures. This framework allows for the evaluation and synthesis of evidence from multiple evidence streams. Finally, a decision regarding regulatory action is not only dependent on the strength of evidence, but also the consequences of action/inaction, e.g. limited or weak evidence may be sufficient to justify action if consequences are serious or irreversible.
C1 [Agerstrand, Marlene; Bergman, Ake; Ruden, Christina] Stockholm Univ, Dept Environm Sci & Analyt Chem, Stockholm, Sweden.
[Vandenberg, Laura N.] Univ Massachusetts, Amherst Sch Publ Hlth & Hlth Sci, Dept Environm Hlth Sci, Amherst, MA USA.
[Beronius, Anna] Karolinska Inst, Inst Environm Med, Stockholm, Sweden.
[Beausoleil, Claire] ANSES French Agcy Food Environm & Occupat Hlth Sa, Maisons Alfort, France.
[Bergman, Ake; Boyer, C. Scott; Norinder, Ulf] Swedish Toxicol Sci Res Ctr, Sodertalje, Sweden.
[Bero, Lisa A.] Univ Sydney, Charles Perkins Ctr, Sydney, NSW, Australia.
[Bornehag, Carl-Gustaf] Karlstad Univ, Dept Hlth Sci, Karlstad, Sweden.
[Bornehag, Carl-Gustaf] Icahn Sch Med Mt Sinai, New York, NY USA.
[Cooper, Glinda S.] US EPA, Washington, DC USA.
[Cotgreave, Ian] Karolinska Inst, Swedish Toxicol Sci Res Ctr Swetox, Sodertalje, Sweden.
[Gee, David; Jobling, Susan; Kortenkamp, Andreas; Martin, Olwenn V.] Brunel Univ London, Inst Environm Hlth & Societies, Uxbridge, Middx, England.
[Grandjean, Philippe] Univ Southern Denmark, Dept Environm Med, Odense, Denmark.
Int Agcy Res Canc, Lyon, France.
Tech Univ Denmark, Natl Food Inst, Soborg, Denmark.
[Heindel, Jerrold J.] Natl Inst Environm Hlth Sci, Div Extramural Res & Training, Res Triangle Pk, NC USA.
[Kidd, Karen A.] Univ New Brunswick, Dept Biol, St John, NB, Canada.
[Kidd, Karen A.] Univ New Brunswick, Canadian Rivers Inst, St John, NB, Canada.
[Macleod, Malcolm R.] Univ Edinburgh, Ctr Clin Brain Sci, Edinburgh, Midlothian, Scotland.
[Scheringer, Martin] ETH, Inst Chem & Bioengn, Zurich, Switzerland.
[Thayer, Kristina A.] Natl Inst Environm Hlth Sci, Dept Hlth & Human Serv, Div Natl Toxicol Program, NIH, Res Triangle Pk, NC USA.
[Toppari, Jorma] Univ Turku, Turku Univ Hosp, Turku, Finland.
[Whaley, Paul] Univ Lancaster, Lancaster Environm Ctr, Lancaster, England.
[Woodruff, Tracey J.] Univ Calif San Francisco, Sch Med, Program Reprod Hlth & Environm, Oakland, CA USA.
RP Ruden, C (reprint author), Stockholm Univ, Dept Environm Sci & Analyt Chem, Stockholm, Sweden.
EM christina.ruden@aces.su.se
RI jobling, susan/N-9316-2016;
OI Whaley, Paul/0000-0003-4021-0785; Macleod, Malcolm
Robert/0000-0001-9187-9839; Grandjean, Philippe/0000-0003-4046-9658
FU Swedish Foundation for Strategic Environmental Research "Mistra";
National Institute of Environmental Health Sciences of the National
Institutes of Health [K22ES025811]; Clarence Heller Foundation
[A123547]; Passport Foundation; Forsythia Foundation; National Institute
of Environmental Health Sciences [ES018135, ESO22841]; U.S. EPA STAR
grants [RD83467801, RD83543301]; Academy of Finland; Sigrid Juselius;
Danish EPA; Canada Research Chairs program [950-230607]
FX The workshops that supported the writing of this manuscript were funded
by the Swedish Foundation for Strategic Environmental Research "Mistra".
LNV was funded by Award Number K22ES025811 from the National Institute
of Environmental Health Sciences of the National Institutes of Health.
TJW was funded by The Clarence Heller Foundation (A123547), the Passport
Foundation, the Forsythia Foundation, the National Institute of
Environmental Health Sciences (grants ES018135 and ESO22841), and U.S.
EPA STAR grants (RD83467801 and RD83543301). JT was funded by the
Academy of Finland and Sigrid Juselius. UH was funded by the Danish EPA.
KAK was funded by the Canada Research Chairs program grant number
950-230607.
NR 96
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U1 10
U2 27
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1476-069X
J9 ENVIRON HEALTH-GLOB
JI Environ. Health
PD JUL 14
PY 2016
VL 15
AR 74
DI 10.1186/s12940-016-0156-6
PG 19
WC Environmental Sciences; Public, Environmental & Occupational Health
SC Environmental Sciences & Ecology; Public, Environmental & Occupational
Health
GA DR6EG
UT WOS:000379994300001
PM 27412149
ER
PT J
AU Junker, A
Balasubramanian, R
Ciancetta, A
Uliassi, E
Kiselev, E
Martiriggiano, C
Trujillo, K
Mtchedlidze, G
Birdwell, L
Brown, KA
Harden, TK
Jacobson, KA
AF Junker, Anna
Balasubramanian, Ramachandran
Ciancetta, Antonella
Uliassi, Elisa
Kiselev, Evgeny
Martiriggiano, Chiara
Trujillo, Kevin
Mtchedlidze, Giorgi
Birdwell, Leah
Brown, Kyle A.
Harden, T. Kendall
Jacobson, Kenneth A.
TI Structure-Based Design of 3-(4-Aryl-1H-1,2,3-triazol-1-yl)-Biphenyl
Derivatives as P2Y(14) Receptor Antagonists
SO JOURNAL OF MEDICINAL CHEMISTRY
LA English
DT Article
ID GENERAL FORCE-FIELD; MOLECULAR-DYNAMICS; HUMAN NEUTROPHILS; CHEMOTAXIS;
INHIBITION; AUTOMATION; CHEMISTRY; TEMPLATE; LIGANDS; ESTERS
AB UDP and UDP-glucose activate the P2Y(14) receptor (P2Y(14)R) to modulate processes related to inflammation, diabetes, and asthma. A computational pipeline suggested alternatives to naphthalene of a previously reported P2Y14R antagonist (3, PPTN) using docking and molecular dynamics simulations on a hP2Y(14)R homology model based on P2Y(12)R structures. By reevaluating the binding of 3 to P2Y(14)R computationally, two alternatives, i.e., alkynyl and triazolyl derivatives, were identified. Improved synthesis of fluorescent antagonist 4 enabled affinity quantification (IC(50)s, nM) using flow cytometry of P2Y(14)R-expressing CHO cells. p-F3C-phenyl-triazole 65 (32) was more potent than a corresponding alkyne 11. Thus, additional triazolyl derivatives were prepared, as guided by docking simulations, with nonpolar aryl substituents favored. Although triazoles were less potent than 3 (6), simpler synthesis facilitated further structural optimization. Additionally, relative P2Y(14)R affinities agreed with predicted binding of alkynyl and triazole analogues. These triazoles, designed through a structure-based approach, can be assessed in disease models.
C1 [Junker, Anna; Balasubramanian, Ramachandran; Ciancetta, Antonella; Uliassi, Elisa; Kiselev, Evgeny; Martiriggiano, Chiara; Trujillo, Kevin; Mtchedlidze, Giorgi; Birdwell, Leah; Jacobson, Kenneth A.] NIDDK, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA.
[Brown, Kyle A.; Harden, T. Kendall] Univ N Carolina, Dept Pharmacol, Sch Med, Chapel Hill, NC 27599 USA.
RP Jacobson, KA (reprint author), NIDDK, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA.
EM kennethj@niddk.nih.gov
RI Jacobson, Kenneth/A-1530-2009
OI Jacobson, Kenneth/0000-0001-8104-1493
FU NIDDK Intramural Research Program [ZIA DK031116-28]; Deutsche
Forschungsgemeinschaft (German Research Foundation); National Institute
of Mental Health's Psychoactive Drug Screening Program
[HHSN-271-2008-00025-C]
FX We acknowledge support from the NIDDK Intramural Research Program (ZIA
DK031116-28) and technical assistance of Ryan Surujdin and Jinha Yu
(NIDDK). Financial support of Anna Junker by the Deutsche
Forschungsgemeinschaft (German Research Foundation) is gratefully
acknowledged. We thank Bryan L. Roth (University of North Carolina at
Chapel Hill) and National Institute of Mental Health's Psychoactive Drug
Screening Program (contract no. HHSN-271-2008-00025-C) for screening
data. We thank Rolf Swenson and Zhen-Dan Shi (Imaging Probe Development
Center, NHLBI) for reagents 115 and 116.
NR 46
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U1 5
U2 12
PU AMER CHEMICAL SOC
PI WASHINGTON
PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN 0022-2623
EI 1520-4804
J9 J MED CHEM
JI J. Med. Chem.
PD JUL 14
PY 2016
VL 59
IS 13
BP 6149
EP 6168
DI 10.1021/acs.jmedchem.6b00044
PG 20
WC Chemistry, Medicinal
SC Pharmacology & Pharmacy
GA DR6CN
UT WOS:000379989800014
PM 27331270
ER
PT J
AU Ashinsky, BG
Fishbein, KW
Carter, EM
Lin, PC
Pleshko, N
Raggio, CL
Spencer, RG
AF Ashinsky, Beth G.
Fishbein, Kenneth W.
Carter, Erin M.
Lin, Ping-Chang
Pleshko, Nancy
Raggio, Cathleen L.
Spencer, Richard G.
TI Multiparametric Classification of Skin from Osteogenesis Imperfecta
Patients and Controls by Quantitative Magnetic Resonance Microimaging
SO PLOS ONE
LA English
DT Article
ID COLLAGEN MUTATION DATABASE; CONNECTIVE-TISSUE; RELAXATION-TIMES;
CARTILAGE; MRI; AGE; DISEASE; TESLA; T2
AB The purpose of this study is to evaluate the ability of quantitative magnetic resonance imaging (MRI) to discriminate between skin biopsies from individuals with osteogenesis imperfecta (OI) and skin biopsies from individuals without OI. Skin biopsies from nine controls (unaffected) and nine OI patients were imaged to generate maps of five separate MR parameters, T-1, T-2, k(m), MTR and ADC. Parameter values were calculated over the dermal region and used for univariate and multiparametric classification analysis. A substantial degree of overlap of individual MR parameters was observed between control and OI groups, which limited the sensitivity and specificity of univariate classification. Classification accuracies ranging between 39% and 67% were found depending on the variable of investigation, with T-2 yielding the best accuracy of 67%. When several MR parameters were considered simultaneously in a multivariate analysis, the classification accuracies improved up to 89% for specific combinations, including the combination of T-2 and k(m). These results indicate that multiparametric classification by quantitative MRI is able to detect differences between the skin of OI patients and of unaffected individuals, which motivates further study of quantitative MRI for the clinical diagnosis of OI.
C1 [Ashinsky, Beth G.; Fishbein, Kenneth W.; Spencer, Richard G.] NIA, Lab Clin Invest, Magnet Resonance Imaging & Spect Sect, NIH, Baltimore, MD 21224 USA.
[Carter, Erin M.; Raggio, Cathleen L.] Hosp Special Surg, Kathryn O & Alan C Greenberg Ctr Skeletal Dysplas, 535 E 70th St, New York, NY 10021 USA.
[Lin, Ping-Chang] Augusta Univ, GRU Canc Ctr, Core Imaging Facil Small Anim, Augusta, GA USA.
[Pleshko, Nancy] Temple Univ, Coll Engn, Dept Bioengn, Philadelphia, PA 19122 USA.
[Raggio, Cathleen L.] Hosp Special Surg, Dept Orthopaed, 535 E 70th St, New York, NY 10021 USA.
RP Spencer, RG (reprint author), NIA, Lab Clin Invest, Magnet Resonance Imaging & Spect Sect, NIH, Baltimore, MD 21224 USA.
EM spencer@helix.nih.gov
OI Fishbein, Kenneth/0000-0002-6353-4603
FU Pediatric Society of North America; National Institute on Aging,
National Institutes of Health, Intramural Research Program
FX This work was supported by a Pediatric Society of North America
(https://posna.org/) Grant to CLR, "Identification of Skin Abnormalities
in OI Patients by MR Imaging" and the National Institute on Aging,
National Institutes of Health, Intramural Research Program (RGS). The
funders had no role in study design, data collection and analysis,
decision to publish, or preparation of the manuscript.
NR 41
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PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 14
PY 2016
VL 11
IS 7
AR e0157891
DI 10.1371/journal.pone.0157891
PG 14
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR0DZ
UT WOS:000379579500014
PM 27416032
ER
PT J
AU De Vries, RG
Tomlinson, T
Kim, HM
Krenz, C
Haggerty, D
Ryan, KA
Kim, SYH
AF De Vries, Raymond Gene
Tomlinson, Tom
Kim, Hyungjin Myra
Krenz, Chris
Haggerty, Diana
Ryan, Kerry A.
Kim, Scott Y. H.
TI Understanding the Public's Reservations about Broad Consent and
Study-By-Study Consent for Donations to a Biobank: Results of a National
Survey
SO PLOS ONE
LA English
DT Article
ID INFORMED-CONSENT; WILLINGNESS; CANCER; RISK
AB Researchers and policymakers do not agree about the most appropriate way to get consent for the use of donations to a biobank. The most commonly used method is blanket-or broad-consent where donors allow their donation to be used for any future research approved by the biobank. This approach does not account for the fact that some donors may have moral concerns about the uses of their biospecimens. This problem can be avoided using "real-time"-or study-by-study-consent, but this policy places a significant burden on biobanks. In order to better understand the public's preferences regarding biobank consent policy, we surveyed a sample that was representative of the population of the United States. Respondents were presented with 5 biobank consent policies and were asked to indicate which policies were acceptable/unacceptable and to identify the best/worst policies. They were also given 7 research scenarios that could create moral concern (e.g. research intending to make abortions safer and more effective) and asked how likely they would be to provide broad consent knowing that their donation might be used in that research. Substantial minorities found both broad and study-by-study consent to be unacceptable and identified those two options as the worst policies. Furthermore, while the type of moral concern (e.g., regarding abortion, the commercial use of donations, or stem cell research) had no effect on policy preferences, an increase in the number of research scenarios generating moral concerns was related to an increased likelihood of finding broad consent to be the worst policy. The rejection of these ethically problematic and costly extremes is good news for biobanks. The challenge now is to design a policy that combines consent with access to information in a way that assures potential donors that their interests and moral concerns are being respected.
C1 [De Vries, Raymond Gene; Krenz, Chris; Ryan, Kerry A.] Univ Michigan, Ctr Bioeth & Social Sci Med, Ann Arbor, MI 48109 USA.
[Tomlinson, Tom] Michigan State Univ, Ctr Eth & Humanities Life Sci, E Lansing, MI 48824 USA.
[Kim, Hyungjin Myra] Univ Michigan, Ctr Stat Consultat & Res, Ann Arbor, MI 48109 USA.
[Haggerty, Diana] Michigan State Univ, Dept Epidemiol & Biostat, E Lansing, MI 48824 USA.
[Kim, Scott Y. H.] NIH, Ctr Clin, Dept Bioeth, Bldg 10, Bethesda, MD 20892 USA.
[Kim, Scott Y. H.] Univ Michigan, Dept Psychiat, Ann Arbor, MI 48109 USA.
RP De Vries, RG (reprint author), Univ Michigan, Ctr Bioeth & Social Sci Med, Ann Arbor, MI 48109 USA.
EM rdevries@med.umich.edu
OI Krenz, Chris/0000-0002-0514-4586; De Vries, Raymond/0000-0003-3087-3040
FU National Human Genome Research Institute (NHGRI) [5R01HG007172]
FX This research was funded by the National Human Genome Research Institute
(NHGRI), grant number 5R01HG007172. The funder had no role in study
design, data collection and analysis, decision to publish, or
preparation of the manuscript.
NR 30
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U1 1
U2 3
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 14
PY 2016
VL 11
IS 7
AR e0159113
DI 10.1371/journal.pone.0159113
PG 11
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR0DZ
UT WOS:000379579500079
PM 27415017
ER
PT J
AU Wang, TY
Santos, JH
Feng, J
Fargo, DC
Shen, L
Riadi, G
Keeley, E
Rosh, ZS
Nestler, EJ
Woychik, RP
AF Wang, Tianyuan
Santos, Janine H.
Feng, Jian
Fargo, David C.
Shen, Li
Riadi, Gonzalo
Keeley, Elizabeth
Rosh, Zachary S.
Nestler, Eric J.
Woychik, Richard P.
TI A Novel Analytical Strategy to Identify Fusion Transcripts between
Repetitive Elements and Protein Coding-Exons Using RNA-Seq
SO PLOS ONE
LA English
DT Article
ID TRANSPOSABLE ELEMENTS; NUCLEUS-ACCUMBENS; GENE-EXPRESSION; CELLS;
METHYLATION; ISOFORM; TOPHAT; BRAIN
AB Repetitive elements (REs) comprise 40-60% of the mammalian genome and have been shown to epigenetically influence the expression of genes through the formation of fusion transcript (FTs). We previously showed that an intracisternal A particle forms an FT with the agouti gene in mice, causing obesity/type 2 diabetes. To determine the frequency of FTs genome-wide, we developed a TopHat-Fusion-based analytical pipeline to identify FTs with high specificity. We applied it to an RNA-seq dataset from the nucleus accumbens (NAc) of mice repeatedly exposed to cocaine. Cocaine was previously shown to increase the expression of certain REs in this brain region. Using this pipeline that can be applied to single- or paired-end reads, we identified 438 genes expressing 813 different FTs in the NAc. Although all types of studied repeats were present in FTs, simple sequence repeats were underrepresented. Most importantly, reverse-transcription and quantitative PCR validated the expression of selected FTs in an independent cohort of animals, which also revealed that some FTs are the prominent isoforms expressed in the NAc by some genes. In other RNA-seq datasets, developmental expression as well as tissue specificity of some FTs differed from their corresponding non-fusion counterparts. Finally, in silico analysis predicted changes in the structure of proteins encoded by some FTs, potentially resulting in gain or loss of function. Collectively, these results indicate the robustness of our pipeline in detecting these new isoforms of genes, which we believe provides a valuable tool to aid in better understanding the broad role of REs in mammalian cellular biology.
C1 [Wang, Tianyuan; Santos, Janine H.; Fargo, David C.; Woychik, Richard P.] NIEHS, 111 TW Alexander Dr,Bldg 101, Res Triangle Pk, NC 27709 USA.
[Feng, Jian; Shen, Li; Keeley, Elizabeth; Rosh, Zachary S.; Nestler, Eric J.] Icahn Sch Med Mt Sinai, Fishberg Dept Neurosci, One Gustave L Levy Pl,Box 1065, New York, NY 10029 USA.
[Feng, Jian; Shen, Li; Keeley, Elizabeth; Rosh, Zachary S.; Nestler, Eric J.] Icahn Sch Med Mt Sinai, Friedman Brain Inst, One Gustave L Levy Pl,Box 1065, New York, NY 10029 USA.
[Riadi, Gonzalo] Univ Talca, Fac Ingn, Dept Bioinformat, CBSM, Av 2 Norte 685, Talca 3465548, Chile.
RP Woychik, RP (reprint author), NIEHS, 111 TW Alexander Dr,Bldg 101, Res Triangle Pk, NC 27709 USA.; Nestler, EJ (reprint author), Icahn Sch Med Mt Sinai, Fishberg Dept Neurosci, One Gustave L Levy Pl,Box 1065, New York, NY 10029 USA.; Nestler, EJ (reprint author), Icahn Sch Med Mt Sinai, Friedman Brain Inst, One Gustave L Levy Pl,Box 1065, New York, NY 10029 USA.
EM eric.nestler@mssm.edu; rick.woychik@nih.gov
FU Intramural Research Program of the NIH; [P01DA008227]; [Fondecyt
11140869]
FX This research was supported by the Intramural Research Program of the
NIH (RPW) and by grants P01DA008227 (EJN) and Fondecyt 11140869 (GR).
The funders had no role in study design, data collection and analysis,
decision to publish, or preparation of the manuscript.
NR 36
TC 1
Z9 1
U1 0
U2 0
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 14
PY 2016
VL 11
IS 7
AR e0159028
DI 10.1371/journal.pone.0159028
PG 20
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR0DZ
UT WOS:000379579500059
PM 27415830
ER
PT J
AU Davids, MS
Kim, HT
Bachireddy, P
Costello, C
Liguori, R
Savell, A
Lukez, AP
Avigan, D
Chen, YB
McSweeney, P
LeBoeuf, NR
Rooney, MS
Bowden, M
Zhou, CSW
Granter, SR
Hornick, JL
Rodig, SJ
Hirakawa, M
Severgnini, M
Hodi, FS
Wu, CJ
Ho, VT
Cutler, C
Koreth, J
Alyea, EP
Antin, JH
Armand, P
Streicher, H
Ball, ED
Ritz, J
Bashey, A
Soiffer, RJ
AF Davids, Matthew S.
Kim, Haesook T.
Bachireddy, Pavan
Costello, Caitlin
Liguori, Rebecca
Savell, Alexandra
Lukez, Alexander P.
Avigan, David
Chen, Yi-Bin
McSweeney, Peter
LeBoeuf, Nicole R.
Rooney, Michael S.
Bowden, Michaela
Zhou, Chensheng W.
Granter, Scott R.
Hornick, Jason L.
Rodig, Scott J.
Hirakawa, Masahiro
Severgnini, Mariano
Hodi, F. Stephen
Wu, Catherine J.
Ho, Vincent T.
Cutler, Corey
Koreth, John
Alyea, Edwin P.
Antin, Joseph H.
Armand, Philippe
Streicher, Howard
Ball, Edward D.
Ritz, Jerome
Bashey, Asad
Soiffer, Robert J.
CA Leukemia Lymphoma Soc Blood Canc R
TI Ipilimumab for Patients with Relapse after Allogeneic Transplantation
SO NEW ENGLAND JOURNAL OF MEDICINE
LA English
DT Article
ID STEM-CELL TRANSPLANTATION; ACUTE MYELOID-LEUKEMIA; VERSUS-HOST-DISEASE;
HEMATOLOGIC MALIGNANCIES; T-CELLS; BLOCKADE; CTLA-4; PATTERN; HLA
AB BACKGROUND
Loss of donor-mediated immune antitumor activity after allogeneic hematopoietic stem-cell transplantation (HSCT) permits relapse of hematologic cancers. We hypothesized that immune checkpoint blockade established by targeting cytotoxic T-lymphocyte-associated protein 4 with ipilimumab could restore antitumor reactivity through a graft-versus-tumor effect.
METHODS
We conducted a phase 1/1b multicenter, investigator-initiated study to determine the safety and efficacy of ipilimumab in patients with relapsed hematologic cancer after allogeneic HSCT. Patients received induction therapy with ipilimumab at a dose of 3 or 10 mg per kilogram of body weight every 3 weeks for a total of 4 doses, with additional doses every 12 weeks for up to 60 weeks in patients who had a clinical benefit.
RESULTS
A total of 28 patients were enrolled. Immune-related adverse events, including one death, were observed in 6 patients (21%), and graft-versus-host disease (GVHD) that precluded further administration of ipilimumab was observed in 4 patients (14%). No responses that met formal response criteria occurred in patients who received a dose of 3 mg per kilogram. Among 22 patients who received a dose of 10 mg per kilogram, 5 (23%) had a complete response, 2 (9%) had a partial response, and 6 (27%) had decreased tumor burden. Complete responses occurred in 4 patients with extramedullary acute myeloid leukemia and 1 patient with the myelodysplastic syndrome developing into acute myeloid leukemia. Four patients had a durable response for more than 1 year. Responses were associated with in situ infiltration of cytotoxic CD8+ T cells, decreased activation of regulatory T cells, and expansion of subpopulations of effector T cells in the blood.
CONCLUSIONS
Our early-phase data showed that administration of ipilimumab was feasible in patients with recurrent hematologic cancers after allogeneic HSCT, although immune-mediated toxic effects and GVHD occurred. Durable responses were observed in association with several histologic subtypes of these cancers, including extramedullary acute myeloid leukemia.
C1 [Davids, Matthew S.; Kim, Haesook T.; Bachireddy, Pavan; Liguori, Rebecca; Savell, Alexandra; Lukez, Alexander P.; Hirakawa, Masahiro; Severgnini, Mariano; Hodi, F. Stephen; Wu, Catherine J.; Ho, Vincent T.; Cutler, Corey; Koreth, John; Alyea, Edwin P.; Antin, Joseph H.; Armand, Philippe; Ritz, Jerome; Soiffer, Robert J.] Dana Farber Canc Inst, Dept Med Oncol, 450 Brookline Ave, Boston, MA 02115 USA.
[Davids, Matthew S.; Kim, Haesook T.; Bachireddy, Pavan; Liguori, Rebecca; Savell, Alexandra; Lukez, Alexander P.; Avigan, David; Chen, Yi-Bin; Hirakawa, Masahiro; Severgnini, Mariano; Hodi, F. Stephen; Wu, Catherine J.; Ho, Vincent T.; Cutler, Corey; Koreth, John; Alyea, Edwin P.; Antin, Joseph H.; Armand, Philippe; Ritz, Jerome; Soiffer, Robert J.] Harvard Med Sch, Boston, MA USA.
[Avigan, David] Beth Israel Deaconess Med Ctr, Bone Marrow Transplant Program, Boston, MA 02215 USA.
[Chen, Yi-Bin] Massachusetts Gen Hosp, Ctr Canc, Bone Marrow Transplant Program, Boston, MA USA.
[LeBoeuf, Nicole R.] Dana Farber & Brigham & Womens Canc Ctr, Dept Dermatol, Boston, MA USA.
[Granter, Scott R.; Hornick, Jason L.; Rodig, Scott J.] Dana Farber & Brigham & Womens Canc Ctr, Dept Pathol, Boston, MA USA.
[Bowden, Michaela; Zhou, Chensheng W.] Dana Farber Canc Inst, Ctr Mol Oncol Pathol, Boston, MA 02115 USA.
[Bachireddy, Pavan; Wu, Catherine J.] Broad Inst Massachusetts Inst Technol & Harvard, Cambridge, MA USA.
[Rooney, Michael S.] Neon Therapeut, Cambridge, MA USA.
[Costello, Caitlin; Ball, Edward D.] Univ Calif San Diego, Blood & Marrow Transplant Program, Moores Canc Ctr, La Jolla, CA 92093 USA.
[McSweeney, Peter] Colorado Blood Canc Inst, Denver, CO USA.
[Streicher, Howard] NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA.
[Bashey, Asad] Northside Hosp, Blood & Marrow Transplant Grp Georgia, Atlanta, GA USA.
RP Davids, MS (reprint author), Dana Farber Canc Inst, Dept Med Oncol, 450 Brookline Ave, Boston, MA 02115 USA.
EM matthew_davids@dfci.harvard.edu
FU National Institutes of Health
FX Funded by the National Institutes of Health and others;
ClinicalTrials.gov number, NCT01822509.
NR 22
TC 17
Z9 17
U1 3
U2 6
PU MASSACHUSETTS MEDICAL SOC
PI WALTHAM
PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA
SN 0028-4793
EI 1533-4406
J9 NEW ENGL J MED
JI N. Engl. J. Med.
PD JUL 14
PY 2016
VL 375
IS 2
BP 143
EP 153
DI 10.1056/NEJMoa1601202
PG 11
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ9KY
UT WOS:000379530000007
PM 27410923
ER
PT J
AU Panackal, AA
Marr, KA
Williamson, PR
AF Panackal, Anil A.
Marr, Kieren A.
Williamson, Peter R.
TI Dexamethasone in Cryptococcal Meningitis
SO NEW ENGLAND JOURNAL OF MEDICINE
LA English
DT Letter
ID GATTII
C1 [Panackal, Anil A.; Williamson, Peter R.] NIH, Bldg 10, Bethesda, MD 20892 USA.
[Marr, Kieren A.] Johns Hopkins Med, Baltimore, MD USA.
RP Panackal, AA (reprint author), NIH, Bldg 10, Bethesda, MD 20892 USA.
EM anil.panackal@nih.gov
NR 4
TC 0
Z9 0
U1 0
U2 1
PU MASSACHUSETTS MEDICAL SOC
PI WALTHAM
PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA
SN 0028-4793
EI 1533-4406
J9 NEW ENGL J MED
JI N. Engl. J. Med.
PD JUL 14
PY 2016
VL 375
IS 2
BP 188
EP 188
PG 1
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ9KY
UT WOS:000379530000022
PM 27410935
ER
PT J
AU Perkins, TA
Paz-Soldan, VA
Stoddard, ST
Morrison, AC
Forshey, BM
Long, KC
Halsey, ES
Kochel, TJ
Elder, JP
Kitron, U
Scott, TW
Vazquez-Prokopec, GM
AF Perkins, T. Alex
Paz-Soldan, Valerie A.
Stoddard, Steven T.
Morrison, Amy C.
Forshey, Brett M.
Long, Kanya C.
Halsey, Eric S.
Kochel, Tadeusz J.
Elder, John P.
Kitron, Uriel
Scott, Thomas W.
Vazquez-Prokopec, Gonzalo M.
TI Calling in sick: impacts of fever on infra-urban human mobility
SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
LA English
DT Article
DE activity space; contact; dengue; infection; movement; network
ID DENGUE VIRUS TRANSMISSION; HUMAN MOVEMENT; HUMAN-BEHAVIOR; INFECTIONS;
IQUITOS; PERU; HYPOTHESIS; INFLUENZA; PATTERNS; MALARIA
AB Pathogens inflict a wide variety of disease manifestations on their hosts, yet the impacts of disease on the behaviour of infected hosts are rarely studied empirically and are seldom accounted for in mathematical models of transmission dynamics. We explored the potential impacts of one of the most common disease manifestations, fever, on a key determinant of pathogen transmission, host mobility, in residents of the Amazonian city of Iquitos, Peru. We did so by comparing two groups of febrile individuals (dengue positive and dengue-negative) with an afebrile control group. A retrospective, semi-structured interview allowed us to quantify multiple aspects of mobility during the two-week period preceding each interview. We fitted nested models of each aspect of mobility to data from interviews and compared models using likelihood ratio tests to determine whether there were statistically distinguishable differences in mobility attributable to fever or its aetiology. Compared with afebrile individuals, febrile study participants spent more time at home, visited fewer locations, and, in some cases, visited locations closer to home and spent less time at certain types of locations. These multifaceted impacts are consistent with the possibility that disease mediated changes in host mobility generate dynamic and complex changes in host contact network structure.
C1 [Perkins, T. Alex] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA.
[Perkins, T. Alex] Univ Notre Dame, Eck Inst Global Hlth, Notre Dame, IN 46556 USA.
[Perkins, T. Alex; Stoddard, Steven T.; Morrison, Amy C.; Long, Kanya C.; Scott, Thomas W.] Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA.
[Perkins, T. Alex; Kitron, Uriel; Scott, Thomas W.; Vazquez-Prokopec, Gonzalo M.] NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA.
[Paz-Soldan, Valerie A.] Tulane Univ, Sch Publ Hlth & Trop Med, Dept Global Hlth Syst & Dev, New Orleans, LA USA.
[Paz-Soldan, Valerie A.] Univ Peruana Cayetano Heredia, Fac Salud Publ & Adm, Lima, Peru.
[Morrison, Amy C.; Forshey, Brett M.; Halsey, Eric S.; Kochel, Tadeusz J.] US Naval Med Res Unit No 6, Lima, Peru.
[Long, Kanya C.] Andrews Univ, Dept Biol, Berrien Springs, MI 49104 USA.
[Elder, John P.] San Diego State Univ, Grad Sch Publ Hlth, Inst Behav & Community Hlth, San Diego, CA 92182 USA.
[Kitron, Uriel; Vazquez-Prokopec, Gonzalo M.] Emory Univ, Dept Environm Sci, Atlanta, GA 30322 USA.
RP Perkins, TA (reprint author), Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA.; Perkins, TA (reprint author), Univ Notre Dame, Eck Inst Global Hlth, Notre Dame, IN 46556 USA.; Perkins, TA (reprint author), Univ Calif Davis, Dept Entomol & Nematol, Davis, CA 95616 USA.; Perkins, TA (reprint author), NIH, Fogarty Int Ctr, Bldg 10, Bethesda, MD 20892 USA.
EM taperkins@nd.edu
FU US National Institutes of Health-National Institute of Allergy and
Infectious Diseases (NIFI/NIAID) [R01 AI069341-01, 1P01AI098670-01A1];
Research and Policy for Infectious Disease Dynamics (RAPIDD) program of
the Science and Technology Directory, Department of Homeland Security,
and Fogarty International Center, National Institutes of Health
FX This research was supported by grants from the US National Institutes of
Health-National Institute of Allergy and Infectious Diseases
(NIFI/NIAID) awards R01 AI069341-01 and 1P01AI098670-01A1 (to T.W.S.)
and by the Research and Policy for Infectious Disease Dynamics (RAPIDD)
program of the Science and Technology Directory, Department of Homeland
Security, and Fogarty International Center, National Institutes of
Health. Authors E.S.H. and T.J.K. were US Military service members. This
work was prepared as part of their official duties. Title 17 U.S.C. 105
provides that 'Copyright protection under this title is not available
for any work of the United States Government.' Title 17 U.S.C. 101
defines a US Government work as a work prepared by military service
members or employees of the US Government as part of those persons'
official duties.
NR 46
TC 0
Z9 0
U1 7
U2 7
PU ROYAL SOC
PI LONDON
PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND
SN 0962-8452
EI 1471-2954
J9 P ROY SOC B-BIOL SCI
JI Proc. R. Soc. B-Biol. Sci.
PD JUL 13
PY 2016
VL 283
IS 1834
AR 20160390
DI 10.1098/rspb.2016.0390
PG 9
WC Biology; Ecology; Evolutionary Biology
SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences &
Ecology; Evolutionary Biology
GA DT7FI
UT WOS:000381652100009
ER
PT J
AU Sheeler, CA
AF Sheeler, Carrie A.
TI The Influence of Systemic Immune Response and Sleep Modulation on the
Secondary Effects of Traumatic Brain Injury in the Rodent Model
SO JOURNAL OF NEUROSCIENCE
LA English
DT Editorial Material
ID BARRIER; INFLAMMATION; HUMANS; REFLEX
C1 [Sheeler, Carrie A.] NIMH, Sect Neuroadaptat & Prot Metab, Bldg 10,Room 2D54,10 Ctr Dr, Bethesda, MD 20892 USA.
RP Sheeler, CA (reprint author), NIMH, Sect Neuroadaptat & Prot Metab, Bldg 10,Room 2D54,10 Ctr Dr, Bethesda, MD 20892 USA.
EM carrie.sheeler@nih.gov
NR 16
TC 1
Z9 1
U1 3
U2 3
PU SOC NEUROSCIENCE
PI WASHINGTON
PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA
SN 0270-6474
J9 J NEUROSCI
JI J. Neurosci.
PD JUL 13
PY 2016
VL 36
IS 28
BP 7341
EP 7342
DI 10.1523/JNEUROSCI.1418-16.2016
PG 2
WC Neurosciences
SC Neurosciences & Neurology
GA DS1ST
UT WOS:000380380500001
PM 27413144
ER
PT J
AU Aflaki, E
Borger, DK
Moaven, N
Stubblefield, BK
Rogers, SA
Patnaik, S
Schoenen, FJ
Westbroek, W
Zheng, W
Sullivan, P
Fujiwara, H
Sidhu, R
Khaliq, ZM
Lopez, GJ
Goldstein, DS
Ory, DS
Marugan, J
Sidransky, E
AF Aflaki, Elma
Borger, Daniel K.
Moaven, Nima
Stubblefield, Barbara K.
Rogers, Steven A.
Patnaik, Samarjit
Schoenen, Frank J.
Westbroek, Wendy
Zheng, Wei
Sullivan, Patricia
Fujiwara, Hideji
Sidhu, Rohini
Khaliq, Zayd M.
Lopez, Grisel J.
Goldstein, David S.
Ory, Daniel S.
Marugan, Juan
Sidransky, Ellen
TI A New Glucocerebrosidase Chaperone Reduces alpha-Synuclein and
Glycolipid Levels in iPSC-Derived Dopaminergic Neurons from Patients
with Gaucher Disease and Parkinsonism
SO JOURNAL OF NEUROSCIENCE
LA English
DT Article
DE alpha-synuclein; dopaminergic neurons; glucocerebrosidase; induced
pluripotent stem cells; parkinsonism; pharmacological chaperone
ID ENZYME REPLACEMENT THERAPY; MUTATIONS; CELLS; MANIFESTATIONS;
MULTICENTER; DEFICIENCY; CARRIERS
AB Among the known genetic risk factors for Parkinson disease, mutations in GBA1, the gene responsible for the lysosomal disorder Gaucher disease, are the most common. This genetic link has directed attention to the role of the lysosome in the pathogenesis of parkinsonism. To study how glucocerebrosidase impacts parkinsonism and to evaluate new therapeutics, we generated induced human pluripotent stem cells from four patients with Type 1 (non-neuronopathic) Gaucher disease, two with and two without parkinsonism, and one patient with Type 2 (acute neuronopathic) Gaucher disease, and differentiated them into macrophages and dopaminergic neurons. These cells exhibited decreased glucocerebrosidase activity and stored the glycolipid substrates glucosylceramide and glucosylsphingosine, demonstrating their similarity to patients with Gaucher disease. Dopaminergic neurons from patients with Type 2 and Type 1 Gaucher disease with parkinsonism had reduced dopamine storage and dopamine transporter reuptake. Levels of alpha-synuclein, a protein present as aggregates in Parkinson disease and related synucleinopathies, were selectively elevated in neurons from the patients with parkinsonism or Type 2 Gaucher disease. The cells were then treated with NCGC607, a small-molecule noninhibitory chaperone of glucocerebrosidase identified by high-throughput screening and medicinal chemistry structure optimization. This compound successfully chaperoned the mutant enzyme, restored glucocerebrosidase activity and protein levels, and reduced glycolipid storage in both iPSC-derived macrophages and dopaminergic neurons, indicating its potential for treating neuronopathic Gaucher disease. In addition, NCGC607 reduced alpha-synuclein levels in dopaminergic neurons from the patients with parkinsonism, suggesting that noninhibitory small-molecule chaperones of glucocerebrosidase may prove useful for the treatment of Parkinson disease.
C1 [Aflaki, Elma; Borger, Daniel K.; Moaven, Nima; Stubblefield, Barbara K.; Westbroek, Wendy; Lopez, Grisel J.; Sidransky, Ellen] NHGRI, Sect Mol Neurogenet, NIH, Bethesda, MD 20892 USA.
[Patnaik, Samarjit; Zheng, Wei; Marugan, Juan] NINDS, Natl Ctr Adv Translat Sci, NIH, Bethesda, MD 20892 USA.
[Sullivan, Patricia; Goldstein, David S.] NINDS, Clin Neurocardiol Sect, NIH, Bethesda, MD 20892 USA.
[Khaliq, Zayd M.] NINDS, Cellular Neurophysiol Unit, NIH, Bethesda, MD 20892 USA.
[Rogers, Steven A.; Schoenen, Frank J.] Univ Kansas, Specialized Chem Ctr, Lawrence, KS 66047 USA.
[Fujiwara, Hideji; Sidhu, Rohini; Ory, Daniel S.] Washington Univ, Sch Med, Diabet Cardiovasc Dis Ctr, St Louis, MO 63110 USA.
[Fujiwara, Hideji; Sidhu, Rohini; Ory, Daniel S.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA.
RP Sidransky, E (reprint author), NHGRI, Sect Mol Neurogenet, Med Genet Branch, NIH, Bldg 35,Room 1E623,35 Convent Dr,MSC 3708, Bethesda, MD 20892 USA.
EM sidranse@mail.nih.gov
RI Sidhu, Rohini/G-3547-2012; Zheng, Wei/J-8889-2014
OI Zheng, Wei/0000-0003-1034-0757
FU Intramural Research Programs of National Human Genome Research
Institute, National Center for Advancing Translational Sciences,
National Institute of Neurological Disorders and Stroke,; National Human
Genome Research Institute [U54HG005031]; National Institutes of Health
FX This work was supported by the Intramural Research Programs of the
National Human Genome Research Institute, National Center for Advancing
Translational Sciences, National Institute of Neurological Disorders and
Stroke, and National Institutes of Health and in part by National Human
Genome Research Institute Grant U54HG005031.
NR 34
TC 9
Z9 9
U1 9
U2 12
PU SOC NEUROSCIENCE
PI WASHINGTON
PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA
SN 0270-6474
J9 J NEUROSCI
JI J. Neurosci.
PD JUL 13
PY 2016
VL 36
IS 28
BP 7441
EP 7452
DI 10.1523/JNEUROSCI.0636-16.2016
PG 12
WC Neurosciences
SC Neurosciences & Neurology
GA DS1ST
UT WOS:000380380500011
PM 27413154
ER
PT J
AU Smith, AA
Navasa, N
Yang, XL
Wilder, CN
Buyuktanir, O
Marques, A
Anguita, J
Pal, U
AF Smith, Alexis A.
Navasa, Nicolas
Yang, Xiuli
Wilder, Cara N.
Buyuktanir, Ozlem
Marques, Adriana
Anguita, Juan
Pal, Utpal
TI Cross-Species Interferon Signaling Boosts Microbicidal Activity within
the Tick Vector
SO CELL HOST & MICROBE
LA English
DT Article
ID LYME-DISEASE SPIROCHETE; IXODES-SCAPULARIS; BORRELIA-BURGDORFERI;
ANTIMICROBIAL PEPTIDES; STAT PROTEINS; IMMUNITY; DROSOPHILA; JAK;
PATHWAY; GENES
AB Evolution of hematophagy in blood-sucking parasites likely involves communication with their hosts. We find that Ixodes ticks are responsive to IFNg acquired in a blood meal from mice infected with the Lyme disease-causing bacteria Borrelia burgdorferi, leading to induction of antimicrobial responses. Ixodes ticks parasitizing B. burgdorferi-infected mice upregulated an I. scapularis Rho-like GTPase (IGTPase). IGTPase knockdown enhanced B. burgdorferi levels in postfed ticks, suggesting this protein controls spirochete survival. Notably, IGTPase was only induced during pathogen acquisition from mice and not upon transmission to naive hosts. Microinjection of ticks with IFNg induced IGTPase, and ticks parasitizing IFNg knockout mice, failed to upregulate IGTPase. Additionally, ticks lacking the transcription factor STAT, which signals downstream of IFNg, did not induce IGTPase. IGTPase expression induced antimicrobial peptides, including Dae2, previously shown to inhibit B. burgdorferi. These results identify an interspecies signaling cascade allowing ticks to detect invading bacteria and mount microbicidal responses.
C1 [Smith, Alexis A.; Yang, Xiuli; Wilder, Cara N.; Pal, Utpal] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA.
[Navasa, Nicolas; Anguita, Juan] CIC BioGUNE, Derio 48160, Bizkaia, Spain.
[Buyuktanir, Ozlem] Ondokuz Mayis Univ, Dept Microbiol, Fac Vet Med, TR-55139 Samsun, Turkey.
[Marques, Adriana] NIAID, Lab Clin Infect Dis, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Anguita, Juan] Basque Fdn Sci, Ikerbasque, Bilbao 48011, Spain.
[Pal, Utpal] Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA.
RP Pal, U (reprint author), Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA.; Pal, U (reprint author), Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA.
EM upal@umd.edu
RI Anguita, Juan/D-5432-2011
OI Anguita, Juan/0000-0003-2061-7182
FU University of Maryland, College Park; National Institute of Allergy and
Infectious Diseases [AI080615, AI116620]; Ondokuz Mayis University;
Turkish Higher Education Council; Intramural Research Program of the
National Institute of Allergy and Infectious Disease; European Union
[602272]
FX We thank John Anderson, Toru Kariu, Faith Kung, Kavita Sharma, Brian
Backstedt, Juraj Koci, and Meghna Thakur for their assistance with this
study. We would like to thank Ulrike Munderloh, University of Minnesota,
for providing the IDE12 cell line. This work was supported by funding
from University of Maryland, College Park, and the National Institute of
Allergy and Infectious Diseases Award Number AI080615 and AI116620 (to
U.P.). O.B. is supported by funding from Ondokuz Mayis University and
Turkish Higher Education Council. A.M. is supported by the Intramural
Research Program of the National Institute of Allergy and Infectious
Disease. J.A. is funded by the European Union (Grant Agreement number
602272).
NR 27
TC 6
Z9 6
U1 2
U2 7
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 1931-3128
EI 1934-6069
J9 CELL HOST MICROBE
JI Cell Host Microbe
PD JUL 13
PY 2016
VL 20
IS 1
BP 91
EP 98
DI 10.1016/j.chom.2016.06.001
PG 8
WC Microbiology; Parasitology; Virology
SC Microbiology; Parasitology; Virology
GA DS3UC
UT WOS:000380707200013
PM 27374407
ER
PT J
AU Cho, YE
Latour, LL
Kim, H
Turtzo, LC
Olivera, A
Livingston, WS
Wang, D
Martin, C
Lai, C
Cashion, A
Gill, J
AF Cho, Young-Eun
Latour, Lawrence L.
Kim, Hyungsuk
Turtzo, L. Christine
Olivera, Anlys
Livingston, Whitney S.
Wang, Dan
Martin, Christiana
Lai, Chen
Cashion, Ann
Gill, Jessica
TI Older Age Results in Differential Gene Expression after Mild Traumatic
Brain Injury and Is Linked to Imaging Differences at Acute Follow-up
SO FRONTIERS IN AGING NEUROSCIENCE
LA English
DT Article
DE traumatic brain injury; aging; inflammation; gene expression; imaging
ID ACTIVATION; DISEASE; CELLS; TRANSCRIPTOME; INFLAMMATION; METAANALYSIS;
MICROARRAY; PREDICTION; RECOVERY; CORTEX
AB Older age consistently relates to a lesser ability to fully recover from a traumatic brain injury (TBI); however, there is limited data to explicate the nature of age-related risks. This study was undertaken to determine the relationship of age on gene-activity following a TBI, and how this biomarker relates to changes in neuroimaging findings. A young group (between the ages of 19 and 35 years), and an old group (between the ages of 60 and 89 years) were compared on global gene-activity within 48 h following a TBI, and then at follow-up within 1-week. At each time-point, gene expression profiles, and imaging findings from both magnetic resonance imaging (MRI) and computed tomography were obtained and compared. The young group was found to have greater gene expression of inflammatory regulatory genes at 48 h and 1-week in genes such as basic leucine zipper transcription factor 2 (BACH2), leucine-rich repeat neuronal 3 (LRRN3), and lymphoid enhancer-binding factor 1 (LEF1) compared to the old group. In the old group, there was increased activity in genes within S100 family, including calcium binding protein P (S100P) and S100 calcium binding protein A8 (S100A8), which previous studies have linked to poor recovery from TBI. The old group also had reduced activity of the noggin (NOG) gene, which is a member of the transforming growth factorT supertamily and is linked to neurorecovery and neuroregeneration compared to the young group. We link these gene expression findings that were validated to neuroimaging, reporting that in the old group with a MRI finding of TBI-related damage, there was a lesser likelihood to then have a negative MRI finding at follow-up compared to the young group. Together, these data indicate that age impacts gene activity following a TBI, and suggest that this differential activity related to immune regulation and neurorecovery contributes to a lesser likelihood of neuronal recovery in older patients as indicated through neuroimaging.
C1 [Cho, Young-Eun; Kim, Hyungsuk; Olivera, Anlys; Livingston, Whitney S.; Wang, Dan; Martin, Christiana; Lai, Chen; Cashion, Ann; Gill, Jessica] NINR, NIH, Bethesda, MD 20892 USA.
[Latour, Lawrence L.; Turtzo, L. Christine] Natl Inst Neurol Disorders, NIH, Bethesda, MD USA.
RP Cashion, A; Gill, J (reprint author), NINR, NIH, Bethesda, MD 20892 USA.
EM ann.cashion@nih.gov; gillj@mail.nih.gov
OI Martin, Christiana/0000-0002-4484-6134
FU Intramural Research Program of National Institute of Nursing Research in
NIH; Center for Neuroscience and Regenerative Medicine
FX This research was supported by the Intramural Research Program of
National Institute of Nursing Research in NIH. This research was also
supported by the Center for Neuroscience and Regenerative Medicine.
NR 66
TC 0
Z9 0
U1 2
U2 2
PU FRONTIERS MEDIA SA
PI LAUSANNE
PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015,
SWITZERLAND
SN 1663-4365
J9 FRONT AGING NEUROSCI
JI Front. Aging Neurosci.
PD JUL 13
PY 2016
VL 8
AR 168
DI 10.3389/fnagi.2016.00168
PG 11
WC Geriatrics & Gerontology; Neurosciences
SC Geriatrics & Gerontology; Neurosciences & Neurology
GA DR6XH
UT WOS:000380044100001
PM 27468266
ER
PT J
AU Fenwick, RB
Schwieters, CD
Vogeli, B
AF Fenwick, R. Bryn
Schwieters, Charles D.
Vogeli, Beat
TI Direct Investigation of Slow Correlated Dynamics in Proteins via Dipolar
Interactions
SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
LA English
DT Article
ID SMALL ALPHA/BETA PROTEIN; RESIDUAL DIPOLAR; CROSS-CORRELATION;
NMR-SPECTROSCOPY; SUCCESSIVE RESIDUES; MOLECULAR-DYNAMICS; BACKBONE
DYNAMICS; RELAXATION RATES; SCALAR COUPLINGS; SPIN RELAXATION
AB The synchronization of native state motions as they transition between microstates influences catalysis kinetics, mediates allosteric interactions, and reduces the conformational entropy of proteins. However, it has proven difficult to describe native microstates because they are usually minimally frustrated and may interconvert on the micro- to millisecond time scale. Direct observation of concerted equilibrium fluctuations would therefore be an important tool for describing protein native states. Here we propose a strategy that relates NMR cross-correlated relaxation (CCR) rates between dipolar interactions to residual dipolar couplings (RDCs) of individual consecutive H-N-N and H-alpha-C-alpha bonds, which act as a proxy for the peptide planes and the side chains, respectively. Using Xplor-NIH ensemble structure calculations restrained with the RDC and CCR data, we observe collective motions on time scales slower than nanoseconds in the backbone for GB3. To directly access the correlations from CCR, we develop a structure-free data analysis. The resulting dynamic correlation map is consistent with the ensemble-restrained simulations and reveals a complex network. In general, we find that the bond motions are on average slightly correlated and that the local environment dominates many observations. Despite this, some patterns are typical over entire secondary structure elements. In the beta-sheet, nearly all bonds are weakly correlated, and there is an approximately binary alternation in correlation intensity corresponding to the solvent exposure/shielding alternation of the side chains. For alpha-helices, there is also a weak correlation in the H-N-N bonds. The degree of correlation involving H-alpha-C-alpha bonds is directly affected by side-chain fluctuations, whereas loops show complex and nonuniform behavior.
C1 [Fenwick, R. Bryn] Inst Res Biomed IRB Barcelona, Parc Cient Barcelona,C Baldiri Reixac 10, Barcelona 08028, Spain.
[Fenwick, R. Bryn] Scripps Res Inst TSRI, 10550 North Torrey Pines Rd, La Jolla, CA 92037 USA.
[Schwieters, Charles D.] NIH, Div Computat Biosci, Ctr Informat Technol, Bldg 12A, Bethesda, MD 20892 USA.
[Vogeli, Beat] ETH Honggerberg, Swiss Fed Inst Technol, Phys Chem Lab, Vladimir Prelog Weg 2, CH-8093 Zurich, Switzerland.
RP Fenwick, RB (reprint author), Inst Res Biomed IRB Barcelona, Parc Cient Barcelona,C Baldiri Reixac 10, Barcelona 08028, Spain.; Fenwick, RB (reprint author), Scripps Res Inst TSRI, 10550 North Torrey Pines Rd, La Jolla, CA 92037 USA.; Vogeli, B (reprint author), ETH Honggerberg, Swiss Fed Inst Technol, Phys Chem Lab, Vladimir Prelog Weg 2, CH-8093 Zurich, Switzerland.
EM fenwick@scripps.edu; beat.voegeli@phys.chem.ethz.ch
OI Fenwick, Robert/0000-0002-0925-7422
FU Swiss National Science Foundation [140214]; Intramural Research Program
of C.I.T, National Institutes of Health
FX We thank Prof. Roland Riek, Dr. Jason Greenwald, Dr. Michael Sabo, and
Dr. Donghan Lee for valuable discussion. We note that a similar
correlation factor Fcorr has been presented by Dr. Donghan
Lee at the Swiss Federal Institute of Technology and Dr. Michael Sabo at
the 54th ENC conference 2013 in Asilomar. This work was supported by the
Swiss National Science Foundation with Grant 140214 to B.V. and by the
Intramural Research Program of C.I.T, National Institutes of Health, to
C.D.S.
NR 72
TC 0
Z9 0
U1 17
U2 17
PU AMER CHEMICAL SOC
PI WASHINGTON
PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN 0002-7863
J9 J AM CHEM SOC
JI J. Am. Chem. Soc.
PD JUL 13
PY 2016
VL 138
IS 27
BP 8412
EP 8421
DI 10.1021/jacs.6b01447
PG 10
WC Chemistry, Multidisciplinary
SC Chemistry
GA DR3HN
UT WOS:000379794400024
PM 27331619
ER
PT J
AU Bayro, MJ
Tycko, R
AF Bayro, Marvin J.
Tycko, Robert
TI Structure of the Dimerization Interface in the Mature HIV-1 Capsid
Protein Lattice from Solid State NMR of Tubular Assemblies
SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
LA English
DT Article
ID HUMAN-IMMUNODEFICIENCY-VIRUS; NUCLEAR-MAGNETIC-RESONANCE;
CRYSTAL-STRUCTURE; MAS NMR; SPECTROSCOPY; DOMAIN; DYNAMICS;
CONFORMATIONS; SIMULATION; FRAGMENT
AB The HIV-1 capsid protein (CA) forms the capsid shell that encloses RNA within a mature HIV-1 virion. Previous studies by electron microscopy have shown that the capsid shell is primarily a triangular lattice of CA hexamers, with variable curvature that destroys the ideal symmetry of a planar lattice. The mature CA lattice depends on CA dimerization, which occurs through interactions between helix 9 segments of the C-terminal domain (CTD) of CA. Several high-resolution structures of the CTD-CTD dimerization interface have been reported, based on X-ray crystallography and multidimensional solution nuclear magnetic resonance (NMR), with significant differences in amino acid side chain conformations and helix 9-helix 9 orientations. In a structural model for tubular CA assemblies based on cryogenic electron microscopy (cryoEM) [Zhao et al. Nature, 2013, 497, 643-646], the dimerization interface is substantially disordered. The dimerization interface structure in noncrystalline CA assemblies and the extent to which this interface is structurally ordered within a curved lattice have therefore been unclear. Here we describe solid state NMR measurements on the dimerization interface in tubular CA assemblies, which contain the curved triangular lattice of a mature virion, including quantitative measurements of intermolecular and intramolecular distances using dipolar recoupling techniques, solid state NMR chemical shifts, and long-range side chain-side chain contacts. When combined with restraints on the distance and orientation between helix 9 segments from the cryoEM study, the solid state NMR data lead to a unique high-resolution structure for the dimerization interface in the noncrystalline lattice of CA tubes. These results demonstrate that CA lattice curvature is not dependent on disorder or variability in the dimerization interface. This work also demonstrates the feasibility of local structure determination within large noncrystalline assemblies formed by high-molecular weight proteins, using modern solid state NMR methods.
C1 [Bayro, Marvin J.; Tycko, Robert] Natl Inst Diabet & Digest & Kidney Dis, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
RP Tycko, R (reprint author), Natl Inst Diabet & Digest & Kidney Dis, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
EM robertty@mail.nih.gov
RI Bayro, Marvin/Q-4643-2016
OI Bayro, Marvin/0000-0003-1482-9381
FU Intramural Research Program of the National Institute of Diabetes and
Digestive and Kidney Diseases of the National Institutes of Health; NIH
Intramural AIDS Targeted Antiviral Program
FX This work was supported by the Intramural Research Program of the
National Institute of Diabetes and Digestive and Kidney Diseases of the
National Institutes of Health, and by the NIH Intramural AIDS Targeted
Antiviral Program. We thank Dr. Eric Moore for assistance with
simulations of NMR data and Drs. Charles Schwieters and Guillermo
Bermejo for assistance with Xplor-NIH calculations.
NR 46
TC 2
Z9 2
U1 11
U2 23
PU AMER CHEMICAL SOC
PI WASHINGTON
PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN 0002-7863
J9 J AM CHEM SOC
JI J. Am. Chem. Soc.
PD JUL 13
PY 2016
VL 138
IS 27
BP 8538
EP 8546
DI 10.1021/jacs.6b03983
PG 9
WC Chemistry, Multidisciplinary
SC Chemistry
GA DR3HN
UT WOS:000379794400038
PM 27298207
ER
PT J
AU Darbinyan, A
Major, EO
Morgello, S
Holland, S
Ryschkewitsch, C
Monaco, MC
Naidich, TP
Bederson, J
Malaczynska, J
Ye, F
Gordon, R
Cunningham-Rundles, C
Fowkes, M
Tsankova, NM
AF Darbinyan, Armine
Major, Eugene O.
Morgello, Susan
Holland, Steven
Ryschkewitsch, Caroline
Monaco, Maria Chiara
Naidich, Thomas P.
Bederson, Joshua
Malaczynska, Joanna
Ye, Fei
Gordon, Ronald
Cunningham-Rundles, Charlotte
Fowkes, Mary
Tsankova, Nadejda M.
TI BK virus encephalopathy and sclerosing vasculopathy in a patient with
hypohidrotic ectodermal dysplasia and immunodeficiency
SO ACTA NEUROPATHOLOGICA COMMUNICATIONS
LA English
DT Article
DE Polyomavirus; BK virus; IKK-gamma; NF-kappa-B essential modulator
(NEMO); Ectodermal dysplasia; HED-ID; Encephalopathy; Fibrosing
vasculopathy
ID PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY; NONCODING CONTROL REGION;
CENTRAL-NERVOUS-SYSTEM; INCONTINENTIA PIGMENTI; JC VIRUS; POLYOMAVIRUS
BK; TRANSPLANT RECIPIENTS; PRODUCTIVE INFECTION; CEREBROSPINAL-FLUID;
FUNCTIONAL-ANALYSIS
AB Human BK polyomavirus (BKV) is reactivated under conditions of immunosuppression leading most commonly to nephropathy or cystitis; its tropism for the brain is rare and poorly understood. We present a unique case of BKV-associated encephalopathy in a man with hypohidrotic ectodermal dysplasia and immunodeficiency (HED-ID) due to IKK-gamma (NEMO) mutation, who developed progressive neurological symptoms. Brain biopsy demonstrated polyomavirus infection of gray and white matter, with predominant involvement of cortex and distinct neuronal tropism, in addition to limited demyelination and oligodendroglial inclusions. Immunohistochemistry demonstrated polyoma T-antigen in neurons and glia, but expression of VP1 capsid protein only in glia. PCR analysis on both brain biopsy tissue and cerebrospinal fluid detected high levels of BKV DNA. Sequencing studies further identified novel BKV variant and disclosed unique rearrangements in the noncoding control region of the viral DNA (BKVN NCCR). Neuropathological analysis also demonstrated an unusual form of obliterative fibrosing vasculopathy in the subcortical white matter with abnormal lysosomal accumulations, possibly related to the patient's underlying ectodermal dysplasia. Our report provides the first neuropathological description of HED-ID due to NEMO mutation, and expands the diversity of neurological presentations of BKV infection in brain, underscoring the importance of its consideration in immunodeficient patients with unexplained encephalopathy. We also document novel BKVN NCCR rearrangements that may be associated with the unique neuronal tropism in this patient.
C1 [Darbinyan, Armine; Morgello, Susan; Malaczynska, Joanna; Ye, Fei; Gordon, Ronald; Fowkes, Mary; Tsankova, Nadejda M.] Icahn Sch Med Mt Sinai, Dept Pathol, New York, NY 10029 USA.
[Major, Eugene O.; Ryschkewitsch, Caroline; Monaco, Maria Chiara] NINDS, Lab Mol Med & Neurosci, NIH, Bethesda, MD 20892 USA.
[Morgello, Susan] Icahn Sch Med Mt Sinai, Dept Neurol, New York, NY 10029 USA.
[Morgello, Susan; Tsankova, Nadejda M.] Icahn Sch Med Mt Sinai, Dept Neurosci, 1425 Madison Ave,Icahn 9-20E, New York, NY 10029 USA.
[Morgello, Susan; Tsankova, Nadejda M.] Icahn Sch Med Mt Sinai, Friedman Brain Inst, 1425 Madison Ave,Icahn 9-20E, New York, NY 10029 USA.
[Holland, Steven] NIAID, Lab Clin Infect Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Naidich, Thomas P.] Icahn Sch Med Mt Sinai, Dept Radiol, New York, NY 10029 USA.
[Bederson, Joshua] Icahn Sch Med Mt Sinai, Dept Neurosurg, New York, NY 10029 USA.
[Cunningham-Rundles, Charlotte] Icahn Sch Med Mt Sinai, Dept Med Allergy & Immunol, New York, NY 10029 USA.
RP Tsankova, NM (reprint author), Icahn Sch Med Mt Sinai, Dept Pathol, New York, NY 10029 USA.; Tsankova, NM (reprint author), Icahn Sch Med Mt Sinai, Dept Neurosci, 1425 Madison Ave,Icahn 9-20E, New York, NY 10029 USA.; Tsankova, NM (reprint author), Icahn Sch Med Mt Sinai, Friedman Brain Inst, 1425 Madison Ave,Icahn 9-20E, New York, NY 10029 USA.
EM nadejda.tsankova@mssm.edu
NR 59
TC 0
Z9 0
U1 1
U2 1
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 2051-5960
J9 ACTA NEUROPATHOL COM
JI Acta Neuropathol. Commun.
PD JUL 13
PY 2016
VL 4
AR 73
DI 10.1186/s40478-016-0342-3
PG 11
WC Neurosciences
SC Neurosciences & Neurology
GA DQ9IU
UT WOS:000379524400001
PM 27411570
ER
PT J
AU Xu, BB
Luo, ZP
Wilson, AJ
Chen, K
Gao, WX
Yuan, GL
Chopra, HD
Chen, X
Willets, KA
Dauter, Z
Ren, SQ
AF Xu, Beibei
Luo, Zhipu
Wilson, Andrew J.
Chen, Ke
Gao, Wenxiu
Yuan, Guoliang
Chopra, Harsh Deep
Chen, Xing
Willets, Katherine A.
Dauter, Zbigniew
Ren, Shenqiang
TI Multifunctional Charge-Transfer Single Crystals through Supramolecular
Assembly
SO ADVANCED MATERIALS
LA English
DT Article
ID TETRACYANOQUINODIMETHANE TTF-TCNQ; TRANSFER COMPLEXES; TRANSFER
INTERFACES; ELECTRON-TRANSFER; TRANSFER SALT; MULTIFERROICITY;
FERROELECTRICITY; SEMICONDUCTOR; CONDUCTIVITY; MAGNETISM
AB Centimeter-sized segregated stacking TTF-C-60 single crystals are crystallized by a mass-transport approach combined with solvent-vapor evaporation for the first time. The intermolecular charge-transfer interaction in the long-range ordered superstructure enables the crystals to demonstrate external stimuli-controlled multifunctionalities and angle/electrical-potential-dependent luminescence.
C1 [Xu, Beibei; Chopra, Harsh Deep; Ren, Shenqiang] Temple Univ, Dept Mech Engn, Temple Mat Inst, Philadelphia, PA 19122 USA.
[Luo, Zhipu; Dauter, Zbigniew] Argonne Natl Lab, Synchrotron Radiat Res Sect, Macromol Crystallog Lab, Natl Canc Inst, 9700 S Cass Ave, Argonne, IL 60439 USA.
[Wilson, Andrew J.; Willets, Katherine A.] Temple Univ, Dept Chem, Philadelphia, PA 19122 USA.
[Chen, Ke] Temple Univ, Dept Phys, Temple Mat Inst, Philadelphia, PA 19122 USA.
[Gao, Wenxiu; Yuan, Guoliang] Nanjing Univ Sci Technol, Sch Mat Sci & Engn, Nanjing 210094, Jiangsu, Peoples R China.
[Chen, Xing] Argonne Natl Lab, Div Energy Syst, 9700 S Cass Ave, Argonne, IL 60439 USA.
RP Ren, SQ (reprint author), Temple Univ, Dept Mech Engn, Temple Mat Inst, Philadelphia, PA 19122 USA.
EM shenqiang.ren@temple.edu
OI Wilson, Andrew/0000-0003-3427-810X
FU Army Research Office, Young Investigator Program [W911NF-15-1-0610];
U.S. Department of Energy, Basic Energy Sciences Award
[DE-FG02-13ER46937]; Department of Energy (DOE), Office of Science,
Basic Energy Science (BES) [DE-SC0010307]; National Science Foundation
DMR , Condensed Matter Physics [1541236]; Temple University's OVPR's
Infrastructure Grant; U.S. Department of Energy, Office of Science,
Office of Basic Energy Sciences [W-31-109-Eng-38]
FX Work at Temple University (S.R.) was supported by the Army Research
Office, Young Investigator Program (W911NF-15-1-0610, material
design/self-assembly of carbon photovoltaics), and the U.S. Department
of Energy, Basic Energy Sciences Award No. DE-FG02-13ER46937 (organic
synthesis and physical property measurement). Work at Temple University
(A.J.W. and K.A.W.) was supported by the Department of Energy (DOE),
Office of Science, Basic Energy Science (BES) under Award No.
DE-SC0010307. H.D.C. gratefully acknowledges support from the National
Science Foundation DMR, Condensed Matter Physics under Grant No. 1541236
(previously Grant No. 1309712) and Temple University's OVPR's
Infrastructure Grant. Diffraction data were collected at the SER-CAT
beamline 22BM at the Advanced Photon Source, Argonne National
Laboratory. Use of the Advanced Photon Source was supported by the U.S.
Department of Energy, Office of Science, Office of Basic Energy
Sciences, under Contract No. W-31-109-Eng-38. The authors also thank
Temple Materials Institute and Dr. X. Xi for the AFM measurements.
NR 48
TC 0
Z9 0
U1 24
U2 61
PU WILEY-V C H VERLAG GMBH
PI WEINHEIM
PA POSTFACH 101161, 69451 WEINHEIM, GERMANY
SN 0935-9648
EI 1521-4095
J9 ADV MATER
JI Adv. Mater.
PD JUL 13
PY 2016
VL 28
IS 26
BP 5322
EP +
DI 10.1002/adma.201600383
PG 9
WC Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience &
Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied;
Physics, Condensed Matter
SC Chemistry; Science & Technology - Other Topics; Materials Science;
Physics
GA DR5JH
UT WOS:000379938800024
PM 27146726
ER
PT J
AU Auslander, N
Yizhak, K
Weinstock, A
Budhu, A
Tang, W
Wang, XW
Ambs, S
Ruppin, E
AF Auslander, Noam
Yizhak, Keren
Weinstock, Adam
Budhu, Anuradha
Tang, Wei
Wang, Xin Wei
Ambs, Stefan
Ruppin, Eytan
TI A joint analysis of transcriptomic and metabolomic data uncovers
enhanced enzyme-metabolite coupling in breast cancer
SO SCIENTIFIC REPORTS
LA English
DT Article
ID HEPATOCELLULAR-CARCINOMA; LIPID-METABOLISM; PROSTATE-CANCER; EXPRESSION;
INTEGRATION; TUMORS; CLASSIFICATION; DYSREGULATION; ACCUMULATION;
PROGRESSION
AB Disrupted regulation of cellular processes is considered one of the hallmarks of cancer. We analyze metabolomic and transcriptomic profiles jointly collected from breast cancer and hepatocellular carcinoma patients to explore the associations between the expression of metabolic enzymes and the levels of the metabolites participating in the reactions they catalyze. Surprisingly, both breast cancer and hepatocellular tumors exhibit an increase in their gene-metabolites associations compared to noncancerous adjacent tissues. Following, we build predictors of metabolite levels from the expression of the enzyme genes catalyzing them. Applying these predictors to a large cohort of breast cancer samples we find that depleted levels of key cancer-related metabolites including glucose, glycine, serine and acetate are significantly associated with improved patient survival. Thus, we show that the levels of a wide range of metabolites in breast cancer can be successfully predicted from the transcriptome, going beyond the limited set of those measured.
C1 [Auslander, Noam; Ruppin, Eytan] Univ Maryland, Ctr Bioinformat & Computat Biol, College Pk, MD 20742 USA.
[Auslander, Noam; Ruppin, Eytan] Univ Maryland, Dept Comp Sci, College Pk, MD 20742 USA.
[Yizhak, Keren; Weinstock, Adam; Ruppin, Eytan] Tel Aviv Univ, Blavatnik Sch Comp Sci, IL-69978 Tel Aviv, Israel.
[Budhu, Anuradha; Wang, Xin Wei] NCI, Liver Carcinogenesis Sect, Human Carcinogenesis Lab, Ctr Canc Res, Bethesda, MD 20892 USA.
[Tang, Wei; Ambs, Stefan] NCI, Mol Epidemiol Sect, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA.
[Ruppin, Eytan] Tel Aviv Univ, Sackler Sch Med, IL-69978 Tel Aviv, Israel.
RP Auslander, N; Ruppin, E (reprint author), Univ Maryland, Ctr Bioinformat & Computat Biol, College Pk, MD 20742 USA.; Auslander, N; Ruppin, E (reprint author), Univ Maryland, Dept Comp Sci, College Pk, MD 20742 USA.; Yizhak, K; Ruppin, E (reprint author), Tel Aviv Univ, Blavatnik Sch Comp Sci, IL-69978 Tel Aviv, Israel.; Ruppin, E (reprint author), Tel Aviv Univ, Sackler Sch Med, IL-69978 Tel Aviv, Israel.
EM noamaus@gmail.com; kerenyiz@post.tau.ac.il; eyruppin@gmail.com
RI Wang, Xin/B-6162-2009
NR 43
TC 0
Z9 0
U1 7
U2 8
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2045-2322
J9 SCI REP-UK
JI Sci Rep
PD JUL 13
PY 2016
VL 6
AR 29662
DI 10.1038/srep29662
PG 10
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR0EB
UT WOS:000379579700001
PM 27406679
ER
PT J
AU Ganapathi, KA
Jobanputra, V
Iwamoto, F
Jain, P
Chen, JL
Cascione, L
Nahum, O
Levy, B
Xie, Y
Khattar, P
Hoehn, D
Bertoni, F
Murty, VV
Pittaluga, S
Jaffe, ES
Alobeid, B
Mansukhani, MM
Bhagat, G
AF Ganapathi, Karthik A.
Jobanputra, Vaidehi
Iwamoto, Fabio
Jain, Preti
Chen, Jinli
Cascione, Luciano
Nahum, Odelia
Levy, Brynn
Xie, Yi
Khattar, Pallavi
Hoehn, Daniela
Bertoni, Francesco
Murty, Vundavalli V.
Pittaluga, Stefania
Jaffe, Elaine S.
Alobeid, Bachir
Mansukhani, Mahesh M.
Bhagat, Govind
TI The genetic landscape of dural marginal zone lymphomas
SO ONCOTARGET
LA English
DT Article
DE dural marginal zone lymphoma; genome; mutations; TNFAIP3; NOTCH2
ID B-CELL LYMPHOMA; NF-KAPPA-B; RECURRENT MUTATIONS; CODING GENOME; NOTCH2;
A20; ACTIVATION; PATHWAY; MALT
AB The dura is a rare site of involvement by marginal zone lymphoma (MZL) and the biology of dural MZL is not well understood. We performed genome-wide DNA copy number and targeted mutational analysis of 14 dural MZL to determine the genetic landscape of this entity. Monoallelic and biallelic inactivation of TNFAIP3 by mutation (n=5) or loss (n=1) was observed in 6/9 (67%) dural MZL exhibiting plasmacytic differentiation, including 3 IgG4+ cases. In contrast, activating NOTCH2 mutations were detected in 4/5 (80%) dural MZL displaying variable monocytoid morphology. Inactivating TBL1XR1 mutations were identified in all NOTCH2 mutated cases. Recurrent mutations in KLHL6 (n=2) and MLL2 (n=2) were also detected. Gains at 6p25.3 (n=2) and losses at 1p36.32 (n=3) were common chromosomal imbalances, with loss of heterozygosity (LOH) of these loci observed in a subset of cases. Translocations involving the IGH or MALT1 genes were not identified. Our results indicate genetic similarities between dural MZL and other MZL subtypes. However, recurrent and mutually exclusive genetic alterations of TNFAIP3 and NOTCH2 appear to be associated with distinct disease phenotypes in dural MZL.
C1 [Ganapathi, Karthik A.; Jobanputra, Vaidehi; Jain, Preti; Chen, Jinli; Nahum, Odelia; Levy, Brynn; Hoehn, Daniela; Murty, Vundavalli V.; Alobeid, Bachir; Mansukhani, Mahesh M.; Bhagat, Govind] Columbia Univ, Dept Pathol & Cell Biol, Med Ctr, New York, NY 10027 USA.
[Iwamoto, Fabio] Columbia Univ, Dept Neurol, Med Ctr, New York, NY USA.
[Cascione, Luciano; Bertoni, Francesco] Oncol Res Inst, Bellinzona, Switzerland.
[Cascione, Luciano; Bertoni, Francesco] Oncol Inst Southern Switzerland, Bellinzona, Switzerland.
[Xie, Yi; Pittaluga, Stefania; Jaffe, Elaine S.] NCI, Hematopathol Sect, Pathol Lab, Bethesda, MD 20892 USA.
[Khattar, Pallavi] New York Med Coll, Dept Pathol, Valhalla, NY 10595 USA.
RP Mansukhani, MM; Bhagat, G (reprint author), Columbia Univ, Dept Pathol & Cell Biol, Med Ctr, New York, NY 10027 USA.
EM mm322@cumc.columbia.edu; gb96@cumc.columbia.edu
OI Bhagat, Govind/0000-0001-6250-048X
NR 35
TC 0
Z9 0
U1 1
U2 1
PU IMPACT JOURNALS LLC
PI ALBANY
PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA
SN 1949-2553
J9 ONCOTARGET
JI Oncotarget
PD JUL 12
PY 2016
VL 7
IS 28
BP 43052
EP 43061
DI 10.18632/oncotarget.9678
PG 10
WC Oncology; Cell Biology
SC Oncology; Cell Biology
GA DY8QU
UT WOS:000385395700017
PM 27248180
ER
PT J
AU Mocsar, G
Volko, J
Ronnlund, D
Widengren, J
Nagy, P
Szollosi, J
Toth, K
Goldman, CK
Damjanovich, S
Waldmann, TA
Bodnar, A
Vamosi, G
AF Mocsar, Gabor
Volko, Julianna
Ronnlund, Daniel
Widengren, Jerker
Nagy, Peter
Szollosi, Janos
Toth, Katalin
Goldman, Carolyn K.
Damjanovich, Sandor
Waldmann, Thomas A.
Bodnar, Andrea
Vamosi, Gyorgy
TI MHC I Expression Regulates Co-clustering and Mobility of Interleukin-2
and-15 Receptors in T Cells
SO BIOPHYSICAL JOURNAL
LA English
DT Article
ID RESONANCE ENERGY-TRANSFER; HLA CLASS-I; FLUORESCENCE CORRELATION
SPECTROSCOPY; HUMAN LYMPHOBLASTOID-CELLS; PLASMA-MEMBRANE;
MONOCLONAL-ANTIBODY; IL-2 RECEPTOR; INSULIN-RECEPTORS; LYMPHOMA-CELLS;
LIPID RAFTS
AB MHC glycoproteins form supramolecular clusters with interleukin-2 and -15 receptors in lipid rafts of T cells. The role of highly expressed MHC I in maintaining these clusters is unknown. We knocked down MHC I in FT7.10 human T cells, and studied protein clustering at two hierarchic levels: molecular aggregations and mobility by Forster resonance energy transfer and fluorescence correlation spectroscopy; and segregation into larger domains or superclusters by superresolution stimulated emission depletion microscopy. Fluorescence correlation spectroscopy-based molecular brightness analysis revealed that the studied molecules diffused as tight aggregates of several proteins of a kind. Knockdown reduced the number of MHC I containing molecular aggregates and their average MHC I content, and decreased the heteroassociation of MHC I with IL-2R alpha/IL-15R alpha. The mobility of not only MHC I but also that of IL-2R alpha/IL-15R alpha increased, corroborating the general size decrease of tight aggregates. A multifaceted analysis of stimulated emission depletion images revealed that the diameter of MHC I superclusters diminished from 400-600 to 200-300 nm, whereas those of IL-2R alpha/IL-15R alpha hardly changed. MHC I and IL-2R alpha/IL-15R alpha colocalized with GM1 ganglioside-rich lipid rafts, but MHC I clusters retracted to smaller subsets of GM1-and IL-2R alpha/IL-15R alpha-rich areas upon knockdown. Our results prove that changes in expression level may significantly alter the organization and mobility of interacting membrane proteins.
C1 [Mocsar, Gabor; Volko, Julianna; Nagy, Peter; Szollosi, Janos; Damjanovich, Sandor; Bodnar, Andrea; Vamosi, Gyorgy] Univ Debrecen, Fac Med, Dept Biophys & Cell Biol, Debrecen, Hungary.
[Ronnlund, Daniel; Widengren, Jerker] Albanova Univ Ctr, Royal Inst Technol, Dept Appl Phys Expt Biomol Phys, Stockholm, Sweden.
[Szollosi, Janos] Hungarian Acad Sci, Cell Biol & Signaling Res Grp, Debrecen, Hungary.
[Szollosi, Janos] Univ Debrecen, Debrecen, Hungary.
[Toth, Katalin] German Canc Res Ctr, Biophys Macromol, Heidelberg, Germany.
[Goldman, Carolyn K.; Waldmann, Thomas A.] NCI, Lymphoid Malignancies Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
RP Vamosi, G (reprint author), Univ Debrecen, Fac Med, Dept Biophys & Cell Biol, Debrecen, Hungary.
EM vamosig@med.unideb.hu
RI Nagy, Peter/D-2188-2013;
OI Nagy, Peter/0000-0002-7466-805X; Widengren, Jerker/0000-0003-3200-0374
FU Hungarian Scientific Research Fund [K103965, K103906, NK101337];
Intramural Research Program of the National Cancer Institute, National
Institutes of Health; TAMOP grant [4.2.2.-08/1-2008-0019]; "MOLMEDREX''
FP7-REGPOT-1 grant [229920]; Tempus Public Foundation Hungary [73163];
Internal Research Program of the University of Debrecen grant
[RH/885/2013]; Jedlik Anyos Fellowship grant [A2-JADJ-13-0170]; New
Hungary Development Plan - European Social Fund
[TAMOP-4.2.2.A-11/1/KONV-2012-0023 VED-ELEM,
TAMOP-4.2.2.D-15/1/KONV-2015-0016]; European Regional Development Fund;
European Union; State of Hungary; German Academic Exchange Service;
European Social Fund [TAMOP-4.2.4.A/2-11/1-20120001]
FX This work was supported by Hungarian Scientific Research Fund grant No.
K103965 to G.V., grant No. K103906 to P.N., and grant No. NK101337 to
J.S.; the Intramural Research Program of the National Cancer Institute,
National Institutes of Health to T.A.W.; TAMOP grant No.
4.2.2.-08/1-2008-0019 to G.V.; "MOLMEDREX'' FP7-REGPOT-2008-1 grant No.
229920 to G.V.; German Academic Exchange Service and Tempus Public
Foundation Hungary grant No. 73163 to G.V. and K.T.; Internal Research
Program of the University of Debrecen grant No. RH/885/2013 to A.B.;
Jedlik Anyos Fellowship grant No. A2-JADJ-13-0170 to G.M.;
TAMOP-4.2.2.A-11/1/KONV-2012-0023 VED-ELEM and
TAMOP-4.2.2.D-15/1/KONV-2015-0016 grants implemented through the New
Hungary Development Plan cofinanced by the European Social Fund and the
European Regional Development Fund to G.V. and J.S.; and the European
Union and the State of Hungary, cofinanced by the European Social Fund
in the framework of TAMOP-4.2.4.A/2-11/1-20120001 National Excellence
Program to G.V.
NR 77
TC 1
Z9 1
U1 3
U2 6
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0006-3495
EI 1542-0086
J9 BIOPHYS J
JI Biophys. J.
PD JUL 12
PY 2016
VL 111
IS 1
BP 100
EP 112
DI 10.1016/j.bpj.2016.05.044
PG 13
WC Biophysics
SC Biophysics
GA DS1QN
UT WOS:000380371400013
PM 27410738
ER
PT J
AU Collins, FS
AF Collins, Francis S.
TI Seeking a Cure for One of the Rarest Diseases: Progeria
SO CIRCULATION
LA English
DT Editorial Material
DE Editorials; everolimus; lamin type A; lonafarnib; morpholinos; progeria;
rare diseases; translational medical research
ID HUTCHINSON-GILFORD-PROGERIA
C1 [Collins, Francis S.] NHGRI, Med Genom & Metab Genet Branch, NIH, Bethesda, MD 20892 USA.
RP Collins, FS (reprint author), NHGRI, Med Genom & Metab Genet Branch, NIH, Bethesda, MD 20892 USA.
EM francis.collins3@nih.gov
NR 19
TC 1
Z9 1
U1 9
U2 10
PU LIPPINCOTT WILLIAMS & WILKINS
PI PHILADELPHIA
PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA
SN 0009-7322
EI 1524-4539
J9 CIRCULATION
JI Circulation
PD JUL 12
PY 2016
VL 134
IS 2
BP 126
EP 129
DI 10.1161/CIRCULATIONAHA.116.022965
PG 4
WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease
SC Cardiovascular System & Cardiology
GA DR8EA
UT WOS:000380130000010
PM 27400897
ER
PT J
AU Amar, MJA
Kaler, M
Courville, AB
Shamburek, R
Sampson, M
Remaley, AT
AF Amar, Marcelo J. A.
Kaler, Maryann
Courville, Amber B.
Shamburek, Robert
Sampson, Maureen
Remaley, Alan T.
TI Randomized double blind clinical trial on the effect of oral
alpha-cyclodextrin on serum lipids
SO LIPIDS IN HEALTH AND DISEASE
LA English
DT Article
DE alpha-cyclodextrin; TC; Total cholesterol; TG; Triglycerides; LIRI;
Lipoprotein Insulin Resistance Index; LDL-C; Low density lipoprotein
cholesterol; HDL-C; High density lipoprotein cholesterol; BAS; Bile acid
sequestrants
ID DENSITY-LIPOPROTEIN PARTICLES; TO-RETENTION HYPOTHESIS; BILE-ACID
SEQUESTRANTS; INSULIN-RESISTANCE; DIETARY FIBER; CHOLESTEROL; DISEASE;
RATS; ATHEROGENESIS; TRIGLYCERIDE
AB Background: This single center, double-blinded, cross-over, placebo controlled clinical trial investigated the effect of oral a-cyclodextrin (alpha-CD), a soluble dietary fiber, on blood lipid and lipoprotein levels in healthy human subjects. alpha-CD, a cyclical polymer containing 6 glucose subunits, is currently sold as an over the counter food supplement and is also a common additive in many foods. alpha-CD forms a hydrophobic central cavity that binds lipids and has been shown in animal studies and in previous clinical trials to alter plasma lipid levels.
Methods: We screened for healthy subjects, males and females, between ages 18 to 75. Out of total 103 subjects interviewed, 75 subjects completed the study. Qualified individuals in each gender group were randomized into two groups in terms of which treatment arm they received first (placebo vs. alpha-CD, receiving 6 grams P.O. a day, for 12-14 weeks with a 7 day wash out between arms). The primary outcome variable, plasma total cholesterol, as well as other tests related to lipids and lipoprotein and glucose metabolism, were measured at baseline and at the end of each arm of the study.
Results: alpha-CD was well tolerated; no serious adverse events related to alpha-CD were observed. Approximately 8 % of the subjects on alpha-CD complained of minor gastrointestinal symptoms versus 3 % on placebo (p = 0.2). Small-LDL particle number decreased 10 % (p < 0.045) for subjects on alpha-CD versus placebo. Fasting plasma glucose (1.6 %, p < 0.05) and Insulin resistance index (11 %, p < 0.04) were also decreased when on alpha-CD versus placebo.
Conclusion: alpha-CD treatment appears to be safe and well tolerated in healthy individuals and showed a modest reduction in small LDL particles, and an improvement in glucose related parameters.
C1 [Amar, Marcelo J. A.; Kaler, Maryann; Courville, Amber B.; Shamburek, Robert; Sampson, Maureen; Remaley, Alan T.] NHLBI, Lipoprot Metab Sect, Cardiopulm Branch, NIH, Bldg 10,Room 8 N-228,10 Ctr Dr MSC 1666, Bethesda, MD 20892 USA.
RP Amar, MJA (reprint author), NHLBI, Lipoprot Metab Sect, Cardiopulm Branch, NIH, Bldg 10,Room 8 N-228,10 Ctr Dr MSC 1666, Bethesda, MD 20892 USA.
EM ma90x@nih.gov
FU Intramural Research Program of the National Institutes of Health
[National Heart, Lung, and Blood Institute]
FX This research was supported by Intramural Research Program of the
National Institutes of Health [National Heart, Lung, and Blood
Institute].
NR 34
TC 2
Z9 2
U1 1
U2 3
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1476-511X
J9 LIPIDS HEALTH DIS
JI Lipids Health Dis.
PD JUL 12
PY 2016
VL 15
AR 115
DI 10.1186/s12944-016-0284-6
PG 8
WC Biochemistry & Molecular Biology; Nutrition & Dietetics
SC Biochemistry & Molecular Biology; Nutrition & Dietetics
GA DR6FE
UT WOS:000379996800001
PM 27405337
ER
PT J
AU Watters, SA
Mlcochova, P
Maldarelli, F
Goonetilleke, N
Pillay, D
Gupta, RK
AF Watters, Sarah A.
Mlcochova, Petra
Maldarelli, Frank
Goonetilleke, Nilu
Pillay, Deenan
Gupta, Ravindra K.
TI Sequential CCR5-Tropic HIV-1 Reactivation from Distinct Cellular
Reservoirs following Perturbation of Elite Control
SO PLOS ONE
LA English
DT Article
ID ACTIVE ANTIRETROVIRAL THERAPY; T-CELLS; CORECEPTOR USAGE; PHYLOGENIES;
DISCONTINUATION; IDENTIFICATION; REPLICATION; MACROPHAGES; PREDICTION
AB Background
HIV Elite Controllers may reveal insights into virus persistence given they harbour small reservoir sizes, akin to HIV non-controllers treated early with combination antiretroviral therapy. Both groups of patients represent the most promising candidates for interventions aimed at sustained remission or 'cure'. Analytic treatment interruption (ATI) in the latter group leads to stochastic rebound of virus, though it is unclear whether loss of elite control is also associated with similar rebound characteristics.
Methods
We studied three discrete periods of virus rebound during myeloma related immune disruption over 2.5 years in an elite controller who previously underwent autologous stem cell transplantation (ASCT) in the absence of any antiretroviral therapy. Single genome sequencing of the V1-V4 region of env in PBMC and plasma was performed and phylogenies reconstructed. Average pairwise distance (APD) was calculated and non-parametric methods used to assess compartmentalisation. Coreceptor usage was predicted based on genotypic algorithms.
Results
122 single genome sequences were obtained (median 26 sequences per rebound). The initial rebounding plasma env sequences following ASCT represented two distinct lineages, and clustered with proviral DNA sequences isolated prior to ASCT. One of the lineages was monophyletic, possibly indicating reactivation from clonally expanded cells. The second rebound occurred 470 days after spontaneous control of the first rebound and was phylogenetically distinct from the first, confirmed by compartmentalisation analysis, with a different cellular origin rather than ongoing replication. By contrast, third rebound viruses clustered with second rebound viruses, with evidence for ongoing evolution that was associated with lymphopenia and myeloma progression. Following ASCT a shift in tropism from CXCR4-tropic viruses to a CCR5-tropic population was observed to persist through to the third rebound.
Conclusions
Our data highlight similarities in the viral reservoir between elite and non-controllers undergoing ATI following allogeneic transplantation. The lack of propagation of CXCR4 using viruses following transplantation warrants further study.
C1 [Watters, Sarah A.; Mlcochova, Petra; Pillay, Deenan; Gupta, Ravindra K.] UCL, Div Infect & Immun, London, England.
[Watters, Sarah A.; Maldarelli, Frank] NCI, HIV Dynam & Replicat Program, Frederick, MD USA.
[Goonetilleke, Nilu] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC USA.
[Pillay, Deenan] Africa Ctr Hlth & Populat Studies, Durban, Kwazulu Natal, South Africa.
RP Gupta, RK (reprint author), UCL, Div Infect & Immun, London, England.
EM Ravindra.gupta@ucl.ac.uk
FU Wellcome Trust [WT098251AIA]; National Institutes for Health Research
University College London Hospitals Biomedical Research Centre
FX This work was supported by Wellcome Trust (SAW) WT098251AIA; National
Institutes for Health Research University College London Hospitals
Biomedical Research Centre. The funders had no role in study design,
data collection and analysis, decision to publish, or preparation of the
manuscript.
NR 31
TC 0
Z9 0
U1 4
U2 4
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 12
PY 2016
VL 11
IS 7
AR e0158854
DI 10.1371/journal.pone.0158854
PG 12
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ9CO
UT WOS:000379508200017
PM 27403738
ER
PT J
AU Liu, SH
Liu, J
Ma, Q
Cao, L
Fattah, RJ
Yu, ZX
Bugge, TH
Finkel, T
Leppla, SH
AF Liu, Shihui
Liu, Jie
Ma, Qian
Cao, Liu
Fattah, Rasem J.
Yu, Zuxi
Bugge, Thomas H.
Finkel, Toren
Leppla, Stephen H.
TI Solid tumor therapy by selectively targeting stromal endothelial cells
SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
LA English
DT Article
DE anthrax toxin; tumor targeting; angiogenesis; CMG2; TEM8
ID ANTHRAX LETHAL FACTOR; CAPILLARY MORPHOGENESIS PROTEIN-2; CHRONIC
LYMPHOCYTIC-LEUKEMIA; PROTECTIVE ANTIGEN; TOXIN VARIANTS; KINASE-KINASE;
CANCER; ANGIOGENESIS; RECEPTOR; MATRIX
AB Engineered tumor-targeted anthrax lethal toxin proteins have been shown to strongly suppress growth of solid tumors in mice. These toxins work through the native toxin receptors tumor endothelium marker-8 and capillary morphogenesis protein-2 (CMG2), which, in other contexts, have been described as markers of tumor endothelium. We found that neither receptor is required for tumor growth. We further demonstrate that tumor cells, which are resistant to the toxin when grown in vitro, become highly sensitive when implanted in mice. Using a range of tissue-specific loss-of-function and gain-of-function genetic models, we determined that this in vivo toxin sensitivity requires CMG2 expression on host-derived tumor endothelial cells. Notably, engineered toxins were shown to suppress the proliferation of isolated tumor endothelial cells. Finally, we demonstrate that administering an immunosuppressive regimen allows animals to receive multiple toxin dosages and thereby produces a strong and durable antitumor effect. The ability to give repeated doses of toxins, coupled with the specific targeting of tumor endothelial cells, suggests that our strategy should be efficacious for a wide range of solid tumors.
C1 [Liu, Shihui; Ma, Qian; Fattah, Rasem J.; Leppla, Stephen H.] NIAID, Microbial Pathogenesis Sect, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Liu, Shihui; Bugge, Thomas H.] Natl Inst Dent & Craniofacial Res, Proteases & Tissue Remodeling Sect, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD 20892 USA.
[Liu, Jie; Cao, Liu; Finkel, Toren] NHLBI, Ctr Mol Med, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Yu, Zuxi] NHLBI, Pathol Core Facil, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Cao, Liu] China Med Univ, Minist Educ, Key Lab Med Cell Biol, Shenyang 110001, Peoples R China.
RP Liu, SH; Leppla, SH (reprint author), NIAID, Microbial Pathogenesis Sect, Parasit Dis Lab, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.; Liu, SH (reprint author), Natl Inst Dent & Craniofacial Res, Proteases & Tissue Remodeling Sect, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD 20892 USA.
EM shihui.liu@nih.gov; sleppla@niaid.nih.gov
FU Divisions of Intramural Research of the National Institute of Allergy
and Infectious Diseases; National Heart, Lung, and Blood Institute;
National Institute of Dental and Craniofacial Research, National
Institutes of Health
FX We thank Mahtab Moayeri for helpful discussions. This research was
supported with funds from the Divisions of Intramural Research of the
National Institute of Allergy and Infectious Diseases, the National
Heart, Lung, and Blood Institute, and the National Institute of Dental
and Craniofacial Research, National Institutes of Health.
NR 54
TC 1
Z9 1
U1 7
U2 7
PU NATL ACAD SCIENCES
PI WASHINGTON
PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
SN 0027-8424
J9 P NATL ACAD SCI USA
JI Proc. Natl. Acad. Sci. U. S. A.
PD JUL 12
PY 2016
VL 113
IS 28
BP E4079
EP E4087
DI 10.1073/pnas.1600982113
PG 9
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR1VX
UT WOS:000379694100014
PM 27357689
ER
PT J
AU Fauci, AS
AF Fauci, Anthony S.
TI An HIV Vaccine Mapping Uncharted Territory
SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
LA English
DT Editorial Material
ID INFECTION
C1 [Fauci, Anthony S.] NIAID, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
RP Fauci, AS (reprint author), NIAID, Immunoregulat Lab, Bldg 31,Room 7A-03,31 Ctr Dr,MSC 2520, Bethesda, MD 20892 USA.
EM afauci@niaid.nih.gov
NR 7
TC 1
Z9 1
U1 1
U2 2
PU AMER MEDICAL ASSOC
PI CHICAGO
PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA
SN 0098-7484
EI 1538-3598
J9 JAMA-J AM MED ASSOC
JI JAMA-J. Am. Med. Assoc.
PD JUL 12
PY 2016
VL 316
IS 2
BP 143
EP 144
DI 10.1001/jama.2016.7538
PG 2
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ9CW
UT WOS:000379509000006
PM 27404178
ER
PT J
AU Metsch, LR
Feaster, DJ
Gooden, L
Matheson, T
Stitzer, M
Das, M
Jain, MK
Rodriguez, AE
Armstrong, WS
Lucas, GM
Nijhawan, AE
Drainoni, ML
Herrera, P
Vergara-Rodriguez, P
Jacobson, JM
Mugavero, MJ
Sullivan, M
Daar, ES
McMahon, DK
Ferris, DC
Lindblad, R
VanVeldhuisen, P
Oden, N
Castellon, PC
Tross, S
Haynes, LF
Douaihy, A
Sorensen, JL
Metzger, DS
Mandler, RN
Colfax, GN
del Rio, C
AF Metsch, Lisa R.
Feaster, Daniel J.
Gooden, Lauren
Matheson, Tim
Stitzer, Maxine
Das, Moupali
Jain, Mamta K.
Rodriguez, Allan E.
Armstrong, Wendy S.
Lucas, Gregory M.
Nijhawan, Ank E.
Drainoni, Mari-Lynn
Herrera, Patricia
Vergara-Rodriguez, Pamela
Jacobson, Jeffrey M.
Mugavero, Michael J.
Sullivan, Meg
Daar, Eric S.
McMahon, Deborah K.
Ferris, David C.
Lindblad, Robert
VanVeldhuisen, Paul
Oden, Neal
Castellon, Pedro C.
Tross, Susan
Haynes, Louise F.
Douaihy, Antoine
Sorensen, James L.
Metzger, David S.
Mandler, Raul N.
Colfax, Grant N.
del Rio, Carlos
TI Effect of Patient Navigation With or Without Financial Incentives on
Viral Suppression Among Hospitalized Patients With HIV Infection and
Substance Use A Randomized Clinical Trial
SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
LA English
DT Article
ID ADDICTION SEVERITY INDEX; CRACK COCAINE USERS; DRUG-USERS;
CASE-MANAGEMENT; SCREENING-TEST; CARE; RETENTION; ADHERENCE; STATES;
PROGRESSION
AB IMPORTANCE Substance use is a major driver of the HIV epidemic and is associated with poor HIV care outcomes. Patient navigation (care coordination with case management) and the use of financial incentives for achieving predetermined outcomes are interventions increasingly promoted to engage patients in substance use disorders treatment and HIV care, but there is little evidence for their efficacy in improving HIV-1 viral suppression rates.
OBJECTIVE To assess the effect of a structured patient navigation intervention with or without financial incentives to improve HIV-1 viral suppression rates among patients with elevated HIV-1 viral loads and substance use recruited as hospital inpatients.
DESIGN, SETTING, AND PARTICIPANTS From July 2012 through January 2014, 801 patients with HIV infection and substance use from 11 hospitals across the United States were randomly assigned to receive patient navigation alone (n = 266), patient navigation plus financial incentives (n = 271), or treatment as usual (n = 264). HIV-1 plasma viral load was measured at baseline and at 6 and 12 months.
INTERVENTIONS Patient navigation included up to 11 sessions of care coordination with case management and motivational interviewing techniques over 6 months. Financial incentives (up to $1160) were provided for achieving targeted behaviors aimed at reducing substance use, increasing engagement in HIV care, and improving HIV outcomes. Treatment as usual was the standard practice at each hospital for linking hospitalized patients to outpatient HIV care and substance use disorders treatment.
MAIN OUTCOMES AND MEASURES The primary outcome was HIV viral suppression (<= 200 copies/mL) relative to viral nonsuppression or death at the 12-month follow-up.
RESULTS Of 801 patients randomized, 261 (32.6%) were women (mean [SD] age, 44.6 years [10.0 years]). There were no differences in rates of HIV viral suppression versus nonsuppression or death among the 3 groups at 12 months. Eighty-five of 249 patients (34.1%) in the usual-treatment group experienced treatment success compared with 89 of 249 patients (35.7%) in the navigation-only group for a treatment difference of 1.6% (95% CI, -6.8% to 10.0%; P = .80) and compared with 98 of 254 patients (38.6%) in the navigation-plus-incentives group for a treatment difference of 4.5%(95% CI -4.0% to 12.8%; P = .68). The treatment difference between the navigation-only and the navigation-plus-incentives group was -2.8%(95% CI, -11.3% to 5.6%; P = .68).
CONCLUSIONS AND RELEVANCE Among hospitalized patients with HIV infection and substance use, patient navigation with or without financial incentives did not have a beneficial effect on HIV viral suppression relative to nonsuppression or death at 12 months vs treatment as usual. These findings do not support these interventions in this setting.
C1 [Metsch, Lisa R.; Gooden, Lauren; Castellon, Pedro C.] Columbia Univ, Dept Sociomed Sci, Mailman Sch Publ Hlth, 722 W168th St,Room 918, New York, NY 10032 USA.
[Feaster, Daniel J.] Univ Miami, Miller Sch Med, Dept Publ Hlth Sci, Miami, FL 33136 USA.
[Matheson, Tim; Das, Moupali; Colfax, Grant N.] San Francisco Dept Publ Hlth, San Francisco, CA USA.
[Stitzer, Maxine] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA.
[Das, Moupali] San Francisco Gen Hosp, San Francisco, CA 94110 USA.
[Das, Moupali] Univ Calif San Francisco, San Francisco, CA 94143 USA.
[Jain, Mamta K.; Nijhawan, Ank E.] Univ Texas Southwestern Med Ctr Dallas, Div Infect Dis, Dept Internal Med, Dallas, TX USA.
[Jain, Mamta K.] Parkland Hlth & Hosp Syst, Dallas, TX USA.
[Rodriguez, Allan E.] Univ Miami, Miller Sch Med, Div Infect Dis, Dept Med, Miami, FL 33136 USA.
[Armstrong, Wendy S.; del Rio, Carlos] Emory Univ, Sch Med, Dept Med, Div Infect Dis, Atlanta, GA USA.
[Lucas, Gregory M.] Johns Hopkins Univ, Sch Med, Baltimore, MD USA.
[Drainoni, Mari-Lynn] Boston Univ, Sch Publ Hlth, Dept Hlth Law Policy & Management, Boston, MA USA.
[Drainoni, Mari-Lynn; Sullivan, Meg] Boston Univ, Sch Med, Dept Med, Infect Dis Sect, Boston, MA 02118 USA.
[Herrera, Patricia; Vergara-Rodriguez, Pamela] John H Stroger Jr Hosp Cook Cty, Ruth M Rothstein CORE Ctr, Chicago, IL USA.
[Jacobson, Jeffrey M.] Drexel Univ, Div Infect Dis, Coll Med, Philadelphia, PA 19104 USA.
[Jacobson, Jeffrey M.] Temple Univ, Lewis Katz Sch Med, Dept Med, Philadelphia, PA 19122 USA.
[Jacobson, Jeffrey M.] Temple Univ, Lewis Katz Sch Med, Dept Neurosci, Philadelphia, PA 19122 USA.
[Jacobson, Jeffrey M.] Temple Univ, Lewis Katz Sch Med, Inst Translat AIDS Res, Philadelphia, PA 19122 USA.
[Mugavero, Michael J.] Univ Alabama Birmingham, Dept Med, Div Infect Dis, Birmingham, AL 35294 USA.
[Daar, Eric S.] Univ Calif Los Angeles, Med Ctr, Los Angeles Biomed Res Inst Harbor, Torrance, CA 90509 USA.
[McMahon, Deborah K.] Univ Pittsburgh, Pittsburgh, PA USA.
[Ferris, David C.] Mt Sinai St Lukes & Mt Sinai West Hosp, New York, NY USA.
[Ferris, David C.] Icahn Sch Med Mt Sinai, New York, NY 10029 USA.
[Lindblad, Robert; VanVeldhuisen, Paul; Oden, Neal] Emmes Corp, Rockville, MD USA.
[Tross, Susan] Columbia Univ, HIV Ctr Clin & Behav Studies, Div Gender Sexual & Hlth, Dept Psychiat, New York, NY USA.
[Tross, Susan] Columbia Univ, Greater New York Node, Natl Drug Abuse Treatment Clin Trials Network, Substance Use Res Ctr,Dept Psychiat, New York, NY USA.
[Haynes, Louise F.] Med Univ South Carolina, Div Addict Sci, Dept Psychiat & Behav Sci, Charleston, SC 29425 USA.
[Douaihy, Antoine] Univ Pittsburgh, Sch Med, Pittsburgh, PA USA.
[Sorensen, James L.] Univ Calif San Francisco, Dept Psychiat, Western States Node, Natl Drug Abuse Treatment Clin Trials Network, San Francisco, CA USA.
[Metzger, David S.] Univ Penn, Dept Psychiat, Perelman Sch Med, Philadelphia, PA 19104 USA.
[Metzger, David S.] Treatment Res Inst, Philadelphia, PA USA.
[Mandler, Raul N.] NIDA, Ctr Clin Trials Network, NIH, Bethesda, MD 20892 USA.
[del Rio, Carlos] Emory Univ, Hubert Dept Global Hlth, Rollins Sch Publ Hlth, Atlanta, GA 30322 USA.
RP Metsch, LR (reprint author), Columbia Univ, Dept Sociomed Sci, Mailman Sch Publ Hlth, 722 W168th St,Room 918, New York, NY 10032 USA.
EM lm2892@columbia.edu
RI del Rio, Carlos/B-3763-2012
OI del Rio, Carlos/0000-0002-0153-3517
FU National Institute on Drug Abuse [U10DA013720, UG1DA013720, U10DA013035,
UG1DA013035, U10DA013034, UG1DA013034]; University of Miami Center for
AIDS Research (CFAR) [P30AI073961]; Emory University CFAR [P30AI050409];
Atlanta Clinical and Translational Science Institute [UL1TR000454]; HIV
Center for Clinical and Behavioral Studies at the New York State
Psychiatric Institute/Columbia University Medical Center [P30MH043520];
The National Institute on Drug Abuse [U10DA013727, UG1DA013727,
U10DA020024, UG1DA020024, U10DA013732, UG1DA013732, U10DA015831,
UG1DA015831, U10DA015815, UG1DA015815, U10DA020036, U10DA013043,
U10DA013045, HHSN271200900034C/N01DA92217,
HHSN271201400028C/N01DA142237, HHSN271201000024C/N01DA102221]
FX Funding for this study and analysis was provided for the study's
principal investigators by the National Institute on Drug Abuse under
the following awards: U10DA013720 and UG1DA013720 (Drs Jose Szapocznik
and Lisa R. Metsch); U10DA013035 and UG1DA013035 (Drs John Rotrosen and
Edward V. Nunes, Jr); U10DA013034 and UG1DA013034 (Drs Maxine Stitzer
and Robert Schwartz); U10DA013727 and UG1DA013727 (Drs. Kathleen T.
Brady and Matthew Carpenter); U10DA020024 and UG1DA020024 (Dr Madhukar
H. Trivedi); U10DA013732 and UG1DA013732 (Dr Theresa Winhusen);
U10DA015831 and UG1DA015831 (Drs. Roger D. Weiss and Kathleen Carroll);
U10DA015815 and UG1DA015815 (Drs James L. Sorensen and Dennis McCarty);
U10DA020036 (Dr Dennis Daley); U10DA013043 (Dr George Woody);
U10DA013045 (Dr Walter Ling); HHSN271200900034C/N01DA92217 and
HHSN271201400028C/N01DA142237 (Dr Paul Van Veldhuisen); and
HHSN271201000024C/N01DA102221 (Dr Robert Lindblad). Support from the
University of Miami Center for AIDS Research (CFAR) (P30AI073961; Dr
Savita Pahwa), the Emory University CFAR (P30AI050409; Drs Carlos del
Rio, James W. Curran, and Eric Hunter), the Atlanta Clinical and
Translational Science Institute (UL1TR000454; Dr David Stephens), and
the HIV Center for Clinical and Behavioral Studies at the New York State
Psychiatric Institute/Columbia University Medical Center (P30MH043520;
Dr Robert Remien) is also acknowledged.
NR 36
TC 7
Z9 7
U1 3
U2 6
PU AMER MEDICAL ASSOC
PI CHICAGO
PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA
SN 0098-7484
EI 1538-3598
J9 JAMA-J AM MED ASSOC
JI JAMA-J. Am. Med. Assoc.
PD JUL 12
PY 2016
VL 316
IS 2
BP 156
EP 170
DI 10.1001/jama.2016.8914
PG 15
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ9CW
UT WOS:000379509000012
PM 27404184
ER
PT J
AU Kong, XR
Kigozi, G
Ssekasanvu, J
Nalugoda, F
Nakigozi, G
Ndyanabo, A
Lutalo, T
Reynolds, SJ
Ssekubugu, R
Kagaayi, J
Bugos, E
Chang, LW
Nanlesta, P
Mary, G
Berman, A
Quinn, TC
Serwadda, D
Wawer, MJ
Gray, RH
AF Kong, Xiangrong
Kigozi, Godfrey
Ssekasanvu, Joseph
Nalugoda, Fred
Nakigozi, Gertrude
Ndyanabo, Anthony
Lutalo, Tom
Reynolds, Steven J.
Ssekubugu, Robert
Kagaayi, Joseph
Bugos, Eva
Chang, Larry W.
Nanlesta, Pilgrim
Mary, Grabowski
Berman, Amanda
Quinn, Thomas C.
Serwadda, David
Wawer, Maria J.
Gray, Ronald H.
TI Association of Medical Male Circumcision and Antiretroviral Therapy
Scale-up With Community HIV Incidence in Rakai, Uganda
SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
LA English
DT Article
ID PREVENTION; TRIAL; MEN; ACQUISITION; INFECTIONS; COVERAGE; RISK
AB IMPORTANCE Medical male circumcision (MMC) and antiretroviral therapy (ART) are proven HIV prevention interventions, but there are limited data on the population-level effect of scale-up of these interventions in sub-Saharan Africa. Such evaluation is important for planning and resource allocation.
OBJECTIVE To examine whether increasing community MMC and ART coverage was associated with reduced community HIV incidence in Rakai District, Uganda.
DESIGN, SETTING, AND PARTICIPANTS Using person-level data from population-based surveys conducted from 1999 through 2013 in 45 rural Rakai communities, community-level ART and MMC coverage, sociodemographics, sexual behaviors, and HIV prevalence and incidence were estimated in 3 periods: prior to the availability of ART and MMC (1999-2004), during early availability of ART and MMC (2004-2007), and during mature program scale-up (2007-2013).
EXPOSURES Community MMC coverage in males and ART coverage in HIV-positive persons of the opposite sex based on self-reported MMC status and ART use.
MAIN OUTCOMES AND MEASURES Adjusted incidence rate ratios (IRRs) for sex-specific community HIV incidence estimated using multivariable Poisson regression with generalized estimating equations.
RESULTS From 1999 through 2013, 44 688 persons participated in 1 or more surveys (mean age at the first survey, 24.6 years [range, 15-49]; female, 56.5%; mean survey participation rate, 92.6%[95% CI, 92.4%-92.7%]). Median community MMC coverage increased from 19% to 39%, and median community ART coverage rose from 0% to 21% in males and from 0% to 26% in females. Median community HIV incidence declined from 1.25 to 0.84 per 100 person-years in males, and from 1.25 to 0.99 per 100 person-years in females. Among males, each 10% increase in community MMC coverage was associated with an adjusted IRR of 0.87 (95% CI, 0.82-0.93). Comparing communities with MMC coverage more than 40% (mean male community incidence, 1.03 per 100 person-years) with communities with coverage of 10% or less (mean male incidence, 1.69 per 100 person-years), the adjusted IRR was 0.61 (95% CI, 0.43-0.88). For each 10% increase in female self-reported ART coverage, there was no significant reduction in male HIV incidence (adjusted IRR, 0.95 [95% CI, 0.81-1.13]). Comparing communities with female ART coverage more than 20%(mean male incidence, 0.87 per 100 person-years) to communities with female ART coverage of 20% or less (mean male incidence, 1.17 per 100 person-years), the adjusted IRR was 0.77 (95% CI, 0.61-0.98). Neither MMC nor male ART coverage was associated with lower female community HIV incidence.
CONCLUSIONS AND RELEVANCE In Rakai, Uganda, increasing community MMC and female ART coverage was associated with lower community HIV incidence in males. If similar associations are found elsewhere, this would support further scale-up of MMC and ART for HIV prevention in sub-Saharan Africa.
C1 [Kong, Xiangrong; Ssekasanvu, Joseph; Bugos, Eva; Chang, Larry W.; Mary, Grabowski; Wawer, Maria J.; Gray, Ronald H.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA.
[Kong, Xiangrong] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Biostat, Baltimore, MD USA.
[Kigozi, Godfrey; Ssekasanvu, Joseph; Nalugoda, Fred; Nakigozi, Gertrude; Ndyanabo, Anthony; Lutalo, Tom; Ssekubugu, Robert; Kagaayi, Joseph; Serwadda, David; Wawer, Maria J.; Gray, Ronald H.] Rakai Hlth Sci Program, Entebbe, Uganda.
[Reynolds, Steven J.; Chang, Larry W.; Quinn, Thomas C.] Johns Hopkins Sch Med, Baltimore, MD USA.
[Reynolds, Steven J.; Quinn, Thomas C.] NIAID, Div Intramural Res, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Nanlesta, Pilgrim] Populat Council, Washington, DC USA.
[Berman, Amanda] Johns Hopkins, Ctr Commun Programs, Baltimore, MD USA.
[Serwadda, David] Makerere Univ, Sch Publ Hlth, Kampala, Uganda.
RP Kong, XR (reprint author), Johns Hopkins Bloomberg Sch Publ Hlth, 627 N Washington St, Baltimore, MD 21205 USA.
EM xkong4@jhu.edu
FU National Institutes of Health (NIH) [K25AI114461, U01AI100031,
R01HD050180, U01AI075115, R01 HD070769]; Division of Intramural Research
at the National Institute of Allergy and Infectious Diseases; Bill and
Melinda Gates Foundation [081113, 22006.03]; Johns Hopkins Center for
AIDS Research [JHU CFAR P30AI094189]
FX This study was supported by grants from the National Institutes of
Health (NIH, K25AI114461, U01AI100031, R01HD050180, U01AI075115, R01
HD070769), the Division of Intramural Research at the National Institute
of Allergy and Infectious Diseases, the Bill and Melinda Gates
Foundation (#081113, 22006.03), and the Johns Hopkins Center for AIDS
Research (JHU CFAR P30AI094189).
NR 24
TC 2
Z9 2
U1 3
U2 3
PU AMER MEDICAL ASSOC
PI CHICAGO
PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA
SN 0098-7484
EI 1538-3598
J9 JAMA-J AM MED ASSOC
JI JAMA-J. Am. Med. Assoc.
PD JUL 12
PY 2016
VL 316
IS 2
BP 182
EP 190
DI 10.1001/jama.2016.7292
PG 9
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ9CW
UT WOS:000379509000014
PM 27404186
ER
PT J
AU Chen, Y
Singh, S
Matsumoto, A
Manna, SK
Abdelmegeed, MA
Golla, S
Murphy, RC
Dong, HB
Song, BJ
Gonzalez, FJ
Thompson, DC
Vasiliou, V
AF Chen, Ying
Singh, Surendra
Matsumoto, Akiko
Manna, Soumen K.
Abdelmegeed, Mohamed A.
Golla, Srujana
Murphy, Robert C.
Dong, Hongbin
Song, Byoung-Joon
Gonzalez, Frank J.
Thompson, David C.
Vasiliou, Vasilis
TI Chronic Glutathione Depletion Confers Protection against Alcohol-induced
Steatosis: Implication for Redox Activation of AMP-activated Protein
Kinase Pathway
SO SCIENTIFIC REPORTS
LA English
DT Article
ID INDUCED LIVER-INJURY; POLYUNSATURATED FATTY-ACIDS; OXIDATIVE STRESS;
DEFICIENT MICE; GENE-EXPRESSION; KNOCKOUT MICE; ALDEHYDE DEHYDROGENASES;
MOLECULAR-MECHANISMS; SUPEROXIDE-DISMUTASE; METABOLIZING ENZYME
AB The pathogenesis of alcoholic liver disease (ALD) is not well established. However, oxidative stress and associated decreases in levels of glutathione (GSH) are known to play a central role in ALD. The present study examines the effect of GSH deficiency on alcohol-induced liver steatosis in Gclm knockout (KO) mice that constitutively have approximate to 15% normal hepatic levels of GSH. Following chronic (6 week) feeding with an ethanol-containing liquid diet, the Gclm KO mice were unexpectedly found to be protected against steatosis despite showing increased oxidative stress (as reflected in elevated levels of CYP2E1 and protein carbonyls). Gclm KO mice also exhibit constitutive activation of liver AMP-activated protein kinase (AMPK) pathway and nuclear factor-erythroid 2-related factor 2 target genes, and show enhanced ethanol clearance, altered hepatic lipid profiles in favor of increased levels of polyunsaturated fatty acids and concordant changes in expression of genes associated with lipogenesis and fatty acid oxidation. In summary, our data implicate a novel mechanism protecting against liver steatosis via an oxidative stress adaptive response that activates the AMPK pathway. We propose redox activation of the AMPK may represent a new therapeutic strategy for preventing ALD.
C1 [Chen, Ying; Singh, Surendra; Dong, Hongbin; Vasiliou, Vasilis] Yale Univ, Dept Environm Hlth Sci, New Haven, CT 06520 USA.
[Matsumoto, Akiko] Saga Univ, Sch Med, Dept Social Med, Saga 8498501, Japan.
[Manna, Soumen K.; Golla, Srujana; Gonzalez, Frank J.] NCI, Lab Metab, Ctr Canc Res, Bethesda, MD 20852 USA.
[Abdelmegeed, Mohamed A.; Song, Byoung-Joon] NIAAA, Lab Membrane Biochem & Biophys, NIH, Bethesda, MD 20892 USA.
[Murphy, Robert C.] Univ Colorado AMC, Dept Pharmacol, Aurora, CO 80045 USA.
[Thompson, David C.] Univ Colorado AMC, Dept Clin Pharm, Aurora, CO 80045 USA.
RP Vasiliou, V (reprint author), Yale Univ, Dept Environm Hlth Sci, New Haven, CT 06520 USA.
EM vasilis.vasiliou@yale.edu
FU NIH [AA022057, AA021724]; NIAAA [T32 AA007464]
FX This work is supported in part by NIH grants AA022057 (VV), AA021724
(VV). HD was supported by the NIAAA T32 AA007464.
NR 63
TC 1
Z9 1
U1 1
U2 9
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2045-2322
J9 SCI REP-UK
JI Sci Rep
PD JUL 12
PY 2016
VL 6
AR 29743
DI 10.1038/srep29743
PG 13
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ7MW
UT WOS:000379392600001
PM 27403993
ER
PT J
AU Steemers, B
Vicente-Grabovetsky, A
Barry, C
Smulders, P
Schroder, TN
Burgess, N
Doeller, CF
AF Steemers, Ben
Vicente-Grabovetsky, Alejandro
Barry, Caswell
Smulders, Peter
Schroder, Tobias Navarro
Burgess, Neil
Doeller, Christian F.
TI Hippocampal Attractor Dynamics Predict Memory-Based Decision Making
SO CURRENT BIOLOGY
LA English
DT Article
ID MEDIAL TEMPORAL-LOBE; COMPLEMENTARY-LEARNING-SYSTEMS; VOXEL
PATTERN-ANALYSIS; SPATIAL NAVIGATION; RECOGNITION MEMORY; REAL-WORLD;
REPRESENTATIONS; NETWORKS; CONTEXT; INTEGRATION
AB Memories are thought to be retrieved by attractor dynamics if a given input is sufficiently similar to a stored attractor state [1-5]. The hippocampus, a region crucial for spatial navigation [6-12] and episodic memory [13-18], has been associated with attractorbased computations [5, 9], receiving support from the way rodent place cells ``remap'' nonlinearly between spatial representations [19-22]. In humans, nonlinear response patterns have been reported in perceptual categorization tasks [23-25]; however, it remains elusive whether human memory retrieval is driven by attractor dynamics and what neural mechanisms might underpin them. To test this, we used a virtual reality [7, 11, 26-28] task where participants learned object-location associations within two distinct virtual reality environments. Participants were subsequently exposed to four novel intermediate environments, generated by linearly morphing the background landscapes of the familiar environments, while tracking fMRI activity. We show that linear changes in environmental context cause linear changes in activity patterns in sensory cortex but cause dynamic, nonlinear changes in both hippocampal activity pattern and remembered locations. Furthermore, the sigmoidal response in the hippocampus scaled with the strength of the sigmoidal pattern in spatial memory. These results indicate that mnemonic decisions in an ambiguous novel context relate to putative attractor dynamics in the hippocampus, which support the dynamic remapping of memories.
C1 [Steemers, Ben; Vicente-Grabovetsky, Alejandro; Smulders, Peter; Schroder, Tobias Navarro; Doeller, Christian F.] Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, NL-6525 EN Nijmegen, Netherlands.
[Steemers, Ben] NIMH, Neuropsychol Lab, NIH, Bldg 9, Bethesda, MD 20892 USA.
[Barry, Caswell] UCL, Res Dept Cell & Dev Biol, Gower St, London WC1E 6BT, England.
[Burgess, Neil] UCL Inst Cognit Neurosci, 17 Queen Sq, London WC1N 3AZ, England.
[Burgess, Neil] UCL Inst Neurol, Queen Sq, London WC1 3BG, England.
RP Steemers, B; Doeller, CF (reprint author), Radboud Univ Nijmegen, Donders Inst Brain Cognit & Behav, NL-6525 EN Nijmegen, Netherlands.; Steemers, B (reprint author), NIMH, Neuropsychol Lab, NIH, Bldg 9, Bethesda, MD 20892 USA.
EM ben.steemers@nih.gov; christian.doeller@donders.ru.nl
FU European Research Council [ERC-StG 261177]; Netherlands Organization for
Scientific Research [NWO-Vidi 452-12-009]; Wellcome Trust; Medical
Research Council, UK; Royal Society, UK [101208/z/13/z]
FX This work was supported by European Research Council (ERC-StG 261177)
and Netherlands Organization for Scientific Research (NWO-Vidi
452-12-009) fellowships awarded to C.F.D. N.B. is supported by the
Wellcome Trust and the Medical Research Council, UK. C.B. is funded by
the Wellcome Trust and the Royal Society, UK (101208/z/13/z). The
authors would like to thank S. Auger and O. Vikbladh for earlier
behavioral pilot work; S. Bosch for help with data acquisition; and A.
Backus and J. Bellmund for useful discussions.
NR 45
TC 1
Z9 1
U1 3
U2 3
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0960-9822
EI 1879-0445
J9 CURR BIOL
JI Curr. Biol.
PD JUL 11
PY 2016
VL 26
IS 13
BP 1750
EP 1757
DI 10.1016/j.cub.2016.04.063
PG 8
WC Biochemistry & Molecular Biology; Cell Biology
SC Biochemistry & Molecular Biology; Cell Biology
GA DY0PU
UT WOS:000384799500042
PM 27345167
ER
PT J
AU Lu, R
Wang, P
Parton, T
Zhou, Y
Chrysovergis, K
Rockowitz, S
Chen, WY
Abdel-Wahab, O
Wade, PA
Zheng, DY
Wang, GG
AF Lu, Rui
Wang, Ping
Parton, Trevor
Zhou, Yang
Chrysovergis, Kaliopi
Rockowitz, Shira
Chen, Wei-Yi
Abdel-Wahab, Omar
Wade, Paul A.
Zheng, Deyou
Wang, Gang Greg
TI Epigenetic Perturbations by Arg882-Mutated DNMT3A Potentiate Aberrant
Stem Cell Gene-Expression Program and Acute Leukemia Development
SO CANCER CELL
LA English
DT Article
ID ACUTE MYELOID-LEUKEMIA; MLL-REARRANGED LEUKEMIA; HEMATOPOIETIC STEM; DNA
METHYLATION; CLONAL HEMATOPOIESIS; H3K79 METHYLATION; HOX GENES;
MUTATIONS; LEUKEMOGENESIS; TRANSFORMATION
AB DNA methyltransferase 3A(DNMT3A) is frequently mutated in hematological cancers; however, the underlying oncogenic mechanism remains elusive. Here, we report that the DNMT3A mutational hotspot at Arg882 (DNMT3A(R882H)) cooperates with NRAS mutation to transform hematopoietic stem/progenitor cells and induce acute leukemia development. Mechanistically, DNMT3A(R882H) directly binds to and potentiates transactivation of stemness genes critical for leukemogenicity including Meis1, Mn1, and Hoxa gene cluster. DNMT3A(R882H) induces focal epigenetic alterations, including CpG hypomethylation and concurrent gain of active histone modifications, at cis-regulatory elements such as enhancers to facilitate gene transcription. CRISPR/Cas9-mediated ablation of a putative Meis1 enhancer carrying DNMT3A(R882H)-induced DNA hypomethylation impairs Meis1 expression. Importantly, DNMT3A(R882H)-induced gene-expression programs can be repressed through Dot1l inhibition, providing an attractive therapeutic strategy for DNMT3A-mutated leukemias.
C1 [Lu, Rui; Parton, Trevor; Wang, Gang Greg] Univ N Carolina, Sch Med, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA.
[Lu, Rui; Wang, Gang Greg] Univ N Carolina, Sch Med, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA.
[Wang, Ping; Zheng, Deyou] Albert Einstein Coll Med, Dept Neurol, Bronx, NY 10461 USA.
[Zhou, Yang] Univ N Carolina, McAllister Heart Inst, Dept Pathol & Lab Med, Sch Med, Chapel Hill, NC 27599 USA.
[Chrysovergis, Kaliopi; Wade, Paul A.] NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA.
[Rockowitz, Shira; Zheng, Deyou] Albert Einstein Coll Med, Dept Genet, Bronx, NY 10461 USA.
[Rockowitz, Shira; Zheng, Deyou] Albert Einstein Coll Med, Dept Neurosci, Bronx, NY 10461 USA.
[Chen, Wei-Yi] Natl Yang Ming Univ, Inst Biochem & Mol Biol, Taipei 11221, Taiwan.
[Abdel-Wahab, Omar] Mem Sloan Kettering Canc Ctr, Human Oncol & Pathogenesis Program, New York, NY 10065 USA.
[Wang, Gang Greg] Univ North Carolina Chapel Hill, Curriculum Genet & Mol Biol, Chapel Hill, NC 27599 USA.
RP Wang, GG (reprint author), Univ N Carolina, Sch Med, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA.; Wang, GG (reprint author), Univ N Carolina, Sch Med, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA.; Zheng, DY (reprint author), Albert Einstein Coll Med, Dept Neurol, Bronx, NY 10461 USA.; Zheng, DY (reprint author), Albert Einstein Coll Med, Dept Genet, Bronx, NY 10461 USA.; Zheng, DY (reprint author), Albert Einstein Coll Med, Dept Neurosci, Bronx, NY 10461 USA.; Wang, GG (reprint author), Univ North Carolina Chapel Hill, Curriculum Genet & Mol Biol, Chapel Hill, NC 27599 USA.
EM deyou.zheng@einstein.yu.edu; greg_wang@med.unc.edu
OI Wang, G Greg/0000-0002-7210-9940; Lu, Rui/0000-0003-1593-2612
FU NCI [K99/R00, CA151683]; DoD [CA130247]; Gabrielle's Angel Foundation;
North Carolina Biotech Center Institutional Support Grant
[2012-IDG-1006]; UNC Cancer Center Core Support Grant [P30 CA016086]
FX We graciously thank Drs. Y. Xiong and K. Humphries for providing
constructs, M. Kamps and M. Minden for leukemia lines, M. Torres for
Meis1 antibody, D. Bauer and F. Zhang for CRISPR/Cas9 systems, M. Rehli
for a CpG-free reporter, and J. Bear for an shRNA vector used in this
study. Thanks to Drs. D. Allison and L. Cai and other members of the
Wang laboratory for helpful discussion and technical support. We thank
UNC's Genomics Core, Animal Studies Core, Flow Core, and HTSF core for
their support of this work. This work is supported by NCI K99/R00 grant
CA151683 to G.G.W., a DoD grant CA130247 to G.G.W., and grants of
Gabrielle's Angel Foundation to O.A. and G.G.W. G.G.W. is an American
Society of Hematology Scholar and a Kimmel Scholar. R.L. is a Lymphoma
Research Foundation Postdoctorate Fellow. UNC Cores including the flow
cytometry facility are supported in part by the North Carolina Biotech
Center Institutional Support Grant 2012-IDG-1006 and UNC Cancer Center
Core Support Grant P30 CA016086.
NR 45
TC 5
Z9 5
U1 3
U2 10
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 1535-6108
EI 1878-3686
J9 CANCER CELL
JI Cancer Cell
PD JUL 11
PY 2016
VL 30
IS 1
BP 92
EP 107
DI 10.1016/j.ccell.2016.05.008
PG 16
WC Oncology; Cell Biology
SC Oncology; Cell Biology
GA DR7JY
UT WOS:000380077100014
PM 27344947
ER
PT J
AU Di Biase, S
Lee, C
Brandhorst, S
Manes, B
Buono, R
Cheng, CW
Cacciottolo, M
Martin-Montalvo, A
de Cabo, R
Wei, M
Morgan, TE
Longo, VD
AF Di Biase, Stefano
Lee, Changhan
Brandhorst, Sebastian
Manes, Brianna
Buono, Roberta
Cheng, Chia-Wei
Cacciottolo, Mafalda
Martin-Montalvo, Alejandro
de Cabo, Rafael
Wei, Min
Morgan, Todd E.
Longo, Valter D.
TI Fasting-Mimicking Diet Reduces HO-1 to Promote T Cell-Mediated Tumor
Cytotoxicity
SO CANCER CELL
LA English
DT Article
ID HEME OXYGENASE-1; CARBON-MONOXIDE; BREAST-CANCER; IMMUNITY;
CHEMOTHERAPY; MECHANISMS; GROWTH; MICE; IMMUNOSUPPRESSION;
CYCLOPHOSPHAMIDE
AB Immune-based interventions are promising strategies to achieve long-term cancer-free survival. Fasting was previously shown to differentially sensitize tumors to chemotherapy while protecting normal cells, including hematopoietic stem and immune cells, from its toxic side effects. Here, we show that the combination of chemotherapy and a fasting-mimicking diet (FMD) increases the levels of bone marrow common lymphoid progenitor cells and cytotoxic CD8(+) tumor-infiltrating lymphocytes (TILs), leading to a major delay in breast cancer and melanoma progression. In breast tumors, this effect is partially mediated by the downregulation of the stress-responsive enzyme heme oxygenase-1 (HO-1). These data indicate that FMD cycles combined with chemotherapy can enhance T cell-dependent targeted killing of cancer cells both by stimulating the hematopoietic system and by enhancing CD8(+)-dependent tumor cytotoxicity.
C1 [Di Biase, Stefano; Lee, Changhan; Brandhorst, Sebastian; Manes, Brianna; Buono, Roberta; Cheng, Chia-Wei; Cacciottolo, Mafalda; Wei, Min; Morgan, Todd E.; Longo, Valter D.] Univ So Calif, Leonard Davis Sch Gerontol, Longev Inst, Los Angeles, CA 90089 USA.
[Di Biase, Stefano; Lee, Changhan; Brandhorst, Sebastian; Manes, Brianna; Buono, Roberta; Cheng, Chia-Wei; Cacciottolo, Mafalda; Wei, Min; Morgan, Todd E.; Longo, Valter D.] Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA.
[Martin-Montalvo, Alejandro; de Cabo, Rafael] NIA, Translat Gerontol Branch, NIH, Baltimore, MD 21224 USA.
[Longo, Valter D.] FIRC Inst Mol Oncol, IFOM, I-20139 Milan, Italy.
RP Longo, VD (reprint author), Univ So Calif, Leonard Davis Sch Gerontol, Longev Inst, Los Angeles, CA 90089 USA.; Longo, VD (reprint author), Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA.; Longo, VD (reprint author), FIRC Inst Mol Oncol, IFOM, I-20139 Milan, Italy.
EM vlongo@usc.edu
RI Martin-Montalvo, Alejandro/C-2031-2017;
OI Martin-Montalvo, Alejandro/0000-0002-3886-5355; ,
rafael/0000-0003-2830-5693; Wei, Min/0000-0002-2649-9271
FU NIH [P01 AG034906]; NCI [HHSN261201200051C]; Intramural Research Program
of the National Institute on Aging, NIH
FX This study was funded by NIH grant P01 AG034906 to V.D.L. and
subcontract HHSN261201200051C from NCI to M.W. R.d.C and A.M.M. were
funded by the Intramural Research Program of the National Institute on
Aging, NIH. We thank William Wood, Elin Lehrmann, and Yongqing Zhang for
assistance with microarray analyses. V.D.L. and T.M. have equity
interest in L-Nutra, a company developing medical food.
NR 45
TC 11
Z9 11
U1 12
U2 17
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 1535-6108
EI 1878-3686
J9 CANCER CELL
JI Cancer Cell
PD JUL 11
PY 2016
VL 30
IS 1
BP 136
EP 146
DI 10.1016/j.ccell.2016.06.005
PG 11
WC Oncology; Cell Biology
SC Oncology; Cell Biology
GA DR7JY
UT WOS:000380077100017
PM 27411588
ER
PT J
AU Meker, S
Braitbard, O
Hall, MD
Hochman, J
Tshuva, EY
AF Meker, Sigalit
Braitbard, Ori
Hall, Matthew D.
Hochman, Jacob
Tshuva, Edit Y.
TI Specific Design of Titanium(IV) Phenolato Chelates Yields Stable and
Accessible, Effective and Selective Anticancer Agents
SO CHEMISTRY-A EUROPEAN JOURNAL
LA English
DT Article
DE antitumor agents; drug design; ligand design; N,O ligands; titanium
ID HUMAN OVARIAN-CANCER; SUBSTITUTED AROMATIC RINGS; RAY-STRUCTURE
ANALYSIS; TITANOCENE DICHLORIDE; METAL-COMPLEXES; CELL-LINES;
SALAN-TITANIUM(IV) COMPLEXES; ANTIPROLIFERATIVE ACTIVITY; BIS(PHENOLATO)
LIGANDS; MECHANISTIC INSIGHTS
AB Octahedral titanium(IV) complexes of phenolato hexadentate ligands were developed and showed very high stability for days in water solutions. In vitro cytotoxicity studies showed that, whereas tetrakis(phenolato) systems are generally of low activity presumably due to inaccessibility, smaller bis(phenolato)bis(alkoxo) complexes feature high anticancer activity and accessibility even without formulations, also toward a cisplatin-resistant cell line. An all-aliphatic control complex was unstable and inactive. A leading phenolato complex also revealed: 1) high durability in fully aqueous solutions; accordingly, negligible loss of activity after preincubation for three days in medium or in serum; 2) maximal cellular accumulation and induction of apoptosis following 24-48 h of administration; 3) reduced impact on noncancerous fibroblast cells; 4) in vivo efficacy toward lymphoma cells in murine model; 5) high activity in NCI-60 panel, with average GI(50) of 4.6 +/- 2 mu m. This newly developed family of Ti-IV complexes is thus of great potential for anticancer therapy.
C1 [Meker, Sigalit; Tshuva, Edit Y.] Hebrew Univ Jerusalem, Inst Chem, IL-9190401 Jerusalem, Israel.
[Braitbard, Ori; Hochman, Jacob] Hebrew Univ Jerusalem, Alexander Silberman Inst Life Sci, Dept Cell & Dev Biol, IL-9190401 Jerusalem, Israel.
[Hall, Matthew D.] NIH, NCATS Chem Genom Ctr, Natl Ctr Adv Translat Sci, 9800 Med Ctr Dr, Rockville, MD 20850 USA.
RP Tshuva, EY (reprint author), Hebrew Univ Jerusalem, Inst Chem, IL-9190401 Jerusalem, Israel.
EM edit.tshuva@mail.huji.ac.il
RI Tshuva, Edit/B-5229-2015
OI Tshuva, Edit/0000-0001-7452-3611
FU European Council under the European Community [239603]
FX We thank Dr. Benny Bogoslaysky for crystallographic analyses, and Dr.
Karina Hazan for elemental analyses and MS measurements. Funding was
received from the European Council under the European Community's
Seventh Framework Programme (FP7/2007-2013)/ERC Grant agreement (No.
239603).
NR 59
TC 3
Z9 3
U1 10
U2 13
PU WILEY-V C H VERLAG GMBH
PI WEINHEIM
PA POSTFACH 101161, 69451 WEINHEIM, GERMANY
SN 0947-6539
EI 1521-3765
J9 CHEM-EUR J
JI Chem.-Eur. J.
PD JUL 11
PY 2016
VL 22
IS 29
BP 9986
EP 9995
DI 10.1002/chem.201601389
PG 10
WC Chemistry, Multidisciplinary
SC Chemistry
GA DS0FX
UT WOS:000380271900019
PM 27320784
ER
PT J
AU Schoborg, TA
Rusan, NM
AF Schoborg, Todd A.
Rusan, Nasser M.
TI Taking Centrioles to the Elimination Round
SO DEVELOPMENTAL CELL
LA English
DT Editorial Material
ID STARFISH OOCYTES; POLAR BODIES; CENTROSOME
AB Two recent papers published in The Journal of Cell Biology (Borrego-Pinto et al., 2016) and Science (Pimenta-Marques et al., 2016) have begun to shed light on the mechanism of centriole elimination during female oogenesis, highlighting a protective role for Polo kinase and the pericentriolar material.
C1 [Schoborg, Todd A.; Rusan, Nasser M.] NHLBI, Cell Biol & Physiol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA.
RP Rusan, NM (reprint author), NHLBI, Cell Biol & Physiol Ctr, NIH, Bldg 10, Bethesda, MD 20892 USA.
EM nasser@nih.gov
RI Rusan, Nasser/P-3511-2016
FU Intramural NIH HHS [ZIA HL006126-05]
NR 9
TC 0
Z9 0
U1 3
U2 3
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 1534-5807
EI 1878-1551
J9 DEV CELL
JI Dev. Cell
PD JUL 11
PY 2016
VL 38
IS 1
BP 10
EP 12
DI 10.1016/j.devcel.2016.06.027
PG 3
WC Cell Biology; Developmental Biology
SC Cell Biology; Developmental Biology
GA DR7IO
UT WOS:000380073400005
PM 27404354
ER
PT J
AU Chaudhari, SN
Mukherjee, M
Vagasi, AS
Bi, G
Rahman, MM
Nguyen, CQ
Paul, L
Selhub, J
Kipreos, ET
AF Chaudhari, Snehal N.
Mukherjee, Madhumati
Vagasi, Alexandra S.
Bi, Gaofeng
Rahman, Mohammad M.
Nguyen, Christine Q.
Paul, Ligi
Selhub, Jacob
Kipreos, Edward T.
TI Bacterial Folates Provide an Exogenous Signal for C. elegans Germline
Stem Cell Proliferation
SO DEVELOPMENTAL CELL
LA English
DT Article
ID CAENORHABDITIS-ELEGANS; ESCHERICHIA-COLI; RECEPTOR-ALPHA; FOLIC-ACID;
CANCER; TRANSPORTER; BIOLOGY; PROTEIN; PHOSPHORYLATION; DEFICIENCY
AB Here we describe an in vitro primary culture system for Caenorhabditis elegans germline stem cells. This culture system was used to identify a bacterial folate as a positive regulator of germ cell proliferation. Folates are a family of B-complex vitamins that function in one-carbon metabolism to allow the de novo synthesis of amino acids and nucleosides. We show that germ cell proliferation is stimulated by the folate 10-formyl-tetrahydrofolate-Glun both in vitro and in animals. Other folates that can act as vitamins to rescue folate deficiency lack this germ cell stimulatory activity. The bacterial folate precursor dihydropteroate also promotes germ cell proliferation in vitro and in vivo, despite its inability to promote one-carbon metabolism. The folate receptor homolog FOLR-1 is required for the stimulation of germ cells by 10-formyl-tetrahydrofolate-Glun and dihydropteroate. This work defines a folate and folate-related compound as exogenous signals to modulate germ cell proliferation.
C1 [Chaudhari, Snehal N.; Vagasi, Alexandra S.; Nguyen, Christine Q.; Kipreos, Edward T.] Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA.
[Mukherjee, Madhumati; Rahman, Mohammad M.] Univ Georgia, Dept Genet, Athens, GA 30602 USA.
[Bi, Gaofeng; Paul, Ligi; Selhub, Jacob] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA.
[Rahman, Mohammad M.] NIDDK, LCMB, Bethesda, MD 20892 USA.
RP Kipreos, ET (reprint author), Univ Georgia, Dept Cellular Biol, Athens, GA 30602 USA.
EM ekipreos@uga.edu
FU NIH Office of Research Infrastructure Programs [P40 OD010440]; NSF
[MCB-1138454]; NIH/NIGMS [R01 GM074212]; United States Department of
Agriculture [51520-008-04S]
FX We thank M.J. McEachern and members of the Kipreos laboratory for
critical comments on the manuscript, as well as M.C. Zetka, D.B.
Hausman, and S. Dougan for reagents or resources. Some C. elegans
strains were provided by the CGC, which is funded by NIH Office of
Research Infrastructure Programs (P40 OD010440). This work was supported
by grants from NSF (MCB-1138454) and NIH/NIGMS (R01 GM074212) (E.T.K.),
and support from United States Department of Agriculture cooperative
agreement 51520-008-04S (J.S.). Any opinions, findings, conclusion, or
recommendations expressed in this publication are those of the authors
and do not necessarily reflect the view of the United States Department
of Agriculture.
NR 60
TC 0
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U1 10
U2 10
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 1534-5807
EI 1878-1551
J9 DEV CELL
JI Dev. Cell
PD JUL 11
PY 2016
VL 38
IS 1
BP 33
EP 46
DI 10.1016/j.devcel.2016.06.013
PG 14
WC Cell Biology; Developmental Biology
SC Cell Biology; Developmental Biology
GA DR7IO
UT WOS:000380073400008
PM 27404357
ER
PT J
AU Choi, CH
Chung, JY
Chung, EJ
Sears, JD
Lee, JW
Bae, DS
Hewitt, SM
AF Choi, Chel Hun
Chung, Joon-Yong
Chung, Eun Joo
Sears, John D.
Lee, Jeong-Won
Bae, Duk-Soo
Hewitt, Stephen M.
TI Prognostic significance of annexin A2 and annexin A4 expression in
patients with cervical cancer
SO BMC CANCER
LA English
DT Article
DE ANXA2; ANXA4; Prognosis; Survival; Uterine cervical neoplasms
ID CLEAR-CELL CARCINOMA; HUMAN-PAPILLOMAVIRUS; HEPATOCELLULAR-CARCINOMA;
PROSTATE-CANCER; ENHANCED EXPRESSION; II EXPRESSION; TENASCIN-C;
PROGRESSION; IV; MIGRATION
AB Background: The annexins (ANXs) have diverse roles in tumor development and progression, however, their clinical significance in cervical cancer has not been elucidated. The present study was to investigate the clinical significance of annexin A2 (ANXA2) and annexin A4 (ANXA4) expression in cervical cancer.
Methods: ANXA2 and ANXA4 immunohistochemical staining were performed on a cervical cancer tissue microarray consisting of 46 normal cervical epithelium samples and 336 cervical cancer cases and compared the data with clinicopathological variables, including the survival of cervical cancer patients.
Results: ANXA2 expression was lower in cancer tissue (p = 0.002), whereas ANXA4 staining increased significantly in cancer tissues (p < 0.001). ANXA2 expression was more prominent in squamous cell carcinoma (p < 0.001), whereas ANXA4 was more highly expressed in adeno/adenosquamous carcinoma (p < 0.001). ANXA2 overexpression was positively correlated with advanced cancer phenotypes, whereas ANXA4 expression was associated with resistance to radiation with or without chemotherapy (p = 0.029). Notably, high ANXA2 and ANXA4 expression was significantly associated with shorter disease-free survival (p = 0.004 and p = 0.033, respectively). Multivariate analysis indicated that ANXA2+ (HR = 2.72, p = 0.003) and ANXA2+/ANXA4+ (HR = 2.69, p = 0.039) are independent prognostic factors of disease-free survival in cervical cancer. Furthermore, a random survival forest model using combined ANXA2, ANXA4, and clinical variables resulted in improved predictive power (mean C-index, 0.76) compared to that of clinical-variable-only models (mean C-index, 0.70) (p = 0.006).
Conclusions: These findings indicate that detecting ANXA2 and ANXA4 expression may aid the evaluation of cervical carcinoma prognosis.
C1 [Choi, Chel Hun; Chung, Joon-Yong; Sears, John D.; Hewitt, Stephen M.] NCI, Expt Pathol Lab, Pathol Lab, Ctr Canc Res,NIH, MSC 1500, Bethesda, MD 20892 USA.
[Choi, Chel Hun; Lee, Jeong-Won; Bae, Duk-Soo] Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Obstet & Gynecol, 50 Irwon Dong, Seoul 135710, South Korea.
[Chung, Eun Joo] NCI, Radiat Oncol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
RP Hewitt, SM (reprint author), NCI, Expt Pathol Lab, Pathol Lab, Ctr Canc Res,NIH, MSC 1500, Bethesda, MD 20892 USA.; Bae, DS (reprint author), Sungkyunkwan Univ, Sch Med, Samsung Med Ctr, Dept Obstet & Gynecol, 50 Irwon Dong, Seoul 135710, South Korea.
EM ds123.bae@samsung.com; genejock@helix.nih.gov
RI lee, jw/O-6237-2014;
OI Chung, Joon-Yong/0000-0001-5041-5982
FU Basic Science Research Program through the National Research Foundation
of Korea (NRF) - Ministry of Education, Republic of Korea
[2013R1A1A2013629]; Intramural Research Program of the NIH, National
Cancer Institute, Center for Cancer Research
FX This study was supported in part by a grant from the Basic Science
Research Program through the National Research Foundation of Korea (NRF)
funded by the Ministry of Education, Republic of Korea
(2013R1A1A2013629) and the Intramural Research Program of the NIH,
National Cancer Institute, Center for Cancer Research.
NR 52
TC 0
Z9 0
U1 3
U2 5
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1471-2407
J9 BMC CANCER
JI BMC Cancer
PD JUL 11
PY 2016
VL 16
AR 448
DI 10.1186/s12885-016-2459-y
PG 11
WC Oncology
SC Oncology
GA DR6MD
UT WOS:000380015100001
PM 27402115
ER
PT J
AU Goff, SL
Dudley, ME
Citrin, DE
Somerville, RP
Wunderlich, JR
Danforth, DN
Zlott, DA
Yang, JC
Sherry, RM
Kammula, US
Klebanoff, CA
Hughes, MS
Restifo, NP
Langhan, MM
Shelton, TE
Lu, L
Kwong, MLM
Ilyas, S
Klemen, ND
Payabyab, EC
Morton, KE
Toomey, MA
Steinberg, SM
White, DE
Rosenberg, SA
AF Goff, Stephanie L.
Dudley, Mark E.
Citrin, Deborah E.
Somerville, Robert P.
Wunderlich, John R.
Danforth, David N.
Zlott, Daniel A.
Yang, James C.
Sherry, Richard M.
Kammula, Udai S.
Klebanoff, Christopher A.
Hughes, Marybeth S.
Restifo, Nicholas P.
Langhan, Michelle M.
Shelton, Thomas E.
Lu, Lily
Kwong, Mei Li M.
Ilyas, Sadia
Klemen, Nicholas D.
Payabyab, Eden C.
Morton, Kathleen E.
Toomey, Mary Ann
Steinberg, Seth M.
White, Donald E.
Rosenberg, Steven A.
TI Randomized, Prospective Evaluation Comparing Intensity of
Lymphodepletion Before Adoptive Transfer of Tumor-Infiltrating
Lymphocytes for Patients With Metastatic Melanoma
SO JOURNAL OF CLINICAL ONCOLOGY
LA English
DT Article
ID CELL TRANSFER THERAPY; T-CELLS; UNTREATED MELANOMA; IPILIMUMAB; CANCER;
INTERLEUKIN-2; CHEMOTHERAPY; NIVOLUMAB; ANTI-PD-1; EFFICACY
AB Purpose
Adoptive cell transfer, the infusion of large numbers of activated autologous lymphocytes, can mediate objective tumor regression in a majority of patients with metastatic melanoma (52 of 93; 56%). Addition and intensification of total body irradiation (TBI) to the preparative lymphodepleting chemotherapy regimen in sequential trials improved objective partial and complete response (CR) rates. Here, we evaluated the importance of adding TBI to the adoptive transfer of tumor-infiltrating lymphocytes (TIL) in a randomized fashion.
Patients and Methods
A total of 101 patients with metastatic melanoma, including 76 patients with M1c disease, were randomly assigned to receive nonmyeloablative chemotherapy with or without 1,200 cGy TBI before transfer of tumor-infiltrating lymphcytes. Primary end points were CR rate (as defined by Response Evaluation Criteria in Solid Tumors v1.0) and overall survival (OS). Clinical and laboratory data were analyzed for correlates of response.
Results
CR rates were 24% in both groups (12 of 50 v 12 of 51), and OS was also similar (median OS, 38.2 v 36.6 months; hazard ratio, 1.11; 95% CI, 0.65 to 1.91; P=.71). Thrombotic microangiopathy was an adverse event unique to the TBI arm and occurred in 13 of 48 treated patients. With a median potential follow-up of 40.9 months, only one of 24 patients who achieved a CR recurred.
Conclusion
Adoptive cell transfer can mediate durable complete regressions in 24% of patients with metastatic melanoma, with median survival. 3 years. Results were similar using chemotherapy preparative regimens with or without addition of TBI. (C) 2016 by American Society of Clinical Oncology
C1 [Goff, Stephanie L.; Citrin, Deborah E.; Somerville, Robert P.; Wunderlich, John R.; Danforth, David N.; Yang, James C.; Sherry, Richard M.; Kammula, Udai S.; Klebanoff, Christopher A.; Hughes, Marybeth S.; Restifo, Nicholas P.; Langhan, Michelle M.; Shelton, Thomas E.; Lu, Lily; Kwong, Mei Li M.; Ilyas, Sadia; Klemen, Nicholas D.; Payabyab, Eden C.; Morton, Kathleen E.; Toomey, Mary Ann; Steinberg, Seth M.; White, Donald E.; Rosenberg, Steven A.] NCI, NIH, Bethesda, MD 20892 USA.
[Zlott, Daniel A.] NIH, Ctr Clin, Bethesda, MD 20892 USA.
[Dudley, Mark E.] Novartis Inst BioMed Res, Cambridge, MA USA.
RP Goff, SL (reprint author), NCI, Surg Branch, NIH, 10 Ctr Dr,Rm 3-3940, Bethesda, MD 20892 USA.
EM stephanie.goff@nih.gov
FU Center for Cancer Research at the National Cancer Institute of the US
National Institutes of Health
FX Supported by the Center for Cancer Research at the National Cancer
Institute of the US National Institutes of Health, by a generous gift
from the Adelson Medical Research Foundation, and through a cooperative
research and development agreement with Lion Biotechnologies.
NR 35
TC 11
Z9 11
U1 1
U2 8
PU AMER SOC CLINICAL ONCOLOGY
PI ALEXANDRIA
PA 2318 MILL ROAD, STE 800, ALEXANDRIA, VA 22314 USA
SN 0732-183X
EI 1527-7755
J9 J CLIN ONCOL
JI J. Clin. Oncol.
PD JUL 10
PY 2016
VL 34
IS 20
BP 2389
EP U145
DI 10.1200/JCO.2016.66.7220
PG 16
WC Oncology
SC Oncology
GA DQ3TW
UT WOS:000379127400010
PM 27217459
ER
PT J
AU Beumer, JH
Tawbi, H
Ivy, SP
AF Beumer, Jan H.
Tawbi, Hussein
Ivy, S. Percy
TI Harmonization of Renal Function Assessment Is Needed Throughout the
Whole Process of Anticancer Drug Development Reply
SO JOURNAL OF CLINICAL ONCOLOGY
LA English
DT Letter
ID CREATININE
C1 [Beumer, Jan H.] Univ Pittsburgh, Pittsburgh, PA USA.
[Tawbi, Hussein] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA.
[Ivy, S. Percy] NCI, Bethesda, MD 20892 USA.
RP Beumer, JH (reprint author), Univ Pittsburgh, Pittsburgh, PA USA.
OI Beumer, Jan/0000-0002-8978-9401
FU NCI NIH HHS [P30 CA047904, UM1 CA186690]
NR 11
TC 0
Z9 0
U1 0
U2 0
PU AMER SOC CLINICAL ONCOLOGY
PI ALEXANDRIA
PA 2318 MILL ROAD, STE 800, ALEXANDRIA, VA 22314 USA
SN 0732-183X
EI 1527-7755
J9 J CLIN ONCOL
JI J. Clin. Oncol.
PD JUL 10
PY 2016
VL 34
IS 20
BP 2430
EP U195
DI 10.1200/JCO.2016.67.2790
PG 3
WC Oncology
SC Oncology
GA DQ3TW
UT WOS:000379127400017
PM 27185845
ER
PT J
AU Shi, YH
Posse, V
Zhu, XF
Hyvarinen, AK
Jacobs, HT
Falkenberg, M
Gustafsson, CM
AF Shi, Yonghong
Posse, Viktor
Zhu, Xuefeng
Hyvarinen, Anne K.
Jacobs, Howard T.
Falkenberg, Maria
Gustafsson, Claes M.
TI Mitochondrial transcription termination factor 1 directs polar
replication fork pausing
SO NUCLEIC ACIDS RESEARCH
LA English
DT Article
ID DNA-POLYMERASE-GAMMA; IN-VITRO; BINDING PROTEIN; RNA-POLYMERASE;
RIBOSOMAL-RNA; FACTOR MTERF; LAGGING-STRAND; HUMAN MTDNA; TWINKLE; CELLS
AB During replication of nuclear ribosomal DNA (rDNA), clashes with the transcription apparatus can cause replication fork collapse and genomic instability. To avoid this problem, a replication fork barrier protein is situated downstream of rDNA, there preventing replication in the direction opposite rDNA transcription. A potential candidate for a similar function in mitochondria is the mitochondrial transcription termination factor 1 (MTERF1, also denoted mTERF), which binds to a sequence just downstream of the ribosomal transcription unit. Previous studies have shown that MTERF1 prevents antisense transcription over the ribosomal RNA genes, a process which we here show to be independent of the transcription elongation factor TEFM. Importantly, we now demonstrate that MTERF1 arrests mitochondrial DNA (mtDNA) replication with distinct polarity. The effect is explained by the ability of MTERF1 to act as a directional contrahelicase, blocking mtDNA unwinding by the mitochondrial helicase TWINKLE. This conclusion is also supported by in vivo evidence that MTERF1 stimulates TWINKLE pausing. We conclude that MTERF1 can direct polar replication fork arrest in mammalian mitochondria.
C1 [Shi, Yonghong; Posse, Viktor; Zhu, Xuefeng; Falkenberg, Maria; Gustafsson, Claes M.] Univ Gothenburg, Inst Biomed, POB 440, SE-40530 Gothenburg, Sweden.
[Shi, Yonghong; Zhu, Xuefeng] NHLBI, Ctr Mol Med, NIH, Bethesda, MD 20892 USA.
[Hyvarinen, Anne K.; Jacobs, Howard T.] Univ Tampere, BioMediTech, FI-33014 Tampere, Finland.
[Hyvarinen, Anne K.; Jacobs, Howard T.] Univ Tampere, Tampere Univ Hosp, FI-33014 Tampere, Finland.
[Jacobs, Howard T.] Univ Helsinki, Inst Biotechnol, FI-00014 Helsinki, Finland.
RP Falkenberg, M; Gustafsson, CM (reprint author), Univ Gothenburg, Inst Biomed, POB 440, SE-40530 Gothenburg, Sweden.
EM maria.falkenberg@medkem.gu.se; claes.gustafsson@medkem.gu.se
RI shi, yonghong/E-8254-2017
OI shi, yonghong/0000-0002-5265-7982
FU Swedish Research Council [2013-3621, 2012-2583]; Swedish Cancer
Foundation; European Research Council [261248, 268897]; IngaBritt and
Arne Lundberg foundation; Knut and Alice Wallenbergs foundation; Academy
of Finland (FinMIT Centre of Excellence) [118654, 256615]
FX Swedish Research Council grants [2013-3621 to M.F., 2012-2583 to
C.M.G.]; Swedish Cancer Foundation (to M.F. and C.M.G.); European
Research Council [261248 to M.F., 268897 to C.G.]; IngaBritt and Arne
Lundberg foundation (to M.F.); Knut and Alice Wallenbergs foundation (to
M.F. and C.M.G.); Academy of Finland (FinMIT Centre of Excellence 118654
and Professorship 256615 to H.T.J.). Funding for open access charge:
European Research Council.
NR 53
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U1 4
U2 4
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0305-1048
EI 1362-4962
J9 NUCLEIC ACIDS RES
JI Nucleic Acids Res.
PD JUL 8
PY 2016
VL 44
IS 12
BP 5732
EP 5742
DI 10.1093/nar/gkw302
PG 11
WC Biochemistry & Molecular Biology
SC Biochemistry & Molecular Biology
GA DT0ZG
UT WOS:000381210900024
PM 27112570
ER
PT J
AU Thomas, MA
Nyanhete, T
Tuero, I
Venzon, D
Robert-Guroff, M
AF Thomas, Michael A.
Nyanhete, Tinashe
Tuero, Iskra
Venzon, David
Robert-Guroff, Marjorie
TI Beyond Oncolytics: E1B55K-Deleted Adenovirus as a Vaccine Delivery
Vector
SO PLOS ONE
LA English
DT Article
ID HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL RESPONSES; RHESUS MACAQUES;
PROTECTIVE EFFICACY; IMMUNE-RESPONSES; E1B PROTEINS; CHALLENGE;
IMMUNIZATION; EXPRESSION; REPLICATION
AB Type 5 human adenoviruses (Ad5) deleted of genes encoding the early region 1B 55-kDa (E1B55K) protein including Onyx-015 (dl1520) and H101 are best known for their oncolytic potential. As a vaccine vector the E1B55K deletion may allow for the insertion of a transgene nearly 1,000 base pairs larger than now possible. This has the potential of extending the application for which the vectors are clinically known. However, the immune priming ability of E1B55K-deleted vectors is unknown, undermining our ability to gauge their usefulness in vaccine applications. For this reason, we created an E1B55K-deleted Ad5 vector expressing full-length single chain HIV(BaL)gp120 attached to a flexible linker and the first two domains of rhesus CD4 (rhFLSC) in exchange for the E3 region. In cell-based experiments the E1B55K-deleted vector promoted higher levels of innate immune signals including chemokines, cytokines, and the NKG2D ligands MIC A/B compared to an E1B55K wild-type vector expressing the same immunogen. Based on these results we evaluated the immune priming ability of the E1B55K-deleted vector in mice. The E1B55K-deleted vector promoted similar levels of Ad5-, HIVgp120, and rhFLSC-specific cellular and humoral immune responses as the E1B55K wild-type vector. In pre-clinical HIV-vaccine studies the wild-type vector has been employed as part of a very effective prime-boost strategy. This study demonstrates that E1B55K-deleted adenoviruses may serve as effective vaccine delivery vectors.
C1 [Thomas, Michael A.; Nyanhete, Tinashe; Tuero, Iskra; Robert-Guroff, Marjorie] NCI, Sect Immune Biol Retroviral Infect, Vaccine Branch, NIH, Bethesda, MD USA.
[Venzon, David] NCI, Biostat & Data Management Sect, NIH, Bethesda, MD USA.
[Thomas, Michael A.] Howard Univ, Dept Biol, Washington, DC USA.
[Nyanhete, Tinashe] Duke Univ, Durham, NC USA.
RP Thomas, MA; Robert-Guroff, M (reprint author), NCI, Sect Immune Biol Retroviral Infect, Vaccine Branch, NIH, Bethesda, MD USA.; Thomas, MA (reprint author), Howard Univ, Dept Biol, Washington, DC USA.
EM michael.thomas1@howard.edu; guroffm@mail.nih.gov
FU Pathway to Independence Award (K99) [1K99AI114379 - 01]; Intramural
Research Program of the National Institutes of Health, National Cancer
Institute
FX This work was supported by a Pathway to Independence Award (K99) Grant
Number 1K99AI114379 - 01 to MAT and by the Intramural Research Program
of the National Institutes of Health, National Cancer Institute to MRG.
NR 29
TC 0
Z9 0
U1 5
U2 5
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 8
PY 2016
VL 11
IS 7
AR e0158505
DI 10.1371/journal.pone.0158505
PG 17
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR6IK
UT WOS:000380005400072
PM 27391605
ER
PT J
AU Valm, AM
Oldenbourg, R
Borisy, GG
AF Valm, Alex M.
Oldenbourg, Rudolf
Borisy, Gary G.
TI Multiplexed Spectral Imaging of 120 Different Fluorescent Labels
SO PLOS ONE
LA English
DT Article
ID ARCHITECTURE; MICROSCOPY; PROTEINS
AB The number of fluorescent labels that can unambiguously be distinguished in a single image when acquired through band pass filters is severely limited by the spectral overlap of available fluorophores. The recent development of spectral microscopy and the application of linear unmixing algorithms to spectrally recorded image data have allowed simultaneous imaging of fluorophores with highly overlapping spectra. However, the number of distinguishable fluorophores is still limited by the unavoidable decrease in signal to noise ratio when fluorescence signals are fractionated over multiple wavelength bins. Here we present a spectral image analysis algorithm to greatly expand the number of distinguishable objects labeled with binary combinations of fluorophores. Our algorithm utilizes a priori knowledge about labeled specimens and imposes a binary label constraint on the unmixing solution. We have applied our labeling and analysis strategy to identify microbes labeled by fluorescence in situ hybridization and here demonstrate the ability to distinguish 120 differently labeled microbes in a single image.
C1 [Valm, Alex M.; Oldenbourg, Rudolf] Marine Biol Lab, Woods Hole, MA 02543 USA.
[Valm, Alex M.; Oldenbourg, Rudolf] Brown Univ, Providence, RI 02912 USA.
[Borisy, Gary G.] Forsyth Inst, Cambridge, MA USA.
[Valm, Alex M.] NICHHD, NIH, NIH NICHD, Bethesda, MD 20892 USA.
RP Valm, AM (reprint author), Marine Biol Lab, Woods Hole, MA 02543 USA.; Valm, AM (reprint author), Brown Univ, Providence, RI 02912 USA.; Valm, AM (reprint author), NICHHD, NIH, NIH NICHD, Bethesda, MD 20892 USA.
EM Alex.Valm@nih.gov
FU Alfred P. Sloan Foundation [2007-3-13]; National Institutes of Health
from the National Institute of Dental and Craniofacial Research (NIDCR)
[1RC1-DE020630]; National Institutes of Health from NIDCR
[1F31-DE019576]
FX This work was supported by Grant 2007-3-13 from the Alfred P. Sloan
Foundation (to GGB), National Institutes of Health Grant 1RC1-DE020630
from the National Institute of Dental and Craniofacial Research (NIDCR)
(to GGB) and by National Institutes of Health Fellowship 1F31-DE019576
from NIDCR (to AMV). The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the
manuscript.
NR 17
TC 0
Z9 0
U1 2
U2 3
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 8
PY 2016
VL 11
IS 7
AR e0158495
DI 10.1371/journal.pone.0158495
PG 17
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR6IK
UT WOS:000380005400069
PM 27391327
ER
PT J
AU Li, MH
Simonetti, FL
Goncearenco, A
Panchenko, AR
AF Li, Minghui
Simonetti, Franco L.
Goncearenco, Alexander
Panchenko, Anna R.
TI MutaBind estimates and interprets the effects of sequence variants on
protein-protein interactions
SO NUCLEIC ACIDS RESEARCH
LA English
DT Article
ID MISSENSE MUTATIONS; BINDING-AFFINITY; FREE-ENERGIES; DYNAMICS;
STABILITY; MODELS; IMPACT
AB Proteins engage in highly selective interactions with their macromolecular partners. Sequence variants that alter protein binding affinity may cause significant perturbations or complete abolishment of function, potentially leading to diseases. There exists a persistent need to develop a mechanistic understanding of impacts of variants on proteins. To address this need we introduce a new computational method MutaBind to evaluate the effects of sequence variants and disease mutations on protein interactions and calculate the quantitative changes in binding affinity. The MutaBind method uses molecular mechanics force fields, statistical potentials and fast side-chain optimization algorithms. The MutaBind server maps mutations on a structural protein complex, calculates the associated changes in binding affinity, determines the deleterious effect of a mutation, estimates the confidence of this prediction and produces a mutant structural model for download. MutaBind can be applied to a large number of problems, including determination of potential driver mutations in cancer and other diseases, elucidation of the effects of sequence variants on protein fitness in evolution and protein design. MutaBind is available at http://www.ncbi.nlm.nih.gov/projects/mutabind/.
C1 [Li, Minghui; Goncearenco, Alexander; Panchenko, Anna R.] NIH, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA.
[Simonetti, Franco L.] Inst Leloir Fdn, C1405BWE, Buenos Aires, DF, Argentina.
RP Panchenko, AR (reprint author), NIH, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA.
EM panch@ncbi.nlm.nih.gov
RI Goncearenco, Alexander/M-3946-2015
OI Goncearenco, Alexander/0000-0002-9738-7146
FU Intramural Research Program of the National Library of Medicine at the
U.S. National Institutes of Health; Argentine Government/BEC.AR;
Argentine Fulbright Commission; National Science Foundation (NSF)
[PHY11-25915]; National Institutes of Health
FX Intramural Research Program of the National Library of Medicine at the
U.S. National Institutes of Health (to M.L., A.G., A.R.P.); Argentine
Government/BEC.AR (to F.L.S.); Argentine Fulbright Commission (to
F.L.S.); National Science Foundation [NSF PHY11-25915, in part]. Funding
for open access charge: National Institutes of Health.
NR 32
TC 3
Z9 3
U1 2
U2 5
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0305-1048
EI 1362-4962
J9 NUCLEIC ACIDS RES
JI Nucleic Acids Res.
PD JUL 8
PY 2016
VL 44
IS W1
BP W494
EP W501
DI 10.1093/nar/gkw374
PG 8
WC Biochemistry & Molecular Biology
SC Biochemistry & Molecular Biology
GA DR3FA
UT WOS:000379786800081
PM 27150810
ER
PT J
AU Hao, M
Bryant, SH
Wang, YL
AF Hao, Ming
Bryant, Stephen H.
Wang, Yanli
TI Cheminformatics analysis of the AR agonist and antagonist datasets in
PubChem
SO JOURNAL OF CHEMINFORMATICS
LA English
DT Article
ID MOLECULAR PAIR ANALYSIS; ACTIVITY CLIFFS; SYSTEMATIC IDENTIFICATION;
EFFICIENT ALGORITHM; BIOASSAY DATA; DATA SETS; INHIBITORS; DRUGS;
SCAFFOLDS
AB Background: As one of the largest publicly accessible databases for hosting chemical structures and biological activities, PubChem has been processing bioassay submissions from the community since 2004. With the increase in volume for the deposited data in PubChem, the diversity and wealth of information content also grows. Recently, the Tox21 program, has deposited a series of pairwise data in PubChem regarding to different mechanism of actions (MOA), such as androgen receptor (AR) agonist and antagonist datasets, to study cell toxicity. To the best of our knowledge, little work has been reported from cheminformatics study for these especially pairwise datasets, which may provide insight into the mechanism of actions of the compounds and relationship between chemical structures and functions, as well as guidance for lead compound selection and optimization. Thus, to fill the gap, we performed a comprehensive cheminformatics analysis, including scaffold analysis, matched molecular pair (MMP) analysis as well as activity cliff analysis to investigate the structural characteristics and discontinued structure-activity relationship of the individual dataset (i.e., AR agonist dataset or AR antagonist dataset) and the combined dataset (i.e., the common compounds between the AR agonist and antagonist datasets).
Results: Scaffolds associated only with potential agonists or antagonists were identified. MMP-based activity cliffs, as well as a small group of compounds with dual MOA reported were recognized and analyzed. Moreover, MOA-cliff, a novel concept, was proposed to indicate one pair of structurally similar molecules which exhibit opposite MOA.
Conclusions: Cheminformatics methods were successfully applied to the pairwise AR datasets and the identified molecular scaffold characteristics, MMPs as well as activity cliffs might provide useful information when designing new lead compounds for the androgen receptor.
C1 [Hao, Ming; Bryant, Stephen H.; Wang, Yanli] NIH, Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA.
RP Wang, YL (reprint author), NIH, Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA.
EM ywang@ncbi.nlm.nih.gov
FU Intramural Research Program of the NIH, National Library of Medicine
FX This research was supported by the Intramural Research Program of the
NIH, National Library of Medicine.
NR 33
TC 0
Z9 0
U1 3
U2 3
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1758-2946
J9 J CHEMINFORMATICS
JI J. Cheminformatics
PD JUL 8
PY 2016
VL 8
AR 37
DI 10.1186/s13321-016-0150-6
PG 13
WC Chemistry, Multidisciplinary; Computer Science, Information Systems;
Computer Science, Interdisciplinary Applications
SC Chemistry; Computer Science
GA DQ6OB
UT WOS:000379323500001
PM 27398098
ER
PT J
AU Deleage, C
Schuetz, A
Alvord, WG
Johnston, L
Hao, XP
Morcock, DR
Rerknimitr, R
Fletcher, JLK
Puttamaswin, S
Phanuphak, N
Dewar, R
McCune, JM
Sereti, I
Robb, M
Kim, JH
Schacker, TW
Hunt, P
Lifson, JD
Ananworanich, J
Estes, JD
AF Deleage, Claire
Schuetz, Alexandra
Alvord, W. Gregory
Johnston, Leslie
Hao, Xing-Pei
Morcock, David R.
Rerknimitr, Rungsun
Fletcher, James L. K.
Puttamaswin, Suwanna
Phanuphak, Nittaya
Dewar, Robin
McCune, Joseph M.
Sereti, Irini
Robb, Merlin
Kim, Jerome H.
Schacker, Timothy W.
Hunt, Peter
Lifson, Jeffrey D.
Ananworanich, Jintanat
Estes, Jacob D.
CA RV254 SEARCH Study Grp
RV304 SEARCH Study Grp
TI Impact of early cART in the gut during acute HIV infection
SO JCI INSIGHT
LA English
DT Article
ID HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL-ACTIVATION; MUCOSAL IMMUNE
RECONSTITUTION; ACTIVE ANTIRETROVIRAL THERAPY; LYMPHOID-TISSUE;
MICROBIAL TRANSLOCATION; LENTIVIRAL INFECTIONS; SYSTEMIC INFLAMMATION;
EPITHELIAL BARRIER; VIRAL RESERVOIRS
AB Early after HIV infection there is substantial depletion of CD4(+) T cells in the gastrointestinal (GI) tract lamina propria (LP), with associated epithelial barrier damage, leading to microbial translocation and systemic inflammation and immune activation. In this study, we analyzed these early events in the GI tract in a cohort of Thai acute HIV-infected patients and determined the effect of early combination antiretroviral treatment (cART). HIV-uninfected and chronically and acutely HIV-infected patients at different Fiebig stages (I-V) underwent colonic biopsies and then received cART. Immunohistochemistry and quantitative image analysis were performed on cross-sectional and longitudinal colon biopsy specimens (day 0 to week 96) to measure GI tract damage (infiltration of polymorphonuclear cells), inflammation (Mx1, TNF-alpha), immune activation (Ki-67), and the CD4(+) T cell population in the LP. The magnitude of GI tract damage, immune activation, and inflammation was significantly increased, with significantly depleted CD4(+) T cells in the LP in all acutely infected groups prior to cART compared with HIV-uninfected control participants. While most patients treated during acute infection resolved GI tract inflammation and immune activation back to baseline levels after 24 weeks of cART, most acutely infected participants did not restore their CD4(+) T cells after 96 weeks of cART.
C1 [Deleage, Claire; Johnston, Leslie; Morcock, David R.; Lifson, Jeffrey D.; Estes, Jacob D.] Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, AIDS & Canc Virus Program, Frederick, MD USA.
[Schuetz, Alexandra] Armed Forces Res Inst Med Sci, Dept Retrovirol, Bangkok, Thailand.
[Schuetz, Alexandra; Robb, Merlin; Kim, Jerome H.; Ananworanich, Jintanat] Henry M Jackson Fdn Adv Mil Med, US Mil HIV Res Program, Bethesda, MD USA.
[Alvord, W. Gregory] Data Management Serv Inc, Stat Consulting, Frederick, MD USA.
[Hao, Xing-Pei] Leidos Biomedical Res Inc, Frederick Natl Lab Canc Res, Pathol & Histotechnol Lab, Frederick, MD USA.
[Rerknimitr, Rungsun] Chulalongkorn Univ, Bangkok, Thailand.
[Fletcher, James L. K.; Puttamaswin, Suwanna; Phanuphak, Nittaya; Kim, Jerome H.; Ananworanich, Jintanat] SEARCH, Bangkok, Thailand.
[Fletcher, James L. K.; Puttamaswin, Suwanna; Phanuphak, Nittaya] Thai Red Cross AIDS Res Ctr, Bangkok, Thailand.
[Dewar, Robin] NCI, Virus Isolat & Serol Lab, Appl & Dev Res Directorate, Sci Applicat Int Corp,Frederick Inc,Frederick Can, Frederick, MD 21701 USA.
[McCune, Joseph M.] UCSF, Dept Med, Div Expt Med, San Francisco, CA USA.
[Sereti, Irini] NIAID, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Kim, Jerome H.] Int Vaccine Inst, Seoul, South Korea.
[Schacker, Timothy W.] Univ Minnesota, Dept Med, Box 736 UMHC, Minneapolis, MN 55455 USA.
[Hunt, Peter] UCSF, Dept Med, Posit Hlth Program, San Francisco, CA USA.
RP Estes, JD (reprint author), Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, 1050 Boyles St,Bldg 535,Rm 411, Frederick, MD 21702 USA.
EM estesj@mail.nih.gov
NR 71
TC 2
Z9 2
U1 1
U2 1
PU AMER SOC CLINICAL INVESTIGATION INC
PI ANN ARBOR
PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA
SN 2379-3708
J9 JCI INSIGHT
JI JCI Insight
PD JUL 7
PY 2016
VL 1
IS 10
AR e87065
DI 10.1172/jci.insight.87065
PG 18
WC Medicine, Research & Experimental
SC Research & Experimental Medicine
GA EB1LL
UT WOS:000387113300015
ER
PT J
AU Myles, IA
Williams, KW
Reckhow, JD
Jammeh, ML
Pincus, NB
Sastalla, I
Saleem, D
Stone, KD
Datta, SK
AF Myles, Ian A.
Williams, Kelli W.
Reckhow, Jensen D.
Jammeh, Momodou L.
Pincus, Nathan B.
Sastalla, Inka
Saleem, Danial
Stone, Kelly D.
Datta, Sandip K.
TI Transplantation of human skin microbiota in models of atopic dermatitis
SO JCI INSIGHT
LA English
DT Article
ID DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; THYMIC STROMAL
LYMPHOPOIETIN; TRANSEPIDERMAL WATER-LOSS; STAPHYLOCOCCUS-AUREUS;
METHICILLIN-RESISTANT; GENE POLYMORPHISM; DOUBLE-BLIND; VITAMIN-D;
BARRIER
AB Atopic dermatitis (AD) is characterized by reduced barrier function, reduced innate immune activation, and susceptibility to Staphylococcus aureus. Host susceptibility factors are suggested by monogenic disorders associated with AD-like phenotypes and can be medically modulated. S. aureus contributes to AD pathogenesis and can be mitigated by antibiotics and bleach baths. Recent work has revealed that the skin microbiome differs significantly between healthy controls and patients with AD, including decreased Gram-negative bacteria in AD. However, little is known about the potential therapeutic benefit of microbiome modulation. To evaluate whether parameters of AD pathogenesis are altered after exposure to different culturable Gram-negative bacteria (CGN) collected from human skin, CGN were collected from healthy controls and patients with AD. Then, effects on cellular and culture-based models of immune, epithelial, and bacterial function were evaluated. Representative strains were evaluated in the MC903 mouse model of AD. We found that CGN taken from healthy volunteers but not from patients with AD were associated with enhanced barrier function, innate immunity activation, and control of S. aureus. Treatment with CGN from healthy controls improved outcomes in a mouse model of AD. These findings suggest that a live-biotherapeutic approach may hold promise for treatment of patients with AD.
C1 [Myles, Ian A.; Williams, Kelli W.; Reckhow, Jensen D.; Jammeh, Momodou L.; Pincus, Nathan B.; Sastalla, Inka; Saleem, Danial; Datta, Sandip K.] NIAID, Bacterial Pathogenesis Unit, Lab Clin Infect Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Stone, Kelly D.] NIAID, Lab Allerg Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
RP Myles, IA; Datta, SK (reprint author), 9000 Rockville Pike,Bldg 33,Room 2W10A, Bethesda, MD 20892 USA.
EM mylesi@niaid.nih.gov; dattas@niaid.nih.gov
OI Datta, Sandip/0000-0003-0243-7815
NR 53
TC 4
Z9 4
U1 3
U2 3
PU AMER SOC CLINICAL INVESTIGATION INC
PI ANN ARBOR
PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA
SN 2379-3708
J9 JCI INSIGHT
JI JCI Insight
PD JUL 7
PY 2016
VL 1
IS 10
AR e86955
DI 10.1172/jci.insight.86955
PG 10
WC Medicine, Research & Experimental
SC Research & Experimental Medicine
GA EB1LL
UT WOS:000387113300013
ER
PT J
AU Liu, CT
Raghavan, S
Maruthur, N
Kabagambe, EK
Hong, J
Ng, MCY
Hivert, MF
Lu, YC
An, P
Bentley, AR
Drolet, AM
Gaulton, KJ
Guo, XQ
Armstrong, LL
Irvin, MR
Li, M
Lipovich, L
Rybin, DV
Taylor, KD
Agyemang, C
Palmer, ND
Cade, BE
Chen, WM
Dauriz, M
Delaney, JAC
Edwards, TL
Evans, DS
Evans, MK
Lange, LA
Leong, A
Liu, JM
Liu, YM
Nayak, U
Patel, SR
Porneala, BC
Rasmussen-Torvik, LJ
Snijder, MB
Stallings, SC
Tanaka, T
Yanek, LR
Zhao, W
Becker, DM
Bielak, LF
Biggs, ML
Bottinger, EP
Bowden, DW
Chen, GJ
Correa, A
Couper, DJ
Crawford, DC
Cushman, M
Eicher, JD
Fornage, M
Franceschini, N
Fu, YP
Goodarzi, MO
Gottesman, O
Hara, K
Harris, TB
Jensen, RA
Johnson, AD
Jhun, MA
Karter, AJ
Keller, MF
Kho, AN
Kizer, JR
Krauss, RM
Langefeld, CD
Li, XH
Liang, JL
Liu, SM
Lowe, WL
Mosley, TH
North, KE
Pacheco, JA
Peyser, PA
Patrick, AL
Rice, KM
Selvin, E
Sims, M
Smith, JA
Tajuddin, SM
Vaidya, D
Wren, MP
Yao, J
Zhu, XF
Ziegler, JT
Zmuda, JM
Zonderman, AB
Zwinderman, AH
Adeyemo, A
Boerwinkle, E
Ferrucci, L
Hayes, MG
Kardia, SLR
Miljkovic, I
Pankow, JS
Rotimi, CN
Sale, MM
Wagenknecht, LE
Arnett, DK
Chen, YDI
Nalls, MA
Province, MA
Kao, WHL
Siscovick, DS
Psaty, BM
Wilson, JG
Loos, RJF
Dupuis, J
Rich, SS
Florez, JC
Rotter, JI
Morris, AP
Meigs, JB
AF Liu, Ching-Ti
Raghavan, Sridharan
Maruthur, Nisa
Kabagambe, Edmond Kato
Hong, Jaeyoung
Ng, Maggie C. Y.
Hivert, Marie-France
Lu, Yingchang
An, Ping
Bentley, Amy R.
Drolet, Anne M.
Gaulton, Kyle J.
Guo, Xiuqing
Armstrong, Loren L.
Irvin, Marguerite R.
Li, Man
Lipovich, Leonard
Rybin, Denis V.
Taylor, Kent D.
Agyemang, Charles
Palmer, Nicholette D.
Cade, Brian E.
Chen, Wei-Min
Dauriz, Marco
Delaney, Joseph A. C.
Edwards, Todd L.
Evans, Daniel S.
Evans, Michele K.
Lange, Leslie A.
Leong, Aaron
Liu, Jingmin
Liu, Yongmei
Nayak, Uma
Patel, Sanjay R.
Porneala, Bianca C.
Rasmussen-Torvik, Laura J.
Snijder, Marieke B.
Stallings, Sarah C.
Tanaka, Toshiko
Yanek, Lisa R.
Zhao, Wei
Becker, Diane M.
Bielak, Lawrence F.
Biggs, Mary L.
Bottinger, Erwin P.
Bowden, Donald W.
Chen, Guanjie
Correa, Adolfo
Couper, David J.
Crawford, Dana C.
Cushman, Mary
Eicher, John D.
Fornage, Myriam
Franceschini, Nora
Fu, Yi-Ping
Goodarzi, Mark O.
Gottesman, Omri
Hara, Kazuo
Harris, Tamara B.
Jensen, Richard A.
Johnson, Andrew D.
Jhun, Min A.
Karter, Andrew J.
Keller, Margaux F.
Kho, Abel N.
Kizer, Jorge R.
Krauss, Ronald M.
Langefeld, Carl D.
Li, Xiaohui
Liang, Jingling
Liu, Simin
Lowe, William L., Jr.
Mosley, Thomas H.
North, Kari E.
Pacheco, Jennifer A.
Peyser, Patricia A.
Patrick, Alan L.
Rice, Kenneth M.
Selvin, Elizabeth
Sims, Mario
Smith, Jennifer A.
Tajuddin, Salman M.
Vaidya, Dhananjay
Wren, Mary P.
Yao, Jie
Zhu, Xiaofeng
Ziegler, Julie T.
Zmuda, Joseph M.
Zonderman, Alan B.
Zwinderman, Aeilko H.
Adeyemo, Adebowale
Boerwinkle, Eric
Ferrucci, Luigi
Hayes, M. Geoffrey
Kardia, Sharon L. R.
Miljkovic, Iva
Pankow, James S.
Rotimi, Charles N.
Sale, Michele M.
Wagenknecht, Lynne E.
Arnett, Donna K.
Chen, Yii-Der Ida
Nalls, Michael A.
Province, Michael A.
Kao, W. H. Linda
Siscovick, David S.
Psaty, Bruce M.
Wilson, James G.
Loos, Ruth J. F.
Dupuis, Josee
Rich, Stephen S.
Florez, Jose C.
Rotter, Jerome I.
Morris, Andrew P.
Meigs, James B.
CA AAAG Consortium
CARe Consortium
COGENT-BP Consortium
eMERGE Consortium
MEDIA Consortium
MAGIC Consortium
TI Trans-ethnic Meta-analysis and Functional Annotation Illuminates the
Genetic Architecture of Fasting Glucose and Insulin
SO AMERICAN JOURNAL OF HUMAN GENETICS
LA English
DT Article
ID GENOME-WIDE ASSOCIATION; TYPE-2 DIABETES RISK; GLUCOKINASE REGULATORY
PROTEIN; BETA-CELL FUNCTION; BODY-MASS INDEX; TRIGLYCERIDE LEVELS;
PLASMA-GLUCOSE; TRANSETHNIC METAANALYSIS; AFRICAN-ANCESTRY; GLYCEMIC
TRAITS
AB Knowledge of the genetic basis of the type 2 diabetes (T2D)-related quantitative traits fasting glucose (FG) and insulin (FI) in African ancestry (AA) individuals has been limited. In non-diabetic subjects of AA (n = 20,209) and European ancestry (EA; n = 57,292), we performed trans-ethnic (AA+EA) fine-mapping of 54 established EA FG or FI loci with detailed functional annotation, assessed their relevance in AA individuals, and sought previously undescribed loci through trans-ethnic (AA+EA) meta-analysis. We narrowed credible sets of variants driving association signals for 22/54 EA-associated loci; 18/22 credible sets overlapped with active islet-specific enhancers or transcription factor (TF) binding sites, and 21/22 contained at least one TF motif. Of the 54 EA-associated loci, 23 were shared between EA and AA. Replication with an additional 10,096 AA individuals identified two previously undescribed FI loci, chrX FAM133A (rs213676) and chr5 PELO (rs6450057). Trans-ethnic analyses with regulatory annotation illuminate the genetic architecture of glycemic traits and suggest gene regulation as a target to advance precision medicine for T2D. Our approach to utilize state-of-the-art functional annotation and implement trans-ethnic association analysis for discovery and fine-mapping offers a framework for further follow-up and characterization of GWAS signals of complex trait loci.
C1 [Liu, Ching-Ti; Hong, Jaeyoung; Rybin, Denis V.; Dupuis, Josee] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA.
[Raghavan, Sridharan; Leong, Aaron; Porneala, Bianca C.; Meigs, James B.] Harvard Med Sch, Massachusetts Gen Hosp, Div Gen Internal Med, Boston, MA 02114 USA.
[Raghavan, Sridharan] Eastern Colorado Hlth Care Syst, Dept Vet Affairs Med Ctr, Denver, CO 80220 USA.
[Raghavan, Sridharan] Univ Colorado, Sch Med, Dept Med, Div Gen Internal Med, Denver, CO 80220 USA.
[Maruthur, Nisa] Johns Hopkins Univ, Sch Med, Div Gen Internal Med, Baltimore, MD 21287 USA.
[Maruthur, Nisa; Selvin, Elizabeth] Johns Hopkins Univ, Welch Ctr Prevent Epidemiol & Clin Res, Baltimore, MD 21287 USA.
[Maruthur, Nisa; Li, Man; Selvin, Elizabeth; Vaidya, Dhananjay; Kao, W. H. Linda] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21287 USA.
[Kabagambe, Edmond Kato; Edwards, Todd L.] Vanderbilt Univ, Med Ctr, Sch Med, Div Epidemiol,Dept Med, Nashville, TN 37203 USA.
[Ng, Maggie C. Y.; Palmer, Nicholette D.; Bowden, Donald W.] Wake Forest Univ, Sch Med, Ctr Genom & Personalized Med Res, Winston Salem, NC 27157 USA.
[Ng, Maggie C. Y.; Bowden, Donald W.] Wake Forest Univ, Bowman Gray Sch Med, Ctr Diabet Res, Winston Salem, NC 27157 USA.
[Hivert, Marie-France] Harvard Med Sch, Harvard Pilgrim Hlth Care Inst, Dept Populat Med, Boston, MA 02215 USA.
[Hivert, Marie-France; Florez, Jose C.] Massachusetts Gen Hosp, Diabet Unit, Boston, MA 02114 USA.
[Hivert, Marie-France] Univ Sherbrooke, Dept Med, Sherbrooke, PQ J1G 0A2, Canada.
[Lu, Yingchang; Bottinger, Erwin P.; Gottesman, Omri; Hara, Kazuo; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, New York, NY 10029 USA.
[Lu, Yingchang; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Genet Obes & Related Metab Traits Program, New York, NY 10029 USA.
[An, Ping; Province, Michael A.] Washington Univ, Sch Med, Dept Genet, Div Stat Genom, St Louis, MO 63108 USA.
[Bentley, Amy R.; Chen, Guanjie; Adeyemo, Adebowale; Rotimi, Charles N.] NHGRI, Ctr Res Genom & Global Hlth, NIH, Bethesda, MD 20892 USA.
[Drolet, Anne M.; Lipovich, Leonard] Wayne State Univ, Sch Med, Ctr Mol Med & Genet, Detroit, MI 48201 USA.
[Gaulton, Kyle J.; Morris, Andrew P.] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England.
[Guo, Xiuqing; Taylor, Kent D.; Li, Xiaohui; Yao, Jie; Chen, Yii-Der Ida; Rotter, Jerome I.] Harbor UCLA Med Ctr, Los Angeles Biomed Res Inst, Dept Pediat, Inst Translat Genom & Populat Sci, Torrance, CA 90502 USA.
[Armstrong, Loren L.; Kho, Abel N.; Lowe, William L., Jr.; Pacheco, Jennifer A.; Hayes, M. Geoffrey] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA.
[Irvin, Marguerite R.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA.
[Lipovich, Leonard] Wayne State Univ, Sch Med, Dept Neurol, Detroit, MI 48201 USA.
[Agyemang, Charles; Snijder, Marieke B.; Zwinderman, Aeilko H.] Acad Med Ctr Amsterdam, Dept Publ Hlth, Meibergdreef 15, NL-1105 AZ Amsterdam, Netherlands.
[Palmer, Nicholette D.; Bowden, Donald W.; Wren, Mary P.] Wake Forest Univ, Bowman Gray Sch Med, Dept Biochem, Winston Salem, NC 27157 USA.
[Cade, Brian E.] Harvard Med Sch, Brigham & Womens Hosp, Div Sleep & Circadian Disorders, Boston, MA 02115 USA.
[Chen, Wei-Min; Nayak, Uma; Sale, Michele M.; Rich, Stephen S.] Univ Virginia, Sch Med, Dept Publ Hlth Sci, Ctr Publ Hlth Genom, Charlottesville, VA 22908 USA.
[Dauriz, Marco] Univ Verona, Dept Med, Div Endocrinol Diabet & Metab, I-37126 Verona, Italy.
[Delaney, Joseph A. C.; Siscovick, David S.; Psaty, Bruce M.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA.
[Evans, Daniel S.] Calif Pacific Med Ctr, Res Inst, San Francisco, CA 94107 USA.
[Evans, Michele K.; Tajuddin, Salman M.] NIA, Hlth Dispar Res Sect, Lab Epidemiol & Populat Sci, NIH, Baltimore, MD 21224 USA.
[Lange, Leslie A.] Univ N Carolina, Dept Genet, Chapel Hill, NC 27607 USA.
[Liu, Jingmin] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA.
[Liu, Yongmei] Wake Forest Univ, Bowman Gray Sch Med, Div Publ Hlth Sci, Ctr Human Genet, Winston Salem, NC 27157 USA.
[Patel, Sanjay R.] Univ Pittsburgh, Dept Med, Pittsburgh, PA 15213 USA.
[Rasmussen-Torvik, Laura J.] Northwestern Univ, Feinberg Sch Med, Dept Prevent Med, Chicago, IL 60611 USA.
[Tanaka, Toshiko; Ferrucci, Luigi] NIA, Translat Gerontol Branch, Harbor Hosp, Baltimore, MD 21225 USA.
[Yanek, Lisa R.; Becker, Diane M.; Vaidya, Dhananjay] Johns Hopkins Univ, Dept Med, Div Gen Internal Med, GeneSTAR Res Program, Baltimore, MD 21287 USA.
[Zhao, Wei; Bielak, Lawrence F.; Jhun, Min A.; Peyser, Patricia A.; Smith, Jennifer A.; Kardia, Sharon L. R.] Univ Michigan, Sch Publ Hlth, Dept Epidemiol, Ann Arbor, MI 48109 USA.
[Becker, Diane M.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Hlth Policy & Management, Baltimore, MD 21287 USA.
[Biggs, Mary L.; Rice, Kenneth M.] Univ Washington, Dept Biostat, Seattle, WA 98195 USA.
[Biggs, Mary L.; Jensen, Richard A.; Siscovick, David S.; Psaty, Bruce M.] Univ Washington, Sch Med, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA 98195 USA.
[Correa, Adolfo; Sims, Mario] Univ Mississippi, Med Ctr, Dept Med, Jackson, MS 39216 USA.
[Couper, David J.] Univ N Carolina, Gillings Sch Global Publ Hlth, Dept Biostat, Collaborat Studies Coordinating Ctr, Chapel Hill, NC 27514 USA.
[Crawford, Dana C.] Case Western Reserve Univ, Inst Computat Biol, Dept Epidemiol & Biostat, Cleveland, OH 44106 USA.
[Cushman, Mary] Univ Vermont, Coll Med, Dept Pathol & Med, Burlington, VT 05405 USA.
[Eicher, John D.; Dupuis, Josee] NHLBI, Framingham Heart Study, Framingham, MA 01702 USA.
[Eicher, John D.; Johnson, Andrew D.] NHLBI, Populat Sci Branch, Div Intramural Res, NIH, Framingham, MA 01702 USA.
[Fornage, Myriam; Boerwinkle, Eric] Univ Texas Hlth Sci Ctr Houston, Inst Mol Med, Houston, TX 77030 USA.
[Fornage, Myriam; Boerwinkle, Eric] Univ Texas Hlth Sci Ctr Houston, Ctr Human Genet, Houston, TX 77030 USA.
[Franceschini, Nora; North, Kari E.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27514 USA.
[Fu, Yi-Ping] NHLBI, Cardiovasc Epidemiol & Human Genom Branch, NIH, Framingham, MA 01702 USA.
[Goodarzi, Mark O.] Cedars Sinai Med Ctr, Div Endocrinol Diabet & Metab, Los Angeles, CA 90048 USA.
[Hara, Kazuo] Univ Tokyo, Grad Sch Med, Dept Diabet & Metab Dis, Tokyo 1138655, Japan.
[Hara, Kazuo] Tokyo Med Univ, Dept Diabet Endocrinol & Metab, Tokyo 1630023, Japan.
[Harris, Tamara B.] NIH, Lab Epidemiol & Populat Sci, Bethesda, MD 20892 USA.
[Karter, Andrew J.] Kaiser Permanente, Div Res, Oakland, CA 94612 USA.
[Keller, Margaux F.] Merck Res Labs, Dept Genet & Pharmacogen, 33 Ave Louis Pasteur, Boston, MA 02115 USA.
[Kizer, Jorge R.] Montefiore Med Ctr, Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA.
[Kizer, Jorge R.] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10461 USA.
[Krauss, Ronald M.] Childrens Hosp Oakland, Res Inst, Oakland, CA 94609 USA.
[Langefeld, Carl D.; Ziegler, Julie T.] Wake Forest Univ, Bowman Gray Sch Med, Ctr Publ Hlth Genom, Winston Salem, NC 27157 USA.
[Langefeld, Carl D.; Ziegler, Julie T.] Wake Forest Univ, Bowman Gray Sch Med, Dept Biostat Sci, Winston Salem, NC 27157 USA.
[Liang, Jingling; Zhu, Xiaofeng] Case Western Reserve Univ, Dept Epidemiol & Biostat, Cleveland, OH 44106 USA.
[Liu, Simin] Brown Univ, Dept Epidemiol, Providence, RI 02912 USA.
[Liu, Simin] Brown Univ, Dept Med, Providence, RI 02903 USA.
[Mosley, Thomas H.] Univ Mississippi, Med Ctr, Dept Med, Div Geriatr Gerontol, Jackson, MS 39216 USA.
[Patrick, Alan L.] Tobago Hlth Studies Off, Scarborough, Trinid & Tobago.
[Zmuda, Joseph M.; Miljkovic, Iva] Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15213 USA.
[Zonderman, Alan B.] NIA, Behav Epidemiol Sect, Lab Epidemiol & Populat Sci, Intramural Res Program,NIH, Baltimore, MD 21224 USA.
[Pankow, James S.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55455 USA.
[Wagenknecht, Lynne E.] Wake Forest Univ, Bowman Gray Sch Med, Div Publ Hlth Sci, Winston Salem, NC 27157 USA.
[Arnett, Donna K.] Univ Kentucky, Coll Publ Hlth, Lexington, KY 40563 USA.
[Nalls, Michael A.] NIA, Neurogenet Lab, NIH, Bethesda, MD 20892 USA.
[Siscovick, David S.] New York Acad Med, New York, NY 10029 USA.
[Psaty, Bruce M.] Univ Washington, Dept Hlth Serv, Seattle, WA 98195 USA.
[Psaty, Bruce M.] Grp Hlth Cooperat Puget Sound, Grp Hlth Res Inst, Seattle, WA 98101 USA.
[Wilson, James G.] Univ Mississippi, Med Ctr, Dept Physiol & Biophys, Jackson, MS 39216 USA.
[Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, New York, NY 10029 USA.
[Florez, Jose C.] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA.
[Florez, Jose C.] Broad Inst, Program Metab, Cambridge, MA 02142 USA.
[Florez, Jose C.] Broad Inst, Program Med & Populat Genet, Cambridge, MA 02142 USA.
[Florez, Jose C.] Harvard Med Sch, Dept Med, Boston, MA 02115 USA.
[Morris, Andrew P.] Univ Liverpool, Dept Biostat, Inst Translat Med, Liverpool L69 3BX, Merseyside, England.
[Stallings, Sarah C.] Vanderbilt Univ, Med Ctr, Vanderbilt Inst Clin & Translat Res, Nashville, TN 37203 USA.
RP Liu, CT (reprint author), Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA.; Meigs, JB (reprint author), Harvard Med Sch, Massachusetts Gen Hosp, Div Gen Internal Med, Boston, MA 02114 USA.
EM ctliu@bu.edu; jmeigs@partners.org
RI Johnson, Andrew/G-6520-2013; Dauriz, Marco/S-5843-2016;
OI Dauriz, Marco/0000-0002-5542-5941; Tajuddin, Salman
M./0000-0002-7919-8528; Patel, Sanjay/0000-0002-9142-5172; Zonderman,
Alan B/0000-0002-6523-4778; Vaidya, Dhananjay/0000-0002-7164-1601;
Smith, Jennifer/0000-0002-3575-5468; Pankow, James/0000-0001-7076-483X
FU Johnson Johnson
FX B.M.P. serves on the DSMB of a clinical trial for the device
manufacturer Zoll LifeCor and on the Steering Committee for the Yale
Open Data Access Project funded by Johnson & Johnson. D.V. is a
consultant for Consumable Science, Inc. J.R.K. holds stock in Pfizer,
Inc., and Gilead Sciences, Inc.
NR 61
TC 1
Z9 1
U1 1
U2 2
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0002-9297
EI 1537-6605
J9 AM J HUM GENET
JI Am. J. Hum. Genet.
PD JUL 7
PY 2016
VL 99
IS 1
BP 56
EP 75
DI 10.1016/j.ajhg.2016.05.006
PG 20
WC Genetics & Heredity
SC Genetics & Heredity
GA DT6TI
UT WOS:000381616600005
PM 27321945
ER
PT J
AU Yu, P
Yang, WL
Han, D
Wang, X
Guo, S
Li, JC
Li, F
Zhang, XX
Wong, SW
Bai, BJ
Liu, Y
Du, J
Sun, ZS
Shi, ST
Feng, HL
Cai, T
AF Yu, Ping
Yang, Wenli
Han, Dong
Wang, Xi
Guo, Sen
Li, Jinchen
Li, Fang
Zhang, Xiaoxia
Wong, Sing-Wai
Bai, Baojing
Liu, Yao
Du, Jie
Sun, Zhong Sheng
Shi, Songtao
Feng, Hailan
Cai, Tao
TI Mutations in WNT10B Are Identified in Individuals with Oligodontia
SO AMERICAN JOURNAL OF HUMAN GENETICS
LA English
DT Article
ID EPITHELIAL-MESENCHYMAL INTERACTIONS; AUTOSOMAL-DOMINANT OLIGODONTIA;
IN-VITRO; TOOTH; EXPRESSION; GENE; DIFFERENTIATION; DISEASE;
MORPHOGENESIS; POPULATION
AB Tooth agenesis is one of the most common developmental anomalies in humans. Oligodontia, a severe form of tooth agenesis, is genetically and phenotypically a heterogeneous condition. Although significant efforts have been made, the genetic etiology of dental agenesis remains largely unknown. In the present study, we performed whole-exome sequencing to identify the causative mutations in Chinese families in whom oligodontia segregates with dominant inheritance. We detected a heterozygous missense mutation (c.632G > A [p.Arg211Gln]) in WNT10B in all affected family members. By Sanger sequencing a cohort of 145 unrelated individuals with non-syndromic oligodontia, we identified three additional mutations (c.569C > G [p.Pro190Arg], c.786G > A [p.Trp262*], and c.851T > G [p.Phe284Cys]). Interestingly, analysis of genotype-phenotype correlations revealed that mutations in WNT10B affect the development of permanent dentition, particularly the lateral incisors. Furthermore, a functional assay demonstrated that each of these mutants could not normally enhance the canonical Wnt signaling in HEPG2 epithelial cells, in which activity of the TOPFlash luciferase reporter was measured. Notably, these mutant WNT10B ligands could not efficiently induce endothelial differentiation of dental pulp stem cells. Our findings provide the identification of autosomal-dominant WNT10B mutations in individuals with oligodontia, which increases the spectrum of congenital tooth agenesis and suggests attenuated Wnt signaling in endothelial differentiation of dental pulp stem cells.
C1 [Yu, Ping; Guo, Sen; Li, Jinchen; Cai, Tao] Wenzhou Med Univ, Inst Genom Med, Wenzhou 325000, Peoples R China.
[Yang, Wenli; Wang, Xi] Zhengzhou Univ, Stomatol Hosp, Zhengzhou 450052, Peoples R China.
[Han, Dong; Li, Fang; Zhang, Xiaoxia; Wong, Sing-Wai; Feng, Hailan] Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China.
[Wong, Sing-Wai] Univ North Carolina Chapel Hill, Sch Dent, Oral Biol Curriculum, Chapel Hill, NC 27599 USA.
[Bai, Baojing] Beijing Stomatol Hosp, Dept Prosthodont, Beijing 100050, Peoples R China.
[Liu, Yao; Shi, Songtao] Univ Penn, Sch Dent Med, Dept Anat & Cell Biol, Philadelphia, PA 19104 USA.
[Du, Jie] Capital Med Univ, Beijing Anzhen Hosp, Beijing 100000, Peoples R China.
[Sun, Zhong Sheng] Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100000, Peoples R China.
[Cai, Tao] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20982 USA.
RP Cai, T (reprint author), Wenzhou Med Univ, Inst Genom Med, Wenzhou 325000, Peoples R China.; Feng, HL (reprint author), Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China.; Cai, T (reprint author), Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD 20982 USA.
EM kqfenghl@bjmu.edu.cn; tcai@mail.nih.gov
FU Wenzhou Medical University Research Fund; Beijing Collaborative
Innovative Research Center for Cardiovascular Diseases
[PXM2014_014226_000002]; National Natural Science Foundation of China
[81110114]
FX We are indebted to the individuals with tooth agenesis and their
families for their participation in this study. We thank Dr. Curtis
Harris's lab for the wild-type WNT10B plasmid, Dr. DeeDee Smart's lab
for the pTOPFLASH and pCMV beta-gal plasmids, and Qianzhi Shao for
bioinformatics analysis. We wish to thank Drs. Yan Jin and Weidong Li
for thoughtful suggestions. Research reported in this publication was
supported by the Wenzhou Medical University Research Fund (to P.Y.), the
Beijing Collaborative Innovative Research Center for Cardiovascular
Diseases (PXM2014_014226_000002 to D.J.), and National Natural Science
Foundation of China grant 81110114 (to Z.S.S.).
NR 38
TC 0
Z9 0
U1 3
U2 3
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0002-9297
EI 1537-6605
J9 AM J HUM GENET
JI Am. J. Hum. Genet.
PD JUL 7
PY 2016
VL 99
IS 1
BP 195
EP 201
DI 10.1016/j.ajhg.2016.05.012
PG 7
WC Genetics & Heredity
SC Genetics & Heredity
GA DT6TI
UT WOS:000381616600016
PM 27321946
ER
PT J
AU McKiernan, EC
Bourne, PE
Brown, CT
Buck, S
Kenall, A
Lin, J
McDougall, D
Nosek, BA
Ram, K
Soderberg, CK
Spies, JR
Thaney, K
Updegrove, A
Woo, KH
Yarkoni, T
AF McKiernan, Erin C.
Bourne, Philip E.
Brown, C. Titus
Buck, Stuart
Kenall, Amye
Lin, Jennifer
McDougall, Damon
Nosek, Brian A.
Ram, Karthik
Soderberg, Courtney K.
Spies, Jeffrey R.
Thaney, Kaitlin
Updegrove, Andrew
Woo, Kara H.
Yarkoni, Tal
TI How open science helps researchers succeed
SO ELIFE
LA English
DT Article
ID RANDOMIZED CONTROLLED-TRIAL; OPEN-ACCESS ARTICLES; CITATION ADVANTAGE;
SCHOLARLY COMMUNICATION; SUBSCRIPTION JOURNALS; RESEARCH IMPACT;
PUBLICATIONS; DOWNLOADS; REVIEWERS; SYSTEM
AB Open access, open data, open source and other open scholarship practices are growing in popularity and necessity. However, widespread adoption of these practices has not yet been achieved. One reason is that researchers are uncertain about how sharing their work will affect their careers. We review literature demonstrating that open research is associated with increases in citations, media attention, potential collaborators, job opportunities and funding opportunities. These findings are evidence that open research practices bring significant benefits to researchers relative to more traditional closed practices.
C1 [McKiernan, Erin C.] Univ Nacl Autonoma Mexico, Fac Sci, Dept Phys, Mexico City, DF, Mexico.
[Bourne, Philip E.] NIH, Bethesda, MD 20892 USA.
[Brown, C. Titus] Univ Calif Davis, Populat Hlth & Reprod, Davis, CA 95616 USA.
[Buck, Stuart] Laura & John Arnold Fdn, Houston, TX USA.
[Kenall, Amye] BioMed Cent, London, England.
[Lin, Jennifer] CrossRef, Oxford, England.
[McDougall, Damon] Univ Texas Austin, Inst Computat Engn & Sci, Austin, TX 78712 USA.
[Nosek, Brian A.; Soderberg, Courtney K.; Spies, Jeffrey R.] Ctr Open Sci, Charlottesville, VA USA.
[Ram, Karthik] Univ Calif Berkeley, Berkeley Inst Data Sci, Berkeley, CA 94720 USA.
[Spies, Jeffrey R.] Univ Virginia, Dept Engn & Soc, Charlottesville, VA USA.
[Thaney, Kaitlin] Mozilla Fdn, Mozilla Sci Lab0, New York, NY USA.
[Updegrove, Andrew] Gesmer Updegrove LLP, Boston, MA USA.
[Woo, Kara H.] Washington State Univ, Ctr Environm Res Educ & Outreach, Pullman, WA USA.
[Woo, Kara H.] Univ Washington, Informat Sch, Seattle, WA 98195 USA.
[Yarkoni, Tal] Univ Texas Austin, Dept Psychol, Austin, TX 78712 USA.
RP McKiernan, EC (reprint author), Univ Nacl Autonoma Mexico, Fac Sci, Dept Phys, Mexico City, DF, Mexico.
EM emckiernan@ciencias.unam.mx
OI McKiernan, Erin/0000-0002-9430-5221; Lin, Jennifer/0000-0002-9680-2328;
Woo, Kara/0000-0002-5125-4188; Bourne, Philip/0000-0002-7618-7292;
Kenall, Amye/0000-0002-3030-8001
NR 140
TC 7
Z9 7
U1 24
U2 25
PU ELIFE SCIENCES PUBLICATIONS LTD
PI CAMBRIDGE
PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND
SN 2050-084X
J9 ELIFE
JI eLife
PD JUL 7
PY 2016
VL 5
AR e16800
DI 10.7554/eLife.16800
PG 19
WC Biology
SC Life Sciences & Biomedicine - Other Topics
GA DV2HA
UT WOS:000382740200001
ER
PT J
AU Chami, N
Chen, MH
Slater, AJ
Eicher, JD
Evangelou, E
Tajuddin, SM
Love-Gregory, L
Kacprowski, T
Schick, UM
Nomura, A
Giri, A
Lessard, S
Brody, JA
Schurmann, C
Pankratz, N
Yanek, LR
Manichaikul, A
Pazoki, R
Mihailov, E
Hill, WD
Raffield, LM
Burt, A
Bartz, TM
Becker, DM
Becker, LC
Boerwinkle, E
Bork-Jensen, J
Bottinger, EP
O'Donoghue, ML
Crosslin, DR
de Denus, S
Dube, MP
Elliott, P
Engstrom, G
Evans, MK
Floyd, JS
Fornage, M
Gao, H
Greinacher, A
Gudnason, V
Hansen, T
Harris, TB
Hayward, C
Hernesniemi, J
Highland, HM
Hirschhorn, JN
Hofman, A
Irvin, MR
Kahonen, M
Lange, E
Launer, LJ
Lehtimaki, T
Li, J
Liewald, DCM
Linneberg, A
Liu, YM
Lu, YC
Lyytikainen, LP
Magi, R
Mathias, RA
Melander, O
Metspalu, A
Mononen, N
Nalls, MA
Nickerson, DA
Nikus, K
O'Donnell, CJ
Orho-Melander, M
Pedersen, O
Petersmann, A
Polfus, L
Psaty, BM
Raitakari, OT
Raitoharju, E
Richard, M
Rice, KM
Rivadeneira, F
Rotter, JI
Schmidt, F
Smith, AV
Starr, JM
Taylor, KD
Teumer, A
Thuesen, BH
Torstenson, ES
Tracy, RP
Tzoulaki, I
Zakai, NA
Vacchi-Suzzi, C
van Duijn, CM
van Rooij, FJA
Cushman, M
Deary, IJ
Edwards, DRV
Vergnaud, AC
Wallentin, L
Waterworth, DM
White, HD
Wilson, JG
Zonderman, AB
Kathiresan, S
Grarup, N
Esko, T
Loos, RJF
Lange, LA
Faraday, N
Abumrad, NA
Edwards, TL
Ganesh, SK
Auer, PL
Johnson, AD
Reiner, AP
Lettre, G
AF Chami, Nathalie
Chen, Ming-Huei
Slater, Andrew J.
Eicher, John D.
Evangelou, Evangelos
Tajuddin, Salman M.
Love-Gregory, Latisha
Kacprowski, Tim
Schick, Ursula M.
Nomura, Akihiro
Giri, Ayush
Lessard, Samuel
Brody, Jennifer A.
Schurmann, Claudia
Pankratz, Nathan
Yanek, Lisa R.
Manichaikul, Ani
Pazoki, Raha
Mihailov, Evelin
Hill, W. David
Raffield, Laura M.
Burt, Amber
Bartz, Traci M.
Becker, Diane M.
Becker, Lewis C.
Boerwinkle, Eric
Bork-Jensen, Jette
Bottinger, Erwin P.
O'Donoghue, Michelle L.
Crosslin, David R.
de Denus, Simon
Dube, Marie-Pierre
Elliott, Paul
Engstrom, Gunnar
Evans, Michele K.
Floyd, James S.
Fornage, Myriam
Gao, He
Greinacher, Andreas
Gudnason, Vilmundur
Hansen, Torben
Harris, Tamara B.
Hayward, Caroline
Hernesniemi, Jussi
Highland, Heather M.
Hirschhorn, Joel N.
Hofman, Albert
Irvin, Marguerite R.
Kahonen, Mika
Lange, Ethan
Launer, Lenore J.
Lehtimaki, Terho
Li, Jin
Liewald, David C. M.
Linneberg, Allan
Liu, Yongmei
Lu, Yingchang
Lyytikainen, Leo-Pekka
Magi, Reedik
Mathias, Rasika A.
Melander, Olle
Metspalu, Andres
Mononen, Nina
Nalls, Mike A.
Nickerson, Deborah A.
Nikus, Kjell
O'Donnell, Chris J.
Orho-Melander, Marju
Pedersen, Oluf
Petersmann, Astrid
Polfus, Linda
Psaty, Bruce M.
Raitakari, Olli T.
Raitoharju, Emma
Richard, Melissa
Rice, Kenneth M.
Rivadeneira, Fernando
Rotter, Jerome I.
Schmidt, Frank
Smith, Albert Vernon
Starr, John M.
Taylor, Kent D.
Teumer, Alexander
Thuesen, Betina H.
Torstenson, Eric S.
Tracy, Russell P.
Tzoulaki, Ioanna
Zakai, Neil A.
Vacchi-Suzzi, Caterina
van Duijn, Cornelia M.
van Rooij, Frank J. A.
Cushman, Mary
Deary, Ian J.
Edwards, Digna R. Velez
Vergnaud, Anne-Claire
Wallentin, Lars
Waterworth, Dawn M.
White, Harvey D.
Wilson, James G.
Zonderman, Alan B.
Kathiresan, Sekar
Grarup, Niels
Esko, Tonu
Loos, Ruth J. F.
Lange, Leslie A.
Faraday, Nauder
Abumrad, Nada A.
Edwards, Todd L.
Ganesh, Santhi K.
Auer, Paul L.
Johnson, Andrew D.
Reiner, Alexander P.
Lettre, Guillaume
TI Exome Genotyping Identifies Pleiotropic Variants Associated with Red
Blood Cell Traits
SO AMERICAN JOURNAL OF HUMAN GENETICS
LA English
DT Article
ID GENOME-WIDE ASSOCIATION; CORONARY-HEART-DISEASE; AFRICAN-AMERICANS;
DILATED CARDIOMYOPATHY; NATURAL-SELECTION; SEQUENCE VARIANTS;
PYRUVATE-KINASE; LOW-FREQUENCY; LOCI; GENE
AB Red blood cell (RBC) traits are important heritable clinical biomarkers and modifiers of disease severity. To identify coding genetic variants associated with these traits, we conducted meta-analyses of seven RBC phenotypes in 130,273 multi-ethnic individuals from studies genotyped on an exome array. After conditional analyses and replication in 27,480 independent individuals, we identified 16 new RBC variants. We found low-frequency missense variants in MAP1A (rs55707100, minor allele frequency [MAF] = 3.3%, p = 2 x 10(-10) for hemoglobin [HGB]) and HNF4A (rs1800961, MAF = 2.4%, p < 3 x 10(-8) for hematocrit [HCT] and HGB). In African Americans, we identified a nonsense variant in CD36 associated with higher RBC distribution width (rs3211938, MAF = 8.7%, p = 7 x 10(-11)) and showed that it is associated with lower CD36 expression and strong allelic imbalance in ex vivo differentiated human erythroblasts. We also identified a rare missense variant in ALAS2 (rs201062903, MAF = 0.2%) associated with lower mean corpuscular volume and mean corpuscular hemoglobin (p < 8 x 10(-9)). Mendelian mutations in ALAS2 are a cause of sideroblastic anemia and erythropoietic protoporphyria. Gene-based testing highlighted three rare missense variants in PKLR, a gene mutated in Mendelian non-spherocytic hemolytic anemia, associated with HGB and HCT (SKAT p < 8 x 10(-7)). These rare, low-frequency, and common RBC variants showed pleiotropy, being also associated with platelet, white blood cell, and lipid traits. Our association results and functional annotation suggest the involvement of new genes in human erythropoiesis. We also confirm that rare and low-frequency variants play a role in the architecture of complex human traits, although their phenotypic effect is generally smaller than originally anticipated.
C1 [Chami, Nathalie; Lessard, Samuel; Dube, Marie-Pierre; Lettre, Guillaume] Univ Montreal, Dept Med, Montreal, PQ H3T 1J4, Canada.
[Chami, Nathalie; Lessard, Samuel; de Denus, Simon; Dube, Marie-Pierre; Lettre, Guillaume] Montreal Heart Inst, Montreal, PQ H1T 1CB, Canada.
[Chen, Ming-Huei; Eicher, John D.; O'Donnell, Chris J.; Johnson, Andrew D.] NHLBI, Populat Sci Branch, Framingham Heart Study, Framingham, MA 01702 USA.
[Slater, Andrew J.] GlaxoSmithKline, Genet Target Sci, Res Triangle Pk, Res Triangle Pk, NC 27709 USA.
[Slater, Andrew J.] OmicSoft Corp, Cary, NC 27513 USA.
[Evangelou, Evangelos; Elliott, Paul; Gao, He; Tzoulaki, Ioanna; Vergnaud, Anne-Claire] Imperial Coll London, Dept Epidemiol & Biostat, MRC PHE Ctr Environm & Hlth, Sch Publ Hlth, London W2 1PG, England.
[Evangelou, Evangelos; Tzoulaki, Ioanna] Univ Ioannina, Sch Med, Dept Hyg & Epidemiol, Ioannina 45110, Greece.
[Tajuddin, Salman M.; Evans, Michele K.; Zonderman, Alan B.] NIA, Lab Epidemiol & Populat Sci, Baltimore, MD 21224 USA.
[Love-Gregory, Latisha; Abumrad, Nada A.] Washington Univ, Sch Med, Dept Med, Ctr Human Nutr, St Louis, MO 63110 USA.
[Kacprowski, Tim; Schmidt, Frank] Ernst Moritz Arndt Univ Greifswald, Dept Funct Gen, Interfaculty Inst Genet & Funct Gen, Univ Med, D-17475 Greifswald, Germany.
[Kacprowski, Tim] DZHK German Ctr Cardiovasc Res, Partner Site Greifswald, Greifswald, Germany.
[Schick, Ursula M.; Schurmann, Claudia; Bottinger, Erwin P.; Lu, Yingchang; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, New York, NY 10069 USA.
[Nomura, Akihiro; Kathiresan, Sekar] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA.
[Nomura, Akihiro; Hirschhorn, Joel N.; Kathiresan, Sekar; Esko, Tonu] Broad Inst, Program Med & Populat Genet, Cambridge, MA 02142 USA.
[Nomura, Akihiro; Kathiresan, Sekar] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA.
[Nomura, Akihiro; Kathiresan, Sekar] Harvard Med Sch, Dept Med, Boston, MA 02115 USA.
[Nomura, Akihiro] Kanazawa Univ, Grad Sch Med Sci, Div Cardiovasc Med, Kanazawa, Ishikawa 9200942, Japan.
[Giri, Ayush; Torstenson, Eric S.; Edwards, Todd L.] Vanderbilt Univ, Inst Med & Publ Hlth, Vanderbilt Genet Inst, Div Epidemiol, Nashville, TN 37235 USA.
[Brody, Jennifer A.; Floyd, James S.] Univ Washington, Dept Med, Seattle, WA 98101 USA.
[Schurmann, Claudia; Lu, Yingchang; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Genet Obes & Related Metab Traits Program, New York, NY 10069 USA.
[Pankratz, Nathan] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55454 USA.
[Yanek, Lisa R.; Becker, Diane M.] Johns Hopkins Univ, Sch Med, Dept Med Div Gen Internal Med icine, Div Gen Internal Med, Baltimore, MD 21205 USA.
[Manichaikul, Ani] Univ Virginia, Ctr Publ Hlth Gen, Charlottesville, VA 22908 USA.
[Pazoki, Raha; Hofman, Albert; Rivadeneira, Fernando; van Duijn, Cornelia M.; van Rooij, Frank J. A.] Erasmus, Dept Epidemiol, NL-3000 Mcrotterdam, Netherlands.
[Mihailov, Evelin; Magi, Reedik; Metspalu, Andres; Esko, Tonu] Univ Tartu, Estonian Genome Ctr, EE-51010 Tartu, Estonia.
[Hill, W. David; Liewald, David C. M.; Starr, John M.; Deary, Ian J.] Univ Edinburgh, Ctr Cognit Ageing & Cognit Epidemiol, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Hill, W. David; Liewald, David C. M.; Deary, Ian J.] Univ Edinburgh, Dept Psychol, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Raffield, Laura M.; Lange, Leslie A.] Univ N Carolina, Dept Genet, Chapel Hill, NC 27514 USA.
[Burt, Amber] Univ Washington, Div Med Genet, Dept Med, Seattle, WA 98195 USA.
[Bartz, Traci M.; Rice, Kenneth M.] Univ Washington, Dept Biostat, Seattle, WA 98195 USA.
[Becker, Lewis C.] Johns Hopkins Univ Sch Med, Dept Med, Div Cardiol & Gen Internal Med, Baltimore, MD 21205 USA.
[Boerwinkle, Eric; Highland, Heather M.; Polfus, Linda] Univ Texas Hlth Sci Ctr, Sch Publ Hlth, Ctr Human Genet, Houston, TX 77030 USA.
[Boerwinkle, Eric] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA.
[Bork-Jensen, Jette; Hansen, Torben; Pedersen, Oluf; Grarup, Niels] Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn, Ctr Basic Metabol Res, DK-2100 Copenhagen, Denmark.
[O'Donoghue, Michelle L.] Brigham & Womens Hosp, Cardiovasc Div, TIMI Study Grp, Boston, MA 02115 USA.
[Crosslin, David R.] Univ Washington, Dept Biomed Informat & Med Educ, Seattle, WA 98195 USA.
[de Denus, Simon] Univ Montreal, Fac Pharm, Montreal, PQ H3T 1J4, Canada.
[Engstrom, Gunnar; Melander, Olle; Orho-Melander, Marju] Lund Univ, Dept Clin Sci, S-22100 Malmo, Sweden.
[Engstrom, Gunnar; Melander, Olle; Orho-Melander, Marju] Skane Univ Hosp, S-22241 Malmo, Sweden.
[Fornage, Myriam; Richard, Melissa] Univ Texas Hlth Sci Ctr, Inst Mol Med, Houston, TX 77030 USA.
[Greinacher, Andreas] Univ Med Greifswald, Inst Immunol & Transfus Med, D-17475 Greifswald, Germany.
[Gudnason, Vilmundur; Smith, Albert Vernon] Iceland Heart Assoc, IS-201 Kopavogur, Iceland.
[Gudnason, Vilmundur; Smith, Albert Vernon] Univ Iceland, Fac Med, IS-101 Reykjavik, Iceland.
[Harris, Tamara B.; Launer, Lenore J.] NIA, Intramural Res Program, NIH, Lab Epidemiol Demog & Biometry, Bethesda, MD 20892 USA.
[Hayward, Caroline] Univ Edinburgh, MRC Human Genet Unit, Inst Genet & Mol Med, Edinburgh EH4 2XU, Midlothian, Scotland.
[Hernesniemi, Jussi; Lehtimaki, Terho; Lyytikainen, Leo-Pekka; Mononen, Nina; Raitoharju, Emma] Fimlab Labs, Dept Clin Chem, Tampere 33520, Finland.
[Hernesniemi, Jussi; Lehtimaki, Terho; Lyytikainen, Leo-Pekka; Mononen, Nina; Raitoharju, Emma] Univ Tampere, Dept Clin Chem, Sch Med, Tampere 33014, Finland.
[Hernesniemi, Jussi; Nikus, Kjell] Univ Tampere, Sch Med, Tampere 33014, Finland.
[Highland, Heather M.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27514 USA.
[Hirschhorn, Joel N.] Boston Childrens Hosp, Dept Endocrinol, Boston, MA 02115 USA.
[Hofman, Albert] Harvard TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA.
[Irvin, Marguerite R.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35233 USA.
[Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere 33521, Finland.
[Kahonen, Mika] Univ Tampere, Dept Clin Physiol, Sch Med, Tampere 33014, Finland.
[Lange, Ethan] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA.
[Lange, Ethan] Univ N Carolina, Dept Biostat, Chapel Hill, NC 27599 USA.
[Li, Jin] Stanford Univ, Sch Med, Div Cardiovasc Med, Dept Med, Palo Alto, CA 94305 USA.
[Linneberg, Allan; Thuesen, Betina H.] Capital Region Denmark, Res Ctr Prevent & Hlth, DK-2600 Copenhagen, Denmark.
[Linneberg, Allan] Rigshosp, Dept Clin Expt Res, DK-2100 Glostrup, Denmark.
[Linneberg, Allan] Univ Copenhagen, Fac Hlth & Med Sci, Dept Clin Med, DK-2200 Copenhagen, Denmark.
[Liu, Yongmei] Wake Forest Sch Med, Div Publ Hlth Sci, Ctr Human Genet, Winston Salem, NC 27157 USA.
[Mathias, Rasika A.] Johns Hopkins Univ Sch Med, Dept Med, Div Allergy & Clin Immunol, Baltimore, MD 21205 USA.
[Mathias, Rasika A.] Johns Hopkins Univ Sch Med, Div Gen Internal Med, Baltimore, MD 21205 USA.
[Nalls, Mike A.] NIA, NIH, Neurogenet Lab, Bethesda, MD 20892 USA.
[Nickerson, Deborah A.] Univ Washington, Dept Genome Sci, Seattle, WA 98105 USA.
[Nikus, Kjell] Tampere Univ Hosp, Dept Cardiol, Ctr Heart, Tampere 33521, Finland.
[O'Donnell, Chris J.] Boston Vet Adm VA Healthcare, Cardiol Sect, Boston, MA 02118 USA.
[O'Donnell, Chris J.] Boston Vet Adm VA Healthcare, Ctr Populat Gen, Boston, MA 02118 USA.
[Petersmann, Astrid] Univ Med Greifswald, Inst Clin Chem & Lab Med, D-17475 Greifswald, Germany.
[Psaty, Bruce M.] Univ Washington, Dept Med Epidemiol & Hlth Serv, Cardiovasc Hlth Res Unit, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Grp Hlth Res Inst, Grp Hlth Cooperat, Seattle, WA 98101 USA.
[Raitakari, Olli T.] Turku Univ Hosp, Dept Clin Physiol & Nucl Med, Turku 20521, Finland.
[Raitakari, Olli T.] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku 20520, Finland.
[Rivadeneira, Fernando] Erasmus MC, Dept Internal Med, NL-3000 Rotterdam, Netherlands.
[Rivadeneira, Fernando] NCHA, NL-3015 Rotterdam, Netherlands.
[Rotter, Jerome I.; Taylor, Kent D.] Los Angeles Biomed Res Inst, Inst Translat Genom & Populat Sci, Torrance, CA 90502 USA.
[Rotter, Jerome I.; Taylor, Kent D.] Harbor UCLA Med Ctr, Dept Pediat, Torrance, CA 90502 USA.
[Starr, John M.] Alzheimer Scotland Res Ctr, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Teumer, Alexander] Univ Med Greifswald, Inst Community Med, D-17475 Greifswald, Germany.
[Tracy, Russell P.] Univ Vermont Coll Med, Dept Pathol, Colchester, VT 05446 USA.
[Tracy, Russell P.] Univ Vermont Coll Med, Dept Lab Med, Colchester, VT 05446 USA.
[Tracy, Russell P.] Univ Vermont Coll Med, Dept Biochem, Colchester, VT 05446 USA.
[Zakai, Neil A.; Cushman, Mary] Univ Vermont Coll Med, Dept Med, Burlington, VT 05405 USA.
[Zakai, Neil A.; Cushman, Mary] Univ Vermont Coll Med, Dept Pathol, Burlington, VT 05405 USA.
[Vacchi-Suzzi, Caterina] SUNY Stony Brook, Dept Family Populat & Prevent Med, Stony Brook, NY 11794 USA.
[Edwards, Digna R. Velez] Vanderbilt Univ, Vanderbilt Epidemiol Ctr, Dept Obstet & Gynecol, Inst Med & Publ Hlth,Vanderbilt Genet Inst, Nashville, TN 37203 USA.
[Wallentin, Lars] Uppsala Univ, Dept Med Sci, Cardiol & Uppsala Clin Res Ctr, S-75185 Uppsala, Sweden.
[Waterworth, Dawn M.] Genet Target Sci, GlaxoSmithKline, King Of Prussia, PA 19406 USA.
[White, Harvey D.] Auckland City Hosp, Green Lane Cardiovasc Serv, Auckland 1142, New Zealand.
[White, Harvey D.] Univ Auckland, Auckland 1142, New Zealand.
[Wilson, James G.] Univ Mississippi Med Ctr, Dept Physiol & Biophys, Jackson, MS 39216 USA.
[Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, New York, NY 10069 USA.
[Faraday, Nauder] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21205 USA.
[Ganesh, Santhi K.] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48108 USA.
[Ganesh, Santhi K.] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48108 USA.
[Auer, Paul L.] Univ Wisconsin, Zilber Sch Publ Hlth, Milwaukee, WI 53205 USA.
[Reiner, Alexander P.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA.
[Reiner, Alexander P.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA.
RP Lettre, G (reprint author), Univ Montreal, Dept Med, Montreal, PQ H3T 1J4, Canada.; Lettre, G (reprint author), Montreal Heart Inst, Montreal, PQ H1T 1CB, Canada.; Reiner, AP (reprint author), Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA.; Reiner, AP (reprint author), Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA.
EM apreiner@u.washington.edu; guillaume.lettre@umontreal.ca
RI Schurmann, Claudia/L-1204-2016; Floyd, James/G-7563-2015; Johnson,
Andrew/G-6520-2013; Kacprowski, Tim/K-8650-2013; Dube,
Marie-Pierre/B-9364-2008; Grarup, Niels/K-2807-2015;
OI Schurmann, Claudia/0000-0003-4158-9192; Evangelou,
Evangelos/0000-0002-5488-2999; Linneberg, Allan/0000-0002-0994-0184;
Kacprowski, Tim/0000-0002-5393-2413; Dube,
Marie-Pierre/0000-0001-8442-4393; Grarup, Niels/0000-0001-5526-1070;
Manichaikul, Ani/0000-0002-5998-795X; Tajuddin, Salman
M./0000-0002-7919-8528; Zonderman, Alan B/0000-0002-6523-4778
FU Fonds de Recherche du Queebec-Santee (FRQS); Canadian Institute of
Health Research (Banting-CIHR); Canadian Institute of Health Research
(operating grant MOP) [123382]; Canada Research Chair program; NHLBI
[R21 HL121422-02]; NIH [DK060022]; Yoshida Scholarship Foundation;
Research Scholar award from the Massachusetts General Hospital (MGH);
Howard Goodman Fellowship from MGH; Donovan Family Foundation
[R01HL107816]; Fondation Leducq
FX We thank all participants, staff, and study coordinating centers. We
also thank Raymond Doty and Jan Abkowitz for discussion of the ALAS2
finding. We would like to thank Liling Warren for contributions to the
genetic analysis of the SOLID-TIMI-52 and STABILITY datasets. Young
Finns Study (YFS) acknowledges the expert technical assistance in the
statistical analyses by Ville Aalto and Irina Lisinen. Estonian Genome
Center, University of Tartu (EGCUT) thanks co-workers at the Estonian
Biobank, especially Mr. V. Soo, Mr. S. Smith, and Dr. L. Milani. Airwave
thanks Louisa Cavaliero who assisted in data collection and management
as well as Peter McFarlane and the Glasgow CARE, Patricia Munroe at
Queen Mary University of London, and Joanna Sarnecka and Ania Zawodniak
at Northwick Park for their contributions to the study. This work was
supported by the Fonds de Recherche du Queebec-Santee (FRQS, scholarship
to N.C.), the Canadian Institute of Health Research (Banting-CIHR,
scholarship to S.L. and operating grant MOP#123382 to G.L.), and the
Canada Research Chair program (to G.L.). P.L.A. was supported by NHLBI
R21 HL121422-02. N.A.A. is funded by NIH DK060022. A.N. was supported by
the Yoshida Scholarship Foundation. S.K. was supported by a Research
Scholar award from the Massachusetts General Hospital (MGH), the Howard
Goodman Fellowship from MGH, the Donovan Family Foundation, R01HL107816,
and a grant from Fondation Leducq. Additional acknowledgments and
funding information is provided in the Supplemental Data.
NR 65
TC 3
Z9 3
U1 5
U2 6
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0002-9297
EI 1537-6605
J9 AM J HUM GENET
JI Am. J. Hum. Genet.
PD JUL 7
PY 2016
VL 99
IS 1
BP 8
EP 21
DI 10.1016/j.ajhg.2016.05.007
PG 14
WC Genetics & Heredity
SC Genetics & Heredity
GA DT6TI
UT WOS:000381616600002
PM 27346685
ER
PT J
AU Tajuddin, SM
Schick, UM
Eicher, JD
Chami, N
Giri, A
Brody, JA
Hill, WD
Kacprowski, T
Li, J
Lyytikainen, LP
Manichaikul, A
Mihailov, E
O'Donoghue, ML
Pankratz, N
Pazoki, R
Polfus, LM
Smith, AV
Schurmann, C
Vacchi-Suzzi, C
Waterworth, DM
Evangelou, E
Yanek, LR
Burt, A
Chen, MH
van Rooij, FJA
Floyd, JS
Greinacher, A
Harris, TB
Highland, HM
Lange, LA
Liu, Y
Magi, R
Nalls, MA
Mathias, RA
Nickerson, DA
Nikus, K
Starr, JM
Tardif, JC
Tzoulaki, I
Edwards, DRV
Wallentin, L
Bartz, TM
Becker, LC
Denny, JC
Raffield, LM
Rioux, JD
Friedrich, N
Fornage, M
Gao, H
Hirschhorn, JN
Liewald, DCM
Rich, SS
Uitterlinden, A
Bastarache, L
Becker, DM
Boerwinkle, E
de Denus, S
Bottinger, EP
Hayward, C
Hofman, A
Homuth, G
Lange, E
Launer, LJ
Lehtimaki, T
Lu, YC
Metspalu, A
O'Donnell, CJ
Quarells, RC
Richard, M
Torstenson, ES
Taylor, KD
Vergnaud, AC
Zonderman, AB
Crosslin, DR
Deary, IJ
Dorr, M
Elliott, P
Evans, MK
Gudnason, V
Kahonen, M
Psaty, BM
Rotter, JI
Slater, AJ
Dehghan, A
White, HD
Ganesh, SK
Loos, RJF
Esko, T
Faraday, N
Wilson, JG
Cushman, M
Johnson, AD
Edwards, TL
Zakai, NA
Lettre, G
Reiner, AP
Auer, PL
AF Tajuddin, Salman M.
Schick, Ursula M.
Eicher, John D.
Chami, Nathalie
Giri, Ayush
Brody, Jennifer A.
Hill, W. David
Kacprowski, Tim
Li, Jin
Lyytikainen, Leo-Pekka
Manichaikul, Ani
Mihailov, Evelin
O'Donoghue, Michelle L.
Pankratz, Nathan
Pazoki, Raha
Polfus, Linda M.
Smith, Albert Vernon
Schurmann, Claudia
Vacchi-Suzzi, Caterina
Waterworth, Dawn M.
Evangelou, Evangelos
Yanek, Lisa R.
Burt, Amber
Chen, Ming-Huei
van Rooij, Frank J. A.
Floyd, James S.
Greinacher, Andreas
Harris, Tamara B.
Highland, Heather M.
Lange, Leslie A.
Liu, Yongmei
Magi, Reedik
Nalls, Mike A.
Mathias, Rasika A.
Nickerson, Deborah A.
Nikus, Kjell
Starr, John M.
Tardif, Jean-Claude
Tzoulaki, Ioanna
Edwards, Digna R. Velez
Wallentin, Lars
Bartz, Traci M.
Becker, Lewis C.
Denny, Joshua C.
Raffield, Laura M.
Rioux, John D.
Friedrich, Nele
Fornage, Myriam
Gao, He
Hirschhorn, Joel N.
Liewald, David C. M.
Rich, Stephen S.
Uitterlinden, Andre
Bastarache, Lisa
Becker, Diane M.
Boerwinkle, Eric
de Denus, Simon
Bottinger, Erwin P.
Hayward, Caroline
Hofman, Albert
Homuth, Georg
Lange, Ethan
Launer, Lenore J.
Lehtimaki, Terho
Lu, Yingchang
Metspalu, Andres
O'Donnell, Chris J.
Quarells, Rakale C.
Richard, Melissa
Torstenson, Eric S.
Taylor, Kent D.
Vergnaud, Anne-Claire
Zonderman, Alan B.
Crosslin, David R.
Deary, Ian J.
Dorr, Marcus
Elliott, Paul
Evans, Michele K.
Gudnason, Vilmundur
Kahonen, Mika
Psaty, Bruce M.
Rotter, Jerome I.
Slater, Andrew J.
Dehghan, Abbas
White, Harvey D.
Ganesh, Santhi K.
Loos, Ruth J. F.
Esko, Tonu
Faraday, Nauder
Wilson, James G.
Cushman, Mary
Johnson, Andrew D.
Edwards, Todd L.
Zakai, Neil A.
Lettre, Guillaume
Reiner, Alex P.
Auer, Paul L.
TI Large-Scale Exome-wide Association Analysis Identifies Loci for White
Blood Cell Traits and Pleiotropy with Immune-Mediated Diseases
SO AMERICAN JOURNAL OF HUMAN GENETICS
LA English
DT Article
ID ACUTE MYELOID-LEUKEMIA; UROKINASE RECEPTOR; GENETIC-VARIANTS;
LYMPHOBLASTIC-LEUKEMIA; TRANSCRIPTION FACTORS; SUSCEPTIBILITY LOCI;
LYMPHOID ORGANS; EMERGE NETWORK; RISK LOCI; EXPRESSION
AB White blood cells play diverse roles in innate and adaptive immunity. Genetic association analyses of phenotypic variation in circulating white blood cell (WBC) counts from large samples of otherwise healthy individuals can provide insights into genes and biologic pathways involved in production, differentiation, or clearance of particular WBC lineages (myeloid, lymphoid) and also potentially inform the genetic basis of autoimmune, allergic, and blood diseases. We performed an exome array-based meta-analysis of total WBC and subtype counts (neutrophils, monocytes, lymphocytes, basophils, and eosinophils) in a multi-ancestry discovery and replication sample of similar to 157,622 individuals from 25 studies. We identified 16 common variants (8 of which were coding variants) associated with one or more WBC traits, the majority of which are pleiotropically associated with autoimmune diseases. Based on functional annotation, these loci included genes encoding surface markers of myeloid, lymphoid, or hematopoietic stem cell differentiation (CD69, CD33, CD87), transcription factors regulating lineage specification during hematopoiesis (ASXL1, IRF8, IKZF1, JMJD1C, ETS2-PSMG1), and molecules involved in neutrophil clearance/apoptosis (C10orf54, LTA), adhesion (TNXB), or centrosome and microtubule structure/function (KIF9, TUBD1). Together with recent reports of somatic ASXL1 mutations among individuals with idiopathic cytopenias or clonal hematopoiesis of undetermined significance, the identification of a common regulatory 3 ' UTR variant of ASXL1 suggests that both germline and somatic ASXL1 mutations contribute to lower blood counts in otherwise asymptomatic individuals. These association results shed light on genetic mechanisms that regulate circulating WBC counts and suggest a prominent shared genetic architecture with inflammatory and autoimmune diseases.
C1 [Tajuddin, Salman M.; Zonderman, Alan B.; Evans, Michele K.] NIA, Lab Epidemiol & Populat Sci, NIH, Baltimore, MD 21224 USA.
[Schick, Ursula M.; Schurmann, Claudia; Bottinger, Erwin P.; Lu, Yingchang; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, New York, NY 10029 USA.
[Schick, Ursula M.; Schurmann, Claudia; Lu, Yingchang; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Genet Obes & Related Metab Traits Program, New York, NY 10029 USA.
[Eicher, John D.; Chen, Ming-Huei; Johnson, Andrew D.] NHLBI, Populat Sci Branch, Framingham Heart Study, Framingham, MA 01702 USA.
[Chami, Nathalie; Tardif, Jean-Claude; Rioux, John D.; Lettre, Guillaume] Univ Montreal, Dept Med, Montreal, PQ H3T 1J4, Canada.
[Chami, Nathalie; Tardif, Jean-Claude; Rioux, John D.; de Denus, Simon; Lettre, Guillaume] Montreal Heart Inst, Montreal, PQ H1T 1C8, Canada.
[Giri, Ayush; Torstenson, Eric S.] Vanderbilt Univ, Div Epidemiol, Inst Med & Publ Hlth, Nashville, TN 37235 USA.
[Brody, Jennifer A.; Floyd, James S.] Univ Washington, Dept Med, Seattle, WA 98101 USA.
[Hill, W. David; Starr, John M.; Liewald, David C. M.; Deary, Ian J.] Univ Edinburgh, Ctr Cognit Ageing & Cognit Epidemiol, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Hill, W. David; Liewald, David C. M.; Deary, Ian J.] Univ Edinburgh, Dept Psychol, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Kacprowski, Tim; Homuth, Georg] Univ Med Greifswald, Interfac Inst Genet & Funct Genom, Dept Funct Genom, D-17475 Greifswald, Germany.
[Kacprowski, Tim; Homuth, Georg] Ernst Moritz Arndt Univ Greifswald, D-17475 Greifswald, Germany.
[Kacprowski, Tim; Friedrich, Nele; Dorr, Marcus] DZHK German Ctr Cardiovasc Res, Greifswald, Germany.
[Li, Jin] Stanford Univ, Sch Med, Dept Med, Div Cardiovasc Med, Palo Alto, CA 94305 USA.
[Lyytikainen, Leo-Pekka; Lehtimaki, Terho] Fimlab Labs, Dept Clin Chem, Tampere 33520, Finland.
[Lyytikainen, Leo-Pekka; Lehtimaki, Terho] Univ Tampere, Sch Med, Dept Clin Chem, Tampere 33014, Finland.
[Manichaikul, Ani; Rich, Stephen S.] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA 22908 USA.
[Mihailov, Evelin; Magi, Reedik; Metspalu, Andres; Esko, Tonu] Univ Tartu, Estonian Genome Ctr, EE-51010 Tartu, Estonia.
[O'Donoghue, Michelle L.] Brigham & Womens Hosp, Div Cardiovasc, TIMI Study Grp, Boston, MA 02115 USA.
[Pankratz, Nathan] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55454 USA.
[Pazoki, Raha; van Rooij, Frank J. A.; Uitterlinden, Andre; Hofman, Albert; Dehghan, Abbas] Erasmus Univ, Med Ctr, Dept Epidemiol, NL-3000 DR Rotterdam, Netherlands.
[Polfus, Linda M.; Highland, Heather M.; Boerwinkle, Eric] Univ Texas Hlth Sci Ctr Houston, Sch Publ Hlth, Ctr Human Genet, Houston, TX 77030 USA.
[Smith, Albert Vernon; Gudnason, Vilmundur] Iceland Heart Assoc, IS-201 Kopavogur, Iceland.
[Smith, Albert Vernon; Gudnason, Vilmundur] Univ Iceland, Fac Med, IS-101 Reykjavik, Iceland.
[Vacchi-Suzzi, Caterina] SUNY Stony Brook, Dept Family Populat & Prevent Med, Stony Brook, NY 11794 USA.
[Waterworth, Dawn M.] GlaxoSmithKline, Target Sci, Genet, King Of Prussia, PA 19406 USA.
[Evangelou, Evangelos; Tzoulaki, Ioanna; Gao, He; Vergnaud, Anne-Claire; Elliott, Paul] Imperial Coll London, Sch Publ Hlth, MRC PHE Ctr Environm & Hlth, Dept Epidemiol & Biostat, London W2 1PG, England.
[Evangelou, Evangelos; Tzoulaki, Ioanna] Univ Ioannina, Sch Med, Dept Hyg & Epidemiol, GR-45110 Ioannina, Greece.
[Yanek, Lisa R.; Becker, Diane M.] Johns Hopkins Univ, Sch Med, Dept Med, Div Gen Internal Med, Baltimore, MD 21205 USA.
[Burt, Amber] Univ Washington, Dept Med, Div Med Genet, Seattle, WA 98195 USA.
[Greinacher, Andreas] Univ Med Greifswald, Inst Immunol & Transfus Med, D-17475 Greifswald, Germany.
[Harris, Tamara B.; Launer, Lenore J.] NIA, Lab Epidemiol Demog & Biometry, Intramural Res Program, NIH, Bethesda, MD 20892 USA.
[Highland, Heather M.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27514 USA.
[Lange, Leslie A.; Raffield, Laura M.] Univ N Carolina, Dept Genet, Chapel Hill, NC 27514 USA.
[Liu, Yongmei] Wake Forest Sch Med, Div Publ Hlth Sci, Ctr Human Genet, Winston Salem, NC 27157 USA.
[Nalls, Mike A.] NIA, Neurogenet Lab, NIH, Bethesda, MD 20892 USA.
[Mathias, Rasika A.] Johns Hopkins Univ, Sch Med, Dept Med, Div Allergy & Clin Immunol, Baltimore, MD 21205 USA.
[Mathias, Rasika A.; Becker, Lewis C.] Johns Hopkins Univ, Sch Med, Dept Med, Div Gen Internal Med, Baltimore, MD 21205 USA.
[Nickerson, Deborah A.] Univ Washington, Sch Med, Dept Genome Sci, Seattle, WA 98105 USA.
[Nikus, Kjell] Tampere Univ Hosp, Dept Cardiol, Ctr Heart, Tampere 33521, Finland.
[Nikus, Kjell] Univ Tampere, Sch Med, Tampere 33014, Finland.
[Starr, John M.] Alzheimer Scotland Dementia Res Ctr, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Edwards, Digna R. Velez] Vanderbilt Univ, Vanderbilt Genet Inst, Inst Med & Publ Hlth, Vanderbilt Epidemiol Ctr,Dept Obstet & Gynecol, Nashville, TN 37203 USA.
[Wallentin, Lars] Uppsala Univ, Cardiol, Dept Med Sci, S-75185 Uppsala, Sweden.
[Wallentin, Lars] Uppsala Univ, Uppsala Clin Res Ctr, S-75185 Uppsala, Sweden.
[Bartz, Traci M.] Univ Washington, Dept Biostat, Seattle, WA 98195 USA.
[Becker, Lewis C.] Johns Hopkins Univ, Sch Med, Dept Med, Div Cardiol, Baltimore, MD 21205 USA.
[Denny, Joshua C.; Bastarache, Lisa] Vanderbilt Univ, Sch Med, Dept Biomed Informat, Nashville, TN 37203 USA.
[Friedrich, Nele] Univ Med Greifswald, Inst Clin Chem & Lab Med, D-13347 Greifswald, Germany.
[Fornage, Myriam; Richard, Melissa] Univ Texas Hlth Sci Ctr Houston, Inst Mol Med, Houston, TX 77030 USA.
[Hirschhorn, Joel N.; Esko, Tonu] Broad Inst, Program Med & Populat Genet, Cambridge, MA 02142 USA.
[Hirschhorn, Joel N.] Boston Childrens Hosp, Dept Endocrinol, Boston, MA 02115 USA.
[Uitterlinden, Andre] Erasmus Univ, Med Ctr, Dept Internal Med, NL-3000 DR Rotterdam, Netherlands.
[Uitterlinden, Andre] NCHA, NL-3015 GD Rotterdam, Netherlands.
[Boerwinkle, Eric] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA.
[de Denus, Simon] Univ Montreal, Fac Pharm, Montreal, PQ H3T 1J4, Canada.
[Hayward, Caroline] Univ Edinburgh, Inst Genet & Mol Med, MRC Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland.
[Hofman, Albert] Harvard TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA.
[Lange, Ethan] Univ N Carolina, Dept Genet & Biostat, Chapel Hill, NC 27599 USA.
[O'Donnell, Chris J.] NHLBI, Framingham Heart Study, Framingham, MA 01702 USA.
[O'Donnell, Chris J.] Boston Vet Adm Healthcare, Cardiol Sect, Boston, MA 02118 USA.
[O'Donnell, Chris J.] Boston Vet Adm Healthcare, Ctr Populat Genom, Boston, MA 02118 USA.
[Quarells, Rakale C.] Morehouse Sch Med, Social Epidemiol Res Ctr, Cardiovasc Res Inst, Atlanta, GA 30310 USA.
[Taylor, Kent D.; Rotter, Jerome I.] Los Angeles Biomed Res Inst, Inst Translat Genom & Populat Sci, Torrance, CA 90502 USA.
[Taylor, Kent D.; Rotter, Jerome I.] Harbor UCLA Med Ctr, Dept Pediat, Torrance, CA 90502 USA.
[Crosslin, David R.] Univ Washington, Dept Biomed Informat & Med Educ, Seattle, WA 98195 USA.
[Dorr, Marcus] Univ Med Greifswald, Dept Cardiol, D-17475 Greifswald, Germany.
[Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere 33521, Finland.
[Kahonen, Mika] Univ Tampere, Sch Med, Dept Clin Physiol, Tampere 33014, Finland.
[Psaty, Bruce M.] Univ Washington, Cardiovasc Hlth Res Unit, Dept Epidemiol, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Univ Washington, Cardiovasc Hlth Res Unit, Dept Hlth Serv, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Univ Washington, Cardiovasc Hlth Res Unit, Dept Med, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Grp Hlth Cooperat Puget Sound, Grp Hlth Res Inst, Seattle, WA 98101 USA.
[Slater, Andrew J.] OmicSoft Corp, Cary, NC 27513 USA.
[Slater, Andrew J.] GlaxoSmithKline, Genet Target Sci, Res Triangle Pk, NC 27709 USA.
[White, Harvey D.] Auckland City Hosp, Green Lane Cardiovasc Serv, Auckland 1142, New Zealand.
[White, Harvey D.] Univ Auckland, Auckland 1142, New Zealand.
[Ganesh, Santhi K.] Univ Michigan, Dept Internal Med, Ann Arbor, MI 48108 USA.
[Ganesh, Santhi K.] Univ Michigan, Dept Human Genet, Ann Arbor, MI 48108 USA.
[Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Mindich Child Hlth & Dev Inst, New York, NY 10029 USA.
[Faraday, Nauder] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21205 USA.
[Wilson, James G.] Univ Mississippi, Med Ctr, Dept Physiol & Biophys, Jackson, MS 39216 USA.
[Cushman, Mary; Zakai, Neil A.] Univ Vermont, Dept Med, Div Hematol Oncol, Colchester, VT 05446 USA.
[Edwards, Todd L.] Vanderbilt Univ, Vanderbilt Genet Inst, Inst Med & Publ Hlth, Div Epidemiol,Dept Med, Nashville, TN 37203 USA.
[Reiner, Alex P.] Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA.
[Reiner, Alex P.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA.
[Auer, Paul L.] Univ Wisconsin Milwaukee, Zilber Sch Publ Hlth, Milwaukee, WI 53205 USA.
RP Reiner, AP (reprint author), Univ Washington, Dept Epidemiol, Seattle, WA 98195 USA.; Reiner, AP (reprint author), Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA.; Auer, PL (reprint author), Univ Wisconsin Milwaukee, Zilber Sch Publ Hlth, Milwaukee, WI 53205 USA.
EM apreiner@u.washington.edu; pauer@uwm.edu
RI Schurmann, Claudia/L-1204-2016; Floyd, James/G-7563-2015; Rioux,
John/A-9599-2015; Johnson, Andrew/G-6520-2013; Kacprowski,
Tim/K-8650-2013;
OI Schurmann, Claudia/0000-0003-4158-9192; Rioux, John/0000-0001-7560-8326;
Kacprowski, Tim/0000-0002-5393-2413; Manichaikul,
Ani/0000-0002-5998-795X; Tajuddin, Salman M./0000-0002-7919-8528;
Zonderman, Alan B/0000-0002-6523-4778; Evangelou,
Evangelos/0000-0002-5488-2999; Dehghan, Abbas/0000-0001-6403-016X
FU NHLBI [R21 HL121422-02, R01 HL086694]; Canada Research Chair program;
Canadian Institute of Health Research MOP [123382]; National Institute
on Aging, NIH Intramural Research Program; a component of the NIH
Roadmap for Medical Research [UL1RR025005]
FX We thank all participants and study coordinating centers of the
participating studies and cohorts. P.L.A. was supported by NHLBI R21
HL121422-02. G.L. was supported by the Canada Research Chair program and
the Canadian Institute of Health Research MOP#123382. This work was
supported in part by the National Institute on Aging, NIH Intramural
Research Program. Data analyses utilized the computational resources of
the NIH HPC Biowulf cluster at the NIH. The Framingham Heart Study (FHS)
acknowledges the Shared Computing Cluster at Boston University.
Infrastructure and data analysis for the ARIC study was partly supported
by Grant Number UL1RR025005, a component of the NIH Roadmap for Medical
Research, and grant R01 HL086694 from the NHLBI. Airwave study thanks
Louisa Cavaliero who assisted in data collection and management, Peter
McFarlane and the Glasgow CARE, Patricia Munroe at Queen Mary University
of London, and Joanna Sarnecka and Ania Zawodniak at Northwick Park for
their contributions to the study. SOLID-TIMI-52 and STABILITY thank
Liling Warren for contributions to the genetic analysis of the study
datasets. Estonian Genome Center, University of Tartu (EGCUT) thanks Mr.
V. Soo, Mr. S. Smith, and Dr. L. Milani for their contribution. The
Rotterdam Study (RS) thanks Ms. Mila Jhamai, Ms. Sarah Higgins, and Mr.
Marijn Verkerk for their help in creating the Exomechip database, and
Ms. Carolina MedinaGomez, Mr. Lennard Karsten, and Dr. Linda Broer.
Additional acknowledgments and funding information are provided in the
Supplemental Data.
NR 96
TC 3
Z9 3
U1 7
U2 8
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0002-9297
EI 1537-6605
J9 AM J HUM GENET
JI Am. J. Hum. Genet.
PD JUL 7
PY 2016
VL 99
IS 1
BP 22
EP 39
DI 10.1016/j.ajhg.2016.05.003
PG 18
WC Genetics & Heredity
SC Genetics & Heredity
GA DT6TI
UT WOS:000381616600003
PM 27346689
ER
PT J
AU Eicher, JD
Chami, N
Kacprowski, T
Nomura, A
Chen, MH
Yanek, LR
Tajuddin, SM
Schick, UM
Slater, AJ
Pankratz, N
Polfus, L
Schurmann, C
Giri, A
Brody, JA
Lange, LA
Manichaikul, A
Hill, WD
Pazoki, R
Elliot, P
Evangelou, E
Tzoulaki, I
Gao, H
Vergnaud, AC
Mathias, RA
Becker, DM
Becker, LC
Burt, A
Crosslin, DR
Lyytikainen, LP
Nikus, K
Hernesniemi, J
Kahonen, M
Raitoharju, E
Mononen, N
Raitakari, OT
Lehtimaki, T
Cushman, M
Zakai, NA
Nickerson, DA
Raffield, LM
Quarells, R
Willer, CJ
Peloso, GM
Abecasis, GR
Liu, DJJ
Deloukas, P
Samani, NJ
Schunkert, H
Erdmann, J
Fornage, M
Richard, M
Tardif, JC
Rioux, JD
Dube, MP
de Denus, S
Lu, YC
Bottinger, EP
Loos, RJF
Smith, AV
Harris, TB
Launer, LJ
Gudnason, V
Edwards, DRV
Torstenson, ES
Liu, YM
Tracy, RP
Rotter, JI
Rich, SS
Highland, HM
Boerwinkle, E
Li, J
Lange, E
Wilson, JG
Mihailov, E
Magi, R
Hirschhorn, J
Metspalu, A
Esko, T
Vacchi-Suzzi, C
Nalls, MA
Zonderman, AB
Evans, MK
Engstrom, G
Orho-Melander, M
Melander, O
O'Donoghue, ML
Waterworth, DM
Wallentin, L
White, HD
Floyd, JS
Bartz, TM
Rice, KM
Psaty, BM
Starr, JM
Liewald, DCM
Hayward, C
Deary, IJ
Greinacher, A
Volker, U
Thiele, T
Volzke, H
van Rooij, FJA
Uitterlinden, AG
Franco, OH
Dehghan, A
Edwards, TL
Ganesh, SK
Kathiresan, S
Faraday, N
Auer, PL
Reiner, AP
Lettre, G
Johnson, AD
AF Eicher, John D.
Chami, Nathalie
Kacprowski, Tim
Nomura, Akihiro
Chen, Ming-Huei
Yanek, Lisa R.
Tajuddin, Salman M.
Schick, Ursula M.
Slater, Andrew J.
Pankratz, Nathan
Polfus, Linda
Schurmann, Claudia
Giri, Ayush
Brody, Jennifer A.
Lange, Leslie A.
Manichaikul, Ani
Hill, W. David
Pazoki, Raha
Elliot, Paul
Evangelou, Evangelos
Tzoulaki, Ioanna
Gao, He
Vergnaud, Anne-Claire
Mathias, Rasika A.
Becker, Diane M.
Becker, Lewis C.
Burt, Amber
Crosslin, David R.
Lyytikainen, Leo-Pekka
Nikus, Kjell
Hernesniemi, Jussi
Kahonen, Mika
Raitoharju, Emma
Mononen, Nina
Raitakari, Olli T.
Lehtimaki, Terho
Cushman, Mary
Zakai, Neil A.
Nickerson, Deborah A.
Raffield, Laura M.
Quarells, Rakale
Willer, Cristen J.
Peloso, Gina M.
Abecasis, Goncalo R.
Liu, Dajiang J.
Deloukas, Panos
Samani, Nilesh J.
Schunkert, Heribert
Erdmann, Jeanette
Fornage, Myriam
Richard, Melissa
Tardif, Jean-Claude
Rioux, John D.
Dube, Marie-Pierre
de Denus, Simon
Lu, Yingchang
Bottinger, Erwin P.
Loos, Ruth J. F.
Smith, Albert Vernon
Harris, Tamara B.
Launer, Lenore J.
Gudnason, Vilmundur
Edwards, Digna R. Velez
Torstenson, Eric S.
Liu, Yongmei
Tracy, Russell P.
Rotter, Jerome I.
Rich, Stephen S.
Highland, Heather M.
Boerwinkle, Eric
Li, Jin
Lange, Ethan
Wilson, James G.
Mihailov, Evelin
Magi, Reedik
Hirschhorn, Joel
Metspalu, Andres
Esko, Tonu
Vacchi-Suzzi, Caterina
Nalls, Mike A.
Zonderman, Alan B.
Evans, Michele K.
Engstrom, Gunnar
Orho-Melander, Marju
Melander, Olle
O'Donoghue, Michelle L.
Waterworth, Dawn M.
Wallentin, Lars
White, Harvey D.
Floyd, James S.
Bartz, Traci M.
Rice, Kenneth M.
Psaty, Bruce M.
Starr, J. M.
Liewald, David C. M.
Hayward, Caroline
Deary, Ian J.
Greinacher, Andreas
Volker, Uwe
Thiele, Thomas
Volzke, Henry
van Rooij, Frank J. A.
Uitterlinden, Andre G.
Franco, Oscar H.
Dehghan, Abbas
Edwards, Todd L.
Ganesh, Santhi K.
Kathiresan, Sekar
Faraday, Nauder
Auer, Paul L.
Reiner, Alex P.
Lettre, Guillaume
Johnson, Andrew D.
CA Global Lipids Genetics Consortium
CARDIoGRAM Exome Consortium
Myocardial Infarction Genetics
TI Platelet-Related Variants Identified by Exomechip Meta-analysis in
157,293 Individuals
SO AMERICAN JOURNAL OF HUMAN GENETICS
LA English
DT Article
ID GENOME-WIDE ASSOCIATION; EPSILON-RI-ALPHA; PIAS-LIKE PROTEIN; IMMUNE
THROMBOCYTOPENIA; MYOCARDIAL-INFARCTION; AFRICAN-AMERICANS; SEQUENCE
VARIANTS; BLOOD-PRESSURE; BINDING-SITE; IGE BINDING
AB Platelet production, maintenance, and clearance are tightly controlled processes indicative of platelets' important roles in hemostasis and thrombosis. Platelets are common targets for primary and secondary prevention of several conditions. They are monitored clinically by complete blood counts, specifically with measurements of platelet count (PLT) and mean platelet volume (MPV). Identifying genetic effects on PLT and MPV can provide mechanistic insights into platelet biology and their role in disease. Therefore, we formed the Blood Cell Consortium (BCX) to perform a large-scale meta-analysis of Exomechip association results for PLT and MPV in 157,293 and 57,617 individuals, respectively. Using the low-frequency/rare coding variant-enriched Exomechip genotyping array, we sought to identify genetic variants associated with PLT and MPV. In addition to confirming 47 known PLT and 20 known MPV associations, we identified 32 PLT and 18 MPV associations not previously observed in the literature across the allele frequency spectrum, including rare large effect (FCER1A), low-frequency (IQGAP2, MAP1A, LY75), and common(ZMIZ2, SMG6, PEAR1, ARFGAP3/PACSIN2) variants. Several variants associated with PLT/MPV(PEAR1, MRVI1, PTGES3) were also associated with platelet reactivity. In concurrent BCX analyses, there was overlap of platelet-associated variants with red (MAP1A, TMPRSS6, ZMIZ2) and white (PEAR1, ZMIZ2, LY75) blood cell traits, suggesting common regulatory pathways with shared genetic architecture among these hematopoietic lineages. Our large-scale Exomechip analyses identified previously undocumented associations with platelet traits and further indicate that several complex quantitative hematological, lipid, and cardiovascular traits share genetic factors.
C1 [Eicher, John D.; Chen, Ming-Huei; Johnson, Andrew D.] NHLBI, Populat Sci Branch, Framingham Heart Study, Framingham, MA 01702 USA.
[Chami, Nathalie; Tardif, Jean-Claude; Rioux, John D.; Dube, Marie-Pierre; Lettre, Guillaume] Univ Montreal, Dept Med, Montreal, PQ H3T 1J4, Canada.
[Chami, Nathalie; Tardif, Jean-Claude; Rioux, John D.; Dube, Marie-Pierre; de Denus, Simon; Lettre, Guillaume] Montreal Heart Inst, Montreal, PQ H1T 1C8, Canada.
[Kacprowski, Tim; Volker, Uwe] Univ Med Greifswald, Interfac Inst Genet & Funct Genom, Dept Funct Genom, D-17475 Greifswald, Germany.
[Kacprowski, Tim; Volker, Uwe] Ernst Mortiz Arndt Univ Greifswald, D-17475 Greifswald, Germany.
[Kacprowski, Tim; Volker, Uwe; Volzke, Henry] DZHK German Ctr Cardiovasc Res, Partner Site Greifswald, Greifswald, Germany.
[Nomura, Akihiro; Peloso, Gina M.; Kathiresan, Sekar] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA.
[Nomura, Akihiro; Peloso, Gina M.; Hirschhorn, Joel; Esko, Tonu; Kathiresan, Sekar] Broad Inst, Program Med & Populat Genet, Cambridge, MA 02142 USA.
[Nomura, Akihiro; Kathiresan, Sekar] Massachusetts Gen Hosp, Cardiovasc Res Ctr, Boston, MA 02114 USA.
[Nomura, Akihiro; Kathiresan, Sekar] Harvard Med Sch, Dept Med, Boston, MA 02115 USA.
[Nomura, Akihiro] Kanazawa Univ, Div Cardiovasc Med, Grad Sch Med Sci, Kanazawa, Ishikawa 9200942, Japan.
[Yanek, Lisa R.; Mathias, Rasika A.; Becker, Diane M.; Becker, Lewis C.] Johns Hopkins Univ, Div Gen Internal Med, Dept Med, Sch Med, Baltimore, MD 21205 USA.
[Tajuddin, Salman M.; Zonderman, Alan B.; Evans, Michele K.] NIA, Lab Epidemiol & Populat Sci, NIH, Baltimore, MD 21224 USA.
[Schick, Ursula M.; Schurmann, Claudia; Lu, Yingchang; Bottinger, Erwin P.; Loos, Ruth J. F.] Icahn Sch Med Mt Sinai, Charles Bronfman Inst Personalized Med, New York, NY 10029 USA.
[Schick, Ursula M.; Schurmann, Claudia] Icahn Sch Med Mt Sinai, Genet Obes & Related Metab Traits Program, New York, NY 10029 USA.
[Slater, Andrew J.] GlaxoSmithKline, Genet, Target Sci, Res Triangle Pk, NC 27709 USA.
[Slater, Andrew J.] OmicSoft Corp, Cary, NC 27513 USA.
[Pankratz, Nathan] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55454 USA.
[Polfus, Linda; Boerwinkle, Eric] Univ Texas Hlth Sci Ctr Houston, Sch Publ Hlth, Ctr Human Genet, Houston, TX 77030 USA.
[Giri, Ayush; Torstenson, Eric S.; Edwards, Todd L.] Vanderbilt Univ, Inst Med & Publ Hlth, Div Epidemiol, Nashville, TN 37235 USA.
[Brody, Jennifer A.; Floyd, James S.] Univ Washington, Dept Med, Seattle, WA 98101 USA.
[Lange, Leslie A.; Raffield, Laura M.; Lange, Ethan] Univ N Carolina, Dept Genet, Chapel Hill, NC 27514 USA.
[Manichaikul, Ani; Rich, Stephen S.] Univ Virginia, Ctr Publ Hlth Gen, Charlottesville, VA 22908 USA.
[Hill, W. David; Starr, J. M.; Liewald, David C. M.; Deary, Ian J.] Univ Edinburgh, Ctr Cognit Ageing & Cognit Epidemiol, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Hill, W. David; Liewald, David C. M.; Deary, Ian J.] Univ Edinburgh, Dept Psychol, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Pazoki, Raha; van Rooij, Frank J. A.; Uitterlinden, Andre G.; Franco, Oscar H.; Dehghan, Abbas] Erasmus MC, Dept Epidemiol, NL-3000 Rotterdam, Netherlands.
[Elliot, Paul; Evangelou, Evangelos; Tzoulaki, Ioanna; Gao, He; Vergnaud, Anne-Claire] Univ London Imperial Coll Sci Technol & Med, MRC PHE Ctr Environm & Hlth, Dept Epidemiol & Biostat, Sch Publ Hlth, London W2 1PG, England.
[Evangelou, Evangelos; Tzoulaki, Ioanna] Univ Ioannina, Dept Hyg & Epidemiol, Sch Med, Ioannina 45110, Greece.
[Mathias, Rasika A.] Johns Hopkins Univ, Sch Med, Dept Med, Div Allergy, Baltimore, MD 21205 USA.
[Mathias, Rasika A.] Johns Hopkins Univ, Sch Med, Dept Med, Div Immunol, Baltimore, MD 21205 USA.
[Becker, Lewis C.] Johns Hopkins Univ, Div Cardiol, Dept Med, Sch Med, Baltimore, MD 21205 USA.
[Burt, Amber] Univ Washington, Div Med Genet, Dept Med, Seattle, WA 98195 USA.
[Crosslin, David R.] Univ Washington, Dept Biomed Informat & Med Educ, Seattle, WA 98105 USA.
[Lyytikainen, Leo-Pekka; Hernesniemi, Jussi; Raitoharju, Emma; Mononen, Nina; Lehtimaki, Terho] Fimlab Labs, Dept Clin Chem, Tampere 33520, Finland.
[Lyytikainen, Leo-Pekka; Hernesniemi, Jussi; Raitoharju, Emma; Mononen, Nina; Lehtimaki, Terho] Univ Tampere, Dept Clin Chem, Sch Med, Tampere 33514, Finland.
[Nikus, Kjell; Hernesniemi, Jussi] Tampere Univ Hosp, Dept Cardiol, Ctr Heart, Tampere 33521, Finland.
[Nikus, Kjell] Univ Tampere, Sch Med, Tampere 33514, Finland.
[Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere 33521, Finland.
[Kahonen, Mika] Univ Tampere, Dept Clin Physiol, Tampere 33514, Finland.
[Raitakari, Olli T.] Turku Univ Hosp, Dept Clin Physiol & Nucl Med, Turku 20521, Finland.
[Raitakari, Olli T.] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, FIN-20520 Turku, Finland.
[Cushman, Mary; Zakai, Neil A.] Univ Vermont, Coll Med, Dept Med, Burlington, VT 05405 USA.
[Cushman, Mary; Zakai, Neil A.] Univ Vermont, Coll Med, Dept Pathol, Burlington, VT 05405 USA.
[Nickerson, Deborah A.] Univ Washington, Dept Genome Sci, Seattle, WA 98105 USA.
[Quarells, Rakale] Morehouse Sch Med, Social Epidemiol Res Ctr, Cardiovasc Res Inst, Atlanta, GA 30310 USA.
[Willer, Cristen J.] Univ Michigan, Dept Internal Med, Div Cardiovasc Med, Ann Arbor, MI 48108 USA.
[Willer, Cristen J.] Univ Michigan, Dept Computat Med & Bioinformat, Dept Human Genet, Ann Arbor, MI 48108 USA.
[Willer, Cristen J.] Univ Michigan, Dept Biostat, Ann Arbor, MI 48108 USA.
[Peloso, Gina M.] Boston Univ, Dept Biostat, Sch Publ Hlth, Boston, MA 02118 USA.
[Abecasis, Goncalo R.] Univ Michigan, Dept Biostat, Ctr Stat Genet, Ann Arbor, MI 48108 USA.
[Liu, Dajiang J.] Penn State Univ Hosp, Dept Publ Hlth Sci, Coll Med, Hershey, PA 17033 USA.
[Deloukas, Panos] Queen Mary Univ London, William Harvey Res Inst, London E1 4NS, England.
[Deloukas, Panos] King Abdulaziz Univ, Princess Al Jawhara Al Brahim Ctr Excellence Res, Jeddah 21589, Saudi Arabia.
[Samani, Nilesh J.] Univ Leicester, Dept Cardiovasc Sci, Leicester LE1 7RH, Leics, England.
[Samani, Nilesh J.] Glenfield Hosp, NIHR Leicester Cardiovasc Biomed Res Unit, Leicester LE3 9QP, Leics, England.
[Schunkert, Heribert] DZHK German Ctr Cardiovasc Res, Partner Site Munich Heart Alliance, D-80333 Munich, Germany.
[Schunkert, Heribert] Tech Univ Munich, Deutsch Herzzentrum Munchen, D-80333 Munich, Germany.
[Erdmann, Jeanette] Univ Lubeck, Inst Integrat & Expt Genom, D-23562 Lubeck, Germany.
[Erdmann, Jeanette] DZHK German Res Ctr Cardiovasc Res, Partner Site Hamburg Lubeck Kiel, D-23562 Lubeck, Germany.
[Fornage, Myriam; Richard, Melissa] Univ Texas Hlth Sci Ctr Houston, Inst Mol Med, Houston, TX 77030 USA.
[de Denus, Simon] Univ Montreal, Fac Pharm, Montreal H3T 1J4, PQ, Canada.
[Smith, Albert Vernon; Gudnason, Vilmundur] Icelandic Heart Assoc, IS-201 Kopavogur, Iceland.
[Smith, Albert Vernon; Gudnason, Vilmundur] Univ Iceland, Fac Med, IS-101 Reykjavik, Iceland.
[Harris, Tamara B.; Launer, Lenore J.] NIA, Lab Epidemiol Demog & Biometry, Intramural Res Program, NIH, Baltimore, MD 21224 USA.
[Edwards, Digna R. Velez] Vanderbilt Univ, Vanderbilt Epidemiol Ctr, Dept Obstet & Gynecol, Inst Med & Publ Hlth,Vanderbilt Genet Inst, Nashville, TN 37203 USA.
[Liu, Yongmei] Wake Forest Sch Med, Div Publ Hlth Sci, Ctr Human Genet, Winston Salem, NC 27157 USA.
[Tracy, Russell P.] Univ Vermont, Coll Med, Dept Pathol & Lab Med, Colchester, VT 05446 USA.
[Tracy, Russell P.] Univ Vermont, Coll Med, Dept Biochem, Colchester, VT 05446 USA.
[Rotter, Jerome I.] Los Angeles Biomed Res Inst, Inst Translat Genom & Populat Sci, Torrance, CA 90502 USA.
[Rotter, Jerome I.] Harbor UCLA Med Ctr, Dept Pediat, Torrance, CA 90502 USA.
[Highland, Heather M.] Univ Texas Hlth Sci Ctr Houston, Univ Texas Grad Sch Biomed Sci Houston, Univ Texas Sch Publ Hlth, Houston, TX 77030 USA.
[Highland, Heather M.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27514 USA.
[Boerwinkle, Eric] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA.
[Li, Jin] Stanford Univ, Sch Med, Dept Med, Div Cardiovasc Med, Palo Alto, CA 94305 USA.
[Lange, Ethan] Univ N Carolina, Dept Biostat, Chapel Hill, NC 27514 USA.
[Wilson, James G.] Univ Mississippi, Dept Physiol & Biophys, Med Ctr, Jackson, MS 39216 USA.
[Mihailov, Evelin; Magi, Reedik; Metspalu, Andres; Esko, Tonu] Univ Tartu, Estonian Genome Ctr, EE-51010 Tartu, Estonia.
[Hirschhorn, Joel] Boston Childrens Hosp, Dept Endocrinol, Boston, MA 02115 USA.
[Vacchi-Suzzi, Caterina] SUNY Stony Brook, Dept Family Populat & Prevent Med, Stony Brook, NY 11794 USA.
[Nalls, Mike A.] NIA, Neurogenet Lab, NIH, Bethesda, MD 21224 USA.
[Engstrom, Gunnar; Orho-Melander, Marju; Melander, Olle] Lund Univ, Dept Clin Sci Malmo, S-22100 Malmo, Sweden.
[Engstrom, Gunnar; Orho-Melander, Marju; Melander, Olle] Skane Univ Hosp, S-22241 Malmo, Sweden.
[O'Donoghue, Michelle L.] Brigham & Womens Hosp, TIMI Study Grp, Div Cardiovasc, Boston, MA 02115 USA.
[Waterworth, Dawn M.] GlaxoSmithKline, Target Sci, Genet, King Of Prussia, PA 19406 USA.
[Wallentin, Lars] Uppsala Univ, Dept Med Sci, Cardiol, S-75185 Uppsala, Sweden.
[Wallentin, Lars] Uppsala Univ, Dept Med Sci, Uppsala Clin Res Ctr, S-75185 Uppsala, Sweden.
[White, Harvey D.] Auckland City Hosp, Green Lane Cardiovasc Serv, Auckland 1142, New Zealand.
[White, Harvey D.] Univ Auckland, Auckland 1142, New Zealand.
[Bartz, Traci M.; Rice, Kenneth M.] Univ Washington, Dept Biostat, Seattle, WA 98195 USA.
[Psaty, Bruce M.] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Univ Washington, Dept Epidemiol, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Univ Washington, Dept Hlth Serv, Seattle, WA 98101 USA.
[Psaty, Bruce M.] Grp Hlth Res Inst, Grp Hlth Cooperat, Seattle, WA 98101 USA.
[Starr, J. M.] Alzheimer Scotland Res Ctr, Edinburgh EH8 9JZ, Midlothian, Scotland.
[Hayward, Caroline] Univ Edinburgh, MRC Human Genet Unit, Inst Genet & Mol Med, Edinburgh EH4 2XU, Midlothian, Scotland.
[Greinacher, Andreas; Thiele, Thomas] Univ Med Greifswald, Inst Immunol & Transfus Med, D-17475 Greifswald, Germany.
[Volzke, Henry] Univ Med Greifswald, Inst Community Med, D-13347 Greifswald, Germany.
[Uitterlinden, Andre G.] Erasmus MC, Dept Internal Med, NL-3000 Rotterdam, Netherlands.
[Uitterlinden, Andre G.] Netherlands Consortium Hlth Ageing, NL-3015 Rotterdam, Netherlands.
[Ganesh, Santhi K.] Univ Michigan, Dept Internal & Human Genet, Ann Arbor, MI 48108 USA.
[Faraday, Nauder] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21205 USA.
[Auer, Paul L.] Univ Wisconsin, Zilber Sch Publ Hlth, Milwaukee, WI 53205 USA.
[Reiner, Alex P.] Univ Washington, Dept Epidemiol, Seattle, WA 98105 USA.
[Eicher, John D.; Chami, Nathalie; Kacprowski, Tim; Nomura, Akihiro; Faraday, Nauder; Auer, Paul L.; Reiner, Alex P.; Lettre, Guillaume; Johnson, Andrew D.] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA.
RP Johnson, AD (reprint author), NHLBI, Populat Sci Branch, Framingham Heart Study, Framingham, MA 01702 USA.; Johnson, AD (reprint author), Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, Seattle, WA 98109 USA.
EM johnsonad2@nhlbi.nih.gov
RI Schurmann, Claudia/L-1204-2016; Floyd, James/G-7563-2015; Rioux,
John/A-9599-2015; Johnson, Andrew/G-6520-2013; Kacprowski,
Tim/K-8650-2013; Deloukas, Panos/B-2922-2013; Dube,
Marie-Pierre/B-9364-2008;
OI Evangelou, Evangelos/0000-0002-5488-2999; Dehghan,
Abbas/0000-0001-6403-016X; Schurmann, Claudia/0000-0003-4158-9192;
Rioux, John/0000-0001-7560-8326; Kacprowski, Tim/0000-0002-5393-2413;
Deloukas, Panos/0000-0001-9251-070X; Dube,
Marie-Pierre/0000-0001-8442-4393; Manichaikul, Ani/0000-0002-5998-795X;
Tajuddin, Salman M./0000-0002-7919-8528; Zonderman, Alan
B/0000-0002-6523-4778
FU British Heart Foundation [RG/14/5/30893]; Medical Research Council
[MC_PC_U127561128, MR/K026992/1]; NCATS NIH HHS [UL1 TR000124, UL1
TR000445]; NHGRI NIH HHS [U01 HG004402, U01 HG004603]; NHLBI NIH HHS
[HHSN268201100005C, HHSN268201100006C, HHSN268201100007C,
HHSN268201100008C, HHSN268201100009C, HHSN268201100010C,
HHSN268201100011C, HHSN268201100012C, HHSN268201100037C,
HHSN268201300026C, HHSN268201300028C, N01 HC025195, N01 HC095159, N01
HC095160, R01 HL059367, R01 HL086694, R01 HL087641, R01 HL087698, R01
HL112064, R01 HL122684, R01 HL127564, RC2 HL102419, U01 HL072518]; NICHD
NIH HHS [R01 HD074711]; NIDDK NIH HHS [P30 DK063491]
NR 77
TC 4
Z9 4
U1 3
U2 6
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0002-9297
EI 1537-6605
J9 AM J HUM GENET
JI Am. J. Hum. Genet.
PD JUL 7
PY 2016
VL 99
IS 1
BP 40
EP 55
DI 10.1016/j.ajhg.2016.05.005
PG 16
WC Genetics & Heredity
SC Genetics & Heredity
GA DT6TI
UT WOS:000381616600004
PM 27346686
ER
PT J
AU Gigante, ED
Benaliouad, F
Zamora-Olivencia, V
Wise, RA
AF Gigante, Eduardo D.
Benaliouad, Faiza
Zamora-Olivencia, Veronica
Wise, Roy A.
TI Optogenetic Activation of a Lateral Hypothalamic-Ventral Tegmental
Drive-Reward Pathway
SO PLOS ONE
LA English
DT Article
ID MEDIAL FOREBRAIN-BUNDLE; BRAIN-STIMULATION REWARD; SELF-STIMULATION;
ELECTRICAL-STIMULATION; REFRACTORY PERIODS; PREOPTIC AREA; RAT;
DOPAMINE; SEEKING; FIBERS
AB Electrical stimulation of the lateral hypothalamus can motivate feeding or can serve as a reward in its own right. It remains unclear whether the same or independent but anatomically overlapping circuitries mediate the two effects. Electrical stimulation findings implicate medial forebrain bundle (MFB) fibers of passage in both effects, and optogenetic studies confirm a contribution from fibers originating in the lateral hypothalamic area and projecting to or through the ventral tegmental area. Here we report that optogenetic activation of ventral tegmental fibers from cells of origin in more anterior or posterior portions of the MFB failed to induce either reward or feeding. The feeding and reward induced by optogenetic activation of fibers from the lateral hypothalamic cells of origin were influenced similarly by variations in stimulation pulse width and pulse frequency, consistent with the hypothesis of a common substrate for the two effects. There were, however, several cases where feeding but not self-stimulation or self-stimulation but not feeding were induced, consistent with the hypothesis that distinct but anatomically overlapping systems mediate the two effects. Thus while optogenetic stimulation provides a more selective tool for characterizing the mechanisms of stimulation-induced feeding and reward, it does not yet resolve the question of common or independent substrates.
C1 [Gigante, Eduardo D.; Benaliouad, Faiza; Zamora-Olivencia, Veronica; Wise, Roy A.] NIDA, Intramural Res Program, NIH, Dept Hlth & Human Serv, Baltimore, MD 21224 USA.
RP Wise, RA (reprint author), NIDA, Intramural Res Program, NIH, Dept Hlth & Human Serv, Baltimore, MD 21224 USA.
EM rwise@intra.nida.nih.gov
FU Intramural Research Program of the National Institute on Drug Abuse,
National Institutes of Health
FX This study was funded by the Intramural Research Program of the National
Institute on Drug Abuse, National Institutes of Health.
NR 48
TC 0
Z9 0
U1 4
U2 4
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 7
PY 2016
VL 11
IS 7
AR e0158885
DI 10.1371/journal.pone.0158885
PG 13
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR3OC
UT WOS:000379811500058
PM 27387668
ER
PT J
AU Jones, K
Ballesteros, A
Mentink-Kane, M
Warren, J
Rattila, S
Malech, H
Kang, E
Dveksler, G
AF Jones, Karlie
Ballesteros, Angela
Mentink-Kane, Margaret
Warren, James
Rattila, Shemona
Malech, Harry
Kang, Elizabeth
Dveksler, Gabriela
TI PSG9 Stimulates Increase in FoxP3(+) Regulatory T-Cells through the
TGF-beta 1 Pathway
SO PLOS ONE
LA English
DT Article
ID LATENT TGF-BETA; PREGNANCY-SPECIFIC BETA-1-GLYCOPROTEIN;
GROWTH-FACTOR-BETA; TRANSCRIPTION FACTOR FOXP3; GLYCOPROTEIN 1 PSG1;
CUTTING EDGE; EXPRESSION; ACTIVATION; RECEPTOR; SURFACE
AB The pregnancy-specific glycoproteins (PSGs) are a family of proteins secreted by the syncytiotrophoblast of the placenta and are the most abundant trophoblastic proteins in maternal blood during the third trimester. The human PSG family consists of 10 protein-coding genes, some of which have a possible role in maintaining maternal immune tolerance to the fetus. PSG9 was reported as a potential predictive biomarker of pre-eclampsia, a serious complication of pregnancy that has been related to immunological dysfunction at the fetal-maternal interface. Therefore, we hypothesized that PSG9 may have an immunoregulatory role during pregnancy. We found that PSG9 binds to LAP and activates the latent form of TGF-beta 1. In addition, PSG9 induces the secretion of TGF-beta 1 from macrophages but not from CD4(+) T-cells. TGF-beta 1 is required for the ex vivo differentiation of regulatory T-cells and, consistent with the ability of PSG9 to activate this cytokine, we observed that PSG9 induces the differentiation of FoxP3(+) regulatory T-cells from naive murine and human T-cells. Cytokines that are associated with inflammatory responses were also reduced in the supernatants of T-cells treated with PSG9, suggesting that PSG9, through its activation of TGF beta-1, could be a potent inducer of immune tolerance.
C1 [Jones, Karlie; Malech, Harry; Kang, Elizabeth] NIAID, NIH, Bethesda, MD USA.
[Ballesteros, Angela] NIDCD, NIH, Bethesda, MD USA.
[Mentink-Kane, Margaret; Warren, James; Rattila, Shemona; Dveksler, Gabriela] USUHS, Dept Pathol, Bethesda, MD USA.
RP Dveksler, G (reprint author), USUHS, Dept Pathol, Bethesda, MD USA.
EM gabriela.dveksler@usuhs.edu
FU USUHS [R0742495]; Intramural Research Program of the National Institute
of Allergy and Infectious Diseases, National Institutes of Health (NIH)
[Z-A1-00-0989]
FX This work was supported by the USUHS intramural award R0742495 to GD and
the Intramural Research Program of the National Institute of Allergy and
Infectious Diseases, National Institutes of Health (NIH) under
intramural project number Z-A1-00-0989 supported the research reported
in this publication. The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the
manuscript.
NR 52
TC 1
Z9 1
U1 0
U2 2
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 7
PY 2016
VL 11
IS 7
AR e0158050
DI 10.1371/journal.pone.0158050
PG 18
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR3OC
UT WOS:000379811500012
PM 27389696
ER
PT J
AU Curtis, LM
Pavletic, SZ
AF Curtis, Lauren M.
Pavletic, Steven Z.
TI IL-2, the next best thing in chronic GVHD therapy?
SO BLOOD
LA English
DT Editorial Material
ID VERSUS-HOST-DISEASE
C1 [Curtis, Lauren M.; Pavletic, Steven Z.] NCI, Bethesda, MD 20892 USA.
RP Curtis, LM (reprint author), NCI, Bethesda, MD 20892 USA.
NR 8
TC 0
Z9 0
U1 1
U2 1
PU AMER SOC HEMATOLOGY
PI WASHINGTON
PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA
SN 0006-4971
EI 1528-0020
J9 BLOOD
JI Blood
PD JUL 7
PY 2016
VL 128
IS 1
BP 13
EP 15
DI 10.1182/blood-2016-05-711796
PG 5
WC Hematology
SC Hematology
GA DQ5MN
UT WOS:000379249600007
PM 27389542
ER
PT J
AU Saba, NS
Liu, DL
Herman, SEM
Underbayev, C
Tian, X
Behrend, D
Weniger, MA
Skarzynski, M
Gyamfi, J
Fontan, L
Melnick, A
Grant, C
Roschewski, M
Navarro, A
Bea, S
Pittaluga, S
Dunleavy, K
Wilson, WH
Wiestner, A
AF Saba, Nakhle S.
Liu, Delong
Herman, Sarah E. M.
Underbayev, Chingiz
Tian, Xin
Behrend, David
Weniger, Marc A.
Skarzynski, Martin
Gyamfi, Jennifer
Fontan, Lorena
Melnick, Ari
Grant, Cliona
Roschewski, Mark
Navarro, Alba
Bea, Silvia
Pittaluga, Stefania
Dunleavy, Kieron
Wilson, Wyndham H.
Wiestner, Adrian
TI Pathogenic role of B-cell receptor signaling and canonical NF-kappa B
activation in mantle cell lymphoma
SO BLOOD
LA English
DT Article
ID CHRONIC LYMPHOCYTIC-LEUKEMIA; NON-HODGKIN-LYMPHOMA; SMALL-MOLECULE
INHIBITORS; WALDENSTROMS MACROGLOBULINEMIA; TARGETING BTK; IBRUTINIB;
EXPRESSION; THERAPY; KINASE; SOX11
AB To interrogate signaling pathways activated in mantle cell lymphoma (MCL) in vivo, we contrasted gene expression profiles of 55 tumor samples isolated from blood and lymph nodes from 43 previously untreated patients with active disease. In addition to lymph nodes, MCL often involves blood, bone marrow, and spleen and is incurable for most patients. Recently, the Bruton tyrosine kinase (BTK) inhibitor ibrutinib demonstrated important clinical activity in MCL. However, the role of specific signaling pathways in the lymphomagenesis of MCL and the biologic basis for ibrutinib sensitivity of these tumors are unknown. Here, we demonstrate activation of B-cell receptor (BCR) and canonical NF-kappa B signaling specifically in MCL cells in the lymph node. Quantification of BCR signaling strength, reflected in the expression of BCR regulated genes, identified a subset of patients with inferior survival after cytotoxic therapy. Tumor proliferation was highest in the lymph node and correlated with the degree of BCR activation. A subset of leukemic tumors showed active BCR and NF-kB signaling apparently independent of microenvironmental support. In one of these samples, we identified a novel somatic mutation in RELA (E39Q). This sample was resistant to ibrutinib-mediated inhibition of NF-kappa B and apoptosis. In addition, we identified germ line variants in genes encoding regulators of the BCR and NF-kB pathway previously implicated in lymphomagenesis. In conclusion, BCR signaling, activated in the lymph node microenvironment in vivo, appears to promote tumor proliferation and survival and may explain the sensitivity of this lymphoma to BTK inhibitors.
C1 [Saba, Nakhle S.; Liu, Delong; Herman, Sarah E. M.; Underbayev, Chingiz; Behrend, David; Weniger, Marc A.; Skarzynski, Martin; Gyamfi, Jennifer; Wiestner, Adrian] NHLBI, Hematol Branch, NIH, Bld 10,CRC 3-5140,10 Ctr Dr, Bethesda, MD 20892 USA.
[Saba, Nakhle S.] Tulane Univ, Dept Med, Sect Hematol & Med Oncol, New Orleans, LA 70118 USA.
[Tian, Xin] NHLBI, Off Biostat Res, NIH, Bld 10,CRC 3-5140,10 Ctr Dr, Bethesda, MD 20892 USA.
[Fontan, Lorena; Melnick, Ari] Weill Cornell Med Coll, Dept Med, Div Hematol & Med Oncol, New York, NY USA.
[Grant, Cliona; Roschewski, Mark; Wilson, Wyndham H.] NCI, Lymphoid Malignancies Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
[Navarro, Alba; Bea, Silvia] Inst Invest Biomed August Pi & Sunyer, Barcelona, Spain.
[Pittaluga, Stefania] NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA.
RP Wiestner, A (reprint author), NHLBI, Hematol Branch, NIH, Bld 10,CRC 3-5140,10 Ctr Dr, Bethesda, MD 20892 USA.
EM wiestnera@mail.nih.gov
RI Navarro, Alba/H-2611-2015
OI Navarro, Alba/0000-0002-4041-0974
FU Intramural Research Programs of the National Heart, Lung, and Blood
Institute; National Cancer Institute, National Institutes of Health
FX This work was funded by the Intramural Research Programs of the National
Heart, Lung, and Blood Institute and the National Cancer Institute,
National Institutes of Health.
NR 45
TC 5
Z9 5
U1 3
U2 9
PU AMER SOC HEMATOLOGY
PI WASHINGTON
PA 2021 L ST NW, SUITE 900, WASHINGTON, DC 20036 USA
SN 0006-4971
EI 1528-0020
J9 BLOOD
JI Blood
PD JUL 7
PY 2016
VL 128
IS 1
BP 82
EP 92
DI 10.1182/blood-2015-11-681460
PG 11
WC Hematology
SC Hematology
GA DQ5MN
UT WOS:000379249600016
PM 27127301
ER
PT J
AU Torres-Salgado, JF
Comas-Garcia, M
Villagrana-Escareno, MV
Duran-Meza, AL
Ruiz-Garcia, J
Cadena-Nava, RD
AF Torres-Salgado, Jose F.
Comas-Garcia, Mauricio
Villagrana-Escareno, Maria V.
Duran-Meza, Ana L.
Ruiz-Garcia, Jaime
Cadena-Nava, Ruben D.
TI Physicochemical Study of Viral Nanoparticles at the Air/Water Interface
SO JOURNAL OF PHYSICAL CHEMISTRY B
LA English
DT Article
ID LANGMUIR-BLODGETT-FILMS; GOLD NANOPARTICLES; PROTEIN ADSORPTION;
COLLOIDAL CRYSTALS; CARBON NANOTUBES; CAPSID PROTEIN; PHASE-DIAGRAM;
AIR-WATER; MONOLAYERS; PARTICLES
AB The assembly of most single-stranded RNA (ssRNA) viruses into icosahedral nucleocapsids is a spontaneous process driven by protein protein and RNA protein interactions. The precise nature of these interactions results in the assembly of extremely monodisperse and structurally indistinguishable nucleocapsids. In this work, by using a ssRNA plant virus (cowpea chlorotic mottle virus [CCMV]) as a charged nanoparticle we show that the diffusion of these nanoparticles from the bulk solution to the air/water interface is an irreversible adsorption process. By using the Langmuir technique, we measured the diffusion and adsorption of viral nucleocapsids at the air/water interface at different pH conditions. The pH changes, and therefore in the net surface charge of the virions, have a great influence in the diffusion rate from the bulk solution to the air/water interface. Moreover, assembly of mesoscopic and microscopic viral aggregates at this interface depends on the net surface charge of the virions and the surface pressure. By using Brewster's angle microscopy we characterized these structures at the interface. Most common structures observed were clusters of virions and soap-frothlike micron-size structures. Furthermore, the CCMV films were compressed to form monolayers and multilayers from moderate to high surface pressures, respectively. After transferring the films from the air/water interface onto mica by using the Langmuir-Blodgett technique, their morphology was characterized by atomic force microscopy. These viral monolayers showed closed-packing nano- and microscopic arrangements.
C1 [Torres-Salgado, Jose F.; Villagrana-Escareno, Maria V.; Duran-Meza, Ana L.; Ruiz-Garcia, Jaime] Univ Autonoma San Luis Potosi, Inst Fis, Alvaro Obregon 64, San Luis Potosi 78000, San Luis Potosi, Mexico.
[Comas-Garcia, Mauricio] NCI, HIV Dynam & Replicat Program, Frederick, MD 21702 USA.
[Cadena-Nava, Ruben D.] Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Carretera Tijuana Ensenada Km 107, Ensenada 22860, Baja California, Mexico.
RP Ruiz-Garcia, J (reprint author), Univ Autonoma San Luis Potosi, Inst Fis, Alvaro Obregon 64, San Luis Potosi 78000, San Luis Potosi, Mexico.; Cadena-Nava, RD (reprint author), Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol, Carretera Tijuana Ensenada Km 107, Ensenada 22860, Baja California, Mexico.
EM jaime@dec1.ifisica.uaslp.mx; rcadena74@gmail.com
OI Comas-Garcia, Mauricio/0000-0002-7733-5138
FU Consejo Nacional de Ciencia y Tecnologia (CONACYT) [237439, 239878,
225019, 248787]
FX We would like to acknowledge the support from the Consejo Nacional de
Ciencia y Tecnologia (CONACYT) Grants 237439, 239878, 225019, and
248787.
NR 48
TC 0
Z9 0
U1 9
U2 15
PU AMER CHEMICAL SOC
PI WASHINGTON
PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN 1520-6106
J9 J PHYS CHEM B
JI J. Phys. Chem. B
PD JUL 7
PY 2016
VL 120
IS 26
BP 5864
EP 5873
DI 10.1021/acs.jpcb.6b00624
PG 10
WC Chemistry, Physical
SC Chemistry
GA DQ8JN
UT WOS:000379457200010
PM 26999022
ER
PT J
AU Berezhkovskii, AM
Szabo, A
AF Berezhkovskii, Alexander M.
Szabo, Attila
TI Theory of Crowding Effects on Bimolecular Reaction Rates
SO JOURNAL OF PHYSICAL CHEMISTRY B
LA English
DT Article
ID PHYSIOLOGICAL CONSEQUENCES; DIFFUSION; CONFINEMENT; CYTOPLASM; BINDING;
VOLUME
AB An analytical expression for the rate constant of a diffusion-influenced bimolecular reaction in a crowded environment is derived in the framework of a microscopic model that accounts for: (1) the slowdown of diffusion due to crowding and the dependence of the diffusivity on the distance between the reactants, (2) a crowding induced attractive short-range potential of mean force, and (3) nonspecific reversible binding to the crowders. This expression spans the range from reaction to diffusion control. Crowding can increase the reaction-controlled rate by inducing an effective attraction between reactants but decrease the diffusion-controlled rate by reducing their relative diffusivity.
C1 [Berezhkovskii, Alexander M.] NIH, Math & Stat Comp Lab, Div Computat Biosci, Ctr Informat Technol, Bldg 10, Bethesda, MD 20892 USA.
[Szabo, Attila] Natl Inst Diabet & Digest & Kidney Dis, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
RP Szabo, A (reprint author), Natl Inst Diabet & Digest & Kidney Dis, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
EM attilas@niddk.nih.gov
RI Szabo, Attila/H-3867-2012
FU Intramural Research Program of the NIH, Center for Information
Technology; National Institute of Diabetes and Digestive and Kidney
Diseases
FX We are grateful to Allen Minton for helpful discussions. This study was
supported by the Intramural Research Program of the NIH, Center for
Information Technology and National Institute of Diabetes and Digestive
and Kidney Diseases.
NR 26
TC 0
Z9 0
U1 7
U2 11
PU AMER CHEMICAL SOC
PI WASHINGTON
PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
SN 1520-6106
J9 J PHYS CHEM B
JI J. Phys. Chem. B
PD JUL 7
PY 2016
VL 120
IS 26
BP 5998
EP 6002
DI 10.1021/acs.jpcb.6b01892
PG 5
WC Chemistry, Physical
SC Chemistry
GA DQ8JN
UT WOS:000379457200025
PM 27096470
ER
PT J
AU Behrens, T
Gross, I
Siemiatycki, J
Conway, DI
Olsson, A
Stucker, I
Guida, F
Jockel, KH
Pohlabeln, H
Ahrens, W
Bruske, I
Wichmann, HE
Gustavsson, P
Consonni, D
Merletti, F
Richiardi, L
Simonato, L
Fortes, C
Parent, ME
McLaughlin, J
Demers, P
Landi, MT
Caporaso, N
Zaridze, D
Szeszenia-Dabrowska, N
Rudnai, P
Lissowska, J
Fabianova, E
Tardon, A
Field, JK
Dumitru, RS
Bencko, V
Foretova, L
Janout, V
Kromhout, H
Vermeulen, R
Boffetta, P
Straif, K
Schuz, J
Hovanec, J
Kendzia, B
Pesch, B
Bruning, T
AF Behrens, Thomas
Gross, Isabelle
Siemiatycki, Jack
Conway, David I.
Olsson, Ann
Stuecker, Isabelle
Guida, Florence
Joeckel, Karl-Heinz
Pohlabeln, Hermann
Ahrens, Wolfgang
Brueske, Irene
Wichmann, Heinz-Erich
Gustavsson, Per
Consonni, Dario
Merletti, Franco
Richiardi, Lorenzo
Simonato, Lorenzo
Fortes, Cristina
Parent, Marie-Elise
McLaughlin, John
Demers, Paul
Landi, Maria Teresa
Caporaso, Neil
Zaridze, David
Szeszenia-Dabrowska, Neonila
Rudnai, Peter
Lissowska, Jolanta
Fabianova, Eleonora
Tardon, Adonina
Field, John K.
Dumitru, Rodica Stanescu
Bencko, Vladimir
Foretova, Lenka
Janout, Vladimir
Kromhout, Hans
Vermeulen, Roel
Boffetta, Paolo
Straif, Kurt
Schuez, Joachim
Hovanec, Jan
Kendzia, Benjamin
Pesch, Beate
Bruening, Thomas
TI Occupational prestige, social mobility and the association with lung
cancer in men
SO BMC CANCER
LA English
DT Article
DE Life course; Occupational history; Social prestige; Socio-economic
status; SYNERGY; Transitions
ID SOCIOECONOMIC-STATUS; EDUCATIONAL INEQUALITIES; HEALTH RESEARCH;
LIFE-COURSE; RISK; MORTALITY; SMOKING; INDICATORS; POSITION; US
AB Background: The nature of the association between occupational social prestige, social mobility, and risk of lung cancer remains uncertain. Using data from the international pooled SYNERGY case-control study, we studied the association between lung cancer and the level of time-weighted average occupational social prestige as well as its lifetime trajectory.
Methods: We included 11,433 male cases and 14,147 male control subjects. Each job was translated into an occupational social prestige score by applying Treiman's Standard International Occupational Prestige Scale (SIOPS). SIOPS scores were categorized as low, medium, and high prestige (reference). We calculated odds ratios (OR) with 95 % confidence intervals (CI), adjusting for study center, age, smoking, ever employment in a job with known lung carcinogen exposure, and education. Trajectories in SIOPS categories from first to last and first to longest job were defined as consistent, downward, or upward. We conducted several subgroup and sensitivity analyses to assess the robustness of our results.
Results: We observed increased lung cancer risk estimates for men with medium (OR = 1.23; 95 % CI 1.13-1.33) and low occupational prestige (OR = 1.44; 95 % CI 1.32-1.57). Although adjustment for smoking and education reduced the associations between occupational prestige and lung cancer, they did not explain the association entirely. Traditional occupational exposures reduced the associations only slightly. We observed small associations with downward prestige trajectories, with ORs of 1.13, 95 % CI 0.88-1.46 for high to low, and 1.24; 95 % CI 1.08-1.41 for medium to low trajectories.
Conclusions: Our results indicate that occupational prestige is independently associated with lung cancer among men.
C1 [Behrens, Thomas; Gross, Isabelle; Hovanec, Jan; Kendzia, Benjamin; Pesch, Beate; Bruening, Thomas] Inst Ruhr Univ Bochum, Inst Prevent & Occupat Med German Social Accident, Brkle de La Camp Pl 1, D-44789 Bochum, Germany.
[Siemiatycki, Jack] Univ Montreal, Hosp Res Ctr CRCHUM, Montreal, PQ, Canada.
[Siemiatycki, Jack] Univ Montreal, Sch Publ Hlth, Montreal, PQ, Canada.
[Conway, David I.] Univ Glasgow, Sch Dent, Coll Med Vet & Life Sci, Glasgow G2 3JZ, Lanark, Scotland.
[Olsson, Ann; Straif, Kurt; Schuez, Joachim] IARC, Lyon, France.
[Olsson, Ann] Karolinska Inst, Inst Environm Med, Stockholm, Sweden.
[Stuecker, Isabelle; Guida, Florence] INSERM, Ctr Res Epidemiol & Populat Hlth CESP, F-94807 Villejuif, France.
[Stuecker, Isabelle; Guida, Florence] Univ Paris 11, UMRS 1018, F-94807 Villejuif, France.
[Joeckel, Karl-Heinz] Univ Hosp Essen, Inst Med Informat Biometry & Epidemiol, Essen, Germany.
[Pohlabeln, Hermann; Ahrens, Wolfgang] Leibniz Inst Prevent Res & Epidemiol BIPS GmbH, Bremen, Germany.
[Ahrens, Wolfgang] Univ Bremen, Inst Stat, Bremen, Germany.
[Brueske, Irene; Wichmann, Heinz-Erich] Helmholtz Zentrum Munchen, Inst Epidemiol, Neuherberg, Germany.
[Consonni, Dario] Fdn IRCCS Ca Granda Osped Maggiore Policlin, Milan, Italy.
[Merletti, Franco; Richiardi, Lorenzo] Univ Turin, Dept Med Sci, Canc Epidemiol Unit, Turin, Italy.
[Simonato, Lorenzo] Univ Padua, Dept Mol Med, Lab Publ Hlth & Populat Studies, Padua, Italy.
[Fortes, Cristina] Ist Dermopatico Immacolata, Epidemiol Unit, Rome, Italy.
[Parent, Marie-Elise] Univ Quebec, INRS Inst Armand Frappier, Laval, PQ, Canada.
[McLaughlin, John] Occupat Canc Res Ctr, Canc Care Ontario, Toronto, ON, Canada.
[Landi, Maria Teresa; Caporaso, Neil] NCI, Div Canc Epidemiol & Genet, Bethesda, MD USA.
[Zaridze, David] Russian Canc Res Ctr, Inst Carcinogenesis, Moscow, Russia.
[Szeszenia-Dabrowska, Neonila] Nofer Inst Occupat Med, Lodz, Poland.
[Rudnai, Peter] Natl Ctr Publ Hlth, Budapest, Hungary.
[Lissowska, Jolanta] M Sklodowska Curie Canc Ctr, Warsaw, Poland.
[Lissowska, Jolanta] Inst Oncol, Warsaw, Poland.
[Fabianova, Eleonora] Prevent Occupat Med, Reg Author Publ Hlth, Banska Bystrica, Slovakia.
[Tardon, Adonina] Univ Oviedo Ciber Epidemiol, CIBERESP, Mol Epidemiol Canc Unit, Oviedo, Spain.
[Field, John K.] Univ Liverpool, Canc Res Ctr, Roy Castle Lung Canc Res Programme, Liverpool, Merseyside, England.
[Field, John K.] Univ Liverpool, Inst Translat Med, Dept Mol & Clin Canc Med, Liverpool, Merseyside, England.
[Dumitru, Rodica Stanescu] Natl Inst Publ Hlth, Bucharest, Romania.
[Bencko, Vladimir] Charles Univ Prague, Fac Med 1, Inst Hyg & Epidemiol, Prague, Czech Republic.
[Foretova, Lenka] Masaryk Univ, Masaryk Mem Canc Inst, Dept Canc Epidemiol & Genet, Brno, Czech Republic.
[Foretova, Lenka] Masaryk Univ, Fac Med, Brno, Czech Republic.
[Janout, Vladimir] Palacky Univ, Fac Med, Olomouc, Czech Republic.
[Kromhout, Hans; Vermeulen, Roel] Univ Utrecht, Inst Risk Assessment Sci, Environm Epidemiol Div, Utrecht, Netherlands.
[Boffetta, Paolo] Icahn Sch Med Mt Sinai, Inst Canc, New York, NY USA.
[Wichmann, Heinz-Erich] Icahn Sch Med Mt Sinai, Inst Translat Epidemiol, New York, NY USA.
[Janout, Vladimir] Tech Univ Munich, Inst Med Stat & Epidemiol, Munich, Germany.
Univ Ostrava, Fac Med, Ostrava, Czech Republic.
RP Behrens, T (reprint author), Inst Ruhr Univ Bochum, Inst Prevent & Occupat Med German Social Accident, Brkle de La Camp Pl 1, D-44789 Bochum, Germany.
EM behrens@ipa-dguv.de
RI Consonni, Dario/K-7943-2016; Bruning, Thomas/G-8120-2015; Vermeulen,
Roel/F-8037-2011;
OI Consonni, Dario/0000-0002-8935-3843; Bruning,
Thomas/0000-0001-9560-5464; Vermeulen, Roel/0000-0003-4082-8163; Ahrens,
Wolfgang/0000-0003-3777-570X
FU German Social Accident Insurance [FP 271]; Canadian Institutes of Health
Research and Guzzo-SRC Chair in Environment and Cancer; Fondation de
France; German Federal Ministry of Education, Science, Research, and
Technology; Ministry of Labour and Social Affairs; EC's INCO-COPERNICUS
Program; Polish State Committee for Science Research; Roy Castle
Foundation; NIH/NCI/DCEG Intramural Research Program; Lombardy Region;
INAIL; European Union Nuclear Fission Safety Program; Italian
Association for Cancer Research; Region Piedmont; Compagnia di San
Paolo; Europe Against Cancer Program; Swedish Council for Work Life
Research; Swedish EPA; University of Oviedo; European Regional
Development Fund; State Budget of the Czech Republic (RECAMO)
[CZ.1.05/2.1.00/03.0101]; CIBERESP; [FISS-PI060604]
FX This work was supported by the German Social Accident Insurance (grant
number FP 271). Grant sponsors of the individual studies were the
Canadian Institutes of Health Research and Guzzo-SRC Chair in
Environment and Cancer; the Fondation de France; the German Federal
Ministry of Education, Science, Research, and Technology and the
Ministry of Labour and Social Affairs; EC's INCO-COPERNICUS Program;
Polish State Committee for Science Research; Roy Castle Foundation;
NIH/NCI/DCEG Intramural Research Program; Lombardy Region; INAIL and the
European Union Nuclear Fission Safety Program; Italian Association for
Cancer Research; Region Piedmont; Compagnia di San Paolo; Europe Against
Cancer Program, the Swedish Council for Work Life Research and the
Swedish EPA; the University of Oviedo; the European Regional Development
Fund and the State Budget of the Czech Republic (RECAMO,
CZ.1.05/2.1.00/03.0101); CIBERESP; and FISS-PI060604.
NR 37
TC 0
Z9 0
U1 9
U2 10
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1471-2407
J9 BMC CANCER
JI BMC Cancer
PD JUL 7
PY 2016
VL 16
AR 395
DI 10.1186/s12885-016-2432-9
PG 12
WC Oncology
SC Oncology
GA DQ3TB
UT WOS:000379124600001
PM 27388894
ER
PT J
AU Ding, HY
Smith, RG
Poleg-Polsky, A
Diamond, JS
Briggman, KL
AF Ding, Huayu
Smith, Robert G.
Poleg-Polsky, Alon
Diamond, Jeffrey S.
Briggman, Kevin L.
TI Species-specific wiring for direction selectivity in the mammalian
retina
SO NATURE
LA English
DT Article
ID STARBURST AMACRINE CELLS; RABBIT RETINA; MOUSE RETINA; GANGLION-CELLS;
NEURAL COMPUTATION; SYNAPTIC INPUT; AXIAL LENGTH; DENDRITES; CIRCUIT;
MOTION
AB Directionally tuned signalling in starburst amacrine cell (SAC) dendrites lies at the heart of the circuit that detects the direction of moving stimuli in the mammalian retina. The relative contributions of intrinsic cellular properties and network connectivity to SAC direction selectivity remain unclear. Here we present a detailed connectomic reconstruction of SAC circuitry in mouse retina and describe two previously unknown features of synapse distributions along SAC dendrites: input and output synapses are segregated, with inputs restricted to proximal dendrites; and the distribution of inhibitory inputs is fundamentally different from that observed in rabbit retina. An anatomically constrained SAC network model suggests that SAC-SAC wiring differences between mouse and rabbit retina underlie distinct contributions of synaptic inhibition to velocity and contrast tuning and receptive field structure. In particular, the model indicates that mouse connectivity enables SACs to encode lower linear velocities that account for smaller eye diameter, thereby conserving angular velocity tuning. These predictions are confirmed with calcium imaging of mouse SAC dendrites responding to directional stimuli.
C1 [Ding, Huayu; Poleg-Polsky, Alon; Diamond, Jeffrey S.] NINDS, Synapt Physiol Sect, Bethesda, MD 20892 USA.
[Smith, Robert G.] Univ Penn, Dept Neurosci, Philadelphia, PA 19104 USA.
[Briggman, Kevin L.] Max Planck Inst Med Res, Dept Biomed Opt, Jahnstr 29, D-69120 Heidelberg, Germany.
[Briggman, Kevin L.] NINDS, Circuit Dynam & Connect Unit, Bethesda, MD 20892 USA.
RP Briggman, KL (reprint author), Max Planck Inst Med Res, Dept Biomed Opt, Jahnstr 29, D-69120 Heidelberg, Germany.; Briggman, KL (reprint author), NINDS, Circuit Dynam & Connect Unit, Bethesda, MD 20892 USA.
EM kevin.briggman@nih.gov
RI Diamond, Jeffrey/C-1835-2015
OI Diamond, Jeffrey/0000-0002-1770-2629
FU NIH [EY016607, EY022070]; NINDS [NS003145, NS003133]; Max-Planck
Society; Pew Charitable Trusts
FX We thank W. Denk for supporting the collection of the serial block-face
scanning electron microscopy data in his laboratory. This work was
supported by NIH grants EY016607 and EY022070 (RGS), by the NINDS
Intramural Research Program (NS003145; J.S.D.) and (NS003133; K.L.B.),
the Max-Planck Society (K.L.B.), and the Pew Charitable Trusts (K.L.B.).
NR 47
TC 7
Z9 7
U1 6
U2 11
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 0028-0836
EI 1476-4687
J9 NATURE
JI Nature
PD JUL 7
PY 2016
VL 535
IS 7610
BP 105
EP +
DI 10.1038/nature18609
PG 17
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ2EU
UT WOS:000379015600033
PM 27350241
ER
PT J
AU Zhang, R
Miner, JJ
Gorman, MJ
Rausch, K
Ramage, H
White, JP
Zuiani, A
Zhang, P
Fernandez, E
Zhang, Q
Dowd, KA
Pierson, TC
Cherry, S
Diamond, MS
AF Zhang, Rong
Miner, Jonathan J.
Gorman, Matthew J.
Rausch, Keiko
Ramage, Holly
White, James P.
Zuiani, Adam
Zhang, Ping
Fernandez, Estefania
Zhang, Qiang
Dowd, Kimberly A.
Pierson, Theodore C.
Cherry, Sara
Diamond, Michael S.
TI A CRISPR screen defines a signal peptide processing pathway required by
flaviviruses
SO NATURE
LA English
DT Article
ID WEST-NILE-VIRUS; BORNE ENCEPHALITIS-VIRUS; HEPATITIS-C VIRUS;
MONOCLONAL-ANTIBODIES; ENVELOPE PROTEIN; HUMAN-CELLS; INFECTION; GENES;
NEUTRALIZATION; IDENTIFICATION
AB Flaviviruses infect hundreds of millions of people annually, and no antiviral therapy is available(1,2). We performed a genome-wide CRISPR/Cas9-based screen to identify host genes that, when edited, resulted in reduced flavivirus infection. Here, we validated nine human genes required for flavivirus infectivity, and these were associated with endoplasmic reticulum functions including translocation, protein degradation, and N-linked glycosylation. In particular, a subset of endoplasmic reticulum-associated signal peptidase complex (SPCS) proteins was necessary for proper cleavage of the flavivirus structural proteins (prM and E) and secretion of viral particles. Loss of SPCS1 expression resulted in markedly reduced yield of all Flaviviridae family members tested (West Nile, dengue, Zika, yellow fever, Japanese encephalitis, and hepatitis C viruses), but had little impact on alphavirus, bunyavirus, or rhabdovirus infection or the surface expression or secretion of diverse host proteins. We found that SPCS1 dependence could be bypassed by replacing the native prM protein leader sequences with a class I major histocompatibility complex (MHC) antigen leader sequence. Thus, SPCS1, either directly or indirectly via its interactions with host proteins, preferentially promotes the processing of specific protein cargo, and Flaviviridae have a unique dependence on this signal peptide processing pathway. SPCS1 and other signal processing pathway members could represent pharmacological targets for inhibiting infection by the expanding number of flaviviruses of medical concern.
C1 [Zhang, Rong; Miner, Jonathan J.; Gorman, Matthew J.; White, James P.; Zuiani, Adam; Zhang, Ping; Fernandez, Estefania; Zhang, Qiang; Diamond, Michael S.] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA.
[Rausch, Keiko; Ramage, Holly; Cherry, Sara] Univ Penn, Dept Microbiol, Perelman Sch Med, Philadelphia, PA 19104 USA.
[Zhang, Ping] Sun Yat Sen Univ, Inst Human Virol, Zhongshan Sch Med, Dept Immunol, Guangzhou 510080, Guangdong, Peoples R China.
[Dowd, Kimberly A.; Pierson, Theodore C.] NIAID, Viral Pathogenesis Sect, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Diamond, Michael S.] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA.
[Diamond, Michael S.] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA.
[Diamond, Michael S.] Washington Univ, Sch Med, Ctr Human Immunol & Immunotherapy Programs, St Louis, MO 63110 USA.
RP Diamond, MS (reprint author), Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA.; Diamond, MS (reprint author), Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA.; Diamond, MS (reprint author), Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA.; Diamond, MS (reprint author), Washington Univ, Sch Med, Ctr Human Immunol & Immunotherapy Programs, St Louis, MO 63110 USA.
EM diamond@borcim.wustl.edu
FU NIH [U19 AI083019, U19 AI106772, R01 AI104972, T32 AI007163]; Washington
University Institute of Clinical and Translational Sciences from the
National Center for Advancing Translational Sciences [UL1 TR000448];
Washington University Institute of Clinical and Translational Sciences
from the National Institute of General Medical Sciences [P41
GM103422-35]; NIAID
FX This work was supported by NIH grants U19 AI083019 (M.S.D.), U19
AI106772 (M.S.D.), R01 AI104972 (M.S.D.), and T32 AI007163 (E.F.) and by
the Washington University Institute of Clinical and Translational
Sciences (UL1 TR000448 from the National Center for Advancing
Translational Sciences and P41 GM103422-35 from the National Institute
of General Medical Sciences). T.C.P and K.A.D are supported by the
intramural program of NIAID. We thank R. Kuhn, A. Garcia-Sastre, H.
Zhao, D. Fremont, X. Wang, and R. Townsend for reagents, experimental
advice, and data analysis; P. Erdmann-Gilmore, R. Connors, Y. Mi, and H.
Lin for expert technical assistance; and X. de Lamballerie and the
European Virus Archive goes Global (EVAg) for consenting to the use of
H/PF/2013 ZIKV strain for this study under a material transfer agreement
with the EVAg parter, Aix-Marseille Universite.
NR 49
TC 23
Z9 23
U1 28
U2 69
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 0028-0836
EI 1476-4687
J9 NATURE
JI Nature
PD JUL 7
PY 2016
VL 535
IS 7610
BP 164
EP +
DI 10.1038/nature18625
PG 19
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ2EU
UT WOS:000379015600045
PM 27383988
ER
PT J
AU Boyden, SE
Metcalfe, DD
Komarow, HD
AF Boyden, Steven E.
Metcalfe, Dean D.
Komarow, Hirsh D.
TI Vibratory Urticaria and ADGRE2 REPLY
SO NEW ENGLAND JOURNAL OF MEDICINE
LA English
DT Letter
ID PHYSICAL HYPERSENSITIVITY; ANGIOEDEMA
C1 [Boyden, Steven E.] NHGRI, Bethesda, MD 20892 USA.
[Metcalfe, Dean D.; Komarow, Hirsh D.] NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA.
RP Komarow, HD (reprint author), NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA.
EM komarowh@mail.nih.gov
NR 3
TC 0
Z9 0
U1 0
U2 0
PU MASSACHUSETTS MEDICAL SOC
PI WALTHAM
PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA
SN 0028-4793
EI 1533-4406
J9 NEW ENGL J MED
JI N. Engl. J. Med.
PD JUL 7
PY 2016
VL 375
IS 1
BP 95
EP 95
PG 1
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ1ZP
UT WOS:000379000200024
PM 27406365
ER
PT J
AU Volkow, ND
McLellan, AT
AF Volkow, Nora D.
McLellan, A. Thomas
TI Mitigation Strategies for Opioid Abuse REPLY
SO NEW ENGLAND JOURNAL OF MEDICINE
LA English
DT Letter
C1 [Volkow, Nora D.] NIH, Bldg 10, Bethesda, MD 20892 USA.
[McLellan, A. Thomas] Treatment Res Inst, Philadelphia, PA USA.
RP Volkow, ND (reprint author), NIH, Bldg 10, Bethesda, MD 20892 USA.
EM nvolkow@nida.nih.gov
NR 0
TC 0
Z9 0
U1 0
U2 0
PU MASSACHUSETTS MEDICAL SOC
PI WALTHAM
PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA
SN 0028-4793
EI 1533-4406
J9 NEW ENGL J MED
JI N. Engl. J. Med.
PD JUL 7
PY 2016
VL 375
IS 1
BP 96
EP 96
PG 1
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ1ZP
UT WOS:000379000200027
PM 27406367
ER
PT J
AU Bronte, V
Brandau, S
Chen, SH
Colombo, MP
Frey, AB
Greten, TF
Mandruzzato, S
Murray, PJ
Ochoa, A
Ostrand-Rosenberg, S
Rodriguez, PC
Sica, A
Umansky, V
Vonderheide, RH
Gabrilovich, DI
AF Bronte, Vincenzo
Brandau, Sven
Chen, Shu-Hsia
Colombo, Mario P.
Frey, Alan B.
Greten, Tim F.
Mandruzzato, Susanna
Murray, Peter J.
Ochoa, Augusto
Ostrand-Rosenberg, Suzanne
Rodriguez, Paulo C.
Sica, Antonio
Umansky, Viktor
Vonderheide, Robert H.
Gabrilovich, Dmitry I.
TI Recommendations for myeloid-derived suppressor cell nomenclature and
characterization standards
SO NATURE COMMUNICATIONS
LA English
DT Review
ID TUMOR-BEARING MICE; T-CELLS; IMMUNE-RESPONSE; DENDRITIC CELLS; HUMAN
CANCERS; LUNG-CANCER; CROSS-TALK; IN-VIVO; MACROPHAGES; DIFFERENTIATION
AB Myeloid-derived suppressor cells (MDSCs) have emerged as major regulators of immune responses in cancer and other pathological conditions. In recent years, ample evidence supports key contributions of MDSC to tumour progression through both immune-mediated mechanisms and those not directly associated with immune suppression. MDSC are the subject of intensive research with >500 papers published in 2015 alone. However, the phenotypic, morphological and functional heterogeneity of these cells generates confusion in investigation and analysis of their roles in inflammatory responses. The purpose of this communication is to suggest characterization standards in the burgeoning field of MDSC research.
C1 [Bronte, Vincenzo] Univ Verona, Univ Hosp, Dept Med, I-37134 Verona, Italy.
[Brandau, Sven] Univ Hosp Essen, Dept Otorhinolaryngol, D-45122 Essen, Germany.
[Chen, Shu-Hsia] Icahn Sch Med Mt Sinai, Inst Immunol, Tisch Canc Inst, Dept Oncol Sci, New York, NY 10029 USA.
[Colombo, Mario P.] Fdn IRCCS Ist Nazl Tumori, Dept Expt Oncol & Mol Med, Mol Immunol Unit, I-20133 Milan, Italy.
[Frey, Alan B.] New York Univ, Sch Med, New York, NY 10029 USA.
[Greten, Tim F.] NCI, Thorac & GI Oncol Branch, GI Malignancy Sect, Bethesda, MD 20892 USA.
[Mandruzzato, Susanna] Univ Padua, Dept Surg Oncol & Gastroenterol, Sect Oncol & Immunol, I-35128 Padua, Italy.
[Mandruzzato, Susanna] IRCCS, Veneto Inst Oncol IOV, I-35128 Padua, Italy.
[Murray, Peter J.] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA.
[Murray, Peter J.] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA.
[Ochoa, Augusto] Louisiana State Univ, Stanley S Scott Canc Ctr, New Orleans, LA 70112 USA.
[Ostrand-Rosenberg, Suzanne] Univ Maryland Baltimore Cty, Baltimore, MD 21250 USA.
[Rodriguez, Paulo C.] Georgia Regents Univ, Ctr Canc, Augusta, GA 30912 USA.
[Sica, Antonio] Humanitas Clin & Res Ctr, Via Manzoni 56, I-20089 Milan, Italy.
[Sica, Antonio] Univ Piemonte Orientale Amedeo Avogadro, Dept Pharmaceut Sci, Via Bovio 6, I-20089 Novara, Italy.
[Umansky, Viktor] German Canc Res Ctr, Skin Canc Unit, D-69120 Heidelberg, Germany.
[Umansky, Viktor] Ruprecht Karl Univ Heidelberg, Univ Med Ctr Mannheim, Dept Dermatol Venereol & Allergol, D-69120 Mannheim, Germany.
[Vonderheide, Robert H.; Gabrilovich, Dmitry I.] Univ Penn, Abramson Canc Ctr, Sch Med, Philadelphia, PA 19104 USA.
[Gabrilovich, Dmitry I.] Wistar Inst Anat & Biol, Translat Tumor Immunol, Philadelphia, PA 19104 USA.
RP Bronte, V (reprint author), Univ Verona, Univ Hosp, Dept Med, I-37134 Verona, Italy.; Gabrilovich, DI (reprint author), Wistar Inst Anat & Biol, Translat Tumor Immunol, Philadelphia, PA 19104 USA.
EM vincenzo.bronte@univr.it; dgabrilovich@wistar.org
RI Bronte, Vincenzo/K-7902-2016
OI Bronte, Vincenzo/0000-0002-3741-5141
FU NIH [CA 141438, CA 084488, GM021248, CA 184185]; Italian Ministry of
Education, Universities and Research (FIRB cup) [B31J11000420001,
RBAP11H2R9_005]; Italian Ministry of Education, Universities and
Research (PRIN cup) [20103FMJEN_003]; Italian Association for Cancer
Research [AIRC 6599, 12182, 12886, 14103, 14194, 15585]; Italian
Ministry of Health (FINALIZZATA cup) [E35G1400019001]; Action BM1404
Mye-EUNITER - COST (European Cooperation in Science and Technology);
German Research Association (DFG) [2278/2-1]; American Lebanese Syrian
Associated Charities of St. Jude Children's Research Hospital [P30
CA21765]; American Lebanese Syrian Associated Charities of Cancer Center
Core grant [P30 CA21765]; DKFZ-MOST Cooperation in Cancer Research
[CA157]
FX This work was supported by NIH grants CA 141438 and CA 084488 to D.I.G.,
GM021248 to S.O.-R., CA 184185 to P.C.R., grants from the Italian
Ministry of Education, Universities and Research (FIRB cup:
B31J11000420001; FIRB cup: RBAP11H2R9_005; PRIN cup: 20103FMJEN_003) to
V.B. and A.S.; the Italian Association for Cancer Research (AIRC 6599,
12182, 12886, 14103, 14194, 14103 and 15585) and the Italian Ministry of
Health (FINALIZZATA cup: E35G1400019001) to V.B., M.P.C., A.S., S.M.;
Action BM1404 Mye-EUNITER supported by COST (European Cooperation in
Science and Technology) to S.B. and V.U.; the German Research
Association (DFG, grant 2278/2-1) to S.B., The American Lebanese Syrian
Associated Charities of St. Jude Children's Research Hospital and Cancer
Center Core grant P30 CA21765 to P.M. and the DKFZ-MOST Cooperation in
Cancer Research (CA157) to V.U.
NR 81
TC 33
Z9 34
U1 4
U2 4
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2041-1723
J9 NAT COMMUN
JI Nat. Commun.
PD JUL 6
PY 2016
VL 7
AR 12150
DI 10.1038/ncomms12150
PG 10
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA EH6ET
UT WOS:000391866300001
PM 27381735
ER
PT J
AU Alderson, TR
Kim, JH
Markley, JL
AF Alderson, Thomas Reid
Kim, Jin Hae
Markley, John Lute
TI Dynamical Structures of Hsp70 and Hsp70-Hsp40 Complexes
SO STRUCTURE
LA English
DT Review
ID NUCLEOTIDE-BINDING DOMAIN; ESCHERICHIA-COLI DNAJ; HEAT-SHOCK-PROTEIN;
X-RAY-SCATTERING; MOLECULAR CHAPERONE HSC70; SUBSTRATE-BINDING;
CONFORMATIONAL DYNAMICS; ALLOSTERIC REGULATION; INTERDOMAIN
COMMUNICATION; MITOCHONDRIAL HSP70
AB Protein misfolding and aggregation are pathological events that place a significant amount of stress on the maintenance of protein homeostasis (proteostasis). For prevention and repair of protein misfolding and aggregation, cells are equipped with robust mechanisms that mainly rely on molecular chaperones. Two classes of molecular chaperones, heat shock protein 70 kDa (Hsp70) and Hsp40, recognize and bind to mis-folded proteins, preventing their toxic biomolecular aggregation and enabling refolding or targeted degradation. Here, we review the current state of structural biology of Hsp70 and Hsp40-Hsp70 complexes and examine the link between their structures, dynamics, and functions. We highlight the power of nuclear magnetic resonance spectroscopy to untangle complex relationships behind molecular chaperones and their mechanism(s) of action.
C1 [Alderson, Thomas Reid] Univ Oxford, Dept Chem, South Parks Rd, Oxford OX1 3TA, England.
[Alderson, Thomas Reid] NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
[Kim, Jin Hae; Markley, John Lute] Univ Wisconsin, Dept Biochem, Natl Magnet Resonance Facil Madison, Madison, WI 53706 USA.
RP Alderson, TR (reprint author), Univ Oxford, Dept Chem, South Parks Rd, Oxford OX1 3TA, England.; Alderson, TR (reprint author), NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
EM reid.alderson@pmb.ox.ac.uk
FU NIH Oxford-Cambridge Scholars Program; US NIH [5U01GM094584]
FX The authors thank William Ford Freyberg, Justin L. P. Benesch, and Iva
Pritisanac for critically reading the manuscript and providing helpful
comments and suggestions. Supported in part by the NIH Oxford-Cambridge
Scholars Program and US NIH grant 5U01GM094584.
NR 117
TC 4
Z9 4
U1 17
U2 17
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0969-2126
EI 1878-4186
J9 STRUCTURE
JI Structure
PD JUL 6
PY 2016
VL 24
IS 7
BP 1014
EP 1030
DI 10.1016/j.str.2016.05.011
PG 17
WC Biochemistry & Molecular Biology; Biophysics; Cell Biology
SC Biochemistry & Molecular Biology; Biophysics; Cell Biology
GA DV9EU
UT WOS:000383243800002
PM 27345933
ER
PT J
AU DiMattia, MA
Watts, NR
Cheng, NQ
Huang, R
Heymann, JB
Grimes, JM
Wingfield, PT
Stuart, DI
Steven, AC
AF DiMattia, Michael A.
Watts, Norman R.
Cheng, Naiqian
Huang, Rick
Heymann, J. Bernard
Grimes, Jonathan M.
Wingfield, Paul T.
Stuart, David I.
Steven, Alasdair C.
TI The Structure of HIV-1 Rev Filaments Suggests a Bilateral Model for
Rev-RRE Assembly
SO STRUCTURE
LA English
DT Article
ID HUMAN-IMMUNODEFICIENCY-VIRUS; NUCLEAR EXPORT SIGNALS; RESPONSE ELEMENT
RRE; MESSENGER-RNA EXPORT; PROTEIN COMPLEXES; GENE-EXPRESSION;
BINDING-SITE; ALPHA-HELIX; TYPE-1; RECOGNITION
AB HIV-1 Rev protein mediates the nuclear export of viral RNA genomes. To do so, Rev oligomerizes cooperatively onto an RNA motif, the Rev response element (RRE), forming a complex that engages with the host nuclear export machinery. To better understand Rev oligomerization, we determined four crystal structures of Rev N-terminal domain dimers, which show that they can pivot about their dyad axis, giving crossing angles of 90 degrees to 140 degrees. In parallel, we performed cryoelectron microscopy of helical Rev filaments. Filaments vary from 11 to 15 nm in width, reflecting variations in dimer crossing angle. These structures contain additional density, indicating that C-terminal domains become partially ordered in the context of filaments. This conformational variability may be exploited in the assembly of RRE/Rev complexes. Our data also revealed a third interface between Revs, which offers an explanation for how the arrangement of Rev subunits adapts to the "A"-shaped architecture of the RRE in export-active complexes.
C1 [DiMattia, Michael A.; Cheng, Naiqian; Huang, Rick; Heymann, J. Bernard; Steven, Alasdair C.] NIAMSD, Lab Struct Biol Res, NIH, Bethesda, MD 20892 USA.
[Watts, Norman R.; Wingfield, Paul T.] NIAMSD, Prot Express Lab, NIH, Bethesda, MD 20892 USA.
[DiMattia, Michael A.; Grimes, Jonathan M.; Stuart, David I.] Univ Oxford, Div Struct Biol, Henry Wellcome Bldg Genom Med,Roosevelt Dr, Headington OX3 7BN, England.
[Grimes, Jonathan M.; Stuart, David I.] Diamond Light Source, Diamond House,Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
[DiMattia, Michael A.] Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, Struct Biol Program, 1275 York Ave, New York, NY 10065 USA.
[Huang, Rick] Howard Hughes Med Inst, Janelia Res Campus,19700 Helix Dr, Ashburn, VA 20147 USA.
RP Steven, AC (reprint author), NIAMSD, Lab Struct Biol Res, NIH, Bethesda, MD 20892 USA.
EM stevena@mail.nih.gov
FU National Institute for Arthritis and Musculoskeletal and Skin Diseases;
NIH; UK Medical Research Council [G100099]; SPINE2COMPLEXES
[LSHGST-2006-031220]; Wellcome Trust [075491/Z/09]
FX We thank Stephen J. Stahl, Ira Palmer, and Joshua Kaufman (NIH/NIAMS)
for support with preparation of reagents and Dennis Winkler (NIH/NIAMS)
for installation and calibration of SerialEM, and the staff at Diamond
Light Source (Didcot, UK) beamlines I02 and I24 and European Synchrotron
Radiation Facility (Grenoble, France) beamline ED23-EH2 for support in
data collection. This work was supported in part by the Intramural
Research Programs of the National Institute for Arthritis and
Musculoskeletal and Skin Diseases, the NIH Intramural AIDS Targeted
Antiviral Program, and the NIH-Oxford Scholars Program. D.I.S. is
supported by the UK Medical Research Council (G100099) and J.M.G. by
SPINE2COMPLEXES (LSHGST-2006-031220). The Wellcome Trust is acknowledged
for providing administrative support (Grant 075491/Z/09).
NR 56
TC 1
Z9 1
U1 1
U2 1
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0969-2126
EI 1878-4186
J9 STRUCTURE
JI Structure
PD JUL 6
PY 2016
VL 24
IS 7
BP 1068
EP 1080
DI 10.1016/j.str.2016.04.015
PG 13
WC Biochemistry & Molecular Biology; Biophysics; Cell Biology
SC Biochemistry & Molecular Biology; Biophysics; Cell Biology
GA DV9EU
UT WOS:000383243800006
PM 27265851
ER
PT J
AU Zacharchenko, T
Qian, XL
Goult, BT
Jethwa, D
Almeida, TB
Ballestrem, C
Critchley, DR
Lowy, DR
Barsukov, IL
AF Zacharchenko, Thomas
Qian, Xiaolan
Goult, Benjamin T.
Jethwa, Devina
Almeida, Teresa B.
Ballestrem, Christoph
Critchley, David R.
Lowy, Douglas R.
Barsukov, Igor L.
TI LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex
SO STRUCTURE
LA English
DT Article
ID FOCAL ADHESION KINASE; VINCULIN-BINDING-SITE; CELL-MIGRATION;
ACTIVATION; DOMAIN; ROD; RECRUITMENT; ACTIN; RIAM; FAK
AB Cell migration requires coordination between integrin-mediated cell adhesion to the extracellular matrix and force applied to adhesion sites. Talin plays a key role in coupling integrin receptors to the actomyosin contractile machinery, while deleted in liver cancer 1 (DLC1) is a Rho GAP that binds talin and regulates Rho, and therefore actomyosin contractility. We show that the LD motif of DLC1 forms a helix that binds to the four-helix bundle of the talin R8 domain in a canonical triple-helix arrangement. We demonstrate that the same R8 surface interacts with the paxillin LD1 and LD2 motifs. We identify key charged residues that stabilize the R8 interactions with LD motifs and demonstrate their importance in vitro and in cells. Our results suggest a network of competitive interactions in adhesion complexes that involve LD motifs, and identify mutations that can be used to analyze the biological roles of specific protein-protein interactions in cell migration.
C1 [Zacharchenko, Thomas; Almeida, Teresa B.; Barsukov, Igor L.] Univ Liverpool, Inst Integrat Biol, BioSci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England.
[Qian, Xiaolan; Lowy, Douglas R.] NCI, Cellular Oncol Lab, NIH, Bethesda, MD 20892 USA.
[Goult, Benjamin T.] Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England.
[Jethwa, Devina; Ballestrem, Christoph] Univ Manchester, Wellcome Trust Ctr Cell Matrix Res, Oxford Rd, Manchester M13 9PT, Lancs, England.
[Critchley, David R.] Univ Leicester, Dept Biochem, Lancaster Rd, Leicester LE1 9HN, Leics, England.
RP Barsukov, IL (reprint author), Univ Liverpool, Inst Integrat Biol, BioSci Bldg,Crown St, Liverpool L69 7ZB, Merseyside, England.
EM igb2@liv.ac.uk
OI Goult, Ben/0000-0002-3438-2807
FU Biotechnology and Biological Sciences Research Council (BBSRC) DTP
fellowship; BBSRC [BB/N007336/1]; Wellcome Trust [088785/Z/09/Z]; BBSRC
DTP studentship
FX T.Z. was supported by the Biotechnology and Biological Sciences Research
Council (BBSRC) DTP fellowship. B.T.G is funded by BBSRC (BB/N007336/1).
The C.B. laboratory is part of the Wellcome Trust Centre for Cell-Matrix
Research, University of Manchester, which is supported by core funding
from the Wellcome Trust (grant number 088785/Z/09/Z). D.J. is supported
by a BBSRC DTP studentship. We thank the staff of the Bioimaging
facility at the University of Manchester for help with imaging. The
X-ray data were collected at the Diamond Light Source under Liverpool
BAG allocation.
NR 34
TC 0
Z9 0
U1 5
U2 6
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0969-2126
EI 1878-4186
J9 STRUCTURE
JI Structure
PD JUL 6
PY 2016
VL 24
IS 7
BP 1130
EP 1141
DI 10.1016/j.str.2016.04.016
PG 12
WC Biochemistry & Molecular Biology; Biophysics; Cell Biology
SC Biochemistry & Molecular Biology; Biophysics; Cell Biology
GA DV9EU
UT WOS:000383243800011
PM 27265849
ER
PT J
AU Takahashi, YK
Langdon, AJ
Niv, Y
Schoenbaum, G
AF Takahashi, Yuji K.
Langdon, Angela J.
Niv, Yael
Schoenbaum, Geoffrey
TI Temporal Specificity of Reward Prediction Errors Signaled by Putative
Dopamine Neurons in Rat VTA Depends on Ventral Striatum
SO NEURON
LA English
DT Article
ID TEGMENTAL AREA; NUCLEUS-ACCUMBENS; ORBITOFRONTAL CORTEX; DIFFERENCE
MODELS; DORSAL STRIATUM; BASAL GANGLIA; SIZED REWARDS; TIME;
REPRESENTATION; HIPPOCAMPUS
AB Dopamine neurons signal reward prediction errors. This requires accurate reward predictions. It has been suggested that the ventral striatum provides these predictions. Here we tested this hypothesis by recording from putative dopamine neurons in the VTA of rats performing a task in which prediction errors were induced by shifting reward timing or number. In controls, the neurons exhibited error signals in response to both manipulations. However, dopamine neurons in rats with ipsilateral ventral striatal lesions exhibited errors only to changes in number and failed to respond to changes in timing of reward. These results, supported by computational modeling, indicate that predictions about the temporal specificity and the number of expected reward are dissociable and that dopaminergic prediction-error signals rely on the ventral striatum for the former but not the latter.
C1 [Takahashi, Yuji K.; Schoenbaum, Geoffrey] NIDA, Intramural Res Program, Baltimore, MD 21224 USA.
[Langdon, Angela J.; Niv, Yael] Princeton Univ, Dept Psychol, Princeton, NJ 08544 USA.
[Langdon, Angela J.; Niv, Yael] Princeton Univ, Inst Neurosci, Princeton, NJ 08544 USA.
[Schoenbaum, Geoffrey] Univ Maryland, Sch Med, Dept Anat & Neurobiol, Baltimore, MD 21201 USA.
[Schoenbaum, Geoffrey] Univ Maryland, Sch Med, Dept Psychiat, Baltimore, MD 21201 USA.
[Schoenbaum, Geoffrey] Johns Hopkins Univ, Solomon H Snyder Dept Neurosci, Baltimore, MD 21287 USA.
RP Takahashi, YK; Schoenbaum, G (reprint author), NIDA, Intramural Res Program, Baltimore, MD 21224 USA.; Schoenbaum, G (reprint author), Univ Maryland, Sch Med, Dept Anat & Neurobiol, Baltimore, MD 21201 USA.; Schoenbaum, G (reprint author), Univ Maryland, Sch Med, Dept Psychiat, Baltimore, MD 21201 USA.; Schoenbaum, G (reprint author), Johns Hopkins Univ, Solomon H Snyder Dept Neurosci, Baltimore, MD 21287 USA.
EM yuji.takahashi@nih.gov; geoffrey.schoenbaum@nih.gov
FU NIDA; Human Frontier Science Program Organization; NIMH [R01MH098861]
FX This work was supported by funding from NIDA (to Y.K.T. and G.S.), the
Human Frontier Science Program Organization (to A.J.L.), and NIMH grant
R01MH098861 (to Y.N.). The opinions expressed in this article are the
authors' own and do not reflect the view of the NIH, the Department of
Health and Human Services, or the United States government. The authors
would like to acknowledge Nathaniel Daw for helpful suggestions
regarding the semi-Markov model of the task.
NR 56
TC 8
Z9 8
U1 4
U2 5
PU CELL PRESS
PI CAMBRIDGE
PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
SN 0896-6273
EI 1097-4199
J9 NEURON
JI Neuron
PD JUL 6
PY 2016
VL 91
IS 1
BP 182
EP 193
DI 10.1016/j.neuron.2016.05.015
PG 12
WC Neurosciences
SC Neurosciences & Neurology
GA DU7KY
UT WOS:000382394300021
PM 27292535
ER
PT J
AU Goossens, ME
Isa, F
Brinkman, M
Mak, D
Reulen, R
Wesselius, A
Benhamou, S
Bosetti, C
Bueno-de-Mesquita, B
Carta, A
Allam, MF
Golka, K
Grant, EJ
Jiang, XJ
Johnson, KC
Karagas, MR
Kellen, E
La Vecchia, C
Lu, CM
Marshall, J
Moysich, K
Pohlabeln, H
Porru, S
Steineck, G
Stern, MC
Tang, L
Taylor, JA
van den Brandt, P
Villeneuve, PJ
Wakai, K
Weiderpass, E
White, E
Wolk, A
Zhang, ZF
Buntinx, F
Zeegers, MP
AF Goossens, Maria E.
Isa, Fatima
Brinkman, Maree
Mak, David
Reulen, Raoul
Wesselius, Anke
Benhamou, Simone
Bosetti, Cristina
Bueno-de-Mesquita, Bas
Carta, Angela
Allam, Md Farouk
Golka, Klaus
Grant, Eric J.
Jiang, Xuejuan
Johnson, Kenneth C.
Karagas, Margaret R.
Kellen, Eliane
La Vecchia, Carlo
Lu, Chih-Ming
Marshall, James
Moysich, Kirsten
Pohlabeln, Hermann
Porru, Stefano
Steineck, Gunnar
Stern, Marianne C.
Tang, Li
Taylor, Jack A.
van den Brandt, Piet
Villeneuve, Paul J.
Wakai, Kenji
Weiderpass, Elisabete
White, Emily
Wolk, Alicja
Zhang, Zuo-Feng
Buntinx, Frank
Zeegers, Maurice P.
TI International pooled study on diet and bladder cancer: the bladder
cancer, epidemiology and nutritional determinants (BLEND) study: design
and baseline characteristics
SO ARCHIVES OF PUBLIC HEALTH
LA English
DT Article
DE Bladder cancer; Diet; Risk; Pooled analysis
ID RISK-FACTORS; FLUID INTAKE; CONSUMPTION; RATIONALE; SMOKING; COHORT;
POLYMORPHISMS; VITAMINS; SYSTEM; FRUIT
AB Background: In 2012, more than 400,000 urinary bladder cancer cases occurred worldwide, making it the 7th most common type of cancer. Although many previous studies focused on the relationship between diet and bladder cancer, the evidence related to specific food items or nutrients that could be involved in the development of bladder cancer remains inconclusive. Dietary components can either be, or be activated into, potential carcinogens through metabolism, or act to prevent carcinogen damage.
Methods/design: The BLadder cancer, Epidemiology and Nutritional Determinants (BLEND) study was set up with the purpose of collecting individual patient data from observational studies on diet and bladder cancer. In total, data from 11,261 bladder cancer cases and 675,532 non-cases from 18 case-control and 6 cohort studies from all over the world were included with the aim to investigate the association between individual food items, nutrients and dietary patterns and risk of developing bladder cancer.
Discussion: The substantial number of cases included in this study will enable us to provide evidence with large statistical power, for dietary recommendations on the prevention of bladder cancer.
C1 [Goossens, Maria E.; Buntinx, Frank] Katholieke Univ Leuven, ACHG KU Leuven, Dept Gen Practice, Kapucijnenvoer 33,Blok J,Bus 7001, B-3000 Leuven, Belgium.
[Isa, Fatima; Mak, David; Reulen, Raoul] Univ Birmingham, Dept Publ Hlth Epidemiol & Biostat, Birmingham, W Midlands, England.
[Brinkman, Maree] Canc Council Victoria, Melbourne, Vic, Australia.
[Wesselius, Anke; Zeegers, Maurice P.] Maastricht Univ, NUTRIM Sch Nutr & Translat Res Metab, Maastricht, Netherlands.
[Benhamou, Simone] Fondat Jean Dausset CEPH, Variabilite Genet & Malad Humaines, INSERM U946, Paris, France.
[Bosetti, Cristina] Ist Ric Farmacol Mario Negri, Lab Gen Epidemiol, Milan, Italy.
[Bueno-de-Mesquita, Bas] Natl Inst Publ Hlth & Environm RIVM, DCD, Bilthoven, Netherlands.
[Bueno-de-Mesquita, Bas] Univ Med Ctr, Gastroenterol & Hepatol, Utrecht, Netherlands.
[Bueno-de-Mesquita, Bas] Univ London Imperial Coll Sci Technol & Med, Sch Publ Hlth, Epidemiol & Biostat, London, England.
[Bueno-de-Mesquita, Bas] Univ Malaya, Social & Prevent Med, Fac Med, Kuala Lumpur, Malaysia.
[Carta, Angela; Porru, Stefano] Univ Brescia, Sect Publ Hlth & Human Sci, Dept Med & Surg Specialties Radiol Sci & Publ Hlt, Brescia, Italy.
[Allam, Md Farouk] Univ Cordoba, Dept Prevent Med & Publ Hlth, Fac Med, Cordoba, Spain.
[Golka, Klaus] TU Dortmund, Leibniz Res Ctr Working Environm & Human Factors, Dortmund, Germany.
[Grant, Eric J.] Dept Epidemiol Radiat Effects Res Fdn, Hiroshima, Japan.
[Jiang, Xuejuan; Stern, Marianne C.] Univ So Calif, Dept Prevent Med, Los Angeles, CA 90089 USA.
[Johnson, Kenneth C.] Univ Ottawa, Dept Epidemiol & Community Med, Ottawa, ON, Canada.
[Karagas, Margaret R.] Geisel Sch Med Dartmouth, Dept Epidemiol, Hanover, NH USA.
[Kellen, Eliane] Leuven Univ Ctr Canc Prevent LUCK, Leuven, Belgium.
[La Vecchia, Carlo] Univ Milan, Dept Clin Med & Community Hlth, Milan, Italy.
[Lu, Chih-Ming] Buddhist Dalin Tzu Chi Gen Hosp, Dept Urol, Dalin Township 62247, Chiayi County, Taiwan.
[Marshall, James; Moysich, Kirsten; Tang, Li] Roswell Pk Canc Inst, Dept Canc Prevent & Control, Buffalo, NY 14263 USA.
[Pohlabeln, Hermann] Leibniz Inst Prevent Res & Epidemiol BIPS, Bremen, Germany.
[Steineck, Gunnar] Karolinska Hosp, Div Clin Canc Epidemiol, Dept Oncol & Pathol, Stockholm, Sweden.
[Taylor, Jack A.] Natl Inst Environm Hlth Sci, Epidemiol Branch, NIH, Res Triangle Pk, NC USA.
[Taylor, Jack A.] Natl Inst Environm Hlth Sci, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC USA.
[van den Brandt, Piet] Maastricht Univ, Dept Epidemiol, Sch Oncol & Dev Biol, Med Ctr, Maastricht, Netherlands.
[van den Brandt, Piet] Maastricht Univ, Med Ctr, Sch Publ Hlth & Primary Care, Maastricht, Netherlands.
[Villeneuve, Paul J.] Populat Studies Div Hlth Canada, Ottawa, ON, Canada.
[Wakai, Kenji] Nagoya Univ, Dept Prevent Med, Grad Sch Med, Nagoya, Aichi, Japan.
[Weiderpass, Elisabete] Karolinska Inst, Dept Med Epidemiol & Biostat, Med Epidemiol, Stockholm, Sweden.
[Weiderpass, Elisabete] Canc Registry Norway, Inst Populat Based Canc Res, Res, Oslo, Norway.
[Weiderpass, Elisabete] Folkhalsan Res Ctr, Genet Epidemiol Grp, Helsinki, Finland.
[Weiderpass, Elisabete] Univ Tromso, Arctic Univ Norway, Dept Community Med, Tromso, Norway.
[White, Emily] Fred Hutchinson Canc Res Ctr, 1124 Columbia St, Seattle, WA 98104 USA.
[Wolk, Alicja] Karolinska Inst, Inst Environm Med, Div Nutr Epidemiol, Stockholm, Sweden.
[Zhang, Zuo-Feng] Univ Calif Los Angeles, Fielding Sch Publ Hlth, UCLA Ctr Environm Genom, Dept Epidemiol, Los Angeles, CA USA.
[Buntinx, Frank; Zeegers, Maurice P.] Maastricht Univ, CAPHRI Sch Publ Hlth & Primary Care, Maastricht, Netherlands.
[Zeegers, Maurice P.] Univ Birmingham, Sch Canc Sci, Birmingham, W Midlands, England.
RP Goossens, ME (reprint author), Katholieke Univ Leuven, ACHG KU Leuven, Dept Gen Practice, Kapucijnenvoer 33,Blok J,Bus 7001, B-3000 Leuven, Belgium.
EM mieke.goossens@med.kuleuven.be
RI Benhamou, Simone/K-6554-2015; Weiderpass, Elisabete/M-4029-2016;
OI Weiderpass, Elisabete/0000-0003-2237-0128; La Vecchia,
Carlo/0000-0003-1441-897X; taylor, jack/0000-0001-5303-6398
FU NCI NIH HHS [P30 CA016056, R01 CA057494, R01 CA074846, T32 CA009142, U01
CA096116]; NIEHS NIH HHS [P42 ES007373]
NR 40
TC 0
Z9 0
U1 1
U2 2
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 0778-7367
EI 2049-3258
J9 ARCH PUBLIC HEALTH
JI Arch. Public Health
PD JUL 6
PY 2016
VL 74
AR 30
DI 10.1186/s13690-016-0140-1
PG 10
WC Public, Environmental & Occupational Health
SC Public, Environmental & Occupational Health
GA DS0ZI
UT WOS:000380325100001
PM 27386115
ER
PT J
AU Thomas, A
Pommier, Y
AF Thomas, Anish
Pommier, Yves
TI Small cell lung cancer: Time to revisit DNA-damaging chemotherapy
SO SCIENCE TRANSLATIONAL MEDICINE
LA English
DT Editorial Material
ID ATR INHIBITORS; STRESS
AB Rational use of DNA-damaging chemotherapy, with new combinations to heighten DNA replication stress, could improve outcomes in small cell lung cancer.
C1 [Thomas, Anish] NCI, Thorac & Gastrointestinal Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA.
[Pommier, Yves] NCI, Dev Therapeut Branch, Ctr Canc Res, Bethesda, MD 20892 USA.
[Pommier, Yves] NCI, Mol Pharmacol Lab, Ctr Canc Res, Bethesda, MD 20892 USA.
RP Thomas, A (reprint author), NCI, Thorac & Gastrointestinal Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA.; Pommier, Y (reprint author), NCI, Dev Therapeut Branch, Ctr Canc Res, Bethesda, MD 20892 USA.; Pommier, Y (reprint author), NCI, Mol Pharmacol Lab, Ctr Canc Res, Bethesda, MD 20892 USA.
EM anish.thomas@mail.nih.gov; pommier@nih.gov
NR 10
TC 1
Z9 1
U1 0
U2 1
PU AMER ASSOC ADVANCEMENT SCIENCE
PI WASHINGTON
PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA
SN 1946-6234
EI 1946-6242
J9 SCI TRANSL MED
JI Sci. Transl. Med.
PD JUL 6
PY 2016
VL 8
IS 346
AR 346fs12
DI 10.1126/scitranslmed.aaf6282
PG 3
WC Cell Biology; Medicine, Research & Experimental
SC Cell Biology; Research & Experimental Medicine
GA DR6RP
UT WOS:000380029300001
PM 27384345
ER
PT J
AU Beachler, DC
Yanik, EL
Martin, BI
Pfeiffer, RM
Mirza, SK
Deyo, RA
Engels, EA
AF Beachler, Daniel C.
Yanik, Elizabeth L.
Martin, Brook I.
Pfeiffer, Ruth M.
Mirza, Sohail K.
Deyo, Richard A.
Engels, Eric A.
TI Bone Morphogenetic Protein Use and Cancer Risk Among Patients Undergoing
Lumbar Arthrodesis
SO JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
LA English
DT Article
ID SPINAL ARTHRODESIS; FUSION; SURVEILLANCE; VALIDATION; SURGERY; DESIGN;
COHORT; CLAIMS
AB Background: Recombinant bone morphogenetic proteins (BMPs) are growth factors utilized in lumbar arthrodeses. Limited data from randomized trials suggest that BMP may increase cancer risk. We sought to evaluate cancer risk and mortality following the use of BMP in lumbar arthrodesis.
Methods: Within the linked Surveillance, Epidemiology, and End Results (SEER) Program-Medicare cohort, we conducted a case-cohort study of 7,278 individuals who were >= 65 years of age and had undergone a lumbar arthrodesis from 2004 to 2011. Of these patients, 3,627 were individuals in a 5% random subcohort of Medicare enrollees in SEER areas including 191 who developed cancer, and there were 3,651 individuals outside the subcohort who developed cancer. Weighted Cox proportional-hazards regression was used to estimate hazard ratios (HRs) and 95% confidence intervals (95% CIs) for cancer on the basis of exposure to BMP.
Results: In the SEER-Medicare subcohort, 30.7% of individuals who underwent a lumbar arthrodesis received BMP. BMP was not associated with overall cancer risk in univariate analyses (HR, 0.92 [95% CI, 0.82 to 1.02]) or after adjustment for demographic characteristics, comorbidities, hospital size, history of cancer, and calendar year (adjusted HR, 0.94 [95% CI, 0.84 to 1.05]). Individual cancer types were also not significantly elevated (p > 0.05 for all) in BMP users compared with nonusers. In addition, BMP use was not associated with a new cancer in people who had cancer prior to undergoing lumbar arthrodesis (adjusted HR, 1.04 [95% CI, 0.71 to 1.52]) or withmortality after a cancer diagnosis (adjusted HR, 1.05 [95% CI, 0.93 to 1.19]).
Conclusions: In a large population of elderly U.S. adults undergoing lumbar arthrodesis, BMP use was not associated with cancer risk or mortality.
C1 [Beachler, Daniel C.; Yanik, Elizabeth L.; Martin, Brook I.; Pfeiffer, Ruth M.; Mirza, Sohail K.; Deyo, Richard A.; Engels, Eric A.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.
[Martin, Brook I.; Mirza, Sohail K.] Dartmouth Geisel Sch Med, Dartmouth Inst Hlth Policy & Clin Practice, Hanover, NH USA.
[Mirza, Sohail K.] Dartmouth Geisel Sch Med, Dept Orthopaed Surg, Hanover, NH USA.
[Deyo, Richard A.] Oregon Hlth & Sci Univ, Dept Family Med, Portland, OR 97201 USA.
[Deyo, Richard A.] Oregon Hlth & Sci Univ, Dept Med, Portland, OR 97201 USA.
[Deyo, Richard A.] Oregon Hlth & Sci Univ, Dept Publ Hlth & Preventat Med, Portland, OR 97201 USA.
[Deyo, Richard A.] Oregon Hlth & Sci Univ, Oregon Inst Occupat Hlth Sci, Portland, OR 97201 USA.
RP Beachler, DC (reprint author), NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.
EM Daniel.beachler@nih.gov
FU National Institute of Arthritis and Musculoskeletal and Skin Diseases;
National Institutes of Health and the Agency for Healthcare Research and
Quality
FX One author of this study (B.I.M.) received, through his institution, a
grant from the National Institute of Arthritis and Musculoskeletal and
Skin Diseases; funds were used to pay for salary support. One author of
this study (S.K.M.) received grants from the National Institutes of
Health and the Agency for Healthcare Research and Quality; funds were
used to pay for salary support. One author of this study (R.A.D.)
received, through his institution, a gift from Kaiser Permanente; funds
were used to pay for an endowed chair that supports part of his salary.
NR 28
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U1 0
U2 0
PU LIPPINCOTT WILLIAMS & WILKINS
PI PHILADELPHIA
PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA
SN 0021-9355
EI 1535-1386
J9 J BONE JOINT SURG AM
JI J. Bone Joint Surg.-Am. Vol.
PD JUL 6
PY 2016
VL 98
IS 13
BP 1064
EP 1072
DI 10.2106/JBJS.15.01106
PG 9
WC Orthopedics; Surgery
SC Orthopedics; Surgery
GA DR5ID
UT WOS:000379935800005
PM 27385679
ER
PT J
AU Markunas, CA
Wilcox, AJ
Xu, ZL
Joubert, BR
Harlid, S
Panduri, V
Haberg, SE
Nystad, W
London, SJ
Sandler, DP
Lie, RT
Wade, PA
Taylor, JA
AF Markunas, Christina A.
Wilcox, Allen J.
Xu, Zongli
Joubert, Bonnie R.
Harlid, Sophia
Panduri, Vijayalakshmi
Haberg, Siri E.
Nystad, Wenche
London, Stephanie J.
Sandler, Dale P.
Lie, Rolv T.
Wade, Paul A.
Taylor, Jack A.
TI Maternal Age at Delivery Is Associated with an Epigenetic Signature in
Both Newborns and Adults
SO PLOS ONE
LA English
DT Article
ID DNA METHYLATION CHANGES; DEVELOPMENTAL ORIGINS; NORWEGIAN MOTHER; CHILD
COHORT; PREGNANCY; EXPRESSION; SMOKING; RISK; PERSPECTIVE; POPULATION
AB Offspring of older mothers are at increased risk of adverse birth outcomes, childhood cancers, type 1 diabetes, and neurodevelopmental disorders. The underlying biologic mechanisms for most of these associations remain obscure. One possibility is that maternal aging may produce lasting changes in the epigenetic features of a child's DNA. To test this, we explored the association of mothers' age at pregnancy with methylation in her offspring, using blood samples from 890 Norwegian newborns and measuring DNA methylation at more than 450,000 CpG sites across the genome. We examined replication of a maternal-age finding in an independent group of 1062 Norwegian newborns, and then in 200 US middle-aged women. Older maternal age was significantly associated with reduced methylation at four adjacent CpGs near the 2nd exon of KLHL35 in newborns (p-values ranging from 3x10(-6) to 8x10(-7)). These associations were replicated in the independent set of newborns, and replicated again in women 40 to 60 years after their birth. This study provides the first example of parental age permanently affecting the epigenetic profile of offspring. While the specific functions of the affected gene are unknown, this finding opens the possibility that a mother's age at pregnancy could affect her child's health through epigenetic mechanisms.
C1 [Markunas, Christina A.; Wilcox, Allen J.; Xu, Zongli; Joubert, Bonnie R.; London, Stephanie J.; Sandler, Dale P.; Taylor, Jack A.] NIEHS, Epidemiol Branch, NIH, Res Triangle Pk, NC 27709 USA.
[Harlid, Sophia; Panduri, Vijayalakshmi; Wade, Paul A.; Taylor, Jack A.] NIEHS, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC 27709 USA.
[Haberg, Siri E.; Nystad, Wenche; Lie, Rolv T.] Norwegian Inst Publ Hlth, Oslo, Norway.
[Lie, Rolv T.] Univ Bergen, Dept Global Publ Hlth & Primary Care, Bergen, Norway.
[Markunas, Christina A.] RTI Int, Res Triangle Pk, NC USA.
RP Taylor, JA (reprint author), NIEHS, Epidemiol Branch, NIH, Res Triangle Pk, NC 27709 USA.; Taylor, JA (reprint author), NIEHS, Epigenet & Stem Cell Biol Lab, NIH, Res Triangle Pk, NC 27709 USA.
EM taylor@niehs.nih.gov
OI Harlid, Sophia/0000-0001-8540-6891; xu, zongli/0000-0002-9034-8902;
Wilcox, Allen/0000-0002-3376-1311; taylor, jack/0000-0001-5303-6398;
Sandler, Dale/0000-0002-6776-0018; London, Stephanie/0000-0003-4911-5290
FU Intramural Research Program of the NIH, National Institute of
Environmental Health Science, Sister Study [Z01 ES044005]; Intramural
Research Program of the NIH, National Institute of Environmental Health
Science, MoBa [Z01-ES-49019]; Intramural Research Program of the NIH,
National Institute of Environmental Health Science, Norway Facial Clefts
Study; Norwegian Ministry of Health; Ministry of Education and Research;
NIH/NIEHS [N01-ES-75558]; NIH/NINDS [1 UO1 NS 047537-01]; Norwegian
Research Council/FUGE [151918/S10]
FX This research was supported in part by the Intramural Research Program
of the NIH, National Institute of Environmental Health Sciences
(http://www.niehs.nih.gov/, Z01 ES044005, Sister Study (DPS);
Z01-ES-49019, MoBa (SJL); Norway Facial Clefts Study (AJW)). The
Norwegian Mother and Child Cohort Study is supported by the Norwegian
Ministry of Health and the Ministry of Education and Research
(https://www.regjeringen.no/en/dep/kd/id586/), NIH/NIEHS
(http://www.niehs.nih.gov/, contract no N01-ES-75558), NIH/NINDS
(http://www.ninds.nih.gov/, grant no. 1 UO1 NS 047537-01), and the
Norwegian Research Council/FUGE
(http://www.forskningsradet.no/prognett-fuge/Home_page/1226993493114,
grant no. 151918/S10 (WN)). The funders had no role in study design,
data collection and analysis, decision to publish, or preparation of the
manuscript.
NR 34
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U1 0
U2 0
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 6
PY 2016
VL 11
IS 7
AR e0156361
DI 10.1371/journal.pone.0156361
PG 11
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR3NH
UT WOS:000379809400003
PM 27383059
ER
PT J
AU Fernando, RI
Hamilton, DH
Dominguez, C
David, JM
McCampbell, KK
Palena, C
AF Fernando, Romaine I.
Hamilton, Duane H.
Dominguez, Charli
David, Justin M.
McCampbell, Kristen K.
Palena, Claudia
TI IL-8 signaling is involved in resistance of lung carcinoma cells to
erlotinib
SO ONCOTARGET
LA English
DT Article
DE erlotinib; tumor resistance; IL-8; epithelial-mesenchymal transition
ID EPITHELIAL-MESENCHYMAL TRANSITION; TRANSCRIPTION FACTOR BRACHYURY; EGFR
KINASE DOMAIN; ACQUIRED-RESISTANCE; TARGETED THERAPY;
COLORECTAL-CARCINOMA; MONOCLONAL-ANTIBODY; MET AMPLIFICATION; CANCER;
GROWTH
AB A signaling pathway that is frequently deregulated in human carcinomas and has been explored as a therapeutic target involves the activation of the epidermal growth factor receptor (EGFR). Inhibition of EGFR via the small molecule inhibitors erlotinib and gefitinib commonly results in tumor resistance, even in patients with EGFR-mutant tumors that initially show substantial clinical responses. This study was designed to broaden our understanding of the molecular mechanisms of acquired resistance to erlotinib in lung cancer cells bearing wild type or mutated EGFR. We report here that generation of erlotinib-resistant lung cancer cells in vitro resulted in a phenotypic alteration reminiscent of an epithelial-mesenchymal transition (EMT) concomitant with a robust upregulation of the IL-8/IL-8R axis. Our results also demonstrate that upregulation of p38 MAPK signaling is responsible for the enhanced IL-8 secretion in the erlotinib-resistant tumor cells. Blockade of IL-8 signaling effectively reduced mesenchymal features of the resistant cells and also markedly enhanced their susceptibility to erlotinib. These results provide a rationale for the development of new therapeutic approaches involving blockade of IL-8 signaling for the management of acquired resistance to EGFR inhibition in patients with lung cancer.
C1 [Fernando, Romaine I.; Hamilton, Duane H.; Dominguez, Charli; David, Justin M.; McCampbell, Kristen K.; Palena, Claudia] NCI, Lab Tumor Immunol & Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
RP Palena, C (reprint author), NCI, Lab Tumor Immunol & Biol, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
EM palenac@mail.nih.gov
FU NIH Intramural Research Program, National Cancer Institute, Center for
Cancer Research
FX This research was supported by funds of the NIH Intramural Research
Program, National Cancer Institute, Center for Cancer Research.
NR 50
TC 3
Z9 3
U1 2
U2 2
PU IMPACT JOURNALS LLC
PI ALBANY
PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA
SN 1949-2553
J9 ONCOTARGET
JI Oncotarget
PD JUL 5
PY 2016
VL 7
IS 27
BP 42031
EP 42044
DI 10.18632/oncotarget.9662
PG 14
WC Oncology; Cell Biology
SC Oncology; Cell Biology
GA DS7DI
UT WOS:000380942600084
PM 27248176
ER
PT J
AU Zhang, Q
Doucet, M
Tomlinson, RE
Han, XB
Quarles, LD
Collins, MT
Clemens, TL
AF Zhang, Qian
Doucet, Michele
Tomlinson, Ryan E.
Han, Xiaobin
Quarles, L. Darryl
Collins, Michael T.
Clemens, Thomas L.
TI The hypoxia-inducible factor-1 alpha activates ectopic production of
fibroblast growth factor 23 in tumor-induced osteomalacia
SO Bone Research
LA English
DT Article
ID OF-THE-LITERATURE; RICKETS; FGF-23; FGF23
AB Tumor-induced osteomalacia (TIO) is a rare paraneoplastic syndrome in which ectopic production of fibroblast growth factor 23 (FGF23) by non-malignant mesenchymal tumors causes phosphate wasting and bone fractures. Recent studies have implicated the hypoxia-inducible factor-1 alpha (HIF-1 alpha) in other phosphate wasting disorders caused by elevated FGF23, including X-linked hypophosphatemic rickets and autosomal dominant hypophosphatemia. Here we provide evidence that HIF-1 alpha mediates aberrant FGF23 in TIO by transcriptionally activating its promoter. Immunohistochemical studies in phosphaturic mesenchymal tumors resected from patients with documented TIO showed that HIF-1 alpha and FGF23 were co-localized in spindle-shaped cells adjacent to blood vessels. Cultured tumor tissue produced high levels of intact FGF23 and demonstrated increased expression of HIF-1 alpha protein. Transfection of MC3T3-E1 and Saos-2 cells with a HIF-1 alpha expression construct induced the activity of a FGF23 reporter construct. Prior treatment of tumor organ cultures with HIF-1 alpha inhibitors decreased HIF-1 alpha and FGF23 protein accumulation and inhibited HIF-1 alpha-induced luciferase reporter activity in transfected cells. Chromatin immunoprecipitation assays confirmed binding to a HIF-1 alpha consensus sequence within the proximal FGF23 promoter, which was eliminated by treatment with a HIF-1 alpha inhibitor. These results show for the first time that HIF-1 alpha is a direct transcriptional activator of FGF23 and suggest that upregulation of HIF-1 alpha activity in TIO contributes to the aberrant FGF23 production in these patients.
C1 [Zhang, Qian; Doucet, Michele; Tomlinson, Ryan E.; Clemens, Thomas L.] Johns Hopkins Univ, Dept Orthopaed Surg, Baltimore, MD USA.
[Han, Xiaobin; Quarles, L. Darryl] Univ Tennessee, Hlth Sci Ctr, Dept Med, Memphis, TN USA.
[Collins, Michael T.] NIH, Skeletal Clin Studies Unit, Craniofacial & Skeletal Dis Branch, Bethesda, MD USA.
[Clemens, Thomas L.] Baltimore Vet Adm Med Ctr, Baltimore, MD USA.
RP Clemens, TL (reprint author), Johns Hopkins Univ, Dept Orthopaed Surg, Baltimore, MD USA.; Clemens, TL (reprint author), Baltimore Vet Adm Med Ctr, Baltimore, MD USA.
EM tclemen5@jhmi.edu
FU NIH [AR049510, AR045955]; Department of Veterans Affairs
FX This work was supported by NIH grants AR049510 (TLC) and AR045955 (LDQ).
TLC is the recipient of a Senior Career Scientist Award from the
Department of Veterans Affairs. We thank Dr Edward McCarthy for advice
on the histopathology of the tumor specimens.
NR 18
TC 1
Z9 1
U1 2
U2 2
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2095-4700
EI 2095-6231
J9 BONE RES
JI Bone Res.
PD JUL 5
PY 2016
VL 4
AR 16011
DI 10.1038/boneres.2016.11
PG 6
WC Cell & Tissue Engineering
SC Cell Biology
GA DS5CM
UT WOS:000380798200001
PM 27468359
ER
PT J
AU Ambrosis, N
Boyd, CD
O'Toole, GA
Fernandez, J
Sisti, F
AF Ambrosis, Nicolas
Boyd, Chelsea D.
O'Toole, George A.
Fernandez, Julieta
Sisti, Federico
TI Homologs of the LapD-LapG c-di-GMP Effector System Control Biofilm
Formation by Bordetella bronchiseptica
SO PLOS ONE
LA English
DT Article
ID PSEUDOMONAS-FLUORESCENS PF0-1; ADENYLATE-CYCLASE TOXIN;
RESPIRATORY-TRACT; MECHANISM; INFECTION; VIRULENCE; PERTUSSIS; MOTILITY;
PROTEASE
AB Biofilm formation is important for infection by many pathogens. Bordetella bronchiseptica causes respiratory tract infections in mammals and forms biofilm structures in nasal epithelium of infected mice. We previously demonstrated that cyclic di-GMP is involved in biofilm formation in B. bronchiseptica. In the present work, based on their previously reported function in Pseudomonas fluorescens, we identified three genes in the B. bronchiseptica genome likely involved in c-di-GMP-dependent biofilm formation: brtA, lapD and lapG. Genetic analysis confirmed a role for BrtA, LapD and LapG in biofilm formation using microtiter plate assays, as well as scanning electron and fluorescent microscopy to analyze the phenotypes of mutants lacking these proteins. In vitro and in vivo studies showed that the protease LapG of B. bronchiseptica cleaves the N-terminal domain of BrtA, as well as the LapA protein of P. fluorescens, indicating functional conservation between these species. Furthermore, while BrtA and LapG appear to have little or no impact on colonization in a mouse model of infection, a B. bronchiseptica strain lacking the LapG protease has a significantly higher rate of inducing a severe disease outcome compared to the wild type. These findings support a role for c-di-GMP acting through BrtA/LapD/LapG to modulate biofilm formation, as well as impact pathogenesis, by B. bronchiseptica
C1 [Ambrosis, Nicolas; Fernandez, Julieta; Sisti, Federico] Univ Nacl La Plata, Fac Ciencias Exactas, Dept Ciencias Biol, IBBM,CCT,CONICET La Plata, La Plata, Buenos Aires, Argentina.
[Boyd, Chelsea D.; O'Toole, George A.] Geisel Sch Med Dartmouth, Dept Microbiol & Immunol, Hanover, NH USA.
[Boyd, Chelsea D.] NIAID, Div Extramural Act, Sci Review Program, Rockville, MD USA.
RP Fernandez, J; Sisti, F (reprint author), Univ Nacl La Plata, Fac Ciencias Exactas, Dept Ciencias Biol, IBBM,CCT,CONICET La Plata, La Plata, Buenos Aires, Argentina.
EM julietafernandez3@gmail.com; federico.sisti@gmail.com
OI Sisti, Federico/0000-0001-7548-0422
FU NIH [R01-AI097307]; NSF [MCB-9984521]; CONICET; PICT [2013-0092]; PIP
[0092, 0486]; CONICET doctoral fellowship
FX This work was supported by NIH grant R01-AI097307 and NSF grant
MCB-9984521 to GAO, and International Cooperation grants (2012 and 2013)
from CONICET to FS and JF, PICT 2013-0092, PIP No 0092 and PIP No 0486
to FS and JF. NA was supported by CONICET doctoral fellowship.
NR 26
TC 1
Z9 1
U1 0
U2 3
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 5
PY 2016
VL 11
IS 7
AR e0158752
DI 10.1371/journal.pone.0158752
PG 16
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR3JE
UT WOS:000379798700044
PM 27380521
ER
PT J
AU Du, MM
Jiao, S
Bien, SA
Gala, M
Abecasis, G
Bezieau, S
Brenner, H
Butterbach, K
Caan, BJ
Carlson, CS
Casey, G
Chang-Claude, J
Conti, DV
Curtis, KR
Duggan, D
Gallinger, S
Haile, RW
Harrison, TA
Hayes, RB
Hoffmeister, M
Hopper, JL
Hudson, TJ
Jenkins, MA
Kury, S
Le Marchand, L
Leal, SM
Newcomb, PA
Nickerson, DA
Potter, JD
Schoen, RE
Schumacher, FR
Seminara, D
Slattery, ML
Hsu, L
Chan, AT
White, E
Berndt, SI
Peters, U
AF Du, Mengmeng
Jiao, Shuo
Bien, Stephanie A.
Gala, Manish
Abecasis, Goncalo
Bezieau, Stephane
Brenner, Hermann
Butterbach, Katja
Caan, Bette J.
Carlson, Christopher S.
Casey, Graham
Chang-Claude, Jenny
Conti, David V.
Curtis, Keith R.
Duggan, David
Gallinger, Steven
Haile, Robert W.
Harrison, Tabitha A.
Hayes, Richard B.
Hoffmeister, Michael
Hopper, John L.
Hudson, Thomas J.
Jenkins, Mark A.
Kury, Sebastien
Le Marchand, Loic
Leal, Suzanne M.
Newcomb, Polly A.
Nickerson, Deborah A.
Potter, John D.
Schoen, Robert E.
Schumacher, Fredrick R.
Seminara, Daniela
Slattery, Martha L.
Hsu, Li
Chan, Andrew T.
White, Emily
Berndt, Sonja I.
Peters, Ulrike
TI Fine-Mapping of Common Genetic Variants Associated with Colorectal Tumor
Risk Identified Potential Functional Variants
SO PLOS ONE
LA English
DT Article
ID GENOME-WIDE ASSOCIATION; CANCER SUSCEPTIBILITY LOCI; PROSTATE-CANCER;
GENOTYPE IMPUTATION; CHROMOSOME 8Q24; COMPLEX TRAITS; SNP RS6983267;
TARGET GENES; METAANALYSIS; EXPRESSION
AB Genome-wide association studies (GWAS) have identified many common single nucleotide polymorphisms (SNPs) associated with colorectal cancer risk. These SNPs may tag correlated variants with biological importance. Fine-mapping around GWAS loci can facilitate detection of functional candidates and additional independent risk variants. We analyzed 11,900 cases and 14,311 controls in the Genetics and Epidemiology of Colorectal Cancer Consortium and the Colon Cancer Family Registry. To fine-map genomic regions containing all known common risk variants, we imputed high-density genetic data from the 1000 Genomes Project. We tested single-variant associations with colorectal tumor risk for all variants spanning genomic regions 250-kb upstream or downstream of 31 GWAS-identified SNPs (index SNPs). We queried the University of California, Santa Cruz Genome Browser to examine evidence for biological function. Index SNPs did not show the strongest association signals with colorectal tumor risk in their respective genomic regions. Bioinformatics analysis of SNPs showing smaller P-values in each region revealed 21 functional candidates in 12 loci (5q31.1, 8q24, 11q13.4, 11q23, 12p13.32, 12q24.21, 14q22.2, 15q13, 18q21, 19q13.1, 20p12.3, and 20q13.33). We did not observe evidence of additional independent association signals in GWAS-identified regions. Our results support the utility of integrating data from comprehensive fine-mapping with expanding publicly available genomic databases to help clarify GWAS associations and identify functional candidates that warrant more onerous laboratory follow-up. Such efforts may aid the eventual discovery of disease-causing variant(s).
C1 [Du, Mengmeng] Mem Sloan Kettering Canc Ctr, Dept Epidemiol & Biostat, New York, NY 10021 USA.
[Du, Mengmeng; Jiao, Shuo; Bien, Stephanie A.; Carlson, Christopher S.; Curtis, Keith R.; Harrison, Tabitha A.; Newcomb, Polly A.; Hsu, Li; White, Emily; Peters, Ulrike] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, 1124 Columbia St, Seattle, WA 98104 USA.
[Bien, Stephanie A.; Newcomb, Polly A.; Potter, John D.; White, Emily; Peters, Ulrike] Univ Washington, Sch Publ Hlth, Seattle, WA 98195 USA.
[Gala, Manish; Chan, Andrew T.] Massachusetts Gen Hosp, Div Gastroenterol, Boston, MA 02114 USA.
[Gala, Manish; Chan, Andrew T.] Harvard Med Sch, Boston, MA USA.
[Abecasis, Goncalo] Univ Michigan, Sch Publ Hlth, Dept Biostat, Ann Arbor, MI 48109 USA.
[Bezieau, Stephane; Kury, Sebastien] CHU Nantes, Serv Genet Med, Nantes, France.
[Brenner, Hermann; Butterbach, Katja; Hoffmeister, Michael] German Canc Res Ctr, Div Clin Epidemiol & Aging Res, Heidelberg, Germany.
[Brenner, Hermann] German Canc Consortium DKTK, Heidelberg, Germany.
[Caan, Bette J.] Kaiser Permanente Med Care Program Northern Calif, Div Res, Oakland, CA USA.
[Casey, Graham; Conti, David V.; Haile, Robert W.; Schumacher, Fredrick R.] Univ Southern Calif, Keck Sch Med, Los Angeles, CA USA.
[Chang-Claude, Jenny] German Canc Res Ctr, Div Canc Epidemiol, Heidelberg, Germany.
[Duggan, David] Translat Genom Res Inst, Phoenix, AZ USA.
[Gallinger, Steven] Mt Sinai Hosp, Dept Surg, Toronto, ON, Canada.
[Hayes, Richard B.] NYU, Sch Med, Dept Populat Hlth, Div Epidemiol, New York, NY USA.
[Hopper, John L.; Jenkins, Mark A.] Univ Melbourne, Melbourne Sch Populat & Global Hlth, Melbourne, Vic, Australia.
[Hudson, Thomas J.] Ontario Inst Canc Res, Toronto, ON, Canada.
[Hudson, Thomas J.] Univ Toronto, Dept Med Biophys, Toronto, ON, Canada.
[Hudson, Thomas J.] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada.
[Le Marchand, Loic] Univ Hawaii, Ctr Canc, Program Epidemiol, Honolulu, HI 96822 USA.
[Leal, Suzanne M.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA.
[Nickerson, Deborah A.] Univ Washington, Genome Sci, Seattle, WA 98195 USA.
[Potter, John D.] Massey Univ, Ctr Publ Hlth Res, Wellington, New Zealand.
[Schoen, Robert E.] Univ Pittsburgh, Med Ctr, Dept Med & Epidemiol, Pittsburgh, PA USA.
[Seminara, Daniela] NCI, Div Canc Control & Populat Sci, Bethesda, MD 20892 USA.
[Slattery, Martha L.] Univ Utah, Hlth Sci Ctr, Dept Internal Med, Salt Lake City, UT USA.
[Chan, Andrew T.] Brigham & Womens Hosp, Channing Div Network Med, 75 Francis St, Boston, MA 02115 USA.
[Berndt, Sonja I.] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA.
RP Du, MM (reprint author), Mem Sloan Kettering Canc Ctr, Dept Epidemiol & Biostat, New York, NY 10021 USA.; Du, MM; Peters, U (reprint author), Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, 1124 Columbia St, Seattle, WA 98104 USA.; Peters, U (reprint author), Univ Washington, Sch Publ Hlth, Seattle, WA 98195 USA.
EM dumeng@mskcc.org; upeters@fhcrc.org
RI Jenkins, Mark/P-7803-2015; Gallinger, Steven/E-4575-2013; Brenner,
Hermann/B-4627-2017;
OI Jenkins, Mark/0000-0002-8964-6160; Brenner, Hermann/0000-0002-6129-1572;
Hayes, Richard/0000-0002-0918-661X; Bien, Stephanie/0000-0002-8713-4583;
Hoffmeister, Michael/0000-0002-8307-3197
FU GECCO: National Cancer Institute; GECCO: National Institutes of Health;
GECCO: U.S. Department of Health and Human Services [U01 CA137088, R01
CA059045, U01 CA164930]; National Cancer Institute [R25 CA94880, P30
CA008748]; Regional Council of Pays de la Loire; Groupement des
Entreprises Francaises dans la Lutte contre le Cancer (GEFLUC);
Association Anne de Bretagne Genetique; Ligue Regionale Contre le Cancer
(LRCC); National Cancer Institute, National Institutes of Health [U01
CA122839]; National Institutes of Health: Australasian Colorectal Cancer
Family Registry [U01 CA097735]; National Institutes of Health: Ontario
Registry for Studies of Familial Colorectal Cancer [U01 CA074783];
National Institutes of Health: Seattle Colorectal Cancer Family Registry
[U01 CA074794]; DACHS: German Research Council (Deutsche
Forschungsgemeinschaft) [BR 1704/6-1, BR 1704/6-3, BR 1704/6-4, CH
117/1-1]; COLO2&3: National Institutes of Health [R01 CA60987]; CCFR:
National Institutes of Health (RFA) [CA-95-011]; German Federal Ministry
of Education and Research [01KH0404, 01ER0814]; DALS: National
Institutes of Health [R01 CA48998]; NHS; PHS: HPFS by National
Institutes of Health [P01 CA 055075, UM1 CA167552, R01 137178, P50 CA
127003]; NHS by the National Institutes of Health [P50 CA 127003, R01
CA137178, P01 CA 087969]; National Institutes of Health [CA42182]; Damon
Runyon Clinical investigator Award; MEC: National Institutes of Health
[R37 CA54281, P01 CA033619, R01 CA63464]; OFCCR: National Institutes of
Health [U01 CA074783]; GL2 grant from Ontario Research Fund; Canadian
Institutes of Health Research; Cancer Risk Evaluation (CaRE) Program
grant from Canadian Cancer Society Research Institute; Senior
Investigator Awards from Ontario Institute for Cancer Research; Ontario
Ministry of Research and Innovation; PLCO: Intramural Research Program
of the Division of Cancer Epidemiology and Genetics; Division of Cancer
Prevention, National Cancer Institute, NIH, DHHS; National Institutes of
Health (NIH), Genes, Environment and Health Initiative (GEI) [Z01 CP
010200]; NIH [U01 HG004446]; NIH GEI [U01 HG 004438]; PMH-CCFR: National
Institutes of Health [R01 CA076366]; VITAL: National Institutes of
Health [K05 CA154337]; National Heart, Lung, and Blood Institute,
National Institutes of Health, U.S. Department of Health and Human
Services [HHSN268201100046C, HHSN268201100001C, HHSN268201100002C,
HHSN268201100003C, HHSN268201100004C, HHSN271201100004C]; CORECT:
National Cancer Institute, National Institutes of Health under RFA
[CA-09-002 (U19 CA148107)]; [K24 DK098311]
FX This work was supported by the following: GECCO: National Cancer
Institute, National Institutes of Health, U.S. Department of Health and
Human Services (U01 CA137088; R01 CA059045; U01 CA164930), M.D. is
supported by grants R25 CA94880 and P30 CA008748 from the National
Cancer Institute. ASTERISK: Hospital Clinical Research Program (PHRC)
and supported by the Regional Council of Pays de la Loire, the
Groupement des Entreprises Francaises dans la Lutte contre le Cancer
(GEFLUC), the Association Anne de Bretagne Genetique and the Ligue
Regionale Contre le Cancer (LRCC). COLO2&3: National Institutes of
Health (R01 CA60987). CCFR: National Institutes of Health (RFA #
CA-95-011) and through cooperative agreements with members of the Colon
Cancer Family Registry and P.I.s. This genome wide scan was supported by
the National Cancer Institute, National Institutes of Health by U01
CA122839. The content of this manuscript does not necessarily reflect
the views or policies of the National Cancer Institute or any of the
collaborating centers in the CFRs, nor does mention of trade names,
commercial products, or organizations imply endorsement by the US
Government or the CFR. The following Colon CFR centers contributed data
to this manuscript and were supported by National Institutes of Health:
Australasian Colorectal Cancer Family Registry (U01 CA097735), Ontario
Registry for Studies of Familial Colorectal Cancer (U01 CA074783), and
Seattle Colorectal Cancer Family Registry (U01 CA074794). DACHS: German
Research Council (Deutsche Forschungsgemeinschaft, BR 1704/6-1, BR
1704/6-3, BR 1704/6-4 and CH 117/1-1), and the German Federal Ministry
of Education and Research (01KH0404 and 01ER0814). DALS: National
Institutes of Health (R01 CA48998 to M.L.S.); HPFS, NHS, and PHS: HPFS
by the National Institutes of Health (P01 CA 055075, UM1 CA167552, R01
137178, and P50 CA 127003), NHS by the National Institutes of Health
(R01 CA137178, P01 CA 087969 and P50 CA 127003,) and PHS by the National
Institutes of Health (CA42182). A.T.C. is also supported by a Damon
Runyon Clinical investigator Award and K24 DK098311. MEC: National
Institutes of Health (R37 CA54281, P01 CA033619, and R01 CA63464).
OFCCR: National Institutes of Health, through funding allocated to the
Ontario Registry for Studies of Familial Colorectal Cancer (U01
CA074783); see CCFR section above. As subset of ARCTIC, OFCCR is
supported by a GL2 grant from the Ontario Research Fund, the Canadian
Institutes of Health Research, and the Cancer Risk Evaluation (CaRE)
Program grant from the Canadian Cancer Society Research Institute.
T.J.H. is a recipient of Senior Investigator Awards from the Ontario
Institute for Cancer Research, through generous support from the Ontario
Ministry of Research and Innovation. PLCO: Intramural Research Program
of the Division of Cancer Epidemiology and Genetics and supported by
contracts from the Division of Cancer Prevention, National Cancer
Institute, NIH, DHHS. Additionally, a subset of control samples were
genotyped as part of the Cancer Genetic Markers of Susceptibility
(CGEMS) Prostate Cancer GWAS (Yeager, M et al. Nat Genet 2007
May;39(5):645-9), Colon CGEMS pancreatic cancer scan (PanScan)
(Amundadottir, L et al. Nat Genet. 2009 Sep;41(9):986-90 and Petersen,
GM et al Nat Genet. 2010 Mar;42(3):2248), and the Lung Cancer and
Smoking study. The prostate and PanScan study datasets were accessed
with appropriate approval through the dbGaP online resource
(http://cgems.cancer.gov/data/) accession numbers phs000207v.1p1 and
phs000206.v3.; p2, respectively, and the lung datasets were accessed
from the dbGaP website (http://www.ncbi.nlm.nih.gov/gap) through
accession number phs000093v2.p2. Funding for the Lung Cancer and Smoking
study was provided by National Institutes of Health (NIH), Genes,
Environment and Health Initiative (GEI) Z01 CP 010200, NIH U01 HG004446,
and NIH GEI U01 HG 004438. For the lung study, the GENEVA Coordinating
Center provided assistance with genotype cleaning and general study
coordination, and the Johns Hopkins University Center for Inherited
Disease Research conducted genotyping. PMH-CCFR: National Institutes of
Health (R01 CA076366 to P.A.N.). VITAL: National Institutes of Health
(K05 CA154337). WHI: The WHI program is funded by the National Heart,
Lung, and Blood Institute, National Institutes of Health, U.S.
Department of Health and Human Services through contracts
HHSN268201100046C, HHSN268201100001C, HHSN268201100002C,
HHSN268201100003C, HHSN268201100004C, and HHSN271201100004C. CORECT:
National Cancer Institute, National Institutes of Health under RFA
#CA-09-002 (U19 CA148107). The content of this manuscript does not
necessarily reflect the views or policies of the National Cancer
Institute or any of the collaborating centers in CORECT, nor does
mention of trade names, commercial products, or organizations imply
endorsement by the US Government or CORECT. The funders had no role in
study design, data collection and analysis, decision to publish, or
preparation of the manuscript.
NR 75
TC 1
Z9 1
U1 3
U2 8
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1932-6203
J9 PLOS ONE
JI PLoS One
PD JUL 5
PY 2016
VL 11
IS 7
AR e0157521
DI 10.1371/journal.pone.0157521
PG 18
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DR3JE
UT WOS:000379798700007
PM 27379672
ER
PT J
AU Wilson, LE
D'Aloisio, AA
Sandler, DP
Taylor, JA
AF Wilson, Lauren E.
D'Aloisio, Aimee A.
Sandler, Dale P.
Taylor, Jack A.
TI Long-term use of calcium channel blocking drugs and breast cancer risk
in a prospective cohort of US and Puerto Rican women
SO BREAST CANCER RESEARCH
LA English
DT Article
DE Calcium channel blockers; Antihypertensive drugs; Breast cancer risk
ID ANTIHYPERTENSIVE MEDICATION USE; CELL-LINES; IN-VITRO; BLOCKERS;
APOPTOSIS; VERAPAMIL; HYPERTENSION; ASSOCIATION; MORTALITY; CARCINOMA
AB Background: In a recent case-control study, long-term use of calcium channel blocking drugs was associated with a greater-than-twofold increased breast cancer risk. If prospectively collected data confirm that calcium channel blocker use increases breast cancer risk, this would have major implications for hypertension treatment. The objective of this study was to determine whether women using calcium channel blockers for 10 years or more were at increased risk of developing breast cancer compared with women not using calcium channel blockers.
Methods: The Sister Study is a prospective volunteer cohort study of women from the USA and Puerto Rico designed to evaluate environmental and genetic risk factors for breast cancer. Beginning in 2003, women between the ages of 35 and 74 were recruited. They were eligible to participate if they had a sister with breast cancer but had not been diagnosed with breast cancer themselves. In total, 50,884 women enrolled in the cohort between 2003 and 2009; 50,757 women with relevant baseline data and available follow-up data are included in this study. The exposure of interest is current use of calcium channel blocking drugs and the reported duration of use at entry into the cohort. Secondary exposures of interest were the duration and frequency of use for all other subclasses of antihypertensive drugs. Our main outcome is a self-reported diagnosis of breast cancer during the study follow-up period. With patient permission, self-reported diagnoses were confirmed using medical records.
Results: Results showed 15,817 participants were currently using an antihypertensive drug, and 3316 women were currently using a calcium channel blocker at study baseline; 1965 women reported a breast cancer diagnosis during study follow-up. Using Cox proportional hazards modeling, we found no increased risk of breast cancer among women who had been using calcium channel blockers for 10 years or more compared with never users of calcium channel blockers (HR 0.88, 95 % CI 0.58-1.33).
Conclusions: We saw no evidence of increased risk of breast cancer from 10 years or more of current calcium channel blocker use. Our results do not support avoiding calcium channel blocking drugs in order to reduce breast cancer risk.
C1 [Wilson, Lauren E.; D'Aloisio, Aimee A.; Sandler, Dale P.; Taylor, Jack A.] NIEHS, Epidemiol Branch, NIH, Res Triangle Pk, NC 27709 USA.
[D'Aloisio, Aimee A.] Social & Sci Syst Inc, Durham, NC 27703 USA.
RP Taylor, JA (reprint author), NIEHS, Epidemiol Branch, NIH, Res Triangle Pk, NC 27709 USA.
EM taylor@niehs.nih.gov
OI taylor, jack/0000-0001-5303-6398; Sandler, Dale/0000-0002-6776-0018;
Wilson, Lauren/0000-0002-5953-2293
FU Intramural Research Program of the National Institute of Environmental
Health Sciences, NIH [Z01 ES044005, Z01 ES044032, Z01 ES049033]
FX This research was supported by the Intramural Research Program of the
National Institute of Environmental Health Sciences, NIH (Z01 ES044005,
Z01 ES044032, and Z01 ES049033), which provided funding for design and
conduct of the study; collection, management, analysis, and
interpretation of the data; and preparation, review, and approval of the
manuscript.
NR 33
TC 3
Z9 3
U1 1
U2 6
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1465-542X
EI 1465-5411
J9 BREAST CANCER RES
JI Breast Cancer Res.
PD JUL 5
PY 2016
VL 18
AR 61
DI 10.1186/s13058-016-0720-6
PG 8
WC Oncology
SC Oncology
GA DQ6KN
UT WOS:000379313800001
PM 27378129
ER
PT J
AU Pauli, R
Bowring, A
Reynolds, R
Chen, G
Nichols, TE
Maumet, C
AF Pauli, Ruth
Bowring, Alexander
Reynolds, Richard
Chen, Gang
Nichols, Thomas E.
Maumet, Camille
TI Exploring fMRI Results Space: 31 Variants of an fMRI Analysis in AFNI,
FSL, and SPM
SO FRONTIERS IN NEUROINFORMATICS
LA English
DT Article
DE data sharing; functional MRI; provenance; fMRI analysis; neuroimaging
ID PROJECT
C1 [Pauli, Ruth; Bowring, Alexander; Nichols, Thomas E.; Maumet, Camille] Univ Warwick, Warwick Mfg Grp, Coventry, W Midlands, England.
[Reynolds, Richard; Chen, Gang] NIMH, Sci & Stat Comp Core, NIH, Bethesda, MD 20892 USA.
[Nichols, Thomas E.] Univ Warwick, Dept Stat, Coventry, W Midlands, England.
RP Pauli, R (reprint author), Univ Warwick, Warwick Mfg Grp, Coventry, W Midlands, England.
EM r.pauli@warwick.ac.uk
FU Git Large File Storage (Git-LFS); Welcome Trust; BBSRC; NIMH intramural
Research Programs of the NIH/HHS, USA [ZICMH002888]; NINDS intramural
Research Programs of the NIH/HHS, USA [ZICMH002888]
FX The INCF provided funding for the use of Git Large File Storage
(Git-LFS). AB, CM, and TN were supported by the Welcome Trust. RP was
supported by the BBSRC-funded Midlands Integrative Biosciences Training
Partnership (MIBTP), and RR and GC were supported by the NIMH and NINDS
intramural Research Programs (ZICMH002888) of the NIH/HHS, USA.
NR 14
TC 1
Z9 1
U1 4
U2 4
PU FRONTIERS MEDIA SA
PI LAUSANNE
PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015,
SWITZERLAND
SN 1662-5196
J9 FRONT NEUROINFORM
JI Front. Neuroinformatics
PD JUL 5
PY 2016
VL 10
AR 24
DI 10.3389/fninf.2016.00024
PG 6
WC Mathematical & Computational Biology; Neurosciences
SC Mathematical & Computational Biology; Neurosciences & Neurology
GA DQ3FU
UT WOS:000379089000001
PM 27458367
ER
PT J
AU Hripcsak, G
Ryan, PB
Duke, JD
Shah, NH
Park, RW
Huser, V
Suchard, MA
Schuemie, MJ
DeFalco, FJ
Perotte, A
Banda, JM
Reich, CG
Schilling, LM
Matheny, ME
Meeker, D
Pratt, N
Madigan, D
AF Hripcsak, George
Ryan, Patrick B.
Duke, Jon D.
Shah, Nigam H.
Park, Rae Woong
Huser, Vojtech
Suchard, Marc A.
Schuemie, Martijn J.
DeFalco, Frank J.
Perotte, Adler
Banda, Juan M.
Reich, Christian G.
Schilling, Lisa M.
Matheny, Michael E.
Meeker, Daniella
Pratt, Nicole
Madigan, David
TI Characterizing treatment pathways at scale using the OHDSI network
SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
LA English
DT Article
DE observational research; data network; treatment pathways
ID CONTROLLED CASE SERIES; CLINICAL PATHWAYS; CONTROLLED-TRIALS;
TASK-FORCE; HEALTH; MEDICATION; OUTCOMES; QUALITY; TIME; RECOMMENDATIONS
AB Observational research promises to complement experimental research by providing large, diverse populations that would be infeasible for an experiment. Observational research can test its own clinical hypotheses, and observational studies also can contribute to the design of experiments and inform the generalizability of experimental research. Understanding the diversity of populations and the variance in care is one component. In this study, the Observational Health Data Sciences and Informatics (OHDSI) collaboration created an international data network with 11 data sources from four countries, including electronic health records and administrative claims data on 250 million patients. All data were mapped to common data standards, patient privacy was maintained by using a distributed model, and results were aggregated centrally. Treatment pathways were elucidated for type 2 diabetes mellitus, hypertension, and depression. The pathways revealed that the world is moving toward more consistent therapy over time across diseases and across locations, but significant heterogeneity remains among sources, pointing to challenges in generalizing clinical trial results. Diabetes favored a single first-line medication, metformin, to a much greater extent than hypertension or depression. About 10% of diabetes and depression patients and almost 25% of hypertension patients followed a treatment pathway that was unique within the cohort. Aside from factors such as sample size and underlying population (academic medical center versus general population), electronic health records data and administrative claims data revealed similar results. Large-scale international observational research is feasible.
C1 [Hripcsak, George; Perotte, Adler] Columbia Univ, Med Ctr, Dept Biomed Informat, New York, NY 10032 USA.
[Hripcsak, George] NewYork Presbyterian Hosp, Med Informat Serv, New York, NY 10032 USA.
[Hripcsak, George; Ryan, Patrick B.; Duke, Jon D.; Shah, Nigam H.; Park, Rae Woong; Huser, Vojtech; Suchard, Marc A.; Schuemie, Martijn J.; DeFalco, Frank J.; Perotte, Adler; Banda, Juan M.; Reich, Christian G.; Schilling, Lisa M.; Matheny, Michael E.; Meeker, Daniella; Pratt, Nicole; Madigan, David] Observat Hlth Data Sci & Informat, New York, NY 10032 USA.
[Ryan, Patrick B.; Schuemie, Martijn J.; DeFalco, Frank J.] Janssen Res & Dev, Epidemiol Analyt, Titusville, NJ 08560 USA.
[Duke, Jon D.] Regenstrief Inst Hlth Care, Ctr Biomed Informat, Indianapolis, IN 46205 USA.
[Shah, Nigam H.; Banda, Juan M.] Stanford Univ, Ctr Biomed Informat Res, Stanford, CA 94305 USA.
[Park, Rae Woong] Ajou Univ, Sch Med, Dept Biomed Informat, Suwon 443380, South Korea.
[Huser, Vojtech] NIH, Lister Hill Natl Ctr Biomed Commun, Natl Lib Med, Bethesda, MD 20894 USA.
[Suchard, Marc A.] Univ Calif Los Angeles, Dept Biomath, Los Angeles, CA 90095 USA.
[Suchard, Marc A.] Univ Calif Los Angeles, Dept Biostat, Los Angeles, CA 90095 USA.
[Suchard, Marc A.] Univ Calif Los Angeles, Dept Human Genet, Los Angeles, CA 90095 USA.
[Reich, Christian G.] IMS Hlth, Real World Evidence Solut, Burlington, MA 01809 USA.
[Schilling, Lisa M.] Univ Colorado, Sch Med, Dept Med, Aurora, CO 80045 USA.
[Matheny, Michael E.] Vanderbilt Univ, Med Ctr, Dept Biomed Informat, Nashville, TN 37212 USA.
[Matheny, Michael E.] VA Tennessee Valley Healthcare Syst, Geriatr Res Educ & Clin Ctr, Nashville, TN 37212 USA.
[Meeker, Daniella] Univ So Calif, Dept Prevent Med, Los Angeles, CA 90089 USA.
[Meeker, Daniella] Univ So Calif, Dept Pediat, Los Angeles, CA 90089 USA.
[Pratt, Nicole] Univ S Australia, Div Hlth Sci, Adelaide, SA 5001, Australia.
[Madigan, David] Columbia Univ, Dept Stat, New York, NY 10027 USA.
RP Hripcsak, G (reprint author), Columbia Univ, Med Ctr, Dept Biomed Informat, New York, NY 10032 USA.; Hripcsak, G (reprint author), NewYork Presbyterian Hosp, Med Informat Serv, New York, NY 10032 USA.; Hripcsak, G (reprint author), Observat Hlth Data Sci & Informat, New York, NY 10032 USA.
EM hripcsak@columbia.edu
OI Banda, Juan/0000-0001-8499-824X
FU National Library of Medicine [R01 LM006910, R01 LM011369]; National
Institute of General Medical Sciences [R01 GM101430]; National Science
Foundation [NSF IIS 1251151]; Smart Family Foundation; Janssen Research
and Development; AstraZeneca; Takeda Pharmaceuticals International
FX This work was funded in part by Grants R01 LM006910 and R01 LM011369
from the National Library of Medicine, Grant R01 GM101430 from the
National Institute of General Medical Sciences, Grant NSF IIS 1251151
from the National Science Foundation, and by the Smart Family
Foundation. Infrastructure to carry out the project was funded in part
by Janssen Research and Development, AstraZeneca, and Takeda
Pharmaceuticals International. Use of the CPRD data set was approved by
the CPRD Independent Scientific Advisory Committee as protocol 15_019R.
NR 39
TC 4
Z9 4
U1 0
U2 1
PU NATL ACAD SCIENCES
PI WASHINGTON
PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
SN 0027-8424
J9 P NATL ACAD SCI USA
JI Proc. Natl. Acad. Sci. U. S. A.
PD JUL 5
PY 2016
VL 113
IS 27
BP 7329
EP 7336
DI 10.1073/pnas.1510502113
PG 8
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ2GW
UT WOS:000379021700036
PM 27274072
ER
PT J
AU Kasbekara, M
Fischer, G
Mott, BT
Yasgar, A
Hyvonen, M
Boshoff, HIM
Abell, C
Barry, CE
Thomas, CJ
AF Kasbekar, Monica
Fischer, Gerhard
Mott, Bryan T.
Yasgar, Adam
Hyvonen, Marko
Boshoff, Helena I. M.
Abell, Chris
Barry, Clifton E., III
Thomas, Craig J.
TI Selective small molecule inhibitor of the Mycobacterium tuberculosis
fumarate hydratase reveals an allosteric regulatory site
SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
LA English
DT Article
DE allosteric regulation; selective inhibition; fumarate hydratase;
Mycobacterium tuberculosis; TCA cycle
ID ESCHERICHIA-COLI; MOONLIGHTING PROTEINS; METABOLISM; LIBRARIES;
PARADIGM; BINDING; ENZYME; ASSAYS; GENE
AB Enzymes in essential metabolic pathways are attractive targets for the treatment of bacterial diseases, but in many cases, the presence of homologous human enzymes makes them impractical candidates for drug development. Fumarate hydratase, an essential enzyme in the tricarboxylic acid (TCA) cycle, has been identified as one such potential therapeutic target in tuberculosis. We report the discovery of the first small molecule inhibitor, to our knowledge, of the Mycobacterium tuberculosis fumarate hydratase. A crystal structure at 2.0-angstrom resolution of the compound in complex with the protein establishes the existence of a previously unidentified allosteric regulatory site. This allosteric site allows for selective inhibition with respect to the homologous human enzyme. We observe a unique binding mode in which two inhibitor molecules interact within the allosteric site, driving significant conformational changes that preclude simultaneous substrate and inhibitor binding. Our results demonstrate the selective inhibition of a highly conserved metabolic enzyme that contains identical active site residues in both the host and the pathogen.
C1 [Kasbekar, Monica; Mott, Bryan T.; Yasgar, Adam; Thomas, Craig J.] NIH, Natl Ctr Adv Translat Sci, Bethesda, MD 20850 USA.
[Kasbekar, Monica; Abell, Chris] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England.
[Fischer, Gerhard; Hyvonen, Marko] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England.
[Boshoff, Helena I. M.; Barry, Clifton E., III] NIAID, NIH, Bethesda, MD 20850 USA.
RP Thomas, CJ (reprint author), NIH, Natl Ctr Adv Translat Sci, Bethesda, MD 20850 USA.
EM craigt@mail.nih.gov
OI Fischer, Gerhard/0000-0001-9861-1528
FU National Institutes of Health-Oxford-Cambridge Scholars Program;
Washington University in St. Louis Medical Scientist Training Program;
Division of Preclinical Innovation, National Center for Advancing
Translational Sciences; Intramural Research Program of the National
Institute of Allergy and Infectious Diseases
FX We thank Dr. Christina Spry, Dr. David Garboczi, Dr. Apostolos Gittis,
Dr. Paresma Patel, and Dr. Tathagata Mukherjee for helpful advice and
discussions. We also thank Sam Michael, Charles Bonney, Dr. Rajarshi
Guha, and Paul Shinn for help with the high-throughput screen and
compound management. Some experiments were performed in the
Crystallographic X-Ray Facility at the Department of Biochemistry,
University of Cambridge. We thank Dr. Dimitri Chirgadze for his
assistance in using these facilities. We also thank Diamond Light Source
and staff for the provision of beam time at beamlines I04 and I24. M.K.
is supported by the National Institutes of Health-Oxford-Cambridge
Scholars Program and the Washington University in St. Louis Medical
Scientist Training Program. This work was supported by the Division of
Preclinical Innovation, National Center for Advancing Translational
Sciences and the Intramural Research Program of the National Institute
of Allergy and Infectious Diseases.
NR 35
TC 3
Z9 3
U1 3
U2 8
PU NATL ACAD SCIENCES
PI WASHINGTON
PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
SN 0027-8424
J9 P NATL ACAD SCI USA
JI Proc. Natl. Acad. Sci. U. S. A.
PD JUL 5
PY 2016
VL 113
IS 27
BP 7503
EP 7508
DI 10.1073/pnas.1600630113
PG 6
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ2GW
UT WOS:000379021700064
PM 27325754
ER
PT J
AU Zhang, Y
Yokoyama, S
Herriges, JC
Zhang, Z
Young, RE
Verheyden, JM
Sun, X
AF Zhang, Yan
Yokoyama, Shigetoshi
Herriges, John C.
Zhang, Zhen
Young, Randee E.
Verheyden, Jamie M.
Sun, Xin
TI E3 ubiquitin ligase RFWD2 controls lung branching through protein-level
regulation of ETV transcription factors
SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
LA English
DT Article
DE lung branching; RFWD2; COP1; ETV transcription factors; E3 ubiquitin
ligases
ID HORMONE-RELATED PROTEIN; MOUSE LUNG; NEGATIVE REGULATOR;
TUMOR-SUPPRESSOR; MULTIPLE ROLES; C/EBP-ALPHA; COP1; MORPHOGENESIS;
DEGRADATION; EXPRESSION
AB The mammalian lung is an elaborate branching organ, and it forms following a highly stereotypical morphogenesis program. It is well established that precise control at the transcript level is a key genetic underpinning of lung branching. In comparison, little is known about how regulation at the protein level may play a role. Ring finger and WD domain 2 (RFWD2, also termed COP1) is an E3 ubiquitin ligase that modifies specific target proteins, priming their degradation via the ubiquitin proteasome system. RFWD2 is known to function in the adult in pathogenic processes such as tumorigenesis. Here, we show that prenatal inactivation of Rfwd2 gene in the lung epithelium led to a striking halt in branching morphogenesis shortly after secondary branch formation. This defect is accompanied by distalization of the lung epithelium while growth and cellular differentiation still occurred. In the mutant lung, two E26 transformation-specific (ETS) transcription factors essential for normal lung branching, ETS translocation variant 4 (ETV4) and ETV5, were up-regulated at the protein level, but not at the transcript level. Introduction of Etv loss-of-function alleles into the Rfwd2 mutant background attenuated the branching phenotype, suggesting that RFWD2 functions, at least in part, through degrading ETV proteins. Because a number of E3 ligases are known to target factors important for lung development, our findings provide a preview of protein-level regulatory network essential for lung branching morphogenesis.
C1 [Zhang, Yan; Yokoyama, Shigetoshi; Herriges, John C.; Zhang, Zhen; Young, Randee E.; Verheyden, Jamie M.; Sun, Xin] Univ Wisconsin, Dept Med Genet, Lab Genet, Madison, WI 53706 USA.
[Yokoyama, Shigetoshi] Natl Canc Inst, Lab Metab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
[Zhang, Zhen] NGM Biopharmaceut Inc, San Francisco, CA 94080 USA.
RP Sun, X (reprint author), Univ Wisconsin, Dept Med Genet, Lab Genet, Madison, WI 53706 USA.
EM xsun@wisc.edu
RI yokoyama, shigetoshi/S-5912-2016
OI yokoyama, shigetoshi/0000-0003-4175-0548
FU American Heart Association [15POST25670070]; National Heart, Lung, and
Blood Institute [RO1 HL113870, HL097134]; Wisconsin Partnership Program
[2897]
FX We thank members of the X.S. laboratory for constructive discussions and
readings of the manuscript, Dr. Vishva M. Dixit (Genentech) for sharing
the Rfwd2fl mice, and Dr. Scott Berry and Dr. David Beebe
(University of Wisconsin-Madison) for guidance on immunoprecipitation
experiments. This work was supported by American Heart Association
Postdoctoral Fellowship 15POST25670070 (to Y.Z.) and by National Heart,
Lung, and Blood Institute Grants RO1 HL113870 and HL097134 and Wisconsin
Partnership Program Grant 2897 (to X.S.).
NR 55
TC 0
Z9 0
U1 7
U2 10
PU NATL ACAD SCIENCES
PI WASHINGTON
PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
SN 0027-8424
J9 P NATL ACAD SCI USA
JI Proc. Natl. Acad. Sci. U. S. A.
PD JUL 5
PY 2016
VL 113
IS 27
BP 7557
EP 7562
DI 10.1073/pnas.1603310113
PG 6
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ2GW
UT WOS:000379021700073
PM 27335464
ER
PT J
AU Li, QS
Sodroski, C
Lowey, B
Schweitzer, CJ
Cha, HL
Zhang, F
Liang, TJ
AF Li, Qisheng
Sodroski, Catherine
Lowey, Brianna
Schweitzer, Cameron J.
Cha, Helen
Zhang, Fang
Liang, T. Jake
TI Hepatitis C virus depends on E-cadherin as an entry factor and regulates
its expression in epithelial-to-mesenchymal transition
SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
AMERICA
LA English
DT Article
DE hepatitis C virus; viral entry; E-cadherin; tight junction;
epithelial-mesenchymal transition
ID B TYPE-I; PRIMARY HUMAN HEPATOCYTES; LIFE-CYCLE;
HEPATOCELLULAR-CARCINOMA; SUPERINFECTION EXCLUSION; CORE-PROTEIN;
CELL-CULTURE; HOST FACTORS; INFECTION; RECEPTOR
AB Hepatitis C virus (HCV) enters the host cell through interactions with a cascade of cellular factors. Although significant progress has been made in understanding HCV entry, the precise mechanisms by which HCV exploits the receptor complex and host machinery to enter the cell remain unclear. This intricate process of viral entry likely depends on additional yet-to-be-defined cellular molecules. Recently, by applying integrative functional genomics approaches, we identified and interrogated distinct sets of host dependencies in the complete HCV life cycle. Viral entry assays using HCV pseudoparticles (HCVpps) of various genotypes uncovered multiple previously unappreciated host factors, including E-cadherin, that mediate HCV entry. E-cadherin silencing significantly inhibited HCV infection in Huh7.5.1 cells, HepG2/miR122/CD81 cells, and primary human hepatocytes at a postbinding entry step. Knockdown of E-cadherin, however, had no effect on HCV RNA replication or internal ribosomal entry site (IRES)-mediated translation. In addition, an E-cadherin monoclonal antibody effectively blocked HCV entry and infection in hepatocytes. Mechanistic studies demonstrated that E-cadherin is closely associated with claudin-1 (CLDN1) and occludin (OCLN) on the cell membrane. Depletion of E-cadherin drastically diminished the cell-surface distribution of these two tight junction proteins in various hepatic cell lines, indicating that E-cadherin plays an important regulatory role in CLDN1/OCLN localization on the cell surface. Furthermore, loss of E-cadherin expression in hepatocytes is associated with HCV-induced epithelial-to-mesenchymal transition (EMT), providing an important link between HCV infection and liver cancer. Our data indicate that a dynamic interplay among E-cadherin, tight junctions, and EMT exists and mediates an important function in HCV entry.
C1 [Li, Qisheng; Sodroski, Catherine; Lowey, Brianna; Schweitzer, Cameron J.; Cha, Helen; Zhang, Fang; Liang, T. Jake] NIDDK, Liver Dis Branch, NIH, Bethesda, MD 20892 USA.
RP Li, QS; Liang, TJ (reprint author), NIDDK, Liver Dis Branch, NIH, Bethesda, MD 20892 USA.
EM liqisheng@niddk.nih.gov; jakel@bdg10.niddk.nih.gov
FU Intramural Research Program of the National Institute of Diabetes and
Digestive and Kidney Diseases, National Institutes of Health
FX We thank Ching-Sheng Hsu and Veronique Pene for technical assistance;
Francis Chisari, Charles Rice, Takaji Wakita, Francois-Loic Cosset,
Michael Niepmann, Jens Bukh, Tetsuro Suzuki, and Takanobu Kato for their
generosity in providing reagents; and all members of the Liver Diseases
Branch for discussion and support. This work was supported by the
Intramural Research Program of the National Institute of Diabetes and
Digestive and Kidney Diseases, National Institutes of Health.
NR 52
TC 3
Z9 3
U1 3
U2 9
PU NATL ACAD SCIENCES
PI WASHINGTON
PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
SN 0027-8424
J9 P NATL ACAD SCI USA
JI Proc. Natl. Acad. Sci. U. S. A.
PD JUL 5
PY 2016
VL 113
IS 27
BP 7620
EP 7625
DI 10.1073/pnas.1602701113
PG 6
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ2GW
UT WOS:000379021700084
PM 27298373
ER
PT J
AU Davoli-Ferreira, M
Fonseca, DM
Mota, CM
Dias, MS
Lima, DS
da Silva, MV
Quirino, GFS
Zamboni, DS
Silva, JS
Mineo, TWP
AF Davoli-Ferreira, Marcela
Fonseca, Denise M.
Mota, Caroline M.
Dias, Murilo S.
Lima-Junior, Djalma S.
da Silva, Murilo V.
Quirino, Gustavo F. S.
Zamboni, Dario S.
Silva, Joao S.
Mineo, Tiago W. P.
TI Nucleotide-binding oligomerization domain-containing protein 2 prompts
potent inflammatory stimuli during Neospora caninum infection
SO SCIENTIFIC REPORTS
LA English
DT Article
ID TOLL-LIKE RECEPTORS; IMMUNE-RESPONSES; HOST-DEFENSE; KAPPA-B; NOD2;
DISEASE; INNATE; ACTIVATION; CELLS; PEPTIDOGLYCAN
AB Neospora caninum is an apicomplexan parasite responsible for major economic losses due to abortions in cattle. Innate immune responses are crucial for host resistance against the infection, however the molecules involved in parasite recognition are still poorly understood. Nod2 is a cytosolic receptor that recognizes several pathogens and its role during N. caninum infection has not yet been described. In that sense, we evaluated the role of Nod2 in host response against this parasite. We found that infection of macrophages induced increased expression of Nod2, which colocalized with the parasites' vacuoles. Nod2-deficient macrophages showed an impaired induction of pro-inflammatory cytokines, increased production of modulatory molecules, and failure to restrict parasite replication. In vivo, Nod2-knockout mice showed a reduction of MAPK phosphorylation and proinflammatory cytokines, followed by decreased inflammation in target organs and increment in parasite burden. Surprisingly, these mice were partially resistant to lethal doses of tachyzoites. In addition, these phenomena were not observed in Rip2-/- mice. In conclusion, our study indicates that Nod2-dependent responses account for N. caninum elimination. On the other hand, the inflammatory milieu induced by this innate receptor provoked pathogenesis and death in severe experimental neosporosis.
C1 [Davoli-Ferreira, Marcela; Mota, Caroline M.; da Silva, Murilo V.; Mineo, Tiago W. P.] Univ Fed Uberlandia, Inst Biomed Sci, Lab Immunoparasitol Dr Mario Endsfeldz Camargo, Uberlandia, MG, Brazil.
[Davoli-Ferreira, Marcela; Fonseca, Denise M.; Dias, Murilo S.; Lima-Junior, Djalma S.; Quirino, Gustavo F. S.; Silva, Joao S.] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Biochem & Immunol, Ribeirao Preto, SP, Brazil.
[Fonseca, Denise M.] NIAID, Program Barrier Immun & Repair Mucosal Immunol Se, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA.
[Zamboni, Dario S.] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Cell Biol, Ribeirao Preto, SP, Brazil.
RP Mineo, TWP (reprint author), Univ Fed Uberlandia, Inst Biomed Sci, Lab Immunoparasitol Dr Mario Endsfeldz Camargo, Uberlandia, MG, Brazil.
EM tiago.mineo@ufu.br
RI Mineo, Tiago/B-4153-2009; Silva, Joao/A-4484-2008; CEPID,
CRID/J-2644-2015;
OI Mineo, Tiago/0000-0002-2339-2743; Souza Lima Junior,
Djalma/0000-0002-2092-8047; Zamboni, Dario/0000-0002-7856-7512
NR 40
TC 1
Z9 1
U1 0
U2 2
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2045-2322
J9 SCI REP-UK
JI Sci Rep
PD JUL 5
PY 2016
VL 6
AR 29289
DI 10.1038/srep29289
PG 14
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ6AE
UT WOS:000379285600001
PM 27377650
ER
PT J
AU Smith, RH
Hallwirth, CV
Westerman, M
Hetherington, NA
Tseng, YS
Cecchini, S
Virag, T
Ziegler, ML
Rogozin, IB
Koonin, EV
Agbandje-McKenna, M
Kotin, RM
Alexander, IE
AF Smith, Richard H.
Hallwirth, Claus V.
Westerman, Michael
Hetherington, Nicola A.
Tseng, Yu-Shan
Cecchini, Sylvain
Virag, Tamas
Ziegler, Mona-Larissa
Rogozin, Igor B.
Koonin, Eugene V.
Agbandje-McKenna, Mavis
Kotin, Robert M.
Alexander, Ian E.
TI Germline viral "fossils" guide in silico Ereconstruction of a
mid-Cenozoic era marsupial adeno-associated virus
SO SCIENTIFIC REPORTS
LA English
DT Article
ID MULTIPLE SEQUENCE ALIGNMENT; HUMAN GENE-THERAPY; DNA-REPLICATION;
PHYLOGENETIC INFERENCE; TRANSCRIPTION FACTOR; MAMMALIAN GENOMES;
CRYSTAL-STRUCTURE; BINDING-SITE; EVOLUTION; PROTEIN
AB Germline endogenous viral elements (EVEs) genetically preserve viral nucleotide sequences useful to the study of viral evolution, gene mutation, and the phylogenetic relationships among host organisms. Here, we describe a lineage-specific, adeno-associated virus (AAV)-derived endogenous viral element (mAAV-EVE1) found within the germline of numerous closely related marsupial species. Molecular screening of a marsupial DNA panel indicated that mAAV-EVE1 occurs specifically within the marsupial suborder Macropodiformes (present-day kangaroos, wallabies, and related macropodoids), to the exclusion of other Diprotodontian lineages. Orthologous mAAV-EVE1 locus sequences from sixteen macropodoid species, representing a speciation history spanning an estimated 30 million years, facilitated compilation of an inferred ancestral sequence that recapitulates the genome of an ancient marsupial AAV that circulated among Australian metatherian fauna sometime during the late Eocene to early Oligocene. In silico gene reconstruction and molecular modelling indicate remarkable conservation of viral structure over a geologic timescale. Characterisation of AAV-EVE loci among disparate species affords insight into AAV evolution and, in the case of macropodoid species, may offer an additional genetic basis for assignment of phylogenetic relationships among the Macropodoidea. From an applied perspective, the identified AAV "fossils" provide novel capsid sequences for use in translational research and clinical applications.
C1 [Smith, Richard H.; Cecchini, Sylvain; Virag, Tamas; Kotin, Robert M.] NHLBI, Lab Mol Virol & Gene Therapy, Bldg 10, Bethesda, MD 20892 USA.
[Hallwirth, Claus V.; Hetherington, Nicola A.; Ziegler, Mona-Larissa; Alexander, Ian E.] Childrens Med Res Inst, Gene Therapy Res Unit, Westmead, NSW, Australia.
[Hallwirth, Claus V.; Hetherington, Nicola A.; Ziegler, Mona-Larissa; Alexander, Ian E.] Childrens Hosp Westmead, Westmead, NSW, Australia.
[Westerman, Michael] La Trobe Univ, Dept Biochem & Genet, Bundoora, Vic, Australia.
[Tseng, Yu-Shan; Agbandje-McKenna, Mavis] Univ Florida, McKnight Brain Inst, Gainesville, FL USA.
[Rogozin, Igor B.; Koonin, Eugene V.] NIH, Evolutionary Genom Res Grp, Natl Ctr Biotechnol Informat, Natl Lib Med, Bldg 10, Bethesda, MD 20892 USA.
[Alexander, Ian E.] Univ Sydney, Discipline Paediat & Child Hlth, Westmead, NSW, Australia.
[Kotin, Robert M.] Voyager Therapeut, Cambridge, MA USA.
[Kotin, Robert M.] Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA USA.
RP Kotin, RM (reprint author), NHLBI, Lab Mol Virol & Gene Therapy, Bldg 10, Bethesda, MD 20892 USA.; Alexander, IE (reprint author), Childrens Med Res Inst, Gene Therapy Res Unit, Westmead, NSW, Australia.; Alexander, IE (reprint author), Childrens Hosp Westmead, Westmead, NSW, Australia.; Alexander, IE (reprint author), Univ Sydney, Discipline Paediat & Child Hlth, Westmead, NSW, Australia.; Kotin, RM (reprint author), Voyager Therapeut, Cambridge, MA USA.; Kotin, RM (reprint author), Univ Massachusetts, Sch Med, Gene Therapy Ctr, Worcester, MA USA.
EM robert_kotin@umassmed.edu; ian.alexander@health.nsw.gov.au
FU Intramural Research Program of the National Library of Medicine
(National Institutes of Health, US Department of Health and Human
Services); Australian Research Council [DP150101253]; NIH [R01
GM082946]; Intramural Research Program of the National Heart, Lung, and
Blood Institute, of the National Institutes of Health
FX We thank the San Diego Zoo Global and Mark Springer (University of
California, Riverside, California, USA) for providing marsupial DNA
samples. IBR and EVK were supported by the Intramural Research Program
of the National Library of Medicine (National Institutes of Health, US
Department of Health and Human Services). CVH and NAH are supported by a
Discovery Project grant (DP150101253) from the Australian Research
Council. MA-M was supported by NIH R01 GM082946. RHS, SC, TV, and RMK
were supported by the Intramural Research Program of the National Heart,
Lung, and Blood Institute, of the National Institutes of Health.
NR 95
TC 1
Z9 1
U1 2
U2 2
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2045-2322
J9 SCI REP-UK
JI Sci Rep
PD JUL 5
PY 2016
VL 6
AR 28965
DI 10.1038/srep28965
PG 17
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DQ4QO
UT WOS:000379188600001
PM 27377618
ER
PT J
AU Carson, SS
Cox, CE
Wallenstein, S
Hanson, LC
Danis, M
Tulsky, JA
Chai, E
Nelson, JE
AF Carson, Shannon S.
Cox, Christopher E.
Wallenstein, Sylvan
Hanson, Laura C.
Danis, Marion
Tulsky, James A.
Chai, Emily
Nelson, Judith E.
TI Effect of Palliative Care-Led Meetings for Families of Patients With
Chronic Critical Illness A Randomized Clinical Trial
SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
LA English
DT Article
ID PROLONGED MECHANICAL VENTILATION; OF-LIFE CARE; COMMUNICATION-SKILLS;
RESOURCE UTILIZATION; DEPRESSION SCALE; HOSPITAL ANXIETY; UNITED-STATES;
ILL PATIENTS; ICU; VALIDATION
AB IMPORTANCE Family caregivers of patients with chronic critical illness experience significant psychological distress.
OBJECTIVE To determine whether family informational and emotional support meetings led by palliative care clinicians improve family anxiety and depression.
DESIGN, SETTING, AND PARTICIPANTS A multicenter randomized clinical trial conducted from October 2010 through November 2014 in 4 medical intensive care units (ICUs). Adult patients (aged >= 21 years) requiring 7 days of mechanical ventilation were randomized and their family surrogate decision makers were enrolled in the study. Observers were blinded to group allocation for the measurement of the primary outcomes.
INTERVENTIONS At least 2 structured family meetings led by palliative care specialists and provision of an informational brochure (intervention) compared with provision of an informational brochure and routine family meetings conducted by ICU teams (control). There were 130 patients with 184 family surrogate decision makers in the intervention group and 126 patients with 181 family surrogate decision makers in the control group.
MAIN OUTCOMES AND MEASURES The primary outcome was Hospital Anxiety and Depression Scale symptom score (HADS; score range, 0[best] to 42[worst]; minimal clinically important difference, 1.5) obtained during 3-month follow-up interviews with the surrogate decision makers. Secondary outcomes included posttraumatic stress disorder experienced by the family and measured by the Impact of Events Scale-Revised (IES-R; total score range, 0 [best] to 88 [worst]), discussion of patient preferences, hospital length of stay, and 90-day survival.
RESULTS Among 365 family surrogate decision makers (mean age, 51 years; 71% female), 312 completed the study. At 3 months, there was no significant difference in anxiety and depression symptoms between surrogate decision makers in the intervention group and the control group (adjusted mean HADS score, 12.2 vs 11.4, respectively; between-group difference, 0.8 [95% CI, -0.9 to 2.6]; P = .34). Posttraumatic stress disorder symptoms were higher in the intervention group (adjusted mean IES-R score, 25.9) compared with the control group (adjusted mean IES-R score, 21.3) (between-group difference, 4.60 [95% CI, 0.01 to 9.10]; P = .0495). There was no difference between groups regarding the discussion of patient preferences (intervention, 75%; control, 83%; odds ratio, 0.63[95% CI, 0.34 to 1.16; P = .14]). The median number of hospital days for patients in the intervention vs the control group (19 days vs 23 days, respectively; between-group difference, -4 days [95% CI, -6 to 3 days]; P = .51) and 90-day survival (hazard ratio, 0.95 [95% CI, 0.65 to 1.38], P = .96) were not significantly different.
CONCLUSIONS AND RELEVANCE Among families of patients with chronic critical illness, the use of palliative care-led informational and emotional support meetings compared with usual care did not reduce anxiety or depression symptoms and may have increased posttraumatic stress disorder symptoms. These findings do not support routine or mandatory palliative care-led discussion of goals of care for all families of patients with chronic critical illness.
C1 [Carson, Shannon S.; Hanson, Laura C.] Univ N Carolina, Sch Med, Chapel Hill, NC USA.
[Cox, Christopher E.] Duke Univ, Med Ctr, Durham, NC USA.
[Wallenstein, Sylvan; Chai, Emily] Icahn Sch Med Mt Sinai, New York, NY 10029 USA.
[Danis, Marion] NIH, Bldg 10, Bethesda, MD 20892 USA.
[Tulsky, James A.] Harvard Univ, Sch Med, Dana Farber Canc Inst, 44 Binney St, Boston, MA 02115 USA.
[Tulsky, James A.] Harvard Univ, Sch Med, Brigham & Womens Hosp, Boston, MA USA.
[Nelson, Judith E.] Mem Sloan Kettering Canc Ctr, 1275 York Ave, New York, NY 10021 USA.
[Nelson, Judith E.] Weill Cornell Med Coll, New York, NY USA.
RP Carson, SS (reprint author), Univ N Carolina, Sch Med, Div Pulm & Crit Care Med, Marsico Lung Inst,Cecil B Sheps Ctr Hlth Serv Res, 4125 Bioinformat Bldg,CB 7020, Chapel Hill, NC 27599 USA.
EM scarson@med.unc.edu
FU National Institute of Nursing Research [R01-NR012413]
FX This project was funded by grant R01-NR012413 from the National
Institute of Nursing Research.
NR 36
TC 15
Z9 16
U1 13
U2 15
PU AMER MEDICAL ASSOC
PI CHICAGO
PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA
SN 0098-7484
EI 1538-3598
J9 JAMA-J AM MED ASSOC
JI JAMA-J. Am. Med. Assoc.
PD JUL 5
PY 2016
VL 316
IS 1
BP 51
EP 62
DI 10.1001/jama.2016.8474
PG 12
WC Medicine, General & Internal
SC General & Internal Medicine
GA DQ2MY
UT WOS:000379037600018
PM 27380343
ER
PT J
AU Katz, JN
Minder, M
Olenchock, B
Price, S
Goldfarb, M
Washam, JB
Barnett, CF
Newby, LK
van Diepen, S
AF Katz, Jason N.
Minder, Michael
Olenchock, Benjamin
Price, Susanna
Goldfarb, Michael
Washam, Jeffrey B.
Barnett, Christopher F.
Newby, L. Kristin
van Diepen, Sean
TI The Genesis, Maturation, and Future of Critical Care Cardiology
SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
LA English
DT Review
DE quality of care; research; training
ID ELEVATION MYOCARDIAL-INFARCTION; INTENSIVE-CARE; ILL PATIENTS;
UNITED-STATES; NURSE-PRACTITIONERS; AMERICAN-COLLEGE; TEMPORAL TRENDS;
CARDIAC-ARREST; SEPTIC SHOCK; TASK-FORCE
AB The cardiac intensive care unit (CICU) has changed considerably over time and now serves a unique patient population with a high burden of cardiovascular and noncardiovascular critical illness. Patient complexity and technological evolutions in the CICU have catalyzed the development of critical care cardiology, a fledgling discipline that combines specialization in cardiovascular diseases with knowledge and experience in critical care medicine. Numerous uncertainties and challenges threaten to stymie the growth of this field. A multidisciplinary dialogue focused on the best care design for the CICU patient is needed as we consider alternative approaches to clinical training, staffing, and investigation in this rapidly evolving arena. (J Am Coll Cardiol 2016; 68:67-79) (C) 2016 by the American College of Cardiology Foundation.
C1 [Katz, Jason N.] Univ N Carolina, Sch Med, Div Cardiol, Chapel Hill, NC USA.
[Katz, Jason N.] Univ N Carolina, Sch Med, Div Pulm & Crit Care Med, Chapel Hill, NC USA.
[Minder, Michael; Washam, Jeffrey B.; Newby, L. Kristin] Duke Univ, Div Cardiol, Durham, NC USA.
[Olenchock, Benjamin] Brigham & Womens Hosp, Div Cardiovasc Med, 75 Francis St, Boston, MA 02115 USA.
[Price, Susanna] Royal Brompton Hosp, Div Cardiol, London SW3 6LY, England.
[Price, Susanna] Royal Brompton Hosp, Div Crit Care, London SW3 6LY, England.
[Goldfarb, Michael] McGill Univ, Div Cardiol, Montreal, PQ, Canada.
[Barnett, Christopher F.] Univ Calif San Francisco, Div Cardiol, San Francisco, CA USA.
[Barnett, Christopher F.; Newby, L. Kristin] NIH, Dept Crit Care Med, Bethesda, MD 20892 USA.
[Newby, L. Kristin] Duke Clin Res Inst, Durham, NC USA.
[van Diepen, Sean] Univ Alberta Hosp, Div Cardiol, Edmonton, AB, Canada.
[van Diepen, Sean] Univ Alberta Hosp, Div Crit Care, Edmonton, AB, Canada.
RP Katz, JN (reprint author), UNC Ctr Heart & Vasc Care, 160 Dent Circle,CB 7075, Chapel Hill, NC 27599 USA.
EM katzj@med.unc.edu
FU Heart and Stroke Foundation; University Hospital Foundation; National
Institutes of Health; Patient-Centered Outcomes Research Institute
FX Dr. van Diepen has received grant support from the Heart and Stroke
Foundation and the University Hospital Foundation. Dr. Newby is a
consultant for BioKier, Metanomics, Merck, Roche Diagnostics, and
Philips Healthcare; is a researcher for Metanomics, Sanofi,
GlaxoSmithKline, and Verily (formerly Google Life Sciences); is on the
data and safety monitoring board of DemeRx; is on the advisory board of
MedScape/theHeart.org; has received research funding from the National
Institutes of Health and the Patient-Centered Outcomes Research
Institute; and has received honoraria from JACC: Basic to Translational
Science and the Journal of the American Heart Association. All other
authors have reported that they have no relationships relevant to the
contents of this paper to disclose.
NR 79
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U1 3
U2 4
PU ELSEVIER SCIENCE INC
PI NEW YORK
PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
SN 0735-1097
EI 1558-3597
J9 J AM COLL CARDIOL
JI J. Am. Coll. Cardiol.
PD JUL 5
PY 2016
VL 68
IS 1
BP 67
EP 79
DI 10.1016/j.jacc.2016.04.036
PG 13
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA DP5IB
UT WOS:000378528400011
PM 27364053
ER
PT J
AU Sakamuri, SSVP
Valente, AJ
Siddesha, JM
Delafontaine, P
Siebenlist, U
Gardner, JD
Bysani, C
AF Sakamuri, Siva S. V. P.
Valente, Anthony J.
Siddesha, Jalahalli M.
Delafontaine, Patrice
Siebenlist, Ulrich
Gardner, Jason D.
Bysani, Chandrasekar
TI TRAF3IP2 mediates aldosterone/salt-induced cardiac hypertrophy and
fibrosis
SO MOLECULAR AND CELLULAR ENDOCRINOLOGY
LA English
DT Article
DE RAAS; Aldosterone; TRAF3IP2; CIKS; Act1; Cardiac fibrosis; Cardiac
hypertrophy
ID LEFT-VENTRICULAR HYPERTROPHY; ACTIVATED PROTEIN-KINASE; ANGIOTENSIN-II;
HEART-FAILURE; DIASTOLIC DYSFUNCTION; MYOCARDIAL-INFARCTION; FIBROBLAST
MIGRATION; OXIDATIVE STRESS; RECEPTOR; EXPRESSION
AB Aberrant activation of the renin-angiotensin-aldosterone system (RAAS) contributes to adverse cardiac remodeling and eventual failure. Here we investigated whether TRAF3 Interacting Protein 2 (TRAF3IP2), a redox-sensitive cytoplasmic adaptor molecule and an upstream regulator of nuclear factor-kappa B (NF-kappa B) and activator protein-1 (AP-1), mediates aldosterone-induced cardiac hypertrophy and fibrosis. Wild type (WT) and TRAF3IP2-null mice were infused with aldosterone (0.2 mg/kg/day) for 4 weeks along with 1%NaCl in drinking water. Aldosterone/salt, but not salt alone, upregulated TRAF3IP2 expression in WT mouse hearts. Further, aldosterone elevated blood pressure to a similar extent in both WT and TRAF3IP2-null groups. However, TRAF3IP2 gene deletion attenuated aldosterone/salt-induced (i) p65 and c-Jun activation, (ii) extracellular matrix (collagen I alpha 1 and collagen III alpha 1), matrix metalloproteinase (MMP2), lysyl oxidase (LOX), inflammatory cytokine (IL-6 and IL-18), chemokine (CXCL1 and CXCL2), and adhesion molecule (ICAM1) mRNA expression in hearts, (iii) IL-6, IL-18, and MMP2 protein levels, (iv) systemic IL-6 and IL-18 levels, and (iv) cardiac hypertrophy and fibrosis. These results indicate that TRAF3IP2 is a critical signaling intermediate in aldosterone/salt-induced myocardial hypertrophy and fibrosis, and thus a potential therapeutic target in hypertensive heart disease. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
C1 [Sakamuri, Siva S. V. P.; Siddesha, Jalahalli M.; Delafontaine, Patrice; Bysani, Chandrasekar] Tulane Univ, Sch Med, Inst Heart & Vasc, New Orleans, LA 70112 USA.
[Valente, Anthony J.] Univ Texas Hlth Sci Ctr San Antonio, Med, San Antonio, TX 78229 USA.
[Siebenlist, Ulrich] NIAID, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA.
[Gardner, Jason D.] Louisiana State Univ, Hlth Sci Ctr, Physiol, New Orleans, LA 70112 USA.
[Sakamuri, Siva S. V. P.] Univ Alberta, Dept Physiol, Edmonton, AB T6G 2S2, Canada.
[Siddesha, Jalahalli M.] Univ Vermont, Lab Pathol & Med, Burlington, VT 05405 USA.
[Delafontaine, Patrice; Bysani, Chandrasekar] Univ Missouri, Sch Med, Dept Med, Columbia, MO 65212 USA.
[Bysani, Chandrasekar] Harry S Truman Mem Vet Hosp, Res Serv, Columbia, MO 65212 USA.
RP Bysani, C (reprint author), Harry S Truman Mem Vet Hosp, Res Serv, 800 Hosp Dr, Columbia, MO 65201 USA.
EM chandrasekarb@health.missouri.edu
OI Delafontaine, Patrice/0000-0003-3744-3617
FU Department of Veterans Affairs; VA Office of Research and Development
Biomedical Laboratory Research and Development Service [I01-BX002255];
NIH/NHLBI [HL-86787]; NHLBI [HL-70241, HL-80682]; NIH/NIAID
FX BC is a recipient of the Department of Veterans Affairs Research Career
Scientist award and is supported by VA Office of Research and
Development Biomedical Laboratory Research and Development Service Award
I01-BX002255 and the NIH/NHLBI grant HL-86787. PD is supported by NHLBI
grants HL-70241 and HL-80682. US is supported by the Intramural Research
Program of the NIH/NIAID. The contents of this report do not represent
the views of the Department of Veterans Affairs or the United States
government.
NR 40
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U1 0
U2 4
PU ELSEVIER IRELAND LTD
PI CLARE
PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000,
IRELAND
SN 0303-7207
J9 MOL CELL ENDOCRINOL
JI Mol. Cell. Endocrinol.
PD JUL 5
PY 2016
VL 429
IS C
BP 84
EP 92
DI 10.1016/j.mce.2016.03.038
PG 9
WC Cell Biology; Endocrinology & Metabolism
SC Cell Biology; Endocrinology & Metabolism
GA DN1CI
UT WOS:000376803500009
PM 27040306
ER
PT J
AU Zhou, B
Yu, PP
Lin, MY
Sun, T
Chen, YM
Sheng, ZH
AF Zhou, Bing
Yu, Panpan
Lin, Mei-Yao
Sun, Tao
Chen, Yanmin
Sheng, Zu-Hang
TI Facilitation of axon regeneration by enhancing mitochondrial transport
and rescuing energy deficits
SO JOURNAL OF CELL BIOLOGY
LA English
DT Article
ID RETINAL GANGLION-CELLS; SPINAL-CORD-INJURY; WALLERIAN DEGENERATION;
NERVE REGENERATION; CORTICAL-NEURONS; GROWTH; GUIDANCE; SURVIVAL;
PROTEIN; WLD(S)
AB Although neuronal regeneration is a highly energy-demanding process, axonal mitochondria! transport progressively declines with maturation. Mature neurons typically fail to regenerate after injury, thus raising a fundamental question as to whether mitochondrial transport is necessary to meet enhanced metabolic requirements during regeneration. Here, we reveal that reduced mitochondrial motility and energy deficits in injured axons are intrinsic mechanisms controlling regrowth in mature neurons. Axotomy induces acute mitochondria! depolarization and ATP depletion in injured axons. Thus, mature neuron-associated increases in mitochondria-anchoring protein syntaphilin (SNPH) and decreases in mitochondrial transport cause local energy deficits. Strikingly, enhancing mitochondria! transport via genetic manipulation facilitates regenerative capacity by replenishing healthy mitochondria in injured axons, thereby rescuing energy deficits. An in vivo sciatic nerve crush study further shows that enhanced mitochondrial transport in snph knockout mice accelerates axon regeneration. Understanding deficits in mitochondria! trafficking and energy supply in injured axons of mature neurons benefits development of new strategies to stimulate axon regeneration.
C1 [Zhou, Bing; Lin, Mei-Yao; Sun, Tao; Chen, Yanmin; Sheng, Zu-Hang] NINDS, Synapt Funct Sect, Porter Neurosci Res Ctr, NIH, Bethesda, MD 20892 USA.
[Yu, Panpan] Jinan Univ, Guangdong Hong Kong Macau Inst CNS Regenerat, Joint Int Res Lab CNS Regenerat, Minist Educ, Guangzhou 510632, Guangdong, Peoples R China.
RP Sheng, ZH (reprint author), NINDS, Synapt Funct Sect, Porter Neurosci Res Ctr, NIH, Bethesda, MD 20892 USA.
EM shengz@ninds.nih.gov
FU NIH [ZIA NS003029, ZIA NS002946]; Intramural Research Program of NINDS
FX The work was supported by the Intramural Research Program of NINDS and
NIH ZIA NS003029 and ZIA NS002946 (to Z.-H. Sheng).
NR 65
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Z9 8
U1 10
U2 14
PU ROCKEFELLER UNIV PRESS
PI NEW YORK
PA 950 THIRD AVE, 2ND FLR, NEW YORK, NY 10022 USA
SN 0021-9525
EI 1540-8140
J9 J CELL BIOL
JI J. Cell Biol.
PD JUL 4
PY 2016
VL 214
IS 1
BP 103
EP 119
DI 10.1083/jcb.201605101
PG 17
WC Cell Biology
SC Cell Biology
GA DQ5ZJ
UT WOS:000379283500012
PM 27268498
ER
PT J
AU Shao, W
Shan, JG
Kearney, MF
Wu, XL
Maldarelli, F
Mellors, JW
Luke, B
Coffin, JM
Hughes, SH
AF Shao, Wei
Shan, Jigui
Kearney, Mary F.
Wu, Xiaolin
Maldarelli, Frank
Mellors, John W.
Luke, Brian
Coffin, John M.
Hughes, Stephen H.
TI Retrovirus Integration Database (RID): a public database for retroviral
insertion sites into host genomes
SO RETROVIROLOGY
LA English
DT Article
DE Retrovirus; HIV; Integration site; Database; Integration site assay;
ISA; Expanded clones
ID HIV-1 INTEGRATION; GENES; CELLS; DNA; VIRUS; ENHANCERS; SELECTION;
SEQUENCE; THERAPY; TARGETS
AB The NCI Retrovirus Integration Database is a MySql-based relational database created for storing and retrieving comprehensive information about retroviral integration sites, primarily, but not exclusively, HIV-1. The database is accessible to the public for submission or extraction of data originating from experiments aimed at collecting information related to retroviral integration sites including: the site of integration into the host genome, the virus family and subtype, the origin of the sample, gene exons/introns associated with integration, and proviral orientation. Information about the references from which the data were collected is also stored in the database. Tools are built into the website that can be used to map the integration sites to UCSC genome browser, to plot the integration site patterns on a chromosome, and to display provirus LTRs in their inserted genome sequence. The website is robust, user friendly, and allows users to query the database and analyze the data dynamically.
C1 [Shao, Wei; Shan, Jigui; Luke, Brian] FNLCR, Leidos Biomed Res Inc, Adv Biomed Comp Ctr, Frederick, MD USA.
[Kearney, Mary F.; Maldarelli, Frank; Hughes, Stephen H.] NCI, HIV Dynam & Replicat Program, Frederick, MD 21701 USA.
[Wu, Xiaolin] Frederick Natl Lab Canc Res, Leidos Biomed Res Inc, Frederick, MD USA.
[Mellors, John W.] Univ Pittsburgh, Div Infect Dis, Pittsburgh, PA USA.
[Coffin, John M.] Tufts Univ, Dept Mol Biol & Microbiol, Boston, MA 02111 USA.
RP Shao, W (reprint author), FNLCR, Leidos Biomed Res Inc, Adv Biomed Comp Ctr, Frederick, MD USA.
EM shaow@mail.nih.gov
FU NCI CCR; Office of AIDS Research, NIH; NCI [HHSN261200800001E]
FX We acknowledge the funding sources for this study from NCI CCR, the
Office of AIDS Research, NIH, and NCI Contract No. HHSN261200800001E.
NR 20
TC 1
Z9 1
U1 3
U2 3
PU BIOMED CENTRAL LTD
PI LONDON
PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
SN 1742-4690
J9 RETROVIROLOGY
JI Retrovirology
PD JUL 4
PY 2016
VL 13
AR 47
DI 10.1186/s12977-016-0277-6
PG 9
WC Virology
SC Virology
GA DQ5TL
UT WOS:000379267800001
PM 27377064
ER
PT J
AU Louis, JM
Roche, J
AF Louis, John M.
Roche, Julien
TI Evolution under Drug Pressure Remodels the Folding Free-Energy Landscape
of Mature HIV-1 Protease
SO JOURNAL OF MOLECULAR BIOLOGY
LA English
DT Article
DE HIV protease; drug resistance; protein folding landscape; high-pressure
NMR; calorimetry
ID CLINICAL INHIBITORS; RESISTANT MUTANT; NMR-SPECTROSCOPY; MUTATIONS;
DYNAMICS; SIMULATIONS; STABILITY; VARIANTS; PROTEINS; MONOMER
AB Using high-pressure NMR spectroscopy and differential scanning calorimetry, we investigate the folding landscape of the mature HIV-1 protease homodimer. The cooperativity of unfolding was measured in the absence or presence of a symmetric active site inhibitor for the optimized wild type protease (PR), its inactive variant PRD25N, and an extremely multidrug-resistant mutant, PR20. The individual fit of the pressure denaturation profiles gives rise to first order, Delta G(NMR), and second order, Delta V-NMR (the derivative of Delta G(NMR) with pressure); apparent thermodynamic parameters for each amide proton considered. Heterogeneity in the apparent Delta V-NMR values reflects departure from an ideal cooperative unfolding transition. The narrow to broad distribution of Delta V-NMR spanning the extremes from inhibitor-free PR20(D25N) to PR-DMP323 complex, and distinctively for PRD25N-DMP323 complex, indicated large variations in folding cooperativity. Consistent with this data, the shape of thermal unfolding transitions varies from asymmetric for PR to nearly symmetric for PR20, as dimer-inhibitor ternary complexes. Lack of structural cooperativity was observed between regions located close to the active site, including the hinge and tip of the glycine-rich flaps, and the rest of the protein. These results strongly suggest that inhibitor binding drastically decreases the cooperativity of unfolding by trapping the closed flap conformation in a deep energy minimum. To evade this conformational trap, PR20 evolves exhibiting a smoother folding landscape with nearly an ideal two-state (cooperative) unfolding transition. This study highlights the malleability of retroviral protease folding pathways by illustrating how the selection of mutations under drug pressure remodels the free-energy landscape as a primary mechanism. Published by Elsevier Ltd.
C1 [Louis, John M.; Roche, Julien] NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
[Roche, Julien] Iowa State Univ, Roy J Carver Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA.
RP Louis, JM; Roche, J (reprint author), NIDDK, LCP, NIH, Bldg 5,Room B1-27, Bethesda, MD 20892 USA.
EM johnl@niddk.nih.gov; julien.roche@nih.gov
FU Intramural Research Program of the NIDDK, National Institutes of Health;
Intramural AIDS-Targeted Program of the Office of the Director, NIH
FX We thank Hoi Sung Chung and Jane M. Sayer for discussions, Annie Aniana
for technical assistance, and Ad Bax for critical review of the work and
support. This research was supported by the Intramural Research Program
of the NIDDK, National Institutes of Health, and the Intramural
AIDS-Targeted Program of the Office of the Director, NIH. Clinical
protease inhibitor daruvanir was obtained through the NIH AIDS Research
and Reference Reagent Program, Division of AIDS, NIAID, NIH. We
acknowledge the use of the NIDDK Advanced Mass Spectrometry Core
Facility.
NR 48
TC 2
Z9 2
U1 5
U2 21
PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
PI LONDON
PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND
SN 0022-2836
EI 1089-8638
J9 J MOL BIOL
JI J. Mol. Biol.
PD JUL 3
PY 2016
VL 428
IS 13
BP 2780
EP 2792
DI 10.1016/j.jmb.2016.05.005
PG 13
WC Biochemistry & Molecular Biology
SC Biochemistry & Molecular Biology
GA DQ1JY
UT WOS:000378958500009
PM 27170547
ER
PT J
AU Farmer, C
Golden, C
Thurm, A
AF Farmer, Cristan
Golden, Christine
Thurm, Audrey
TI Concurrent validity of the differential ability scales, second edition
with the Mullen Scales of Early Learning in young children with and
without neurodevelopmental disorders
SO CHILD NEUROPSYCHOLOGY
LA English
DT Article
DE IQ; Mullen Scales of Early Learning; Cognitive testing; DAS-II;
Developmental quotient; Autism
ID AUTISM SPECTRUM DISORDERS; INTELLECTUAL DISABILITIES; WISC-IV;
INTELLIGENCE; PRESCHOOL; LEVEL; LIMITATIONS; DIAGNOSIS; STABILITY
AB Estimates of intelligence in young children with neurodevelopmental disorders are critical for making diagnoses, in characterizing symptoms of disorders, and in predicting future outcomes. The limitations of standardized testing for children with developmental delay or cognitive impairment are well known: Tests do not exist that provide developmentally appropriate material along with norms that extend to the lower reaches of ability. Two commonly used and interchanged instruments are the Mullen Scales of Early Learning (MSEL), a test of developmental level, and the Differential Ability Scales, second edition (DAS-II), a more traditional cognitive test. We evaluated the correspondence of contemporaneous MSEL and the DAS-II scores in a mixed sample of children aged 2-10years with autism spectrum disorder (ASD), non-ASD developmental delays, and typically developing children across the full spectrum of cognitive ability. Consistent with published data on the original DAS and the MSEL, scores on the DAS-II and MSEL were highly correlated. However, curve estimation revealed large mean differences that varied as a function of the child's cognitive ability level. We conclude that interchanging MSEL and DAS-II scores without regard to the discrepancy in scores may produce misleading results in both cross-sectional and longitudinal studies of children with and without ASD, and, thus, this practice should be implemented with caution.
C1 [Farmer, Cristan; Golden, Christine; Thurm, Audrey] NIH, Pediat & Dev Neurosci Branch, Bldg 10, Bethesda, MD 20892 USA.
RP Thurm, A (reprint author), NIMH, Pediat & Dev Neurosci Branch, 10 Ctr Dr MSC 1255,Bldg 10,Room 1C250, Bethesda, MD 20892 USA.
EM athurm@mail.nih.gov
FU Intramural Program of National Institute of Mental Health of National
Institutes of Health [NCT00298246, 06-M-0102]
FX This work was supported by the Intramural Program of the National
Institute of Mental Health of the National Institutes of Health,
NCT00298246, 06-M-0102. The views expressed in this paper do not
necessarily represent the views of the NIMH, NIH, HHS, or the United
States Government. The authors extend their gratitude to the children
and their families who volunteered their time and efforts during the
research.
NR 34
TC 2
Z9 2
U1 6
U2 19
PU ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD
PI ABINGDON
PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXFORDSHIRE, ENGLAND
SN 0929-7049
EI 1744-4136
J9 CHILD NEUROPSYCHOL
JI Child Neuropsychol.
PD JUL 3
PY 2016
VL 22
IS 5
BP 556
EP 569
DI 10.1080/09297049.2015.1020775
PG 14
WC Clinical Neurology
SC Neurosciences & Neurology
GA DJ0FC
UT WOS:000373876200003
PM 25833070
ER
PT J
AU Solomon, BD
Lee, T
Nguyen, AD
Wolfsberg, TG
AF Solomon, Benjamin D.
Lee, Teresa
Anh-Dao Nguyen
Wolfsberg, Tyra G.
TI A 2.5-year snapshot of Mendelian discovery
SO MOLECULAR GENETICS & GENOMIC MEDICINE
LA English
DT Editorial Material
ID CLINICAL GENOME; DISORDERS; DISEASE
C1 [Solomon, Benjamin D.] Inova Hlth Syst, Inova Translat Med Inst, Div Med Genom, Falls Church, VA 22042 USA.
[Solomon, Benjamin D.; Lee, Teresa] Inova Hlth Syst, Inova Childrens Hosp, Dept Pediat, Falls Church, VA 22042 USA.
[Solomon, Benjamin D.] Virginia Commonwealth Univ, Sch Med, Dept Pediat, Richmond, VA 23298 USA.
[Anh-Dao Nguyen; Wolfsberg, Tyra G.] Natl Human Genome Res Inst, Computat & Stat Genom Branch, Bethesda, MD 20892 USA.
RP Solomon, BD (reprint author), Inova Hlth Syst, Inova Translat Med Inst, Div Med Genom, Falls Church, VA 22042 USA.; Solomon, BD (reprint author), Inova Hlth Syst, Inova Childrens Hosp, Dept Pediat, Falls Church, VA 22042 USA.; Solomon, BD (reprint author), Virginia Commonwealth Univ, Sch Med, Dept Pediat, Richmond, VA 23298 USA.
FU Intramural Research Program of the National Human Genome Research
Institute, National Institutes of Health
FX This research was supported in part by the Intramural Research Program
of the National Human Genome Research Institute, the National Institutes
of Health.
NR 12
TC 0
Z9 0
U1 0
U2 0
PU WILEY
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2324-9269
J9 MOL GENET GENOM MED
JI Mol. Genet. Genom. Med.
PD JUL
PY 2016
VL 4
IS 4
BP 392
EP 394
DI 10.1002/mgg3.221
PG 3
WC Genetics & Heredity
SC Genetics & Heredity
GA EL2KX
UT WOS:000394449700003
PM 27468415
ER
PT J
AU Tobe, R
Carlson, BA
Huh, JH
Castro, NP
Xu, XM
Tsuji, PA
Lee, SG
Bang, J
Na, JW
Kong, YY
Beaglehole, D
Southon, E
Seifried, H
Tessarollo, L
Salomon, DS
Schweizer, U
Gladyshev, VN
Hatfield, DL
Lee, BJ
AF Tobe, Ryuta
Carlson, Bradley A.
Huh, Jang Hoe
Castro, Nadia P.
Xu, Xue-Ming
Tsuji, Petra A.
Lee, Sang-Goo
Bang, Jeyoung
Na, Ji-Woon
Kong, Young-Yun
Beaglehole, Daniel
Southon, Eileen
Seifried, Harold
Tessarollo, Lino
Salomon, David S.
Schweizer, Ulrich
Gladyshev, Vadim N.
Hatfield, Dolph L.
Lee, Byeong Jae
TI Selenophosphate synthetase 1 is an essential protein with roles in
regulation of redox homoeostasis in mammals
SO BIOCHEMICAL JOURNAL
LA English
DT Article
DE cancer; reactive oxygen species (ROS); redox regulation; selenium;
selenocysteine; selenophosphate synthetase 1
ID SELENOPROTEIN BIOSYNTHESIS; GLUTATHIONE TRANSFERASE; THIOREDOXIN
REDUCTASE; GENETIC-CODE; SELENOCYSTEINE; SELENIUM; METABOLISM;
EXPRESSION; DROSOPHILA; CELLS
AB Selenophosphate synthetase (SPS) was initially detected in bacteria and was shown to synthesize selenophosphate, the active selenium donor. However, mammals have two SPS paralogues, which are designated SPS1 and SPS2. Although it is known that SPS2 catalyses the synthesis of selenophosphate, the function of SPS1 remains largely unclear. To examine the role of SPS1 in mammals, we generated a Sps1-knockout mouse and found that systemic SPS1 deficiency led to embryos that were clearly underdeveloped by embryonic day (E)8.5 and virtually resorbed by E14.5. The knockout of Sps1 in the liver preserved viability, but significantly affected the expression of a large number of mRNAs involved in cancer, embryonic development and the glutathione system. Particularly notable was the extreme deficiency of glutaredoxin 1 (GLRX1) and glutathione transferase Omega 1 (GSTO1). To assess these phenotypes at the cellular level, we targeted the removal of SPS1 in F9 cells, a mouse embryonal carcinoma (EC) cell line, which affected the glutathione system proteins and accordingly led to the accumulation of hydrogen peroxide in the cell. Furthermore, we found that several malignant characteristics of SPS1-deficient F9 cells were reversed, suggesting that SPS1 played a role in supporting and/or sustaining cancer. In addition, the overexpression of mouse or human GLRX1 led to a reversal of observed increases in reactive oxygen species (ROS) in the F9 SPS1/GLRX1-deficient cells and resulted in levels that were similar to those in F9 SPS1-sufficient cells. The results suggested that SPS1 is an essential mammalian enzyme with roles in regulating redox homoeostasis and controlling cell growth.
C1 [Tobe, Ryuta; Carlson, Bradley A.; Xu, Xue-Ming; Beaglehole, Daniel; Hatfield, Dolph L.] NIH, Mol Biol Selenium, Mouse Canc Genet Program, Ctr Canc Res, Bldg 10, Bethesda, MD 20892 USA.
[Huh, Jang Hoe; Bang, Jeyoung; Na, Ji-Woon; Kong, Young-Yun; Lee, Byeong Jae] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea.
[Castro, Nadia P.; Salomon, David S.] NIH, Tumor Growth Factor Sect, Mouse Canc Genet Program, Ctr Canc Res, Bldg 10, Bethesda, MD 20892 USA.
[Tsuji, Petra A.] Towson Univ, Dept Biol Sci, Towson, MD 21252 USA.
[Lee, Sang-Goo; Gladyshev, Vadim N.] Harvard Med Sch, Brigham & Womens Hosp, Div Genet, Boston, MA 02115 USA.
[Southon, Eileen] NCI Frederick, Basic Sci Program, SAIC Frederick, Frederick, MD 21702 USA.
[Seifried, Harold] NCI, Nutr Sci Res Grp, Bethesda, MD 20892 USA.
[Tessarollo, Lino] NIH, Neural Dev Sect, Mouse Canc Genet Program, Ctr Canc Res, Bldg 10, Bethesda, MD 20892 USA.
[Schweizer, Ulrich] Rhein Friedrich Wilhelms Univ Bonn, Inst Biochem & Mol Biol, D-53115 Bonn, Germany.
[Xu, Xue-Ming] Genecopoeia Inc, 9620 Med Ctr Dr 101, Rockville, MD 20850 USA.
RP Hatfield, DL (reprint author), NIH, Mol Biol Selenium, Mouse Canc Genet Program, Ctr Canc Res, Bldg 10, Bethesda, MD 20892 USA.; Lee, BJ (reprint author), Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea.
EM hatfield@mail.nih.gov; imbglmg@snu.ac.kr
OI Schweizer, Ulrich/0000-0003-1380-4780
FU Basic Science Research Program through National Research Foundation of
Korea (NRF) - Ministry of Education [2015R1D1A1A01059170]; Bio & Medical
Technology Development Program of the National Research Foundation
(NRF); Ministry of Science [2012M3A9D1054622]; Intramural Research
Program of the National Institutes of Health; National Institutes of
Health [CA080946, GM061603, GM065204]; National Cancer Institute Center
for Cancer Research; Deutsche Forschungsgemeinschaft (DFG)
[SCHW914/2-1]; Towson University's Jess and Mildred Fisher College of
Science and Mathematics Endowed Chair
FX This work was supported by the Basic Science Research Program through
the National Research Foundation of Korea (NRF) funded by the Ministry
of Education [grant number 2015R1D1A1A01059170]; the Bio & Medical
Technology Development Program of the National Research Foundation
(NRF), awarded by the Ministry of Science [grant number 2012M3A9D1054622
(to B.J.L.)]; the Intramural Research Program of the National Institutes
of Health; the National Institutes of Health [grant numbers CA080946,
GM061603 and GM065204 (to V.N.G.)]; the National Cancer Institute Center
for Cancer Research (to D.L.H., D.S.S. and L.T.); the Deutsche
Forschungsgemeinschaft (DFG) [grant number SCHW914/2-1 (to U.S.)]; and
the Towson University's Jess and Mildred Fisher College of Science and
Mathematics Endowed Chair (to P.A.T.).
NR 46
TC 0
Z9 0
U1 1
U2 1
PU PORTLAND PRESS LTD
PI LONDON
PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND
SN 0264-6021
EI 1470-8728
J9 BIOCHEM J
JI Biochem. J.
PD JUL
PY 2016
VL 473
BP 2141
EP 2154
DI 10.1042/BCJ20160393
PN 14
PG 14
WC Biochemistry & Molecular Biology
SC Biochemistry & Molecular Biology
GA EK1TA
UT WOS:000393707500014
PM 27208177
ER
PT J
AU Shen, HM
Li, Z
AF Shen, Hongmei
Li, Zheng
TI miRNAs in NMDA receptor-dependent synaptic plasticity and psychiatric
disorders
SO CLINICAL SCIENCE
LA English
DT Review
DE miRNA; NMDA receptor; psychiatric disorders; synaptic plasticity
ID LONG-TERM DEPRESSION; GLUTAMATE METABOTROPIC RECEPTORS; AUTISM SPECTRUM
DISORDERS; 22Q11.2 DELETION SYNDROME; ADULT-MOUSE FOREBRAIN; COPY-NUMBER
VARIATION; HIPPOCAMPAL-NEURONS; GENE-EXPRESSION; MICRORNA TARGETS;
MORPHOLOGICAL PLASTICITY
AB The identification and functional delineation of miRNAs (a class of small non-coding RNAs) have added a new layer of complexity to our understanding of the molecular mechanisms underlying synaptic plasticity. Genome-wide association studies in conjunction with investigations in cellular and animal models, moreover, provide evidence that miRNAs are involved in psychiatric disorders. In the present review, we examine the current knowledge about the roles played by miRNAs in NMDA (N-methyl-D-aspartate) receptor-dependent synaptic plasticity and psychiatric disorders.
C1 [Shen, Hongmei; Li, Zheng] NIMH, Sect Synapse Dev & Plast, NIH, Bethesda, MD 20892 USA.
RP Li, Z (reprint author), NIMH, Sect Synapse Dev & Plast, NIH, Bethesda, MD 20892 USA.
EM lizheng2@mail.nih.gov
NR 124
TC 0
Z9 0
U1 1
U2 1
PU PORTLAND PRESS LTD
PI LONDON
PA CHARLES DARWIN HOUSE, 12 ROGER STREET, LONDON WC1N 2JU, ENGLAND
SN 0143-5221
EI 1470-8736
J9 CLIN SCI
JI Clin. Sci.
PD JUL 1
PY 2016
VL 130
IS 14
BP 1137
EP 1146
DI 10.1042/CS20160046
PG 10
WC Medicine, Research & Experimental
SC Research & Experimental Medicine
GA EK2MI
UT WOS:000393761000001
PM 27252401
ER
PT J
AU Drelon, C
Berthon, A
Mathieu, M
Ragazzon, B
Kuick, R
Tabbal, H
Septier, A
Rodriguez, S
Batisse-Lignier, M
Sahut-Barnola, I
Dumontet, T
Pointud, JC
Lefrancois-Martinez, AM
Baron, S
Giordano, TJ
Bertherat, J
Martinez, A
Val, P
AF Drelon, Coralie
Berthon, Annabel
Mathieu, Mickael
Ragazzon, Bruno
Kuick, Rork
Tabbal, Houda
Septier, Amandine
Rodriguez, Stephanie
Batisse-Lignier, Marie
Sahut-Barnola, Isabelle
Dumontet, Typhanie
Pointud, Jean-Christophe
Lefrancois-Martinez, Anne-Marie
Baron, Silvere
Giordano, Thomas J.
Bertherat, Jerome
Martinez, Antoine
Val, Pierre
TI EZH2 is overexpressed in adrenocortical carcinoma and is associated with
disease progression
SO HUMAN MOLECULAR GENETICS
LA English
DT Article
ID HISTONE METHYLTRANSFERASE EZH2; BETA-CATENIN; PROSTATE-CANCER; PRB-E2F
PATHWAY; GENE-EXPRESSION; TUMORS; CELLS; METHYLATION; ACTIVATION;
REVEALS
AB Adrenal Cortex Carcinoma (ACC) is an aggressive tumour with poor prognosis. Common alterations in patients include constitutive WNT/beta-catenin signalling and overexpression of the growth factor IGF2. However, the combination of both alterations in transgenic mice is not sufficient to trigger malignant tumour progression, suggesting that other alterations are required to allow development of carcinomas. Here, we have conducted a study of publicly available gene expression data from three cohorts of ACC patients to identify relevant alterations. Our data show that the histone methyltransferase EZH2 is overexpressed in ACC in the three cohorts. This overexpression is the result of deregulated P53/RB/E2F pathway activity and is associated with increased proliferation and poorer prognosis in patients. Inhibition of EZH2 by RNA interference or pharmacological treatment with DZNep inhibits cellular growth, wound healing and clonogenic growth and induces apoptosis of H295R cells in culture. Further growth inhibition is obtained when DZNep is combined with mitotane, the gold-standard treatment for ACC. Altogether, these observations suggest that overexpression of EZH2 is associated with aggressive progression and may constitute an interesting therapeutic target in the context of ACC.
C1 [Drelon, Coralie; Berthon, Annabel; Mathieu, Mickael; Tabbal, Houda; Septier, Amandine; Rodriguez, Stephanie; Batisse-Lignier, Marie; Sahut-Barnola, Isabelle; Dumontet, Typhanie; Pointud, Jean-Christophe; Lefrancois-Martinez, Anne-Marie; Baron, Silvere; Martinez, Antoine; Val, Pierre] Clermont Univ, INSERM, U1103, CNRS,GReD,UMR6293, F-63001 Clermont Ferrand, France.
[Berthon, Annabel] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Bethesda, MD 20892 USA.
[Ragazzon, Bruno; Bertherat, Jerome] Univ Paris 05, INSERM, U1016, Inst Cochin,CNRS,UMR 8104, Paris, France.
[Kuick, Rork] Univ Michigan Hlth Syst, Dept Biostat, Ann Arbor, MI 48109 USA.
[Batisse-Lignier, Marie] Ctr Hosp Univ, Fac Med, Serv Endocrinol, F-63000 Clermont Ferrand, France.
[Giordano, Thomas J.] Univ Michigan Hlth Syst, Dept Pathol, Ann Arbor, MI 48109 USA.
RP Val, P (reprint author), GReD, CNRS, UMR 6293, 24 Ave Landais, F-63171 Aubiere, France.
EM pierre.val@univ-bpclermont.fr
RI Ragazzon, Bruno/E-6541-2017
OI Ragazzon, Bruno/0000-0001-9476-4973
FU Centre National de la Recherche Scientifique; Institut National de la
Sante et de la Recherche Medicale; Universite Blaise Pascal; Universite
d'Auvergne; Fondation de France; La Ligue Contre le Cancer (Allier & Puy
de Dome committees); Fondation ARC [PJA20141201894]; Societe Francaise
d'Endocrinologie; University of Michigan Cancer Center [P30-CA046592]
FX This work was supported by institutional support from Centre National de
la Recherche Scientifique, Institut National de la Sante et de la
Recherche Medicale, Universite Blaise Pascal, Universite d'Auvergne and
through grants from Fondation de France, La Ligue Contre le Cancer
(Allier & Puy de Dome committees), Fondation ARC (PJA20141201894),
Societe Francaise d'Endocrinologie (young researcher grant to C.D. and
Researcher Prize to P.V.) and University of Michigan Cancer Center
Support Grant P30-CA046592 (NIH to R.K. and T.J.G.). The funders had no
role in study design, data collection and analysis, decision to publish
or preparation of the article.
NR 51
TC 0
Z9 0
U1 2
U2 2
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0964-6906
EI 1460-2083
J9 HUM MOL GENET
JI Hum. Mol. Genet.
PD JUL 1
PY 2016
VL 25
IS 13
BP 2789
EP 2800
DI 10.1093/hmg/ddw136
PG 12
WC Biochemistry & Molecular Biology; Genetics & Heredity
SC Biochemistry & Molecular Biology; Genetics & Heredity
GA EJ2TP
UT WOS:000393064400014
PM 27149985
ER
PT J
AU Leslie, EJ
Carlson, JC
Shaffer, JR
Feingold, E
Wehby, G
Laurie, CA
Jain, D
Laurie, CC
Doheny, KF
McHenry, T
Resick, J
Sanchez, C
Jacobs, J
Emanuele, B
Vieira, AR
Neiswanger, K
Lidral, AC
Valencia-Ramirez, LC
Lopez-Palacio, AM
Valencia, DR
Arcos-Burgos, M
Czeizel, AE
Field, LL
Padilla, CD
Maria, E
Cutiongco-de la Paz, C
Deleyiannis, F
Christensen, K
Munger, RG
Lie, RT
Wilcox, A
Romitti, PA
Castilla, EE
Mereb, JC
Poletta, FA
Orioli, IM
Carvalho, FM
Hecht, JT
Blanton, SH
Buxo, CJ
Butali, A
Mossey, PA
Adeyemo, WL
James, O
Braimah, RO
Aregbesola, BS
Eshete, MA
Abate, F
Koruyucu, M
Seymen, F
Ma, L
de Salamanca, JE
Weinberg, SM
Moreno, L
Murray, JC
Marazita, ML
AF Leslie, Elizabeth J.
Carlson, Jenna C.
Shaffer, John R.
Feingold, Eleanor
Wehby, George
Laurie, Cecelia A.
Jain, Deepti
Laurie, Cathy C.
Doheny, Kimberly F.
McHenry, Toby
Resick, Judith
Sanchez, Carla
Jacobs, Jennifer
Emanuele, Beth
Vieira, Alexandre R.
Neiswanger, Katherine
Lidral, Andrew C.
Consuelo Valencia-Ramirez, Luz
Maria Lopez-Palacio, Ana
Rivera Valencia, Dora
Arcos-Burgos, Mauricio
Czeizel, Andrew E.
Field, L. Leigh
Padilla, Carmencita D.
Maria, Eva
Cutiongco-de la Paz, C.
Deleyiannis, Frederic
Christensen, Kaare
Munger, Ronald G.
Lie, Rolv T.
Wilcox, Allen
Romitti, Paul A.
Castilla, Eduardo E.
Mereb, Juan C.
Poletta, Fernando A.
Orioli, Ieda M.
Carvalho, Flavia M.
Hecht, Jacqueline T.
Blanton, Susan H.
Buxo, Carmen J.
Butali, Azeez
Mossey, Peter A.
Adeyemo, Wasiu L.
James, Olutayo
Braimah, Ramat O.
Aregbesola, Babatunde S.
Eshete, Mekonen A.
Abate, Fikre
Koruyucu, Mine
Seymen, Figen
Ma, Lian
Enriquez de Salamanca, Javier
Weinberg, Seth M.
Moreno, Lina
Murray, Jeffrey C.
Marazita, Mary L.
TI A multi-ethnic genome-wide association study identifies novel loci for
non-syndromic cleft lip with or without cleft palate on 2p24.2, 17q23
and 19q13
SO HUMAN MOLECULAR GENETICS
LA English
DT Article
ID SUSCEPTIBILITY LOCUS; CAUSE VAN; GENE; RISK; POPULATION; EXPRESSION;
MUTATIONS; VARIANTS; CANCER; ENDOTHELIN-1
AB Orofacial clefts (OFCs), which include non-syndromic cleft lip with or without cleft palate (CL/P), are among the most common birth defects in humans, affecting approximately 1 in 700 newborns. CL/P is phenotypically heterogeneous and has a complex etiology caused by genetic and environmental factors. Previous genome-wide association studies (GWASs) have identified at least 15 risk loci for CL/P. As these loci do not account for all of the genetic variance of CL/P, we hypothesized the existence of additional risk loci. We conducted am ultiethnic GWAS in 6480 participants (823 unrelated cases, 1700 unrelated controls and 1319 case-parent trios) with European, Asian, African and Central and South American ancestry. Our GWAS revealed novel associations on 2p24 near FAM49A, a gene of unknown function (P = 4.22 x 10(-8)), and 19q13 near RHPN2, a gene involved in organizing the actin cytoskeleton (P = 4.17 x 10(-8)). Other regions reaching genome-wide significance were 1p36 (PAX7), 1p22 (ARHGAP29), 1q32 (IRF6), 8q24 and 17p13 (NTN1), all reported in previous GWASs. Stratification by ancestry group revealed a novel association with a region on 17q23 (P = 2.92 x 10(-8)) among individuals with European ancestry. This region included several promising candidates including TANC2, an oncogene required for development, and DCAF7, a scaffolding protein required for craniofacial development. In the Central and South American ancestry group, significant associations with loci previously identified in Asian or European ancestry groups reflected their admixed ancestry. In summary, we have identified novel CL/P risk loci and suggest new genes involved in craniofacial development, confirming the highly heterogeneous etiology of OFCs.
C1 [Leslie, Elizabeth J.; Carlson, Jenna C.; Shaffer, John R.; Feingold, Eleanor; McHenry, Toby; Resick, Judith; Sanchez, Carla; Jacobs, Jennifer; Emanuele, Beth; Vieira, Alexandre R.; Neiswanger, Katherine; Weinberg, Seth M.; Marazita, Mary L.] Univ Pittsburgh, Ctr Craniofacial & Dent Genet, Dept Oral Biol, Sch Dent Med, Pittsburgh, PA 15219 USA.
[Carlson, Jenna C.; Feingold, Eleanor] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Biostat, Pittsburgh, PA 15261 USA.
[Shaffer, John R.; Feingold, Eleanor; Vieira, Alexandre R.; Marazita, Mary L.] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Human Genet, Pittsburgh, PA 15261 USA.
[Wehby, George] Univ Iowa, Coll Publ Hlth, Dept Hlth Management & Policy, Iowa City, IA 52246 USA.
[Laurie, Cecelia A.; Jain, Deepti; Laurie, Cathy C.] Univ Washington, Dept Biostat, Genet Coordinating Ctr, Seattle, WA 98195 USA.
[Doheny, Kimberly F.] Johns Hopkins Univ, Ctr Inherited Dis Res, Baltimore, MD 21224 USA.
[Lidral, Andrew C.; Moreno, Lina] Univ Iowa, Dept Orthodont, Coll Publ Hlth, Iowa City, IA 52242 USA.
[Romitti, Paul A.] Univ Iowa, Dept Epidemiol, Coll Publ Hlth, Iowa City, IA 52242 USA.
[Butali, Azeez; Marazita, Mary L.] Univ Iowa, Coll Dent, Dept Oral Pathol Radiol & Med, Dows Inst Dent Res, Iowa City, IA 52242 USA.
[Murray, Jeffrey C.] Univ Iowa, Dept Pediat, Carver Coll Med, Iowa City, IA 52242 USA.
[Consuelo Valencia-Ramirez, Luz] Fdn Clin Noel, Medellin 050012, Colombia.
[Maria Lopez-Palacio, Ana] Univ Antioquia, Dept Basic Integrated Studies, Coll Dent, Medellin 050001, Colombia.
[Rivera Valencia, Dora] Univ Antioquia, Populat Genet & Mutacarcinogenesis Grp, Medellin 050001, Colombia.
[Arcos-Burgos, Mauricio] Australian Natl Univ, ANU Coll Med Biol & Environm, John Curtin Sch Med Res, Genom & Predict Med,Genome Biol Dept, Canberra, ACT 0200, Australia.
[Czeizel, Andrew E.] Fdn Community Control Hereditary Dis, H-1051 Budapest, Hungary.
[Field, L. Leigh] Univ British Columbia, Dept Med Genet, Vancouver, BC V6H 3N1, Canada.
[Padilla, Carmencita D.; Cutiongco-de la Paz, C.] Coll Med, Dept Pediat, Manila 1000, Philippines.
[Padilla, Carmencita D.; Cutiongco-de la Paz, C.] Natl Inst Hlth, Inst Human Genet, Manila 1000, Philippines.
[Padilla, Carmencita D.; Cutiongco-de la Paz, C.] Univ Philippines Manila, Manila 1000, Philippines.
[Padilla, Carmencita D.; Cutiongco-de la Paz, C.] Univ Philippines Syst, Philippine Genome Ctr, Manila 1101, Philippines.
[Deleyiannis, Frederic] Univ Colorado, Dept Surg Plast & Reconstruct Surg, Sch Med, Denver, CO 80045 USA.
[Christensen, Kaare] Univ Southern Denmark, Dept Epidemiol, Inst Publ Hlth, DK-5230 Odense, Denmark.
[Munger, Ronald G.] Utah State Univ, Dept Nutr Dietet & Food Sci, Logan, UT 84322 USA.
[Lie, Rolv T.] Univ Bergen, Dept Global Publ Hlth & Primary Care, NO-5020 Bergen, Norway.
[Wilcox, Allen] NIEHS, Epidemiol Branch, Res Triangle Pk, NC 27709 USA.
[Castilla, Eduardo E.; Poletta, Fernando A.] CEMIC Ctr Med Educ & Clin Res, RA-1431 Buenos Aires, DF, Argentina.
[Castilla, Eduardo E.; Poletta, Fernando A.; Carvalho, Flavia M.] Fiocruz MS, Inst Oswaldo Cruz, Lab Congenital Malformat Epidemiol, BR-21040360 Rio De Janeiro, Brazil.
[Castilla, Eduardo E.; Poletta, Fernando A.; Orioli, Ieda M.; Carvalho, Flavia M.] Univ Fed Rio de Janeiro, ECLAMC Latin Amer Collaborat Study Congenital Mal, INAGEMP Natl Inst Populat Med Genet, BR-21941617 Rio De Janeiro, Brazil.
[Orioli, Ieda M.] Univ Fed Rio de Janeiro, Dept Genet, Inst Biol, BR-21941617 Rio De Janeiro, Brazil.
[Mereb, Juan C.] Hosp Area, ECLAMC Latin Amer Collaborat Study Congenital Mal, RA-8430 El Bolson, Argentina.
[Hecht, Jacqueline T.] Univ Texas Hlth Sci Ctr Houston, Dept Pediat, Houston, TX 77030 USA.
[Blanton, Susan H.] Univ Miami, Hussman Inst Human Gen, Mailman Sch Med, Dr John T Macdonald Fdn,Dept Human Genet, Coral Gables, FL 33124 USA.
[Buxo, Carmen J.] Univ Puerto Rico, Sch Dent Med, San Juan, PR 00936 USA.
[Mossey, Peter A.] Univ Dundee, Dept Orthodont, Dundee DD1 4HN, Scotland.
[Adeyemo, Wasiu L.; James, Olutayo] Univ Lagos, Coll Med, Dept Oral & Maxillofacial Surg, PMB 12003, Lagos, Nigeria.
[Braimah, Ramat O.; Aregbesola, Babatunde S.] Obafemi Awolowo Univ, Dept Oral & Maxillofacial Surg, PMB 13, Ife, Nigeria.
[Eshete, Mekonen A.; Abate, Fikre] Univ Addis Ababa, Sch Med, Dept Surg, POB 26493, Addis Ababa, Ethiopia.
[Koruyucu, Mine; Seymen, Figen] Istanbul Univ, Dept Pedodont, TR-34116 Istanbul, Turkey.
[Ma, Lian] Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China.
[Enriquez de Salamanca, Javier] Hosp Infantil Univ Nino Jesus, Unidad Cirugia Plast, Madrid 28009, Spain.
[Marazita, Mary L.] Univ Pittsburgh, Sch Med, Clin & Translat Sci, Pittsburgh, PA 15213 USA.
RP Marazita, ML (reprint author), Suite 500 Bridgeside Point,100 Technol Dr, Pittsburgh, PA 15219 USA.
EM marazita@pitt.edu
OI Wilcox, Allen/0000-0002-3376-1311; Christensen,
Kaare/0000-0002-5429-5292
FU National Institutes of Health [X01-HG007485, R01-DE016148, U01-DE024425,
R37-DE008559, R01-DE009886, R01-DE014667, R21-DE016930, R01-DE012472,
R01-DE011931, R01-DE011948, R01-DD000295, U54-MD007587]; Robert Wood
Johnson Foundation, AMFDP [72429]; Research Institute of the Children's
Hospital of Colorado (FWD); Institute of Human Genetics, National
Institutes of Health, University of the Philippines, Manila; Fundacao de
Amparo a Pesquisa do Estado do Rio de Janeiro, Brazil
[E-26/102.797/2012, E-26/110.140/2013]; CNPq, Brazil [481069/2012-7,
306396/2013-0, 400427/2013-3]; Danish National Research Foundation;
Pharmacy Foundation; Egmont Foundation; March of Dimes Birth Defects
Foundation; Augustinus Foundation; Health Foundation; National Institute
of Environmental Health Sciences/National Institutes of Health
Intramural Research Program; Norwegian Research Council; The National
Institutes of Health [R25-MD007607, K99-DE024571, K99/R00-DE022378,
K99-DE025060, R01-DE020895, HHSN268201200008I]
FX National Institutes of Health (X01-HG007485 to MLM, R01-DE016148 to
M.L.M., U01-DE024425 to M.L.M, R37-DE008559 to J.C.M. and MLM,
R01-DE009886 to M.L.M., R21-DE016930 to M.L.M., R01-DE014667 to A.C.L.
and L.M.M., R21-DE016930 to M.L.M., R01-DE012472 to M.L.M., R01-DE011931
to J.T.H., R01-DE011948 to K.C., R01-DD000295 to G.L.W., U54-MD007587 to
C.J.B., R25-MD007607 to C.J.B., K99-DE024571 to C.J.B., K99/R00-DE022378
to A.B., K99-DE025060 to E.J.L., and R01-DE020895 to G.L.W.). Genotyping
and data cleaning were provided via an National Institutes of Health
contract HHSN268201200008I to the Johns Hopkins Center for Inherited
Disease Research. Additional support provided by: the Robert Wood
Johnson Foundation, AMFDP Grant (72429 to A.B.); an intramural grant
from the Research Institute of the Children's Hospital of Colorado
(FWD); operating costs support in the Philippines was provided by the
Institute of Human Genetics, National Institutes of Health, University
of the Philippines, Manila (C.P.); grants through Fundacao de Amparo a
Pesquisa do Estado do Rio de Janeiro, Brazil (I.M.O.): grant numbers
(E-26/102.797/2012, E-26/110.140/2013); grants through CNPq, Brazil
(I.M.O.): grant numbers: (481069/2012-7, 306396/2013-0, 400427/2013-3).
Some replication samples were from the Utah Child and Family Health
study (R01-DE016877 [R.G.M.]. Some replication samples were from the
Danish National Birth Cohort (PIs Mads Melbye and Jorn Olsen) funded by
a major grant from the Danish National Research Foundation and initiated
by the Danish Epidemiology Science Centre. Additional support for the
Danish National Birth Cohort was obtained from the Pharmacy Foundation,
the Egmont Foundation, the March of Dimes Birth Defects Foundation, the
Augustinus Foundation, and the Health Foundation. Some replication
samples were from the Norwegian Cleft Study and from the Norwegian MoBa
Study, both supported by the National Institute of Environmental Health
Sciences/National Institutes of Health Intramural Research Program and
the Norwegian Research Council.
NR 52
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Z9 5
U1 1
U2 1
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0964-6906
EI 1460-2083
J9 HUM MOL GENET
JI Hum. Mol. Genet.
PD JUL 1
PY 2016
VL 25
IS 13
BP 2862
EP 2872
DI 10.1093/hmg/ddw104
PG 11
WC Biochemistry & Molecular Biology; Genetics & Heredity
SC Biochemistry & Molecular Biology; Genetics & Heredity
GA EJ2TP
UT WOS:000393064400020
PM 27033726
ER
PT J
AU Yale, K
Tackett, AJ
Neuman, M
Bulley, E
Chait, BT
Wiley, E
AF Yale, Katerina
Tackett, Alan J.
Neuman, Monica
Bulley, Emily
Chait, Brian T.
Wiley, Emily
TI Phosphorylation-Dependent Targeting of Tetrahymena HP1 to Condensed
Chromatin
SO MSPHERE
LA English
DT Article
DE HP1; Tetrahymena; chromatin; chromodomain; ciliates; heterochromatin;
protein phosphorylation
ID HETEROCHROMATIN PROTEIN-1 HP1; HISTONE H3; HP1-LIKE PROTEIN; DNA
ELIMINATION; SHADOW DOMAIN; DROSOPHILA-MELANOGASTER; MACRONUCLEAR
ANLAGEN; GENE-EXPRESSION; CELL-CYCLE; BINDING
AB The evolutionarily conserved proteins related to heterochromatin protein 1 (HP1), originally described in Drosophila, are well known for their roles in heterochromatin assembly and gene silencing. Targeting of HP1 proteins to specific chromatin locales is mediated, at least in part, by the HP1 chromodomain, which binds to histone H3 methylated at lysine 9 that marks condensed regions of the genome. Mechanisms that regulate HP1 targeting are emerging from studies with yeast and metazoans and point to roles for posttranslational modifications. Here, we report that modifications of an HP1 homolog (Hhp1) in the ciliate model Tetrahymena thermophila correlated with the physiological state and with nuclear differentiation events involving the restructuring of chromatin. Results support the model in which Hhp1 chromodomain binds lysine 27-methylated histone H3, and we show that colocalization with this histone mark depends on phosphorylation at a single Cdc2/Cdk1 kinase site in the "hinge region" adjacent to the chromodomain. These findings help elucidate important functional roles of reversible posttranslational modifications of proteins in the HP1 family, in this case, regulating the targeting of a ciliate HP1 to chromatin regions marked with methylated H3 lysine 27.
IMPORTANCE Compacting the genome to various degrees influences processes that use DNA as a template, such as gene transcription and replication. This project was aimed at learning more about the cellular mechanisms that control genome compaction. Posttranslational modifications of proteins involved in genome condensation are emerging as potentially important points of regulation. To help elucidate protein modifications and how they affect the function of condensation proteins, we investigated the phosphorylation of the chromatin protein called Hhp1 in the ciliated protozoan Tetrahymena thermophila. This is one of the first functional investigations of these modifications of a nonhistone chromatin condensation protein that acts on the ciliate genome, and discoveries will aid in identifying common, evolutionarily conserved strategies that control the dynamic compaction of genomes.
C1 [Yale, Katerina; Neuman, Monica; Bulley, Emily; Wiley, Emily] Pitzer, WM Keck Sci Ctr Claremont McKenna, Claremont, CA 91711 USA.
[Yale, Katerina; Neuman, Monica; Bulley, Emily; Wiley, Emily] Scripps Coll, Claremont, CA 91711 USA.
[Tackett, Alan J.; Chait, Brian T.] Rockefeller Univ, Lab Mass Spectrometry & Gaseous Ion Chem, 1230 York Ave, New York, NY 10021 USA.
[Yale, Katerina] George Washington Univ, Sch Med & Hlth Sci, Washington, DC 63130 USA.
[Tackett, Alan J.] Univ Arkansas Med Sci, Little Rock, AR 72205 USA.
[Neuman, Monica] NIH, Bethesda, MD USA.
[Wiley, Emily] Univ Washington, Sch Med, Seattle, WA USA.
EM ewiley@kecksci.claremont.edu
FU HHS \ National Institutes of Health (NIH) [GM18785-04, GM103314];
National Science Foundation (NSF) [MCB 0545560]
FX This work, including the efforts of Emily Wiley, was funded by HHS
vertical bar National Institutes of Health (NIH) (GM18785-04). This
work, including the efforts of Brian T. Chait, was funded by HHS
vertical bar National Institutes of Health (NIH) (GM103314). This work,
including the efforts of Emily Wiley, was funded by National Science
Foundation (NSF) (MCB 0545560).
NR 59
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Z9 0
U1 1
U2 1
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2379-5042
J9 MSPHERE
JI mSphere
PD JUL-AUG
PY 2016
VL 1
IS 4
AR e00142-16
DI 10.1128/mSphere.00142-16
PG 14
WC Microbiology
SC Microbiology
GA EI6DU
UT WOS:000392586000010
ER
PT J
AU Bohon, KS
Hermann, KL
Hansen, T
Conway, BR
AF Bohon, Kaitlin S.
Hermann, Katherine L.
Hansen, Thorsten
Conway, Bevil R.
TI Representation of Perceptual Color Space in Macaque Posterior Inferior
Temporal Cortex (the V4 Complex)
SO ENEURO
LA English
DT Article
DE color; macaque; monkey; neurophysiology; v4; vision
ID PRIMARY VISUAL-CORTEX; LATERAL GENICULATE-NUCLEUS; UNIQUE HUES;
CHROMATIC MECHANISMS; STRIATE CORTEX; AREA V4; EXTRASTRIATE CORTEX;
CARDINAL DIRECTIONS; SELECTIVE CELLS; CONE SIGNALS
AB The lateral geniculate nucleus is thought to represent color using two populations of cone-opponent neurons [ L vs M; S vs (L + M)], which establish the cardinal directions in color space (reddish vs cyan; lavender vs lime). How is this representation transformed to bring about color perception? Prior work implicates populations of glob cells in posterior inferior temporal cortex (PIT; the V4 complex), but the correspondence between the neural representation of color in PIT/V4 complex and the organization of perceptual color space is unclear. We compared color-tuning data for populations of glob cells and interglob cells to predictions obtained using models that varied in the color-tuning narrowness of the cells, and the color preference distribution across the populations. Glob cells were best accounted for by simulated neurons that have nonlinear (narrow) tuning and, as a population, represent a color space designed to be perceptually uniform (CIELUV). Multidimensional scaling and representational similarity analyses showed that the color space representations in both glob and interglob populations were correlated with the organization of CIELUV space, but glob cells showed a stronger correlation. Hue could be classified invariant to luminance with high accuracy given glob responses and above-chance accuracy given interglob responses. Luminance could be read out invariant to changes in hue in both populations, but interglob cells tended to prefer stimuli having luminance contrast, regardless of hue, whereas glob cells typically retained hue tuning as luminance contrast was modulated. The combined luminance/hue sensitivity of glob cells is predicted for neurons that can distinguish two colors of the same hue at different luminance levels (orange/brown).
C1 [Bohon, Kaitlin S.; Hermann, Katherine L.; Conway, Bevil R.] Wellesley Coll, Program Neurosci, Wellesley, MA 02481 USA.
[Bohon, Kaitlin S.; Hermann, Katherine L.; Conway, Bevil R.] MIT, Dept Brain & Cognit Sci, E25-618, Cambridge, MA 02139 USA.
[Hansen, Thorsten] Justus Liebig Univ Giessen, Dept Psychol, D-35396 Giessen, Germany.
RP Conway, BR (reprint author), NEI, Sensorimotor Res Lab, NIH, Bldg 10, Bethesda, MD 20892 USA.
EM bevil@mit.edu
FU National Institutes of Health [EY023322]; National Science Foundation
[1353571]
FX This research was supported by National Institutes of Health Grant
EY023322 and National Science Foundation Grant 1353571.
NR 102
TC 1
Z9 1
U1 1
U2 1
PU SOC NEUROSCIENCE
PI WASHINGTON
PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA
SN 2373-2822
J9 ENEURO
JI eNeuro
PD JUL-AUG
PY 2016
VL 3
IS 4
DI 10.1523/ENEURO.0039-16.2016
PG 28
WC Neurosciences
SC Neurosciences & Neurology
GA EH7BV
UT WOS:000391928700005
ER
PT J
AU Cheng, N
Jiao, S
Gumaste, A
Bai, L
Belluscio, L
AF Cheng, Ning
Jiao, Song
Gumaste, Ankita
Bai, Li
Belluscio, Leonardo
TI APP Overexpression Causes A beta-Independent Neuronal Death through
Intrinsic Apoptosis Pathway
SO ENEURO
LA English
DT Article
DE amyloid precursor protein; apoptosis; neurodegeneration; olfactory
ID MODERATE ALZHEIMERS-DISEASE; NEURODEGENERATIVE DISEASES; PATHOLOGICAL
FEATURES; AMYLOID DEPOSITION; GENE-EXPRESSION; CELL-DEATH; KINASE-II;
MODEL; SECRETASE; MOUSE
AB Accumulation of amyloid-beta (A beta) peptide in the brain is a central hallmark of Alzheimer's disease (AD) and is thought to be the cause of the observed neurodegeneration. Many animal models have been generated that overproduce A yet do not exhibit clear neuronal loss, questioning this A beta hypothesis. We previously developed an in vivo mouse model that expresses a humanized amyloid precursor protein (hAPP) in olfactory sensory neurons (OSNs) showing robust apoptosis and olfactory dysfunction by 3 weeks of age, which is consistent with early OSN loss and smell deficits, as observed in AD patients. Here we show, by deleting the beta-site APP cleaving enzyme 1 (BACE1) in two distinct transgenic mouse models, that hAPP-induced apoptosis of OSNs is A beta independent and remains cell autonomous. In addition, we reveal that the intrinsic apoptosis pathway is responsible for hAPP-induced OSN death, as marked by mitochondrial damage and caspase-9 activation. Given that hAPP expression causes OSN apoptosis despite the absence of BACE1, we propose that A beta is not the sole cause of hAPP-induced neurodegeneration and that the early loss of olfactory function in AD may be based on a cell-autonomous mechanism, which could mark an early phase of AD, prior to A beta accumulation. Thus, the olfactory system could serve as an important new platform to study the development of AD, providing unique insight for both early diagnosis and intervention.
C1 [Cheng, Ning; Jiao, Song; Gumaste, Ankita; Bai, Li; Belluscio, Leonardo] NINDS, Dev Neural Plast Sect, NIH, 35 Convent Dr, Bethesda, MD 20892 USA.
RP Belluscio, L (reprint author), NINDS, Dev Neural Plast Sect, NIH, 35 Convent Dr, Bethesda, MD 20892 USA.
EM belluscl@ninds.nih.gov
FU Intramural Research Program of the National Institute of Neurological
Disorders and Stroke
FX This research was supported by the Intramural Research Program of the
National Institute of Neurological Disorders and Stroke.
NR 46
TC 1
Z9 1
U1 1
U2 1
PU SOC NEUROSCIENCE
PI WASHINGTON
PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA
SN 2373-2822
J9 ENEURO
JI eNeuro
PD JUL-AUG
PY 2016
VL 3
IS 4
DI 10.1523/ENEURO.0150-16.2016
PG 12
WC Neurosciences
SC Neurosciences & Neurology
GA EH7BV
UT WOS:000391928700029
ER
PT J
AU Nordestgaard, BG
Langsted, A
Mora, S
Kolovou, G
Baum, H
Bruckert, E
Watts, GF
Sypniewska, G
Wiklund, O
Boren, J
Chapman, MJ
Cobbaert, C
Descamps, OS
von Eckardstein, A
Kamstrup, PR
Pulkki, K
Kronenberg, F
Remaley, AT
Rifai, N
Ros, E
Langlois, M
AF Nordestgaard, Borge G.
Langsted, Anne
Mora, Samia
Kolovou, Genovefa
Baum, Hannsjorg
Bruckert, Eric
Watts, Gerald F.
Sypniewska, Grazyna
Wiklund, Olov
Boren, Jan
Chapman, M. John
Cobbaert, Christa
Descamps, Olivier S.
von Eckardstein, Arnold
Kamstrup, Pia R.
Pulkki, Kari
Kronenberg, Florian
Remaley, Alan T.
Rifai, Nader
Ros, Emilio
Langlois, Michel
CA EAS
European Federation Clinical Chem
TI Fasting Is Not Routinely Required for Determination of a Lipid Profile:
Clinical and Laboratory Implications Including Flagging at Desirable
Concentration Cutpoints-A Joint Consensus Statement from the European
Atherosclerosis Society and European Federation of Clinical Chemistry
and Laboratory Medicine
SO CLINICAL CHEMISTRY
LA English
DT Article
ID DENSITY-LIPOPROTEIN CHOLESTEROL; ISCHEMIC-HEART-DISEASE;
GENERAL-POPULATION; FAMILIAL HYPERCHOLESTEROLEMIA; NONFASTING
TRIGLYCERIDES; CARDIOVASCULAR-DISEASE; ELEVATED LIPOPROTEIN(A);
MYOCARDIAL-INFARCTION; REMNANT CHOLESTEROL; RISK-FACTOR
AB AIMS: To critically evaluate the clinical implications of the use of non-fasting rather than fasting lipid profiles and to provide guidance for the laboratory reporting of abnormal non-fasting or fasting lipid profiles.
METHODS AND RESULTS: Extensive observational data, in which random non-fasting lipid profiles have been compared with those determined under fasting conditions, indicate that the maximal mean changes at 1-6 h after habitual meals are not clinically significant [+0.3 mmol/L (26 mg/dL) for triglycerides; -0.2 mmol/L (8 mg/dL) for total cholesterol; -0.2 mmol/L (8 mg/dL) for LDL cholesterol; +0.2 mmol/L (8 mg/dL) for calculated remnant cholesterol; -0.2 mmol/L (8 mg/dL) for calculated non-HDL cholesterol]; concentrations of HDL cholesterol, apolipoprotein A1, apolipoprotein B, and lipoprotein(a) are not affected by fasting/non-fasting status. In addition, non-fasting and fasting concentrations vary similarly over time and are comparable in the prediction of cardiovascular disease. To improve patient compliance with lipid testing, we therefore recommend the routine use of non-fasting lipid profiles, whereas fasting sampling may be considered when non-fasting triglycerides are > 5 mmol/L (440 mg/dL). For non-fasting samples, laboratory reports should flag abnormal concentrations as triglycerides >= 2 mmol/L (175 mg/dL), total cholesterol >= 5 mmol/L (190 mg/dL), LDL cholesterol >= 3 mmol/L (115 mg/dL), calculated remnant cholesterol >= 0.9 mmol/L (35 mg/dL), calculated non-HDL cholesterol >= 3.9 mmol/L (150 mg/dL), HDL cholesterol <= 1 mmol/L (40 mg/dL), apolipoprotein A1 <= 1.25 g/L (125 mg/dL), apolipoprotein B >= 1.0 g/L (100 mg/dL), and lipoprotein(a) >= 50 mg/dL (80th percentile); for fasting samples, abnormal concentrations correspond to triglycerides >= 1.7 mmol/L (150 mg/dL). Life-threatening concentrations require separate referral for the risk of pancreatitis when triglycerides are >10 mmol/L (880 mg/dL), for homozygous familial hyper-cholesterolemia when LDL cholesterol is >13 mmol/L (500 mg/dL), for heterozygous familial hypercholesterol-emia when LDL cholesterol is >5 mmol/L (190 mg/dL), and for very high cardiovascular risk when lipoprotein(a) > 150 mg/dL (99th percentile).
CONCLUSIONS: We recommend that non-fasting blood samples be routinely used for the assessment of plasma lipid profiles. Laboratory reports should flag abnormal values on the basis of desirable concentration cutpoints. Non-fasting and fasting measurements should be complementary but not mutually exclusive. (C) 2016 American Association for Clinical Chemistry
C1 [Nordestgaard, Borge G.; Langsted, Anne; Kamstrup, Pia R.] Univ Copenhagen, Dept Clin Biochem, Herlev & Gentofte Hosp, Copenhagen Univ Hosp, Herlev, Denmark.
[Mora, Samia] Harvard Med Sch, Brigham & Womens Hosp, Div Prevent Med, Boston, MA USA.
[Mora, Samia] Harvard Med Sch, Brigham & Womens Hosp, Div Cardiovasc Med, Boston, MA USA.
[Kolovou, Genovefa] Onassis Cardiac Surg Ctr, Dept Cardiol, Athens, Greece.
[Baum, Hannsjorg] Reg Kliniken Holding RKH GmbH, Inst Lab Med Blutdepot & Krankenhaushyg, Ludwigsburg, Germany.
[Bruckert, Eric] Pitie Salpetriere Univ Hosp, Paris, France.
[Watts, Gerald F.] Univ Western Australia, Perth, WA, Australia.
[Sypniewska, Grazyna] NC Univ, Coll Med, Dept Lab Med, Bydgoszcz, Poland.
[Wiklund, Olov; Boren, Jan] Sahlgrens Univ Hosp, Gothenburg, Sweden.
[Chapman, M. John] Pitie Salpetriere Univ Hosp, INSERM, U939, Paris, France.
[Cobbaert, Christa] Leiden Univ, Med Ctr, Dept Clin Chem & Lab Med, Leiden, Netherlands.
[Descamps, Olivier S.] Hop Jolimont, Haine St Paul, Belgium.
[von Eckardstein, Arnold] Univ Zurich Hosp, Inst Clin Chem, Zurich, Switzerland.
[Pulkki, Kari] Univ Eastern Finland, Dept Clin Chem, Kuopio, Finland.
[Kronenberg, Florian] Med Univ Innsbruck, Dept Med Genet Mol & Clin Pharmacol, Div Genet Epidemiol, Innsbruck, Austria.
[Remaley, Alan T.] NHLBI, Lipoprot Metab Sect, Cardiovasc Pulm Branch, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Rifai, Nader] Harvard Univ, Childrens Hosp, Lab Med, Boston, MA 02115 USA.
[Ros, Emilio] Hosp Clin Barcelona, Lipid Clin, Dept Endocrinol & Nutr, Inst Invest Biomed August Pi Sunyer, Barcelona, Spain.
[Ros, Emilio] Inst Salud Carlos III, Ciber Fisiopatol Obesidad & Nutr, Madrid, Spain.
[Langlois, Michel] Acad Hosp St Jan, Dept Lab Med, Brugge, Belgium.
[Langlois, Michel] Univ Ghent, Ghent, Belgium.
RP Nordestgaard, BG (reprint author), Copenhagen Univ Hosp, Dept Clin Biochem, Herlev & Gentofte Hosp, DK-2730 Herlev, Denmark.
EM boerge.nordestgaard@regionh.dk
RI Kronenberg, Florian/B-1736-2008
OI Kronenberg, Florian/0000-0003-2229-1120
FU Merck; Roche Diagnostics; Denka Seiken; European Atherosclerosis
Society; European Federation of Clinical Chemistry and Laboratory
Medicine
FX Supported by unrestricted educational grants to EAS and EFLM from Merck,
Roche Diagnostics, and Denka Seiken. These companies were not present at
the Joint Consensus Panel meetings, had no role in the design or content
of the joint consensus statement, and had no right to approve or
disapprove of the final document. Funding to pay the Open Access
publication charges for this article was provided by the European
Atherosclerosis Society and the European Federation of Clinical
Chemistry and Laboratory Medicine.
NR 47
TC 5
Z9 5
U1 6
U2 6
PU AMER ASSOC CLINICAL CHEMISTRY
PI WASHINGTON
PA 2101 L STREET NW, SUITE 202, WASHINGTON, DC 20037-1526 USA
SN 0009-9147
EI 1530-8561
J9 CLIN CHEM
JI Clin. Chem.
PD JUL
PY 2016
VL 62
IS 7
BP 930
EP 946
DI 10.1373/clinchem.2016.258897
PG 17
WC Medical Laboratory Technology
SC Medical Laboratory Technology
GA EF2CL
UT WOS:000390132000009
PM 27235445
ER
PT J
AU Gordon, LB
Kieran, MW
Kleinman, ME
Misteli, T
AF Gordon, Leslie B.
Kieran, Mark W.
Kleinman, Monica E.
Misteli, Tom
TI The decision-making process and criteria in selecting candidate drugs
for progeria clinical trials
SO EMBO MOLECULAR MEDICINE
LA English
DT Editorial Material
ID HUTCHINSON-GILFORD PROGERIA
AB Hutchinson-Gilford progeria syndrome (progeria) is an extremely rare premature aging disease with a population prevalence of 1 in 20 million. Nevertheless, propelled by the discovery of a causal mutation in the lamin A/C gene (LMNA) (De Sandre-Giovannoli et al, 2003; Eriksson et al, 2003) and strong patient advocacy (Gordon & Gordon, 2014), progeria has rapidly become a vibrant field of study, attracting a wide range of researchers from basic cell biologists to clinicians.
C1 [Gordon, Leslie B.; Kleinman, Monica E.] Boston Childrens Hosp, Dept Anesthesiol Perioperat & Pain Med, Boston, MA 02115 USA.
[Gordon, Leslie B.; Kleinman, Monica E.] Harvard Med Sch, Boston, MA 02115 USA.
[Gordon, Leslie B.] Brown Univ, Dept Pediat, Hasbro Childrens Hosp, Providence, RI 02912 USA.
[Gordon, Leslie B.] Brown Univ, Warren Alpert Med Sch, Providence, RI 02912 USA.
[Kieran, Mark W.] Dana Farber Canc Inst, Dana Farber Boston Childrens Canc & Blood Disorde, Boston, MA 02115 USA.
[Misteli, Tom] NCI, NIH, Bethesda, MD 20892 USA.
RP Gordon, LB (reprint author), Boston Childrens Hosp, Dept Anesthesiol Perioperat & Pain Med, Boston, MA 02115 USA.; Gordon, LB (reprint author), Harvard Med Sch, Boston, MA 02115 USA.; Gordon, LB (reprint author), Brown Univ, Dept Pediat, Hasbro Childrens Hosp, Providence, RI 02912 USA.; Gordon, LB (reprint author), Brown Univ, Warren Alpert Med Sch, Providence, RI 02912 USA.
EM leslie_gordon@brown.edu
NR 8
TC 0
Z9 0
U1 1
U2 1
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1757-4676
EI 1757-4684
J9 EMBO MOL MED
JI EMBO Mol. Med.
PD JUL
PY 2016
VL 8
IS 7
BP 685
EP 687
DI 10.15252/emmm.201606280
PG 3
WC Medicine, Research & Experimental
SC Research & Experimental Medicine
GA DW4SC
UT WOS:000383632300001
PM 27234439
ER
PT J
AU Yoon, SL
Roh, YG
Chu, IS
Heo, J
Kim, SI
Chang, H
Kang, TH
Chung, JW
Koh, SS
Larionov, V
Leem, SH
AF Yoon, Se-Lyun
Roh, Yun-Gil
Chu, In-Sun
Heo, Jeonghoon
Kim, Seung Il
Chang, Heekyung
Kang, Tae-Hong
Chung, Jin Woong
Koh, Sang Seok
Larionov, Vladimir
Leem, Sun-Hee
TI A polymorphic minisatellite region of BORIS regulates gene expression
and its rare variants correlate with lung cancer susceptibility
SO EXPERIMENTAL AND MOLECULAR MEDICINE
LA English
DT Article
ID TRANSCRIPTION FACTORS; GASTRIC-CARCINOMA; VARIABLE NUMBER;
PROSTATE-CANCER; BREAST-CANCER; TESTIS GENE; CELLS; CTCF; ALLELES;
DEREPRESSION
AB Aberrant expression of BORIS/CTCFL (Brother of the Regulator of Imprinted Sites/CTCF-like protein) is reported in different malignancies. In this study, we characterized the entire promoter region of BORIS/CTCFL, including the CpG islands, to assess the relationship between BORIS expression and lung cancer. To simplify the construction of luciferase reporter cassettes with various-sized portions of the upstream region, genomic copies of BORIS were isolated using TAR cloning technology. We analyzed three promoter blocks: the GATA/CCAAT box, the CpG islands and the minisatellite region BORIS-MS2. Polymorphic minisatellite sequences were isolated from genomic DNA prepared from the blood of controls and cases. Of the three promoter blocks, the GATA/CCAAT box was determined to be a critical element of the core promoter, while the CpG islands and the BORIS-MS2 minisatellite region were found to act as regulators. Interestingly, the polymorphic minisatellite region BORIS-MS2 was identified as a negative regulator that repressed the expression levels of luciferase reporter cassettes less effectively in cancer cells compared with normal cells. We also examined the association between the size of BORIS-MS2 and lung cancer in a case-control study with 590 controls and 206 lung cancer cases. Rare alleles of BORIS-MS2 were associated with a statistically significantly increased risk of lung cancer (odds ratio, 2.04; 95% confidence interval, 1.02-4.08; and P=0.039). To conclude, our data provide information on the organization of the BORIS promoter region and gene regulation in normal and cancer cells. In addition, we propose that specific alleles of the BORIS-MS2 region could be used to identify the risk for lung cancer.
C1 [Yoon, Se-Lyun; Roh, Yun-Gil; Kang, Tae-Hong; Chung, Jin Woong; Koh, Sang Seok; Leem, Sun-Hee] Dong A Univ, Dept Biol Sci, 37 Nakdong Daero 550 Beon Gil, Busan 49315, South Korea.
[Chu, In-Sun] Korea Res Inst Biosci & Biotechnol, Korean Bioinformat Ctr, Daejeon, South Korea.
[Heo, Jeonghoon] Kosin Univ, Dept Mol Biol & Immunol, Coll Med, Busan, South Korea.
[Kim, Seung Il] Korea Basic Sci Inst, Drug & Dis Target Team, Daejeon, South Korea.
[Chang, Heekyung] Kosin Univ, Dept Pathol, Coll Med, Busan, South Korea.
[Larionov, Vladimir] NCI, Dev Therapeut Branch, Bethesda, MD 20892 USA.
RP Leem, SH (reprint author), Dong A Univ, Dept Biol Sci, 37 Nakdong Daero 550 Beon Gil, Busan 49315, South Korea.
EM shleem@dau.ac.kr
FU Mid-Career Researcher Program through the National Research Foundation
of Korea (NRF) - Korea government (MEST) [NRF-2013R1A2A2A04008115];
Brain Busan 21 Project; Bio-Synergy Research Project of the Ministry of
Science, ICT and Future Planning through the National Research
Foundation [NRF-2014M3A9C4066461]; Korea Basic Science Institute
research program [D35402]
FX We gratefully acknowledge the patients, families and their caregivers
for their willing participation in this project and for providing
consent for the use of their information in this study. The study design
and procedures were approved by the Committee of Bioethics at Dong-A
University (#IRB-06-10-02 & IRB-07-10-7; Busan, Korea). This research
was supported by the Mid-Career Researcher Program through the National
Research Foundation of Korea (NRF) grant (NRF-2013R1A2A2A04008115)
funded by the Korea government (MEST). This work was supported by the
Brain Busan 21 Project in 2015. SIK was supported by the Bio-Synergy
Research Project (NRF-2014M3A9C4066461) of the Ministry of Science, ICT
and Future Planning through the National Research Foundation and the
Korea Basic Science Institute research program (D35402).
NR 34
TC 1
Z9 1
U1 1
U2 1
PU NATURE PUBLISHING GROUP
PI NEW YORK
PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA
SN 1226-3613
EI 2092-6413
J9 EXP MOL MED
JI Exp. Mol. Med.
PD JUL
PY 2016
VL 48
AR e246
DI 10.1038/emm.2016.50
PG 10
WC Biochemistry & Molecular Biology; Medicine, Research & Experimental
SC Biochemistry & Molecular Biology; Research & Experimental Medicine
GA EE5LG
UT WOS:000389648400001
PM 27416782
ER
PT J
AU Klinger, SC
Hojland, A
Jain, S
Kjolby, M
Madsen, P
Svendsen, AD
Olivecrona, G
Bonifacino, JS
Nielsen, MS
AF Klinger, Stine C.
Hojland, Anne
Jain, Shweta
Kjolby, Mads
Madsen, Peder
Svendsen, Anna Dorst
Olivecrona, Gunilla
Bonifacino, Juan S.
Nielsen, Morten S.
TI Polarized trafficking of the sorting receptor SorLA in neurons and MDCK
cells
SO FEBS JOURNAL
LA English
DT Article
DE adaptor protein; AP1; cell polarity; epithelial cell; intracellular
trafficking; LR11; neurons; SorLA; sorting
ID AMYLOID PRECURSOR PROTEIN; CYTOPLASMIC DOMAIN; LIPOPROTEIN-LIPASE;
EPITHELIAL-CELLS; HIPPOCAMPAL-NEURONS; STRUCTURAL BASIS; ADIPOSE-TISSUE;
SIGNAL; BINDING; MOTIF
AB The sorting receptor SorLA is highly expressed in neurons and is also found in other polarized cells. The receptor has been reported to participate in the trafficking of several ligands, some of which are linked to human diseases, including the amyloid precursor protein, TrkB, and Lipoprotein Lipase (LpL). Despite this, only the trafficking in nonpolarized cells has been described so far. Due to the many differences between polarized and nonpolarized cells, we examined the localization and trafficking of SorLA in epithelial Madin-Darby canine kidney (MDCK) cells and rat hippocampal neurons. We show that SorLA is mainly found in sorting endosomes and on the basolateral surface of MDCK cells and in the somatodendritic domain of neurons. This polarized distribution of SorLA respectively depends on an acidic cluster and an extended version of this cluster and involves the cellular adaptor complex AP-1. Furthermore, we show that SorLA can mediate transcytosis across a tight cell layer.
C1 [Klinger, Stine C.; Hojland, Anne; Madsen, Peder; Svendsen, Anna Dorst; Nielsen, Morten S.] Aarhus Univ, Lundbeck Fdn Initiat Brain Barriers & Drug Delive, Dept Biomed, Aarhus, Denmark.
[Klinger, Stine C.; Hojland, Anne; Kjolby, Mads; Madsen, Peder; Svendsen, Anna Dorst; Nielsen, Morten S.] Aarhus Univ, MIND Ctr, Dept Biomed, Aarhus, Denmark.
[Klinger, Stine C.; Jain, Shweta; Bonifacino, Juan S.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Cell Biol & Metab Program, NIH, Bethesda, MD USA.
[Kjolby, Mads] Aarhus Univ, Danish Diabet Acad, Dept Biomed, Aarhus, Denmark.
[Olivecrona, Gunilla] Umea Univ, Physiol Chem, Dept Med Biosci, Umea, Sweden.
RP Nielsen, MS (reprint author), Ole Worms Alle 1170, DK-8000 Aarhus, Denmark.
EM mn@biomed.au.dk
OI Bonifacino, Juan S./0000-0002-5673-6370
FU Alfred Benzon Foundation; Lundbeck Foundation [R108-A10265, R93-A8846];
Lundbeck Foundation Research Initiative on Blood Brain and Drug
Delivery; Danish Diabetes Academy; Novo Nordisk Foundation; Intramural
Program of NICHD, NIH
FX We thank F. Kamakh, N. Mahler, X. Zhu, and N. Tsai for expert technical
assistance and T. Ryan and R. Fenton for kind gifts of reagents or
cells. The work was supported by the Alfred Benzon Foundation (to SCK),
The Lundbeck Foundation (project number R108-A10265 (to SCK) and project
number R93-A8846), The Lundbeck Foundation Research Initiative on Blood
Brain and Drug Delivery, The Danish Diabetes Academy, Novo Nordisk
Foundation (MK) and The Intramural Program of NICHD, NIH. The funders
had no role in study design, data collection, and interpretation, or the
decision to submit the work for publication.
NR 48
TC 0
Z9 0
U1 1
U2 1
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1742-464X
EI 1742-4658
J9 FEBS J
JI FEBS J.
PD JUL
PY 2016
VL 283
IS 13
BP 2476
EP 2493
DI 10.1111/febs.13758
PG 18
WC Biochemistry & Molecular Biology
SC Biochemistry & Molecular Biology
GA DW4GR
UT WOS:000383601600007
PM 27192064
ER
PT J
AU Boikos, SA
Pappo, AS
Killian, JK
LaQuaglia, MP
Weldon, CB
George, S
Trent, JC
von Mehren, M
Wright, JA
Schiffman, JD
Raygada, M
Pacak, K
Meltzer, PS
Miettinen, MM
Stratakis, C
Janeway, KA
Helman, LJ
AF Boikos, Sosipatros A.
Pappo, Alberto S.
Killian, J. Keith
LaQuaglia, Michael P.
Weldon, Chris B.
George, Suzanne
Trent, Jonathan C.
von Mehren, Margaret
Wright, Jennifer A.
Schiffman, Josh D.
Raygada, Margarita
Pacak, Karel
Meltzer, Paul S.
Miettinen, Markku M.
Stratakis, Constantine
Janeway, Katherine A.
Helman, Lee J.
TI Molecular Subtypes of KIT/PDGFRA Wild-Type Gastrointestinal Stromal
Tumors A Report From the National Institutes of Health Gastrointestinal
Stromal Tumor Clinic
SO JAMA ONCOLOGY
LA English
DT Article
ID SUCCINATE-DEHYDROGENASE; GERMLINE MUTATIONS; IDH2 MUTATIONS; KIT;
PDGFRA; GENES; GIST; GENETICS; IMATINIB; CANCER
AB IMPORTANCE Wild-type (WT) gastrointestinal stromal tumors (GISTs), which lack KIT and PDGFRA gene mutations, are the primary form of GIST in children and occasionally occur in adults. They respond poorly to standard targeted therapy. Better molecular and clinical characterization could improve management.
OBJECTIVE To evaluate the clinical and tumor genomic features of WT GIST.
DESIGN, SETTING, AND PARTICIPANTS Patients enrolled in an observational study at the National Institutes of Health starting in 2008 and were evaluated in a GIST clinic held once or twice yearly. Patients provided access to existing medical records and tumor specimens. Self-referred or physician-referred patients younger than 19 years with GIST or 19 years or older with known WT GIST (no mutations in KIT or PDGFRA) were recruited; 116 patients with WT GIST were enrolled, and 95 had adequate tumor specimen available. Tumors were characterized by immunohistochemical analysis (IHC) for succinate dehydrogenase (SDH) subunit B, sequencing of SDH genes, and determination of SDHC promoter methylation. Testing of germline SDH genes was offered to consenting patients and families.
MAIN OUTCOMES AND MEASURES For classification, tumors were characterized by SDHA, B, C, or D (SDHX) mutations and other genetic and epigenetic alterations, including presence of mutations in germline. Clinical characteristics were categorized.
RESULTS Wild-type GIST specimens from 95 patients (median age, 23 [range, 7-78] years; 70% female) were classified into 3 molecular subtypes: SDH-competent (n = 11), defined by detection of SDHB by IHC; and 2 types of SDH-deficient GIST (n = 84). Of SDH-deficient tumors, 63 (67%) had SDH mutations, and in 31 of 38 (82%), the SDHX mutation was also present in germline. Twenty-one (22%) SDH-deficient tumors had methylation of the SDHC promoter leading to silencing of expression. Mutations in known cancer-associated pathways were identified in 9 of 11 SDH-competent tumors. Among patients with SDH-mutant tumors, 62% were female (39 of 63), median (range) age was 23 (7-58) years, and approximately 30% presented with metastases (liver [12 of 58], peritoneal [6 of 58], lymph node [15 of 23]). SDHC-epimutant tumors mostly affected young females (20 of 21; median [range] age, 15 [8-50] years), and approximately 40% presented with metastases (liver [7 of 19], peritoneal [1 of 19], lymph node [3 of 8]). SDH-deficient tumors occurred only in the stomach and had an indolent course.
CONCLUSIONS AND RELEVANCE An observational study of WT GIST permitted the evaluation of a large number of patients with this rare disease. Three molecular subtypes with implications for prognosis and clinical management were identified.
C1 [Boikos, Sosipatros A.; Helman, Lee J.] NCI, Pediat Oncol Branch, Ctr Canc Res, 9000 Rockville Pike,Bldg 31,Room 3A11, Bethesda, MD 20892 USA.
[Boikos, Sosipatros A.] Virginia Commonwealth Univ, Massey Canc Ctr, Sarcoma & Rare Tumors Program, Div Hematol Oncol & Palliat Care, Richmond, VA 23284 USA.
[Pappo, Alberto S.] St Jude Childrens Res Hosp, Dept Oncol, 332 N Lauderdale St, Memphis, TN 38105 USA.
[Killian, J. Keith; Meltzer, Paul S.] NCI, Genet Branch, Ctr Canc Res, Bethesda, MD 20892 USA.
[LaQuaglia, Michael P.] Mem Sloan Kettering Canc Ctr, Pediat Surg Serv, 1275 York Ave, New York, NY 10021 USA.
[Weldon, Chris B.] Boston Childrens Hosp, Dept Surg, Boston, MA USA.
[George, Suzanne] Dana Farber Canc Inst, Ctr Sarcoma & Bone Oncol, Boston, MA 02115 USA.
[Trent, Jonathan C.] Univ Miami, Div Hematol Oncol, Dept Med, Sylvester Comprehens Canc Ctr, Miami, FL USA.
[von Mehren, Margaret] Fox Chase Canc Ctr, Div Hematol Oncol, 7701 Burholme Ave, Philadelphia, PA 19111 USA.
[Wright, Jennifer A.; Schiffman, Josh D.] Huntsman Canc Inst, Pediat Hematol & Oncol, Salt Lake City, UT USA.
[Raygada, Margarita] Natl Inst Child Hlth & Human Dev, Div Intramural Res, Bethesda, MD USA.
[Pacak, Karel] Natl Inst Child Hlth & Human Dev, Sect Med Neuroendocrinol, Bethesda, MD USA.
[Miettinen, Markku M.] NCI, Pathol Lab, Ctr Canc Res, Bethesda, MD 20892 USA.
[Stratakis, Constantine] NICHHD, Sect Endocrinol & Genet, Bethesda, MD 20892 USA.
[Janeway, Katherine A.] Dana Farber Boston Childrens Canc & Blood Disorde, Boston, MA USA.
RP Helman, LJ (reprint author), NCI, Pediat Oncol Branch, Ctr Canc Res, 9000 Rockville Pike,Bldg 31,Room 3A11, Bethesda, MD 20892 USA.
EM helmanl@nih.gov
FU intramural National Cancer Institute (NCI), National Institutes of
Health (NIH)
FX The study was funded by the intramural National Cancer Institute (NCI),
National Institutes of Health (NIH).
NR 33
TC 8
Z9 8
U1 0
U2 0
PU AMER MEDICAL ASSOC
PI CHICAGO
PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA
SN 2374-2445
J9 JAMA ONCOL
JI JAMA Oncol.
PD JUL
PY 2016
VL 2
IS 7
BP 922
EP 928
DI 10.1001/jamaoncol.2016.0256
PG 7
WC Oncology
SC Oncology
GA DW5RK
UT WOS:000383704300016
PM 27011036
ER
PT J
AU Ramalingam, TR
Gieseck, RL
Acciani, TH
Hart, KM
Cheever, AW
Mentink-Kane, MM
Vannella, KM
Wynn, TA
AF Ramalingam, Thirumalai R.
Gieseck, Richard L.
Acciani, Thomas H.
Hart, Kevin M.
Cheever, Allen W.
Mentink-Kane, Margaret M.
Vannella, Kevin M.
Wynn, Thomas A.
TI Enhanced protection from fibrosis and inflammation in the combined
absence of IL-13 and IFN-gamma
SO JOURNAL OF PATHOLOGY
LA English
DT Article
DE fibrosis; interleukin 13; interferon; lung; liver; hydroxyproline;
collagen; fibroblast; tumour necrosis factor
ID IDIOPATHIC PULMONARY-FIBROSIS; SCHISTOSOMA-MANSONI INFECTION; INNATE
LYMPHOID-CELLS; HEPATIC-FIBROSIS; INTERFERON-GAMMA; TGF-BETA; TNF-ALPHA;
MURINE SCHISTOSOMIASIS; MOLECULAR-MECHANISMS; PERIPORTAL FIBROSIS
AB Persistent or dysregulated IL-13 responses are key drivers of fibrosis in multiple organ systems, and this identifies this cytokine as an important therapeutic target. Nevertheless, the mechanisms by which IL-13 blockade leads to the amelioration of fibrosis remain unclear. Because IFN-gamma exhibits potent anti-fibrotic activity, and IL-4R alpha signalling antagonizes IFN-gamma effector function, compensatory increases in IFN-gamma activity following IL-13/IL-4R alpha blockade might contribute to the reduction in fibrosis. To investigate the role of IFN-gamma, we developed novel IL-13(-/-)/IFN-gamma(-/-) double cytokine-deficient mice and examined disease progression in models of type 2-driven fibrosis. As predicted, we showed that fibrosis in the lung and liver are both highly dependent on IL-13. We also observed increased IFN-gamma production and inflammatory activity in the tissues of IL-13-deficient mice. Surprisingly, however, an even greater reduction in fibrosis was observed in IL-13/IFN-gamma double deficient mice, most notably in the livers of mice chronically infected with Schistosoma mansoni. The increased protection was associated with marked decreases in Tgfb1, Mmp12, and Timp1 mRNA expression in the tissues; reduced inflammation; and decreased expression of important pro-inflammatory mediators such as TNF-alpha. Experiments conducted with neutralizing monoclonal antibodies to IL-13 and IFN-gamma validated the findings with the genetically deficient mice. Together, these studies demonstrate that the reduction in fibrosis observed when IL-13 signalling is suppressed is not dependent on increased IFN-gamma activity. Instead, by reducing compensatory increases in type 1-associated inflammation, therapeutic strategies that block IFN-gamma and IL-13 activity simultaneously can confer greater protection from progressive fibrosis than IL-13 blockade alone. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.
C1 [Ramalingam, Thirumalai R.; Gieseck, Richard L.; Acciani, Thomas H.; Hart, Kevin M.; Cheever, Allen W.; Vannella, Kevin M.; Wynn, Thomas A.] NIAID, Parasit Dis Lab, Bethesda, MD USA.
[Mentink-Kane, Margaret M.] Biomed Res Inst, Rockville, MD 20852 USA.
RP Wynn, TA (reprint author), NIAID, Immunopathogenesis Sect, Parasit Dis Lab, NIH,DHHS, 4 Mem Dr,Rm 211C, Bethesda, MD 20892 USA.
EM twynn@niaid.nih.gov
FU NIAID/NIH; NIH-NIAID [HHSN272201000005I]
FX This work was supported by the intramural program of NIAID/NIH.
Schistosome-infected mice were provided by the NIAID Schistosomiasis
Resource Center at the Biomedical Research Institute (Rockville, MD,
USA) through NIH-NIAID Contract HHSN272201000005I for distribution
through BEI Resources. This work is dedicated to the memory of Dr Allen
W Cheever, who passed away during the preparation of this paper.
NR 59
TC 2
Z9 2
U1 19
U2 19
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0022-3417
EI 1096-9896
J9 J PATHOL
JI J. Pathol.
PD JUL
PY 2016
VL 239
IS 3
BP 344
EP 354
DI 10.1002/path.4733
PG 11
WC Oncology; Pathology
SC Oncology; Pathology
GA DW4EC
UT WOS:000383594300010
PM 27125685
ER
PT J
AU Pilotto, A
Gallina, P
Copetti, M
Pilotto, A
Marcato, F
Mello, AM
Simonato, M
Logroscino, G
Padovani, A
Ferrucci, L
Panza, F
AF Pilotto, Alberto
Gallina, Pietro
Copetti, Massimiliano
Pilotto, Andrea
Marcato, Francesco
Mello, Anna M.
Simonato, Matteo
Logroscino, Giancarlo
Padovani, Alessandro
Ferrucci, Luigi
Panza, Francesco
CA Multidimensional Prognostic
TI Warfarin Treatment and All-Cause Mortality in Community-Dwelling Older
Adults with Atrial Fibrillation: A Retrospective Observational Study
SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY
LA English
DT Article
DE atrial fibrillation; all-cause mortality; warfarin; aging; frailty;
multidimensional prognostic index
ID MULTIDIMENSIONAL PROGNOSTIC INDEX; RISK STRATIFICATION SCHEMES; STROKE
PREVENTION; ANTITHROMBOTIC THERAPY; ELDERLY-PATIENTS; GERIATRIC
ASSESSMENT; PREDICTING STROKE; GENERAL-PRACTICE; ANTICOAGULATION;
MANAGEMENT
AB OBJECTIVES: To investigate the relationship between warfarin treatment and different strata of all-cause mortality risk assessed using the Multidimensional Prognostic Index (MPI) based on information collected using the Standardized Multidimensional Assessment Schedule for Adults and Aged Persons (SVaMA) in community-dwelling older adults with atrial fibrillation (AF).
DESIGN: Retrospective observational study.
SETTING: Older community-dwelling adults who underwent a SVaMA evaluation establishing accessibility to homecare services and nursing home admission from 2005 to 2013 in the Padova Health District, Italy.
PARTICIPANTS: Community-dwelling individuals with AF aged 65 and older (N = 1,827).
MEASUREMENTS: Participants were classified as being at mild (MPI-SVaMA-1), moderate (MPI-SVaMA-2), or severe (MPI-SVaMA-3) risk of mortality using the MPI-SVaMA, a validated prognostic tool based on age, sex, comorbidity, cognitive status, mobility and functional disability, pressure sore risk, and social support. The association between warfarin treatment and mortality was tested using multivariate-and propensity score-adjusted Cox regression models, controlling for age, sex, all SVaMA domains, concomitant diseases, and drug treatments.
RESULTS: Higher MPI-SVaMA scores were associated with lower rates of warfarin treatment and higher 3-year mortality. After adjustment for propensity score quintiles, warfarin treatment was significantly associated with lower 2-year mortality in individuals with MPI-SVaMA-1 (hazard ratio (HR) = 0.64, 95% confidence interval (CI) = 0.50-0.82), MPI-SVaMA-2 (HR = 0.68, 95% CI = 0.55-0.85), and MPI-SVaMA-3 (HR = 0.55, 95% CI = 0.44-0.67). Heterogeneity analyses confirmed that the effect of warfarin treatment was not different between MPI-SVaMA groups (P for heterogeneity = .48).
CONCLUSION: Community-dwelling older adults with AF benefitted from anticoagulation in terms of lower all-cause mortality over a mean follow-up of 2 years, regardless of poor health and functional condition. Although this benefit can be ascribed to the treatment, it may also reflect better overall care.
C1 [Pilotto, Alberto; Mello, Anna M.] EO Galliera Hosp Natl Relevance & High Specializa, Frailty Area, Dept Geriatr Care Orthogeriatr & Rehabil, Genoa, Italy.
[Gallina, Pietro; Marcato, Francesco; Simonato, Matteo] S Antonio Hosp, Azienda Unita Locale Socio Sanitaria Padova 16, Padua, Italy.
[Copetti, Massimiliano; Panza, Francesco] Ist Ricovero & Cura Carattere Sci Casa Sollievo S, Unit Biostat, Foggia, Italy.
[Pilotto, Andrea; Padovani, Alessandro] Univ Brescia, Neurol Clin, Brescia, Italy.
[Logroscino, Giancarlo; Panza, Francesco] Univ Bari Aldo Moro, Dept Basic Med Neurosci & Sense Organs, Neurodegenerat Dis Unit, Bari, Italy.
[Logroscino, Giancarlo] Univ Bari Aldo Moro, Dept Clin Res Neurol, Lecce, Italy.
[Ferrucci, Luigi] NIA, Baltimore, MD 21224 USA.
RP Pilotto, A (reprint author), EO Galliera Hosp, NR HS, Frailty Area, Dept Geriatr Care Orthogeriatr & Rehabil, Mura Cappuccine 14, I-16128 Genoa, Italy.
EM alberto.pilotto@galliera.it
RI LOGROSCINO, GIANCARLO/K-5148-2016; Trifiro, Gianluca/K-9744-2016;
Fontana, Andrea/J-8584-2016;
OI LOGROSCINO, GIANCARLO/0000-0003-0423-3242; Trifiro,
Gianluca/0000-0003-1147-7296; Fontana, Andrea/0000-0002-6660-5315;
Panza, Francesco/0000-0002-7220-0656; Pilotto,
Andrea/0000-0003-2029-6606
FU MPI_Age European project; Executive Agency for Health and Consumers of
the European Innovation Partnership on Active and Healthy Ageing Second
Health Programme
FX Funding for this study was provided by the MPI_Age European project,
cofunded by the Executive Agency for Health and Consumers of the
European Innovation Partnership on Active and Healthy Ageing Second
Health Programme 2008-2013.
NR 48
TC 1
Z9 1
U1 2
U2 2
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0002-8614
EI 1532-5415
J9 J AM GERIATR SOC
JI J. Am. Geriatr. Soc.
PD JUL
PY 2016
VL 64
IS 7
BP 1416
EP 1424
DI 10.1111/jgs.14221
PG 9
WC Geriatrics & Gerontology; Gerontology
SC Geriatrics & Gerontology
GA DW3NN
UT WOS:000383548400005
PM 27295351
ER
PT J
AU Wang, SM
Fan, JH
Jia, MM
Yang, Z
Zhang, YQ
Qiao, YL
Taylor, PR
AF Wang, Shao-Ming
Fan, Jin-Hu
Jia, Meng-Meng
Yang, Zhao
Zhang, Yu-Qing
Qiao, You-Lin
Taylor, Philip R.
TI Body mass index and long-term risk of death from esophageal squamous
cell carcinoma in a Chinese population
SO THORACIC CANCER
LA English
DT Article
DE Body mass index (BMI); Chinese population; esophageal squamous cell
carcinoma (ESCC); overweight; underweight
ID GASTRIC CANCERS; FOLLOW-UP; MORTALITY; OBESITY; ADENOCARCINOMA;
METAANALYSIS; NUTRITION; LINXIAN; MEN; SUPPLEMENTATION
AB Background: Studies based on Western populations have found that body mass index (BMI) is positively related to the risk of esophageal adenocarcinoma but inversely associated with esophageal squamous cell carcinoma (ESCC). Little reliable evidence exists of an association between BMI and ESCCin China, where ESCC incidence is high but BMI is low.
Methods: We evaluated the BMI-ESCC association in a population-based prospective study of 29 446 Chinese aged 40-69 with 27 years of follow-up. China-specific BMI cut-offs (underweight < 18.5, healthy >= 18.5 to <24, overweight >= 24 to <28, and obese >= 28) and quartile categories were used to define BMI subgroups. Adjusted hazard ratios (HRs) and confidence intervals (CIs) for death from ESCC by BMI subgroups were calculated using Cox proportional hazards models.
Results: During a median follow-up duration of 21.2 years (555 439 person-years), 2436 ESCC deaths were identified. BMI was protective for death from ESCC with an HR of 0.97 (95% CI 0.95-0.99) for each unit increase in BMI. Relative to healthy weight, HRs for BMI were 1.21 (95% CI 1.02-1.43) for the underweight group and 0.87 (95% CI 0.78-0.98) for the overweight. Categorical quartile analyses found people with BMIs in the Q3 and Q4 groups had 16% and 13% reductions in the risk of ESCC, respectively. Gender-specific analyses found that clear effects were evident in women only.
Conclusions: Higher BMI was associated with a reduced risk of ESCC in a Chinese population.
C1 [Wang, Shao-Ming; Fan, Jin-Hu; Jia, Meng-Meng; Yang, Zhao; Zhang, Yu-Qing; Qiao, You-Lin] Chinese Acad Med Sci, Canc Inst Hosp, 17 South Panjiayuan Lane,POB 2258, Beijing 100021, Peoples R China.
[Wang, Shao-Ming; Fan, Jin-Hu; Jia, Meng-Meng; Yang, Zhao; Zhang, Yu-Qing; Qiao, You-Lin] Peking Union Med Coll, 17 South Panjiayuan Lane,POB 2258, Beijing, Peoples R China.
[Taylor, Philip R.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.
RP Qiao, YL (reprint author), Chinese Acad Med Sci, Canc Inst Hosp, 17 South Panjiayuan Lane,POB 2258, Beijing 100021, Peoples R China.; Qiao, YL (reprint author), Peking Union Med Coll, 17 South Panjiayuan Lane,POB 2258, Beijing, Peoples R China.
EM qiaoy@cicams.ac.cn
OI Qiao, You-Lin/0000-0001-6380-0871
FU US National Cancer Institute [HHSN261201200034C]; [2013JKB01]
FX This study was funded by a US National Cancer Institute contract
(HHSN261201200034C) to the Cancer Hospital/ Institute, Chinese Academy
of Medical Sciences and a basic research grant (2013JKB01) to the Cancer
Hospital/Institute, Chinese Academy of Medical Sciences.
NR 23
TC 0
Z9 0
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1759-7706
EI 1759-7714
J9 THORAC CANCER
JI Thorac. Cancer
PD JUL
PY 2016
VL 7
IS 4
BP 387
EP 392
DI 10.1111/1759-7714.12340
PG 6
WC Oncology; Respiratory System
SC Oncology; Respiratory System
GA DW5YY
UT WOS:000383724800002
PM 27385979
ER
PT J
AU Cansu, GB
Taskiran, B
Trivellin, G
Faucz, FR
Stratakis, CA
AF Cansu, Guven Baris
Taskiran, Bengur
Trivellin, Giampaolo
Faucz, Fabio R.
Stratakis, Constantine A.
TI A novel truncating AIP mutation, p.W279*, in a familial isolated
pituitary adenoma (FIPA) kindred
SO HORMONES-INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
LA English
DT Article
DE AIP mutation; Familial isolated pituitary adenoma; Gigantism
ID GENE
AB Familial isolated pituitary adenomas (FIPA) constitute 2-3% of pituitary tumours. AIP is the most commonly mutated gene in FIPA. We herein report a novel germline mutation of the AIP gene in a family with FIPA. We present two patients, a father and his 12-year-old daughter, diagnosed clinically and using laboratory measures with acromegaly-gigantism. Both underwent transsphenoidal hypophyseal surgery for macroadenomas. We initially detected a novel heterozygous germline AIP mutation, c.836G> A (p.W279*), in the father's DNA. We then found the same mutation in his affected daughter. Pituitary adenomas associated with AIP mutations mostly present as FIPA (68%) at an early age (78% occur at <30 years old). They are often growth hormone (GH) - or prolactin - secreting macroadenomas (88%) that have already extended beyond the sella at the time of diagnosis. Acromegalic cases are resistant to somatostatin analogues and multimodal management is frequently essential to control the disease. Our patients had normalized GH/IGF-1 values soon after surgery, although enough time may not have elapsed to reach final cure. While penetrance of the disease can be as low as 10% in FIPA, especially children and young patients with somatotropinoma and prolactinoma should be surveyed for inactivating mutations or deletions in AIP. Determining the causative mutations may be of assistance in early diagnosis, treatment success, and genetic counseling.
C1 [Cansu, Guven Baris; Taskiran, Bengur] Yunus Emre State Hosp, Div Endocrinol & Metab, Uluonder Mah Salih Bozok Cad 23, TR-26190 Tepebasi, Eskisehir, Turkey.
[Trivellin, Giampaolo; Faucz, Fabio R.; Stratakis, Constantine A.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Endocrinol & Genet, NIH, Bethesda, MD 20892 USA.
RP Cansu, GB (reprint author), Yunus Emre State Hosp, Div Endocrinol & Metab, Uluonder Mah Salih Bozok Cad 23, TR-26190 Tepebasi, Eskisehir, Turkey.
EM bcansu74@hotmail.com
OI Trivellin, Giampaolo/0000-0003-2384-4153; Cansu, Guven
Baris/0000-0003-2606-1103
FU Intramural Research Program of the Eunice Kennedy Shriver National
Institute of Child Health & Human Development (NICHD), National
Institutes of Health (NIH), Bethesda, MD, USA [Z01-HD008920]
FX This study was supported by the research project Z01-HD008920 (Principal
Investigator: Dr. Constantine A. Stratakis) of the Intramural Research
Program of the Eunice Kennedy Shriver National Institute of Child Health
& Human Development (NICHD), National Institutes of Health (NIH),
Bethesda, MD, USA.
NR 12
TC 0
Z9 0
U1 0
U2 0
PU HELLENIC ENDOCRINE SOC
PI ATHENS
PA 14 ALEXANDRAS AVE, ATHENS, 106 82, GREECE
SN 1109-3099
J9 HORM-INT J ENDOCRINO
JI Horm.-Int. J. Endocrinol. Metab.
PD JUL-SEP
PY 2016
VL 15
IS 3
BP 441
EP 444
DI 10.14310/horm.2002.1692
PG 4
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA EA8JQ
UT WOS:000386881500012
PM 27838609
ER
PT J
AU Markantes, GK
Theodoropoulou, A
Armeni, AK
Vasileiou, V
Stratakis, CA
Georgopoulos, NA
AF Markantes, Georgios K.
Theodoropoulou, Anastasia
Armeni, Anastasia K.
Vasileiou, Vasiliki
Stratakis, Constantine A.
Georgopoulos, Neoklis A.
TI Cyclopes and Giants: From Homer's Odyssey to contemporary genetic
diagnosis
SO HORMONES-INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
LA English
DT Article
DE Acromegaly; Cyclops gigantism; Familial Isolated Pituitary Adenoma;
Hemianopia; Homer; Odysseus; Pituitary adenoma
ID PITUITARY-ADENOMA PREDISPOSITION; AIP GENE; MUTATIONS; GIGANTISM;
FEATURES; DISEASE; GROWTH
C1 [Markantes, Georgios K.; Theodoropoulou, Anastasia; Armeni, Anastasia K.; Georgopoulos, Neoklis A.] Univ Patras, Sch Med, Dept Obstet & Gynecol, Div Reprod Endocrinol, Patras, Greece.
[Vasileiou, Vasiliki] Alexandra Hosp, Ctr Diabet, Dept Endocrinol 1, Athens, Greece.
[Stratakis, Constantine A.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Endocrinol & Genet, NIH, Bethesda, MD USA.
RP Georgopoulos, NA (reprint author), Univ Patras, Sch Med, Div Reprod Endocrinol, Dept Obstet & Gynecol, Rion 26500, Greece.
EM neoklisg@hol.gr
NR 21
TC 0
Z9 0
U1 0
U2 0
PU HELLENIC ENDOCRINE SOC
PI ATHENS
PA 14 ALEXANDRAS AVE, ATHENS, 106 82, GREECE
SN 1109-3099
J9 HORM-INT J ENDOCRINO
JI Horm.-Int. J. Endocrinol. Metab.
PD JUL-SEP
PY 2016
VL 15
IS 3
BP 459
EP 463
DI 10.14310/horm.2002.1687
PG 5
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA EA8JQ
UT WOS:000386881500015
PM 27394709
ER
PT J
AU Ahluwalia, A
Gladwin, M
Coleman, GD
Hord, N
Howard, G
Kim-Shapiro, DB
Lajous, M
Larsen, FJ
Lefer, DJ
McClure, LA
Nolan, BT
Pluta, R
Schechter, A
Wang, CY
Ward, MH
Harman, JL
AF Ahluwalia, Amrita
Gladwin, Mark
Coleman, Gary D.
Hord, Norman
Howard, George
Kim-Shapiro, Daniel B.
Lajous, Martin
Larsen, Filip J.
Lefer, David J.
McClure, Leslie A.
Nolan, Bernard T.
Pluta, Ryszard
Schechter, Alan
Wang, Chia-Yih
Ward, Mary H.
Harman, Jane L.
TI Dietary Nitrate and the Epidemiology of Cardiovascular Disease: Report
From a National Heart, Lung, and Blood Institute Workshop
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE national registry; nitrate; nitric oxide; nutrition
ID N-NITROSO COMPOUNDS; PRESERVED EJECTION FRACTION; VIVO
ISCHEMIA-REPERFUSION; NITRIC-OXIDE SYNTHASE; GASTRIC-CANCER RISK;
INORGANIC NITRATE; VEGETABLE INTAKE; ENDOGENOUS NITROSATION;
DRINKING-WATER; IN-VIVO
C1 [Ahluwalia, Amrita] Queen Mary Univ London, William Harvey Res Inst, Barts & London Med Sch, London, England.
[Gladwin, Mark] Univ Pittsburgh, Vasc Med Inst, Pittsburgh, PA USA.
[Coleman, Gary D.] Univ Maryland, College Pk, MD 20742 USA.
[Hord, Norman] Oregon State Univ, Corvallis, OR 97331 USA.
[Howard, George] Univ Alabama Birmingham, Birmingham, AL USA.
[Kim-Shapiro, Daniel B.] Wake Forest Univ, Winston Salem, NC 27109 USA.
[Lajous, Martin] Nacl Salud Publ Mexico, Mexico, Albania.
[Larsen, Filip J.] Karolinska Inst, Stockholm, Sweden.
[Lefer, David J.] Louisiana State Univ, Hlth Sci Ctr, New Orleans, LA USA.
[McClure, Leslie A.] Drexel Univ, Dornsife Sch Publ Hlth, Philadelphia, PA 19104 USA.
[Nolan, Bernard T.] US Geol Survey, 959 Natl Ctr, Reston, VA 22092 USA.
[Pluta, Ryszard] NINDS, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
[Schechter, Alan] NIDDKD, Bethesda, MD USA.
[Wang, Chia-Yih] CDC, Natl Ctr Hlth Stat, Hyattsville, MD USA.
[Ward, Mary H.] NCI, Rockville, MD USA.
[Harman, Jane L.] NHLBI, Div Cardiovasc Sci, Bldg 10, Bethesda, MD 20892 USA.
RP Ahluwalia, A (reprint author), Queen Mary Univ London, William Harvey Res Inst, Charterhouse Sq, London EC1M 6BQ, England.
EM a.ahluwalia@qmul.ac.uk
FU National Heart, Lung, and Blood Institute
FX The workshop was convened and funded by the National Heart, Lung, and
Blood Institute.
NR 120
TC 0
Z9 0
U1 4
U2 4
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003402
DI 10.1161/JAHA.116.003402
PG 10
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800029
ER
PT J
AU Bulluck, H
Rosmini, S
Abdel-Gadir, A
White, SK
Bhuva, AN
Treibel, TA
Fontana, M
Gonzalez-Lopez, E
Reant, P
Ramlall, M
Hamarneh, A
Sirker, A
Herrey, AS
Manisty, C
Yellon, DM
Kellman, P
Moon, JC
Hausenloy, DJ
AF Bulluck, Heerajnarain
Rosmini, Stefania
Abdel-Gadir, Amna
White, Steven K.
Bhuva, Anish N.
Treibel, Thomas A.
Fontana, Marianna
Gonzalez-Lopez, Esther
Reant, Patricia
Ramlall, Manish
Hamarneh, Ashraf
Sirker, Alex
Herrey, Anna S.
Manisty, Charlotte
Yellon, Derek M.
Kellman, Peter
Moon, James C.
Hausenloy, Derek J.
TI Automated Extracellular Volume Fraction Mapping Provides Insights Into
the Pathophysiology of Left Ventricular Remodeling Post-Reperfused
ST-Elevation Myocardial Infarction
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE extracellular volume fraction; left ventricular remodeling; ST-segment
elevation myocardial infarction; T1 mapping; T2 mapping
ID CARDIOVASCULAR MAGNETIC-RESONANCE; MICROVASCULAR OBSTRUCTION; T2
QUANTIFICATION; HEART-ASSOCIATION; TASK-FORCE; SALVAGE; CMR; MORTALITY;
EDEMA; SIZE
AB Background-Whether the remote myocardium of reperfused ST-segment elevation myocardial infarction (STEMI) patients plays a part in adverse left ventricular (LV) remodeling remains unclear. We aimed to use automated extracellular volume fraction (ECV) mapping to investigate whether changes in the ECV of the remote (ECVRemote) and infarcted myocardium (ECVInfarct) impacted LV remodeling.
Methods and Results-Forty-eight of 50 prospectively recruited reperfused STEMI patients completed a cardiovascular magnetic resonance at 4 +/- 2 days and 40 had a follow-up scan at 5 +/- 2 months. Twenty healthy volunteers served as controls. Mean segmental values for native T1, T2, and ECV were obtained. Adverse LV remodeling was defined as >= 20% increase in LV end-diastolic volume. ECVRemote was higher on the acute scan when compared to control (27.9 +/- 2.1% vs 26.4 +/- 2.1%; P=0.01). Eight patients developed adverse LV remodeling and had higher ECVRemote acutely (29.5 +/- 1.4% vs 27.4 +/- 2.0%; P=0.01) and remained higher at follow-up (28.6 +/- 1.5% vs 26.6 +/- 2.1%; P=0.02) compared to those without. Patients with a higher ECVRemote and a lower myocardial salvage index (MSI) acutely were significantly associated with adverse LV remodeling, independent of T1(Remote), T1(Core) and microvascular obstruction, whereas a higher ECVInfarct was significantly associated with worse wall motion recovery.
Conclusions-ECVRemote was increased acutely in reperfused STEMI patients. Those with adverse LV remodeling had higher ECVRemote acutely, and this remained higher at follow-up than those without adverse LV remodeling. A higher ECVRemote and a lower MSI acutely were significantly associated with adverse LV remodeling whereas segments with higher ECVInfarct were less likely to recover wall motion.
C1 [Bulluck, Heerajnarain; White, Steven K.; Ramlall, Manish; Hamarneh, Ashraf; Yellon, Derek M.; Hausenloy, Derek J.] UCL, Inst Cardiovasc Sci, Hatter Cardiovasc Inst, London, England.
[Bulluck, Heerajnarain; White, Steven K.; Ramlall, Manish; Hamarneh, Ashraf; Sirker, Alex; Yellon, Derek M.; Moon, James C.; Hausenloy, Derek J.] UCL, Hosp Biomed Res Ctr, Natl Inst Hlth Res, London, England.
[Bulluck, Heerajnarain; Rosmini, Stefania; Abdel-Gadir, Amna; White, Steven K.; Bhuva, Anish N.; Treibel, Thomas A.; Fontana, Marianna; Ramlall, Manish; Hamarneh, Ashraf; Sirker, Alex; Herrey, Anna S.; Manisty, Charlotte; Moon, James C.; Hausenloy, Derek J.] St Bartholomews Hosp, Barts Heart Ctr, London, England.
[Gonzalez-Lopez, Esther] Hosp Univ Puerta de Hierro Majadahonda, Madrid, Spain.
[Reant, Patricia] Univ Bordeaux, CHU Bordeaux, Bordeaux, France.
[Kellman, Peter] NHLBI, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Hausenloy, Derek J.] Duke Natl Univ Singapore, Cardiovasc & Metab Disorders Program, Singapore, Singapore.
[Hausenloy, Derek J.] Natl Heart Ctr Singapore, Natl Heart Res Inst Singapore, Singapore, Singapore.
RP Hausenloy, DJ (reprint author), Duke NUS Grad Med Sch Singapore, Cardiovasc & Metab Dis Program, 8 Coll Rd, Singapore 169857, Singapore.
EM derek.hausenloy@duke-nus.edu.sg
OI Hamarneh, Ashraf/0000-0002-9489-4476; Bulluck,
Heerajnarain/0000-0002-1985-1783
FU British Heart Foundation [FS/10/039/28270]; Rosetrees Trust; National
Institute for Health Research University College London Hospitals
Biomedical Research Center
FX This work was supported by the British Heart Foundation
(FS/10/039/28270), the Rosetrees Trust, and the National Institute for
Health Research University College London Hospitals Biomedical Research
Center
NR 34
TC 2
Z9 2
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003555
DI 10.1161/JAHA.116.003555
PG 11
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800045
ER
PT J
AU Dember, LM
Imrey, PB
Duess, MA
Hamburg, NM
Larive, B
Radeva, M
Himmelfarb, J
Kraiss, LW
Kusek, JW
Roy-Chaudhury, P
Terry, CM
Vazquez, MA
Vongpatanasin, W
Beck, GJ
Vita, JA
AF Dember, Laura M.
Imrey, Peter B.
Duess, Mai-Ann
Hamburg, Naomi M.
Larive, Brett
Radeva, Milena
Himmelfarb, Jonathan
Kraiss, Larry W.
Kusek, John W.
Roy-Chaudhury, Prabir
Terry, Christi M.
Vazquez, Miguel A.
Vongpatanasin, Wanpen
Beck, Gerald J.
Vita, Joseph A.
CA Hemodialysis Fistula Maturation St
TI Vascular Function at Baseline in the Hemodialysis Fistula Maturation
Study
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE chronic kidney disease; end-stage renal disease; flow-mediated dilation;
nitroglycerin-mediated dilation; pulse wave velocity; vascular function;
venous occlusion plethysmography
ID FLOW-MEDIATED DILATION; STAGE RENAL-DISEASE; PULSE-WAVE VELOCITY;
DEPENDENT DIABETES-MELLITUS; ARTERIAL STIFFNESS;
PROGNOSTIC-SIGNIFICANCE; CARDIOVASCULAR RESEARCH; ARTERIOVENOUS-FISTULA;
ENDOTHELIAL FUNCTION; AORTIC STIFFNESS
AB Background-End-stage renal disease is accompanied by functional and structural vascular abnormalities. The objective of this study was to characterize vascular function in a large cohort of patients with end-stage renal disease, using noninvasive physiological measurements, and to correlate function with demographic and clinical factors.
Methods and Results-We analyzed cross-sectional baseline data from the Hemodialysis Fistula Maturation Study, a multicenter prospective observational cohort study of 602 patients with end-stage renal disease from 7 centers. Brachial artery flow-and nitroglycerin-mediated dilation, carotid-femoral and -radial pulse wave velocity, and venous occlusion plethysmography were performed prior to arteriovenous fistula creation. Relationships of these vascular function measures with demographic, clinical, and laboratory factors were evaluated using linear mixed-effects models. Arterial function, as assessed by flow-and nitroglycerin-mediated dilation and carotid-femoral pulse wave velocity, worsened with increasing age and diabetes mellitus. Venous capacitance decreased with diabetes mellitus but not with age. Flow-mediated dilation was higher among patients undergoing maintenance dialysis than for those at predialysis, and a U-shaped relationship between serum phosphorus concentration and flow-mediated dilation was evident. Partial correlations among different measures of vascular function, adjusting for demographic factors, diabetes mellitus, and clinical center, were modest or essentially nonexistent.
Conclusions-Multiple demographic and clinical factors were associated with the functions of vessels of different sizes and types in this large cohort of patients with end-stage renal disease. Low correlations between the different measures, controlling for demographic factors, diabetes mellitus, and center, indicated that these different types of vascular function otherwise vary heterogeneously across patients.
C1 [Dember, Laura M.] Univ Penn, Perelman Sch Med, 920 Blockley Hall,423 Guardian Dr, Philadelphia, PA 19104 USA.
[Imrey, Peter B.; Larive, Brett; Radeva, Milena; Beck, Gerald J.] Case Western Reserve Univ, Dept Quantitat Hlth Sci, Cleveland Clin, Lerner Coll Med, Cleveland, OH 44106 USA.
[Duess, Mai-Ann; Hamburg, Naomi M.; Vita, Joseph A.] Boston Univ, Sch Med, Boston, MA 02118 USA.
[Himmelfarb, Jonathan] Univ Washington, Seattle, WA 98195 USA.
[Kraiss, Larry W.; Terry, Christi M.] Univ Utah, Salt Lake City, UT USA.
[Kusek, John W.] NIDDKD, Bethesda, MD USA.
[Roy-Chaudhury, Prabir] Univ Arizona, Tucson, AZ USA.
[Vazquez, Miguel A.; Vongpatanasin, Wanpen] Univ Texas Southwestern, Dallas, TX USA.
RP Dember, LM (reprint author), Univ Penn, Renal Electrolyte & Hypertens Div, Ctr Clin Epidemiol & Biostat, Perelman Sch Med, 920 Blockley Hall,423 Guardian Dr, Philadelphia, PA 19104 USA.
EM ldember@upenn.edu
FU National Institute of Diabetes and Digestive and Kidney Diseases
[U01DK066597, U01DK082179, U01DK082189, U01DK082218, U01DK082222,
U01DK082232, U01DK082236, U01DK082240]
FX The Hemodialysis Fistula Maturation Study was funded by grants
U01DK066597, U01DK082179, U01DK082189, U01DK082218, U01DK082222,
U01DK082232, U01DK082236, and U01DK082240 from the National Institute of
Diabetes and Digestive and Kidney Diseases.
NR 33
TC 1
Z9 1
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003227
DI 10.1161/JAHA.116.003227
PG 26
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800019
ER
PT J
AU Haring, R
Enserro, D
Xanthakis, V
Mitchell, GF
Benjamin, EJ
Hamburg, NM
Sullivan, L
Nauck, M
Wallaschofski, H
Vasan, RS
AF Haring, Robin
Enserro, Danielle
Xanthakis, Vanessa
Mitchell, Gary F.
Benjamin, Emelia J.
Hamburg, Naomi M.
Sullivan, Lisa
Nauck, Matthias
Wallaschofski, Henri
Vasan, Ramachandran S.
TI Plasma Fibroblast Growth Factor 23: Clinical Correlates and Association
With Cardiovascular Disease and Mortality in the Framingham Heart Study
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE cardiovascular disease risk factors; epidemiology; metabolism; mineral
ID CHRONIC KIDNEY-DISEASE; CORONARY-ARTERY-DISEASE; MINERAL METABOLISM;
PARATHYROID-HORMONE; ELDERLY-MEN; COMMUNITY; EVENTS; PHOSPHORUS; FGF-23;
RISK
AB Background-Fibroblast growth factor 23 (FGF23) is emerging as a novel biomarker of bone metabolism, chronic kidney disease, and cardiovascular disease (CVD). However, its clinical correlates and potential predictive role in a community-based setting are incompletely understood.
Methods and Results-We evaluated participants of the Framingham Heart Study (seventh examination cycle of the Offspring cohort plus second examination cycle of the multiethnic Omni cohort) to identify clinical correlates of plasma FGF23 (N=3236) and examine its cross-sectional association with vascular function (N=2209), and longitudinal association with 10-year incidence of CVD (N=2823), and all-cause mortality (N=3223). Circulating FGF23 concentrations were positively related to African-American and Asian ethnicity, waist circumference, current smoking, serum glucose, history of CVD, and antihypertensive medication use; and negatively related to male sex, hormone replacement therapy, and estimated glomerular filtration rate. Multivariable-adjusted cross-sectional analyses showed no consistent association of FGF23 with vascular function measures. During a median follow-up time of 10.8 years, 347 incident CVD events and 412 deaths occurred. Multivariable-adjusted Cox regression models revealed a positive association of FGF23 with all-cause mortality (hazard ratio [HR] per SD increase, 1.31; 95% CI, 1.20-1.42), but not with incident CVD (HR per SD increase, 1.05; 95% CI, 0.94-1.17).
Conclusions-In our large, community-based sample, FGF23 was associated with mortality risk, but not with vascular function or incident CVD.
C1 [Haring, Robin; Xanthakis, Vanessa; Benjamin, Emelia J.; Vasan, Ramachandran S.] Boston Univ, Sch Med, Prevent Med & Epidemiol Sect, Boston, MA 02118 USA.
[Benjamin, Emelia J.; Hamburg, Naomi M.; Vasan, Ramachandran S.] Boston Univ, Sch Med, Evans Dept Med, Cardiol Sect, Boston, MA 02118 USA.
[Haring, Robin] European Univ Appl Sci, Fac Appl Publ Hlth, Rostock, Germany.
[Enserro, Danielle; Sullivan, Lisa] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02215 USA.
[Benjamin, Emelia J.; Vasan, Ramachandran S.] Boston Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02215 USA.
[Xanthakis, Vanessa; Benjamin, Emelia J.; Vasan, Ramachandran S.] NHLBI, Framingham, MA USA.
[Xanthakis, Vanessa; Benjamin, Emelia J.; Vasan, Ramachandran S.] Boston Univ, Framingham Heart Study, Framingham, MA USA.
[Mitchell, Gary F.] Cardiovasc Engn Inc, Norwood, MA USA.
[Nauck, Matthias; Wallaschofski, Henri] Univ Med Greifswald, Inst Clin Chem & Lab Med, Ferdinand Sauerbruch Str, D-17475 Greifswald, Germany.
[Nauck, Matthias] German Ctr Cardiovasc Res DZHK eV Partner Site Gr, Greifswald, Germany.
RP Haring, R (reprint author), Univ Med Greifswald, Inst Clin Chem & Lab Med, Ferdinand Sauerbruch Str, D-17475 Greifswald, Germany.
EM robin.haring@uni-greifswald.de
FU Framingham Heart Study [HHSN268201500001I, 1R01HL60040, 1RO1HL70100,
N01-HC-25195]
FX The Framingham Heart Study is funded National Heart, Lung, and Blood
Institute (N01-HC-25195, HHSN268201500001I, 1R01HL60040, 1RO1HL70100).
NR 42
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U1 3
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003486
DI 10.1161/JAHA.116.003486
PG 14
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800038
ER
PT J
AU Kaess
AF Kaess
TI Circulating Brain-Derived Neurotrophic Factor Concentrations and the
Risk of Cardiovascular Disease in the Community (vol 4, e001544, 2015)
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Correction
C1 NHLBI, Framingham Heart Study, Framingham, MA USA.
NR 1
TC 0
Z9 0
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e002098
DI 10.1161/JAHA.116.002098
PG 1
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800005
ER
PT J
AU Kaess
AF Kaess
TI Relations of Central Hemodynamics and Aortic Stiffness with Left
Ventricular Structure and Function: The Framingham Heart Study (vol 5,
e002693, 2016)
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Correction
C1 NHLBI, Framingham Heart Study, Framingham, MA USA.
NR 1
TC 0
Z9 0
U1 1
U2 1
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e002100
DI 10.1161/JAHA.116.002100
PG 1
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800006
ER
PT J
AU Lin, GM
Redline, S
Klein, R
Colangelo, LA
Cotch, MF
Wong, TY
Klein, BEK
Patel, SR
Shea, SJ
Liu, K
AF Lin, Gen-Min
Redline, Susan
Klein, Ronald
Colangelo, Laura A.
Cotch, Mary Frances
Wong, Tien Y.
Klein, Barbara E. K.
Patel, Sanjay R.
Shea, Steven J.
Liu, Kiang
TI Sex-Specific Association of Obstructive Sleep Apnea With Retinal
Microvascular Signs: The Multi-Ethnic Study of Atherosclerosis
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE apnea-hypopnea index; epidemiology; microvascular dysfunction;
obstructive sleep apnea; retinal vascular calibers; retinopathy;
sex-specific
ID CARDIOVASCULAR-DISEASE; DIABETIC-RETINOPATHY; VASCULAR CALIBER; HEART
HEALTH; RISK-FACTORS; RHO KINASE; ENDOTHELIN-1; ARTERIOLES;
ABNORMALITIES; EPIDEMIOLOGY
AB Background-Obstructive sleep apnea (OSA) is a common condition affecting more men than women. The relationship of OSA with microvascular disease is unclear, complicated by possible sex difference. Assessment of the relationship of OSA with retinal microvascular signs in men and women may provide insights into such a relationship.
Methods and Results-We examined the sex-specific cross-sectional association of OSA severity with retinal vascular calibers in 1808 participants, and with specific retinopathy signs in 1831 participants from a sample of 2060 participants aged 54 to 93 years who underwent successful polysomnography in the Multi-Ethnic Study of Atherosclerosis, 2010-2012. OSA severity was defined by the apnea-hypopnea index (events/h) as none (<5), mild (5-14.9), moderate (15-29.9), and severe (>= 30). As compared to no OSA, moderate/severe OSA in men was associated with retinal arteriolar narrowing (odds ratio [OR] and 95% CI for the narrowest quartile: 1.65 [1.00-2.71]) and retinal venular widening (1.80 [1.07-3.04] for the widest quartile), but not in women (odds ratio: 1.10 [0.671.81] and 0.91 [0.58-1.43], respectively) after adjusting for age, race/ethnicity, body mass index, pack-years of cigarette smoking, alcohol intake, hypertension duration, diabetes mellitus duration, HbA1c levels, lipid profile, micro-/macroalbuminuria, estimated glomerular filtration rate, beta-blockers use, antihypertensive therapy, and lipid-lowering therapy. In contrast, severe OSA was associated with retinal microaneurysms in women, but not in men (odds ratio: 3.22 [1.16- 8.97] and 0.59 [0.27-1.30], respectively).
Conclusions-The associations of OSA severity with retinal microvascular signs may differ by sex. Whether these findings were related to sex differences in OSA exposure needs further investigation.
C1 [Lin, Gen-Min; Colangelo, Laura A.; Liu, Kiang] Northwestern Univ, Dept Prevent Med, Chicago, IL 60611 USA.
[Lin, Gen-Min] Hualien Armed Forces Gen Hosp, Dept Med, Hualien, Taiwan.
[Lin, Gen-Min] Natl Def Med Ctr, Tri Serv Gen Hosp, Taipei, Taiwan.
[Redline, Susan] Harvard Med Sch, Dept Med, Boston, MA USA.
[Redline, Susan] Brigham & Womens Hosp, 75 Francis St, Boston, MA 02115 USA.
[Redline, Susan] Beth Israel Deaconess Med Ctr, Boston, MA 02215 USA.
[Klein, Ronald; Klein, Barbara E. K.] Univ Wisconsin, Sch Med & Publ Hlth, Dept Ophthalmol & Visual Sci, Madison, WI USA.
[Cotch, Mary Frances] NEI, Div Epidemiol & Clin Applicat, NIH Intramural Res Program, NIH, Bethesda, MD 20892 USA.
[Wong, Tien Y.] Natl Univ Singapore, Singapore, Singapore.
[Wong, Tien Y.] Natl Univ Singapore, Singapore Eye Res Inst, Singapore Natl Eye Ctr, Duke NUS Grad Med Sch, Singapore, Singapore.
[Patel, Sanjay R.] Univ Pittsburgh, Dept Med, Pittsburgh, PA 15260 USA.
[Shea, Steven J.] Columbia Univ, Dept Med, New York, NY USA.
[Shea, Steven J.] Columbia Univ, Dept Epidemiol, New York, NY USA.
RP Lin, GM (reprint author), Northwestern Univ, Dept Prevent Med, Feinberg Sch Med, 680 North Lake Shore Dr,Suite 1400, Chicago, IL 60611 USA.
EM gen-min.lin@northwestern.edu
OI Patel, Sanjay/0000-0002-9142-5172
FU National Heart, Lung, and Blood Institute at the National Institutes of
Health [N01-HC-95159, N01-HC-95160, N01-HC-95161, N01-HC-95162,
N01-HC-95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167,
N01-HC-95168, N01-HC-95169]; National Center for Research Resources
[UL1-TR-000040, UL1-RR-025005, R01HL098433, R21 HL121348, T32-HL069764];
Intramural Research Program at the National Eye Institute, National
Institutes of Health [ZIAEY000403]; Ministry of Defense, Taiwan
FX This research was supported by contracts N01-HC-95159 through
N01-HC-95169 from the National Heart, Lung, and Blood Institute at the
National Institutes of Health, by grants UL1-TR-000040, UL1-RR-025005
from the National Center for Research Resources, R01HL098433 (MESA
Sleep, R21 HL121348) and T32-HL069764. Support for the visit 5 retinal
component was provided by the Intramural Research Program at the
National Eye Institute, National Institutes of Health (ZIAEY000403). A
full list of participating MESA investigators and institutions can be
found at http://www.mesa-nhlbi.org. Lin was supported by a scholarship
grant from the Ministry of Defense, Taiwan.
NR 47
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PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003598
DI 10.1161/JAHA.116.003598
PG 10
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800046
ER
PT J
AU Menard, MT
Farber, A
Assmann, SF
Choudhry, NK
Conte, MS
Creager, MA
Dake, MD
Jaff, MR
Kaufman, JA
Powell, RJ
Reid, DM
Siami, FS
Sopko, G
White, CJ
Rosenfield, K
AF Menard, Matthew T.
Farber, Alik
Assmann, Susan F.
Choudhry, Niteesh K.
Conte, Michael S.
Creager, Mark A.
Dake, Michael D.
Jaff, Michael R.
Kaufman, John A.
Powell, Richard J.
Reid, Diane M.
Siami, Flora Sandra
Sopko, George
White, Christopher J.
Rosenfield, Kenneth
TI Design and Rationale of the Best Endovascular Versus Best Surgical
Therapy for Patients With Critical Limb Ischemia (BEST-CLI) Trial
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE atherosclerosis; cost-effectiveness; critical limb ischemia;
endovascular; outcome; quality; stent treatment; surgery
ID PERIPHERAL ARTERIAL-DISEASE; COST-EFFECTIVENESS ANALYSIS; LEG BASIL
TRIAL; LOWER-EXTREMITY; THREATENING ISCHEMIA; AMPUTATION-FREE;
SAPHENOUS-VEIN; TASK-FORCE; BYPASS; ANGIOPLASTY
AB Background-Critical limb ischemia (CLI) is increasing in prevalence, and remains a significant source of mortality and limb loss. The decision to recommend surgical or endovascular revascularization for patients who are candidates for both varies significantly among providers and is driven more by individual preference than scientific evidence.
Methods and Results-The Best Endovascular Versus Best Surgical Therapy for Patients With Critical Limb Ischemia (BEST-CLI) Trial is a prospective, randomized, multidisciplinary, controlled, superiority trial designed to compare treatment efficacy, functional outcomes, quality of life, and cost in patients undergoing best endovascular or best open surgical revascularization. Approximately 140 clinical sites in the United States and Canada will enroll 2100 patients with CLI who are candidates for both treatment options. A pragmatic trial design requires consensus on patient eligibility by at least 2 investigators, but leaves the choice of specific procedural strategy within the assigned revascularization approach to the individual treating investigator. Patients with suitable single-segment of saphenous vein available for potential bypass will be randomized within Cohort 1 (n=1620), while patients without will be randomized within Cohort 2 (n= 480). The primary efficacy end point of the trial is Major Adverse Limb Event-Free Survival. Key secondary end points include Re-intervention and Amputation-Free-Survival and Amputation Free-Survival.
Conclusions-The BEST-CLI trial is the first randomized controlled trial comparing endovascular therapy to open surgical bypass in patients with CLI to be carried out in North America. This landmark comparative effectiveness trial aims to provide Level I data to clarify the appropriate role for both treatment strategies and help define an evidence-based standard of care for this challenging patient population.
C1 [Menard, Matthew T.] Brigham & Womens Hosp, Div Vasc & Endovasc Surg, 75 Francis St, Boston, MA 02115 USA.
[Choudhry, Niteesh K.] Brigham & Womens Hosp, Div Pharmacoepidemiol & Pharmacoecon, 75 Francis St, Boston, MA 02115 USA.
[Farber, Alik] Boston Med Ctr, Div Vasc & Endovasc Surg, Boston, MA USA.
[Assmann, Susan F.; Siami, Flora Sandra] New England Res Inst, 9 Galen St, Watertown, MA 02172 USA.
[Conte, Michael S.] Univ Calif San Francisco, Med Ctr, Div Vasc & Endovasc Surg, San Francisco, CA USA.
[Creager, Mark A.] Dartmouth Hitchcock Med Ctr, Dartmouth Hitchcock Heart & Vasc Ctr, Lebanon, NH 03766 USA.
[Powell, Richard J.] Dartmouth Hitchcock Med Ctr, Div Vasc Surg, Lebanon, NH 03766 USA.
[Dake, Michael D.] Stanford Hosp & Clin, Dept Cardiothorac Surg, Stanford, CA USA.
[Jaff, Michael R.] Massachusetts Gen Hosp, Fireman Vasc Ctr, Boston, MA 02114 USA.
[Rosenfield, Kenneth] Massachusetts Gen Hosp, Div Cardiol, Boston, MA 02114 USA.
[Kaufman, John A.] Dotter Intervent Inst OHSU, Portland, OR USA.
[Reid, Diane M.; Sopko, George] NHLBI, Bldg 10, Bethesda, MD 20892 USA.
[White, Christopher J.] Ochsner Med Ctr, Dept Cardiol, New Orleans, LA USA.
RP Menard, MT (reprint author), Brigham & Womens Hosp, Dept Vasc Surg, 75 Francis St, Boston, MA 02115 USA.
EM mmenard@partners.org
FU National Heart, Lung, and Blood Institute [U01HL107407, U01HL107352,
U01HL115662]
FX This trial is funded by National Heart, Lung, and Blood Institute
through grant numbers U01HL107407, U01HL107352, and U01HL115662.
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U1 2
U2 2
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003219
DI 10.1161/JAHA.116.003219
PG 21
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800018
ER
PT J
AU Sandfort, V
Lai, SH
Ahlman, MA
Mallek, M
Liu, ST
Sibley, CT
Turkbey, EB
Lima, JAC
Bluemke, DA
AF Sandfort, Veit
Lai, Shenghan
Ahlman, Mark A.
Mallek, Marissa
Liu, Songtao
Sibley, Christopher T.
Turkbey, Evrim B.
Lima, Joao A. C.
Bluemke, David A.
TI Obesity Is Associated With Progression of Atherosclerosis During Statin
Treatment
SO JOURNAL OF THE AMERICAN HEART ASSOCIATION
LA English
DT Article
DE carotid artery; carotid magnetic resonance imaging; obesity
ID LIPID-LOWERING THERAPY; RANDOMIZED CONTROLLED-TRIAL;
CORONARY-HEART-DISEASE; C-REACTIVE PROTEIN; CAROTID ATHEROSCLEROSIS;
PLAQUE PROGRESSION; RISK; EVENTS; MRI; PREDICTION
AB Background-This study aimed to determine the relationship of statin therapy and cardiovascular risk factors to changes in atherosclerosis in the carotid artery.
Methods and Results-Carotid magnetic resonance imaging was used to evaluate 106 hyperlipidemic participants at baseline and after 12 months of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (statin) treatment. Multivariable logistic regression was used to determine factors associated with progression (change in carotid wall volume >0) or regression (change <= 0) of carotid atherosclerosis. Computed tomography coronary calcium scores were obtained at baseline for all participants. The median age was 65 years (interquartile range 60-69 years), and 63% of the participants were male. Body mass index >30, elevated C-reactive protein, and hypertension were associated with increased carotid wall volume (obesity: odds ratio for progression 4.6, 95% CI 1.8-12.4, P<0.01; C-reactive protein: odds ratio for progression 2.56, 95% CI 1.17-5.73, P=0.02; hypertension: odds ratio 2.4, 95% CI 1.1-5.3, P<0.05). Higher statin dose was associated with regression of carotid wall volume (P<0.05). In multivariable analysis, obesity remained associated with progression (P<0.01), whereas statin use remained associated with regression (P<0.05). Change in atheroma volume in obese participants was +4.8% versus -4.2% in nonobese participants (P<0.05) despite greater low-density lipoprotein cholesterol reduction in obese participants.
Conclusions-In a population with hyperlipidemia, obese patients showed atheroma progression despite optimized statin therapy.
C1 [Sandfort, Veit; Ahlman, Mark A.; Mallek, Marissa; Liu, Songtao; Sibley, Christopher T.; Turkbey, Evrim B.] Natl Inst Hlth Clin Ctr, Dept Radiol & Imaging Sci, Bethesda, MD USA.
[Lai, Shenghan; Lima, Joao A. C.] Johns Hopkins Sch Med, Baltimore, MD USA.
RP Bluemke, DA (reprint author), NIH, Radiol & Imaging Sci, CC, 9000 Rockville Pike,10 Ctr Dr,Bld 10,Room 1C356, Bethesda, MD 20892 USA.
EM bluemked@cc.nih.gov
FU NIH intramural research program
FX Funding was provided by the NIH intramural research program.
NR 31
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U1 3
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-9980
J9 J AM HEART ASSOC
JI J. Am. Heart Assoc.
PD JUL
PY 2016
VL 5
IS 7
AR e003621
DI 10.1161/JAHA.116.003621
PG 13
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA EA6CT
UT WOS:000386713800051
ER
PT J
AU Akrami, K
Sweeney, DA
Malhotra, A
AF Akrami, Kevan
Sweeney, Daniel A.
Malhotra, Atul
TI Antibiotic stewardship in the intensive care unit: tools for
de-escalation from the American Thoracic Society Meeting 2016
SO JOURNAL OF THORACIC DISEASE
LA English
DT Editorial Material
ID RANDOMIZED CONTROLLED-TRIAL; ANTIMICROBIAL STEWARDSHIP; PNEUMONIA;
PROCALCITONIN
C1 [Akrami, Kevan] Univ Calif San Diego, Dept Infect Dis, San Diego, CA 92103 USA.
[Akrami, Kevan] NIH, Crit Care Med, Bldg 10, Bethesda, MD 20892 USA.
[Sweeney, Daniel A.; Malhotra, Atul] Univ Calif San Diego, Dept Pulm Crit Care & Sleep Med, San Diego, CA 92103 USA.
RP Malhotra, A (reprint author), Univ Calif San Diego, Div Pulm Crit Care & Sleep, San Diego, CA 92103 USA.
EM amalhotra@ucsd.edu
FU NHLBI NIH HHS [K24 HL132105, R01 HL081823, R01 HL085188, R01 HL119201,
R21 HL121794]
NR 12
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U1 1
U2 1
PU PIONEER BIOSCIENCE PUBL CO
PI HONG KONG
PA 9A GOLD SHINE TOWER, 346-348 QUEEN'S RD CENTRAL, SHEUNG WAN, HONG KONG,
00000, PEOPLES R CHINA
SN 2072-1439
EI 2077-6624
J9 J THORAC DIS
JI J. Thorac. Dis.
PD JUL
PY 2016
VL 8
SU 7
BP S533
EP S535
DI 10.21037/jtd.2016.07.28
PG 3
WC Respiratory System
SC Respiratory System
GA DY9FK
UT WOS:000385438900003
PM 27606085
ER
PT J
AU Noinaj, N
Mayclin, S
Stanley, AM
Jao, CC
Buchanan, SK
AF Noinaj, Nicholas
Mayclin, Stephen
Stanley, Ann M.
Jao, Christine C.
Buchanan, Susan K.
TI From Constructs to Crystals - Towards Structure Determination of
beta-barrel Outer Membrane Proteins
SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
LA English
DT Article
DE Chemistry; Issue 113; beta-barrel; membrane protein; protein
crystallization; structural biology; membranes; protein refolding;
protein purification
ID HIGH-THROUGHPUT CRYSTALLIZATION; LIGATION-INDEPENDENT CLONING; ANGSTROM
RESOLUTION; ESCHERICHIA-COLI; FIMD USHER; RECEPTOR; TRANSPORTER;
BIOGENESIS; SECRETION; NEISSERIA
AB Membrane proteins serve important functions in cells such as nutrient transport, motility, signaling, survival and virulence, yet constitute only similar to 1% percent of known structures. There are two types of membrane proteins, alpha-helical and beta-barrel. While alpha-helical membrane proteins can be found in nearly all cellular membranes, beta-barrel membrane proteins can only be found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria. One common bottleneck in structural studies of membrane proteins in general is getting enough pure sample for analysis. In hopes of assisting those interested in solving the structure of their favorite beta-barrel outer membrane protein (OMP), general protocols are presented for the production of target beta-barrel OMPs at levels useful for structure determination by either X-ray crystallography and/or NMR spectroscopy. Here, we outline construct design for both native expression and for expression into inclusion bodies, purification using an affinity tag, and crystallization using detergent screening, bicelle, and lipidic cubic phase techniques. These protocols have been tested and found to work for most OMPs from Gram-negative bacteria; however, there are some targets, particularly for mitochondria and chloroplasts that may require other methods for expression and purification. As such, the methods here should be applicable for most projects that involve OMPs from Gram-negative bacteria, yet the expression levels and amount of purified sample will vary depending on the target OMP.
C1 [Noinaj, Nicholas] Purdue Univ, Dept Biol Sci, Markey Ctr Struct Biol, W Lafayette, IN 47907 USA.
[Mayclin, Stephen; Stanley, Ann M.; Jao, Christine C.; Buchanan, Susan K.] NIDDK, NIH, Bethesda, MD USA.
[Stanley, Ann M.; Jao, Christine C.] NIGMS, NIH, Bethesda, MD USA.
RP Noinaj, N (reprint author), Purdue Univ, Dept Biol Sci, Markey Ctr Struct Biol, W Lafayette, IN 47907 USA.
EM nnoinaj@purdue.edu
FU National Institute of Diabetes and Digestive and Kidney Diseases;
Intramural Research Program at the National Institutes of Health;
National Institute of General Medical Sciences; National Institute of
Allergy and Infectious Diseases [1K22AI113078-01]; Department of
Biological Sciences at Purdue University
FX We would like to thank Herve Celia of the CNRS for providing the UV
images and Chris Dettmar and Garth Simpson in the Department of
Chemistry at Purdue University for providing the SONICC images. We would
like to acknowledge funding from the National Institute of Diabetes and
Digestive and Kidney Diseases and the Intramural Research Program at the
National Institutes of Health. Additionally, we would like to
acknowledge additional funding from the National Institute of General
Medical Sciences (A.M.S. and C.J.), National Institute of Allergy and
Infectious Diseases (N.N. 1K22AI113078-01), and the Department of
Biological Sciences at Purdue University (N.N.).
NR 44
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PU JOURNAL OF VISUALIZED EXPERIMENTS
PI CAMBRIDGE
PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA
SN 1940-087X
J9 JOVE-J VIS EXP
JI J. Vis. Exp.
PD JUL
PY 2016
IS 113
AR e53245
DI 10.3791/53245
PG 14
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DZ1TG
UT WOS:000385622600009
ER
PT J
AU Rosenheck, R
Leslie, D
Sint, K
Lin, HQ
Robinson, DG
Schooler, NR
Mueser, KT
Penn, DL
Addington, J
Brunette, MF
Correll, CU
Estroff, SE
Marcy, P
Robinson, J
Severe, J
Rupp, A
Schoenbaum, M
Kane, JM
AF Rosenheck, Robert
Leslie, Douglas
Sint, Kyaw
Lin, Haiqun
Robinson, Delbert G.
Schooler, Nina R.
Mueser, Kim T.
Penn, David L.
Addington, Jean
Brunette, Mary F.
Correll, Christoph U.
Estroff, Sue E.
Marcy, Patricia
Robinson, James
Severe, Joanne
Rupp, Agnes
Schoenbaum, Michael
Kane, John M.
TI Cost-Effectiveness of Comprehensive, Integrated Care for First Episode
Psychosis in the NIMH RAISE Early Treatment Program
SO SCHIZOPHRENIA BULLETIN
LA English
DT Article
DE schizophrenia; cost-effectiveness; quality adjusted life years
ID RANDOMIZED CONTROLLED-TRIAL; EARLY INTERVENTION SERVICE; NEGATIVE
SYNDROME SCALE; ADJUSTED LIFE-YEAR; HEALTH-CARE; CATCHMENT-AREA;
HOSPITAL USE; SCHIZOPHRENIA; DISORDERS; QUALITY
AB This study compares the cost-effectiveness of Navigate (NAV), a comprehensive, multidisciplinary, team-based treatment approach for first episode psychosis (FEP) and usual Community Care (CC) in a cluster randomization trial. Patients at 34 community treatment clinics were randomly assigned to either NAV (N = 223) or CC (N = 181) for 2 years. Effectiveness was measured as a one standard deviation change on the Quality of Life Scale (QLS-SD). Incremental cost effectiveness ratios were evaluated with bootstrap distributions. The Net Health Benefits Approach was used to evaluate the probability that the value of NAV benefits exceeded its costs relative to CC from the perspective of the health care system. The NAV group improved significantly more on the QLS and had higher outpatient mental health and antipsychotic medication costs. The incremental cost-effectiveness ratio was $ 12 081/QLS-SD, with a.94 probability that NAV was more cost-effective than CC at $ 40 000/QLS-SD. When converted to monetized Quality Adjusted Life Years, NAV benefits exceeded costs, especially at future generic drug prices.
C1 [Rosenheck, Robert; Sint, Kyaw; Lin, Haiqun] Yale Med Sch, Dept Psychiat & Publ Hlth, New Haven, CT USA.
[Leslie, Douglas] Penn State Coll Med, Dept Publ Hlth Sci, Hershey, PA USA.
[Robinson, Delbert G.; Schooler, Nina R.; Correll, Christoph U.; Marcy, Patricia; Kane, John M.] North Shore Long Isl Jewish, Zucker Hillside Hosp, Psychiat Res, Glen Oaks, NY USA.
[Robinson, Delbert G.; Correll, Christoph U.; Kane, John M.] Feinstein Inst Med Res, Manhasset, NY USA.
[Robinson, Delbert G.; Correll, Christoph U.; Kane, John M.] Hofstra North Shore LIJ Sch Med, Dept Psychiat, Hempstead, NY USA.
[Robinson, Delbert G.; Correll, Christoph U.; Kane, John M.] Hofstra North Shore LIJ Sch Med, Dept Mol Med, Hempstead, NY USA.
[Schooler, Nina R.; Correll, Christoph U.; Kane, John M.] Albert Einstein Coll Med, Dept Psychiat & Behav Sci, Bronx, NY 10467 USA.
[Schooler, Nina R.] Suny Downstate Med Ctr, Dept Psychiat, Brooklyn, NY 11203 USA.
[Mueser, Kim T.] Boston Univ, Dept Occupat Therapy, Ctr Psychiat Rehabil, Boston, MA 02215 USA.
[Mueser, Kim T.] Boston Univ, Dept Psychiat, Ctr Psychiat Rehabil, Boston, MA 02215 USA.
[Mueser, Kim T.] Boston Univ, Dept Psychol, Ctr Psychiat Rehabil, 64 Cummington St, Boston, MA 02215 USA.
[Penn, David L.] Univ North Carolina Chapel Hill, Dept Psychol, Chapel Hill, NC USA.
[Penn, David L.] Australian Catholic Univ, Sch Psychol, Melbourne, Vic, Australia.
[Addington, Jean] Univ Calgary, Hotchkiss Brain Inst, Dept Psychiat, Calgary, AB, Canada.
[Brunette, Mary F.] Geisel Sch Med Dartmouth, Lebanon, NH USA.
[Brunette, Mary F.] DHHS, Bur Behav Hlth, Concord, NH USA.
[Estroff, Sue E.] Univ N Carolina, Dept Social Med, Chapel Hill, NC USA.
[Robinson, James] Nathan S Kline Inst Psychiat Res, Orangeburg, NY USA.
[Severe, Joanne; Rupp, Agnes; Schoenbaum, Michael] NIMH, Rockville, MD 20857 USA.
RP Rosenheck, R (reprint author), VA Connecticut Hlth Care Syst, VA New England Mental Illness Res Educ & Clin Ctr, 950 Campbell Ave, West Haven, CT 06516 USA.
EM Robert.Rosenheck@Yale.Edu
FU American Recovery and Reinvestment Act; National Institute of Mental
Health [HHSN271200900019C]
FX This work was supported with funds from the American Recovery and
Reinvestment Act and from the National Institute of Mental Health, under
Contract No. HHSN271200900019C. The contents of this article are solely
the responsibility of the authors and do not necessarily represent the
views of National Institute of Mental Health or the US Department of
Health and Human Services.
NR 44
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U1 1
U2 1
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0586-7614
EI 1745-1701
J9 SCHIZOPHRENIA BULL
JI Schizophr. Bull.
PD JUL
PY 2016
VL 42
IS 4
BP 896
EP 906
DI 10.1093/schbul/sbv224
PG 11
WC Psychiatry
SC Psychiatry
GA DY7AF
UT WOS:000385280800011
PM 26834024
ER
PT J
AU Xu, Y
Yue, WH
Shugart, YY
Li, S
Cai, L
Li, Q
Cheng, ZH
Wang, GQ
Zhou, ZH
Jin, CH
Yuan, JM
Tian, L
Wang, J
Zhang, K
Zhang, KR
Liu, S
Song, YQ
Zhang, FQ
AF Xu, Yong
Yue, Weihua
Shugart, Yin Yao
Li, Sheng
Cai, Lei
Li, Qiang
Cheng, Zaohuo
Wang, Guoqiang
Zhou, Zhenhe
Jin, Chunhui
Yuan, Jianmin
Tian, Lin
Wang, Jun
Zhang, Kai
Zhang, Kerang
Liu, Sha
Song, Yuqing
Zhang, Fuquan
TI Exploring Transcription Factors-microRNAs Co-regulation Networks in
Schizophrenia
SO SCHIZOPHRENIA BULLETIN
LA English
DT Article
DE schizophrenia; transcriptional factor; microRNA; miR-124-3p; EGRl
ID GENE COEXPRESSION NETWORKS; R PACKAGE; COMMON VARIANTS; BRAIN DISORDERS;
CONFERRING RISK; HUMAN-DISEASE; EXPRESSION; MICROARRAY; DATABASE; GENOME
AB Background: Transcriptional factors (TFs) and microRNAs (miRNAs) have been recognized as 2 classes of principal gene regulators that may be responsible for genome coexpression changes observed in schizophrenia (SZ). Methods: This study aims to (1) identify differentially coexpressed genes (DCGs) in 3 mRNA expression microarray datasets; (2) explore potential interactions among the DCGs, and differentially expressed miRNAs identified in our dataset composed of early-onset SZ patients and healthy controls; (3) validate expression levels of some key transcripts; and (4) explore the druggability of DCGs using the curated database. Results: We detected a differential coexpression network associated with SZ and found that 9 out of the 12 regulators were replicated in either of the 2 other datasets. Leveraging the differentially expressed miRNAs identified in our previous dataset, we constructed a miRNA-TF-gene network relevant to SZ, including an EGR1-miR-1243p-SKIL feed-forward loop. Our real-time quantitative PCR analysis indicated the overexpression of miR-124-3p, the under expression of SKIL and EGR1 in the blood of SZ patients compared with controls, and the direction of change of miR-124-3p and SKIL mRNA levels in SZ cases were reversed after a 12-week treatment cycle. Our druggability analysis revealed that many of these genes have the potential to be drug targets. Conclusions: Together, our results suggest that coexpression network abnormalities driven by combinatorial and interactive action from TFs and miRNAs may contribute to the development of SZ and be relevant to the clinical treatment of the disease.
C1 [Xu, Yong; Zhang, Kerang; Liu, Sha] Shanxi Med Univ, Hosp 1, Clin Med Coll 1, Dept Psychiat, Taiyuan, Peoples R China.
[Yue, Weihua; Song, Yuqing] Peking Univ, Hosp 6, Inst Mental Hlth, Dept Psychiat, Beijing, Peoples R China.
[Yue, Weihua] Peking Univ, Key Lab Mental Hlth, Minist Hlth, Beijing, Peoples R China.
[Yue, Weihua] Peking Univ, Natl Clin Res Ctr Mental Disorders, Beijing, Peoples R China.
[Shugart, Yin Yao] NIMH, Unit Stat Genom, Intramural Res Program, NIH, Bethesda, MD 20892 USA.
[Li, Sheng; Cai, Lei] Shanghai Jiao Tong Univ, Minist Educ, BioX Inst, Key Lab Genet Dev & Neuropsychiat Disorders, Shanghai, Peoples R China.
[Li, Qiang] Fudan Univ, Childrens Hosp, Shanghai Key Lab Birth Defect, Shanghai, Peoples R China.
[Cheng, Zaohuo; Wang, Guoqiang; Zhou, Zhenhe; Jin, Chunhui; Yuan, Jianmin; Tian, Lin; Wang, Jun; Zhang, Kai; Zhang, Fuquan] Nanjing Med Univ, Dept Psychiat, Wuxi Mental Hlth Ctr, 156 Qianrong Rd, Wuxi, Peoples R China.
RP Zhang, FQ (reprint author), Nanjing Med Univ, Dept Psychiat, Wuxi Mental Hlth Ctr, 156 Qianrong Rd, Wuxi, Peoples R China.
EM zhangfq@njmu.edu.cn
FU National Natural Science Foundation of China [81271482, 81471364,
81571319, 81222017]; Program for New Century Excellent Talents in
University [NCET-12-1036]; Construction Plan for Shanxi Science &
Technology Infrastructure Platforms [2015091002-0102]; Intramural
Research Program of National Institute of Mental Health, National
Institutes of Health [MH002929-05]
FX This work was supported by National Natural Science Foundation of China
(81271482, 81471364, 81571319, 81222017), Program for New Century
Excellent Talents in University (NCET-12-1036), and Construction Plan
for Shanxi Science & Technology Infrastructure Platforms
(2015091002-0102). Shugart was supported by the Intramural Research
Program of National Institute of Mental Health, National Institutes of
Health (MH002929-05).
NR 58
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U1 2
U2 2
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0586-7614
EI 1745-1701
J9 SCHIZOPHRENIA BULL
JI Schizophr. Bull.
PD JUL
PY 2016
VL 42
IS 4
BP 1037
EP 1045
DI 10.1093/schbul/sbv170
PG 9
WC Psychiatry
SC Psychiatry
GA DY7AF
UT WOS:000385280800026
PM 26609121
ER
PT J
AU Bhadriraju, K
Halter, M
Amelot, J
Bajcsy, P
Chalfoun, J
Vandecreme, A
Mallon, BS
Park, KY
Sista, S
Elliott, JT
Plant, AL
AF Bhadriraju, Kiran
Halter, Michael
Amelot, Julien
Bajcsy, Peter
Chalfoun, Joe
Vandecreme, Antoine
Mallon, Barbara S.
Park, Kye-yoon
Sista, Subhash
Elliott, John T.
Plant, Anne L.
TI Large-scale time-lapse microscopy of Oct4 expression in human embryonic
stem cell colonies
SO STEM CELL RESEARCH
LA English
DT Article
DE Fluorescence microscopy; Stem cells; Live cell imaging; Cell therapy;
Pluripotency
ID SELF-RENEWAL; TRANSCRIPTIONAL REGULATION; FLUORESCENCE MICROSCOPE; ES
CELLS; PLURIPOTENCY; NANOG; DIFFERENTIATION; SOX2; ENHANCER; NETWORK
AB Identification and quantification of the characteristics of stemcell preparations is critical for understanding stem cell biology and for the development and manufacturing of stem cell based therapies. We have developed image analysis and visualization software that allows effective use of time-lapse microscopy to provide spatial and dynamic information from large numbers of human embryonic stem cell colonies. To achieve statistically relevant sampling, we examined >680 colonies from 3 different preparations of cells over 5 days each, generating a total experimental dataset of 0.9 terabyte (TB). The 0.5 Giga-pixel images at each time point were represented by multi-resolution pyramids and visualized using the Deep Zoom Javascript library extended to support viewing Giga-pixel images over time and extracting data on individual colonies. We present a methodology that enables quantification of variations in nominally-identical preparations and between colonies, correlation of colony characteristics with Oct4 expression, and identification of rare events. (C) Published by Elsevier B.V.
C1 [Bhadriraju, Kiran] Univ Maryland, Dept Bioengn, College Pk, MD 20742 USA.
[Halter, Michael; Sista, Subhash; Elliott, John T.; Plant, Anne L.] NIST, Biosyst & Biomat Div, Mat Measurement Lab, Gaithersburg, MD 20899 USA.
[Amelot, Julien; Bajcsy, Peter; Chalfoun, Joe; Vandecreme, Antoine] NIST, Software Syst Div, Informat Technol Lab, Gaithersburg, MD 20899 USA.
[Mallon, Barbara S.; Park, Kye-yoon] NINDS, NIH Stem Cell Unit, Div Intramural Res, NIH,US Dept Hlth & Human Serv, Bethesda, MD 20892 USA.
RP Halter, M (reprint author), NIST, Biosyst & Biomat Div, Mat Measurement Lab, Gaithersburg, MD 20899 USA.
FU ISAC Scholars Program
FX We would like to thank Michael Majurski, Jing Gao, and Mylene Simon for
data processing and web application development. We would also like to
thank Steven Lund and James Filliben for help with statistical analysis.
MH acknowledges support from the ISAC Scholars Program.
NR 36
TC 1
Z9 1
U1 6
U2 6
PU ELSEVIER SCIENCE BV
PI AMSTERDAM
PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
SN 1873-5061
EI 1876-7753
J9 STEM CELL RES
JI Stem Cell Res.
PD JUL
PY 2016
VL 17
IS 1
BP 122
EP 129
DI 10.1016/j.scr.2016.05.012
PG 8
WC Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Cell
Biology
SC Cell Biology; Biotechnology & Applied Microbiology
GA DY6ZF
UT WOS:000385278000024
PM 27286574
ER
PT J
AU Sirenko, SG
Maltsev, VA
Yaniv, Y
Bychkov, R
Yaeger, D
Vinogradova, T
Spurgeon, HA
Lakatta, EG
AF Sirenko, Syevda G.
Maltsev, Victor A.
Yaniv, Yael
Bychkov, Rostislav
Yaeger, Daniel
Vinogradova, Tatiana
Spurgeon, Harold A.
Lakatta, Edward G.
TI Electrochemical Na+ and Ca2+ gradients drive coupled-clock regulation of
automaticity of isolated rabbit sinoatrial nodal pacemaker cells
SO AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
LA English
DT Article
DE cardiac automaticity; sodium; calcium; electrochemical driving forces
ID CARDIAC PURKINJE-FIBERS; RYANODINE RECEPTORS; DIGITALIS TOXICITY;
INTRACELLULAR PH; REDOX MODIFICATION; SODIUM ACTIVITY; ATPASE ACTIVITY;
SINUS NODE; HEART; OUABAIN
AB Coupling of an intracellular Ca2+ clock to surface membrane ion channels, i.e., a "membrane clock," via coupling of electrochemical Na+ and Ca2+ gradients (E-Na and E-Ca, respectively) has been theorized to regulate sinoatrial nodal cell (SANC) normal automaticity. To test this hypothesis, we measured responses of [Na+](i), [Ca2+](i), membrane potential, action potential cycle length (APCL), and rhythm in rabbit SANCs to Na+ /K+ pump inhibition by the digitalis glycoside, digoxigenin (DG, 10-20 mu mol/l). Initial small but significant increases in [Na+](i) and [Ca2+](i) and reductions in E-Na and E-Ca in response to DG led to a small reduction in maximum diastolic potential (MDP), significantly enhanced local diastolic Ca2+ releases (LCRs), and reduced the average APCL. As [Na+](i) and [Ca2+](i) continued to increase at longer times following DG exposure, further significant reductions in MDP, E-Na, and E-Ca occurred; LCRs became significantly reduced, and APCL became progressively and significantly prolonged. This was accompanied by increased APCL variability. We also employed a coupled-clock numerical model to simulate changes in E-Na and E-Ca simultaneously with ion currents not measured experimentally. Numerical modeling predicted that, as the E-Na and E-Ca monotonically reduced over time in response to DG, ion currents (I-CaL, I-CaT, I-f, I-Kr, and I-bNa) monotonically decreased. In parallel with the biphasic APCL, diastolic I-NCX manifested biphasic changes; initial I-NCX increase attributable to enhanced LCR ensemble Ca2+ signal was followed by I-NCX reduction as E-NCX (E-NCX = 3E(Na) - 2E(Ca)) decreased. Thus SANC automaticity is tightly regulated by E-Na, E-Ca, and E-NCX via a complex interplay of numerous key clock components that regulate SANC clock coupling.
C1 [Sirenko, Syevda G.; Maltsev, Victor A.; Yaniv, Yael; Bychkov, Rostislav; Yaeger, Daniel; Vinogradova, Tatiana; Spurgeon, Harold A.; Lakatta, Edward G.] NIA, Cardiovasc Sci Lab, NIH, Intramural Res Program, Baltimore, MD 21224 USA.
[Yaniv, Yael] Technion IIT, Biomed Engn Fac, Haifa, Israel.
[Bychkov, Rostislav] Univ Cent Caribe, Bayamon, PR USA.
RP Lakatta, EG (reprint author), NIA, Cardiovasc Sci Lab, NIH, Biomed Res Ctr, 51 Bayview Blvd, Baltimore, MD 21224 USA.
EM lakattae@grc.nia.nih.gov
FU Intramural Research Program of the NIH, National Institute on Aging
FX This work was supported by the Intramural Research Program of the NIH,
National Institute on Aging.
NR 57
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U2 0
PU AMER PHYSIOLOGICAL SOC
PI BETHESDA
PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA
SN 0363-6135
EI 1522-1539
J9 AM J PHYSIOL-HEART C
JI Am. J. Physiol.-Heart Circul. Physiol.
PD JUL
PY 2016
VL 311
IS 1
BP H251
EP H267
DI 10.1152/ajpheart.00667.2015
PG 17
WC Cardiac & Cardiovascular Systems; Physiology; Peripheral Vascular
Disease
SC Cardiovascular System & Cardiology; Physiology
GA DY3CZ
UT WOS:000384969700026
PM 27208164
ER
PT J
AU Karschner, EL
Swortwood, MJ
Hirvonen, J
Goodwin, RS
Bosker, WM
Ramaekers, JG
Huestis, MA
AF Karschner, Erin L.
Swortwood, Madeleine J.
Hirvonen, Jussi
Goodwin, Robert S.
Bosker, Wendy M.
Ramaekers, Johannes G.
Huestis, Marilyn A.
TI Extended plasma cannabinoid excretion in chronic frequent cannabis
smokers during sustained abstinence and correlation with psychomotor
performance
SO DRUG TESTING AND ANALYSIS
LA English
DT Article
DE cannabinoid; Delta(9)-tetrahydrocannabinol; cannabis; chronic;
abstinence
ID MASS-SPECTROMETRY; GLUCURONIDATED CANNABINOIDS; SIMULTANEOUS
QUANTIFICATION; GAS-CHROMATOGRAPHY; MARIJUANA USERS; HUMAN-URINE; ORAL
FLUID; BLOOD; DELTA-9-TETRAHYDROCANNABINOL; THC
AB Cannabis smoking increases motor vehicle accident risk. Empirically defined cannabinoid detection windows are important to drugged driving legislation. Our aims were to establish plasma cannabinoid detection windows in frequent cannabis smokers and to determine if residual cannabinoid concentrations were correlated with psychomotor performance. Twenty-eight male chronic frequent cannabis smokers resided on a secure research unit for up to 33 days with daily blood collection. Plasma specimens were analyzed for Delta(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), and 11-nor-9-carboxy-THC (THCCOOH) by gas chromatography-mass spectrometry. Critical tracking and divided attention tasks were administered at baseline (after overnight stay to ensure lack of acute intoxication) and after 1, 2, and 3 weeks of cannabis abstinence. Twenty-seven of the twenty-eight participants were THC-positive at admission (median 4.2 mu g/L). THC concentrations significantly decreased 24 h after admission, but were still >= 2 mu g/L in 16 of the 28 participants 48 h after admission. THC was detected in 3 of 5 specimens on day 30. The last positive 11-OH-THC specimen was 15 days after admission. THCCOOH was measureable in 4 of 5 participants after 30 days of abstinence. Years of prior cannabis use significantly correlated with THC concentrations on admission, and days 7 and 14. Tracking error, evaluated by the Divided Attention Task, was the only evaluated psychomotor assessment significantly correlated with cannabinoid concentrations at baseline and day 8 (11-OH-THC only). Median THC was 0.3 mu g/L in 5 chronic frequent cannabis smokers' plasma samples after 30 days of sustained abstinence. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.
C1 [Karschner, Erin L.; Swortwood, Madeleine J.; Goodwin, Robert S.; Bosker, Wendy M.; Huestis, Marilyn A.] NIDA, Chem & Drug Metab, Intramural Res Program, NIH, 251 Bayview Blvd,Suite 05A721, Baltimore, MD 21224 USA.
[Karschner, Erin L.] Armed Forces Med Examiner Syst, Div Forens Toxicol, 115 Purple Heart Dr, Dover Afb, DE 19902 USA.
[Hirvonen, Jussi] NIMH, Mol Imaging Branch, IRP, NIH, 6001 Execut Blvd, Bethesda, MD 20892 USA.
[Hirvonen, Jussi] Univ Turku, Dept Diagnost Radiol, Turun 20014, Finland.
[Goodwin, Robert S.] 7 Church Lane,Suite 15A, Pikesville, MD 21208 USA.
[Bosker, Wendy M.] Forschungszentrum Julich, Inst Neurosci & Med 4, D-52425 Julich, Germany.
[Bosker, Wendy M.; Ramaekers, Johannes G.] Maastricht Univ, Fac Psychol & Neurosci, Dept Neuropsychol & Psychopharmacol, NL-6211 LK Maastricht, Netherlands.
RP Huestis, MA (reprint author), NIDA, Chem & Drug Metab, Intramural Res Program, NIH,Biomed Res Ctr, 251 Bayview Blvd,Room 05A721, Baltimore, MD 21224 USA.
EM mhuestis@intra.nida.nih.gov
FU Intramural Research Programs of the National Institute on Drug Abuse;
National Institutes of Mental Health, National Institutes of Health
FX This research was funded by the Intramural Research Programs of the
National Institute on Drug Abuse and National Institutes of Mental
Health, National Institutes of Health.
NR 56
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U1 5
U2 5
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1942-7603
EI 1942-7611
J9 DRUG TEST ANAL
JI Drug Test. Anal.
PD JUL
PY 2016
VL 8
IS 7
BP 682
EP 689
DI 10.1002/dta.1825
PG 8
WC Biochemical Research Methods; Chemistry, Analytical; Pharmacology &
Pharmacy
SC Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy
GA DY0RI
UT WOS:000384803500009
PM 26097154
ER
PT J
AU Hartman, RL
Brown, TL
Milavetz, G
Spurgin, A
Gorelick, DA
Gaffney, G
Huestis, MA
AF Hartman, Rebecca L.
Brown, Timothy L.
Milavetz, Gary
Spurgin, Andrew
Gorelick, David A.
Gaffney, Gary
Huestis, Marilyn A.
TI Controlled vaporized cannabis, with and without alcohol: subjective
effects and oral fluid-blood cannabinoid relationships
SO DRUG TESTING AND ANALYSIS
LA English
DT Article
DE cannabis; alcohol; subjective; blood; oral fluid
ID WHOLE-BLOOD; SMOKED CANNABIS; DELTA(9)-TETRAHYDROCANNABINOL THC;
PHARMACOKINETIC PROPERTIES; PSYCHOMOTOR PERFORMANCE; MASS-SPECTROMETRY;
MARIJUANA SMOKING; HUMAN VOLUNTEERS; MOTOR CONTROL; PLASMA
AB Vaporized cannabis and concurrent cannabis and alcohol intake are commonplace. We evaluated the subjective effects of cannabis, with and without alcohol, relative to blood and oral fluid (OF, advantageous for cannabis exposure screening) cannabinoid concentrations and OF/blood and OF/plasma vaporized-cannabinoid relationships. Healthy adult occasional-to-moderate cannabis smokers received a vaporized placebo or active cannabis (2.9% and 6.7% Delta(9)-tetrahydrocannabinol, THC) with or without oral low-dose alcohol (similar to 0.065g/210L peak breath alcohol concentration [BrAC]) in a within-subjects design. Blood and OF were collected up to 8.3 h post-dose and subjective effects measured at matched time points with visual-analogue scales and 5-point Likert scales. Linear mixed models evaluated subjective effects by THC concentration, BrAC, and interactions. Effects by time point were evaluated by dose-wise analysis of variance (ANOVA). OF versus blood or plasma cannabinoid ratios and correlations were evaluated in paired-positive specimens. Nineteen participants (13 men) completed the study. Blood THC concentration or BrAC significantly associated with subjective effects including 'high', while OF contamination prevented significant OF concentration associations <1.4 h post-dose. Subjective effects persisted through 3.3-4.3 h, with alcohol potentiating the duration of the cannabis effects. Effect-versus-THC concentration and effect-versus-alcohol concentration hystereses were counterclockwise and clockwise, respectively. OF/blood and OF/plasma THC significantly correlated (all Spearman r >= 0.71), but variability was high. Vaporized cannabis subjective effects were similar to those previously reported after smoking, with duration extended by concurrent alcohol. Cannabis intake was identified by OF testing, but OF concentration variability limited interpretation. Blood THC concentrations were more consistent across subjects and more accurate at predicting cannabis' subjective effects. Copyright (C) 2015 John Wiley & Sons, Ltd.
C1 [Hartman, Rebecca L.; Huestis, Marilyn A.] NIDA, Chem & Drug Metab, Intramural Res Program, NIH, Baltimore, MD USA.
[Hartman, Rebecca L.] Univ Maryland, Toxicol Program, Baltimore, MD 21201 USA.
[Brown, Timothy L.] Univ Iowa, Natl Adv Driving Simulator, Iowa City, IA USA.
[Milavetz, Gary; Spurgin, Andrew] Univ Iowa, Coll Pharm, Iowa City, IA 52242 USA.
[Gorelick, David A.] Univ Maryland, Sch Med, Dept Psychiat, Baltimore, MD 21201 USA.
[Gaffney, Gary] Univ Iowa, Carver Coll Med, Iowa City, IA USA.
RP Huestis, MA (reprint author), Biomed Res Ctr, Suite 200,Room 05A-721,251 Bayview Blvd, Baltimore, MD 21224 USA.
EM mhuestis@intra.nida.nih.gov
FU Intramural Research Program, National Institute on Drug Abuse, NIH;
United States Office of National Drug Control Policy; National Highway
Traffic Safety Administration
FX We thank the nurses and staff of the University of Iowa Clinical
Research Unit and National Advanced Driving Simulator, especially Cheryl
Roe, Jennifer Henderson, Rose Schmitt, and Kayla Smith, for excellent
contributions to successful study completion. We also thank Drs Dereece
Smither and Richard Compton, National Highway Traffic Safety
Administration, for invaluable input. We acknowledge University of
Maryland, Baltimore Toxicology Program, and Graduate Partnership
Program, National Institutes of Health (NIH). Quantisal TM and Volcano
(R) devices were provided by the manufacturers to NIH through Materials
Transfer Agreements, but manufacturers played no role in study design,
data analysis, or manuscript writing. Research was funded by the
Intramural Research Program, National Institute on Drug Abuse, NIH, the
United States Office of National Drug Control Policy, and the National
Highway Traffic Safety Administration.
NR 53
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U1 5
U2 5
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1942-7603
EI 1942-7611
J9 DRUG TEST ANAL
JI Drug Test. Anal.
PD JUL
PY 2016
VL 8
IS 7
BP 690
EP 701
DI 10.1002/dta.1839
PG 12
WC Biochemical Research Methods; Chemistry, Analytical; Pharmacology &
Pharmacy
SC Biochemistry & Molecular Biology; Chemistry; Pharmacology & Pharmacy
GA DY0RI
UT WOS:000384803500010
PM 26257143
ER
PT J
AU Arai, AE
AF Arai, Andrew E.
TI Area at risk in acute myocardial infarction: oedema imaging and
species-specific findings
SO EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
LA English
DT Editorial Material
ID ISCHEMIC AREA; BIMODAL PATTERN; AT-RISK; SALVAGE; ISCHEMIA/REPERFUSION;
GADOLINIUM; MRI
C1 [Arai, Andrew E.] NHLBI, Dept Hlth & Human Serv, NIH, Bldg 10,Room B1D416,MSC 1061,10 Ctr Dr, Bethesda, MD 20892 USA.
RP Arai, AE (reprint author), NHLBI, Dept Hlth & Human Serv, NIH, Bldg 10,Room B1D416,MSC 1061,10 Ctr Dr, Bethesda, MD 20892 USA.
EM araia@nih.gov
NR 16
TC 0
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U1 0
U2 0
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 2047-2404
EI 2047-2412
J9 EUR HEART J-CARD IMG
JI Eur. Heart J.-Cardiovasc. Imaging
PD JUL 1
PY 2016
VL 17
IS 7
BP 754
EP 755
DI 10.1093/ehjci/jew074
PG 2
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA DX9OP
UT WOS:000384725300009
PM 27145801
ER
PT J
AU Kuhl, JT
George, RT
Mehra, VC
Linde, JJ
Chen, M
Arai, AE
Di Carli, M
Kitagawa, K
Dewey, M
Lima, JAC
Kofoed, KF
AF Kuhl, Jorgen Tobias
George, Richard T.
Mehra, Vishal C.
Linde, Jesper J.
Chen, Marcus
Arai, Andrew E.
Di Carli, Marcelo
Kitagawa, Kakuya
Dewey, Marc
Lima, Joao A. C.
Kofoed, Klaus Fuglsang
TI Endocardial-epicardial distribution of myocardial perfusion reserve
assessed by multidetector computed tomography in symptomatic patients
without significant coronary artery disease: insights from the CORE320
multicentre study
SO EUROPEAN HEART JOURNAL-CARDIOVASCULAR IMAGING
LA English
DT Article
DE myocardial perfusion; multi-detector computed tomography; coronary
artery disease
ID POSITRON-EMISSION-TOMOGRAPHY; BLOOD-FLOW; INSULIN-RESISTANCE; CT;
ANGIOGRAPHY; HETEROGENEITY; DYSFUNCTION; ISCHEMIA; HUMANS; RISK
AB Aim Previous animal studies have demonstrated differences in perfusion and perfusion reserve between the subendocardium and subepicardium. 320-row computed tomography (CT) with sub-millimetre spatial resolution allows for the assessment of transmural differences in myocardial perfusion reserve (MPR) in humans. We aimed to test the hypothesis that MPR in all myocardial layers is determined by age, gender, and cardiovascular risk profile in patients with ischaemic symptoms or equivalent but without obstructive coronary artery disease (CAD).
Methods and results A total of 149 patients enrolled in the CORE320 study with symptoms or signs of myocardial ischaemia and absence of significant CAD by invasive coronary angiography were scanned with static rest and stress CT perfusion. Myocardial attenuation densities were assessed at rest and during adenosine stress, segmented into 3 myocardial layers and 13 segments. MPR was higher in the subepicardium compared with the subendocardium (124% interquartile range [45, 235] vs. 68% [22,102], P < 0.001). Moreover, MPR in the septum was lower than in the inferolateral and anterolateral segments of the myocardium (55% [19, 104] vs. 89% [37, 168] and 124% [54, 270], P < 0.001). By multivariate analysis, high body mass index was significantly associated with reduced MPR in all myocardial layers when adjusted for cardiovascular risk factors (P = 0.02).
Conclusion In symptomatic patients without significant coronary artery stenosis, distinct differences in endocardial-epicardial distribution of perfusion reserve may be demonstrated with static CT perfusion. Low MPR in all myocardial layers was observed specifically in obese patients.
C1 [Kuhl, Jorgen Tobias; Linde, Jesper J.; Kofoed, Klaus Fuglsang] Univ Copenhagen, Dept Cardiol, Ctr Heart 2012, Rigshosp, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
[George, Richard T.; Mehra, Vishal C.; Lima, Joao A. C.] Johns Hopkins Univ, Dept Med, Baltimore, MD USA.
[Mehra, Vishal C.; Chen, Marcus; Arai, Andrew E.] NHLBI, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Di Carli, Marcelo] Brigham & Womens Hosp, 75 Francis St, Boston, MA 02115 USA.
[Kitagawa, Kakuya] Mie Univ Hosp, Tsu, Mie, Japan.
[Dewey, Marc] Charite, Berlin, Germany.
[Kofoed, Klaus Fuglsang] Univ Copenhagen, Dept Radiol, Rigshosp, Copenhagen, Denmark.
RP Kuhl, JT (reprint author), Univ Copenhagen, Dept Cardiol, Ctr Heart 2012, Rigshosp, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
EM tobiaskh@gmail.com
FU Toshiba Medical Systems Corporation
FX The study sponsor, Toshiba Medical Systems Corporation, was not involved
in any stage of the study design, data acquisition, data analysis, or
manuscript preparation.
NR 28
TC 0
Z9 0
U1 0
U2 0
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 2047-2404
EI 2047-2412
J9 EUR HEART J-CARD IMG
JI Eur. Heart J.-Cardiovasc. Imaging
PD JUL 1
PY 2016
VL 17
IS 7
BP 779
EP 787
DI 10.1093/ehjci/jev206
PG 9
WC Cardiac & Cardiovascular Systems
SC Cardiovascular System & Cardiology
GA DX9OP
UT WOS:000384725300015
PM 26341292
ER
PT J
AU Sandfort, V
Ahlman, MA
Jones, EC
Selwaness, M
Chen, MY
Folio, LR
Bluemke, DA
AF Sandfort, Veit
Ahlman, Mark A.
Jones, Elizabeth C.
Selwaness, Mariana
Chen, Marcus Y.
Folio, Les R.
Bluemke, David A.
TI High pitch third generation dual-source CT: Coronary and cardiac
visualization on routine chest CT
SO JOURNAL OF CARDIOVASCULAR COMPUTED TOMOGRAPHY
LA English
DT Article
DE CT; Cardiac imaging; Incidental findings
ID COMPUTED TOMOGRAPHIC ANGIOGRAPHY; PULMONARY NODULES; IMAGE QUALITY;
ARTERY-DISEASE; DIAGNOSIS
AB Background: Chest CT scans are frequently performed in radiology departments but have not previously contained detailed depiction of cardiac structures.
Objectives: To evaluate myocardial and coronary visualization on high-pitch non-gated CT of the chest using 3rd generation dual-source computed tomography (CT).
Methods: Cardiac anatomy of patients who had 3rd generation, non-gated high pitch contrast enhanced chest CT and who also had prior conventional (low pitch) chest CT as part of a chest abdomen pelvis exam was evaluated. Cardiac image features were scored by reviewers blinded to diagnosis and pitch. Paired analysis was performed.
Results: 3862 coronary segments and 2220 cardiac structures were evaluated by two readers in 222 CT scans. Most patients (97.2%) had chest CT for oncologic evaluation. The median pitch was 2.34 (IQR 2.05, 2.65) in high pitch and 0.8 (IQR 0.8, 0.8) in low pitch scans (p < 0.001). High pitch CT showed higher image visualization scores for all cardiovascular structures compared with conventional pitch scans (p < 0.0001). Coronary arteries were visualized in 9 coronary segments per exam in high pitch scans versus 2 segments for conventional pitch (p < 0.0001). Radiation exposure was lower in the high pitch group compared with the conventional pitch group (median CTDIvol 10.83 vs. 12.36 mGy and DLP 790 vs. 827 mGycm respectively, p < 0.01 for both) with comparable image noise (p = 0.43).
Conclusion: Myocardial structure and coronary arteries are frequently visualized on non-gated 3rd generation chest CT. These results raise the question of whether the heart and coronary arteries should be routinely interpreted on routine chest CT that is otherwise obtained for non-cardiac indications. Published by Elsevier Inc. on behalf of Society of Cardiovascular Computed Tomography.
C1 [Sandfort, Veit; Ahlman, Mark A.; Jones, Elizabeth C.; Selwaness, Mariana; Chen, Marcus Y.; Folio, Les R.; Bluemke, David A.] NIH, Radiol & Imaging Sci, Ctr Clin, 9000 Rockville Pike,10 Ctr Dr,Bld 10,Room 1C356, Bethesda, MD 20892 USA.
RP Bluemke, DA (reprint author), NIH, Radiol & Imaging Sci, Ctr Clin, 9000 Rockville Pike,10 Ctr Dr,Bld 10,Room 1C356, Bethesda, MD 20892 USA.
EM bluemked@cc.nih.gov
OI Bluemke, David/0000-0002-8323-8086
FU NIH intramural program [CL090019, EB000072]
FX Funding was provided by the NIH (grant numbers: CL090019, EB000072)
intramural program.
NR 25
TC 1
Z9 1
U1 0
U2 0
PU ELSEVIER SCIENCE INC
PI NEW YORK
PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
SN 1934-5925
J9 J CARDIOVASC COMPUT
JI J. Cardiovasc. Comput. Tomogr.
PD JUL-AUG
PY 2016
VL 10
IS 4
BP 282
EP 288
DI 10.1016/j.jcct.2016.03.007
PG 7
WC Cardiac & Cardiovascular Systems; Radiology, Nuclear Medicine & Medical
Imaging
SC Cardiovascular System & Cardiology; Radiology, Nuclear Medicine &
Medical Imaging
GA DY3SN
UT WOS:000385014800003
PM 27133589
ER
PT J
AU Gudino, N
Duan, Q
de Zwart, JA
Murphy-Boesch, J
Dodd, SJ
Merkle, H
van Gelderen, P
Duyn, JH
AF Gudino, Natalia
Duan, Qi
de Zwart, Jacco A.
Murphy-Boesch, Joe
Dodd, Stephen J.
Merkle, Hellmut
van Gelderen, Peter
Duyn, Jeff H.
TI Optically Controlled Switch-Mode Current-Source Amplifiers for On-Coil
Implementation in High-Field Parallel Transmission
SO MAGNETIC RESONANCE IN MEDICINE
LA English
DT Article
DE parallel transmission; high-field MRI; RF amplifiers
ID CURRENT AMPLITUDE; RF POWER; INHOMOGENEITY; FEEDBACK; ARRAYS; HEAD; MRI
AB Purpose: We tested the feasibility of implementing parallel transmission (pTX) for high-field MRI using a radiofrequency (RF) amplifier design to be located on or in the immediate vicinity of an RF transmit coil.
Method: We designed a current-source switch-mode amplifier based on miniaturized, nonmagnetic electronics. Optical RF carrier and envelope signals to control the amplifier were derived, through a custom-built interface, from the RF source accessible in the scanner control. Amplifier performance was tested by benchtop measurements as well as with imaging at 7T (300 MHz) and 11.7 T (500 MHz). The ability to perform pTX was evaluated by measuring interchannel coupling and phase adjustment in a two-channel setup.
Results: The amplifier delivered in excess of 44 W RF power and caused minimal interference with MRI. The interface derived accurate optical control signals with carrier frequencies ranging from 64 to 750 MHz. Decoupling better than 14 dB was obtained between two coil loops separated by only 1 cm. Application to MRI was demonstrated by acquiring artifact-free images at 7 T and 11.7 T.
Conclusion: We propose an optically controlled miniaturized RF amplifier for on-coil implementation at high fields that should facilitate implementation of high-density pTX arrays. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.
C1 [Gudino, Natalia; Duan, Qi; de Zwart, Jacco A.; Murphy-Boesch, Joe; Dodd, Stephen J.; Merkle, Hellmut; van Gelderen, Peter; Duyn, Jeff H.] NINDS, Lab Funct & Mol Imaging, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
RP Gudino, N (reprint author), NINDS, Adv MRI Sect, LFMI, 10 Ctr Dr,Bld 10,B1D728, Bethesda, MD 20892 USA.
EM natalia.gudino@nih.gov
RI Duan, Qi/J-7916-2016
OI Duan, Qi/0000-0002-2407-6611
FU National Institute of Neurological Disorders and Stroke
FX Grant sponsor: National Institute of Neurological Disorders and Stroke,
Intramural Research Program.
NR 24
TC 1
Z9 1
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0740-3194
EI 1522-2594
J9 MAGN RESON MED
JI Magn. Reson. Med.
PD JUL
PY 2016
VL 76
IS 1
BP 340
EP 349
DI 10.1002/mrm.25857
PG 10
WC Radiology, Nuclear Medicine & Medical Imaging
SC Radiology, Nuclear Medicine & Medical Imaging
GA DY3MK
UT WOS:000384996900034
PM 26256671
ER
PT J
AU Klinman, DM
Sato, T
Shimosato, T
AF Klinman, Dennis M.
Sato, Takashi
Shimosato, Takeshi
TI Use of nanoparticles to deliver immunomodulatory oligonucleotides
SO WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
LA English
DT Review
ID CELL LUNG-CANCER; RECEPTOR 9 AGONIST; BACTERIAL-DNA;
CPG-OLIGODEOXYNUCLEOTIDES; CARBONATE APATITE; DRUG-DELIVERY; TLR9
AGONIST; PLASMID DNA; COMPUTED-TOMOGRAPHY; GOLD NANOPARTICLES
AB Synthetic oligonucleotides (ODNs) containing unmethylated 'CpG motifs' stimulate the innate immune system to produce cytokines, chemokines, and polyreactive antibodies. CpG ODNs have shown promise as vaccine adjuvants and for the treatment of infectious diseases and cancer. The immunostimulatory activity of CpG ODNs is inhibited by DNA-containing 'suppressive' motifs. ODNs expressing suppressive motifs (Sup ODNs) reduce ongoing immune reactions and show promise in the treatment of autoimmune and inflammatory diseases. This work reviews recent progress in the use of nanoparticles as carriers of CpG and Sup ODNs to target their delivery to the GI tract and lungs. (C) 2015 Wiley Periodicals, Inc.
C1 [Klinman, Dennis M.] Frederick Natl Lab Canc Res, Canc & Inflammat Program, Frederick, MD 21702 USA.
[Sato, Takashi] Yokohama City Univ, Dept Internal Med & Clin Immunol, Grad Sch Med, Yokohama, Kanagawa, Japan.
[Shimosato, Takeshi] Shinshu Univ, Interdisciplinary Grad Sch Sci & Technol, Kamiina, Japan.
RP Klinman, DM (reprint author), Frederick Natl Lab Canc Res, Canc & Inflammat Program, Frederick, MD 21702 USA.
EM klinmand@mail.nih.gov
FU Intramural Research Program of the NIH, NCI
FX This work was supported by the Intramural Research Program of the NIH,
NCI. The content of this publication does not necessarily reflect the
views or policies of the Department of Health and Human Services, nor
does mention of trade names, commercial products, or organizations imply
endorsement by the United States government. The funders had no role in
study design, data collection and analysis, decision to publish, or
preparation of the article. The assertions herein are the private ones
of the authors and are not to be construed as official or as reflecting
the views of the National Cancer Institute at large.
NR 59
TC 1
Z9 1
U1 7
U2 7
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1939-5116
EI 1939-0041
J9 WIRES NANOMED NANOBI
JI Wiley Interdiscip. Rev.-Nanomed. Nanobiotechnol.
PD JUL-AUG
PY 2016
VL 8
IS 4
BP 631
EP 637
DI 10.1002/wnan.1382
PG 7
WC Nanoscience & Nanotechnology; Medicine, Research & Experimental
SC Science & Technology - Other Topics; Research & Experimental Medicine
GA DY3XN
UT WOS:000385031200008
PM 26663867
ER
PT J
AU Bayefsky, MJ
Berkman, BE
AF Bayefsky, Michelle J.
Berkman, Benjamin E.
TI The Ethics of Allocating Uterine Transplants
SO CAMBRIDGE QUARTERLY OF HEALTHCARE ETHICS
LA English
DT Article
DE uterine transplantation; infertility; allocation; scarce supply;
eligibility; motivation; age; child-rearing capacity
ID ADVANCED MATERNAL AGE; UTERUS TRANSPLANTATION; MONTREAL-CRITERIA;
INFERTILITY; FEASIBILITY; IMPACT; RISKS
AB In September 2014, a healthy male child was born in Sweden following a successful uterine transplantation (UTx). The event brought hope to many women without functional uteruses around the world. Having a child with a transplanted uterus is now possible, and as knowledge of the procedure proliferates and interest in UTx grows, it is important to begin thinking about how a scarce supply of uteruses will be allocated. This article represents a first discussion of the range of factors that must be considered in answering the allocation question. The primary issues addressed are (1) the motivation to seek treatment, (2) allocation by age, (3) child-rearing capacity, and (4) the amount of infertility treatment required. A set of eligibility and ranking criteria are presented. These criteria are not exhaustive but are intended to spark discussion about how uteruses can be allocated in a just manner.
C1 [Bayefsky, Michelle J.] NIH, Dept Bioeth, Ctr Clin, Bldg 10, Bethesda, MD 20892 USA.
[Berkman, Benjamin E.] NIH, Dept Bioeth, Bldg 10, Bethesda, MD 20892 USA.
[Berkman, Benjamin E.] NIH, Sect Eth Genet & Emerging Technol, Bldg 10, Bethesda, MD 20892 USA.
[Berkman, Benjamin E.] NHGRI, Bethesda, MD 20892 USA.
[Berkman, Benjamin E.] NHGRI, Bioeth Core, Bethesda, MD 20892 USA.
RP Bayefsky, MJ (reprint author), NIH, Dept Bioeth, Ctr Clin, Bldg 10, Bethesda, MD 20892 USA.
FU Intramural Research Program of the National Human Genome Research
Institute, National Institutes of Health
FX The opinions expressed herein are the authors' and do not reflect the
policies and positions of the National Institutes of Health, the U.S.
Public Health Service, or the U.S. Department of Health and Human
Services. This research was supported by the Intramural Research Program
of the National Human Genome Research Institute, National Institutes of
Health.
NR 48
TC 0
Z9 0
U1 2
U2 2
PU CAMBRIDGE UNIV PRESS
PI NEW YORK
PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA
SN 0963-1801
EI 1469-2147
J9 CAMB Q HEALTHC ETHIC
JI Camb. Q. Healthc. Ethics
PD JUL
PY 2016
VL 25
IS 3
BP 350
EP 365
DI 10.1017/S0963180115000687
PG 16
WC Health Care Sciences & Services; Health Policy & Services; Social
Sciences, Biomedical
SC Health Care Sciences & Services; Biomedical Social Sciences
GA DX5PA
UT WOS:000384432000002
PM 26864991
ER
PT J
AU Sera, Y
Yamasaki, N
Oda, H
Nagamachi, A
Wolff, L
Inukai, T
Inaba, T
Honda, H
AF Sera, Yasuyuki
Yamasaki, Norimasa
Oda, Hideaki
Nagamachi, Akiko
Wolff, Linda
Inukai, Takeshi
Inaba, Toshiya
Honda, Hiroaki
TI Identification of cooperative genes for E2A-PBX1 to develop acute
lymphoblastic leukemia
SO CANCER SCIENCE
LA English
DT Article
DE Acute lymphoblastic leukemia; conditional knock-in mice; E2A-PBX1;
retroviral insertional mutagenesis; Zfp521/ZNF521
ID ZINC-FINGER PROTEIN; ACUTE MYELOID-LEUKEMIA; T-CELL LYMPHOMAGENESIS;
B-LINEAGE LEUKEMIA; TRANSGENIC MICE; N-MYC; C-MYC; TRANSCRIPTION FACTOR;
PRE-B; EXPRESSION
AB E2A-PBX1 is a chimeric gene product detected in t(1;19)-bearing acute lymphoblastic leukemia (ALL) with B-cell lineage. To investigate the leukemogenic process, we generated conditional knock-in (cKI) mice for E2A-PBX1, in which E2A-PBX1 is inducibly expressed under the control of the endogenous E2A promoter. Despite the induced expression of E2A-PBX1, no hematopoietic disease was observed, strongly suggesting that additional genetic alterations are required to develop leukemia. To address this possibility, retroviral insertional mutagenesis was used. Virus infection efficiently induced T-cell, B-cell, and biphenotypic ALL in E2A-PBX1 cKI mice. Inverse PCR identified eight retroviral common integration sites, in which enhanced expression was observed in the Gfi1, Mycn, and Pim1 genes. In addition, it is of note that viral integration and overexpression of the Zfp521 gene was detected in one tumor with B-cell lineage; we previously identified Zfp521 as a cooperative gene with E2A-HLF, another E2A-involving fusion gene with B-lineage ALL. The cooperative oncogenicity of E2A-PBX1 with overexpressed Zfp521 in B-cell tumorigenesis was indicated by the finding that E2A-PBX1 cKI, Zfp521 transgenic compound mice developed B-lineage ALL. Moreover, upregulation of ZNF521, the human counterpart of Zfp521, was found in several human leukemic cell lines bearing t(1; 19). These results indicate that E2A-PBX1 cooperates with additional gene alterations to develop ALL. Among them, enhanced expression of ZNF521 may play a clinically relevant role in E2A fusion genes to develop B-lineage ALL.
C1 [Sera, Yasuyuki; Yamasaki, Norimasa; Honda, Hiroaki] Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Dis Model, Hiroshima, Japan.
[Oda, Hideaki] Tokyo Womens Med Univ, Dept Pathol, Tokyo, Japan.
[Nagamachi, Akiko; Inaba, Toshiya] Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Mol Oncol, Hiroshima, Japan.
[Wolff, Linda] NCI, Cellular Oncol Lab, Ctr Canc Res, Bethesda, MD 20892 USA.
[Inukai, Takeshi] Univ Yamanashi, Dept Pediat, Fac Med, Yamanashi, Japan.
RP Honda, H (reprint author), Hiroshima Univ, Res Inst Radiat Biol & Med, Dept Dis Model, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348553, Japan.
EM hhonda@hiroshima-u.ac.jp
FU Ministry of Education, Science and Culture of Japan
FX Ministry of Education, Science and Culture of Japan.
NR 45
TC 0
Z9 0
U1 1
U2 1
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1347-9032
EI 1349-7006
J9 CANCER SCI
JI Cancer Sci.
PD JUL
PY 2016
VL 107
IS 7
BP 890
EP 898
DI 10.1111/cas.12945
PG 9
WC Oncology
SC Oncology
GA DX9OD
UT WOS:000384724100004
PM 27088431
ER
PT J
AU Moses, HL
Roberts, AB
Derynck, R
AF Moses, Harold L.
Roberts, Anita B.
Derynck, Rik
TI The Discovery and Early Days of TGF-beta: A Historical Perspective
SO COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
LA English
DT Article
ID TRANSFORMING-GROWTH-FACTOR; SERINE THREONINE KINASE;
EPITHELIAL-MESENCHYMAL TRANSITION; MOLECULAR-WEIGHT COMPLEX; DAUER LARVA
DEVELOPMENT; HUMAN ENDOTHELIAL-CELLS; HUMAN-LUNG FIBROBLASTS;
AMINO-ACID-SEQUENCE; MAD-RELATED PROTEIN; II RECEPTOR
AB Transforming growth factors (TGFs) were discovered as activities that were secreted by cancer cells, and later by normal cells, and had the ability to phenotypically and reversibly transform immortalized fibroblasts. TGF-beta distinguished itself from TGF-alpha because it did not bind to the same epidermal growth factor (EGF) receptor as TGF-alpha and, therefore, acted through different cell-surface receptors and signaling mediators. This review summarizes the discovery of TGF-beta, the early developments in its molecular and biological characterization with its many biological activities in different cell and tissue contexts and its roles in disease, the realization that there is a family of secreted TGF-beta-related proteins with many differentiation functions in development and activities in normal cell and tissue physiology, and the subsequent identification and characterization of the receptors and effectors that mediate TGF-beta family signaling responses.
C1 [Moses, Harold L.] Vanderbilt Univ, Vanderbilt Ingram Canc Ctr, Nashville, TN 37232 USA.
[Roberts, Anita B.] NCI, Lab Cell Regulat & Carcinogenesis, Bethesda, MD 20892 USA.
[Derynck, Rik] Univ Calif San Francisco, Dept Cell & Tissue Biol, San Francisco, CA 94143 USA.
RP Derynck, R (reprint author), Univ Calif San Francisco, Dept Cell & Tissue Biol, San Francisco, CA 94143 USA.
EM rik.derynck@ucsf.edu
NR 219
TC 2
Z9 2
U1 3
U2 3
PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
PI COLD SPRING HARBOR
PA 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA
SN 1943-0264
J9 CSH PERSPECT BIOL
JI Cold Spring Harbor Perspect. Biol.
PD JUL
PY 2016
VL 8
IS 7
AR a021865
DI 10.1101/cshperspect.a021865
PG 26
WC Cell Biology
SC Cell Biology
GA DY1CE
UT WOS:000384831700006
ER
PT J
AU Lipsky, LM
Strawderman, MS
Olson, CM
AF Lipsky, Leah M.
Strawderman, Myla S.
Olson, Christine M.
TI Weight-related self-efficacy in relation to maternal body weight from
early pregnancy to 2 years post-partum
SO MATERNAL AND CHILD NUTRITION
LA English
DT Article
DE maternal weight change; weight-related behaviours; behaviour-specific
self-efficacy; prospective cohort
ID PSYCHOSOCIAL FACTORS; YOUNG-ADULTS; LIFE-STYLE; WOMEN; RETENTION; GAIN;
OBESITY; RISK; CHILDBEARING; ASSOCIATION
AB Excessive gestational weight gain may lead to long-term increases in maternal body weight and associated health risks. The purpose of this study was to examine the relationship between maternal body weight and weight-related self-efficacy from early pregnancy to 2 years post-partum. Women with live, singleton term infants from a population-based cohort study were included (n = 595). Healthy eating self-efficacy and weight control self-efficacy were assessed prenatally and at 1 year and 2 years post-partum. Body weight was measured at early pregnancy, before delivery, and 6 weeks, 1 year and 2 years post-partum. Behavioural (smoking, breastfeeding) and sociodemographic (age, education, marital status, income) covariates were assessed by medical record review and baseline questionnaires. Multi-level linear regression models were used to examine the longitudinal associations of self-efficacy measures with body weight. Approximately half of the sample (57%) returned to early pregnancy weight at some point by 2 years post-partum, and 9% became overweight or obese at 2 years post-partum. Body weight over time was inversely related to healthy eating (beta = -0.57, P = 0.02) and weight control (beta = -0.99, P < 0.001) self-efficacy in the model controlling for both self-efficacy measures as well as time and behavioural and sociodemographic covariates. Weight-related self-efficacy may be an important target for interventions to reduce excessive gestational weight gain and post-partum weight gain.
C1 [Lipsky, Leah M.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Hlth Behav Branch, Div Intramural Publ Hlth Res, Bethesda, MD USA.
[Strawderman, Myla S.; Olson, Christine M.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA.
RP Lipsky, LM (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Div Intramural Publ Hlth Res, 6100 Execut Blvd,Suite 7B13, Rockville, MD 20852 USA.
EM lipskylm@mail.nih.gov
OI Lipsky, Leah/0000-0003-2645-4388
FU NIH [HD29549]; Cornell University; Eunice Kennedy Shriver National
Institute of Child Health and Human Development
FX This research was funded by NIH grant #HD29549, Cornell University, and
the Intramural Research Program of the Eunice Kennedy Shriver National
Institute of Child Health and Human Development.
NR 46
TC 1
Z9 1
U1 2
U2 2
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1740-8695
EI 1740-8709
J9 MATERN CHILD NUTR
JI Matern. Child Nutr.
PD JUL
PY 2016
VL 13
IS 3
BP 569
EP 578
DI 10.1111/mcn.12149
PG 10
WC Nutrition & Dietetics; Pediatrics
SC Nutrition & Dietetics; Pediatrics
GA DX8PV
UT WOS:000384651900003
PM 25244078
ER
PT J
AU Narayanan, M
Martins, AJ
Tsang, JS
AF Narayanan, Manikandan
Martins, Andrew J.
Tsang, John S.
TI Robust Inference of Cell-to-Cell Expression Variations from Single-and
K-Cell Profiling
SO PLOS COMPUTATIONAL BIOLOGY
LA English
DT Article
ID RNA-SEQ; GENE-EXPRESSION; REGULATORY HETEROGENEITIES; NOISE;
TRANSCRIPTOMICS; CIRCUITS; TISSUES
AB Quantifying heterogeneity in gene expression among single cells can reveal information inaccessible to cell-population averaged measurements. However, the expression level of many genes in single cells fall below the detection limit of even the most sensitive technologies currently available. One proposed approach to overcome this challenge is to measure random pools of k cells (e.g., 10) to increase sensitivity, followed by computational "deconvolution" of cellular heterogeneity parameters (CHPs), such as the biological variance of single-cell expression levels. Existing approaches infer CHPs using either single-cell or k-cell data alone, and typically within a single population of cells. However, integrating both single-and k-cell data may reap additional benefits, and quantifying differences in CHPs across cell populations or conditions could reveal novel biological information. Here we present a Bayesian approach that can utilize single-cell, k-cell, or both simultaneously to infer CHPs within a single condition or their differences across two conditions. Using simulated as well as experimentally generated single-and k-cell data, we found situations where each data type would offer advantages, but using both together can improve precision and better reconcile CHP information contained in single-and k-cell data. We illustrate the utility of our approach by applying it to jointly generated single-and k-cell data to reveal CHP differences in several key inflammatory genes between resting and inflammatory cytokine-activated human macrophages, delineating differences in the distribution of 'ON' versus 'OFF' cells and in continuous variation of expression level among cells. Our approach thus offers a practical and robust framework to assess and compare cellular heterogeneity within and across biological conditions using modern multiplexed technologies.
C1 [Narayanan, Manikandan; Martins, Andrew J.; Tsang, John S.] NIAID, Syst Genom & Bioinformat Unit, Lab Syst Biol, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
RP Tsang, JS (reprint author), NIAID, Syst Genom & Bioinformat Unit, Lab Syst Biol, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
EM john.tsang@nih.gov
FU Intramural Research Program of NIAID, NIH
FX This work is supported by the Intramural Research Program of NIAID, NIH.
The funders had no role in study design, data collection and analysis,
decision to publish, or preparation of the manuscript.
NR 32
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PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-734X
EI 1553-7358
J9 PLOS COMPUT BIOL
JI PLoS Comput. Biol.
PD JUL
PY 2016
VL 12
IS 7
AR e1005016
DI 10.1371/journal.pcbi.1005016
PG 33
WC Biochemical Research Methods; Mathematical & Computational Biology
SC Biochemistry & Molecular Biology; Mathematical & Computational Biology
GA DW0SC
UT WOS:000383351400029
PM 27438699
ER
PT J
AU Nussinov, R
Papin, JA
AF Nussinov, Ruth
Papin, Jason A.
TI Computing Biology
SO PLOS COMPUTATIONAL BIOLOGY
LA English
DT Editorial Material
C1 [Nussinov, Ruth] NCI, Canc & Inflammat Program, Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Frederick, MD 21701 USA.
[Nussinov, Ruth] Tel Aviv Univ, Sackler Inst Mol Med, Sackler Sch Med, Dept Human Genet & Mol Med, Tel Aviv, Israel.
[Papin, Jason A.] Univ Virginia, Dept Biomed Engn, Charlottesville, VA USA.
RP Nussinov, R (reprint author), NCI, Canc & Inflammat Program, Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Frederick, MD 21701 USA.; Nussinov, R (reprint author), Tel Aviv Univ, Sackler Inst Mol Med, Sackler Sch Med, Dept Human Genet & Mol Med, Tel Aviv, Israel.
EM nussinor@helix.nih.gov; papin@virginia.edu
OI Papin, Jason/0000-0002-2769-5805
NR 0
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Z9 0
U1 0
U2 0
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-734X
EI 1553-7358
J9 PLOS COMPUT BIOL
JI PLoS Comput. Biol.
PD JUL
PY 2016
VL 12
IS 7
AR e1005050
DI 10.1371/journal.pcbi.1005050
PG 3
WC Biochemical Research Methods; Mathematical & Computational Biology
SC Biochemistry & Molecular Biology; Mathematical & Computational Biology
GA DW0SC
UT WOS:000383351400040
PM 27403659
ER
PT J
AU Subramanian, S
Krishna, MC
AF Subramanian, Sankaran
Krishna, Murali C.
TI Electron Paramagnetic Resonance Imaging 1. CW-EPR Imaging
SO RESONANCE-JOURNAL OF SCIENCE EDUCATION
LA English
DT Article
DE EPR; imaging; spatial encoding; back-projection; gradients;
spectral-spatial imaging; line width; relaxation; oxygen partial
pressure; spin probes; CW- and FT-EPR; rapid scan; rotating gradients;
redox status; oxidative stress; anti-oxidants; ischaemia
ID RAPID-SCAN
AB EPR (Electron Paramagnetic Resonance) imaging is particularly useful in monitoring hypoxic zones in tumors which are highly resistant to radiation and chemotherapeutic treatment. This first part of the article covers aspects of CW (continuous wave) imaging with details of FT (pulsed Fourier Transform)-EPR imaging covered in Part 2, to be published in the next issue of Resonance.
C1 [Subramanian, Sankaran] IIT Madras, Chem, Madras, Tamil Nadu, India.
[Krishna, Murali C.] NCI, Biophys Spect & Imaging Sect, NIH, Bethesda, MD 20892 USA.
RP Subramanian, S (reprint author), Indian Inst Technol, Madras 600036, Tamil Nadu, India.; Krishna, MC (reprint author), NCI, Radiat Biol Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
EM subukannan@gmail.com; cherukum@mail.nih.gov
NR 10
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Z9 0
U1 3
U2 3
PU INDIAN ACAD SCIENCES
PI BANGALORE
PA C V RAMAN AVENUE, SADASHIVANAGAR, P B #8005, BANGALORE 560 080, INDIA
SN 0971-8044
EI 0973-712X
J9 RESONANCE
JI Resonance
PD JUL
PY 2016
VL 21
IS 7
BP 597
EP 619
PG 23
WC Education, Scientific Disciplines
SC Education & Educational Research
GA DX7XP
UT WOS:000384603000003
ER
PT J
AU Tran, TM
Hong, S
Bennett, J
Colbert, RA
AF Tran, T. M.
Hong, S.
Bennett, J.
Colbert, R. A.
TI THE ROLE OF ERAP1 IN SPONDYLOARTHRITIS IN HLA-B27 TRANSGENIC RATS
SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
LA English
DT Meeting Abstract
C1 [Tran, T. M.; Hong, S.; Bennett, J.; Colbert, R. A.] NIAMSD, NIH, Bethesda, MD 20892 USA.
NR 0
TC 0
Z9 0
U1 4
U2 4
PU CLINICAL & EXPER RHEUMATOLOGY
PI PISA
PA VIA SANTA MARIA 31, 56126 PISA, ITALY
SN 0392-856X
EI 1593-098X
J9 CLIN EXP RHEUMATOL
JI Clin. Exp. Rheumatol.
PD JUL-AUG
PY 2016
VL 34
IS 4
MA INV19
BP 727
EP 728
PG 2
WC Rheumatology
SC Rheumatology
GA DW8TG
UT WOS:000383927800038
ER
PT J
AU Gensler, LS
Reveille, JD
Ward, MM
Lee, M
Learch, T
Brown, MA
Rahbar, MH
Weisman, MH
AF Gensler, L. S.
Reveille, J. D.
Ward, M. M.
Lee, M.
Learch, T.
Brown, M. A.
Rahbar, M. H.
Weisman, M. H.
TI HIGH DOSE NSAIDs AND TUMOR NECROSIS FACTOR INHIBITOR USE SYNERGIZE
TOWARDS LESS RADIOGRAPHIC PROGRESSION IN ANKYLOSING SPONDYLITIS - A
LONGITUDINAL ANALYSIS
SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
LA English
DT Meeting Abstract
C1 [Gensler, L. S.] Univ Calif San Francisco, Dept Med, San Francisco, CA USA.
[Reveille, J. D.; Lee, M.; Rahbar, M. H.] Univ Texas Hlth Sci Ctr Houston, Dept Med, Houston, TX 77030 USA.
[Ward, M. M.] Natl Inst Arthrit & Musculoskeletal & Skin Dis NI, NIH, Bethesda, MD USA.
[Learch, T.; Weisman, M. H.] Cedars Sinai Med Ctr, Dept Med, Los Angeles, CA 90048 USA.
[Brown, M. A.] Queensland Univ Technol, Brisbane, Qld, Australia.
NR 0
TC 0
Z9 0
U1 0
U2 0
PU CLINICAL & EXPER RHEUMATOLOGY
PI PISA
PA VIA SANTA MARIA 31, 56126 PISA, ITALY
SN 0392-856X
EI 1593-098X
J9 CLIN EXP RHEUMATOL
JI Clin. Exp. Rheumatol.
PD JUL-AUG
PY 2016
VL 34
IS 4
MA O9
BP 731
EP 732
PG 2
WC Rheumatology
SC Rheumatology
GA DW8TG
UT WOS:000383927800050
ER
PT J
AU Reveille, J
Ward, M
Lee, M
Weisman, M
Gensler, L
Rahbar, M
Brown, M
AF Reveille, J.
Ward, M.
Lee, M.
Weisman, M.
Gensler, L.
Rahbar, M.
Brown, M.
TI ETHNICITY AND DISEASE SEVERITY IN ANKYLOSING SPONDYLITIS
SO CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
LA English
DT Meeting Abstract
C1 [Reveille, J.; Lee, M.; Rahbar, M.] Univ Texas Houston, Houston, TX USA.
[Ward, M.] NIH, Ctr Clin, Bethesda, MD 20892 USA.
[Weisman, M.] Cedars Sinai, Los Angeles, CA USA.
[Gensler, L.] Univ Calif San Francisco, San Francisco, CA 94143 USA.
[Brown, M.] Queensland Univ Technol, Brisbane, Qld, Australia.
NR 0
TC 0
Z9 0
U1 0
U2 0
PU CLINICAL & EXPER RHEUMATOLOGY
PI PISA
PA VIA SANTA MARIA 31, 56126 PISA, ITALY
SN 0392-856X
EI 1593-098X
J9 CLIN EXP RHEUMATOL
JI Clin. Exp. Rheumatol.
PD JUL-AUG
PY 2016
VL 34
IS 4
MA P90
BP 763
EP 763
PG 1
WC Rheumatology
SC Rheumatology
GA DW8TG
UT WOS:000383927800143
ER
PT J
AU Jones-Lopez, EC
Acuna-Villaorduna, C
Ssebidandi, M
Gaeddert, M
Kubiak, RW
Ayakaka, I
White, LF
Joloba, M
Okwera, A
Fennelly, KP
AF Jones-Lopez, Edward C.
Acuna-Villaorduna, Carlos
Ssebidandi, Martin
Gaeddert, Mary
Kubiak, Rachel W.
Ayakaka, Irene
White, Laura F.
Joloba, Moses
Okwera, Alphonse
Fennelly, Kevin P.
TI Cough Aerosols of Mycobacterium tuberculosis in the Prediction of
Incident Tuberculosis Disease in Household Contacts
SO CLINICAL INFECTIOUS DISEASES
LA English
DT Article
DE Mycobacterium tuberculosis; cough-generated aerosols; inoculum;
predictors of disease progression; transmission
ID AIRBORNE TRANSMISSION; GROWTH-RATES; INFECTION; VARIABILITY; RISK;
RESPONSES
AB Background. Tuberculosis disease develops in only 5%-10% of humans infected with Mycobacterium tuberculosis. The mechanisms underlying this variability remain poorly understood. We recently demonstrated that colony-forming units of M. tuberculosis in cough-generated aerosols are a better predictor of infection than the standard sputum acid-fast bacilli smear. We hypothesized that cough aerosol cultures may also predict progression to tuberculosis disease in contacts.
Methods. We conducted a retrospective cohort study of 85 patients with smear-positive tuberculosis and their 369 household contacts in Kampala, Uganda. Index case patients underwent a standard evaluation, and we cultured M. tuberculosis from cough aerosols. Contacts underwent a standard evaluation at enrollment, and they were later traced to determine their tuberculosis status.
Results. During a median follow-up of 3.9 years, 8 (2%) of the contacts developed tuberculosis disease. In unadjusted and adjusted analyses, incident tuberculosis disease in contacts was associated with sputum Mycobacterial Growth Indicator Tube culture (odds ratio, 8.2; 95% confidence interval, 1.1-59.2; P=.04), exposure to a high-aerosol tuberculosis case patient (6.0, 1.4-25.2; P=.01), and marginally, human immunodeficiency virus in the contact (6.11; 0.89-41.7; P=.07). We present data demonstrating that sputum and aerosol specimens measure 2 related but different phenomena.
Conclusions. We found an increased risk of tuberculosis progression among contacts of high-aerosol case patients. The hypothesis that a larger infectious inoculum, represented by high aerosol production, determines the risk of disease progression deserves evaluation in future prospective studies.
C1 [Jones-Lopez, Edward C.; Acuna-Villaorduna, Carlos; Gaeddert, Mary; Kubiak, Rachel W.] Boston Med Ctr, Dept Med, Sect Infect Dis, Boston, MA USA.
[Jones-Lopez, Edward C.; Acuna-Villaorduna, Carlos; Gaeddert, Mary; Kubiak, Rachel W.] Boston Univ, Sch Med, Boston, MA 02118 USA.
[White, Laura F.] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA.
[Fennelly, Kevin P.] NHLBI, Pulm Clin Med Sect, Cardiovasc & Pulm Branch, Div Intramural Res,NIH, Bldg 10, Bethesda, MD 20892 USA.
[Jones-Lopez, Edward C.; Ssebidandi, Martin; Ayakaka, Irene; Okwera, Alphonse] Makerere Univ, Boston Med Ctr Res Collaborat, Kampala, Uganda.
[Joloba, Moses] Makerere Univ, Coll Hlth Sci, Dept Microbiol, Kampala, Uganda.
[Okwera, Alphonse] Mulago Hosp, Mulago Hosp, TB Clin, Kampala, Uganda.
RP Jones-Lopez, EC (reprint author), Boston Univ, Sch Med, Infect Dis Sect, 801 Massachusetts Ave,Crosstown Rm 2009, Boston, MA 02118 USA.; Jones-Lopez, EC (reprint author), Boston Med Ctr, 801 Massachusetts Ave,Crosstown Rm 2009, Boston, MA 02118 USA.
EM edward.jones@bmc.org
OI Joloba, Moses/0000-0002-0334-9983
FU Division of Global Medicine, Department of Medicine, University of
Florida; Section of Infectious Diseases, Department of Medicine, Boston
Medical Center and Boston University School of Medicine
FX This work was supported by personal funds from the Division of Global
Medicine, Department of Medicine, University of Florida, and from the
Section of Infectious Diseases, Department of Medicine, Boston Medical
Center and Boston University School of Medicine.
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PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1058-4838
EI 1537-6591
J9 CLIN INFECT DIS
JI Clin. Infect. Dis.
PD JUL 1
PY 2016
VL 63
IS 1
BP 10
EP 20
DI 10.1093/cid/ciw199
PG 11
WC Immunology; Infectious Diseases; Microbiology
SC Immunology; Infectious Diseases; Microbiology
GA DT1FH
UT WOS:000381226600007
PM 27025837
ER
PT J
AU Cohen, JI
Dropulic, L
Hsu, AP
Zerbe, CS
Krogmann, T
Dowdell, K
Hornung, RL
Lovell, J
Hardy, N
Hickstein, D
Cowen, EW
Calvo, KR
Pittaluga, S
Holland, SM
AF Cohen, Jeffrey I.
Dropulic, Lesia
Hsu, Amy P.
Zerbe, Christa S.
Krogmann, Tammy
Dowdell, Kennichi
Hornung, Ronald L.
Lovell, Jana
Hardy, Nancy
Hickstein, Dennis
Cowen, Edward W.
Calvo, Katherine R.
Pittaluga, Stefania
Holland, Steven M.
TI Association of GATA2 Deficiency With Severe Primary Epstein-Barr Virus
(EBV) Infection and EBV-associated Cancers
SO CLINICAL INFECTIOUS DISEASES
LA English
DT Article
DE GATA2; Epstein-Barr virus; hydroa vacciniforme; chronic active
Epstein-Barr disease; Epstein-Barr virus smooth muscle tumors
ID NATURAL-KILLER-CELLS; TRANSCRIPTION FACTOR GATA-2; ACUTE
MYELOID-LEUKEMIA; PRIMARY IMMUNODEFICIENCIES; SPORADIC MONOCYTOPENIA;
HUMAN CYTOMEGALOVIRUS; AUTOSOMAL-DOMINANT; MONOMAC SYNDROME; DENDRITIC
CELL; MUTATIONS
AB Background. Most patients infected with Epstein-Barr virus (EBV) are asymptomatic, have nonspecific symptoms, or have self-imiting infectious mononucleosis. EBV, however, may result in severe primary disease or cancer.
Methods. We report EBV diseases associated with GATA2 deficiency at one institution and describe the hematology, virology, and cytokine findings.
Results. Seven patients with GATA2 deficiency developed severe EBV disease. Three presented with EBV infectious mononucleosis requiring hospitalization, 1 had chronic active EBV disease (B-cell type), 1 had EBV-associated hydroa vacciniforme-like lymphoma with hemophagocytic lymphohistiocytosis, and 2 had EBV-positive smooth muscle tumors. Four of the 7 patients had severe warts and 3 had disseminated nontuberculous mycobacterial infections. All of the patients had low numbers of monocytes, B cells, CD4 T cells, and natural killer cells. All had elevated levels of EBV in the blood; 2 of 3 patients tested had expression of the EBV major immediate-early gene in the blood indicative of active EBV lytic infection. Mean plasma levels of tumor necrosis factor a, interferon., and interferon gamma-induced protein 10 were higher in patients with GATA2 deficiency than in controls.
Conclusions. GATA2 is the first gene associated with EBV hydroa vacciniforme-like lymphoma. GATA2 deficiency should be considered in patients with severe primary EBV infection or EBV-associated cancer, especially in those with disseminated nontuberculous mycobacterial disease and warts.
C1 [Cohen, Jeffrey I.; Dropulic, Lesia; Krogmann, Tammy; Dowdell, Kennichi] NIAID, Infect Dis Lab, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Hsu, Amy P.; Zerbe, Christa S.; Lovell, Jana; Holland, Steven M.] NIAID, Lab Clin Infect Dis, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Hardy, Nancy; Hickstein, Dennis] NCI, Expt Transplantat & Immunol Branch, Bethesda, MD 20892 USA.
[Cowen, Edward W.] NCI, Dermatol Branch, Ctr Canc Res, Bethesda, MD 20892 USA.
[Pittaluga, Stefania] NCI, Pathol Lab, Bethesda, MD 20892 USA.
[Calvo, Katherine R.] NIH, Ctr Clin, Dept Lab Med, Hematol Sect, Bldg 10, Bethesda, MD 20892 USA.
[Hornung, Ronald L.] Frederick Natl Lab Canc Res, Leidos Biomed Res, Clin Serv Program, Frederick, MD USA.
RP Cohen, JI (reprint author), NIH, Infect Dis Lab, Bldg 50,Rm 6134,50 S Dr,MSC8007, Bethesda, MD 20892 USA.
EM jcohen@niaid.nih.gov
FU National Institute of Allergy and Infectious Diseases; National Cancer
Institute; Clinical Center; National Cancer Institute, National
Institutes of Health [HHSN261200800001E]
FX This work was supported by the intramural research programs of the
National Institute of Allergy and Infectious Diseases, the National
Cancer Institute, and the Clinical Center. This project has been funded
in part with federal funds from the National Cancer Institute, National
Institutes of Health (contract HHSN261200800001E).
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PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1058-4838
EI 1537-6591
J9 CLIN INFECT DIS
JI Clin. Infect. Dis.
PD JUL 1
PY 2016
VL 63
IS 1
BP 41
EP 47
DI 10.1093/cid/ciw160
PG 7
WC Immunology; Infectious Diseases; Microbiology
SC Immunology; Infectious Diseases; Microbiology
GA DT1FH
UT WOS:000381226600011
PM 27169477
ER
PT J
AU Van Dyke, RB
Patel, K
Kagan, RM
Karalius, B
Traite, S
Meyer, WA
Tassiopoulos, KK
Seage, GR
Seybolt, LM
Burchett, S
Hazra, R
AF Van Dyke, Russell B.
Patel, Kunjal
Kagan, Ron M.
Karalius, Brad
Traite, Shirley
Meyer, William A., III
Tassiopoulos, Katherine K.
Seage, George R., III
Seybolt, Lorna M.
Burchett, Sandra
Hazra, Rohan
CA Pediat HIV AIDS Cohort Study PHACS
TI Antiretroviral Drug Resistance Among Children and Youth in the United
States With Perinatal HIV
SO CLINICAL INFECTIOUS DISEASES
LA English
DT Article
DE human immunodeficiency virus; antiviral resistance; children;
adolescents; perinatal infection
ID MEDICATION ADHERENCE; VIROLOGICAL FAILURE; INFECTION; MUTATIONS
AB Among 234 US youths with perinatal human immunodeficiency virus, 75% had antiretroviral resistance, substantially higher than that of the reference laboratory overall (36%-44%). Resistance to newer antiretrovirals and to all antiretrovirals in a class was uncommon. The only factor independently associated with future resistance was a higher peak viral load.
C1 [Van Dyke, Russell B.] Tulane Univ, Sch Med, Dept Pediat, 8408,1440 Canal St,Ste 1600, New Orleans, LA 70112 USA.
[Patel, Kunjal; Karalius, Brad; Tassiopoulos, Katherine K.; Seage, George R., III] Harvard TH Chan Sch Publ Hlth, Dept Epidemiol, Boston, MA USA.
[Kagan, Ron M.] Focus Diagnost, San Juan Capistrano, CA USA.
[Traite, Shirley] Harvard TH Chan Sch Publ Hlth, Ctr Biostat AIDS Res, Boston, MA USA.
[Meyer, William A., III] Quest Diagnost, Baltimore, MD USA.
[Seybolt, Lorna M.] Louisiana State Univ, Dept Pediat, Hlth Sci Ctr, New Orleans, LA USA.
[Burchett, Sandra] Harvard Med Sch, Dept Pediat, Boston, MA USA.
[Hazra, Rohan] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Bethesda, MD USA.
RP Van Dyke, RB (reprint author), Tulane Univ, Sch Med, Dept Pediat, 8408,1440 Canal St,Ste 1600, New Orleans, LA 70112 USA.
EM vandyke@tulane.edu
FU Eunice Kennedy Shriver National Institute of Child Health and Human
Development; National Institute on Drug Abuse; National Institute of
Allergy and Infectious Diseases; Office of AIDS Research; National
Institute of Mental Health; National Institute of Neurological Disorders
and Stroke; National Institute on Deafness and Other Communication
Disorders; National Heart, Lung, and Blood Institute; National Institute
of Dental and Craniofacial Research; National Institute on Alcohol Abuse
and Alcoholism; Harvard T.H. Chan School of Public Health [HD052102];
Tulane University School of Medicine [HD052104]
FX This work was supported by the Eunice Kennedy Shriver National Institute
of Child Health and Human Development with co-funding from the National
Institute on Drug Abuse, the National Institute of Allergy and
Infectious Diseases, the Office of AIDS Research, the National Institute
of Mental Health, the National Institute of Neurological Disorders and
Stroke, the National Institute on Deafness and Other Communication
Disorders, the National Heart, Lung, and Blood Institute, the National
Institute of Dental and Craniofacial Research, and the National
Institute on Alcohol Abuse and Alcoholism through cooperative agreements
with the Harvard T.H. Chan School of Public Health (HD052102) (Principal
Investigator [PI]: George Seage; Project Director: Julie Alperen) and
the Tulane University School of Medicine (HD052104) (PI: Russell Van
Dyke; Co-PI: Kenneth Rich; Project Director: Patrick Davis). Data
management services were provided by Frontier Science and Technology
Research Foundation (PI: Suzanne Siminski), and regulatory services and
logistical support were provided by Westat, Inc (PI: Julie Davidson).
NR 12
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PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1058-4838
EI 1537-6591
J9 CLIN INFECT DIS
JI Clin. Infect. Dis.
PD JUL 1
PY 2016
VL 63
IS 1
BP 133
EP 137
DI 10.1093/cid/ciw213
PG 5
WC Immunology; Infectious Diseases; Microbiology
SC Immunology; Infectious Diseases; Microbiology
GA DT1FH
UT WOS:000381226600024
PM 27056398
ER
PT J
AU Zhu, XG
Kim, DW
Zhao, L
Willingham, MC
Cheng, SY
AF Zhu, Xuguang
Kim, Dong Wook
Zhao, Li
Willingham, Mark C.
Cheng, Sheue-yann
TI SAHA-induced loss of tumor suppressor Pten gene promotes thyroid
carcinogenesis in a mouse model
SO ENDOCRINE-RELATED CANCER
LA English
DT Article
DE thyroid hormone receptors; tumorigenesis; genetically engineered mouse
model; thyroid cancer; vorinostat; SAHA; tumor suppressor PTEN
ID T-CELL LYMPHOMA; HISTONE DEACETYLASE INHIBITORS; HORMONE-BETA-RECEPTOR;
SUBEROYLANILIDE HYDROXAMIC ACID; CANCER CELLS; MOLECULAR-MECHANISMS;
SIGNALING PATHWAYS; RADIOIODINE UPTAKE; TARGETED MUTATION; SOLID TUMORS
AB Thyroid cancer is on the rise. Novel approaches are needed to improve the outcome of patients with recurrent and advanced metastatic thyroid cancers. FDA approval of suberoylanilide hydroxamic acid (SAHA; vorinostat), an inhibitor of histone deacetylase, for the treatment of hematological malignancies led to the clinical trials of vorinostat for advanced thyroid cancer. However, patients were resistant to vorinostat treatment. To understand the molecular basis of resistance, we tested the efficacy of SAHA in two mouse models of metastatic follicular thyroid cancer: Thrb(PV/PV) and Thrb(PV/PV)Pten(+/-) mice. In both, thyroid cancer is driven by overactivation of PI3K-AKT signaling. However, the latter exhibit more aggressive cancer progression due to haplodeficiency of the tumor suppressor, the Pten gene. SAHA had no effects on thyroid cancer progression in Thrb(PV/PV) mice, indicative of resistance to SAHA. Unexpectedly, thyroid cancer progressed in SAHA-treated Thrb(PV/PV)Pten(+/-) mice with accelerated occurrence of vascular invasion, anaplastic foci, and lung metastasis. Molecular analyses showed further activated PI3K-AKT in thyroid tumors of SAHA-treated Thrb(PV/PV)Pten(+/-) mice, resulting in the activated effectors, p-Rb, CDK6, p21(Cip1), p-cSrc, ezrin, and matrix metalloproteinases, to increase proliferation and invasion of tumor cells. Single-molecule DNA analysis indicated that the wild-type allele of the Pten gene was progressively lost, whereas carcinogenesis progressed in SAHA-treated Thrb(PV/PV)Pten(+/-) mice. Thus, this study has uncovered a novel mechanism by which SAHA-induced loss of the tumor suppressor Pten gene to promote thyroid cancer progression. Effectors downstream of the Pten loss-induced signaling may be potential targets to overcome resistance of thyroid cancer to SAHA.
C1 [Zhu, Xuguang; Kim, Dong Wook; Zhao, Li; Willingham, Mark C.; Cheng, Sheue-yann] NCI, Mol Biol Lab, Ctr Canc Res, NIH, Bldg 37, Bethesda, MD 20892 USA.
RP Cheng, SY (reprint author), NCI, Mol Biol Lab, Ctr Canc Res, NIH, Bldg 37, Bethesda, MD 20892 USA.
EM chengs@mail.nih.gov
FU Intramural Research Program of the Center for Cancer Research, National
Cancer Institute, National Institutes of Health
FX This research was supported by the Intramural Research Program of the
Center for Cancer Research, National Cancer Institute, National
Institutes of Health.
NR 43
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U2 1
PU BIOSCIENTIFICA LTD
PI BRISTOL
PA EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT,
ENGLAND
SN 1351-0088
EI 1479-6821
J9 ENDOCR-RELAT CANCER
JI Endocr.-Relat. Cancer
PD JUL
PY 2016
VL 23
IS 7
BP 521
EP 533
DI 10.1530/ERC-16-0103
PG 13
WC Oncology; Endocrinology & Metabolism
SC Oncology; Endocrinology & Metabolism
GA DX2HD
UT WOS:000384187900003
PM 27267120
ER
PT J
AU Alamri, AM
Kang, K
Groeneveld, S
Wang, WS
Zhong, XG
Kallakury, B
Hennighausen, L
Liu, XF
Furth, PA
AF Alamri, Ahmad M.
Kang, Keunsoo
Groeneveld, Svenja
Wang, Weisheng
Zhong, Xiaogang
Kallakury, Bhaskar
Hennighausen, Lothar
Liu, Xuefeng
Furth, Priscilla A.
TI Primary cancer cell culture: mammary-optimized vs conditional
reprogramming
SO ENDOCRINE-RELATED CANCER
LA English
DT Article
DE primary cell culture; mammary cancer; Brca1; genetically engineered
mouse models; transcriptome
ID EPITHELIAL-MESENCHYMAL TRANSITION; INTEGRATIVE GENOMICS VIEWER;
ESTROGEN-RECEPTOR-ALPHA; NEGATIVE BREAST-CANCER; STEM-CELLS; KERATIN
EXPRESSION; ROCK INHIBITOR; MOUSE MODEL; ER-ALPHA; GENE
AB The impact of different culture conditions on biology of primary cancer cells is not always addressed. Here, conditional reprogramming (CRC) was compared with mammary-optimized EpiCult-B (EpiC) for primary mammary epithelial cell isolation and propagation, allograft generation, and genome-wide transcriptional consequences using cancer and non-cancer mammary tissue from mice with different dosages of Brca1 and p53. Selective comparison to DMEM was included. Primary cultures were established with all three media, but CRC was most efficient for initial isolation (P < 0.05). Allograft development was faster using cells grown in EpiC compared with CRC (P < 0.05). Transcriptome comparison of paired CRC and EpiC cultures revealed 1700 differentially expressed genes by passage 20. CRC promoted Trp53 gene family upregulation and increased expression of epithelial differentiation genes, whereas EpiC elevated expression of epithelial-mesenchymal transition genes. Differences did not persist in allografts where both methods yielded allografts with relatively similar transcriptomes. Restricting passage (<7) reduced numbers of differentially expressed genes below 50. In conclusion, CRC was most efficient for initial cell isolation but EpiC was quicker for allograft generation. The extensive culture-specific gene expression patterns that emerged with longer passage could be limited by reducing passage number when both culture transcriptomes were equally similar to that of the primary tissue. Defining impact of culture condition and passage on the transcriptome of primary cells could assist experimental design and interpretation. For example, differences that appear with passage and culture condition are potentially exploitable for comparative studies targeting specific biological networks in different transcriptional environments.
C1 [Alamri, Ahmad M.; Groeneveld, Svenja; Wang, Weisheng; Furth, Priscilla A.] Georgetown Univ, Dept Oncol, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA.
[Alamri, Ahmad M.] King Khalid Univ, Dept Clin Lab Sci, Coll Appl Med Sci, Abha, Saudi Arabia.
[Kang, Keunsoo; Hennighausen, Lothar] Natl Inst Diabet & Digest & Kidney Dis, Lab Genet & Physiol, NIH, 8 Ctr Dr, Bethesda, MD USA.
[Kang, Keunsoo] Dankook Univ, Dept Microbiol, Cheonan, South Korea.
[Groeneveld, Svenja] Univ Munich, Dept Pharm, Munich, Germany.
[Zhong, Xiaogang] Georgetown Univ, Dept Biostat Bioinformat & Biomath, Washington, DC USA.
[Kallakury, Bhaskar; Liu, Xuefeng] Georgetown Univ, Dept Pathol, Lombardi Comprehens Canc Ctr, Washington, DC USA.
[Furth, Priscilla A.] Georgetown Univ, Dept Med, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA.
RP Furth, PA (reprint author), Georgetown Univ, Dept Oncol, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA.; Furth, PA (reprint author), Georgetown Univ, Dept Med, Lombardi Comprehens Canc Ctr, Washington, DC 20057 USA.
EM Paf3@georgetown.edu
FU NIH NCI [RO1 CA112176, 5P30CA051008]; Intramural Research Program of
NIDDK/NIH; King Khalid University, Abha, Saudi Arabia
FX Research performed here was supported by NIH NCI RO1 CA112176 (PAF), NIH
NCI 5P30CA051008 (Histology and Tissue, Genomics and Epigenomics, and
Animal Shared Resources), the Intramural Research Program of NIDDK/NIH,
and King Khalid University, Abha, Saudi Arabia (AMA).
NR 58
TC 0
Z9 0
U1 3
U2 3
PU BIOSCIENTIFICA LTD
PI BRISTOL
PA EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT,
ENGLAND
SN 1351-0088
EI 1479-6821
J9 ENDOCR-RELAT CANCER
JI Endocr.-Relat. Cancer
PD JUL
PY 2016
VL 23
IS 7
BP 535
EP 554
DI 10.1530/ERC-16-0071
PG 20
WC Oncology; Endocrinology & Metabolism
SC Oncology; Endocrinology & Metabolism
GA DX2HD
UT WOS:000384187900004
PM 27267121
ER
PT J
AU Gul, M
Katz, J
Chaudhry, AA
Hannah-Shmouni, F
Skarulis, M
Cochran, CS
AF Gul, Maryam
Katz, James
Chaudhry, Ammar A.
Hannah-Shmouni, Fady
Skarulis, Monica
Cochran, Craig S.
TI Clinical Images: Rheumatologic and imaging manifestations of thyroid
acropachy
SO ARTHRITIS & RHEUMATOLOGY
LA English
DT Editorial Material
C1 [Gul, Maryam; Katz, James; Hannah-Shmouni, Fady; Skarulis, Monica; Cochran, Craig S.] NIH, Bethesda, MD 20892 USA.
[Chaudhry, Ammar A.] Johns Hopkins Univ, Baltimore, MD USA.
RP Gul, M (reprint author), NIH, Bethesda, MD 20892 USA.
NR 0
TC 0
Z9 0
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2326-5191
EI 2326-5205
J9 ARTHRITIS RHEUMATOL
JI Arthritis Rheumatol.
PD JUL
PY 2016
VL 68
IS 7
BP 1636
EP 1636
DI 10.1002/art.39693
PG 1
WC Rheumatology
SC Rheumatology
GA DW2UO
UT WOS:000383497500010
PM 27014857
ER
PT J
AU Gupta, S
Tatouli, IP
Rosen, LB
Hasni, S
Alevizos, I
Manna, ZG
Rivera, J
Jiang, C
Siegel, RM
Holland, SM
Moutsopoulos, HM
Browne, SK
AF Gupta, Sarthak
Tatouli, Ioanna P.
Rosen, Lindsey B.
Hasni, Sarfaraz
Alevizos, Ilias
Manna, Zerai G.
Rivera, Juan
Jiang, Chao
Siegel, Richard M.
Holland, Steven M.
Moutsopoulos, Haralampos M.
Browne, Sarah K.
TI Distinct Functions of Autoantibodies Against Interferon in Systemic
Lupus Erythematosus A Comprehensive Analysis of Anticytokine
Autoantibodies in Common Rheumatic Diseases
SO ARTHRITIS & RHEUMATOLOGY
LA English
DT Article
ID PULMONARY ALVEOLAR PROTEINOSIS; COLONY-STIMULATING FACTOR; ANTI-CYTOKINE
AUTOANTIBODIES; IFN-GAMMA; MICROARRAY ANALYSIS; RITUXIMAB THERAPY;
REVISED CRITERIA; GENE-EXPRESSION; ALPHA; AUTOIMMUNITY
AB Objective. Anticytokine autoantibodies occur across a range of hematologic, pulmonary, and infectious diseases. However, systematic investigation of their presence and significance in autoimmune diseases is lacking. This study was undertaken to examine the distinct functions of anticytokine autoantibodies in patients with systemic lupus erythematosus (SLE) compared to patients with other rheumatic diseases and healthy controls.
Methods. Serum samples from patients with SLE (n=199), patients with primary Sjogren's syndrome (SS) (n=150), patients with rheumatoid arthritis (RA) (n5149), and healthy controls (n=200) were screened for 24 anticytokine autoantibodies using a multiplex bead-based assay. To evaluate the biologic activity of anticytokine autoantibodies, their ability to block cytokine-induced signal transduction or protein expression was measured. RNA sequencing was performed on whole blood in a subset of healthy controls and patients with SLE.
Results. Patients with SLE and those with SS had a striking excess of autoantibodies against interferons and the interferon-responsive chemokine interferon-inducible protein 10 (IP-10). Only autoantibodies against type I interferon, interleukin-12 (IL-12), and IL-22 exhibited neutralizing activity. In SLE, the presence of antiinterferon-gamma autoantibodies was correlated with more severe disease activity, higher levels of anti-doublestranded DNA antibodies, and elevated expression of interferon-a/b-inducible genes. Conversely, in SLE patients with blocking anti-interferon-a autoantibodies, the type I interferon gene expression signature was normalized. Anti-type III interferon autoantibodies (lambda(2), lambda(3)) and anti-IP-10 autoantibodies were newly recognized in SLE patient serum, and autoantibodies against macrophagecolony stimulating factor, IL-4, IL-7, IL-17, and IL-22, none of which have been previously identified in rheumatic conditions, were discovered.
Conclusion. Anticytokine autoantibodies are associated with distinct patterns of disease in SLE, SS, and RA. Anti-interferon autoantibodies are overrepresented in patients with SLE and those with SS, and fall into distinct functional classes, with only a subset of anti-type I interferon antibodies exhibiting neutralizing activity. Anti-interferon-gamma autoantibodies are correlated with increased disease activity and interferon-related gene expression, suggesting that such autoantibodies may contribute to the pathogenesis of SLE.
C1 [Gupta, Sarthak; Rosen, Lindsey B.; Holland, Steven M.; Browne, Sarah K.] NIAID, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Gupta, Sarthak; Siegel, Richard M.] NIAMSD, NIH, Bethesda, MD 20892 USA.
[Tatouli, Ioanna P.; Moutsopoulos, Haralampos M.] Natl Univ Athens, Athens, Greece.
[Rosen, Lindsey B.; Holland, Steven M.; Browne, Sarah K.] NIAID, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Hasni, Sarfaraz; Manna, Zerai G.; Rivera, Juan; Jiang, Chao] Natl Ctr Complementary & Integrat Hlth, NIH, Bethesda, MD USA.
[Alevizos, Ilias] Natl Inst Dent & Craniofacial Res, NIH, Bethesda, MD USA.
RP Holland, SM (reprint author), NIAID, Lab Clin Infect Dis, NIH, CRC B3-4141,MSC 1684, Bethesda, MD 20892 USA.; Moutsopoulos, HM (reprint author), Natl Univ Athens, Sch Med, Dept Pathophysiol, 75 M Asias St, Athens 11527, Greece.
EM smh@nih.gov; hmoutsop@med.uoa.gr
FU Divisions of the Intramural Research Program at the National Institute
of Allergy and Infectious Diseases; National Institute of Arthritis and
Musculoskeletal and Skin Diseases, NIH; Rheumatology Research Foundation
Scientist Development Award
FX Supported by the Divisions of the Intramural Research Program at the
National Institute of Allergy and Infectious Diseases and National
Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH. Dr.
Gupta is recipient of a Rheumatology Research Foundation Scientist
Development Award.
NR 49
TC 0
Z9 0
U1 2
U2 2
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2326-5191
EI 2326-5205
J9 ARTHRITIS RHEUMATOL
JI Arthritis Rheumatol.
PD JUL
PY 2016
VL 68
IS 7
BP 1677
EP 1687
DI 10.1002/art.39607
PG 11
WC Rheumatology
SC Rheumatology
GA DW2UO
UT WOS:000383497500015
PM 26815287
ER
PT J
AU Fradkin, JE
Hanlon, MC
Rodgers, GP
AF Fradkin, Judith E.
Hanlon, Mary C.
Rodgers, Griffin P.
TI NIH Precision Medicine Initiative: Implications for Diabetes Research
SO DIABETES CARE
LA English
DT Article
ID DISEASE
AB In his January 2015 State of the Union address, President Barack Obama announced a new Precision Medicine Initiative (PMI) to personalize approaches toward improving health and treating disease (www.whitehouse.gov/precisionmedicine). He stated that the goal of such an initiative was "to bring us closer to curing diseases like cancer and diabetes, and to give all of us access to the personalized information we need to keep ourselves and our families healthier." Since that time, the National Institutes of Health (NIH) has taken a leadership role in implementing the President's vision related to biomedical research (www.nih.gov/precisionmedicine). Here, we discuss the NIH component of the PMI, related ongoing diabetes research, and near-term research that could position the diabetes field to take full advantage of the opportunities that stem from the PMI.
C1 [Fradkin, Judith E.] NIDDK, Div Diabet Endocrinol & Metab Dis, NIH, Bethesda, MD 20892 USA.
[Hanlon, Mary C.] NIDDK, Off Sci Program & Policy Anal, NIH, Bethesda, MD 20892 USA.
[Rodgers, Griffin P.] NIDDK, NIH, Bethesda, MD 20892 USA.
RP Fradkin, JE (reprint author), NIDDK, Div Diabet Endocrinol & Metab Dis, NIH, Bethesda, MD 20892 USA.
EM fradkinj@mail.nih.gov
NR 20
TC 5
Z9 5
U1 2
U2 3
PU AMER DIABETES ASSOC
PI ALEXANDRIA
PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA
SN 0149-5992
EI 1935-5548
J9 DIABETES CARE
JI Diabetes Care
PD JUL
PY 2016
VL 39
IS 7
BP 1080
EP 1084
DI 10.2337/dc16-0541
PG 5
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA DT3YG
UT WOS:000381416500012
PM 27289128
ER
PT J
AU Chew, EY
Lovato, JF
Davis, MD
Gerstein, HC
Danis, RP
Ismail-Beigi, F
Genuth, S
Greven, CM
Perdue, LH
Cushman, WC
Elam, MB
Bigger, JT
Ginsberg, HN
Goff, DC
Ambrosius, WT
AF Chew, Emily Y.
Lovato, James F.
Davis, Matthew D.
Gerstein, Hertzel C.
Danis, Ronald P.
Ismail-Beigi, Faramarz
Genuth, Saul
Greven, Craig M.
Perdue, Letitia H.
Cushman, William C.
Elam, Marshall B.
Bigger, J. Thomas
Ginsberg, Henry N.
Goff, David C., Jr.
Ambrosius, Walter T.
CA Action Control Cardiovasc Risk
Action Control Cardiovasc Risk
TI Persistent Effects of Intensive Glycemic Control on Retinopathy in Type
2 Diabetes in the Action to Control Cardiovascular Risk in Diabetes
(ACCORD) Follow-On Study
SO DIABETES CARE
LA English
DT Article
ID THERAPY; FENOFIBRATE; PROGRESSION; TRIAL; MELLITUS; OUTCOMES
AB OBJECTIVES
This study investigated whether the beneficial effects of intensive glycemic control and fenofibrate treatment of dyslipidemia in reducing retinopathy progression demonstrated in the Action to Control Cardiovascular Risk in Diabetes (ACCORD) Eye Study persisted beyond the clinical trial.
RESEARCH DESIGN AND METHODS
The ACCORD Study (2003-2009) randomized participants with type 2 diabetes to intensive or standard treatment for glycemia (A1C level at <6.0% [42 mmol/mol] vs. 7.0-7.9% [53-63 mmol/mol]), systolic blood pressure (<120 vs. 140 mmHg), and dyslipidemia (fenofibrate [160 mg] plus simvastatin or placebo plus simvastatin). ACCORD Eye Study participants, who had baseline and year 4 eye examinations and fundus photographs, were reexamined in the ACCORD Follow-On (ACCORDION) Eye Study (2010-2014) 4 years after the ACCORD trial closeout. The outcome measure was diabetic retinopathy progression of three or more steps on the Early Treatment Diabetic Retinopathy Study scale.
RESULTS
Diabetic retinopathy progressed in 5.8% with intensive glycemic treatment versus 12.7% with standard (adjusted odds ratio [aOR] 0.42, 95% CI 0.28-0.63, P < 0.0001), 7.5% with intensive blood pressure treatment versus 6.0% for standard (aOR 1.21, 95% CI 0.61-2.40, P = 0.59), and 11.8% with fenofibrate versus 10.2% with placebo (aOR 1.13, 95% CI 0.71-1.79, P = 0.60) in ACCORDION Eye participants (n = 1,310).
CONCLUSIONS
Prior intensive glycemic control continued to reduce diabetic retinopathy progression, despite similar A1C levels, when the ACCORD Study ended. This is the first study in people with type 2 diabetes of 10 years' duration and established cardiovascular disease, unlike the newly diagnosed participants of the UK Prospective Diabetes Study, to demonstrate this effect. The benefit of fenofibrate, however, did not persist. Intensive blood pressure control had no effect.
C1 [Chew, Emily Y.] NEI, NIH, Bethesda, MD 20892 USA.
[Lovato, James F.; Greven, Craig M.; Perdue, Letitia H.; Ambrosius, Walter T.] Wake Forest Univ, Bowman Gray Sch Med, Winston Salem, NC USA.
[Davis, Matthew D.; Danis, Ronald P.] Univ Wisconsin, Madison, WI USA.
[Gerstein, Hertzel C.] McMaster Univ, Hamilton, ON, Canada.
[Gerstein, Hertzel C.] Hamilton Hlth Sci, Populat Hlth Res Inst, Hamilton, ON, Canada.
[Ismail-Beigi, Faramarz; Genuth, Saul] Case Western Reserve Univ, Cleveland, OH 44106 USA.
[Elam, Marshall B.] Memphis Vet Affairs Med Ctr, Memphis, TN USA.
[Bigger, J. Thomas; Ginsberg, Henry N.] Columbia Univ Coll Phys & Surg, 630 W 168th St, New York, NY 10032 USA.
[Goff, David C., Jr.] Colorado Sch Publ Hlth, Aurora, CO USA.
RP Chew, EY (reprint author), NEI, NIH, Bethesda, MD 20892 USA.
EM echew@nei.nih.gov
FU National Heart, Lung, and Blood Institute [N01-HC-95178, N01-HC-95179,
N01-HC-95180, N01-HC-95181, N01-HC-95182, N01-HC-95183, N01-HC-95184,
HHSN268201100027C]; IAA [Y1-HC-9035, Y1-HC-1010]; National Institutes of
Health; National Institute of Diabetes and Digestive and Kidney
Diseases; National Institute on Aging; National Eye Institute; Centers
for Disease Control and Prevention
FX ACCORD was supported by National Heart, Lung, and Blood Institute
contracts N01-HC-95178, N01-HC-95179, N01-HC-95180, N01-HC-95181,
N01-HC-95182, N01-HC-95183, N01-HC-95184, IAA #Y1-HC-9035, and
IAA#Y1-HC-1010. Other components of the National Institutes of Health,
including the National Institute of Diabetes and Digestive and Kidney
Diseases, the National Institute on Aging, and the National Eye
Institute, contributed funding. The Centers for Disease Control and
Prevention funded substudies within ACCORD on cost-effectiveness and
health-related quality of life. General Clinical Research Centers
provide support at many sites. ACCORDION activities were supported by
National Heart, Lung, and Blood Institute contract HHSN268201100027C.
NR 23
TC 0
Z9 0
U1 1
U2 1
PU AMER DIABETES ASSOC
PI ALEXANDRIA
PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA
SN 0149-5992
EI 1935-5548
J9 DIABETES CARE
JI Diabetes Care
PD JUL
PY 2016
VL 39
IS 7
BP 1089
EP 1100
DI 10.2337/dc16-0024
PG 12
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA DT3YG
UT WOS:000381416500014
ER
PT J
AU Purnell, JQ
Selzer, F
Wahed, AS
Pender, J
Pories, W
Pomp, A
Dakin, G
Mitchell, J
Garcia, L
Staten, MA
McCloskey, C
Cummings, DE
Flum, DR
Courcoulas, A
Wolfe, BM
AF Purnell, Jonathan Q.
Selzer, Faith
Wahed, Abdus S.
Pender, John
Pories, Walter
Pomp, Alfons
Dakin, Greg
Mitchell, James
Garcia, Luis
Staten, Myrlene A.
McCloskey, Carol
Cummings, David E.
Flum, David R.
Courcoulas, Anita
Wolfe, Bruce M.
TI Type 2 Diabetes Remission Rates After Laparoscopic Gastric Bypass and
Gastric Banding: Results of the Longitudinal Assessment of Bariatric
Surgery Study
SO DIABETES CARE
LA English
DT Article
ID MORBIDLY OBESE-PATIENTS; WEIGHT-LOSS; INSULIN SENSITIVITY; INCRETIN
LEVELS; BILE-ACIDS; LEPTIN; MELLITUS; GLUCOSE; METAANALYSIS; OUTCOMES
AB OBJECTIVE
The goals of this study were to determine baseline and postbariatric surgical characteristics associated with type 2 diabetes remission and if, after controlling for differences in weight loss, diabetes remission was greater after Roux-en-Y gastric bypass (RYGBP) than laparoscopic gastric banding (LAGB).
RESEARCH DESIGN AND METHODS
An observational cohort of obese participants was studied using generalized linear mixed models to examine the associations of bariatric surgery type and diabetes remission rates for up to 3 years. Of 2,458 obese participants enrolled, 1,868 (76%) had complete data to assess diabetes status at both baseline and at least one follow-up visit. Of these, 627 participants (34%) were classified with diabetes: 466 underwent RYGBP and 140 underwent LAGB.
RESULTS
After 3 years, 68.7% of RYGBP and 30.2% of LAGB participants were in diabetes remission. Baseline factors associated with diabetes remission included a lower weight for LAGB, greater fasting C-peptide, lower leptin-to-fat mass ratio for RYGBP, and a lower hemoglobin A(1c) without need for insulin for both procedures. After both procedures, greater postsurgical weight loss was associated with remission. However, even after controlling for differences in amount of weight lost, relative diabetes remission rates remained nearly twofold higher after RYGBP than LAGB.
CONCLUSIONS
Diabetes remission up to 3 years after RYGBP and LAGB was proportionally higher with increasing postsurgical weight loss. However, the nearly twofold greater weight loss-adjusted likelihood of diabetes remission in subjects undergoing RYGBP than LAGB suggests unique mechanisms contributing to improved glucose metabolism beyond weight loss after RYGBP.
C1 [Purnell, Jonathan Q.; Wolfe, Bruce M.] Oregon Hlth & Sci Univ, Dept Med, Portland, OR 97201 USA.
[Purnell, Jonathan Q.; Wolfe, Bruce M.] Oregon Hlth & Sci Univ, Dept Surg, Portland, OR 97201 USA.
[Selzer, Faith; Wahed, Abdus S.] Univ Pittsburgh, Grad Sch Publ Hlth, Pittsburgh, PA USA.
[Pender, John; Pories, Walter] East Carolina Univ, Brody Sch Med, Greenville, NC USA.
[Pomp, Alfons; Dakin, Greg] Weill Cornell Med Coll, New York, NY USA.
[Mitchell, James; Garcia, Luis] Neuropsychiat Res Inst, Grand Forks, ND USA.
[Mitchell, James; Garcia, Luis] Univ North Dakota, Sch Med & Hlth Sci, Grand Forks, ND USA.
[Staten, Myrlene A.] NIDDK, Bethesda, MD 20892 USA.
[McCloskey, Carol; Courcoulas, Anita] Univ Pittsburgh, Med Ctr, Dept Surg, Pittsburgh, PA USA.
[Cummings, David E.; Flum, David R.] Univ Washington, Dept Med, Seattle, WA USA.
[Cummings, David E.; Flum, David R.] Univ Washington, Dept Surg, Seattle, WA 98195 USA.
RP Purnell, JQ (reprint author), Oregon Hlth & Sci Univ, Dept Med, Portland, OR 97201 USA.; Purnell, JQ (reprint author), Oregon Hlth & Sci Univ, Dept Surg, Portland, OR 97201 USA.
EM purnellj@ohsu.edu
FU National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
[DCC-U01-DK066557]; Cornell University Medical Center Clinical
Translational Research Center (CTRC) [U01-DK66667, UL1-RR024996];
University of Washington [U01-DK66568]; CTRC [UL1-RR024153,
M01RR-00037]; Neuropsychiatric Research Institute [U01-DK66471]; East
Carolina University [U01-DK66526]; University of Pittsburgh Medical
Center [U01-DK66585]; Oregon Health & Science University [U01-DK66555]
FX This clinical study was a cooperative agreement funded by the National
Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
(DCC-U01-DK066557; Columbia, U01-DK66667 [in collaboration with Cornell
University Medical Center Clinical Translational Research Center (CTRC),
UL1-RR024996]; University of Washington, U01-DK66568 [in collaboration
with CTRC, M01RR-00037]; Neuropsychiatric Research Institute,
U01-DK66471; East Carolina University, U01-DK66526; University of
Pittsburgh Medical Center, U01-DK66585 [in collaboration with CTRC,
UL1-RR024153]; Oregon Health & Science University, U01-DK66555).
NR 32
TC 5
Z9 5
U1 0
U2 0
PU AMER DIABETES ASSOC
PI ALEXANDRIA
PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA
SN 0149-5992
EI 1935-5548
J9 DIABETES CARE
JI Diabetes Care
PD JUL
PY 2016
VL 39
IS 7
BP 1101
EP 1107
DI 10.2337/dc15-2138
PG 7
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA DT3YG
UT WOS:000381416500015
PM 27289123
ER
PT J
AU Cefalu, WT
Buse, JB
Tuomilehto, J
Fleming, GA
Ferrannini, E
Gerstein, HC
Bennett, PH
Ramachandran, A
Raz, I
Rosenstock, J
Kahn, SE
AF Cefalu, William T.
Buse, John B.
Tuomilehto, Jaakko
Fleming, G. Alexander
Ferrannini, Ele
Gerstein, Hertzel C.
Bennett, Peter H.
Ramachandran, Ambady
Raz, Itamar
Rosenstock, Julio
Kahn, Steven E.
TI Update and Next Steps for Real-World Translation of Interventions for
Type 2 Diabetes Prevention: Reflections From a Diabetes Care Editors'
Expert Forum
SO DIABETES CARE
LA English
DT Article
ID IMPAIRED GLUCOSE-TOLERANCE; LIFE-STYLE INTERVENTION;
RANDOMIZED-CONTROLLED-TRIAL; CORONARY-HEART-DISEASE; 10-YEAR FOLLOW-UP;
BETA-CELL FUNCTION; DA QING IGT; FASTING GLUCOSE; INSULIN-SECRETION;
PROGRAM OUTCOMES
AB The International Diabetes Federation estimates that 415 million adults worldwide now have diabetes and 318 million have impaired glucose tolerance. These numbers are expected to increase to 642 million and 482 million, respectively, by 2040. This burgeoning pandemic places an enormous burden on countries worldwide, particularly resource-poor regions. Numerous landmark trials evaluating both intensive lifestyle modification and pharmacological interventions have persuasively demonstrated that type 2 diabetes can be prevented or its onset can be delayed in high-risk individuals with impaired glucose tolerance. However, key challenges remain, including how to scale up such approaches for widespread translation and implementation, how to select appropriately from various interventions and tailor them for different populations and settings, and how to ensure that preventive interventions yield clinically meaningful, cost-effective outcomes. In June 2015, a Diabetes Care Editors' Expert Forum convened to discuss these issues. This article, an outgrowth of the forum, begins with a summary of seminal prevention trials, followed by a discussion of considerations for selecting appropriate populations for intervention and the clinical implications of the various diagnostic criteria for prediabetes. The authors outline knowledge gaps in need of elucidation and explore a possible new avenue for securing regulatory approval of a prevention-related indication for metformin, as well as specific considerations for future pharmacological interventions to delay the onset of type 2 diabetes. They conclude with descriptions of some innovative, pragmatic translational initiatives already under way around the world.
C1 [Cefalu, William T.] Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA.
[Buse, John B.] Univ N Carolina, Sch Med, Chapel Hill, NC USA.
[Tuomilehto, Jaakko] Natl Inst Hlth & Welf, Chron Dis Prevent Unit, Helsinki, Finland.
[Tuomilehto, Jaakko] Dasman Diabet Inst, Dasman, Kuwait.
[Tuomilehto, Jaakko] King Abdulaziz Univ, Saudi Diabet Res Grp, Jeddah, Saudi Arabia.
[Tuomilehto, Jaakko] Danube Univ Krems, Ctr Vasc Prevent, Krems, Austria.
[Fleming, G. Alexander] Kinexum, Harpers Ferry, WV USA.
[Ferrannini, Ele] CNR, Inst Clin Physiol, Pisa, Italy.
[Gerstein, Hertzel C.] McMaster Univ, Hamilton, ON, Canada.
[Gerstein, Hertzel C.] Hamilton Hlth Sci, Hamilton, ON, Canada.
[Bennett, Peter H.] NIH, Phoenix, AZ USA.
[Ramachandran, Ambady] India Diabet Res Fdn, Madras, Tamil Nadu, India.
[Bennett, Peter H.] Dr A Ramachandrans Diabet Hosp, Madras, Tamil Nadu, India.
[Raz, Itamar] Hadassah Hebrew Univ Hosp, Dept Internal Med, Diabet Unit, Jerusalem, Israel.
[Rosenstock, Julio] Med City, Dallas Diabet & Endocrine Ctr, Dallas, TX USA.
[Rosenstock, Julio] Univ Texas Southwestern Med Ctr, Dallas, TX USA.
[Kahn, Steven E.] VA Puget Sound Hlth Care Syst, Seattle, WA USA.
[Kahn, Steven E.] Univ Washington, Seattle, WA 98195 USA.
RP Cefalu, WT (reprint author), Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA.
EM william.cefalu@pbrc.edu
OI Kahn, Steven/0000-0001-7307-9002
FU National Institutes of Health (NIH) [1U54-GM-104940]; NIH
[P50-AT-002776]
FX Writing and editing support services for this article were provided by
Debbie Kendall of Kendall Editorial in Richmond, VA. The Editorial
Committee recognizes that the work of the journal and contributions such
as this Expert Forum would not be possible without the dedicated work
and continued support of many individuals. Specifically, the planning,
logistics, and funding of the meeting and the incredible editorial
support would not have been possible without the tireless efforts of
Chris Kohler and his staff at the ADA Publishing office. In addition,
the Editorial Committee thanks Lyn Reynolds and her staff in the ADA
editorial office for support and Anne Gooch at the Pennington Biomedical
Research Center for her valuable assistance in helping to organize the
Expert Forum. W.T.C. is supported in part by National Institutes of
Health (NIH) grant 1U54-GM-104940, which funds the Louisiana Clinical
and Translational Science Center, and NIH grant P50-AT-002776.
NR 138
TC 5
Z9 5
U1 2
U2 2
PU AMER DIABETES ASSOC
PI ALEXANDRIA
PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA
SN 0149-5992
EI 1935-5548
J9 DIABETES CARE
JI Diabetes Care
PD JUL
PY 2016
VL 39
IS 7
BP 1186
EP 1201
DI 10.2337/dc16-0873
PG 16
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA DT3YG
UT WOS:000381416500027
PM 27631469
ER
PT J
AU Hering, BJ
Clarke, WR
Bridges, ND
Eggerman, TL
Alejandro, R
Bellin, MD
Chaloner, K
Czarniecki, CW
Goldstein, JS
Hunsicker, LG
Kaufman, DB
Korsgren, O
Larsen, CP
Luo, XR
Markmann, JF
Naji, A
Oberholzer, J
Posselt, AM
Rickels, MR
Ricordi, C
Robien, MA
Senior, PA
Shapiro, AMJ
Stock, PG
Turgeon, NA
AF Hering, Bernhard J.
Clarke, William R.
Bridges, Nancy D.
Eggerman, Thomas L.
Alejandro, Rodolfo
Bellin, Melena D.
Chaloner, Kathryn
Czarniecki, Christine W.
Goldstein, Julia S.
Hunsicker, Lawrence G.
Kaufman, Dixon B.
Korsgren, Olle
Larsen, Christian P.
Luo, Xunrong
Markmann, James F.
Naji, Ali
Oberholzer, Jose
Posselt, Andrew M.
Rickels, Michael R.
Ricordi, Camillo
Robien, Mark A.
Senior, Peter A.
Shapiro, A. M. James
Stock, Peter G.
Turgeon, Nicole A.
CA Clinical Islet Transplantation Con
TI Phase 3 Trial of Transplantation of Human Islets in Type 1 Diabetes
Complicated by Severe Hypoglycemia
SO DIABETES CARE
LA English
DT Article
ID EXCHANGE CLINIC REGISTRY; INSULIN PUMP THERAPY; PANCREAS
TRANSPLANTATION; GLYCEMIC CONTROL; CURRENT STATE; AWARENESS; ADULTS;
MELLITUS; OUTCOMES; IDDM
AB OBJECTIVE
Impaired awareness of hypoglycemia (IAH) and severe hypoglycemic events (SHEs) cause substantial morbidity and mortality in patients with type 1 diabetes (T1D). Current therapies are effective in preventing SHEs in 50-80% of patients with IAH and SHEs, leaving a substantial number of patients at risk. We evaluated the effectiveness and safety of a standardized human pancreatic islet product in subjects in whom IAH and SHEs persisted despite medical treatment.
RESEARCH DESIGN AND METHODS
This multicenter, single-arm, phase 3 study of the investigational product purified human pancreatic islets (PHPI) was conducted at eight centers in North America. Forty-eight adults with T1D for >5 years, absent stimulated C-peptide, and documented IAH and SHEs despite expert care were enrolled. Each received immunosuppression and one or more transplants of PHPI, manufactured on-site under good manufacturing practice conditions using a common batch record and standardized lot release criteria and test methods. The primary end point was the achievement of HbA(1c) <7.0% (53 mmol/mol) at day 365 and freedom from SHEs from day 28 to day 365 after the first transplant.
RESULTS
The primary end point was successfully met by 87.5% of subjects at 1 year and by 71% at 2 years. The median HbA(1c) level was 5.6% (38 mmol/mol) at both 1 and 2 years. Hypoglycemia awareness was restored, with highly significant improvements in Clarke and HYPO scores (P > 0.0001). No study-related deaths or disabilities occurred. Five of the enrollees (10.4%) experienced bleeds requiring transfusions (corresponding to 5 of 75 procedures), and two enrollees (4.1%) had infections attributed to immunosuppression. Glomerular filtration rate decreased significantly on immunosuppression, and donor-specific antibodies developed in two patients.
CONCLUSIONS
Transplanted PHPI provided glycemic control, restoration of hypoglycemia awareness, and protection from SHEs in subjects with intractable IAH and SHEs. Safety events occurred related to the infusion procedure and immunosuppression, including bleeding and decreased renal function. Islet transplantation should be considered for patients with T1D and IAH in whom other, less invasive current treatments have been ineffective in preventing SHEs.
C1 [Hering, Bernhard J.; Bellin, Melena D.] Univ Minnesota, Schulze Diabet Inst, Minneapolis, MN 55455 USA.
[Hering, Bernhard J.] Univ Minnesota, Dept Surg, Box 242 UMHC, Minneapolis, MN 55455 USA.
[Clarke, William R.; Chaloner, Kathryn; Hunsicker, Lawrence G.] Univ Iowa, Clin Trials Stat & Data Management Ctr, Iowa City, IA USA.
[Bridges, Nancy D.; Czarniecki, Christine W.; Goldstein, Julia S.; Robien, Mark A.] NIAID, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Eggerman, Thomas L.] NIDDK, NIH, Bethesda, MD 20892 USA.
[Alejandro, Rodolfo; Ricordi, Camillo] Univ Miami, Miller Sch Med, Diabet Res Inst, Miami, FL 33136 USA.
[Bellin, Melena D.] Univ Minnesota, Dept Pediat, Minneapolis, MN 55455 USA.
[Kaufman, Dixon B.] Univ Wisconsin, Dept Surg, Div Transplantat, Madison, WI USA.
[Korsgren, Olle] Uppsala Univ, Dept Immunol Genet & Pathol, Uppsala, Sweden.
[Larsen, Christian P.; Turgeon, Nicole A.] Emory Univ, Emory Transplant Ctr, Atlanta, GA 30322 USA.
[Luo, Xunrong] Northwestern Univ, Feinberg Sch Med, Comprehens Transplant Ctr, Chicago, IL 60611 USA.
[Markmann, James F.] Harvard Med Sch, Massachusetts Gen Hosp, Div Transplant Surg, Boston, MA USA.
[Naji, Ali; Rickels, Michael R.] Univ Penn, Perelman Sch Med, Inst Diabet Obes & Metab, Philadelphia, PA 19104 USA.
[Naji, Ali; Rickels, Michael R.] Univ Penn, Perelman Sch Med, Dept Surg, Philadelphia, PA 19104 USA.
[Naji, Ali; Rickels, Michael R.] Univ Penn, Perelman Sch Med, Dept Med, Philadelphia, PA 19104 USA.
[Oberholzer, Jose] Univ Illinois Hosp & Hlth Sci Syst, Div Transplantat, Chicago, IL USA.
[Posselt, Andrew M.; Stock, Peter G.] Univ Calif San Francisco, Dept Surg, San Francisco, CA USA.
[Senior, Peter A.; Shapiro, A. M. James] Univ Alberta, Clin Islet Transplant Program, Edmonton, AB, Canada.
[Senior, Peter A.; Shapiro, A. M. James] Univ Alberta, Fac Med & Dent, Edmonton, AB, Canada.
RP Hering, BJ (reprint author), Univ Minnesota, Schulze Diabet Inst, Minneapolis, MN 55455 USA.; Hering, BJ (reprint author), Univ Minnesota, Dept Surg, Box 242 UMHC, Minneapolis, MN 55455 USA.
EM bhering@umn.edu
OI Ricordi, Camillo/0000-0001-8092-7153
FU National Institute of Allergy and Infectious Diseases; National
Institute for Diabetes and Digestive and Kidney Diseases [U01-AI-089317,
U01-AI-089316, U01-AI-065191, U01-DK-085531, 5U01-DK-070431-10,
U01-DK-070431, U01-DK070460, U01-AI-065193, U01-DK-070430,
U01-AI-065192]; General Clinical Research Center Awards and Clinical and
Translational Science Awards [UL1-TR-000454, 5UL1-RR-025741,
8UL1-TR-000150, UL1-TR-000004, UL1-TR-000050, 1UL1-TR-000460,
5M01-RR-000400, UL1-TR-000114, M01-RR-00040, UL1-TR-000003]; National
Institute of Allergy and Infectious Diseases of the National Institutes
of Health [NO1-AI-15416, UM1-AI-109565]
FX This research was supported by grants from the National Institute of
Allergy and Infectious Diseases and the National Institute for Diabetes
and Digestive and Kidney Diseases to the following institutions: Emory
University (Atlanta, GA), U01-AI-089317; Northwestern University
(Evanston, IL), U01-AI-089316; University of Alberta(Edmonton, Alberta,
Canada), U01-AI-065191; University of California, San Francisco (San
Francisco, CA) U01-DK-085531; University of Illinois, Chicago(Chicago,
IL), 5U01-DK-070431-10; University of Iowa (Iowa City, IA),
U01-DK-070431; University of Miami (Miami, FL), U01-DK070460; University
of Minnesota (Minneapolis, MN), U01-AI-065193; University of
Pennsylvania (Philadelphia, PA), U01-DK-070430; and Uppsala University
(Uppsala, Sweden), U01-AI-065192. In addition, the study was supported
by the following General Clinical Research Center Awards and Clinical
and Translational Science Awards: to Emory University, UL1-TR-000454;
Northwestern University, 5UL1-RR-025741 and 8UL1-TR-000150; University
of California, San Francisco, UL1-TR-000004; University of Illinois,
Chicago, UL1-TR-000050; University of Miami, 1UL1-TR-000460; University
of Minnesota, 5M01-RR-000400 and UL1-TR-000114; and University of
Pennsylvania, M01-RR-00040 and UL1-TR-000003. TrialShare, a public
database maintained by the Immune Tolerance Network, is supported by the
National Institute of Allergy and Infectious Diseases of the National
Institutes of Health under award numbers NO1-AI-15416 and UM1-AI-109565.
NR 40
TC 19
Z9 19
U1 3
U2 3
PU AMER DIABETES ASSOC
PI ALEXANDRIA
PA 1701 N BEAUREGARD ST, ALEXANDRIA, VA 22311-1717 USA
SN 0149-5992
EI 1935-5548
J9 DIABETES CARE
JI Diabetes Care
PD JUL
PY 2016
VL 39
IS 7
BP 1230
EP 1240
DI 10.2337/dc15-1988
PG 11
WC Endocrinology & Metabolism
SC Endocrinology & Metabolism
GA DT3YG
UT WOS:000381416500032
PM 27208344
ER
PT J
AU Yeung, EH
Sundaram, R
Bell, EM
Druschel, C
Kus, C
Xie, Y
Louis, GMB
AF Yeung, E. H.
Sundaram, R.
Bell, E. M.
Druschel, C.
Kus, C.
Xie, Y.
Louis, G. M. Buck
TI Infertility treatment and children's longitudinal growth between birth
and 3 years of age
SO HUMAN REPRODUCTION
LA English
DT Article
DE assisted reproductive technologies; infertility treatment; growth;
infant weight gain; ovulation induction
ID IN-VITRO FERTILIZATION; INTRACYTOPLASMIC SPERM INJECTION; ASSISTED
REPRODUCTIVE TECHNOLOGY; PHYSICAL HEALTH; UPSTATE KIDS; BORN; WEIGHT;
IVF; CHILDHOOD; FERTILITY
AB STUDY QUESTION: Does early childhood growth from birth through to 3 years of age differ by mode of conception?
SUMMARY ANSWER: Findings suggest early childhood growth was comparable for children irrespective of infertility treatment, but twins conceived with ovulation induction with or without intrauterine insemination (OI/IUI) were slightly smaller than twins conceived without treatment.
WHAT IS KNOWN ALREADY: Although studies have found that babies conceived with infertility treatment are born lighter and earlier than infants conceived without treatment, little research especially forn on-assisted reproductive technology (ART) treatments has focused on their continued growth during early childhood.
STUDY DESIGN, SIZE, DURATION: Upstate KIDS recruited infants born (2008-2010) to resident upstate New York mothers. Infants were sampled based on birth certificate indication of infertility treatment; specifically, for every singleton conceived by infertility treatment, three singletons without infertility treatment were recruited and matched on region of birth. All multiple births irrespective of treatment were also recruited. Children were prospectively followed, returning questionnaires every 4-6 months until 3 years of age. In total, 3905 singletons, 1129 sets of multiples (96% of whom were twins) enrolled into the study. Analyses included 3440 (88%) singletons (969 conceived with treatment; specifically, 433 with ART and 535 with OI/IUI) and 991 (88%) sets of multiples (439 conceived with treatment; specifically 233 with ART and 206 with OI/IUI) with growth data available.
PARTICIPANTS/MATERIALS, SETTING, METHODS: Mothers reported infertility treatment use at baseline and children's height and weight from pediatric visits. Self-reported use of ART was previously verified by linkage with the US Society for Assisted Reproductive Technology Clinic Outcome Reporting System (SART CORS) database. Mixed linear models with cubic splines accounting for age and age-gender interactions were used to estimate mean differences in growth from birth to 3 years by infertility treatment status and adjusting for maternal age, race, education, private insurance, smoking status during pregnancy, maternal pre-pregnancy and paternal body mass indices (BMI).
MAIN RESULTS AND THE ROLE OF CHANCE: Compared with singletons conceived without treatment (n = 2471), singletons conceived by infertility treatment (433 by assisted reproductive technologies(ART), 535 by OI/IUI and 1 unknown specific type) did not differ ingrowth. Compared with twins not conceived with treatment (n = 1076), twins conceived with OI/IUI (n = 368) weighed slightly less over follow-up (122 g). They were also proportionally smaller for their length (-0.17 weight-for-length z-score units). No differences in mean size over the 3 years were observed for twins conceived by ART, though some evidence of rapid weight gain from birth to 4 months (adjusted OR 1.08; 95% CI: 1.00-1.16) suggestive of catch up growth was observed.
LIMITATIONS, REASONS FOR CAUTION: Participants from upstate New York may not be representative of US infants. Although accounted for in statistical analysis, attrition during follow-up may have limited power to detect small differences.
WIDER IMPLICATIONS OF THE FINDINGS: This study is the first to prospectively track the growth of children conceived with and without infertility treatment in the USA, including a substantial number of twins. Our findings are similar to what was previously observed in the ART literature outside of the states.
STUDY FUNDING/COMPETING INTEREST(S): Supported by the Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD; contracts #HHSN275201200005C, #HHSN267200700019C). Authors have no competing interests to declare.
C1 [Yeung, E. H.; Sundaram, R.; Xie, Y.; Louis, G. M. Buck] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Div Intramural Populat Hlth Res, Rockville, MD 20852 USA.
[Bell, E. M.; Druschel, C.] SUNY Albany, Sch Publ Hlth, Dept Environm Hlth Sci, Albany, NY 12144 USA.
[Bell, E. M.] SUNY Albany, Dept Epidemiol & Biostat, Sch Publ Hlth, Albany, NY 12144 USA.
[Druschel, C.] New York State Dept Hlth, Ctr Environm Hlth, Bur Environm & Occupat Epidemiol, Albany, NY 12237 USA.
[Kus, C.] New York State Dept Hlth, Div Family Hlth, Albany, NY 12237 USA.
RP Yeung, EH (reprint author), 6710B Rockledge Dr,MSC7004, Bethesda, MD 20892 USA.
EM yeungedw@mail.nih.gov
OI Yeung, Edwina/0000-0002-3851-2613; Sundaram,
Rajeshwari/0000-0002-6918-5002
FU Eunice Kennedy Shriver National Institute of Child Health and Human
Development (NICHD) [HHSN275201200005C, HHSN267200700019C]
FX Supported by the Intramural Research Program of the Eunice Kennedy
Shriver National Institute of Child Health and Human Development (NICHD;
contracts #HHSN275201200005C, #HHSN267200700019C).
NR 48
TC 0
Z9 0
U1 2
U2 3
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0268-1161
EI 1460-2350
J9 HUM REPROD
JI Hum. Reprod.
PD JUL
PY 2016
VL 31
IS 7
BP 1621
EP 1628
DI 10.1093/humrep/dew106
PG 8
WC Obstetrics & Gynecology; Reproductive Biology
SC Obstetrics & Gynecology; Reproductive Biology
GA DV9EM
UT WOS:000383242900029
PM 27165624
ER
PT J
AU Chuang, LT
Randall, TC
Denny, L
Johnston, CM
Schmeler, KM
Covens, AL
Cibula, D
Bookman, MA
Rawal, S
DePetrillo, D
Nam, JH
Goodman, A
Naik, R
Manchanda, R
Gaffney, DK
Small, W
Creutzberg, C
Rattray, C
Kesic, V
Paraja, R
Eiken, M
Belleson, K
Coleman, RL
Barakat, RR
Trimble, EL
Quinn, M
AF Chuang, Linus T.
Randall, Thomas C.
Denny, Lynette
Johnston, Carolyn M.
Schmeler, Kathleen M.
Covens, Allan L.
Cibula, David
Bookman, Michael A.
Rawal, Sudhir
DePetrillo, Denny
Nam, Joo-Hyun
Goodman, Annekathryn
Naik, Raj
Manchanda, Ranjit
Gaffney, David K.
Small, William, Jr.
Creutzberg, Carien
Rattray, Carole
Kesic, Vesna
Paraja, Rene
Eiken, Mary
Belleson, Kristin
Coleman, Robert L.
Barakat, Richard R.
Trimble, Edward L.
Quinn, Michael
TI Sister Society Meeting on Global Education Development and Collaboration
Meeting Report
SO INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
LA English
DT Article
DE Sister society; Low- and middle-income countries; Global education;
Collaboration
ID GYNECOLOGIC ONCOLOGY; HEALTH; SURGERY
AB Objectives: To identify common barriers to teaching and training and to identify strategies that would be useful in developing future training programs in gynecologic oncology in low- and middle-income countries.
Methods: There is a lack of overall strategy to meet the needs of education and training in gynecologic oncology in low- and middle-income countries, the leaderships of sister societies and global health volunteers met at the European Society of Gynecologic Oncology in October 23, 2015. The challenges of the training programs supported by gynecologic oncology societies, major universities and individual efforts were presented and discussed. Strategies to improve education and training were identified.
Results: Major challenges include language barriers, limited surgical equipment, inadequate internet access, lack of local support for sustainability in training programs, inadequate pathology and radiation oncology, finance and a global deficiency in identifying sites and personnel in partnering or developing training programs. The leaderships identified various key components including consultation with the local Ministry of Health, local educational institutions; inclusion of the program into existing local programs, a needs assessment, and the development of curriculum and regional centers of excellence.
Conclusions: Proper preparation of training sites and trainers, the development of global curriculum, the establishment of centers of excellence, and the ability to measure outcomes are important to improve education and training in gynecologic oncology in low- and middle- income countries.
C1 [Chuang, Linus T.] Icahn Sch Med Mt Sinai, 1176 Fifth Ave,Box 1173, New York, NY 10029 USA.
[Randall, Thomas C.; Goodman, Annekathryn] Harvard Med Sch, Massachusetts Gen Hosp, Boston, MA USA.
[Denny, Lynette] Univ Cape Town, Schuur Hosp, ZA-7700 Rondebosch, South Africa.
[Johnston, Carolyn M.] Univ Michigan, Ann Arbor, MI 48109 USA.
[Schmeler, Kathleen M.; Coleman, Robert L.] Univ Texas MD Anderson Canc Ctr, Houston, TX 77030 USA.
[Covens, Allan L.] Univ Toronto, Odette Canc Ctr, Toronto, ON M5S 1A1, Canada.
[Cibula, David] Charles Univ Prague, Prague, Czech Republic.
[Cibula, David] Gen Univ Hosp, Prague, Czech Republic.
[Bookman, Michael A.] US Oncol Network, The Woodlands, TX USA.
[Rawal, Sudhir] Rajiv Gandhi Canc Inst, New Delhi, India.
[DePetrillo, Denny] Univ Toronto, CAREpath Canada, Toronto, ON M5S 1A1, Canada.
[Nam, Joo-Hyun] Univ Ulsan, Coll Med, Seoul, South Korea.
[Naik, Raj] Queen Elizabeth Hosp, Gateshead, England.
[Manchanda, Ranjit] Queen Mary Univ London, Barts Canc Inst, London, England.
[Gaffney, David K.] Univ Utah, Huntsman Canc Hosp, Salt Lake City, UT 84112 USA.
[Small, William, Jr.] Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USA.
[Creutzberg, Carien] Leiden Univ, Med Ctr, Leiden, Netherlands.
[Rattray, Carole] Univ West Indies, Mona Campus, Kingston, Jamaica.
[Kesic, Vesna] Clin Ctr Serbia, Belgrade, Serbia.
[Paraja, Rene] Inst Cancerol Amer, Antioquia, Colombia.
[Eiken, Mary] Int Gynecol Canc Soc, Louisville, KY USA.
[Belleson, Kristin] Soc Gynecol Oncol, Chicago, IL USA.
[Barakat, Richard R.] Mem Sloan Kettering Canc Ctr, 1275 York Ave, New York, NY 10021 USA.
[Trimble, Edward L.] NCI, Bethesda, MD 20892 USA.
[Quinn, Michael] Royal Womens Hosp, Melbourne, Vic, Australia.
RP Chuang, LT (reprint author), Icahn Sch Med Mt Sinai, 1176 Fifth Ave,Box 1173, New York, NY 10029 USA.
EM linus.chuang@mssm.edu
OI Cibula, David/0000-0001-6387-9356
FU NCI NIH HHS [P30 CA008748]
NR 6
TC 0
Z9 0
U1 2
U2 2
PU LIPPINCOTT WILLIAMS & WILKINS
PI PHILADELPHIA
PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA
SN 1048-891X
EI 1525-1438
J9 INT J GYNECOL CANCER
JI Int. J. Gynecol. Cancer
PD JUL
PY 2016
VL 26
IS 6
BP 1186
EP 1188
DI 10.1097/IGC.0000000000000735
PG 3
WC Oncology; Obstetrics & Gynecology
SC Oncology; Obstetrics & Gynecology
GA DW9CV
UT WOS:000383953500030
PM 27327155
ER
PT J
AU Singhal, A
Simmons, M
Lu, ZY
AF Singhal, Ayush
Simmons, Michael
Lu, Zhiyong
TI Text mining for precision medicine: automating disease-mutation
relationship extraction from biomedical literature
SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
LA English
DT Article
DE precision medicine; disease-mutation relationship; automated extraction;
machine learning; text mining; breast cancer; prostate cancer
ID SEQUENCE VARIANTS; SYSTEM; TOOL
AB Objective Identifying disease-mutation relationships is a significant challenge in the advancement of precision medicine. The aim of this work is to design a tool that automates the extraction of disease-related mutations from biomedical text to advance database curation for the support of precision medicine.
Materials and Methods We developed a machine-learning (ML) based method to automatically identify the mutations mentioned in the biomedical literature related to a particular disease. In order to predict a relationship between the mutation and the target disease, several features, such as statistical features, distance features, and sentiment features, were constructed. Our ML model was trained with a pre-labeled dataset consisting of manually curated information about mutation-disease associations. The model was subsequently used to extract disease-related mutations from larger biomedical literature corpora.
Results The performance of the proposed approach was assessed using a benchmarking dataset. Results show that our proposed approach gains significant improvement over the previous state of the art and obtains F-measures of 0.880 and 0.845 for prostate and breast cancer mutations, respectively.
Discussion To demonstrate its utility, we applied our approach to all abstracts in PubMed for 3 diseases (including a non-cancer disease). The mutations extracted were then manually validated against human-curated databases. The validation results show that the proposed approach is useful in a real-world setting to extract uncurated disease mutations from the biomedical literature.
Conclusions The proposed approach improves the state of the art for mutation-disease extraction from text. It is scalable and generalizable to identify mutations for any disease at a PubMed scale.
C1 [Singhal, Ayush; Simmons, Michael; Lu, Zhiyong] Natl Lib Med, NCBI, NIH, MSC3825,Bldg 38A,Rm 1003A,8600 Rockville Pike, Bethesda, MD 20894 USA.
RP Lu, ZY (reprint author), Natl Lib Med, NCBI, NIH, MSC3825,Bldg 38A,Rm 1003A,8600 Rockville Pike, Bethesda, MD 20894 USA.
EM zhiyong.lu@nih.gov
FU NIH Intramural Research Program; National Library of Medicine; NIH
Medical Research Scholars Program; NIH
FX This research was supported by the NIH Intramural Research Program,
National Library of Medicine, and the NIH Medical Research Scholars
Program, a public-private partnership supported jointly by the NIH and
generous contributions to the Foundation for the NIH from the Doris Duke
Charitable Foundation, the Howard Hughes Medical Institute, the American
Association for Dental Research, the Colgate-Palmolive Company, and
other private donors. No funds from the Doris Duke Charitable Foundation
were used to support research that used animals.
NR 26
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Z9 1
U1 8
U2 8
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 1067-5027
EI 1527-974X
J9 J AM MED INFORM ASSN
JI J. Am. Med. Inf. Assoc.
PD JUL
PY 2016
VL 23
IS 4
BP 766
EP 772
DI 10.1093/jamia/ocw041
PG 7
WC Computer Science, Information Systems; Computer Science,
Interdisciplinary Applications; Health Care Sciences & Services;
Information Science & Library Science; Medical Informatics
SC Computer Science; Health Care Sciences & Services; Information Science &
Library Science; Medical Informatics
GA DW6SS
UT WOS:000383782100013
PM 27121612
ER
PT J
AU Roberts, K
Demner-Fushman, D
AF Roberts, Kirk
Demner-Fushman, Dina
TI Interactive use of online health resources: a comparison of consumer and
professional questions
SO JOURNAL OF THE AMERICAN MEDICAL INFORMATICS ASSOCIATION
LA English
DT Article
DE consumer health informatics; online information seeking; consumer
language; question answering
ID INFORMATION-SEEKING; CLINICAL QUESTIONS; CARE; POINT
AB Objective To understand how consumer questions on online resources differ from questions asked by professionals, and how such consumer questions differ across resources.
Materials and Methods Ten online question corpora, 5 consumer and 5 professional, with a combined total of over 40 000 questions, were analyzed using a variety of natural language processing techniques. These techniques analyze questions at the lexical, syntactic, and semantic levels, exposing differences in both form and content.
Results Consumer questions tend to be longer than professional questions, more closely resemble open-domain language, and focus far more on medical problems. Consumers ask more sub-questions, provide far more background information, and ask different types of questions than professionals. Furthermore, there is substantial variance of these factors between the different consumer corpora.
Discussion The form of consumer questions is highly dependent upon the individual online resource, especially in the amount of background information provided. Professionals, on the other hand, provide very little background information and often ask much shorter questions. The content of consumer questions is also highly dependent upon the resource. While professional questions commonly discuss treatments and tests, consumer questions focus disproportionately on symptoms and diseases. Further, consumers place far more emphasis on certain types of health problems (eg, sexual health).
Conclusion Websites for consumers to submit health questions are a popular online resource filling important gaps in consumer health information. By analyzing how consumers write questions on these resources, we can better understand these gaps and create solutions for improving information access.
This article is part of the Special Focus on Person-Generated Health and Wellness Data, which published in the May 2016 issue, Volume 23, Issue 3.
C1 [Roberts, Kirk; Demner-Fushman, Dina] US Natl Lib Med, Lister Hill Natl Ctr Biomed Commun, NIH, 8600 Rockville Pike,Bldg 38A-1003H, Bethesda, MD 20894 USA.
RP Roberts, K (reprint author), US Natl Lib Med, Lister Hill Natl Ctr Biomed Commun, NIH, 8600 Rockville Pike,Bldg 38A-1003H, Bethesda, MD 20894 USA.
EM kirk.roberts@nih.gov
FU National Institutes of Health [1K99LM012104]; US National Library of
Medicine, National Institutes of Health
FX This work was supported by the National Institutes of Health
(1K99LM012104), as well as the intramural research program at the US
National Library of Medicine, National Institutes of Health.
NR 34
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U1 2
U2 2
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 1067-5027
EI 1527-974X
J9 J AM MED INFORM ASSN
JI J. Am. Med. Inf. Assoc.
PD JUL
PY 2016
VL 23
IS 4
BP 802
EP 811
DI 10.1093/jamia/ocw024
PG 10
WC Computer Science, Information Systems; Computer Science,
Interdisciplinary Applications; Health Care Sciences & Services;
Information Science & Library Science; Medical Informatics
SC Computer Science; Health Care Sciences & Services; Information Science &
Library Science; Medical Informatics
GA DW6SS
UT WOS:000383782100020
PM 27147494
ER
PT J
AU Cranfill, PJ
Sell, BR
Baird, MA
Allen, JR
Lavagnino, Z
de Gruiter, HM
Kremers, GJ
Davidson, MW
Ustione, A
Piston, DW
AF Cranfill, Paula J.
Sell, Brittney R.
Baird, Michelle A.
Allen, John R.
Lavagnino, Zeno
de Gruiter, H. Martijn
Kremers, Gert-Jan
Davidson, Michael W.
Ustione, Alessandro
Piston, David W.
TI Quantitative assessment of fluorescent proteins
SO NATURE METHODS
LA English
DT Article
ID MONOMERIC RED; IN-VIVO; GREEN; MICROSCOPY; BRIGHT; GFP; ORANGE; FRET;
EVOLUTION; VARIANTS
AB The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight beta-barrel structure, which provides the rigidity and chemical environment needed for effectual fluorescence. Despite the common structure, each FP has unique properties. Thus, there is no single 'best' FP for every circumstance, and each FP has advantages and disadvantages. To guide decisions about which FP is right for a given application, we have quantitatively characterized the brightness, photostability, pH stability and monomeric properties of more than 40 FPs to enable straightforward and direct comparison between them. We focus on popular and/or top-performing FPs in each spectral region.
C1 [Cranfill, Paula J.; Sell, Brittney R.; Baird, Michelle A.; Allen, John R.; Davidson, Michael W.] Florida State Univ, Natl High Field Magnet Lab, Tallahassee, FL 32306 USA.
[Cranfill, Paula J.; Lavagnino, Zeno; Ustione, Alessandro; Piston, David W.] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA.
[Lavagnino, Zeno; Ustione, Alessandro; Piston, David W.] Washington Univ, Dept Cell Biol & Physiol, St Louis, MO USA.
[de Gruiter, H. Martijn; Kremers, Gert-Jan] Erasmus MC, Josephine Nefkens Inst, Erasmus Opt Imaging Ctr, Rotterdam, Netherlands.
[Cranfill, Paula J.] H Lee Moffitt Canc Ctr & Res Inst, Tampa, FL USA.
[Sell, Brittney R.] George Washington Univ, Dept Biochem & Mol Med, Washington, DC USA.
[Baird, Michelle A.] NHLBI, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Allen, John R.] Nikon Instruments, Melville, NY USA.
RP Piston, DW (reprint author), Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA.; Piston, DW (reprint author), Washington Univ, Dept Cell Biol & Physiol, St Louis, MO USA.
EM piston@wustl.edu
OI Lavagnino, Zeno/0000-0002-7247-8084
FU NIH [R01DK085064, R01DK098659, S100D010681, P20GM072048]
FX This work was supported in part by NIH grants R01DK085064, R01DK098659,
S10OD010681 and P20GM072048 to D.W.P.
NR 65
TC 20
Z9 20
U1 18
U2 21
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 1548-7091
EI 1548-7105
J9 NAT METHODS
JI Nat. Methods
PD JUL
PY 2016
VL 13
IS 7
BP 557
EP +
DI 10.1038/NMETH.3891
PG 7
WC Biochemical Research Methods
SC Biochemistry & Molecular Biology
GA DW6XK
UT WOS:000383794500012
PM 27240257
ER
PT J
AU Essa, MM
Akbar, M
Khan, MAS
AF Essa, Musthafa Mohamed
Akbar, Mohammed
Khan, Mohammed Abdul Sattar
TI Beneficial effects of date palm fruits on neurodegenerative diseases
SO NEURAL REGENERATION RESEARCH
LA English
DT Editorial Material
ID DIETARY SUPPLEMENTATION; ANTIOXIDANT ACTIVITY; ALZHEIMERS-DISEASE;
AMYLOID-BETA
C1 [Essa, Musthafa Mohamed] Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Food Sci & Nutr, Muscat, Oman.
[Essa, Musthafa Mohamed] Sultan Qaboos Univ, Ageing & Dementia Res Grp, Muscat, Oman.
[Akbar, Mohammed] NIAAA, Sect Mol Pharmacol & Toxicol, Lab Membrane Biochem & Biophys, NIH, Bethesda, MD USA.
[Khan, Mohammed Abdul Sattar] Massachusetts Gen Hosp, Shriners Hosp Children, Dept Anesthesia Crit Care & Pain Med, Boston, MA 02114 USA.
[Khan, Mohammed Abdul Sattar] Harvard Med Sch, Boston, MA USA.
RP Essa, MM (reprint author), Sultan Qaboos Univ, Coll Agr & Marine Sci, Dept Food Sci & Nutr, Muscat, Oman.; Essa, MM (reprint author), Sultan Qaboos Univ, Ageing & Dementia Res Grp, Muscat, Oman.
EM drmdessa@gmail.com
NR 15
TC 0
Z9 0
U1 2
U2 2
PU MEDKNOW PUBLICATIONS & MEDIA PVT LTD
PI MUMBAI
PA B-9, KANARA BUSINESS CENTRE, OFF LINK RD, GHAKTOPAR-E, MUMBAI, 400075,
INDIA
SN 1673-5374
EI 1876-7958
J9 NEURAL REGEN RES
JI Neural Regen. Res.
PD JUL
PY 2016
VL 11
IS 7
BP 1071
EP 1072
DI 10.4103/1673-5374.187032
PG 2
WC Cell Biology; Neurosciences
SC Cell Biology; Neurosciences & Neurology
GA DW6YW
UT WOS:000383798300015
PM 27630684
ER
PT J
AU Van Voorhis, WC
Adams, JH
Adelfio, R
Ahyong, V
Akabas, MH
Alano, P
Alday, A
Resto, YA
Alsibaee, A
Alzualde, A
Andrews, KT
Avery, SV
Avery, VM
Ayong, L
Baker, M
Baker, S
Ben Mamoun, C
Bhatia, S
Bickle, Q
Bounaadja, L
Bowling, T
Bosch, J
Boucher, LE
Boyom, FF
Brea, J
Brennan, M
Burton, A
Caffrey, CR
Camarda, G
Carrasquilla, M
Carter, D
Cassera, MB
Cheng, KCC
Chindaudomsate, W
Chubb, A
Colon, BL
Colon-Lopez, DD
Corbett, Y
Crowther, GJ
Cowan, N
D'Alessandro, S
Le Dang, N
Delves, M
DeRisi, JL
Du, AY
Duffy, S
El-Sayed, SA
Ferdig, MT
Robledo, JAF
Fidock, DA
Florent, I
Fokou, PVT
Galstian, A
Gamo, FJ
Gokool, S
Gold, B
Golub, T
Goldgof, GM
Guha, R
Guiguemde, WA
Gural, N
Guy, RK
Hansen, MAE
Hanson, KK
Hemphill, A
van Huijsduijnen, RH
Horii, T
Horrocks, P
Hughes, TB
Huston, C
Igarashi, I
Ingram-Sieber, K
Itoe, MA
Jadhav, A
Jensen, AN
Jensen, LT
Jiang, RHY
Kaiser, A
Keiser, J
Ketas, T
Kicka, S
Kim, S
Kirk, K
Kumar, VP
Kyle, DE
Lafuente, MJ
Landfear, S
Lee, N
Lee, S
Lehane, AM
Li, FW
Little, D
Liu, LQ
Llinas, M
Loza, MI
Lubar, A
Lucantoni, L
Lucet, I
Maes, L
Mancama, D
Mansour, NR
March, S
McGowan, S
Vera, IM
Meister, S
Mercer, L
Mestres, J
Mfopa, AN
Misra, RN
Moon, S
Moore, JP
da Costa, FMR
Muller, J
Muriana, A
Hewitt, SN
Nare, B
Nathan, C
Narraidoo, N
Nawaratna, S
Ojo, KK
Ortiz, D
Panic, G
Papadatos, G
Parapini, S
Patra, K
Pham, N
Prats, S
Plouffe, DM
Poulsen, SA
Pradhan, A
Quevedo, C
Quinn, RJ
Rice, CA
Rizk, MA
Ruecker, A
St Onge, R
Ferreira, RS
Samra, J
Robinett, NG
Schlecht, U
Schmitt, M
Villela, FS
Silvestrini, F
Sinden, R
Smith, DA
Soldati, T
Spitzmuller, A
Stamm, SM
Sullivan, DJ
Sullivan, W
Suresh, S
Suzuki, BM
Suzuki, Y
Swamidass, SJ
Taramelli, D
Tchokouaha, LRY
Theron, A
Thomas, D
Tonissen, KF
Townson, S
Tripathi, AK
Trofimov, V
Udenze, KO
Ullah, I
Vallieres, C
Vigil, E
Vinetz, JM
Vinh, PV
Vu, H
Watanabe, NA
Weatherby, K
White, PM
Wilks, AF
Winzeler, EA
Wojcik, E
Wree, M
Wu, W
Yokoyama, N
Zollo, PHA
Abla, N
Blasco, B
Burrows, J
Laleu, B
Leroy, D
Spangenberg, T
Wells, T
Willis, PA
AF Van Voorhis, Wesley C.
Adams, John H.
Adelfio, Roberto
Ahyong, Vida
Akabas, Myles H.
Alano, Pietro
Alday, Aintzane
Resto, Yesmalie Aleman
Alsibaee, Aishah
Alzualde, Ainhoa
Andrews, Katherine T.
Avery, Simon V.
Avery, Vicky M.
Ayong, Lawrence
Baker, Mark
Baker, Stephen
Ben Mamoun, Choukri
Bhatia, Sangeeta
Bickle, Quentin
Bounaadja, Lotfi
Bowling, Tana
Bosch, Juergen
Boucher, Lauren E.
Boyom, Fabrice F.
Brea, Jose
Brennan, Marian
Burton, Audrey
Caffrey, Conor R.
Camarda, Grazia
Carrasquilla, Manuela
Carter, Dee
Cassera, Maria Belen
Cheng, Ken Chih-Chien
Chindaudomsate, Worathad
Chubb, Anthony
Colon, Beatrice L.
Colon-Lopez, Daisy D.
Corbett, Yolanda
Crowther, Gregory J.
Cowan, Noemi
D'Alessandro, Sarah
Le Dang, Na
Delves, Michael
DeRisi, Joseph L.
Du, Alan Y.
Duffy, Sandra
El-Sayed, Shimaa Abd El-Salam
Ferdig, Michael T.
Robledo, Jose A. Fernandez
Fidock, David A.
Florent, Isabelle
Fokou, Patrick V. T.
Galstian, Ani
Javier Gamo, Francisco
Gokool, Suzanne
Gold, Ben
Golub, Todd
Goldgof, Gregory M.
Guha, Rajarshi
Guiguemde, W. Armand
Gural, Nil
Guy, R. Kiplin
Hansen, Michael A. E.
Hanson, Kirsten K.
Hemphill, Andrew
van Huijsduijnen, Rob Hooft
Horii, Takaaki
Horrocks, Paul
Hughes, Tyler B.
Huston, Christopher
Igarashi, Ikuo
Ingram-Sieber, Katrin
Itoe, Maurice A.
Jadhav, Ajit
Jensen, Amornrat Naranuntarat
Jensen, Laran T.
Jiang, Rays H. Y.
Kaiser, Annette
Keiser, Jennifer
Ketas, Thomas
Kicka, Sebastien
Kim, Sunyoung
Kirk, Kiaran
Kumar, Vidya P.
Kyle, Dennis E.
Jose Lafuente, Maria
Landfear, Scott
Lee, Nathan
Lee, Sukjun
Lehane, Adele M.
Li, Fengwu
Little, David
Liu, Liqiong
Llinas, Manuel
Loza, Maria I.
Lubar, Aristea
Lucantoni, Leonardo
Lucet, Isabelle
Maes, Louis
Mancama, Dalu
Mansour, Nuha R.
March, Sandra
McGowan, Sheena
Vera, Iset Medina
Meister, Stephan
Mercer, Luke
Mestres, Jordi
Mfopa, Alvine N.
Misra, Raj N.
Moon, Seunghyun
Moore, John P.
Rodrigues da Costa, Francielly Morais
Mueller, Joachim
Muriana, Arantza
Hewitt, Stephen Nakazawa
Nare, Bakela
Nathan, Carl
Narraidoo, Nathalie
Nawaratna, Sujeevi
Ojo, Kayode K.
Ortiz, Diana
Panic, Gordana
Papadatos, George
Parapini, Silvia
Patra, Kailash
Ngoc Pham
Prats, Sarah
Plouffe, David M.
Poulsen, Sally-Ann
Pradhan, Anupam
Quevedo, Celia
Quinn, Ronald J.
Rice, Christopher A.
Rizk, Mohamed Abdo
Ruecker, Andrea
St Onge, Robert
Ferreira, Rafaela Salgado
Samra, Jasmeet
Robinett, Natalie G.
Schlecht, Ulrich
Schmitt, Marjorie
Villela, Filipe Silva
Silvestrini, Francesco
Sinden, Robert
Smith, Dennis A.
Soldati, Thierry
Spitzmueller, Andreas
Stamm, Serge Maximilian
Sullivan, David J.
Sullivan, William
Suresh, Sundari
Suzuki, Brian M.
Suzuki, Yo
Swamidass, S. Joshua
Taramelli, Donatella
Tchokouaha, Lauve R. Y.
Theron, Anjo
Thomas, David
Tonissen, Kathryn F.
Townson, Simon
Tripathi, Abhai K.
Trofimov, Valentin
Udenze, Kenneth O.
Ullah, Imran
Vallieres, Cindy
Vigil, Edgar
Vinetz, Joseph M.
Phat Voong Vinh
Hoan Vu
Watanabe, Nao-aki
Weatherby, Kate
White, Pamela M.
Wilks, Andrew F.
Winzeler, Elizabeth A.
Wojcik, Edward
Wree, Melanie
Wu, Wesley
Yokoyama, Naoaki
Zollo, Paul H. A.
Abla, Nada
Blasco, Benjamin
Burrows, Jeremy
Laleu, Benoit
Leroy, Didier
Spangenberg, Thomas
Wells, Timothy
Willis, Paul A.
TI Open Source Drug Discovery with the Malaria Box Compound Collection for
Neglected Diseases and Beyond
SO PLOS PATHOGENS
LA English
DT Article
ID STAGE PLASMODIUM-FALCIPARUM; IN-VITRO; INFECTED ERYTHROCYTES; THEILERIA
PARASITES; IDENTIFICATION; INHIBITORS; TRANSMISSION; TARGET; BABESIA;
BLOOD
AB A major cause of the paucity of new starting points for drug discovery is the lack of interaction between academia and industry. Much of the global resource in biology is present in universities, whereas the focus of medicinal chemistry is still largely within industry. Open source drug discovery, with sharing of information, is clearly a first step towards overcoming this gap. But the interface could especially be bridged through a scale-up of open sharing of physical compounds, which would accelerate the finding of new starting points for drug discovery. The Medicines for Malaria Venture Malaria Box is a collection of over 400 compounds representing families of structures identified in phenotypic screens of pharmaceutical and academic libraries against the Plasmodium falciparum malaria parasite. The set has now been distributed to almost 200 research groups globally in the last two years, with the only stipulation that information from the screens is deposited in the public domain. This paper reports for the first time on 236 screens that have been carried out against the Malaria Box and compares these results with 55 assays that were previously published, in a format that allows a meta-analysis of the combined dataset. The combined biochemical and cellular assays presented here suggest mechanisms of action for 135 (34%) of the compounds active in killing multiple life-cycle stages of the malaria parasite, including asexual blood, liver, gametocyte, gametes and insect ookinete stages. In addition, many compounds demonstrated activity against other pathogens, showing hits in assays with 16 protozoa, 7 helminths, 9 bacterial and mycobacterial species, the dengue fever mosquito vector, and the NCI60 human cancer cell line panel of 60 human tumor cell lines. Toxicological, pharmacokinetic and metabolic properties were collected on all the compounds, assisting in the selection of the most promising candidates for murine proof-of-concept experiments and medicinal chemistry programs. The data for all of these assays are presented and analyzed to show how outstanding leads for many indications can be selected. These results reveal the immense potential for translating the dispersed expertise in biological assays involving human pathogens into drug discovery starting points, by providing open access to new families of molecules, and emphasize how a small additional investment made to help acquire and distribute compounds, and sharing the data, can catalyze drug discovery for dozens of different indications. Another lesson is that when multiple screens from different groups are run on the same library, results can be integrated quickly to select the most valuable starting points for subsequent medicinal chemistry efforts.
C1 [Van Voorhis, Wesley C.; Crowther, Gregory J.; Hewitt, Stephen Nakazawa; Ojo, Kayode K.] Univ Washington, Dept Med, CERID, Seattle, WA 98195 USA.
[Van Voorhis, Wesley C.; Crowther, Gregory J.; Hewitt, Stephen Nakazawa; Ojo, Kayode K.] Univ Washington, Dept Microbiol, CERID, Seattle, WA 98195 USA.
[Van Voorhis, Wesley C.; Crowther, Gregory J.; Hewitt, Stephen Nakazawa; Ojo, Kayode K.] Univ Washington, Dept Global Hlth, CERID, Seattle, WA 98195 USA.
[Adams, John H.; Jiang, Rays H. Y.; Kyle, Dennis E.; Pradhan, Anupam; Rice, Christopher A.; Udenze, Kenneth O.] Univ S Florida, Dept Global Hlth, Ctr Global Hlth & Infect Dis Res, Tampa, FL USA.
[Adelfio, Roberto; Cowan, Noemi; Ingram-Sieber, Katrin; Keiser, Jennifer; Panic, Gordana] Swiss Trop & Publ Hlth Inst, Med Parasitol & Infect Biol, Basel, Switzerland.
[Adelfio, Roberto; Cowan, Noemi; Ingram-Sieber, Katrin; Keiser, Jennifer; Panic, Gordana] Univ Basel, Basel, Switzerland.
[Ahyong, Vida; DeRisi, Joseph L.; Wu, Wesley] Univ Calif San Francisco, Dept Biochem & Biophys, Howard Hughes Med Inst, San Francisco, CA 94143 USA.
[Akabas, Myles H.] Albert Einstein Coll Med, Dept Physiol & Biophys, New York, NY USA.
[Akabas, Myles H.] Albert Einstein Coll Med, Dept Neurosci, New York, NY USA.
[Akabas, Myles H.] Albert Einstein Coll Med, Dept Med, New York, NY USA.
[Alano, Pietro; Camarda, Grazia; Silvestrini, Francesco] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, Rome, Italy.
[Alday, Aintzane; Alzualde, Ainhoa; Muriana, Arantza; Quevedo, Celia] BBD BioPhenix SL BIOBIDE, Donostia San Sebastian, Gipuzkoa, Spain.
[Resto, Yesmalie Aleman; Robledo, Jose A. Fernandez] Bigelow Lab Ocean Sci, East Boothbay, ME USA.
[Alsibaee, Aishah; Brennan, Marian; Chubb, Anthony] Royal Coll Surgeons Ireland, Mol & Cellular Therapeut, Dublin, Ireland.
[Andrews, Katherine T.; Avery, Vicky M.; Duffy, Sandra; Lucantoni, Leonardo; Nawaratna, Sujeevi; Ngoc Pham; Poulsen, Sally-Ann; Quinn, Ronald J.; Tonissen, Kathryn F.; Hoan Vu] Griffith Univ, Eskitis Inst Drug Discovery, Nathan, Qld, Australia.
[Andrews, Katherine T.; Nawaratna, Sujeevi] QIMR Berghofer Med Res Inst Herston, Brisbane, Qld, Australia.
[Avery, Simon V.; Narraidoo, Nathalie; Vallieres, Cindy] Univ Nottingham, Sch Life Sci, Nottingham, Notts, England.
[Ayong, Lawrence; Hansen, Michael A. E.; Lee, Sukjun; Moon, Seunghyun] Inst Pasteur Korea, Pangyo Techno Valley, Gyeonggi Provin, South Korea.
[Baker, Mark] Novartis Consumer Hlth, Clin Pharmacol, Nyon, Switzerland.
[Baker, Stephen; Phat Voong Vinh] Univ Oxford, Clin Res Unit, Wellcome Trust Major Overseas Programme, Hosp Trop Dis, Ho Chi Minh City, Vietnam.
[Baker, Stephen] Univ Oxford, Nuffield Dept Clin Med, Ctr Trop Med, Oxford, England.
[Baker, Stephen] London Sch Hyg & Trop Med, London, England.
[Ben Mamoun, Choukri; Kumar, Vidya P.] Yale Univ, Internal Med, New Haven, CT USA.
[Bhatia, Sangeeta; Gural, Nil; March, Sandra] MIT, Inst Med Engn & Sci, Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA.
[Bickle, Quentin; Mansour, Nuha R.] London Sch Hyg & Trop Med, Dept Immunol & Infect, London, England.
[Bounaadja, Lotfi; Florent, Isabelle] Sorbonne Univ, Museum Natl Hist, Paris, France.
[Bowling, Tana; Burton, Audrey; Mercer, Luke; Nare, Bakela] SCYNEXIS Inc, Durham, NC USA.
[Bosch, Juergen; Boucher, Lauren E.; Colon-Lopez, Daisy D.; Robinett, Natalie G.; Stamm, Serge Maximilian] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Biochem & Mol Biol, Baltimore, MD USA.
[Bosch, Juergen; Boucher, Lauren E.; Colon-Lopez, Daisy D.; Robinett, Natalie G.; Stamm, Serge Maximilian] Johns Hopkins Bloomberg Sch Publ Hlth, Johns Hopkins Malaria Res Inst, Baltimore, MD USA.
[Boyom, Fabrice F.; Fokou, Patrick V. T.; Mfopa, Alvine N.; Tchokouaha, Lauve R. Y.; Zollo, Paul H. A.] Univ Yaounde, Dept Biochem, Yaounde, Cameroon.
[Brea, Jose; Loza, Maria I.] Univ Santiago de Compostela, CIMUS Res Ctr, Santiago De Compostela, A Coruna, Spain.
[Caffrey, Conor R.; Suzuki, Brian M.] Univ Calif San Francisco, Dept Pathol, Ctr Discovery & Innovat Parasit Dis, San Francisco, CA 94140 USA.
[Carrasquilla, Manuela; Llinas, Manuel; Samra, Jasmeet] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA.
[Carrasquilla, Manuela; Llinas, Manuel; Samra, Jasmeet] Penn State Univ, Huck Ctr Malaria Res, University Pk, PA 16802 USA.
[Carter, Dee; Weatherby, Kate] Univ Sydney, Sch Life & Environm Sci, Darlington, NSW, Australia.
[Cassera, Maria Belen] Virginia Polytech Inst & State Univ, Dept Biochem, Blacksburg, VA 24061 USA.
[Cassera, Maria Belen] Virginia Polytech Inst & State Univ, Virginia Tech Ctr Drug Discovery, Blacksburg, VA 24061 USA.
[Cheng, Ken Chih-Chien; Guha, Rajarshi; Jadhav, Ajit] NIH, Natl Ctr Adv Translat Sci, Bldg 10, Bethesda, MD 20892 USA.
[Chindaudomsate, Worathad; Jensen, Laran T.] Mahidol Univ, Dept Biochem, Bangkok, Thailand.
[Colon, Beatrice L.] Univ S Florida, Morsani Coll Med, Dept Mol Med, Tampa, FL USA.
[Corbett, Yolanda; D'Alessandro, Sarah; Parapini, Silvia] Univ Milan, Dept Pharmacol & Biomol Sci, Milan, Italy.
[Le Dang, Na; Hughes, Tyler B.; Taramelli, Donatella] Washington Univ, Dept Pathol & Immunol, St Louis, MO USA.
[Delves, Michael; Ruecker, Andrea; Swamidass, S. Joshua] Imperial Coll London, Dept Life Sci, London, England.
[Du, Alan Y.; Meister, Stephan; Vigil, Edgar; Winzeler, Elizabeth A.; Wree, Melanie] Univ Calif San Diego, Sch Med, Dept Pediat, Div Pharmacol & Drug Discovery, La Jolla, CA 92093 USA.
[El-Sayed, Shimaa Abd El-Salam; Igarashi, Ikuo; Rizk, Mohamed Abdo; Yokoyama, Naoaki] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido, Japan.
[El-Sayed, Shimaa Abd El-Salam] Mansoura Univ, Dept Biochem & Chem Nutr, Mansoura, Egypt.
[Ferdig, Michael T.] Univ Notre Dame, Dept Biol Sci, Eck Inst Global Hlth, Notre Dame, IN 46556 USA.
[Fidock, David A.] Columbia Univ, Dept Med, Med Ctr, Dept Microbiol & Immunol, New York, NY USA.
[Fidock, David A.] Columbia Univ, Dept Med, Med Ctr, Div Infect Dis, New York, NY USA.
[Galstian, Ani; Golub, Todd; Thomas, David] Broad Inst, Cambridge, MA USA.
[Javier Gamo, Francisco; Jose Lafuente, Maria; Prats, Sarah] GlaxoSmithKline R&D, Biochem & Parasitol Dept, Malaria DPU, DDW, Madrid, Spain.
[Gokool, Suzanne; Townson, Simon] Northwick Pk Inst Med Res, Trop Parasit Dis Unit, Harrow, Middx, England.
[Gold, Ben; Ketas, Thomas; Little, David; Moore, John P.; Nathan, Carl] Weill Cornell Med Coll, Dept Microbiol & Immunol, New York, NY USA.
[Goldgof, Gregory M.] Univ Calif San Diego, Med Scientist Training Program, San Diego, CA 92103 USA.
[Guiguemde, W. Armand; Guy, R. Kiplin] St Jude Childrens Res Hosp, Dept Chem Biol & Therapeut, 332 N Lauderdale St, Memphis, TN 38105 USA.
[Hanson, Kirsten K.] Univ Texas San Antonio, Dept Biol, San Antonio, TX USA.
[Hanson, Kirsten K.] Univ Texas San Antonio, South Texas Ctr Emerging Infect Dis, San Antonio, TX USA.
[Hanson, Kirsten K.; Itoe, Maurice A.; Vera, Iset Medina] Inst Med Mol, Lisbon, Portugal.
[Hemphill, Andrew; Mueller, Joachim] Univ Bern, Inst Parasitol, Bern, Switzerland.
[van Huijsduijnen, Rob Hooft; Lee, Nathan; Abla, Nada; Blasco, Benjamin; Burrows, Jeremy; Laleu, Benoit; Leroy, Didier; Spangenberg, Thomas; Wells, Timothy; Willis, Paul A.] Med Malaria Venture, Geneva, Switzerland.
[Horii, Takaaki; Watanabe, Nao-aki] Eisai & Co Ltd, Hhc Data Creat Ctr, Global Hlth Res Sect, Tsukuba, Ibaraki, Japan.
[Horrocks, Paul; Ullah, Imran] Keele Univ, Inst Sci & Technol Med, Keele, Staffs, England.
[Huston, Christopher] Univ Vermont, Coll Med, Dept Med, Burlington, VT 05405 USA.
[Jensen, Amornrat Naranuntarat] Mahidol Univ, Fac Sci, Dept Pathobiol, Bangkok, Thailand.
[Kaiser, Annette] Inst Pharmacogenet, Med Res Ctr, Essen, Germany.
[Kicka, Sebastien; Soldati, Thierry; Trofimov, Valentin] Univ Geneva, Dept Biochem, Geneva, Switzerland.
[Kim, Sunyoung; Liu, Liqiong; Wojcik, Edward] LSU Hlth Sci Ctr, Dept Biochem & Mol Biol, New Orleans, LA USA.
[Kirk, Kiaran; Lehane, Adele M.] Australian Natl Univ, Res Sch Biol, Canberra, ACT, Australia.
[Landfear, Scott; Li, Fengwu; Ortiz, Diana] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97201 USA.
[Lubar, Aristea; Patra, Kailash; Vinetz, Joseph M.] Univ Calif San Diego, Dept Med, San Diego, CA 92103 USA.
[Lucet, Isabelle] Monash Univ, Dept Biochem & Mol Biol, Clayton, Vic, Australia.
[Maes, Louis] Univ Antwerp, Dept Biomed Sci, Antwerp, Belgium.
[Mancama, Dalu; Theron, Anjo] CSIR, Biosci Unit, Pretoria, South Africa.
[McGowan, Sheena] Monash Univ, Dept Microbiol, Clayton, Vic, Australia.
[Mestres, Jordi; Spitzmueller, Andreas] IMIM Hosp del Mar, Inst Med Res, Chemotargets SL, Barcelona, Catalonia, Spain.
[Mestres, Jordi; Spitzmueller, Andreas] IMIM Hosp del Mar, Inst Med Res, Res Grp Syst Pharmacol, Res Program Biomed Informat GRIB, Barcelona, Catalonia, Spain.
[Mestres, Jordi; Spitzmueller, Andreas] Univ Pompeu Fabra, Barcelona, Catalonia, Spain.
[Misra, Raj N.] NCI, Div Canc Therapeut & Diag, Drug Synth & Chem Branch, NIH, Bethesda, MD 20892 USA.
[Rodrigues da Costa, Francielly Morais] Univ Fed Minas Gerais, Grad Program Bioinformat, Belo Horizonte, MG, Brazil.
[Papadatos, George] EBI, EMBL, ChEMBL Grp, Hinxton, Cambs, England.
[Plouffe, David M.] Novartis Res Fdn, Genom Inst, San Diego, CA USA.
[Rizk, Mohamed Abdo] Mansoura Univ, Dept Internal Med & Infect Dis, Fac Vet Med, Mansoura, Egypt.
[St Onge, Robert; Schlecht, Ulrich] Stanford Univ, Dept Biochem, Palo Alto, CA 94304 USA.
[St Onge, Robert; Schlecht, Ulrich] Stanford Univ, Stanford Genome Technol Ctr, Palo Alto, CA 94304 USA.
[Ferreira, Rafaela Salgado; Villela, Filipe Silva; Suresh, Sundari] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, Brazil.
[Robinett, Natalie G.; Schmitt, Marjorie] IRJBD, Lab Chim Mol, COB, CNRS,UMR 7509, Mulhouse, France.
[Sinden, Robert] Univ Oxford, Jenner Inst, Oxford, England.
[Smith, Dennis A.] Univ Cape Town, Dept Chem, Cape Town, South Africa.
[Sullivan, David J.; Tripathi, Abhai K.] Johns Hopkins Sch Publ Hlth, H Feinstone Dept Mol Microbiol & Immunol, Baltimore, MD USA.
[Sullivan, William; White, Pamela M.] Univ Calif Santa Cruz, Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA.
[Suzuki, Yo] J Craig Venter Inst, Dept Synthet Biol & Bioenergy, La Jolla, CA USA.
[Tonissen, Kathryn F.] Griffith Univ, Sch Nat Sci, Nathan, Qld, Australia.
[Wilks, Andrew F.] Walter & Eliza Hall Inst Med Res, Hudson Inst Med Res, Parkville, Vic, Australia.
[Wilks, Andrew F.] SYNth Res, Parkville, Vic, Australia.
[Carrasquilla, Manuela] Wellcome Trust Sanger Inst, Wellcome Genome Campus, Hinxton, Cambs, England.
[Spangenberg, Thomas] Merck, Global Hlth, Coinsins, Switzerland.
RP Van Voorhis, WC (reprint author), Univ Washington, Dept Med, CERID, Seattle, WA 98195 USA.; Van Voorhis, WC (reprint author), Univ Washington, Dept Microbiol, CERID, Seattle, WA 98195 USA.; Van Voorhis, WC (reprint author), Univ Washington, Dept Global Hlth, CERID, Seattle, WA 98195 USA.
EM wesley@uw.edu
RI Quinn, Ronald/A-7931-2008; Gasull, Martina/A-6630-2013; Kirk,
Kiaran/C-8299-2009; Maes, Louis/A-2384-2017; Adams, John/G-1800-2015;
Poulsen, Sally-Ann/C-4500-2008; duffy, sandra/B-8479-2017; Tonissen,
Kathryn/E-9424-2010; Andrews, Katherine/F-9586-2011; Mestres,
Jordi/B-3673-2009;
OI McGowan, Sheena/0000-0001-6863-1106; Avery, Simon/0000-0002-2102-2255;
Rice, Christopher/0000-0003-0672-9604; SOLDATI,
Thierry/0000-0002-2056-7931; Quinn, Ronald/0000-0002-4022-2623; Kirk,
Kiaran/0000-0002-5613-2622; Maes, Louis/0000-0002-2324-9509; Adams,
John/0000-0003-3707-7979; Poulsen, Sally-Ann/0000-0003-4494-3687; duffy,
sandra/0000-0002-2814-1193; Tonissen, Kathryn/0000-0002-1018-2798;
Andrews, Katherine/0000-0002-1591-8979; Mestres,
Jordi/0000-0002-5202-4501; Du, Alan/0000-0003-3223-9705; Crowther,
Gregory/0000-0003-0530-9130; alano, pietro/0000-0003-0092-9840
FU UK DFID; Bill and Melinda Gates Foundation Grand Challenges
Explorations; Medicines for Malaria Venture MMV Challenge Grant [MMV
12/0048, MMV 12/0076]; Australian Research Council [FT10100185,
FT0991213, LP120200557]; Bill & Melinda Gates Foundation [OPP1040394,
OPP1040399, OPP1086189, OPP1069393, OPP1119049, OPP1024029]; Bloomberg
Family Foundation (JBr); JHMRI; US NIH [T32GM080189, R01GM104486,
R01AI117017]; National Science Foundation Graduate Research Fellowship
Program [DGE-1232825]; South African Medical Research Council [V6YBT51];
Council for Scientific and Industrial Research [V1YTB95]; French ANR
program Mammamia [ANR-12-BS07-0020-01]
FX Thanks to the UK DFID and the Bill and Melinda Gates Foundation Grand
Challenges Explorations for providing funding for testing of the Malaria
Box and funding the support of individual groups including: Medicines
for Malaria Venture MMV Challenge Grant, Grant Numbers MMV 12/0048 and
MMV 12/0076 (to JHA), the Australian Research Council (FT10100185 to
SAP; FT0991213 to KTA and LP120200557 awarded to VMA), Bill & Melinda
Gates Foundation Grant OPP1040394 to PA, OPP1040399 to DAF and VMA and
OPP1086189 to KKH, OPP1069393 and OPP1119049 to ML, OPP1024029 to CN,
the Bloomberg Family Foundation (JBr), JHMRI for a predoctoral
fellowship, the US NIH for the CBI training grant T32GM080189 (to LEB),
R01GM104486 (to PAW & WS), R01AI117017 (to JHA) the National Science
Foundation Graduate Research Fellowship Program Grant No. DGE-1232825
(DDCL), the South African Medical Research Council Strategic Health
Innovation Partnerships (grant V6YBT51 to DM) and the Council for
Scientific and Industrial Research (grant V1YTB95, to DM), and the
French ANR program Mammamia (ANR-12-BS07-0020-01). The funders had no
role in study design, data collection and analysis, decision to publish,
or preparation of the manuscript.
NR 75
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U1 12
U2 15
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-7366
EI 1553-7374
J9 PLOS PATHOG
JI PLoS Pathog.
PD JUL
PY 2016
VL 12
IS 7
AR e1005763
DI 10.1371/journal.ppat.1005763
PG 23
WC Microbiology; Parasitology; Virology
SC Microbiology; Parasitology; Virology
GA DW0XN
UT WOS:000383366400043
PM 27467575
ER
PT J
AU Hsu, PF
Cheng, HM
Wu, CH
Sung, SH
Chuang, SY
Lakatta, EG
Yin, FCP
Chou, P
Chen, CH
AF Hsu, Pai-Feng
Cheng, Hao-Min
Wu, Cheng-Hsueh
Sung, Shih-Hsien
Chuang, Shao-Yuan
Lakatta, Edward G.
Yin, Frank C. P.
Chou, Pesus
Chen, Chen-Huan
TI High Short-Term Blood Pressure Variability Predicts Long-Term
Cardiovascular Mortality in Untreated Hypertensives But Not in
Normotensives
SO AMERICAN JOURNAL OF HYPERTENSION
LA English
DT Article
DE ambulatory blood pressure; blood pressure variability; cardiovascular
mortality; hypertension
ID LEFT-VENTRICULAR MASS; TARGET-ORGAN DAMAGE; E-LORO-ASSOCIAZIONI;
PROGNOSTIC VALUE; GENERAL-POPULATION; HOME; MANAGEMENT; ARTERIAL;
TAIWAN; DEATH
AB BACKGROUND
The prognostic value of the short-term blood pressure variability (BPV) from the 24-hour ambulatory blood pressure monitoring (ABPM) remains controversial. The present study aimed to investigate the longterm prognostic value of a high BPV in normotensive and hypertensive subjects from a community-based population.
METHODS
A cohort of 624 normotensive and 633 untreated hypertensive Taiwanese participants (overall 669 men, aged 30-79 years) with baseline ABPM and 20-year all-cause and cardiovascular mortality data was drawn from a community-based survey. BPV was assessed by the read-to-read average real variability of the 24-hour diastolic and systolic blood pressure (SBP) (ARVd and ARVs, respectively).
RESULTS
In Cox proportional hazards analysis, ARVd predicted cardiovascular mortality independently of office SBP (hazard ratios (HRs) and 95% confidence intervals (CIs) per 1 SD: 1.31 (1.10-1.55), respectively, bivariate analysis), 24-hour SBP (HR: 1.19, 95% CI: 1.00-1.43), and conventional risk factors (age, sex, smoking, total cholesterol, high-density lipoprotein cholesterol, and fasting blood glucose, HR: 1.40, 95% CI: 1.18-1.67). In subjects with hypertension, a high vs. low ARVd (median: 8.8 mm Hg) significantly predicted cardiovascular mortality (HR: 2.11, 95% CI: 1.23-3.62 and HR: 2.04, 95% CI: 1.19-3.51, respectively), when the conventional risk factors plus office SBP or 24-hour SBP were accounted for, respectively. Similar but less significant results were obtained with ARVs. A high ARVd or ARVs did not significantly predict cardiovascular mortality in the normotensive subjects.
CONCLUSIONS
A high short-term BPV is significantly predictive of long-term cardiovascular mortality in untreated hypertensive but not normotensive community-based subjects, independently of office or 24-hour SBP.
C1 [Hsu, Pai-Feng; Cheng, Hao-Min; Wu, Cheng-Hsueh; Sung, Shih-Hsien; Chen, Chen-Huan] Taipei Vet Gen Hosp, Dept Med, Taipei, Taiwan.
[Hsu, Pai-Feng; Cheng, Hao-Min; Sung, Shih-Hsien; Chou, Pesus; Chen, Chen-Huan] Natl Yang Ming Univ, Inst Publ Hlth, Taipei, Taiwan.
[Hsu, Pai-Feng; Wu, Cheng-Hsueh; Sung, Shih-Hsien; Chen, Chen-Huan] Natl Yang Ming Univ, Fac Med, Taipei, Taiwan.
[Cheng, Hao-Min; Chen, Chen-Huan] Taipei Vet Gen Hosp, Dept Med Educ, Taipei, Taiwan.
[Chuang, Shao-Yuan] Natl Hlth Res Inst, Miaoli, Taiwan.
[Lakatta, Edward G.] NIA, Cardiovasc Sci Lab, Intramural Res Program Baltimore, Baltimore, MD 21224 USA.
[Yin, Frank C. P.] Washington Univ, Dept Biomed Engn, St Louis, MO 63105 USA.
RP Chen, CH (reprint author), Taipei Vet Gen Hosp, Dept Med, Taipei, Taiwan.; Chen, CH (reprint author), Natl Yang Ming Univ, Inst Publ Hlth, Taipei, Taiwan.; Chen, CH (reprint author), Natl Yang Ming Univ, Fac Med, Taipei, Taiwan.; Chen, CH (reprint author), Taipei Vet Gen Hosp, Dept Med Educ, Taipei, Taiwan.
EM chench@vghtpe.gov.tw
FU National Science Council [NSC 99-2314-B-010-034-MY3, MOST
103-2314-B-010-050-MY2]; Taipei Veterans General Hospital [V104C-140];
Ministry of Health and Welfare [NO1-AG-1-2118,
MOHW104-TDU-B-211-113-003]; Intramural Research Program of the National
Institute on Aging, National Institutes of Health
FX This work was supported in part by grants from the National Science
Council (NSC 99-2314-B-010-034-MY3 and MOST 103-2314-B-010-050-MY2), an
intramural grant from the Taipei Veterans General Hospital (grant
V104C-140), Research and Development contract NO1-AG-1-2118, grants from
the Ministry of Health and Welfare (MOHW104-TDU-B-211-113-003), and the
Intramural Research Program of the National Institute on Aging, National
Institutes of Health.
NR 24
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Z9 2
U1 1
U2 1
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0895-7061
EI 1941-7225
J9 AM J HYPERTENS
JI Am. J. Hypertens.
PD JUL
PY 2016
VL 29
IS 7
BP 806
EP 813
DI 10.1093/ajh/hpw002
PG 8
WC Peripheral Vascular Disease
SC Cardiovascular System & Cardiology
GA DV8HL
UT WOS:000383178000004
PM 26837643
ER
PT J
AU Horbal, SR
Seffens, W
Davis, AR
Silvestrov, N
Gibbons, GH
Quarells, RC
Bidulescu, A
AF Horbal, Steven R.
Seffens, William
Davis, Adam R.
Silvestrov, Natalia
Gibbons, Gary H.
Quarells, Rakale C.
Bidulescu, Aurelian
TI Associations of Apelin, Visfatin, and Urinary 8-Isoprostane With Severe
Hypertension in African Americans: The MH-GRID Study
SO AMERICAN JOURNAL OF HYPERTENSION
LA English
DT Article
DE adipokines; African Americans; apelin; blood pressure; hypertension;
severe hypertension; urinary 8-isoprostane; visfatin
ID ADIPOSE-TISSUE; ENDOTHELIAL DYSFUNCTION; RESISTANT HYPERTENSION;
INFLAMMATION; OBESITY; ADIPOKINES
AB BACKGROUND
Apelin is an adipokine directly associated with adiposity, insulin resistance, and decreased blood pressure. Urinary 8-isoprostane is a marker of chronic oxidative endothelial stress. Visfatin, an adipokine that acts by binding and activating the insulin receptor, has been associated with hypertension. As severe hypertension (SH) is highly prevalent among African Americans (AA), we aimed to assess the association of these biomarkers with SH status.
METHODS
A sample of 250 AA participants (134 normotensive controls and 116 with SH (including 98 treatment controlled, SCH: severe controlled hypertension, and 18 treatment resistant, SRH: severe resistant hypertension)) from the Minority Health Genomics and Translational Research Bio-Repository Database (MH-GRID) in metro Atlanta had blood analyzed for apelin and visfatin and urine for 8-isoprostane. T-tests, sex-specific age-adjusted correlation coefficients, and multivariable logistic regression models were used to assess the association of biomarkers with hypertensive status.
RESULTS
Levels of apelin and 8-isoprostane were not statistically different between controls and SCH or SRH. Statistically significant differences were present in levels of visfatin between controls (1.03 +/- 0.84 pg/ml), SCH (1.34 +/- 1.14 pg/ml), and SRH (1.59 +/- 0.85 pg/ml). After multivariable adjustment, categorization in the middle 2 quartiles of urinary 8-isoprostane were associated with SH. In similar models, categorization into the highest quartile of visfatin was associated with SH (odds ratio = 2.80; 95% confidence interval: 1.02-7.02). A continuous association of visfatin with SH was present.
CONCLUSION In our community sample of AA, there were increased odds of SH with increased levels of urinary 8-isoprostane and visfatin, but not with apelin.
C1 [Horbal, Steven R.; Bidulescu, Aurelian] Indiana Univ, Sch Publ Hlth, Dept Epidemiol & Biostat, Bloomington, IN 47405 USA.
[Seffens, William; Silvestrov, Natalia; Quarells, Rakale C.] Morehouse Sch Med, Dept Community Hlth & Prevent Med, Atlanta, GA 30310 USA.
[Davis, Adam R.] NHGRI, Bethesda, MD 20892 USA.
[Gibbons, Gary H.] NHLBI, NIH, Bethesda, MD 20892 USA.
RP Bidulescu, A (reprint author), Indiana Univ, Sch Publ Hlth, Dept Epidemiol & Biostat, Bloomington, IN 47405 USA.
EM abidules@indiana.edu
FU NIH/National Institute on Minority Health and Health Disparities
[1RC4MD005964]
FX We would like to acknowledge the Minority Health-GRID Network (Rakale C.
Quarells, Gary H. Gibbons, Donna K. Arnett, Robert L. Davis, Suzanne M.
Leal, Deborah A. Nickerson, James Perkins, Charles N. Rotimi, Joel H.
Saltz, Herman A. Taylor, and James G. Wilson). This work was supported,
in part, by a grant from the NIH/National Institute on Minority Health
and Health Disparities (grant #1RC4MD005964).
NR 22
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Z9 0
U1 2
U2 2
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0895-7061
EI 1941-7225
J9 AM J HYPERTENS
JI Am. J. Hypertens.
PD JUL
PY 2016
VL 29
IS 7
BP 814
EP 820
DI 10.1093/ajh/hpw007
PG 7
WC Peripheral Vascular Disease
SC Cardiovascular System & Cardiology
GA DV8HL
UT WOS:000383178000005
PM 26869250
ER
PT J
AU Trasande, L
Zoeller, RT
Hass, U
Kortenkamp, A
Grandjean, P
Myers, JP
DiGangi, J
Hunt, PM
Rudel, R
Sathyanarayana, S
Bellanger, M
Hauser, R
Legler, J
Skakkebaek, NE
Heindel, JJ
AF Trasande, L.
Zoeller, R. T.
Hass, U.
Kortenkamp, A.
Grandjean, P.
Myers, J. P.
DiGangi, J.
Hunt, P. M.
Rudel, R.
Sathyanarayana, S.
Bellanger, M.
Hauser, R.
Legler, J.
Skakkebaek, N. E.
Heindel, J. J.
TI Burden of disease and costs of exposure to endocrine disrupting
chemicals in the European Union: an updated analysis
SO ANDROLOGY
LA English
DT Article
DE disease burden; economic costs; endocrine disrupting chemicals
ID REPRODUCTIVE DISORDERS; HEALTH; RECOMMENDATIONS; STRENGTH; QUALITY;
GRADE
AB A previous report documented that endocrine disrupting chemicals contribute substantially to certain forms of disease and disability. In the present analysis, our main objective was to update a range of health and economic costs that can be reasonably attributed to endocrine disrupting chemical exposures in the European Union, leveraging new burden and disease cost estimates of female reproductive conditions from accompanying report. Expert panels evaluated the epidemiologic evidence, using adapted criteria from the WHO Grading of Recommendations Assessment, Development and Evaluation Working Group, and evaluated laboratory and animal evidence of endocrine disruption using definitions recently promulgated by the Danish Environmental Protection Agency. The Delphi method was used to make decisions on the strength of the data. Expert panels consensus was achieved for probable (>20%) endocrine disrupting chemical causation for IQ loss and associated intellectual disability; autism; attention deficit hyperactivity disorder; endometriosis; fibroids; childhood obesity; adult obesity; adult diabetes; cryptorchidism; male infertility, and mortality associated with reduced testosterone. Accounting for probability of causation, and using the midpoint of each range for probability of causation, Monte Carlo simulations produced a median annual cost of (sic)163 billion (1.28% of EU Gross Domestic Product) across 1000 simulations. We conclude that endocrine disrupting chemical exposures in the EU are likely to contribute substantially to disease and dysfunction across the life course with costs in the hundreds of billions of Euros per year. These estimates represent only those endocrine disrupting chemicals with the highest probability of causation; a broader analysis would have produced greater estimates of burden of disease and costs.
C1 [Trasande, L.] NYU, Sch Med, New York, NY USA.
[Trasande, L.] NYU, Wagner Sch Publ Serv, New York, NY USA.
[Trasande, L.] NYU, Dept Nutr Food & Publ Hlth, Steinhardt Sch Culture Educ & Human Dev, New York, NY USA.
[Trasande, L.] NYU, Global Inst Publ Hlth, New York, NY USA.
[Zoeller, R. T.] Univ Massachusetts, Amherst, MA 01003 USA.
[Hass, U.] Tech Univ Denmark, Natl Food Inst, Soborg, Denmark.
[Kortenkamp, A.] Brunel Univ, Inst Environm Hlth & Soc, Uxbridge, Middx, England.
[Grandjean, P.; Hauser, R.] Harvard TH Chan Sch Publ Hlth, Dept Environm Hlth, Boston, MA USA.
[Grandjean, P.] Univ Southern Denmark, Odense, Denmark.
[Myers, J. P.] Environm Hlth Sci, Charlottesville, VA USA.
[DiGangi, J.] IPEN, Gothenburg, Sweden.
[Hunt, P. M.] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA.
[Rudel, R.] Silent Spring Inst, Newton, MA USA.
[Sathyanarayana, S.] Seattle Childrens Res Inst, Ctr Child Hlth Behav & Dev, Seattle, WA USA.
[Bellanger, M.] EHESP Sch Publ Hlth, Paris, France.
[Legler, J.] Vrije Univ Amsterdam, Inst Environm Studies, Dept Biol & Chem, Amsterdam, Netherlands.
[Skakkebaek, N. E.] EDMaRC, Rigshosp, Dept Growth & Reprod, Copenhagen, Denmark.
[Skakkebaek, N. E.] Univ Copenhagen, Copenhagen, Denmark.
[Heindel, J. J.] NIEHS, Div Extramural Res & Training, POB 12233, Res Triangle Pk, NC 27709 USA.
RP Trasande, L (reprint author), NYU, Sch Med, Dept Pediat, 403 East 34th St,Room 115, New York, NY 10016 USA.
EM leonardo.trasande@nyumc.org
OI Legler, Juliette/0000-0001-6321-1567
FU Endocrine Society; John Merck Fund; Broad Reach Foundation; Oak
Foundation; NIEHS Division of Extramural Research and Training
FX Research reported in this publication was supported by the Endocrine
Society, the John Merck Fund, the Broad Reach Foundation, and the Oak
Foundation. The funders and supporters had no role in the writing of the
manuscript or the decision to submit it for publication. The content is
solely the responsibility of the authors and does not necessarily
represent the official views of the NIEHS, the National Institutes of
Health or the US government. We thank Charles Persoz, Robert Barouki and
Marion Le Gal of the French National Alliance for Life Sciences and
Health, and Barbara Demeneix, Lindsey Marshall, Bilal Mughal, and Bolaji
Seffou of the UMR 7221 Paris for providing technical and logistical
support throughout the project. We also wish to thank Roberto
Bertollini, Annette Pruss-Ustun, and David Tordrup of the World Health
Organization for their consultation and support in developing the
guidelines for evaluating epidemiologic evidence. The contribution of
JJH was supported by the NIEHS Division of Extramural Research and
Training.
NR 19
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U1 9
U2 9
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-2919
EI 2047-2927
J9 ANDROLOGY-US
JI Andrology
PD JUL
PY 2016
VL 4
IS 4
SI SI
BP 565
EP 572
DI 10.1111/andr.12178
PG 8
WC Andrology
SC Endocrinology & Metabolism
GA DV9VT
UT WOS:000383291000004
PM 27003928
ER
PT J
AU Louis, GMB
Barr, DB
Kannan, K
Chen, Z
Kim, S
Sundaram, R
AF Louis, G. M. Buck
Barr, D. B.
Kannan, K.
Chen, Z.
Kim, S.
Sundaram, R.
TI Paternal exposures to environmental chemicals and time-to-pregnancy:
overview of results from the LIFE study
SO ANDROLOGY
LA English
DT Article
DE endocrine disrupters; epidemiology; fecundity; men; time to pregnancy
ID ENDOCRINE-DISRUPTING CHEMICALS; URINARY BISPHENOL-A; TANDEM
MASS-SPECTROMETRY; TESTICULAR CANCER; HUMAN-SERUM; SCIENTIFIC STATEMENT;
REGIONAL DIFFERENCES; PROSPECTIVE COHORT; REGRESSION-MODELS; COUPLE
FECUNDITY
AB Published findings from the Longitudinal Investigation of Fertility and the Environment (LIFE) Study regarding the relation between environmental chemicals and couple fecundity, as measured by time-to-pregnancy (TTP), are reviewed with a particular focus on role of the male partner. The LIFE Study recruited 501 couples from 16 counties in two U.S. states upon discontinuing contraception for purposes of becoming pregnant. Upon enrollment, couples provided a blood and urine sample for the quantification of persistent and non-persistent environmental chemicals, respectively, and then completed daily journals until pregnant or up to one year of trying. Female partners used fertility monitors to aid the timing of intercourse relative to ovulation, and digital home pregnancy test kits on the day of expected menses. Chemical classes included: metals, persistent organic pollutants, environmental phenols, and phthalates that were quantified using inductively coupled plasma mass spectrometry or isotope dilution high-resolution or tandem mass spectrometry. Time-to-pregnancy (TTP) was defined as the number of prospectively observed menstrual cycles required for pregnancy. Fecundability odds ratios (FORs) and 95% confidence intervals (CIs) were estimated for each chemical and partner after adjusting for potential confounders and accounting for right censoring and time off contraception. FORs < 1 are suggestive of diminished fecundity or a longer TTP. Significant reductions (ranging from 17-31%) in couple fecundity were observed for male partners' concentration of lead (0.83; 0.70, 0.98), 2,2',4,4'-tetrahydroxybenzophenone (0.69; 0.49, 0.97), monobenzyl (0.80; 0.67, 0.97), and monomethyl (0.81; 0.70, 0.94) phthalates after adjusting for the female partners' concentrations. Seven PCB congeners quantified in men's serum were associated with a 17-29% reduction in couple fecundity. Our findings underscore the importance of a couple-based exposure design, inclusive of the male partner, when assessing couple-dependent outcomes such as TTP to avoid misinterpretation of results based only upon the female partner.
C1 [Louis, G. M. Buck] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Div Intramural Populat Hlth Res, NIH, 6100 Execut Blvd,Room 7B05, Rockville, MD 20852 USA.
[Barr, D. B.] Emory Univ, Dept Environm Hlth, Rollins Sch Publ Hlth, Atlanta, GA 30322 USA.
[Kannan, K.] New York State Dept Hlth, Wadsworth Ctr, Albany, NY USA.
[Chen, Z.; Kim, S.; Sundaram, R.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Biostat & Bioinformat Branch, Div Intramural Populat Hlth Res, NIH, Rockville, MD USA.
RP Louis, GMB (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Div Intramural Populat Hlth Res, NIH, 6100 Execut Blvd,Room 7B05, Rockville, MD 20852 USA.
EM louisg@mail.nih.gov
OI Sundaram, Rajeshwari/0000-0002-6918-5002; Buck Louis,
Germaine/0000-0002-1774-4490
FU Eunice Kennedy Shriver National Institute of Child Health and Human
Development [N01-HD-3-3355, N01-HD-3-3356, NOH-HD-3-3358, HHSN27500001,
HHSN2750002]; NICHD
FX This work was supported by the Intramural Research Program of the Eunice
Kennedy Shriver National Institute of Child Health and Human Development
(contracts #N01-HD-3-3355; N01-HD-3-3356; NOH-HD-3-3358; HHSN27500001;
HHSN2750002). Members of the LIFE Study acknowledge the laboratory staff
members at the Division of Laboratory Sciences, Centers for Disease
Control and Prevention who performed the analytic chemistry work for the
persistent chemicals under a Memo of Understanding with the NICHD, and
the laboratory staff members at the Wadsworth Center for the analysis of
non-persistent chemicals under contracts with the NICHD. This work was
presented on April 27, 2015 at the 8th Copenhagen Workshop on Endocrine
Disrupters.
NR 68
TC 0
Z9 0
U1 7
U2 7
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 2047-2919
EI 2047-2927
J9 ANDROLOGY-US
JI Andrology
PD JUL
PY 2016
VL 4
IS 4
SI SI
BP 639
EP 647
DI 10.1111/andr.12171
PG 9
WC Andrology
SC Endocrinology & Metabolism
GA DV9VT
UT WOS:000383291000012
ER
PT J
AU Mills, JL
Dimopoulos, A
Bailey, RL
AF Mills, James L.
Dimopoulos, Aggeliki
Bailey, Regan L.
TI What Is Standing in the Way of Complete Prevention of Folate Preventable
Neural Tube Defects?
SO BIRTH DEFECTS RESEARCH PART A-CLINICAL AND MOLECULAR TERATOLOGY
LA English
DT Editorial Material
ID FOLIC-ACID FORTIFICATION; POPULATION; TRENDS; SUPPLEMENTATION;
VITAMIN-B-12; INDIVIDUALS; EUROPE
C1 [Mills, James L.; Dimopoulos, Aggeliki] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Epidemiol Branch, Div Intramural Populat Hlth Res, NIH, Bethesda, MD 20892 USA.
[Bailey, Regan L.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA.
RP Mills, JL (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Epidemiol Branch, Div Intramural Populat Hlth Res, NIH, Bethesda, MD 20892 USA.
FU Intramural NIH HHS [Z99 HD999999]
NR 15
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PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1542-0752
EI 1542-0760
J9 BIRTH DEFECTS RES A
JI Birth Defects Res. Part A-Clin. Mol. Teratol.
PD JUL
PY 2016
VL 106
IS 7
BP 517
EP 519
DI 10.1002/bdra.23518
PG 3
WC Developmental Biology; Toxicology
SC Developmental Biology; Toxicology
GA DV3RH
UT WOS:000382840300002
PM 27418028
ER
PT J
AU Kannan, L
Ramos, M
Re, A
El-Hachem, N
Safikhani, Z
Gendoo, DMA
Davis, S
Gomez-Cabrero, D
Castelo, R
Hansen, KD
Carey, VJ
Morgan, M
Culhane, AC
Haibe-Kains, B
Waldron, L
AF Kannan, Lavanya
Ramos, Marcel
Re, Angela
El-Hachem, Nehme
Safikhani, Zhaleh
Gendoo, Deena M. A.
Davis, Sean
Gomez-Cabrero, David
Castelo, Robert
Hansen, Kasper D.
Carey, Vincent J.
Morgan, Martin
Culhane, Aedin C.
Haibe-Kains, Benjamin
Waldron, Levi
TI Public data and open source tools for multi-assay genomic investigation
of disease
SO BRIEFINGS IN BIOINFORMATICS
LA English
DT Article
DE multiple assays (multi-assays); public data; bioconductor; integrative
genomics; cancer; pharmacogenomics; omics
ID BRITISH BIRTH COHORT; CANONICAL CORRELATION-ANALYSIS; GENE-EXPRESSION
SIGNATURES; CANCER-CELL LINES; DRUG-SENSITIVITY; WIDE ASSOCIATION; SMALL
MOLECULES; COPY NUMBER; TUMOR TYPES; DATA SETS
AB Molecular interrogation of a biological sample through DNA sequencing, RNA and microRNA profiling, proteomics and other assays, has the potential to provide a systems level approach to predicting treatment response and disease progression, and to developing precision therapies. Large publicly funded projects have generated extensive and freely available multi-assay data resources; however, bioinformatic and statistical methods for the analysis of such experiments are still nascent. We review multi-assay genomic data resources in the areas of clinical oncology, pharmacogenomics and other perturbation experiments, population genomics and regulatory genomics and other areas, and tools for data acquisition. Finally, we review bioinformatic tools that are explicitly geared toward integrative genomic data visualization and analysis. This review provides starting points for accessing publicly available data and tools to support development of needed integrative methods.
C1 [Kannan, Lavanya; Ramos, Marcel] CUNY Hunter Coll, Sch Publ Hlth, New York, NY 10021 USA.
[Re, Angela] Univ Trento, Ctr Integrat Biol, Trento, Italy.
[El-Hachem, Nehme] Univ Montreal, Montreal, PQ H3C 3J7, Canada.
[Safikhani, Zhaleh; Gendoo, Deena M. A.; Haibe-Kains, Benjamin] Princess Margaret Canc Ctr, Toronto, ON, Canada.
[Davis, Sean] NCI, NIH, Bethesda, MD 20892 USA.
[Gomez-Cabrero, David] Karolinska Inst, Unit Computat Med, S-10401 Stockholm, Sweden.
[Castelo, Robert] Univ Pompeu Fabra, Bioinformat & Biostat, Barcelona, Spain.
[Hansen, Kasper D.] Johns Hopkins Univ, Biostat, Baltimore, MD 21218 USA.
[Carey, Vincent J.] Harvard Med Sch, Med Biostat, Boston, MA USA.
[Morgan, Martin] Roswell Pk Canc Inst, Biostat & Bioinformat, Buffalo, NY 14263 USA.
[Culhane, Aedin C.] Dana Farber Canc Inst, Boston, MA 02115 USA.
[Waldron, Levi] CUNY Hunter Coll, Sch Publ Hlth, Biostat, New York, NY 10021 USA.
RP Waldron, L (reprint author), 2180 3rd Ave, New York, NY 10040 USA.
EM levi.waldron@hunter.cuny.edu
RI Castelo, Robert/A-4679-2010; Gasull, Martina/A-6630-2013;
OI Castelo, Robert/0000-0003-2229-4508; Haibe-Kains,
Benjamin/0000-0002-7684-0079; Waldron, Levi/0000-0003-2725-0694
FU National Cancer Institute [U24CA180996, 1U19 AI111224-01]; National
Institute on Minority Health and Health Disparities [MD007599]; NCI
Intramural Research Program of the National Institutes of Health;
Spanish MINECO [TIN2011-22826]; Gattuso Slaight Personalized Cancer
Medicine Fund at Princess Margaret Cancer Centre; Cancer Research
Society (Canada); Brain Canada-CIBC Brain Cancer Research Training
Award; University of Trento; Breast Cancer Research Program
[W81XWH-15-1-0013]
FX The authors' work was funded by the National Cancer Institute
[U24CA180996 to MM], the National Institute on Minority Health and
Health Disparities [MD007599 to LW] and the NCI Intramural Research
Program [SD] of the National Institutes of Health. Spanish MINECO grant
TIN2011-22826 to RC. BHK was supported by the Gattuso Slaight
Personalized Cancer Medicine Fund at Princess Margaret Cancer Centre. ZS
was supported by the Cancer Research Society (Canada). DMAG was
supported by the Brain Canada-CIBC Brain Cancer Research Training Award
AR was supported by the Biotechnology start-up project of the University
of Trento. AC was supported by National Cancer Institute 1U19
AI111224-01 and the Assistant Secretary of Defense Health Program,
through the Breast Cancer Research Program, Award No. W81XWH-15-1-0013.
NR 96
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PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 1467-5463
EI 1477-4054
J9 BRIEF BIOINFORM
JI Brief. Bioinform.
PD JUL
PY 2016
VL 17
IS 4
SI SI
BP 603
EP 615
DI 10.1093/bib/bbv080
PG 13
WC Biochemical Research Methods; Mathematical & Computational Biology
SC Biochemistry & Molecular Biology; Mathematical & Computational Biology
GA DV7XI
UT WOS:000383151200005
PM 26463000
ER
PT J
AU Hart, SN
Maxwell, KN
Thomas, T
Ravichandran, V
Wubberhorst, B
Klein, RJ
Schrader, K
Szabo, C
Weitzel, JN
Neuhausen, SL
Nathanson, K
Offit, K
Couch, FJ
Vijai, J
AF Hart, Steven N.
Maxwell, Kara N.
Thomas, Tinu
Ravichandran, Vignesh
Wubberhorst, Bradley
Klein, Robert J.
Schrader, Kasmintan
Szabo, Csilla
Weitzel, Jeffrey N.
Neuhausen, Susan L.
Nathanson, Katherine
Offit, Kenneth
Couch, Fergus J.
Vijai, Joseph
TI Collaborative science in the next-generation sequencing era: a viewpoint
on how to combine exome sequencing data across sites to identify novel
disease susceptibility genes
SO BRIEFINGS IN BIOINFORMATICS
LA English
DT Article
DE genomics; exome
ID GENOME-WIDE ASSOCIATION; BREAST-CANCER; MISSING HERITABILITY; READ
ALIGNMENT; VARIANTS; ANNOTATION; ALGORITHMS; MUTATIONS; SINGLE; TOOLS
AB The purpose of this article is to inform readers about technical challenges that we encountered when assembling exome sequencing data from the 'Simplifying Complex Exomes' (SIMPLEXO) consortium-whose mandate is the discovery of novel genes predisposing to breast and ovarian cancers. Our motivation is to share these obstacles-and our solutions to them-as a means of communicating important technical details that should be discussed early in projects involving massively parallel sequencing.
C1 [Hart, Steven N.] Mayo Clin, Biomed Informat, Rochester, MN USA.
[Maxwell, Kara N.] Univ Penn, Perelman Sch Med, Div Hematol Oncol, Philadelphia, PA 19104 USA.
[Thomas, Tinu; Ravichandran, Vignesh] Mem Sloan Kettering Canc Ctr, Clin Genet Res Lab, 1275 York Ave, New York, NY 10021 USA.
[Klein, Robert J.] Icahn Sch Med Mt Sinai, New York, NY 10029 USA.
[Schrader, Kasmintan] Univ British Columbia, Vancouver, BC V5Z 1M9, Canada.
[Schrader, Kasmintan] BC Canc Agcy, Dept Med Genet, Vancouver, BC, Canada.
[Schrader, Kasmintan] BC Canc Agcy, Hereditary Canc Program, Vancouver, BC, Canada.
[Szabo, Csilla] NHGRI, NIH, Bethesda, MD 20892 USA.
[Weitzel, Jeffrey N.] City Hope Comprehens Canc Ctr, Div Clin Canc Genet, Duarte, CA USA.
[Weitzel, Jeffrey N.] City Hope Comprehens Canc Ctr, Canc Screening & Prevent Program, Duarte, CA USA.
[Weitzel, Jeffrey N.] City Hope Natl Med Ctr, Oncol & Populat Sci, Duarte, CA USA.
[Nathanson, Katherine] Abramson Canc Ctr, Div Translat Med, Philadelphia, PA USA.
[Nathanson, Katherine] Abramson Canc Ctr, Philadelphia, PA USA.
[Offit, Kenneth] Mem Hosp, Clin Genet Serv, Belleville, IL USA.
[Offit, Kenneth] Mem Sloan Kettering Canc Ctr, Sloan Kettering Inst, Canc Biol & Genet Program, 1275 York Ave, New York, NY 10021 USA.
[Couch, Fergus J.] Mayo Clin, Dept Lab Med & Pathol, Med Res, Rochester, MN USA.
[Couch, Fergus J.] Mayo Clin, Dept Lab Med & Pathol, Div Expt Pathol, Rochester, MN USA.
[Vijai, Joseph] Mem Sloan Kettering Canc Ctr, Dept Med, 1275 York Ave, New York, NY 10021 USA.
[Vijai, Joseph] Mem Sloan Kettering Canc Ctr, Clin Genet Res Lab, 1275 York Ave, New York, NY 10021 USA.
RP Hart, SN (reprint author), Mayo Clin, Mayo Coll Med, Dept Hlth Sci Res, Bioinformat,CGSL, 200 First St SW, Rochester, MN 55905 USA.; Hart, SN (reprint author), Mayo Clin, Mayo Coll Med, Dept Hlth Sci Res, Biomed Informat,Div Biomed Stat & Informat, 200 First St SW, Rochester, MN 55905 USA.; Vijai, J (reprint author), Mem Sloan Kettering Canc Ctr, Dept Med, Clin Genet Serv, New York, NY 10065 USA.
EM Hart.steven@mayo.edu; josephv@mskcc.org
FU National Institutes of Health [R01CA176785]; Breast Cancer Research
Foundation [R21-CA139396]; Department of Defense [W81XWH-13-1-0338];
Niehaus Clinical Genetics Initiative; Miele family initiative; National
Cancer Institute [RC4CA153828]; Office of the Director, National
Institutes of Health; MSKCC Core grant [NIH P30CA008748]
FX This work was supported by the National Institutes of Health R01CA176785
and the Breast Cancer Research Foundation [F.J.C., K.N. and K.O],
R21-CA139396 [J.V]. K.N.M. was supported by the Department of Defense
(W81XWH-13-1-0338); views and opinions of, and endorsements by, the
authors do not reflect those of the US Army or the Department of
Defense. Other funding was provided by The Niehaus Clinical Genetics
Initiative and The Miele family initiative [K.O.]. City of Hope Clinical
Cancer Genetics Community Research Network and the Hereditary Cancer
Research Registry, supported in part by Award Number RC4CA153828 (PI: J.
Weitzel) from the National Cancer Institute and the Office of the
Director, National Institutes of Health. The content is solely the
responsibility of the authors and does not necessarily represent the
official views of the National Institutes of Health. The work was also
supported by the MSKCC Core grant NIH P30CA008748.
NR 29
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PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 1467-5463
EI 1477-4054
J9 BRIEF BIOINFORM
JI Brief. Bioinform.
PD JUL
PY 2016
VL 17
IS 4
SI SI
BP 672
EP 677
DI 10.1093/bib/bbv075
PG 6
WC Biochemical Research Methods; Mathematical & Computational Biology
SC Biochemistry & Molecular Biology; Mathematical & Computational Biology
GA DV7XI
UT WOS:000383151200010
PM 26358132
ER
PT J
AU Wolf, C
Mohr, H
Diekhof, EK
Vieker, H
Goya-Maldonado, R
Trost, S
Kramer, B
Keil, M
Binder, EB
Gruber, O
AF Wolf, Claudia
Mohr, Holger
Diekhof, Esther K.
Vieker, Henning
Goya-Maldonado, Roberto
Trost, Sarah
Kraemer, Bernd
Keil, Maria
Binder, Elisabeth B.
Gruber, Oliver
TI CREB1 Genotype Modulates Adaptive Reward-Based Decisions in Humans
SO CEREBRAL CORTEX
LA English
DT Article
DE CREB1; decision-making; fMRI; genetics; reward
ID ELEMENT-BINDING PROTEIN; RESIST IMPULSIVE DESIRES; NUCLEUS-ACCUMBENS;
FEAR MEMORY; BEHAVIORAL-RESPONSES; PREFRONTAL CORTEX; NEURAL MECHANISMS;
EMOTIONAL STIMULI; MAJOR DEPRESSION; BIPOLAR DISORDER
AB Cyclic AMP response element-binding protein (CREB) contributes to adaptation of mesocorticolimbic networks by modulating activity-regulated transcription and plasticity in neurons. Activity or expression changes of CREB in the nucleus accumbens (NAc) and orbital frontal cortex (OFC) interact with behavioral changes during reward-motivated learning. However, these findings from animal models have not been evaluated in humans. We tested whether CREB1 genotypes affect reward-motivated decisions and related brain activation, using BOLD fMRI in 224 young and healthy participants. More specifically, participants needed to adapt their decision to either pursue or resist immediate rewards to optimize the reward outcome. We found significant CREB1 genotype effects on choices to pursue increases of the reward outcome and on BOLD signal in the NAc, OFC, insula cortex, cingulate gyrus, hippocampus, amygdala, and precuneus during these decisions in comparison with those decisions avoiding total reward loss. Our results suggest that CREB1 genotype effects in these regions could contribute to individual differences in reward-and associative memory-based decision-making.
C1 [Wolf, Claudia; Mohr, Holger; Diekhof, Esther K.; Vieker, Henning; Goya-Maldonado, Roberto; Trost, Sarah; Kraemer, Bernd; Keil, Maria; Gruber, Oliver] Univ Gottingen, Dept Psychiat & Psychotherapy, Ctr Translat Res Syst Neurosci & Psychiat, D-37075 Gottingen, Germany.
[Wolf, Claudia] NIA, Lab Behav Neurosci, Baltimore, MD 21224 USA.
[Mohr, Holger] Tech Univ Dresden, Dept Gen Psychol, D-01069 Dresden, Germany.
[Diekhof, Esther K.] Univ Hamburg, Grindel Bioctr, D-20146 Hamburg, Germany.
[Diekhof, Esther K.] Univ Hamburg, Inst Humanbiol, Zool Museum, D-20146 Hamburg, Germany.
[Vieker, Henning] Univ Med Ctr Hamburg Eppendorf, Dept Psychiat & Psychotherapy, D-20246 Hamburg, Germany.
[Binder, Elisabeth B.] Max Planck Inst Psychiat, D-80804 Munich, Germany.
RP Wolf, C (reprint author), Univ Gottingen, Dept Psychiat & Psychotherapy, Ctr Translat Res Syst Neurosci & Psychiat, D-37075 Gottingen, Germany.; Wolf, C (reprint author), NIA, Lab Behav Neurosci, Baltimore, MD 21224 USA.
EM claudia.wolf@nih.gov
FU NIH Intramural Research Program
FX C.W. was partly supported by the NIH Intramural Research Program.
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PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1047-3211
EI 1460-2199
J9 CEREB CORTEX
JI Cereb. Cortex
PD JUL
PY 2016
VL 26
IS 7
BP 2970
EP 2981
DI 10.1093/cercor/bhv104
PG 12
WC Neurosciences
SC Neurosciences & Neurology
GA DV8QC
UT WOS:000383200500003
PM 26045569
ER
PT J
AU Lee, NR
Adeyemi, EI
Lin, A
Clasen, LS
Lalonde, FM
Condon, E
Driver, DI
Shaw, P
Gogtay, N
Raznahan, A
Giedd, JN
AF Lee, Nancy Raitano
Adeyemi, Elizabeth I.
Lin, Amy
Clasen, Liv S.
Lalonde, Franois M.
Condon, Ellen
Driver, David I.
Shaw, Philip
Gogtay, Nitin
Raznahan, Armin
Giedd, Jay N.
TI Dissociations in Cortical Morphometry in Youth with Down Syndrome:
Evidence for Reduced Surface Area but Increased Thickness
SO CEREBRAL CORTEX
LA English
DT Article
DE Alzheimer's disease; cerebral cortex; intellectual disability; magnetic
resonance imaging; Trisomy 21
ID AUTOMATED 3-D EXTRACTION; HUMAN CEREBRAL-CORTEX; HIGH-RESOLUTION MRI;
FRAGILE-X-SYNDROME; SHORT-TERM-MEMORY; DEVELOPMENTAL TRAJECTORIES;
AMYLOID DEPOSITION; ALZHEIMERS-DISEASE; BRAIN; CHILDREN
AB Detailed descriptions of cortical anatomy in youth with Down syndrome (DS), the most common genetic cause of intellectual disability (ID), are scant. Thus, the current study examined deviations in cortical thickness (CT) and surface area (SA), at high spatial resolution, in youth with DS, to identify focal differences relative to typically developing (TD) youth. Participants included 31 youth with DS and 45 age-and sex-matched TD controls (mean age similar to 16 years; range = 5-24 years). All participants completed T-1-weighted ASSET-calibrated magnetization prepared rapid gradient echo scans on a 3-T magnetic resonance imaging scanner. Replicating prior investigations, cortical volume was reduced in DS compared with controls. However, a novel dissociation for SA and CT was found-namely, SA was reduced (predominantly in frontal and temporal regions) while CT was increased (notably in several regions thought to belong to the default mode network; DMN). These findings suggest that reductions in SA rather than CT are driving the cortical volume reductions reported in prior investigations of DS. Moreover, given the link between DMN functionality and Alzheimer's symptomatology in chromosomally typical populations, future DS studies may benefit from focusing on the cortex in DMN regions, as such investigations may provide clues to the precocious onset of Alzheimer's disease in this at-risk group.
C1 [Lee, Nancy Raitano; Adeyemi, Elizabeth I.; Lin, Amy; Clasen, Liv S.; Lalonde, Franois M.; Driver, David I.; Gogtay, Nitin; Raznahan, Armin; Giedd, Jay N.] NIMH, Child Psychiat Branch, NIH, Bethesda, MD 20892 USA.
[Condon, Ellen] NIMH, Funct MRI Core Facil, NIH, Bethesda, MD 20892 USA.
[Lee, Nancy Raitano] Drexel Univ, Dept Psychol, 3141 Chestnut St,Suite 119, Philadelphia, PA 19103 USA.
[Shaw, Philip] Natl Human Genome Res Inst, Social & Behav Res Branch, NIH, Bethesda, MD 20892 USA.
[Giedd, Jay N.] Univ Calif San Diego, Dept Psychiat, La Jolla, CA 92093 USA.
RP Lee, NR (reprint author), Drexel Univ, Dept Psychol, 3141 Chestnut St,Suite 119, Philadelphia, PA 19103 USA.
EM nrl39@drexel.edu
RI Lee, Nancy/M-7492-2016
OI Lee, Nancy/0000-0002-6663-0713
FU Intramural Research Program of the National Institutes of Health,
National Institute of Mental Health [1 ZIA MH002794-13, 89-M-0006]
FX This work was supported by the Intramural Research Program of the
National Institutes of Health, National Institute of Mental Health (1
ZIA MH002794-13; Protocol ID 89-M-0006).
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PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1047-3211
EI 1460-2199
J9 CEREB CORTEX
JI Cereb. Cortex
PD JUL
PY 2016
VL 26
IS 7
BP 2982
EP 2990
DI 10.1093/cercor/bhv107
PG 9
WC Neurosciences
SC Neurosciences & Neurology
GA DV8QC
UT WOS:000383200500004
PM 26088974
ER
PT J
AU Tomasi, D
Shokri-Kojori, E
Volkow, ND
AF Tomasi, Dardo
Shokri-Kojori, Ehsan
Volkow, Nora D.
TI High-Resolution Functional Connectivity Density: Hub Locations,
Sensitivity, Specificity, Reproducibility, and Reliability
SO CEREBRAL CORTEX
LA English
DT Article
DE aging; FCDM; global signal regression; motion; multiband; physiologic
noise; resting state
ID GLOBAL SIGNAL REGRESSION; HUMAN CONNECTOME PROJECT; TEST-RETEST
RELIABILITY; INDEPENDENT COMPONENT ANALYSIS; ANTI-CORRELATED NETWORKS;
RESTING STATE NETWORKS; HUMAN BRAIN; SMALL-WORLD; ALZHEIMERS-DISEASE;
NEURONAL-ACTIVITY
AB Brain regions with high connectivity have high metabolic cost and their disruption is associated with neuropsychiatric disorders. Prior neuroimaging studies have identified at the group-level local functional connectivity density (lFCD) hubs, network nodes with high degree of connectivity with neighboring regions, in occipito-parietal cortices. However, the individual patterns and the precision for the location of the hubs were limited by the restricted spatiotemporal resolution of the magnetic resonance imaging (MRI) measures collected at rest. In this work, we show that MRI datasets with higher spatiotemporal resolution (2-mm isotropic; 0.72 s), collected under the Human Connectome Project (HCP), provide a significantly higher precision for hub localization and for the first time reveal lFCD patterns with gray matter (GM) specificity >96% and sensitivity >75%. High temporal resolution allowed effective 0.01-0.08 Hz band-pass filtering, significantly reducing spurious lFCD effects in white matter. These high spatiotemporal resolution lFCD measures had high reliability [intraclass correlation, ICC(3,1) > 0.6] but lower reproducibility (>67%) than the low spatiotemporal resolution equivalents. GM sensitivity and specificity benchmarks showed the robustness of lFCD to changes in model parameter and preprocessing steps. Mapping individual's brain hubs with high sensitivity, specificity, and reproducibility supports the use of lFCD as a biomarker for clinical applications in neuropsychiatric disorders.
C1 [Tomasi, Dardo; Shokri-Kojori, Ehsan; Volkow, Nora D.] NIAAA, Bethesda, MD USA.
[Volkow, Nora D.] NIDA, Bethesda, MD 20892 USA.
RP Tomasi, D (reprint author), 10 Ctr Dr,Rm B2L124, Bethesda, MD 20892 USA.
EM dardo.tomasi@nih.gov
FU Human Connectome Project, WU-Minn Consortium - 16 NIH Institutes and
Centers [1U54MH091657]; McDonnell Center for Systems Neuroscience at
Washington University; National Institutes of Alcohol Abuse and
Alcoholism [2RO1AA09481]
FX Data were provided by the Human Connectome Project, WU-Minn Consortium
(Principal Investigators: David Van Essen and Kamil Ugurbil;
1U54MH091657) funded by the 16 NIH Institutes and Centers that support
the NIH Blueprint for Neuroscience Research and by the McDonnell Center
for Systems Neuroscience at Washington University. This work was
accomplished with support from the National Institutes of Alcohol Abuse
and Alcoholism (2RO1AA09481).
NR 73
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PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1047-3211
EI 1460-2199
J9 CEREB CORTEX
JI Cereb. Cortex
PD JUL
PY 2016
VL 26
IS 7
BP 3249
EP 3259
DI 10.1093/cercor/bhv171
PG 11
WC Neurosciences
SC Neurosciences & Neurology
GA DV8QC
UT WOS:000383200500025
PM 26223259
ER
PT J
AU Damiano, D
Forssberg, H
AF Damiano, Diane
Forssberg, Hans
TI We are the world: meeting the global challenge of childhood disability
SO DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY
LA English
DT Editorial Material
C1 [Damiano, Diane] NIH, Dept Rehabil Med, Bethesda, MD 20892 USA.
[Forssberg, Hans] Karolinska Inst, Astrid Lindgren Childrens Hosp, Neuropaediat, Stockholm, Sweden.
RP Damiano, D (reprint author), NIH, Dept Rehabil Med, Bethesda, MD 20892 USA.
NR 0
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PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0012-1622
EI 1469-8749
J9 DEV MED CHILD NEUROL
JI Dev. Med. Child Neurol.
PD JUL
PY 2016
VL 58
IS 7
BP 649
EP 649
DI 10.1111/dmcn.13154
PG 1
WC Clinical Neurology; Pediatrics
SC Neurosciences & Neurology; Pediatrics
GA DV3VZ
UT WOS:000382854000002
PM 27307194
ER
PT J
AU Ghassabian, A
Sundaram, R
Wylie, A
Bell, E
Bello, SC
Yeung, E
AF Ghassabian, Akhgar
Sundaram, Rajeshwari
Wylie, Amanda
Bell, Erin
Bello, Scott C.
Yeung, Edwina
TI Maternal medical conditions during pregnancy and gross motor development
up to age 24 months in the Upstate KIDS study
SO DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY
LA English
DT Article
ID GESTATIONAL DIABETES-MELLITUS; DNA METHYLATION; CHILDREN BORN; BIRTH
COHORT; INFANTS BORN; SCHOOL-AGE; MOTHERS; POPULATION; MILESTONES;
OBESITY
AB AIM We examined whether children of mothers with a medical condition diagnosed before or during pregnancy took longer to achieve gross motor milestones up to age 24 months.
METHOD We obtained information on medical conditions using self-reports, birth certificates, and hospital records in 4909 mothers participating in Upstate KIDS, a population-based birth cohort. Mothers reported on their children's motor milestone achievement at 4, 8, 12, 18, and 24 months of age.
RESULTS After adjustment for covariates (including pre-pregnancy body mass index), children of mothers with gestational diabetes took longer to achieve sitting without support (hazard ratio [HR]=0.84, 95% confidence interval [CI] 0.75-0.93), walking with assistance (HR=0.88, 95% CI 0.77-0.98), and walking alone (HR=0.88, 95% CI 0.77-0.99) than children of females with no gestational diabetes. Similar findings emerged for maternal diabetes. Gestational hypertension was associated with a longer time to achieve walking with assistance. These associations did not change after adjustment for gestational age or birthweight. Severe hypertensive disorders of pregnancy were related to a longer time to achieve milestones, but not after adjustment for perinatal factors.
INTERPRETATION Children exposed to maternal diabetes, gestational or pre-gestational, may take longer to achieve motor milestones than non-exposed children, independent of maternal obesity.
C1 [Ghassabian, Akhgar; Wylie, Amanda; Yeung, Edwina] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Epidemiol Branch, Div Intramural Populat Hlth Res, NIH, Bethesda, MD 20892 USA.
[Sundaram, Rajeshwari] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Biostat & Bioinformat Branch, Div Intramural Populat Hlth Res, NIH, Bethesda, MD 20892 USA.
[Bell, Erin] SUNY Albany, Sch Publ Hlth, Dept Environm Hlth Sci, Albany, NY 12222 USA.
[Bell, Erin] SUNY Albany, Sch Publ Hlth, Dept Epidemiol & Biostat, Albany, NY 12222 USA.
[Bello, Scott C.] CapitalCare Pediat Troy, Troy, NY USA.
RP Yeung, E (reprint author), 6100 Execut Blvd,7B03, Bethesda, MD 20892 USA.
EM yeungedw@mail.nih.gov
OI Ghassabian, Akhgar/0000-0001-9551-4706; Yeung,
Edwina/0000-0002-3851-2613; Sundaram, Rajeshwari/0000-0002-6918-5002
FU Intramural Research Program of the Eunice Kennedy Shriver National
Institute of Child Health and Human Development (NICHD)
[HHSN275201200005C, HHSN267200700019C]
FX Supported by the Intramural Research Program of the Eunice Kennedy
Shriver National Institute of Child Health and Human Development (NICHD;
contracts #HHSN275201200005C, #HHSN267200700019C). The authors thank all
the Upstate KIDS families and staff for their important contributions.
The funders had no role in the study design, data collection, analysis,
interpretation of the data, or writing of the report. The corresponding
author (EY) had full access to all the data in the study and takes
responsibility for the integrity of the data and the accuracy of the
data analysis. The authors have stated that they had no interests that
might be perceived as posing a conflict or bias.
NR 25
TC 0
Z9 0
U1 2
U2 2
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0012-1622
EI 1469-8749
J9 DEV MED CHILD NEUROL
JI Dev. Med. Child Neurol.
PD JUL
PY 2016
VL 58
IS 7
BP 728
EP 734
DI 10.1111/dmcn.12947
PG 7
WC Clinical Neurology; Pediatrics
SC Neurosciences & Neurology; Pediatrics
GA DV3VZ
UT WOS:000382854000022
PM 26502927
ER
PT J
AU Iranzo, J
Krupovic, M
Koonin, EV
AF Iranzo, Jaime
Krupovic, Mart
Koonin, Eugene V.
TI The Double-Stranded DNA Virosphere as a Modular Hierarchical Network of
Gene Sharing
SO MBIO
LA English
DT Article
ID PROTEIN-INTERACTION NETWORK; ARCHAEAL VIRUSES; GENOME EVOLUTION; DSDNA
BACTERIOPHAGES; REPLICATOR SYSTEMS; ORTHOLOGOUS GENES; CAPSID PROTEINS;
MARINE VIRUSES; PSI-BLAST; TELL US
AB Virus genomes are prone to extensive gene loss, gain, and exchange and share no universal genes. Therefore, in a broad-scale study of virus evolution, gene and genome network analyses can complement traditional phylogenetics. We performed an exhaustive comparative analysis of the genomes of double-stranded DNA (dsDNA) viruses by using the bipartite network approach and found a robust hierarchical modularity in the dsDNA virosphere. Bipartite networks consist of two classes of nodes, with nodes in one class, in this case genomes, being connected via nodes of the second class, in this case genes. Such a network can be partitioned into modules that combine nodes from both classes. The bipartite network of dsDNA viruses includes 19 modules that form 5 major and 3 minor supermodules. Of these modules, 11 include tailed bacteriophages, reflecting the diversity of this largest group of viruses. The module analysis quantitatively validates and refines previously proposed nontrivial evolutionary relationships. An expansive supermodule combines the large and giant viruses of the putative order "Megavirales" with diverse moderate-sized viruses and related mobile elements. All viruses in this supermodule share a distinct morphogenetic tool kit with a double jelly roll major capsid protein. Herpesviruses and tailed bacteriophages comprise another supermodule, held together by a distinct set of morphogenetic proteins centered on the HK97-like major capsid protein. Together, these two supermodules cover the great majority of currently known dsDNA viruses. We formally identify a set of 14 viral hallmark genes that comprise the hubs of the network and account for most of the intermodule connections.
IMPORTANCE Viruses and related mobile genetic elements are the dominant biological entities on earth, but their evolution is not sufficiently understood and their classification is not adequately developed. The key reason is the characteristic high rate of virus evolution that involves not only sequence change but also extensive gene loss, gain, and exchange. Therefore, in the study of virus evolution on a large scale, traditional phylogenetic approaches have limited applicability and have to be complemented by gene and genome network analyses. We applied state-of-the art methods of such analysis to reveal robust hierarchical modularity in the genomes of double-stranded DNA viruses. Some of the identified modules combine highly diverse viruses infecting bacteria, archaea, and eukaryotes, in support of previous hypotheses on direct evolutionary relationships between viruses from the three domains of cellular life. We formally identify a set of 14 viral hallmark genes that hold together the genomic network.
C1 [Iranzo, Jaime; Koonin, Eugene V.] Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA.
[Krupovic, Mart] Inst Pasteur, Unite Biol Mol Gene Chez Extremophiles, Paris, France.
RP Koonin, EV (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA.
EM koonin@ncbi.nlm.nih.gov
RI Krupovic, Mart/I-4209-2012; Iranzo, Jaime/H-5197-2011
OI Krupovic, Mart/0000-0001-5486-0098; Iranzo, Jaime/0000-0002-4538-7726
FU US Department of Health and Human Services
FX This work was funded by US Department of Health and Human Services
(Intramural funds).
NR 101
TC 3
Z9 3
U1 6
U2 6
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e00978
DI 10.1128/mBio.00978-16
PG 21
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500044
ER
PT J
AU Kohler, LJ
Reed, SR
Sarraf, SA
Arteaga, DD
Newton, HJ
Roy, CR
AF Kohler, Lara J.
Reed, Shawna R.
Sarraf, Shireen A.
Arteaga, David D.
Newton, Hayley J.
Roy, Craig R.
TI Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing
Constitutive Fusion of Autophagosomes with the Coxiella-Containing
Vacuole
SO MBIO
LA English
DT Article
ID INTRACELLULAR REPLICATION; PARASITOPHOROUS VACUOLE; GALLERIA-MELLONELLA;
AXENIC GROWTH; CELL-DEATH; PHASE-I; BURNETII; SECRETION; PATHWAY;
IDENTIFICATION
AB Coxiella burnetii replicates in an acidified lysosome-derived vacuole. Biogenesis of the Coxiella-containing vacuole (CCV) requires bacterial effector proteins delivered into host cells by the Dot/Icm secretion system. Genetic and cell biological analysis revealed that an effector protein called Cig2 promotes constitutive fusion of autophagosomes with the CCV to maintain this compartment in an autolysosomal stage of maturation. This distinguishes the CCV from other pathogen-containing vacuoles that are targeted by the host autophagy pathway, which typically confers host resistance to infection by delivering the pathogen to a toxic lysosomal environment. By maintaining the CCV in an autolysosomal stage of maturation, Cig2 enabled CCV homotypic fusion and enhanced bacterial virulence in the Galleria mellonella (wax moth) model of infection by a mechanism that decreases host tolerance. Thus, C. burnetii residence in an autolysosomal organelle alters host tolerance of infection, which indicates that Cig2-dependent manipulation of a lysosome-derived vacuole influences the host response to infection.
IMPORTANCE Coxiella burnetii is an obligate, intracellular bacterial pathogen that replicates inside a unique, lysosome-like compartment called the Coxiella-containing vacuole (CCV). Over 130 bacterial effector proteins are delivered into the host cell cytosol by the C. burnetii Dot/Icm type IV secretion system. Although the Dot/Icm system is essential for pathogenesis, the functions of most effectors remain unknown. Here we show that the effector protein Cig2 is essential for converting the CCV to an organelle that is similar to the autolysosome. Cig2 function promotes constitutive fusion between the CCV and autophagosomes generated by selective autophagy. Cig2-directed biogenesis of an autolysosomal vacuole is essential for the unique fusogenic properties of the CCV and for virulence in an animal model of disease. This work highlights how bacterial subversion of the host autophagy pathway can influence the cell biological properties of the CCV and influence the host response to infection.
C1 [Kohler, Lara J.; Reed, Shawna R.; Arteaga, David D.; Roy, Craig R.] Yale Univ, Sch Med, Dept Microbial Pathogenesis, Boyer Ctr Mol Med, New Haven, CT 06510 USA.
[Sarraf, Shireen A.] NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
[Newton, Hayley J.] Univ Melbourne, Dept Microbiol & Immunol, Peter Doherty Inst Infect & Immun, Melbourne, Vic, Australia.
RP Roy, CR (reprint author), Yale Univ, Sch Med, Dept Microbial Pathogenesis, Boyer Ctr Mol Med, New Haven, CT 06510 USA.
EM craig.roy@yale.edu
OI Newton, Hayley/0000-0002-9240-2001
FU HHS \ National Institutes of Health (NIH) [R01 AI114760, F32 AI114196];
National Science Foundation (NSF)
FX This work, including the efforts of Craig R Roy, was funded by HHS
vertical bar National Institutes of Health (NIH) (R01 AI114760). This
work, including the efforts of Shawna R Reed, was funded by HHS vertical
bar National Institutes of Health (NIH) (F32 AI114196). This work,
including the efforts of Lara J Kohler, was funded by National Science
Foundation (NSF) (Pre Doctoral Fellowship).
NR 53
TC 1
Z9 1
U1 1
U2 1
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e01127
DI 10.1128/mBio.01127-16
PG 14
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500062
ER
PT J
AU Kohler, LJ
Reed, SCO
Sarraf, SA
Arteaga, DD
Newton, HJ
Roy, CR
AF Kohler, Lara J.
Reed, Shawna C. O.
Sarraf, Shireen A.
Arteaga, David D.
Newton, Hayley J.
Roy, Craig R.
TI Effector Protein Cig2 Decreases Host Tolerance of Infection by Directing
Constitutive Fusion of Autophagosomes with the Coxiella-Containing
Vacuole (vol 7, e01127, 2016)
SO MBIO
LA English
DT Correction
C1 [Kohler, Lara J.; Reed, Shawna C. O.; Arteaga, David D.; Roy, Craig R.] Yale Univ, Sch Med, Boyer Ctr Mol Med, Dept Microbial Pathogenesis, New Haven, CT 06510 USA.
[Sarraf, Shireen A.] NINDS, Biochem Sect, Surg Neurol Branch, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
[Newton, Hayley J.] Univ Melbourne, Peter Doherty Inst Infect & Immun, Dept Microbiol & Immunol, Melbourne, Vic, Australia.
RP Roy, CR (reprint author), Yale Univ, Sch Med, Boyer Ctr Mol Med, Dept Microbial Pathogenesis, New Haven, CT 06510 USA.
EM craig.roy@yale.edu
NR 1
TC 1
Z9 1
U1 0
U2 0
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e01327
DI 10.1128/mBio.01327-16
PG 1
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500063
ER
PT J
AU Ntumngia, FB
Thomson-Luque, R
Torres, LD
Gunalan, K
Carvalho, LH
Adams, JH
AF Ntumngia, Francis B.
Thomson-Luque, Richard
Torres, Leticia de Menezes
Gunalan, Karthigayan
Carvalho, Luzia H.
Adams, John H.
TI A Novel Erythrocyte Binding Protein of Plasmodium vivax Suggests an
Alternate Invasion Pathway into Duffy-Positive Reticulocytes
SO MBIO
LA English
DT Article
ID BLOOD-GROUP; MALARIA PARASITES; ANTIBODIES; FALCIPARUM; INFECTION;
AFRICANS; RECEPTOR; REVEALS; DOMAINS; ANTIGEN
AB Erythrocyte invasion by malaria parasites is essential for blood-stage development and an important determinant of host range. In Plasmodium vivax, the interaction between the Duffy binding protein (DBP) and its cognate receptor, the Duffy antigen receptor for chemokines (DARC), on human erythrocytes is central to blood-stage infection. Contrary to this established pathway of invasion, there is growing evidence of P. vivax infections occurring in Duffy blood group-negative individuals, suggesting that the parasite might have gained an alternative pathway to infect this group of individuals. Supporting this concept, a second distinct erythrocyte binding protein (EBP2), representing a new member of the DBP family, was discovered in P. vivax and may be the ligand in an alternate invasion pathway. Our study characterizes this novel ligand and determines its potential role in reticulocyte invasion by P. vivax merozoites. EBP2 binds preferentially to young (CD71(high)) Duffy-positive (Fy(+)) reticulocytes and has minimal binding capacity for Duffy-negative reticulocytes. Importantly, EBP2 is antigenically distinct from DBP and cannot be functionally inhibited by anti-DBP antibodies. Consequently, our results do not support EBP2 as a ligand for invasion of Duffy-negative blood cells, but instead, EBP2 may represent a novel ligand for an alternate invasion pathway of Duffypositive reticulocytes.
IMPORTANCE For decades, P. vivax infections in humans have been defined by a unique requirement for the interaction between the Duffy binding protein ligand of the parasite and the Duffy blood group antigen receptor (DARC). Recent reports of P. vivax infections in Duffy-negative individuals challenge this paradigm and suggest an alternate pathway of infection, potentially using the recently discovered EBP2. However, we demonstrate that EBP2 host cell specificity is more restricted than DBP binding and that EBP2 binds preferentially to Duffy-positive, young reticulocytes. This finding indicates that this DBP paralog does mediate a Duffy-independent pathway of infection.
C1 [Ntumngia, Francis B.; Thomson-Luque, Richard; Torres, Leticia de Menezes; Adams, John H.] Univ S Florida, Coll Publ Hlth, Ctr Global Hlth & Infect Dis Res, Tampa, FL 33620 USA.
[Torres, Leticia de Menezes; Carvalho, Luzia H.] Fiocruz MS, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil.
[Gunalan, Karthigayan] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD USA.
RP Ntumngia, FB; Adams, JH (reprint author), Univ S Florida, Coll Publ Hlth, Ctr Global Hlth & Infect Dis Res, Tampa, FL 33620 USA.
EM fntumngi@health.usf.edu; usfmalaria@gmail.com
RI Adams, John/G-1800-2015
OI Adams, John/0000-0003-3707-7979
FU HHS \ National Institutes of Health (NIH) [R01AI064478, R21AI107455];
Brazilian National Council of Technological and Scientific
Development-CNPq [249764/2013-0]
FX This work, including the efforts of John H Adams, was funded by HHS
vertical bar National Institutes of Health (NIH) (R01AI064478). This
work, including the efforts of Francis B Ntumngia, was funded by HHS
vertical bar National Institutes of Health (NIH) (R21AI107455). This
work, including the efforts of Leticia de Menezes Torres, was funded by
Brazilian National Council of Technological and Scientific
Development-CNPq for a visiting PhD student fellowship (249764/2013-0).
NR 30
TC 1
Z9 1
U1 2
U2 2
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e01261
DI 10.1128/mBio.01261-16
PG 5
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500079
ER
PT J
AU Olsen, ME
Filone, CM
Rozelle, D
Mire, CE
Agans, KN
Hensley, L
Connor, JH
AF Olsen, Michelle E.
Filone, Claire Marie
Rozelle, Dan
Mire, Chad E.
Agans, Krystle N.
Hensley, Lisa
Connor, John H.
TI Polyamines and Hypusination Are Required for Ebolavirus Gene Expression
and Replication
SO MBIO
LA English
DT Article
ID INITIATION-FACTOR 5A; CHLORELLA-VIRUS PBCV-1; NIEMANN-PICK C1; PROMOTES
TRANSLATION; RNA SYNTHESIS; AMINO-ACID; INFECTION; DECARBOXYLASE;
INHIBITION; CELLS
AB Ebolavirus (EBOV) is an RNA virus that is known to cause severe hemorrhagic fever in humans and other primates. EBOV successfully enters and replicates in many cell types. This replication is dependent on the virus successfully coopting a number of cellular factors. Many of these factors are currently unidentified but represent potential targets for antiviral therapeutics. Here we show that cellular polyamines are critical for EBOV replication. We found that small-molecule inhibitors of polyamine synthesis block gene expression driven by the viral RNA-dependent RNA polymerase. Short hairpin RNA (shRNA) knockdown of the polyamine pathway enzyme spermidine synthase also resulted in reduced EBOV replication. These findings led us to further investigate spermidine, a polyamine that is essential for the hypusination of eukaryotic initiation factor 5A (eIF5A). Blocking the hypusination of eIF5A (and thereby inhibiting its function) inhibited both EBOV gene expression and viral replication. The mechanism appears to be due to the importance of hypusinated eIF5A for the accumulation of VP30, an essential component of the viral polymerase. The same reduction in hypusinated eIF5A did not alter the accumulation of other viral polymerase components. This action makes eIF5A function an important gate for proper EBOV polymerase assembly and function through the control of a single virus protein.
IMPORTANCE Ebolavirus (EBOV) is one of the most lethal human pathogens known. EBOV requires host factors for replication due to its small RNA genome. Here we show that the host protein eIF5A in its activated form is necessary for EBOV replication. We further show that the mechanism is through the accumulation of a single EBOV protein, VP30. To date, no other host proteins have been shown to interfere with the translation or stability of an EBOV protein. Activated eIF5A is the only protein in the cell known to contain the specific modification of hypusine; therefore, this pathway is a target for drug development. Further investigation into the mechanism of eIF5A interaction with VP30 could provide insight into therapeutics to combat EBOV.
C1 [Olsen, Michelle E.; Filone, Claire Marie; Rozelle, Dan; Connor, John H.] Boston Univ, Dept Microbiol, Boston, MA 02215 USA.
[Mire, Chad E.; Agans, Krystle N.] Univ Texas Med Branch, Galveston Natl Lab, Galveston, TX 77555 USA.
[Hensley, Lisa] US Army Med Res Inst Infect Dis, Ft Detrick, MD USA.
[Hensley, Lisa] NIAID, Integrated Res Facil, NIH, Ft Detrick, MD USA.
[Filone, Claire Marie] Natl Biodef Anal & Countermeasures Ctr, Ft Detrick, MD USA.
[Rozelle, Dan] Pfizer Inc, Andover, MA USA.
RP Connor, JH (reprint author), Boston Univ, Dept Microbiol, Boston, MA 02215 USA.
EM jhconnor@bu.edu
OI Connor, John/0000-0002-8867-7256
FU HHS \ National Institutes of Health (NIH) [AI121933, AI1096159-04,
UC7-AI094660]; HHS \ NIH \ National Institute of Allergy and Infectious
Diseases (NIAID) [UC6AI058618]
FX This work, including the efforts of John Connor, Michelle E. Olsen,
Claire Marie Filone, and Dan Rozelle, was funded by HHS vertical bar
National Institutes of Health (NIH) (AI121933 and AI1096159-04). This
work, including the efforts of Chad Mire and Krystle Agans, was funded
by HHS vertical bar National Institutes of Health (NIH) (UC7-AI094660).
This work, including the efforts of John Connor, Michelle E. Olsen,
Claire Marie Filone, and Dan Rozelle, was funded by HHS vertical bar NIH
vertical bar National Institute of Allergy and Infectious Diseases
(NIAID) (UC6AI058618).
NR 36
TC 2
Z9 2
U1 1
U2 1
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e00882-16
DI 10.1128/mBio.00882-16
PG 10
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500033
ER
PT J
AU Park, YD
Sun, W
Salas, A
Antia, A
Carvajal, C
Wang, A
Xu, X
Meng, ZJ
Zhou, M
Tawa, GJ
Dehdashti, J
Zheng, W
Henderson, CM
Zelazny, AM
Williamson, PR
AF Park, Yoon-Dong
Sun, Wei
Salas, Antonio
Antia, Avan
Carvajal, Cindy
Wang, Amy
Xu, Xin
Meng, Zhaojin
Zhou, Ming
Tawa, Gregory J.
Dehdashti, Jean
Zheng, Wei
Henderson, Christina M.
Zelazny, Adrian M.
Williamson, Peter R.
TI Identification of Multiple Cryptococcal Fungicidal Drug Targets by
Combined Gene Dosing and Drug Affinity Responsive Target Stability
Screening
SO MBIO
LA English
DT Article
ID VACUOLAR H+-ATPASE; AMPHOTERICIN-B; DEHYDROGENASE; NEOFORMANS;
MENINGITIS; BITHIONOL; PARAGONIMIASIS; SIMILARITIES; METABOLITES;
INHIBITORS
AB Cryptococcus neoformans is a pathogenic fungus that is responsible for up to half a million cases of meningitis globally, especially in immunocompromised individuals. Common fungistatic drugs, such as fluconazole, are less toxic for patients but have low efficacy for initial therapy of the disease. Effective therapy against the disease is provided by the fungicidal drug amphotericin B; however, due to its high toxicity and the difficulty in administering its intravenous formulation, it is imperative to find new therapies targeting the fungus. The antiparasitic drug bithionol has been recently identified as having potent fungicidal activity. In this study, we used a combined gene dosing and drug affinity responsive target stability (GD-DARTS) screen as well as protein modeling to identify a common drug binding site of bithionol within multiple NAD-dependent dehydrogenase drug targets. This combination genetic and proteomic method thus provides a powerful method for identifying novel fungicidal drug targets for further development.
IMPORTANCE Cryptococcosis is a neglected fungal meningitis that causes approximately half a million deaths annually. The most effective antifungal agent, amphotericin B, was developed in the 1950s, and no effective medicine has been developed for this disease since that time. A key aspect of amphotericin B's effectiveness is thought to be because of its ability to kill the fungus (fungicidal activity), rather than just stop or slow its growth. The present study utilized a recently identified fungicidal agent, bithionol, to identify potential fungicidal drug targets that can be used in developing modern fungicidal agents. A combined protein and genetic analysis approach was used to identify a class of enzymes, dehydrogenases, that the fungus uses to maintain homeostasis with regard to sugar nutrients. Similarities in the drug target site were found that resulted in simultaneous inhibition and killing of the fungus by bithionol. These studies thus identify a common, multitarget site for antifungal development.
C1 [Park, Yoon-Dong; Salas, Antonio; Antia, Avan; Carvajal, Cindy; Williamson, Peter R.] NIAID, Lab Clin Infect Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
[Sun, Wei; Wang, Amy; Xu, Xin; Tawa, Gregory J.; Dehdashti, Jean; Zheng, Wei] NIH, Natl Ctr Adv Translat Sci, Bldg 10, Bethesda, MD 20892 USA.
[Meng, Zhaojin; Zhou, Ming] Frederick Natl Lab Canc Res, Prot Characterizat Lab, Canc Res Technol Program, Frederick, MD USA.
[Henderson, Christina M.; Zelazny, Adrian M.] NIH, Microbiol Serv, Dept Lab Med, Ctr Clin, Bldg 10, Bethesda, MD 20892 USA.
[Williamson, Peter R.] Univ Illinois, Coll Med, Dept Med, Infect Dis Sect, Chicago, IL 60612 USA.
RP Williamson, PR (reprint author), NIAID, Lab Clin Infect Dis, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.; Williamson, PR (reprint author), Univ Illinois, Coll Med, Dept Med, Infect Dis Sect, Chicago, IL 60612 USA.
EM williamsonpr@mail.nih.gov
RI Zheng, Wei/J-8889-2014
OI Zheng, Wei/0000-0003-1034-0757
FU HHS\NIH\ National Institute of Allergy and Infectious Diseases (NIAID)
[AI001123]; HHS \ NIH \ National Institute of Allergy and Infectious
Diseases (NIAID) [AI001124]
FX This work, including the efforts of Peter R Williamson, was funded by
HHS vertical bar NIH vertical bar National Institute of Allergy and
Infectious Diseases (NIAID) (AI001123). This work, including the efforts
of Peter R Williamson, was funded by HHS vertical bar NIH vertical bar
National Institute of Allergy and Infectious Diseases (NIAID)
(AI001124).
NR 49
TC 0
Z9 0
U1 12
U2 12
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e01073
DI 10.1128/mBio.01073-16
PG 11
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500056
ER
PT J
AU Tsetsarkin, KA
Kenney, H
Chen, RB
Liu, GP
Manukyan, H
Whitehead, SS
Laassri, M
Chumakov, K
Pletnev, AG
AF Tsetsarkin, Konstantin A.
Kenney, Heather
Chen, Rubing
Liu, Guangping
Manukyan, Hasmik
Whitehead, Stephen S.
Laassri, Majid
Chumakov, Konstantin
Pletnev, Alexander G.
TI A Full-Length Infectious cDNA Clone of Zika Virus from the 2015 Epidemic
in Brazil as a Genetic Platform for Studies of Virus-Host Interactions
and Vaccine Development
SO MBIO
LA English
DT Article
ID JAPANESE-ENCEPHALITIS-VIRUS; COOPERATIVE INTERACTIONS; RT-PCR; RNA; DNA;
ROBUSTNESS; EVOLUTION; AMERICA; AMPLIFICATION; TRANSMISSION
AB An arthropod-borne virus, Zika virus (ZIKV), has recently emerged as a major human pathogen. Associated with complications during perinatal development and Guillain-Barr syndrome in adults, ZIKV raises new challenges for understanding the molecular determinants of flavivirus pathogenesis. This underscores the necessity for the development of a reverse genetic system based on an epidemic ZIKV strain. Here, we describe the generation and characterization in cell cultures of an infectious cDNA clone of ZIKV isolated from the 2015 epidemic in Brazil. The cDNA-derived ZIKV replicated efficiently in a variety of cell lines, including those of both neuronal and placental origin. We observed that the growth of cDNA-derived virus was attenuated compared to the growth of the parental isolate in most cell lines, which correlates with substantial differences in sequence heterogeneity between these viruses that were determined by deep-sequencing analysis. Our findings support the role of genetic diversity in maintaining the replicative fitness of viral populations under changing conditions. Moreover, these results indicate that caution should be exercised when interpreting the results of reverse-genetics experiments in attempts to accurately predict the biology of natural viruses. Finally, a Vero cell-adapted cDNA clone of ZIKV was generated that can be used as a convenient platform for studies aimed at the development of ZIKV vaccines and therapeutics.
IMPORTANCE The availability of genetic tools and laboratory models determines the progress in understanding mechanisms of virus emergence and pathogenesis. Recent large-scale outbreaks of Zika virus (ZIKV) that were linked to complications during perinatal development and Guillain-Barre syndrome in adults emphasize the urgency for the development of a reverse-genetics system based on an epidemic ZIKV strain. Here, we report a stable infectious cDNA clone for ZIKV isolated during the 2015 epidemic in Brazil, as well as a Vero cell-adapted version of it, which will be used for virus-host interaction studies and vaccine development.
C1 [Tsetsarkin, Konstantin A.; Kenney, Heather; Liu, Guangping; Whitehead, Stephen S.; Pletnev, Alexander G.] NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA.
[Chen, Rubing] Univ Texas Med Branch, Dept Pathol, Inst Human Infect & Immun, Galveston, TX 77555 USA.
[Manukyan, Hasmik; Laassri, Majid; Chumakov, Konstantin] US FDA, Ctr Biol Evaluat & Res, Silver Spring, MD USA.
RP Pletnev, AG (reprint author), NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA.
EM apletnev@niaid.nih.gov
FU Division of Intramural Research Program of the National Institute of
Allergy and Infectious Diseases, National Institutes of Health
FX This work was supported by the Division of Intramural Research Program
of the National Institute of Allergy and Infectious Diseases, National
Institutes of Health
NR 44
TC 10
Z9 10
U1 49
U2 49
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e01114
DI 10.1128/mBio.01114-16
PG 8
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500060
ER
PT J
AU Wu, WM
Leavitt, JC
Cheng, NQ
Gilcrease, EB
Motwani, T
Teschke, CM
Casjens, SR
Steven, AC
AF Wu, Weimin
Leavitt, Justin C.
Cheng, Naiqian
Gilcrease, Eddie B.
Motwani, Tina
Teschke, Carolyn M.
Casjens, Sherwood R.
Steven, Alasdair C.
TI Localization of the Houdinisome (Ejection Proteins) inside the
Bacteriophage P22 Virion by Bubblegram Imaging
SO MBIO
LA English
DT Article
ID COAT PROTEIN; RADIATION-DAMAGE; SALMONELLA PHAGE-P22; I-DOMAIN;
NUCLEOPROTEIN COMPLEXES; 3-DIMENSIONAL STRUCTURE; SCAFFOLDING PROTEIN;
ELECTRON-MICROSCOPY; BINDING DOMAIN; PORTAL VERTEX
AB The P22 capsid is a T=7 icosahedrally symmetric protein shell with a portal protein dodecamer at one 5-fold vertex. Extending outwards from that vertex is a short tail, and putatively extending inwards is a 15-nm-long alpha-helical barrel formed by the C-terminal domains of portal protein subunits. In addition to the densely packed genome, the capsid contains three "ejection proteins" (E-proteins [gp7, gp16, and gp20]) destined to exit from the tightly sealed capsid during the process of DNA delivery into target cells. We estimated their copy numbers by quantitative SDS-PAGE as approximately 12 molecules per virion of gp16 and gp7 and 30 copies of gp20. To localize them, we used bubblegram imaging, an adaptation of cryo-electron microscopy in which gaseous bubbles induced in proteins by prolonged irradiation are used to map the proteins' locations. Weapplied this technique to wild-type P22, a triple mutant lacking all three E-proteins, and three mutants each lacking one E-protein. We conclude that all three E-proteins are loosely clustered around the portal axis, in the region displaced radially inwards from the portal crown. The bubblegram data imply that approximately half of the alpha-helical barrel seen in the portal crystal structure is disordered in the mature virion, and parts of the disordered region present binding sites for E-proteins. Thus positioned, the E-proteins are strategically placed to pass down the shortened barrel and through the portal ring and the tail, as they exit from the capsid during an infection.
IMPORTANCE While it has long been appreciated that capsids serve as delivery vehicles for viral genomes, there is now growing awareness that viruses also deliver proteins into their host cells. P22 has three such proteins (ejection proteins [E-proteins]), whose initial locations in the virion have remained unknown despite their copious amounts (total of 2.5 MDa). This study succeeded in localizing them by the novel technique of bubblegram imaging. The P22 E-proteins are seen to be distributed around the orifice of the portal barrel. Interestingly, this barrel, 15 nm long in a crystal structure, is only about half as long in situ: the remaining, disordered, portion appears to present binding sites for E-proteins. These observations document a spectacular example of a regulatory order-disorder transition in a supramolecular system and demonstrate the potential of bubblegram imaging to map the components of other viruses as well as cellular complexes.
C1 [Wu, Weimin; Cheng, Naiqian; Steven, Alasdair C.] NIAMSD, Struct Biol Res Lab, NIH, Bethesda, MD 20892 USA.
[Leavitt, Justin C.; Gilcrease, Eddie B.; Casjens, Sherwood R.] Univ Utah, Sch Med, Dept Pathol, Div Microbiol & Immunol, Salt Lake City, UT USA.
[Motwani, Tina; Teschke, Carolyn M.] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT USA.
[Teschke, Carolyn M.] Univ Connecticut, Dept Chem, U-60, Storrs, CT USA.
[Leavitt, Justin C.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA.
RP Steven, AC (reprint author), NIAMSD, Struct Biol Res Lab, NIH, Bethesda, MD 20892 USA.
EM stevena@mail.nih.gov
FU HHS \ NIH \ National Institute of General Medical Sciences (NIGMS)
[GM076661, GM114817]; HHS \ NIH \ National Institute of Arthritis and
Musculoskeletal and Skin Diseases (NIAMS) [ZIA AR027002-38]
FX This work, including the efforts of Carolyn M. Teschke, was funded by
HHS vertical bar NIH vertical bar National Institute of General Medical
Sciences (NIGMS) (GM076661). This work, including the efforts of
Sherwood R Casjens, was funded by HHS vertical bar NIH vertical bar
National Institute of General Medical Sciences (NIGMS) (GM114817). This
work, including the efforts of Alasdair C. Steven, was funded by the
intramural research program of HHS vertical bar NIH vertical bar
National Institute of Arthritis and Musculoskeletal and Skin Diseases
(NIAMS) (ZIA AR027002-38).
NR 61
TC 1
Z9 1
U1 5
U2 5
PU AMER SOC MICROBIOLOGY
PI WASHINGTON
PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
SN 2150-7511
J9 MBIO
JI mBio
PD JUL-AUG
PY 2016
VL 7
IS 4
AR e01152
DI 10.1128/mBio.01152-16
PG 10
WC Microbiology
SC Microbiology
GA DW1YQ
UT WOS:000383440500066
ER
PT J
AU Koch, KL
Hasler, WL
Yates, KP
Parkman, HP
Pasricha, PJ
Calles-Escandon, J
Snape, WJ
Abell, TL
Mccallum, RW
Nguyen, LA
Sarosiek, I
Farrugia, G
Tonascia, J
Lee, L
Miriel, L
Hamilton, F
AF Koch, K. L.
Hasler, W. L.
Yates, K. P.
Parkman, H. P.
Pasricha, P. J.
Calles-Escandon, J.
Snape, W. J.
Abell, T. L.
Mccallum, R. W.
Nguyen, L. A.
Sarosiek, I.
Farrugia, G.
Tonascia, J.
Lee, L.
Miriel, L.
Hamilton, F.
CA Niddk Gastroparesis Clinical Res
TI Baseline features and differences in 48 week clinical outcomes in
patients with gastroparesis and type 1 vs type 2 diabetes
SO NEUROGASTROENTEROLOGY AND MOTILITY
LA English
DT Article
DE gastric emptying; gastroparesis; hyperglycemia; nausea and vomiting;
type 1 and type 2 diabetes mellitus
ID UPPER GASTROINTESTINAL DISORDERS; IDIOPATHIC GASTROPARESIS; SYMPTOM
SEVERITY; MELLITUS; PREVALENCE; NAUSEA; DYSFUNCTION; VALIDATION; COHORT;
INDEX
AB Background In studies of diabetic gastroparesis, patients with type 1 and type 2 diabetes mellitus (T1DM, T2DM) are often combined for analyses. We compared gastroparesis severity, healthcare utilization, psychological function, and quality of life in T1DM vs T2DM gastroparesis patients. Methods Questionnaire, laboratory, and scintigraphy data from patients with gastroparesis and T1DM and T2DM from seven centers of the National Institute of Diabetes and Digestive and Kidney Diseases Gastroparesis Clinical Research Consortium Registry were compared at enrollment and after 48 weeks. Multiple regression models assessed baseline and follow-up differences between diabetes subtypes. Key Results At baseline, T1DM patients (N = 78) had slower gastric emptying, more hospitalizations, more gastric stimulator implantations, higher hemoglobin A1c (HbA1c), and more anxiety vs T2DM patients (N = 59). Independent discriminators of patients with T1DM vs T2DM included worse gastroesophageal reflux disease, less bloating, more peripheral neuropathy, and fewer comorbidities (p = 0.05). On follow-up, gastrointestinal (GI) symptom scores decreased only in T2DM (p < 0.05), but not in T1DM patients who reported greater prokinetic, proton pump inhibitor, anxiolytic, and gastric stimulator usage over 48 weeks (p = 0.03). Gastrointestinal symptoms at baseline and 48 weeks with both subtypes were not associated with HbA1c, peripheral neuropathy, psychological factors, or quality of life. Conclusions & Inferences Baseline symptoms were similar in T1DM and T2DM patients, even though T1DM patients had worse gastric emptying delays and higher HbA1c suggesting other factors mediate symptom severity. Symptom scores at 48 weeks decreased in T2DM, but not T1DM patients, despite increased medical and surgical treatment utilization by T1DM patients. Defining causes of different outcomes in diabetic gastroparesis warrants further investigation.
C1 [Koch, K. L.] Wake Forest Univ, Gastroenterol Sect, Winston Salem, NC 27109 USA.
[Hasler, W. L.] Univ Michigan, Div Gastroenterol, Ann Arbor, MI 48109 USA.
[Yates, K. P.; Tonascia, J.; Lee, L.; Miriel, L.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA.
[Parkman, H. P.] Temple Univ, Gastroenterol Sect, Philadelphia, PA 19122 USA.
[Pasricha, P. J.] Johns Hopkins Bayview Med Ctr, Ctr Neurogastroenterol, Baltimore, MD USA.
[Calles-Escandon, J.] MetroHlth Med Ctr, Endocrinol Sect, Cleveland, OH USA.
[Snape, W. J.] Calif Pacific Med Ctr, San Francisco, CA USA.
[Abell, T. L.] Univ Louisville, Digest & Liver Hlth, Louisville, KY 40292 USA.
[Mccallum, R. W.; Sarosiek, I.] Texas Tech Univ, Div Gastroenterol, El Paso, TX USA.
[Nguyen, L. A.] Stanford Univ, Div Gastroenterol, Palo Alto, CA 94304 USA.
[Farrugia, G.] Mayo Clin, Div Gastroenterol & Hepatol, Rochester, MN USA.
[Hamilton, F.] NIDDK, Bethesda, MD 20892 USA.
RP Hasler, WL (reprint author), Univ Michigan Hlth Syst, Taubman Ctr 3912, SPC 5362, Ann Arbor, MI 48109 USA.
EM whasler@umich.edu
FU National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
[U01DK073983, U01DK073975, U01DK073985, U01DK074007, U01DK073974,
U01DK074008]
FX This project received support from the National Institute of Diabetes
and Digestive and Kidney Diseases (NIDDK) (grants U01DK073983,
U01DK073975, U01DK073985, U01DK074007, U01DK073974, U01DK074008) as part
of its funding of the Gastroparesis Clinical Research Consortium.
NR 50
TC 3
Z9 3
U1 1
U2 2
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1350-1925
EI 1365-2982
J9 NEUROGASTROENT MOTIL
JI Neurogastroenterol. Motil.
PD JUL
PY 2016
VL 28
IS 7
BP 1001
EP 1015
DI 10.1111/nmo.12800
PG 15
WC Gastroenterology & Hepatology; Clinical Neurology; Neurosciences
SC Gastroenterology & Hepatology; Neurosciences & Neurology
GA DV9VR
UT WOS:000383290800006
PM 26946489
ER
PT J
AU Costello, RB
Dwyer, JT
Bailey, RL
AF Costello, Rebecca B.
Dwyer, Johanna T.
Bailey, Regan L.
TI Chromium supplements for glycemic control in type 2 diabetes: limited
evidence of effectiveness
SO NUTRITION REVIEWS
LA English
DT Review
DE chromium; fasting plasma glucose; hemoglobin A1c; type 2 diabetes
mellitus
ID PLACEBO-CONTROLLED TRIAL; TOTAL PARENTERAL-NUTRITION; GLUCOSE-TOLERANCE;
DOUBLE-BLIND; INSULIN-RESISTANCE; BREWERS-YEAST; SERUM-LIPIDS; INORGANIC
CHROMIUM; DIETARY CHROMIUM; BODY-COMPOSITION
AB Some adults with type 2 diabetes mellitus (T2DM) believe that chromium-containing supplements will help control their disease, but the evidence is mixed. This narrative review examines the efficacy of chromium supplements for improving glycemic control as measured by decreases in fasting plasma glucose (FPG) or hemoglobin A1c (HbA1c). Using systematic search criteria, 20 randomized controlled trials of chromium supplementation in T2DM patients were identified. Clinically meaningful treatment goals were defined as an FPG of <= 7.2mmol/dL, a decline in HbA1c to <= 7%, or a decrease of >= 0.5% in HbA1c. In only a few randomized controlled trials did FPG (5 of 20), HbA1c (3 of 14), or both (1 of 14) reach the treatment goals with chromium supplementation. HbA1c declined by >= 0.5% in 5 of 14 studies. On the basis of the low strength of existing evidence, chromium supplements have limited effectiveness, and there is little rationale to recommend their use for glycemic control in patients with existing T2DM. Future meta-analyses should include only high-quality studies with similar forms of chromium and comparable inclusion/exclusion criteria to provide scientifically sound recommendations for clinicians.
C1 [Costello, Rebecca B.; Dwyer, Johanna T.; Bailey, Regan L.] NIH, Off Dietary Supplements, 6100 Execut Blvd,Ste 3B01, Bethesda, MD 20892 USA.
[Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Sch Med, Boston, MA 02111 USA.
[Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA.
[Bailey, Regan L.] Purdue Univ, Dept Nutr Sci, W Lafayette, IN 47907 USA.
RP Costello, RB (reprint author), NIH, Off Dietary Supplements, 6100 Execut Blvd,Ste 3B01, Bethesda, MD 20892 USA.
EM costellb@od.nih.gov
FU Office of Dietary Supplements of the National Institutes of Health, US
Department of Health and Human Services; US Department of Agriculture,
Agricultural Research Service [58-1950-0-014]
FX This work was supported by the Office of Dietary Supplements of the
National Institutes of Health, US Department of Health and Human
Services. Partial support was also provided by the US Department of
Agriculture, Agricultural Research Service, under agreement no.
58-1950-0-014.
NR 75
TC 1
Z9 1
U1 10
U2 10
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 0029-6643
EI 1753-4887
J9 NUTR REV
JI Nutr. Rev.
PD JUL
PY 2016
VL 74
IS 7
BP 455
EP 468
DI 10.1093/nutrit/nuw011
PG 14
WC Nutrition & Dietetics
SC Nutrition & Dietetics
GA DV9RO
UT WOS:000383279100004
PM 27261273
ER
PT J
AU Boyles, AL
Yetley, EA
Thayer, KA
Coates, PM
AF Boyles, Abee L.
Yetley, Elizabeth A.
Thayer, Kristina A.
Coates, Paul M.
TI Safe use of high intakes of folic acid: research challenges and paths
forward
SO NUTRITION REVIEWS
LA English
DT Review
DE high vitamin intake; folic acid; research recommendations; safety
ID AUTISM SPECTRUM DISORDERS; NATIONAL-HEALTH; UNITED-STATES; SERUM FOLATE;
SUPPLEMENTATION; POPULATION; RISK
AB Adequate folic acid intake is an effective dietary-based prevention tool for reducing the risk of neural tube defects. Achieving adequate intake for the prevention of neural tube defects frequently requires the consumption of foods fortified with folic acid and/or the use of folic acid-containing dietary supplements. To date, research on the potential for adverse effects of high intakes of folic acid has been limited. Without such research, it is difficult to define a value for high intake. In May 2015, an expert panel was tasked with examining the available scientific literature and making research recommendations within 4 general categories of potential folate-related adverse health effects: cancer, cognition in conjunction with vitamin B-12 deficiency, hypersensitivity-related outcomes, and thyroid and diabetes-related disorders. This article summarizes the expert panel's conclusions, outlines the challenges faced when reviewing the literature, and examines some of the panel's recommendations for research.
C1 [Boyles, Abee L.; Thayer, Kristina A.] NIEHS, Off Hlth Assessment & Translat, Div Natl Toxicol Program, NIH,Dept Hlth & Human Serv, Durham, NC USA.
[Yetley, Elizabeth A.; Coates, Paul M.] NIH, Off Dietary Supplements, Dept Hlth & Human Serv, Bldg 10, Bethesda, MD 20892 USA.
RP Boyles, AL (reprint author), NIEHS, POB 12233,Mail Drop K2-04, Durham, NC 27709 USA.
EM abee.boyles@nih.gov
OI Boyles, Abee/0000-0002-8711-2077
NR 18
TC 2
Z9 2
U1 9
U2 12
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 0029-6643
EI 1753-4887
J9 NUTR REV
JI Nutr. Rev.
PD JUL
PY 2016
VL 74
IS 7
BP 469
EP 474
DI 10.1093/nutrit/nuw015
PG 6
WC Nutrition & Dietetics
SC Nutrition & Dietetics
GA DV9RO
UT WOS:000383279100005
PM 27272334
ER
PT J
AU Huang, SF
Wu, HDI
Chen, YT
Murthy, SRK
Chiu, YT
Chang, Y
Chang, IC
Yang, XY
Loh, YP
AF Huang, Shiu-Feng
Wu, Hong-Dar Isaac
Chen, Ya-Ting
Murthy, Saravana R. K.
Chiu, Yu-Ting
Chang, Yu
Chang, Il-Chi
Yang, Xuyu
Loh, Y. Peng
TI Carboxypeptidase E is a prediction marker for tumor recurrence in
early-stage hepatocellular carcinoma
SO TUMOR BIOLOGY
LA English
DT Article
DE Carboxypeptidase E; Hepatocellular carcinoma; Recurrence-free survival;
Overall survival; Biomarker
ID PATHWAY SORTING RECEPTOR; COLORECTAL-CANCER; POOR-PROGNOSIS; RESECTION;
EXPRESSION; TRANSPLANTATION; ENDOCRINE; SURVIVAL; STRESS; CDNA
AB Tumor recurrence and metastasis are the major causes of death for hepatocellular carcinoma (HCC) patients who are able to receive curative resection. Identifying the predicting biomarkers for tumor recurrence would improve their survival. RNA extracted from fresh frozen tumors and adjacent non-tumor liver tissues of 120 HCC patients were obtained from Taiwan Liver Cancer Network (TLCN) in year 2010 for determination of the carboxypeptidase E (CPE) expression level (including its splicing mutant CPE-Delta N) in the tumor tissue (T) and paired non-tumor liver tissue (N) by real-time quantitative polymerase chain reaction. All patients were male, had chronic hepatitis B virus infection, were in the early pathology stage, and received curative resection. The T/N ratio of the CPE expression level was correlated with the updated survival data from TLCN in 2015. The CPE expression level in the 120 HCC patients was divided into three groups according to the T/N ratio: < 1, aeyen1 and aecurrency sign2, and > 2, respectively. By multivariate analyses, the recurrence-free survival (RFS) was only significantly associated with the pathology stage and the CPE expression level. For overall survival (OS), only the CPE expression level was the significant prognostic factor. The CPE expression level was also significantly correlated with the tumor recurrence for both stage I (p = 0.0106) and stage II patients (p = 0.0006). The CPE mRNA expression level in HCC can be a useful biomarker for predicting tumor recurrence in HCC patients who are in the early pathology stage and able to receive curative resection.
C1 [Huang, Shiu-Feng; Chen, Ya-Ting; Chiu, Yu-Ting; Chang, Yu; Chang, Il-Chi] Natl Hlth Res Inst, Inst Mol & Genom Med, 35 Keyan Rd, Miaoli 350, Taiwan.
[Huang, Shiu-Feng] Chung Shan Med Univ Hosp, Dept Anat Pathol, Taichung, Taiwan.
[Wu, Hong-Dar Isaac] Natl Chung Hsing Univ, Dept Appl Math, Taichung, Taiwan.
[Wu, Hong-Dar Isaac] Natl Chung Hsing Univ, Inst Stat, Taichung, Taiwan.
[Murthy, Saravana R. K.; Yang, Xuyu; Loh, Y. Peng] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Cellular Neurobiol Sect, Program Dev Neurosci, NIH, Bldg 49,Rm 6A-10,49 Convent Dr, Bethesda, MD 20892 USA.
RP Huang, SF (reprint author), Natl Hlth Res Inst, Inst Mol & Genom Med, 35 Keyan Rd, Miaoli 350, Taiwan.; Huang, SF (reprint author), Chung Shan Med Univ Hosp, Dept Anat Pathol, Taichung, Taiwan.; Loh, YP (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Cellular Neurobiol Sect, Program Dev Neurosci, NIH, Bldg 49,Rm 6A-10,49 Convent Dr, Bethesda, MD 20892 USA.
EM sfhuang@nhri.org.tw; lohp@mail.nih.gov
FU National Science Council [NSC-100-c-2320-400-012]; National Health
Research Institutes [MG103-PP-004]; Intramural Research Program of the
Eunice Kennedy Shriver National Institute of Child Health and Human
Development, National Institutes of Health, USA [HD008804]
FX This research was supported by grants from National Science Council
(NSC-100-c-2320-400-012) and National Health Research Institutes
(MG103-PP-004) to Dr. Shiu-Feng Huang and by the Intramural Research
Program of the Eunice Kennedy Shriver National Institute of Child Health
and Human Development, National Institutes of Health, USA (HD008804) to
Dr. Y. Peng Loh.
NR 29
TC 0
Z9 0
U1 0
U2 0
PU SPRINGER
PI DORDRECHT
PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
SN 1010-4283
EI 1423-0380
J9 TUMOR BIOL
JI Tumor Biol.
PD JUL
PY 2016
VL 37
IS 7
BP 9745
EP 9753
DI 10.1007/s13277-016-4814-7
PG 9
WC Oncology
SC Oncology
GA DU4HU
UT WOS:000382174500126
PM 26803519
ER
PT J
AU Gliwa, C
Yurkiewicz, IR
Lehmann, LS
Hull, SC
Jones, N
Berkman, BE
AF Gliwa, Catherine
Yurkiewicz, Ilana R.
Lehmann, Lisa Soleymani
Hull, Sara Chandros
Jones, Nathan
Berkman, Benjamin E.
TI Institutional review board perspectives on obligations to disclose
genetic incidental findings to research participants
SO GENETICS IN MEDICINE
LA English
DT Article
DE genomic sequencing; incidental findings; institutional review board;
research ethics
ID GENOMIC RESEARCH; WORKING GROUP; RECOMMENDATIONS; RETURN; FRAMEWORK;
EXOME
AB Purpose: Researchers' obligations to disclose genetic incidental findings (GIFs) have been widely debated, but there has been little empirical study of the engagement of institutional review boards (IRBs) with this issue.
Methods: This article presents data from the first extensive (n = 796) national survey of IRB professionals' understanding of, experience with, and beliefs surrounding GIFs.
Results: Most respondents had dealt with questions about GIFs (74%), but only a minority (47%) felt prepared to address them. Although a majority believed that there is an obligation to disclose GIFs (78%), there is still not consensus about the supporting ethical principles. Respondents generally did not endorse the idea that researchers' additional time and effort (7%), and lack of resources (29%), were valid reasons for diminishing a putative obligation. Most (96%) supported a right not to know, but this view became less pronounced (63%) when framed in terms of specific case studies.
Conclusions: IRBs are actively engaged with GIFs but have not yet reached consensus. Respondents were uncomfortable with arguments that could be used to limit an obligation to return GIFs. This could indicate that IRBs are providing some of the impetus for the trend toward returning GIFs, although questions remain about the relative contribution of other stakeholders.
C1 [Gliwa, Catherine] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA.
[Gliwa, Catherine; Hull, Sara Chandros; Berkman, Benjamin E.] NIH, Dept Bioeth, Ctr Clin, Bethesda, MD 20892 USA.
[Yurkiewicz, Ilana R.] Harvard Med Sch, Boston, MA USA.
[Lehmann, Lisa Soleymani] Brigham & Womens Hosp, Dept Med, 75 Francis St, Boston, MA 02115 USA.
[Lehmann, Lisa Soleymani] Harvard Med Sch, Dept Global Hlth & Social Med, Boston, MA USA.
[Lehmann, Lisa Soleymani] Harvard TH Chan Sch Publ Hlth, Dept Hlth Policy & Management, Boston, MA USA.
[Hull, Sara Chandros; Berkman, Benjamin E.] NHGRI, Bioeth Core, NIH, Bethesda, MD 20892 USA.
[Jones, Nathan] Univ Wisconsin, Survey Ctr, Madison, WI USA.
RP Berkman, BE (reprint author), NIH, Dept Bioeth, Ctr Clin, Bethesda, MD 20892 USA.; Berkman, BE (reprint author), NHGRI, Bioeth Core, NIH, Bethesda, MD 20892 USA.
EM berkmanbe@mail.nih.gov
OI Lehmann, Lisa Soleymani/0000-0001-8779-1244; Berkman,
Benjamin/0000-0002-9098-0799; Hull, Sara/0000-0001-8742-4997
FU Intramural Research Program of the National Human Genome Research
Institute, National Institutes of Health
FX The authors declare no conflict of interest. This research was supported
in part by the Intramural Research Program of the National Human Genome
Research Institute, National Institutes of Health. The opinions
expressed here are our own and do not reflect the policies or positions
of the National Institutes of Health, the U.S. Public Health Service, or
the U.S. Department of Health and Human Services.
NR 23
TC 1
Z9 1
U1 5
U2 5
PU NATURE PUBLISHING GROUP
PI NEW YORK
PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA
SN 1098-3600
EI 1530-0366
J9 GENET MED
JI Genet. Med.
PD JUL
PY 2016
VL 18
IS 7
BP 705
EP 711
DI 10.1038/gim.2015.149
PG 7
WC Genetics & Heredity
SC Genetics & Heredity
GA DS9XL
UT WOS:000381136700009
PM 26583685
ER
PT J
AU Bharti, SK
Awate, S
Banerjee, T
Brosh, RM
AF Bharti, Sanjay Kumar
Awate, Sanket
Banerjee, Taraswi
Brosh, Robert M., Jr.
TI Getting Ready for the Dance: FANCJ Irons Out DNA Wrinkles
SO GENES
LA English
DT Review
DE G-quadruplex; secondary DNA structure; helicase; replication; genetic
diseases; FANCJ; genomic instability; Fanconi Anemia; cancer
ID G-QUADRUPLEX DNA; WERNER-SYNDROME HELICASE; ANEMIA GROUP J; REV1 PROTEIN
INTERACTS; HOMOLOGOUS RECOMBINATION; REPLICATION FORK; GENOMIC
STABILITY; STRUCTURED DNA; BREAST-CANCER; HUMAN-CELLS
AB Mounting evidence indicates that alternate DNA structures, which deviate from normal double helical DNA, form in vivo and influence cellular processes such as replication and transcription. However, our understanding of how the cellular machinery deals with unusual DNA structures such as G-quadruplexes (G4), triplexes, or hairpins is only beginning to emerge. New advances in the field implicate a direct role of the Fanconi Anemia Group J (FANCJ) helicase, which is linked to a hereditary chromosomal instability disorder and important for cancer suppression, in replication past unusual DNA obstacles. This work sets the stage for significant progress in dissecting the molecular mechanisms whereby replication perturbation by abnormal DNA structures leads to genomic instability. In this review, we focus on FANCJ and its role to enable efficient DNA replication when the fork encounters vastly abundant naturally occurring DNA obstacles, which may have implications for targeting rapidly dividing cancer cells.
C1 [Bharti, Sanjay Kumar; Awate, Sanket; Banerjee, Taraswi; Brosh, Robert M., Jr.] NIA, Lab Mol Gerontol, NIH, Biomed Res Ctr, 251 Bayview Blvd, Baltimore, MD 21224 USA.
RP Brosh, RM (reprint author), NIA, Lab Mol Gerontol, NIH, Biomed Res Ctr, 251 Bayview Blvd, Baltimore, MD 21224 USA.
EM sanjay.bharti@nih.gov; sanket.awate@nih.gov; taraswi.banerjee@nih.gov;
broshr@mail.nih.gov
FU Intramural Research Program of the NIH, National Institute on Aging;
Fanconi Anemia Research Fund
FX This research was supported entirely by the Intramural Research Program
of the NIH, National Institute on Aging and the Fanconi Anemia Research
Fund (RMB). The authors wish to thank Jimmy Burril for the artwork
presented in Figure 1.
NR 101
TC 0
Z9 0
U1 6
U2 6
PU MDPI AG
PI BASEL
PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
SN 2073-4425
J9 GENES-BASEL
JI Genes
PD JUL
PY 2016
VL 7
IS 7
DI 10.3390/genes7070031
PG 14
WC Genetics & Heredity
SC Genetics & Heredity
GA DT5AO
UT WOS:000381493100002
ER
PT J
AU Hammond, JA
Carrington, M
Khakoo, SI
AF Hammond, John A.
Carrington, Mary
Khakoo, Salim I.
TI A vision of KIR variation at super resolution
SO IMMUNOLOGY
LA English
DT Review
DE inhibitory; activating receptors; killer cell immunoglobulin-like
receptors; natural killer cells
ID CELL-RECEPTOR; DIVERSITY; CLONING; NKB1
AB The Ninth Killer Cell Immunoglobulin-Like Receptor (KIR) Workshop was held in Winchester, UK late in the summer of 2015. The extraordinary diversity of KIR and its functional consequences were key themes throughout the meeting. Novel sequencing technologies and new bioinformatics techniques continue to increase our understanding of the genetic diversity and evolution of the KIR; while a deeper understanding of KIR functions, including their specificity for MHC and its peptide ligands, are generating more refined models of their role in disease. Limited to 100 delegates from around the world, this intimate workshop facilitated vigorous discussion, generating new ideas for research in this ever-expanding field.
C1 [Hammond, John A.] Pirbright, Pirbright Inst, Woking, Surrey, England.
[Carrington, Mary] Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Canc & Inflammat Program, Frederick, MD USA.
[Carrington, Mary] Ragon Inst MGH MIT & Harvard, Cambridge, MA USA.
[Khakoo, Salim I.] Univ Southampton, Clin & Expt Sci, Southampton, Hants, England.
RP Khakoo, SI (reprint author), Univ Southampton, Fac Med, Southampton Gen Hosp, Mail Point 811,Level E South Acad Block, Southampton SO16 6YD, Hants, England.
EM S.I.Khakoo@soton.ac.uk
RI Institute, Pirbright/K-4476-2014
FU Frederick National Laboratory for Cancer Research [HHSN261200800001E];
Intramural Research Program of the NIH, Frederick National Laboratory,
Center for Cancer Research; Biotechnology and Biological Sciences
Research Council Institute Strategic Program on Livestock Viral Diseases
awarded
FX We would like to acknowledge the British Society for Immunology, the
meeting sponsors, the participants and those individuals whose work has
not been included in this piece. We thank Peter Parham for critical
reading of the manuscript. This project has been funded in whole or in
part with federal funds from the Frederick National Laboratory for
Cancer Research, under Contract No. HHSN261200800001E. The content of
this publication does not necessarily reflect the views or policies of
the Department of Health and Human Services, nor does mention of trade
names, commercial products or organizations imply endorsement by the
U.S. Government. This Research was supported in part by the Intramural
Research Program of the NIH, Frederick National Laboratory, Center for
Cancer Research. JAH was supported by a Biotechnology and Biological
Sciences Research Council Institute Strategic Program on Livestock Viral
Diseases awarded to The Pirbright Institute.
NR 7
TC 1
Z9 1
U1 2
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0019-2805
EI 1365-2567
J9 IMMUNOLOGY
JI Immunology
PD JUL
PY 2016
VL 148
IS 3
BP 249
EP 252
DI 10.1111/imm.12606
PG 4
WC Immunology
SC Immunology
GA DT2BA
UT WOS:000381284600003
PM 26938992
ER
PT J
AU Hojeij, R
Domingos-Pereira, S
Nkosi, M
Gharbi, D
Derre, L
Schiller, JT
Jichlinski, P
Nardelli-Haefliger, D
AF Hojeij, Rim
Domingos-Pereira, Sonia
Nkosi, Marianne
Gharbi, Dalila
Derre, Laurent
Schiller, John T.
Jichlinski, Patrice
Nardelli-Haefliger, Denise
TI Immunogenic Human Papillomavirus Pseudovirus-Mediated Suicide-Gene
Therapy for Bladder Cancer
SO INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
LA English
DT Article
DE human papillomavirus vectors; tumor-targeting; immunogenic suicide-gene
therapy; bladder cancer
ID INVASIVE UROTHELIAL CARCINOMA; CELL-DEATH; EAU GUIDELINES; COMBINATION;
IMMUNIZATION; VACCINATION; MANAGEMENT; DELIVERY; VECTORS; UPDATE
AB Bladder cancer is the second most common urological malignancy in the world. In 70% of cases it is initially diagnosed as non-muscle-invasive bladder cancer (NMIBC) and it is amenable to local treatments, with intravesical (IVES) Bacillus-Calmette-Guerin (BCG) immunotherapy being routinely used after transurethral resection of the lesion. However, this treatment is associated with significant side-effects and treatment failures, highlighting the necessity of novel strategies. One potent approach is the suicide-gene mediated therapy/prodrug combination, provided tumor-specificity can be ensured and anti-tumor immune responses induced. Using the mouse syngeneic orthotopic MB49-bladder tumor model, here we show that IVES human papillomavirus non-replicative pseudovirions (PsV) can pseudoinfect tumors with a ten-fold higher efficacy than normal bladders. In addition, PsV carrying the suicide-gene herpes-simplex virus thymidine kinase (PsV-TK) combined to Ganciclovir (GCV) led to immunogenic cell-death of tumor cells in vitro and to MB49-specific CD8 T-cells in vivo. This was associated with reduction in bladder-tumor growth and increased mice survival. Altogether, our data show that IVES PsV-TK/GCV may be a promising alternative or combinatory treatment for NMIBC.
C1 [Hojeij, Rim; Domingos-Pereira, Sonia; Nkosi, Marianne; Gharbi, Dalila; Derre, Laurent; Jichlinski, Patrice; Nardelli-Haefliger, Denise] CHU Vaudois, Dept Urol, CH-1011 Lausanne, Switzerland.
[Hojeij, Rim; Domingos-Pereira, Sonia; Nkosi, Marianne; Gharbi, Dalila; Derre, Laurent; Jichlinski, Patrice; Nardelli-Haefliger, Denise] Univ Lausanne, CH-1011 Lausanne, Switzerland.
[Schiller, John T.] NCI, Cellular Oncol Lab, NIH, Bethesda, MD 20892 USA.
RP Nardelli-Haefliger, D (reprint author), CHU Vaudois, Dept Urol, CH-1011 Lausanne, Switzerland.; Nardelli-Haefliger, D (reprint author), Univ Lausanne, CH-1011 Lausanne, Switzerland.
EM reem.hojeij@gmail.com; Sonia.domingos-pereira@chuv.ch;
marianne.nkosi@epfl.ch; dalilagh8@gmail.com; laurent.derre@chuv.ch;
schillej@dc37a.nci.nih.gov; patrice.jichlinski@chuv.ch;
dnardell@hospvd.ch
OI nardelli-haefliger, denise/0000-0003-1812-9905
FU Swiss Cancer League [KFS 2808-08-2011]; Swiss National Science
Foundation [32-153201]
FX This work was supported by the Swiss Cancer League (KFS 2808-08-2011),
the Swiss National Science Foundation (32-153201) and by institutional
funds. We thank Valerie Cesson and Gregory LaSala (Dpt Urology, CHUV,
Lausanne) for their technical help.
NR 42
TC 1
Z9 1
U1 3
U2 3
PU MDPI AG
PI BASEL
PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
SN 1422-0067
J9 INT J MOL SCI
JI Int. J. Mol. Sci.
PD JUL
PY 2016
VL 17
IS 7
AR UNSP 1125
DI 10.3390/ijms17071125
PG 10
WC Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
SC Biochemistry & Molecular Biology; Chemistry
GA DT5DK
UT WOS:000381500900138
ER
PT J
AU Han, Y
Rand, KA
Hazelett, DJ
Ingles, SA
Kittles, RA
Strom, SS
Rybicki, BA
Nemesure, B
Isaacs, WB
Stanford, JL
Zheng, W
Schumacher, FR
Berndt, SI
Wang, ZM
Xu, JF
Rohland, N
Reich, D
Tandon, A
Pasaniuc, B
Allen, A
Quinque, D
Mallick, S
Notani, D
Rosenfeld, MG
Jayani, RS
Kolb, S
Gapstur, SM
Stevens, VL
Pettaway, CA
Yeboah, ED
Tettey, Y
Biritwum, RB
Adjei, AA
Tay, E
Truelove, A
Niwa, S
Chokkalingam, AP
John, EM
Murphy, AB
Signorello, LB
Carpten, J
Leske, MC
Wu, SY
Hennis, AJM
Neslund-Dudas, C
Hsing, AW
Chu, LS
Goodman, PJ
Klein, EA
Zheng, SL
Witte, JS
Casey, G
Lubwama, A
Pooler, LC
Sheng, X
Coetzee, GA
Cook, MB
Chanock, SJ
Stram, DO
Watya, S
Blot, WJ
Conti, DV
Henderson, BE
Haiman, CA
AF Han, Ying
Rand, Kristin A.
Hazelett, Dennis J.
Ingles, Sue A.
Kittles, Rick A.
Strom, Sara S.
Rybicki, Benjamin A.
Nemesure, Barbara
Isaacs, William B.
Stanford, Janet L.
Zheng, Wei
Schumacher, Fredrick R.
Berndt, Sonja I.
Wang, Zhaoming
Xu, Jianfeng
Rohland, Nadin
Reich, David
Tandon, Arti
Pasaniuc, Bogdan
Allen, Alex
Quinque, Dominique
Mallick, Swapan
Notani, Dimple
Rosenfeld, Michael G.
Jayani, Ranveer Singh
Kolb, Suzanne
Gapstur, Susan M.
Stevens, Victoria L.
Pettaway, Curtis A.
Yeboah, Edward D.
Tettey, Yao
Biritwum, Richard B.
Adjei, Andrew A.
Tay, Evelyn
Truelove, Ann
Niwa, Shelley
Chokkalingam, Anand P.
John, Esther M.
Murphy, Adam B.
Signorello, Lisa B.
Carpten, John
Leske, M. Cristina
Wu, Suh-Yuh
Hennis, Anslem J. M.
Neslund-Dudas, Christine
Hsing, Ann W.
Chu, Lisa
Goodman, Phyllis J.
Klein, Eric A.
Zheng, S. Lilly
Witte, John S.
Casey, Graham
Lubwama, Alex
Pooler, Loreall C.
Sheng, Xin
Coetzee, Gerhard A.
Cook, Michael B.
Chanock, Stephen J.
Stram, Daniel O.
Watya, Stephen
Blot, William J.
Conti, David V.
Henderson, Brian E.
Haiman, Christopher A.
TI Prostate Cancer Susceptibility in Men of African Ancestry at 8q24
SO JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
LA English
DT Article
ID GENOME-WIDE ASSOCIATION; LONG NONCODING RNA; RANGE INTERACTION; RISK;
LOCI; JAPANESE; VARIANT; PCAT-1; MYC
AB The 8q24 region harbors multiple risk variants for distinct cancers, including > 8 for prostate cancer. In this study, we conducted fine mapping of the 8q24 risk region (127.8-128.8 Mb) in search of novel associations with common and rare variation in 4853 prostate cancer case patients and 4678 control subjects of African ancestry. All statistical tests were two-sided. We identified three independent associations at P values of less than 5.00 x 10(-8), all of which were replicated in studies from Ghana and Uganda (combined sample = 5869 case patients, 5615 control subjects; rs114798100: risk allele frequency [RAF] = 0.04, per-allele odds ratio [OR] = 2.31, 95% confidence interval [CI] = 2.04 to 2.61, P = 2.38 x 10(-40); rs72725879: RAF = 0.33, OR = 1.37, 95% CI = 1.30 to 1.45, P = 3.04 x 10(-27); and rs111906932: RAF = 0.03, OR = 1.79, 95% CI = 1.53 to 2.08, P = 1.39 x 10(-13)). Risk variants rs114798100 and rs111906923 are only found in men of African ancestry, with rs111906923 representing a novel association signal. The three variants are located within or near a number of prostate cancer-associated long noncoding RNAs (lncRNAs), including PRNCR1, PCAT1, and PCAT2. These findings highlight ancestry-specific risk variation and implicate prostate-specific lncRNAs at the 8q24 prostate cancer susceptibility region.
C1 [Han, Ying; Rand, Kristin A.; Hazelett, Dennis J.; Ingles, Sue A.; Schumacher, Fredrick R.; Casey, Graham; Pooler, Loreall C.; Sheng, Xin; Coetzee, Gerhard A.; Stram, Daniel O.; Conti, David V.; Henderson, Brian E.; Haiman, Christopher A.] Univ Southern Calif, Keck Sch Med, Dept Prevent Med, Los Angeles, CA 90089 USA.
[Coetzee, Gerhard A.] Univ Southern Calif, Keck Sch Med, Dept Urol, Los Angeles, CA 90089 USA.
[Ingles, Sue A.; Schumacher, Fredrick R.; Casey, Graham; Coetzee, Gerhard A.; Stram, Daniel O.; Conti, David V.; Henderson, Brian E.; Haiman, Christopher A.] Univ Southern Calif, Norris Comprehens Canc Ctr, Los Angeles, CA 90089 USA.
[Kittles, Rick A.] Univ Arizona, Coll Med, Tucson, AZ 85724 USA.
[Kittles, Rick A.] Univ Arizona, Ctr Canc, Tucson, AZ 85724 USA.
[Strom, Sara S.] Univ Texas MD Anderson Canc Ctr, Dept Epidemiol, Houston, TX 77030 USA.
[Pettaway, Curtis A.] Univ Texas MD Anderson Canc Ctr, Dept Urol, Houston, TX 77030 USA.
[Rybicki, Benjamin A.] Henry Ford Hosp, Dept Publ Hlth Sci, Detroit, MI 48202 USA.
[Neslund-Dudas, Christine] Henry Ford Hosp, Dept Publ Hlth Sci, Detroit, MI 48202 USA.
[Nemesure, Barbara; Leske, M. Cristina; Wu, Suh-Yuh; Hennis, Anslem J. M.] SUNY Stony Brook, Dept Prevent Med, Stony Brook, NY 11794 USA.
[Isaacs, William B.] Johns Hopkins Hosp & Med Inst, James Buchanan Brady Urol Inst, Baltimore, MD 21205 USA.
[Stanford, Janet L.; Kolb, Suzanne] Fred Hutchinson Canc Res Ctr, Div Publ Hlth Sci, 1124 Columbia St, Seattle, WA 98104 USA.
[Goodman, Phyllis J.] Fred Hutchinson Canc Res Ctr, SWOG Stat Ctr, 1124 Columbia St, Seattle, WA 98104 USA.
[Stanford, Janet L.] Univ Washington, Dept Epidemiol, Sch Publ Hlth, Seattle, WA 98195 USA.
[Zheng, Wei; Blot, William J.] Vanderbilt Univ, Sch Med, Vanderbilt Epidemiol Ctr, Div Epidemiol,Dept Med, Nashville, TN 37212 USA.
[Berndt, Sonja I.; Wang, Zhaoming; Cook, Michael B.; Chanock, Stephen J.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.
[Wang, Zhaoming] SAIC Frederick Inc, NCI DCEG, Canc Genom Res Lab, Frederick, MD 21702 USA.
[Xu, Jianfeng] NorthShore Univ HealthSyst, Program Personalized Canc Care, Evanston, IL 60201 USA.
[Xu, Jianfeng] NorthShore Univ HealthSyst, Dept Surg, Evanston, IL 60201 USA.
[Rohland, Nadin; Reich, David; Tandon, Arti; Allen, Alex; Quinque, Dominique; Mallick, Swapan] Harvard Univ, Harvard Med Sch, Dept Genet, Boston, MA 02115 USA.
[Reich, David; Mallick, Swapan] Harvard Univ, Harvard Med Sch, Howard Hughes Med Inst, Boston, MA 02115 USA.
[Rohland, Nadin; Reich, David; Tandon, Arti; Allen, Alex; Quinque, Dominique; Mallick, Swapan] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA.
[Pasaniuc, Bogdan] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol & Lab Med, Los Angeles, CA 90095 USA.
[Pasaniuc, Bogdan] Univ Calif Los Angeles, David Geffen Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA.
[Notani, Dimple; Rosenfeld, Michael G.; Jayani, Ranveer Singh] Univ Calif San Diego, Sch Med, Dept Med, Howard Hughes Med Inst, San Diego, CA 92103 USA.
[Gapstur, Susan M.; Stevens, Victoria L.] Amer Canc Soc, Epidemiol Res Program, Atlanta, GA 30329 USA.
[Yeboah, Edward D.; Tettey, Yao; Biritwum, Richard B.; Adjei, Andrew A.; Tay, Evelyn] Korle Bu Teaching Hosp, Accra, Ghana.
[Yeboah, Edward D.; Tettey, Yao; Biritwum, Richard B.; Adjei, Andrew A.; Tay, Evelyn] Univ Ghana, Sch Med, Accra, Ghana.
[Tandon, Arti; Niwa, Shelley] Westat Corp, Rockville, MD 20850 USA.
[Chokkalingam, Anand P.] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA.
[John, Esther M.; Hsing, Ann W.; Chu, Lisa] Canc Prevent Inst Calif, Fremont, CA 94538 USA.
[John, Esther M.; Hsing, Ann W.; Chu, Lisa] Stanford Univ, Sch Med, Palo Alto, CA 94304 USA.
[John, Esther M.; Hsing, Ann W.; Chu, Lisa] Stanford Canc Inst, Palo Alto, CA 94304 USA.
[Murphy, Adam B.] Northwestern Univ, Dept Urol, Chicago, IL 60611 USA.
[Signorello, Lisa B.; Blot, William J.] Harvard Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA.
[Carpten, John] Translat Genom Res Inst, Phoenix, AZ 85004 USA.
[Hennis, Anslem J. M.] Univ West Indies, Chron Dis Res Ctr, Bridgetown, Barbados.
[Hennis, Anslem J. M.] Univ West Indies, Fac Med Sci, Bridgetown, Barbados.
[Klein, Eric A.] Cleveland Clin, Glickman Urol & Kidney Inst, Cleveland, OH 44106 USA.
[Zheng, S. Lilly] Wake Forest Sch Med, Ctr Canc Genom, Winston Salem, NC 27157 USA.
[Witte, John S.] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA.
[Witte, John S.] Univ Calif San Francisco, Inst Human Genet, San Francisco, CA 94143 USA.
[Lubwama, Alex; Watya, Stephen] Makerere Univ, Coll Hlth Sci, Sch Publ Hlth, Kampala, Uganda.
[Watya, Stephen] Uro Care, Kampala, Uganda.
RP Haiman, CA (reprint author), Harlyne Norris Res Tower,1450 Biggy St,Room 1504, Los Angeles, CA 90033 USA.
EM haiman@usc.edu
RI Zheng, Wei/O-3351-2013;
OI Zheng, Wei/0000-0003-1226-070X; Jayani, Ranveer
Singh/0000-0002-7646-0711
FU National Institutes of Health (NIH) [CA63464, CA54281, CA1326792,
CA148085, HG004726]; Intramural Research Program of the Division of
Cancer Epidemiology and Genetics, National Cancer Institute (NCI), NIH;
Cancer Research Fund (University of California) [99-00524V-10258,
97-12013, 98-00924V]; Department of Health Services Cancer Research
Program; NCI, NIH, Department of Health and Human Services
[N01-PC-35139]; California Department of Health Services as part of the
statewide cancer reporting program [103885]; Centers for Disease Control
and Prevention [1U58DP0008073]; NIH [CA056678, CA082664, CA092579,
ES011126, S06GM08016, CA092447]; Fred Hutchinson Cancer Research Center;
Intramural Program of the National Human Genome Research Institute;
Public Health Service Cooperative Agreement Grant from the NCI, NIH.
[CA37429, 5UM1CA182883]; Department of Defense (DOD) [W81XWH-07-1-0122];
DOD [DAMD W81XWH-07-1-0203, DAMD W81XWH-06-1-0066, DOD
W81XWH-10-1-0532]; Vanderbilt-Ingram Cancer Center [CA68485]; National
Program of Cancer Registries (NPCR), Centers for Disease Control and
Prevention (CDC); American Cancer Society; Margaret Kersten Ponty
postdoctoral fellowship endowment; Achievement Rewards for College
Scientists (ARCS) Foundation; Los Angeles Founder Chapter; NCI
[CA165862]; [CA68578]; [ES007784]; [DAMD W81XWH-07-1-0645];
[CA140388]; [CA88164]; [CA127298]
FX The Multiethnic Cohort Study (MEC) and the genotyping in this study were
supported by National Institutes of Health (NIH) grants CA63464,
CA54281, CA1326792, CA148085, and HG004726. Genotyping of the Prostate,
Lung, Colorectal, and Ovarian Cancer Screening Trial (PLCO) samples was
funded by the Intramural Research Program of the Division of Cancer
Epidemiology and Genetics, National Cancer Institute (NCI), NIH. The Los
Angeles Study of Aggressive Prostate Cancer (LAAPC) was funded by grant
99-00524V-10258 from the Cancer Research Fund, under Interagency
Agreement #97-12013 (University of California contract #98-00924V) with
the Department of Health Services Cancer Research Program. Cancer
incidence data for the MEC and LAAPC studies have been collected by the
Los Angeles Cancer Surveillance Program of the University of Southern
California with federal funds from the NCI, NIH, Department of Health
and Human Services (under Contract No. N01-PC-35139), and the California
Department of Health Services as part of the statewide cancer reporting
program mandated by California Health and Safety Code Section 103885,
and grant number 1U58DP0008073 from the Centers for Disease Control and
Prevention. The King County (Washington) Prostate Cancer Study (KCPCS)
was supported by NIH grants CA056678, CA082664, and CA092579, with
additional support from the Fred Hutchinson Cancer Research Center and
the Intramural Program of the National Human Genome Research Institute.
The Prostate Cancer Case-Control Studies at MD Anderson (MDA) was
supported by grants CA68578, ES007784, DAMD W81XWH-07-1-0645, and
CA140388. The Prostate Cancer Genetics Study (CaP Genes) was supported
by CA88164 and CA127298. The Selenium and Vitamin E Cancer Prevention
Trial (SELECT) was funded by Public Health Service Cooperative Agreement
Grant CA37429 and 5UM1CA182883 from the NCI, NIH. The Gene-Environment
in Prostate Cancer Study (GECAP) was supported by NIH grant ES011126.
The Identifying Prostate Cancer Genes study (IPCG) was supported by the
Department of Defense (DOD) grant W81XWH-07-1-0122. The Case-Control
Study of Prostate Cancer among African Americans in Washington, DC
(DCPC) was supported by NIH grant S06GM08016 and DOD grants DAMD
W81XWH-07-1-0203, DAMD W81XWH-06-1-0066, and DOD W81XWH-10-1-0532. The
Southern Community Cohort Study (SCCS) was funded by NIH grant CA092447.
SCCS sample preparation was conducted at the Epidemiology Biospecimen
Core Lab, which is supported in part by the Vanderbilt-Ingram Cancer
Center (CA68485). Data on SCCS cancer case patients used in this
publication were provided by the Alabama Statewide Cancer Registry;
Kentucky Cancer Registry; Tennessee Department of Health, Office of
Cancer Surveillance; Florida Cancer Data System; North Carolina Central
Cancer Registry, North Carolina Division of Public Health; Georgia
Comprehensive Cancer Registry; Louisiana Tumor Registry; Mississippi
Cancer Registry; South Carolina Central Cancer Registry; Virginia
Department of Health, Virginia Cancer Registry; Arkansas Department of
Health, Cancer Registry. The Arkansas Central Cancer Registry was fully
funded by a grant from the National Program of Cancer Registries (NPCR),
Centers for Disease Control and Prevention (CDC). Data on SCCS cancer
case patients from Mississippi were collected by the Mississippi Cancer
Registry, which participates in the NPCR of the CDC. The contents of
this publication are solely the responsibility of the authors and do not
necessarily represent the official views of the CDC or the Mississippi
Cancer Registry.; The Cancer Prevention Study II Nutrition Cohort
(CPC-II) was supported by the American Cancer Society. KAR was supported
in part by the Margaret Kersten Ponty postdoctoral fellowship endowment,
Achievement Rewards for College Scientists (ARCS) Foundation, Los
Angeles Founder Chapter. Sequencing in this study was supported by NCI
grant CA165862.
NR 18
TC 4
Z9 4
U1 2
U2 2
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 0027-8874
EI 1460-2105
J9 JNCI-J NATL CANCER I
JI JNCI-J. Natl. Cancer Inst.
PD JUL
PY 2016
VL 108
IS 7
AR djv431
DI 10.1093/jnci/djv431
PG 5
WC Oncology
SC Oncology
GA DU9FT
UT WOS:000382522500017
ER
PT J
AU Sabbatino, F
Wang, YY
Scognamiglio, G
Favoino, E
Feldman, SA
Villani, V
Flaherty, KT
Nota, S
Giannarelli, D
Simeone, E
Anniciello, AM
Palmieri, G
Pepe, S
Botti, G
Ascierto, PA
Ferrone, CR
Ferrone, S
AF Sabbatino, Francesco
Wang, Yangyang
Scognamiglio, Giosue
Favoino, Elvira
Feldman, Steven A.
Villani, Vincenzo
Flaherty, Keith T.
Nota, Sjoerd
Giannarelli, Diana
Simeone, Ester
Anniciello, Anna M.
Palmieri, Giuseppe
Pepe, Stefano
Botti, Gerardo
Ascierto, Paolo A.
Ferrone, Cristina R.
Ferrone, Soldano
TI Antitumor Activity of BRAF Inhibitor and IFN alpha Combination in
BRAF-Mutant Melanoma
SO JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
LA English
DT Article
ID CELLS-IN-VITRO; INTERFERON-ALPHA; METASTATIC MELANOMA; HUMAN-LEUKOCYTE;
PRIMARY TUMORS; UP-REGULATION; OPEN-LABEL; EXPRESSION; ANTIGEN; RECEPTOR
AB Background: BRAF(V600E)-mediated MAPK pathway activation is associated in melanoma cells with IFNAR1 downregulation. IFNAR1 regulates melanoma cell sensitivity to IFN alpha, a cytokine used for the adjuvant treatment of melanoma. These findings and the limited therapeutic efficacy of BRAF-I prompted us to examine whether the efficacy of IFN alpha therapy of BRAF(V600E) melanoma can be increased by its combination with BRAF-I.
Methods: BRAF/NRAS genotype, ERK activation, IFNAR1, and HLA class I expression were tested in 60 primary melanoma tumors from treatment-naive patients. The effect of BRAF-I on IFNAR1 expression was assessed in three melanoma cell lines and in four biopsies of BRAF(V600E) metastases. The antiproliferative, pro-apoptotic and immunomodulatory activity of BRAF-I and IFN alpha combination was tested in vitro and in vivo utilizing three melanoma cell lines, HLA class I-MA peptide complex-specific T-cells and immunodeficient mice (5 per group for survival and 10 per group for tumor growth inhibition). All statistical tests were two-sided. Differences were considered statistically significant when the P value was less than .05.
Results: The IFNAR1 level was statistically significantly (P < .001) lower in BRAF(V600E) primary melanoma tumors than in BRAF wild-type tumors. IFNAR1 downregulation was reversed by BRAF-I treatment in the three melanoma cell lines (P <= .02) and in three out of four metastases. The IFNAR1 level in the melanoma tumors analyzed was increased as early as 10 to 14 days following the beginning of the treatment. These changes were associated with: 1) an increased susceptibility in vitro of melanoma cells to the antiproliferative (P <= .04), pro-apoptotic (P <= .009) and immunomodulatory activity, including upregulation of HLA class I antigen APM component (P <= .04) and MA expression as well as recognition by cognate T-cells (P < .001), of BRAF-I and IFN alpha combination and 2) an increased survival (P < .001) and inhibition of tumor growth of melanoma cells (P < .001) in vivo by BRAF-I and IFN alpha combination.
Conclusions: The described results provide a strong rationale for the clinical trials implemented in BRAFV600E melanoma patients with BRAF-I and IFN alpha combination.
C1 [Sabbatino, Francesco; Wang, Yangyang; Favoino, Elvira; Villani, Vincenzo; Ferrone, Cristina R.; Ferrone, Soldano] Harvard Med Sch, Massachusetts Gen Hosp, Dept Surg, 55 Fruit St, Boston, MA 02114 USA.
[Flaherty, Keith T.] Harvard Med Sch, Massachusetts Gen Hosp, Dept Med Oncol, Boston, MA 02114 USA.
[Nota, Sjoerd; Ferrone, Soldano] Harvard Med Sch, Massachusetts Gen Hosp, Dept Orthopaed Surg, Boston, MA 02114 USA.
[Scognamiglio, Giosue; Anniciello, Anna M.; Botti, Gerardo] Fdn G Pascale, Ist Nazl Tumori, Unit Pathol, Naples, Italy.
[Simeone, Ester; Ascierto, Paolo A.] Fdn G Pascale, Ist Nazl Tumori, Unit Melanoma Canc Immunotherapy & Innovat Therap, Naples, Italy.
[Feldman, Steven A.] NCI, Surg Branch, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Giannarelli, Diana] Regina Elena Inst Canc Res, Biostat Unit, Rome, Italy.
[Palmieri, Giuseppe] CNR, Inst Biomol Chem, Unit Canc Genet, Sassari, Italy.
[Sabbatino, Francesco; Pepe, Stefano] Univ Salerno, Dept Med & Surg, Salerno, Italy.
[Favoino, Elvira] IRCCS Saverio de Bellis, Natl Inst Digest Dis, Lab Cellular & Mol Biol, Castellana Grotte, BA, Italy.
RP Ferrone, S (reprint author), Harvard Med Sch, Massachusetts Gen Hosp, Dept Surg, 55 Fruit St, Boston, MA 02114 USA.
EM sferrone@mgh.harvard.edu
OI Palmieri, Giuseppe/0000-0002-4350-2276
FU National Cancer Institute (PHS grants) [RO1CA138188, P50CA121973];
Fondazione Umberto Veronesi; Susan G. Komen for the Cure Foundation
[KG111486]; Centro per la Comunicazione e la Ricerca of the Collegio
Ghislieri of Pavia
FX This study was supported by the National Cancer Institute (PHS grants
RO1CA138188 and P50CA121973 to SF), by Fondazione Umberto Veronesi
(Fondazione Umberto Veronesi Post Doctoral Fellowship by FS), by the
Susan G. Komen for the Cure Foundation (Susan Komen Post Doctoral
Fellowship KG111486 by YW), and by Centro per la Comunicazione e la
Ricerca of the Collegio Ghislieri of Pavia (Research Fellowship by VV).
NR 38
TC 3
Z9 3
U1 5
U2 5
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 0027-8874
EI 1460-2105
J9 JNCI-J NATL CANCER I
JI JNCI-J. Natl. Cancer Inst.
PD JUL
PY 2016
VL 108
IS 7
AR djv435
DI 10.1093/jnci/djv435
PG 11
WC Oncology
SC Oncology
GA DU9FT
UT WOS:000382522500008
ER
PT J
AU Villalona-Calero, MA
Duan, WR
Zhao, WQ
Shilo, K
Schaaf, LJ
Thurmond, J
Westman, JA
Marshall, J
Li, XB
Ji, JP
Rose, J
Lustberg, M
Bekaii-Saab, T
Chen, A
Timmers, C
AF Villalona-Calero, Miguel A.
Duan, Wenrui
Zhao, Weiqiang
Shilo, Konstantin
Schaaf, Larry J.
Thurmond, Jennifer
Westman, Judith A.
Marshall, John
Li Xiaobai
Ji, Jiuping
Rose, Jeffrey
Lustberg, Maryam
Bekaii-Saab, Tanios
Chen, Alice
Timmers, Cynthia
TI Veliparib Alone or in Combination with Mitomycin C in Patients with
Solid Tumors With Functional Deficiency in Homologous Recombination
Repair
SO JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
LA English
DT Article
ID FANCONI-ANEMIA SUBTYPE; POLY(ADP-RIBOSE) POLYMERASE; DNA-REPAIR;
OVARIAN-CANCER; BIALLELIC MUTATIONS; SOMATIC MUTATIONS; BRCA1
DEFICIENCY; LUNG-CANCER; PATHWAY; RESISTANCE
AB Background: BRCA germline mutations are being targeted for development of PARP inhibitors. BRCA genes collaborate with several others in the Fanconi Anemia (FA) pathway. We screened cancer patients' tumors for FA functional defects then aimed to establish the safety/feasibility of administering PARP inhibitors as monotherapy and combined with a DNA-breaking agent.
Methods: Patients underwent FA functional screening for the presence (or lack) of tumor FancD2 nuclear foci formation on their archival tumor material, utilizing a newly developed method (Fanconi Anemia triple-stain immunofluorescence [FATSI]), performed in a Clinical Laboratory Improvement Amendments-certified laboratory. FATSI-negative patients were selected for enrollment in a two-arm dose escalation trial of veliparib, or veliparib/mitomycin-C (MMC).
Results: One hundred eighty-five of 643 (28.7%) screened patients were FATSI-negative. Sixty-one received veliparib or veliparib/MMC through 14 dose levels. Moderate/severe toxicities included fatigue (DLT at veliparib 400 mg BID), diarrhea, and thrombocytopenia. Recommended doses are 300 mg BID veliparib and veliparib 200 mg BID for 21 days following 10 mg/m(2) MMC every 28 days. Six antitumor responses occurred, five in the combination arm (3 breast, 1 ovarian, 1 endometrial [uterine], and 1 non-small cell lung cancer). Two patients have received 36 and 60 cycles to date. BRCA germline analysis among 51 patients revealed five deleterious mutations while a targeted FA sequencing gene panel showed missense/nonsense mutations in 29 of 49 FATSI-negative tumor specimens.
Conclusions: FATSI screening showed that a substantial number of patients' tumors have FA functional deficiency, which led to germline alterations in several patients' tumors. Veliparib alone or with MMC was safely administered to these patients and produced clinical benefit in some. However, a better understanding of resistance mechanisms in this setting is needed.
C1 [Villalona-Calero, Miguel A.; Duan, Wenrui; Rose, Jeffrey; Lustberg, Maryam; Bekaii-Saab, Tanios] Ohio State Univ, Div Med Oncol, Columbus, OH 43210 USA.
[Westman, Judith A.] Ohio State Univ, Clin Canc Genet, Columbus, OH 43210 USA.
[Zhao, Weiqiang; Shilo, Konstantin] Ohio State Univ, Dept Pathol, Columbus, OH 43210 USA.
[Villalona-Calero, Miguel A.; Duan, Wenrui; Schaaf, Larry J.; Thurmond, Jennifer; Bekaii-Saab, Tanios; Timmers, Cynthia] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA.
[Li Xiaobai] Ohio State Univ, Ctr Biostat, Columbus, OH 43210 USA.
[Marshall, John] Georgetown Univ, Lombardi Canc Ctr, Washington, DC 20007 USA.
[Ji, Jiuping; Chen, Alice] NCI, Bethesda, MD 20892 USA.
RP Villalona-Calero, MA (reprint author), Miami Canc Inst, 1575 San Ignacio Ave,Suite 100, Coral Gables, FL 33146 USA.
EM miguelvil@BaptistHealth.net
OI S, K/0000-0002-6702-3130
FU National Institutes of Health (NIH) [R01-CA152101,
N01-CM-2011-00070(HHSN261201100070C), OSUCCC P30 CA016058-38]
FX This work was supported by National Institutes of Health (NIH)
grantsR01-CA152101, N01-CM-2011-00070(HHSN261201100070C) to MAV, and
OSUCCC P30 CA016058-38.
NR 58
TC 1
Z9 1
U1 0
U2 0
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 0027-8874
EI 1460-2105
J9 JNCI-J NATL CANCER I
JI JNCI-J. Natl. Cancer Inst.
PD JUL
PY 2016
VL 108
IS 7
AR djv437
DI 10.1093/jnci/djv437
PG 10
WC Oncology
SC Oncology
GA DU9FT
UT WOS:000382522500011
ER
PT J
AU Tisnado, D
Malin, J
Kahn, K
Landrum, MB
Fletcher, R
Klabunde, C
Clauser, S
Rogers, SO
Keating, NL
AF Tisnado, Diana
Malin, Jennifer
Kahn, Katherine
Landrum, Mary Beth
Fletcher, Robert
Klabunde, Carrie
Clauser, Steven
Rogers, Selwyn O., Jr.
Keating, Nancy L.
TI Variations in Oncologist Recommendations for Chemotherapy for Stage IV
Lung Cancer: What Is the Role of Performance Status?
SO JOURNAL OF ONCOLOGY PRACTICE
LA English
DT Article
ID EARLY PALLIATIVE CARE; SUPPORTIVE CARE; THERAPEUTIC APPROACH; CLINICAL
ONCOLOGY; AMERICAN SOCIETY; OF-LIFE; OUTCOMES; QUALITY; TRIALS;
METAANALYSIS
AB Purpose:Chemotherapy prolongs survival in patients with advanced non-small-cell lung cancer. However, few studies have included patients with poor performance status. This study examined rates of oncologists' recommendations for chemotherapy by patient performance status and symptoms and how physician characteristics influence chemotherapy recommendations.Methods:We surveyed medical oncologists involved in the care of a population-based cohort of patients with lung cancer from the CanCORS (Cancer Care Outcomes Research and Surveillance) study. Physicians were queried about their likelihood to recommend chemotherapy to patients with stage IV lung cancer with varying performance status (Eastern Cooperative Oncology Group performance status 0 [good] v 3 [poor]) and presence or absence of tumor-related pain. Repeated measures logistic regression was used to estimate the independent associations of patients' performance status and symptoms and physicians' demographic and practice characteristics with chemotherapy recommendations.Results:Nearly all physicians (adjusted rate, 97% to 99%) recommended chemotherapy for patients with good performance status, and approximately half (adjusted rate, 38% to 53%) recommended chemotherapy for patients with poor performance status (P < .001). Compared with patient factors, physician and practice characteristics were less strongly associated with chemotherapy recommendations in adjusted analyses.Conclusion:Strong consensus among oncologists exists for chemotherapy in patients with advanced non-small-cell lung cancer and good performance status. However, the relatively high rate of chemotherapy recommendations for patients with poor performance status despite the unfavorable risk-benefit profile highlights the need for ongoing work to define high-value care in oncology and to implement and evaluate strategies to align incentives for such care.
C1 [Tisnado, Diana] Calif State Univ Fullerton, 800 N State Coll Blvd,Rm KHS 142, Fullerton, CA 92834 USA.
Anthem, Woodland Hills, CA USA.
Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA.
RAND Corp, Santa Monica, CA USA.
Harvard Med Sch, Boston, MA USA.
Brigham & Womens Hosp, 75 Francis St, Boston, MA 02115 USA.
NCI, Bethesda, MD 20892 USA.
Patient Ctr Outcomes Res Inst, Washington, DC USA.
Univ Texas Med Branch Hlth, Galveston, TX USA.
RP Tisnado, D (reprint author), Calif State Univ Fullerton, 800 N State Coll Blvd,Rm KHS 142, Fullerton, CA 92834 USA.
EM dtisnado@fullerton.edu
FU NCI NIH HHS [K24 CA181510, U01 CA093324, U01 CA093326, U01 CA093329, U01
CA093332, U01 CA093339, U01 CA093344, U01 CA093348]; NIA NIH HHS [P30
AG021684]
NR 40
TC 0
Z9 0
U1 1
U2 1
PU AMER SOC CLINICAL ONCOLOGY
PI ALEXANDRIA
PA 2318 MILL ROAD, STE 800, ALEXANDRIA, VA 22314 USA
SN 1554-7477
EI 1935-469X
J9 J ONCOL PRACT
JI J. Oncol. Pract.
PD JUL
PY 2016
VL 12
IS 7
BP 651
EP +
DI 10.1200/JOP.2015.008425
PG 14
WC Health Care Sciences & Services
SC Health Care Sciences & Services
GA DT3JL
UT WOS:000381376900015
PM 27271507
ER
PT J
AU Fragoso, CAV
McAvay, G
Van Ness, PH
Metter, EJ
Ferrucci, L
Yaggi, HK
Concato, J
Gill, TM
AF Fragoso, Carlos A. Vaz
McAvay, Gail
Van Ness, Peter H.
Metter, E. Jeffrey
Ferrucci, Luigi
Yaggi, H. Klar
Concato, John
Gill, Thomas M.
TI Aging-Related Considerations When Evaluating the Forced Expiratory
Volume in 1 Second (FEV1) Over Time
SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL
SCIENCES
LA English
DT Article
DE Aging; Spirometry; Lung diseases; Cardiovascular diseases; Epidemiology
ID LUNG-FUNCTION; RESPIRATORY IMPAIRMENT; NATURAL-HISTORY; OLDER PERSONS;
SPIROMETRY; DISEASE; PREVALENCE; VALUES
AB Background: Forced expiratory volume in 1 second (FEV1) over time is commonly expressed in liters and percent predicted (%Pred), or alternatively in L/m(3) and Z-scores. which approach is more clinically meaningful has not been evaluated. Because it uniquely accounts for the effect of aging on FEV1 and spirometric performance, we hypothesized that the Z-score approach is more clinically meaningful, based on associations between cardiopulmonary predictors and FEV1 over time.
Methods: Using linear mixed-effects models and data from the Baltimore Longitudinal Study on Aging, including 501 white participants aged 40-95 who had completed at least three longitudinal spirometric assessments, we evaluated the associations between cardiopulmonary predictors (obesity, smoking status, hypertension, chronic bronchitis, diabetes mellitus, and myocardial infarction) and FEV1 over time, in liters, %Pred, L/m(3), and Z-scores.
Results: Mean baseline values for FEV1 were 3.240 L, 96.4% Pred, 0.621 L/m(3), and -0.239 as a Z-score (40.6th percentile). The annual decline in FEV1 was 0.040 L, 0.234 %Pred, 0.007 L/m(3), and 0.008 Z-score units. Baseline age was associated with FEV1 over time in liters and L/m(3) (p < .001), and included a time interaction for %Pred (p < .001), but was not associated with Z-scores (p = .933). The associations of cardiopulmonary predictors with FEV1 over time were all significant when using Z-scores (p < <.05.05), but varied for other methods of expressing FEV1.
Conclusion: A Z-score approach is more clinically meaningful when evaluating FEV1 over time, as it accounted for the effect of aging and was more frequently associated with multiple cardiopulmonary predictors.
C1 [Fragoso, Carlos A. Vaz; Yaggi, H. Klar; Concato, John] Vet Affairs Clin Epidemiol Res Ctr, West Haven, CT USA.
[Fragoso, Carlos A. Vaz; McAvay, Gail; Van Ness, Peter H.; Yaggi, H. Klar; Concato, John; Gill, Thomas M.] Yale Univ, Sch Med, Dept Internal Med, New Haven, CT 06510 USA.
[Metter, E. Jeffrey] Univ Tennessee, Ctr Hlth Sci, Dept Neurol, Memphis, TN 38163 USA.
[Ferrucci, Luigi] NIA, Harbor Hosp, Baltimore, MD 21224 USA.
RP Fragoso, CAV (reprint author), VA Connecticut Healthcare Syst, 950 Campbell Ave, West Haven, CT 06516 USA.
EM carlos.fragoso@yale.edu
FU Yale Claude D. Pepper Older Americans Independence Center [P30AG021342];
VA Merit Award; VA Cooperative Studies Program; NIA Academic Leadership
Award [K07AG043587]
FX The analytical phase of this study was conducted at the Yale Claude D.
Pepper Older Americans Independence Center (P30AG021342). C.A.V.F. is
supported by a VA Merit Award. J.C. is supported by the VA Cooperative
Studies Program. T.M.G is the recipient of an NIA Academic Leadership
Award (K07AG043587).
NR 30
TC 2
Z9 2
U1 2
U2 2
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1079-5006
EI 1758-535X
J9 J GERONTOL A-BIOL
JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci.
PD JUL
PY 2016
VL 71
IS 7
BP 929
EP 934
DI 10.1093/gerona/glv201
PG 6
WC Geriatrics & Gerontology; Gerontology
SC Geriatrics & Gerontology
GA DT0YW
UT WOS:000381209900012
ER
PT J
AU Schrack, JA
Zipunnikov, V
Simonsick, EM
Studenski, S
Ferrucci, L
AF Schrack, Jennifer A.
Zipunnikov, Vadim
Simonsick, Eleanor M.
Studenski, Stephanie
Ferrucci, Luigi
TI Rising Energetic Cost of Walking Predicts Gait Speed Decline With Aging
SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL
SCIENCES
LA English
DT Article
DE Functional performance; Gait; Metabolism; Physical function; Physiology
ID LOWER-EXTREMITY FUNCTION; RESTING METABOLIC-RATE; OLDER-ADULTS;
SUBSEQUENT DISABILITY; WOMEN; AGE; EXPENDITURE; TREADMILL; HEALTH; PACE
AB Background: Slow gait is a robust biomarker of health and a predictor of functional decline and death in older adults, yet factors contributing to the decline in gait speed with aging are not well understood. Previous research suggests that the energetic cost of walking at preferred speed is inversely associated with gait speed, but whether individuals with a rising energetic cost of walking experience a steeper rate of gait speed decline has not been investigated.
Methods: In participants of the Baltimore Longitudinal Study of Aging, the energetic cost of overground walking at preferred speed (mL/kg/m) was assessed between 2007 and 2014 using a portable indirect calorimeter. The longitudinal association between the energetic cost of walking and usual gait speed over 6 meters (m/s) was assessed with multivariate linear regression models, and the risk of slow gait (<1.0 m/s) was analyzed using Cox proportional hazards models.
Results: The study population consisted of 457 participants aged 40 and older who contributed 1,121 person-visits to the analysis. In fully adjusted models, increases in the energetic cost of walking predicted the rate of gait speed decline in those older than 65 years (beta = -0.008 m/s, p < .001). Moreover, those with a higher energetic cost of walking (>0.17 mL/kg/m) had a 57% greater risk of developing slow gait compared with a normal energetic cost of walking (<= 0.17 mL/kg/m; adjusted hazard ratio = 1.57, 95% confidence interval: 1.01-2.46).
Conclusions: These findings suggest that strategies to maintain walking efficiency hold significant implications for maintaining mobility in late life. Efforts to curb threats to walking efficiency should focus on therapies to treat gait and balance impairments, and reduce clinical disease burden.
C1 [Schrack, Jennifer A.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, 615 North Wolfe St,E7144, Baltimore, MD 21205 USA.
[Schrack, Jennifer A.; Simonsick, Eleanor M.; Studenski, Stephanie; Ferrucci, Luigi] NIA, Longitudinal Studies Sect, Translat Gerontol Branch, NIH, Baltimore, MD 21224 USA.
[Zipunnikov, Vadim] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Biostat, Baltimore, MD USA.
RP Schrack, JA (reprint author), Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, 615 North Wolfe St,E7144, Baltimore, MD 21205 USA.
EM jschrac1@jhu.edu
FU Intramural Research Program of the NIH, National Institute on Aging;
[K01AG048765]; [HHSN311210300177P]
FX This research was supported by the Intramural Research Program of the
NIH, National Institute on Aging. J.A.S was supported by K01AG048765 and
HHSN311210300177P.
NR 36
TC 2
Z9 2
U1 5
U2 5
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1079-5006
EI 1758-535X
J9 J GERONTOL A-BIOL
JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci.
PD JUL
PY 2016
VL 71
IS 7
BP 947
EP 953
DI 10.1093/gerona/glw002
PG 7
WC Geriatrics & Gerontology; Gerontology
SC Geriatrics & Gerontology
GA DT0YW
UT WOS:000381209900015
PM 26850913
ER
PT J
AU Simonsick, EM
Chia, CW
Mammen, JS
Egan, JM
Ferrucci, L
AF Simonsick, Eleanor M.
Chia, Chee W.
Mammen, Jennifer S.
Egan, Josephine M.
Ferrucci, Luigi
TI Free Thyroxine and Functional Mobility, Fitness, and Fatigue in
Euthyroid Older Men and Women in the Baltimore Longitudinal Study of
Aging
SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL
SCIENCES
LA English
DT Article
DE Free thyroxine; Physical function; Aging; Fitness; Fatigue
ID THYROID-FUNCTION; SUBCLINICAL HYPOTHYROIDISM; COGNITIVE FUNCTION;
PHYSICAL FUNCTION; METABOLIC-RATE; ADULTS; AGE; HEALTH; POPULATION;
MORTALITY
AB Background: Emerging evidence suggests that mildly down-regulated thyroid function in older persons may protect and/or reflect maintained health.
Methods: Using observational data collected between January 2006 and March 2014 on a volunteer sample of 602 men and women aged 68-97 years with normal thyroid function participating in the Baltimore Longitudinal Study of Aging, this study examines the concurrent relationship between reported walking ability, usual and rapid gait speed, endurance walk performance, fatigability, and reported energy level with respect to free thyroxine (FT4) within the normal range (0.76-1.50 ng/dL) as a continuous variable and categorized as low (lower quartile), medium (interquartile), or high (upper quartile).
Results: Adjusting for sex, age, race, height, weight, exercise and smoking, reported walking ability, usual and rapid gait speed, 400-m time, fatigability, and reported energy level were less favorable with increasing FT4 (p =.013 to <. 001). In sex-strata, similar associations were observed except for walking ability in men and energy level in women. Categorical analyses revealed that persons with low FT4 exhibited better functional mobility, fitness, and reported energy than persons with intermediate or high levels (p < .05 for all). Persons with high-normal versus medium FT4 had slower usual and rapid gait speed (p < .05) only.
Conclusion: Older adults with low-normal FT4 exhibit better mobility, fitness, and fatigue profiles. Mildly down-regulated thyroid function appears to align with better function in old age and may serve as a biomarker of healthy longevity.
C1 [Simonsick, Eleanor M.; Chia, Chee W.; Egan, Josephine M.; Ferrucci, Luigi] NIA, Intramural Res Program, Baltimore, MD 21224 USA.
[Simonsick, Eleanor M.; Chia, Chee W.; Mammen, Jennifer S.; Egan, Josephine M.; Ferrucci, Luigi] Johns Hopkins Univ, Sch Med, Baltimore, MD USA.
RP Simonsick, EM (reprint author), 3001 S Hanover St,5th Floor, Baltimore, MD 21224 USA.
EM simonsickel@mail.nih.gov
FU Intramural Research Program of the National Institute on Aging, National
Institutes of Health
FX This research was supported by the Intramural Research Program of the
National Institute on Aging, National Institutes of Health.
NR 33
TC 4
Z9 4
U1 0
U2 0
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1079-5006
EI 1758-535X
J9 J GERONTOL A-BIOL
JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci.
PD JUL
PY 2016
VL 71
IS 7
BP 961
EP 967
DI 10.1093/gerona/glv226
PG 7
WC Geriatrics & Gerontology; Gerontology
SC Geriatrics & Gerontology
GA DT0YW
UT WOS:000381209900017
PM 26791089
ER
PT J
AU Rosario, BL
Rosso, AL
Aizenstein, HJ
Harris, T
Newman, AB
Satterfield, S
Studenski, SA
Yaffe, K
Rosano, C
AF Rosario, Bedda L.
Rosso, Andrea L.
Aizenstein, Howard J.
Harris, Tamara
Newman, Anne B.
Satterfield, Suzanne
Studenski, Stephanie A.
Yaffe, Kristine
Rosano, Caterina
CA Hlth ABC Study
TI Cerebral White Matter and Slow Gait: Contribution of Hyperintensities
and Normal-appearing Parenchyma
SO JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL
SCIENCES
LA English
DT Article
DE White matter hyperintensities; Fractional anisotropy; Normal-appearing
white matter; DTI; Gait speed
ID OLDER-PEOPLE; FALL RISK; ADULTS; BRAIN; SPEED; DISORDERS; INTEGRITY
AB Background. White matter hyperintensities (WMH), a common marker of cerebral small vessel disease, and lower microstructural integrity of normal-appearing white matter are associated with slower gait. How these cerebral measures interact in relation to slower gait is unknown. We assessed whether microstructural integrity of normal-appearing white matter, measured by fractional anisotropy (FA), moderates the association of higher WMH with slower gait.
Methods. WMH, FA, and gait speed were acquired for 265 community-dwelling older adults (average age = 82.9 years).
Results. The inverse association between WMH and gait was robust to adjustment for age, gender, muscle strength, obesity, stroke, and hypertension (fully adjusted model: beta(s) = -0.19, p = .001). The interaction between WMH and FA was significant; analyses stratified by FA showed that the inverse association between WMH and gait speed was significant only for those with low FA (FA < median, fully adjusted model: beta(s) = -0.28, p = .001). Voxel-based results were similar for participants with FA less than median, there was an inverse association between gait speed and WMH which extended throughout the white matter (genu and body of corpus callosum, anterior limb of internal capsule, corona radiata, and superior longitudinal and fronto-occipital fasciculus). In contrast, for participants with FA = median, the association was limited to the genu of corpus callosum, the cingulum, and the inferior longitudinal fasciculus.
Conclusions. Microstructural integrity is a moderating factor in the association between WMH and gait. Future studies should examine whether higher microstructural integrity represents a source of compensation in those with greater WMH burden to maintain function in late life.
C1 [Rosario, Bedda L.; Rosso, Andrea L.; Newman, Anne B.; Rosano, Caterina] Univ Pittsburgh, Dept Epidemiol, 130 DeSoto St,127 Parran Hall, Pittsburgh, PA 15261 USA.
[Aizenstein, Howard J.] Univ Pittsburgh, Dept Psychiat, Pittsburgh, PA USA.
[Harris, Tamara] NIH, Lab Epidemiol Demog & Biometry, Bldg 10, Bethesda, MD 20892 USA.
[Satterfield, Suzanne] Univ Tennessee, Ctr Hlth Sci, Dept Prevent Med, Memphis, TN 38163 USA.
[Studenski, Stephanie A.] NIH, Longitudinal Studies Sect, Bldg 10, Bethesda, MD 20892 USA.
[Yaffe, Kristine] Univ Calif San Francisco, Dept Psychiat, San Francisco, CA USA.
RP Rosario, BL (reprint author), Univ Pittsburgh, Dept Epidemiol, 130 DeSoto St,127 Parran Hall, Pittsburgh, PA 15261 USA.
EM blr5@pitt.edu
FU National Institute on Aging (NIA) NIA [N01-AG-6-2101, N01-AG-6-2103,
N01-AG-6-2106, R01-AG-028050]; National Institute on Aging (NIA) NINR
[R01-NR-012459]; Intramural Research Program of the NIA [K23-AG-028966,
R01-AG-029232]; University of Pittsburgh Claude D. Pepper Older
Americans Independence Center [P30-AG-024827-07]; NIA [T32-AG-000181]
FX Health ABC was supported by the National Institute on Aging (NIA)
Contracts (N01-AG-6-2101, N01-AG-6-2103, N01-AG-6-2106, NIA grant
R01-AG-028050, and NINR grant R01-NR-012459). This research was
supported in part by the Intramural Research Program of the NIA
(K23-AG-028966 and R01-AG-029232), the University of Pittsburgh Claude
D. Pepper Older Americans Independence Center (P30-AG-024827-07), and a
training grant from the NIA (T32-AG-000181).
NR 31
TC 0
Z9 0
U1 2
U2 3
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 1079-5006
EI 1758-535X
J9 J GERONTOL A-BIOL
JI J. Gerontol. Ser. A-Biol. Sci. Med. Sci.
PD JUL
PY 2016
VL 71
IS 7
BP 968
EP 973
DI 10.1093/gerona/glv224
PG 6
WC Geriatrics & Gerontology; Gerontology
SC Geriatrics & Gerontology
GA DT0YW
UT WOS:000381209900018
PM 26755683
ER
PT J
AU Hong, SG
Lin, YS
Dunbar, CE
Zou, JZ
AF Hong, So Gun
Lin, Yongshun
Dunbar, Cynthia E.
Zou, Jizhong
TI The Role of Nonhuman Primate Animal Models in the Clinical Development
of Pluripotent Stem Cell Therapies
SO MOLECULAR THERAPY
LA English
DT News Item
ID REGENERATIVE MEDICINE; TRANSPLANTATION
C1 [Hong, So Gun; Dunbar, Cynthia E.] NHLBI, Hematol Branch, NIH, 10 Ctr Dr,Bldg 10 CRC,Room 4E-5132, Bethesda, MD 20892 USA.
[Lin, Yongshun; Zou, Jizhong] NHLBI, iPSC iore, Ctr Mol Med, NIH, Bldg 10, Bethesda, MD 20892 USA.
RP Dunbar, CE (reprint author), NHLBI, Hematol Branch, NIH, 10 Ctr Dr,Bldg 10 CRC,Room 4E-5132, Bethesda, MD 20892 USA.
EM dunbarc@nhlbi.nih.gov
NR 23
TC 0
Z9 0
U1 5
U2 5
PU NATURE PUBLISHING GROUP
PI NEW YORK
PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA
SN 1525-0016
EI 1525-0024
J9 MOL THER
JI Mol. Ther.
PD JUL
PY 2016
VL 24
IS 7
BP 1165
EP 1169
DI 10.1038/mt.2016.131
PG 5
WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine,
Research & Experimental
SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research &
Experimental Medicine
GA DT3YY
UT WOS:000381418300002
PM 27506376
ER
PT J
AU Wright, JT
Tampi, MP
Graham, L
Estrich, C
Crall, JJ
Fontana, M
Gillette, EJ
Novy, BB
Dhar, V
Donly, K
Hewlett, ER
Quinonez, RB
Chaffin, J
Crespin, M
Iafolla, T
Siegal, MD
Carrasco-Labra, A
AF Wright, John T.
Tampi, Malavika P.
Graham, Laurel
Estrich, Cameron
Crall, James J.
Fontana, Margherita
Gillette, E. Jane
Novy, Brian B.
Dhar, Vineet
Donly, Kevin
Hewlett, Edmond R.
Quinonez, Rocio B.
Chaffin, Jeffrey
Crespin, Matt
Iafolla, Timothy
Siegal, Mark D.
Carrasco-Labra, Alonso
TI Sealants for Preventing and Arresting Pit-and-fissure Occlusal Caries in
Primary and Permanent Molars A systematic review of randomized
controlled trials-a report of the American Academy of Pediatric
Dentistry and the American Dental Association
SO PEDIATRIC DENTISTRY
LA English
DT Review
DE GLASS IONOMER SEALANTS; RESIN-BASED SEALANTS; CARIES PREVENTION; CARIES
ARREST; PIT-AND-FISSURE SEALANTS; SYSTEMATIC REVIEW
ID GLASS-IONOMER; CLINICAL-EVALUATION; FLUORIDE VARNISH; RESIN SEALANT;
LOW-RISK; RETENTION
AB Background: National Health and Nutrition Examination Survey 2011-2012 data indicated that, in the United States, nearly one-fourth of children and over one-half of adolescents experienced dental caries in their permanent teeth. The purpose of this review was to summarize the available clinical evidence regarding the effect of dental sealants for the prevention and management of pit-and-fissure occlusal carious lesions in primary and permanent molars, compared with a control without sealants, with fluoride varnishes, or with other head-to head comparisons. Type of Studies Reviewed: The authors included parallel and split-mouth randomized controlled trials that included at least 2 years of follow-up, which they identified using MEDLINE (via PubMed), Embase, LILACS, the Cochrane Central Register of Controlled Trials, and registers of ongoing trials. Pairs of reviewers independently conducted the selection of studies, data extraction, risk of bias assessments, and quality of the evidence assessments by using the Grading of Recommendations Assessment, Development and Evaluation approach. Results: Of 2,869 records screened, the authors determined that 24 articles (representing 23 studies) proved eligible. Moderate-quality evidence suggested that participants who received sealants had a reduced risk of developing carious lesions in occlusal surfaces of permanent molars compared with those who did not receive sealants (odds ratio [OR], 0.15; 95% confidence interval [Cl] 0.08-027) after 7 or more years of follow-up. When the authors compared studies whose investigators had compared sealants with fluoride varnishes, they found that sealants reduced the incidence of carious lesions after 7 or more years of follow-up (OR, 0.19; 95% Cl, 0.07-0.51); however, this finding was supported by low-quality evidence. On the basis of the evidence, the authors could not provide a hierarchy of effectiveness among the studies whose investigators had conducted head-to-head comparisons. The investigators of 2 trials provided information about adverse events, but they did not report any adverse events. Conclusions and Practical Implications: Available evidence suggests that sealants are effective and safe to prevent or arrest the progression of noncavitated carious lesions compared with a control without sealants or fluoride varnishes. Further research is needed to provide information about the relative merits of the different types of sealant materials.
C1 [Wright, John T.] Univ N Carolina, Sch Dent, Dept Pediat Dent, Chapel Hill, NC USA.
[Tampi, Malavika P.] Amer Dent Assoc, Inst Sci, Ctr Evidence Based Dent, Chicago, IL 60611 USA.
[Graham, Laurel] Amer Acad Pediat Dent, Chicago, IL USA.
[Estrich, Cameron] Amer Dent Assoc, Inst Sci, Sci Informat, Chicago, IL USA.
[Crall, James J.] Univ Calif Los Angeles, Sch Dent, Div Publ Hlth & Community Dent, Los Angeles, CA 90024 USA.
[Fontana, Margherita] Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA.
[Gillette, E. Jane] Univ Washington, Sch Dent, Seattle, WA 98195 USA.
[Novy, Brian B.] DentaQuest Inst, Westborough, MA USA.
[Dhar, Vineet] Univ Maryland, Sch Dent, Div Pediat Dent, Baltimore, MD 21201 USA.
[Donly, Kevin] Univ Texas San Antonio, Hlth Sci Ctr, Dept Dev Dent, Sch Dent, San Antonio, TX USA.
[Hewlett, Edmond R.] Univ Calif Los Angeles, Sch Dent, Sect Restorat Dent, Los Angeles, CA 90024 USA.
[Quinonez, Rocio B.] Univ N Carolina, Dept Pediat Dent & Pediat, Sch Dent, Chapel Hill, NC USA.
[Chaffin, Jeffrey] Delta Dent Iowa, Johnston, IA USA.
[Chaffin, Jeffrey] AT Still Univ, Coll Grad Hlth Studies, Mesa, AZ USA.
[Chaffin, Jeffrey] Assoc State & Territorial Dent Directors, Reno, NV USA.
[Crespin, Matt] Childrens Hosp Wisconsin, Childrens Hlth Alliance Wisconsin, Milwaukee, WI USA.
[Iafolla, Timothy] Natl Inst Dent & Craniofacial Res, Program Anal & Reports Branch, NIH, Bethesda, MD USA.
[Siegal, Mark D.] Ohio State Univ, Coll Dent, Columbus, OH 43210 USA.
[Siegal, Mark D.] Amer Assoc Publ Hlth Dent, Springfield, IL USA.
[Carrasco-Labra, Alonso] Amer Dent Assoc, Ctr Evidence Based Dent, Chicago, IL USA.
[Carrasco-Labra, Alonso] Univ Chile, Evidence Based Dent Unit, Fac Dent, Santiago, Chile.
[Carrasco-Labra, Alonso] Univ Chile, Dept Oral & Maxillofacial Surg, Fac Dent, Santiago, Chile.
[Carrasco-Labra, Alonso] McMaster Univ, Dept Clin Epidemiol & Biostat, Hamilton, ON, Canada.
RP Tampi, MP (reprint author), Amer Dent Assoc, Inst Sci, Ctr Evidence Based Dent, Chicago, IL 60611 USA.
EM tampim@ada.org
FU National Institute of Dental; Craniofacial Research; Delta Dental;
Ivoclar Vivadent; Children's Dental Health Project; Delta Dental of
Wisconsin; Washington Dental Services Foundation; DentaQuest Foundation;
Health Resource and Services Administration Maternal and Child Health
Bureau; Healthier Wisconsin Partnership Program
FX Dr. Fontana is a consultant for the American Dental Association Council
on Scientific Affairs. In the past, she has received funds from the
National Institute of Dental and Craniofacial Research, Delta Dental,
and Ivoclar Vivadent to conduct research focused on dental sealants.
These grants ended before her engagement with the work involved in this
manuscript. Dr. Novy's previous continuing education lecture honoraria
were provided by the following manufacturers of sealant materials: GC
America, SDI, and Shofu, and his previous continuing education lecture
honoraria were provided by the following dental manufacturers: Air
Techniques, CariFree, GlaxoSmithKline, Ivoclar, Phillips, Solutionreach,
Triodent, and Xlear. Mr. Crespin is the chair of the Children's Dental
Health Project's sealant work group and has received funding from
Children's Dental Health Project, Delta Dental of Wisconsin, Washington
Dental Services Foundation, DentaQuest Foundation, Health Resource and
Services Administration Maternal and Child Health Bureau, and the
Healthier Wisconsin Partnership Program. Mr. Crespin serves on the board
of trustees of the American Dental Hygienists' Association. None of the
other authors reported any disclosures.
NR 44
TC 1
Z9 1
U1 3
U2 3
PU AMER ACAD PEDIATRIC DENTISTRY
PI CHICAGO
PA 211 E CHICAGO AVENUE SUITE 1036, CHICAGO, IL 60611-2616 USA
SN 0164-1263
EI 1942-5473
J9 PEDIATR DENT
JI Pediatr. Dent.
PD JUL-AUG
PY 2016
VL 38
IS 4
BP 282
EP 294
DI 10.1016/j.adaj.2016.06.003
PG 13
WC Dentistry, Oral Surgery & Medicine; Pediatrics
SC Dentistry, Oral Surgery & Medicine; Pediatrics
GA DT4KJ
UT WOS:000381448300003
PM 27557916
ER
PT J
AU Cabral, WA
Ishikawa, M
Garten, M
Makareeva, EN
Sargent, BM
Weis, M
Barnes, AM
Webb, EA
Shaw, NJ
Ala-Kokko, L
Lacbawan, FL
Hogler, W
Leikin, S
Blank, PS
Zimmerberg, J
Eyre, DR
Yamada, Y
Marini, JC
AF Cabral, Wayne A.
Ishikawa, Masaki
Garten, Matthias
Makareeva, Elena N.
Sargent, Brandi M.
Weis, MaryAnn
Barnes, Aileen M.
Webb, Emma A.
Shaw, Nicholas J.
Ala-Kokko, Leena
Lacbawan, Felicitas L.
Hogler, Wolfgang
Leikin, Sergey
Blank, Paul S.
Zimmerberg, Joshua
Eyre, David R.
Yamada, Yoshihiko
Marini, Joan C.
TI Absence of the ER Cation Channel TMEM38B/TRIC-B Disrupts Intracellular
Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive
Osteogenesis Imperfecta
SO PLOS GENETICS
LA English
DT Article
ID ENDOPLASMIC-RETICULUM STRESS; UNFOLDED-PROTEIN RESPONSE; I COLLAGEN;
CYCLOPHILIN-B; PROLYL 3-HYDROXYLATION; DELETION MUTATION; CHAIN
ASSOCIATION; LYSYL HYDROXYLASE; BONE-COLLAGEN; ALPHA 2(I)
AB Recessive osteogenesis imperfecta (OI) is caused by defects in proteins involved in post-translational interactions with type I collagen. Recently, a novel form of moderately severe OI caused by null mutations in TMEM38B was identified. TMEM38B encodes the ER membrane monovalent cation channel, TRIC-B, proposed to counterbalance IP3R-mediated Ca2+ release from intracellular stores. The molecular mechanisms by which TMEM38B mutations cause OI are unknown. We identified 3 probands with recessive defects in TMEM38B. TRIC-B protein is undetectable in proband fibroblasts and osteoblasts, although reduced TMEM38B transcripts are present. TRIC-B deficiency causes impaired release of ER luminal Ca2+, associated with deficient store-operated calcium entry, although SERCA and IP3R have normal stability. Notably, steady state ER Ca2+ is unchanged in TRIC-B deficiency, supporting a role for TRIC-B in the kinetics of ER calcium depletion and recovery. The disturbed Ca2+ flux causes ER stress and increased BiP, and dysregulates synthesis of proband type I collagen at multiple steps. Collagen helical lysine hydroxylation is reduced, while telopeptide hydroxylation is increased, despite increased LH1 and decreased Ca2+-dependent FKBP65, respectively. Although PDI levels are maintained, procollagen chain assembly is delayed in proband cells. The resulting misfolded collagen is substantially retained in TRIC-B null cells, consistent with a 50-70% reduction in secreted collagen. Lower-stability forms of collagen that elude proteasomal degradation are not incorporated into extracellular matrix, which contains only normal stability collagen, resulting in matrix insufficiency. These data support a role for TRIC-B in intracellular Ca2+ homeostasis, and demonstrate that absence of TMEM38B causes OI by dysregulation of calcium flux kinetics in the ER, impacting multiple collagen-specific chaperones and modifying enzymes.
C1 [Cabral, Wayne A.; Sargent, Brandi M.; Barnes, Aileen M.; Marini, Joan C.] NICHD, Sect Heritable Disorders Bone & Extracellular Mat, NIH, Bethesda, MD USA.
[Ishikawa, Masaki; Yamada, Yoshihiko] NIDCR, Mol Biol Sect, NIH, Bethesda, MD USA.
[Garten, Matthias; Blank, Paul S.; Zimmerberg, Joshua] NICHD, Sect Integrat Biophys, NIH, Bethesda, MD USA.
[Makareeva, Elena N.; Leikin, Sergey] NICHD, Sect Phys Biochem, NIH, Bethesda, MD USA.
[Weis, MaryAnn; Eyre, David R.] Univ Washington, Dept Orthopaed & Sports Med, Seattle, WA 98195 USA.
[Webb, Emma A.; Hogler, Wolfgang] Univ Birmingham, Sch Clin & Expt Med, Inst Biomed Res, Birmingham, W Midlands, England.
[Webb, Emma A.; Shaw, Nicholas J.; Hogler, Wolfgang] Birmingham Childrens Hosp, Dept Endocrinol & Diabet, Birmingham, W Midlands, England.
[Ala-Kokko, Leena] Connect Tissue Gene Tests, Allentown, PA USA.
[Lacbawan, Felicitas L.] Childrens Natl Med Ctr, Dept Med Genet, Washington, DC 20010 USA.
RP Marini, JC (reprint author), NICHD, Sect Heritable Disorders Bone & Extracellular Mat, NIH, Bethesda, MD USA.
EM oidoc@helix.nih.gov
RI Leikin, Sergey/A-5518-2008;
OI Leikin, Sergey/0000-0001-7095-0739; Garten, Matthias/0000-0002-8660-1039
FU NIH [AR037318, HD070394]; NIH
FX This study was supported by NIH grants AR037318 (NIAMS) and HD070394
(NICHD) to DRE, and NIH intramural funds to YY (NIDCR), SL (NICHD), JZ
(NICHD) and JCM (NICHD). The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the
manuscript.
NR 69
TC 3
Z9 3
U1 4
U2 4
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-7404
J9 PLOS GENET
JI PLoS Genet.
PD JUL
PY 2016
VL 12
IS 7
AR e1006156
DI 10.1371/journal.pgen.1006156
PG 27
WC Genetics & Heredity
SC Genetics & Heredity
GA DS8RI
UT WOS:000381050100024
PM 27441836
ER
PT J
AU De, S
Mitra, A
Cheng, YZ
Pfeifer, K
Kassis, JA
AF De, Sandip
Mitra, Apratim
Cheng, Yuzhong
Pfeifer, Karl
Kassis, Judith A.
TI Formation of a Polycomb-Domain in the Absence of Strong Polycomb
Response Elements
SO PLOS GENETICS
LA English
DT Article
ID DROSOPHILA ENGRAILED GENE; IN-SITU DISSECTION; BITHORAX COMPLEX;
REGULATORY SEQUENCES; MELANOGASTER; PROTEINS; DNA; REPRESSION; BINDING;
EXPRESSION
AB Polycomb group response elements (PREs) in Drosophila are DNA-elements that recruit Polycomb proteins (PcG) to chromatin and regulate gene expression. PREs are easily recognizable in the Drosophila genome as strong peaks of PcG-protein binding over discrete DNA fragments; many small but statistically significant PcG peaks are also observed in PcG domains. Surprisingly, in vivo deletion of the four characterized strong PREs from the PcG regulated invected-engrailed (inv-en) gene complex did not disrupt the formation of the H3K27me3 domain and did not affect inv-en expression in embryos or larvae suggesting the presence of redundant PcG recruitment mechanism. Further, the 3D-structure of the inv-en domain was only minimally altered by the deletion of the strong PREs. A reporter construct containing a 7.5kb en fragment that contains three weak peaks but no large PcG peaks forms an H3K27me3 domain and is PcG-regulated. Our data suggests a model for the recruitment of PcG-complexes to Drosophila genes via interactions with multiple, weak PREs spread throughout an H3K27me3 domain.
C1 [De, Sandip; Mitra, Apratim; Cheng, Yuzhong; Pfeifer, Karl; Kassis, Judith A.] Eunice Kennedy Shriver Natl Inst Child Hlth Human, Program Genom Differentiat, NIH, Bethesda, MD 20892 USA.
RP Kassis, JA (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth Human, Program Genom Differentiat, NIH, Bethesda, MD 20892 USA.
EM jkassis@mail.nih.gov
OI Pfeifer, Karl/0000-0002-0254-682X
FU Division of Intramural Research, Eunice Kennedy Shriver National
Institute of Child Health and Human Development, National Institutes of
Health
FX This work was supported by the Division of Intramural Research, Eunice
Kennedy Shriver National Institute of Child Health and Human
Development, National Institutes of Health. The funders had no role in
study design, data collection and analysis, decision to publish, or
preparation of the manuscript.
NR 71
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U1 7
U2 7
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-7404
J9 PLOS GENET
JI PLoS Genet.
PD JUL
PY 2016
VL 12
IS 7
AR e1006200
DI 10.1371/journal.pgen.1006200
PG 22
WC Genetics & Heredity
SC Genetics & Heredity
GA DS8RI
UT WOS:000381050100055
PM 27466807
ER
PT J
AU Xiao, Y
Thoresen, DT
Miao, LL
Williams, JS
Wang, CC
Atit, RP
Wong, SY
Brownell, I
AF Xiao, Ying
Thoresen, Daniel T.
Miao, Lingling
Williams, Jonathan S.
Wang, Chaochen
Atit, Radhika P.
Wong, Sunny Y.
Brownell, Isaac
TI A Cascade of Wnt, Eda, and Shh Signaling Is Essential for Touch Dome
Merkel Cell Development
SO PLOS GENETICS
LA English
DT Article
ID HAIR FOLLICLE MORPHOGENESIS; EPIDERMAL STEM-CELLS; SONIC-HEDGEHOG;
EMBRYONIC-DEVELOPMENT; BETA-CATENIN; DORSAL SKIN; MOUSE SKIN; MICE;
EXPRESSION; ROLES
AB The Sonic hedgehog (Shh) signaling pathway regulates developmental, homeostatic, and repair processes throughout the body. In the skin, touch domes develop in tandem with primary hair follicles and contain sensory Merkel cells. The developmental signaling requirements for touch dome specification are largely unknown. We found dermal Wnt signaling and subsequent epidermal Eda/Edar signaling promoted Merkel cell morphogenesis by inducing Shh expression in early follicles. Lineage-specific gene deletions revealed intraepithelial Shh signaling was necessary for Merkel cell specification. Additionally, a Shh signaling agonist was sufficient to rescue Merkel cell differentiation in Edar-deficient skin. Moreover, Merkel cells formed in Fgf20 mutant skin where primary hair formation was defective but Shh production was preserved. Although developmentally associated with hair follicles, fate mapping demonstrated Merkel cells primarily originated outside the hair follicle lineage. These findings suggest that touch dome development requires Wnt-dependent mesenchymal signals to establish reciprocal signaling within the developing ectoderm, including Eda signaling to primary hair placodes and ultimately Shh signaling from primary follicles to extrafollicular Merkel cell progenitors. Shh signaling often demonstrates pleiotropic effects within a structure over time. In postnatal skin, Shh is known to regulate the self-renewal, but not the differentiation, of touch dome stem cells. Our findings relate the varied effects of Shh in the touch dome to the ligand source, with locally produced Shh acting as a morphogen essential for lineage specification during development and neural Shh regulating postnatal touch dome stem cell maintenance.
C1 [Xiao, Ying; Thoresen, Daniel T.; Miao, Lingling; Williams, Jonathan S.; Brownell, Isaac] Natl Canc Inst, Dermatol Branch, Ctr Canc Res, NIH, Bethesda, MD USA.
[Wang, Chaochen] NIDDK, Lab Genet & Physiol, NIH, Bethesda, MD 20892 USA.
[Atit, Radhika P.] Case Western Reserve Univ, Dept Biol, Cleveland, OH 44106 USA.
[Wong, Sunny Y.] Univ Michigan, Dept Dermatol, Ann Arbor, MI 48109 USA.
[Wong, Sunny Y.] Univ Michigan, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA.
RP Brownell, I (reprint author), Natl Canc Inst, Dermatol Branch, Ctr Canc Res, NIH, Bethesda, MD USA.
EM isaac.brownell@nih.gov
OI Brownell, Isaac/0000-0002-0090-9914
FU NIH Intramural Research Program, CCR, NCI [ZIA BC011393]; NIH-NIDCR
[R01DE01870]; NIH-NIAMS [R01AR065409]
FX This work was supported by the NIH Intramural Research Program, CCR,
NCI. Award number ZIA BC011393 to IB. https://ccr.cancer.gov/. NIH-NIDCR
award number R01DE01870 to RPA. http://www.nidcr.nih.gov/. NIH-NIAMS
award number R01AR065409 to SYW. http://www.niams.nih.gov/. The funders
had no role in study design, data collection and analysis, decision to
publish, or preparation of the manuscript.
NR 53
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U1 2
U2 3
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-7404
J9 PLOS GENET
JI PLoS Genet.
PD JUL
PY 2016
VL 12
IS 7
AR e1006150
DI 10.1371/journal.pgen.1006150
PG 19
WC Genetics & Heredity
SC Genetics & Heredity
GA DS8RI
UT WOS:000381050100019
PM 27414798
ER
PT J
AU Zhao, XN
Lokanga, R
Allette, K
Gazy, I
Wu, D
Usdin, K
AF Zhao, Xiao-Nan
Lokanga, Rachel
Allette, Kimaada
Gazy, Inbal
Wu, Di
Usdin, Karen
TI A MutS beta-Dependent Contribution of MutS alpha to Repeat Expansions in
Fragile X Premutation Mice?
SO PLOS GENETICS
LA English
DT Article
ID MISMATCH-REPAIR PROTEINS; KNOCK-IN MICE; MOUSE MODEL; TRINUCLEOTIDE
REPEATS; SOMATIC EXPANSION; TRIPLET-REPEAT; DNA-REPAIR; CANCER
SUSCEPTIBILITY; HAIRPIN STRUCTURES; TRANSGENIC MICE
AB The fragile X-related disorders result from expansion of a CGG/CCG microsatellite in the 5' UTR of the FMR1 gene. We have previously demonstrated that the MSH2/MSH3 complex, MutS beta, that is important for mismatch repair, is essential for almost all expansions in a mouse model of these disorders. Here we show that the MSH2/MSH6 complex, MutS alpha also contributes to the production of both germ line and somatic expansions as evidenced by the reduction in the number of expansions observed in Msh6(-/-) mice. This effect is not mediated via an indirect effect of the loss of MSH6 on the level of MSH3. However, since MutS beta is required for 98% of germ line expansions and almost all somatic ones, MutS alpha is apparently not able to efficiently substitute for MutS beta in the expansion process. Using purified human proteins we demonstrate that MutS alpha, like MutS beta, binds to substrates with loop-outs of the repeats and increases the thermal stability of the structures that they form. We also show that MutS alpha facilitates binding of MutS beta to these loop-outs. These data suggest possible models for the contribution of MutS alpha to repeat expansion. In addition, we show that unlike MutS beta, MutS alpha may also act to protect against repeat contractions in the Fmr1 gene.
C1 [Zhao, Xiao-Nan; Lokanga, Rachel; Allette, Kimaada; Gazy, Inbal; Usdin, Karen] NIDDKD, Sect Gene Struct & Dis, Lab Cell & Mol Biol, NIH, Bethesda, MD 20892 USA.
[Lokanga, Rachel] Univ Cape Town, Sch Med, Div Med Biochem, Cape Town, South Africa.
[Wu, Di] NIDDKD, Sect Phys Biochem, Lab Biochem & Genet, NIH, Bethesda, MD 20892 USA.
[Allette, Kimaada] Icahn Sch Med Mt Sinai, Icahn Inst, New York, NY 10029 USA.
[Allette, Kimaada] Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY 10029 USA.
[Wu, Di] Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USA.
RP Usdin, K (reprint author), NIDDKD, Sect Gene Struct & Dis, Lab Cell & Mol Biol, NIH, Bethesda, MD 20892 USA.
EM ku@helix.nih.gov
RI Zhao, Xiaonan/S-3139-2016
FU National Institute of Diabetes, Digestive and Kidney Diseases
[DK057808-07]
FX The work described in this manuscript was funded by a grant from the
Intramural Program of the National Institute of Diabetes, Digestive and
Kidney Diseases to KU (Grant number DK057808-07). The funders had no
role in study design, data collection and analysis, decision to publish,
or preparation of the manuscript.
NR 55
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U1 1
U2 1
PU PUBLIC LIBRARY SCIENCE
PI SAN FRANCISCO
PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA
SN 1553-7404
J9 PLOS GENET
JI PLoS Genet.
PD JUL
PY 2016
VL 12
IS 7
AR e1006190
DI 10.1371/journal.pgen.1006190
PG 19
WC Genetics & Heredity
SC Genetics & Heredity
GA DS8RI
UT WOS:000381050100049
PM 27427765
ER
PT J
AU Liu, H
Ning, J
Qin, J
Shen, Y
AF Liu, Hao
Ning, Jing
Qin, Jing
Shen, Yu
TI SEMIPARAMETRIC MAXIMUM LIKELIHOOD INFERENCE FOR TRUNCATED OR
BIASED-SAMPLING DATA
SO STATISTICA SINICA
LA English
DT Article
DE Biased sampling; length bias data; truncated and right censored survival
data
ID PREVALENT COHORT DATA; SELECTION BIAS; SURVIVAL-DATA;
NONPARAMETRIC-ESTIMATION; REGRESSION-ANALYSIS; HAZARDS REGRESSION;
STATISTICAL-MODELS; ASYMPTOTIC THEORY; FRAILTY MODEL; CENSORED-DATA
AB Sample selection bias has long been recognized in many fields including clinical trials, epidemiology studies, genome-wide association studies, and wildlife management. This paper investigates the maximum likelihood estimation for censored survival data with selection bias under the Cox regression models where the selection process is modeled parametrically. A novel expectation-maximization algorithm is proposed and shown to have considerable computational advantages. Rigorous asymptotic properties of the estimator are established. Extensive simulation studies and a data analysis are conducted to investigate the performance of the proposed estimation procedure.
C1 [Liu, Hao] Baylor Coll Med, Div Biostat, Dan L Duncan Canc Ctr, Houston, TX 77030 USA.
[Ning, Jing; Shen, Yu] Univ Texas MD Anderson Canc Ctr, Dept Biostat, Houston, TX 77030 USA.
[Qin, Jing] NIAID, Biostat Res Branch, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.
RP Liu, H (reprint author), Baylor Coll Med, Div Biostat, Dan L Duncan Canc Ctr, Houston, TX 77030 USA.
EM haol@bcm.edu; jning@mdanderson.org; jingqin@niaid.nih.gov;
yshen@mdanderson.org
FU Seniors' Independence Research Program, through the National Health
Research and Development Program of Health Canada [6606-3954-MC(S)];
Pfizer Canada Incorporated through the Medical Research
Council/Pharmaceutical Manufacturers Association of Canada Health
Activity Program, NHRDP [6603-1417-302(R)]; Bayer Incorporated; British
Columbia Health Research Foundation Projects [38 (93-2), 34 (96-1)];
National Institutes of Health
FX We thank Professor Masoud Asgharian and the investigators of the
Canadian Study of Health and Aging (CHSA) for providing us with the
dementia data, which were collected as part of the CHSA, funded by the
Seniors' Independence Research Program, through the National Health
Research and Development Program of Health Canada (Project
no.6606-3954-MC(S)). Additional funding was provided by Pfizer Canada
Incorporated through the Medical Research Council/Pharmaceutical
Manufacturers Association of Canada Health Activity Program, NHRDP
Project 6603-1417-302(R), Bayer Incorporated, and the British Columbia
Health Research Foundation Projects 38 (93-2) and 34 (96-1). The study
was coordinated through the University of Ottawa and the Division of
Aging and Seniors, Health Canada. This work in this paper was supported
in part by the National Institutes of Health.
NR 47
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U1 0
U2 0
PU STATISTICA SINICA
PI TAIPEI
PA C/O DR H C HO, INST STATISTICAL SCIENCE, ACADEMIA SINICA, TAIPEI 115,
TAIWAN
SN 1017-0405
EI 1996-8507
J9 STAT SINICA
JI Stat. Sin.
PD JUL
PY 2016
VL 26
IS 3
BP 1087
EP 1115
DI 10.5705/ss.2014.094
PG 29
WC Statistics & Probability
SC Mathematics
GA DT6JU
UT WOS:000381591300010
ER
PT J
AU Jiang, SZ
Eiden, LE
AF Jiang, Sunny Zhihong
Eiden, Lee E.
TI Activation of the HPA axis and depression of feeding behavior induced by
restraint stress are separately regulated by PACAPergic
neurotransmission in the mouse
SO STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS
LA English
DT Article; Proceedings Paper
CT 11th International Symposium on Catecholamines and Other
Neurotransmitters in Stress
CY JUN, 2015
CL SLOVAKIA
DE Acute psychogenic stress; chronic psychogenic stress; corticosterone;
HPA axis; hypophagia; pituitary adenylate cyclase-activating
polypeptide; prolonged psychogenic stress; restraint stress
ID HYPOTHALAMIC PARAVENTRICULAR NUCLEUS; STRIA TERMINALIS BNST;
PITUITARY-ADRENOCORTICAL AXIS; CENTRAL-NERVOUS-SYSTEM; HORMONE CRH GENE;
POLYPEPTIDE PACAP; BED NUCLEUS; EMOTIONAL-STRESS; PEPTIDE PACAP;
BRAIN-STEM
AB We measured serum CORT elevation in wild-type and PACAP-deficient C57BL/6N male mice after acute (1h) or prolonged (2-3h) daily restraint stress for 7 d. The PACAP dependence of CORT elevation was compared to that of stress-induced hypophagia. Daily restraint induced unhabituated peak CORT elevation, and hypophagia/weight loss, of similar magnitude for 1, 2, and 3h of daily restraint, in wild-type mice. Peak CORT elevation, and hypophagia, were both attenuated in PACAP-deficient mice for 2 and 3h daily restraint. Hypophagia induced by 1-h daily restraint was also greatly reduced in PACAP-deficient mice, however CORT elevation, both peak and during recovery from stress, was unaffected. Thus, hypothalamic PACAPergic neurotransmission appears to affect CRH gene transcription and peptide production, but not CRH release, in response to psychogenic stress. A single exposure to restraint sufficed to trigger hypophagia over the following 24h. PACAP deficiency attenuated HPA axis response (CORT elevation) to prolonged (3h) but not acute (1h) single-exposure restraint stress, while hypophagia induced by either a single 1h or a single 3h restraint were both abolished in PACAP-deficient mice. These results suggest that PACAP's actions to promote suppression of food intake following an episode of psychogenic stress is unrelated to the release of CRH into the portal circulation to activate the pituitary-adrenal axis. Furthermore, demonstration of suppressed food intake after a single 1-h restraint stress provides a convenient assay for investigating the location of the synapses and circuits mediating the effects of PACAP on the behavioral sequelae of psychogenic stress.
C1 [Jiang, Sunny Zhihong; Eiden, Lee E.] NIMH, Sect Mol Neurosci, Bldg 49,Room 5A-38,9000 Rockville Pike, Bethesda, MD 20892 USA.
RP Eiden, LE (reprint author), NIMH, Sect Mol Neurosci, Bldg 49,Room 5A-38,9000 Rockville Pike, Bethesda, MD 20892 USA.
EM eidenl@mail.nih.gov
FU NIMH NIH HHS [Z01 MH002386]
NR 42
TC 1
Z9 1
U1 0
U2 0
PU TAYLOR & FRANCIS LTD
PI ABINGDON
PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND
SN 1025-3890
EI 1607-8888
J9 STRESS
JI Stress
PD JUL
PY 2016
VL 19
IS 4
BP 374
EP 382
DI 10.1080/10253890.2016.1174851
PG 9
WC Behavioral Sciences; Endocrinology & Metabolism; Neurosciences
SC Behavioral Sciences; Endocrinology & Metabolism; Neurosciences &
Neurology
GA DU9EF
UT WOS:000382518400005
PM 27228140
ER
PT J
AU Krizanova, O
Babula, P
Pacak, K
AF Krizanova, O.
Babula, P.
Pacak, K.
TI Stress, catecholaminergic system and cancer
SO STRESS-THE INTERNATIONAL JOURNAL ON THE BIOLOGY OF STRESS
LA English
DT Article; Proceedings Paper
CT 11th International Symposium on Catecholamines and Other
Neurotransmitters in Stress
CY JUN, 2015
CL SLOVAKIA
DE Adrenergic receptors; -blockers; catecholamines; dopamine; epinephrine;
norepinephrine; stress; tumor
ID SQUAMOUS-CELL CARCINOMA; REPEATED IMMOBILIZATION STRESS; ENDOTHELIAL
GROWTH-FACTOR; POPULATION-BASED COHORT; OVARIAN-CARCINOMA;
BREAST-CANCER; COLORECTAL-CANCER; GASTRIC-CANCER; BETA-BLOCKERS;
TUMOR-GROWTH
AB Stress as a modern civilization factor significantly affects our lives. While acute stress might have a positive effect on the organism, chronic stress is usually detrimental and might lead to serious health complications. It is known that stress induced by the physical environment (temperature-induced cold stress) can significantly impair the efficacy of cytotoxic chemotherapies and the anti-tumor immune response. On the other hand, epidemiological evidence has shown that patients taking drugs known as -adrenergic antagonists (-blockers), which are commonly prescribed to treat arrhythmia, hypertension, and anxiety, have significantly lower rates of several cancers. In this review, we summarize the current knowledge about catecholamines as important stress hormones in tumorigenesis and discuss the use of -blockers as the potential therapeutic agents.
C1 [Krizanova, O.] Slovak Acad Sci, Inst Clin & Translat Res, Biomed Res Ctr, Dubravska Cesta 9, Bratislava 84505, Slovakia.
[Krizanova, O.; Babula, P.] Masaryk Univ, Dept Physiol, Fac Med, Brno, Czech Republic.
[Pacak, K.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Dev Endocrinol & Tumor Genet Affin Grp, Sect Med Neuroendocrinol, NIH, Bethesda, MD USA.
RP Krizanova, O (reprint author), Slovak Acad Sci, Inst Clin & Translat Res, Biomed Res Ctr, Dubravska Cesta 9, Bratislava 84505, Slovakia.
EM olga.krizanova@savba.sk
NR 112
TC 2
Z9 2
U1 11
U2 11
PU TAYLOR & FRANCIS LTD
PI ABINGDON
PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND
SN 1025-3890
EI 1607-8888
J9 STRESS
JI Stress
PD JUL
PY 2016
VL 19
IS 4
BP 419
EP 428
DI 10.1080/10253890.2016.1203415
PG 10
WC Behavioral Sciences; Endocrinology & Metabolism; Neurosciences
SC Behavioral Sciences; Endocrinology & Metabolism; Neurosciences &
Neurology
GA DU9EF
UT WOS:000382518400010
PM 27398826
ER
PT J
AU Stephens, ML
Betts, K
Beck, NB
Cogliano, V
Dickersin, K
Fitzpatrick, S
Freeman, J
Gray, G
Hartung, T
McPartland, J
Rooney, AA
Scherer, RW
Verloo, D
Hoffmann, S
AF Stephens, Martin L.
Betts, Kellyn
Beck, Nancy B.
Cogliano, Vincent
Dickersin, Kay
Fitzpatrick, Suzanne
Freeman, James
Gray, George
Hartung, Thomas
McPartland, Jennifer
Rooney, Andrew A.
Scherer, Roberta W.
Verloo, Didier
Hoffmann, Sebastian
TI The Emergence of Systematic Review in Toxicology
SO TOXICOLOGICAL SCIENCES
LA English
DT Review
DE systematic review; risk of bias; data integration
ID EVIDENCE-BASED MEDICINE; ENVIRONMENTAL-HEALTH SCIENCE; FETAL-GROWTH;
EPIDEMIOLOGIC EVIDENCE; ARSENIC EXPOSURE; QUALITY; INTEGRATION;
VALIDATION
AB The Evidence-based Toxicology Collaboration hosted a workshop on "The Emergence of Systematic Review and Related Evidence-based Approaches in Toxicology," on November 21, 2014 in Baltimore, Maryland. The workshop featured speakers from agencies and organizations applying systematic review approaches to questions in toxicology, speakers with experience in conducting systematic reviews in medicine and healthcare, and stakeholders in industry, government, academia, and non-governmental organizations. Based on the workshop presentations and discussion, here we address the state of systematic review methods in toxicology, historical antecedents in both medicine and toxicology, challenges to the translation of systematic review from medicine to toxicology, and thoughts on the way forward. We conclude with a recommendation that as various agencies and organizations adapt systematic review methods, they continue to work together to ensure that there is a harmonized process for how the basic elements of systematic review methods are applied in toxicology.
C1 [Stephens, Martin L.; Hartung, Thomas] Johns Hopkins Ctr Alternat Anim Testing, Baltimore, MD USA.
[Beck, Nancy B.] Amer Chem Council, Washington, DC USA.
[Cogliano, Vincent] US EPA, Arlington, VA USA.
[Dickersin, Kay; Scherer, Roberta W.] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Baltimore, MD USA.
[Fitzpatrick, Suzanne] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD USA.
[Freeman, James] ExxonMobil Biomed Sci, Annandale, NJ USA.
[Gray, George] George Washington Univ, Milken Inst Sch Publ Hlth, Washington, DC USA.
[Hartung, Thomas] Univ Konstanz, CAAT Europe, Constance, Germany.
[McPartland, Jennifer] Environm Def Fund, Washington, DC USA.
[Rooney, Andrew A.] NIEHS, Off Hlth Assessment & Translat, Div Natl Toxicol Program, NIH, POB 12233, Res Triangle Pk, NC 27709 USA.
[Verloo, Didier] European Food Safety Author, I-43126 Parma, Italy.
[Hoffmann, Sebastian] Seh Consulting Serv, D-33098 Paderborn, Germany.
RP Stephens, ML (reprint author), Johns Hopkins Ctr Alternat Anim Testing, Baltimore, MD USA.
EM msteph14@jhu.edu
FU ExxonMobil Foundation
FX This work was supported by funding from an anonymous private charitable
foundation and the ExxonMobil Foundation to the Johns Hopkins Center for
Alternatives to Animal Testing (CAAT). CAAT serves as the secretariat
for the Evidence-based Toxicology Collaboration, which hosted the
workshop on which this article is based. Only those authors working at
or for CAAT (K.B., T.H., S.H., and M.L.S.) received compensation from
these sources via CAAT for their participation in the workshop or their
preparation of this article.
NR 44
TC 4
Z9 4
U1 4
U2 7
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 1096-6080
EI 1096-0929
J9 TOXICOL SCI
JI Toxicol. Sci.
PD JUL
PY 2016
VL 152
IS 1
BP 10
EP 16
DI 10.1093/toxsci/kfw059
PG 7
WC Toxicology
SC Toxicology
GA DT2AA
UT WOS:000381282000002
PM 27208075
ER
PT J
AU El-Masri, H
Kleinstreuer, N
Hines, RN
Adams, L
Tal, T
Isaacs, K
Wetmore, BA
Tan, YM
AF El-Masri, Hisham
Kleinstreuer, Nicole
Hines, Ronald N.
Adams, Linda
Tal, Tamara
Isaacs, Kristin
Wetmore, Barbara A.
Tan, Yu-Mei
TI Integration of Life-Stage Physiologically Based Pharmacokinetic Models
with Adverse Outcome Pathways and Environmental Exposure Models to
Screen for Environmental Hazards
SO TOXICOLOGICAL SCIENCES
LA English
DT Article
DE PBPK; AOPs; life-stage; developmental toxicology; environmental
toxicology
ID BLOOD-FLOW; IN-VITRO; PBPK MODELS; HUMAN FETUS; DEVELOPMENTAL
EXPRESSION; TOXCAST PROGRAM; CARDIAC-OUTPUT; DUCTUS VENOSUS; TOXICITY;
DOSIMETRY
AB A computational framework was developed to assist in screening and prioritizing chemicals based on their dosimetry, toxicity, and potential exposures. The overall strategy started with contextualizing chemical activity observed in high-throughput toxicity screening (HTS) by mapping these assays to biological events described in Adverse Outcome Pathways (AOPs). Next, in vitro to in vivo (IVIVE) extrapolation was used to convert an in vitro dose to an external exposure level, which was compared with potential exposure levels to derive an AOP-based margins of exposure (MOE). In this study, the framework was applied to estimate MOEs for chemicals that can potentially cause developmental toxicity following a putative AOP for fetal vasculogenesis/angiogenesis. A physiologically based pharmacokinetic (PBPK) model was developed to describe chemical disposition during pregnancy, fetal, neonatal, and infant to adulthood stages. Using this life-stage PBPK model, maternal exposures were estimated that would yield fetal blood levels equivalent to the chemical concentration that altered in vitro activity of selected HTS assays related to the most sensitive vasculogenesis/angiogenesis putative AOP. The resulting maternal exposure estimates were then compared with potential exposure levels using literature data or exposure models to derive AOP-based MOEs.
C1 [El-Masri, Hisham; Hines, Ronald N.; Adams, Linda; Tal, Tamara] US EPA, Natl Human & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC USA.
[Kleinstreuer, Nicole] Natl Inst Environm Hlth Sci, Natl Toxicol Program, Interagency Ctr Evaluat Alternat Toxicol Methods, Res Triangle Pk, NC USA.
[Isaacs, Kristin; Tan, Yu-Mei] US EPA, Natl Exposure Res Lab, Off Res & Dev, Res Triangle Pk, NC USA.
[Wetmore, Barbara A.] ScitoVation, Res Triangle Pk, NC USA.
RP El-Masri, H (reprint author), US EPA, Natl Human & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC USA.
EM el-masri.hisham@epa.gov
OI Kleinstreuer, Nicole/0000-0002-7914-3682; Tal,
Tamara/0000-0001-8365-9385
NR 46
TC 0
Z9 0
U1 6
U2 7
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 1096-6080
EI 1096-0929
J9 TOXICOL SCI
JI Toxicol. Sci.
PD JUL
PY 2016
VL 152
IS 1
BP 230
EP 243
DI 10.1093/toxsci/kfw082
PG 14
WC Toxicology
SC Toxicology
GA DT2AA
UT WOS:000381282000020
PM 27208077
ER
PT J
AU Balandaram, G
Kramer, LR
Kang, BH
Murray, LA
Perdew, GH
Gonzalez, FJ
Peters, JM
AF Balandaram, Gayathri
Kramer, Lance R.
Kang, Boo-Hyon
Murray, Lain A.
Perdew, Gary H.
Gonzalez, Frank J.
Peters, Jeffrey M.
TI Ligand activation of peroxisome proliferator-activated
receptor-beta/delta suppresses liver tumorigenesis in hepatitis B
transgenic mice
SO TOXICOLOGY
LA English
DT Article
DE Peroxisome proliferator-activated receptor-beta/delta; Liver cancer;
Kupffer cell; Hepatitis B; Inflammation
ID HUMAN HEPATOCELLULAR-CARCINOMA; NECROSIS-FACTOR-ALPHA; E-2 SIGNALING
PATHWAYS; CHOLINE-DEFICIENT DIET; PPAR-DELTA AGONIST; C VIRUS-INFECTION;
GENE-EXPRESSION; CYCLIN D1; MOLECULAR PATHOGENESIS; METHIONINE-DEFICIENT
AB Peroxisome proliferator-activated receptor-beta/delta (PPAR beta/delta) inhibits steatosis and inflammation, known risk factors for liver cancer. In this study, the effect of ligand activation of PPAR beta/delta in modulating liver tumorigenesis in transgenic hepatitis B virus (HBV) mice was examined. Activation of PPAR beta/delta in HBV mice reduced steatosis, the average number of liver foci, and tumor multiplicity. Reduced expression of hepatic CYCLIN D1 and c-MYC, tumor necrosis factor alpha (Tnfa) mRNA, serum levels of alanine aminotransaminase, and an increase in apoptotic signaling was also observed following ligand activation of PPAR beta/delta in HBV mice compared to controls. Inhibition of Tnfa mRNA expression was not observed in wild-type hepatocytes. Ligand activation of PPAR beta/delta inhibited lipopolysaccharide (LPS)-induced mRNA expression of Tnfa in wild-type, but not in Ppar beta/delta-null Kupffer cells. Interestingly, LPS-induced expression of Tnfa mRNA was also inhibited in Kupffer cells from a transgenic mouse line that expressed a DNA binding mutant form of PPAR beta/delta compared to controls. Combined, these results suggest that ligand activation of PPAR beta/delta attenuates hepatic tumorigenesis in HBV transgenic mice by inhibiting steatosis and cell proliferation, enhancing hepatocyte apoptosis, and modulating anti-inflammatory activity in Kupffer cells. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
C1 [Balandaram, Gayathri; Kramer, Lance R.; Murray, Lain A.; Perdew, Gary H.; Peters, Jeffrey M.] Penn State Univ, Dept Vet & Biomed Sci, 312 Life Sci Bldg, University Pk, PA 16802 USA.
[Balandaram, Gayathri; Kramer, Lance R.; Murray, Lain A.; Perdew, Gary H.; Peters, Jeffrey M.] Penn State Univ, Ctr Mol Toxicol & Carcinogenesis, 312 Life Sci Bldg, University Pk, PA 16802 USA.
[Kang, Boo-Hyon] Chemon Nonclin Res Inst, 240 Nampyeong Ro, Yongin, Gyeonggi Do, South Korea.
[Gonzalez, Frank J.] NCI, Lab Metab, Bethesda, MD 20892 USA.
RP Peters, JM (reprint author), Penn State Univ, Dept Vet & Biomed Sci, 312 Life Sci Bldg, University Pk, PA 16802 USA.; Peters, JM (reprint author), Penn State Univ, Ctr Mol Toxicol & Carcinogenesis, 312 Life Sci Bldg, University Pk, PA 16802 USA.
EM jmp21@psu.edu
FU National Cancer Institute of the National Institutes of Health
[CA140369, CA124533]
FX The authors gratefully acknowledge Cherie Anderson from the Penn State
Transgenic Mouse Facility, University Park, PA. for generating the
transgenic Ppar beta/delta DNA binding domain mutant mouse line.
Research reported in this publication was supported in part by the
National Cancer Institute of the National Institutes of Health under
Award Numbers CA140369 and CA124533. The content is solely the
responsibility of the authors and does not necessarily represent the
official views of the National Institutes of Health.
NR 78
TC 1
Z9 1
U1 0
U2 0
PU ELSEVIER IRELAND LTD
PI CLARE
PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000,
IRELAND
SN 0300-483X
J9 TOXICOLOGY
JI Toxicology
PD JUL 1
PY 2016
VL 363
BP 1
EP 9
DI 10.1016/j.tox.2016.07.010
PG 9
WC Pharmacology & Pharmacy; Toxicology
SC Pharmacology & Pharmacy; Toxicology
GA DU7PX
UT WOS:000382408200001
PM 27427494
ER
PT J
AU Remy, KE
Sun, J
Wang, D
Welsh, J
Solomon, SB
Klein, HG
Natanson, C
Cortes-Puch, I
AF Remy, K. E.
Sun, J.
Wang, D.
Welsh, J.
Solomon, S. B.
Klein, H. G.
Natanson, C.
Cortes-Puch, I.
TI Transfusion of recently donated (fresh) red blood cells (RBCs) does not
improve survival in comparison with current practice, while safety of
the oldest stored units is yet to be established: a meta-analysis
SO VOX SANGUINIS
LA English
DT Article
DE blood safety; clinical trial; quality control; red cell components;
transfusion medicine (in general); transfusion therapy
ID IN-HOSPITAL MORTALITY; CARDIAC-SURGERY; STORAGE DURATION;
CRITICALLY-ILL; PROLONGED STORAGE; RANDOMIZED-TRIAL; AGE; OUTCOMES;
TRAUMA; ASSOCIATION
AB Background and Objectives Preclinical studies generated the hypothesis that older stored red blood cells (RBCs) can increase transfusion risks. To examine the most updated and complete clinical evidence and compare results between two trial designs, we assessed both observational studies and randomized controlled trials (RCTs) studying the effect of RBC storage age on mortality.
Materials and Methods Five databases were searched through December 2014 for studies comparing mortality using transfused RBCs having longer and shorter storage times.
Results Analysis of six RCTs found no significant differences in survival comparing current practice (average storage age of 2 to 3 weeks) to transfusion of 1- to 10-day-old RBCs (OR 0.91, 95% CI 0.77-1.07). RBC storage age was lower in RCTs vs. observational studies (P = 0.01). The 31 observational studies found an increased risk of death (OR 1.13, 95% CI 1.03-1.24) (P = 0.01) with increasing age of RBCs, a different mortality effect than RCTs (P = 0.02).
Conclusion RCTs established that transfusion of 1- to 10-day-old stored RBCs is not superior to current practice. The apparent discrepancy in mortality between analyses of RCTs and observational studies may in part relate to differences in hypotheses tested and ages of stored RBCs studied. Further trials investigating 1- to 10-day-old stored RBC benefits would seem of lower priority than studies to determine whether 4- to 6-week stored units have safety and efficacy equivalent to the 2- to 3-week-old stored RBCs commonly transfused today.
C1 [Remy, K. E.; Sun, J.; Wang, D.; Solomon, S. B.; Natanson, C.; Cortes-Puch, I.] NIH, Dept Crit Care Med, Ctr Clin, 10 Ctr Dr,Room 2C145, Bethesda, MD 20892 USA.
[Welsh, J.] NIH, NIH Lib, Bldg 10, Bethesda, MD 20892 USA.
[Klein, H. G.] NIH, Dept Transfus Med, Ctr Clin, Bethesda, MD 20892 USA.
RP Remy, KE (reprint author), NIH, Dept Crit Care Med, Ctr Clin, 10 Ctr Dr,Room 2C145, Bethesda, MD 20892 USA.
EM Kenneth.remy@nih.gov; irene.cortespuch@nih.gov
FU NIH
FX Intramural sources at NIH.
NR 58
TC 8
Z9 8
U1 3
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0042-9007
EI 1423-0410
J9 VOX SANG
JI Vox Sang.
PD JUL
PY 2016
VL 111
IS 1
BP 43
EP 54
DI 10.1111/vox.12380
PG 12
WC Hematology
SC Hematology
GA DV2SY
UT WOS:000382772900006
PM 26848822
ER
PT J
AU Di Siena, S
Gimmelli, R
Nori, SL
Barbagallo, F
Campolo, F
Dolci, S
Rossi, P
Venneri, MA
Giannetta, E
Gianfrilli, D
Feigenbaum, L
Lenzi, A
Naro, F
Cianflone, E
Mancuso, T
Torella, D
Isidori, AM
Pellegrini, M
AF Di Siena, S.
Gimmelli, R.
Nori, S. L.
Barbagallo, F.
Campolo, F.
Dolci, S.
Rossi, P.
Venneri, M. A.
Giannetta, E.
Gianfrilli, D.
Feigenbaum, L.
Lenzi, A.
Naro, F.
Cianflone, E.
Mancuso, T.
Torella, D.
Isidori, A. M.
Pellegrini, M.
TI Activated c-Kit receptor in the heart promotes cardiac repair and
regeneration after injury
SO CELL DEATH & DISEASE
LA English
DT Article
ID STEM-CELL FACTOR; RANDOMIZED PHASE-1 TRIAL; MYOCARDIAL-INFARCTION;
C-KIT(+) CELLS; ISCHEMIC CARDIOMYOPATHY; IN-VIVO; W-LOCUS; MUTATIONS;
EXPRESSION; KINASE
AB The role of endogenous c-Kit receptor activation on cardiac cell homeostasis and repair remains largely unexplored. Transgenic mice carrying an activating point mutation (TgD814Y) in the kinase domain of the c-Kit gene were generated. c-Kit(TgD814Y) receptor was expressed in the heart during embryonic development and postnatal life, in a similar timing and expression pattern to that of the endogenous gene, but not in the hematopoietic compartment allowing the study of a cardiac-specific phenotype. c-Kit(TgD814Y) mutation produced a constitutive active c-Kit receptor in cardiac tissue and cells from transgenic mice as demonstrated by the increased phosphorylation of ERK1/2 and AKT, which are the main downstream molecular effectors of c-Kit receptor signaling. In adult transgenic hearts, cardiac morphology, size and total c-Kit(+) cardiac cell number was not different compared with wt mice. However, when c-Kit(TgD814Y) mice were subjected to transmural necrotic heart damage by cryoinjury (CI), all transgenic survived, compared with half of wt mice. In the sub-acute phase after CI, transgenic and wt mice showed similar heart damage. However, 9 days after CI, transgenic mice exhibited an increased number of c-Kit(+)CD31(+) endothelial progenitor cells surrounding the necrotic area. At later follow-up, a consistent reduction of fibrotic area, increased capillary density and increased cardiomyocyte replenishment rate (as established by BrdU incorporation) were observed in transgenic compared with wt mice. Consistently, CD45(-)c-Kit(+) cardiac stem cells isolated from transgenic c-Kit(TgD814Y) mice showed an enhanced endothelial and cardiomyocyte differentiation potential compared with cells isolated from the wt. Constitutive activation of c-Kit receptor in mice is associated with an increased cardiac myogenic and vasculogenic reparative potential after injury, with a significant improvement of survival.
C1 [Di Siena, S.; Naro, F.] Sapienza Univ, Dept Anat Histol Forens & Orthoped Sci, Rome, Italy.
[Gimmelli, R.; Barbagallo, F.; Campolo, F.; Venneri, M. A.; Giannetta, E.; Gianfrilli, D.; Lenzi, A.; Isidori, A. M.] Sapienza Univ, Dept Expt Med, Viale Policlin 155, I-00161 Rome, Italy.
[Nori, S. L.] Univ Salerno, Dept Med & Surg, Baronissi, Italy.
[Dolci, S.; Rossi, P.] Tor Vergata Univ, Dept Biomed & Prevent, Rome, Italy.
[Feigenbaum, L.] Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Frederick, MD USA.
[Cianflone, E.; Mancuso, T.; Torella, D.] Magna Graecia Univ Catanzaro, Dept Med & Surg Sci, Catanzaro, Italy.
[Pellegrini, M.] CNR, Inst Cell Biol & Neurobiol, Via E Ramarini 32, I-00015 Rome, Italy.
RP Isidori, AM (reprint author), Sapienza Univ, Dept Expt Med, Viale Policlin 155, I-00161 Rome, Italy.; Pellegrini, M (reprint author), CNR, Inst Cell Biol & Neurobiol, Via E Ramarini 32, I-00015 Rome, Italy.
EM andrea.isidori@uniroma1.it; manuela.pellegrini@cnr.it
FU PRIN; FIRB; MFG PRIN
FX PRIN 2010 to FN; FIRB 2010 to FN, AMI, AL; PRIN 2008 to SD; MFG 2009
PRIN 2012 to MP.
NR 52
TC 0
Z9 0
U1 6
U2 6
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 2041-4889
J9 CELL DEATH DIS
JI Cell Death Dis.
PD JUL
PY 2016
VL 7
AR e2317
DI 10.1038/cddis.2016.205
PG 15
WC Cell Biology
SC Cell Biology
GA DS6VD
UT WOS:000380920200003
PM 27468693
ER
PT J
AU Coleman, CN
Higgins, GS
Brown, JM
Baumann, M
Kirsch, DG
Willers, H
Prasanna, PGS
Dewhirst, MW
Bernhard, EJ
Ahmed, MM
AF Coleman, C. Norman
Higgins, Geoff S.
Brown, J. Martin
Baumann, Michael
Kirsch, David G.
Willers, Henning
Prasanna, Pataje G. S.
Dewhirst, Mark W.
Bernhard, Eric J.
Ahmed, Mansoor M.
TI Improving the Predictive Value of Preclinical Studies in Support of
Radiotherapy Clinical Trials
SO CLINICAL CANCER RESEARCH
LA English
DT Review
ID CANCER STEM-CELLS; RADIATION ONCOLOGY; TUMOR XENOGRAFTS; ANTICANCER
AGENTS; DRUG SCREEN; THERAPY; HETEROGENEITY; EXPRESSION;
RADIOSENSITIZERS; COMBINATIONS
AB There is an urgent need to improve reproducibility and translatability of preclinical data to fully exploit opportunities for molecular therapeutics involving radiation and radiochemotherapy. For in vitro research, the clonogenic assay remains the current state-of-the-art of preclinical assays, whereas newer moderate and high-throughput assays offer the potential for rapid initial screening. Studies of radiation response modification by molecularly targeted agents can be improved using more physiologic 3D culture models. Elucidating effects on the cancer stem cells (CSC, and CSC-like) and developing biomarkers for defining targets and measuring responses are also important. In vivo studies are necessary to confirm in vitro findings, further define mechanism of action, and address immunomodulation and treatment-induced modification of the microenvironment. Newer in vivo models include genetically engineered and patient-derived xenograft mouse models and spontaneously occurring cancers in domesticated animals. Selection of appropriate endpoints is important for in vivo studies; for example, regrowth delay measures bulk tumor killing, whereas local tumor control assesses effects on CSCs. The reliability of individual assays requires standardization of procedures and cross-laboratory validation. Radiation modifiers must be tested as part of clinical standard of care, which includes radiochemotherapy for most tumors. Radiation models are compatible with but also differ from those used for drug screening. Furthermore, the mechanism of a drug as a chemotherapeutic agent may be different from its interaction with radiation and/or radiochemotherapy. This provides an opportunity to expand the use of molecular-targeted agents. (C) 2016 AACR.
C1 [Coleman, C. Norman; Prasanna, Pataje G. S.; Bernhard, Eric J.; Ahmed, Mansoor M.] NCI, Radiat Res Program, Div Canc Treatment & Diag, NIH, 9609 Med Ctr Dr,3W102, Bethesda, MD 20892 USA.
[Higgins, Geoff S.] Univ Oxford, Oxford Inst Radiat Oncol, MRC, Canc Res UK, Oxford OX1 2JD, England.
[Brown, J. Martin] Stanford Univ, Sch Med, Dept Radiat Oncol, Stanford, CA 94305 USA.
[Baumann, Michael] Tech Univ Dresden, Helmholtz Zenrtum Dresden Rossendorf, OncoRay Natl Ctr Radiat Res, Dresden, Germany.
[Baumann, Michael] Dresden German Canc Res Ctr DKFZ, German Canc Consortium, Dresden, Germany.
[Kirsch, David G.; Dewhirst, Mark W.] Duke Univ, Dept Radiat Oncol, Durham, NC USA.
[Kirsch, David G.] Duke Univ, Dept Pharmacol, Durham, NC USA.
[Kirsch, David G.] Duke Univ, Dept Canc Biol, Durham, NC USA.
[Willers, Henning] Harvard Med Sch, Massachusetts Gen Hosp, Dept Radiat Oncol, Boston, MA USA.
[Dewhirst, Mark W.] Duke Univ, Dept Pathol, Durham, NC 27706 USA.
[Dewhirst, Mark W.] Duke Univ, Dept Biomed Engn, Durham, NC 27706 USA.
RP Coleman, CN (reprint author), NCI, Radiat Res Program, Div Canc Treatment & Diag, NIH, 9609 Med Ctr Dr,3W102, Bethesda, MD 20892 USA.
EM ccoleman@mail.nih.gov
FU American Cancer Society [123420RSG-12-224-01-DMC]
FX H. Willers is supported by the American Cancer Society
(123420RSG-12-224-01-DMC).
NR 71
TC 4
Z9 4
U1 5
U2 6
PU AMER ASSOC CANCER RESEARCH
PI PHILADELPHIA
PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA
SN 1078-0432
EI 1557-3265
J9 CLIN CANCER RES
JI Clin. Cancer Res.
PD JUL 1
PY 2016
VL 22
IS 13
BP 3138
EP 3147
DI 10.1158/1078-0432.CCR-16-0069
PG 10
WC Oncology
SC Oncology
GA DS7AE
UT WOS:000380933900005
PM 27154913
ER
PT J
AU Medvar, B
Raghuram, V
Pisitkun, T
Sarkar, A
Knepper, MA
AF Medvar, Barbara
Raghuram, Viswanathan
Pisitkun, Trairak
Sarkar, Abhijit
Knepper, Mark A.
TI Comprehensive database of human E3 ubiquitin ligases: application to
aquaporin-2 regulation
SO PHYSIOLOGICAL GENOMICS
LA English
DT Article
DE aquaporin-2; E3 ubiquitin ligases; collecting duct; kidney
ID MEDULLARY COLLECTING DUCT; LC-MS/MS; VASOPRESSIN; KIDNEY; RAT; CELLS;
PERMEABILITY; TRAFFICKING; PROTEINS; CHANNEL
AB Aquaporin-2 (AQP2) is regulated in part via vasopressin-mediated changes in protein half-life that are in turn dependent on AQP2 ubiquitination. Here we addressed the question, "What E3 ubiquitin ligase is most likely to be responsible for AQP2 ubiquitination?" using large-scale data integration based on Bayes' rule. The first step was to bioinformatically identify all E3 ligase genes coded by the human genome. The 377 E3 ubiquitin ligases identified in the human genome, consisting predominant of HECT, RING, and U-box proteins, have been used to create a publically accessible and downloadable online database (https://hpcwebapps.cit.nih.gov/ESBL/Database/E3-ligases/). We also curated a second database of E3 ligase accessory proteins that included BTB domain proteins, cullins, SOCS-box proteins, and F-box proteins. Using Bayes' theorem to integrate information from multiple large-scale proteomic and transcriptomic datasets, we ranked these 377 E3 ligases with respect to their probability of interaction with AQP2. Application of Bayes' rule identified the E3 ligases most likely to interact with AQP2 as (in order of probability): NEDD4 and NEDD4L (tied for first), AMFR, STUB1, ITCH, ZFPL1. Significantly, the two E3 ligases tied for top rank have also been studied extensively in the reductionist literature as regulatory proteins in renal tubule epithelia. The concordance of conclusions from reductionist and systems-level data provides strong motivation for further studies of the roles of NEDD4 and NEDD4L in the regulation of AQP2 protein turnover.
C1 [Medvar, Barbara; Raghuram, Viswanathan; Pisitkun, Trairak; Knepper, Mark A.] NHLBI, Epithelial Syst Biol Lab, NIH, Bethesda, MD USA.
[Medvar, Barbara; Sarkar, Abhijit] Catholic Univ Amer, Vitreous State Lab, Washington, DC USA.
[Pisitkun, Trairak] Chulalongkorn Univ, Fac Med, Syst Biol Ctr, Bangkok, Thailand.
RP Knepper, MA (reprint author), NIH, Bldg 10,Rm 6N307,10 Ctr Dr,MSC-1603, Bethesda, MD 20892 USA.
EM knepperm@nhlbi.nih.gov
FU Chulalongkorn Academic Advancement into Its 2nd Century (CUAASC)
Project; [HL-001285]; [HL-006129]
FX The study was carried out in the Division of Intramural Research of the
National Heart, Lung, and Blood Institute (Projects HL-001285 and
HL-006129, M. A. Knepper). T. Pisitkun is supported by the Chulalongkorn
Academic Advancement into Its 2nd Century (CUAASC) Project.
NR 32
TC 3
Z9 3
U1 1
U2 2
PU AMER PHYSIOLOGICAL SOC
PI BETHESDA
PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA
SN 1094-8341
EI 1531-2267
J9 PHYSIOL GENOMICS
JI Physiol. Genomics
PD JUL 1
PY 2016
VL 48
IS 7
BP 502
EP 512
DI 10.1152/physiolgenomics.00031.2016
PG 11
WC Cell Biology; Genetics & Heredity; Physiology
SC Cell Biology; Genetics & Heredity; Physiology
GA DS7ZA
UT WOS:000381000800007
PM 27199454
ER
PT J
AU Carlin, DJ
Naujokas, MF
Bradham, KD
Cowden, J
Heacock, M
Henry, HF
Lee, JS
Thomas, DJ
Thompson, C
Tokar, EJ
Waalkes, MP
Birnbaum, LS
Suk, WA
AF Carlin, Danielle J.
Naujokas, Marisa F.
Bradham, Karen D.
Cowden, John
Heacock, Michelle
Henry, Heather F.
Lee, Janice S.
Thomas, David J.
Thompson, Claudia
Tokar, Erik J.
Waalkes, Michael P.
Birnbaum, Linda S.
Suk, William A.
TI Arsenic and Environmental Health: State of the Science and Future
Research Opportunities
SO ENVIRONMENTAL HEALTH PERSPECTIVES
LA English
DT Review
ID DIFFERENTIAL DNA METHYLATION; UMBILICAL-CORD BLOOD; FATTY LIVER-DISEASE;
DRINKING-WATER; RISK-ASSESSMENT; IN-UTERO; GUT MICROBIOME; RELATIVE
BIOAVAILABILITY; REMEDIATION TECHNIQUES; 21ST-CENTURY ROADMAP
AB BACKGROUND: Exposure to inorganic and organic arsenic compounds is a major public health problem that affects hundreds of millions of people worldwide. Exposure to arsenic is associated with cancer and noncancer effects in nearly every organ in the body, and evidence is mounting for health effects at lower levels of arsenic exposure than previously thought. Building from a tremendous knowledge base with > 1,000 scientific papers published annually with "arsenic" in the title, the question becomes, what questions would best drive future research directions?
OBJECTIVES: The objective is to discuss emerging issues in arsenic research and identify data gaps across disciplines.
METHODS: The National Institutes of Health's National Institute of Environmental Health Sciences Superfund Research Program convened a workshop to identify emerging issues and research needs to address the multi-faceted challenges related to arsenic and environmental health. This review summarizes information captured during the workshop.
DISCUSSION: More information about aggregate exposure to arsenic is needed, including the amount and forms of arsenic found in foods. New strategies for mitigating arsenic exposures and related health effects range from engineered filtering systems to phytogenetics and nutritional interventions. Furthermore, integration of omics data with mechanistic and epidemiological data is a key step toward the goal of linking biomarkers of exposure and susceptibility to disease mechanisms and outcomes.
Conclusions: Promising research strategies and technologies for arsenic exposure and adverse health effect mitigation are being pursued, and future research is moving toward deeper collaborations and integration of information across disciplines to address data gaps.
C1 [Carlin, Danielle J.; Heacock, Michelle; Henry, Heather F.; Suk, William A.] NIEHS, Superfund Res Program, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA.
[Naujokas, Marisa F.] MDB Inc, Durham, NC USA.
[Bradham, Karen D.] US EPA, Human Exposure & Atmospher Sci Div, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA.
[Cowden, John] US EPA, Natl Ctr Computat Toxicol, ORD, Res Triangle Pk, NC 27711 USA.
[Lee, Janice S.] US EPA, Natl Ctr Environm Assessment, ORD, Res Triangle Pk, NC 27711 USA.
[Thomas, David J.] US EPA, Integrated Syst Toxicol Div, Natl Human & Environm Hlth Effects Res Lab, ORD, Res Triangle Pk, NC 27711 USA.
[Thompson, Claudia] NIEHS, Populat Hlth Branch, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA.
[Tokar, Erik J.; Waalkes, Michael P.; Birnbaum, Linda S.] NIEHS, Natl Toxicol Program, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA.
[Birnbaum, Linda S.] NIEHS, NIH, US Dept HHS, Res Triangle Pk, NC 27560 USA.
RP Carlin, DJ (reprint author), NIEHS, Div Extramural Res & Training, Hazardous Subst Res Branch, Superfund Res Program, Keystone Bldg,530 Davis Dr,POB 12233, Res Triangle Pk, NC 27560 USA.
EM danielle.carlin@nih.gov
FU NIH, NIEHS [ES-102925]
FX This work was supported in part by the NIH, NIEHS, including grant
ES-102925.
NR 130
TC 6
Z9 6
U1 30
U2 41
PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE
PI RES TRIANGLE PK
PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233,
RES TRIANGLE PK, NC 27709-2233 USA
SN 0091-6765
EI 1552-9924
J9 ENVIRON HEALTH PERSP
JI Environ. Health Perspect.
PD JUL
PY 2016
VL 124
IS 7
BP 890
EP 899
DI 10.1289/ehp.1510209
PG 10
WC Environmental Sciences; Public, Environmental & Occupational Health;
Toxicology
SC Environmental Sciences & Ecology; Public, Environmental & Occupational
Health; Toxicology
GA DS4KF
UT WOS:000380749300009
PM 26587579
ER
PT J
AU Mansouri, K
Abdelaziz, A
Rybacka, A
Roncaglioni, A
Tropsha, A
Varnek, A
Zakharov, A
Worth, A
Richard, AM
Grulke, CM
Trisciuzzi, D
Fourches, D
Horvath, D
Benfenati, E
Muratov, E
Wedebye, EB
Grisoni, F
Mangiatordi, GF
Incisivo, GM
Hong, HX
Ng, HW
Tetko, IV
Balabin, I
Kancherla, J
Shen, J
Burton, J
Nicklaus, M
Cassotti, M
Nikolov, NG
Nicolotti, O
Andersson, PL
Zang, QD
Politi, R
Beger, RD
Todeschini, R
Huang, RL
Farag, S
Rosenberg, SA
Slavov, S
Hu, X
Judson, RS
AF Mansouri, Kamel
Abdelaziz, Ahmed
Rybacka, Aleksandra
Roncaglioni, Alessandra
Tropsha, Alexander
Varnek, Alexandre
Zakharov, Alexey
Worth, Andrew
Richard, Ann M.
Grulke, Christopher M.
Trisciuzzi, Daniela
Fourches, Denis
Horvath, Dragos
Benfenati, Emilio
Muratov, Eugene
Wedebye, Eva Bay
Grisoni, Francesca
Mangiatordi, Giuseppe F.
Incisivo, Giuseppina M.
Hong, Huixiao
Ng, Hui W.
Tetko, Igor V.
Balabin, Ilya
Kancherla, Jayaram
Shen, Jie
Burton, Julien
Nicklaus, Marc
Cassotti, Matteo
Nikolov, Nikolai G.
Nicolotti, Orazio
Andersson, Patrik L.
Zang, Qingda
Politi, Regina
Beger, Richard D.
Todeschini, Roberto
Huang, Ruili
Farag, Sherif
Rosenberg, Sine A.
Slavov, Svetoslav
Hu, Xin
Judson, Richard S.
TI CERAPP: Collaborative Estrogen Receptor Activity Prediction Project
SO ENVIRONMENTAL HEALTH PERSPECTIVES
LA English
DT Article
ID ENDOCRINE-DISRUPTING CHEMICALS; COMPUTATIONAL TOXICOLOGY RESOURCE;
QUANTITATIVE STRUCTURE-ACTIVITY; THROUGHPUT SCREENING ASSAYS;
RELATIONSHIP QSDAR MODELS; ENVIRONMENTAL CHEMICALS; DECISION FOREST;
PARTITION-COEFFICIENTS; BINARY CLASSIFICATION; BIOLOGICAL-ACTIVITY
AB BACKGROUND: Humans are exposed to thousands of man-made chemicals in the environment. Some chemicals mimic natural endocrine hormones and, thus, have the potential to be endocrine disruptors. Most of these chemicals have never been tested for their ability to interact with the estrogen receptor (ER). Risk assessors need tools to prioritize chemicals for evaluation in costly in vivo tests, for instance, within the U.S. EPA Endocrine Disruptor Screening Program.
OBJECTIVES: We describe a large-scale modeling project called CERAPP (Collaborative Estrogen Receptor Activity Prediction Project) and demonstrate the efficacy of using predictive computational models trained on high-throughput screening data to evaluate thousands of chemicals for ER-related activity and prioritize them for further testing.
METHODS: CERAPP combined multiple models developed in collaboration with 17 groups in the United States and Europe to predict ER activity of a common set of 32,464 chemical structures. Quantitative structure-activity relationship models and docking approaches were employed, mostly using a common training set of 1,677 chemical structures provided by the U.S. EPA, to build a total of 40 categorical and 8 continuous models for binding, agonist, and antagonist ER activity. All predictions were evaluated on a set of 7,522 chemicals curated from the literature. To overcome the limitations of single models, a consensus was built by weighting models on scores based on their evaluated accuracies.
RESULTS: Individual model scores ranged from 0.69 to 0.85, showing high prediction reliabilities. Out of the 32,464 chemicals, the consensus model predicted 4,001 chemicals (12.3%) as high priority actives and 6,742 potential actives (20.8%) to be considered for further testing.
CONCLUSION: This project demonstrated the possibility to screen large libraries of chemicals using a consensus of different in silico approaches. This concept will be applied in future projects related to other end points.
C1 [Mansouri, Kamel; Richard, Ann M.; Grulke, Christopher M.; Kancherla, Jayaram; Judson, Richard S.] US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA.
[Mansouri, Kamel] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA.
[Abdelaziz, Ahmed; Tetko, Igor V.] German Res Ctr Environm Hlth GmbH, Helmholtz Zentrum Muenchen, Inst Struct Biol, Neuherberg, Germany.
[Rybacka, Aleksandra; Andersson, Patrik L.] Umea Univ, Dept Chem, Umea, Sweden.
[Roncaglioni, Alessandra; Benfenati, Emilio; Incisivo, Giuseppina M.] Ist Ric Farmacol Mario Negri, IRCCS, Environm Chem & Toxicol Lab, Milan, Italy.
[Tropsha, Alexander; Fourches, Denis; Muratov, Eugene; Politi, Regina; Farag, Sherif] Univ North Carolina Chapel Hill, Lab Mol Modeling, Chapel Hill, NC USA.
[Varnek, Alexandre; Horvath, Dragos] Univ Strasbourg, Lab Chemoinformat, Strasbourg, France.
[Zakharov, Alexey; Nicklaus, Marc] NCI, NIH, US Dept HHS, Bethesda, MD 20892 USA.
[Worth, Andrew; Burton, Julien] European Commiss Ispra, Joint Res Ctr, IHCP, Ispra, Italy.
[Trisciuzzi, Daniela; Mangiatordi, Giuseppe F.; Nicolotti, Orazio] Univ Bari, Dept Pharmacy Drug Sci, Bari, Italy.
[Wedebye, Eva Bay; Nikolov, Nikolai G.; Rosenberg, Sine A.] Tech Univ Denmark, Natl Food Inst, Div Toxicol & Risk Assessment, Copenhagen, Denmark.
[Grisoni, Francesca; Cassotti, Matteo; Todeschini, Roberto] Univ Milano Bicocca, Milano Chemometr & QSAR Res Grp, Milan, Italy.
[Hong, Huixiao; Ng, Hui W.] US FDA, Div Bioinformat & Biostat, Natl Ctr Toxicol Res, USDA, Jefferson, AZ USA.
[Tetko, Igor V.] BigChem GmbH, Neuherberg, Germany.
[Balabin, Ilya] Lockheed Martin, High Performance Comp, Res Triangle Pk, NC USA.
[Shen, Jie] Res Inst Fragrance Mat Inc, Woodcliff Lake, NJ USA.
[Zang, Qingda; Slavov, Svetoslav] Integrated Lab Syst Inc, Res Triangle Pk, NC USA.
[Beger, Richard D.] USDA, Div Syst Biol, Natl Ctr Toxicol Res, Jefferson, AZ USA.
[Huang, Ruili; Hu, Xin] NIH, Natl Ctr Adv Translat Sci, DHHS, Bldg 10, Bethesda, MD 20892 USA.
RP Judson, RS (reprint author), US EPA, Natl Ctr Computat Toxicol, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA.
EM judson.richard@epa.gov
RI Tetko, Igor/B-1540-2010; Varnek, Alexandre/E-7076-2017; Kancherla,
Jayaram/P-7756-2015;
OI Tetko, Igor/0000-0002-6855-0012; Varnek, Alexandre/0000-0003-1886-925X;
Kancherla, Jayaram/0000-0001-5855-5031; Mansouri,
Kamel/0000-0002-6426-8036
FU NIGMS NIH HHS [T32 GM067553]
NR 101
TC 17
Z9 17
U1 13
U2 15
PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE
PI RES TRIANGLE PK
PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233,
RES TRIANGLE PK, NC 27709-2233 USA
SN 0091-6765
EI 1552-9924
J9 ENVIRON HEALTH PERSP
JI Environ. Health Perspect.
PD JUL
PY 2016
VL 124
IS 7
BP 1023
EP 1033
DI 10.1289/ehp.1510267
PG 11
WC Environmental Sciences; Public, Environmental & Occupational Health;
Toxicology
SC Environmental Sciences & Ecology; Public, Environmental & Occupational
Health; Toxicology
GA DS4KF
UT WOS:000380749300025
PM 26908244
ER
PT J
AU Leung, MCK
Phuong, J
Baker, NC
Sipes, NS
Klinefelter, GR
Martin, MT
McLaurin, KW
Setzer, RW
Darney, SP
Judson, RS
Knudsen, TB
AF Leung, Maxwell C. K.
Phuong, Jimmy
Baker, Nancy C.
Sipes, Nisha S.
Klinefelter, Gary R.
Martin, Matthew T.
McLaurin, Keith W.
Setzer, R. Woodrow
Darney, Sally Perreault
Judson, Richard S.
Knudsen, Thomas B.
TI Systems Toxicology of Male Reproductive Development: Profiling 774
Chemicals for Molecular Targets and Adverse Outcomes
SO ENVIRONMENTAL HEALTH PERSPECTIVES
LA English
DT Article
ID TESTICULAR DYSGENESIS SYNDROME; INTERLEUKIN-6 SIGNAL BLOCKADE; GERM-CELL
DIFFERENTIATION; RECEPTOR TYROSINE KINASE; GENOME-WIDE ASSOCIATION;
LARGE GENE LISTS; FETAL TESTIS; DI(N-BUTYL) PHTHALATE; NUCLEAR
RECEPTORS; DIETHYLHEXYL PHTHALATE
AB BACKGROUND: Trends in male reproductive health have been reported for increased rates of testicular germ cell tumors, low semen quality, cryptorchidism, and hypospadias, which have been associated with prenatal environmental chemical exposure based on human and animal studies.
OBJECTIVE: In the present study we aimed to identify significant correlations between environmental chemicals, molecular targets, and adverse outcomes across a broad chemical landscape with emphasis on developmental toxicity of the male reproductive system.
METHODS: We used U.S. EPA's animal study database (ToxRefDB) and a comprehensive literature analysis to identify 774 chemicals that have been evaluated for adverse effects on male reproductive parameters, and then used U.S. EPA's in vitro high-throughput screening (HTS) database (ToxCastDB) to profile their bioactivity across approximately 800 molecular and cellular features.
RESULTS: A phenotypic hierarchy of testicular atrophy, sperm effects, tumors, and malformations, a composite resembling the human testicular dysgenesis syndrome (TDS) hypothesis, was observed in 281 chemicals. A subset of 54 chemicals with male developmental consequences had in vitro bioactivity on molecular targets that could be condensed into 156 gene annotations in a bipartite network.
CONCLUSION: Computational modeling of available in vivo and in vitro data for chemicals that produce adverse effects on male reproductive end points revealed a phenotypic hierarchy across animal studies consistent with the human TDS hypothesis. We confirmed the known role of estrogen and androgen signaling pathways in rodent TDS, and importantly, broadened the list of molecular targets to include retinoic acid signaling, vascular remodeling proteins, G-protein coupled receptors (GPCRs), and cytochrome P450s.
C1 [Leung, Maxwell C. K.; Sipes, Nisha S.; McLaurin, Keith W.] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA.
[Leung, Maxwell C. K.; Phuong, Jimmy; Sipes, Nisha S.; Martin, Matthew T.; McLaurin, Keith W.; Setzer, R. Woodrow; Judson, Richard S.; Knudsen, Thomas B.] US EPA, Natl Ctr Computat Toxicol, Res Triangle Pk, NC 27711 USA.
[Baker, Nancy C.] Lockheed Martin, Res Triangle Pk, NC USA.
[Klinefelter, Gary R.; Darney, Sally Perreault] US EPA, Natl Hlth & Environm Effects Res Lab, Res Triangle Pk, NC 27711 USA.
[Darney, Sally Perreault] NIEHS, Environm Hlth Perspect, NIH, US Dept HHS, POB 12233, Res Triangle Pk, NC 27709 USA.
RP Leung, MCK; Knudsen, TB (reprint author), US EPA, 109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA.
EM leung.max-well@epa.gov; knudsen.thomas@epa.gov
OI Leung, Maxwell/0000-0003-1530-3306
NR 98
TC 2
Z9 2
U1 8
U2 12
PU US DEPT HEALTH HUMAN SCIENCES PUBLIC HEALTH SCIENCE
PI RES TRIANGLE PK
PA NATL INST HEALTH, NATL INST ENVIRONMENTAL HEALTH SCIENCES, PO BOX 12233,
RES TRIANGLE PK, NC 27709-2233 USA
SN 0091-6765
EI 1552-9924
J9 ENVIRON HEALTH PERSP
JI Environ. Health Perspect.
PD JUL
PY 2016
VL 124
IS 7
BP 1050
EP 1061
DI 10.1289/ehp.1510385
PG 12
WC Environmental Sciences; Public, Environmental & Occupational Health;
Toxicology
SC Environmental Sciences & Ecology; Public, Environmental & Occupational
Health; Toxicology
GA DS4KF
UT WOS:000380749300028
PM 26662846
ER
PT J
AU Larson, CL
Martinez, E
Beare, PA
Jeffrey, B
Heinzen, RA
Bonazzi, M
AF Larson, Charles L.
Martinez, Eric
Beare, Paul A.
Jeffrey, Brendan
Heinzen, Robert A.
Bonazzi, Matteo
TI Right on Q: genetics begin to unravel Coxiella burnetii host cell
interactions
SO FUTURE MICROBIOLOGY
LA English
DT Review
DE apoptosis; autophagy; Coxiella burnetii; effector protein; host cell
invasion; macrophages; mutagenesis; phagolysosome; type 4B secretion;
vacuole remodeling
ID IV SECRETION SYSTEM; CLATHRIN-MEDIATED ENDOCYTOSIS; PNEUMOPHILA DOT/ICM
SYSTEM; RESPONSE REGULATOR PMRA; Q-FEVER; LEGIONELLA-PNEUMOPHILA;
PHASE-II; EFFECTOR PROTEIN; INTRACELLULAR REPLICATION; ANTIAPOPTOTIC
ACTIVITY
AB Invasion of macrophages and replication within an acidic and degradative phagolysosome-like vacuole are essential for disease pathogenesis by Coxiella burnetii, the bacterial agent of human Q fever. Previous experimental constraints imposed by the obligate intracellular nature of Coxiella limited knowledge of pathogen strategies that promote infection. Fortunately, new genetic tools facilitated by axenic culture now allow allelic exchange and transposon mutagenesis approaches for virulence gene discovery. Phenotypic screens have illuminated the critical importance of Coxiella's type 4B secretion system in host cell subversion and discovered genes encoding translocated effector proteins that manipulate critical infection events. Here, we highlight the cellular microbiology and genetics of Coxiella and how recent technical advances now make Coxiella a model organism to study macrophage parasitism.
C1 [Larson, Charles L.; Beare, Paul A.; Heinzen, Robert A.] NIAID, Coxiella Pathogenesis Sect, Bacteriol Lab, Rocky Mt Labs,NIH, 903 South 4th St, Hamilton, MT 59840 USA.
[Martinez, Eric; Bonazzi, Matteo] CNRS, FRE3698, CPBS, 1919 Route Mende, F-34293 Montpellier, France.
[Martinez, Eric; Bonazzi, Matteo] Univ Montpellier, Montpellier, France.
[Jeffrey, Brendan] NIAID, Bioinformat & Computat Biosci Branch, Rocky Mt Labs, NIH, 903 South 4th St, Hamilton, MT 59840 USA.
RP Heinzen, RA (reprint author), NIAID, Coxiella Pathogenesis Sect, Bacteriol Lab, Rocky Mt Labs,NIH, 903 South 4th St, Hamilton, MT 59840 USA.
EM rheinzen@niaid.nih.gov
FU Intramural Research Program of the NIH, National Institute of Allergy
and Infectious Diseases; Agence Nationale de la Recherche (ANR)
[ANR-14-CE14-0012-01]; ERA-NET Infect-ERA [ANR-13-IFEC-0003];
ATIP-AVENIR programme
FX This work was supported by the Intramural Research Program of the NIH,
National Institute of Allergy and Infectious Diseases (RA Heinzen) and
by grants from the Agence Nationale de la Recherche (ANR)
(ANR-14-CE14-0012-01; Project AttaQ), ERA-NET Infect-ERA
(ANR-13-IFEC-0003; Project EUGENPATH) and the ATIP-AVENIR programme (M
Bonazzi). The authors have no other relevant affiliations or financial
involvement with any organization or entity with a financial interest in
or financial conflict with the subject matter or materials discussed in
the manuscript apart from those disclosed.
NR 155
TC 2
Z9 2
U1 1
U2 3
PU FUTURE MEDICINE LTD
PI LONDON
PA UNITEC HOUSE, 3RD FLOOR, 2 ALBERT PLACE, FINCHLEY CENTRAL, LONDON, N3
1QB, ENGLAND
SN 1746-0913
EI 1746-0921
J9 FUTURE MICROBIOL
JI Future Microbiol.
PD JUL
PY 2016
VL 11
IS 7
BP 919
EP 939
DI 10.2217/fmb-2016-0044
PG 21
WC Microbiology
SC Microbiology
GA DS8LB
UT WOS:000381033700008
PM 27418426
ER
PT J
AU Alexander-Bloch, A
Clasen, L
Stockman, M
Ronan, L
Lalonde, F
Giedd, J
Raznahan, A
AF Alexander-Bloch, Aaron
Clasen, Liv
Stockman, Michael
Ronan, Lisa
Lalonde, Francois
Giedd, Jay
Raznahan, Armin
TI Subtle In-Scanner Motion Biases Automated Measurement of Brain Anatomy
From In Vivo MRI
SO HUMAN BRAIN MAPPING
LA English
DT Article
DE functional neuroimaging; magnetic resonance imaging; motion; bias;
cortical thickness; cortical surface area; cortical curvature
ID FMRI TIME-SERIES; HUMAN CEREBRAL-CORTEX; FUNCTIONAL CONNECTIVITY MRI;
MAGNETIC-RESONANCE IMAGES; SURFACE-BASED ANALYSIS; HEAD MOTION;
NAVIGATOR ECHOES; GEOMETRICALLY ACCURATE; ADAPTIVE TECHNIQUE; MOVING
STRUCTURES
AB While the potential for small amounts of motion in functional magnetic resonance imaging (fMRI) scans to bias the results of functional neuroimaging studies is well appreciated, the impact of in-scanner motion on morphological analysis of structural MRI is relatively under-studied. Even among "good quality" structural scans, there may be systematic effects of motion on measures of brain morphometry. In the present study, the subjects' tendency to move during fMRI scans, acquired in the same scanning sessions as their structural scans, yielded a reliable, continuous estimate of in-scanner motion. Using this approach within a sample of 127 children, adolescents, and young adults, significant relationships were found between this measure and estimates of cortical gray matter volume and mean curvature, as well as trend-level relationships with cortical thickness. Specifically, cortical volume and thickness decreased with greater motion, and mean curvature increased. These effects of subtle motion were anatomically heterogeneous, were present across different automated imaging pipelines, showed convergent validity with effects of frank motion assessed in a separate sample of 274 scans, and could be demonstrated in both pediatric and adult populations. Thus, using different motion assays in two large non-overlapping sets of structural MRI scans, convergent evidence showed that in-scanner motion-even at levels which do not manifest in visible motion artifact-can lead to systematic and regionally specific biases in anatomical estimation. These findings have special relevance to structural neuroimaging in developmental and clinical datasets, and inform ongoing efforts to optimize neuroanatomical analysis of existing and future structural MRI datasets in non-sedated humans. (C) 2016 Wiley Periodicals, Inc.
C1 [Alexander-Bloch, Aaron; Clasen, Liv; Stockman, Michael; Lalonde, Francois; Raznahan, Armin] NIMH, Dev Neurogen Unit, Child Psychiat Branch, 10 Ctr Dr,Bldg 10,Room 4D18, Bethesda, MD 20892 USA.
[Alexander-Bloch, Aaron] Yale Univ, Sch Med, Dept Psychiat, New Haven, CT 06508 USA.
[Ronan, Lisa] Univ Cambridge, Brain Mapping Unit, Cambridge, England.
[Giedd, Jay] Univ Calif San Diego, Dept Psychiat, San Diego, CA 92093 USA.
RP Raznahan, A (reprint author), NIMH, Dev Neurogen Unit, Child Psychiat Branch, 10 Ctr Dr,Bldg 10,Room 4D18, Bethesda, MD 20892 USA.
EM raznahana@mail.nih.gov
FU Intramural NIH HHS [Z99 MH999999]
NR 62
TC 5
Z9 5
U1 0
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1065-9471
EI 1097-0193
J9 HUM BRAIN MAPP
JI Hum. Brain Mapp.
PD JUL
PY 2016
VL 37
IS 7
BP 2385
EP 2397
DI 10.1002/hbm.23180
PG 13
WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical
Imaging
SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging
GA DU8TV
UT WOS:000382488800002
PM 27004471
ER
PT J
AU Smith, E
Thurm, A
Greenstein, D
Farmer, C
Swedo, S
Giedd, J
Raznahan, A
AF Smith, Elizabeth
Thurm, Audrey
Greenstein, Deanna
Farmer, Cristan
Swedo, Susan
Giedd, Jay
Raznahan, Armin
TI Cortical Thickness Change in Autism During Early Childhood
SO HUMAN BRAIN MAPPING
LA English
DT Article
DE autism spectrum disorder; cortical thickness; surface based morphometry;
expressive language; MRI
ID LANGUAGE-ASSOCIATION CORTEX; SPECTRUM DISORDERS; WHITE-MATTER;
GRAY-MATTER; LONGITUDINAL CHANGES; BRAIN MATURATION; YOUNG ADULTHOOD;
CEREBRAL-CORTEX; MOTOR CORTEX; SURFACE-AREA
AB Structural magnetic resonance imaging (MRI) scans at high spatial resolution can detect potential foci of early brain dysmaturation in children with autism spectrum disorders (ASD). In addition, comparison between MRI and behavior measures over time can identify patterns of brain change accompanying specific outcomes. We report structural MRI data from two time points for a total of 84 scans in children with ASD and 30 scans in typical controls (mean age time one = 4.1 years, mean age at time two - 6.6 years). Surface-based cortical morphometry and linear mixed effects models were used to link changes in cortical anatomy to both diagnostic status and individual differences in changes in language and autism severity. Compared with controls, children with ASD showed accelerated gray matter volume gain with age, which was driven by a lack of typical age-related cortical thickness (CT) decrease within 10 cortical regions involved in language, social cognition, and behavioral control. Greater expressive communication gains with age in children with ASD were associated with greater CT gains in a set of right hemisphere homologues to dominant language cortices, potentially identifying a compensatory system for closer translational study. (C) 2016 Wiley Periodicals, Inc.
C1 [Smith, Elizabeth; Thurm, Audrey; Farmer, Cristan; Swedo, Susan] NIMH, Pediat & Dev Neurosci Branch, 10 Ctr Dr MSC 1255,Bldg 10,Room 1C250, Bethesda, MD 20892 USA.
[Greenstein, Deanna; Raznahan, Armin] NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA.
[Giedd, Jay] Univ Calif San Diego, Dept Psychiat, San Diego, CA 92103 USA.
RP Smith, E (reprint author), NIMH, Pediat & Dev Neurosci Branch, 10 Ctr Dr MSC 1255,Bldg 10,Room 1C250, Bethesda, MD 20892 USA.
EM elizabeth.smith3@nih.gov
FU Intramural NIH HHS [ZIA MH002914-08]; NIMH NIH HHS [ZIA MH002914]
NR 87
TC 2
Z9 2
U1 19
U2 21
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1065-9471
EI 1097-0193
J9 HUM BRAIN MAPP
JI Hum. Brain Mapp.
PD JUL
PY 2016
VL 37
IS 7
BP 2616
EP 2629
DI 10.1002/hbm.23195
PG 14
WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical
Imaging
SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging
GA DU8TV
UT WOS:000382488800019
PM 27061356
ER
PT J
AU Merlino, G
Herlyn, M
Fisher, DE
Bastian, BC
Flaherty, KT
Davies, MA
Wargo, JA
Curiel-Lewandrowski, C
Weber, MJ
Leachman, SA
Soengas, MS
McMahon, M
Harbour, JW
Swetter, SM
Aplin, AE
Atkins, MB
Bosenberg, MW
Dummer, R
Gershenwald, JE
Halpern, AC
Herlyn, D
Karakousis, GC
Kirkwood, JM
Krauthammer, M
Lo, RS
Long, GV
McArthur, G
Ribas, A
Schuchter, L
Sosman, JA
Smalley, KS
Steeg, P
Thomas, NE
Tsao, H
Tueting, T
Weeraratna, A
Xu, G
Lomax, R
Martin, A
Silverstein, S
Turnham, T
Ronai, ZA
AF Merlino, Glenn
Herlyn, Meenhard
Fisher, David E.
Bastian, Boris C.
Flaherty, Keith T.
Davies, Michael A.
Wargo, Jennifer A.
Curiel-Lewandrowski, Clara
Weber, Michael J.
Leachman, Sancy A.
Soengas, Maria S.
McMahon, Martin
Harbour, J. William
Swetter, Susan M.
Aplin, Andrew E.
Atkins, Michael B.
Bosenberg, Marcus W.
Dummer, Reinhard
Gershenwald, Jeffrey E.
Halpern, Allan C.
Herlyn, Dorothee
Karakousis, Giorgos C.
Kirkwood, John M.
Krauthammer, Michael
Lo, Roger S.
Long, Georgina V.
McArthur, Grant
Ribas, Antoni
Schuchter, Lynn
Sosman, Jeffrey A.
Smalley, Keiran S.
Steeg, Patricia
Thomas, Nancy E.
Tsao, Hensin
Tueting, Thomas
Weeraratna, Ashani
Xu, George
Lomax, Randy
Martin, Alison
Silverstein, Steve
Turnham, Tim
Ronai, Ze'ev A.
TI The state of melanoma: challenges and opportunities
SO PIGMENT CELL & MELANOMA RESEARCH
LA English
DT Article
DE melanoma; prevention; early diagnosis; metastasis; dormancy; therapy
AB The Melanoma Research Foundation (MRF) has charted a comprehensive assessment of the current state of melanoma research and care. Intensive discussions among members of the MRF Scientific Advisory Council and Breakthrough Consortium, a group that included clinicians and scientists, focused on four thematic areas diagnosis/early detection, prevention, tumor cell dormancy (including metastasis), and therapy (response and resistance). These discussions extended over the course of 2015 and culminated at the Society of Melanoma Research 2015 International Congress in November. Each of the four groups has outlined their thoughts as per the current status, challenges, and opportunities in the four respective areas. The current state and immediate and longterm needs of the melanoma field, from basic research to clinical management, are presented in the following report.
C1 [Merlino, Glenn] NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA.
[Herlyn, Meenhard; Herlyn, Dorothee; Weeraratna, Ashani] Wistar Inst Anat & Biol, Melanoma Res Ctr, 3601 Spruce St, Philadelphia, PA 19104 USA.
[Fisher, David E.] Massachusetts Gen Hosp, Dept Dermatol, Cutaneous Biol Res Ctr, Charlestown, MA USA.
[Bastian, Boris C.] Univ Calif San Francisco, Dept Dermatol, Helen Diller Family Comprehens Canc Ctr, San Francisco, CA 94143 USA.
[Bastian, Boris C.] Univ Calif San Francisco, Dept Pathol, Helen Diller Family Comprehens Canc Ctr, San Francisco, CA 94140 USA.
[Flaherty, Keith T.] Massachusetts Gen Hosp, Dev Therapeut, Ctr Canc, Charlestown, MA USA.
[Davies, Michael A.; Wargo, Jennifer A.; Gershenwald, Jeffrey E.] Univ Texas MD Anderson Canc Ctr, Dept Genom Med & Surg Oncol, Houston, TX 77030 USA.
[Curiel-Lewandrowski, Clara] Univ Arizona, Ctr Canc, Dept Med, Tucson, AZ USA.
[Curiel-Lewandrowski, Clara] Univ Arizona, Ctr Canc, Dept Dermatol, Tucson, AZ USA.
[Weber, Michael J.] Univ Virginia, Sch Med, Dept Microbiol Immunol & Canc Biol, Charlottesville, VA 22908 USA.
[Leachman, Sancy A.] Oregon Hlth & Sci Univ, Dept Dermatol, Knight Canc Inst, Melanoma & Skin Canc Program, Portland, OR USA.
[Soengas, Maria S.] CNIO, Mol Oncol Program, Melanoma Lab, Madrid, Spain.
[McMahon, Martin] Huntsman Canc Inst, Dept Dermatol, Salt Lake City, UT USA.
[Harbour, J. William] Univ Miami, Bascom Palmer Eye Inst, Miami, FL USA.
[Harbour, J. William] Univ Miami, Sylvester Comprehens Canc Ctr, Miami, FL USA.
[Swetter, Susan M.] Stanford Univ, Med Ctr, Dept Dermatol, Palo Alto, CA 94304 USA.
[Swetter, Susan M.] VA Palo Alto Hlth Care Syst, Inst Canc, Palo Alto, CA USA.
[Aplin, Andrew E.] Thomas Jefferson Univ, Sidney Kimmel Canc Ctr, Philadelphia, PA 19107 USA.
[Atkins, Michael B.] Georgetown Univ, Lombardi Comprehens Canc Ctr, Washington, DC USA.
[Bosenberg, Marcus W.] Yale Univ, Ctr Canc, Dept Dermatol & Dermatopathol, New Haven, CT USA.
[Dummer, Reinhard] Univ Zurich, Dept Dermatol, Zurich, Switzerland.
[Halpern, Allan C.] Mem Sloan Kettering Canc Ctr, Dermatol, 1275 York Ave, New York, NY 10021 USA.
[Karakousis, Giorgos C.; Schuchter, Lynn; Xu, George] Univ Penn, Abramson Canc Ctr, Philadelphia, PA 19104 USA.
[Kirkwood, John M.] Univ Pittsburgh, Melanoma & Skin Canc Program, Pittsburgh, PA USA.
[Krauthammer, Michael] Yale Univ, Dept Pathol, Ctr Canc, New Haven, CT USA.
[Lo, Roger S.; Ribas, Antoni] Univ Calif Los Angeles, Dept Med Oncol & Dermatol, Los Angeles, CA USA.
[Long, Georgina V.] Univ Sydney, Melanoma Inst, Sydney, NSW, Australia.
[McArthur, Grant] Peter MacCallum Canc Ctr, Dept Canc Med, East Melbourne, Vic, Australia.
[Sosman, Jeffrey A.] Vanderbilt Univ, Sch Med, Vanderbilt Ingram Canc Ctr, Dept Med, Nashville, TN 37212 USA.
[Smalley, Keiran S.] H Lee Moffitt Canc Ctr & Res Inst, Dept Tumor Biol, Tampa, FL USA.
[Steeg, Patricia] NCI, Women Malignancies Branch, Ctr Canc Res, Bethesda, MD 20892 USA.
[Thomas, Nancy E.] Univ N Carolina, Melanoma Program, Dept Dermatol, Lineberger Canc Ctr, Chapel Hill, NC USA.
[Tsao, Hensin] Massachusetts Gen Hosp, Melanoma & Pigmented Lesion Ctr, Charlestown, MA USA.
[Tueting, Thomas] Univ Bonn, Dept Dermatol, Bonn, Germany.
[Lomax, Randy; Martin, Alison; Silverstein, Steve; Turnham, Tim] Melanoma Res Fdn, Washington, DC USA.
[Ronai, Ze'ev A.] Sanford Burnham Prebys Med Discovery Inst, Tumor Initiat & Maintenance Program, La Jolla, CA 92037 USA.
RP Ronai, ZA (reprint author), Sanford Burnham Prebys Med Discovery Inst, Tumor Initiat & Maintenance Program, La Jolla, CA 92037 USA.
EM ronai@SBPdiscovery.org
FU NCI NIH HHS [P30 CA016672, P30 CA010815, P50 CA121973]; NIAMS NIH HHS
[R01 AR043369]
NR 0
TC 6
Z9 6
U1 3
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1755-1471
EI 1755-148X
J9 PIGM CELL MELANOMA R
JI Pigment Cell Melanoma Res.
PD JUL
PY 2016
VL 29
IS 4
BP 404
EP 416
DI 10.1111/pcmr.12475
PG 13
WC Oncology; Cell Biology; Dermatology
SC Oncology; Cell Biology; Dermatology
GA DT2IC
UT WOS:000381303000005
PM 27087480
ER
PT J
AU George, AL
Boulanger, CA
Smith, GH
AF George, Andrea L.
Boulanger, Corinne A.
Smith, Gilbert H.
TI Telomerase and estrogen-sensing activities are essential for continued
mammary growth in vivo but dispensable for "reprogramming" neural stem
cells
SO AGING-US
LA English
DT Article
DE telomeres; mammary; senescence; stem cells
ID EPITHELIAL-CELLS; LIFE-SPAN; TRANSFORMING GROWTH-FACTOR-BETA-1; SERIAL
TRANSPLANTATION; TGF-BETA-1 EXPRESSION; RECEPTOR-ALPHA; IMMORTAL CELLS;
DEFICIENT MICE; MOUSE; GLAND
AB It has been proposed that the erosion of telomere length is a limiting factor in replicative capacity and important in cell senescence. To determine if this activity was essential in the mouse mammary gland in vivo, we serially transplanted mammary fragments from wild type (TER+/+), heterozygous (TER+/-), and homozygous (TER-/-) mammary tissues into the cleared mammary fat pads of immune-compromised nude mice. Individual implants from both homozygous and heterozygous TER null outgrowths showed growth senescence beginning at transplant generation two, earlier than implants from TER+/+ mammary glands which continued to show growth. This result suggests that either mammary epithelial stem cells maintain their telomere length in order to self renew, or that the absence or reduction of telomerase template results in more frequent death/extinction of stem cells during symmetric divisions. A third possibility is the inability of signaling cells in the niche to replicate resulting in reduction of the maintenance signals necessary for stem cell renewal. Consistent with this, examination of senescent outgrowths revealed the absence of estrogen receptor alpha (ER alpha(+)) epithelium although progesterone receptor (PR+) cells were abundant. Despite their inability to establish mammary growth in vivo, TER+/- cells were able to direct neural stem cells to mammary cell fates.
C1 [George, Andrea L.; Boulanger, Corinne A.; Smith, Gilbert H.] NCI, Basic Res Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
RP Smith, GH (reprint author), NCI, Basic Res Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
EM gs4d@nih.gov
FU intramural research program of the Center for Cancer Research NCI, NIH
FX The intramural research program of the Center for Cancer Research NCI,
NIH, supported this work.
NR 45
TC 0
Z9 0
U1 3
U2 3
PU IMPACT JOURNALS LLC
PI ALBANY
PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA
SN 1945-4589
J9 AGING-US
JI Aging-US
PD JUL
PY 2016
VL 8
IS 7
BP 1353
EP 1363
DI 10.18632/aging.100985
PG 11
WC Cell Biology
SC Cell Biology
GA DU1DV
UT WOS:000381949300008
PM 27347776
ER
PT J
AU Arrigoni, F
Peruzzo, D
Gagliardi, C
Maghini, C
Colombo, P
Iammarrone, FS
Pierpaoli, C
Triulzi, F
Turconi, AC
AF Arrigoni, F.
Peruzzo, D.
Gagliardi, C.
Maghini, C.
Colombo, P.
Iammarrone, F. Servodio
Pierpaoli, C.
Triulzi, F.
Turconi, A. C.
TI Whole-Brain DTI Assessment of White Matter Damage in Children with
Bilateral Cerebral Palsy: Evidence of Involvement beyond the Primary
Target of the Anoxic Insult
SO AMERICAN JOURNAL OF NEURORADIOLOGY
LA English
DT Article
ID GROSS MOTOR FUNCTION; PERIVENTRICULAR LEUKOMALACIA;
CLASSIFICATION-SYSTEM; FIBER-TRACKING; BORN PRETERM; TRACTOGRAPHY;
INJURY; IMPAIRMENT; TRACTS; ANISOTROPY
AB BACKGROUND AND PURPOSE: Cerebral palsy is frequently associated with both motor and nonmotor symptoms. DTI can characterize the damage at the level of motor tracts but provides less consistent results in nonmotor areas. We used a standardized pipeline of analysis to describe and quantify the pattern of DTI white matter abnormalities of the whole brain in a group of children with chronic bilateral cerebral palsy and periventricular leukomalacia. We also explored potential correlations between DTI and clinical scale metrics.
MATERIALS AND METHODS: Twenty-five patients (mean age, 11.8 years) and 25 healthy children (mean age, 11.8 years) were studied at 3T with a 2-mm isotropic DTI sequence. Differences between patients and controls were assessed both voxelwise and in ROIs obtained from an existing DTI atlas. Clinical metrics included the Gross Motor Function Classification System, the Manual Ability Classification System, and intelligence quotient.
RESULTS: The voxel-level and ROI-level analyses demonstrated highly significant (P < .001) modifications of DTI measurements in patients at several levels: cerebellar peduncles, corticospinal tracts and posterior thalamic radiations, posterior corpus callosum, external capsule, anterior thalamic radiation, superior longitudinal fasciculi and corona radiata, optic nerves, and chiasm. The reduction of fractional anisotropy values in significant tracts was between 8% and 30%. Statistically significant correlations were found between motor impairment and fractional anisotropy in corticospinal tracts and commissural and associative tracts of the supratentorial brain.
CONCLUSIONS: We demonstrated the involvement of several motor and nonmotor areas in the chronic damage associated with periventricular leukomalacia and showed new correlations between motor skills and DTI metrics.
C1 [Arrigoni, F.; Peruzzo, D.] Sci Inst IRCCS Eugenio Medea, Neuroimaging Lab, Bosisio Parini, Italy.
[Gagliardi, C.; Maghini, C.; Iammarrone, F. Servodio; Turconi, A. C.] Sci Inst IRCCS Eugenio Medea, Funct Neurorehabil Unit, Bosisio Parini, Italy.
[Colombo, P.] Sci Inst IRCCS Eugenio Medea, Child Psychopathol Unit, Bosisio Parini, Italy.
[Pierpaoli, C.] NIH, Bldg 10, Bethesda, MD 20892 USA.
[Triulzi, F.] Osped Maggiore Policlin, Fdn IRCCS Ca Granda, Dept Neuroradiol, Milan, Italy.
RP Arrigoni, F (reprint author), Sci Inst IRCCS Eugenio Medea, Via Don L Monza 20, I-23842 Bosisio Parini, Lecco, Italy.
EM filippo.arrigoni@bp.lnf.it
RI ARRIGONI, FILIPPO/G-5828-2011;
OI ARRIGONI, FILIPPO/0000-0002-5508-1149; Gagliardi,
Chiara/0000-0001-9931-9879
FU Italian Ministry of Health
FX This work was supported by the Italian Ministry of Health (Ricerca
Corrente 2012 to A.C. Turconi).
NR 36
TC 0
Z9 0
U1 0
U2 1
PU AMER SOC NEURORADIOLOGY
PI DENVILLE
PA PO BOX 3000, DENVILLE, NJ 07834-9349 USA
SN 0195-6108
EI 1936-959X
J9 AM J NEURORADIOL
JI Am. J. Neuroradiol.
PD JUL
PY 2016
VL 37
IS 7
BP 1347
EP 1353
DI 10.3174/ajnr.A4717
PG 7
WC Clinical Neurology; Neuroimaging; Radiology, Nuclear Medicine & Medical
Imaging
SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging
GA DS1NL
UT WOS:000380362600024
PM 26988814
ER
PT J
AU Sigurdson, AJ
Brenner, AV
Roach, JA
Goudeva, L
Muller, JA
Nerlich, K
Reiners, C
Schwab, R
Pfeiffer, L
Waldenberger, M
Braganza, M
Xu, L
Sturgis, EM
Yeager, M
Chanock, SJ
Pfeiffer, RM
Abend, M
Port, M
AF Sigurdson, Alice J.
Brenner, Alina V.
Roach, James A.
Goudeva, Lilia
Mueller, Joerg A.
Nerlich, Kai
Reiners, Christoph
Schwab, Robert
Pfeiffer, Liliane
Waldenberger, Melanie
Braganza, Melissa
Xu, Li
Sturgis, Erich M.
Yeager, Meredith
Chanock, Stephen J.
Pfeiffer, Ruth M.
Abend, Michael
Port, Matthias
TI Selected single-nucleotide polymorphisms in FOXE1, SERPINA5, FTO, EVPL,
TICAM1 and SCARB1 are associated with papillary and follicular thyroid
cancer risk: replication study in a German population
SO CARCINOGENESIS
LA English
DT Article
ID COMMON GENETIC-VARIANTS; CUMULATIVE RISK; POOLED ANALYSIS;
OVARIAN-CANCER; UNITED-STATES; CARCINOMA; OBESITY; SUSCEPTIBILITY;
DETERMINANT; RADIATION
AB We confirmed associations with papillary thyroid cancer risk and several FOXE1/HEMGN polymorphisms, as well as polymorphisms in SERPINA5, FTO, EVPL, TICAM1 and SCARB1. Polymorphisms in FOXE1, SERPINA5, FTO, TICAM1 and HSPA6 were also associated with risk of follicular thyroid cancer.Several single-nucleotide polymorphisms (SNPs) have been associated with papillary and follicular thyroid cancer (PTC and FTC, respectively) risk, but few have replicated. After analyzing 17525 tag SNPs in 1129 candidate genes, we found associations with PTC risk in SERPINA5, FTO, HEMGN (near FOXE1) and other genes. Here, we report results from a replication effort in a large independent PTC/FTC case-control study conducted in Germany. We evaluated the best tagging SNPs from our previous PTC study and additionally included SNPs in or near FOXE1 and NKX2-1 genes, known susceptibility loci for thyroid cancer. We genotyped 422 PTC and 130 FTC cases and 752 controls recruited from three German clinical centers. We used polytomous logistic regression to simultaneously estimate PTC and FTC associations for 79 SNPs based on log-additive models. We assessed effect modification by body mass index (BMI), gender and age for all SNPs, and selected SNP by SNP interactions. We confirmed associations with PTC and SNPs in FOXE1/HEMGN, SERPINA5 (rs2069974), FTO (rs8047395), EVPL (rs2071194), TICAM1 (rs8120) and SCARB1 (rs11057820) genes. We found associations with SNPs in FOXE1, SERPINA5, FTO, TICAM1 and HSPA6 and FTC. We found two significant interactions between FTO (rs8047395) and BMI (P = 0.0321) and between TICAM1 (rs8120) and FOXE1 (rs10984377) (P = 0.0006). Besides the known associations with FOXE1 SNPs, we confirmed additional PTC SNP associations reported previously. We also found several new associations with FTC risk and noteworthy interactions. We conclude that multiple variants and host factors might interact in complex ways to increase risk of PTC and FTC.
C1 [Sigurdson, Alice J.; Brenner, Alina V.; Roach, James A.; Braganza, Melissa] NCI, Radiat Epidemiol Branch, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA.
[Goudeva, Lilia] Hannover Med Sch, Dept Transfus Med, Hannover, Germany.
[Mueller, Joerg A.] Hannover Med Sch, Dept Nucl Med, Hannover, Germany.
[Nerlich, Kai; Reiners, Christoph] Univ Hosp Wurzburg, Dept Nucl Med, Wurzburg, Germany.
[Schwab, Robert] Fed Armed Forces Hosp, Dept Surg, Koblenz, Germany.
[Pfeiffer, Liliane; Waldenberger, Melanie] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Res Unit Mol Epidemiol, Neuherberg, Germany.
[Pfeiffer, Liliane; Waldenberger, Melanie] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Inst Epidemiol 2, Neuherberg, Germany.
[Xu, Li; Sturgis, Erich M.] Univ Texas MD Anderson Canc Ctr, Dept Head & Neck Surg, Houston, TX 77030 USA.
[Yeager, Meredith; Chanock, Stephen J.] NCI, Lab Translat Genet, Bethesda, MD 20892 USA.
[Pfeiffer, Ruth M.] NCI, Biostat Branch, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA.
[Abend, Michael] Univ Ulm, Bundeswehr Inst Radiobiol, Neuherbergstr 11, D-80937 Munich, Germany.
[Port, Matthias] Hannover Med Sch, Dept Hematol Hemostasis Oncol & Stem Cell Transpl, Hannover, Germany.
RP Abend, M (reprint author), Univ Ulm, Bundeswehr Inst Radiobiol, Neuherbergstr 11, D-80937 Munich, Germany.
EM michaelabend@bundeswehr.org
RI Waldenberger, Melanie/B-5355-2014
OI Waldenberger, Melanie/0000-0003-0583-5093
FU Intramural Research Program of the Division of Cancer Epidemiology and
Genetics, National Cancer Institute, National Institutes of Health;
German Ministry of Defense
FX Intramural Research Program of the Division of Cancer Epidemiology and
Genetics, National Cancer Institute, National Institutes of Health;
German Ministry of Defense.
NR 41
TC 2
Z9 2
U1 1
U2 1
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0143-3334
EI 1460-2180
J9 CARCINOGENESIS
JI Carcinogenesis
PD JUL
PY 2016
VL 37
IS 7
BP 677
EP 684
DI 10.1093/carcin/bgw047
PG 8
WC Oncology
SC Oncology
GA DT0XJ
UT WOS:000381206000004
PM 27207655
ER
PT J
AU Bassig, BA
Zhang, LP
Vermeulen, R
Tang, XJ
Li, GL
Hu, W
Guo, WH
Purdue, MP
Yin, SN
Rappaport, SM
Shen, M
Ji, ZY
Qiu, CY
Ge, YC
Hosgood, HD
Reiss, B
Wu, BH
Xie, YX
Li, LY
Yue, F
Freeman, LEB
Blair, A
Hayes, RB
Huang, HL
Smith, MT
Rothman, N
Lan, Q
AF Bassig, Bryan A.
Zhang, Luoping
Vermeulen, Roel
Tang, Xiaojiang
Li, Guilan
Hu, Wei
Guo, Weihong
Purdue, Mark P.
Yin, Songnian
Rappaport, Stephen M.
Shen, Min
Ji, Zhiying
Qiu, Chuangyi
Ge, Yichen
Hosgood, H. Dean
Reiss, Boris
Wu, Banghua
Xie, Yuxuan
Li, Laiyu
Yue, Fei
Freeman, Laura E. Beane
Blair, Aaron
Hayes, Richard B.
Huang, Hanlin
Smith, Martyn T.
Rothman, Nathaniel
Lan, Qing
TI Comparison of hematological alterations and markers of B-cell activation
in workers exposed to benzene, formaldehyde and trichloroethylene
SO CARCINOGENESIS
LA English
DT Article
ID MYELOID PROGENITOR CELLS; NON-HODGKIN-LYMPHOMA; OCCUPATIONAL-EXPOSURE;
CHINESE WORKERS; RISK; CANCER; METAANALYSIS; HEMATOTOXICITY; LEUKEMIA;
COHORT
AB The current study demonstrates differences in how cells derived from the myeloid versus the lymphoid lineage are affected by exposures to benzene, formaldeyde and TCE, all of which have been associated with specific hematologic cancers in epidemiologic studies.Benzene, formaldehyde (FA) and trichloroethylene (TCE) are ubiquitous chemicals in workplaces and the general environment. Benzene is an established myeloid leukemogen and probable lymphomagen. FA is classified as a myeloid leukemogen but has not been associated with non-Hodgkin lymphoma (NHL), whereas TCE has been associated with NHL but not myeloid leukemia. Epidemiologic associations between FA and myeloid leukemia, and between benzene, TCE and NHL are, however, still debated. Previously, we showed that these chemicals are associated with hematotoxicity in cross-sectional studies of factory workers in China, which included extensive personal monitoring and biological sample collection. Here, we compare and contrast patterns of hematotoxicity, monosomy 7 in myeloid progenitor cells (MPCs), and B-cell activation biomarkers across these studies to further evaluate possible mechanisms of action and consistency of effects with observed hematologic cancer risks. Workers exposed to benzene or FA, but not TCE, showed declines in cell types derived from MPCs, including granulocytes and platelets. Alterations in lymphoid cell types, including B cells and CD4+ T cells, and B-cell activation markers were apparent in workers exposed to benzene or TCE. Given that alterations in myeloid and lymphoid cell types are associated with hematological malignancies, our data provide biologic insight into the epidemiological evidence linking benzene and FA exposure with myeloid leukemia risk, and TCE and benzene exposure with NHL risk.
C1 [Bassig, Bryan A.; Hu, Wei; Purdue, Mark P.; Shen, Min; Freeman, Laura E. Beane; Blair, Aaron; Rothman, Nathaniel; Lan, Qing] NCI, Occupat & Environm Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, Rockville, MD USA.
[Zhang, Luoping; Guo, Weihong; Rappaport, Stephen M.; Ji, Zhiying; Smith, Martyn T.] Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.
[Vermeulen, Roel] Univ Utrecht, Inst Risk Assessment Sci, Utrecht, Netherlands.
[Tang, Xiaojiang; Qiu, Chuangyi; Ge, Yichen; Wu, Banghua; Xie, Yuxuan; Li, Laiyu; Yue, Fei; Huang, Hanlin] Guangdong Poison Control Ctr, Guangzhou, Guangdong, Peoples R China.
[Li, Guilan; Yin, Songnian] Chinese Ctr Dis Control & Prevent, Inst Occupat Hlth & Poison Control, Beijing, Peoples R China.
[Hosgood, H. Dean] Albert Einstein Coll Med, Dept Epidemiol & Populat Hlth, Bronx, NY 10467 USA.
[Reiss, Boris] Univ Washington, Dept Environm & Occupat Hlth Sci, Seattle, WA 98195 USA.
[Hayes, Richard B.] NYU, Dept Environm Med, Sch Med, Div Epidemiol, 550 1St Ave, New York, NY 10016 USA.
RP Bassig, BA; Lan, Q (reprint author), NCI, Occupat & Environm Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, Rockville, MD USA.; Zhang, LP (reprint author), Univ Calif Berkeley, Sch Publ Hlth, Div Environm Hlth Sci, Berkeley, CA 94720 USA.
EM bryan.bassig@nih.gov; luoping@berkeley.edu; qingl@mail.nih.gov
RI Vermeulen, Roel/F-8037-2011;
OI Vermeulen, Roel/0000-0003-4082-8163; Hayes, Richard/0000-0002-0918-661X
FU National Cancer Institute; National Institute of Environmental Health
Sciences [P42ES04705, P30ES01896, R01ES017452]; Northern California
Center for Occupational and Environmental Health; Department of Science
and Technology of Guangdong Province, China [2007A050100004]; National
Institutes of Health [P42ES04705, P30ES01896, RO1ES06721, P42ES05948,
P30ES10126, N01-CO-12400]; SAIC-Frederick, Inc.
FX TCE study: National Institutes of Health and National Cancer Institute;
National Institute of Environmental Health Sciences (P42ES04705,
P30ES01896); Northern California Center for Occupational and
Environmental Health; Department of Science and Technology of Guangdong
Province, China (2007A050100004). FA study: National Cancer Institute;
National Institute of Environmental Health Sciences (P42ES04705,
R01ES017452); the Northern California Center for Occupational and
Environmental Health; Department of Science and Technology of Guangdong
Province, China (2007A050100004). Benzene study: National Institutes of
Health (RO1ES06721, P42ES04705, P30ES01896, P42ES05948, P30ES10126) and
National Institutes of Health contract N01-CO-12400 with SAIC-Frederick,
Inc.
NR 46
TC 1
Z9 1
U1 3
U2 4
PU OXFORD UNIV PRESS
PI OXFORD
PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
SN 0143-3334
EI 1460-2180
J9 CARCINOGENESIS
JI Carcinogenesis
PD JUL
PY 2016
VL 37
IS 7
BP 692
EP 700
DI 10.1093/carcin/bgw053
PG 9
WC Oncology
SC Oncology
GA DT0XJ
UT WOS:000381206000006
PM 27207665
ER
PT J
AU Williams, R
White, P
Nieto, J
Vieira, D
Francois, F
Hamilton, F
AF Williams, Renee
White, Pascale
Nieto, Jose
Vieira, Dorice
Francois, Fritz
Hamilton, Frank
TI Colorectal Cancer in African Americans: An Update
SO CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
LA English
DT Review
ID BLACK-WHITE DIFFERENCES; RANDOMIZED-CLINICAL-TRIAL; PRIMARY-CARE;
RACIAL-DIFFERENCES; UNITED-STATES; SCREENING COLONOSCOPY;
SOCIOECONOMIC-STATUS; ETHNIC DISPARITIES; HEALTH DISPARITIES;
RECTAL-CANCER
AB This review is an update to the American College of Gastroenterology (ACG) Committee on Minority Affairs and Cultural Diversity's paper on colorectal cancer (CRC) in African Americans published in 2005. Over the past 10 years, the incidence and mortality rates of CRC in the United States has steadily declined. However, reductions have been strikingly much slower among African Americans who continue to have the highest rate of mortality and lowest survival when compared with all other racial groups. The reasons for the health disparities are multifactorial and encompass physician and patient barriers. Patient factors that contribute to disparities include poor knowledge of benefits of CRC screening, limited access to health care, insurance status along with fear and anxiety. Physician factors include lack of knowledge of screening guidelines along with disparate recommendations for screening. Earlier screening has been recommended as an effective strategy to decrease observed disparities; currently the ACG and American Society of Gastrointestinal Endoscopists recommend CRC screening in African Americans to begin at age 45. Despite the decline in CRC deaths in all racial and ethnic groups, there still exists a significant burden of CRC in African Americans, thus other strategies including educational outreach for health care providers and patients and the utilization of patient navigation systems emphasizing the importance of screening are necessary. These strategies have been piloted in both local communities and Statewide resulting in notable significant decreases in observed disparities.
C1 [Williams, Renee; Vieira, Dorice; Francois, Fritz] NYU, Sch Med, Bellevue Hosp Ctr, 462 First Ave,Room CD 697, New York, NY 10016 USA.
[White, Pascale] Mem Sloan Kettering Canc Ctr, 1275 York Ave, New York, NY 10021 USA.
[Nieto, Jose] Borland Groover Clin, Jacksonville, FL USA.
[Hamilton, Frank] NIH, Bldg 10, Bethesda, MD 20892 USA.
RP Williams, R (reprint author), NYU, Sch Med, Bellevue Hosp Ctr, 462 First Ave,Room CD 697, New York, NY 10016 USA.
EM Renee.Williams@nyumc.org
OI Francois, Fritz/0000-0003-4491-2637; Williams,
Renee/0000-0001-5530-2207; Vieira, Dorice/0000-0003-4232-9413
NR 95
TC 0
Z9 0
U1 3
U2 3
PU NATURE PUBLISHING GROUP
PI NEW YORK
PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA
SN 2155-384X
J9 CLIN TRANSL GASTROEN
JI Clin. Transl. Gastroenterol.
PD JUL
PY 2016
VL 7
AR e185
DI 10.1038/ctg.2016.36
PG 9
WC Gastroenterology & Hepatology
SC Gastroenterology & Hepatology
GA DU3CD
UT WOS:000382087500008
PM 27467183
ER
PT J
AU Catanzaro, D
Shackney, SE
Schaffer, AA
Schwartz, R
AF Catanzaro, Daniele
Shackney, Stanley E.
Schaffer, Alejandro A.
Schwartz, Russell
TI Classifying the Progression of Ductal Carcinoma from Single-Cell Sampled
Data via Integer Linear Programming: A Case Study
SO IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
LA English
DT Article
DE Tumor profiling; single-cell sequencing; ductal carcinoma of the breast;
phylogeny estimation; parsimony criterion; computational biology;
distance methods; network design; combinatorial optimization; mixed
integer linear programming
ID HETEROGENEOUS BREAST-TUMORS; IN-SITU; CANCER; EVOLUTION; GENOME;
PATHWAYS; AMPLIFICATION; GLIOBLASTOMA; HALLMARKS; INFERENCE
AB Ductal Carcinoma In Situ (DCIS) is a precursor lesion of Invasive Ductal Carcinoma (IDC) of the breast. Investigating its temporal progression could provide fundamental new insights for the development of better diagnostic tools to predict which cases of DCIS will progress to IDC. We investigate the problem of reconstructing a plausible progression from single-cell sampled data of an individual with synchronous DCIS and IDC. Specifically, by using a number of assumptions derived from the observation of cellular atypia occurring in IDC, we design a possible predictive model using integer linear programming (ILP). Computational experiments carried out on a preexisting data set of 13 patients with simultaneous DCIS and IDC show that the corresponding predicted progression models are classifiable into categories having specific evolutionary characteristics. The approach provides new insights into mechanisms of clonal progression in breast cancers and helps illustrate the power of the ILP approach for similar problems in reconstructing tumor evolution scenarios under complex sets of constraints.
C1 [Catanzaro, Daniele] Catholic Univ Louvain, Louvain Sch Management, B-1517000 Mons, Belgium.
[Catanzaro, Daniele] Catholic Univ Louvain, Ctr Operat Res & Econometr, B-1517000 Mons, Belgium.
[Shackney, Stanley E.] Drexel Univ, Sch Med, Dept Human Oncol, Pittsburgh, PA 15243 USA.
[Shackney, Stanley E.] Drexel Univ, Sch Med, Dept Human Genet, Pittsburgh, PA 15243 USA.
[Schaffer, Alejandro A.] NCBI, Computat Biol Branch, NIH, Bethesda, MD 20894 USA.
[Schwartz, Russell] Carnegie Mellon Univ, Dept Biol Sci, Pittsburgh, PA USA.
[Schwartz, Russell] Carnegie Mellon Univ, Computat Biol Dept, Pittsburgh, PA USA.
RP Catanzaro, D (reprint author), Catholic Univ Louvain, Louvain Sch Management, B-1517000 Mons, Belgium.; Catanzaro, D (reprint author), Catholic Univ Louvain, Ctr Operat Res & Econometr, B-1517000 Mons, Belgium.
EM daniele.catanzaro@uclouvain.be; aschaffe@helix.nih.gov;
russells@andrew.cmu.edu
RI Schwartz, Russell/A-1998-2016;
OI Schwartz, Russell/0000-0002-4970-2252; Catanzaro,
Daniele/0000-0001-9427-1562
FU Belgian National Fund for Scientific Research (FRS-FNRS); US National
Institutes of Health [1R01CA140214, 1R01AI076318]; Intramural Research
Program of the NIH, NLM
FX This research was supported in part by the Belgian National Fund for
Scientific Research (FRS-FNRS) (D.C), US National Institutes of Health
grants 1R01CA140214 (R.S.) and 1R01AI076318 (R.S.), and the Intramural
Research Program of the NIH, NLM (A.A.S.). The authors thank the
anonymous reviewers whose insightful suggestions helped them improve the
manuscript.
NR 50
TC 1
Z9 1
U1 2
U2 3
PU IEEE COMPUTER SOC
PI LOS ALAMITOS
PA 10662 LOS VAQUEROS CIRCLE, PO BOX 3014, LOS ALAMITOS, CA 90720-1314 USA
SN 1545-5963
EI 1557-9964
J9 IEEE ACM T COMPUT BI
JI IEEE-ACM Trans. Comput. Biol. Bioinform.
PD JUL-AUG
PY 2016
VL 13
IS 4
BP 643
EP 655
DI 10.1109/TCBB.2015.2476808
PG 13
WC Biochemical Research Methods; Computer Science, Interdisciplinary
Applications; Mathematics, Interdisciplinary Applications; Statistics &
Probability
SC Biochemistry & Molecular Biology; Computer Science; Mathematics
GA DT5FI
UT WOS:000381506500005
PM 26353381
ER
PT J
AU Miller, A
Yeskey, K
Garantziotis, S
Arnesen, S
Bennett, A
O'Fallon, L
Thompson, C
Reinlib, L
Masten, S
Remington, J
Love, C
Ramsey, S
Rosselli, R
Galluzzo, B
Lee, J
Kwok, R
Hughes, J
AF Miller, Aubrey
Yeskey, Kevin
Garantziotis, Stavros
Arnesen, Stacey
Bennett, April
O'Fallon, Liam
Thompson, Claudia
Reinlib, Les
Masten, Scott
Remington, James
Love, Cindy
Ramsey, Steve
Rosselli, Richard
Galluzzo, Betsy
Lee, Joy
Kwok, Richard
Hughes, Joseph
TI Integrating Health Research into Disaster Response: The New NIH Disaster
Research Response Program
SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
LA English
DT Editorial Material
DE disasters; disaster research; disaster epidemiology; environmental
health; science preparedness; disaster risk reduction; strategic science
ID COMMUNITY; EPIDEMIOLOGY; TOOL
AB The need for high quality and timely disaster research has been a topic of great discussion over the past several years. Recent high profile incidents have exposed gaps in knowledge about the health impacts of disasters or the benefits of specific interventions-such was the case with the 2010 Gulf Oil Spill and recent events associated with lead-contaminated drinking water in Flint, Michigan, and the evolving health crisis related to Zika virus disease. Our inability to perform timely research to inform the community about health and safety risks or address specific concerns further heightens anxiety and distrust. Since nearly all disasters, whether natural or man-made, have an environmental health component, it is critical that specialized research tools and trained researchers be readily available to evaluate complex exposures and health effects, especially for vulnerable sub-populations such as the elderly, children, pregnant women, and those with socioeconomic and environmental disparities. In response, the National Institute of Environmental Health Science has initiated a Disaster Research Response Program to create new tools, protocols, networks of researchers, training exercises, and outreach involving diverse groups of stakeholders to help overcome the challenges of disaster research and to improve our ability to collect vital information to reduce the adverse health impacts and improve future preparedness.
C1 [Miller, Aubrey; Bennett, April] NIEHS, Bethesda, MD 20892 USA.
[Yeskey, Kevin; Galluzzo, Betsy; Lee, Joy] MDB Inc, Washington, DC 20036 USA.
[Garantziotis, Stavros; O'Fallon, Liam; Thompson, Claudia; Reinlib, Les; Masten, Scott; Remington, James; Kwok, Richard; Hughes, Joseph] NIEHS, Res Triangle Pk, NC 27709 USA.
[Arnesen, Stacey; Love, Cindy] Natl Lib Med, Bethesda, MD 20892 USA.
[Ramsey, Steve; Rosselli, Richard] Social & Sci Syst Inc, Durham, NC 27703 USA.
RP Miller, A (reprint author), NIEHS, Bethesda, MD 20892 USA.
EM miller.aubrey@nih.gov; kyeskey@michaeldbaker.com;
garantziotis@niehs.nih.gov; arneses@mail.nlm.nih.gov;
april.bennett@nih.gov; ofallon@niehs.nih.gov; thompso1@niehs.nih.gov;
reinlib@niehs.nih.gov; masten@niehs.nih.gov; remingtonj@niehs.nih.gov;
lovec@mail.nlm.nih.gov; SRamsey@s-3.com; RRosselli@s-3.com;
beagin@michaeldbaker.com; jlee@michaeldbaker.com; Richard.Kwok@nih.gov;
hughes3@niehs.nih.gov
RI masten, scott/R-1403-2016; Garantziotis, Stavros/A-6903-2009; Kwok,
Richard/B-6907-2017
OI masten, scott/0000-0002-7847-181X; Garantziotis,
Stavros/0000-0003-4007-375X; Kwok, Richard/0000-0002-6794-8360
NR 14
TC 0
Z9 0
U1 57
U2 65
PU MDPI AG
PI BASEL
PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
SN 1660-4601
J9 INT J ENV RES PUB HE
JI Int. J. Environ. Res. Public Health
PD JUL
PY 2016
VL 13
IS 7
AR 676
DI 10.3390/ijerph13070676
PG 12
WC Environmental Sciences; Public, Environmental & Occupational Health
SC Environmental Sciences & Ecology; Public, Environmental & Occupational
Health
GA DS4OG
UT WOS:000380759800052
ER
PT J
AU Zaleski, RT
Egeghy, PP
Hakkinen, PJ
AF Zaleski, Rosemary T.
Egeghy, Peter P.
Hakkinen, Pertti J.
TI Exploring Global Exposure Factors Resources for Use in Consumer Exposure
Assessments
SO INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
LA English
DT Review
DE consumer products; exposure factors; databases; exposure assessments;
online; time-activity patterns; consumer behavior; household products
ID PERSONAL CARE PRODUCTS; NONDIETARY INGESTION EXPOSURE; MOUTH FREQUENCY
DATA; FACTORS HANDBOOK; COSMETIC PRODUCTS; RISK-ASSESSMENT; 21ST-CENTURY
ROADMAP; YOUNG-CHILDREN; INDOOR AIR; CHEMICALS
AB This publication serves as a global comprehensive resource for readers seeking exposure factor data and information relevant to consumer exposure assessment. It describes the types of information that may be found in various official surveys and online and published resources. The relevant exposure factors cover a broad range, including general exposure factor data found in published compendia and databases and resources about specific exposure factors, such as human activity patterns and housing information. Also included are resources on exposure factors related to specific types of consumer products and the associated patterns of use, such as for a type of personal care product or a type of children's toy. Further, a section on using exposure factors for designing representative exposure scenarios is included, along with a look into the future for databases and other exposure science developments relevant for consumer exposure assessment.
C1 [Zaleski, Rosemary T.] ExxonMobil Biomed Sci Inc, 1545 Route 22 East, Annandale, NJ 08801 USA.
[Egeghy, Peter P.] US EPA, Off Res & Dev, Natl Exposure Res Lab, Res Triangle Pk, NC 27711 USA.
[Hakkinen, Pertti J.] Natl Lib Med, NIH, 6707 Democracy Blvd,Suite 510, Bethesda, MD 20894 USA.
RP Zaleski, RT (reprint author), ExxonMobil Biomed Sci Inc, 1545 Route 22 East, Annandale, NJ 08801 USA.
EM rosemary.t.zaleski@exxonmobil.com; egeghy.peter@epa.gov;
pertti.hakkinen@nih.gov
FU ExxonMobil Biomedical Sciences, Inc.
FX The International Society of Exposure Science Symposium on Exploring
Global Data Resources for Consumer Exposure Assessment served both as
the inspiration for developing this publication, as well as the basis
for much of the information provided in the document (costs of open
access were paid for by ExxonMobil Biomedical Sciences, Inc.). The
authors (symposium co-organizers) would like to thank all of the
conference presenters for the information shared both at the symposium
and for inclusion in this paper, as well as help in manuscript review,
by recognizing them as contributors below.
NR 139
TC 0
Z9 0
U1 3
U2 3
PU MDPI AG
PI BASEL
PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
SN 1660-4601
J9 INT J ENV RES PUB HE
JI Int. J. Environ. Res. Public Health
PD JUL
PY 2016
VL 13
IS 7
AR 744
DI 10.3390/ijerph13070744
PG 26
WC Environmental Sciences; Public, Environmental & Occupational Health
SC Environmental Sciences & Ecology; Public, Environmental & Occupational
Health
GA DS4OG
UT WOS:000380759800120
ER
PT J
AU Dong, BX
Somani, AK
Love, PE
Zheng, X
Chen, XQ
Zhang, JY
AF Dong, Baoxia
Somani, Ally-Khan
Love, Paul E.
Zheng, Xuan
Chen, Xiequn
Zhang, Jinyi
TI CD5-mediated inhibition of TCR signaling proceeds normally in the
absence of SHP-1
SO INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
LA English
DT Article
DE T-cell antigen receptor signaling; tyrosine phosphatase; thymic
selection; CD5; SHP-1
ID CELL ANTIGEN RECEPTOR; CYTOPLASMIC FREE CALCIUM; PROTEIN-KINASE-C;
TYROSINE-PHOSPHATASE; NEGATIVE REGULATION; T-CELLS; B-CELLS;
PHOSPHATIDYLINOSITOL 3-KINASE; CD5 EXPRESSION; ACTIVATION
AB The CD5 transmembrane glycoprotein functions as a co-receptor in the signaling pathway linking T-cell antigen receptor (TCR) engagement to activation and differentiation. Although CD5 effects on TCR signaling have been shown to be primarily inhibitory, the underlying mechanisms remain unclear. In view of recent data revealing the ability of CD5 to associate with the SHP-1 tyrosine phosphatase, a protein that also downregulates TCR signaling, we examined the role of SHP-1 in modulating CD5 function using thymocytes from SHP-1-deficient viable motheaten (me(v)) mice. The results revealed the association of SHP-1 with CD5 to be markedly increased following TCR stimulation and indicated that this interaction was enhanced by and was dependent on CD5 tyrosine phosphorylation. However, there was no difference of the tyrosine phosphorylation status of CD5 between resting and TCR-stimulated cells in SHP-1-deficient compared to wildtype thymocytes. Lack of SHP-1 activity did not affect the levels of CD5 surface expression, CD5 co-immunoprecipitable tyrosine phosphatase activity and intracellular calcium increase following co-crosslinking of the TCR and CD5. Similarly, an analysis of T-cell thymocyte populations in me(v) mice expressing an H-Y transgene as well as a construct mediating T-cell restricted CD5 overexpression, revealed that the reduction in the positive selection conferred by CD5 overexpression was unaffected by SHP-1 deficiency. CD5 is not a SHP-1 substrate and SHP-1 is not required for and possibly not involved in the CD5-mediated modulation of TCR signaling.
C1 [Dong, Baoxia; Zheng, Xuan; Chen, Xiequn] Fourth Mil Med Univ, Xijing Hosp, Dept Haematol, Xian 710032, Shaanxi, Peoples R China.
[Somani, Ally-Khan; Zhang, Jinyi] Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON M5G 1X5, Canada.
[Love, Paul E.] NICHHD, Lab Mammalian Genes & Dev, NIH, Bethesda, MD 20892 USA.
[Somani, Ally-Khan] Indiana Univ Sch Med, Dept Dermatol, Indianapolis, IN 46202 USA.
RP Dong, BX (reprint author), Fourth Mil Med Univ, Xijing Hosp, Dept Haematol, Xian 710032, Shaanxi, Peoples R China.; Zhang, JY (reprint author), Mt Sinai Hosp, Lunenfeld Tanenbaum Res Inst, Toronto, ON M5G 1X5, Canada.
EM dongbaoxia18@163.com; jinyi@mshri.on.ca
NR 55
TC 0
Z9 0
U1 2
U2 2
PU SPANDIDOS PUBL LTD
PI ATHENS
PA POB 18179, ATHENS, 116 10, GREECE
SN 1107-3756
EI 1791-244X
J9 INT J MOL MED
JI Int. J. Mol. Med.
PD JUL
PY 2016
VL 38
IS 1
BP 45
EP 56
DI 10.3892/ijmm.2016.2592
PG 12
WC Medicine, Research & Experimental
SC Research & Experimental Medicine
GA DT2CZ
UT WOS:000381289700006
PM 27221212
ER
PT J
AU Campbell, CMP
Viscidi, RP
Torres, BN
Lin, HY
Fulp, W
Abrahamsen, M
Lazcano-Ponce, E
Villa, LL
Kreimer, AR
Giuliano, AR
AF Campbell, Christine M. Pierce
Viscidi, Raphael P.
Torres, B. Nelson
Lin, Hui-Yi
Fulp, William
Abrahamsen, Martha
Lazcano-Ponce, Eduardo
Villa, Luisa L.
Kreimer, Aimee R.
Giuliano, Anna R.
TI Human Papillomavirus (HPV) L1 Serum Antibodies and the Risk of
Subsequent Oral HPV Acquisition in Men: The HIM Study
SO JOURNAL OF INFECTIOUS DISEASES
LA English
DT Article
DE oral HPV; HPV16; antibodies; serology; men
ID CANCER INCIDENCE; NATURAL-HISTORY; INFECTION; VACCINATION; RESPONSES;
COHORT
AB The role of antibody-mediated immunity in preventing newly acquired oral human papillomavirus (HPV) is not well understood. Among 1618 men participating in the HPV Infection in Men (HIM) Study, we evaluated oral rinses for HPV DNA and baseline sera for HPV-6, -11, -16, and -18 L1 antibodies. Thirty percent of men (486) were seropositive for >= 1 HPV type, and 25 men developed incident oral HPV infection (HPV-6 was detected in 7, HPV-11 in 0, HPV-16 in 17, and HPV-18 in 1). Cox models revealed that men with circulating antibodies to HPV-6, -11, -16, or -18 were not less likely to acquire type-specific oral HPV than men without antibodies (hazard ratio for the risk of acquiring HPV-6, -11, -16, or -18, 1.63; 95% confidence interval,.56-4.76).
C1 [Campbell, Christine M. Pierce; Torres, B. Nelson; Abrahamsen, Martha; Giuliano, Anna R.] H Lee Moffitt Canc Ctr & Res Inst, Dept Canc Epidemiol, Tampa, FL USA.
[Lin, Hui-Yi; Fulp, William] H Lee Moffitt Canc Ctr & Res Inst, Dept Biostat & Bioinformat, Tampa, FL USA.
[Viscidi, Raphael P.] Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA.
[Kreimer, Aimee R.] NCI, Div Canc Epidemiol & Genet, NIH, Bethesda, MD 20892 USA.
[Lazcano-Ponce, Eduardo] Inst Nacl Salud Publ, Ctr Invest Salud Poblac, Cuernavaca, Morelos, Mexico.
[Villa, Luisa L.] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, BR-05508 Sao Paulo, Brazil.
[Villa, Luisa L.] Canc Inst State Sao Paulo, Sao Paulo, Brazil.
[Lin, Hui-Yi] Louisiana State Univ, Sch Publ Hlth, Hlth Sci Ctr, Dept Biostat, New Orleans, LA USA.
[Fulp, William] Fred Hutchinson Canc Res Ctr, Stat Ctr HIV AIDS Res & Prevent, 1124 Columbia St, Seattle, WA 98104 USA.
RP Giuliano, AR (reprint author), MRC CANCONT, Ctr Infect Res Canc, 12902 Magnolia Dr, Tampa, FL 33612 USA.
EM anna.giuliano@moffitt.org
FU NCI, NIH [R01 CA098803]; National Cancer Institute Intramural Program;
Investigator-Initiated Studies Program, Merck Sharp and Dohme; American
Cancer Society [PF-13-222-01-MPC]
FX This work was supported by the NCI, NIH (grant R01 CA098803 to A. R.
G.); the National Cancer Institute Intramural Program (to A. R. K.); the
Investigator-Initiated Studies Program, Merck Sharp and Dohme (to A. R.
G.); and the American Cancer Society (postdoctoral fellowship
PF-13-222-01-MPC to C. M. P. C.).
NR 15
TC 0
Z9 0
U1 2
U2 2
PU OXFORD UNIV PRESS INC
PI CARY
PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA
SN 0022-1899
EI 1537-6613
J9 J INFECT DIS
JI J. Infect. Dis.
PD JUL 1
PY 2016
VL 214
IS 1
BP 45
EP 48
DI 10.1093/infdis/jiw083
PG 4
WC Immunology; Infectious Diseases; Microbiology
SC Immunology; Infectious Diseases; Microbiology
GA DR3SF
UT WOS:000379822200008
ER
PT J
AU Beigi, RH
Noguchi, L
Brown, G
Piper, J
Watts, DH
AF Beigi, Richard H.
Noguchi, Lisa
Brown, Gina
Piper, Jeanna
Watts, D. Heather
TI Performing Drug Safety Research During Pregnancy and Lactation:
Biomedical HIV Prevention Research as a Template
SO JOURNAL OF WOMENS HEALTH
LA English
DT Article
ID TO-CHILD TRANSMISSION; PREEXPOSURE PROPHYLAXIS; PRETERM DELIVERY; WOMEN;
RISK; INFECTION; MEDICATIONS; UGANDA; TERM; PHARMACOKINETICS
AB Evidence-based guidance regarding use of nearly all pharmaceuticals by pregnant and lactating women is limited. Models for performing research may assist in filling these knowledge gaps. Internationally, reproductive age women are at high risk of human immunodeficiency virus (HIV) acquisition. Susceptibility to HIV infection may be increased during pregnancy, and risk of maternal-child transmission is increased with incident HIV infection during pregnancy and lactation. A multidisciplinary meeting of experts was convened at the United States National Institutes of Health to consider paradigms for drug research in pregnancy and lactation applicable to HIV prevention. This report summarizes the meeting proceedings and describes a framework for research on candidate HIV prevention agent use during pregnancy and lactation that may also have broader applications to other pharmaceutical products.
C1 [Beigi, Richard H.] Univ Pittsburgh, Med Ctr, Div Reprod Infect Dis, Dept Obstet Gynecol & Reprod Sci,Magee Womens, 300 Halket St, Pittsburgh, PA 15213 USA.
[Beigi, Richard H.; Noguchi, Lisa] Magee Womens Res Inst, Microbicide Trials Network, Pittsburgh, PA USA.
[Noguchi, Lisa] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA.
[Brown, Gina] NIH, Off AIDS Res, Bldg 10, Bethesda, MD 20892 USA.
[Piper, Jeanna] NIAID, Div Aids, NIH, Bethesda, MD 20892 USA.
[Watts, D. Heather] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, NIH, Bethesda, MD USA.
RP Beigi, RH (reprint author), Univ Pittsburgh, Med Ctr, Div Reprod Infect Dis, Dept Obstet Gynecol & Reprod Sci,Magee Womens, 300 Halket St, Pittsburgh, PA 15213 USA.
EM rbeigi@mail.magee.edu
FU National Institute of Allergy and Infectious Diseases, Division of AIDS,
National Institutes of Health; Eunice Kennedy Shriver National Institute
of Child Health and Human Development, National Institutes of Health,
Bethesda, Maryland
FX Funding for the meeting was provided by National Institute of Allergy
and Infectious Diseases, Division of AIDS, National Institutes of
Health, and the Eunice Kennedy Shriver National Institute of Child
Health and Human Development, National Institutes of Health, Bethesda,
Maryland. All authors planned and attended the meeting, reviewed all of
the relevant literature, and reviewed all drafts of the manuscript.
NR 48
TC 1
Z9 1
U1 2
U2 2
PU MARY ANN LIEBERT, INC
PI NEW ROCHELLE
PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA
SN 1540-9996
EI 1931-843X
J9 J WOMENS HEALTH
JI J. Womens Health
PD JUL
PY 2016
VL 25
IS 7
BP 761
EP 766
DI 10.1089/jwh.2013.4398
PG 6
WC Public, Environmental & Occupational Health; Medicine, General &
Internal; Obstetrics & Gynecology; Women's Studies
SC Public, Environmental & Occupational Health; General & Internal
Medicine; Obstetrics & Gynecology; Women's Studies
GA DS5GB
UT WOS:000380808300016
PM 23808668
ER
PT J
AU Awuah, P
Bera, TK
Folivi, M
Chertov, O
Pastan, I
AF Awuah, Prince
Bera, Tapan K.
Folivi, Messan
Chertov, Oleg
Pastan, Ira
TI Reduced Shedding of Surface Mesothelin Improves Efficacy of
Mesothelin-Targeting Recombinant Immunotoxins
SO MOLECULAR CANCER THERAPEUTICS
LA English
DT Article
ID EXPRESSING TUMOR XENOGRAFTS; PANCREATIC-CANCER; MONOCLONAL-ANTIBODY;
POTENTIATING FACTOR; ANTITUMOR-ACTIVITY; SOLID TUMORS; IN-VITRO;
THERAPY; OVARIAN; SS1P
AB Mesothelin (MSLN) is a differentiation antigen that is highly expressed in many epithelial cancers. MSLN is an important therapeutic target due to its high expression in cancers and limited expression in normal human tissues. Although it has been assumed that shed antigen is a barrier to immunotoxin action, a modeling study predicted that shed MSLN may enhance the action of MSLN-targeting recombinant immunotoxins such as SS1P and similar therapeutics by facilitating their redistribution within tumors. We aimed to determine whether shed MSLN enhances or reduces the antitumor effect of MSLN-targeting immunotoxins SS1P and RG7787. We engineered a cell line, A431/G9 (TACE mutant) that expresses a mutant form of MSLN in which the TNF-converting enzyme protease site is replaced with GGGS. We compared the response of the TACE-mutant cells with immunotoxins SS1P and RG7787 with that of the parental A431/H9 cell line. We show that TACE-mutant cells shed 80% less MSLN than A431/H9 cells, that TACE-mutant cells show a 2- to 3-fold increase in MSLN-targeted immunotoxin uptake, and that they are about 5-fold more sensitive to SS1P killing in cell culture. Tumors with reduced shedding respond significantly better to treatment with SS1P and RG7787. Our data show that MSLN shedding is an impediment to the antitumor activity of SS1P and RG7787. Approaches that decrease MSLN shedding could enhance the efficacy of immunotoxins and immunoconjugates targeting MSLN-expressing tumors. (C) 2016 AACR.
C1 [Awuah, Prince; Bera, Tapan K.; Folivi, Messan; Pastan, Ira] NCI, Mol Biol Lab, Ctr Canc Res, NIH, Bldg 37, Bethesda, MD 20892 USA.
[Chertov, Oleg] Leidos Biomed Inc, Canc Res Technol Program, Frederick, MD USA.
RP Pastan, I (reprint author), NCI, NIH, 37 Convent Dr,Room 5106, Bethesda, MD 20892 USA.
EM pastani@mail.nih.gov
FU Intramural Research Program of the NIH, National Cancer Institute,
Center for Cancer Research; National Cancer Institute, NIH
[HHSN261200800001E]
FX This work was supported in part by the Intramural Research Program of
the NIH, National Cancer Institute, Center for Cancer Research and by
the National Cancer Institute, NIH, Contract HHSN261200800001E (to O.
Chertov).
NR 40
TC 0
Z9 0
U1 1
U2 1
PU AMER ASSOC CANCER RESEARCH
PI PHILADELPHIA
PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA
SN 1535-7163
EI 1538-8514
J9 MOL CANCER THER
JI Mol. Cancer Ther.
PD JUL
PY 2016
VL 15
IS 7
BP 1648
EP 1655
DI 10.1158/1535-7163.MCT-15-0863
PG 8
WC Oncology
SC Oncology
GA DS9FC
UT WOS:000381087200021
PM 27196771
ER
PT J
AU Gurard-Levin, ZA
Wilson, LOW
Pancaldi, V
Postel-Vinay, S
Sousa, FG
Reyes, C
Marangoni, E
Gentien, D
Valencia, A
Pommier, Y
Cottu, P
Almouzni, G
AF Gurard-Levin, Zachary A.
Wilson, Laurence O. W.
Pancaldi, Vera
Postel-Vinay, Sophie
Sousa, Fabricio G.
Reyes, Cecile
Marangoni, Elisabetta
Gentien, David
Valencia, Alfonso
Pommier, Yves
Cottu, Paul
Almouzni, Genevieve
TI Chromatin Regulators as a Guide for Cancer Treatment Choice
SO MOLECULAR CANCER THERAPEUTICS
LA English
DT Article
ID NEGATIVE BREAST-CANCER; GENE-EXPRESSION; INDUCTION CHEMOTHERAPY; RANDOM
FORESTS; CLASSIFICATION; CENTROMERES; RESISTANCE; CHAPERONES; CELLS;
EPIGENETICS
AB The limited capacity to predict a patient's response to distinct chemotherapeutic agents is a major hurdle in cancer management. The efficiency of a large fraction of current cancer therapeutics (radio-and chemotherapies) is influenced by chromatin structure. Reciprocally, alterations in chromatin organization may affect resistance mechanisms. Here, we explore how the misexpression of chromatin regulators-factors involved in the establishment and maintenance of functional chromatin domains-can inform about the extent of docetaxel response. We exploit Affymetrix and NanoString gene expression data for a set of chromatin regulators generated from breast cancer patient-derived xenograft models and patient samples treated with doc-etaxel. Random Forest classification reveals specific panels of chromatin regulators, including key components of the SWI/SNF chromatin remodeler, which readily distinguish docetaxel highresponders and poor-responders. Further exploration of SWI/SNF components in the comprehensive NCI-60 dataset reveals that the expression inversely correlates with docetaxel sensitivity. Finally, we show that loss of the SWI/SNF subunit BRG1 (SMARCA4) in a model cell line leads to enhanced docetaxel sensitivity. Altogether, our findings point toward chromatin regulators as biomarkers for drug response as well as therapeutic targets to sensitize patients toward docetaxel and combat drug resistance. (C) 2016 AACR.
C1 [Gurard-Levin, Zachary A.; Wilson, Laurence O. W.; Almouzni, Genevieve] PSL Res Univ, CNRS, Inst Curie, UMR3664,Equipe Labellisee Ligue Canc, Paris, France.
[Gurard-Levin, Zachary A.; Wilson, Laurence O. W.; Almouzni, Genevieve] Univ Paris 06, Sorbonne Univ, CNRS, UMR3664, Paris, France.
[Pancaldi, Vera; Valencia, Alfonso] Care of Fernandez M, Spanish Natl Canc Res Ctr CNIO, Madrid, Spain.
[Postel-Vinay, Sophie] Gustave Roussy, DITEP Dept Innovat Therapeut & Essais Precoces, Villejuif, France.
[Postel-Vinay, Sophie] Gustave Roussy, Inserm Unit U981, Villejuif, France.
[Postel-Vinay, Sophie] Univ Paris 11, Univ Paris Saclay, Fac Med, Le Kremlin Bicetre, France.
[Sousa, Fabricio G.; Pommier, Yves] NCI, Dev Therapeut Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
[Sousa, Fabricio G.; Pommier, Yves] NCI, Mol Pharmacol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA.
[Reyes, Cecile; Marangoni, Elisabetta; Gentien, David] PSL Res Univ, Inst Curie, Translat Res Dept, Genom Platform, Paris, France.
[Cottu, Paul] Inst Curie, Med Oncol, Paris, France.
RP Almouzni, G (reprint author), PSL Res Univ, CNRS, Inst Curie, UMR3664,Equipe Labellisee Ligue Canc, Paris, France.; Almouzni, G (reprint author), Univ Paris 06, Sorbonne Univ, CNRS, UMR3664, Paris, France.; Gurard-Levin, ZA (reprint author), Inst Curie, 26 Rue Ulm, F-75005 Paris, France.
EM zachary.gurard-levin@curie.fr; genevieve.almouzni@curie.fr
RI Sousa, Fabricio/O-8878-2015; Pancaldi, Vera/I-2555-2015
OI Sousa, Fabricio/0000-0003-0444-754X; valencia,
alfonso/0000-0002-8937-6789; Pancaldi, Vera/0000-0002-7433-624X
FU la Ligue Nationale contre le Cancer (Equipe labellisee Ligue); European
Commission Network of Excellence EpiGeneSys [HEALTH-F4-2010-257082]; ERC
[2009AdG_20090506, 678563]; European Commission [FP7_HEALTH-2010-259743,
ANR-11-LABX-0044_DEEP, ANR-10-IDEX-0001-02 PSL, ANR-12-BSV5-0022-02,
ANR-14-CE16-0009, ANR-14-CE100013]; Aviesan ITMO cancer project
"Epigenomics of breast cancer; Grant "INCa-DGOS-4654" [SIRIC11-002];
FEBS fellowship; NIH Intramural Program, Center for Cancer Research [Z01
BC 006150]; [ANR10-IDEX-0001-02 PSL]; [ANR-11-LBX-0044]
FX G. Almouzni's team is supported by la Ligue Nationale contre le Cancer
(Equipe labellisee Ligue), the European Commission Network of Excellence
EpiGeneSys [HEALTH-F4-2010-257082; an EpiGeneSys small call award to
Z.A. Gurard-Levin and V. Pancaldi (2014)] ERC Advanced Grant
2009AdG_20090506 "Eccentric," ERC Proof of Concept No 678563 "EPOCH28"
to G. Almouzni, the European Commission large-scale integrating project
FP7_HEALTH-2010-259743 "MODHEP," ANR-11-LABX-0044_DEEP and
ANR-10-IDEX-0001-02 PSL, ANR "CHAPINHIB" ANR-12-BSV5-0022-02, ANR
"Epicure" ANR-14-CE16-0009, ANR "CELLECTCHIP" ANR-14-CE100013, and
Aviesan ITMO cancer project "Epigenomics of breast cancer." Acquisition
of the Nanostring platform was initiated and supported by
ANR10-IDEX-0001-02 PSL, ANR-11-LBX-0044, and Grant "INCa-DGOS-4654"
SIRIC11-002. V. Pancaldi is supported by a FEBS fellowship. Y. Pommier
and F.G. Sousa were supported by the NIH Intramural Program, Center for
Cancer Research (Z01 BC 006150).
NR 58
TC 1
Z9 1
U1 1
U2 4
PU AMER ASSOC CANCER RESEARCH
PI PHILADELPHIA
PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA
SN 1535-7163
EI 1538-8514
J9 MOL CANCER THER
JI Mol. Cancer Ther.
PD JUL
PY 2016
VL 15
IS 7
BP 1768
EP 1777
DI 10.1158/1535-7163.MCT-15-1008
PG 10
WC Oncology
SC Oncology
GA DS9FC
UT WOS:000381087200032
PM 27196757
ER
PT J
AU Hammel, M
Amlanjyoti, D
Reyes, FE
Chen, JH
Parpana, R
Tang, HYH
Larabell, CA
Tainer, JA
Adhya, S
AF Hammel, Michal
Amlanjyoti, Dhar
Reyes, Francis E.
Chen, Jian-Hua
Parpana, Rochelle
Tang, Henry Y. H.
Larabell, Carolyn A.
Tainer, John A.
Adhya, Sankar
TI HU multimerization shift controls nucleoid compaction
SO SCIENCE ADVANCES
LA English
DT Article
ID X-RAY-SCATTERING; BINDING PROTEIN HU; ESCHERICHIA-COLI; DNA-BINDING;
BIOLOGICAL MACROMOLECULES; CRYSTAL-STRUCTURE; GENE-EXPRESSION; BENT DNA;
SAXS; TRANSCRIPTION
AB Molecular mechanisms controlling functional bacterial chromosome (nucleoid) compaction and organization are surprisingly enigmatic but partly depend on conserved, histone-like proteins HU alpha alpha and HU alpha beta and their interactions that span the nanoscale and mesoscale from protein-DNA complexes to the bacterial chromosome and nucleoid structure. We determined the crystal structures of these chromosome-associated proteins in complex with native duplex DNA. Distinct DNA binding modes of HU alpha alpha and HU alpha beta elucidate fundamental features of bacterial chromosome packing that regulate gene transcription. By combining crystal structures with solution x-ray scattering results, we determined architectures of HU-DNA nucleoproteins in solution under near-physiological conditions. These macromolecular conformations and interactions result in contraction at the cellular level based on in vivo imaging of native unlabeled nucleoid by soft x-ray tomography upon HU beta and ectopic HU alpha 38 expression. Structural characterization of charge-altered HU alpha alpha-DNA complexes reveals an HU molecular switch that is suitable for condensing nucleoid and reprogramming noninvasive Escherichia coli into an invasive form. Collective findings suggest that shifts between networking and cooperative and noncooperative DNA-dependent HU multimerization control DNA compaction and supercoiling independently of cellular topoisomerase activity. By integrating x-ray crystal structures, x-ray scattering, mutational tests, and x-ray imaging that span from protein-DNA complexes to the bacterial chromosome and nucleoid structure, we show that defined dynamic HU interaction networks can promote nucleoid reorganization and transcriptional regulation as efficient general microbial mechanisms to help synchronize genetic responses to cell cycle, changing environments, and pathogenesis.
C1 [Hammel, Michal; Reyes, Francis E.; Parpana, Rochelle; Tang, Henry Y. H.; Larabell, Carolyn A.; Tainer, John A.] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging, Berkeley, CA 94720 USA.
[Amlanjyoti, Dhar; Adhya, Sankar] NCI, Mol Biol Lab, Ctr Canc Res, NIH, Bldg 37, Bethesda, MD 20892 USA.
[Chen, Jian-Hua; Larabell, Carolyn A.] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA.
[Tainer, John A.] Univ Texas MD Anderson Canc Ctr, Dept Mol & Cellular Oncol, 1515 Holcombe Blvd, Houston, TX 77030 USA.
RP Hammel, M; Tainer, JA (reprint author), Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging, Berkeley, CA 94720 USA.; Tainer, JA (reprint author), Univ Texas MD Anderson Canc Ctr, Dept Mol & Cellular Oncol, 1515 Holcombe Blvd, Houston, TX 77030 USA.
EM mhammel@lbl.gov; jatainer@lbl.gov
FU NCI NIH HHS [P01 CA092584]; NIGMS NIH HHS [P41 GM103445, R01 GM105404]
NR 57
TC 1
Z9 1
U1 6
U2 7
PU AMER ASSOC ADVANCEMENT SCIENCE
PI WASHINGTON
PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA
SN 2375-2548
J9 SCI ADV
JI Sci. Adv.
PD JUL
PY 2016
VL 2
IS 7
AR e1600650
DI 10.1126/sciadv.1600650
PG 11
WC Multidisciplinary Sciences
SC Science & Technology - Other Topics
GA DT9GQ
UT WOS:000381805300035
PM 27482541
ER
PT J
AU Tian, PF
Jones, RW
Yu, F
AF Tian, Pengfei
Jones, Richard W.
Yu, Fei
TI Elliptical modelling of hysteresis operating characteristics in a
dielectric elastomer tubular actuator
SO SMART MATERIALS AND STRUCTURES
LA English
DT Article
DE high voltage power supply; slew-rate; hysteresis; ellipse; dielectric
elastomer; tubular actuator; linearization
ID COMPENSATION; FABRICATION; DAMPERS
AB A dielectric elastomer (DE) tubular actuator, based on compliant metal electrode technology, exhibits hysteresis-like characteristics when driven with a low power rated high voltage power supply (HVPS). This behavior occurs mainly because the DE actuator acts as a capacitive load compromising the 'slew rate' of the HVPS during the actuator's operation. The motivation of this contribution is to investigate the use of elliptical modelling approaches for capturing the hysteresis characteristics exhibited by the DE tubular actuator when it is driven by a low cost low power rated HVPS. The DE tubular actuator considered in this work demonstrates asymmetric hysteresis behaviour due to the nonlinear voltage-strain behaviour of the actuator. A linearization filter placed in series with the actuator (during its operation) ensures a symmetric hysteresis characteristic that can then be modelled using an ellipse-based approach. Elliptical models come in many forms with the two most popular being the constrained general conic form and the general parametric form. Elliptical-based hysteresis model fits are carried out on experimental data obtained from the application of periodic input voltages, at a number of different low-frequencies, to the tubular actuator. The range of frequencies used is related to the possible use of the tubular actuator for attenuating low frequency vibration during DE actuator-based load positioning applications. Constrained conic and general parametric forms of elliptical model are used for modelling the hysteresis characteristics of the DE actuator and rate dependent models developed based on both approaches. The sensitivity of both of these rate dependent models to small inaccuracies in model parameters was then investigated. The general parametric form was found to be more robust in this respect.
C1 [Tian, Pengfei; Yu, Fei] Univ Southern Denmark, Mads Clausen Inst, Alsion 2, DK-6400 Sonderborg, Denmark.
[Jones, Richard W.] Univ York, Dept Comp Sci, York YO10 5DD, N Yorkshire, England.
[Tian, Pengfei] NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA.
RP Yu, F (reprint author), Univ Southern Denmark, Mads Clausen Inst, Alsion 2, DK-6400 Sonderborg, Denmark.
EM pengfei.tian@nih.gov; richardwjones1014@hotmail.com; fei@mci.sdu.dk
NR 37
TC 0
Z9 0
U1 2
U2 5
PU IOP PUBLISHING LTD
PI BRISTOL
PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND
SN 0964-1726
EI 1361-665X
J9 SMART MATER STRUCT
JI Smart Mater. Struct.
PD JUL
PY 2016
VL 25
IS 7
AR 075038
DI 10.1088/0964-1726/25/7/075038
PG 11
WC Instruments & Instrumentation; Materials Science, Multidisciplinary
SC Instruments & Instrumentation; Materials Science
GA DT5IY
UT WOS:000381517900041
ER
PT J
AU Bachran, C
Leppla, SH
AF Bachran, Christopher
Leppla, Stephen H.
TI Tumor Targeting and Drug Delivery by Anthrax Toxin
SO TOXINS
LA English
DT Review
DE drug delivery; immunotoxin; targeted toxin; cancer; tumor therapies
ID CAPILLARY MORPHOGENESIS PROTEIN-2; THERAPY PRODUCES REGRESSIONS;
PROTECTIVE ANTIGEN PORE; LETHAL FACTOR CLEAVES; CANCER-CELL-LINES;
ATHYMIC NUDE-MICE; BACILLUS-ANTHRACIS; MAMMALIAN-CELLS; EDEMA TOXIN;
IN-VIVO
AB Anthrax toxin is a potent tripartite protein toxin from Bacillus anthracis. It is one of the two virulence factors and causes the disease anthrax. The receptor-binding component of the toxin, protective antigen, needs to be cleaved by furin-like proteases to be activated and to deliver the enzymatic moieties lethal factor and edema factor to the cytosol of cells. Alteration of the protease cleavage site allows the activation of the toxin selectively in response to the presence of tumor-associated proteases. This initial idea of re-targeting anthrax toxin to tumor cells was further elaborated in recent years and resulted in the design of many modifications of anthrax toxin, which resulted in successful tumor therapy in animal models. These modifications include the combination of different toxin variants that require activation by two different tumor-associated proteases for increased specificity of toxin activation. The anthrax toxin system has proved to be a versatile system for drug delivery of several enzymatic moieties into cells. This highly efficient delivery system has recently been further modified by introducing ubiquitin as a cytosolic cleavage site into lethal factor fusion proteins. This review article describes the latest developments in this field of tumor targeting and drug delivery.
C1 [Bachran, Christopher] BioMed X Innovat Ctr, D-69120 Heidelberg, Germany.
[Leppla, Stephen H.] NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA.
RP Bachran, C (reprint author), BioMed X Innovat Ctr, D-69120 Heidelberg, Germany.
EM bachran@bio.mx; sleppla@niaid.nih.gov
FU Intramural Research Program of the National Institute of Allergy and
Infectious Diseases (NIAID), Bethesda, MD, USA
FX This work was supported by the Intramural Research Program of the
National Institute of Allergy and Infectious Diseases (NIAID), Bethesda,
MD, USA.
NR 99
TC 1
Z9 1
U1 11
U2 11
PU MDPI AG
PI BASEL
PA ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
SN 2072-6651
J9 TOXINS
JI Toxins
PD JUL
PY 2016
VL 8
IS 7
AR 197
DI 10.3390/toxins8070197
PG 16
WC Toxicology
SC Toxicology
GA DS4PU
UT WOS:000380763800007
ER
PT J
AU Forman-Hoffman, VL
Hedden, SL
Glasheen, C
Davies, C
Colpe, LJ
AF Forman-Hoffman, Valerie L.
Hedden, Sarra L.
Glasheen, Cristie
Davies, Christine
Colpe, Lisa J.
TI The role of mental illness on cigarette dependence and successful
quitting in a nationally representative, household-based sample of US
adults
SO ANNALS OF EPIDEMIOLOGY
LA English
DT Article
DE Cigarette smoking; Tobacco use disorder; Smoking cessation; Mental
disorders
ID NONSPECIFIC PSYCHOLOGICAL DISTRESS; HEALTH INTERVIEW SURVEY;
SMOKING-CESSATION; UNITED-STATES; NICOTINE DEPENDENCE; FAGERSTROM TEST;
TOBACCO USE; PSYCHIATRIC-DISORDERS; ADDICTIVE DISORDERS; DRUG-USE
AB Purpose: To begin to explore whether the association between mental illness (MI), cigarette dependence, and unsuccessful quit attempts differs across particular demographic subgroups.
Methods: This study examines data from adults aged 18 years or older participating in the 2008-2012 National Surveys on Drug Use and Health. Analyses explored the moderating effects of age, gender, and race and/or ethnicity on associations between three levels of MI: (serious mental illness [SMI], any mental illness but no SMI, and no MI) and two smoking-related outcomes (cigarette dependence among current smokers and successful quitting among ever daily smokers).
Results: After confirming that adults with MI were more likely to be dependent on cigarettes and less likely to successfully quit smoking, particularly among those with SMI, adjusted analyses indicated that age (but not gender or race/ethnicity) moderated the associations between MI and cigarette dependence and between MI.
Conclusions: The magnitude of the association between MI and cigarette dependence and between MI and successful quitting appears to be stronger among older adults than among younger adults. Identifying subgroups at particular high risk of cigarette dependence is paramount to targeting smoking prevention, cessation, and treatment services appropriately. Published by Elsevier Inc.
C1 [Forman-Hoffman, Valerie L.; Glasheen, Cristie; Davies, Christine] RTI Int, Res Triangle Pk, NC USA.
[Hedden, Sarra L.] SAMHSA, Subst Abuse & Mental Hlth Serv Adm, Ctr Behav Hlth Stat & Qual, Rockville, MD USA.
[Colpe, Lisa J.] NIMH, Div Serv & Intervent Res, 6001 Execut Blvd, Bethesda, MD 20892 USA.
RP Colpe, LJ (reprint author), NIMH, Div Serv & Intervent Res, 6001 Execut Blvd, Bethesda, MD 20892 USA.
EM lcolpe@mail.nih.gov
OI Glasheen, Cristie/0000-0003-4419-9659
FU NIMH; SAMHSA; [HHSS283201300001 C]
FX This work was supported by NIMH and SAMHSA and funded through contract
HHSS283201300001 C.
NR 43
TC 1
Z9 1
U1 3
U2 3
PU ELSEVIER SCIENCE INC
PI NEW YORK
PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
SN 1047-2797
EI 1873-2585
J9 ANN EPIDEMIOL
JI Ann. Epidemiol.
PD JUL
PY 2016
VL 26
IS 7
BP 447
EP 454
DI 10.1016/j.annepidem.2016.05.004
PG 8
WC Public, Environmental & Occupational Health
SC Public, Environmental & Occupational Health
GA DS6BL
UT WOS:000380866600001
PM 27247163
ER
PT J
AU Dahle, O
Kuehn, MR
AF Dahle, Oyvind
Kuehn, Michael R.
TI Inhibiting Smad2/3 Signaling in Pluripotent Mouse Embryonic Stem Cells
Enhances Endoderm Formation by Increasing Transcriptional Priming of
Lineage-Specifying Target Genes
SO DEVELOPMENTAL DYNAMICS
LA English
DT Article
DE Smad2/3; nodal signaling; embryonic stem cells; pluripotency;
transcriptional priming; Prdm14; Jarid2; polycomb repressive complex 2
ID HISTONE DEMETHYLASE JMJD3; RNA-POLYMERASE-II; NAIVE PLURIPOTENCY;
DIFFERENTIATION; PRDM14; JARID2; STATE
AB Background: Pluripotent embryonic stem cells (ESCs) offer great potential for regenerative medicine. However, efficient in vitro generation of specific desired cell types is still a challenge. We previously established that Smad2/3 signaling, essential for endoderm formation, regulates target gene expression by counteracting epigenetic repression mediated by Polycomb Repressive Complex 2 (PRC2). Although this mechanism has been demonstrated during differentiation and reprogramming, little is known of its role in pluripotent cells. Results: Chromatin immunoprecipitation-deep sequencing of undifferentiated mouse ESCs inhibited for Smad2/3 signaling identified Prdm14, important for protecting pluripotency, as a target gene. Although Prdm14 accumulates the normally repressive PRC2 deposited histone modification H3K27me3 under these conditions, surprisingly, expression increases. Analysis indicates that increased H3K27me3 leads to increased binding of PRC2 accessory component Jarid2 and recruitment of RNA polymerase II. Similar increases were found at the Nodal endoderm target gene Eomes but it remained unexpressed in pluripotent cells as normal. Upon differentiation, however, Eomes expression was significantly higher than in cells that had not been inhibited for signaling before differentiation. In addition, endoderm formation was markedly increased. Conclusions: Blocking Smad2/3 signaling in pluripotent stem cells results in epigenetic changes that enhance the capacity for endoderm differentiation. (C) 2016 Wiley Periodicals, Inc.
C1 [Dahle, Oyvind; Kuehn, Michael R.] NCI, Basic Res Lab, NIH, Frederick, MD 21701 USA.
RP Kuehn, MR (reprint author), NCI, Frederick, MD 21702 USA.
EM mkuehn@mail.nih.gov
FU National Cancer Institute, National Institutes of Health
FX Grant sponsor: the Intramural Research Program of the National Cancer
Institute, National Institutes of Health.
NR 21
TC 0
Z9 0
U1 3
U2 3
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 1058-8388
EI 1097-0177
J9 DEV DYNAM
JI Dev. Dyn.
PD JUL
PY 2016
VL 245
IS 7
SI SI
BP 807
EP 815
DI 10.1002/DVDY.24407
PG 9
WC Anatomy & Morphology; Developmental Biology
SC Anatomy & Morphology; Developmental Biology
GA DT8AJ
UT WOS:000381709600011
PM 27012147
ER
PT J
AU Arbore, G
Kemper, C
AF Arbore, Giuseppina
Kemper, Claudia
TI A novel "complement-metabolism-inflammasome axis" as a key regulator of
immune cell effector function
SO EUROPEAN JOURNAL OF IMMUNOLOGY
LA English
DT Review
DE Complement; Metabolism; NLRP3 inflammasome
ID CD4(+) T-CELLS; NLRP3 INFLAMMASOME; MEDIATED INFLAMMATION;
DIABETIC-NEPHROPATHY; IL-1-BETA SECRETION; PATTERN-RECOGNITION; INNATE
IMMUNITY; HUMAN-MONOCYTES; CUTTING EDGE; IFN-GAMMA
AB The inflammasomes are intracellular multiprotein complexes that induce and regulate the generation of the key pro-inflammatory cytokines IL-1 beta and IL-18 in response to infectious microbes and cellular stress. The activation of inflammasomes involves several upstream signals including classic pattern or danger recognition systems such as the TLRs. Recently, however, the activation of complement receptors, such as the anaphylatoxin C3a and C5a receptors and the complement regulator CD46, in conjunction with the sensing of cell metabolic changes, for instance increased amino acid influx and glycolysis (via mTORC1), have emerged as additional critical activators of the inflammasome. This review summarizes recent advances in our knowledge about complement-mediated inflammasome activation, with a specific focus on a novel " complement - metabolism - NLRP3 inflammasome axis."
C1 [Arbore, Giuseppina; Kemper, Claudia] Kings Coll London, Div Transplant Immunol & Mucosal Biol, MRC Ctr Transplantat, London SE1 9RT, England.
[Kemper, Claudia] NHLBI, Lab Mol Immunol, NIH, Bldg 10, Bethesda, MD 20892 USA.
[Kemper, Claudia] NHLBI, Ctr Immunol, NIH, Bldg 10, Bethesda, MD 20892 USA.
RP Kemper, C (reprint author), Kings Coll London, Div Transplant Immunol & Mucosal Biol, MRC Ctr Transplantat, London SE1 9RT, England.
EM Claudia.kemper@kcl.ac.uk
FU MRC Centre [MR/J006742/1]; EU; Wellcome Trust Investigator Award; King's
Bioscience Institute at King's College London; National Institute for
Health Research (NIHR) Biomedical Research Centre based at Guy's and St.
Thomas' NHS Foundation Trust and King's College London; Division of
Intramural Research, National Heart, Lung, and Blood Institute, NIH;
intramural research program of NIAID, NIH
FX Work in the Kemper laboratory is supported by the MRC Centre. Grant
MR/J006742/1, an EU-funded Innovative Medicines Initiative BTCURE
(C.K.), a Wellcome Trust Investigator Award (C.K.), and the King's
Bioscience Institute at King's College London (G.A.), the National
Institute for Health Research (NIHR) Biomedical Research Centre based at
Guy's and St. Thomas' NHS Foundation Trust and King's College London,
and by the Division of Intramural Research, National Heart, Lung, and
Blood Institute, NIH and the intramural research program of NIAID, NIH.
NR 92
TC 5
Z9 5
U1 5
U2 7
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0014-2980
EI 1521-4141
J9 EUR J IMMUNOL
JI Eur. J. Immunol.
PD JUL
PY 2016
VL 46
IS 7
BP 1563
EP 1573
DI 10.1002/eji.201546131
PG 11
WC Immunology
SC Immunology
GA DS4LM
UT WOS:000380752600004
PM 27184294
ER
PT J
AU Bode, C
Fox, M
Tewary, P
Steinhagen, A
Ellerkmann, RK
Klinman, D
Baumgarten, G
Hornung, V
Steinhagen, F
AF Bode, Christian
Fox, Mario
Tewary, Poonam
Steinhagen, Almut
Ellerkmann, Richard K.
Klinman, Dennis
Baumgarten, Georg
Hornung, Veit
Steinhagen, Folkert
TI Human plasmacytoid dentritic cells elicit a Type I Interferon response
by sensing DNA via the cGAS-STING signaling pathway
SO EUROPEAN JOURNAL OF IMMUNOLOGY
LA English
DT Article
DE Cytosolic sensor; Dentritic cells; DNA; Innate immunity; Interferon;
Toll-like-receptors
ID CYCLIC GMP-AMP; DENDRITIC CELLS; CYTOSOLIC DNA; ANTIVIRAL DEFENSE;
IMMUNE-SYSTEM; IFN-BETA; RECOGNITION; 2ND-MESSENGER; ACTIVATION;
SYNTHASE
AB Plasmacytoid dendritic cells (pDCs) are a major source of type I interferon (IFN) and are important for host defense by sensing microbial DNA via TLR9. pDCs also play a critical role in the pathogenesis of IFN-driven autoimmune diseases. Yet, this autoimmune reaction is caused by the recognition of self-DNA and has been linked to TLR9-independent pathways. Increasing evidence suggests that the cytosolic DNA receptor cyclic GMP-AMP (cGAMP) synthase (cGAS) is a critical component in the detection of pathogens and contributes to autoimmune diseases. It has been shown that binding of DNA to cGAS results in the synthesis of cGAMP and the subsequent activation of the stimulator of interferon genes (STING) adaptor to induce IFNs. Our results show that the cGAS-STING pathway is expressed and activated in human pDCs by cytosolic DNA leading to a robust type I IFN response. Direct activation of STING by cyclic dinucleotides including cGAMP also activated pDCs and knockdown of STING abolished this IFN response. These results suggest that pDCs sense cytosolic DNA and cyclic dinucleotides via the cGAS-STING pathway and that targeting this pathway could be of therapeutic interest.
C1 [Bode, Christian; Fox, Mario; Ellerkmann, Richard K.; Baumgarten, Georg; Steinhagen, Folkert] Univ Bonn, Dept Anesthesiol & Crit Care Med, D-53127 Bonn, Germany.
[Tewary, Poonam] FNLCR, Expt Immunol Lab, Canc Inflammat Program, Leidos Biomed Res Inc, Frederick, MD USA.
[Klinman, Dennis] NCI, Canc & Inflammat Program, Ctr Canc Res, Frederick, MD 21701 USA.
[Steinhagen, Almut] Univ Bonn, Inst Clin Chem & Clin Pharmacol, Bonn, Germany.
[Hornung, Veit] Univ Bonn, Inst Mol Med, Bonn, Germany.
RP Bode, C; Steinhagen, F (reprint author), Univ Bonn, Dept Anesthesiol & Crit Care Med, D-53127 Bonn, Germany.
EM christian.bode@ukb.uni-bonn.de; folkert.steinhagen@ukb.uni-bonn.de
FU University of Bonn (BONFOR); National Cancer Institute, National
Institutes of Health [HHSN26120080001E]; Intramural Research Program of
the NIH, National Cancer Institute, Center for Cancer Research
FX This study was supported by grants from the University of Bonn (BONFOR).
The authors would like to thank Dr. T. Maeda and Dr. S. Kamihira
(Department of Island Medicine, Nagasaki University, Japan) for
providing CAL-1 cells. This project has been funded in whole or in part
with federal funds from the National Cancer Institute, National
Institutes of Health, under contract HHSN26120080001E. The content of
this publication does not necessarily reflect the views or policies of
the Department of Health and Human Services, nor does mention of trade
names, commercial products, or organizations imply endorsement by the
U.S. Government. This Research was supported [in part] by the Intramural
Research Program of the NIH, National Cancer Institute, Center for
Cancer Research.
NR 36
TC 2
Z9 2
U1 4
U2 5
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0014-2980
EI 1521-4141
J9 EUR J IMMUNOL
JI Eur. J. Immunol.
PD JUL
PY 2016
VL 46
IS 7
BP 1615
EP 1621
DI 10.1002/eji.201546113
PG 7
WC Immunology
SC Immunology
GA DS4LM
UT WOS:000380752600010
PM 27125983
ER
PT J
AU Kim, HK
Waickman, AT
Castro, E
Flomerfelt, FA
Hawk, NV
Kapoor, V
Telford, WG
Gress, RE
AF Kim, Hye Kyung
Waickman, Adam T.
Castro, Ehydel
Flomerfelt, Francis A.
Hawk, Nga V.
Kapoor, Veena
Telford, William G.
Gress, Ronald E.
TI Distinct IL-7 signaling in recent thymic emigrants versus mature naive T
cells controls T-cell homeostasis
SO EUROPEAN JOURNAL OF IMMUNOLOGY
LA English
DT Article
DE Apoptosis; Bcl-2; Cytokine; Proliferation; STAT5
ID IN-VIVO; CYCLE PROGRESSION; RECEPTOR-ALPHA; PROLIFERATION; EXPRESSION;
SURVIVAL; INTERLEUKIN-7; PHENOTYPE; DEATH; MICE
AB IL-7 is essential for T-cell survival but its availability is limited in vivo. Consequently, all peripheral T cells, including recent thymic emigrants (RTEs) are constantly competing for IL-7 to survive. RTEs are required to replenish TCR diversity and rejuvenate the peripheral T-cell pool. However, it remains unknown how RTEs successfully compete with resident mature T cells for IL-7. Moreover, RTEs express low levels of IL-7 receptors, presumably rendering them even less competitive. Here, we show that, surprisingly, RTEs are more responsive to IL-7 than mature naive T cells as demonstrated by markedly increased STAT5 phosphorylation upon IL-7 stimulation. Nonetheless, adoptive transfer of RTE cells into lymphopenic host mice resulted in slower IL-7-induced homeostatic proliferation and diminished expansion compared to naive donor T cells. Mechanistically, we found that IL-7 signaling in RTEs preferentially upregulated expression of Bcl-2, which is antiapoptotic but also anti-proliferative. In contrast, naive T cells showed diminished Bcl-2 induction but greater proliferative response to IL-7. Collectively, these data indicate that IL-7 responsiveness in RTE is designed to maximize survival at the expense of reduced proliferation, consistent with RTE serving as a subpopulation of T cells rich in diversity but not in frequency.
C1 [Kim, Hye Kyung; Castro, Ehydel; Flomerfelt, Francis A.; Hawk, Nga V.; Kapoor, Veena; Telford, William G.; Gress, Ronald E.] Expt Transplantat & Immunol Branch, Bethesda, MD USA.
[Waickman, Adam T.] NCI, Expt Immunol Branch, Ctr Canc Res, NIH, Bldg 10, Bethesda, MD 20892 USA.
RP Waickman, AT (reprint author), NCI, Expt Immunol Branch, Ctr Canc Res, NIH, Bldg 10, Bethesda, MD 20892 USA.; Kim, HK (reprint author), NCI, Expt Transplantat & Immunol Branch, Ctr Canc Res, NIH, Bldg 10 CRC,Room 3E-3288,10 Ctr, Bethesda, MD 20892 USA.
EM kihye@mail.nih.gov; adam.waickman@nih.gov
FU Intramural Research Program of the US National Institutes of Health;
National Cancer Institute; Center for Cancer Research
FX We thank Dr. Hyun Park for critical review of this manuscript. This
study was supported by the Intramural Research Program of the US
National Institutes of Health, the National Cancer Institute, and the
Center for Cancer Research.
NR 54
TC 1
Z9 1
U1 0
U2 0
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0014-2980
EI 1521-4141
J9 EUR J IMMUNOL
JI Eur. J. Immunol.
PD JUL
PY 2016
VL 46
IS 7
BP 1669
EP 1680
DI 10.1002/eji.201546214
PG 12
WC Immunology
SC Immunology
GA DS4LM
UT WOS:000380752600015
PM 27129922
ER
PT J
AU Burotto, M
Berkovits, A
Dunleavy, K
AF Burotto, Mauricio
Berkovits, Alejandro
Dunleavy, Kieron
TI Double hit lymphoma: from biology to therapeutic implications
SO EXPERT REVIEW OF HEMATOLOGY
LA English
DT Review
DE Double-hit; MYC; BCL2; BCL6; triple-hit; BCL2 inhibitors; MYC
inhibitors; double-expresser; DPE; DHL
ID B-CELL LYMPHOMA; RITUXIMAB PLUS CYCLOPHOSPHAMIDE; NON-HODGKINS-LYMPHOMA;
BURKITT-LYMPHOMA; C-MYC; MOLECULAR SUBTYPES; GENE-EXPRESSION; GERMINAL
CENTER; PROGNOSTIC-SIGNIFICANCE; PROTEIN EXPRESSION
AB Introduction: Diffuse large B-cell lymphoma (DLBCL) is a molecularly heterogeneous disease defined by different cellular origins and mechanisms of oncogenic activation. Approximately 10% of DLBCL cases harbor a MYC rearrangement and this has been associated with a more aggressive clinical course following standard therapy.Areas covered: So-called double-hit lymphomas' (DHL) or triple hit lymphomas' (THL) occur when MYC is concurrently rearranged with BCL2 and/or BCL6. These tumors are characterized by high proliferation rate and a very poor outcome following standard R-CHOP (rituximab, cyclophosphamide, doxorubicin vincristine and prednisone) therapy, in most (though not all) studies that have looked at this. Though there is a paucity of published experience with other chemotherapy regimens, there is emerging evidence that more intensive approaches may improve outcome. Recently, there has been a lot of focus in the literature on double-expresser lymphomas' (DEL) with high MYC, BCL2 and/or BCL6 expression but typically without rearrangements of these genes. These DEL cases, have a poor outcome with R-CHOP and there is little consensus on how they should be approached.Expert commentary: This review will focus on the biology and treatment of DHL and DEL, discuss the outcome of these diseases with current standard as well as promising new approaches and conclude with a section on novel agents that are in development for these diseases.
C1 [Burotto, Mauricio; Berkovits, Alejandro] Univ Desarrollo, Sch Med, Hemato Oncol Serv, Clin Alemana Santiago, Santiago, Chile.
[Dunleavy, Kieron] NCI, Lymphoid Malignancies Branch, Ctr Canc Res, Bethesda, MD 20892 USA.
RP Dunleavy, K (reprint author), NCI, Lymphoid Malignancy Branch, Bldg 10,Room 4N 115,9000 Rockville Pike, Bethesda, MD 20892 USA.
EM dunleavk@mail.nih.gov
FU intramural program of the National Cancer Institute
FX This work was partly supported by the intramural program of the National
Cancer Institute. The authors have no other relevant affiliations or
financial involvement with any organization or entity with a financial
interest in or financial conflict with the subject matter or materials
discussed in the manuscript apart from those disclosed.
NR 92
TC 1
Z9 1
U1 2
U2 4
PU TAYLOR & FRANCIS LTD
PI ABINGDON
PA 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND
SN 1747-4086
EI 1747-4094
J9 EXPERT REV HEMATOL
JI Expert Rev. Hematol.
PD JUL
PY 2016
VL 9
IS 7
BP 669
EP 678
DI 10.1080/17474086.2016.1182858
PG 10
WC Hematology
SC Hematology
GA DR8LQ
UT WOS:000380149800008
PM 27166590
ER
PT J
AU Chlanda, P
Zimmerberg, J
AF Chlanda, Petr
Zimmerberg, Joshua
TI Protein-lipid interactions critical to replication of the influenza A
virus
SO FEBS LETTERS
LA English
DT Review
DE influenza virus; lipid microdomains; membrane fission; membrane fusion;
virus budding; virus entry
ID HEMAGGLUTININ FUSION PEPTIDE; MEMBRANE-FUSION; M2 PROTEIN; MATRIX
PROTEIN; CHOLESTEROL-BINDING; CYTOPLASMIC TAIL; PLASMA-MEMBRANE; M1
PROTEIN; LOW PH; CRYOELECTRON TOMOGRAPHY
AB Influenza A virus (IAV) assembles on the plasma membrane where viral proteins localize to form a bud encompassing the viral genome, which ultimately pinches off to give rise to newly formed infectious virions. Upon entry, the virus faces the opposite task-fusion with the endosomal membrane and disassembly to deliver the viral genome to the cytoplasm. There are at least four influenza proteins-hemagglutinin (HA), neuraminidase (NA), matrix 1 protein (M1), and the M2 ion channel-that are known to directly interact with the cellular membrane and modify membrane curvature in order to both assemble and disassemble membrane-enveloped virions. Here, we summarize and discuss current knowledge of the interactions of lipids and membrane proteins involved in the IAV replication cycle.
C1 [Chlanda, Petr; Zimmerberg, Joshua] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Integrat Biophys, NIH, Bethesda, MD 20892 USA.
RP Zimmerberg, J (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Integrat Biophys, NIH, Bethesda, MD 20892 USA.
EM joshz@helix.nih.gov
FU Division of Intramural Research of the NICHD; Intramural Research
Program of the NIH
FX We thank to Mary L. Kraft for kindly providing the nano-SIMS figure and
Samuel T. Hess for useful suggestions. We are grateful to members of the
Zimmerberg lab and to Fredric S. Cohen for critical reading of the
manuscript. We sincerely apologize to all scientists whose important
work could not be cited here due to space restrictions. This work was
supported by the Division of Intramural Research of the NICHD, in the
Intramural Research Program of the NIH.
NR 103
TC 2
Z9 2
U1 10
U2 12
PU WILEY-BLACKWELL
PI HOBOKEN
PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
SN 0014-5793
EI 1873-3468
J9 FEBS LETT
JI FEBS Lett.
PD JUL
PY 2016
VL 590
IS 13
SU 3
SI SI
BP 1940
EP 1954
DI 10.1002/1873-3468.12118
PG 15
WC Biochemistry & Molecular Biology; Biophysics; Cell Biology
SC Biochemistry & Molecular Biology; Biophysics; Cell Biology
GA DS4OQ
UT WOS:000380760800007
PM 26921878
ER
PT J
AU Mooney, LJ
Hillhouse, MP
Thomas, C
Ang, A
Sharma, G
Terry, G
Chang, L
Walker, R
Trivedi, M
Croteau, D
Sparenborg, S
Ling, W
AF Mooney, Larissa J.
Hillhouse, Maureen P.
Thomas, Christie
Ang, Alfonso
Sharma, Gaurav
Terry, Garth
Chang, Linda
Walker, Robrina
Trivedi, Madhukar
Croteau, David
Sparenborg, Steven
Ling, Walter
TI Utilizing a Two-stage Design to Investigate the Safety and Potential
Efficacy of Monthly Naltrexone Plus Once-daily Bupropion as a Treatment
for Methamphetamine Use Disorder
SO JOURNAL OF ADDICTION MEDICINE
LA English
DT Article
DE bupropion; extended-release naltrexone; medication treatment;
methamphetamine dependence; pharmacotherapy; Vivitrol
ID PLACEBO-CONTROLLED TRIAL; DEPENDENCE; INTERVENTIONS; AMPHETAMINE;
ANTAGONIST; MANAGEMENT; BEHAVIOR; OUTCOMES
AB Objectives: This 2-stage open-label pilot study evaluated the safety and potential efficacy of naltrexone + bupropion as a pharmacotherapy for methamphetamine (MA) use disorder.
Methods: The study was conducted in 2 stages of recruitment across 3 sites; 20 participants were enrolled in stage 1 and 29 participants were enrolled in stage 2. Eight weeks of open-label pharmacotherapy with a combination of extended-release injectable naltrexone (XR-NTX; Vivitrol) plus extended-release oral bupropion (BRP; Wellbutrin XL) were provided with a smartphone-assisted medication adherence platform. Participants met Diagnostic and Statistical Manual of Mental Disorders, 5th Edition criteria for severe MA use disorder, self-reported >= 20 days of MA use in the 30 days prior to consent, and submitted 3 MA-positive urine drug screens (UDS) out of 4 collected during screening. Participants attended clinic twice weekly for observed BRP dosing, UDS testing, assessments, and medical management; XR-NTX was administered at weeks 1 and 5. A BRP taper and follow-up visit occurred in week 9.
Results: Analyses evaluated effects of XR-NTX+BRP to determine the number of "responders" according to a statistically pre-defined response criterion (6 of 8 MA-negative UDS during the last 4 weeks of medication). The 2-stage design required that stage 1 yield >= 3 responders to continue to stage 2; 11 of the 49 participants met responder criteria across both stages (5 in stage 1, 6 in stage 2).
Conclusions: Under the statistical analysis plan, study "success" required >= 9 responders. With 11 responders, the study demonstrated sufficient potential of naltrexone plus bupropion as a combination pharmacotherapy for MA use disorder to warrant further study.
C1 [Mooney, Larissa J.; Hillhouse, Maureen P.; Thomas, Christie; Ang, Alfonso; Terry, Garth; Ling, Walter] Univ Calif Los Angeles, Los Angeles, CA USA.
[Sharma, Gaurav; Croteau, David] Emmes, Rockville, MD USA.
[Chang, Linda] Univ Hawaii, Honolulu, HI 96822 USA.
[Chang, Linda] Queens Med Ctr, Honolulu, HI USA.
[Walker, Robrina; Trivedi, Madhukar] Univ Texas Southwestern Med Ctr, Dallas, TX USA.
[Sparenborg, Steven] NIDA, Bethesda, MD 20892 USA.
RP Mooney, LJ (reprint author), UCLA Integrated Subst Abuse Programs, 11075 Santa Monica Blvd,Suite 200, Los Angeles, CA 90025 USA.
EM lmooney@mednet.ucla.edu
RI Emchi, Karma/Q-1952-2016
FU National Institute on Drug Abuse [DA13045]
FX Alkermes Inc. (Waltham, MA) provided monthly naltrexone (Vivitrol).
Support for this research was provided through the National Institute on
Drug Abuse (DA13045).
NR 37
TC 0
Z9 0
U1 6
U2 6
PU LIPPINCOTT WILLIAMS & WILKINS
PI PHILADELPHIA
PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA
SN 1932-0620
EI 1935-3227
J9 J ADDICT MED
JI J. Addict. Med.
PD JUL-AUG
PY 2016
VL 10
IS 4
BP 236
EP 243
DI 10.1097/ADM.0000000000000218
PG 8
WC Substance Abuse
SC Substance Abuse
GA DS5SL
UT WOS:000380842500004
PM 27379819
ER
PT J
AU Trigo, JM
Soliman, A
Staios, G
Quilty, L
Fischer, B
George, TP
Rehm, J
Selby, P
Barnes, AJ
Huestis, MA
Le Foll, B
AF Trigo, Jose M.
Soliman, Alexandra
Staios, Gregory
Quilty, Lena
Fischer, Benedikt
George, Tony P.
Rehm, Juergen
Selby, Peter
Barnes, Allan J.
Huestis, Marilyn A.
Le Foll, Bernard
TI Sativex Associated With Behavioral-Relapse Prevention Strategy as
Treatment for Cannabis Dependence: A Case Series
SO JOURNAL OF ADDICTION MEDICINE
LA English
DT Article
DE Abstinence; cannabidiol; cannabis; marijuana; THC; withdrawal
ID MASS-SPECTROMETRY; DELTA-9-TETRAHYDROCANNABINOL; WITHDRAWAL;
QUANTIFICATION; CHROMATOGRAPHY; INDIVIDUALS; PSYCHOSIS; SEEKING; ADULTS;
THC
AB Objectives: Cannabis is the most commonly used illicit drug; a substantial minority of users develop dependence. The current lack of pharmacological treatments for cannabis dependence warrants the use of novel approaches and further investigation of promising pharmacotherapy. In this case series, we assessed the use of self-titrated dosages of Sativex (1: 1, Delta(9)-tetrahydrocannabinol [THC]/cannabidiol [CBD] combination) and motivational enhancement therapy and cognitive behavioral therapy (MET/CBT) for the treatment of cannabis dependence among 5 treatment-seeking community-recruited cannabis-dependent subjects.
Methods: Participants underwent a 3-month open-label self-titration phase with Sativex (up to 113.4 of THC/105 mg of CBD) and weekly MET/CBT, with a 3-month follow-up.
Results: Sativex was well-tolerated by all participants (average dosage 77.5 THC/71.7mg CBD). The combination of Sativex and MET/CBT reduced the amount of cannabis use and progressively reduced craving and withdrawal scores. THC/CBD metabolite concentration indicated reduced cannabis use and compliance with medication.
Conclusions: In summary, this pilot study found that with Sativex in combination with MET/CBT reduced cannabis use while preventing increases in craving and withdrawal in the 4 participants completing the study. Further systematic exploration of Sativex as a pharmacological treatment option for cannabis dependence should be performed.
C1 [Trigo, Jose M.; Soliman, Alexandra; Staios, Gregory; Le Foll, Bernard] Campbell Family Mental Hlth, Res Inst, Translat Addict Res Lab, Toronto, ON, Canada.
[Quilty, Lena] CAMH, Clin Res, Toronto, ON, Canada.
[Fischer, Benedikt; Rehm, Juergen] CAMH, Social & Epidemiol Res Dept, Toronto, ON, Canada.
[George, Tony P.] CAMH, Schizophrenia Div, Toronto, ON, Canada.
[George, Tony P.] CAMH, Complex Mental Illness Program, Toronto, ON, Canada.
[Rehm, Juergen] Univ Toronto, Addict Policy, Dalla Lana Sch Publ Hlth, Toronto, ON, Canada.
[Fischer, Benedikt; Rehm, Juergen] Univ Toronto, Inst Med Sci, Fac Med, Toronto, ON, Canada.
[Fischer, Benedikt; George, Tony P.; Rehm, Juergen; Selby, Peter] Univ Toronto, Dept Psychiat, Toronto, ON, Canada.
[Rehm, Juergen] Tech Univ Dresden, Inst Clin Psychol & Psychotherapy, Dresden, Germany.
[Rehm, Juergen] Tech Univ Dresden, Ctr Clin Epidemiol & Longitudinal Studies CELOS, Dresden, Germany.
[Selby, Peter; Le Foll, Bernard] CAMH, Addict Div, Toronto, ON, Canada.
[Selby, Peter] Univ Toronto, Dept Family & Community Med, Toronto, ON, Canada.
[Fischer, Benedikt] Simon Fraser Univ, Ctr Appl Res Mental Hlth & Addict, Fac Hlth Sci, Vancouver, BC, Canada.
[Barnes, Allan J.; Huestis, Marilyn A.] NIDA, Chem & Drug Metab, NIH, Baltimore, MD USA.
RP Le Foll, B (reprint author), CAMH, Toronto, ON, Canada.
EM bernard.lefoll@camh.ca
FU National Institute On Drug Abuse of the National Institutes of Health
[R21DA031906]; National Institute on Drug Abuse, NIH, DHHS
FX Research reported in this publication was supported by the National
Institute On Drug Abuse of the National Institutes of Health under Award
Number R21DA031906 (to Dr Le Foll) and in part by Intramural Research
Program of National Institute on Drug Abuse, NIH, DHHS.
NR 28
TC 0
Z9 0
U1 1
U2 1
PU LIPPINCOTT WILLIAMS & WILKINS
PI PHILADELPHIA
PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA
SN 1932-0620
EI 1935-3227
J9 J ADDICT MED
JI J. Addict. Med.
PD JUL-AUG
PY 2016
VL 10
IS 4
BP 274
EP 279
DI 10.1097/ADM.0000000000000229
PG 6
WC Substance Abuse
SC Substance Abuse
GA DS5SL
UT WOS:000380842500010
PM 27261670
ER
PT J
AU Flannery, M
Mohile, SG
Dale, W
Arora, NK
Azar, L
Breslau, ES
Cohen, HJ
Dotan, E
Eldadah, BA
Leach, CR
Mitchell, SA
Rowland, JH
Hurria, A
AF Flannery, Marie
Mohile, Supriya Gupta
Dale, William
Arora, Neeraj K.
Azar, Lauren
Breslau, Erica S.
Cohen, Harvey Jay
Dotan, Efrat
Eldadah, Basil A.
Leach, Corinne R.
Mitchell, Sandra A.
Rowland, Julia H.
Hurria, Arti
TI Interventions to improve the quality of life and survivorship of older
adults with cancer: The funding landscape at NIH, ACS and PCORI
SO JOURNAL OF GERIATRIC ONCOLOGY
LA English
DT Article
DE Aging; Cancer; Older adults; Frail; Funding; NIH; ACS; PCORI;
Survivorship; Quality of life
ID TRIALS
AB Identifying knowledge gaps and research opportunities in cancer and aging research was the focus of a three-part conference series led by the Cancer and Aging Research Group from 2010 to 2015. The third meeting, featured representatives from the NIA, NCI, ACS and PCORI each of whom discussed research priorities and funding opportunities in cancer and aging at their respective agencies. This manuscript reports on the proceedings of that conference with a specific focus on funding priorities for interventions to improve the quality of life and survivorship of older adults with cancer. Helpful tips from each funder regarding writing a scientifically strong research proposal are presented. (C) 2016 Elsevier Ltd. All rights reserved.
C1 [Flannery, Marie] Univ Rochester, Med Ctr, Box SON 601,Elmwood Ave, Rochester, NY 14642 USA.
[Mohile, Supriya Gupta] Univ Rochester, 601 Elmwood Ave,Box 704, Rochester, NY 14642 USA.
[Dale, William] Univ Chicago, Dept Med, Sect Geriatr & Palliat Med, MC 6098,5841 S Maryland Ave, Chicago, IL 60637 USA.
[Arora, Neeraj K.] PCORI, 1919 M St,NW,Suite 250, Washington, DC 20036 USA.
[Azar, Lauren] PCORI, 1919 M St,NW,4th Floor, Washington, DC 20036 USA.
[Breslau, Erica S.] NCI, 9609 Med Ctr Dr,3E520, Rockville, MD 20850 USA.
[Cohen, Harvey Jay] Duke Univ, Med Ctr, Room 3502 Blue Zone,Box 3003, Durham, NC 27710 USA.
[Dotan, Efrat] Fox Chase Canc Ctr, 333 Cottman Ave, Philadelphia, PA USA.
[Eldadah, Basil A.] NIA, 7201 Wisconsin Ave,3C307, Bethesda, MD 20892 USA.
[Leach, Corinne R.] ACS, 250 Williams St, Atlanta, GA 30303 USA.
[Mitchell, Sandra A.] NCI, 9609 Med Ctr Dr,3E448, Rockville, MD 20850 USA.
[Rowland, Julia H.] NCI, 9609 Med Ctr Dr,4E450, Rockville, MD 20850 USA.
[Hurria, Arti] City Hope Comprehens Canc Ctr, 1500 E Duarte Rd, Duarte, CA USA.
RP Flannery, M (reprint author), Univ Rochester, Med Ctr, Box SON 601,Elmwood Ave, Rochester, NY 14642 USA.
EM marie_flannery@urmc.rochester.edu; supriya_mohile@urmc.rochester.edu;
wdale@medicine.bsd.uchicago.edu; narora@pcori.org; lholuj@pcori.org;
breslaue@mail.nih.gov; efrat.dotan@fccc.edu; eldadahb@nia.nih.gov;
corinne.leach@cancer.org; mitchlls@mail.nih.gov; rowlandj@mail.nih.gov;
ahurria@coh.org
FU NCI NIH HHS [R25 CA102618, UG1 CA189961, R01 CA177592, UG1 CA189823];
NIA NIH HHS [U13 AG038151, R03 AG042342]
NR 11
TC 1
Z9 1
U1 0
U2 0
PU ELSEVIER SCIENCE BV
PI AMSTERDAM
PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
SN 1879-4068
EI 1879-4076
J9 J GERIATR ONCOL
JI J. Geriatr. Oncol.
PD JUL
PY 2016
VL 7
IS 4
SI SI
BP 225
EP 233
DI 10.1016/j.jgo.2016.02.001
PG 9
WC Oncology; Geriatrics & Gerontology
SC Oncology; Geriatrics & Gerontology
GA DS7NP
UT WOS:000380970500002
PM 27197917
ER
PT J
AU Berger, A
AF Berger, Ann
TI "Mother MD Intense": The Need for Intentional Healing
SO JOURNAL OF PAIN AND SYMPTOM MANAGEMENT
LA English
DT Editorial Material
C1 [Berger, Ann] NIH, Ctr Clin, 10 Ctr Dr,Bldg 10,Room 21733, Bethesda, MD 20892 USA.
RP Berger, A (reprint author), NIH, Ctr Clin, 10 Ctr Dr,Bldg 10,Room 21733, Bethesda, MD 20892 USA.
EM aberger@cc.nih.gov
NR 2
TC 0
Z9 0
U1 0
U2 0
PU ELSEVIER SCIENCE INC
PI NEW YORK
PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
SN 0885-3924
EI 1873-6513
J9 J PAIN SYMPTOM MANAG
JI J. Pain Symptom Manage.
PD JUL
PY 2016
VL 52
IS 1
BP 151
EP 153
DI 10.1016/j.jpainsymman.2016.05.006
PG 3
WC Health Care Sciences & Services; Medicine, General & Internal; Clinical
Neurology
SC Health Care Sciences & Services; General & Internal Medicine;
Neurosciences & Neurology
GA DT7QS
UT WOS:000381681900020
PM 27256617
ER
PT J
AU Nath, A
Griffin, DE
AF Nath, Avindra
Griffin, Diane E.
TI Dedication to Dr. Richard T. Johnson
SO NEUROTHERAPEUTICS
LA English
DT Biographical-Item
C1 [Nath, Avindra] NINDS, Sect Infect Nervous Syst, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
[Griffin, Diane E.] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD USA.
RP Nath, A (reprint author), NINDS, Sect Infect Nervous Syst, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
EM avindra.nath@nih.gov; dgriffi6@jhmi.edu
NR 1
TC 0
Z9 0
U1 0
U2 0
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 451
EP 452
DI 10.1007/s13311-016-0456-0
PG 2
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400001
PM 27365084
ER
PT J
AU Nath, A
Jacobson, S
AF Nath, Avindra
Jacobson, Steven
TI Current Status, Future Directions and Challenges in Management of CNS
Viral Infections
SO NEUROTHERAPEUTICS
LA English
DT Editorial Material
C1 [Jacobson, Steven] NINDS, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
RP Jacobson, S (reprint author), NINDS, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
EM avi1258@gmail.com
NR 0
TC 0
Z9 0
U1 0
U2 0
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 453
EP 454
DI 10.1007/s13311-016-0459-x
PG 2
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400002
PM 27388287
ER
PT J
AU Billioux, BJ
Smith, B
Nath, A
AF Billioux, Bridgette Jeanne
Smith, Bryan
Nath, Avindra
TI Neurological Complications of Ebola Virus Infection
SO NEUROTHERAPEUTICS
LA English
DT Review
DE Ebola; meningitis; encephalitis; microvascular disease; deafness;
vertigo; insomnia; cognition; magnetic resonance imaging
ID HEMORRHAGIC-FEVER; WEST-AFRICA; NONHUMAN-PRIMATES; SIERRA-LEONE;
DISEASE; TRANSMISSION; CONGO; MARBURG; KIKWIT; MANAGEMENT
AB Ebola virus disease is one of the deadliest pathogens known to man, with a mortality rate between 25-90% depending on the species and outbreak of Ebola. Typically, it presents with fever, headache, voluminous vomiting and diarrhea, and can progress to a hemorrhagic illness; neurologic symptoms, including meningoencephalitis, seizures, and coma, can also occur. Recently, an outbreak occurred in West Africa, affecting > 28,000 people, and killing > 11,000. Owing to the magnitude of this outbreak, and the large number (> 17,000) of Ebola survivors, the medical and scientific communities are learning much more about the acute manifestations and sequelae of Ebola. A number of neurologic complications can occur after Ebola, such as seizures, memory loss, headaches, cranial nerve abnormalities, and tremor. Ebola may also persist in some immunologically privileged sites, including the central nervous system, and can rarely lead to relapse in disease. Owing to these findings, it is important that survivors are evaluated and monitored for neurologic symptoms. Much is unknown about this disease, and treatment remains largely supportive; however, with ongoing clinical and basic science, the mechanisms of how Ebola affects the central nervous system and how it persists after acute disease will hopefully become more clear, and better treatments and clinical practices for Ebola patients will be developed.
C1 [Billioux, Bridgette Jeanne; Smith, Bryan; Nath, Avindra] NINDS, Sect Infect Nervous Syst, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
RP Nath, A (reprint author), NINDS, Sect Infect Nervous Syst, NIH, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
EM natha@ninds.nih.gov
OI Billioux, Bridgette Jeanne/0000-0003-4208-7100
NR 55
TC 0
Z9 0
U1 7
U2 8
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 461
EP 470
DI 10.1007/s13311-016-0457-z
PG 10
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400004
PM 27412684
ER
PT J
AU Anderson, MR
Kashanchi, F
Jacobson, S
AF Anderson, Monique R.
Kashanchi, Fatah
Jacobson, Steven
TI Exosomes in Viral Disease
SO NEUROTHERAPEUTICS
LA English
DT Review
DE Exosomes; microvesicles; multivesicular bodies; "back fusion"; ESCRT
ID CELL-DERIVED EXOSOMES; MATURE DENDRITIC CELLS; STRUCTURES L-PARTICLES;
HEPATITIS-C VIRUS; EXTRACELLULAR VESICLES; T-CELLS; SECRETE EXOSOMES;
EBOLA-VIRUS; DC-SIGN; PHOSPHATIDYLSERINE RECEPTOR
AB Viruses have evolved many mechanisms by which to evade and subvert the immune system to ensure survival and persistence. However, for each method undertaken by the immune system for pathogen removal, there is a counteracting mechanism utilized by pathogens. The new and emerging role of microvesicles in immune intercellular communication and function is no different. Viruses across many different families have evolved to insert viral components in exosomes, a subtype of microvesicle, with many varying downstream effects. When assessed cumulatively, viral antigens in exosomes increase persistence through cloaking viral genomes, decoying the immune system, and even by increasing viral infection in uninfected cells. Exosomes therefore represent a source of viral antigen that can be used as a biomarker for disease and targeted for therapy in the control and eradication of these disorders. With the rise in the persistence of new and reemerging viruses like Ebola and Zika, exploring the role of exosomes become more important than ever.
C1 [Anderson, Monique R.; Jacobson, Steven] NINDS, NIH, Neuroimmunol Branch, Viral Immunol Sect, Bethesda, MD 20892 USA.
[Anderson, Monique R.] Univ Virginia, Sch Med, Dept Pathol, Mol & Cellular Basis Dis Grad Program, Charlottesville, VA 22903 USA.
[Kashanchi, Fatah] George Mason Univ, Natl Ctr Biodef & Infect Dis, Mol Virol Lab, Manassas, VA 20110 USA.
RP Anderson, MR (reprint author), NINDS, NIH, Neuroimmunol Branch, Viral Immunol Sect, Bethesda, MD 20892 USA.; Anderson, MR (reprint author), Univ Virginia, Sch Med, Dept Pathol, Mol & Cellular Basis Dis Grad Program, Charlottesville, VA 22903 USA.
EM andersonmr2@mail.nih.gov
NR 149
TC 1
Z9 1
U1 7
U2 7
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 535
EP 546
DI 10.1007/s13311-016-0450-6
PG 12
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400010
PM 27324390
ER
PT J
AU Azodi, S
Jacobson, S
AF Azodi, Shila
Jacobson, Steven
TI Cytokine Therapies in Neurological Disease
SO NEUROTHERAPEUTICS
LA English
DT Review
DE Cytokine; anticytokine; neurology; drug development
ID AMYOTROPHIC-LATERAL-SCLEROSIS; MYELOPATHY/TROPICAL SPASTIC PARAPARESIS;
REMITTING MULTIPLE-SCLEROSIS; COLONY-STIMULATING FACTOR; MESSENGER-RNA
EXPRESSION; DOUBLE-BLIND; NEUROMYELITIS-OPTICA; ALZHEIMERS-DISEASE;
CEREBROSPINAL-FLUID; INFLAMMATORY MYOPATHIES
AB Cytokines are a heterogeneous group of glycoproteins that coordinate physiological functions. Cytokine deregulation is observed in many neurological diseases. This article reviews current research focused on human clinical trials of cytokine and anticytokine therapies in the treatment of several neurological disease including stroke, neuromuscular diseases, neuroinfectious diseases, demyelinating diseases, and neurobehavioral diseases. This research suggests that cytokine therapy applications may play an important role in offering new strategies for disease modulation and treatment. Further, this research provides insights into the causal link between cytokine deregulation and neurological diseases.
C1 [Azodi, Shila; Jacobson, Steven] NINDS, Viral Immunol Sect, NIH, Bethesda, MD 20892 USA.
RP Jacobson, S (reprint author), NINDS, Viral Immunol Sect, NIH, Bethesda, MD 20892 USA.
EM jacobsons@ninds.nih.gov
NR 57
TC 1
Z9 1
U1 3
U2 3
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 555
EP 561
DI 10.1007/s13311-016-0455-1
PG 7
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400012
PM 27388288
ER
PT J
AU Leibovitch, EC
Jacobson, S
AF Leibovitch, Emily C.
Jacobson, Steven
TI Vaccinations for Neuroinfectious Disease: A Global Health Priority
SO NEUROTHERAPEUTICS
LA English
DT Review
DE Vaccines; Neuroinfectious; Neuroinvasive; Meningitis; Encephalitis; CNS
infections
ID CENTRAL-NERVOUS-SYSTEM; TICK-BORNE ENCEPHALITIS; VENEZUELAN EQUINE
ENCEPHALITIS; JAPANESE ENCEPHALITIS; VIRUS-INFECTION; VACCINE STRAIN;
DENGUE VACCINE; ZIKA VIRUS; COMPLICATIONS; MICE
AB Vaccines for neuroinfectious diseases are becoming an ever-increasing global health priority, as neurologic manifestations and sequelae from existing and emerging central nervous system infections account for significant worldwide morbidity and mortality. The prevention of neurotropic infections can be achieved through globally coordinated vaccination campaigns, which have successfully eradicated nonzoonotic agents such as the variola viruses and, hopefully soon, poliovirus. This review discusses vaccines that are currently available or under development for zoonotic flaviviruses and alphaviruses, including Japanese and tick-borne encephalitis, yellow fever, West Nile, dengue, Zika, encephalitic equine viruses, and chikungunya. Also discussed are nonzoonotic agents, including measles and human herpesviruses, as well as select bacterial, fungal, and protozoal pathogens. While therapeutic vaccines will be required to treat a multitude of ongoing infections of the nervous system, the ideal vaccination strategy is pre-exposure vaccination, with the ultimate goals of minimizing disease associated with zoonotic viruses and the total eradication of nonzoonotic agents.
C1 [Leibovitch, Emily C.; Jacobson, Steven] NINDS, Viral Immunol Sect, NIH, Bethesda, MD 20892 USA.
[Leibovitch, Emily C.] George Washington Univ, Sch Med, Inst Biomed Sci, Washington, DC 20037 USA.
RP Jacobson, S (reprint author), NINDS, Viral Immunol Sect, NIH, Bethesda, MD 20892 USA.
EM jacobsons@ninds.nih.gov
NR 74
TC 1
Z9 1
U1 55
U2 55
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 562
EP 570
DI 10.1007/s13311-016-0453-3
PG 9
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400013
PM 27365085
ER
PT J
AU Haubenberger, D
Clifford, DB
AF Haubenberger, Dietrich
Clifford, David B.
TI Clinical Trials in Neurovirology: Successes, Challenges, and Pitfalls
SO NEUROTHERAPEUTICS
LA English
DT Review
DE Clinical trials; randomization; methodology; statistics; ethics
ID PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY; HERPES-SIMPLEX ENCEPHALITIS;
AIDS DEMENTIA COMPLEX; ACQUIRED-IMMUNODEFICIENCY-SYNDROME; HIV-INFECTED
INDIVIDUALS; PLACEBO-CONTROLLED TRIAL; CENTRAL-NERVOUS-SYSTEM;
COMBINATION ANTIRETROVIRAL THERAPY; POLYMERASE-CHAIN-REACTION;
CEREBROSPINAL-FLUID
AB Clinical trials in neurovirology illustrate the special challenges confronting investigators planning to study these conditions, as well as the contributions of successful trials in establishing appropriate management for these devastating diseases. This article reviews key examples of progress in neurovirology that have been spurred by clinical trials, emphasizing human herpes virus encephalitis, HIV, and JC virus. Clinical trials in the setting of neurovirological diseases are characterized by specific challenges, which may include small sample sizes, clinical presentations from life-threatening conditions to chronic courses of disease, regional and temporally restricted outbreaks scenarios, and the unavailability of validated diagnostic tests that can be rapidly deployed at the bedside. This review aims to highlight these methodological challenges and pitfalls in designing and executing clinical neurovirology trials, as well as to outline innovative trial designs, which could be useful in addressing common challenges.
C1 [Haubenberger, Dietrich] NINDS, Clin Trials Unit, Off Clin Director, Intramural Res Program,NIH, 9000 Rockville Pike,Rm 6-5700, Bethesda, MD 20892 USA.
[Clifford, David B.] Washington Univ, Dept Neurol, Box 8111,660 S Euclid Ave, St Louis, MO 63110 USA.
[Clifford, David B.] Washington Univ, Dept Med, Box 8111,660 S Euclid Ave, St Louis, MO 63110 USA.
RP Haubenberger, D (reprint author), NINDS, Clin Trials Unit, Off Clin Director, Intramural Res Program,NIH, 9000 Rockville Pike,Rm 6-5700, Bethesda, MD 20892 USA.
EM dietrich.haubenberger@nih.gov
NR 82
TC 0
Z9 0
U1 1
U2 1
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 571
EP 581
DI 10.1007/s13311-016-0440-8
PG 11
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400014
PM 27194073
ER
PT J
AU Sibley, DR
Free, RB
Xiao, JB
Ferrer, M
Southall, N
Stanwood, G
Mach, R
Bertz, J
Woods, JH
Shi, L
Marugan, JJ
AF Sibley, David R.
Free, R. Benjamin
Xiao, Jingbo
Ferrer, Marc
Southall, Noel
Stanwood, Gregg
Mach, Robert
Bertz, Jeremiah
Woods, James H.
Shi, Lei
Marugan, Juan J.
TI Identification and Characterization of a Novel and Highly Selective D-2
Dopamine Receptor Antagonist
SO NEUROTHERAPEUTICS
LA English
DT Meeting Abstract
C1 [Sibley, David R.; Free, R. Benjamin] NINDS, Mol Neuropharmacol Sect, Bldg 36,Rm 4D04, Bethesda, MD 20892 USA.
[Xiao, Jingbo; Ferrer, Marc; Southall, Noel; Marugan, Juan J.] NIH, Natl Ctr Adv Translat Sci, Rockville, MD USA.
[Stanwood, Gregg] Florida State Univ, Coll Med, Tallahassee, FL 32306 USA.
[Mach, Robert] Univ Penn, Sch Med, Philadelphia, PA 19104 USA.
[Bertz, Jeremiah; Woods, James H.] Univ Michigan, Sch Med, Ann Arbor, MI USA.
[Shi, Lei] NIDA, Baltimore, MD USA.
NR 0
TC 0
Z9 0
U1 2
U2 2
PU SPRINGER
PI NEW YORK
PA 233 SPRING ST, NEW YORK, NY 10013 USA
SN 1933-7213
EI 1878-7479
J9 NEUROTHERAPEUTICS
JI Neurotherapeutics
PD JUL
PY 2016
VL 13
IS 3
BP 657
EP 657
PG 1
WC Clinical Neurology; Neurosciences; Pharmacology & Pharmacy
SC Neurosciences & Neurology; Pharmacology & Pharmacy
GA DS3JP
UT WOS:000380679400044
ER
PT J
AU Nalbantoglu, S
Abu-Asab, M
Tan, M
Zhang, XM
Cai, L
Amri, H
AF Nalbantoglu, Sinem
Abu-Asab, Mones
Tan, Ming
Zhang, Xuemin
Cai, Ling
Amri, Hakima
TI Study of Clinical Survival and Gene Expression in a Sample of Pancreatic
Ductal Adenocarcinoma by Parsimony Phylogenetic Analysis
SO OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY
LA English
DT Article
ID GROWTH-FACTOR RECEPTOR; PHASE-III TRIAL; NATIONAL-CANCER-INSTITUTE;
POOR-PROGNOSIS; K-RAS; KRAS MUTATIONS; GEMCITABINE; ERLOTINIB; PATHWAY;
EVOLUTIONARY
AB Pancreatic ductal adenocarcinoma (PDAC) is one of the rapidly growing forms of pancreatic cancer with a poor prognosis and less than 5% 5-year survival rate. In this study, we characterized the genetic signatures and signaling pathways related to survival from PDAC, using a parsimony phylogenetic algorithm. We applied the parsimony phylogenetic algorithm to analyze the publicly available whole-genome in silico array analysis of a gene expression data set in 25 early-stage human PDAC specimens. We explain here that the parsimony phylogenetics is an evolutionary analytical method that offers important promise to uncover clonal (driver) and nonclonal (passenger) aberrations in complex diseases. In our analysis, parsimony and statistical analyses did not identify significant correlations between survival times and gene expression values. Thus, the survival rankings did not appear to be significantly different between patients for any specific gene (p > 0.05). Also, we did not find correlation between gene expression data and tumor stage in the present data set. While the present analysis was unable to identify in this relatively small sample of patients a molecular signature associated with pancreatic cancer prognosis, we suggest that future research and analyses with the parsimony phylogenetic algorithm in larger patient samples are worthwhile, given the devastating nature of pancreatic cancer and its early diagnosis, and the need for novel data analytic approaches. The future research practices might want to place greater emphasis on phylogenetics as one of the analytical paradigms, as our findings presented here are on the cusp of this shift, especially in the current era of Big Data and innovation policies advocating for greater data sharing and reanalysis.
C1 [Nalbantoglu, Sinem; Amri, Hakima] Georgetown Univ, Sch Med, Dept Biochem Cellular & Mol Biol, Washington, DC 20007 USA.
[Abu-Asab, Mones] NEI, Immunol Lab, Immunopathol Sect, Bldg 10, Bethesda, MD 20892 USA.
[Tan, Ming; Zhang, Xuemin; Cai, Ling] Georgetown Univ, Dept Biostat Bioinformat & Biomath, Washington, DC USA.
RP Amri, H (reprint author), Georgetown Univ, Sch Med, Dept Biochem Cellular & Mol Biol, Washington, DC 20007 USA.
EM amrih@georgetown.edu
FU Scientific and Technological Research Council of Turkey
FX S.N. was funded by a grant from the Scientific and Technological
Research Council of Turkey.
NR 52
TC 0
Z9 0
U1 5
U2 5
PU MARY ANN LIEBERT, INC
PI NEW ROCHELLE
PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA
SN 1536-2310
EI 1557-8100
J9 OMICS
JI OMICS
PD JUL
PY 2016
VL 20
IS 7
BP 442
EP 447
DI 10.1089/omi.2016.0059
PG 6
WC Biotechnology & Applied Microbiology; Genetics & Heredity
SC Biotechnology & Applied Microbiology; Genetics & Heredity
GA DS2NF
UT WOS:000380615000006
PM 27428255
ER
PT J
AU O'Hayre, M
Inoue, A
Kufareva, I
Wang, Z
Mikelis, CM
Drummond, RA
Avino, S
Finkel, K
Kalim, KW
DiPasquale, G
Guo, F
Aoki, J
Zheng, Y
Lionakis, MS
Molinolo, AA
Gutkind, JS
AF O'Hayre, M.
Inoue, A.
Kufareva, I.
Wang, Z.
Mikelis, C. M.
Drummond, R. A.
Avino, S.
Finkel, K.
Kalim, K. W.
DiPasquale, G.
Guo, F.
Aoki, J.
Zheng, Y.
Lionakis, M. S.
Molinolo, A. A.
Gutkind, J. S.
TI Inactivating mutations in GNA13 and RHOA in Burkitt's lymphoma and
diffuse large B-cell lymphoma: a tumor suppressor function for the G
alpha(13)/RhoA axis in B cells
SO ONCOGENE
LA English
DT Article
ID PROTEIN-COUPLED RECEPTORS; HETEROTRIMERIC G-PROTEINS; UVEAL MELANOMA;
CANCER-CELLS; SOMATIC MUTATIONS; ALPHA-SUBUNIT; P115 RHOGEF; G12 FAMILY;
ACTIVATION; METASTASIS
AB G proteins and their cognate G protein-coupled receptors (GPCRs) function as critical signal transduction molecules that regulate cell survival, proliferation, motility and differentiation. The aberrant expression and/or function of these molecules have been linked to the growth, progression and metastasis of various cancers. As such, the analysis of mutations in the genes encoding GPCRs, G proteins and their downstream targets provides important clues regarding how these signaling cascades contribute to malignancy. Recent genome-wide sequencing efforts have unveiled the presence of frequent mutations in GNA13, the gene encoding the G protein G alpha(13), in Burkitt's lymphoma and diffuse large B-cell lymphoma (DLBCL). We found that mutations in the downstream target of G alpha(13), RhoA, are also present in Burkitt's lymphoma and DLBCL. By multiple complementary approaches, we now show that that these cancer-specific GNA13 and RHOA mutations are inhibitory in nature, and that the expression of wild-type G alpha(13) in B-cell lymphoma cells with mutant GNA13 has limited impact in vitro but results in a remarkable growth inhibition in vivo. Thus, although G alpha(13) and RhoA activity has previously been linked to cellular transformation and metastatic potential of epithelial cancers, our findings support a tumor suppressive role for G alpha(13) and RhoA in Burkitt's lymphoma and DLBCL.
C1 [O'Hayre, M.; Wang, Z.; Avino, S.; Finkel, K.; Molinolo, A. A.; Gutkind, J. S.] Natl Inst Dent & Craniofacial Res, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD USA.
[Inoue, A.; Aoki, J.] Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai, Miyagi, Japan.
[Inoue, A.] Japan Sci & Technol Agcy JST, PRESTO, Kawaguchi, Saitama, Japan.
[Kufareva, I.] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA.
[Mikelis, C. M.] Texas Tech Univ, Hlth Sci Ctr, Sch Pharm, Dept Biomed Sci, Amarillo, TX USA.
[Drummond, R. A.; Lionakis, M. S.] NIAID, Fungal Pathogenesis Unit, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA.
[Avino, S.] Univ Calabria, Dept Pharm Hlth & Nutr Sci, Arcavacata Di Rende, Cs, Italy.
[Kalim, K. W.; Guo, F.; Zheng, Y.] Cincinnati Childrens Hosp Med Ctr, Div Expt Hematol & Canc Biol, Cincinnati, OH 45229 USA.
[DiPasquale, G.] Natl Inst Dent & Craniofacial Res, Mol Physiol & Therapeut Branch, NIH, Bethesda, MD USA.
[Aoki, J.] Japan Agcy Med Res & Dev, Core Res Evolut Sci & Technol AMED CREST, AMED, Chiyoda Ku, Tokyo, Japan.
[Gutkind, J. S.] UC San Diego Moores Canc Ctr, Dept Pharmacol, 3855 Hlth Sci Dr,0803, La Jolla, CA 92093 USA.
RP Gutkind, JS (reprint author), UC San Diego Moores Canc Ctr, Dept Pharmacol, 3855 Hlth Sci Dr,0803, La Jolla, CA 92093 USA.
EM sgutkind@ucsd.edu
FU National Institute of Dental and Craniofacial Research intramural
program at NIH; PRESTO from JST; AMED-CREST from AMED; Division of
Intramural Research, NIAID, NIH; NIH [R01 GM071872, R01 AI118985]
FX This study was supported by the National Institute of Dental and
Craniofacial Research intramural program at NIH. We thank Maria S Degese
for her help with immunohistochemistry. AI was funded by PRESTO from
JST. JA was funded by AMED-CREST from AMED. We thank Miho Morikawa for
technical assistance with the TGF alpha shedding assay. RAD and MSL are
supported by the Division of Intramural Research, NIAID, NIH. IK is
supported by NIH grants R01 GM071872 and R01 AI118985.
NR 47
TC 1
Z9 1
U1 3
U2 3
PU NATURE PUBLISHING GROUP
PI LONDON
PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
SN 0950-9232
EI 1476-5594
J9 ONCOGENE
JI Oncogene
PD JUL
PY 2016
VL 35
IS 29
BP 3771
EP 3780
DI 10.1038/onc.2015.442
PG 10
WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics &
Heredity
SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics &
Heredity
GA DS4LS
UT WOS:000380753200003
PM 26616858
ER
PT J
AU Ringeisen, H
Miller, S
Munoz, B
Rohloff, H
Hedden, SL
Colpe, LJ
AF Ringeisen, Heather
Miller, Shari
Munoz, Breda
Rohloff, Harley
Hedden, Sarra L.
Colpe, Lisa J.
TI Mental Health Service Use in Adolescence: Findings From the National
Survey on Drug Use and Health
SO PSYCHIATRIC SERVICES
LA English
DT Article
ID TRANSITION-AGE YOUTH; COMORBIDITY SURVEY-ADOLESCENT; YOUNG-ADULTS;
DISORDERS; PREVALENCE; CHILDREN
AB Objective: This study examined mental health service use, by service type, of adolescents ages 12-17.
Methods: Data were from approximately 113,000 adolescents who participated in the 2008-2012 National Survey on Drug Use and Health, an annual nationally representative survey of the civilian, noninstitutionalized U.S. population. Polynomial contrasts tested for linear and quadratic changes across age in the use of three types of past-year mental health services: school-based services, outpatient therapist or clinic, and overnight hospital stay.
Results: Although mental health service use increased from age 12 to age 14 across all service types, it decreased or stabilized from age 15 to 17. School-based services were the most commonly used servi