FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Kerepesi, LA Nolan, TJ Schad, GA Lustigman, S Herbert, DR Keiser, PB Nutman, TB Krolewiecki, AJ Abraham, D AF Kerepesi, LA Nolan, TJ Schad, GA Lustigman, S Herbert, DR Keiser, PB Nutman, TB Krolewiecki, AJ Abraham, D TI Human immunoglobulin G mediates protective immunity and identifies protective antigens against larval Strongyloides stercoralis in mice SO JOURNAL OF INFECTIOUS DISEASES LA English DT Article; Proceedings Paper CT 51st Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene CY NOV 10-14, 2002 CL DENVER, CO SP Amer Soc Trop Med Hyg ID VIRUS TYPE-1 INFECTION; 3RD STAGE LARVAE; BALB/CBYJ MICE; SCHISTOSOMA-MANSONI; FRACTIONATED SERA; RESPONSES; HYPERINFECTION; ANTIBODY; EOSINOPHILIA; COMPLEMENT AB Protective immunity to larval Strongyloides stercoralis in mice has been shown to be dependent on antibody, complement, and granulocytes. The goals of the present study was to determine the following: ( 1) whether human serum could passively transfer immunity to mice, ( 2) the mechanism by which the serum mediated killing, and (3) whether the antigens (Ags) recognized by the protective human antibody could induce protective immunity in mice. Immunoglobulin G (IgG) from a S. stercoralis - seropositive individual passively transferred immunity to mice. The antibody required granulocytes, but not eosinophils, and complement activation to kill the larvae. Antibody-dependent cellular cytotoxicity was not required for larval killing. Immunization of mice with soluble larval Ags isolated by use of the protective immune IgG resulted in protective immunity. In conclusion, immunity could be transferred to mice by IgG from immune humans, and Ags identified by the immune human IgG induced protective immunity in mice, which thereby suggests their possible use in a vaccine against this infection. C1 Thomas Jefferson Univ, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA. Univ Penn, Sch Vet Med, Dept Pathobiol, Philadelphia, PA 19104 USA. NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10021 USA. RP Abraham, D (reprint author), Thomas Jefferson Univ, Dept Microbiol & Immunol, 233 S 10th St, Philadelphia, PA 19107 USA. EM david.abraham@jefferson.edu RI Nolan, Thomas/A-1053-2007; Herbert, De'Broski/J-7357-2012; OI Herbert, De'Broski/0000-0002-2449-5365; Nolan, Thomas/0000-0002-6860-7947 FU NIAID NIH HHS [AI 22662, AI 47189] NR 44 TC 18 Z9 20 U1 0 U2 1 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 0022-1899 J9 J INFECT DIS JI J. Infect. Dis. PD APR 1 PY 2004 VL 189 IS 7 BP 1282 EP 1290 DI 10.1086/382484 PG 9 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 805AC UT WOS:000220338200020 PM 15031798 ER PT J AU Lu, SY Chin, FT McCarron, JA Pike, VW AF Lu, SY Chin, FT McCarron, JA Pike, VW TI Efficient O- and N-(beta-fluoroethylation)s with NCA [F-18]beta-fluoroethyl tosylate under microwave-enhanced conditions SO JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS LA English DT Article DE fluorine-18; [F-18]beta-fluoroethylation; amine; phenol; ester; pipecolinic acid; microwave ID IN-VIVO; AGENTS; BROMIDE; LIGAND AB Reactions of no-carrier-added (NCA) [F-18]beta-fluoroethyl tosylate with amine, phenol or carboxylic acid to form the corresponding [F-18]N-(beta-fluoroethyl)amine, [F-18]beta-fluoroethyl ether or [F-18]beta-fluoroethyl ester, were found to be rapid (2-10min) and efficient (51-89% conversion) under microwave-enhanced conditions. These conditions allow reactants to be heated rapidly to 150degreesC in a low boiling point solvent, such as acetonitrile, and avoid the need to use high boiling point solvents, such as DMSO and DMF, to promote reaction. The microwave-enhanced reactions gave about 20% greater radiochemical yields than thermal reactions performed at similar temperatures and over similar reaction times. With a bi-functional molecule, such as (DL)-pipecolinic acid, [F-18]beta-fluoroethyl tosylate reacts exclusively with the amino group. Copyright (C) 2004 John Wiley Sons, Ltd. C1 NIMH, Mol Imaging Branch, NIH, Bethesda, MD 20892 USA. RP Lu, SY (reprint author), NIMH, Mol Imaging Branch, NIH, Bldg 10,Room B3 C346,10 Ctr Dr, Bethesda, MD 20892 USA. EM shuiyu.lu@mail.nih.gov OI Lu, Shuiyu/0000-0003-0310-4318 NR 31 TC 16 Z9 18 U1 0 U2 5 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0362-4803 J9 J LABELLED COMPD RAD JI J. Label. Compd. Radiopharm. PD APR PY 2004 VL 47 IS 5 BP 289 EP 297 DI 10.1002/jlcr.818 PG 9 WC Biochemical Research Methods; Chemistry, Medicinal; Chemistry, Analytical SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry GA 817LM UT WOS:000221179000001 ER PT J AU Ding, K Gronenborn, AM AF Ding, K Gronenborn, AM TI Sensitivity-enhanced IPAP experiments for measuring one-bond C-13 '-C-13(alpha) and C-13(alpha)-H-1(alpha) residual dipolar couplings in proteins SO JOURNAL OF MAGNETIC RESONANCE LA English DT Article DE residual dipolar couplings; IPAP; sensitivity enhancement; proteins ID LIQUID-CRYSTALLINE PHASE; NMR-SPECTROSCOPY; IMPROVEMENT; N-15-H-1(N); N-15-C-13'; DYNAMICS; TROSY; C-13; N-15 AB Sensitivity-enhanced 2D IPAP experiments using the accordion principle for measuring one-bond C-13'-Co-13(alpha) and H-1(alpha) - C-13(alpha) dipolar couplings in proteins are presented. The resolution of the resulting spectra is identical to that of the decoupled HSQC spectra and the sensitivity of the corresponding 1D acquisitions are only slightly lower than those obtained with 3D HNCO and 3D HN(COCA)HA pulse sequences due to an additional delay 2Delta. For cases of limited resolution in the 2D H-15-N-1(N) HSQC spectrum the current pulse sequences can easily be modified into 3D versions by introducing a poorly digitized third dimension, if so desired. The experiments described here are a valuable addition to the suites available for determination of residual dipolar couplings in biological systems. (C) 2004 Elsevier Inc. All rights reserved. C1 NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Gronenborn, AM (reprint author), NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. EM gronenborn@nih.gov OI Gronenborn, Angela M/0000-0001-9072-3525 NR 22 TC 17 Z9 17 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1090-7807 J9 J MAGN RESON JI J. Magn. Reson. PD APR PY 2004 VL 167 IS 2 BP 253 EP 258 DI 10.1016/j.jmr.2003.12.016 PG 6 WC Biochemical Research Methods; Physics, Atomic, Molecular & Chemical; Spectroscopy SC Biochemistry & Molecular Biology; Physics; Spectroscopy GA 809KK UT WOS:000220635400008 PM 15040980 ER PT J AU Salomon, DS Lewis, MT AF Salomon, DS Lewis, MT TI Embryogenesis and oncogenesis: Dr Jekyll and Mr Hyde SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Editorial Material C1 NCI, Mammary Biol & Tumorigenesis Lab, NIH, Bethesda, MD 20892 USA. Baylor Coll Med, Houston, TX 77030 USA. RP Salomon, DS (reprint author), NCI, Mammary Biol & Tumorigenesis Lab, NIH, 10 Ctr Dr Bldg 10 5B39, Bethesda, MD 20892 USA. EM davetgfa@helix.nih.gov RI Lewis, Michael/A-9572-2009 NR 0 TC 1 Z9 1 U1 0 U2 0 PU KLUWER ACADEMIC/PLENUM PUBL PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD APR PY 2004 VL 9 IS 2 BP 105 EP 107 DI 10.1023/B:JOMG.0000037155.93520.4a PG 3 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA 858NY UT WOS:000224200200001 PM 15300006 ER PT J AU Callahan, R Egan, SE AF Callahan, R Egan, SE TI Notch signaling in mammary development and oncogenesis SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Review DE Notch; mammary development; breast cancer; Int3 ID ELEGANS VULVAL DEVELOPMENT; EPIDERMAL-GROWTH-FACTOR; EPITHELIAL-CELL LINE; E3 UBIQUITIN LIGASE; TRUNCATED INT3 GENE; EGF-LIKE REPEATS; F-BOX PROTEIN; RBP-J-KAPPA; C-ELEGANS; INTRACELLULAR DOMAIN AB With the discovery of an activated Notch oncogene as a causative agent in mouse mammary tumor virus induced breast cancer in mice, the potential role for Notch signaling in normal and pathological mammary development was revealed. Subsequently, Notch receptors have been found to regulate normal development in many organ systems. In addition, inappropriate Notch signaling has been implicated in cancer of several tissues in humans and animal model systems. Here we review important features of the Notch system, and how it may regulate development and cancer in the mammary gland. A large body of literature from studies in Drosophila and C elegans has not only revealed molecular details of how the Notch proteins signal to control biology, but shown that Notch receptor activation helps to define how other signaling pathways are interpreted. In many ways the Notch system is used to define the context in which other pathways function to control proliferation, differentiation, cell survival, branching morphogenesis, asymmetric cell division, and angiogenesis-all processes which are critical for normal development and function of the mammary gland. C1 NCI, Mammary Biol & Tumorigenesis Lab, Bethesda, MD 20892 USA. Hosp Sick Children, Program Dev Biol, Toronto, ON M5G 1X8, Canada. Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada. RP Callahan, R (reprint author), NCI, Mammary Biol & Tumorigenesis Lab, Bethesda, MD 20892 USA. EM rc54d@nih.gov; segan@sickkids.com NR 159 TC 104 Z9 112 U1 0 U2 5 PU KLUWER ACADEMIC/PLENUM PUBL PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD APR PY 2004 VL 9 IS 2 BP 145 EP 163 DI 10.1023/B:JOMG.0000037159.63644.81 PG 19 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA 858NY UT WOS:000224200200005 PM 15300010 ER PT J AU Satoh, K Ginsburg, E Vonderhaar, BK AF Satoh, K Ginsburg, E Vonderhaar, BK TI Msx-1 and Msx-2 in mammary gland development SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Article DE homeobox genes; Msx1; Msx2; mammary development; branching morphogenesis; progesterone ID HUMAN BREAST-CANCER; ESTROGEN-RECEPTOR STATUS; PROGESTERONE-RECEPTOR; HOMEOBOX GENE; CYCLIN D1; EPITHELIAL-CELLS; TRANSGENIC MICE; EMBRYONIC-DEVELOPMENT; HOMEODOMAIN PROTEINS; TRANSCRIPTION FACTOR AB Homeobox genes do not generally function alone to determine cell fate and morphogenesis. Rather it is the distinct combination of various members of the homeobox family of genes and their spatiotemporal patterns of expression that determine cell identity and function. Functional redundancy often makes it difficult to clearly discern the role of any one given homeobox gene. The roles that Msx1 and Msx2 play in branching morphogenesis of the mammary gland are only now becoming more evident. Many signaling pathways and transcription factors are implicated in how these homeobox genes correctly determine the morphological development of the gland. Overexpression of Msx1 and Msx2 may also be involved in tumorigenesis. Additional studies are needed to elucidate the roles of these genes in both breast development and cancer. C1 NCI, Mol & Cellular Endocrinol Sect, Mammary Biol & Tumorigenesis Lab, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. RP Vonderhaar, BK (reprint author), NCI, Mol & Cellular Endocrinol Sect, Mammary Biol & Tumorigenesis Lab, Ctr Canc Res,NIH, Bldg 10,Room 5B47,10 Ctr Dr, Bethesda, MD 20892 USA. EM bv10w@nih.gov NR 83 TC 38 Z9 41 U1 0 U2 6 PU KLUWER ACADEMIC/PLENUM PUBL PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD APR PY 2004 VL 9 IS 2 BP 195 EP 205 DI 10.1023/B:JOMG.0000037162.84758.b5 PG 11 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA 858NY UT WOS:000224200200008 PM 15300013 ER PT J AU Lie, RK AF Lie, RK TI Research ethics and evidence based medicine SO JOURNAL OF MEDICAL ETHICS LA English DT Article ID NICE; START AB In this paper, the author argues that the requirement to conduct randomised clinical trials to inform policy in cases where one wants to identify a cheaper alternative to known effective but expensive interventions raises an important ethical issue. This situation will eventually arise whenever there are resource constraints, and a policy decision has been made not to fund an intervention on cost effectiveness grounds. It has been thought that this is an issue only in extremely resource poor settings. This paper gives an example from the United Kingdom illustrating that this is also a problem faced by richer countries. C1 NIH, Dept Clin Bioeth, Bethesda, MD 20852 USA. RP Lie, RK (reprint author), NIH, Dept Clin Bioeth, Bldg 10,Room 1C118, Bethesda, MD 20852 USA. EM reidar.lie@fil.uib.no NR 8 TC 5 Z9 6 U1 0 U2 0 PU B M J PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 0306-6800 J9 J MED ETHICS JI J. Med. Ethics PD APR 1 PY 2004 VL 30 IS 2 BP 122 EP 125 DI 10.1136/jme.2003.007203 PG 4 WC Ethics; Medical Ethics; Social Issues; Social Sciences, Biomedical SC Social Sciences - Other Topics; Medical Ethics; Social Issues; Biomedical Social Sciences GA 812AV UT WOS:000220813300003 PM 15082802 ER PT J AU Lie, RK Emanuel, E Grady, C Wendler, D AF Lie, RK Emanuel, E Grady, C Wendler, D TI The standard of care debate: the Declaration of Helsinki versus the international consensus opinion SO JOURNAL OF MEDICAL ETHICS LA English DT Article ID PLACEBO-CONTROLLED TRIALS; ACTIVE-CONTROL TRIALS; PERINATAL TRANSMISSION; DEVELOPING-COUNTRIES; ETHICS; ISSUES AB The World Medical Association's revised Declaration of Helsinki endorses the view that all trial participants in every country are entitled to the worldwide best standard of care. In this paper the authors show that this requirement has been rejected by every national and international committee that has examined this issue. They argue that the consensus view now holds that it is ethically permissible, in some circumstances, to provide research participants less than the worldwide best care. Finally, the authors show that there is also consensus regarding the broad conditions under which this is acceptable. C1 Univ Bergen, Dept Publ Hlth & Primary Hlth Care, Bergen, Norway. NIH, Dept Clin Bioeth, Bethesda, MD 20892 USA. RP Lie, RK (reprint author), Univ Bergen, Dept Publ Hlth & Primary Hlth Care, Kalfarveien 31, Bergen, Norway. EM Reidar.Lie@fil.uib.no NR 15 TC 49 Z9 52 U1 0 U2 3 PU B M J PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 0306-6800 J9 J MED ETHICS JI J. Med. Ethics PD APR 1 PY 2004 VL 30 IS 2 BP 190 EP 193 DI 10.1136/jme.2003.006031 PG 4 WC Ethics; Medical Ethics; Social Issues; Social Sciences, Biomedical SC Social Sciences - Other Topics; Medical Ethics; Social Issues; Biomedical Social Sciences GA 812AV UT WOS:000220813300017 PM 15082816 ER PT J AU Marroni, F Aretini, P D'Andrea, E Caligo, MA Cortesi, L Viel, A Ricevuto, E Montagna, M Cipollini, G Ferrari, S Santarosa, M Bisegna, R Bailey-Wilson, JE Bevilacqua, G Parmigiani, G Presciuttini, S AF Marroni, F Aretini, P D'Andrea, E Caligo, MA Cortesi, L Viel, A Ricevuto, E Montagna, M Cipollini, G Ferrari, S Santarosa, M Bisegna, R Bailey-Wilson, JE Bevilacqua, G Parmigiani, G Presciuttini, S TI Evaluation of widely used models for predicting BRCA1 and BRCA2 mutations SO JOURNAL OF MEDICAL GENETICS LA English DT Article ID BREAST-CANCER RISK; OVARIAN-CANCER; FAMILY HISTORY; SUSCEPTIBILITY GENES; GERMLINE MUTATIONS; PRETEST PREDICTION; SEQUENCE-ANALYSIS; WOMEN; PREVALENCE; FREQUENCY C1 Johns Hopkins Univ, Dept Biostat, Baltimore, MD 21205 USA. Johns Hopkins Univ, Dept Oncol, Baltimore, MD 21205 USA. NHGRI, Inherited Dis Res Branch, NIH, Baltimore, MD USA. Univ Aquila, Dept Expt Med, I-67100 Laquila, Italy. IRCCS, Oncol Referral Ctr, Aviano, PN, Italy. Univ Modena & Reggio Emilia, Sect Biol Chem, Dept Biomed Sci, Modena, Italy. Univ Modena & Reggio Emilia, Dept Hematol & Oncol, Modena, Italy. Univ Padua, IST, Sect Viral & Mol Oncol, Padua, Italy. Univ Padua, Dept Oncol & Surg Sci, Sect Oncol, Padua, Italy. Univ Pisa, Ctr Stat Genet, Pisa, Italy. Univ Pisa, Sect Pathol, Dept Oncol Transplants & New Technol Med, Pisa, Italy. RP Presciuttini, S (reprint author), Ctr Retrovirus Res, Genet Stat, SS Abetone & Brennero 2, I-56127 Pisa, Italy. EM sprex@biomed.unipi.it RI Ferrari, Sergio/A-1110-2010; montagna, marco/E-2225-2012; Santarosa, Manuela/A-7300-2013; D'Andrea, Emma/B-4374-2013; OI Ferrari, Sergio/0000-0002-4822-2521; montagna, marco/0000-0002-4929-2150; Marroni, Fabio/0000-0002-1556-5907; RICEVUTO, Enrico/0000-0001-5641-3187; Bailey-Wilson, Joan/0000-0002-9153-2920 NR 38 TC 46 Z9 46 U1 0 U2 1 PU B M J PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1468-6244 J9 J MED GENET JI J. Med. Genet. PD APR 1 PY 2004 VL 41 IS 4 BP 278 EP 285 DI 10.1136/jmg.2003.013623 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA 808SP UT WOS:000220589100008 PM 15060102 ER PT J AU Mohiddin, SA Ahmed, ZM Griffith, AJ Tripodi, D Friedman, TB Fananapazir, L Morell, RJ AF Mohiddin, SA Ahmed, ZM Griffith, AJ Tripodi, D Friedman, TB Fananapazir, L Morell, RJ TI Novel association of hypertrophic cardiomyopathy, sensorineural deafness, and a mutation in unconventional myosin VI (MYO6) SO JOURNAL OF MEDICAL GENETICS LA English DT Article ID SNELLS-WALTZER MICE; HEARING-LOSS; HAIR-CELLS; DILATED CARDIOMYOPATHY; RECESSIVE DEAFNESS; HUMAN HOMOLOG; GENE; FIBROBLASTS; MIGRATION; SPECTRUM C1 NIDCD, Sect Human Genet, Mol Genet Lab, NIH, Rockville, MD 20850 USA. NIDCD, Sect Gene Struct & Funct, Genet Mol Lab, NIH, Rockville, MD 20850 USA. NHLBI, Cardiovasc Branch, NIH, Bethesda, MD 20892 USA. NIDCD, Hearing Sect, Neurootol Branch, NIH, Rockville, MD 20850 USA. RP Morell, RJ (reprint author), NIDCD, Sect Human Genet, Mol Genet Lab, NIH, 5 Res Court, Rockville, MD 20850 USA. EM morellr@helix.nih.gov OI Morell, Robert/0000-0003-1537-7356 NR 38 TC 59 Z9 62 U1 1 U2 2 PU B M J PUBLISHING GROUP PI LONDON PA BRITISH MED ASSOC HOUSE, TAVISTOCK SQUARE, LONDON WC1H 9JR, ENGLAND SN 1468-6244 J9 J MED GENET JI J. Med. Genet. PD APR 1 PY 2004 VL 41 IS 4 BP 309 EP 314 DI 10.1136/jmg.2003.011973 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA 808SP UT WOS:000220589100015 PM 15060111 ER PT J AU Loredo, ML Mita-Alban, LC Monteon, VM Escobar, JC Yu, ZX AF Loredo, ML Mita-Alban, LC Monteon, VM Escobar, JC Yu, ZX TI Nitric oxide mediates apoptosis in acute myocarditis induced by a Mexican strain of trypanosoma cruzi SO JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY LA English DT Meeting Abstract CT 26th Annual Meeting of the North American Section of the International-Society-for-Heart-Research CY MAY 02-05, 2004 CL Cancun, MEXICO SP Int Soc Heart Res, N Amer Sect C1 Inst Nacl Cardiol, Mexico City, DF, Mexico. NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2828 EI 1095-8584 J9 J MOL CELL CARDIOL JI J. Mol. Cell. Cardiol. PD APR PY 2004 VL 36 IS 4 BP 615 EP 615 PG 1 WC Cardiac & Cardiovascular Systems; Cell Biology SC Cardiovascular System & Cardiology; Cell Biology GA 817MK UT WOS:000221181400034 ER PT J AU Modi, WS Ivanov, S Gallagher, DS AF Modi, WS Ivanov, S Gallagher, DS TI Concerted evolution and higher-order repeat structure of the 1.709 (satellite IV) family in bovids SO JOURNAL OF MOLECULAR EVOLUTION LA English DT Article DE concerted evolution; satellite DNA; artiodactyls; pulsed field gels ID SYSTEMATIC RELATIONSHIPS; DNA-SEQUENCES; RECOMBINATION; ARTIODACTYLA; MICROTUS; HETEROCHROMATIN; HETEROGENEITY; ORGANIZATION; MAMMALIA AB The 1.709 or satellite IV repeated DNA family originally isolated from the domestic cow was analyzed using Southern blotting, pulsed field gel electrophoresis, fluorescence in situ hybridization, and DNA sequencing in species belonging to the genera Bos, Bison, Bubalus, Syncerus, Boselaphus, and Tragelaphus. Hybridization indicates that the family has been amplified in Bos, Bison, Bubalus, and Syncerus but not in Boselaphus or Tragelaphus. Pericentromeric, higher-order repeat substructure exists in all species, with multimeric arrays ranging in size from 10 to 1500 kb. Sequence analysis of a 492-bp PCR product revealed comparable levels (0.2-4.5%) of intra- and interspecific divergence when species of Bos and Bison were compared, supporting the idea that species of these two genera should be recognized under the genus Bos. Alternatively, all Syncerus sequences cluster as a monophyletic group on an evolutionary tree and differ from those of Bos/Bison by about 13%. Comparing these findings with the fossil record indicates that concerted evolution has occurred since Bos/Bison and Syncerus last shared a common ancestor (5.0 MYA) but before the radiation of the genus Bos (2.5 MYA): GenBank accession numbers AY517856-AY517904. C1 NCI, SAIC Frederick, Basic Res Program, Frederick, MD 21702 USA. Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. RP Modi, WS (reprint author), NCI, SAIC Frederick, Basic Res Program, Frederick, MD 21702 USA. EM modi@ncifcrf.gov FU PHS HHS [N01 C0 124000] NR 22 TC 10 Z9 10 U1 0 U2 0 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0022-2844 J9 J MOL EVOL JI J. Mol. Evol. PD APR PY 2004 VL 58 IS 4 BP 460 EP 465 DI 10.1007/s00239-003-2-567-6 PG 6 WC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Evolutionary Biology; Genetics & Heredity GA 812NQ UT WOS:000220846600009 PM 15114424 ER PT J AU Carey, MP Carey, KB Maisto, SA Schroder, KEE Vanable, PA Gordon, CM AF Carey, MP Carey, KB Maisto, SA Schroder, KEE Vanable, PA Gordon, CM TI HIV risk behavior among psychiatric outpatients - Association with psychiatric disorder, substance use disorder, and gender SO JOURNAL OF NERVOUS AND MENTAL DISEASE LA English DT Article ID PERSISTENT MENTAL-ILLNESS; SEXUAL-BEHAVIOR; SOCIAL COMPETENCE; ALCOHOL-USE; ILL ADULTS; INFECTION; PREVALENCE; WOMEN; ADOLESCENTS; PREVENTION AB People living with a mental illness are disproportionately vulnerable to human immunodeficiency virus. The current study sought to examine the influence of psychiatric disorder, substance use disorder, and gender on risky sexual behavior in this vulnerable population. Participants were 228 female and 202 male outpatients (66% mood disorder, 34% schizophrenia), each of whom took part in a Structured Clinical Interview for the DSM-IV and a comprehensive assessment of sexual risk behavior. Univariate and multivariate analyses tested a priori hypotheses. The results indicated that risk behavior was more frequent among patients diagnosed with a mood disorder (compared with those diagnosed with schizophrenia) or a substance use disorder (compared with those without a comorbid disorder) or both. We recommend routine human immunodeficiency virus risk screening and risk reduction programs for this vulnerable population. C1 Syracuse Univ, Ctr Hlth & Behav, Syracuse, NY 13244 USA. Utah State Univ, Dept Psychol, Logan, UT 84322 USA. NIMH, Ctr Mental Hlth Res AIDS, Bethesda, MD 20892 USA. RP Carey, MP (reprint author), Syracuse Univ, Ctr Hlth & Behav, 430 Huntington Hall, Syracuse, NY 13244 USA. FU NIMH NIH HHS [K02 MH001582, K02-MH01582, R01 MH054929, R01-MH54929] NR 44 TC 61 Z9 63 U1 1 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0022-3018 J9 J NERV MENT DIS JI J. Nerv. Ment. Dis. PD APR PY 2004 VL 192 IS 4 BP 289 EP 296 DI 10.1097/01.nmd.0000120888.54094.38 PG 8 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 810KB UT WOS:000220702100006 PM 15060403 ER PT J AU Terry, PD Kamel, F Umbach, DM Lehman, TA Hu, H Sandler, DP Taylor, JA AF Terry, PD Kamel, F Umbach, DM Lehman, TA Hu, H Sandler, DP Taylor, JA TI VEGF promoter haplotype and amyotrophic lateral sclerosis (ALS) SO JOURNAL OF NEUROGENETICS LA English DT Article DE vascular endothelial growth factor; amyotrophic lateral sclerosis; epidemiologic studies; haplotypes ID ENDOTHELIAL GROWTH-FACTOR AB Vascular endothelial growth factor (VEGF) is a cytokine essential for angiogenesis. A recent study found that haplotypes, determined by three SNPs (-2,578C/A, -1,154 G/A, and -634G/C) in the VEGF upstream promoter/leader sequence, were associated with risk of amyotrophic lateral sclerosis (ALS). We used samples and data from a case-control study to examine the relation of ALS to VEGF haplotype. Genotypes at each of the three polymorphic sites were determined using allele-specific primer extension reactions followed by MALDI-TOF. We found a 3-fold increased risk among individuals homozygous for the AAG or AGG haplotypes (95% CI=0.7-13.4), consistent with the findings of the previous study. Given the wide confidence interval, our findings should be interpreted cautiously. C1 NIEHS, Epidemiol Branch, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA. Karolinska Inst, Dept Med Epidemiol & Biostat, Stockholm, Sweden. NIEHS, Biostat Branch, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA. Harvard Univ, Sch Med, Boston, MA 02115 USA. Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. BioServe Biotechnol Ltd, Laurel, MD 20707 USA. NIEHS, Mol Carcinogenesis Lab, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA. RP Terry, PD (reprint author), NIEHS, Epidemiol Branch, NIH, Dept Hlth & Human Serv, POB 12233,MD A3-05, Res Triangle Pk, NC 27709 USA. EM terry2@niehs.nih.gov OI Kamel, Freya/0000-0001-5052-6615; taylor, jack/0000-0001-5303-6398; Sandler, Dale/0000-0002-6776-0018 NR 14 TC 29 Z9 31 U1 0 U2 3 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0167-7063 J9 J NEUROGENET JI J. Neurogenet. PD APR-JUN PY 2004 VL 18 IS 2 BP 429 EP 434 DI 10.1080/01677060490894450 PG 6 WC Genetics & Heredity; Neurosciences SC Genetics & Heredity; Neurosciences & Neurology GA 881SX UT WOS:000225890800003 PM 15763997 ER PT J AU Mendel, I Natarajan, K Ben-Nun, A Shevach, EM AF Mendel, I Natarajan, K Ben-Nun, A Shevach, EM TI A novel protective model against experimental allergic encephalomyelitis in mice expressing a transgenic TCR-specific for myelin oligodendrocyte glycoprotein SO JOURNAL OF NEUROIMMUNOLOGY LA English DT Article DE EAE; T cell receptor; apoptosis; transgenic; Th1/Th2 cells ID T-CELL-RECEPTOR; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; MAJOR HISTOCOMPATIBILITY COMPLEX; BASIC-PROTEIN; TH2 CELLS; MULTIPLE-SCLEROSIS; DEFICIENT MICE; TOLERANCE; DEMYELINATION; LYMPHOCYTES AB Myelin oligodendrocyte glycoprotein (MOG) is an important autoantigen in multiple sclerosis and in experimental autoimmune encephalomyelitis (EAE). We generated a T cell receptor (TCR) transgenic (Tg) mouse expressing a TCR derived from an encephalitogenic T cell clone specific for MOG(35-55). This mouse failed to develop EAE spontaneously and developed mild EAE at late onset when immunized with MOG(35-55.) The Tg T cells produced large amounts of IL-4 when stimulated with MOG(35-55) and underwent FAS/FAS-L-mediated activation-induced cell death when stimulated with MOG(35-55) and IL-12. The unique phenotype of these autoantigen-specific T cells may represent an important mechanism of protection against autoimmune disease. (C) 2004 Elsevier B.V. All rights reserved. C1 NIAID, Immunol Lab, Cellular Immunol Sect, NIH, Bethesda, MD 20892 USA. Weizmann Inst Sci, Dept Immunol, IL-76100 Rehovot, Israel. RP Shevach, EM (reprint author), NIAID, Immunol Lab, Cellular Immunol Sect, NIH, Bldg 10,Room 11N315,10 Ctr Dr, Bethesda, MD 20892 USA. EM eshevach@niaid.nih.gov NR 32 TC 10 Z9 11 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-5728 J9 J NEUROIMMUNOL JI J. Neuroimmunol. PD APR PY 2004 VL 149 IS 1-2 BP 10 EP 21 DI 10.1016/j.jneuroim.2003.12.007 PG 12 WC Immunology; Neurosciences SC Immunology; Neurosciences & Neurology GA 809RH UT WOS:000220653300002 PM 15020060 ER PT J AU Wu, T Kansaku, K Hallett, M AF Wu, T Kansaku, K Hallett, M TI How self-initiated memorized movements become automatic: A functional MRI study SO JOURNAL OF NEUROPHYSIOLOGY LA English DT Article ID POSITRON-EMISSION-TOMOGRAPHY; CEREBRAL-BLOOD-FLOW; SUPPLEMENTARY MOTOR AREA; EXTERNALLY TRIGGERED MOVEMENTS; SEQUENTIAL FINGER MOVEMENTS; MEDIAL FRONTAL-CORTEX; BASAL GANGLIA; NEURONAL-ACTIVITY; VISUOMOTOR SEQUENCE; PARKINSONS-DISEASE AB We used functional magnetic resonance imaging (fMRI) and dual tasks to investigate the physiology of how movements become automatic. Normal subjects were asked to practice some self-initiated, self-paced, memorized sequential finger movements with different complexity until they could perform the tasks automatically. Automaticity was evaluated by having subjects perform a secondary task simultaneously with the sequential movements. Our secondary task was a letter-counting task where subjects were asked to identify the number of times a target letter from the letter sequences was seen. Only the performances that achieved high accuracy in both single and dual tasks were considered automatic. The fMRI results before and after automaticity was achieved were compared. Our data showed that for both conditions, sequential movements activated similar brain regions. No additional activity was observed in the automatic condition. There was less activity in bilateral cerebellum, presupplementary motor area, cingulate cortex, left caudate nucleus, premotor cortex, parietal cortex, and prefrontal cortex during the automatic stage. These findings suggest that most of the motor network participates in executing automatic movements and that it becomes more efficient as movements become more automatic. Our results do not provide evidence for any area to become more activated for automatic movements. C1 NINDS, Human Motor Control Sect, Med Neurol Branch, NIH, Bethesda, MD 20892 USA. RP Hallett, M (reprint author), NINDS, Human Motor Control Sect, Med Neurol Branch, NIH, Bethesda, MD 20892 USA. EM hallettm@ninds.nih.gov NR 90 TC 127 Z9 128 U1 1 U2 11 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3077 J9 J NEUROPHYSIOL JI J. Neurophysiol. PD APR PY 2004 VL 91 IS 4 BP 1690 EP 1698 DI 10.1152/jn.01052.2003 PG 9 WC Neurosciences; Physiology SC Neurosciences & Neurology; Physiology GA 801HD UT WOS:000220086100024 PM 14645385 ER PT J AU Rao, M AF Rao, M TI Stem and precursor cells in the nervous system SO JOURNAL OF NEUROTRAUMA LA English DT Article DE cell replacement; neurospheres; stem cells; therapy ID NEURONAL PROGENITOR CELLS; EMBRYONIC SPINAL-CORD; ADULT HUMAN BRAIN; RESTRICTED PRECURSORS; SUBVENTRICULAR ZONE; NEURAL PRECURSORS; IN-VITRO; MULTIPOTENT; LINEAGE; ASTROCYTE AB The early-formed neural tube consists of proliferating, morphologically homogeneous cells, termed "neuroepithelial (NEP) stem cells" which generate neurons, astrocytes, and oligodenrocytes through a series of intermediate precursor cells. In addition to NEP cells, a second class of stem cells-the neurosphere-forming cell-can be isolated at later stages of development. NEP cells can differentiate into neural crest stem cells, which in turn generate PNS derivatives. NEP cells and neurosphere-forming stem cells and more restricted precursors express a characteristic spectrum of markers that can be used to characterize them. Each of these cell types can be isolated from embryonic stem (ES) cell cultures, and their behavior appears similar to cells isolated at later developmental ages. The relative advantages and disadvantages of these cells for cell replacement therapy are discussed. C1 NIA, Triad Technol Ctr, Baltimore, MD 21224 USA. RP Rao, M (reprint author), NIA, Triad Technol Ctr, 333 Cassell Dr, Baltimore, MD 21224 USA. EM raomah@grc.nia.nih.gov NR 66 TC 34 Z9 34 U1 1 U2 3 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 0897-7151 J9 J NEUROTRAUM JI J. Neurotrauma PD APR PY 2004 VL 21 IS 4 BP 415 EP 427 DI 10.1089/089771504323004566 PG 13 WC Critical Care Medicine; Clinical Neurology; Neurosciences SC General & Internal Medicine; Neurosciences & Neurology GA 814UF UT WOS:000220998900006 PM 15115591 ER PT J AU Shimoji, K Ravasi, L Schmidt, K Soto-Montenegro, ML Esaki, T Seidel, J Jagoda, E Sokoloff, L Green, MV Eckelman, WC AF Shimoji, K Ravasi, L Schmidt, K Soto-Montenegro, ML Esaki, T Seidel, J Jagoda, E Sokoloff, L Green, MV Eckelman, WC TI Measurement of cerebral glucose metabolic rates in the anesthetized rat by dynamic scanning with 18 F-FDG, the ATLAS small animal PET scanner, and arterial blood sampling SO JOURNAL OF NUCLEAR MEDICINE LA English DT Article DE small animal imaging; F-13-FDG; arterial blood sampling; dynamic scan; glucose utilization; brain ID POSITRON EMISSION TOMOGRAPHY; HIGH-RESOLUTION PET; ISOFLURANE; FLOW; IMAGES; MOUSE; MICE AB Rodent models and genetically altered mice have recently become available to study many human diseases. A sensitive and accurate PET scanner for small animals would be useful to evaluate treatment of these diseases in rodent models. To examine the feasibility of performing quantitative PET studies, we performed dynamic scans with arterial blood sampling in anesthetized rats with the ATLAS (Advanced Technology Laboratory Animal Scanner) small animal PET scanner developed at the National Institutes of Health and F-18-FDG and compared activities determined by PET scanning with those obtained by direct tissue sampling. Methods: Dynamic PET scans after a bolus of similar to48 MBq (1.3 mCi) F-18-FDG were performed in rats anesthetized with isoflurane. Arterial blood sampling was performed throughout the scanning period. At 60 min the rat was killed, and the brain was rapidly removed and dissected into 5 structures (thalamus [TH], cortex [CX], brain stem [BS], cerebellum [CB], and half brain). Activity in the tissue samples was compared with the mean activity of the last 5 min of calibrated PET data. Results: Plasma activity peaked at similar to0.2 min and then cleared rapidly. Brain activity initially rose rapidly; the rate of increase then progressively slowed until activity was approximately constant between 30 and 60 min. Recovery coefficients (MBq/mL in PET images)/(MBq/mL in tissue samples) were 0.99 +/- 0.04, 0.90 +/- 0.19, 1.01 +/- 0.24, 0.84 +/- 0.05, and 1.01 +/- 0.17, respectively, in TH, CX, BS, CB, and half brain (mean +/- SD, n = 6-9). Cerebral glucose utilization determined by Patlak analyses of PET data measured 30-60 min after injection of F-18-FDG was 31.7 +/- 5.2, 23.9 +/- 4.8, 29.9 +/- 5.0, 39.3 +/- 7.3, and 28.1 +/- 4.6 mumol/100 g/min (mean +/- SD, n = 9) in TH, CX, BS, CB, and whole brain, respectively. These results are consistent with a previous C-14-deoxyglucose study of the isoflurane-anesthetized rat. Conclusion: Expected values for glucose metabolic rates and recovery coefficients near unity suggest that quantitatively accurate dynamic F-18-FDG brain imaging can be performed in the rat with arterial blood sampling and the ATLAS small animal PET scanner. C1 NIH, Positron Emiss Tomog Dept, Ctr Clin, Bethesda, MD 20892 USA. Univ Milan, Ist Sci Radiol, Milan, Italy. NIMH, Cerebral Metab Lab, Bethesda, MD 20892 USA. Univ Gregorio Maranon, Gen Hosp, Madrid, Spain. NIH, Dept Nucl Med, Ctr Clin, Bethesda, MD USA. RP Eckelman, WC (reprint author), NIH, Positron Emiss Tomog Dept, Ctr Clin, Bldg 10,Room 1C495,10 Ctr Dr,MSC 1180, Bethesda, MD 20892 USA. EM Eckelman@nih.gov NR 19 TC 63 Z9 63 U1 0 U2 5 PU SOC NUCLEAR MEDICINE INC PI RESTON PA 1850 SAMUEL MORSE DR, RESTON, VA 20190-5316 USA SN 0161-5505 J9 J NUCL MED JI J. Nucl. Med. PD APR PY 2004 VL 45 IS 4 BP 665 EP 672 PG 8 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 810UR UT WOS:000220729700026 PM 15073264 ER PT J AU Hennig, B Toborek, M Bachas, LG Suk, WA AF Hennig, B Toborek, M Bachas, LG Suk, WA TI Emerging issues: Nutritional awareness in environmental toxicology SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Article ID HEALTH; GENOMICS; DIET AB Based on recent evidence, we hypothesize that nutrition can modulate the toxicity of environmental pollutants and thus modulate health and disease outcome associated with chemical insults. For example, certain dietary fats may increase the risk to environmental insult induced by polychlorinated biphenyls (PCBs), and fruits and vegetables, rich in antioxidant and anti-inflammatory nutrients or bioactive compounds, may provide protection. Nutritional awareness in environmental toxicology is critical, because of opportunities to develop dietary guidelines which specifically target exposed populations. Nutrition may provide the most sensible means to develop primary prevention strategies of diseases associated with environmental toxicology. (C) 2004 Elsevier Inc. All rights reserved. C1 Univ Kentucky, Coll Agr, Mol & Cell Nutr Lab, Lexington, KY 40546 USA. Univ Kentucky, Dept Surg, Lexington, KY 40546 USA. Univ Kentucky, Dept Chem, Lexington, KY 40546 USA. NIEHS, Ctr Risk & Integrated Sci, Res Triangle Pk, NC 27709 USA. RP Hennig, B (reprint author), Univ Kentucky, Coll Agr, Mol & Cell Nutr Lab, 200 WP Garrigus Bldg, Lexington, KY 40546 USA. EM bhennig@uky.edu RI Bachas, Leonidas/G-2479-2015; OI Bachas, Leonidas/0000-0002-3308-6264 FU NIEHS NIH HHS [P42 ES007380] NR 12 TC 9 Z9 10 U1 1 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD APR PY 2004 VL 15 IS 4 BP 194 EP 195 DI 10.1016/j.jnutbio.2004.01.002 PG 2 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA 900PL UT WOS:000227226700001 PM 15068811 ER PT J AU Mills, JL Schonberger, LB Wysowski, DK Brown, P Durako, SJ Cox, C Kong, FH Fradkin, JE AF Mills, JL Schonberger, LB Wysowski, DK Brown, P Durako, SJ Cox, C Kong, FH Fradkin, JE TI Long-term, mortality in the united states cohort of pituitary-derived growth hormone recipients SO JOURNAL OF PEDIATRICS LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; CHILDREN; THERAPY; DEFICIENCY AB Objective Patients who received pituitary-derived growth hormone (GH) are at excess risk of mortality from Creutzfeldt-Jakob disease. We investigated whether they were at increased risk of death from other conditions, particularly preventable conditions. Study design A cohort (N = 6107) from known US pituitary-derived GH recipients (treated 1963-1985) was studied. Deaths were identified by reports from physicians and parents and the National Death Index. Rates were compared with the expected rates for the US population standardized for race, age, and sex. Results There were 433 deaths versus 114 expected (relative risk [RR], 3.8; 95% confidence interval [CI], 3.4-4.2; P < .0001) from 1963 through 1996. Risk was increased in subjects with GH deficiency caused by any tumor (RR, 10.4; 95% CI, 9.1-12.0; P < .0001). Surprisingly, subjects with hypoglycemia treated within the first 6 months of life were at extremely high risk (RR, 18.3; 95% CI, 9.2-32.8; P < .0001), as were all subjects with adrenal insufficiency (RR, 7.1; 95% CI, 6.2-8.2; P < .0001). A quarter of all deaths were sudden and unexpected. Of the 26 cases of Creutzfeldt-Jakob disease, four cases have died since 2000. Conclusions The death rate in pituitary-derived GH recipients was almost four times the expected rate. Replacing pituitary derived GH with recombinant GH has eliminated only the risk of Creutzfeldt-Jakob disease. Hypoglycemia and adrenal insufficiency accounted for far more mortality than Creutzfeldt-Jakob disease. The large number of potentially preventable deaths in patients with adrenal insufficiency and hypo glycemia underscores the importance of early intervention when infection occurs in patients with adrenal insufficiency, and aggressive treatment of panhypopituitarism. C1 NINDS, NICHHD, Bethesda, MD 20892 USA. NIDDKD, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. US FDA, Rockville, MD 20857 USA. Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Atlanta, GA USA. RP Mills, JL (reprint author), NICHD, Pediat Epidemiol Sect, NIH, DHHS, 6100 Bldg Room 7B03, Bethesda, MD 20892 USA. EM jamesmills@nih.gov NR 14 TC 72 Z9 73 U1 0 U2 2 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-3476 EI 1097-6833 J9 J PEDIATR-US JI J. Pediatr. PD APR PY 2004 VL 144 IS 4 BP 430 EP 436 DI 10.1016/j.jpeds.2003.12.036 PG 7 WC Pediatrics SC Pediatrics GA 811GO UT WOS:000220760600015 PM 15069388 ER PT J AU Jutkiewicz, EM Eller, EB Folk, JE Rice, KC Traynor, JR Woods, JH AF Jutkiewicz, EM Eller, EB Folk, JE Rice, KC Traynor, JR Woods, JH TI alpha-opioid agonists: Differential efficacy and potency of SNC80, its 3-OH (SNC86) and 3-desoxy (SNC162) derivatives in Sprague-Dawley rats SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID RHESUS-MONKEYS; SQUIRREL-MONKEYS; DELTA; BW373U86; BINDING; SNC-80 AB The diarylpiperazine delta-opioid agonist SNC80 [(+)-4-[(alphaR)-alpha-[(2S, 5R)-2,5-dimethyl-4-(2-propenyl)-1-piperazinyl]-(3-methoxyphenyl) methyl]-N,N-diethylbenzamide] produces convulsions, antidepressant-like effects, and locomotor stimulation in rats. The present study compared the behavioral effects in Sprague-Dawley rats of SNC80 with its two derivatives, SNC86[(+)- 4-[alpha(R)-alpha-[(2S,5R)-2,5- dimethyl-4-(2-propenyl)-1-piperazinyl]-(3-hydroxyphenyl) methyl]- N,N-diethylbenzamide] and SNC162 [(+)- 4-[(alphaR)-alpha-[(2S,5R)-2,5-dimethyl-4-(2-propenyl)-1-piperazinyl]-(3-phenyl) methyl]- N,N-diethylbenzamide], which differ by one functional group located in the 3-position of the benzylic ring. In behavioral measures, these three compounds demonstrated a rank order of potency and efficacy; SNC86 was the most potent and efficacious followed by SNC80 and then SNC162. In vitro, these compounds stimulated guanosine 5'-O-(3-[S-35] thio) triphosphate ([S-35]GTPgammaS) binding in the caudate putamen of coronal brain slices from drug-naive rats as measured by in vitro autoradiography. In [S-35] GTPgammaS binding studies, SNC86 seemed to be a full agonist at the delta-opioid receptor; however, SNC162 demonstrated reduced stimulation compared with SNC86, consistent with partial agonist activity. Although SNC80 was not fully efficacious in [S-35] GTPgammaS autoradiography studies, it produced behavioral effects similar to those observed with SNC86, suggesting that the behavioral effects of SNC80 may be produced by its 3-hydroxy metabolite. C1 Univ Michigan, Sch Med, Dept Pharmacol, Ann Arbor, MI 48109 USA. NIDDKD, Med Chem Lab, NIH, Bethesda, MD 20892 USA. RP Woods, JH (reprint author), Univ Michigan, Sch Med, Dept Pharmacol, 1301 MSRB 3, Ann Arbor, MI 48109 USA. EM jhwoods@umich.edu FU NIDA NIH HHS [DA 00254, DA 07267]; NIGMS NIH HHS [GM 07767] NR 19 TC 41 Z9 41 U1 0 U2 0 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR 1 PY 2004 VL 309 IS 1 BP 173 EP 181 DI 10.1124/jpet.103.061242 PG 9 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 807DJ UT WOS:000220481900022 PM 14722329 ER PT J AU Jinsmaa, Y Okada, Y Tsuda, Y Shiotani, K Sasaki, Y Ambo, A Bryant, SD Lazarus, LH AF Jinsmaa, Y Okada, Y Tsuda, Y Shiotani, K Sasaki, Y Ambo, A Bryant, SD Lazarus, LH TI Novel 2 ', 6 '-dimethyl-L-tyrosine-containing pyrazinone opioid mimetic mu-agonists with potent antinociceptive activity in mice SO JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS LA English DT Article ID CENTRAL-NERVOUS-SYSTEM; DMT-TIC PHARMACOPHORE; RECEPTOR-BINDING ACTIVITY; BRAIN-BARRIER TRANSPORT; OPIATE RECEPTORS; ANALGESIA; PEPTIDES; MOUSE; ANTAGONIST; MORPHINE AB Novel bioactive opioid mimetic agonists containing 2', 6'-dimethyl-L-tyrosine (Dmt) and a pyrazinone ring interact with mu- and delta-opioid receptors. Compound 1 [3-(4'-Dmt-aminobutyl)-6-( 3'-Dmt-aminopropyl)-5-methyl-2(1H) pyrazinone] exhibited high mu-opioid receptor affinity and selectivity (K(i)mu = 0.021 nM and K(i)delta/ K(i)mu = 1,519, respectively), and agonist activity on guinea pig ileum (IC50 = 1.7 nM) with weaker delta-bioactivity on mouse vas deferens (IC50 = 25.8 nM). Other compounds (2- 4) had mu-opioid receptor affinities and selectivities 2- to 5-fold and 4- to 7-fold less than 1, respectively. Intracerebroventricular administration of 1 in mice exhibited potent naloxone reversible antinociception (65 to 71 times greater than morphine) in both tail-flick (TF) and hot-plate (HP) tests. Distinct opioid antagonists had differential effects on antinociception: naltrindole ( delta-antagonist) partially blocked antinociception in the TF, but it was ineffective in the HP test, whereas beta-funaltrexamine (irreversible antagonist, mu(1)/mu(2)-subtypes) but not naloxonazine (mu(1)-subtype) inhibited TF test antinociception, yet both blocked antinociception in the HP test. Our data indicated that 1 acted through mu- and delta-opioid receptors to produce spinal antinociception, although primarily through the mu(2)-receptor subtype; however, the mu(1)-receptor subtype dominates supraspinally. Subcutaneous and oral administration indicated that 1 crossed gastrointestinal and blood-brain barriers to produce central nervous system-mediated antinociception. Furthermore, daily s.c. dosing of mice with 1 for 1 week developed tolerance in a similar manner to that of morphine in TF and HP tests, implicating that 1 also acts through a similar mechanism analogous to morphine at mu-opioid receptors. C1 NIEHS, Med Chem Grp, Lab Computat Biol & Risk Anal, Res Triangle Pk, NC 27709 USA. Kobe Gakuin Univ, Nishi Ku, Kobe, Hyogo 65121, Japan. Tohoku Pharmaceut Univ, Aoba Ku, Sendai, Miyagi, Japan. RP Jinsmaa, Y (reprint author), NIEHS, Med Chem Grp, Lab Computat Biol & Risk Anal, POB 12233, Res Triangle Pk, NC 27709 USA. EM yunden@niehs.nih.gov NR 40 TC 27 Z9 29 U1 0 U2 0 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0022-3565 J9 J PHARMACOL EXP THER JI J. Pharmacol. Exp. Ther. PD APR 1 PY 2004 VL 309 IS 1 BP 432 EP 438 DI 10.1124/jpet.103.060061 PG 7 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 807DJ UT WOS:000220481900051 PM 14718580 ER PT J AU Kadekaro, AL Andrade, LNS Floeter-Winter, LM Rollag, MD Virador, V Vieira, W Castrucci, AMD AF Kadekaro, AL Andrade, LNS Floeter-Winter, LM Rollag, MD Virador, V Vieira, W Castrucci, AMD TI MT-1 melatonin receptor expression increases the antiproliferative effect of melatonin on S-91 murine melanoma cells SO JOURNAL OF PINEAL RESEARCH LA English DT Article DE melatonin; melatonin receptors; murine melanoma; S-91 cell line; tumor growth ID SIGNAL-TRANSDUCTION; MOLECULAR-BIOLOGY; HORMONE RELEASE; RESPONSES; CLONING; GROWTH; MODULATION; CALMODULIN; PITUITARY; PROTEINS AB Melatonin, a derivative of tryptophan that is present in all vertebrates, was first described in bovine pineal gland. It is known that melatonin is a highly conserved molecule, present also in unicellular organisms and plants. Several effects of melatonin have been described, including receptor- and non-receptor-mediated actions. Herein, we studied the effects of melatonin on in vitro and in vivo cell proliferation of Cloudman S-91 murine melanoma cells. We demonstrated that melatonin treatment significantly inhibits S-91 melanoma cell proliferation in vitro (EC50 = 10(-7) M) as well as reduces tumor growth in vivo. We also demonstrated that melatonin directly increases the activity of the antioxidant enzymes catalase and glutathione peroxidase. These effects are most likely triggered through the direct intracellular action of melatonin, since the presence of receptors could not be demonstrated in this cell line. Expression of MT-1 melatonin receptor by stable transfection, mediated a dramatic antiproliferative melatonin effect (EC50 = 10(-10) M) in S-91 cells. The expressed receptor is negatively coupled to the adenylyl cyclase/cyclic AMP signaling pathway via Gi protein. These results suggest that expression of the MT-1 melatonin receptor in melanoma cells is a potential alternative approach to specifically target cells in cancer therapeutic treatment. C1 Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo, Brazil. Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo, Brazil. Uniformed Serv Univ Hlth Sci, Dept Anat & Cell Biol, Bethesda, MD 20814 USA. NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. RP Kadekaro, AL (reprint author), Univ Cincinnati, Coll Med, Dept Dermatol, 231 Albert Sabin Way, Cincinnati, OH 45267 USA. EM kadekaal@email.uc.edu RI Floeter-Winter, Lucile Maria/K-2618-2012 OI Floeter-Winter, Lucile Maria/0000-0002-8954-8704 NR 43 TC 37 Z9 38 U1 0 U2 9 PU BLACKWELL MUNKSGAARD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0742-3098 J9 J PINEAL RES JI J. Pineal Res. PD APR PY 2004 VL 36 IS 3 BP 204 EP 211 DI 10.1111/j.1600-079X.2004.00119.x PG 8 WC Endocrinology & Metabolism; Neurosciences; Physiology SC Endocrinology & Metabolism; Neurosciences & Neurology; Physiology GA 801AD UT WOS:000220067900009 PM 15009512 ER PT J AU McCrae, RR Costa, PT Martin, TA Oryol, VE Rukavishnikov, AA Senin, IG Hrebickova, M Urbanek, T AF McCrae, RR Costa, PT Martin, TA Oryol, VE Rukavishnikov, AA Senin, IG Hrebickova, M Urbanek, T TI Consensual validation of personality traits across cultures SO JOURNAL OF RESEARCH IN PERSONALITY LA English DT Article; Proceedings Paper CT 3rd Annual Conference of the Association-for-Research-in-Personality CY FEB, 2003 CL LOS ANGELES, CA SP Assoc Res Personal DE cross-cultural; personality traits; self/other agreement ID SELF-OTHER AGREEMENT; NEO-PI-R; 5-FACTOR MODEL; RATINGS; PSYCHOLOGY; BIG-5; DETERMINANTS; INSTRUMENTS; PERSPECTIVE; SIMILARITY AB Cross-observer agreement on personality trait ratings has been interpreted as particularly powerful evidence of the veridicality of personality traits, but cross-cultural studies of consensual validity are relatively rare. In this article we review the available literature on cross-observer agreement on traits of the Five-Factor Model, and provide new data from Russia and the Czech Republic. Russian and Czech versions of the Revised NEO Personality Inventory showed adequate internal consistency and replicated the American factor structure and gender differences. Cross-observer correlations showed moderate to high agreement, especially for Extraversion. Despite cultural differences in individualism/collectivism that affect many psychological processes, these data suggest that personality traits exist and function in much the same way in these cultures. (C) 2003 Elsevier Science (USA). All rights reserved. C1 NIA, Lab Personal & Cognit, NIH, Gerontol Res Ctr, Baltimore, MD 21224 USA. Susquehanna Univ, Dept Psychol, Selinsgrove, PA USA. Yaroslavl State Univ, Dept Psychol, Yaroslavl, Russia. Acad Sci Czech Republ, Inst Psychol, Brno, Czech Republic. RP McCrae, RR (reprint author), NIA, Lab Personal & Cognit, NIH, Gerontol Res Ctr, Box 03,5600 Nathan Shock Dr, Baltimore, MD 21224 USA. EM jeffm@lpc.grc.nia.nih.gov RI Urbanek, Tomas/G-9427-2014; Hrebickova, Martina/H-4410-2014; OI Urbanek, Tomas/0000-0002-8807-4869; Hrebickova, Martina/0000-0003-4356-567X; Costa, Paul/0000-0003-4375-1712 NR 67 TC 74 Z9 75 U1 2 U2 33 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0092-6566 J9 J RES PERS JI J. Res. Pers. PD APR PY 2004 VL 38 IS 2 BP 179 EP 201 DI 10.1016/S0092-6566(03)00056-4 PG 23 WC Psychology, Social SC Psychology GA 778MT UT WOS:000189245900006 ER PT J AU Iyer, LM Leipe, DD Koonin, EV Aravind, L AF Iyer, LM Leipe, DD Koonin, EV Aravind, L TI Evolutionary history and higher order classification of AAA plus ATPases SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA ID ATP-DEPENDENT PROTEASE; MULTIPLE SEQUENCE ALIGNMENT; DNA-REPLICATION INITIATION; CRYSTAL-STRUCTURE; BINDING PROTEINS; ESCHERICHIA-COLI; P-LOOP; HEXAMERIZATION DOMAIN; ANGSTROM RESOLUTION; MAGNESIUM CHELATASE AB The AAA+ ATPases are enzymes containing a P-loop NTPase domain, and function as molecular chaperones, ATPase subunits of proteases, helicases or nucleic-acid-stimulated ATPases. All available sequences and structures of AAA+ protein domains were compared with the aim of identifying the definitive sequence and structure features of these domains and inferring the principal events in their evolution. An evolutionary classification of the AAA+ class was developed using standard phylogenetic methods, analysis of shared sequence and structural signatures, and similarity-based clustering. This analysis resulted in the identification of 26 major families within the AAA+ ATPase class. We also describe the position of the AAA+ ATPases with respect to the RecA/F1, helicase superfamilies I/II, PilT, and ABC classes of P-loop NTPases. The AAA+ class appears to have undergone an early radiation into the clamp-loader, DnaA/Orc/Cdc6, classic AAA, and "pre-sensor 1 beta-hairpin" (PS1BH) clades. Within the PS1BH clade, chelatases, MoxR, YifB, McrB, Dynein-midasin, NtrC, and MCMs form a monophyletic assembly defined by a distinct insert in helix-2 of the conserved ATPase core, and additional helical segment between the core ATPase domain and the C-terminal alpha-helical bundle. At least 6 distinct AAA+ proteins, which represent the different major clades, are traceable to the last universal common ancestor (LUCA) of extant cellular life. Additionally, superfamily III helicases, which belong to the PS1BH assemblage, were probably present at this stage in virus-like "selfish" replicons. The next major radiation, at the base of the two prokaryotic kingdoms, bacteria an. archaea, gave rise to several distinct chaperones, ATPase subunits of proteases, DNA helicases, and transcription factors. The third major radiation, at the outset of eukaryotic evolution, contributed to the origin of several eukaryote-specific adaptations related to nuclear and cytoskeletal functions. The new relationships and previously undetected domains reported here might provide new leads for investigating the biology of AAA+ ATPases. Published by Elsevier Inc. C1 NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. RP Aravind, L (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. EM aravind@ncbi.nlm.nih.gov NR 110 TC 429 Z9 440 U1 5 U2 41 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 11 EP 31 DI 10.1016/j.jsb.2003.10.010 PG 21 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600003 PM 15037234 ER PT J AU Wang, Q Song, CC Li, CCH AF Wang, Q Song, CC Li, CCH TI Molecular perspectives on p97-VCP: progress in understanding its structure and diverse biological functions SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA ID VALOSIN-CONTAINING PROTEIN; NF-KAPPA-B; TRANSITIONAL ENDOPLASMIC-RETICULUM; UBIQUITIN-MEDIATED PROTEOLYSIS; BOUND TRANSCRIPTION FACTOR; MITOTIC GOLGI FRAGMENTS; AAA-ATPASE P97; MEMBRANE-FUSION; TYROSINE PHOSPHORYLATION; CELL-CYCLE AB The 97-kDa valosin-containing protein (p97 or VCP) is a type-II AAA ((A) over bar TPases (a) over bar ssociated with a variety of (a) over bar ctivities) ATPases, which are characterized by possessing two conserved ATPase domains. VCP forms a stable homo-hexameric structure, and this two-tier ring-shaped complex acts as a molecular chaperone that mediates many seemingly unrelated cellular activities. The involvement of VCP in the ubiquitin-proteasome degradation pathway and the identification of VCP cofactors provided us important clues to the understanding of how this molecular chaperone works. In this review, we summarize the reported biological functions of VCP and explore the molecular mechanisms underlying the diverse cellular functions. We discuss the structural and biochemical studies, and elucidate how this sophisticated enzymatic machine converts chemical energy into the mechanical forces required for the chaperone activity. Published by Elsevier Inc. C1 NCI, Basic Res Program, SAIC Frederick, Frederick, MD 21702 USA. Natl Canc Inst, Basic Res Lab, Frederick, MD 21702 USA. RP Li, CCH (reprint author), NCI, Basic Res Program, SAIC Frederick, Frederick, MD 21702 USA. EM licc@ncifcrf.gov FU NCI NIH HHS [N01-CO-12400] NR 96 TC 209 Z9 213 U1 0 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 44 EP 57 DI 10.1016/j.jsb.2003.11.014 PG 14 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600005 PM 15037236 ER PT J AU Botos, I Melnikov, EE Cherry, S Khalatova, AG Rasulova, FS Tropea, JE Maurizi, MR Rotanova, TV Gustchina, A Wlodawer, A AF Botos, I Melnikov, EE Cherry, S Khalatova, AG Rasulova, FS Tropea, JE Maurizi, MR Rotanova, TV Gustchina, A Wlodawer, A TI Crystal structure of the AAA(+) alpha domain of E-coli Lon protease at 1.9 angstrom resolution SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA DE ATP-dependent proteases; domain structure; structure comparisons; structure conservation; substrate recognition ID ATP-DEPENDENT PROTEASE; DNA-POLYMERASE-III; SITE-DIRECTED MUTAGENESIS; THERMUS-THERMOPHILUS HB8; STRUCTURE REFINEMENT; ANGSTROM RESOLUTION; NUCLEOTIDE-BINDING; SUBSTRATE-BINDING; MIGRATION MOTOR; QUALITY-CONTROL AB The crystal structure of the small, mostly helical alpha domain of the AAA(+) module of the Escherichia coli ATP-dependent protease Lon has been solved by single isomorphous replacement combined with anomalous scattering and refined at 1.9 Angstrom resolution to a crystallographic R factor of 17.9%. This domain, comprising residues 491-584, was obtained by chymotrypsin digestion of the recombinant full-length protease. The alpha domain of Lon contains four alpha helices and two parallel strands and resembles similar domains found in a variety of ATPases and helicases, including the oligomeric proteases Hs1VU and ClpAP. The highly conserved "sensor-2" Arg residue is located at the beginning of the third helix. Detailed comparison with the structures of 11 similar domains established the putative location of the nucleotide-binding site in this first fragment of Lon for which a crystal structure has become available. (C) 2003 Elsevier Inc. All rights reserved. C1 NCI, Ctr Macromol Crystallog, Frederick, MD 21702 USA. Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia. NCI, Cell Biol Lab, Bethesda, MD 20892 USA. RP Wlodawer, A (reprint author), NCI, Ctr Macromol Crystallog, MCL Bldg 536,Rm 5, Frederick, MD 21702 USA. EM wlodawer@ncifcrf.gov NR 67 TC 55 Z9 60 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 113 EP 122 DI 10.1016/j.jsb.2003.09.003 PG 10 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600012 PM 15037242 ER PT J AU Xia, D Esser, L Singh, SK Guo, FS Maurizi, MR AF Xia, D Esser, L Singh, SK Guo, FS Maurizi, MR TI Crystallographic investigation of peptide binding sites in the N-domain of the ClpA chaperone SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA DE ClpA; AAA(+); N-domain; ClpS; crystal; binding mechanism ID CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; TERMINAL DOMAIN; HSP100 CHAPERONE; QUALITY-CONTROL; AAA-ATPASE; PROTEIN; COMPLEX; PROTEASES; TRANSLOCATION AB Escherichia coli ClpA, an Hsp100/Clp chaperone and an integral component of the ATP-dependent ClpAP protease, participates in the dissolution and degradation of regulatory proteins and protein aggregates. ClpA consists of three functional domains: an N-terminal domain and two ATPase domains, D1 and D2. The N-domain is attached to D1 by a mobile linker and is made up of two tightly bound, identically folded alpha-helical bundles related by a pseudo 2-fold symmetry. Between the halves of the pseudo-dimer is a large flexible acidic loop that becomes better ordered upon binding of the small adaptor protein, ClpS. We have identified a number of structural features in the N-domain, including a Zn++ binding motif, several interfaces for binding to ClpS, and a prominent hydrophobic surface area that binds peptides in different configurations. These structural motifs may contribute to binding of protein or peptide substrates with weak affinity and broad specificity. Kinetic studies comparing wild-type ClpA to a mutant ClpA with its N-domain deleted show that the N-domains contribute to the binding of a non-specific protein substrate but not of a folded substrate with the specific SsrA recognition tag. A functional model is proposed in which the N-domains in ClpA function as tentacles to weakly hold on to proteins thereby enhancing local substrate concentration. Published by Elsevier Inc. C1 NCI, Canc Res Ctr, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. RP Xia, D (reprint author), 37 Convent Dr,Bldg 37,Room 1B22, Bethesda, MD 20892 USA. EM dixia@helix.nih.gov; mmaurizi@helix.nih.gov NR 43 TC 36 Z9 37 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 166 EP 179 DI 10.1016/j.jsb.2003.11.025 PG 14 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600018 PM 15037248 ER PT J AU Ishikawa, T Maurizi, MR Steven, AC AF Ishikawa, T Maurizi, MR Steven, AC TI The N-terminal substrate-binding domain of ClpA unfoldase is highly mobile and extends axially from the distal surface of ClpAP protease SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA DE cryo-electron microscopy; difference imaging; segmental mobility; translocation pathway; entropic brush ID ATP-DEPENDENT PROTEOLYSIS; P97 AAA ATPASE; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; DENSITY MAPS; CHAPERONE; COMPLEX; TRANSLOCATION; MICROSCOPY; RESOLUTION AB ClpAP is a barrel-like complex consisting of hexameric rings of the ClpA ATPase stacked on the double heptameric ring of ClpP peptidase. ClpA has two AAA+ domains (D1 and D2) and a 153-residue N-domain. Substrate proteins bind to the distal surface of ClpA and are unfolded and translocated axially into ClpP. To gain insight into the functional architecture of ClpA in the ATPgammaS state, we have determined its structure at 12 A resolution by cryo-electron microscopy. The resulting model has two tiers, corresponding to rings of D1 and D2 domains: oddly, there is no sign of the N-domains in the density map. However, they were detected as faint diffuse density distal to the D1 tier in a difference image between wild-type ClpAP and a mutant lacking the N-domain. This region is also accentuated in a variance map of ClpAP and in a difference imaging experiment with ClpAP complexed with ClpS, a 12 kDa protein that binds to the N-domain. These observations demonstrate that the N-domains are highly mobile. From molecular modeling, we identify their median position and estimate that they undergo fluctuations of at least 30 Angstrom. We discuss the implications of these observations for the role of N-domains in substrate binding: either they effect an initial transient binding, relaying substrate to a second site on the D1 tier where unfolding ensues, or they may serve as an entropic brush to clear the latter site of non-specifically bound ligands or substrates bound in non-productive complexes. Published by Elsevier Inc. C1 NIAMSD, Struct Biol Res Lab, Bethesda, MD 20892 USA. NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. RP Steven, AC (reprint author), NIAMSD, Struct Biol Res Lab, Bethesda, MD 20892 USA. EM Alasdair_Steven@nih.gov RI Ishikawa, Takashi/E-5023-2017 OI Ishikawa, Takashi/0000-0002-1976-7477 NR 51 TC 35 Z9 35 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 180 EP 188 DI 10.1016/j.jsb.2003.11.018 PG 9 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600019 PM 15037249 ER PT J AU Ortega, J Lee, HS Maurizi, MR Steven, AC AF Ortega, J Lee, HS Maurizi, MR Steven, AC TI ClpA and ClpX ATPases bind simultaneously to opposite ends of ClpP peptidase to form active hybrid complexes SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA DE ATP-dependent proteolysis; electron microscopy; chaperone; ClpAP protease; hybrid ClpXAP protease; ClpXP protease ID ESCHERICHIA-COLI; DEPENDENT PROTEASES; CHAPERONE ACTIVITY; CRYSTAL-STRUCTURE; QUALITY-CONTROL; PROTEIN; DEGRADATION; RECOGNITION; TRANSLOCATION; PROTEOLYSIS AB The Escherichia coli ATP-dependent ClpAP and ClpXP proteases are composed of a single proteolytic component, ClpP, complexed with either of the two related chaperones, ClpA or ClpX. ClpXP and ClpAP complexes interact with different specific substrates and catalyze ATP-dependent protein unfolding and degradation. In vitro in the presence of ATP or ATPgammaS, ClpA and ClpX form homomeric rings of six subunits, which bind to one or both ends of the double heptameric rings of ClpP. We have observed that, when equimolar amounts of ClpA and ClpX hexamers are added to ClpP in vitro in the presence of ATP or ATPgammaS, hybrid complexes in which ClpX and ClpA are bound to opposite ends of the same ClpP are readily formed. The distribution of homomeric and heteromeric complexes was consistent with random binding of ClpA and ClpX to the ends of ClpP. Direct demonstration of the functionality of the heteromeric complexes was obtained by electron microscopy, which allowed us to visualize substrate translocation into proteolytically inactive ClpP chambers. Starting with hybrid complexes to which protein substrates specific to ClpX or ClpA were bound, translocation of both types of substrates was shown to occur without significant redistribution of ClpA or ClpX. The stoichiometric ratios of the ClpA, ClpX, and ClpP oligomeric complexes in vivo are consistent with the predominance of heteromeric complexes in growing cells. Thus, ClpXAP is a bifunctional protease whose two ends can independently target different classes of substrates. Published by Elsevier Inc. C1 NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. NIAMSD, Struct Biol Lab, Bethesda, MD 20892 USA. RP Maurizi, MR (reprint author), NCI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. EM mmaurizi@helix.nih.gov NR 41 TC 39 Z9 39 U1 0 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 217 EP 226 DI 10.1016/j.jsb.2003.11.023 PG 10 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600022 PM 15037252 ER PT J AU Indig, FE Partridge, JJ von Kobbe, C Aladjem, MI Latterich, M Bohr, VA AF Indig, FE Partridge, JJ von Kobbe, C Aladjem, MI Latterich, M Bohr, VA TI Werner syndrome protein directly binds to the AAA ATPase p97/VCP in an ATP-dependent fashion SO JOURNAL OF STRUCTURAL BIOLOGY LA English DT Article; Proceedings Paper CT 5th International Conference on AAA(plus) Proteins CY JUN, 2003 CL Warrenton, VA DE cdc48p; p97; VCP; Werner syndrome; AAA domain; GFP ID ORGANELLE MEMBRANE-FUSION; DNA HELICASE ACTIVITY; CRYSTAL-STRUCTURE; TERMINAL DOMAIN; SYNDROME GENE; CELL-CYCLE; COMPLEX; P97; NSF; ER AB We have previously shown that the Werner syndrome helicase, WRNp, a member of the RecQ helicase family, forms a tight molecular complex with the p97/Valosin containing protein (VCP), a member of the AAA (ATPases associated with diverse cellular activities) family of proteins. This interaction is disrupted by chemical agents that confer DNA damage, suggesting that VCP plays an important role in the signal-dependent release of WRNp from its nucleolar sequestration site. Here, we characterized the structural requirements for interactions between WRNp and VCP and for the nuclear localization of VCP. We discovered that VCP directly binds to the RQC (RecQ conserved) domain of WRNp, which is a highly conserved motif common to the RecQ helicase family. This interaction is ATP-dependent, suggesting that VCP plays a mechanistic role in releasing WRNp from the nucleolus. Immunohistochemical analysis of various VCP domains and mutated proteins expressed in vitro demonstrated that VCP may contain several hierarchical cellular localization motifs within its domain structure. Published by Elsevier Inc. C1 NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. NCI, Cell Biol Lab, CCR, NIH, Bethesda, MD 20892 USA. NCI, Mol Pharmacol Lab, CCR, NIH, Bethesda, MD 20892 USA. McGill Univ, Dept Anat & Cell Biol, Montreal, PQ H3A 2B2, Canada. RP Indig, FE (reprint author), NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. EM indigfr@grc.nia.nih.gov RI Aladjem, Mirit/G-2169-2010 OI Aladjem, Mirit/0000-0002-1875-3110 NR 46 TC 29 Z9 30 U1 1 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1047-8477 J9 J STRUCT BIOL JI J. Struct. Biol. PD APR-MAY PY 2004 VL 146 IS 1-2 BP 251 EP 259 DI 10.1016/j.jsb.2003.11.009 PG 9 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808DA UT WOS:000220548600026 PM 15037256 ER PT J AU Ehrlich, A Booher, S Becerra, Y Borris, DL Figg, WD Turner, ML Blauvelt, A AF Ehrlich, A Booher, S Becerra, Y Borris, DL Figg, WD Turner, ML Blauvelt, A TI Micellar paclitaxel improves severe psoriasis in a prospective phase II pilot study SO JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY LA English DT Article ID COLLAGEN-INDUCED ARTHRITIS; RHEUMATOID-ARTHRITIS; ANTITUMOR-ACTIVITY; DRUG PACLITAXEL; ELUTING STENT; TAXOL; INHIBITION; TAXANE; CANDIDATE; AGENTS AB Background: Taxanes (eg, paclitaxel) are chemotherapeutic agents that have antiproliferative, antiangiogenic, and antiinflammatory properties. Objective: We sought to explore the safety and efficacy of paclitaxel in individuals with severe psoriasis. Methods: An open-label, prospective, phase II pilot study was conducted at the National Institutes of Health Clinical Center, a federal government medical research facility, in Bethesda, Maryland. Twelve patients with severe psoriasis, as defined by a baseline Psoriasis Area and Severity Index (PASI) score of greater than or equal to 20), were studied. Initially, patients received 6 intravenous infusions of micellar paclitaxel, 75 mg/m(2), at 4-week intervals (stage I). Later patients received 9 intravenous infusions of micellar paclitaxel at 2-week intervals (37.5 mg/m(2) for 3 doses followed by 50 mg/m(2) for six additional closes) (stage II). The primary end point was the percent change in the PASI from week 0 to week 24 in stage I and from week 0 to week 20 in stage II. Results: In stage 1, all 5 patients improved (mean = 59.7% decrease in PASI, median = 59.6%, range: 40.3%-79.2%). Four of the 7 patients completed stage II and all of these patients improved (mean = 45.9% decrease in PASI, median = 45.0%, range: 14.6%-79.1%). Micellar paclitaxel was well tolerated by most patients. Conclusions: Micellar paclitaxel demonstrates therapeutic activity in patients with severe psoriasis. C1 NCI, Dermatol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. NCI, Med Branch, Ctr Canc Res, Bethesda, MD 20892 USA. NCI, Ctr Canc Res, Bethesda, MD 20892 USA. RP Blauvelt, A (reprint author), Bldg 10,Room 12N238,10 Ctr Dr MSC 1908, Bethesda, MD 20892 USA. EM blauvelt@mail.nih.gov RI Figg Sr, William/M-2411-2016; OI Ehrlich, Alison/0000-0002-4062-5289 NR 28 TC 29 Z9 31 U1 0 U2 0 PU MOSBY, INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 USA SN 0190-9622 J9 J AM ACAD DERMATOL JI J. Am. Acad. Dermatol. PD APR PY 2004 VL 50 IS 4 BP 533 EP 540 DI 10.1016/j.jaad.2003.09.018 PG 8 WC Dermatology SC Dermatology GA 808CU UT WOS:000220548000002 PM 15034502 ER PT J AU Tock, CL Holland, SM Puck, JM Turners, ML AF Tock, CL Holland, SM Puck, JM Turners, ML TI A man with distinctive facial features and recurrent pyoderma, pneumonia, and skeletal fractures SO JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY LA English DT Article ID HYPER-IGE SYNDROME; CYTOKINE C1 NCI, Dermatol Branch, CCR, Bethesda, MD 20892 USA. NIAID, Bethesda, MD 20892 USA. NHGRI, Bethesda, MD 20892 USA. RP Tock, CL (reprint author), NCI, Dermatol Branch, CCR, Bldg 10,Room 12N238,10 Ctr Dr MSC 1908, Bethesda, MD 20892 USA. EM tockc@mail.nih.gov NR 7 TC 1 Z9 1 U1 0 U2 0 PU MOSBY, INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 USA SN 0190-9622 J9 J AM ACAD DERMATOL JI J. Am. Acad. Dermatol. PD APR PY 2004 VL 50 IS 4 BP 627 EP 629 DI 10.1016/j.jaad.2003.09.003 PG 3 WC Dermatology SC Dermatology GA 808CU UT WOS:000220548000015 PM 15034515 ER PT J AU Weinstein, SJ Vogt, TM Gerrior, SA AF Weinstein, SJ Vogt, TM Gerrior, SA TI Healthy Eating Index scores are associated with blood nutrient concentrations in the third national health and nutrition examination survey SO JOURNAL OF THE AMERICAN DIETETIC ASSOCIATION LA English DT Article ID GUIDELINES-FOR-AMERICANS; MAJOR CHRONIC DISEASE; DIET QUALITY INDEX; PLASMA CAROTENOIDS; VEGETABLE INTAKE; SUPPLEMENT USE; ENERGY-INTAKE; OLDER ADULTS; VITAMIN-C; FRUIT AB Objectives The Healthy Eating Index (HEI) is a summary measure of dietary quality, based on a 100-point scale. Our objectives were to assess the HEI as a measure of dietary status through its correlation with nutritional biomarkers and to identify those biomarkers most associated with diet quality and healthful food intake patterns. Design National Health and Nutrition Examination Survey (NHANES) III, 1988-94. Subjects Adults ( greater than or equal to17 years) with calculated HEI scores and blood nutrient data (n=16,467). Statistical Analyses Performed Weighted crude and partial Pearson correlation coefficients (r) between HEI scores and blood nutrients were calculated. Geometric mean blood nutrient concentrations were calculated for five HEI score categories (ranging from less than or equal to50 to >80). Results HEI score was positively correlated with serum (r=0.25) and red blood cell (r=0.27) folate, serum vitamins C (r=0.30) and E (r=0.21), and all serum carotenoids except lycopene (r=0.17 to 0.27). These blood nutrient concentrations were 21% to 175% higher for participants in the highest HEI score group (>80) compared with those in the lowest group (less than or equal to50). Mean HEI scores were significantly (P<.0001) greater among the 42% of participants who took dietary supplements. Most correlations were attenuated when adjusted for additional factors. Conclusions HEI score is correlated with a wide range of blood nutrients; the strongest relationships are with biomarkers of fruit and vegetable intake. These results are an important step in the validation of the HEI, emphasizing its potential as a tool for nutrition and health studies. C1 USDA, Ctr Nutr Policy & Promot, Alexandria, VA 22302 USA. NCI, Nutr Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,Dept Hlth & Human Serv, Bethesda, MD 20892 USA. Ctr Dis Control & Prevent, Epidem Intelligence Serv, Div Viral Hepatitis, Natl Ctr Infect Dis, Atlanta, GA USA. RP Gerrior, SA (reprint author), USDA, Ctr Nutr Policy & Promot, 3101 Pk Ctr Dr, Alexandria, VA 22302 USA. EM shirley.gerrior@cnpp.usda.gov NR 55 TC 77 Z9 81 U1 0 U2 12 PU AMER DIETETIC ASSOC PI CHICAGO PA 216 W JACKSON BLVD #800, CHICAGO, IL 60606-6995 USA SN 0002-8223 J9 J AM DIET ASSOC JI J. Am. Diet. Assoc. PD APR PY 2004 VL 104 IS 4 BP 576 EP 584 DI 10.1016/j.jada.2004.01.005 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 809KE UT WOS:000220634800016 PM 15054343 ER PT J AU Brach, JS Simonsick, EM Kritchevsky, S Yaffe, K Newman, AB AF Brach, JS Simonsick, EM Kritchevsky, S Yaffe, K Newman, AB CA Hlth Aging Body Composition Study TI The association between physical function and lifestyle activity and exercise in the health, aging and body composition study SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article; Proceedings Paper CT 54th Annual Scientific Meeting of the Gerontological-Society-of-America CY NOV, 2001 CL CHICAGO, IL SP Gerontol Soc Amer DE physical activity; exercise; physical function ID CORONARY HEART-DISEASE; OLDER ADULTS; FITNESS; WOMEN; MEN AB Objectives: To determine whether older adults who exercise demonstrate higher levels of physical function than those who do not exercise but are physically active throughout the day. Design: Cross-sectional examination of baseline data from the Health, Aging and Body Composition (Health ABC) study. Setting: Health ABC field centers in Pittsburgh, Pennsylvania, and Memphis, Tennessee. Participants: Three thousand seventy-five well-functioning black and white men and women aged 70 to 79. Measurements: Physical activity and exercise were assessed using a modified leisure-time physical activity questionnaire. Participants were classified as inactive (reporting <1,000 kcal/wk of exercise activity and less than or equal to2,719 kcal/wk of total physical activity), lifestyle active (reporting <1,000 kcal/wk of exercise activity and >2,719 kcal/wk of total physical activity), or exerciser (reportinggreater than or equal to1,000 kcal/wk of exercise activity). Physical function measures included the Established Populations for the Epidemiologic Studies of the Elderly (EPESE) battery, the Health ABC battery, a 400-m walk test, and isokinetic strength testing of the knee extensors. Results: The lifestyle active and exerciser groups had similar total activity levels (men: 6,135 kcal/wk and 6,734 kcal/wk, respectively; P=.108; women: 5,695 kcal/wk and 5,854 kcal/wk, respectively; P=.335). When examining lower extremity performance in relation to physical activity, a progressive trend was evident, with the inactive individuals most likely to have impaired performance on the EPESE battery (men: 33.7%, 24.3%, and 19.1%, P<.001; women: 49.9%, 37.3%, and 28.4%, P<.001; inactive, lifestyle active, and exerciser, respectively). Progressive trends of similar magnitude were present for the Health ABC battery, time to walk 400 m, and knee extensor strength. In multivariate linear regression, those in the inactive and lifestyle active groups had poorer scores on the Health ABC performance battery than individuals in the exercise group after controlling for demographic factors and prevalent disease (men: inactive beta=-0.27, P<.001, lifestyle active beta=-0.07, P=.032; women: inactive beta=-0.23, P<.001, lifestyle active beta=-0.07, P<.059). After controlling for demographic factors and prevalent disease, the lifestyle active and exercisers had similar proportions of functionally limited older persons (scoring <10 on the EPESE battery). Conclusion: Older adults who participate in 20 to 30 minutes of moderate-intensity exercise on most days of the week have better physical function than older persons who are active throughout the day or who are inactive. Any type of physical activity is better than no activity for protection against functional limitations, but exercise confers greater benefit for physical capacity. C1 Univ Pittsburgh, Sch Med, Dept Phys Therapy, Pittsburgh, PA 15260 USA. Univ Pittsburgh, Sch Med, Dept Epidemiol, Pittsburgh, PA USA. Univ Pittsburgh, Sch Med, Div Geriatr Med, Pittsburgh, PA USA. NIA, Intramural Res Program, Bethesda, MD 20892 USA. Jonhs Hopkins Sch Med, Dept Med, Div Geriatr Med & Gerontol, Baltimore, MD USA. Univ Tennessee, Dept Prevent Med, Memphis, TN USA. Univ Calif San Francisco, Prevent Sci Grp, San Francisco, CA 94143 USA. RP Brach, JS (reprint author), Univ Pittsburgh, Sch Med, Dept Phys Therapy, 6035 Forbes Tower, Pittsburgh, PA 15260 USA. EM jbrach@pitt.edu RI Newman, Anne/C-6408-2013 OI Newman, Anne/0000-0002-0106-1150 FU NIA NIH HHS [N01-AG-2103, N01-AG-2102, N01-AG-2106] NR 21 TC 161 Z9 170 U1 1 U2 16 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 BP 502 EP 509 DI 10.1111/j.1532-5415.2004.52154.x PG 8 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 805GO UT WOS:000220355000003 PM 15066063 ER PT J AU Weyant, RJ Newman, AB Kritchevsky, SB Bretz, WA Corby, PM Ren, DX Weissfeld, L Rubin, SM Harris, T AF Weyant, RJ Newman, AB Kritchevsky, SB Bretz, WA Corby, PM Ren, DX Weissfeld, L Rubin, SM Harris, T TI Periodontal disease and weight loss in older adults SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article DE periodontal disease; weight loss; elderly ID ORAL-HEALTH-PROBLEMS; INDEX; POPULATION; NUTRITION; OBESITY; IMPACT; EXTENT; FOOD AB Objectives: To determine the association between periodontal disease and weight loss in an elderly cohort. Design: A longitudinal design was used with participants from the Health, Aging and Body Composition (Health ABC) cohort study to determine the association between periodontal disease status and weight loss of at least 5% of baseline body weight over a period of 2 years. Setting: Participants were examined in research clinics in Pittsburgh, Pennsylvania, and Memphis, Tennessee. Participants: A randomly selected subset of 1,053 individuals from the Health ABC examination, aged 65 and older, ambulatory and community-dwelling at baseline. Measurements: Periodontal disease was measured as mean pocket depth and attachment loss, extent (percentage) of pockets with at least 6 mm probing depth, extent of bleeding on probing, and tissue inflammation. Results: In logistic regression models adjusting for variables that may explain weight loss, extent of periodontal pockets with at least 6 mm probing depth showed a significant association with weight loss (odds ratio=1.53, 95% confidence interval=1.32-1.77). Conclusion: Periodontal disease may be causally related to weight loss in the elderly and thus may increase risk of morbidity and mortality. C1 Univ Pittsburgh, Dept Dent Publ Hlth, Pittsburgh, PA 15261 USA. Univ Pittsburgh, Div Geriatr Med, Pittsburgh, PA USA. Univ Pittsburgh, Dept Biostat, Pittsburgh, PA 15261 USA. Univ Pittsburgh, Grad Sch Publ Hlth, Pittsburgh, PA USA. Univ Tennessee, Ctr Hlth Sci, Dept Prevent Med, Memphis, TN 38163 USA. Univ Calif San Francisco, Prevent Sci Grp, San Francisco, CA 94143 USA. NIA, Lab Epidemiol Biometry & Demog, NIH, Bethesda, MD 20892 USA. RP Weyant, RJ (reprint author), Univ Pittsburgh, Dept Dent Publ Hlth, 3501 Terrace St,Room 346, Pittsburgh, PA 15261 USA. EM rjw1@pitt.edu RI Newman, Anne/C-6408-2013 OI Newman, Anne/0000-0002-0106-1150 FU NIA NIH HHS [N01-AG-6-210, N01-AG-6-2103, N01-AG-6-2106] NR 26 TC 21 Z9 21 U1 2 U2 2 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 BP 547 EP 553 DI 10.1111/j.1532-5415.2004.52160.x PG 7 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 805GO UT WOS:000220355000009 PM 15066069 ER PT J AU Salerno, JA Cooley, SG AF Salerno, JA Cooley, SG TI Tribute to Marsha Goodwin-Beck, RN-C, MA, MSN SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Biographical-Item C1 NIA, NIH, Bethesda, MD 20892 USA. US Dept Vet Affairs, Geriatr Res & Evaluat Geriatr & Extended Care Str, Washington, DC USA. RP Salerno, JA (reprint author), NIA, NIH, Bldg 31,Rm 5C35,MSC 2292, Bethesda, MD 20892 USA. EM salernoj@nia.nih.gov NR 1 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 BP 623 EP 624 DI 10.1111/j.1532-5415.2004.52189.x PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 805GO UT WOS:000220355000022 ER PT J AU Ferrucci, L Guralnik, JM Studenski, S Fried, LP Cutler, GB Walston, JD AF Ferrucci, L Guralnik, JM Studenski, S Fried, LP Cutler, GB Walston, JD CA Interventions Frailty Working Grp TI Designing randomized, controlled trials aimed at preventing or delaying functional decline and disability in frail, older persons: A consensus report SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Article DE frailty; disability; prevention; randomized, controlled trials ID COMPREHENSIVE GERIATRIC ASSESSMENT; LOWER-EXTREMITY FUNCTION; QUALITY-OF-LIFE; ALZHEIMERS-DISEASE; ELDERLY PERSONS; PHYSICAL PERFORMANCE; WOMENS HEALTH; SUBSEQUENT DISABILITY; CONSORT STATEMENT; CLINICAL RESEARCH AB The discovery of effective interventions to prevent or delay disability in older persons is a public health priority. Most likely to benefit from such interventions are frail individuals who are not yet disabled and those with early disability who are at high risk of progression. In spite of this frail older persons have often been excluded from research on the assumption that they would not tolerate testing or benefit from treatment. The Interventions on Frailty Working Group developed recommendations to screen, recruit, evaluate, and retain frail older persons in clinical trials. Specific recommendations are: Eligibility screening should include a multistage process, to quickly exclude those who are too well and those who are too sick. Inclusion criteria should target those most likely to benefit, be meaningful to clinicians, and reflect advancements in the frailty research area. Disability outcome measures should include self-reported, objective, and proxy measures. Strategies to improve retention and compliance and to monitor their effectiveness should be an integral part of the study design. Estimation of cost and sample size should contemplate high dropout rates and interference bycompeting outcomes. Additional research is needed to refine criteria for screening frail older persons, identify objective measures of disability that are reliable and valid in frail older persons, and improve the informed consent process for high-risk participants, recognizing that research in this subgroup is essential to improving their health outcomes. C1 NIA, Clin Res Branch, NIH, Baltimore, MD 21224 USA. NIA, Lab Epidemiol Demog & Biometry, NIH, Bethesda, MD 20892 USA. Univ Pittsburgh, Pittsburgh, PA USA. Johns Hopkins Univ, Sch Med, Baltimore, MD USA. Johns Hopkins Univ, Sch Publ Hlth, Baltimore, MD USA. Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA. RP Ferrucci, L (reprint author), NIA, Clin Res Branch, NIH, Baltimore, MD 21224 USA. EM ferruccilu@grc.nia.nih.gov NR 70 TC 466 Z9 479 U1 7 U2 40 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 BP 625 EP 634 DI 10.1111/j.1532-5415.2004.52174.x PG 10 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 805GO UT WOS:000220355000023 PM 15066083 ER PT J AU Harris, TB AF Harris, TB TI Cholesterol and health in old age: Risk factor or risk marker? SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Editorial Material ID HONOLULU-HEART-PROGRAM; SERUM-CHOLESTEROL; FOLLOW-UP; MORTALITY; CANCER; ASSOCIATION; POPULATION; DISEASE; PEOPLE; COHORT C1 NIA, Geriatr Epidemiol Sect, Lab Epidemiol Demog & Biometry, Intramural Res Program, Bethesda, MD 20892 USA. RP Harris, TB (reprint author), NIA, Geriatr Epidemiol Sect, Lab Epidemiol Demog & Biometry, Intramural Res Program, Bethesda, MD 20892 USA. NR 18 TC 3 Z9 3 U1 1 U2 1 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 BP 639 EP 640 DI 10.1111/j.1532-5415.2004.52176.x PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 805GO UT WOS:000220355000025 PM 15066086 ER PT J AU Andreas, K Newman, AB Kriska, A Brach, J Krishnaswami, S Kritchevsky, SB Schwartz, A Harris, TB Goodpaster, BH AF Andreas, K Newman, AB Kriska, A Brach, J Krishnaswami, S Kritchevsky, SB Schwartz, A Harris, TB Goodpaster, BH TI Skeletal muscle fatigue in older adults: The Health ABC study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Univ Pittsburgh, Pittsburgh, PA USA. Univ Tennessee, Memphis, TN USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. NIA, Bethesda, MD 20892 USA. RI Brach, Jennifer/A-6912-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA P154 BP S70 EP S70 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100200 ER PT J AU Cesari, M Visser, M Newman, A Satterfield, S Rubin, S Simonsick, E Harris, T Pahor, M AF Cesari, M Visser, M Newman, A Satterfield, S Rubin, S Simonsick, E Harris, T Pahor, M TI Prognostic value of usual gait speed in well-functioning elders. Results from the Health ABC study. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Wake Forest Univ, Winston Salem, NC 27109 USA. Vrije Univ Amsterdam, Amsterdam, Netherlands. Univ Pittsburgh, Pittsburgh, PA USA. Univ Tennessee, Memphis, TN USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. NIA, IRP, Baltimore, MD 21224 USA. NIA, EDBP, Bethesda, MD 20892 USA. RI Cesari, Matteo/A-4649-2008 OI Cesari, Matteo/0000-0002-0348-3664 NR 0 TC 1 Z9 1 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA A41 BP S15 EP S16 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100042 ER PT J AU Gambassi, G Cesari, M Bartali, B Corsi, A Lauretani, F Bandinelli, S Onder, G Volpato, S Pahor, M Ferrucci, L AF Gambassi, G Cesari, M Bartali, B Corsi, A Lauretani, F Bandinelli, S Onder, G Volpato, S Pahor, M Ferrucci, L TI Association between serum lipids and inflammatory markers. Results from the InCHIANTI study SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Univ Sacred Heart, I-00168 Rome, Italy. Brown Univ, Sch Med, Dept Community Hlth, Ctr Gerontol & Hlth Care Res, Providence, RI 02912 USA. Wake Forest Univ, Bowman Gray Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. INRCA Geriatr Dept, Lab Clin Epidemiol, Florence, Italy. Univ Ferrara, I-44100 Ferrara, Italy. NIA, Longitudinal Studies Sect, Clin Res Branch, Baltimore, MD 21224 USA. RI Cesari, Matteo/A-4649-2008 OI Cesari, Matteo/0000-0002-0348-3664 NR 0 TC 0 Z9 0 U1 0 U2 2 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA P419 BP S160 EP S160 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100464 ER PT J AU Gonzalgo, SR Simonsick, E Ostir, GV Markides, KS AF Gonzalgo, SR Simonsick, E Ostir, GV Markides, KS TI ADL disability and lower extremity function predict mortality in Mexican American older adults. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Johns Hopkins Univ, Sch Med, Div Gerontol & Geriatr Med, Baltimore, MD USA. NIA, Bethesda, MD 20892 USA. Univ Texas, Med Branch, Galveston, TX 77550 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA P464 BP S175 EP S175 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100508 ER PT J AU Kritchevsky, SB Nicklas, BJ Visser, M Simonsick, EM Newman, AB Harris, TB Penninx, B Satterfield, S Colbert, L Rubin, SM Pahor, M AF Kritchevsky, SB Nicklas, BJ Visser, M Simonsick, EM Newman, AB Harris, TB Penninx, B Satterfield, S Colbert, L Rubin, SM Pahor, M TI Angiotensin Converting Enzyme Insertion/Deletion genotype, exercise and physical decline: Evidence of a gene-environment interaction. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Wake Forest Univ, Bowman Gray Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. VU Univ Med Ctr, Amsterdam, Netherlands. Univ Pittsburgh, Pittsburgh, PA USA. NIA, Bethesda, MD 20892 USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. Univ Tennessee, Memphis, TN USA. Univ Wisconsin, Madison, WI USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA A29 BP S11 EP S11 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100030 ER PT J AU Lo, PC McDermott, MM Greenland, P Liu, K Guralnik, JM Criqui, MH Pearce, WH Chan, C Ridker, P Rifai, N Schneider, J Taylor, LM Martin, GJ AF Lo, PC McDermott, MM Greenland, P Liu, K Guralnik, JM Criqui, MH Pearce, WH Chan, C Ridker, P Rifai, N Schneider, J Taylor, LM Martin, GJ TI Increasing D-dimer levels predict greater functional impairment in patients with and without peripheral arterial disease (PAD). SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. NIA, Baltimore, MD 21224 USA. Univ Calif San Diego, San Diego, CA 92103 USA. Oregon Hlth & Sci Univ, Portland, OR 97201 USA. Harvard Univ, Sch Med, Boston, MA USA. Evanston NW Hosp, Evanston, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA P565 BP S211 EP S211 PG 1 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100610 ER PT J AU Newman, AB Kupelian, V Visser, M Simonsick, E Goodpaster, B Kritchevsky, SB Tylavsky, F Rubin, SM Harris, T AF Newman, AB Kupelian, V Visser, M Simonsick, E Goodpaster, B Kritchevsky, SB Tylavsky, F Rubin, SM Harris, T TI Strength, muscle mass and mortality in the Health ABC cohort. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Univ Pittsburgh, Pittsburgh, PA USA. Vrije Univ Amsterdam, Amsterdam, Netherlands. NIA, Baltimore, MD 21224 USA. Wake Forest Univ, Winston Salem, NC 27109 USA. Univ Tennessee, Memphis, TN USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. NIA, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA A28 BP S10 EP S11 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100029 ER PT J AU Zieman, SJ Gerstenblith, G Najjar, SS Schulman, SP Capprioti, A Townsend, SN Cosgriff, RE Lakatta, EG AF Zieman, SJ Gerstenblith, G Najjar, SS Schulman, SP Capprioti, A Townsend, SN Cosgriff, RE Lakatta, EG TI Arterial stiffness, but not diastolic function, predicts exercise tolerance. SO JOURNAL OF THE AMERICAN GERIATRICS SOCIETY LA English DT Meeting Abstract CT Annual Meeting of the American-Geriatrics-Society CY MAY 17-21, 2004 CL Las Vegas, NV SP Amer Geriatr Soc C1 Johns Hopkins Univ, Baltimore, MD USA. NIA, Gerontol Res Ctr, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0002-8614 J9 J AM GERIATR SOC JI J. Am. Geriatr. Soc. PD APR PY 2004 VL 52 IS 4 SU S MA A17 BP S6 EP S7 PG 2 WC Geriatrics & Gerontology; Gerontology SC Geriatrics & Gerontology GA 813HV UT WOS:000220899100018 ER PT J AU Knepper, MA Nielsen, S AF Knepper, MA Nielsen, S TI Peter Agre, 2003 Nobel Prize winner in chemistry SO JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY LA English DT Editorial Material ID INTEGRAL MEMBRANE-PROTEIN; CELL CHIP28 PROTEIN; WATER CHANNEL; AQUAPORIN CHIP; COLLECTING DUCT; RAT-KIDNEY; VASOPRESSIN; PURIFICATION; PERMEABILITY; EXPRESSION AB Peter C. Agre, an American Society of Nephrology member, is the recipient of the 2003 Nobel Prize in Chemistry for his discovery of the aquaporin water channels. The function of many cells requires that water move rapidly into and out of them. There was only. indirect evidence that proteinaceous channels provide this vital activity until Agre and colleagues purified aquaporin-1 from human erythrocytes and reported its cDNA sequence. They proved that aquaporin-1 is a specific water channel by cRNA expression studies in Xenopus oocytes and by functional reconstitution of transport activity in liposomes after the incorporation of the purified protein. These findings sparked a veritable explosion of work that affects several long-standing areas of investigation such as the biophysics of water permeation across cell membranes, the structural biology of integral membrane proteins, the physiology of fluid transport in the kidney and other organs, and the pathophysiological basis of inherited and acquired disorders of water balance. Agre's discovery of the first water channel has spurred a revolution in animal and plant physiology and in medicine. C1 NHLBI, NIH, Bethesda, MD 20892 USA. Univ Aarhus, Water & Salt Res Ctr, DK-8000 Aarhus C, Denmark. RP Knepper, MA (reprint author), NHLBI, NIH, Bldg 10,Room 6N260,10 Ctr Dr,MSC 1603, Bethesda, MD 20892 USA. EM knep@helix.nih.gov FU Intramural NIH HHS [Z01 HL001285-21, Z99 HL999999] NR 23 TC 8 Z9 9 U1 1 U2 6 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1046-6673 J9 J AM SOC NEPHROL JI J. Am. Soc. Nephrol. PD APR PY 2004 VL 15 IS 4 BP 1093 EP 1095 DI 10.1097/01.ASN.0000118814.47663.7D PG 3 WC Urology & Nephrology SC Urology & Nephrology GA 804RX UT WOS:000220316900031 PM 15034115 ER PT J AU Moore, M Ahmed, T Glazer, A AF Moore, M Ahmed, T Glazer, A TI Using an automated knowledge agent for reference and customer service SO JOURNAL OF THE MEDICAL LIBRARY ASSOCIATION LA English DT Article C1 Natl Lib Med, Reference & Customer Serv, Bethesda, MD 20894 USA. RP Moore, M (reprint author), Univ Texas, Hlth Sci Ctr, Mail Code,7703 Floyd Curl Dr, San Antonio, TX 78229 USA. EM moorem@mail.nlm.nih.gov; ahmedt@mail.nlm.nih.gov; glazera@mail.nlm.nih.gov NR 2 TC 1 Z9 1 U1 0 U2 0 PU MEDICAL LIBRARY ASSOC PI CHICAGO PA 65 EAST WACKER PLACE, STE 1900, CHICAGO, IL 60601-7298 USA SN 1536-5050 J9 J MED LIBR ASSOC JI J. Med. Libr. Assoc. PD APR PY 2004 VL 92 IS 2 BP 271 EP 273 PG 3 WC Information Science & Library Science SC Information Science & Library Science GA 811NF UT WOS:000220777900020 PM 15098059 ER PT J AU Miller, MW Miller, RK Battaglia, LF Dewey, WC Edwards, MJ Nyborg, WL Cox, C Abramowicz, JS AF Miller, MW Miller, RK Battaglia, LF Dewey, WC Edwards, MJ Nyborg, WL Cox, C Abramowicz, JS TI The Delta T thermal dose concept 1: in vivo teratogenesis SO JOURNAL OF THERMAL BIOLOGY LA English DT Article DE thermal dose; birth defects; neural tube closure; rats; maternal effects; mammalian cell lines ID NEURAL-TUBE DEFECTS; GUINEA-PIGS; INDUCED HYPERTHERMIA; CONGENITAL DEFECTS; HEAT EXPOSURE; BIRTH-DEFECTS; CELL-CYCLE; RAT; MALFORMATIONS; SENSITIVITY AB This project was an initial test of the hypothesis that there would be a AT thermal dose relationship for birth defects in rats (Rattus norvegicus) during neural tube closure (NTC). Additionally, the same thermal dose as applied during NTC in utero in vivo, was also applied to exteriorized (i.e., ex vivo) in utero pregnant uterine horns at a comparable stage of organogenesis. Since the yields of the two regimens were comparable, the hyperthermia-induced teratogenic effects appear to result from the thermal dosing of the in utero embryos and not the elevated temperature of the mother. The hypothesis was supported. (C) 2004 Elsevier Ltd. All rights reserved. C1 Univ Rochester, Sch Med & Dent, Dept Obstet & Gynecol, Rochester, NY 14642 USA. Univ Calif San Francisco, Med Ctr, Lab Radiat Oncol, San Francisco, CA 94306 USA. Univ Sydney, Dept Vet Clin Sci, Sydney, NSW 2006, Australia. Univ Vermont, Dept Phys, Burlington, VT 05405 USA. NICHHD, Dept Hlth & Human Serv, Div Epidemiol Stat & Prevent, NIH, Bethesda, MD 20892 USA. Univ Chicago, Dept Obstet & Gynecol, Chicago, IL 60637 USA. RP Miller, MW (reprint author), Univ Rochester, Sch Med & Dent, Dept Obstet & Gynecol, 400 Elmwood Ave, Rochester, NY 14642 USA. EM morton_miller@urmc.rochester.edu NR 37 TC 9 Z9 11 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0306-4565 J9 J THERM BIOL JI J. Therm. Biol. PD APR PY 2004 VL 29 IS 3 BP 141 EP 149 DI 10.1016/j.jtherbio.2004.01.003 PG 9 WC Biology; Zoology SC Life Sciences & Biomedicine - Other Topics; Zoology GA 821YI UT WOS:000221498900002 ER PT J AU Goncalves, LF Romero, R Espinoza, J Lee, W Treadwell, M Chintala, K Brandl, H Chaiworapongsa, T AF Goncalves, LF Romero, R Espinoza, J Lee, W Treadwell, M Chintala, K Brandl, H Chaiworapongsa, T TI Four-dimensional ultrasonography of the fetal heart using color Doppler spatiotemporal image correlation SO JOURNAL OF ULTRASOUND IN MEDICINE LA English DT Article ID ULTRASOUND; FLOW; DIAGNOSIS; ECHOCARDIOGRAPHY; REGURGITATION; MANAGEMENT; DEFECTS; DISEASE AB Objective. To describe clinical and research applications of 4-dimensional imaging of the fetal heart using color Doppler spatiotemporal image correlation. Methods. Forty-four volume data sets were acquired by color Doppler spatiotemporal image correlation. Seven subjects were examined: 4 fetuses without abnormalities, 1 fetus with ventriculomegaly and a hypoplastic cerebellum but normal cardiac anatomy, and 2 fetuses with cardiac anomalies detected by fetal echocardiography (1 case of a ventricular septal defect associated with trisomy 21 and 1 case of a double-inlet right ventricle with a 46,XX karyotype). The median gestational age at the time of examination was 213/7 weeks (range, 195/7-340/7 weeks). Volume data sets were reviewed off line by multiplanar display and volume-rendering methods. Representative images and online video clips illustrating the diagnostic potential of this technology are presented. Results. Color Doppler spatiotemporal image correlation allowed multiplanar visualization of ventricular septal defects, multiplanar display and volume rendering of tricuspid regurgitation, volume rendering of the outflow tracts by color and power Doppler ultrasonography (both in a normal case and in a case of a double-inlet right ventricle with a double-outlet right ventricle), and visualization of venous streams at the level of the foramen ovale. Conclusions. Color Doppler spatiotemporal image correlation has the potential to simplify visualization of the outflow tracts and improve the evaluation of the location and extent of ventricular septal defects. Other applications include 3-dimensional evaluation of regurgitation jets and venous streams at the level of the foramen ovale. C1 NICHHD, Perinatol Res Branch, NIH, Dept Hlth & Human Serv, Detroit, MI 48201 USA. NICHHD, Perinatol Res Branch, NIH, Dept Hlth & Human Serv, Detroit, MI USA. Wayne State Univ, Hutzel Hosp, Dept Obstet & Gynecol, Detroit, MI USA. William Beaumont Hosp, Div Fetal Imaging, Royal Oak, MI 48072 USA. Wayne State Univ, Childrens Hosp Michigan, Div Cardiol, Detroit, MI USA. Kretztechn, Gen Elect Med Syst, Zipf, Austria. RP Romero, R (reprint author), NICHHD, Perinatol Res Branch, NIH, Dept Hlth & Human Serv, 4707 St Antoine Blvd, Detroit, MI 48201 USA. EM warfiela@mail.nih.gov NR 19 TC 49 Z9 54 U1 0 U2 1 PU AMER INST ULTRASOUND MEDICINE PI LAUREL PA SUBSCRIPTION DEPT, 14750 SWEITZER LANE, STE 100, LAUREL, MD 20707-5906 USA SN 0278-4297 J9 J ULTRAS MED JI J. Ultrasound Med. PD APR PY 2004 VL 23 IS 4 BP 473 EP 481 PG 9 WC Acoustics; Radiology, Nuclear Medicine & Medical Imaging SC Acoustics; Radiology, Nuclear Medicine & Medical Imaging GA 809NG UT WOS:000220642800005 PM 15098864 ER PT J AU Loaiza-Perez, AI Kenney, S Boswell, J Hollingshead, M Hose, C Linehan, WM Worrell, R Rubinstein, L Sausville, A Vistica, DT AF Loaiza-Perez, AI Kenney, S Boswell, J Hollingshead, M Hose, C Linehan, WM Worrell, R Rubinstein, L Sausville, A Vistica, DT TI Sensitivity of renal cell carcinoma to aminoflavone: Role of CYP1A1 SO JOURNAL OF UROLOGY LA English DT Article DE cytochrome P-450CYP1A1; carcinoma, renal cell; kidney; apoptosis; gene expression ID LUNG-CANCER; FLAVONOIDS AB Purpose: The aminoflavone analogue (AF) exhibits antitumor activity in vitro, particularly against neoplastic cells of renal origin. We identified cellular correlates of responsiveness to AF in continuous human tumor renal cell carcinoma lines and in tumor cell isolates, termed renal carcinoma cell strains, from patients with clear cell and papillary renal neoplasms. Materials and Methods: In vitro antiproliferative activity of AF was evaluated using the sulforhodamine B protein dye assay. In vivo antitumor activity of the drug was determined in mice bearing xenografts. Covalent binding of AF/metabolite(s) was assessed following exposure of cells to AF for 16 hours. CYP1A1 and CYP1B1 mRNA and apoptosis were quantitated by reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: AF produced total growth inhibition in vitro in 3 of 6 human tumor renal cell lines at concentrations of 90 to 400 nM. In vivo treatment of mice bearing xenografts of the Caki-1 renal cell carcinoma, sensitive to AF in vitro, resulted in significant antitumor activity, including tumor-free survivors. Studies in 13 renal cell strains isolated from patients with clear cell (9) or papillary (4) renal cell carcinoma indicated that 3 of 4 papillary strains were sensitive to AF compared with 2 of 9 clear cell strains. AF sensitive renal cell lines and strains exhibited induction of CYP1A1 and CYP1B1 gene expression, increased covalent binding of AF metabolite(s) and apoptosis. Conclusions: AF has noteworthy antitumor activity against certain human tumor renal cell lines in vitro and in vivo, which correlates with drug metabolism to covalently binding metabolites after CYP1A1 and CYP1B1 gene expression. We hypothesize that it leads to apoptosis induction. AF sensitive renal cell strains are predominantly of the papillary histological type. These results are limited by the small numbers of cell lines and cell strains but they are suggestive of the need for further testing in larger collections of cell strains. C1 NCI, Dev Therapeut Program, Div Canc Treatment & Diag, NIH, Ft Detrick, MD 21702 USA. NCI, Biometr Res Branch, Div Canc Treatment & Diag, NIH, Ft Detrick, MD 21702 USA. NCI, Urol Oncol Branch, NIH, Ft Detrick, MD 21702 USA. NCI, Screening Technol Branch, Ft Detrick, MD 21702 USA. NCI, Biol Testing Branch, Ft Detrick, MD 21702 USA. NCI, Basic Res Program, SAIC Frederick Inc, Ft Detrick, MD 21702 USA. RP Vistica, DT (reprint author), NCI, Dev Therapeut Program, Div Canc Treatment & Diag, NIH, Bldg 1052,Room 121, Ft Detrick, MD 21702 USA. EM vistica@dtpax2.ncifcrf.gov FU NCI NIH HHS [N01-CM-07002] NR 15 TC 15 Z9 15 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0022-5347 J9 J UROLOGY JI J. Urol. PD APR PY 2004 VL 171 IS 4 BP 1688 EP 1697 DI 10.1097/01.ju.0000108860.03389.1b PG 10 WC Urology & Nephrology SC Urology & Nephrology GA 805IY UT WOS:000220361200093 PM 15017268 ER PT J AU Svarovskaia, ES Barr, R Zhang, XC Pais, GCG Marchand, C Pommier, Y Burke, TR Pathak, VK AF Svarovskaia, ES Barr, R Zhang, XC Pais, GCG Marchand, C Pommier, Y Burke, TR Pathak, VK TI Azido-containing diketo acid derivatives inhibit human immunodeficiency virus type 1 integrase in vivo and influence the frequency of deletions at two-long-terminal-repeat-circle junctions SO JOURNAL OF VIROLOGY LA English DT Article ID DNA-BINDING DOMAIN; HIV-1 INTEGRASE; REVERSE-TRANSCRIPTASE; PREINTEGRATION COMPLEXES; TRANSPOSABLE ELEMENTS; NUCLEOTIDE-SEQUENCE; CRYSTAL-STRUCTURE; CATALYTIC DOMAIN; STRAND TRANSFER; RIBONUCLEASE-H AB We previously found that azido-containing beta-diketo acid derivatives (DKAs) are potent inhibitors of human immunodeficiency virus type 1 (HIV-1) integrase (IN) (X. Zhang et al., Bioorg. Med. Chem. Lett., 13:12151219, 2003). To characterize the intracellular mechanisms of action of DKAs, we analyzed the antiviral activities of two potent azido-containing DKAs with either a monosubstitution or a disubstitution of azido groups, using single- and multiple-replication-cycle assays. Both azido-containing DKAs significantly inhibited HIV-1 infection in 293T, CEM-SS, and H9 cells (50% inhibitory concentration = 2 to 13 muM) and exhibited low cytotoxicity (50% cytotoxic concentration = 60 to 600 p,M). Inhibition of HIV-1 IN in vivo was demonstrated by the observation that previously described L-708,906 resistance mutations in HIV-1 IN (T661 and T661/ S153Y) also conferred resistance to the azido-group-containing DKAs. In vitro assays and in vivo analysis indicated that the DKAs did not significantly inhibit the 3' processing and selectively inhibited the strand transfer reaction. In addition, quantitative PCR indicated that two-long-terminal-repeat (2-LTR) circles were elevated in the presence of the azido-containing DKAs, confirming that HIV-1 IN was the intracellular target of viral inhibition. To gain insight into the mechanism by which the DKAs increased 2-LTR-circle formation of 3'-processed viral DNAs, we performed extensive DNA sequencing analysis of 2-LTR-circle junctions. The results indicated that the frequency of deletions at the circle junctions was elevated from 19% for the untreated controls to 32 to 41% in the presence of monosubstituted (but not disubstituted) DKAs. These results indicate that the structure of the DKAs can influence the extent of degradation of viral DNA ends by host nucleases and the frequency of deletions at the 2-LTR-circle junctions. Thus, sequencing analysis of 2-LTR-circle junctions can elucidate the intracellular mechanisms of action of HIV-1 IN inhibitors. C1 NCI, HIV Drug Resistance Program, Canc Res Ctr, Frederick, MD 21702 USA. NCI, Med Chem Lab, Canc Res Ctr, Frederick, MD 21702 USA. NCI, Canc Res Ctr, Mol Pharmacol Lab, NIH, Bethesda, MD 20892 USA. RP Pathak, VK (reprint author), NCI, HIV Drug Resistance Program, Canc Res Ctr, Bldg 535,Rm 334, Frederick, MD 21702 USA. EM VPATHAK@ncifcrf.gov RI Burke, Terrence/N-2601-2014; Marchand, Christophe/D-8559-2016 NR 72 TC 78 Z9 84 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 7 BP 3210 EP 3222 DI 10.1128/JVI.78.7.3210-3222.2004 PG 13 WC Virology SC Virology GA 804IY UT WOS:000220293600002 PM 15016842 ER PT J AU Walters, RW Agbandje-McKenna, M Bowman, VD Moninger, TO Olson, NH Seiler, M Chiorini, JA Baker, TS Zabner, J AF Walters, RW Agbandje-McKenna, M Bowman, VD Moninger, TO Olson, NH Seiler, M Chiorini, JA Baker, TS Zabner, J TI Structure of adeno-associated virus serotype 5 SO JOURNAL OF VIROLOGY LA English DT Article ID MINK DISEASE PARVOVIRUS; MEDIATED GENE-TRANSFER; SIALIC-ACID BINDING; CANINE PARVOVIRUS; AIRWAY EPITHELIA; FUNCTIONAL IMPLICATIONS; 3-DIMENSIONAL STRUCTURE; CELLULAR RECEPTOR; PROGENITOR CELLS; EMPTY CAPSIDS AB Adeno-associated virus serotype 5 (AAV5) requires sialic acid on host cells to bind and infect. Other parvoviruses, including Aleutian mink disease parvovirus (ADV), canine parvovirus (CPV), minute virus of mice, and bovine parvovirus, also bind sialic acid. Hence, structural homology may explain this functional homology. The amino acids required for CPV sialic acid binding map to a site at the icosahedral twofold axes of the capsid. In contrast to AAV5, AAV2 does not bind sialic acid, but rather binds heparan sulfate proteoglycans at its threefold axes of symmetry. To explore the structure-function relationships among parvoviruses with respect to cell receptor attachment, we determined the structure of AAV5 by cryo-electron microscopy (cryo-EM) and image reconstruction at a resolution of 16 A. Surface features common to some parvoviruses, namely depressions encircling the fivefold axes and protrusions at or surrounding the threefold axes, are preserved in the AAV5 capsid. However, even though there were some similarities, a comparison of the AAV5 structure with those of ADV and CPV failed to reveal a feature which could account for the sialic acid binding phenotype common to all three viruses. In contrast, the overall surface topologies of AAV5 and AAV2 are similar. A pseudo-atomic model generated for AAV5 based on the crystal structure of AAV2 and constrained by the AAV5 cryo-EM envelope revealed differences only in surface loop regions. Surprisingly, the surface topologies of AAV5 and AAV2 are remarkably similar to that of ADV despite only exhibiting similar to20% identity in amino acid sequences. Thus, capsid surface features are shared among parvoviruses and may not be unique to their replication phenotypes, i.e., whether they require a helper or are autonomous. Furthermore, specific surface features alone do not explain the variability in carbohydrate requirements for host cell receptor interactions among parvoviruses. C1 Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USA. Univ Iowa, Coll Med, Dept Physiol, Iowa City, IA 52242 USA. Univ Iowa, Coll Med, Dept Biophys, Iowa City, IA 52242 USA. Univ Florida, Coll Med, Ctr Struct Biol, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA. Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. Natl Inst Dent & Craniofacial Res, Gene Therapeut Branch, NIH, Bethesda, MD 20892 USA. RP Zabner, J (reprint author), Univ Iowa, Coll Med, Dept Internal Med, 500 EMRB, Iowa City, IA 52242 USA. EM joseph-zabner@uiowa.edu FU NIAID NIH HHS [P01 AI045976]; NIDDK NIH HHS [DK54759, P30 DK054759]; NIGMS NIH HHS [GM33050, R01 GM033050, R37 GM033050] NR 53 TC 69 Z9 71 U1 1 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 7 BP 3361 EP 3371 DI 10.1128/JVI.78.7.3361-3371.2004 PG 11 WC Virology SC Virology GA 804IY UT WOS:000220293600018 PM 15016858 ER PT J AU Peletskaya, EN Kogon, AA Tuske, S Arnold, E Hughes, SH AF Peletskaya, EN Kogon, AA Tuske, S Arnold, E Hughes, SH TI Nonnucleoside inhibitor binding affects the interactions of the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase with DNA SO JOURNAL OF VIROLOGY LA English DT Article ID DOUBLE-STRANDED DNA; POLYMERASE ACTIVE-SITE; ANTIBODY FAB FRAGMENT; ANGSTROM RESOLUTION; TEMPLATE-PRIMER; CONFORMATIONAL-CHANGES; CRYSTAL-STRUCTURE; DRUG-RESISTANCE; HIV-1 RT; 8-CL TIBO AB Site-directed photoaffinity cross-linking experiments were performed by using human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) mutants with unique cysteine residues at several positions (i.e., positions 65, 67, 70, and 74) in the fingers subdomain of the p66 subunit. Since neither the introduction of the unique cysteine residues into the fingers nor the modification of the SH groups of these residues with photoaffinity cross-linking reagents caused a significant decrease in the enzymatic activities of RT, we were able to use this system to measure distances between specific positions in the fingers domain of RT and double-stranded DNA. HIV-1 RT is quite flexible. There are conformational changes associated with binding of the normal substrates and nonnucleoside RT inhibitors (NNRTIs). Cross-linking was used to monitor intramolecular movements associated with binding of an NNRTI either in the presence or in the absence of an incoming deoxynucleoside triphosphate (dNTP). Binding an incoming dNTP at the polymerase active site decreased the efficiency of cross-linking but caused only modest changes in the preferred positions of cross-linking. This finding suggests that the fingers of p66 are closer to an extended template in the "open" configuration of the enzyme with the fingers away from the active site than in the closed configuration with the fingers in direct contact with the incoming dNTP. NNRTI binding caused increased cross-linking in experiments with diazirine reagents (especially with a diazirine reagent with a longer linker) and a moderate shift in the preferred sites of interaction with the template. Cross-linking occurred closer to the polymerase active site for RTs modified at positions 70 and 74. The effects of NNRTI binding were more pronounced in the absence of a bound dNTP; pretreatment of HIV-1 RT with an NNRTI reduced the effect of dNTP binding. These observations can be explained if the binding of NNRTI causes a decrease in the flexibility in the fingers subdomain of RT-NNRTI complex and a decrease in the distance from the fingers to the template extension. C1 NCI, HIV Drug Resistance Program, Frederick, MD 21702 USA. NCI, SAIC Frederick, Frederick, MD 21702 USA. Rutgers State Univ, Dept Chem & Biol Chem, Ctr Adv Biotechnol & Med, Piscataway, NJ 08854 USA. RP Hughes, SH (reprint author), NCI, HIV Drug Resistance Program, POB B,Bldg 538, Frederick, MD 21702 USA. EM hughes@ncifcrf.gov FU NIAID NIH HHS [AI27690, AI50338-02, R37 AI027690]; NIGMS NIH HHS [GM56690, R01 GM056690] NR 44 TC 26 Z9 29 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 7 BP 3387 EP 3397 DI 10.1128/JVI.78.7.3387-3397.2004 PG 11 WC Virology SC Virology GA 804IY UT WOS:000220293600021 PM 15016861 ER PT J AU Subbarao, K McAuliffe, J Vogel, L Fahle, G Fischer, S Tatti, K Packard, M Shieh, WJ Zaki, S Murphy, B AF Subbarao, K McAuliffe, J Vogel, L Fahle, G Fischer, S Tatti, K Packard, M Shieh, WJ Zaki, S Murphy, B TI Prior infection and passive transfer of neutralizing antibody prevent replication of severe acute respiratory syndrome coronavirus in the respiratory tract of mice SO JOURNAL OF VIROLOGY LA English DT Article ID SYNCYTIAL VIRUS-INFECTION; CRITICALLY-ILL PATIENTS; INFLUENZA-VIRUS; PERITONITIS VIRUS; HEMORRHAGIC-FEVER; SARS CORONAVIRUS; HONG-KONG; IMMUNITY; PATHOGENESIS; CHALLENGE AB Following intranasal administration, the severe acute respiratory syndrome (SARS) coronavirus replicated to high titers in the respiratory tracts of BALB/c mice. Peak replication was seen in the absence of disease on day 1 or 2, depending on the dose administered, and the virus was cleared within a week. Viral antigen and nucleic acid were detected in bronchiolar epithelial cells during peak viral replication. Mice developed a neutralizing antibody response and were protected from reinfection 28 days following primary infection. Passive transfer of immune serum to naive mice prevented virus replication in the lower respiratory tract following intranasal challenge. Thus, antibodies, acting alone, can prevent replication of the SARS coronavirus in the lung, a promising observation for the development of vaccines, immunotherapy, and immunoprophylaxis regimens. C1 NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. NIH, Microbiol Serv, Ctr Clin, Bethesda, MD 20892 USA. RP Subbarao, K (reprint author), NIAID, Infect Dis Lab, NIH, Bldg 50,Room 6132,50 South Dr,MSC 8007, Bethesda, MD 20892 USA. EM Ksubbarao@niaid.nih.gov RI Tatti, Kathleen/H-5912-2012 OI Tatti, Kathleen/0000-0001-9414-7887 NR 35 TC 219 Z9 238 U1 0 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 7 BP 3572 EP 3577 DI 10.1128/JVI.78.7.3572-3577.2004 PG 6 WC Virology SC Virology GA 804IY UT WOS:000220293600040 PM 15016880 ER PT J AU Cai, ZH Liang, TJ Luo, GX AF Cai, ZH Liang, TJ Luo, GX TI Effects of mutations of the initiation nucleotides on hepatitis C virus RNA replication in the cell SO JOURNAL OF VIROLOGY LA English DT Article ID DE-NOVO INITIATION; PROTEIN 5B POLYMERASE; IN-VITRO; VIRAL-RNA; TRANSCRIPTION INITIATION; STRUCTURAL-ANALYSIS; NONCODING REGION; NS5B PROTEIN; STRAND RNA; GENOME AB Replication of nearly all RNA viruses depends on a virus-encoded RNA-dependent RNA polymerase (RdRp). Our earlier work found that purified recombinant hepatitis C virus (HCV) RdRp (NS5B) was able to initiate RNA synthesis de novo by using purine (A and G) but not pyrimidine (C and U) nucleotides (G. Luo et al., J. Virol. 74:851-863, 2000). For most human RNA viruses, the initiation nucleotides of both positive- and negative-strand RNAs were found to be either an adenylate (A) or guanylate (G). To determine the nucleotide used for initiation and control of HCV RNA replication, a genetic mutagenesis analysis of the nucleotides at the very 5' and 3' ends of HCV RNAs was performed by using a cell-based HCV replicon replication system. Either a G or an A at the 5' end of HCV genomic RNA was able to efficiently induce cell colony formation, whereas a nucleotide C at the 5' end dramatically reduced the efficiency of cell colony formation. Likewise, the 3'-end nucleotide U-to-C mutation did not significantly affect the efficiency of cell colony formation. In contrast, a U-to-G mutation at the 3' end caused a remarkable decrease in cell colony formation, and a U-to-A mutation resulted in a complete abolition of cell colony formation. Sequence analysis of the HCV replicon RNAs recovered from G418-resistant Huh7 cells revealed several interesting findings. First, the 5'-end nucleotide G of the replicon RNA was changed to an A upon multiple rounds of replication. Second, the nucleotide A at the 5' end was stably maintained among all replicon RNAs isolated from Huh7 cells transfected with an RNA with a 5'-end A. Third, initiation of HCV RNA replication with a CTP resulted in a >10-fold reduction in the levels of HCV RNAs, suggesting that initiation of RNA replication with CTP was very inefficient. Fourth, the 3'-end nucleotide U-to-C and -G mutations were all reverted back to a wild-type nucleotide U. In addition, extra U and UU residues were identified at the 3' ends of revertants recovered from Huh7 cells transfected with an RNA with a nucleotide G at the 3' end. We also determined the 5'-end nucleotide of positive-strand RNA of some clinical HCV isolates. Either G or A was identified at the 5' end of HCV RNA genome depending on the specific HCV isolate. Collectively, these findings demonstrate that replication of positive-strand HCV RNA was preferentially initiated with purine nucleotides (ATP and GTP), whereas the negative-strand HCV RNA replication is invariably initiated with an ATP. C1 Univ Kentucky, Coll Med, Dept Microbiol Immunol & Mol Genet, Lexington, KY 40536 USA. NIDDKD, Liver Dis Sect, NIH, Bethesda, MD 20892 USA. RP Luo, GX (reprint author), Univ Kentucky, Coll Med, Dept Microbiol Immunol & Mol Genet, 800 Rose St,MN475,UKMC, Lexington, KY 40536 USA. EM gluo0@uky.edu FU NCI NIH HHS [R01 CA093712, R01CA93712]; NIAID NIH HHS [R01AI51592, R01 AI051592] NR 62 TC 21 Z9 22 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 7 BP 3633 EP 3643 DI 10.1128/JVI.78.7.3633-3642.2004 PG 11 WC Virology SC Virology GA 804IY UT WOS:000220293600044 PM 15016884 ER PT J AU Troyer, JL Pecon-Slattery, J Roelke, ME Black, L Packer, C O'Brien, SJ AF Troyer, JL Pecon-Slattery, J Roelke, ME Black, L Packer, C O'Brien, SJ TI Patterns of feline immunodeficiency virus multiple infection and genome divergence in a free-ranging population of African lions SO JOURNAL OF VIROLOGY LA English DT Article ID BLOOD MONONUCLEAR-CELLS; TYPE-1 SUBTYPE-B; GENETIC DIVERSITY; DOMESTIC CAT; PHYLOGENETIC ASPECTS; SEQUENCE-ANALYSIS; VIRAL-INFECTIONS; A/G RECOMBINANT; DUAL INFECTION; HIV TYPE-1 AB Feline immunodeficiency virus (FIV) is a lentivirus that causes AIDS-like immunodeficiency disease in domestic cats. Free-ranging lions, Panthera leo, carry a chronic species-specific strain of FIV, FIV-Ple, which so far has not been convincingly connected with immune pathology or mortality. FIV-Ple, harboring the three distinct strains A, B, and C defined by pol gene sequence divergences, is endemic in the large outbred population of lions in the Serengeti ecosystem in Tanzania. Here we describe the pattern of variation in the three FIV genes gag, pol-RT, and pol-RNase among lions within 13 prides to assess the occurrence of FIV infection and coinfection. Genome diversity within and among FIV-Ple strains is shown to be large, with strain divergence for each gene approaching genetic distances observed for FIV between different species of cats. Multiple infections with two or three strains were found in 43% of the FIV-positive individuals based on pol-RT sequence analysis, which may suggest that antiviral immunity or interference evoked by one strain is not consistently protective against infection by a second. This comprehensive study of FIV-Ple in a free-ranging population of lions reveals a dynamic transmission of virus in a social species that has historically adapted to render the virus benign. C1 NCI, Lab Genom Divers, Ft Detrick, MD 21702 USA. SAIC, IRSP Program, Ft Detrick, MD 21702 USA. Univ Minnesota, Dept Ecol & Behav Biol, St Paul, MN 55108 USA. RP O'Brien, SJ (reprint author), NCI, Lab Genom Divers, Ft Detrick, MD 21702 USA. EM obrien@ncifcrf.gov RI Troyer, Jennifer/B-8415-2012 FU NCI NIH HHS [N01-CO-12400, N01CO12400] NR 57 TC 39 Z9 39 U1 1 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 7 BP 3777 EP 3791 DI 10.1128/JVI.78.7.3777-3791.2004 PG 15 WC Virology SC Virology GA 804IY UT WOS:000220293600057 PM 15016897 ER PT J AU Ramsburg, E Rose, NF Marx, PA Mefford, M Nixon, DF Moretto, WJ Montefiori, D Earl, P Moss, B Rose, JK AF Ramsburg, E Rose, NF Marx, PA Mefford, M Nixon, DF Moretto, WJ Montefiori, D Earl, P Moss, B Rose, JK TI Highly effective control of an AIDS virus challenge in macaques by using vesicular stornatitis virus and modified vaccinia virus Ankara vaccine vectors in a single-boost protocol SO JOURNAL OF VIROLOGY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL RESPONSES; RHESUS-MONKEYS; STOMATITIS-VIRUS; PRIMARY ISOLATE; NEUTRALIZING ANTIBODIES; TYPE-1; REPLICATION; PROTEINS; IMMUNIZATION AB Previous studies have shown that vaccination and boosting of rhesus macaques with attenuated vesicular stomatitis virus (VSV) vectors encoding Env and Gag proteins of simian immunodeficiency virus-human immunodeficiency virus (SHIV) hybrid viruses protect rhesus macaques from AIDS after challenge with the highly pathogenic SHIV 89.6P (23). In the present study, we compared the effectiveness of a single prime-boost protocol consisting of VSV vectors expressing SHIV Env, Gag, and Poll proteins to that of a protocol consisting of a VSV vector prime followed with a single boost with modified vaccinia virus Ankara (MVA) expressing the same SHIV proteins. After challenge with SHIV 89.6P, MVA-boosted animals controlled peak challenge viral loads to less than 2 X 10(6) copies/ml (a level significantly lower than that seen with VSV-boosted animals and lower than those reported for other vaccine studies employing the same challenge). NVA-boosted animals have shown excellent preservation of CD4(+) T cells, while two of four VSV-boosted animals have shown significant loss of CD4(+) T cells. The improved protection in MVA-boosted animals correlates with trends toward stronger prechallenge CD8(+)-T-cell responses to SHIV antigens and stronger postchallenge SHIV-neutralizing antibody production. C1 Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA. Tulane Univ, Hlth Sci Ctr, New Orleans, LA 70118 USA. Gladstone Inst Virol & Immunol, San Francisco, CA USA. Duke Univ, Med Ctr, Durham, NC 27706 USA. NIAID, Bethesda, MD 20892 USA. RP Rose, JK (reprint author), Yale Univ, Sch Med, Dept Pathol, 310 Cedar St, New Haven, CT 06510 USA. EM john.rose@yale.edu FU NIAID NIH HHS [R01 AI045510, AI-40357, AI-45510, AI-85383, R01 AI040357, R37 AI040357, R56 AI045510] NR 29 TC 78 Z9 80 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 8 BP 3930 EP 3940 DI 10.1128/JVI.78.8.3930-3940.2004 PG 11 WC Virology SC Virology GA 809MU UT WOS:000220641600015 PM 15047809 ER PT J AU Yang, ZY Chakrabarti, BK Xu, L Welcher, B Kong, WP Leung, K Panet, A Mascola, JR Nabel, GJ AF Yang, ZY Chakrabarti, BK Xu, L Welcher, B Kong, WP Leung, K Panet, A Mascola, JR Nabel, GJ TI Selective modification of variable loops alters tropism and enhances immunogenicity of human immunodeficiency virus type 1 envelope SO JOURNAL OF VIROLOGY LA English DT Article ID HUMAN MONOCLONAL-ANTIBODIES; V3 LOOP; HIV-1; NEUTRALIZATION; GP120; EPITOPE; V2; GLYCOPROTEIN; IMMUNIZATION; INFECTION AB Although the B clade of human immunodeficiency virus type 1 (HIV-1) envelopes (Env) includes five highly variable regions, each of these domains contains a subset of sequences that remain conserved. The V3 loop has been much studied for its ability to elicit neutralizing antibodies, which are often restricted to a limited number of closely related strains, likely because a large number of antigenic structures are generated from the diverse amino acid sequences in this region. Despite these strain-specific determinants, subregions of V3 are highly conserved, and the effects of different portions of the V3 loop on Env tropism and immunogenicity have not been well delineated. For this report, selective deletions in V3 were introduced by shortening of the stem of the V3 loop. These mutations were explored in combination with deletions of selected V regions. Progressive shortening of the stem of V3 abolished the immunogenicity as well as the functional activity of HIV Env; however, two small deletions on both arms of the V3 stem altered the tropism of the dualtropic 89.6P viral strain so that it infected only CXCR4(+) cells. When this smaller deletion was combined with removal of the V1 and V2 loops and used as an immunogen in guinea pigs, the antisera were able to neutralize multiple independent clade B isolates with a higher potency. These findings suggest that highly conserved subregions within V3 may be relevant targets for eliciting neutralizing antibody responses, affecting HIV tropism, and increasing the immunogenicity of AIDS vaccines. C1 NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. RP Nabel, GJ (reprint author), NIAID, Vaccine Res Ctr, NIH, Bldg 40,Room 4502,MSC 3005,40 Convent Dr, Bethesda, MD 20892 USA. EM gnabel@mail.nih.gov NR 25 TC 62 Z9 68 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 8 BP 4029 EP 4036 DI 10.1128/JVI.78.8.4029-4036.2004 PG 8 WC Virology SC Virology GA 809MU UT WOS:000220641600025 PM 15047819 ER PT J AU Chackerian, B Briglio, L Albert, PS Lowy, DR Schiller, JT AF Chackerian, B Briglio, L Albert, PS Lowy, DR Schiller, JT TI Induction of autoantibodies to CCR5 in macaques and subsequent effects upon challenge with an R5-tropic simian/human immunodeficiency virus SO JOURNAL OF VIROLOGY LA English DT Article ID REACTIVE LYMPHOCYTES-B; AMINO-TERMINAL DOMAIN; HIV-1 INFECTION; DISEASE PROGRESSION; T-CELL; CHEMOKINE RECEPTORS; GENETIC RESTRICTION; MACROPHAGE TROPISM; CORECEPTOR USAGE; RHESUS MACAQUES AB Antibodies against CCR5, the major coreceptor for human immunodeficiency virus type 1 (HIV-1), may have antiviral potential as viral fusion inhibitors. In this study, we generated a virus-like particle (VLP)-based vaccine that effectively breaks B-cell tolerance and elicits autoantibodies against CCR5 in pig-tailed macaques. Initial studies in mice identified a polypeptide comprising the N-terminal domain of pig-tailed macaque CCR5 fused to streptavidin that, when conjugated at high density to bovine papillomavirus major capsid protein L1 VLPs, induced high-titer immunoglobulin G (IgG) that bound to a macaque CCR5-expressing cell line in vitro. In macaques, CCR5 peptide-conjugated VLP preparations induced high-avidity anti-CCR5 IgG autoantibody responses, and all five immunized macaques generated IgG that could block infection of CCR5-tropic simian/human immunodeficiency virus SHIVSF162P3 in vitro. Although the anti-CCR5 IgG titers declined with time, autoantibody levels were boosted upon revaccination. Vaccinated macaques remained healthy for a period of over 3 years after the initial immunization, and no decline in the number of CCR5-expressing T cells was detected. To test the prophylactic efficacy of CCR5 autoantibodies, immunized macaques were challenged with SHIVSSF162P3. Although the plasma-associated virus in half of six control macaques declined to undetectable levels, viral loads were lower, declined more rapidly, and eventually became undetectable in all five macaques in which CCR5 autoantibodies had been elicited. In addition, in the four vaccinated macaques with higher autoantibody titers, viral loads and time to control of viremia were significantly decreased relative to controls, indicating the possibility that CCR5 autoantibodies contributed to the control of viral replication. C1 NCI, Cellular Oncol Lab, NIH, Bethesda, MD 20892 USA. NCI, Anim Sci Branch, NIH, Bethesda, MD 20892 USA. NCI, Biometr Res Branch, NIH, Bethesda, MD 20892 USA. NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Chackerian, B (reprint author), NCI, Cellular Oncol Lab, NIH, 37 Convent Dr,Room 4106, Bethesda, MD 20892 USA. EM brycec@nih.gov NR 58 TC 36 Z9 39 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 8 BP 4037 EP 4047 DI 10.1128/JVI.78.8.4037-4047.2004 PG 11 WC Virology SC Virology GA 809MU UT WOS:000220641600026 PM 15047820 ER PT J AU Cahir-McFarland, ED Carter, K Rosenwald, A Giltnane, JM Henrickson, SE Staudt, LM Kieff, E AF Cahir-McFarland, ED Carter, K Rosenwald, A Giltnane, JM Henrickson, SE Staudt, LM Kieff, E TI Role of NF-kappa B in cell survival and transcription of latent membrane protein 1 - Expressing or Epstein-Barr virus latency III-infected cells SO JOURNAL OF VIROLOGY LA English DT Article ID RBP-J-KAPPA; LYMPHOCYTE GROWTH TRANSFORMATION; ACTIVATED PROTEIN-KINASE; SIGNAL-BINDING-PROTEIN; HUMAN T-LYMPHOCYTES; GENE-EXPRESSION; MEMBRANE PROTEIN-1; NUCLEAR ANTIGEN-2; IN-VIVO; MOLECULE EXPRESSION AB Epstein-Barr virus (EBV) latency III infection converts B lymphocytes into lymphoblastoid cell lines (LCLS) by expressing EBV nuclear and membrane proteins, EBNAs, and latent membrane proteins (LMPs), which regulate transcription through Notch and tumor necrosis factor receptor pathways. The role of NF-kappaB in LMP1 and overall EBV latency III transcriptional effects was investigated by treating LCLs with BAY11-7082 (BAY11). BAY11 rapidly and irreversibly inhibited NF-kappaB, decreased mitochondriall membrane potential, induced apoptosis, and altered LCL gene expression. BAY11 effects were similar to those of an NF-kappaB inhibitor, DeltaN-IkappaBalpha, in effecting decreased JNK1 expression and in microarray analyses. More than 80% of array elements that decreased with DeltaN-IkappaBalpha expression decreased with BAY11 treatment. Newly identified NF-kappaB-induced, LMP1-induced, and EBV-induced genes included pleckstrin, Jun-B, c-FLIP, CIP4, and IkappaBepsilon. Of 776 significantly changed array elements, 134 were fourfold upregulated in EBV latency III, and 74 were fourfold upregulated with LMP1 expression alone, whereas only 28 were more than fourfold downregulated by EBV latency III. EBV latency III-regulated gene products mediate cell migration (EB12, CCR7, RGSI, RANTES, MIPIalpha, MIP1beta, CXCR5, and RGS13), antigen presentation (major histocompatibillity complex proteins and JAW1), mitogen-activated protein kinase pathway (DUSP5 and p62Dok), and interferon (IFN) signaling (IFN-gammaRalpha, IRF-4, and STAT1). Comparison of EBV latency III LCL gene expression to immunoglobulin M (IgM)-stimulated B cells, germinal-center B cells, and germinal-center-derived lymphomas clustered LCLs with IgM-stimulated B cells separately from germinal-center cells or germinal-center lymphoma cells. Expression of IRF-2, AIM1, ASK1, SNF2L2, and components of IFN signaling pathways further distinguished EBV latency III-infected B cells from IgM-stimulated or germinal-center B cells. C1 Brigham & Womens Hosp, Channing Lab, Boston, MA 02130 USA. Brigham & Womens Hosp, Div Infect Dis, Boston, MA 02130 USA. Harvard Univ, Sch Med, Dept Med, Boston, MA 02115 USA. Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA. NCI, Metab Branch, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. RP Cahir-McFarland, ED (reprint author), Brigham & Womens Hosp, Channing Lab, Boston, MA 02130 USA. EM ecahir@rics.bwh.harvard.edu RI Giltnane, Jennifer/D-2584-2013 FU NCI NIH HHS [CA85180, CA47006, CA87661, P01 CA087661, R01 CA047006, R01 CA085180, R35 CA047006]; NIGMS NIH HHS [T32 GM007753] NR 99 TC 148 Z9 154 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 8 BP 4108 EP 4119 DI 10.1128/JVI.78.8.4108-4119.2004 PG 12 WC Virology SC Virology GA 809MU UT WOS:000220641600033 PM 15047827 ER PT J AU Spann, KM Tran, KC Chi, B Rabin, RL Collins, PL AF Spann, KM Tran, KC Chi, B Rabin, RL Collins, PL TI Suppression of the induction of alpha, beta, and gamma interferons by the NS1 and NS2 proteins of human respiratory syncytial virus in human epithelial cells and macrophages SO JOURNAL OF VIROLOGY LA English DT Article ID MESSENGER-RNA; VACCINE; IFN; LIVE; CHIMPANZEES; EXPRESSION; RESPONSES; MUTANTS; GENES; RSV AB Wild-type human respiratory syncytial virus (HRSV) is a poor inducer of alpha/beta interferons (IFN-alpha/beta). However, recombinant HRSV lacking the NS1 and NS2 genes (DeltaNSI/2) induced high levels of IFN-alpha and -beta in human pulmonary epithelial cells (A549) as well as in macrophages derived from primary human peripheral blood monocytes. Results with NS1 and NS2 single- and double-gene-deletion viruses indicated that the two proteins function independently as well as coordinately to achieve the full inhibitory effect, with NS1 having a greater independent role. The relative contributions of the individual NS proteins were the converse of that recently described for bovine RSV (J. F. Valarcher, J. Furze, S. Wyld, R. Cook, K. K. Conzelmann, and G. Taylor, J. Virol. 77:8426-8439, 2003). This pattern of inhibition by HRSV NS1 and NS2 also extended to the newly described antiviral cytokines IFN-lambda1, -2 and -3. C1 NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. Ctr Biol Evaluat & Res, Food & Drug Adm, Lab Immunobiochem, Bethesda, MD 20892 USA. RP Collins, PL (reprint author), NIAID, Infect Dis Lab, NIH, Bldg 50,Room 6507,50 S Dr,MSC 8007, Bethesda, MD 20892 USA. EM pcollins@niaid.nih.gov RI Spann, Kirsten/B-4524-2013 OI Spann, Kirsten/0000-0003-0567-8382 NR 28 TC 249 Z9 264 U1 2 U2 6 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 8 BP 4363 EP 4369 DI 10.1128/JVI.78.8.4363-4396.2004 PG 7 WC Virology SC Virology GA 809MU UT WOS:000220641600056 PM 15047850 ER PT J AU Roca, AL Peacon-Slattery, J O'Brien, SJ AF Roca, AL Peacon-Slattery, J O'Brien, SJ TI Genomically intact endogenous feline leukemia viruses of recent origin SO JOURNAL OF VIROLOGY LA English DT Article ID FELV-RELATED SEQUENCES; LONG TERMINAL REPEATS; DOMESTIC CAT; SUBGROUP-B; NUCLEOTIDE-SEQUENCE; MOLECULAR-CLONING; MITOCHONDRIAL-DNA; ENVELOPE GENE; ENV ELEMENTS; RETROVIRUS AB We isolated and sequenced two complete endogenous feline leukemia viruses (enFeLVs), designated enFeLV-AGTT and enFeLV-GGAG. In enFeLV-AGTT, the open reading frames are reminiscent of a functioning FeLV genome, and the 5' and 3' long terminal repeat sequences are identical. Neither endogenous provirus is genetically fixed in cats but polymorphic, with 8.9 and 15.2% prevalence for enFeLV-AGTT and enFeLV-GGAG, respectively, among a survey of domestic cats. Neither provirus was found in the genomes of related species of the Felis genus, previously shown to harbor enFeLVs. The absence of mutational divergence, polymorphic incidence in cats, and absence in related species suggest that these enFeLVs may have entered the germ line more recently than previously believed, perhaps coincident with domestication, and reopens the question of whether some enFeLVs might be replication competent. C1 SAIC Frederick, Basic Res Program, Lab Genom Divers, Frederick, MD 21702 USA. NCI, Lab Genom Divers, Ft Detrick, MD 21702 USA. RP O'Brien, SJ (reprint author), NCI, Lab Genom Divers, Ft Detrick, MD 21702 USA. EM obrien@ncifcrf.gov FU NCI NIH HHS [N01CO12400, N01-CO-12400] NR 47 TC 32 Z9 32 U1 1 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD APR PY 2004 VL 78 IS 8 BP 4370 EP 4375 DI 10.1128/JVI.78.8.4370-4375.2004 PG 6 WC Virology SC Virology GA 809MU UT WOS:000220641600057 PM 15047851 ER PT J AU Chen, S Hoffman, BB Lee, JS Kasama, Y Jim, B Kopp, JB Ziyadeh, FN AF Chen, S Hoffman, BB Lee, JS Kasama, Y Jim, B Kopp, JB Ziyadeh, FN TI Cultured tubule cells from TGF-beta 1 null mice exhibit impaired hypertrophy and fibronectin expression in high glucose SO KIDNEY INTERNATIONAL LA English DT Article DE diabetic nephropathy; TGF-beta type I receptor; TGF-beta type II receptor; knockout ID GROWTH-FACTOR-BETA; MATRIX GENE-EXPRESSION; TGF-BETA; MESANGIAL CELLS; TRANSFORMING GROWTH-FACTOR-BETA-1; DIABETES-MELLITUS; EPITHELIAL-CELLS; PROXIMAL TUBULE; MESSENGER-RNA; ELEVATED GLUCOSE AB Background. To firmly establish the role of the transforming growth factor-beta1 (TGF-beta1) isoform in the pathophysiology of diabetic tubulointerstitial hypertrophy and fibrosis, we examined how the total absence of TGF-beta1 would alter the effect of high glucose on cellular hypertrophy and matrix expression in tubuloepithelial cells cultured from TGF-beta1 null mice. Methods. Primary tubule cell cultures, obtained from kidneys of TGF-beta1 knockout mice and their wild-type littermates, were treated with exogenous TGF-beta1 or high glucose. The TGF-beta system was characterized at the ligand and receptor levels using Northern and Western blotting. Cellular hypertrophy and growth were assessed by thymidine incorporation, cell counting, leucine incorporation, and protein content. Fibronectin expression was assessed by Northern analysis and enzyme-linked immunosorbent assay (ELISA). Results. Knockout cells did not express TGF-X1 but did express TGF-beta2, TGF-beta3, and TGF-beta type I and type II receptors. Exogenous TGF-beta1 down-regulated the ligand-binding type II receptor but up-regulated type I receptor expression. Knockout cells proliferated more rapidly than wild-type cells, but restoring TGF-beta1 to knockout cells slowed their proliferation. In wildtype cells, high glucose caused cellular hypertrophy, evidenced by greater leucine incorporation and protein content along with decreased thymidine incorporation. High glucose also increased fibronectin message and protein. However, in knockout cells, high glucose failed to induce hypertrophy and was severely limited in its capacity to stimulate fibronectin. Conclusion. In tubular epithelial cells, TGF-beta1 mediates the hypertrophic and fibronectin-stimulatory effects of high glucose, confirming the role of the TGF-beta1 isoform in the pathogenesis of diabetic tubular hypertrophy and fibronectin overexpression. C1 Univ Penn, Dept Med, Renal Electrolyte & Hypertens Div, Philadelphia, PA 19104 USA. NIDDKD, Kidney Dis Sect, NIH, Bethesda, MD 20892 USA. RP Ziyadeh, FN (reprint author), 700 Clin Res Bldg,415 Curie Blvd, Philadelphia, PA 19104 USA. EM ziyadeh@mail.med.upenn.edu FU NIDDK NIH HHS [DK-07006, DK-09993, DK-44513, DK-45191, DK-54608, DK-61537] NR 50 TC 25 Z9 26 U1 0 U2 2 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD APR PY 2004 VL 65 IS 4 BP 1191 EP 1204 DI 10.1111/j.1523-1755.2004.00492.x PG 14 WC Urology & Nephrology SC Urology & Nephrology GA 802AF UT WOS:000220135700007 PM 15086458 ER PT J AU Depner, T Daugirdas, J Greene, T Allon, M Beck, G Chumlea, C Delmez, J Gotch, F Kusek, J Levin, N Macon, E Milford, E Owen, W Star, R Toto, R Eknoyan, G AF Depner, T Daugirdas, J Greene, T Allon, M Beck, G Chumlea, C Delmez, J Gotch, F Kusek, J Levin, N Macon, E Milford, E Owen, W Star, R Toto, R Eknoyan, G CA HEMO Study Grp TI Dialysis dose and the effect of gender and body size on outcome in the HEMO Study SO KIDNEY INTERNATIONAL LA English DT Article DE clinical trial; hemodialysis; gender; race; HEMO Study; survival; body size; weight; body water volume; Kt/V ID UREA REDUCTION RATIO; HEMODIALYSIS-PATIENTS; MORTALITY; SURVIVAL; INDEX AB Background. Gender and body size have been associated with survival in hemodialysis populations. In recent observational studies, overall mortality was similar in men and women and higher in small patients. The effect of dialysis dose in each of these subgroups has not been tested in a clinical trial. Methods. The HEMO Study was a controlled trial of dialysis dose and membrane flux in 1846 hemodialysis patients followed up for 6.6 years in 15 centers throughout the United States. We examined the effect of dialysis dose on mortality and on selected secondary outcomes in subgroups of patients. Results. Adjusting for age only, overall mortality was lower in patients with higher body weight (P < 0.001), higher body mass index (P < 0.001), and higher body water content determined by the Watson formula (Vw) (P < 0.001), but was not associated with gender (P = 0.27). The RR of mortality comparing the high dose with the standard dose group was related to gender (P = 0.014). Women randomized to the high dose had a lower mortality rate than women randomized to the standard dose (RR = 0.81, P = 0.02), while men randomized to the high dose had a nonsignificant trend for a higher mortality rate than men randomized to the standard dose (RR = 1.16, P = 0.16). Analysis of both genders combined showed no overall dose effect (R = 0.96, P = 0.52), as reported previously. Vw was greater than 35 L in 84% of men compared with 17% of women. However, the RR of mortality for the high versus standard dose remained lower in women than in men after adjustment for the interaction of dose with Vw or with other size parameters, including weight and body mass index. Conversely, the dose effect was not significantly related to size parameters after controlling for the relationship of the dose comparison with gender. Conclusion. The data suggest that mortality and morbidity might be reduced by increasing the dialysis dose above the current standard in women but not in men. This effect was not explained by differences between men and women in age, race, or in several indices of body size. Because multiple comparisons were considered in this analysis, the role of gender on the effect of dialysis dose is suggestive and invites further study. C1 Univ Calif Davis, Div Nephrol, Sacramento, CA 95817 USA. Univ Illinois, Sch Med, Chicago, IL USA. Cleveland Clin Fdn, Cleveland, OH 44195 USA. Univ Alabama, Dept Nephrol, Birmingham, AL USA. Wright State Univ, Sch Med, Kettering, OH USA. Washington Univ, Dept Nephrol, St Louis, MO USA. Univ Calif San Francisco, Sch Med, San Francisco, CA USA. NIDDK, NIH, Bethesda, MD USA. Beth Israel Med Ctr, Renal Res Inst, New York, NY 10003 USA. Emory Univ Hosp, Dept Nephrol, Atlanta, GA 30322 USA. RP Depner, T (reprint author), Univ Calif Davis, Div Nephrol, 4150 V St,Suite 3500, Sacramento, CA 95817 USA. EM tadepner@ucdavis.edu NR 22 TC 87 Z9 87 U1 0 U2 2 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD APR PY 2004 VL 65 IS 4 BP 1386 EP 1394 DI 10.1111/j.1523-1755.2004.00519.x PG 9 WC Urology & Nephrology SC Urology & Nephrology GA 802AF UT WOS:000220135700028 PM 15086479 ER PT J AU Kaysen, GA Dubin, JA Muller, HG Rosales, L Levin, NW Mitch, WE AF Kaysen, GA Dubin, JA Muller, HG Rosales, L Levin, NW Mitch, WE CA HEMO Study Grp TI Inflammation and reduced albumin synthesis associated with stable decline in serum albumin in hemodialysis patients SO KIDNEY INTERNATIONAL LA English DT Article DE nutrition; inflammation; CRP; alpha 1 acid glycoprotein; ceruloplasmin; albumin synthesis ID DIETARY-PROTEIN; HOMEOSTASIS; MECHANISMS; DIALYSIS; FAILURE; SEPSIS; DEATH AB Background. The concentration of albumin in serum is maintained by its rates of synthesis, catabolism, and distribution between vascular and extravascular compartments. Albumin synthesis is suppressed when there is inflammation or inadequate protein intake. This study was conducted to establish whether a decline in serum albumin of > 0.3 g/dL was accompanied by a change in albumin synthesis and if so whether these changes were associated with increased levels of acute phase proteins and/or with a decrease in equilibrated normalized protein catabolic rate (enPCR). Methods. Seventy-nine patients in the National Institutes of Health (NIH)-sponsored HEMO Study had baseline measurements of albumin synthesis (measured kinetically as the disappearance of [(125)]I human serum albumin), the serum concentrations of albumin, transferrin, C-reactive protein (CRP), a 1 acid glycoprotein (alpha1AG), ceruloplasmin, interleukin-6 (IL-6), plus monthly measurements of enPCR. The plasma levels of all proteins and enPCR were measured regularly over 2 years or until serum albumin decreased by > 0.3 g/dL on two sequential measurements. Albumin synthesis was measured a second time when serum albumin declined by > 0.3 g/dL or after 2 years. Results. Fifty-nine patients [21 with a significant decrease in serum albumin (decliners) and 38 with stable values of serum albumin] had albumin synthesis measured twice. A decline in albumin concentration and synthesis was associated with an increase in alpha1AG when data from all patients were analyzed as a group. In decliners, albumin synthesis decreased significantly but was unchanged in stable. Likewise, in decliners, IL-6, CRP, alpha1AG, and ceruloplasmin increased significantly but were unchanged in stable. enPCR was unchanged in both groups and was not associated with either changes in albumin level or synthesis in the whole group. Conclusion. A decrease in serum albumin of > 0.3 g/dL that persists for a period of 6 weeks is associated a decrease in albumin synthesis. This response is associated with evidence of activation of the acute phase response (inflammation) but not with changes in enPCR. In well-dialyzed patients, inflammation is the principal cause of a decrease in serum albumin while protein intake plays an insignificant role. C1 Univ Calif Davis, Dept Med, Div Nephrol, TB 136, Davis, CA 95616 USA. VANCHCS, Res Serv, Mather, CA USA. Renal Res Inst New York, New York, NY USA. Yale Univ, Div Biostat, New Haven, CT USA. Univ Calif Davis, Dept Stat, Davis, CA 95616 USA. Univ Texas, Dept Med, Galveston, TX 77555 USA. NIDDK, HEMO Study Grp, Bethesda, MD 20892 USA. RP Kaysen, GA (reprint author), Univ Calif Davis, Dept Med, Div Nephrol, TB 136, Davis, CA 95616 USA. EM gakaysen@ucdavis.edu FU NCRR NIH HHS [M01 RR 00039]; NIDDK NIH HHS [R01 DK 50777] NR 25 TC 89 Z9 95 U1 0 U2 2 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD APR PY 2004 VL 65 IS 4 BP 1408 EP 1415 DI 10.1111/j.1523-1755.2004.00520.x PG 8 WC Urology & Nephrology SC Urology & Nephrology GA 802AF UT WOS:000220135700031 PM 15086482 ER PT J AU Unruh, M Miskulin, D Yan, GF Hays, RD Benz, R Kusek, JW Meyer, KB AF Unruh, M Miskulin, D Yan, GF Hays, RD Benz, R Kusek, JW Meyer, KB CA HEMO Study Grp TI Racial differences in health-related quality of life among hemodialysis patients SO KIDNEY INTERNATIONAL LA English DT Article DE quality of life; race; hemodialysis ID STAGE RENAL-DISEASE; INCIDENT DIALYSIS PATIENTS; COMORBIDITY ASSESSMENT; FUNCTIONAL HEALTH; PRACTICE PATTERNS; MENTAL-HEALTH; OUTCOMES; SURVIVAL; INDEX; HEMO AB Background. Despite technical progress in therapy, hemodialysis patients continue to report health-related quality of life (HRQOL) substantially lower than that of the general population. While African Americans with end-stage renal disease (ESRD) survive longer than members of other races, few studies have compared the HRQOL of African Americans with that of non-African Americans. Methods. We examined differences in sociodemographic, clinical, and HRQOL variables by race. A multiple regression model assessed the extent to which race was associated with differences in HRQOL scores after adjustment for sociodemographic and clinical variables. Racial differences in the relationship between comorbid disease severity and HRQOL were explored. Results. In adjusted models, African Americans had higher scores in the Index of Well-Being and burden of kidney disease, but lower scores in cognitive function (all P < 0.05). For scales reflecting symptoms and effects of kidney disease, sleep quality, and the Physical Component Summary, the fall in HRQOL with increasing comorbidity was significantly greater in non-African Americans (all P < 0.05). After adjustment, there were no racial differences in scores on the Mental Component Summary, social support, dialysis staff encouragement, or patient satisfaction. Conclusion. To our knowledge, ESRD is the only chronic illness for which African Americans report significantly better psychologic well being and a lower burden of disease than non-African Americans. Further research is needed to understand whether these experiences affect health care utilization, medical decision making, and patient survival. Clarification of the reasons for race differences may suggest measures to improve HRQOL for all patients with ESRD. C1 Univ Pittsburgh, Med Ctr, Pittsburgh, PA 15261 USA. Tufts New England Med Ctr, Boston, MA USA. Cleveland Clin, Data Coordinating Ctr, Cleveland, OH 44106 USA. Univ Calif Los Angeles, Dept Hlth Serv, Los Angeles, CA USA. Lankenau Hosp, Wynnewood, PA USA. NIDDK, Div Kidney Urol & Hematol Dis, Bethesda, MD USA. RP Unruh, M (reprint author), Univ Pittsburgh, Med Ctr, A909 Scaife Hall,3550 Terrace St, Pittsburgh, PA 15261 USA. EM unruh@pitt.edu RI Hays, Ronald/D-5629-2013; OI Meyer, Klemens/0000-0001-5253-4950 FU NIA NIH HHS [AG-02-004]; NIDDK NIH HHS [U01DK 49241, T32-DK07777, U01DK 46109, U01DK 46114, U01DK 46126, U01DK 46143, U01DK 49240, U01DK 49242, U01DK 49243, U01DK 49244, U01DK 49249, U01DK 49252, U01DK 49254, U01DK 49259, U01DK 49261, U01DK 49264, U01DK 49271]; NIMHD NIH HHS [P20-MD00148-01] NR 58 TC 59 Z9 61 U1 1 U2 4 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD APR PY 2004 VL 65 IS 4 BP 1482 EP 1491 DI 10.1111/j.1523-1755.2004.00529.x PG 10 WC Urology & Nephrology SC Urology & Nephrology GA 802AF UT WOS:000220135700041 PM 15086492 ER PT J AU Kobayashi, H Kawamoto, S Brechbiel, MW Jo, SK Hu, XZ Yang, TX Diwan, BA Waldmann, TA Schnermann, J Choyke, PL Star, RA AF Kobayashi, H Kawamoto, S Brechbiel, MW Jo, SK Hu, XZ Yang, TX Diwan, BA Waldmann, TA Schnermann, J Choyke, PL Star, RA TI Micro-MRI methods to detect renal cysts in mice SO KIDNEY INTERNATIONAL LA English DT Article DE magnetic resonance imaging; kidney; chronic kidney disease; cyst; COX-2; sickle cell disease ID POLYCYSTIC KIDNEY-DISEASE; CONTRAST AGENT; MURINE MODEL; PROGRESSION; INHIBITOR; FIESTA AB Background. Mouse models of disease, especially using transgenic and knockout technologies, are powerful tools to analyze the molecular basis of disease. We recently reported that a new dynamic micro-MRI method with dendrimer-based contrast agents can visualize renal structure and function in normal living mice and mice with acute renal failure. While MRI contrast enhancement is useful for detecting functional impairment of the kidneys, this technology has limitations in assessing morphologic changes, particularly cystic disease, because contrast-enhanced micro-MRI depicts cysts as low-intensity areas that cannot be distinguished from fibrotic foci. Methods. In the current study, we evaluated if micro-MRI employing a new three-dimensional MR hydrography signal sequence [three-dimensional fast imaging employing steady-state acquisition (3D-FIESTA)] can visualize chronic cystic changes without any contrast agents. Results. We were able to positively depict multiple renal cortical cysts of similar to0.2 mm diameter in a mouse model of sickle cell disease and observe serial changes of renal cysts ( 0.2 mm diameter) in cyclooxygenase-2 (COX-2) knockout mice during a 2 1/2-month period. Some cysts decreased in size over time. Conclusions. Micro-MRI with 3D-FIESTA can depict cyst formation in the diseased kidneys of living mice without injection of contrast agents. C1 NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21205 USA. NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, NIH, Bethesda, MD 20892 USA. NIDDK, Renal Diagnost & Therapeut Unit, NIH, Bethesda, MD USA. NIDDK, Lab Renal Funct & Dev, NIH, Bethesda, MD USA. NIH, Dept Diagnost Radiol, Ctr Clin, Bethesda, MD 20892 USA. NCI, Basic Res Program, SAIC Frederick, NIH, Frederick, MD 21701 USA. RP Kobayashi, H (reprint author), NCI, Metab Branch, Ctr Canc Res, NIH, Bldg 10,Room 4N109,10 Ctr Dr, Bethesda, MD 20892 USA. EM Kobayash@mail.nih.gov FU NCI NIH HHS [N01-CO-12400] NR 18 TC 24 Z9 26 U1 0 U2 2 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0085-2538 J9 KIDNEY INT JI Kidney Int. PD APR PY 2004 VL 65 IS 4 BP 1511 EP 1516 DI 10.1111/j.1523-1755.2004.00532.x PG 6 WC Urology & Nephrology SC Urology & Nephrology GA 802AF UT WOS:000220135700044 PM 15086495 ER PT J AU Brown, P AF Brown, P TI Fall-out from a possible transfusion-related transmission of vCJD SO LANCET NEUROLOGY LA English DT Editorial Material ID CREUTZFELDT-JAKOB-DISEASE; SPONGIFORM ENCEPHALOPATHY; BLOOD-TRANSFUSION C1 NIH, Bethesda, MD 20892 USA. RP Brown, P (reprint author), NIH, Bldg 10, Bethesda, MD 20892 USA. EM brownp@ninds.nih.gov NR 6 TC 2 Z9 2 U1 0 U2 0 PU LANCET LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1474-4422 J9 LANCET NEUROL JI Lancet Neurol. PD APR PY 2004 VL 3 IS 4 BP 203 EP 203 DI 10.1016/S1474-4422(04)00702-1 PG 1 WC Clinical Neurology SC Neurosciences & Neurology GA 814QU UT WOS:000220990000011 PM 15039029 ER PT J AU Feldman, AL Minniti, C Santi, M Downing, JR Raffeld, M Jaffe, ES AF Feldman, AL Minniti, C Santi, M Downing, JR Raffeld, M Jaffe, ES TI Histiocytic sarcoma after acute lymphoblastic leukaemia: a common clonal origin SO LANCET ONCOLOGY LA English DT Article ID TUMORS C1 NCI, Pathol Lab, Hematopathol Sect, Bethesda, MD 20892 USA. George Washington Univ, Washington, DC USA. Childrens Natl Med Ctr, Dept Hematol Oncol, Washington, DC 20010 USA. Childrens Natl Med Ctr, Dept Pathol, Washington, DC 20010 USA. St Jude Childrens Res Hosp, Dept Pathol, Memphis, TN 38105 USA. RP Feldman, AL (reprint author), NCI, Pathol Lab, Hematopathol Sect, Bldg 10,Room 2A33,10 Ctr Dr, Bethesda, MD 20892 USA. EM Andrew_Feldman@nih.gov RI Feldman, Andrew/D-5028-2012 NR 7 TC 46 Z9 49 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1470-2045 J9 LANCET ONCOL JI Lancet Oncol. PD APR PY 2004 VL 5 IS 4 BP 248 EP 250 DI 10.1016/S1470-2045(04)01428-7 PG 3 WC Oncology SC Oncology GA 811CL UT WOS:000220749900021 PM 15050956 ER PT J AU Peters, DC Guttman, MA Dick, AJ Raman, VK Lederman, RJ McVeigh, ER AF Peters, DC Guttman, MA Dick, AJ Raman, VK Lederman, RJ McVeigh, ER TI Reduced field of view and undersampled PR combined for interventional imaging of a fully dynamic field of view SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE UNFOLD; reduced field of view (rFOV); projection reconstruction; interventional MRI; radial imaging; catheter tracking; temporal filtering ID PROJECTION-RECONSTRUCTION; TEMPORAL RESOLUTION; K-SPACE; UNFOLD; MRI; PLACEMENT; MODEL AB Active catheter imaging was investigated using real-time undersampled projection reconstruction (PR) combined with the temporal filtering technique of reduced field of view (rFOV). Real-time rFOV processing was interactively enabled during highly undersampled catheter imaging, resulting in improved artifact suppression with better temporal resolution than that obtained by view-sharing, Imaging with 64 to 32 projections provided a resolution of 2 x 2 x 8 mm, and four to eight true frames per second. Image artifacts were reduced when rFOV processing was applied to the undersampled images. A comparison with Cartesian rFOV showed that PR image quality is less susceptible to aliasing that results from rFOV imaging with a wholly dynamic outer FOV. Simulations and MRI experiments demonstrated that PR rFOV provides significant artifact suppression, even for a fully dynamic FOV. The near doubling of temporal resolution that is possible with PR rFOV permits accurate monitoring of highly dynamic events, such as catheter movements, and arrhythmias, such as ventricular ectopy. Published 2004 Wiley-Liss, Inc.(dagger) C1 NHLBI, Cardiac Energet Lab, NIH, DHHS, Bethesda, MD 20892 USA. RP Peters, DC (reprint author), Beth Israel Deaconess Med Ctr, RW457,330 Brookline Ave, Boston, MA 02215 USA. EM dcpeters@bidmc.harvard.edu OI lederman, robert/0000-0003-1202-6673 FU Intramural NIH HHS [Z01 HL004608-08, Z01 HL005062-05] NR 24 TC 8 Z9 8 U1 0 U2 3 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0740-3194 J9 MAGNET RESON MED JI Magn. Reson. Med. PD APR PY 2004 VL 51 IS 4 BP 761 EP 767 DI 10.1002/mrm.20037 PG 7 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 808GI UT WOS:000220557200015 PM 15065249 ER PT J AU Jones, DK AF Jones, DK TI The effect of gradient sampling schemes on measures derived from diffusion tensor MRI: A Monte Carlo study SO MAGNETIC RESONANCE IN MEDICINE LA English DT Article DE sampling schemes; electrostatic respulsion; cone of uncertainty; trace; anisotropy; rotational invariance ID HUMAN BRAIN; ANISOTROPY; TRACKING; CONNECTIVITY; SPECTROSCOPY AB There are conflicting opinions in the literature as to whether it is more beneficial to use a large number of gradient sampling orientations in diffusion tensor MRI (DT-MRI) experiments than to use a smaller number of carefully chosen orientations. In this study, Monte Carlo simulations were used to study the effect of using different gradient sampling schemes on estimates of tensor-derived quantities assuming a b-value of 1000 smm(-2). The study focused in particular on the effect that the number of unique gradient orientations has on uncertainty in estimates of tensor-orientation, and on estimates of the trace and anisotropy of the diffusion tensor. The results challenge the recently proposed notion that a set of six icosahedrally-arranged orientations is optimal for DT-MRI. It is shown that at least 20 unique sampling orientations are necessary for a robust estimation of anisotropy, whereas at least 30 unique sampling orientations are required for a robust estimation of tensor-orientation and mean diffusivity. Finally, the performance of sampling schemes that use low numbers of sampling orientations, but make efficient use of available gradient power, are compared to less efficient schemes with larger numbers of sampling orientations, and the relevant scenarios in which each type of scheme should be used are discussed. Published 2004 Wiley-Liss, Inc.(1) C1 NICHHD, Sect Tissue Biophys & Biomimet, Lab Integrat Med & Biophys, NIH, Bethesda, MD 20892 USA. RP Jones, DK (reprint author), NICHHD, Sect Tissue Biophys & Biomimet, Lab Integrat Med & Biophys, NIH, Bldg 13,Room 3W16E,13 South Dr, Bethesda, MD 20892 USA. EM jonesde@mail.nih.gov RI Jones, Derek/D-1460-2009; OI Jones, Derek/0000-0003-4409-8049 NR 30 TC 417 Z9 429 U1 2 U2 12 PU JOHN WILEY & SONS INC PI HOBOKEN PA 111 RIVER ST, HOBOKEN, NJ 07030 USA SN 0740-3194 J9 MAGNET RESON MED JI Magn. Reson. Med. PD APR PY 2004 VL 51 IS 4 BP 807 EP 815 DI 10.1002/mrm.20033 PG 9 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 808GI UT WOS:000220557200021 PM 15065255 ER PT J AU Abe, K Yuzuriha, M Sugimoto, M Ko, MSH Brathwaite, M Waeltz, P Nagaraja, R AF Abe, K Yuzuriha, M Sugimoto, M Ko, MSH Brathwaite, M Waeltz, P Nagaraja, R TI Gene content of the 750-kb critical region for mouse embryonic ectoderm lethal tcl-w5 SO MAMMALIAN GENOME LA English DT Article ID MAJOR HISTOCOMPATIBILITY COMPLEX; CLASS-II REGION; H-2K REGION; PROTEIN; EXPRESSION; SEQUENCES; IDENTIFICATION; RECOMBINATION; MICE; MHC AB Mice homozygous for the t(w5) allele arrest at gastrulation from defects associated with embryonic ectoderm development. The mutated gene has been genetically closely linked to the H-2K locus in the mouse MHC region, flanked by markers H-2Pb and D17Mit147. Aiming at the positional cloning of the mutated gene, we constructed a BAC contig spanning about 1 Mb of the genomic region. On the basis of our mapping and sequencing analysis. of the BACS combined with public genome data, EST database searches, and gene prediction programs, we delimit the 1.06 cM of the t(w5) critical region to 750 kb, and infer 36 genes (1/20 kb) encoded in the interval. All of the 33 genes tested were confirmed as expressed in embryonic tissues by RT-PCR analyses, and in many cases by EST expression profiles as well. Thus, this highly gene-rich region is essentially totally transcribed during early development and provides priority candidates to be screened for the t(w5) embryonic lesion. C1 RIKEN, Tsukuba Inst, BioResource Ctr, Technol & Dev Team Mammalian Cellular Dynam, Tsukuba, Ibaraki 3050074, Japan. Kumamoto Univ, Inst Mol Embryol & Genet, Kumamoto 8620976, Japan. NIA, Genet Lab, NIH, Baltimore, MD 21224 USA. RP Abe, K (reprint author), RIKEN, Tsukuba Inst, BioResource Ctr, Technol & Dev Team Mammalian Cellular Dynam, 3-1-1 Koyadai, Tsukuba, Ibaraki 3050074, Japan. EM abe@rtc.riken.go.jp RI Ko, Minoru/B-7969-2009 OI Ko, Minoru/0000-0002-3530-3015 NR 39 TC 4 Z9 11 U1 0 U2 1 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0938-8990 J9 MAMM GENOME JI Mamm. Genome PD APR PY 2004 VL 15 IS 4 BP 265 EP 276 DI 10.1007/s00335-003-2329-1 PG 12 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 810QV UT WOS:000220719700003 PM 15112104 ER PT J AU Nojima, H Shimizu, T Kim, CH Yabe, T Bae, YK Muraoka, O Hirata, T Chitnis, A Hirano, T Hibi, M AF Nojima, H Shimizu, T Kim, CH Yabe, T Bae, YK Muraoka, O Hirata, T Chitnis, A Hirano, T Hibi, M TI Genetic evidence for involvement of maternally derived Wnt canonical signaling in dorsal determination in zebrafish SO MECHANISMS OF DEVELOPMENT LA English DT Article DE maternal-effect mutant; zebrafish; wnt signaling; organizer ID SYNTHASE KINASE 3-BETA; NODAL-RELATED GENES; BETA-CATENIN; XENOPUS EMBRYOS; AXIS FORMATION; ORGANIZER FORMATION; CORTICAL ROTATION; VENTRAL AXIS; MESODERM FORMATION; HOMEOBOX GENE AB In zebrafish, the program for dorsal specification begins soon after fertilization. Dorsal determinants are localized initially to the vegetal pole, then transported to the blastoderm, where they are thought to activate the canonical Writ pathway, which induces the expression of dorsal-specific genes. We identified a novel maternal-effect recessive mutation, tokkaebi (tkk), that affects formation of the dorsal axis. Severely ventralized phenotypes, including a lack of dorso-anterior structures, were seen in 5- 100% of the embryos obtained from tkk homozygous transmitting females. tkk embryos displayed defects in the nuclear accumulation of beta-catenin on the dorsal side, and reduced or absent expression of dorsal-specific genes. Mesoderm and endoderm formation outside the dorsal axis was not significantly affected. Injection of RNAs for activated beta-catenin, dominant-negative forms of Axin1 and GSK3beta, and wild-type Dv13, into the tkk embryos suppressed the ventralized phenotypes and/or dorsalized the embryos, and restored or induced an ectopic and expanded expression of bozozok/dharma and goosecoid. However, dorsalization by wnt RNAs was affected in the tkk embryos. Inhibition of cytoplasmic calcium release elicited an ectopic and expanded expression of chordin in the wild-type, but did not restore chordin expression efficiently in the tkk embryos. These data indicate that the tkk gene product functions upstream of or parallel to the beta-catenin-degradation machinery to control the stability of beta-catenin. The tkk locus was mapped to chromosome 16. These data provide genetic evidence that the maternally derived canonical Writ pathway upstream of beta-catenin is involved in dorsal axis formation in zebrafish. (C) 2004 Elsevier Ireland Ltd. All rights reserved. C1 Osaka Univ, Grad Sch Med, Dept Mol Oncol, Suita, Osaka 5650871, Japan. Chungnam Natl Univ, Dept Biol, Taejon 305764, South Korea. NICHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. EM shimizu@cdb.riken.jp; hibi@cdb.riken.jp RI Hirano, Toshio/C-8194-2009 NR 83 TC 27 Z9 33 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-4773 J9 MECH DEVELOP JI Mech. Dev. PD APR PY 2004 VL 121 IS 4 BP 371 EP 386 DI 10.1016/j.mod.2004.02.003 PG 16 WC Developmental Biology SC Developmental Biology GA 823AR UT WOS:000221582600006 PM 15110047 ER PT J AU Calvi, LM Shin, HI Knight, MC Weber, JM Young, MF Giovannetti, A Schipani, E AF Calvi, LM Shin, HI Knight, MC Weber, JM Young, MF Giovannetti, A Schipani, E TI Constitutively active PTH/PTHrP receptor in odontoblasts alters odontoblast and ameloblast function and maturation SO MECHANISMS OF DEVELOPMENT LA English DT Article DE parathyroid hormone; PTH-related protein; PTH/PTHrP receptor; odontoblast; ameloblast; cytodifferentiation; Sonic Hedgehog ID HORMONE-RELATED-PROTEIN; ENDOCHONDRAL BONE-FORMATION; PARATHYROID-HORMONE; TOOTH DEVELOPMENT; TRANSGENIC MICE; OSTEOBLASTIC CELLS; DENTAL DEVELOPMENT; ENAMEL KNOT; MOUSE TOOTH; IN-VITRO AB Parathyroid hormone (PTH)-related protein (PTH-rP) is an important autocrine/paracrine attenuator of programmed cell differentiation whose expression is restricted to the epithelial layer in tooth development. The PTH/PTHrP receptor (PPR) mRNA in contrast is detected in the dental papilla, suggesting that PTHrP and the PPR may modulate epithelial-mesenchymal interactions. To explore the possible interactions, we studied the previously described transgenic mice in which a constitutively active PPR is targeted to osteoblastic cells. These transgenic mice have a vivid postnatal bone and tooth phenotype, with normal tooth eruption but abnormal, widened crowns. Transgene mRNA expression was first detected at birth in the dental papilla and, at 1 week postnatally, in odontoblasts. There was no transgene expression in ameloblasts or in other epithelial structures. Prenatally, transgenic molars and incisors revealed no remarkable change. By the age of 1 week, the dental papilla was widened, with disorganization of the odontoblastic layer and decreased dentin matrix. In addition, the number of cusps was abnormally increased, the ameloblastic layer disorganized, and enamel matrix decreased. Odontoblastic and, surprisingly, ameloblastic cytodifferentiation was impaired, as shown by in situ hybridization and electron microscopy. Interestingly, ameloblastic expression of Sonic Hedgehog, a major determinant of ameloblastic cytodifferentiation, was dramatically altered in the transgenic molars. These data suggest that odontoblastic activation of the PPR may play an important role in terminal odontoblastic and, indirectly, ameloblastic cytodifferentiation, and describe a useful model to study how this novel action of the PPR may modulate mesenchymal/epithelial interactions at later stages of tooth morphogenesis and development. (C) 2004 Elsevier Ireland Ltd. All rights reserved. C1 Massachusetts Gen Hosp, Endocrine Unit, Boston, MA 02114 USA. Harvard Univ, Sch Med, Boston, MA USA. Univ Rochester, Sch Med, Dept Med, Endocrine Unit, Rochester, NY USA. Kyungpook Natl Univ, Sch Dent, Dept Oral Pathol, Taegu 702701, South Korea. NIDCR, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD USA. RP Schipani, E (reprint author), Massachusetts Gen Hosp, Endocrine Unit, 501 Wellman Bldg, Boston, MA 02114 USA. EM schipani@helix.mgh.harvard.edu OI Calvi, Laura Maria/0000-0001-6969-239X FU NIDDK NIH HHS [K08 DK064381-03, K08 DK64381, K08 DK064381] NR 46 TC 22 Z9 27 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-4773 J9 MECH DEVELOP JI Mech. Dev. PD APR PY 2004 VL 121 IS 4 BP 397 EP 408 DI 10.1016/j.mod.2004.02.004 PG 12 WC Developmental Biology SC Developmental Biology GA 823AR UT WOS:000221582600008 PM 15110049 ER PT J AU Shalev, A Patterson, NB Hirshberg, B Rother, KI Harlan, DM AF Shalev, A Patterson, NB Hirshberg, B Rother, KI Harlan, DM TI Resistin serum levels in type 1 diabetes pre- and post-islet transplantation SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID INSULIN-RESISTANCE; GLUCOSE; OBESITY AB Resistin is a recently described secretory protein produced in adipocytes that is thought to be involved in insulin resistance, diabetes, and inflammation. While resistin can be detected in mouse and human serum, very little is known about the regulation of serum resistin levels, especially in humans. To test whether resistin levels are affected by type 1 diabetes mellitus (T1DM), we measured serum resistin levels in samples from 5 healthy volunteers and 6 patients with T1DM pre- and 3 months post-islet transplantation using a human resistin enzyme immunoassay (EIA). Interestingly, serum resistin levels were significantly higher in T1DM patients before transplantation compared to normal controls, but decreased to normal levels after islet transplantation. Thus, our results suggest that human resistin may be involved in the pathophysiology of T1DM and thereby reveal a heretofore unappreciated aspect of human resistin biology. (C) 2004 Elsevier Inc. All rights reserved. C1 NIDDK, Transplantat & Autoimmun Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD USA. RP Shalev, A (reprint author), Ctr Clin Sci, H4-526,600 Highland Ave, Madison, WI 53792 USA. NR 14 TC 14 Z9 14 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD APR PY 2004 VL 53 IS 4 BP 403 EP 404 DI 10.1016/j.metabol.2003.11.014 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 808PR UT WOS:000220581500001 PM 15045683 ER PT J AU Moran, SA Patten, N Young, JR Cochran, E Sebring, N Reynolds, J Premkumar, A Depaoli, AM Skarulis, MC Oral, EA Gorden, P AF Moran, SA Patten, N Young, JR Cochran, E Sebring, N Reynolds, J Premkumar, A Depaoli, AM Skarulis, MC Oral, EA Gorden, P TI Changes in body composition in patients with severe lipodystrophy after leptin replacement therapy SO METABOLISM-CLINICAL AND EXPERIMENTAL LA English DT Article ID REVERSES INSULIN-RESISTANCE; GENERALIZED LIPODYSTROPHY; RECOMBINANT LEPTIN; DIABETES-MELLITUS; ADIPOSE-TISSUE; NORMAL-WEIGHT; OBESE; MICE; DEFICIENCY; STERILITY AB Leptin, an adipocyte hormone, when replaced in patients with lipodystrophy, improves insulin resistance, hyperglycemia, dyslipidemia, and hepatic steatosis. Changes in body composition accompany this metabolic improvement. We studied 14 patients (3 men and 11 women); 12 of who had generalized lipodystrophy (7 congenital, 5 acquired), and 2 patients had partial lipodystrophy. Body composition and related parameters were evaluated at baseline and after 4 and 12 months of leptin therapy. Baseline body mass index (BMI) was 21.7 +/- 0.8 kg/m(2), the percent body fat was 9.5% +/- 1.6%, and the serum leptin level was 1.7 +/- 0.3 ng/mL. On treatment, serum leptin levels increased by 10-fold. All patients reported a decrease in appetite on therapy. After 4 months, both daily caloric intake and resting energy expenditure (REE) decreased. The liver volume decreased (baseline = 3,055 +/- 281 cm(3); 4 months = 2,433 +/- 243 cm(3), P = .006). Dual energy x-ray absorptiometry (DEXA) demonstrated significant decreases in fat mass (5.4 +/- 0.8 kg to 5.0 +/- 0.8 kg; P = .003) and lean body mass (51.2 +/- 3.2 kg to 48.3 +/- 3.4 kg; P = .003) at 4 months on therapy. There was no impact of leptin therapy on bone mineral content, mineral density, and metabolism. Changes in body composition occurred during the first 4 months of leptin therapy, but then stabilized and were sustained thereafter. (C) 2004 Elsevier Inc. All rights reserved. C1 NIDDK, CEB, NIH, Bethesda, MD 20892 USA. Univ Michigan, Dept Med, Div Endocrinol, Ann Arbor, MI 48109 USA. Amgen Inc, Thousand Oaks, CA USA. NIH, Dept Nutr, Bethesda, MD 20892 USA. NIH, Dept Radiol, Warren G Magnuson Clin Ctr, Bethesda, MD 20892 USA. RP Gorden, P (reprint author), NIDDK, CEB, NIH, Bldg 10,Room 8S235A,10 Ctr Dr,MSC 1770, Bethesda, MD 20892 USA. OI Oral, Elif/0000-0002-9171-1144 NR 33 TC 54 Z9 56 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0026-0495 J9 METABOLISM JI Metab.-Clin. Exp. PD APR PY 2004 VL 53 IS 4 BP 513 EP 519 DI 10.1016/j.metabol.2003.10.019 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 808PR UT WOS:000220581500019 PM 15045701 ER PT J AU Patterson, GH Lippincott-Schwartz, J AF Patterson, GH Lippincott-Schwartz, J TI Selective photolabeling of proteins using photo activatable GFP SO METHODS LA English DT Article ID GREEN-FLUORESCENT PROTEIN; LIVING CELLS; RED; PHOTOACTIVATION AB Today's cell biologists rely on an assortment of advances in microscopy methods to Study the inner workings of cells and tissues. Among these advances are fluorescent proteins which can be used to tag specifically and, in many cases, non-invasively proteins of interest within a living cell. Introduction of DNA encoding the fluorescently tagged protein of interest into a cell readily allows the visualization of the protein's localization and time-lapse imaging allows the movement of the structure or organelle to which the protein is localized to be observed. To monitor the movement of the protein within the population, researchers generally have to highlight a pool of molecules by perturbing the steady-state fluorescence. This perturbation has traditionally been performed by photobleaching the molecules within a selected region of the cell and monitoring the recovery of molecules into this region or the loss of molecules within other regions. Fluorescent proteins are now available, which allow a pool of molecules to be highlighted directly by photoactivation. Here, we discuss the technical aspects for using one of these recently developed photoactivatable fluorescent proteins, PA-GFP. Published by Elsevier Inc. C1 NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. RP Lippincott-Schwartz, J (reprint author), NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. EM lippincj@mail.nih.gov NR 17 TC 75 Z9 78 U1 2 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1046-2023 J9 METHODS JI Methods PD APR PY 2004 VL 32 IS 4 BP 445 EP 450 DI 10.1016/j.ymeth.2003.10.006 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804HB UT WOS:000220288700012 PM 15003607 ER PT J AU Palmer, RJ AF Palmer, RJ TI Peter Hirsch and biofilms: Microbial ecology's role in a "new" field SO MICROBIAL ECOLOGY LA English DT Biographical-Item C1 NIDCR, Oral Biofilm Commun Sect, Oral Infect & Immun Branch, NIH, Bethesda, MD 20892 USA. RP Palmer, RJ (reprint author), NIDCR, Oral Biofilm Commun Sect, Oral Infect & Immun Branch, NIH, Bethesda, MD 20892 USA. EM rjpalmer@dir.nider.nih.gov NR 1 TC 0 Z9 1 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 0095-3628 J9 MICROBIAL ECOL JI Microb. Ecol. PD APR PY 2004 VL 47 IS 3 BP 200 EP 204 DI 10.1007/s00248-003-1039-2 PG 5 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA 833RT UT WOS:000222357300002 PM 14583827 ER PT J AU Stavreva, DA Muller, WG Hager, GL Smith, CL McNally, JG AF Stavreva, DA Muller, WG Hager, GL Smith, CL McNally, JG TI Rapid glucocorticoid receptor exchange at a promoter is coupled to transcription and regulated by chaperones and proteasomes SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID HEAT-SHOCK-PROTEIN; LIVING CELLS; ESTROGEN-RECEPTOR; IN-VIVO; MOLECULAR CHAPERONES; COACTIVATOR COMPLEXES; NATURAL PROMOTER; INHIBITION; UBIQUITIN; CHROMATIN AB Exchange of the glucocorticoid receptor (GR) at promoter target sites provides the only known system in which transcription factor cycling at a promoter is fast, occurring on a time scale of seconds. The mechanism and function of this rapid exchange are unknown. We provide evidence that proteasome activity is required for rapid GR exchange at a promoter. We also show that chaperones, specifically hsp90, stabilize the binding of GR to the promoter, complicating models in which the associated chaperone, p23, has been proposed to induce GR removal. Our results are the first to connect chaperone and proteasome functions in setting the residence time of a transcription factor at a target promoter. Moreover, our results reveal that longer GR residence times are consistently associated with greater transcriptional output, suggesting a new paradigm in which the rate of rapid exchange provides a means to tune transcriptional levels. C1 NCI, Lab Receptor Biol & Gene Express, Canc Res Ctr, Bethesda, MD 20892 USA. NINDS, Light Imaging Facil, Bethesda, MD 20892 USA. RP McNally, JG (reprint author), 41 Lib Dr, Bethesda, MD 20892 USA. EM mcnallyj@exchange.nih.gov NR 46 TC 179 Z9 185 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 7 BP 2682 EP 2697 DI 10.1128/MCB.24.7.2682-2697.2004 PG 16 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 804YK UT WOS:000220333800009 PM 15024059 ER PT J AU Cheutin, T Gorski, SA May, KM Singh, PB Misteli, T AF Cheutin, T Gorski, SA May, KM Singh, PB Misteli, T TI In vivo dynamics of Swi6 in yeast: Evidence for a Stochastic model of heterochromatin SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID MATING-TYPE LOCI; FISSION YEAST; HISTONE H3; HP1 PROTEINS; SCHIZOSACCHAROMYCES-POMBE; CHROMATIN-STRUCTURE; MAMMALIAN-CELLS; GENE-EXPRESSION; SHADOW DOMAIN; BINDING-SITE AB The mechanism for transcriptional silencing of pericentric heterochromatin is conserved from fission yeast to mammals. Silenced genome regions are marked by epigenetic methylation of histone H3, which serves as a binding site for structural heterochromatin proteins. In the fission yeast Schizosaccharomyces pombe, the major structural heterochromatin protein is Swi6. To gain insight into Swi6 function in vivo, we have studied its dynamics in the nucleus of living yeast. We demonstrate that, in contrast to mammalian cells, yeast heterochromatin domains undergo rapid, large-scale motions within the nucleus. Similar to the situation in mammalian cells, Swi6 does not permanently associate with these chromatin domains but binds only transiently to euchromatin and heterochromatin. Swi6 binding dynamics are dependent on growth status and on the silencing factors Clr4 and Rik1, but not Clr1, Clr2, or Clr3. By comparing the kinetics of mutant Swi6 proteins in swi6(-) and swi6(+) strains, we demonstrate that homotypic protein-protein interactions via the chromoshadow domain stabilize Swi6 binding to chromatin in vivo. Kinetic modeling allowed quantitative estimation of residence times and indicated the existence of at least two kinetically distinct populations of Swi6 in heterochromatin. The observed dynamics of Swi6 binding are consistent with a stochastic model of heterochromatin and indicate evolutionary conservation of heterochromatin protein binding properties from mammals to yeast. C1 NCI, NIH, Bethesda, MD 20892 USA. Roslin Inst, Nucl Reprogamming Lab, Roslin EH25 9PS, Midlothian, Scotland. RP NCI, NIH, Bethesda, MD 20892 USA. EM mistelit@mail.nih.gov RI Singh, Prim/G-1088-2014 NR 56 TC 54 Z9 54 U1 1 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 EI 1098-5549 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3157 EP 3167 DI 10.1128/MCB.24.8.3157-3167.2004 PG 11 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400010 PM 15060140 ER PT J AU Wessells, J Yakar, S Johnson, PF AF Wessells, J Yakar, S Johnson, PF TI Critical prosurvival roles for C/EBP beta and insulin-like growth factor I in macrophage tumor cells SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID BINDING-PROTEIN-BETA; TRANSCRIPTIONAL ACTIVATOR PROTEIN; MOUSE EMBRYO FIBROBLASTS; FRESH BONE-MARROW; PHOSPHATIDYLINOSITOL 3-KINASE; HEMATOPOIETIC-CELLS; AUTOCRINE GROWTH; HEPATOCYTE PROLIFERATION; RECOMBINANT RETROVIRUS; CYTOKINE-DEPENDENCY AB One of the hallmarks of leukemic cells is their ability to proliferate and survive in the absence of exogenous growth factors (GFs). However, the molecular mechanisms used by myeloid tumor cells to escape apoptosis are not fully understood. Here we report that Myc/Raf- transformed macrophages require the transcription factor C/EBPbeta to prevent cell death. In contrast to wild-type cells, C/EBPbeta(-/-) macrophages were completely dependent on macrophage colony-stimulating factor or granulocyte-macrophage colony-stimulating factor for survival and displayed impaired tumorigenicity in vivo. Microarray analysis revealed that C/EBPbeta-deficient cells expressed significantly reduced levels of the prosurvival factor insulin-like growth factor I (IGF-I). Overexpression of C/EBPbeta stimulated transcription from the IGF-I promoter, indicating that IGF-I is a direct transcriptional target of C/EBPP. Serological neutralization of IGF-I in C/EBPbeta(+/+) tumor cell cultures induced apoptosis, showing that IGF-I functions as an autocrine survival factor in these cells. Macrophage tumor cells derived from IGF-I-/- mice were GF dependent, similar to C/EBPO-deficient cells. Forced expression of either C/EBPP or IGF-I in C/EBPbeta(-/-) bone marrow cells restored Myc/Raf-induced transformation and permitted neoplastic growth without exogenous GFs. Thus, our findings demonstrate that C/EBPD is essential for oncogenic transformation of macrophages and functions at least in part by regulating expression of the survival factor IGF-I. C1 NCI, Eukaryot Transcript Regulat Sect, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA. NIDDKD, Diabet Branch, NIH, Bethesda, MD 20892 USA. RP Johnson, PF (reprint author), NCI, Eukaryot Transcript Regulat Sect, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA. EM johnsopf@ncifcrf.gov RI Johnson, Peter/A-1940-2012 OI Johnson, Peter/0000-0002-4145-4725 NR 71 TC 37 Z9 37 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3238 EP 3250 DI 10.1128/MCB.24.8.3238-3250.2004 PG 13 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400017 PM 15060147 ER PT J AU Tomita, A Buchholz, DR Shi, YB AF Tomita, A Buchholz, DR Shi, YB TI Recruitment of N-CoR/SMRT-TBLR1 corepressor complex by unliganded thyroid hormone receptor for gene repression during frog development SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID NUCLEAR RECEPTOR; N-COR; XENOPUS-LAEVIS; TRANSCRIPTIONAL REPRESSION; HISTONE DEACETYLASE; IN-VIVO; AMPHIBIAN METAMORPHOSIS; POSTNATAL-DEVELOPMENT; BETA GENE; TR-BETA AB The corepressors N-CoR (nuclear receptor corepressor) and SMRT (silencing mediator for retinoid and thyroid hormone receptors) interact with unliganded nuclear hormone receptors, including thyroid hormone (T-3) receptor (TR). Several N-CoR/SMRT complexes containing histone deacetylases have been purified. The best studied among them are N-CoR/SMRT complexes containing TBL1 (transducin beta-like protein 1) or TBLR1 (TBL1-related protein). Despite extensive studies of these complexes, there has been no direct in vivo evidence for the interaction of TBL1 or TBLR1 with TR or the possible involvement of such complexes in gene repression by any nuclear receptors in any animals. Here, we used the frog oocyte system to demonstrate that unliganded TR interacts with TBLR1 and recruits TBLR1 to its chromatinized target promoter in vivo, accompanied by histone deacetylation and gene repression. We further provide evidence to show that the recruitment of TBLR1 or related proteins is important for repression by unliganded TR. To investigate the potential role for TBLR1 complexes during vertebrate development, we made use of T-3-dependent amphibian metamorphosis as a model. We found that TBLR1, SMRT, and N-CoR are recruited to T-3-inducible promoters in premetamorphic tadpoles and are released upon T3 treatment, which induces metamorphosis. More importantly, we demonstrate that the dissociation of N-CoR/SMRT-TBLR1 complexes from endogenous TR target promoters is correlated with the activation of these genes during spontaneous metamorphosis. Taken together, our studies provide in vivo evidence for targeted recruitment of N-CoR/SMRT-TBLR1 complexes by unliganded TR in transcriptional repression during vertebrate development. C1 NICHD, Sect Mol Morphogenesis, Lab Gene Regulat & Dev, NIH, Bethesda, MD 20892 USA. RP Shi, YB (reprint author), NICHD, Sect Mol Morphogenesis, Lab Gene Regulat & Dev, NIH, Bldg 18T,Room 106, Bethesda, MD 20892 USA. EM shi@helix.nih-.gov NR 81 TC 71 Z9 73 U1 1 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3337 EP 3346 DI 10.1128/MCB.24.8.3337-3346.2004 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400025 PM 15060155 ER PT J AU Trotter, KW Archer, TK AF Trotter, KW Archer, TK TI Reconstitution of glucocorticoid receptor-dependent transcription in vivo SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID CHROMATIN-REMODELING COMPLEX; HUMAN BREAST-CANCER; ORDERED RECRUITMENT; SWI-SNF; PROGESTERONE-RECEPTOR; NUCLEAR RECEPTORS; ACTIVATOR BINDING; NUCLEOSOMAL DNA; GENE-EXPRESSION; SWI/SNF FAMILY AB We developed a model system to study glucocorticoid receptor (GR)-mediated chromatin remodeling by the BRG1 complex. Introduction of the BRG1 ATPase into the SW-13 cell line initiates the formation of a functional remodeling complex. This complex is able to induce transcriptional activation from a transiently transfected promoter with wild-type and chromatin-remodeling-deficient BRG1 mutants, suggesting that the complex possesses a coactivator function independent from remodeling. Transactivation from a chromatin template requires the BRG1 remodeling function, which induces regions of hypersensitivity and transcription factor loading onto the integrated MMTV promoter. We report that BRG1 remodeling activity is required for GR-mediated transactivation and that this activity cannot be replaced by other ATP-dependent remodeling proteins. Further characterization of the BRG1-associated factors (BAFs) present in these cells (for example, the expression of BAF250 but not BAF180) reveals that the BAF complex rather than the polybromo-associated BAF complex is the necessary and sufficient chromatin-remodeling component with which the receptor functions in vivo. These results in conjunction with previous findings demonstrate that the GR functions with multiple forms of the SWI/SNF complex in vivo. C1 NIEHS, Mol Carcinogenesis Lab, Chromatin & Express Sect, NIH, Res Triangle Pk, NC 27709 USA. RP Archer, TK (reprint author), NIEHS, Mol Carcinogenesis Lab, Chromatin & Express Sect, NIH, POB 12233,111 TW Alexander Dr,MD E-4-06, Res Triangle Pk, NC 27709 USA. EM archer1@niehs.nih.gov NR 69 TC 74 Z9 75 U1 0 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3347 EP 3358 DI 10.1128/MCB.24.8.3347-3358.2004 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400026 PM 15060156 ER PT J AU Wang, LX Lin, CM Brooks, S Cimbora, D Groudine, M Aladjem, MI AF Wang, LX Lin, CM Brooks, S Cimbora, D Groudine, M Aladjem, MI TI The human beta-globin replication initiation region consists of two modular independent replicators SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID BIDIRECTIONAL DNA-REPLICATION; DIHYDROFOLATE-REDUCTASE ORIGIN; BINDING-SITES; SCHIZOSACCHAROMYCES-POMBE; SACCHAROMYCES-CEREVISIAE; RECOGNITION COMPLEX; UNWINDING ELEMENT; SEQUENCE ELEMENTS; INVERTED REPEATS; NUCLEAR MATRIX AB Previous studies have shown that mammalian cells contain replicator sequences, which can determine where DNA replication initiates. However, the specific sequences that confer replicator activity were not identified. Here we report a detailed analysis of replicator sequences that dictate initiation of DNA replication from the human beta-globin locus. This analysis suggests that the beta-globin replication initiation region contains two adjacent, redundant replicators. Each replicator was capable of initiating DNA replication independently at ectopic sites. Within each of these two replicators, we identified short, discrete, nonredundant sequences, which cooperatively determine replicator activity. Experiments with somatic cell hybrids further demonstrated that the requirements for initiation at ectopic sites were similar to the requirements for initiation within native human chromosomes. The replicator clustering and redundancy exemplified in the human beta-globin locus may account for the extreme difficulty in identifying replicator sequences in mammalian cells and suggest that mammalian replication initiation sites may be determined by cooperative sequence modules. C1 NCI, Mol Pharmacol Lab, DBS NCI NIH, Ctr Canc Res, Bethesda, MD 20892 USA. Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. RP Aladjem, MI (reprint author), NCI, Mol Pharmacol Lab, DBS NCI NIH, Ctr Canc Res, Bldg 37,Rm 5056,37 Convent Dr, Bethesda, MD 20892 USA. EM aladjemm@mail.nih.gov RI Aladjem, Mirit/G-2169-2010 OI Aladjem, Mirit/0000-0002-1875-3110 FU NHLBI NIH HHS [HL57620]; NIDDK NIH HHS [DK44746, R37 DK044746] NR 56 TC 47 Z9 47 U1 0 U2 1 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3373 EP 3386 DI 10.1128/MCB.24.8.3373-3386.2004 PG 14 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400028 PM 15060158 ER PT J AU Kazemi, S Papadopoulou, S Li, SY Su, QZ Wang, SO Yoshimura, A Matlashewski, G Dever, TE Koromilas, AE AF Kazemi, S Papadopoulou, S Li, SY Su, QZ Wang, SO Yoshimura, A Matlashewski, G Dever, TE Koromilas, AE TI Control of alpha subunit of eukaryotic translation initiation factor 2 (eIF2 alpha) phosphorylation by the human papillomavirus type 18 E6 oncoprotein: Implications for eIF2 alpha-dependent gene expression and cell death SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID PROTEIN-KINASE PKR; POLYACRYLAMIDE-GEL ELECTROPHORESIS; INTERFERON-INDUCED PKR; NF-KAPPA-B; STRANDED-RNA; ENDOPLASMIC-RETICULUM; MAMMALIAN-CELLS; HOST-DEFENSE; HIGH-RISK; APOPTOSIS AB Phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) at serine 51 inhibits protein synthesis in cells subjected to various forms of stress including virus infection. The human papillomavirus (HPV) E6 oncoprotein contributes to virus-induced pathogenicity through multiple mechanisms including the inhibition of apoptosis and the blockade of interferon (IFN) action. We have investigated a possible functional relationship between the E6 oncoprotein and eIF2alpha phosphorylation by an inducibledimerization form of the IFN-inducible protein kinase PKR. Herein, we demonstrate that HPV type 18 E6 protein synthesis is rapidly repressed upon eIF2alpha phosphorylation caused by the conditional activation of the kinase. The remainder of E6, however, can rescue cells from PKR-mediated inhibition of protein synthesis and induction of apoptosis. E6 physically associates with GADD34/PP1 holophosphatase complex, which mediates translational recovery, and facilitates eIF2a dephosphorylation. Inhibition of eIF2alpha phosphorylation by E6 mitigates eIF2alpha-dependent responses to transcription and translation of proapoptotic genes. These findings demonstrate, for the first time, a role of the oncogenic E6 in apoptotic signaling induced by PKR and eIF2alpha phosphorylation. The functional interaction between E6 and the eIF2a phosphorylation pathway may have important implications for HPV infection and associated pathogenesis. C1 McGill Univ, Lady Davis Inst, Sir Mortimer B Davis Jewish Gen Hosp, Montreal, PQ H3T 1E2, Canada. McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada. Kyushu Univ, Med Inst Bioregulat, Fukuoka 8128582, Japan. NICHD, Lab Gene Regulat & Dev, NIH, Bethesda, MD 20892 USA. RP Koromilas, AE (reprint author), McGill Univ, Lady Davis Inst, Sir Mortimer B Davis Jewish Gen Hosp, 3755 Cote Ste Catherine St, Montreal, PQ H3T 1E2, Canada. EM antonis.koromilas@mcgill.ca RI Yoshimura, Akihiko/K-5515-2013; OI Dever, Thomas/0000-0001-7120-9678 NR 87 TC 59 Z9 67 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3415 EP 3429 DI 10.1128/MCB.24.8.3415-3429.2004 PG 15 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400032 PM 15060162 ER PT J AU Pant, V Kurukuti, S Pugacheva, E Shamsuddin, S Mariano, P Renkawitz, R Klenova, E Lobanenkov, V Ohlsson, R AF Pant, V Kurukuti, S Pugacheva, E Shamsuddin, S Mariano, P Renkawitz, R Klenova, E Lobanenkov, V Ohlsson, R TI Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritance SO MOLECULAR AND CELLULAR BIOLOGY LA English DT Article ID ENHANCER-BLOCKING ACTIVITY; MOUSE H19; PROTEIN CTCF; GENE; EXPRESSION; INSULATOR; LOCUS; ASSOCIATION; PHENOTYPES; SEQUENCES AB The differentially methylated imprinting control region (ICR) region upstream of the H19 gene regulates allelic Igf2 expression by means of a methylation-sensitive chromatin insulator function. We have previously shown that maternal inheritance of mutated (three of the four) target sites for the 11-zinc finger protein CTCF leads to loss of Igf2 imprinting. Here we show that a mutation in only CTCF site 4 also leads to robust activation of the maternal Igf2 allele despite a noticeably weaker interaction in vitro of site 4 DNA with CTCF compared to other ICR sites, sites 1 and 3. Moreover, maternally inherited sites I to 3 become de novo methylated in complex patterns in subpopulations of liver and heart cells with a mutated site 4, suggesting that the methylation privilege status of the maternal H19 ICR allele requires an interdependence between all four CTCF sites. In support of this conclusion, we show that CTCF molecules bind to each other both in vivo and in vitro, and we demonstrate strong interaction between two CTCF-DNA complexes, preassembled in vitro with sites 3 and 4. We propose that the CTCF sites may cooperate to jointly maintain both methylation-free status and insulator properties of the maternal H19 ICR allele. Considering many other CTCF targets, we propose that site-specific interactions between various DNA-bound CTCF molecules may provide general focal points in the organization of looped chromatin domains involved in gene regulation. C1 Uppsala Univ, Dept Genet & Dev, Evolut Biol Ctr, S-75236 Uppsala, Sweden. NIAID, Immunopathol Lab, Mol Pathol Sect, Bethesda, MD 20892 USA. Univ Essex, Dept Biol Sci, Colchester CO4 3SQ, Essex, England. Univ Giessen, Inst Genet, D-35392 Giessen, Germany. RP Ohlsson, R (reprint author), Uppsala Univ, Dept Genet & Dev, Evolut Biol Ctr, Norbyvagen 18A, S-75236 Uppsala, Sweden. EM VLOBANENKOV@niaid.nih.gov; Rolf.Ohlsson@ebc.uu.se RI Shamsuddin, Shaharum/A-7907-2011; OI Shamsuddin, Shaharum/0000-0001-9997-7740; Lobanenkov, Victor/0000-0001-6665-3635 NR 28 TC 104 Z9 107 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0270-7306 J9 MOL CELL BIOL JI Mol. Cell. Biol. PD APR PY 2004 VL 24 IS 8 BP 3497 EP 3504 DI 10.1128/MCB.24.8.3497-3504.2004 PG 8 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 810DQ UT WOS:000220685400038 PM 15060168 ER PT J AU Heron-Milhavet, L Xue-jun, Y Vannucci, SJ Wood, TL Willing, LB Stannard, B Hernandez-Sanchez, C Mobbs, C Virsolvy, A LeRoith, D AF Heron-Milhavet, L Xue-jun, Y Vannucci, SJ Wood, TL Willing, LB Stannard, B Hernandez-Sanchez, C Mobbs, C Virsolvy, A LeRoith, D TI Protection against hypoxic-ischemic injury in transgenic mice overexpressing Kir6.2 channel pore in forebrain SO MOLECULAR AND CELLULAR NEUROSCIENCE LA English DT Article ID K-ATP CHANNELS; SENSITIVE POTASSIUM CHANNELS; SULFONYLUREA RECEPTOR; HIPPOCAMPAL SLICES; GLOBAL-ISCHEMIA; NEURONAL DEATH; BINDING-SITES; RAT-BRAIN; ADENOSINE; SUBUNIT AB The role of the K-ATP channel pore-forming subunit Kir6.2 on protection from cerebral hypoxic-ischemic injury was assessed in transgenic mice overexpressing normal Vir6.2 or a dominant negative form (AFA) of this subunit in the forebrain. The resulting mice overexpress either the Kir6.2 or the AFA transgene mainly in the cerebral cortex and hippocampus. The Kir6.2 transgenic mice are resistant to hypoxic-ischemic injury showing a decreased region of cortical damage as compared to the dominant negative AFA and the wild-type mice. Moreover, the overexpression of Kir6.2 allowed an important silencing of the neurons present in forebrain regions thus protecting them from ischemic injury. Interestingly, the phenotype observed in Kir6.2 transgenic mice was observed without increased sulfonylurea binding. Taken together, these results indicate that the transgenic overexpression of Kir6.2 in forebrain significantly protects mice from hypoxic-ischemic injury and neuronal damage seen in stroke. Published by Elsevier Inc. C1 NIDDK, NIH, Diabet Branch, Bethesda, MD 20892 USA. Mt Sinai Sch Med, Fishberg Ctr Neurobiol, New York, NY 10029 USA. Columbia Univ Coll Phys & Surg, Morgan Stanley Childrens Hosp NY, New York, NY 10032 USA. Penn State Univ, Coll Med, Hershey, PA 17033 USA. CHU A, INSERM, U3906, F-34295 Montpellier, France. RP LeRoith, D (reprint author), NIDDK, NIH, Diabet Branch, Room 8D12,Bldg 10,10 Ctr Dr,MSC 1758, Bethesda, MD 20892 USA. EM Derek@helix.nih.gov RI Hernandez Sanchez, Catalina/N-1737-2014; VIRSOLVY, Anne/E-4964-2016 OI Hernandez Sanchez, Catalina/0000-0002-0846-5019; VIRSOLVY, Anne/0000-0002-5819-3306 NR 38 TC 25 Z9 26 U1 1 U2 2 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1044-7431 J9 MOL CELL NEUROSCI JI Mol. Cell. Neurosci. PD APR PY 2004 VL 25 IS 4 BP 585 EP 593 DI 10.1016/j.mcn.2003.10.012 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 812PW UT WOS:000220852400004 PM 15080888 ER PT J AU Cox, R Mason-Gamer, RJ Jackson, CL Segev, N AF Cox, R Mason-Gamer, RJ Jackson, CL Segev, N TI Phylogenetic analysis of Sec7-domain-containing Arf nucleotide exchangers SO MOLECULAR BIOLOGY OF THE CELL LA English DT Review ID ADP-RIBOSYLATION FACTOR; PLECKSTRIN-HOMOLOGY DOMAINS; GUANINE-NUCLEOTIDE; BREFELDIN-A; SEC7 DOMAIN; ENDOPLASMIC-RETICULUM; PLASMA-MEMBRANE; SACCHAROMYCES-CEREVISIAE; CONTAINING PROTEIN; CONSERVED DOMAIN AB The eukaryotic family of ADP-ribosylation factor (Arf) GTPases plays a key role in the regulation of protein trafficking, and guanine-nucleotide exchange is crucial for Arf function. Exchange is stimulated by members of another family of proteins characterized by a 200-amino acid Sec7 domain, which alone is sufficient to catalyze exchange on Arf. Here, we analyzed the phylogeny of Sec7-domain-containing proteins in seven model organisms, representing fungi, plants, and animals. The phylogenetic tree has seven main groups, of which two include members from all seven model systems. Three groups are specific for animals, whereas two are specific for fungi. Based on this grouping, we propose a phylogenetically consistent set of names for members of the Sec7-domain family. Each group, except for one, contains proteins with known Arf exchange activity, implying that all members of this family have this activity. Contrary to the current convention, the sensitivity of Arf exchange activity to the inhibitor brefeldin A probably cannot be predicted by group membership. Multiple alignment reveals group-specific domains outside the Sec7 domain and a set of highly conserved amino acids within it. Determination of the importance of these conserved elements in Arf exchange activity and other cellular functions is now possible. C1 Univ Illinois, Dept Sci Biol, Mol Biol Lab, Chicago, IL 60607 USA. Univ Illinois, Dept Biochem & Mol Genet, Chicago, IL 60607 USA. Univ Illinois, Dept Biol Sci, Sect Ecol & Evolut, Chicago, IL 60607 USA. NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. RP Univ Illinois, Dept Sci Biol, Mol Biol Lab, Chicago, IL 60607 USA. EM nava@uic.edu RI Jackson, Catherine/A-3421-2013 OI Jackson, Catherine/0000-0002-0843-145X FU NIGMS NIH HHS [GM-45444, R01 GM045444] NR 111 TC 107 Z9 115 U1 0 U2 4 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 EI 1939-4586 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR PY 2004 VL 15 IS 4 BP 1487 EP 1505 DI 10.1091/mbc.E0.-06-0443 PG 19 WC Cell Biology SC Cell Biology GA 806RK UT WOS:000220450800002 PM 14742722 ER PT J AU Park, EK Warner, N Bong, YS Stapleton, D Maeda, R Pawson, T Daar, IO AF Park, EK Warner, N Bong, YS Stapleton, D Maeda, R Pawson, T Daar, IO TI Ectopic EphA4 receptor induces posterior protrusions via FGF signaling in Xenopus embryos SO MOLECULAR BIOLOGY OF THE CELL LA English DT Article ID FIBROBLAST-GROWTH-FACTOR; NEURAL CREST CELLS; TYROSINE KINASE; MESODERM INDUCTION; EPHB2 RECEPTOR; LAEVIS EMBRYOS; SAM DOMAIN; JUXTAMEMBRANE REGION; GENE-EXPRESSION; TAIL BUD AB The Eph family of receptor tyrosine kinases regulates numerous biological processes. To examine the biochemical and developmental contributions of specific structural motifs within Eph receptors, wild-type or mutant forms of the EphA4 receptor were ectopically expressed in developing Xenopus embryos. Wild-type EphA4 and a mutant lacking both the SAM domain and PDZ binding motif were constitutively tyrosine phosphorylated in vivo and catalytically active in vitro. EphA4 induced loss of cell adhesion, ventro-lateral protrusions, and severely expanded posterior structures in Xenopus embryos. Moreover, mutation of a conserved SAM domain tyrosine to phenylalanine (Y928F) enhanced the ability of EphA4 to induce these phenotypes, suggesting that the SAM domain may negatively regulate some aspects of EphA4 activity in Xenopus. Analysis of double mutants revealed that the Y928F EphA4 phenotypes were dependent on kinase activity; juxtamembrane sites of tyrosine phosphorylation and SH2 domain-binding were required for cell dissociation, but not for posterior protrusions. The induction of protrusions and expansion of posterior structures is similar to phenotypic effects observed in Xenopus embryos expressing activated FGFR1. Furthermore, the budding ectopic protrusions induced by EphA4 express FGF-8, FGFR1, and FGFR4a. In addition, antisense morpholino oligonucleotide-mediated loss of FGF-8 expression in vivo substantially reduced the phenotypic effects in EphA4Y928F expressing embryos, suggesting a connection between Eph and FGF signaling. C1 NCI, Regulat Cell Growth Lab, Ft Detrick, MD 21702 USA. Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Programme Mol Biol & Canc, Toronto, ON M5G 1X5, Canada. Univ Toronto, Dept Mol & Med Genet, Toronto, ON M5G 1A8, Canada. RP Daar, IO (reprint author), NCI, Regulat Cell Growth Lab, Ft Detrick, MD 21702 USA. EM daar@ncifcrf.gov RI Pawson, Tony/E-4578-2013; OI Daar, Ira/0000-0003-2657-526X NR 56 TC 28 Z9 29 U1 0 U2 0 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR PY 2004 VL 15 IS 4 BP 1647 EP 1655 DI 10.1091/mbc.E03-09-0674 PG 9 WC Cell Biology SC Cell Biology GA 806RK UT WOS:000220450800015 PM 14742708 ER PT J AU Park, CJ Song, S Giddings, TH Ro, HS Sakchaisri, K Park, JE Seong, YS Winey, M Lee, KS AF Park, CJ Song, S Giddings, TH Ro, HS Sakchaisri, K Park, JE Seong, YS Winey, M Lee, KS TI Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae SO MOLECULAR BIOLOGY OF THE CELL LA English DT Article ID SPINDLE POLE BODY; IN-VITRO REGULATION; PROTEIN-KINASE; ESCHERICHIA-COLI; NUCLEAR-ENVELOPE; SHUTTLE VECTORS; BUDDING YEAST; EXIT NETWORK; TEM1 GTPASE; LOCALIZATION AB The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we report the isolation of Bbp1p as a polo-box interacting protein by a yeast two-hybrid screen. Bbp1p localizes to the periphery of the central plaque of the SPB and plays an important role in SPB duplication. Similarly, Cdc5p localized to the cytoplasmic periphery of the SPB. In vitro binding studies showed that Cdc5p interacted with the N-terminal domain of Bbp1p (BbplpDeltaC), but apparently not with Mps2p, a component shown to form a stable complex with Bbp1p. In addition, Bbp1p, but likely not Mps2p, was required for proper localization of Cdc5p to the SPB. The C-terminal coiled-coil domain of Bbp1p (Bbp1p(243-385)), which is crucial for both the homodimerization and the SPB localization, could target the localization-defective Cdc5pDeltaC to the SPB and induce the release of Cdc14p from the nucleolus. Consistent with this observation, expression of CDC5DeltaC-BBP1(243-385) under CDC5 promoter control partially complemented the cdc5Delta defect. These data suggest that Bbp1pDeltaC interacts with the polo-box domain of Cdc5p, and this interaction is critical for the subcellular localization and mitotic functions of Cdc5p. C1 NCI, Lab Metab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA. RP Lee, KS (reprint author), NCI, Lab Metab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA. EM kyunglee@pop.nci.nih.gov FU NIGMS NIH HHS [R01 GM051312, R01GM-51312] NR 44 TC 9 Z9 10 U1 0 U2 1 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR PY 2004 VL 15 IS 4 BP 1711 EP 1723 DI 10.1091/mbc.E03-07-0461 PG 13 WC Cell Biology SC Cell Biology GA 806RK UT WOS:000220450800021 PM 14767068 ER PT J AU Maruvada, P Dmitrieva, NI East-Palmer, J Yen, PM AF Maruvada, P Dmitrieva, NI East-Palmer, J Yen, PM TI Cell cycle-dependent expression of thyroid hormone receptor-beta is a mechanism for variable hormone sensitivity SO MOLECULAR BIOLOGY OF THE CELL LA English DT Article ID DEOXYRIBONUCLEIC-ACID SYNTHESIS; CULTURED GC CELLS; GLUCOCORTICOID-RECEPTOR; HYPERTONIC STRESS; GENE-EXPRESSION; TRANSCRIPTIONAL ACTIVITY; AMPHIBIAN METAMORPHOSIS; ESTROGEN-RECEPTOR; NUCLEAR RECEPTORS; PITUITARY-TUMOR AB Thyroid hormone receptors (TRs) are ligand-regulatable transcription factors. Currently, little is known about the expression of TRs or other nuclear hormone receptors during the cell cycle. We thus developed a stable expression system to express green fluorescent protein-TRbeta in HeLa cells under tetracycline regulation, and studied TR expression during the cell cycle by laser scanning cytometry. Only similar to9-15% of the nonsynchronized cell population expressed TR because the majority of cells were in G(1) phase and did not express detectable amounts of TR. However, when cells were synchronized in early S phase with hydroxyurea and then released, TR expression levels increased in a cell cycle-dependent manner and peaked to 30-40% cells expressing TR at late G(2)/M phase before declining to nonsynchronized levels. Moreover, we observed a direct correlation between transcriptional activity and TR expression during the cell cycle. Similar cell cycle-dependent findings also were observed for endogenous TR in rat pituitary GH(3) cells. Last, cycloheximide studies demonstrated that the increase in TR expression was primarily due to increased translation. These novel observations of cell cycle-dependent expression of TR suggest that differential hormone sensitivity can occur during the cell cycle and may contribute to cell cycle progression during normal development and oncogenesis. C1 NIDDKD, Mol Regulat & Neuroendocrinol Sect, Clin Endocrinol Branch, Bethesda, MD 20892 USA. NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bethesda, MD 20892 USA. RP Yen, PM (reprint author), Johns Hopkins Bayview Med Ctr, Div Endocrinol, NIH, 4940 Eastern Ave,B114, Baltimore, MD 21224 USA. EM pyen3@jhm.edu RI Dmitrieva, Natalia/A-2924-2013 OI Dmitrieva, Natalia/0000-0001-8074-6950 NR 50 TC 17 Z9 18 U1 0 U2 1 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 J9 MOL BIOL CELL JI Mol. Biol. Cell PD APR PY 2004 VL 15 IS 4 BP 1895 EP 1903 DI 10.1091/mbc.E03-09-0636 PG 9 WC Cell Biology SC Cell Biology GA 806RK UT WOS:000220450800037 PM 14767065 ER PT J AU Park, JK Chung, YM Kim, BG Yoo, YA Yang, BS Kim, JS Yoo, YD AF Park, JK Chung, YM Kim, BG Yoo, YA Yang, BS Kim, JS Yoo, YD TI N'-(phenyl-pyridin-2-yl-methylene)-hydrazine carbodithioic acid methyl ester enhances radiation-induced cell death by targeting Bcl-2 against human lung carcinoma cells SO MOLECULAR CANCER THERAPEUTICS LA English DT Article ID CANCER-CELLS; IONIZING-RADIATION; INDUCED APOPTOSIS; P53; KINASE; ACTIVATION; INVOLVEMENT; WORTMANNIN; INHIBITION; RESISTANCE AB To develop a new radiosensitizer, we screened a chemical library and selected one chemical reagent, N'-(phenyl-pyridin-2-yl-methylene)-hydrazine carbodithioic acid methyl ester (PHCM), which was already known to have antifungal and antimicrobial properties. PHCM enhanced radiation-induced cell death and its mean calculated dose enhancement ratio was 1.17. PHCM was found to induce the phosphorylation of p38 mitogen-activated protein kinase, and combined treatment with PHCM and radiation down-regulated Bcl-2. In a xenograft assay, the combined PHCM and radiation group showed 39.3 days of growth delay versus the control in terms of tumor growth. The enhancement factor of this combined treatment was determined to be 4.02. C1 Korea Univ, Inst Canc, Coll Med, Genom Res Ctr,Sungbuk Ku, Seoul 136705, South Korea. Korea Univ, Inst Canc, Coll Med, Brain Korea Biomed Sci 21, Seoul 136705, South Korea. Korea Univ, Inst Canc, Coll Med, Dept Internal Med, Seoul 136705, South Korea. Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea. NCI, Lab Cell Regulat & Carcinogenesis, NIH, Bethesda, MD 20892 USA. Korea Inst Sci & Technol, Div Life Sci, Seoul 130650, South Korea. RP Yoo, YD (reprint author), Korea Univ, Inst Canc, Coll Med, Genom Res Ctr,Sungbuk Ku, 126-1,5KA,Anam Dong, Seoul 136705, South Korea. EM ydy1320@yahoo.co.kr NR 30 TC 6 Z9 7 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1535-7163 J9 MOL CANCER THER JI Mol. Cancer Ther. PD APR PY 2004 VL 3 IS 4 BP 403 EP 407 PG 5 WC Oncology SC Oncology GA 813MU UT WOS:000220912000003 PM 15078983 ER PT J AU Camphausen, K Brady, KJ Burgan, WE Cerra, MA Russell, JS Bull, EEA Tofilon, PJ AF Camphausen, K Brady, KJ Burgan, WE Cerra, MA Russell, JS Bull, EEA Tofilon, PJ TI Flavopiridol enhances human tumor cell radiosensitivity and prolongs expression of gamma H2AX foci SO MOLECULAR CANCER THERAPEUTICS LA English DT Article ID DEPENDENT KINASE INHIBITOR; PHOSPHORYLATED HISTONE H2AX; BREAST-CARCINOMA CELLS; PHASE-I; DOWN-REGULATION; CANCER-CELLS; P-TEFB; TRANSCRIPTIONAL REPRESSION; IONIZING IRRADIATION; SOLID TUMORS AB Flavopiridol is a cyclin-dependent kinase (CDK) inhibitor, which has recently entered clinical trials. However, when administered as a single agent against solid tumors, the antitumor actions of flavopiridol have been primarily cytostatic. Given its reported effects on cell cycle regulation, transcription, and apoptosis, flavopiridol may also influence cellular radioresponse. Thus, to evaluate the potential for combining this cyclin-dependent kinase inhibitor with radiation as a cancer treatment strategy, we have investigated the effects of flavopiridol on the radiation sensitivity of two human prostate cancer cell lines (DU145 and PC3). The data presented here indicate that exposure to flavopiridol (60-90 nm) after irradiation enhanced the radiosensitivity of both DU145 and PC3 cells. This sensitization occurred in the absence of significant reductions in cell proliferation, retinoblastoma protein phosphorylation, or P-TEFb activity. Moreover, the post-irradiation addition of flavopiridol had no effect on radiation-induced apoptosis or the activation of the G(2) cell cycle checkpoint. However, flavopiridol did modify the time course of gammaH2AX expression in irradiated cells. Whereas there was no significant difference in radiation-induced gammaH2AX foci at 6 h, at 24 h after irradiation, the number of cells expressing gammaH2AX foci was significantly greater in the flavopiridol-treated cells. These results indicate that flavopiridol can enhance radiosensitivity of human tumor cells and suggest that this effect may involve an inhibition of DNA repair. C1 NCI, Radiat Oncol Branch, Bethesda, MD 20892 USA. NCI, Mol Radiat Therapeut Branch, Bethesda, MD 20892 USA. RP Tofilon, PJ (reprint author), Radiat Oncol Sci Program, Mol Radiat Therapeut Branch, EPN-6015A,6130 Execut Blvd,MSC 7440, Rockville, MD 20852 USA. EM tofilonp@mail.nih.gov NR 39 TC 28 Z9 29 U1 0 U2 1 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1535-7163 J9 MOL CANCER THER JI Mol. Cancer Ther. PD APR PY 2004 VL 3 IS 4 BP 409 EP 416 PG 8 WC Oncology SC Oncology GA 813MU UT WOS:000220912000004 PM 15078984 ER PT J AU Citrin, D Lee, AK Scott, T Sproull, M Menard, C Tofilon, PJ Camphausen, K AF Citrin, D Lee, AK Scott, T Sproull, M Menard, C Tofilon, PJ Camphausen, K TI In vivo tumor imaging in mice with near-infrared labeled endostatin SO MOLECULAR CANCER THERAPEUTICS LA English DT Article ID ENDOTHELIAL-CELL-PROLIFERATION; ANGIOGENESIS INHIBITOR; CARCINOMA; METASTASES; GROWTH; CANCER AB Endostatin is a potent inhibitor of angiogenesis currently in phase I clinical trials. Imaging technologies that use near infrared fluorescent probes are well suited to the laboratory setting. The goal of this study was to determine whether endostatin labeled with a near-infrared probe (Cy5.5) could be detected in an animal and whether it would selectively localize to a tumor. Endostatin was conjugated to Cy5.5 monofunctional dye and injected into mice bearing Lewis lung carcinoma tumors (350 mm(2)). Mice were imaged at various time points while under sedation using a lightproof box affixed to a fluorescent microscope mounted with a filter in the near-infrared bandwidth consistent with Cy5.5 fluorescence. After i.p. injection, endostatin-Cy5.5 was absorbed producing a near-infrared fluorescent image within the tumors at 18 h reaching a maximum at 42 h after injection. No signal was emitted from mice injected with unlabeled endostatin or Cy5.5 dye alone or those that received no injection. Further results show that a dose response exists with injection of endostatin-Cy5.5. Mimicking the clinical route of administration, an i.v. injection had a peak signal emission at 3 h but also persisted to 72 h. Finally, to determine the intratumoral binding site for endostatin, we performed immunofluorescence on tumor specimens and demonstrated that endostatin binds to tumor vasculature and colocalizes with platelet/endothelial cell adhesion molecule 1 expression. This study demonstrates that endostatin covalently bound to Cy5.5 will migrate from a distant i.p. injection site to a tumor. These data indicate that endostatin-Cy5.5 is appropriate for selectively imaging tumors in uninjured experimental animals. C1 NCI, Radiat Oncol Branch, Imaging & Mol Therapeut Sect, Bethesda, MD 20892 USA. NCI, Vasc Biol Fac, Bethesda, MD 20892 USA. NCI, Mol Radiat Therapeut Branch, Bethesda, MD 20892 USA. Univ Texas, MD Anderson Canc Ctr, Dept Radiat Oncol, Houston, TX 77030 USA. RP Camphausen, K (reprint author), NCI, Radiat Oncol Branch, Imaging & Mol Therapeut Sect, 10 Ctr Dr,Bldg 10,Room B3B69, Bethesda, MD 20892 USA. EM camphauk@mail.nih.gov NR 28 TC 40 Z9 40 U1 2 U2 11 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 1535-7163 J9 MOL CANCER THER JI Mol. Cancer Ther. PD APR PY 2004 VL 3 IS 4 BP 481 EP 488 PG 8 WC Oncology SC Oncology GA 813MU UT WOS:000220912000012 PM 15078992 ER PT J AU Frijhoff, AFW Conti, CJ Senderowicz, AM AF Frijhoff, AFW Conti, CJ Senderowicz, AM TI Advances in molecular carcinogenesis: Current and future use of mouse models to screen and validate molecularly targeted anticancer drugs SO MOLECULAR CARCINOGENESIS LA English DT Review DE genetically engineered mice; human tumor development; conditional gene targeting; Cre/loxP; clinical trials ID FARNESYL-PROTEIN TRANSFERASE; ADVANCED SOLID TUMORS; NUCLEAR RECEPTOR SXR; RAS TRANSGENIC MICE; HUMAN BREAST-CANCER; PHASE-II TRIAL; IN-VIVO; FARNESYLTRANSFERASE INHIBITORS; ANTITUMOR-ACTIVITY; CRE RECOMBINASE AB Survival of patients with advanced solid tumors has not significantly improved over the past 30 years. Although molecularly targeted anticancer drugs offer promise, few drugs make it through the end of the Food and Drug Administration approval process. Animal models that more closely resemble human carcinogenesis may bridge the gap between preclinical success and benefits for patients. We discuss pros and cons of several mouse models, including genetically engineered mice that each represent different aspects of human cancer, and the screening of targeted drugs in these models. Published 2004 Wiley-Liss, Inc. C1 Natl Inst Dent & Craniofacial Res, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD 20892 USA. Univ Texas, MD Anderson Canc Ctr, Div Sci Pk Res, Smithville, TX USA. Univ Texas, Grad Sch Biomed Sci, Program Environm & Mol Carcinogenesis, Houston, TX 77025 USA. RP Senderowicz, AM (reprint author), Natl Inst Dent & Craniofacial Res, Oral & Pharyngeal Canc Branch, NIH, Bldg 30,Room 211,30 Convent Dr, Bethesda, MD 20892 USA. FU NCI NIH HHS [CA42157, CA76540, CA90922]; NIEHS NIH HHS [ES07784] NR 86 TC 9 Z9 10 U1 0 U2 2 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0899-1987 J9 MOL CARCINOGEN JI Mol. Carcinog. PD APR PY 2004 VL 39 IS 4 BP 183 EP 194 DI 10.1002/mc.20013 PG 12 WC Biochemistry & Molecular Biology; Oncology SC Biochemistry & Molecular Biology; Oncology GA 808ZD UT WOS:000220606100001 PM 15057870 ER PT J AU Anderson, NL Polanski, M Pieper, R Gatlin, T Tirumalai, RS Conrads, TP Veenstra, TD Adkins, JN Pounds, JG Fagan, R Lobley, A AF Anderson, NL Polanski, M Pieper, R Gatlin, T Tirumalai, RS Conrads, TP Veenstra, TD Adkins, JN Pounds, JG Fagan, R Lobley, A TI The human plasma proteome - A nonredundant list developed by combination of four separate sources SO MOLECULAR & CELLULAR PROTEOMICS LA English DT Article ID HUMAN SERUM PROTEOME; 2-DIMENSIONAL ELECTROPHORESIS; HUMAN BLOOD; PROTEINS; DATABASE; BIOINFORMATICS; IDENTIFICATION; SUPPLEMENT; TOOL AB We have merged four different views of the human plasma proteome, based on different methodologies, into a single nonredundant list of 1175 distinct gene products. The methodologies used were 1) literature search for proteins reported to occur in plasma or serum; 2) multidimensional chromatography of proteins followed by two-dimensional electrophoresis and mass spectroscopy ( MS) identification of resolved proteins; 3) tryptic digestion and multidimensional chromatography of peptides followed by MS identification; and 4) tryptic digestion and multidimensional chromatography of peptides from low-molecular-mass plasma components followed by MS identification. Of 1,175 nonredundant gene products, 195 were included in more than one of the four input datasets. Only 46 appeared in all four. Predictions of signal sequence and transmembrane domain occurrence, as well as Genome Ontology annotation assignments, allowed characterization of the nonredundant list and comparison of the data sources. The "nonproteomic" literature ( 468 input proteins) is strongly biased toward signal sequence-containing extracellular proteins, while the three proteomics methods showed a much higher representation of cellular proteins, including nuclear, cytoplasmic, and kinesin complex proteins. Cytokines and protein hormones were almost completely absent from the proteomics data ( presumably due to low abundance), while categories like DNA-binding proteins were almost entirely absent from the literature data ( perhaps unexpected and therefore not sought). Most major categories of proteins in the human proteome are represented in plasma, with the distribution at successively deeper layers shifting from mostly extracellular to a distribution more like the whole ( primarily cellular) proteome. The resulting nonredundant list confirms the presence of a number of interesting candidate marker proteins in plasma and serum. C1 Plasma Proteome Inst, Washington, DC 20009 USA. Large Scale Biol Corp, Proteom Div, Germantown, MD 20876 USA. NCI, Lab Proteom & Analyt Technol, SAIC Frederick Inc, Frederick, MD 21702 USA. Pacific NW Natl Lab, Dept Biol Sci, Richland, WA 99352 USA. Inpharmat Ltd, London W1T 2NU, England. RP Anderson, NL (reprint author), Plasma Proteome Inst, POB 53450, Washington, DC 20009 USA. EM leighanderson@plasmaproteome.org RI Adkins, Joshua/B-9881-2013 OI Adkins, Joshua/0000-0003-0399-0700 NR 22 TC 547 Z9 583 U1 11 U2 62 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 1535-9476 J9 MOL CELL PROTEOMICS JI Mol. Cell. Proteomics PD APR PY 2004 VL 3 IS 4 BP 311 EP 326 DI 10.1074/mcp.M300127-MCP200 PG 16 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 806IF UT WOS:000220426900003 PM 14718574 ER PT J AU De Martino, MU Bhattachryya, N Alesci, S Ichijo, T Chrousos, GP Kino, T AF De Martino, MU Bhattachryya, N Alesci, S Ichijo, T Chrousos, GP Kino, T TI The glucocorticoid receptor and the orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II interact with and mutually affect each other's transcriptional activities: Implications for intermediary metabolism SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE; RETINOIC ACID RECEPTOR; NEGATIVE CROSS-TALK; ACCESSORY FACTORS; COUP-TFII; POSSIBLE MECHANISM; BETA-ISOFORM; DNA-BINDING; PEPCK GENE; EXPRESSION AB Glucocorticoids exert their metabolic effect via their intracellular receptor, the glucocorticoid receptor (GR). In a yeast two-hybrid screening, we found the chicken ovalbumin upstream promoter transcription factor II (COUP-TFII), an orphan nuclear receptor that plays important roles in glucose, cholesterol, and xenobiotic metabolism, as a partner of GR. In an in vitro glutathione-S-transferase pull-down assay, COUP-TFII interacted via its DNA-binding domain with the hinge regions of both GRalpha and its splicing variant GRbeta, whereas COUP-TFII formed a complex with GRalpha, but not with GRbeta, in an in vivo chromatin immunoprecipitation and a regular immunoprecipitation assay. Accordingly, GRalpha, but not GRbeta, enhanced COUP-TFII-induced transactivation of the simple COUP-TFII-responsive 7alpha-hydroxylase promoter through the transcriptional activity of its activation function-1 domain, whereas COUP-TFII repressed GRalpha-induced transactivation of the glucocorticoid-responsive promoter by attracting the silencing mediator for retinoid and thyroid hormone receptors. Importantly, mutual protein-protein interaction of GRalpha and COUP-TFII was necessary for glucocorticoid-induced enhancement of the promoter activity and the endogenous mRNA expression of the COUP-TFII-responsive phosphoenolpyruvate carboxykinase, the rate-limiting enzyme of hepatic gluconeogenesis. We suggest that COUP-TFII may participate in some of the metabolic effects of glucocorticoids through direct interactions with GRalpha. These interactions influence the transcription of both COUP-TFII- and GRalpha-responsive target genes, seem to be promoter specific, and can be in either a positive or negative direction. C1 NICHHD, Pediat & Reprod Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NIDDKD, Growth & Dev Sect, Diabet Branch, NIH, Bethesda, MD 20892 USA. RP Kino, T (reprint author), NICHHD, Pediat & Reprod Endocrinol Branch, NIH, 10 Ctr Dr,Mail Stop Code 1583,Bldg 10,Room 9D42, Bethesda, MD 20892 USA. EM kinot@mail.nih.gov NR 65 TC 30 Z9 30 U1 0 U2 0 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 USA SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD APR 1 PY 2004 VL 18 IS 4 BP 820 EP 833 DI 10.1210/me.2003-0341 PG 14 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 806IG UT WOS:000220427000005 PM 14739255 ER PT J AU Zhang, Y Repa, JJ Inoue, Y Hayhurst, GP Gonzalez, FJ Mangelsdorf, DJ AF Zhang, Y Repa, JJ Inoue, Y Hayhurst, GP Gonzalez, FJ Mangelsdorf, DJ TI Identification of a liver-specific uridine phosphorylase that is regulated by multiple lipid-sensing nuclear receptors SO MOLECULAR ENDOCRINOLOGY LA English DT Article ID BILE-ACID BIOSYNTHESIS; GENE-EXPRESSION; LXR-ALPHA; OXYSTEROL RECEPTORS; LOADED MACROPHAGES; CHOLESTEROL; BINDING; TRANSCRIPTION; METABOLISM; BETA AB In this work, we report the characterization of a novel liver-specific gene (L-UrdPase), whose expression is regulated by a number of hepatic nuclear receptors ( including liver X receptors, peroxisome proliferator-activated receptor alpha, farnesoid X receptor, and hepatic nuclear factor-4alpha), which have been shown to be involved in lipid metabolism. L-UrdPase encodes a previously uncharacterized protein with similarity to an intestine-specific uridine phosphorylase. Enzymatic assays confirmed that L-UrdPase has uridine phosphorylase activity. However, L-UrdPase has a highly restricted, nonoverlapping pattern of expression with its intestinal counterpart and is regulated in a distinct manner by several different nuclear receptors. The identification of the liver uridine phosphorylase and its characterization as a target of lipid-sensing nuclear receptors implies the existence of a previously unknown nuclear receptor signaling pathway that links lipid and uridine metabolism. C1 Univ Texas, SW Med Ctr, Howard Hughes Med Inst, Dallas, TX 75390 USA. Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA. Univ Texas, SW Med Ctr, Touchstone Ctr Diabet Res, Dept Physiol, Dallas, TX 75390 USA. Univ Texas, SW Med Ctr, Touchstone Ctr Diabet Res, Dept Internal Med, Dallas, TX 75390 USA. NCI, Lab Metab, Ctr Canc Res, Bethesda, MD 20892 USA. RP Mangelsdorf, DJ (reprint author), Univ Texas, SW Med Ctr, Howard Hughes Med Inst, 5323 Harry Hines Blvd, Dallas, TX 75390 USA. EM Davo.Mango@utsouthwestern.edu FU NIDDK NIH HHS [1U19 DK 62434] NR 44 TC 17 Z9 18 U1 0 U2 1 PU ENDOCRINE SOC PI BETHESDA PA 4350 EAST WEST HIGHWAY SUITE 500, BETHESDA, MD 20814-4110 USA SN 0888-8809 J9 MOL ENDOCRINOL JI Mol. Endocrinol. PD APR 1 PY 2004 VL 18 IS 4 BP 851 EP 862 DI 10.1210/me.2003-0285 PG 12 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 806IG UT WOS:000220427000007 PM 14715930 ER PT J AU Preusch, PC AF Preusch, PC TI Integrative and organ systems pharmacology: A new initiative from the national institute of general medical sciences SO MOLECULAR INTERVENTIONS LA English DT Review C1 NIGMS, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Preusch, PC (reprint author), NIGMS, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. EM preuschp@nigms.nih.gov NR 6 TC 11 Z9 11 U1 0 U2 1 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 1534-0384 J9 MOL INTERV JI Mol. Interv. PD APR PY 2004 VL 4 IS 2 BP 72 EP 73 DI 10.1124/mi.4.2.1 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 832DX UT WOS:000222248500001 PM 15087477 ER PT J AU Murphy, DL Lerner, A Rudnick, G Lesch, KP AF Murphy, DL Lerner, A Rudnick, G Lesch, KP TI Serotonin transporter: Gene, genetic disorders, and pharmacogenetics SO MOLECULAR INTERVENTIONS LA English DT Review ID OBSESSIVE-COMPULSIVE DISORDER; POSITRON-EMISSION-TOMOGRAPHY; HUMAN NOREPINEPHRINE TRANSPORTER; PRIMARY PULMONARY-HYPERTENSION/; DEFICIT HYPERACTIVITY DISORDER; ANTIDEPRESSANT-INDUCED MANIA; POLYMORPHIC REGION 5-HTTLPR; BIOGENIC-AMINE TRANSPORTERS; IRRITABLE-BOWEL-SYNDROME; CENTRAL-NERVOUS-SYSTEM AB The highly evolutionarily conserved serotonin transporter (SERT) regulates the entire serotoninergic system and its receptors via modulation of extracellular fluid serotonin concentrations. Differences in SERT expression and function produced by three SERT genes and their variants show associations with multiple human disorders. Screens of DNA from patients with autism, ADHD, bipolar disorder, and Tourette's syndrome have detected signals in the chromosome 17q region where SERT is located. Parallel investigations of SERT knockout mice have uncovered multiple phenotypes that identify SERT as a candidate gene for additional human disorders ranging from irritable bowel syndrome to obesity. Replicated studies have demonstrated that the SERT 5'-flanking region polymorphism SS genotype is associated with poorer therapeutic responses and more frequent serious side effects during treatment with antidepressant SERT antagonists, namely, the serotonin reuptake inhibitors (SRIs). C1 NIMH, Clin Sci Lab, NIH, Bethesda, MD 20892 USA. Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA. Univ Wurzburg, Dept Psychiat & Psychotherapy, D-97070 Wurzburg, Germany. RP Murphy, DL (reprint author), NIMH, Clin Sci Lab, NIH, Bldg 10,Room 3D41,10 Ctr Dr,MSC 1264, Bethesda, MD 20892 USA. EM murphyd@intra.nimh.nih.gov RI Lesch, Klaus-Peter/J-4906-2013 OI Lesch, Klaus-Peter/0000-0001-8348-153X NR 158 TC 229 Z9 251 U1 5 U2 31 PU AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3995 USA SN 1534-0384 J9 MOL INTERV JI Mol. Interv. PD APR PY 2004 VL 4 IS 2 BP 109 EP 123 DI 10.1124/mi.4.2.8 PG 15 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 832DX UT WOS:000222248500008 PM 15087484 ER PT J AU Senatorov, VV Ren, M Kanai, H Wei, H Chuang, DM AF Senatorov, VV Ren, M Kanai, H Wei, H Chuang, DM TI Short-term lithium treatment promotes neuronal survival and proliferation in rat striatum infused with quinolinic acid, an excitotoxic model of Huntington's disease SO MOLECULAR PSYCHIATRY LA English DT Article DE lithium; striatum; quinolinic acid; apoptosis; proliferation; Bcl-2 ID RECEPTOR-MEDIATED EXCITOTOXICITY; CEREBRAL CORTICAL-NEURONS; SYNTHASE KINASE 3-BETA; NITRIC-OXIDE SYNTHASE; NEUROTROPHIC FACTOR; GLUTAMATE EXCITOTOXICITY; TREATMENT INCREASES; NADPH-DIAPHORASE; NERVOUS-SYSTEM; ANIMAL-MODELS AB We assessed the ability of lithium to reduce neurodegeneration and to stimulate cell proliferation in a rat model of Huntington's disease in which quinolinic acid (QA) was unilaterally infused into the striatum. LiCl (0.5-3.0mEq/kg) was injected subcutaneously 24 h before and 1 h after QA infusion. At 7 days after QA injection, lithium significantly diminished the loss of neurons immunostained for Neuronal Nuclei (NeuN) in the injured striatum, but failed to prevent the reduction of NADPH-diaphorase-positive striatal interneurons. Lithium also reduced the number of neurons showing DNA damage or activated caspase-3. This neuroprotection was associated with an upregulation of Bcl-2 protein levels in the striatal tissue and an increase in the number and density of Bcl-2 immunostaining in striatal neurons. Bromodeoxyuridinie (BrdU) labeling in the lithium-treated injured striatum revealed the presence of large numbers of proliferating cells near the QA-injection site, with a reduction of BrdU-labeled cells in the subventricular zone (SVZ). All BrdU-labeled cells in the SVZ and the majority of BrdU-labeled cells near the QA-injection site were negative for either NeuN or glial fibrillary acidic protein (GFAP), suggesting that they are undifferentiated progenitor cells. However, a small number of BrdU-positive cells found in the QA-injected and lithium-treated striatum site were positive for either NeuN or GFAP. Our results suggest that lithium is neuroprotective in the QA-injection model of Huntington's disease not only due to its ability to inhibit apoptosis but also because it can stimulate neuronal and astroglial progenitor proliferation in the QA-injected striatum or their migration from the SVZ. C1 NIMH, Mol Neurobiol Sect, NIH, Bethesda, MD 20892 USA. RP Chuang, DM (reprint author), NIMH, Mol Neurobiol Sect, NIH, Bldg 10,Room 4C206,10 Ctr Dr,MSC 1363, Bethesda, MD 20892 USA. EM chuang@mail.nih.gov NR 50 TC 64 Z9 74 U1 1 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1359-4184 J9 MOL PSYCHIATR JI Mol. Psychiatr. PD APR PY 2004 VL 9 IS 4 BP 371 EP 385 DI 10.1038/sj.mp.4001463 PG 15 WC Biochemistry & Molecular Biology; Neurosciences; Psychiatry SC Biochemistry & Molecular Biology; Neurosciences & Neurology; Psychiatry GA 806QQ UT WOS:000220448800003 PM 14702090 ER PT J AU Song, L Chau, L Sakamoto, Y Nakashima, J Koide, M Tuan, RS AF Song, L Chau, L Sakamoto, Y Nakashima, J Koide, M Tuan, RS TI Electric field-induced molecular vibration for noninvasive, high-efficiency DNA transfection SO MOLECULAR THERAPY LA English DT Article DE gene delivery; DNA transfection; mesenchymal stem cells; sternum; muscle; osteogenesis; adipogenesis; chondrogenesis ID MESENCHYMAL STEM-CELLS; MEDIATED GENE-TRANSFER; MAMMALIAN-CELLS; IN-VITRO; PROGENITOR CELLS; CULTURED-CELLS; WIDE-RANGE; BONE; PERMEABILIZATION; ELECTROPORATION AB Gene delivery is an essential research tool for elucidating gene structure, regulation, and function in biomedical research and is the technological basis for gene therapy. However, the application of nonviral vectors in mammalian cell transfection and gene therapy is limited in that current methods require large amounts of exogenous DNA and/or exhibit high cytotoxicity and low transfection efficiency in primary cells. Here we describe the development of a novel, noninvasive gene delivery protocol using plasmid DNA vectors, based on the principle of electric field-induced molecular vibration. This method enables foreign DNA molecules to penetrate the plasma membrane and enter the cytoplasm of both primary mesenchymal progenitor cells and established cell lines of various species, at high efficiency and with low cell mortality. This procedure requires no special reagents, allows stable expression of transduced DNA, and does not interfere with the normal cellular differentiation activities of human and chick mesenchymal progenitors. C1 NIAMSD, Cartilage Biol & Orthopaed Branch, Dept Hlth & Human Sci, NIH, Bethesda, MD 20892 USA. Mollennium Labs Inc, Nisshin, Aichi 4700126, Japan. RP Tuan, RS (reprint author), NIAMSD, Cartilage Biol & Orthopaed Branch, Dept Hlth & Human Sci, NIH, Bldg 50,Room 1503,MSC8022, Bethesda, MD 20892 USA. EM Tuanr@mail.nih.gov FU NIAMS NIH HHS [Z01AR41131] NR 40 TC 31 Z9 34 U1 0 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1525-0016 J9 MOL THER JI Mol. Ther. PD APR PY 2004 VL 9 IS 4 BP 607 EP 616 DI 10.1016/j.ymthe.2004.01.017 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA 812AI UT WOS:000220812000018 PM 15093191 ER PT J AU Singer, HS Loiselle, CR Lee, O Minzer, K Swedo, S Grus, FH AF Singer, HS Loiselle, CR Lee, O Minzer, K Swedo, S Grus, FH TI Anti-basal ganglia antibodies in PANDAS SO MOVEMENT DISORDERS LA English DT Article DE PANDAS; anti-basal ganglia antibodies; ELISA; Western blot; discriminant analysis ID OBSESSIVE-COMPULSIVE DISORDER; REPERTOIRES WESTERN BLOTS; TOURETTES-SYNDROME; NEUROPSYCHIATRIC DISORDERS; STREPTOCOCCAL INFECTIONS; ANTINEURONAL ANTIBODIES; NATURAL AUTOANTIBODIES; DISCRIMINANT-ANALYSIS; STRIATAL ANTIBODIES; NEUROBLASTOMA CELL AB An autoimmune-mediated mechanism involving molecular mimicry has been proposed for a variety of pediatric movement disorders that occur after a streptococcal infection. In this study, anti-basal ganglia antibodies (ABGA) were measured in 15 children with the diagnosis of pediatric autoimmune neuropsychiatric disorder associated with streptococcal infection (PANDAS) and compared with those in 15 controls. ELISA and Western immunoblotting (WB) methods were used to detect ABGA against supernatant (S1), pellet (P2), and synaptosomal preparations from adult postmortem caudate, putamen, and globus pallidus. ELISA optical density values did not differ between PANDAS patients and controls across all preparations. Immunoblotting identified multiple bands in all subjects with no differences in the number of bands or their total density. Discriminant analysis, used to assess mean binding patterns, showed that PANDAS patients differed from controls only for the caudate S1 fraction (Wilks' lambda = 0.0236, P < 0.0002), with PANDAS-primarily tic subjects providing the greatest discrimination. Among the epitopes contributing to differences between PANDAS and control in the caudate S1 fraction, mean binding to the epitope at 183 kDa was the most different between groups. In conclusion, ELISA measurements do not differentiate between PANDAS and controls, suggesting a lack of major antibody changes in this disorder. Further immunoblot analyses using a caudate supernatant fraction are required to completely exclude the possibility of minor antibody repertoire differences in PANDAS subjects, especially in those who primarily have tics. (C) 2004 Movement Disorder Society. C1 Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA. Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA. NIMH, Pediat & Dev Neuropsychiat Branch, Bethesda, MD 20892 USA. Univ Mainz, Augenklin, Dept Ophthalmol, D-6500 Mainz, Germany. RP Singer, HS (reprint author), Johns Hopkins Univ Hosp, Div Pediat Neurol, Jefferson St Bldg 124,600 N Wolfe St, Baltimore, MD 21287 USA. EM hsinger@jhmi.edu FU NINDS NIH HHS [R01 NS37706] NR 43 TC 66 Z9 73 U1 3 U2 4 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0885-3185 J9 MOVEMENT DISORD JI Mov. Disord. PD APR PY 2004 VL 19 IS 4 BP 406 EP 415 DI 10.1002/mds.20052 PG 10 WC Clinical Neurology SC Neurosciences & Neurology GA 812OO UT WOS:000220849000006 PM 15077238 ER PT J AU Gilbert, DL Bansal, AS Sethuraman, G Sallee, FR Zhang, J Lipps, T Wassermann, EM AF Gilbert, DL Bansal, AS Sethuraman, G Sallee, FR Zhang, J Lipps, T Wassermann, EM TI Association of cortical disinhibition with tic, ADHD, and OCD severity in Tourette syndrome SO MOVEMENT DISORDERS LA English DT Article DE Tourette syndrome; ADHD; cortical inhibition; transcranial magnetic stimulation; children; adults ID TRANSCRANIAL MAGNETIC STIMULATION; DEFICIT-HYPERACTIVITY DISORDER; HUMAN MOTOR CORTEX; BASAL GANGLIA; INHIBITION; CHILDREN; EXCITABILITY; ORGANIZATION; PROJECTIONS; EXCITATION AB Hyperkinetic disorders may involve excess excitatory output from thalamus to cerebral cortex. Case-control, neurophysiological studies in persons with Tourette Syndrome (TS), Attention Deficit Hyperactivity Disorder (ADHD), and Obsessive-Compulsive Disorder (OCD) support this model. To compare the strength of association between motor cortex inhibition and tic, ADHD, and OCD severity in TS, we used transcranial magnetic stimulation to measure motor cortex inhibition in 36 children and adults with TS. Current symptom severity was assessed with standard clinical rating scales and compared with neurophysiological measures using correlational and multivariate regression analyses. Severity of ADHD symptoms and motor tics were associated significantly and independently with short interval intracortical inhibition (SICI) (r(2) = 0.50; F[2,27] 13.7; P < 0.001), particularly in subjects not taking neuroleptics (r(2) = 0.68; F[2,17] = 17.8; P < 0.0001). The correlation of cortical disinhibition was greater with ADHD symptoms severity (r = 0.53; P = 0.003) than with tic severity (r = 0.42; P = 0.02), suggesting that in TS, the association between SICI and ADHD symptoms may be more consistent or direct than the association between SICI and tics. (C) 2004 Movement Disorder Society. C1 Cincinnati Childrens Hosp, Med Ctr, Div Neurol, Cincinnati, OH 45229 USA. NE Ohio Univ, Coll Med, Rootstown, OH 44272 USA. Univ Cincinnati, Div Psychiat, Cincinnati, OH USA. NINDS, Brain Stimulat Unit, Bethesda, MD 20892 USA. RP Gilbert, DL (reprint author), Cincinnati Childrens Hosp, Med Ctr, Div Neurol, ML 2015,3333 Burnet Ave, Cincinnati, OH 45229 USA. EM d.gilbert@cchmc.org RI Gilbert, Donald/D-6443-2016 OI Gilbert, Donald/0000-0002-9245-6878 FU NINDS NIH HHS [K23 NS41920] NR 55 TC 82 Z9 84 U1 1 U2 4 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0885-3185 J9 MOVEMENT DISORD JI Mov. Disord. PD APR PY 2004 VL 19 IS 4 BP 416 EP 425 DI 10.1002/mds.20044 PG 10 WC Clinical Neurology SC Neurosciences & Neurology GA 812OO UT WOS:000220849000007 PM 15077239 ER PT J AU Gibbs, RA Weinstock, GM Metzker, ML Muzny, DM Sodergren, EJ Scherer, S Scott, G Steffen, D Worley, KC Burch, PE Okwuonu, G Hines, S Lewis, L DeRamo, C Delgado, O Dugan-Rocha, S Miner, G Morgan, M Hawes, A Gill, R Holt, RA Adams, MD Amanatides, PG Baden-Tillson, H Barnstead, M Chin, S Evans, CA Ferriera, S Fosler, C Glodek, A Gu, ZP Jennings, D Kraft, CL Nguyen, T Pfannkoch, CM Sitter, C Sutton, GG Venter, JC Woodage, T Smith, D Lee, HM Gustafson, E Cahill, P Kana, A Doucette-Stamm, L Weinstock, K Fechtel, K Weiss, RB Dunn, DM Green, ED Blakesley, RW Bouffard, GG de Jong, J Osoegawa, K Zhu, BL Marra, M Schein, J Bosdet, I Fjell, C Jones, S Krzywinski, M Mathewson, C Siddiqui, A Wye, N McPherson, J Zhao, SY Fraser, CM Shetty, J Shatsman, S Geer, K Chen, YX Abramzon, S Nierman, WC Gibbs, RA Weinstock, GM Havlak, PH Chen, R Durbin, KJ Egan, A Ren, YR Song, XZ Li, BS Liu, Y Qin, X Cawley, S Weinstock, GM Worley, KC Cooney, AJ Gibbs, RA D'Souza, LM Martin, K Wu, JQ Gonzalez-Garay, ML Jackson, AR Kalafus, KJ McLeod, MP Milosavljevic, A Virk, D Volkov, A Wheeler, DA Zhang, ZD Bailey, JA Eichler, EE Tuzun, E Birney, E Mongin, E Ureta-Vidal, A Woodwark, C Zdobnov, E Bork, P Suyama, M Torrents, D Alexandersson, M Trask, BJ Young, JM Smith, D Huang, H Fechtel, K Wang, HJ Xing, HM Weinstock, K Daniels, S Gietzen, D Schmidt, J Stevens, K Vitt, U Wingrove, J Camara, F Schmidt, J Stevens, K Vitt, U Wingrove, J Camara, F Alba, MM Abril, JF Guigo, R Smit, A Dubchak, I Rubin, EM Couronne, O Poliakov, A Hubner, N Ganten, D Goesele, C Hummel, O Kreitler, T Lee, YA Monti, J Schulz, H Zimdahl, H Himmelbauer, H Lehrach, H Jacob, HJ Bromberg, S Gullings-Handley, J Jensen-Seaman, MI Kwitek, AE Lazar, J Pasko, D Tonellato, PJ Twigger, S Ponting, P Duarte, JM Rice, S Goodstadt, L Beatson, SA Emes, RD Winter, EE Webber, C Brandt, P Nyakatura, G Adetobi, M Chiaromonte, F Elnitski, L Eswara, P Hardison, RC Hou, MM Kolbe, D Makova, K Miller, W Nekrutenko, A Riemer, C Schwartz, S Taylor, J Yang, S Zhang, Y Lindpaintner, K Andrews, TD Caccamo, M Clamp, M Clarke, L Curwen, V Durbin, R Eyras, E Searle, SM Cooper, GM Batzoglou, S Brudno, M Sidow, A Stone, EA Venter, JC Payseur, BA Bourque, G Lopez-Otin, C Puente, XS Chakrabarti, K Chatterji, S Dewey, C Pachter, L Bray, N Yap, VB Caspi, A Tesler, G Pevzner, PA Haussler, D Roskin, KM Baertsch, R Clawson, H Furey, TS Hinrichs, AS Karolchik, D Kent, WJ Rosenbloom, KR Trumbower, H Weirauch, M Cooper, DN Stenson, PD Ma, B Brent, M Arumugam, M Shteynberg, D Copley, RR Taylor, MS Riethman, H Mudunuri, U Peterson, J Guyer, M Felsenfeld, A Old, S Mockrin, S Collins, F AF Gibbs, RA Weinstock, GM Metzker, ML Muzny, DM Sodergren, EJ Scherer, S Scott, G Steffen, D Worley, KC Burch, PE Okwuonu, G Hines, S Lewis, L DeRamo, C Delgado, O Dugan-Rocha, S Miner, G Morgan, M Hawes, A Gill, R Holt, RA Adams, MD Amanatides, PG Baden-Tillson, H Barnstead, M Chin, S Evans, CA Ferriera, S Fosler, C Glodek, A Gu, ZP Jennings, D Kraft, CL Nguyen, T Pfannkoch, CM Sitter, C Sutton, GG Venter, JC Woodage, T Smith, D Lee, HM Gustafson, E Cahill, P Kana, A Doucette-Stamm, L Weinstock, K Fechtel, K Weiss, RB Dunn, DM Green, ED Blakesley, RW Bouffard, GG de Jong, J Osoegawa, K Zhu, BL Marra, M Schein, J Bosdet, I Fjell, C Jones, S Krzywinski, M Mathewson, C Siddiqui, A Wye, N McPherson, J Zhao, SY Fraser, CM Shetty, J Shatsman, S Geer, K Chen, YX Abramzon, S Nierman, WC Gibbs, RA Weinstock, GM Havlak, PH Chen, R Durbin, KJ Egan, A Ren, YR Song, XZ Li, BS Liu, Y Qin, X Cawley, S Weinstock, GM Worley, KC Cooney, AJ Gibbs, RA D'Souza, LM Martin, K Wu, JQ Gonzalez-Garay, ML Jackson, AR Kalafus, KJ McLeod, MP Milosavljevic, A Virk, D Volkov, A Wheeler, DA Zhang, ZD Bailey, JA Eichler, EE Tuzun, E Birney, E Mongin, E Ureta-Vidal, A Woodwark, C Zdobnov, E Bork, P Suyama, M Torrents, D Alexandersson, M Trask, BJ Young, JM Smith, D Huang, H Fechtel, K Wang, HJ Xing, HM Weinstock, K Daniels, S Gietzen, D Schmidt, J Stevens, K Vitt, U Wingrove, J Camara, F Schmidt, J Stevens, K Vitt, U Wingrove, J Camara, F Alba, MM Abril, JF Guigo, R Smit, A Dubchak, I Rubin, EM Couronne, O Poliakov, A Hubner, N Ganten, D Goesele, C Hummel, O Kreitler, T Lee, YA Monti, J Schulz, H Zimdahl, H Himmelbauer, H Lehrach, H Jacob, HJ Bromberg, S Gullings-Handley, J Jensen-Seaman, MI Kwitek, AE Lazar, J Pasko, D Tonellato, PJ Twigger, S Ponting, P Duarte, JM Rice, S Goodstadt, L Beatson, SA Emes, RD Winter, EE Webber, C Brandt, P Nyakatura, G Adetobi, M Chiaromonte, F Elnitski, L Eswara, P Hardison, RC Hou, MM Kolbe, D Makova, K Miller, W Nekrutenko, A Riemer, C Schwartz, S Taylor, J Yang, S Zhang, Y Lindpaintner, K Andrews, TD Caccamo, M Clamp, M Clarke, L Curwen, V Durbin, R Eyras, E Searle, SM Cooper, GM Batzoglou, S Brudno, M Sidow, A Stone, EA Venter, JC Payseur, BA Bourque, G Lopez-Otin, C Puente, XS Chakrabarti, K Chatterji, S Dewey, C Pachter, L Bray, N Yap, VB Caspi, A Tesler, G Pevzner, PA Haussler, D Roskin, KM Baertsch, R Clawson, H Furey, TS Hinrichs, AS Karolchik, D Kent, WJ Rosenbloom, KR Trumbower, H Weirauch, M Cooper, DN Stenson, PD Ma, B Brent, M Arumugam, M Shteynberg, D Copley, RR Taylor, MS Riethman, H Mudunuri, U Peterson, J Guyer, M Felsenfeld, A Old, S Mockrin, S Collins, F CA Rat Genome Sequencing Project Cons TI Genome sequence of the Brown Norway rat yields insights into mammalian evolution SO NATURE LA English DT Review ID ARTIFICIAL CHROMOSOME LIBRARY; RECENT SEGMENTAL DUPLICATIONS; MURINE ENDOGENOUS RETROVIRUS; EMBRYONIC EPSILON-GLOBIN; MAJOR URINARY PROTEINS; LOCUS-CONTROL REGION; MOUSE GENOME; DNA-SEQUENCE; GENE PREDICTION; ANIMAL-MODEL AB The laboratory rat (Rattus norvegicus) is an indispensable tool in experimental medicine and drug development, having made inestimable contributions to human health. We report here the genome sequence of the Brown Norway (BN) rat strain. The sequence represents a high-quality 'draft' covering over 90% of the genome. The BN rat sequence is the third complete mammalian genome to be deciphered, and three-way comparisons with the human and mouse genomes resolve details of mammalian evolution. This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineage-independent evolutionary events such as expansion of gene families, orthology relations and protein evolution. C1 Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, Houston, TX 77030 USA. British Columbia Canc Agcy, Canadas Michael Smith Genome Sci Ctr, Vancouver, BC V5Z 4E6, Canada. Celera, Rockville, MD 20850 USA. Case Western Reserve Univ, Sch Med, Dept Genet, Cleveland, OH 44106 USA. Case Western Reserve Univ, Sch Med, Ctr Computat Gen, Cleveland, OH 44106 USA. DSM Pharmaceut Inc, Greenville, NC 27834 USA. Inst Biol Energy Alternat, Rockville, MD 20850 USA. Intronn Inc, Gaithersburg, MD 20878 USA. Ctr Advancement Gen TCAG, Rockville, MD 20850 USA. Avalon Pharmaceut, Germantown, MD 20876 USA. NIAID, Basic Immunol Branch, Div Allergy Immunol & Transplantat, NIH,DHHS, Bethesda, MD 20892 USA. DynPort Vaccine Co LLC, Frederick, MD 21702 USA. Genome Therapeut Corp, Waltham, MA 02453 USA. Agencourt Biosci Corp, Beverly, MA 01915 USA. Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA. NHGRI, NIH, Intramural Sequencing Ctr, NISC, Bethesda, MD 20892 USA. NHGRI, Genome Technol Branch, NIH, Bethesda, MD 20892 USA. Childrens Hosp, Oakland Res Inst, BACPAC Resources, Oakland, CA 94609 USA. Washington Univ, Sch Med, Genome Sequencing Ctr, St Louis, MO 63108 USA. Inst Gen Res, Rockville, MD 20850 USA. Affymetrix, Oakland, CA 94608 USA. Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA. EBI, Hinxton CB10 1SD, Cambs, England. European Mol Biol Lab, D-69117 Heidelberg, Germany. Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany. Fraunhofer Chalmers Res Ctr Ind Math, S-41288 Gothenburg, Sweden. Fred Hutchinson Canc Res Ctr, Div Human Biol, Seattle, WA 98109 USA. Incyte Corp, Palo Alto, CA 94304 USA. Univ Pompeu Fabra, Inst Municipal Invest Med, Grp Rec Informat Biomedia, Barcelona 08003, Spain. Ctr Regulacio Gen, Programa Bioinformat & Gen, Barcelona 08003, Spain. Inst Syst Biol, Computat Biol Grp, Seattle, WA 98103 USA. Univ Calif Berkeley, Lawrence Berkeley Lab, Gen Div, Berkeley, CA 94720 USA. US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. Max Planck Inst Mol Genet, D-14195 Berlin, Germany. Med Coll Wisconsin, Human & Mol Genet Ctr, Bioinformat Res Ctr, Milwaukee, WI 53226 USA. Med Coll Wisconsin, Dept Physiol, Milwaukee, WI 53226 USA. Med Coll Wisconsin, Bioinformat Res Ctr, Rat Genome Database, Milwaukee, WI 53226 USA. Univ Oxford, MRC, Funct Genet Unit, Dept Human Anat & Genet, Oxford OX1 3QX, England. MWG Biotech, D-85560 Ebersberg, Germany. Penn State Univ, Dept Biol, Huck Inst Life Sci, Ctr Comparat Gen & Bioinformat, University Pk, PA 16802 USA. Penn State Univ, Dept Stat, University Pk, PA 16802 USA. Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. Penn State Univ, Dept Comp Sci & Engn, University Pk, PA 16802 USA. Penn State Univ, Dept Hlth Evaluat Sci, University Pk, PA 16802 USA. F Hoffmann La Roche Ltd, Roche Genet & Roche Ctr Med Gen, CH-4070 Basel, Switzerland. Sanger Inst, Hinxton CB10 1SA, Cambs, England. Stanford Univ, Dept Pathol, Stanford, CA 94305 USA. Stanford Univ, Dept Genet, Stanford, CA 94305 USA. Stanford Univ, Dept Comp Sci, James H Clark Ctr S256, Stanford, CA 94305 USA. Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ 85721 USA. Univ Montreal, Ctr Rech Math, Montreal, PQ H3T 1J8, Canada. Univ Oviedo, Inst Univ Oncol, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain. Univ Calif Berkeley, Dept Elect Engn & Comp Sci, Berkeley, CA 94720 USA. Univ Calif Berkeley, Dept Math, Berkeley, CA 94720 USA. Univ Calif Berkeley, Bioengn Grad Grp, Berkeley, CA 94720 USA. Univ Calif San Diego, Dept Math, La Jolla, CA 92093 USA. Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 USA. Univ Calif Santa Cruz, Baskin Sch Engn, Ctr Biomol Sci & Engn, Howard Hughes Med Inst, Santa Cruz, CA 95064 USA. Univ Calif Santa Cruz, Baskin Sch Engn, Ctr Biomol Sci & Engn, Genome Bioinformat Grp, Santa Cruz, CA 95064 USA. Cardiff Univ, Inst Med Genet, Cardiff CF14 4XN, S Glam, Wales. Univ Western Ontario, Dept Comp Sci, London, ON N6A 5B7, Canada. Washington Univ, Lab Computat Gen, St Louis, MO 63130 USA. Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 7BN, England. Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA. NHGRI, US NIH, Bethesda, MD 20892 USA. NHLBI, US NIH, Bethesda, MD 20892 USA. Baylor Coll Med, Program Struct & Computat Biol & Mol Biophys, Houston, TX 77030 USA. RP Gibbs, RA (reprint author), Baylor Coll Med, Dept Mol & Human Genet, Human Genome Sequencing Ctr, MS BCM226,1 Baylor Plaza, Houston, TX 77030 USA. EM agibbs@bcm.tmc.edu RI Torrents, David/G-5785-2015; Bork, Peer/F-1813-2013; Abril Ferrando, Josep/B-3877-2014; Tang, Macy/B-9798-2014; Eyras, Eduardo/L-1053-2014; Camara Ferreira, Francisco/G-9841-2015; Guigo, Roderic/D-1303-2010; Marra, Marco/B-5987-2008; Beatson, Scott/B-6985-2013; Jones, Steven/C-3621-2009; Lopez-Otin, Carlos/C-6657-2013; Stone, Eric/Q-7840-2016; Gasull, Martina/A-6630-2013; Taylor, James/F-1026-2011; Alba, Mar/B-4793-2009; Li, Bingshan/H-1944-2011; Emes, Richard/C-5341-2008; Couronne, Olivier Couronne/G-1244-2012; Holt, Robert/C-3303-2009; Zdobnov, Evgeny/K-1133-2012; Ma, Bin/C-7550-2013; Taylor, Martin/C-3825-2009; Hardison, Ross/G-1142-2010; Eyras, Eduardo/A-1560-2010; Cooper, Gregory/D-6914-2011; Arumugam, Manimozhiyan/E-1211-2011; Cooper, David/H-4384-2011 OI Tuzun, Eray/0000-0002-5550-7816; Nekrutenko, Anton/0000-0002-5987-8032; Delgado, Oliver/0000-0003-2547-5857; Clarke, Laura/0000-0002-5989-6898; Birney, Ewan/0000-0001-8314-8497; Hinrichs, Angie/0000-0002-1697-1130; Ponting, Chris/0000-0003-0202-7816; Andrews, Dan/0000-0003-3922-6376; Furey, Terry/0000-0001-5546-9672; Durbin, Richard/0000-0002-9130-1006; Duarte, Jose Manuel/0000-0002-9544-5621; Torrents, David/0000-0002-6086-9037; Fraser, Claire/0000-0003-1462-2428; Suarez-Puente, Xose/0000-0001-9525-1483; Bork, Peer/0000-0002-2627-833X; Abril Ferrando, Josep/0000-0001-7793-589X; Eyras, Eduardo/0000-0003-0793-6218; Camara Ferreira, Francisco/0000-0002-1971-5466; Guigo, Roderic/0000-0002-5738-4477; Beatson, Scott/0000-0002-1806-3283; Lopez-Otin, Carlos/0000-0001-6964-1904; Taylor, James/0000-0001-5079-840X; Alba, Mar/0000-0002-7963-7375; Emes, Richard/0000-0001-6855-5481; Taylor, Martin/0000-0001-7656-330X; Hardison, Ross/0000-0003-4084-7516; Cooper, Gregory/0000-0001-5509-9923; Arumugam, Manimozhiyan/0000-0002-0886-9101; Cooper, David/0000-0002-8943-8484 FU NHGRI NIH HHS [U01 HG002137, R01 HG002939, U01 HG002137-02S2, U54 HG003273]; NHLBI NIH HHS [R01 HL064541] NR 226 TC 1237 Z9 1993 U1 12 U2 144 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 J9 NATURE JI Nature PD APR 1 PY 2004 VL 428 IS 6982 BP 493 EP 521 DI 10.1038/nature02426 PG 29 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 807ZT UT WOS:000220540100032 PM 15057822 ER PT J AU Grimwood, J Gordon, LA Olsen, A Terry, A Schmutz, J Lamerdin, J Hellsten, U Goodstein, D Couronne, O Gyamfi, MT Aerts, A Altherr, M Ashworth, L Bajorek, E Black, S Branscomb, E Caenepeel, S Carrano, A Caoile, C Chan, YM Christensen, M Cleland, CA Copeland, A Dalin, E Dehal, P Denys, M Detter, JC Escobar, J Flowers, D Fotopulos, D Garcia, C Georgescu, AM Glavina, T Gomez, M Gonzales, E Groza, M Hammon, N Hawkins, T Haydu, L Ho, I Huang, W Israni, S Jett, J Kadner, K Kimball, H Kobayashi, A Larionov, V Leem, SH Lopez, F Lou, YL Lowry, S Malfatti, S Martinez, D McCready, P Medina, C Morgan, J Nelson, K Nolan, M Ovcharenko, I Pitluck, S Pollard, M Popkie, AP Predki, P Quan, G Ramirez, L Rash, S Retterer, J Rodriguez, A Rogers, S Salamov, A Salazar, A She, XW Smith, D Slezak, T Solovyev, V Thayer, N Tice, H Tsai, M Ustaszewska, A Vo, N Wagner, M Wheeler, J Wu, K Xie, G Yang, J Dubchak, I Furey, TS DeJong, P Dickson, M Gordon, D Eichler, EE Pennacchio, LA Richardson, P Stubbs, L Rokhsar, DS Myers, RM Rubin, EM Lucas, SM AF Grimwood, J Gordon, LA Olsen, A Terry, A Schmutz, J Lamerdin, J Hellsten, U Goodstein, D Couronne, O Gyamfi, MT Aerts, A Altherr, M Ashworth, L Bajorek, E Black, S Branscomb, E Caenepeel, S Carrano, A Caoile, C Chan, YM Christensen, M Cleland, CA Copeland, A Dalin, E Dehal, P Denys, M Detter, JC Escobar, J Flowers, D Fotopulos, D Garcia, C Georgescu, AM Glavina, T Gomez, M Gonzales, E Groza, M Hammon, N Hawkins, T Haydu, L Ho, I Huang, W Israni, S Jett, J Kadner, K Kimball, H Kobayashi, A Larionov, V Leem, SH Lopez, F Lou, YL Lowry, S Malfatti, S Martinez, D McCready, P Medina, C Morgan, J Nelson, K Nolan, M Ovcharenko, I Pitluck, S Pollard, M Popkie, AP Predki, P Quan, G Ramirez, L Rash, S Retterer, J Rodriguez, A Rogers, S Salamov, A Salazar, A She, XW Smith, D Slezak, T Solovyev, V Thayer, N Tice, H Tsai, M Ustaszewska, A Vo, N Wagner, M Wheeler, J Wu, K Xie, G Yang, J Dubchak, I Furey, TS DeJong, P Dickson, M Gordon, D Eichler, EE Pennacchio, LA Richardson, P Stubbs, L Rokhsar, DS Myers, RM Rubin, EM Lucas, SM TI The DNA sequence and biology of human chromosome 19 SO NATURE LA English DT Article ID SITU HYBRIDIZATION MAP; HUMAN-GENOME-PROJECT; SEGMENTAL DUPLICATIONS; THYMINE DIMERS; GENE-CLUSTER; LDL RECEPTOR; MOUSE; EVOLUTION; RECOMBINATION; ORGANIZATION AB Chromosome 19 has the highest gene density of all human chromosomes, more than double the genome-wide average. The large clustered gene families, corresponding high G+C content, CpG islands and density of repetitive DNA indicate a chromosome rich in biological and evolutionary significance. Here we describe 55.8 million base pairs of highly accurate finished sequence representing 99.9% of the euchromatin portion of the chromosome. Manual curation of gene loci reveals 1,461 protein-coding genes and 321 pseudogenes. Among these are genes directly implicated in mendelian disorders, including familial hypercholesterolaemia and insulin-resistant diabetes. Nearly one-quarter of these genes belong to tandemly arranged families, encompassing more than 25% of the chromosome. Comparative analyses show a fascinating picture of conservation and divergence, revealing large blocks of gene orthology with rodents, scattered regions with more recent gene family expansions and deletions, and segments of coding and non-coding conservation with the distant fish species Takifugu. C1 Stanford Univ, Sch Med, Dept Genet, Stanford Human Genome Ctr, Palo Alto, CA 94304 USA. DOEs Joint Genome Inst, Walnut Creek, CA 94598 USA. Lawrence Livermore Natl Lab, Livermore, CA 94550 USA. Los Alamos Natl Lab, Los Alamos, NM 87545 USA. NCI, Lab Biosyst & Canc, NIH, Bethesda, MD 20892 USA. Case Western Reserve Univ, Sch Med, Ctr Computat Genom, Dept Genet, Cleveland, OH 44106 USA. Case Western Reserve Univ, Sch Med, Ctr Human Genet, Cleveland, OH 44106 USA. Univ Hosp Cleveland, Cleveland, OH 44106 USA. Univ Calif Santa Cruz, Ctr Biomol Sci & Engn, Santa Cruz, CA 95064 USA. Childrens Hosp Oakland, Oakland, CA 94609 USA. Univ Washington, Howard Hughes Med Inst, Dept Genome Sci, Seattle, WA 98195 USA. Univ Washington, Seattle, WA 98195 USA. RP Grimwood, J (reprint author), Stanford Univ, Sch Med, Dept Genet, Stanford Human Genome Ctr, 975 Calif Ave, Palo Alto, CA 94304 USA. EM jane@shgc.stanford.edu; emrubin@lbl.gov RI Couronne, Olivier Couronne/G-1244-2012; OI Furey, Terry/0000-0001-5546-9672; Stubbs, Lisa/0000-0002-9556-1972; Solovyev, Victor/0000-0001-8885-493X NR 48 TC 130 Z9 1022 U1 2 U2 31 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 J9 NATURE JI Nature PD APR 1 PY 2004 VL 428 IS 6982 BP 529 EP 535 DI 10.1038/nature02399 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 807ZT UT WOS:000220540100034 PM 15057824 ER PT J AU Yang, ZY Kong, WP Huang, Y Roberts, A Murphy, BR Subbarao, K Nabel, GJ AF Yang, ZY Kong, WP Huang, Y Roberts, A Murphy, BR Subbarao, K Nabel, GJ TI A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice SO NATURE LA English DT Article ID ACUTE RESPIRATORY SYNDROME; HONG-KONG; VIRUS; IDENTIFICATION; CHALLENGE; INFECTION; PROTEINS; GENOME; PIGS AB Public health measures have successfully identified and contained outbreaks of the severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV)(1-5), but concerns remain over the possibility of future recurrences. Finding a vaccine for this virus therefore remains a high priority. Here, we show that a DNA vaccine encoding the spike (S) glycoprotein of the SARS-CoV induces T cell and neutralizing antibody responses, as well as protective immunity, in a mouse model. Alternative forms of S were analysed by DNA immunization. These expression vectors induced robust immune responses mediated by CD4 and CD8 cells, as well as significant antibody titres, measured by enzyme-linked immunosorbent assay. Moreover, antibody responses in mice vaccinated with an expression vector encoding a form of S that includes its transmembrane domain elicited neutralizing antibodies. Viral replication was reduced by more than six orders of magnitude in the lungs of mice vaccinated with these S plasmid DNA expression vectors, and protection was mediated by a humoral but not a T-cell-dependent immune mechanism. Gene-based vaccination for the SARS-CoV elicits effective immune responses that generate protective immunity in an animal model. C1 NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA. NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. RP Nabel, GJ (reprint author), NIAID, Vaccine Res Ctr, NIH, Bldg 40,Room 4502,MSC-3005,40 Convent Dr, Bethesda, MD 20892 USA. EM gnabel@nih.gov NR 23 TC 301 Z9 329 U1 0 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 J9 NATURE JI Nature PD APR 1 PY 2004 VL 428 IS 6982 BP 561 EP 564 DI 10.1038/nature02463 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 807ZT UT WOS:000220540100042 PM 15024391 ER PT J AU Morris, RJ Liu, YP Marles, L Yang, ZX Trempus, C Li, SL Lin, JS Sawicki, JA Cotsarelis, G AF Morris, RJ Liu, YP Marles, L Yang, ZX Trempus, C Li, SL Lin, JS Sawicki, JA Cotsarelis, G TI Capturing and profiling adult hair follicle stem cells SO NATURE BIOTECHNOLOGY LA English DT Article ID LABEL-RETAINING CELLS; DIFFERENTIAL EXPRESSION; BASAL CELLS; MOUSE SKIN; BULGE; GROWTH; KERATINOCYTES; EPIDERMIS; INDUCTION; SYSTEM AB The hair follicle bulge possesses putative epithelial stem cells. Characterization of these cells has been hampered by the inability to target bulge cells genetically. Here, we use a Keratin1-15 (Krt1-15, also known as K15) promoter to target mouse bulge cells with an inducible Cre recombinase construct or with the gene encoding enhanced green fluorescent protein (EGFP), which allow for lineage analysis and for isolation of the cells. We show that bulge cells in adult mice generate all epithelial cell types within the intact follicle and hair during normal hair follicle cycling. After isolation, adult Krt1-15-EGFP-positive cells reconstituted all components of the cutaneous epithelium and had a higher proliferative potential than Krt1-15-EGFP-negative cells. Genetic profiling of hair follicle stem cells revealed several known and unknown receptors and signaling pathways important for maintaining the stem cell phenotype. Ultimately, these findings provide potential targets for the treatment of hair loss and other disorders of skin and hair. C1 Univ Penn, Sch Med, Dept Dermatol, Kligman Labs, Philadelphia, PA 19104 USA. Columbia Univ Coll Phys & Surg, Dept Dermatol, New York, NY 10032 USA. Columbia Univ Coll Phys & Surg, Dept Pathol, New York, NY 10032 USA. NIEHS, Canc Biol Grp, Natl Ctr Toxicogenom, Res Triangle Pk, NC 27709 USA. Lankenau Inst Med Res, Wynnewood, PA 19096 USA. RP Cotsarelis, G (reprint author), Univ Penn, Sch Med, Dept Dermatol, Kligman Labs, Philadelphia, PA 19104 USA. EM cotsarel@mail.med.upenn.edu RI Lin, Jamie/F-4278-2015 OI Lin, Jamie/0000-0002-7073-9658 FU NCI NIH HHS [CA97957]; NIAMS NIH HHS [AR46837] NR 45 TC 725 Z9 768 U1 5 U2 49 PU NATURE PUBLISHING GROUP PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1087-0156 J9 NAT BIOTECHNOL JI Nat. Biotechnol. PD APR PY 2004 VL 22 IS 4 BP 411 EP 417 DI 10.1038/nbt950 PG 7 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 809AR UT WOS:000220610100029 PM 15024388 ER PT J AU Polishchuk, R Di Pentima, A Lippincott-Schwartz, J AF Polishchuk, R Di Pentima, A Lippincott-Schwartz, J TI Delivery of raft-associated, GPI-anchored proteins to the apical surface of polarized MDCK cells by a transcytotic pathway SO NATURE CELL BIOLOGY LA English DT Article ID POLYMERIC IMMUNOGLOBULIN RECEPTOR; TRANS-GOLGI NETWORK; EPITHELIAL-CELLS; PLASMA-MEMBRANE; BASOLATERAL MEMBRANE; RECYCLING ENDOSOMES; CYTOPLASMIC DOMAIN; EXOCYTIC PATHWAY; LIVING CELLS; N-GLYCANS AB Epithelial cell polarity depends on mechanisms for targeting proteins to different plasma membrane domains. Here, we dissect the pathway for apical delivery of several raft-associated, glycosyl phosphatidylinositol (GPI)-anchored proteins in polarized MDCK cells using live-cell imaging and selective inhibition of apical or basolateral exocytosis. Rather than trafficking directly from the trans-Golgi network (TGN) to the apical plasma membrane as previously thought, the GPI-anchored proteins followed an indirect, transcytotic route. They first exited the TGN in membrane-bound carriers that also contained basolateral cargo, although the two cargoes were laterally segregated. The carriers were then targeted to and fused with a zone of lateral plasma membrane adjacent to tight junctions that is known to contain the exocyst. Thereafter, the GPI-anchored proteins, but not basolateral cargo, were rapidly internalized, together with endocytic tracer, into clathrin-free transport intermediates that transcytosed to the apical plasma membrane. Thus, apical sorting of these GPI-anchored proteins occurs at the plasma membrane, rather than at the TGN. C1 NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Santa Maria Imbaro, Chieti, Italy. RP Lippincott-Schwartz, J (reprint author), NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. EM jlippin@helix.nih.gov FU Telethon [GTF03006] NR 50 TC 146 Z9 150 U1 0 U2 2 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1465-7392 J9 NAT CELL BIOL JI Nat. Cell Biol. PD APR PY 2004 VL 6 IS 4 BP 297 EP 307 DI 10.1038/ncb1109 PG 11 WC Cell Biology SC Cell Biology GA 808SA UT WOS:000220587600011 PM 15048124 ER PT J AU Biesecker, LG AF Biesecker, LG TI Phenotype matters SO NATURE GENETICS LA English DT Editorial Material AB The association of diseases with genes is complex, even among mendelian disorders. A new study shows that mutations in the gene encoding filamin B (FLNB) cause four distinct disorders of human skeletal development. C1 NHGRI, NIH, Bethesda, MD 20892 USA. RP Biesecker, LG (reprint author), NHGRI, NIH, Bethesda, MD 20892 USA. EM leslieb@helix.nih.gov NR 6 TC 8 Z9 8 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD APR PY 2004 VL 36 IS 4 BP 323 EP 324 DI 10.1038/ng0404-323 PG 3 WC Genetics & Heredity SC Genetics & Heredity GA 809OY UT WOS:000220647200006 PM 15054484 ER PT J AU Bulavin, DV Phillips, C Nannenga, B Timofeev, O Donehower, LA Anderson, CW Appella, E Fornace, AJ AF Bulavin, DV Phillips, C Nannenga, B Timofeev, O Donehower, LA Anderson, CW Appella, E Fornace, AJ TI Inactivation of the Wip1 phosphatase inhibits mammary tumorigenesis through p38 MAPK-mediated activation of the p16(Ink4a)-p19(Arf) pathway SO NATURE GENETICS LA English DT Article ID BREAST-CANCER; CYCLIN D1; TRANSGENIC MICE; GENE PROMOTER; UV-RADIATION; AMPLIFICATION; EXPRESSION; KINASE; PPM1D; P53 AB Modulation of tumor suppressor activities may provide new opportunities for cancer therapy. Here we show that disruption of the gene Ppm1d encoding Wip1 phosphatase activated the p53 and p16 ( also called Ink4a) p19 ( also called ARF) pathways through p38 MAPK signaling and suppressed in vitro transformation of mouse embryo fibroblasts ( MEFs) by oncogenes. Disruption of the gene Cdkn2a ( encoding p16 and p19), but not of Trp53 ( encoding p53), reconstituted cell transformation in Ppm1d - null MEFs. In vivo, deletion of Ppm1d in mice bearing mouse mammary tumor virus ( MMTV) promoter driven oncogenes Erbb2 ( also called c - neu) or Hras1 impaired mammary carcinogenesis, whereas reduced expression of p16 and p19 by methylation-induced silencing or inactivation of p38 MAPK correlated with tumor appearance. We conclude that inactivation or depletion of the Wip1 phosphatase with resultant p38 MAPK activation suppresses tumor appearance by modulating the Cdkn2a tumor-suppressor locus. C1 NCI, Gene Response Sect, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA. Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA. NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Bulavin, DV (reprint author), NCI, Gene Response Sect, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM bulavin@nih.gov RI Fornace, Albert/A-7407-2008 OI Fornace, Albert/0000-0001-9695-085X NR 41 TC 271 Z9 284 U1 1 U2 15 PU NATURE PUBLISHING GROUP PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD APR PY 2004 VL 36 IS 4 BP 343 EP 350 DI 10.1038/ng1317 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA 809OY UT WOS:000220647200017 PM 14991053 ER PT J AU Ng, D Thakker, N Corcoran, CM Donnai, D Perveen, R Schneider, A Hadley, DW Tifft, C Zhang, LQ Wilkie, AOM van der Smagt, JJ Gorlin, RJ Burgess, SM Bardwell, VJ Black, GCM Biesecker, LG AF Ng, D Thakker, N Corcoran, CM Donnai, D Perveen, R Schneider, A Hadley, DW Tifft, C Zhang, LQ Wilkie, AOM van der Smagt, JJ Gorlin, RJ Burgess, SM Bardwell, VJ Black, GCM Biesecker, LG TI Oculofaciocardiodental and Lenz microphthalmia syndromes result from distinct classes of mutations in BCOR SO NATURE GENETICS LA English DT Article ID X-CHROMOSOME INACTIVATION; GERMINAL-CENTER FORMATION; DENTAL OFCD SYNDROME; B-CELL LYMPHOMAS; GENE; BCL-6; TRANSLOCATIONS; LOCALIZATION; INFLAMMATION; COREPRESSOR AB Lenz microphthalmia is inherited in an X-linked recessive pattern and comprises microphthalmia, mental retardation, and skeletal and other anomalies. Two loci associated with this syndrome, MAA ( microphthalmia with associated anomalies) and MAA2, are situated respectively at Xq27-q28 (refs. 1,2) and Xp11.4-p21.2 (ref. 3). We identified a substitution, nt 254C --> T; P85L, in BCOR (encoding BCL-6-interacting corepressor, BCOR 4) in affected males from the family with Lenz syndrome previously used to identify the MAA2 locus 3. Oculofaciocardiodental syndrome (OFCD; OMIM 300166) is inherited in an X-linked dominant pattern with presumed male lethality and comprises microphthalmia, congenital cataracts, radiculomegaly, and cardiac and digital abnormalities. Given their phenotypic overlap, we proposed that OFCD and MAA2 - associated Lenz microphthalmia were allelic, and we found different frameshift, deletion and nonsense mutations in BCOR in seven families affected with OFCD. Like wild-type BCOR, BCOR P85L and an OFCD-mutant form of BCOR can interact with BCL-6 and efficiently repress transcription. This indicates that these syndromes are likely to result from defects in alternative functions of BCOR, such as interactions with transcriptional partners other than BCL-6. We cloned the zebrafish (Danio rerio) ortholog of BCOR and found that knock-down of this ortholog caused developmental perturbations of the eye, skeleton and central nervous system consistent with the human syndromes, confirming that BCOR is a key transcriptional regulator during early embryogenesis. C1 St Marys Hosp, Acad Unit Med Genet, Manchester M13 0JH, Lancs, England. St Marys Hosp, Reg Genet Serv, Manchester M13 0JH, Lancs, England. NHGRI, Genet Dis Res Branch, NIH, Bethesda, MD 20892 USA. Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA. Univ Minnesota, Ctr Canc, Minneapolis, MN USA. Albert Einstein Med Ctr, Dept Genet, Philadelphia, PA 19141 USA. NHGRI, Med Genet Branch, NIH, Bethesda, MD 20892 USA. NIDDKD, Genet Dev & Dis Res Branch, NIH, Bethesda, MD 20892 USA. Childrens Natl Med Ctr, Div Genet, Washington, DC 20010 USA. John Radcliffe Hosp, Weatherall Inst Mol Med, Oxford OX3 9DS, England. Univ Utrecht, Med Ctr, Dept Med Genet, NL-3508 AB Utrecht, Netherlands. Univ Minnesota, Dept Oral Pathol & Genet, Sch Dent, Minneapolis, MN USA. NHGRI, Genome Technol Branch, NIH, Bethesda, MD 20892 USA. Ctr Mol Med, Manchester, Lancs, England. Cent Manchester & Manchester Childrens Univ Hosp, Manchester Royal Eye Hosp, Manchester, Lancs, England. RP Black, GCM (reprint author), St Marys Hosp, Acad Unit Med Genet, Manchester M13 0JH, Lancs, England. EM gblack@man.ac.uk OI Burgess, Shawn/0000-0003-1147-0596; Wilkie, Andrew/0000-0002-2972-5481; Black, Graeme/0000-0001-8727-6592 FU NCI NIH HHS [R01 CA071540] NR 26 TC 143 Z9 147 U1 1 U2 4 PU NATURE PUBLISHING GROUP PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1061-4036 J9 NAT GENET JI Nature Genet. PD APR PY 2004 VL 36 IS 4 BP 411 EP 416 DI 10.1038/ng1321 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA 809OY UT WOS:000220647200027 PM 15004558 ER PT J AU Guo, ZM Linn, JF Wu, GJ Anzick, SL Eisenberger, CF Halachmi, S Cohen, Y Fomenkov, A Hoque, MO Okami, K Steiner, G Engles, JM Osada, M Moon, C Ratovitski, E Trent, JM Meltzer, PS Westra, WH Kiemeney, LA Schoenberg, MP Sidransky, D Trink, B AF Guo, ZM Linn, JF Wu, GJ Anzick, SL Eisenberger, CF Halachmi, S Cohen, Y Fomenkov, A Hoque, MO Okami, K Steiner, G Engles, JM Osada, M Moon, C Ratovitski, E Trent, JM Meltzer, PS Westra, WH Kiemeney, LA Schoenberg, MP Sidransky, D Trink, B TI CDC91L1 (PIG-U) is a newly discovered oncogene in human bladder cancer SO NATURE MEDICINE LA English DT Article ID COMPARATIVE GENOMIC HYBRIDIZATION; TUMOR-SUPPRESSOR GENES; B-CELL LYMPHOMAS; BREAST-CANCER; COPY NUMBER; DNA AMPLIFICATION; LONG ARM; MYC; SEQUENCES; RECEPTOR AB Genomic amplification at 20q11-13 is a common event in human cancers. We isolated a germline translocation breakpoint at 20q11 from a bladder cancer patient. We identified CDC91L1, the gene encoding CDC91L1 (also called phosphatidylinositol glycan class U (PIG-U), a transamidase complex unit in the glycosylphosphatidylinositol (GPI) anchoring pathway), as the only gene whose expression was affected by the translocation. CDC91L1 was amplified and overexpressed in about one-third of bladder cancer cell lines and primary tumors, as well as in oncogenic uroepithelial cells transformed with human papillomavirus (HPV) E7. Forced overexpression of CDC91L1 malignantly transformed NIH3T3 cells in vitro and in vivo. Overexpression of CDC91L1 also resulted in upregulation of the urokinase receptor (uPAR), a GPI-anchored protein, and in turn increased STAT-3 phosphorylation in bladder cancer cells. Our findings suggest that CDC91L1 is an oncogene in bladder cancer, and implicate the GPI anchoring system as a potential oncogenic pathway and therapeutic target in human cancers. C1 Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Div Head & Neck Canc Res, Baltimore, MD 21205 USA. NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Med, Dept Dermatol, Baltimore, MD 21205 USA. Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21205 USA. Univ Med Ctr, Dept Urol, Dept Epidemiol & Biostat, NL-6500 HB Nijmegen, Netherlands. Johns Hopkins Univ Hosp, Inst Med, Dept Urol, James Buchanan Brady Urol Inst, Baltimore, MD 21205 USA. RP Sidransky, D (reprint author), Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Div Head & Neck Canc Res, Baltimore, MD 21205 USA. EM dsidrans@jhmi.edu; btrink@jhmi.edu RI Kiemeney, Lambertus/D-3357-2009 OI Kiemeney, Lambertus/0000-0002-2368-1326 FU NCI NIH HHS [5P01CA77664] NR 34 TC 42 Z9 42 U1 0 U2 3 PU NATURE PUBLISHING GROUP PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1078-8956 J9 NAT MED JI Nat. Med. PD APR PY 2004 VL 10 IS 4 BP 374 EP 381 DI 10.1038/nm1010 PG 8 WC Biochemistry & Molecular Biology; Cell Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Cell Biology; Research & Experimental Medicine GA 808RU UT WOS:000220587000028 PM 15034568 ER PT J AU Ransohoff, DF AF Ransohoff, DF TI Opinion - Rules of evidence for cancer molecular-marker discovery and validation SO NATURE REVIEWS CANCER LA English DT Article ID DIAGNOSTIC-TESTS; BREAST-CANCER; METHODOLOGICAL STANDARDS; OVARIAN-CANCER; DNA MICROARRAYS; PREDICTION; MEDICINE; SERUM; BIAS; EPIDEMIOLOGY AB According to some claims, molecular markers are set to revolutionize the process of evaluating prognosis and diagnosis for cancer. Research about cancer markers has, however, been characterized by inflated expectations, followed by disappointment when original results can not be reproduced. Even now, disappointment might be expected, in part because rules of evidence to assess the validity of studies about diagnosis and prognosis are both underdeveloped and not routinely applied. What challenges are involved in assessing studies and how might problems be avoided so as to realize the full potential of this emerging technology? C1 Univ N Carolina, Dept Med, Chapel Hill, NC 27599 USA. Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27599 USA. NCI, Div Canc Prevent, NIH, Bethesda, MD 20892 USA. RP Ransohoff, DF (reprint author), Univ N Carolina, Dept Med, CB 7080,Bioinformat Bldg 4103, Chapel Hill, NC 27599 USA. EM ransohof@med.unc.edu NR 58 TC 343 Z9 351 U1 1 U2 9 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1474-175X J9 NAT REV CANCER JI Nat. Rev. Cancer PD APR PY 2004 VL 4 IS 4 BP 309 EP 314 DI 10.1038/nrc1322 PG 6 WC Oncology SC Oncology GA 808GZ UT WOS:000220558900015 PM 15057290 ER PT J AU Sharp, RR Yudell, MA Wilson, SH AF Sharp, RR Yudell, MA Wilson, SH TI Shaping science policy in the age of genomics SO NATURE REVIEWS GENETICS LA English DT Review ID HUMAN GENETIC MATERIAL; PUBLIC-POLICY; ENVIRONMENTAL-HEALTH; SOCIAL IMPLICATIONS; MEDICINE; DISCRIMINATION; ASILOMAR; ETHICS; EUROPE; US AB The potential of genomic technologies for improving the diagnosis and treatment of many human diseases will not be fully realized until several ethical, legal and social issues are addressed by effective science policy. We believe that more widespread public debate and subsequent policy action are urgently required. Here, we discuss several mechanisms by which this might occur. We propose an independent genome policy organization as an additional approach to promoting informed science policy in the age of genomics. C1 Columbia Univ, Mailman Sch Publ Hlth, Dept Sociomed Sci, Ctr Hist & Eth Publ Hlth, New York, NY 10032 USA. Baylor Coll Med, Ctr Med Eth & Hlth Policy, Houston, TX 77030 USA. NIEHS, NIH, Res Triangle Pk, NC 27709 USA. RP Yudell, MA (reprint author), Columbia Univ, Mailman Sch Publ Hlth, Dept Sociomed Sci, Ctr Hist & Eth Publ Hlth, 722 W 168th St, New York, NY 10032 USA. EM may16@columbia.edu NR 85 TC 7 Z9 7 U1 0 U2 2 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1471-0056 J9 NAT REV GENET JI Nat. Rev. Genet. PD APR PY 2004 VL 5 IS 4 BP 311 EP U1 DI 10.1038/nrg1320 PG 6 WC Genetics & Heredity SC Genetics & Heredity GA 808HC UT WOS:000220559200017 PM 15134075 ER PT J AU Schiller, JT Davies, P AF Schiller, JT Davies, P TI Science and society - Delivering on the promise: HPV vaccines and cervical cancer SO NATURE REVIEWS MICROBIOLOGY LA English DT Review ID VIRUS-LIKE PARTICLES; HUMAN-PAPILLOMAVIRUS INFECTION; NEUTRALIZING ANTIBODIES; ADULT VOLUNTEERS; CAPSID PROTEIN; RISK-FACTORS; WOMEN; IMMUNIZATION; IMMUNOGENICITY; VACCINATION AB Human papillomavirus (HPV) vaccines for the prevention of cervical cancer have produced encouraging results in recent clinical trials, and expectations are high that one or more vaccines will be licensed for commercial distribution within the next five years. The availability of an HPV vaccine would raise several implementation issues that must be addressed if the vaccine is to achieve the coverage necessary to significantly reduce the incidence of cervical cancer. The main implementation issues will differ between developing countries, where cervical cancer is often a leading cause of cancer deaths in women, and developed countries, where cervical cancer screening programmes have already substantially reduced the number of deaths from cervical cancer. C1 NCI, NIH, Bethesda, MD 20892 USA. European Cerv Canc Assoc, Lyon, France. RP Schiller, JT (reprint author), NCI, NIH, Bethesda, MD 20892 USA. EM schillej@dc37a.nci.nih.gov NR 43 TC 72 Z9 80 U1 1 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1740-1526 J9 NAT REV MICROBIOL JI Nat. Rev. Microbiol. PD APR PY 2004 VL 2 IS 4 BP 343 EP 347 DI 10.1038/nrmicro867 PG 5 WC Microbiology SC Microbiology GA 810OY UT WOS:000220714800017 PM 15031733 ER PT J AU Thompson, PR Wang, DX Wang, L Fulco, M Pediconi, N Zhang, DZ An, WJ Ge, QY Roeder, RG Wong, JM Levrero, M Sartorelli, V Cotter, RJ Cole, PA AF Thompson, PR Wang, DX Wang, L Fulco, M Pediconi, N Zhang, DZ An, WJ Ge, QY Roeder, RG Wong, JM Levrero, M Sartorelli, V Cotter, RJ Cole, PA TI Regulation of the p300 HAT domain via a novel activation loop SO NATURE STRUCTURAL & MOLECULAR BIOLOGY LA English DT Article ID HISTONE ACETYLTRANSFERASE ACTIVITY; CREB-BINDING-PROTEIN; TRANSCRIPTIONAL REPRESSION; CBP; COACTIVATOR; PHOSPHORYLATION; ONCOPROTEIN; METHYLATION; ACETYLATION; INHIBITORS AB The transcriptional coactivator p300 is a histone acetyltransferase ( HAT) whose function is critical for regulating gene expression in mammalian cells. However, the molecular events that regulate p300 HAT activity are poorly understood. We evaluated autoacetylation of the p300 HAT protein domain to determine its function. Using expressed protein ligation, the p300 HAT protein domain was generated in hypoacetylated form and found to have reduced catalytic activity. This basal catalytic rate was stimulated by autoacetylation of several key lysine sites within an apparent activation loop motif. This post-translational modification and catalytic regulation of p300 HAT activity is conceptually analogous to the activation of most protein kinases by autophosphorylation. We therefore propose that this autoregulatory loop could influence the impact of p300 on a wide variety of signaling and transcriptional events. C1 Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA. NIAMSD, Muscle Gene Express Grp, Muscle Biol Lab, Intramural Res Program,NIH, Bethesda, MD 20892 USA. Univ Roma La Sapienza, Policlin Umberto I, Fdn Andrea Cesalpino, Gene Express Lab, I-00161 Rome, Italy. Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. Rockefeller Univ, Biochem & Mol Biol Lab, New York, NY 10021 USA. Cell Signaling Technol, Beverly, MA 01915 USA. Regina Elena Inst Canc Res, Dept Mol Oncogenesis, I-00158 Rome, Italy. RP Cole, PA (reprint author), Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA. EM pcole@jhmi.edu NR 36 TC 209 Z9 214 U1 4 U2 12 PU NATURE PUBLISHING GROUP PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 1545-9985 J9 NAT STRUCT MOL BIOL JI Nat. Struct. Mol. Biol. PD APR PY 2004 VL 11 IS 4 BP 308 EP 315 DI 10.1038/nsmb740 PG 8 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 810GN UT WOS:000220692900013 PM 15004546 ER PT J AU Kolker, DE Vitaterna, MH Fruechte, EM Takahashi, JS Turek, FW AF Kolker, DE Vitaterna, MH Fruechte, EM Takahashi, JS Turek, FW TI Effects of age on circadian rhythms are similar in wild-type and heterozygous Clock mutant mice SO NEUROBIOLOGY OF AGING LA English DT Article DE aging; circadian rhythm; clock; clock mutation ID SUPRACHIASMATIC NUCLEUS NEURONS; GOLDEN-HAMSTERS; AGING ALTERS; PERIOD; YOUNG; GENE; MPER2; LIGHT; RATS; EXPRESSION AB The amplitudes of many circadian rhythms, at the behavioral, physiological, cellular, and biochemical levels, decrease with advanced age. Previous studies suggest that the amplitude of the central circadian pacemaker is decreased in old animals. Recently it has been reported that expression of several circadian clock genes, including Clock, is lower in the master circadian pacemaker of old rodents. To test the hypothesis that decreased activity of a circadian clock gene renders animals more susceptible to the effects of aging, we analyzed the circadian rhythm of locomotor activity in young and old wild-type and heterozygous Clock mutant mice. We found that the effects of age and the Clock mutation were additive. These results indicate that age-related changes in circadian rhythmicity occur equally in wild-type and heterozygous Clock mutants, suggesting that the Clock mutation does not render mice more susceptible to the effects of age on the circadian pacemaker. 0 2003 Elsevier Inc. All rights reserved. C1 Northwestern Univ, Dept Neurobiol & Physiol, Evanston, IL 60208 USA. Howard Hughes Med Inst, Bethesda, MD 20817 USA. RP Kolker, DE (reprint author), NICHHD, 49 Convent Dr,Bldg 49,Room 6A-36,MSC 4510, Bethesda, MD 20892 USA. EM kolkerd@mail.nih.gov RI Takahashi, Joseph/E-8482-2012 OI Takahashi, Joseph/0000-0003-0384-8878 FU Howard Hughes Medical Institute; NIA NIH HHS [P01 AG011412, P01-AG-11412, R01 AG018200, R01-AG-18200] NR 39 TC 21 Z9 25 U1 0 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD APR PY 2004 VL 25 IS 4 BP 517 EP 523 DI 10.1016/j.neurobiolaging.2003.06.007 PG 7 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA 806PX UT WOS:000220446900012 PM 15013573 ER PT J AU Lamar, M Resnick, SM AF Lamar, M Resnick, SM TI Aging and prefrontal functions: dissociating orbitofrontal and dorsolateral abilities SO NEUROBIOLOGY OF AGING LA English DT Article DE orbitofrontal functioning; dorsolateral prefrontal functioning; prefrontal cortex; aging ID AGE-DIFFERENCES; WORKING-MEMORY; FRONTAL-LOBE; OLDER-ADULTS; VISUAL RECOGNITION; DECISION-MAKING; CORTEX; LESIONS; PERFORMANCE; FLUENCY AB This study aimed to determine whether age differentially affects performance on tasks tapping orbitofrontal cortex (OFC) and dorsolateral prefrontal cortex (DLPFC). We administered prefrontal measures to healthy younger (n = 23; age = 28.4 +/- 5.9, education 15.7 +/- 2.6, MMSE = 29.5 +/- 0.6) and older participants (n = 20; age = 69.1 +/- 5.0, education = 15.5 +/- 3.4, MMSE 28.9 +/- 1.5). Groups did not differ on education or mental status, P > 0.05. Tasks thought to involve greater OFC processing included the Iowa Gambling Task and Delayed Match and Non-Match to Sample Tasks. Tasks requiring greater DLPFC processing included Petrides' Self-Ordered Pointing, WAIS-R Digit Span Backward, Letter Fluency, and Months Backward from the Boston Revision of WMS-Mental Control. Composite z-scores were calculated for OFC and DLPFC tasks. A 2 x 2 ANOVA revealed a Group x Task interaction: F(1, 41) = 5.55, P = 0.02, and a Group main effect: F(1, 41) = 12.16, P = 0.001. Follow-up analyses revealed younger adults outperformed older adults on OFC tasks only (younger = 0.37 +/- 0.46, older = -0.43 +/- 0.70; t(41) = 4.5, P < 0.001). Post-hoc analyses of individual tasks confirmed that despite age differences on Petrides' Self-Ordered Pointing, measures requiring relatively greater OFC involvement showed larger effect sizes for age differences. Thus, tasks emphasizing OFC functions appear more sensitive to age effects when directly compared to measures of DLPFC functioning. Reasons for this difference in magnitude may stem from differential aging of prefrontal cortex or differential recruitment of alternative brain regions for successful task completion. (C) 2003 Published by Elsevier Inc. C1 NIA, Lab Personal & Cognit, Ctr Gerontol Res, Baltimore, MD 21224 USA. RP Lamar, M (reprint author), NIA, Lab Personal & Cognit, Ctr Gerontol Res, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. EM me2325@columbia.edu NR 47 TC 69 Z9 75 U1 0 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD APR PY 2004 VL 25 IS 4 BP 553 EP 558 DI 10.1016/j.neurobiolaging.2003.06.005 PG 6 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA 806PX UT WOS:000220446900016 PM 15013577 ER PT J AU Greig, NH Ingram, DK Wang, Y Yu, QS Pepeu, G Sambamurti, K Rogers, J Brossi, A Lahiri, DK AF Greig, NH Ingram, DK Wang, Y Yu, QS Pepeu, G Sambamurti, K Rogers, J Brossi, A Lahiri, DK TI Butyrylcholinesterase: Role in memory and disease progression SO NEUROBIOLOGY OF AGING LA English DT Meeting Abstract CT 8th International Montreal-Springfield Symposium on Advances in Alzheimer Therapy CY APR 14-17, 2004 CL Montreal, CANADA C1 NIA, NIH, Baltimore, MD 21224 USA. Univ Florence, Florence, Italy. Med Univ S Carolina, Charleston, SC 29425 USA. Harvard Univ, Sch Med, Boston, MA USA. Univ N Carolina, Chapel Hill, NC USA. Indiana Univ, Sch Med, Indianapolis, IN USA. EM greign@irpns.grc.nia.nih.gov NR 0 TC 2 Z9 2 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD APR PY 2004 VL 25 SU 1 MA 45 BP S13 EP S13 PG 1 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA 808SW UT WOS:000220589800046 ER PT J AU Hardy, J AF Hardy, J TI Concentrations matter: Genetic evidence that the amounts of pathogenic proteins matter in the pathogeneses of Alzheimer and Parkinson diseases SO NEUROBIOLOGY OF AGING LA English DT Meeting Abstract CT 8th International Montreal-Springfield Symposium on Advances in Alzheimer Therapy CY APR 14-17, 2004 CL Montreal, CANADA C1 NIA, Neurogenet Lab, NIH, Bethesda, MD 20892 USA. EM hardyj@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD APR PY 2004 VL 25 SU 1 MA 46 BP S13 EP S13 PG 1 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA 808SW UT WOS:000220589800047 ER PT J AU Lahiri, DK Bailey, J Alley, G Chen, D Farlow, M Greig, NH AF Lahiri, DK Bailey, J Alley, G Chen, D Farlow, M Greig, NH TI Recent advances on cholinesterase inhibitors and amyloid-beta protein: Implications in Alzheimer disease SO NEUROBIOLOGY OF AGING LA English DT Meeting Abstract CT 8th International Montreal-Springfield Symposium on Advances in Alzheimer Therapy CY APR 14-17, 2004 CL Montreal, CANADA C1 Indiana Univ, Sch Med, Dept Psychiat, Indianapolis, IN 46202 USA. Indiana Univ, Sch Med, Dept Neurol, Indianapolis, IN 46202 USA. NIA, NIH, Baltimore, MD 21224 USA. EM dlahiri@iupui.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD APR PY 2004 VL 25 SU 1 MA 55 BP S15 EP S16 PG 2 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA 808SW UT WOS:000220589800056 ER PT J AU Sparks, DL Connor, D Lopez, J Launer, L Petanceska, S Baxter, L Wasser, D Lochhead, J Ziolowski, C Idouraine, A Browne, P Sabbagh, M AF Sparks, DL Connor, D Lopez, J Launer, L Petanceska, S Baxter, L Wasser, D Lochhead, J Ziolowski, C Idouraine, A Browne, P Sabbagh, M TI Benefit of atorvastatin in the treatment of Alzheimer disease SO NEUROBIOLOGY OF AGING LA English DT Meeting Abstract CT 8th International Montreal-Springfield Symposium on Advances in Alzheimer Therapy CY APR 14-17, 2004 CL Montreal, CANADA C1 Sun Hlth Res Inst, Sun City, AZ USA. NIA, NIH, Bethesda, MD 20892 USA. NYU, New York, NY USA. Barrow Neurol Inst, Phoenix, AZ 85013 USA. EM larry.sparks@sunhealth.org NR 0 TC 4 Z9 4 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0197-4580 J9 NEUROBIOL AGING JI Neurobiol. Aging PD APR PY 2004 VL 25 SU 1 MA 86 BP S24 EP S24 PG 1 WC Geriatrics & Gerontology; Neurosciences SC Geriatrics & Gerontology; Neurosciences & Neurology GA 808SW UT WOS:000220589800087 ER PT J AU Lamar, M Yousem, DM Resnick, SM AF Lamar, M Yousem, DM Resnick, SM TI Age differences in orbitofrontal activation: an fMRI investigation of delayed match and nonmatch to sample SO NEUROIMAGE LA English DT Article DE fMRI; orbitofrontal cortex; age-related differences ID ORBITAL FRONTAL-CORTEX; OLDER-ADULTS; TO-SAMPLE; RECOGNITION MEMORY; PERFORMANCE; LESIONS; MONKEYS; BRAIN; CYTOARCHITECTURE; MRI AB Several investigations have suggested that the orbitofrontal cortex (OFC) may be particularly vulnerable to the effects of age-related changes. We recently reported behavioral data indicating greater age differences in orbitofrontal tasks when directly compared to tasks tapping dorsolateral prefrontal functions. The present study was designed to investigate the neural underpinnings of age differences in OFC functioning. Event-related functional magnetic resonance imaging (fMRI) was performed during delayed match and nonmatch to sample tasks, previously shown to differentially activate medial and lateral OFC in young adults. Sixteen healthy younger [age = 26.7(5.6)] and 16 healthy older individuals [age = 69.1 + 5.] with similar levels of education and general cognitive functioning participated in the experiment. Participants chose the stimulus from a pair of stimuli matching a previously viewed target (match to sample) or chose the nontarget item (nonmatch to sample) depending upon a trial-specific instruction word. Consistent with previous studies, SPM99 analyses of the younger age group revealed activation for medial OFC regions during the match task compared to the nonmatch task and lateral OFC activation during the nonmatch task compared to the match task. In contrast, older adults showed prefrontal activation only during the match relative to the nonmatch task and posterior temporal and limbic involvement during the nonmatch relative to the match task. Between-group analyses confirmed within-group results suggesting differential age-related recruitment of prefrontal regions when performing match and nonmatch tasks. Results suggest that OFC recruitment during these cognitive tasks changes with age and should be evaluated within the context of other prefrontal subregions to further define differential age effects on frontal functions. Published by Elsevier Inc. C1 NIA, Lab Personality & Cognit, Ctr Gerontol Res, Baltimore, MD 21224 USA. Johns Hopkins Univ Hosp, Dept Radiol & Radiol Sci, Baltimore, MD 21287 USA. RP Lamar, M (reprint author), Columbia Univ, Dept Psychiat, 1051 Riverside Dr, New York, NY 10032 USA. EM ml2325@columbia.edu NR 35 TC 36 Z9 38 U1 0 U2 3 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR PY 2004 VL 21 IS 4 BP 1368 EP 1376 DI 10.1016/j.neuroimage.2003.11.018 PG 9 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 810SL UT WOS:000220723900016 PM 15050562 ER PT J AU Husain, FT Tagamets, MA Fromm, SJ Braun, AR Horwitz, B AF Husain, FT Tagamets, MA Fromm, SJ Braun, AR Horwitz, B TI Relating neuronal dynamics for auditory object processing to neuroimaging activity: a computational modeling and an fMRI study SO NEUROIMAGE LA English DT Article DE brain; human; primate; neural network; auditory cortex; PET; superior temporal gyrus; working memory ID DORSOLATERAL PREFRONTAL CORTEX; SHORT-TERM-MEMORY; FUNCTIONAL ARCHITECTURE; TEMPORAL CORTEX; SYNAPTIC ACTIVITY; SQUIRREL-MONKEYS; CEREBRAL-CORTEX; WORKING-MEMORY; COMPLEX SOUNDS; VISUAL-CORTEX AB We investigated the neural basis of auditory object processing in the cerebral cortex by combining neural modeling and functional neuro-imaging. We developed a large-scale, neurobiologically realistic network model of auditory pattern recognition that relates the neuronal dynamics of cortical auditory processing of frequency modulated (FM) sweeps to functional neuroimaging data of the type obtained using PET and fMRI. Areas included in the model extend from primary auditory to prefrontal cortex. The electrical activities of the neuronal units of the model were constrained to agree with data from the neurophysiological literature regarding the perception of FM sweeps. We also conducted an fMRI experiment using stimuli and tasks similar to those used in our simulations. The integrated synaptic activity of the neuronal units in each region of the model, convolved with a hemodynamic response function, was used as a correlate of the simulated fMRI activity, and generally agreed with the experimentally observed fMRl data in the brain areas corresponding to the regions of the model. Our results demonstrate that the model is capable of exhibiting the salient features of both electrophysiological neuronal activities and fMRI values that are in agreement with empirically observed data. These findings provide support for our hypotheses concerning how auditory objects are processed by primate neocortex. (C) 2004 Elsevier Inc. All rights reserved. C1 NIDCD, Brain Imaging & Modeling Sect, NIH, Bethesda, MD 20892 USA. Univ Maryland, Sch Med, Funct Neuroimaging Lab, Maryland Psychiat Res Ctr, Baltimore, MD 21201 USA. NIDCD, Language Sect, NIH, Bethesda, MD USA. RP Husain, FT (reprint author), NIDCD, Brain Imaging & Modeling Sect, NIH, Bldg 10,Room 6C420,MSC 1591,9000 Rockville Pike, Bethesda, MD 20892 USA. EM husainf@nidcd.nih.gov NR 85 TC 55 Z9 58 U1 1 U2 5 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1053-8119 J9 NEUROIMAGE JI Neuroimage PD APR PY 2004 VL 21 IS 4 BP 1701 EP 1720 DI 10.1016/j.neuroimage.2003.11.012 PG 20 WC Neurosciences; Neuroimaging; Radiology, Nuclear Medicine & Medical Imaging SC Neurosciences & Neurology; Radiology, Nuclear Medicine & Medical Imaging GA 810SL UT WOS:000220723900046 PM 15050592 ER PT J AU Yamada, S Brauer, FS Colohan, ART Won, DJ Siddiqi, J Johnson, WD Yamada, SM Rouse, GA Lonser, RR Iacono, RP Mandybur, GT AF Yamada, S Brauer, FS Colohan, ART Won, DJ Siddiqi, J Johnson, WD Yamada, SM Rouse, GA Lonser, RR Iacono, RP Mandybur, GT TI Concept of arteriovenous malformation compartments and surgical management SO NEUROLOGICAL RESEARCH LA English DT Article DE AVM; compartments; AVM resection; compartmental dissection ID EMBOLIZATION; RADIOSURGERY; SURGERY; MICROSURGERY; BRAIN; FLOW AB Cerebral AVMs are known to be a Source of intracranial hemorrhages and epileptic seizures. Their natural history indicates approximately 15% mortality and 35% morbidity over a 15-year period. This significant mortality and morbidity mandates a need for satisfactory treatment of this entity, ideally by elimination of AVMs. Microsurgical resection, endovascular embolization and radiosurgery (irradiation) are the three effective modes of treatment Currently available. However, no objective criteria have been established for which mode(s) of treatment should be selected for individual patients with AVMs. Considering the complexity of AVMs and variable conditions of individual patients, neurosurgeons, intravascular interventionalists and radiosurgeons must make their own decisions on how to treat each patient based on their experience. In practice, treatment of small AVMs in non-functional areas is favored equally by each of these specialists, while they tend to avoid treatment of large AVMs, particularly those in functional areas of the brain. The authors report the surgical intervention of large AVMs, including those located in functional areas of the hemisphere by special techniques. One can demonstrate AVM compartments by using angiography and with the aid of color Doppler ultrasonography, each compartment can be outlined and dissected individually until all the compartments are isolated without causing any damage to the surrounding brain and the entire AVM is rendered shrunken and then removed. The concept of compartmental treatment of AVMs may be applied in the future to radiosurgery and intravascular embolization of large AVMs. C1 Loma Linda Univ, Sch Med, Dept Neurosurg, Loma Linda, CA 92354 USA. Loma Linda Univ, Sch Med, Dept Anesthesiol, Loma Linda, CA 92354 USA. Loma Linda Univ, Sch Med, Dept Radiol, Loma Linda, CA 92354 USA. Arrowhead Reg Med Ctr, Dept Neurosurg, Colton, CA USA. NINDS, Surg Neurol Branch, NIH, Bethesda, MD 20892 USA. Univ Mississippi, Med Ctr, Dept Neurosurg, Jackson, MS 39216 USA. Nippon Med Coll, Dept Neurosurg, Tokyo 113, Japan. RP Yamada, S (reprint author), Loma Linda Univ, Sch Med, Dept Neurosurg, 11234 Anderson St, Loma Linda, CA 92354 USA. EM yamada1000@worldnet.att.net NR 42 TC 16 Z9 16 U1 0 U2 1 PU MANEY PUBLISHING PI LEEDS PA HUDSON RD, LEEDS LS9 7DL, ENGLAND SN 0161-6412 J9 NEUROL RES JI Neurol. Res. PD APR PY 2004 VL 26 IS 3 BP 288 EP 300 DI 10.1179/016164014225013987 PG 13 WC Clinical Neurology; Neurosciences SC Neurosciences & Neurology GA 818XL UT WOS:000221277700007 PM 15142322 ER PT J AU Williams, WM Stadtman, ER Moskovitz, J AF Williams, WM Stadtman, ER Moskovitz, J TI Ageing and exposure to oxidative stress in vivo differentially affect cellular levels of PrPc in mouse cerebral microvessels and brain parenchyma SO NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY LA English DT Article DE ageing; brain; hyperoxia; microvessel; mouse; oxidative stress; PrPc ID METHIONINE SULFOXIDE REDUCTASE; CREUTZFELDT-JAKOB-DISEASE; NORMAL PRION PROTEIN; ALZHEIMERS-DISEASE; CELLS; GENE; POLYMORPHISM; EXPRESSION; POPULATION; CAVEOLAE AB The biological function of cellular prion protein PrPc has not been established, despite hi vitro studies suggesting antioxidant activity or link to signal transduction pathways. In this study, mice were exposed to hyperoxia to establish whether oxidative stress affected prion expression in vivo. C57BI/6J mice aged 6, 18, and 24 months. maintained under normoxic conditions, exhibited age-related increases in PrPc in both cerebral microvessels and in microvessel-depleted brain homogenate. We demonstrate that PrPc is differentially affected by exposure to hyperoxia hi vivo for 1. (24 h) or 2 (48 h) days, or for 1 day hyperoxia, followed by 1 day normoxia. Brain parenchymal cells from 6-month-old mice exposed to 1 day hyperoxia showed elevation of a glycosylated similar to36 kDa form, whereas in 24-month-old mice cellular prion level was substantially reduced. Extending hyperoxia from 1 to 2 days resulted in significantly reduced PrPc level, regardless of age. Parenchymal PrPc is substantially elevated in 6-month-old mice. but declines in 18- and 24-month-old animals following 1 day hyperoxia. By contrast, PrPc content in cerebral microvessels from 6-month-old mice declined after a 2 day exposure to hyperoxia, white microvessels from 24-month-old brains showed elevated prion levels 24 h after hyperoxia. Moreover, unglycosylated 25-30 kDa PrPc, and a previously undescribed 50-64 kDa band containing at least some glycosylated protein. predominated in microvessels with lesser content of the glycosylated similar to36 kDa form. Cellular content. of these unglycosylated forms was correlated with age, while the response to hyperoxia was evident in both unglycosylated and glycosylated forms of the protein following 1 and 2 day exposures. The observed elevation of the 25-30 and 50-64 kDa bands of microvessel PrPc is not sustainable following 1 day hyperoxia, but returns to near normoxic levels within 24 h after hyperoxia. We also show in a knockout mouse for methionine sulfoxide reductase (MsrA). the enzyme responsible for reducing methionine sulfoxide back to methionine. and a regulator of cellular antioxidant defence, that following hyperoxia brain PrPc in the null mutant is elevated relative to PrPc content in the parent strain. Our results show up-regulated PrPc expression or reduced turnover in response to age-related, and hyperoxia-induced oxidative stress. C1 NHLBI, Biochem Lab, NIH, Bethesda, MD 20892 USA. RP Williams, WM (reprint author), Vet Adm Med Ctr, Res Dept, 151W 10701 East Blvd, Cleveland, OH 44106 USA. EM wmw5@po.cwru.edu NR 28 TC 20 Z9 20 U1 0 U2 0 PU BLACKWELL PUBLISHING LTD PI OXFORD PA 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND SN 0305-1846 J9 NEUROPATH APPL NEURO JI Neuropathol. Appl. Neurobiol. PD APR PY 2004 VL 30 IS 2 BP 161 EP 168 DI 10.1111/j.1365-2990.2004.00523.x PG 8 WC Clinical Neurology; Neurosciences; Pathology SC Neurosciences & Neurology; Pathology GA 814LK UT WOS:000220976000007 PM 15043713 ER PT J AU Honse, Y Ren, H Lipsky, RH Peoples, RW AF Honse, Y Ren, H Lipsky, RH Peoples, RW TI Sites in the fourth membrane-associated domain regulate alcohol sensitivity of the NMDA receptor SO NEUROPHARMACOLOGY LA English DT Article DE glutamate receptor; ethanol; site-directed mutagenesis; patch-clamp ID D-ASPARTATE RECEPTORS; ACTIVATED ION CURRENT; ETHANOL INHIBITION; HIPPOCAMPAL-NEURONS; POTASSIUM CHANNEL; GLYCINE RECEPTORS; SINGLE-CHANNEL; K+ CHANNEL; CURRENTS; SUBUNITS AB N-methyl-n-aspartate (NMDA) receptors are important target sites of alcohol action in the central nervous system. Alcohol inhibits NMDA receptor Current by an action on ion channel gating, apparently through a direct action on a region of the NMDA receptor accessible from the extracellular environment. Our previous studies have revealed an important role for a methionine residue (Met823) in membrane-associated domain 4 (M4) of the NR2A subunit in channel gating as well as alcohol sensitivity of the NMDA receptor. The role of sites in M4 of the NMDA receptor NR2A Subunit adjacent to Met823 was investigated using tryptophan-scanning mutagenesis and electrophysiological recording. Receptors containing NR1 and NUA(V820W) or NR2A(M817W) Mutant Subunits expressed in HEK 293 cells were not functional. The mutation Ala826Trp modified apparent desensitization. and the mutations Ala825Trp and Ala826Trp changed the mean open time of the channel as determined by fluetuation analysis. In addition, the mutations Tyr822Trp and Ala825Trp significantly altered the concentration-response curves for ethanol inhibition. The changes in mean open time did not appear to be able to account for the observed differences in ethanol sensitivity. These results indicate that this region in M4 of the NR2A Subunit may be involved in the action of alcohol. Published by Elsevier Ltd. C1 NIAAA, Unit Cellular Neuropharmacol, Lab Mol Cellular Neurobiol, NIH, Bethesda, MD 20892 USA. NIAAA, Unit Cellular Neuropharmacol, Neurogenet Lab, NIH, Bethesda, MD 20892 USA. RP Honse, Y (reprint author), NIAAA, Lab Integrat Neurosci, NIH, Pk 5 Bldg,Room 158,12420 Parklawn Dr,MSC 8115, Bethesda, MD 20892 USA. EM yhonse@mail.nih.gov OI Lipsky, Robert/0000-0001-7753-1473 NR 31 TC 32 Z9 33 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0028-3908 J9 NEUROPHARMACOLOGY JI Neuropharmacology PD APR PY 2004 VL 46 IS 5 BP 647 EP 654 DI 10.1016/j.neuropharm.2003.11.006 PG 8 WC Neurosciences; Pharmacology & Pharmacy SC Neurosciences & Neurology; Pharmacology & Pharmacy GA 809CB UT WOS:000220613700005 PM 14996542 ER PT J AU Schatz, J Craft, S Koby, M DeBaun, MR AF Schatz, J Craft, S Koby, M DeBaun, MR TI Asymmetries in visual-spatial processing following childhood stroke SO NEUROPSYCHOLOGY LA English DT Article ID SICKLE-CELL-DISEASE; HEMISPHERIC-SPECIALIZATION; BRAIN INJURY; HIERARCHICAL STIMULI; CHILDREN; ATTENTION; LESIONS; LATERALIZATION; REPRESENTATIONS AB The authors compared hemisphere-based and cognitive-domain-based hypotheses for visual-spatial deficits in children with stroke (n = 33) and children without stroke (n 43). Children with unilateral left (n = 14) or right (n = 7) injury showed less efficient search for the visual field contralateral to their injury. Right-hemisphere injury was associated with deficient global-level processing and coordinate spatial judgments. Left-hemisphere injury resulted in relatively intact local versus global processing and categorical versus coordinate judgments. Bilateral injury (n = 12) resulted in disruption of visual search across visual fields and relative deficits in global-level processing and coordinate spatial judgments. Recovery of visual-spatial processing in children following childhood stroke is task specific. Certain visual-spatial functions typically mediated by the left hemisphere appear less vulnerable to disruption. C1 Univ S Carolina, Dept Psychol, Columbia, SC 29208 USA. Seattle Amer Lake Vet Affairs Med Ctr, Ctr Geriatr Res Educ & Clin, Seattle, WA USA. Univ Washington, Dept Psychiat & Behav Sci, Seattle, WA 98195 USA. NIH, Dept Diagnost Radiol, Seattle, WA USA. Univ Washington, Sch Med, Dept Pediat, Seattle, WA 98195 USA. RP Schatz, J (reprint author), Univ S Carolina, Dept Psychol, Columbia, SC 29208 USA. EM schatz@sc.edu NR 42 TC 19 Z9 19 U1 0 U2 7 PU AMER PSYCHOLOGICAL ASSOC PI WASHINGTON PA 750 FIRST ST NE, WASHINGTON, DC 20002-4242 USA SN 0894-4105 J9 NEUROPSYCHOLOGY JI Neuropsychology PD APR PY 2004 VL 18 IS 2 BP 340 EP 352 DI 10.1037/0894-4105.18.2.340 PG 13 WC Psychology, Clinical; Neurosciences; Psychology SC Psychology; Neurosciences & Neurology GA 810SX UT WOS:000220725100015 PM 15099156 ER PT J AU Torregrossa, MM Isgor, C Folk, JE Rice, KC Watson, SJ Woods, JH AF Torregrossa, MM Isgor, C Folk, JE Rice, KC Watson, SJ Woods, JH TI The delta-opioid receptor agonist (+)BW373U86 regulates BDNF mRNA expression in rats SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE delta-opioid receptor; BDNF; TrkB; in situ hybridization; antidepressant; (+)BW373U86 ID DEPENDENT BEHAVIORAL STIMULATION; INCREASES HYPOXIC TOLERANCE; NEUROTROPHIC FACTOR; SYNAPTIC PLASTICITY; PARKINSONS-DISEASE; PIRIFORM CORTEX; NERVOUS-SYSTEM; MOOD DISORDERS; BRAIN; DEPRESSION AB delta-Opioid receptor agonists have antidepressant-like effects in behavioral models of depression. Chronic administration of classical antidepressants upregulates mRNA expression of brain-derived neurotrophic factor ( BDNF) and its high-affinity tyrosine kinase receptor, TrkB in the frontal cortex and hippocampus of rats. Increases in BDNF and TrkB levels are thought to be important for the therapeutic effects of these drugs. Therefore, we examined the ability of the delta-opioid receptor agonist (+) BW373U86 to regulate BDNF and TrkB mRNA expression in frontal cortex, hippocampus, as well as, basolateral amygdala, endopiriform nucleus, and primary olfactory cortex. At 3 h after a single administration of (+) BW373U86 animals were killed and BDNF and TrkB mRNA levels were examined by in situ hybridization. BDNF mRNA levels produced by (+) BW373U86 were compared to acute administration of the antidepressants desipramine and bupropion. A behaviorally antidepressant dose of 10 mg/kg (+) BW373U86 increased BDNF mRNA expression in all regions examined; a smaller dose of (+) BW373U86 (1 mg/kg) significantly increased BDNF mRNA expression only in frontal cortex. The delta-opioid receptor antagonist naltrindole blocked (+) BW373U86-mediated increases in BDNF mRNA expression. In addition, tolerance developed to increased BDNF mRNA expression with repeated injection, except in frontal cortex. Midazolam was administered to some animals to prevent the convulsions produced by (+) BW373U86, but midazolam did not block delta-opioid receptor-mediated increases in BDNF mRNA expression in frontal cortex, hippocampus, or amygdala. Unlike desipramine and bupropion, (+) BW373U86 upregulated BDNF mRNA expression acutely (within 3 h after a single administration). These data support the concept that delta-opioid receptor agonists may have antidepressant potential, and could be good targets for the development of faster-acting antidepressants. C1 Univ Michigan, Sch Med, Dept Pharmacol, Ann Arbor, MI 48109 USA. Univ Michigan, Neurosci Doctoral Program, Ann Arbor, MI 48109 USA. Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA. NIDDK, NIH, US Dept HHS, Bethesda, MD USA. RP Woods, JH (reprint author), Univ Michigan, Sch Med, Dept Pharmacol, 130 MSRB 3, Ann Arbor, MI 48109 USA. EM jhwoods@umich.edu FU NIDA NIH HHS [DA00254, DA13386, DA07281]; NIMH NIH HHS [MH42251] NR 38 TC 42 Z9 44 U1 0 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD APR PY 2004 VL 29 IS 4 BP 649 EP 659 DI 10.1038/sj.npp.1300345 PG 11 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 804ZI UT WOS:000220336200002 PM 14647482 ER PT J AU Munzar, P Tanda, G Justinova, Z Goldberg, SR AF Munzar, P Tanda, G Justinova, Z Goldberg, SR TI Histamine H3 receptor antagonists potentiate methamphetamine self-administration and methamphetamine-induced accumbal dopamine release SO NEUROPSYCHOPHARMACOLOGY LA English DT Article DE methamphetamine; histamine; clobenpropit; thioperamide; self-administration; dopamine-microdialysis ID H-3 RECEPTOR; TRANSPORTER FUNCTION; NUCLEUS-ACCUMBENS; RAT-BRAIN; ADENOSINERGIC MODULATION; REWARD PATHWAYS; INVOLVEMENT; STRIATUM; THIOPERAMIDE; MOUSE AB Methamphetamine administration increases brain levels of histamine and neuronal histamine attenuates several of methamphetamine's behavioral effects. The role of different subtypes of histamine receptors in this negative feedback, however, remains unclear. There is some evidence on possible involvement of histamine H3 receptors in these actions of methamphetamine. The aim of the present study was to evaluate the effects of two histamine H3 receptor antagonists, clobenpropit and thioperamide, on rewarding and neurochemical effects of methamphetamine utilizing three in vivo methodologies, drug self-administration, drug discrimination, and microdialysis in Sprague-Dawley rats. In rats self-administering methamphetamine intravenously under a fixed-ratio schedule, presession treatment with thioperamide (1.0-3.0 mg/kg, subcutaneous, s.c.) or clobenpropit (1.0-3.0 mg/kg, s.c.) potentiated the reinforcing effects of methamphetamine, as indicated by a dose-dependent increase in responding for a low 0.03 mg/kg dose of methamphetamine, that by itself failed to maintain responding above saline substitution levels, and a decrease in responding for a higher 0.06 mg/kg training dose of methamphetamine. In contrast, neither thioperamide nor clobenpropit treatment increased responding during saline substitution. In other rats trained to discriminate intraperitoneal (i.p.) injection of 1.0 mg/kg methamphetamine from i.p. injection of saline, both thioperamide and clobenpropit (0.3-3.0 mg/kg, s.c.) dose dependently increased methamphetamine-appropriate responding when administered with a low 0.3 mg/kg i.p. dose of methamphetamine, which by itself produced predominantly saline-appropriate responding. However, thioperamide and clobenpropit produced only saline-appropriate responding when administered with saline vehicle. Finally, thioperamide and clobenpropit potentiated methamphetamine-induced elevations in extracellular dopamine levels in the shell of the nucleus accumbens, but did not increase brain dopamine levels when given alone. These findings point to histamine H3 receptors as a new and important receptor system modulating the reinforcing, subjective, and neurochemical actions of methamphetamine. C1 NIDA, Preclin Pharmacol Sect, Behav Neurosci Res Branch,Intramural Res Program, NIH,US Dept HHS, Baltimore, MD 21224 USA. NIDA, Psychobiol Sect, Medicat Discovery Res Branch, Intramural Res Program,NIH,US Dept HHS, Baltimore, MD 21224 USA. RP Goldberg, SR (reprint author), NIDA, Preclin Pharmacol Sect, Behav Neurosci Res Branch,Intramural Res Program, NIH,US Dept HHS, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM sgoldber@intra.nida.nih.gov RI Tanda, Gianluigi/B-3318-2009; Justinova, Zuzana/A-9109-2011 OI Tanda, Gianluigi/0000-0001-9526-9878; Justinova, Zuzana/0000-0001-5793-7484 NR 57 TC 55 Z9 55 U1 0 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0893-133X J9 NEUROPSYCHOPHARMACOL JI Neuropsychopharmacology PD APR PY 2004 VL 29 IS 4 BP 705 EP 717 DI 10.1038/sj.npp.1300380 PG 13 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 804ZI UT WOS:000220336200008 PM 14735131 ER PT J AU Rosenberg, SA AF Rosenberg, SA TI Shedding light on immunotherapy for cancer SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Editorial Material ID T-CELLS; AUTOIMMUNITY; REGRESSION C1 NCI, Bethesda, MD 20892 USA. RP Rosenberg, SA (reprint author), NCI, Bethesda, MD 20892 USA. FU Intramural NIH HHS [Z01 SC003811-32] NR 5 TC 120 Z9 125 U1 0 U2 1 PU MASSACHUSETTS MEDICAL SOC/NEJM PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD APR 1 PY 2004 VL 350 IS 14 BP 1461 EP 1463 DI 10.1056/NEJMcibr045001 PG 3 WC Medicine, General & Internal SC General & Internal Medicine GA 808IJ UT WOS:000220562500016 PM 15070799 ER PT J AU Radzius, A Gallo, JJ Gorelick, DA Cadet, JL Uhl, G Henningfield, JE Moolchan, ET AF Radzius, A Gallo, JJ Gorelick, DA Cadet, JL Uhl, G Henningfield, JE Moolchan, ET TI Nicotine dependence criteria of the DIS and DSM-III-R: A factor analysis SO NICOTINE & TOBACCO RESEARCH LA English DT Article ID TOBACCO DEPENDENCE; FAGERSTROM TEST; SMOKING; CLASSIFICATION; WITHDRAWAL; PREVALENCE; TOLERANCE AB This paper reports a factor analysis of the symptoms of nicotine dependence that were determined in an assessment of 821 current cigarette-smoking research volunteers, according to the Diagnostic and Statistical Manual of Mental Disorders, 3rd edition, revised (DSM-III-R) of the American Psychiatric Association as well as an analysis of a subset who unsuccessfully attempted to quit (n=636). In the total sample, two factors with eigenvalues greater than 1 accounted for 62.7% of the variance. When the factor analysis was repeated with the subset of research volunteers who unsuccessfully attempted to quit, only one DSM-III-R nicotine dependence symptom loaded on the second factor. This finding suggests that the two-factor structure found in this and a previous factor analysis study of the nicotine dependence segment of the DSM-III-R may be an artifact of the skipout pattern of the DSM-III-R, which assumes that smokers who have not attempted to quit have not experienced withdrawal symptoms or used tobacco to avoid these symptoms. Goodness-of-fit measures suggested that the two-factor structure is a better fit than the one-factor structure for both the total population and the subset who unsuccessfully attempted to quit or cut down. Our sample of current smokers who had not attempted to quit (n = 185) was too small to permit factor analyses. Further work with other large samples from the general population of current smokers who have unsuccessfully attempted to quit as well as those who have not attempted to quit will enhance our understanding of the factor structure of the nicotine dependence segment of the DSM-III-R and clarify the effect of the skipout pattern on its factor structure. C1 Natl Inst Drug Abuse, NIH, Intramural Res Program, Baltimore, MD USA. Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mental Hyg, Baltimore, MD USA. Univ Penn, Dept Family Practice & Community Med, Philadelphia, PA 19104 USA. Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA. Pinney Associates, Bethesda, MD USA. RP Radzius, A (reprint author), POB 22696, Baltimore, MD 21203 USA. EM a.n.radziai@erols.com NR 20 TC 7 Z9 7 U1 2 U2 3 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 1462-2203 J9 NICOTINE TOB RES JI Nicotine Tob. Res. PD APR PY 2004 VL 6 IS 2 BP 303 EP 308 DI 10.1080/14622200410001676341 PG 6 WC Substance Abuse; Public, Environmental & Occupational Health SC Substance Abuse; Public, Environmental & Occupational Health GA 822AJ UT WOS:000221505400013 PM 15203804 ER PT J AU Weinberger, DR AF Weinberger, DR TI Genetic mechanisms of susceptibility to schizophrenia SO NORDIC JOURNAL OF PSYCHIATRY LA English DT Meeting Abstract C1 NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU TAYLOR & FRANCIS AS PI OSLO PA CORT ADELERSGT 17, PO BOX 2562, SOLLI, 0202 OSLO, NORWAY SN 0803-9488 J9 NORD J PSYCHIAT JI Nord. J. Psychiatr. PD APR PY 2004 VL 58 IS 2 BP 88 EP 88 PG 1 WC Psychiatry SC Psychiatry GA 810FP UT WOS:000220690500020 ER PT J AU Wesley, JN McGee, EC Garmestani, K Brechbiel, MW Yordanov, AT Wu, C Gansow, OA Eckelman, WC Bacher, JD Flynn, M Goldman, CK MacLin, M Schwartz, UP Jackson-White, T Phillip, CM Decker, J Waldmann, TA AF Wesley, JN McGee, EC Garmestani, K Brechbiel, MW Yordanov, AT Wu, C Gansow, OA Eckelman, WC Bacher, JD Flynn, M Goldman, CK MacLin, M Schwartz, UP Jackson-White, T Phillip, CM Decker, J Waldmann, TA TI Systemic radioimmunotherapy using a monoclonal antibody, anti-Tac directed toward the alpha subunit of the IL-2 receptor armed with the alpha-emitting radionuclides Bi-212 or At-211 SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article DE alpha-particle; astatine; At-211; bismuth; Bi-212; monoclonal antibody; radioimmunotherapy of in vivo animal models ID T-CELL LEUKEMIA; INTERLEUKIN-2 RECEPTOR; TARGETED THERAPY; TRANSPLANTATION; PRIMATE; IMMUNOTHERAPY; STABILITY; LYMPHOMA; BLOCKADE; AT-211 AB To exploit the fact that IL-2 receptors are expressed by T-cells responding to foreign antigens but not by resting T-cells, humanized anti-Tac (HAT) armed with alpha-emitting radionuclides Bi-212 and At-211 was evaluated in a cynomolgus cardiac allograft model. Control graft survival was 8.2 +/- 0.5 days compared with 14.0 +/- 1.3 days (p<0.01) survival for monkeys treated with Bi-212 labeled HAT and 26.7 +/- 2.4 days survival (p < 0.001 versus controls) with At-211 labeled HAT. Thus, At-211 labeled HAT may have application in organ transplantation and in treatment of IL-2 receptor expressing T-cell leukemia. Published by Elsevier Inc. C1 NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. NCI, Radiat Oncol Branch, NIH, Bethesda, MD 20892 USA. NIH, Dept Nucl Med, Ctr Clin, Bethesda, MD USA. NIH, Vet Resources Program, Off Res Serv, Bethesda, MD USA. NHLBI, NIH, Bethesda, MD 20892 USA. RP Waldmann, TA (reprint author), NCI, Metab Branch, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. EM tawald@helix.nih.gov NR 30 TC 5 Z9 8 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0969-8051 J9 NUCL MED BIOL JI Nucl. Med. Biol. PD APR PY 2004 VL 31 IS 3 BP 357 EP 364 DI 10.1016/j.nucmedbio.2003.08.011 PG 8 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 804TK UT WOS:000220320800007 PM 15028248 ER PT J AU Zhang, HL Meng, LH Zimonjic, DB Popescu, NC Pommier, Y AF Zhang, HL Meng, LH Zimonjic, DB Popescu, NC Pommier, Y TI Thirteen-exon-motif signature for vertebrate nuclear and mitochondrial type IB topoisomerases SO NUCLEIC ACIDS RESEARCH LA English DT Article ID DNA TOPOISOMERASES AB DNA topoisomerases contribute to various cellular activities that involve DNA. We previously identified a human nuclear gene that encodes a mitochondrial DNA topoisomerase. Here we show that genes for mitochondrial DNA topoisomerases (type IB) exist only in vertebrates. A 13-exon topoisomerase motif was identified as a characteristic of genes for both nuclear and mitochondrial type IB topoisomerases. The presence of this signature motif is thus an indicator of the coexistence of nuclear and mitochondrial type IB DNA topoisomerases. We hypothesize that the prototype topoisomerase IB with the 13-exon structure formed first, and then duplicated. One topoisomerase specialized for nuclear DNA and the other for mitochondrial DNA. C1 NCI, Mol Pharmacol Lab, NIH, DHHS, Bethesda, MD 20892 USA. NCI, Expt Carcinogenesis Lab, Ctr Canc Res, NIH,DHHS, Bethesda, MD 20892 USA. RP Pommier, Y (reprint author), NCI, Mol Pharmacol Lab, NIH, DHHS, Bethesda, MD 20892 USA. EM pommier@nih.gov NR 9 TC 23 Z9 26 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 7 BP 2087 EP 2092 DI 10.1093/nar/gkh525 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 816YO UT WOS:000221145400004 PM 15096574 ER PT J AU Cui, S Arosio, D Doherty, KM Brosh, RM Falaschi, A Vindigni, A AF Cui, S Arosio, D Doherty, KM Brosh, RM Falaschi, A Vindigni, A TI Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A SO NUCLEIC ACIDS RESEARCH LA English DT Article ID WERNER-SYNDROME PROTEIN; SYNDROME GENE-PRODUCT; DNA-BINDING PROTEIN; BLOOMS-SYNDROME HELICASE; ESCHERICHIA-COLI; BIOCHEMICAL-CHARACTERIZATION; FUNCTIONAL INTERACTION; MOLECULAR-CLONING; SUBSTRATE-SPECIFICITY; BLM HELICASE AB RecQ helicases are required for the maintenance of genome stability. Characterization of the substrate specificity and identification of the binding partners of the five human RecQ helicases are essential for understanding their function. In the present study, we have developed an efficient baculovirus expression system that allows us to obtain milligram quantities of recombinant RECQ1. Our gel filtration and dynamic light scattering experiments show that RECQ1 has an apparent molecular mass of 158 kDa and a hydrodynamic radius of 5.4 +/- 0.6 nm, suggesting that RECQ1 forms dimers in solution. The oligomeric state of RECQ1 remains unchanged upon binding to a single-stranded (ss)DNA fragment of 50 nt. We show that RECQ1 alone is able to unwind short DNA duplexes (<110 bp), whereas considerably longer substrates (501 bp) can be unwound only in the presence of human replication protein A (hRPA). The same experiments with Escherichia coli SSB show that RECQ1 is specifically stimulated by hRPA. However, hRPA does not affect the ssDNA-dependent ATPase activity of RECQ1. In addition, our far western, ELISA and co-immunoprecipitation experiments demonstrate that RECQ1 physically interacts with the 70 kDa subunit of hRPA and that this interaction is not mediated by DNA. C1 Int Ctr Genet Engn & Biotechnol, I-34012 Trieste, Italy. NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. RP Vindigni, A (reprint author), Int Ctr Genet Engn & Biotechnol, Padriciano 99, I-34012 Trieste, Italy. EM vindigni@icgeb.org RI arosio, daniele/B-3160-2012; CUI, Sheng/M-6405-2013 OI CUI, Sheng/0000-0001-6329-3582 NR 70 TC 77 Z9 78 U1 0 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 7 BP 2158 EP 2170 DI 10.1093/nar/gkh540 PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 816YO UT WOS:000221145400012 PM 15096578 ER PT J AU Stuart, JA Hashiguchi, K Wilson, DM Copeland, WC Souza-Pinto, NC Bohr, VA AF Stuart, JA Hashiguchi, K Wilson, DM Copeland, WC Souza-Pinto, NC Bohr, VA TI DNA base excision repair activities and pathway function in mitochondrial and cellular lysates from cells lacking mitochondrial DNA SO NUCLEIC ACIDS RESEARCH LA English DT Article ID POLYMERASE-GAMMA; EXPRESSION; NUCLEAR; MTDNA; COMPLEMENTATION; IDENTIFICATION; REPLICATION; MODULATION; ACTIVATION; PROMOTER AB Mitochondrial DNA (mtDNA) contains higher steady-state levels of oxidative damage and mutates at rates significantly greater than nuclear DNA. Oxidative lesions in mtDNA are removed by a base excision repair (BER) pathway. All mtDNA repair proteins are nuclear encoded and imported. Most mtDNA repair proteins so far discovered are either identical to nuclear DNA repair proteins or isoforms of nuclear proteins arising from differential splicing. Regulation of mitochondrial BER is therefore not expected to be independent of nuclear BER, though the extent to which mitochondrial BER is regulated with respect to mtDNA amount or damage is largely unknown. Here we have measured DNA BER activities in lysates of mitochondria isolated from human 143B TK- osteosarcoma cells that had been depleted of mtDNA (rho(0)) or not (wt). Despite the total absence of mtDNA in the rho(0) cells, a complete mitochondrial BER pathway was present, as demonstrated using an in vitro assay with synthetic oligonucleotides. Measurement of individual BER protein activities in mitochondrial lysates indicated that some BER activities are insensitive to the lack of mtDNA. Uracil and 8-oxoguanine DNA glycosylase activities were relatively insensitive to the absence of mtDNA, only about 25% reduced in rho(0) relative to wt cells. Apurinic/apyrimidinic (AP) endonuclease and polymerase gamma activities were more affected, 65 and 45% lower, respectively, in rho(0) mitochondria. Overall BER activity in lysates was also about 65% reduced in rho(0) mitochondria. To identify the limiting deficiencies in BER of rho(0) mitochondria we supplemented the BER assay of mitochondrial lysates with pure uracil DNA glycosylase, AP endonuclease and/or the catalytic subunit of polymerase gamma. BER activity was stimulated by addition of uracil DNA glycosylase and polymerase gamma. However, no addition or combination of additions stimulated BER activity to wt levels. This suggests that an unknown activity, factor or interaction important in BER is deficient in rho(0) mitochondria. While nuclear BER protein levels and activities were generally not altered in rho(0) cells, AP endonuclease activity was substantially reduced in nuclear and in whole cell extracts. This appeared to be due to reduced endogenous reactive oxygen species (ROS) production in rho(0) cells, and not a general dysfunction of rho(0) cells, as exposure of cells to ROS rapidly stimulated increases in AP endonuclease activities and APE1 protein levels. C1 NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA. NIEHS, Genet Mol Lab, Res Triangle Pk, NC 27709 USA. RP Bohr, VA (reprint author), 5600 Nathan Shock Dr,Box 1, Baltimore, MD 21224 USA. EM bohrv@grc.nia.nih.gov RI Souza-Pinto, Nadja/C-3462-2013 OI Souza-Pinto, Nadja/0000-0003-4206-964X NR 31 TC 37 Z9 39 U1 0 U2 1 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 7 BP 2181 EP 2192 DI 10.1093/nar/gkh533 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 816YO UT WOS:000221145400014 PM 15107486 ER PT J AU Fan, JS Otterlei, M Wong, HK Tomkinson, AE Wilson, DM AF Fan, JS Otterlei, M Wong, HK Tomkinson, AE Wilson, DM TI XRCC1 co-localizes and physically interacts with PCNA SO NUCLEIC ACIDS RESEARCH LA English DT Article ID STRAND-BREAK REPAIR; CELL NUCLEAR ANTIGEN; BASE EXCISION-REPAIR; OXIDATIVE DNA-DAMAGE; SISTER-CHROMATID EXCHANGE; POLY(ADP-RIBOSE) POLYMERASE; LIGASE-III; HOMOLOGOUS RECOMBINATION; PROTEIN XRCC1; MAJOR HUMAN AB X-ray Repair Cross Complementing 1 (XRCC1) is thought to function as a scaffolding protein in both base excision repair and single-strand break repair (SSBR), since it interacts with several proteins participating in these related pathways and has no known enzymatic activity. Moreover, studies indicate that XRCC1 possesses discrete G(1) and S phase-specific functions. To further define the contribution of XRCC1 to DNA metabolism, we determined the in vivo localization pattern of this protein and searched for novel protein interactors. We report here that XRCC1 co-localizes with proliferating cell nuclear antigen (PCNA) at DNA replication foci, observed exclusively in the S phase of undamaged HeLa cells. Furthermore, fluorescence resonance energy transfer (FRET) analysis and co-immunoprecipitation indicate that XRCC1 and PCNA are in a complex and likely physically interact in vivo. In vitro biochemical analysis demonstrated that these two proteins associate directly, with the interaction being mediated by residues between amino acids 166 and 310 of XRCC1. The current evidence suggests a model where XRCC1 is sequestered via its interaction with PCNA to sites of DNA replication factories to facilitate efficient SSBR in S phase. C1 NIA, Lab Mol Gerontol, Baltimore, MD 21224 USA. Norwegian Univ Sci & Technol, Dept Canc Res & Mol Med, N-7489 Trondheim, Norway. Univ Maryland, Med Ctr, Dept Radiat Oncol, Radiat Oncol Res Lab, Baltimore, MD 21201 USA. Univ Maryland, Med Ctr, Greenbaum Canc Ctr, Baltimore, MD 21201 USA. RP Wilson, DM (reprint author), NIA, Lab Mol Gerontol, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. EM wilsonda@grc.nia.nih.gov FU NIEHS NIH HHS [ES012521]; NIGMS NIH HHS [GM57479, R01 GM057479] NR 53 TC 131 Z9 141 U1 0 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 7 BP 2193 EP 2201 DI 10.1093/nar/gkh556 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 816YO UT WOS:000221145400015 PM 15107487 ER PT J AU Kolker, E Makarova, KS Shabalina, S Picone, AF Purvine, S Holzman, T Cherny, T Armbruster, D Munson, RS Kolesov, G Frishman, D Galperin, MY AF Kolker, E Makarova, KS Shabalina, S Picone, AF Purvine, S Holzman, T Cherny, T Armbruster, D Munson, RS Kolesov, G Frishman, D Galperin, MY TI Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae SO NUCLEIC ACIDS RESEARCH LA English DT Article ID GENOME-SCALE ANALYSIS; STRAIN RD KW20; ESCHERICHIA-COLI; CRYSTAL-STRUCTURE; INTERACTING PROTEINS; STRUCTURAL GENOMICS; STATISTICAL-MODEL; DATABASE SEARCH; PSI-BLAST; SEQUENCE AB The progress in genome sequencing has led to a rapid accumulation in GenBank submissions of uncharacterized 'hypothetical' genes. These genes, which have not been experimentally characterized and whose functions cannot be deduced from simple sequence comparisons alone, now comprise a significant fraction of the public databases. Expression analyses of Haemophilus influenzae cells using a combination of transcriptomic and proteomic approaches resulted in confident identification of 54 'hypothetical' genes that were expressed in cells under normal growth conditions. In an attempt to understand the functions of these proteins, we used a variety of publicly available analysis tools. Close homologs in other species were detected for each of the 54 'hypothetical' genes. For 16 of them, exact functional assignments could be found in one or more public databases. Additionally, we were able to suggest general functional characterization for 27 more genes (comprising similar to80% total). Findings from this analysis include the identification of a pyruvate-formate lyase-like operon, likely to be expressed not only in H.influenzae but also in several other bacteria. Further, we also observed three genes that are likely to participate in the transport and/or metabolism of sialic acid, an important component of the H.influenzae lipo-oligosaccharide. Accurate functional annotation of uncharacterized genes calls for an integrative approach, combining expression studies with extensive computational analysis and curation, followed by eventual experimental verification of the computational predictions. C1 BIATECH, Bothell, WA 98011 USA. Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. Ohio State Univ, Columbus, OH 43205 USA. Childrens Res Inst, Columbus, OH 43205 USA. Tech Univ Munich, Wissenshaftzentrum Weihenstephan, D-85354 Freising Weihenstephan, Germany. RP Kolker, E (reprint author), BIATECH, 19310 N Creek Pkwy,Suite 115, Bothell, WA 98011 USA. EM ekolker@biatech.org RI Kolker, Eugene/C-6711-2008; Munson, Jr, Robert/E-3710-2011; Galperin, Michael/B-5859-2013; Shabalina, Svetlana/N-8939-2013 OI Munson, Jr, Robert/0000-0002-3204-3019; Galperin, Michael/0000-0002-2265-5572; Shabalina, Svetlana/0000-0003-2272-7473 FU Intramural NIH HHS [Z99 LM999999]; NIDCD NIH HHS [R01 DC005980, R01 DC003915, R01 DC03915, R55 DC003915] NR 61 TC 53 Z9 58 U1 0 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 8 BP 2353 EP 2361 DI 10.1093/nar/gkh555 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 820RE UT WOS:000221406200008 PM 15121896 ER PT J AU Jin, JS Baek, S Lee, H Oh, MY Koo, YE Shim, MS Kwon, SY Jeon, I Park, SY Baek, K Yoo, MA Hatfield, DL Lee, BJ AF Jin, JS Baek, S Lee, H Oh, MY Koo, YE Shim, MS Kwon, SY Jeon, I Park, SY Baek, K Yoo, MA Hatfield, DL Lee, BJ TI A DNA replication-related element downstream from the initiation site of Drosophila selenophosphate synthetase 2 gene is essential for its transcription SO NUCLEIC ACIDS RESEARCH LA English DT Article ID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE; FACTOR DREF; PROMOTER; EXPRESSION; MELANOGASTER; CELLS; SELENOCYSTEINE; SEQUENCE; SYSTEM; RNA AB Selenophosphate synthetase catalyzes the synthesis of selenophosphate which is a selenium donor for Sec biosynthesis. In Drosophila melanogaster, there are two types of selenophosphate synthetases designated dSPS1 and dSPS2, where dSPS2 is a selenoprotein. The mechanism of gene expression of dSPS2 as well as other selenoproteins in Drosophila has not been elucidated. Herein, we report an essential regulator system that regulates the transcription of the dSPS2 gene (dsps2). Through deletion/substitution mutagenesis, the downstream DNA replication-related element (DRE) located at +71 has been identified as an essential element for dsps2 promoter activity. Furthermore, double-stranded RNA interference (dsRNAi) experiments were performed to ablate transcription factors such as TBP, TRF1, TRF2 and DREF in Schneider cells. The dsRNAi experiments showed that dsps2 promoter activities in DREF- and TRF2-depleted cells were significantly decreased by 90% and 50%, respectively. However, the depletion of TBP or TRF1 did not affect the expression level of dsps2 even though there is a putative TATA box at -20. These results strongly suggest that the DRE/DREF system controls the basal level of transcription of dsps2 by interacting with TRF2. C1 Seoul Natl Univ, Sch Biol Sci, Seoul 151, South Korea. Seoul Natl Univ, Inst Mol Biol & Genet, Seoul 151, South Korea. Kyung Hee Univ, Grad Sch Biotechnol, Yongin, South Korea. Pusan Natl Univ, Dept Mol Biol, Pusan, South Korea. NCI, Mol Biol Selenium Sect, Lab Canc Prevent, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. RP Lee, BJ (reprint author), Seoul Natl Univ, Sch Biol Sci, Seoul 151, South Korea. EM imbglmg@plaza.snu.ac.kr RI Kwon, So Yeon/M-5625-2014 OI Kwon, So Yeon/0000-0002-8490-9101 NR 32 TC 10 Z9 11 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 8 BP 2482 EP 2493 DI 10.1093/nar/gkh569 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 820RE UT WOS:000221406200021 PM 15121905 ER PT J AU Hawkins, ME Balis, FM AF Hawkins, ME Balis, FM TI Use of pteridine nucleoside analogs as hybridization probes SO NUCLEIC ACIDS RESEARCH LA English DT Article ID CONTAINING DEOXYTRIDECANUCLEOTIDE DUPLEXES; CONFORMATIONAL TRANSITIONS; FLUORESCENCE PROPERTIES; MOLECULAR BEACONS; GUANOSINE ANALOG; HIV-1 INTEGRASE; DNA; OLIGONUCLEOTIDES; TEMPERATURE; KINETICS AB The pteridine nucleoside analog 3-methyl isoxanthopterin (3-MI) is highly fluorescent, with a quantum yield of 0.88, and it can be synthesized as a phosphoramidite and incorporated into oligonucleotides through a deoxyribose linkage. Within an oligonucleotide, 3-MI is intimately associated with native bases and its fluorescence is variably quenched in a sequence-dependent manner. Bend ing, annealing, binding, digestion or cleavage of fluorophore-containing oligonucleotides can be detected by monitoring changes in fluorescence properties. We developed a single step method for detecting annealing of complementary DNA sequences using 3-MI-containing oligonucleotides as hybridization probes. One of the complementary strands contains the fluorophore as an insertion and when annealing occurs, the fluorophore bulges out from the double strand, resulting in increased fluorescence intensity. We have examined the sequence dependency, optimal strand length and impact of multiple fluorophores per strand in terms of brightness and impact on the annealing process. We describe the application of this technique to the detection of positive PCR products using an HIV-1 detection system. This sequence-dependent hybridization technique can result in fluorescence intensity increases of up to 27-fold. Fluorescence intensity increases are only seen upon specific binding to bulge-generating complements, removing issues of high background from non-specific binding. C1 NCI, Pediat Oncol Branch, NIH, Bethesda, MD 20892 USA. RP Hawkins, ME (reprint author), NCI, Pediat Oncol Branch, NIH, 10-13C116,10 Ctr Dr, Bethesda, MD 20892 USA. EM mh100x@nih.gov NR 20 TC 22 Z9 22 U1 0 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 7 AR e62 DI 10.1093/nar/gnh060 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 816YO UT WOS:000221145400028 PM 15090623 ER PT J AU Karadag, A Riminucci, M Bianco, P Cherman, N Kuznetsov, SA Nguyen, N Collins, MT Robey, PG Fisher, LW AF Karadag, A Riminucci, M Bianco, P Cherman, N Kuznetsov, SA Nguyen, N Collins, MT Robey, PG Fisher, LW TI A novel technique based on a PNA hybridization probe and FRET principle for quantification of mutant genotype in fibrous dysplasia/McCune-Albright syndrome SO NUCLEIC ACIDS RESEARCH LA English DT Article ID POLYMERASE-CHAIN-REACTION; MITOCHONDRIAL-DNA; POINT MUTATIONS; DIABETES-MELLITUS; GENE; BONE; DISEASE; ACCUMULATION; HETEROPLASMY; CARDIOMYOPATHY AB Somatic mutations are present in various proportions in numerous developmental pathologies. Somatic activating missense mutations of the GNAS gene encoding the Gs(alpha) protein have previously been shown to be the cause of fibrous dysplasia of bone (FD)/McCune-Albright syndrome (MAS). Because in MAS patients, tissues as diverse as melanocytes, gonads and bone are affected, it is generally accepted that the GNAS mutation in this disease must have occurred early in development. Interestingly, it has been shown that the development of an active FD lesion may require both normal and mutant cells. Studies of the somatic mosaic states of FD/MAS and many other somatic diseases need an accurate method to determine the ratio of mutant to normal cells in a given tissue. A new method for quantification of the mutant:normal ratio of cells using a PNA hybridization probe-based FRET technique was developed. This novel technique, with a linear sensitivity of 2.5% mutant alleles, was used to detect the percentage mutant cells in a number of tissue and cell culture samples derived from FD/MAS lesions and could easily be adapted for the quantification of mutations in a large spectrum of diseases including cancer. C1 Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, DHHS, Bethesda, MD 20892 USA. Univ Aquila, Dipartimento Med Sperimentale, I-67100 Laquila, Italy. Parco Sci Biomed San Raffaele, Rome, Italy. Univ Roma La Sapienza, Dipartimento Med Sperimentale & Patol, Rome, Italy. US FDA, Ctr Biol Evaluat & Res, Dept Hlth & Human Serv, Bethesda, MD USA. RP Karadag, A (reprint author), Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, DHHS, 30 Convent Dr,Bldg 30,Room 223,MSC 4320, Bethesda, MD 20892 USA. EM akaradag@dir.nidcr.nih.gov RI Robey, Pamela/H-1429-2011 OI Robey, Pamela/0000-0002-5316-5576 FU Telethon [E.1029] NR 40 TC 25 Z9 26 U1 1 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2004 VL 32 IS 7 AR e63 DI 10.1093/nar/gnh059 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 816YO UT WOS:000221145400029 PM 15096559 ER PT J AU Kohgo, S Yamada, K Kitano, K Iwai, Y Sakata, S Ashida, N Hayakawa, H Nameki, D Kodama, E Matsuoka, M Mitsuya, H Ohrui, H AF Kohgo, S Yamada, K Kitano, K Iwai, Y Sakata, S Ashida, N Hayakawa, H Nameki, D Kodama, E Matsuoka, M Mitsuya, H Ohrui, H TI Design, efficient synthesis, and anti-HIV activity of 4 '-C-cyano- and 4 '-C-ethynyl-2 '-deoxy purine nucleosides SO NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS LA English DT Article DE NRTIs; efficient synthesis; 4 '-CNdNs; 4 '-EdNs; anti-HIV-1 agents ID HUMAN-IMMUNODEFICIENCY-VIRUS; CELL-LINE; NUCLEOTIDES; RESISTANCE; VARIANTS; ANALOGS; TYPE-1; GENE AB Some 4'-C-ethynyl-2'-deoxy purine nucleosides showed the most potent anti-HIV activity among the series of 4'-C-substituted 2'-deoxynucleosides whose 4'-C-substituents were methyl, ethyl, ethynyl and so on. Our hypothesis is that the smaller the substituent at the C-4' position they have, the more acceptable biological activity they show. Thus, 4'-C-cyano-2'-deoxy purine nucleosides, whose substituent is smaller than the ethynyl group, will have more potent antiviral activity. To prove our hypothesis, we planned to develop an efficient synthesis of 4'-C-cyano-2'-deoxy purine nucleosides (4'-CNdNs) and 4'-C-ethynyl-2'-deoxy purine nucleosides (4'-EdNs). Consequently, we succeeded in developing an efficient synthesis of six 2'-deoxy purine nucleosides bearing either a cyano or an ethynyl group at the C-4' position of the sugar moiety from 2'-deoxyadenosine and 2,6-diaminopurine 2'-deoxyriboside. Unfortunately, 4'-C-cyano derivatives showed lower activity against HIV-1, and two 4'-C-ethynyl derivatives suggested high toxicity in vivo. C1 Yamasa Corp, Div Biochem, Chem & Pharmacol Lab, Choshi, Chiba 2880056, Japan. Tohoku Univ, Grad Sch Life Sci, Div Life Sci, Sendai, Miyagi 980, Japan. NCI, Expt Retrovirol Sect, Dept Dev Therapeut, Med Branch, Bethesda, MD 20892 USA. Kumamoto Univ, Sch Med, Dept Internal Med 2, Kumamoto 860, Japan. Kyoto Univ, Inst Virus Res, Lab Virus Immunol, Kyoto 606, Japan. RP Hayakawa, H (reprint author), Yamasa Corp, Div Biochem, Chem & Pharmacol Lab, 10-1 Araoicho 2 Chome, Choshi, Chiba 2880056, Japan. EM hayakawa@yamasa.com RI Kodama, Eiichi /C-4032-2009; OI Kodama, Eiichi /0000-0002-6622-2752; Matsuoka, Masao/0000-0002-0473-754X NR 19 TC 22 Z9 22 U1 1 U2 4 PU MARCEL DEKKER INC PI NEW YORK PA 270 MADISON AVE, NEW YORK, NY 10016 USA SN 1525-7770 J9 NUCLEOS NUCLEOT NUCL JI Nucleosides Nucleotides Nucleic Acids PD APR PY 2004 VL 23 IS 4 BP 671 EP 690 DI 10.1081/NCN-120037508 PG 20 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 826JI UT WOS:000221825000003 PM 15200030 ER PT J AU Whiteside, MA Heimburger, DC Johanning, GL AF Whiteside, MA Heimburger, DC Johanning, GL TI Micronutrients and cancer therapy SO NUTRITION REVIEWS LA English DT Review DE cisplatin; micronutrients; drug resistance ID MULTIPLE ANTIOXIDANT VITAMINS; FOLIC-ACID DEFICIENCY; CELL LUNG-CANCER; FOLATE STATUS; SERUM FOLATE; DIETARY ANTIOXIDANTS; BREAST-CANCER; IN-VIVO; CHEMOTHERAPY; TUMOR AB The effect of micronutrient supplementation on the effectiveness of cancer chemotherapeutic agents is reviewed, and the efficacy of antioxidants, folic acid, and other vitamins and minerals is discussed. Although some micronutrients show promise in enhancing the cytotoxicity of anticancer agents in vitro, caution should be exercised in recommending micronutrient supplementation for cancer patients receiving chemotherapeutic drugs. To date, few well-controlled clinical trials have been conducted to evaluate the efficacy of micronutrients in promoting the sensitivity of tumors to chemotherapeutic agents. C1 NCI, Canc Prevent Fellowship Program, Div Canc Prevent, Bethesda, MD 20892 USA. Univ Alabama, Dept Nutr Sci, Birmingham, AL 35294 USA. Univ Alabama, Dept Med, Birmingham, AL 35294 USA. Univ Texas, MD Anderson Canc Ctr, Dept Vet Sci, Bastrop, TX 78602 USA. RP Whiteside, MA (reprint author), NCI, Canc Prevent Fellowship Program, Div Canc Prevent, Bethesda, MD 20892 USA. NR 41 TC 10 Z9 10 U1 1 U2 2 PU INT LIFE SCIENCES INST NORTH AMERICA PI WASHINGTON PA ONE THOMAS CIRCLE, N W, 9TH FLOOR, WASHINGTON, DC 20005 USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD APR PY 2004 VL 62 IS 4 BP 142 EP 147 DI 10.1301/nr.2004.apr.142-147 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 903NY UT WOS:000227434000003 PM 15141429 ER PT J AU Costello, R Finkelstein, J Dell'Orto, M AF Costello, R Finkelstein, J Dell'Orto, M TI Executive summary: Conference on dietary supplement use in the elderly - Proceedings of the conference held January 14-15, 2003, Natcher Auditorium, National Institutes of Health, Bethesda, MD SO NUTRITION REVIEWS LA English DT Review ID RANDOMIZED CONTROLLED TRIAL; ACETYL-L-CARNITINE; VITAMIN-E SUPPLEMENTATION; HIGH-DOSE SUPPLEMENTATION; OXIDATIVE DNA-DAMAGE; ALPHA-LIPOIC ACID; BETA-CAROTENE; BREAST-CANCER; FATTY-ACIDS; OLD RATS AB "Dietary Supplement Use in the Elderly" was third in a series of conferences on dietary supplement use through the lifespan organized by the NIH. Serving as scientific chair of the conference was Robert Russell, M.D., Director and Senior Scientist of the Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University; as honorary chair was Nancy Wellman, Ph.D., FADA, Director of the National Policy and Resource Center on Nutrition and Aging, Florida International University. Invited speakers were leading scientists in the fields of nutrition, metabolism, and aging. Close to 300 researchers, government representatives, and public health professionals attended the conference. Conference materials, including PowerPoint presentations, are posted at the Office of Dietary Supplements website http://dietary-supplements,info.nih.gov/ showpage.aspx?pageid=148. In addition, a comprehensive bibliography of Dietary Supplement Use in the Elderly, prepared by the National Library of Medicine to complement the workshop materials, can be accessed through the library's website at http://www.nlm.nih.gov/pubs/ cbm/elderdietsuppl.html. C1 NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. NIA, Off Nutr, NIH, Bethesda, MD 20892 USA. RP Costello, R (reprint author), NIH, Off Dietary Supplements, 6100 Execut Blvd,Room 3B01,MSC 7517, Bethesda, MD 20892 USA. EM CostellB@od.nih.gov NR 80 TC 3 Z9 4 U1 3 U2 3 PU INT LIFE SCIENCES INST NORTH AMERICA PI WASHINGTON PA ONE THOMAS CIRCLE, N W, 9TH FLOOR, WASHINGTON, DC 20005 USA SN 0029-6643 J9 NUTR REV JI Nutr. Rev. PD APR PY 2004 VL 62 IS 4 BP 160 EP 175 DI 10.1301/nr.2004.apr.160-175 PG 16 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 903NY UT WOS:000227434000006 ER PT J AU Nguyen, RHN Keller, J Bunge, K Virgo, D Ross, BJ Anderson, JR AF Nguyen, RHN Keller, J Bunge, K Virgo, D Ross, BJ Anderson, JR TI Attributable risk of cocaine use on premature rupture of membranes among human immunodeficiency virus-infected women SO OBSTETRICS AND GYNECOLOGY LA English DT Meeting Abstract CT 52nd Annual Clinical Meeting of the American-College-of-Obstetricians-and-Gynecologists CY MAY 01-05, 2004 CL Philadelphia, PA SP Amer Coll Obstet & Gynecologists C1 NIEHS, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0029-7844 J9 OBSTET GYNECOL JI Obstet. Gynecol. PD APR PY 2004 VL 103 IS 4 SU S BP 10S EP 10S PG 1 WC Obstetrics & Gynecology SC Obstetrics & Gynecology GA 876BH UT WOS:000225470000021 ER PT J AU Thangaraju, M Sharan, S Sterneck, E AF Thangaraju, M Sharan, S Sterneck, E TI Comparison of mammary gland involution between 129S1 and C57BL/6 inbred mouse strains: differential regulation of Bcl2a1, Trp53, Cebpb, and Cebpd expression SO ONCOGENE LA English DT Article DE C/EBP; mouse strain; mammary gland; Involution; Bfl1; p53 ID PLASMACYTOMA SUSCEPTIBILITY; CELL-PROLIFERATION; EPITHELIAL-CELLS; GENE-EXPRESSION; BCL-2 HOMOLOG; BREAST-CANCER; EGF RECEPTOR; C/EBP-BETA; APOPTOSIS; FAMILY AB Genetic engineering has made the mouse an invaluable tool to address the function of individual genes in a targeted manner. Over the last decade it has become apparent that the genetic mouse strain background can significantly influence the phenotype of an engineered mouse. Therefore, it is essential to characterize the biology of the different wild-type background strains. In this study, we have compared mouse mammary gland involution in the 129S1 and C57BL/6 inbred strains and report significant differences at the molecular level with differential expression of Bcl2a1 (Bfl1), Trp53 (p53), Cebpb (C/EBPbeta), and Cebpd (C/EBPdelta). The C57BL/6 strain exhibits dynamic responses with induction of Trp53 and Cebpd and concomitant downregulation of Bcl2a1 during the first phase of involution. In contrast, expression of these genes does not change significantly in 129S1 mice. During the second phase, C57BL/6 glands contain more Cebpb than 129S1 glands. Nevertheless, involution proceeds morphologically with similar kinetics in both strains. The data demonstrate that the genetic response of mammary tissue varies significantly between 129S1 and C57BL/6. These results may provide a basis for the interpretation of strain-specific phenotypes in engineered mice and underline the importance of pure strains for large-scale expression studies with mutant mice. C1 NCI, Regulat Cell Growth Lab, Ctr Canc Res, NIH, Frederick, MD 21702 USA. RP Sterneck, E (reprint author), NCI, Regulat Cell Growth Lab, Ctr Canc Res, NIH, POB B, Frederick, MD 21702 USA. EM sterneck@ncifcrf.gov NR 32 TC 8 Z9 8 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0950-9232 J9 ONCOGENE JI Oncogene PD APR 1 PY 2004 VL 23 IS 14 BP 2548 EP 2553 DI 10.1038/sj.onc.1207363 PG 6 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA 808GQ UT WOS:000220558000012 PM 14981542 ER PT J AU Abrams, JS Enos, RA Schoenfeldt, M AF Abrams, JS Enos, RA Schoenfeldt, M TI Current phase III, NCI-supported, cooperative group clinical trials in breast cancer SO ONCOLOGY-NEW YORK LA English DT Editorial Material C1 NCI, Bethesda, MD 20892 USA. Emmes Corp, Rockville, MD USA. RP Abrams, JS (reprint author), NCI, Bethesda, MD 20892 USA. NR 2 TC 0 Z9 0 U1 0 U2 0 PU P R R INC PI MELVILLE PA 48 SOUTH SERVICE RD, MELVILLE, NY 11747 USA SN 0890-9091 J9 ONCOLOGY-NY JI Oncology-NY PD APR PY 2004 VL 18 IS 4 BP 463 EP + PG 5 WC Oncology SC Oncology GA 052DG UT WOS:000238210700012 PM 15134354 ER PT J AU Schmidt, P Ciner, E Cyert, L Dobson, V Kulp, MT Maguire, M Moore, B Orel-Bixler, D Peskin, E Quinn, G Redford, M Schultz, J Beck, R Cotter, S Holmes, J Shipp, M West, S Schmidt, P Maguire, M Dobson, V Quinn, G Ciner, E Cyert, L Kulp, MT Moore, B Orel-Bixler, D Redford, M Ying, GS Orel-Bixler, D Qualley, P Howard, D Fisher, S Fong, D Hsiao, C Koseoglu, S Moy, AM Shapiro, S Verdon, L Watson, T Frane, S Friedman, N Seino, J McDonnell, S Paez, E Perea, C Sloan, D Smith, E Soto, L Stelly-Leonard, A Moore, B Bolden, J Umana, S Smith, J Quinn, N Carlson, N Suckow, M Croteau, A Kran, B Ramsey, J Weissberg, E Kurtz, D Laby, D Lyons, S Chery, M Gonzalez, L Braverman, E Crowley, S Dennehy, P Jaramillo, B Schmidt, P Kulp, MT Biddle, M Haynes, J Hudson, J Edwards, K Gebhart, H Hickson, A Jenkins, L Anderson, S Evans, N Henry, J Hertle, R Hutchinson, J Toole, A Earley, M Crawford, S Reuter, K Johnson, K Haas, B James, T Martin, D Dorton, S Grace, Y Hisle, T Jones, C Smith, B Bower, R Ciner, E Duson, A Parke, L Boas, M Burgess, S Copenhaven, P Francis, E Gallaway, M Quinn, G Schwartz, J Scombordi-Raghu, B Swiatocha, J Zikoski, E Lin, J Menacker, S Little, R Moss, G Figueroa, J Hall, B Nesmith, E Gold, G Ciner, D Jordan, E Harvey, D Cyert, L Cheatham, L Chambless, A Beats, C Coy, D Long, J Carter, J Rice, S Dunn, J Harrington, E Trimble, L Dreadfulwater, S McCully, C Wyers, R Bingham, E Taylor, V Byfield, G Gower, P Roastingear, K Ross, E Schmidt, P Haas, B Maguire, M Peskin, E Brightwell-Arnold, M Holmes, C James, A Khvatov, A O'Brien, L Whearry, C Ying, GS Redford, M AF Schmidt, P Ciner, E Cyert, L Dobson, V Kulp, MT Maguire, M Moore, B Orel-Bixler, D Peskin, E Quinn, G Redford, M Schultz, J Beck, R Cotter, S Holmes, J Shipp, M West, S Schmidt, P Maguire, M Dobson, V Quinn, G Ciner, E Cyert, L Kulp, MT Moore, B Orel-Bixler, D Redford, M Ying, GS Orel-Bixler, D Qualley, P Howard, D Fisher, S Fong, D Hsiao, C Koseoglu, S Moy, AM Shapiro, S Verdon, L Watson, T Frane, S Friedman, N Seino, J McDonnell, S Paez, E Perea, C Sloan, D Smith, E Soto, L Stelly-Leonard, A Moore, B Bolden, J Umana, S Smith, J Quinn, N Carlson, N Suckow, M Croteau, A Kran, B Ramsey, J Weissberg, E Kurtz, D Laby, D Lyons, S Chery, M Gonzalez, L Braverman, E Crowley, S Dennehy, P Jaramillo, B Schmidt, P Kulp, MT Biddle, M Haynes, J Hudson, J Edwards, K Gebhart, H Hickson, A Jenkins, L Anderson, S Evans, N Henry, J Hertle, R Hutchinson, J Toole, A Earley, M Crawford, S Reuter, K Johnson, K Haas, B James, T Martin, D Dorton, S Grace, Y Hisle, T Jones, C Smith, B Bower, R Ciner, E Duson, A Parke, L Boas, M Burgess, S Copenhaven, P Francis, E Gallaway, M Quinn, G Schwartz, J Scombordi-Raghu, B Swiatocha, J Zikoski, E Lin, J Menacker, S Little, R Moss, G Figueroa, J Hall, B Nesmith, E Gold, G Ciner, D Jordan, E Harvey, D Cyert, L Cheatham, L Chambless, A Beats, C Coy, D Long, J Carter, J Rice, S Dunn, J Harrington, E Trimble, L Dreadfulwater, S McCully, C Wyers, R Bingham, E Taylor, V Byfield, G Gower, P Roastingear, K Ross, E Schmidt, P Haas, B Maguire, M Peskin, E Brightwell-Arnold, M Holmes, C James, A Khvatov, A O'Brien, L Whearry, C Ying, GS Redford, M CA Vision Preschoolers Study Grp TI Comparison of preschool vision screening tests as administered by licensed eye care professionals in the vision in preschoolers study SO OPHTHALMOLOGY LA English DT Article ID AMBLYOGENIC FACTORS; CLINICAL-EVALUATION; AMBLYOPIA TREATMENT; MTI PHOTOSCREENER; CHILDREN; PHOTOREFRACTOR; AUTOREFRACTOR; STRABISMUS; PROTOCOL/; PROGRAMS AB Purpose: To compare 11 preschool vision screening tests administered by licensed eye care professionals (LEPs; optometrists and pediatric ophthalmologists). Design: Multicenter, cross-sectional study. Participants: A sample (N = 2588) of 3- to 5-year-old children enrolled in Head Start was selected to over-represent children with vision problems. Methods: Certified LEPs administered 11 commonly used or commercially available screening tests. Results from a standardized comprehensive eye examination were used to classify children with respect to 4 targeted conditions: amblyopia, strabismus, significant refractive error, and unexplained reduced visual acuity (VA). Main Outcome Measures: Sensitivity for detecting children with greater than or equal to1 targeted conditions at selected levels of specificity was the primary outcome measure. Sensitivity also was calculated for detecting conditions grouped into 3 levels of importance. Results: At 90% specificity, sensitivities of noncycloplegic retinoscopy (NCR) (64%), the Retinomax Autorefractor (63%), SureSight Vision Screener (63%), and Lea Symbols test (61%) were similar. Sensitivities of the Power Refractor II(54%) and HOTV VA test (54%) were similar to each other. Sensitivities of the Random Dot E stereoacuity (42%) and Stereo Smile II (44%) tests were similar to each other and lower (P<0.0001) than the sensitivities of NCR, the 2 autorefractors, and the Lea Symbols test. The cover-uncover test had very low sensitivity (16%) but very high specificity (98%). Sensitivity for conditions considered the most important to detect was 80% to 90% for the 2 autorefractors and NCR. Central interpretations for the MTI and iScreen photoscreeners each yielded 94% specificity and 37% sensitivity, At 94% specificity, the sensitivities were significantly better for NCR, the 2 autorefractors, and the Lea Symbols VA test than for the 2 photoscreeners for detecting = greater than or equal to1 targeted conditions and for detecting the most important conditions. Conclusions: Screening tests administered by LEPs vary widely in performance. With 90% specificity, the best tests detected only two thirds of children having !l targeted conditions, but nearly 90% of children with the most important conditions. The 2 tests that use static photorefractive technology were less accurate than 3 tests that assess refractive error in other ways. These results have important implications for screening preschoolaged children. (C) 2004 by the American Academy of Ophthalmology. C1 Ohio State Univ, Coll Optometry, Vis Preschoolers Study Ctr, Columbus, OH 43218 USA. NEI, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Schmidt, P (reprint author), Ohio State Univ, Coll Optometry, Vis Preschoolers Study Ctr, 320 W 10th Ave,POB 182342, Columbus, OH 43218 USA. RI Toole, Andrew/I-2450-2016 FU NEI NIH HHS [U10 EY 12547, U10 EY 12534, U10 EY 12545, U10 EY 12550, U10 EY 12644, U10 EY 12647, U10 EY 12648] NR 36 TC 141 Z9 144 U1 1 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0161-6420 J9 OPHTHALMOLOGY JI Ophthalmology PD APR PY 2004 VL 111 IS 4 BP 637 EP 650 DI 10.1016/j.ophtha.2004.01.022 PG 14 WC Ophthalmology SC Ophthalmology GA 809BX UT WOS:000220613300005 PM 15051194 ER PT J AU Gannot, I Garashi, A Chernomordik, V Gandjbachkhe, A AF Gannot, I Garashi, A Chernomordik, V Gandjbachkhe, A TI Quantitative optical imaging of the pharmacokinetics of fluorescent-specific antibodies to tumor markers through tissuelike turbid media SO OPTICS LETTERS LA English DT Article ID IN-VIVO; CONTRAST AGENTS AB Fluorescent optical imaging of tumors deep within tissue depends on specific binding of antibodies to the tumors' surface markers. These fluorescent antibodies propagating in the vicinity of the tumor can be attached to and (or) diffused away from it. We illustrate application of a new tool, based on the random-walk theory in turbid media, for extracting the pharmacokinetics of these fluorescent antibodies by data deconvolution, excluding the effect of upper turbid tissue layers. (C) 2004 Optical Society of America. C1 Tel Aviv Univ, Fac Engn, Dept Biomed Engn, IL-69978 Tel Aviv, Israel. NICHHD, NIH, Bethesda, MD 20892 USA. RP Gannot, I (reprint author), Tel Aviv Univ, Fac Engn, Dept Biomed Engn, IL-69978 Tel Aviv, Israel. NR 12 TC 14 Z9 14 U1 0 U2 0 PU OPTICAL SOC AMER PI WASHINGTON PA 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA SN 0146-9592 J9 OPT LETT JI Opt. Lett. PD APR 1 PY 2004 VL 29 IS 7 BP 742 EP 744 DI 10.1364/OL.29.000742 PG 3 WC Optics SC Optics GA 805JN UT WOS:000220362700028 PM 15072377 ER PT J AU Luethje, LGC Boennemann, C Goldfarb, L Goebel, HH Halle, M AF Luethje, LGC Boennemann, C Goldfarb, L Goebel, HH Halle, M TI Prophylactic implantable cardioverter defibrillator placement in a sporadic desmin related myopathy and cardiomyopathy SO PACE-PACING AND CLINICAL ELECTROPHYSIOLOGY LA English DT Article DE desmin related myopathy; cardiomyopathy; ICD ID GENE AB LUETHJE, L.G.C., ET AL.: Prophylactic Implantable Cardioverter Defibrillator Placement in a Sporadic Desmin Related Myopathy and Cardiomyopathy. Desminopathy is a neuromuscular disorder associated with the accumulation of the protein desmin. This article reports a case of a man with a mutation in the desmin gene suffering from cardiomyopathy and skeletal myopathy. This patient underwent implantable cardioverter defibrillator (ICD) implantation for prognostic considerations and subsequently developed a sustained ventricular tachycardia (SVT). While nonsustained VTs (NSVT) have previously been reported, this is the first time that a SVT could be seen in a patient with this disease. C1 Univ Gottingen, Dept Cardiol & Pneumol, D-3400 Gottingen, Germany. Tech Univ Munich, Dept Prevent Rehabil & Sports Med, D-8000 Munich, Germany. NINDS, Clin Neurogenet Unit, Bethesda, MD 20892 USA. Univ Mainz, Dept Neuropathol, D-6500 Mainz, Germany. Childrens Hosp, Div Neurol, Philadelphia, PA 19104 USA. RP Luethje, LGC (reprint author), Herzzentrum, Abt Kardiol & Pneumol, Robert Koch Str 40, D-37075 Gottingen, Germany. EM LarsLuethje@t-online.de NR 8 TC 12 Z9 13 U1 0 U2 0 PU BLACKWELL FUTURA PUBLISHING, INC PI MALDEN PA 350 MAIN STREET, MALDEN, MA 01248-5018 USA SN 0147-8389 J9 PACE JI PACE-Pacing Clin. Electrophysiol. PD APR PY 2004 VL 27 IS 4 BP 559 EP 560 DI 10.1111/j.1540-8159.2004.00484.x PG 2 WC Cardiac & Cardiovascular Systems; Engineering, Biomedical SC Cardiovascular System & Cardiology; Engineering GA 814DM UT WOS:000220955400026 PM 15078418 ER PT J AU Ahmed, A Gilbert-Barness, E Lacson, A AF Ahmed, A Gilbert-Barness, E Lacson, A TI Expression of c-kit in Ewing family of tumors: A comparison of different immunohistochemical protocols SO PEDIATRIC AND DEVELOPMENTAL PATHOLOGY LA English DT Article DE antigen retrieval; c-kit; Ewing sarcoma; Ki-67 ID STEM-CELL FACTOR; ANTIGEN RETRIEVAL; SOLID TUMORS; SARCOMA; MUTATIONS; IMATINIB; PROTEINS; TISSUES; CD117; BONE AB Ewing sarcoma is a small round blue cell tumor with a high incidence of metastasis and poor survival. The tyrosine kinase receptor, c-kit, is a growth factor receptor that is expressed in a variety of tumors including Ewing sarcoma. Blockade of c-kit by imatinib mesylate (Gleevec; Novartis Pharmaceuticals Corp, East Hanover, NJ) has been successfully used in the treatment of chronic myelogenous leukemia and gastrointestinal tumors. Detection of c-kit expression in Ewing sarcoma indicates a possible role of c-kit in tumor progression and a potential use of anti-c-kit therapy in Ewing sarcoma. Ki-67 is a proliferation marker found at all stages of the cell cycle. Expression of c-kit and Ki-67 was studied in 17 patients with Ewing sarcoma. Sections from paraffin-embedded tumor samples were immunostained, using standard immunohistochemical protocols, with c-kit and Ki-67 monoclonal antibodies, polyclonal c-kit antibody without antigen retrieval, and c-kit polyclonal antibody with antigen retrieval. Eleven out of 17 cases (65%) stained with c-kit monoclonal antibody; the staining was diffuse in 6/17 (35%) cases. C-kit expression did not correlate with Ki-67 proliferation rates. Using the polyclonal c-kit-antibody without antigen retrieval methods, c-kit expression was demonstrated in 1/11 (9%) cases. Incorporating antigen retrieval methods, c-kit expression increased to 53%. Concordance between monoclonal antibodies in detecting c-kit expression was observed in 12/17 cases (71%). We conclude that c-kit is variably expressed in Ewing sarcoma, using either monoclonal or polyclonal antibodies. Detection of c-kit expression in Ewing sarcoma improves with the use of antigen retrieval methods. C1 NCI, Sect Pediat Tumor Biol & Ultrastruct Pathol, Bethesda, MD 20892 USA. Univ S Florida, Dept Pathol, Tampa, FL USA. RP Ahmed, A (reprint author), NCI, Sect Pediat Tumor Biol & Ultrastruct Pathol, NIH Bldg 10,Rm 2A10,10 Ctr Dr, Bethesda, MD 20892 USA. EM ahmeda@mail.nih.gov OI Ahmed, Atif/0000-0002-8791-5785 NR 23 TC 18 Z9 18 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING STREET, NEW YORK, NY 10013 USA SN 1093-5266 J9 PEDIATR DEVEL PATHOL JI Pediatr. Dev. Pathol. PD APR PY 2004 VL 7 IS 4 BP 342 EP 347 DI 10.1007/s10024-002-0077-y PG 6 WC Pathology; Pediatrics SC Pathology; Pediatrics GA 849IQ UT WOS:000223532300005 PM 15383930 ER PT J AU Byrne, J Fears, TR Mills, JL Zeltzer, LK Sklar, C Meadows, AT Reaman, GH Robison, LL AF Byrne, J Fears, TR Mills, JL Zeltzer, LK Sklar, C Meadows, AT Reaman, GH Robison, LL TI Fertility of long-term male survivors of acute lymphoblastic leukemia diagnosed during childhood SO PEDIATRIC BLOOD & CANCER LA English DT Article DE cohort study; fertility; male childhood leukemia survivors; radiotherapy ID CHILDRENS CANCER GROUP; CRANIAL IRRADIATION; GONADAL DAMAGE; CHEMOTHERAPY; MALIGNANCIES; ANOMALIES; BOYS AB Fertility impairments among men treated during childhood for cancer are known to occur after some, but not all, types of anti-cancer therapy. This is the first study to evaluate proven fertility among adult male survivors of childhood acute lymphoblastic leukemia (ALL). In a retrospective cohort study, proven fertility (ever fathered a pregnancy) was evaluated by self-report among 213 men treated for ALL before age 18 on protocols of the Children's Cancer Group (CCG). Controls (N = 145) were drawn from among male siblings. Overall, with a proportional hazards analysis, proven fertility of male survivors was not different from that of controls (relative fertility (RF) = 0.95, 95% CI 0.63-1.43). However, married men treated before age 10 with high dose (24 cGy) cranial radiotherapy (RT), without spinal RT, had only 9% of the fertility of controls (Relative risk, RR = 0.09, 95% CI 0.01-0.82). High dose cranial RT at older ages was not associated with a statistically significant fertility deficit (RR = 0.56, 95% CI 0.25-1.28). In this first study of proven fertility among men treated for childhood leukemia, the majority of survivors showed no evidence of fertility impairment compared to controls. However, men treated at a young age with high dose cranial RT may have impaired fertility. These results suggest that further investigation of men with these treatments is needed to confirm and extend these findings. (C) 2003 Wiley-Liss, Inc. C1 Childrens Natl Med Ctr, Dept Hematol Oncol, Washington, DC 20010 USA. Univ Minnesota, Ctr Canc, Minneapolis, MN USA. Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA. Univ Calif Los Angeles, Los Angeles, CA USA. Childrens Canc Grp, Arcadia, CA USA. Dept Hlth & Human Serv, Washington, DC USA. NIH, Bethesda, MD 20892 USA. RP Byrne, J (reprint author), Childrens Natl Med Ctr, Dept Hematol Oncol, 111 Michigan Ave NW, Washington, DC 20010 USA. EM jbyrne@cnmc.org OI Zeltzer, Lonnie/0000-0001-9306-9450 NR 24 TC 30 Z9 32 U1 1 U2 4 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 1545-5009 J9 PEDIATR BLOOD CANCER JI Pediatr. Blood Cancer PD APR PY 2004 VL 42 IS 4 BP 364 EP 372 DI 10.1002/pbc.10449 PG 9 WC Oncology; Hematology; Pediatrics SC Oncology; Hematology; Pediatrics GA 812WF UT WOS:000220868900011 PM 14966835 ER PT J AU Mohan, AK Braun, MM Ellenberg, S Hedje, J Cote, TR AF Mohan, AK Braun, MM Ellenberg, S Hedje, J Cote, TR TI Deaths among children less than two years of age receiving palivizumab: an analysis of comorbidities SO PEDIATRIC INFECTIOUS DISEASE JOURNAL LA English DT Article DE synagis; palivizumab; respiratory syncytial virus ID RESPIRATORY SYNCYTIAL VIRUS; REDUCES HOSPITALIZATION; MONOCLONAL-ANTIBODY; INFANT-MORTALITY; PROPHYLAXIS; MEDI-493 AB Background. Palivizumab (Synagis) is used for prophylaxis against respiratory syncytial virus infection among children at high risk for respiratory syncytial virus disease. A number of deaths after palivizumab use among children <2 years have been reported to the Food and Drug Administration. We assessed available information, including the extent to which preexisting medical conditions may have put these children at higher than normal risk of death. Methods. We reviewed reports of deaths to the Food and Drug Administration (June 1998 to December 2001) among children <2 years of age who received palivizumab. Results. There were 133 deaths reported after palivizumab use. Median age at death was 5 months, and 54% of the children were male. At least one congenital anomaly was reported in 85 cases (64%), and 44% of cases had multiple anomalies. Of the 100 cases with reported gestational age at birth, 36% were severely premature (<28 weeks), 48% were moderately premature (28 to 36 weeks) and 16% had normal gestational age. Only 2% of all cases were full term and were born without congenital anomalies; 50% had both conditions, 34% had prematurity alone and 14% had congenital anomalies alone. A cause of death was reported for 88 (66%) cases; most (38%) died from their congenital anomalies or from respiratory infections (23%). Conclusions. Most children dying after palivizumab treatment were at increased risk of death; many had multiple congenital anomalies and/or premature birth. Patterns of outcomes and the reported medical course did not suggest that palivizumab further elevated the risk of death. Current data do not alter the safety and efficacy assessment that led to the licensure of palivizumab. C1 US FDA, Ctr Biol Evaluat & Res, Off Biostat & Epidemiol, Rockville, MD 20852 USA. NIAID, Off Global Affairs, NIH, Bethesda, MD 20892 USA. RP Mohan, AK (reprint author), US FDA, Ctr Biol Evaluat & Res, Off Biostat & Epidemiol, 1404 Rockville Pike,Suite 200S, Rockville, MD 20852 USA. EM mohan@cber.fda.gov NR 24 TC 7 Z9 7 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0891-3668 J9 PEDIATR INFECT DIS J JI Pediatr. Infect. Dis. J. PD APR PY 2004 VL 23 IS 4 BP 342 EP 345 DI 10.1097/00006454-200404000-00013 PG 4 WC Immunology; Infectious Diseases; Pediatrics SC Immunology; Infectious Diseases; Pediatrics GA 812YD UT WOS:000220873900012 PM 15071290 ER PT J AU Norman, AC Drinkard, B McDuffie, JR Fredricks, A Sebring, N Mercado, AB Yanovski, JA AF Norman, AC Drinkard, B McDuffie, JR Fredricks, A Sebring, N Mercado, AB Yanovski, JA TI The influence of body composition on exercise performance in overweight children and adolescents SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIH, Unit Growth & Obes, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 14 BP 3A EP 3A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100031 ER PT J AU Saluja, G Iachan, R Scheidt, PC Overpeck, MD Sun, WY Giedd, JN AF Saluja, G Iachan, R Scheidt, PC Overpeck, MD Sun, WY Giedd, JN TI Prevalence and risk factors for depression among young adolescents SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHHD, Div Epidemiol Stat & Prevent Res, Bethesda, MD 20892 USA. Macro Int, Calverton, MD USA. US Hlth Resources & Serv Adm, US Maternal & Child Hlth Bur, Rockville, MD 20857 USA. NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. RI Giedd, Jay/A-3080-2008; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015 OI Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978 NR 0 TC 0 Z9 0 U1 1 U2 5 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 40 BP 7A EP 7A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100057 ER PT J AU Anderson, KM Jenkins, RR El-Khorazaty, N Schwartz, DA Yao, Q Walker, LR Davis, AM AF Anderson, KM Jenkins, RR El-Khorazaty, N Schwartz, DA Yao, Q Walker, LR Davis, AM TI Attitudes toward and perceptions of abstinence and sexual behavior among African American fifth graders SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Howard Univ, NIH, DC Initiat, Washington, DC 20059 USA. Res Triangle Inst Int, Rockville, MD USA. Georgetown Univ, Med Ctr, Washington, DC 20057 USA. Res Triangle Inst Int, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 57 BP 10A EP 10A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100074 ER PT J AU Chen, AM Rogan, WJ AF Chen, AM Rogan, WJ TI Breastfeeding and post-neonatal mortality in the US SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIEHS, Epidemiol Branch, Res Triangle Pk, NC USA. RI Rogan, Walter/I-6034-2012 OI Rogan, Walter/0000-0002-9302-0160 NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 198 BP 35A EP 35A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100215 ER PT J AU Finer, N Carlo, W Duara, S Fanaroff, A Donovan, E AF Finer, N Carlo, W Duara, S Fanaroff, A Donovan, E CA NICHD TI Randomized pilot trial of delivery room CPAP in the ELBW infant SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Network, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 11 BP XLII EP XLIII PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100012 ER PT J AU McKinney, RE Cruz, MLS Powell, C Hughes, M Oleske, JM Winter, H Elgie, C Pardue, L Wolf, D Ortiz-Pujols, S Calles, NR McNamara, J Moye, J AF McKinney, RE Cruz, MLS Powell, C Hughes, M Oleske, JM Winter, H Elgie, C Pardue, L Wolf, D Ortiz-Pujols, S Calles, NR McNamara, J Moye, J TI International formula-based nutritional intervention for infants born to HIV-infected women SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Duke Univ, Med Ctr, Durham, NC 27706 USA. Hosp Servidores Estado, Rio De Janeiro, Brazil. Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. Univ Med & Dent New Jersey, Newark, NJ 07103 USA. Massachusetts Gen Hosp, Boston, MA 02114 USA. Frontier Sci Tech Res Fdn, Amherst, NY USA. NIAID, NIH, DHHS, Bethesda, MD 20892 USA. Ross Prod Div, Columbus, OH USA. Soc Sci Syst, Silver Spring, MD USA. Texas Childrens Hosp, Houston, TX 77030 USA. RI Oleske, James/C-1951-2016 OI Oleske, James/0000-0003-2305-5605 NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 16 BP XLIII EP XLIII PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100017 ER PT J AU Panigrahi, P Satpathy, R Nanda, N Pradhan, L Mohapatra, S Morris, JG Johnson, J Wright, L Poole, K Parida, S Gewolb, I AF Panigrahi, P Satpathy, R Nanda, N Pradhan, L Mohapatra, S Morris, JG Johnson, J Wright, L Poole, K Parida, S Gewolb, I TI Reduction of infant and neonatal deaths by village level education and training and early referral to the hospital in an Indian community setting SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Bethesda, MD USA. Capital Hosp, Bhubaneswar, Orissa, India. Univ Maryland, Baltimore, MD 21201 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 321 BP 57A EP 57A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100338 ER PT J AU LaGasse, LL Lester, BM Seifer, R Bauer, CR Shankaran, S Bada, HS Poole, K Wright, L Smeriglio, V Liu, J AF LaGasse, LL Lester, BM Seifer, R Bauer, CR Shankaran, S Bada, HS Poole, K Wright, L Smeriglio, V Liu, J TI Prenatal cocaine exposure and cognitive development at school age SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, NIH, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 390 BP 69A EP 69A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100407 ER PT J AU Snider, LA Swedo, SE AF Snider, LA Swedo, SE TI Antibiotic prophylaxis against group A beta-hemolytic streptococci with azithromycin or penicillin for childhood-onset neuropsychiatric disorders SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIMH, Pediat & Dev Neuropsychiat Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 392 BP 69A EP 69A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100409 ER PT J AU Bada, HS Twomey, J Bursi, C Langer, J Lester, B Bauer, C Shankaran, S LaGasse, L Wright, L Smeriglio, V AF Bada, HS Twomey, J Bursi, C Langer, J Lester, B Bauer, C Shankaran, S LaGasse, L Wright, L Smeriglio, V TI Behavior problems and adaptive skills of children in out-of-home placement because of prenatal drug exposure SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 410 BP 72A EP 73A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100427 ER PT J AU Arnold, LE Lindsay, RL Elliott, M Vitiello, B Hechtman, L Elliott, GR Molina, B Newcorn, J Epstein, JN Wigal, T AF Arnold, LE Lindsay, RL Elliott, M Vitiello, B Hechtman, L Elliott, GR Molina, B Newcorn, J Epstein, JN Wigal, T TI Gestational and amblent tobacco smoke as predictor of ADHD severity, comorbid ODD/CD, and treatment response in the MTA: Relation to sociodemographics SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Ohio State Univ, Columbus, OH 43210 USA. St Josephs Hosp, Childrens Hlth Ctr, Phoenix, AZ USA. St Louis Univ, St Louis, MO 63103 USA. NIMH, Rockville, MD 20857 USA. Montreal Childrens Hosp, Montreal, PQ H3H 1P3, Canada. Univ Calif San Francisco, San Francisco, CA 94143 USA. Univ Pittsburgh, Pittsburgh, PA USA. Mt Sinai Med Ctr, New York, NY 10029 USA. Duke Univ, Med Ctr, Durham, NC USA. Univ Calif Irvine, Irvine, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 460 BP 81A EP 81A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100477 ER PT J AU Horn, IB Brenner, R Cheng, TL AF Horn, IB Brenner, R Cheng, TL TI Beliefs about initiation of toilet training - Are there SES or racial differences? SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Childrens Natl Med Ctr, Washington, DC USA. NICHHD, Div Epidemiol Stat & Prevent Res, Bethesda, MD USA. Johns Hopkins Sch Med, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 481 BP 85A EP 85A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100498 ER PT J AU Doe, EC Wu, YJ Liu, CY Lo, C AF Doe, EC Wu, YJ Liu, CY Lo, C TI High throughput genotype based screen of EMS-induced connexin mutations in mouse embryonic stem cells using denaturing HPLC analysis SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Natl Heart Lung & Blood Inst, Lab Dev Biol, NIH, Bethesda, MD 20814 USA. Natl Naval Med Res Inst, Bethesda, MD USA. Uniformed Serv Univ Hlth Sci, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 536 BP 94A EP 94A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100553 ER PT J AU Shah, PK Miller, NH Marosy, BA Justice, CM Wilson, AF AF Shah, PK Miller, NH Marosy, BA Justice, CM Wilson, AF TI Identification of single nucleotide polymorphims potentially linked to familial idiopathic scoliosis SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Johns Hopkins Univ, Baltimore, MD USA. NHGRI, Genometr Sect, NIH, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 541 BP 95A EP 95A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100558 ER PT J AU Siegfried, BH Chatterjee, B Yu, C Leatherbury, L Lo, CW AF Siegfried, BH Chatterjee, B Yu, C Leatherbury, L Lo, CW TI ENU mutagenized mice with persistent truncus arteriosus and interrupted aortic arch caused by a mutation on proximal chromosome 5 SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Natl Naval Med Res Inst, NIH, Bethesda, MD 20010 USA. Natl Heart Lung & Blood Inst, Bethesda, MD USA. Uniformed Serv Univ Hlth Sci, Bethesda, MD USA. Childrens Natl Med Ctr, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 543 BP 96A EP 96A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100560 ER PT J AU Lang, DM Holland, SM Marciano, BE Khuns, DB Merke, DP AF Lang, DM Holland, SM Marciano, BE Khuns, DB Merke, DP TI Short stature in patients with chronic granulomatous disease is associated with X-linked genotype, granulomatous gastrointestinal disease and phagocyte metabolic activity SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIH, Warren Grant Magnuson Clin Ctr, Bethesda, MD 20892 USA. NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 583 BP 102A EP 102A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100616 ER PT J AU Robatham, D Schoeller, DA Mercado, AB Yanovski, JA AF Robatham, D Schoeller, DA Mercado, AB Yanovski, JA TI Body fat in children is underestimated by the QDR-4500 DXA relative to deuterium dilution SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIH, Unit Growth & Obesity, Bethesda, MD USA. Univ Madison, Dept Nutr Sci, Madison, WI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 833 BP 148A EP 148A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100866 ER PT J AU Stergiopoulos, SG Batista, D Bauer, A Meoli, E Weinberg, F Smith, A Stratakis, CA AF Stergiopoulos, SG Batista, D Bauer, A Meoli, E Weinberg, F Smith, A Stratakis, CA TI Protein kinase A (PIKA) function in visceral fat from Cushing syndrome patients with and without germline PKA regulatory subunit type 1A (PRK4R1A) inactivating mutations SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, SEGEN DEB, NIH, Bethesda, MD USA. NICHD, UGM DEB, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 838 BP 149A EP 149A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100871 ER PT J AU Griffin, KJ Kirschner, LS Weinberg, FD Claflin, ES Stergiopoulos, SG Carney, JA Stratakis, CA AF Griffin, KJ Kirschner, LS Weinberg, FD Claflin, ES Stergiopoulos, SG Carney, JA Stratakis, CA TI A mouse model of inherited Cushing syndrome SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, DEB, Sect Genet & Endocrinol, Bethesda, MD USA. Ohio State Univ, Columbus, OH 43210 USA. Mayo Clin, Rochester, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 849 BP 151A EP 151A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100882 ER PT J AU Nelson, CA Batista, DL Stratakis, CA AF Nelson, CA Batista, DL Stratakis, CA TI LDH in children with Cushing's disease SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, NIH, Bethesda, MD USA. Case Western Reserve Univ, Sch Med, Cleveland, OH 44106 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 867 BP 154A EP 154A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100900 ER PT J AU Watterberg, KL AF Watterberg, KL TI A cosyntropin (ACTH) dose of 0.1mcg/kg is inadequate to test adrenal function in extremely birth weight (ELBW) infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ New Mexico, PROPHET Study Grp, NICHD ROI 38540, Albuquerque, NM 87131 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 866 BP 154A EP 154A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100899 ER PT J AU Ross, JL Quigley, CA Rose, SR Sandberg, DE Leschek, EW Baron, J Chipman, JJ Crowe, B Cutler, GB AF Ross, JL Quigley, CA Rose, SR Sandberg, DE Leschek, EW Baron, J Chipman, JJ Crowe, B Cutler, GB TI Psychological adaptation in children with idiopathic short stature treated with growth hormone or placebo SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Thomas Jefferson Univ, Philadelphia, PA 19107 USA. DuPont Hosp Children, Philadelphia, PA USA. Eli Lilly & Co, Indianapolis, IN 46285 USA. NICHD, DEB, NIH, Bethesda, MD USA. SUNY Buffalo, Buffalo, NY 14260 USA. Cincinnati Childrens Hosp, Med Ctr, Cincinnati, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 893 BP 158A EP 159A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100926 ER PT J AU Touger, L Looker, HC Krakoff, J Cook, V Knowler, WC AF Touger, L Looker, HC Krakoff, J Cook, V Knowler, WC TI Early growth pattern of offspring of diabetic mothers SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIDDKD, Diabet Arthrit Epidemiol Sect, Phoenix, AZ USA. Phoenix Childrens Hosp, Phoenix, AZ USA. Dept Publ Hlth Nursing, Sacaton, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 890 BP 158A EP 158A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100923 ER PT J AU Subramanian, S Katz, K Rodan, M Milligan, R Blake, S El-Mohandes, A White, D Schwartz, D El-Khorazaty, N Kiely, M AF Subramanian, S Katz, K Rodan, M Milligan, R Blake, S El-Mohandes, A White, D Schwartz, D El-Khorazaty, N Kiely, M TI Risks associated with short inter-pregnancy interval (IPI)among urban, low-income, African-American (AA) women SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Georgetown Univ, Med Ctr, Washington, DC 20007 USA. George Washington Univ, Washington, DC 20052 USA. RTI Int, Rockville, MD USA. Howard Univ, Washington, DC 20059 USA. NICHD, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 933 BP 165A EP 165A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100965 ER PT J AU Subramanian, S Katz, KS Rodan, M Schwartz, DA El-Khorazaty, N Kiely, M AF Subramanian, S Katz, KS Rodan, M Schwartz, DA El-Khorazaty, N Kiely, M TI Addressing maternal depression to improve infant outcomes for low income African Americans SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Georgetown Univ, Med Ctr, Washington, DC 20007 USA. RTI Int, Rockville, MD USA. NICHD, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 941 BP 167A EP 167A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100973 ER PT J AU El-Mohandes, AAE Blake, SM Kiely, M El-Khorazaty, N Murray, K Joseph, JG AF El-Mohandes, AAE Blake, SM Kiely, M El-Khorazaty, N Murray, K Joseph, JG TI Smoking during pregnancy, the tip of an iceberg: Associated behavioral risks and reproductive morbidities SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 George Washington Univ, Sch Publ Hlth & Hlth Serv, Washington, DC USA. NICHHD, Rockville, MD USA. Res Triangle Inst, Rockville, MD USA. Childrens Natl Med Ctr, Washington, DC 20010 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 950 BP 168A EP 168A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591100982 ER PT J AU Satpathy, R Panda, P Misra, P Shinkre, N Chandel, D Sharma, N Pote, M Chaudhry, R Mohapatra, S Pradhan, L Jayakar, A Johnson, J Gewolb, I Wright, L Poole, K Kandefer, S Parida, S Panigrahi, P AF Satpathy, R Panda, P Misra, P Shinkre, N Chandel, D Sharma, N Pote, M Chaudhry, R Mohapatra, S Pradhan, L Jayakar, A Johnson, J Gewolb, I Wright, L Poole, K Kandefer, S Parida, S Panigrahi, P TI Role of external and internal quality control measures on data quality in a multicenter study of neonatal sepsis in India SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ Maryland, Baltimore, MD 21201 USA. Nair Hosp, Bombay, Maharashtra, India. Capital Hosp, Bhubaneswar, Orissa, India. AIIMS, New Delhi, India. NICHD, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 989 BP 175A EP 175A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101021 ER PT J AU Feng, NP Young, S Aguilera, G Adler-Wailes, D Yanovski, JA AF Feng, NP Young, S Aguilera, G Adler-Wailes, D Yanovski, JA TI Co-occurrence of two partially inactivating polymorphisms of the melanocortin receptor (MC3R) is associated with pediatric-onset SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIH, Unit Growth & Obes, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1024 BP 181A EP 182A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101056 ER PT J AU Fredricks, A Yanoff, L Norman, AC Semega-Janneh, M McDuffie, JR Yanovski, JA AF Fredricks, A Yanoff, L Norman, AC Semega-Janneh, M McDuffie, JR Yanovski, JA TI The high prevalence of iron deficiency among overweight adolescents explained by differences in dietary iron intake, or by socio-demographic factors? SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIH, Unit Growth & Obes, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1028 BP 182A EP 182A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101060 ER PT J AU Nader, PR O'Brien, M Houts, R AF Nader, PR O'Brien, M Houts, R CA NICHD Early Child Care TI Correlates of physical activity and body mass index in third grade children: NICHD early child care research network SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 Univ Calif San Diego, NICHD, Early Child Care Res Network, La Jolla, CA 92093 USA. Univ N Carolina, Greensboro, NC 27412 USA. RTI Int, Res Triangle Pk, NC USA. NICHD, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1216 BP 215A EP 215A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101265 ER PT J AU Mirza, N Kadow, K Yanovski, J AF Mirza, N Kadow, K Yanovski, J TI Latino parents' perceptions of childhood obesity: A qualitative study SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 Childrens Natl Med Ctr, Washington, DC 20010 USA. Natl Inst Hlth, DEB, Unit Growth & Obes, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1246 BP 220A EP 220A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101295 ER PT J AU Blake, SM Kiely, M Gard, C El-Mohandes, AE El-Khorazaty, MN Walker, L Milligan, R White, D AF Blake, SM Kiely, M Gard, C El-Mohandes, AE El-Khorazaty, MN Walker, L Milligan, R White, D TI Pregnancy intendedness, happiness, maternal risk and their correlates during the first and second trimester of pregnancy SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 George Washington Univ, Med Ctr, Washington, DC 20037 USA. Natl Inst Child Hlth & Human Dev, Rockville, MD USA. Res Triangle Inst, Rockville, MD USA. Georgetown Univ, Med Ctr, Washington, DC 20007 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1402 BP 247A EP 247A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101451 ER PT J AU Cabral, WA Makareeva, E Leikin, S Colige, A Letocha, AD Ty, J Yeowell, HH Pals, G Marini, JC AF Cabral, WA Makareeva, E Leikin, S Colige, A Letocha, AD Ty, J Yeowell, HH Pals, G Marini, JC TI Mutations in the first 90 residues of the alpha1(l) collagen chain cause combined Ehlers-Danlos and OI by interference with N-propeptide processing SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 NICHD, Bone & Extracellular Matrix Branch, NIH, Bethesda, MD USA. Univ Liege, Liege, Belgium. Duke Univ, Med Ctr, Div Dermatol, Durham, NC USA. VU Med Ctr, Amsterdam, Netherlands. RI Pals, Gerard/A-5198-2011 NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1542 BP 271A EP 271A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101591 ER PT J AU Tang, J Liu, PC Steinbach, PJ Luban, NL Kaler, SG AF Tang, J Liu, PC Steinbach, PJ Luban, NL Kaler, SG TI Expression studies and homology modeling of GPlb beta SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 NICHD, Unit Pediat Genet, NIH, Bethesda, MD USA. NIH, Ctr Informat Technol, Ctr Mol Modeling, Bethesda, MD USA. Childrens Natl Med Ctr, Dept Lab Med, Washington, DC 20010 USA. Childrens Natl Med Ctr, Dept Hematol Oncol, Washington, DC 20010 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1543 BP 271A EP 271A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101592 ER PT J AU Letocha, AD Cintas, HL Paul, SM Gerber, NL Marini, JC AF Letocha, AD Cintas, HL Paul, SM Gerber, NL Marini, JC TI Pamidronate in children with types III and IVOI: Lack of effect on function SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 NICHD, Bone & Extracellular Matrix Branch, NIH, Bethesda, MD USA. NIH, Rehabil Dept, Ctr Clin, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1548 BP 272A EP 272A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101597 ER PT J AU Uveges, TE Kozloff, KM Ty, JM Gronowicz, G Ledgard, F Goldstein, SA Marini, JC AF Uveges, TE Kozloff, KM Ty, JM Gronowicz, G Ledgard, F Goldstein, SA Marini, JC TI Alendronate treatment of Brtl mouse model for osteogenesis imperfecta increases bone strength by increasing bone volume but fails to improve femoral brittleness or mineralization SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 NICHD, BEMB, NIH, Bethesda, MD USA. Univ Michigan, Ortho Res Labs, Ann Arbor, MI 48109 USA. Univ Connecticut, Ctr Hlth, Farmington, CT USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S BP 272A EP 272A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101598 ER PT J AU Shanske, AL Bendavid, C Haddad, B Muencke, M AF Shanske, AL Bendavid, C Haddad, B Muencke, M TI Deletion of TGIF in child with Holoprosencephaly and dup18p SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 Albert Einstein Coll Med, Childrens Hosp Montefiore, Ctr Craniofacial Disorders, Bronx, NY USA. NHGRI, Med Genet Branch, NIH, Bethesda, MD USA. Georgetown Univ, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S BP 273A EP 273A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101604 ER PT J AU Howell, RR van Dyke, P Alexander, D AF Howell, RR van Dyke, P Alexander, D TI Newborn screening - Future promise SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 Univ Miami, Sch Med, Miami, FL 33152 USA. NRSA HHS, Maternal & Child Hlth Bur, Rockville, MD USA. NICHD, NIH, HHS, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1570 BP 276A EP 276A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101619 ER PT J AU Rimoin, AW Steinhoff, MC Oazi, S daCunha, ALA Hamza, HS Vince, A AF Rimoin, AW Steinhoff, MC Oazi, S daCunha, ALA Hamza, HS Vince, A TI Development of a clinical prediction rule for streptococcal pharyngitis in children on 3 continents SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 Johns Hopkins Sch Publ Hlth, Baltimore, MD USA. NICHD, NIH, Rockville, MD USA. Johns Hopkins Med Inst, Baltimore, MD 21205 USA. WHO, CH-1211 Geneva, Switzerland. Cairo Univ, Cairo, Egypt. Univ Infect Dis Hosp, Zagreb, Croatia. Fed Univ Rio De Janeiro, Rio De Janeiro, Brazil. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1585 BP 278A EP 279A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101634 ER PT J AU Rimoin, AW Vince, A Hamza, H da Cunha, ALA Chitale, R Oazi, S Steinhoff, MC AF Rimoin, AW Vince, A Hamza, H da Cunha, ALA Chitale, R Oazi, S Steinhoff, MC TI Evaluation of a rapid test for streptococcal pharyngitis in children in 3 countries SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies/Society-for-Pediatric-Research CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Cont Educ C1 Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA. NICHD, NIH, Rockville, MD USA. WHO, Child & Adolescent Hlth Dept, CH-1211 Geneva, Switzerland. Cairo Univ, Cairo, Egypt. Univ Infect Dis Hosp, Zagreb, Croatia. Univ Fed Rio de Janeiro, Rio De Janeiro, Brazil. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 W CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1586 BP 279A EP 279A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101635 ER PT J AU Rimoin, AW Hamza, HS Vince, A Qazi, S Steinhoff, MC AF Rimoin, AW Hamza, HS Vince, A Qazi, S Steinhoff, MC TI Variation in compliance with an oral treatment regimen for streptococcal pharyngitis in children in 2 countries SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies/Society-for-Pediatric-Research CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Cont Educ C1 Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA. NICHD, NIH, Rockville, MD USA. Cairo Univ, Cairo, Egypt. Univ Infect Dis Hosp, Zagreb, Croatia. WHO, Geneva, Switzerland. Johns Hopkins Med Inst, Baltimore, MD 21205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 W CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1587 BP 279A EP 279A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101636 ER PT J AU Alter, BP Joenje, H Oostra, AB Pals, G AF Alter, BP Joenje, H Oostra, AB Pals, G TI Fanconi's Anemia (FA): First diagnosis in an adult with head and neck cancer and hematopoletic somatic mosaicisin SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 Vrije Univ Amsterdam, Med Ctr, Dept Clin Genet & Human Genet, Amsterdam, Netherlands. NCI, Clin Genet Branch, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. RI Pals, Gerard/A-5198-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1649 BP 290A EP 290A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101698 ER PT J AU Liu, LL Su, BGY Nowicki, PT Wang, XT Liu, YS AF Liu, LL Su, BGY Nowicki, PT Wang, XT Liu, YS TI Triptolide inhibits the PI3-Kinase/Akt pathway and induces apoptosis of human tumor cells SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 03-06, 2003 CL SEATTLE, WA SP Pediat Acad Soc, Amer Pediat Soc, Soc Pediat Res, Ambulatory Pediat Assoc, Tulane Univ Hlth Sci Ctr, Ctr Continuing Educ C1 NIA, Lab Cellular & Mol Biol, Baltimore, MD 21224 USA. Columbus Childrens Res Inst, Ctr Cell & Vasc Biol, Columbus, OH USA. Childrens Res Inst, Ctr Dev Pharmacol & Toxicol, Columbus, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1706 BP 300A EP 300A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101755 ER PT J AU Raju, TNK AF Raju, TNK TI Dr Julius Hess and the ""electric-heated," "water-jacketed" incubator SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Pregnancy & Perinatol Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1778 BP 313A EP 313A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101844 ER PT J AU Gafni, RI Hazra, R Reynolds, JC Maldarelli, F Tullio, A DeCarlo, E Worrell, C Zuckerman, J Zeichner, S AF Gafni, RI Hazra, R Reynolds, JC Maldarelli, F Tullio, A DeCarlo, E Worrell, C Zuckerman, J Zeichner, S TI Decreased bone density (BMD) in HIV-infected children treated with tenofovir disoproxil fumarate (TDF)-containing HAART SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 HAMB, NCI, NIH, Bethesda, MD USA. NMD, CC, NIH, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1873 BP 329A EP 329A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101939 ER PT J AU Taylor, P Worrell, C Steinberg, SM Hazra, R Wood, LV Zwerski, S Zeichner, S AF Taylor, P Worrell, C Steinberg, SM Hazra, R Wood, LV Zwerski, S Zeichner, S TI Natural history of lipid abnormalities and fat redistribution in HIV-infected children receiving long-term protease inhibitor-containing HAART regimens SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 HAMB, NCI, NIH, Bethesda, MD USA. NMD, CC, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1872 BP 329A EP 329A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101938 ER PT J AU Mirochnick, M Capparelli, E Blanchard, S Shetty, AK Coovadia, HM Wells, J Dilraj, A Mateta, P Mofenson, L Jones, SA AF Mirochnick, M Capparelli, E Blanchard, S Shetty, AK Coovadia, HM Wells, J Dilraj, A Mateta, P Mofenson, L Jones, SA TI Population pharmacokinetics (pop pk) of low dose nevirapine (NVP) for prevention of breast milk HIV transmission in infants from birth through 6 months of age SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Boston Univ, Sch Med, Boston, MA 02118 USA. UCSD, San Diego, CA USA. SDAC, Boston, MA USA. Stanford Univ, Sch Med, Palo Alto, CA 94304 USA. Univ Natal, ZA-4001 Durban, South Africa. Zimbabwe AIDS Prevent Project, Harare, Zimbabwe. Univ Zimbabwe, Harare, Zimbabwe. MRC, Durban, South Africa. NICHD, NIH, Bethesda, MD USA. NIAID, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1876 BP 330A EP 330A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101942 ER PT J AU Bonville, CA Rosenberg, HE Domachowske, JB AF Bonville, CA Rosenberg, HE Domachowske, JB TI Correlation of gene microarray expression data with plethysmographic analysis during severe pneumovirus infection in mice SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 SUNY Upstate Med Univ, Syracuse, NY USA. NIAID, Eosinophil Biol Unit, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1907 BP 335A EP 335A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101973 ER PT J AU Zaoutis, TE Chu, JH Argon, J Walsh, TJ Feudtner, C AF Zaoutis, TE Chu, JH Argon, J Walsh, TJ Feudtner, C TI Candidemia in hospitalized children: Epidemiology and outcomes in the United States, 2000 SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Childrens Hosp Philadelphia, Div Infect Dis, Philadelphia, PA 19104 USA. Childrens Hosp Philadelphia, Div Gen Pediat, Pediat Generalist Res Grp, Philadelphia, PA 19104 USA. NCI, Immunocompromised Host Sect, Pediat Oncol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 1916 BP 337A EP 337A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591101982 ER PT J AU Bada, HS Bauer, C Shankaran, S Lester, B LaGasse, L Das, A Hammond, J Gard, C Wright, L Smeriglio, V AF Bada, HS Bauer, C Shankaran, S Lester, B LaGasse, L Das, A Hammond, J Gard, C Wright, L Smeriglio, V TI Long-term effects of prenatal exposure to cocaine, opiates and other drugs on childhood behavior problems: The maternal lifestyle study (MLS) SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, NIH, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2121 BP 373A EP 373A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102187 ER PT J AU Johnson, YR Shankaran, S Nadya, S Kazzi, J Chen, XG Lua, J AF Johnson, YR Shankaran, S Nadya, S Kazzi, J Chen, XG Lua, J TI A comparison in developmental outcomes among ELBW infants with BPD discharged from the NICU with and without home oxygen therapy SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, Bethesda, MD USA. Wayne State Univ, Detroit, MI USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2132 BP 375A EP 375A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102198 ER PT J AU Llanos, AR Mena, P Yuhong, L Salem, N Uauy, R AF Llanos, AR Mena, P Yuhong, L Salem, N Uauy, R TI Comparison of the metabolism of 18-and 20-carbon essential fatty acids in human neonates SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ Chile, INTA, Santiago, Chile. NIAAA, Lab Membrane Biochem & Biophys, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2167 BP 382A EP 383A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102242 ER PT J AU Llanos, AR Yuhong, L Pawlosky, R Mena, P Norman, S Uauy, R AF Llanos, AR Yuhong, L Pawlosky, R Mena, P Norman, S Uauy, R TI Determination of in vivo kinetic rate parameters of n-6 fatty acid metabolism in full term infants using stable isotopes SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ Chile, INTA, Santiago, Chile. NIAAA, Rockville, MD 20852 USA. NEORED Neonatal Network, Santiago, Chile. NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2177 BP 383A EP 383A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102243 ER PT J AU Lin, FYC Philips, JB Azimi, P Regan, J Rhoads, GG Clark, P Troendle, J Moyer, P Weisman, LE AF Lin, FYC Philips, JB Azimi, P Regan, J Rhoads, GG Clark, P Troendle, J Moyer, P Weisman, LE TI Heavy carriage of group B streptococci at birth is associated with neonatal morbidity other than sepsis SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. Univ Med & Dent New Jersey, Dept Environm & Community Med, Piscataway, NJ 08854 USA. Univ Florida, Div Maternal Fetal Med, Gainesville, FL USA. Columbia Univ, Dept Pediat, New York, NY 10027 USA. Childrens Hosp, Med Ctr N Calif, Dept Pediat, Oakland, CA 94609 USA. Univ Alabama, Dept Pediat, Birmingham, AL USA. NICHHD, Div Epidemiol Stat & Prevent Res, NIH, DHHS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2250 BP 396A EP 396A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102316 ER PT J AU Panigrahi, P Satpathy, R Panda, P Pradhan, L Misra, P Jayakar, A Pote, M Shinkre, N Chaudhry, R Gewolb, I Wright, L Poole, K Morris, JG Parida, S Johnson, J AF Panigrahi, P Satpathy, R Panda, P Pradhan, L Misra, P Jayakar, A Pote, M Shinkre, N Chaudhry, R Gewolb, I Wright, L Poole, K Morris, JG Parida, S Johnson, J TI Neonatal sepsisin India: Microbiology of infection and role of bacterial colonization SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ Maryland, Baltimore, MD 21201 USA. AIIMS, New Delhi, India. Capital Hosp Bhubaneswar, IGH Rourkela, Bhubaneswar, Orissa, India. Nair Hosp Mumbai, Bombay, Maharashtra, India. NICHD, Bethesda, MD 20892 USA. RTI Intl NC, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2276 BP 400A EP 401A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102342 ER PT J AU Wadhawan, R Perritt, R Poole, K Higgins, R Oh, W AF Wadhawan, R Perritt, R Poole, K Higgins, R Oh, W TI Lack of early postnatal weight loss is not associated with an increased risk of BPD in small for gestational age extremely low birth weight infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatul Res Network, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2490 BP 438A EP 438A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102555 ER PT J AU Watterberg, KL AF Watterberg, KL CA PROPHET Study Grp TI Prophylaxis of early adrenal insufficiency (Al) to prevent BPD: Multicenter trial SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ New Mexico, PROPHET Study Grp, NICHD, Albuquerque, NM 87131 USA. NR 0 TC 4 Z9 5 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2642 BP 465A EP 466A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102707 ER PT J AU Garmany, TH Hamavs, A Moxley, MA White, FV Wert, SE Whitsett, JA Dean, M Nogee, M AF Garmany, TH Hamavs, A Moxley, MA White, FV Wert, SE Whitsett, JA Dean, M Nogee, M TI Surfactant composition and function in patients with ABCA3 mutations SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Washington Univ, St Louis, MO USA. St Louis Univ, St Louis, MO 63103 USA. Johns Hopkins Univ, Baltimore, MD USA. Childrens Hosp, Med Ctr, Cincinnati, OH 45229 USA. Natl Canc Inst, Human Genet Sect, Frederick, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2662 BP 469A EP 469A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102727 ER PT J AU Hunter, CJ Dejam, A Blood, AB Hopper, AO Schechter, AN Power, GG Gladwin, MT AF Hunter, CJ Dejam, A Blood, AB Hopper, AO Schechter, AN Power, GG Gladwin, MT TI Nebulized nitrite selectively reverses hypoxic pulmonary vasoconstriction in newborn lambs SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NIDDK, Natl Inst Hlth, Bethesda, MD USA. Loma Linda Univ, Sch Med, Ctr Perinatal Biol, Loma Linda, CA 92350 USA. Loma Linda Univ, Sch Med, Dept Pediat, Loma Linda, CA 92350 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2676 BP 472A EP 472A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102741 ER PT J AU Wadhawan, R Vohr, B Perritt, R Poole, K Oh, W AF Wadhawan, R Vohr, B Perritt, R Poole, K Oh, W TI Association of twin gestation with an adverse neuro-developmental outcome at 18-22 months of corrected age in extremely low birth weight infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD Neonatal Res Network, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2714 BP 478A EP 478A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102779 ER PT J AU Lee, BH Stoll, BJ McDonald, SA Higgins, RD AF Lee, BH Stoll, BJ McDonald, SA Higgins, RD TI Adverse neonatal outcomes associated with antenatal dexamethasone (AD) versus antenatal betamethasone (AB) in very low birth weight (VLBW) infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Emory Univ, Atlanta, GA 30322 USA. Res Triangle Inst, Res Triangle Pk, NC 27709 USA. NICHD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2720 BP 479A EP 480A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102785 ER PT J AU Hillman, LS Day, LS Hoffman, HJ Stockbauer, JV Land, GH AF Hillman, LS Day, LS Hoffman, HJ Stockbauer, JV Land, GH TI 10-year follow-up of very low birthweight infants and controls: A population-based study SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ Missouri, Columbia, MO USA. NIDCD, NIH, Bethesda, MD USA. Dept Hlth, Jefferson City, MO USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2724 BP 480A EP 480A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102789 ER PT J AU Bradt, P O'Shea, M Gard, C Tyson, J Vohr, B Wright, L AF Bradt, P O'Shea, M Gard, C Tyson, J Vohr, B Wright, L TI Developing simpler methods to diagnose cerebral palsy: Use of functional motor skills in extremely low birth weight (ELBW) infants at 18-22 months SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Univ Texas, Houston Hlth Sci Ctr, Houston, TX USA. Wake Forest Univ, Winston Salem, NC 27109 USA. Res Triangle Inst, Div Stat Res, Rockville, MD USA. Brown Univ, Providence, RI 02912 USA. NICHD, NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2738 BP 483A EP 483A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102803 ER PT J AU Ambalavanan, N Tyson, JE Kennedy, KA Hansen, N Vohr, BR Wright, LL Carlo, WA AF Ambalavanan, N Tyson, JE Kennedy, KA Hansen, N Vohr, BR Wright, LL Carlo, WA CA NICHD Neonatal Res Network TI Does vitamin A supplementation affect long-term neurodevelopment in extremely low birth weight (ELBW) infants? SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2860 BP 504A EP 505A PN 2 PG 2 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102925 ER PT J AU Duara, S Poindexter, BB Saha, S Ehrenkranz, RA Higgins, RD Poole, K AF Duara, S Poindexter, BB Saha, S Ehrenkranz, RA Higgins, RD Poole, K CA NICHD Neonatal Res Network TI Human milk as protection against bronchopulmonary dysplasia (BPD) in extremely low birth weight infants (ELBW) SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 1 U1 1 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 2985 BP 527A EP 527A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591103066 ER PT J AU Wadhawan, R Ehrenkranz, R Finer, N Perritt, R Poole, K Oh, W AF Wadhawan, R Ehrenkranz, R Finer, N Perritt, R Poole, K Oh, W TI Oxygenation index overestimates the degree of lung disease severity in infants on high frequency ventilation SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 3182 BP 561A EP 561A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591103261 ER PT J AU Shankaran, S Laptook, AR Ehrenkranz, RA Donovan, EA Fanaroff, AA Tyson, IE McDonald, SA Poole, K Wright, LL Goldberg, R AF Shankaran, S Laptook, AR Ehrenkranz, RA Donovan, EA Fanaroff, AA Tyson, IE McDonald, SA Poole, K Wright, LL Goldberg, R TI Safety of whole body hypothermia for hypoxic-ischemic encephalopathy(HIE) SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 3306 BP 582A EP 582A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591103383 ER PT J AU El-Mohandes, AAE El-Khorazaty, N Murray, K Sharps, P Kiely, M AF El-Mohandes, AAE El-Khorazaty, N Murray, K Sharps, P Kiely, M TI Factors associated with intimate partner violence during pregnancy in minority populations SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 George Washington Univ, Prevent & Community Hlth, Sch Publ Hlth & Hlth Serv, Washington, DC USA. Res Triangle Inst, Rockville, MD USA. Johns Hopkins Univ, Sch Nursing, Baltimore, MD USA. NICHHD, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 3457 BP 609A EP 609A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591103534 ER PT J AU McKinney, RE Cruz, MLS Powell, C Hughes, M Oleske, JM Winter, H Elgie, C Perdue, L Wolf, D Ortiz-Pujols, S Calles, NR McNamara, J Moye, J AF McKinney, RE Cruz, MLS Powell, C Hughes, M Oleske, JM Winter, H Elgie, C Perdue, L Wolf, D Ortiz-Pujols, S Calles, NR McNamara, J Moye, J TI International formula-based nutritional intervention for infants born to HIV-infected women SO PEDIATRIC RESEARCH LA English DT Meeting Abstract CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04, 2004 CL San Francisco, CA SP Pediatr Acad Soc C1 Duke Univ, Ctr Med, Durham, NC USA. Hosp Servidores Estado, Rio De Janeiro, Brazil. Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. Univ Newark, Newark, NJ USA. Massachusetts Gen Hosp, Boston, MA 02114 USA. Tech Res Fndn, Amherst, NY USA. DHHS, NIH, Bethesda, MD USA. Ross Prod Div, Columbus, OH USA. Soc Sci Syst, Silver Spring, MD USA. Texas Childrens Hosp, Houston, TX 77030 USA. NJCHD, DHHS, NIH, Bethesda, MD USA. RI Oleske, James/C-1951-2016 OI Oleske, James/0000-0003-2305-5605 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 2004 VL 55 IS 4 SU S MA 16 BP ER43 EP ER43 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 808TJ UT WOS:000220591102951 ER PT J AU Jensen, PS Arnold, LE Severe, JB Vitiello, B Hoagwood, K AF Jensen, PS Arnold, LE Severe, JB Vitiello, B Hoagwood, K CA MTA Cooperative Grp TI National Institute of Mental Health Multimodal Treatment Study of ADHD follow-up: 24-month outcomes of treatment strategies for attention-deficit/hyperactivity disorder SO PEDIATRICS LA English DT Article DE ADHD; attention deficit; hyperactivity; stimulant medication; behavior therapy ID DEFICIT HYPERACTIVITY DISORDER; COLLABORATIVE RESEARCH-PROGRAM; CLINICAL-SIGNIFICANCE; MTA; CHILDREN; PSYCHOTHERAPY; PERFORMANCE; SYMPTOMS; MODELS; TRIAL AB Objective. In the Multimodal Treatment Study of ADHD (MTA), the effects of medication management (MedMgt) and behavior modification therapy (Beh) and their combination ( Comb) and usual community comparison ( CC) in the treatment of attention-deficit/ hyperactivity disorder ( ADHD) differed at the 14-month assessment as a result of superiority of the MTA MedMgt strategy ( Comb or MedMgt) over Beh and CC and modest additional benefits of Comb over MedMgt alone. Here we evaluate the persistence of these beneficial effects 10 months beyond the 14 months of intensive intervention. Methods. Of 579 children who entered the study, 540 (93%) participated in the first follow-up 10 months after the end of treatment. Mixed-effects regression models explored possible persisting effects of the MTA medication strategy, the incremental benefits of Comb over MedMgt alone, and the possible superiority of Beh over CC on 5 effectiveness and 4 service use domains. Results. The MTA medication strategy showed persisting significant superiority over Beh and CC for ADHD and oppositional-defiant symptoms at 24 months, although not as great as at 14 months. Significant additional benefits of Comb over MedMgt and of Beh over CC were not found. The groups differed significantly in mean dose ( methylphenidate equivalents 30.4, 37.5, 25.7, and 24.0 mg/day, respectively). Continuing medication use partly mediated the persisting superiority of Comb and MedMgt. Conclusion. The benefits of intensive MedMgt for ADHD extend 10 months beyond the intensive treatment phase only in symptom domains and diminish over time. C1 NIMH, MTA Cooperat Grp, Bethesda, MD 20892 USA. RP Arnold, LE (reprint author), Columbia Univ, New York State Psychiat Inst, Dept Child Psychiat, Ctr Advancement Child & Adolescent Mental Hlth, Unit 78,1051 Riverside Dr, New York, NY 10032 USA. EM arnold.6@osu.edu OI Jensen, Peter/0000-0003-2387-0650 NR 31 TC 241 Z9 245 U1 6 U2 39 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD APR 1 PY 2004 VL 113 IS 4 BP 754 EP 761 PG 8 WC Pediatrics SC Pediatrics GA 808RB UT WOS:000220585100010 ER PT J AU Vohr, BR Wright, LL Dusick, AM Perritt, R Poole, WK Tyson, JE Steichen, JJ Bauer, CR Wilson-Costello, DE Mayes, LC AF Vohr, BR Wright, LL Dusick, AM Perritt, R Poole, WK Tyson, JE Steichen, JJ Bauer, CR Wilson-Costello, DE Mayes, LC CA Neonatal Res Network TI Center differences and outcomes of extremely low birth weight infants SO PEDIATRICS LA English DT Article DE extremely low birth weight; center differences; neurologic outcome; developmental outcome; cerebral palsy; Bayley; follow-up studies ID NEONATAL INTENSIVE-CARE; NATIONAL-INSTITUTE; CHILD-HEALTH; UNITS; GLUCOCORTICOIDS; NEWBORNS; SEVERITY; ILLNESS; NETWORK; RISK AB Objective. Previous multicenter studies have shown significant center differences in neonatal characteristics and morbidities. This study evaluated center differences in outcome at 18 to 22 months among extremely low birth weight (ELBW; 401- 1000 g) infants after adjusting for demographics and antenatal interventions, and it identified neonatal interventions associated with outcome differences. Methods. We assessed the outcome of 2478 liveborn infants who were admitted in 1993 and 1994 to the 12 centers of the Neonatal Research Network of the National Institute of Child Health and Human Development; 1483 (60%) infants survived to 18 to 22 months, and 1151 (78%) had comprehensive evaluations. Logistic regression analyses were performed to identify center differences and the association of 4 neonatal interventions - active resuscitation, postnatal steroids, ventilator treatment for less than or equal to 27 days, and full enteral feedings less than or equal to 24 days - with adverse outcomes ( cerebral palsy, low Bayley scores, and neurodevelopmental impairment [NDI]), after adjusting for demographics and antenatal interventions. Results. Using bivariate analyses, significant center differences were identified for mortality, antenatal and postnatal interventions, social and environmental variables, neonatal morbidities, and neurodevelopmental outcomes for the 12 centers. After adjustment for maternal and infant demographics and antenatal interventions, the percentage of ELBW infants who had died or had NDI at 18 to 22 months ranged from 52% to 85%. Active resuscitation and postnatal steroids were associated with increases of NDI of 11.8% and 19.3%, whereas shorter ventilation support and shorter time to achieve full enteral feeds were associated with decreases in NDI of 20.7% and 17.3%, respectively. Conclusion. There are large and disturbing differences among centers in outcomes at 18 to 22 months after adjusting for demographic and antenatal interventions. Center differences in postnatal interventions associated with differences in outcome can provide hypotheses for testing in clinical trials to improve outcome. C1 Brown Univ, Women & Infants Hosp, Providence, RI 02905 USA. NICHHD, Bethesda, MD 20892 USA. Indiana Univ, Indianapolis, IN 46204 USA. Res Triangle Inst, Res Triangle Pk, NC 27709 USA. Univ Texas, SW Med Ctr, Dallas, TX USA. Univ Cincinnati, Cincinnati, OH USA. Univ Miami, Miami, FL 33152 USA. Case Western Reserve Univ, Cleveland, OH 44106 USA. Yale Univ, New Haven, CT USA. RP Vohr, BR (reprint author), Brown Univ, Women & Infants Hosp, 101 Dudley St, Providence, RI 02905 USA. EM betty_vohr@brown.edu FU NCRR NIH HHS [M01 RR 00070, M01 RR 00750, M01 RR 00997, M01 RR 06022, M01 RR 08084]; NICHD NIH HHS [U10 HD27881, U01 HD36790, U10 HD21364, U10 HD21373, U10 HD21385, U10 HD21397, U10 HD21415, U10 HD27851, U10 HD27853, U10 HD27856, U10 HD27871, U10 HD27880, U10 HD27904] NR 28 TC 155 Z9 167 U1 0 U2 3 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD APR 1 PY 2004 VL 113 IS 4 BP 781 EP 789 DI 10.1542/peds.113.4.781 PG 9 WC Pediatrics SC Pediatrics GA 808RB UT WOS:000220585100014 PM 15060228 ER PT J AU Swedo, SE Leonard, HL Rapoport, JL AF Swedo, SE Leonard, HL Rapoport, JL TI The pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection (PANDAS) subgroup: Separating fact from fiction SO PEDIATRICS LA English DT Editorial Material ID OBSESSIVE-COMPULSIVE DISORDER; CLINICAL DESCRIPTION; TOURETTES-SYNDROME; RHEUMATIC-FEVER; PLASMA-EXCHANGE; CHILDREN; ADOLESCENTS; CHILDHOOD; EXACERBATIONS; CHOREA C1 NIMH, Pediat & Dev Neuropsychiat Branch, Intramural Res Program, Bethesda, MD 20892 USA. Brown Univ, Div Child Psychiat, Providence, RI 02912 USA. NIMH, Child Psychiat Branch, Intramural Res Program, Bethesda, MD 20892 USA. RP Swedo, SE (reprint author), NIMH, Pediat & Dev Neuropsychiat Branch, Intramural Res Program, 10 Ctr Dr,MSC 1255, Bethesda, MD 20892 USA. EM swedos@mail.nih.gov NR 30 TC 86 Z9 93 U1 1 U2 9 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD APR 1 PY 2004 VL 113 IS 4 BP 907 EP 911 DI 10.1542/peds.113.4.907 PG 5 WC Pediatrics SC Pediatrics GA 808RB UT WOS:000220585100028 PM 15060242 ER PT J AU Miller, RW AF Miller, RW TI How environmental hazards in childhood have been discovered: Carcinogens, teratogens, neurotoxicants, and others SO PEDIATRICS LA English DT Article DE case reports; case series; epidemiology; disease clusters; causality ID COLA-COLORED BABIES; MALIGNANT-MELANOMA; UMBILICAL CORDS; UNITED-STATES; RECENT TRENDS; IN-UTERO; CANCER; EXPOSURE; LEUKEMIA; IRRADIATION AB Review of the literature reveals that environmental hazards cause adverse health effects that include sterility, infertility, embryotoxicity, low birth weight, skin lesions, neurodevelopmental defects, immunologic disorders, cancer, and fear of late effects. They have been identified mostly by astute practitioners but also by a bacteriologist, an animal experimentalist, 5 factory workers in childless marriages, and a tipsy by-stander in an economically impoverished area of Baltimore. Dust on a parent's work clothes has transported a hazard at work to a hazard at home (lead, asbestos, and chlordecone). Causality is established by showing a dose-response effect and reproducing the effect in studies of other exposed groups or by using another epidemiologic method, eg, prospective instead of retrospective study. Also, the findings should be biologically plausible and not attributable to a concomitant variable such as cigarette smoking. Contrary to front-page newspaper headlines, incidence rates for childhood leukemia are not rising. Preserving specimens for future studies has been valuable: blood from people who were exposed to dioxin in Seveso, Italy; mummified umbilical cords containing methyl mercury at Minamata Bay, Japan; and Guthrie dried blood spots to screen retrospectively for 43 genetic disorders and a specific prenatal cytogenetic abnormality in some children with 1 form of leukemia. Recommendations are given for enhancing interest in environmental hazards and their discovery by clinicians. C1 NCI, Clin Genet Branch, Bethesda, MD 20892 USA. RP Miller, RW (reprint author), 5601 Alta Vista Rd, Bethesda, MD 20817 USA. EM millerro@mail.nih.gov NR 69 TC 13 Z9 14 U1 1 U2 3 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD APR 1 PY 2004 VL 113 IS 4 SU S BP 945 EP 951 PG 7 WC Pediatrics SC Pediatrics GA 808RC UT WOS:000220585200003 PM 15060186 ER PT J AU Lang, DM Butz, AM Duggan, AK Serwint, JR AF Lang, DM Butz, AM Duggan, AK Serwint, JR TI Physical activity in urban school-aged children with asthma SO PEDIATRICS LA English DT Article; Proceedings Paper CT Annual Meeting of the Pediatric-Academic-Societies CY MAY 04-07, 2002 CL BALTIMORE, MD SP Pediat Acad Soc DE asthma; child; exercise; health behavior; physical activity ID EXERCISE-INDUCED ASTHMA; CHILDHOOD ASTHMA; FITNESS; IMPACT; HEALTH; PREVALENCE; CAPACITY; OBESITY; BELIEFS; TRENDS AB Objectives. To compare the physical activity levels of children with and without asthma and evaluate predictors of activity level in children with asthma. Methods. Parents of 137 children with asthma and 106 controls 6 to 12 years old who attended an urban primary care pediatric clinic were interviewed by telephone. A structured survey evaluated 1 day's total activity and the number of days active in a typical week; asthma characteristics and treatment; physician advice; opportunities for physical activity; and caregiver beliefs about physical activity. The activity levels of children with and without asthma were compared. Predictors of activity level of children with asthma were evaluated. Results. Children with asthma were less active than their peers. The mean amount of daily activity differed by group: 116 ( asthma) vs 146 ( nonasthma) minutes; 21% ( asthma) vs 9% ( nonasthma) were active < 30 minutes/ day; and 23% ( asthma) vs 11% ( nonasthma) were active < 3 days/ week. Among children with asthma, disease severity and parental beliefs regarding exercise and asthma predicted activity level. Children with moderate or severe persistent asthma were more likely to be active < 30 minutes/ day ( odds ratio: 3.0; confidence interval: 1.2 - 7.5), and children whose parents believed exercise could improve asthma were more likely to be highly active &GE; 120 minutes/ day ( odds ratio: 2.5; confidence interval: 1.2 - 5.4). Conclusions. Disease severity and parental health beliefs contribute to the lower activity level of children with asthma. Pediatricians should evaluate exercise level as an indicator of disease control and address exercise and its benefits with patients and caregivers to help achieve the goal of normal physical activity in children with asthma. C1 NIH, Warren Grant Magnuson Clin Ctr, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA. RP Lang, DM (reprint author), 10 Ctr Dr,Rm 6C410,MSC 1625, Bethesda, MD 20892 USA. EM dlang@mail.cc.nih.gov FU PHS HHS [5 T32 HP 10004] NR 32 TC 63 Z9 66 U1 2 U2 10 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD APR 1 PY 2004 VL 113 IS 4 BP E341 EP E346 DI 10.1542/peds.113.4.e341 PG 6 WC Pediatrics SC Pediatrics GA 808RB UT WOS:000220585100052 PM 15060265 ER PT J AU Han, ZZ Simpson, JT Fivash, MJ Fisher, R Mori, T AF Han, ZZ Simpson, JT Fivash, MJ Fisher, R Mori, T TI Identification and characterization of peptides that bind to cyanovirin-N, a potent human immunodeficiency virus-inactivating protein SO PEPTIDES LA English DT Article DE cyanovirin-N; CV-N peptide; phage display; library; HIV; gp120 ID HIGH-MANNOSE OLIGOSACCHARIDES; GP120 ENVELOPE GLYCOPROTEIN; MAJOR COAT PROTEIN; PHAGE DISPLAY; FILAMENTOUS BACTERIOPHAGE; ENZYME-INHIBITORS; ESCHERICHIA-COLI; LIBRARIES; AFFINITY; FUSION AB Cyanovirin-N (CV-N) exerts a potent human immunodeficiency virus (HIV)-inactivating activity against diverse strains of HIV by binding to the viral surface envelope glycoprotein up 120 and blocking its essential interactions with cellular receptors. Based on previous thermodynamic analyses, it has been speculated that discrete protein-protein interactions might play an important ancillary role in the CV-N/gp120 binding event, in addition to the interactions of CV-N with specific oligosaccharides present on gp120. Here, we report the identification and characterization of CV-N-binding peptides, which were isolated by screening of M13 phage-displayed peptide libraries. After performing three rounds of biopanning of the libraries against biotinylated CV-N, a CV-N-binding motif, X3CX6(W/F)(Y/F)CX2(Y/F), was evident. A vector was designed to express CV-N-binding peptides as a fusion with thioredoxin (Trx) containing a penta-His affinity tag. The CV-N-binding peptides fused with His-tagged Trx inhibited binding of the corresponding peptide-bearing phages to CV-N, confirming that the peptides possessed CV-N-binding activity. Optical biosensor binding studies showed that the one of the CV-N-binding peptide, TN10-1, bound to CV-N with a K-D value of 1.9 muM. The results of alanine scanning mutagenesis of the peptide showed that aromatic residues at positions 11, 12, and 16, as well as the conformational structure of the peptide secured by a disulfide bond, were important for the binding interactions. A series of competitive binding assays confirmed that up 120 inhibited CV-N binding of the corresponding peptide-bearing phages, and suggested that TN10-1 peptides were mimicking the protein component of gp120 rather than mimicking specific oligosaccharides present on gp120. (C) 2004 Elsevier Inc. All rights reserved. C1 NCI, Ctr Canc Res, Mol Targets Dev Program, Ft Detrick, MD 21702 USA. SAIC Frederick Inc, Res Technol Program, Prot Chem Lab, Ft Detrick, MD 21702 USA. NCI, Data Management Serv Inc, Ft Detrick, MD 21702 USA. RP Mori, T (reprint author), NCI, Ctr Canc Res, Mol Targets Dev Program, Ft Detrick, MD 21702 USA. EM mori@ncifcrf.gov NR 42 TC 3 Z9 7 U1 0 U2 3 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0196-9781 J9 PEPTIDES JI Peptides PD APR PY 2004 VL 25 IS 4 BP 551 EP 561 DI 10.1016/j.peptides.2004.02.018 PG 11 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy GA 829IA UT WOS:000222039800003 PM 15165709 ER PT J AU Pickworth, WB Lee, EM Abreu, ME Umbricht, A Preston, KL AF Pickworth, WB Lee, EM Abreu, ME Umbricht, A Preston, KL TI A laboratory study of hydromorphone and cyclazocine on smoking behavior in residential polydrug users SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE cyclazocine; hydromorphone; smoking; polydrug use; kappa opioid agonist ID SUSTAINED-RELEASE BUPROPION; FREELY MOVING RATS; CIGARETTE-SMOKING; NUCLEUS-ACCUMBENS; MORPHINE-LIKE; NICOTINE; DOPAMINE; NALTREXONE; OPIATE; DRUGS AB The effects of cyclazocine and hydromorphone on spontaneous and laboratory cigarette smoking were compared in a double-blind, placebo-controlled, crossover study. Participants (seven men, one woman) received oral doses of placebo, cyclazocine (0.2, 0.4, and 0.8 mg) and hydromorphone (5 and 15 mg) in a randomized order on experimental days. Spontaneous smoking was recorded during two intervals on the experimental days: a 3-h period 5-8 It after drug administration (Interval 1), and the rest of the day (Interval 2). Measures of smoking topography and subjective and physiologic effects of a single cigarette were obtained on the experimental days. Neither hydromorphone nor cyclazocine significantly changed spontaneous smoking when compared to the placebo condition; however, compared to hydromorphone (5 mg), cyclazocine (0.4 and 0.8 mg) decreased spontaneous smoking during Interval 1. Hydromorphone (5 and 15 mg) and cyclazocine (0.4 and 0.8 mg) diminished smoking-induced increases in heart rate. Compared to the placebo condition, cyclazocine (0.2 and 0.4 mg) reduced exhaled carbon monoxide (CO) boost, a measure of smoke exposure. Further studies of the effects of kappa opioid agonists on smoking behavior may lead to a better understanding of the role of opiates in smoking behavior. (C) 2004 Elsevier Inc. All rights reserved. C1 NIDA, Intraumural Res Program, Clin Pharmacol & Therapeut Branch, Baltimore, MD 21224 USA. RP Pickworth, WB (reprint author), NIDA, Intraumural Res Program, Clin Pharmacol & Therapeut Branch, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM wpickwo@intra.nida.nih.gov RI Preston, Kenzie/J-5830-2013 OI Preston, Kenzie/0000-0003-0603-2479 NR 32 TC 5 Z9 5 U1 1 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD APR PY 2004 VL 77 IS 4 BP 711 EP 715 DI 10.1016/j.pbb.2004.01.022 PG 5 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA 816XP UT WOS:000221142900007 PM 15099916 ER PT J AU Matsumoto, RR Potelleret, FH Mack, A Pouw, B Zhang, Y Bowen, WD AF Matsumoto, RR Potelleret, FH Mack, A Pouw, B Zhang, Y Bowen, WD TI Structure activity comparison of YZ-069, a novel sigma ligand, and four analogs in receptor binding and behavioral studies SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE cocaine; sigma receptor; convulsions ID COCAINE-INDUCED CONVULSIONS; BIOLOGICAL EVALUATION; INDUCED TOXICITY; RAT-BRAIN; AFFINITY; CLONING; MICE; IDENTIFICATION; ATTENUATE; SITE AB Earlier studies show that antagonism of alpha receptors using high to moderate affinity compounds or antisense oligodeoxynucleotides targeting the ol subtype significantly attenuates the behavioral effects of cocaine in mice. In this study, the novel sigma receptor ligand YZ-069 [N-phenylpropyl-N-1 -(3,4-dichlorophenethyl)piperazine] and four analogs (representing nitrophenyl and methoxyphenyl derivatives) were evaluated in receptor binding and behavioral studies to further delineate structural features that convey favorable anticocaine actions. In receptor binding studies, all of the compounds had low nanomolar affinities for sigma(1) and sigma2 receptors but only micromolar affinities for monoamine transporters. Consistent with the favorable affinities of the compounds for sigma receptors, they also significantly attenuated cocane-induced convulsions in mice. The compounds with the 3,4-dichlorophenyl and methoxyphenyl substitutions provided better protection against cocaine-induced convulsions than the nitrophenyl derivative. This is consistent with the reduced lipophilicity of the nitro substitution, which would reduce its ability to cross the blood-brain barrier. The position of the substituent on the phenyl ring had no significant effect on binding affinity or behavioral protective actions. Together with earlier studies, the data suggest that favorable features of sigma receptor ligands with anticocaine actions include high affinity for brain (T receptors, antagonistic actions at the receptor, and lipophilicity to facilitate crossing the blood-brain barrier. (C) 2004 Elsevier Inc. All rights reserved. C1 Univ Oklahoma, Hlth Sci Ctr, Dept Pharmaceut Sci, Oklahoma City, OK 73190 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. RP Matsumoto, RR (reprint author), Univ Oklahoma, Hlth Sci Ctr, Dept Pharmaceut Sci, POB 26901, Oklahoma City, OK 73190 USA. EM rae-matsumoto@ouhsc.edu FU NIDA NIH HHS [DA11979] NR 39 TC 14 Z9 15 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD APR PY 2004 VL 77 IS 4 BP 775 EP 781 DI 10.1016/j.pbb.2004.01.014 PG 7 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA 816XP UT WOS:000221142900014 PM 15099923 ER PT J AU Blech-Hermoni, Y Kiyatkin, EA AF Blech-Hermoni, Y Kiyatkin, EA TI State-dependent action of cocaine on brain temperature and movement activity: implications for movement sensitization SO PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR LA English DT Article DE cocaine; behavioral sensitization; brain temperature; neural activation; rats ID INDUCED BEHAVIORAL SENSITIZATION; POSITRON-EMISSION-TOMOGRAPHY; CEREBRAL BLOOD-FLOW; INCENTIVE-SENSITIZATION; GLUCOSE-UTILIZATION; DOPAMINE; RATS; AMPHETAMINE; ENVIRONMENT; PREDICTION AB Because neural activity is highly energy consuming and heat producing, brain temperature offers a reliable, real-time measure of an animal's activity state and its changes induced by environmental and drug challenges. Therefore, it allows evaluation of the activity state of an animal preceding drug administration and its relation to subsequent drug-induced neural effects. This approach was used to explore the state dependency of cocaine's effects. Brain and body temperatures, as well as locomotion were measured simultaneously in rats during repeated, daily administration of cocaine (15 mg/kg ip, daily for 5 days) under different experimental conditions. The drug was administered via (a) a chronically implanted catheter in quiet resting conditions, (b) an injection made under quiet rest or (c) an injection under activated conditions associated with placement in the cage. Although brain temperature and movement increased after cocaine administration in each condition, cocaine's action (evaluated as cocaine-saline difference for both parameters) was situational. Catheter-administered cocaine induced the strongest movement activation and robust, monophasic temperature increase, which remained relatively stable following each subsequent drug infusion. Cocaine injected during quiet and, especially, activated conditions, induced a weaker locomotor activation, while the temperature response (evaluated as drug-saline difference) had a biphasic pattern. Cocaine initially inhibited the temperature increases seen in saline-treated animals (0-20 min) and then induced a more prolonged hyperthermia, which was about twofold weaker than that seen after catheter-administered drug. Although movement activation gradually increased following repeated treatment in activated conditions, the magnitude of this sensitized motor response barely reached the levels induced by the initial cocaine administration via catheter. These data suggest that both the acute effects of cocaine in the brain and their chan e following repeated drug administration are dependent upon the 9 ongoing neural activity state of the animal. Cocaine's interaction with this activity state is a crucial factor determining the behavioral effects of this drug, including state-dependent motor sensitization. (C) 2004 Elsevier Inc. All rights reserved. C1 NIDA, Behav Neurosci Branch, Intramural Res Program, NIH,Dept Hlth & Human Serv, Baltimore, MD 21224 USA. RP Kiyatkin, EA (reprint author), NIDA, Behav Neurosci Branch, Intramural Res Program, NIH,Dept Hlth & Human Serv, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM ekiyatki@intra.nida.nih.gov NR 50 TC 8 Z9 8 U1 0 U2 1 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0091-3057 J9 PHARMACOL BIOCHEM BE JI Pharmacol. Biochem. Behav. PD APR PY 2004 VL 77 IS 4 BP 823 EP 837 DI 10.1016/j.pbb.2004.02.009 PG 15 WC Behavioral Sciences; Neurosciences; Pharmacology & Pharmacy SC Behavioral Sciences; Neurosciences & Neurology; Pharmacology & Pharmacy GA 816XP UT WOS:000221142900020 PM 15099929 ER PT J AU Gubbins, PO McConnell, SA Gurley, BJ Fincher, TK Franks, AM Williams, DK Penzak, SR Saccente, M AF Gubbins, PO McConnell, SA Gurley, BJ Fincher, TK Franks, AM Williams, DK Penzak, SR Saccente, M TI Influence of grapefruit juice on the systemic availability of itraconazole oral solution in healthy adult volunteers SO PHARMACOTHERAPY LA English DT Article DE itraconazole; grapefruit juice; enteric CYP3A4; absorption ID STEADY-STATE PHARMACOKINETICS; P-GLYCOPROTEIN; FOOD INTERACTION; DRUG; TRANSPORT; CAPSULES; BIOAVAILABILITY; FEXOFENADINE; FORMULATION; ABSORPTION AB Study Objective. To evaluate the effect of repeated ingestion of grapefruit juice on the systemic availability of itraconazole (ITZ) and hydroxyitraconazole (OHITZ) serum concentrations in subjects administered hydroxypropyl-beta-cyclodextrin-ITZ (HP-P-CD ITZ) oral solution. Design. Randomized, two-period, crossover study. Setting. College of pharmacy research unit. Subjects. Twenty healthy, adult volunteers (10 men, 10 women). Intervention. Subjects received 240 ml of regular-strength grapefruit juice from frozen concentrate or bottled purified water 3 times/day for 2 days. On the third day they received a single dose of HP-P-CD ITZ oral solution 200 mg (20 ml) with 240 ml of the beverage. Two hours after dosing they received another 240 ml of the beverage. Measurements and Main Results. Repeated blood samples were drawn for 72 hours after dosing. After a 14-day washout period, subjects were crossed over to the beverage they had not received previously and the above procedure was repeated. There was no difference in peak ITZ concentration (C-max) or time to C-max (T-max). Coadministration of grapefruit juice reduced OHITZ C-max nearly 10%, but this difference was not statistically significant. It produced a statistically significant increase in ITZ area under the concentration-time curves from 0-48 hours (AUC(0-48)) (17%) and from time zero extrapolated to infinity (AUC(0-infinity)) (19.5%). Apparent oral clearance of ITZ was significantly reduced (14%). Significant changes in OHITZ exposure were not observed; however, grapefruit juice coadministration produced statistically significant decreased mean OHITZ:ITZ AUC(0-48) and AUC(0-infinity) ratios. Grapefruit juice also decreased the mean OHITZ:ITZ Cmax ratio, but the difference was not statistically significant. Conclusion. Repeated grapefruit juice consumption moderately affects ITZ systemic availability in subjects administered HP-P-CD ITZ oral solution. Unlike previous findings with ITZ capsules, changes in the disposition of ITZ and CHITZ after repeated grapefruit juice consumption are consistent with grapefruit juice inhibition of intestinal cytochrome P450 3A4. C1 Univ Arkansas Med Sci, Coll Pharm, Dept Pharm Practice, Little Rock, AR 72205 USA. Univ Arkansas Med Sci, Coll Pharm, Dept Pharmaceut Sci, Little Rock, AR 72205 USA. Univ Arkansas Med Sci, Coll Publ Hlth, Little Rock, AR 72205 USA. Univ Arkansas Med Sci, Coll Med, Little Rock, AR 72205 USA. NIH, Ctr Clin, Dept Pharm, Clin Pharmacokinet Lab, Bethesda, MD 20892 USA. RP Gubbins, PO (reprint author), Univ Arkansas Med Sci, Coll Pharm, Dept Pharm Practice, 4301 W Markham St,Slot 522, Little Rock, AR 72205 USA. EM Gubbinspaulo@uams.edu NR 24 TC 20 Z9 21 U1 1 U2 2 PU PHARMACOTHERAPY PUBLICATIONS INC PI BOSTON PA NEW ENGLAND MEDICAL CENTER, 806, 750 WASHINGTON ST, BOSTON, MA 02111 USA SN 0277-0008 J9 PHARMACOTHERAPY JI Pharmacotherapy PD APR PY 2004 VL 24 IS 4 BP 460 EP 467 DI 10.1592/phco.24.5.460.33350 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 809GG UT WOS:000220624600005 PM 15098799 ER PT J AU Lamar, JE Cronin, CK Scott, LE AF Lamar, JE Cronin, CK Scott, LE TI A review of steps taken to create an international virtual laboratory at NASA Langley for aerodynamic prediction and comparison SO PROGRESS IN AEROSPACE SCIENCES LA English DT Review ID COMPUTATIONAL FLUID-DYNAMICS AB A review of the steps taken to establish an international virtual laboratory (VL) at the NASA Langley Research Center for aerodynamic prediction and comparison of flight data in the post-09/11/2001 cyber-terrorist environment is detailed here. The key features of the VL include an intuitive, web-based user interface for ease of access, a secure highspeed Internet connection between browser and server, a relational database architecture for data and information search, and a secure file-storage system. The detailed planning and handling of such issues as security, computer firewall access and legal protection of data are provided. Published by Elsevier Ltd. C1 NASA, Langley Res Ctr, Hampton, VA 23681 USA. NCI, ConITS Contract, Hampton, VA 23666 USA. RP Lamar, JE (reprint author), NASA, Langley Res Ctr, Hampton, VA 23681 USA. EM j.e.lamar@nasa.gov NR 29 TC 6 Z9 7 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0376-0421 J9 PROG AEROSP SCI JI Prog. Aeosp. Sci. PD APR PY 2004 VL 40 IS 3 BP 163 EP 172 DI 10.1016/j.paerosci.2004.03.002 PG 10 WC Engineering, Aerospace SC Engineering GA 830NQ UT WOS:000222129600003 ER PT J AU Duncan, T Chen, JM Friedman, FK Hyde, M Chie, L Pincus, MR AF Duncan, T Chen, JM Friedman, FK Hyde, M Chie, L Pincus, MR TI Comparison of molecular dynamics averaged structures for complexes of normal and oncogenic ras-p21 with SOS nucleotide exchange protein, containing computed conformations for three crystallographically undefined domains, suggests a potential role of these domains in ras signaling SO PROTEIN JOURNAL LA English DT Article DE average structure; effector domains; molecular dynamics; oncogenic ras-p21; SOS loop regions; SOS protein; wild-type ras-p21 ID INDUCED OOCYTE MATURATION; WILD-TYPE RAS-P21; EFFECTOR DOMAINS; 3-DIMENSIONAL STRUCTURES; SELECTIVE-INHIBITION; JUN PROTEINS; P21 PROTEIN; ACTIVATION; PEPTIDES; IDENTIFICATION AB ras-p21 protein binds to the son-of-sevenless (SOS) guanine nucleotide-exchange promoter that allows it to exchange GDP for GTP. Previously, we performed molecular dynamics calculations on oncogenic (Val 12-) and wild-type ras-p21 bound to SOS. By superimposing the average structures of these two complexes, we identified four domains (residues 631-641, 676-691, 718-729, and 994-1004) in SOS that change conformation and were candidates for being effector domains. These calculations were performed in the absence of three crystallographically undefined loops (i.e., residues 591-596, 654-675, and 742-751). We have now modeled these loops into the SOS structure and have re-performed the dynamics calculations. We find that all three loop domains undergo large changes in conformation that involve mostly changes in their positioning and not their individual conformations. We have also identified another potential effector domain (i.e., residues 980-989). Overall, our current results suggest that SOS interactions with oncogenic ras-p21 may enhance ras-p21 mitogenic signaling through prolonging its activation by maintaining its binding to GTP and by allowing its effector domains to interact with intracellular targets. C1 New York Harbor VA Med Ctr, Dept Pathol & Lab Med, Brooklyn, NY 11209 USA. SUNY Syracuse, Upstate Med Ctr, Dept Biochem, Syracuse, NY 13210 USA. Gilead Sci Inc, Struct Chem, Foster City, CA 94404 USA. NCI, Lab Metab, Bethesda, MD 20892 USA. Adv Comp Serv, De Witt, NY 13214 USA. SUNY, Downstate Med Ctr, Dept Pathol, Brooklyn, NY 11203 USA. RP Pincus, MR (reprint author), New York Harbor VA Med Ctr, Dept Pathol & Lab Med, Brooklyn, NY 11209 USA. EM matthew.pincus2@med.va.gov RI Friedman, Fred/D-4208-2016; OI Duncan, Thomas/0000-0002-6432-4958 FU NCI NIH HHS [CA 42500] NR 27 TC 5 Z9 5 U1 0 U2 0 PU KLUWER ACADEMIC/PLENUM PUBL PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1572-3887 J9 PROTEIN J JI Protein J. PD APR PY 2004 VL 23 IS 3 BP 217 EP 228 DI 10.1023/B:JOPC.0000026417.72621.1f PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 838YU UT WOS:000222751300005 PM 15200053 ER PT J AU Chie, L Friedman, FK Duncan, T Chen, JM Chung, D Pincus, M AF Chie, L Friedman, FK Duncan, T Chen, JM Chung, D Pincus, M TI Loop domain peptides from the SOS ras-guanine nucleotide exchange protein, identified from molecular dynamics calculations, strongly inhibit ras signaling SO PROTEIN JOURNAL LA English DT Article DE effector domain; SOS loop peptides; insulin; oncogenic ras; p21; oocyte maturation; SOS protein ID N-TERMINAL KINASE; ONCOGENIC RAS-P21; OOCYTE MATURATION; WILD-TYPE; INSULIN; ACTIVATION; TRANSDUCTION; INDUCTION; P21(RAS); SITE AB In the accompanying paper, we found, using molecular dynamics calculations, four domains of the ras-specific SOS guanine nucleotide exchange protein (residues 589-601, 654-675, 746-761, and 980-989) that differ markedly in conformation when SOS is complexed with either oncogenic (Val 12-) ras-p21 or wild-type ras-p21. Three of these domains contain three crystallographically undefined loops that we modeled in these calculations, and one is a newly identified non-loop domain containing SOS residues 980-989. We have now synthesized peptides corresponding to these four domains and find that all of them block Val 12-ras-p21-induced oocyte maturation. All of them also block insulin-induced oocyte maturation, but two of these peptides, corresponding to SOS residues 589-601 and 980-989, block oncogenic ras to a significantly greater extent. These results suggest that SOS contains domains, including the three loop domains, that are important for ras signaling and that several of these domains can activate different pathways specific to oncogenic or wild-type ras-p21. C1 New York Harbor VA Med Ctr, Dept Pathol & Lab Med, Brooklyn, NY 11209 USA. Long Isl Univ, Dept Biol, Brooklyn, NY 11201 USA. Long Isl Univ, Dept Chem, Brooklyn, NY 11201 USA. NCI, Lab Metab, Bethesda, MD 20892 USA. SUNY, Upstate Med Ctr, Dept Biochem, Syracuse, NY 13210 USA. Gilead Sci Inc, Struct Chem, Foster City, CA 94404 USA. SUNY, Downstate Med Ctr, Dept Pathol, Brooklyn, NY 11203 USA. RP Pincus, M (reprint author), New York Harbor VA Med Ctr, Dept Pathol & Lab Med, Brooklyn, NY 11209 USA. EM matthew.pincus2@med.va.gov RI Friedman, Fred/D-4208-2016; OI Duncan, Thomas/0000-0002-6432-4958 FU NCI NIH HHS [CA 42500] NR 20 TC 5 Z9 5 U1 0 U2 0 PU KLUWER ACADEMIC/PLENUM PUBL PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1572-3887 J9 PROTEIN J JI Protein J. PD APR PY 2004 VL 23 IS 3 BP 229 EP 234 DI 10.1023/B:JOPC.0000026418.82786.5e PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 838YU UT WOS:000222751300006 PM 15200054 ER PT J AU Chie, L Adler, V Friedman, FK Chung, D Pincus, MR AF Chie, L Adler, V Friedman, FK Chung, D Pincus, MR TI An effector peptide from glutathione-S-transferase-pi strongly and selectively blocks mitotic signaling by oncogenic ras-p21 SO PROTEIN JOURNAL LA English DT Article DE glutathione-S-transferase-pi; GST-pi peptide 34-50; inhibition; jun; jun-N-terminal kinase; oncogenic ras-p21; oocyte maturation ID JUN PROTEINS; RAS PROTEINS; KINASE; ACTIVATION; INHIBITION; P21(RAS); INSULIN; JNK AB Oncogenic ras-p21 directly activates jun-N-terminal kinase (JNK) and its substrate, jun as a unique step on its mitogenic signal transduction pathway. This activation is blocked by the specific JNK jun inhibitor, glutathione-S-transferase-pi (GST-pi). Four domains of GST-pi have been implicated in this regulatory function: 34-50, 99-121, 165-182, and 194-201. The 34-50 domain is unique in that it does not affect GST-pi binding to JNK jun but blocks jun phosphorylation by JNK. We now find that it completely blocks oncogenic (Val 12-) ras-p21-induced oocyte maturation but has no effect on insulin-induced oocyte maturation. Because the latter process requires activation of wild-type ras-p21, this peptide appears to be specific for inhibiting only the oncogenic form of ras-p21, suggesting its use in treating ras-induced tumors. C1 New York Harbor VA Med Ctr, Dept Pathol & Lab Med, Brooklyn, NY 11209 USA. Long Isl Univ, Dept Biol, Brooklyn, NY 11201 USA. Long Isl Univ, Dept Chem, Brooklyn, NY 11201 USA. QRNA Corp, New York, NY 10032 USA. NCI, Lab Metab, Bethesda, MD 20892 USA. SUNY, Downstate Med Ctr, Dept Pathol, Brooklyn, NY 11203 USA. RP Pincus, MR (reprint author), New York Harbor VA Med Ctr, Dept Pathol & Lab Med, Brooklyn, NY 11209 USA. EM matthew.pincus2@med.va.gov RI Friedman, Fred/D-4208-2016 FU NCI NIH HHS [CA 42500] NR 18 TC 5 Z9 5 U1 0 U2 0 PU KLUWER ACADEMIC/PLENUM PUBL PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1572-3887 J9 PROTEIN J JI Protein J. PD APR PY 2004 VL 23 IS 3 BP 235 EP 238 DI 10.1023/B:JOPC.0000026419.54902.bb PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 838YU UT WOS:000222751300007 PM 15200055 ER PT J AU Panchenko, AR Kondrashov, F Bryant, S AF Panchenko, AR Kondrashov, F Bryant, S TI Prediction of functional sites by analysis of sequence and structure conservation SO PROTEIN SCIENCE LA English DT Article DE protein domains; prediction of functional residues; evolutionary conservation ID ENZYME ACTIVE-SITES; IMPORTANT RESIDUES; PROTEIN FAMILIES; BINDING SURFACES; PSI-BLAST; ALIGNMENTS; DATABASE; INTERFACES; GENERATION; EVOLUTION AB We present a method for prediction of functional sites in a set of aligned protein sequences. The method selects sites which are both well conserved and clustered together in space, as inferred from the 3D structures of proteins included in the alignment. We tested the method using 86 alignments from the NCBI CDD database, where the sites of experimentally determined ligand and/or macromolecular interactions are annotated. In agreement with earlier investigations, we found that functional site predictions are most successful when overall background sequence conservation is low, such that sites under evolutionary constraint become apparent. In addition, we found that averaging of conservation values across spatially clustered sites improves predictions under certain conditions: that is, when overall conservation is relatively high and when the site in question involves a large macromolecular binding interface. Under these conditions it is better to look for clusters of conserved sites than to look for particular conserved sites. C1 Natl Lib Med, Natl Ctr Biotechnol Informat, Computat Biol Branch, NIH, Bethesda, MD 20894 USA. Univ Calif Davis, Sect Evolut & Ecol, Davis, CA 95616 USA. RP Panchenko, AR (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, Computat Biol Branch, NIH, Bldg 38A,Rm 8N805, Bethesda, MD 20894 USA. EM panch@ncbi.nlm.nih.gov NR 34 TC 86 Z9 86 U1 0 U2 1 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI WOODBURY PA 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2924 USA SN 0961-8368 J9 PROTEIN SCI JI Protein Sci. PD APR PY 2004 VL 13 IS 4 BP 884 EP 892 DI 10.1110/ps.03465504 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 807AI UT WOS:000220474000005 PM 15010543 ER PT J AU Keskin, O Tsai, CJ Wolfson, H Nussinov, R AF Keskin, O Tsai, CJ Wolfson, H Nussinov, R TI A new, structurally nonredundant, diverse data set of protein-protein interfaces and its implications SO PROTEIN SCIENCE LA English DT Article DE data set of interfaces; protein binding; protein interfaces; protein-protein association; motifs, protein-protein interactions ID HOT-SPOTS; RECOGNITION SITES; BINDING; SEQUENCE; COMPLEXES; FAMILIES; RESIDUES; SURFACE; LIGANDS; CONSERVATION AB Here. we present a diverse, structurally nonredundant data set of two-chain protein-protein interfaces derived from the PDB. Using a sequence order-independent structural comparison algorithm and hierarchical clustering, 3799 interface clusters are obtained. These yield 103 clusters with at least five nonhomologous members. We divide the clusters into three types. In Type I clusters, the global structures of the chains from which the interfaces are derived are also similar. This cluster type is expected because, in general, related proteins associate in similar ways. In Type II, the interfaces are similar; however, remark ably, the overall structures and functions of the chains are different. The functional spectrum is broad, from enzymes/inhibitors to immunoglobulins and toxins. The fact that structurally different monomers associate in similar ways. suggests "good" binding architectures. This observation extends a paradigm in protein science: It has been well known that proteins with similar structures may have different functions. Here, we show that it extends to inter-faces. In Type III clusters, only one side of the interface is similar across the cluster. This structurally nonredundant data set provides rich data for studies of protein-protein interactions and recognition, cellular networks and drug design. In particular, it may be useful in addressing the difficult question of what are the favorable ways for proteins to interact. C1 SAIC Frederick Inc, Lab Expt & Computat Biol, Basic Res Program, NCI, Ft Detrick, MD 21702 USA. Koc Univ, Ctr Computat Biol & Bioinformat, TR-34450 Istanbul, Turkey. Koc Univ, Coll Engn, TR-34450 Istanbul, Turkey. Tel Aviv Univ, Dept Human Genet & Mol Med, Sackler Sch Med, Sch Comp Sci, IL-69978 Tel Aviv, Israel. Tel Aviv Univ, Dept Human Genet & Mol Med, Sackler Sch Med, Sackler Inst Mol Med, IL-69978 Tel Aviv, Israel. RP Keskin, O (reprint author), SAIC Frederick Inc, Lab Expt & Computat Biol, Basic Res Program, NCI, Bldg 469,Room 151, Ft Detrick, MD 21702 USA. EM ruthn@ncifcrf.gov RI Wolfson, Haim/A-1837-2011 FU NCI NIH HHS [N01-CO-12400, N01CO12400] NR 54 TC 139 Z9 143 U1 4 U2 7 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI WOODBURY PA 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2924 USA SN 0961-8368 J9 PROTEIN SCI JI Protein Sci. PD APR PY 2004 VL 13 IS 4 BP 1043 EP 1055 DI 10.1110/ps.03484604 PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 807AI UT WOS:000220474000019 PM 15044734 ER PT J AU Vitorino, R Lobo, MJC Ferrer-Correira, AJ Dubin, JR Tomer, KB Domingues, PM Amado, FML AF Vitorino, R Lobo, MJC Ferrer-Correira, AJ Dubin, JR Tomer, KB Domingues, PM Amado, FML TI Identification of human whole saliva protein components using proteomics SO PROTEOMICS LA English DT Article DE matrix assisted laser desorption; ionization-time of flight; saliva; two-dimensional gel electrophoresis ID ASSISTED-LASER-DESORPTION/IONIZATION; TANDEM MASS-SPECTROMETRY; CARBONIC-ANHYDRASE; 2-DIMENSIONAL ELECTROPHORESIS; HUMAN NASAL; PURIFICATION; FLUID; VI; PROLACTIN; SA AB The determination of salivary biomarkers as a means of monitoring general health and for the early diagnosis of disease is of increasing interest in clinical research. Based on the linkage between salivary proteins and systemic diseases, the aim of this work was the identification of saliva proteins using proteomics. Salivary proteins were separated using two-dimensional (2-D) gel electrophoresis over a pH range between 3-10, digested, and then analyzed by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF)-TOF mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Proteins were identified using automated MS and MS/MS data acquisition. The resulting data were searched against a protein database using an internal Mascot search routine. Ninety spots give identifications with high statistical reliability. Of the identified proteins, 11 were separated and identified in saliva for the first time using proteomics tools. Moreover, three proteins that have not been previously identified in saliva, PLUNC, cystatin A, and cystatin B were identified. C1 Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal. NIEHS, Struct Biol Lab, Res Triangle Pk, NC 27709 USA. RP Amado, FML (reprint author), Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal. EM famado@dq.ua.pt RI Tomer, Kenneth/E-8018-2013; Domingues, Pedro/E-5202-2010; Vitorino, Rui/G-7356-2014; Amado, Francisco/M-5337-2015 OI Domingues, Pedro/0000-0002-8060-7675; Vitorino, Rui/0000-0003-3636-5805; CALHEIROS-LOBO, MARIA JOAO/0000-0003-1692-9108; Amado, Francisco/0000-0001-8256-1749 NR 31 TC 171 Z9 174 U1 5 U2 42 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1615-9853 J9 PROTEOMICS JI Proteomics PD APR PY 2004 VL 4 IS 4 BP 1109 EP 1115 DI 10.1002/pmic.200300638 PG 7 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 811HV UT WOS:000220763900021 PM 15048992 ER PT J AU Chertov, O Biragyn, A Kwak, LW Simpson, JT Boronina, T Hoang, VM Prieto, DA Conrads, TP Veenstra, TD Fisher, RJ AF Chertov, O Biragyn, A Kwak, LW Simpson, JT Boronina, T Hoang, VM Prieto, DA Conrads, TP Veenstra, TD Fisher, RJ TI Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry SO PROTEOMICS LA English DT Article DE apolipoprotein A-II; biomarkers; serum; surface-enhanced laser desorption; ionization; tumor ID APOLIPOPROTEIN A-II; SEQUENCE; CANCER AB A method to extract peptides and low molecular weight proteins from serum under denaturing conditions using acetonitrile containing 0.1 % trifluoroacetic acid has been developed. The extraction procedure precipitates large, abundant proteins to simplify subsequent mass spectral analysis. This sample preparation method provides an efficient way to extract serum peptides, enabling them to be compared and identified using different mass spectrometry approaches. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry analysis of mouse blood serum samples prepared by this method allowed detection of two markers which were significantly reduced in mice with B cell lymphoma tumor. One of these markers has been identified as apolipoprotein A-II. C1 SAIC Frederick, Prot Chem Lab, Frederick, MD USA. SAIC Frederick, Mass Spect Ctr, Frederick, MD USA. RP Chertov, O (reprint author), NCI Frederick, Canc Res Ctr, Expt Transplantat & Immunol Branch, POB B,Bldg 469,Rm 237, Ft Detrick, MD 21702 USA. EM oleg@ncifcrf.gov RI Fisher, Robert/B-1431-2009 FU NCI NIH HHS [N01-CO-12400] NR 15 TC 77 Z9 80 U1 3 U2 24 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY SN 1615-9853 J9 PROTEOMICS JI Proteomics PD APR PY 2004 VL 4 IS 4 BP 1195 EP 1203 DI 10.1002/pmic.200300677 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 811HV UT WOS:000220763900028 PM 15048999 ER PT J AU Orain-Pelissolo, S Grillon, C Perez-Diaz, F Jouvent, R AF Orain-Pelissolo, S Grillon, C Perez-Diaz, F Jouvent, R TI Lack of startle modulation by smoking cues in smokers SO PSYCHOPHARMACOLOGY LA English DT Article DE smoking cue; acoustic startle reflex; prepulse inhibition; affective modulation ID FEAR-POTENTIATED STARTLE; PREPULSE INHIBITION; ACOUSTIC STARTLE; NICOTINE DEPENDENCE; REFLEX MODULATION; BLINK REFLEX; HUMANS; ATTENTION; EMOTION; PICTURES AB Rationale. The startle reflex methodology has been used to study the effects of nicotine in humans and the motivational effects of smoking cues in smokers. However, no other studies investigate startle modulation by smoking cues in smokers compared to non-smokers. In the other studies, smoking deprivation was manipulated in smokers or smokers were not compared directly to non-smokers. Objective. The goal of this study was to examine the temporal course of information processing following the presentation of a smoking-related cue using the startle probe methodology in smokers compared to non-smokers. Methods. Thirty-four smokers were selected on the basis of nicotinic dependence according to the DSM-IV, and compared to 34 non-smokers. During testing, subjects viewed neutral pictures and smoking related pictures displayed on a computer screen. Acoustic startle stimuli were delivered at various times after picture onset (60, 120 or 5000 ms) to examine inhibition by lead stimulus and the affective modulation of startle. Results. The magnitude of startle reflex inhibition increased in smokers compared to non-smokers, at 60 and 120 ms. In all, there was no PicturexGroup interaction effect. Conclusion. We showed that smoking cues have no impact on the startle reflex of either group, even if, in line with previous results, prepulse inhibition was higher in smokers than non-smokers. These results suggest that smoking cues have no effect on the positive reinforcement of nicotine consumption, and that cognitive factors play a primary role in the development and maintenance of tobacco dependence. C1 Hop La Pitie Salpetriere, CNRS UMR 7593, F-75013 Paris, France. NIMH, MAP, NIH, Unit Affect Psychophysiol, Bethesda, MD 20892 USA. RP Orain-Pelissolo, S (reprint author), Hop La Pitie Salpetriere, CNRS UMR 7593, Pavillon Clerambault,47 Blvd Hop, F-75013 Paris, France. EM orain@ext.jussieu.fr NR 49 TC 24 Z9 24 U1 0 U2 1 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 2004 VL 173 IS 1-2 BP 160 EP 166 DI 10.1007/s00213-003-1715-4 PG 7 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 814NQ UT WOS:000220981800021 PM 14726999 ER PT J AU Justinova, Z Tanda, G Munzar, P Goldberg, SR AF Justinova, Z Tanda, G Munzar, P Goldberg, SR TI The opioid antagonist naltrexone reduces the reinforcing effects of Delta(9)-tetrahydrocannabinol (THC) in squirrel monkeys SO PSYCHOPHARMACOLOGY LA English DT Article DE Delta(9)-tetrahydrocannabinol (THC); cocaine; naltrexone; drug self-administration; squirrel monkeys; marijuana ID RHESUS-MONKEYS; CANNABINOID RECEPTORS; ALCOHOL DEPENDENCE; NUCLEUS-ACCUMBENS; AGONIST CP-55,940; DELTA-RECEPTOR; FIXED-RATIO; NALOXONE; COCAINE; HEROIN AB Rationale. Experimental evidence from animal studies suggests reciprocal functional interactions between endogenous brain cannabinoid and opioid systems. There is recent evidence for a role of the opioid system in the modulation of the reinforcing effects of synthetic cannabinoid CB1 receptor agonists in rodents. Since Delta(9)-tetrahydrocannabinol (THC), the natural psychoactive ingredient in marijuana, is actively and persistently self-administered by squirrel monkeys, this provides an opportunity to directly study involvement of opioid systems in the reinforcing effects of THC in non-human primates. Objectives. To study the effects of naltrexone, an opioid antagonist, on THC self-administration behavior in squirrel monkeys. Methods. Monkeys pressed a lever for intravenous injections of THC under a ten-response, fixed-ratio (FR) schedule with a 60-s time-out after each injection. Effects of pre-session treatment with naltrexone (0.03-0.3 mg/kg intramuscularly, 15 min before session) for 5 consecutive days on self-administration of different doses of THC (2-8 mug/kg per injection) were studied. Results. Self-administration responding for THC was significantly reduced by pretreatment with 0.1 mg/kg naltrexone for five consecutive daily sessions. Naltrexone pretreatment had no significant effect on cocaine self-administration responding under identical conditions. Conclusions. Self-administration behavior under a fixed-ratio schedule of intravenous THC injection was markedly reduced by daily pre-session treatment with naltrexone, but remained above saline self-administration levels. These findings demonstrate for the first time the modulation of the reinforcing effects of THC by an opioid antagonist in a non-human primate model of marijuana abuse. C1 NIDA, US Dep HHS,NIH, Intramural Res Program, Behav Neurosci Res Branch,Preclin Pharmacol Sect,, Baltimore, MD 21224 USA. NIDA, US Dept HHS,NIH, Intramural Res Program, Medicat Discovery Res Branch,Psychobiol Sect, Baltimore, MD 21224 USA. ALEXZA Mol Delivery Corp, Palo Alto, CA 94303 USA. RP Goldberg, SR (reprint author), NIDA, US Dep HHS,NIH, Intramural Res Program, Behav Neurosci Res Branch,Preclin Pharmacol Sect,, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. EM sgoldber@intra.nida.nih.gov RI Tanda, Gianluigi/B-3318-2009; Justinova, Zuzana/A-9109-2011 OI Tanda, Gianluigi/0000-0001-9526-9878; Justinova, Zuzana/0000-0001-5793-7484 NR 53 TC 65 Z9 70 U1 2 U2 4 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 2004 VL 173 IS 1-2 BP 186 EP 194 DI 10.1007/s00213-003-1693-6 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 814NQ UT WOS:000220981800024 PM 14668977 ER PT J AU Singh, J Zarate, CA Krystal, AD AF Singh, J Zarate, CA Krystal, AD TI Case report: successful riluzole augmentation therapy in treatment-resistant bipolar depression following the development of rash with lamotrigine SO PSYCHOPHARMACOLOGY LA English DT Letter ID DISORDERS C1 NIMH, Mol Pathophysiol Lab, Bethesda, MD 20892 USA. Duke Univ, Med Ctr, Durham, NC 27708 USA. RP Singh, J (reprint author), NIMH, Mol Pathophysiol Lab, Bldg 10,Room 3S242,9000 Rockville Pike, Bethesda, MD 20892 USA. EM singhj@intra.nimh.nih.gov RI Krystal, Andrew/J-7109-2013 OI Krystal, Andrew/0000-0002-6702-781X NR 11 TC 13 Z9 13 U1 0 U2 2 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 2004 VL 173 IS 1-2 BP 227 EP 228 DI 10.1007/s00213-003-1756-8 PG 2 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 814NQ UT WOS:000220981800031 PM 14722708 ER PT J AU Fiddick, L AF Fiddick, L TI Domains of deontic reasoning: Resolving the discrepancy between the cognitive and moral reasoning literatures SO QUARTERLY JOURNAL OF EXPERIMENTAL PSYCHOLOGY SECTION A-HUMAN EXPERIMENTAL PSYCHOLOGY LA English DT Article ID WASON SELECTION TASK; CHILDRENS CONCEPTIONS; SOCIAL-EXCHANGE; PARENTAL AUTHORITY; NATURAL-SELECTION; JUDGMENTS; RULES; TRANSGRESSIONS; ADOLESCENTS; PERSPECTIVE AB Deontic reasoning has been studied in two subfields of psychology: the cognitive and moral reasoning literatures. These literatures have drawn different conclusions about the nature of deontic reasoning. The consensus within the cognitive reasoning literature is that deontic reasoning is a unitary phenomenon, whereas the consensus within the moral reasoning literature is that there are different subdomains of deontic reasoning. We present evidence from a series of experiments employing the methods of both literatures suggesting that people make a systematic distinction between two types of deontic rule: social contracts and precautions. The results call into question the prevailing opinion in the cognitive reasoning literature and provide further support for both an evolutionary view of deontic reasoning and the more domain-specific perspective found in the moral reasoning literature. C1 Max Planck Inst Human Dev, Berlin, Germany. RP Fiddick, L (reprint author), NINDS, NIH, CNS, Bldg 10,Room 5C205, Bethesda, MD 20892 USA. EM fiddickL@ninds.nih.gov NR 65 TC 40 Z9 43 U1 1 U2 4 PU PSYCHOLOGY PRESS PI HOVE PA 27 CHURCH RD, HOVE BN3 2FA, EAST SUSSEX, ENGLAND SN 0272-4987 J9 Q J EXP PSYCHOL-A JI Q. J. Exp. Psychol. Sect A-Hum. Exp. Psychol. PD APR PY 2004 VL 57 IS 3 BP 447 EP 474 DI 10.1080/02724980343000332 PG 28 WC Psychology; Psychology, Experimental SC Psychology GA 804OQ UT WOS:000220308400004 PM 15204136 ER PT J AU Stezhko, VA Buglova, EE Danilova, LI Drozd, VM Krysenko, NA Lesnikova, NR Minenko, VF Ostapenko, VA Petrenko, SV Polyanskaya, ON Rzheutski, VA Tronko, MD Bobylyova, OO Bogdanova, TI Ephstein, OV Kairo, IA Kostin, OV Likhtarev, IA Markov, VV Oliynik, VA Shpak, VM Tereshchenko, VP Zamotayeva, GA Beebe, GW Bouville, AC Brill, AB Burch, JD Fink, DJ Greenebaum, E Howe, GR Luckyanov, NK Masnyk, IJ McConnell, RJ Robbins, J Thomas, TL Voilleque, PG Zablotska, LB AF Stezhko, VA Buglova, EE Danilova, LI Drozd, VM Krysenko, NA Lesnikova, NR Minenko, VF Ostapenko, VA Petrenko, SV Polyanskaya, ON Rzheutski, VA Tronko, MD Bobylyova, OO Bogdanova, TI Ephstein, OV Kairo, IA Kostin, OV Likhtarev, IA Markov, VV Oliynik, VA Shpak, VM Tereshchenko, VP Zamotayeva, GA Beebe, GW Bouville, AC Brill, AB Burch, JD Fink, DJ Greenebaum, E Howe, GR Luckyanov, NK Masnyk, IJ McConnell, RJ Robbins, J Thomas, TL Voilleque, PG Zablotska, LB CA Chornobyl Thyroid Diseases Study TI A cohort study of thyroid cancer and other thyroid diseases after the chornobyl accident: Objectives, design and methods SO RADIATION RESEARCH LA English DT Article ID RADIOACTIVE IODINE; I-131 THERAPY; FOLLOW-UP; HYPERTHYROIDISM; RADIATION; MORTALITY; RISK; BYELARUS; EXPOSURE; CHILDREN AB The thyroid gland in children is one of the organs that is most sensitive to external exposure to X and gamma rays. However, data on the risk of thyroid cancer in children after exposure to radioactive iodines are sparse. The Chornobyl accident in Ukraine in 1986 led to the exposure of large populations to radioactive iodines, particularly I-131. This paper describes an ongoing cohort study being conducted in Belarus and Ukraine that includes 25,161 subjects under the age of 18 years in 1986 who are being screened for thyroid diseases every 2 years. Individual thyroid doses are being estimated for all study subjects based on measurement of the radioactivity of the thyroid gland made in 1986 together with a radioecological model and interview data. Approximately 100 histologically confirmed thyroid cancers were detected as a consequence of the first round of screening. The data will enable fitting appropriate dose-response models, which are important in both radiation epidemiology and public health for prediction of risks from exposure to radioactive iodines from medical sources and any future nuclear accidents. Plans are to continue to follow-up the cohort for at least three screening cycles, which will lead to more precise estimates of risk. (C) 2004 by Radiation Research Society. C1 Columbia Univ, Mailman Sch Publ Hlth, Dept Epidemiol, New York, NY 10032 USA. Minist Hlth, Minsk, Byelarus. Res Clin Inst Radiat Med & Endocrinol, Minsk, Byelarus. Natl Acad Postgrad Med Training, Dept Endocrinol, Minsk, Byelarus. Hlth Care Adm Gomel Oblast Execut Comm, Gomel, Byelarus. Natl Dispensary Radiat Med, Minsk, Byelarus. Inst Endocrinol & Metab, Kiev, Ukraine. Ukrainian Minist Hlth, Kiev, Ukraine. Ukraine Acad Med Sci, Sci Ctr Radiat Med, Kiev, Ukraine. NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA. Vanderbilt Univ, Sch Med, Dept Radiat & Radiol Sci, Nashville, TN 37212 USA. Columbia Univ, Coll Phys & Surg, Dept Pathol, New York, NY 10027 USA. Columbia Univ, Coll Phys & Surg, Dept Med, Thyroid Clin, New York, NY 10027 USA. NIDDKD, Genet & Biochem Branch, NIH, Bethesda, MD 20892 USA. MJP Risk Assessment Inc, Denver, CO USA. RP Masnyk, IJ (reprint author), Columbia Univ, Mailman Sch Publ Hlth, Dept Epidemiol, 722 W 168th St,Suite 1104, New York, NY 10032 USA. EM masnyki@mail.nih.gov RI Brill, Aaron/H-3732-2014 OI Brill, Aaron/0000-0001-7538-086X NR 31 TC 62 Z9 62 U1 1 U2 6 PU RADIATION RESEARCH SOC PI OAK BROOK PA 820 JORIE BOULEVARD, OAK BROOK, IL 60523 USA SN 0033-7587 J9 RADIAT RES JI Radiat. Res. PD APR PY 2004 VL 161 IS 4 BP 481 EP 492 DI 10.1667/3148 PG 12 WC Biology; Biophysics; Radiology, Nuclear Medicine & Medical Imaging SC Life Sciences & Biomedicine - Other Topics; Biophysics; Radiology, Nuclear Medicine & Medical Imaging GA 807ZB UT WOS:000220538300015 PM 15038762 ER PT J AU Schellinger, RD Fiebach, JB AF Schellinger, RD Fiebach, JB TI Value of modern CT techniques in the diagnosis of acute stroke SO RADIOLOGE LA German DT Article DE stroke; thrombolysis; CT-angiography (CTA); CTA source image analysis (CTA-SI); perfusion CT (PCT) ID ACUTE ISCHEMIC-STROKE; THROMBOLYTIC THERAPY; INTRAARTERIAL THROMBOLYSIS; INTRAVENOUS THROMBOLYSIS; PLASMINOGEN-ACTIVATOR; COMPUTED-TOMOGRAPHY; CEREBRAL-ISCHEMIA; ANGIOGRAPHY; PERFUSION; INFARCTION AB Background. Thrombolysis is the treatment of choice for acute stroke within 3 h after symptom onset. Treatment beyond the 3 h time window has not been shown to be effective in any single trial, however, metaanalyses suggest a somewhat less but still significant effect within 3 to 6 h after stroke. It seems reasonable to apply improved selection criteria that allow to differentiate the patients with a relevant indication for thrombolytic therapy from those who have not. While stroke MRI seems to be the upcoming standard, due to its low availability the need for an improved CT-based patient selection is evident. Methods. The present literature on imaging in stroke has been thoroughly reviewed. The diagnostic strengths and weaknesses of conventional CT, CT angiography (CTA), CTA source image analysis (CTA-SI) and perfusion CT (PCT) for an acute diagnostic stroke workup are critically reviewed in this article. The authors present their view about a comprehensive diagnostic approach to acute stroke in accordance to stroke MRI findings,which allows to challenge the rigid therapeutic time window and improve patient management. Conclusion. Information about the presence or absence of ICH by non contrast CT and vessel occlusion by means of CTA is deemed obligatory before rt-PA is given in the 3-6 hour time window. Clear demarcation of an early hypodensity exceeding 1/3 of the MCA territory on NCCT or CTA-SI should preclude thrombolytic therapy. The irreversibly damaged infarct core and the ischemic but still viable thus salvageable tissue at risk of infarction as seen on CT/CTA/CTA-SI/PCT should be obtained before thrombolysis is initiated within 3-6 hours. Once these advanced techniques are used,the therapeutic time window can be extended with acceptable safety. However, comprehensive informed consent is mandatory, especially when thrombolytic therapy is considered beyond established time windows. C1 Univ Klinikum Heidelberg, Neurol Klin, Heidelberg, Germany. Univ Klin Heidelberg, Abt Neuroradiol, Heidelberg, Germany. NINDS, NIH, Bethesda, MD 20892 USA. RP Schellinger, RD (reprint author), Univ Heidelberg, Neurol Klin, Neuenheimer Feld 400, D-69120 Heidelberg, Germany. EM Peter_Schellinger@med.uni-heidelberg.de OI Fiebach, Jochen B./0000-0002-7936-6958 NR 33 TC 4 Z9 4 U1 0 U2 0 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-832X J9 RADIOLOGE JI Radiologe PD APR PY 2004 VL 44 IS 4 BP 380 EP 388 DI 10.1007/s00117-003-1003-7 PG 9 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 820YP UT WOS:000221426400010 ER PT J AU Hoodbhoy, T Dean, J AF Hoodbhoy, T Dean, J TI Insights into the molecular basis of sperm-egg recognition in mammals SO REPRODUCTION LA English DT Review ID ZONA-PELLUCIDA GLYCOPROTEINS; MOUSE EGGS; TRANSGENIC MICE; KNOCKOUT MICE; IN-VITRO; ZP3; FERTILIZATION; OOCYTES; RECEPTORS; PROTEINS AB The zona pellucida surrounding the egg and pre-implantation embryo is required for in vivo fertility and early development. Explanatory models of sperm-egg recognition need to take into account the ability of sperm to bind to ovulated eggs, but not to two-cell embryos. For the last two decades, investigators have sought to identify an individual protein or carbohydrate side chain as the 'sperm receptor'. However, recent genetic data in mice are more consistent with the three-dimensional structure of the zona pellucida, rather than a single protein (or carbohydrate), determining sperm binding. The mouse and human zonae pellucidae contain three glycoproteins (ZP1, ZP2, ZP3) and, following fertilization, ZP2 is proteolytically cleaved. The replacement of endogenous mouse proteins with human ZP2, ZP3 or both does not alter taxon specificity of sperm binding or prevent fertility. Surprisingly, human ZP2 is not cleaved following fertilization and intact ZP2 correlates with persistent sperm binding to two-cell embryos. Taken together, these data support a model in which the cleavage status of ZP2 modulates the three-dimensional structure of the zona pellucida and determines whether sperm bind (uncleaved) or do not (cleaved). C1 NIDDK, Cellular & Dev Biol Lab, Natl Inst Hlth, Bethesda, MD 20892 USA. RP Hoodbhoy, T (reprint author), NIDDK, Cellular & Dev Biol Lab, Natl Inst Hlth, Bldg 50,Room 3128,50 South Drive,MSC 8028, Bethesda, MD 20892 USA. EM tanyah@intra.niddk.nih.gov NR 49 TC 89 Z9 100 U1 0 U2 3 PU BIO SCIENTIFICA LTD PI BRISTOL PA EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT, ENGLAND SN 1470-1626 J9 REPRODUCTION JI Reproduction PD APR PY 2004 VL 127 IS 4 BP 417 EP 422 DI 10.1530/rep.1.00181 PG 6 WC Developmental Biology; Reproductive Biology SC Developmental Biology; Reproductive Biology GA 811WC UT WOS:000220801000003 PM 15047932 ER PT J AU Fields, RD AF Fields, RD TI The other half of the brain SO SCIENTIFIC AMERICAN LA English DT Article C1 NICHHD, Nervous Syst Dev & Plast Div, Rockville, MD 20852 USA. Univ Maryland, Neurosci & Cognit Sci Program, College Pk, MD 20742 USA. RP Fields, RD (reprint author), NICHHD, Nervous Syst Dev & Plast Div, Rockville, MD 20852 USA. NR 5 TC 29 Z9 31 U1 0 U2 6 PU SCI AMERICAN INC PI NEW YORK PA 415 MADISON AVE, NEW YORK, NY 10017 USA SN 0036-8733 J9 SCI AM JI Sci.Am. PD APR PY 2004 VL 290 IS 4 BP 54 EP 61 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 801HR UT WOS:000220087500025 PM 15045754 ER PT J AU Takahashi, Y AF Takahashi, Y TI Identification and function of ubiquitin-like protein SUMO E3 (PIAS family and RanBp2, Pc2) SO SEIKAGAKU LA Japanese DT Review ID E3 LIGASE; GENE ACTIVATION; YEAST SEPTINS/; IN-VITRO; TRANSCRIPTION; SUMOYLATION; CONJUGATION; INHIBITION; ENCODES; COMPLEX C1 NICHD, NIH, LGRD, Bethesda, MD 20892 USA. RP Takahashi, Y (reprint author), NICHD, NIH, LGRD, 18T Lib Dr,Room 106, Bethesda, MD 20892 USA. NR 28 TC 1 Z9 1 U1 0 U2 0 PU JAPANESE BIOCHEMICAL SOC PI TOKYO PA ISHIKAWA BLDG-3F, 25-16 HONGO-5-CHOME, BUNKYO-KU, TOKYO, 113, JAPAN SN 0037-1017 J9 SEIKAGAKU JI Seikagaku PD APR PY 2004 VL 76 IS 4 BP 381 EP 384 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 818JH UT WOS:000221240900009 PM 15162967 ER PT J AU Shevach, EM AF Shevach, EM TI Regulatory T cells - Introduction SO SEMINARS IN IMMUNOLOGY LA English DT Editorial Material ID TOLERANCE C1 Natl Inst Allergy & Infect Dis, Lab Immunol, Cellular Immunol Sect, Bethesda, MD 20892 USA. RP Shevach, EM (reprint author), Natl Inst Allergy & Infect Dis, Lab Immunol, Cellular Immunol Sect, NIH Bldg 10,Room 11N315,10 Ctr Dr, Bethesda, MD 20892 USA. EM eshevach@niaid.nih.gov NR 7 TC 13 Z9 13 U1 0 U2 0 PU ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1044-5323 J9 SEMIN IMMUNOL JI Semin. Immunol. PD APR PY 2004 VL 16 IS 2 BP 69 EP 71 DI 10.1016/j.smim.2003.12.001 PG 3 WC Immunology SC Immunology GA 802XG UT WOS:000220195600001 PM 15036229 ER PT J AU Piccirillo, CA Shevach, EM AF Piccirillo, CA Shevach, EM TI Naturally-occurring CD4(+)CD25(+) immunoregulatory T cells: central players in the arena of peripheral tolerance SO SEMINARS IN IMMUNOLOGY LA English DT Review DE regulatory T cells; CD4(+)CD25(+); tolerance; suppression; immunoregulation; autoimmune disease; inflammation ID IMMUNOLOGICAL SELF-TOLERANCE; ORGAN-SPECIFIC AUTOIMMUNITY; CUTTING EDGE; IN-VITRO; DISEASE; HOMEOSTASIS; ACTIVATION; TRANSPLANTATION; REQUIREMENTS; PERSISTENCE AB Self/non-self discrimination is a complex process that involves maintaining tolerance to autoantigens while preserving the potential to generate an effective humoral and cellular immune responses against invading pathogens. In the last decade, there has been a remarkable resurgence in research on suppressor or regulatory T cells. Few areas have captivated the attention of immunologists so vividly and entertained so many passionate debates. Indeed, CD4(+)CD25(+) Treg, which are at the very center of this fascination, have emerged as a dominant T cell population capable of mediating peripheral tolerance to autoantigens, but whose functions have now been extended to regulation of T cell responses directed to foreign antigens. However, a number of fundamental questions concerning the origin, phenotypic nature and mechanism of action of CD4(+)CD25(+) Treg cells remain elusive and misunderstood. We propose the existence of two general subsets of CD4(+)CD25(+) Treg cells, naturally-occurring and induced, that differ in their origin, developmental and activation requirements, and mechanism of action. (C) 2004 Elsevier Ltd. All rights reserved. C1 McGill Univ, Host Resistance Lab, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada. NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Piccirillo, CA (reprint author), McGill Univ, Host Resistance Lab, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada. EM ciro.piccirillo@mcgill.ca NR 53 TC 263 Z9 288 U1 2 U2 4 PU ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 1044-5323 J9 SEMIN IMMUNOL JI Semin. Immunol. PD APR PY 2004 VL 16 IS 2 BP 81 EP 88 DI 10.1016/j.smim.2003.12.003 PG 8 WC Immunology SC Immunology GA 802XG UT WOS:000220195600003 PM 15036231 ER PT J AU Fowler, JS Volkow, ND Wang, GJ Ding, YS AF Fowler, JS Volkow, ND Wang, GJ Ding, YS TI 2-deoxy-2-[F-18]fluoro-D-glucose and alternative radiotracers for positron emission tomography Imaging using the human brain as a model SO SEMINARS IN NUCLEAR MEDICINE LA English DT Review ID MONOAMINE-OXIDASE B; BETA-AMYLOID PLAQUES; ALZHEIMERS-DISEASE; NICOTINIC RECEPTORS; GLUCOSE-METABOLISM; METHAMPHETAMINE ABUSERS; PARKINSONS-DISEASE; DOPAMINE; PET; COCAINE AB 2-Deoxy-2-[F-18]fluoro-D-glucose ((18)FDG) is now routinely available in many hospitals and other institutions either via on-site production or from one of the dozens of regional radiopharmacies worldwide. Its reliable production has opened the possibility for use in both basic and clinical investigations and also in pairing it with other more biologically specific positron emission tomography tracers to provide an important functional perspective to the measurement. In this article, we highlight examples in which (18)FDG is paired with another carbon-11- or fluorine-18-labeled radiotracer in the same subject to correlate neurotransmitter-specific effects with regional metabolic effects using the human brain as a model. We describe studies that fall into three major areas: normal aging, neuropsychiatric disorders, and drug action. (C) 2004 Elsevier Inc. All rights reserved. C1 Brookhaven Natl Lab, Dept Chem, Upton, NY 11973 USA. NIDA, Bethesda, MD 20892 USA. RP Fowler, JS (reprint author), Brookhaven Natl Lab, Dept Chem, Upton, NY 11973 USA. FU NIBIB NIH HHS [EB 002630] NR 63 TC 6 Z9 7 U1 2 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0001-2998 J9 SEMIN NUCL MED JI Semin. Nucl. Med. PD APR PY 2004 VL 34 IS 2 BP 112 EP 121 DI 10.1053/j.semnuclmed.2004.01.002 PG 10 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 805MM UT WOS:000220370400004 PM 15031811 ER PT J AU Stone, RM Gilliland, DG Klion, AD AF Stone, RM Gilliland, DG Klion, AD TI Platelet-derived growth factor receptor inhibition to treat idiopathic hypereosinophilic syndrome SO SEMINARS IN ONCOLOGY LA English DT Review ID IMATINIB MESYLATE; TYROSINE KINASE; MYELOPROLIFERATIVE VARIANT; BCR-ABL; C-KIT; THERAPY; CELLS; TRANSPLANTATION; MYELOFIBROSIS; CYCLOSPORINE C1 Dana Farber Canc Inst, Adult Leukemia Program, Boston, MA 02115 USA. Brigham & Womens Hosp, Boston, MA 02115 USA. NIAID, NIH, Bethesda, MD 20892 USA. RP Stone, RM (reprint author), Dana Farber Canc Inst, Adult Leukemia Program, 44 Binney St,Dana 840, Boston, MA 02115 USA. OI Klion, Amy/0000-0002-4986-5326 NR 36 TC 8 Z9 8 U1 0 U2 2 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD APR PY 2004 VL 31 IS 2 SU 6 BP 12 EP 17 DI 10.1053/j.seminoncol.2004.030.35 PG 6 WC Oncology SC Oncology GA 824NQ UT WOS:000221693700003 PM 15175999 ER PT J AU Yancik, R Ries, LAG AF Yancik, R Ries, LAG TI Cancer in older persons: An international issue in an aging world SO SEMINARS IN ONCOLOGY LA English DT Review ID BURDEN C1 NIA, Geriatr & Clin Gerontol Program, NIH, Bethesda, MD 20892 USA. NCI, Canc Control Res Program, Div Canc Control & Populat Sci, NIH, Bethesda, MD USA. RP Yancik, R (reprint author), NIA, Geriatr & Clin Gerontol Program, NIH, Bethesda, MD 20892 USA. NR 22 TC 155 Z9 161 U1 0 U2 5 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD APR PY 2004 VL 31 IS 2 BP 128 EP 136 DI 10.1053/j.seminoncol.2003.12.024 PG 9 WC Oncology SC Oncology GA 813DA UT WOS:000220886600002 PM 15112144 ER PT J AU Westin, EH Longo, DL AF Westin, EH Longo, DL TI Lymphoma and myeloma in older patients SO SEMINARS IN ONCOLOGY LA English DT Review ID NON-HODGKINS-LYMPHOMA; B-CELL LYMPHOMA; HEALTH-ORGANIZATION CLASSIFICATION; ELDERLY-PATIENTS; MULTIPLE-MYELOMA; FOLLICULAR LYMPHOMA; AGGRESSIVE LYMPHOMA; RANDOMIZED-TRIAL; AGE; DISEASE C1 NIA, NIH, Hematol Oncol Sect, Clin Invest Lab, Baltimore, MD 21224 USA. RP Westin, EH (reprint author), NIA, NIH, Hematol Oncol Sect, Clin Invest Lab, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 51 TC 18 Z9 21 U1 1 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD APR PY 2004 VL 31 IS 2 BP 198 EP 205 DI 10.1053/j.seminoncol.2003.12.030 PG 8 WC Oncology SC Oncology GA 813DA UT WOS:000220886600008 PM 15112150 ER PT J AU Kreimer, AR Alberg, AJ Viscidi, R Gillison, ML AF Kreimer, AR Alberg, AJ Viscidi, R Gillison, ML TI Gender differences in sexual biomarkcers and behaviors associated with human papillomavirus-16,-18, and-33 seroprevalence SO SEXUALLY TRANSMITTED DISEASES LA English DT Article ID VIRUS-LIKE PARTICLES; SQUAMOUS-CELL CARCINOMA; HERPES-SIMPLEX-VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; CERVICAL INTRAEPITHELIAL NEOPLASIA; TYPE-16 CAPSID ANTIBODIES; ORAL HUMAN PAPILLOMAVIRUS; HPV INFECTION; UNITED-STATES; RISK FACTOR AB Background: The elevated risk for incident head and neck cancer among human papillomavirus (HPV)-16-seropositive individuals has substantiated a role for HPV in the etiology of head and neck cancers. The relationship between HPV seroreactivity and prevalent oral HPV infection in men and women without cancer has yet to be investigated. Goal: The goal of this study was to evaluate a possible association between oral HPV infection and HPV seroreactivity after adjustment for gender, sexual behaviors, and sexually transmitted disease. Study Design: A cross-sectional study of factors associated with HPV-16, -18, and -33 seroreactivity was performed in a population of 586 men and women with and without HIV infection. Antibodies in sera were measured by use of a virus-like protein (VLP)-based enzyme-linked immunosorbent assay. Exfoliated cells from the tonsillar and oral mucosa were analyzed for the presence of 38 mucosal HPV types by polymerase chain reaction. Results: Women had significantly greater seroreactivity for all HPV types investigated when compared with men (odds ratio, 4.3; 95% confidence interval, 3.0-6.0). Seroprevalence was greatest in men and women aged 35 to 45 years. Tonsillar HPV infection, oral sex with men, and HIV infection were independently associated with HPV seroreactivity in men after adjustment for age and number of sexual partners. In women, HSV-2 seropositivity and a history of sexually transmitted diseases were similarly important. Oral and tonsillar HPV infection were not associated with HPV seroreactivity in women. Conclusion: HPV seropositivity is associated with sexually transmitted diseases among women and possibly mucosal HPV exposures in men. Tonsillar HPV infection could impact seroprevalence, particularly in men. C1 Johns Hopkins, Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD USA. NCI, Canc Prevent Fellowship Program, Div Canc Prevent, NIH,DHHS, Bethesda, MD 20892 USA. Johns Hopkins Sch Med, Dept Pediat, Baltimore, MD USA. Johns Hopkins, Sidney Kimmel Comprehens Canc Ctr, Dept Oncol, Baltimore, MD 21231 USA. RP Gillison, ML (reprint author), Johns Hopkins, Sidney Kimmel Comprehens Canc Ctr, Dept Oncol, G91,Canc Res Bldg,1650 Orleans St, Baltimore, MD 21231 USA. EM gillima@jhmi.edu RI Kreimer, Aimee/H-1687-2015 FU NCI NIH HHS [K07 CA73790]; NIAID NIH HHS [AI-42058]; NIDCR NIH HHS [DE13121] NR 70 TC 44 Z9 44 U1 1 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0148-5717 J9 SEX TRANSM DIS JI Sex. Transm. Dis. PD APR PY 2004 VL 31 IS 4 BP 247 EP 256 DI 10.1097/01.OLQ.0000118425.49522.2C PG 10 WC Infectious Diseases SC Infectious Diseases GA 807ZF UT WOS:000220538700011 PM 15028941 ER PT J AU Alving, B AF Alving, B TI NIH asks participants in women's health initiative estrogen-alone study to stop study pills, begin follow-up phase SO SOUTHERN MEDICAL JOURNAL LA English DT Article C1 NHLBI, NIH, Bethesda, MD 20892 USA. RP Alving, B (reprint author), NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 4 Z9 4 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-4348 J9 SOUTH MED J JI South.Med.J. PD APR PY 2004 VL 97 IS 4 BP 425 EP 426 DI 10.1097/01.SMJ.0000125549.20351.BB PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 831DC UT WOS:000222172200028 PM 15108846 ER PT J AU Ihara, M Tomimoto, H Ishizu, K Mukai, T Yoshida, H Sawamoto, N Inoue, M Doi, T Hashikawa, K Konishi, J Shibasaki, H Fukuyama, H AF Ihara, M Tomimoto, H Ishizu, K Mukai, T Yoshida, H Sawamoto, N Inoue, M Doi, T Hashikawa, K Konishi, J Shibasaki, H Fukuyama, H TI Decrease in cortical benzodiazepine receptors in symptomatic patients with leukoaraiosis - A positron emission tomography study SO STROKE LA English DT Article DE leukoaraiosis; Binswanger's disease; receptors, benzodiazepine; tomography, emission computed ID WHITE-MATTER LESIONS; CEREBRAL BLOOD-FLOW; VASCULAR DEMENTIA; BINSWANGERS-DISEASE; OXYGEN-METABOLISM; BRAIN; HYPERINTENSITIES; INDIVIDUALS; FLUMAZENIL; IMPAIRMENT AB Background and Purpose -[C-11] flumazenil (FMZ), a ligand that selectively binds to the central benzodiazepine receptor in the neuronal membrane, is useful for evaluating neuronal viability in a positron emission tomography ( PET) scan. Using this ligand, we investigated whether there was a correlation between neuronal integrity in various brain structures and dementia in patients with leukoaraiosis. Methods - Twelve patients with extensive leukoaraiosis on magnetic resonance imaging were divided into groups of patients with or without dementia. Based on a 2-compartment, 2-parameter model that included metabolite-corrected arterial input and PET-measured cerebral radioactivity, the distribution volume of FMZ (FMZ-V-d) was calculated in various regions of interest by nonlinear curve fitting. Additionally, tracer kinetic analysis was applied for voxel-by-voxel quantification of FMZ-V-d, and data analysis was performed by statistical parametric mapping. Results - The presence of dementia was associated with a reduced FMZ-V-d in widespread areas of the cerebral cortex, including the bilateral frontopolar and frontal/insular areas, the left temporo-occipital border areas, and the left marginal cortical areas. Conclusions - Differences in neuronal integrity in the cerebral cortex might determine whether patients with leukoaraiosis become symptomatic or not. C1 Kyoto Univ, Horizontal Med Res Org, Grad Sch Med, Sakyo Ku, Kyoto, Japan. Kyoto Univ, Grad Sch Med, Dept Neurol, Sakyo Ku, Kyoto, Japan. Kyoto Univ, Grad Sch Med, Dept Diagnost Radiol & Nucl Med, Sakyo Ku, Kyoto, Japan. Kyoto Univ, Grad Sch Med, Human Brain Res Ctr, Sakyo Ku, Kyoto, Japan. NINDS, NIH, Bethesda, MD USA. RP Ihara, M (reprint author), Kyoto Univ, Horizontal Med Res Org, Grad Sch Med, Sakyo Ku, Kyoto, Japan. EM ihara@kuhp.kyoto-u.ac.jp OI Ihara, Masafumi/0000-0002-7102-4048 NR 35 TC 26 Z9 32 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0039-2499 J9 STROKE JI Stroke PD APR PY 2004 VL 35 IS 4 BP 942 EP 947 DI 10.1161/01.STR.0000122624.32167.e0 PG 6 WC Clinical Neurology; Peripheral Vascular Disease SC Neurosciences & Neurology; Cardiovascular System & Cardiology GA 806RG UT WOS:000220450400026 PM 15001785 ER PT J AU Horne, MK Inkellis, E AF Horne, MK Inkellis, E TI Retention of lepirudin at the tip of a silicone catheter: a better catheter flush solution? SO SUPPORTIVE CARE IN CANCER LA English DT Article DE catheter; flushes; lepirudin; heparin ID HEPARIN-INDUCED THROMBOCYTOPENIA; RANDOMIZED CONTROLLED-TRIALS; CENTRAL VENOUS CATHETERS; RECOMBINANT HIRUDIN; ACCESS; METAANALYSIS; MAINTENANCE; BENEFIT; PORTS; BLOOD AB Because central venous catheters often become blocked by clot at their tip despite heparin flushes, a more effective anticoagulant is needed. We hypothesize that lepirudin, a recently introduced protein anticoagulant, might be more effective than heparin because of its tendency to adsorb to silicone, a commonly used catheter material. We preliminarily tested this hypothesis in vitro by measuring residual lepirudin and heparin activity at the tip of a catheter that had been submerged in a flowing stream of water for various periods of time. We observed that lepirudin is less readily removed than heparin from the catheter by fluid washing over it. This "slow-release" property of lepirudin might provide prolonged protection against clot formation at the catheter tip. A clinical trial will be necessary, however, to determine whether this property translates into significant improvement in catheter function. C1 NIH, Dept Lab Med, Hematol Serv, Bethesda, MD 20892 USA. RP Horne, MK (reprint author), NIH, Dept Lab Med, Hematol Serv, Bldg 10,Rm 2C306, Bethesda, MD 20892 USA. EM mhorne@mail.cc.nih.gov NR 22 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER-VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0941-4355 J9 SUPPORT CARE CANCER JI Support. Care Cancer PD APR PY 2004 VL 12 IS 4 BP 278 EP 281 DI 10.1007/s00520-004-0592-7 PG 4 WC Oncology; Health Care Sciences & Services; Rehabilitation SC Oncology; Health Care Sciences & Services; Rehabilitation GA 814HX UT WOS:000220966900010 PM 14968353 ER PT J AU Patel, JG Bartoszyk, GD Edwards, E Ashby, CR AF Patel, JG Bartoszyk, GD Edwards, E Ashby, CR TI The highly selective 5-hydroxytryptamine (5-HT)(2A) receptor antagonist, EMD 281014, significantly increases swimming and decreases immobility in male congenital learned helpless rats in the forced swim test SO SYNAPSE LA English DT Article DE serotonin(2A) antagonist; EMD 281014; forced swim test ID SUICIDE VICTIMS; BINDING; BRAIN AB We examined the effect of the highly selective 5-hydroxytryptamine (5-HT)(2A) receptor antagonist 7-{4-[2-(4-fluoro-phenyl)-ethyl]-piperazine-1-carbonyl}-1H-indole-3-carbonitrile HCl (EMD 281014) in congenital learned helpless male rats in the forced swim test. The administration of EMD-281014 (0.3-30 mg/kg i.p.) to congenital learned helpless rats dose-dependently and significantly (at 10 and 30 mg/kg) decreased immobility and increased swimming compared to vehicle-treated animals. Thus, EMD 281014 produces effects in the forced swim test resembling those of antidepressants. (C) 2004 Wiley-Liss, Inc. C1 St Johns Univ, PHS Dept, Jamaica, NY 11439 USA. Preclin Res, Dept CNS Res, D-64271 Darmstadt, Germany. Merck KGaA, D-64271 Darmstadt, Germany. NINDS, Bethesda, MD 20892 USA. RP Ashby, CR (reprint author), St Johns Univ, PHS Dept, 8000 Utopia Pkwy, Jamaica, NY 11439 USA. EM Crashby@ix.neteom.com NR 10 TC 30 Z9 30 U1 1 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0887-4476 J9 SYNAPSE JI Synapse PD APR PY 2004 VL 52 IS 1 BP 73 EP 75 DI 10.1002/syn.10308 PG 3 WC Neurosciences SC Neurosciences & Neurology GA 778RP UT WOS:000189254800008 PM 14755634 ER PT J AU Cohen, SM Klaunig, J Meek, E Hill, RN Pastoor, T Lehman-McKeeman, L Bucher, J Longfellow, DG Seed, J Dellarco, V Fenner-Crisp, P Patton, D AF Cohen, SM Klaunig, J Meek, E Hill, RN Pastoor, T Lehman-McKeeman, L Bucher, J Longfellow, DG Seed, J Dellarco, V Fenner-Crisp, P Patton, D TI Evaluating the human relevance of chemically induced animal tumors SO TOXICOLOGICAL SCIENCES LA English DT Editorial Material DE carcinogenic mode of action; human relevance of animal tumors; risk assessment; PPAR alpha agonists ID CARCINOGENIC MODES; INFORMATION; FRAMEWORK AB Defining the mode(s) of action by which chemicals induce tumors in laboratory animals has become a key to judgments about the relevance of such tumor data for human risk assessment. Frameworks for analyzing mode of action information appear in recent U.S. EPA and IPCS publications relating to cancer risk assessment. This FORUM paper emphasizes that mode of action analytical frameworks depend on both qualitative and quantitative evaluations of relevant data and information: (1) presenting key events in the animal mode of action, (2) developing a "concordance" table for side-by-side comparison of key events as defined in animal studies with comparable information from human systems, and (3) using data and information from mode of action analyses, as well as information on relative sensitivity and exposure, to make weight-of-evidence judgments about the relevance of animal tumors for human cancer assessments. The paper features a systematic analysis for using mode of action information from animal and human studies, based in part on case examples involving environmental chemicals and pharmaceuticals. C1 Univ Nebraska, Med Ctr, Dept Pathol Microbiol, ILSI RSI Steering Comm, Omaha, NE 69198 USA. Indiana Univ, Sch Med, Indianapolis, IN 46202 USA. Hlth Canada, Ottawa, ON K1A 0L2, Canada. US EPA, Washington, DC 20460 USA. Syngenta Crop Protect, Greensboro, NC 27419 USA. Bristol Myers Squibb Co, Princeton, NJ 08543 USA. NIEHS, Res Triangle Pk, NC 27709 USA. NCI, NIH, Bethesda, MD 20892 USA. Int Life Sci Inst, Washington, DC 20005 USA. RP Cohen, SM (reprint author), Univ Nebraska, Med Ctr, Dept Pathol Microbiol, ILSI RSI Steering Comm, 983135 Nebraska Med Ctr, Omaha, NE 69198 USA. EM scohen@unmc.edu NR 6 TC 98 Z9 99 U1 0 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 J9 TOXICOL SCI JI Toxicol. Sci. PD APR PY 2004 VL 78 IS 2 BP 181 EP 186 DI 10.1093/toxsci/kfh073 PG 6 WC Toxicology SC Toxicology GA 810YP UT WOS:000220739900003 PM 14737005 ER PT J AU Nwosu, VC Kissling, GE Trempus, CS Honeycutt, H French, JE AF Nwosu, VC Kissling, GE Trempus, CS Honeycutt, H French, JE TI Exposure of Tg.AC transgenic mice to benzene suppresses hematopoietic progenitor cells and alters gene expression in critical signaling pathways SO TOXICOLOGY AND APPLIED PHARMACOLOGY LA English DT Article DE benzene; Tg.AC; mouse; hematopoietic progenitor cells; leukemia; cDNA array ID C-MYC; INDUCED APOPTOSIS; INHALED BENZENE; CYCLE ARREST; RAS; MOUSE; DIFFERENTIATION; LEUKEMOGENESIS; HEMATOTOXICITY; GROWTH AB The effects of acute benzene (BZ) exposure on hematopoietic progenitor cells (HPCs) derived from bone marrow cells were studied using homozygous male nu-Ha-ras Tg.AC mice at 8-10 weeks of age. The mice were given 0.02% BZ in their drinking water for 28 days with the dose rate estimated to be 34 mg benzene/kg BW/day. Analysis of cultured HPCs indicated that BZ suppressed the proliferation of the multilineage colony forming unit-granulocyte, erythrocyte, macrophage, megakaryocyte (CFU-GEMM); colony forming unit-granulocyte, macrophage (CFU-GM); and blast forming unit erythrocyte/colony forming unit erythrocyte (BFUE/CFUE). A gene expression profile was generated using nylon arrays spotted with 23 cDNAs involved in selected signal pathways involved in cell distress, inflammation, DNA damage, cell cycle arrest, and apoptosis. Of the 23 marker genes, 6 (bax, c-fos, E124, hsf1, ikBa, and p57) were significantly (Mann-Whitney U tests. P < 0.05) overexpressed in BZ-exposed mice. Two genes (c-myc and IL-2) approached significance (at P = 0.053). The pattern of gene expression was consistent with BZ toxicity and the suppression of HPCs. (C) 2004 Elsevier Inc. All rights reserved. C1 N Carolina Cent Univ, Dept Biol, Durham, NC 27707 USA. Natl Inst Environm Hlth Sci, Biostat Branch, Res Triangle Pk, NC 27709 USA. Natl Inst Environm Hlth Sci, Natl Ctr Toxicogenomics, Canc Biol Grp, Res Triangle Pk, NC 27709 USA. Natl Inst Environm Hlth Sci, Mol Toxicol Lab, Res Triangle Pk, NC 27709 USA. RP Nwosu, VC (reprint author), N Carolina Cent Univ, Dept Biol, 1801 Fayetteville St, Durham, NC 27707 USA. EM vcnwosu@wpo.nccu.edu FU PHSPO CDC HHS [SO608049] NR 49 TC 8 Z9 8 U1 0 U2 0 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0041-008X J9 TOXICOL APPL PHARM JI Toxicol. Appl. Pharmacol. PD APR 1 PY 2004 VL 196 IS 1 BP 37 EP 46 DI 10.1016/j.taap.2003.11.010 PG 10 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA 812DO UT WOS:000220820400005 PM 15050406 ER PT J AU Germolec, DR AF Germolec, DR TI Sensitivity and predictivity in immunotoxicity testing: immune endpoints and disease resistance SO TOXICOLOGY LETTERS LA English DT Article; Proceedings Paper CT 41st Congress of the European-Societies-of-Toxicology CY SEP 28-OCT 01, 2003 CL Florence, Italy SP European Soc Toxicol DE immunotoxicity; disease resistance; infection; neoplasia; rodents ID KILLER-CELL ACTIVITY; HOST-RESISTANCE; RISK-ASSESSMENT; IMMUNOLOGICAL PARAMETERS; FLOW-CYTOMETRY; B6C3F1 MICE; SUPPRESSION; TOXICOLOGY; VALIDATION; MODEL AB In spite of extensive laboratory data on the effects of chemicals and drugs on immunologic parameters in laboratory animals, and a well established correlation between suppression of immune function and increased incidence and/or severity of certain infectious and neoplastic diseases, interpreting data from experimental immunotoxicology studies for risk assessment purposes has proved challenging. This is particularly true when the immunological effects are minimal-to-moderate in nature, as might be expected from inadvertent chemical exposures. This review examines the methods used to evaluate immune responses in laboratory rodents and their utility to predict disease outcomes. The available data suggest that if a large enough population is exposed and that the challenge dose or virulence of pathogenic organisms or tumor cells is sufficient, small changes in immune surveillance could increase the background incidence and burden of disease in the human population. (C) 2004 Elsevier Ireland Ltd. All rights reserved. C1 NIEHS, Mol Toxicol Lab, Res Triangle Pk, NC 27709 USA. RP Germolec, DR (reprint author), NIEHS, Mol Toxicol Lab, POB 12233,111 Alexander Dr, Res Triangle Pk, NC 27709 USA. EM germolec@niehs.nih.gov NR 34 TC 36 Z9 41 U1 1 U2 4 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD APR 1 PY 2004 VL 149 IS 1-3 BP 109 EP 114 DI 10.1016/j.toxlet.2003.12.025 PG 6 WC Toxicology SC Toxicology GA 815TA UT WOS:000221063400015 PM 15093255 ER PT J AU Baccarelli, A Pesatori, AC Masten, SA Patterson, DG Needham, LL Mocarelli, P Caporaso, NE Consonni, D Grassman, JA Bertazzi, PA Landi, MT AF Baccarelli, A Pesatori, AC Masten, SA Patterson, DG Needham, LL Mocarelli, P Caporaso, NE Consonni, D Grassman, JA Bertazzi, PA Landi, MT TI Aryl-hydrocarbon receptor-dependent pathway and toxic effects of TCDD in humans: a population-based study in Seveso, Italy SO TOXICOLOGY LETTERS LA English DT Article; Proceedings Paper CT 41st Congress of the European-Societies-of-Toxicology CY SEP 28-OCT 01, 2003 CL Florence, Italy SP European Soc Toxicol DE dioxin; TCDD; molecular epidemiology; aryl-hydrocarbon receptor; population-based study ID DIOXIN EXPOSURE; ACCIDENT; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; ANIMALS AB Approximately 20 years after the Seveso, Italy accident, we conducted a population-based study to evaluate the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure upon immune and mechanistically based biomarkers of dioxin response in humans. TCDD toxic effects are known to be mediated by the aryl-hydrocarbon receptor (AhR). We randomly selected 62 study subjects from the highest exposed zones and 59 from the surrounding non-contaminated area. Current lipid-adjusted plasma TCDD concentrations in these subjects ranged from 3.5 to 90 ng/kg (or ppt) and were negatively associated with plasma IgG concentrations (r = -0.35; P = 0.0002). The expression of genes in the AhR-dependent pathway, including AhR, aryl-hydrocarbon receptor nuclear translocator (ARNT), CYP1A1, and CYP1B1 transcripts, and the CYP1A1-associated 7-ethoxyresorufin-O-deethylase (EROD) activity was measured in lymphocytes. AhR mRNA levels in uncultured lymphocytes were negatively associated with plasma TCDD (P = 0.03). When mitogen-induced lymphocytes were cultured with 10 nM TCDD, all AhR-dependent genes were induced 1.2- to 13-fold. In these cells, plasma TCDD was associated with decreased EROD activity. Markers within the AhR pathway were correlated with one another. Our findings suggest the presence of long-term effects in the subjects exposed to TCDD after the Seveso accident. (C) 2004 Elsevier Ireland Ltd. All rights reserved. C1 NCI, Genet Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, Bethesda, MD 20892 USA. Univ Milan, Ctr Res Occupat Clin & Environm Epidemiol, EPOCA, Dept Occupat & Environm Hlth, I-20122 Milan, Italy. Ist Clin Perfezionamento, Epidemiol Unit, Dept Occupat Hlth & Safety, Milan, Italy. NIEHS, Environm Toxicol Program, Res Triangle Pk, NC 27709 USA. Ctr Dis Control & Prevent, Natl Ctr Environm Hlth, Div Environm Hlth Lab Sci, Atlanta, GA USA. Univ Milan, Hosp Desio, Dept Lab Med, I-20122 Milan, Italy. CUNY Brooklyn Coll, Brooklyn, NY 11210 USA. RP Baccarelli, A (reprint author), NCI, Genet Epidemiol Branch, Div Canc Epidemiol & Genet, NIH,DHHS, 6120 Execut Blvd,EPS 7110, Bethesda, MD 20892 USA. EM baccarea@mail.nih.gov RI Needham, Larry/E-4930-2011; masten, scott/R-1403-2016; bertazzi, pietro alberto/D-5039-2017; OI masten, scott/0000-0002-7847-181X; bertazzi, pietro alberto/0000-0003-3475-2449; Baccarelli, Andrea/0000-0002-3436-0640; pesatori, angela/0000-0002-0261-3252 NR 15 TC 38 Z9 42 U1 4 U2 8 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0378-4274 J9 TOXICOL LETT JI Toxicol. Lett. PD APR 1 PY 2004 VL 149 IS 1-3 BP 287 EP 293 DI 10.1016/j.toxlet.2003.12.062 PG 7 WC Toxicology SC Toxicology GA 815TA UT WOS:000221063400035 PM 15093275 ER PT J AU Read, EJ Sullivan, MT AF Read, EJ Sullivan, MT TI Cellular therapy services provided by blood centers and hospitals in the United States, 1999: an analysis from the Nationwide Blood Collection and Utilization Survey SO TRANSFUSION LA English DT Article ID UMBILICAL-CORD BLOOD; TRANSPLANTATION; IMMUNOTHERAPY; MARROW AB BACKGROUND: The 2000 Nationwide Blood Collection and Utilization Survey was designed to assess cellular therapy product services in US blood centers and hospitals. STUDY DESIGN AND METHODS: Questionnaires were returned by 2,040 institutions. Data were analyzed for 30 quantitative variables related to cellular therapy product activities. RESULTS: 269 institutions, including 231 (12.2%) of the hospitals, 37 (25.9%) of the blood centers, and one cryobank, perforrmed HPC services. Collected PBSC (20,517) and cord blood products (12,628) far exceeded bone marrow (1,572), lymphocytes (578), and cultured cells (344). PBPC collections dropped 36.5 percent since the 1997 survey. Cord blood accounted for 35.4 percent of collections and 39.5 percent of products processed, but only 1.9 percent of infusions. CONCLUSIONS: Most cellular therapy services in hospitals and blood centers were HPC-related. The dramatic drop in PBPC collections since 1997 reflects the decline in autologous PBPC transplantation for breast cancer. Cord blood's high collection-to-infusion ratio demonstrates a substantial resource expenditure for banking a product for future clinical needs. Lymphocytes and cultured cell products contributed minimally to activities in this survey, but will likely increase in the future. Data from additional academic and commercial manufacturers of cellular therapy products should be. included in future surveys. C1 Warren G Magnuson Clin Ctr, Dept Transfus Med, NIH, Bethesda, MD 20892 USA. Natl Blood Data Resource Ctr, Bethesda, MD USA. RP Read, EJ (reprint author), Warren G Magnuson Clin Ctr, Dept Transfus Med, NIH, Bldg 10,Room 1C711, Bethesda, MD 20892 USA. EM eread@cc.nih.gov NR 15 TC 5 Z9 6 U1 0 U2 0 PU BLACKWELL PUBLISHING INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0041-1132 J9 TRANSFUSION JI Transfusion PD APR PY 2004 VL 44 IS 4 BP 539 EP 546 DI 10.1111/j.1537-2995.2003.03286.x PG 8 WC Hematology SC Hematology GA 807UL UT WOS:000220526300012 PM 15043570 ER PT J AU Carrington, M Cullen, M AF Carrington, M Cullen, M TI Justified chauvinism: advances in defining meiotic recombination through sperm typing SO TRENDS IN GENETICS LA English DT Review ID MAJOR HISTOCOMPATIBILITY COMPLEX; DOUBLE-STRAND-BREAK; CLASS-II REGION; HIGH-RESOLUTION ANALYSIS; MARIE-TOOTH-DISEASE; SINGLE HUMAN-SPERM; LINKAGE DISEQUILIBRIUM; HUMAN GENOME; HOT-SPOT; GENE CONVERSION AB Sperm typing offers an efficient means of studying the quantitative and qualitative aspects of meiotic recombination, that are virtually unapproachable by pedigree analysis. Since the initial development of the technique >10 years ago, several salient findings based on empirically derived recombination data have been described. The precise rates and distributions of recombination have been reported for specific regions of the genome, serving as the prototype for high-resolution genome-wide recombination patterns. Identification and characterization of molecular genetic events, such as unequal crossing over, gene conversion and crossover asymmetry, are under close inspection for the first time as a result of this technology. The influence of these phenomena on the evolution of the genome is of primary interest from a scientific and medical perspective. In this article, we review the novel discoveries in mammalian meiotic recombination that have been revealed through sperm typing. C1 NCI, Lab Genome Divers, SAIC Frederick, Frederick, MD 21702 USA. NCI, Tumor Angiogenesis Sect, Frederick, MD 21702 USA. RP Carrington, M (reprint author), NCI, Lab Genome Divers, SAIC Frederick, POB B,Bldg 560-21-89, Frederick, MD 21702 USA. EM carringt@ncifcrf.gov FU NCI NIH HHS [N01-CO-12400] NR 100 TC 26 Z9 28 U1 0 U2 3 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0168-9525 J9 TRENDS GENET JI Trends Genet. PD APR PY 2004 VL 20 IS 4 BP 196 EP 205 DI 10.1016/j.tig.2004.02.006 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA 812SJ UT WOS:000220858900006 PM 15041174 ER PT J AU Notkins, AL AF Notkins, AL TI Polyreactivity of antibody molecules SO TRENDS IN IMMUNOLOGY LA English DT Editorial Material ID B-CELL REPERTOIRE; MONOREACTIVE HIGH-AFFINITY; HIV-1 MONOCLONAL-ANTIBODY; CD5+ LYMPHOCYTE-B; ANTIGEN-BINDING; AUTOANTIBODY PRODUCTION; HUMAN-IMMUNOGLOBULIN; POSITIVE SELECTION; NATURAL ANTIBODIES; RHEUMATOID FACTORS AB The 'lock and key' hypothesis of antigen-antibody interaction has long dominated immunological thinking. However, studies demonstrating the existence of a large number of monoclonal antibodies that can bind to a variety of totally unrelated self and foreign antigens (i.e. polyreactive antibodies) has modified this view. We argue that the best explanation for polyreactivity is. that the antigen-binding 'pocket' of many antibody molecules is more flexible than previously thought and can change conformation to accommodate different antigens. In terms of biological function, we discuss the evidence for and against a role for polyreactive antibodies in immunological defense. We speculate that, paradoxically, because B cells that make polyreactive antibodies are capable of binding to many different endogenous host antigens, they might be involved in the induction and/or maintenance of immunological tolerance. C1 Natl Inst Dent & Craniofacial Res, Oral Infect & Immun Branch, Expt Med Sect, NIH, Bethesda, MD 20892 USA. NIH, Bethesda, MD 20892 USA. RP Notkins, AL (reprint author), Natl Inst Dent & Craniofacial Res, Oral Infect & Immun Branch, Expt Med Sect, NIH, Bethesda, MD 20892 USA. EM anotkins@mail.nih.gov NR 51 TC 155 Z9 160 U1 0 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1471-4906 J9 TRENDS IMMUNOL JI Trends Immunol. PD APR PY 2004 VL 25 IS 4 BP 174 EP 179 DI 10.1016/j.it.2004.02.004 PG 6 WC Immunology SC Immunology GA 813GK UT WOS:000220895400004 PM 15039043 ER PT J AU Freed, EO AF Freed, EO TI HIV-1 and the host cell: an intimate association SO TRENDS IN MICROBIOLOGY LA English DT Review ID IMMUNODEFICIENCY-VIRUS TYPE-1; TO-AUTOINTEGRATION FACTOR; FRIEND LEUKEMIA VIRUS; PREINTEGRATION COMPLEXES; CYCLOPHILIN-A; MULTIVESICULAR-BODY; RETROVIRUS RESTRICTION; MEMBRANE CHOLESTEROL; CHEMOKINE RECEPTORS; CDNA INTEGRATION AB As obligate parasites, viruses must rely heavily on host cell machinery to replicate and spread. Because of their relatively limited coding capacity, viruses with small genomes are particularly dependent on cellular factors. In the case of retroviruses, interactions with host cell machinery are evident at virtually every step in the replication cycle, from binding and entry to particle release. Recent studies on human immunodeficiency virus type 1 (HIV-1) have illustrated the extent to which retroviruses have evolved to usurp host cell processes and counter intracellular antiviral defenses. This review highlights the relationship between HIV-1 and host factors, and focuses on new developments in our understanding of how HIV-1 has evolved to use cellular machinery and avoid antiviral restriction. C1 NCI, Frederick Canc Res & Dev Ctr, HIV Drug Resistance Program, Virus Cell Interact Sect, Frederick, MD 21702 USA. RP Freed, EO (reprint author), NCI, Frederick Canc Res & Dev Ctr, HIV Drug Resistance Program, Virus Cell Interact Sect, Bldg 535 Rm,124 Sultan St, Frederick, MD 21702 USA. EM efreed@mail.nih.gov NR 70 TC 63 Z9 64 U1 0 U2 6 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0966-842X J9 TRENDS MICROBIOL JI Trends Microbiol. PD APR PY 2004 VL 12 IS 4 BP 170 EP 177 DI 10.1016/j.tim.2004.02.001 PG 8 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 815OM UT WOS:000221051600007 PM 15051067 ER PT J AU Hofseth, LJ Hussain, SP Harris, CC AF Hofseth, LJ Hussain, SP Harris, CC TI p53: 25 years after its discovery SO TRENDS IN PHARMACOLOGICAL SCIENCES LA English DT Article ID TUMOR-SUPPRESSOR GENE; BASE EXCISION-REPAIR; DNA-DAMAGE; SV40-TRANSFORMED CELLS; BREAST-CANCER; WILD-TYPE; MUTATIONS; ACTIVATION; APOPTOSIS; ANTIGEN AB Since its discovery 25 years ago, the p53 protein has emerged as a key tumor suppressor protein at the crossroads of cellular stress response pathways. Through these pathways, which can lead to cell-cycle arrest, DNA repair, cellular senescence, differentiation and apoptosis, p53 facilitates the repair and survival of damaged cells or eliminates severely damaged cells from the replicative pool to protect the organism. Because of these dynamic and multiple functions of p53, which are largely lost following mutations in the gene encoding p53, this molecule continues to be studied intensively in biomedical research, including the fields of toxicology and pharmacology. In this article, we briefly review the first 25 years of research on p53. C1 NCI, Canc Res Ctr, Human Carcinogenesis Lab, Bethesda, MD 20892 USA. RP Harris, CC (reprint author), Univ S Carolina, Coll Pharm, Columbia, SC 29208 USA. EM Curtis_Harris@nih.gov NR 42 TC 276 Z9 302 U1 1 U2 12 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0165-6147 J9 TRENDS PHARMACOL SCI JI Trends Pharmacol. Sci. PD APR PY 2004 VL 25 IS 4 BP 177 EP 181 DI 10.1016/j.tips.2004.02.009 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 815OL UT WOS:000221051500006 PM 15116721 ER PT J AU Zhu, G Evans, DM Duffy, DL Montgomery, GW Medland, SE Gillespie, NA Ewen, KR Jewell, M Liew, YW Hayward, NK Sturm, RA Trent, JM Martin, NG AF Zhu, G Evans, DM Duffy, DL Montgomery, GW Medland, SE Gillespie, NA Ewen, KR Jewell, M Liew, YW Hayward, NK Sturm, RA Trent, JM Martin, NG TI A genome scan for eye color in 502 twin families: Most variation is due to a QTL on chromosome 15q SO TWIN RESEARCH LA English DT Article ID TRAIT LINKAGE ANALYSIS; OCULOCUTANEOUS ALBINISM TYPE-2; VARIANCE-COMPONENTS; P-GENE; MICROSCOPIC FINDINGS; QUANTITATIVE TRAITS; GENOTYPING ERRORS; SKIN PIGMENTATION; PAIR LINKAGE; IRIS COLOR AB We have rated eye color on a 3-point scale (1=blue/grey, 2=hazel/green, 3=brown) in 502 twin families and carried out a 5-10 cM genome scan (400-757 markers). We analyzed eye color as a threshold trait and performed multipoint sib pair linkage analysis using variance components analysis in Mx. A lod of 19.2 was found at the marker D15S1002, less than 1 cM from OCA2, which has been previously implicated in eye color variation. We estimate that 74% of variance in eye color liability is due to this QTL and a further 18% due to polygenic effects. However, a large shoulder on this peak suggests that other loci affecting eye color may be telomeric of OCA2 and inflating the QTL estimate. No other peaks reached genome-wide significance, although lods >2 were seen on 5p and 14q and lods >1 were additionally seen on chromosomes 2, 3, 6, 7, 8, 9, 17 and 18. Most of these secondary peaks were reduced or eliminated when we repeated the scan as a two locus analysis with the 15q linkage included, although this does not necessarily exclude them as false positives. We also estimated the interaction between the 15q QTL and the other marker locus but there was only minor evidence for additive x additive epistasis. Elaborating the analysis to the full two-locus model including non-additive main effects and interactions did not strengthen the evidence for epistasis. We conclude that most variation in eye color in Europeans is due to polymorphism in OCA2 but that there may be modifiers at several other loci. C1 Queensland Inst Med Res, Brisbane, Qld 4029, Australia. Australian Genome Res Facil, Melbourne, Vic, Australia. Ctr Inherited Dis Res, Baltimore, MD USA. Australian Red Cross Blood Serv, Brisbane, Qld, Australia. Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia. Translat Genom Res Inst, Phoenix, AZ USA. Natl Canc Inst, Bethesda, MD USA. RP Martin, NG (reprint author), Queensland Inst Med Res, Brisbane, Qld 4029, Australia. EM nickM@qimr.edu.au RI Sturm, Richard/C-9943-2009; Gillespie, Nathan/D-6029-2013; Evans, David/H-6325-2013; Medland, Sarah/C-7630-2013; hayward, nicholas/C-1367-2015; Montgomery, Grant/B-7148-2008; Duffy, David/B-7392-2013; OI Sturm, Richard/0000-0003-1301-0294; Medland, Sarah/0000-0003-1382-380X; hayward, nicholas/0000-0003-4760-1033; Montgomery, Grant/0000-0002-4140-8139; Duffy, David/0000-0001-7227-632X; Evans, David/0000-0003-0663-4621; Martin, Nicholas/0000-0003-4069-8020 FU NCI NIH HHS [CA88363]; NHGRI NIH HHS [N01-HG-65403] NR 69 TC 84 Z9 86 U1 0 U2 2 PU AUSTRALIAN ACAD PRESS PI BOWEN HILLS PA 32 JEAYS ST, BOWEN HILLS, QLD 4006, AUSTRALIA SN 1369-0523 J9 TWIN RES JI Twin Res. PD APR PY 2004 VL 7 IS 2 BP 197 EP 210 DI 10.1375/136905204323016186 PG 14 WC Genetics & Heredity; Obstetrics & Gynecology; Reproductive Biology SC Genetics & Heredity; Obstetrics & Gynecology; Reproductive Biology GA 817UU UT WOS:000221203200012 PM 15169604 ER PT J AU Grubb, RL Collyer, WC Kibel, AS AF Grubb, RL Collyer, WC Kibel, AS TI Transitional cell carcinoma of the renal pelvis associated with hypercalcemia in a patient with autosomal dominant polycystic kidney disease SO UROLOGY LA English DT Article ID HYPER-CALCEMIA AB We present a case of transitional cell carcinoma (TCC) of the renal pelvis in a patient with autosomal dominant polycystic kidney disease (ADPKD) who presented with hypercalcemia. Hypercalcemia is rare in TCC and has not been independently associated with ADPKD. Preoperative magnetic resonance imaging findings suggested a diagnosis of TCC. The patient underwent left nephroureterectomy, which confirmed the diagnosis, and received adjuvant chemotherapy. The hypercalcemia had not recurred, and she was without evidence of disease recurrence at 36 months. This case is unique in that we did not find any previously reported cases of TCC associated with ADPKD and hypercalcemia. (C) 2004 Elsevier Inc. C1 Washington Univ, Sch Med, Div Urol, St Louis, MO USA. RP Grubb, RL (reprint author), Ctr Canc Res, Urol Oncol Branch, NCI, Bldg 10,Room 2B47,MSC 1501,9000 Rockville Pike, Bethesda, MD 20892 USA. NR 15 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0090-4295 J9 UROLOGY JI Urology PD APR PY 2004 VL 63 IS 4 DI 10.1016/j.urology.2003.12.005 PG 3 WC Urology & Nephrology SC Urology & Nephrology GA 811RW UT WOS:000220790000042 ER PT J AU Taraporewala, ZF Patton, JT AF Taraporewala, ZF Patton, JT TI Nonstructural proteins involved in genome packaging and replication of rotaviruses and other members of the Reoviridae SO VIRUS RESEARCH LA English DT Article DE icosahedrons; replication intermediates; Cryoelectron ID DOUBLE-STRANDED-RNA; HELIX-DESTABILIZING PROPERTIES; TEMPERATURE-SENSITIVE MUTANTS; CORE-SHELL PROTEIN; BLUETONGUE VIRUS; SIGMA-NS; BINDING PROTEIN; IN-VIVO; PHOSPHOHYDROLASE ACTIVITY; POLYMERASE-ACTIVITY AB Rotaviruses, members of family Reoviridae, are a major cause of acute gastroenteritis of infants and young children. The rotavirus genome consists of 11 segments of double-stranded (ds)RNA and the virion is an icosahedron composed of multiple layers of protein. The virion core is formed by a layer of VP2 and contains multiple copies of the RNA-dependent RNA polymerase VP1 and the mRNA-capping enzyme VP3. Double-layered particles (DLPs), representing cores surrounded by a layer of VP6, direct the synthesis of viral mRNAs. Rotavirus core- and DLP-like replication intermediates (RIs) catalyze the synthesis of dsRNA from viral template mRNAs coincidentally with the packaging of the mRNAs into the pre-capsid structures of RIs. In addition to structural proteins, the nonstructural proteins NSP2 and NSP5 are components of RIs with replicase activity. NSP2 self assembles into octameric structures that have affinity for ssRNA and NTPase and helix-destabilizing activites. Its interaction with nucleotides induces a conformational shift in the octamer to a more condensed form. Phosphate residues generated by the NTPase activity are believed to be transferred from NSP2 to NSP5, leading to the hyperphosphorylation of the latter protein. It is suspected that the transfer of the phosphate group to NSP5 allows NSP2 to return to its noncondensed state and, thus, to accept another NTP molecule. The NSP5-mediated cycling of NSP2 from condensed to noncondensed combined with its RNA binding and helix-destabilizing activities are consistent with NSP2 functioning as a molecular motor to facilitate the packaging of template mRNAs into the pre-capsid structures of RIs. Similarities with the bluetongue virus protein NS2 and the reovirus proteins sigmaNS and mu.2 suggest that they may be functional homologs of rotavirus NSP2 and NSP5. (C) 2003 Elsevier B.V. All rights reserved. C1 NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. RP Patton, JT (reprint author), NIAID, Infect Dis Lab, NIH, 50 South Dr MSC 8026,Room 6314, Bethesda, MD 20892 USA. EM jpatton@niaid.nih.gov RI Patton, John/P-1390-2014 NR 78 TC 44 Z9 47 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD APR PY 2004 VL 101 IS 1 BP 57 EP 66 DI 10.1016/j.virusres.2003.12.006 PG 10 WC Virology SC Virology GA 804VL UT WOS:000220326100006 PM 15010217 ER PT J AU Hunyady, L Gaborik, Z Shah, BH Jagadeesh, G Clark, AJL Catt, KJ AF Hunyady, L Gaborik, Z Shah, BH Jagadeesh, G Clark, AJL Catt, KJ TI Structural determinants of agonist-induced signaling and regulation of the angiotensin AT(1) receptor SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article; Proceedings Paper CT International Symposium on Aldosterone CY APR 28-30, 2003 CL London, ENGLAND SP Wellcome Trust, Pharmacia Inc, NIH, Astra Zeneca DE angiotensin II; Ca2+signaling; inositol phosphate responses; receptor internalization; site-directed mutagenesis ID II TYPE-1 RECEPTOR; PROTEIN-COUPLED RECEPTORS; 3RD INTRACELLULAR LOOP; CARBOXYL-TERMINAL TAIL; GROWTH-FACTOR RECEPTOR; INDUCED INTERNALIZATION; CYTOPLASMIC LOOP; RAT AT(1A); INDUCED PHOSPHORYLATION; TRANSMEMBRANE HELIX AB Angiotensin II (Ang II) regulates aldosterone secretion by stimulating inositol phosphate production and Ca2+ signaling in adrenal glomerulosa cells via the G(q)-coupled AT(1) receptor, which is rapidly internalized upon agonist binding. AngII also binds to the heptahelical AT(2) receptor, which neither activates inositol phosphate signaling nor undergoes receptor internalization. The differential behaviors of the AT, and AT2 receptors were analyzed in chimeric angiotensin receptors created by swapping the second (IL2), the third (IL3) intracellular loops and/or the cytoplasmic tail (CT) between these receptors. When transiently expressed in COS-7 cells, the chimeric receptors showed only minor alterations in their ligand binding properties. Measurements of the internalization kinetics and inositol phosphate responses of chimeric AT(1A) receptors indicated that the CT is required for normal receptor internalization, and IL2 is a determinant of G protein activation. In addition, the amino-terminal portion of IL3 is required for both receptor functions. However, only substitution of IL2 impaired Ang II-induced ERK activation, suggesting that alternative mechanisms are responsible for ERK activation in signaling-deficient mutant AT, receptors. Substitution of 1L2, 1L3, or CT of the AT(1A) receptor into the AT, receptor sequence did not endow the latter with the ability to internalize or to mediate inositol phosphate signaling responses. These data suggest that the lack of receptor internalization and inositol phosphate signal generation by the AT, receptor is a consequence of its different activation mechanism, rather than the inability of its cytoplasmic domains to couple to intracellular effectors. (C) 2003 Elsevier Ireland Ltd. All rights reserved. C1 Semmelweis Univ, Fac Med, Dept Physiol, H-1088 Budapest, Hungary. NICHHD, Endocrinol & Reprod Res Branch, NIH, Bethesda, MD 20892 USA. US FDA, Div Cardio Renal Drug Prod, Ctr Drug Evaluat & Res, Rockville, MD 20857 USA. Barts & London Queen Marys Sch Med & Dent, Dept Endocrinol, London EC1A 7BE, England. RP Hunyady, L (reprint author), Semmelweis Univ, Fac Med, Dept Physiol, H-1088 Budapest, Hungary. EM hunyady@puskin.sote.hu RI Jagadeesh, Gowraganahalli/G-6408-2010 NR 71 TC 9 Z9 10 U1 0 U2 1 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD MAR 31 PY 2004 VL 217 IS 1-2 BP 89 EP 100 DI 10.1016/j.mce.2003.10.014 PG 12 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 823TG UT WOS:000221635500013 PM 15134806 ER PT J AU Gorelik, J Zhang, YJ Shevchuk, AI Frolenkov, GI Sanchez, D Lab, MJ Vodyanoy, I Edwards, CRW Klenerman, D Korchev, YE AF Gorelik, J Zhang, YJ Shevchuk, AI Frolenkov, GI Sanchez, D Lab, MJ Vodyanoy, I Edwards, CRW Klenerman, D Korchev, YE TI The use of scanning ion conductance microscopy to image A6 cells SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article; Proceedings Paper CT International Symposium on Aldosterone CY APR 28-30, 2003 CL London, ENGLAND SP Wellcome Trust, Pharmacia Inc, NIH, Astra Zeneca DE scanning microscopy; A6 cells; aldosterone ID ATOMIC-FORCE MICROSCOPY; LIVING CELLS; SODIUM-CHANNELS; SURFACE; LOCALIZATION; ALDOSTERONE; DYNAMICS; POLARITY; LINE AB Back.-round: Continuous high spatial resolution observations of living A6 cells would greatly aid the elucidation of the relationship between structure and function and facilitate the study of major physiological processes Such as the mechanism of action of aldosterone. Unfortunately, observing the micro-structural and functional changes in the membrane of living cells is still a formidable challenge for a microscopist. Method: Scanning ion conductance microscopy (SICM), which uses a glass nanopipette as a sensitive probe, has been shown to be suitable for imaging non-conducting! surfaces bathed in electrolytes. A specialized version of this microscopy has been developed by our group and has been applied to image live cells at high-resolution for the first time. This method can also be used in conjunction with patch clamping to study both anatomy and function and identify ion channels in single cells. Results: This new microscopy provides high-resolution images of living renal cells which are comparable with those obtained by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Continuous 24 It observations under normal physiological conditions showed how A6 kidney epithelial cells changed their height, volume, and reshaped their borders. The changes in cell area correlated with the density of microvilli on the surface. Surface microvilli density ranged from 0.5 mum(-2) for extended cells to 2.5 mum(2) for shrunk cells. Patch clamping of individual cells enabled anatomy and function to be correlated. Conclusions: Scanning ion conductance microscopy provides unique information about living cells that helps to understand cellular function. It has the potential to become a powerful tool for research on living renal cells. (C) 2003 Elsevier Ireland Ltd. All rights reserved. C1 Univ London Imperial Coll Sci Technol & Med, Div Med, MRC, Ctr Clin Sci, London W12 0NN, England. Natl Inst Deafness & Other Commun Disorders, NIH, Bethesda, MD 20850 USA. USN, Res Off, Arlington, VA 22217 USA. Univ Newcastle Upon Tyne, Off Vice Chancellor, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England. Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England. RP Korchev, YE (reprint author), Univ London Imperial Coll Sci Technol & Med, Div Med, MRC, Ctr Clin Sci, Hammersmith Hosp Campus,Cane Rd, London W12 0NN, England. EM y.korchev@ic.ac.uk RI Sanchez, Daniel/B-5141-2009; Korchev, Yuri/D-2498-2009; OI Korchev, Yuri/0000-0002-4872-8696; Klenerman, David/0000-0001-7116-6954; Frolenkov, Gregory/0000-0002-9810-5024 FU Biotechnology and Biological Sciences Research Council [C19021] NR 25 TC 43 Z9 49 U1 2 U2 19 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD MAR 31 PY 2004 VL 217 IS 1-2 BP 101 EP 108 DI 10.1016/j.mce.2003.10.015 PG 8 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 823TG UT WOS:000221635500014 PM 15134807 ER PT J AU Vu, ND Feng, HQ Bai, YW AF Vu, ND Feng, HQ Bai, YW TI The folding pathway of barnase: The rate-limiting transition state and a hidden intermediate under native conditions SO BIOCHEMISTRY LA English DT Article ID PROTEIN ENGINEERING PROCEDURE; HELIX-FORMING TENDENCIES; HYDROGEN-EXCHANGE; AMINO-ACIDS; STRUCTURAL-CHARACTERIZATION; AMIDE-PROTON; CYTOCHROME-C; NMR; EQUILIBRIUM; STABILITY AB The nature of the rate-limiting transition state at zero denaturant (TS1) and whether there are hidden intermediates are the two major unsolved problems in defining the folding pathway of barnase. In earlier studies, it was shown that TS1 has small phi values throughout the structure of the protein, suggesting that the transition state has either a defined partially folded secondary structure with all side chains significantly exposed or numerous different partially unfolded structures with similar stability. To distinguish the two possibilities, we studied the effect of Gly mutations on the folding rate of barnase to investigate the secondary structure formation in the transition state. Two mutations in the same region of a beta-strand decreased the folding rate by 20- and 50-fold, respectively, suggesting that the secondary structures in this region are dominantly formed in the rate-limiting transition state. We also performed native-state hydrogen exchange experiments on barnase at pD 5.0 and 25 degreesC and identified a partially unfolded state. The structure of the intermediate was investigated using protein engineering and NMR. The results suggest that the intermediate has an omega loop unfolded. This intermediate is more folded than the rate-limiting transition state previously characterized at high denaturant concentrations (TS2). Therefore, it exists after TS2 in folding. Consistent with this conclusion, the intermediate folds with the same rate and denaturant dependence as the wild-type protein, but unfolds faster with less dependence on the denaturant concentration. These and other results in the literature suggest that barnase folds through partially unfolded intermediates that exist after the rate-limiting step. Such folding behavior is similar to those of cytochrome c and Rdapocyt b(562). Together, we suggest that other small apparently two-state proteins may also fold through hidden intermediates. C1 NCI, Biochem Lab, NIH, Bethesda, MD 20892 USA. RP Bai, YW (reprint author), NCI, Biochem Lab, NIH, Bldg 37,Room 6114E, Bethesda, MD 20892 USA. EM yawen@helix.nih.gov NR 55 TC 31 Z9 31 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 30 PY 2004 VL 43 IS 12 BP 3346 EP 3356 DI 10.1021/bi0362267 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 806OP UT WOS:000220443500006 PM 15035606 ER PT J AU Corley, SD Epstein, AE DiMarco, JP Domanski, MJ Geller, N Greene, HL Josephson, RA Kellen, JC Klein, RC Krahn, AD Mickel, M Mitchell, LB Nelson, JD Rosenberg, Y Schron, E Shemanski, L Waldo, AL Wyse, DG AF Corley, SD Epstein, AE DiMarco, JP Domanski, MJ Geller, N Greene, HL Josephson, RA Kellen, JC Klein, RC Krahn, AD Mickel, M Mitchell, LB Nelson, JD Rosenberg, Y Schron, E Shemanski, L Waldo, AL Wyse, DG CA AFFIRM Investigators TI Relationships between sinus rhythm, treatment, and survival in the atrial fibrillation follow-up investigation of rhythm management (AFFIRM) study SO CIRCULATION LA English DT Article DE antiarrhythmia agents; anticoagulants; arrhythmia; fibrillation ID ARRHYTHMIA SUPPRESSION TRIAL; MYOCARDIAL-INFARCTION; MORTALITY; MORBIDITY; EFFICACY; OUTCOMES; THERAPY AB Background - The AFFIRM Study showed that treatment of patients with atrial fibrillation and a high risk for stroke or death with a rhythm-control strategy offered no survival advantage over a rate-control strategy in an intention-to-treat analysis. This article reports an "on-treatment" analysis of the relationship of survival to cardiac rhythm and treatment as they changed over time. Methods and Results - Modeling techniques were used to determine the relationships among survival, baseline clinical variables, and time-dependent variables. The following baseline variables were significantly associated with an increased risk of death: increasing age, coronary artery disease, congestive heart failure, diabetes, stroke or transient ischemic attack, smoking, left ventricular dysfunction, and mitral regurgitation. Among the time-dependent variables, the presence of sinus rhythm (SR) was associated with a lower risk of death, as was warfarin use. Antiarrhythmic drugs (AADs) were associated with increased mortality only after adjustment for the presence of SR. Consistent with the original intention-to-treat analysis, AADs were no longer associated with mortality when SR was removed from the model. Conclusions - Warfarin use improves survival. SR is either an important determinant of survival or a marker for other factors associated with survival that were not recorded, determined, or included in the survival model. Currently available AADs are not associated with improved survival, which suggests that any beneficial antiarrhythmic effects of AADs are offset by their adverse effects. If an effective method for maintaining SR with fewer adverse effects were available, it might be beneficial. C1 Univ Alabama, Dept Med, Div Cardiovasc Dis, Birmingham, AL 35294 USA. Axio Res Corp, Seattle, WA USA. Univ Virginia, Charlottesville, VA USA. NHLBI, NIH, Bethesda, MD 20892 USA. Akron Cardiol Consultants, Akron, OH USA. Univ Calgary, Calgary, AB, Canada. Univ Utah, Med Ctr, Salt Lake City, UT USA. London Hlth Sci Ctr, London, ON, Canada. Univ Hosp Cleveland, Cleveland, OH 44106 USA. RP Epstein, AE (reprint author), Univ Alabama, Dept Med, Div Cardiovasc Dis, Tinsley Harrison Tower 321L,1530 3rd Ave S, Birmingham, AL 35294 USA. EM aepstein@uab.edu FU NHLBI NIH HHS [N01-HC-55139] NR 15 TC 601 Z9 622 U1 1 U2 13 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD MAR 30 PY 2004 VL 109 IS 12 BP 1509 EP 1513 DI 10.1161/01.CIR.0000121736.16643.11 PG 5 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 807QF UT WOS:000220515300014 PM 15007003 ER PT J AU Briard, E Pike, VW AF Briard, E Pike, VW TI Substitution-reduction: an alternative process for the [F-18]N-(2-fluoroethylation) of anilines SO JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS LA English DT Article DE fluorine-18; [F-18]N-(2-fluoroethylation); anifines; N-haloacetyl-anilines; nucleophilic substitution; reduction ID POSITRON-EMISSION-TOMOGRAPHY; SUPPORTED NUCLEOPHILIC-SUBSTITUTION; NO-CARRIER; SYNTHETIC PRECURSOR; F-18 FLUORIDE; AB-INITIO; PET; BROMIDE; FLUOROALKYLATION; DERIVATIVES AB Substitution of a halo atom (chloro or bromo) in easily prepared N-haloacetyl-anilines with no-carrier added (NCA) cyclotron-produced [F-18]fluoride ion (F-18, t(1/2) = 109.8 min; beta(+) = 96.9%), followed by reduction with borane-tetrahydrofuran (BH3-THF), provides an alternative route to NCA [F-18]N-(2-fluoroethyl)-anilines. This two-step and one-pot process is rapid (similar to50 min) and moderately high yielding (similar to 40% decay-corrected radiochemical yield (RCY) overall). In the nucleophilic substitution reaction, 18-crown-6 is preferred to Kryptofix(R) 222 as complexing agent for the solubilization of the counter-ion (K+), derived from an added metal salt, in acetonitrile. Weakly basic potassium bicarbonate is preferred as the added metal salt. Inclusion of a small amount of water, equating to 4-5 molar equivalents relative to 18-crown-6, base or precursor (held in equimolar ratio), is beneficial in preventing the adsorption of radioactivity onto the wall of the glass reaction vessel and for achieving high RCY in the nucleophilic substitution reaction. BH3-THF is effective for the rapid reduction of the generated [F-18]N-fluoroacetyl-aniline to the [F-18]N-(2-fluoroethyl)-aniline. Copyright (C) 2004 John Wiley Sons, Ltd. C1 Natl Inst Mental Hlth, Mol Imaging Branch, NIH, Bethesda, MD 20892 USA. RP Briard, E (reprint author), Natl Inst Mental Hlth, Mol Imaging Branch, NIH, Bldg 10,Room B3 C346,10 Ctr Dr, Bethesda, MD 20892 USA. EM briarde@intra.nimh.nih.gov NR 55 TC 20 Z9 20 U1 0 U2 6 PU JOHN WILEY & SONS LTD PI CHICHESTER PA THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND SN 0362-4803 J9 J LABELLED COMPD RAD JI J. Label. Compd. Radiopharm. PD MAR 30 PY 2004 VL 47 IS 4 BP 217 EP 232 DI 10.1002/jlcr.816 PG 16 WC Biochemical Research Methods; Chemistry, Medicinal; Chemistry, Analytical SC Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry GA 817LL UT WOS:000221178900002 ER PT J AU Yotsu-Yamashita, M Kim, YH Dudley, SC Choudhary, G Pfahnl, A Oshima, Y Daly, JW AF Yotsu-Yamashita, M Kim, YH Dudley, SC Choudhary, G Pfahnl, A Oshima, Y Daly, JW TI The structure of zetekitoxin AB, a saxitoxin analog from the Panamanian golden frog Atelopus zeteki: A potent sodium-channel blocker SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PARALYTIC SHELLFISH TOXINS; GENUS ATELOPUS; TETRODOTOXIN; SKIN; ATELOPIDTOXIN; NEOSAXITOXIN; WATER; DINOFLAGELLATE; SITE AB Bufonid anurans of the genus Atelopus contain both steroidal bufadienolides and various guanidinium alkaloids of the tetrodotoxin class. The former inhibit sodium-potassium ATPases, whereas the latter block voltage-dependent sodium channels. The structure of one guanidinium alkaloid, zetekitoxin AB, has remained a mystery for over 30 years. The structure of this alkaloid now has been investigated with a sample of approximate to0.3 mg, purified from extracts obtained decades ago from the Panamanian golden frog Atelopus zeteki. Detailed NMR and mass spectral analyses have provided the structure and relative stereochemistry of zetekitoxin AB and have revealed that it is an analog of saxitoxin. The proposed structure is characterized by richness of heteroatoms (C16H25N8O12S) and contains a unique 1,2-oxazolidine ring-fused lactam, a sulfate ester, and an N-hydroxycarbamate moiety. Zetekitoxin AB proved to be an extremely potent blocker of voltage-dependent sodium channels expressed in Xenopus oocytes. The IC50 values were 280 pM for human heart channels, 6.1 pM for rat brain IIa channels, and 65 pM for rat skeletal muscle channels, thus being roughly 580-, 160-, and 63-fold more potent at these channels than saxitoxin. C1 Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan. Korea Adv Inst Sci & Technol, Dept Chem, Ctr Mol Design & Synth, Taejon 305701, South Korea. Emory Univ, Dept Med, Atlanta, GA 30322 USA. Atlanta Vet Affairs Med Ctr, Decatur, GA 30033 USA. Tohoku Univ, Grad Sch Life Sci, Sendai, Miyagi 9818555, Japan. NIDDKD, Bioorgan Chem Lab, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Yotsu-Yamashita, M (reprint author), Tohoku Univ, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan. EM myama@biochem.tohoku.ac.jp; kyh@kaist.ac.kr FU NHLBI NIH HHS [HL64828, R01 HL064828] NR 37 TC 49 Z9 52 U1 1 U2 15 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 2004 VL 101 IS 13 BP 4346 EP 4351 DI 10.1073/pnas.0400368101 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 809PN UT WOS:000220648700006 PM 15070720 ER PT J AU Huh, CG Factor, VM Sanchez, A Uchida, K Conner, EA Thorgeirsson, SS AF Huh, CG Factor, VM Sanchez, A Uchida, K Conner, EA Thorgeirsson, SS TI Hepatocyte growth factor/c-met signaling pathway is required for efficient liver regeneration and repair SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PROTEIN-TYROSINE KINASE; SCATTER FACTOR-RECEPTOR; INVASIVE GROWTH; SEMAPHORIN RECEPTORS; MICE; OSTEOPONTIN; CELLS; GENE; FAS; HGF/SF AB Hepatocyte growth factor/scatter factor c-met signaling pathway is of central importance during development as well as in tumorigenesis. Because homozygous null mice for either hgf/sf or c-met die in utero, we used Cre/loxP-mediated gene targeting to investigate the function of c-met specifically in the adult liver. Loss of c-met appeared not to be detrimental to hepatocyte function under physiological conditions. Nonetheless, the adaptive responses of the liver to injury were dramatically affected. Mice lacking c-met gene in hepatocytes were hypersensitive to Fas-induced apoptosis. When injected with a low dose of anti-Fas antibody, the majority of these mice died from massive apoptosis and hemorrhagic necrosis, whereas all wild-type mice survived with signs of minor injury. After a challenge with a single necrogenic dose of CCl4, c-met conditional knockout mice exhibited impaired recovery from centrolobular lesions rather than a deficit in hepatocyte proliferation. The delayed healing was associated with a persistent inflammatory reaction, overproduction of osteopontin, early and prominent dystrophic calcification, and impaired hepatocyte scattering/migration into diseased areas. These studies provide direct genetic evidence in support of the critical role of c-met in efficient liver regeneration and suggest that disruption of c-met affects primarily hepatocyte survival and tissue remodeling. C1 NCI, Expt Carcinogenesis Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA. RP Thorgeirsson, SS (reprint author), NCI, Expt Carcinogenesis Lab, Ctr Canc Res, NIH, 37 Convent Dr MSC 4262,Bldg 37,Room 4146A, Bethesda, MD 20892 USA. EM snorri_thorgeirsson@nih.gov RI Sanchez, Aranzazu/H-7810-2015 OI Sanchez, Aranzazu/0000-0001-9145-6633 NR 41 TC 416 Z9 440 U1 1 U2 26 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 2004 VL 101 IS 13 BP 4477 EP 4482 DI 10.1073/pnas.0306068101 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 809PN UT WOS:000220648700029 PM 15070743 ER PT J AU Treusch, S Knuth, S Slaugenhaupt, SA Goldin, E Grant, BD Fares, H AF Treusch, S Knuth, S Slaugenhaupt, SA Goldin, E Grant, BD Fares, H TI Caenorhabditis elegans functional orthologue of human protein h-mucolipin-1 is required for lysosome biogenesis SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE mucolipidosis type IV; CUP-5 ID MUCOLIPIDOSIS TYPE-IV; CELL-DEATH; FLUORESCENT PROTEIN; ABNORMAL TRANSPORT; ENDOCYTIC PATHWAY; FUSION; GENE; IDENTIFICATION; MUTATIONS; ENDOSOMES AB Mucolipidosis type IV (MLIV) is an autosomal recessive lysosomal storage disease characterized by severe psychomotor retardation, achlorhydria, and ophthalmological abnormalities. Cells from several tissues in MLIV patients accumulate large vacuoles that are presumed to be lysosomes, but whose exact nature remains to be determined. Other defects include the deterioration of neuronal integrity in the retina and the cerebellum. MCOLN1, the gene mutated in MLIV patients, encodes a protein called h-mucolipin-1 that has six predicted transmembrane domains and functions as a Ca2+-permeable channel that is modulated by changes in Ca2+ concentration. CUP-5 is the Caenorhabditis elegans functional orthologue of h-mucolipin-1. Mutations in cup-5 result in the accumulation of large vacuoles in several cells, in increased cell death, and in embryonic lethality. We demonstrate here that CUP-5 functions in the biogenesis of lysosomes originating from hybrid organelles. We also show that at least two h-mucolipin family members rescue cup-5 mutant endocytic defects, indicating that there may be functional redundancy among the human proteins. Finally, we propose a model that relates the lysosome biogenesis defect in the absence of CUP-5/h-mucolipin-1 to cellular phenotypes in worms and in humans. C1 Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA. Harvard Univ, Sch Med, Harvard Inst Human Genet, Boston, MA 02115 USA. NINDS, Dev & Metab Neurol Branch, NIH, Bethesda, MD 20892 USA. Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08854 USA. RP Fares, H (reprint author), Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA. EM fares@email.arizona.edu OI Grant, Barth/0000-0002-5943-8336 FU NIGMS NIH HHS [R01 GM067237, R01 GM067237-01] NR 48 TC 151 Z9 154 U1 1 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 2004 VL 101 IS 13 BP 4483 EP 4488 DI 10.1073/pnas.0400709101 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 809PN UT WOS:000220648700030 PM 15070744 ER PT J AU Wyatt, LS Earl, PL Eller, LA Moss, B AF Wyatt, LS Earl, PL Eller, LA Moss, B TI Highly attenuated smallpox vaccine protects mice with and without immune deficiencies against pathogenic vaccinia virus challenge SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID HOST-RANGE; T-CELLS; CD8-T-CELL MEMORY; CD4-T-CELL HELP; ANKARA STRAIN; MVA STRAIN; INFECTION; BETA-2-MICROGLOBULIN; IMMUNOGENICITY; REPLICATION AB Modified vaccinia virus Ankara (MVA), developed > 30 years ago as a highly attenuated candidate smallpox vaccine, was recloned from a 1974 passage and evaluated for safety and immunogenicity. Replication of MVA is impaired in most mammalian cells, and we found that mice with severe combined immunodeficiency disease remained healthy when inoculated with MVA at 1,000 times the lethal dose of vaccinia virus derived from the licensed Dryvax vaccine seed. In BALB/c mice inoculated intramuscularly with MVA, virus-specific CD8(+) T cells and antibodies to purified virions and membrane protein components of the intracellular and extracellular infectious forms of vaccinia virus were induced in a dose-dependent manner. After one or two inoculations of MVA, the T cell numbers and antibody titers equaled or exceeded those induced by percutaneous injection of Dryvax. Antibodies induced by MVA and Dryvax were neutralizing and inhibited virus spread in cultured cells. Furthermore, vaccinated mice were protected against lethal intranasal challenge with a pathogenic vaccinia virus. B cell-deficient mice unable to generate antibodies and beta(2)-microglobulin-deficient mice unable to express MHC class I molecules for a CD8(+) T cell response were also protectively vaccinated by MVA. In contrast, mice with decreased CD4 or MHC class II expression and double-knockout mice deficient in MHC class land II-restricted activities were poorly protected or unprotected. This study confirmed the safety of MVA and demonstrated that the overlapping immune responses protected normal and partially immune-deficient animals, an encouraging result for this candidate attenuated smallpox vaccine. C1 NIAID, Viral Dis Lab, Bethesda, MD 20892 USA. Henry M Jackson Fdn, Rockville, MD 20850 USA. RP Moss, B (reprint author), NIAID, Viral Dis Lab, Bethesda, MD 20892 USA. EM bmoss@niaid.nih.gov NR 44 TC 164 Z9 164 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 2004 VL 101 IS 13 BP 4590 EP 4595 DI 10.1073/pnas.0401165101 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 809PN UT WOS:000220648700048 PM 15070762 ER PT J AU Matrosovich, MN Matrosovich, TY Gray, T Roberts, NA Klenk, HD AF Matrosovich, MN Matrosovich, TY Gray, T Roberts, NA Klenk, HD TI Human and avian influenza viruses target different cell types in cultures of human airway epithelium SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID A VIRUSES; RECEPTOR SPECIFICITY; HOST-CELL; INFECTION; EVOLUTION; STRAINS; H2 AB The recent human infections caused by H5N1, H9N2, and H7N7 avian influenza viruses highlighted the continuous threat of new pathogenic influenza viruses emerging from a natural reservoir in birds. It is generally believed that replication of avian influenza viruses in humans is restricted by a poor fit of these viruses to cellular receptors and extracellular inhibitors in the human respiratory tract. However, detailed mechanisms of this restriction remain obscure. Here, using cultures of differentiated human airway epithelial cells, we demonstrated that influenza viruses enter the airway epithelium through specific target cells and that there were striking differences in this respect between human and avian viruses. During the course of a single-cycle infection, human viruses preferentially infected nonciliated cells, whereas avian viruses as well as the egg-adapted human virus variant with an avian virus-like receptor specificity mainly infected ciliated cells. This pattern correlated with the predominant localization of receptors for human viruses (2-6-linked sialic acids) on nonciliated cells and of receptors for avian viruses (2-3-linked sialic acids) on ciliated cells. These findings suggest that although avian influenza viruses can infect human airway epithelium, their replication may be limited by a nonoptimal cellular tropism. Our data throw light on the mechanisms of generation of pandemic viruses from their avian progenitors and open avenues for cell level-oriented studies on the replication and pathogenicity of influenza virus in humans. C1 Univ Marburg, Inst Virol, D-35037 Marburg, Germany. MP Chumakov Inst Poliomyelitis & Viral Encephalit, Moscow 142782, Russia. NIEHS, Mol Carcinogenesis Lab, Res Triangle Pk, NC 27709 USA. Roche Prod Ltd, Welwyn Garden City AL7 3AY, Herts, England. RP Matrosovich, MN (reprint author), Univ Marburg, Inst Virol, D-35037 Marburg, Germany. EM mikhail.matrosovich@med.uni-marburg.de NR 31 TC 417 Z9 462 U1 5 U2 41 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 2004 VL 101 IS 13 BP 4620 EP 4624 DI 10.1073/pnas.0308001101 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 809PN UT WOS:000220648700053 PM 15070767 ER PT J AU Berkower, I Raymond, M Muller, J Spadaccini, A Aberdeen, A AF Berkower, I Raymond, M Muller, J Spadaccini, A Aberdeen, A TI Assembly, structure, and antigenic properties of virus-like particles rich in HIV-1 envelope gp120 SO VIROLOGY LA English DT Article DE virus-like particles; HIV-1; envelope glycoprotein gp120 ID HUMAN-IMMUNODEFICIENCY-VIRUS; B SURFACE-ANTIGEN; HUMAN MONOCLONAL-ANTIBODY; NEUTRALIZING ANTIBODIES; TYPE-1 PARTICLES; GLYCOPROTEIN; EPITOPE; PROTEIN; VACCINE; BINDING AB In order to improve the immunogenicity of HIV-1 envelope glycoproteins, we have fused gp120 to a carrier protein, hepatitis B surface antigen (HBsAg), which is capable of spontaneous assembly into virus-like particles. The HBsAg-gp120 hybrid proteins assembled efficiently into 20-30 nm particles. The particles resemble native HBsAg particles in size and density, consistent with a lipid composition of about 25% and a gp120 content of about 100 per particle. Particulate gp120 folds in its native conformation and is biologically active, as shown by high affinity binding of CD4. The particles express conformational determinants targeted by a panel of broadly cross-reactive neutralizing antibodies, and they show tight packing of gp120. Because the particles are lipoprotein micelles, an array of gp120 on their surface closely mimics gp120 on the surface of HIV-1 virions. These gp120-rich particles can enhance the quality, as well as quantity, of antibodies elicited by a gp120 vaccine. Published by Elsevier Inc. C1 Off Vaccine Res & Review, Ctr Biol, Div Viral Prod, Lab Immunoregulat, Bethesda, MD 20892 USA. RP Berkower, I (reprint author), Off Vaccine Res & Review, Ctr Biol, Div Viral Prod, Lab Immunoregulat, FDA,Bldg 29,Room 523,NIH Campus, Bethesda, MD 20892 USA. EM Berkower@cber.fda.gov NR 63 TC 23 Z9 24 U1 0 U2 3 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAR 30 PY 2004 VL 321 IS 1 BP 75 EP 86 DI 10.1016/j.virol.2003.12.017 PG 12 WC Virology SC Virology GA 807KB UT WOS:000220499300009 PM 15033567 ER PT J AU Snapp, EL Reinhart, GA Bogert, BA Lippincott-Schwartz, J Hegde, RS AF Snapp, EL Reinhart, GA Bogert, BA Lippincott-Schwartz, J Hegde, RS TI The organization of engaged and quiescent translocons in the endoplasmic reticulum of mammalian cells SO JOURNAL OF CELL BIOLOGY LA English DT Article DE FRET; TRAP; protein translocation; Sec61; TRAM ID PROTEIN-CONDUCTING CHANNEL; RESONANCE ENERGY-TRANSFER; SIGNAL SEQUENCE RECOGNITION; ER MEMBRANE; SECRETORY PROTEINS; RIBOSOMES; COMPLEX; TRANSPORT; ANGSTROM; PORE AB Protein translocons of the mammalian endoplasmic reticulum are composed of numerous functional components whose organization during different stages of the transport cycle in vivo remains poorly understood. We have developed generally applicable methods based on fluorescence resonance energy transfer (FRET) to probe the relative proximities of endogenously expressed translocon components in cells. Examination of substrate-engaged translocons revealed oligomeric assemblies of the Sec61 complex that were associated to varying degrees with other essential components including the signal recognition particle receptor TRAM and the TRAP complex. Remarkably, these components not only remained assembled but also had a similar, yet distinguishable, organization both during and after nascent chain translocation. The persistence of preassembled and complete translocons between successive rounds of transport may facilitate highly efficient translocation in vivo despite temporal constraints imposed by ongoing translation and a crowded cellular environment. C1 NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. RP Hegde, RS (reprint author), NICHHD, Cell Biol & Metab Branch, NIH, 18 Lib Dr,Bldg 18,Rm 101, Bethesda, MD 20892 USA. EM hegder@mail.nih.gov OI Hegde, Ramanujan/0000-0001-8338-852X NR 35 TC 50 Z9 50 U1 1 U2 2 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA SN 0021-9525 J9 J CELL BIOL JI J. Cell Biol. PD MAR 29 PY 2004 VL 164 IS 7 BP 997 EP 1007 DI 10.1083/jcb.200312079 PG 11 WC Cell Biology SC Cell Biology GA 808NP UT WOS:000220576100007 PM 15051734 ER PT J AU Leitner, WW Hwang, LN Bergmann-Leitner, ES Finkelstein, SE Frank, S Restifo, NP AF Leitner, WW Hwang, LN Bergmann-Leitner, ES Finkelstein, SE Frank, S Restifo, NP TI Apoptosis is essential for the increased efficacy of alphaviral replicase-based DNA vaccines SO VACCINE LA English DT Article DE DNA vaccines; alphavirus; apoptosis; BCl-X-L ID HERPES-SIMPLEX VIRUS; HUMAN-MELANOMA CELLS; DENDRITIC CELLS; TUMOR-CELLS; ANTIVIRAL PATHWAYS; MEDIATED APOPTOSIS; EXPRESSION VECTORS; IMMUNE-RESPONSES; GENE-EXPRESSION; SELF-ANTIGEN AB Alphaviral replicons can increase the efficacy and immunogenicity of naked nucleic acid vaccines. To study the impact of apoptosis on this increased effectiveness, we co-delivered an anti-apoptotic gene (BCI-X-L) with the melanocyte/melanoma differentiation antigen TRP-1. Although cells co-transfected with Bcl-X-L lived longer, produced more antigen and elicited increased antibody production in vivo, co-delivery of pro-survival BCI-X-L with antigen significantly reduced the ability of the replicase-based vaccine to protect against an aggressive tumor challenge. These data show for the first time that the induction of apoptotic cell death of transfected cells in vivo is required for the increased effectiveness of replicase-based vaccines. Our findings also provide an explanation for the paradoxical observation that replicase-based DNA vaccines are much more immunogenic than conventional constructs despite reduced antigen production. Published by Elsevier Ltd. C1 Natl Canc Inst, Surg Branch, Natl Inst Hlth, Bethesda, MD 20892 USA. Walter Reed Army Inst Res, Dept Immunol, Silver Spring, MD 20910 USA. Univ Bonn, Dept Neuropathol, D-53105 Bonn, Germany. RP Restifo, NP (reprint author), Natl Canc Inst, Surg Branch, Natl Inst Hlth, Bldg 10,Room 2B42, Bethesda, MD 20892 USA. EM wolfgang_leitner@nih.gov; restifo@nih.gov RI Restifo, Nicholas/A-5713-2008; Bergmann-Leitner, Elke/B-3548-2011; Leitner, Wolfgang/F-5741-2011; OI Bergmann-Leitner, Elke/0000-0002-8571-8956; Leitner, Wolfgang/0000-0003-3125-5922; Restifo, Nicholas P./0000-0003-4229-4580 FU Intramural NIH HHS [Z01 BC010763-01, Z99 CA999999] NR 46 TC 58 Z9 68 U1 0 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 29 PY 2004 VL 22 IS 11-12 BP 1537 EP 1544 DI 10.1016/j.vaccine.2003.10.013 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 812EB UT WOS:000220821700025 PM 15063579 ER PT J AU Anfinrud, PA Schotte, F Wulff, M AF Anfinrud, PA Schotte, F Wulff, M TI Watching proteins function with picosecond X-ray crystallography and MD simulations. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. European Synchrotron & Radiat Facil, Grenoble, France. EM anfinrud@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 478-PHYS BP U331 EP U331 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655701720 ER PT J AU Bear, S AF Bear, S TI Keeping up with the changing face of Medline and mesh - 3 keys to improving searches. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Natl Lib Med, NIH, Bethesda, MD 20894 USA. EM bears@mail.nlm.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 004-CINF BP U678 EP U678 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602468 ER PT J AU Benjers, BM AF Benjers, BM TI Chemical information in MEDLINE/PUBMED. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Natl Lib Med, Index Sect, Bethesda, MD 20894 USA. EM benjersb@mail.nim.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 044-CINF BP U684 EP U684 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602508 ER PT J AU Betz, JM AF Betz, JM TI Botanical quality initiatives at the office of dietary supplements, national institutes of health. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Off Dietary Supplements, NIH, Bethesda, MD 20892 USA. EM betzj@od.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 102-AGFD BP U44 EP U44 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655600110 ER PT J AU Choi, EJ Dimitriadis, EK AF Choi, EJ Dimitriadis, EK TI Cytochrome C adsorption to supported, anionic lipid bilayers studied via atomic force microscopy. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 ORS, Instrumentat & Res Dev Resource, Div Bioengn & Phys Sci, NIH, Bethesda, MD 20892 USA. EM choieung@ors.od.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 536-COLL BP U890 EP U890 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655603157 ER PT J AU Choi, HK Cho, S Lee, J Blumberg, PM AF Choi, HK Cho, S Lee, J Blumberg, PM TI Non-vanilloid resiniferatoxin analogues as vanilloid receptor 1 ligands. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Seoul Natl Univ, Coll Pharm, Med Chem Lab, Seoul 151742, South Korea. NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA. EM jeewoo@snu.ac.kr NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 241-MEDI BP U50 EP U50 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700239 ER PT J AU Chu, JW Brooks, BR Trout, B AF Chu, JW Brooks, BR Trout, B TI Combined OM/MM studies and free energy simulations on the oxidation of methionine residues in granulocyte colony stimulating factor. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 MIT, Dept Chem Engn, Cambridge, MA 02139 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 041-COMP BP U902 EP U902 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655603210 ER PT J AU Dimitriadis, EK Pascual, J Horkay, F AF Dimitriadis, EK Pascual, J Horkay, F TI Morphology of DNA adsorbed from solutions on hydrophobic surfaces. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIH, Div Bioengn & Phys Sci, OD, Bethesda, MD 20892 USA. NICHD, Lab Integrat & Med Biophys, NIH, Bethesda, MD 20892 USA. EM dimitria@helix.nih.gov; horkay@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 347-BIOT BP U244 EP U244 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655600899 ER PT J AU Duan, D Lai, CC Kelley, JA Lewin, NE Blumberg, PM Marquez, VE AF Duan, D Lai, CC Kelley, JA Lewin, NE Blumberg, PM Marquez, VE TI Combinatorial synthesis, quality control and biological evaluation of diacylglycerol (DAG)-lactone libraries - Protein kinase C (PKC)-isozyme-specific ligands. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Med Chem Lab, Ctr Canc Res, Bethesda, MD 20892 USA. NIH, Lab Cellular Carcinogenesis & Tumor Promot, Div Basic Sci, NIH, Bethesda, MD 20892 USA. NCI, Med Chem Lab, NIH, Ft Detrick, MD 21702 USA. EM duand@ncifcrf.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 033-MEDI BP U10 EP U10 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700033 ER PT J AU Eckstrand, IA AF Eckstrand, IA TI Bridges to the future. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIGMS, Minor Opportun Res Div, NIH, Bethesda, MD 20892 USA. EM Irene_Eckstrand@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 647-CHED BP U497 EP U497 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655601923 ER PT J AU Ekstrom, D Cheng, W Andersson, R Mitra, G Zhu, JW AF Ekstrom, D Cheng, W Andersson, R Mitra, G Zhu, JW TI Adenovirus production and recovery using a wave bioreactor. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 SAIC Frederick, Biopharmaceut Dev Program, NCI FCRDC, Frederick, MD 21702 USA. EM dekstrom@ncifcrf.gov NR 0 TC 2 Z9 2 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 097-BIOT BP U202 EP U202 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655600651 ER PT J AU Etzler, K AF Etzler, K TI NIH's SBIR/STTR: Funding programs for technology based businesses. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIH, SBIR STTR Program, Off External Programs, Bethesda, MD 20892 USA. EM EtzlerK@OD.NIH.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 4-SCHB BP U571 EP U571 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655702946 ER PT J AU Gopich, I Szabo, A AF Gopich, I Szabo, A TI Theory of FRET distributions from single-molecule experiments SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. EM gopich@nih.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 383-PHYS BP U317 EP U317 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655701625 ER PT J AU Hirai, T Heymann, JAW Shi, D Subramaniam, S AF Hirai, T Heymann, JAW Shi, D Subramaniam, S TI Structure and transport mechanism in the Major Facilitator Superfamily of 12-helix membrane proteins. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, NIH, Bethesda, MD 20892 USA. EM teru@nih.gov RI Hirai, Teruhisa/G-2105-2015 OI Hirai, Teruhisa/0000-0002-2114-8149 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 004-COLL BP U804 EP U804 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602626 ER PT J AU Horkay, F Fleury, C Horkayne-Szakaly, I Basser, PJ AF Horkay, F Fleury, C Horkayne-Szakaly, I Basser, PJ TI Swelling measurements on cartilage/hydrogel constructs. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NICHD, Lab Integrat & Med Biophys, NIH, Bethesda, MD 20892 USA. EM horkay@helix.nih.gov RI Basser, Peter/H-5477-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 339-BIOT BP U242 EP U242 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655600891 ER PT J AU Horkay, F Basser, PJ Hecht, AM Geissler, E AF Horkay, F Basser, PJ Hecht, AM Geissler, E TI Osmotic behavior of DNA gels swollen in physiological salt solutions. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NICHD, Lab Integrat & Med Biophys, NIH, Bethesda, MD 20892 USA. Univ Grenoble 1, CNRS, UMR 5588, Spectrometrie Phys Lab, F-38041 Grenoble, France. EM horkay@helix.nih.gov RI Basser, Peter/H-5477-2011 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 196-PMSE BP U519 EP U519 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655702648 ER PT J AU Hummer, G Szabo, A AF Hummer, G Szabo, A TI Thermodynamics and kinetics from nonequilibrium single molecule pulling experiments. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. EM Gerhard.Hummer@nih.gov RI Hummer, Gerhard/A-2546-2013 OI Hummer, Gerhard/0000-0001-7768-746X NR 0 TC 1 Z9 1 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 014-COMP BP U897 EP U897 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655603183 ER PT J AU Ioanoviciu, AS Antony, S Pommier, Y Cushman, M AF Ioanoviciu, AS Antony, S Pommier, Y Cushman, M TI Synthesis of new indenoisoquinolines: Cytotoxic agents and topoisomerase I inhibitors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA. NCI, Mol Pharmacol Lab, Bethesda, MD 20892 USA. EM aioanovi@purdue.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 116-MEDI BP U27 EP U27 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700115 ER PT J AU Jacobson, KA Kim, SK Costanzi, S AF Jacobson, KA Kim, SK Costanzi, S TI Molecular modeling of purine and pyrimidine G protein-coupled receptors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. EM kajacobs@helix.nih.gov RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 067-COMP BP U906 EP U906 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655603235 ER PT J AU Jacobson, KA Kim, SK Ohno, M Duong, HT Van Rompaey, P Van Calenbergh, S Gao, ZG AF Jacobson, KA Kim, SK Ohno, M Duong, HT Van Rompaey, P Van Calenbergh, S Gao, ZG TI Neoceptor concept applied to human A2A and A3 adenosine receptors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. Lab Med Chem FFW, B-9000 Ghent, Belgium. EM kajacobs@helix.nih.gov RI Van Calenbergh, Serge/A-3167-2008; Jacobson, Kenneth/A-1530-2009 OI Van Calenbergh, Serge/0000-0002-4201-1264; Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 208-MEDI BP U44 EP U44 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700206 ER PT J AU Jeong, LS Shin, DH Kim, HO Jung, JY Kim, HJ Kim, WK Melman, N Gao, ZG Jacobson, KA AF Jeong, LS Shin, DH Kim, HO Jung, JY Kim, HJ Kim, WK Melman, N Gao, ZG Jacobson, KA TI Structure-activity relationship of N6-substituted D-4 '-thioadenosine derivatives as potent and selective agonists at the human A3 adenosine receptor. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Ewha Womans Univ, Coll Pharm, Med Chem Lab, Seoul, South Korea. NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. EM lakjeong@mm.ewha.ac.kr RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 258-MEDI BP U54 EP U54 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700256 ER PT J AU Jin, MK Yoon, HS Kang, SU Lee, J Ha, HJ Kim, YH Blumberg, PM AF Jin, MK Yoon, HS Kang, SU Lee, J Ha, HJ Kim, YH Blumberg, PM TI Alpha-substituted N-(4-T-butylbenzyl)-N '-[4(methylsulfonylamino)benzyl]thiourea analogues as vanilloid receptor antagonists. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Seoul Natl Univ, Coll Pharm, Med Chem Lab, Seoul 151742, South Korea. Digital Biotech, Ansan, South Korea. NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA. EM jeewoo@snu.ac.kr NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 239-MEDI BP U50 EP U50 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700237 ER PT J AU Joshi, BV Marquez, VE Fettinger, JC Jacobson, KA AF Joshi, BV Marquez, VE Fettinger, JC Jacobson, KA TI New synthetic route to (N)methanocarba nucleosides acting as potent A3 adenosine receptor agonists. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. NCI, Med Chem Lab, CCR, NIH, Bethesda, MD 20892 USA. Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA. EM BalachandraJ@intra.niddk.nih.gov RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 256-MEDI BP U53 EP U53 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700254 ER PT J AU Kang, JH Peach, ML Blumberg, PM Marquez, VE AF Kang, JH Peach, ML Blumberg, PM Marquez, VE TI New design approaches aimed at targeting atypical PKC isoform zeta with diacylglycerol-lactones (DAG-lactones). SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Med Chem Lab, Ctr Canc Res, NIH, Frederick, MD 21702 USA. NCI, Lab Cellular Carcinogenesis & Tumor Promot, Div Basic Sci, NIH, Frederick, MD 21702 USA. EM jhkang@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 031-MEDI BP U10 EP U10 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700031 ER PT J AU Kang, S Shi, ZD Lee, K Worthy, KM Fisher, RJ Burke, TR AF Kang, S Shi, ZD Lee, K Worthy, KM Fisher, RJ Burke, TR TI Examination of the role of phosphoryl-mimicking functionality in the binding of picomolar-affinity Grb2 SH2 domain-binding macrocycles. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Med Chem Lab, Ctr Canc Res, NIH, Frederick, MD 21702 USA. EM serforchr@hanmail.net RI Fisher, Robert/B-1431-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 041-MEDI BP U12 EP U12 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700041 ER PT J AU Karki, RG Nicklaus, MC AF Karki, RG Nicklaus, MC TI Understanding the integrase inhibitory activity of azido containing HIV-1 integrase inhibitors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Med Chem Lab, NIH, Frederick, MD 21702 USA. EM rajeshri@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 017-MEDI BP U7 EP U7 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700018 ER PT J AU Kim, YC Jung, KY Kim, SK Gao, ZG Gross, AS Melman, N Jacobson, KA AF Kim, YC Jung, KY Kim, SK Gao, ZG Gross, AS Melman, N Jacobson, KA TI Structure-activity relationships of thiazole and thiadiazole derivatives as potent and selective human adenosine A(3) receptor antagonists. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Kwangju Inst Sci & Technol, Dept Life Sci, Kwangju 500712, South Korea. NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. EM yongchul@kjist.ac.kr RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 255-MEDI BP U53 EP U53 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700253 ER PT J AU Lang, LX Jagoda, E Ma, Y Sassaman, M Eckelman, WC AF Lang, LX Jagoda, E Ma, Y Sassaman, M Eckelman, WC TI Synthesis and in vivo biodistribution of F-18 labeled 3-cis, 3-trans, 4-cis, and 4-trans fluorocyclohexane derivatives of WAY 100635 SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIH, PET Dept, Ctr Clin, Bethesda, MD 20892 USA. EM llang@mail.nih.gov; weckelman@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 222-ORGN BP U140 EP U140 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700731 ER PT J AU Lee, J Kang, JH Kim, Y Kim, SE Kim, YH Kim, H Blumberg, PM AF Lee, J Kang, JH Kim, Y Kim, SE Kim, YH Kim, H Blumberg, PM TI DAG-lactones as high-affinity protein kinase C ligands and alpha-secretase activators. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Seoul Natl Univ, Med Chem Lab, Coll Pharm, Seoul 151742, South Korea. NCI, Lab Cellular Carcinogenesis & Tumor Promot, NIH, Bethesda, MD 20892 USA. EM jeewoo@snu.ac.kr NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 034-MEDI BP U10 EP U11 PN 2 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700034 ER PT J AU Lee, JG Sagui, C Darden, T Roland, C AF Lee, JG Sagui, C Darden, T Roland, C TI Ab initio study of binding affinity of glycopeptide antibiotics to bacterial cell wall analogs. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 N Carolina State Univ, Dept Phys, Raleigh, NC 27695 USA. NIEHS, Struct Biol Lab, Res Triangle Pk, NC 27709 USA. EM jglee@nemo.physics.ncsu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 040-COMP BP U902 EP U902 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655603209 ER PT J AU Lu, Q Yang, YT Chen, CS Davis, M Byrd, JC Umar, A Chen, CS AF Lu, Q Yang, YT Chen, CS Davis, M Byrd, JC Umar, A Chen, CS TI Zn2+-chelating motif-tethered short-chain fatty acids as a novel class of histone deacetylase inhibitors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Ohio State Univ, Coll Pharm, Dept Med Chem, Columbus, OH 43210 USA. Ohio State Univ, Arthur James Comprehens Canc Ctr, Div Hematol Oncol, Columbus, OH 43210 USA. NCI, Canc Res Ctr, Lab Biosyst & Canc, Bethesda, MD 20892 USA. EM lu.159@osu.edu RI lu, qiang/C-7764-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 119-MEDI BP U27 EP U27 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700118 ER PT J AU Luzzio, FA Duveau, DY Mayorov, AV Figg, WD AF Luzzio, FA Duveau, DY Mayorov, AV Figg, WD TI Synthetic routes to metabolites and metabolic analogues of thalidomide: Refinements of the synthesis of 5 '-hydroxythalidomide and its acyclic derivatives. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Univ Louisville, Dept Chem, Louisville, KY 40292 USA. NCI, Canc Therapeut Branch, Bethesda, MD 20892 USA. EM d0duve0l@louisville.edu RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 335-MEDI BP U68 EP U69 PN 2 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700333 ER PT J AU May, S Harries, D Tzlil, S Ben-Shaul, A AF May, S Harries, D Tzlil, S Ben-Shaul, A TI Macroion adsorption on mixed fluid membranes, lipid demixing and domain formation. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Univ Jena, Inst Mol Biol, D-07745 Jena, Germany. NICHD, Lab Phys & Struct Biol, NIH, Bethesda, MD 20892 USA. Hebrew Univ Jerusalem, Dept Chem Phys, IL-91904 Jerusalem, Israel. Hebrew Univ Jerusalem, Fritz Haber Res Ctr, IL-91904 Jerusalem, Israel. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 088-COLL BP U817 EP U817 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602710 ER PT J AU Minton, AP AF Minton, AP TI Predicted and observed effects of excluded volume on the self-assembly of protein fibers and other complexes in crowded solutions. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, NIH, Bethesda, MD 20892 USA. EM minton@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 002-COLL BP U804 EP U804 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602624 ER PT J AU Nossal, R AF Nossal, R TI Regulating the assembly of clathrin baskets. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Natl Inst Child Hlth & Human Dev, NIH, LIMB, Bethesda, MD 20892 USA. EM nossalr@mail.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 040-COLL BP U809 EP U809 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602662 ER PT J AU Ohno, M Kim, HS Costanzi, S Kempeneers, V Vastmans, K Herdewijn, P Maddileti, S Harden, TK Jacobson, KA AF Ohno, M Kim, HS Costanzi, S Kempeneers, V Vastmans, K Herdewijn, P Maddileti, S Harden, TK Jacobson, KA TI Nucleotide analogues containing 2-oxa-bicyclo[2.2.1]heptane and L-alpha-threofuranosyl ring systems: Interactions with purine/pyrimidine receptors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. Wonkwang Univ, Coll Pharm, Chollabuk Do, South Korea. Wonkwang Univ, Med Resources Res Ctr, Chollabuk Do, South Korea. Katholieke Univ Leuven, Rega Inst Med Res, Chem Pharmacol Lab, Louvain, Belgium. Univ N Carolina, Sch Med, Chapel Hill, NC 27515 USA. EM michihiroo@intra.niddk.nih.gov RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 259-MEDI BP U54 EP U54 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700257 ER PT J AU Petrache, HI AF Petrache, HI TI X-ray and NMR measurements of sterol effects on material properties of lipid bilayers. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIH, Lab Phys & Struct Biol, Bethesda, MD 20892 USA. EM horia@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 087-COLL BP U816 EP U817 PN 1 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602709 ER PT J AU Planalp, RP Ye, N Park, G Przyborowska, AM Sloan, PE Clifford, T Bauer, CB Broker, GA Rogers, RD Ma, R Torti, SV Brechbiel, MW AF Planalp, RP Ye, N Park, G Przyborowska, AM Sloan, PE Clifford, T Bauer, CB Broker, GA Rogers, RD Ma, R Torti, SV Brechbiel, MW TI Nickel(II), copper(II) and zinc(II) binding properties and cytotoxicity of tripodal, hexadentate tris(ethylenediamine)-analogue chelators. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Univ New Hampshire, Dept Chem, Durham, NH 03824 USA. NIH, Radiat Oncol Branch, Bethesda, MD 20892 USA. Univ Alabama, Dept Chem, Tuscaloosa, AL 35487 USA. Univ Alabama, Ctr Green Mdg, Tuscaloosa, AL 35487 USA. EM roy.planalp@unh.edu RI Rogers, Robin/C-8265-2013 OI Rogers, Robin/0000-0001-9843-7494 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 107-INOR BP U1303 EP U1303 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655604465 ER PT J AU Sackett, DL Boukari, H Watts, N Nossal, R AF Sackett, DL Boukari, H Watts, N Nossal, R TI Nanoscopic protein ring polymers composed of tubulin and induced by small peptides and depsipeptides. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Natl Inst Child Hlth & Human Dev, Lab Integrat & Med Biophys, NIH, Bethesda, MD 20892 USA. EM Dlsackett@aol.com NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 038-COLL BP U809 EP U809 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655602660 ER PT J AU Shi, ZD Lee, K Liu, HP Zhang, MC Roberts, LR Fisher, RJ Yang, DJ Bottaro, D Linehan, M Burke, TR AF Shi, ZD Lee, K Liu, HP Zhang, MC Roberts, LR Fisher, RJ Yang, DJ Bottaro, D Linehan, M Burke, TR TI Design, synthesis and utilization of biotinylated macrocyclic Grb2 SH2 domain-binding ligands. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Med Chem Lab, CCR, NIH, Frederick, MD 21702 USA. Univ Michigan, Sch Med, Dept Hematol Oncol, Ann Arbor, MI 48109 USA. SAIC Frederick, Prot Chem Lab, Frederick, MD USA. NCI, Urol Oncol Branch, CCR, NIH, Bethesda, MD 20892 USA. EM shiz@ncifcrf.gov RI Fisher, Robert/B-1431-2009; Bottaro, Donald/F-8550-2010 OI Bottaro, Donald/0000-0002-5057-5334 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 040-MEDI BP U12 EP U12 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700040 ER PT J AU Showalter, BM Saavedra, JE Citron, ML Keefer, LK AF Showalter, BM Saavedra, JE Citron, ML Keefer, LK TI Potential prodrugs of nitric oxide-releasing PROLI/NO. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Chem Sect, Comparat Carcinogenesis Lab, Frederick, MD 21702 USA. NCI, BRP, SAIC Frederick Inc, Frederick, MD 21701 USA. EM bshowalter@ncifcrf.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 237-MEDI BP U49 EP U49 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700235 ER PT J AU Svarovsky, S Barchi, JJ AF Svarovsky, S Barchi, JJ TI Development of multivalient gold and semiconductor nanoparticles encapsulated with tumor associated glycoantigens as antimetastatic and early cancer detection agents. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Med Chem Lab, Frederick, MD 21702 USA. EM ssvarovs@ncifcrf.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 070-CARB BP U274 EP U274 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655601044 ER PT J AU Tchilibon, S Kim, SA Gao, ZG Harris, BA Blaustein, J Gross, AS Duong, HT Melman, N Jacobson, K AF Tchilibon, S Kim, SA Gao, ZG Harris, BA Blaustein, J Gross, AS Duong, HT Melman, N Jacobson, K TI Exploring distal regions of the A(3) adenosine receptor binding site: Sterically-constrained N-6-(2-phenylethyl)-adenosine derivatives as potent ligands. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. EM susannat@intra.niddk.nih.gov RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 257-MEDI BP U54 EP U54 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700255 ER PT J AU Waterhouse, DJ Saavedra, JE Citro, ML Buzard, GS Ji, XH Xu, X Fox, SD Veenstra, TD Keefer, LK AF Waterhouse, DJ Saavedra, JE Citro, ML Buzard, GS Ji, XH Xu, X Fox, SD Veenstra, TD Keefer, LK TI Analytical challenges faced in designing potential anticancer agents from nitric oxide (NO)-releasing diazeniumdiolate prodrugs. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NCI, Comparat Carcinogenesis Lab, Chem Sect, Ft Detrick, MD 21702 USA. SAIC Frederick Inc, NCI Frederick, BRP, Frederick, MD USA. NCI Frederick, Biomol Struct Sect, Macromol Crystallog Lab, Ft Detrick, MD 21702 USA. SAIC Frederick, Lab Proteom & Analyt Technol, Frederick, MD USA. EM waterhoused@ncifcrf.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 081-ANYL BP U91 EP U91 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655600369 ER PT J AU Wess, J Li, B Hamdan, FF Brichta, L Nowak, N Bloodworth, L Schmidt, C Han, SJ AF Wess, J Li, B Hamdan, FF Brichta, L Nowak, N Bloodworth, L Schmidt, C Han, SJ TI Structure-function analysis of the M3 muscarinic acetylcholine receptor using disulfide cross-linking and receptor random mutagenesis approaches. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NIDDK, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. EM jwess@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 206-MODI BP U43 EP U44 PN 2 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700204 ER PT J AU Wu, XW Brooks, BR AF Wu, XW Brooks, BR TI Spherical periodical transform of long-range interactions for molecular simulation. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. EM WuXW@nhlbi.nih.gov; brbrooks@helix.nih.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 302-COMP BP U1031 EP U1031 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655603469 ER PT J AU Xiao, XS Antony, S Kohlhagen, G Pommier, Y Cushman, M AF Xiao, XS Antony, S Kohlhagen, G Pommier, Y Cushman, M TI Design, synthesis, and biological evaluation of novel cytotoxic aminoalkenylindenoisoquinoline topoisomerase I inhibitors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th ACS National Meeting CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA. NCI, Mol Pharmacol Lab, Bethesda, MD 20892 USA. EM xsxiao@pharmacy.purdue.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 113-MEDI BP U26 EP U26 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AK UT WOS:000223655700112 ER PT J AU Zhu, JW Testerman, R Luo, J Ekstrom, D Spenser, G Miller, D Ward, L Reeb, S Jiang, H Burnette, A Mitra, G AF Zhu, JW Testerman, R Luo, J Ekstrom, D Spenser, G Miller, D Ward, L Reeb, S Jiang, H Burnette, A Mitra, G TI Fermentation process development of F5 anti-Erb B2 single chain antibody expressed in E. coli. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 227th National Meeting of the American-Chemical Society CY MAR 28-APR 01, 2004 CL Anaheim, CA SP Amer Chem Soc C1 SAIC Frederick, Biopharmaceut Dev Program, NCI, Ft Detrick, MD 21702 USA. SAIC Frederick, Prot Express Lab, NCI, Ft Detrick, MD 21702 USA. EM jianweiz@ncifcrf.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 28 PY 2004 VL 227 MA 073-BIOT BP U134 EP U134 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 851AJ UT WOS:000223655600619 ER PT J AU Wallace, GL Treffert, DA AF Wallace, GL Treffert, DA TI Head size and autism SO LANCET LA English DT Editorial Material ID CIRCUMFERENCE; DISORDER; CHILDREN; GROWTH; LIFE; AGE C1 NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. St Agnes Hosp, Fond Du Lac, WI USA. Univ Wisconsin, Sch Med, Dept Psychiat, Madison, WI USA. RP Wallace, GL (reprint author), NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. EM gregwallace@mail.nih.gov RI Wallace, Gregory/A-4789-2008; OI Wallace, Gregory/0000-0003-0329-5054 NR 14 TC 4 Z9 4 U1 0 U2 2 PU LANCET LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0140-6736 J9 LANCET JI Lancet PD MAR 27 PY 2004 VL 363 IS 9414 BP 1003 EP 1004 DI 10.1016/S0140-6736(04)15877-7 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 807JH UT WOS:000220497300003 PM 15051277 ER PT J AU Kirk, AD AF Kirk, AD TI Ethics in the quest for transplant tolerance SO TRANSPLANTATION LA English DT Article ID RENAL-ALLOGRAFT RECIPIENTS; UNITED-STATES; INDUCTION; KIDNEY; CYCLOSPORINE; MONOTHERAPY; CAMPATH-1H; ANTIBODY AB This article will examine internationally accepted guidelines for human experimentation as they relate to modern organ transplantation trials. Specific reference to recent clinical trials and the growing diversity of immunosuppressive protocols will be highlighted. C1 NIDDKD, Transplantat Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RP Kirk, AD (reprint author), NIDDKD, Transplantat Branch, NIH, Dept Hlth & Human Serv, Bethesda, MD 20892 USA. RI Kirk, Allan/B-6905-2012 NR 28 TC 10 Z9 10 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD MAR 27 PY 2004 VL 77 IS 6 BP 947 EP 951 DI 10.1097/01.TP.0000117778.83216.29 PG 5 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA 806VD UT WOS:000220460500031 PM 15077045 ER PT J AU Gloster, SE Newton, P Cornforth, D Lifson, JD Williams, I Shaw, GM Borrow, P AF Gloster, SE Newton, P Cornforth, D Lifson, JD Williams, I Shaw, GM Borrow, P TI Association of strong virus-specific CD4 T cell responses with efficient natural control of primary HIV-1 infection SO AIDS LA English DT Article DE HIV-1; CD4; cellular immunity; T cell help; acute infection ID HUMAN-IMMUNODEFICIENCY-VIRUS; DENDRITIC CELLS; VIRAL-INFECTION; IMMUNE FUNCTION; BONE-MARROW; IN-VIVO; PLASMA; LYMPHOCYTE; VIREMIA; CD8(+) AB Objective: To investigate whether there are differences in the virus-specific CD4 T cell response during primary HIV-1 infection in patients who naturally (without antiretroviral intervention) control viral replication with differing efficiencies. Methods: CD4 T cell responses to recombinant HIV proteins (Gag p24 and p55 and Env gp160) and an inactivated HIV-1 preparation were analysed using interferon-gamma ELISPOT assays (with CD8-depleted peripheral blood mononuclear cells) and by intracellular interferon-gamma staining and fluorescent-activated cell sorting. Results: Strong HIV-specific CD4 T cell responses were detected from the earliest time-points analysed in primary infection in patients who naturally established low persisting viral loads. By contrast, HIV-specific CD4 T cell responses were weaker (at or just below the limit of detection in our assays) at similar time-points in patients who went on to establish high persisting viral loads. Statistical analysis revealed a highly significant difference (P < 0.001) between the magnitudes of the Gag p24-specific response at the earliest time-point analysed in primary infection in the two sets of patients. Conclusions: Strong HIV-specific CD4 T cell responses are associated with efficient natural control of primary HIV-1 infection. (C) 2004 Lippincott Williams Wilkins. C1 Edward Jenner Inst Vaccine Res, Newbury RG20 7NN, Berks, England. UCL Royal Free & Univ Coll, Sch Med, Ctr Sexual Hlth & HIV Res, London, England. Camden Primary Care Trust, London, England. Natl Canc Inst, AIDS Vaccine Program, SAIC Frederick Inc, Frederick, MD USA. Univ Alabama, Howard Hughes Med Inst, Div Hematol Oncol, Dept Med, Birmingham, AL 35294 USA. RP Borrow, P (reprint author), Edward Jenner Inst Vaccine Res, Newbury RG20 7NN, Berks, England. FU NIAID NIH HHS [AI35467, AI37430, AI41530]; PHS HHS [N01-C0-124000] NR 39 TC 41 Z9 43 U1 1 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0269-9370 J9 AIDS JI Aids PD MAR 26 PY 2004 VL 18 IS 5 BP 749 EP 755 DI 10.1097/01.aids.0000111401.02002.92 PG 7 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA 816DO UT WOS:000221090800005 PM 15075509 ER PT J AU Shieh, JJ Pan, CJ Mansfield, BC Chou, JY AF Shieh, JJ Pan, CJ Mansfield, BC Chou, JY TI The islet-specific glucose-6-phosphatase-related protein, implicated in diabetes, is a glycoprotein embedded in the endoplasmic reticulum membrane SO FEBS LETTERS LA English DT Article DE glucose-6-phosphatase; diabetes mellitus; membrane topography; endoplasmic reticuluin; proteasome; transmembrane domain ID STORAGE-DISEASE TYPE-1A; SUBUNIT-RELATED PROTEIN; ASPARAGINE-LINKED OLIGOSACCHARIDES; GENE; IDENTIFICATION; RAT; DEGRADATION; LACTACYSTIN; CLONING; LIVER AB The islet-specific glucose-6-phosphatase-related protein (IGRP) has no known catalytic activity, but is of interest because it is the source of the peptide autoantigen targeted by a prevalent population of pathogenic CD8(+) T cells in non-obese diabetic mice. To better understand the potential roles of this protein in diabetes mellitus, we examine the subcellular localization and membrane topography of human IGRP. We show that IGRP is a glycoprotein, held in the endoplasmic reticulum by nine transmembrane domains, which is degraded in cells predominantly through the proteasome pathway that generates the major histocompatibility complex class I-presented peptides. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. C1 NICHHD, Sect Cellular Differectiat, Heritable Disorders Branch, NIH, Bethesda, MD 20892 USA. RP Chou, JY (reprint author), NICHHD, Sect Cellular Differectiat, Heritable Disorders Branch, NIH, Bldg 10,Room 9S241, Bethesda, MD 20892 USA. EM chouja@mail.nih.gov OI Mansfield, Brian/0000-0002-8533-2789 NR 33 TC 18 Z9 20 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 EI 1873-3468 J9 FEBS LETT JI FEBS Lett. PD MAR 26 PY 2004 VL 562 IS 1-3 BP 160 EP 164 DI 10.1016/S0014-5793(04)00223-6 PG 5 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 808ES UT WOS:000220553000027 PM 15044018 ER PT J AU Jones, CE Mueser, TC Nossal, NG AF Jones, CE Mueser, TC Nossal, NG TI Bacteriophage T4 32 protein is required for helicase-dependent leading strand synthesis when the helicase is loaded by the T4 59 helicase-loading protein SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID GENE 32 PROTEIN; DNA-BINDING-PROTEIN; POLAR BRANCH MIGRATION; REPLICATION FORK; ASSEMBLY PROTEIN; NUCLEIC-ACIDS; MACROMOLECULAR MACHINES; MINICIRCLE SUBSTRATE; CRYSTAL-STRUCTURE; POLYMERASE GP43 AB In the bacteriophage T4 DNA replication system, T4 gene 59 protein binds preferentially to fork DNA and accelerates the loading of the T4 41 helicase. 59 protein also binds the T4 32 single-stranded DNA-binding protein that coats the lagging strand template. Here we explore the function of the strong affinity between the 32 and 59 proteins at the replication fork. We show that, in contrast to the 59 helicase loader, 32 protein does not bind forked DNA more tightly than linear DNA. 32 protein displays a strong binding polarity on fork DNA, binding with much higher affinity to the 5' single-stranded lagging strand template arm of a model fork, than to the 3' single-stranded leading strand arm. 59 protein promotes the binding of 32 protein on forks too short for cooperative binding by 32 protein. We show that 32 protein is required for helicase-dependent leading strand DNA synthesis when the helicase is loaded by 59 protein. However, 32 protein is not required for leading strand synthesis when helicase is loaded, less efficiently, without 59 protein. Leading strand synthesis by wild type T4 polymerase is strongly inhibited when 59 protein is present without 32 protein. Because 59 protein can load the helicase on forks without 32 protein, our results are best explained by a model in which 59 helicase loader at the fork prevents the coupling of the leading strand polymerase and the helicase, unless the position of 59 protein is shifted by its association with 32 protein. C1 NIDDK, Mol & Cellular Biol Lab, NIH, Bethesda, MD 20892 USA. Univ Toledo, Dept Chem, Toledo, OH 43606 USA. RP Nossal, NG (reprint author), NIDDK, Mol & Cellular Biol Lab, NIH, Bldg 8,Rm 2A19, Bethesda, MD 20892 USA. EM ngn@helix.nih.gov NR 54 TC 23 Z9 23 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12067 EP 12075 DI 10.1074/jbc.M313840200 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900010 PM 14729909 ER PT J AU Ogushi, K Wada, A Niidome, T Okuda, T Llanes, R Nakayama, M Nishi, Y Kurazono, H Smith, KD Aderem, A Moss, J Hirayama, T AF Ogushi, K Wada, A Niidome, T Okuda, T Llanes, R Nakayama, M Nishi, Y Kurazono, H Smith, KD Aderem, A Moss, J Hirayama, T TI Gangliosides act as co-receptors for Salmonella enteritidis FliC and promote FliC induction of human beta-defensin-2 expression in Caco-2 cells SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PROTEIN-KINASE PATHWAYS; TOLL-LIKE RECEPTORS; EPITHELIAL-CELLS; BETA-DEFENSINS; HELICOBACTER-PYLORI; BACTERIAL FLAGELLIN; DENDRITIC CELLS; INNATE IMMUNITY; INFLUENZA-VIRUS; LINKING INNATE AB Antimicrobial peptides such as defensins are crucial for host defense at mucosal surfaces. We reported previously that Salmonella enteritidis flagellin (FliC) induced human beta-defensin-2 (hBD-2) mRNA expression in Caco-2 cells via NF-kappaB activation (Ogushi, K., Wada, A., Niidome, T., Mori, N., Oishi, K., Nagatake, T., Takahashi, A., Asakura, H., Makino, S., Hojo, H., Nakahara, Y., Ohsaki, M., Hatakeyama, T., Aoyagi, H., Kurazono, H., Moss, J., and Hirayama, T. (2001) J. Biol. Chem. 276, 30521-30526). In this study, we examined the role of ganglioside as co-receptors with Toll-like receptor 5 (TLR5) on FliC induction of hBD-2 expression in Caco-2 cells. Exogenous gangliosides suppressed FliC induction of hBD-2 promoter activity and binding of FliC to Caco-2 cells. Incorporation of exogenous ganglioside GD1a into Caco-2 cell membranes increased the effect of FliC on hBD-2 promoter activity. In support of a role for endogenous gangliosides, incubation of Caco-2 cells with DL-threo-2-hexadecanoylamino-3-morpholino-1-phenylpropanol, a glucosylceramide synthase inhibitor, reduced FliC induction of hBD-2 promoter activity. GD1a-loaded CHO-K1-expressing TLR5 cells had a higher potential for hBD-2 induction following FliC stimulation than GD1a-loaded CHO-K1 cells not expressing TLR5. FliC increased phosphorylation of mitogen-activated protein kinase, p38, and ERK1/2. Exogenous gangliosides GD1a, GD1b, and GT1b each suppressed FliC induction of p38 and ERK1/2 phosphorylation. Furthermore, FliC did not enhance luciferase activity in Caco-2 cells transfected with a plasmid containing a mutated activator protein 1-binding site. These results suggest that gangliosides act as co-receptors with TLR5 for FliC and promote hBD-2 expression via mitogen-activated protein kinase. C1 Nagasaki Univ, Inst Trop Med, Dept Bacteriol, Nagasaki 8528523, Japan. Nagasaki Univ, Fac Engn, Dept Appl Chem, Nagasaki 8528521, Japan. Okayama Univ, Sch Med, Fac Hlth Sci, Okayama 7008558, Japan. Univ Washington, Dept Pathol, Seattle, WA 98195 USA. Inst Syst Biol, Seattle, WA 98103 USA. NHLBI, Pulm Crit Care Med Branch, NIH, Bethesda, MD 20892 USA. RP Hirayama, T (reprint author), Nagasaki Univ, Inst Trop Med, Dept Bacteriol, Nagasaki 8528523, Japan. EM hirayama@net.nagasaki-u.ac.jp RI Niidome, Takuro/F-1508-2010 OI Niidome, Takuro/0000-0002-8070-8708 NR 51 TC 36 Z9 39 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12213 EP 12219 DI 10.1074/jbc.M307944200 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900027 PM 14707135 ER PT J AU Dohke, Y Oh, YS Ambudkar, IS Turner, RJ AF Dohke, Y Oh, YS Ambudkar, IS Turner, RJ TI Biogenesis and topology of the transient receptor potential Ca2+ channel TRPC1 SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID IN-VITRO TRANSLATION; ENDOPLASMIC-RETICULUM MEMBRANE; TRANSMEMBRANE SEGMENTS; ANION-EXCHANGER; PROTEIN; PREDICTION; INSERTION; IDENTIFICATION; TRANSLOCATION; INTEGRATION AB The TRPC ion channels are candidates for the store-operated Ca2+ entry pathway activated in response to depletion of intracellular Ca2+ stores. Hydropathy analyses indicate that these proteins contain eight hydrophobic regions (HRs) that could potentially form alpha-helical membrane-spanning segments. Based on limited sequence similarities to other ion channels, it has been proposed that only six of the eight HRs actually span the membrane and that the last two membrane-spanning segments (HRs 6 and 8) border the ion-conducting pore of which HR 7 forms a part. Here we study the biogenesis and transmembrane topology of human TRPC1 to test this model. We have employed a truncation mutant approach combined with insertions of glycosylation sites into full-length TRPC1. In our truncation mutants, portions of the TRPC1 sequence containing one or more HRs were fused between the enhanced green fluorescent protein and a C-terminal glycosylation tag. These chimeras were transiently expressed in the human embryonic cell line HEK-293T. Glycosylation of the tag was used to monitor its location relative to the lumen of the endoplasmic reticulum and thereby HR orientation. Our data indicate that HRs 1, 4, and 6 cross the membrane from cytosol to the ER lumen, that HRs 2, 5, and 8 have the opposite orientation, and that HR 3 is left out of the membrane on the cytosolic side. Our results also show that the sequence downstream of HR 8 plays an important role in anchoring its C-terminal end on the cytosolic side of the membrane. This effect appears to prevent HR 7 from spanning the bilayer and to result in its forming a pore-like structure of the type previously envisioned for the TRPC channels. We speculate that a similar mechanism may be responsible for the formation of other ion channel pores. C1 Natl Inst Dent & Craniofacial Res, Membrane Biol Sect, NIH, DHHS, Bethesda, MD 20892 USA. Natl Inst Dent & Craniofacial Res, Secretory Physiol Sec, Gene Therepy & Therapeut Branch, NIH,DHHS, Bethesda, MD 20892 USA. RP Turner, RJ (reprint author), NIH, Bldg 10,Rm 1A01,10 Ctr Dr,MSC 1190, Bethesda, MD 20892 USA. EM rjturner@nih.gov NR 44 TC 26 Z9 30 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12242 EP 12248 DI 10.1074/jbc.M312456200 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900030 PM 14707123 ER PT J AU Akari, H Fujita, M Kao, S Khan, MA Shehu-Xhilaga, M Adachi, A Strebel, K AF Akari, H Fujita, M Kao, S Khan, MA Shehu-Xhilaga, M Adachi, A Strebel, K TI High level expression of human immunodeficiency virus type-1 Vif inhibits viral infectivity by modulating proteolytic processing of the gag precursor at the p2/nucleocapsid processing site SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HIV-1 VIF; ANTIVIRAL ACTIVITY; CO-ENCAPSIDATION; ENZYME APOBEC3G; PROTEIN BINDS; CELL-LINE; DNA; DEGRADATION; VIRIONS; STABILITY AB The human immunodeficiency virus type-1 Vif protein has a crucial role in regulating viral infectivity. However, we found that newly synthesized Vif is rapidly degraded by cellular proteases. We tested the dose dependence of Vif in non-permissive H9 cells and found that Vif, when expressed at low levels, increased virus infectivity in a dose-dependent manner. Surprisingly, however, the range of Vif required for optimal virus infectivity was narrow, and further increases in Vif severely reduced viral infectivity. Inhibition of viral infectivity at higher levels of Vif was cell type-independent and was associated with an accumulation of Gag-processing intermediates. Vif did not act as a general protease inhibitor but selectively inhibited Gag processing at the capsid and nucleocapsid (NC) boundary. Identification of Vif variants that were efficiently packaged but were unable to modulate Gag processing suggests that Vif packaging was necessary but insufficient for the production of 33- and 34-kDa processing intermediates. Interestingly, these processing intermediates, like Vif, associated with viral nucleoprotein complexes more rigidly than mature capsid and NC. We conclude that virus-associated Vif inhibits processing of a subset of Gag precursor molecules at the p2/NC primary cleavage site. Modulation of processing of a small subset of Gag molecules by physiological levels of Vif may be important for virus maturation. However, the accumulation of such processing intermediates at high levels of Vif is inhibitory. Thus, rapid intracellular degradation of Vif may have evolved as a mechanism to prevent such inhibitory effects of Vif. C1 NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA. Natl Inst Infect Dis, Tsukuba Primate Ctr Med Sci, Ibaraki 3050843, Japan. Univ Tokushima, Grad Sch Med, Dept Virol, Tokushima 7708502, Japan. RP Strebel, K (reprint author), NIAID, Mol Microbiol Lab, NIH, 4-312,4 Ctr Dr,MSC 0460, Bethesda, MD 20892 USA. EM kstrebel@nih.gov RI botla, Gomathi/A-5724-2008 NR 46 TC 44 Z9 45 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12355 EP 12362 DI 10.1074/jbc.M312426200 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900043 PM 14722068 ER PT J AU Gamero, AM Sakamoto, S Montenegro, J Larner, AC AF Gamero, AM Sakamoto, S Montenegro, J Larner, AC TI Identification of a novel conserved motif in the STAT family that is required for tyrosine phosphorylation SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ALPHA-INTERFERON; GENE-EXPRESSION; V-ABL; ACTIVATION; PROTEINS; PATHWAY; MUTATIONS; CELLS; DNA; PROLIFERATION AB The rapid transcriptional activation of cellular genes by either type 1 interferons (IFNalpha/beta) or type 2 interferon (IFNgamma) is responsible for many of the pleiotropic effects of these cytokines, including their antiviral, antigrowth, and immunomodulatory activities. Interferon-stimulated gene expression is mediated by transcription factors termed Stats, which upon being tyrosine-phosphorylated, translocate to the nucleus and bind enhancers of interferon-activated genes. We have recently characterized a new Jurkat cell variant, named H123, where IFNalpha stimulates programmed cell death. H123 clones that are resistant to the apoptotic actions of IFNalpha have been selected. One of these clones (Clone 8) is defective in its responses to IFNalpha with regard to activation of genes that require tyrosine phosphorylation of Stat2. Stimulation of Clone 8 cells with IFNalpha induces normal tyrosine phosphorylation of Stat1 and Stat3. Sequencing of Stat2 RNA reveals a substitution of proline 630 located within the Src homology 2 domain of Stat2 to leucine (P630L). Pro-630 and its adjacent amino acids are conserved in all Stat family members but are absent in other proteins that contain Src homology 2 domains. Expression of Stat2 P630L in cells inhibits IFNalpha-stimulated gene expression. These results not only define a critical motif in Stat2 required for its transcriptional activity, but they also provide evidence that resistance to type one IFNs can be mediated by mutations in Stat2 as well as those previously described for Stat1. C1 Cleveland Clin Fdn, Dept Immunol, Lerner Res Inst, Cleveland, OH 44195 USA. NCI, Expt Immunol Lab, NIH, Frederick, MD 21702 USA. RP Larner, AC (reprint author), Cleveland Clin Fdn, Dept Immunol, Lerner Res Inst, NB-3-30,9500 Euclid Ave, Cleveland, OH 44195 USA. EM larnera@ccf.org FU NCI NIH HHS [CA 77366] NR 23 TC 11 Z9 12 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12379 EP 12385 DI 10.1074/jbc.M310787200 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900046 PM 14722125 ER PT J AU Ghosh, A Shieh, JJ Pan, CJ Chou, JY AF Ghosh, A Shieh, JJ Pan, CJ Chou, JY TI Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-beta forming a phosphohistidine enzyme intermediate during catalysis SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID STORAGE-DISEASE TYPE-1A; SUBUNIT-RELATED PROTEIN; ACTIVE-SITE; MICROSOMAL GLUCOSE-6-PHOSPHATASE; MURINE GLUCOSE-6-PHOSPHATASE; TRANSMEMBRANE TOPOLOGY; GLUCOSE 6-PHOSPHATASE; CURVULARIA-INAEQUALIS; IDENTIFICATION; GENE AB The glucose-6-phosphatase (Glc-6-Pase) family comprises two active endoplasmic reticulum (ER)-associated isozymes: the liver/kidney/intestine Glc-6-Pase-alpha and the ubiquitous Glc-6-Pase-beta. Both share similar kinetic properties. Sequence alignments predict the two proteins are structurally similar. During glucose 6-phosphate (Glc-6-P) hydrolysis, Glc-6-Pase-beta, a nine-transmembrane domain protein, forms a covalently bound phosphoryl enzyme intermediate through His(176), which lies on the lumenal side of the ER membrane. We showed that Glc-6-Pase-beta is also a nine-transmembrane domain protein that forms a covalently bound phosphoryl enzyme intermediate during Glc-6-P hydrolysis. However, the intermediate was not detectable in Glc-6-Pase-alpha active site mutants R79A, H114A, and H167A. Using [P-32] Glc-6-P coupled with cyanogen bromide mapping, we demonstrated that the phosphate acceptor in Glc-6-Pase-beta is His(167) and that it lies inside the ER lumen with the active site residues, Arg(79) and His(114). Therefore Glc-6-Pase-alpha and Glc-6-Pase-beta share a similar active site structure, topology, and mechanism of action. C1 NICHD, Sect Cellular Differentat, Heritable Disorders Branch, NIH, Bethesda, MD 20892 USA. RP Chou, JY (reprint author), NICHD, Sect Cellular Differentat, Heritable Disorders Branch, NIH, Bldg 10,Rm 9S241, Bethesda, MD 20892 USA. EM chouja@mail.nih.gov NR 32 TC 34 Z9 35 U1 1 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12479 EP 12483 DI 10.1074/jbc.M313271200 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900058 PM 14718531 ER PT J AU Hamasaki-Katagiri, N Molchanova, T Takeda, K Ames, JB AF Hamasaki-Katagiri, N Molchanova, T Takeda, K Ames, JB TI Fission yeast homolog of neuronal calcium sensor-1 (Ncs1p) regulates sporulation and confers calcium tolerance SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SPINDLE POLE BODY; EF-HAND PROTEINS; SCHIZOSACCHAROMYCES-POMBE; SACCHAROMYCES-CEREVISIAE; RHODOPSIN PHOSPHORYLATION; MYRISTOYLATED RECOVERIN; RECOMBINANT RECOVERIN; CA2+-BINDING PROTEINS; CRYSTAL-STRUCTURE; BINDING PROTEIN AB The neuronal calcium sensor (NCS) proteins (e.g. recoverin, neurocalcins, and frequenin) are expressed at highest levels in excitable cells, and some of them regulate desensitization of G protein-coupled receptors. Here we present NMR analysis and genetic functional studies of an NCS homolog in fission yeast (Ncs1p). Ncs1p binds three Ca2+ ions at saturation with an apparent affinity of 2 muM and Hill coefficient of 1.9. Analysis of NMR and fluorescence spectra of Ncs1p revealed significant Ca2+-induced protein conformational changes indicative of a Ca2+-myristoyl switch. The amino-terminal myristoyl group is sequestered inside a hydrophobic cavity of the Ca2+-free protein and becomes solvent-exposed in the Ca2+-bound protein. Subcellular fractionation experiments showed that myristoylation and Ca2+ binding by Ncs1p are essential for its translocation from cytoplasm to membranes. The ncs1 deletion mutant (ncs1Delta) showed two distinct phenotypes: nutrition-insensitive sexual development and a growth defect at high levels of extracellular Ca2+ (0.1 M CaCl2). Analysis of Ncs1p mutants lacking myristoylation (Ncs1p(G2A)) or deficient in Ca2+ binding (Ncs1p(E84Q/E120Q/E168Q)) revealed that Ca2+ binding was essential for both phenotypes, while myristoylation was less critical. Exogenous cAMP, a key regulator for sexual development, suppressed conjugation and sporulation of ncs1Delta, suggesting involvement of Ncs1p in the adenylate cyclase pathway turned on by the glucose-sensing G protein-coupled receptor Git3p. Starvation-independent sexual development of ncs1Delta was also complemented by retinal recoverin, which controls Ca2+-regulated desensitization of rhodopsin. In contrast, the Ca2+ intolerance of ncs1Delta was not affected by cAMP or recoverin, suggesting that the two ncs1Delta phenotypes are mechanistically independent. We propose that Schizosaccharomyces pombe Ncs1p negatively regulates sporulation perhaps by controlling Ca2+-dependent desensitization of Git3p. C1 Univ Maryland, Inst Biotechnol, Ctr Adv Res Biotechnol, Rockville, MD 20850 USA. NHLBI, Pathol Core, NIH, Bethesda, MD 20892 USA. RP Univ Maryland, Inst Biotechnol, Ctr Adv Res Biotechnol, 9600 Gudelsky Dr, Rockville, MD 20850 USA. EM james@carb.nist.gov FU NEI NIH HHS [EY 12347] NR 71 TC 22 Z9 24 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 12744 EP 12754 DI 10.1074/jbc.M311895200 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900088 PM 14722091 ER PT J AU Guo, Q Detweiler, CD Mason, RP AF Guo, Q Detweiler, CD Mason, RP TI Protein radical formation during lactoperoxidase-mediated oxidation of the suicide substrate glutathione - Immunochemical detection of a lactoperoxidase radical-derived 5,5-dimethyl-1-pyrroline N-oxide nitrone adduct SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PROSTAGLANDIN-H SYNTHASE; IRREVERSIBLE ENZYME INACTIVATION; RHO-SIGMA CORRELATION; CYTOCHROME-C-OXIDASE; HORSERADISH-PEROXIDASE; HYDROGEN-PEROXIDE; THYROID PEROXIDASE; THIYL RADICALS; COMPOUND-II; ONE-ELECTRON AB A novel anti-5,5-dimethyl-1-pyrroline N-oxide ( DMPO) polyclonal antiserum that specifically recognizes protein radical-derived DMPO nitrone adducts has been developed. In this study, we employed this new approach, which combines the specificity of spin trapping and the sensitivity of antigen-antibody interactions, to investigate protein radical formation from lactoperoxidase (LPO). When LPO reacted with GSH in the presence of DMPO, we detected an LPO radical-derived DMPO nitrone adduct using enzyme-linked immunosorbent assay and Western blotting. The formation of this nitrone adduct depended on the concentrations of GSH, LPO, and DMPO as well as pH values, and GSH could not be replaced by H2O2. The level of this nitrone adduct was decreased significantly by azide, catalase, ascorbate, iodide, thiocyanate, phenol, or nitrite. However, its formation was unaffected by chemical modification of free cysteine, tyrosine, and tryptophan residues on LPO. ESR spectra showed that a glutathiyl radical was formed from the LPO/GSH/DMPO system, but no protein radical adduct could be detected by ESR. Its formation was decreased by azide, catalase, ascorbate, iodide, or thiocyanate, whereas phenol or nitrite increased it. GSH caused marked changes in the spectrum of compound II of LPO, indicating that GSH binds to the heme of compound II, whereas phenol or nitrite prevented these changes and reduced compound II back to the native enzyme. GSH also dose-dependently inhibited the peroxidase activity of LPO as determined by measuring 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) oxidation. Taken together, these results demonstrate that the GSH-dependent LPO radical formation is mediated by the glutathiyl radical, possibly via the reaction of the glutathiyl radical with the heme of compound II to form a heme-centered radical trapped by DMPO. C1 NIEHS, Lab Pharmacol & Chem, NIH, Res Triangle Pk, NC 27709 USA. RP Guo, Q (reprint author), NIEHS, Lab Pharmacol & Chem, NIH, POB 12233, Res Triangle Pk, NC 27709 USA. EM guo1@niehs.nih.gov NR 85 TC 22 Z9 23 U1 1 U2 9 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 2004 VL 279 IS 13 BP 13272 EP 13283 DI 10.1074/jbc.M310034200 PG 12 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804YV UT WOS:000220334900150 PM 14724284 ER PT J AU Ghosh, T Peterson, B Tomasevic, N Peculis, BA AF Ghosh, T Peterson, B Tomasevic, N Peculis, BA TI Xenopus u8 snoRNA binding protein is a conserved nuclear decapping enzyme SO MOLECULAR CELL LA English DT Article ID SMALL NUCLEOLAR RNAS; SM-LIKE PROTEINS; MESSENGER-RNA; SACCHAROMYCES-CEREVISIAE; CYTOPLASMIC DOMAIN; ESCHERICHIA-COLI; MUTT ENZYME; FAMILY; SEQUENCE; SNRNA AB U8 snoRNP is required for accumulation of mature 5.8S and 28S rRNA in vertebrates. We are identifying proteins that bind U8 RNA with high specificity to understand how U8 functions in ribosome biogenesis. Here, we characterize a Xenopus 29 kDa protein (X29), which we previously showed binds U8 RNA with high affinity. X29 and putative homologs in other vertebrates contain a NUDIX domain found in MutT and other nucleoticle diphosphatases. Recombinant X29 protein has diphosphatase activity that removes m(7)G and m(227)G caps from U8 and other RNAs in vitro; the putative 29 kDa human homolog also displays this decapping activity. X29 is primarily nucleolar in Xenopus tissue culture cells. We propose that X29 is a member of a conserved family of nuclear decapping proteins that function in regulating the level of U8 snoRNA and other nuclear RNAs with methylated caps. C1 NIDDKD, Genet & Biochem Branch, NIH, Bethesda, MD 20892 USA. RP Peculis, BA (reprint author), NIDDKD, Genet & Biochem Branch, NIH, Bldg 8,Room 106, Bethesda, MD 20892 USA. EM bp51h@nih.gov NR 47 TC 45 Z9 47 U1 0 U2 1 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 1097-2765 J9 MOL CELL JI Mol. Cell PD MAR 26 PY 2004 VL 13 IS 6 BP 817 EP 828 DI 10.1016/S1097-2765(04)00127-3 PG 12 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 808PX UT WOS:000220582100009 PM 15053875 ER PT J AU Plech, A Wulff, M Bratos, S Mirloup, F Vuilleumier, R Schotte, F Anfinrud, PA AF Plech, A Wulff, M Bratos, S Mirloup, F Vuilleumier, R Schotte, F Anfinrud, PA TI Visualizing chemical reactions in solution by picosecond x-ray diffraction SO PHYSICAL REVIEW LETTERS LA English DT Article ID MOLECULAR-IODINE; REAL-TIME; GEMINATE RECOMBINATION; ULTRAFAST DIFFRACTION; HYDROGEN-BONDS; DYNAMICS; PHOTODISSOCIATION; CRYSTALLOGRAPHY; SCATTERING; RESOLUTION AB We present a time-resolved x-ray diffraction study to monitor the recombination of laser-dissociated iodine molecules dissolved in CCl4. The change in structure of iodine is followed during the whole recombination process. The deexcitation of solute molecules produces a heating of the solvent and induces tiny changes in its structure. The variations in the distance between pairs of chlorine atoms in adjacent CCl4 molecules are probed on the mangle length scale. However, the most striking outcome of the present work is the experimental determination of temporally varying atom-atom pair distribution functions. Variations of the mean density of the solution during thermal expansion are also followed in real time. One concludes that not only time-resolved optical spectroscopy but also time-resolved x-ray diffraction can be used to monitor atomic motions in liquids. C1 Univ Konstanz, Fachbereich Phys, D-78457 Constance, Germany. European Synchrotron Radiat Facil, F-38043 Grenoble, France. Univ Paris 06, Phys Theor Liquides Lab, F-75252 Paris, France. NIDDK, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Plech, A (reprint author), Univ Konstanz, Fachbereich Phys, Univ Str 10, D-78457 Constance, Germany. RI Plech, Anton/E-4895-2010; OI Plech, Anton/0000-0002-6290-9303 NR 27 TC 92 Z9 93 U1 1 U2 8 PU AMERICAN PHYSICAL SOC PI COLLEGE PK PA ONE PHYSICS ELLIPSE, COLLEGE PK, MD 20740-3844 USA SN 0031-9007 J9 PHYS REV LETT JI Phys. Rev. Lett. PD MAR 26 PY 2004 VL 92 IS 12 AR 125505 DI 10.1103/PhysRevLett.92.125505 PG 4 WC Physics, Multidisciplinary SC Physics GA 807TU UT WOS:000220524600034 PM 15089686 ER PT J AU Xu, LF Kulkarni, SS Izenwasser, S Katz, JL Kopajtic, T Lomenzo, SA Newman, AH Trudell, ML AF Xu, LF Kulkarni, SS Izenwasser, S Katz, JL Kopajtic, T Lomenzo, SA Newman, AH Trudell, ML TI Synthesis and monoamine transporter binding of 2-(diarylmethoxymethyl)-3 beta-aryltropane derivatives SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID DOPAMINE UPTAKE INHIBITION; ACID METHYL-ESTERS; SELECTIVE COMPOUNDS; LIGAND-BINDING; HIGH-AFFINITY; COCAINE RECEPTOR; BIOLOGICAL EVALUATION; AMIDE ANALOGS; SEROTONIN; POTENT AB 3beta-Aryltropane analogues wherein the 2-position was substituted with various diarylmethoxy-alkyl groups were synthesized and evaluated for binding at the dopamine transporter (DAT), serotonin transporter (SERT), norepinephrine transporter (NET), and muscarinic (M-1) receptors. The 2beta-analogues 9a-i generally demonstrated high to moderate binding affinities (K-i = 34112 nM) at the DAT with good selectivity over SERT, NET, and M-1 receptors. Alternatively, the 2alpha-isomers 10a-i were 10-fold less potent at the DAT with poor selectivity over SERT. These SAR studies provide further evidence for the varied binding requirements of structurally diverse tropane-based ligands and support future studies to elucidate DAT binding requirements in relation to cocaine-like behavioral endpoints. C1 Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. Univ Miami, Sch Med, Dept Psychiat & Behav Sci, Miami, FL 33136 USA. NIDA, Medicat Discovery Res Branch, Intramural Res Program, Baltimore, MD 21224 USA. RP Trudell, ML (reprint author), Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. EM mtrudell@uno.edu RI Izenwasser, Sari/G-9193-2012 FU NIDA NIH HHS [DA11528] NR 40 TC 11 Z9 11 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR 25 PY 2004 VL 47 IS 7 BP 1676 EP 1682 DI 10.1021/jm030430a PG 7 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA 804SE UT WOS:000220317600012 PM 15027858 ER PT J AU Pettit, GR Hoffmann, H Herald, DL Blumberg, PM Hamel, E Schmidt, JM Chang, Y Pettit, RK Lewin, NE Pearce, LV AF Pettit, GR Hoffmann, H Herald, DL Blumberg, PM Hamel, E Schmidt, JM Chang, Y Pettit, RK Lewin, NE Pearce, LV TI Antineoplastic agents. 499. Synthesis of hystatin 2 and related 1H-benzo[de][1,6]-naphthyridinium salts from aaptamine SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID PROTEIN-KINASE-C; NATURAL PRODUCT; ALKALOIDS; SPONGE; DERIVATIVES; ANALOGS; ISOAAPTAMINE; METABOLITES; INHIBITORS AB The marine sponge constituent aaptamine (1) has been converted to the cancer cell growth inhibitor and antibiotic designated hystatin 2 (8a). Herein, we also report results of an initial SAR evaluation of new benzyl derivatives of aaptamine (1). Single benzylation was found to occur at nitrogen N-4 and led to the formation of the 4-benzylaaptamine derivatives 7a-c, whereas double benzylation gave the quaternary 1H-benzo[de][1,6]-naphthyridinium salts 8a-c. The anticancer and antimicrobial properties of these aaptamine derivatives are described. The quaternary ammonium salts 8a (hystatin 2) and 8b exhibited significant inhibitory activity against the murine P388 lymphocytic leukemia and a minipanel of human cancer cell lines. Salts 8a and 8b also had broad spectrum antimicrobial activities and were most potent against Mycobacterium tuberculosis, Neisseria gonorrhoeae, and Micrococcus luteus. Naphthyridinium chloride Sa was selected for further development, and results of an initial cell cycle analysis and a cDNA microarray study showed effects consistent with inhibition of the S-phase of cell growth. C1 Arizona State Univ, Inst Canc Res, Tempe, AZ 85287 USA. Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA. Arizona State Univ, Dept Microbiol, Tempe, AZ 85287 USA. Natl Canc Inst, Mol Mech Tumor Promot Sect, LCCTP, Bethesda, MD 20892 USA. NCI, Screening Technol Branch, Dev Therapeut Program, Div Canc Treatment,NIH, Frederick, MD 21702 USA. RP Pettit, GR (reprint author), Arizona State Univ, Inst Canc Res, Box 872404, Tempe, AZ 85287 USA. FU NCI NIH HHS [CA44344-05-12, R01 CA90441-01] NR 36 TC 17 Z9 17 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR 25 PY 2004 VL 47 IS 7 BP 1775 EP 1782 DI 10.1021/jm030070r PG 8 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA 804SE UT WOS:000220317600023 PM 15027869 ER PT J AU Yoshida, S Meyer, OGJ Rosen, TC Haufe, G Ye, S Sloan, MJ Kirk, KL AF Yoshida, S Meyer, OGJ Rosen, TC Haufe, G Ye, S Sloan, MJ Kirk, KL TI Fluorinated phenylcyclopropylamines. 1. Synthesis and effect of fluorine substitution at the cyclopropane ring on inhibition of microbial tyramine oxidase SO JOURNAL OF MEDICINAL CHEMISTRY LA English DT Article ID SENSITIVE AMINE OXIDASE; MONOAMINE-OXIDASE; ACTIVE-SITE; TOPA QUINONE; SELECTIVE INHIBITORS; CATALYTIC MECHANISM; COPPER; 1-PHENYLCYCLOPROPYLAMINE; INACTIVATION; RESOLUTION AB Two series of diastereopure phenylcyclopropylamine analogues, 2-fluoro-2-phenylcyclopropylamines and 2-fluoro-2-phenylcyclopropylalkylamines, as well as 2-fluoro-1-phenylcyclopropylamines and 2-fluoro-1-phenyleyclopropylmethylamines, were synthesized in order to study the effects of fluorine substitution on monoamine oxidase inhibition. Inhibitory activity was assayed using commercially available microbial tyramine oxidase. Characterization of tyramine oxidase, carried out prior to the inhibition experiments, confirmed earlier suggestions that this enzyme is a semicarbazide-sensitive copper-containing monoamine oxidase. The most potent competitive inhibitor was trans-2-fluoro-2-phenylcyclopropylamine, which had an IC50 value 10 times lower than that of the nonfluorinated compound, tranylcypromine. 2-Fluoro-1-phenylcyclopropylmethylamine was found to be a weak noncompetitive inhibitor of tyramine oxidase. The presence of a free amino group, directly bonded to the cyclopropane ring, and a fluorine atom in a relationship cis to the amino group were structural features that increased tyramine oxidase inhibition. C1 NIDDKD, Bioorgan Chem Lab, US Dept HHS, NIH, Bethesda, MD 20892 USA. Univ Munster, Inst Organ Chem, D-48109 Munster, Germany. RP Kirk, KL (reprint author), NIDDKD, Bioorgan Chem Lab, US Dept HHS, NIH, Bethesda, MD 20892 USA. EM kennethk@bdg8.niddk.nih.gov NR 40 TC 29 Z9 29 U1 0 U2 6 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0022-2623 J9 J MED CHEM JI J. Med. Chem. PD MAR 25 PY 2004 VL 47 IS 7 BP 1796 EP 1806 DI 10.1021/jm030398k PG 11 WC Chemistry, Medicinal SC Pharmacology & Pharmacy GA 804SE UT WOS:000220317600026 PM 15027872 ER PT J AU Ivanic, J Collins, JR Burt, SK AF Ivanic, J Collins, JR Burt, SK TI Theoretical study of the low lying electronic states of oxoX(salen) (X = Mn, Mn-, Fe, and Cr-) complexes SO JOURNAL OF PHYSICAL CHEMISTRY A LA English DT Article ID ASYMMETRIC ALKENE EPOXIDATION; MOLECULAR-ORBITAL METHODS; DEGENERATE PERTURBATION-THEORY; JACOBSEN-KATSUKI EPOXIDATION; GAUSSIAN-TYPE BASIS; ORGANIC-MOLECULES; SALEN COMPLEXES; MCSCF THEORY; BASIS-SETS; SUBSTITUENTS AB The lowest lying electronic states of oxoX(salen) (X = Mn, Mn-, Fe and Cr) complexes have been studied using complete active space self-consistent field (CASSCF) calculations. These wave functions have been analyzed, via the use of localized and natural orbitals, to identify the electronic structure contributions to the chemistry of these systems. It is found that the electronic structures of all complexes can be rationalized through the use of a common orbital energy diagram. Single point multireference Moller-Plesset (MRMP2) perturbation theory calculations have been performed for the oxoMn(salen) system and these results verify that the CASSCF method gives accurate energy separations for the electronic states. We find that the CASSCF method predicts bound species for all compounds except for the triplet and quintet states of the oxoMn(salen) complex, which spontaneously dissociate in the gas phase. Calculations on the separated species [(5)A Mn(salen) + (3)p O] indicate that the bound singlet species lies some 30 kcal/mol above the dissociated products at the CASSCF level. Attempts to obtain MRMP2 energies for the supersystem of the separated species failed; hence a more accurate value of the dissociation energy could not be determined. We also find, from CASSCF calculations, that the oxoMn(salen) complex is predicted to be a very potent electrophile. C1 NCI, Adv Biomed Comp Ctr, SAIC Frederick, Ft Detrick, MD 21702 USA. RP Ivanic, J (reprint author), NCI, Adv Biomed Comp Ctr, SAIC Frederick, Contract 1-CO-12400,POB B, Ft Detrick, MD 21702 USA. EM jivanic@ncifcrf.gov NR 46 TC 22 Z9 22 U1 0 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1089-5639 J9 J PHYS CHEM A JI J. Phys. Chem. A PD MAR 25 PY 2004 VL 108 IS 12 BP 2314 EP 2323 DI 10.1021/jp031214g PG 10 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA 804UG UT WOS:000220323000020 ER PT J AU Shulenin, S Nogee, LM Annilo, T Wert, SE Whitsett, JA Dean, M AF Shulenin, S Nogee, LM Annilo, T Wert, SE Whitsett, JA Dean, M TI ABCA3 gene mutations in newborns with fatal surfactant deficiency SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article ID CASSETTE TRANSPORTER 1; PROTEIN-B DEFICIENCY; TANGIER-DISEASE; STARGARDT-DISEASE; MEMBRANE-PROTEIN; II CELLS; ACCUMULATION; LUNG; SITOSTEROLEMIA; DYSTROPHY AB BACKGROUND: Pulmonary surfactant forms a lipid-rich monolayer that coats the airways of the lung and is essential for proper inflation and function of the lung. Surfactant is produced by alveolar type II cells, stored intracellularly in organelles known as lamellar bodies, and secreted by exocytosis. The gene for ATP-binding cassette transporter A3 (ABCA3) is expressed in alveolar type II cells, and the protein is localized to lamellar bodies, suggesting that it has an important role in surfactant metabolism. METHODS: We sequenced each of the coding exons of the ABCA3 gene in blood DNA from 21 racially and ethnically diverse infants with severe neonatal surfactant deficiency for which the etiologic process was unknown. Lung tissue from four patients was examined by high-resolution light and electron microscopy. RESULTS: Nonsense and frameshift mutations, as well as mutations in highly conserved residues and in splice sites of the ABCA3 gene were identified in 16 of the 21 patients (76 percent). In five consanguineous families with mutations, each pair of siblings was homozygous for the same mutation and each mutation was found in only one family. Markedly abnormal lamellar bodies were observed by ultrastructural examination of lung tissue from four patients with different ABCA3 mutations, including nonsense, splice-site, and missense mutations. CONCLUSIONS: Mutation of the ABCA3 gene causes fatal surfactant deficiency in newborns. ABCA3 is critical for the proper formation of lamellar bodies and surfactant function and may also be important for lung function in other pulmonary diseases. Since it is closely related to ABCA1 and ABCA4, proteins that transport phospholipids in macrophages and photoreceptor cells, it may have a role in surfactant phospholipid metabolism. C1 NCI, Human Genet Sect, Lab Genom Divers, Frederick, MD 21702 USA. Johns Hopkins Univ, Sch Med, Dept Pediat, Baltimore, MD 21205 USA. Univ Cincinnati, Coll Med, Dept Pediat, Cincinnati, OH USA. Cincinnati Childrens Hosp, Med Ctr, Cincinnati, OH USA. RP Dean, M (reprint author), NCI, Human Genet Sect, Lab Genom Divers, Bldg 560,Rm 21-18, Frederick, MD 21702 USA. EM dean@ncifcrf.gov RI Dean, Michael/G-8172-2012; Annilo, Tarmo/J-2900-2013 OI Dean, Michael/0000-0003-2234-0631; Annilo, Tarmo/0000-0002-9588-3058 FU NHLBI NIH HHS [HL-54703, HL-56387] NR 26 TC 326 Z9 344 U1 1 U2 11 PU MASSACHUSETTS MEDICAL SOC/NEJM PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 25 PY 2004 VL 350 IS 13 BP 1296 EP 1303 DI 10.1056/NEJMoa032178 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA 805VN UT WOS:000220393900007 PM 15044640 ER PT J AU Yanamandra, N Kondraganti, S Srinivasula, SM Gujrati, M Olivero, WC Dinh, DH Rao, JS AF Yanamandra, N Kondraganti, S Srinivasula, SM Gujrati, M Olivero, WC Dinh, DH Rao, JS TI Activation of caspase-9 with irradiation inhibits invasion and angiogenesis in SNB19 human glioma cells SO ONCOGENE LA English DT Article DE caspase 9; radiation; in version angiogenesis; glioma ID BROMODEOXYURIDINE LABELING INDEX; CYTOCHROME-C; IN-VIVO; GLIOBLASTOMA-MULTIFORME; BASEMENT-MEMBRANE; INDUCED APOPTOSIS; MALIGNANT GLIOMA; DEATH; RADIATION; MIGRATION AB Glioblastoma multiforme, the most common brain tumor, typically exhibits markedly increased angiogenesis, which is crucial for tumor growth and invasion. Antiangiogenic strategies based on disruption of the tumor microvasculature have proven effective for the treatment of experimental brain tumors. Here, we have overexpressed human caspase-9 by stable transfection in the SNB19 glioblastoma cell line, which normally expresses low levels of caspase-9. Our studies revealed that overexpression of caspase-9 coupled with radiation has a synergistic effect on the inhibition of glioma invasion as demonstrated by Matrigel assay (465%). Furthermore, sense caspase stable clones cocultured with fetal rat brain aggregates along with radiation showed complete inhibition as compared to the parental and vector controls. During in vitro angiogenesis, SNB19 cells cocultured with human microvascular endothelial cells (HMEC) showed vascular network formation after 48-72 h. In contrast, these capillary-like structures were inhibited when HMEC cells were cocultured with sense caspase stable SNB19 cells. This effect was further enhanced by radiation (5 Gy). Signaling mechanisms revealed that apoptosis is induced by cleavage of caspase-9 by radiation, loss of mitochondrial membrane potential and activation of caspase-3. These results demonstrate that activation of caspase-9 disrupts glioma cell invasion and angiogenesis in vitro. Hence, overexpression of proapoptotic molecules such as caspase-9 may be an important determinant of the therapeutic effect of radiation in cancer therapy. C1 Univ Illinois, Coll Med, Program Canc Biol, Dept Biomed & Therapeut Sci, Peoria, IL 61656 USA. NCI, Lab Immune Cell Biol, NIH, Bethesda, MD USA. Univ Illinois, Coll Med, Dept Pathol, Peoria, IL 61656 USA. Univ Illinois, Coll Med, Dept Neurosurg, Peoria, IL 61656 USA. RP Rao, JS (reprint author), Univ Illinois, Coll Med, Program Canc Biol, Dept Biomed & Therapeut Sci, Box 1649, Peoria, IL 61656 USA. EM jsrao@uic.edu FU NCI NIH HHS [CA 75557, CA 92393, CA 85216, CA 95058]; NINDS NIH HHS [NS 47699] NR 42 TC 14 Z9 14 U1 0 U2 0 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 25 PY 2004 VL 23 IS 13 BP 2339 EP 2346 DI 10.1038/sj.onc.1207406 PG 8 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA 806IN UT WOS:000220427700008 PM 14767475 ER PT J AU Black, EJ Clair, T Delrow, J Neiman, P Gillespie, DAF AF Black, EJ Clair, T Delrow, J Neiman, P Gillespie, DAF TI Microarray analysis identifies Autotaxin, a tumour cell motility and angiogenic factor with lysophospholipase D activity, as a specific target of cell transformation by v-Jun SO ONCOGENE LA English DT Article DE v-Jun; autotaxin; microarray; lysophospholipase; angiogenesis ID HYPOXIA-INDUCIBLE FACTOR-1; ESTROGEN RECEPTOR CHIMERA; C-JUN; TRANSCRIPTIONAL ACTIVATION; GROWTH; GENE; PROTEIN; DNA; FIBROBLASTS; EXPRESSION AB We have used chicken cDNA microarrays to investigate gene-expression changes induced during transformation of chick embryo fibroblasts (CEF) by the viral Jun oncoprotein encoded by ASV17. This analysis reveals that v-Jun induces increases and decreases of varying magnitude in the expression of genes involved in diverse cellular functions, most of which have not been detected in previous screens for putative v-Jun targets. In all, 27 individual genes were identified, whose expression is increased threefold or more in v-Jun-transformed cells, including genes involved in energy generation, protein synthesis, and gene transcription. Interestingly, this group includes the hypoxia-inducible factor-1 alpha (Hif-1alpha) transcription factor and the glycolytic enzyme enolase, suggesting that adaptation to hypoxia could play a role in tumorigenesis by v-Jun. We also identified 32 genes whose expression is decreased threefold or more, including chaperones, components of the cytoskeleton, and, unexpectedly, DNA replication factors. The gene whose expression is upregulated most dramatically (similar to100-fold) encodes Autotaxin (ATX), a secreted tumor motility-promoting factor with lysophospholipase D activity. Strikingly, v-Jun-transformed CEF secrete catalytically active ATX and chemotactic activity, which can be detected in conditioned medium. ATX is not detectably expressed in normal CEF or CEF transformed by the v-Src or v-Myc oncoproteins, indicating that induction of this putative autocrine/paracrine factor is a specific consequence of cell transformation by v-Jun. ATX has been implicated in both angiogenesis and invasion, and could therefore play an important role in tumorigenesis by v-Jun in vivo. C1 Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland. Beatson Inst Canc Res, Canc Res Campaign Beatson Labs, Glasgow G61 1BD, Lanark, Scotland. NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA. Fred Hutchinson Canc Res Ctr, Dept Human Biol, Seattle, WA 98109 USA. RP Gillespie, DAF (reprint author), Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland. EM d.gillespie@beatson.gla.ac.uk FU NCI NIH HHS [R01 CA20068] NR 46 TC 50 Z9 51 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 25 PY 2004 VL 23 IS 13 BP 2357 EP 2366 DI 10.1038/sj.onc.1207377 PG 10 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA 806IN UT WOS:000220427700010 PM 14691447 ER PT J AU Agarwal, RK Viley, A Silver, P Grajewski, R Kronenberg, M Murray, P Rutschman, R Chan, CC Caspi, R AF Agarwal, RK Viley, A Silver, P Grajewski, R Kronenberg, M Murray, P Rutschman, R Chan, CC Caspi, R TI Transgenic expression of IL-10 targeted to macrophages protects from induction of Experimental Autoimmune Uveitis (EAU) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Annual Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. Univ Calif Los Angeles, Dept Microbiol & Immunol, Los Angeles, CA 90024 USA. St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38101 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1170 EP A1170 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701965 ER PT J AU Ahmadzadeh, M Rosenberg, SA AF Ahmadzadeh, M Rosenberg, SA TI Transforming Growth Factor-beta 1 attenuates the differentiation and effector function of tumor antigen specific human memory CD8 T cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1151 EP A1151 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701874 ER PT J AU Allen, JC Askari, A Liu, LJ Abramowitz, J AF Allen, JC Askari, A Liu, LJ Abramowitz, J TI Low concentrations of cardiotonic steroids activate vascular smooth muscle cell (vsmc) proliferation by interaction with caveolar localized Na pumps SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Baylor Coll Med, Houston, TX 77030 USA. Med Coll Ohio, Toledo, OH 43699 USA. NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1024 EP A1024 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701267 ER PT J AU Aslamkhan, AG Barros, SA Wolff, NA Miller, DS Pritchard, JB AF Aslamkhan, AG Barros, SA Wolff, NA Miller, DS Pritchard, JB TI Basolateral organic anion transport in Fundulus hereroclitus (killifish) proximal tubules appears to be mediated by a single OAT SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIEHS, Res Triangle Pk, NC 27709 USA. Univ Gottingen, D-3400 Gottingen, Germany. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1250 EP A1250 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702345 ER PT J AU Azmi, H Fariss, R Seko, Y Ragheb, JA AF Azmi, H Fariss, R Seko, Y Ragheb, JA TI Selective cytoplasmic translocation of HuR and site specific binding to the IL-2 mRNA are not sufficient for CD28-mediated stabilization of the IL-2 mRNA SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. NEI, Biol Imaging Core, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A807 EP A807 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700231 ER PT J AU Bae, MK Kidd, K Torres-Vazquez, J Kamei, M Pine, H Berk, J McElwain, M Weinstein, B AF Bae, MK Kidd, K Torres-Vazquez, J Kamei, M Pine, H Berk, J McElwain, M Weinstein, B TI An F3 genetic screen for vascular-specific mutants using transgenic zebrafish expressing EGFP in blood vessels SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NICHD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A788 EP A788 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700132 ER PT J AU Bauer, AK Dixon, D DeGraff, LM Malkinson, AM Kleeberger, SR AF Bauer, AK Dixon, D DeGraff, LM Malkinson, AM Kleeberger, SR TI A protective role for toll-like receptor 4 (TLR4) in butylated hydroxytoluene (BHT)-induced lung inflammation and lymphocytic nodule formation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIEHS, Res Triangle Pk, NC 27709 USA. Univ Colorado, Hlth Sci Ctr, Denver, CO USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1158 EP A1158 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701907 ER PT J AU Beaulieu, LM Elkahloun, A Whitley, BR Palmieri, D Church, F AF Beaulieu, LM Elkahloun, A Whitley, BR Palmieri, D Church, F TI Protein C inhibitor regulates inflammatory and apoptotic genes in MDA-MB-435 cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA. NHGRI, Genome Technol Branch, NIH, Bethesda, MD 20892 USA. Univ N Carolina, Div Hematol Oncol Med, Chapel Hill, NC 27515 USA. RI Palmieri, Diane/B-4258-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1195 EP A1195 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702084 ER PT J AU Belyakov, IM Klinman, D Kuznetsov, VA Moniuszko, M Ahlers, JD Kelsall, B Strober, W Franchini, G Berzofsky, JA AF Belyakov, IM Klinman, D Kuznetsov, VA Moniuszko, M Ahlers, JD Kelsall, B Strober, W Franchini, G Berzofsky, JA TI Progress on new mucosal vaccine strategies for HIV SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Metab Branch, Bethesda, MD 20892 USA. NICHD, Lab Integrat & Med Biophys, Bethesda, MD 20892 USA. NCI, Basic Res Lab, Bethesda, MD 20892 USA. NIAID, Clin Invest Lab, Bethesda, MD 20892 USA. US FDA, Ctr Biol Evaluat & Res, Bethesda, MD USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A821 EP A821 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700302 ER PT J AU Block, EM Fenton, JI Laird, J Shin, H Ustunol, Z Pestka, JJ Hord, NG AF Block, EM Fenton, JI Laird, J Shin, H Ustunol, Z Pestka, JJ Hord, NG TI Modulation of Escherichia coli : O157 : H7-mediated production of proinflammatory mediators by two species of Lactobacilli in conditionally immortal colon epithelial cell lines contrasting in adenomatous polyposis coli(Apc)genotype SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Michigan State Univ, E Lansing, MI 48824 USA. NCI, Div Canc Prevent, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A888 EP A888 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700619 ER PT J AU Bolland, S Kole, HK Chi, AWS AF Bolland, S Kole, HK Chi, AWS TI T cell-specific deletion of SHIP results in peripheral unresponsiveness and diminished positive selection SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A812 EP A812 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700256 ER PT J AU Bow, DAJ Perry, JL Simon, JD Pritchard, JB AF Bow, DAJ Perry, JL Simon, JD Pritchard, JB TI Effects of serum albumin on the renal handling of the fungal mycotoxin, Ochratoxin A SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIEHS, Lab Pharmacol & Chem, Res Triangle Pk, NC 27709 USA. Duke Univ, Dept Chem, Durham, NC 27706 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1249 EP A1250 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702344 ER PT J AU Brescia, AC Thurber, DB Fischer, RT Hurt, E Staudt, L Phillips, TM Lipsky, PE Grammer, AC AF Brescia, AC Thurber, DB Fischer, RT Hurt, E Staudt, L Phillips, TM Lipsky, PE Grammer, AC TI Multiparameter genomic/proteomic identification of genes that characterize Ig-secreting B cells in human secondary lymphoid tissue or the periphery of active SLE patients SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, NIH, Autoimmun Branch, Bethesda, MD 20892 USA. NCI, NIH, Bethesda, MD 20892 USA. NIH, OD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A840 EP A840 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700393 ER PT J AU Cai, Q Ferraris, JD Burg, MB AF Cai, Q Ferraris, JD Burg, MB TI High NaCl increases TonEBP/OREBP mRNA and protein by stabilizing its mRNA SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHLBI, NIH, Bethesda, MD 20892 USA. NIH, Kidney & Electrolyte Metab Lab, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1283 EP A1283 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702501 ER PT J AU Caldwell, SA Abrams, SI AF Caldwell, SA Abrams, SI TI Mechanisms of tumor regression by CTL adoptive transfer against extensive lung metastasis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, LTIB, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1152 EP A1152 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701879 ER PT J AU Chatterjie, N Stables, JP Wang, H Alexander, GJ AF Chatterjie, N Stables, JP Wang, H Alexander, GJ TI Modafinil, anti-narcoleptic and potentially anti-epileptic drug: novel synthesis and properties SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 New York State Inst Basic Res Dev Disabil, Staten Isl, NY 10314 USA. NINDS, Preclin Screening Program, Bethesda, MD 20892 USA. CUNY Coll Staten Isl, Staten Isl, NY USA. New York State Inst Basic Res Dev Disabil, Staten Isl, NY 10314 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A964 EP A964 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700977 ER PT J AU Chen, Q Wade, D Kurosaka, K Oppenheim, JJ Yang, D AF Chen, Q Wade, D Kurosaka, K Oppenheim, JJ Yang, D TI Temporin A, a frog antimicrobial peptide, uses human FPRL1/murine FPR2 as a receptor to chemoattract phagocytes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA. Rutgers State Univ, Dept Chem & Chem Biol, Piscataway, NJ USA. SAIC, Mol Immunoregulat Lab, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1147 EP A1147 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701854 ER PT J AU Chen, WJ Jin, WW Hardegen, N Li, L Wahl, SM AF Chen, WJ Jin, WW Hardegen, N Li, L Wahl, SM TI TGF-beta induces Foxp3 in CD4+CD25- naive T cells with conversion to CD4+CD25+ regulatory T cells suppressing antigen-specific CD4+ T cell expansion in vivo and HDM asthma SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIDCR, OIIB, CIS, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A799 EP A799 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700193 ER PT J AU Chipman, J Metter, EJ Conwit, R Abernethy, DR Ling, SM AF Chipman, J Metter, EJ Conwit, R Abernethy, DR Ling, SM TI Surface EMG signals in osteoarthritis of the knee: a pilot study SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, Clin Res Branch, Baltimore, MD 21225 USA. NINDS, Clin Res Branch, Bethesda, MD 20892 USA. NIA, Clin Invest Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1095 EP A1096 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701606 ER PT J AU Chou, CL Christensen, BM Frische, S Vorum, H Desai, R Hoffert, JD Nielsen, S Knepper, MA AF Chou, CL Christensen, BM Frische, S Vorum, H Desai, R Hoffert, JD Nielsen, S Knepper, MA TI Vasopressin induces phosphorylation of myosin light chain SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHLBI, NIH, Bethesda, MD 20892 USA. Univ Aarhus, Walter & Salt Res Ctr, DK-8000 Aarhus C, Denmark. RI Frische, Sebastian/B-2332-2009 OI Frische, Sebastian/0000-0002-0270-3602 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1019 EP A1020 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701244 ER PT J AU Conley, M Silva, P Satpute-Krishnan, P Ferland, P DeGiorgis, JA Santos-McClure, E Bearer, EL AF Conley, M Silva, P Satpute-Krishnan, P Ferland, P DeGiorgis, JA Santos-McClure, E Bearer, EL TI Molecular and functional interactions of herpes simplex virus with the amyloid precursor protein of Alzheimer's disease: Evidence for a causal role for herpes in Alzheimer's dementia SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Brown Med Sch, Providence, RI 02912 USA. NINDS, NIH, Bethesda, MD 20892 USA. Brown Univ, Brown MBL, Providence, RI 02912 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A933 EP A933 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700831 ER PT J AU Dmitrieva, NI Cai, Q Burg, MB AF Dmitrieva, NI Cai, Q Burg, MB TI The high NaCl normally present in renal inner medulla induces DNA breaks and decreases DNA repair in vivo SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHLBI, LKEM, DHHS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1020 EP A1020 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701247 ER PT J AU Dobbins, DE Wilder, RL Remmers, EF AF Dobbins, DE Wilder, RL Remmers, EF TI Radiation hybrid mapping of a disease severity locus for collagen-induced arthritis, an animal model for rheumatoid arthritis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. Medimmune Inc, Clin Dev, Gaithersburg, MD 20878 USA. NIAMS, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A781 EP A781 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700102 ER PT J AU Ebert, SN Rong, Q Boe, S Thompson, RP Pfeifer, K AF Ebert, SN Rong, Q Boe, S Thompson, RP Pfeifer, K TI Intrinsic cardiac adrenergic cells as novel progenitors of pacemaking and conduction system myocytes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Georgetown Univ, Med Ctr, Washington, DC 20057 USA. NICHD, Lab Mammalian Genes & Dev, NIH, Bethesda, MD USA. Med Univ S Carolina, Charleston, SC 29425 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A783 EP A783 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700111 ER PT J AU Fairhurst, AM Ishii, KJ Porter, MA Siegel, RM Lipsky, PE Ettinger, R AF Fairhurst, AM Ishii, KJ Porter, MA Siegel, RM Lipsky, PE Ettinger, R TI TLR9 and TNF deficiency: a role in autoimmunity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, NIH, Bethesda, MD 20892 USA. Osaka Univ, Dept Host Def, Osaka, Japan. RI Ishii, Ken/B-1685-2012 OI Ishii, Ken/0000-0002-6728-3872 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1162 EP A1162 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701927 ER PT J AU Fenton, RA Chou, CL Stewart, GS Smith, CP Knepper, MA AF Fenton, RA Chou, CL Stewart, GS Smith, CP Knepper, MA TI Urinary concentrating defect in mice with selective deletion of phloretin-sensitive urea transporters in the renal collecting duct SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHLBI, LKEM, NIH, Bethesda, MD 20892 USA. Univ Manchester, Sch Biol Sci, Manchester M13 9PL, Lancs, England. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1018 EP A1018 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701237 ER PT J AU Fischer, RT Sohn, HW Longo, NS Yarboro, C Illei, G Pierce, S Lipsky, PE Grammer, AC AF Fischer, RT Sohn, HW Longo, NS Yarboro, C Illei, G Pierce, S Lipsky, PE Grammer, AC TI Functional and phenotypic characterization of anergic B cells circulating in the periphery of active SLE patients SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, B Cell Biol Grp, NIH, Bethesda, MD 20892 USA. NIAID, LIG, NIH, Bethesda, MD 20892 USA. NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A839 EP A839 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700387 ER PT J AU Fritsch, RD Olson, D Illei, G Yarboro, C Lipsky, PE AF Fritsch, RD Olson, D Illei, G Yarboro, C Lipsky, PE TI Characterization of differences in memory T cell subsets in SLE patients and Healthy Controls SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. NIAMS, Off Clin Director, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1133 EP A1133 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701787 ER PT J AU Fueger, PT Heikkinen, S Bracy, DP Malabanan, CM Pencek, RR Laakso, M Wasserman, DH AF Fueger, PT Heikkinen, S Bracy, DP Malabanan, CM Pencek, RR Laakso, M Wasserman, DH TI Hexokinase II partial knockout creates exercise intolerance and exercise-induced hyperglycemia in C57BL/6J mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Vanderbilt Univ, Mouse Metab Phenotyping Ctr, Nashville, TN 37232 USA. Univ Kuopio, FIN-70211 Kuopio, Finland. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1224 EP A1224 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702224 ER PT J AU Gallagher, D Albu, J Heymsfield, S Heshka, S Kuznia, P Goodpaster, B Visser, M Harris, T AF Gallagher, D Albu, J Heymsfield, S Heshka, S Kuznia, P Goodpaster, B Visser, M Harris, T TI Intermuscular adipose tissue and its relationship to metabolic risk factors SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Columbia Univ, St Lukes Roosevelt Hosp, Obes Res Ctr, New York, NY 10025 USA. NIA, Geriatr Epidemiol Sect, Bethesda, MD 20892 USA. Univ Pittsburgh, Dept Med, Pittsburgh, PA 15260 USA. VUMC, Amsterdam, Netherlands. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1112 EP A1113 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701688 ER PT J AU Garcia, EE Lai, ZN Barret, RJ Sanders-Bush, E AF Garcia, EE Lai, ZN Barret, RJ Sanders-Bush, E TI Unraveling the role of G-proteins in hallucinogenic drug action SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Vanderbilt Univ, Dept Psychiat, Nashville, TN 37232 USA. NINDS, Dev & Metab Neurol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A973 EP A973 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701018 ER PT J AU Gattinoni, L Finkelstein, SE Klebanoff, CA Surman, DR Wrzesinski, C Palmer, DC Heimann, DM Spiess, PJ Rosenberg, SA Restifo, NP AF Gattinoni, L Finkelstein, SE Klebanoff, CA Surman, DR Wrzesinski, C Palmer, DC Heimann, DM Spiess, PJ Rosenberg, SA Restifo, NP TI When good T cells go bad: acquisition of terminal effector function in vitro impairs in vivo anti-tumor efficacy SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, NIH, Surg Branch, Bethesda, MD 20892 USA. RI Restifo, Nicholas/A-5713-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1154 EP A1155 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701890 ER PT J AU Gonsky, R Deem, RL Bream, JH Young, H Targan, SR AF Gonsky, R Deem, RL Bream, JH Young, H Targan, SR TI Enhancer role of STAT5 in CD2 activation of IFN-gamma gene expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Cedars Sinai Med Ctr, IBD Ctr, Los Angeles, CA 90048 USA. NIAMS, NIH, Bethesda, MD USA. NCI, Frederick Canc Res & Dev Ctr, Expt Immunol Lab, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1137 EP A1137 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701807 ER PT J AU Grajewski, RS Silver, PB Agarwal, RK Chan, CC Caspi, RR AF Grajewski, RS Silver, PB Agarwal, RK Chan, CC Caspi, RR TI Thymus-derived CD4+CD25+regulatory T cells set the threshold of susceptibility to Experimental Autoimmune Uveitis (EAU) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A832 EP A832 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700353 ER PT J AU Granvil, CP Gonzalez, FJ AF Granvil, CP Gonzalez, FJ TI P450 humanized mice for the study of xenobiotics: CYP3A4 as a paradigm SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Lab Metab, NIH, Bethesda, MD 20892 USA. CombinatoRx Inc, Drug Dev Dept, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1201 EP A1201 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702113 ER PT J AU Haeryfar, SMM DiPaolo, RJ Tscharke, DC Bennink, JR Yewdell, JW AF Haeryfar, SMM DiPaolo, RJ Tscharke, DC Bennink, JR Yewdell, JW TI CD4+CD25+ regulatory T cells do not influence immunodominance hierarchies of virus-specific TCD8+ cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, NIH, Viral Dis Lab, Bethesda, MD 20892 USA. NIAID, NIH, Immunol Lab, Bethesda, MD 20892 USA. Queensland Inst Med Res, EBV Unit, Herston, Qld 4006, Australia. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1143 EP A1143 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701836 ER PT J AU Holvoet, P Kritchevsky, SB Tracy, RP Mertens, A Rubin, SM Butler, J Goodpaster, B Harris, TB AF Holvoet, P Kritchevsky, SB Tracy, RP Mertens, A Rubin, SM Butler, J Goodpaster, B Harris, TB TI The metabolic syndrome, circulating oxidized LDL and risk of myocardial infarction in well-functioning elders in the Health ABC cohort SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Katholieke Univ Leuven, B-3000 Louvain, Belgium. Wake Forest Univ, Sch Med, Winston Salem, NC 27109 USA. Univ Vermont, Burlington, VT 05405 USA. Univ Calif San Francisco, San Francisco, CA 94143 USA. Vanderbilt Univ, Nashville, TN USA. Univ Pittsburgh, Pittsburgh, PA 15260 USA. NIA, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1191 EP A1191 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702067 ER PT J AU Hoodbhoy, T Dean, J AF Hoodbhoy, T Dean, J TI Reassessing the molecular biology of sperm-egg recognition with mouse genetics SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIDDK, LCDB, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1124 EP A1124 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701742 ER PT J AU Hu, AH Lai, ZN Zhang, J Wang, MY Xiao, RP Ma, XL AF Hu, AH Lai, ZN Zhang, J Wang, MY Xiao, RP Ma, XL TI Lentiviral vectors efficiently deliver and express genes in adult rat cardiovascular system SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Thomas Jefferson Univ, Philadelphia, PA 19107 USA. NINDS, Dev & Metab Neurol Branch, Bethesda, MD 20892 USA. NIA, Cardiovasc Sci Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1014 EP A1014 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701216 ER PT J AU Hu, PQ Oriss, T Medsger, T Wright, T AF Hu, PQ Oriss, T Medsger, T Wright, T TI Autoreactive T cells recognize multiple epitopes on DNA topoisomerase I in sytemic sclerosis patients and in healthy controls SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA. Univ Pittsburgh, Dept Med, Pittsburgh, PA 15260 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A836 EP A836 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700372 ER PT J AU Huang, LYC Ishii, KJ Bafica, A Akira, S Sher, A Aliberti, J Golding, B AF Huang, LYC Ishii, KJ Bafica, A Akira, S Sher, A Aliberti, J Golding, B TI Toll-like receptor 9 is required for IL-12 induction in response to bacterial stimulation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 CBER FDA, Div Hematol, Rockville, MD 20852 USA. Osaka Univ, RIMD, Suita, Osaka 565, Japan. NIAID, LPD, NIH, Bethesda, MD 20892 USA. Duke Univ, Med Ctr, Dept Immunol, Durham, NC 27706 USA. RI Aliberti, Julio/I-7354-2013; Ishii, Ken/B-1685-2012 OI Aliberti, Julio/0000-0003-3420-8478; Ishii, Ken/0000-0002-6728-3872 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1157 EP A1157 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701903 ER PT J AU Irarrazabal, CE Burg, MB Ferraris, JD AF Irarrazabal, CE Burg, MB Ferraris, JD TI ATM is activated by high NaCl, contributing to increased TonEBP/OREBP-mediated transcription SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIH, LKEM, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1283 EP A1283 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702502 ER PT J AU Iwaki, S Tkaczyk, C Satterthwaite, AB Draber, P Horejsi, V Metcalfe, DD Gilfillan, AM AF Iwaki, S Tkaczyk, C Satterthwaite, AB Draber, P Horejsi, V Metcalfe, DD Gilfillan, AM TI NTAL (LAB) phosphorylation is differentially regulated by antigen and stem cell factor (SCF) in mast cells (MC) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75230 USA. Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75230 USA. Acad Sci Czech Republ, Inst Mol Genet, Prague, Czech Republic. RI Horejsi, Vaclav/G-3113-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A791 EP A791 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700151 ER PT J AU Jameson, RR Carlson, BA Hatfield, DL Diamond, AM AF Jameson, RR Carlson, BA Hatfield, DL Diamond, AM TI Methylation of tRNA[Ser]Sec in the regulation of selenoprotein synthesis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Illinois, Chicago, IL 60612 USA. NCI, Sect Mol Biol Selenium, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A850 EP A850 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700439 ER PT J AU Jiang, YC Liu, J Cheng, ML Kang, YJ AF Jiang, YC Liu, J Cheng, ML Kang, YJ TI Inhibition of hepatic stellate cell activation by a Chinese medicine preparation Han-Dan-Gan-Le SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Louisville, Sch Med, Louisville, KY 40202 USA. NCI, NIEHS, Res Triangle Pk, NC USA. Guiyang Med Coll, Guiyang, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A938 EP A938 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700852 ER PT J AU Jones, LP Lubet, R Grubbs, C Furth, PA AF Jones, LP Lubet, R Grubbs, C Furth, PA TI Estrogen pathway signaling in Brca1 deficient mammary glands SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Georgetown Univ, Vincent T Lombardi Canc Res Ctr, Washington, DC 20057 USA. NCI, Div Chemoprotect, Bethesda, MD 20892 USA. Univ Alabama, Chemoprevent Ctr, Birmingham, AL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A936 EP A936 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700846 ER PT J AU Jozwiak, K Moaddel, R Ravichandran, S Collins, JR Wainer, IW AF Jozwiak, K Moaddel, R Ravichandran, S Collins, JR Wainer, IW TI A chromatographic and chemometric approach to the identification of non-competitive inhibitors of the nicotinic acetylcholine receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, Gerontol Res Ctr, LCI, NIH, Baltimore, MD 21224 USA. NCI, SAIC, ABCC, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A966 EP A967 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700988 ER PT J AU Kamei, M Bayless, KJ Davis, GE Weinstein, BM AF Kamei, M Bayless, KJ Davis, GE Weinstein, BM TI Real-time in vivo imaging of endothelial lumen formation in the zebrafish Danio rerio SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC ID CAPILLARY LUMEN C1 NICHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. Texas A&M Univ Syst, Hlth Sci Ctr, Dept Pathol & Lab Med, College Stn, TX USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A787 EP A787 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700131 ER PT J AU Karnaky, K Daniels, J Tully, S Jones, L Miller, DS AF Karnaky, K Daniels, J Tully, S Jones, L Miller, DS TI MRP2-like transport model system from cricket, Acheta domesticus, Malpighian tubules SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Med Univ S Carolina, Charleston, SC 29425 USA. Coll Charleston, Charleston, SC 29401 USA. NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1305 EP A1305 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702606 ER PT J AU Kidd, KR Greer, K Hoying, JB Weinstein, BM AF Kidd, KR Greer, K Hoying, JB Weinstein, BM TI Molecular analysis of arterial-venous differentiation using oligonucleotide microarray technology in the zebrafish SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NICHD, NIH, Bethesda, MD 20892 USA. Univ Arizona, Tucson, AZ 85721 USA. NR 0 TC 0 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A776 EP A776 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700077 ER PT J AU King-Pospisil, KA Chalimoniuk, M Pedersen, WA Mattson, MP Hennig, B Toborek, M AF King-Pospisil, KA Chalimoniuk, M Pedersen, WA Mattson, MP Hennig, B Toborek, M TI Protein Kinase C is involved in arachidonic acid-induced choline acetyltransferase activity in spinal cord neurons SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Kentucky, Dept Surg, Lexington, KY 40536 USA. Polish Acad Sci, Dept Cellular Signaling, Warsaw, Poland. Creighton Univ, Ctr Aging, Omaha, NE 68178 USA. NIA, NIH, Baltimore, MD 21224 USA. RI Mattson, Mark/F-6038-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1200 EP A1200 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702109 ER PT J AU Krijger, PHL van Laar, JM Satorius, C Lipsky, PE AF Krijger, PHL van Laar, JM Satorius, C Lipsky, PE TI B lymphocyte repertoire in rheumatoid arthritis after high dose immunosuppresive therapy and autologous stem cell transplantation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A839 EP A840 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700390 ER PT J AU Kurosaka, K Chen, Q Oppenheim, JJ Yang, D AF Kurosaka, K Chen, Q Oppenheim, JJ Yang, D TI CRAMP, the mouse cathelicidin, uses FPRL1/mFPR2 as the receptor to chemoattract leukocytes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA. SAIC, Mol Immunoregulat Lab, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1147 EP A1147 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701853 ER PT J AU Kutty, V Nagineni, CN Detrick, B Hooks, JJ AF Kutty, V Nagineni, CN Detrick, B Hooks, JJ TI Differential expression of PDGF and their receptors in human retinal pigment epithelial and choroidal cells: Role in proliferative vitreoretinopathy SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Dept Pathol, Baltimore, MD 21287 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1118 EP A1118 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701714 ER PT J AU Lam, AKM Ko, BCB Tam, SCF Knepper, MA Morris, R Yang, JY Chung, SK Chung, SSM AF Lam, AKM Ko, BCB Tam, SCF Knepper, MA Morris, R Yang, JY Chung, SK Chung, SSM TI Osmotic Response Element Binding Protein (OREBP) is essential for water reabsorption in kidney SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Hong Kong, Inst Mol Biol, Hong Kong, Peoples R China. Univ Hong Kong, Dept Clin Biochem, Hong Kong, Peoples R China. NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1020 EP A1020 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701246 ER PT J AU Leon, F Contractor, N Fuss, I Marth, T Lahey, E Iwaki, S la Sala, A Strober, W Kelsall, BL AF Leon, F Contractor, N Fuss, I Marth, T Lahey, E Iwaki, S la Sala, A Strober, W Kelsall, BL TI Antibodies to CR3 (CD11b/Mac-1) treat established inflammation in a novel murine model of combined inflammatory bowel and psoriatic skin disease. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Clin Invest Lab, NIH, Bethesda, MD 20892 USA. NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. RI la Sala, Andrea/A-3228-2009 OI la Sala, Andrea/0000-0003-1268-6516 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1171 EP A1171 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701971 ER PT J AU Li, CL Shi, YM Wang, WD Sardeli, C Kwon, TH Thomsen, K Jonassen, TEN Knepper, MA Nielsen, S Frokiaer, J AF Li, CL Shi, YM Wang, WD Sardeli, C Kwon, TH Thomsen, K Jonassen, TEN Knepper, MA Nielsen, S Frokiaer, J TI Alpha-MSH prevents dysregulation of AQPs and Na,K-ATPase and impairment in renal function in rats with bilateral ureteral obstruction (BUO) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Walter & Salt Res Ctr, DK-8200 Aarhus, Denmark. Inst Basic Psychiat Res, Dept Biol Psychiat, Risskov, Denmark. NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bethesda, MD 20892 USA. Univ Copenhagen, Dept Pharmacol, DK-1168 Copenhagen, Denmark. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1019 EP A1019 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701241 ER PT J AU Lieto, LD Borrego, F You, C Coligan, J AF Lieto, LD Borrego, F You, C Coligan, J TI Human CD94 gene expression: Dual promoters that differ in responsiveness to IL-2 or IL-15 and bind STAT5A and STAT5B SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, LAD, RCBS, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A814 EP A814 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700265 ER PT J AU Longo, NS Bleesing, JH Dale, JK Fleisher, TA Straus, SE Lipsky, PE AF Longo, NS Bleesing, JH Dale, JK Fleisher, TA Straus, SE Lipsky, PE TI Abnormal selection and B cell memory in human Fas deficiency SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. Univ Arkansas Med Sci, Arkansas Childrens Hosp, Res Inst, Little Rock, AR 72205 USA. NIAID, Clin Invest Lab, Bethesda, MD 20892 USA. NIAID, Warren G Magnuson Clin Ctr, Bethesda, MD 20892 USA. NCCAM, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A834 EP A834 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700364 ER PT J AU Lopaczynski, W Gregory, S Zhang, YP Cox, JH Koup, R D'Souza, P Lathey, JL AF Lopaczynski, W Gregory, S Zhang, YP Cox, JH Koup, R D'Souza, P Lathey, JL TI Optimization of the ELISpot assay for use in vaccine immunotherapy trials SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 BBI Biotech Res Labs, Gaithersburg, MD 20877 USA. US Mil HIV Res Program, HMJF, Rockville, MD USA. NIAID, VRC, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A801 EP A801 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700203 ER PT J AU Lumsden, J Shen, RN Petiniot, L McCarty, T Barlow, C Wynn, T Morse, H Wynshaw-Boris, A Max, E Hodes, R AF Lumsden, J Shen, RN Petiniot, L McCarty, T Barlow, C Wynn, T Morse, H Wynshaw-Boris, A Max, E Hodes, R TI Immunoglobulin class switch recombination is impaired in Atm-deficient mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIH, Expt Immunol Branch, Bethesda, MD 20892 USA. NIAID, NIH, Bethesda, MD 20892 USA. NCHGR, NIH, Bethesda, MD USA. US FDA, Ctr Biol Evaluat & Res, Bethesda, MD USA. RI Lumsden, Joanne/B-6475-2009 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1132 EP A1132 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701782 ER PT J AU Marusina, AI Kim, DK Lieto, L Borrego, F Coligan, JE AF Marusina, AI Kim, DK Lieto, L Borrego, F Coligan, JE TI GATA-3 is an important transcription factor for regulating NKG2A gene expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, LAD, NIH, Rockville, MD 20852 USA. Chonbuk Natl Univ, Sch Med, Dept Immunol, Chonju, South Korea. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A815 EP A815 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700273 ER PT J AU Mattapallil, MJ Viley, AM Chan, CC Silver, PB Donoso, LA Hanson, JA David, CS Caspi, RR AF Mattapallil, MJ Viley, AM Chan, CC Silver, PB Donoso, LA Hanson, JA David, CS Caspi, RR TI HLA DR and DQ interact to control susceptibility to experimental autoimmune uveitis (EAU) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Bethesda, MD 20892 USA. Will Eye Hosp, Dept Biochem & Mol Biol, Philadelphia, PA USA. Mayo Clin, Dept Immunol, Rochester, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A842 EP A842 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700400 ER PT J AU Mentink-Kane, MM Cheever, AW Thompson, RW Hari, DM Jones, FM Donaldson, DD Grusby, MJ Dunne, DW Wynn, TA AF Mentink-Kane, MM Cheever, AW Thompson, RW Hari, DM Jones, FM Donaldson, DD Grusby, MJ Dunne, DW Wynn, TA TI The decoy IL-13 receptor downmodulates granulomatous inflammation and promotes host survival in chronic schistosomiasis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, NIH, Bethesda, MD 20892 USA. BRI, Schistosomiasis Lab, Rockville, MD USA. Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England. Wyeth Ayerst Res, Dept Resp Dis, Cambridge, MA USA. Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. RI Wynn, Thomas/C-2797-2011 NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1140 EP A1140 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701818 ER PT J AU Min, B Foucras, G Meier-Scheilersheim, M Paul, W AF Min, B Foucras, G Meier-Scheilersheim, M Paul, W TI Competition between T cells with related TCR specificity regulates homeostatic proliferation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. Hop Purpan, Ctr Physiopathol Toulouse Purpan, Toulouse, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A796 EP A796 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700177 ER PT J AU Moaddel, R Jozwiak, K Wainer, IW AF Moaddel, R Jozwiak, K Wainer, IW TI The identification of verapamil and verapamil metabolites as non-competitive inhibitors of the nicotinic acetylcholine receptor using chromatographic and chemometric techniques SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, Gerontol Res Ctr, LCI, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A966 EP A966 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700987 ER PT J AU Muppidi, JR Siegel, RM AF Muppidi, JR Siegel, RM TI Ligand-independent redistribution of Fas/CD95 into lipid rafts mediates clonotypic T cell suicide SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A795 EP A795 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700171 ER PT J AU Nagase, H Fogarty, T Arora, M Shanley, K Ward, JM Franklin, C Kullberg, MC Noben-Trauth, N AF Nagase, H Fogarty, T Arora, M Shanley, K Ward, JM Franklin, C Kullberg, MC Noben-Trauth, N TI Roles for both IL-4R alpha and IL-10 in controlling inflammation in a model of inflammatory bowel disease SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 George Washington Univ, Washington, DC 20037 USA. NCI, Ft Detrick, MD 21702 USA. Univ Missouri, Columbia, MO 65211 USA. NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A818 EP A818 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700285 ER PT J AU Nahirney, PC Gutierrez-Cruz, G Lewis, MK Wang, K AF Nahirney, PC Gutierrez-Cruz, G Lewis, MK Wang, K TI Cytoarchitecture of a superfast, high endurance sonic muscle fiber in the type I male midshipman fish SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A784 EP A784 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700114 ER PT J AU Nascimbeni, M Shin, EC Chiriboga, L Kleiner, DE Rehermann, B AF Nascimbeni, M Shin, EC Chiriboga, L Kleiner, DE Rehermann, B TI Peripheral CD4/CD8 double-positive T cells are hepatitis C virus-specific effector memory cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. NYU, Sch Med, Bellevue Hosp, Dept Pathol, New York, NY USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A804 EP A804 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700217 ER PT J AU OHanlon, T Koneru, B Bayat, E Pandey, J Targoff, I Malley, J Malley, K Miller, F AF OHanlon, T Koneru, B Bayat, E Pandey, J Targoff, I Malley, J Malley, K Miller, F TI Genotypic differences between women who develop myositis with and without silicone implants SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIEHS, NIH, Bethesda, MD 20892 USA. Med Univ S Carolina, Charleston, SC 29425 USA. Univ Oklahoma, Hlth Sci Ctr, OMRF, Oklahoma City, OK USA. NIH, CIT, Bethesda, MD 20892 USA. Malley Res Program Inc, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A843 EP A843 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700405 ER PT J AU Park, JM Terabe, M van den Broeke, LT Donaldson, DD Berzofsky, JA AF Park, JM Terabe, M van den Broeke, LT Donaldson, DD Berzofsky, JA TI Unmasking immunosurveillance against a syngeneic colon cancer by elimination of CD4+NKT regulatory cells and IL-13 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Mol Immunogenet & Vaccine Res Sect, NIH, Bethesda, MD 20892 USA. Wyeth Ayerst Res, Discovery Med Inflammat, Cambridge, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1153 EP A1154 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701885 ER PT J AU Patterson, B Wastney, ME Combs, GF Brindak, M Veillon, C Taylor, P Patterson, K Levander, OA AF Patterson, B Wastney, ME Combs, GF Brindak, M Veillon, C Taylor, P Patterson, K Levander, OA TI Selenite and selenomethionine follow a common metabolic pathway in humans SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, DCP, Biometry Res Grp, Bethesda, MD 20892 USA. Metab Modeling Serv Ltd, Modeling, Hamilton, New Zealand. Cornell Univ, Ithaca, NY 14853 USA. USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. NCI, Div Clin Studies, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A849 EP A849 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700436 ER PT J AU Pechhold, KU Harlan, DM AF Pechhold, KU Harlan, DM TI Unique phenotypic features and functional responses of CTL activated by non-professional (np) antigen-presenting cells (APC) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIDDK, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1163 EP A1163 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701930 ER PT J AU Potter, EA Stewart, GS Fenton, RA Smith, CP AF Potter, EA Stewart, GS Fenton, RA Smith, CP TI Characterization of a cDNA encoding a structurally novel urea transporter expressed in human colon SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England. NHLBI, NIH, LKEM, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1270 EP A1270 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470702439 ER PT J AU Puri, RK Bhattacharya, B Miura, T Mejido, J Luo, YQ Yang, AX Joshi, BH Irene, G Rao, M AF Puri, RK Bhattacharya, B Miura, T Mejido, J Luo, YQ Yang, AX Joshi, BH Irene, G Rao, M TI Microrray analysis of gene expression identities unique molecular signature in human embryonic stem cell lines SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 US FDA, Ctr Biol Evaluat & Res, Div Cellular & Gene Therapies, Lab Mol Tumor Biol, Bethesda, MD 20892 USA. NIA, Neurosci Lab, Baltimore, MD 21224 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1121 EP A1121 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701726 ER PT J AU Racanelli, V Behrens, SE Aliberti, J Rehermann, B AF Racanelli, V Behrens, SE Aliberti, J Rehermann, B TI Dendritic cells transfected with cytopathic self-replicating RNA induce crosspriming of HCV-specific CD8+ T cells and antiviral immunity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIDDK, Liver Dis Sect, NIH, Bethesda, MD 20892 USA. Fox Chase Canc Ctr, Virol Program, Philadelphia, PA 19111 USA. NIH, Parasit Dis Lab, Bethesda, MD 20892 USA. RI Aliberti, Julio/I-7354-2013 OI Aliberti, Julio/0000-0003-3420-8478 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A825 EP A825 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700321 ER PT J AU Rai, G Mage, RG O'Neill, TP Newman, BA AF Rai, G Mage, RG O'Neill, TP Newman, BA TI Developing models of autoimmune disease: systemic lupus erythematosus in rabbit SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. Spring Valley Labs, Dept Pathol, Woodbine, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A836 EP A837 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700376 ER PT J AU Rao, MS AF Rao, MS TI Human embryonic stem cells - Basic biology SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, LNS, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1121 EP A1121 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701727 ER PT J AU Sakthivel, SKK Singh, UP Singh, S Palaniappan, R Taub, DD Lillard, JW AF Sakthivel, SKK Singh, UP Singh, S Palaniappan, R Taub, DD Lillard, JW TI Blockade of interferon-gamma inducible protein-10 protects mice from cyclophosphamide-induced cystitis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Morehouse Sch Med, Atlanta, GA 30310 USA. NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1135 EP A1135 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701795 ER PT J AU Scanga, CA Bafica, A Feng, CG Cheever, AW Seder, RA Sher, A AF Scanga, CA Bafica, A Feng, CG Cheever, AW Seder, RA Sher, A TI Impaired pro-inflammatory response to Mycobacterium tuberculosis in MyD88-deficient mice results in profound loss of resistance to infection SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, LPD, NIH, Bethesda, MD 20892 USA. NIAID, VRC, NIH, Bethesda, MD 20892 USA. Biomed Res Inst, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1156 EP A1157 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701900 ER PT J AU Segarra, M Vilardell, C Esparza, J Lozano, E Serra, C Campo, E Yamada, K Cid, MC AF Segarra, M Vilardell, C Esparza, J Lozano, E Serra, C Campo, E Yamada, K Cid, MC TI Dual role of Focal Adhesion Kinase (FAK) in regulating Fibronectin (FN)-induced gelatinase (MMP-2 and MMP-9) production and release by human T lymphoid cell lines SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Barcelona, Hosp Clin, IDIBAPS, E-08036 Barcelona, Spain. NICDR, Craniofacial Dev & Regenerat Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1114 EP A1114 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701694 ER PT J AU Shorts, L Briggs, L Back, T Wiltrout, R AF Shorts, L Briggs, L Back, T Wiltrout, R TI Tumor cell responsiveness to IFN-gamma contributes to IL2/IL12 immunotherapy SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Expt Immunol Lab, CCR, Frederick, MD 21702 USA. Univ Maryland, Dept Biochem, Baltimore, MD 21201 USA. SAIC, Expt Immunol Lab, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1154 EP A1154 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701886 ER PT J AU Silver, P Agarwal, R Su, SB Chan, CC Caspi, R AF Silver, P Agarwal, R Su, SB Chan, CC Caspi, R TI The benefits and pitfalls of protective DNA vaccination against experimental autoimmune uveitis (EAU) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1171 EP A1171 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701972 ER PT J AU Singh, UP Singh, S Taub, DD Lillard, JW AF Singh, UP Singh, S Taub, DD Lillard, JW TI Chronic colitis in IL-10(-/-) mice is mediated in part by IP-10-production from mucosal CD11c(+) dendritic cells and -modulation of CD86 and CD30L expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Morehouse Sch Med, Atlanta, GA 30310 USA. NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1135 EP A1135 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701794 ER PT J AU Snyder, JT Belyakov, IM Dzutsev, A Lemonnier, F Berzofsky, JA AF Snyder, JT Belyakov, IM Dzutsev, A Lemonnier, F Berzofsky, JA TI Protection against lethal virus challenge in HLA-A2 transgenic mice by peptide immunization with an HLA-A0201 restricted CTL epitope of vaccinia and variola SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Metab Branch, NIH, Bethesda, MD 20892 USA. Inst Pasteur, Unite Immunite Cellulaire Antivirale, Paris, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A820 EP A820 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700296 ER PT J AU Song, J Hu, XQ Khan, O Knepper, MA Ecelbarger, CA AF Song, J Hu, XQ Khan, O Knepper, MA Ecelbarger, CA TI Renal bumetanide-sensitive Na-K-2Cl cotransporter (BSC1) and sodium phosphate cotransporter (NaPi-2) are increased in insulin-infused Sprague-Dawley rats SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Georgetown Univ, Dept Med, Washington, DC 20057 USA. NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1015 EP A1016 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701225 ER PT J AU Stapleton, C Jaradat, M Kim, SC Liao, G Cristiano, J Dixon, D Jetten, AM AF Stapleton, C Jaradat, M Kim, SC Liao, G Cristiano, J Dixon, D Jetten, AM TI A protective role for ROR alpha during inflammatory response in the murine lung SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIEHS, Pulm Pathobiol Lab, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A947 EP A947 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700896 ER PT J AU Steinau, M Habis, AH Lee, DR Vernon, SD Ruffin, MT Verma, M Srivastava, S Unger, ER AF Steinau, M Habis, AH Lee, DR Vernon, SD Ruffin, MT Verma, M Srivastava, S Unger, ER TI Molecular signatures of cervical neoplasia: A pilot study of gene expression profiling in exfoliated cervical cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. Univ Michigan, Ann Arbor, MI 48109 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A936 EP A936 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700843 ER PT J AU Stevenson, GW Folk, JE Rice, KC Negus, SS AF Stevenson, GW Folk, JE Rice, KC Negus, SS TI Interactions between the rate-altering, antinociceptive and reinforcing effects of delta and mu opioid agonists in rhesus monkeys: Studies with SNC80 and heroin SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Harvard Univ, McLean Hosp, Sch Med, Belmont, MA 02478 USA. NIDDKD, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A960 EP A960 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700956 ER PT J AU Stewart, TJ Abrams, SI AF Stewart, TJ Abrams, SI TI Alteration in immune responses during development and progression of mouse mammary carcinoma SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Tumor Immunol & Biol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1154 EP A1154 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701887 ER PT J AU Su, SB Silver, P Wang, P Chan, CC Caspi, RR AF Su, SB Silver, P Wang, P Chan, CC Caspi, RR TI Cholera toxin prevents Th1-mediated autoimmune disease by enhancing Th2 polarization SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIH, Immunol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1172 EP A1172 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701975 ER PT J AU Takase, H Yu, CR Mahdi, RM Douek, DC DiRusso, G Midgley, FM Dogra, R Pugliese, A Egwuagu, CE Gery, I AF Takase, H Yu, CR Mahdi, RM Douek, DC DiRusso, G Midgley, FM Dogra, R Pugliese, A Egwuagu, CE Gery, I TI Expression of retinal and melanocyte antigens in human thymi: Variability among antigens and individual thymi SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, NIH, Bethesda, MD 20892 USA. NIAID, NIH, Bethesda, MD 20892 USA. Childrens Natl Med Ctr, Washington, DC 20010 USA. Univ Miami, Diabet Res Inst, Miami, FL 33152 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A833 EP A833 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700360 ER PT J AU Talreja, J Dileepan, K Puri, S Segal, DM Stechschulte, DJ Dileepan, KN AF Talreja, J Dileepan, K Puri, S Segal, DM Stechschulte, DJ Dileepan, KN TI Interferon gamma induces lipopolysaccharide responsiveness in human conjunctival epithelial cells (HCEC) through up-regulation of MD2 expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Kansas, Med Ctr, Kansas City, KS 66160 USA. NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A828 EP A828 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700334 ER PT J AU Tiwari, S Wong, JM Heller, J Blann, P Abernethy, DR Soldatov, NM AF Tiwari, S Wong, JM Heller, J Blann, P Abernethy, DR Soldatov, NM TI Regulation of human Ca(V)1.2 calcium channel gene in atherosclerosis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, NIH, Baltimore, MD 21224 USA. Johns Hopkins Bayview Med Ctr, Div Vasc Surg, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A980 EP A980 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701054 ER PT J AU Tkaczyk, C Iwaki, S Metcalf'e, DD Horejsi, V Gilfillan, AM AF Tkaczyk, C Iwaki, S Metcalf'e, DD Horejsi, V Gilfillan, AM TI NTAL is at the crossroad between Kit and Fc epsilon RI-mediated signaling pathways in human mast cells (HuMCs) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. NIAID, Inst Mol Genet, NIH, Bethesda, MD 20892 USA. RI Horejsi, Vaclav/G-3113-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A789 EP A789 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700139 ER PT J AU Torres-Vazquez, J Gitler, AD Fraser, SD Berk, JA Pham, VN Fishman, MC Childs, S Epstein, JA Weinstein, BM AF Torres-Vazquez, J Gitler, AD Fraser, SD Berk, JA Pham, VN Fishman, MC Childs, S Epstein, JA Weinstein, BM TI Patterning and guidance of the vertebrate trunk angiogenic vasculature SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NICHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. Univ Penn, Div Cardiovasc, Philadelphia, PA 19104 USA. Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB T2N 1N4, Canada. Univ Calgary, Smooth Muscle Res Grp, Calgary, AB T2N 1N4, Canada. Massachusetts Gen Hosp, Cardiovasc Res Ctr, Charlestown, MA USA. Univ Penn, Sch Med, Mol Cardiol Res Ctr, Philadelphia, PA 19104 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A777 EP A777 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700080 ER PT J AU Tuo, JS Smith, B Chew, E Csaky, K Gery, I Chan, CC AF Tuo, JS Smith, B Chew, E Csaky, K Gery, I Chan, CC TI The involvement of sequence variation and expression of CX3CR1 in the pathogenesis of age-related macular degeneration SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Lab, Bethesda, MD 20892 USA. NEI, Div Epidemiol & Clin Res, Bethesda, MD 20892 USA. NEI, Lab Gene Therapy, Bethesda, MD 20892 USA. NR 0 TC 4 Z9 5 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A934 EP A934 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700837 ER PT J AU Uthus, EO Moskovitz, J AF Uthus, EO Moskovitz, J TI The specific activity of methionine sulfoxide reductase in mice is significantly affected by dietary selenium but not zinc SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. NHLBI, Biochem Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A916 EP A916 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700753 ER PT J AU Valencia, X He, LS Illei, G Lipsky, PE AF Valencia, X He, LS Illei, G Lipsky, PE TI CD4+CD25+T regulatory cells in autoimmune diseases SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. NIAMS, Off Clin Director, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A832 EP A832 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700354 ER PT J AU van Laar, JM Melchers, M Snapper, CM Sen, G Grivel, JC Lipsky, PE Grammer, AC AF van Laar, JM Melchers, M Snapper, CM Sen, G Grivel, JC Lipsky, PE Grammer, AC TI C-polysaccharide conjugated pneumococcal surface antigen protein A induces differentiation of human tonsillar memory B cells to Ig-secreting plasma cells. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20892 USA. NIAMS, Autoimmun Branch, NIH, Bethesda, MD 20893 USA. USUHS, Bethesda, MD USA. UHSHS, Bethesda, MD USA. NICHD, Lab Cellular & Mol Biophys, NIH, Bethesda, MD USA. RI Melchers, Mark/C-1438-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A820 EP A820 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700295 ER PT J AU Wang, W Merchlinsky, M Inman, J Golding, B AF Wang, W Merchlinsky, M Inman, J Golding, B TI Identification of a novel immunodominant CTL epitope derived from human factor VIII in a murine model of hemophilia A SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 US FDA, Ctr Biol Evaluat & Res, OBRR, DH, Bethesda, MD 20892 USA. US FDA, Ctr Biol Evaluat & Res, OVRR, Bethesda, MD 20892 USA. NIAID, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A826 EP A826 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700324 ER PT J AU Wang, WD Li, CL Nejsum, LN Thomsen, K Jonassen, T Knepper, MA Frokiaer, J Nielsen, S AF Wang, WD Li, CL Nejsum, LN Thomsen, K Jonassen, T Knepper, MA Frokiaer, J Nielsen, S TI Regulation of renal water and sodium excretion and AQP2 and ENaC localization in response to atrial natriuretic peptide (ANP) infusion in conscious euvolemic rats SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Aarhus Univ, Walter & Salt Res Ctr, DK-8000 Aarhus, Denmark. Aarhus Univ Hosp, DK-8000 Aarhus, Denmark. Univ Copenhagen, DK-1168 Copenhagen, Denmark. NHLBI, Kidney & Electrolyte Metab Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1019 EP A1019 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701242 ER PT J AU Wang, ZY Yang, D Chen, Q Oppenheim, JJ AF Wang, ZY Yang, D Chen, Q Oppenheim, JJ TI Pokeweed mitogen (PWM) induces chemotaxis, activation, and maturation of human dendritic cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 SAIC Frederick, Basic Res Program, Frederick, MD 21702 USA. NCI, Mol Immunoregulat Lab, Frederick, MD 21701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1147 EP A1147 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701852 ER PT J AU Wei, MY Cassano, PA Kritchevsky, S Harris, TB Holvoet, P Crapo, RO Jensen, R Newman, AB Bauer, DC AF Wei, MY Cassano, PA Kritchevsky, S Harris, TB Holvoet, P Crapo, RO Jensen, R Newman, AB Bauer, DC TI Oxidized LDL, antioxidants and pulmonary disease SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, Bethesda, MD 20892 USA. Wake Forest Univ, Sticht Ctr Aging, Winston Salem, NC 27109 USA. Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. Katholieke Univ Leuven, Ctr Expt Surg & Anesthesiol, Louvain, Belgium. Univ Utah, Sch Med, Div Pulm, Salt Lake City, UT 84112 USA. Univ Pittsburgh, Div Geriatr Med, Pittsburgh, PA 15260 USA. Univ Calif San Francisco, Sch Med, San Francisco, CA 94143 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A908 EP A908 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700716 ER PT J AU Weng, NP Lee, WH Li, Y Huston, G Swain, SL AF Weng, NP Lee, WH Li, Y Huston, G Swain, SL TI The gene expression profile of T helper (Th) cells as they transition from naive to effector to memory states reveals limited differences among the resting Th populations SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, NIH, Baltimore, MD 21224 USA. Trudeau Res Inst, Saranac Lake, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A797 EP A797 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700183 ER PT J AU Winkler-Pickett, RT Wiltrout, RH Young, HA Bere, EW Murphy, WE Ortaldo, JR AF Winkler-Pickett, RT Wiltrout, RH Young, HA Bere, EW Murphy, WE Ortaldo, JR TI Dissociation of NKT stimulation, cytokine induction and NK activation in vivo by the use of distinct TcR-binding ceramides SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, LEI, Frederick, MD 21702 USA. Univ Nevada, Sch Med, Reno, NV 89557 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1151 EP A1152 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701876 ER PT J AU Yang, D Chen, Q Kurosaka, K Howard, OMZ Rosenberg, HF Rybak, SM Kingsmore, SF Oppenheim, JJ AF Yang, D Chen, Q Kurosaka, K Howard, OMZ Rosenberg, HF Rybak, SM Kingsmore, SF Oppenheim, JJ TI Eosinophil-derived neurotoxin (EDN) is a selective chemoattractant and activator of dendritic cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 SAIC Frederick, Basic Res Program, Frederick, MD 21702 USA. NCI, Mol Immunoregulat Lab, Frederick, MD 21701 USA. NIAID, Host Def Lab, Bethesda, MD 20892 USA. NCI, Dev Therapeut Program, Frederick, MD 21701 USA. Mol Staging Inc, Basic Res Program, New Haven, CT USA. RI Howard, O M Zack/B-6117-2012 OI Howard, O M Zack/0000-0002-0505-7052 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1144 EP A1144 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701840 ER PT J AU Yazawa, H Ortaldo, J Back, T Subleski, J Wiltrout, R Watanabe, M AF Yazawa, H Ortaldo, J Back, T Subleski, J Wiltrout, R Watanabe, M TI Interleukin-15 gene therapy exclusively increases natural killer cells and suppresses tumor metastasis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Expt Immunol Lab, CCR, Ft Detrick, MD 21702 USA. SAIC, Expt Immunol Lab, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1152 EP A1152 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701877 ER PT J AU Zhang, X Young, HA AF Zhang, X Young, HA TI PPAR-gamma represses interferon-gamma gene expression by sequestration of CBP/p300 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Expt Immunol Lab, Frederick, MD 21702 USA. RI Young, Howard/A-6350-2008; Zhang, Xia/B-8152-2008 OI Young, Howard/0000-0002-3118-5111; Zhang, Xia/0000-0002-9040-1486 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1138 EP A1138 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701810 ER PT J AU Zhang, Z Dmitrieva, NI Levine, RL Park, JH Burg, MB AF Zhang, Z Dmitrieva, NI Levine, RL Park, JH Burg, MB TI Association of osmotic and oxidative stress: high urea or NaCl carbonylates renal inner medullary cell proteins in culture and in vivo SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHLBI, LKEM, Bethesda, MD 20892 USA. NHLBI, Biochem Lab, Bethesda, MD 20892 USA. NIDCR, Oral & Pharyngeal Canc Branch, Bethesda, MD 20892 USA. RI Levine, Rodney/D-9885-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A1032 EP A1032 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470701306 ER PT J AU Zhi, W Li, Y Weng, NP AF Zhi, W Li, Y Weng, NP TI IL-15 activates telomerase and preserves telomere length in long term cultured memory CD8+T cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, NIH, Baltimore, MD 21224 USA. NIA, Immunol Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A799 EP A799 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700191 ER PT J AU Zhou, XM Ferraris, JD Cai, Q Burg, MB AF Zhou, XM Ferraris, JD Cai, Q Burg, MB TI Reactive oxygen species (ROS) may contribute to activation of TonEBP/OREBP by high NaCl SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA. NIH, Kidney & Electrolyte Metab Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A954 EP A954 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700927 ER PT J AU Zhou, ZH Notkins, AL AF Zhou, ZH Notkins, AL TI Polyreactive antigen-binding B (PAB(+)) cells are widely distributed and the PAB(+) population consists of both B-1(+) and B-1(-) phenotypes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Natl Inst Dent & Craniofacial Res, Oral Infect & Immun Branch, Expt Med Sect, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A827 EP A828 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700332 ER PT J AU Zhu, JF Guo, LY Paul, WE AF Zhu, JF Guo, LY Paul, WE TI A conditional knockout of Gfi-1 reveals its importance in Th2 cell expansion SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 24 PY 2004 VL 18 IS 5 SU S BP A798 EP A798 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZB UT WOS:000220470700188 ER PT J AU Cai, T Fukushige, T Notkins, AL Krause, M AF Cai, T Fukushige, T Notkins, AL Krause, M TI Insulinoma-associated protein IA-2, a vesicle transmembrane protein, genetically interacts with UNC-31/CAPS and affects neurosecretion in Caenorhabditis elegans SO JOURNAL OF NEUROSCIENCE LA English DT Article DE autoantigen; diabetes; insulin; synaptic transmission; dense core vesicle; protein tyrosine phosphatase; PTP; aldicarb; dauer; C. elegans ID DEPENDENT DIABETES-MELLITUS; AGE-1 PI3 KINASE; C-ELEGANS; TYROSINE-PHOSPHATASE; MOLECULAR-CLONING; SYNAPTIC-TRANSMISSION; NERVOUS-SYSTEM; GENE FAMILY; EXOCYTOSIS; SECRETION AB IA-2 (insulinoma-associated protein 2), a major autoantigen in type 1 diabetes, is a receptor-tyrosine phosphatase-like protein associated with the membrane of secretory granules of neural and endocrine-specific cells. Loss of IA-2 activity in the mouse results in reduced insulin release and additional phenotypes, consistent with a general effect on neurosecretion and hormone release. To gain further insight into the cellular mechanisms of IA-2 function, we have studied the Caenorhabditis elegans homolog, CeIA-2 encoded by the ida-1 gene. Using two independent putative null alleles of ida-1, we demonstrate that animals lacking CeIA-2 activity are viable and exhibit subtle defects. Genetic studies of mutants in ida-1 and several genes involved in neurosecretory vesicle cargo release and signaling highlight two roles for CeIA-2. First, CeIA-2 has a specific and novel genetic interaction with UNC-31/CAPS, a protein that has been shown in other systems to regulate dense-core vesicle cargo release. Second, loss of CeIA-2 activity enhances weak alleles in the insulin-like signaling pathway. These results suggest that CeIA-2 may be an important factor in dense-core vesicle cargo release with parallels to insulin signaling in mammals. C1 NIDDKD, Sect Dev Biol, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. Natl Inst Dent & Craniofacial Res, Expt Med Sect, Oral Infect & Immun Branch, NIH, Bethesda, MD 20892 USA. RP Krause, M (reprint author), NIDDKD, Sect Dev Biol, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. EM mwkrause@helix.nih.gov OI Krause, Michael/0000-0001-6127-3940 NR 56 TC 45 Z9 68 U1 0 U2 0 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD MAR 24 PY 2004 VL 24 IS 12 BP 3115 EP 3124 DI 10.1523/JNEUROSCI.0101-04.2004 PG 10 WC Neurosciences SC Neurosciences & Neurology GA 807KR UT WOS:000220500900028 PM 15044551 ER PT J AU Gruschus, JM Han, CJ Greener, T Ferretti, JA Greene, LE Eisenberg, E AF Gruschus, JM Han, CJ Greener, T Ferretti, JA Greene, LE Eisenberg, E TI Structure of the functional fragment of auxilin required for catalytic uncoating of clathrin-coated vesicles SO BIOCHEMISTRY LA English DT Article ID ESCHERICHIA-COLI DNAJ; J-DOMAIN; MOLECULAR CHAPERONE; SECONDARY STRUCTURE; ATPASE ACTIVITY; NMR STRUCTURE; IN-VIVO; NOESY-HMQC; PROTEIN; HSC70 AB The three-dimensional structure of the C-terminal 20 kDa portion of auxilin, which consists of the clathrin binding region and the C-terminal J-domain, has been determined by NMR. Auxilin is an Hsp40 family protein that catalytically supports the uncoating of clathrin-coated vesicles through recruitment of Hsc70 in an ATP hydrolysis-driven process. This 20 kDa auxilin construct contains the minimal sequential region required to uncoat clathrin-coated vesicles catalytically. The tertiary structure consists of six helices, where the first three are unique to auxilin and believed to be important in the catalytic uncoating of clathrin. The last three helices correspond to the canonical J-domain of Hsp40 proteins. The first helix, helix 1, which contains a conserved FEDLL motif believed to be necessary for clathrin binding, is transient and not packed against the rest of the structure. Helix 1 is joined to helix 2 by a flexible linker. Helix 2 packs loosely against the J-domain surface, whereas helix 3 packs tightly and makes critical contributions to the J-domain core. A long insert loop, also unique to the auxilin J-domain, is seen between helix 4 and helix 5. Comparison with a previously reported structure of auxilin containing only helices 3-6 shows a significant difference in the invariant HPD segment of the J-domain. The region where helix 1 is located corresponds to the expected region of the unstructured G/F-rich domain seen in DnaJ, i.e., the canonical N-terminal J-domain protein. In contrast, the location of helix 1 differs from the substrate binding regions of two other Hsp40 proteins, Escherichia coli Hsc20 and viral large T antigen. The variety of biological functions performed by Hsp40 proteins such as auxilin, as well as the observed differences in the structure and function of their substrate binding regions, supports the notion that Hsp40 proteins act as target-specific adaptors that recruit their more general Hsp70 partners to specific biological roles. C1 NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. NHLBI, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. RP Eisenberg, E (reprint author), NHLBI, Biophys Chem Lab, NIH, Bldg 50,Rm 2525, Bethesda, MD 20892 USA. EM eisenbee@nhlbi.nih.gov NR 50 TC 23 Z9 24 U1 0 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 23 PY 2004 VL 43 IS 11 BP 3111 EP 3119 DI 10.1021/bi0354740 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 804CL UT WOS:000220276700013 PM 15023062 ER PT J AU Wilkerson, AJP Teeling, EC Troyer, JL Bar-Gal, GK Roelke, M Marker, L Pecon-Slattery, J O'Brien, SJ AF Wilkerson, AJP Teeling, EC Troyer, JL Bar-Gal, GK Roelke, M Marker, L Pecon-Slattery, J O'Brien, SJ TI Coronavirus outbreak in cheetahs: Lessons for SARS SO CURRENT BIOLOGY LA English DT Letter C1 NCI, Lab Genomic Divers, SAIC, Frederick, MD 21701 USA. RP O'Brien, SJ (reprint author), NCI, Lab Genomic Divers, SAIC, Frederick, MD 21701 USA. EM obrien@ncifcrf.gov RI Troyer, Jennifer/B-8415-2012 NR 9 TC 11 Z9 12 U1 0 U2 1 PU CELL PRESS PI CAMBRIDGE PA 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD MAR 23 PY 2004 VL 14 IS 6 BP R227 EP R228 PG 2 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 808RZ UT WOS:000220587500007 ER PT J AU Zimmerberg, J McLaughlin, S AF Zimmerberg, J McLaughlin, S TI Membrane curvature: How BAR domains bend bilayers SO CURRENT BIOLOGY LA English DT Editorial Material ID BIOLOGICAL-MEMBRANES; ORGANIZATION; AMPHIPHYSIN; ENDOCYTOSIS; PROTEINS; FISSION; COUPLES AB An important new structure suggests the BAR domain is a membrane-binding module that can both produce and sense membrane curvature. BAR resembles a banana that binds membranes electrostatically through its positively charged, concave surface. C1 NICHHD, Lab Cellular & Mol Biophys, NIH, Bethesda, MD 20892 USA. SUNY Stony Brook, Hlth Sci Ctr, Dept Physiol & Biophys, Stony Brook, NY 11794 USA. RP Zimmerberg, J (reprint author), NICHHD, Lab Cellular & Mol Biophys, NIH, Bethesda, MD 20892 USA. NR 18 TC 102 Z9 106 U1 1 U2 11 PU CELL PRESS PI CAMBRIDGE PA 1100 MASSACHUSETTS AVE, CAMBRIDGE, MA 02138 USA SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD MAR 23 PY 2004 VL 14 IS 6 BP R250 EP R252 DI 10.1016/j.cub.2004.02.060 PG 3 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 808RZ UT WOS:000220587500016 PM 15043839 ER PT J AU Andrews, KW Holden, JM Dwyer, J Picciano, MF Zhao, CWL AF Andrews, KW Holden, JM Dwyer, J Picciano, MF Zhao, CWL TI Priority products for the dietary supplement ingredient database SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. ARS, Nutrient Data Lab, USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A127 EP A128 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600619 ER PT J AU Aoki, J Konno, A Candotti, F Schwartzberg, PL AF Aoki, J Konno, A Candotti, F Schwartzberg, PL TI Comparing the role of WASP and Itk in T helper cell differentiation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHGRI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A54 EP A54 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600265 ER PT J AU Babu, S Kumaraswami, V Nutman, TB AF Babu, S Kumaraswami, V Nutman, TB TI Decreased T- and B-cell expression of Toll-like receptors in patent lymphatic filarial infection SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 LPD, NIH, Bethesda, MD 20892 USA. TB Res Ctr, Madras, Tamil Nadu, India. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A417 EP A417 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602010 ER PT J AU Baird, AE AF Baird, AE TI Neuroimaging of stroke SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NINDS, Stroke Neurosci Unit, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A404 EP A404 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470601945 ER PT J AU Bansal, G Rao, S Nocka, K Druey, K AF Bansal, G Rao, S Nocka, K Druey, K TI Mast cells express multiple regulator of G protein signaling (RGS) proteins SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Lab Allerg Dis, NIH, Rockville, MD 20852 USA. UCB Res Inc, UCB Pharma, Cambridge, MA USA. RI Bansal, Geetanjali/C-3854-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A219 EP A219 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470601052 ER PT J AU Bauer, B Hartz, AMS Fricker, G Miller, DS AF Bauer, B Hartz, AMS Fricker, G Miller, DS TI Ligands of the nuclear xenobiotic receptor, PXR, upregulate p-glycoprotein expression at the blood-brain barrier SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIEHS, LPC, Res Triangle Pk, NC USA. Univ Heidelberg, Inst Pharm & Mol Biotechnol, Heidelberg, Germany. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A676 EP A676 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470603245 ER PT J AU Bazinet, RP Douglas, H McMillan, EG Wilkie, BN Cunnane, SC AF Bazinet, RP Douglas, H McMillan, EG Wilkie, BN Cunnane, SC TI Dietary 18 : 3 omega 3 influences immune function and the tissue fatty acid response to antigens and adjuvant in the pig SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, Brain Physiol & Metab Sect, NIH, Bethesda, MD 20892 USA. Univ Toronto, Toronto, ON, Canada. Maple Leaf Foods Inc, Shur Gain Agres, Guelph, ON, Canada. Univ Guelph, Guelph, ON N1G 2W1, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A160 EP A160 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600773 ER PT J AU Beigi, F Chakir, K Xiao, RP Wainer, IW AF Beigi, F Chakir, K Xiao, RP Wainer, IW TI Characterization of beta2-adrenergic receptor immobilized affinity chromatography, stationary phase SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, GRC, LCI, NIH, Baltimore, MD 21224 USA. NIA, GRC, LCS, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A218 EP A218 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470601047 ER PT J AU Bentil, SA Mager, D Soldatov, NM Kobrinsky, E AF Bentil, SA Mager, D Soldatov, NM Kobrinsky, E TI Defining regions of interest in FRET microscopy via statistical analysis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIA, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A225 EP A226 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470601082 ER PT J AU Bindewald, BK Radimer, K Picciano, MF Swanson, C AF Bindewald, BK Radimer, K Picciano, MF Swanson, C TI Non-supplements reported as dietary supplements in the National Health and Nutrition Examination Survey 1999-2000 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 DHANES, Natl Ctr Hlth Stat, Hyattsville, MD 20872 USA. ODS, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A112 EP A112 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600544 ER PT J AU Bindewald, BK Radimer, K Picciano, MF Swanson, C AF Bindewald, BK Radimer, K Picciano, MF Swanson, C TI The dietary supplement database of the National Health and Nutrition Examination Survey SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 DHANES, Natl Ctr Hlth Stat, Hyattsville, MD 20872 USA. ODS, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A112 EP A112 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600545 ER PT J AU Blumenkron, F Lipsky, PE Grammer, AC AF Blumenkron, F Lipsky, PE Grammer, AC TI Bidirectional modulatory influences of CD154/CD40-Ligand expressed on the surface of activated lymphocytes during T Cell-B cell collaboration SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAMS, Autoimmun Branch, B Cell Grp, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A434 EP A434 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602095 ER PT J AU Borthakur, G Sprandel, K Bowen, PE Sapuntzakis, MS Gustin, D Crowell, J Rodvold, K AF Borthakur, G Sprandel, K Bowen, PE Sapuntzakis, MS Gustin, D Crowell, J Rodvold, K TI Multiple dose phamacokinetic (PK) study of lycopene supplementation from a tomato paste-oil mixture SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 Univ Illinois, Chicago, IL 60612 USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A156 EP A156 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600757 ER PT J AU Bosticardo, M Novelli, F Casanova, JL Candotti, F AF Bosticardo, M Novelli, F Casanova, JL Candotti, F TI Restoration of IL-12 signaling pathway in IL-12 receptor beta-1 (IL-12R beta 1) KO mice after retroviral-mediated gene transfer into hematopoietic stem cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHGRI, GMBB, NIH, Bethesda, MD 20892 USA. San Giovanni Battista Hosp, CeRMS, Turin, Italy. Univ Paris 05, INSERM U550, Fac Med Necker, Lab Genet Humaine Maladies Infect, F-75270 Paris 06, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A47 EP A47 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600229 ER PT J AU Braun, DC Cao, Y Wang, SM Garfield, S Hur, GM Blumberg, PM AF Braun, DC Cao, Y Wang, SM Garfield, S Hur, GM Blumberg, PM TI Kinetics of uptake and localization of fluorescent phorbol ester and protein kinase C or RasGRP3 as a function of time after phorbol ester addition SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Lab Cellular Carcinogenesis & Tumor, NIH, Bethesda, MD 20892 USA. Univ Michigan, Ctr Canc, Ann Arbor, MI USA. NCI, Lab Expt Carcinogenesis, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A573 EP A573 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602748 ER PT J AU Braun, DC Cao, Y Wang, SM Garfield, S Hur, GM Blumberg, PM AF Braun, DC Cao, Y Wang, SM Garfield, S Hur, GM Blumberg, PM TI Kinetics of uptake and localization of fluorescent phorbol ester and protein kinase C or RasGRP3 as a function of time after phorbol ester addition SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NCI, Cellular Carcinogenesis & Tumor Promot Lab, NIH, Bethesda, MD 20892 USA. Univ Michigan, Ctr Canc, Ann Arbor, MI 48109 USA. NCI, Expt Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A222 EP A222 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470601066 ER PT J AU Breslow, RA Smothers, BA AF Breslow, RA Smothers, BA TI Alcohol and body mass index (BMI) in never-smokers SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAAA, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A115 EP A115 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600559 ER PT J AU Cannons, JL Hill, B Jankovic, D Sher, A Schwartzberg, PL AF Cannons, JL Hill, B Jankovic, D Sher, A Schwartzberg, PL TI The role of SAP in T helper cell differentiation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NHGRI, NIH, Bethesda, MD 20815 USA. George Washington Univ, Inst Med Sci, Washington, DC 20052 USA. NAIAD, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A57 EP A57 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470600283 ER PT J AU Chan, CC Smith, JA Shen, DF LeHoang, P Font, R Grossniklaus, HE AF Chan, CC Smith, JA Shen, DF LeHoang, P Font, R Grossniklaus, HE TI Helicobacter pylori (H-pylori) genes in conjunctival mucosaassociated lymphoid tissue (MALT) lymphoma SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Sect, Immunol Lab, Natl Inst Hlth, Bethesda, MD 20892 USA. Hop La Pitie Salpetriere, Paris, France. Baylor Coll Med, Houston, TX 77030 USA. Emory Univ, Ctr Eye, Atlanta, GA 30322 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A563 EP A563 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602700 ER PT J AU Chapman, S Ramchandani, M Sereti, I AF Chapman, S Ramchandani, M Sereti, I TI Naive CD4+/CD25+ T cells in peripheral blood represent the product of cytokine-driven homeostatic proliferation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIAID, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A467 EP A467 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602250 ER PT J AU Chen, J Vistica, BP Ham, DI Fariss, RN Wawrousek, EF Chan, CC Gery, I AF Chen, J Vistica, BP Ham, DI Fariss, RN Wawrousek, EF Chan, CC Gery, I TI Down-regulation of chemokine receptor CXCR3 by inflammation-inducing Th1 cells upon reactivation by the Ag in vitro and in vivo SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NEI, Immunol Lab, NIH, Bethesda, MD USA. NEI, Lab Mechanisms Ocular Dis, NIH, Bethesda, MD USA. NEI, Mol & Dev Biol Lab, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A448 EP A448 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602161 ER PT J AU Chen, X Krakauer, T Oppenheim, JJ Howard, OMZ AF Chen, X Krakauer, T Oppenheim, JJ Howard, OMZ TI Yin Zi Huang, an injectable multi-component Chinese herbal medicine, is a potent inhibitor of T cell activation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 SAIC Frederick Inc, Mol Immunoregulat Lab, NCI, Ft Detrick, MD 21702 USA. USAMRIID, Dept Immunol & Mol Biol, Ft Detrick, MD 21702 USA. NCI, Mol Immunoregulat Lab, Ft Detrick, MD 21702 USA. RI Howard, O M Zack/B-6117-2012; Chen, Xin/I-6601-2015 OI Howard, O M Zack/0000-0002-0505-7052; Chen, Xin/0000-0002-2628-4027 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A439 EP A440 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470602121 ER PT J AU Chiueh, CC Andoh, T Chock, B AF Chiueh, CC Andoh, T Chock, B TI Role of cyclic G-MIP-dependent thioredoxin expression in preconditioning-induced hormesis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology 2004 Meeting CY APR 17-21, 2004 CL Washington, DC C1 NIMH, LCS, NIH, Bethesda, MD 20892 USA. Toyama Med & Pharma Sci Univ, Toyama, Japan. NHLBI, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 23 PY 2004 VL 18 IS 4 SU S BP A211 EP A211 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 806ZA UT WOS:000220470601013 ER EF